LOCUS       DAZFWL020000001       112733 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFWL020000001 DAZFWL020000000
VERSION     DAZFWL020000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 112733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 112733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 112733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..112733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.001"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..251)
                     /locus_tag="ACHKNP_RS00005"
                     /old_locus_tag="pgaptmp_000001"
     CDS             complement(<1..251)
                     /locus_tag="ACHKNP_RS00005"
                     /old_locus_tag="pgaptmp_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU4702252.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNS"
     gene            complement(294..1091)
                     /gene="rfaP"
                     /locus_tag="ACHKNP_RS00010"
                     /old_locus_tag="pgaptmp_000002"
     CDS             complement(294..1091)
                     /gene="rfaP"
                     /locus_tag="ACHKNP_RS00010"
                     /old_locus_tag="pgaptmp_000002"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HGU4702253.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMHGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(1084..2208)
                     /gene="waaG"
                     /locus_tag="ACHKNP_RS00015"
                     /old_locus_tag="pgaptmp_000003"
     CDS             complement(1084..2208)
                     /gene="waaG"
                     /locus_tag="ACHKNP_RS00015"
                     /old_locus_tag="pgaptmp_000003"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU4702254.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVCSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAPYIVEANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARYYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(2205..3263)
                     /gene="rfaQ"
                     /locus_tag="ACHKNP_RS00020"
                     /old_locus_tag="pgaptmp_000004"
     CDS             complement(2205..3263)
                     /gene="rfaQ"
                     /locus_tag="ACHKNP_RS00020"
                     /old_locus_tag="pgaptmp_000004"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HGU4702255.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            3676..4953
                     /gene="waaA"
                     /locus_tag="ACHKNP_RS00025"
                     /old_locus_tag="pgaptmp_000005"
     CDS             3676..4953
                     /gene="waaA"
                     /locus_tag="ACHKNP_RS00025"
                     /old_locus_tag="pgaptmp_000005"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HGU4702256.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVSALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4961..5440
                     /gene="coaD"
                     /locus_tag="ACHKNP_RS00030"
                     /old_locus_tag="pgaptmp_000006"
     CDS             4961..5440
                     /gene="coaD"
                     /locus_tag="ACHKNP_RS00030"
                     /old_locus_tag="pgaptmp_000006"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGU4702257.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(5479..6288)
                     /gene="mutM"
                     /locus_tag="ACHKNP_RS00035"
                     /old_locus_tag="pgaptmp_000007"
     CDS             complement(5479..6288)
                     /gene="mutM"
                     /locus_tag="ACHKNP_RS00035"
                     /old_locus_tag="pgaptmp_000007"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HGU4702258.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(6386..6553)
                     /gene="rpmG"
                     /locus_tag="ACHKNP_RS00040"
                     /old_locus_tag="pgaptmp_000008"
     CDS             complement(6386..6553)
                     /gene="rpmG"
                     /locus_tag="ACHKNP_RS00040"
                     /old_locus_tag="pgaptmp_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGU4702259.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(6574..6810)
                     /gene="rpmB"
                     /locus_tag="ACHKNP_RS00045"
                     /old_locus_tag="pgaptmp_000009"
     CDS             complement(6574..6810)
                     /gene="rpmB"
                     /locus_tag="ACHKNP_RS00045"
                     /old_locus_tag="pgaptmp_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGU4702260.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(7027..7695)
                     /gene="radC"
                     /locus_tag="ACHKNP_RS00050"
                     /old_locus_tag="pgaptmp_000010"
     CDS             complement(7027..7695)
                     /gene="radC"
                     /locus_tag="ACHKNP_RS00050"
                     /old_locus_tag="pgaptmp_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGU4702261.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            7867..9087
                     /gene="coaBC"
                     /locus_tag="ACHKNP_RS00055"
                     /old_locus_tag="pgaptmp_000011"
     CDS             7867..9087
                     /gene="coaBC"
                     /locus_tag="ACHKNP_RS00055"
                     /old_locus_tag="pgaptmp_000011"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HGU4702262.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            9065..9523
                     /gene="dut"
                     /locus_tag="ACHKNP_RS00060"
                     /old_locus_tag="pgaptmp_000012"
     CDS             9065..9523
                     /gene="dut"
                     /locus_tag="ACHKNP_RS00060"
                     /old_locus_tag="pgaptmp_000012"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGU4702263.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            9630..10226
                     /gene="slmA"
                     /locus_tag="ACHKNP_RS00065"
                     /old_locus_tag="pgaptmp_000013"
     CDS             9630..10226
                     /gene="slmA"
                     /locus_tag="ACHKNP_RS00065"
                     /old_locus_tag="pgaptmp_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HGU4702264.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(10263..10904)
                     /gene="pyrE"
                     /locus_tag="ACHKNP_RS00070"
                     /old_locus_tag="pgaptmp_000014"
     CDS             complement(10263..10904)
                     /gene="pyrE"
                     /locus_tag="ACHKNP_RS00070"
                     /old_locus_tag="pgaptmp_000014"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGU4702265.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(10970..11686)
                     /gene="rph"
                     /locus_tag="ACHKNP_RS00075"
                     /old_locus_tag="pgaptmp_000015"
     CDS             complement(10970..11686)
                     /gene="rph"
                     /locus_tag="ACHKNP_RS00075"
                     /old_locus_tag="pgaptmp_000015"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HGU4702266.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            11813..12676
                     /gene="yicC"
                     /locus_tag="ACHKNP_RS00080"
                     /old_locus_tag="pgaptmp_000016"
     CDS             11813..12676
                     /gene="yicC"
                     /locus_tag="ACHKNP_RS00080"
                     /old_locus_tag="pgaptmp_000016"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HGU4702267.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            12897..13721
                     /gene="dinD"
                     /locus_tag="ACHKNP_RS00085"
                     /old_locus_tag="pgaptmp_000017"
     CDS             12897..13721
                     /gene="dinD"
                     /locus_tag="ACHKNP_RS00085"
                     /old_locus_tag="pgaptmp_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HGU4702268.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            14013..14630
                     /gene="yicG"
                     /locus_tag="ACHKNP_RS00090"
                     /old_locus_tag="pgaptmp_000018"
     CDS             14013..14630
                     /gene="yicG"
                     /locus_tag="ACHKNP_RS00090"
                     /old_locus_tag="pgaptmp_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HGU4702269.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(14627..16309)
                     /gene="ligB"
                     /locus_tag="ACHKNP_RS00095"
                     /old_locus_tag="pgaptmp_000019"
     CDS             complement(14627..16309)
                     /gene="ligB"
                     /locus_tag="ACHKNP_RS00095"
                     /old_locus_tag="pgaptmp_000019"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HGU4702270.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            16567..17190
                     /gene="gmk"
                     /locus_tag="ACHKNP_RS00100"
                     /old_locus_tag="pgaptmp_000020"
     CDS             16567..17190
                     /gene="gmk"
                     /locus_tag="ACHKNP_RS00100"
                     /old_locus_tag="pgaptmp_000020"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGU4702271.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17245..17520
                     /gene="rpoZ"
                     /locus_tag="ACHKNP_RS00105"
                     /old_locus_tag="pgaptmp_000021"
     CDS             17245..17520
                     /gene="rpoZ"
                     /locus_tag="ACHKNP_RS00105"
                     /old_locus_tag="pgaptmp_000021"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGU4702272.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            17539..19647
                     /gene="spoT"
                     /locus_tag="ACHKNP_RS00110"
                     /old_locus_tag="pgaptmp_000022"
     CDS             17539..19647
                     /gene="spoT"
                     /locus_tag="ACHKNP_RS00110"
                     /old_locus_tag="pgaptmp_000022"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HGU4702273.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            19654..20343
                     /gene="trmH"
                     /locus_tag="ACHKNP_RS00115"
                     /old_locus_tag="pgaptmp_000023"
     CDS             19654..20343
                     /gene="trmH"
                     /locus_tag="ACHKNP_RS00115"
                     /old_locus_tag="pgaptmp_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HGU4702274.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            20349..22430
                     /gene="recG"
                     /locus_tag="ACHKNP_RS00120"
                     /old_locus_tag="pgaptmp_000024"
     CDS             20349..22430
                     /gene="recG"
                     /locus_tag="ACHKNP_RS00120"
                     /old_locus_tag="pgaptmp_000024"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGU4702275.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVHHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(22415..23281)
                     /locus_tag="ACHKNP_RS00125"
                     /old_locus_tag="pgaptmp_000025"
     CDS             complement(22415..23281)
                     /locus_tag="ACHKNP_RS00125"
                     /old_locus_tag="pgaptmp_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702276.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(23284..24489)
                     /gene="gltS"
                     /locus_tag="ACHKNP_RS00130"
                     /old_locus_tag="pgaptmp_000026"
     CDS             complement(23284..24489)
                     /gene="gltS"
                     /locus_tag="ACHKNP_RS00130"
                     /old_locus_tag="pgaptmp_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGU4702277.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            24769..26160
                     /gene="xanP"
                     /locus_tag="ACHKNP_RS00135"
                     /old_locus_tag="pgaptmp_000027"
     CDS             24769..26160
                     /gene="xanP"
                     /locus_tag="ACHKNP_RS00135"
                     /old_locus_tag="pgaptmp_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HGU4702278.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            26281..27990
                     /gene="yicH"
                     /locus_tag="ACHKNP_RS00140"
                     /old_locus_tag="pgaptmp_000028"
     CDS             26281..27990
                     /gene="yicH"
                     /locus_tag="ACHKNP_RS00140"
                     /old_locus_tag="pgaptmp_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU4702279.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRINGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(28043..30361)
                     /gene="yicI"
                     /locus_tag="ACHKNP_RS00145"
                     /old_locus_tag="pgaptmp_000029"
     CDS             complement(28043..30361)
                     /gene="yicI"
                     /locus_tag="ACHKNP_RS00145"
                     /old_locus_tag="pgaptmp_000029"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HGU4702280.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGNNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(30371..31753)
                     /gene="yicJ"
                     /locus_tag="ACHKNP_RS00150"
                     /old_locus_tag="pgaptmp_000030"
     CDS             complement(30371..31753)
                     /gene="yicJ"
                     /locus_tag="ACHKNP_RS00150"
                     /old_locus_tag="pgaptmp_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HGU4702281.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            32046..32140
                     /locus_tag="ACHKNP_RS00155"
                     /old_locus_tag="pgaptmp_000031"
     tRNA            32046..32140
                     /locus_tag="ACHKNP_RS00155"
                     /old_locus_tag="pgaptmp_000031"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32080..32082,aa:Sec,seq:tca)
     gene            32219..32347
                     /gene="rhuM"
                     /locus_tag="ACHKNP_RS00160"
                     /old_locus_tag="pgaptmp_000032"
                     /pseudo
     CDS             32219..32347
                     /gene="rhuM"
                     /locus_tag="ACHKNP_RS00160"
                     /old_locus_tag="pgaptmp_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            32776..33960
                     /gene="setC"
                     /locus_tag="ACHKNP_RS00165"
                     /old_locus_tag="pgaptmp_000033"
     CDS             32776..33960
                     /gene="setC"
                     /locus_tag="ACHKNP_RS00165"
                     /old_locus_tag="pgaptmp_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HGU4702282.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLGDE
                     LKARPIMVDFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMICIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            34071..34994
                     /gene="yicL"
                     /locus_tag="ACHKNP_RS00170"
                     /old_locus_tag="pgaptmp_000034"
     CDS             34071..34994
                     /gene="yicL"
                     /locus_tag="ACHKNP_RS00170"
                     /old_locus_tag="pgaptmp_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HGU4702283.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(34998..35816)
                     /gene="nlpA"
                     /locus_tag="ACHKNP_RS00175"
                     /old_locus_tag="pgaptmp_000035"
     CDS             complement(34998..35816)
                     /gene="nlpA"
                     /locus_tag="ACHKNP_RS00175"
                     /old_locus_tag="pgaptmp_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HGU4702284.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            36038..36331
                     /gene="yicS"
                     /locus_tag="ACHKNP_RS00180"
                     /old_locus_tag="pgaptmp_000036"
     CDS             36038..36331
                     /gene="yicS"
                     /locus_tag="ACHKNP_RS00180"
                     /old_locus_tag="pgaptmp_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HGU4702285.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIHEAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(36372..37562)
                     /gene="nepI"
                     /locus_tag="ACHKNP_RS00185"
                     /old_locus_tag="pgaptmp_000037"
     CDS             complement(36372..37562)
                     /gene="nepI"
                     /locus_tag="ACHKNP_RS00185"
                     /old_locus_tag="pgaptmp_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HGU4702286.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(37773..38225)
                     /gene="yicN"
                     /locus_tag="ACHKNP_RS00190"
                     /old_locus_tag="pgaptmp_000038"
     CDS             complement(37773..38225)
                     /gene="yicN"
                     /locus_tag="ACHKNP_RS00190"
                     /old_locus_tag="pgaptmp_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HGU4702287.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(38278..39612)
                     /gene="adeQ"
                     /locus_tag="ACHKNP_RS00195"
                     /old_locus_tag="pgaptmp_000039"
                     /gene_synonym="yicO"
     CDS             complement(38278..39612)
                     /gene="adeQ"
                     /locus_tag="ACHKNP_RS00195"
                     /old_locus_tag="pgaptmp_000039"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HGU4702288.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            39787..41553
                     /gene="adeD"
                     /locus_tag="ACHKNP_RS00200"
                     /old_locus_tag="pgaptmp_000040"
     CDS             39787..41553
                     /gene="adeD"
                     /locus_tag="ACHKNP_RS00200"
                     /old_locus_tag="pgaptmp_000040"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HGU4702289.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(41598..42989)
                     /gene="uhpT"
                     /locus_tag="ACHKNP_RS00205"
                     /old_locus_tag="pgaptmp_000041"
     CDS             complement(41598..42989)
                     /gene="uhpT"
                     /locus_tag="ACHKNP_RS00205"
                     /old_locus_tag="pgaptmp_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HGU4702290.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(43245..44564)
                     /gene="uhpC"
                     /locus_tag="ACHKNP_RS00210"
                     /old_locus_tag="pgaptmp_000042"
     CDS             complement(43245..44564)
                     /gene="uhpC"
                     /locus_tag="ACHKNP_RS00210"
                     /old_locus_tag="pgaptmp_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGU4702291.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGVSALLLLPFLNAQAPREA"
     gene            complement(44574..46076)
                     /gene="uhpB"
                     /locus_tag="ACHKNP_RS00215"
                     /old_locus_tag="pgaptmp_000043"
     CDS             complement(44574..46076)
                     /gene="uhpB"
                     /locus_tag="ACHKNP_RS00215"
                     /old_locus_tag="pgaptmp_000043"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HGU4702292.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(46076..46666)
                     /gene="uhpA"
                     /locus_tag="ACHKNP_RS00220"
                     /old_locus_tag="pgaptmp_000044"
     CDS             complement(46076..46666)
                     /gene="uhpA"
                     /locus_tag="ACHKNP_RS00220"
                     /old_locus_tag="pgaptmp_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HGU4702293.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(46741..47031)
                     /gene="ilvN"
                     /locus_tag="ACHKNP_RS00225"
                     /old_locus_tag="pgaptmp_000045"
     CDS             complement(46741..47031)
                     /gene="ilvN"
                     /locus_tag="ACHKNP_RS00225"
                     /old_locus_tag="pgaptmp_000045"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU4702294.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(47035..48723)
                     /gene="ilvB"
                     /locus_tag="ACHKNP_RS00230"
                     /old_locus_tag="pgaptmp_000046"
     CDS             complement(47035..48723)
                     /gene="ilvB"
                     /locus_tag="ACHKNP_RS00230"
                     /old_locus_tag="pgaptmp_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGU4702295.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDVFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(48829..48927)
                     /gene="ivbL"
                     /locus_tag="ACHKNP_RS00235"
                     /old_locus_tag="pgaptmp_000047"
     CDS             complement(48829..48927)
                     /gene="ivbL"
                     /locus_tag="ACHKNP_RS00235"
                     /old_locus_tag="pgaptmp_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HGU4702296.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            49492..49581
                     /gene="tisB"
                     /locus_tag="ACHKNP_RS00240"
                     /old_locus_tag="pgaptmp_000048"
     CDS             49492..49581
                     /gene="tisB"
                     /locus_tag="ACHKNP_RS00240"
                     /old_locus_tag="pgaptmp_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HGU4702297.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(49844..49918)
                     /gene="ysdE"
                     /locus_tag="ACHKNP_RS00245"
                     /old_locus_tag="pgaptmp_000049"
     CDS             complement(49844..49918)
                     /gene="ysdE"
                     /locus_tag="ACHKNP_RS00245"
                     /old_locus_tag="pgaptmp_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HGU4702298.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            50000..51184
                     /gene="emrD"
                     /locus_tag="ACHKNP_RS00250"
                     /old_locus_tag="pgaptmp_000050"
     CDS             50000..51184
                     /gene="emrD"
                     /locus_tag="ACHKNP_RS00250"
                     /old_locus_tag="pgaptmp_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HGU4702299.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(51192..51689)
                     /gene="yidF"
                     /locus_tag="ACHKNP_RS00255"
                     /old_locus_tag="pgaptmp_000051"
     CDS             complement(51192..51689)
                     /gene="yidF"
                     /locus_tag="ACHKNP_RS00255"
                     /old_locus_tag="pgaptmp_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGU4702300.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(51686..52048)
                     /gene="yidG"
                     /locus_tag="ACHKNP_RS00260"
                     /old_locus_tag="pgaptmp_000052"
     CDS             complement(51686..52048)
                     /gene="yidG"
                     /locus_tag="ACHKNP_RS00260"
                     /old_locus_tag="pgaptmp_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HGU4702301.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(52038..52385)
                     /gene="yidH"
                     /locus_tag="ACHKNP_RS00265"
                     /old_locus_tag="pgaptmp_000053"
     CDS             complement(52038..52385)
                     /gene="yidH"
                     /locus_tag="ACHKNP_RS00265"
                     /old_locus_tag="pgaptmp_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HGU4702302.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            52495..52944
                     /gene="yidI"
                     /locus_tag="ACHKNP_RS00270"
                     /old_locus_tag="pgaptmp_000054"
     CDS             52495..52944
                     /gene="yidI"
                     /locus_tag="ACHKNP_RS00270"
                     /old_locus_tag="pgaptmp_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HGU4702303.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(52991..54484)
                     /gene="yidJ"
                     /locus_tag="ACHKNP_RS00275"
                     /old_locus_tag="pgaptmp_000055"
     CDS             complement(52991..54484)
                     /gene="yidJ"
                     /locus_tag="ACHKNP_RS00275"
                     /old_locus_tag="pgaptmp_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4702304.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDGLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(54481..56196)
                     /gene="yidK"
                     /locus_tag="ACHKNP_RS00280"
                     /old_locus_tag="pgaptmp_000056"
     CDS             complement(54481..56196)
                     /gene="yidK"
                     /locus_tag="ACHKNP_RS00280"
                     /old_locus_tag="pgaptmp_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HGU4702305.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            56363..57256
                     /gene="yidL"
                     /locus_tag="ACHKNP_RS00285"
                     /old_locus_tag="pgaptmp_000057"
     CDS             56363..57256
                     /gene="yidL"
                     /locus_tag="ACHKNP_RS00285"
                     /old_locus_tag="pgaptmp_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4702306.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(57253..58067)
                     /locus_tag="ACHKNP_RS00290"
                     /old_locus_tag="pgaptmp_000058"
                     /pseudo
     CDS             complement(57253..58067)
                     /locus_tag="ACHKNP_RS00290"
                     /old_locus_tag="pgaptmp_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(58067..59683)
                     /locus_tag="ACHKNP_RS00295"
                     /old_locus_tag="pgaptmp_000059"
     CDS             complement(58067..59683)
                     /locus_tag="ACHKNP_RS00295"
                     /old_locus_tag="pgaptmp_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4702307.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            59979..60695
                     /gene="yidP"
                     /locus_tag="ACHKNP_RS00300"
                     /old_locus_tag="pgaptmp_000060"
     CDS             59979..60695
                     /gene="yidP"
                     /locus_tag="ACHKNP_RS00300"
                     /old_locus_tag="pgaptmp_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4702308.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(60692..62353)
                     /gene="yidE"
                     /locus_tag="ACHKNP_RS00305"
                     /old_locus_tag="pgaptmp_000061"
     CDS             complement(60692..62353)
                     /gene="yidE"
                     /locus_tag="ACHKNP_RS00305"
                     /old_locus_tag="pgaptmp_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HGU4702309.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDMPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(62550..62978)
                     /gene="ibpB"
                     /locus_tag="ACHKNP_RS00310"
                     /old_locus_tag="pgaptmp_000062"
     CDS             complement(62550..62978)
                     /gene="ibpB"
                     /locus_tag="ACHKNP_RS00310"
                     /old_locus_tag="pgaptmp_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HGU4702310.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(63090..63503)
                     /gene="ibpA"
                     /locus_tag="ACHKNP_RS00315"
                     /old_locus_tag="pgaptmp_000063"
     CDS             complement(63090..63503)
                     /gene="ibpA"
                     /locus_tag="ACHKNP_RS00315"
                     /old_locus_tag="pgaptmp_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HGU4702311.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(63497..63569)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            63809..64141
                     /gene="yidQ"
                     /locus_tag="ACHKNP_RS00320"
                     /old_locus_tag="pgaptmp_000064"
     CDS             63809..64141
                     /gene="yidQ"
                     /locus_tag="ACHKNP_RS00320"
                     /old_locus_tag="pgaptmp_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU4702312.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(64143..65357)
                     /gene="yidR"
                     /locus_tag="ACHKNP_RS00325"
                     /old_locus_tag="pgaptmp_000065"
     CDS             complement(64143..65357)
                     /gene="yidR"
                     /locus_tag="ACHKNP_RS00325"
                     /old_locus_tag="pgaptmp_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HGU4702313.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYMPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAEVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            65458..66522
                     /gene="cbrA"
                     /locus_tag="ACHKNP_RS00330"
                     /old_locus_tag="pgaptmp_000066"
     CDS             65458..66522
                     /gene="cbrA"
                     /locus_tag="ACHKNP_RS00330"
                     /old_locus_tag="pgaptmp_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HGU4702314.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEIYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKRIMRSGMAHIPQLKD"
     gene            complement(66519..67856)
                     /gene="dgoT"
                     /locus_tag="ACHKNP_RS00335"
                     /old_locus_tag="pgaptmp_000067"
     CDS             complement(66519..67856)
                     /gene="dgoT"
                     /locus_tag="ACHKNP_RS00335"
                     /old_locus_tag="pgaptmp_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4702315.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(67931..69079)
                     /gene="dgoD"
                     /locus_tag="ACHKNP_RS00340"
                     /old_locus_tag="pgaptmp_000068"
     CDS             complement(67931..69079)
                     /gene="dgoD"
                     /locus_tag="ACHKNP_RS00340"
                     /old_locus_tag="pgaptmp_000068"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HGU4702316.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(69076..69693)
                     /gene="dgoA"
                     /locus_tag="ACHKNP_RS00345"
                     /old_locus_tag="pgaptmp_000069"
     CDS             complement(69076..69693)
                     /gene="dgoA"
                     /locus_tag="ACHKNP_RS00345"
                     /old_locus_tag="pgaptmp_000069"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HGU4702317.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(69677..70555)
                     /gene="dgoK"
                     /locus_tag="ACHKNP_RS00350"
                     /old_locus_tag="pgaptmp_000070"
     CDS             complement(69677..70555)
                     /gene="dgoK"
                     /locus_tag="ACHKNP_RS00350"
                     /old_locus_tag="pgaptmp_000070"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HGU4702318.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(70552..71241)
                     /gene="dgoR"
                     /locus_tag="ACHKNP_RS00355"
                     /old_locus_tag="pgaptmp_000071"
     CDS             complement(70552..71241)
                     /gene="dgoR"
                     /locus_tag="ACHKNP_RS00355"
                     /old_locus_tag="pgaptmp_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HGU4702319.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            71519..72175
                     /gene="yidX"
                     /locus_tag="ACHKNP_RS00360"
                     /old_locus_tag="pgaptmp_000072"
     CDS             71519..72175
                     /gene="yidX"
                     /locus_tag="ACHKNP_RS00360"
                     /old_locus_tag="pgaptmp_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HGU4702320.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYHWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(72221..73033)
                     /gene="yidA"
                     /locus_tag="ACHKNP_RS00365"
                     /old_locus_tag="pgaptmp_000073"
     CDS             complement(72221..73033)
                     /gene="yidA"
                     /locus_tag="ACHKNP_RS00365"
                     /old_locus_tag="pgaptmp_000073"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGU4702321.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(73148..73546)
                     /gene="yidB"
                     /locus_tag="ACHKNP_RS00370"
                     /old_locus_tag="pgaptmp_000074"
     CDS             complement(73148..73546)
                     /gene="yidB"
                     /locus_tag="ACHKNP_RS00370"
                     /old_locus_tag="pgaptmp_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HGU4702322.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(73786..76200)
                     /gene="gyrB"
                     /locus_tag="ACHKNP_RS00375"
                     /old_locus_tag="pgaptmp_000075"
     CDS             complement(73786..76200)
                     /gene="gyrB"
                     /locus_tag="ACHKNP_RS00375"
                     /old_locus_tag="pgaptmp_000075"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGU4702323.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(76229..77302)
                     /gene="recF"
                     /locus_tag="ACHKNP_RS00380"
                     /old_locus_tag="pgaptmp_000076"
     CDS             complement(76229..77302)
                     /gene="recF"
                     /locus_tag="ACHKNP_RS00380"
                     /old_locus_tag="pgaptmp_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGU4702324.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(77302..78402)
                     /gene="dnaN"
                     /locus_tag="ACHKNP_RS00385"
                     /old_locus_tag="pgaptmp_000077"
     CDS             complement(77302..78402)
                     /gene="dnaN"
                     /locus_tag="ACHKNP_RS00385"
                     /old_locus_tag="pgaptmp_000077"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGU4702325.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(78407..79810)
                     /gene="dnaA"
                     /locus_tag="ACHKNP_RS00390"
                     /old_locus_tag="pgaptmp_000078"
     CDS             complement(78407..79810)
                     /gene="dnaA"
                     /locus_tag="ACHKNP_RS00390"
                     /old_locus_tag="pgaptmp_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGU4702326.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNIPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            80107..80187
                     /locus_tag="ACHKNP_RS00395"
                     /old_locus_tag="pgaptmp_000079"
     CDS             80107..80187
                     /locus_tag="ACHKNP_RS00395"
                     /old_locus_tag="pgaptmp_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702327.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            80417..80557
                     /gene="rpmH"
                     /locus_tag="ACHKNP_RS00400"
                     /old_locus_tag="pgaptmp_000080"
     CDS             80417..80557
                     /gene="rpmH"
                     /locus_tag="ACHKNP_RS00400"
                     /old_locus_tag="pgaptmp_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGU4702328.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            80574..80933
                     /gene="rnpA"
                     /locus_tag="ACHKNP_RS00405"
                     /old_locus_tag="pgaptmp_000081"
     CDS             80574..80933
                     /gene="rnpA"
                     /locus_tag="ACHKNP_RS00405"
                     /old_locus_tag="pgaptmp_000081"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGU4702329.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            80897..81154
                     /gene="yidD"
                     /locus_tag="ACHKNP_RS00410"
                     /old_locus_tag="pgaptmp_000082"
     CDS             80897..81154
                     /gene="yidD"
                     /locus_tag="ACHKNP_RS00410"
                     /old_locus_tag="pgaptmp_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGU4702330.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            81157..82803
                     /gene="yidC"
                     /locus_tag="ACHKNP_RS00415"
                     /old_locus_tag="pgaptmp_000083"
     CDS             81157..82803
                     /gene="yidC"
                     /locus_tag="ACHKNP_RS00415"
                     /old_locus_tag="pgaptmp_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGU4702331.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            82909..84273
                     /gene="mnmE"
                     /locus_tag="ACHKNP_RS00420"
                     /old_locus_tag="pgaptmp_000084"
     CDS             82909..84273
                     /gene="mnmE"
                     /locus_tag="ACHKNP_RS00420"
                     /old_locus_tag="pgaptmp_000084"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGU4702332.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLYDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            84516..84590
                     /gene="tnaC"
                     /locus_tag="ACHKNP_RS00425"
                     /old_locus_tag="pgaptmp_000085"
     CDS             84516..84590
                     /gene="tnaC"
                     /locus_tag="ACHKNP_RS00425"
                     /old_locus_tag="pgaptmp_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HGU4702333.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            84811..86226
                     /gene="tnaA"
                     /locus_tag="ACHKNP_RS00430"
                     /old_locus_tag="pgaptmp_000086"
     CDS             84811..86226
                     /gene="tnaA"
                     /locus_tag="ACHKNP_RS00430"
                     /old_locus_tag="pgaptmp_000086"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HGU4702334.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMSLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            86317..87564
                     /gene="tnaB"
                     /locus_tag="ACHKNP_RS00435"
                     /old_locus_tag="pgaptmp_000087"
     CDS             86317..87564
                     /gene="tnaB"
                     /locus_tag="ACHKNP_RS00435"
                     /old_locus_tag="pgaptmp_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HGU4702335.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            87696..88871
                     /gene="mdtL"
                     /locus_tag="ACHKNP_RS00440"
                     /old_locus_tag="pgaptmp_000088"
     CDS             87696..88871
                     /gene="mdtL"
                     /locus_tag="ACHKNP_RS00440"
                     /old_locus_tag="pgaptmp_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HGU4702336.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            88846..89805
                     /gene="yidZ"
                     /locus_tag="ACHKNP_RS00445"
                     /old_locus_tag="pgaptmp_000089"
     CDS             88846..89805
                     /gene="yidZ"
                     /locus_tag="ACHKNP_RS00445"
                     /old_locus_tag="pgaptmp_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HGU4702337.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            89962..90711
                     /gene="yieE"
                     /locus_tag="ACHKNP_RS00450"
                     /old_locus_tag="pgaptmp_000090"
     CDS             89962..90711
                     /gene="yieE"
                     /locus_tag="ACHKNP_RS00450"
                     /old_locus_tag="pgaptmp_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HGU4702338.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            90733..91299
                     /gene="yieF"
                     /locus_tag="ACHKNP_RS00455"
                     /old_locus_tag="pgaptmp_000091"
                     /gene_synonym="chrR"
     CDS             90733..91299
                     /gene="yieF"
                     /locus_tag="ACHKNP_RS00455"
                     /old_locus_tag="pgaptmp_000091"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HGU4702339.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(91353..92690)
                     /gene="adeP"
                     /locus_tag="ACHKNP_RS00460"
                     /old_locus_tag="pgaptmp_000092"
     CDS             complement(91353..92690)
                     /gene="adeP"
                     /locus_tag="ACHKNP_RS00460"
                     /old_locus_tag="pgaptmp_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HGU4702340.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            92857..93522
                     /gene="yieH"
                     /locus_tag="ACHKNP_RS00465"
                     /old_locus_tag="pgaptmp_000093"
     CDS             92857..93522
                     /gene="yieH"
                     /locus_tag="ACHKNP_RS00465"
                     /old_locus_tag="pgaptmp_000093"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HGU4702341.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            93589..94062
                     /locus_tag="ACHKNP_RS00470"
                     /old_locus_tag="pgaptmp_000094"
     CDS             93589..94062
                     /locus_tag="ACHKNP_RS00470"
                     /old_locus_tag="pgaptmp_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702342.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            94111..94698
                     /gene="cbrC"
                     /locus_tag="ACHKNP_RS00475"
                     /old_locus_tag="pgaptmp_000095"
     CDS             94111..94698
                     /gene="cbrC"
                     /locus_tag="ACHKNP_RS00475"
                     /old_locus_tag="pgaptmp_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HGU4702343.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(94760..95482)
                     /gene="yieK"
                     /locus_tag="ACHKNP_RS00480"
                     /old_locus_tag="pgaptmp_000096"
     CDS             complement(94760..95482)
                     /gene="yieK"
                     /locus_tag="ACHKNP_RS00480"
                     /old_locus_tag="pgaptmp_000096"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGU4702344.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALD"
     gene            complement(95497..96666)
                     /gene="yieL"
                     /locus_tag="ACHKNP_RS00485"
                     /old_locus_tag="pgaptmp_000097"
     CDS             complement(95497..96666)
                     /gene="yieL"
                     /locus_tag="ACHKNP_RS00485"
                     /old_locus_tag="pgaptmp_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4702345.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(96693..98309)
                     /gene="bglH"
                     /locus_tag="ACHKNP_RS00490"
                     /old_locus_tag="pgaptmp_000098"
     CDS             complement(96693..98309)
                     /gene="bglH"
                     /locus_tag="ACHKNP_RS00490"
                     /old_locus_tag="pgaptmp_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HGU4702346.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(98395..99789)
                     /gene="bglB"
                     /locus_tag="ACHKNP_RS00495"
                     /old_locus_tag="pgaptmp_000099"
     CDS             complement(98395..99789)
                     /gene="bglB"
                     /locus_tag="ACHKNP_RS00495"
                     /old_locus_tag="pgaptmp_000099"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HGU4702347.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(99808..101685)
                     /gene="bglF"
                     /locus_tag="ACHKNP_RS00500"
                     /old_locus_tag="pgaptmp_000100"
     CDS             complement(99808..101685)
                     /gene="bglF"
                     /locus_tag="ACHKNP_RS00500"
                     /old_locus_tag="pgaptmp_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HGU4702348.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(101819..102655)
                     /gene="bglG"
                     /locus_tag="ACHKNP_RS00505"
                     /old_locus_tag="pgaptmp_000101"
     CDS             complement(101819..102655)
                     /gene="bglG"
                     /locus_tag="ACHKNP_RS00505"
                     /old_locus_tag="pgaptmp_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HGU4702349.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISSAEIMFLAINIERVRK
                     EH"
     gene            complement(102941..103666)
                     /gene="phoU"
                     /locus_tag="ACHKNP_RS00510"
                     /old_locus_tag="pgaptmp_000102"
     CDS             complement(102941..103666)
                     /gene="phoU"
                     /locus_tag="ACHKNP_RS00510"
                     /old_locus_tag="pgaptmp_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGU4702350.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(103681..104454)
                     /gene="pstB"
                     /locus_tag="ACHKNP_RS00515"
                     /old_locus_tag="pgaptmp_000103"
     CDS             complement(103681..104454)
                     /gene="pstB"
                     /locus_tag="ACHKNP_RS00515"
                     /old_locus_tag="pgaptmp_000103"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGU4702351.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(104545..105435)
                     /gene="pstA"
                     /locus_tag="ACHKNP_RS00520"
                     /old_locus_tag="pgaptmp_000104"
     CDS             complement(104545..105435)
                     /gene="pstA"
                     /locus_tag="ACHKNP_RS00520"
                     /old_locus_tag="pgaptmp_000104"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGU4702352.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(105435..106394)
                     /gene="pstC"
                     /locus_tag="ACHKNP_RS00525"
                     /old_locus_tag="pgaptmp_000105"
     CDS             complement(105435..106394)
                     /gene="pstC"
                     /locus_tag="ACHKNP_RS00525"
                     /old_locus_tag="pgaptmp_000105"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HGU4702353.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(106481..107521)
                     /gene="pstS"
                     /locus_tag="ACHKNP_RS00530"
                     /old_locus_tag="pgaptmp_000106"
     CDS             complement(106481..107521)
                     /gene="pstS"
                     /locus_tag="ACHKNP_RS00530"
                     /old_locus_tag="pgaptmp_000106"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGU4702354.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(107769..108842)
                     /gene="lpfD-O113"
                     /locus_tag="ACHKNP_RS00535"
                     /old_locus_tag="pgaptmp_000107"
     CDS             complement(107769..108842)
                     /gene="lpfD-O113"
                     /locus_tag="ACHKNP_RS00535"
                     /old_locus_tag="pgaptmp_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HGU4702355.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPSPGQFTATATITVEIVR"
     gene            complement(108853..111375)
                     /gene="lpfC-O113"
                     /locus_tag="ACHKNP_RS00540"
                     /old_locus_tag="pgaptmp_000108"
     CDS             complement(108853..111375)
                     /gene="lpfC-O113"
                     /locus_tag="ACHKNP_RS00540"
                     /old_locus_tag="pgaptmp_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HGU4702356.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVST
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(111400..112131)
                     /gene="lpfB-O113"
                     /locus_tag="ACHKNP_RS00545"
                     /old_locus_tag="pgaptmp_000109"
     CDS             complement(111400..112131)
                     /gene="lpfB-O113"
                     /locus_tag="ACHKNP_RS00545"
                     /old_locus_tag="pgaptmp_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HGU4702357.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(112179..>112733)
                     /gene="lpfA-O113"
                     /locus_tag="ACHKNP_RS00550"
                     /old_locus_tag="pgaptmp_000110"
     CDS             complement(112179..>112733)
                     /gene="lpfA-O113"
                     /locus_tag="ACHKNP_RS00550"
                     /old_locus_tag="pgaptmp_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HGU4702358.1"
                     /translation="GGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSDTADQIV
                     PLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDLKVGTPL
                     TAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEMKFIARY
                     VATNATVTAGTANADSQFTVEYKK"
BASE COUNT        28039 a        29735 c        27728 g        27231 t
ORIGIN      
        1 cttgaattat attgttttgc taaatcagaa aaatataatt tgtctttatc actgatataa
       61 tctgtgaaaa catgaaattc acaagagatt tctctattgt ttaatagaat agatgcgatg
      121 gctacaccac atccaaaaag aaaatttctg tcaataccaa atgctatatc aagagtattt
      181 ttagatctta tattttgata atgaaaatta tactcaaaaa ggatgatgtc actatcattg
      241 agttgagaca tatttattct gtatccaaaa ttttggcaaa aataagttac gggttacaac
      301 gattttcgaa tcgttctttc cctgattttt gtggcttttg cttctgcttg cgacagcagt
      361 ccttgttcct gcttgagaat gtctttaagc ggggcggcaa aatacacttt cataaaccgc
      421 cagatatccc gctgagtcag gccgatattc atcgaagaaa aataaagccc aataagatct
      481 ttatcccgcc aacgacgtgg aacgcgcgtg cgaagctgcg cccggtgcag gtcaattacc
      541 gaaattttta actcctcttc cttaccggaa aaaggcaagt gcagcaggaa atgacagata
      601 taacagtcac ggtggttaat gcccgcagca tgcatatcgc gcaccatcgt cgcgacacgc
      661 ttaataagca tacgctttac gcgaacatct ggagggttag tcgcccagtc agcacagtaa
      721 tcttccagac ttatggttgg tgtcagatct tcggtaataa taaatgaagt tctggtcagc
      781 ggattcatgc ctttttcgcc aaatgccacc ccatgcatag tatcaacgcc gacatcccgc
      841 agtcgatgaa tcgcattcca ttcgcggtct gcgcctaata ctggcatccg caatgagagt
      901 aaatttttga ttatctcttt cagggtcgtg ccgcgatgcc atttgagaaa atagcttttg
      961 cccgccattt caaagcgcag agtacggcga gtttccagtt cacgaaatac ctcaccctgc
     1021 aaggttttaa cttcctcaaa aggatctttg ccgcgccata acgtggcaaa cggctcttta
     1081 agttcaacca tccagaccac ccgttatgat atccgccgct ttctctggca gactgtataa
     1141 atcttgtgta tcagcataat atcgcgcatt ttccgcccag gcctggcgca atgaagattg
     1201 cgttaacgct ttgcgtaaaa tctcattcaa tgtttcctgg cggaatggct cagcaatagc
     1261 ctcgccgcaa ttagcctcga caatataagg cgcatagcca caaacggctg ttgttagtac
     1321 cggtaatcct gcagtaatcg cttccagcag cacaattccc gccgcttcct ggtaggcagg
     1381 atgcagtaat aaatccgccg ccgccattaa ttccgagaca tcattgcgcc ccgagaaaaa
     1441 gtgaacatta ctgcacacgc cgcgtttttc tgccagcgcc tcaaattttc gcggtttatc
     1501 ctgcccaaca acatatagca atgtgttgtg gcgcagcgaa tccggtaacg aagcaagtgc
     1561 ttcaatggaa cgatcgacac ctttacgcgt gaagtctgaa ccgacctgca acaataaata
     1621 ttgttgttcg gttattccat tcttcttacg gaagatttca cggctattgg ctggctgctg
     1681 gctatattta cgatcaggat aaatccccgg tggcagaata tgaaaacgct ccgcttcagt
     1741 ctgataatgt ttctggaaat cggcgatttg cttatctgtc agcatcagca gctgtgtcgg
     1801 cttaccctgt tcgaaggttg cccgctcaaa ggcggcataa tggcgataac gtgacgtcag
     1861 gcgatagaaa aagccttttt cctgcgctac tttctcggca taacaaacat cagcggcata
     1921 ataaacgtcc agccccggca ttttgttgaa tccaacgact ttatcgacgg gatgttcgcg
     1981 taaatgtttt tgcacccagg caaaatactc cgcattgcgc ccgtgattgg tatgcgattt
     2041 aaccggcact ttgatcagtt caaatacatc agggcattcg ccttcccacg actgggtata
     2101 aacccgaaca tgatgacctc gggcggcgac tgtctgagca atacgcataa aatcacgctg
     2161 caaaccgcca aagggaaaat atttatataa acaaaaagca acgatcataa cgacgtacct
     2221 gtcgtggagg agggcagtaa tttatcgaca gcagcaatga catctgccgc cggaataacc
     2281 gaaagataca tctcatttcg gtcacgctga tcgcgcgttg gcatttcccg gtaatctccc
     2341 gcccagaatt gaatcatgtt atttgaccag ggacgccaga aaatatgatc tgtcgcacca
     2401 aacagcgata tcagcggcgt attaactgca gcggcaatat gcgccggtgc ggaatcaacg
     2461 ccaataaaca gctgcgcatg atcgattaac gcaccaagtt ccgggaaggt cacctttcca
     2521 gccagcgccg ttactggtgg cgtctggcat ccctgcgcaa tttcattgac gcaggccaga
     2581 tcgtctttat ctgggccgga cgtcagaacg acttcataac cacgagcatg taaggcatca
     2641 atcacagcgg aaaacttggc gttgtcccag catttgaaga tttgccgcgc cgtaggttgg
     2701 ataaccacat aattttgtcc aacaccagcg tgatcaagtt cgcgacgcat acgtttccag
     2761 cttgcaggcg ggtaactcat ggttgtctgc ttcaccaacg aatcaactcc caatggggtc
     2821 agcacggata agttactttc caccacattt ccaccctgca acggcaccaa atgggtgaaa
     2881 cttttacgcc aaaaagcaga ctgccgatga tgataatcct gggaaatttt cacacgggca
     2941 tttaataagc gaaccagtat agcaaccatc cattgatcgg tgagattgac gataaggtca
     3001 tacttattgg cacgtaatac cttgatgaga tgaaaaaagt tggcaatttt ttctgaggct
     3061 tttgcttttt tattttttat gccgtagagc gcgttaatct ctggattttc agacaggatc
     3121 gggatggtgt cctgataaag cagcacatcg atttttgcgt cagggtaatt ttttttcagc
     3181 gaactaatga cgggagtagt taataacata tccccatgaa aacgcatctt aatgagcaaa
     3241 attcttcgaa atggcttatc cacaagtaac tctttttgtt gagattatcc gattaaggtg
     3301 aagcagaaaa agcacgctac cgccccaggc tcaacagcta cctgaatact gataccgcac
     3361 tgatattgac gcgctagtgt atcacttctt tcgcggcaat ccgacccgaa tgatttttaa
     3421 gcacattgtt gattttcaat aaagaaatca atcaaataaa atcattagca accatattct
     3481 ttcactgttg ctctgcgatt ccttcctgca cgtacagcag aaatcaatga atcgcataaa
     3541 aatacaaaaa aagtatagat gttcagcaag aattggcggg taaattgcta tttaagtcaa
     3601 aaacgggaaa agtaatggta aagccacagc taaatacata gaatccccag cacatccata
     3661 agtcagctat ttactatgct cgaattgctt tacaccgccc ttctctacct tattcagccg
     3721 ctgatctgga tacggctctg ggtgcgcgga cgtaaggctc cggcctatcg aaaacgctgg
     3781 ggtgaacgtt acggttttta ccgccatccg ctaaaaccag gcggcattat gctgcactcc
     3841 gtctccgtcg gtgaaactct ggcggcgatc ccgttggtga gcgcgctgcg ccatcgttat
     3901 cctgatttac cgattaccgt tacaaccatg acgccaaccg gttcggagcg cgtacaatcg
     3961 gctttcggga aggatgttca gcacgtttat ctgccgtatg acctgcccga tgcgctcaac
     4021 cgtttcctga ataaagtcga ccccaaactg gtgttgatta tggaaaccga actatggcct
     4081 aacctgattg cggcgctaca caaacgtaaa attccgctgg tgatcgctaa cgcgcgactc
     4141 tctgcccgct cggccgcagg ttatgccaaa ctgggtaaat tcgtccgtcg cttgctgcgt
     4201 cgtattacgc tgattgctgc gcaaaatgaa gaagatggtg cacgttttgt ggcgctgggc
     4261 gcaaaaaata atcaggtgac cgttaccggt agcctgaaat tcgatatttc tgtaacgccg
     4321 cagttggctg ctaaagccgt gacgctgcgc cgccagtggg caccacaccg cccggtatgg
     4381 attgccacca gcactcacga aggcgaagag agtgtggtga tcgccgcaca tcaggcattg
     4441 ttacagcaat tcccgaattt attgctcatc ctggtacccc gtcatccaga acgcttcccg
     4501 gatgcgatta atcttgtccg ccaggctgga ctaagctata tcacacgctc ttcaggggaa
     4561 gtcccctcca ccagcacgca ggttgtggtt ggcgatacga tgggcgagtt gatgttactg
     4621 tacggcattg ccgatctcgc ctttgttggc ggttcactgg ttgaacgtgg tgggcataat
     4681 ccgctggaag ctgccgcaca cgctattccg gtattgatgg ggccgcatac ttttaacttt
     4741 aaagacattt gcgcgcggct ggagcaggca agcgggctga ttaccgttac cgatgccact
     4801 acgcttgcaa aagaggtgtc ctctttactc accgacgccg attaccgtag tttctatggc
     4861 cgtcatgccg ttgaagtact gtatcaaaac cagggcgcgc tacagcgtct gcttcaactg
     4921 ctggaacctt acctgccacc gaaaacgcat tgaggttgtt atgcaaaaac gggcgattta
     4981 tccgggtact ttcgatccca ttaccaatgg tcatatcgat atcgtgacgc gcgccacgca
     5041 aatgttcgat cacgttattc tggcgattgc cgccagcccc agtaaaaaac cgatgtttac
     5101 cctggaagag cgtgtggcgc tggcacagca ggcaaccgcg catctgggga acgtggaagt
     5161 ggtcgggttt agtgatttga tggcgaactt cgcccgtaat caacacgcta cggtgctgat
     5221 tcgtggcctg cgtgcggtgg cggattttga atatgaaatg cagctggcgc atatgaatcg
     5281 ccacttaatg ccggaactgg aaagtgtgtt tctgatgccg tcgaaagagt ggtcgtttat
     5341 ctcttcatcg ttggtgaaag aggtggcgcg ccatcagggc gatgtcaccc atttcctgcc
     5401 ggagaatgtc catcaggcgc tgatggcgaa gttagcgtag cgtttatgcc ggatggtatg
     5461 ccatccggcg cgcatgaatt acttctggca ctgccgacaa taaaacgttg cccgctgcgc
     5521 atgtttagtc gccacaatcg gcgtaccgca cacccgacac ggctcgcctt ttcgcccgta
     5581 aacctgcaat tcctgtgcga aatagcccgg tttaccatca ctttgcagaa aatctttcag
     5641 cgttgtaccg ccctgctcaa tcgaacgcag caacaccgct ttaatcaccc gagctaacaa
     5701 ttcacactct gccagcgaca gtgatgacgc cagccgatcc ggatggatcc ccgccgcaaa
     5761 cagtgattcg ctggcataga tattccctac ccctaccacc agcttgttat ccatcagcca
     5821 cggtttaatc gccgtttttt tcttcgcgca cttctgatgc agatactcac cattgaaatc
     5881 gtcgctgagc ggctccggtc caagatgggc cagcacatta tgcccttcca gctctttggt
     5941 ccacagccag gcaccaaagc ggcgcggatc ggtgtagcgc agcactttac cgttgctcat
     6001 caccaaatcc acatggtcat gcttttcagg gggaagttct tctggaagga tgcgcaggct
     6061 gccagacatc cccaaatgaa tgataatcca gccctcaggc agctccagca gcaaatattt
     6121 agcccgccgc tgcacgctaa gcactggttg gtcgcttaaa cggtagatct cttctgaaac
     6181 cggccagcgc aagcgtccgt tgcgcaccac tgcatgaaga atggttgcac caacgagatg
     6241 cggttctatg ccgcgacggc tggtttcaac ttcgggtaat tcaggcatag catctccagg
     6301 aatgaacaga tgcaatcaat atgggggcaa gcagataaca aaaaaccccg ccggagcgag
     6361 gttttttgtt acatcaaagc gagaattatt tgattttcgc ttctttgtag atcacgtgct
     6421 gacgaacaac tggatcgaat tttttcagtt ccagtttttc cggcttagta cgtttgttct
     6481 tcgtagtggt atagaagtga ccagtaccag cagaagaaac cagcttgatt ttctcacgaa
     6541 tacctttagc catgatttat ttcctctaag tacttagtac ttttcgccac gggcacgcag
     6601 ttcagccaga actgtatcga tgcctttttt atcgattaca cgcatacctt tagcagatac
     6661 gcgcagggtg acaaaacgct tctcgctctc aacccagaaa cggtgagagt gcaggttcgg
     6721 caggaaacgg cgtttagtcg cgttcagtgc gtgggaacgg ttgttaccgg tcaccggacg
     6781 cttgccagta acttggcaga ctcgggacat gtctattctc caaaaatcaa attagctcga
     6841 gcttcgtatg gggtattggc gcctcgtcag gctttacagc ccggtcatcg cagttctatg
     6901 tgaactctcg attgccaggc ccaaatgcca aacccgagat tctcaaaggt ggcgtagtat
     6961 acgctgactc agcgatgtgc tcaagtcccg aacagacaaa gatcccgaag gatcgcgcat
     7021 agcgggttaa atccagccgc gttcggcaaa agaaacatac tctccacgcc caatcacgat
     7081 atggtcgagc acgcgtaaat ccatgaactg acaactcttt attatccgtt cagtaatgag
     7141 tttatccgct ttactgggtt cagcacaacc cgaagggtga ttatgtgcaa ggatcagcgc
     7201 cgaggcgttt atttttatcg cttcgcggat aatttcccga ggatggactt caacatggtt
     7261 tagcgtgccg gaaaaaagac ggctatgcgt tataacccgg tgttgtgagt cgagaaagat
     7321 caccataaag atctcccgct cctcacccgt gagctggctt tgtaaaaatt cccgcgtcat
     7381 ctccgggctg agtaaagggc tttcttcacg catccgcacg ttgtagtaac gccgcgccag
     7441 ttcagcaatc ccctttaact gggcaaattt cgctacgcca attccatgaa cgccactaaa
     7501 ttgttcatat tcagaggtta acaagccata aagagagccg aaattctcca gcatctcttt
     7561 tgccagggtt aatacatctt taccgcgcgt tccggtacgc agaaataacg ccagcagctc
     7621 gacatccgtt aaggcgctaa taccaaactt cagcattttt tcgcgcggca tcaacagctg
     7681 tgcattgttt ttcaccttca cctcctttgt ggtgctcgca tcctgtcata gccgttccgt
     7741 taaatcgacg gccagttttc aatcctggaa agcgcctcgc aaagtgattc tcaggtgaag
     7801 gcacgacaac aaaagtattg tgataaaatc gccaacttct ggtgtcaccc aacaggaaaa
     7861 atcatcatga gcctggccgg taaaaaaatc gttctcggcg ttagcggcgg tattgctgcc
     7921 tataaaaccc ctgaactggt gcgtcgtttg cgcgatcgcg gggccgacgt ccgcgtagcc
     7981 atgaccgaag cggcaaaagc ctttatcacc ccacttagct tgcaggcggt ttctggttat
     8041 cccgtttccg acagtttgct ggacccggca gccgaagccg ctatgggcca tattgagctg
     8101 ggtaaatggg ctgatttagt gattctcgcc cctgccacgg cagatttgat tgcccgtgtt
     8161 gctgccggaa tggcgaatga cctggtatcg acgatttgtc tggctacacc tgcgcctgta
     8221 gccgtgctcc ccgccatgaa ccagcagatg taccgtgccg ctgccacgca gcataattta
     8281 gaggtgcttg cttcccgtgg tttgctcatc tgggggccag acagtggcag tcaggcttgt
     8341 ggtgatatcg gtcctgggcg aatgctcgat ccgttaacca ttgtggatat ggcggtggcg
     8401 catttttcgc ccgtcaacga cctgaaacat ctgaacatta tgattaccgc cggtccgacg
     8461 cgtgaaccgc tcgatccggt gcgttatatc tctaatcaca gctccggcaa gatgggtttt
     8521 gctatcgccg ccgccgctgc ccgtcgtggc gcgaacgtca cgctggtatc aggtccggtt
     8581 tcactaccga cgccaccgtt tgttaaacgt gttgatgtga tgactgcgct ggaaatggaa
     8641 gccgccgtga atgcttctgt acagcagcaa aatattttta tcggctgcgc cgccgtggcg
     8701 gattatcgcg cagctaccgt ggccccagag aaaatcaaaa agcaggccac gcagggtgat
     8761 gaattaacaa taaaaatggt taaaaacccc gatatcgtcg caggcgttgc cgcactaaaa
     8821 gaccatcgac cctacgtcgt tggatttgcc gccgaaacaa ataatgtgga agaatacgcc
     8881 cggcaaaaac gtatccgtaa aaaccttgat ctgatctgcg cgaacgatgt ttcccagcca
     8941 actcaaggat ttaacagcga caacaacgca ttacaccttt tctggcagga cggagataaa
     9001 gtcttaccgc ttgagcgcaa agagctcctt ggccaattat tactcgacga gatcgtgacc
     9061 cgttatgatg aaaaaaatcg acgttaagat tctggacccg cgcgttggga aggaatttcc
     9121 gctcccgact tatgccacct ctggctctgc cggacttgac ctgcgtgcct gtctcgacga
     9181 cgccgtagaa ctggctccgg gtgacactac gctggttccg accgggctgg cgattcatat
     9241 tgccgatcct tcactggcgg caatgatgct gccgcgctcc ggattgggac ataagcacgg
     9301 tatcgtgctt ggtaacctgg taggattgat cgattctgac tatcagggcc agttgatgat
     9361 ttccgtgtgg aaccgtggtc aggacagctt caccattcaa cctggcgaac gcatcgccca
     9421 gatgattttt gttccggtag tacaggctga atttaatctg gtggaagatt tcgacgccac
     9481 cgaccgcggt gaaggcggct ttggtcactc tggtcgtcag taacacatac gcatccgaat
     9541 aacgtcataa catagccgca aacatttcgt ttgcggtcat agcgtgggtg ccgcctggca
     9601 agtgcttatt ttcaggggta ttttgtaaca tggcagaaaa acaaactgcg aaaaggaacc
     9661 gtcgcgagga aatacttcag tctctggcgc tgatgctgga atccagcgat ggaagccaac
     9721 gtatcacgac ggcaaaactg gccgcctctg tcggcgtttc cgaagcggca ctgtatcgcc
     9781 acttccccag taagacccgc atgttcgata gcctgattga gtttatcgaa gatagcctga
     9841 ttactcgcat caacctgatt ctgaaagatg agaaagacac cacagcgcgc ctgcgtctga
     9901 ttgtgttgct gcttctcggt tttggtgagc gtaatcctgg cctgacccgc atcctcactg
     9961 gtcatgcgct aatgtttgaa caggatcgcc tgcaagggcg catcaaccag ctgttcgagc
    10021 gtattgaagc gcagctgcgc caggtattgc gtgaaaagag aatgcgtgag ggtgaaggtt
    10081 acaccaccga tgaaaccctg ctggcaagcc agatcctggc cttctgtgaa ggtatgctgt
    10141 cacgttttgt ccgcagcgaa tttaaatacc gcccgacgga tgattttgac gcccgctggc
    10201 cgctaattgc ggcacagttg cagtaatatg acgccggatg acttttcatc cggcgagttt
    10261 ctttaaacgc caaactcttc gcgataggcc ttaaccgccg ccagatgttc cgccatttcc
    10321 ggcttctctt ccaggtaagc aatcaggtct ttcagggtga tgatagagat cactttgcag
    10381 ttgtaatcac gctcaacttc ctgaatcgcc gaaatctcgc cgcgcccccg ttcctgacga
    10441 tcaagcgaaa tcaacacgcc agcaagcgtc gcgccattag cctgaataat ctccatcgat
    10501 tcgcgaatcg ccgttccggc ggtgatcaca tcatctacca gcattacgcg tccttgtaac
    10561 gcgctaccaa ccagattgcc gccttcaccg tggtcttttg cctctttgcg gttaaagcag
    10621 tacggcaggt caaggtcatg atgctccgcc agcgccacgg cggtagtggt ggcaatcggg
    10681 atccctttgt aagcagggcc aaacagcaga tcgaactcaa ggccggaatc caccaacgct
    10741 tcagcgtaaa aacggcctaa cagtgccaga tcgcgcccgg tattaaacag cccggcgttg
    10801 aagaaatagg ggcttttgcg cccggacttc aacgtaaact cgccaaactt taacacctgc
    10861 ttgccaagcg caaattcaat aaactggcgc tgatatggtt tcatgccttc gctcctcatc
    10921 ttacttttct acagacaaaa aaaaggcgac tcatcagtcg ccttaaaaat cagtttgcca
    10981 gcgccgcctt ctgcgtcgct acaatggatt cgattccccc tcgggccagc gccaacaagg
    11041 tgagtagctc ttcatgggtg aacggctcgc cttctgccgt cccctgcact tcaatgatgc
    11101 gcccgtcttc ggtcatcact acgttcatgt cggtctctgc ggctgagtct tcaacgtatt
    11161 ccagatcgca aattgcttca ccgtttacaa taccgacaga aactgcggct accatccctt
    11221 tcatcgggtt ggttttcagc ttgccgtttt ccaccagctt ctgtagcgca tctgccagcg
    11281 ccacgcaggc acccgtaatc gacgcggtac gcgtgccacc atcagcctga agcacgtcgc
    11341 agtccagcgt aatggtgaac tcacccagcg ctttcaaatc tactgccgcg cgaagagcac
    11401 gggcgatcag acgctggatt tccattgtgc gtccaccctg cttacctttc gccgcttcac
    11461 gagcgttacg ggtgtgggta gaacgtggca gcatgccgta ctctgcggtg atccagccct
    11521 ggccctgacc tttcaggaag cgcggcacgc cttcttcaat agaggcggta cacaacactt
    11581 tggtatcgcc aaattcgacc agcaccgagc cttctgcatg ttttgtatag ttacgagtca
    11641 gggtaacggg acgcacctga ttattgctac ggcctgctgg acgcatattg aaatctccgg
    11701 cttgaaacaa atgtggctgc gcattatacg gacttccggc ggttattcct atcctgacaa
    11761 ggcatcgatg gctataatcc ttccacctct ccttttataa acaggaacgt ctatgatccg
    11821 cagtatgacc gcctacgccc ggcgtgaaat caagggtgaa tgggggagcg caacctggga
    11881 aatgcgctcg gtaaaccagc gttatctgga aacttacttt cgtctgccgg agcagttccg
    11941 tagccttgaa cctgtcgttc gcgagcgtat tcgttctcgc ctgacgcgcg gtaaagtgga
    12001 atgtaccctg cgctatgagc cagatgttag cgcgcaaggt gagctgatcc tcaacgaaaa
    12061 actggctaaa cagctggtaa ctgccgcgaa ctgggtaaaa atgcagagtg acgaagggga
    12121 aatcaacccg gttgatattc tgcgctggcc gggcgtaatg gcagcccagg agcaggatct
    12181 cgacgcgatt gccgctgaaa ttctcgcggc gttggatggt accctggacg actttatcgt
    12241 cgcacgtgaa accgaaggtc aggcgctgaa agcattgatc gagcagcgtc tggaaggcgt
    12301 caccgccgaa gtggtcaaag tccgcgccca tatgccggaa atcctgcaat ggcagcgtga
    12361 gcgtctggtc gcgaagctgg aagatgctca agtgcaactg gaaaacaacc gcctggagca
    12421 ggagttggtt ctgctggcac aacgaattga cgttgccgaa gaactggacc gcctggaagc
    12481 gcatgtcaaa gagacctaca acattctgaa gaaaaaagaa gccgttggtc gtcgtctgga
    12541 ctttatgatg caggaattca accgcgagtc gaacactctt gcgtcgaagt ctatcaatgc
    12601 cgaagtgaca aactccgcga ttgagctgaa agtgttgatt gagcagatgc gcgagcagat
    12661 tcagaatatt gagtaactcg taacactaat tcttactttt cagccttcac aaatgccgcc
    12721 cactcaaaca gagcggcatt tttcttcccc gcaacttcaa ttctgttttg cgtgcctgct
    12781 ccagattttg cgatgttttt ttgcccagca cactgagaac gtgagatact cacaactgta
    12841 tataaataca gttacagatt tactttcttt gcaatcgata tcacatggag tgggcaatga
    12901 acgaacatca tcaacctttt gaagaaatac ggcattatgg cactgaaggg caagaatttt
    12961 ggtcagctag agaattggca ccgttactgg attatcggga ctggcgcaat tttcaaaagg
    13021 tactggcccg cgcgactcaa gcgtgcgaag ccagcaatca agctgcatct gaccatttcg
    13081 ttgaaaccac caaaatggtt gtattaggtt caggggctca acgagaactt gaagatgttc
    13141 atctctcccg ctatgcctgt tacctggtag tacaaaacgg cgaccctgcg aaaccggtta
    13201 ttgcggcagg gcaaacttat tttgctatcc agacccgacg gcaggagctg gctgacgatg
    13261 aagctttcag gcaacttcgt gaagacgaaa agcgtctttt tctgagaaat gaattgaaag
    13321 aacataacaa acaattggtt gaggcagcgc agcaagctgg ggtagcgaca gctatcgatt
    13381 tcgccatatt tcagaatcat ggttaccggg ggctgtatgg tggattagat cagaaagcca
    13441 tccatcagcg gaaggggctg aaaaagagtc agaagatcct ggatcatatg ggttcaacag
    13501 aactggcggc taatctcttt cgagctaccc aaacagaaga aaaactcaag cgggatggcg
    13561 tgaattcaaa acagcaagct aataccacac actttgacgt gggtagcaag gtgaggcaaa
    13621 ccattcagga acttggcgga accatgcctg aggagttgcc gaccccgcag gtcagcatca
    13681 agcagttgga aaacagtgta aaaattacag agaagaaata gcagaaacta aaaactcttt
    13741 ttgttgattg agatacccga tgcgtaagcc aaagtccagg tgcggtgaac acaatggcta
    13801 aacattgcac ctttcttttc cacccctcaa ttttaaccta ttttttctta tgcattttct
    13861 cagacaagaa gtcagaagaa tgcatctacg ctacagaaaa tagcgatttc acataactac
    13921 aagttatcaa tttcctctca ccttaaaaaa tctcaatcgt gacaatacgc acaaatcgct
    13981 accctgccag acagattttt agggagagaa ccatgctgtt acacattttg tatttggttg
    14041 gcattactgc cgaagccatg acgggggctc tggcggccgg gcgacgccgc atggatacat
    14101 ttggcgtaat tattattgcg accgccaccg caattggcgg agggtcagtg cgcgatattc
    14161 tgctgggcca ctatccgctc ggctgggtca aacacccgga atatgtgatt atcgtcgcta
    14221 ccgccgcagt actcaccact atcgttgctc ctgtaatgcc ttacctgcgc aaagtgtttc
    14281 tggtgctcga tgcgcttgga ctggtggtct tttctatcat cggcgcacag gttgcactgg
    14341 atatggggca cggcccaatt attgccgttg tcgcggcggt aaccaccggc gtgtttggcg
    14401 gcgttttgcg cgatatgttc tgtaaacgta tcccactggt attccagaaa gagttgtatg
    14461 ccggtgtctc atttgcctcc gccgtgctgt acatcgcgct gcaacactat gtttctaacc
    14521 atgatgtggt aattatctcc accctggtat tcggcttttt tgcccgctta ctggcgctac
    14581 gccttaagct gggattaccg gtcttttact acagccacga aggacactaa ggttcaaaac
    14641 ctgtgatctg ctgggcagcc agccaactgc ccagcttctt gatttgcgca ttttccttcc
    14701 attcaataac ctgtctggcg cgccccgatc cagtccccgg cagctgctgc cagaactgct
    14761 ccgtgctaaa taaaagttgc gaccaggacc gttcatcact ggcattaagc gccgcccggg
    14821 ttagcggtat tcccattgcc atcacccagc gagtaaaagg ctgcttacga gccagattaa
    14881 accgatgcca tagctgcgca cttttacttt tcgcgatccc cggcgtgttc tgtaattgct
    14941 ctggcgttaa taaaagccag gaaaagatat gctcaaagcg atgagtctga tgcagcgcgc
    15001 gccagccggc ctcaccaata ccatccagcc ccagaacctg ttttgacccc agccagacta
    15061 agcgtgaaat gaactgttcc tgacaaacat cagaagcaaa gtagcaggtc aacgagttaa
    15121 agcggttttc tggtggtgtc ggttttgtac gttctgcacc gcgccagacc acatcatcaa
    15181 tgcgaggaat accctgaccg gcaaggctga cgaggatctg atcaccaggc gcaatatccc
    15241 actcctgcca gcgcctgacg gaaccaatat tcacccgctg gactttttta tcatccagca
    15301 tgacaggtgc gagtgacgca accaccgata ttttaccgct cttacccacc gcaaactgaa
    15361 tcgccttcac ttcggcaacc tgagctacag gttgatattt ccaggccacc agccattctg
    15421 cctggcctgg tagccaatgg cgggattcgg gttctttcgc ccctcgcaca accacgccat
    15481 cggtgacgaa gggtaatttc gctttccacc actcattgcg tacgcgcgca acctcatcag
    15541 catttttaac cgcacgggta tacctctgcg ttagagtaaa acctgcggta gccagctcct
    15601 ttaaacgatc ggtcattaac tgcggtccat ccggccatgc ccagacaaaa acgcccagag
    15661 aattcagcgt gtcgctatcg tcctggcgca tcatcaggcc agcaactttt gcgcgggcat
    15721 ttattccccc catttgttgt tggatatgcc cctcgcgctg gagaaatatt tccccctgaa
    15781 gcgtactgtt ggctaaaggc ccgctaacgg tttgcggcac agcggaaatt aagctaactt
    15841 tctgcgtcca gtcctcgcct ttcaggccat taccgcgact gattgctttg ttcagtttcc
    15901 cgtcccgata aaccagggtt accgcaacgc catcaacttt tggctgcacc caaagatcgc
    15961 tacgttctcg catccacaga cttaatgcgt ttttatccgc cattttgcgt acacccgtat
    16021 gggcaaccgg atgcataacc gcaccattta aaggcggcat catcacgtcg cggggctcgc
    16081 tcacaaagca gcgttgccac tgcgtaagac gggcgcttaa ctgatcgtaa acaccgtctt
    16141 ccacctcact ttttccttcc ttccagtagt catcgtccca ctgttttatt tgctgttgca
    16201 ggcgggaaat ttcttcctgt gctctggctg gcgaccaggc cggacagacc gcccacacag
    16261 atgattgcca gcacaagata cttattaata tcgccatcca tactttcatc ttcacctccg
    16321 ctgtagtcag gctggcagat atacaacgtg gtgaaggcaa agccgagtgg caaaaacgga
    16381 gtctgcgagg acgcttcctg agaatcgtct ttattgcagt aaatcacagg caaatgcgga
    16441 aacagctacg caaaacgcaa caactttgcg caaaaagtgt gagcaagggc tacgtcacat
    16501 ggccgcgccg tgtataataa gctcgtatgt aggctttatt tcgctaatca catacgaaag
    16561 atactcatgg ctcaaggcac gctttatatt gtttctgccc ccagtggcgc gggtaaatcc
    16621 agcctgattc aggctttatt aaaaacccaa ccgttgtatg acacccaggt ttctgtttca
    16681 cacaccacac gccaaccgcg tcctggtgaa gtccacggtg aacattattt ctttgttaat
    16741 catgatgaat ttaaagaaat gattagcaga gatgcgttcc tcgaacacgc agaagttttt
    16801 ggtaattact atggcacttc gcgtgaggcc attgagcaag tactggcgac cggtgtcgat
    16861 gtttttctcg atatcgactg gcagggcgcg cagcaaattc gccagaagat gccgcacgcg
    16921 cggagtatct ttattttacc gccgtccaaa attgaactgg accgccgtct acgcggtcgc
    16981 ggtcaggaca gcgaagaggt cattgcaaag cgtatggcgc aagctgttgc agaaatgagc
    17041 cattacgccg aatatgatta tctgattgtg aatgatgact tcgataccgc gttgaccgat
    17101 ttgaagacca ttattcgcgc cgaacgtctg cgcatgagcc gccaaaagca gcgtcatgac
    17161 gctttaatca gcaaattgtt ggcagactga acctgatttc agtatcatgc ccagtcattt
    17221 cttcacctgt ggagcttttt aagtatggca cgcgtaactg ttcaggacgc tgtagagaaa
    17281 attggtaacc gttttgacct ggtactggtc gccgcgcgtc gcgctcgtca gatgcaggta
    17341 ggcggaaagg atccgctggt accggaagaa aacgataaaa ccactgtaat cgcgctgcgc
    17401 gaaatcgaag aaggtctgat caacaaccag atcctcgacg ttcgcgaacg ccaggaacag
    17461 caagagcagg aagccgctga attacaagcc gttaccgcta ttgctgaagg tcgtcgttaa
    17521 tcacaaagcg ggtcgccctt gtatctgttt gaaagcctga atcaactgat tcaaacctac
    17581 ctgccggaag accaaatcaa gcgtctgcgg caggcgtatc tcgttgcacg tgatgctcac
    17641 gaggggcaaa cacgttcaag cggtgaaccc tatatcacgc acccggtagc ggttgcctgc
    17701 attctggccg agatgaaact cgactatgaa acgctgatgg cggcgctgct gcatgacgtg
    17761 attgaagata ctcccgccac ctaccaggat atggaacagc tttttggtaa aagcgtcgcc
    17821 gagctggtag agggggtgtc gaaacttgat aaactcaagt tccgtgataa gaaagaggcg
    17881 caggccgaaa actttcgcaa gatgattatg gcaatggtgc aggatatccg cgtcatcctc
    17941 atcaaacttg ccgaccgtac ccacaacatg cgcacgctgg gctcacttcg cccggacaaa
    18001 cgtcgccgca tcgcccgtga aactctcgaa atttacagcc cgctggcgca ccgtttaggt
    18061 atccaccaca ttaaaaccga actcgaagag ctgggttttg aggcgctgta tcccaatcgt
    18121 taccgcgtaa ttaaagaagt ggtgaaagcc gcgcgcggca accgtaaaga gatgatccaa
    18181 aaaatcctct ctgaaatcga agggcgtttg caggaagcgg gaattccgtg ccgcgtcagt
    18241 ggtcgcgaga agcatcttta ttcgatttac tgcaaaatgg tgctcaaaga gcagcgtttt
    18301 cactcaatca tggacatcta cgctttccgc gtgatcgtca atgattccga cacctgttat
    18361 cgcgtgctcg ggcaaatgca tagcctgtac aagccgcgtc cgggtcgcgt gaaagactac
    18421 atcgccattc caaaagcgaa cggctatcag tctttgcaca cctcgatgat cggcccgcac
    18481 ggtgtgccgg ttgaggtcca gatccgtacc gaagatatgg atcagatggc ggagatgggt
    18541 gttgccgcgc actgggctta taaagagcac ggcgaaacca gcaccaccgc gcaaatccgc
    18601 gcccagcgct ggatgcaaag cctgctggag ctgcaacaga gcgccggtag ttcgtttgaa
    18661 tttatcgaga gcgttaaatc cgatctcttc ccggatgaga tttacgtttt cacaccggaa
    18721 gggcgcattg tcgagctgcc tgccggtgca acgcccgtcg acttcgctta tgcagtgcat
    18781 accgatatcg gccatgcctg cgtgggcgca cgcgtcgacc gccagcctta cccgctgtcg
    18841 cagccgctta ctagcggtca gaccgttgaa atcattaccg ctccgggcgc tcgcccgaat
    18901 gccgcttggc tgaactttgt cgttagctcg aaagcgcgcg ccaaaattcg tcagttgctg
    18961 aaaaacctca agcgtgatga ttctgtaagc ctgggccgtc gtctgctcaa ccatgctttg
    19021 ggtggtagcc gtaagctcaa tgaaatcccg caggaaaata ttcagcgcga gctggatcgc
    19081 atgaagctgg caacgcttga cgatctgctg gcagaaatcg gccttggtaa cgcaatgagc
    19141 gtggtggtcg cgaaaaatct acaacatggg gacgcctcca ttccaccggc gactcaaagc
    19201 cacgggcatc tgccgatcaa aggcgctgat ggcgtgctga tcacctttgc gaaatgctgc
    19261 cgccccattc ctggcgaccc gattatcgcc cacgtcagcc ccggtaaagg tctggtgatc
    19321 caccatgaat cctgccgtaa catccgtggc taccagaaag agccagagaa gtttatggct
    19381 gtggaatggg ataaagagac ggcgcaggag ttcatcaccg aaatcaaggt ggagatgttc
    19441 aatcatcagg gtgcgctggc aaacctgacg gcggcaatta acaccacgac ctcgaatatt
    19501 caaagtttga atacggaaga gaaagatggt cgcgtctaca gcgcctttat tcgtctgacc
    19561 gctcgtgacc gtgtgcatct ggcgaatatc atgcgcaaaa tccgcgtgat gccagacgtg
    19621 attaaagtca cccgaaaccg aaattaatgt tttatgaacc caacacgtta tgcacgcatc
    19681 tgcgaaatgc tcgccaggcg gcagcctgat ctgaccgtct gcatggagca ggtccacaaa
    19741 cctcataacg tttctgcgat tattcgtacc gcagatgccg ttggcgtaca tgaagttcac
    19801 gccgtctggc ctggtagccg catgcgcacc atggcttcgg cagcggcggg tagtaacagc
    19861 tgggtacagg tgaaaacaca ccgcaccatt ggcgatgccg tcgctcatct caaaggccag
    19921 ggcatgcaga ttctggcaac ccatctttct gataacgctg tcgatttccg cgaaattgat
    19981 tacactcgcc cgacctgcat tttgatggga caggagaaaa cgggcatcac gcaggaagca
    20041 ttggccctgg cggatcagga catcatcatt ccgatgatcg gcatggtgca gtcgctgaat
    20101 gtgtccgttg cctcagccct cattctttac gaagcccagc gtcagcggca aaatgcaggc
    20161 atgtacctgc gtgaaaacag catgttgccg gaagcagagc aacaacgcct gttgtttgaa
    20221 ggcggctatc cggtgctggc gaaagtcgca aaacgcaaag gcctgcctta tccccacgtc
    20281 aatcagcaag gcgagatcga agctgatgcc gactggtggg ctactatgca ggctgcaggg
    20341 taagtgccat gaaaggtcgc ctgttagatg ctgtcccact cagttcccta acgggcgttg
    20401 gcgcagcact tagtaacaaa ctggcgaaaa tcaacctgca taccgtgcag gatctactct
    20461 tacaccttcc cctgcgctac gaagatcgca cccatctcta ccccatcgga gaactactgc
    20521 cgggcgttta tgccacggtg gaaggcgaag tgctgaactg caatatctcc ttcggcggtc
    20581 ggcggatgat gacctgccag atcagcgacg gttccggcat cctcaccatg cgctttttca
    20641 atttcagcgc ggcaatgaaa aatagcctgg cggcgggccg ccgtgtactg gcttatggcg
    20701 aagcaaagcg cggtaaatat ggtgcggaga tgatccaccc ggaataccgc gtgcagggcg
    20761 atctcagcac gccagaatta caggaaacgc tcacgccggt ttatccaaca acggaaggcg
    20821 taaagcaggc cacgctgcgt aaattaaccg accaggcgct ggatctgctc gacacctgcg
    20881 ccattgaaga actcctgccg ccggaactgt cacaaggaat gatgacgcta ccggaagcgt
    20941 tgcgcacttt gcaccgcccg ccaccgacgc tacagcttag cgatctggaa accgggcagc
    21001 atccggcgca acgtcgtctg attctggaag aactgctggc gcacaacctc agcatgttag
    21061 ccttacgtgc cggagcacag cgttttcatg cccagccgct gagcgccaat gacacgctga
    21121 aaaataaact cctcgccgcc ttaccgttca agccaacggg cgcacaggca cgcgtagtgg
    21181 cggagatcga gcgcgatatg gcgctggatg tgccgatgat gcgtctggtg cagggcgatg
    21241 taggttccgg taaaacgctg gtcgccgccc tcgccgcgtt gcgtgcgatt gcccacggca
    21301 aacaggtagc attgatggca ccaaccgaat tactcgccga gcagcacgcc aataacttcc
    21361 gcaactggtt tgcaccgctc ggtatcgaag tgggctggct cgccggtaag cagaaaggta
    21421 aagcacggct ggcacagcag gaagccatcg ccagcggtca ggtgcagatg attgtcggta
    21481 cacacgccat cttccaggaa caggtgcagt ttaacggcct ggcgctggtg attatcgacg
    21541 aacagcatcg ttttggcgtg catcagcgtc tggcattgtg ggagaaaggc cagcagcagg
    21601 gcttccatcc gcatcagttg atcatgaccg ccacgccgat cccccgcacg ctggcaatga
    21661 ctgcgtatgc cgatctcgat acctcggtga tagatgagct gccgccaggc cgcacgccag
    21721 tgactacggt cgctattcct gatacccgcc gtaccgacat cattgaccgc gtgcaccacg
    21781 cctgcataac tgaaggtcgt caggcatact gggtttgtac gttgattgaa gagtcggaat
    21841 tgctggaagc gcaggcggcg gaagctacct gggaagagtt gaaactggcg ctaccagagt
    21901 tgaacgttgg cctggtacac gggcggatga aacctgccga gaaacaggcg gtgatggcgt
    21961 cgtttaaaca aggtgagcta cacctgctgg ttgccacaac cgttattgaa gtcggcgttg
    22021 atgtgcctaa cgccagtctg atgattatcg aaaacccgga gcgtctgggt ctggcgcagt
    22081 tacaccagct tcgcgggcgt gtaggtcgtg gtgcggtggc ttctcactgc gtgctgctct
    22141 acaaaacgcc gctctccaaa accgcgcaaa ttcggctgca agtgctgcgc gacagcaacg
    22201 acggttttgt gattgcacag aaagacttag agattcgcgg accgggtgaa ctgttaggta
    22261 cgcgtcagac gggtaatgct gagtttaaag tggcggattt actgcgcgat caggcgatga
    22321 tcccggaagt tcagcgcctg gcgcgacata ttcacgaacg ttacccacaa caagcaaaag
    22381 ccctgataga acgctggatg ccggagacgg aacgttactc caacgcatga ccagactcat
    22441 cacaagacaa gtcacgcagt aaatcttcaa tctggatagt gaggatatcc attgcctcat
    22501 acgcatcctg tgcaattcgc gccgccattg cgccttccgg ttcaccacgc agaatttcag
    22561 ccgctgcctg tacgccagcc acaatcattt caggtgttaa catttggcga agaaaagcgc
    22621 gattatcaga ggaccagcca tctttatggc ctaatggatg cccccagcct atccattctt
    22681 gcgaccataa cggattggct tctatgggat aaattagatg cgcggcatac tgaatagcac
    22741 acaccgcttt tcctggcgta tccagcgccc accagcgcga ccagagagaa cgaattaacg
    22801 gcgcggcccc acccagagta ttgaacaccg ccagccagca aagaacgtta ttgaaaccac
    22861 gctcgttatc catacgctgc aacgcggtgt ccagtaaaaa ttgccgtacc tgctggcgtt
    22921 gggaagcatt catcatctcc tgccagagat agggccgaca caatgccttc attaaggatt
    22981 cttcgaagtg atcaccataa ccgccagcta aaacggtttc atgccattgc gccaggcaga
    23041 gcggaaacag atagcgaaac aactctattt gcatcggttc gttataaacg agattgagta
    23101 catagtaagc actcaaatcc cattcgctga tctgctgcca ctctttagcc gccagcgcct
    23161 tgagttcggc atctgcgtaa tcaaattccg tttcccatac acagagcact ggcggctcat
    23221 caccaaaaat atggcgacag tgttcacgta gcgtttccgg ttccttccga cgctttttca
    23281 tttttaaccg gcaaaaatcg gcaacattaa atacagctta atcaccagcg cattgacgat
    23341 atcgataaag aacgcaccga ccatcggcac caccaaaaac gccatgtgcg acgggccaaa
    23401 gcgttcagtg atcgcctgca tgttggcgat tgccgttggc gttgcaccga ggccaaaacc
    23461 acagtgaccc gcagccagca ctgccgcatc gtagtttttg cccatcatgc gccaggtaac
    23521 gaagatggca tacaacgcca tgaagatggt ctgtaccacc agaatcgcca gcatcggcag
    23581 cgccagcgaa gccagctccc acagtttcag ccccatcagc gccatcgcca ggaacaacga
    23641 caggcttacg ttacccagca cggataccgc acgctcaaag acgcggtaaa agcccatcat
    23701 tgacagaccg ttgctcagaa tcacgccaac aaacagtaca cagacgaagg tcggcagttc
    23761 aaaagcagtg ccagccaaaa gttgcgcaac aattttcccc accgtcaggc agatagcaat
    23821 cagcgcgata gtttcaatca gcaccagcga ggtgatcatg cgtcccacat ccggcttttc
    23881 aaacgccgtc gggacttcct ggtcatccgg aataccgttc ggcgtggtgg agtgtttcac
    23941 cagatagcgc gccaccggac cgccaatcaa gccgcccagc accagaccga acgttgcaca
    24001 ggccatcgcc acttccgtcg cattggtgaa gccataacgt tcaatgaaca atttactcca
    24061 cgcagcgccc gtaccgtgac cgccggaaag cgtaatagaa ccggccaaca gccccatcag
    24121 cggatcaagc cctaacaggc tagccatacc aatgccaatg gcattttgca tcaccaacag
    24181 accaacaacc acaatcaaga agatgccaac cacacgccca ccggcacgca aactggcaat
    24241 gttggcgttc aggccaatgg tggcgaagaa agccagcatt aacggatcgc gcagggacat
    24301 atcaaagttg acttcccagc ccatgctttt tttcagtact agtagcgcca gcgccaccaa
    24361 caaaccaccc gcaacaggtt ccggtatggt gtatttcttc aaaaaggaga cggaatggac
    24421 caacttacgc ccgagcagca acgtcagcgt tgcggcaaca agcgttgcta aagtatcgag
    24481 atgaaacata gttactcctt tgtatccgca tcactctttc atactcgtca tacttcatcg
    24541 cgcagtggca acttcatgaa ctatgacggg tatcgagtta tgctttcctg ggtcaaattc
    24601 tcagctgtta atgaacaaat ggcatggatt ttaagcagaa aaataccaaa agttatataa
    24661 aaaagtgcat ttatctcatt taaatattta gatgaggatg tggcaaacgt ttgcttttct
    24721 ctttcggtca gctaaaatgc ccgctttgct accacgggat tgttttcgat gtctgtttcc
    24781 accctcgagt cagaaaatgc gcaaccggtt gcgcagactc aaaacagcga actgatttac
    24841 cgtcttgaag atcgtccgcc gcttcctcaa accctgtttg ccgcctgtca gcatctgctg
    24901 gcgatgttcg ttgcggtgat cacgccagcg ctattaatct gccaggcgct gggtttaccg
    24961 gcacaagaca cgcaacacat tattagtatg tcgctgtttg cctccggtgt ggcatcgatt
    25021 attcaaatta aggcctgggg tccggttggc tccgggctgt tgtctattca gggcaccagc
    25081 ttcaactttg ttgccccgct gattatgggc ggtaccgcgc tgaaaaccgg tggtgctgat
    25141 gttcctacca tgatggcggc tttgttcggc acgttgatgc tggcaagttg caccgagatg
    25201 gtgatctccc gcgttctgca tctggcgcgc cgcattatta cgccgctggt ttctggcgtt
    25261 gtggtgatga ttatcggcct gtcgctaatt caggttgggt taacgtccat tggcggcggt
    25321 tacgcagcca tgagcgataa caccttcggc gcaccgaaaa atctgctgct ggcaggcgtg
    25381 gtcttagcct taattatcct gcttaaccgt caacgtaacc cttacttacg cgtggcctca
    25441 ctggtgattg cgatggcggc cggatatgcg ctggcgtggt ttatgggcat gttgccagaa
    25501 agcaacgaac cgatgacgca agaactgatt atggtgccaa cgccgctcta ttacggtctt
    25561 ggcattgaat ggagtctgct gctgccgctg atgctggtct ttatgatcac ttcgctggaa
    25621 accattggcg atatcacggc gacctctgac gtttccgaac agccagtgtc cggtccgctg
    25681 tacatgaaac gcctgaaagg cggcgtgctg gcaaacggcc tgaactcgtt tgtttcggcg
    25741 gtgtttaaca ccttcccgaa ctcctgcttc gggcaaaaca acggagtgat ccagttgact
    25801 ggtgttgcca gccgctatgt cggttttgtc gtcgcgctga tgttgatcgt gctgggtctg
    25861 ttcccggcag tgagcggttt tgtacaacac attccagaac cggttctggg cggcgcaacg
    25921 cttgtaatgt ttggcactat cgccgcctcc ggtgtgcgta tcgtttctcg tgaaccgctg
    25981 aaccgtcggg cgattctgat tatcgcgctg tcgctggcgg ttggtctggg cgtgtctcag
    26041 cagccgctga ttttgcagtt tgcccctgaa tggctgaaaa acctgctctc ctccgggatc
    26101 gccgcgggcg gtattactgc catcgtgctg aatctgattt tcccaccaga aaaacagtaa
    26161 ttcactggcg gcggtaagat aattaccgcc gcagtctcac ctctttcacc attcccgcct
    26221 tgaggattgc gcgtaaatcg tgcataactc ccttatgtgc atttcacggg atggaagacc
    26281 atgaaattta ttgggaagct gcttctctac attctcatcg ctctgttagt ggtgatcgct
    26341 ggcctctatt ttcttctgca aacccgctgg ggagcagaac atatcagcgc atgggtttcc
    26401 gagaatagcg actatcatct ggccttcggg gcgatggatc accgtttttc cgcgccatct
    26461 catatcgtgc tggagaacgt cacgtttggc cgtgatggcc agcccgcgac cctggtggca
    26521 aaaagtgtcg acattgcgct aagcagtcgg caactgaccg aaccacgcca tgtcgatacc
    26581 atcctgctgg aaaacgggac gctgaatctc accgaccaga ccgcgccgct accgttcaaa
    26641 gccgatcgtc tgcaactgcg tgatatggcg tttaatagcc cgaatagcga atggaaactg
    26701 agcgcgcagc ggataaatgg cggcgtagtt ccgtggtcac caaaagccgg taaagtgctg
    26761 ggtacgaagg cgcagattca gtttagtgcc ggatcgcttt cgctcaatga tgttcctgcc
    26821 accaatgtac tgattgaagg cagtattgat aacgatcgcg ttacgctgac taacctgggt
    26881 gccgacatcg cccgcgggac attaaccgga aacgcgcagc gtaacgccga cggcagctgg
    26941 caagtggaaa acctgcgcat ggcggatata cgtctacaaa gcgaaaaatc gctaaccgac
    27001 ttctttgcgc cattacgctc tgtcccgtcg ttgcagattg gtcgcctgga agtgatcgat
    27061 gctcgtttgc aaggtccgga ctgggcggtg accgacctcg atctcagctt gcgcaacatg
    27121 accttcagta aagatgactg gcagacacag gaaggcaaac tgtcgatgaa cgctagcgag
    27181 ttcatttatg gttcgctgca tttatttgac ccgattataa acgcggaatt ttccccgcag
    27241 ggcgtagcgc tgcgccagtt caccagccgc tgggaagggg gtatggtcag aacgtcaggg
    27301 aactggctgc gtgacgggaa aacgttgatc cttgatgatg tggcaattgc cgggctggaa
    27361 tataccttgc cgaaaaactg gcaacagttg tggatggaaa cgacacccgg ttggttaaac
    27421 agcctgcaac tgaagagatt tagcgccagc cgcaatctga tcattgatat cgaccctgac
    27481 ttcccgtggc agctcaccgc gctcgatggt tacggtgcca acctgacgct ggttaccgat
    27541 cataaatggg gcgtctggag tggctcggcg aatctgaatg ccgccgccgc gacattcaat
    27601 cgtgttgatg ttcgtcgccc gtcgctggcg ctgaccgcca acagcagcac ggtgaatatc
    27661 agcgaactga gtgcatttac tgaaaaaggc attctggaag ccactgccag tgtttcacaa
    27721 acgccacaac gtcagaccca tatcagcctg aatggacgcg gtgtgccggt gaatattttg
    27781 caacaatggg gatggcctga attaccgttg actggcgacg gcaatattca gcttaccgcc
    27841 agtggagata ttcaggccaa tgccccgctg aaacctacgg ttagcgggca attgcatgcc
    27901 gtgaacgccg caaagcagca agtgactcaa accatgaatg ctggcatcgt ttccagcggt
    27961 gaagttacgt cggcagagct ggtgcagtaa acatcaatct cgccgggtac gactcagacg
    28021 tacccggcat tccatcaata gattacaacg taattgtcag cgcattccct tgaggcttca
    28081 ccaccagccc ctgctcactt tcagcctgcg aaccgtcttg cagaccattt actttcacaa
    28141 cattgcgcag gcacagtgtc cagttcttcg cctcgcccgc accagtcaca gtaatcgtgt
    28201 tgccagtacg tgctgctttt aaagtaaaga tcaccgatcc gtcagcagcg ggcacttcac
    28261 agacggcttc atgcccgtct tgcagattaa agaggtggaa tgccgtgcct tcgtgccaca
    28321 cgtaatcggg acgttgatcg ttgttgccca gcgccagtag agtgttatca cgcacataaa
    28381 cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg agttcatcgt
    28441 tgtgccacag gtgtgtccag cgaccttccg gcaggtagaa ctgcacatcg cccgcttcag
    28501 tgaacaccgg cgcaaccatc acgttgttgc ctaacatgta ttgacggtca aggtaatcac
    28561 aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta ccccgcgcgt
    28621 tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt tgcgtgaaga
    28681 agcgcactac atcacaggac tcatcatcgt aggcccacgg cacacgataa gatttgctac
    28741 cgtgtaaacg gctatggctg gagagcaaac caaacgcgca ccagcgtttg taaacgtgcg
    28801 ccggagcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa cctgaaaggc
    28861 caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg taacaatcgc
    28921 caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca aacaagacag
    28981 cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg tagatgtatg
    29041 cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca tcagttggga
    29101 tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga cctttcagtt
    29161 tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc agacctggct
    29221 gccatttatc ccactgccat agcgaaccgt ccgggcgttt gagtaaatag cctttctctt
    29281 gtaactcttt aaagacgggg gatttttgac cgatataggg gttaatccag acgcagattt
    29341 tcagcccttt cgctttcagg cggcggatca tcccttccgg gtcagggaaa gtcagcgggt
    29401 cccactcaaa atcgcaccac tggaaggctt tcatccagaa acagtcaaag tggaaaacat
    29461 gcagcggcag attgcgttcc gccataccat cgataaagct gtttaccgtc gcttcgtcgt
    29521 agttggtggt aaatgaagtg gttagccaca ggccgaagga ccacgcgggc ggcagcgccg
    29581 gacgaccagt aaagcgggta taacgatcaa gtaccgcttt cggcgtcggg ccgtcgataa
    29641 caaagtattc gagatattca ctctcaacgc tgaactgcac tttggagact ttctccgatc
    29701 ccacttcaaa agagacacac tggggatgat tgaccagtac cccataacca cggttagtca
    29761 tgtagaacgg gatattttta tacgcctgtt cagtacttgt gccgccgtcc cggttccagg
    29821 tctctaccgt ctggccattg cgcaccaggg cagtaaagcg ctctcccaga ccgtaaactg
    29881 tttcgccaac gccaagatca agccgctcaa acatataatt gcgttgatta ttcgtgtcct
    29941 gcacgtagcc attatttttc acctgactac cggtaatacg ttcgccgttg cgcagaaaat
    30001 ccagtgacca gaactcacct ttgctgacac gcgcgcttaa gttgccactt ttaaactcag
    30061 cataacgttc tgtgttttcg attgtgacct tcacgtcctg caaaatattg agcggataat
    30121 gaggaccgtt attcagcgcc ccctgaaaat gctcaatccg cacaccgaca ataccttcct
    30181 gtggggagaa aaagcgcagc gtaaataaag gcgtatcaag ctgccaggta cgttcacgca
    30241 catcacgggg ggcagcatag accaccattt cattaccctg ctgttcaacc tcgaatacct
    30301 gaagcgggtg aatcaaattg aggccaggtt gaatcaacca gtttccatcg ctaattttca
    30361 ttgccgttcc ttagttctgc acttcttgag aggtgaattg ttgctggcaa cggcgtttac
    30421 cctgagccag ctgttccata acggttttca gattgtgcgt cgtgagtgag tagtagcgtt
    30481 tagcgataat cgcgctcagc aaataacaga tcgccggaac aatcgtgaat agcgcaataa
    30541 tgatgctaat cgtggcgctg ttctgcgctt tttctgccgc atcatatccg ccataagcca
    30601 gcatccagcc gataagagcg ccgccgaagg ccaaccccag tttgagcaca aacagcgtgc
    30661 cagcaaaact gatcccggtc aggcgtttac cattgcacca ctcgccgtag tcgacggtat
    30721 cggacatcat tacccactgg ataggtgtca ccagttgatg caacacacca atcacgaaga
    30781 tgaagacaaa catagtgatg ctggcctgca tgggaacaaa gaacatcgcg aggctaatca
    30841 ctgccagcag ggcgttcgtc caccagaaga tagtgacttt acatttccag tcggtcagag
    30901 gttttgccag tgcggaacca atcaggttac ccacgcaata agtggtgaga aaagcgacaa
    30961 acacttccgg cgtgcccaaa atccatgtga cgtaatacat catcgcccca ccgcgtacgc
    31021 acaccgccag gatattgaaa atggttagta aaccgacaat ccgccactgg tcgttttgcc
    31081 agatatcacg taaatcttcc cgcatagacg ttgttgtagg tggtgcttca acgcgttctt
    31141 tagtggtgaa gaaacagaat gccagcatca tgaatgccac cacggaaagg accgcgatac
    31201 cgccctggaa accgagtggt ttattatcac cgccaattaa attaaccagt ggcatcatca
    31261 gaacagtaga aagcatgcct cccgcggtcg ccagcacaaa acgccaggat tgcagcgaga
    31321 tacgctgagt cgggtcattg gtgattacac cacccaatgc gcagtaaggg atattgacga
    31381 cggtataaag taaggtaagt agggtgtaag taattgctgc atagatcatt ttgccgttca
    31441 tactgagatc tggcgtgcta taggccagta cacagacgat cccgaatggc agtgcgccaa
    31501 acagtaccca cggacgaaat ttaccccagc gagagcgcgt tcgatcggcc aacaacccca
    31561 tgcaaggatc ggaaatcgca tccagtgcac gagcgaccaa aaacatggtt ccgacaaaac
    31621 cggcaggaat gccaaaaata tcggtataaa agaacatcat atataacatt acgttatcga
    31681 aaataatgtg gctggcggcg tctcccatgc cataaccaat tttctcttta acggacaaca
    31741 cttcactctt catctgcttt tccttgatgc tataggggtg ctgagaccga taacgcattt
    31801 tgtaaaccat ccctgtgaga gcgaatattc cgatatctgg ttattaattt atgattcttg
    31861 ttttatgtga tcgtggtagc gttaatttcg ctcatatatc attgtaaaat ataggtttta
    31921 tatgaactat aatgctttcg tgataatacg ctgcgtgtat taggcggaaa aaactgatct
    31981 gggggatgta gaaactcaag gaagtagcta taatgcgccc cgcctccatg tagcaatcga
    32041 ggcgcggaag atcgtcgtct ccggtgaggc ggctggactt caaatccagt tggggccgcc
    32101 agcggtcccg ggcaggttcg actcctgtga tcttccgcca aaatgcctct tctgacgttc
    32161 tccaacccct tgtaatactg tcaatccaga tattttctac tgctcaatat tcaatcggct
    32221 tcaggaatac ctggtctaag gttttgtgat ggacgatgag cggctgaaga atccgcctgt
    32281 cggttcttct gtcctcgaac gcatccgcga tatgcgcgcc agcatgtgcc gtgtttattt
    32341 gcaggtgatt tgccccctgc tggctctcag ccaactgtat cgccattaag gtgaagatgt
    32401 tgttttactc gccccagaac accctttttt atacgactat tctatttctt taaccgctgg
    32461 cgaaaaaaac tgactataaa cttcaccaat aatcagactg ccatcaagat taatttgttc
    32521 atttaatata tcaaatcaac ttgttttatt tttactatca aaaatagctc attgccttac
    32581 tataattaat atataaattc ggtaattaat tcttaacatg cttttgctaa taatctcaat
    32641 tgctggccct ataatattgc gctagcattg ctttctggtt gtatcagcga tactcaaaaa
    32701 ttcttaatac aatactcatt cgactggtac ttatttgtaa ctcagttata ttttgtcgcc
    32761 cggtgattca gaagaatgca aaaaacggct accactccat caaaaatact tgatctcact
    32821 gccgcggcat ttttacttgt cgcctttctg acgggtattg cgggcgctct tcagactcct
    32881 accctaagta tattcctcgg agatgaactg aaagcccgtc ctataatggt agattttttc
    32941 ttcaccggta gcgctattat gggaattctg gtcagtcaat ttctggcaag gcactccgat
    33001 aaacaaggcg accgtaaatt actgattctg ctatgttgct tatttggagt gctggcctgc
    33061 acgctttttg cgtggaatcg caactacttc attctcctct caacgggcgt acttctgagt
    33121 agttttgctt ccaccgcaaa cccgcaaatg ttcgccctcg cccgtgaaca cgccgacaga
    33181 acaggccgtg agacggtcat gttcagtaca tttttacgtg ctcagatctc gcttgcctgg
    33241 gttatcgggc caccgctcgc ttatgaactg gcaatgggat ttagttttaa agtgatgtat
    33301 ctcaccgctg ccatcgcatt tgttgtttgc ggactgatag tctggttgtt tttgccatca
    33361 atacaaagaa atattcctgt cgttacccaa cccgtagaaa ttttaccctc cacccacagg
    33421 aagcgggata cgcggctact ttttgtggtc tgttcaatga tgtgggcggc gaataatctc
    33481 tacatgataa atatgccgct atttattatt gatgaactgc atctaaccga taaactggct
    33541 ggagaaatga tttgtatcgc tgccggtctg gaaattccga tgatgttaat cgcaggctat
    33601 tacatgaaac gtattggcaa gcgactatta atgctcattg ctatcgtgag tggaatgtgt
    33661 ttttacgcca gcgtactcat ggcgacgact ccggcggttg agctggaatt gcaaattctt
    33721 aatgccatct tccttggtat tctctgtggt atcggcatgc tttattttca ggacttgatg
    33781 cctgaaaaaa taggctctgc gacaacgtta tatgcaaata cttcacgcgt cggctggatt
    33841 atcgccggct ctgttgacgg aattatggtt gaaatctgga gctaccatgc gttgttctgg
    33901 ctggcgatag ggatgttggg tattgcgatg atttgcctgc tgtttattaa agatatttag
    33961 ctcaccagtc tgggctaaga aaaatcggcg tggcgcaaac agagccatcc tcgccatttt
    34021 tgctatcatg cctgcataca taaacgacaa aacagtatgc agagggaaaa atgggttcca
    34081 ccagaaaggg gatgctgaac gttctgattg ccgccgtgtt gtggggaagt tcaggggtct
    34141 gtgcgcaata catcatggag caaagccaga tgtcgtcgca gtttttgact atgacgcgtt
    34201 tgatattcgc cggtttgatt ctactgacgc tgtcatttgt tcatggcgat aaaatctttt
    34261 ctattattaa caatcataaa gatgccatta gcctgctgat tttttccgtg gttggcgcgc
    34321 taactgtaca gctcactttt ttgctaacca tcgaaaaatc gaacgcagcc acggcaacgg
    34381 tgctgcaatt cctctcaccg acgattatcg tcgcctggtt ctcactggtg cgtaaatcgc
    34441 gcccgggcat tctggttttc tgcgctattt tgacatcgct ggtcgggact tttttattgg
    34501 tgacacacgg taatccgacg tcattatcga tctctcctgc cgcgttgttc tggggcattg
    34561 cctcggcatt tgctgctgca ttctatacca cctatccctc aacactaatt gcccgctatg
    34621 gcacgttacc agtcgtcggc tggagtatgc tgattggcgg tctgattctg ttgccttttt
    34681 atgccagaca aggaacaaac tttgtcgtta acggcagttt gattctggcg tttttttatt
    34741 tgatcgtcat tggtacgtcc ctgacattta gtctgtacct gaaaggagca caattaattg
    34801 gcggtccaaa agccagcatt ttgagctgtg cagaaccatt aagtagcgcg ctactctctt
    34861 tgctgttgct ggggatcacg ttcacattac cggactggct gggaacgctg ctaattctgt
    34921 catcggtaat tttgatttca atggattccc gtcgccgcgc cagaaaaata aatcgtccgg
    34981 cgcggcatga gtgagaatta ccagccaggc accgcgccac cgttaaaaat ggtttctgcc
    35041 gctttagcga cttcgggtga ttgataagat tgcagaaatt ccttcacgtt ttctgcattc
    35101 ttattatctt cccgtgccac caaaatattc acatacggcg aattcttatc ttcaataaat
    35161 acgctgtcgt gcaccggaga aagccctgtc tgctgaatgt aagtggtgct gataatcgct
    35221 acatcaactt tgggatcatc cagtacgcgc gggagctgcg ccccctcgag ttccataatc
    35281 tgcaaatgac gaggattatc ggttatatcc agcgcggtag gtaataaccc ttttccctct
    35341 ttcagagtaa ttagcttctc tttttgcaac agtaaaagtg cgcgaccaag gttggtcggg
    35401 tcattgggga tcgccactgt cgcgccttct tttatttgcg ccacggtttt gatttttttg
    35461 gaataacccg ccatagggaa gacaaacgta tttcccaccg ctaccagttt ataaccatgc
    35521 gcctgattat cttgttcaag aaagggtcga tgctggaaga cattcgcatc aagttcgcca
    35581 tgatttgtcg catcgttcgg tagcaacgaa ccgctaaatc ctaccagttc aacatcgagg
    35641 ccatatttct cttttgccac ctttttagcg acttccgcga catcttgttc tgcgccattt
    35701 ataacgccaa ctttaatgtg ttttgcatcg ctgctactct ggtcgcaacc tgccagcaga
    35761 attccggcca gcaataatgc ggcccctgtc cgtagatgat gtgttgtcag tttcattttt
    35821 ttatcctttt attaatgatt acctgactat agcggtaagg tcgctgcggt ttaaaaaacg
    35881 aaacgctatc gataagaata aaaaggaata aaagtggaat ataaagtctg gcacttctta
    35941 ctgaccacgc aagcgcgttt cgtacagcat gatgagagcg atgaaagcaa actacactta
    36001 tgcttcattc gatacacatt tgtaaaaggt tgaatccatg aaaccaacga cactactgct
    36061 tattttcact tttttcgcta tgccaggcat cgtatacgcc gagtcgcctt ttagctcact
    36121 gcagtccgca aaagagaaaa ccacggtatt acaagattta cgtaaaattt gcacgccaca
    36181 ggcgtcatta tcagatgaag cgtgggaaaa gttaatgttg tcagatgaaa acaataaaca
    36241 acatatacac gaggccatcg tggcgatgga acgtaataat cagagcaatt attgggaagc
    36301 gttgggtaag gtagaatgcc cggatatgta agaacagcaa caactactga ccaacgcttc
    36361 tttacctgat atcaggattt cttcattttc acctttgcag taaccaacaa tgcagtcagc
    36421 aacatcaatg tgccggacaa catcaacggc gaagtcagac caatattatc cagcgcataa
    36481 ccgccgattg ccgcgccaca ggtattagca agctgaataa ccgccacctg aatagacccg
    36541 gctttttctg cctgatcggc cagcgagcgg gtgatccacg ttgaccagcc gacgggaacc
    36601 aatgcaaaag ttagccccca gataatcgcc acgccggtag caacgatttt atcgcttccc
    36661 cacaacgtca gcaccaacgc actcacagcc agtattaacg gcgcgcctgc taaggccagt
    36721 tttaccgaac gtttaagaat gaatgacgaa agcgacgtac cgataaagct ggcaatgcca
    36781 aaactcaaca gcaccagcgt taagccatcc acgctgaatc ccgccaggtt catatacacc
    36841 gggcgaatat atgtgaagaa agcaaactgc ccggcgaaag acatgaagat ggcgatcatc
    36901 cctgccatca cacccggacg ttgtaataag cggaaagtat tttgtttctg atgcgagggt
    36961 tcgcctggca gtgaaggcaa tgatttgata atccagaaaa tacacagcac gcccatcacc
    37021 gccgccgcat taaagacatt gcgccaaccg ataagctcgc ctaaaaaact gcccaacggc
    37081 gcggcaatca ccagcgcaat agaaaccgcg ccgaagatca ccgacagcgc cttcggcacc
    37141 gtacgcggcg gcaccagacg catggtcagc gacgccgaca tcgcccagaa cccgcccagc
    37201 gccagcccca gacaggcacg accgattaaa agcaaactga atgagttagc aaaggaaacc
    37261 agcaagcagg agagcgtcag caaaacggca aacaaaataa caacgtagcg gcggtcagta
    37321 gcctgaattg tctgggtaat aaacaaactg gcaaacattg ccacaaaggc ggtcacggtc
    37381 accgattgcc cggcaacccc ttccgaaatg cctaaatcct gggccattgg cgtcaacaaa
    37441 ctgacgggca aaaactcaac gataatcaga caggcgacac aaaacgccac cgagaaaacg
    37501 gctgaccagt tcggtcgggt gatggcatcc gcgccgcggt tttcggcaat aaattcactc
    37561 atggtgttac ccgtggcatg tttcagggga aaagccgacc actttaacat tgaaatgtgt
    37621 gacgcattta acgtttttgc aacttttacg gtgttgcttg agctggagtt aagatcgaaa
    37681 cggaacaggg caaagattac cggatgcggc gtgaacgtct tatccacccg acaggtcagg
    37741 cgcggtctgt aggctgataa gacgaaacgg tttcaatgca accagtgaac gccctcggca
    37801 ggctgaaaga acgcgttata gcgcatctga aaagcattaa tatcctgcat ttcaggttcg
    37861 gtttcgcgca gaaaacctag cgccagcctg acctgagcgt cggtaatcgg cgcggcgagg
    37921 cgagcttttt gtaaaatccg atgccattgc agcaggttct gttcgctacc atcgacaatg
    37981 acaatttgcc agatgagcaa cacctgcgcg gcgttttgca ggtgcgactc atccggacga
    38041 tgcaaataac gtaaaaattc gtcaccagct gactttccca tttgatcgac catcgactcc
    38101 acgggtacga catcgatgtt cagccgatcg ctgagacggc gaatcgcttt tctggccccg
    38161 gatgtcagca cccgaaaacc aaccacaaac actaccgcca gcgtggcgag cattatccag
    38221 atcatgagtt ctcctgcgta atcagaactc atgatatctg gaaaccctcg ccagttacta
    38281 atccaccaga ataatcttca gtgcaaacag agctgcgacc accaccacac acaggttcag
    38341 atcgcgccag cgcccggtgc atactttcat gatgcagtac gacataaagc cgagtgcaat
    38401 cccttcggtg atcgagaaag taaagggcat catcaccgtg gtaataaacg caggcaccga
    38461 ttcggtaaaa tcatcccagt taacgcgcgc caggctcgaa gtcatcagca cgccaacaaa
    38521 gattaacgct ccggcggttg cgtaaggagg aaccatcgcc accagcggtg agaagaacat
    38581 aactaacagg aacataacgc caaccacaac cgcagtcagc cccgtgcgac caccgactgc
    38641 cacaccagaa gtactttcaa tataggcagt aacagacgag gtgccgataa acgcccccgc
    38701 caccgaactg acgctatcaa catacagcgc cttattcata ttggggaatt taccgttact
    38761 atctattaag cccgctttat cagttacacc aattaatgtt cctgatgaat caaataggtt
    38821 gatcagcata aaggagaaaa tgataccggc gagttcaagt gttaacgcgc cgctcaaatc
    38881 tacttcacca atgacgccgc taatatcagg cggaatggaa tagacgccgc taaaatgaac
    38941 atcaccgaaa aataatccac agcaagacgt caccacaata gaaaccagca ccgcggcatg
    39001 aaaatgacgt gatgacaaca cggttataat aaaaaaccct aaaataccta acaacacgcc
    39061 gtgagaactt aaatcgccaa tcatcaccag cgtgtcttta ttggcgacaa taacgccagt
    39121 attttttaat cccattaagg cgataaataa tccaattccg ctggtgatac caatacgtaa
    39181 acttaaggga atgttggaga tcatccagta ccggatacga aacagcgtga gcaaaaatag
    39241 tccaactgcg ccccagaata ttgcgcccat cccggtctgc caggagatgc ccatcgcccc
    39301 cacgaccacg aaggcaaaga aggcgttcag ccccattgcc ggagccagcg ccacgggtaa
    39361 gttagcaaat atccccatcg caatactgcc gataccggca atcaaacagg tggtaacaaa
    39421 caccactttc gggtccattt gtgccgcgcc gaggatttgc gggttcacaa aaacgatgta
    39481 caccatggtt aaaaaagtgg tcatccccgc aatcaattct gtgcggacgg tggtcccatg
    39541 ctgaggtagt ttaaataatc gcgaaagcgt ccctgattca ttactcacgt aatcggtatt
    39601 gtcattattc atttttttgt ccattggagg agatttaatc ccttgctttt gtaaaacaaa
    39661 tgacaacaac ggttcagtga taattatcac atttcaattg cacattaatg gatattcttt
    39721 aataatctcg cgacgtttct ttatgataaa taataatcaa attgataaaa tcaaaatgag
    39781 aaaaatatga ataattctat taaccataaa tttcatcaca ttagccgggc tgaataccag
    39841 gaattgttag ccgtttcccg tggcgacgct gttgccgatt atattattga taatgtctct
    39901 attctcgacc tgatcaatgg cggagaaatt tccggcccaa ttgtgattaa aggacgttac
    39961 attgccggtg ttggcgcaga atacgctgat gctccggctt tgcagcggat tgatgctcgc
    40021 ggcgcaacgg cggtgccagg gtttattgat gctcacctgc atattgaatc cagcatgatg
    40081 acgccggtca cttttgaaac cgctaccctg ccgcgcggcc tgacgaccgt tatttgcgac
    40141 cctcatgaaa tcgtcaacgt gatgggagaa gccggattcg cctggtttgc ccgctgtgcc
    40201 gaacaggcaa ggcaaaacca gtacttacag gtcagctctt gcgtacccgc cctggaaggc
    40261 tgcgatgtta acggtgccag ttttaccctt gaacagatgc tcgcctggcg ggaccatccg
    40321 caggttaccg gccttgcaga aatgatggac taccctggcg taattagcgg gcagaatgcg
    40381 ctgctcgata aactggatgc atttcgccac ctgacgctgg acggtcactg cccgggtttg
    40441 ggtggtaaag aacttaacgc ctatattact gcgggtattg aaaactgcca cgaaagttat
    40501 cagctggaag aaggacgccg gaaattacaa ctcggcatgt cgttgatgat ccgcgaaggg
    40561 tccgctgccc gcaatctcaa cgcgctggca ccgttgatca acgaatttaa cagcccgcaa
    40621 tgcatgctct gtaccgatga ccgtaacccg tgggagatcg cccatgaagg acacatcgat
    40681 gccttaattc gccgcctgat cgaacaacac aatgtgccgc tgcatgtggc atatcgcgtc
    40741 gccagctggt cgacggcgcg ccactttggt ctgaatcacc tcggcttact ggcacccggc
    40801 aagcaggccg atatcgtcct gttgagcgat gcgcgtaagg tcacggtgca gcaggtactg
    40861 gtgaaaggcg agccgattga tgcgcaaacc ttacaggcgg aagagtcggc gagactggca
    40921 caatccgctc cgccatatgg caacaccatt gcccgccagc cagtttccgc cagcgacttt
    40981 gccctgcaat ttacgcccgg aaaacgctat cgggtcattg acgtcatcca taacgaattg
    41041 attacgcact cccactccag cgtctacagc gaaaatggtt ttgatcgcga tgatgtgagc
    41101 tttattgccg tacttgagcg ttacgggcaa cggctggctc cggcttgtgg tttgcttggc
    41161 ggctttggac tgaatgaagg tgcgctggct gcgacggtca gccatgacag ccataatatt
    41221 gtggtgatcg gtcgcagtgc cgaagagatg gcgctggcgg tcaatcaggt gattcaggat
    41281 ggcggcgggc tgtgcgtggt acgtaacggc caggtccaaa gtcatctgcc gttgcccatt
    41341 gccggcctga tgagcaccga cacggcgcag tcgctggcgg aacaaattga cgccttgaaa
    41401 gccgccgccc gtgaatgcgg tccgttaccc gatgagccgt ttattcagat ggcgtttctt
    41461 tctctgccag tgatccccgc gctaaaacta accagtcagg ggctatttga tggcgagaag
    41521 tttgccttca ctacgctgga agtcacggaa taataaaaaa gcccggcgtc atgccgggca
    41581 aaagtcacca gttacgttta tgccactgtc aactgctgaa tttttttctc gcggcggatt
    41641 ttgcgttctt ccatcaccgc cactatcgcc atcaggcaga tacaaccaat cgcggcgata
    41701 tcaagtgcgg cgaaggtgcc tgcccagccg gtaaggccga ataccggcgt cccatcggca
    41761 atcattccca gacctaactt ggcaaagctg tcaccaatca ggtaagcaaa ggtgccttta
    41821 ataccatcgg cagcgccaat cgctttttta ggtacaaagc caacagcagc cacaccaatc
    41881 aacaattgcg ggccaaagac caggaaaccc aacgcaaaga gagaagccag atagatatat
    41941 tcgttgctgg catgttgata cacaccgagc gtggcgataa tcagcgccag cgcgatgcag
    42001 gccaccaggc cacggcgacc gttcgccagg tcagagagcc agccccacag cagcgtaccg
    42061 accagtgcac cagcttcaaa cagcgtaaag ccctgaatcg ccaccgcttt agagagtttc
    42121 agttcctgga acgcgtatac ggttgaccac tggtcgatac caatacgtac cacatagagg
    42181 aaaatgttgg cgaagcacag cagccagatc actttgtttt tcagcacata ctcaacaaag
    42241 atctgccact tggtcatatc ggtagattct gtctctttgt cctcttcgct gatctcctcg
    42301 ccgaacagtt cttcagcttt gccgaggcca taagattccg gggagtcgct gccgtaacgc
    42361 aggccgataa aaccgacaat cagcgcgata atcgacggga agataaacat gccgatgaca
    42421 tggccatcga acaggtaatt tgccccgaac agcgccacac ctgctgcgcc tgcaccgcca
    42481 aggttgtgag aaatattcca gaaaccgagg aatgtcccgc gtttacgacg cggcgtccat
    42541 ttggtgatgg tggagtaact gcacgaaccg ccggtactct ggaaaaagcc gcttaaggcg
    42601 tagaaggcaa tcatcaggaa caggctaacc gagccgctgc ccatactggc actgaagccc
    42661 agcatacaaa tggcagacag gatcagcatg aacggcagga attgtttggt gtttttgccg
    42721 tcggcgtagt aggaaaccag cgttttaccc acgccataag tgatggagaa acccaggccg
    42781 atcatcccca gctgcgtcat gctcaacccg taggtcgaga tcatgtcgtt ctgcgcgatg
    42841 ttaaagttct tgcgaatcag gtacatcgtc aggtagccga taaagaccac caggtaggat
    42901 tgcatgaacg gtttgaacca cattttgcgc cgcacttcga gcggaaggtc cagggtcggc
    42961 ttgcgaacct ggtttaagaa agccagcatg ggttactcct gatttccctt catacttcaa
    43021 gccgcaggtg ccttagctgc acgcattcct tccggtcaca tagttcacta tgctcccggg
    43081 actcacgcgt ttgctgcgtt cctgcaactt gaattatttt gggaaagaat taatgaatga
    43141 tggcattgta aaaatcagca gcaaatcagg cctgagatag cgtccaggca aaacctggaa
    43201 aatttcttag ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc ttcgcgcggt
    43261 gcctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa ccccggcggc gatagagata
    43321 accacaaaaa atccgctcca gtgccaggta tcgagtactt tcgccagcgg ccaaccagca
    43381 agcgacgccc ccagataagc aaacaagccg acaaaccccg tcgccgcccc tgcagcctct
    43441 ttgtgggaac actctgccgc cgccatgccg attaacatct gcgggccaaa gacaaaaaaa
    43501 ccaatggtga agaagcaggt tgcctgcatc acgtagctgg caaatggcat cagccacagc
    43561 gagccgactg aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc acggttgccg
    43621 ttaaacaatt tgtccgagcc ccaaccggct accagcgcac cgataaatcc gcccagttca
    43681 aacatcgtca ctgccgtatt cgccgtgacc agatcgacgc ccagcgtctc ggacatatac
    43741 aaattgcccc agtcgttgat tgccgcccgg accacataga ccagcacata gcaaaacgaa
    43801 agcagccaga tatacggatt cagcaacaca tatttggtca ggatctcttt acgcgtcaac
    43861 cctgcccctt cttgttgttg agcaatttcc agcgcgtcgt gccgccattc accgaccgcc
    43921 ggtaaaccta acgcctgcgg gcgatcgcgt agccgccagc agagaaaaat ccccacgact
    43981 atcgccatac aaccagcaat catcatcccg gcacgccagc cgtaatgcag cgcgctcgct
    44041 gccatcacaa tgggaatgag tgcgccgccg acgttatgcg ccgtgttcca taatgcccac
    44101 caaccgccgc gctcggtacg tgaataccag gccgttaaca gacgcgcaca caccggtgaa
    44161 ccccagccct ggaaaaaggc gttcagcacc cagagcacgg caaacgccca tagcgacgtc
    44221 gagaagccaa acagaatgtt catgatgccc gtggcgataa gccctatccc cataaaataa
    44281 cgggcatttg agcgatcgct gacaatgccg gagacaaact tcgacacgcc gtaagtaatg
    44341 taaaacaggg tcgctaacag gccgatatcg ctacggctga gcacgccgtt agcaaggatt
    44401 tctggtaccg cggcgttaaa acttttccgc gtgaagtaaa acagcgcgta acccagccag
    44461 atggtcagca gaatatgccg acgccagtag cgatagcggg catcaatttc atatttatca
    44521 gtcattaatg gcgcatcggc aggcgctttc agaaacggca acatcatcaa accttagaca
    44581 tagcgttgag gtagagaaac gctgacacgc gtgccgtgca gacaggaaat gtgtaatgtg
    44641 ccacccagcg ccgttacgcg ctcgcgcatt ccggtgaggc caaaaccttg ttgcccggaa
    44701 cccggcggca aaccgctgcc atcgtcttca ataaccagca tcaaccgttc atcctgctgc
    44761 cagccttgca gggtgaccgc gctggcatca gcatgtttca caatgttgtt cagcccttcc
    44821 tggcagacac gaaacagcgt cacgcgctgg ttttcgctta acgctgattc atcgattcgc
    44881 cattcgagat ggctgacaat accgcgccct tccagctcca tttcccgcat cagtgagcgg
    44941 atggcctgct ccagggtgag atcatccaac tggcgcggac gtaaccgacc caacaaacgg
    45001 cgcaccgcgt cgtaaacgcc cagcgatagt tgttcgatga gctgcccgct ctgcttcacg
    45061 ctggcgttat ctgccgccag ccgctgaaca atgcccgcct gagtacgaat ggctgtgatg
    45121 gtctgaccga tatcatcatg cagctcacgc gccacatcac ggcgcacgct ctcttcggtt
    45181 tccagcaacc gttcagccag atgctgattg cgcgccagtt ccttttgcag cgactggtta
    45241 agttcacgca accgctggat gccagcgcca agcaacaacc ctgtcagact ttgcaccagc
    45301 agcgagagca ataaatccac cggatgatcg cgccaggttt gactggcgat cagcgcgatg
    45361 gcgttcatca acgtcgcaat cagcgcccct tgccaaccat agtgccaggc cagcgcgata
    45421 atcggcagcg ccagacaaaa tggcgtaaag cgcgacagtt cgtccggcaa tcccaactgg
    45481 agccagagac tgataacaaa cagcagcaag taccagacca gatgtcgccc gcgccagttg
    45541 atcggctgag aaaccagtga cgggccgagc ggcagccagg tgttattggc gagatagtgc
    45601 cagaacacca gacatatcgg ggccagcgtc aggccgccag ttaaagtcag caacagcgca
    45661 ttccacgact ctttgccgtg ccaaagccag ggcagcgact gcaacaacgc cgccgccgtt
    45721 aacgccgcac cctgtaacag caaggtgcgc cagtcacgct gatggcgata gcgcgagatc
    45781 agcgccacgg gcagtaacgt cagtaaacta ccgatcatca ataacggaaa atgggttaaa
    45841 ccgaccgcct gcgttagcca gtaaatcagc agccactccg cgcccagcag caccggccag
    45901 tatccgcgcg ggcattgcag cattagcccc agacgcagac caaacggaaa taacagcacc
    45961 gccatatcag ggcgctcaac caggtgcagg ctgatactcc acaggcaaaa ccatgcggca
    46021 gagaagataa aaaagcaggc aataacggta attaagcggg agaacaacgt cttcatcacc
    46081 agccatcaaa catgcggcgc gccagctcta cgtcgttact gacacccagt ttttccatca
    46141 ggttggcgcg atggacgtgc accgttttcg gcgacaagcc cagttcggca gcaatctctt
    46201 tcaccgccat tccttgcgcc agtttttccg ccacctgccg ttcgcgtttg gttagcgggt
    46261 cctgacgacc ggatgccagt ttaatggcaa tatccggcgt cagataacag ccgcccgtgg
    46321 caaccgtatg caccgccgca atcagttcgt cagggctaca gcgtttggag agaaagccgc
    46381 gtgcccccgc gttaagcgcc tgctcaacca gcgccggact gtcatgaaca gagagcatta
    46441 ttgtcgccat acctttcggc agctggctta gcagttccag accggagata tcgggcatgg
    46501 agatatcgca aatacacacc tgcacaccgc gccccggcag ccccgccagc gcctcgcgcc
    46561 ccgaaccaaa ctcggcaact acctgcaaat caggttccag ccccagcagc tgcgcaaagc
    46621 cggagcggac gatgaggtga tcgtctataa gggcaacggt gatcatggtc ttgtcctggc
    46681 gggtaaaaaa aaacgcgctt accttaacga taagcgcgat gttgttcaag ccttgagcgg
    46741 ttactgaaaa aacaccgcaa tcttgttaaa catagtcgga tcggactgat tacgctgcac
    46801 tttcaccaca tcttccagct tatcgatttg gcttatcatc tgctccagac gctggtcgtc
    46861 attgaccagt agccagatat ggcttttgtc gctgtcctga atcggcagac aaagaatgcc
    46921 ttcaacgtta aaagcgcggc gggcaaaaag gccacaaacg tgggtcatta cgcccggatg
    46981 gttgcgaacg gtgagctcca gaatcacgtt gtcatgagtt gtgttttgca tggcttattc
    47041 ccccaccatt tcagtattcg ccgcacctgg cggcaccatc gggtaaactt tttcttcggc
    47101 atcaatgcgc acatggatca gcgccgggcc agggcgattg atgatttcct gcaatgcagc
    47161 ctgcggatcg gcttcgttat tcaaatcaca ggtttcgagg ccgaatccgg cggcaatctg
    47221 cataaagttg atttttcccg gataggtggc ggcaaaaacg ccttgctcgt agaacagact
    47281 ctgttgctga tgcaccagcc ccagcgcttc gttgttcatc agaatgattt tgacatccag
    47341 ctgattttca ctggcggtcg ccatctcctg aatattcatc atcaggctgc cgtcgccgga
    47401 gaaacacaac actttgcgat ccgggttcgc cagcgccgcg ccaatcgccg caggcaggcc
    47461 aaaacccatc gtgcccagcc caccggaggt cagccactgg cgtgggcgat tgagcggata
    47521 agcttgcgcg gtccacatct gatgctgacc cacatcggtg gtgataatcg cattgtcatc
    47581 gacacaggcg gcaacggcgt tgatcaggcc gtaatggctt aatggatcgc acgctttcgg
    47641 gattggacac gggaattcgc gctgcaaatc cgctaccaac tggtgccact ctgcacgcgg
    47701 ttgcgcttcc acctgcggga tcaattgcgc cagcacgtca tcaacatccg cctgaatcgc
    47761 cacatgcggc tgcttgattt tacccagctc tgcgcgatcg atatcgacat gaataatttt
    47821 ggcattcgga cagaactgct cggttttgcc aatcgcccgg tcatcaaaac gcgcaccgag
    47881 cactatcaac aaatccgcct cctgcaaaat atagttggtg ctgcgcacgc cgtgcatccc
    47941 cagcataccc agcgacaacg gatgcgcttt tggcaacatg cccagcgcca ttaaagtcat
    48001 ggtggtaggc agttgcgctt tctccgccag ttcacgcacc cgtgcgggcg cattgatcac
    48061 accgccgccc agataaagca ccgggcgttt ggcagcgtta atcatcgccg ctgcgtcacg
    48121 aatgctttct tcgctaaagg cgggggcggc ggctttttct gccatagcgg gctgtgtttc
    48181 aatctcaaaa actgccgttt gcacatcctt aggaatgtct atccacaccg ggcctgggcg
    48241 gcctgattgc gcaatgcgga aggcatcgct catgacctgc gggagttctt cgatatgtct
    48301 gaccagatag ttgtgtttgg tgatggggat agagatgccg taggtgtcca cttcctggaa
    48361 gacgtcggtg ccgatcatcg aggcgggaac ctgaccggtg atgcaaatca gcgggatgga
    48421 gtccagccgc gcatcggcaa tggcggtcac caggttagtc gcacccggtc cgctacaggc
    48481 catacagacc gccggtttac cgtcggtgcg cgccatcccc tgagcgataa atcccgcgcc
    48541 ctgttcatga cgggccagaa tatggcggat ttgcgtgctt tggcttaagg catcgtaaac
    48601 aggcaggata gaaccgcccg gaatgcccgt cacaatctta atgccctgct gttccaggaa
    48661 atgaacgata aattctgcgc cggtaaagcg cttacgcgtc gatgttgtgc ccgaacttgc
    48721 catgctccag tccttttctt ctgggccgac tttccgggag gtgcttaaac gaaaaacccc
    48781 gcccggtttg cgccggcggg gttttggaat cgtgtgttgt tccagtccct acggcgcatt
    48841 gccgacgacc accaccacac gcacgacgac cactgcggcg gatagcgcag ttggtagtag
    48901 ttttgcgttg aacatggaag tagtcattgg ggaccttgtt ggttttgtgt ttaacaatat
    48961 ttatacaagc acagctttac aggggagaca atggaaaatt tttcagcaag ggaaaattga
    49021 ggggctgatc acgttttgta ctgaattgca ggtaacaaaa aaccccgccg gagcgaggtt
    49081 tcatcagccg cctgcggctg gtaaccgcaa agcacactgt attatgtcaa cactgaaagt
    49141 atacgtgttc cgcgcagaac gcgcaatttc ggcacgaatt ttgacgtatt tagggcatag
    49201 ttgagtatcg atcacagttt gcgttttgtc caaatattac tgtttattta tacagtaaac
    49261 ttctataata tcactgtacg caatgtgtta tgcgggggcc gcatcgttac ccggcgcact
    49321 aagtcctggc tgaaacgggt ggtgccgtca gcgccttaac cccgcgtgag cacactgtgt
    49381 tatgtcaaca agcacaacgt ttctccttaa gataccgcgt gcacaacagc tggcaacagg
    49441 cagcggaaag gtacgtcagc tggcagtgct cctgaaccac aggagacgcg tatgagcctg
    49501 gtggatatcg ccattcttat cctcaaactc attgttgcag cactgcaact gcttgatgct
    49561 gttctgaaat acctgaagta attcagattc aagtcgcacc aaaggggagc gggtaaccgc
    49621 tcccctttta tatttagcgt gcgggttgga gttgggggct tcttctacca gcagaatgtt
    49681 tgcacctgcg ctgcctgtgc ccctcaccct agccctctcc ccaaaggggc gaggggaccg
    49741 tcccgtgcaa tgtttagctc attttctgaa agggaacggt ggtttccccc tcgccccttt
    49801 ggggagaggg ttacagttcg cacgtaatcc ctcccggcaa catttattgc cgcttttgtt
    49861 tacatattct gccgctaaac aattccccat tcctggcgta tatctggcta acattcatca
    49921 atgtgataga ttcctctccc gcatttatgg gaatgcgtag tgacttattc taattatttt
    49981 tataaaagca tccgtgataa tgaaaaggca aagaaacgtc aatttgttat tgatgttggt
    50041 attactcgtg gccgtcggtc agatggcgca aaccatttat attccagcta ttgccgatat
    50101 ggcgcgcgat ctcaacgtcc gtgaaggggc ggtgcagagc gtaatgggcg cttatctgct
    50161 gacttacggt gtctcacagc tgttttatgg cccgatttcc gaccgcgtgg gccgccgacc
    50221 ggtgatcctc gtcggaatgt ccatttttat gctggcaacg ctggtcgcgg tcacgacctc
    50281 cagtttgacg gtgttgattg ccgccagcgc gatgcagggg atgggcaccg gcgttggcgg
    50341 cgtaatggcg cgtactttgc cgcgagattt atatgaacgg acacagttgc gccatgctaa
    50401 cagcctgtta aacatgggga ttctcgtcag tccgttgctc gcaccgctaa tcggcggtct
    50461 gctggatacg atgtggaact ggcgcgcctg ttatctcttt ttgttggttc tttgtgctgg
    50521 tgtgaccttc agtatggccc gctggatgcc ggaaacgcgt ccggtcgatg caccgcgcac
    50581 gcgcctgctt accagttata aaacgctttt cggtaacagc ggttttaact gttatttgct
    50641 gatgctgatt ggcggtctgg ccgggattgc cgcctttgaa gcctgctccg gcgtgctgat
    50701 gggcgcggtg ttagggctga gcagtatgac ggtcagtatt ttgtttattc tgccgattcc
    50761 ggcagcgttt tttggcgcat ggtttgccgg acgtcccaat aaacgcttct ccacgttaat
    50821 gtggcagtcg gttatctgct gcctgctggc tggcttgctg atgtggatcc ccgactggtt
    50881 tggcgtgatg aatgtctgga cgctgctcgt tcccgccgcg ctgttctttt tcggtgccgg
    50941 gatgctgttt ccgctggcga ccagcggcgc gatggagccg ttccccttcc tggcgggcac
    51001 ggctggcgcg ctggtcggcg gtctgcaaaa cattggttcc ggcgtgctgg cgtcgctctc
    51061 tgcgatgttg ccgcaaaccg gtcagggcag cctggggttg ttgatgacct taatgggatt
    51121 gttgatcgtg ctgtgctggc tgccgctggc gacgcggatg tcgcatcagg ggcagcccgt
    51181 ttaagcgcac gtcaccgcag catcgtcatc agctccatgg gagaacgatg ctgctttatc
    51241 agatcacgca tcacccgcat atgcggtgcg gagtaagaat aaaacgcctg atagcccgca
    51301 caaagcacgc tgtctggcgt ctcctggcga gcgtgcgggc acaggcgatg ccagtcgcac
    51361 gcctggcagt cacggcgcgg ttgcgggttt tcgctgccac accatttttg taaggtggcg
    51421 tcaaaaagct caatcgagat acgactgata tctgcccgca cccaacactc gaacagggca
    51481 attaaaaatt tcccccacag ctctgctgtc accgaagcgg ccgtcagatc gccccggtca
    51541 tcaaattcaa gatagggaat gagctgtacg gagcggatgc cccgctgttt gaaattgtga
    51601 taaaaatcgg caggttgcag agcatccttg tactctacaa ccagtttatg cctgtcctct
    51661 tcagcatcaa tgacctgact tcctgtcatg cgactctctc aataaatacg ataagttgat
    51721 gtatatgcgt tacagcaaac agtattgcga gagataacac cgcgagggag atcaaaaatt
    51781 tgtcacggac tacgtgaaat tgggaaaaat cgctattcgt gacatccatt aaattgcgat
    51841 tacgggtgta gtgccagagg atcagcgcca cgatggcgag aatgccaatc gaaatccaga
    51901 ataacatgcc cgcctggtgc cagttgtgtt tgatagccaa cgccatcaac gcgccgtagc
    51961 ccagcatggt acgaaaccac gccagtgatg tgcgctccgg ctgcagtcct gggtcggcaa
    52021 tgcgtctggc tttgcggcta tccggcatac aacaccagtc ccataacgat caccgccacg
    52081 accatcaata ttaagctaat gattaataag ctgttggtgt agggcaggtc ctctttcaga
    52141 cgcatcgctt tttcattgcg taaccagcgc aggtagccat acatcgctaa cccgccggaa
    52201 aacagacaca acaacaacgc cagcagttca cgaatgacgg gcgtggcgaa atctggcgca
    52261 agttgatcaa gcccaacccc cgccgccaga aatcctagtg cggtacggat ccacgccaga
    52321 aaagtacgct cattagccag cgagaagcgg taatcgggcg cttctccaag gcgggaaatt
    52381 ttcattgttg ctcctttgca atttcactaa cgctctacct ttagcggttt ttttttaaga
    52441 atttcgtccg cccgtttcct gatatgcttt cgccatccgc tacaaggagg tcagatgggt
    52501 atcatagctc aaaataaaat cagttcattg ggcatgctgt tcggtgccat cgctttaatg
    52561 atggggatta tccacttttc attcggccct ttttccgcac caccgccaac gtttgaaagc
    52621 atcgtggcgg acaaaacggc tgaaattaaa agaggtcttc tcgccggaat aaaaggcgaa
    52681 aaaattacca ccgtggagaa aaaagaggac gtagatgtcg ataaaatcct cgatcaaagc
    52741 ggtatcgcac tggccattgc tgcgctactt tgcgcattta ttggcggtat gcgtaaggaa
    52801 aatcgctggg gcattcgcgg cgcgctggtt tttggcggcg ggacgctggc tttccatacc
    52861 ttgctttttg gtatcggcat cgtgtgcagc attttgctga tattcctgat tttttccttc
    52921 ctgaccggcg gttcactggt ttaagcagcc tgaataatgc ccgccgggtg ttcatcagcg
    52981 ggcattatcg tcagaacttc tgttttttct cttccacttt cactccttgc gtcggtaggc
    53041 cggtgtcata gtcacgtacc accggcgaat agccatcttg tgggcgtgga cgaaatgccc
    53101 ccatccagcg cggcagtgca tctttacgcc acggacgcag gctccattgg taactgcgga
    53161 acggatcgcg aattttgtcc atgtaatcca ataaggcgtc atgcattttg ctgcgaacgt
    53221 ctgcaaaacg gatatcatcg atcaggttat gcatttcatt tgggtcatta cggcgatcgt
    53281 aaagttcatc actggtgaag aggttgagta ccagtttaaa gtcatccgtc acccagcaac
    53341 gcaccggaat aaaaccgcca aagctgtcat gctcaatctc gtagcggtta aattccacca
    53401 ttacaccgcg tggctctttc acggcaagga tattttcccc cggcagaatc tctggttttt
    53461 caatatctgc cagcgccatc attgtcggca gtaaatcgat atgactgact ggcgtatcga
    53521 cctgtcgccg ctccccttgc ggcgaacgga tgatcagcgg aatacgggtg atgtcgtcat
    53581 acatcgccgc tcctttactg atcagcttat gtgcgcccat catttcgccg tgatcggaag
    53641 tataaatgac ccacgtattt tcacgttgct ctggcgttaa ggcattgatg acccgtccga
    53701 tttggtcatc aacaaagtca ttacaggcaa aatagagcgg atggtgataa agcccgtcat
    53761 caccgactgg cgatggcatc gcctgcgccc ataagcggtg atgttccggt ttgttcgcca
    53821 ggccatcctg agctttctcg cccagatcgt agtaaaaatc agcgtatttc tctaaatact
    53881 ccaccggaca ggtgaacggg tgatgcggct catcatacga aaccaccatc aggaagggtt
    53941 cctcggcgcg cgcgggctgt tgcagaaaat ctactgcccg attgctgatg cggtgcgccc
    54001 aggtgaaggt ttcgtcgata tggttcgcct gtaaatcctc aacgctgttt aggccattgc
    54061 gccacaggct gatctctttt tccgtcagtt cgctaaggta gttcgcccca tcgaaccagt
    54121 aatcagcgtc ccactccggc ggacactcgc cagtgccgaa atagtcatga ccgtcgagat
    54181 gccatttgcc gatgtaacag gtgtgatagc cggcatcctt aaagtagcgc cccatagtgg
    54241 agatgttttt gcctggcgcg acgttgttgg tccacgggcc ggactggtta gcgtagatac
    54301 cggtaaatag tccggcgcgt gcaggcgtac aaaccggtga acaggtgtag gcggaattaa
    54361 agcgaatacc ttccgccgcc agactatcaa tattttgcgt attcagcggt ttaccgctat
    54421 agcaaccgac catattggtg gcctgggtat cggtcatgat gaacagaaaa ttggggcgtt
    54481 tcataggcta tccttcgcgt cgggagtaaa ggttacggct gggtcgtgac gatgccgcca
    54541 gctgtcaaaa atcaggtaga caatcactac ggcggcaatg aaataactga tcatcgctaa
    54601 ccagcgcgta ccgtagccgc cgaattcagc cagcccggca tagacgccaa tcatcgcgaa
    54661 caggatgcca attgacgcga tcttgacgtt tctccacggt ttcatgtcca ccgcaaacgc
    54721 atctttgaag gtgaacggcg tggcgcgcgg tttgataaaa ccgatcacca gcatcacgac
    54781 cacgttgatg cagaacgtac aggccagcac atagaggaaa tggaagtcga acttcaccag
    54841 atagttgatg gtgatgtagc tgattatgcc aatccccatc gccacttttg ccgccagcgc
    54901 cgggatgcgc gggaagaaaa agcccataat gatgatggta accagcggca cgttgtaaat
    54961 gccgttgagc tgtttcatcc agctatacag cccctgcggc gcgttggcga tccacggcgc
    55021 gaccagcacc gaaacgatag cgataaagaa accaaatttg cgcccgacgg tgaccagctg
    55081 ctgcggctcg gcattctggt taatgatgcg acggtaaata cccatactga ataacgtact
    55141 ggcgctattc agaaagccgt tgaaggtact gatcaccgca ccaaataaca ccgcgccgaa
    55201 gaaacccacc attggcactg gcagaacgtt attgaccagc gtcgggtagg ccatgtcggc
    55261 tttcggtaaa tcctgataca gatgaaacgc aatcaaccct ggcagcacca gtaccagcgg
    55321 gtcgagcatt ttcagcaccg ccgttaacag cgcccccttt tgcccttccg ccaggctttt
    55381 tgacgccagc gtgcgttgca cgatgccctg atttgtacac cagtaaaagg tgttcaccag
    55441 aatcaaaccg gtaaatgccg cgccaatcgg caagggatcg gtcgggccac cgattgagtt
    55501 taatttctcg gcgtgaacgg tggtgagttg ctcaatgcct tgcataaagc tgcccttgcc
    55561 catcgcgatc aggccaaata ccggcaccat caacccgccg ataaccagcc caataccgtt
    55621 gatggagtcc gccactgcca ttgcgcgcag tccgccgatc accgcataca aaattcccgc
    55681 cagaccaagc aaaattacca gtagccagat agccgcaccg tgagaaatct gtagcgattc
    55741 cccgacgtga aacaggctgt tgagcgccaa cgcgccggag tagagaacaa tcggcagaaa
    55801 gcagacgccg gtggcaatga ggaagcagaa gtcgatgata atacgcgtcg ttttatcata
    55861 acgttcctcc agaaaatcgg ggatggtggc aatgccgcgc ttcagatagc gcggtaaaaa
    55921 gattagcgcg aggaagatca gcgtcaccgc tgaagtcact tcccagccca tcaccgacat
    55981 gccgcttttg taggcctgcc cggaaaggcc gaccagttgt tccgtggaaa ggttggttag
    56041 cattaacgaa gcggcaataa ccggcgcttt tagtgaacgt ccggcaagaa aatagccttg
    56101 ttgcgatccg gtatctgttt tgcggacctt ccaccaggtg atcaccgcca ccagcagcgt
    56161 aaaaccgaca aaactcaaga tttgtaacga attcatctca tagcccttat ttatgtttat
    56221 gtcccggacg catgaatata gggagaggta aaatgcgtcg ggtcaggcag tcagattctt
    56281 aaaatacaaa cgtcgtatcc ctgaacggat tgttttctgt taagttcagg ttgtgagcat
    56341 aattcgccgg agggatatta aaatgaatgg aaaattgcaa agttcagatg taaaaaacga
    56401 aactccgtac aatattccct tattgattaa cgaaaatgtg atctccagcg gaatttctct
    56461 gatctcgctc tggcatacct acgccgacga gcattaccgg gtgatctggc cgcgggacaa
    56521 gaagaaaccg cttatcgcca actcatgggt tgcggtgtat accgtacaag gatgtgggaa
    56581 aattctttta aagaatggcg aacaaataac actgcatggc aactgtatta tattcttaaa
    56641 gccaatggat attcactctt atcactgtga aggtttagtc tgggaacagt actggatgga
    56701 atttaccccc accagtatga tggatattcc cgttggtcag caaagcgtta tttataatgg
    56761 cgaaatttat aatcaggaac tcaccgaagt tgctgagtta ataacttcac cagaagcaat
    56821 aaaaaataat ctggcagtcg cttttctgac gaaaattatt tatcagtgga tttgtcttat
    56881 gtacgcagat ggtaaaaaag atccacaacg gcggcaaatt gaaaaattaa ttgccacttt
    56941 acatgccagt ctgcaacaac gctggagcgt agctgatatg gctgccacga tcccctgtag
    57001 cgaagcctgg ttgcgtcgtc tgtttttacg ctataccggc aagacgccga aagaatatta
    57061 cctcgatgcg cgtctggatc tggcgctatc gctattaaag caacaaggaa actcggttgg
    57121 cgaagtcgct gatacgctca acttcttcga ctcctttcat ttcagcaaag cctttaaaca
    57181 taaatttggt tatgcgccgt cagccgtgct aaagaatacg gaccagcacc caacggatgc
    57241 cagtccacac aattaacgta actctggcca gtacgctttg ttggcctcca gcaactcatc
    57301 cagaatagct ttggcgaccg aggcgctcgg taccgttttc gacaacgtaa tcgcctgcca
    57361 caggtgctga tatgaacgct gttcccaggc gtccacgacc agtttttcca ccgccacttg
    57421 ctgactcatt aaccctttct gaaactgcgg gatatcgccg accaccagtg gttcaggccg
    57481 tagtagcgca cgcgcatctg tttgcgatct tgcagcccaa caatttgcgc catccggctt
    57541 tcaataccga ttggcatgtc acagatgttg aggattttcg cattcgggcg cagacgacgc
    57601 gtggcttctg cgacaattgc tgccgggttg gagtagttga gcatccaggc gtttggtgaa
    57661 tatttttcca tataatccac cagttccagg acgccgccaa tggaacgcat gccgtacgct
    57721 attccgccgg gtccgcaagt ttcctgacca acaacaccgt ggcgcagtgg gattttttca
    57781 tccagttcgc gcatcgggta cttgccgacg cggatgtgcg ccatgacaaa atcaacgtcg
    57841 ctgaacgcta cttcaggatc ggtggtgtaa ctaaacgcaa tgtccggcgc tttttcttta
    57901 aggatgactt tacaggcttc ggcaatcact tcctgccgcg cgccatcgtt atcataaaat
    57961 ttcagtgcac gaagcgggaa acggtcctga ttcgccagga gcatcaacac gatgcctggc
    58021 gtaaaggtgc ttccaccgcc tgcgacaacc actgagaatt tggtcattat actgcctccg
    58081 taatggcaac attttctgct gattgatgag aattaattaa gctatcgagc tgttcacgca
    58141 attgggatac atgcagacca atgatcacct gaatggcatc gccactacgg aagacgccgt
    58201 gcgctcccag ctttttaaag acttcgtcat ccagcgtttg tgacatgtca tgcagtgcaa
    58261 tgcgtagacg cgtcgcgcaa ttgttaatgc tggagatatt gccgaccccg cccagggctt
    58321 gcagaatacc ggcagcctga tccagctctt tttttggctc tgccgcggtg gtttggcctc
    58381 gcgaggcttt gtattcggct tttgagtaga gtttcacttc cgcatcttca cgtcccggcg
    58441 tgcacatgtt gaactgcaga atcagggtgc ggaaaaccac gaagtacagc agggtaaagc
    58501 acaacccaat ggcgatttgg gtcagcatca tatccgcgtg gttgctgaac atcgggatcc
    58561 agttttgcgg taaaacctgg tcaatcagac ctccgcccat gttgcccacc acaccaaaga
    58621 gatacatcac ggtcgacatt gaggccgcca gcacggcgtg taccgcaaac agcaacggtg
    58681 aaatgaacag gaaggtaaat tccagcggtt cggtaattcc caccagcatg gcggttaagg
    58741 ttgcgggaat cagcaagccc gccactttta cccgattttc cggtgcggca gtgaagtaca
    58801 tcgctaaaga aatgcccacg gcaccaaaga tttttgagtt accgtgcagg gcaaaaccgc
    58861 cttccgggaa caacgatttc agcggctcgg cgctcagact gaactcttgc agatgctgcg
    58921 cccagtacat ctgaatgccg ccttcaacag ctgccggacc aaagataaac ggtccgtaga
    58981 tgaagtggtg taaaccggtt gggatcagaa tacgttcgag gaaggtgtaa acccacaccc
    59041 caagcgcacc cgccgaacgc aggaacgctt gcagagattc aatccccatt tgtacttttg
    59101 gccagccgag caacgtcagc caggcacagg ggatcatcac caggaaggcg ataatcacca
    59161 cataagacgt cccctggaaa atgccgagaa aaaccggcag ttttttatcg aacagacggt
    59221 tatgcagcgc cgtcacaatg ccggaaatga taattgcgcc gataatgctg gtatcgaggg
    59281 ttttaatccc ggccatcatt gtcagaccgc tacctgccac cgcgtcctga gtgaaatcga
    59341 cgccgaagta gcttccccag gtcattccca tcgcgttgat gaaatagttc caggtcagga
    59401 aactcaccat caccgccaga caagcacgcc cctgcgcttg cttagcaagg ccaatgggta
    59461 aaccgacagc aaaaatcagc ggcatattac ggaacaccgt ccaaccgccc tcttcaataa
    59521 tgtgtacgat ttgcgcgaat aaactgttcg gatcggtcag tgattccccg acaaacatcg
    59581 ggttttgcag caagatggca agacccacca caatcccggc gaagggaaac agcagcactg
    59641 gcgtgaacat cgcgccgcca aagcgttgaa tttgactgag catttccaca tcctcttttc
    59701 tcaattctgt aagtaggaag cctgattggt tatgcggcag gcctggagcg aggataggaa
    59761 gatgcgatcg gcagaacatg acgacccaat gggattcgtg atcgctttca tgcttttcgt
    59821 tgggcggtag agatctacgt tttcgcgaaa aagtagacat ctgtgagcac aggtacacag
    59881 catcgaacac atctttaaaa aaaagatgtt ttttcaatcg attaagcaga acttgtgggc
    59941 gcattacccg tgcttgcagg caaaaaagag atctagagat gatctacaaa agcattgcgg
    60001 agcggttaag aattcgactt aattccgcag atttcacgct aaatagcctt cttcccggtg
    60061 aaaaaaagtt ggcggaagaa tttgcggtat cgcggatgac catccgtaaa gccattgacc
    60121 tgctggtagc gtggggattg gtggtccgcc gccacggcag cggcacttac ctggtgcgca
    60181 aagatgtact gcatcaaacc gccagcctga ccgggctggt ggaggtgtta aaacggcagg
    60241 gaaaaacggt caccagccag gtgctgattt ttgaaatcat gcctgcgcct ccggccattg
    60301 ccagccagtt acggattcaa atcaacgagc agatctactt ctcccgtcgc gttcgttttg
    60361 tggaagggaa accgctgatg ctggaagaca gctatatgcc ggtaaaactg ttccgtaatc
    60421 tttcgctgca acatctggaa gggtcgaagt ttgaatatat tgaacaagag tgcgggattt
    60481 tgattggcgg taattatgaa agcctgacgc cagtgctcgc cgatagactg ctggcgcggc
    60541 aaatgaaggt agcggaacac acgccactgc tgcggatcac ctcgttgtca tatagcgaga
    60601 gcggggagtt tttgaattat tcagtgatgt tcagaaatgc cagcgaatac caggtggagt
    60661 accatttacg gcgactccac ccggaaaaga gttaaccgat actccagaag agcaccgcca
    60721 gtaattgggg ggtgataatg cgcaggaaca tcactaacgg atagacagtg gcgtaagaga
    60781 gcgccgccgc accgctggtt ggatgaagat tattagcaaa cgccagcgcc ggaggatcgg
    60841 tcatggaacc tgccagcatc ccgcacatgg tcaggtaatt cattttggct aacatccgcg
    60901 ccagaatgcc aacagtaatc agcggaacgg cggtgatcag ggcaccataa ccaatccagc
    60961 ttagcccttc gccattgacc agggtattca caaaatctcc accagatttc agaccaacga
    61021 ccgagaggaa cagcacgatc cccagctccc gcagcgcgag gttggcactt ggcggcataa
    61081 accagtacag cttgccgata ctgccgatac gcccgaggat caacgccata atcagcggac
    61141 cgcccgccag ccccagtttc aacgcggccg ggaacccagg cacaaagacg ggaatagaac
    61201 ctaacaatac gccaagcccg atgccaataa acactggcag catctgaacc tgttgcagtt
    61261 tttgctgcgc attccccagc acattggcaa cggcatcaat tgcggacgga cgccccacca
    61321 gattgaggat atcgccgaac tgcaggctga tatcgccact ggcgaccagt tcgaccccgg
    61381 cacggttcag gcgcgagata acaacgtcat agcgttcttt aaagtgcagg tcgcgaatac
    61441 gttttccgag cacgttttca ttggtgacca ccacacgctc gacgcgcaaa tcagtgcctt
    61501 tcgtggacag cgaagtatcg acctcctgac caatcaccag ttgcgcatta tgtaaatccg
    61561 ctggctgccc caccagatgc agcaaatcgc ccagttggat aatggtatct ggcgaaggaa
    61621 cttttagggt ttcttcgcgt ttcagacgcg agcagataat tttgtcgccg ttgagaatcg
    61681 gtacatcttt aatggctaaa tcatgcaggt tagggttctc aacgcgaata ttgatagtct
    61741 tgatcagcgc gccgccattg gtgcgtgaag actcgtgctg ctgagcttct gtctcgacat
    61801 tgacgcggaa aataacccgc aacatccaca tggtgaacaa aatcccgcaa atgccgaatg
    61861 gatacgccat cgcgtaactc atccccatct gatcgaccat ttccattggt gtacccaagt
    61921 cgcgcaaaat ctgctgccct gcccccagcg ctggcgtatt ggtaaccgca ccggagaaaa
    61981 tccccagcac taccggcagt ggcatatcaa acagtttatg caggatggcg gtaaccagac
    62041 caccgatgat gacgatcaga acagcaaaca ggttgaggcg taatccggag acgcgcaatg
    62101 aggcaaagaa gcccggccct acctgaatcc cgatagtata aacgaacagg atcaggccaa
    62161 attcctgaat aacatgcagc atatcgctac ttaatgtcat ccccgcctga gaaacaaaat
    62221 ggccgacgat gatcccacca aacagcacgc cgccaatacc taatcctatg ccgcgaaatt
    62281 tgacattgcc gataaacaaa ccgacgactg ccaccaaagc cagaatactg accgttaatg
    62341 ctatatcact catcgtccat ttctcttgag ataacatttt taattgctac ggattatggc
    62401 tgaatcggtg aagcagattt gccttatagc gcacattatc acgggaaaga acgtgccgaa
    62461 atatcttaaa cagtcgaaat ttaacgcatt gttgagtaag taagacaaaa aaagccccac
    62521 cagaatggcg gggcaaagag aatagctagt tagctattta acgcgggacg ttcgctgata
    62581 gcgatacgct gcgctgcgat gggttcaggc tcattacgaa ttaaatcaat atgcagtaaa
    62641 ccgtttacga aggttgcgcc agagacttcc atattttcag ccagcgtaaa gctcaggcta
    62701 aatggctgat tcataagccc ttgatgcagc cattttttct cttcttttgg ctgctccggc
    62761 gtgcctttta cgctcaggcg cgtaccttcc agttgaatct ctaaatcttc ctgacggaaa
    62821 cctgccagcg caagggtaat gcggtagtgg ttatcgtcgc ttttctcaat gttgtacggc
    62881 gggaagctct ggctttcacc ggcgttttgc agtgcgttgg ccagtttgtc aaaaccgatc
    62941 cattgacgca tcagtgggga taaatcgaag ttacgcatag tcatttctcc ttctaagaag
    63001 cgagtaagta cctgcaaatc cgaagattcg catatgctcc ctaacggcga gcatggagat
    63061 gtcaggccgc gccaggcggc cttagggaat tagttgattt cgatacggcg cggttttttc
    63121 gcttccggaa tcacgcgttc gagatcgata tacagcaaac catttaccag gttagcgcca
    63181 cgaacatgaa tgttctcagc taactggaat ttgcgttcaa agttgcgttc agcgatgccc
    63241 tggtacagat aggtgcgctc tttttgttcg tcggcgtgag cacctttcac cactagcaga
    63301 ttatcctggg cggtaatttc cagttcgctc tcagcaaaac cagccacagc gatagcaatg
    63361 cggtaatggt tttcgtctac cagttcaacg ttatacggag ggtagccgcc attactctgg
    63421 ctctggttgt tttctaagtg gttaaacaaa cggtcaaatc caatagcaga acggtaaagc
    63481 ggggataaat caaagttacg cataatcaat agctcctgaa atcagcgaga atgtaagacc
    63541 ttccacaatg gacaggtcag gtagccagaa cacccatcag gcgcgtcctc atcggctacg
    63601 atgtaaaaat gggtctggaa atgaactttt caagccttat cagcggactt ttttttgcag
    63661 tttatggtct attgcataga ctgagggggc agcacgaaac gttaaagtgc gatagccgcc
    63721 acagtgcgac gtaatgatga tgttattttc agcaagggat cgctataact catcaggcca
    63781 acacccacga tcaacagatg aatttattat gataagaaat gtgttgttag cgttcatgat
    63841 atgcagcgga atgacattac tcggaggatg ctccagcgtg atgtcccata ccggagggaa
    63901 agaaggaacg tatcccggca cccgcgccag cgcaaccatg attggcgacg atgaaaccaa
    63961 ctggggcact aagtcgctgg cgattctcga tatgccgttt accgctgtga tggacacgct
    64021 tttactgccg tgggatgtgt tccgcaaaga cagctcggtg cgctcacgcg ttgaaaaaag
    64081 cgaagccaac gcgcaagcca ccaatgcggt tattccaccg gccagaatgc ctgataactg
    64141 atttagcgat cagtttcggt gatccacagc tggccacctt ccatccacgc cagccattga
    64201 ccatccggcg agaagacctc ggcgtccgca gaaggtggat tggcgtggtt ttcggttaaa
    64261 tactcaacct cgccgctttg cgcatgggca caagcaattc gattatccag cacaaagccc
    64321 aaccattctc ctgacggatg ccagttaaat gcagactgaa tatccgtttt gttatgggtt
    64381 aactggcgcg gctcgccgcc ctgtggcgag ataagccaca gttgcacaat gccgttatca
    64441 tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac agcgcaccca gtggcgcggg
    64501 acgttgacta accccggata agcccgatgg tgggtaaagg ttaaacgtcg ctgcacgacg
    64561 ccgcgcggtg gcgcgggcag ggtggtttcc gttccactta acggcgcatc acctgccgct
    64621 ttccagccag cttcatcttg cggtaactca acgataaaca gctccggcac tttctcgccc
    64681 tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat ttcctaccca tccttcttca
    64741 taagcacgat tgatttcatc gctgccaggc tgcggcgtgg gcgtggtttt actcaccagc
    64801 acgcaccagt ggctaccgct gtattcacgc ggatgctgct tttgtacgtt gaccgggcca
    64861 aacggcgcag caacaccgac gtttcgcaaa tccagcgccg gatcgagttc atgcattaca
    64921 tggtcgttat aggtaaagct caccctttca ccgttcgggc taaagacatg cacatggctg
    64981 ccgccgcgca gcgctcctgg catatacggc gcggtaatat ccattgcatc gagattgctc
    65041 accttgccgc cttcagcaat gactccgcgt cgatgatgga aatcgtaatg ccatgtttca
    65101 tcaggatttt ccgggccatg gatgaaaaca tatttctctg acttcggatg aacggtcacc
    65161 acgccgacgt gtgcgccctg tgacgcgcga tagataacct cgacctcgcc ggtatggata
    65221 ttcacacgct caatggtctc gccggtaaac gacgccccag aaggacgcac gtcaaatacc
    65281 agccactggc tgtcgggcgt ccaggtattg gtattggtga gcaggtgatt acggggagca
    65341 aaggttatct gtttcatggc gcggtcctga tacaaaacgg gtcccgccat catacttcac
    65401 aaggacttca ctttcagaca atagtctttt gccatcgtga tgaatgtgag tgaaaagatg
    65461 gaacatttcg acgtggcgat tattggcctc ggcccggcag gatcggcgtt ggcgcgaaaa
    65521 ttagcaggca aaatgcaggt gatcgcgctg gataaaaagc accagtgtgg tactgaaggt
    65581 ttcagcaaac cttgcggcgg tctgctggca ccggacgcgc agcgatcttt tattcgcgat
    65641 ggactgacgc ttcctgtcga tgtgatcgcc aacccgcaga ttttcagcgt caaaactgtc
    65701 gacgtcgccg catcgctcac gcgtaactac cagcgaagct atatcaatat taatcgccat
    65761 gctttcgact tgtggatgaa atcgctgatc cccgccagcg ttgagattta tcacgacagc
    65821 ctgtgtcgaa aaatctggcg tgaggatgat aaatggcatg tcatttttcg tgcagacggc
    65881 tgggagcaac atattactgc ccgctatctg gtcggtgccg atggcgcaaa ctcgatggtg
    65941 cggcgacatc tctacccgga tcatcaaatc cgtaaatatg tcgctatcca gcagtggttc
    66001 gcagagaaac atccggtgcc gttctactcc tgcatctttg ataattcgat aactaactgt
    66061 tattcatgga gtatcagcaa agacggttat tttatctttg gcggtgctta tccaatgaaa
    66121 gacggtcaga cgcgtttcac gacgctgaaa gagaaaatga gcgcctttca gttccagttt
    66181 ggtaaggcgg tgaaaagcga aaaatgcacg gtgctgtttc cctcacgctg gcaggatttt
    66241 gtctgcggta aggacaacgc ctttctgatt ggcgaagcgg caggatttat cagcgccagt
    66301 tcgctggagg ggattagcta tgcgctggat agcgcagaca ttctgcgttc ggtgttactg
    66361 aagcagccag agaagctcaa taccgcttac tggcgcgcca cccgcaaact gcgtttaaaa
    66421 ctcttcggca agatagtaaa aagccgatgc ctgaccgcac cggctttaag aaagcggatt
    66481 atgcgcagtg gtatggcgca tattccacag ttgaaagatt agccaacgcg cttcacatcg
    66541 cccaccagca ggatgtaaga gagcgcgcca atcaacgcga cggcggagat ataaaccagt
    66601 gcaggtgcga aaccgtaacc ctgcgccagg taccccacca ccagcggaac ggtgatgccg
    66661 cccagaccac cggcgaagtt aaacacgccg ccggttaaac caatcaggcg catcggtgcc
    66721 agagaagaga ccagcgacca ggtaatcgaa gcaaaaccgt tgccgaagaa tgccagcgcc
    66781 atcaggcaca taatcatcat cggatcgttg gtgtagttag cgcccataat gcaggtggag
    66841 atcagcaagc cgcagataat cggcgtttta cgcgcaaagc ccagtgaaaa gcccttacgt
    66901 accagcagat ccgcgaccca gccagagagc aggacgccga caaacgccgc gaggaatggc
    66961 accgtggtca taaagcctgc tttcagcgcc gtgattcctt tttcctgggt taaatagttc
    67021 gggaaccagg ttaagaaaaa ccacagtgta gaagccaccg caaattgccc aagataaacg
    67081 ccgatcagtt tacgatggaa caccagtttc cagtctttgg ctgttaacgg ctgacgcgcc
    67141 tctttcttca ccggcgcatc accatccacc agaccgccgc catcacgaat gtaatccagt
    67201 tcagctttgc tgataccttt ggtcaggcgc ggcggctgat aaaccttaaa ccaaatcagc
    67261 gaccagataa tgccgatacc accagtgaca atgaacaccc agtgccagct caacatctcc
    67321 tgaatccaga tcagcagcgg agtcaaaaac gccagaccga caaactgacc agacgtataa
    67381 aaaccaacgg cggaagcgcg ttcatgttcc gggaaccagc tggtcaccat ccggttattg
    67441 gtcgggaaag cgggcgcttc gaaaataccg gttatcgcac gcagaccaat taatgacatt
    67501 aatcccgtgg caaagccctg gaataaagtc gccactgacc aaccaaatat cgcaataaaa
    67561 taagtcacgc gagaacctac gcgatctaaa aaccaaccgc cggggatctg gcatagcgta
    67621 taaagccagg cgaaggccga aaatacatag cccatttccg ctttcgtaat gccgaactct
    67681 tcctgaatat gggcggaagc cacggccagg ttagcgcggt caacataaca aatgaccacc
    67741 gtaataaaga tcatcaccag cgtcagataa cgccgacgcc ccggctttgc tgcattaacg
    67801 ggaatatcca tagcgagctt tctccagatt ttgggcatag cgaagccgct caccatgccc
    67861 tgtaatttac agagggttat atttttgtat tgctgtttta gtgcccgatg aggggcttac
    67921 gtggcaggaa ttaccactct gctacgctgt tatcttcatg acgccagagc ggattacgcc
    67981 agtccggggc atttttactg aactcaataa ctttagcttc gtcgatttcc acacctaagc
    68041 ccggtttcgt taacggttta aagaagccgc caaccatact gaagtcttct ttgtttttca
    68101 caaagtcgag taactccgcg cctttgttgt aatgaatgcc catactttgt tcctgaagta
    68161 ccgcgttata ggaaacaaag tcgatatgca ggcaagccgc cagtgcaatc ggtccgagcg
    68221 gacagtgcgg cgcaagggtc acatcatagg cttctgccat tccagcaatt ttgtagcatt
    68281 cggtaatacc gcctgcatgg gagagatccg gttgcagaat cgaaataccg cctgcctcca
    68341 gcacgcgttt aaaatcgaaa cgtgagaaca tgcgctcacc tgccgccagt ggaatatgcg
    68401 tttgtgccgc caatttcggg tagtattcgg cttgttccgc cagcaccggc tcttcaataa
    68461 acagcgggcg atacggctcc agttctttaa tcagcacttt cgccattggc gcgctgacgc
    68521 gaccgtggaa atcaagacca aactcaatct gattgccaaa agcttcacga atttgtgcca
    68581 cggtgttgac tgccgcatct accgcgcggg agttatcaat tagccccagt tcttcacaac
    68641 cgttcagttt gaaggtatcg aagccgattt cgcgcagggt tttaatgccg tcgataacat
    68701 ccgccggacg atcgccgccg acccaactgt aggctttaat tttgtcgcga accaggccgc
    68761 ccatcagttg ccagaccggc gcattcagca ctttgccttt gatatcccat aacgcctggt
    68821 cgatcccggc aatggcgctc atcaggattg gaccgccacg ataaaatccg gcgcgataca
    68881 tcacttgcca taagtcattg atgcgcgaag gatcctgacc aatcaaatag tcacccagct
    68941 cgtgaactgc cgcttccacc gtacgggcgc ggccttcaat cacgggctcg ccccaaccga
    69001 ccacgccttc atcggtttca attttcagga acatccagcg gggaggtaaa cgatacgtgg
    69061 taattttggt gattttcatt gcactgcctc tcgatacgcc ttaacaaatg ctgctgcctg
    69121 ctgcgcggtg cgttctacgg actgcccggc gcgatagaga tcgctgccta agcccgcccc
    69181 agcacaacct gcgtctatcc actgcgccag gttttctggc gtcacgccgc caacggcaaa
    69241 gactgcgatg tccgatggca ataccgcttt taacgctttg atgtattgcg gaccaaaagc
    69301 cgatgacgga aatattttca gcgcctgcgc gcccgcttcg agcgcggtaa aggcttcggt
    69361 cgccgtcgcg cagccggggc agacggtcat gccgtagccc accgcacggc ggatcacttc
    69421 actatggata ttgggcgtaa cgatgagctg acaacccatc ctggcgagcg catcgacctg
    69481 ttcaggtttc agtaccgtac ctgcgccaat caacgccttg tcgccatacg catcaacgat
    69541 ggcgggaatg ctttgctccc attgtgggga attcagcggg atttcaaccg cgtcgaaccc
    69601 ggcgtcaatc accgcgccaa catgcgccag cgcctcgtcg ggcttaatgc cgcgcaaaat
    69661 ggcgatcagc ggaagtttag tttgccactg catgagcgat gctccttata ccagcctgaa
    69721 atgccgtgtc gcccgccacc gccgtcacgt cgcaacccat cgcctgaaag gcttgctggt
    69781 agcgcgcggt cagcgatgtt ccggcgacaa gggtgatggc gtgttgatgg gtcacatagt
    69841 cgcgcatact ggcaacctct gcgccaatca acaaaccaga gagaaattcg ctgacctgtt
    69901 cgcggggaag tgttcccagc acatgcgagg cgcgaacttc aaaaagctgc ggcaatatgg
    69961 cgggcgcatt aaggccgcgc tcaaggccag ccgcgaaggc atcggcagag ttttcctgcg
    70021 gcggcaaacc tgcgccaatc aatgagtgat ttaacagtaa atgatgtaat tcaccggtca
    70081 tcacggtgcg aaaatcgttg atttgctggc tatcggcctg cacccatttg caatgcgtac
    70141 cgggcataac ataaagagag gaaggagcca gagttcgcgc gccgatcaat tgtgtttctt
    70201 cgccgcgcat cacattgtgg ttatcgtcat gagagacgca taatccggga ataatccaga
    70261 tattgtcgcc aacagacgtt aattgttcgc caatagacga aaaacgggca ggaacagata
    70321 aatacggagc aactttccag ccgacattgc tgccgaccat tcctgccatt accactggcg
    70381 tattctcttc acgccagtcg gtcgtgactt ctgctaacac cgcagccgga gattttccgt
    70441 tcaggcgcgt gacgcctgct tctgattgcc tgctctccag gcagtggtcg ccctgataaa
    70501 gccaggcgcg aagattggtc gatccccagt caattgcgat gtagcgagct gtcatgtgat
    70561 ttcctttaac cttcgtgtcg agctggcgat catggtaagc gccgcctgct ctgccgcatc
    70621 gccgtcctga tgccgtattg catcgaacag cgccttatgt tcctggagcg tttgcggcat
    70681 gttggcctca tcgcccatcc aggttcgttc aaaaaccgcc cgctgcaacg aactgatcgc
    70741 aatgctaagt tgctgtaaca ccgggttatg taccgactgc agcaccgcct cgtggtagcg
    70801 aatatccgct tcgttaaacg cttcgcggtc ctgattgttg gcaatcatct cgttcagcgc
    70861 cgattcaatc tgcgccagat cgctggaagt cgcgcgctct gctgcccaac gggcaatcgc
    70921 cggttccacc agatttcgca cttcgctcat ggcactgata agccgtgggt cgtagtcgtt
    70981 ttccagcacc cattgcagta cgtcagtgtc gaggtaattc cactggttac gcggtgccac
    71041 aaacgcgccg cgataacgtt tcatttcaat cagccgtttc gccatcaacg aacggaacac
    71101 ctcacggatg atgttgcgcg aggttgcaaa ctcttcacag agctccgcct cagccggaag
    71161 tggcgagcca ggtacgtatt tgccgtgaac gatctgttta cccagcgtaa tgacaatgcg
    71221 atcggtttta ttgagagtca tggagagtcc ttgtgctctg tatgttcttc tctactttac
    71281 cccgatcgat gcataacgcg gcaactttgt agtaccagcg tgatgacgtt cgcgtttgcc
    71341 gtgtgcgtaa tgtagtacaa acttatattg ttgtactaca atttagatca caaaaagaac
    71401 aatgcataaa aaaatgacat gcgtcgggca gaaatctgaa aagggatatc aggcgctaaa
    71461 caggagggaa agaagagtat gctttcaacg gcttagctac tcgtttaaag gattaatcat
    71521 gaagttgaat tttaagggat tttttaaggc tgccggttta ttcccactgg cgctgatgct
    71581 ttcaggctgt atctcgtatg ctctggtttc ccataccgca aagggtagtt caggaaagta
    71641 tcaatcgcag tcagacacca tcactgggct atcgcaggca aaagatagta atggaacaaa
    71701 aggctatgtt tttgtagggg aatcgctgga ttaccttatc actgatggtg ccgatgacat
    71761 cgttaagatg ctcaatgatc cagcacttaa ccggcacaat attcaggttg ccgatgacgc
    71821 aagatttgtt ttaaatgcgg ggaaaaagaa atttaccggc acaatatcgc tttactacca
    71881 ctggaataac gaagaagaaa aggcactggc aacgcattat ggttttgcct gtggtgttca
    71941 acactgtacc aggtcactgg aaaacctaaa aggcacaatc catgagaaaa ataaaaacat
    72001 ggattactca aaggtgatgg cgttctatca tccgtttaaa gtgcgatttt atgaatacta
    72061 ttcacccaga ggcattccgg atggtgtttc cgcagcatta ctgccagtga ctgttacgct
    72121 ggacatcatt actgcaccgc tgcaatttct ggttgtatat gcagtaaacc aataatcagt
    72181 aagcgggcaa acgcgtttat gctgtttgcc cgtccactga ttaattcagc acatacttct
    72241 caatagcaaa cgccacgcca tcttcaaggt tggatttggt gacaaagttc gccacttctt
    72301 tcaccgaagg aatagcgtta tccatcgcca caccgacgcc tgcatattca atcattgcga
    72361 tgtcgttttc ctgatcgcca atcgccatga tttcttccgg tttaatacct aacacgtcgg
    72421 ccagtgattt cacccccgta cctttgttaa cgcgtttatc gaggatttcg aggaagtacg
    72481 gcgcactttt cagcacggta tatttctctt tcacttcctg cggaatacgc gcgatagcct
    72541 ggtcgaggat ggcgggttca tcaatcatca tcactttcag gaactgggta ttggggtcca
    72601 ttttctccgc ttcgcagaac accagcggaa ttgtggcaac gaaggattca tgcaccgtgt
    72661 agtagctgat atcacggttg gcggtgtaca gcgtggtgcg gtccagggcg tggaaatgag
    72721 aaccgacttc gcgagagagt ttttccagga aacggtagtc gtcatagctg agagcggttt
    72781 gcgccacggt gctaccatca gcggccttct gtaccagcgc gccgttataa gtaatgcagt
    72841 agtcgcccgg cagttccata tgcagctctt tcaggtaatt gtgaacacct gcataagggc
    72901 gacccgtcgt tagcacgaca ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa
    72961 cggcgggtga aatggtgtga tcgggtagca gaagggtgcc atccatatcg atagcaatga
    73021 gtttgatagc catgagttcc ccaggtagat tagttcctga cccatgctaa cgcgattccg
    73081 ctcaaaaatc agtacaacac ccgagggaaa agggggatgc aacgcgggtt gcatgctccc
    73141 catttgctta gcggaagagt ttccctttca gcagttccat gcctgcggaa agcagatcgt
    73201 tattggcttg tggagacact tcaccttgcg gtgagagcgc atcaataatc ttcggcaatt
    73261 gttctgccag taaactggaa gctgtactgg tatccacgcc aagtttttgt ccaagatcgg
    73321 acaccgcatt tgtgccgagc gccgattcca gttgctcgcc actaaccgat tgattgccct
    73381 gttgattact cagccaggtt gagagaatgg cccctaagcc gccactttgc agtttttcca
    73441 gcagcacctg aatgccgccc tgctcctcaa cccaacttaa aatagcctga tatttccccg
    73501 catcgccttt cagaaaggca ccgacaactt catcaaaaag ccccatgata atcacctgta
    73561 aagcgttacg tgttgaccca aaaagtatag atttgcggga tgataattgc ggattgcaga
    73621 aataaaaagg gcggagatga tctccgccct tttcttatag cttcttgccg gatgcggcgt
    73681 gaacgcctta tccggcctac aaaatcatga aaattcaata cattgcaaga ttttcgtagg
    73741 cctgataagc gtagcgcatc aggcacgctc gcatggttag cgccattaaa tatcgatatt
    73801 cgccgctttc agggcgttct cttcaataaa cgcacggcgc ggttcaacgg cgtcgcccat
    73861 cagcgtggtg aacaactggt cagcggcaat cgcatcctta acggtaacgc gcagcatacg
    73921 acggctttcc gggtccatgg tggtttccca cagctgttcc gggttcatct cgcccagacc
    73981 tttataacgc tggatggaga ggccgcgacg ggactctttc accagccagt ccagcgcctg
    74041 ctcgaagctg gctaccggct gacgacgctc gccacgttcg ataaacgcat cttcttccag
    74101 caagccacgc agtttctcac ccagcgtgca gatacgacga tattcgccac cggtgataaa
    74161 ctcgtgatcc agcggatagt cagtatccac accgtgggta cgcacgcgaa caatcggctc
    74221 gaacaggttt tgttcggcat tggtgtggac atcaaacttc cactggctgc cgtgctgttc
    74281 tttgtcgttc agttcgctga ccagcgcgtt cacccagcgg gtaacggtct gctcatcaga
    74341 gaggtcggct tccgtcagcg tcggctgata gataagctct ttcagcattg ctttcggata
    74401 acgacgctcc atgcggttga tcattttctg cgtcgcgttg tactcagaca ccaatttctc
    74461 taacgcttcg ccagccaatg ccggtgcgct ggcgttggtg tgcagcgttg cgccatccag
    74521 cgcgatagag atctggtact gatccatcgc ttcgtcgtct ttaatgtact gttcctgctt
    74581 gcctttcttc actttgtaca gcggcggctg agcgatgtag acgtgaccgc gttcaacgat
    74641 ttccggcatc tgacgataga agaaggtcaa cagcagcgta cgaatgtgcg agccgtcgac
    74701 gtccgcatcg gtcatgatga tgatgctgtg ataacgcagt ttgtccgggt tgtactcgtc
    74761 acgaccgata ccacagccaa gcgcggtgat aagcgtcgcc acttcctgag aagagagcat
    74821 cttatcgaag cgcgctttct cgacgttgag gattttaccc ttcagcggca gaatcgcctg
    74881 gttcttgcgg ttacgcccct gcttcgcaga gccgcccgcg gagtcccctt ccaccaggta
    74941 cagttcggaa agcgccggat cgcgttcctg gcagtctgcc agtttgcccg gcaggcccgc
    75001 taagtcgagc gcacctttac ggcgggtcat ttcacgcgcg cgacgcgccg cttcacgggc
    75061 acgggcagca tcgataattt tgccaaccac gattttcgcg tcggttgggt tttccagcag
    75121 gtattctgcc agcagttcgt tcatctgctg ttcaaccgct gatttcacct cagaagaaac
    75181 cagtttgtct ttggtctggg aggagaattt cgggtccggc actttcacgg aaacgaccgc
    75241 aatcaggcct tcacgcgcat cgtcaccggt ggcgctaact ttggcttttt tgctgtagcc
    75301 ttctttgtcc atgtaggcgt tcagggtacg ggtcatcgcc gcacggaagc ctgccaggtg
    75361 agtaccgccg tcacgctgcg gaatgttgtt ggtaaagcag tagatgtttt cctggaagcc
    75421 atcgttccac tgcaacgcca cttcgacgcc aataccgtct ttttcagtgg agaagtagaa
    75481 gatattcggg tggatcggcg ttttgttctt gttcagatat tcaacgaacg ccttgatgcc
    75541 gccttcatag tggaagtggt cttctttgcc gtcgcgcttg tcgcgcagac gaatggaaac
    75601 gccggagttg aggaacgaca actcacgcag acgtttcgcc agaatttcat attcgaactc
    75661 ggtcacattg gtgaaagttt cgaggctagg ccagaaacgc accatggtgc cggttttttc
    75721 agtctcgccg gtaaccgcca gcggggcctg cggtacaccg tgttcgtaga tctgacggtg
    75781 aattttaccc tcgcgctgga taaccagctc cagtttttgc gacagggcgt ttactaccga
    75841 aacaccaacg ccgtgcagac cgccggacac tttataggag ttatcgtcga atttaccgcc
    75901 tgcgtgcaga acggtcatga tcacttccgc cgccgatacg ccctcttccg ggtgaatacc
    75961 ggtcggaatg ccgcgcccgt catcctgtac agagacagag ttgtcggcgt gaatggtgac
    76021 gataatttct ttacagtgac ccgcgagcgc ttcgtcgata gcgttatcta ccacctcgaa
    76081 taccatgtgg tgcagaccgg tgccgtcatc cgtgtcgccg atatacatac ccgggcgctt
    76141 acgcaccgca tccagccctt tcaggacttt gatactggag gagtcataag aattcgacat
    76201 caacgtttct cgctcattta tacttgggtt aatccgttat tttacccttt tccacggtaa
    76261 acatcttcga attttcgtcc gacatgtcta taacgtgttc agcactgatc gcgctgacaa
    76321 agacctgtga ttgcgtcgct tttaagcggc tggcaagcaa cccacgacgc tcatcatcaa
    76381 gctcagaggc aaaatcatct atcaggtaga gacaccgccg cccgctttca cgggtgagga
    76441 actctccttg cgccagacgt aaggcgcaca tcaacagctt aagctgccca cgcgataagg
    76501 tatcttccac cggcgcaccg tcggcgcgaa tgcgtaagtc cgctttatgc gggccatgcg
    76561 cggtgtaggt tagctggcga tcgcgttcaa aattacgttc cagcacctca gcatattctg
    76621 tctctttctc ccagccgcgc tggaaagaga aagtcagaga aaactcaggg agaaattgct
    76681 tacaggtatc ggccatatcg gccgcgatac cggcgctata ctccgcgcgc caggtgctga
    76741 tttgctccgc cagcgggatc agttctttat cccacgggcg tagctgttcg taacgtgtca
    76801 cctggcgcag cgccgcattg cgctgcttga gcaatcgctt gagattgctc caggcggtga
    76861 aaaatccggg ttcgttgtga aagcatcccc agtcgaggaa tgctcttctg tatttggggc
    76921 cgccgttgag taaagtaaac ccttctggcg ttatcagctg cattggcatc aggtgcgcca
    76981 gttccgcgac tttatgcccg tcagtaccgt cgatgcggac tttgctgtcg ccctgtttgt
    77041 ccttggttaa gccaatcgcc gtctcgcgct cttcgccctg taatcgccca tggagaacaa
    77101 atgcctcctg ctcatggcga atgacgcgac caatctgcaa actgcgaaac gcccgaccat
    77161 ggccgagcgt atagatggct tccagcacgc tggttttgcc actgccgttg gcacctacca
    77221 gaaagttaaa gccgggagat aaagcgagat ccgcggtttc aatgttgcgg aaatcgcgga
    77281 tcaacaagcg ggtgagggac attacagtct cattggcatg acaacatagg cagccgactg
    77341 tgatgcggca tcttcaatct gcacgctgga aaccgaatcg gtcagcatca tgcggacgtt
    77401 ttcgcatttc agcgcgttca gaacatccag cacatagctg acgttgaagc cgatttccat
    77461 ctccgcaccg ctataggtaa cgtcgaggat ctcttccgct tcttcctgtt ccgggttgtt
    77521 ggcggtgatt ttcagctggt tttcgctgac atacaggcgc acgccgcgga atttctcgtt
    77581 agagagaatt gccgcacggg caaacgcctg cttgagcaga tcgcagccag cttccagatg
    77641 tttgtccgga ttcttcggca gaacgcggcg gtaatccggg aagcgaccat ccaccagttt
    77701 ggaggtgaag ataaagtcgc caacgtgggc gcgaatattg ttgctgccaa tctgcacgcg
    77761 cagcggattg tcgccgccgt cgagcatacg catcagttca atcacgcctt tacgcggtac
    77821 gatcaccgaa tggcttggca aagattgacc aattggcatt gaacagaccg ccagacggtg
    77881 gccgtcggtt gccacggtgc gcagttcttc accttcggtt tcaaacagca taccatttaa
    77941 gtaatagcga acgtcctgat gcgccatcga aaactgggtc gcttcaatca gacgcttcat
    78001 cgttgcctgc ggcagggtaa attcgacttc actctgccag tcatcgaggt tcgggaaatc
    78061 cgccgctggc agggtagaca gcgaaaaacg gctacgcccg gagcgtacca gcatccgttc
    78121 accttccagc tgcacggcaa tttccgcgcc ttcaggcaga ccacggcaga tatcaaagaa
    78181 tttgcgcgcc ggaacggtcg tcgctcctgg ctcgtgtggc tgaaccagcg caacacgtgc
    78241 caccatttcc atctcgagat cagtaccggt cagcgacaac gtaccgtcag caacctgtaa
    78301 cagcagatta ccgagaatcg gtagcgtagg acgaccacct aacggaccgc tcacctgttg
    78361 tagcggtttt aataaatgct cacgttctac ggtaaatttc ataggtttac gatgacaatg
    78421 ttctgattaa atttgaaaaa tcttctttga tatcgtggct ctcttcacgc aactgctcga
    78481 tcttacggca ggcatgaagc accgtcgtgt ggtcacgacc accaaacgca tcgccaatct
    78541 ccggcagact gtggttagtc agctctttcg ccagcgccat cgccatctgg cgcggacgcg
    78601 ccaccgagcg ggatcgacgc ttggaaagga gatccgcgac tttgatcttg tagtactccg
    78661 ccaccgtctt ctgaatattg tcgatggtga ccagtttttc ctgcaatgcc agcaagtcgc
    78721 gcagcgcctc acgcacgaag tcgatggtga tcgcccgtcc ggtaaagttg gcattggcaa
    78781 tgacgcggtt cagcgcccct tccagctcac gtacgttaga tcgtagacgc ttggcgataa
    78841 agaacgccac ttcgcccggc aaacgaatgt cgttttcgtc ggcctttttc atcaggatcg
    78901 ccacacgggt ttccagctct ggcggttcga ttgccacagt cagtccccaa ccgaagcggg
    78961 atttcaaacg atcctcaacg ccgttgatct ctttcggata gcgatccgag gtgagaatga
    79021 tctgttgatt accttccagc agggcgttga aggtatggaa aaactcttcc tgagatcgtt
    79081 ctttattagc aaaaaactga atatcgtcga tcagcagtgc atctacggaa cggtagtagc
    79141 gtttaaactc ttcgatcgcg ttgttttgca gggctttaac catgtcctga acaaagcgct
    79201 cggagtgcat ataaaccact ttggcattcg gcttgcgcgc cataatgccg ttacccaccg
    79261 catgcagcag gtgagtttta cccagacccg tgccgccata aaggaacaac gggttatagg
    79321 caccgccagg gttatccgcc acctggcgag ccgccgcgcg cgccagttgg ttagatttac
    79381 cttcaacgaa gttatcaaac gtgtgtttga cgtttacgtt agaacgatag gtcggttctg
    79441 ccggggccgg gatgttatcc caacctgagc gcgtagaagg cgcagcacgt tgcggctgcg
    79501 tttgcgccac ctgtgcaggg gccgcgacgt tgctcgtcac tgccgcttgt ggcgtttgcg
    79561 tcaccggttt ggtgccgact tcaaaacgca gctgtggggc atccgctccg cagaaactgg
    79621 ttagcagtcc attgatatta ttaaggtact tgtcccgtac ccaatcgagg acaaaacggt
    79681 ttggcgcgta cagggccagc gtgttatcgc tcagttccgc ctgcaatggg cgtatccaca
    79741 tactgaattc tgtggctggt aactcatcct gcaatcgggc aagacactgc tgccaaagcg
    79801 aaagtgacac ggcggactcc actcgaacaa aagtcgataa tgactaaggc tgaaacattc
    79861 atgattgttg acgtacgtcg aaaagaccct gcttgagggt gacgcacgaa ccgctgtctg
    79921 cggttatatg cccgatcaag atcctgcaaa acgatcggga ccgcggatca tagcctaaac
    79981 tgcgcaagag atcttctgtt tctcacagat tttcccgatt tatccacagg actttccaga
    80041 cctcgctaag tgtaaacgat cctgccgcga ggcgggcacg atttacgccg catattggaa
    80101 aatttaatga ccatagacaa aaattggctt aatcgatcta ataaagatcc aggacgatcc
    80161 ttgcgcttta cccatcagcc cgtataatcc tccacccggc gcgccatgct ggttttcact
    80221 ggtgtgaggt cgtacatttt ccctgcgaaa aggtgcggaa aagcgcggta aataaggaaa
    80281 gagaattgac tccggagtgt acaattatta caatccggcc tctttaatca cccatggctt
    80341 cggtgtccat cgtttcattt ttcggcggat atccaataaa gccattgaat ttattcaagt
    80401 ttaggtagaa atcgccatga aacgcacttt tcaaccgtct gtactgaagc gcaaccgttc
    80461 tcacggcttc cgtgctcgta tggctactaa aaatggtcgt caggttctgg cacgtcgtcg
    80521 tgctaaaggc cgcgctcgtc tgaccgtttc taagtaataa agctaacccc tgagtggtta
    80581 agctcgcatt tcccagggag ttacgcttgt taactcccag tcaattcaca ttcgtcttcc
    80641 agcagccaca acgggctggc acgccgcaaa ttaccattct cggccgcctg aattcgctgg
    80701 ggcatccccg tatcggtctt acagtcgcca agaaaaacgt tcgacgcgcc catgaacgca
    80761 atcggattaa acgtctgacg cgtgaaagct tccgtctgcg ccaacatgaa ctcccggcta
    80821 tggatttcgt ggtggtggcg aaaaaagggg ttgccgacct cgataaccgt gctctctcgg
    80881 aagcgttgga aaaattatgg cgccgccact gtcgcctggc tcgcgggtcc tgatagccct
    80941 cattcgggtc tatcaacgcc tgattagtcc gctactcggg ccgcattgtc gtttcactcc
    81001 aacctgttca agctacggaa ttgaggcatt gcgcaggttt ggagtgataa aaggcagttg
    81061 gttgacggtg aaacgcgtat taaaatgcca ccctttacac cctggtggtg acgatcccgt
    81121 cccgcccgga ccatttgata ccagagaaca ctaacgatgg attcgcaacg caatctttta
    81181 gtcatcgctt tgctgttcgt gtctttcatg atctggcaag cctgggagca ggataaaaac
    81241 ccgcaacctc aggcccaaca gaccacgcag acaacgacca ccgcagcggg tagcgccgcc
    81301 gaccagggcg taccggccag tggccagggg aaactgatct cggttaagac cgacgtgctt
    81361 gatctgacca tcaacacccg tggtggtgat gttgagcaag ctctgctgcc tgcttacccg
    81421 aaagagctga actctaccca gccgttccag ctgctggaaa cttcaccgca gtttatttat
    81481 caggcacaga gcggtctgac cggtcgtgat ggcccggata acccggctaa cggcccgcgt
    81541 ccgctgtata acgttgaaaa agacgcttat gtgctggctg aaggtcaaaa cgaactgcag
    81601 gtgccgatga cgtataccga cgcggcaggc aacacgttta ccaaaacgtt tgtcctgaaa
    81661 cgtggtgatt acgctgtcaa cgtcaactac aacgtgcaga acgctggcga gaaaccgctg
    81721 gaaatctcca cctttggtca gttgaagcaa tccatcactc tgccaccgca tctcgatacc
    81781 ggaagcagca acttcgcact gcacaccttc cgtggcgcgg cgtactccac gcctgacgag
    81841 aagtatgaga aatacaagtt cgataccatt gccgataacg aaaacctgaa catctcttcg
    81901 aaaggtggtt gggtggcgat gctgcaacag tatttcgcga cggcgtggat cccgcataac
    81961 gacggtacca acaacttcta taccgctaat ctgggtaacg gcatcgccgc tatcggctat
    82021 aaatctcagc cggtactggt tcagcctggt cagactggcg cgatgaacag caccctgtgg
    82081 gttggcccgg aaatccagga caaaatggca gctgttgctc cgcacctgga tctgaccgtt
    82141 gattacggtt ggttgtggtt catctctcag ccgctgttca aactgctgaa atggatccat
    82201 agctttgtgg gtaactgggg cttctccatt atcatcatca cctttatcgt tcgtggcatc
    82261 atgtacccgc tgaccaaagc gcagtacacc tccatggcga agatgcgtat gctgcagccg
    82321 aagattcagg caatgcgtga gcgtctgggc gatgacaaac agcgtatcag ccaggaaatg
    82381 atggcgctgt acaaagctga gaaggttaac ccgctgggcg gctgcttccc gctgctgatc
    82441 cagatgccaa tcttcctggc gttgtactac atgctgatgg gttccgttga actgcgtcag
    82501 gcaccgtttg cactgtggat ccacgacctg tcggcacagg acccgtacta catcctgccg
    82561 atcctgatgg gcgtaacgat gttcttcatt cagaagatgt cgccgaccac agtgaccgac
    82621 ccgatgcagc agaagatcat gacctttatg ccggtcatct tcaccgtgtt cttcctgtgg
    82681 ttcccgtcag gtctggtgct gtactatatc gtcagcaacc tggtaaccat tattcagcag
    82741 cagctgattt accgtggtct ggaaaaacgt ggcctgcata gccgcgagaa gaaaaaatcc
    82801 tgattcggtg agttttcgct aaaataaggg cggtcagttg accgcctttt ttcttttcgt
    82861 agggcggata agcaccgcgc atccgccaca caaagcaaca ggaacatcat gagcgataat
    82921 gacactatcg tagcccaggc cacgcctccg ggacgtggcg gtgttggcat cctgcgcatc
    82981 tccggcctca aagcccgtga agttgccgaa accgtgctgg gtaaactacc taagccgcgc
    83041 tacgccgatt atttgccatt taaagacgcc gacggcagcg tgctcgatca ggggattgcg
    83101 ctatggttcc ctggcccgaa ctcgttcacc ggcgaagatg tgcttgaact gcagggtcat
    83161 ggcggtccgg tgatcctcga cctgctgtta aaacgcattc tgaccattcc cggcctgcgg
    83221 attgctcgcc ctggtgagtt ttccgaacgc gcgtttcttt acgataaact cgacttagcc
    83281 caggctgagg cgattgccga tcttatcgac gccagctcgg aacaggcggc ccgttcggca
    83341 cttaactcgc tgcaaggcgc attctccgca cgggttaatc atctggtaga agccctcacc
    83401 cacttgcgca tttacgtcga agcggcaatt gatttccctg atgaagagat cgatttcctc
    83461 tccgacggca aaattgaagc ccagctcaat gacgttattg ccgatcttga tgcagtgcgt
    83521 gctgaagcac gtcagggtag tttgttgcgc gaagggatga aagtggtgat tgccggacgt
    83581 cctaacgccg gtaaatcgag cctgttaaac gcgctggctg gccgtgaagc agcaatcgta
    83641 accgatatcg ccggaactac gcgtgacgtg ttgcgtgagc atatccacat tgacggaatg
    83701 ccgctgcata tcatcgacac cgccgggcta cgtgaagcca gtgacgaagt ggaacgtatt
    83761 gggatcgagc gcgcgtggca ggaaattgaa caggccgacc gggtgctgtt tatggtcgat
    83821 ggcaccacaa cagacgccgt ggatccggca gagatctggc cggaatttat cgcccgtctg
    83881 ccagcgaaac tgccgatcac cgtggtgcgc aataaagccg atatcaccgg cgaaacgctg
    83941 ggaatgagtg aagtgaacgg tcacgcgtta attcgtctct cggcaaggac tggtgaaggc
    84001 gtggacgtgc tgcgtaacca tctcaaacag agcatgggct ttgacaccaa catggaaggc
    84061 ggtttcctgg cgcgccgtcg ccacttacag gcgctggaac aggcagcgga gcatttgcag
    84121 caaggtaaag cgcaactgtt aggtgcctgg gcgggtgaac tgctggcgga agagttgcgc
    84181 ctggcacagc agaacttaag cgaaatcacc ggggaattta cttcagacga cctgctgggg
    84241 cggattttct ccagcttctg tattggtaag taaccgcgct tacgaagccg tattctgact
    84301 gtcagatgcg gcttcgcttc attgttagca ctcctgttat tcctcaaccc tttttttaaa
    84361 cattaaaatt cttacgtaat ttataatctt aaaaaaaagc atttaatatt gctccccgaa
    84421 cgattgtgat tcgattcaca tttaaacaat tttagaatag acaaaattct tgagtattat
    84481 actgtagcct cgtgtctttc gaggataagt gcattatgaa tatcttacat atatgtgtga
    84541 cctcaaaatg gttcaatatc gacaataaga ttgtcgatca ccgcccttaa tttacccttc
    84601 tgtagccatc accagagcca aaccgattag attcaatgtg aacgattagt ttgctatatc
    84661 ttaattttgc cttttgcaaa ggccatctct cgtttattta cttattttag taaatgatgg
    84721 tgcttgtata tatacatggc gaattaatgg atattgcaga tgtaatattc acagggatca
    84781 ctgtaattaa aataaatgaa ggattatgta atggaaaact ttaaacatct ccctgaaccg
    84841 ttccgcattc gtgttattga gccagtaaaa cgtaccactc gcgcttatcg tgaagaggca
    84901 attattaaat ccggtatgaa cccattcctg ctggatagcg aagatgtgtt tatcgattta
    84961 ctgaccgaca gcggcaccgg ggcggtgacg cagagtatgc aggccgcgat gatgcgcggc
    85021 gacgaagcct acagcggcag ccgcagctac tatgcgttag ccgagtcagt gaaaaatatc
    85081 tttggttatc aatacaccat tccgactcac cagggccgtg gcgcagagca aatttatatt
    85141 ccggtactga ttaaaaaacg cgagcaggaa aaaggcctgg atcgcagcaa aatggtggca
    85201 ttctctaact atttctttga taccacgcag ggccatagcc agattaacgg ctgtaccgtg
    85261 cgtaacgtct atatcaaaga agccttcgat actggcgtgc gttacgactt taaaggtaac
    85321 tttgaccttg agggattaga acgcggtatt gaagaagttg gcccgaataa cgtgccgtat
    85381 atcgttgcaa ccatcaccag taactctgca ggtggtcagc cggtttcact ggcaaactta
    85441 aaagcgatgt acagcatcgc gaagaaatac gatattccgg tggtaatgga ctccgcacgc
    85501 tttgctgaaa acgcctattt catcaagcag cgtgaagcag aatacaaaga ctggaccatc
    85561 gagcagatca cccgcgaaac ctacaaatat gccgatatgc tggcgatgtc cgccaagaaa
    85621 gatgcgatgg taccgatggg cggcttgctg tgcatgaaag acgacagctt ctttgatgtg
    85681 tacaccgagt gcagaaccct ttgcgtggtg caggaagggt tcccgacata tggcggcctg
    85741 gaaggcggcg cgatggagcg tctggcggta ggtctgtatg acggcatgag tctcgactgg
    85801 ctggcttatc gtatcgcgca ggtacagtat ctggtcgatg gtctggaaga gattggcgtt
    85861 gtctgccagc aggcgggcgg tcacgcggca ttcgttgatg ccggtaaact gctgccgcat
    85921 atcccggcag accagttccc ggcacaggcg ctggcgtgcg agctgtataa agtcgccggt
    85981 atccgtgcgg tagaaattgg ctctttcctg ttaggccgcg atccgaaaac cggtaaacaa
    86041 ctgccatgcc cggctgaact gctgcgttta accattccgc gcgcaacata tactcaaaca
    86101 catatggact tcattattga agcctttaaa catgtgaaag agaacgcggc gaatattaaa
    86161 ggattaacct ttacctacga accaaaagta ttgcgtcact tcaccgcaaa actgaaagaa
    86221 gtttaattaa tactacagag tggctataag gatgttagcc actctcttac cctacatcct
    86281 caataacaaa aatagccttc ctctaaaggt ggcatcatga ctgatcaagc tgaaaaaaag
    86341 cactctgcat tttggggtgt tatggttata gcaggtacag taattggtgg aggtatgttt
    86401 gctttacctg ttgatcttgc cggtgcctgg tttttctggg gtgcctttat ccttatcatt
    86461 gcctggtttt caatgcttca ttccgggtta ttgttattag aagcaaattt aaattatcct
    86521 gtcggctcca gttttaacac catcaccaaa gatttaatcg gtaacacctg gaacattatc
    86581 agcggtatta ccgttgcctt cgttctctat atcctcactt atgcctatat ctctgctaat
    86641 ggtgcgatca ttagtgaaac gatatcaatg aatttgggct atcacgctaa tccacgtatt
    86701 gtcgggatct gcacagccat tttcgttgcc agcgtattgt ggataagttc gttagccgcc
    86761 agtcgcatta cctcattgtt cctcgggctg aagattatct cctttgtgat cgtgtttggt
    86821 tctttcttct tccaggtcga ttactccatc ctgcgcgatg ccaccagcac cactgcggga
    86881 acgtcttact tcccgtatat ctttatggct ttgccggtgt gtctggcgtc atttggtttc
    86941 cacggcaata ttcccagcct gattatttgc tatggaaaac gcaaagataa gttaatcaaa
    87001 agcgtggtat ttggttcgct gctggcgctg gtgatttatc tcttctggct ctattgcacg
    87061 atggggaata ttccgcgcga aagctttaag gcgataatct cctcaggcgg caacgttgat
    87121 tcgctggtga aatcgttcct cggcaccaaa cagcacggca ttatcgagtt ttgcctgctg
    87181 gtgttctcta acttagctgt tgccagttcg ttctttggtg tcacgctggg gttgttcgat
    87241 tatctggcgg acctgtttaa gattgataac tcccacggcg ggcgtttcaa aaccgtgctg
    87301 ttaaccttcc tgccacctgc gttgttgtat ctgatcttcc cgaacggctt tatttacggg
    87361 atcggcggtg ccgggctgtg cgccaccatc tgggcggtca ttattcccgc agtgcttgca
    87421 atcaaagctc gcaagaagtt tcccaatcag atgttcacgg tctggggcgg caatcttatt
    87481 ccggcgattg tcattctctt tggtataacc gtgattttgt gctggttcgg caacgtcttt
    87541 aacgtgttac ctaaatttgg ctaaatcctt caagaagcca gccattcgct ggcttcttgc
    87601 ctctcaggaa atcacttatg tccaaatggc aactcgcctg attctccttc accacgtatg
    87661 ctttgcgtca ccttactatc aggacgcttt agcccatgtc ccgcttttta atttgtagtt
    87721 ttgccctggt tttactttat cccgccggga ttgatatgta cctcgttggt ttaccgcgca
    87781 tcgccgccga tctcaatgcc agcgaagcgc agttgcatat tgcgttctcc gtttatctgg
    87841 cgggcatggc agctgcgatg ttatttgccg gtaaagtggc cgatcgttca gggagaaagc
    87901 ctgtcgccat ccccggcgcg gcgctattta ttatcgcttc ggtgttctgt tcactggctg
    87961 aaaccagcac gttatttctt gcagggcgct ttctacaggg gttgggcgca ggctgttgtt
    88021 acgtggtggc gttcgccatt ttgcgcgaca cgctggatga tcgacgtcgg gctaaagtgc
    88081 tgtcgttact caacggtatt acctgcatca ttccggtgtt agcgccggtg ctcggacacc
    88141 tgattatgct taaattccct tggcagagtc tgttctggac gatggcaatg atgggcatcg
    88201 cggtactgat gttatctttg tttattttaa aagaaacgcg cccagcgtcc cccgctgctt
    88261 cggacaaacc acgagaaaat agcgagtcgc tgcttaatcg gtttttcctc agccgtgttg
    88321 ttatcaccac cctcagcgtt tcggtgatcc tcactttcgt caatacatcg ccggtattgc
    88381 tgatggaaat catgggtttt gagcgcggag aatacgccac cattatggcg ttgaccgctg
    88441 gcgtcagcat gaccgtttca ttctccacgc catttgcgct gggaattttt aagccacgta
    88501 cgttgatgat cacctcgcag gtgttattcc ttgcggcggg gatcaccctt gccgtttcac
    88561 cttcccatgc ggtttctctg tttggtatca cgctgatttg cgccggtttc tcggtaggtt
    88621 ttggcgtagc gatgagtcag gcgttaggac cattttcatt acgcgcgggc gtagccagct
    88681 cgaccttagg tattgcgcag gtttgcggtt cgtcactgtg gatttggctg gcagcggtgg
    88741 ttggtatcgg cgcatggaat atgctgatcg ggattctgat tgcctgtagc atagtgagcc
    88801 tgttactgat tatgttcgtc gcgcctggac gccccgttgc cgctcatgaa gaaatccatc
    88861 accacgcttg atctcaatct gctgctctgt ctgcaactgc tgatgcagga gcgcagcgtg
    88921 accaaagcgg cgaagcggat gaacgtgacc ccttcagcgg tgagtaagtc gctggcaaag
    88981 ttaagagcgt ggtttgacga cccgctcttt gtgaactcac cgctggggct gtcgcccaca
    89041 ccgctaatgg tcagcatgga gcaaaatctg gcggaatgga tgcaaatgag caatctgctg
    89101 ctggataaac cgcaccacca gacaccgcgc ggcctgaagt ttgagttggc ggcggaatcg
    89161 ccgctgatga tgatcatgct taatgcgctg tcgaaacgga tctaccaacg ttacccgcag
    89221 gcgaccatca aactacgtaa ctgggattac gattccttag atgccattac tcgtggtgaa
    89281 gtggatattg gtttttccgg tcgcgaaagc catcctcgct cgcgggagct gttaagctcg
    89341 ctaccgttag ccattgatta tgaagtgctg tttagtgatg taccctgcgt ctggttacgc
    89401 aaagatcatc cggcactgca tgaaacgtgg aatctggaca cctttttacg ttatccgcat
    89461 atcagcattt gctgggaaca gagcgacacc tgggcgctgg acaatgtgtt acaggagctg
    89521 gggcgcgaac gcacgattgc tatgagcctg ccggaatttg agcagtcact gtttatggca
    89581 gcgcaacccg acaatctgct actggcgacc gcgccgcgct actgtcagta ctacaatcaa
    89641 ctccatcaac tgccgttggt tgctcttcct ctcccgtttg acgaaagcca gcaaaaaaag
    89701 ctggaagttc cttttaccct gctgtggcat aaacggaaca gccataatcc gaagatcgtc
    89761 tggttacggg aaaccattaa aaacctttac gcgtcgatgg cataaccgaa tcgtatgaaa
    89821 cgcgacccaa tttcacaata aaatgtaaaa aagttgtaat aagcttgtct gaatcgaact
    89881 tttagccgct ttagtctgtc catcattcca gtaaatgatt actcttatat tcataatgga
    89941 ccattaagca tggagcgaaa aatggcgact cactttgccc gagggatttt aacggaagga
    90001 catctgattt ctgttcgtct cccctcccag tgtcatcaag aagcccgaaa cattccccct
    90061 catcgtcaaa gccgttttct ggcgtccaga ggtttactcg cagaactgat gttcatgctg
    90121 tatggcattg gcgaattgcc ggaaatcgtc accctgccga aaggtaaacc ggttttcagt
    90181 gataaaaatt tgccttcgtt ttctatttcc tatgccggga atatggttgg cgtggcgtta
    90241 acaaccgaag gtgaatgtgg cctcgatatg gaactacagc gtgcgacgcg cgggtttcat
    90301 agcccacacg cgcccgataa ccacaccttt tccagcaatg aatcgctatg gatcagtaaa
    90361 caaaacgatc ctaacgaagc gcgggcgcag ctcatcacgc tgcgccgaag cgtgctaaaa
    90421 ctaaccggtg atgttttgaa tgacgatccg cgcgatctgc agctgctgcc cattgccgga
    90481 cgcctgaaat gtgctcatgt aaatcatgta gaagcgttat gcgacgcgga ggacgtgcta
    90541 gtgtggtccg tggcggtcac gcccgcgatt gaaaagctca gtgtctggga gttagatggc
    90601 aaacacagct ggaaaagcct gccggatatt cacagccgcg ccaacaatcc taccagccgg
    90661 atgatgcgtt ttgcccagct ctctaccgtg aagtcttttt cgccaaattg atagacaacc
    90721 acaggagtca ttatgtctga aaaattgcag gtggttacgt tactggggag cctgcgcaaa
    90781 ggctcattta atggcatggt tgcacgtacc ctgccgaaaa ttgctccggc gagcatggaa
    90841 gtcaatgcgt taccttccat tgccgacatt cccctgtatg acgctgacgt acagcaggaa
    90901 gaaggttttc cagcaacggt tgaagctctg gcggaacaga tccgtcaggc tgacggtgtg
    90961 gtgatcgtca cgccggaata taactactcg gtaccgggtg ggctgaaaaa tgccatcgac
    91021 tggctttccc gcctgccgga tcaaccgctg gccggtaaac cggtgttgat tcagaccagc
    91081 tcaatgggcg tgattggcgg cgcgcgctgt cagtatcacc tgcgccagat tctggttttc
    91141 ctcgatgcaa tggtgatgaa caagccggaa tttatgggcg gcgtgattca gaacaaagtt
    91201 gatccgcaaa ccggagaagt gattgatcag ggtacgctgg accacctgac cgggcaattg
    91261 accgcatttg gtgagtttat tcagcgagtt aagatctaaa taaaaaaccc gccagcaatc
    91321 atgcatggcg ggtttttaac gcgctatcga ttttagtgag cgtcgataaa gacaatcttc
    91381 aggataaaca gcagcgcaac gatgattacg cacgggctta gatcacgcag acgtccggta
    91441 ccgattttca tcacgcagta ggagataaag cccagcgcga taccttcggt aatcgagaag
    91501 ctgaacggca tcatcacggc ggtaataaac gccggaacag attcagtaag atcctgccag
    91561 ttcacgcgtg ccagactgga agtcatcagc acgccaacgt aaatcagcgc accagctgca
    91621 gcgtagcctg gcaccatccc cgccagcggc gacagaaaga taaccagcag gaacagcaga
    91681 ccaacaacca ctgccgtcag accggtacga ccgccaaccg atacgccgga agaggactca
    91741 atataagccg taacggaaga agtaccgata aacgaaccgg tcacggaaga gatactgtcg
    91801 acatacagcg cctgcttcat gcgcgggaat ttacccttct catccgccag gcctgcttta
    91861 tcggtcacgc caatcagcgt accggaggag tcaaacaggt tgaccagcat gaaagagaaa
    91921 atcacccctg ccagcccgag gttaaacgac ccggctaaat ctacatgacc cacaactgtc
    91981 attacgctcg gcggcgcaga aacgatgcca ttgtagtgca catcacccag catccagccc
    92041 agcagcgtcg tcaccacgat agaaaccagc accgctgcgt gaatgttgcg cgaggccaga
    92101 atagcaatga tgaagaagcc gaggataccc agaagtacgc tgtgagaagt cagattaccg
    92161 atgctcacca gcgtttccgg gttagcgaca atcacacctg cgtttttcag ccccatcatg
    92221 ccaatgaaca gaccgatacc gctggtaata cccacacgca gactcaccgg aatgttggca
    92281 atcatccagt agcgaacgcg gaaaatcgtc agtaacagca gacctatcgc gccccagaag
    92341 attgcgccca tcccgacctg ccacggcaag cccatcgcct gtacaacgac aaaagcgaag
    92401 aacgcattca ggcccatagc gggtgccagt gcaactggca ggttagcaaa cagtcccatc
    92461 ataatactgc cgaatgcagc gatcagacag gtagtgacga agacggcgct ggtatccatg
    92521 ccagcaacgc caagaatttg cgggttaaca aaaacgatgt aaaccatcgt caggaaggtg
    92581 gtaaaaccgg cgatcacttc ggtccgtgcc gtcgtgccat gttcgcgcag tttaaacacg
    92641 cgttccagca tcccctggcc agaagcctgg gttgtgtgtt gttgactcat tatctatttc
    92701 cgaacaagga gggaaaatcc gtcgctatcg tataccaaaa tgagacaata tggacggctg
    92761 aagataactt tttttcattg tatttgctta tacggcaacg attgcgtcat gcgaattggc
    92821 atgacgaaaa cgttaaacta acgataaagg aaagacatgt cccagataga agcggtattt
    92881 ttcgactgcg acggtacgct ggtcgacagt gaagtcattt gctcccgcgc atatgtaacg
    92941 atgtttcagg aatttggtat tacgctcgat cctgaagagg tattcaaacg tttcaaaggt
    93001 gtaaaactgt acgaaattat cgatattgtt tcccttgaac atggtgttac gttagcgaaa
    93061 acagaagctg aacacgttta ccgtgcagaa gtcgctcggc tgttcgattc agaactggaa
    93121 gccatcgaag gggctggagc gctcctgtca gcgatcactg cgccaatgtg tgtggtatct
    93181 aacggcccaa ataacaaaat gcagcattct atgggcaagc tgaatatgtt gcactacttc
    93241 ccggataaac tgttcagcgg ctacgatatt cagcgctgga agccagaccc ggcgttaatg
    93301 ttccatgcgg caaaagcgat gaatgtaaat gtagaaaact gcattctggt tgatgactca
    93361 gttgccggtg cgcaatctgg tatcgacgca ggtatggaag tgttctactt ctgcgccgac
    93421 ccgcacaata agccgatcgt tcacccgaaa gtcaccacct ttacccatct ttcgcagtta
    93481 ccggaactgt ggaaagcgcg tggttgggat attacggcat agttcttcac actcccttca
    93541 cttaccccgc ttaaattggc gctcaaaggt aagtaaaggg agtttgatat gtctgtttca
    93601 cgtcgggtaa tacatcacgg actttatttt gcggttttag gaccgttaat tggtgttctg
    93661 tttcttgtcc tctacatatt cttcgcaaaa gaaccgctgg ttcttttgcg aagaatacag
    93721 gtacttccat tgtttctctt attgtcgatt actacgggag ttattcctgc attgttaacc
    93781 ggcgtgatgg tcgcctgtct gccggaaaag atcgggtcac agaaaaatta tcgttgtctg
    93841 gctggtggca taggtggcgt cgttatcacc gagatctatt gtgcagttat tgtacatatt
    93901 aagggcatgg cttcctcgga gttgtatgaa aacattcttt ctggtgacag tctcgttgtc
    93961 cgcatcattc ctgcattgct ggcaggtgtg gtgatgagca gaatcattac ccgtctgccc
    94021 ggattggata tttcatgtcc tgaaacagac tctttaagtt aagcgggata ctttatcttt
    94081 gggctactca aaagcagaca ggatgttact atgactcaaa atatcaggcc gttaccccaa
    94141 ttcaaatatc atcccaagcc actggaaaca ggcgcatttg aacaggataa aaccgtagag
    94201 tgcgattgct gtgaacaaca gacgtcagtt tattactcgg gtccctttta ttgcgttgat
    94261 gaagttgaac atctctgtcc gtggtgtatt gcggacggtt ctgctgctga aaagtttgca
    94321 ggtagttttc aggatgatgc cagcatagaa ggtgttgaat ttgagtatga tgaagaggac
    94381 gaatttgccg gtattaagaa cacatatcct gatgaaatgc tgaaagagtt ggttgaacgc
    94441 acgccaggtt atcatggatg gcaacaggaa ttctggcttg cgcattgtgg cgatttctgt
    94501 gcttttatcg gctatgtggg ctggaatgat ataaaagatc gcctcgatga atttgccaac
    94561 cttgaagaag attgtgagaa tttcggtatt agaaattctg atctagctaa atgcctgcaa
    94621 aagggtggtg attgtcaggg ttatctcttc cgctgtctcc actgcggcaa gctgagactg
    94681 tggggtgatt tttcgtagtt atttaaataa tgagaacagg ccggagcgta attcatacat
    94741 ccggccttat ttcttcagct taatcgagcg ctaattctgc ggcagcggct ttatcagcga
    94801 ttaccatcag cgaaggatgc agctgcaaca cggaagctgg aacatcttcg gtaaccggac
    94861 cttgcagcac atttttcagt gcctgcgctt tacctgcgcc actgacaatt atcagcaagt
    94921 ttttcgccgc catgatgctt ttcggcccca tcgtcacgta gctgtcaggc actaacgaaa
    94981 agtcgccgcc caactcacca tgcgccacaa tatcaaccat ctctccctgg atcgggaact
    95041 ccacggtttg ctcatggaaa tgggtcgtat tcggcaaatt gccacaaaaa tgaccatctg
    95101 cacctaatcc cagcaccacc aaatccaggc cgccttcacg cgccagtttc tgatcatgct
    95161 cgcggtagtt atcaatagtg agcttctgga tattctcttc tttgatcccc gcaggggtga
    95221 aaaacagatt acgcagattg gtaatcgtta caccttctcc ctctttgccg cgaaagggaa
    95281 tttcatcgaa attatagaaa tagcagttat cataccaggg cttaccttta accagggtgg
    95341 tgaggtattc atacatgcct ttgggcgtgc taccggcggt aattgccagg ttaacacgac
    95401 gcgtcttcga catataacca agcagatggt gtgccgcgac acggctcatt tcctggtaat
    95461 cttcggtaat gattaatttc atcttagtgc cttattttat ttaaataatt tctgtacgaa
    95521 ggctgcatag gcaggccgcc agacatccat ttcatggttc aggccgggat attcctggta
    95581 atcaaactta atttttttct gctcaagctc agttttcagc ccggcgatat ccttgccggt
    95641 tacgacatct ttatcaccaa cgaccacagt aaaattacgt agttgctggt taatagctgc
    95701 cggatcgttc agccgggccg cgacaccttc atccggtacg gttgtcgtgg taacaccact
    95761 gaatgtggcc agccagccaa agctttccag atgattcatt ccggaaacca gcgcctggta
    95821 cccgccttgt gaaagccctg ccagcgcgcg accatcggca tctttacgga cattaaaacg
    95881 cttgctaatc agcgggataa tatcgttcat cagttcgcga tctgccgctt tagcattcag
    95941 cggataaaag actttacgtc tttcctgagg cacgaaatct tcgggaataa tgcccttcgc
    96001 atcggtttcg gtatccggga tcaccaccag catcggttta attttccctt cagccagcag
    96061 gttatccatg atttgcggga tacgcccctg atcgatagcg gaacgtccgg tatcaccaaa
    96121 accgtgatag aaatagagca ctggcaaagg ctcgcccatg ccggtgtaac ctggcggggt
    96181 ccagacatac atctgacgtt cagattgcaa tgcgttggag tggtaagtta tggcgatcaa
    96241 atcaccatgc gcaacagaac gcgtatccag ataactgcct ggcaccagaa tcatactgga
    96301 gttaacctgg cgctgaggct tagtcattgc ggtgcctgta tcaatgctgc gtacaccatc
    96361 aacattgaaa aaatactcgt acaggttgcc tttcaggata ggtgtgcgcc acgaccagac
    96421 tcctgcttcg tctttggtca tcgggtgaat attgtccgga accggaacac ccaccactac
    96481 agagacattt tttgccccag gggcaaagta gcgaaaggtc acgctgttat cggcattgac
    96541 ctgagtcaca tactgtttca ccgggatagt gggtgccggg ctggctggca tatccgctgc
    96601 tatggcccac tgagcagaaa taccgctggc aatagccagc gttaaagcag caatttttat
    96661 attcatcgta atattccttt cattaagttc cattaccacc agatttcagc ctgggcaccg
    96721 acagcgaact ggtcgtcttt attgtcttcg aaggtgaagc catccagttc gttttccagg
    96781 gctttgatat acgtggcgta gaaacgaatt tccggacgcg aggtcaacat actggtattg
    96841 actttaaagg tatggaagag cgtggtttta taaccagact cattaaattt attactattc
    96901 gcatctttgt tttgctgggt gaaatagccc agttcaacac ctgtctggtt atattggtcc
    96961 caaatatagg ccgggcgaac aaccgcacga atagattcga aatcagagtg tgcgcctgtt
    97021 tcgtagctat aaatatcgtt accgaaggag taaacaatcg cgttagccac aataaaatga
    97081 tcgccgatat aggcttcgcc ctgtgaagtc agacgaactg ctgttccgcc ggtgtgatca
    97141 ccataataac gaccattaaa ggtggtaaat ggacttgcgc cagcataacg gccaaagtta
    97201 ctggcgatag agttattcgc aaccaggaag gagaattcgt tgaagccacc tttatcaaat
    97261 ttctgcgtta aagatgtgcc aaacatccag gtatctttcc agtcataata gccgttatta
    97321 tcctgattat ctttttccga tgcgctttcg tttgccgtga cataacgacc actcaccatt
    97381 aaggtcgctt tatcccataa cgggatatct ttatagcgta aatcaatagt attggtatta
    97441 atctgctgtt tgttttgcag gctgcgatcg taatcatcga catcttcgcg aaccagcgcg
    97501 atatcaattt tacccggacc gactttccag ttttccagac ctacacccgc tgcggcatca
    97561 gtacgctgcg ttttccagtc cagcatctgg atttcaattt tcggcgcacc gtgtttaccc
    97621 acccagaaat cagcctctgg cgcaaagggc aggaaacctt tggtggtaac gtacatatcg
    97681 gagaactgca taaagttctc gccaccggcg ttatcgccaa accagccggt agagtactgc
    97741 tgaccaacgt taccatccat catcacaacg gcatcaaccc gtttgccgtt ttcgttgtag
    97801 acacgttgtt ttaactgcaa atcaaaccag ccggagtatt cgttaccaaa gcggcccaga
    97861 gaaccaatcg cccatgattt aggtgaacca tgagaggcgg tcccccaacc agaacggtaa
    97921 tagccgttgt aactaaagcc aatttcatct ttcacaaact tactgaaatc tttcagagtc
    97981 atggaagaat aaatcgagcc tgtctcactg gcatttttct cttcattttt gaccagtaca
    98041 gcatcaggtt ttgcggcact actggtcggg aacggattgg cggcataccc ttgatcattc
    98101 gtacttacgc tacgattcac cgtcgctggc gtatattttt tcttctcttc atctttatac
    98161 tttttgagtt cgctttgcgt ttctcttaac gctttttcta ataactcaag gcgatgttcc
    98221 accgttaatg attcagccaa tgattgtgca gaaaaggcta acggtgccat taataagatg
    98281 gcagaggtaa taagatttcg tctaaacata atatcccttt atggtgcaaa gaaagaatta
    98341 acgtatcgca tccagactgt tctgaatgcg acgataatta aggtgcttta ttggttattt
    98401 ttttaatgac agcccccgcg ttttgatcac ttctgcatac catccgaagc ttttcttacg
    98461 cgttcttgtg aggcttcctt cgccattatc atcacgatcc acataaataa agccgtagcg
    98521 cttagacatt tgtgaatgag atgcactgac taaatcaatt ggcccccaac tggtgtaccc
    98581 cataatatcc acaccatcgg caatcgcttc atttacctgt accaggtgat cgtttaaata
    98641 ggcaattcga taatcgtcct gtatcgaacc atccgcttca acgctgtctt ttgcgcctaa
    98701 tccgttctcg acaataaata acggtttttg ataacgatcc caaagcgtat ttaacagaac
    98761 ccgtaatcca accgaatcaa tttgccaccc ccactctgaa cttttcagat gcggattggg
    98821 gatcatattc agtatgttgc cctgcgcatt tttattaatg ctttcgtcgt gggaaacaca
    98881 accagtcatg taataactaa aagagatgaa atcgacggta tgttttaaat cttctgcgtc
    98941 actttcagtc atctcaatgg tgatattgtg gtcgcggaag aaacgctgca tatagccggg
    99001 atactggcca cgcgcctgaa catcaccaaa gaacatccag cgccggttct cttccatggc
    99061 ctgcaacata tcctgtggct ggcaggtgag ggggtaaacc agcccaccga gaagcatatt
    99121 gccgattttc gcttcgggga gcaggctatg acaggcttta actgcccgcg cactggcaac
    99181 cagttgatgg tggatagcct gataaacttc cgcctcgcca ctctcttctg ccagccccac
    99241 gcccgtgaat ggctcgtgta acgacatgtt gatttcatta aacgtcagcc ataacgccac
    99301 tttatgttgg tagcgagtaa agaccgtgcg ggcgtaatgc tcgaagtgat cgatgaccgc
    99361 tcgattagcc caaccgccgt agtttttcac cagcccatat ggcatttcgt aatgggataa
    99421 cgttaccagc ggcttgatcc ccgcctgcgc catttcatca aacagccgat cgtaaaacgc
    99481 taaccccgct tcattcggtt cgacttcgtc gccctgaggg aaaattcgcg cccaggcaat
    99541 ggaaatacgc agacaggtga agcccatctc ggcaaataac gcgatatctt ccgggtaacg
    99601 gtgataaaaa tcgatggcga catctttgat attctctttc cccaggatgc gcggttccat
    99661 ttttcccatt acgccatgag gctgtaaatc tgaggtcgag atccctttgc catcttcctg
    99721 ccaggcacct tccacctgat tggcagctgt tgcgccaccc caaagaaatg tttctggaaa
    99781 tgctttcata attaactcct tttatcgtta gcgaatgatg gataacagcg gttcacctgc
    99841 gcttatctgc gccgtgccgt ggggtaatac gtccgtaaaa tcatcgctat tactgattaa
    99901 taccggcgtc gtcagatcaa atccggcctc gcgaatagca gggatatcaa aagaaatcag
    99961 ccgatcgcct gtattgacct tgtcacccac gttgacgtga gcggaaaaga atttgccgtc
   100021 cagttttacg gtgtcgatac cgacatgaat caggagctcc acaccatcat ctgactcaat
   100081 gccaatggcg tgtaatgtgg cgaacaacga agcaattcga cccgcaaccg gagaacgcac
   100141 ttcaccaacc gagggcagaa tggcgatacc tttacccaac aggccactgg caaacgtggt
   100201 atcagcgacg tgaatgagcg gcacaatctc tcccgtcatc ggtgaacaga taccgccctg
   100261 ctcaggtggt gtaataacct ctggtgtttt ctcttgcggg gcaccctgcg ctggctgacg
   100321 tttagcggtg atgaaatgaa gcatcaccgt accgacaaat gcgcaaccga tggcaatgac
   100381 accgccaata acgctggccc agacggtgaa atcaattccc gttgacggga tggtttgcat
   100441 gaaggtgaaa atacttggca aaccaaagga gtacactttc gtttgcgcgt agccaatgat
   100501 ggtggccccc aaagccccac tgatacaggc gataacaaag gggtacttac gcggcagatt
   100561 aacgccatat accgctggtt cggtgatacc aaacaggccc gtcaacgccg ctgatcccgc
   100621 caccactttt ttctgcgcat cgcgttcgca gaggaagacg ccgagcgccg ccccgacctg
   100681 cgccataatg gcgggcatta acagcgggat catggtgtcg tagcccagca cggtgaagtt
   100741 attgatacac agcggcacca ggccccagtg cagtccgaac atgacgaaga tttgccagaa
   100801 gccgcccatt accgcgcctg caaatgcagg aaccgcctga taaagccaga gataaccggc
   100861 ggcaatcagt tcgcttatcc aggttgatag cggccccacc agcagaaagg tgacgggtgt
   100921 gataaccatc agacatagca atggtgtgaa gaaatttttg attgccgacg gtaaccacgc
   100981 attaagtcgg cgttccagaa tgctgcacaa ccaggcagaa aaaataatgg gaataaccga
   101041 tgacgagtaa ttcaacaatg tgaccggaat acccaggaaa tccagcccca gctcatccgc
   101101 tttttgcccg ttctcgaaag cagtcagaat taatggatgc actaacgctc caccaatcac
   101161 catggcagta aatggattac cgccgaagcg tttccccgcg gtgtatccca ggattatcgg
   101221 gaagaaccaa aacaaggcat cactggcgct gaataaaatt aaataagtac cactttgttc
   101281 ggtcgtccac tgaaaagtga gcgccagagc cagcatacct ttcaggatcc cggttgccgc
   101341 catcaaaccg atcagaggcg taaaaatacc tgaaataaca taaacaaagc ggtttagcag
   101401 attacctttc tcatcatttt ccggtgcctg ttgcgctttt tcgccaaggc ctgccacgct
   101461 gttgaccgcc aggaagacat cggccacatg gttacctatg accacctgaa actgaccacc
   101521 gctttccacc accataataa taccgggggt ctttttcagt acctcagctt gcgctttgct
   101581 ttcatccttt aacttaaaac gtaatcgcgt tgcgcaatgc atcagactca caatgttatc
   101641 tgcgcccccg actcctgcga ctatttttct ggctaactcc gtcataactt gccctctacc
   101701 gctttgcggc aaaactccaa aaaaaaacct gaaaaaaacg gcctgacgtg aatcaagcaa
   101761 tttttttcag gttttgcccg cttagtgcgg taacaatcct ttactcagta ataatatttc
   101821 agtgttcttt gcgcacgcgc tctatattta tggctaaaaa cataatctct gcggatgaaa
   101881 ttttacgttg atactgcaaa ccaataaaaa tggcgatccg ttccgcacat tgccatgctt
   101941 gcgggtaatt ttgttttact gcttgttgta atgattcatc actatcgtta attgaagcat
   102001 gttcaagaat acgccaggat aaaaacttca gatgtgtaac cagtcgctga taactcaagc
   102061 tttcttcctg gtaattaagg ctgaactgaa attttattaa ttgcagcatt tcgcgcatta
   102121 actgcgtgac acctgcaaca tcctccatat ttccgctcat ttgggcactg accagatgca
   102181 tggcaataaa gcccacttca tctttcggta actgcacgcc caaccgttta tcaataatgg
   102241 ttaacgcttc ttcccctagc tggaactctt tcgggtaaag ccgctggata tcccacagca
   102301 acgggttggg cagcaacacg ttttgctgaa agcgtttaat cgcaaactgg caatggtcag
   102361 ttagcgagat ataaatactg tcctgtaatt ttcccaagcg ctcctgcgct aaagagataa
   102421 tacgatcaca ggttgccatc acctcaagag gaatatgact taagagttcg cttaatcgcc
   102481 cgttcagttc atgactgctc aaggcatact ctttttctat tccacttgag ttaattcttt
   102541 cgccagcgcg tttttgaaag ccaattcctc gccccatgac gactttttcc cgctgttgat
   102601 catcaataac caccacaaca ttattgttga gaattttggt gatttgcatg ttcatagcaa
   102661 ggaccttttt tataaacaaa aaaacccgac ttcaccagta ttctctggtt atgtcaggtt
   102721 ttgcctgcga atgcagtaac aatccagtca tttattaatg gtttttataa cgaacatcca
   102781 ggttcggaaa ttaatttaat tgcgtgcaga gaaaatagca atgcgctatt gataaaaata
   102841 tgaccatgct cgcagttatt aactttgtgt aattttagga atttataaag ttatatataa
   102901 caaatcccaa taattaagtt attgggattt gtctggtgaa ttatttgtcg ctatcttttc
   102961 ccgccagcag tttatccagc tcatcaccgc ctacatgacg gaaatcctgc cccttcacgt
   103021 agtagaagat aaactcacaa atattctggc agcggtcgcc aatacgttcg atagaacgcg
   103081 cgcagaacag cgcagtaagt acgctcggaa tggtacgcga atcttccatc atgtaggtca
   103141 tcagttgacg aacaatacct tcgtattcct gatcgacttt tttatcttca cgataaatac
   103201 gtaccgcttc gtcaatgtcc atccgcgcga acgcgtccag cacgtcgtgc agcatctgga
   103261 tggtatgacg gcccagcgac tccagactta ccagcaacgg ctgatgctgc tgggagaatt
   103321 tctccagcgc agtacggcag attttgtccg ccacgtcgcc aatacgctcc agctcggcaa
   103381 tggttttact gatcaccata accagtcgca ggtcgctcgc cgtcggctga cgtttggcga
   103441 taatgcgcac gcaggcttca tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt
   103501 cgatcacgcg cttcgccaga tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa
   103561 gctgctgctc caccatgccg cccatggtca tcacctgcgt gcggatactt tccagttcgg
   103621 cgttgaactg gccggaaata tgtttattaa gattgagact gtccataacg cactcctgaa
   103681 tcaaccgtaa cgaccggtga tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag
   103741 atcgtccgtg ttgctgaact caatcaattc gcccaggtac ataaacgccg tgtggtcgga
   103801 acaacgcgca gcctgctgca tgttgtgggt gacgatcacc acggtgtaat cctgcttcag
   103861 ttcggtgatc agctcttcaa tacgcccggt agagataggg tcgagcgccg aacacggttc
   103921 gtcgagcagc agcacttccg ggcgaatggc gataccacgc gcaatacaca gacgctgttg
   103981 ctgaccacca gagagagagt aaccgctctg gtgcaattta tctttggttt cgttccacaa
   104041 tgcggctttg gtcaatgccc actgcacgcg ctcgtccatg tcggcacggg agagcttctc
   104101 aaacagacga acgccaaaag cgatgttgtc gtagatggac atcggaaacg gcgtcggttt
   104161 ctggaacacc atgcccactt tcgcacgcag cagggcgata tcctgagagt tggtcaggat
   104221 gttgtcgcca tcaagcagaa tttcaccttc cgcacgctgc tccgggtaca gttcaaacat
   104281 tttgttgaag gtacgcagca gcgtcgattt accgcagccg gacggcccga taaacgccgt
   104341 tacctggttt ttagcgatat ccaggttgat gtttttcagg gcatggaatt tgccgtagta
   104401 gaagttcaaa ttacgaacct gaattttact cggggcagtt tcaaccatac tcattgcaat
   104461 cttttcctca tcgtgcctga tgcacttcgc taatcaggct taccgtaggc cggatcaggc
   104521 gttcacgccg catccggcaa agtgttaacc gtgtttattc ttcgcaaaaa caacgcgcgc
   104581 cagaatgttc agcagcagta cgcacagggt aatgatcaat accccggccc aggccagttg
   104641 ctgccattcc gcaaacggac tcatcgcaaa cttaaagatc gtcaccggca ggttggcgat
   104701 cggctgcatc atgtccgtgc tccagaactg gttggagagc gcggtaaaca gcagcggcgc
   104761 ggtttcacca gcaatacggg caatcgccag caagataccg gtcatgatcc cggacaccga
   104821 cgctttcagc gtaatcgcgg agatcatctt ccacttcggt gtacccagcg cataagccgc
   104881 ttcacgcagg ctgtccggca ccagtttcag catgttctcg gtggtgcgga taacaatcgg
   104941 cacctgcaac agcgccagcg caattacgcc cgcccagccg gagaagtgct ccatctgtgc
   105001 caccacgatg gtgtagacaa acagaccaac cacaatcgac ggcgcggaga gcagaatgtc
   105061 gttaatgaag cgaatcactt ctgccagcca ggatttacga ccatattccg ccagataaat
   105121 ccccgccata atgcccagcg gcgtaccgaa taccgtggcc cacaaaatta acagcccgct
   105181 acccgccagg gcgttcgcca gaccaccacc ttccgtattg ggcggcggcg tcatttcagt
   105241 gaacagcgcc agcgacatac cgtcgatacc gcgagtgatg gtggacatta aaatccagat
   105301 cagccagaac aggccgaagg ccatcgtcgc catcgagagc gtcagcgcaa tacggttttt
   105361 gaggcggcga cgcgcctgca ttttgcggcg agattcagcc agcgccgcag tggtttgcat
   105421 ttcaaccata gccattagcg tgccccctca ttcttagcca ggcgcataat cataaacttc
   105481 gatgcggcga ggacgatgaa ggtaatcaca aacaggatca ggcccagttc catcagtgcg
   105541 gcaacgtgca gaccggattc cgcttccgca aattcgttcg ccagcgcaga ggtgatactg
   105601 ttgcccggca tatacagcga ggcgctgtcg agctggtagg tgttaccgat gataaaggtc
   105661 accgccatgg tttcaccgag cgcgcggccc agccccagca tgatgccgcc gataacacca
   105721 tttttggtga acggaagaac gatacgccag ataacttccc aggtggtgca gccaataccg
   105781 taggccgact ctttcatcat caccggggtt tgttcgaaca catcacgcat taccgccgca
   105841 atgtacggaa taatcatgat ggcgaggatc acgcctgccg cgaggatacc gataccaaat
   105901 gcggggccag agaacagcgc gccaacaatc gggatattcg acatgatatt gccgaccggc
   105961 tcctgaaagt aaacggcgaa cagcggcgca aagataaaca ggccccacat gccgtaaacg
   106021 atacttggaa tggctgccag cagctcaatg gcgataccca gcgggcgttt cagccagcca
   106081 ggcgcaagct cagtcaggaa cagggcgata ccgaaactca ccgggacggc gatcagcagc
   106141 gcgataaacg aagtcaccaa cgtaccgtag atcggcacca gcgccccgta gatatcgttc
   106201 ggtgcatccc actctttggt ccataggaaa gccagaccaa atttctgaat gctcggccag
   106261 gaggagatga tcagagagac aataatgcca cccaacatca atagcacaat cagcgccgcc
   106321 agttttacca gcacgctgaa aattatgtcg ccctttttac ccggtgggtt aaaagcaggc
   106381 ttggttgcag ccataagtta ctcttcagtt aaacgcgttt acaaagtcat tttgtaatgc
   106441 cggatgcggc gtaaaacacc gtacccggcc tggagtttta ttagtacagc ggcttaccgc
   106501 tactgtcttt aatattggtc ttccacgcag cgcgaacctg ttcaactaca ctatccggca
   106561 ggctggcgta atccaggtcg ttcgcctgtt tagccccggt tttgtacgcc cagtcgaaga
   106621 atttcagcac ttctgtgcct tgttctggtt tcttctgatc tttgtggatc agaatgaacg
   106681 tggtagaggt aataggccat gcatcttcgc ctttctggtt ggtcagatcc tgagcgaagg
   106741 ttttgctcca gtctgcacct tttgctgcat tagcgaagtt ttcttcggtc ggactaaccg
   106801 gtttaccatc agcggagatc agtttggtgt acgccaggtt gttctgcttc gcgtaagcat
   106861 attcaacata accaattgca cccggcagac gctgaacgaa cgcggcgata ccgtcgttac
   106921 ctttaccgcc cagaccgatc ggccatttta cggtagagcc agtaccaacg ttgtttttcc
   106981 actcttcgtt cactttcgcc aggtagctgg tgaagacgaa ggaagtcccg gagccatctg
   107041 cgcggcgtac tacagcaatg ttttgtgaag gcagtttcag acccggattc agtttggcga
   107101 tggcttcatc atcccacttc ttgattttgc ccaggtagat gtcgccgagg gttttaccat
   107161 ccagcaccag ttcgccagac ttcagccctg gaatgttaac cgccagcacc acgccgccaa
   107221 tcacggtcgg gaactggaac agaccttcct gagccagttt ttcgtcagac agcggcgcgt
   107281 cagaggcacc aaaatcaacg gtattagcga taatctgttt tacgccaccg gaagaaccga
   107341 taccctggta gttaacttta ttaccggttt ctttctggta agtgtcagcc catttggcat
   107401 acaccggcgc agggaaggtt gcacctgcac ctgtcaggct tgcttctgca aacacagaga
   107461 aagcgctcat cgataaggtc gcggcgacaa cagttgcgac ggtggtacgc ataactttca
   107521 taatgtctcc tgggaggatt cataaagcat tgtttgttgg ctacgagaag caaaatagga
   107581 caaacaggtg acagttatat gtaaggaata tgacattttt atgacagaaa ggaattacca
   107641 aataacttac tgattttaaa tgtgaagatg aaaatcccac atgtcatctt tttgtcatga
   107701 tgcatttaaa agtgataaag aaatgggtga gatgacaacg atgtgatgaa caatcgttgt
   107761 cagacctatt agcgcacaat ctcaacagtt atcgtcgctg ttgcagtaaa ttgccccggg
   107821 gatgggcgag ctccagtaag gctcgcagga gcagctgaaa aagttacact acctgatttt
   107881 tcaccataca ctgttgattt acccatgtct gttggcaact caccaccgtt cactttgatt
   107941 tccttattat ttttgtcgta aatttttatc cccacgttag agtttgaagt tacgaccatt
   108001 gaattgtccg tattgggttc ggtagcataa aaagacgtac tgatatcatc ggtattcagt
   108061 atccccaggt tttcacactc aatagtgacc gttttagtaa ttttatgtgt tgttaccgca
   108121 cttccaacaa cagatgaaaa atctgcagct ggtatttctt taaaatcaaa ataaactggc
   108181 tgtaaattat ttatttcgca actttgcgga gctgtaatat cgccccgaat ataaacatca
   108241 gccatcggct gtgagtaatc caccggggtg tcacggctaa ttgttccata caatgtcact
   108301 atctttgttt caggaataat caattttcct gcaaatgtct tcttgatata aaaatatact
   108361 atcgctttag ccccactgga tatacttaaa ggttcaatgc ggttgcaatt gtactttgta
   108421 ttaccagacg ggttgttagc atgatattca aaaggtgcat tcacataccc aaccccaaga
   108481 atatctatag caatcccaac atcaagattc ggattaagat cataataatt aatattagag
   108541 tgaaccccag cataagttaa tgcctggttt atttttgacg tataatagat ataggggaat
   108601 tctcctacgt taccagtatc acaatgacag cgtccaggat atgaggatcc attagttaca
   108661 gtggtgttaa atgtcgttcc aaccttattt tcgttcgcgg taataagata tcccgagaaa
   108721 tcaagcgtac tgtgaaatac gccattatca ggggtacaga tacctttagg agctgcaagt
   108781 gctacactac ttaacgagag cataaacaca gcaaagcacc actgtttgat atacttgttc
   108841 ataaaaatcc ttttaatgac atgtcacacc ctgttcaatt aatccgctct cactttctgg
   108901 tgtcgaaagt gaataattga agatgcattt tgttgaggcg ttttctcccc aggataacgt
   108961 aacctgtcct ttttctggta gaccagaaag ataaagttcg ccacggtcac cgacaattcc
   109021 tgaatctgcc aaacttgcat cttgtatcgc agcaatggct ccaaaaggta ccggtttacc
   109081 atttgcattt aacactctga agagaacgcg ataacccaca tgagtcacaa actccgcttt
   109141 aaccactgca ccacgcgtgg aaaccatcgt aacgacggta ttatcaagtt ctatattcga
   109201 cttcgagaaa tagttatcgc tgattgctac ccgattttca tcataagggt ttagctgtgt
   109261 cttgaccgta tagccgcgcc agtccgtcgc agcaccgcgc atattatcta tttctaaccc
   109321 ggcggcccca ggtgctttaa caagagcaat agtttcacct aactcctgac ttaacgttac
   109381 cccttcgctg tgtgccagaa ttccccctct gataccataa ttcagcctct gggaattttg
   109441 gctgtagtta tagctgccgt tcaccgtgcc ataagagcca tcataggtca gggacatatt
   109501 accgccaacc tcatcatcat gactgttata ggactgcatt aaattccatg aaaggttatt
   109561 tcgttccagc gccgtaccgc tgagtccgag ttgatgtgag ctaccggaat tatcgttttg
   109621 cgaaacagaa taggtggcgt aactattacc ccaccagccc gataaaggaa cagataaaga
   109681 gagcatgtag atctgctcac tggtgttatc actggcatgg ttgttccccg tcccctgtct
   109741 ggcagtattg gaataactcc aggagacgtt ccacgagaga cgcttccagc tactgctgta
   109801 gccaaattgc attaatcgtt cgacatcatc ggtatgccag tattgttgcc ggcttaatgt
   109861 caggtataaa ttgccataat ctctccccaa cgactgcgtt agcgttccct ccacgcgact
   109921 acgtcggtta cctgtttccc aaaagtcttc agggctgttg cgccgcgatg cccactcatt
   109981 gagggtatag tagccctcgg tggagtaacg atagccaacc aggcgcaagc tagtgtctgt
   110041 gtcgtcaaat cgttttgaat agagaaagcg atatgactga cccgtttctg tatcatcatg
   110101 gctaaaattc gcacgagcat gtgtcacatc gaaagaaatt gcgcccaaag cgccaagatc
   110161 tttaccgaca cccagagctg cagaaccata attttcagca gcctgcatac ctccatataa
   110221 agtgatcccg tgggaaaaac catgaagtat ttgcgcctga aggacgtccg gtgtaaatcc
   110281 gtcttcatcg cgcaattcac ccacgctgac atcaacatct gtctgacctt cacgtttaag
   110341 aatcgccaat gaagcataag gttgggtgaa tcgttgttcg ctaccgtctt cttctttgat
   110401 ggttacgtcc aaatcgccac ctgtactggc ggtgttgaga tcggtgatct caaaagcacc
   110461 ttgtggaacg gcagactgat aaatcacgta gccattctgc cgaatgatga cggtggcgtt
   110521 ggactttgca atcccacgaa ccacaggagc aaagccattc tggctggcgg gtagcatatc
   110581 gttatcagta tacaaacgcg cgccgcgaat ttgcgtactg tcgaaaatat cgctcgccgt
   110641 ccacgtatca ccaatcataa tctggcttct taacgcggca atatcgcgct gtatccaggt
   110701 actaatggag ttgtattccg cacggccatc gctggtgttt aaagtactat aattacgtag
   110761 ccgccacggt ccaatattca gtccattgcg cagattgaga taactggtat ctgtctcttg
   110821 tgaaccataa tctgaacgta gtgtatgtga accagaaaaa tcgtagttga tggtgagcgc
   110881 gttaatcccc tcatcccaac gcgtggggct gatgtaatca taagccatcg cgtccatgta
   110941 gatttgggga atggatatat tcagctgcaa tttatcactg tcaaactgcc aggatgagcc
   111001 ttcaatacgt ggagcaggat cgaggcaaac cgtctcatcc acatcattga acgatttgag
   111061 cgcatcaact tttatgccaa aggaggacag tgttttttta atggcaataa agcaagcgtg
   111121 cagatcagca tctggattat cctgaacaaa tgtcacatcc tgacgttcaa acattttacc
   111181 gttgataaaa atatcaacgc tataaacacc tgggggcatc aggtttgccc gattaaactg
   111241 gcgaatatcg actccggatt gatggtcgat ttccagaagc gccggattga atacactgac
   111301 ctcggccatc aggttttgcg agaaaatcag acacgtccct gccgtgaggc caaccactat
   111361 tctggtcgtc atcataatac gcctatcctt atcccttgct tacagcgtca tttttatcgg
   111421 gtcactgact gcgccgtaat cattaataaa tcgccaggtc atttggcctc ccgcagaatt
   111481 agcaggtaaa ggtaacgtga gcgtttcaaa aggacggatc atattgaggg gcacatcatg
   111541 gccatcaacg atgacatcca tcacactgat atgaattggg ttgggattct ctgctatcag
   111601 atttcgccct tcccggcgcc atttcacttt cggcgcagca tcttcgggaa taaggcggat
   111661 atttgctggt cgccagaaca gcttgatacg cgtttttact gctatttcca gcgagttcgt
   111721 cgctttgttg ctcgctgatg gcggaatatt tttaatattc agccagaaga ctgactcgcg
   111781 atcatcaggc aatgacacac ctttggtgtg aataatgcgt aatatttttt cttgcgaagg
   111841 ttctaatcgt gatactggcg gaataacggt aaacggcgct ttgcttttat cctctttatt
   111901 aaatggatct acccaagcct ggatgagata aggtattttt tcttctttgc taaatacaga
   111961 aatagattta tcagcattat tacttaagta aaccacccgc gtaccaccaa tgacaacgcc
   112021 tgcataagtt aatgctggtt ggcctaaaaa aacgctgagt ataatcgcgg taaataactt
   112081 acatgaaact gttgttttaa agagcctgat taaacaactt tttctggaca taacgactcc
   112141 ctggccaaag ccaactgaat aaaaaggccc ttgataaatt atttcttata ttcgacagta
   112201 aattgtgaat cagcgttcgc cgtaccagcc gttacggtcg cattggttgc cacatagcgt
   112261 gcaataaact tcatttcagc cttgccttca gcatcgatag cggtataagc agagtcgtta
   112321 taaagtttaa cctgcgaatt atcggactgg ttaaaaatac gaataccaac gccggttgcc
   112381 gcaatcgctt gtcctgtacc ggtaaaatca ccagggttgc ctgctgtaag cggcgtaccc
   112441 actttcaggt cgccgtcgcc gccaggcgct tctgtaccat caaaacgaac ggctgcttga
   112501 gtgtacgttg ccgggcaatt ttcaagctta atactgaact tctgtggcga cgccagacta
   112561 cctacacctg aaaatgcatt tttactgact ttacccagtg ggacaatttg atcggctgta
   112621 tcggaggtaa cttcacaagt agtgtcgaca atttcaccga cgaagtgaac aacaccatct
   112681 tctgccagtg catgcccggc cagcattaga gatgccagag tgaatgcacc gcc
//
LOCUS       DAZFWL020000002       105128 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFWL020000002 DAZFWL020000000
VERSION     DAZFWL020000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 105128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 105128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 105128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..105128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.002"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(138..338)
                     /locus_tag="ACHKNP_RS00555"
                     /old_locus_tag="pgaptmp_000111"
     CDS             complement(138..338)
                     /locus_tag="ACHKNP_RS00555"
                     /old_locus_tag="pgaptmp_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702359.1"
                     /translation="MIDPRTPIGRATLRYRGLPTRHLLSLLRLGVDNPDRPYYSRDEL
                     IAMLVDRDLNNQLRRAFAKLES"
     gene            complement(340..1068)
                     /locus_tag="ACHKNP_RS00560"
                     /old_locus_tag="pgaptmp_000112"
     CDS             complement(340..1068)
                     /locus_tag="ACHKNP_RS00560"
                     /old_locus_tag="pgaptmp_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4702360.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPAIVDKLRGILIA
                     FTGTDQQKQEHLLKQAQSGKLEGL"
     gene            complement(1977..2636)
                     /locus_tag="ACHKNP_RS00565"
                     /old_locus_tag="pgaptmp_000113"
     CDS             complement(1977..2636)
                     /locus_tag="ACHKNP_RS00565"
                     /old_locus_tag="pgaptmp_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HGU4702361.1"
                     /translation="MAEYYPAVFEAEAFNCPHCGVYARQFWRRMYGSARELAQRTTEF
                     RMSTCSHCGDDAYWYDGNMIIPAAGNVELPNPDMPDNCKSDYMEARSIINLSPKGAAA
                     LLRLCLQKLMVHLGEPGENINKDIRSLVQKGLPVRIQQAADICRIVGNQAVHPGEISL
                     DDDPQLAHGLFKLLNIIVDDRITRPKEIEAMFQSMPEGPRQGIENQDRQAREQQQAAN
                     E"
     gene            complement(2629..4236)
                     /locus_tag="ACHKNP_RS00570"
                     /old_locus_tag="pgaptmp_000114"
     CDS             complement(2629..4236)
                     /locus_tag="ACHKNP_RS00570"
                     /old_locus_tag="pgaptmp_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4702362.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPSASYEELREHLKTIVEWELGVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLKGDNSGLLSYLE
                     PETGSLRPSVSLSVSSEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTAGKPYHYYVTALAAITGARLNEVAQLQVKDVRATEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTAGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGLNG"
     gene            complement(4371..4444)
                     /locus_tag="ACHKNP_RS00575"
                     /old_locus_tag="pgaptmp_000115"
     tRNA            complement(4371..4444)
                     /locus_tag="ACHKNP_RS00575"
                     /old_locus_tag="pgaptmp_000115"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4410..4412),aa:Gly,seq:ccc)
     gene            complement(4523..5278)
                     /gene="actS"
                     /locus_tag="ACHKNP_RS00580"
                     /old_locus_tag="pgaptmp_000116"
     CDS             complement(4523..5278)
                     /gene="actS"
                     /locus_tag="ACHKNP_RS00580"
                     /old_locus_tag="pgaptmp_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HGU4702363.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            5693..7990
                     /gene="xdhA"
                     /locus_tag="ACHKNP_RS00585"
                     /old_locus_tag="pgaptmp_000117"
     CDS             5693..7990
                     /gene="xdhA"
                     /locus_tag="ACHKNP_RS00585"
                     /old_locus_tag="pgaptmp_000117"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HGU4702364.1"
                     /translation="MEAREATATGESCMRVNAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            8001..8879
                     /gene="xdhB"
                     /locus_tag="ACHKNP_RS00590"
                     /old_locus_tag="pgaptmp_000118"
     CDS             8001..8879
                     /gene="xdhB"
                     /locus_tag="ACHKNP_RS00590"
                     /old_locus_tag="pgaptmp_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HGU4702365.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            8876..9355
                     /gene="xdhC"
                     /locus_tag="ACHKNP_RS00595"
                     /old_locus_tag="pgaptmp_000119"
     CDS             8876..9355
                     /gene="xdhC"
                     /locus_tag="ACHKNP_RS00595"
                     /old_locus_tag="pgaptmp_000119"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HGU4702366.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(9395..11173)
                     /gene="ygeV"
                     /locus_tag="ACHKNP_RS00600"
                     /old_locus_tag="pgaptmp_000120"
     CDS             complement(9395..11173)
                     /gene="ygeV"
                     /locus_tag="ACHKNP_RS00600"
                     /old_locus_tag="pgaptmp_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGU4702367.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            11652..12839
                     /gene="ygeW"
                     /locus_tag="ACHKNP_RS00605"
                     /old_locus_tag="pgaptmp_000121"
     CDS             11652..12839
                     /gene="ygeW"
                     /locus_tag="ACHKNP_RS00605"
                     /old_locus_tag="pgaptmp_000121"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HGU4702368.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            12897..14093
                     /gene="dpaL"
                     /locus_tag="ACHKNP_RS00610"
                     /old_locus_tag="pgaptmp_000122"
     CDS             12897..14093
                     /gene="dpaL"
                     /locus_tag="ACHKNP_RS00610"
                     /old_locus_tag="pgaptmp_000122"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HGU4702369.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            14151..15362
                     /gene="ygeY"
                     /locus_tag="ACHKNP_RS00615"
                     /old_locus_tag="pgaptmp_000123"
     CDS             14151..15362
                     /gene="ygeY"
                     /locus_tag="ACHKNP_RS00615"
                     /old_locus_tag="pgaptmp_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HGU4702370.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            15415..16800
                     /gene="hyuA"
                     /locus_tag="ACHKNP_RS00620"
                     /old_locus_tag="pgaptmp_000124"
     CDS             15415..16800
                     /gene="hyuA"
                     /locus_tag="ACHKNP_RS00620"
                     /old_locus_tag="pgaptmp_000124"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HGU4702371.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            16848..17780
                     /gene="arcC"
                     /locus_tag="ACHKNP_RS00625"
                     /old_locus_tag="pgaptmp_000125"
     CDS             16848..17780
                     /gene="arcC"
                     /locus_tag="ACHKNP_RS00625"
                     /old_locus_tag="pgaptmp_000125"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU4702372.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALLDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(17821..19446)
                     /gene="yqeB"
                     /locus_tag="ACHKNP_RS00630"
                     /old_locus_tag="pgaptmp_000126"
     CDS             complement(17821..19446)
                     /gene="yqeB"
                     /locus_tag="ACHKNP_RS00630"
                     /old_locus_tag="pgaptmp_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HGU4702373.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSTA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(19494..20264)
                     /gene="yqeC"
                     /locus_tag="ACHKNP_RS00635"
                     /old_locus_tag="pgaptmp_000127"
     CDS             complement(19494..20264)
                     /gene="yqeC"
                     /locus_tag="ACHKNP_RS00635"
                     /old_locus_tag="pgaptmp_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HGU4702374.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            20367..20945
                     /gene="mocA"
                     /locus_tag="ACHKNP_RS00640"
                     /old_locus_tag="pgaptmp_000128"
     CDS             20367..20945
                     /gene="mocA"
                     /locus_tag="ACHKNP_RS00640"
                     /old_locus_tag="pgaptmp_000128"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HGU4702375.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            21267..24365
                     /gene="ygfK"
                     /locus_tag="ACHKNP_RS00645"
                     /old_locus_tag="pgaptmp_000129"
     CDS             21267..24365
                     /gene="ygfK"
                     /locus_tag="ACHKNP_RS00645"
                     /old_locus_tag="pgaptmp_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HGU4702376.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            24368..25696
                     /gene="ssnA"
                     /locus_tag="ACHKNP_RS00650"
                     /old_locus_tag="pgaptmp_000130"
     CDS             24368..25696
                     /gene="ssnA"
                     /locus_tag="ACHKNP_RS00650"
                     /old_locus_tag="pgaptmp_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HGU4702377.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            25747..26526
                     /gene="ygfM"
                     /locus_tag="ACHKNP_RS00655"
                     /old_locus_tag="pgaptmp_000131"
     CDS             25747..26526
                     /gene="ygfM"
                     /locus_tag="ACHKNP_RS00655"
                     /old_locus_tag="pgaptmp_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HGU4702378.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            26523..29393
                     /gene="xdhD"
                     /locus_tag="ACHKNP_RS00660"
                     /old_locus_tag="pgaptmp_000132"
     CDS             26523..29393
                     /gene="xdhD"
                     /locus_tag="ACHKNP_RS00660"
                     /old_locus_tag="pgaptmp_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HGU4702379.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYNAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            29558..30958
                     /gene="xanQ"
                     /locus_tag="ACHKNP_RS00665"
                     /old_locus_tag="pgaptmp_000133"
     CDS             29558..30958
                     /gene="xanQ"
                     /locus_tag="ACHKNP_RS00665"
                     /old_locus_tag="pgaptmp_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HGU4702380.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            30976..32292
                     /gene="guaD"
                     /locus_tag="ACHKNP_RS00670"
                     /old_locus_tag="pgaptmp_000134"
     CDS             30976..32292
                     /gene="guaD"
                     /locus_tag="ACHKNP_RS00670"
                     /old_locus_tag="pgaptmp_000134"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HGU4702381.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWARLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            32328..33695
                     /gene="ghxQ"
                     /locus_tag="ACHKNP_RS00675"
                     /old_locus_tag="pgaptmp_000135"
                     /gene_synonym="ygfQ"
     CDS             32328..33695
                     /gene="ghxQ"
                     /locus_tag="ACHKNP_RS00675"
                     /old_locus_tag="pgaptmp_000135"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HGU4702382.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(33731..34219)
                     /gene="ygfS"
                     /locus_tag="ACHKNP_RS00680"
                     /old_locus_tag="pgaptmp_000136"
     CDS             complement(33731..34219)
                     /gene="ygfS"
                     /locus_tag="ACHKNP_RS00680"
                     /old_locus_tag="pgaptmp_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU4702383.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(34219..36138)
                     /gene="ygfT"
                     /locus_tag="ACHKNP_RS00685"
                     /old_locus_tag="pgaptmp_000137"
     CDS             complement(34219..36138)
                     /gene="ygfT"
                     /locus_tag="ACHKNP_RS00685"
                     /old_locus_tag="pgaptmp_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HGU4702384.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            36574..38022
                     /gene="uacT"
                     /locus_tag="ACHKNP_RS00690"
                     /old_locus_tag="pgaptmp_000138"
     CDS             36574..38022
                     /gene="uacT"
                     /locus_tag="ACHKNP_RS00690"
                     /old_locus_tag="pgaptmp_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HGU4702385.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            38024..38149
                     /gene="yqfG"
                     /locus_tag="ACHKNP_RS00695"
                     /old_locus_tag="pgaptmp_000139"
     CDS             38024..38149
                     /gene="yqfG"
                     /locus_tag="ACHKNP_RS00695"
                     /old_locus_tag="pgaptmp_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HGU4702386.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(38146..38217)
                     /locus_tag="ACHKNP_RS00700"
                     /old_locus_tag="pgaptmp_000140"
     CDS             complement(38146..38217)
                     /locus_tag="ACHKNP_RS00700"
                     /old_locus_tag="pgaptmp_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702387.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            38272..38820
                     /gene="idi"
                     /locus_tag="ACHKNP_RS00705"
                     /old_locus_tag="pgaptmp_000141"
     CDS             38272..38820
                     /gene="idi"
                     /locus_tag="ACHKNP_RS00705"
                     /old_locus_tag="pgaptmp_000141"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGU4702388.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHRAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYGLGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(38863..40380)
                     /gene="lysS"
                     /locus_tag="ACHKNP_RS00710"
                     /old_locus_tag="pgaptmp_000142"
     CDS             complement(38863..40380)
                     /gene="lysS"
                     /locus_tag="ACHKNP_RS00710"
                     /old_locus_tag="pgaptmp_000142"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU4702389.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGVAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(40390..41488)
                     /gene="prfB"
                     /locus_tag="ACHKNP_RS00715"
                     /old_locus_tag="pgaptmp_000143"
     CDS             complement(join(40390..41412,41414..41488))
                     /gene="prfB"
                     /locus_tag="ACHKNP_RS00715"
                     /old_locus_tag="pgaptmp_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGU4702390.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(41579..43312)
                     /gene="recJ"
                     /locus_tag="ACHKNP_RS00720"
                     /old_locus_tag="pgaptmp_000144"
     CDS             complement(41579..43312)
                     /gene="recJ"
                     /locus_tag="ACHKNP_RS00720"
                     /old_locus_tag="pgaptmp_000144"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGU4702391.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(43318..44028)
                     /gene="dsbC"
                     /locus_tag="ACHKNP_RS00725"
                     /old_locus_tag="pgaptmp_000145"
     CDS             complement(43318..44028)
                     /gene="dsbC"
                     /locus_tag="ACHKNP_RS00725"
                     /old_locus_tag="pgaptmp_000145"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HGU4702392.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(44053..44949)
                     /gene="xerD"
                     /locus_tag="ACHKNP_RS00730"
                     /old_locus_tag="pgaptmp_000146"
     CDS             complement(44053..44949)
                     /gene="xerD"
                     /locus_tag="ACHKNP_RS00730"
                     /old_locus_tag="pgaptmp_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGU4702393.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLRAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            45061..45582
                     /gene="fldB"
                     /locus_tag="ACHKNP_RS00735"
                     /old_locus_tag="pgaptmp_000147"
     CDS             45061..45582
                     /gene="fldB"
                     /locus_tag="ACHKNP_RS00735"
                     /old_locus_tag="pgaptmp_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HGU4702394.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(45622..46029)
                     /gene="ygfX"
                     /locus_tag="ACHKNP_RS00740"
                     /old_locus_tag="pgaptmp_000148"
     CDS             complement(45622..46029)
                     /gene="ygfX"
                     /locus_tag="ACHKNP_RS00740"
                     /old_locus_tag="pgaptmp_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HGU4702395.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(46010..46276)
                     /gene="sdhE"
                     /locus_tag="ACHKNP_RS00745"
                     /old_locus_tag="pgaptmp_000149"
     CDS             complement(46010..46276)
                     /gene="sdhE"
                     /locus_tag="ACHKNP_RS00745"
                     /old_locus_tag="pgaptmp_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HGU4702396.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            46519..47499
                     /gene="ygfZ"
                     /locus_tag="ACHKNP_RS00750"
                     /old_locus_tag="pgaptmp_000150"
     CDS             46519..47499
                     /gene="ygfZ"
                     /locus_tag="ACHKNP_RS00750"
                     /old_locus_tag="pgaptmp_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HGU4702397.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(47695..48354)
                     /gene="yqfA"
                     /locus_tag="ACHKNP_RS00755"
                     /old_locus_tag="pgaptmp_000151"
     CDS             complement(47695..48354)
                     /gene="yqfA"
                     /locus_tag="ACHKNP_RS00755"
                     /old_locus_tag="pgaptmp_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGU4702398.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(48518..48829)
                     /gene="yqfB"
                     /locus_tag="ACHKNP_RS00760"
                     /old_locus_tag="pgaptmp_000152"
     CDS             complement(48518..48829)
                     /gene="yqfB"
                     /locus_tag="ACHKNP_RS00760"
                     /old_locus_tag="pgaptmp_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HGU4702399.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            48874..50307
                     /gene="bglA"
                     /locus_tag="ACHKNP_RS00765"
                     /old_locus_tag="pgaptmp_000153"
     CDS             48874..50307
                     /gene="bglA"
                     /locus_tag="ACHKNP_RS00765"
                     /old_locus_tag="pgaptmp_000153"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HGU4702400.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRIHPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            complement(50364..51107)
                     /gene="ygfF"
                     /locus_tag="ACHKNP_RS00770"
                     /old_locus_tag="pgaptmp_000154"
     CDS             complement(50364..51107)
                     /gene="ygfF"
                     /locus_tag="ACHKNP_RS00770"
                     /old_locus_tag="pgaptmp_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4702401.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYTASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(51368..54241)
                     /gene="gcvP"
                     /locus_tag="ACHKNP_RS00775"
                     /old_locus_tag="pgaptmp_000155"
     CDS             complement(51368..54241)
                     /gene="gcvP"
                     /locus_tag="ACHKNP_RS00775"
                     /old_locus_tag="pgaptmp_000155"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HGU4702402.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(54360..54749)
                     /gene="gcvH"
                     /locus_tag="ACHKNP_RS00780"
                     /old_locus_tag="pgaptmp_000156"
     CDS             complement(54360..54749)
                     /gene="gcvH"
                     /locus_tag="ACHKNP_RS00780"
                     /old_locus_tag="pgaptmp_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HGU4702403.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(54773..55867)
                     /gene="gcvT"
                     /locus_tag="ACHKNP_RS00785"
                     /old_locus_tag="pgaptmp_000157"
     CDS             complement(54773..55867)
                     /gene="gcvT"
                     /locus_tag="ACHKNP_RS00785"
                     /old_locus_tag="pgaptmp_000157"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HGU4702404.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            56158..56241
                     /locus_tag="ACHKNP_RS00790"
                     /old_locus_tag="pgaptmp_000158"
     CDS             56158..56241
                     /locus_tag="ACHKNP_RS00790"
                     /old_locus_tag="pgaptmp_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702405.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            complement(56315..57517)
                     /gene="ubiI"
                     /locus_tag="ACHKNP_RS00795"
                     /old_locus_tag="pgaptmp_000159"
                     /gene_synonym="visC"
     CDS             complement(56315..57517)
                     /gene="ubiI"
                     /locus_tag="ACHKNP_RS00795"
                     /old_locus_tag="pgaptmp_000159"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HGU4702406.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(57540..58718)
                     /gene="ubiH"
                     /locus_tag="ACHKNP_RS00800"
                     /old_locus_tag="pgaptmp_000160"
                     /gene_synonym="visB"
     CDS             complement(57540..58718)
                     /gene="ubiH"
                     /locus_tag="ACHKNP_RS00800"
                     /old_locus_tag="pgaptmp_000160"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HGU4702407.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(58715..60040)
                     /gene="pepP"
                     /locus_tag="ACHKNP_RS00805"
                     /old_locus_tag="pgaptmp_000161"
     CDS             complement(58715..60040)
                     /gene="pepP"
                     /locus_tag="ACHKNP_RS00805"
                     /old_locus_tag="pgaptmp_000161"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HGU4702408.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(60066..60644)
                     /gene="ygfB"
                     /locus_tag="ACHKNP_RS00810"
                     /old_locus_tag="pgaptmp_000162"
     CDS             complement(60066..60644)
                     /gene="ygfB"
                     /locus_tag="ACHKNP_RS00810"
                     /old_locus_tag="pgaptmp_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HGU4702409.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            60812..61141
                     /gene="zapA"
                     /locus_tag="ACHKNP_RS00815"
                     /old_locus_tag="pgaptmp_000163"
     CDS             60812..61141
                     /gene="zapA"
                     /locus_tag="ACHKNP_RS00815"
                     /old_locus_tag="pgaptmp_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU4702410.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            61183..61366
                     /gene="ssrS"
                     /locus_tag="ACHKNP_RS00820"
                     /old_locus_tag="pgaptmp_000164"
     ncRNA           61183..61366
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKNP_RS00820"
                     /old_locus_tag="pgaptmp_000164"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            61387..61989
                     /gene="fau"
                     /locus_tag="ACHKNP_RS00825"
                     /old_locus_tag="pgaptmp_000165"
     CDS             61387..61989
                     /gene="fau"
                     /locus_tag="ACHKNP_RS00825"
                     /old_locus_tag="pgaptmp_000165"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGU4702411.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(62090..62149)
                     /locus_tag="ACHKNP_RS00830"
                     /old_locus_tag="pgaptmp_000166"
     CDS             complement(62090..62149)
                     /locus_tag="ACHKNP_RS00830"
                     /old_locus_tag="pgaptmp_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4702412.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(62482..62541)
                     /gene="ibsC"
                     /locus_tag="ACHKNP_RS00835"
                     /old_locus_tag="pgaptmp_000167"
     CDS             complement(62482..62541)
                     /gene="ibsC"
                     /locus_tag="ACHKNP_RS00835"
                     /old_locus_tag="pgaptmp_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HGU4702413.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(62770..64002)
                     /gene="serA"
                     /locus_tag="ACHKNP_RS00840"
                     /old_locus_tag="pgaptmp_000168"
     CDS             complement(62770..64002)
                     /gene="serA"
                     /locus_tag="ACHKNP_RS00840"
                     /old_locus_tag="pgaptmp_000168"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGU4702414.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(64258..64917)
                     /gene="rpiA"
                     /locus_tag="ACHKNP_RS00845"
                     /old_locus_tag="pgaptmp_000169"
     CDS             complement(64258..64917)
                     /gene="rpiA"
                     /locus_tag="ACHKNP_RS00845"
                     /old_locus_tag="pgaptmp_000169"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGU4702415.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(64973..65158)
                     /locus_tag="ACHKNP_RS00850"
                     /old_locus_tag="pgaptmp_000170"
     CDS             complement(64973..65158)
                     /locus_tag="ACHKNP_RS00850"
                     /old_locus_tag="pgaptmp_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702416.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            65300..66193
                     /gene="argP"
                     /locus_tag="ACHKNP_RS00855"
                     /old_locus_tag="pgaptmp_000171"
     CDS             65300..66193
                     /gene="argP"
                     /locus_tag="ACHKNP_RS00855"
                     /old_locus_tag="pgaptmp_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HGU4702417.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            66397..68541
                     /gene="scpA"
                     /locus_tag="ACHKNP_RS00860"
                     /old_locus_tag="pgaptmp_000172"
     CDS             66397..68541
                     /gene="scpA"
                     /locus_tag="ACHKNP_RS00860"
                     /old_locus_tag="pgaptmp_000172"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HGU4702418.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            68534..69529
                     /gene="meaB"
                     /locus_tag="ACHKNP_RS00865"
                     /old_locus_tag="pgaptmp_000173"
     CDS             68534..69529
                     /gene="meaB"
                     /locus_tag="ACHKNP_RS00865"
                     /old_locus_tag="pgaptmp_000173"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HGU4702419.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            69540..70325
                     /gene="scpB"
                     /locus_tag="ACHKNP_RS00870"
                     /old_locus_tag="pgaptmp_000174"
     CDS             69540..70325
                     /gene="scpB"
                     /locus_tag="ACHKNP_RS00870"
                     /old_locus_tag="pgaptmp_000174"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HGU4702420.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAQLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            70349..71827
                     /gene="scpC"
                     /locus_tag="ACHKNP_RS00875"
                     /old_locus_tag="pgaptmp_000175"
     CDS             70349..71827
                     /gene="scpC"
                     /locus_tag="ACHKNP_RS00875"
                     /old_locus_tag="pgaptmp_000175"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HGU4702421.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(71824..72720)
                     /gene="ygfI"
                     /locus_tag="ACHKNP_RS00880"
                     /old_locus_tag="pgaptmp_000176"
     CDS             complement(71824..72720)
                     /gene="ygfI"
                     /locus_tag="ACHKNP_RS00880"
                     /old_locus_tag="pgaptmp_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4702422.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(72887..73627)
                     /gene="yggE"
                     /locus_tag="ACHKNP_RS00885"
                     /old_locus_tag="pgaptmp_000177"
     CDS             complement(72887..73627)
                     /gene="yggE"
                     /locus_tag="ACHKNP_RS00885"
                     /old_locus_tag="pgaptmp_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HGU4702423.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(73720..74355)
                     /gene="argO"
                     /locus_tag="ACHKNP_RS00890"
                     /old_locus_tag="pgaptmp_000178"
     CDS             complement(73720..74355)
                     /gene="argO"
                     /locus_tag="ACHKNP_RS00890"
                     /old_locus_tag="pgaptmp_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HGU4702424.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(74494..75354)
                     /gene="mscS"
                     /locus_tag="ACHKNP_RS00895"
                     /old_locus_tag="pgaptmp_000179"
     CDS             complement(74494..75354)
                     /gene="mscS"
                     /locus_tag="ACHKNP_RS00895"
                     /old_locus_tag="pgaptmp_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HGU4702425.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            75550..75648
                     /locus_tag="ACHKNP_RS00900"
                     /old_locus_tag="pgaptmp_000180"
                     /pseudo
     CDS             75550..75648
                     /locus_tag="ACHKNP_RS00900"
                     /old_locus_tag="pgaptmp_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(75712..76791)
                     /gene="fbaA"
                     /locus_tag="ACHKNP_RS00905"
                     /old_locus_tag="pgaptmp_000181"
     CDS             complement(75712..76791)
                     /gene="fbaA"
                     /locus_tag="ACHKNP_RS00905"
                     /old_locus_tag="pgaptmp_000181"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGU4702426.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(77006..78169)
                     /gene="pgk"
                     /locus_tag="ACHKNP_RS00910"
                     /old_locus_tag="pgaptmp_000182"
     CDS             complement(77006..78169)
                     /gene="pgk"
                     /locus_tag="ACHKNP_RS00910"
                     /old_locus_tag="pgaptmp_000182"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGU4702427.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(78219..79238)
                     /gene="epd"
                     /locus_tag="ACHKNP_RS00915"
                     /old_locus_tag="pgaptmp_000183"
     CDS             complement(78219..79238)
                     /gene="epd"
                     /locus_tag="ACHKNP_RS00915"
                     /old_locus_tag="pgaptmp_000183"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HGU4702428.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            79610..80041
                     /locus_tag="ACHKNP_RS00920"
                     /old_locus_tag="pgaptmp_000184"
     CDS             79610..80041
                     /locus_tag="ACHKNP_RS00920"
                     /old_locus_tag="pgaptmp_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HGU4702429.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            80064..80642
                     /locus_tag="ACHKNP_RS00925"
                     /old_locus_tag="pgaptmp_000185"
     CDS             80064..80642
                     /locus_tag="ACHKNP_RS00925"
                     /old_locus_tag="pgaptmp_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGU4702430.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            80643..81350
                     /locus_tag="ACHKNP_RS00930"
                     /old_locus_tag="pgaptmp_000186"
     CDS             80643..81350
                     /locus_tag="ACHKNP_RS00930"
                     /old_locus_tag="pgaptmp_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGU4702431.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATQ
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            81338..82015
                     /locus_tag="ACHKNP_RS00935"
                     /old_locus_tag="pgaptmp_000187"
     CDS             81338..82015
                     /locus_tag="ACHKNP_RS00935"
                     /old_locus_tag="pgaptmp_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4702432.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            82009..82686
                     /locus_tag="ACHKNP_RS00940"
                     /old_locus_tag="pgaptmp_000188"
     CDS             82009..82686
                     /locus_tag="ACHKNP_RS00940"
                     /old_locus_tag="pgaptmp_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4702433.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(82658..83371)
                     /locus_tag="ACHKNP_RS00945"
                     /old_locus_tag="pgaptmp_000189"
     CDS             complement(82658..83371)
                     /locus_tag="ACHKNP_RS00945"
                     /old_locus_tag="pgaptmp_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HGU4702434.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(83368..83877)
                     /gene="fumE"
                     /locus_tag="ACHKNP_RS00950"
                     /old_locus_tag="pgaptmp_000190"
     CDS             complement(83368..83877)
                     /gene="fumE"
                     /locus_tag="ACHKNP_RS00950"
                     /old_locus_tag="pgaptmp_000190"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HGU4702435.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(83899..84864)
                     /gene="glpX"
                     /locus_tag="ACHKNP_RS00955"
                     /old_locus_tag="pgaptmp_000191"
     CDS             complement(83899..84864)
                     /gene="glpX"
                     /locus_tag="ACHKNP_RS00955"
                     /old_locus_tag="pgaptmp_000191"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU4702436.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(84861..86138)
                     /gene="yggP"
                     /locus_tag="ACHKNP_RS00960"
                     /old_locus_tag="pgaptmp_000192"
     CDS             complement(84861..86138)
                     /gene="yggP"
                     /locus_tag="ACHKNP_RS00960"
                     /old_locus_tag="pgaptmp_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU4702437.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(86153..87541)
                     /gene="cmtA"
                     /locus_tag="ACHKNP_RS00965"
                     /old_locus_tag="pgaptmp_000193"
     CDS             complement(86153..87541)
                     /gene="cmtA"
                     /locus_tag="ACHKNP_RS00965"
                     /old_locus_tag="pgaptmp_000193"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HGU4702438.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(87569..88012)
                     /gene="cmtB"
                     /locus_tag="ACHKNP_RS00970"
                     /old_locus_tag="pgaptmp_000194"
     CDS             complement(87569..88012)
                     /gene="cmtB"
                     /locus_tag="ACHKNP_RS00970"
                     /old_locus_tag="pgaptmp_000194"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HGU4702439.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(88326..90317)
                     /gene="tkt"
                     /locus_tag="ACHKNP_RS00975"
                     /old_locus_tag="pgaptmp_000195"
     CDS             complement(88326..90317)
                     /gene="tkt"
                     /locus_tag="ACHKNP_RS00975"
                     /old_locus_tag="pgaptmp_000195"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU4702440.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            90595..91353
                     /gene="loiP"
                     /locus_tag="ACHKNP_RS00980"
                     /old_locus_tag="pgaptmp_000196"
                     /gene_synonym="yggG"
     CDS             90595..91353
                     /gene="loiP"
                     /locus_tag="ACHKNP_RS00980"
                     /old_locus_tag="pgaptmp_000196"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HGU4702441.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(91409..92152)
                     /locus_tag="ACHKNP_RS00985"
                     /old_locus_tag="pgaptmp_000197"
     CDS             complement(91409..92152)
                     /locus_tag="ACHKNP_RS00985"
                     /old_locus_tag="pgaptmp_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGU4702442.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(92139..93248)
                     /gene="dgaE"
                     /locus_tag="ACHKNP_RS00990"
                     /old_locus_tag="pgaptmp_000198"
     CDS             complement(92139..93248)
                     /gene="dgaE"
                     /locus_tag="ACHKNP_RS00990"
                     /old_locus_tag="pgaptmp_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HGU4702443.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(93252..94112)
                     /locus_tag="ACHKNP_RS00995"
                     /old_locus_tag="pgaptmp_000199"
     CDS             complement(93252..94112)
                     /locus_tag="ACHKNP_RS00995"
                     /old_locus_tag="pgaptmp_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGU4702444.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(94109..94858)
                     /locus_tag="ACHKNP_RS01000"
                     /old_locus_tag="pgaptmp_000200"
     CDS             complement(94109..94858)
                     /locus_tag="ACHKNP_RS01000"
                     /old_locus_tag="pgaptmp_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGU4702445.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(94884..95369)
                     /locus_tag="ACHKNP_RS01005"
                     /old_locus_tag="pgaptmp_000201"
     CDS             complement(94884..95369)
                     /locus_tag="ACHKNP_RS01005"
                     /old_locus_tag="pgaptmp_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGU4702446.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(95380..95808)
                     /locus_tag="ACHKNP_RS01010"
                     /old_locus_tag="pgaptmp_000202"
     CDS             complement(95380..95808)
                     /locus_tag="ACHKNP_RS01010"
                     /old_locus_tag="pgaptmp_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU4702447.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(95927..98725)
                     /gene="dagR"
                     /locus_tag="ACHKNP_RS01015"
                     /old_locus_tag="pgaptmp_000203"
     CDS             complement(95927..98725)
                     /gene="dagR"
                     /locus_tag="ACHKNP_RS01015"
                     /old_locus_tag="pgaptmp_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HGU4702448.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(98984..99904)
                     /gene="speB"
                     /locus_tag="ACHKNP_RS01020"
                     /old_locus_tag="pgaptmp_000204"
     CDS             complement(98984..99904)
                     /gene="speB"
                     /locus_tag="ACHKNP_RS01020"
                     /old_locus_tag="pgaptmp_000204"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HGU4702449.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(100040..100771)
                     /locus_tag="ACHKNP_RS01025"
                     /old_locus_tag="pgaptmp_000205"
     CDS             complement(100040..100771)
                     /locus_tag="ACHKNP_RS01025"
                     /old_locus_tag="pgaptmp_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702450.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(100917..102893)
                     /gene="speA"
                     /locus_tag="ACHKNP_RS01030"
                     /old_locus_tag="pgaptmp_000206"
     CDS             complement(100917..102893)
                     /gene="speA"
                     /locus_tag="ACHKNP_RS01030"
                     /old_locus_tag="pgaptmp_000206"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HGU4702451.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(102902..103033)
                     /gene="yqgB"
                     /locus_tag="ACHKNP_RS01035"
                     /old_locus_tag="pgaptmp_000207"
     CDS             complement(102902..103033)
                     /gene="yqgB"
                     /locus_tag="ACHKNP_RS01035"
                     /old_locus_tag="pgaptmp_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HGU4702452.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(103127..103270)
                     /gene="yqgG"
                     /locus_tag="ACHKNP_RS01040"
                     /old_locus_tag="pgaptmp_000208"
     CDS             complement(103127..103270)
                     /gene="yqgG"
                     /locus_tag="ACHKNP_RS01040"
                     /old_locus_tag="pgaptmp_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HGU4702453.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(103381..103632)
                     /gene="yqgD"
                     /locus_tag="ACHKNP_RS01045"
                     /old_locus_tag="pgaptmp_000209"
     CDS             complement(103381..103632)
                     /gene="yqgD"
                     /locus_tag="ACHKNP_RS01045"
                     /old_locus_tag="pgaptmp_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HGU4702454.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            103688..104842
                     /gene="metK"
                     /locus_tag="ACHKNP_RS01050"
                     /old_locus_tag="pgaptmp_000210"
     CDS             103688..104842
                     /gene="metK"
                     /locus_tag="ACHKNP_RS01050"
                     /old_locus_tag="pgaptmp_000210"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGU4702455.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT        25637 a        27979 c        26190 g        25322 t
ORIGIN      
        1 tagacatagt aattactcct cttgagagtt gagactatcg gtatcgcaga cacagtaatg
       61 cctacgggtc tccccgcatc ggtcatctca aaaggtttta aataaggttg gttaaggtct
      121 ggaaatttcc ggagatgtta agactccagt ttcgcaaagg ctcgacgtaa ctgattattc
      181 aaatcacggt cgaccagcat cgctatcagc tcgtctcgac tgtagtaagg gcgatcaggg
      241 ttatcgactc caagtctcag cagggacagg aggtgacggg taggcagacc acggtagcgg
      301 agagttgcac gaccaatagg tgtgcgcggg tcaatcatct tagagtccct ccagcttgcc
      361 ggattgggct tgcttcagga gatgctcttg cttctgctgg tcggtgccag tgaaggcaat
      421 aaggatgcct cgcagtttat cgactatcgc aggcttaccg aagacctcca atcccttagc
      481 gttgaagcga aagttataac cacctgctga gaacttctgg tctgacggct taatgatacg
      541 gaagcagtcg gaatactttg gatttacaga ccactcgtta ctgattgggt tcttacgaaa
      601 gagaccacga tgctgaagaa gtgcggtcat tagcttctga gcgggtagac cataggtttt
      661 gcagtagtcg ccccaagtca cgcctcggcg gttagcggac tgaatggctg ctcgcttctt
      721 ctggtcttcc agttgaagga ctcgacgctc taacgtctga atatggtcgt aacacttggc
      781 gcggaggtga acggaatagc cagcgaccag aatgaaagta tgcttacggt cgagacggta
      841 cagaggctgc tgtttcccct gcgcgtttag atagacatct tcagtcaccc cgatatgact
      901 gctcagtttt gagcactgct cagttttgag cgcgttaagc tcctcagcca tattgcgaat
      961 atcacgtagg acatttcggt gctccttgcc tgtcagctca gcaatttcaa ctgaggtcat
     1021 ggagacaagc tggtcgaggt taatgacgct gatttcttgt tgagacattt acgtgtactc
     1081 cttttgtttg caccctgaag tcgcaccccc actgacctac tcaagccccc acgtaaaggg
     1141 agaccagcag gcaacaggac gcaaacagaa tgacttgagg taattggggt tagtggtctg
     1201 cgatgaccat agagatgaag gttcgacagc atgtccgagc gtgtgtggtg atagacttaa
     1261 gtcacgagac caaagaacac aaaggctgac cagaggctgg tggctagcaa gctagcgtgg
     1321 accacggttt ctcgcttgcc gtctctcagg aggtcagagg cgagatatgg tccctatagt
     1381 gatagtttag tcaaacagga atgaacttcg gagaacctga cgcgaaagtg ttcagtactt
     1441 ccagttagac ccgaagtatg gagaaccaca tcatagcgac cgtagtatac ttcgggctga
     1501 caggactgga ggctggactt aaagttcttc ttattggaat tgtgataaca caatccataa
     1561 caagatgaac tcatggtctc ctcaagcctg ctggtcaggc tcaaagctcc aacctcagac
     1621 accactggtc taaccttttt gagtttccgc ctgtccacaa atggatggca gacctcagca
     1681 atgatgtctt ggttcttcac aatctcgcac accgatggtc gcatagacgg atacttccgc
     1741 tgcggtctga tgttctccgc ctgtcaaagg agagagattg ctacgttaaa gggaatggtc
     1801 tcaggccaag gtgatttaac actaggctta ttgcggatgg tttccaatcg gtccgctacc
     1861 ggaagcctga gacttaatga atgtgtgggt cggtggctgt attaagcccg gggggactta
     1921 gggtctctcc cctatagtga tagtttagtc cccccccttc ccctatagtg tgggtttcat
     1981 tcgttagcgg cctgctgctg ctccctcgcc tgtctgtcct gattctcaat accttgacgt
     2041 ggaccttcag gcatactctg gaacatagcc tcaatttctt tcggtctcgt gattcggtca
     2101 tcgacaataa tgttcagcag cttaaagaga ccgtgtgcca actgcgggtc atcatcaaga
     2161 ctaatctctc cgggatggac cgcctgattg ccgacaatac gacagatatc tgccgcctgc
     2221 tggatgcgta caggtagtcc tttctggacg agtgaccgga tgtctttatt aatgttttct
     2281 cccggctcgc ctaagtggac cattagcttc tgaaggcaaa gacgaaggag tgcagcggcc
     2341 cctttaggag acagattaat gatgcttcgg gcttccatat agtcggactt gcagttatct
     2401 ggcatatctg gatttggtag ttcaacattg ccagccgccg gaataatcat attgccgtca
     2461 taccaataag catcatcacc gcaatgagag catgttgaca ttcggaactc ggttgtcctt
     2521 tgcgccaact cacgcgcact tccatacatc ctacgccaga actgacgggc atatacacca
     2581 cagtgaggac agttaaaagc ctctgcttca aacaccgccg gataatactc agccatttaa
     2641 tccagtctcc ttataaacac ctgccatcac attaaccaat ctatccacct cgacctcttt
     2701 agcgtaacgg tcgtaagtta tagaccctga gctatgaccc ataatcgcct gagcatacgc
     2761 taactcgacc ccatgttgtt ttaactgggt cgcaacagtg tgcctgaatg agtgaaaggc
     2821 taagccgcta cggtcagcaa ggactttagg cagcaacgtt ctgttaaacc atttgctcac
     2881 ctgctcaccg tagccattct tcatcagctt gaggccatcg aataccatag cattatcacc
     2941 cgcagtcttt ctacggtctt ccacaagcga cataaagtca gccaatacaa aaccataagc
     3001 gccatcgacc agcgggacac agcggtcgct atgagcgttc ttaatacttt tacccggtag
     3061 gctgctatcg tcttcgttga tatggatata tacggtcccg gcctcagtag ccctgacgtc
     3121 tttgacctga agctgggcca cctcgttaag tctcgcacct gtaatcgcag ccagagcggt
     3181 gacgtaataa tggtatggct tacctgcggt cttctgagag tacgctttag cggcgaccag
     3241 tagttgccct acctgctcag gactgaaagc atcacgagcg tcggaggctt tacgctgcgg
     3301 gactttcagc tcaagacctt cggtcaggtt cttagctatc aggtcgttac gtaccgccca
     3361 cttaaagaca gcagccatct tgataagata tttattgttg atggtaacga catcaagaca
     3421 gtcagtctta gactcccggt tcagcaggtc agacagcggc atagacttaa agcgttgctt
     3481 gcggttcttc ggtagctgtt ggaggacttc acggacccta agcatatctg cacgggtcgc
     3541 cttgccgaca tcctgaatat ccagatagtc gaatatctcg attagcgtag agtgtgacgc
     3601 tcggttttcg cttagggtcg ctggcttcca gttctgggcg ttctcctgct cgtactgctc
     3661 aatgagagac gcgagggtca gtgctttagg ttcagggact tcaggctccg atgatacaga
     3721 tagggagacg gacggtctta gactgccagt ctcaggctca aggtaagaca acaggccaga
     3781 gttatcgccc ttgagtctat cctgacaggc tttaagtaca ccgataacgt cattgatata
     3841 gcgatgctgg tctacagtca gacgctcagt cgctacagct tcccgaaggt tggacttgat
     3901 gtcgtcgtac tggtcaacgt aaagctggta ggactccggg tcgttcaggt catcacggct
     3961 gacaccaagt tcccactcaa caatggtctt cagatgctcc cgtagttcct catatgatgc
     4021 gctagggttg tctgcgtgta ttgctttaag tgctgacttg atgtgccgtg agtgagccat
     4081 agcggtacgt ctgtccttag tccgtagtga tactgacgct agtttatacc ggttgtgaga
     4141 aggaagcgaa gcggtcaggc gaccagtggt cttcatcctg agacggatgt aaaagacacc
     4201 gttacgctga taaaggtagg ttcttgaatt gagcatgtac tgagacctta ggtctgctca
     4261 ctacttcaac aaagttagtc acagtgttgg ttacagtgag taaaactatc ggaaggcttt
     4321 aagccgtatc ccttaggatt cagtaagtta gtagacttta agaatggtca tggagcgggc
     4381 gaagggaatc gaaccctcgt atagagcttg ggaagctctt gttctaccat tgaactacgc
     4441 ccgcttcgag atgcgtaagg cattataaac cttacgctct ccttagcaag tgccgcgctg
     4501 ctgactgctg attaattcgc catcagcatt ttggcttgct gccctgcggc ggcaagtaac
     4561 gtagcggatc aattgccgtt gcacggtaac gaatctggaa atgcaggcga acagatgccg
     4621 catccgtgct ccccatagtg gcgatttttt gcccagcctt cacgctttgc ccattattta
     4681 ccagcatcgt gtcattatgg gcgtaagccg taatgtaatc ttcactgtgt ttaatcatga
     4741 tgagattacc gtagccacgc agctggttgc ccacatacac cacctttcct gcacccgcgg
     4801 cgtaaatagg tgtaccccgt ggagctgaga tatcaatccc tttattgccg ccatctgctg
     4861 tcgaatacgg catgataact ttccctgtcg ttggccataa ccaacaacgt tgccctactg
     4921 gcggccagga agatttcggt accgctgatg acggtgtaac cgatgcggtt ttggtcgttg
     4981 atttggcggt tgatttacgt gtactactgc tacttttcgc cccacccagt tttagtttct
     5041 gaccaacttc aatggtgtaa gggggggaaa tgccgttcag tcgcgccagt tcttttacgc
     5101 tggttcccgt ggtgcgcgaa atacgatata gcgtatcccc ccgtttcacg gtgtaaacgg
     5161 agccggaata cgttcctgta tcggatgatt tgctacccga acagcccgcc aaaagcagtc
     5221 caaccgataa caacatcacg atacccagag attttttatt caggcgtccc gcactcaaaa
     5281 tctttcctcg cttaaaaaac aagacgcctt atcatagcag ccagcctctt gatacccaat
     5341 ctcttgcttc tggaaaaacg tggtaataga gaaagaggtt aatttcattt ggcaaaggga
     5401 agtatcatca gcaaaatatt cgctgtagag gtatcgaact tcctgacttt ttaccctatt
     5461 tcccatcctg atgcgtaata atcccaattt atcacattga ttttatcgcc actcatattg
     5521 atacgtatca ctgcccctat tcagagtcct gtttcgtatc atatctgtgt ttttttaacg
     5581 tcatcacact tcctacctcc cctaacgctt atcgtcgttt ctggcgtaaa tcttgcctgc
     5641 ttagactaaa tctttgccat aagaaccaaa tgttcaaatc gaggggattt ctatggaagc
     5701 gcgggaagca accgctacgg gtgaatcatg catgcgcgtc aatgccattg ctaaggtcac
     5761 cgggcgggca cgatatactg acgattatgt tatggcgggc atgtgttatg cgaaatatgt
     5821 acgtagccct atcgcacatg gttatgccgt aagtattaat gatgaacaag ccagaagttt
     5881 gccgggcgta ctggcgattt ttacctggga agatgtgcct gatattccat ttgctactgc
     5941 tgggcatgcc tggacacttg acgaaaacaa gcgcgatacc gccgatcgcg cactgctaac
     6001 tcgccatgtt cgtcatcatg gtgacgccgt tgccatcgtc gtggcccgcg atgaactcac
     6061 ggcagaaaaa gcggcgcaat tggtcagcat tgagtggcaa gaattacccg ttatcaccac
     6121 gccagaagcg gcgctggcag aagacgctgc accaatccat aacggtggca atttactgaa
     6181 acaaagcacg atgtcgacgg gtaatgtcca acaaacaatc gatgccgccg actaccaggt
     6241 acaggggcac tatcagaccc ccgttattca acattgtcac atggaaagcg taacatcgct
     6301 ggcgtggatg gaggatgact cgcgaattac catcgtttcc agcacccaga tcccgcacat
     6361 tgttcgccgc gtggttggtc aggcgctgga tattccctgg tcatgcgtac gagtcatcaa
     6421 accgtttgtc ggtggcggtt ttggtaataa acaggatgta ctggaagagc caatggcggc
     6481 attcctgacc agcaagcttg gcggcattcc ggtgaaagtt tcccttagcc gtgaagagtg
     6541 tttcctcgca acccgtaccc gccacgcttt taccattgac gggcaaatgg gcgtgaaccg
     6601 cgacggaaca ttgaaaggtt atagtctgga tgttctgtct aacaccggcg cttatgcatc
     6661 tcacgggcac tccatcgctt ctgcgggggg gaataaagtc gcttaccttt atcctcgttg
     6721 tgcctacgct tacagttcaa agacctgcta taccaacctc ccctcggctg gtgcgatgcg
     6781 tggttatggc gcgccacaag tcgtatttgc cgttgagtct atgcttgatg acgccgcgac
     6841 agcgttaggt attgatcctg ttgaaattcg tttacgcaac gccgcccgcg aaggagatgc
     6901 taatccgctc acgggcaaac gtatttacag cgcagggttg ccggagtgtc ttgaaaaagg
     6961 ccggaaaatc tttgaatggg aaaaacgccg tgcagaatgc cagaaccagc aaggcaattt
     7021 gcgccgcggc gttggcgtcg cctgttttag ctacacctct aacacctggc ctgtcggcgt
     7081 agaaatagca ggcgcgcgcc ttctgatgaa tcaggatgga accatcaacg tgcaaagcgg
     7141 cgcgacggaa atcggtcagg gtgccgacac cgtcttctcg caaatggtgg cagaaaccgt
     7201 gggggttccg gtcagcgacg ttcgcgttat ttcaactcaa gataccgacg ttacgccgtt
     7261 cgatcccggc gcatttgcct cacgccagag ctatgttgcc gcgcctgcgc tgcgcagtgc
     7321 ggcactatta ttaaaagaga aaatcatcgc tcacgccgca gtcatgctac atcagtcagc
     7381 gatgaatctg accctgataa aaggccatat cgtgctggtt gaacgaccgg aagagccgtt
     7441 aatgtcgtta aaagatttgg cgatggacgc tttctaccac cctgaacgcg gcgggcagct
     7501 ctctgctgaa agctccatca aaaccaccac taacccaccg gcgtttggct gtacctttgt
     7561 tgatctgacg gtcgatattg cgctgtgcaa agtcaccatc aaccgcatcc tcaacgttca
     7621 tgattcaggg catattctta atccactgct ggcagaaggt caggtacacg gcggaatggg
     7681 aatgggcatt ggctgggcgc tatttgaaga gatgatcatc gatgctaaaa gcggcgtggt
     7741 ccgtaacccc aatctgctgg attacaaaat gccgaccatg ccggatctgc cacaactgga
     7801 aagcgcgttc gtcgaaatca atgagccgca atccgcatac ggacataagt cactgggtga
     7861 gccaccaata attcctgttg ccgctgctat tcgtaacgcg gtgaagatgg ctaccggtgt
     7921 tgcaatcaat acactgccgc tgacgccaaa acggttatat gaagagttcc atctggcagg
     7981 attgatttga ggataacatc atgtttgatt ttgcttctta ccatcgcgca gcaacccttg
     8041 ccgatgccat caacctgctg gctgacaacc cgcaggccaa actgctcgcc ggtggcactg
     8101 acgtactgat tcagctccac catcacaatg accgttatcg ccatattgtt gatattcata
     8161 atctggcgga gctgcgggga attacgctgg cggaagatgg ctcgctacgt atcggctctg
     8221 caacgacatt tacccagcta atagaagatc ctataactca acgtcatctc ccggcgttat
     8281 gtgctgcggc cacgtccatt gctggaccgc agatccgtaa cgtcgctacc tacggtggaa
     8341 atatttgcaa cggtgccacc agcgcagatt ctgccacgcc aacgctaatt tatgacgcga
     8401 aactggagat ccactccccg cgcggtgttc gtttcgtccc gattaatggc tttcacaccg
     8461 ggccgggcaa agtgtctctt gagcatgacg aaatcctcgt cgcctttcat tttccgccac
     8521 agccgaaaga acacgcgggc agcgcgcatt ttaaatatgc catgcgcgac gcaatggata
     8581 tttcaacgat tggctgcgcc gcacattgcc gactggataa cggcaatttc agcgaattac
     8641 gcctggcatt tggtgttgcc gcgccaacgc cgattcgctg ccaacatgcc gaacagactg
     8701 cacaaaatgc gccattaaac ctgcaaacgc tggaagctat cagcgaatct gtcctgcaag
     8761 atgtcgcccc gcgttcttca tggcgggcca gtaaagagtt tcgtctgcat ctcatccaga
     8821 cgatgaccaa aaaagtgatt agcgaagccg tcgccgcggc ggggggaaaa ttgcaatgaa
     8881 tcacagcgaa acaattacca tcgaatgcac cattaacggg atgccttttc agcttcacgc
     8941 cgcaccaggc acgccgctct cggaattact ccgcgaacaa ggactgctaa gtgtcaaaca
     9001 agggtgctgc gtgggtgaat gtggtgcctg tacggtgttg gtcgacggca cagcaataga
     9061 cagttgctta taccttgccg cctgggctga aggaaaagag atccgcacgc tggaaggtga
     9121 agcgaaaggc ggaaaacttt ctcatgttca gcaggcttat gcgaaatccg gcgcagtgca
     9181 gtgcgggttt tgtacgcctg gcctgattat ggctaccacg gcaatgctgg cgaaaccacg
     9241 cgagaagcca ttaaccatta cggaaattcg tcgcggactg gcgggaaatc tttgtcgctg
     9301 cacggggtat cagatgattg taaatacagt tctggattgc gagaaaacga agtaaaagga
     9361 tatccggcct gaattcaggc cggattcact gaggttatgt gtttaacaac tcatatttct
     9421 taatcttgcg atagagcgta gcaatgccga tgcccagttc atcagcaact tgcttcttgc
     9481 tgttatgacg tgaaagcgcc tcgcggatca tttgcttttc catctcctcc agcgccgtgc
     9541 cgcccgcatc atcgagtgac aggtgcgcct cactgacctc tgttacatca ctttgctccg
     9601 ttgtgccatt attcagcaga tttggcggca atagcgtgct gtcgataact tcacctgaag
     9661 gaactacgtt aaccagatat tccatcaaat tgcttaactc gcgcaggttt ccgggccaac
     9721 gatgcttacg caatatttcg acgacatcgg gagcaatgcc aggataaacc gatcccagac
     9781 gacgggtatg cagatgtaaa aagtaatgca ccaatagttc aatatcttcc tgacgttcac
     9841 gcagcggtgg cagagttatc gggataacat taagtcggta gaagagatct tcgcggaatt
     9901 taccttcggc aatgaactgg gccaaattct gattagttgc agaaatgatg cgaatgtcga
     9961 cttgtattgg gctactggca ccaatcggta gaatttcacg cgcctcaata gcgcgcagta
    10021 atttagcctg caacattaat ggcatatcac ctatttcatc gagaaacagc gtgcccgtat
    10081 tcgccgcctg aatcaaccct gttttaccgt tggcagaagc gccagtaaat gcgcctttaa
    10141 cataaccgaa cagttcgctt tccagaagct gctccggaat cgcggcacag ttgatagcaa
    10201 taaagggttt attccgtctt ccgctcaact tatggattgc tcgggcgacg acttctttac
    10261 ccgtgccgct ttcaccaacc accataacgc tggatgggct gggtgcaata cggctaatga
    10321 gtcgttttaa ttgccgcata acacggcact cgccaaccaa ttgttcaata tgtggttcat
    10381 caggtgcatt tgctacagaa aaactggtat gcgattggtg aaatgccatt aaaaataatt
    10441 gtcggccctg aatgttatgc aattgaccaa tgattaattc acttttatcg tcccacgaaa
    10501 caatatgctg catatgtcca tgagtaaaat tactctcaaa tgttaatggt ctgaaacgga
    10561 taggtttccc aataatatta ttttgtacaa caccaagtgt ttttaaggca gtctgattaa
    10621 caaactgaac ccgactttca tcatcaacaa ctaatacgcc ctgatccata ttatcgatca
    10681 tggtcgcgaa tattttactg atgttatctc ctggcccctg atcctccaga agtttcgaaa
    10741 caaaaatggt ggatatatgg cgaacataat cagaaaattc gcgtaaatta tcactgatat
    10801 gctcttgttg ctcgtgggta acggcaatca aacttatcac cccaacacaa cgatcctgta
    10861 aaatgacagg cgtacccaga aatgcttttt cacggcaatt ttctttacta tcgcaacctt
    10921 cgcaaagggg atcgaagcga gactgtgtca caactttttc agttttcgtt tccaggacgt
    10981 ggcggagcag gcgtgagttg ccgctcaact ggcgaccaag aaacttccca tacgcgcccg
    11041 ttccggcgac gcgacacaag ttttcatcaa cgatctcaac ctcaagctgc aaaacgctgg
    11101 caagcattct ggcaaaacgc tgaattgtcg gttgaatttg catcaatact gactgcgtag
    11161 tagcaagctc catagcttta ccttccagac ttacttaaaa gtcgatcatt gaagacgttg
    11221 atggttcaca gatcatgatg atattaactc aggcgaaatt ggctttgata aaaacataag
    11281 atttttatca ttttctaatg aaattatgga agagatatca catttctata tcaatatgag
    11341 aattacggcg gtgagtttat caaactgaag agagatagcc tgtcccttta tcttatttct
    11401 gatacttagc agcaaataaa taacgcgata aaaaaagcca aacgttttcg tattttacaa
    11461 acaaccggaa gctggcatca atttgtgatc aaccccacac attatccgtc aaattagtct
    11521 tttgcagccc cgtggataat tctggcacac ttattgttag tcccaggtat agctgtgaaa
    11581 acaccaatca ctttggcaag tcacagtgaa ataaaccact ttgcctgtca ttccactacc
    11641 gggactttat gatgaaaact gttaatgagc tgattaagga tatcaattcg ctgacctctc
    11701 accttcacga gaaagatttt ttgttaacgt gggaacagac gccagatgaa ctgaaacaag
    11761 tactggacgt tgccgcagca ttaaaagcac tgcgtgctga aaacatctca accaaagtct
    11821 ttaatagtgg attaggtatt tccgtattcc gcgacaactc cacccgtacc cgcttctctt
    11881 atgcttccgc gcttaacctg ctcggccttg cacaacaaga tctcgacgaa ggcaaatcac
    11941 aaatcgctca cggcgaaacc gtgcgtgaaa ccgccaatat gatctccttc tgcgccgacg
    12001 ctattggtat tcgcgacgat atgtatctgg gcgcaggcaa cgcctatatg cgtgaagttg
    12061 gcgctgcact tgatgacggt tacaagcagg gtgtactgcc acagcgtccg gctttagtga
    12121 acctgcaatg cgatattgac cacccgactc agtcaatggc tgacctcgcg tggttacgtg
    12181 aacactttgg ttcactggaa aacctgaaag gtaaaaaaat cgccatgacc tgggcctact
    12241 ctccaagcta tggcaaaccg ctctctgtac cacaaggcat catcggtctg atgactcgct
    12301 tcggtatgga tgtcaccctg gcccatccgg aaggctacga cctgatcccg gatgtggttg
    12361 aagtggcgaa aaacaatgct aaagcatctg gcggtagctt ccgtcaggtc accagcatgg
    12421 aagaagcgtt caaagatgca gacatcgttt atccgaagtc atgggcacct tacaaagtga
    12481 tggaacagcg taccgaattg ctgcgcgcta acgatcacga aggcttaaaa gcactggaaa
    12541 aacagtgtct ggcacagaac gcgcaacaca aagactggca ttgtactgaa gagatgatgg
    12601 aactgacccg tgatggcgaa gccctgtaca tgcactgcct gccagctgat atcagcggcg
    12661 tatcctgcaa agaaggggaa gtgactgaag gcgtattcga aaaataccgt atcgctacct
    12721 acaaagaagc cagctggaag ccttatatca tcgccgcgat gatcctgtcc cgtaaatacg
    12781 ccaaaccagg tgcactgctc gagcaactgc tgaaagaagc gcaagaacgc gtgaaataac
    12841 ccctcgggcc gatcagctgg tcggccctct atttccagag gccaaaagga taggatatgt
    12901 ccgttttctc attgaagatt gatatcgccg ataacaaatt tttcaacggc gaaacatcac
    12961 cgctcttttc gcaaagccag gccaaactgg cgcgccagtt ccaccagaaa atagctggtt
    13021 atcgcccaac accgctttgt gcgctggacg atctcgcaaa cctttttggg gtgaagaaaa
    13081 ttcttgtcaa agacgaatca aaacgattcg gtctgaacgc cttcaaaatg ctcggcggtg
    13141 cgtacgctat cgctcaatta ttgtgtgaaa aatatcatct tgatatcgaa acgctgtcat
    13201 ttgagcacct gaaaaatgcc atcggcgaaa aaatgacttt cgcgaccacc accgacggca
    13261 accacgggcg cggtgtggcg tgggcagcgc agcaactcgg acagaatgcg gtgatttaca
    13321 tgccgaaagg ttctgctcag gaacgcgttg acgccattct gaacctcggt gccgagtgca
    13381 tcgtcacaga tatgaactat gacgataccg ttcgcctgac catgcaacac gcgcagcagc
    13441 acggctggga agtggtacag gacacggcat gggaaggtta caccaaaatc ccaacctgga
    13501 tcatgcaagg ctacgcaacc ctggcagatg aagccgtcga gcaaatgcgt gaaatgggcg
    13561 taaccccgac gcacgttctg ctgcaagccg gtgtcggagc aatggccggt ggtgtgctgg
    13621 gttatctggt cgacgtctat agcccgcaaa atctgcacag cattattgtt gaacctgaca
    13681 aagctgactg tatttatcgc tccggcgtca aaggcgacat cgtcaacgtt ggcggtgata
    13741 tggccaccat catggcaggc ctggcctgtg gcgaacctaa cccgctgggc tgggaaatcc
    13801 tacgtaactg cgccacccaa ttcatctcct gccaggacag cgttgccgca ttaggtatgc
    13861 gcgtgctggg taatccgtac ggcaacgacc cgcgcatcat ctccggtgaa tccggcgctg
    13921 tcggtttggg cgttctcgca gcggttcatt atcacccgca acgtcaaagc ctgatggaaa
    13981 aactggcgct gaacaaagat gccgtggtgc tggttatcag cactgaaggc gacaccgacg
    14041 tgaagcacta ccgcgaagtt gtctgggaag gcaaacacgc tgtagcacct taatcactct
    14101 attggaacta ctccctgcaa aacggggagt aaaaaatctg gagaaaaata atggctaaga
    14161 atattccatt caaactgatt cttgaaaaag caaaagatta ccaggcggac atgactcgct
    14221 tcctgcgcga catggttgct attcccagtg aaagctgcga cgaaaaacgc gtagtacatc
    14281 gtattaaaga agagatggaa aaagtcggct tcgataaagt tgaaatcgac ccgatgggca
    14341 acgttctcgg ttatatcggc cacggcccgc gtctggtggc aatggacgct catatcgata
    14401 ccgtcggcat tggcaacatc aaaaactggg acttcgatcc gtacgaaggc atggaaactg
    14461 atgagctaat cggtggtcgc ggtacttccg accaggaagg cggcatggca tctatggttt
    14521 atgccggtaa aatcattaaa gacctcggtc tggaagatga atataccctg ctggttaccg
    14581 gtactgtgca ggaagaagac tgcgacggtc tgtgctggca gtacattatt gaacaatccg
    14641 gcattcgccc ggaatttgtg gtcagtaccg aaccaaccga ctgccagata taccgtggtc
    14701 agcgcggtcg tatggaaatt cgtattgatg ttcagggtgt tagctgccac ggttctgcgc
    14761 cagaacgcgg tgacaacgcc attttcaaaa tgggtccgat tcttggcgaa ttacaagaac
    14821 tctcccaacg tctgggttat gacgaattcc tcggcaaagg caccctcacc gtttctgaaa
    14881 tcttcttcac atccccaagc cgttgcgctg tagcagatag ctgcgccgtc tctattgacc
    14941 gccgtctgac ctggggcgaa acctgggaag gcgcgctgga cgaaatccgc gccctgcctg
    15001 cagtacagaa agctaacgcg gttgtttcta tgtacaacta cgaccgtccg tcctggactg
    15061 gcctggttta cccaaccgaa tgctacttcc cgacctggaa agtggaagaa gatcacttca
    15121 ccgttaaagc actggtgaat gcctacgaag ggctgtttgg caaagcgccg gttgttgata
    15181 agtggacctt ctcaactaac ggcgtatcta tcatgggccg tcacggcatt ccggtgatcg
    15241 gctttggccc gggtaaagaa cctgaagcgc atgcacctaa cgaaaaaacc tggaaatctc
    15301 acctggtgac ctgtgccgcg atgtacgctg caatcccgtt aagctggctg gcaacagagt
    15361 aattactctt cacttattcc cctccggttc gccggagggt ttttggagtt tgctatgcgc
    15421 gtattgatca aaaacggcac tgtcgttaac gcagatggac aagccaaaca ggatttgctg
    15481 attgaaagcg ggattgttcg ccagttgggc aacaatattt cgccgcagct cccgtatgaa
    15541 gaaattgatg ccactggctg ttacgttttc cctggcggcg tggatgtcca tacgcatttc
    15601 aatattgatg tcggcatcgc gcgcagttgt gatgattttt ttaccggtac ccgcgcagct
    15661 gcgtgtggcg gtacaacaac cattattgac catatgggat ttggcccaaa cggctgtcgg
    15721 ttacgccatc aactggaggt ttatcgtggt tatgccgccc ataaagcggt catcgattac
    15781 agctttcacg gtgtgatcca gcacattaat cacgcaatcc tcgacgaaat cccgatgatg
    15841 gtcgaggaag gactgagcag ttttaaactc tatttaacct atcaatacaa actcaacgat
    15901 gacgaggttt tgcaggcatt acgccgtctg catgaatccg gcgcgctgac caccgtgcac
    15961 ccggaaaatg atgcggctat cgccagcaag cgggcggaat ttatcgccgc agggttaacc
    16021 gcgccgcgct atcatgcctt gagtcgccct ctggaatgcg aagcggaagc catcgcccgc
    16081 atgattaacc tggcacaaat tgccggtaac gccccgctct atatcgtgca cctgtctaac
    16141 ggcttaggtc tggattatct gcgtcttgcc cgtgcgaatc accagccagt ctgggttgaa
    16201 acctgcccac aatatctcct gttggacgaa cgcagttacg atacagaaga cggcatgaag
    16261 ttcattctta gcccaccgct gcgtaacgta cgcgagcagg acaaactgtg gtgtggcatc
    16321 agcgatggtg cgattgacgt ggtggcgacc gatcactgca ccttctcgat ggctcaacgc
    16381 ctgcaaattt ctaaaggcga tttcagtcgc tgcccaaatg gcttacccgg tgtggaaaac
    16441 cgcatgcagt tactgttttc cagtggcgtg atgacgggac gtataacacc ggaacgcttt
    16501 gttgaattaa ccagcgcaat gcccgccagg ctgtttggcc tgtggccgca aaaaggatta
    16561 ttagcgcccg gttccgatgg cgacgtggtg attatcgacc cacgtcagag ccaacaaatt
    16621 cagcatcgcc atctccacga caacgccgac tactcgccat gggagggttt tacctgtcag
    16681 ggcgcgattg tcagaacctt atcccgtggt gaaacgattt tctgtgacgg cacctttaca
    16741 ggcaaagccg ggcgaggtcg tttcctgcga cgcaaaccgt ttgtccctcc cgtgctctaa
    16801 ccactgtcag ggatctatac acaatcaaga atgatgagtg aggaaaaatg agtaagaaaa
    16861 ttgttctcgc cctgggcggg aatgcgctgg gcgatgacct ggccggacaa atgaaagcgg
    16921 taaaaattac ttctcaggca attgttgatt taattgccca aggacatgaa gttatcgtca
    16981 ctcatggtaa cgggccgcag gtgggcatga ttaaccaggc ctttgaagct gctgcaaaaa
    17041 ctgaagcgca ctcgccaatg ctgccgatgt ctgtctgcgt tgccttaagc cagggttata
    17101 ttggttacga tctacaaaac gccttaagag aagaactgct ttctcggggc attaataaac
    17161 cggtagcaac gctggttact caggtcgaag tcgacgctaa cgatccggca ttcctcaacc
    17221 cgaccaagcc gatcggttcg ttctttaccg agcaagaagc agagcaactg acaaaacagg
    17281 gttacactct gaaagaagat gctggtcgcg gctatcgccg tgttgttgcc tcgccaaaac
    17341 cggttgatat tattgaaaaa gaaacggtta aagctctgct agatgcaggc caggtggtga
    17401 tcaccgttgg cggtggtggt attcctgtta ttcgtgaagg taaccatctg cgtggtgcca
    17461 gcgcggttat cgataaagac tgggccagcg cccgtttagc agaaatgatt gatgccgata
    17521 tgctgatcat tctgactgcg gtagaaaaag tggccattaa cttcggtaaa gagaatgaac
    17581 agtggctcga tcgcctgtca ttaagtgatg ctgaacgctt tatcgaagaa ggtcattttg
    17641 cgaaaggttc tatgctgcct aaagtggaag ctgccgcctc gtttgctcgc tctcgcgcag
    17701 gtcgtgaagc actgattacc gtattgagca aagcgaaaga ggggattgaa ggcaaaaccg
    17761 gaacggtgat ttgtcagtaa ttttaaggcc gggtgggtaa taccatccgg ccattttcat
    17821 ttaagccact tccagcactt cttttgtcgc tttcacgccc tgatgcatca acatcattaa
    17881 cgcctcaagt acgccgccgc caatcgcccg ggctttatca gaaacgctgg tgaaatcagc
    17941 cgtttcacca cgaggatcga tatcaccaat tttaaaacca ccgaccactg ccaggccgtc
    18001 gttcaacaag ccacgcacca tccccgtcaa cggtgcttta atttcatgct caccaatcca
    18061 ggcaatcaca tcgccctctt tcaccagatc gcctaatttc acgttggatc gcataatgcc
    18121 tgcagcagga gcacggatca cccgtcgggt ggtatgcccc ataatattgc caggaacacc
    18181 ggtattctcc tgcgcacaac cagagtaaat cacctgaccg agccagtgcc cgcgatttgt
    18241 ttcaattacc gcatgacaat ccttccctgc agtaaagccc ggcccaagcg cgattgttac
    18301 tggtgccata tctgcccgcg ttcccaaatt ctgtttcgcc agaatagcgt ccaccacgca
    18361 aagcggtttc agttcatcaa gcaatgaaca ggcgggatct gccatcacag ggatgaatcc
    18421 gcgttcggta agtttcatcg cttccgcagt actggttgcc aggcgagcag taacgccttc
    18481 gaccgtcatt tcgccatcga acacggcctg ggcaaacgcc acggtacaac gaatcactgt
    18541 cggtttttcc acttccagca tgatcacttt aaaacccgca tgatacagac gtagcgccac
    18601 accactggcg atatctcccg caccgcgaat caccaccagc tggtgtccgg aaggatgaga
    18661 aggtttcatc atcagcccac ccggcgcgtt atttttcacc tgtaatattt ctgccagcac
    18721 gctgatggcg atctcctgcg gcgtttccgc acctatgttg taaccaacgg gcgcatgtaa
    18781 acgggcaata tgttcttcag ccacgccttt ctcacgcaat tgacgcagga aaagctgaac
    18841 cttgcggcga cttgccagca accccaacca tgcaatgggc tgttcaatga gtttgtcgag
    18901 ggcttcacga tcctgattat tcgtggcaat caggacaaaa ttatcagggc gaatatccag
    18961 tgcttccact gctgcaccaa acgactcagc atggagaagc gtggttgatg gagggaatag
    19021 ttcaggattg aggctttcgc gataaatatc ggcaacggca atatcaaatc ctaatagcgc
    19081 cgcactctgg gctatcgccc ggttgacatg ccccgcgcca atcaacacca gacgtggacg
    19141 cataccatgg acgctgataa atactgacat tgcacctcca caatctgacc cgacagcatc
    19201 cgcaccgtta cgagccatac gtccatggaa taatcgcggc ttacgttcct gcaatgcctg
    19261 aagtgactct tcaatcacct tccgctcaac cattccgcca ccaattgtac cgacgataga
    19321 accatcggcg cgcactaaca tttgtgcaga atggcgggga gtcgagcctc ggctatcaac
    19381 aatttgcgcc atcgcaaacg gacaattttg ctcttcgagt tttgcagcct ctgtgaaaat
    19441 attcatacca acctcaatgg atatcctttc agtaacccgg aatacccggg ccgctaattc
    19501 acaaatctgc gcgcgattgc aggatgttct tgtatatcgc ccagccagat tgcctctacg
    19561 tcgtgttgtt gcagcggttg aaggagctcg ctttgtgcaa tcgcattctc acattgagaa
    19621 aaacggttaa tgaaccagac tcgccgacaa ccttgcggta cgtttttaaa cgccccctga
    19681 gggtggcgaa ccagcgcaac gagatcgctc agttgcaagg ttgcatcagg tgttaacccg
    19741 gtaatatcag caaactgcga ccagcgatgg acattttctg tgctcacttt cgcgcccaaa
    19801 gtatgtcctc ccatcacagc aatcacgcag caactgcttt tgggtatgca aggttcgtgc
    19861 tcatcaggcg cttttaacgg cattccacgc gagccatctg cttcaatgag aattacgtca
    19921 cactctggtc gttgcaccag tgcatcaatc gcttctggcg taaatccctg cacttttccc
    19981 tggttcgctt tccagctgtg aaaacaaaat gaaatggggg atgtaagaga cgcatgagga
    20041 agcatggcgg gatcacgaca gaaaaccacg ggccagtgag acgttggcat aaacatatgt
    20101 gtcgtcgtcg tgattaacac acgcctgccg ctagcctgga gcagttctgc cagccaaaaa
    20161 agcaagctgg ttttcccccc agcaccaaca acagaaatca cagtgggacg tttctgcgca
    20221 cctaaatcaa tgactaatgc cgatgggtca actatacttt tcacgagtct ttatgacctc
    20281 tctgggataa attatcccca acttaatcca tcaggaagta acgcaattat caggcgttat
    20341 tagcccctat aaataatgga accactatgt cagccatcga ctgtataatt accgctgccg
    20401 gattatcatc aagaatgggg caatggaaaa tgatgttacc ctggcaacag gggacaattc
    20461 ttgatacaag tatcaaaaat gcgttgcagt tttgtagccg aattatttta gtcaccggct
    20521 atcgtggtaa tgaactgcac gaacgttatg cgaaccagag caatatcact attatccaca
    20581 acccagatta tgcgcagggt ttactaacat cagtaaaggc cgcagtaccc gcagtgcaaa
    20641 cagaacattg ttttctcacc cacggtgaca tgccaaccct caccatcgat atttttagaa
    20701 aaatctggtc gttacgaaat gatggcgcaa tactgccact ccataatggc atccccggcc
    20761 atccgatttt agtgtcaaaa ccatgcttga tgcaggcaat ccaacgaccc aatgtcacca
    20821 atatgcgtca ggcactcctt atgggagctc attattccgt cgagatagaa aatgcagaaa
    20881 taattttaga tattgatact ccggacgact ttattacagc gcaaaaaagg tatactaaaa
    20941 tttagaaaat gtagttaaac gattgcgttc aaatatttaa tctctccagc actctttatc
    21001 ataatgataa ggaatcactg attttgagaa aagagaaaaa gtgataaatg ggctgacaat
    21061 aataattttc aggagtgatt ttaattctca tttggcacct gtgagttcat tcacaaaaag
    21121 cctttaagtt tcgagccgcc tcacattttt ttatatttcc cgccaaacct ggcaagagtg
    21181 gtgcgattgt tgctctatcc ccctaaacca ccggatttct caacaccggt cactcaatga
    21241 tatctgtata agctaaggag agggttatgg gggatattat gcgtcccatt ccgtttgagg
    21301 aacttttgac gcgcatattt gatgaatacc aacaacaacg ctcaatcttt ggtattcccg
    21361 agcaacagtt ttactcacct gtaaaaggta aaactgttag cgtcttcggt gaaacctgtg
    21421 ccactcccgt cggccctgcc gctggcccgc acacgcagct cgcgcaaaat attgtcactt
    21481 cctggctgac tggcggacgc ttcatcgaac taaaaaccgt ccaaattctt gaccgcctgg
    21541 agctggaaaa gccctgtatc gatgccgaag acgagtgctt taacaccgaa tggtctaccg
    21601 aatttaccct gcttaaagcc tgggatgaat acctcaaagc ctggtttgcc ctgcaccttc
    21661 tcgaagcgat gttccagcct tctgattccg gtaaatcgtt catctttaat atgagcgtcg
    21721 gttacaacct cgaaggtatt aagcaaccgc caatgcaaca gttcatcgac aatatgatgg
    21781 acgcatctga ccatccgaaa ttcgcgcaat atcgcgatac gctgaataaa ttactccagg
    21841 atgacgcatt tttagcccgc cacggattgc aggaaaaacg cgaaagcttg caagccttac
    21901 ccgctcgcat ccccaccagt atggtgcagg gcgtcaccct ctccaccatg cacggctgtc
    21961 ctccgcatga aatcgaagcc atttgccgct acatgctgga agaaaaaggg ctcaacacct
    22021 ttgtgaaact taacccgacc ttactgggat acgcgcgtgt tcgtgagatc ctcgatgtct
    22081 gcggtttcgg ttacataggc ttaaaagaag agtcatttga tcacgacctc aagctgacgc
    22141 aagcactgga aatgctggaa cgcctgatgg cactggcaaa agaaaaatca ctcggctttg
    22201 gcgtaaaact gactaacact ctcggcacca tcaacaataa aggcgcactg cctggtgaag
    22261 agatgtatat gtcaggccgt gcgctgttcc cgctctccat caatgttgca gcagttctct
    22321 ctcgcgcctt tgacggcaaa ctgcccattt cttattccgg tggtgccagt cagctgacta
    22381 tccgcgatat ttttgataca ggtattcgcc ctattactat ggcaaccgac ctgctgaaac
    22441 ctggcggcta tttgcgctta agtgcctgca tgcgcgagct ggaaggctcc gacgcctggg
    22501 gacttgacca tgttgacgtc gaacgactga acagactggc agcagatgcg ttaaccatgg
    22561 aatacaccca gaaacactgg aagccagaag agcgtattga agtggcagaa gacctgccgc
    22621 tgaccgattg ctacgttgcc ccctgtgtta ctgcctgcgc tatcaagcaa gatattccgg
    22681 aatacatccg tctgcttggc gaacaccgct atgccgacgc gctggaactc atctaccaac
    22741 gcaacgctct gcccgccatt accggtcata tttgcgatca ccagtgccaa tacaactgta
    22801 cccgcctgga ttacgacagt gcgctgaata tccgcgaact gaaaaaagtc gcgctggaaa
    22861 aaggttggga tgaatataag caacgctggc acaaaccagc cggttctggt tcacgccatc
    22921 cggttgccgt gattggtgca ggtccggcgg gtctggcagc aggttacttc cttgccagag
    22981 cgggccatcc ggttacgctg tttgaacgcg aagccaatgc gggcggcgtg gtgaaaaata
    23041 tcattcctca gttccgaatt ccggctgagt taattcagca cgatatcgat tttgttgccg
    23101 atcacggcgt gaaatttgag tatggctgct cacccgattt gaccgttgaa cagttaaaaa
    23161 atcagggctt ccactatgtt ctgattgcca ccggcactga taaaaatagc ggtgtgaaac
    23221 tggcgggtga caaccaaaat gtttggaaat cactcccctt cctgcgtgaa tacaacaagg
    23281 gtacagcgct caagctgggc aaacatgtgg tcgttgtcgg ggcgggtaac accgcaatgg
    23341 actgcgctcg tgcggcgtta cgcgttccag gcgtagaaaa agcaacgatc gtttaccgtc
    23401 gttcactgca agagatgccc gcatggcgcg aagagtatga agaagcgttg cacgacggcg
    23461 tggagttccg tttcctgaat aatccggaac gtttcgatgc tgatggcacc ttaaccttgc
    23521 gcgttatgtc gcttggcgaa cccgatgaga aaggccgtcg ccgtccggtt gaaaccaacg
    23581 aaacagtaac actgcatgta gacagcctga tcaccgccat tggtgaacag caggatactg
    23641 aagccctgaa tgcgatgggc gtgccgctgg acaaaaacgg ctggccagac gtcgaccaca
    23701 atggcgaaac tcgtctgact gacgtcttta tgatcggcga cgtacagcgc ggaccatcct
    23761 ccattgtcgc tgctgtcgga accgcgcgtc gggcgaccga tgccatcctt agtcgggaaa
    23821 atatccgttc ccaccagaac gataaatact ggaacaacgt caatccggcg gaaatctatc
    23881 aacgtaaagg cgatatctct atcactctgg tgaacagtga cgatcgtgac gcgtttgtcg
    23941 cccaggaagc cgctcgctgc ctcgaatgta actacgtttg cagcaagtgt gtggatgtct
    24001 gcccgaaccg cgccaacgtc tccattgcgg tcccaggctt ccagaataga ttccagacgc
    24061 tgcacctcga cgcttactgt aacgaatgcg gcaactgcgc tcagttctgt ccgtggaacg
    24121 gtaaaccata caaagacaaa atcaccgttt tcagcctggc gcaagacttt gataacagca
    24181 gcaacccagg cttccttgtg gaagattgcc gggtacgagt acgactgaat aaccaaagct
    24241 gggtgttaaa catcgacagc gaaggtcagt tcaacaacgt accaccggag ctgaacgata
    24301 tgtgccgcat catcagccat gtccaccagc atcatcatta tctgctgggc cgcgtggagg
    24361 tgtaatcatg ttgattctga agaatgtcac cgcagtgcag ttacacccgg cgaaagtgca
    24421 ggaaggcgtt gatatcgcta tcgaaaatga tgtgattgtc gctatcggcg atgccctgac
    24481 gcaacgctac cccgatgcca gctacaaaga gatgcatggt cggattgtga tgccgggaat
    24541 tgtctgctcg cacaaccatt tttactcggg gctttcccgc ggaattatgg caaacatcgc
    24601 cccctgcccg gatttcatct caacgctgaa aaatctctgg tggcggctcg atcgcgccct
    24661 tgatgaagag tcgctctatt acagcgggct gatttgttcc ctggaagcga ttaagagcgg
    24721 atgtacatcg gttatcgatc accatgcctc tccggcgtat atcggcgggt cgctctccac
    24781 attgcgcgac gcatttttaa aagttggcct gcgcgcgatg acctgttttg aaactactga
    24841 ccgtaacaac ggcatcaaag agttgcagga aggtgtagaa gaaaacatcc gcttcgcccg
    24901 tcagattgat gaggcgaaga aagcagcaac cgagccgtat ctggtggaag cacatatcgg
    24961 tgctcacgcg ccgtttaccg tgccagatgc cggtctggag atgctacgtg aagccgtgaa
    25021 agccacaggc cgtggtttgc atattcacgc tgcggaagac ctttacgacg tttcctacag
    25081 tcaccactgg tacggcaaag acctgctggc acgactagcg caattcgatc tcatcgatag
    25141 caaaacgctg gtcgctcatg ggctgtactt gtcgaaagat gacatcgccc tactcaatca
    25201 gcgcgatgcg ttcctggtgc ataacgcccg ttcaaacatg aacaaccatg tcggctacaa
    25261 ccatcacctt agcgacatcc gcaatctggc gttgggaacg gacggcattg gttcggacat
    25321 gtttgaagag atgaaatttg ccttctttaa acatcgcgat gcgggtggtc cgctgtggcc
    25381 tgacagtttt gccaaagccc tgactaacgg taacgaactg atgagccgca actttggcgc
    25441 gaaatttgga cttctggaag ccggttacaa agctgattta accatttgcg attacaactc
    25501 accgacgccg ctgctggcag acaatatcgc cgggcatatc gctttcggta tgggctcagg
    25561 cagcgttcac agcgtgatgg tcaatggtgt gatggtctat gaagaccgtc agtttaactt
    25621 cgattgcgat tccatttatg cacaagccag aaaagccgct gccagtatgt ggcgtcggat
    25681 ggatgcgctg gcataaatga cagatgccct cttcccgcag ggaagagggt taaataagga
    25741 aagaagatga ttgaacaatt tttcaggccc gactctgtcg aacaggcgct ggaactgaag
    25801 cgccgctacc aggatgaagc cgtctggttc gccgggggca gcaaactcaa tgctacacca
    25861 acccgtaccg ataaaaagat tgccatttcc ttacaggatc tggaactgga ctgggttgac
    25921 tgggataacg gtgcactgcg gattggcgca atgtctcgct tgcagccact gcgtgatgcg
    25981 cgatttattc ctgcagcgct gcgtgaagcc ctcggttttg tttactcacg ccatgttcgt
    26041 aatcagtcga ccattggtgg tgaaatcgcc gcccgccagg aagagtcggt gctgcttccc
    26101 gtcctgttgg cactggatgc tgaactggtt tttggcaacg gcgaaacgct gtcaatcgag
    26161 gactacctgg cctgcccatg cgatcgcctg ctaaccgaaa ttatcattaa agatccgtat
    26221 cgcacctgtg cgacccgcaa aattagccgt tctcaggcag gtttaaccgt cgtgacggca
    26281 gccgttgcaa tgacagacca cgacggtatg cgaattgcgc tggatggcgt cgccagtaaa
    26341 gcactgcgtc tgcatgatgt cgaaaaacaa aatctggaag gcaatgcact tgaacaggct
    26401 gtcgccaacg ccattttccc gcaggaagat ttgcggggca gcgtggccta taaacgctat
    26461 atcacgggag ttctggtagc cgacctgtat gccgactgcc aacaggctgg ggaggaagcc
    26521 gtatgatcat ccactttact ttaaatggcg cgcctcagga gctaaccgtt aatccaggcg
    26581 aaaacgtgca aaagctgttg tttaacatgg gaatgcactc tgtacgcaac agtgatgatg
    26641 gcttcgggtt tgccggttct gacgcaataa tctttaacgg taatatcgtt aacgcgtcct
    26701 tgcttattgc cgcacagtta gagaaggcag atattcgtac cgcagaatct ttgggcaaat
    26761 ggaacgagtt aagtctggtt caacaggcaa tggttgatgt tggcgtggtg cagtctggtt
    26821 ataacgatcc agctgcagct ctgattatca ccgatcttct cgatcgcatc gccgcaccta
    26881 cccgcgaaga gatcgacgac gcgctttctg gtttgttcag ccgcgatgca ggctggcaac
    26941 aatactatca ggtcattgaa ctggcggttg cacgtaaaaa taatccgcag gccaccattg
    27001 atatcgctcc gactttccgt gacgaccttg aagtcattgg caagcattat cctaaaactg
    27061 atgccgcgaa aatggtgcag gcgaaaccct gctatgttga agaccgcgta acggctgacg
    27121 cctgcgtcat taaaatgtta cgtagcccac acgctcacgc actgattact catctggatg
    27181 tcagcaaagc tgaagcctta ccgggcgtcg ttcacgttat tactcacctg aattgcccgg
    27241 atatctacta taccccgggt ggtcagagcg caccggaacc gtcaccgctt gaccgccgta
    27301 tgttcggcaa gaaaatgcgt cacgtcggcg atcgtgttgc tgcggtcgtc gcagaaagtg
    27361 aagaaattgc gctcgaagca ttgaagctca tcgacgttga atatgaagtg cttaagccgg
    27421 taatgtcgat cgacgaagca atggcggaag atgcgcctgt cgtgcacgat gaaccggtgg
    27481 tgtatgttgc tggtgcgcca gatactctgg aagacgataa cagccatgca gcccagcgcg
    27541 gcgagcatat gatcatcaac ttcccgatcg gttctcgccc tcgcaaaaat atcgccgcca
    27601 gtattcatgg tcatattggc gatatggaca aaggctttgc cgatgccgat gtgatcattg
    27661 agcgaaccta taactcaacg caggcacagc agtgcccgac tgaaacacat atctgcttta
    27721 cccggatgga cggcgatcgt ctggttatcc acgcctccac ccaggtacca tggcacttac
    27781 gccgccaggt cgcgcgcctc gtaggcatga aacagcataa agttcatgtc attaaagagc
    27841 gagttggcgg cggttttggc tccaaacagg acatcctgct ggaagaagtg tgcgcatggg
    27901 caacctgcgt gaccgggcgt ccggtactgt tccgctacac ccgtgaagaa gagtttattg
    27961 ctaacacctc tcgtcacgtc gcgaaagtca ccgtcaaact gggagcgaaa aaagatggtc
    28021 gcctgacggc agtgaagatg gatttccgcg ccaacactgg cccttacggc aaccactcac
    28081 tcaccgtacc gtgtaacgga ccggcgctgt cgctgccgtt atatccgtgc gataacgtcg
    28141 atttccaggt caccacctac tacagcaaca tttgcccaaa tggtgcttat cagggttatg
    28201 gcgcaccgaa aggtaacttc gctatcacca tggcattagc ggaactggct gaacagttac
    28261 agatcgacca actggaaatt atcgaacgta accgggtaca cgaagggcaa gagctgaaaa
    28321 ttctcggtgc aatcggtgaa ggtaaagcgc cgacctccgt tccttccgcc gccagctgcg
    28381 cactggaaga gatcctgcgt cagggtcgcg agatgatcca atggtcttca ccgaaaccgc
    28441 aaaatagtga ctggcacatc ggtcgcggcg tcgccattat catgcagaaa tcgggtatcc
    28501 cggatatcga tcaggctaac tgcatgatca aactggaatc ggacggcacc tttatcgttc
    28561 attctggcgg tgcggatatt ggtactggtc tggatactgt agtgacgaaa ctggcagcag
    28621 aagtgctgca ctgcccaccg caggacgtgc atgttatctc cggtgatacc gatcatgcgt
    28681 tgtttgataa aggcgcatat gcctcgtccg gtacttgctt ctcgggtaac gcggcgcgtt
    28741 tggcagcgga aaatctgcgg gagaaaattc tgttccacgg cgcgcaaatg ttgggtgagc
    28801 cagtggcaga tgttcaacta gcaacgccgg gcgtcgtgcg cggcaagaaa ggcgaagtta
    28861 gtttcgggga tattgcccat aaaggcgaaa ccggcaccgg ctttggttca ctggtgggaa
    28921 ctggcagtta tatcacgcct gatttcgctt tcccgtatgg cgcaaacttc gctgaagttg
    28981 ccgtcaacac gcgtacgggt gaaatccgcc tggataaatt ctacgccttg ctggactgcg
    29041 gtacaccggt caatccagag ttagcgttgg gacaaatcta cggtgccacc ctgcgtgcta
    29101 tcggccacag tatgagcgaa gagatcattt ataacgccga aggtcacccg ttaacgcgtg
    29161 atttacgcag ttacggcgca ccgaaaattg gtgacattcc gcgtgatttc cgcgctgtgt
    29221 tggtgccgag cgacgataaa gtcggcccgt tcggggcgaa atcgatctcg gaaattggtg
    29281 taaatggcgc agctccggcg attgctaccg caattcacga tgcctgcggc atctggttac
    29341 gcgaatggca tttcacaccg gagaaaatac tcaccgcgct ggaaaaaata taagtaataa
    29401 atggccgctt cggcggcctg tttctctgtt tgaacgatga ctaaatagtg actgcgaaaa
    29461 tgagataagc cgttctcaag gcgtcctgcg ccccaaattc gtgggaattt attttactaa
    29521 ttcagatgat caaatttact ttaaaaggag tgaagggatg tctgatataa accatgcagg
    29581 ttctgacctt atatttgaac tggaggatcg ccctcccttt catcaggctc tcgttggtgc
    29641 cattacccat ctgttggcaa ttttcgttcc gatggtaacc cccgcgttaa tcgtgggtgc
    29701 ggccttacag ctttccgctg aaacaaccgc ctatcttgtt tctatggcga tgatcgcctc
    29761 tggtattggt acctggttac aagtaaaccg ctacggcatc gtcggttctg gcctactctc
    29821 aattcagtca gtcaattttt catttgttac ggtcatgatt gcgctgggca gcagcatgaa
    29881 aagcgacggt tttcacgaag agttaatcat gtcgtcgctt ctcggcgtct ccttcgttgg
    29941 cgcatttctg gttgtcggat cttcatttat cttgccctat ttacgtcggg ttattacgcc
    30001 taccgtcagc ggtattgtgg tactgatgat cggcttaagc ctgattaaag tcggcattat
    30061 cgattttggt ggaggatttg cagccaaaag cagcggtacg ttcggcaatt acgaacatct
    30121 cggcgttggt ttattggttt taattgtggt gatcggcttt aactgctgtc gcagtccgtt
    30181 gctacgcatg ggagggatcg ccattgggct atgtgtcggc tatatcgcat cgttatgcct
    30241 gggcatggtg gatttcagca gtatgcgcaa tttgccgtta atcaccatcc cgcatccgtt
    30301 caaatacggc tttagtttta gcttccatca gttcctggtg gttggcacga tttatctgct
    30361 tagcgtgctg gaagcagtcg gcgatatcac cgccacggca atggtttccc gccgccccat
    30421 tcagggggaa gagtatcagt cccgactgaa aggcggcgtg ctggcagatg gtctggtttc
    30481 tgttatcgcc tccgctgtcg ggtcattacc cttaaccacg tttgcgcaaa ataatggggt
    30541 tattcagatg actggcgtcg cttcacgtta tgtcgggcga accatcgcgg taatgctggt
    30601 tatcctcggt ttgtttccga tgattggcgg cttcttcacg accattccct cggcagttct
    30661 gggaggcgca atgacgttga tgttttccat gattgccatc gctgggattc gcataatcat
    30721 caccaacggt ttaaagcgcc gagaaacact tattgtcgcc acttctttag gtttaggact
    30781 tggcgtctcc tacgatcccg aaatttttaa aatattgcca gcctctattt atgtactagt
    30841 tgaaaaccct atttgtgctg gcgggttaac tgcgatttta ttaaatatta tcctccctgg
    30901 tggctaccga caggaaaacg ttctgcctgg tattacctca gcggaagaga tggattaaca
    30961 gtaaaggagt caatgatgtc aggagaacac acgttgaaag cggtacgagg cagttttatt
    31021 gatgtcaccc gtacggtcga taacccggaa gagattgcct ctgcgctgcg gtttattgag
    31081 gatggtttat tactcattaa acagggaaaa gtggaatggt ttggcgaatg ggaagacgga
    31141 aagcatcaaa ttcctgacac tattcgcgtg cgcgactatc gcggcaaact gatagtaccg
    31201 ggctttgtcg atacacatat ccattatccg caaagtgaaa tggtgggggc ctatggtgaa
    31261 caattgctgg agtggttgaa taaacacacc ttccctactg aacgtcgtta tgaggattta
    31321 gagtacgccc gcgaaatgtc ggcattcttc atcaagcagc ttttacgtaa cggaaccacc
    31381 acggcgctgg tgtttggcac tgttcatccg caatctgttg atgcgctgtt tgaagccgcc
    31441 agtcatatca atatgcgtat gattgccggt aaggtgatga tggaccgcaa cgcaccggat
    31501 tatctgctcg acactgccga aagcagctat caccaaagca aagaactgat tgaacgctgg
    31561 cacaaaaatg gtcgtctgct atatgcgatt acgccacgct tcgccccgac ctcatctcct
    31621 gaacagatgg cgatggcgca acgcctgaaa gaagaatatc cggatacgtg ggtacatacc
    31681 catctctgtg aaaacaaaga tgaaattgcc tgggtgaaat cgctttatcc tgaccatgat
    31741 ggttatctgg atgtttacca tcagtacggc ctgaccggta aaaactgtgt ctttgctcac
    31801 tgcgtccatc tcgaagaaaa agagtgggct cgtctcagcg aaaccaaatc cagcattgct
    31861 ttctgtccga cctccaacct ttacctcggc agcggcttat tcaacttgaa aaaagcatgg
    31921 cagaagaaag ttaaagtggg catgggaacg gatatcggtg ccggaaccac tttcaacatg
    31981 ctgcaaacgc tgaacgaagc ctacaaagta ttgcaattac aaggctatcg cctctcggca
    32041 tatgaagcgt tttacctggc cacgctcggc ggagcgaaat ctctgggcct tgacgatttg
    32101 attggcaact ttttacctgg caaagaggct gatttcgtgg tgatggaacc caccgccact
    32161 ccgctacagc agctgcgcta tgacaactct gtttctttag tcgacaaatt gttcgtgatg
    32221 atgacgttgg gcgatgaccg ttcgatctac cgcacctacg ttgatggtcg tctggtgtac
    32281 gaacgcaact aataataaaa ctttaacatc ctcgtgagga catcattatg tctggagaca
    32341 tcctacaaac accggacgca ccaaagccac agggcgcgct ggataattat tttaaaatta
    32401 ccgctcgtgg cagtaccgtt cgtcaggaag tactggctgg cttaacgacc tttctggcca
    32461 tggtttattc cgttatcgtc gttccgggaa tgctgggcaa agcaggtttt cctcccgcag
    32521 ctgtgtttgt tgccacctgt ctggtcgcgg gcttcggctc gttgctgatg gggttgtggg
    32581 ccaatttgcc aatggcgatt ggttgtgcga tttccttgac ggcatttacc gcattcagtc
    32641 tggtactcgg gcaacaaatt agcgttcctg tcgcactggg cgcggtcttt ctgatgggcg
    32701 tcatcttcac cgctatttcc gtaaccggtg tgcgtacctg gatcttacgt aatttaccga
    32761 tgggtatcgc tcacggtaca ggtatcggta tcggcctgtt tctgctgctg attgctgcta
    32821 acggtgtggg tatggttatc aaaaacccga ttgaaggctt gccagtggcg ctcggtgcgt
    32881 ttacctcctt cccggtgatg atgagcttgc tggggctggc ggtcatcttc ggtctggaga
    32941 agtgccgcgt acccggcggg atcttgttgg tgattatcgc aatttcgatt atcggcttaa
    33001 tctttgaccc agcggtgaaa taccacggtc tggtggcgat gccaagtctg actggcgaag
    33061 atggtaagtc tctgattttc agcctcgata ttatgggcgc actccagcca accgtacttc
    33121 cgagtgtact ggcattggtg atgaccgcag tgttcgacgc cactggcacc atccgtgccg
    33181 tcgccggtca ggcgaatttg ttggataaag acaaccagat catcaatggc ggcaaagccc
    33241 tgaccagtga ctcagtaagt tcaatattct ccggtctggt gggcgcagcg cccgcagcgg
    33301 tttatatcga atcagcggca ggaaccgccg ccgggggtaa aacagggttg accgcaaccg
    33361 tagtgggggc gttattcctg ctgattctgt tcttatcacc gctgtcattt ttgatccctg
    33421 gttacgccac tgcacccgct ctgatgtacg tgggtttgct gatgttaagt aacgtctcga
    33481 agctggattt caacgatttt attgacgcaa tggctggcct ggtgtgtgcc gtgttcatcg
    33541 ttctgacttg taatatcgtt accggtatta tgctgggctt tgtgacactg gtcgtaggcc
    33601 gtgtctttgc tcgcgaatgg caaaagttga atattggtac ggtgatcatt actgccgcac
    33661 tggtcgcatt ttacgcgggt ggttgggcaa tctaatagat tctccgcgcc tttcagcgcg
    33721 gagatttctt tcacagagga ttctcaccgc tggcaacaat acgctgctgg cggactcgcc
    33781 tgagttctct ttcagtaagc aactgcagcg cctgtgtcgg gcaagattca acacaggctg
    33841 gcccctcttc ccgctgttca cagagatcgc atttcacaat ttgttgtcgg gtatcccccg
    33901 gcaacgactg aatggtgatc attccaaacg ggcacgcact gacgcaactc tgacagccaa
    33961 tacaacgggc agaattggtc tgcaccacct gctcgcccat cgtcagcgcc cccacggggc
    34021 aagcgccaac acaaggggcg ttttcacact gatggcacat caccggcgca ctgatgctgt
    34081 ccagtcgctg taccttcagc cggggtaaaa agacatcggc attcaactcc tgttctgaag
    34141 ggtgagcgac cacacaggct acttcacaag tacggcagcc aatacagtca gccggattaa
    34201 cgataattaa cgatttcatc acgatgcctt cgtatcaaac agagttaaca tatcgcgcgc
    34261 cgcctgcctt cctgcggcca ttgcagtgac aaccagatcc gcgccatgaa ctgcatcacc
    34321 accagcaaag acttttttca gatgcgtctg ggtaggtaaa tacccgacgt caccggtttg
    34381 aatcaggccc catttatcga gtttaattcc gctgccctgc aaccacggca tggcatgcgc
    34441 ctggaaacca aaggccataa tcagaacatc ggcgggcaat tcaaactctg aacccgctac
    34501 cggacgagga cgacggcgac catccggccc cggctcaccc atggcggtac gaatcaggcc
    34561 caccgcagtt aaacgcccat cttcgtcaca agcgatatat tgcggttgaa cattgaactg
    34621 aaactcgaca ccttcctcgc gcgcattaac cacctctttg cgcgagcccg gcatactgac
    34681 ttcatcacga cgatacgcgc aggtcacgct ggcggcattg aggcggatgg aagtccgcaa
    34741 acaatccatt gtcgtatcgc caccgcccaa taccacgact cgcttacctt ccacgtccgt
    34801 cagcggatac tcttcagact ccggcaatcc catgagctgg cgggtatggg cagtcaggaa
    34861 cggtagagcc tgaatgacac cgggcgcatc ttcatgcggc agatctgctc gcatcatccc
    34921 gtaagtcccc acgccgatga aaactgcatc atattcagaa gttaaatcgc taaaggtaat
    34981 atcgcggcca atttcacagt taagatgaaa gtcgattccc attgcggtga atatctctcg
    35041 ccgctggctt aataccgttt tatcgagttt gaaaggagga atgccaaaag tcagcatacc
    35101 gccaatttct ggatggcgat caaagacatc aacctgaact cccgcgcgcg ccagaatatc
    35161 agcacaccct aaccctgctg gcccagcgcc aatcaccgcc actttttcgc tacggggaac
    35221 aactttgctg acatcgggac gccagcccat cgccagcgcg gtatcggtga tgtagcgttc
    35281 cagattaccg atagagactg cgcccgagtg atctttcaaa gtacatgcgc cttcacaaag
    35341 acggtcctgt ggacataccc tgccgcaaat ttctggtaag gaactggtct ggtggcaaag
    35401 ttctgccgct tcaataatct ttccttcctg taccagacgg atgtaatccg gaatagcgtt
    35461 atgcagcggg caatgccagt tgcagttagc tttttcggca caataaacac agcggtcact
    35521 ctcataagtc gcttgcagtg gatccagccc gcaatatatt tcgccaaagt gggttttccg
    35581 ttcactcgct gaaattttat ctgcgccttt acgcgagtta acggggagca acgctgcact
    35641 gcgagatggc tgagcatctg atgacgcttt tcctgctgcc gttttgcgct ggcgagccac
    35701 ctttatccac tgtaaccctt tatcgtccat cagtcgtaac gcctgtgttg ggcagacttc
    35761 aatacaggct tgcgtgccgg aactgcgctg gttacaaagg tcgcatttct gcgcaatcgt
    35821 atcgaccatc tcaacgacgc caaaggggca agcgattgcg catcttttac agccaataca
    35881 tttttgctcg tccagttgta cgctatcgga ctggaaagtc agagcattaa ccggacaagc
    35941 cgtaacgcaa ggggcattgt tgcagtgatg gcaggccacc ggattcgcag cctggccttt
    36001 ccctacaacg tggatacgcg gtcgaaagtc actgtgactc agcggccagt tttcttgatt
    36061 gtgtgccacc gcacaggcaa tttcacaagc atgacagcct atacattccg cagcttcagc
    36121 agcgataaac ttattcattt gcatcccttt catttaatga gttatgtctt ctcaaatttc
    36181 gcgatgcaat aaacgggcat atattaggta gcatgaccgt tttaattata attttgcgac
    36241 tgagttcaaa ttattgcccc tcaatctatg ccgctttcat tatttcaaca ttgccgacca
    36301 aaagagcgct atccagataa aaacgattaa atgcgagagt gcgacatgcc agaatgatta
    36361 atgaaatatc aatatgagaa aattacagat aatttcattg tcaggttctc ttctcatttt
    36421 cgtggtttaa ccacgattat gtgattgcct ccgcaattcc tgtctctaac tccccttcct
    36481 cgcaaaaact ggcactccac gagcatgtgt ttagacagtt tcattaacgt aaacggttgc
    36541 tttttactct ggcgggcgaa aggagaaaca ctgatgagcg ccatagattc ccaacttccc
    36601 tcatcttctg ggcaagaccg cccaactgat gaggttgacc gcatattatc accaggaaag
    36661 ctgatcatac tcggtctgca acacgtcctt gtcatgtacg caggtgcagt cgctgttcct
    36721 cttatgattg gtgaccgact gggcctctca aaagaagcta ttgcgatgct cattagctcg
    36781 gatctctttt gctgcgggat cgtcacatta ttgcaatgta tcggtatcgg ccgctttatg
    36841 gggatccgcc tgccggtgat tatgtcggtg acctttgctg ctgtaacacc aatgatagcc
    36901 attgggatga acccggatat cggcctgctg gggatatttg gtgccactat agccgcgggt
    36961 tttatcacca cattattagc gccacttatt ggtcgcttga tgcctttatt cccgccactg
    37021 gttaccggtg tggttattac ttctattggg cttagcatca ttcaggtggg tattgactgg
    37081 gccgccggag gtaaagggaa tccgcaatat ggtaatcccg tttatttagg tatctccttt
    37141 gccgtcttaa tttttatctt gctcattact cgctatgcga aaggatttat gtccaacgtc
    37201 gccgtattac tggggattgt atttggcttt ttactttcgt ggatgatgaa tgaagtcaat
    37261 ttatccgggc tacatgatgc ctcgtggttt gcgattgtca ccccgatgtc gtttggtatg
    37321 ccgattttcg atcccgtttc cattctgacc atgactgccg tgttaatcat cgtgtttatc
    37381 gagtcgatgg ggatgttcct ggcactgggt gaaatagtcg gtcgtaaact ctcttcacac
    37441 gatattattc gcgggctgcg tgtcgatggc gtagggacaa tgataggcgg cacgtttaac
    37501 agcttccccc acacgtcatt ttctcaaaac gttggcctgg ttagcgtgac gcgcgttcat
    37561 agccgctggg tgtgtatttc ttcgggaatt atattaatcc tgtttggcat ggtgccaaaa
    37621 atggcggtgc tggtagcctc cattccgcaa tttgtgctgg gcggcgctgg gctggtgatg
    37681 ttcggcatgg tactggcgac agggattcga attctgtcgc gctgtaacta caccaccaac
    37741 cgttacaacc tctatattgt ggcgatcagt ctcggcgttg gcatgactcc gacgctctct
    37801 cacgatttct tttctaagtt accggccgta ctgcaaccgt tgctgcatag cggcattatg
    37861 ctcgcaaccc ttagcgccgt tgtgctgaat gtcttcttta atggctatca gcatcatgct
    37921 gacctggtga aggaatccgt ctctgataaa gatttaaaag tcaggacagt acgtatgtgg
    37981 cttctgatgc gcaagctgaa gaaaaatgag catggagaat aatatgaatt ttttaatgcg
    38041 cgctatattc agtctgttgt tgctttttac tctctctatt cctgtcattt ctgactgtgt
    38101 tgcaatggcc attgaaagtc gcttcaaata tatgatgcta cttttttaaa tggtttttac
    38161 ctgtcggcat ccgctcaaaa cgggcggttg tcgataaacg ctcacttggt taatcatttc
    38221 actcttcaat tatctataat gatgagtgat cagaattaca tgtgagaaat tatgcaaacg
    38281 gaacacgtca ttttattgaa tgcacaggga gttcccacgg gtacgctgga aaagtatgcc
    38341 gcacacacgg cagacacccg cttacatcgc gcgttctcca gttggctgtt taatgccaaa
    38401 ggacaattat tagttacccg ccgcgcactg agcaaaaaag cgtggcctgg cgtgtggact
    38461 aactcggttt gtgggcaccc acaactggga gaaagcaacg aagacgcggt gatccgccgt
    38521 tgccgttatg ggcttggcgt ggaaattacg cctcctgaat ctatctatcc tgactttcgc
    38581 tatcgcgcca ccgatccgaa tggcattgtg gaaaatgaag tgtgtccggt atttgccgca
    38641 cgcaccacca gtgcgttaca gatcaacgat gatgaagtga tggattatca atggtgtgat
    38701 ttagcagatg tattacacgg tattgatgcc acaccgtggg cgttcagtcc gtggatggtg
    38761 atgcaggcag ccaatagtga agcaagaaaa ttgttgtctg ctttcgcgca gcacaattaa
    38821 aaaaccccga catttgccgg ggttgtgagc ataacgtaat gcttatttta ccggacgcat
    38881 cgccgggaac agaataacgt cgcggatggt atggctgttg gtgaacagca ttaccatacg
    38941 gtcgatgcca atccccagac ctgctgtcgg cggtaagcca tgttccagtg cggtaacgta
    39001 atcttcatcg tagaacatcg cttcgtcgtc acctgcgtct ttcgcggcaa cctgatccag
    39061 gaagcgttgc gcctgatctt ccgcgtcatt cagctcgcta aagccgttac cgatttcacg
    39121 accaccgatg aagaactcaa agcggtctgt gatttccggg ttaatgtcgt tacgacgcgc
    39181 cagcggagaa acttctgccg gatattcagt aatgaaggtc ggctgaatca gatgtgcttc
    39241 tgccacttct tcgaagatct cggtaacgat acggcccaga ccccagctct tctcaacgtg
    39301 gataccgata gattcagcaa tcgctttcgc agagtcgaag ttatccagat ccgccatgtc
    39361 ggtttccggg cgatatttct tgatcgcttc acgcatggtc agtttttcga acggtttacc
    39421 gaagtccagc gtcacgtcgc cgtaggtcac ttccgtctta ccgagaatat cctgtgccag
    39481 agtacggaac agcgattcgg tcagctcgat cagatctttg taatctgcgt aagccatgta
    39541 gagttccatc atggtgaact ctgggttatg acgtacggaa ataccttcgt tacggaagtt
    39601 acggttgatt tcgaatacac gctcgaagcc accgaccacc agacgcttga ggtacagttc
    39661 cggcgcgata cgcaggtaca tgtcgagatc cagcgcgtta tggtgggtga taaacggacg
    39721 cgcagcggca ccgcctggga tcacctgcat catcggcgtt tcaacttcca taaagccgcg
    39781 gttcaccatg aactggcgaa taccagagag gatctgcgag cgtactttaa aggtgttacg
    39841 ggattcatcg ttagagatga gatccaaata acgctgacga tagcgcgctt cctgatcctg
    39901 caagccgtgg aatttatccg gcagcggacg cagtgctttg gtcagcagac gcagctcggt
    39961 gcagtggata gacagttcgc cggttttggt tttgaacagt ttacctttcg cgccgaggat
    40021 gtcgccgagg tcccattttt tgaactgctc gttgtaaacg ccttccggca gatcgtcacg
    40081 ggcaacgtac agctgaatgc ggccacccac atcctgcagg gtaacgaaag acgctttacc
    40141 cataatacgg cgggtcatca tgcggccagc aacggcgact tcgatgttca gcgcttccag
    40201 ttcttcgttc tctttaccgt cgaattctgc gtgcaattgg tcagaggtat gatcgcgacg
    40261 gaaatcgttc gggaaggcaa ccccctgctc gcgcaggttc gccagcttct cacgacgcgt
    40321 tttcagttca ttgttaagat cgactaccgc gtcagcgccc tgtgcgtgtt gttcagacat
    40381 gttggttcct cataaccctg ctttcaaact tgcttcgata aattgatcca ggctgccgtc
    40441 cagcacggcc tgcgtgttgc gggtttctac cccggtacgc agatctttaa tgcgggagtc
    40501 atcaaggaca taagaacgaa tctggctgcc ccagccgatg tcggatttgt tatcttccat
    40561 cgcctgtttc tcggcatttt tcttctgcat ctccagttca taaagcttcg ctttcatctg
    40621 cttcatggcc tggtctttgt tcttgtgctg ggaacggtcg ttctggcatt gggtcacgat
    40681 cccggttggg atgtgggtaa tacgcaccgc agattcggta cggttaacgt gctgaccgcc
    40741 cgcgccggac gcgcgataaa cgtcaatgcg cagatccgcc gggttgattt cgatatcaat
    40801 atcatcatca acttccgggt aaacaaacgc agagctgaac gatgtgtggc gacgaccgcc
    40861 ggagtcaaac gggcttttac gcaccaggcg gtgaacgcca gtttctgtac gcagccagcc
    40921 gtaagcgtaa tcgccggaga ttttgatcgt cacggattta atacccgcca cttcaccttc
    40981 cgactcttcg atgatttcag ttttgaaacc acgcgattca gcccagcgca gatacatacg
    41041 ctcaagcatg ctcgcccagt cctgtgcttc cgtaccgcca gaccccgcct gaatatcgag
    41101 gtagcagtcg gcgctgtcat attcgccaga gaacatacgg cggaactcaa gctgcgccag
    41161 tttttcttcc agggcgtcga gttcagcaac ggcttcgtta aaggtttctt cgtcgtcagc
    41221 ttctacagcc agttccagca gaccagaaac atcttccagc ccctgtttca tttggtcgag
    41281 ggtgtcgaca acggcttcga gggaggaacg ctctttaccc agcgcctgtg cgcgttcggg
    41341 ttcgttccag acatccggct gttccagctc ggcgtttact tcttccagac gctctttctt
    41401 ggcgtcgtag tcaaagatac cccctaagaa cgtcggagcg ttccgtgagg tcctgaatgc
    41461 gattatttac cggattaatt tcaaacatgg tctgatttct tttattgagc tagtcaaaat
    41521 gcggtgataa gagcgggatt gtacccaatc cacgctcttt tttatagaga agatgacgct
    41581 aaattggcca gatattgtcg atgataattt gcaggctgcg gttgccgcga aactcgttga
    41641 tatcgagctt ataagccagt tgcacttcac gcacgccgtt atccggccag agggcggtat
    41701 cgacattaaa agcaatacca tccagcagtg gaccgccgcc gaccggttcg accatcacct
    41761 tcaaatgacg ttcgcccacc agccgctgtt gcagcagacg gaaatgaccg tcaaacagcg
    41821 gctccgggaa catctgcccc cacgggccag catcgcgcag caactgcgcc acttccatgg
    41881 tcatttcggc cgggcttaac ggaccgtctg acaccacttc gccttgcaat agcgaagggt
    41941 ccagccactc tgtaaccagt tcgccaaacc gttgttgaaa gagttcgaat ttatcctctt
    42001 ccagcgacaa acccgccgcc atcgcatgac cgccaaactt cagtatcatg ccagggtaga
    42061 gtgtgtctaa tcgctccagt gcatcacgca tatgcagccc ctgaatggag cgacctgaac
    42121 ctttcagcgt accatcacct gctggcgcaa aggcgataac cggacggtga aaacgctctt
    42181 tgatgcgcga agccagaata ccgacaacgc cctgatgcca ttcggggtga tacattgcca
    42241 gcccgccggg tagcgtgtcg cgacttcgct ccagtttctc gcacagggtc agggcttcaa
    42301 cttgcattcc ttgttcgatc tcttttcgcg tctggtttag cgcatcgagt tcatttgcca
    42361 gcacgcgcgc ttcgccgatg ttgtcgcaca gcaacagcgc cacaccgacg gacatatcgt
    42421 ccagtcgccc ggcagcattg agacgtggcc ccagcgcaaa acctaaatcg ctggcggcga
    42481 gtttttgcgc atcgcgattt gccacttcca gcagcgcttt aatccccgga cggcactttc
    42541 cggcacggat gcgactcatc ccctgccagg tcagaatgcg attattagcg tccagcggca
    42601 cgacgtccgc cactgttccc agcgcgacca gatccagcag ttctgccagg ttaggaattg
    42661 cgatgccacg ctcatcaaac cagccctgat cgcgcaaaaa ggtgcgcagc gccagcatca
    42721 gataaaacgc cacacccacg cctgccagtg atttcgacgg gaaattacag tcgcgcaagt
    42781 tagggttaat gatcgcttcc gccgcgggta atgtttcgcc tggcaaatgg tgatcggtaa
    42841 caataaccgg aatgcccaac gagcgagcgt gtacaacccc cgcatgggag gaaataccgt
    42901 tatccaccgt gacaattaac tgcgcgccac gggcatgggc ctgatcgacc acttccgggc
    42961 ttaagccgta accgtcttcg aaacggtttg gtaccaggta gtcgatattg ctgcaaccaa
    43021 gcgagcgcat cgccagcacg cttagagccg tgctggtcgc gccgtcggcg tcgaaatcac
    43081 cgaccacaat aatccgcgtt ccttcgcgaa aagcgttgta aaggatctca acggcctttt
    43141 cgacgccgct cagttgctgc cagggcaaca tacctttaac actgcgttcc agttcttgcg
    43201 cactgcgcac gccccggctg gcgtataaac ggcgcagcaa gggaggcaat tcagcgggca
    43261 agtctgccgt ttcatcgact tcacggcgac gaagttgtat ctgttgtttc acgcgaatta
    43321 tttaccgctg gtcatttttt ggtgttcgtc gaggaattct ttcatctctt tcggcggctg
    43381 gtaacccgga acaagtgtgc cattgctcag cacaactgcc ggagtaccgc taacgccaag
    43441 ctggacgcca agtgcgtaat ggtcggcaat atccacgtcg cagctggctg gtgcgacgct
    43501 tttacctgcc atcacatcat caaacgcttt gtttttatct ttcgcacacc agatagcttt
    43561 catttctttc tctgcatcgc tgtccagccc ctggcgcggg aaagcaagat aacgcacggt
    43621 gatccccagc gcgttatagt ctgccatttg ctcatgcagt ttgtggcagt aaccacaggt
    43681 aatatcagta aacacggtga tgacgtgttt ttcctgcggc gctttataaa cgatcatctc
    43741 tttttcaagc gcattcaact gctttaacag catcttattg gtgacattga ccggagccgt
    43801 gccgctaacg tcatacattg gcccctgaat gatatgttta ccatcatcgg tgatgtacaa
    43861 cacgccgctg ttagtcagaa ctgtcttcat gccagctacg ggcgcgggct gaatatcgct
    43921 gcttttgatg cccattttgg ctaacgtttg ttgaattgcc gcgtcatcag cctgagcaaa
    43981 acctgaaaac gccgctaaca aagtaaacaa cataaaacct ttcttcataa atcttcccgt
    44041 tcttttcaga catcacgccc gcgggtgatg ctgttgatga agttgccgca gacgctcggt
    44101 agcgacatgc gtataaattt gcgtggtgga aagatcgctg tggcccagta gcatctgcac
    44161 cacgcgtaaa tccgcaccat gatttaataa atgagtggca aaagcgtgac gcaacacatg
    44221 cggtgacagc ttttcgctgt cgatacccgc cagcacagca taatgtttaa tgcggtgcca
    44281 gaaggtctgt cgcgtcatct gctgcgcacg ctggctggga aacaacacgt caattgacac
    44341 accattcagc agccacggac gcccatgttc cagataggtt tccagccagt aaaccgcctc
    44401 ttcacctaac ggtaccaggc gctctttgtt gcctttacca atgacccgca ctacgccctg
    44461 acgcaggctg atatcactca ttgtcagtcc gaccagttca gagacacgca gcccggtagc
    44521 atacaacact tcaagcatgg ctttatcgcg tagctccagt ggctgatcaa ttaatggtgc
    44581 ccgtaataaa cgttcgacct gcgcttcgct taaatctttt ggcaaacgct ggggcaattt
    44641 cggtgaagcg agatgcgcac tgggatcgtc ttcacgaaac ttttcgcgat aaagatactg
    44701 gaacaatcgg cgcactgcac tcagcaaacg cgctgagctg gtggctttat acccgccctc
    44761 cagccgttct gccagtaatg cctgcaaatc gtcactttgc gccgtcgcca gcgtcaaccc
    44821 gcggtgatgc aaccactcca ccatcattga cagatcgcga cggtaagcgt tcaacgtatt
    44881 ttcagccaga tttttttcca gccacagagc atcaagaaac tgctcgatgc gtgccagatc
    44941 ctgtttcact tgcgcccctt atggtcactc atttgatcca ttatgcctta ttgtgccgtg
    45001 actaaagcga ttctgataca ctagctgcaa aagccacagc agaatcgaga agcttacgtt
    45061 atgaatatgg gtctttttta cggttccagc acctgttaca ccgaaatggc ggcagaaaaa
    45121 atccgcgata ttatcggccc agaactggtg accttacata acctcaagga cgactccccg
    45181 aaattaatgg agcagtacga tgtgctcatt ctgggtatcc cgacctggga ttttggtgaa
    45241 atccaggaag actgggaagc cgtctgggat cagctcgacg acctgaacct tgaaggtaaa
    45301 attgttgcgc tgtatgggct tggcgatcaa ctgggatacg gcgagtggtt cctcgatgcg
    45361 ctcggtatgc tgcatgacaa actctcgacc aaaggcgtga agttcgtcgg ctactggcca
    45421 acggaaggat atgaatttac cagcccgaaa ccggtgattg ctgacgggca actgttcgtg
    45481 ggtctggcgc tggatgaaac taaccagtat gaccttagcg acgagcgtat tcagagctgg
    45541 tgcgagcaaa tcctcaacga aatggcagag cattacgcct gatctcactg acggcttagc
    45601 gcatatgctt tgccgtcatc tttatctttg cgtctcttgt tgcaacaaaa tccgccgtaa
    45661 atcccgccat tcagcttcgt ccatgctgtc ggctgccagc cataaatgtt gccgtttacc
    45721 gccatcagaa cgtaaacgca gcatcatgcc gctcttaatc atccacggtg ctttgacgat
    45781 gctccactcc tgcccttgcc aacgcaaacg cccgtccatc aacaagcgaa tttccccctg
    45841 gcgagcatta atacgccgct ggctgcgaac gcaatcaaac accaccagcg aaagtaacac
    45901 catccataac ggggtgtaac tgagtggcca gggcatgagt aaaataacag cggcaaccag
    45961 cccatgaatc agcaaggaaa gccactgtgc gcgccaggag acgcgcaaat cagattgcca
    46021 caggaccacg ttcccggttc cgtgtctgga tgagtcggac catcatttcc agttctgcat
    46081 cggccggttt accgtgattc atcagccagt taaacaggtc cggatcgtca cattccagca
    46141 gacgaataaa gatgcgtttt tcgtcatcgc ttaagctgtc gtactcatgt tcgaaaaacg
    46201 gcatgattga aatatcgagt tcgcgcatac cacggcggca tgcccaatga atgcgggctt
    46261 tgttgttaat gtccatcttc ttcctgtctc acgaaaatcc agtaccccgc tattgtaacg
    46321 tgtttttcgc cttcttttac gggaatatca gtaaacacca tcgcgatcgc gaaattaatt
    46381 cacaacaatt caatggcttc atttttttgg aagtcgcctc gcagaaggca cagatcgcgt
    46441 agtgaaagca cttgcattgc ctcatagctc ttttaccatt agtcattaat acgccgttaa
    46501 gcaactcagg actctattat ggcttttaca ccttttcctc cccgtcagcc tacggcttct
    46561 gcccgtttac cgctgacgct gatgacgctt gatgactggg cgcttgccac cattactggc
    46621 gcggacagcg aaaaatatat gcagggtcag gtgacagcag atgtcagcca gatgacagaa
    46681 aatcagcacc tgctcgccgc ccattgcgac gccaaaggta aaatgtggag caatttacgt
    46741 ctgttccgcg acggcgatgg ctttgcatgg attgaacggc gcagcgtgcg tgaaccgcag
    46801 ctgactgaac tgaaaaaata tgcggtattc tctaaagtga ccatcgcgcc agacgacgag
    46861 cgtgtgctgc ttggtgttgc cggttttcag gcgcgcgccg cgctggcaaa tctctttagc
    46921 gaactgcctt cgaaagaaaa acaggtagtc aaagaaggcg cgaccacttt gctatggttt
    46981 gaacacccgg cagaacgttt cctgatcgta accgatgaag ctactgctaa tatgctgacc
    47041 gataaactgc gcggtgaagc ggaactgaac aatagccaac agtggctggc attaaacatt
    47101 gaagcgggtt tcccggtgat tgatgccgcc aacagcgggc agtttatccc acaggcgacc
    47161 aatctccagg cgctgggcgg tatcagcttt aagaaaggct gttataccgg acaagagatg
    47221 gtggcgcgag caaaattccg tggtgccaat aaacgtgcgc tctggttgct ggcaggtagc
    47281 gccagccgac tgccggaagc tggtgaagac ttagagctga aaatgggcga gaactggcgt
    47341 cgtaccggta cggtgctggc tgcggtaaaa ctggaagatg gtcaggtcgt ggtacaggtc
    47401 gtcatgaata acgatatgga accggatagc atcttccgcg tacgcgacga tgcgaataca
    47461 ttgcatatcg agccgctgcc gtattcgctc gaagagtaaa tctctttatc gcatcaggca
    47521 tttatcgcct gatgcgacgc tgacgcgtct tatcatgtct gggatttgat gcctttatca
    47581 caacagggac gaggcccgta gatcggataa ggcgttcacg ccgaatccgg cagttgtgct
    47641 ccgatgcctg atgcgacgct ggcgcgtctt atcaggccta caaaggcata cccattacgc
    47701 ctgcccaata tacaaataga tcgccagaaa gtggcacaca ctaccgccga gcacgaagcc
    47761 gtgccagatg gcatggttgt atggaatgcg tttgcagacg tagaaaatca ccccgagcga
    47821 ataaaccacg ccgcctaccg ccagtaaggt aacgctgccc gccgcgagct taactgccat
    47881 ttcataaatt accaccagcg acagccagcc catcgccaga taggtcacca gagataaaat
    47941 tttgaatcgg tgcgcgatgg tcagtttaaa cagaataccc agcaatgcca ggctccagat
    48001 aacaatcatc aacccgcgcg ctaacggaga atccagcccc accagcaaaa acggcgtgta
    48061 ggttccggca atcaacaggt aaatagcgca atggtcaaat ttcttcagcc acatttttgc
    48121 ccgttgatga ggaatggcgt gatagagcgt cgaagcgagg aacagcagga tcatactgcc
    48181 gccatagagg ctgtaactgg ttatcgcagt ggcgctggca ttgagatcca ccgcctgaac
    48241 cagtagcaac accagcccaa cgataccaaa caccaaccca atgccgtgac tgacgctgtt
    48301 ggcaatttcc tctgccagcg aatatccctg cttaatgagg ggcttctgaa ccataactta
    48361 ctccggagaa acgtacacgc acatgtatac ctctccagcg taactgagaa tagttccagt
    48421 gaacacctgt tagctaaaat aaattctgat ttgagactaa tctcctaaaa atcatgaaat
    48481 taaatgcgaa atttcaacta acaggcgcga gttcaattta aagacattta aattcaatca
    48541 cataaaactg tgtctgaccg ggatagatgt cggcaatgac ctttatcagt tcagtcaggg
    48601 tcatattttc ctgctctgca tgtttttcag tcagcgtatc cagcgttacg gttgaggttg
    48661 cggtgacttc aatcgtgcaa aaataaccgt catcttcaaa acgtccgaca cgaagcacat
    48721 cacccgtttt gaagtgcgat tcagactcgt cgcggatggt gatggtttta cgcccagcca
    48781 gaatgtcatc ctggaaacgt tgaaaaaaag tgatgtcgtt tggctgcatg gtactatttc
    48841 ctgtaagaat tgactcatct ggagcctatg atagtgaaaa aactcacctt accgaaagat
    48901 ttcttatggg gcggcgcagt tgccgctcat caggtcgaag gcggctggaa caaaggcgga
    48961 aaagggccga gcatttgtga cgttctgacc ggtggcgcac acggcgtgcc gcgcgaaatc
    49021 accaaagaag tcttgccagg caaatactat ccaaaccatg aagccgttga tttttatggt
    49081 cactacaagg aagacatcaa gctatttgcc gaaatgggct tcaaatgttt tcggacttcc
    49141 atcgcctgga cgcgtatttt tccaaaaggt gatgaagctc agccaaacga agaagggctg
    49201 aagttctacg atgatatgtt cgatgaactg ctgaaataca acatcgaacc ggtgatcacc
    49261 ctctcccact ttgaaatgcc gctgcatctg gtgcagcaat acggtagctg gaccaaccgt
    49321 aaagtggttg atttctttgt ccgtttcgcg gaagtggtgt ttgaacgcta taagcataaa
    49381 gtcaaatact ggatgacctt caacgaaatt aacaaccagc gtaactggcg tgcaccgctg
    49441 ttcggttact gctgctccgg cgtggtttat accgagcatg agaacccgga agagacgatg
    49501 tatcaggtgc tgcatcacca gtttgtcgcc agcgccctgg cggtgaaagc tgcgcgtcgc
    49561 attcacccgg agatgaaagt cggctgtatg ctggcgatgg tgccgctcta tccctactgc
    49621 tgtaacccgg acgatgtgat gttcgctcag gagtcgatgc gcgaacgcta cgtctttacc
    49681 gatgtgcagc tacgcggcta ttacccgtcc tatgtgttga acgagtggga gcgtcgcgga
    49741 tttaacatca aaatggaaga cggcgatctc gatgtactgc gtgaaggcac ctgcgattat
    49801 cttggtttca gctattacat gaccaacgca gtgaaggccg aaggcggcac cggcgatgcg
    49861 atctctggtt tcgaaggcag cgtaccgaac ccgtatgtta aagcgtctga ctggggctgg
    49921 cagattgatc cagtaggtct gcgctatgcc ctttgcgaac tgtatgagcg ttatcagagg
    49981 ccgctgttta ttgtcgaaaa cggttttggc gcttacgaca aagtggaaga agatggcagc
    50041 atcaacgacg actaccgcat tgactacctg cgcgcccata tcgaagagat gaaaaaagcg
    50101 gtgacttacg atggcgtgga tctgatgggc tacacaccgt ggggctgcat cgactgcgta
    50161 tcgttcacca ccgggcagta cagcaaacgc tacggcttta tctatgtgaa taaacatgac
    50221 gacggtgctg gcgatatgtc gcgttcacgt aagaagagct ttaactggta ccaagaggtg
    50281 attgccagca acggcgagaa gctttaagtc gatgaagtac cggatgcaat acttgttgca
    50341 tccggtcaga caacttccct gttttatttc ccgcccgcca aatcgataaa acttcccgtg
    50401 acgtaagagg ctttatcact tagtagccag acaatggcct gcgcgacctc ttctgcctgt
    50461 ccaccacgct gcatggggat gttcgactta acgcgatcga cgcgtccagg ctcgccgccg
    50521 ctggcgtgca tttcggtata aataaaccct ggccgcacgc agttaacgcg gatcccctgc
    50581 gcggcgactt ccagcgatag tccggtggtt aacgtatcaa tcgccccttt cgatgccgtg
    50641 taatcaacat attcccctgg cgaacccaac cgtgaggcca ccgaagagac attgacgata
    50701 gcgccgccac tgccaccatt tttaagcgcc atgcgtttta ccgcctcgcg gcagcagaga
    50761 aaatatcccg tcacgttggt ggaaagtact cggttgattc gctctgcggt aaggttttca
    50821 acggtgcact gggtaaacaa gatcccggcg ttattgacca gcgctgctag cggttcatcg
    50881 tgctgatcga ttgctgtaaa catcgcaacg acctggtttt cgtcgctgat atccgcctgg
    50941 agcacgaatg ctttgccacc ggcttgcgtt attaagttca tcacttcctg cgccgcgtgg
    51001 aggttttgct gataattaac cgccaccgta tacccttctt gcgccaacag taatgcagtt
    51061 gcccgcccga tgccgcgact gccaccggtc acaagtgcta tagccatttg tttttccgag
    51121 aaaaattcat tcgttgacgt atctttgaga ttgctaacaa actggctcaa tcttcaatgt
    51181 tggcacgctt ttccccctcg ccctttcagg gagagggccg gggtgagggt aaatattcgc
    51241 gccagtgccg gcctgttccc ctcaccctct ccccaaaggg gcgaggggac tgtctgagca
    51301 cttttgtact ttgtcatctg actaaaaagg cgccgaagcg cctttagaaa atagtcgaat
    51361 cagtgaatta ctggtattcg ctaatcggta cgcaggagca gaacaggtta cggtcgccgt
    51421 aaacatcatc cagacgtttc accgtcggcc agtatttgtc tgccacacct gccgggaata
    51481 ctgcaacctc acggctgtac ggatgcgccc actcggcgac cagttcgttc tgaatgtgcg
    51541 gcgcgttcac cagcgggtta tcttccagcg gccagacacc ggctttaacc tggtcgattt
    51601 ctgcgcggat agccagcatc gcgtcgataa agcgatccag ttccactttg ctttcagatt
    51661 cagtcggttc aaccatcagc gtacccgcca ccgggaacga catggtcggc gcgtggaaac
    51721 cgtagtcgat caggcgcttg gcaatatcca gctcgctgat gccggtttct tctttcagcg
    51781 ggcgaatatc gagaatacat tcgtgcgcca cgcgaccgtc gcgaccggta tacagcaccg
    51841 ggaaggcatc ctgcaggcgg ctggcaatat agttggcgtt gaggattgcc acctggcttg
    51901 cttttttcag cccttctgcg cccatcatgc ggatgtacat ccagctgatt ggcaggatag
    51961 aggcgctacc gaacggtgcc gcagaaactg cgccctgacg ggttaacatg ccttcgattt
    52021 gcaccacgct atgacccggt acaaacggtg ccagatgcgc tttcacaccg atcggtccca
    52081 tacccggacc accaccgccg tgcggaatgc agaaagtttt atgcaggttg aggtgcgaaa
    52141 cgtccgcgcc aataaagccc ggcgaggtga tgccaacctg ggcgttcatg ttcgcgccat
    52201 caaggtaaac ctgaccgccg aactgatgca cgacttcaca cacttcacgg atcgtttctt
    52261 catacacgcc gtgggtagaa ggataagtca ccatgataca ggagaggtta tcgcccgcct
    52321 gttccgcttt cgcgcgcaga tcagtcagat cgatgttgcc gtttttatca cacgccacaa
    52381 ccaccacctg cattcctgcc atatgtgcag aagcggggtt agttccgtgc gcagaagccg
    52441 ggatcaggca gatatcgcga tgcccttcgt tgcggctttc atgataatga cgaatcgcca
    52501 gcaggcccgc gtattcgccc tgtgcgccgg agttcggctg catacaaacg gcgtcgtaac
    52561 cggtcagttt caccagccag tcagccagct gcgcaatcat ctgctgataa ccttcagcct
    52621 gttccggcgg acaaaacggg tgcagttcgg caaattccgg ccaggtgatt gggatcatct
    52681 cggcggcggc gttcagtttc atggtgcagg aacccagcgg gatcatcgcc tgattcagcg
    52741 ccagatcttt acgctccagc gagtgcatat agcgcatcat ttcggtttcg ctgtggtagc
    52801 gattaaacac cggatgggtg aggatttcgt cgtcgcgcag catcgcaggc tggatagagc
    52861 ggctgtcgtg agccacgtct ttgtccagcg tgtcgatgtc caggccgtgg ttatcgccca
    52921 gtagtacgct gaaaagctgc attacgtttt cacgcgtggt tgtttcatca agggtgatcc
    52981 caaccgcgtt cagaatatcg ctacgcaggt tgatttcagc cgcttcggca cgcgtcagta
    53041 cgcccgcttt gtcggccact tccacacaca aggtgtcgaa atagtgcgca tggcgcagtt
    53101 tcagaccttt ttgttgcagg cccgccgcca ggatatcggt cagacggtga atgcggttag
    53161 cgatacgttt caggccaatc gggccgtgat aaacggcata caggctggcg atgtttgcca
    53221 gcagcacctg ggaagtacaa atgttggagt tcgctttctc acggcgaata tgttgctcgc
    53281 gagtctgcat cgccatgcgc agcgcggtat tgccagctgc atctttcgat acaccgataa
    53341 tacggcccgg cattgagcgt ttgtattcat ctttcgccgc aaagaaggcc gcgtgtgggc
    53401 caccgtagcc catcggcacg ccgaagcgtt gcgccgaacc aaaaacaata tccgcgccct
    53461 gtttacccgg cgcagttaac agcaccagcg ccataatatc ggcggcgaca ctgaccacaa
    53521 ttttgcgtga tttcagttcg ctaataagcg cggtgtagtc gtggatttca ccggttgtgc
    53581 caacctgctg taacagcacg ccgaagacgt cctgatggtc gagcactttt tgcgcgtcat
    53641 cgacaatcac ttcaaaacca aaggtttcgg cacgagtacg gaccacatcc agcgtttgcg
    53701 gatgcacatc ggaagccacg aagaagcggt tggcattttt cagtttgctg acgcgtttcg
    53761 ccatcgccat tgcttcggcg gcagcggtgg cctcgtccag aagagaagca gaggccatat
    53821 ccagtccagt caaatccagc gttacctgct ggaagttgag cagtgcttca aggcggccct
    53881 gggagacttc aggttgatac ggagtgtacg cggtatacca gcccggattt tccagcatgt
    53941 tacgcaggat aaccggcggt agctgcacgg cggtgtaacc catgccgatg taagacgtga
    54001 agcgtttatt gcgactggca atagccttga gttctgccag tgcggcgtat tcggtcgccg
    54061 gtgcgccaac ctgcggcggg gtcgcaagct gaatatcttt cggcacaatc tggccggtca
    54121 gcgcgtttaa cgattgtgca ccaacggcat tcagcatttc ttgctgttgc gcggcgtccg
    54181 gtccgatatg gcgttcaata aaagcgccgc tgttttcaag ctggcttaac gtctgtgtca
    54241 tgagcgatgg ttcctgaaac gtgcagtgaa ttgtgaacct ctctccttac gaagagagtg
    54301 agggtgaggc ataaattttc ctcaccctga tcctctcccg cagaagagga ataaagccgt
    54361 tactcgtctt ctaacaatgc ttcgtatgcg gtcgcatcca gcagtgattc cagttcgctt
    54421 tcatcgctgg ctttgatttt aaagatccag ccacctgcat acggttcgct gttcaccagt
    54481 tccggggaat cgctcagtgc gtcgtttacc gccacgattt caccgcttac tggcgcataa
    54541 atgtctgacg ccgcttttac tgattcggca accgcgcagt catcgcccgc gctaaccgtt
    54601 gcgcccactt ccggcaggtc aacaaacacc atatcgccta acagctcctg ggcatgttcg
    54661 gttataccaa cggtgtaagt gccgtcggct tctttacgca gccattcgtg ttctttgctg
    54721 tatttcagtt ctgctggtac gttgctcatc aatcaatctc caaaaaagta aatcacgcga
    54781 cggctttacc gttacgcaca aaaacaggtt tcgtcacttt aaccggcatt tcacggttgc
    54841 gaatttgcac aatcgccgtt tcgccaatac cttccggcac gcgtgccagc gcaatgctgt
    54901 aacccagcgt cggggagaaa gtcccgctgg tgataatgcc ttcatgctgg ttgccctgcg
    54961 catcggtaaa acgtaccggc agttcattac gcagcacgcc tttttcggtc atcaccagac
    55021 caaccagttt ttctgtgcca tgctcacgct gcacttccag ggcttcacga ccgatgaagt
    55081 cacgatctgc cggttcccag gcgatagtcc agcccatgtt ggcggctaaa ggagaaatag
    55141 tttcgtccat ctcctgacca taaagattca tgcccgcttc cagacgcagc gtgtcacgcg
    55201 cgcccagacc gcatggctta acgcccgctt ccaccagcgc acgccagaaa tcggccgctt
    55261 tttcattggg cagcgcaatt tcatagcccg cttcaccggt ataaccagtg gtggcaataa
    55321 acagatcgcc cgcctgcacg ccaaagaacg gtttcatccc ttccaccgcc tgacgctgag
    55381 catcattaaa cagtgtggca gcttttgcct gcgcattcgg cccttgtacg gcgatcatgg
    55441 aaaggtcatc acgaacggta atttcgatgc cgaaaggttc agcgtgttgg gtaatccagg
    55501 agaggtcttt ttcgcgggtg gcggagttaa caacgaggcg gaagaaatct tcagtaaagt
    55561 agtagacgat gaggtcatct atcacaccgc cagaggcatt caacatcccc gagtaaaggg
    55621 ctttgccgct tttggtgagc ttcgccacat cgttcgccag cagataacgc agaaactccc
    55681 gggtgcggct gccgcgaaga tcgacgatgg tcatatgtga cacatcaaac attccggcat
    55741 cggtacgtac cgcatgatgt tcgtcgattt gcgaaccgta atgcagcggc atcatccagc
    55801 cgtggaaatc caccatgcga gcgccgcaaa gcgtgtgttg ttcgtacaaa ggagtctgtt
    55861 gtgccatctt gtcctcattg aataagcggg gctgacaact ttttcatggt gaaattatca
    55921 ccacgaaacc cagcatcgga gccactcccg gtccccaacg caatcgttct cttttgcctg
    55981 aacttaccac cgaaacagac tgttaaccat aaggtaaaat tgatcatcac attagcttat
    56041 ggttaaaaaa cgccaaaatc gcaacagaat aaaaaaccaa aaaatacaca agtttttata
    56101 taaagatgat gtgatgagaa agttaatttg aataaggcaa tattaagagc tgaaaaaatg
    56161 tcaaaaaaca ctaaatcaaa aattcgtgac attagaaaat ataatgcgaa aacggaggtg
    56221 aaattagttt atttcaaatg aggaaaatct cccggcgaaa aaaccgggag atgaaagtgt
    56281 gatgggtatc aaataaacag cagaagagaa atttttaacg cagccattca ggcaaatcgt
    56341 ttaatcccat tgcctggcgg ataagttgtg gtttaacgcc aggaagcgtg tcggccagtt
    56401 tcaaaccaat atcacgcagc agttttttcg ccggattggt accggaaaac agatcgcgga
    56461 atccctgcat accagccagc atcagcgccg cactgtgctt gcggctacgc tcataccggc
    56521 gcaggtaaat gtactgcccg atgtctttcc cctgacgatg caaccgtttc agttcagcaa
    56581 tcagctctgc agcatccata aagccgagat ttactccctg ccccgccagc gggtgaatgg
    56641 tatgcgcggc gtcgcccacc agcaccagac ggtgcgcggc aaactggcgc gcataacgcc
    56701 ccgtcagtgg gaacacctga cgcgcgctct caaccttgca taagcccagg cgattatcaa
    56761 aagcgatatt taacgcgcga ttaaattcat cttcacttgc ctgctgcatc cgctgcgctt
    56821 cctctggcga cagtgaccag acaattgagc aaagatgcgg atcgctaagc ggtaaaaagg
    56881 ccagaatgcc ttcgccatgg aaaacctgcc gcgccaccgc atcatgcggt tcttccgtgc
    56941 gaatggtcgc taccagcgcg tgatgctgat aatcccagaa agtcagcgga atatctgctt
    57001 tgttgcgcaa ccaggaatta gcaccgtccg cgccaatcac cagacgcgcc gttaacatgc
    57061 tgccatcttt cagcgtcagg aaagtttcat tttctcccca ggcgacctgc tgtaattctg
    57121 cgggggccaa cagagtgata tctgacgact gctgcgcttt gttccacagc gcgtagtgaa
    57181 tcactgagtt ttcaacgata tgcccaagat ggctatagcc catgctttga tcgtcaaacg
    57241 aaatgtgacc aaaactgtct ttgtcccaca cttccatacc gtgataacaa ctggccctac
    57301 gagagagaat gtcctgccag acgccaagac gggtgagtaa tttttcgctg gcggcattga
    57361 tagccgaaac gcgcagttgt ggtggtgcat ccgccgccag aggttccggt acgcgctgct
    57421 ccagtacggc aacgcgtaag ccgctcccct gtaagccgca ggcaaccgcc agccccacca
    57481 tgccgccacc aacaatggct acatcaacac tttgcattgt ttattcctta aaaccgcctt
    57541 caacgcgcca cccaaccgag ggtgcgctgc gccagcacat cgcgtgccgg ggtgaataat
    57601 tccatcgtca tcagtccgat gttgcgcccg acaaccagcg gtgcccaacg gttggcaaaa
    57661 agatgtacca ggctgtccgt gacgccgatg gttgcttcgc gatcactctg tcgacgctgc
    57721 tgatagcggc acaatatgcc gtaatcaccg atgtcttctc cgcgctcatg cgcctgagtc
    57781 agggtttccg caaggctcat gacatctcgc ataccgaggt taaacccttg cccggcaatc
    57841 gggtgcagag tttgcgccgc attgcccacc agcacggtac gatgggtaat agctctggcg
    57901 gcgcgggtta acgccagcgg ataagcactg cgtttaccgg cgtgggtaat ttttccaagt
    57961 cgccagccaa aggccgactg gagttcacgg caaaacttct cgtcgctcca cgacagcacc
    58021 tcttcgcgcc gttccagtgg atgacaccag accagcgaac agcgtccgtc agacatcggc
    58081 aacatcgcca gcgggccatg ttgcgtgaag cgttcaaaag cgcggccttc gtgcgcaacg
    58141 gaagtcgcaa cgttggcaat cacggccagt tgttcgtaag gctcctgctg ccagtcaacg
    58201 ccgcacgcgg tggctaacgc tgaatgggtg ccatcagctg ctaccagcac gcggcccgtc
    58261 agcgtctcgc cactctccag cgtcacttca acgtgactct gagtacgggc aacgttagcc
    58321 acgcgatcag ggcaatgcag cgttacgcca ggtgctttac gcagcaatgc aaacagccgt
    58381 tgcccgacat tgtgcaattc gacaacctgt cccagcgccg ccagttggta atcttctgcg
    58441 gcgagggtga caaatccagc gtgaccacga tcgctgacat gcacggtagt gatggcggtt
    58501 gcgtaatccg ccagagattg ccagacgccg atgcgcgcca gttgctgaca ggtccccgca
    58561 gccagcgcaa tagctcgtcc atcaaagccc ggatgagcat gtgactctgg cgcggtcgct
    58621 tcaatcaaat gtaccggcag cgccccgtga cttaaccggg aaatagccag cgccagcgtc
    58681 gcgcccgcca tgccgccacc gacgatgatt acgctcattg ctttctcgca gctgccatca
    58741 acgcttcgat ttcttccggc tttttcacca cgctggcggt gaggttttcg ttgccggttt
    58801 cggtaatcac aatgtcgtct tcaatacgaa tgccgatacc gcgatattgt tctggcacat
    58861 ccgcatccgg cgcgatatac agaccaggct ctacggtcag taccatcccc ggttccagaa
    58921 tgcgcgagcg atcctgacca taaacgccaa cgtcatggac atctagccct aaccagtggc
    58981 taaggccatg cataaagaaa ggacgatggg cgttctgagc gatcagttca tcaacatcac
    59041 ctttcagaat gccgagtttt accaggccgc taaccatgat gcgcaccact tcaccagtga
    59101 cttccagaat ggaagttccc ggacgataca ggcgcaagct ggtttcgaga gactccagca
    59161 caatgtcgta gatttcacgc tgggcctgag tgaatttgcc gttgaccggg aaggtgcggg
    59221 taatatcgcc agcgtaacct ttgtattcgc aacccgcgtc aatcaacacc aggtcgccgt
    59281 cgcgcatttc acactcgttt tcggtgtagt gcagaatgca gccgttttca ccgctgccga
    59341 caatggtgtt ataggacgga tagcgcgcac cgtggcggtt aaattcgtgg tgaatttcgc
    59401 cttccagatg gtactcgaac attcccggac ggcatttttc catcgcccgg gtatgtgcca
    59461 tggcggtgat ttctcccgcg cggcggagta cggcaatctc ttctggcgat ttgaacaggc
    59521 gcatttcatg aacaacagga cgccagtcga tcatcgttgc cggtgcggtg agattttgcc
    59581 gcgaaccttt acgcagtttt tccagcgcac tgttcacgat ttcatcagca tatgcatatt
    59641 cgccctgggc atggtaaacc acatccaggc cgttaagtag ttgataaagt tgctgattga
    59701 tttcgctgaa tgccagtgcg cggtcaacgc ccagtttctc tggcgcggca tcctggccta
    59761 agcgacggcc aaaccagatc tccgccgtca ggtcgcgaac gcggttaaac agaacgctgt
    59821 ggttatgagt gtcatcgctt ttaatcagca ccagcaccgc ttccggttcg ttaaagccgg
    59881 tgaagtacca gaagtcactg ttctgacgat aggggtattc gctgtcggcg ctacgtgtta
    59941 cttctggtgc agcaaaaatc agcgcggcgc tgccgggttg catttgctcc accagggcct
    60001 gacggcgacg ctgaaactct tgccgggata tctcactcat aacactctcc ttacgttttt
    60061 tgtttttagt gtagagtcgg tttttgtact tctggcgcgg tcggttgcgg atgagtaaag
    60121 gtgtcgtggc ataacagcgc ggcaacacga acgtattcga tgatctcttc aagcgacatt
    60181 tcaagctctt cctgatcttc gtcttcgtcg taacccagtt gcgcaatgtt acgcagatcg
    60241 tcgatagctt caccggtttc gccggtcact ttatccagct tcggttgcgt aacgccaaga
    60301 ccaagcagga agtgattgac ccaacctgcc aatgcatcag cccgatcgaa aacgctgaca
    60361 tcatcgccat caggcagata aagctgaaaa aggaagccgt catcctgcag ggcatcgctg
    60421 gtggcagagt gcattttacg cagtgcctgt gccagctcat gaccgaaagc catgccttcg
    60481 ttcgtcaggt cgtgaagtag cggtagccat gagctgtcat cgttaccgcc acatatcatc
    60541 ccactgatta aaccatgcat ctcagcaggg gtcagacccg tcccttgttg gttcagatac
    60601 tggttcattt cgttgtaacc aggcatttcg ttctgtatag acataagcat tcgtcgtcaa
    60661 agggaggaat attcatgata tgctaccact ttgggccctg gtggaccaga aaagggcttg
    60721 tctcttctca tcagggtagc tatagtgtcg ccccttcgca gaccatgggt ctaaagacga
    60781 aggcagcgca gtcaatcagc aggaaggtgg catgtctgca caacccgtcg atatccaaat
    60841 ttttggccgt tcactgcgtg tgaactgccc gcctgaccaa agggatgcgt tgaatcaggc
    60901 agcggacgat ctgaaccaac ggttgcaaga tctgaaagaa cgcactagag tcacaaatac
    60961 tgaacagttg gtcttcattg ccgcattgaa tatcagctat gagttagcgc aagaaaaagc
    61021 gaagactcgt gactacgcgg caagtatgga acagcgtatt cggatgctgc agcagaccat
    61081 agaacaagcg ttacttgaac aaggtcgcat caccgaaaaa actaaccaaa actttgaatg
    61141 acacttttcg gtttactgtg gtagagtaac cgtgaagaca aaatttctct gagatgttcg
    61201 caagcgggcc agtcccctga gccgatattt cataccacaa gaatgtggcg ctccgcggtt
    61261 ggtgagcatg ctcggtccgt ccgagaagcc ttaaaactgc gacgacacat tcaccttgaa
    61321 ccaagggttc aagggttaca gcctgcggcg gcatctcgga gattcccttc ttatctggca
    61381 ccagccatga cgcaactacc agaactccca ctgacattat cccgacaaga aatccgcaaa
    61441 atgattcggc aacgtcgtcg tgcgttaacg ccggaacaac agcaggaaat gggtcaacaa
    61501 gccgctaccc ggatgatgac ttatcccccg gtggtgatgg cgcatacggt cgctgtattc
    61561 ctctcttttg atggtgaact cgacacccag ccgctaatag aacaactctg gcgcgccggt
    61621 aagcgcgtat atcttccagt tttgcatccc tttagtgccg gtaatttgct gttcctgaat
    61681 taccatccgc aaagcgaact ggtgatgaac aggttgaaga tccatgagcc aaaactggat
    61741 gtgcgtgacg tgctacccct ttcccgatta gacgtgctga tcacaccgct ggtcgccttt
    61801 gatgagtacg gtcagcgcct gggaatgggc ggtggttttt atgatcggac cttacaaaac
    61861 tggcagcact ataaaacgca accggtgggt tatgcgcatg attgtcagtt ggtggaaaaa
    61921 ctccccgttg aagagtggga tatccctctt cctgcggtgg ttacaccgtc gaaagtctgg
    61981 gagtggtaag ggcgatacac ctgcatcgcc ctgattgaca tcgttgattc tttaacctaa
    62041 tttagtgagt aagggtaagg gaggattgct cctcccctgt gactgactgt taataagctg
    62101 ggaaacttat gagtaacagt acaatcagta tgatggcaag tcgcatcata acccttctcc
    62161 ttcaagccct cgcttcggtg agggctttac cgttacagcc ccatgctgcc cagctatcgt
    62221 aagctctctt taaataacca caacgcattg atctaccttt aaatcttttc ttcgagcgtg
    62281 ctcgaaaaac cccatgcaaa acagagataa tttttgggaa aaaggatcta ttacggcagt
    62341 aaacgtgggc gatgaagtgg ctatcaaaaa gttcggttca gggcgatgca ccccgcatcg
    62401 ccctgattga catcgttgat tctttgacct aatttagtga gtaagggtaa gggaggatga
    62461 ctcctcccct gagactgact gttaataagc tccgaaactt atcagtaaca gtacaatcag
    62521 tatgatgaca agtcgcatca taacccttct cctttaagcc ctcgcttcgg tgagggcttt
    62581 accgttacag ccccatgctg tcccgccatc gtaaatcccc attaaataaa cacaacgcat
    62641 tgatctgact ttgatttatt ttctggagca gactcgcaaa gtagaatgcg caacgcggca
    62701 acggtgtgga gaagggataa aaaaacgggc aagtcagtga cctgcccgtt aattttcaga
    62761 gaaggggaat tagtacagca gacgggcgcg aatggtaccc ggaatagctt tcattgcctg
    62821 cagcgctttt tcggcaacgt cttcgtcagc ttcaatatca ataactacat aacccatctg
    62881 ggcggaagtt tgtagatatt gcgcggcgat gttgacgccc tgctcggcga agattttgtt
    62941 cagcgcagtt agcacgcccg gacggttttc gtggatgtgc atcagacgac gaccaccatg
    63001 cagtggcagc gagacttccg ggaagttcac cgcagagagc gttgagccat tgtcagaata
    63061 cttgatcaat ttacccgcaa cttccaggcc gatattctcc tgcgcttcct gagtcgaacc
    63121 gccaatgtgt ggcgtcagaa ggacgttgtc gaattcacac agtggagagg taaatggatc
    63181 gctattggtc gccggttccg tcgggaatac gtcgattgcc gcccccgcca gatgtttgct
    63241 cgccagcgca tcacacagcg ccggaatatc caccacagta ccgcgcgagg cattaatcag
    63301 cagcgagccg ggcttcatta gcgaaatctc tttcgcgccc atcatatttt tggtggacgg
    63361 attctctggt acatgcagac tcaccacatc gctcatattc agcaggtcag aaagatgctg
    63421 tacctgagtg gcgttgccca gcggcagttt gttttcaata tcataaaagt aaacatacat
    63481 tcccagcgat tcagccagaa tgcccaattg cgtaccaata tgaccgtagc cgatgatacc
    63541 cagctttttg ccgcgcgctt caaaagaacc cgccgccagt ttgttccaca cgccacggtg
    63601 cgctttagca ttggcttccg gcacgccgcg caatagcagc agcagttcgc caatcaccag
    63661 ctccgcaaca gagcgcgtat ttgagaacgg tgcgttaaat accgggatcc cgcgctttgc
    63721 cgccgcatcc agatcaacct ggtttgttcc gatacagaaa cagccaatag cgaccagttt
    63781 ttctgcggcg ttgatcacgt cttcagtcag atgggtacgg gatcgcaggc cgatgaagtg
    63841 ggcatcgcgg atggattctt ttaattgttc atcatccagc gcgcctttgt gaaattcgat
    63901 gttggtgtaa ccagctgcac gaaggctttc cagcgccttt tggtgcacgc cttctaccag
    63961 cagaaactta atcttgtctt tctccagcga tacctttgcc atttacccaa tcctgtcttt
    64021 tgaaatgttg tgtgcggatt tgcatccgcc tttcaacata tcaaaaaata atattgcggc
    64081 aatatgaacg tttgcgtcgc gatgttgagg aaatatcacg caaaggtaat tcttggatga
    64141 atatgctggt ttagaggatt tatttagagc aatcgacaat tgcctggtaa aagcgtgaca
    64201 catgtcacca aatttaatga agagaatttt tttaacgggg gaggttcccc cgtcagatca
    64261 tttcacaatg gttttgacac cgtcaggtgt gccaatcagc gcaacgtccg cgccacggtt
    64321 agcaaacaag ccaacagtca ccacgccagg aatcgcattt atggcgtttt ccatcgctat
    64381 cgggtcaagg atttccatgc cgtggacgtc gaggatcacg ttgccattat cggtcaccac
    64441 gccctgacgg tattccggac gaccgcccag tttcaccagc tgacgcgcca ctgcactacg
    64501 tgccatcggg ataacttcta ctggcagcgg gaatttaccc agaatatcaa cctgcttgga
    64561 agcgtctgca atacagataa atttttctgc aaccgaagca atgatttttt cacgggtcag
    64621 cgccgcgccg ccgcctttga tcatttgcat gtggccgttg atttcatctg cgccatcaac
    64681 gtagatgcca aggctgtcga cttcgttgag atcaaaaacg tgaatgccga ggcttttcag
    64741 tttttcagtg gaagcatctg aactggaaac ggccccttca atctggcctt tcattgtacc
    64801 gagcgcgtca ataaagtgtg cggcggtgga acctgtacct acaccaacaa tggtgccggg
    64861 ctgaacatac tgaagtgccg cccatcctac tgcttttttc aattcatcct gcgtcatgat
    64921 cgtttcgcct gtggtatgaa atttcacacg cattatatac aaaaaaagcg attcagaccc
    64981 cgttggcaag ccgcgtggtt aactcatcca taaaatatcg cgcaatggcg ggcatcccct
    65041 ttcgccccgc aaataaagca tacaacggtc tgggtatgcc gctccacggt gcgaacagag
    65101 gcaataacgc cacgccattc aacacaacca gggcgcgaac tcgctgagcg aaatgcatta
    65161 gcgcaaatcc ctgatctttt aagctgcgcg tttaaccacg aatacaaatg ttacccgccg
    65221 acggcttcgg tatatgcaac ctgacacaaa attgtgtcat agtgcaggaa aaagcattta
    65281 ccaggagcag acaacagcaa tgaaacgccc ggactacaga acattacagg cactggatgc
    65341 ggtgatacgt gaacgaggat ttgagcgcgc ggcacaaaag ctgtgcatta cacaatcagc
    65401 cgtctcacag cgcattaagc aactggaaaa tatgttcggg cagccgctgt tggtgcgtac
    65461 cgtaccgccg cgcccgacgg aacaagggca aaaactgctg gcactgctgc gccaggtgga
    65521 gttgctggaa gaagagtggc tgggcgatga acaaaccggt tcgactccgc tgctgctttc
    65581 actggcggtc aacgccgaca gtctggcgac gtggttgctt cctgcactgg ctcctgtgtt
    65641 ggctgattcg cctatccgcc tcaacttgca ggtagaagat gaaacccgca ctcaggaacg
    65701 tctgcgccgc ggcgaagtgg tcggcgcggt gagtattcaa catcaggcgc tgccgagttg
    65761 tcttgtcgat aaacttggtg cgctcgacta tctgttcgtc agctcaaaac cctttgccga
    65821 aaaatatttc cctaacggcg taacgcgttc ggcattactg aaagcgccag tggtcgcgtt
    65881 tgaccatctt gacgatatgc accaggcctt tttgcagcaa aacttcgatc tgcctccagg
    65941 cagcgtgccc tgccatatcg ttaattcttc agaagcgttc gtacaacttg ctcgccaggg
    66001 caccacctgc tgtatgatcc cgcacctgca aatcgagaaa gagctggcca gcggtgaact
    66061 gattgactta acgcctgggc tatttcaacg acggatgctc tactggcacc gctttgctcc
    66121 tgaaagccgc atgatgcgta aagtcactga tgcgttactc gattatggtc acaaagtcct
    66181 tcgtcaggat taatccatca aataatgcct gatagcacat atcaggcgtt gtcctcactt
    66241 ctttttgtat tccttgaatc acatcacaaa atagacaaat ctcaggcggc aaaaaacgac
    66301 gtctgaatgc attttttttg ctggcgacaa acccacgtaa aaagctcacc gtaggcgcaa
    66361 ataccctcat tttgattgcg ttttacggag caaataatgt ctaacgtgca ggagtggcaa
    66421 cagcttgcca acaaggaatt gagccgtcgg gagaaaactg tcgactcgct ggttcagcaa
    66481 accgcggaag ggatcgccat caagccgctg tataccgaag ccgatctcga taatctggag
    66541 gtgacaggta cccttcctgg tttgccgccc tacgttcgtg gcccgcgtgc cactatgtat
    66601 accgcccaac cgtggaccat ccgtcagtat gctggttttt caacagcaaa agagtccaac
    66661 gctttttatc gccgtaacct ggccgccggg caaaaaggtc tttccgttgc gtttgacctt
    66721 gccacccacc gtggctacga ctccgataac ccgcgcgtgg cgggcgacgt cggcaaagcg
    66781 ggcgtcgcta tcgacaccgt ggaagatatg aaagtcctgt tcgaccagat cccgctggat
    66841 aaaatgtcgg tttcgatgac catgaatggc gcagtgctac cagtactggc gttttatatc
    66901 gtcgccgcag aagagcaagg tgttacacct gataaactga ccggcaccat tcagaacgat
    66961 atcctcaaag agtacctctg ccgcaacacc tatatttacc caccaaaacc gtcaatgcgc
    67021 attatcgccg acatcatcgc ctggtgttcc ggcaacatgc cgcgatttaa taccatcagt
    67081 atcagcggtt accacatggg ggaagcgggt gccaactgcg tgcagcaggt agcatttacg
    67141 ctcgctgatg ggattgagta catcaaagca gcaatctctg ccggactgaa aattgatgac
    67201 ttcgctcctc gcctgtcgtt cttcttcggt atcggcatgg atctgtttat gaacgtcgcc
    67261 atgttgcgtg cggcacgtta tttatggagc gaagcggtca gtggatttgg cgcacaggat
    67321 ccgaaatcac tggcgctgcg tacccactgc cagacctcag gctggagcct gactgaacag
    67381 gatccgtata acaacgttat ccgcaccacc attgaagcac tggctgcgac gctgggcggt
    67441 actcagtcac tgcataccaa cgcctttgac gaagcgcttg gtttgcctac cgatttctca
    67501 gcacgcattg cccgcaacac ccagatcatc atccaggaag aatcagaact ctgccgcacc
    67561 gtcgatccac tggccggatc ctattacatt gagtcgctga ccgatcaaat cgtcaaacaa
    67621 gccagagcta ttatccaaca gatcgacgaa gccggtggca tggcgaaagc gatcgaagca
    67681 ggtctgccaa aacgaatgat cgaagaggcc tcagcgcgcg aacagtcgct gatcgaccag
    67741 ggcaagcgtg tcatcgttgg tgtcaacaag tacaaactgg atcacgaaga cgaaaccgat
    67801 gtacttgaga tcgacaacgt gatggtgcgt aacgagcaaa ttgcttcgct ggaacgcatt
    67861 cgcgccaccc gtgatgatgc cgccgtaacc gccgcgttga acgccctgac tcacgccgca
    67921 cagcataacg aaaacctgct ggctgccgct gttaatgccg ctcgcgttcg cgccaccctg
    67981 ggtgaaattt ccgatgcgct ggaagtcgct ttcgaccgtt atctggtgcc aagccagtgt
    68041 gttaccggcg tgattgcgca aagctatcat cagtctgaga aatcggcctc cgagttcgat
    68101 gccattgttg cgcaaacgga gcagttcctt gccgacaatg gtcgtcgccc gcgcattctg
    68161 atcgctaaga tgggccagga tggacacgat cgcggcgcga aagtgatcgc cagcgcctat
    68221 tccgatctcg gtttcgacgt agatttaagc ccgatgttct ctacacctga agagatcgcc
    68281 cgcctggccg tagaaaacga cgttcacgta gtgggcgcat cctcactggc tgccggtcat
    68341 aaaacgctga tcccggaact ggtcgaagcg ctgaaaaaat ggggacgcga agatatctgc
    68401 gtggtcgcgg gtggcgtcat tccgccgcag gattacgcct tcctgcaaga gcgcggcgtg
    68461 gcggcgattt atggtccagg tacacctatg ctcgacagtg tgcgcgacgt actgaatctg
    68521 ataagccagc atcatgatta atgaagccac gctggcagaa agtattcgcc gcttacgtca
    68581 gggtgagcgt gccacactcg cccaggccat gacgctggtg gaaagccgtc acccgcgtca
    68641 tcaggcacta agtacgcagc tgcttgatgc cattatgccg tactgcggta acaccctgcg
    68701 actgggcgtt accggcaccc ccggcgcggg gaaaagtacc tttcttgagg cctttggcat
    68761 gttgttgatt cgagagggat taaaggtcgc ggttattgcg gtcgatccca gcagcccggt
    68821 cactggcggt agcattctcg gggataaaac ccgcatgaat gacctggcgc gtgccgaagc
    68881 ggcgtttatt cgcccggtac catcctccgg tcatctgggc ggtgccagtc agcgagcgcg
    68941 ggaattaatg ctgttatgcg aagcagcggg ttatgacgta gtgattgtcg aaacggttgg
    69001 cgtcgggcag tcggaaacag aagtcgcccg catggtggac tgttttatct cgttgcaaat
    69061 tgccggtggc ggcgatgatc tgcagggcat taaaaaaggg ctgatggaag tggctgatct
    69121 gatcgttatc aacaaagacg atggcgataa ccataccaat gtcgccattg cccggcatat
    69181 gtacgagagt gccctgcata ttctgcgacg taaatacgac gaatggcagc cacgggttct
    69241 gacttgtagc gcactggaaa aacgtggaat cgatgagatc tggcacgcca tcatcgactt
    69301 caaaaccgcg ctaactgcca gtggtcgttt acaacaagtg cggcaacaac aatcggtgga
    69361 atggctgcgt aagcagaccg aagaagaagt actgaatcac ctgttcgcga atgaagattt
    69421 cgatcgctat taccgccaga cgcttttagc ggtcaaaaac aatacgctct caccgcgcac
    69481 cggcctgcgg cagctcagtg aatttatcca gacgcaatat tttgattaaa ggaattttta
    69541 tgtcttatca gtatgttaac gttgtcacta tcaacaaagt ggcggtcatt gagtttaact
    69601 atggccgcaa acttaatgcc ttaagtaaag tctttattga tgatcttatg caggcattaa
    69661 gcgatctcaa ccggccggaa attcgctgta tcattttgcg cgcgccgagt ggatccaaag
    69721 tcttctccgc aggccacgat attcacgaac tgccgtctgg cggtcgcgat ccgctctcct
    69781 atgatgatcc attgcgtcaa atcacccgca tgatccaaaa attcccgaaa ccgatcattt
    69841 cgatggtgga aggtagtgtt tggggtggcg catttgaaat gatcatgagt tccgatctga
    69901 ttatcgccgc cagtacctca accttctcaa tgacgcctgt aaacctcggc gtcccgtata
    69961 acctggtcgg cattcacaac ctgacccgcg acgcgggctt ccacattgtc aaagaactga
    70021 tttttaccgc ttcgccaatc accgcccagc gcgcgttggc tgtcggcatc ctcaaccatg
    70081 ttgtggaagt ggaagaactg gaagatttca ccttacaaat ggcgcaccac atctctgaga
    70141 aagcgcagtt agccattgct gttatcaaag aagagctgcg tgtactgggc gaagcgcaca
    70201 ccatgaactc cgatgaattt gaacgtattc aggggatgcg acgcgcggtt tatgacagcg
    70261 aagattacca ggaaggaatg aacgctttcc tcgaaaaacg taaacctaat ttcgttggtc
    70321 attaatccct gcgaacgaag gagtaaaaat ggaaactcag tggacaagga tgaccgccga
    70381 tgaagcggca gaaattatcc agcataacga catggtggca tttagcggct ttaccccggc
    70441 gggttcgccg aaagccctac ccaccgcgat tgcccgcaga gctaacgaac agcatgaggc
    70501 caaaaagccg tatcaaattc gcctgctgac gggggcgtca atcagcgccg ccgctgacga
    70561 tgtactttct gacgccgatg ctgtttcctg gcgtgcgcca tatcaaacat cgtccggttt
    70621 acgtaaaaag atcaatcagg gcgcggtgag tttcgttgac ctgcatttga gcgaagtggc
    70681 gcaaatggtc aattacggtt tcttcggcga cattgatgtt gccgtcattg aagcatcggc
    70741 actggcaccg gatggtcgag tctggttaac cagcgggatc ggtaatgcgc cgacctggct
    70801 gctgcgagcg aagaaagtga tcattgaact taatcactat cacgatccgc gcgttgcaga
    70861 actggcagat attgtgattc ctggcgcgcc accgcggcgc aatagcgtgt cgatcttcca
    70921 tgcaatggat cgcgtcggta cccgctatgt gcaaatcgat ccgaaaaaga ttgtcgccgt
    70981 cgtggaaacc aacttgcccg acgccggtaa tatgctggat aagcaaaatc ccatgtgcca
    71041 gcagattgcc gataacgtgg tcacgttctt attgcaggaa atggcgcatg ggcgtattcc
    71101 gccggaattt ctgccgctgc aaagtggcgt gggcaatatc aataatgcgg taatggcgcg
    71161 tctgggggaa aacccggaaa ttcctccgtt catgatgtat tcggaagtgc tacaggaatc
    71221 ggtggtgcat ttactggaaa ccggcaaaat cagcggggcc agcgcctcca gcctgacaat
    71281 ctcggccgat tccctgcgca agatttacga caatatggat tactttgcca gccgcattgt
    71341 gttgcgtccg caggagattt ccaataaccc ggaaatcatc cgtcgtctgg gcgtcatcgc
    71401 tctgaacgtc ggcctggagt ttgatattta cgggcatgcc aactcaacgc acgtagccgg
    71461 ggtcgatctg atgaacggca tcggcggcag cggtgatttt gaacgcaacg cgtatctgtc
    71521 gatctttatg gctccgtcga ttgctaaaga aggcaagatc tcaaccgtcg tgccaatgtg
    71581 cagccatgtt gatcacagcg aacacagcgt caaagtgatc atcaccgaac aagggatcgc
    71641 cgatctgcgc ggtctttccc cgcttcaacg cgcccgcact atcattgata attgtgcaca
    71701 tcctatgtat cgggattatc tgcatcgcta tctggaaaat gcgcccggcg gacatattca
    71761 ccacgatctt agccacgtct tcgacttaca ccgtaattta attgcaaccg gctcgatgct
    71821 gggttaatgg tttgtcccgt cacgatcttc agcaatatgc tgaagcatcg tggcggtata
    71881 acgatgattt ctgattgaat aaagatattc ctgcatatac atcggcctgt gcggcgcatc
    71941 aaaatatttc agcgttgccg gattcaccag tcgccaggca aaatgcggga tcaccgtcag
    72001 aaaatgtccg cgctccacgg cgctgatttt ggccataaaa ctgtagggac gataaataat
    72061 ggtgggattg atcccacaag gtcgcatatt cgcatcaatc attgcttcga agttagcccg
    72121 gttttgaaag cgcatttgta gccagggcag ctcctgaagc agctcttgct cttgacgctc
    72181 ttcataacgg cgagaaacga gaaagcccaa tcgcagcggt ggtaattcac tgatagtcag
    72241 gttttccagt tcctgaactc gtgcagatac atgctgtggg gagataataa aatctaactg
    72301 acggtcaaaa agattatcga taacaccgtt ttcgctgaac tcaactggct gggcggtcac
    72361 gccttcatac ttatctccga gactaatcaa ctgatcgaaa ataattgtcg gataagtatt
    72421 atctatgcca atcacaattt ctcgtgaacg acgccctgag ttatggattt cattatcaat
    72481 cgcagaaagt cgttgataaa ccgggaacag tttttgatac agttcctgac cggctttatt
    72541 gaggctgata ttattatctt tgcgggtaaa tagcgtatag ccaatttgtt cttccagcgc
    72601 ggcaatgctt ttaccaaatg gcgaagccgt catatggatt ttttctgccg cccgggcaat
    72661 attattggtt tgcgctaata aaatgaaatt gcgcatcttt ttcgaaataa aaatgtccat
    72721 cccccctccc cgcatccttg tccgcttaac cgtttcagtc agcctatcct tgatgaaacc
    72781 gcgagcaaag ataggtgatt acgtcatggt tttacagaaa attacagaaa aaggaggcaa
    72841 tatcgggtaa aggcattagc ccgacgaata cgtcgggcta caagtattat tgtgctgcag
    72901 gtgttttagc gggttgttga tccacaggtt ctaactggaa gaccacatcg acctgatcat
    72961 caaactgaat agcggcctgc tcgtaagttt cctgggcaga caccggcgcg gcatcggctt
    73021 tcatcatccg caccattggg ctgggctgat agttggaaac atggtagcgc acgctatata
    73081 ccggccccag tttacgatga aagccgttcg ccagttcctg cgcctgatga atcgcgttat
    73141 caatcgctgc cttacgcgct ttgtctttat aggcatccgg ctgcgccacg cccagcgaca
    73201 cagaacgaat ttcgttaaga cccgccttca gcgcgccatc cagcaaggaa ttcagtttgt
    73261 ctaactgacg gagcgtgact tccaccgttc tcacagcgcg gtagcctttc aggatacttt
    73321 taccatcctg ataatcataa tctggctggg tgcgtaaatt cgctgagctg atatcttttt
    73381 tcgcgatctg attgagttca aggaaggaaa tgtattgtgc gacgcgctca tctgcctgtt
    73441 tcttggctgt agcggcatcc ttcgcggcca cgttaacttc aatcgcaaga gtggcaatgt
    73501 ctggcaccgc atccacactt gccgtaccgg aggtgacaat atgcggtcca tccggcaatt
    73561 cgttagcctg cgctgccatc ccgctaatac ccattaatgc cgccagggcg ataactttga
    73621 acttcacgtt agtctctccg tgttaattga cttgcccaaa attcaggacc tctggaggca
    73681 agcttagcgc ctctgtttta tttttccatc agatagcgcc taactgaaca aggcttgtgc
    73741 atgagcaata ccgtctctcg ccagctgcaa ggctataaac cacatgacac atcccactac
    73801 cagattgata atgcgctgtg attttgccgt gcgcaggcgc ggtgccagcc aggctgcgag
    73861 aatagccaga ccaaagaacc acaggaaaga ggcgctaatt gtcccgagcg caaaccaacg
    73921 ttttggttcg acgtcaagct gcccgccaag gctgcccagt acaacaaaag tatccaggta
    73981 aacatgcgga ttcagccagg tcactgccaa catggtggcg ataattttcc atctgccttg
    74041 cttcaggact tcggcactag ctaactcaat attactactc atcgctgttt taaaagcgcc
    74101 aaaaccatac cacagcaaga agactacgcc gccccaggtg accagcgcca gcaaccacgg
    74161 cgactgcatc aataacgcgc taccaccaaa aatccccgcg caaatcagga ccaaatcgct
    74221 gatagcgcaa agtaaggcaa tcataatgtg gtactgacga cgaatgccct gattcatcac
    74281 aaaagcattt tgtgggccga gcggcagaat catagctgcc ccaagagcaa gaccttgaaa
    74341 gtaataagaa aacacgttgg atattccgaa ttaagtatct gatgcagaga ctatatcgcg
    74401 ggattgttat tagcggaaat tgataatatt aattggcaat cagaataact aataagagag
    74461 aggccagaga gtattatctg gcctcagcgt tgattacgca gctttgtctt ctttcacccg
    74521 cttaaagttc acatccattt gcgggtacgg gaagctgata ccggcggcat caaattcacg
    74581 tttaatacgc tccagcacat cccagtacac gttttgcaga tcgccgctgt tgctccagac
    74641 gcggaccacg aaattaatcg acgatgcacc aagttcgttc aggcgcacag tcatttcgcg
    74701 atctttcaaa atgcgatctt cagactggat aatattggtc aggatctgct taacctgatc
    74761 gatatcggaa tcatacgcca cgccaataat aaattcgtta cggcgaactg gctcgcggga
    74821 gaagttaata atatttccgg caataatttt accgttcgga ataacgataa ttttaccgtc
    74881 tgcagtacgc atggtggtgg agaaaatctg cacactcagc acagtaccgg ctacgccgcc
    74941 gaggtcaaca tattctccgg cacggaacgg gcggaacatg acaagtaaca cgccagcggc
    75001 caggttagaa agtgacccct gcaaagccag accaacagct aagcctgcgg caccgagtac
    75061 agcaatgact gacgctgttt gtacacccac gcgtcccagg gctgcgatta gcgtaaaggc
    75121 gataataccg taacggacta atgcagaaag aaaatcagca acagtggcat cgattttacg
    75181 ggagatcatc aggcgattca ccgcgttgga aatcatccgc gcgataatca aaccaacgat
    75241 gatgatcgcg agtgccgcca cgatgtttac tgcataactt agcagcagcg cctggttagc
    75301 taccagccag cttcccgcgc cgtttatgct atcgacaaca ttcaaatctt ccattcaata
    75361 ttccttttca actgactcaa aatggagaaa tacactccgc cgccttatga cgggcagtct
    75421 gacaagggta aacaataaag cataattttg ccaaatagat cacagatttc tcatctgaat
    75481 caccttgctt ttggcgaaaa aatccccgca gagcggggct cagacagata tcaatgtgcg
    75541 ctttgtcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaaacc tgcaaattca
    75601 atatattgca ggatacgtgt aggcctgata agcgtagcgc atcaggcaat tttgcttttg
    75661 gcgaaaaaaa gacccgcaga gcgggccttg agataagcag aaaggaatga cttacagaac
    75721 gtcgatcgcg ttcagttcct ggaatgcttt ttccagacga gcgatcatcg aagtctgacc
    75781 ggcacgcagc catacgcgcg gatcgtagta tttcttgttc ggctgatctt cgcctttcgg
    75841 gttacccagc tgaccctgca gataagcttc gttcgctttg tagtagttca gaacgccttc
    75901 ccaggttgcc cattgggtat cggtatcgat gttcattttc actacgccgt agcttacgga
    75961 gtctttgatt tcctgagcag tagaaccgga accaccgtgg aatacgaagt tcaggctgtt
    76021 gtgcggcagg ttgtgtttct tagaaacata ttcctgagaa tcacgcagga tggtcggagt
    76081 cagaaccacg ttacccggct tgtaaacacc gtgtacgtta ccgaaggacg cagcgatggt
    76141 gaaacgcggg ctgattttgc tcagctcggt gtatgcgtaa tcaacgtctt ccggctgggt
    76201 gtacagtgca gaagcgtcca tgtggctgtt gtccacgccg tcttcttcac caccggtgca
    76261 acccagttcg atttccagag tcatgccgat tttggacata cgctccaggt atttagagca
    76321 gatttcgatg ttctcttgca gagattcttc agacaggtcg atcatgtgag aagagaacag
    76381 cggcttaccg gtagctgcga agtgtttttc acccgcgtcc agcagaccgt cgatccacgg
    76441 cagcagtttc ttcgcgcagt ggtcagtgtg caggataacc ggaacaccat aatgttcagc
    76501 catctggtga acgtgatgcg caccagagat cgcgcccagg atagcagcac cctgcggaac
    76561 gtcagatttc acgcctttac cagcgataaa ggaagcacca ccgttggaga actgaacgat
    76621 aaccggcgct ttaactttag cagcagtttc cagtacggcg ttgatggagt cagtaccgac
    76681 gcagtttact gctggcagtg cgaagttgtt ttcttttgct acctggaaaa ctttctgtac
    76741 gtcatcacca gtgattacgc caggttttac gaaatcaaaa atcttagaca tgtctcttgt
    76801 cctgtatcgt cgggccttat actcatcata cttcaagttg catgtgctgc gtctgcgttc
    76861 gctcacccca gtcacttact tatgtaagct cctggggatt cactctcttg tcgccttcct
    76921 gcaactcgaa ttatttagag tatgaaaaaa tgcgtgctct aaaagcgcgc tgaaacaagg
    76981 gcaggtttcc ctgccctgtg attttttact tcttagcgcg ctcttcgagc atcgctactg
    77041 caggcagtac tttaccttcc acgaattcga ggaatgcgcc gccgccagtg gagatgtagg
    77101 agattttgtc agcaatgccg aacaggtcga ttgctgccag agtgtcgccg ccgccagcga
    77161 tggagaacgc ttcgctgtct gcgatagcgt tagccacgat ttcagtacct ttgcggaagt
    77221 tcgggaattc gaacacgcca accggaccgt tccacagaat ggttttcgca ttcttcagga
    77281 tttcagccag ttcctgagcg gaagcatcac cgatatccag gatctgctcg tcagctttca
    77341 catcgttaac agatttcagg gtagccggtg cagtttcaga gaactcggtt gctacgcgaa
    77401 catcagacgg aaccgggatg ttgcaggtgg tcagcagacg tttagcttcg tcaaccaggt
    77461 cagcttcgta cagggattta cccacatcgt ggccttgtgc cgcgataaag gtgttagcga
    77521 taccaccacc aacaatcagc tggtcagcga ttttagacaa ggagtccaga acggtcagtt
    77581 tggtagatac tttagaacca ccaacgatag ccaccatcgg gcgagctggt tctttcagtg
    77641 ctttacccag cgcgtccagt tcagctgcca gcagcgggcc tgcgcacgca acgtcagcga
    77701 atttaccgat accgtgagta gaagcctgcg cgcggtgagc agtaccgaat gcgtccatta
    77761 cgaacacgtc acacagtgca gcgtattttt tggacagggt ttcgtcgtct ttcttctcgc
    77821 ctttgttgaa gcgaacgttt tccagaacaa ccagttcacc ttcagcaacg tcaacgccgt
    77881 cgaggtaatc tttaaccagg cgaaccgggt tagacagttt gtctttcagg tagttaacaa
    77941 ccggcagcag agagaattct tcgttgtact cgccttcggt aggacgaccc aggtgggaag
    78001 ttaccatcac ttttgcgcct tgtttcaggg ccagttcgat ggtcggcaga gaagcacgga
    78061 tacgcgcgtc gctggttact ttcccgtctt ttactggtac gttcagatcc gcacggataa
    78121 atacacgttt cccagcaaga tccagatcgg tcatcttaat tacagacatg gtgaatcctc
    78181 tcgttgattc taaaagtttt gcagacgctg cttgcgtctt acctgaaagc aacagtagcc
    78241 atagctaacg tcgtgtcgag cattcggtta gcaaagcccc attcgttatc gcaccagacc
    78301 aacgttttga tcaggtgtgc gccactgacc cgggtttggg tgccatcgac aatggcactg
    78361 tgcggatcgt ggttaaaatc tacagagacc aacggcaatt ccgtatagtc aactatacca
    78421 tgaaatgcac cttgtgctgc tttttgcagc aacaggttga cttcattggc ttttacaggt
    78481 ttcttcaccg tcacgcttaa atcgattgcc gtcacattta tggttggcac acgtaccgca
    78541 atcgcttcaa agcgatcgtt aaattgcgga aaaaatcgtg tgataccggc ggccagttta
    78601 gtatcgaccg gaatgatcga ctggctggct gcccgggtgc gacgcaggtc aggatggtat
    78661 gcatcaataa cctgttgatc gtgcatggcg gagtgaattg tggtcacagt gccggactca
    78721 ataccgtacg catcatctaa caatttgatg acgggaatta tgcaattcgt ggtacaggaa
    78781 gcgttagaaa cgatgcggtg ttccgcacga agttgatcct gattgacgcc gtaaacaacg
    78841 gtcgcgtcga gatcgttact gccaggatgt gaaaagagca cttttttggc cccggcggca
    78901 atatgcgctt cgccatgctc gcgggagcca tatacgccgg tgcagtcgag gactacatca
    78961 acgccaagtt cacgccaggg gagcgattgc agtgaacgtt catgcaatac gcggatggcg
    79021 tcatcaccaa caaaaagttg atcgcgttcc tgtcgtactt cccatgcaaa acggccatgg
    79081 ctggtgtcat atttcaacaa atgcgccatg cccgcagcat ccgccagttc gttgattgcc
    79141 accacggtaa tttccgcccg gcgtccggat tcatacaaag cacgaaccac attacgcccg
    79201 atgcgaccga agccatttat cgctacgcgt acggtcatag atctcctgca aggttttccc
    79261 tgagcaaatt tgccagacag agtaatccag caaatcgtcc ggggaaacct tacctgtcgc
    79321 aaactgcgac tgattggtta attgtcgaac atttaatcga ctgaaacgct tcagctagga
    79381 taagcgaaac gtggaataaa aggaatgatt gtccagtcaa agaaagcaat tatctgactt
    79441 acgtcacgtt ttcagccccg cccctgtgga atttattcca ctttgcagaa tcacgaataa
    79501 gcgtagcaaa atcttttcgt tgtgccgtca caaaccctga cttatatcag aatatgactg
    79561 atgcagcgtc gatattatcc gcaatacgtc cgagtttcgg agtactccaa tgatgacatt
    79621 gccctaccca tattcttcct ccgcacgctt ttacgcactg cgcctcctcc ccgggcagga
    79681 agttttttcc cagctacacg cttttgcaca gcagcatcaa cttcatgccg cctggattgc
    79741 aggctgtacc ggtagcctga ctgacgtcgc cttgcgctat gccgggcaag aaggcaccac
    79801 gctcctgaac ggtacattcg aagtgatctc actaaacggc acgctggaac agagtggtga
    79861 gcatcttcat ctctgcgttt ccgatccaca cggcacgatg cttggcggtc atatgatgcc
    79921 cggctgtacc gtgcggacca cgcttgaact ggtgatcggt agccttgagg aactggcgtt
    79981 tagccgccaa ccttgcgcgc tttctggtta cgacgagttg catatttccc ccgttaaata
    80041 actactttca aagaggtttt cttatggcgc gtagccactt ttccagtcag gcactggtgt
    80101 tgatcgttat atctatcgcc atcaatatga tcggcgggca actggccagc atggtcaaat
    80161 tacctatatt tctcgactcc atcggcacac ttattagcgc ggtactgctt ggtccggtga
    80221 tcggaatgct gaccggctta ctgaccaatt tactctgggg attgctgacc aacccgatcg
    80281 ccgccgcatt tgcacccgtc gcgatggtca tcggcctggt cgccggttgc ctggcgcgag
    80341 cgggttggtt tcgcaccctg ccaaaagtcg ttgtcagcgg cgtgattatt acgcttgcgg
    80401 taacggtcgt cgctgtaccg ctgcgtactg cgctgttcgg cggtgtcacc ggtagcggag
    80461 ccgatttgtt cgttgcctgg atgcactcga tggggcaaaa cctggtggag tcggtggcga
    80521 ttacggtgat aggcgcgaac ctggtcgata aaattcttac agcggtgatt gtctggcttt
    80581 tactgcgcca gctgccgatt cgcaccacgc gccattttcc ggcaatggct gccgtgcgct
    80641 aaatgcatcc atttacctca ttaacactgt gggcattggc ggcctgcacc acgcttatcc
    80701 tgcccgcgca gaccattttg ccgatctata gcgcggcgac ctttttctgc cttattgcgc
    80761 taaaggccac gcagcggcgg gcaaaatatg tcgtctggct gatgttctcg ctgggagccg
    80821 ggctgtggct ggtacacggt ggctggctac cagaatggct aagcggcacg ccacgctcgc
    80881 cagaacggtg gtcgcatgct attacgctct ggttgcggat tctggcgatt gtttcaacct
    80941 cacagttgtg gatgcagtac gtccccgtgc aatgttttat tcgcgctctg tttgcttctc
    81001 gcctgccgcc aggcgtcgct tatctgtttg ccgggccgtt attggtcgtt gagcagttaa
    81061 aacggcaatt agcgattatt catgaagcgc agcgtgcccg cggcgtgccg ctggatgagg
    81121 gctggtatca gcgtttacgt gcgatgcccg cgttaattat tccgctaaca cacaatgcgc
    81181 ttaacgatct ggctgtccgt ggtgcggcgc tggatatgcg ggcatttcgg atccacaacc
    81241 gacgaacaac gttgtgggca ccagccgata gcaccctaca acgcgtagcg cgttacacca
    81301 tgatcttgct gatgctgaca gaattcggag cgtggatatg gttacgttag aacagttccg
    81361 ttattgcccg acacactcaa cgcatcctcc gttctgttat gactttcact atgtcaaacc
    81421 ggggatggtc gccatctttg gtgacaacgg cagtggcaaa agtacgctgg cacagctgat
    81481 ggcgggctgg tatccggact ttctgccagg agagataaca ggcacgggga cgctcctcgg
    81541 tacgcccatt gggcatctcc cgcttaatga acaatccgcc accatccagc tcgtccagca
    81601 atcgccctat ttgcagctct ccggctgtac ctttagcgtg gaagaagaag ttgcctttgg
    81661 gccagaaaat ctgtgtcttg ctgaagcaga gattatggcg cggattgatg ccgccctgac
    81721 tctgaccgag tgccagccgt tgcgccatcg tcacccggca acactctccg gtggagagac
    81781 acagcgcgtg gtcatcgcct gcgccatcgc gatgcagcca aagctcttaa ttcttgatga
    81841 agcctttagc cgcctgactc cgcaggccag cgaaatgctg ttgcagcgct tacaacactg
    81901 ggcgtttgaa cgtggttcgc tgattattct ttttgaacgt catcgcacac ctttcctgaa
    81961 ctactgccag caagcgtggc aattgcaaaa cggagcgctc cagccactat gttaacgtta
    82021 aataagatat cttatcgctg gcctggcgcg gcaacggatt gtctttgcga catttctctg
    82081 caactcaaac aaggtgaatg gttggcgctg accggcgaca atggcgcggg aaaatcgacg
    82141 ctgttacgag taatggcagg tcttcttacg cccacagccg gtacggttat gctgcaacaa
    82201 caggcgatga aaaacctgaa aaaccgccag cgcgcggcaa aggttggcgt actgtttcag
    82261 gaagcggaaa accaactttt tcacagcacc gtcgctgacg agatcgcatt tggtttgaaa
    82321 ctgcaaaaat gcccggcgga tgaaatcact caacgtaccc atgctgccct gcaatgttgt
    82381 cagttagcag acactgccaa ctcgcacccg ttggatctgc atagtgcgca gcgacggatg
    82441 gtcgccgtcg cctgcctgga agcactctct ccgccactat tgctgctgga cgagccgagt
    82501 cgggattttg acgaaaattg gttgagcgtg tttgaaagct ggctggagaa gtgcggccag
    82561 cgaggcacga gcgtcgtagc aatcagtcat gacgccgcat ttacccggcg tcatttctct
    82621 cgtgtggtgc gactggaaga tggtctaatc aggaacgtta atccaccaga cgatattcac
    82681 ccgtagcggt catcattaag gtcaaatttg caggcagact ctcttccagc ccccggcgaa
    82741 cattcggccc gtcggtacgg tcataaaaag cttctgcgtc tgccagcgat acccctcccg
    82801 ccagcttgcg ccctaccagt cgttcccgca acgcagtagc tggcgcgttg ataaagatcg
    82861 agaaatcaca aaattgcgca agctggcacc acttctcgtc gtccagcaac aaccagtttc
    82921 cttcgacgat gacgagtggt gcggtaacgt gcaacgcatc ttcaacagga tcatgctttt
    82981 gtcgatcata ctgcggccac gtacaatccc cttccactac ctggcacaga ttttccgcca
    83041 gtttcgcaac gtcaaatgtc tctggtgcgc ctttgaaggg gcgcaattga tgcgcatcca
    83101 gccagctatt gtaatggtga aaaccatcca tcgggagcgt ctggatagcg ggtaattcag
    83161 ggtcttgttg cgcgagatat tcccagaagg tagttaaggt agatttaccc gtccccggcg
    83221 gagcgcaaag aaaaaccacc gtacgtcgct gcggacttac cgtctgtaag gccgccagca
    83281 tacgcagcaa cggtttatgc acgcgttcta tctcttcatc atggtactgc gcttgcacgt
    83341 tcagcccatt caccgttaat tcaattttca cggcgttaat tcctttaagg tggtcgttac
    83401 tgccagcgtc agcgccgttt tgaccattcc gctaaaacgc gcctctgacc agacggcgaa
    83461 atcggcgaac ttcatcgctt ttaacgcgtc atagagcgtc gcattgcagg ttatgctatt
    83521 gacgttgctg ataaaatccc aggtcatgtc atcggcaaat ccgggcgttt catcctgttc
    83581 gttcaggtaa tgccagtact gggaaaagtg cgtgatatcg gcatacagcc atttatccat
    83641 tttccccagc gcataaatca aacgcagcgc aacatcaata tcgtcaagcg ggccgctttg
    83701 ggcgagtaac ggcttcactg catattcgac gatctcttca tcgttatcga caaacagcga
    83761 cggcagaaac tggcgtatcc cctggagcaa aatagaatgc gcagttttca taaatgaaaa
    83821 taaattgtcc tgtgcatcca gttgctcaag cacatcatct tctgtcagcg tcgccattgg
    83881 tcttccgata gatcaccatc aatgcaggga gtctattata ttacacgttt ggtccgcgcc
    83941 gccgatcagt aatgtctgcg tccgcacccc attcgccgtc tgttggatac cgttcaccaa
    84001 ctcgccgccc gtcacgcccg tagcgctaaa gaggatatcg ttccccctca ccagttcatc
    84061 gagcgagtac acacggttaa cgtcaacgcc cattgcctta caacgcttgc gctcctgctc
    84121 tgcaatctgc cgattttccg tgtaatcccc tttagcctga caaaagtcaa tcagctcggc
    84181 ctgcatatcc ccacctaatg ctttaacggc acaggcagaa atcacgcctt ctggcgcacc
    84241 gccgatggtg tacatcacgt cgtaaggatt atcctgccag caggtcagca cgctggcggc
    84301 gacatcgcca tccggcagag cgaaaacctt cacgcccagc tgtgttgctt cttcaatcgc
    84361 ggcactcagg cgcggtttat ccagcgtaac catgcgcagc ttgtccagcg gtttacctaa
    84421 cgcccgcgcc acgttacgca agttgtccgc cagaggcaaa gacagatcaa tcgccccggc
    84481 agccagtcga ttaacaacca gctttttcat atacatatcg ggcgcatgta acaggctatc
    84541 tcgaggggcg aaagccatga ccgccagcgc attgctctgc cccatcgcta ccatccgcgt
    84601 gccttcaatc gggtcaaccg cgatatcgac ttctggccca tcgcctttgc ccacctcttc
    84661 gccaatccac agcatcggcg catggtcaat ttctccttcg ccgataacca ctcgtccacg
    84721 aaaagcgacg tcgtttaatg cctggcgcat cgcggtgacc gccaggccat caattttatt
    84781 tttgtcgcca catccggttt gcggccaggc agccaacgct gcctgttccg taacgcgaaa
    84841 taatggccac gccagggaca tcattgcgcg gcctccccga tatcaacgcc aaattgtgcc
    84901 agcagatatt tctcggcctg ttcattccag atgccgtgcg tttcctcaac cagccgcgcc
    84961 aactctttaa acagcggatc ggttttgcct ttttcggcaa aatcggcaat ggcagtgagc
    85021 ggcatggtca cgccgttata aatgagtttt ttaccgccag ggatatccgg cagattgagc
    85081 acggtatctg gcaccgcatc cagcccaccg atatgcgtga ccataaagga cggctgcaac
    85141 tgccccgtgg cgcttaacgc aatcgcctct ttcatgtcgt ccgttgaacc gccggatgta
    85201 ccgactacgt gcgtgctatt gtaatggacg ttgtagaaat taaacggcac tttgaagttt
    85261 ttatccgtcg gcccggcaaa gaagttcaga cagccatctt ccgccagtaa ttcgtcagcc
    85321 atctcaacga cagcaggcac cgccgcataa acaaaaatgt catcgaaccc gacatctcct
    85381 gtcagcgccc gcagcgtctg gaccggatcg ctcatccctt tggtattcac atacaccagc
    85441 tcaatgcctt tactggccgc cagatccacc ggcagcagct tctgcacctg cgccaggcgt
    85501 ttgtcgtcga tatcgaccac caccacccgt gacggttgta tgccaccgtt aatggcgtaa
    85561 tcgatagcgc caatgcccat cggacccgca cacgccagca gtgcaatatt gccgccaggt
    85621 ttgacgccca tgcgatgctc ataaacatat tgcgtggtgt gataattggc atgataagca
    85681 ccaataatgc agcacatagg ctctgccagc gacgccgcag caaaataaga gccgtgatac
    85741 ggtaatacgc agcccaaatt aatggcgatt tcgggaataa tcatataggt ggcgttaccg
    85801 ccaaaatatt cgtagctgta gcctgctgaa tatccgcttg gtaaccccat cgccggttgc
    85861 aatacaaaac gctggccttt tttatatttg ccagtgagat ttttacccac ttcgacaata
    85921 accccggcac attcatgccc ggtaatgacc ggatgatttt ctaaatcgtc gggtacgcgt
    85981 ttatgttcac taccgagtaa cgccgctttc caggtcgata aacagacgct gtcagaaatt
    86041 acactcacta acagttcatt atcggtaatt tctggcagtt caaattcgcg cagacggaca
    86101 tcccgcttgc cataaatagc agcaactttg gttttcattt ttacctctgt aatcagtgtt
    86161 tatgttcggc ggttaattga ttaaataaag tgtcgagttt tggatcgcca atatagttat
    86221 taatcaatat cagcggttta tccgtcacac gtttcacgcg cccttccaga ctggcgtgag
    86281 tgacgacgat atcggcatcc gcaggcacgt tttctatcgc gtaatgtttt acttctattg
    86341 ccagccccgc tttttccagg cgtttacgga aggtggtcgc gcccatcgca ctggagccca
    86401 tccccgcatc gcaaacaaag gcgatacgct taaccctgct taacgagaat gcaccctctt
    86461 gtttcatcgc cttaaccgca ttggctgact gagcaaactc atcttcgctc tccgtttcca
    86521 ccgttttttc catcttcagt atcagcgaag taatagcaaa ggacaccagg gtacctaccg
    86581 taacaccggc aattgtcgcc aggaacgagc ctttcggcgt cagtgccagg taagcaaaga
    86641 tagaccccgg acttgggcca gccaccagac caccgtccag taagttaaac atccaggtac
    86701 cagacatacc gcccgcaatc atggcaataa tagtcagcgg cttcatcagc acatatggga
    86761 aatacagttc gtggatccca ccgaggaagt gaataatcat cgccccgggc gcagaacgtt
    86821 tactcatccc tttaccaaac aaggtaaacg ccagcagcag ccccaggccc ggacctgggt
    86881 tagaggccac cataaagaag atggatttac cgttaaccga agcctgttgc attcccagcg
    86941 gataatagac gccctgatcg atcgcattat taaggaaaag cactttcgcc ggttcattga
    87001 tgactgacag cagaggcaga taacccgcat gtaccagcgc ctcgatacac tctttgacga
    87061 aagtattggc aattaacacc gccgggccga tcacttcaaa acccagcaga caaaggagca
    87121 tccccgcgat acctaatgag aagttattga taaccatctc aaaaccggca ggtatgcgtt
    87181 tttccagtgc cttatcgaca tattttatga ccaacccacc gagcggcccc atgatcattg
    87241 agccaaggaa catcgggatc tctgcgccaa cgatcacacc aatagtacct attccgccca
    87301 tgaccgcccc gcgtttaccg ccgaccagat gaccacctgt agaaccaatc atcacgggca
    87361 ataaataggt aatcatcggg ccgacaattt tggcgaaatg ttcgttaggc agccaaccgg
    87421 tgggaataaa taacgcagta ataaaacccc aggcaataaa agcaccaata ttggggatga
    87481 ccattgcagt caaaaagccc ccaaaagcct ggacctttgc acgagcagac ttgttttcca
    87541 taatattatc ctgtagagga gagagtaatt atccgcggct gataatgtcc gctaattgtt
    87601 tttctgatga tgctgttaag agttgttcga gtatttcttc ttcgcacagt aattcactta
    87661 acgcctgaat agcgccaatg tgcgaatcgg catcggcagc agagagtccg atgagtaatt
    87721 taattggttc gtcattcccc ggaaaataaa caccttgttc aagtaatgtc aaagacatcc
    87781 cggttttaag cgccccacat tccggtcgcg catgaggcat tgccacgcct ggtgcgagaa
    87841 tataataagg gccattgtta atggtggaat ctttaattgc ctgaatgtaa ttctcgctga
    87901 tatagttttt atccagcaat gataccatcg agaaatcgat agcttcctgc caatcttttg
    87961 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
    88021 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
    88081 aaagcaattg agcagggaaa ccgatgtttt gaaaacagtg aattattaat ttcttatata
    88141 acattgagtt atagatataa caacaaatgt caatacgcat atcgtgatgc atattacata
    88201 attgatattt attgcatata aatatttgtg atctacaaca cgctttatct attgcttgtc
    88261 cgcaaacgga catatcaagg taataaaaaa ggtcgccgaa gcgacctttt tacccgaaat
    88321 gctaattaca gcagttcttt tgctttcgca acaacgttat caacagtgaa gccgaactct
    88381 tcaaacagca gctctgccgg agcagattca ccgaaggtgg tcataccgac gatagcaccg
    88441 ttcaggccaa catacttgta ccagtagtca gcaatacccg cttctacagc aacgcgtgca
    88501 gtaaccgctt tcggcagtac ggattcacgg taagcagcat cctgcttgtc aaatgcgtcg
    88561 gtagacggca tggacaccac gcgcgctttc acgccttcgg cagtcagttt ttcgtaggca
    88621 gcaacagcca gttcaacttc tgaaccggta gcgatgaaaa tcagttccgg ctgaccggcg
    88681 cagtctttca gcacataacc accgcgcgcg atgtttgcca gttgctcttc agttcgttcc
    88741 tgctgcgcca ggttctgacg ggagaggatc agtgcggtcg ggccgtcctg acgctcaaca
    88801 ccgtatttcc acgcgaccgc ggattcaacc tggtcacacg gacgccatgt agacatgttc
    88861 ggggttacgc gcagagaagc gacctgctct accggctggt gagtcgggcc atcttcgccc
    88921 agaccgatgg agtcgtgggt gtaaaccatc acctgacgct gtttcatcag cgcagccata
    88981 cgtacggcgt tacgtgcgta ttccacgaac atcaggaagg tggaggtgta cggcaggaaa
    89041 ccaccgtgca gggagatacc gttagcaatc gcggtcatac cgaactcgcg aacaccgtag
    89101 tggatgtagt tacccgcagc atcttcgttg attgctttag aaccagacca cagggtcagg
    89161 ttagacggtg ccaggtcagc agaaccgccg aggaattccg gcaacagcgg accgaaagct
    89221 tcgatagcat tctgagacgc tttacggctg gcgattttcg ccgggttagc ctgcagttta
    89281 gcaatgaact cttttgcttt cgcgtcgaag tcagacggca tttcgccttt catacggcgg
    89341 gtaaattcag cggcttcctg cggataagct ttcgcgtaag cagcgaattt ctcgttccat
    89401 gcggattctt tcgcctggcc tgcttctttc gcatcccact gagcatagat ttcagacggg
    89461 atttcgaacg gtgcatattt ccagcccagc tgttcgcggg tcagggcaat ttcagcgtcg
    89521 cccagcggcg caccgtggga gtcgtgagta ccggctttgt tcggggaacc gaaaccgatg
    89581 atggttttgc acatcagcag ggacggtttg tcagtcactg cgcgcgcttc ttctactgcg
    89641 cgtttgatgg atgccgcgtc atgaccgtcg atgtcgcgaa taacgtgcca gccgtaagct
    89701 tcgaaacgca ttgcggtgtc gtcggtgaac cagccttcaa cgtgaccatc aatagagata
    89761 ccgttgtcat cgtagaacgc aatcagttta cccagcttca gcgtacccgc cagagagcaa
    89821 acttcgtggg agatgccttc catcatgcag ccgtcgccca tgaaggcgta ggtgtagtgg
    89881 tcgacaatgt cgtggcccgg acggttaaac tgcgccgcca gcgttttttc tgcaatcgcc
    89941 atacctactg cgttggcaat accctgcccc agcggaccgg tggtggtttc cacaccagcg
    90001 gtgtaaccca cttccgggtg gcccggagtt ttagagtgca gctgacggaa gtttttcagt
    90061 tcttccatcg gcagatcgta accggtgagg tgcagcaggc tgtagatcag catggagccg
    90121 tggccgttgg acagcacgaa gcggtcacgg tcagcccagg acggattctg cgggttgtgt
    90181 ttcaggaaat cacgccacag gacttcggca atgtcagcca tacccatagg ggcacccggg
    90241 tgaccggatt tggctttctg tactgcgtcc atgctcagcg cacgaatagc attggcaagc
    90301 tctttacgtg aggacatttt gactccagat cggatgatga agggcacgcc cttaacgact
    90361 tgacgacagc gcgttttggg ctacgccgga aaatttgcca acaatttacc gcaagccgcg
    90421 cgtcatgtac atggaacatc cttttgccgc ttcagaaatc tctggatcat gctcgcatgt
    90481 tgcgcaatct actcgcccgt ccgctgcgct tttccttata ctgagactga gcgtcgattc
    90541 acctgcaaac ggcgcatttt tagaataatc ctgaccttgt gcggaagaga aaacatgaaa
    90601 attcgcgcct tattggtagc aatgagcgtg gcaacggtac tgactggttg ccagaatatg
    90661 gactccaacg gactgctctc atcaggagcg gaagcttttc aggcttacag tttgagtgat
    90721 gcgcaggtga aaaccctgag cgatcaggca tgtcaggaga tggacagcaa ggcgacgatt
    90781 gcgccagcca atagcgaata cgctaaacgt ctgacaacta ttgccaatgc gctaggcaac
    90841 aatatcaacg gtcagccggt gaattacaaa gtgtatatgg cgaaggatgt gaacgccttt
    90901 gcaatggcta acggctgtat ccgcgtctat agcgggctga tggatatgat gacggataac
    90961 gaagtcgaag cggtgatcgg tcacgaaatg gggcacgtgg cgttaggcca tgtgaaaaaa
    91021 ggaatgcagg tggcacttgg tacaaatgcc gtgcgagtag ctgcggcctc tgcgggcggg
    91081 attgtcggaa gtttatctca atcacaactt ggtgatctgg gcgagaaatt agtcaattcg
    91141 caattctccc agcgccagga agcagaagcc gatgattatt cttacgatct tctgcgccaa
    91201 cgcggcatca gcccggcagg tcttgccacc agctttgaaa aactggcaaa actggaagaa
    91261 ggtcgccaaa gctcaatgtt tgacgaccat cctgcatccg ccgaacgcgc ccagcatatt
    91321 cgcgatcgta tgagcgcgga tggggttaag taagtcagac ataccgatct acaacataat
    91381 tgtaggtcgg taacgcctga atccccgatc agagtagtga tttcacttta tttagcaaaa
    91441 cggctacatc atccggtcgg gtatatcccg tatcagggtc tataatcgag ctgtaaacat
    91501 gtggcataat gcgcggcacc cccgcctcca gacaagtttt cagaatcaca ctgaagttat
    91561 ccaaatcaat cccgccagtc ggctcaatca gcgtcatacc attgcgcgcc gccgtggtcg
    91621 ccagcgcgta tagctccgga agcgacctct cgccgcccat cggaaagaac ttcgccgcgt
    91681 gtgcgcccat atctagcatc attcgcaccg ccgtatcgca ggagacgcgg gcaggcgtac
    91741 cctggctgct gctaacgccg gtggaaatca gcacttcgcc cggcgtcccc gtcgggctaa
    91801 ccagcgcgtt gacatgggtc tgctcaccgc ccgtagccgc aagcgcgcct gccgcgaaac
    91861 cacaaccggt aaaggtctgg ttaacgtgtg ctggatgaat ctgcgccgca atcatcgcag
    91921 ctttgtaata ctgcgccggg tcacccgccc ccagacctac tgaaatggac ggtatttccg
    91981 ccatccaacg tttaacttcc tggatcccct catccaccga ggagaactgc gcggaaagca
    92041 cgccaatcac tgcgtggcct tcggcggcgt caaagatttc acgggcattg gcgatatctt
    92101 tcgccagcac attaattgcc acgcgattac gatagaaatt aatcttttgc atgatctgca
    92161 atctccttca gtcgcgccac gataagtgcc atttcccctt ccgccaccgt acgcggatca
    92221 aggctaaaaa cgccctggtg gaggttatac cgccgtgcat aaatcgcgat atcgccacca
    92281 cgcagttgcg cttcgacgac gcgagcgtca actcccaatt cctgcgcatt tactcgaata
    92341 cgaatacgcc agattgcacg cccggcttca tcctgctcaa tatcagccgt taacccgtga
    92401 atcgcagaaa tcgcttcaac cactggctgg agctgttccg cggtgatgac tgcctgcccc
    92461 tggtgataat tctccagcgc gtaaaccagc ccaaccatgt tctccttgcc gattttcatc
    92521 gccctggcaa tgccgtgatg ctgcgctttg caggctgcaa tccagatttt tttaccagtg
    92581 ataaacccgg aggtcggcgc gttaaaggct tttgcgccgc tgtagaccac catatccgct
    92641 ccgctcacca cccaggcacg taaatcctct tcggcggcgg catcgacaat cagcggcagg
    92701 ttgtacatct gcgcgacatg tgcgaagtcc tcgatgttca acattccctt ctgcacgcag
    92761 tgatgcgact tcacatacag cagcgcggcg gtcgcgtcgg taatggcact ctccagctgc
    92821 cagagagccg ccaggttact tgaacccacc tccacaacgc gcccgccacc taagcggatc
    92881 gcgctgagga tcgatgcacc gtaatcgacg ttatggccgc gcagcatcac cacttcattg
    92941 gccatgccgg agctatccgg catgagcgcc acccgggcct tgtcgccgcg tgtaatggcg
    93001 gcggcgacag cgatggcgat ccctgctgag gcacaggagg taacgtagct atcctccgcc
    93061 ccggtaaagc gagagaccag ctcgccggtt ttgtccacca gctggtcaat ctccacgaaa
    93121 gaggatgccg cgcgcgcggt ggcctgcatc acctccggcg cgacgctgga gacgccgaga
    93181 atcgtcatct tgccgcaggc gttaatcacc tgtttcagtc ccagttgttg atagatattc
    93241 tgcgtcatgg cttacagcac tcccagcagc gagcagacca cgctcaacgc cacaatcgac
    93301 agcagaatcg tggtgtaacg cggacccttt ttcaccaaat agaagtagat ggcgaacact
    93361 gccgccagcg gcagcaggcc gggcgcgatg gagtccagaa tctgctgtac caccacttca
    93421 gagcctttca gtgcgctaat ttttaaaggt gaggttatct tgacaaagct cgcggatagc
    93481 gcgcccatca tgataagccc cagtacgtta gcgccgtaga taagctcctt gatgcgccct
    93541 ccctgcagca gactgataat cgaatcgcgc cccagcgtat agcccttgtg caccatgccg
    93601 tagctgattg ccagcgtaat cgccgggtag agaatcagcg gcaaaatacc gcccatcgcg
    93661 ctgccattgg cggcgaacgg gataaaaata gcaatcaaca gcggcatcac cgccgcccag
    93721 atgatggagt cgcccatccc ggccagcggc cccatcaggc cggttttaat cccggtgata
    93781 gaggcatcgt taatcgcctc gccgcgggtc ttttgctctt ccatggcgat ggagatcccc
    93841 tggatcaccg cgccaaacgt ctgctctgag ttgaagaagt tcagatggcg ttttaacgcc
    93901 tccacctgct cctccttttg cggatagagc tttttgataa tcggcgtcat cgaggcgcag
    93961 aaaatcaggc tctgtaaacg ctcataggag ctggaaactt ccgcgcccag ccagtagata
    94021 aaccacgctt tggtgatatc cgctttggtc agcgccccgg actcccgcgc gcgctcaacc
    94081 agttcatgct gcattacgtc gtcgctcatt atgccgctcc ttcatttttc gccaggcctt
    94141 taatcaggaa ggccacgcag gtgccgaaaa tcgccatcgc catgatgtcc accttgaggt
    94201 acagcaccgc gaagaatccg ccgataaacc acggcagcag gctcttttta ccaatcacca
    94261 taatggtgat ggcgaagccc agcgccggga gaatcccgcc catgatttca aacgagtggg
    94321 tcagccagtg cggcatcagc ttgaggaaac tttccactac gttctggccg aagtagttgg
    94381 cggcgaacac cactgggaaa cgtagtacca gccccagcag cgccgggtag agaaaggcgc
    94441 agcgcataat ggctgacata ttggccgttt ccgcgtgttt atcggccata tgcacccagg
    94501 ccgcatttag cgtgcgacgc aactggtcga ggaatacgcc gataacgcca aatggaatcg
    94561 ccagtgcaat cgccaggttc gggtccatat tggccttaac ggcgatagga atggcgatac
    94621 aggccgccag cgccgggtct gacggcacat tgccgcctgg cgttgaggtc acacccagat
    94681 agaccaactg catccccgcg ccgataatca tcgccgcagg catattgccg agcagcaggc
    94741 cgacaaagac cgccaccacc actggctgta acagcatggc ggagaaggta tagccaaggc
    94801 gcaaccgggc aaaccagtaa tacagcccca tcaggcttgc aaaaagtaag gtatccataa
    94861 tttgttatcc catgttcaga gggtcagaat tttttcagga tatcgtccag cgactgcggt
    94921 ttatcttccg ggatggtctg gaaaacagcc ttaatgccgc gacttttcaa atcgttaaga
    94981 atgccaacgt ctttttcatc cagcgttatg ttctggaaca ccgctttacg gtttggcccg
    95041 ccgcctaaac cgccaacctg aatatcagcc acgtcaaagc cctgctgcac cgcttcctga
    95101 atcgccgcca gcgacgggaa cagcaccagc acgttgccgt cgcccagctg gttctctttc
    95161 cacgcggccg cgaagctctg gttgctaaag cagtccactt tgatattcgg cggcgcggcc
    95221 atcaggtaga tatttttcat aaacggatcg gcgtccagct cgtcgctgac caccgcaata
    95281 cggttggcct gagactgccc tacccacttc gtcaccacct gtccgtggat aagacggctg
    95341 tcaatgcgac ataacacgat gtttgccata cgattttcct tactgttgta attgacgaat
    95401 atgggagacg acgtccaggc aactgttgcg cccagcttcc accagatcgg ccacgcaggc
    95461 ggtcagtggg ccatgttcgc gcttgtccag cgcctccagc agcagcgagg cattaagccc
    95521 ggagatcacc gccaccggat aatcggcgct cagccgcgcc gccacgttag aggtggtgcc
    95581 gccgataaaa tcggtcacga taagcgaacc ttccggcatg gttttcacca cggcttcgac
    95641 gcgctggtaa aactcgccca gcgtatcgac gggcatcagc gcgatttccg tcacgccttc
    95701 gatttcgccc atcaccatgc gcagactgtt gcagagctgc agcccccagc cgccgtgagt
    95761 tagcagcaga atttcaggta acggatgaat aggtacagtt gtggtcaaaa tagcctcctt
    95821 tgctccagtg tttacatttc atcaggagca aaggacgtgc cagaatttgt gtcacgcaca
    95881 gaggaaatga gattggatgg ggcgttaagc cccgtaaacc aggggatcag ctgtagagca
    95941 gttcgtagat ataaaaatat tctgcgtccg ataaacggat agcgtaagcc tcttcaatcg
    96001 gcaaaaagga ggatttgatg acgctaaagg cacgagcgtc cagatcaagc cggttttcta
    96061 gcgccatctg taacggtttg cggttaatca cgatacgctc gaccatgcag cagcagtgga
    96121 tcagaaaacg tagtgtcacc tggcggctcg gtttcaggga gagcgtgttg gttaagtagt
    96181 tcagaacatc ctcgatctct ttcagaattc gctgcgggtt gagcaccgaa atatggttga
    96241 tgatgctctc catggtcagc gcgctgataa agcgcatggc gctgcgctcc atctccagtc
    96301 ggcgctcgct gcttgagaga tccggcgtca gcaggctgag caccagctcc ggcccttgct
    96361 cggaaaacag ttcctccagc gagataaacg gtatatccgg cagtcccggc tggaaggtgc
    96421 cgacgatccc tgccagccgt tcactggcat taagcgcctg ctgaatacgc tccaggctgc
    96481 gcacctcgtt atagtcgaga atcaccatac gcgtgtcctg cgacatcagc tcgccaaagc
    96541 tctcctccag cacctttttg atcttttccg ccgtccccat tccggttata catgagataa
    96601 ccagcacttt gccaccgctc tcttgctgcg gcgtgcagag ccgacaaggg atatttttac
    96661 tctgcataag cacagccaat tgcgatagat cgccagtttc gtagcttaaa tccagcccaa
    96721 cctccagcag gctggtcaga gtgatatttg gcaacaacag aacgtcaatc tggaacagct
    96781 tgctgatggt gctgccaaaa tggaccagcg atccgatatc caccatcagt atcagccgct
    96841 gccagcaccg gtcctgaatc atccgggtca gggtttccag ggtgtcatgc accgactgct
    96901 caaatggcat atcaattgcg tagaacagat cgcgctccag cacccggttc acatattgcg
    96961 cctggctcgt tgcggtggtc gcaccgtgag cgattagaat cacgccgcaa tccgggctgg
    97021 tgtcgatgcg ctggcggtag tggcgacact ctttcaggaa caggcacagc cagaccacct
    97081 ccgtcgccgg gcactgaata tgcaacaact cattgatttt gcggcacaga agcgtggcat
    97141 tgtcatactc atctttacag cgatccagaa tcaggctgga tgaatagagt tgaggtataa
    97201 gccctcgccg cacatagcca atcagcgcaa gaaagtgttt acgtaacggg ttcgccaggt
    97261 tttctggcaa tgtaaatcct aatacctgct ccacgtaacc gatcagcagc gtcacacgct
    97321 cctcaatttg atcgccatag cgcggtggat gcgccgcact atggcggcta taaagaccat
    97381 attcaaaaat cgaactgagt ttgtttttca ggatagccag cgtttctgcg ggcggcacgt
    97441 tactgttgcg cagattgaca tattcacgag tcaggaaact gtaaaacagg tcgctctctt
    97501 ctatttccgc gctggtcgcc aacgaatttt ttaacgcggg caacgtacgc gcatccacgt
    97561 tcagacgatc tttaccgcca aacaacgcat cgaccagcag acgctgctct ggcgtggtat
    97621 taaacgacat ctccgccagt cgtttatcta actctagcgt atcattacgc tcggtcattc
    97681 ccgacgccca cgcctgcgcg cacaagaact gaatatcact tttaagctgg ccgatattgc
    97741 cttccagggg tttacttaac aaccaatgca agagcgtttt atcaaggctt atggtacgtt
    97801 caattttacg actttcacgc tgaaagaagc ttacaatcaa ttcgagctgc tcctcgaccg
    97861 aacgctggcg aatacccggt aagtcaatac atacctggat acgccgctgg aaggtacgca
    97921 atagtgccga gttgaccgac tctgtagtgg cgcaaataag gcgcaccgaa attgaatatg
    97981 ttggcccact tgaaccaagt gggcggtatt cacctttatc gagaatagaa aagagcttct
    98041 cttgtccttc gtacgggagg cgatgcactt catccagaag taaataacct ccgtcagctt
    98101 gctccaccaa tcccgtttta ttctcacttg ccccggtaaa tgccccctta tggtgaccaa
    98161 acagatgtga ggaaagcaac tccggattat gagcatattc ggcacagtta aagtaaacca
    98221 acggaggtgg cgaacctatg gtgtgcttgc aagcgaagca atgcattaac tcagcaaaga
    98281 atgttttgcc aacaccagat gcaccggtta acagcacatg tagtccgtgg ggataaagca
    98341 ccgctgcgcg gcctttttct accgcatcac ggagactacg gtcgtatccg ataagtccgg
    98401 aaaacggatc atcatctgta tatttttctt tctttggcaa taaactagcc actgaatgaa
    98461 ctatgcattc gcaatcatcc agttttcgat taatgagcaa ttctagtgcg tgacgatgta
    98521 gaaaaaaaac aggacgtccc tgacttttta ccaccaggcc ttcattccat aattgattaa
    98581 gatctttact aaccgagttg cgtgccagcc caaggttaaa accaatcgcc tcagcagtaa
    98641 atgctttctc cttcgctaaa tcagacagtt ccagcccata cgtcagccgc tccagctcct
    98701 tgtgaataac atcaatccgt ctcatttctt taccgcttaa aaaacaatag ttgttcagaa
    98761 catacactat ttgtaaacgt aagaatgaga actatcggga agaattaatg ttccttgtgg
    98821 caatctggtt tctgaatatt cgtgttttaa atattactta tggtctgaaa caaccaggca
    98881 ggtgaacaga gctaagaaaa agtagcgtag aaatataaaa acatatatcg cactgatgaa
    98941 tgtttcccgg catgactatg cacaccggga aatatttagt aaattactcg ccctttttcg
    99001 ccgcctgaat atacagcatc tccagcgcca gcgtcgccgc agccagagca gtgatttccg
    99061 actgatcgta tgccggagcc acttccacta cgtccatccc aacgatgttg agatctttca
    99121 ggccgcgtac cagtttaatg gcgcgatcgg aggtcagacc gccaattacc ggcgtaccgg
    99181 ttcccggcgc aaatgcagga tccaggcagt cgatatcaaa ggtcaggtaa accggcatat
    99241 cacccacaat ttgtttcacc tgggcgataa cgtcatccac gctgcgatcg ttcacctggc
    99301 aggcgtccag cacggtaaag ccgttgtctt tatcaaattc ggtacgaata ccaatctgca
    99361 cggaatgatt cggatcgatc agaccttctt tcggcgcggt atagaacatg gtgccgtggt
    99421 caaattcaca accgttcgcg taggtatcgg tgtgggcgtc aaagtgtacc agcgccattt
    99481 tgccgaaatg cttcgcatga gcacgcagca gcggcagcgt aacaaagtgg tcaccaccga
    99541 aggagagcat acgcttaccg gcagccagca gcttctcggc gtgcgcctgc agcttctcgc
    99601 tcatctcacg ggcatcgccg aaagcatata ccagatcacc gcagtccacg acgttcagac
    99661 gctcacgcat gtcgaaattc cacgggaagc ggttgtgttc ccaggccaga ttcgtcgaaa
    99721 cctgacggat agctgccgga ccgtggcgtc cccccgcacg accagaagtg gccatatcga
    99781 acggcacacc agtaatcacc cagtcagcat cgctgtcata cggctggaag ttcatcggca
    99841 ggcgtaaaaa accaaaggca ttggaaacca gtgagttatc gtattgatga cctaaggtgc
    99901 tcatggatgg actcctatta caaagtcgat ataaaaaaac ccttccgcgt cgttaggccc
    99961 gacgaggaag ggttggattt gcgaaacaca agtttgtgct aattatcgcc ttaaatgggc
   100021 gaggcatcaa gcgaggttgc tatttccgac acggtggcgg cgcatagcca ttgttatctg
   100081 ccggaaaggc ttgtccagag cacggcggca gcgcgttttg cgcctggcct tgtaattcaa
   100141 aggcggcaaa gcctattaca ccggtattac gcacatcgcc ttgcgccgaa ttggctgcgt
   100201 aggaatcagc aacgttggcg aactgaaaag ccgcttcggt atgtttatct ttgcggaatc
   100261 ctttaatcgc taaagaatct ccggcattca caatatagcc gttgttattc agcgaacctt
   100321 gcttgccgtt cagcacatcc aggccatcaa cagtagcaac gatctcgtag ttggtgttac
   100381 gactgtaatt acggacatat aattgataat tcattccgac tctggcatgg acaatatagc
   100441 gtggttcatt attggatgaa tcataaacac gcgtaatagg tagagaatta aaattaccat
   100501 cgcgaatagc atattccagt tcgttaatac gaatgctata aacgcggtca tagccggaag
   100561 gaacattagt cgaataacta ataacgatca tctctgccgg atcgcgccag gctcgctcag
   100621 cagatacgcc ctgaaccgat gagtgcacat ccccacccca ggcagtgccg acattgcccg
   100681 atgcactgct attatactgc gcattactgg aacaaccggc cagcagcacg attaatgcca
   100741 ccaacgcgct acgtactttc cattttttca taaccgttcc ctctgcattt aattaatgca
   100801 gcgattgtag ggagattatt taataagaaa agcagcgaga ttatttttaa gaatatataa
   100861 aaagaaagcg ggccgattta cgtaaataaa tcgacccgcc agcggaataa tcttaattat
   100921 tcatcttcaa gataggtata accgtacaaa cctgcctcga actcttcaag gaactgttgt
   100981 tgcagttcag catcaagatc ggttttcttc acttgatcgc ggaactgggt taacagcgtt
   101041 ttcggatcga gctgtacata ttgcagcatg tccgccacgg tatcgccttc gtcagacagt
   101101 tctacttcta cgctaccgtc agggaacacg aacacgtcaa ccgcttcggt atcaccgaac
   101161 aggttgtgca tattgccaag gatctcctga tatgcgccga ccataaagaa accgagcatc
   101221 ggcggattct ctggatcgta ctccggcatt ggcattgtcg tggcaatacc atcaccatca
   101281 atatagtggt cgatagcacc gtcagagtca caggtaatat ccagcagcac tgcgcgacgt
   101341 tccggcactt gatccagccc ttccagcggc agaaccggga acaactggtc gatcccccat
   101401 gcatccggca ttgactggaa cagcgagaaa ttgacgtaca ttttgtccgc catacgttcc
   101461 tgcagttcgt cgattatcgg acgatgcgca cggttttgcg gatccagctg cttttgcact
   101521 tcatggcaca tgctcagata gagttgttct gcccatgcac gttcttgcag gctaaaggtg
   101581 ccggaagagt agccgatatg aatgtcgtgc agatccatct ggctgtcgtg taaccattca
   101641 cgcagagaac ggcgagttcc cggttcgtgc atttcctgcc aggtttccca catgctttgc
   101701 agcgcgcgcg gcgcatcttc tgcaggcgcg gtcggcaccg tgtattcgtt acgttccacg
   101761 ccgatgatat tagataccag cacggtgtga tgcgcagtca ccgcacgacc cgattcggtg
   101821 attaccgtcg gatgcggcag accgttttct tcacacgcat caccaatcgc ccagatgatg
   101881 ttgttggcat attcattgag gccgtagttc accgaacagt cggactgcga acgggtgcct
   101941 tcatagtcca cgcccagacc gccgccgacg tcgaagcact gaatattgac gcccagcttg
   102001 tgcagttcca catagaaacg cgcggattca cgaacgcctg tcgcgatatc gcgaatattc
   102061 gccatctgcg aaccgaggtg gaagtgcagc agttgcaggc tgtcgagacg cccggcttca
   102121 cgcagggttt caacaagttg cagtacctga gtcgcagcca ggccgaactt cgatttttcc
   102181 ccgccggagg actgccattt acccgagccc tgcgaagcca gacgtgcacg cacgcccaga
   102241 cgaggaacga cattcagacg ttctgcttca tccagcacaa tggcgatttc tgacatcttc
   102301 tcaatgacca ggtagacctt gtggcccatc ttctcgccaa ttaatgccag gcggatatat
   102361 tcgcggtctt tataaccgtt gcagacgatg acgctacggg tcatgccagc atgtgccagc
   102421 accgccatca actctgcttt cgagcctgct tccagtccca gcggttcgcc cgaatgaatc
   102481 agggactcaa tcacgcggcg gtgctggtta actttgatcg gataaacaag gaagtaatcg
   102541 ccgttatagc cgtaggattc ccgcgcacgt ttgaacgcgg cgttaatgga acgcaaacgg
   102601 tgctgcagga tctgtgggaa acagaacagt gcaggcagac gctgaccctg tgcttcacga
   102661 gttttcacta actgcgcgag atcgacgcga gcttccggga cgtccgggtc cgggcacacg
   102721 ctgatgtggc ccagctcgtt aacgtcatag tagttattgc cccaccaggc aatattgtaa
   102781 gtacgcagca tcttgctggc ttcctgggag ctcattgcaa cctcctgcat ggagcgtagt
   102841 acaccgtgtt cgcccgctga cgaaggcaaa cccatagaca tgtcgtcaga catagcgaac
   102901 ctcaaattat tttattaagt gtaaaacagt taacgactat cgcagcctgg aactgcgata
   102961 acaacccata agcacatgga ttttccagca gtgtatgctg acgctccgac tgcgcaaccg
   103021 gtttcttttt cataacatta ttaagcacat aaccgaacgt aagtgtgaca gttcggcgaa
   103081 accacgagaa aactcttgtt tttacaagag cgcccttgtt cagtcctcag taactgtaac
   103141 cagctcttga atcctgagaa gcgccgagat gggtataaca tcggcaggta tgcaaagcag
   103201 agatgcagag tgcggggaac gaatcttcac cagaacggtg agacaggtta agcagcagac
   103261 aacggttcat tatttcgtat cacctccacg gccgcctgtt aagacgaacc cacaagccaa
   103321 aactctgatt tcaacccggc tggaagtggc aacacgaaag aaacgtcgtg tgctttttat
   103381 ttaagccgcg cgccgcgttt tataccccac aatggcagaa aattgcaaaa gataaatacg
   103441 cagaatgccg gcattgtcag gaaaaatttc cagccacgtt tttaacagaa tgagacacga
   103501 ttcaaaaaaa agtggaaatc gggtgaagaa ttgacctaaa atagccatcc agatgttaat
   103561 ccatccatac cgattaacac tcagactgcc agtgttttta acttgcagag tcgtggtagg
   103621 atccgctacc acagaaaatc cacacaacag tttgagctaa ccaaattctc tttaggtgat
   103681 attaaatatg gcaaaacacc tttttacgtc cgagtccgtc tctgaagggc atcctgacaa
   103741 aattgctgac caaatttctg atgccgtttt agacgcgatc ctcgaacagg atccgaaagc
   103801 acgcgttgct tgcgaaacct acgtaaaaac cggcatggtt ttagttggcg gcgaaatcac
   103861 cactagcgcc tgggtagaca tcgaagagat cacccgtaac accgttcgcg aaattggcta
   103921 tgtacattcc gacatgggct ttgacgctaa ctcctgtgcg gttctgagcg ctatcggcaa
   103981 acagtctcct gacatcaacc agggcgttga ccgtgccgat ccgctggaac agggcgcggg
   104041 tgaccagggt ctgatgtttg gctacgcaac taatgaaacc gacgtgctga tgccagcacc
   104101 tatcacctat gcacaccgtc tggtacagcg tcaggctgaa gtgcgtaaaa acggcactct
   104161 gccgtggctg cgcccggacg cgaaaagcca ggtgactttc cagtatgacg acggcaaaat
   104221 cgttggtatc gatgctgtcg tgctttccac tcagcactct gaagagatcg accagaaatc
   104281 gctgcaagaa gcggtaatgg aagagatcat caagccgatt ctgcccgctg aatggctgac
   104341 ttctgccacc aaattcttca tcaacccgac cggtcgtttt gttatcggtg gcccgatggg
   104401 tgactgcggt ctgactggtc gtaaaattat cgttgatacc tacggcggca tggcacgtca
   104461 cggtggcgga gcattctctg gtaaagatcc atcaaaagtg gaccgttccg ctgcgtacgc
   104521 agcacgttat gttgcaaaaa acatcgttgc tgctggcctg gccgatcgtt gtgaaattca
   104581 ggtttcctac gcaatcggcg tggctgaacc gacctccatc atggtagaaa ctttcggtac
   104641 cgagaaagtg ccttctgaac aactgactct gctggtacgt gagttcttcg acctgcgccc
   104701 atacggtctg attcagatgc tggatctgct gcacccgatc tacaaagaaa ccgcagcata
   104761 cggtcacttt ggtcgtgaac atttcccgtg ggaaaaaacc gacaaagcgc agctgctgcg
   104821 cgatgctgcc ggtctgaagt aatctttctt cacctgcttt caaaggccag cctcgcgctg
   104881 gcctttttct tttacctcac cgcggtggat aggcattcac gccgcatccg gcaaaaagct
   104941 gcccgcacaa aaacatcatt cttcctcaac acgttttatc gcaaattatc atcattactg
   105001 aaaccgatta caccaataac accagccaaa tatttgtaat attttcatat tattattcag
   105061 ttttcacagt tgttacattt cttttcagta aagtcttaat tgcagataac agcgtttaat
   105121 ctatgatg
//
LOCUS       DAZFWL020000003        87357 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFWL020000003 DAZFWL020000000
VERSION     DAZFWL020000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 87357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 87357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.003"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..596)
                     /locus_tag="ACHKNP_RS01055"
                     /old_locus_tag="pgaptmp_000211"
     CDS             complement(<1..596)
                     /locus_tag="ACHKNP_RS01055"
                     /old_locus_tag="pgaptmp_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU4702456.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHKNP_RS01060"
                     /old_locus_tag="pgaptmp_000212"
     CDS             complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHKNP_RS01060"
                     /old_locus_tag="pgaptmp_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGU4702457.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHKNP_RS01065"
                     /old_locus_tag="pgaptmp_000213"
     CDS             complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHKNP_RS01065"
                     /old_locus_tag="pgaptmp_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGU4702458.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHKNP_RS01070"
                     /old_locus_tag="pgaptmp_000214"
     CDS             complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHKNP_RS01070"
                     /old_locus_tag="pgaptmp_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGU4702459.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHKNP_RS01075"
                     /old_locus_tag="pgaptmp_000215"
     CDS             complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHKNP_RS01075"
                     /old_locus_tag="pgaptmp_000215"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HGU4702460.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHKNP_RS01080"
                     /old_locus_tag="pgaptmp_000216"
     CDS             complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHKNP_RS01080"
                     /old_locus_tag="pgaptmp_000216"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HGU4702461.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHKNP_RS01085"
                     /old_locus_tag="pgaptmp_000217"
     CDS             complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHKNP_RS01085"
                     /old_locus_tag="pgaptmp_000217"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HGU4702462.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHKNP_RS01090"
                     /old_locus_tag="pgaptmp_000218"
     CDS             complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHKNP_RS01090"
                     /old_locus_tag="pgaptmp_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4702463.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHKNP_RS01095"
                     /old_locus_tag="pgaptmp_000219"
     CDS             complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHKNP_RS01095"
                     /old_locus_tag="pgaptmp_000219"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4702464.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6907..7125
                     /gene="slyX"
                     /locus_tag="ACHKNP_RS01100"
                     /old_locus_tag="pgaptmp_000220"
     CDS             6907..7125
                     /gene="slyX"
                     /locus_tag="ACHKNP_RS01100"
                     /old_locus_tag="pgaptmp_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HGU4702465.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHKNP_RS01105"
                     /old_locus_tag="pgaptmp_000221"
     CDS             complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHKNP_RS01105"
                     /old_locus_tag="pgaptmp_000221"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU4702466.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHKNP_RS01110"
                     /old_locus_tag="pgaptmp_000222"
     CDS             complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHKNP_RS01110"
                     /old_locus_tag="pgaptmp_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HGU4702467.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHKNP_RS01115"
                     /old_locus_tag="pgaptmp_000223"
     CDS             complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHKNP_RS01115"
                     /old_locus_tag="pgaptmp_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HGU4702468.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHKNP_RS01120"
                     /old_locus_tag="pgaptmp_000224"
     CDS             complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHKNP_RS01120"
                     /old_locus_tag="pgaptmp_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HGU4702469.1"
                     /translation="MSQPAKVLLLFAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10556..12469
                     /gene="yheS"
                     /locus_tag="ACHKNP_RS01125"
                     /old_locus_tag="pgaptmp_000225"
     CDS             10556..12469
                     /gene="yheS"
                     /locus_tag="ACHKNP_RS01125"
                     /old_locus_tag="pgaptmp_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4702470.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12469..13491
                     /gene="yheT"
                     /locus_tag="ACHKNP_RS01130"
                     /old_locus_tag="pgaptmp_000226"
     CDS             12469..13491
                     /gene="yheT"
                     /locus_tag="ACHKNP_RS01130"
                     /old_locus_tag="pgaptmp_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU4702471.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13485..13703
                     /gene="yheU"
                     /locus_tag="ACHKNP_RS01135"
                     /old_locus_tag="pgaptmp_000227"
     CDS             13485..13703
                     /gene="yheU"
                     /locus_tag="ACHKNP_RS01135"
                     /old_locus_tag="pgaptmp_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HGU4702472.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13757..14626
                     /gene="prkB"
                     /locus_tag="ACHKNP_RS01140"
                     /old_locus_tag="pgaptmp_000228"
     CDS             13757..14626
                     /gene="prkB"
                     /locus_tag="ACHKNP_RS01140"
                     /old_locus_tag="pgaptmp_000228"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HGU4702473.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHKNP_RS01145"
                     /old_locus_tag="pgaptmp_000229"
     CDS             complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHKNP_RS01145"
                     /old_locus_tag="pgaptmp_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HGU4702474.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15387..16019
                     /gene="crp"
                     /locus_tag="ACHKNP_RS01150"
                     /old_locus_tag="pgaptmp_000230"
     CDS             15387..16019
                     /gene="crp"
                     /locus_tag="ACHKNP_RS01150"
                     /old_locus_tag="pgaptmp_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HGU4702475.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHKNP_RS01155"
                     /old_locus_tag="pgaptmp_000231"
     CDS             16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHKNP_RS01155"
                     /old_locus_tag="pgaptmp_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HGU4702476.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHKNP_RS01160"
                     /old_locus_tag="pgaptmp_000232"
                     /gene_synonym="dapC"
     CDS             complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHKNP_RS01160"
                     /old_locus_tag="pgaptmp_000232"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HGU4702477.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHKNP_RS01165"
                     /old_locus_tag="pgaptmp_000233"
     CDS             complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHKNP_RS01165"
                     /old_locus_tag="pgaptmp_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HGU4702478.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHKNP_RS01170"
                     /old_locus_tag="pgaptmp_000234"
     CDS             complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHKNP_RS01170"
                     /old_locus_tag="pgaptmp_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HGU4702479.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHKNP_RS01175"
                     /old_locus_tag="pgaptmp_000235"
     CDS             complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHKNP_RS01175"
                     /old_locus_tag="pgaptmp_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HGU4702480.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHKNP_RS01180"
                     /old_locus_tag="pgaptmp_000236"
                     /gene_synonym="rotA"
     CDS             complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHKNP_RS01180"
                     /old_locus_tag="pgaptmp_000236"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HGU4702481.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHKNP_RS01185"
                     /old_locus_tag="pgaptmp_000237"
     CDS             21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHKNP_RS01185"
                     /old_locus_tag="pgaptmp_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HGU4702482.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23278..25821
                     /gene="nirB"
                     /locus_tag="ACHKNP_RS01190"
                     /old_locus_tag="pgaptmp_000238"
     CDS             23278..25821
                     /gene="nirB"
                     /locus_tag="ACHKNP_RS01190"
                     /old_locus_tag="pgaptmp_000238"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HGU4702483.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25818..26144
                     /gene="nirD"
                     /locus_tag="ACHKNP_RS01195"
                     /old_locus_tag="pgaptmp_000239"
     CDS             25818..26144
                     /gene="nirD"
                     /locus_tag="ACHKNP_RS01195"
                     /old_locus_tag="pgaptmp_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HGU4702484.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26270..27076
                     /gene="nirC"
                     /locus_tag="ACHKNP_RS01200"
                     /old_locus_tag="pgaptmp_000240"
     CDS             26270..27076
                     /gene="nirC"
                     /locus_tag="ACHKNP_RS01200"
                     /old_locus_tag="pgaptmp_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HGU4702485.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLSNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27095..28468
                     /gene="cysG"
                     /locus_tag="ACHKNP_RS01205"
                     /old_locus_tag="pgaptmp_000241"
     CDS             27095..28468
                     /gene="cysG"
                     /locus_tag="ACHKNP_RS01205"
                     /old_locus_tag="pgaptmp_000241"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HGU4702486.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28715..28882
                     /gene="yhfL"
                     /locus_tag="ACHKNP_RS01210"
                     /old_locus_tag="pgaptmp_000242"
     CDS             28715..28882
                     /gene="yhfL"
                     /locus_tag="ACHKNP_RS01210"
                     /old_locus_tag="pgaptmp_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HGU4702487.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            29177..30514
                     /gene="frlA"
                     /locus_tag="ACHKNP_RS01215"
                     /old_locus_tag="pgaptmp_000243"
     CDS             29177..30514
                     /gene="frlA"
                     /locus_tag="ACHKNP_RS01215"
                     /old_locus_tag="pgaptmp_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HGU4702488.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30535..31557
                     /gene="frlB"
                     /locus_tag="ACHKNP_RS01220"
                     /old_locus_tag="pgaptmp_000244"
     CDS             30535..31557
                     /gene="frlB"
                     /locus_tag="ACHKNP_RS01220"
                     /old_locus_tag="pgaptmp_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HGU4702489.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGQLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31607..32437
                     /gene="frlC"
                     /locus_tag="ACHKNP_RS01225"
                     /old_locus_tag="pgaptmp_000245"
     CDS             31607..32437
                     /gene="frlC"
                     /locus_tag="ACHKNP_RS01225"
                     /old_locus_tag="pgaptmp_000245"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HGU4702490.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32434..33219
                     /gene="frlD"
                     /locus_tag="ACHKNP_RS01230"
                     /old_locus_tag="pgaptmp_000246"
     CDS             32434..33219
                     /gene="frlD"
                     /locus_tag="ACHKNP_RS01230"
                     /old_locus_tag="pgaptmp_000246"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HGU4702491.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33319..34050
                     /gene="frlR"
                     /locus_tag="ACHKNP_RS01235"
                     /old_locus_tag="pgaptmp_000247"
                     /gene_synonym="yhfR"
     CDS             33319..34050
                     /gene="frlR"
                     /locus_tag="ACHKNP_RS01235"
                     /old_locus_tag="pgaptmp_000247"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HGU4702492.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(34202..35287)
                     /gene="yhfS"
                     /locus_tag="ACHKNP_RS01240"
                     /old_locus_tag="pgaptmp_000248"
     CDS             complement(34202..35287)
                     /gene="yhfS"
                     /locus_tag="ACHKNP_RS01240"
                     /old_locus_tag="pgaptmp_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGU4702493.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35299..36603)
                     /gene="yhfT"
                     /locus_tag="ACHKNP_RS01245"
                     /old_locus_tag="pgaptmp_000249"
     CDS             complement(35299..36603)
                     /gene="yhfT"
                     /locus_tag="ACHKNP_RS01245"
                     /old_locus_tag="pgaptmp_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HGU4702494.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36615..36968)
                     /gene="yhfU"
                     /locus_tag="ACHKNP_RS01250"
                     /old_locus_tag="pgaptmp_000250"
     CDS             complement(36615..36968)
                     /gene="yhfU"
                     /locus_tag="ACHKNP_RS01250"
                     /old_locus_tag="pgaptmp_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HGU4702495.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36979..37857)
                     /gene="php"
                     /locus_tag="ACHKNP_RS01255"
                     /old_locus_tag="pgaptmp_000251"
     CDS             complement(36979..37857)
                     /gene="php"
                     /locus_tag="ACHKNP_RS01255"
                     /old_locus_tag="pgaptmp_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HGU4702496.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37854..39080)
                     /gene="yhfW"
                     /locus_tag="ACHKNP_RS01260"
                     /old_locus_tag="pgaptmp_000252"
     CDS             complement(37854..39080)
                     /gene="yhfW"
                     /locus_tag="ACHKNP_RS01260"
                     /old_locus_tag="pgaptmp_000252"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGU4702497.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(39080..40243)
                     /gene="yhfX"
                     /locus_tag="ACHKNP_RS01265"
                     /old_locus_tag="pgaptmp_000253"
     CDS             complement(39080..40243)
                     /gene="yhfX"
                     /locus_tag="ACHKNP_RS01265"
                     /old_locus_tag="pgaptmp_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HGU4702498.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPMLAEYGVTHAEPGHALTGTIPANQQGDQPEYIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(40327..40689)
                     /gene="yhfY"
                     /locus_tag="ACHKNP_RS01270"
                     /old_locus_tag="pgaptmp_000254"
     CDS             complement(40327..40689)
                     /gene="yhfY"
                     /locus_tag="ACHKNP_RS01270"
                     /old_locus_tag="pgaptmp_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGU4702499.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40706..41611)
                     /gene="yhfZ"
                     /locus_tag="ACHKNP_RS01275"
                     /old_locus_tag="pgaptmp_000255"
     CDS             complement(40706..41611)
                     /gene="yhfZ"
                     /locus_tag="ACHKNP_RS01275"
                     /old_locus_tag="pgaptmp_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HGU4702500.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41880..42884)
                     /gene="trpS"
                     /locus_tag="ACHKNP_RS01280"
                     /old_locus_tag="pgaptmp_000256"
     CDS             complement(41880..42884)
                     /gene="trpS"
                     /locus_tag="ACHKNP_RS01280"
                     /old_locus_tag="pgaptmp_000256"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGU4702501.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42877..43635)
                     /gene="gph"
                     /locus_tag="ACHKNP_RS01285"
                     /old_locus_tag="pgaptmp_000257"
     CDS             complement(42877..43635)
                     /gene="gph"
                     /locus_tag="ACHKNP_RS01285"
                     /old_locus_tag="pgaptmp_000257"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HGU4702502.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43628..44305)
                     /gene="rpe"
                     /locus_tag="ACHKNP_RS01290"
                     /old_locus_tag="pgaptmp_000258"
     CDS             complement(43628..44305)
                     /gene="rpe"
                     /locus_tag="ACHKNP_RS01290"
                     /old_locus_tag="pgaptmp_000258"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGU4702503.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKLSHE"
     gene            complement(44323..45159)
                     /gene="dam"
                     /locus_tag="ACHKNP_RS01295"
                     /old_locus_tag="pgaptmp_000259"
     CDS             complement(44323..45159)
                     /gene="dam"
                     /locus_tag="ACHKNP_RS01295"
                     /old_locus_tag="pgaptmp_000259"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU4702504.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(45266..46552)
                     /gene="damX"
                     /locus_tag="ACHKNP_RS01300"
                     /old_locus_tag="pgaptmp_000260"
     CDS             complement(45266..46552)
                     /gene="damX"
                     /locus_tag="ACHKNP_RS01300"
                     /old_locus_tag="pgaptmp_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HGU4702505.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46644..47732)
                     /gene="aroB"
                     /locus_tag="ACHKNP_RS01305"
                     /old_locus_tag="pgaptmp_000261"
     CDS             complement(46644..47732)
                     /gene="aroB"
                     /locus_tag="ACHKNP_RS01305"
                     /old_locus_tag="pgaptmp_000261"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGU4702506.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADSQSA"
     gene            complement(47789..48310)
                     /gene="aroK"
                     /locus_tag="ACHKNP_RS01310"
                     /old_locus_tag="pgaptmp_000262"
     CDS             complement(47789..48310)
                     /gene="aroK"
                     /locus_tag="ACHKNP_RS01310"
                     /old_locus_tag="pgaptmp_000262"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HGU4702507.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48711..49949)
                     /gene="hofQ"
                     /locus_tag="ACHKNP_RS01315"
                     /old_locus_tag="pgaptmp_000263"
     CDS             complement(48711..49949)
                     /gene="hofQ"
                     /locus_tag="ACHKNP_RS01315"
                     /old_locus_tag="pgaptmp_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HGU4702508.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49861..50265)
                     /gene="hofP"
                     /locus_tag="ACHKNP_RS01320"
                     /old_locus_tag="pgaptmp_000264"
     CDS             complement(49861..50265)
                     /gene="hofP"
                     /locus_tag="ACHKNP_RS01320"
                     /old_locus_tag="pgaptmp_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HGU4702509.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(50255..50695)
                     /gene="hofO"
                     /locus_tag="ACHKNP_RS01325"
                     /old_locus_tag="pgaptmp_000265"
     CDS             complement(50255..50695)
                     /gene="hofO"
                     /locus_tag="ACHKNP_RS01325"
                     /old_locus_tag="pgaptmp_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HGU4702510.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50679..51218)
                     /gene="hofN"
                     /locus_tag="ACHKNP_RS01330"
                     /old_locus_tag="pgaptmp_000266"
     CDS             complement(50679..51218)
                     /gene="hofN"
                     /locus_tag="ACHKNP_RS01330"
                     /old_locus_tag="pgaptmp_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HGU4702511.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(51218..51997)
                     /gene="hofM"
                     /locus_tag="ACHKNP_RS01335"
                     /old_locus_tag="pgaptmp_000267"
     CDS             complement(51218..51997)
                     /gene="hofM"
                     /locus_tag="ACHKNP_RS01335"
                     /old_locus_tag="pgaptmp_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HGU4702512.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            52117..54669
                     /gene="mrcA"
                     /locus_tag="ACHKNP_RS01340"
                     /old_locus_tag="pgaptmp_000268"
     CDS             52117..54669
                     /gene="mrcA"
                     /locus_tag="ACHKNP_RS01340"
                     /old_locus_tag="pgaptmp_000268"
                     /EC_number="2.4.99.28"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HGU4702513.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54835..55395)
                     /gene="nudE"
                     /locus_tag="ACHKNP_RS01345"
                     /old_locus_tag="pgaptmp_000269"
     CDS             complement(54835..55395)
                     /gene="nudE"
                     /locus_tag="ACHKNP_RS01345"
                     /old_locus_tag="pgaptmp_000269"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HGU4702514.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55715..57850
                     /gene="igaA"
                     /locus_tag="ACHKNP_RS01350"
                     /old_locus_tag="pgaptmp_000270"
     CDS             55715..57850
                     /gene="igaA"
                     /locus_tag="ACHKNP_RS01350"
                     /old_locus_tag="pgaptmp_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HGU4702515.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWVPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57915..58583
                     /gene="yrfG"
                     /locus_tag="ACHKNP_RS01355"
                     /old_locus_tag="pgaptmp_000271"
     CDS             57915..58583
                     /gene="yrfG"
                     /locus_tag="ACHKNP_RS01355"
                     /old_locus_tag="pgaptmp_000271"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HGU4702516.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58594..58995
                     /gene="hslR"
                     /locus_tag="ACHKNP_RS01360"
                     /old_locus_tag="pgaptmp_000272"
     CDS             58594..58995
                     /gene="hslR"
                     /locus_tag="ACHKNP_RS01360"
                     /old_locus_tag="pgaptmp_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HGU4702517.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            59020..59898
                     /gene="hslO"
                     /locus_tag="ACHKNP_RS01365"
                     /old_locus_tag="pgaptmp_000273"
     CDS             59020..59898
                     /gene="hslO"
                     /locus_tag="ACHKNP_RS01365"
                     /old_locus_tag="pgaptmp_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGU4702518.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILTEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(60033..61757)
                     /gene="yhgE"
                     /locus_tag="ACHKNP_RS01370"
                     /old_locus_tag="pgaptmp_000274"
     CDS             complement(60033..61757)
                     /gene="yhgE"
                     /locus_tag="ACHKNP_RS01370"
                     /old_locus_tag="pgaptmp_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HGU4702519.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYKSVWHNVLILLVIFLANGLTWVVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            62136..63758
                     /gene="pckA"
                     /locus_tag="ACHKNP_RS01375"
                     /old_locus_tag="pgaptmp_000275"
     CDS             62136..63758
                     /gene="pckA"
                     /locus_tag="ACHKNP_RS01375"
                     /old_locus_tag="pgaptmp_000275"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HGU4702520.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKELGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63834..65186)
                     /gene="envZ"
                     /locus_tag="ACHKNP_RS01380"
                     /old_locus_tag="pgaptmp_000276"
     CDS             complement(63834..65186)
                     /gene="envZ"
                     /locus_tag="ACHKNP_RS01380"
                     /old_locus_tag="pgaptmp_000276"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HGU4702521.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIDVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(65183..65902)
                     /gene="ompR"
                     /locus_tag="ACHKNP_RS01385"
                     /old_locus_tag="pgaptmp_000277"
     CDS             complement(65183..65902)
                     /gene="ompR"
                     /locus_tag="ACHKNP_RS01385"
                     /old_locus_tag="pgaptmp_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HGU4702522.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            66130..66606
                     /gene="greB"
                     /locus_tag="ACHKNP_RS01390"
                     /old_locus_tag="pgaptmp_000278"
     CDS             66130..66606
                     /gene="greB"
                     /locus_tag="ACHKNP_RS01390"
                     /old_locus_tag="pgaptmp_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HGU4702523.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66703..69024
                     /gene="yhgF"
                     /locus_tag="ACHKNP_RS01395"
                     /old_locus_tag="pgaptmp_000279"
     CDS             66703..69024
                     /gene="yhgF"
                     /locus_tag="ACHKNP_RS01395"
                     /old_locus_tag="pgaptmp_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGU4702524.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVMSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQTAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69462..69689
                     /gene="feoA"
                     /locus_tag="ACHKNP_RS01400"
                     /old_locus_tag="pgaptmp_000280"
     CDS             69462..69689
                     /gene="feoA"
                     /locus_tag="ACHKNP_RS01400"
                     /old_locus_tag="pgaptmp_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HGU4702525.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69706..72027
                     /gene="feoB"
                     /locus_tag="ACHKNP_RS01405"
                     /old_locus_tag="pgaptmp_000281"
     CDS             69706..72027
                     /gene="feoB"
                     /locus_tag="ACHKNP_RS01405"
                     /old_locus_tag="pgaptmp_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HGU4702526.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            72027..72263
                     /gene="feoC"
                     /locus_tag="ACHKNP_RS01410"
                     /old_locus_tag="pgaptmp_000282"
     CDS             72027..72263
                     /gene="feoC"
                     /locus_tag="ACHKNP_RS01410"
                     /old_locus_tag="pgaptmp_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HGU4702527.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72466..73344
                     /gene="rpnA"
                     /locus_tag="ACHKNP_RS01415"
                     /old_locus_tag="pgaptmp_000283"
     CDS             72466..73344
                     /gene="rpnA"
                     /locus_tag="ACHKNP_RS01415"
                     /old_locus_tag="pgaptmp_000283"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HGU4702528.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(73373..74143)
                     /gene="bioH"
                     /locus_tag="ACHKNP_RS01420"
                     /old_locus_tag="pgaptmp_000284"
     CDS             complement(73373..74143)
                     /gene="bioH"
                     /locus_tag="ACHKNP_RS01420"
                     /old_locus_tag="pgaptmp_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HGU4702529.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            74181..74864
                     /gene="gntX"
                     /locus_tag="ACHKNP_RS01425"
                     /old_locus_tag="pgaptmp_000285"
     CDS             74181..74864
                     /gene="gntX"
                     /locus_tag="ACHKNP_RS01425"
                     /old_locus_tag="pgaptmp_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HGU4702530.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            74923..75498
                     /gene="nfuA"
                     /locus_tag="ACHKNP_RS01430"
                     /old_locus_tag="pgaptmp_000286"
     CDS             74923..75498
                     /gene="nfuA"
                     /locus_tag="ACHKNP_RS01430"
                     /old_locus_tag="pgaptmp_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HGU4702531.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            75859..77175
                     /gene="gntT"
                     /locus_tag="ACHKNP_RS01435"
                     /old_locus_tag="pgaptmp_000287"
     CDS             75859..77175
                     /gene="gntT"
                     /locus_tag="ACHKNP_RS01435"
                     /old_locus_tag="pgaptmp_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU4702532.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDCGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77220..79304)
                     /gene="malQ"
                     /locus_tag="ACHKNP_RS01440"
                     /old_locus_tag="pgaptmp_000288"
     CDS             complement(77220..79304)
                     /gene="malQ"
                     /locus_tag="ACHKNP_RS01440"
                     /old_locus_tag="pgaptmp_000288"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HGU4702533.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79314..81707)
                     /gene="malP"
                     /locus_tag="ACHKNP_RS01445"
                     /old_locus_tag="pgaptmp_000289"
     CDS             complement(79314..81707)
                     /gene="malP"
                     /locus_tag="ACHKNP_RS01445"
                     /old_locus_tag="pgaptmp_000289"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HGU4702534.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKMIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            82319..85024
                     /gene="malT"
                     /locus_tag="ACHKNP_RS01450"
                     /old_locus_tag="pgaptmp_000290"
     CDS             82319..85024
                     /gene="malT"
                     /locus_tag="ACHKNP_RS01450"
                     /old_locus_tag="pgaptmp_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HGU4702535.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(85067..86083)
                     /gene="rtcA"
                     /locus_tag="ACHKNP_RS01455"
                     /old_locus_tag="pgaptmp_000291"
     CDS             complement(85067..86083)
                     /gene="rtcA"
                     /locus_tag="ACHKNP_RS01455"
                     /old_locus_tag="pgaptmp_000291"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HGU4702536.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(86087..87313)
                     /gene="rtcB"
                     /locus_tag="ACHKNP_RS01460"
                     /old_locus_tag="pgaptmp_000292"
     CDS             complement(86087..87313)
                     /gene="rtcB"
                     /locus_tag="ACHKNP_RS01460"
                     /old_locus_tag="pgaptmp_000292"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HGU4702537.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        20826 a        23886 c        22067 g        20578 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcatat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag
      481 gttttagcca gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg
      541 ccgatagtac caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct
      601 atattcctta ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc
      661 ttaggcttat ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc
      721 atacttcagg aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt
      781 tgcgtatccg aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc
      841 agattcctga cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt
      901 gttctcttcc ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc
      961 tttcttaaag ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga
     1021 gtcaacgtca tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc
     1081 tgccagcgga cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc
     1141 gccagggatt acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc
     1201 cggctccagc gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg
     1261 tttcgcgtgt ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac
     1321 ctgcggttta cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat
     1381 gtcgaggtgc agttcgccca tacccgcgat aatggtctgg ttagattctt cgtcagtcca
     1441 tacacggaaa gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg
     1501 gtcagctttg gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg
     1561 ttccagaatg atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag
     1621 accgatagca gcagcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc
     1681 gtgcatctga acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt
     1741 atcaccagag ttaaccacac cggagtaaac acggaagaag gtcaggttac caacaaacgg
     1801 gtcggtagcg attttgaacg ccagtgcaga aaacggctcg tcatcacttg cgtgacgctc
     1861 agccggagtg tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga
     1921 tggcaggtaa tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga
     1981 accacaggtt accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat
     2041 ttctgcttca gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc
     2101 agctgcggat tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg
     2161 gatatcttcg tattcgaagg ttacgccctg gtcagcgtcg ttccagttga tagctttcat
     2221 tttcaccagg tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg
     2281 aaccgggttc gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc
     2341 catgcggtcc attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg
     2401 ccatacggtt tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc
     2461 atcgagaaca cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc
     2521 gatgatgttg atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt
     2581 cgcagcggaa gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc
     2641 agcgccgtca tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc
     2701 ggtagtagtg gttttaccgg cgtcgatgtg cgcactgata ccgatattac ggtagcgtgc
     2761 gatgggtgtt gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta
     2821 gcccaaagcg ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta
     2881 ccaacggtag tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt
     2941 cttaactgca gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc
     3001 catggattta tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt
     3061 acgacgaacc ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga
     3121 cttaacttct acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga
     3181 tttaccagag cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga
     3241 ttttttacca tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt
     3301 cggatccggc agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg
     3361 ttgttaattc aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt
     3421 ttggccttac ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg
     3481 agcctgctta cggtctttaa cgccggagca gtcaagcgca ccacgtacgg tgtggtaacg
     3541 aacacccggg aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag
     3601 gttgtgacct tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg
     3661 gcatacttta cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca
     3721 tacgccacgt ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg
     3781 agcacgtggt ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc
     3841 ttttgcttcg taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag
     3901 ggcgatgccg aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg
     3961 ctggagtgct taaccgtaag tctgacgaaa tcagtatagt caatgggaat gatgtcgttc
     4021 gaaatttgac cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg
     4081 ggggcattac gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacgccatct
     4141 tgcaataata gcagttcgtc tccttcactg agcagacgca gcagcgcagc aaaatccgtc
     4201 agccagggtg aacgatgtaa cgtgtgcagc ataaacgcct caaaacctca aaataacatc
     4261 gtagttggcg agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa
     4321 gggtgtctgc ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc
     4381 aatgtcgtac agacccaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactgcatc
     4441 gggcttttgt cctgacagca gctgaaaaac gccatcagca ataaagaaga cggccagatc
     4501 gtcagttaat gcggaagttg ccagtaaagc atctaaacct tcccgaccag cagctgtacc
     4561 atgaggtgca gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca
     4621 caggtcagcg aggcttccgc cagcgcgcca agtccgctta aggtaaatcc cggctgaagg
     4681 tttgacgaag ccagccccag ccttcctgct tcagtttcat cgacgatacc gcggcgtaac
     4741 gctgccgcta cgcagatatt cagcgccaca ccatgttgcg cattcaactg ttgccagccg
     4801 cgcacaaggt caaattcgtc acttgccggt gaggtcaatt gattagcgtt atagacccct
     4861 tcccgataga aaaagacgct gcttaactca tggccttcta ctatcagcgc ctgcgcaaac
     4921 tgaaaagcac tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa
     4981 cgcattactt atcttgcccc tggaaatcac cgcttttgaa ctggcggatg tagagataga
     5041 cagtgtgttt ggagatgttc aggcggtcag caacctggtt gatcgcgtct ttaatatcga
     5101 agatcccttt ctcgtagaga ttcagcacga tctgacgatt tttggcgtta ttagaaacat
     5161 tgcgatcggc attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcaa
     5221 cagaagaggc aaagttgacg cttgaaccga catccggggt ttctggcggc acaaaagtgc
     5281 tcataatctg cgagaaggga acatcaagat tcatattgat gcacagcaga ccaattacac
     5341 gctgttcgcg gttacgaatc gcgatagtca gggacttcat taatacgccg cttttggcgc
     5401 gagtaaagta gcatttagaa acgctgctat ccgctcccgt catatcgtgc agcatacgta
     5461 gcgccaggtc agtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatgc
     5521 gaatggctga acattttaga tcctgcaaag agtgcaaaac gatttcacag tgggagccaa
     5581 taagcatcgc taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcggtct
     5641 ggtcgaaagg acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc
     5701 tggacatgaa aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc
     5761 attaatgatg cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat
     5821 atccggcctg taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt
     5881 ttagcagaat ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt
     5941 ttcacatcca gcagctctac gtcaaacacc agggtagaat tcggtgggat ccccggaaca
     6001 cccgctttgc cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg
     6061 atgttcttca gaccttctgt ccaacccggg ataacaccgt ccagacggaa agaaagcggt
     6121 tcaccacggg tgtaagagtt gtcgaactct ttaccgtcga tcagcgtacc tttgtagttc
     6181 actacaacag tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa
     6241 accagaccag ttgaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct
     6301 ttaccttttg cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc
     6361 acgcgagctt cgaatgcttg cagagtctgt tcgatctctt ggtcggagag tttgctctta
     6421 tcagcaaatg catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt
     6481 ttttcttgtt ctttaagaga gttttccatg taacgaccca gcgaagcacc cagtgcataa
     6541 gctgatttct gatcgtcatt tttgaacgct gctttgctgt cagcagttgt agcaggtttt
     6601 gcagcttcag cagcaaaagt gattggtgca tgcagggcaa cggccattgt ggtcgccagc
     6661 agcgttactt taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg
     6721 gttaactatt atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg
     6781 ccctattatc atctattttc agactctttt tgtttaaatt agtttcgatg accgcgaaat
     6841 gagtgctgtc tcgggcagca aagttaagta gaatccgcgg cgaccattcg acaaaagagg
     6901 tgaatcatgc aggatttatc attggaagca cgcctggcag agctggagag ccgactggct
     6961 tttcaggaaa tcaccattga agaactgaac gtcacggtga ccgctcatga aatggagatg
     7021 gcgaaactgc gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg
     7081 aacatcgcgt cgcaggctga agaaacgcca ccgccacatt attgaggcgt aaaaaaagcg
     7141 gggattcccc gcttttttgt caccttttcg gtattagtgg caaccacaac cgccgttgcc
     7201 tttaccgcca cagcagcctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc
     7261 gcagcaaccg tcgtggtcgt gatcgtggtg atgatcgtgc gcgccgtgaa cgtggccatg
     7321 agccagttct tcttcggtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt
     7381 ctgaccggcc agcatgtggt taccatcaac cacgacgtga tcgtcttcaa ccgcagtgat
     7441 ttcaaccggt accggcccct ggtcggtttc agccaggaaa cgcataccta cctgcagttc
     7501 atcaacgccc ataaatacgt ctttaggaac acgttgcacc aggttttcgt cgtactgacc
     7561 gtaagcgtcg ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag
     7621 cgccgtttcc aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact
     7681 caccggagac tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac
     7741 caccaggtct tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga
     7801 ttgtagcgga attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct
     7861 agtccggatg aaaaatcccg atcacttgct catctttgcg aacgtgatcg cgggcttctt
     7921 tgtctgcttc tcgcatctga tgtccgcact taacacattc aacaatatca atattatttt
     7981 cgcgccacat cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgcgataa
     8041 aacgttttcg gattgccatc tttaccctct actcaaattc atcccagccg tccagctggc
     8101 gtcgttcttg ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg
     8161 cgcgagaaat ttgtacggta tcagcatgca tcgggattaa ctcccgcagc attcgcatat
     8221 ccaggcggcg aaaatgcagt tgcgcccgct gtgcctgatg cggatgcatg ccaagcgtga
     8281 ccagcgtctt gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca
     8341 cccctgcctg taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca
     8401 aatgcggaaa gtgctgctgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac
     8461 aggtaatgac gatagactca gcggcctctg cacccgcaga acgtaaaaga tcgacctgcg
     8521 tggcgtcgcc gtaataaact ttgtagccat atttacgcat caggttaacg gcgctgatat
     8581 cccgctccag cacggtaatg cgcattttat ttgccatcag caaacgacca atcacctgac
     8641 caaaacgccc gaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt
     8701 cgtcttcttc ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca
     8761 gcaacggcgt ggtcatcatg gaaagcgtca ccgtcaccag caacagcgcc atctggtcgc
     8821 cctggaataa acgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccgccct
     8881 gactcaacac gccagcaaac tgcatccgct ctgagctacg cacgccatac aatcgcgcca
     8941 gcagatacag cacgagaatt ttcaccgcca ccagcacaac cacgcttatc actacccaca
     9001 acagatgggt ataaagcacc ccgaggttga gcgacatgcc gacagagata aagaacaaac
     9061 cgagcagcaa tcctttgaag ggatcgatag ccgtttccag ttcatggcga tattcacttt
     9121 ccgccagcag cacgcccgca ataaatgtac cgagcgccat cgacagcccc agcgcatcca
     9181 taaacaatgc ggaacccaac accagcagca gcgtcgcggc ggtgaacact tcccgcacgc
     9241 cagaagctgc aataaagcgg aataccggac gcagtaaata gcgcccacca atcagcatgc
     9301 cgacaaacgc cagcaccttc atgccgatct tcatccagtc gaaatgttcg tctgccgaac
     9361 ccgccaacaa cggcactaac gccagtgccg ggatcaccgc cagatcctga aacagcagta
     9421 ccgaaaaccc gagctgcccg gattcgctgc gattcatccc tttctcacgc atcaattgca
     9481 acgccattgc ggtcgaagac atcgcaaggc caatgccacc gaccaccgcc gcctgccagg
     9541 cgaaatccgt cagcatcaat aatcccgcca gcaacgccgc gcttaacagc acctgtgccg
     9601 cgcctacgcc aaaaatcgaa cgccgcagtt gccaaagctt ggagggattc aactcaaggc
     9661 cgatgataaa catcaggaat accacgccga gttccgaaaa gtggaggatc tcatcgacgt
     9721 cgctaataaa ccccagcccc cacgggccaa ttgcaatccc tgccagcaaa tatcccaaca
     9781 cagcgccaat ccccagccgc gatgccaacg gcaccgcagc caccgccgcg aagagaaaca
     9841 gcactcctgc gagtaaaaaa tcggaacctt ccatcagcgg cctcctggag acagcggatt
     9901 tgccagccag tcaccgtagg ctctggcgtg gctcgccagc tcctgtgcgc tttgccgtct
     9961 cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg ccgccagttc
    10021 aaagggacgc agaacgtcgc tcatggggta gcgattcaat gcgtcataac ggtaagcact
    10081 ttccggctcg ccggtggtga tcacgctacg ccagtacttt cccgccagtt ggtttcctcc
    10141 cggcccgctg gcaaaaccac ggcttaatac ccggtccagc cactctttca gtagcgccgg
    10201 gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc
    10261 ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcataaaggt cgtgcacggt
    10321 aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg agtcctgaga
    10381 ttccggatgg gcaaacagca gcaaaacttt cgctggctga gacatcatcc ccctcccggt
    10441 atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc
    10501 catacattac attatcataa tgataagtta acatagtctg aacatacggc accttatgat
    10561 tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc
    10621 caccatcaac cctgggcaga aagtcggcct ggtgggtaaa aacggctgtg gtaaatctac
    10681 cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca cctttccggg
    10741 aagctggcaa ctggcgtggg tgaatcagga aacgccggcg ttaccgcaag cggcgctgga
    10801 atatgtcatt gacggcgacc gtgaatatcg tcaactagaa gcgcagctac acgacgccaa
    10861 cgaacgtaac gacgggcacg ccattgcgac cattcatggc aagctggatg ctattgacgc
    10921 atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca gcaatgaaca
    10981 actggagcgc ccggtaagtg atttctccgg tggctggcgt atgcgtctta accttgccca
    11041 ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc acctcgatct
    11101 cgatgccgtt atctggctgg aaaaatggct gaagagctat cagggcacgc tgatcctgat
    11161 ctctcacgac cgcgacttcc tcgatccgat cgtcgataaa attattcata tcgaacaaca
    11221 aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct
    11281 ggcgcagcaa caagcgatgt acgaaagcca gcaggaacgc gtagcgcatc tgcaaagtta
    11341 tatcgaccgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc gcattaagat
    11401 gctcgagcgt atggagctaa ttgcccccgc gcacgtcgac aacccgttcc gctttagctt
    11461 ccgcgcgccg gaaagcctgc caaatccgtt actgaagatg gaaaaagtca gcgcgggcta
    11521 tggcgatcgc attattctcg actcgattaa actgaacctg gtgcccggct cgcgcattgg
    11581 tctgttaggc cgcaatggcg cgggtaaatc gacattaatc aaactgttag ccggtgaact
    11641 tgcgccagtc agcggtgaaa ttggtctggc gaaagggatc aaactcggct acttcgccca
    11701 gcatcaactt gaatacctgc gcgccgacga atcacctatt caacatctgg cacgtttagc
    11761 gccgcaggag ctggaacaaa aactgcgtga ctacctcggc ggctttggtt tccagggcga
    11821 taaagtaacc gaagaaacgc gccgcttctc cggtggggaa aaagcccgcc tggtgctggc
    11881 attaattgtc tggcagcggc cgaatctgct gctgctcgac gaaccgacta accaccttga
    11941 cctcgacatg cgtcaggcac tcaccgaagc attaatcgag tttgaaggcg cgctggttgt
    12001 cgtttcgcac gaccgtcatt tgctgcgttc caccactgac gatctctacc tggttcacga
    12061 tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt tgagcgacgt
    12121 acaaaagcag gaaaaccaga ccgacgaagc gccaaaagag aatgcgaaca gcgcccaggc
    12181 acgtaaagat cagaagcgtc gggaagctga gctgcgtgcg caaacccagc cactgcgtaa
    12241 agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg cgcaggcgga
    12301 agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt tgaccgcctg
    12361 cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg catggctgga
    12421 agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat ggcgcagata
    12481 acgacgaccg atgccaatga attcagcagc agtgctgaat tcatccccat gcgcggcttt
    12541 agcaattgtc atctgcaaac catgctgccg cgtctgtttc gtcgtcaggt gaaattcacc
    12601 ccgtactggc agcggctgga gttgcccgac ggcgattttg tcgatcttgc gtggagtgaa
    12661 aaccctgcac aggcgcaaca taaacctcgt ctggtggtgt ttcacgggct ggaaggcagt
    12721 ctcaacagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggc
    12781 gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg catttaccat
    12841 tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga atttggtcat
    12901 gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg tttgctggca
    12961 aaggaaggca atgatctccc ggttgatgcg gcggtgattg tctctgcgcc gtttatgctg
    13021 gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg ttacttgctg
    13081 aacctgttaa aagccaatgc cgcgcgcaag ctggcagcct accccggaac gctgccgatt
    13141 aatctcgcgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct catcaccgcc
    13201 agaattcacg gctacgccga cgctatcgac tattatcgtc agtgtagcgc catgccgatg
    13261 ctgaaccgga tcgccaaacc gacgctgatt attcacgcca aagacgatcc gtttatggat
    13321 catcaggtga tcccgaaacc ggaaagtctc cccccgcagg tggagtatca actgactgaa
    13381 catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat gtggctggag
    13441 tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg attccgtggc
    13501 aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca
    13561 ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc aaacgccagc
    13621 tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc aatattatgc
    13681 cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat aatgatagct
    13741 aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag gatccagcgg
    13801 cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt taaatctgca
    13861 tgcagctgag gtggaaggcg acagttttca ccgttacacc cgcccggaaa tggacatggc
    13921 gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg aggctaacga
    13981 cttcggcctg ctggaacaaa ccttcattga atacggtcag agcggcaaag ggaaatctcg
    14041 caaatatctg catacctacg acgaagccgt accgtggaat caggtaccgg ggacattcac
    14101 cccctggcaa cctttaccgg aacccactga tgtactgttt tatgaaggtt tacacggcgg
    14161 cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg gcgtggtgcc
    14221 tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc gcgggcactc
    14281 acgagaagca gtgatggact cagtagtgcg ttcaatggaa gactatatca actacatcac
    14341 accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg acacttcaaa
    14401 cccgttcgcg gcaaaaggta tcccgtcgct cgatgaaagc tttgtggtga tccattttcg
    14461 taatctggaa gggatcgatt tcccctggct gctggcgatg ttgcaaggct cattcatttc
    14521 ccacatcaat acgttagtgg taccgggcgg caaaatgggt ctggcaatgg aattaattat
    14581 gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaactct gctattacgc
    14641 cggataaaat actatccggc ttcacaacgg gatagttaag tcacgcggca accacttcat
    14701 acgagtgagt aatattcacc gctttttcca gcatcaacgc cactgaacaa tatttctcgg
    14761 cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg gtgacgataa
    14821 aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca gaggtcaatt
    14881 ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatcgaaacc acatcgatgg
    14941 cactgcaacc acccgccgcc atcaacacca tttccatcgg acttggtgct ttatcgcctg
    15001 agttgccgtc cattaaaatc tgatgaccag aggcggattc gcccagaaaa gttaaccctt
    15061 cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt tcctgacaga
    15121 gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg aagcgagaca
    15181 ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt aatacattga
    15241 tgtactgcat gtatgcaaag gacgtcacat taccgtgcag tacagttgat agccccttcc
    15301 caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg gctctggaga
    15361 aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca gacccgactc
    15421 tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc acgcttattc
    15481 accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg gcagtgctga
    15541 tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt gattttattg
    15601 gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt gcgaaaaccg
    15661 cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag gtaaacccgg
    15721 acattctgat gcgtttgtct gcacagatgg cgcgtcgtct gcaagtcact tcagagaaag
    15781 tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg ctgaatctgg
    15841 caaaacaacc agacgctatg actcacccgg acggtatgca aatcaaaatt acccgtcagg
    15901 aaattggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg aagatgctgg
    15961 aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc actcgttaat
    16021 cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga tgtggcgcag
    16081 actgatttat caccccgata tcaactatgc acttcgacaa acgctggtgc tatgtttgcc
    16141 cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct ctctcgttcc
    16201 tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca aacgcttaat
    16261 cattggtgcg tcgctgtttg ccacctgtag cttactgaca cagctgctac tggcaaaaga
    16321 tgttcccctg ccctttttgc tgaccggatt aacgctggta cttggcgtca ctgctgagtt
    16381 ggggccattg cacgcaaaat tgcttcccgc atcgctgctg gcagccattt ttaccctcag
    16441 tctggcggga tacatgccgg tctgggaacc gttgctcatc tatgcgttgg gcactctctg
    16501 gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc tgcgcgagtc
    16561 actaagtctg ctgtaccgtg aactggcaga ttattgtgaa gccaaataca gcctgcttac
    16621 ccagcacacc gaccctgaaa aagcgctgcc gccgctgctg gtgcgccagc aaaaagcggt
    16681 cgatctaatt acccagtgct atcagcaaat gcatatgctt tccgcgcaaa ataatactga
    16741 ctacaagcgg atgctgcgta ttttccagga ggcgctggat ttacaggaac atatttcggt
    16801 cagtttgcat cagccggaag aggtgcaaaa gctggtcgag cgtagccatg cggaagaagt
    16861 tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg atgacattct
    16921 ttaccatcgc ctgccaacgc gttttacgat ggaaaagcaa attggcgcac tggaaaaaat
    16981 cgcccgccag catccggata atccggttgg gcaattctgc tactggcatt tcagccgcat
    17041 cgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcacgt gacttactgg ccgataaaca
    17101 gcggcgaatg ccattacttc cggcgctgaa aagttatctg tcactaaagt ctccggcgct
    17161 acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg gcaccgcgct
    17221 gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacac aaaatggcta
    17281 tggcgcaacc cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg ggttaatcat
    17341 tgcgggcgtg gcgctgcact ttaaaattcc cgaaggttac accctgacgt tgatgctgat
    17401 taccaccctc gccagctacc tgatattgcg caaaaactac ggctgggcga cggtcggttt
    17461 tactattacc gcagtgtata ccctgcaact attgtggttg aacggcgagc aatacatcct
    17521 tccgcgtctt atcgatacca ttattggttg tttaattgct ttcggcggta ctgtctggct
    17581 gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgacgctt tagaagccta
    17641 tcaggaagcg attcgcttga ttcttagcga ggatccgcaa cctacgccac tggcctggca
    17701 gcgaatgcgg gtaaatcagg cacataacac tctgtataac tcattgaatc aggcgatgca
    17761 ggaaccggcg tttaacagcc attatctggc agatatgaaa ctgtgggtaa cgcacagcca
    17821 gtttattgtt gagcatatta atgccatgac cacgctggcg cgggaacacc gggcattgcc
    17881 acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc gttgtcagca
    17941 gcgactggag tatgacgaac cgggtagttc tggcgatgcc aatatcatgg atgcgccgga
    18001 gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc gggttattgg
    18061 tcatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac cgcatcacgg
    18121 gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tgcaggctaa accatcggat
    18181 aaggcattca cgccgcatcc gacatctttt gcctgatgct tcgtatttac gccccaacca
    18241 ccttcgccac cgcgtgggcg aaacgttgca tcccttcatc gatatccgca tcttccacca
    18301 ccagcgacgg tgcaaaacgc atcacatccg gtccggcatt cagcaccatt acgccagcct
    18361 ctgcgcccgc atacaggaaa tcacgcgccc gacctttgta ctgtggtttc agctctgcgc
    18421 caatcaacag ccccataccg cgaatatcgc taaacacatc gtactgctga tcgatcttct
    18481 gcagatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact tcaggggtat
    18541 tgatgatatc aaacgccgcc cccgctactg cacaggccag aggattaccg ccgtaggtgg
    18601 aaccgtgaga accaggatga aacgcagaag caatttctgc cgtggtcagc atggcgctaa
    18661 tcgggaagcc gccgcctaac gctttcgcag aggtcagaat atccggcgtc acgccgtagt
    18721 gcatgtaagc aaacaaatcg ccggtccgcc ccatcccgca ctgtacttca tcaaacacca
    18781 ataatgcctg atgttgatcg cacagctcgc gcaagccctg caaaaactct ggcgtcgctg
    18841 ccgtcacacc gccctcgccc tggatcggct caaccaccac cgcacaggtg tgatcatcca
    18901 tcaccgcttt cactgcgtgg agatcgttaa aaggaacgtg gatgatgtct gccggttttg
    18961 gcccaaagcc gtcggaatat tttggctgcc cgcccaccga gacggtaaac agcgagcgac
    19021 catgaaaagc gttatggaag gcaataattt tggttttgaa cggactatga cgcacacagg
    19081 cgtaatggcg tgccagttta aaggcggttt cgttagcttc cgtgccggag ttcataaaca
    19141 ccacgcgttc ggcaaacgtc gcctcaatca gtttgcgccc aagacgcagc gccggttcat
    19201 tggtgaaaac gttactgata tgccacagag tttcgccctg ggtttttaac gcgttcacca
    19261 gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa tcgacatact
    19321 ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaatg aactctgccg
    19381 gagcataaat cggcaggatc acttcatcga aagtcgcgcg tgtaattgct gtttgttcaa
    19441 ttgccatctc atgatcaccc tgttacgcat aaataaatgt gaaattataa tcacaaaata
    19501 tgcataaaaa atcattgaat ggcaatcaga aatcagcgat gcaggaaatt agccagcagt
    19561 tgatgtcctt gttcgctaag aatactttct ggatggaact gcacaccttc cagatcccac
    19621 tggcgatggc gaatccccat aatctcgcgg gtttcgctcc aggccgtcac gtcaaagcat
    19681 gctggtaatg agccaggttc caccaccagc gaatggtagc gtgtcacggt aagtggattt
    19741 gccagccccc gaaatacgcc ctcaccgtta tgtgtaatcg gcgaggtttt gccgtgcatg
    19801 acctttgcgg cgcgcacaac tttgccgcca aatgcctgcg ccattgcctg atgaccgagg
    19861 cagacgccaa gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag ggagatcccg
    19921 gcttcatctg gcgtacaggg gccgggtgat atgacaattt tttgtggttt aagggcgtcg
    19981 atatccgcca gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc cagttcacaa
    20041 aagtactggt agaggttcca ggtaaaagaa tcgtagttat ctataagcag gatcatggcg
    20101 gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggcttcgc ttaccacttt
    20161 gctaaatatc atctgcagcg cggtgagatc ccccattgcc ccactctgat ttgcctgatt
    20221 ccaggcctct ttttcgatac cctgccagct cagttgatac ccggcatgaa tcgccagttg
    20281 ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca gcacgttgat
    20341 ttcacaatag taatgcgcca gccgctcgac gaacttcgct ttctccaggc caaccagata
    20401 accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat aagcaaagtg
    20461 gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccccg cccagtcgaa
    20521 aatatcctga tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc gcaccagcgg
    20581 accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct gttccagccg
    20641 ctgctgctgg tggatgttca gccggttacg catgatatca aggccaggat aaagatacgg
    20701 atcgcgccct tcgccgaatt tatcgctcat agtgcctcct cagctcttca aggcgcgcta
    20761 aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga cggcgactgg
    20821 cctggaaatt acgattacgc tgaagctccc agagacggga cttttgctta tcggtgagtt
    20881 tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg gcaaatccgg
    20941 gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgaaaaatc attacggcag
    21001 gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacgtc
    21061 atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca ctttaccaaa
    21121 taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg caacgttgat
    21181 aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga tggtgccacg
    21241 cgtgttgcgc aggccgttat cggcttcatt tttgattggc gggtttggtt ttttctgctg
    21301 catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt
    21361 agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag acactggcgc
    21421 tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg
    21481 gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcgaaaa cagcagccat
    21541 cgccgccagg gtcgatttga acatgagatt tccttaacaa acagagcaga aaaaaagcaa
    21601 gtgaagagat tctaaatagc cttcaagaac aaggccatcc ctttacctaa ttttacgtat
    21661 ctgaaatata tgtaacctga cgatcacttt ttattccgtg atcaaaatca cctcttaaaa
    21721 tgcaatttag caaccgattg caataaaaca tttaaacaga tcacaaaatc acctaaaatc
    21781 gcccgctcgc ggcatatccc gctatgccat tttatttttg aacgcaggcc aatcatgact
    21841 aacagcaatc gcatcaagct cacatggatt agctttctct cctacgcact gactggtgcg
    21901 ttggttattg tcaccgggat ggtgatggga aatatcgccg attatttcaa tctgcctgtt
    21961 tccagtatga gtaacacctt caccttcctc aacgccggca ttttaatctc tatcttcctc
    22021 aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt tacgttttgg ctttctcctg
    22081 atggtgctgg cggttgccgg tttgatgttc agccacagcc tggcgctgtt ctcggcggcg
    22141 atgttcattc tcggggtggt cagcggcatc accatgtcga ttggtacatt cctggtaaca
    22201 caaatgtatg aagggcgtca gcgcggttcc cgcctgttat ttaccgactc cttcttcagt
    22261 atggctggga tgattttccc aatgatcgcc gcgtttctac tggcgcgcag cattgagtgg
    22321 tactgggttt atgcctgcat cgggctggtg tatgtcgcta tttttattct gaccttcggc
    22381 tgtgagttcc cggcgctggg caaacatgcg ccaaaaacgg atgctccggt agagaaagaa
    22441 aagtggggga tcggcgtact gtttctctct gttgcggcgc tgtgctacat cctcggtcag
    22501 ttaggtttta tctcctgggt gcctgagtat gccaaaggcc tgggcatgag cctgaacgac
    22561 gcgggcacgc tggtgagtaa cttctggatg tcatacatgg tcggcatgtg ggcgttcagc
    22621 tttattcttc gcttctttga tttgcaacgc attctgaccg tactggctgg tctggctgcg
    22681 attctgatgt acgtctttaa caccggaaca ccagcacata tggcgtggtc aattctcgct
    22741 ctgggcttct tctccagcgc gatctatacc accatcatca ccctgggttc acagcagacc
    22801 aaagtaccgt cgccaaaact ggttaacttt gtcctgacct gcggaaccat cggtactatg
    22861 ttgacctttg tggttaccgg cccgattgtt gaacatagcg gtccgcaggc ggcactgctt
    22921 acagcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct tcctgttagg tttcgtcagc
    22981 cgtcaccgtc agcataacac cctgacctct cattaattgc tcatgccgga cggcactctc
    23041 gtcgtccggc cttttcctct cttcccccgc tacgtgcatc tatttctata aacccgttca
    23101 ttttgtccat tttttgcaca aacatgaaat gtcagacaat tccgtgactt aagaaaattt
    23161 atacaaatca gcaatatacc ccttaaggag tatataaagg tgaatttgat ttacatcaat
    23221 aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa aagaaatcga ggcaaaaatg
    23281 agcaaagtca gactcgcaat tatcggtaac ggtatggtcg gccatcgctt tatcgaagat
    23341 cttcttgata aatctgatgc ggccaacttt gatattaccg ttttctgtga agaaccgcgc
    23401 atcgcttatg accgcgtaca cctctcgtct tacttctctc accacaccgc cgaagagctg
    23461 tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca aagttctggt cggcgaacgc
    23521 gctatcacca tcaaccgtca ggagaaggtg attcactcca gcgccgggcg caccgttttt
    23581 tatgacaagc tgatcatggc gaccggttcc tacccgtgga tcccgccaat caaaggttct
    23641 gatactcagg actgctttgt ctatcgcact attgaagacc tcaacgccat tgaatcctgc
    23701 gcccgtcgca gcaaacgcgg tgccgttgtt ggtggcggcc tgttaggtct ggaagccgct
    23761 ggcgcgctga aaaacttagg tattgaaacc cacgttatcg aatttgcccc tatgctgatg
    23821 gcggaacagc ttgatcagat gggtggcgag cagctgcgtc gcaaaatcga aagtatgggc
    23881 gtgcgcgttc acaccagcaa aaacaccctt gagattgtgc aggaaggtgt cgaagcgcgt
    23941 aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg actttatcgt cttctctacc
    24001 ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc tggacgttgc tccgcgtggg
    24061 ggtatcgtca ttaatgattc ctgccagact tccgatccgg atatctacgc catcggtgaa
    24121 tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac ctggctacaa aatggcgcag
    24181 gtcgccgttg accatattct cggtagcgaa aacgcctttg aaggtgctga ccttagcgcc
    24241 aagctgaaac tgctgggcgt agacgtaggc ggtattggtg atgcgcacgg tcgcacgcct
    24301 ggcgcacgta gctacgttta cctcgacgaa agtaaagaga tctacaaacg cctgattgtc
    24361 agcgaagaca acaaaaccct gctcggtgcg gtactggtgg gcgataccag cgactacggc
    24421 aacctgctgc aactggtgct gaatgctatc gagctgccgg aaaacccgga ttctctgatt
    24481 ctgccggcgc actcgggtag cggtaagccg tctatcggtg ttgataaact gccggacagc
    24541 gcgcaaatct gctcctgctt cgacgtcacc aaaggtgatc tgattgctgc catcaacaaa
    24601 ggctgccaca ccgttgcggc gctgaaagct gaaaccaaag cgggtactgg ctgcggtggc
    24661 tgtatcccgc tggttactca ggtactgaac gcggaactgg cgaaacaggg catcgaagtt
    24721 aacaacaacc tgtgcgaaca ctttgcttat tcgcgtcagg aactgttcca tttgatccgc
    24781 gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac acggcaaagg ctacggttgt
    24841 gaagtttgta aaccgaccgt cggttcgctg ctggcctcct gctggaacga atacattctg
    24901 aagccggaac atactccgct gcaggattct aacgacaact tcctcgctaa catccagaaa
    24961 gacggcacct actcggtgat cccgcgttct ccgggcggtg aaatcacccc ggaagggctg
    25021 atggcagtgg gtcgtatcgc gcgtgaattt aatctctaca ccaaaatcac cggctcccag
    25081 cgcctggcga tgtttggcgc acagaaagac gatctgccgg aaatctggcg tcagctgatt
    25141 gaagctggct tcgaaaccgg tcatgcctat gcgaaagcac tgcgtatggc gaaaacctgc
    25201 gtgggtagca cctggtgccg ctacggtgtt ggcgatagcg tcggcctcgg cgtggaactg
    25261 gaaaaccgct acaaaggcat ccgtacgccg cacaaaatga agttcggtgt ctccggctgt
    25321 acccgtgaat gttcagaagc tcagggtaaa gacgtgggta ttatcgccac tgaaaaaggc
    25381 tggaacctgt atgtttgcgg taacggcggc atgaaaccgc gtcatgcgga tctgctggca
    25441 gcggatatcg atcgcgaaac gctgatcaaa tatctcgacc gcttcatgat gttctacatc
    25501 cgtaccgccg acaaactgac gcgtaccgcg ccgtggttag aaaacctcga aggcggcatc
    25561 gattacctga aagcggtgat cattgacgac aaactggggc tgaacgctca tctggaagaa
    25621 gagatggcgc gcctgcgtga agcggtagtg tgtgagtgga ctgaaacggt caatacaccg
    25681 tccgcgcaga ctcgcttcaa acacttcatc aacagcgaca agcgtgaccc gaacgtgcag
    25741 atggtgccag agcgcgaaca gcaccgtccg gcaacgccgt atgaacgtat cccggtaact
    25801 ctggtggagg acaacgcatg agccagtgga aagacatctg caaaatcgat gacatcctgc
    25861 ctgaaaccgg cgtctgcgcg ctgttaggcg acgagcaggt cgcgattttc cgcccgtatc
    25921 acagcgacca ggtgtttgcg atcagcaaca tcgatccgtt cttcgagtcc agcgtgctgt
    25981 cacgcgggct gattgcggaa caccagggcg agctgtgggt cgccagcccg ctgaaaaaac
    26041 agcgttttcg cttaagtgat ggcttgtgca tggaagacga acagttttcc gtcaaacatt
    26101 acgaagcacg agtgaaagac ggcgtggtgc agctgcgcgg ttaatgtttt aacgggaggc
    26161 gcaatgcctc ccctttttgc atggtcctgt aatgaccttc ggtataatgc aggacatttt
    26221 ttaaactttt tgttttattt tttgttttta ttttttaaag gataatcaaa tgtttacaga
    26281 cactattaat aagtgtgcgg ctaatgctgc acgcattgca cgcctgtcgg caaataaccc
    26341 gctcggcttt tgggtcagct ccgccatggc gggcgcatat gtgggtcttg ggatcatcct
    26401 gattttcacg ctcagtaatt tgctcgaccc gtccgtacgt ccgctggtga tgggcgcgac
    26461 ctttggtatc gccttaacgc tggtgattat cgccggttct gaactgttca ccggacacac
    26521 catgttcctc acctttgggg taaaagcggg cagcatcagc cacgggcaaa tgtgggcaat
    26581 cctgccgcaa acctggctgg gtaacctggt cggttccgtc ttcgttgcca tgctctatag
    26641 ctggggcggc ggtagcctgc tgccggtaga taccagcatc gttcactccg tcgcgctggc
    26701 taaaaccact gcaccggcaa tggtactgtt cttcaaaggt gcattgtgta actggctggt
    26761 ttgcctggca atctggatgg cgctgcgcac tgaaggggcg gcgaaattta tcgctatctg
    26821 gtggtgtctg ctggcattta tcgcgtccgg ctacgagcac tctatcgcta acatgacgct
    26881 gttcgcgctc tcctggttcg gcaaccacag cgaagcctac acgctggcgg gtattggtca
    26941 taacctgctg tgggtgacgc tgggtaatac tttatcaggt gccgtattca tgggattggg
    27001 ttattggtat gctacgccga aagcgaatcg tccggttgcg gacaaattta atcaaactga
    27061 aacggctgcc ggttaattac taaggggttt ttacgtggat catttgccta tattttgcca
    27121 attacgcgat cgcgactgtc tgattgtcgg cggtggtgat gtcgcggaac gcaaagcaag
    27181 gttgctgtta gacgcaggcg ctcgcttaac ggtgaatgca ttagcgttta ttccacagtt
    27241 caccgcatgg gcagatgcag gcatgttaac cctcgtcgaa gggccatttg atgaaagcct
    27301 tctcgacacc tgctggctgg cgattgcagc gacggatgat gacacgctta accagcgcgt
    27361 cagcgaagcc gctgaagctc gtcgcatctt ctgtaacgta gtcgatgcgc cgaaagccgc
    27421 cagctttatt atgccgtcga ttattgaccg ctcaccgcta atggtagcgg tctcctctgg
    27481 cggcacctct ccggttctgg cgcgcctgtt gcgcgaaaaa ctcgaatcac tgctgccgtt
    27541 gcatctgggc caggtggcga aatatgccgg gcaattacgc gggcgagtga aacaacagtt
    27601 cgccacgatg agtgagcgtc gccgtttctg ggagaaattg ttcgttaacg accgcctggc
    27661 gcagtcgctg gcaaacaacg atcagaaagc cattactgaa acgaccgaac agttaatcaa
    27721 cgaaccgctc gaccatcgcg gtgaagtggt gctggttggt gcaggtccgg gcgatgccgg
    27781 gctgctgacg ctgaaaggac tgcaacaaat tcagcaggca gatgtggtgg tctacgaccg
    27841 tctggtttct gacgatatta tgaatctggt acgccgcgat gctgatcgcg ttttcgtcgg
    27901 caaacgcgcg ggataccact gtgtaccgca ggaagagatt aaccagatcc tgctgcggga
    27961 agcgcaaaaa ggcaaacgcg tggtgcggct gaaaggtggc gatccgttta tttttggccg
    28021 tggtggcgaa gagctggaaa cactgtgcaa cgcgggtatt ccgttctcgg tggttccggg
    28081 tattaccgca gcttctggtt gctctgccta ttcgggtatt ccgctcacgc atcgcgatta
    28141 tgcccagagc gtgcgcttaa ttaccggaca cttaaaaacc ggtggcgagc tggactggga
    28201 aaacctggcg gcagaaaaac agacgctggt gttctatatg gggctgaatc aggctgcgac
    28261 tattcagcaa aagctgattg aacacggtat gcctggcgaa atgccggtgg caattgtcga
    28321 aaacggaaca gcagtcacgc agcgcgtgat tgacggtacg ctcacgcaac tcggtgaact
    28381 tgctcagcaa atgaacagtc catcgctaat tattattggt cgggttgttg gcctgcgcga
    28441 taaactgaac tggttctcca accattaatt taacccgggc cagagaattc tggccttctt
    28501 aatgactctt tttatatatt cagcaaataa aacatagccc cttaataaat attatctgct
    28561 aatgggttat attcaccgtt acagcgactt acaaaattaa gtcattcgct cactgacgcc
    28621 aattaaatat tcctgtatcg ttcttaacga gcagtctgct tatattcata taatcaatga
    28681 atattaatta atatataata catagggaat gtaaatgaac aaatttatta aagttgcact
    28741 ggtaggtgca gtactggcta cgttaactgc atgtactggt catattgaaa accgtgataa
    28801 gaactgctct tacgactacc tgctgcaccc ggcaatttct atttctaaaa tcattggcgg
    28861 ttgcggtcct actgcacagt aagtctctga cagaaatcgg ctaacaccgg tttaattgcc
    28921 cgacgccctc tggattttcc gagggcgtat ttgtttctat gtccttcttg cccccgcccg
    28981 taaaaatatt ttcactttaa attcaatttg ataactacat ccattagcca cagcgcgatc
    29041 ctgtgcgaaa ttttgtgatc ttcctccaca ttacataaca tcatatgttg ttatattcat
    29101 catgcattgt catgttacct tttaaatgac tgcaaactct cccctacaac gtattggaca
    29161 agaaaaaggt atcgctatgg gaagccagga actccaacgc aagctcggat tttgggccgt
    29221 tcttgcaatc gccgtcggga caaccgtcgg ctccggtatt tttgtatctg tgggtgaagt
    29281 ggcaaaagca gcgggcacgc cgtggcttac ggtgctcgcg tttgtcattg gcgggttaat
    29341 tgtgatcccg caaatgtgcg tctatgcgga actatccacc gcttatccgg aaaatggcgc
    29401 agattatgtt tatctgaaaa atgccggaag ccgaccgctg gctttcctct ccggctgggc
    29461 cagcttctgg gccaacgatg cgccgtcatt gtcgattatg gcgctggcga ttgtcagcaa
    29521 tcttggcttt ttaacgccta tcgatccgtt gctcggtaaa tttatcgccg ccggattaat
    29581 tatcgccttt atgttgctac acctgcgctc cgttgaaggc ggcgcagcgt ttcagacgct
    29641 aattaccatc gccaaaatta tcccgttcac tatcgtcatt ggccttggga tcttctggtt
    29701 taaagcggag aattttgccg cccctaccac cactgcgatt ggcgcaacgg gcagctttat
    29761 ggcgctgctg gcggggatct ctgccaccag ttggtcgtat accggcatgg cctctatctg
    29821 ttatatgacc ggcgaaatta aaaaccccgg aaaaaccatg ccacgagcgc tgattggttc
    29881 ctgtctgctg gttctggtgc tctacaccct gctggcgctg gtgatttccg gcctgatgcc
    29941 cttcgacaaa ctcgccaatt ctgaaacgcc gatttccgac gccctgacct ggatccccgc
    30001 actcggcagc accgctggga tctttgttgc catcacggcg atgatcgtca ttcttggttc
    30061 gctttccagc tgcgtgatgt accagccgcg gctggaatac gcgatggcga aagacaacct
    30121 gttctttaaa tgcttcggcc atgtgcatcc gaaatacaac acgccggatg tctccatcat
    30181 cctgcaaggg gcgctgggga tcttcttcat cttcgtttcc gatctcacca gcctgctggg
    30241 ttatttcacc ctggtgatgt gtttcaaaaa taccctcacc ttcggctcca tcatctggtg
    30301 tcgtaaacgc gacgattaca aaccgctgtg gcgtactccg gctttcgggc tgatgaccac
    30361 cctcgccatt gcgtcaagcc tcattctggt cgcctcaacc tttgtctggg caccgattcc
    30421 cggccttatc tgcgccgtca tcgttattgc tactggtctg cctgcttacg ccttctgggc
    30481 gaagcgtagc cgccagctca acgctttgtc gtaatgttat ctggagaaaa taaaatgttg
    30541 gatattgata aaagcaccgt ggactttctg gtcaccgaaa atatggttca ggaagtggaa
    30601 aaagttctca gccatgacgt tccgctggtg cacaccatcg tggaagagat ggtgaagcgc
    30661 gacattgatc gtatttattt cgttgcctgc ggatcgccac tcaacgcggc gcaaacggcg
    30721 aaacatctgg cggatcgctt ttccgatctt caggtctacg ccatttccgg ctgggagttc
    30781 tgcgataaca ccccgtatcg cctcgacgat cgttgcgcgg tgattggcgt ttctgactac
    30841 ggtaaaacgg aagaggtaat caaagcgctg gagctgggcc gggcctgcgg cgcactcact
    30901 gcggcgttca ccaaacgcgc ggacagcccg attacctcgg cggcggaatt tagcattgat
    30961 tatcaggccg actgtatctg ggaaattcac ctgctgctct gctacagcgt ggtgctggag
    31021 atgatcaccc gcctcgcgcc gaacgcggaa atcggcaaga tcaaaaacga tctcaagcag
    31081 ttgccgaatg cgctcggtca actggtacgc acctgggaag aaaaaggccg ccagcttggt
    31141 gaactggcca gccagtggcc gatgatttat accgttgctg cgggtccgct gcgtccgctg
    31201 ggttacaaag aaggcattgt aacgctgatg gaatttacct ggacgcacgg ctgcgtgatt
    31261 gagagcggag agttccgcca tggcccgctg gagattgtcg aaccgggcgt tccgttcctg
    31321 ttcctgctcg gcaatgatga aagtcgccac accaccgaac gcgccattaa ctttgttaaa
    31381 cagcgtactg acaacgtgat cgtcatcgat tacgccgaaa tttcgcaagg gctgcacccg
    31441 tggctggcac cgttcctgat gttcgtgcca atggagtggc tctgctacta cctgtctatt
    31501 tacaaagatc acaacccgga tgaacgccgc tattacggtg gtctggtgga atattaatcc
    31561 ctctcccggc ccggcaacgg gccgaattta cgcaaggagt tacccgatga aaacaggtat
    31621 gtttacctgc ggccaccagc ggctgcctat tgaacacgca tttcgtgatg caagcgagct
    31681 gggttacgac ggcatcgaaa tttggggcgg tcgcccgcac gcgttcgcgc cggacttaaa
    31741 agcgggcggc atcaaacaaa tcaaggcgct ggcgcagacg tatcagatgc cgattatcgg
    31801 ctatacgcca gaaaccaacg gctatccgta taacatgatg ctgggcgatg aacatatgcg
    31861 tcgcgaaagc ctcgacatga tcaagctggc gatggatatg gcaaaagaga tgaacgcggg
    31921 ttatacgctg atttccgcgg cccacgcggg ctatctcacg ccacctaatg ttatctgggg
    31981 acggctggca gagaacctaa gcgaactgtg tgagtacgcg gaaaacatcg gcatggatct
    32041 aatcctcgaa ccgttaacgc cgtatgaatc gaacgtcgtg tgtaatgcta atgatgtgct
    32101 tcatgcgctg gcgctggtgc cttcgccgcg cctgttcagc atggtcgaca tttgcgcgcc
    32161 gtatgtccag gcggaaccgg tgatgagtta tttcgacaaa ctgggcgata aattacgtca
    32221 tctgcatatt gtcgacagcg acggggccag cgacacgcat tacattcctg gagaaggcaa
    32281 aatgccgctg agggaactga tgcgcgatat tatcgaccgg ggctatgagg gttactgtac
    32341 ggtggagctg gtgacgatgt atatgaacga gcccagactc tatgcccgcc aggcgctgga
    32401 acgctttcgc gcgctgctgc cggaggatga gagatgaaaa ccctggcgac aatcggcgat
    32461 aactgcgtcg atatctaccc gcaactgaat aaagcgtttt ctggcggtaa tgcggtcaat
    32521 gtggcggtgt actgcactcg ctacggcata cagccgggat gcattacctg ggtgggtgac
    32581 gatgactacg gcacaaagct gaagcaggat ctcgcccgca tgggcgtcga tatcagccat
    32641 gtccatacga aacacggcgt taccgcacaa actcaggtgg aactgcacga caatgatcgc
    32701 gtttttggcg actacaccga aggcgtgatg gccgactttg ccctgagtga agaggattac
    32761 gcctggctgg cgcagtatga cattgtgcac gcggcaatct ggggacatgc ggaagacgca
    32821 ttcccacagc tgcacgctgc gggcaaactt accgctttcg acttctccga caagtgggac
    32881 agctcgctct ggcagacact ggtgccgcat ctcgattttg cctttgcctc cgcaccgcaa
    32941 gaagacgaag cgctgcgtct gaagatgaaa gcgattgttg cccgtggcgc aggcgtggtg
    33001 attgtcacgc tgggtgaaaa cggcagcatt gcctgggatg gcgcgcagtt ctggcgtcag
    33061 gctcctgaac cggtgacggt tatcgacacc atgggtgccg gagattcgtt cattgccgga
    33121 ttcctttgcg gctggtctgc ggggatgaca ttaccgcagg cgatggcgca gggaacggcg
    33181 tgcgcggcga aaaccattca gtaccacggt gcctggtagg tataacgttg gcgtgagcat
    33241 cttcacgcca acgtgctgtt acttgccgga aaacgaccct ataatccgag taattcattc
    33301 tttatttcag ggtcgattat gtcagctacg gaccgctact ctcatcaact cctctacgct
    33361 accgtccgcc agcggttgct ggatgatatc gcgcaggggg tttaccaggc cgggcaacag
    33421 atccctaccg aaaacgagct ttgtacgcaa tataacgtca gccgcattac tattcgcaaa
    33481 gctatcagcg acttagtggc agacggcgta ctgatccgct ggcagggcaa aggcaccttt
    33541 gtacaaagcc agaaggtcga aaacgccctg ctgaccgtca gtggttttac cgattttggc
    33601 gtctcacaag gcaaggcgac gaaagagaaa gtgatcgagc aggaacgagt cagcgccgcg
    33661 ccgttttgcg aaaagctgaa catccctggc aacagcgaag tgttccatct ttgccgggtg
    33721 atgtatctcg ataaagagcc gctgtttatt gatagttcct ggatcccact gtcgcgttat
    33781 cctgactttg atgagattta cgttgaagga agctccactt atcagttatt tcaggagcgt
    33841 ttcgacacgc gagtggtcag cgacaaaaag accatcgata tctttgccgc cacccgcccg
    33901 caggcaaaat ggctgaaatg cgaactgggc gaaccgttgt ttcgcatcag caaaatcgcc
    33961 tttgatcaga atgacaaacc ggtgcacgtc tccgaactct tctgccgcgc caatcgcatc
    34021 accttaacga ttgataataa aagacattaa ccgtaggccg gataagatgc gccagcatcg
    34081 catccggcga tgctggcgcg ttgaatttta catcccgtac gttcccctca ccctaaccct
    34141 ctccccaaag gggcgagggg accgttcagt acggaattta gcttgacggt gctatccagc
    34201 attaaatact ggcaatactc tcacgcaaaa tccgcagcac cgtctcttca ccgctgcgat
    34261 tcgggttaat gcgaatcgca caatgttctg attgtggatt cgcctggcga aacgttccgg
    34321 aaaggcgata aaagagcggc gggatttcat atttcgactc tgcacccacc gggtaaggca
    34381 aggcaccgcg cttttgcgcc tcttccagca ctctggcggc aatcggctga tgaaactcga
    34441 caatcaacac cttcgactgc gcattagcaa tcaccgcgct tttcacttcc ggcaccgcac
    34501 caccgttaag caaagccagc aaccgttcag atacccctgc ctgcaccgcg tgcatcactg
    34561 gcgcaaacac cagaccacgc aatacttcca gcgcctgtgc gccctggatc tggctaccgc
    34621 cggagtaaag cgtggcgcga atacggttga taacatcagc atcgccgacc actgcaccaa
    34681 caccctctgg cccaaatagc ttgaagcagg aaaatgtcga gacattcgcg ccgcattcac
    34741 agccgattcg cgccaccttc atcaccgcat agttgtcatc ggttaacgct ggaacacctg
    34801 ccgcgcgcaa cgttgccagc acatctgcca gcacgtagct gtcctgcggc tgctggcgcg
    34861 tatgctgcac cagcgccgca tccggttgtt gctcgtcgac gacctgcttc agtgccgaca
    34921 ggtcattgaa atcaacagta ataagcgtca gccccatctg ctcaataata acccgtgtcg
    34981 tcgggtaaac aggcgcgtca tgcaccagaa gacgctgccc cggtttgagc aaagccgcca
    35041 gcccggcgcg aatcgcgcca gtccccgcgc cctgcaccag cgccgcagcc tgtgcgtgaa
    35101 atgcatcagc cagcacctgc tccacccgtt gggtaatacg cggttgattc agacctggag
    35161 ttaaccctaa atcaccgcca gtaagaaact cgctgccggg gaaatggcga caaatgctat
    35221 ccaccagcgc aaactgcttt tgctgcgcct caataatcgt caggctttgc agaggaaacg
    35281 tcttcatagg tgccttcctt aagccggaac gaacaggcca agccagtaaa gaacattcag
    35341 cagaataccg gtgatcatta ctgccacgac cggtgccgcc attttctgta ccggacggcc
    35401 cagggattcg ttgaggaagt aaatggcaac cgcgatagag aatccggtat aacccgccat
    35461 cttaattgct gcgaaaatcg aaccgaccag cagcgccact tccatcagca tattcatggc
    35521 gttacggatg ttatccgacg cattacgcac cgacgggtag cgtcccagcc atttgccgat
    35581 cgaacgaagc agcaagactt ccgccgaaat aaccactgcg cctaataccg ccgcaaccat
    35641 cggatttggc gagagatagc ccaccgcata aacaaaggta aagcccgcaa ctgcatacac
    35701 gccagtggct aacgcggtgg tggcaatcaa cggcacaaaa cccagtccgc gcataaattc
    35761 tgccagagcc gcctgattaa tcagcgtttg cgattgttcc ggcgtcacgc ctgcggaata
    35821 cgctttctcc agtgtgaaga tcgaaacttc gctaccagca aaaatcttca tactggcaac
    35881 ggcggcaatc aatgctccca cgatggcgat ataaggtaag tttttgataa tccgcgacgt
    35941 gcgctcttca aacaccgaaa gcccgctggc atcgatgtca ttttcatcac gatgacgcag
    36001 gtcgtgagtt atcgcgatcc ccagcagcat caccatgcca ataaagattt cgatggattc
    36061 agggttaaga tgtgggaaat agcgcacgac taccacacgg gtcatcagaa caaccacggc
    36121 ggcgaccaga ctttgcttcc agccaaactg gtagaaaatc gccaccagcg ggaacagtgc
    36181 aaaagcagaa accaccggcg agcttaattc cccgaggcta cctaatacat ccaccggcag
    36241 cgcggtcagc agctggttta ctggcaacag gcaagtaagg atcaacacgc cccagatagc
    36301 gccaagacca aacgccatca ggctgtttat cgccagtacg ccgaggatat cggtaggaag
    36361 aaagagtaac catgcgttga gcagcccggt tttcagggta aaagagatcc ccaccgaggc
    36421 cacaaaacca atgctcagac caaaagcgat actccccgcc tcgcgacgat tcatatagcc
    36481 ttcaatcagt tgcggcagga tcgggcggat gccgtcatga aaaacagccg ccgagcgatg
    36541 cgccagaagc gatgtcatac ccgtcaggca cgccaccacg ataatctgaa tatacagatc
    36601 catagtcgtg ccttttattt cagatggtta atcagcatcg gaatcgcgtg ttcgacgtgc
    36661 tcaacggaaa ggccaaacgc cactttccct tcagcgatca ttttggcgat atgctcgtct
    36721 ttcgctttaa tgcctggttt ggcaatggtg caacttttgt tatagccaat caccgcgatg
    36781 gcaatcgaca atgccgcgcc cgcaccggta ttacacgcac cgatgtagta atccagttgc
    36841 ccggatttca ctttcattgc cgcttccatg tcgttgtgaa tgaaaacttc aaaactgcca
    36901 ggagccgtcg cttcaatggt ttttttaatc tgctcacgct gtaagcctgc aacgccaatc
    36961 tttttcatga gtctgtcctt attggaaaaa ttgagaggga ttttcacgta acatcacatc
    37021 cacatcggcc tgactgaatc ctgactggcg caattgcgga ataaaggtgg tcaataaata
    37081 gtcatagcca taaccaccat tggcttttaa atgggagcgg cgcgtaatat ccatcgacag
    37141 catgacgcgg ttcagcaacc cacggtcacg tagcgcgtgg agcatcgcaa tgcgcttttc
    37201 gtccgggtag taactgttct taccaatggt gtcgaactgc acgtacgcgc cgagatcgat
    37261 catcttcaaa atgttgtcga gattgtcttt cagatcgcag tgaccaacgg tgacgcgcga
    37321 aagatcaact ccgtgggctt gcaacagagc cagttgttcc agccccatgg tgctgaacga
    37381 agtatgcgtg gagatcgggc gtccggtctg gttatgcgcc agcgcagcgg caataaacac
    37441 cttctcttcc agcggcgtaa tcttcccttc gctagagccg atctccgcga taatcccggc
    37501 tttcaggtcg gtgccatcga taccctgttc aatttcatcg accatctcct gcgccagttc
    37561 ctgcacgctg cgggtcgcca catgctccgg gaaaaaagcg tcctggtaat aaccggtgca
    37621 ggccaccacg ttgatccccg tctcgcgcat tacatcgagc ataaattgcg cattgcgccc
    37681 catgtaacgg ttggtcatct caatcacatt acgcacgccc cgggtcatca ggtcgttcat
    37741 ctcctggcaa atgaacgcat actgatcaag gcggcagtcc acgttgtttt taaagccgga
    37801 gagatcaata tgcagatgct catgggccag ggtgtaaccc gtcggatcaa tactcatagg
    37861 gtgtctcctg caaaccggag ggaggaaaga aaagagcgac cattttgtgg cggtggcgca
    37921 cggaaaaatt cacacacggt agcccccaca tcagaaagcg tggtgcgtac accgagttgc
    37981 gtgtggacca gcccttgctg ataaaccagc actggcacca cttcacgggt atggtggctg
    38041 tgaccaatgg tcggatcgtt accgtgatcc gccatcacga ccaggcaatc atctggctgc
    38101 atcgcctcaa caagccgggc aaggttacgg tcaacgacct gcaaacgttc ggcataacgt
    38161 gcgacgtctt ctgcatgacc agcgaggtcg gtttcctgga tattgatgca aataaacgcc
    38221 gtcggatgcg tgttaaattc gtcgagggtg atatccataa tccgctggct atccaccagg
    38281 ttttgccagc tcacgccata aggattgctg acgatatctg ccaccttacc taccagcacg
    38341 gttggcacgc ccgcctcata cagtttttgc ggcacctgca ctttttcatc aacaccgtag
    38401 cccatatgca cgacctggaa gccgttgtca taagcgccag aacgcggcgc attaatacca
    38461 ataaagcgcc cttctttgct ttctgcggca tcgagaatac gctgactgtc ggttaacaag
    38521 ccgccaaatg taatgacccg accgacctgt acctgctcac gcacgatacg accaattttg
    38581 atcgcgtcgt caaaagagat cacagagaga ttggcggtaa tgttatagac ctggcctaaa
    38641 tccgcctcga gattatcgcc aatcgcaacc gcctgattga cccacagaaa ttgcagatca
    38701 tcgccacggc gctccacctg ccagccagcg gaaactaatg cctgctcaac acggccaatc
    38761 acatcgcgaa agggcatccg cagcggcggt aacgggcgcg tgcctaaaat ttcctgatgc
    38821 cccataaagg tatcgccacc ttcatgttgc agctctgcca ccccccaggt tgcggaatct
    38881 gacggttgca tatcacctgg tgcataaccc aatgcgttga ttagccccag cttctccagc
    38941 gctggtagct gcaaatgcgg caactggctc aggatgtgac cacaggtatt cgccccggca
    39001 tcttgcgggc gcaccagcgt gacatctttc attgccccta cgccaaagct atcaatcact
    39061 aacaccacaa atcgcgccat tacgccccca gagagtttcc aagactgtca taacgaccga
    39121 cgattttcgg ttcgccacgg tgaataccgg acaccagcac cacatcgcta cgagtgacaa
    39181 aaatctgcgt gcgaaaacag agcactactg cactgcttac cggaaactcg cctgccagcg
    39241 gcagggtgta gtcgatactg ctgtcatcca cagttttgag attggtttca gtaatctttt
    39301 gattttctgg cgtaaacacc agcgcatgtt gcgcatgacc acgacggtag taaccgccgc
    39361 cgtagcagta gctgtcgcca cggaaatgat gggagatttc acttaaccaa agcatcgcta
    39421 tatattcagg ctgatcgccc tgctggtttg ccggaatagt gcccgtcagc gcatgaccgg
    39481 gttcggcatg agtcacaccg tattccgcca gcatcggcag cgaagtgcag ctggtcgcag
    39541 aaggcgcgtt cagttgctca atcgcaatac cagatttcgc cagttgatcc cgtgcttgta
    39601 ccagcgtgtg aagattcggt gtcggcaaaa ctttcccggc agcctcatcc caaagcaggc
    39661 aagggaaatg ggtaagtccg gctaaatgca gacccggcag tttcttaatt tcagcaacca
    39721 cctcgtgcag cgaatgctgt acaaaaccgc tctcctgccc cggataaaga aaatcatcgt
    39781 cgctataaac tttgagcagc acaaactgca cacgtccggc cttcaccgct gccgcagaaa
    39841 cttcccgcgc tttgtcgaga gtaaacacgg tgatgacgtc ggtgccctgt tcaacggcgt
    39901 cagcaacctg atgacaaggg atttgtacca gatgcccctg atgcgccacc gacaaaccag
    39961 cgcggcgcat gactcgcgcc tctttgtaat ccaccgccac aatgccgctg taacctaatg
    40021 ccagcaattt ttccgccagc catgggttac ggccaaactg cttggtcatc agatacagtt
    40081 caatgccgta aagccgcgcc gtctcaatca gtcgcttacc gttttccagt acctgatcca
    40141 cgtcgatcac ccagctgtcc ggggcgatct tgccctgctg ccacaaactt agtgcggcgg
    40201 aaatcagcgc cgggttctgg cgtttcaatg cttctacaaa catcttgttg cctcgtttct
    40261 gttccttgaa ttaaatattc atttttttga atatttaggt tgtgcgacgt ggatttgtcg
    40321 ggagcttcaa acctcttcgt tagcagccat ccataatcca taaaggttgg ccagcaaata
    40381 gccttcttca cacgggttaa cttccagtgc gaattccttc aacaacacct gatgcaattg
    40441 cacaacggct tgccagtggc tggattgcgc cagctctgcc agcaattcgt tatccagcgg
    40501 ctctatttct tcaccacggc gactgcgcat cagtgcactc gccatatgtg tcatcgccat
    40561 cgttccttgc tcactgcgca ccggcagatg gcactctgtt tccagtacgt tgacgacctg
    40621 catcatgcct ttgcagatgt ccttatcaat aacgcctgcc tcgcaaagca ggttgagtct
    40681 ggtttccata tcagatgcct ttcgtttaat aactcggttc ctgttcccca ctcactaccc
    40741 gttgttgatg ggcgagcagg gcgtgtttat ctacaacggc acgcagcagt tgttgcattg
    40801 ggtaatcatc gactcgcgtc agaaccacgg cttcggtggc ctgtaaaaat cgcggatcgt
    40861 ctgtgagcgg cgtcgcctct aatcccagca tggtcagttc gttttccgcc accacgttcc
    40921 agatcaccgc atcgacatcg cctttaacaa tgcgttgtaa actctcgtga taagagagat
    40981 cgacacgttc cacatcacta tcgccaaaaa agacatcggt catgattttc tgatccgccg
    41041 aacggttatc cagccccacg cgcttcacgt ttgcggactc gcctttacgg caaatcaact
    41101 ggtgctcgcc aacgtaggtg tgcggcccca actccagcgc gaggcaaaac cctttttgcg
    41161 tgagataact ttccgccgcc agtcgggaaa ccaccgccat gtcatacacg ccgttaagta
    41221 aacactccac gcgaatatcc gcgccgcgca tatgcgcata gtaaaaagga atgccatcaa
    41281 actgggcttt caatccgctc gctaaacctt cgtacaagcg ggtatagggc aaaggcatcg
    41341 cacataccac gttgttgata tccacatgac tcagcaatgc tttgttatcc atctcgacca
    41401 gataactgcc attgcgccca cggcgttcaa tccgtatcgc tccgcttgat tccagcgttt
    41461 tcaacgcggc ctgcgtcagg ccaacggatg aacggcattc agttgccagc tcatctatgg
    41521 ttttcaacct attaccgcat ttttcaccca acaaataacg ggccagcgtc gtgataacga
    41581 cgccttcttt tttgataaac gttcgacgca taatatattt tcaataaaat gaatgtttat
    41641 atcttcatta aaatgaagat aagtggcaac tgtgagatgt ggaaacggcg aggcgcttca
    41701 cattttttcg gattattgca aatgaggata aaaaaaccgg gtttccccgg tttcagagtg
    41761 atgacgaagt gatttattcg acaatgcgag caaagggtaa ttcccactgg cgcttgcctg
    41821 ctcccctcac cctaaccctc tccccaaagg ggcgagggga ccgtccgagc gttttgatat
    41881 tacggcttcg ccacaaaacc aatcgcttcg tacaccgctt tcagcgtacg ggaagcgtgt
    41941 acactggctt tttctgcgcc gtctttcatc acctgttgca ggaaggcttc atcgttgcgg
    42001 aaacggtgat agcgttcctg caattcagtc agcataccgg aaacggcatc agccacttca
    42061 cctttcagat gaccatacat cttgccttcg aactgttttt ccagttctgg aatgctctgg
    42121 cccgttaccg cagaaaggat atccagcagg ttggaaacgc ccgctttgtt ctgcacatcg
    42181 tagcgaacta ccggcggctc gtcggagtca gtgaccgcgc gtttgatttt cttcactacc
    42241 gatttcggat cttccagcag gccgataacg ttattgcggt tatcgtcaga cttggacatc
    42301 ttcttggtcg gctccagcag cgacattacg cgcgcgccag atttcggaat aaacggctcc
    42361 ggcaccttaa agatctcgcc atacagcgcg ttgaaacgct gggcgatatc gcgactcagt
    42421 tccaggtgct gtttctggtc ttcacccacc ggtaccagat tagtttgata cagcaggatg
    42481 tccgcagcca tcagcaccgg atagtcaaac agaccagcgt tgatgttctc ggcataacgc
    42541 gcagatttat ctttaaactg cgtcatgcgg ctcagttcgc cgaagtaggt atagcagttc
    42601 agtgcccagc ctaactgcgc atgttctggt acgtgggact gaacaaaaat ggtgcttttc
    42661 tcaggatcga taccacaagc cagatacaag gccagcgtat ccaacgtcgc tttacgcagc
    42721 ttctgtgcat cctggcgcac ggtgatcgcg tgttggtcaa cgatacagta aatgcaatgg
    42781 tagtcatcct gcatgtttac ccactgacgc agcgcaccca tgtagttacc aatggtcaat
    42841 tcacctgagg gctgtgcgcc actaaaaacg atgggcttag tcatttttcg attcctgatt
    42901 ttcgctatgc ggaagcccta atgcgggcag aaggtcattt atagactgat aaattacatc
    42961 aggctggctg agatcgatag cctcgccgta gttatatccg taggttaagc caactgatgg
    43021 gcaacctgcc gcttttgccg cctgaatatc attgcgtgag tcgccgacaa acagcatctg
    43081 ttgtggggca attcccatcc gctcagccac cagtaacagc gggtccggat gcggtttttt
    43141 gttttgcaca tcatcgccgc cgataaccac gctaaagtat ttggcaatat ctaaggcttc
    43201 gagcagcggc gcgacgaacg gcgtcggttt gttggtgacc aggcctagcg gcaggccttt
    43261 agcctgcaac gcgcccaacg tatcggcaac gtgcgggaac aaaaacgtcc cctcttcggc
    43321 aacctcgcca tagtagcgat cgaacagttt acgcagaata cgtacctgtt cttctgccgg
    43381 aatgtcgtca tcaacgggcg gtttacccat tgttttacgc tgagtcgcac gttcctgacg
    43441 cgcccaggtc aatgcgcgct ccatcagaac atctgcgccg ttaccaatcc aggtaataac
    43501 gcgttcttca cctgcgacgg gcaactccag cgcatacagc gccatatcta ccgcagcagc
    43561 aagaccggga gcactgtcga ccagcgtacc atcaagatca aaagcgacgc cgcgaatatc
    43621 ttcaaactta ttcatgactt aactttgcca gttcactgcg catttcatca atgacttttt
    43681 tgtagtctgg ctggtcgaag attgccgaac cggcgacgaa catatccgcg cccgccgcag
    43741 cgatttcgcc aatgttgttc accttcacgc caccgtctac ttccagacga atgtcaaagc
    43801 cagactcgtc gatacgacga cgtacttcgc gcagtttatc cagtgtttga ggaatgaacg
    43861 actgaccgcc gaaaccagga ttgacggaca tcagcaggat cacatccagc ttatccatca
    43921 cgtaatccag atagctcagc ggtgtcgccg ggttaaatac cagtcccgct ttacagccat
    43981 tttctttaat cagttgcagc gtgcggtcaa catgctcgga ggcttctgga tgaaaggtaa
    44041 tgatgctggc accagcggca gcgaaatcag gcacaatgcg atcgacaggt ttcaccatca
    44101 ggtgtacgtc gataggggcg gtaatgccgt agttacgcaa ggatttcagc accattggcc
    44161 caatcgtcag attgggaaca tagtggttat ccatgacgtc aaaatgcacg acatcagcgc
    44221 cagctgccag ggcttttgcg gtatcttcac ccaggcgggc aaaatcagcc gacagaattg
    44281 agggggcaat caaatactgt ttcatccgct tctccttgag aattattttt tcgcgggtga
    44341 aacgactcct ggtttgtaca aagccagcag ttcgtccacc tttttacgtg tgccgccgtt
    44401 gctgcttata ctgcgtcgaa ctttgacgac atgcaatttt gcgcgctgat accactcacg
    44461 cgttaacatc gtatcgtgat tggagatcag cactggaata tggcgctcaa ccagaccttc
    44521 ggcgatctcc gccagatgcg cttgttgttc aagcgtaaaa ctgtttgtgt gatacgccgt
    44581 aaagttggcg gtcgcagaca gcggtgcata aggcggatcg caatagacga cggatgcatc
    44641 atctgcgcgc gccatgctat cggcgtaaga ctcacaatag aaaaaggcat tctgcgcttt
    44701 ttcagcgaag tgatacaact ctgcttccgg gaaatagggt tttttgtagc ggccaaacgg
    44761 cacgttaaac tcaccgcgca gattgtaacg acacaggccg ttgtaaccgt agcggttcaa
    44821 atataaaaac agtaccgccc gacggaacgg atcctggctt ttgttgaact cttcgcggaa
    44881 ctgatagtag acctcggcgc aatttgtttc gggaacaaac agctcgcgtg cggcctgtac
    44941 gtactcatca gtacgcatct tcacaatgtt atagagactg atcaggtcgc tattgatatc
    45001 ggcaaggata taacgagaaa agtcggtgtt gagaaacacc gacccggcac ctacaaaagg
    45061 ctcaaccaga cattcgccct tgggcaaatg ccgtttaata tcatcaagca ggggatactt
    45121 gccccctgcc cacttcaaaa aagcgcgatt tttcttcatg ctgactaact aattacacct
    45181 tctccggctg tggagaaagc tccgacagca tccagcgctt caggcaactc ccgcaacgat
    45241 aacccattgc gggagataac cttgattact tcagatcggc ctgtacctga cgcagcggtt
    45301 tcgcccacgg gtttttggcc tgaacatctg ctggcaatgt agataccgct tttttcgcct
    45361 cttctttcga agcgtacacg ccagaaacca ggacatacca cggctgacca ttacgcgtcg
    45421 tttcatagac aacgtagttt ttcagattct ctttcttcgc ccaaccgttc aggttgtcgt
    45481 agttagagga actgctcagc tgcagagtgt aatggctgga cggtgccgat ttcaacgaac
    45541 caacattacc tgcggtcttc cctccagcgg ctggtgttgc cgtggtttgc gccggggatg
    45601 ccgtctgtac tggagccgta gtcgccgtct cttttggtgc tggcgcagaa gtcgcagccg
    45661 gtgccttcgt gctcgccaca ggagcagctg gttcagtacg cttcggcgtc tgtgctaccg
    45721 gcttcggttc cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac
    45781 gagctggacg cgtagtggcc ggacgttcag cagtttgcgt tttcgccgtg tcacgcgatg
    45841 cattgccatt gcgtactggc gcgacagttg ctggttcagt cggcaatgta gaattgaccg
    45901 ccacattgtt caactgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct
    45961 gcacttcaac acgttgttga ccatccgttg ccgccggggt ttgcccttga gtcggcgtag
    46021 aagagatcgg tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg
    46081 ctggctgcac cccattcgcc tgatcggtcg cattgcctgc aagatcaata ctcttctcgc
    46141 cagacgcggt ttgatcgctg gaagaggtcg agggggcttt tagcgcagaa ccgataccga
    46201 tgatcaacag cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg
    46261 gtttactggc tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg
    46321 tttcatcttc ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt
    46381 gcgcacgagt cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct
    46441 gcggttcgcc acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac
    46501 gacgatcgct gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc
    46561 ccactaaaag gttaatgctt accacattgc aattaacctg aagctaatag accgcttgat
    46621 aagcggcctg acctttcttg ttgttacgct gattgactat cggcaatggc gttaagaaca
    46681 agctcgtgcg aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag
    46741 cgtatctctc ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg
    46801 gacatttcgc gcggcccatt gaccggtaac ccagcccgct tgagcagggt tataatacgc
    46861 tgcgtttcgg cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata
    46921 cccgcagcga ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca
    46981 tgaccaaagg tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg
    47041 gcgacaactt ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg
    47101 tccagacgca acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga
    47161 ataatgccgt atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt
    47221 ttcagacagt cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt
    47281 ttaccgaggg gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt
    47341 aacgtcgtcg ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg
    47401 gtcagatcgc ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc
    47461 ttttgtaaca acgccgtaaa gacggtatcg agtacagcca ggcttttata ctgctcgccg
    47521 tcagggagga taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta
    47581 tcgagataca gaggagccag ggtttcgttg gtgaccaaca tgacctgctc gcccgatttc
    47641 agcggtaaga atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta
    47701 cgttccccga gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac
    47761 ccgcagacga gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt
    47821 ttgcaaccac tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat
    47881 acagcggatt gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt
    47941 gcagcaacgg gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt
    48001 caagataaac gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag
    48061 agccgccgcc agtagccagc acaataccct gtttctcggt caactcattg atgacctttt
    48121 cttcgcgatc gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc
    48181 cggttcgttt ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta
    48241 actggcgccc aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt
    48301 tctctgccat tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca
    48361 gagagcgccg caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa
    48421 ttatctcaat actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct
    48481 ggtcgctgca agcgcgctgc cttgctacca ccgctctggc gataaatcac cgggtaagat
    48541 tagcgtaaaa aagacagcaa aatgccgcct gaatgataaa tcatcatcat ggaacgcagg
    48601 cagcgaagag caccaactct caaatcggta ctccttgtat gctaaatacc tgcgcgcgtc
    48661 aaatagatga aacacgttca gcataaaaac attaccgctt ttacggctgt ttactcactg
    48721 gaaaccagtc gtggcgtgat aaacaccact aactcgcgtc gttcatcttc ttttccgtca
    48781 tgacgaaata attgcccgaa ccagggaatg tcgccaagca acggtacgct atcctgaccc
    48841 gatttatttt tacgggtaaa aatgccgccc agcgccaacg tttctccgct tttgacctcg
    48901 acctgcgttt cgatctcctg cttatcaatc gccagcactt cgccatcggc ctgctgtagc
    48961 acctgccccg gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc tttttgtaac
    49021 accgtgggcg tgacctccat ccccaggacg gcctctttaa attccaccga cgtcgcgcca
    49081 ctttccccgc tggaaacctg atatggaatt tcgctcccct gtttaatgct ggcaggctga
    49141 agatgtgagg ccagcagacg cggactggcg ataatatcca gctgctgttt ttgttcgagc
    49201 gcagaaagct caagatccag caagcgtccg ttgatgcgcc caatgttaaa accgacatgc
    49261 gttgtcgccg tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc aacgccacca
    49321 gcgtgttgcg catcggccag cgtccatttc acgcctaact cacgcaaact tttttcatta
    49381 atggtgacaa tatgcgccga cagctcaacc tgcccgaccg gcagatccat ttgcgctacc
    49441 cactgttcaa gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg attggtgcgt
    49501 ttatcgacgg tcatactccc tttggcactc agtagcttct cccccgcttt cgccagttct
    49561 cccgcgtcgg cgtattgcag ggttatactg cgattttcca gcggcagatt tgcctgcgcc
    49621 cgcgcctgct ccgcctcctg gcgggcgata ttgttattct gccaggcaat ggaatgcact
    49681 gagagaatgt tgtcttcctg ccgcgttatc agtccggcgc ttttcactac agtttgtagt
    49741 gcctgcttcc agggaacatc tgttagatgt aacgacaccg taccgctgac gtctggcgac
    49801 acgaccaggt tcaacttctc ctgttcagcc agcgcctgca acacctgagc taccggaacg
    49861 tcatccacca tcagcgtcac tttttgcggc tttgccgcct gtacgccggg tatcagcatc
    49921 aacagtagtg cggctatcca ttgcttcatt tgtatctcct tgccgttgcc acaaccattg
    49981 tggcggttcg cagtttgtcc cggttcccag cgttagtgcg tctggcgtca gctgtaaaat
    50041 tgtccagccg ttttccagca catcgttttg ctgcacccgt cgccatttct tttgcccgtc
    50101 ttttattaca ccgatgatgt gctcgcctcg ccctaccatc ccctgatagc gccactggct
    50161 aagttcgcta atccggcata gatcttccgg cggtttaaaa gggtcacgca taccggttaa
    50221 aaggcacaat gcaatacctg ccaacaacca gcgtttaacc ctcatgcggt gtctccagtt
    50281 gtagcgtgaa caaaagatca tcaccttcca cgcttaacga aaaacggctc acgctgacgt
    50341 tgcgctctgc cagccgtgta aatgccgacg gcactgcttc ccacagcgtt ttcaacgcca
    50401 actcacctcc ctgcgcggat ggatgccagg aaaccagttg cgcgccgggc aactgaaaat
    50461 ccagtggcga aaagggcagc gttttttctt cgctgaaggg agtggtgtca accaggcgat
    50521 acagtgtggc ccactgctgg tgatgacttg cccgtagtcg aattaatgcg tcgcgctctt
    50581 catggtgtgt cgatgacaga aaaatgagcg taactaacat cagcaaccag aatgcccagc
    50641 aaaactggcg gaggcggggt gatgtggcga accaccagtc aaagaacatg ttcatcgcta
    50701 accttccttg ttaactgata ctcaaattgc cagcgtccct gcgcatcctg ctgcgtggct
    50761 ccccgctgat tgagatgaaa agacgcatcc tggcggagtg acgtttctag tgcgtttaac
    50821 gcggtaatgc ttgttgtcag ccccttgatc tccagcgttc cctgctgcca gcttatcgtt
    50881 gtcagccagg cgtgctcggg taaaagcgcc gccagtgctt ccagcgcaga ttgccagtcg
    50941 cgggtaaatt gtcgctggcg ctgccgttgc gaacgctgtt cgcgtaactg ctgttgctcc
    51001 agcaaacgtg gcttcgttat ctgtaagctg cgggcgagtt gttgttccgc ctgaagcaaa
    51061 actgcgtcta tgcgcgcttc ggcgctgcct gtcagacgca gtattagcgt tatcccgacg
    51121 gccagcagca gaggcgcaac gaacatcagc aaccagaaac gcagaaaagc ggtccggcgt
    51181 tgctgtcgcc agggcaaaaa attaattggc gggttcatca gtacgccttc ccaagcgcca
    51241 gccccagcgc gatggcaaag tctccaccag gcggcggtag cggcggctga cgaacagaaa
    51301 cggcctccca ggggtcaaat ccgccttcgc cacatatcgc gacgctgtcg ggatcaacgg
    51361 ataacgctgc cgccagctcc ttcgcgctag tcatccccac cgccagtttg cgcccccagc
    51421 tatagcgcgt cgcccacagc cactgttcgt tatcacgcca ggccagacat tgctgatgag
    51481 aaggtaaaaa aggcaggaat cgctgtaatg cactggcatc cggggtgatc gcactaacat
    51541 gaacacgcaa cctttctgcc agagtaagca gcgttgccag ctctttgctt tgcgcggcag
    51601 tcacgttata ggcgggactg agtgagtctt cgctgtaatc gaaacgcagg gagtccggat
    51661 ccatatccag ctcgcgggcc atcgtccctg acagccaggc cgtttgctcc cgctcaccaa
    51721 gggacatcga cgggcgcgga aatgaccgct gtaatgtgcg actggcggga aacgccaaca
    51781 taatgtgatg acgctgcggc agttcgcgac tccacggtaa caacgtttta gccagctgct
    51841 gcgcatcaac aatccgccca tctttgataa tgtcgttctc cagcggcaac cgccaccagc
    51901 gttgcaaaaa gcattctttt gctccccgca cgatcgcaac cgctaccgct tcttgctgtt
    51961 gtaaatgcaa accaatttgc cagatcttaa atgccattgt gatgctctcc ttatcacccg
    52021 tcactctgac gggtatatca atgcgtctgg cttgccttta tactaccgcg cgtttgttta
    52081 taaactgccc aaatgaaact aaatgggaaa tttccagtga agttcgtaaa gtattttttg
    52141 atccttgcag tctgttgcat tctgctggga gcaggctcga tttatggcct ataccgctac
    52201 atcgagccac aactgccgga tgtggcgaca ttaaaagatg ttcgcctgca aattccgatg
    52261 cagatttaca gcgccgatgg cgagctgatt gctcaatacg gtgagaaacg tcgtattccg
    52321 gttacgttgg atcaaatccc accggagatg gtgaaagcct ttatcgcgac ggaagacagc
    52381 cgcttctacg agcatcacgg cgttgacccg gtggggatct tccgtgcggc aagtgtggcg
    52441 ctgttctccg gccacgcgtc acaaggggcg agtaccatta cccagcagct ggcgagaaat
    52501 ttcttcctca gtccagaacg cacgctgatg cgtaagatta aggaagtctt cctcgcgatt
    52561 cgcattgaac agttgctgac gaaagacgag atcctcgagc tttatctgaa caagatttac
    52621 ctcggttacc gcgcctatgg tgtcggtgct gcggcacaag tctatttcgg taaaacggtc
    52681 gaccaactga cgctgaacga aatggctgtg atcgccgggc tgccgaaagc gccttccacc
    52741 tttaatccgc tctactcaat ggatcgtgcc gtcgcgcggc gtaacgtcgt actgtcgcgg
    52801 atgctggatg aagggtatat cacccaacaa cagttcgatc agacacgcac tgaggcgatt
    52861 aacgctaact atcacgcgcc ggagattgct ttctctgcgc cgtacctgag cgaaatggtg
    52921 cgccaggaga tgtataaccg ttatggcgaa agtgcctatg aagacggtta tcgcatttac
    52981 accaccatca cccgcaaagt gcagcaggcc gcacagcagg cggtacgtaa taacgtgctg
    53041 gactacgaca tgcgccacgg ctatcgcggc ccggcaaatg tgctgtggaa agtgggcgag
    53101 tcggcgtggg ataacaacaa gattaccgat acgctgaagg cgctgccaac ctatggtccg
    53161 ctgctgcctg ccgcagtcac cagcgccaat cctcagcaag cgacggcgat gctggcggac
    53221 gggtcgaccg tcgcattgag tatggaaggc gttcgctggg cgcgtcctta ccgttcggat
    53281 actcagcaag gaccgacgcc gcgtaaagtg accgatgttc tgcaaacggg tcagcaaatc
    53341 tgggttcgtc aggttggcga tgcatggtgg ctggcacaag tgccggaagt gaactcggcg
    53401 ctggtgtcga tcaatccgca aaacggtgcc gttatggcgc tggtcggtgg ctttgatttc
    53461 aatcagagca agtttaaccg cgccacccag gcactgcgtc aggtgggttc caacatcaaa
    53521 ccgttcctct acaccgcggc gatggataaa ggtctgacgc tggcaagtat gttgaacgat
    53581 gtgccaattt ctcgctggga tgcaggtgcc ggttctgact ggcagccgaa gaactcacca
    53641 ccgcagtatg ctggtccaat tcgcttacgt caggggctgg gtcagtcgaa aaacgtggtg
    53701 atggtacgcg caatgcgggc gatgggcgtc gactacgctg cagaatatct gcaacgcttc
    53761 ggcttcccgg cgcaaaacat tgtccacacc gaatcgctgg cgctgggttc agcgtccttc
    53821 accccaatgc aggtggcgcg cggctacgcg gtcatggcga acggtggctt cctggtggac
    53881 ccgtggttta tcagcaaaat tgaaaacgat cagggcggcg tgattttcga agcgaaaccg
    53941 aaagtagcct gcccggaatg cgatattccg gtgatttacg gtgatacgca gaaatcgaac
    54001 gtgctggaaa ataacgatgt tgaagatgtc gctatctccc gcgagcagca gaatgtttct
    54061 gtaccaatgc cgcagctgga gcaggcaaat caggcgttag tggcgaagac tggcgcgcag
    54121 gagtacgcac cgcacgtcat caacactccg ctggcattcc tgattaagag tgctttgaac
    54181 accaatatct ttggtgaacc aggctggcag ggtaccggct ggcgtgcagg tcgtgatttg
    54241 cagcgtcgcg atatcggcgg gaaaaccggt accactaaca gttcgaaaga tgcgtggttc
    54301 tcgggttacg gtccgggcgt tgtgacctcg gtctggattg gctttgatga tcaccgtcgt
    54361 aatctcggtc atacaacggc ttccggagcg attaaagatc agatctcagg ttacgaaggc
    54421 ggtgccaaga gtgcccagcc tgcatgggac gcttatatga aagccgttct tgaaggtgtg
    54481 ccggagcagc cgctgacgcc gcctccgggt attgtgacgg tgaatatcga tcgcagcacc
    54541 gggcagttag ctaatggtgg caacagccgc gaagagtatt tcatcgaagg tacgcagccg
    54601 acacaacagg cggtgcacga ggtgggaacg accattatcg ataatggtga ggcacaggaa
    54661 ttgttctgat taaaaaggcg cttcggcgcc ttttcagttg gctgacaaag tgcatttgtt
    54721 tatgccggat gcgggtgaac gcgttatccg gctaacaaaa tcgtgaaaaa atcaatatat
    54781 tgcaggaaac ccataagcct gataagcgtt gtgcatcagg caaacttcac gcatttacac
    54841 tcgcccctgc cctttcaacc attcgcgcac gaggaacagc gcgctgacat tacgcgcttc
    54901 gttgaagtca gggtcttcca gcaaatccat catatgcgcc agcggccagc gcacctgtgg
    54961 tagcggctct ggctcatcgc cttccagcga ttccgggtag agatcttgcg cgaccacgat
    55021 attcattttg ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa aagtcagatc
    55081 gttcgcacca aatccaacct cttcttttag ctcgcggtta gcggcttcgt agacgctttc
    55141 acccggatca attaatcctt tcgaaaaacc taattcgtag gattcagttc ccactgcgta
    55201 ttcgcggatc aggatcaggt gatcgtccac aatcggcaca atcatcactg cttcccggtt
    55261 ggttggacgc attcgttcat aaacacgccg cacgccattg ctgaactcca gatccacgct
    55321 ctcgacggta aacagtcggg aacgggctac agtttcaaca ttcagaatgg tgggtttttg
    55381 taatgatttg ctcatcgtgg gatctatgct gtgaaatcag cggttattgt gcgatatcgg
    55441 acacgctttc ggcaatgtga attgcatgtt atttacattt atgtaactta ataaataatc
    55501 gtcctcaaat caaattaaaa gtcaataggt tgaaataact ccaggaattt gctgatattc
    55561 cgccttcaga gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt
    55621 taaactccac gcttgccgat agccaaccgc agaatcatgt attgtgtctg gtgcgactga
    55681 ccacgcctga cagactaagt aagatgggga aagcatgagc accattgtga tttttttagc
    55741 tgctttgctg gcctgctcac tacttgcggg atggctgata aaagtgcgat ccagacggcg
    55801 tcagctgccc tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga
    55861 acgtagcgcc gttgaaaatt atcttgagag cctgacgcag gtattacagg tgcctggccc
    55921 aacgggagcc agcgcggcac cgatctctct ggcgctgaat gccgaaagca acaacgtcat
    55981 gatgctgaca cacgctatca cgcgttacgg catctctacc gacgatccga ataaatggcg
    56041 ttactacctc gattcggtag aagtccacct gccccctttc tgggaacaat acatcaacga
    56101 tgagaatacc gttgaactga tttataccga ttcgctgccg ctggttattt cactcaacgg
    56161 tcatacgctg caggagtaca tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc
    56221 cacgcaggcg tcgattcgcg gggaagagag tgagcaaatc gagctactca atattcgcaa
    56281 agaaacgcat gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat
    56341 cgtcgcctcc ttcctgatgt tctttttctg cctgattacc ccggatgtat ttgttccgtg
    56401 gctggcaggc ggcgcattac tgctgctggg cgcaggtctg tgggggctat tcgcgccccc
    56461 ggcaaaatcc tccctgcgag aaattcattg tctgcgcggt acaccccgtc gttggggatt
    56521 gtttggcgaa aacgatcagg aacagatcaa caatatttca ctcggtatta tcgacctggt
    56581 ctatcccgca cactggcagc catacattgc tcaggatctc ggtcaacaaa ccgatattga
    56641 tatctatctc gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt
    56701 aaaaaacttt ccgttacagc actggctgcg cagtacgatt atcgctgcgg gctcgctgct
    56761 ggtgctgttt atgctgttat tctgggtccc gctggatatg ccgctgaaat tcactctctc
    56821 atggatgaaa ggcgcgcaga ccattgaagc caccagcgta aaacaactgg ctgacgctgg
    56881 cgtgcgggta ggcgatacat tgcgtattag cggtacggga atgtgtaata ttcgcacttc
    56941 cggaacctgg agcgcgaaaa ccaattcacc ttttttaccg tttgactgct cgcagatcat
    57001 ctggaatgac gcccgctcat taccattacc agaatctgaa ctggtcaaca aagcgacggc
    57061 attgactgaa gcagttaatc gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag
    57121 tgcctcatta cgttcagcga ttcaaaaatc cggcatggta ttgcttgatg attttggcga
    57181 cattgtactg aagacagcgg atttatgttc tgccaaagat gactgtgtgc gactgaaaaa
    57241 tgcgctggtc aatctcggca acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc
    57301 cgggaagctc gatggcgtga atgtgttatt acgcccggtg agtgcagaat cgctggataa
    57361 cctggtggca acctccaccg cgccgtttat cacgcatgaa acggcgcgag cggcacaatc
    57421 actaaacagt ccggcccccg gcggattcct gattgtcagc gacgaaggca gcgattttgt
    57481 tgatcagccc tggccttcgg catcacttta cgactacccg ccgcaagaac agtggaacgc
    57541 tttccagaaa ctggcacaaa tgctgatgca tacgccgttt aacgccgaag gtatcgtcac
    57601 aaaaatcttc actgacgcca atggtacgca gcatattggc cttcatccga tcccggatcg
    57661 ttccggactg tggcgctatc tcagcaccac attgctgcta ctgacgatgc tgggtagcgc
    57721 catttacaat ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc gcatgatgga
    57781 gattcaggcc tattatgaaa gctgcctgaa cccgcaactg atcacccctt cagaaagcct
    57841 tatcgaataa cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc
    57901 tggagatttc cctcatgcat atcaacattg cctggcagga cgtagatacc gttctgctgg
    57961 atatggacgg cacgttgctc gacctcgcct tcgataacta tttctggcaa aagctggtgc
    58021 ctgaaacatg gggcgccaaa aacggggtta cgccacagga agcgatggaa tatatgcgcc
    58081 agcaatatca cgacgtacag catacgctaa actggtactg tcttgattac tggagtgagc
    58141 aactgggtct ggatatctgt gcgatgacca ccgagatggg accgcgtgcc gtactgcgtg
    58201 aagataccat tccgtttctt gaggcactga aagccagcgg taagcagcga attttgctca
    58261 ccaatgcgca tccgcacaac ctggcggtaa aacttgagca taccggtctg gacgcacacc
    58321 ttgatttatt actttccacc cacacatttg gttatccgaa agaggatcag cggttatggc
    58381 atgcggtggc cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg
    58441 aagcgattct cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc
    58501 ctgattccgg gattgccgag aaacagtatc aacgccatcc gtcactgaat gactaccgcc
    58561 gcctgatccc ctcgctaatg tgaaggagac gccatgaaag agaaacctgc tgttgaggtt
    58621 cgactggata aatggctatg ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa
    58681 atgattgaag gcggtaaggt gcattacaac gggcagcgca gcaagccgag caaaatcgtc
    58741 gagctgaatg ccacgctcac tctgcgccag ggaaatgacg aacgcacggt gattgtaaag
    58801 gcgattactg aacagcgtcg ccccgccagc gaggcagcct tgctgtatga agagactgcg
    58861 gaaagtgtag agaaacgcga aaaaatggcg atggcacgta aacttaatgc cttaaccatg
    58921 ccgcacccgg accgacgccc ggacaaaaaa gagcgccgcg acctgttacg atttaaacac
    58981 ggcgacagtg aataactgtc acctgcaaga gagatgatta tgccgcaaca tgaccaatta
    59041 catcgctatc tgtttgaaaa ttttgccgtt cgcggcgaac tggtaaccgt ttcggaaacc
    59101 ctgcaacaga tccttgagaa ccacgattat ccgcagcccg ttaaaaacgt gctggcagaa
    59161 ctgctggttg cgaccagcct gttaaccgct acgctgaagt ttgatggtga tatcaccgta
    59221 cagctgcaag gcgacggtcc gatgaatctg gcggttatta acggtaacaa taaccagcaa
    59281 atgcgcggtg tggcgcgcgt gcagggcgaa attccagaaa atgccgacct gaaaacgctg
    59341 gtcggcaatg gttacgtggt gatcaccatt accccgagcg aaggcgaacg ctatcagggc
    59401 gtggttggtc tggaaggtga taccctggcg gcctgcctgg aagattactt tatgcgttct
    59461 gaacagctgc cgacgcgcct gtttattcgc accggcgacg tagacggcaa accggctgca
    59521 ggcggtatgt tgttgcaggt aatgcctgcg caaaatgccc agcaggacga ctttgaccac
    59581 ctggcgacgc taaccgaaac catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa
    59641 gtgttgtggc gtttgtatca cgaagaagag gtgacggttt acgatccgca ggatgtggag
    59701 ttcaaatgca cctgctcgcg tgaacgttgc gccgatgcgc tgaaaacgct gcctgatgaa
    59761 gaagttgata gcatcctgac ggaagatggc gaaattgaca tgcattgtga ttactgcggt
    59821 aaccactatc tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc gtctccggca
    59881 gatccgcaag ttcattaatg agtatgtccg gcagagaggg ctctgccgga ttttacgacg
    59941 taatggcctg atgtgctgcg cttaccaggc ctgtgaaata aatcgcaaat tattgttggt
    60001 cagatacagc gtcgtatccg ttaaaagagt tgtcacttac ttaagtttat ttccagcgtt
    60061 tcaccatcta ccgtcagatc gcgccacgct ttcggcctcg tccccagttt tccatccttc
    60121 gcagctgtga gtaatttttc tttcgttatt tcgttcggaa gtaaactcat atcaagcgcg
    60181 tcccattctt ttctgtctga gtcaaagcca tagacaatga ctctgtcatc attaaaagca
    60241 aacaggatcc gctccgcctg accatcacta ttcagatctt gctcgaccag gacgcaggcg
    60301 tctttttcaa tacaggtcat cacgttatag cgatcctgga ttaaggccga ccagaatgtc
    60361 gcatccggtt taacagaacc aggggcaatt aacacatttt cggctaatac tttttccgat
    60421 acctgttcct gaagatgttg ctctccatct aacgccatca gcagatcccg cgcgcgtttc
    60481 gggtctttca taaacccggc atcgcttttc agcgactcaa gcgccgcacg tccatagcga
    60541 ccgctctgtt cgagcatgta gatagttacc tggtctggcg tgtttttgcc gctctgataa
    60601 cgcgccatat gactgttcac gctaatacgc atactatcca gcaccggcga attaagaagc
    60661 accagtatca ccaacaccaa taacgaaacc gcaaggttca ctttgccctg aagaacaagg
    60721 ggattttgcc cattacgcca gacgatactg acaaaatacc ccagcgacca caccagtaac
    60781 accagcaccg ccagcgcgcc ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc
    60841 agccataacg cccaggcggc gacgaacaca tacagcggag caaccagcaa agcggtttta
    60901 atcaggcaac gcaacggccc cgtccaggga agtgacgctt tttgcggatc gcggacaata
    60961 gccattaaga tcaattgcaa aaaggccagc gttaacagca acccggcggc ggagatgtgg
    61021 cgagaaatcg cgctcaggcc cgtaaacggc agagtgatga taaacatcag ggttagcaat
    61081 gatactaacg gcagcaaccc cgtggcgatt aacgtgaaca acttttgaat agagtcgatt
    61141 aaacgtgact gtgtgcgcgc gagaatcacc gccagtgcgg taaccagacc taatgtaaga
    61201 tacataaacc agtcggttgc aaaaaagagc gtattgaaaa acttgatgcc aacaagttta
    61261 aacaactcac cccacagtaa aagcaccacc cacgtcaggc cattggcgag aaaaatcacc
    61321 agtaatataa gtacgttatg ccatactgac ttgtaaaagt agctatagcg gctactgcca
    61381 ttgcggatac gaaggctttg ttgtatccac ggtagcaaca acattgccat caacagcaga
    61441 tagcagccaa aatcccaaat cgccttttca gctctccagg ggttcatgcc atcagtttgc
    61501 cacttcagcc aaccgctcat ccctgtcgtg gcaataaaca ccagcgccag ccatccccaa
    61561 aggcgcttct gtttaaaaga gatcacggag aagagtaaaa cggatgaaaa cgcaacggtc
    61621 gcaggcacgc cataaacaat ccaactgtga ttttttccgg caagccaggc gatcaacagg
    61681 tagcaaacca gcccctgaag taatccggta gcaatcatcc cccagcgtgt tgccggtgaa
    61741 agttcaacgt tatccatgtt ggttatccag aatcaaaagg tgggttaatt atcgcatccg
    61801 ggcagtagta ttttgctttt ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat
    61861 actttaccga ttgaatttgc atcaatttca ttaaggaatg cgattccact cacaatattc
    61921 ccgccatata aaccaagatt taaccttttg agaacatttt ccacacctaa aatgctattt
    61981 ctgcgataat agcaaccgtt tcgtgacagg aatcacggag ttttttgtca aatatgaatt
    62041 tctccagata cgtaaatcta tgagccttgt cgcggttaac acccccaaaa agactttact
    62101 attcaggcaa tacatattgg ctaaggagca gtgaaatgcg cgttaacaat ggtttgaccc
    62161 cgcaagaact cgaggcttat ggtatcagtg acgtacatga tatcgtttac aacccaagct
    62221 acgacctgct gtatcaggaa gagctcgatc cgagcctgac aggttatgag cgcggggtgt
    62281 taactaatct gggtgccgtt gccgtcgata ccgggatctt caccggtcgt tcaccaaaag
    62341 ataagtatat cgtccgtgac gataccactc gcgatacttt ctggtgggca gacaaaggca
    62401 aaggtaagaa cgacaacaaa cccctctctc cggaaacctg gcagcatctg aaaggcctgg
    62461 tgaccaggca gctttccggc aaacgtctgt tcgttgtcga cgctttctgt ggtgcgaatc
    62521 cggatactcg tctttccgtc cgtttcatca ccgaagtggc ctggcaggcg cattttgtca
    62581 aaaacatgtt tattcgcccg agcgatgaag agctggcagg tttcaaacca gactttatcg
    62641 ttatgaacgg cgcgaagtgc actaacccgc agtggaaaga actgggtctg aactccgaaa
    62701 acttcgtggc gtttaacctg accgagcgca tgcagctgat tggcggcacc tggtacggcg
    62761 gcgaaatgaa gaaagggatg ttctcgatga tgaactacct gctgccgctg aaaggtatcg
    62821 cttctatgca ctgctccgcc aacgttggtg agaaaggcga tgttgcggtg ttcttcggcc
    62881 tttccggcac cggtaaaacc accctttcca ccgacccgaa acgtcgcctg attggtgatg
    62941 acgagcacgg ctgggacgat gacggcgtgt ttaacttcga aggcggctgc tacgcaaaaa
    63001 ctatcaagct gtcgaaagaa gcggaacctg aaatctacaa cgctatccgt cgcgatgcgc
    63061 tgctggaaaa cgtcaccgtg cgtgaagatg gcactatcga ctttgatgat ggttcaaaaa
    63121 ccgagaacac ccgcgtttct tatccgatct atcacatcga taacattgtt aagccggttt
    63181 ccaaagcggg ccacgcgact aaggttatct tcctgactgc tgatgctttc ggcgtgttgc
    63241 cgccggtttc tcgcctgact gccgatcaaa cccagtatca cttcctctct ggcttcaccg
    63301 ccaaactggc cggtactgag cgtggcatca ccgaaccgac gccaaccttc tccgcttgct
    63361 tcggcgcggc gttcctgtcg ctgcacccga ctcagtacgc agaagtgctg gtgaaacgta
    63421 tgcaggcggc gggcgcgcag gcttatctgg ttaacactgg ctggaacggc actggcaaac
    63481 gtatctcgat taaagatacc cgcgccatta tcgacgccat cctcaacggt tcgctggata
    63541 atgcagaaac cttcactctg ccgatgttta acctggcgat cccaaccgaa ctgccgggcg
    63601 tagacacgaa gattctcgat ccgcgtaaca cctacgcttc tccggaacag tggcaggaaa
    63661 aagccgaaac cctggcgaaa ctgtttatcg acaacttcga taaatacacc gacacccctg
    63721 cgggtgccgc gctggtagcg gctggtccga aactgtaatg atttgaagct ggagaatatc
    63781 tatccagtat cttatagaaa gcaaaacggg aggcaccttc gcctcccgtt tatttaccct
    63841 tcttttgtcg tgccctgcgc ccgcgttacc ggcactggca gccaggcgcg aatggaaagc
    63901 ccgccccgct cgctggtgcc aagctccagc atcccgttat ggttatccac gatacgctgc
    63961 acaattgcca gccctaatcc cgtgccgcta atggtgcgcg cactgtcgcc gcggacaaac
    64021 ggctggaaca ggtgcttacg ttgttccggc gcaattcccg gaccgtcatc ttccacctgg
    64081 aaccaggcgc gattcggctc cgttccgctg ctgactttga tccagccatt gccataacgg
    64141 gcggcgttga ccaccatatt cgccaccgcg cgtttgatcg acagcgggtg cattttcacg
    64201 tcaatgctgc cggggtaaag cgcggtttca atttcccgct catagccact ttcggcagca
    64261 atcacctcac cgagtactgc attaagatcc gccatttcca tcggcatctc ctgcccggtg
    64321 cgcaggtagt cgataaactg ctcaatgatg gcgttgcact cttcgatatc tttattgatc
    64381 gattctgcca gatagccatc ctgctcgctc atcatctcag tcgccaggcg aatacgcgtc
    64441 agcggcgtgc gcaagtcgtg acttaccccc gccatcagca gcgtgcggtc atccgccagt
    64501 tgcttaacac cagccgccat atggttaaag gcacgggtaa cggaacgcac ctcggacgcg
    64561 ccatactcac gcagcggcgg cggaataatc cctttaccaa cctgcaaggc tgcgtgttcg
    64621 agatcgacca acggtcggtt ctggatacga ataaacagcc acgccccgcc tatcgccaat
    64681 agcataatcg ccagcgtata gcggaacagc ggagagaaat cgccctgatg aatttcggtc
    64741 agcggcacgc gtacccagat attgggcgac agccaggttt tcagccagac gacaggcgaa
    64801 cttttgttga cctcaacgcg cacttccgtc gggccgccca gttgctgtgc catctgatgg
    64861 cttaagaatt catagtgttg cgcccaacgc agacctgcct cttcggcagc ctcgttggag
    64921 tagagagaga tccccagctc acggtagatc tcccgacgga aagcgggagg cacaaccaac
    64981 tgcgtgccgt cctccagttg cagtttgtcg gtcatcaaca tacgcacttc gtacgcgagg
    65041 actttattaa actgctggag gctcggcaaa atcgcgaagt tcagcaccac cagataagtc
    65101 gtcaccaggc tggcgaacag caaggtgacg atgagcaata acgtacgggc aaatgaactt
    65161 cgtggcgaga agcgcaatcg cctcatgctt tagagccgtc cggtacaaag acgtagccca
    65221 ggccccaaac ggtctgaatg taacgcggat gcgctggatc ttcttctacc atgcggcgca
    65281 gacgcgaaat ctgcacgtcg atggagcgtt ccattgcgga atattcacga ccacgggcaa
    65341 ggttcatcag cttatcgcgg gagagcggct cacgcggatg gctgaccagt gccttcagta
    65401 ccgcaaactc accgctggtg agcggcatcg gctcatcttc gcggaacatt tcgcgcgtac
    65461 cgaggttaag tttgaactta ccgaaagcaa ttaccgcctc ttcctgtgac ggtgcgcctg
    65521 gcagttcgtt cgcctgacga cgcagcaccg cacgaatacg ggccagcagt tcacgcgggt
    65581 taaacggttt tggaatgtag tcgtcagcgc caatctccag gcctacgata cggtccactt
    65641 cttccccttt cgccgtcacc ataatgatcg gcatcgggtt gctctgacta cgaagacgtc
    65701 ggcaaatcga caagccatct tcaccaggta acattaaatc cagtaccata agatggaaag
    65761 attcacgagt cagcaggcga tccatctgtt ctgcattagc gacgcttcga acctggaagc
    65821 cttgttcggt gagataacgt tccagcagcg cacgcaggcg catgtcgtca tcgaccacca
    65881 gaatcttgta gttctcttgc attgtttgta ctcccaaagg ttcgcaacaa tttgtaagcg
    65941 tgtattctta aaaaagctca cgttcgtcac cagctaaatc tggtatgaat ttcagcctaa
    66001 attgttacaa agcatattaa acagcagctt aagtatacaa tttattcggc gaaacattat
    66061 tgattctgtt gatatgatca cgttataccc aatgtgcgca ttatcaaaca gacaaaggga
    66121 atcaacgaga tgaaaacgcc cctggttacc cgggaagggt atgaaaaact caaacaagag
    66181 cttaattatc tctggcgtga agaacgcccg gaggtcacaa aaaaggtgac ctgggccgca
    66241 agtctgggcg accgcagcga aaatgctgac tatcagtata ataaaaagcg tctgcgtgaa
    66301 atcgaccgtc gcgtgcgcta tctcactaaa tgcatggaaa atctcaaaat cgtcgattac
    66361 tcccctcagc aggaaggcaa ggtctttttt ggcgcatggg tggagattga aaacgacgat
    66421 ggcgtgactc accgtttccg tattgtcggc tacgatgaaa tttttggccg taaagattac
    66481 atctctatcg attccccgat ggcccgcgca ttgctgaaaa aagaagtcgg cgatctggcg
    66541 gtggtgaata cccctgccgg ggaagcgagc tggtatgtca atgctatcga gtacgtgaaa
    66601 ccgtaaggaa gagtcttaac ctcctgccga tggctggcat ttttgccagc cagtccgtat
    66661 aactatcccc tgatttttga tccgaaaaga tgaactcaaa ccatgatgaa tgattcgttc
    66721 tgccgcatta ttgcgggtga aattcaggcg cgcccggaac aggttgacgc tgccgttcgc
    66781 ctgcttgacg aagggaatac cgtgccgttt atcgcacgtt atcgtaagga aatcaccggc
    66841 ggtctggatg acacgcagct gcgtaatctg gaaacgcgtc tgagctatct gcgcgagctg
    66901 gaagagagac gtcaggcgat cctcaaatcc atttccgagc aaggcaaact caccgatgat
    66961 ctggcgaagg ccatcaacgc cacactaagc aaaaccgaac tcgaagatct ctacctgccc
    67021 tacaaaccta aacgccgcac ccgcgggcaa atcgccattg aagcagggct tgagccgttg
    67081 gctgacctgc tgtggagcga tccgtcacac acgccagaag tcgccgctgc acaatatgtt
    67141 gatgcagata aaggcgtggc agataccaaa gccgcgctgg acggcgcgcg ctatatcctg
    67201 atggaacggt ttgccgaaga tgccgcgctg ctggcgaaag tgcgtgatta tctgtggaag
    67261 aacgcgcatc tggtctcaac cgtgatgagc ggtaaagaag aggaaggggc aaaattccgc
    67321 gactatttcg atcatcacga accgctgtcc acggtgcctt ctcaccgcgc gctggcgatg
    67381 ttccgtgggc gtaacgaagg cgtactccag ctttcgctga atgccgatcc gcagttcgaa
    67441 gagccgccaa aagagagcta ttgcgagcaa atcatcatgg atcatctcgg cctgcgcctg
    67501 aacaatgctc cggcggatag ctggcgcaaa ggcgtggtga gctggacctg gcgcatcaag
    67561 gtgctgatgc atctggaaac cgaactgatg ggcaccgtgc gcgaacgcgc ggaagatgaa
    67621 gcaatcaacg tctttgcccg taacctgcac gatctgctga tggcggcccc tgccggactg
    67681 cgtgccacga tgggcctcga tccgggtctg cgtaccgggg taaaagtagc ggtggtcgat
    67741 gccactggca aactggtggc gaccgatacc atttacccgc acaccggaca aacagcaaaa
    67801 gcagcgatga ccgttgctgc cttgtgtgaa aagcataacg ttgaactggt ggcgatcggc
    67861 aacggtacag cttcccgcga aaccgaacgt ttctatctcg acgtgcagaa gcagttcccg
    67921 aaagtgaccg cgcagaaagt gatcgtcagc gaagctggcg cgtcggttta ctcggcttcc
    67981 gagctggcag cgcaggagtt cccggatctc gacgtttcgc tgcgtggtgc ggtatctatc
    68041 gcccgccgtt tgcaggatcc gctggcggag ctggtgaaaa tcgatccgaa atctatcggc
    68101 gtaggtcagt atcagcatga cgtcagccag acgcaactgg cccgcaaact ggatgcagta
    68161 gtagaagact gcgtaaacgc cgttggcgtc gatctcaaca ccgcttctgt tccgctatta
    68221 acccgcgtgg cgggcctgac gcgcatgatg gcgcaaaaca tcgtggcctg gcgcgatgag
    68281 aacggccagt tcaagaaccg tcagcaactg ctgaaagtca gccgtctggg gccgaaagcc
    68341 ttcgagcagt gcgcgggctt cctgcgcatt aaccacggtg ataacccgct ggacgcctct
    68401 accgttcacc cggaagccta tccggtggtg gaacgcattc tggcagcaac acagcaggca
    68461 ctgaaagatc tgatgggtaa cagcagcgaa ctgcgtaacc tgaaagcgtc tgactttact
    68521 gatgaaaaat tcggtgtgcc gacagtaact gacatcatca aagagctgga aaaaccgggt
    68581 cgcgatccgc gtccggaatt taaaaccgct cagtttgccg atggcgtcga gacaatgaac
    68641 gacctgcaac cgggtatgat cctcgaaggc gctgtcacca acgtcaccaa ctttggcgcg
    68701 tttgtcgata ttggcgtgca tcaggacggc ctggttcaca tctcttcatt gtcgaacaag
    68761 tttgtggaag atccgcatac cgtggtgaaa gcgggcgaca ttgtgaaggt gaaagtgctg
    68821 gaagtggatc ttcagcgtaa acgtatcgcc ctgaccatgc gtctggacga gcagcctggc
    68881 gaaaccaacg cccgtcgcgg cggcggtaat gaacgcccgc aaaacaaccg cccggcagcc
    68941 aaaccacgcg gtcgtgaagc gcagcctgcc ggtaatagcg cgatgatgga tgcgctggcg
    69001 gcggcaatgg gcaaaaaacg ttaaacgccc tgattgaccc ctaaacctgc gcgataaggt
    69061 gtccacgccg catctggcaa cgaacgcctt gtctgacata aaagtgccgg agaatatctc
    69121 cggcattttt attccacagc caaactcaca atatattccg gcaacattta tcacttcatt
    69181 aacagctgaa accttaatta aacattagcc agtcctggta attcgctatt cgaattatat
    69241 tttcgctgcg atataacctt gagccacatc aacattgagt cagattatta ttcaaaccaa
    69301 cattcgcaca cattttaagt attgctgata gaaaccattc tcattatcat tgtgctgttg
    69361 attatttaat ctctccttcg ttggcaaatc atctggtctc atgtcgctgt caaacgcccc
    69421 atgaggtagt tatccagtta atgagaaaca agtaggcacc tatgcaatac actccagata
    69481 ctgcgtggaa aatcactggc ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc
    69541 tttctcttgg catgttacct ggctcctctt ttaatgtggt gcgcgtcgct ccactcggcg
    69601 accccattca tatcgaaacc cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct
    69661 tattagaagt ggaagcggtt tcctgttaat acggtgataa caacaatgaa aaaattaacc
    69721 ataggcttaa ttggtaatcc aaattctggc aagacaacct tatttaacca gctcactggc
    69781 gcacgtcagc gtgtaggtaa ctgggctggc gttaccgtcg aacgtaaaga agggcaattc
    69841 tccaccaccg atcatcaggt cacgctggtg gacctgcccg gcacctattc tctgaccacc
    69901 atctcatcgc agacctcgct cgatgagcaa atcgcctgtc actacatttt gagtggcgac
    69961 gccgacctgc tgattaacgt ggtggatgcg tctaaccttg agcgtaacct gtacctgacg
    70021 ctacaactgc tggaactcgg cattccctgc atcgtggcac tgaacatgct cgacattgcc
    70081 gagaagcaaa atattcgtat tgaaattgat gctctgtcgg cgcgtcttgg ctgcccagtg
    70141 atcccgctgg tttcaacccg tggtcgcggt attgaagcgc tcaagctggc gattgatcgc
    70201 tataaagcta acgagaatgt ggaactggtg cattacgcac agccgctact caacgaagca
    70261 gattcactgg caaaagtgat gccttccgac atcccgctga aacaacgtcg ctggctgggc
    70321 ctgcaaatgc tggaaggcga tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat
    70381 ctggatgccg ccctcgcccg tctgcgtaat gagatggacg atccggcgct gcacattgcc
    70441 gatgcgcgtt accagtgcat tgctgccatc tgtgatgtgg taagcaacac cctgacggca
    70501 gaacccagcc gtttcaccac tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg
    70561 ccgattttcc tctttgtgat gtacctgatg ttcctgctgg ctatcaacat cggcggggcg
    70621 ttacagccgc tgtttgacgt cggctccgtg gcgctatttg tgcatggtat tcaatggatt
    70681 ggctacacgc tccacttccc ggactggctg actatcttcc tcgcccaggg cctgggtggc
    70741 ggcattaaca ccgtgctgcc actggtgccg cagattggca tgatgtacct gttcctctcc
    70801 ttccttgagg actccgggta tatggcgcgt gcggcgtttg tgatggaccg tctgatgcag
    70861 gcgctgggct tgccggggaa atcctttgtg ccgctgatcg tcggtttcgg ttgtaacgta
    70921 ccgtcggtaa tgggtgcacg tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg
    70981 atggcaccgt ttatgtcctg cggcgcgcgt ctggctatct tcgcagtatt cgcggctgcc
    71041 ttcttcgggc agaacggtgc gctggcggtc ttctcgctgt atatgctggg tattgtgatg
    71101 gcggtgctga ctggcctgat gctcaagtac accatcatgc gcggtgaagc gacgccgttt
    71161 gtcatggagt tgccggtcta tcatgtacca cacgttaaaa gcctgattat ccagacctgg
    71221 cagcgtctga aaggcttcgt tctgcgtgct ggtaaagtga tcatcatcgt cagcattttc
    71281 ctgagcgctt tcaacagctt ctcgctgagc gggaaaatcg tcgataacat caacgactcg
    71341 gcgctggcgt ccgtcagccg ggtgatcacc ccggtcttca agccaattgg cgtgcatgaa
    71401 gataactggc aggcaacggt tggcctgttt acaggtgcca tggcgaaaga agtggtagtg
    71461 ggtacgctca acaccctcta caccgcagaa aatattcagg acgaagagtt caatccggca
    71521 gagtttaacc tcggtgaaga gctgttcagt gcgatagatg aaacctggca gagcctgaaa
    71581 gacaccttca gccttagcgt actgatgaac cccattgaag ccagcaaagg cgacggcgaa
    71641 atgggtaccg gggcgatggg cgtgatggat cagaaattcg gtagcgcagc agcagcttac
    71701 agctacctga ttttcgtcct gctgtatgta ccatgtatct cggtgatggg agccatcgcc
    71761 cgtgaatcaa gccgtggctg gatgggcttc tccatcctgt gggggctgaa tatcgcttac
    71821 tcgctggcaa cattgttcta tcaggtcgcc agctacagtc agcacccaac ttacagcctg
    71881 gtgtgcattc tggcggttat cctgtttaac atcgtggtta tcggtctgct gcgccgcgcg
    71941 cgtagccggg tggatatcga actgctggca acccgcaagt cggtaagcag ttgctgcgca
    72001 gccagcacca ccggtgattg ccattaatgg cttcacttat tcaggtgcgc gatttgctgg
    72061 cgttacgggg ccgtatggaa gcggcccaga taagccagac attgaacact ccacagccaa
    72121 tgattaacgc catgctgcaa caactggaaa gtatgggcaa agccgtgcgg attcaggaag
    72181 aacctgacgg ctgcctctct ggcagttgta aaagctgccc ggaaggaaaa gcctgtctgc
    72241 gcgagtggtg ggcgctgcgt taaccttact ccatcgcctg ttttggaaag cccggtatgc
    72301 gtctgcatcc gggctttttt gcgtgcggct ttccataaaa atgcaactct tgcagcacgg
    72361 cgtaagttcc tttgaaagca tctcgcaggg atgaaaactc gctaatacac aggtgtggag
    72421 tggcgcgtag agtcgcggca ttcaaacaac aggtgaagga acgccatgag caaaaagcag
    72481 agttccaccc cacacgatgc gctgttcaaa ctctttttac gccaaccgga cacggctcgt
    72541 gattttcttg cgtttcattt accggcaccc attcacgcgc tttgtgatat gaaaaccctc
    72601 aagctggagt cgagcagctt tattgatgac gatctgcgtg aaagctattc cgatgtgctg
    72661 tggtcggtga aaacggaaca aggaccagga tacatctatt gtctgattga acatcaaagc
    72721 acctcaaaca aactgatcgc atttcgcatg atgcgttacg ctattgccgc aatgcaaaat
    72781 caccttgatg ctggatacaa aacgttgccg atggtggtgc cattgttgtt ttaccacggt
    72841 attgaaagcc cctatcccta ttcgctgtgt tggctggatt gtttcgccga tcccaaactg
    72901 gcaaggcagc tttatgcctc cgcatttccg ctgattgatg tcaccgtcat gcctgatgat
    72961 gaaatcatgc agcaccgacg tatggcgctg ctggagttaa ttcaaaaaca tattcgtcaa
    73021 cgcgatctga tggggctggt agagcaaatg gcctgcttat taagtagtgg atacgctaat
    73081 gacagacaaa tcaaagggct gtttaattac atactgcaaa ccggcgacgc ggtacgtttt
    73141 aacgatttta tcgacggcgt tgccgaacgt tcaccgaaac acaaggagag tttaatgaca
    73201 attgcggaaa gattgcggca ggagggggaa caatccaaag ccctgcatat agccaaaata
    73261 atgcttgaat ccggagtccc tcttacagac atcatgcgct ttaccggggt gtcagaagaa
    73321 gagttggctg cggcgagcca gtaaatttct gtctcgccat ttcaaaagtt gcctacaccc
    73381 tctgcttcaa cgccaccagc aggtgacaaa actcgaccgg atgcgaaata aacggcgcat
    73441 gggccgcttt ggcgaagata tatgattcgc tgtgaggcca aagtttatcc agcatcggca
    73501 ccactttgcg cggcaccaga ccgtcgagat agccatacaa tcgcaaaaac ggcatggaca
    73561 cgttttgcag cggctgacgg agatcgaccg ttttcaggat ttccagcccg ccattaagca
    73621 cgtcaacctc cggcatcggt aacgccagaa cggttttctt caacgcccgc gcatcctggc
    73681 gcgccgtttc agtccccatg gtttgtaacg ccaggaaccg ctccactgta cgctgaaaat
    73741 catcactgag ttgctgctga aatcccgcca gtacgtccgg ttttatcccc ggccactcgt
    73801 cacgagcact aaaacaaggt gacgacgcca cggtgaccag cgcctgaaca cgctcgggat
    73861 gggttaaaac aatttggctt gccaccagcc cgcccagact ccagcctaac caaatggctt
    73921 tatcaggtgc ctgttgcagc acggcttcgg ccatatcagc aagtgacagc gcaccaaatc
    73981 cccggctacg cccgaagccg ggcaggtcaa caaggtgcag cgtaaaatgc gagctaagtt
    74041 cctcgtcaat gcaacgccac acttcggcat tcagtcccca tccgtgcagc agcacaagat
    74101 gaacattccc ctgacctttg gtctgccacc agatgttatt catccgctat tgttctcttt
    74161 tgacttacaa ggatgaacat atgctaacag taccgggatt atgctggcta tgccgaatgc
    74221 cactggcgtt aggtcattgg gggatttgtt cggtctgttc acgcgccacc cgcacagata
    74281 aaacgttatg cccacaatgt ggattacccg ccacacactc ccatcttccc tgcggtcgct
    74341 gcctgcaaaa accgccgccc tggcaaagac tggtcacggt tgccgactat gcgccgccgt
    74401 taagtccgct tatccaccag cttaaatttt cccggcgcag tgaaatcgcc agcgccctgt
    74461 cacgtctgtt actattggaa gtcttacacg ctcgtcgcac caccgggttg caattgccgg
    74521 atcgcatcat cagcgttccg ttatggcagc ggcgtcactg gcgtcgggga tttaatcaga
    74581 gcgatttgct gtgtcagccg ttatcacgct ggttgcattg ccaatgggat agcgaagccg
    74641 tcacacgtac acgggccact gcgacccagc attttctcag tgcccggctg cgcaagcgca
    74701 acctgaaaaa tgcctttcgt cttgaattgc ccgtgcaagg tcgccatatg gtgattgtgg
    74761 atgatgtcgt taccaccgga agtaccgtcg cagagattgc gcagttgctt ttacgcaatg
    74821 gtgcggcgac tgtccaggtc tggtgccttt gtcgaacctt gtagagcctc gatgatgggc
    74881 gtattataac caactaaaat agtcaactat taggccatta ctatgatccg tatttccgat
    74941 gctgcacaag cgcactttgc caaactgctg gcaaatcagg aagaagggac acaaatccgc
    75001 gtatttgtga ttaaccctgg cacgcctaac gctgaatgtg gtgtttctta ttgtccgccg
    75061 gatgccgtgg aagccaccga cactgccctg aaatttgacc tgctgaccgc gtatgttgat
    75121 gagttaagcg caccatacct ggaagatgca gagatcgact ttgttaccga ccagttgggt
    75181 tctcagttaa cgctgaaagc cccgaacgcc aaaatgcgta aagtggcaga cgatgcaccg
    75241 ctgatggagc gcgtggagta tatgctgcag tcgcagatca acccacagct tgctggtcac
    75301 ggtggtcgcg tttcgctgat ggaaatcacc gaagacggtt acgccattct gcaatttggc
    75361 ggcggctgta acggttgttc catggtcgat gtgacgctga aagaagggat cgagaagcag
    75421 ctgctgaacg aattcccgga gctgaaaggt gtgcgcgatc tcaccgaaca ccagcgcggc
    75481 gaacactcct actactaagt tatcccctct tttgtagatt gcccgatgcg acgctaaagc
    75541 gtcatttcgg gcctacgttg aacatgtgcc agacgttggc acagcattgc tttggcaaat
    75601 ccctcccttc gtttccccgc gataatatga ccaacctctc ataatttaaa tttaccccgc
    75661 tctggtgatt ctcaaacgcc agatgttacc cgtatcattc acatgggtac caaacatact
    75721 cctgacatct gactacaata attagtttta gtgggtatca gtcgtggtgc cgcaatatct
    75781 ctgttcccga ttgggataat tagagtttgt cgtcagaaaa ttgacgttac ccataacaaa
    75841 tgaaaggcca ggtaaatcat gccattagtc attgttgcta tcggtgtaat cttgttgttg
    75901 ctcctgatga tccgcttcaa aatgaacggc ttcatcgctc tcgtcctcgt ggcgcttgct
    75961 gttggattaa tgcaaggaat gccgctggat aaagttattg gctccatcaa agccggtgtc
    76021 ggcgggacgc tcggtagcct tgccctgatc atgggttttg gcgcaatgct gggcaaaatg
    76081 ctggcagact gcggtggcgc acaacgtatc gccaccacgc tgattgccaa atttggtaaa
    76141 aaacacatcc agtgggcggt ggtactgacc ggttttaccg ttggttttgc cctgttctat
    76201 gaagtgggct ttgtgctgat gctgccgctg gtgtttacca tcgcggcttc tgcgaatatt
    76261 ccactgttgt atgttggtgt accaatggca gctgcactgt ctgtgaccca cggcttcctg
    76321 ccaccgcatc cgggtccgac tgcgattgcc accattttca atgccgatat gggtaaaacc
    76381 ctgctgtacg gtactattct ggcaatcccg accgtgattc tcgccggtcc ggtttacgct
    76441 cgcgtgttga aaggtatcga taagccaatt ccggaaggtc tctacagcgc gaaaaccttc
    76501 agcgaagaag agatgccgag ctttggcgtc agcgtctgga cctctctggt gccggtagta
    76561 ctgatggcga tgcgtgcgat tgccgaaatg atcctgccga aaggtcacgc tttcctgccg
    76621 gtagcggagt tcctcggtga cccggtaatg gctacgctga ttgccgtgct gattgccatg
    76681 ttcacctttg gtctgaaccg tggtcgttca atggatcaga ttaacgacac gctggtttct
    76741 tccatcaaaa tcattgcgat gatgctgttg atcatcggtg gtggcggtgc gttcaagcag
    76801 gtgctggtag actgcggcgt ggacaaatac attgcttcca tgatgcacga aaccaacatt
    76861 tctccgctgc tgatggcctg gtcgattgct gccgtactgc gtatcgcgct gggttccgca
    76921 accgttgcgg caatcactgc gggtggtatc gcggcaccgc tgattgcaac gacgggtgtt
    76981 agcccggagc tgatggttat tgcggtgggt tccggtagtg tgattttctc tcatgtgaac
    77041 gatccgggct tctggctgtt caaagagtac tttaacctga ctatcggcga gaccatcaaa
    77101 tcctggtcga tgctggaaac cattatctcg gtgtgcggtc tggtaggctg tctgctgctg
    77161 aatatggtga tttgataaca ctgactgccg gatgcatcgc atccggcatc aaccgcactc
    77221 tacttcttct tcgctgcagc tctgcgccgt ctgtccaaat ccttcagcaa cttgttcacg
    77281 ccatcatcgg caaacatcga ctcaagcgtt gcggaaagct tgcgtcgcca gtttttatac
    77341 tggtaactgg tgccaggaat attcaccggt tcggccatat ccagccagtc ttccggctgt
    77401 agtcctaaca gagcactgtt actgtcggca atgtagcgct gcaaaccacg gttcagcgtc
    77461 ggcgtcatcg acatcaacga tgccttatgc ccggcacgtt ttggcagaca accatattta
    77521 tgcagtgcat ccagcagccc ttgcttcgcc agttcgcgat cctgatacag accgcgcagt
    77581 accacttcat ccggatacag ccccagggtt ttgcccagcg ttagatcccc gctctcccag
    77641 taaccgcgta gcgtaggcag gtcatgtgtc gccgcaaccg ccatcgactg ctccggatac
    77701 gctttcggtg cacggaacgt cttctcgtgg tcgttttcga aatagagcac tttgtaggag
    77761 tacacaccgc tgctgcgcag cttaccgaca atctctaccg gtacggtacc gagatcttca
    77821 ccaatcacca tacagcgatg acgtttactt tcgagtgcca gaatcgagag cagatcatcc
    77881 accggatagt gaacatacgc gccctgatct gccgtctcgc catacggtat ccaccacaaa
    77941 cgcagcatcg acatcacatg gtcaattcgt aatgcgccgc agttttgcat attggcacgc
    78001 aacagctcga taaacggttc ataggcacgc gcggtgatga tatgcgggtc cattggcggt
    78061 aatccccagt tctgccccaa cgggccgagg atatccggcg gcgcgccaac cgacgctttc
    78121 aggcaatata gttcacggtc acaccaggtt tccgccccac cttccgctac gccaaccgcc
    78181 agatcacgat acaagccaat cggcatttca tagccctggc ttatctccca gcaggcggca
    78241 aactggctgt aagccagcca ctgcaaccag agataaaaat cgacgtcatc acgatgttct
    78301 tcgcagaact gacgcacttc tggtgaatcc acgttctgat acatctctgg ccatgcaggc
    78361 cagccccagc gcatttcgtc ctctttcact tgctgggcat gtagcgcatc aaaggctgcc
    78421 tgccagaaca ggctgtcgcc ctgctctgca acaaactggc gaaacgcggt catctgctca
    78481 tcatcacgtt gcgcgaaacc tttccacgcc attcgtaatg ctgtcatttt tagggcggta
    78541 accgtggagt aatcgaccca gtcggcatcg cgcgcctgtt gcagcgtctg ttgcgtggtc
    78601 ggcaactgcc accaggcctg agcctcttcg ctaagatgga aatcttcaac ggcgttaacg
    78661 tcgatataaa tcacattcag ccaacggcga gaagacgggc tgtatgggct ggcactctcc
    78721 ggatttgccg gatagagcgc atgaatcggg ttcaggccga tgaacgaccc gccacgtttt
    78781 gccacatcca ccagcatcgc tttgagatcg ccaaaatccc caatacccca gtttttttcc
    78841 gatcgcagcg tataaagctg aacgcaggca ccccacagct tttgtttatt cagcaacgcc
    78901 tgcggttcgt aacagcgttt cggggcgaca atcacccggc aatgcgcgcg ctggtcgtcc
    78961 tgggtgagtg tcagcgtgtg ataaccttcc ggcagcttcg tcggtagatt gaacgctttg
    79021 ccccccgtta catggccttt gtactgtgtt ccttcttcgg tggtcagcag ccagctatat
    79081 tcgccgctgc cctccaccac catcggcatt tttttgccgc tggtataaac catgacattc
    79141 gggactggcg ttaccgccac tttcgtggcg gtacgttgat gcatcgcgtc aagcaaacgc
    79201 cgtttggttt cggcgctaat cgactgcggt ttaccgtggg cattgatgta attggggcta
    79261 atccccgccg ccagcgcggc attatccaga cgtttgcttt ccatcgagct tccttagcgt
    79321 tttgcctgcc agatacgagc ctgataatcg cgaatagagc gatccgagct aaacataccg
    79381 cagcgggcgg tattgaggat cgccgcgcga gtccaggcct cctggtcgcg gtacagcaca
    79441 tccacctgct tttgtgcctc aacataggct gcgaaatccg ccatcaccag atacggatcg
    79501 ccgccttgtt tgccgatact gtgcagcatc tggtcgaagg catgcttatc gccgtcgctg
    79561 tatttaccgc tttccagctc tttcaatact gcgtccagca ccttatcttt cttccgccat
    79621 ttcaccgggt cgtagccttt ggccagaata gccttcactt gttccacggt atgaccaaaa
    79681 ataaagatat tttcttcacc gactttctcg gcgatttcaa cgttcgcacc atccagcgta
    79741 ccgacagtaa gcgcaccatt gagcgccagt ttcatattgc cggtaccgga agcttcttta
    79801 cctgcagtcg aaatttgttc ggagatatcc gccgccggga tcattttttc cgccgccgaa
    79861 acgcaataat ccggcaggaa caccaccttc aacttatcgc caaccagtgg atcgttgttg
    79921 atcacgtcag ccactttgtt gatcgcaaag ataatattct tcgccaggta gtagcccggt
    79981 gccgctttcg cgccgaagag gaagacgcgc ggtacgcgat cagcctgcgg gttttcacga
    80041 atttctttgt acaacgccag aatatgcagc agattcaggt gctggcgttt gtactcatgc
    80101 agacgtttga tctgaatatc gaaaatcgcc tgtggattga tctcaatacc ggtacgaact
    80161 ttcacaaact ccgccagacg gactttattc gcctgcttga tctcgcgata ttgctgacgg
    80221 aatttcgcat catcagcgaa tttttccaga ttgatcagct gatcgagatc gttagcccac
    80281 tctttttgca gtgatttatc caacagagcc gccagtgcag ggttgcactg tttgatccag
    80341 cgacgtgggg taataccgtt ggtgacgtta tggaatttgt tcggccatag ctggtgatat
    80401 tccgggaaca gatctttcac caccagatcc gagtgcagcg ccgcaacacc gttcaccgcg
    80461 aaaccgccaa ccacacacag gttcgccata tgcacttgtt tatcgtgcac caccgccagt
    80521 ttggcccaca ctttttcatc gcccggccag gttttctcca ccagcgtttt aaagcgagta
    80581 ttaatttcgt taataatctg catatggcgc ggcagtaagc ctttcaccag tttcacatcc
    80641 cagcgttcca gcgcttctgg catcagggta tggttggtgt aagcgaaagt tttgctggta
    80701 atggcccagg cgtcatccca gctcatctgg tgctcatcga tcagcacgcg cagcagttct
    80761 ggaatcgcga tagttgggtg ggtatcgttc agctgaataa cttcgtaatc cgccagttcg
    80821 tgcagtttac gccccgccag atgatggcga cgcaaaatat ccgctaccga acaggcacac
    80881 tggaagtact gctgcatcaa gcgcagcttt ttaccggcag tatggttgtc gtttggatag
    80941 agaactttgg tcagtttttc cgcattgatg ccctgctgtt cggcacgcag gaaatcaccg
    81001 tcgttaaatt tagtcagatc aaacggatgc gcgtgcgtcg cctgccacag acgcagcggc
    81061 tgcgccacgc cattacgata gccgacaacg gggagatccc acgcttgacc ggtaatggta
    81121 aactccggct cccagcgtcc gtctttcgtc actttaccgc caatccctac ctgcacatcc
    81181 agtgcttcgt tgtggcggaa ccacgggtag ttactgcgat gccagtcatc cggcgcttca
    81241 acctgtttgc catcgacaaa agactggcgg aacaaaccat attgatagtt cagaccgtaa
    81301 cccgtcgcag actgaccgac agttgccatt gagtcgagga agcacgccgc cagacgtccc
    81361 agaccaccgt tacccagcgc cgggtcgatc tcttcttcca gcaggtccgt cagattgatg
    81421 tcataagcct tcaacgaatc ctgtacatcc tgataccagc cgagattcaa caggttgttg
    81481 cccgtcaggc gaccaatcaa aaactccatt gagatgtagt taacatgtcg ctgattcgcc
    81541 accggcttgg cgaatggctg agcacgcagc atttcggcca gtgcttcact cactgctagc
    81601 caccactggc gaggagtcat ttcagccgca gaatttaagc cataacgctg ccactgacgt
    81661 gaaagcgctt cctgaaattg cttatcgtta aaaataggtt gtgacatagg agttccactt
    81721 ttcttagatt ttcaacacaa cgttatcgct agtttgccag gctcgatgtt gaccttcctc
    81781 atcctgcggg ggattaggca gggaggagtt gcggggatga gcaaggaaat gtgatctcaa
    81841 ccacttaaag ctagtgcaaa ccacaggatt agcagcaaat caatgcaata cagcgcagaa
    81901 aatctgtatc taagtgcaaa aaatggccgt tgcgtatttt caaaaagcgg aaggtaactc
    81961 tataaattaa gtaaaggagt gaaacagttt cataagtaaa atatccagtg tgctccatct
    82021 cattcttaat agatttatta agatcatctt tttagatggc actttcatca ggaatgaaga
    82081 agaaaccctt gcttaaatga atctgatgaa cataagggaa accagtattc acgctggatc
    82141 agcgtcgttt taggtgagtt gttaataaag atttggaatt gtgacacagt gcaaattcag
    82201 acacataaaa aaacgtcatc gcttgcatta gaaaggtttc tggccgacct tataaccatt
    82261 aattacgaag cgcaaaaaaa ataatatttc ctcattttcc acagtgaagt gattaactat
    82321 gctgattccg tcaaaactaa gtcgtccggt tcgactcgac cataccgtgg ttcgtgagcg
    82381 cctgctggct aaactttccg gcgcgaacaa cttccggctg gcgctgatca cgagtcctgc
    82441 gggctacgga aagaccaccc tcatttccca gtgggcggca ggcaaaaacg atatcggctg
    82501 gtactcgctg gatgaaggtg ataaccagca agagcgtttc gccagctatc tcattgccgc
    82561 cgtgcagcag gcaaccaacg gtcactgtgc gatatgtgag acgatggcgc aaaaacggca
    82621 atatgccagc ctgacgtcac tcttcgccca gcttttcatt gagctggcgg aatggcatag
    82681 cccactttat ctggtcatcg atgactatca tctgatcact aatccagtga tccacgagtc
    82741 aatgcgcttc tttattcgcc atcaaccaga aaatctcacc ctggtggtgt tgtcacgcaa
    82801 ccttccgcaa ctgggcattg ccaatctgcg tgttcgtgat caactgctgg aaattggcag
    82861 tcagcaactg gcatttaccc atcaggaagc gaagcagttt tttgattgcc gtctgtcatc
    82921 gccgattgaa gctgcagaaa gcagtcggat ttgcgatgac gtttccggtt gggcgacggc
    82981 actacagcta atcgccctct ccgcccggca gaatacccac tcagcccata agtcggcacg
    83041 ccgcctggcg ggaatcaatg ccagccatct ttcggattat ctggtcgatg aggttttgga
    83101 taacgtcgat ctcgcaacgc gccattttct gttgaaaagc gccattttgc gctcaatgaa
    83161 cgatgccctc atcacccgtg tgaccggcga agaaaacggg caaatgcgtc tcgaagagat
    83221 tgagcgtcag gggctgtttt tacagcggat ggatgatacc ggcgagtggt tctgctatca
    83281 cccgctgttt ggcaacttcc tgcgccagcg ctgccagtgg gaactggcgg cggagctgcc
    83341 ggaaatccac cgtgccgccg cagaaagctg gatggcccag ggatttccca gcgaagcgat
    83401 tcatcatgcg ctggcggcag gcgatgcgct gatgctgcgc gatattctgc ttaatcacgc
    83461 ctggagtttg ttcaaccata gcgaactgtc gctgctggaa gagtcgctta aggccctgcc
    83521 gtgggacagt ttgctggaaa atccgcagtt agtgttattg caggcgtggc taatgcaaag
    83581 ccaacatcgc tatggcgaag ttaacaccct gctagcccgt gctgaacatg aaatcaagga
    83641 catcagagaa ggcaccatgc acgcagaatt taacgctctg cgcgcccagg tggcgattaa
    83701 cgatggtaat ccggatgaag cggaacggct ggcaaaactg gcactggaag agctgccgcc
    83761 gggctggttc tatagccgca ttgtggcaac ctcggtgctg ggtgaagtgc tgcactgcaa
    83821 aggcgaattg acccgctcac tggcgctaat gcagcaaacc gaacagatgg cacgccagca
    83881 cgacgtctgg cactacgcct tatggagttt aatccagcaa agtgaaattc tgtttgccca
    83941 agggttcctg caaaccgcgt gggaaacaca ggaaaaagcg ttccagctga tcaacgagca
    84001 gcatctggaa cagctgccga tgcatgagtt tctggtgcgc atccgagcac aactgttatg
    84061 ggcctgggca cggctggatg aagccgaagc gtcggcgcgt agcgggattg aagtcttgtc
    84121 gtcttatcag ccacagcaac agcttcagtg cctggcaatg ttgattcaat gctcgctggc
    84181 ccgtggtgat ttagataacg cccgtagcca gctgaaccgt ctggaaaacc tgctggggaa
    84241 tggcaaatat cacagcgact ggatctctaa cgccaacaaa gtccgggtga tttactggca
    84301 aatgaccggc gataaagccg ccgctgccaa ctggttgcgt catacggcta aaccggagtt
    84361 tgcgaacaac cacttcctgc aaggtcaatg gcgcaacatt gcccgcgcac aaatcttgct
    84421 gggcgagttt gaacctgcgg aaattgttct cgaagaactc aatgaaaatg cccgcagtct
    84481 gcggttgatg agcgatctca accgtaacct gttgctgctt aatcaactgt actggcaggc
    84541 cggacgtaaa agtgacgccc agcgcgtgtt gctggacgca ttaaaactgg cgaatcgcac
    84601 cggatttatc agccattttg tcatcgaagg cgaagcgatg gcgcaacaac tgcgccagct
    84661 gattcaactc aatacgctgc cggaactgga acagcatcgc gcacagcgta ttctgcgaga
    84721 aatcaatcaa catcatcggc ataaattcgc ccatttcgat gagaatttcg ttgaacgtct
    84781 gctaaatcat ccggaagtgc cagaactgat ccgcaccagc ccgttgaccc aacgtgaatg
    84841 gcaggtcctg gggctgattt actccggtta cagcaatgaa caaattgcgg gcgaactgga
    84901 agtggcggca accaccatca agacgcatat ccgcaatctg taccaaaaac tcggcgtcgc
    84961 ccaccgtcag gctgccgtgc agcacgcgca gaaactgctg aagatgatgg gttatggggt
    85021 gtgagtttag ccggataatg cattggatcc ggtttacatc tttacatcat tcaatactca
    85081 cccgcgttac gccatctgtt tctatcaaac caaaccgcac cggcaagaaa cgctccacca
    85141 ccgcgatatt ggtcagcaga tggcatgaag gatgggcgac cttaaattct cccgcgcccg
    85201 ccagcgccat cggcagtacc agttggtcgg cgagatattc ccccaccgcc gctgggcttg
    85261 ccaggtagcg tttcacctct ttcactaact gcgcggctac cacctcggca ctgacgcgct
    85321 tttcaccgac gacgaaaaag cgttcagtga tattttcact ttcgacttcc agcgagacgg
    85381 tattacccgg cccctggtcg cgcggcaggt tatgaatatt ctgttcatgc agggaaaaac
    85441 tccccgccag tgtagcgatt tcacgctcag caacatggcg cggcacacca gctaatagaa
    85501 cttctccacg catctgcaca atgttcccgc gctcgccaag ttgcaaggtg ttaaacgatg
    85561 ccacgggcga gacttccgtt gccaccacgc cgcctccggc aggataaaaa ccgtgacgta
    85621 acagcgtggt ttgctgatga attcctattt tcgccagcag cggctccagc acccggcgga
    85681 taaaatccgc aggcggggcc gacgggttat cggtgcctcc gctcacttca acacgcgaag
    85741 gtccatcggc aaaccacagc gcgggcagca ccgtttgcag caccagcgta cagcttccgg
    85801 cactaccgat agcaaagcgg tactcgccgc cgcgcacggt gccaggccgg aagaccagac
    85861 gctgcgaccc cagctccgcg ccttccaccg ttgccctaca aatttccgca gccgctttta
    85921 ccgcggtcag atgctggcgc aacagccccg gtttcgcccg cccggcacga atgctggtga
    85981 tggtaaatgg ctggccggtt atcatcgaca ggctcagcgc cgagcgcagg atctgcccgc
    86041 caccttcgcc ctgtgcgcca tccagcgcaa tcatcctttt catcatttat ccttttacgc
    86101 acaccacctg acgcagggta tagataactt ccaccagatc gctttgtgcc gccatcaccg
    86161 catcaatatc tttatacgcc atcgggattt cgtcgatcac ttcggcatct ttacggcatt
    86221 ccacatgcgc ggtggcacga atttgatctt ccacgctgaa cagttttttc gctttagtac
    86281 ggctcattac ccgcccggca ccgtggctgc acgaacagaa cgactcttca tttcccagcc
    86341 cacggacgat aaagcttttt gctcccatcg aaccgggaat aattccatat tgaccagcac
    86401 gcgcagacac cgcgccttta cgcgtcacgt agatctcttc accaaagtgc tgttcttttt
    86461 gcacatagtt gtggtgacag ttgatctctt ccatcgccag cgtttgtggc tgtctgaccg
    86521 ttttctgcgt aatgctctgc aacgccgtta ccacgttttc catcatcgca tcgcggttaa
    86581 ggctggcgaa aagctgcgcc caggccacgg ctcttaggta atcatcaaag tattccgtac
    86641 cttccataaa gtacgccagg tcgcgcgatg gcaacgtttc aagctggtcc tgcatctctt
    86701 tttgtgccaa atcgataaag taagtcccga tggcgttacc aattccgcgt gaaccggagt
    86761 gcagcataat ccacacctgg tccgactcat caaggcagat ttcaataaag tggttacccg
    86821 ttcccagcgt tcccaggtgt ttatagttgt tagtgttaag gaaacgcgga tatttttgcg
    86881 ttaaccactg ataaccggct tcaagctcag cccatttcgc atcgacgtta acaggcgggt
    86941 tttcccatgc gcctttatca cgtttacaac ggccagtggt acgtccgtgc ggcaccgcag
    87001 tttcaatcgc ctgacgcagc tctgccaggt tttcaggcag gtctgccgcc gttaacgcgg
    87061 tacgcagcgc gttcattcca cagccaatat ccacgcccac cgccgccgga ataatcgccc
    87121 ctttggtcgg gatcacgcta ccaatggtgg aacctttacc caggtgtaca tcaggcatta
    87181 ccgcaatatg tttgaaaata aacggcatct tcgccgtatt aataagttgc tgacgcgcat
    87241 cggcttctac cggcacgcct ttggtccaca tttttaccgg ggcattttca gtggtcagta
    87301 attcgtaatt cattttgttt ttctcttttc gttgtttgct gtcgtgataa ttgcaac
//
LOCUS       DAZFWL020000004        86934 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFWL020000004 DAZFWL020000000
VERSION     DAZFWL020000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 86934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.004"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            50..325
                     /gene="ypdI"
                     /locus_tag="ACHKNP_RS01465"
                     /old_locus_tag="pgaptmp_000293"
     CDS             50..325
                     /gene="ypdI"
                     /locus_tag="ACHKNP_RS01465"
                     /old_locus_tag="pgaptmp_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HGU4702538.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(402..644)
                     /gene="yfdY"
                     /locus_tag="ACHKNP_RS01470"
                     /old_locus_tag="pgaptmp_000294"
     CDS             complement(402..644)
                     /gene="yfdY"
                     /locus_tag="ACHKNP_RS01470"
                     /old_locus_tag="pgaptmp_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HGU4702539.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            997..1917
                     /gene="lpxP"
                     /locus_tag="ACHKNP_RS01475"
                     /old_locus_tag="pgaptmp_000295"
     CDS             997..1917
                     /gene="lpxP"
                     /locus_tag="ACHKNP_RS01475"
                     /old_locus_tag="pgaptmp_000295"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HGU4702540.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2273..2344
                     /gene="ypdK"
                     /locus_tag="ACHKNP_RS01480"
                     /old_locus_tag="pgaptmp_000296"
     CDS             2273..2344
                     /gene="ypdK"
                     /locus_tag="ACHKNP_RS01480"
                     /old_locus_tag="pgaptmp_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HGU4702541.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2409..3647)
                     /gene="alaC"
                     /locus_tag="ACHKNP_RS01485"
                     /old_locus_tag="pgaptmp_000297"
     CDS             complement(2409..3647)
                     /gene="alaC"
                     /locus_tag="ACHKNP_RS01485"
                     /old_locus_tag="pgaptmp_000297"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HGU4702542.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            4024..5721
                     /gene="ypdA"
                     /locus_tag="ACHKNP_RS01490"
                     /old_locus_tag="pgaptmp_000298"
     CDS             4024..5721
                     /gene="ypdA"
                     /locus_tag="ACHKNP_RS01490"
                     /old_locus_tag="pgaptmp_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HGU4702543.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5736..6470
                     /gene="ypdB"
                     /locus_tag="ACHKNP_RS01495"
                     /old_locus_tag="pgaptmp_000299"
     CDS             5736..6470
                     /gene="ypdB"
                     /locus_tag="ACHKNP_RS01495"
                     /old_locus_tag="pgaptmp_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HGU4702544.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFANKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6483..7340
                     /gene="ypdC"
                     /locus_tag="ACHKNP_RS01500"
                     /old_locus_tag="pgaptmp_000300"
     CDS             6483..7340
                     /gene="ypdC"
                     /locus_tag="ACHKNP_RS01500"
                     /old_locus_tag="pgaptmp_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4702545.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7343..9838)
                     /gene="ptsP"
                     /locus_tag="ACHKNP_RS01505"
                     /old_locus_tag="pgaptmp_000301"
     CDS             complement(7343..9838)
                     /gene="ptsP"
                     /locus_tag="ACHKNP_RS01505"
                     /old_locus_tag="pgaptmp_000301"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4702546.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILTRASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9863..10900)
                     /gene="ypdE"
                     /locus_tag="ACHKNP_RS01510"
                     /old_locus_tag="pgaptmp_000302"
     CDS             complement(9863..10900)
                     /gene="ypdE"
                     /locus_tag="ACHKNP_RS01510"
                     /old_locus_tag="pgaptmp_000302"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4702547.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10900..11985)
                     /gene="ypdF"
                     /locus_tag="ACHKNP_RS01515"
                     /old_locus_tag="pgaptmp_000303"
     CDS             complement(10900..11985)
                     /gene="ypdF"
                     /locus_tag="ACHKNP_RS01515"
                     /old_locus_tag="pgaptmp_000303"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4702548.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(12000..13247)
                     /gene="fryC"
                     /locus_tag="ACHKNP_RS01520"
                     /old_locus_tag="pgaptmp_000304"
     CDS             complement(12000..13247)
                     /gene="fryC"
                     /locus_tag="ACHKNP_RS01520"
                     /old_locus_tag="pgaptmp_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGU4702549.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKGLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13269..13595)
                     /gene="fryB"
                     /locus_tag="ACHKNP_RS01525"
                     /old_locus_tag="pgaptmp_000305"
     CDS             complement(13269..13595)
                     /gene="fryB"
                     /locus_tag="ACHKNP_RS01525"
                     /old_locus_tag="pgaptmp_000305"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGU4702550.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13814..14779)
                     /gene="glk"
                     /locus_tag="ACHKNP_RS01530"
                     /old_locus_tag="pgaptmp_000306"
     CDS             complement(13814..14779)
                     /gene="glk"
                     /locus_tag="ACHKNP_RS01530"
                     /old_locus_tag="pgaptmp_000306"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HGU4702551.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVYDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14983..16239
                     /gene="yfeO"
                     /locus_tag="ACHKNP_RS01535"
                     /old_locus_tag="pgaptmp_000307"
     CDS             14983..16239
                     /gene="yfeO"
                     /locus_tag="ACHKNP_RS01535"
                     /old_locus_tag="pgaptmp_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HGU4702552.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16354..16680
                     /gene="ypeC"
                     /locus_tag="ACHKNP_RS01540"
                     /old_locus_tag="pgaptmp_000308"
     CDS             16354..16680
                     /gene="ypeC"
                     /locus_tag="ACHKNP_RS01540"
                     /old_locus_tag="pgaptmp_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HGU4702553.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16821..18059)
                     /gene="mntH"
                     /locus_tag="ACHKNP_RS01545"
                     /old_locus_tag="pgaptmp_000309"
     CDS             complement(16821..18059)
                     /gene="mntH"
                     /locus_tag="ACHKNP_RS01545"
                     /old_locus_tag="pgaptmp_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HGU4702554.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18395..19597
                     /gene="nupC"
                     /locus_tag="ACHKNP_RS01550"
                     /old_locus_tag="pgaptmp_000310"
     CDS             18395..19597
                     /gene="nupC"
                     /locus_tag="ACHKNP_RS01550"
                     /old_locus_tag="pgaptmp_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HGU4702555.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19647..21836)
                     /gene="pdeA"
                     /locus_tag="ACHKNP_RS01555"
                     /old_locus_tag="pgaptmp_000311"
     CDS             complement(19647..21836)
                     /gene="pdeA"
                     /locus_tag="ACHKNP_RS01555"
                     /old_locus_tag="pgaptmp_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGU4702556.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGDGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(22044..22119)
                     /locus_tag="ACHKNP_RS01560"
                     /old_locus_tag="pgaptmp_000312"
     tRNA            complement(22044..22119)
                     /locus_tag="ACHKNP_RS01560"
                     /old_locus_tag="pgaptmp_000312"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22084..22086),aa:Ala,seq:ggc)
     gene            complement(22159..22234)
                     /locus_tag="ACHKNP_RS01565"
                     /old_locus_tag="pgaptmp_000313"
     tRNA            complement(22159..22234)
                     /locus_tag="ACHKNP_RS01565"
                     /old_locus_tag="pgaptmp_000313"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22199..22201),aa:Ala,seq:ggc)
     gene            22455..22814
                     /gene="yfeC"
                     /locus_tag="ACHKNP_RS01570"
                     /old_locus_tag="pgaptmp_000314"
     CDS             22455..22814
                     /gene="yfeC"
                     /locus_tag="ACHKNP_RS01570"
                     /old_locus_tag="pgaptmp_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU4702557.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22816..23208
                     /gene="yfeD"
                     /locus_tag="ACHKNP_RS01575"
                     /old_locus_tag="pgaptmp_000315"
     CDS             22816..23208
                     /gene="yfeD"
                     /locus_tag="ACHKNP_RS01575"
                     /old_locus_tag="pgaptmp_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU4702558.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(23260..24675)
                     /gene="gltX"
                     /locus_tag="ACHKNP_RS01580"
                     /old_locus_tag="pgaptmp_000316"
     CDS             complement(23260..24675)
                     /gene="gltX"
                     /locus_tag="ACHKNP_RS01580"
                     /old_locus_tag="pgaptmp_000316"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGU4702559.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            24934..25009
                     /locus_tag="ACHKNP_RS01585"
                     /old_locus_tag="pgaptmp_000317"
     tRNA            24934..25009
                     /locus_tag="ACHKNP_RS01585"
                     /old_locus_tag="pgaptmp_000317"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24967..24969,aa:Val,seq:tac)
     gene            25054..25129
                     /locus_tag="ACHKNP_RS01590"
                     /old_locus_tag="pgaptmp_000318"
     tRNA            25054..25129
                     /locus_tag="ACHKNP_RS01590"
                     /old_locus_tag="pgaptmp_000318"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25087..25089,aa:Val,seq:tac)
     gene            25176..25251
                     /locus_tag="ACHKNP_RS01595"
                     /old_locus_tag="pgaptmp_000319"
     tRNA            25176..25251
                     /locus_tag="ACHKNP_RS01595"
                     /old_locus_tag="pgaptmp_000319"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25209..25211,aa:Val,seq:tac)
     gene            25256..25331
                     /locus_tag="ACHKNP_RS01600"
                     /old_locus_tag="pgaptmp_000320"
     tRNA            25256..25331
                     /locus_tag="ACHKNP_RS01600"
                     /old_locus_tag="pgaptmp_000320"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25289..25291,aa:Lys,seq:ttt)
     gene            25363..25488
                     /locus_tag="ACHKNP_RS01605"
                     /old_locus_tag="pgaptmp_000321"
     ncRNA           25363..25488
                     /ncRNA_class="other"
                     /locus_tag="ACHKNP_RS01605"
                     /old_locus_tag="pgaptmp_000321"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(25597..26481)
                     /gene="xapR"
                     /locus_tag="ACHKNP_RS01610"
                     /old_locus_tag="pgaptmp_000322"
     CDS             complement(25597..26481)
                     /gene="xapR"
                     /locus_tag="ACHKNP_RS01610"
                     /old_locus_tag="pgaptmp_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HGU4702560.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(26522..26680)
                     /locus_tag="ACHKNP_RS01615"
                     /old_locus_tag="pgaptmp_000323"
     CDS             complement(26522..26680)
                     /locus_tag="ACHKNP_RS01615"
                     /old_locus_tag="pgaptmp_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HGU4702561.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(26733..27989)
                     /gene="xapB"
                     /locus_tag="ACHKNP_RS01620"
                     /old_locus_tag="pgaptmp_000324"
     CDS             complement(26733..27989)
                     /gene="xapB"
                     /locus_tag="ACHKNP_RS01620"
                     /old_locus_tag="pgaptmp_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HGU4702562.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            complement(28049..28882)
                     /gene="xapA"
                     /locus_tag="ACHKNP_RS01625"
                     /old_locus_tag="pgaptmp_000325"
     CDS             complement(28049..28882)
                     /gene="xapA"
                     /locus_tag="ACHKNP_RS01625"
                     /old_locus_tag="pgaptmp_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HGU4702563.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            29131..29895
                     /gene="yfeN"
                     /locus_tag="ACHKNP_RS01630"
                     /old_locus_tag="pgaptmp_000326"
     CDS             29131..29895
                     /gene="yfeN"
                     /locus_tag="ACHKNP_RS01630"
                     /old_locus_tag="pgaptmp_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HGU4702564.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(29934..30860)
                     /gene="yfeR"
                     /locus_tag="ACHKNP_RS01635"
                     /old_locus_tag="pgaptmp_000327"
     CDS             complement(29934..30860)
                     /gene="yfeR"
                     /locus_tag="ACHKNP_RS01635"
                     /old_locus_tag="pgaptmp_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4702565.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            30950..31948
                     /gene="yfeH"
                     /locus_tag="ACHKNP_RS01640"
                     /old_locus_tag="pgaptmp_000328"
     CDS             30950..31948
                     /gene="yfeH"
                     /locus_tag="ACHKNP_RS01640"
                     /old_locus_tag="pgaptmp_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HGU4702566.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(31945..32163)
                     /gene="ypeB"
                     /locus_tag="ACHKNP_RS01645"
                     /old_locus_tag="pgaptmp_000329"
     CDS             complement(31945..32163)
                     /gene="ypeB"
                     /locus_tag="ACHKNP_RS01645"
                     /old_locus_tag="pgaptmp_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HGU4702567.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(32165..34180)
                     /gene="ligA"
                     /locus_tag="ACHKNP_RS01650"
                     /old_locus_tag="pgaptmp_000330"
     CDS             complement(32165..34180)
                     /gene="ligA"
                     /locus_tag="ACHKNP_RS01650"
                     /old_locus_tag="pgaptmp_000330"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGU4702568.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(34251..35237)
                     /gene="zipA"
                     /locus_tag="ACHKNP_RS01655"
                     /old_locus_tag="pgaptmp_000331"
     CDS             complement(34251..35237)
                     /gene="zipA"
                     /locus_tag="ACHKNP_RS01655"
                     /old_locus_tag="pgaptmp_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HGU4702569.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            35467..36228
                     /gene="cysZ"
                     /locus_tag="ACHKNP_RS01660"
                     /old_locus_tag="pgaptmp_000332"
     CDS             35467..36228
                     /gene="cysZ"
                     /locus_tag="ACHKNP_RS01660"
                     /old_locus_tag="pgaptmp_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HGU4702570.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36413..37384
                     /gene="cysK"
                     /locus_tag="ACHKNP_RS01665"
                     /old_locus_tag="pgaptmp_000333"
     CDS             36413..37384
                     /gene="cysK"
                     /locus_tag="ACHKNP_RS01665"
                     /old_locus_tag="pgaptmp_000333"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGU4702571.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            37768..38025
                     /gene="ptsH"
                     /locus_tag="ACHKNP_RS01670"
                     /old_locus_tag="pgaptmp_000334"
     CDS             37768..38025
                     /gene="ptsH"
                     /locus_tag="ACHKNP_RS01670"
                     /old_locus_tag="pgaptmp_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HGU4702572.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            38070..39797
                     /gene="ptsI"
                     /locus_tag="ACHKNP_RS01675"
                     /old_locus_tag="pgaptmp_000335"
     CDS             38070..39797
                     /gene="ptsI"
                     /locus_tag="ACHKNP_RS01675"
                     /old_locus_tag="pgaptmp_000335"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HGU4702573.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            39838..40347
                     /gene="crr"
                     /locus_tag="ACHKNP_RS01680"
                     /old_locus_tag="pgaptmp_000336"
     CDS             39838..40347
                     /gene="crr"
                     /locus_tag="ACHKNP_RS01680"
                     /old_locus_tag="pgaptmp_000336"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGU4702574.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(40389..41240)
                     /gene="pdxK"
                     /locus_tag="ACHKNP_RS01685"
                     /old_locus_tag="pgaptmp_000337"
                     /gene_synonym="thiJ"
     CDS             complement(40389..41240)
                     /gene="pdxK"
                     /locus_tag="ACHKNP_RS01685"
                     /old_locus_tag="pgaptmp_000337"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HGU4702575.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            41345..41713
                     /gene="yfeK"
                     /locus_tag="ACHKNP_RS01690"
                     /old_locus_tag="pgaptmp_000338"
     CDS             41345..41713
                     /gene="yfeK"
                     /locus_tag="ACHKNP_RS01690"
                     /old_locus_tag="pgaptmp_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HGU4702576.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(41716..42627)
                     /gene="cysM"
                     /locus_tag="ACHKNP_RS01695"
                     /old_locus_tag="pgaptmp_000339"
     CDS             complement(41716..42627)
                     /gene="cysM"
                     /locus_tag="ACHKNP_RS01695"
                     /old_locus_tag="pgaptmp_000339"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HGU4702577.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(42761..43858)
                     /gene="cysA"
                     /locus_tag="ACHKNP_RS01700"
                     /old_locus_tag="pgaptmp_000340"
     CDS             complement(42761..43858)
                     /gene="cysA"
                     /locus_tag="ACHKNP_RS01700"
                     /old_locus_tag="pgaptmp_000340"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HGU4702578.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(43848..44723)
                     /gene="cysW"
                     /locus_tag="ACHKNP_RS01705"
                     /old_locus_tag="pgaptmp_000341"
     CDS             complement(43848..44723)
                     /gene="cysW"
                     /locus_tag="ACHKNP_RS01705"
                     /old_locus_tag="pgaptmp_000341"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HGU4702579.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQDKRAQQEEHHEH"
     gene            complement(44723..45556)
                     /gene="cysT"
                     /locus_tag="ACHKNP_RS01710"
                     /old_locus_tag="pgaptmp_000342"
     CDS             complement(44723..45556)
                     /gene="cysT"
                     /locus_tag="ACHKNP_RS01710"
                     /old_locus_tag="pgaptmp_000342"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HGU4702580.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(45556..46572)
                     /gene="cysP"
                     /locus_tag="ACHKNP_RS01715"
                     /old_locus_tag="pgaptmp_000343"
     CDS             complement(45556..46572)
                     /gene="cysP"
                     /locus_tag="ACHKNP_RS01715"
                     /old_locus_tag="pgaptmp_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HGU4702581.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(46743..47534)
                     /gene="ucpA"
                     /locus_tag="ACHKNP_RS01720"
                     /old_locus_tag="pgaptmp_000344"
     CDS             complement(46743..47534)
                     /gene="ucpA"
                     /locus_tag="ACHKNP_RS01720"
                     /old_locus_tag="pgaptmp_000344"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HGU4702582.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTDMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(47663..48520)
                     /gene="murR"
                     /locus_tag="ACHKNP_RS01725"
                     /old_locus_tag="pgaptmp_000345"
     CDS             complement(47663..48520)
                     /gene="murR"
                     /locus_tag="ACHKNP_RS01725"
                     /old_locus_tag="pgaptmp_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HGU4702583.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            48684..49580
                     /gene="murQ"
                     /locus_tag="ACHKNP_RS01730"
                     /old_locus_tag="pgaptmp_000346"
     CDS             48684..49580
                     /gene="murQ"
                     /locus_tag="ACHKNP_RS01730"
                     /old_locus_tag="pgaptmp_000346"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HGU4702584.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            49584..51008
                     /gene="murP"
                     /locus_tag="ACHKNP_RS01735"
                     /old_locus_tag="pgaptmp_000347"
     CDS             49584..51008
                     /gene="murP"
                     /locus_tag="ACHKNP_RS01735"
                     /old_locus_tag="pgaptmp_000347"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HGU4702585.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            51013..52317
                     /gene="pbp4b"
                     /locus_tag="ACHKNP_RS01740"
                     /old_locus_tag="pgaptmp_000348"
     CDS             51013..52317
                     /gene="pbp4b"
                     /locus_tag="ACHKNP_RS01740"
                     /old_locus_tag="pgaptmp_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HGU4702586.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(52375..53274)
                     /gene="yfeX"
                     /locus_tag="ACHKNP_RS01745"
                     /old_locus_tag="pgaptmp_000349"
     CDS             complement(52375..53274)
                     /gene="yfeX"
                     /locus_tag="ACHKNP_RS01745"
                     /old_locus_tag="pgaptmp_000349"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HGU4702587.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(53370..53945)
                     /gene="yfeY"
                     /locus_tag="ACHKNP_RS01750"
                     /old_locus_tag="pgaptmp_000350"
     CDS             complement(53370..53945)
                     /gene="yfeY"
                     /locus_tag="ACHKNP_RS01750"
                     /old_locus_tag="pgaptmp_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HGU4702588.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(54006..54455)
                     /gene="yfeZ"
                     /locus_tag="ACHKNP_RS01755"
                     /old_locus_tag="pgaptmp_000351"
     CDS             complement(54006..54455)
                     /gene="yfeZ"
                     /locus_tag="ACHKNP_RS01755"
                     /old_locus_tag="pgaptmp_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HGU4702589.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(54442..54867)
                     /gene="ypeA"
                     /locus_tag="ACHKNP_RS01760"
                     /old_locus_tag="pgaptmp_000352"
     CDS             complement(54442..54867)
                     /gene="ypeA"
                     /locus_tag="ACHKNP_RS01760"
                     /old_locus_tag="pgaptmp_000352"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGU4702590.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            55081..55950
                     /gene="amiA"
                     /locus_tag="ACHKNP_RS01765"
                     /old_locus_tag="pgaptmp_000353"
     CDS             55081..55950
                     /gene="amiA"
                     /locus_tag="ACHKNP_RS01765"
                     /old_locus_tag="pgaptmp_000353"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HGU4702591.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            55954..56853
                     /gene="hemF"
                     /locus_tag="ACHKNP_RS01770"
                     /old_locus_tag="pgaptmp_000354"
     CDS             55954..56853
                     /gene="hemF"
                     /locus_tag="ACHKNP_RS01770"
                     /old_locus_tag="pgaptmp_000354"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HGU4702592.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERECNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(56859..57911)
                     /gene="eutR"
                     /locus_tag="ACHKNP_RS01775"
                     /old_locus_tag="pgaptmp_000355"
     CDS             complement(56859..57911)
                     /gene="eutR"
                     /locus_tag="ACHKNP_RS01775"
                     /old_locus_tag="pgaptmp_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HGU4702593.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHCEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(57957..58457)
                     /gene="eutK"
                     /locus_tag="ACHKNP_RS01780"
                     /old_locus_tag="pgaptmp_000356"
     CDS             complement(57957..58457)
                     /gene="eutK"
                     /locus_tag="ACHKNP_RS01780"
                     /old_locus_tag="pgaptmp_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HGU4702594.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(58470..59129)
                     /gene="eutL"
                     /locus_tag="ACHKNP_RS01785"
                     /old_locus_tag="pgaptmp_000357"
     CDS             complement(58470..59129)
                     /gene="eutL"
                     /locus_tag="ACHKNP_RS01785"
                     /old_locus_tag="pgaptmp_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HGU4702595.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(59139..60026)
                     /gene="eutC"
                     /locus_tag="ACHKNP_RS01790"
                     /old_locus_tag="pgaptmp_000358"
     CDS             complement(59139..60026)
                     /gene="eutC"
                     /locus_tag="ACHKNP_RS01790"
                     /old_locus_tag="pgaptmp_000358"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HGU4702596.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(60047..61408)
                     /gene="eutB"
                     /locus_tag="ACHKNP_RS01795"
                     /old_locus_tag="pgaptmp_000359"
     CDS             complement(60047..61408)
                     /gene="eutB"
                     /locus_tag="ACHKNP_RS01795"
                     /old_locus_tag="pgaptmp_000359"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HGU4702597.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(61420..62823)
                     /gene="eutA"
                     /locus_tag="ACHKNP_RS01800"
                     /old_locus_tag="pgaptmp_000360"
     CDS             complement(61420..62823)
                     /gene="eutA"
                     /locus_tag="ACHKNP_RS01800"
                     /old_locus_tag="pgaptmp_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HGU4702598.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLMETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(62820..64046)
                     /gene="eutH"
                     /locus_tag="ACHKNP_RS01805"
                     /old_locus_tag="pgaptmp_000361"
     CDS             complement(62820..64046)
                     /gene="eutH"
                     /locus_tag="ACHKNP_RS01805"
                     /old_locus_tag="pgaptmp_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HGU4702599.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(64163..65350)
                     /gene="eutG"
                     /locus_tag="ACHKNP_RS01810"
                     /old_locus_tag="pgaptmp_000362"
     CDS             complement(64163..65350)
                     /gene="eutG"
                     /locus_tag="ACHKNP_RS01810"
                     /old_locus_tag="pgaptmp_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HGU4702600.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQSRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(65340..66176)
                     /gene="eutJ"
                     /locus_tag="ACHKNP_RS01815"
                     /old_locus_tag="pgaptmp_000363"
     CDS             complement(65340..66176)
                     /gene="eutJ"
                     /locus_tag="ACHKNP_RS01815"
                     /old_locus_tag="pgaptmp_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HGU4702601.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(66187..67590)
                     /gene="eutE"
                     /locus_tag="ACHKNP_RS01820"
                     /old_locus_tag="pgaptmp_000364"
     CDS             complement(66187..67590)
                     /gene="eutE"
                     /locus_tag="ACHKNP_RS01820"
                     /old_locus_tag="pgaptmp_000364"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGU4702602.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(67602..67889)
                     /gene="eutN"
                     /locus_tag="ACHKNP_RS01825"
                     /old_locus_tag="pgaptmp_000365"
     CDS             complement(67602..67889)
                     /gene="eutN"
                     /locus_tag="ACHKNP_RS01825"
                     /old_locus_tag="pgaptmp_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HGU4702603.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(67996..68289)
                     /gene="eutM"
                     /locus_tag="ACHKNP_RS01830"
                     /old_locus_tag="pgaptmp_000366"
     CDS             complement(67996..68289)
                     /gene="eutM"
                     /locus_tag="ACHKNP_RS01830"
                     /old_locus_tag="pgaptmp_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HGU4702604.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(68328..69344)
                     /gene="pta"
                     /locus_tag="ACHKNP_RS01835"
                     /old_locus_tag="pgaptmp_000367"
     CDS             complement(68328..69344)
                     /gene="pta"
                     /locus_tag="ACHKNP_RS01835"
                     /old_locus_tag="pgaptmp_000367"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU4702605.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(69341..70144)
                     /gene="eutT"
                     /locus_tag="ACHKNP_RS01840"
                     /old_locus_tag="pgaptmp_000368"
     CDS             complement(69341..70144)
                     /gene="eutT"
                     /locus_tag="ACHKNP_RS01840"
                     /old_locus_tag="pgaptmp_000368"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HGU4702606.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(70141..70842)
                     /gene="eutQ"
                     /locus_tag="ACHKNP_RS01845"
                     /old_locus_tag="pgaptmp_000369"
     CDS             complement(70141..70842)
                     /gene="eutQ"
                     /locus_tag="ACHKNP_RS01845"
                     /old_locus_tag="pgaptmp_000369"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HGU4702607.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(70817..71296)
                     /gene="eutP"
                     /locus_tag="ACHKNP_RS01850"
                     /old_locus_tag="pgaptmp_000370"
     CDS             complement(70817..71296)
                     /gene="eutP"
                     /locus_tag="ACHKNP_RS01850"
                     /old_locus_tag="pgaptmp_000370"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HGU4702608.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(71309..71644)
                     /gene="eutS"
                     /locus_tag="ACHKNP_RS01855"
                     /old_locus_tag="pgaptmp_000371"
     CDS             complement(71309..71644)
                     /gene="eutS"
                     /locus_tag="ACHKNP_RS01855"
                     /old_locus_tag="pgaptmp_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HGU4702609.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(71937..74216)
                     /gene="maeB"
                     /locus_tag="ACHKNP_RS01860"
                     /old_locus_tag="pgaptmp_000372"
     CDS             complement(71937..74216)
                     /gene="maeB"
                     /locus_tag="ACHKNP_RS01860"
                     /old_locus_tag="pgaptmp_000372"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HGU4702610.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            74505..75455
                     /gene="tal"
                     /locus_tag="ACHKNP_RS01865"
                     /old_locus_tag="pgaptmp_000373"
     CDS             74505..75455
                     /gene="tal"
                     /locus_tag="ACHKNP_RS01865"
                     /old_locus_tag="pgaptmp_000373"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU4702611.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            75475..77478
                     /gene="tkt"
                     /locus_tag="ACHKNP_RS01870"
                     /old_locus_tag="pgaptmp_000374"
     CDS             75475..77478
                     /gene="tkt"
                     /locus_tag="ACHKNP_RS01870"
                     /old_locus_tag="pgaptmp_000374"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU4702612.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSMKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGVIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(77574..78617)
                     /gene="ypfG"
                     /locus_tag="ACHKNP_RS01875"
                     /old_locus_tag="pgaptmp_000375"
     CDS             complement(77574..78617)
                     /gene="ypfG"
                     /locus_tag="ACHKNP_RS01875"
                     /old_locus_tag="pgaptmp_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HGU4702613.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQVIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(78743..79318)
                     /gene="nudK"
                     /locus_tag="ACHKNP_RS01880"
                     /old_locus_tag="pgaptmp_000376"
     CDS             complement(78743..79318)
                     /gene="nudK"
                     /locus_tag="ACHKNP_RS01880"
                     /old_locus_tag="pgaptmp_000376"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HGU4702614.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(79386..81365)
                     /gene="aegA"
                     /locus_tag="ACHKNP_RS01885"
                     /old_locus_tag="pgaptmp_000377"
     CDS             complement(79386..81365)
                     /gene="aegA"
                     /locus_tag="ACHKNP_RS01885"
                     /old_locus_tag="pgaptmp_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HGU4702615.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKPRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMNAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            81571..83271
                     /gene="narQ"
                     /locus_tag="ACHKNP_RS01890"
                     /old_locus_tag="pgaptmp_000378"
     CDS             81571..83271
                     /gene="narQ"
                     /locus_tag="ACHKNP_RS01890"
                     /old_locus_tag="pgaptmp_000378"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HGU4702616.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            83435..86548
                     /gene="acrD"
                     /locus_tag="ACHKNP_RS01895"
                     /old_locus_tag="pgaptmp_000379"
     CDS             83435..86548
                     /gene="acrD"
                     /locus_tag="ACHKNP_RS01895"
                     /old_locus_tag="pgaptmp_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HGU4702617.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(86647..86706)
                     /gene="ypfM"
                     /locus_tag="ACHKNP_RS01900"
                     /old_locus_tag="pgaptmp_000380"
     CDS             complement(86647..86706)
                     /gene="ypfM"
                     /locus_tag="ACHKNP_RS01900"
                     /old_locus_tag="pgaptmp_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HGU4702618.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        20789 a        23668 c        21927 g        20550 t
ORIGIN      
        1 agccataaca tcgtaggttc tcagtgaacc attttcagga taactttcta tgaaagtaaa
       61 cttaatactt ttcagcttat ttttattggt ctctattatg gcatgcaatg tttttgcatt
      121 ttccatttct ggtggtgtaa gtgagaggag ctataaagag actgaaaaaa catcagcgat
      181 gacaaccaca cactctacaa aacttcagcc atcacaggcg attttgttta agatgagaga
      241 agatgcgcca ccattaaacc tcacagaaga aatcacgcct acttatccga caaaggcgaa
      301 ttatcttatt catcctgtgc gatagcgtct gtcacatggc aggtgaacaa tcatgtctga
      361 tgctgatgtg aacacctgcc caaatgagcc atcctgaccc actatcgcct gccatacccc
      421 accataaagc gaatcatcaa gccacaagcc ggggcaagaa acataaatat ccccgtcact
      481 agttcattac cgccagttaa ccacatggtg aagtaataaa tgagtgcagc gaggatcacc
      541 atgccgaaga aagaagagac cgccgatacc acgttcagta cctgaccgat gtagccgctt
      601 acgcagacaa tacacagggc gagaaacatc cataaataaa tcatagctca acctcctgcc
      661 gcgctaaaaa acctgtaact tcagtcactt taatagtaag acaatcctta acctctggca
      721 atttttgcta agcatcaatg aaaacagcat gttaaatgca agactgttgt gtacggaaaa
      781 atatttactt tgcacgatta ataatcagta gctgaaagca gtcagcgagt gataaagtac
      841 agataccgca ttcatgctcc tcgtggttat gtcctgacta gtctttacac tctttacagg
      901 aaccattgtc gtacatgatg gcccaaccaa ttgagtgtac cgctgtcgac atggaatctt
      961 ctgcgataca acaattcgta tctacagaag gtaactatgt ttccacaatg caaattttcc
     1021 cgcgagtttc tacatcctcg ctactggctc acatggtttg ggcttggtgt actctggctt
     1081 tgggtacagc ttccttatcc tgttctctgc tttctcggca cgcgtattgg cgcaatggcg
     1141 cgaccattcc tgaaacgtcg tgaatctatc gcccgtaaaa acctggaact ttgtttcccg
     1201 cagcattctg cggaagaacg cgagaagatg attgccgaaa actttcgttc actcggcatg
     1261 gcgctggtag aaaccggcat ggcatggttc tggcccgaca gtcgcgtacg taaatggttt
     1321 gatgttgaag ggttggataa ccttaaacgc gcacaaatgc aaaatcgcgg cgtaatggtt
     1381 gtcggcgtcc attttatgtc gctggaactg ggcggccgcg tgatgggact gtgccaacca
     1441 atgatggcta cctatcgtcc acataataat cagctgatgg aatgggtgca gacccgtggg
     1501 cgcatgcgct ctaacaaagc gatgatcggc agaaataatc tgcgcggcat tgtcggtgca
     1561 ctgaagaaag gtgaagcggt atggtttgct cccgatcagg attatggtcg taaaggcagc
     1621 tccttcgcgc cgttctttgc ggtggaaaat gtcgccacaa ccaatggcac ctatgttctc
     1681 tcccgtctct ctggcgcagc catgttgacc gtaacgatgg taagaaaagc ggattacagc
     1741 ggatatcgtt tgttcatcac cccagagatg gaaggctacc cgacagatga aaatcaagcc
     1801 gctgcctata tgaacaagat tatcgagaaa gagatcatgc gcgcaccgga gcagtacctc
     1861 tggatccacc gtcgctttaa aacgcgcccg gtgggagaat cgtcgttgta catttaacaa
     1921 actgctctta ctctttccct cctcaaatcg gccataataa cctcatggtt tttatggctt
     1981 ttttcttaag gttttttcct tcgctgaata tctaattatt accgtcagaa attaaactgt
     2041 cgccgatatg agacacttgt aagttactaa aagtaaataa gaaaatgtct cttgtctcgt
     2101 ttcagtttta ggcgtacatt agcgccgtct cgagcagttt agctcagaat tctggggtga
     2161 tgcccacggt aaaatacagg gcttttaagg tcgctttgcc cggtgtcaac tcactgtatc
     2221 aggtgtaatg aagtcattca ggcgtaacag taattacgcg gagagatgta aagtgaaata
     2281 tttctttatg ggcatttctt ttatggtcat cgtttgggcc ggtacttttg ccctgatgat
     2341 ctaaaagcaa actgtgcaat gaaaaacagg agccatcagg ctcctgtttg catttctgac
     2401 gacggtgttt attccgcgtt ttcgtgaata tgtttgctgc tggcgggtag taaaccgtcg
     2461 gcacggaaca tcgctttaat cccacgaatc gcctgacgaa tacggtcgcg gttttcaatc
     2521 agtgcaaagc gaacatgcgt atcaccatag tcgccaaagc caatccccgg cgagacacaa
     2581 accttcgctt cgttaaggag cttcttggca aattccagcg atcccatggc cgcatatggt
     2641 tccgggattt tcgcccagac atacatcgaa gccttcggca tttcaaccat ccagcccgct
     2701 tcatgcagcc ctttaaccaa tacatcacgg cggcgtttgt actgttcggc aatgtcgcgc
     2761 acgcactgtt gatcgccctc cagcgccgca atcgccgcca cctgcaacgg cgtaaaggtg
     2821 ccgtaatcgt gatagctttt aatacgagcc agggcgctga ccagcgtttt gttgccaacc
     2881 ataaagccga tacgccagcc cgccatgttg tagcttttcg acagcgtaaa gaactcgact
     2941 gccacatcgc gtgcacccgg tacctgcatg attgacggcg ctttccagcc atcgtagacg
     3001 atatcggcat aggccaggtc gtggaccacc agcacatcgt agcgtttcgc cagcgccaca
     3061 accttttcga agaactccag ctccacgcat tgcgcggttg gattcgacgg gaagccgagg
     3121 atcatcatct tcggtttcgg ataactttca cgaatggcgc gctccagttc gttgaagaaa
     3181 tcgacacctt ccaccagcgg cactgagcgt acctgcgccc cggcaatcac cgcgccataa
     3241 atatggatcg ggtaacttgg attcggcacc agcaccgtgt caccatgatc cagcgtcgcc
     3301 agcatcagat gcgccaggcc ctctttcgaa ccaatagtga cgatggcttc tgattccggg
     3361 tcgatttcaa cgtcgtagcg atcctgatac cagcgggaaa tggcgcgacg taaccgcgga
     3421 atgccgcgtg aagtggagta accatgcgtg tccgggcgct gggccacagt acataatttt
     3481 tcgacgatat gcggcggagt cgcaccgtcc gggttaccca tgctgaaatc gataatatct
     3541 tcgccgcgcc gacgcgcagc cattttcagt tcagcggtga tgttaaaaac gtagggcggg
     3601 agacgatcaa tgcgcgtaaa gcgacgttca gggcgagtgt cagccataat ttcctcagat
     3661 taacgttagc gcccggaccg tccgagcgac gctgccacaa tggtggctgt tttgaaaata
     3721 gcctgattaa tttctgtctg tccagcgttt tagaacattt ttatcaatgg ctttaaatga
     3781 ggaactccac ttcaaaaaca ggaaattttt ctacgctaac aagaaaatta ccaccaattt
     3841 tattaacatt ataatttcaa taaatttacc aaccagaagt gcattatcaa gagatacatt
     3901 gctaatacac catttatctt tcaatcccct tttaaattta tggtttttcc tcaaaaacgg
     3961 cctgctggtc atcagccttc aggttaccta tcatagaggt ttaatcctta ttcagagtca
     4021 cccgtgcacg aaatattcaa catgctgctg gcggtcttcg atcgggcagc gttaatgctt
     4081 atctgcctgt tctttctcat ccgtatccgc ctgtttcgcg aactgttgca caagtcggcg
     4141 cactccccaa aagaattgct cgccgttacc gccattttct cgctgttcgc cctgttcagc
     4201 acctggtccg gcgttcccgt agaaggctcg ctggtgaacg tacgtattat cgcggtgatg
     4261 tccggcggga ttctgtttgg cccgtgggta ggcatcatta ccggcgtgat tgcgggtatt
     4321 caccggtatt taattgatat cggcggcgtg acggcgatcc cctgctttat caccagcatt
     4381 ctggcgggtt gtatatcggg ctggatcaac ctgaaaatcc ccaaagcaca gcgctggcgc
     4441 gtcggtattc tcggcggcat gttgtgtgag acgttgacca tgattctggt aattgtctgg
     4501 gcaccaacta ccgcgttggg gatcgatatc gtctctaaaa tcggcattcc aatgatcctc
     4561 ggtagcgtct gtatcggctt tattgtgctt ctggtgcaaa gcgttgaggg cgaaaaagag
     4621 gccagcgccg cgcggcaggc caagctggcg ctggatatcg ccaacaaaac gctaccgctg
     4681 tttcgccatg tcaatagcga gtcattacgc aaggtctgcg aaattatccg cgatgacatt
     4741 cacgccgatg cggtggcgat tactaatacc gatcatgtgc tggcctatgt tggcgtgggt
     4801 gaacataact atcagaatgg cgatgacttc attagcccga ctacccgtca ggcgatgaat
     4861 tacggaaaaa tcatcattaa aaacaatgat gaagcccacc gcacaccaga gattcattcc
     4921 atgctggtga tcccattgtg ggagaaaggg gtcgtgaccg gaacgctgaa aatttactac
     4981 tgccacgcgc atcagatcac ctcgtcatta caggaaatgg cggtcggtct gtcgcaaatc
     5041 atctccacgc aactggaggt ttcacgcgcc gagcagctac gtgaaatggc aaataaggca
     5101 gagcttcgcg ccctgcaaag caaaattaat ccccattttc tgtttaacgc tctgaacgct
     5161 atttcatcgt caatccgtct gaatccggat accgctcgcc agttgatctt taatctgtcg
     5221 cgttatctgc gctataacat tgaattaaaa gacgatgagc aaatcgatat caaaaaagag
     5281 ctgtatcaaa ttaaagacta tattgccatt gagcaggccc gctttggtga caagctgacg
     5341 gttatctatg atattgatga agaggtgaat tgctgcattc ccagcctgct gatccagccg
     5401 ttggtggaga acgccattgt ccacggtatt cagccttgca aaggtaaagg cgttgtcacc
     5461 atcagcgttg cagagtgcgg aaatcgggta cgcattgcgg tgcgagatac cgggcacggc
     5521 atcgatccaa aggtgattga gcgggtcgaa gcgaatgaaa tgccgggcaa taaaattggc
     5581 ctgctgaatg tccatcatcg cgtgaagtta ttgtatggcg aggggctgca tatccgccgc
     5641 ctggagccgg ggacggaaat tgcgttttac attcctaacc aacgcacccc agtcgcctca
     5701 caggctacgt tattgctttg agccggagtg atattgtgaa agtcatcatt gttgaagacg
     5761 aattcctggc acaacaggaa ctgagctggc taattaaaga gcacagccag atggagattg
     5821 tcggcacctt tgacgacggt ctggacgtgt tgaagttttt gcagcataac cgcgtcgacg
     5881 ccatttttct ggatatcaat attccgtcgc tggatggcgt gttgctggcg caaaacatca
     5941 gccagttcgc caataaaccg tttattgtgt tcatcaccgc gtggaaagaa catgcggtag
     6001 aagcgtttga actggaggcg tttgactaca ttctcaaacc gtatcaggag tcacgtatta
     6061 ccgggatgct gcaaaaactg gaagcggcct ggcaacaaca gcagaccagc agtacgcctg
     6121 ccgcgacggt aacgcgtgag aatgacacca ttaatctggt gaaagatgag cgaataatcg
     6181 tcacgccaat taacgatatc tattacgccg aagcgcacga gaaaatgacc tttgtctata
     6241 cgcggcgtga atcctacgta atgccgatga acattaccga attttgcagc aaactgccgc
     6301 cgtcgcattt tttccgctgc catcgctcat tttgtgtcaa tctgaacaaa atacgcgaaa
     6361 tcgaaccgtg gtttaataac acctacattc tgcgactgaa agatctggat tttgaagtgc
     6421 cggtcagtcg cagcaaagtg aaagaatttc gccagttaat gcatctttaa tcgggaactt
     6481 tcatgaaagc accaggcttg cccgccgatc aacaattttt cgccgatctg ttcagcggcc
     6541 tggtgcttaa cccgcaacta ctggggcgcg tctggtttgc cagccagcct gcctcgttac
     6601 cggtgggcag tttatgtatt gattttcccc gtctggatat cgtgctgcgc ggcgaatacg
     6661 gcaacctgct ggaagcaaag cagcagcgaa tggtggaagg agaaatgctg tttattccgg
     6721 cacgcgcggc taatttaccg atcaacaaca aaccggtgat gctgttaagc ctggtgttcg
     6781 caccgacctg gcttgggtta tcgttttacg atagccgcac cacgtcgttg ttgcatcctg
     6841 ctcgccagat ccagcttccc agcctgcaac gcggtgaagg tgaagcgatg cttaccgccc
     6901 tcacccatct tagccgttcg ccgctggagc agaatatcat tcagccactg gtgttaagtt
     6961 tgctgcatct ttgccgcaac gtggtgaata tgccgccggg taattcgcaa ccgcgcggcg
     7021 attttctcta tcacagcatt tgtaactggg ttcaggataa ttatgcccag ccgctcaccc
     7081 gcgagagcgt ggcgcagttt tttaatatca cgcccaatca tctgtcaaaa ctgtttgctc
     7141 agcatggaac gatgcgtttt atcgagtatg tgcgttgggt gcgaatggcg aaggcgagga
     7201 tgattttgca gaaatatcat ctgtcaattc atgaagtggc acagcgttgc ggttttccgg
     7261 atagcgacta tttttgtcgc gttttccggc gtcagtttgg tctgacgccg ggagagtaca
     7321 gcgcccgttt tcagggctaa cgtcagaagg ttaattctgt ttccagcagc gtcaggatac
     7381 tttgtgcatc ttgcgcggca aacaacgact ggcggaagtt tttattcacc agtttacgcg
     7441 ccagctgcga gaagactttc acatgattca tcccttcgtt agcacccagc gtcagcatga
     7501 tcaccagttc gacttcgccc atttctgact gccagtcaac cggtttcgcc agccgggcaa
     7561 tgctgatact ggaatgacgg atccactgag atttggtgtg cgggatcgct acgccaaaac
     7621 caacgccggt ggtgacaatc tcttcccgct gccagacatc ttcttccagc tcaaacggat
     7681 gttcagtgcg cccgttaacg ccgaggttgc cgcacaggaa ctggatcgcc tgctctttat
     7741 tgctaaaatc ctgatcaaca aagatattct ccagcgccag cagtgggcga acgtcttctt
     7801 ccggcgtaaa ggcggtgagt aacgcttcaa tttcctgcgc actgcggcat tcacatgcct
     7861 gacgcgccag ttcccgacac gcctcgctat ccagttgacg aagctggctt ttcaccgccg
     7921 gaatacgcgg gctactcata ctcagctcat ccaggcccag cccaagcagt agcggcagat
     7981 aacggctttc accgcccagt tcaccgcaaa tgcctaccca tttgccccgc tgatgcgcag
     8041 tggtaacaat ctgctgcaac atgcgcagga acgatggcgt aatcgggtta tacagcggcg
     8101 atacgcgcgg gttattacga tcgaccgcat acagatactg ggtcatatcg ttggagccga
     8161 tactgaagaa atcgacctca tcgcagaagt gatcgatgat gtagcacacc gacggcactt
     8221 ccaccatgat ccccagcgta atcgtctctg catgacgcag gccatcgcgc ttaagctcaa
     8281 cgatcgcttt ttggatctcg cccttcaccc ataagatctg atcgagactg tggaccatcg
     8341 ggatcatcaa ctgggcgttg ccaaaactgg cggcgcgcaa aatcgcccgt agttgagtgc
     8401 ggaacaggcc agcaaattcc gggtaaatac gtaccgcgcg atagccgagg aacgggttct
     8461 cttcctgggg aatattcaga taaggaatgc ttttatcgcc accgatgtcc attgtgcgga
     8521 agataatcgg cttgtcgccc gctgccagta gcacctgctg gtaggcttca aattgctcct
     8581 gctcgtccgg cgcgctgtcg cgatccatat agagcatttc agtacggaac agtccaacgc
     8641 cttccgcgcc gttggcaaac gcgcctggcg cttccagagc ggtgccgata ttcgccgcaa
     8701 tatcaatacg cttgttatca cgggaatagg ccagctgcgc ggcggcctgc gcctgttgtt
     8761 tttggcgttt atccgccagc gtctgtgcga cctgataata accgctcacc gcgtcattcg
     8821 ggttaatcgc cagcacgccg cactgggcgt caagcaccgc cggttgcccg gcataacggg
     8881 caatcgcatc cagcggcagg ccgctcagta ccgggatcgc cgaggcgcgg gtcagaatca
     8941 gggtatgcga ggtgcgccca gttttctcca gaatcatgcc tgcaagattt tttaaatcga
     9001 ggctcaaaaa ctggcttggg gttaaatcct cggctaccag aatagtcggt ttttccagca
     9061 ccagattgtt gcgcggcttc agttccggcc aggtgatatg cagcaactgt tcgctaatat
     9121 cgcgaatgtc gctaacacgt tcgcgcagat aatcactggc agaagcagaa agtttggcgc
     9181 aaacctgctc catattgctg atgatcgccg cccccagccc ttgatgctgt tctgtcatca
     9241 gacgacggat attgcccgcg aattcatcat cctgaatcag cgacaaatgg gcgctgagga
     9301 tagttttgct ttcgccgtca cgctcacgca attgctggtt cagttgctcg gcaagcgttg
     9361 ccaggctgtg ctccagccgg gtggaatctt gcgcactggc ggggattgcc cgataactgt
     9421 cgaggctgtc gctttgtaac agggtcagcg tacccacgcc gacgccgctt gccagcacat
     9481 tgccgtacag taaatccggg ttaaggcggc ttaatgaacg cggcagcgga tgcgccgtca
     9541 gttctgcctg cgtcggctga acgctgtcgc tgtcgataaa gcgcaccagg atgtactctt
     9601 ccagcacgcg ccgcgcctgc tcttcatcgc tgccgctaat gttcaggctg caactgtcat
     9661 taaataaggt gccagttcca atcaacgcca gcgagctttt agcatcggcc tttgcgttct
     9721 ggcgatggtt gaaaaaggtg atttcgcttt gccactggct gcactgttct ttaagttccc
     9781 acgccggacg agcatgtaga ccgttaggca aaggacagag aaattgaatc gttaacataa
     9841 ccgctccggg ttaatcagga gatcatctga aatccgtcag ttgaacaacc gtctcacgcg
     9901 taagacgttg aataagggca gataaaagtt gctccatctg caaaatgtcg cggcaatcgg
     9961 caatcgatgc cgcgcaatgt ccgtggcggg tggcaggccc cattaccact gtgggtacgc
    10021 cagtaccggt taagtgcacc gctccgccgt ccgtgccgcc gttgctgaac atatctgcct
    10081 gcaacggtac gccaatttca gccgccacgg tttcgatcca ggcggtaagt tttggcggcg
    10141 caatcagtga cttgtcgctt aacaccagca tcgggccgtt accaatctgg cgatggttag
    10201 ccgcgccata atcaaagttt ttcgcccagc aggcggtatc cagcacaatg gcgacgtccg
    10261 gcgacaccgc gcgggtagcg gtttgcccac cgcgtaatcc cacctcttcg ctggaacttg
    10321 ccaccagcca cacctccgca ggcagttcgg catcgtgcag ctcacgtagt aacgtcacca
    10381 gcagatagca accgaggcga tcatcaaagg ctttccccat cacccgctgg tgagggagaa
    10441 cctgaaaagt tgtatcaaag gtgacgcgat cgcctggacg aattcccgcc tgcatcactt
    10501 cgtcatacga gcgcgcacca atgtccacgc gcatggcgct gacgtcattc ccctgccggt
    10561 cgccgtcaag caggcctgga attttgcact cttcacgggt ggtgatgcgc accggctgca
    10621 gctggcgggc agccatgcgc acgttgccaa ccggcagcac atcaattgcc ccttcgcggg
    10681 agatgctacg caccataaat cccacttcgt ccatatgggc gcagatcatc accttcggac
    10741 ctgtcgattc attgaggcgg atcagcaccg atcccagacc atcaaatcgc acttcttttt
    10801 gcaggcgatc cgcttcttcc agcaggatct gccgcacttc ctgttccgag gaggcgatcg
    10861 catctgcctc gctcaacgct tttaatagcg ataaatccat tatgcctctc ccgtgagcaa
    10921 cactgttttc ggcatggcgt agagcacttc tgcgccttgc ggggtaacca gcacaacatc
    10981 ttcgatgcgc acgccccctt gccctggcaa ataaatcccc ggctccacgg tcagtaacat
    11041 gcctggctgt agcgtcgtgg tgtcccgcgg tgaaaaacgc ggatcttcat gaacttcaat
    11101 gccgatagca tgaccggtgt tatgaccgaa atagtggcta aaacctgcct cggtaatcac
    11161 ttggcgcgcg gcatcgtcaa cctgctggca gcgcacgccg gggcgaattg cagagattgc
    11221 tgcgagctgt gcctgcagga caatctggta gacgttaaac agcgggtgag attcggcgct
    11281 caccccttcg ccattcacca gcaaggtgcg cgtcatatca gagcagtagc cctgatacag
    11341 cgcaccaaaa tcgagagtga caaactcgcc cgctgcaaca atcttgtcgc tggctttgcc
    11401 gtgcggcagc gccccgcgcc agccgctggc gacaatggtg tcaaaagagg ctttttctgc
    11461 gccctgctgg cgcataaacc actccagttc agcggctatc tcgcgctcgc tcatccccgc
    11521 ctgaataaag cggcgaatat gctccgctcc gcgatcggca atcccacagg cgaggcggat
    11581 tatctccacc tcctctggcg ttttgatttg ccgcagcaca tccggcgtgg cgctaaccag
    11641 tttcgcattg agttcagact tccagcggtg cgcggtttcc caactcacct gctggccttc
    11701 aaaaccgagc gtttgcaact gctcatcggc aatgatttga ttgacgatag tggtaagcgt
    11761 gttcgtcgcg tcaagcaaat gcagctggta gccttgcgca cgggcttcca cctcaacgaa
    11821 atagcgcgaa tctaccagaa tgtgcgcact ttcacggcta ataaccacat agcctgatcc
    11881 ggtggagatc cccagatgcg gctgtttgtt ctgccgtgag gagagaagca ctgcatccag
    11941 ttgttgcgcc ttaagccagt cgcgcagcga agcgagtaat gtcatctgga ttttccttct
    12001 tacaggctat cgattaacaa tttgccttta cggaacatca tcagacgcag gaacaccacc
    12061 atcagcgcag ttatgactgc ccccagcgcg atccccgcca tatagacgcc caggttagtg
    12121 accagcggcc atgcccagat agcggattcc gggaaccatt gcactgcgcc cagccagaca
    12181 gcggcggttg agccgacaat cgcgccgacc atatacgacg gaatggcggt gatggggctt
    12241 tccagcgcaa acggaatcgc accctcacta atccccataa aggcaaggaa catcgcggtc
    12301 ttaccctgcg gataaagttg agcgttgaac aggcctttgc cggttaaacg acggtcaata
    12361 atggtcgcca gacccagacc aatcggagga ataacgatag cgatagaacg agcggtgacc
    12421 ggtaatacgt ggtcagtggt aaagctgaag gcaacgaaac ctgcggcttt gttgatcggg
    12481 ccaccaaggt cgattgctgt cgccgcggcg atccccattg cgtacatcag cgcgcccttc
    12541 tcacctgcgg cagtcagcac agtacggata ccgccgttga tccagccgcc aaacggggtg
    12601 atgacgtagt acatcgccag catgacaaaa atggcggaaa gaatcggtag caaaaatgtg
    12661 gttttgaacg ctaacaggaa atccggtaac tggattttct ggttcatcca tttcacaagg
    12721 taaccggcaa cgatagaaat aatcagcgcg ccaatgaagg tggacggcac cggcgaagag
    12781 gtcgcccatt gcatagtgct gggatcgaag ttcagcagtt gcgtcggctg ggtagacatc
    12841 aagccgccga taaaaccagc cgggaacgcc agtttgccac cgatagagtt cgccacaaaa
    12901 gcagcaaaca tcggaatggc aaaaccaaac aacacgccgc cgaaggactg cgacagccag
    12961 gcaaatttca gtaaggagag gtcgaaaccg gagaatttcc cgctattaag agcatccatg
    13021 atgccgatct cagcaggaat tttaagccag ctataagcaa tcagctggct gaaagcgagg
    13081 atcacaccgc ccataattaa ggtcggcacc atgcgtgaaa tccctgacat cacatgctgc
    13141 gggagttcac cccagaagct gcttttagag gcctgcggat tcttaactgc cgctgccgca
    13201 ccggatgcgc caggcacaac ggttgcactg cgttttttaa tggccataac aattatccct
    13261 gtcgaatatt actgttgttc agaggcaatc atctcttcga tttctttgat gatgcccgca
    13321 gcgtttttaa ttgcgtcctg caaagtgatt tcataaacgt cgcgtgattc gaaacgctca
    13381 ttatcttccg gggtaactgc cacggagtgg atgatgatgg ttgcttcggc gatatcctgc
    13441 gccgtcaggc gattctggat accgtccgcg ccctgggttt caattttcac ttcataaccg
    13501 gcttctaccg ccgcttcttc cagcgcctga gcggccataa aagtgtgagc caggcccatt
    13561 gggcaggcac ataaggcaat tagtttctta ctcatcgtta attcctcatt cagtgatagt
    13621 tgacgagtga aattatgaac gtgcaaaaca aatcgccgtt accagacaaa taatgcattt
    13681 actggagaat taatcccgct gtacggaagt gtgacgcagg tcggtcttgt gaggggacac
    13741 aaagaaaaat tgaattaact gtatgattta aaagattatc gggagagtta cctcccgata
    13801 taacaggaag gatttacaga atgtgaccta aggtctggcg taaatgtgca ccggaaccga
    13861 gaagacccgg attgtcatgg acgatgagat acaccggaat atcatagaca tattctttaa
    13921 agcgcccttt atcttcaaat gcggcacgga aaccagaggc tttgaagaac tcaaggaagc
    13981 gcggcacgat accgcccgca ataaacacgc cgccaaatgt cccgagattg agcgccagat
    14041 tgccgccaaa acggcccata atgacgcaaa acagcgacaa cgcgcggcgg caatcggtgc
    14101 agctgtcagc cagcgcacgt tcggtaatat cttttggctt gagattttct ggcaggcggt
    14161 tgtcagcttt cacaattgcg cgatacaaat tcaccagccc agggccagaa agcacgcgtt
    14221 ccgccgaaac atggccaatt tccgcacgca gtatttcgag gataatggcc tcttcttcac
    14281 tattcggcgc aaaatcaacg tgaccgcctt cgcccggcaa gcttacccaa cgcttatcga
    14341 catggaccag atgcgcaacc ccaagccctg ttccggcacc gtaaaccgca ataggtttac
    14401 cttcgaccgg ttctgcgcca ccaaactgaa tcagatgctc ttttttcagc atcgggatcg
    14461 ccatcgatac agcggtaaaa tcgttaataa tttccagatg gctaaaaccg agattctttt
    14521 tcatttcggc aattgagaac gcccaggtat ggttggtcat tgccacccag tcaccggtaa
    14581 ttgggcaagc gatggcaata cagccgtctt tcacctcgac cttatgttct tcaagataaa
    14641 cgcgaatgac cgcttcgagg ctggggtaat caagccctga ataggtctta gcctgcgaga
    14701 tttcaccact ggcaatatca cacagagcaa gacgtgcgtt ggtgccgccc acatcaccga
    14761 ctaatgcata ctttgtcatt cttcaactgc tccgctaaag tcaaaataat tctttctcac
    14821 actgtaaata cctgggggca taacaacaac gccggaaagg caggctccct gtaaatatcg
    14881 atctgggtca cacatttact ttatcgtttc agcaccaatt gcagcgatgc ctttttgcaa
    14941 gctgggcaaa ctaagtatct gaccccgcat aaggaataga acatgctcca tccgcgagcc
    15001 agaaccatgt tgttattatc gctccccgcc gtggcaattg ggattgcgtc cagtcttatt
    15061 ctgattgtgg tgatgaaaat cgcctcggta ttacagaatt tgctctggca acgactgccg
    15121 ggaactctgg ggatagccca ggattcacct ctctggatca tcggtgtatt aacgctaacg
    15181 ggtattgcgg tggggttggt tatccgtttc agccagggtc atgccggacc agaccccgcc
    15241 tgtgaaccgc tgatcggcgc accagttccg ccctctgcgc taccaggact tatcgtagca
    15301 ttaattctcg gtcttgctgg cggcgtcagc ctggggccgg aacatccgat catgaccgtc
    15361 aatatcgccc ttgcggttgc cattggcgct cgtctgttac cgcgcgtcaa ccgaatggag
    15421 tggactattt tagcctctgc cggaaccatc ggcgcgctgt ttggtacacc tgttgcggcg
    15481 gcgttgatat tttcgcaaac cttaaatggc agtagtgaag ttccgctatg ggatcgtctt
    15541 tttgcgccat taatggcggc agcagctggt gcacttacta ccggattatt tttccatccc
    15601 catttttcac tgcccattgc gcattacgga cagatggaaa tgaccgatat tctcagcggt
    15661 gcaattgtcg cggcgattgc catcgcagca ggaatggtcg ccgtatggtg cttaccacgg
    15721 ttgcacgcga tgatgcatca aatgaaaaat ccggtgctcg tgctaggtat tggcggattt
    15781 attctcggta ttctgggagt tattggtggg ccggtttcgc tgtttaaagg gctggatgag
    15841 atgcagcaga tggtggcaaa tcaggctttc agcaccagcg attacttttt gctggcggta
    15901 attaaacttg ccgccctggt cgttgctgcc gccagtggct ttcgcggtgg gcgaatcttc
    15961 ccggcagtgt ttgtcggcgt ggcattaggg ttgatgctgc atgagcacgt tcccgccgtt
    16021 ccagcggcaa taaccgtttc ctgcgctatt ctcggcatcg tgctggtggt aacacgcgat
    16081 ggctggttaa gtctttttat ggcggcagtc gttgtaccca ataccacatt gctaccgctg
    16141 ctctgtatcg tcatgcttcc ggcatggctg ttattagcag gtaagccgat gatgatggtc
    16201 aatcgtccga agcaacagcc accccacgat aatgtttagc aaaaaatgct cttctttatg
    16261 atttaagagt tatggcgatg atacggggct ttacctcccc gtaatattgc gttaacaggc
    16321 cgctgacggc ttcgatcgtg aaggagaata acaatgttca ggtcactgtt tctggcggcc
    16381 gccctgatgg catttacccc gcttgcagca aatgcaggtg aaatcaccct actgccatcg
    16441 atcaaattac aaattggcga tcgcgatcat tacggtaatt actgggacgg tggtcactgg
    16501 cgagatcgtg attactggca tcgtaattat gaatggcgca aaaaccgctg gtggcgtcat
    16561 gataatggct atcatcgtgg ctgggataag cgtaaagcat atgagcgtgg ctatcgtgaa
    16621 ggctggcgcg atcgtgacga tcatcgcgga aaaggccgcg gacatgggca ccgccattaa
    16681 acgcgtcctc caatggagca caatgcctga tgcgacgcta gcgcgtctta tcaggcctac
    16741 aaaccggctc tttaacgcgt agggcggata aggcgtttac gccgcatccg ccaatagtgc
    16801 cagatgcgac gctcattcaa ctacaatccc agcgccgtcc ccaccaacaa ccagatattt
    16861 agcgccacga ccagcactac aatcacccag cctgtctgtt ttacgcgttt gctgttcacc
    16921 agatcgccca tcaacttgct gtcactggtg aaaatcagca gtggaaccag cgccagggcg
    16981 ataccaaaac ttaacagcac ctgactcata accagaatcc gtgtcggatc taatcccatc
    17041 agaatgacaa taaatgacgg cagcatggtg actgtacgac gcacccacag cgggatatgg
    17101 aagcgaatga acccctgcat caccacctgc cctgccagtg tccccaccac cgttgaggac
    17161 agtccggcag caaccaggct taatccaaag accgttgccg cagcatggct taacaacggt
    17221 tgcagcgtca gataagcctc atcaagatcg gcaacaccag tatgaccaga aaagtggaac
    17281 gccgccgcag ctgtagccat catcgccaga ttgacaaaac cggcaatcgt catggcgata
    17341 gccacatccc atttggtggc ggaataacgt tgttgacgcg aaccgccatg taaatgctga
    17401 gtgagcgaag agtgcaaata aatcacatgc ggcataatcg tcgcccctaa cacgcctgct
    17461 gccaggaaga ccgcctccga agtaggtaaa ctcgggatca ccattccttt acccagctgc
    17521 gccaggttag gctgggagaa aatcaactcg acaatgtaag ccgcggcaac aaacaacagt
    17581 aacccgccaa tcactttctc cagcggtttt tgcccgcgac gttgcagcat taaaatcagg
    17641 aaagtcgcga tccccgtcag caccgcgccc tgcaacaacg aaacaccaag aatgagttta
    17701 aaaccgatcg ccgcaccaat aaattccgcc aggtcggttg ccatcgcaat aatttctgcc
    17761 tgaacccaat agaaccacac tacgggacgc ggatagtgat cacgaatctg ctccgccaga
    17821 tttttaccgg tggcaatccc tagtttggca gagaggatct gaatcagcat cgccatcagg
    17881 ttggcccaaa cgacaaccca cagtagctga tagccgaagc tagcacccgc ctgaatattg
    17941 gtcgcaaagt taccgggatc gatataacca atcgccgcaa tgaacgcagg tcccattaat
    18001 gcgagcctca tcttgcgcgc cgcccgtccg ctgctactct caacgcgata gttcgtcatc
    18061 ttgtgcctct aaaacatagc ctttgctatg tttcatgcta tgccaaacga gaatgattat
    18121 caaattcatt tgaatgggtt gtggtgattt ctttgataga ccaggattat gactacgaaa
    18181 agattgatga cttgaatgtt atggttatgt ttaatgttag cacatttaca taactttcag
    18241 cttccataca caacatagca gaaatgtatg acagatcact atttttgaag cttgtcacag
    18301 gacgtcatta tagtgtgtgt cagatctcgt tttccttaac catgttacat agaatgtgca
    18361 cggaaattta acctgcctca tatttggagc aaatatggac cgcgtccttc attttgtact
    18421 ggcacttgcc gttgttgcga ttctcgcact gctggtaagc agcgaccgca aaaaaattcg
    18481 tatccgttat gttattcaac tgcttgttat cgaagtgtta ctggcgtggt tcttcctgaa
    18541 ctccgacgtt ggtttaggct tcgtgaaagg cttctccgaa atgttcgaaa aactgctcgg
    18601 atttgccaac gaagggacta acttcgtctt tggtagcatg aatgatcaag gcctggcatt
    18661 cttcttcctg aaagtgctgt gcccaatcgt ctttatctct gcactgatcg gtattctcca
    18721 gcacattcgc gtgttgccgg tgatcatccg cgcaattggt ttcctgctct ccaaagtcaa
    18781 cggcatgggc aaactggaat cctttaacgc cgtcagctcc ctgattctgg gtcagtctga
    18841 aaactttatt gcctataaag atatcctcgg caaaatctcc cgtaatcgta tgtacaccat
    18901 ggctgccacg gcaatgtcca ccgtgtcgat gtccatcgtt ggtgcataca tgaccatgct
    18961 ggaaccgaaa tacgtcgttg ctgcgctggt actgaacatg ttcagcacct ttatcgtgct
    19021 gtcgctgatc aatccttacc gtgttgatgc cagtgaagaa aacatccaga tgtccaacct
    19081 gcacgaaggt cagagcttct tcgaaatgct gggtgaatac attctggcag gtttcaaagt
    19141 tgccattatc gttgccgcga tgctgatcgg ctttatcgcc ctgatcgccg cgctgaacgc
    19201 actgtttgcc accgttactg gctggtttgg ctacagcatc tccttccagg gcatcctggg
    19261 ctacatcttc tatccgattg catgggtgat gggtgttcct tccagtgaag cactgcaagt
    19321 gggcagtatc atggcgacca aactggtttc caacgagttc gttgcgatga tggatctgca
    19381 gaaaattgct tccacgctct ctccgcgtgc tgaaggcatc atctctgtgt tcctggtttc
    19441 cttcgctaac ttctcttcaa tcgggattat cgcaggtgca gttaaaggcc tgaatgaaga
    19501 gcaaggtaac gtggtttctc gcttcggtct gaagctggtt tacggctcta ccctggtgag
    19561 tgtgctgtct gcgtcaatcg cagcactggt gctgtaagac catacataaa aaagccgggg
    19621 ataattcccc ggttttttta cgcctgttaa tcagctaatg gctgcgggcg accaatcaaa
    19681 tacccttgca gatattgcac cccgagctta tgcaatagcg cctgctgctg ttgcgtctcg
    19741 acaaactccg cgaccacact caatgacttc gctttcgcca gatcggtaat tgatctcaca
    19801 atcatcgcat ccagcgtatt cgtgacaata tctttcacaa agacgccatc aattttgatg
    19861 atatcagcct gcaaacgctt taaccgttcg tagttggcat atccggtgcc aaagtcatca
    19921 atcgcaatcc ggaaaccaaa cttatgcagc tgctcgatgt tgtacatgct gctttctgcg
    19981 ttagaaaacg cctgctcctc ggtgatctca agaatgaccg cctgcgggga gatgtgataa
    20041 cgcttaaaca gacgaataat ccgcccggca atattctttt gcagcagcgt gagcggcatt
    20101 aaattgactg aaaagcgcgg tccttttttg tcgcaagggt gtgttgccag ccacttcaac
    20161 aaggattcca gcacttgcaa atcaaaacgc gcgctaaggt taaactgagc aataaggggc
    20221 agaaacttat ccggggtcat aatgccgccg tcatatttca gtcgcgcgag gatctcatca
    20281 taaccgtcac cctctttgtt gcgaattggc tgggcgtaga gcagcaaatc accctgatct
    20341 aacgccgtgc gaatggtatt cagcaatagc acctgtttag tggtctgccc tgaaaccatc
    20401 tcctctctgc tatccagcgc cagcacatga tgatgtgcgc aggattgctc cgccagccag
    20461 cttaactgcc ccaacaaggg ttgcagggtt tcctgatttc catcaaaacg cccccaggca
    20521 gcaccatagc ccatatccag cccggtattg ttccagtgaa tttgccgact attcaggatg
    20581 ttaaccatat gctggagtcg cccttccgtt tccggcccgc tcagcactaa cagcagttca
    20641 ctacccggca attgatacaa cttttcgttt tcctgcatca acggcagcag cgtacggcaa
    20701 attgagcgga tacagtgaac gcgcatcatt aagccgtaat gacgactcat aaactcaaga
    20761 ttatcaatgc gcaggcagca aaaactcttg cccgcctctt gctccggcgc ttgttccaac
    20821 gcacgaaagt tgggtaggag cgttaacgga tctgtcagcg cctgcaaatg ccagcggcga
    20881 ttaagccatt cactgcgatg ataaatgcgc accatataga gcaggcaaac gctaaaggaa
    20941 atcagcaccg caagaataaa tgccagcgaa tattcggttt ccaccccttg caaaaagttc
    21001 tggttgtaat tcagcaggca aagcgttgaa acagcccagg tgagatttaa aaacggatag
    21061 cgaagcttac cgaccccgag ggtaaagatg atgaagaaaa cgggtaccaa gtaaccggca
    21121 ataaagtcgt tttcataagg tgtgcacaac agaagtagca gcacgcttaa cgctgccagc
    21181 cagcttaagg taaatgcgcg tttctctttg cccaacgacg gagcgatatc cctgcgccac
    21241 aatatctgcg caaagtgggg acttacaatc atgcgagtga gatagtagaa aagcatgttg
    21301 taaatcagca cagcggtgaa aaggcttagc aaatcaacga ccgtgaaaat ggcatccgca
    21361 tcgccgaaaa aggtggatat ctttagcggg aaatcaaaga agctccccac aagatacatg
    21421 ctgcatttga tgccgatcgg cgtcaccaaa ccaagccaga acagacgttg ccagacatat
    21481 cggctggtca atccataacg ccaacgcgtc cccagctgcc agcgtaatat tgcgcaggca
    21541 cacaacacga caaacgtctg gctaccgagc atgaccgcag tctgtaaaaa agagagatga
    21601 aagttccaca gattggtgca aaacatcccg cataaaacgg gaacgacacc gcgccagcca
    21661 aagataaaca agattgacag cattacgcac agtggcatcc acgccagaaa gatataactg
    21721 gaatggataa tggcaagtgg cgaaataaaa cgggatagct gaataagtac cacggtgagc
    21781 gtaaacgcta gtgtgaatat cttgatattt tttatcaggt tatgctccac aaacatgagt
    21841 acactactct cactgatcgg ggttttcagg cacacaaata ataagtttat tcgcatcgcg
    21901 aagcaaacag gggccagcct gttgccgatt tttcatccaa tgatgctttc accaggcggc
    21961 gctaacccgg acgtagactg tattacaaaa gcggcaaaaa gcagagacaa aaaacccccg
    22021 ctttgcagcg agggttggaa atttggtgga gctaagcggg atcgaaccgc tgacctcttg
    22081 catgccatgc aagcgctctc ccagctgagc tatagcccca cgatgcgttt acgtaccaaa
    22141 tttgctgggt gcaaaatttg gtggagctaa gcgggatcga accgctgacc tcttgcatgc
    22201 catgcaagcg ctctcccagc tgagctatag ccccgtcacg taaagcttgt cgagttgacg
    22261 ggcggcatca tatgaattcc gcccgaatgt gtcaacggca aattgcaacg tgtagtttca
    22321 atcgctgaaa aatcaggcaa atgaacaatt ttggaaaacc gctcgcattc agtagttatt
    22381 catgtcacgg tttcctgtaa agtggtgtta taaaatgaac tactaataga cccacataca
    22441 ttcagggaat tgttatgttc aaggagagga tgacaccaga tgaacttgcc agactgaccg
    22501 gctatagccg ccagaccatt aataaatggg tacgcaagga aggctggaca acgtcaccaa
    22561 aacctggcgt ccagggtggc aaagcccgac tggttcacgt caatgaacag gttcgtgaat
    22621 atattcgcaa tgctgaacgt ccagaaggtc agggagaagc gcctgccctt tctggtgatg
    22681 cgccgcttga agtattgctg gtgacactgg cgaaagagat gacgccagtt gaacaaaaac
    22741 agtttacatc cttgcttctg cgggaaggga ttatcggatt gttacaacgc ttagggattc
    22801 gcgatagcaa ataatatgaa aagattacgc aacaaaatga ccaccgaaga actggctgaa
    22861 tgcctcggtg tggctaaaca aactgttaac cgttggatca gagaaaaagg ctggaaaacg
    22921 gaaaaatttc ccggcgtgaa aggcggtcgt gccagactta ttctggtcga tacgcaagtt
    22981 tgcgagttta ttcagaacac gccagccttc cataacacac caatgttgat ggaagctgaa
    23041 gagagaattg ctgaatacgc tcctggagca cgcgccccgg cgtatcgaca aatcatcaac
    23101 gcgatagata atatgacaga cattgagcag gaaaaagtcg cgcaattttt gtcacgtgaa
    23161 ggaattcgca atttcctcgc ccgtctcgat attgacgaat cagcataaat aaaaaacggc
    23221 aggatattat ctcctgccgt ttatcttttt acacgctaat tactgctgat tttcgcgttc
    23281 agcaataaaa tccagcgctt tgttgatacg ctcgatactg cgggtcttac caattgcgtg
    23341 aacggtaaca tccagtgctg gagactgccc cgcaccggtt acggcgacac gcagcggcat
    23401 accaacttta cccataccca cttccagctc atccgccgtc gcctgaatag cgtgatgaac
    23461 gttttcagcg gtccagtcag taatcgcggc cagtttgtca cgaaccactt ccagcggctg
    23521 acgcgctacc ggacgcagat gttttttcgc cgcatcggca tcgaactcag caaaatcttc
    23581 gtagaaataa cggcagctct gtgccatctc tttcagcgtc ttgcagcgtt cgcccagcag
    23641 tttcaccaga tcagccagct gcgggccgtt acgggtatcg atattttcct gctcaatgtg
    23701 ccactgtaag tgagtagcaa catactccgg cggcagcgcg ttaatgtagt gatggttcag
    23761 ccacagcagc ttgtcggtgt tgaacgcact ggcagatttg ctgacggcat tcaaagtgaa
    23821 gtatttgatc atctcttcac gagtgaagat ttcctgatcg ccgtgggacc agcccagacg
    23881 caccagatag ttcagcagtg cttctggcaa ataaccgtca tcacgatact gcattacgct
    23941 gactgccccg tgacgtttgg acagtttttt accgtcatcg ccattgatca tagaaacgtg
    24001 cgcgtaaacc ggcaccggcg ctttcagggc cttaagaatg ttgatctggc gcggcgtgtt
    24061 gttgatatgg tcttcgccac ggataacgtg ggtgatttcc atatcccagt catcgacaac
    24121 cacacagaag ttataggttg gggaaccatc ggtacggcgg ataatcagat catccagctc
    24181 ctggttgctg aactcgatcg gaccacggat ctgatcgtca aaaacaacag aaccttcctg
    24241 cggattagca aaacgcacga cacacggttc atcatcagca tgatgctcat ggctgtggcg
    24301 gcagcgaccg tcataacgcg gcttctcacc tttcgccatt tgctcttcgc gcagcgcttc
    24361 caggcgctct ttagagcaat agcatttata agcagtgccc tcttccagca tctgatcgat
    24421 caccgcattg tagcgatcaa aacgtttggt ctggtagtac ggaccttcat cccactccag
    24481 gctcagccag ttcatgccat ccataatggc ttcgatagct tccggcgtgg aacgctcaag
    24541 atcggtgtct tcaatacgca gcacgaactc accgccgtgg ttacgtgcaa aaagccagga
    24601 gtaaagagca gtacgcgcgc cgccaacgtg cagatagcct gttgggcttg gcgcgaagcg
    24661 agttttgatt ttcatgaaat ggccttacgt ttagaaagat gccgacaacc ggcaaatcct
    24721 ggaaaaattg agtgggcgat attctatcac tcccgcctga ttcctcaatg tagttcgggt
    24781 tgcaacctta cgcattattg ttttcgttga caaattgcgc aacacggtct gttttgcgat
    24841 catcttgctt aaatttacga cgaacgaaca atttctttag aaaatgcgtt gactcatttt
    24901 gaactctccc tataatgcga ttccacacag cgggggtgat tagctcagct gggagagcac
    24961 ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccaa ctactttatg
    25021 tagtctccgc cgtgtagcaa gaaattgaga agtgggtgat tagctcagct gggagagcac
    25081 ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccac tttctcgcca
    25141 gctaaatttc ttgtaaaaat gtgaagtacc gaagtgggtg attagctcag ctgggagagc
    25201 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc acttcgggtc
    25261 gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc
    25321 acgacccacc aatgtaaaaa aagcgcccta aaggcgcttt tttgctatct gcgatactca
    25381 aagattcgaa gctgccgcag gttcgagttg agcgcagcga aacaacggag ccgctcgcgg
    25441 cgacggcccg aagggcgagc gaagcgagtc atcctgcacg acccaccaat gtaaaaaagc
    25501 gccctaaagg cgcttttttg ctatctgcga tttgcgaaat tgcctgatgc gcttcactta
    25561 gcagacgact atttccggca attcctgtct cctcacctac tgtgtcaatg cagccaacag
    25621 cttaaccatc gcgggcgtca cctgctgtgt ttcataaaca atatataaat ctgcagggat
    25681 gcgctgtttg agcggacgga aaatgacacc tggccagttc atttgtgcgt agctgtccgc
    25741 tatcaatgtg ataccaatac ccatactgac catggcgagt accgtttgcg gttcattaac
    25801 ttcgcgaata acaaccggtg aaaatcccac ctgctggcaa actcgctgca aaaaatccca
    25861 gtcagtgtac acgggcggca ttgtaacaaa atactcgtca cgtagcgctt ccagcggaac
    25921 ggtggaaaat gatgagagat gatgctcttc aggcatcgcc accagaaacg ccgattcatg
    25981 caaccgtaag ctggtaaaac ctgtcggtgg ttctgtcgcc attcgccaga tcccggcatc
    26041 gagttcgcgt cgttccagca gggccatttg catcgcgggc atcttttcgc gaaaaagaac
    26101 ttcaacgtta ggattttccc tgaggaatcg ccgcataacc gggcgcatcc gtccccacat
    26161 agccgttccc actacgccga gttcaatccg ccctgcttct ccccgaccta tttgttcaac
    26221 ccgagccaat acattattag cattcaccag caatcgacgc gattcttcca tcaagatttt
    26281 gcccgcgtgt gtcagtacga cgctgcgcga atggcgaata aaaagctgcg tgccgagttg
    26341 attttccagc tctttaatat gaatactgag cggaggctga gacatattta aacgcgctgc
    26401 tgcgcggcca aaatgcaact cttccgctac ggcaagaaaa taacggagca acttaagatc
    26461 tgttctgtat acgcgttcca taattaagtg ctccatataa taaatacatc gtattaaatt
    26521 atcatggcat aaagtatttc accaccagat atccgacata cataaacaca atggataata
    26581 tacctggtaa tacatttggt acaggaatag gcaagcgcac gattgtaaac aaagcaccaa
    26641 tgccaaaacc aacgccggaa gaaaataaaa tatctttcac taattaacct ttatcataaa
    26701 agcagctctg aagagcagag ctgcgaatcc ttttaatgag ccaccgctcg atgctttatc
    26761 ttttcagggt catgattata tttaaaccca aagaaaaata tcactgcgag aaaaagagca
    26821 tatcctgcaa acaccagcca gatagtttgc cagtctttta cgccatccac cgaaaagtaa
    26881 tctactgcca tgccactcag aatcgagcca acccatgcgc cgacaccatt taccatggtc
    26941 ataaagagcc cctgcgcgct ggcacgaatg ctggaatcaa cttcctgttc gacaaatacc
    27001 gaaccagaaa tattgaagaa atcgaatgca cagccataaa caatcatcga cagcagcagc
    27061 aaaataaatc cggttgttga cggatcgcca taggcgaaga agccaaagcg cagcgtccag
    27121 gccaccatac tcatcagcat gacggtttta atgccaaatc gctttaaaaa gaatgggata
    27181 gtcagtataa agcccacttc tgccatctgt gaaactgaca gtaaaatgga gggatatttc
    27241 accacaaaac tgtcagcaaa ctccgggtta cgggcgaaat catgtaggaa cggattacca
    27301 aaaacgttgg taatttgcag taccgcaccc agcatcatgg caaagaggaa aaagatggcc
    27361 atgcgtggat ttttaaacag cacgaaggca tccagaccca gcttgctggc aagcgatgtg
    27421 gtcgcttttt tctccgcaac cggaatcttc ggcaaagtca gcgcataagc cgacagcagc
    27481 aatgacgcac cggacgcgat atacagctgc agactactca attccagatg cagcaggctt
    27541 actgcccaca tcgcgacaat gaaccccacc gtaccaaaaa cgcgaatggg cgggaaagcg
    27601 gtcaccgggt caagccctgc ctgggcaaga caggaataag agacgctgtt cgataacgca
    27661 atagtcggca taaacgccat cgcattgact aacatcaccc aaaacatcat atccggatca
    27721 gttacggatg ccgcataaaa aagtacgccc gcacacacca ggtgacacag catgtatgca
    27781 cgttctgcgc gcagccattt gtctgcgatg atccccatta taccaggcat aataatcgcg
    27841 gcgatccctt tggaactgta aaccatgcca acattagcgc cggtgaaatg aagggtatta
    27901 atcatgtaag agccgagggt aaccagccag ctcccccaga taaaatattg caaaaaggac
    27961 attaccttta agcgcatcgc gatgctcata atcgtttcct tgtcgcatca tttggcctca
    28021 cccgcgaggc cgtttcttat tgtttttctc aggcaatttt gcgcagaaag ccacaaataa
    28081 ggttgatgaa gttctgctta gagagctccg cagccgccag cgtttgcgca tgtgacaatt
    28141 tcacatcgct taaaccttcc gccatattgg taatcgcaga gaccgcaacg actttaaggt
    28201 cgcaatggcg agctgaaata acctcaggca ccacagacat accaacaaca tccccaccaa
    28261 taatttgcat catgcgaatt tccgccgcag tctcgaaatt cggccccgga tacgagacga
    28321 acacgccctc cgtcagaggg aaaccctctt ctttcgccac tttttgtaac agtgcgcggt
    28381 attccgcatc gtaggcattt gccagtgaga agaagcgctc tccaaaacga tcatcgttaa
    28441 gacccaccat cggcgtaccg ggcatggtgt tgatatgatc tttcaatgcg accaggctgc
    28501 ctgcccccac ttccggacgc agcgagcctg ccgcattggt gcagaacagt agctcgcagc
    28561 ccagcagctt aaaggtacga attgcgtcag tcataatggt cattccacga ccttcgtaga
    28621 aatgtccgcg acctttcata cataccaccg gtacgccctg gagatggccc agcactaact
    28681 cccccgcatg accatgtacc gtacttaccg ggaagcccgg cagcttttcg taggaaattg
    28741 cgacagcgtt ctcaatctga tcggccagcg cgccgagccc ggaacctaaa ataaaggcca
    28801 ctcgtggcgt gaaatcaggt ttataagtct tgataatatc tatgcaaaac agcgggttat
    28861 gagaaaattg aacctgagac atacatatcc tttttctgta gggtggaatc taacgccttt
    28921 ttatagcgag gttgtcccgg ctcttaccaa tacacttttc tcacgcattc aatagcgatt
    28981 cgatatcgat ggatttatat cttttctcta ttaatccacg gtaaaaactg tattggcgat
    29041 attaattcct ttgcattaac aatatgtcgt ttttagcaaa ccactttatt aagcaatcta
    29101 ctaataacga tcggcatagg gaattatgct atgaaaaaac atcttttaac tctgacactt
    29161 tcctctatat tagcgatacc tgttgtatct catgcagaat ttaaaggcgg ttttgcagat
    29221 atcggcgtgc attatctgga ctggaccagc cggactaccg aaaaatcgtc gaccaaatca
    29281 cacaaagatg attttggtta tcttgaattt gaaggtggcg ctaactttag ttggggagaa
    29341 atgtacgggt tcttcgactg ggaaaacttc tataacggtc gccataataa accaggtagc
    29401 gagcagcgtt atacctttaa aaataccaac cgtatttacc tgggtgacac cggatttaat
    29461 ctctacttgc atgcgtatgg cacctacggt tctgcgaatc gcgtgaattt ccacgacgat
    29521 atgttcctgt acggtatcgg ctacaatttc accggcagcg gttggtggtt caaaccgttc
    29581 tttgctaagc gttatacaga tcaaacctat tacactggcg acaacggcta tgtcgccggc
    29641 tgggttgcag gttacaactt tatgctgggc agcgagaaat tcactcttac caactggaac
    29701 gagtacgagt ttgaccgtga cgctacctat gcagcgggta atggcggtaa agaagggctg
    29761 aatggcgcag ttgcactgtg gtggaatgca acatcacaca ttactacggg gattcagtat
    29821 cgatatgcgg atgacaaact gggcgaagat ttttaccagg atgcgataat ctactccatc
    29881 aaatttaact tctgattgat tactgccggg gctcccggca gtatgaattc actctatatc
    29941 tgatacaacg gatccccttc ccgaccagcc attagcgcat tgccctggtc tcgtaccacg
    30001 tcccataacg cttcagcggc ggtggaaagt gaacggttct tcctgcgcac cagcatcaac
    30061 tggcgttcaa caaccggcgt aatgcgcttt accaccagtg ggctaccttc aggcaatggt
    30121 agcgccagtg cgggaagaat actgatgccg atgcctgcgg ctaccatcgg gaacagcgtc
    30181 gccggatgtc caatctcctg cacaatattc gcctgaatac cattgcgcgc cagcgctgcg
    30241 tcaatcagcg gtcggctgcc tgacgcgtaa tcctgcaaca ccaatttcgc tccctgtaat
    30301 gcctgccagg gtacataatc ttcgacggcc aaagcgctat cgcgatggca aagcagaaag
    30361 aaaggctcgg aaagaatcgc ttcacattgc agatcgccca ctgggcctgg atcgatgacg
    30421 atgccaaagt cgacatctcc ctggcgaata ctttccatca cccactgctg cggacgatcg
    30481 tgcaggacaa actgaatatc tggatagcgg cgatggcttt cagcaatgca ttgcggaata
    30541 agatgcgcgg aaatggtctg gctggcagcg acccgaactt ttccgctcag ttgttgcccc
    30601 atacgcccgg tatcgcgcaa cgtgctgttc agttcatcca gcagtcgctc aagacgcaaa
    30661 gccagctgct gccctgcatc tgtaagcacc acttcacgcg tggttctgtc cagcaggcga
    30721 acaccagtat gattttccag ttccttcaca ctgtgactca ctgccgactg gctcaggccg
    30781 atacgctctc ctgcacgact aaaacttttc tcctgcgcta ctgtgacgaa aacctttaat
    30841 tgttttaaag aataattcat ctgttttctt catgaatgga tgcaataaat caattttatt
    30901 tctcaaactg gaagaagcac aatagaacca tcgatcatct ggagtcttta tgaaactttt
    30961 tcgtatcctc gatcctttca ccttaaccct gatcacggtg gtgttgctgg cctctttctt
    31021 tccggccaga ggcgatttcg tccccttctt tgaaaatctg accaccgcag cgattgccct
    31081 gctgttcttt atgcacggcg cgaagttgtc gcgtgaggcg attattgctg gcggtggtca
    31141 ctggcgactg catttgtggg taatttgcag caccttcgtg ctgtttccga ttctgggtgt
    31201 actgtttgcc tggtggaaac cggtaaatgt cgacccgatg ctctactccg gttttctcta
    31261 cttgtgcatt ctcccggcta ccgtgcagtc tgcaatcgcc ttcacgtcaa tggcgggcgg
    31321 taacgtcgcg gcggcggttt gttctgcgtc agcatccagc ctgctgggga ttttcctttc
    31381 accattgctg gttggtctgg tgatgaatgt tcacggtgca gggggcagcc ttgagcaggt
    31441 cggtaaaatt atgctgcaac tgctgctgcc gtttgtgttg gggcatcttt cccggccgtg
    31501 gattggtgac tgggtgtcgc gcaataaaaa atggattgcg aaaactgacc agacgtccat
    31561 tctgttggtg gtttatacag cattcagcga agccgtcgtt aatggtatct ggcataaagt
    31621 tggctgggga tcattgctgt ttatcgtggt ggtcagctgc gttcttctgg ctatcgtgat
    31681 tgtagttaac gtctttatgg cacgccgact gggcttcaat aaggcagatg aaattactat
    31741 cgtcttttgt ggttcgaaaa agagtctggc aaatggcatc ccgatggcaa acattctgtt
    31801 ccccacatcg gtgatcggta tgatggtgct gcccctaatg attttccatc agatccaatt
    31861 gatggtctgt gcggtgctgg cgcgtcgata caaacgccag accgaacagt tacaggcaca
    31921 gcaggaaagc agcgccgata aagcttaaag cggacgcttc aggggctgga ccagttgcgt
    31981 cagcccctcg gttttaatca gcagcgtgat ttgcattagc tcaccgagct tccctgccgg
    32041 gaattcatct ttgcgggcaa accacagcag atactcctcc ggcaggtcga ttaagcgacg
    32101 ccctttatat ttgccaaacg gcattatcgt attggctatt tcaatcagct gctctttttc
    32161 catctcagct acccagcaaa cgcagcattt ccgcttcgtc gatgacttca atgcccagtt
    32221 cctgcgcttt cgccagttta gaacctgccg cttctccggc aatcaccaga tcggttttct
    32281 tcgacacgct gcccgcgact ttcgcgccca gttcaaccag tcgagcttta gcgtcatcac
    32341 gcgacatctg gcttaagctg cccgtaagca ccacggtttt accagcaaac gggctgtcaa
    32401 tctcttccgc gttgataacg atcggcgcag gccagtgaac accttccgcc aacagctcgc
    32461 tgatgacatt gcggttgctt tcttcggcaa agaagttgtg aacgtgggat gcaacgacaa
    32521 tgccaacatc aggcaccttt tgcagctctt caatcgaagc ggcttccagc gcttccagcg
    32581 tgccgaaata tgccgccaga cctgctgcgg tggcctcgcc gacttcacgg atgccaagtg
    32641 catagaggaa gcgagcaaag gtggtttctt tcgctttttc cagcgcgtta accacgtttt
    32701 gtgccgattt tggccccata cgctccagtc cggtcagttt gcctgcggtg agtttgaaca
    32761 gatctgccgg agtatggaca tattcttttt caaccagctg atcgatgatt ttgtcgccca
    32821 ttccgtcaac atccatcgca cggcgggaaa caaagtgttt cagcgactct ttacgctgcg
    32881 caccgcaaat caggccaccg gtacagcggg caaccgcttc accttccaca cgctcaacgt
    32941 cagaaccaca taccggacaa tgcgtcggga atacaacctc acgggtatct tccgggcgtt
    33001 cagaaagcac gacgttaacc acctgcggga tcacgtcgcc agcgcggcga atcaccactt
    33061 tatcgccaat gcgtaaacca agacgttcga tttcatccgc attgtgtaag gttgcgttac
    33121 tcaccagcac gccagcaaca tggacaggtt ccagacgcgc aacaggcgta atcgcgccag
    33181 tacgcccaac ctgaaactcg acgtcacgca caaaggtcat ctgctcctgc gccgggaatt
    33241 taaacgctac cgcccagcgc ggggcacgcg cgacaaagcc aagctgctcc tgctgttcca
    33301 gtgagttgac cttaatcacc acgccgtcga tatcaaagcc cagcgtcggg cggtcttctt
    33361 ccactttgtg atagaacgcc agcacttctt ccgccgattc acaaagcgtt acccgatcgc
    33421 tgaccggcaa cccccacttt ttaaattgca gtaaacggcc aagatgagta tccggcagct
    33481 cgccaccttc cagaacacca acgccatagc agaaaaaagt gagcggtcgc ttcgctgtaa
    33541 tacgcggatc aagctgacgc agtgaaccag ctgccgcatt acgtgggtta gcaaaaactt
    33601 tcccgcccgt gcgtcgcgca tcttcgttaa tcttttcgaa ccccgcctgc ggcaggaaca
    33661 cttcaccacg cacttccaga cgcgccggga tattctctcc gtgcagcttc agcggaatag
    33721 cgcgaatagt acgcacatta gacgtgatat cttccccggt ggtgccatcg ccacgggtcg
    33781 cggcactgac taaaacgcca ttttcataca gaatactgac ggcaaggcca tccagcttca
    33841 gctcacagca ccaggtgact ttctcgttgt ttttcagacg gtcctgcaca cgtttgttga
    33901 aagcaagaaa gctttcttca tcaaaaacgt tatccagtga cagcattggt acttcatggc
    33961 gtatctggct gaaagccgcc agcggcgcag cgcctacacg ttgggtaggc gaatcaggcg
    34021 taatcagttc tggatgtttg gtttccagct cgcgcagttc gcgcatcagc ctgtcgtatt
    34081 cagcgtcggg aatttccggc gcatccatca catgataaag atattcatga tggcgaagcg
    34141 tcgttcgcag ttctgtcagt tgttgttcga ttgattccat atcgcaccat caatgctaaa
    34201 aacccccgac aagcgggggt tcgaagagga gttaatttgc cttaagtgta tcaggcgttg
    34261 gcgtctttga cttcgcggat gatgtcctgg tactcgcgca atttctgcgg agtcatcata
    34321 cggcgctgat cgtcaagcac gacaccgccc acttcatcgg caatatgctg cgcagattgc
    34381 agcatcagct tgaagttctg cagctcgtca ccgtaagacg gtacctgcat aaagatggtg
    34441 acacccggag tagtgaaatc cttcatttca ggatcaaagg ttcccggttt caccatattc
    34501 gccaggctga ataacgccgg gccgctgcca tccgggctaa gatgacgatg gtaaatattc
    34561 atatcgccaa aaatgaagcc cgcttgttga atgctgttaa gaagcagttc accgtttagc
    34621 tcgctaccgt gatgcgccgc gacgttcatg ataatcaccg cttctttgcg cttcggttta
    34681 tccataactg gagccggttc cgctacaggc tcaggctgtg gtgccgctac gggttctgca
    34741 ggctggaaag cctgttgtgc cggttgcggt gctgaatgca caggctgcgg cgctggcgcg
    34801 acctgtggcg aaactggctg ctgcaacggc tgttcaggct gcggctgata ggcaggctgc
    34861 tgcaccggct gcgctggacg cggagcatgt tgcggcggta cctgcgcttc aggcggctgc
    34921 tggaccggtt gacgcggctg cgcagacgca taaggcggtt ggtactggtg ttgcggcgac
    34981 ggacgagcag cctcatgctc ctgagcgtta gccggggcat gattcacgcg gtgaacacga
    35041 acctcaccaa cgccctcatc atcttcgaca tcctcgtcat aagaatcgtc gtcacgtttt
    35101 gacttcattc gttttaatgg ccgatcgcgg aacatagaag atcgttcttt acggctggtc
    35161 cagaaaccat gtaccagtaa agcgattatg gcgatcgcgc caacaatgat taatatcaga
    35221 cgcaaatcct gcatcattat attctctgtt gttctaacac cttgccacca cggcaaacat
    35281 ttactcacta agagtatttg ccgattacct caagtgcaag tgcactatta actttcacag
    35341 cacaaagata gatgaaatcg tgctttttgc tgttttttcg aacatatcct aactgtccat
    35401 tgcgcaatta cccggtaaaa tacgcagaat tttcctggga ttggtcaaaa ggagctcatc
    35461 ctgattatgg tttcatcatt cacatctgcc ccacgcagcg gtttttacta ttttgcgcaa
    35521 ggctggaagc tcgtctcgca acctgggatt cggcgtttcg ttattttacc gctgctggtc
    35581 aatattttgt tgatgggggg cgcattctgg tggctcttta cacagctcga tgtctggatc
    35641 ccgactctca tgagttacgt tccggactgg ctacaatggc tgagttatct gttgtggcct
    35701 ctggctgtca tctctgtgct gttagtgttt ggctatttct tctccacgat tgctaactgg
    35761 attgccgctc cgtttaacgg tttattggct gaacaactgg aagcacgatt gactggcgct
    35821 acaccgccag ataccgggat tttcggtatc atgaaagatg tgccgcgaat catgaaacgc
    35881 gaatggcaaa aatttgcctg gtatctgccg cgcgcaattg tattgctaat tctttactta
    35941 atccctggta ttgggcaaac cgtcgcgccg gtactgtggt tcctgtttag cgcctggatg
    36001 ttagccatcc agtattgcga ttaccccttc gataaccaca aagtgccgtt taaagagatg
    36061 cgcaccgccc tgcgcacacg caaaatcacc aatatgcagt ttggtgcttt aaccagcctg
    36121 tttacgatga tcccgctgct taatctgttc atcatgcccg ttgccgtttg tggcgcgacg
    36181 gcgatgtggg tcgattgcta tcgcgataaa cacgcgatgt ggcggtaaca atctaccggt
    36241 tattttgtaa accgtttgtg tgaaacaggg gtggcttatg ccgcccctta ttccatcttg
    36301 catgtcatta tttcccttct gtatatagat atgctaaatc cttacttccg catattctct
    36361 gagcgggtat gctacctgtt gtatcccaat ttcatacagt taaggacagg ccatgagtaa
    36421 gatttttgaa gataactcgc tgactatcgg tcacacgccg ctggttcgcc tgaatcgcat
    36481 cggtaacgga cgcattctgg cgaaggtgga atctcgtaac cccagcttca gcgttaagtg
    36541 ccgtatcggt gccaacatga tttgggatgc cgaaaagcgc ggcgtgctga aaccaggcgt
    36601 tgaactggtt gaaccgacca gcggtaatac cgggattgca ctggcctatg tagctgccgc
    36661 tcgcggttac aaactcaccc tgaccatgcc agaaaccatg agtattgaac gccgcaagct
    36721 gctgaaagcg ttaggtgcaa acctggtgct gacggaaggt gctaaaggca tgaaaggcgc
    36781 aatccaaaaa gcagaagaaa ttgtcgccag caatccagag aaatacctgc tgctgcaaca
    36841 attcagcaat ccggcaaacc ctgaaattca cgaaaaaacc accggtccgg agatctggga
    36901 agataccgac ggtcaggttg atgtatttat tgctggcgtt gggactggcg gtacgctgac
    36961 tggcgtcagc cgctacatta aaggcaccaa aggcaagacc gatcttatct ctgtcgccgt
    37021 tgagccaacc gattctccag ttatcgccca ggcactggct ggtgaagaga ttaaacctgg
    37081 cccgcataaa attcagggta ttggcgctgg ttttatccca gctaacctcg atctcaagct
    37141 ggtcgataaa gtcattggca tcaccaatga agaagcgatt tctaccgcgc gtcgtctgat
    37201 ggaagaagaa ggtattcttg caggtatctc ttctggagca gctgttgctg cggcgttgaa
    37261 actacaagaa gatgaaagct ttaccaacaa gaatattgtg gttattctac cgtcatcggg
    37321 tgagcgttat ttaagcaccg cattgtttgc cgatctcttc actgagaaag aactgcaaca
    37381 gtaatgccag cttgttaaaa atgcgtaaaa aagcaccttt ttaggtgctt ttttgtggcc
    37441 tgcttcaaac tttcgcccct cctggcattg attcagcctg tcggaactgg tatttaacca
    37501 gactaattat tttgatgcgc gaaattaatc gttacaggaa aagccaaagc tgaatcgatt
    37561 ttatgatttg gttcaattct tcctttagcg gcataatgtt taatgacgta cgaaacgtca
    37621 gcggtcaaca cccgccagca atggactgta ttgcgctctt cgtgcgtcgc gtctgttaaa
    37681 aactggcgct aacaatacag gctaaagtcg aaccgccagg ctagacttta gttccacaac
    37741 actaaaccta taagttgggg aaatacaatg ttccagcaag aagttaccat taccgctccg
    37801 aacggtctgc acacccgccc tgctgcccag tttgtaaaag aagctaaggg cttcacttct
    37861 gaaattactg tgacttccaa cggcaaaagc gccagcgcga aaagcctgtt taaactgcag
    37921 actctgggcc tgactcaagg taccgttgtg actatctccg cagaaggcga agacgagcag
    37981 aaagcggttg aacatctggt taaactgatg gcggaactcg agtaatttcc cgggttcttt
    38041 taaaaatcag tcacaagtaa ggtagggtta tgatttcagg cattttagca tccccgggta
    38101 tcgctttcgg taaagctctg cttctgaaag aagacgaaat tgtcattgac cggaaaaaaa
    38161 tttctgccga ccaggttgat caggaagttg aacgttttct gagcggtcgt gccaaggcat
    38221 cagcccagct ggaaacgatc aaaacgaaag ctggtgaaac gttcggtgaa gaaaaagaag
    38281 ccatctttga agggcatatt atgctgctcg aagatgagga gctggagcag gaaatcatag
    38341 ccctgattaa agataagcac atgacagctg acgcagctgc tcatgaagtt atcgaaggtc
    38401 aggcttctgc cctggaagag ctggatgatg aatacctgaa agaacgtgcg gctgacgtac
    38461 gtgatatcgg taagcgcctg ctgcgcaaca tcctgggcct gaagattatc gacctgagcg
    38521 ccattcagga tgaagtcatt ctggttgccg ctgacctgac gccgtccgaa accgcacagc
    38581 tgaacctgaa gaaggtgctg ggtttcatca ccgacgcggg tggccgtact tcccacacct
    38641 ctatcatggc gcgttctctg gaactgcctg ctatcgtggg taccggtagc gtcacctctc
    38701 aggtgaaaaa tgacgactat ctgattctgg atgccgtaaa taatcaggtt tacgtcaatc
    38761 caaccaacga agttattgat aaaatgcgcg ctgttcagga gcaagtggct tctgaaaaag
    38821 cagagcttgc taaactgaaa gatctgccag ctattacgct ggacggtcac caggtagaag
    38881 tgtgcgctaa cattggtacg gttcgtgacg ttgaaggtgc agagcgtaac ggcgctgaag
    38941 gcgttggtct gtatcgtact gagttcctgt tcatggaccg cgacgcgctg cccactgaag
    39001 aagaacagtt tgctgcttac aaagcagtgg ctgaagcgtg tggctcgcag gcggttatcg
    39061 ttcgtaccat ggacatcggc ggcgacaaag agctgccata catgaacttc ccgaaagaag
    39121 agaacccgtt cctcggctgg cgcgctatcc gtatcgcgat ggatcgtaaa gagatcctgc
    39181 gcgatcagct ccgcgctatc ctgcgtgcct cggctttcgg taaattgcgc attatgttcc
    39241 cgatgatcat ctctgttgaa gaagtgcgtg cactgcgcaa agagatcgaa atctacaaac
    39301 aggaactgcg cgacgaaggt aaagcgtttg acgagtcaat tgaaatcggc gtaatggtgg
    39361 aaacaccggc tgccgcaaca attgcacgtc atttagccaa agaagttgat ttctttagta
    39421 tcggcaccaa tgatttaacg cagtacactc tggcagttga ccgtggtaat gatatgattt
    39481 cacaccttta ccaaccaatg tcaccgtccg tgctgaactt gatcaagcaa gttattgatg
    39541 cttctcatgc tgaaggcaaa tggactggca tgtgtggtga gcttgctggc gatgaacgtg
    39601 ctacacttct gttgctgggg atgggtctgg acgaattctc tatgagcgcc atttctatcc
    39661 cgcgcattaa gaagattatc cgtaacacga acttcgaaga tgcgaaggtg ttagcagagc
    39721 aggctcttgc tcaaccgaca acggacgagt taatgacgct ggttaacaag ttcattgaag
    39781 aaaaaacaat ctgctaatcc acgagatgcg gcccaattta ctgcttagga gaagatcatg
    39841 ggtttgttcg ataaactgaa atctctggtt tccgacgaca agaaggatac cggaactatt
    39901 gagatcattg ctccgctctc tggcgagatc gtcaatatcg aagacgtgcc ggatgtcgtt
    39961 tttgcggaaa aaatcgttgg tgatggtatt gctatcaaac caacgggtaa caaaatggtc
    40021 gcgcctgtag acggtaccat tggtaaaatc tttgaaacca atcacgcgtt ctctatcgaa
    40081 tctgatagcg gcgttgaact gttcgtccac ttcggtatcg acaccgttga actgaaaggc
    40141 gaaggcttca agcgtattgc tgaagaaggt cagcgcgtga aagttggcga tactgtcatt
    40201 gaatttgatc tgccgctact ggaagagaaa gccaagtcta ccctgactcc ggttgttatc
    40261 tccaacatgg acgaaatcaa agaactgatc aaactgtccg gtagcgtaac cgtgggtgaa
    40321 accccggtta tccgcatcaa gaagtaattc ttgccgcagt gaaaaatggc gcccatcggc
    40381 gccatttttt atgcttccgc cagcggcggc aaaatcaatt catcgctctc atgctgctgg
    40441 gtgtagcgca tcacttccag tacgcgcaac cccgcacggt gcaccgcatc gtttaacgcc
    40501 ttccctttca gcaaaccact gataagctga gcacaaaaca ggtcgccagt ccctttcagg
    40561 tcggttttta cccgtgaatg ggaaatgaca ttcacgctgt cggcactgac caccaccacc
    40621 tgcatctcct gattttcttc attaccggag gcgctggtaa tcaccaccca ttttaatgtg
    40681 tctgaaagca gactttttgc ggcagcaatg gcactgtcga gatcgcggca atttttaccg
    40741 gtcaggattt ccagctcaaa gatattgggg gtaattccct gcgccagcgg cagtaaatat
    40801 tgtcgatacg cctcgggaag gtcaggtttg acataaattc cgctatcaat atcgccaatc
    40861 accggatcga ccatgatcaa taggtcagga tggtctttgc gtagcgcagt cagccactcg
    40921 gcaaggattt tgatttgcga tgccgttccc atatagcccg tggttacagc gcgcagctgg
    40981 cgcagcgcat cacgctcctg aagcgcacgc aaataaccgc taaaccattc atccggaatc
    41041 gcaccaccgt agaaagtgtc ataatgcggc gtattgctca gcaataccgt cggcacggca
    41101 aaaacattca gaccgttctg tttgatagca ggcacggcaa tgctgttgcc cacgctgccg
    41161 taaaccacct gcgactgcac ggcgacgata tccgcctgca gcgccctgct cttatcgtta
    41221 aacaacaaca aactactcat ttaatttttt ctccttgccg atgatcctca tcgtattcca
    41281 accgaaactt tacctgattc tggcagtcaa atcggctatc acaaaacaag gataaggtaa
    41341 ttcaatgaag aaaatcattt gtctggtcat tacactacta atgacactcc ccgcttacgc
    41401 gaagttaact gcccatgaag aagcccgcat caacgccatg ctggagggat tagcacagaa
    41461 aaaggatttg atatttgtgc gcaacggtga tgaacatacc tgcgatgaag cggtttctca
    41521 tctgcgtctg aagctgggca atacccgtaa ccgcattgac actgccgagc agtttattga
    41581 taaggttgct tcgtcgtcat cgattaccgg gaagccgtat attgtgaaga tccccggtaa
    41641 gagcgatgag aacgcacagc cttttttaca tgcgctaatt gcgcagacgg ataaaacggt
    41701 gcctgcgcaa taatcttaaa tccccgcccc ctggctaaaa tgctcttccc caaacacccc
    41761 ggtggaaagg tagcgatcgc cacgatcgca gatgatcgct accaccaccg caccagggtt
    41821 agcttttgcc acccgcagtg ctccggcaac cgcgccgccg gagctgacgc cacagaatat
    41881 tccttcccgc actgccagtt cgcgcatggt gttttctgca tcgcgctgat gaatatccag
    41941 cacctcatcc accagagaag cgttgaaaat ccccggcaga tattccgcag gccagcggcg
    42001 aatgccggga atactgctgc cttcttccgg ctgcaggcca acaatcgtta ctggtttgga
    42061 ttgttcgcgc ataaagcgtg aaacgccggt gatagtgccg gttgttccca tgctggagac
    42121 aaaatgggta atgcgcccgc cggtttgctg ccagatttcc ggcccggtag tggtgtaatg
    42181 ggcgtaaggg ttatcgggat tattaaactg atcgagcagc tttccttcgc cacgattcgc
    42241 catctccagc gccagatcgc gcgcaccttc catgccctgc tctttggtga caagaatcag
    42301 ttccgcacca taagcacgca tcgccgcacg gcgttcctgg ctcatgttgt cgggcatcag
    42361 caatttcatg cgatagcctt tcagcgcggc aatcattgcc agcgcaatgc cggtgttacc
    42421 actggtggct tcgattaaga catcacccgg tttaatttcc ccgcgctttt ccgcctcgac
    42481 gatcatcgaa agtgccgcac gatctttcac cgaacctgcc gggttattgc cttccagttt
    42541 taaccacact tcactgccgt tatccggccc cattcgctgc aacttcacca gaggcgtatt
    42601 gcctattgtt tgttctaatg tactcacgat ctctatccat acgtggtgtt gcctgatgcg
    42661 acgcttgcgc gtcttatcag gtctacaggt tacaaacctt gccattaaaa agcccggatc
    42721 gcgggaagcg cctccgggcg tttaacattc actcaaccta tcaggcgctt tgtgcgagag
    42781 caagttcctc atctcgggtt tcgatacgct cgtcgccgtt atacagccgc gcatgttgca
    42841 gaccaacgaa taaacgctcg ccacgctgcg gggcatcgtc gccatgcatc acgaccgtca
    42901 gcggttcgtt gtaccacccc agcggctgca ccactaattg ggtgtagtga cctttcgggc
    42961 tggcttccag tacctgtacc ggcagcggcg aatcgagact ggtacggcgg ctgatatcca
    43021 cttcccacgg gcgcaggaag agatccaccg gaccctgata cgcaggtgtg tagcccagcg
    43081 gccagcgatg cgcgccaaca tggaactgcc cgccgcgaat ggttccctgc aggcggttca
    43141 cttcgcccat aaattcgagt acaaaacggg tcgctggttc gcgccatacc tgattcggcg
    43201 catcagcctg ttcaatattc ccctgactca tcaccactac acgatcggct acttcggtcg
    43261 cctcttcctg gtcgtgggtc acgaacacgc tggtgaattt cagctcttca tgcagttgac
    43321 gcagccagcg acgcagctct ttacgcacct gcgcatccag cgcgccaaac ggttcatcaa
    43381 gcagcagaat ttgcggttct acagccagcg cgcgcgccag cgccacgcgc tgtttctggc
    43441 cgccggaaag ctgtgccgga taacgatctg ccagatgagc aagctgcacc atctccagca
    43501 attttgtcac tttcgctttg atggctgcgg cattcgggcg ctcgcgacgt ggcagcaccg
    43561 tcaggccaaa agcgatattg tcgaacaccg tcatatgacg gaacagcgcg taatgctgga
    43621 acacgaaacc gaccttgcga tcgcgcgcgt gcaggcggct gacgtcggtg ccgtggaagc
    43681 gaatatgccc gctggtttga tgctccagcc cggcgataat gcgcagcagc gtggttttcc
    43741 cggaaccgga cggccccagc aacgcgacca tctgacctga aggaatatcc agtgagatat
    43801 cgttcagcac ctgggtgcga ccaaacgact tcttaatatt ggcaatctca atgctcatga
    43861 tgttcctcct gctgtgcgcg tttatcctga ttctccaggc gccactgcaa catacttttt
    43921 aaaaacaggg tgataatcgc catcagcgtt aacagtgccg cagcggtaaa ggagccgacg
    43981 gtgttgtagt cctgctccag caattcaatc tgtaacggca gcgacagggt ttcgccgcga
    44041 atcgagccgg aaaccaccga caccgcgcca aactcgccaa ttgcgcgggc gttggtcaac
    44101 accacgccat aaagcagcgc ccagcgaatg ttcggcaatg tgacgcggcg aaacatctgc
    44161 cagccagacg cgccaagcaa aatcgccgct tcgtcttcct ggctgccctg gcttaacatc
    44221 accggcacca gctcgcgcac cacaaacgga cacgtcacaa agatggtgac cagcaccatt
    44281 ccgggccagg agaacataat ttgcagatta tgttcatcca gccagccgcc cagcgggccg
    44341 ttagagccgt agaacagcag atacaccaga ccggcaacca ccggcgatac ggcaaacgga
    44401 atgtccagta gcgtcagcag taactggcgt ccagggaagt taaagcgcgt caccagccag
    44461 gccagcagaa tgccgaacac caggtttacc ggtacggcaa tcagcgcgat catcaccgtc
    44521 agccagatgg cgtgcagcat gtccggatcg gccagattct gtaaaaccgg catcagcccc
    44581 ttgctgaatg cctgcacgaa gatgtaaatc atcggcacca gcaggatgaa cgctgaaacc
    44641 agcatcccga tgccaattag aaaccatttg ccccagttaa tcgggcgcgc gtcataacgc
    44701 ttcaattggg taacttccgc cattaatgac ctaccacacg ccgaccaaag cgactttgca
    44761 gagtgttaat tgagaacagc agcagcaggg atgccgcgag gatcaccgaa gcaatcgcgc
    44821 tcgccgccgg gtaatcaaac tcctgcaagc gcacaaaaat catcagcgac gtcacttccg
    44881 tcttccaggc gatgttcccg gcgataaata tcaccgcgcc aaactcacca agactacggg
    44941 taaacgacaa cgccacgccc gccaccagcg ctggagaaag ttccggcagc accactttgc
    45001 agaaactctg ccagcgcgtt gcaccaagcg tttccgccgc ttcttcatat tccgggccta
    45061 actcttccag caccggctgc acggtacgca ccacaaacgg aatgctggta aaggccatcg
    45121 ccaccgcaat accaagccag gtataggtga ctttgatatc aaacttcgcc agccattcac
    45181 cgtaaaaacc gtttacggaa aagagcgagg ccagcgttaa accggcgact gccgttggca
    45241 gcgcaaaggg taaatccatc agcgcatcaa gcagcgtgcg gcccggaaag cgataacgtg
    45301 ttaaaatcca cgccatcagc agaccgaaaa cgccgttaaa aatcgatgcc acaaacgccg
    45361 acagcagcgt cactttgtag gccgcgacca cttgtgggtt ggtgatcacc tcccagtact
    45421 gcgcccagct catctgagcc agctgcatca ccagcgcgga gagcggcagc agcaaaatca
    45481 ggcacacaaa cagcagactg gtgccgaggc ttaaggtaaa gcccggcagc acgcgtcttg
    45541 atgaaactgc aaacatcagt tacgccccgc cgctaacagc ttgtctaact cgccgccgct
    45601 ggtgaagtgg gttttcatca cttccggcca ggagccaaat ttgtcttcca cgcggaacag
    45661 ctcggtctgc gggaatttat ctttcagttt gtccatgact tccgggttat ttacgcggta
    45721 gtaatagtcg gtgatgatgg tctgcgcctg cgggctgtag agccagttca ggtaggcttt
    45781 tgctgctttt tccgtaccgt tggcctgcac gtttttatcg acccacgcca ccgggaattc
    45841 cgccagaata ttggttttcg ggatcaccac ttcaaagccc tgtgcttcat actgtttacg
    45901 gatgttgttc acttccgatt caaagctaat cagcacatcg cccaggccac gctcggcaaa
    45961 agtggtggtt gcgccacgac cgccggtatc gaacacttca acatttttca ggaactgggt
    46021 cataaactgt tcggttttgg ctttatcgcc accgtcagct ttatccgctg cgccccatgc
    46081 cgccagatag gtataacgcg cgttacccga cgttttcggg ttcgggaaaa tcagcttcac
    46141 gtcggagcgc accaggtcgt tccagtcgtg gatattcttc gggttgccct tacgcaccag
    46201 gaagcccatg gtggagtaga acggcgagct attattcggc aggcgcgact gccagtccgc
    46261 cgggatcagc ttgcctttgt catgcaggat ttgtacgtca gtcacctggt tataagtgac
    46321 aacgtcggct tttaagccct gtaaaatcgc cagcgcctgt tttgatgacc cggcatgaga
    46381 ttgtttaatc gtcagtttgt cgccgccgtt atctttggcc cattgctgtt caaacggcgg
    46441 gttcagggcg gcaaacagct cgcgggagac gtcataagaa ctgttcagca gttccgttgc
    46501 ctgtacatgg cctgccagca gcagagaagc gaccagcgcg agtgagttct ttttcagtaa
    46561 gttaacggcc attgcgcacc cttatatatt taatgacttt ctaatagcca tcatatttat
    46621 aacggacgtg aaaggagtaa cggttttata taccgtttgg tgatttggaa gttgaaaagg
    46681 gaataagaac cgggtttaaa acgaaatcct ccccacacat gcagggagga aacagaggtg
    46741 aatcagatac cgacgctaac cgtctccggc agtgtgctgc cgccatcaat cacattctgt
    46801 gtaccggtta aatagctgga ttcatccgat gcgaggaagg ccgccagttc gccgacttcc
    46861 agcggatcgg cgaggcgacg cagcgggatt gcttttgcca tatcagtcag caccgactct
    46921 ggatcttccg ggttcgactg gcgggcaatg ctttccgcca ttggcgtgcg gacgtacccc
    46981 gggcagatgg cgttaacgcg aataccggat tgcgcgtact ccaccgccag cgattttgtc
    47041 aggccaacaa tcgccgcttt cgttaaggcg tacgccgttt cacccggatc ggccaccata
    47101 tcaccagtga ctgaagacat catcacaatg cgaccatctt tgcgcgcaat catctctggc
    47161 agcaccgcct tcgtgacgtt ccatacgcct ttaatattga tgtcaatatg gaaatcgcga
    47221 tcttcatcgc tcatatcgag gaaactgccc agacgacaaa cgcctgcgtt attcaccagg
    47281 atatcaatgc gcccttcttt ttccttcgcg cgtttgatag ctgcggctac cgacgccggg
    47341 tcacgcacat cggcgacaac cgccgtacag cgatgaccac gaccacacag ttcgtccgcc
    47401 agcttttcga tctcagggga gatatccagc aagattaggt ttgcgccatg acgcgcaaaa
    47461 gttctggcaa ttccttcgcc aattccctgc aatgcgcccg taatcagtgc tgtcttgccc
    47521 gtgagtttac ccattttaat gctctccttg ttaatacaga gcgttacact ccattccctc
    47581 tgaaaataca ggataaacaa aatattgctg cggatcagct cactaattca ggaaaatctc
    47641 aatccagtcg ctgctttatg catcatttca ggcgttgtgt tagctcactg ctgcgctgaa
    47701 tcattttcaa tgactcgaca tcattgagtt gcacaagacc aacaaacagt aaatcggtaa
    47761 cagaattttg cgccgtgcgg gtagacatcg acgagctacg ccattcggtt tcaccggaaa
    47821 cggtatcgag cgtgaaatgc gccagccgcc gcagcggaga gtcagccaga gaagtaatgg
    47881 caatcaccgt tgccccctgc tttcgtgccg cttctgcgca caagacaatc tcttttttac
    47941 tgccgctgta agagatagcg atttgtacat cgcctttttt caacgcctgg gaaacggtgg
    48001 cttgtacgtg cgtgtcggct tcgcaggcca cgcgatatcc aattttcatc agtttgaacg
    48061 acagatcgcg ccccaccagc gccgatccgc ccaggcccgt tatctgaata aattgcgctt
    48121 tactgatgac atcaatgatt ttttgcagcc gcgcgtagtc gaacaacgcg caggtttgct
    48181 ccagcgccag ctctttttcg cgattcagtt tgcgggcaat cacttccagc gaatcatcac
    48241 tggtaatcga actgtgcagg tgcagcgccg tggcattcgt tttctcccgg ctggcactgt
    48301 attcgccaat taacgccata cgcaattcag taaatccttg tgcgccgagc ttctgggcaa
    48361 acttcacaat actcgactgg ctgatgccga gctgtttcgc catcttgcgc gaagagacgg
    48421 attgcagctc actcacgttg gcctgcagaa agtcagcgat tttttgctcg ttttcggtga
    48481 attcactccc cgcattgctt attttcgtca ggtacaacat cttccactct cctgggctgg
    48541 cagcataaat tccgcttaag ttcttatgac gctcttcaca ctctgcgagt gtagactcaa
    48601 tgattccttt aagtctgtca attcgggata tatgattcca tacatctgca attatttgat
    48661 cgtaaatagt aaggtcacca ccgatgcaac tagaaaagat gattactgaa ggctcgaaca
    48721 ccgcctcggc tgaaattgac cgcgtatcga cgctggaaat gtgccggatt atcaacgatg
    48781 aagataaaac cgtaccgctt gccgttgagc gcgtcctgcc ggatatcgcc gcggcgatcg
    48841 atgttatcca cgcccaggtc agcggcggcg gtcgtctgat ttatctcggt gcgggaacat
    48901 ccggtcgtct ggggattctg gatgccagcg aatgtccgcc cacctacggc gtgaaaccgg
    48961 gtctggtggt tggtttgatt gctggaggcg aatatgccat tcagcacgcg gtggaaggcg
    49021 ctgaagatag ccgggaaggt ggtgttaacg atctgaaaaa tattaattta acggcacagg
    49081 atgtggtggt tggcattgct gccagcggtc gcacgccgta tgtgattgcc ggactggaat
    49141 acgcacgcca gctcggctgc cgcacagtgg gaatttcctg taatccgggg agtgccgttt
    49201 ccaccaccgc cgagtttgcc attacaccga ttgtaggtgc cgaagtggtc actggttctt
    49261 cacgaatgaa agcaggcaca gcgcagaaac tggtgctcaa tatgctttcc accgggctga
    49321 tgattaaatc tggcaaagtg ttcggcaacc tgatggtcga tgtggtcgcc accaacgaaa
    49381 aactgcatgt gcgccaggtc aatatcgtta aaaacgccac cggatgtaat gccgaacaag
    49441 cggaagcggc gttaattgcc tgcgagcgca actgtaaaac ggccattgtg atggtgctga
    49501 aaaatctcga tgccgcagaa gctaaaaaac gcctggatca acacggcggc tttattcgtc
    49561 aggttttaga caaggaataa cccatggcca aagagatcag cagtgaactt ctgaacacca
    49621 ttcttacccg tgtcggcgga ccgggaaata tcgccagttg tggtaactgt atgacgcgcc
    49681 tgcgtctggg tgtacatgac agttcactgg ttgatcccaa tatcaaaacg ctggaaggcg
    49741 tgaagggcgt cattttgacc agcgaccagg tgcaggtcgt ttttgggcct ggtaaagcgc
    49801 atcgtgccgc aaaagcgatg agcgagctgc tgggcgaggc accggtacag gatgccgcag
    49861 aaatcgccgc ccagaacaaa cgtcagttaa aagccaaaca aacctccggc gtgcaacaat
    49921 ttctcgccaa attcgccacc atcttcacgc cgctgatccc cggttttatt gccgccggtc
    49981 tgctgctggg gatagcgacg ttaattgcca cggtgatgca cgttccggca gatgctcagg
    50041 gcacgctacc cgacgcgctg aattttatga aggtgttcag caaaggtttg ttcactttcc
    50101 tggtgattct ggtgggctat aacgccgccc aggcatttgg cggcacgggc gtcaatggcg
    50161 caattattgc cgcactcttt ttgctcggtt ataaccccgc tgcaaccacc ggttactacg
    50221 ccggttttca cgatttcttt ggtctgccca tcgatccgcg cggcaatatt atcggcgtgc
    50281 tgattgccgc gtgggcctgc gcccgcattg aaggcatggt gcgccgcttt atgccggacg
    50341 atctcgacat gctgctgacc tcgttaatca ccctgctgat caccgctacg cttgcgtacc
    50401 tgatcattat gccgctgggt ggctggttat tcgaaggtat gtcatggctg tttatgcacc
    50461 tgaacagtaa tccgttcggt tgtgcggttt tagccgggct gttcctgatc gccgtggtat
    50521 ttggcgtgca tcagggcttt attcctgttt acctcgcgtt aatggacagc cagggattca
    50581 acagcttatt tcccatcctt tcaatggcag gcgcgggcca ggtgggcgcg gcgctggcac
    50641 tctactggcg ggcgcaaccg cacagtgcat tacgcagtca ggtacgcggg gcgattattc
    50701 ccggtctgct gggcgttggt gaaccgttga tttacggcgt caccctgccc cgcatgaaac
    50761 cgtttattac cgcctgttta ggcggcgcag cgggcggttt gtttatcggc ctgatagcct
    50821 ggtggggtct gccgatgggc ttaaacagcg cctttgggcc gtctggtctg gtggcgctgc
    50881 cgctgatgac ttccgcacaa ggcatccttc cggcaatggc ggtttatgct ggcgggattc
    50941 tggtggcatg ggtttgcggg tttattttca ccacgctctt tggctgccgt aacgtcaatc
    51001 tggactgata tcatgaaacg gacaatgctc tatctttctc tgctggctgt tagttgtagc
    51061 gtcagcgccg caaaataccc tgttctgaca gaaagttcgc cagagaaagc agggttcaac
    51121 gtcgaacggc ttaaccagat ggatcgctgg attagccagc aagtggacgc cggttatccc
    51181 ggcgtaaacc tgctgatcat taaagataat cagattgtgt atcgcaaagc ctggggagcg
    51241 gcgaaaaagt acgatggcag cgtgttgatg gaacagcctg tcaaagccac caccgggacg
    51301 ctgtatgacc tggcctcaaa caccaaaatg tacgccacga acttcgccct gcaaaagctg
    51361 atgagcgaag gcaaactgca tcctgacgat cggattgcga aatatattcc ggggtttgcc
    51421 gatagtccaa atgacaccat caaaggcaaa aacacgctgc ggatttctga cctgctgcat
    51481 cacagcggcg gtttcccggc ggacccacaa tatccgaata aagcggtcgc gggcgcgtta
    51541 tattcccagg ataaaggcca gacgctggag atgatcaagc gcacgccgct ggagtatcag
    51601 cccggcagca agcatattta cagtgatgtc gattatatgc tgcttggatt tatcgtcgag
    51661 tcggtcaccg gtcagccgct tgaccgctat gttgaagagt cgatttatcg cccgctcggc
    51721 ctgacacata cggtatttaa cccgctgctg aaaggcttta aaccgcagca aattgccgcc
    51781 acggaattga acggcaatac ccgcgatggc gtgatccatt tcccgaatat ccgcacctcc
    51841 actctctggg gtcaggtgca cgatgaaaaa gccttttatt cgatgggcgg cgtttccggg
    51901 cacgcaggtt tgttttccaa taccggcgat attgcggtgt taatgcaaac gatgctgaac
    51961 ggcgggggct atggcgatgt gcagctgttc agtgcggaaa cggtgaagat gttcaccacc
    52021 agctctaagg aagatgccac ttttggcctc ggctggcgcg tgaatggtaa tgccaccatg
    52081 acgccgacgt ttggcacgct ggcaagcccg caaacctacg gtcacactgg ctggaccgga
    52141 acggtgaccg ttatcgatcc ggtgaatcat atggcgattg tgatgttaag caacaagcca
    52201 cattcgccgg ttgccgatcc gcaaaagaat cccaatatgt tcgaaagcgg tcagctgccg
    52261 attgcaacgt atggttgggt agttgatcag gtgtatgcgg cgttaaagca aaagtaataa
    52321 aaaggccagt cagtaatgtt actgcctggc ctttaatcaa tgaatcagaa acgcttacag
    52381 cgccatcaac ttgtccagcg acggtgcgaa ataatagccg ccggttaccg gtttggtgaa
    52441 acgcaacatc gcatcacgct taccatcggt atcgccaaac atgctcagca gttgctgctc
    52501 aatgttatgc agacgcgcgc agtaggcgca gaagtacaga ccgtgagtgc cactggcagt
    52561 gccgtacggc aggctctggc gaacaatctt cagccctttg ccatcttctt tcagatcaac
    52621 gcgggtgagg tgagaggttt ccggacgttc gtcgccgtcg atctcttcgt tggcctcttt
    52681 ggtgcgcccg atcatcatct cctgatcgtg aacgctcatc cggttgagct gcttcaggtt
    52741 gtgttcccaa cgctggacaa acacatagct gccgcccgca tccacgccgt ctttgataac
    52801 cgccacttcg cgacgcgtct cttcacccgc cgggttttcc gtaccgtcaa caaagccgct
    52861 caggtcacgc tcttcaaccc aacggaagcc gtggatctct tctttcactt caatgcagtc
    52921 accaaaggct tccatcgccg cctgggcgac agagaagttt acgtcgtgac gcagagaaag
    52981 aatgtggatc aacacatcga actgggtggt cggcgcaagg cctttaccgt agcccggaaa
    53041 atctttcagc tcttctgccc caacgccgcc gctcagagcg cgccaggtgt tgttaccaaa
    53101 ggcaaccacc gcaccaagat gcgcgtccgg gaatttcgct tcaaaagttg ccagtttgtc
    53161 ggcaaatgtt ttactggccg cacgcagggc gtcaacttcc cctttcacgt tggcttcgat
    53221 ccaaatcgcc gcgcggcaat gttctggcaa aatgccactc tgaacctgag acattgttcc
    53281 tcctgaaaat aataatgcca cgcaatccgt ggcattgttg agcgctattt tacgtgtttt
    53341 ttcgcggcgg cggtttgttc agacgcaaat taacgccgcc agataatttt actgactttc
    53401 cagtttttca gggtatcgtc cgaaggcatt aacccttcag gaccactcca ttccccgctg
    53461 aactggtagc taatatgttg actgccttcg gctttacatt cgacggcacg attatcatca
    53521 ccatcagctt tttggcaatt gccaaatgct ttgctgtaaa ggtcgctaaa cggtgtaccg
    53581 attttaacac cggtgtcagc aggaatatcg ctatccagca cgtcaatgcg actgatcgtg
    53641 ccttgatcgc cgttaatcac catcgccacg ttgtcgccct tcatcacttc aaaaaagcgc
    53701 accacgttgc cgttcgcggt tttcattccg ctgcgcaggc gataatcgcc atcaagcgca
    53761 tcggcaatgg cttgttcttg cagtggtgtg gacgccgtta attcaccaac gccctgctcg
    53821 ctcactttgg tggacgaccc aaaccagttc cacgggttag cggccgacca gttaactgac
    53881 gacatcgtgg aacagccggt cagcatcaac ggcatagcgc ataacattaa acgcagcgat
    53941 ttcatgtcac ttcctttggt tattcaataa cgttgcttgg agtgcaaatt caccaaaaag
    54001 tgccgttatt ctttttcttc attaaaacaa gcgcgtaaac gtcgattggt cagcagccag
    54061 attaacgcca caatatccgc cactaccagc gccagaccga taccgctaac ggattcaccg
    54121 ttcagccaca gccacggttg ccagcaaagt aaaaccacct gcgccaacag caacagaaaa
    54181 tagagcacat gccaagtgcg aggaaatgta gcccgccgac cgctcaacaa aaacgccagc
    54241 accgccggaa tgcctggaat cagccccagc cagaaattat cgtgatcggg ataaaacaga
    54301 tttagcagcg cagtaccctg ctcgcgcgac gcaccggcaa tgacaaacag cacccaggtt
    54361 cgcgcctgaa gcaatagcac aagccagaag agcaagggta aacgcaggcg tccgtgcgca
    54421 tcataatgga caggatggaa ctcagtactc ttcatcttca atcaaacgct tacccagact
    54481 cagcacgtcg gcgtgttcat atcccaggcg ttcatacatt ccgagcacca tgtcgttatc
    54541 ttccggcaca ttgatctgaa ttttcgggca gccacgagca atcagctttt tctccagccg
    54601 attaagcaac gcattggcaa tcccacgccc acgaaactct ggatgcacgc caagataata
    54661 agcagacccg cgatgcccgt cataaccgcc catcaccgtt ccgaccactt caccgtttac
    54721 ctcagcgacc aaaaacaaac tgacgtcatg gttcatctta cgctcgatgt ccatttccgg
    54781 atcgttccac ggacgcagca agtcgcaacg ctcccaaagg gtgatgacct cttcgaaatc
    54841 ttcctggcga aatacgcgta tctccatggt attcgttacc tttttgcggg ttaaaaggct
    54901 gattatggcg tgaacggtcg aattagccaa tatctgacga aaatcggttg aaaaagtggc
    54961 ataatgggga gttgtcaact attgaaatga aaagtaaaac aattctcaac atcaaaccgt
    55021 cgtaacggat tacgcgatac gatataacat ctggaacttt attattacaa ctcaggccgt
    55081 atgagcactt ttaaaccact aaaaacactc acttcgcgcc gccaggtgct gaaagccggt
    55141 ttggctgccc tgacgttgtc aggaatgtcg caagccatcg ccaaagacga acctttaaaa
    55201 accagcaacg gacacagcaa gccgaaagcc aaaaaatctg gcggcaaacg tgtcgttgtt
    55261 ctcgatccag gccacggcgg aattgatacc ggagcgatcg gacgcaacgg ttcgaaagaa
    55321 aaacatgtgg tgctggcgat tgctaaaaac gtccgttcca ttttgcgtaa tcatgggatt
    55381 gatgcgcgtt taacgcgttc tggcgatacg tttatcccac tttacgatcg cgttgaaatc
    55441 gcccataaac atggcgcaga tctgtttatg tcaattcatg ccgatggctt taccaacccg
    55501 aaagctgccg gtgcttcggt atttgccctc tctaaccgtg gggcaagtag cgcaatggcg
    55561 aaatacctgt ctgaacgcga aaaccgcgcc gatgaagttg ccggtaaaaa ggcgactgac
    55621 aaggatcacc tattgcagca agtgctgttt gatctggtgc aaaccgatac tattaaaaac
    55681 agcctgacgc tcggctcgca tattctgaaa aagattaagc cggtgcataa actgcacagc
    55741 cgcaacaccg aacaagccgc gtttgtggtg ttgaaatcgc cgtcggttcc ttcggtgctg
    55801 gtggaaacct cgtttatcac caacccggaa gaagagcggc tgttaggcac ggcggcgttt
    55861 cgtcagaaaa tcgccacagc gattgctgaa ggcgtgatca gttatttcca ctggttcgac
    55921 aaccagaaag cacattcgaa aaagcgataa gttatgaaac ccgacgcaca ccaggttaaa
    55981 cagtttctgc tcaaccttca ggatacgatt tgtcagcagc tgaccgccgt cgatggcgca
    56041 gaatttgtcg aagatagttg gcagcgcgaa gctggcggcg gcgggcgtag tcgggtgttg
    56101 cgtaatggtg gtgttttcga acaggcaggc gtcaactttt ctcacgtcca cggcgaggcg
    56161 atgccagctt ccgccaccgc tcatcgcccg gaacttgccg ggcgcagttt cgaggcgatg
    56221 ggcgtttcac tggtagtgca tccgcataac ccgtatgttc ccaccagcca cgcgaatgtg
    56281 cgttttttta ttgccgaaaa accgggtgca gagcccgtct ggtggtttgg cggcggcttc
    56341 gatttaaccc ctttctatgg ttttgaagaa gacgccattc actggcaccg caccgcccgt
    56401 gacctgtgcc tgccatttgg tgaagacgtt tatccccgtt acaaaaagtg gtgcgacgat
    56461 tacttctacc tcaaacatcg caacgaacag cgcggtattg gcgggctgtt ctttgatgat
    56521 ctgaacacgc cagatttcga ccactgtttt gcctttatgc aggcggtagg caaaggctac
    56581 accgacgctt atttaccaat tgtagagcga cgtaaagcga tggcctacgg cgagcgcgag
    56641 tgcaattttc agctctaccg tcgcggtcgt tatgtcgagt tcaatctggt ctgggatcgc
    56701 ggcactctgt ttggcctgca aactggcggg cgcaccgagt cgatcctgat gtcaatgccg
    56761 ccactggtac gctgggagta tgattatcag ccaaaagatg gcagcccaga agcggcgtta
    56821 agtgagttta ttaaggtcag ggattgggtg taactccctc acccccattc ccgcatccgc
    56881 tgatgcaacg tcaacgacgg cttctcggca aacagctgct ggtaatccgt ggcaaattgc
    56941 cccagatgcc agaatcccca ctgcatggcg gcgtctttta ccgttgtgct ttgcgaccac
    57001 gggcttatca gttcgcggcg tacggcgttc aggcgaatgc gtttcagcca cgcgtttggg
    57061 ccaatgccta aaatagcgtg aaacgcgttt tgtagcgtgc ggcgactgac atgcagttga
    57121 ttacacaagt ccagcaccgt caccggctca gacatatttt ccagcacata ttcacgggcg
    57181 cgggaaagta atctacggta actctgatga ctgatgcttt ccgccgtcac cattggttgc
    57241 gcgtcttcca gcatcgcccc catcgccatt agcaaattat cccccagcac ttttcgcact
    57301 gcaggctgat ggagattttc cgggttctcg caaaacgtcg ccagcgcctg ttggacaaag
    57361 ccccacagcg cggctttatg ctgctctttc acttccagcg ccgactggct acgcagcata
    57421 tgtaataccc gatccgggtt atgcaaaaag ttagcctgcc gggtgatgac atcttcagaa
    57481 agcaccacgc ccaggatcgt gtaatcatcc ggcgtgctca gttcaaattc agtgccacca
    57541 gggcgggtgg cgatttccgc gcttcccaga cattgcgaac cgataaatcc ctgctcaccg
    57601 cgcgtcgccg gaatgccaaa ccagaacgag ttcggccaga ccaggcacga ctgacgcagc
    57661 gccagaccgg tgtattcacg aaaaacctga atatcatcga gtagaatttc cgtaaattca
    57721 caatgaaact tgcccggatg cagctgatcg taaatctgct gccaggcggt aatcgttaaa
    57781 gcatgttcat agacatccgt tgtctgtcgt tgatgaacat tatccacttc gaccttcggc
    57841 gtgagcttca ggttttcggg taagggttca tgataaagat ggtgcaaatt ggctgtacgg
    57901 gtctttttca tgatgttaat gccgggtgtt gtaggacacc cggcacctcc gacaggttaa
    57961 tggggcttga gacgataacg actactgcgt ttacgtaacg tcccggcaga aaagagctgt
    58021 tcgagcgcat ttctggcttt ttccagcggc cagccaaagt gggcagccac ttctcctgcc
    58081 gtcattccct gacgtactga tgttaacagc gccaacaact cgtcagcaga ttccgcgacg
    58141 ataactggcg cgtcgggttc ccttacgggt tgcttcggct ggcggttaaa gccagtaacc
    58201 agccactggg tgtcatcgtc tggccgaccg atctccttgc ggctgatgac acgcccggta
    58261 cgcatcgcgg cagcacaacc tgcgtccagc gccgcacgac acgccgccag atcgccttcc
    58321 accaccagcg ttaagcgacc agggtcaagc acttcgtgac tgagcagacg aacgttagct
    58381 gctttgagca tggcatccgc tgcatctatc gcggcgacca tgccgtccac ttccagcaat
    58441 cccagtgcat tgatcatcgg caacctccgt tacgcacgct ggattggatt acgcgcgatt
    58501 tccagcactg catcggtaaa ggcgttacag gctgctttac acgcggcctg gctaccggtt
    58561 aaaaatgctg ccgagtagtt ggtttcagac ggtggcggga cataggtcac cagctgtacg
    58621 tcggcagatt tcaacgctgc atcaatgccg taggtcgctt ccagcggcgg tgccaccaga
    58681 tacgccatcg gatcgccaag cgtgatcccg gcggttgatg agagataaga accggtacgc
    58741 gaaactacat gtgccaggaa ggcggtgttt tctgcgtcat tggcccactg gaaagctgcg
    58801 ccattttcaa tatgtgcaac catcgcatcc agaccggcac gcacttccgc cgggtttggc
    58861 ccaccaagca taatcagcac ttcaccggca gtcggtgacg ggccgtgtgc cgctcccgca
    58921 tacagcgagc gaccatatac cacttcgacc atcgcttgtt tggtcgcttc gtcagccgca
    58981 atgtaggtga cgtcgtcaga atcggcagaa atcagcccga ggctacgaat atgcggcggc
    59041 aattttagtt cacgcgcaaa ttcagcgtta acggaggcaa tcacccgcat ggcggttacc
    59101 gacggtcgaa tcaaatctaa agctggcatg atgcctcctt atcgggtcat gttgatgccg
    59161 gatgctttct gctccagcat acgtttcgcc aaatccacaa tgacggctgc ggcttcaacc
    59221 ggcggcgtgc cgccctggtg aatgttagag atacaggtgc gatcggcctc aacggtggtc
    59281 gccatacgcg gcgagtaaac ggcgtagcag gagaggcttt ctgactgccc taaccccgga
    59341 cgttccccca ccagcaggat caccactttc gcgccgagga tctcaccgat ctgatcttca
    59401 atcttcacgc gaccataacg aacaaagaat ggcgtgccga ctttcagccc ggcctgtttc
    59461 aggcccgcca tcagcggcgg caggatctct tcgtagttca cggtgatggc atcagttgac
    59521 aggccatcag aaataacgac ctgtacgtcc ggattagcaa cacactgtgc tttcagcgct
    59581 tcaacagctt ctgcacacag gcggcggccc atatccgggc gagtcaggta gagatttttg
    59641 tcgctaattt cggagcgtac ttccagcaag ccctgcgctt tcacccactc ttccggcact
    59701 tctttcagta cggtatcttt cgaacgagag tgatcggcca ggaaacgcag caacgcctgc
    59761 gtacgcggac gcggaccggc acgaccggta caaacgcggg ccacggtgct gcgacgcagt
    59821 tctgttaata cgtctgcgcg atgcgggttt tcaacaccaa tccacgcttt tgcttccgca
    59881 gaacctaaat ccagcgcgca gctttccgag gtcaccggtg ccgcacagtt ggtggtggcg
    59941 cactttgctt ctgacggcgc gggggccgtt tgtcccattg acgccatcac gctgcgtaca
    60001 atttcttcaa tctgtttttg atccatgatg tgttatctcc gcgtcatcag aagaacagtg
    60061 acggatcgcc cgcccgtttg gtcaggcgac cgtttgccat aatgcccatg ctttccagcc
    60121 agcgttcaaa ctccggtgac ggacgcaggt tgagtaactg acgcacagtg gcggtatcgt
    60181 ggaatgcggt ggtctggtag ttgagcatga tgtcatcacc cagcggcatc cccatgatgt
    60241 agttgcagcc tgcggtggcg agcaggatca tcaggttttc gttgaggttc tggtcagcgt
    60301 cagcgtggtt ggtgtaacag cagtcacagc ccatagagat gccgctcagc ttgcccataa
    60361 agtgatcttc taagccagca cggataatct ggcggtcgtt gtagagatac tccggcccaa
    60421 taaagccgac cacggtgttg acgataaacg gatcgtaatg acgcgccagc ccatagttac
    60481 gtgcttccat cgtcacctgg tcagcgccga agttagcgcc agcggatagc gcagagcctt
    60541 gtccggtttc gaagtagagg cagttttccc cggcgatacg gttgaactct gcacccactg
    60601 cgcgcgcttc gtcgagcatc gccagttcca cgccaaactc tttcagccct ttttcgctgc
    60661 cacagatact ctggaaaatc agtccgccag gcgcgccgcg acgaatcgct tcgatctggg
    60721 tggtgacgtg cgccagtacg cagccctgag ttgggatgtt gaatttgtcg atcacgccgt
    60781 aaatggtgtc caacacgcgg cttaagtttt ccacgtcgtc agttaccggg ttaacgccga
    60841 tcaccgcatc gcccacccca aaggaaagcc cttcatagat ttgcgcagcg atactttgca
    60901 cgtcgtcacg ggtatcgttc ggctgcaaac gggcgctgaa ggtgcccgga ataccaatgg
    60961 tggtattggc ctttttgatt accggcattt tcttcgcgcc gtagatcagg tccgcgttgg
    61021 agcaaatctt cgctaccgcc gcgaccactt ccgaggtcag ccctttgcgg gtaaaggcaa
    61081 tgtcgtccac gctggtttca tcgctcagca catactcacg cagttcgctg atgctccagt
    61141 ttttaatctg gttgtaggcc gtttcgttaa catcgtcctg aatcagccgc gtcacgcagt
    61201 catcttcata ggcaatcacc ggattattgc ggatgtccgc tacggtcatt tccgacaaca
    61261 cctgctttgc cgccacgcgc tcctgtgagc ttgccgctgc aacgcccgcc agcacatccc
    61321 ccgaacgcag ttcgttggct ttagccagca cctcttttac atccttaaac tgatatacat
    61381 tgccgaacaa tgtggtcttt agtttcataa gtcgttccct caggaaggaa atgcgagtga
    61441 tttcaccgtc accggcacaa ccgatccgcc aaaaagaggc gtaccaatgt cgatatagtc
    61501 ccccgcgcgg acaatcactt cgtcaatgac tgccaacggg agttgttgta gctgtgggcg
    61561 caacaacatg cccagagctt taccaaagtc ctgcccggcc accaccagca ggggatgcgg
    61621 attcggaaaa cgcgcgacga aatcgaccag cgcgttgatg accgtcagta ccgcggcgta
    61681 acgcacaggc agcgaggcgg gaagcgccag cacgtacgcg tcagttttgg gatcaagatc
    61741 cagctgaagc agcgcctgtt gccaggcact caccagatcc gtttcatcaa tcgggatcgc
    61801 caccggcaaa ttgcgcagcg gcagttgtac gccctccagc cagattgtgc tgccagagag
    61861 cgaaagggta tgtgcacccg cgccaatcac cgtggcgcgt acggtttgcg ccggaaactg
    61921 cacattcatc tcacgcaggc gcggatggtc atgcagcgcc gttgccagaa gtgggccaat
    61981 atcggcaaaa cagaacgggt cggcgggctg gtggcgataa cattcgccca cgcctccaga
    62041 aagcgtaatg atttcgggcg taacacctgc gggcagcaaa ccggtttgca tcaatgcctg
    62101 cgcgagcggc gaaagcgttc cgtcaatcac ttcgacaatc agcgccgcca tccgccgggt
    62161 aacctgcacc agctgcgcgc cggtcagcga acgggcgtca gtgcctgcac cgaagcactc
    62221 atccacaatc atctgccccg gtttatgagc gtaaaccacg cgcccctggc tgtcggtttc
    62281 catcaggcga ccaccgacgt tgagacaggc agtgccgctg atttttccgg catcgaacag
    62341 ggcgtagttc gcggtgccac cgccgatgtc gatattcagt acccgacaca gccgttgttc
    62401 agaaagggtt tgcgccccag ctccgtgacc ggcgatcacg gattcgaggt gcggcccggc
    62461 gctggcaacg acaaaatccc ccagcgattg agagagcgcc atcaccgccg ggcgagcatt
    62521 gcgggttttc gcgctttcac cggtgatgat gatggcacca gaatcaacgc tttccggcgc
    62581 aatacccgca gcctgatatt gctcgagtat taaggttttc agttccgctt cttttaaacc
    62641 gccctgttta tcgacagggg taaagaacac cggactttgc cagctaattt cgcgtttaat
    62701 gaattcgtag cgcggcacct gcgacaccgc cgcacggtta accagctcca ggtgggagaa
    62761 aatcacctgg gtggtggtgg tgccgatatc gataccgacg ctcaatagct ggcgagtgtt
    62821 cacgattgtg cctccgcttc ggttttagtc gcggtcgcgt cttcttttgg caccagcatc
    62881 atcgccacgc caatcgccgt tacgccgccg atcaacttgc cgacaatcat cgggaagatc
    62941 atggcgttca tgttggcagc ggcgaagcct aagtggtcgc ccagggcgaa agcagcggaa
    63001 acggcgaagg cgcagttgat gactttgccg cgggtatcca tctgcttcat catgccgaac
    63061 atcgggatgt tgttggcaag cgttgccacc atgccggctg ccgcgatgtt gttcatattc
    63121 agcactttac cgacgctcat cagcggtttt tcaaaccagc gagtcagcag cagcaccatt
    63181 ggatacgccc ctaacagcac gcaggagata gaaccgataa cttcaatggc gcgcatcact
    63241 tcaccgggtt tatcgccagg ggccataaag ataggatcca gtccggggat cagttcccag
    63301 ccaaggagga atttcactac cgcagcggca agaccgaggg tgatcaatgc cacgaggaat
    63361 ttggcgaaga tctggaagcc gttgatcatt ttttccggga tgaatttcag ccccagcgcc
    63421 accagaatcg cgacaatgat caccgggatc atgttcatca ggatcagggc gaaagtgaat
    63481 tccaccggct gaccgttgat ctgcacaccg gagtacatag caaccagacc accagcaata
    63541 cagccaatcg gaatggtcac aatgcccgcc agcacgccga gcgccagata acgacggtca
    63601 gaaggttcga taatgccgag cgccaccgga atggaaaaca caatcgttgg ccccatcatc
    63661 gacccgagaa ttaacccaga gtatagccac gctgctacgt cgccgcccgc cagctctttg
    63721 gcgaggaaga agccgcccat atcgcacgcc agcagtgttc cggcgaacat cgatgggtta
    63781 gcgccgagca tttcgtaaac cggaataatt accggcccga gtacgtgagc cagtaccggt
    63841 gccagcgcgg tcataccgac catcgccagg cccagtgcgc ccattgccat aaagccttct
    63901 tcgaactgac cgcctgatcc ttcgatactt ttaccgaact taccgaggaa acgagcagaa
    63961 ccgccgaact gcgacaggat cctgtctacg gcagctatca gcataaagaa catcatgatg
    64021 tacatgatga tttcgttaat tcccatcgcc tttactccct gttagttgtt atttattggc
    64081 ggatgcggcg taaacgcctt atccgcccta catgtgcaat cccgaaggcc ggatcagacg
    64141 cggcaagcgt cgcatccggc atttattgcg ccgctgcgta caggccgaca atctgctcca
    64201 ggctggcggt acgcgggttg ctgcgcagac aaatatcttc ctgcgcggcc tgcgcccatg
    64261 caccgtaatg cgcagatgtc gcgccaacat cgcccagtcg tttaccaatc ccaacttccg
    64321 caatcagctc acttaccgcg ttaatagcgt cacgatcgtc ggatttttta gttcgcagtg
    64381 cccgaccaat ctgactaaac cgttcgcgac aaaccatccg gttaaattcc atcaccgttg
    64441 gcagcaacat ggcgttcgcc agaccgtgtg gaatatgcag cgccgcccct ggctgatgcg
    64501 ccatcgcgtg gcacaaccca agccccgcac tggaaaacgc cattcccgcc atacatgaag
    64561 ccagtaacat gctctcgcgc gcggcaaggt catgaccgta gcccaccgct ttcggcagcg
    64621 atttgccaat catcgcaatg gcaccaatcg ccagactgtc ggtaaacggt gtagcgttca
    64681 gggcgctgta tgcttcaatg gcatgggtta acgcatcaat gccggtcatc gccgtgacat
    64741 gcgacggcac accttcggtc aatgcggcgt cgagaatcgc cacatccggc atcagcgagg
    64801 catgggctaa cacctgctta cgcccgctca ccgcatcgat aatcaccgtt acgttggtgg
    64861 tttcagagcc ggttccggcg gtagtgggaa tggcaatcaa cggcaggcgc gattgcagaa
    64921 cgctggtttc tgacatctct gccagcgtgc tatccgggtt cgtcaccagc aacgccacgg
    64981 ctttcgccgc atccagcacc gagccgccgc caaatgcgat caccccatca cagcctgact
    65041 cacgcaactg cgccacggct gcacacacgt cggtgatgca cggttcgccc accggacatg
    65101 gccagagcgt catggcgata cctttaacgg ccaggctacg cgtcagcccg gcggtcatcc
    65161 ctgcctgatg caaaaagctg tctgccatca cgaacagatg tttcagccca cgcgtttgcg
    65221 cttgctgtcc gcaactgctc accgagcccg gaccgcaaag cgtcaccggt ggaacgctaa
    65281 atgtttttac ccgttgcaga ttcagggtat cgaacgcctg aaagagcgcg gtctgcaatt
    65341 cattttgcat atagcccctc cgctttctct cgcccactac tggcgatcgc cagcggggtc
    65401 ataaacaaac tgtgctgcgg taaatgcacc tgtaacgccg ggaattgttt gcgaaacagt
    65461 tccgccacgc ccggttgcat acaagagccg cccgccagcc ataaatcagt aatgccctgc
    65521 ccttcaatat gccgggcgac aatgtccgcc attttttcgt acaccggttt caccgcgggc
    65581 caaatctcgt caccatgacc gcgcttgtac tgctctgcct cttccagcga aatacgacga
    65641 tttccggcga gagtcagaga gatgtgatgt ccgccggtgg cttcatccgc cgagtacgtc
    65701 accttgccct ttttcacgat ggcaatgccg gtagtgccgc cgccgatatc cactacaccg
    65761 gcgttgtcca gttgcagcag atccgcgact gcggttggct catccagcac atggctcact
    65821 tccagcccgg cagattccag cacgttgatg gaaatacgcg ggtccgtacc tggaggaaat
    65881 gaagtcgccg catggctaaa acgaagaccg aattgctgct caagagtgtc gagatggcga
    65941 cgaacaatgg tgaccgcacc gaagaaatcc cagacgatgc cgtcgcggac cacatcggcc
    66001 cagtcgaggc ataccgccac cggctgaccg tcgcggtcaa caaccatcga caccacatcg
    66061 caggtgccca gatccacccc cagccacagc ggagattccg tcgcggcagg cgtctggtta
    66121 cacagcgttg ccgccgtttg cagacgtggg gtgagccatt gttcgtcgtg cgccatcggt
    66181 tactccttaa acaatgcgaa acgcatcgac taatacacag cgacgcagac ggacaaacgt
    66241 ccgcgcgctg gttacccctt caccggttgg cgtggtgatg gtcatggtgg tccagccttc
    66301 cccgcccagc cccagcccgg caatgcacgg tccgttctta acgaaaatgc tggtatcaat
    66361 ggcgttcgcc atctggttca tgttttcgat attgcgcgag tgcattgccg ccgtgtggtg
    66421 gcaaccgccc tccagtttca ccgccagcgc aatggcatcc gccacgttgg cgacgcgcac
    66481 gacgggcagc accggcatca tcagttcggt cacggcaaac ggatgttctg cagtggtttc
    66541 cacaaacagc aaacgcgttt cttgcggcac gttcaggccg attgccgccg cgattttcgc
    66601 cgcatcgcga ccaacccagt cacggctgac ggtgcctttt ccgcgctcgt cgatattttt
    66661 cagcaacacc ggttgcagct gctgcgcctg ttctgcggtc agtttcaccg cgtgctggcc
    66721 ttccatcaga cgcatcagtt catcggctac gctatcaaca acaatcagta ccttttcgtc
    66781 ggcacaaatg atgttgttat cgaaagaagc gcctttgacg atggactgag cggcacgggc
    66841 gaggtcggcg gtttcatcca ccactaccgg cgggttgcca gcgcctgcgg caatcagacg
    66901 tttattggtg tgtttacgcg ccgcttctac taccgcttcg ccgccggtta ccaccagcag
    66961 gccgataccc ggaaacttga acaagcgttg cgcggtttcg atatccggat ttgccacagt
    67021 aaccagtaag ttttccggcc cacctgcggc aacaatcgcc tggttgagca gcgtaatcgc
    67081 ccgctgggag acttttttcg ccgccggatg cggggcaaaa atgacgctgt tgcccgcggc
    67141 aatcaggctg atggcgttgt taattacggt tgccgccggg ttagtggaag gcgtcaccga
    67201 agccaccacg ccccagggtg cgttttcaat tagggtcagg ccgttgtcgc cagtcagcac
    67261 ttgcggagag aggcactcaa cgcctggtgt gccgcgcgcc tgagcgacgt tttttgcaaa
    67321 tttatcttca acgcgcccca tgccggtttc actgacggca agttccgcta aatctctggc
    67381 gtgtttttcg cctgcttcac gaatggcagc aatggctaac tggcgcattg ccacgctttt
    67441 taacccttgc tgggcgactt tggctgccgc aacggcgtca tccagggacg cgaaaacgcc
    67501 catctcatga acggcggcgg acggcgtgtc actgctttgc attttcagca gtaccgcttt
    67561 caccacctgt tcaatatcct gttgattcat gatgttctgc cttatttgtg gaaaattacc
    67621 tgaccgccag acaccacctc atcgacaatg ccaatcacgc acagatcgac cggtgacgtt
    67681 tcgcttttat gcgcctggcg ggcggaactg ccactcacca gtaacaccca ctccccggtt
    67741 cccgcgccaa tattgtcgat ggcgacggcg cattgcccgt caggattacc ttgtggatca
    67801 atcatttcca ccatcagcaa tttgtcatgc gccagtccgt gatggcgtac ggtacaaaca
    67861 atttgtccag tgacgactgc cagtttcata cccgcctccg tggcgtattt caggtaaaag
    67921 ctccccctac cctccgcaga aggtaaaatg aaaaaggaga gagcgtgacg cccgaatcga
    67981 cgtcacacag ggtgattaca ggttgctgct atcgcctttc aggccgatcg ggaagacttc
    68041 ttccaggtcg ccgtgcggac gcgggataac gtgtacagat accagctcgc cgatacgctg
    68101 tgccgctgcg gcacctgcat cggtcgcggc tttacaggct gcaacatcac cacgaaccat
    68161 ggcagtacac aggccgccgc caatctgctt aacaccaacc agcttgacgc gcgcagcttt
    68221 caccatcgcg tcagaagcct caatcagtgc aaccaggccc cgggtttcga tcattcctaa
    68281 tgcttccatt gtgctttcct ctttatcagg gtccagaacg ggaccgttca ttcaaccagt
    68341 gtttgtaaac tgctttcgcg gttcacttct gtctgacgcg gcacagctgc caccagcgcc
    68401 agctcgataa tttcctgcac gctacaacca cgagagagat cgtgcatcgg cgcggcaagt
    68461 ccttgtatca gtggcccgac ggcacgatat ccgccgagtc gttgtgcgat tttgtaacca
    68521 atatttccgg cttccagcga cggaaaaacc atcacattgg ccttgccctg tagcgggctg
    68581 gcaggcgctt tttgcgccgc cacttccggc acgaaggcgg cgtcaaactg taactcgcca
    68641 tccaccacca gctttggtgc gcgctcacgg acgatttctg tcgcctgctg gacgttagca
    68701 acacaggggt gacgggcgct accattgctg gaaaacgaca gcatcgccac gcgcggctct
    68761 tctccggtga tggcgcgcca ggtttcggca ctggcaagcg cgatatccgc cagctgcgcc
    68821 gccgtcggct gtggcaccac gctgcaatcg gcaaagccca acgccgggcc gctgtactgt
    68881 ggcaacatca ggaaaatgga agagagcgtt ttacagcctg gctgcaagcc gataatgcgt
    68941 aatccggcac gcagcacatt tgccgtggaa gagaggttgc ccgcgataca gacatccgct
    69001 ttaccggcgc tgaccattgc tgcggcgaac atcagagggt cggtaagttt ttccagcgca
    69061 tccggcggcg ttttttcgcc cgcgcgggcc agccagcgat gagcaaattc ttcccgcatt
    69121 gcgaggttgc catgcggatc aatcacctgt agcccgtcca tcgccacgcc gtgactgagc
    69181 gcaaactgac gaagttcaaa cggattggcg accagaatgg gcgttgccag accttgttga
    69241 tgtaaatatt gcgcggcttt cagcacacgt tggtctaacg catccggaaa aaccactctg
    69301 gcgggcgctc gcaacgccag ttcacgacaa cgttcaataa tcattgcgtt tcccccagtc
    69361 gttgttggat ctgcttcacc gttagcggct gcttcgttac gctcaggatc atcatcacgt
    69421 agaccgtgct ggaaaggcgg ttcagcgcct gcaaaatgtc cgggcgtaat acttcaaagc
    69481 tgcgggtgat aaacacctgc gccgctacgg tttcggtttc ccgcactttg gtacgtagca
    69541 gattgagcaa cgccgcatcg cgcccgtggc tggcttccgg caccagatga tcgtggtcga
    69601 gatagcgcag cggctggtga gaaagtcgat gcagatcttc gtcagaaagc ccgacaatcg
    69661 cctggcagcc cagcggttca cccagcgcat cggcgcgcat aatgttgcca agacgcgaac
    69721 ggatatccgc cagccacggc tgccacggtt ccgccagttc aatttgcagc cacactgcca
    69781 gcgcaatggt gctgtcgaga accgcgcgaa aacccagacg cggatcgctt ttggcgacca
    69841 ttttttccgc cgacaggtga gtcagcgtat ccggcttttt tgccaccggc tggcggcaca
    69901 gttcgcagca cgcctgcgga tgttcatcgc tactggtcag cccatgaacg ggctgcggct
    69961 gctgctgttc atcgtcaaca aacaggcggc cctgctcgtc aataaacttg atgcgcagat
    70021 ggcggctttc cagtaactcc cgggcagagg gcgtcaggcg actgtccgca gggagatgga
    70081 tctctgcgcc ttcgctgagc gtatggttcg ctcttagcca tgcttcggtg atgaaatctt
    70141 tcatagggat tgccagttag ccggccaggc gacatacagg aatttcacgc tggatgtcgt
    70201 accaaattcg atactggagc ctttcgggat aaacatcacg tcacccgctt tggcgatcat
    70261 ggtttcgcct tcgtggcgaa catgcagctc cccttccagc accatgtcga tttcgtcgta
    70321 gttcagggtc cacgggaaga atgcgttctc ccactgcata aaaccagcgg ccatgctgct
    70381 gccgtcgtct ccggtcacaa gatcagtcaa gcccacgcaa tgcggctctg cgccgtcgaa
    70441 acgaccaaac ttgacgctgc tgccatcaat cactttgatg ccgcctttgc cggtcaccga
    70501 tttgaagctc ggctgcattg cgccctgctc cagcgactgt ttttccttca tcactttttc
    70561 catcagctgt gcgaccaggc tttcggtaaa ctggccttcc ggcagctggg caatgatggt
    70621 ttcgcggatg cgctggcttt cggttttgtc ggctggcacg ctggcgggca cggacgccgt
    70681 taccgggatc gattcatcac attcggtgat ggtaaaaccc agtagatccg ccacttcacg
    70741 ggcttccggg gtaataatgc tggcgcgcag aaccacggac attgcctgtt cgccgcgcgc
    70801 gtgtgcttca cgaatatcat tcgctgtgat gagttttttc acctgcttcc tcctgtttgg
    70861 tgagagatgc cagataatcc accagttgct gcacgctttg cgggtcgtgg ctgtttagct
    70921 cgaaaatcgg ctcttcgaaa cccgtctcaa gcaataactt tcgcgttgcg gcgacgtcag
    70981 catctggcat gtccgtttta ctgatgacgg cgatttgccg tttgctgacg ccaatatcca
    71041 gcaacccggc aggcaaccga ctttccgggt catttgcgcc atggacatag atcagcatgt
    71101 ctacatcctg tagcgtggta attaaggcgt gataccagcg gggatggcta aaatattcac
    71161 ccggcgtatc aatatcgccc ttatcattaa attccacggc ctgtgttttt ctggcgaggg
    71221 tataattccc ctgcaacgca ttaaataacg ttgtttttcc tgccccgacg gtacccacaa
    71281 acgcaattcg tttcatgaat gcctcgtatt aacttttggt catttcacaa agcgtataat
    71341 ttaataagcg acctaaaccg ctgacggttt gtgataacgc ttcttctact gcgcccaccg
    71401 agccataaat caccaatgca ccgctgaagc ggtcgagaaa accgatatgc acatcagcgg
    71461 ctttcagcgc taaatcaccg gcgatcatcg ccgtttcgcc gggagttagc gtcataatgc
    71521 caattgcgcc cgcatccgga acgccgattt tcttcgccag ttcctcaccg ggatgcgcga
    71581 tgagatgcgc cagtgtgacc tgtttgccgg gcacaaattc ctgaatgatg cgttctttat
    71641 ccatgagtcg ccgcctgtga aaaactttcg tgtatcgtga caatttttcg tcagagtaaa
    71701 taacaaaatt cggcaagcgc gtttaaaagg tgaggtagat cactaagaat ggagagatat
    71761 tcgctgtggt gcataaactt tcatatgcaa cgcatgaata taaataaaat aatatcgaga
    71821 tattatatag actgcttttt ttattattcg caaactcagt aataagcttt actaatatat
    71881 tgcagccaaa ccgcttaccc tgaatattca gggtaagcgt gagagttaaa aaagaattac
    71941 agcggttggg tttgcgcttc aaccacggcc agcgccacca tgttgacgat acgacgcacc
    72001 gatgcgatcg gcgttaacac gtgaaccggt ttcgcaacgc ccatcagcac cggaccgaca
    72061 gtcacacctt ccgagctgga aacgcgcagt aagttgtaac taatgcgggc agcttccatg
    72121 ttcggcatca ccagaatatt ggcagaacct ttcaaagggc tgtccggcat acggtcgttg
    72181 cgaatcgctt ccaccagcgc tgcatcgccg tgcatttcac catcaatcat cagatctggc
    72241 gcacgatccc tgaccagttc cagcgcctga cgcattttgc tcgacgacgg gcagtcagaa
    72301 gaaccaaagt tggagtgcga caacagagca acgcgcggct caataccaaa acgacggaca
    72361 gtttctgccg ccatcaaggt gatctccgcc agctcttctg cgtctggttc gtcattgacg
    72421 taggtatcgg caataaaggt gttaccactc ggcagcagca gcgcgttcat tgcacctgcg
    72481 gtgtgaacgc catcgcgata accaaagaca tttttcacca cgctaaaatg ttcatgataa
    72541 tcgcccaccg taccgcagat cattgcatcg gcttccccac gctgaaccat gatcgcgccg
    72601 atcactgtcg ggttactgat cagcgcccgc tgcgcctgtt cctgagtgac accgcgacgc
    72661 ttcatgatct ggaagtattc ggtccagtac tctttaaagc gcggatcgga ttcgttattg
    72721 acgatctcaa aatcaacgcc cgctttgatc tgcaagccca gtttctgaat gcgcatttcg
    72781 atcacgttcg gacgaccgat aaggatcggt ttcgccagtc ccagcgttac cagttcctga
    72841 gtggcatgca gaacgcgcgc ctcttcccct tctggcagaa caacgcgctt cggcgctttg
    72901 cgagcctggg agaaaatcgg cttcataaac aggttggttt tgtaaacgaa ctcagtcagc
    72961 ttgtcgatgt agacgtcgaa atcagcaatc ggacgcgtcg ccacgcccga ctccatcgcg
    73021 gctttagcga ccgcaggagc gatcttaacg atcaagcgcg gatcaaacgg ttttggaatg
    73081 atgtattccg gaccaaagct cagatcctga tcgccatacg ctgaagccac cacttcgctc
    73141 tgttcggcgt gggccagttc cgcaatcgcg cgtaccgccg ccagtttcat ctcttcattg
    73201 atagccgttg cgccaacatc cagcgcgcca cggaagataa acgggaagca aaggacgttg
    73261 ttgacctggt ttggatagtc agaacgaccg gtgcaaatga tggcatccgg acgcacttct
    73321 ttcgccagcg gcggcagaat ttccggttcc gggttcgcca gcgccaggat cattggcgca
    73381 cgagccattt tcttcaccat ttcctgggtc agcactttcg ggccggaaca tccaaggaaa
    73441 atatccgcgc cttcaatcac atcatcgagg gtacgtttgc cgtcatccac caccgcatat
    73501 gcggctttgg tttccgccat gtttggctca cgcccctgat agataacgcc ttttgaatcg
    73561 caaaccacga tgttatgttt ttgcagaccc agcgctacca gcaggttcat acaggcgatt
    73621 gctgcggcac ccgcgccgga aaccaccatc cgcacgtcgg agatgttttt ctccaccacg
    73681 cgcaagccgt tgaggatggc ggcagtgctg atgattgccg tgccgtgctg atcgtcgtgg
    73741 aataccggaa tattcatccg ctcgcgcagt ttctgttcaa tatagaaaca ttctggcgct
    73801 ttaatgtctt cgaggttgat tccgccgaag gtcggttcga gcgcagcgac aacttcaata
    73861 aatttgtccg ggtcgagttc atcaacttca atgtcaaata catcaatccc ggcgaatttc
    73921 ttaaacagaa cgcccttgcc ttccatcacc ggtttgccag ccagcgcgcc aatgttgcct
    73981 aaccccagca ccgccgtacc gttagagatc accgccacca ggttaccgcg cgcggtatat
    74041 ttgtaggctt ttaacgggtc tttttcgatt tcaagacaag gtgcggcaac gcctggtgag
    74101 taggccagcg ccagatcgcg ctgcgttgcc agaggcttgg ttggagaaac ctggattttc
    74161 cctggaaccg gaaattcatg gaaatcaagt gcactttgtt ttaactggtc atccatttgg
    74221 ttgttccttt cacgtaacgt tcacaaataa agtgtgtggg caacagcccc tgcccacaac
    74281 gtggcgcaca ttattaccct gccggagtct acagactttg agcaagtcca aactctcacc
    74341 attaatataa tgttttggta ataatcctat aacactgatg ttacctgctt aatccagcaa
    74401 taccatgcct gtctgctatg cttttttgat gcgtttagcg aaatttctca gaagtgtgaa
    74461 ttaacgcact catctaacac tttacttttc aaggagtatt tcctatgaac gagttagacg
    74521 gcatcaaaca gttcaccact gtcgtggcag acagcggcga tattgagtcc attcgccatt
    74581 atcatcccca ggatgccacc accaatcctt cgctgttact caaagctgcc ggattatcac
    74641 aatatgagca tttaatagac gatgctatcg cctggggtaa aaaaaatggc aagacccagg
    74701 aacaacaggt ggtcgcagcg tgtgacaaac tggcagtcaa tttcggtgct gaaattctca
    74761 aaatcgtacc cggtcgcgtg tcaacagaag ttgatgcacg cctctctttt gataaagaaa
    74821 agagtattga aaaggcgcgc catctggtgg acttgtatca gcaacaaggc gttgagaaat
    74881 cacgcatttt gatcaaactg gcttcgacct gggaaggaat tcgcgcggcc gaagagctgg
    74941 aaaaagaagg tattaactgc aacctgacgc tgctgttttc ttttgcccag gcgcgggcct
    75001 gtgcagaagc gggcgttttt ctgatttcgc cgtttgtcgg gcgtatttat gactggtatc
    75061 aggcacgcaa gccgatggac ccgtatgtgg tggaagaaga tccgggcgtt aaatcggtgc
    75121 gcaatatcta cgactactat aagcaacacc actatgaaac cattgtgatg ggcgcgagct
    75181 tccgtcgcac cgaacaaatc ctcgccttaa ccggctgcga tcgactgact atcgcaccga
    75241 atttgctgaa ggagctgcag gaaaaagttt cgccagtggt acgtaaatta atcccgcctt
    75301 ctcagacgtt cccacgccca gcacccatga gcgaagcgga gttccgttgg gagcacaatc
    75361 aggatgcgat ggcggtagaa aaactgtctg aaggcattcg tctgttcgct gttgatcaac
    75421 gcaaactgga agatcttctt gccgccaaac tataaaccag ccacggagtg ttatatgtcc
    75481 cgaaaagacc ttgccaatgc gattcgcgca ctcagtatgg atgcggtaca aaaagccaat
    75541 tctggtcatc ccggcgcgcc gatgggcatg gctgatattg ccgaagtgct gtggaacgat
    75601 tttcttaaac ataatcctac cgacccaacc tggtatgatc gcgaccgctt tattctttcc
    75661 aacggtcacg cgtcgatgct gctctacagt ttgctacatc tgaccggtta cgacctgccg
    75721 ctggaagaac tgaagaactt ccgtcagttg cattcgaaaa ccccaggcca cccggagatt
    75781 ggctatacgc caggcgttga aaccaccacc ggcccgcttg gacaaggttt ggcgaacgcc
    75841 gtcgggctgg cgatagcaga gcgtacactg gcggcgcagt ttaaccagcc agaccatgag
    75901 atcgtcgatc acttcaccta tgtgtttatg ggcgacggct gcctgatgga aggtatttcc
    75961 cacgaagtct gttcgctggc aggcacgctg ggactgggca agctgattgg tttttacgat
    76021 cacaacggta tttccatcga cggtgaaaca gaaggctggt ttaccgacga tacggcaaaa
    76081 cgttttgaag cctatcactg gcatgtgatc catgaaatcg acggtcacga tccgcaggcg
    76141 gtgaaggaag cgatccttga agcgcaaagc atgaaagata agccgtcgct gattatctgc
    76201 cgtacggtga ttggctttgg ttcgccgaat aaagcaggta aggaagaggc gcacggcgca
    76261 ccactggggg aagaagaagt ggcgctggca cggcaaaaac tgggctggca ccatccgcca
    76321 tttgagatcc ctaaaaagat ttatcacgcc tgggatgccc gcgaaaaagg cgaaaaagcg
    76381 cagcagagct ggaatgagaa gtttgccgcc tataaaaagg ctcatccgca actggcagaa
    76441 gagtttaccc gtcggatgag cggtggttta ccgaaggact gggagaaaac gactcagaaa
    76501 tatatcaatg agttgcaggc gaatccggcg aaaatcgcca cccgtaaggc ttcgcaaaat
    76561 acgcttaacg cttacggacc gatgctaccg gagctgctcg gaggttcagc ggatctggct
    76621 cccagcaacc tcaccatctg gaaaggctct gtgtcgctga aggaagatcc ggcgggcaac
    76681 tacattcact acggggtgcg tgaatttggc atgaccgcta tcgccaacgg cattgcgcac
    76741 cacggcggct ttgtgccgta taccgcgacg ttcctgatgt ttgttgaata cgcccgtaat
    76801 gccgcgcgga tggcggcact gatgaaagca cggcagatta tggtttatac ccacgattct
    76861 attggcttag gggaagatgg tccgacgcac caggccgttg agcaactggc cagtctgcgc
    76921 ttaacgccga atttcagcac ctggcgaccg tgcgatcagg tggaagcggc ggtgggctgg
    76981 aagctggcgg ttgagcgcca caacgggccg acagcactga tcctctcaag gcagaatctg
    77041 gcccaggtgg aacgtacgcc ggatcaggtt aaagagattg ctcgtggcgg ttatgtgctg
    77101 aaagacagcg gcggtaagcc agatattatt ctgattgcca ccggttcaga gatggaaatt
    77161 accctgcaag cggcagagaa attagcagga gaaggtcgca atgtacgcgt agtttccctg
    77221 ccctcgaccg atattttcga cgcccaggat gaggaatatc gggagtcggt gttgccttct
    77281 aacgttgcgg ctcgcgtggc ggtggaagca ggtattgccg attactggta caagtatgtt
    77341 ggtctgaaag gggtaattgt cgggatgacg ggttacgggg aatctgctcc ggcggataag
    77401 ctgttcccgt tctttggctt taccgccgag aatattgtgg caaaagcgca taaggtgctg
    77461 ggagtgaaag gtgcctgatg gtgatcgccg gatgctgatt gccggatgcg acgctgacgc
    77521 gtcttatccg gcctacagcg tcttatccgg cctacatgtc cccgcaattt tgtttaacgg
    77581 gtgatccaca acgtgggcca ggcgtctggc ccatgccagt tatcgcaggt gggttctgcg
    77641 gcataacgca ccaggcgaaa acgctgaccg tcaaagcgcc agcgcgcctg aatgccacaa
    77701 tcgcttaatc cgcgcccttt cgctaaggtc accagttcac gcgatttctc atcaaatgtt
    77761 gcgttcatca gttccagttc attcgtctcc tgaccgttgt tgaatggcaa acgcaaccga
    77821 accgggcgcg aggctagtgg ctttttacgc gacacaatcc atgccaaatc aatggtgtta
    77881 taggcccctg cctcacagct aatcatcatc agcgctttat catcagtcag cgcagtgaca
    77941 ttcacctcac gacgtaacgg atcaagcgag cagcgcagac cattcatccg ccagtttcca
    78001 taatccagca aatcgttgcg ttcttcgagt gagagtggcg tcggcgttgg gttaaccacc
    78061 gcgacctctt tcagcgcagg cgcgggcggt acgctgagcg gcggttcgtc ccctttcttg
    78121 atccacgcgg tttcactgcc aacgcgcttt tgctgagcat caataaacaa caacgccgct
    78181 tttaagccac tcagagaaat ggtctgattg ccgtcgcgta aggtgatcgc cttcccttcc
    78241 tgaatcacct gcaaaaacgc ggtgatggtt gccgtatcat cggttactaa taaccatggt
    78301 gaaatccgcc acttgtcacc acttaacgcc aacggctcac catctaacag cagccgtggc
    78361 gctatctctc cttcagacgc atccggcgac ttcaacccgc cgcgctcaat acgtaaaacg
    78421 gcatcggtat gcgccccggc gctgcggctc agggtcatca ccagtccatt atgatcgccc
    78481 gtattgcgcg ccacgcagaa gttttggtta ttacaggtga cctgccagtc ggaaaacgcc
    78541 cgttgcgctg gtgccgccca caccaaagac gtcggcaaca aagcgaaaaa aaagagaagg
    78601 aaaatgcgat agcgcatgga cggtacgacc ccagaagttc aaacaaacag cacaagtcgt
    78661 atcttcgttg cgcaggtagc gcggctcaat cagatttgtc ggattgagcc aataataaac
    78721 aacgaaatat tattgttatt tttcagtcca ttaaatgtga cgtttgcaaa tagttaagca
    78781 ataacaccgt cttaccgtca cgtatctcgc cggttttgat catctccagc gcctggctga
    78841 acggcagctc gagcacttca atatcttcat cttcaacacc gccaccggcg ttagcgcgct
    78901 gattgtcact gtattcggcg ataaaaaagt ggattagctc agtcacaccg ccaggcgaca
    78961 tatacagttc aaataatttg cgcacttcgc ccacttcata gcccgtctct tcaatcgctt
    79021 ctttacgaat gcacacttcc ggttcgtcgt tatccagcag cccggcgcag gtttcaatca
    79081 gctgcccgct ttcattgcca ttaacccagg tagcgacacg gaactgacga atcagaacca
    79141 cgctcttttt cttcgcgttg tacaggagga tcgtcgcacc attaccgcga tcgtaaactt
    79201 cacgtttatg gcggataact tcgccatctt tgcgcgtgag atcgtaggta atgttgtgca
    79261 gggtgaaata gttatcggag agaattttgt ctttaatgag ggtgatttgt tgcgtcatac
    79321 cgactccaca gcgcgaaatg aacaattatc ttacgctgtg aagtcgggtt tgtctgcgca
    79381 ggctatcagt gagatttgac tgattttacc cccagccagt caataatccc ctgtgccgca
    79441 tgacgtcctt ctgccattgc ggtaaccacc agatccgcac cacgcacggc gtcgccaccg
    79501 gcgaagattt tcggattggt ggtctggtaa cggtactggc tttccacatc cgcgatgatg
    79561 cggccccatt tgtctaccgt tacaccgtgc gactccagcc acggcatccc gtgtggattg
    79621 aagccaaacg ccataatcac cgcatcggct ggcatgacaa attcactgcc ctcaatcggt
    79681 acaggtcgcc gacgcccctg ggcatccggc tcgccaagac gcgtgcgcag gaaacgaatc
    79741 ccgcagacgt gaccttgttc attcagctca agcgccaccg gctggacgtt aaattcgaag
    79801 ttggccccct cttcgcgggc gttcctcacc tctttcttcg agcctggcat gttagcttca
    79861 tcacgacgat aagcgcaggt gacgttactc gcgccgtggc gcagcgcagt acgcacacag
    79921 tccatcgcgg tgtcgccgcc gcccagtacc acgacgttaa gtccggcggt attgataaac
    79981 ggctcttccg gtagctcttc gagccccatc acctgtttag tgttggcaat gaggaacggc
    80041 agcgcgtcat aaacgcccgg cgcatcttca ttgggtaaac ccgcgttcat ggaacggtaa
    80101 gtgcctacgc caacgaagac cgcgtcgtat tgttccaaaa gcgaatccaa agagacatct
    80161 ttacccactt cacaattgag ttcgaagtga atccccatcg cgctgaagat ttcccgacgg
    80221 cgtgccagca gggatttatc cagtttgaaa gaaggaatgc cgaaagtgag caagccaccg
    80281 atttctggat ggcgatcgta caccgtcacc gccacgccat tgcgggtcag aacatccgca
    80341 caggccagcc ctgccggacc tgccccgata atcgccaccc gcttgtccac tttggtgaca
    80401 tgacttaagt caggacgcca acctttcgcc aacgcctgat ctgaaatata gcgttcaata
    80461 ttgccgatag ttaccgcgcc gtgctcatcg cgaatagtac aggcaccttc acacaaacgg
    80521 tcttgcggac aaacgcgtcc ggtaatttcc ggcagggtgt tggtctggtg agaaagctcg
    80581 acggcggcgt cgatgtttcc ggctttcacc agttcaatcc actgcggaat atggttatgc
    80641 agcgggcagg tccattcaca aacgctgtgc tcgccgcact taaggcagcg cgaggcttcc
    80701 cgttgtgcct ggtcggcgcg aaatggcaga taaatttcat caaaaccggt tttgcgcgct
    80761 tcaatcgcca gtttatccgg ctcgccacgc gcgggcgttg cctgcatttg ttcgacttta
    80821 ctcattaccg gcatttcttg cgccgcggta ctggcatgcc acggttgatg ttcctgacgc
    80881 gcggtgcgca agcggcgggg tttcgccatg ccggagagtg cgacgtcagt gaccagttgc
    80941 agcgcgtccg ccgggcaatt ctcaacacag gcaggaccgt tttcgcgccc cgcacaaagg
    81001 tcgcatttat gcgccgtggc ttttactttt cctgccgcga cgggtgtcag gacgatttgc
    81061 atcgtaccaa aaggacaggc caccacgcag gatttacagc caatacactt ttgctgattg
    81121 acctgaatgc tgtcatcaac gtggctgatt gcgccattag ggcagctacg ggcgcagggc
    81181 gcatcttcac aatggtgaca ggtcactgca ctacgttgct gttgatgttt gataaccgta
    81241 attcggggat gaaaatggtg ttggctcagg acatgttgct catcattgtg agccatgaca
    81301 caggcgattt cacaagcatg acaacccaga cattgctgac tgttggccat aataaaacga
    81361 ttcataacga ccttcttttt tggttgtaaa aaccttattc tttatatgag tgttgttatt
    81421 acccgactta caggggaatc ggcaatgttc atgtgcccag aataagtaac tatttcgcta
    81481 taaactgtgg cagatcaaat aatcccatca ctgactaaat tgtgtttcag ctaactggaa
    81541 cattaatgat tttttgtgga gaagacgcgt gtgattgtta aacgacccgt ctcggccagt
    81601 ctggcccggg ccttttttta cattgtgctg ctgtcgattc tttccacggg tatcgctctg
    81661 ctaactctgg cgagcagttt gcgcgacgct gaggctatca atattgccgg atcgctacgt
    81721 atgcagagtt accgcctggg ctacgacttg caaagtggca gtccacaact caatgcacat
    81781 cgccagttgt ttcagcaggc actgcattca ccggtattaa ccaatctcaa cgtctggtat
    81841 gtgccagaag cagtaaaaac tcgctatgcg catctgaatg ccaactggct ggagatgaat
    81901 aatcggctca gcaagggcga tttgccgtgg tatcaggcca atattaataa ttatgttaat
    81961 cagatagacc tgttcgtgct ggctttacag cactacgctg aacgcaaaat gctgctggtg
    82021 gtggcgattt ccctggctgg cggtatcggt attttcacgc tggtcttttt tactttgcgc
    82081 cgtatacgcc atcaggtggt tgccccgctg aatcagctgg ttaccgccag tcaacgtatt
    82141 gaacacgggc agttcgactc gccgccgctg gataccagtc tgccgaatga gcttggtctg
    82201 cttgcaaaaa cctttaacca gatgtcgagc gagctgcata aattgtacct ttcactggag
    82261 gcgtcagtag aagaaaagac ccgcgatctc cacgaggcca agcgtcgtct ggaggtgttg
    82321 tatcaatgtt cccaggcgct gaacaccagc cagattgatg tgcattgttt ccgccatatt
    82381 ttgcagattg ttcgcgacaa tgaagcggct gaatatctgg agttaaatgt cggtgaaaac
    82441 tggcggatta gcgaagggca accaaacccg gaattgccga tgcagatttt accggtgaca
    82501 atgcaagaga cggtttacgg cgaactgcac tggcaaaata gtcacgtttc atcatcagaa
    82561 ccgctgctta acagtgtttc atcgatgctg ggacgcggtt tgtactttaa tcaggcgcag
    82621 aagcattttc agcagttatt gttgatggaa gaacgtgcaa ccatcgcccg cgaattgcac
    82681 gactcgctgg ctcaggtact ttcttactta cgtatccagt tgacgttact gaagcgttcg
    82741 ataccggaag ataacgccac cgcacaaagt atcatggccg atttttccca ggcgttgaat
    82801 gatgcttatc ggcagttacg cgagctgttg actactttcc gcctgacgct gcagcaggcg
    82861 gatctcccct ccgcgttgag ggaaatgctg gatacgttac aaaatcaaac cagcgccaaa
    82921 ctgaccctcg actgccgtct gccaaccctg gcgctggatg cgcaaatgca ggtgcatttg
    82981 ttgcaaatta ttcgcgaagc ggtgctgaat gcgatgaagc acgccaacgc cagcgaaatc
    83041 gccgtcagtt gcgtcaccgc gccggacggc aatcacacgg tttatatccg tgataacggg
    83101 attggtatcg gtgaaccgaa agaacccgaa ggtcattatg gtctgaatat catgcgcgaa
    83161 cgcgcggaac ggctgggtgg gacgctgact ttttcacaac cttccggcgg cggcacgtta
    83221 gtgagtatta gctttcgctc tgcggagggt gaggaaagtc agttgatgta atgcctccta
    83281 ctgaccaaag aatacttgca cttaaggttc agtataaaag ggcatgataa tttacattaa
    83341 ctcctttttt tctccacgat tggctcgtac cttgccgcta cagtgaagca agtcaagcct
    83401 acaacgatac gcagaaacac gaggtcctct tttaatggcg aatttcttta ttgatcgccc
    83461 catttttgcc tgggtgctgg caatcctgtt gtgtctgaca gggaccctgg cgattttttc
    83521 attgcccgtt gaacaatacc ccgatctcgc gccaccgaat gtgcgagtga ccgctaacta
    83581 tcccggcgca tcggcccaga cgctggaaaa caccgtgacc caggttatcg agcaaaatat
    83641 gaccggcctc gataatctca tgtatatgtc atctcagagc agtggcaccg gtcaggcatc
    83701 tgtcacttta agttttaaag caggcaccga tccggacgaa gccgtgcagc aagtacaaaa
    83761 ccagctgcaa tcagccatgc gaaagttacc gcaggcggtg caaaatcagg gcgtgacggt
    83821 gcgtaaaacc ggcgatacca atattctgac cattgctttc gtctctaccg atggttcgat
    83881 ggataaacag gatattgccg attatgttgc cagtaatatt caggacccgt taagccgcgt
    83941 gaatggcgtc ggggatatcg atgcctatgg ttcgcaatat tccatgcgta tctggctgga
    84001 cccggcgaaa ctcaacagtt tccagatgac ggctaaagat gtcactgatg ccattgagtc
    84061 acagaacgcg cagattgcgg ttgggcaact tggtggtaca ccttccgtcg ataagcaggc
    84121 gctcaacgcc accattaacg cccagtcact gctgcaaaca ccagaacagt tccgcgatat
    84181 caccttgcgg gtgaatcaag acggctcaga ggtaaggctg ggcgatgtcg ccaccgtcga
    84241 aatgggggcg gagaaatacg attatcttag ccgcttcaat ggtaagccag cctccgggct
    84301 gggggtaaaa ctggcctccg gcgcaaacga aatggcgaca gcggagctgg tgctcaatcg
    84361 tctcgacgag ctggcgcagt acttcccgca cggactggaa tacaaggtgg cgtatgaaac
    84421 cacctcgttt gttaaagcct ccattgaaga cgtggtgaaa acgctgctgg aagctatcgc
    84481 cctggttttc ctcgttatgt atctgttcct gcaaaacttc cgcgccacgc tgatacccac
    84541 tatcgccgtg ccggtggtgt tgatgggaac cttctccgta ctttacgcct tcggttacag
    84601 cgtcaacacc ttaaccatgt tcgcgatggt actggcgatc ggtctgctgg tggatgacgc
    84661 aatcgtggtg gtggaaaacg tcgaacgtat tatgagtgag gaaggactca ctcctcgcga
    84721 agccacacgt aaatcgatgg ggcagatcca gggggcactg gtcgggattg cgatggttct
    84781 ttcggcggta tttgtaccaa tggccttctt cggcggcacc accggtgcca tctatcgcca
    84841 gttctctatt accattgttg cggcgatggt gctgtcagta ctggtagcga tgatcctcac
    84901 tccggctctg tgtgccacac tacttaagcc actgaaaaaa ggtgagcatc acgggcaaaa
    84961 aggctttttt gcctggttta accagatgtt taaccgcaac gccgaacgct acgaaaaagg
    85021 ggtggcgaaa attctccacc gtagcctgcg ctggattgtg atttatgtcc tgctgcttgg
    85081 cggcatggtg ttcctgttcc tgcgtttgcc gacgtcgttc ttaccgctgg aagaccgtgg
    85141 catgtttact acctcggtac aattgcccag cggttcaacc caacaacaga ccctgaaagt
    85201 cgttgagcaa atcgagaaat attacttcac ccatgaaaaa gacaacatca tgtcggtgtt
    85261 tgccactgtt ggttctggcc ccgggggtaa cgggcaaaac gtggcgcgaa tgtttatccg
    85321 cctgaaagac tggagcgaac gcgacagtaa gaccggcacc tcgtttgcca ttatcgagcg
    85381 tgcaacgaag gcgtttaacc aaattaaaga agctcgcgtt atcgccagca gcccgccagc
    85441 aattagcggt cttggtagtt ctgcaggttt tgatatggag ttgcaggacc acgctggagc
    85501 gggtcacgat gcgctgatgg cagcacgtaa tcagttgctg gcgctggcgg cggaaaaccc
    85561 ggagctaacc cgtgtgcgcc ataacggcct cgacgacagt ccgcagttgc agattgatat
    85621 cgaccagcgt aaagctcagg cgctgggcgt tgctatcgac gatattaacg acacactgca
    85681 aaccgcctgg ggttcgagct atgtgaatga ctttatggat cgcggtcgcg tgaagaaagt
    85741 ctatgtgcag gcagctgcgc cgtatcgcat gctgccagat gacattaatc tctggtatgt
    85801 ccgaaataaa gatggcggca tggtgccctt ctctgctttc gcgacctcac gctgggaaac
    85861 aggctcgccg cgtctggaac gctataacgg ttattctgcg gttgagattg ttggggaagc
    85921 cgcaccgggg gtcagtaccg gtacggcgat ggatattatg gaatcgttag tgaagcagtt
    85981 gccaaacggc tttggtctgg agtggacggc gatgtcgtat caggagcgac tttccggcgc
    86041 gcaggctccg gcgctgtacg ccatttcctt gctggtggta ttcctgtgtc tggcggcgtt
    86101 gtatgaaagc tggtcggtgc cgttctcggt aatgctggtc gtgccgctgg gggttatcgg
    86161 cgcgctgctg gcaacctgga tgcgcgggct ggaaaacgac gtttacttcc aggtgggcct
    86221 gttaacggtc attggtttat cggcgaaaaa cgccatcctg atcgtcgagt ttgctaacga
    86281 gatgaaccaa aaaggccacg acctgtttga agcgacgctc cacgcctgcc gtcagcgttt
    86341 acgcccgatt ctgatgacct cgctggcatt tatcttcggc gtattgccaa tggcaaccag
    86401 cacgggtgcc ggttccggtg gtcagcatgc ggtgggtact ggcgtaatgg gcgggatgat
    86461 ttcagctact attctggcta tttacttcgt gccgctgttc tttgtgctgg tgcgccgccg
    86521 cttcccgctg aagccgcgcc cggaataagc aataaaaaag gcgacatgcc aatgtgtcgc
    86581 ctttttcaac tttccgatta agaacctgct cagcgggttc ttgctgttta tactttgtct
    86641 cgggaattac ttacgaagca taacttcgat aaagtctttc cagttcccca gttcacgttc
    86701 aatcataaca acctctctta taattatggg tattctacgg aaacaatata ccgtggtgaa
    86761 gctaatttac tcgattgctg cgatgactac ctccggggga caaaccttat gtaaatacta
    86821 tggtcctgcg gtgataattt gtatgtgata cacagcaaca tttcgagata ttcatacggc
    86881 atctaatact gatttaattc tggttaaaat acagacagat aacaagatga atat
//
LOCUS       DAZFWL020000005        70420 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFWL020000005 DAZFWL020000000
VERSION     DAZFWL020000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 70420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.005"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..857)
                     /locus_tag="ACHKNP_RS01905"
                     /old_locus_tag="pgaptmp_000381"
     CDS             complement(<1..857)
                     /locus_tag="ACHKNP_RS01905"
                     /old_locus_tag="pgaptmp_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU4702619.1"
                     /translation="MKQQVSFKFRLKPDGQQECQMRRFAGACRFVFNRALALQNENHE
                     AGKKYIPYTKMASWLVEWKKDTETEWLIDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEISTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHFRIANIRRDYLHKVTTIISKNHAMIVIEDLKVKYMSK
                     SAAGTISHPG"
     gene            957..3944
                     /locus_tag="ACHKNP_RS01910"
                     /old_locus_tag="pgaptmp_000382"
     CDS             957..3944
                     /locus_tag="ACHKNP_RS01910"
                     /old_locus_tag="pgaptmp_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702620.1"
                     /translation="MSLSDQVVMATSIETLIELLKNLPDYGRVSYVVTAKGDEVKTAF
                     DIVDASALLVSNTLDGKINPDYPQELQPRDRTRASSLLQVNQISKDLRPAQLTDSGLS
                     SHGAPIIGEDNAVESGNGRTMGIIKAYQDGNADRYREYLIEHATEFGIRPEKVESMTA
                     PVLVRRRLTTVDRVQFAKDSNISDLQEMAASEKAFVDADSITPAMMALFNPSESGDLL
                     SRSNDAFIRGFMTQVGATQAAGLVTEDGRPTRQLVDRIQNAIFAKAYKDARLVRMVAE
                     EPDPDMRNVLTALNAAANDFVQMQALSGEAHKQAVTTIVDGIETADSLDKKALAALKD
                     AVDLVRQSKESGQHITDVIAQGDMFSETAPEVKALALFIVANNRSAKRMATAFKLMAQ
                     RINDELQHQGQALGDMFGGGDVSLQDILRQVSQELENEGMQGISGGLFESASGGSYNG
                     VAPYTSLLLHRASGIKDIIHLIRLLSRTDPQDEQLVQVLAHFVRMPVADVKKWCRLFG
                     ISNSLLRGLLNHASSLGRDGFDEIAQAIKNGDMPPAIDWFSIRPTRVKAFLSAAHSAS
                     PLAEMVQRLSLIFTDHTALGDLTLDEMKEASIQWADQQNEVNSDFLPAFRKAVSKADD
                     ARGILKAFKELQSRVNKHVGDIDGVTAEGRDILKEHGITPEFIDEIRTDMQREVVSSL
                     QIVARALADANPKSAAIVNRVIGDIEASEGMGALKLFLSRAFNPNGNILPGIIGEAKK
                     YVSEEELEQLDQLLKRFSYNPQTRWQMNQRSMGSVHEKVLSAMNSAIANSSVSEEKAL
                     EWADSFITEEVEEARAGQNGGIDLRKELADIYRLTGGKISTLSKVVHHQGRAYANLNG
                     VVAVNLNDENASALWHELGHHLEYSNPGLLEKARSFLKANVEGDKPSFVNIGGRGKPE
                     WCFRSRLSNIYMAKVYPPASVSNTGKIRQKSPTISKTSATEVFSMALQLYHDKEAAAA
                     SLMNGDGLLELLLGVAKELNNAD"
     gene            3934..4242
                     /locus_tag="ACHKNP_RS01915"
                     /old_locus_tag="pgaptmp_000383"
     CDS             3934..4242
                     /locus_tag="ACHKNP_RS01915"
                     /old_locus_tag="pgaptmp_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702621.1"
                     /translation="MQIKIAAPLGGDAIIEFDDNEEVSGRLSIISGDITEDMIAEAIA
                     GANPNSYMGFVNTLDAPASDVLRTLHLYAGWFVDWPAVEGGDEDDDDDFGDHVDQIVY
                     "
     gene            complement(4274..5578)
                     /gene="yjjJ"
                     /locus_tag="ACHKNP_RS01920"
                     /old_locus_tag="pgaptmp_000384"
     CDS             complement(4274..5578)
                     /gene="yjjJ"
                     /locus_tag="ACHKNP_RS01920"
                     /old_locus_tag="pgaptmp_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013359111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family toxin
                     YjjJ"
                     /protein_id="HGU4702622.1"
                     /translation="MATHATTIRSLLNQGPMTPRQLVEKLSVSQPTISRALRELGDEI
                     VRIGNGRSIQYALRDSFRGFESVPIYRITEEGCICVLGTLIPVRPEGYVMVEDGKNNR
                     HSDGLPWWLFDMRPQGFLGRAYASAYALELNLPSNPEQWSDTDVIKALLKHGHDVVGN
                     LLIGEQARTHFLEMQPPVPVNREIEYPVLALAAGEGDVPGSSAGGEQPKFCTFTERGH
                     VLVKFSAPNDTPISERWRDLLFAEHLALKVLGVETEVFDYNGQRFLEIPRFDRVGQLG
                     RVGVFSLRALDAEFVGNARATWPLLVKELVKNKCVHPDAIIIATRLWSFGVLIGNTDM
                     HNGNLSFISSHGRPYHIAPAYDMLPMGFAPRSGGAMSNELRKTALPDFVSRKIWDESL
                     ELAEKFYALACEADKFSANFTPCLEALRIHLDDCKIRIARLA"
     gene            complement(5776..6564)
                     /locus_tag="ACHKNP_RS01925"
                     /old_locus_tag="pgaptmp_000385"
     CDS             complement(5776..6564)
                     /locus_tag="ACHKNP_RS01925"
                     /old_locus_tag="pgaptmp_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702623.1"
                     /translation="MAAQNTKTIQYRLRNGQSVEVTINNDGVPGEKVSISDLAIEKTI
                     MCHLGFTEEVSKKHGVAIWSAMDTGMRKFITARTPGMTMMDLMQIAPLFECEPLDVFS
                     NPAICQQLYGEVKLAVTPIVLHEGSLAGVWKVERISSYMPFHVNGVITGENQPVSVIK
                     SDLKRAILEASCRVIGLGKQSYVSFPAGPEGPAEILIMDADLLWQIQFLIGKSIIRAE
                     ELDQYITCTMTDEVKSVAIANARNLCRATLAEPQENIPEETESD"
     gene            complement(6767..7255)
                     /locus_tag="ACHKNP_RS01930"
                     /old_locus_tag="pgaptmp_000386"
     CDS             complement(6767..7255)
                     /locus_tag="ACHKNP_RS01930"
                     /old_locus_tag="pgaptmp_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4702624.1"
                     /translation="MAQRGVNKVILIGTLGQDPDIRYIPNGGAVGRLSIATNESWRDK
                     QTGQQKEQTEWHKVVLFGKIAEIASEYLRKGSQVYIEGKLKTRKWTDDAGVERYTTEI
                     IVSQGGTMQMIGARRDDSQSSNGWGQSNQPQNHQQYSGGSKPQSSANNEPPMDFEDDI
                     PF"
     gene            7425..7982
                     /locus_tag="ACHKNP_RS01935"
                     /old_locus_tag="pgaptmp_000387"
     CDS             7425..7982
                     /locus_tag="ACHKNP_RS01935"
                     /old_locus_tag="pgaptmp_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4702625.1"
                     /translation="MKGKTAAGGGAICAIAVMITIVMGNGNVRTNQAGLELIGNAEGC
                     RRDPYMCPAGVWTDGIGNTHGVTPGVRKTDQQIAADWEKNILIAERCINQHFRGKDMP
                     DNAFSAMTSAAFNMGCNSLRTYYSKARGMRVETSIHKWAQKGEWVNMCNHLPDFVNSN
                     GVPLRGLKIRREKERQLCLTGLVNE"
     gene            7975..8226
                     /locus_tag="ACHKNP_RS01940"
                     /old_locus_tag="pgaptmp_000388"
     CDS             7975..8226
                     /locus_tag="ACHKNP_RS01940"
                     /old_locus_tag="pgaptmp_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HGU4702626.1"
                     /translation="MNKLRQLRRLSTMKLSLAAIVFDSIFMAVYVLNETWPLEPLLYA
                     GLRLCLTFLSMAARLMQQKETASDCPRRAVRKYMARRRR"
     gene            8571..8792
                     /locus_tag="ACHKNP_RS01945"
                     /old_locus_tag="pgaptmp_000389"
     CDS             8571..8792
                     /locus_tag="ACHKNP_RS01945"
                     /old_locus_tag="pgaptmp_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4702627.1"
                     /translation="MVNANPCARPEFIWRFYSCKKHHYHFVIAATEDEARSQLPDGPC
                     IFTARFSTNSRNSLSYWNLPFSADVQGVL"
     gene            8789..9832
                     /locus_tag="ACHKNP_RS01950"
                     /old_locus_tag="pgaptmp_000390"
     CDS             8789..9832
                     /locus_tag="ACHKNP_RS01950"
                     /old_locus_tag="pgaptmp_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU4702628.1"
                     /translation="MKKPLVTRNEIAEAIALHTACMPTREIPGAIANYFMITRRFYTR
                     TDKAVINRLLIAEIRDYLIEQGRLRYATVAAEMRKEAHRMTSNNLNVKKPAPVASATS
                     APAVNVIPTTGDTIDSLTLLKMVNEARKLCGEPEVRNNKFIEKILDELEGEDGYTKSA
                     TVPPGGGTPMVVITMTYKQALRVAARESKAVRRSLIDKLEELQQANSPTPSIPQTLPE
                     ALRLAAELAEQKMQLEQQLVAAAPKVDFADRVSAANGILIGNFAKVVGLKQNALFSWL
                     RQNGILMAFGARKNVPRQQYINAGYFTVKEVVLDDENGYQIRLTPQLTGKGQQWLTRK
                     LLDAGLLKPVAAE"
     gene            complement(9908..10927)
                     /locus_tag="ACHKNP_RS01955"
                     /old_locus_tag="pgaptmp_000391"
     CDS             complement(9908..10927)
                     /locus_tag="ACHKNP_RS01955"
                     /old_locus_tag="pgaptmp_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head processing protein"
                     /protein_id="HGU4702629.1"
                     /translation="MTDVLKTVTDRFCLYSNARKGRQNGRQYVLSAVKTMLESKETQE
                     GLRLGELFGYYGHGRRQLTGKLEVPETSVIMVEGRPVVIDNVPACRTVAISVDDNGIV
                     THTQEILNTEPGKIVAAMIESRAGGWSWATGGRESGKIAVTTSFHGVDYVTTPNYISL
                     DHPASAGMFESADSKSLLAESLAAHGYSDESVQAVISHYGKMAELEMMVEATERTAEL
                     ETALLESQGRHLEAMAKIADAEARIALLEETAGIRNDVLAAMQDELDNLPIFVSAAQK
                     DAFRLKEPGDAKIVATLFESLIKVGARNLPVTKKIKEVPQAANVQAPRETSIITFNNS
                     INPFN"
     gene            complement(10920..12629)
                     /locus_tag="ACHKNP_RS01960"
                     /old_locus_tag="pgaptmp_000392"
     CDS             complement(10920..12629)
                     /locus_tag="ACHKNP_RS01960"
                     /old_locus_tag="pgaptmp_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4702630.1"
                     /translation="MADNKITLSSVRKALAGVFKDNGERDNILLSALAVHGGSGYLFS
                     RAGAPVQLSGFLGGKPGDSGMAGDGLVDGSRFIFDEVQLPEDRLQRYPLLEEMAVYST
                     IATALNIHITHALSFDKKTGQTFSIVPVHNGNDSDYDAAQGLCDELMNDIGRTINKEV
                     AGWAFIMSVFGVAYVRPYAKEGIGITSFECSYYTLPSFIKEFEVSGNLAGFSGDYLKD
                     ASGKMVFADPWTIIPMKTPYWRPKSNLMPVHTGHKAYSLLDNPEERTPIETQNYGTSL
                     LEYAYEPYMNLRSAIRSLKATRFNASKIDRIIGLAMNSLDPVKAADYSRTITQTLKRA
                     ADLMEKRARGANNMPTVTNTLLPIMGDGKGQMTIDTQTIQADINGIEDILTYMRQLAA
                     ALGLDYTLLGWADQMSGGLGEGGFLRTAIQAAMRASWIQQGVEEFIQRAIDIHLAFKY
                     GKVYPEGDRPYKIEFHSVNTALQQEHNDNRDSQANYATIVTQILDAVSNNSVLANSDA
                     FKRYLFSDVLEIDEKISEALVNELKAKSEDDDHLMDSIIKTPPQELAQILESVFKEGN
                     DND"
     gene            complement(13230..13871)
                     /locus_tag="ACHKNP_RS01965"
                     /old_locus_tag="pgaptmp_000393"
     CDS             complement(13230..13871)
                     /locus_tag="ACHKNP_RS01965"
                     /old_locus_tag="pgaptmp_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HGU4702631.1"
                     /translation="MDKKICVVSMSVGKPASMTAAWINNELIMAERTSYPERRRDMEL
                     QLLRELREKEEKGFIVLVEEENSFITGRVGQRVRLRDPFMNGRPVLIEAMQIYKELER
                     QKAIKLPRKESGKYILHQSIFDSEHDKKGDEFFNINWSEITTEHVLTLLCCFATEYNN
                     VASADYIRAMAGEVEARQEPSLLRPLINIIHGTQTLAQKQVPQGELTGKGNYL"
     gene            complement(14060..14620)
                     /locus_tag="ACHKNP_RS01970"
                     /old_locus_tag="pgaptmp_000394"
     CDS             complement(14060..14620)
                     /locus_tag="ACHKNP_RS01970"
                     /old_locus_tag="pgaptmp_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ref family protein"
                     /protein_id="HGU4702632.1"
                     /translation="MKTIEQKLEQRREWQKAARERAIARQREKLADPAWRESQYQKMR
                     DSIDRRIAKQKERPPASKTRKSAVKIKSRGLKGRTPTAEERRIANALGALPCIACYMH
                     GVISNEVSLHHIAGRTAPGCHKKQLPLCRWHHQHAAPAEVREKYPWLVPVHADGVVGG
                     KKEFTLLNKSEMELLADAYEMANIMH"
     gene            complement(14866..15177)
                     /locus_tag="ACHKNP_RS01975"
                     /old_locus_tag="pgaptmp_000395"
     CDS             complement(14866..15177)
                     /locus_tag="ACHKNP_RS01975"
                     /old_locus_tag="pgaptmp_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogeny establishment protein"
                     /protein_id="HGU4702633.1"
                     /translation="MRKDFKIDGKYVVLSVSSQIQSPSVIVTVKLSDRMPDIDSISVA
                     FPVKSMRSAEHFVMNATEEEARRGLTRVMAEFGELLGKVNNALSISSARSKALTASMM
                     K"
     gene            complement(15228..16259)
                     /locus_tag="ACHKNP_RS01980"
                     /old_locus_tag="pgaptmp_000396"
     CDS             complement(15228..16259)
                     /locus_tag="ACHKNP_RS01980"
                     /old_locus_tag="pgaptmp_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGU4702634.1"
                     /translation="MSNLLTVHQNLPALPVEATSDEVRKNLMDMFRDRQAFSEHTWKM
                     LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS
                     GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRN
                     LAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT
                     KLVERWISVSGVADDPNNYLFCRVRKNGVAAPSSTSQLSTRALEGIFEATHRLIYGAK
                     DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA
                     MVRLLEDGD"
     gene            complement(16267..16487)
                     /locus_tag="ACHKNP_RS01985"
                     /old_locus_tag="pgaptmp_000397"
                     /pseudo
     CDS             complement(16267..16487)
                     /locus_tag="ACHKNP_RS01985"
                     /old_locus_tag="pgaptmp_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697576.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
     gene            17092..17301
                     /locus_tag="ACHKNP_RS01990"
                     /old_locus_tag="pgaptmp_000398"
     CDS             17092..17301
                     /locus_tag="ACHKNP_RS01990"
                     /old_locus_tag="pgaptmp_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c1 repressor inactivator"
                     /protein_id="HGU4702635.1"
                     /translation="MAFIPPTIDDVRHCSNALSVDPAETDAARAIAEHYSKISNQEYR
                     ITQDDLDDLTDTIEYLMATNQPDSQ"
     gene            17412..18263
                     /locus_tag="ACHKNP_RS01995"
                     /old_locus_tag="pgaptmp_000399"
     CDS             17412..18263
                     /locus_tag="ACHKNP_RS01995"
                     /old_locus_tag="pgaptmp_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein C1"
                     /protein_id="HGU4702636.1"
                     /translation="MINYVYGEQLYQEFVSFRDLFLKKAVARAQHVDAASDGRPVRPV
                     VVLPFKETDSIQAEIDKWTLMARELEQYPDLNIPKTILYPVPNILRGVRKVTTYQTEA
                     VNSVNMTAGRIIHLIDKDIRIQKSAGINEHSAKYIENLEATKELMKQYPEDEKFRMRV
                     HGFSETMLRVHYISSSPNYNDGKSVSYHVPLCGVFICDETLRDGIIINGEFEKAKFSL
                     YDSIEPIICDRWPQAKIYRLADIENVKKQIAITREEKKVKSAASVTRSRKTKKGQPVN
                     DNPESAQ"
     gene            complement(18296..19414)
                     /locus_tag="ACHKNP_RS02000"
                     /old_locus_tag="pgaptmp_000400"
     CDS             complement(18296..19414)
                     /locus_tag="ACHKNP_RS02000"
                     /old_locus_tag="pgaptmp_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU4702637.1"
                     /translation="MRTSLVTREEMIEAIEQHTACISTRDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLSDIREYLLEQGHLNYATVAAEARKEAHRMKATNVKSEKIHAPSVQES
                     ELVVVQNQSDEIPVLEWQGVRVVTTETLARGYGTETIRIRQNHHENKVRFVEGKHFFK
                     VEGESLRELKHRVALNYSVKIARNVRSLTLWTERGAARHAKMLETDQAWAFFEKLEDS
                     YFRQKEQQPIAIPQTLPEALRLAAELAEQKQLLEQKAHQLNQQLVAAAPKVDFADRVS
                     VAKGILIGNFAKVVGLKQNALFVWLRENGILIASGGRKNVPFQQYINAGYFTVKEVVL
                     DDEDGYQIRLTPQLTGKGQQWLTRKLLDAGLLKPVAAE"
     gene            complement(19411..19632)
                     /locus_tag="ACHKNP_RS02005"
                     /old_locus_tag="pgaptmp_000401"
     CDS             complement(19411..19632)
                     /locus_tag="ACHKNP_RS02005"
                     /old_locus_tag="pgaptmp_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4702638.1"
                     /translation="MATIPTPTHPEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDSRNSLSYWCLPVNASAQEGL"
     gene            complement(19657..19974)
                     /locus_tag="ACHKNP_RS02010"
                     /old_locus_tag="pgaptmp_000402"
                     /pseudo
     CDS             complement(19657..19974)
                     /locus_tag="ACHKNP_RS02010"
                     /old_locus_tag="pgaptmp_000402"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            complement(19971..21455)
                     /locus_tag="ACHKNP_RS02015"
                     /old_locus_tag="pgaptmp_000403"
     CDS             complement(19971..21455)
                     /locus_tag="ACHKNP_RS02015"
                     /old_locus_tag="pgaptmp_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4702639.1"
                     /translation="MARSCVTDPRWRELVALYRYDWIAAADVLFGKTPTWQQDLIIES
                     VQEQGSKTSVSSGHGTGKSDMTSIMIMLFIIMYPGARAIIVANKIQQVMTGIFKYIKI
                     NWATATSRFPWLADYFVLTETAFYEVTGKGVWTVVPKGFRLGSEEALAGEHADHLLYI
                     IDEASGVSDRAFGIITGALTGQDNRILLLSQPTRPSGYFYDTHHKLAKRPGNPDGVYT
                     AITLNSEESPLVTPAFIKMKLAEYGGRDNPMYMIKVRGLFPKSQDGFLLGRDEVERAT
                     RRKVKIAKGWGWLACVDVAGGTGRDKSVINIMMVSGQRNKRRVINYRMLEYTDVTETQ
                     LAAKIFAECNPERFPNITIAIDGDGLGKATADLMYEYYGITVQRIRWGKKMHSREDKS
                     LYFDKRAYANVQAAEAVKSGRMRLDKGNETIEEASKIPVGINSAGQWKVMSKEDMKKK
                     LNLHSPDHWDTYCFAMLADYVPQDEVLSVEDEAQVDEALAWLNE"
     gene            complement(21455..22648)
                     /locus_tag="ACHKNP_RS02020"
                     /old_locus_tag="pgaptmp_000404"
     CDS             complement(21455..22648)
                     /locus_tag="ACHKNP_RS02020"
                     /old_locus_tag="pgaptmp_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4702640.1"
                     /translation="MTWDDHKKNFARLARDGGYTIAQYAAEFNLNPNTARRYLRAFKE
                     DTRTADSRKPNKPVRKPLKSMIIDHSNDQHAGDHIAAEITEKQRVNAVVSAAVENAKR
                     QNKRINDRSDDHDVITRAHRTLRDRLERDTLDDDGERFEFEAGDYLIDNVEARKAARA
                     MLRRSGADVLETTLLEKSLSHLLMLENARDTCIRLVQEMRDQQKDDDEGTPPEYRIAS
                     MLNSCSAQISSLINTIYSIRNNYRKESREAEKHALSMGQAGIVKLAYERKRENNWSVL
                     EAAEFIEAHGGKVPPLMLEQIKADLRAPKTNTDDEERQTAVGGPSLEDLDKVARERAA
                     NRRADAALWIEQRREEIADIVDTGGYGDVDTEGVPNDPWLEQDLDEDEEEDEEVTRKL
                     YGDDD"
     gene            complement(22734..23186)
                     /locus_tag="ACHKNP_RS02025"
                     /old_locus_tag="pgaptmp_000405"
     CDS             complement(22734..23186)
                     /locus_tag="ACHKNP_RS02025"
                     /old_locus_tag="pgaptmp_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Late promoter-activating protein"
                     /protein_id="HGU4702641.1"
                     /translation="MLLNWQGRHFMEINHSRITSYEIADYMIRTKSLLSAKELAAILE
                     KEYPHLDVDKRDVYLRLKAIAVSKYSSVLIDDSTRPRRFQIHSLNPEFFRRSRAPRRF
                     DEKLQNELYMTQDEKERREHQPWVMARQLFNKVARQHRHYGNATSARI"
     gene            complement(23275..24318)
                     /locus_tag="ACHKNP_RS02030"
                     /old_locus_tag="pgaptmp_000406"
     CDS             complement(23275..24318)
                     /locus_tag="ACHKNP_RS02030"
                     /old_locus_tag="pgaptmp_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4702642.1"
                     /translation="MKAVITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQPFFNSDELFRRIGGIDALVAWLRRKEGQCQAADRSWC
                     DNHIVHAERDNSAVLLCWHHDNHYRMRGFNELKETLHNNRVNWILDVARQEMGLSDGH
                     DLSIQELCWWAFMRNMMHLMPEEVCRISINKMKAATQDSGPLKEADIRPYDDRATAYV
                     QMMEERAAPMRAKVCPVDVDSDPGMAHFKIPKLQSLKLPEYMDFVASRPCCGCGAAGA
                     GAHITPYIVRHSRLCAHDIYAIPLCQSCQRDIERDRDNWEKTHGRLAMHQRLFFDYAL
                     GVGAITSHSSSVR"
     gene            complement(24346..24525)
                     /locus_tag="ACHKNP_RS02035"
                     /old_locus_tag="pgaptmp_000407"
     CDS             complement(24346..24525)
                     /locus_tag="ACHKNP_RS02035"
                     /old_locus_tag="pgaptmp_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HGU4702643.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLVGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            complement(24530..24910)
                     /locus_tag="ACHKNP_RS02040"
                     /old_locus_tag="pgaptmp_000408"
     CDS             complement(24530..24910)
                     /locus_tag="ACHKNP_RS02040"
                     /old_locus_tag="pgaptmp_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HGU4702644.1"
                     /translation="MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEISDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDTPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            complement(24910..25131)
                     /locus_tag="ACHKNP_RS02045"
                     /old_locus_tag="pgaptmp_000409"
     CDS             complement(24910..25131)
                     /locus_tag="ACHKNP_RS02045"
                     /old_locus_tag="pgaptmp_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGU4702645.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            complement(25240..25662)
                     /locus_tag="ACHKNP_RS02050"
                     /old_locus_tag="pgaptmp_000410"
     CDS             complement(25240..25662)
                     /locus_tag="ACHKNP_RS02050"
                     /old_locus_tag="pgaptmp_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702646.1"
                     /translation="MSSSAFVTVVTGVSVFVLGRLIIKGVESYISFKEQLGEISHLLL
                     SNQAKLSNPRSDLKQEIIHDLKDAAAQLRVKYELLPFYIKMLHIGFRLVPSETEIRKA
                     AQELNLIASIHDGKTGELPYEHIAKIGLLLRIPTTYSA"
     gene            complement(25797..26186)
                     /locus_tag="ACHKNP_RS02055"
                     /old_locus_tag="pgaptmp_000411"
     CDS             complement(25797..26186)
                     /locus_tag="ACHKNP_RS02055"
                     /old_locus_tag="pgaptmp_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU4702647.1"
                     /translation="MGFPSPAADYVESRISLDQQLIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCAMEGEYRIKRYRKYPRQHLEDLSTGKKEALPVDDDGYT
                     GSNAVFGVITHIINDARGGEFDDSPAI"
     gene            complement(26308..26586)
                     /locus_tag="ACHKNP_RS02060"
                     /old_locus_tag="pgaptmp_000412"
     CDS             complement(26308..26586)
                     /locus_tag="ACHKNP_RS02060"
                     /old_locus_tag="pgaptmp_000412"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702648.1"
                     /translation="MFRPRPIPPKITNGYQPNTPEIQGSEGLKPPPAPRIPPVARNVS
                     LRRELILALSPVLAARQDWVSNDDLAEKITSLVDKILQIGNEITLIHW"
     gene            complement(26859..27509)
                     /locus_tag="ACHKNP_RS02065"
                     /old_locus_tag="pgaptmp_000413"
     CDS             complement(26859..27509)
                     /locus_tag="ACHKNP_RS02065"
                     /old_locus_tag="pgaptmp_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702649.1"
                     /translation="MAQKEMTRITEERISEIISRIEMYGHGAGYTADEVLEIAQQMLA
                     LRQKEQHESNTCRLNFEQWLEQQRAKIDVDCGCVSTETFMHWLRVAYEAGNYPDIPDS
                     SVPAPGKGVTGERIRIKPHVYRELVNRLHDTAIKCAGTRQLRERISRVLGDVITPDHH
                     KQAEKSDLERCHLEAALNIKPGHTLGIIDALLVHKMARALLPLVAEKHEADHANES"
     gene            complement(27491..27865)
                     /locus_tag="ACHKNP_RS02070"
                     /old_locus_tag="pgaptmp_000414"
     CDS             complement(27491..27865)
                     /locus_tag="ACHKNP_RS02070"
                     /old_locus_tag="pgaptmp_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702650.1"
                     /translation="MMTLKHFLDRPLWAAAAGYDFNYMDCMSYTANAYDHSFSLLFNS
                     LRILPETEVGELHLWILGFIAAGVGIAVWPFIFWLVAVVVWFKCKTYRRKYFLGDGMT
                     DIAKMNIEKWTKECEKKWRKKK"
     gene            complement(27872..28165)
                     /locus_tag="ACHKNP_RS02075"
                     /old_locus_tag="pgaptmp_000415"
     CDS             complement(27872..28165)
                     /locus_tag="ACHKNP_RS02075"
                     /old_locus_tag="pgaptmp_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702651.1"
                     /translation="MWRGNNHGGSQMILTEYTFDHKTNKSRSVYLLRHNSRVRNTVLE
                     QNLTVEMDNLGNFKPTISLDDFPCGLSEREAMLKLAEWLQRLSIAIEDNWPEP"
     gene            complement(28189..28470)
                     /locus_tag="ACHKNP_RS02080"
                     /old_locus_tag="pgaptmp_000416"
     CDS             complement(28189..28470)
                     /locus_tag="ACHKNP_RS02080"
                     /old_locus_tag="pgaptmp_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGU4702652.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRLVNCKYDRL
                     IITKGYPKREDFSRRIDVPYNGYEIKTITHPHFGDKPVKVFAIKVKINS"
     gene            complement(28470..28730)
                     /locus_tag="ACHKNP_RS02085"
                     /old_locus_tag="pgaptmp_000417"
     CDS             complement(28470..28730)
                     /locus_tag="ACHKNP_RS02085"
                     /old_locus_tag="pgaptmp_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eaa protein"
                     /protein_id="HGU4702653.1"
                     /translation="MMDVKEKVLQVMRSRAALQDKALGGEYPFRMATWNLRLAMEKEF
                     PDEEWRSADLRKILMELAKDGTVSKDTHASRIGQAVWRLEVR"
     gene            complement(28727..29173)
                     /locus_tag="ACHKNP_RS02090"
                     /old_locus_tag="pgaptmp_000418"
     CDS             complement(28727..29173)
                     /locus_tag="ACHKNP_RS02090"
                     /old_locus_tag="pgaptmp_000418"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4702654.1"
                     /translation="MRQALAWLEGHSCSDDRLGKADNQPASGNQAAESNRGNEWTGNP
                     DIDNAIIMLDRIDTLENCDDDRIEAVKAVLRRLAGNSPVTPDGWISCSERMPPQDDWI
                     LIYSKHGEYMAGQVQGEYVELSDGTLSWLGNALFWMPLPEPPQEAK"
     gene            complement(29450..29584)
                     /locus_tag="ACHKNP_RS02095"
                     /old_locus_tag="pgaptmp_000419"
                     /pseudo
     CDS             complement(29450..29584)
                     /locus_tag="ACHKNP_RS02095"
                     /old_locus_tag="pgaptmp_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29581..29913)
                     /locus_tag="ACHKNP_RS02100"
                     /old_locus_tag="pgaptmp_000420"
     CDS             complement(29581..29913)
                     /locus_tag="ACHKNP_RS02100"
                     /old_locus_tag="pgaptmp_000420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702655.1"
                     /translation="MADKKKRFRKNPSLGMGDWRFFISEPGIISIEDLPPGWGLLHVV
                     NGRVRKVHGWPKGNCCWGNPEDKPFIGNKQVECDYMLSALRRMELRGHLNEIYDGVIV
                     NKKEGNAA"
     gene            complement(29918..30096)
                     /locus_tag="ACHKNP_RS02105"
                     /old_locus_tag="pgaptmp_000421"
                     /pseudo
     CDS             complement(29918..30096)
                     /locus_tag="ACHKNP_RS02105"
                     /old_locus_tag="pgaptmp_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(30098..30868)
                     /locus_tag="ACHKNP_RS02110"
                     /old_locus_tag="pgaptmp_000422"
     CDS             complement(30098..30868)
                     /locus_tag="ACHKNP_RS02110"
                     /old_locus_tag="pgaptmp_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4702656.1"
                     /translation="MSEIDYQALREAAEKATKGCYIVGHTSGNQHENITGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAAFNPNVALVLLDERERNQQYIKRRDQEN
                     EEIALTVGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRNAKLQSENAY
                     IRNRYKELDLLIGKNILVMQAAIIEWQATGDAKSGLAWIYNTLFGPGELPDESEKDAQ
                     AYFNRKYAPIDEKLMALHKWFWEQSEAERAAGIRIKGE"
     gene            complement(30865..31350)
                     /locus_tag="ACHKNP_RS02115"
                     /old_locus_tag="pgaptmp_000423"
     CDS             complement(30865..31350)
                     /locus_tag="ACHKNP_RS02115"
                     /old_locus_tag="pgaptmp_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HGU4702657.1"
                     /translation="MTGHAAILDMCCGSRMFWFDKKDDRAIFSDIRKEEHTLCDGRRL
                     IISPDLIADFRALPFADASFPVVVFDPPHLERVGDNAWMGKKYGRLNKDTWRDDLRQG
                     FKEAFRVLWTYGVLIFKWNETQIPVRQILALTDRKPVIGQRTGKGDKTHWIIFMKEGN
                     Q"
     gene            complement(31347..32048)
                     /locus_tag="ACHKNP_RS02120"
                     /old_locus_tag="pgaptmp_000424"
     CDS             complement(31347..32048)
                     /locus_tag="ACHKNP_RS02120"
                     /old_locus_tag="pgaptmp_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4702658.1"
                     /translation="MSEINYRALREIAKQATQGEWVAFISPGTGTYAVHTPGDERCED
                     VIKWTGFDGQKNAENNARHVAAFNPKVALELLGEIKCLEDTNIDATCRISELETNLAA
                     LVAENAGLKHAMAVTLEHVSVTDAGQAGVAAMIINDALHHSETPATDAFLAEIRAEAR
                     NEGINYTASRLAAAFNHGFINKSLREVFDVTRMILSAKEELANEPYPIDGLSGEYAEK
                     SLEEWAEQIRKGGNQ"
     gene            complement(32045..32284)
                     /locus_tag="ACHKNP_RS02125"
                     /old_locus_tag="pgaptmp_000425"
     CDS             complement(32045..32284)
                     /locus_tag="ACHKNP_RS02125"
                     /old_locus_tag="pgaptmp_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702659.1"
                     /translation="MFKQNEKAIAQIADYIPRACRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNSHTRESFFSCIEAQLRKGGSQ"
     gene            complement(32277..32480)
                     /locus_tag="ACHKNP_RS02130"
                     /old_locus_tag="pgaptmp_000426"
     CDS             complement(32277..32480)
                     /locus_tag="ACHKNP_RS02130"
                     /old_locus_tag="pgaptmp_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702660.1"
                     /translation="MTIVKTHTGTVITKDGPQVKKLHQTERMWVVGKNEFYHKDTGRR
                     HFAENTRRRLLLDTIKPIEVKHV"
     gene            complement(32564..33280)
                     /locus_tag="ACHKNP_RS02135"
                     /old_locus_tag="pgaptmp_000427"
                     /pseudo
     CDS             complement(32564..33280)
                     /locus_tag="ACHKNP_RS02135"
                     /old_locus_tag="pgaptmp_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675631.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33475..33981)
                     /locus_tag="ACHKNP_RS02140"
                     /old_locus_tag="pgaptmp_000428"
     CDS             complement(33475..33981)
                     /locus_tag="ACHKNP_RS02140"
                     /old_locus_tag="pgaptmp_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-phosphatase"
                     /protein_id="HGU4702661.1"
                     /translation="MSIFNKHAHQERPYIVIVDIDGTISEATEDRLHLLPPPGKGALT
                     KDWNEFNLACDTDTPITPVIDMVRQLFNVYTVWFVTGRCEIARDKTRAWLRKYVTNGA
                     EPLLSMRPATDDRNDGPAKIDLLKKIGLSKIAFALEDKIEVARVFRSHGVLTFMVREY
                     ENALLHQQ"
     gene            complement(34054..35316)
                     /locus_tag="ACHKNP_RS02145"
                     /old_locus_tag="pgaptmp_000429"
     CDS             complement(34054..35316)
                     /locus_tag="ACHKNP_RS02145"
                     /old_locus_tag="pgaptmp_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702662.1"
                     /translation="MSTSAQNQSIENVSIPDVLNAGIPAIIQNIRAAQRRVSCDDLTA
                     RFFDNAVQSAEMLHAQLIDVYNAEADSHNSLVDAAENMQLDLGLKGKEIEELQLQIEH
                     LKRQQQDAIDDATHDANQRADNAERISIELETKLNEMTAMVELRNSQISTLKSQYKEI
                     MKLDPFNLEKRYNKAKSERQELRKQVADLNQQLKKTIKDASEARVAFANKKAEVTALV
                     NENAKFATLKKEMYGITERRFPASKLHPTLGQISFFPRLLAYGISSPKEFNNERPYIV
                     SKLDFAYQFCCDMGYAIDIRINEWLMPNFQPLAIFREFQPEGWVEFFHELICKEMESR
                     RPELVRRVEWAQEVMLADAELPFEPEFIDDLATKGLHTLFDVVTRRHEQLVVELGLEE
                     TAARRLLDVCYARSDAWEKENGGTIYVR"
     gene            complement(35618..36319)
                     /locus_tag="ACHKNP_RS02150"
                     /old_locus_tag="pgaptmp_000430"
     CDS             complement(35618..36319)
                     /locus_tag="ACHKNP_RS02150"
                     /old_locus_tag="pgaptmp_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702663.1"
                     /translation="MKIALVFRSGGDYNASDVQWLVNQLPKGYEIICLTDLKRLHVPG
                     VKVVPLINQWQKCRGWWAKIELFRPDITDDLFYLDLDTVIAGDIRPILEHPPTSFTML
                     RDFYHPQYRGSGALWIPNSVKAHIWSSFWQDPEGWISRCVTTECWGDQGFLQKVMGDD
                     TPAFQDLYPGWFVSYKADVVEPGSKYASARYSRGNGALPKDCRIIFFHGKPRPREVSE
                     DWLPLISSFFERESE"
     gene            complement(36316..36993)
                     /locus_tag="ACHKNP_RS02155"
                     /old_locus_tag="pgaptmp_000431"
     CDS             complement(36316..36993)
                     /locus_tag="ACHKNP_RS02155"
                     /old_locus_tag="pgaptmp_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGU4702664.1"
                     /translation="MMAPTIYHRIDGTKYRNVWVVGDLHGCYTRLMSELHRVDFDPAQ
                     DLLISVGDLIDRGTENVECLELLQMPWFRAVMGNHERLMIDALSPDGNVNNWLMNGGQ
                     WFFMLDTDQEILAWALVELVKRLPYIIELNTGQETIVIAHADYPDNEYQFGKEVPLFN
                     VVWARERISDSMDDIGGEISGADRFIFGHTPVKSPKTFWNQQYIDTGAVFCGNLTLMK
                     VKGDGAA"
     gene            complement(36990..37616)
                     /locus_tag="ACHKNP_RS02160"
                     /old_locus_tag="pgaptmp_000432"
     CDS             complement(36990..37616)
                     /locus_tag="ACHKNP_RS02160"
                     /old_locus_tag="pgaptmp_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702665.1"
                     /translation="MFPKNKFRGSDRVIIVGSGPSAANFVAPRGVPIIAVNGAIDWLN
                     RASYFFTLDPSPDNMRRVGRGRRRRGVCYCMALPDVKEREVRDGVLCFRRVAERGMEP
                     KNTNSPEWWAWRWSAHFGLCEDENEIASGNSAYGALNLAFHIGFKHVALVGVDATQEP
                     RVHSGGTPKNLSHLPLLFQSARERIDVVSCGKMGGIPQMTLKEWLKNT"
     gene            complement(37514..38014)
                     /locus_tag="ACHKNP_RS02165"
                     /old_locus_tag="pgaptmp_000433"
     CDS             complement(37514..38014)
                     /locus_tag="ACHKNP_RS02165"
                     /old_locus_tag="pgaptmp_000433"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702666.1"
                     /translation="MFDDIYALYAGDLSWWKQYGSTIPGGKFRKVTANLAAAKSFSLE
                     YRRYCGPAEGVNSGAQAISLAAESGAEVVVLVGYDCSLQNGLHWHGAHPQALRNPTQV
                     SISKWQQQFLDTRKKHADLHILNASRSSAIQCFPRINLEAVIALLSSAVAQAPQTLLR
                     RAECRL"
     gene            complement(38118..38273)
                     /locus_tag="ACHKNP_RS02170"
                     /old_locus_tag="pgaptmp_000434"
     CDS             complement(38118..38273)
                     /locus_tag="ACHKNP_RS02170"
                     /old_locus_tag="pgaptmp_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702667.1"
                     /translation="MSSKANYESLASIMPRNEQEADAVVDPVIAEMNARLEAEFAAEN
                     EHTTQGD"
     gene            complement(38341..38919)
                     /locus_tag="ACHKNP_RS02175"
                     /old_locus_tag="pgaptmp_000435"
     CDS             complement(38341..38919)
                     /locus_tag="ACHKNP_RS02175"
                     /old_locus_tag="pgaptmp_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HGU4702668.1"
                     /translation="MLRFTEEEFQAFSERRNKGQSRPKTKKDPFLSLAPVKEVSPHAK
                     ALAALAKNPDLRDGNCEHFEQVFIFDYFERKHPDIYELLHATPNGGKRSKATAGKMKA
                     EGQKKGYPDMSLDKACGIYHGMRIELKEPNGKAPTKEQIAWMRRLREEGYNVVLAYGA
                     EQAITAILEYMSLKKGEAIEHVLNGDKWLYAT"
     gene            complement(38922..39167)
                     /locus_tag="ACHKNP_RS02180"
                     /old_locus_tag="pgaptmp_000436"
     CDS             complement(38922..39167)
                     /locus_tag="ACHKNP_RS02180"
                     /old_locus_tag="pgaptmp_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702669.1"
                     /translation="MAERVDDAELSMNQLEALKDMAIDNIRKQAQVVSQVFTGKCRYC
                     NEPIESGIYCDAECAQWHREEQAAKQRKYGMRPAGFD"
     gene            39314..39691
                     /locus_tag="ACHKNP_RS02185"
                     /old_locus_tag="pgaptmp_000437"
     CDS             39314..39691
                     /locus_tag="ACHKNP_RS02185"
                     /old_locus_tag="pgaptmp_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702670.1"
                     /translation="MATSITTTQSTRQYPLSRYDDRNIADPILRAELRKEVMLMCESN
                     DKNLTIYYVLPDEQYRPDLLAYRMWGIAELRWVVTLAAGLEDESQGMTVGKKLKLPPA
                     TWIREMIRHFQYDGQVIGTLSIA"
     gene            39701..40918
                     /locus_tag="ACHKNP_RS02190"
                     /old_locus_tag="pgaptmp_000438"
     CDS             39701..40918
                     /locus_tag="ACHKNP_RS02190"
                     /old_locus_tag="pgaptmp_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4702671.1"
                     /translation="MPTEYARDNLGRYQTDGLSAKDFNKVFDLIRKQQRQNRRNARRT
                     LTPRIMGMRNRELEAFLSLGKKKDGTYFTPEDIRSFNTSRQAHKTKFKSTVPGITYAQ
                     LVAQSTSIDIKRANNKVSDGTGIKAATFLGLKHNLALISVNASDESVHQHHRVRIRFE
                     EWDKAVEDIAEDGAKKARIAADLCKGRVSFDCDCGRHQYWYRYMATAGNYAVAPPKEY
                     AFPKIRNPDLTGVACKHVLHAMTRFQSPTWHKAIIIALEKAAEQVAFGDDKRKTTTYF
                     KGELAKSLARNRTTTTDQAKAAREYELYLKSQDALGKKLRAKDSATDNVRRLLKKART
                     TANRKNAELKASRVREAQARAEADALKKALQTQANNLIKFFMSQGMDKAAATAQARSI
                     LETQINEARKRKG"
     gene            40922..41650
                     /locus_tag="ACHKNP_RS02195"
                     /old_locus_tag="pgaptmp_000439"
     CDS             40922..41650
                     /locus_tag="ACHKNP_RS02195"
                     /old_locus_tag="pgaptmp_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4702672.1"
                     /translation="MAGFFDDMFEDTEPSQQVTGDNLPDTESDPDIPGEGSELIEEED
                     IDAEIETDGVNVGNIVDPVEDNHLPNLDHGLLSDSGVRHRYQGHAVFNNLVRMDWLKA
                     IKLDPDSFDAVLYRAIPYINKNAPETAPEIIEPNQRIYDYQDPELITALDCPDEMDAF
                     YALYDGSDNTGISDSALILRLAAVNVPVGSMLEWLEQLSDGTTIRRFWYIHKIFNYGT
                     ARVGSLFYCVPSRAFEGNFIGDSE"
     gene            41637..42422
                     /locus_tag="ACHKNP_RS02200"
                     /old_locus_tag="pgaptmp_000440"
     CDS             41637..42422
                     /locus_tag="ACHKNP_RS02200"
                     /old_locus_tag="pgaptmp_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate"
                     /protein_id="HGU4702673.1"
                     /translation="MILNNQEWLLAIFKKKGLTPTGKLEFATIDGIDSALAQALNEAF
                     DSQVVSFNDRTNQSFREFLKRTPRDRITLGTFSDVKEWLSSFEADRAGRKDTASAGPV
                     NKLAMPLVNLSRSPAFSIYEGELCRDNYDEGHVTNENDEIEALVSTIPFSLEYSLWIA
                     SDEKESLGMVTTALAFWLRMYASLGQASFTHIANVGGYEIPVTCYIEGQKSIAFQDLT
                     TGTADNRLFAVGLNLTVVAELPILAYMQQTTGTITVKAKILEE"
     gene            42424..43440
                     /locus_tag="ACHKNP_RS02205"
                     /old_locus_tag="pgaptmp_000441"
     CDS             42424..43440
                     /locus_tag="ACHKNP_RS02205"
                     /old_locus_tag="pgaptmp_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4702674.1"
                     /translation="MATKTTTAPETDSKRTQLFLQSVSIGQNEIPREMIVGCTYVEPG
                     ELSGPQLMLMVRDSTAYVVNKLGVKFGTILTVSLGDPEGHGGILFSEEFFVLKAPRKD
                     DTVLIYAFSNPVRLLKVPSTSAQYFVDKPPSAVVSSLAPGLKVNADSFRKTSTYHLNV
                     GEKPTKVLQEIARDTGSMCWASRGTINFKSMEKMANAAPSLTYESANPNTSGFTISQF
                     NILNADYEYQRRHNYRMASYDMTKGVVYSGNQEDPIKFTSNPDPTALANYNKFILPRL
                     DMLVEGNAALTPGTTLKIVVHNTAGDGELDESIPDKMIVMSVTHFEDRFRFVSRAQLG
                     VVNG"
     gene            43433..44065
                     /locus_tag="ACHKNP_RS02210"
                     /old_locus_tag="pgaptmp_000442"
     CDS             43433..44065
                     /locus_tag="ACHKNP_RS02210"
                     /old_locus_tag="pgaptmp_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HGU4702675.1"
                     /translation="MGSLTGKYRAVVVSVDDPKGLMRTQIRVVGMMDGLPDASLPWAE
                     AILSNANTFSPFLPGDKVWVEFPYNGDSRWPLIIGYAQDASGGAPNVPPEASGQGEGY
                     VPPEVEGAPAQPSTSAKKDFISSRNGLMEVRTAGGAWAVTHLKSGTTIGFNEAGELYA
                     ISQGPAFISSAGNLDIKSGADVALKAGGSMAIEASGNLSIKAAQVSVDKA"
     gene            complement(44112..45110)
                     /locus_tag="ACHKNP_RS02215"
                     /old_locus_tag="pgaptmp_000443"
     CDS             complement(44112..45110)
                     /locus_tag="ACHKNP_RS02215"
                     /old_locus_tag="pgaptmp_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702676.1"
                     /translation="MQWNEEKPMNILIIGRKFEAISDVKTYTEMWAYNLACAFSEAGV
                     TLQFHRPYSPGVESPEDYVEAVLTAATSCSAKAILAPGLRYFTTVPREIGVQLRRRFT
                     GWVAQVYDGSMLDSAPVDITFTVRDDTWRYLDNPGRLERHNRFNKHVGWAANQDLFHL
                     ETKTDDVLRIFVDHAAFDVSGFDHSLSILMNLQRLTVPYEARTLTDDGLITIDPGNIS
                     VTPYRRTPVPATEFAAELRKSDVFIVTHPESLGLTVLEAAMCGALVLTPPDCLPPDRL
                     ALVNHMVIKSRIDWDEVIARVDRVKNAEKVQCHTWSAIAEKMLETFITQKPSRGNG"
     gene            complement(45110..46474)
                     /gene="dnaB"
                     /locus_tag="ACHKNP_RS02220"
                     /old_locus_tag="pgaptmp_000444"
     CDS             complement(45110..46474)
                     /gene="dnaB"
                     /locus_tag="ACHKNP_RS02220"
                     /old_locus_tag="pgaptmp_000444"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU4702677.1"
                     /translation="MSASPLESMPNSLSAEQAVLGGLMLDNCRWDEVADRIVADDFYT
                     SAHREIFSEMERLLSHGKPIDLITLAEALEQNGKLERAGGFAYLAEMSKNTPSAANIC
                     AYADIVRERAVVREMISVANEIAEAGYAQDGRGSNELLDMAERRVFEIAEKRQKSGSG
                     PKDIASILDATVSRIEELFQRPHDGVTGLDTGFTDLNKKTAGLQASDLIIVAARPSMG
                     KTTFAMNLVENAAVRNDKPVLVFSLEMPSHQLMMRSLASLARVDQTRIRTGQLNDEDW
                     ARVSGAMGILLDKQNIFIDDSSALTPTELRSRARRVYKENGGLSMIMIDYLQLMRVPE
                     LQDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGAIEQ
                     DADLIMFLYRDEVYHPDSEMKGIAEVIIGKQRNGPIGTVRLAFNGQYSRFDNYAGADW
                     QEDY"
     gene            complement(46755..46830)
                     /locus_tag="ACHKNP_RS02225"
                     /old_locus_tag="pgaptmp_000445"
     tRNA            complement(46755..46830)
                     /locus_tag="ACHKNP_RS02225"
                     /old_locus_tag="pgaptmp_000445"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46795..46797),aa:Ile,seq:cat)
     gene            complement(47892..47967)
                     /locus_tag="ACHKNP_RS02230"
                     /old_locus_tag="pgaptmp_000446"
     tRNA            complement(47892..47967)
                     /locus_tag="ACHKNP_RS02230"
                     /old_locus_tag="pgaptmp_000446"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47932..47934),aa:Thr,seq:tgt)
     gene            complement(47972..48058)
                     /locus_tag="ACHKNP_RS02235"
                     /old_locus_tag="pgaptmp_000447"
     tRNA            complement(47972..48058)
                     /locus_tag="ACHKNP_RS02235"
                     /old_locus_tag="pgaptmp_000447"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48022..48024),aa:Tyr,seq:gta)
     gene            complement(48064..48138)
                     /locus_tag="ACHKNP_RS02240"
                     /old_locus_tag="pgaptmp_000448"
     tRNA            complement(48064..48138)
                     /locus_tag="ACHKNP_RS02240"
                     /old_locus_tag="pgaptmp_000448"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48104..48106),aa:Asn,seq:gtt)
     gene            complement(48811..51852)
                     /locus_tag="ACHKNP_RS02245"
                     /old_locus_tag="pgaptmp_000449"
     CDS             complement(48811..51852)
                     /locus_tag="ACHKNP_RS02245"
                     /old_locus_tag="pgaptmp_000449"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HGU4702678.1"
                     /translation="MKELCYGSVCSGIEAASIAWEPLGMRPAWFAEIEPFPSAVLALR
                     WPHVANLGDMKKLAKKVLAGEIESPDVLVGGTPCFTAGHMVLCKNGYKPIENVCPGDY
                     VVSHLGRLQQVKRVGSKIANTGLLNAVGQPLGIRTTNDHPFLAVRWKAQNTRKNGTYF
                     KRELLSEPEWRAACDMPGYQWCALTNFNIAFPDICSRFLSEEQAMYLAGAYVGDGYIR
                     RWRGKSKKAVVFGINCQKLRKFHCRIPENIFSVASEIRGSIKVTLNDTCYANWLNEHF
                     GELSHAKRIPAWVMSHPLRHVFLQGYLDTDGTPSGKAGFRINSVSSALAWGVAGLSQT
                     CGYVSSVSFIEVEPKKVIEDRVVNQRNYYQVTICPQKLSRKSRLAHGMLLRTVKEFKS
                     VGLDTVYNIEVEGDHSYILNGAVVHNCQAFSIAGLRGGLDDERGALTLKYVELANAID
                     DKRAESFLKPAVIVWENVPGVLSSADNAFGCFLAGLAGEDVPFEPGDRPESGKSNAFW
                     RWDGKTGCHVPKWPQCGCIYGPQRKVAWRILDAQYFGVAQRRRRVFVVASARTDLDPA
                     TVLFEFEGVRRNIAPSRGEGKETTRYTSNIAIRTCDDTNIIAMAHGQGGAEIKTDNSA
                     PTLTCNHEAPIVLLGDGRMRRLTPVECERLQGFPDWHTLIPTEKRKKVNSDELAYLHN
                     HYPDLSEEEAAMLAADGPRYKAIGNSMAIPVMRWIGDRITKAVCRQKEGSETKERKVK
                     PAAEFERSIFKWAGGKFGVLEQIFRYLPEGKRLIEPFVGGGAVFMNAGYQENLLNDVN
                     ADLINFYKTLQREAHSLITLAHRFFQDYNTQEGYLAVRNAFNKQVYDDLHRAAAFLFL
                     NRHCFNGLTRYNQAGEFNVGYGKYKTPYFPLQEMEAFLGAEGRSEFVCGDFAAVIEAA
                     GEGDVIFCDPPYEPLPNTEGFTNYSGHDFKFEEQKRLVSLLTDAHRRGAKVLITNSGA
                     PNIRELYHDSGFRVEPLFARRSVSCKGDTRGVAHDVLGILL"
     gene            complement(51849..52754)
                     /gene="rdgC"
                     /locus_tag="ACHKNP_RS02250"
                     /old_locus_tag="pgaptmp_000450"
     CDS             complement(51849..52754)
                     /gene="rdgC"
                     /locus_tag="ACHKNP_RS02250"
                     /old_locus_tag="pgaptmp_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HGU4702679.1"
                     /translation="MFKHWKNITIYKLSREADLTDLEDKKKMILFTPCGSQDMAKFGF
                     VSPFGDNSEVIAMHGNGFILVEAKRETKILPPPVIQRAIQEKIEKLEQEQARKLKKTE
                     KDSLKDEVLHSLLPRAFSKFSVIQAIYDGSTKRIYINASARQAEDMLALMRKSLGSLP
                     AVPLSVENPIELTLTDWVRDGSAPQGFQMGDAAELKAVLEDGGIARVKKQDLGSDEIS
                     THLEAGKLVTKLALDWQNRIKFTLDHNFSLTSVKFADELLEQNSDIDSEDVAQRLDAD
                     FFLLTSEISCLVDALVNALGGEAKQ"
     gene            complement(52747..53031)
                     /locus_tag="ACHKNP_RS02255"
                     /old_locus_tag="pgaptmp_000451"
     CDS             complement(52747..53031)
                     /locus_tag="ACHKNP_RS02255"
                     /old_locus_tag="pgaptmp_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702680.1"
                     /translation="MQMELISRKEFDSRVTSGELDNLQAIKVKEGFCLIGNQSGINRV
                     FMLRRTDLKPFVWKNEIGPSSYAQTRGCHNLAFFYKDELSVVDIQGLQHV"
     gene            53494..54282
                     /locus_tag="ACHKNP_RS02260"
                     /old_locus_tag="pgaptmp_000452"
     CDS             53494..54282
                     /locus_tag="ACHKNP_RS02260"
                     /old_locus_tag="pgaptmp_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702681.1"
                     /translation="MLEKDYQLSAYKKLAAAGGMKTPGAITSARNSANTAKLLAEELT
                     GLILDTIVYPDTITSYVSTIRTTTTGLTNIGELATKHADLLAGYADLSMLLQLDIGWD
                     VYCRANEREVSELPISIAIGDVNITKSLEDAVNALNTSSLVAAMGEINQALNTGSESS
                     SGSGSGGGTATPPPALTEEQIESLKVATEQFGVVFNQTTAPTTALQQQYERANESANV
                     AITAYNHAIGTALAEASANKASTASAVAALVPDSVLDELNKAAR"
     gene            54322..54744
                     /locus_tag="ACHKNP_RS02265"
                     /old_locus_tag="pgaptmp_000453"
     CDS             54322..54744
                     /locus_tag="ACHKNP_RS02265"
                     /old_locus_tag="pgaptmp_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ppfA"
                     /protein_id="HGU4702682.1"
                     /translation="MSFFSTLKTALSLKEKLAATGVLVLICALVGAGFAWERHQLKQA
                     MEKIGSLDQAVKERDKSIMDLNQTIETMNKAEQHFHSQEVKNESEQAKYADRQMERKA
                     EVQKQLVAAGNVRQRIPADTQRLLRESISEFNADADKG"
     gene            54770..54910
                     /locus_tag="ACHKNP_RS02270"
                     /old_locus_tag="pgaptmp_000454"
     CDS             54770..54910
                     /locus_tag="ACHKNP_RS02270"
                     /old_locus_tag="pgaptmp_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702683.1"
                     /translation="MCRMPEFSSEYFDDLPAYILDTETMLMGINRKNRNVNDYNRAIS
                     GN"
     gene            54922..55314
                     /locus_tag="ACHKNP_RS02275"
                     /old_locus_tag="pgaptmp_000455"
                     /pseudo
     CDS             54922..55314
                     /locus_tag="ACHKNP_RS02275"
                     /old_locus_tag="pgaptmp_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281114.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            55648..56532
                     /locus_tag="ACHKNP_RS02280"
                     /old_locus_tag="pgaptmp_000456"
     CDS             55648..56532
                     /locus_tag="ACHKNP_RS02280"
                     /old_locus_tag="pgaptmp_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HGU4702684.1"
                     /translation="MLEENKGFLSVEEVAGNTGEIHSLKPNNNSTIQPIALLRLGVFV
                     PTLKSTNVALRRGSSVTTNTTNATEELSSLKIVEQEGYEGIEIHGPRLDMDTDFKVWV
                     GITSALFDYAPDDDGIITLPFSEFADRCGYPRKRLSKAFRKSIDDSLTRIQQTVVKFR
                     FPAAKGHLNNINVNLLAYSSLNTELDVIEIQPQKQLSELYYVDYKRILKLKMLDKLGR
                     KETAKVLYTFFEALPANPAPVSIERLRARLNLKSSVSVQNSVIRKAMKDLEAIEYLKF
                     SEIKNGRKIGFQIHKRNP"
     gene            56825..57160
                     /locus_tag="ACHKNP_RS02285"
                     /old_locus_tag="pgaptmp_000457"
     CDS             56825..57160
                     /locus_tag="ACHKNP_RS02285"
                     /old_locus_tag="pgaptmp_000457"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HGU4702685.1"
                     /translation="MQEEKQHYLYVLVPENGDTFKIGISCGPLARFKGLQVSPDFALS
                     RVYRGTRLAMVNLERALHATFFPWNAPWEKSAGGGHTEWFTRECLDKVLAHIEYLNDM
                     WGGILERIK"
     gene            57263..57634
                     /locus_tag="ACHKNP_RS02290"
                     /old_locus_tag="pgaptmp_000458"
     CDS             57263..57634
                     /locus_tag="ACHKNP_RS02290"
                     /old_locus_tag="pgaptmp_000458"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702686.1"
                     /translation="MAYVSISGYEPDAIRAQCELLKAMFMLRTKYPWETGRVCFPMEE
                     LTATIDSQLYHDNPEKFFSLLAGNGLNCVSGLGRSRIQHASLFGPFFYDRHGYFEAEL
                     PALTRAIDTIDFERLFAALSK"
     gene            57803..58999
                     /locus_tag="ACHKNP_RS02295"
                     /old_locus_tag="pgaptmp_000459"
     CDS             57803..58999
                     /locus_tag="ACHKNP_RS02295"
                     /old_locus_tag="pgaptmp_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4702687.1"
                     /translation="MSDSSQLHKVAQRANRMLNVLTEQVQLQKDELHANEFYQVYAKA
                     ALAKLPLLTRANVDYAVSEMEEKGYVFDKRPAGSSMKYAMSIQNIIDIYEHRGVPKYR
                     DRYSEAYVIFISNLKGGVSKTVSTVSLAHAMRAHPHLLMEDLRILVIDLDPQSSATMF
                     LSHKHSIGIVNATSAQAMLQNVSREELLEEFIVPSVVPGVDVMPASIDDAFIASDWRE
                     LCNEHLPGQNIHAVLKENVIDKLKSDYDFILVDSGPHLDAFLKNALASANILFTPLPP
                     ATVDFHSSLKYVARLPELVKLISDEGCECQLATNIGFMSKLSNKADHKYCHSLAKEVF
                     GGDMLDVVLPRLDGFERCGESFDTVISANPATYVGSADALKNARIAAEDFAKAVFDRI
                     EFIRSN"
     gene            59016..60017
                     /locus_tag="ACHKNP_RS02300"
                     /old_locus_tag="pgaptmp_000460"
     CDS             59016..60017
                     /locus_tag="ACHKNP_RS02300"
                     /old_locus_tag="pgaptmp_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HGU4702688.1"
                     /translation="MSKKNRPTIGRTLNPSILSGFDSSSASGDRVEQVFKLSTGRQAT
                     FIEEVIPPNQVESDTFVDQHNNGRDQASLTPKSLKSIRSTIKHQQFYPAIGVRRGTGK
                     IEILDGSRRRASAILENVGLRVLVTDQEISVQEAQNLAKDVQTALQHSIREIGLRLMR
                     MKNDGMSQKDIAAKEGLSQAKVTRALQAASAPEELVALFPVQSELTFSDYKTLCAVGD
                     EMGNKNLEFDQLIQNISPEINDILSIEEMAEDEVKNKILRLITKEASLLTDKGSKDKS
                     VVTELWKFEDKDRFARKRVKGRAFSYEFNRLSKELQEELDRMIGHILRKSLDKKPKS"
     gene            60242..61948
                     /locus_tag="ACHKNP_RS02305"
                     /old_locus_tag="pgaptmp_000461"
     CDS             60242..61948
                     /locus_tag="ACHKNP_RS02305"
                     /old_locus_tag="pgaptmp_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702689.1"
                     /translation="MSNLREYQNRIADIAKRSKAVLGWASTAQFGTDNQFIKDDAARA
                     ASILEAARKDPVFAGISDNATAQIATAWASALADYAAAHKSMPRPEILASCHQTLENC
                     LIESTRNSMDATNKAMLESVAAEMMSVSDGVMRLPLFLAMILPVQLGAATADACTFIP
                     VTRDQSDIYEVFNVAGSSFGSYAAGDVLDMQSVGVYSQLRRRYVLVASSDGTSKTATF
                     KMEDFEGQNVPIRKGRTNIYVNRIKSVVDNGSGSLLHSFTNAAGEQITVTCSLNYNIG
                     QIALSFSKAPDKGTEIAIETEINIEAAPELIPLINHEMKKYTLFPSQFVIAAEHTVQA
                     AYEAQREFGLDLGSLQFRTLKEYLSHEQDMLRLRIMIWRTLATDTFDIALPVNQSFNV
                     WATIIRGKFQTVYRDIIERVKSSGAMGMFAGADAASFFKQLPKDFFQPAEDYIQTPYV
                     HYIGTLFGNVKVYEVPAGICKNLTTENIQFSSMDVLCYVRDENPGKAGFVTGDAVPAI
                     PFQHPTTPALVNRTTLWGSAINDMHPRNGADYFTRVTLTMAKKGGLNFISGDTIDAGD
                     SE"
     gene            62009..63598
                     /locus_tag="ACHKNP_RS02310"
                     /old_locus_tag="pgaptmp_000462"
     CDS             62009..63598
                     /locus_tag="ACHKNP_RS02310"
                     /old_locus_tag="pgaptmp_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702690.1"
                     /translation="MSQYSIQQSLGNASGVAVSPINADATLSTGVALNSSLWAGIGVF
                     ARGKPFTVLAVTESNYKDVLGEPLKPSSGSQFEPIRHVYEAIQQTSGYVVRAVPDDAK
                     FPIIMFDESGEPAYSALPYGSEIELDSGEAFAIYVDDGDPCISPTRELTIETATADSA
                     GNERFLLKLTQTTSLGVVTTLETHTVSLAEEAKDDMGRLCYLPTALEARSKYLRAVVN
                     EELISTAKVTNKKSLAFTGGTNGDQSKISTAAYLRAVKVLNNAPYMYTAVLGLGCYDN
                     AAITALGNICSDRLIDGFFDVKPTLTYTEALSAVEDTGLLGTDYVSCAVYHYPFSCKD
                     KWTQSRVVFGLSGVAYAAKARGVKKNSDVGGWHYSPAGEERAVIARASIQPLYPEDTP
                     DEEAMVKGRLNKVSVGTSGQMIIDDALTCCTQDNYLHFQHVPSLMNAISRFFVQLARQ
                     MKHSPDGITAAGLTKGMTKLLDRFVASGALVAPRDPDADGTEPYVLKVTQAEFDKWEV
                     VWACCPTGVARRIQGVPLLIK"
     gene            63608..64423
                     /locus_tag="ACHKNP_RS02315"
                     /old_locus_tag="pgaptmp_000463"
     CDS             63608..64423
                     /locus_tag="ACHKNP_RS02315"
                     /old_locus_tag="pgaptmp_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702691.1"
                     /translation="MSKNFFQSGAFLGNGLSRFALNSDPVQLMESARASAEQPTDPVI
                     NNNPEPAAQTNDNVPSAPAPEQILEGKDGKEWTVEQAHQMILEAANRSAMQNALSDAA
                     DAVFAWADSGDLTFDSLDGFVQAIAGISDDDDSEVTEEQDDAYNEAWANVADFLAACG
                     VDDDLIEALADDEDDDAAADVGASIAGLDSDDRDELEAAFVVAGTSDEMLTEAFKKVV
                     RNGEIKLIRKRLRKKRLTAAQKSALKKARRKAQTGAAKLARKKSMKLRRKRLG"
     gene            64582..65040
                     /locus_tag="ACHKNP_RS02320"
                     /old_locus_tag="pgaptmp_000464"
     CDS             64582..65040
                     /locus_tag="ACHKNP_RS02320"
                     /old_locus_tag="pgaptmp_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702692.1"
                     /translation="MAQNWEAPFTGMSMGSVAGAFSGFAQVGTETTSVARWNSLMVWE
                     GGTPPTFTLPVTFIALFDPFTEVSGAIAALSAMISPELKDASIGGRIPERVTLNIGRQ
                     INIIDVAIQDISFDLDAPRDSNGHFLKNTVNLQLTGSSIYNSSDIVRAFQ"
     gene            65052..65561
                     /locus_tag="ACHKNP_RS02325"
                     /old_locus_tag="pgaptmp_000465"
     CDS             65052..65561
                     /locus_tag="ACHKNP_RS02325"
                     /old_locus_tag="pgaptmp_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HGU4702693.1"
                     /translation="MGHNNTKGNRKFIKGRYTANAAKGERLVSSEFQLTVAGHEDISV
                     LVRTSQIPEMTREDVEDYGPNGVKFNQHGPIRNSGEIQVQCVETIEGDILQFIKDRIA
                     AKDYVDITMAATPESKSSGVNAVTKAATTIEMLDCKIYSDAIDFSTEDVTAAVRPSLR
                     IVYNWIEWD"
     gene            complement(65678..65833)
                     /gene="hokD"
                     /locus_tag="ACHKNP_RS02330"
                     /old_locus_tag="pgaptmp_000466"
     CDS             complement(65678..65833)
                     /gene="hokD"
                     /locus_tag="ACHKNP_RS02330"
                     /old_locus_tag="pgaptmp_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU4702694.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVTVF
                     TAYESER"
     gene            complement(66015..66260)
                     /locus_tag="ACHKNP_RS02335"
                     /old_locus_tag="pgaptmp_000467"
     CDS             complement(66015..66260)
                     /locus_tag="ACHKNP_RS02335"
                     /old_locus_tag="pgaptmp_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702695.1"
                     /translation="MQSDFLQLAIAFAGYVCIGFCVYMISRKMLVDIDRKEQAEEILV
                     WIFFGAVWPLGIMFAATFLLMWIFTLPGDFYRKKARH"
     gene            complement(66311..67156)
                     /locus_tag="ACHKNP_RS02340"
                     /old_locus_tag="pgaptmp_000468"
     CDS             complement(66311..67156)
                     /locus_tag="ACHKNP_RS02340"
                     /old_locus_tag="pgaptmp_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGU4702696.1"
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCA
                     NLAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANL
                     YRFTPSFLAFAQQAKNALIESKLKISSAATKVKAVLAKTLALFNFLSTPPCQNDTPSP
                     CQDDVAIKNKKSQFKKTKRSVSGAAGTTKLKKLTSWIAEAKAKADNLRLSKKRAQKHE
                     FKQKVEAAARKYAYLKNKRSPDIGGVSNFDNLPHCMTVNEALNAVLAKNKDNEQWGIP
                     AGFRG"
     gene            complement(67186..67986)
                     /locus_tag="ACHKNP_RS02345"
                     /old_locus_tag="pgaptmp_000469"
     CDS             complement(67186..67986)
                     /locus_tag="ACHKNP_RS02345"
                     /old_locus_tag="pgaptmp_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU4702697.1"
                     /translation="MQQTFNADMNISNLHQNVDPSTTLPVICGVEITTDRAGRYNLNA
                     LHRASGLGAHKAPAQWLRTLSAKQLIEELEKETMQNCIVSFEGRGGGTFAHELLAVEY
                     AGWISPAFRLKVNQTFIDYRAGRLQPAIPQSLPEALRLAADLAEQKQRLEQKMLMDAP
                     KVEFAERVATASGVLIGNYAKVLGLGQNYLFTWLRDNGILIATGERRNVPKQEYISRG
                     YFTLKETVIDTSNGSRISFTTRITGKGQQWLMKRLLDAGVLVPVAAAR"
     gene            complement(68150..69241)
                     /locus_tag="ACHKNP_RS02350"
                     /old_locus_tag="pgaptmp_000470"
     CDS             complement(68150..69241)
                     /locus_tag="ACHKNP_RS02350"
                     /old_locus_tag="pgaptmp_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU4702698.1"
                     /translation="MRTSLVTREEMIEAIEQHTACISTRDIPGVIANYFMITKQLYRR
                     KDKHAVHRILLTDIREYLLEQGHLNYATVAAEARKEAHRMKATNVKSEKIYAPSVRES
                     ELVVVQNQSDEIPVLEWLGVRVVTTETLARGYGTDEANIRKNLSRNSDRFEEGKHYYL
                     LTGSKLKEFKRLVTISHLVSKYTSQVILWTERGAARMSKIVDTNEAWAFFEKLEDSYF
                     RQKEQQPVSIPQTLPEALRLAAELAEQKMQLEQQLVAAAPKVDFADRVSVAKGILIGN
                     FAKVVGLKQNALFAWLRENGILIASGGRKNVPFQQYINAGYFTVKEVVLDDEDGYQIR
                     LTPQLTGKGQQWLTRKLLDAGLLKPVAIG"
     gene            complement(69238..69459)
                     /locus_tag="ACHKNP_RS02355"
                     /old_locus_tag="pgaptmp_000471"
     CDS             complement(69238..69459)
                     /locus_tag="ACHKNP_RS02355"
                     /old_locus_tag="pgaptmp_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4702699.1"
                     /translation="MATIPTPTHPEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDSRNSLSYWCLPVNASAQEGL"
     gene            complement(69478..69597)
                     /locus_tag="ACHKNP_RS02360"
                     /old_locus_tag="pgaptmp_000472"
     CDS             complement(69478..69597)
                     /locus_tag="ACHKNP_RS02360"
                     /old_locus_tag="pgaptmp_000472"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HGU4702700.1"
                     /translation="MVVLAGLPKGRPVPFCAGSSNPVSATAIEIGTSRGSSLN"
     gene            69956..70306
                     /locus_tag="ACHKNP_RS02365"
                     /old_locus_tag="pgaptmp_000473"
     CDS             69956..70306
                     /locus_tag="ACHKNP_RS02365"
                     /old_locus_tag="pgaptmp_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702701.1"
                     /translation="MSSEHVRKGVTNAKFNEEQSNILFIEIGILSILIGLMSKSWWAF
                     GGSFLGLIFSLRIKFLAIPLMIVFSLVWGAIGYSIGALFESTAASIVLGIIAFLSGLG
                     THFAAVQWANDIAE"
BASE COUNT        17996 a        17389 c        16329 g        18706 t
ORIGIN      
        1 cccggatgac ttatcgttcc cgccgctgac tttgacatgt atttaacctt caaatcctca
       61 atgacaatca ttgcgtggtt tttgctgatg atcgttgtga ctttatgaag gtagtctctg
      121 cggatatttg cgatacgaaa atgcagtcgc tgtattttgc gtttctgctt ctgccagttg
      181 ttgctgaatt tgaccttgcg gcttaactgt cgctgaagtc tcgccagctt cttctggttt
      241 ttctgaaaac tgtttacagg ctcaaagact gtgccatctg acagcgtggc gagtttagcc
      301 acgccagcat ccagcccgat cattgatgct gaagggtgaa ccggagtgga tatttcactt
      361 tctgtctgaa tactgatgta ccacttaccg caggactggc tgacagtgac atttttcaca
      421 actcccgtga cctgacggct attccggtag cgcatccagc ccagtttcgg cagaaaaata
      481 cggctgtttt cctgatcgag cttaacgccc tgcgggtagc gaaatgcatc attctgtccc
      541 cgctttttga atcggggaaa agcagcccga ttctggaaga agtttttgta agcccgctca
      601 aggtctttca gtgactgttg caatggctgt gagggagaat ctataagcca ttcggtttca
      661 gtgtcttttt tccactcaac caaccaggaa gccattttcg tgtaagggat gtatttttta
      721 ccggcctcat gattctcatt ctgaagcgcc agagcacggt tgaaaacgaa acgacaagct
      781 ccggcaaagc gcctcatttg acactcctgt tgaccatctg gttttaaccg gaatttgaat
      841 gacacctgtt gtttcatgtc actattttaa gcaaaaatag ccgggaggaa aactgtgcct
      901 tatatcccca ctatgaggag cgtagggggc ggcacattgg ataaggcgta acagggatga
      961 gcttaagcga tcaggtggta atggccacca gcatagaaac gctgatcgag ctgctaaaga
     1021 acctgcccga ttatgggcgg gtttcgtatg tggtgacagc gaagggagac gaggtaaaaa
     1081 cagcgtttga tatcgtcgat gcctcagctc ttttggtatc caatactctg gatgggaaaa
     1141 ttaatccaga ctatccccag gaacttcagc cgcgcgaccg gacccgcgca tccagccttc
     1201 ttcaggtcaa ccagatatcc aaggatttgc gtcctgccca gcttaccgat tccggtttat
     1261 ccagccatgg cgcgccaata attggtgagg acaatgccgt tgagtcaggt aatgggcgga
     1321 ccatggggat cattaaagcc tatcaggacg gtaatgcgga tcggtatcgg gagtacctga
     1381 ttgaacatgc gaccgaattc ggcatacggc ctgaaaaggt tgaatcaatg acggctccgg
     1441 tactggtgcg ccgccggtta acgacggttg accgtgttca gtttgccaag gactcaaata
     1501 tttctgatct ccaggaaatg gcagccagtg aaaaggcttt tgttgatgcc gacagcataa
     1561 caccggcgat gatggcgctt tttaacccgt cagaaagcgg agatctgctt agccgcagta
     1621 atgacgcgtt tattcgcgga tttatgacgc aagttggtgc cacacaggcg gctggccttg
     1681 taactgaaga tgggcgacca acacggcaac ttgttgaccg tatacaaaac gcgatctttg
     1741 ccaaggcata taaggatgcg cgcctggtaa ggatggttgc agaagaacct gatccggata
     1801 tgcgtaatgt tctgacggcg cttaatgcgg cagccaatga ttttgtccag atgcaggctt
     1861 tatcaggaga agcgcacaag caggctgtga caactattgt tgatggcatt gagacagcgg
     1921 atagcctcga taaaaaggcg ctggcggcat tgaaagatgc ggtagacctg gtaaggcaat
     1981 cgaaggagtc aggccagcat attaccgatg ttattgctca gggggatatg ttcagcgaaa
     2041 cggccccgga ggtgaaagca ctcgcgttgt tcatcgtcgc gaataaccgt agcgcgaagc
     2101 gtatggccac cgcctttaag ttgatggcgc aacgtatcaa tgatgagtta cagcaccagg
     2161 gccaggcgct cggggatatg tttggcggcg gcgatgtgtc gttacaggat atccttcgcc
     2221 aagtatctca ggaactggaa aacgaaggta tgcaagggat atctggcggt cttttcgagt
     2281 cagcttctgg cggtagttac aacggtgttg ctccatatac cagcttgcta ttacatcggg
     2341 catccggcat caaagacatt attcatctga tcaggctgct ttcccgtaca gatccccagg
     2401 atgaacagct tgttcaagtg cttgcgcatt ttgttcgaat gcctgttgcc gacgtgaaaa
     2461 aatggtgccg attattcggt atcagcaatt cgttacttcg cggcttgtta aatcacgcat
     2521 cctcccttgg gcgcgatggc tttgacgaga tagcgcaggc gataaaaaac ggagatatgc
     2581 caccagctat tgactggttt tccattcgcc caaccagggt gaaagcattc cttagcgcgg
     2641 cgcattcggc atcaccattg gcagaaatgg ttcagaggtt gtcgctcata ttcacagacc
     2701 ataccgcgtt gggtgatctg actctggacg agatgaaaga agcctccatt cagtgggccg
     2761 atcaacaaaa tgaggttaac tcagacttct tgccagcatt caggaaggcc gttagtaaag
     2821 cggatgatgc ccgtggaatt ctgaaggcat ttaaggaatt gcaaagtcgt gttaataaac
     2881 atgtcggtga tatcgatggg gtaacggcgg aaggccggga tatccttaaa gagcacggca
     2941 taacgccaga gtttattgat gagatcagga cggatatgca gcgtgaggtc gtatcgtccc
     3001 tgcaaattgt agccagagcg ttggcggatg ctaatccgaa gagtgcggcc attgttaacc
     3061 gggttattgg tgatattgaa gcatcggagg gcatgggggc gctgaaactc ttcctttcgc
     3121 gagcgtttaa tcctaacggc aatattctcc ctggcattat tggtgaggct aaaaagtatg
     3181 tcagtgaaga agaacttgag cagcttgacc aactacttaa gcgattctca tataacccgc
     3241 agacacgctg gcaaatgaat cagcgaagta tgggttcggt ccacgagaaa gtgttatctg
     3301 ccatgaacag tgcgatcgca aactcatccg tatctgaaga aaaagctctt gagtgggccg
     3361 actcttttat cacagaagaa gtggaagaag cccgcgctgg acagaatggt gggatagacc
     3421 tgcgcaagga acttgctgat atttatcgcc tgaccggcgg gaaaatatcg accttatcaa
     3481 aggtagttca ccaccaggga agggcatatg caaatctaaa tggtgttgtt gctgtcaatt
     3541 tgaacgatga aaatgcaagt gcactgtggc acgagctggg tcatcatctt gagtacagta
     3601 accctggttt gttagagaaa gcccggtcat tcctgaaggc caatgttgaa ggggataagc
     3661 catctttcgt caatatcggt gggcgtggca agcctgaatg gtgcttcaga tctcgattga
     3721 gtaatattta tatggcgaag gtatacccgc cagcctcagt aagtaacact gggaaaattc
     3781 ggcagaaatc accgactatt tccaaaacgt cagcaacgga agtattctct atggctcttc
     3841 agttgtatca tgacaaagag gccgctgccg catcactgat gaatggtgac ggattgctgg
     3901 aactgttatt aggtgtggca aaggagctaa ataatgcaga ttaaaatcgc agcgccatta
     3961 ggcggagatg ccattatcga atttgatgat aatgaagaag tttccgggcg tttaagcatt
     4021 atctccggtg acattaccga ggacatgatc gctgaagcca tagctggggc aaatcccaat
     4081 agctatatgg gattcgttaa cacccttgat gctcccgcaa gtgatgttct ccgaacgctg
     4141 catctttacg ctggctggtt tgttgattgg ccagcagtag agggtggcga tgaggacgac
     4201 gacgatgatt ttggtgatca tgtagaccag atcgtatatt gaagaaatcc cgccgattgg
     4261 cgggatttct tgtttatgcc aggcgggcta tgcgtatttt acaatcatct aggtggatgc
     4321 gtagtgcttc taaacatgga gtaaagttag ctgagaattt gtcggcctca caggccaatg
     4381 cataaaattt ttcagctaat tcaagagatt catcccatat ttttcgactt acaaagtctg
     4441 gtagtgctgt ttttctaagc tcattagaca ttgctccccc agagcgtggg gcaaaaccca
     4501 taggtaacat gtcataggct ggagcaatat gatatgggcg tccgtggcta ctaataaacg
     4561 ataggttgcc attatgcata tcggtattgc ctattagcac accaaaagac catagccttg
     4621 ttgctataat aatcgcatca ggatggacgc acttattttt taccaactct ttaaccagca
     4681 agggccatgt tgctcttgca ttaccaacaa attctgcatc aagtgcacga agtgagaata
     4741 cacctacccg accgagttga ccaacacgat caaatcgggg aatttccaag aatctctgac
     4801 cgttataatc aaaaacttct gtttctaccc ccagaacttt taatgcgaga tgttcagcaa
     4861 acaagagatc tctccagcgc tcacttatag gggtgtcgtt aggcgcagag aatttcacta
     4921 atacatgtcc acgctctgtg aaagtacaaa atttgggttg ttcacctcca gcagatgaac
     4981 ctggcacatc accttcacca gcggctaaag ctaatacagg atattcaatt tcacgattaa
     5041 caggaacagg aggctgcatc tctaaaaaat gcgtgcgcgc ttgctctcca attaacaaat
     5101 taccaacaac atcatgccca tgcttaagta aggctttgat cacatcagta tctgaccatt
     5161 gctctggatt tgagggaagg ttaagttcaa gagcatatgc tgatgcatat gctcttccaa
     5221 ggaagccttg tggacgcata tcaaataacc accaaggtaa cccatcgcta tgcctgttat
     5281 ttttcccatc ctcaaccata acatagcctt ctggcctcac cggaatgaga gtaccaagaa
     5341 cgcaaataca cccctcttca gttatacgat aaatggggac tgattcgaat cctctaaaag
     5401 agtctcgtaa agcatattga atagatcttc catttccgat cctaacgatt tcatcaccaa
     5461 gttctcttaa tgctctagat atcgttggtt ggctaacact gagtttttca accaattgtc
     5521 ttggtgtcat aggcccttga ttgagcaagc tacggattgt tgttgcatgt gtagccatag
     5581 ttttaattac tgttgtgaat agattaatga ataggtttat gaatgcatac catagcaagc
     5641 aaaacgggag gtgtcactaa tttagataag ttaaaaagac ttctttaaga gataatgact
     5701 aggcgcttta actgctagct aacataataa ttggtcattt aacgtggagt tccccgccaa
     5761 ctgacggggt ttttattagt cactttctgt ttcttctgga atgttttctt gcggttctgc
     5821 caatgtagca cgacataggt tcctggcatt ggctatagcc acacttttga cttcatccgt
     5881 catcgtgcag gtaatgtact gatcgagctc ttcagctcgg atgatgcttt tgccaattag
     5941 aaactgtatt tgccagagca gatcggcatc cataatcaga atttctgccg ggccttcagg
     6001 gccagccggg aaggaaacat aagactgttt gcctaggccg ataactcgac aacttgcttc
     6061 aagaattgcg cgcttgaggt ctgactttat aacggaaaca ggttgatttt caccagtgat
     6121 tacgccgttg acatggaaag gcatgtagct tgaaatacgc tccactttcc acacgccagc
     6181 aagcgatcct tcatgcagca caatgggggt aaccgcgagt ttcacctcac catataactg
     6241 ctggcagata gctggattgc tgaatacatc taaaggctca cattcaaaca gcggcgcaat
     6301 ctgcatgagg tccatcatgg tcatccctgg ggtacgagca gtaatgaatt tgcgcatacc
     6361 agtatccatt gcgctccaga ttgctacacc atgctttttg ctcacttctt cagtaaagcc
     6421 aaggtggcac atgatggttt tttcgatagc cagatcggag atagaaacct tttcgccagg
     6481 tacaccatca ttattgatgg tcacttcgac actctggcca ttacgcaggc ggtattgaat
     6541 tgttttagta ttttgtgctg ccatagtctt ttctctgctt aaaaactgat gtattgcgcc
     6601 ttcaggtggg tcaggaatgt tttcccacca gcgaaagcaa tatctcgggg tgttctgttc
     6661 gttaaaaacg cgttccattg ccaactttgg cgttttttag cgagttcgtg cttttgttgg
     6721 cgtttggacc accgcttttc tttcagtcgt tttttactca taaactctaa aacggaatat
     6781 cgtcttcaaa gtccattgga ggttcgttat tggcgctgct ctgaggtttg ctaccaccgc
     6841 tgtattgctg gtggttttga ggttggtttg actgccccca gccatttgag gactgtgaat
     6901 cgtcgcggcg agcgccgatc atttgcatgg tgccgccctg gctgacgata atttccgtcg
     6961 tgtaacgttc tacaccggcg tcatctgtcc acttacgggt tttaagtttc ccttcgatgt
     7021 agacctgaga accttttcgt aaatactcac tcgcaatttc agcaattttt ccgaacaaaa
     7081 cgactttatg ccattctgtt tgctctttct gttggcctgt ttgcttgtcg cgccatgatt
     7141 cattcgttgc gatgctgagt cttccgactg ctccgccatt tggtatatac ctgatatctg
     7201 ggtcttgtcc cagggtacct atcaggatga ctttatttac accgcgttgt gccacttatc
     7261 tcacctaata aaataaatta attagagcaa taatgtatat ctttgaaacg tagctaacaa
     7321 gtgatttgca ttatcctgtg ccttctaaag ggatcgagtc agtcggtatt ggctgtgaat
     7381 gggtgtttgt cctggagcgt aaaaaattcg cttatgaggt ctttatgaag ggaaaaacag
     7441 ccgcaggagg cggtgcaatt tgcgctatcg cggtgatgat taccatcgtg atgggtaatg
     7501 gcaatgtgcg aaccaaccag gcggggcttg agcttatcgg aaacgctgaa ggttgccgac
     7561 gtgacccata catgtgcccg gcgggtgtat ggactgacgg gattggtaat acacacgggg
     7621 taacgccagg cgtgcgaaaa actgaccagc aaatcgccgc tgattgggaa aagaatatcc
     7681 tgatcgctga acgctgtatt aatcagcact tccggggcaa agacatgccc gataatgcct
     7741 tcagtgcaat gacaagcgcg gcattcaata tgggatgcaa tagtttacgg acctactaca
     7801 gcaaagcgcg aggcatgcga gtagaaacgt ccatccacaa gtgggcgcag aaaggggaat
     7861 gggtgaatat gtgtaaccat ctccctgatt tcgtgaacag taacggcgtg cccctgcgag
     7921 gtttaaagat tcgccgtgaa aaagaacgcc agctttgcct gacgggactg gtcaatgaat
     7981 aaactccggc agctccgccg actttcgaca atgaagttat cgctggcggc gatagttttc
     8041 gactcgattt tcatggcggt atatgtgctc aatgagacgt ggccactgga accgctattg
     8101 tatgccgggc ttcggctgtg cctgacattt ttgagcatgg ctgcaagatt gatgcagcag
     8161 aaagaaaccg cttcagattg tccacgccgc gcggtgcgca aatatatggc acgcaggcga
     8221 agatgattta accattattg ctctaataaa tttgatttat tagagcaaat aacctaccat
     8281 caaacacggc tctattgatc tcatcgtcgt tcaggcttat agttccaccg tcgtagcaaa
     8341 ttctgcgacc gggtttagca gcctgaatga ttgtgcggac agccgcagat atccgatatt
     8401 gcggtatttt tgtgtccgta aaaccgcgtt acgcccaaat tatggtgggg cgtgatgggg
     8461 aggcttcggc ctgctggttt cacaatcgcc agtctgctaa ccccgtcacg tcctgccacc
     8521 tgtttagcag cgggtagcag gttgttaaac ctgattgtga ggccgtaact atggttaatg
     8581 ccaatccttg cgcacgccca gaattcatct ggcgtttcta ttcctgtaaa aaacaccact
     8641 atcacttcgt tatcgcagca actgaagacg aagcacgctc tcaattgcct gatggtccct
     8701 gcatttttac tgcccgtttt tctactaact cgcgcaattc acttagttac tggaacctcc
     8761 ccttctctgc tgacgttcag ggggttttat gaaaaaacct ctcgtcaccc gtaatgaaat
     8821 agccgaagcg atcgccctgc atactgcctg tatgccgaca cgggagatcc ccggcgcaat
     8881 tgccaactat ttcatgataa ccaggcgttt ttatacccga acagataagg ctgtgatcaa
     8941 caggctactg atagccgaga tcagggatta tttgattgaa caaggacgtc tacgttacgc
     9001 aacggtggca gcagaaatga gaaaggaggc acatagaatg accagtaata atttgaatgt
     9061 taaaaaacca gcacctgttg cttcagcaac gtcagcacca gccgtgaatg tcatccccac
     9121 caccggagac acaatcgaca gcctgacact gttaaagatg gtcaatgaag cgcgtaagtt
     9181 atgtggggaa ccagaggttc ggaacaacaa attcatcgaa aaaatactcg acgaattaga
     9241 aggtgaggac ggttacacaa aaagtgcaac cgtgccgcca ggtggcggta cgcctatggt
     9301 tgtcataacc atgacctaca aacaagccct gcgagtcgcc gcgcgcgaat cgaaagcggt
     9361 ccgccgttcg ctgatcgaca aactggaaga attgcagcag gcaaactcac ctaccccatc
     9421 gatcccccaa acattaccag aagctctacg cctggctgcc gagttggcag aacagaaaat
     9481 gcagttggaa caacagctgg tggccgcagc ccctaaagtc gattttgccg accgggtatc
     9541 agcggctaat ggaatcctga tcgggaactt tgcaaaggtc gttggactta agcaaaacgc
     9601 ccttttctca tggttgcgcc agaacggcat tctcatggct tttggtgcgc gcaaaaacgt
     9661 accgcgccag cagtacatca acgccggata tttcacggtg aaagaagtgg tgctggatga
     9721 tgaaaatggc taccagatac ggttgacgcc tcaattaacg gggaaaggcc agcagtggct
     9781 gacgcgtaaa ctgctcgatg caggtttatt aaagcctgta gcggctgagt gattaaatta
     9841 aaaaggcggc ctttcggccg ccagtgattt cagatacccc ggcagccgcc ggggttattt
     9901 ttggtggtca attaaacgga ttgattgaat tattaaacgt gatgatgctt gtctcacgcg
     9961 gtgcctggac gttagccgct tgcggaacct ctttaatttt cttagtgaca ggcaagttgc
    10021 gcgcgccaac tttgatcaga gattcgaaaa gtgtggcaac gatttttgca tcaccaggtt
    10081 ctttgaggcg gaatgcgtct ttttgggcgg cggagacgaa gatcgggagg ttatccagtt
    10141 cgtcttgcat tgctgccagc acatcgttgc ggatacccgc tgtttcctcc agcaaagcaa
    10201 ttcgcgcttc agcatctgcg atcttggcca ttgcttcgag gtggcggccc tggctttcga
    10261 gtagtgcggt ctccagttct gccgtacgct ctgtcgcctc caccatcatt tccagttcag
    10321 ccattttgcc gtaatgggat ataacggcct gcactgactc gtcggagtac ccatgcgccg
    10381 ccagggactc tgccagtaaa gatttagaat ccgcgctttc aaacattccg gcgctggcag
    10441 gatgatccag acttatatag ttcggcgttg tcacataatc cacaccatgg aagctggtgg
    10501 ttacagcgat tttcccggac tcgcgcccgc cagtggccca gctccagcca ccagcacggc
    10561 tttcgatcat cgcggcgacg attttccccg gctctgtatt aagaatttcc tgtgtatggg
    10621 taacgatgcc gttgtcgtca acagatatag ccactgttcg gcacgctgga acattgtcga
    10681 ttacgaccgg gcgaccttcc accatgatta cgctggtttc tggtacttcc agtttgccgg
    10741 tcagctgtcg gcgaccgtga ccgtaatagc cgaaaagctc accaaggcgt aaaccttcct
    10801 gagtttcctt gctttcaagc atggtcttta ccgcgcttaa tacatactgt cgcccgttct
    10861 ggcgaccttt tcgagcattg ctatagagac aaaagcggtc agtgaccgtt ttcaaaacat
    10921 cagtcattat cgtttccctc tttaaagacc gattcaagga tttgcgccag ttcctgtggc
    10981 ggtgttttga tgatggaatc catcaggtga tcgtcgtcct cgcttttagc tttcagttcg
    11041 ttcaccagtg cttcagagat tttttcgtca atctccagca catcgctgaa caggtaacgt
    11101 ttgaatgcat cggaattagc gaggacgctg ttattgctga cggcatcgag gatttgcgta
    11161 acgatggtgg cgtagttcgc ctgcgagtcg cggttatcgt tgtgctcttg ttgcagagcg
    11221 gtattaacgg agtggaattc gattttgtac gggcgatcac cttccgggta taccttgccg
    11281 tacttgaaag caagatgaat atcgatagcc cgctgaatga actcttctac gccctgctgg
    11341 atccatgagg cgcgcatggc ggcctgaatc gccgtgcgca ggaatccacc ttcaccaagc
    11401 ccgccggaca tttgatctgc ccaccccagg agggtgtaat cgaggccaag tgctgccgcc
    11461 agctggcgca tataagtgag aatgtcttca atgccgttga tgtctgcctg gatggtctga
    11521 gtatcaatgg tcatctgccc cttgccgtcg cccataatag gcagcagggt attggtgacc
    11581 gtaggcatgt tattcgcgcc gcgtgcgcgc ttttccatca ggtcagctgc tcgtttaagc
    11641 gtctgagtaa tggtgcgtga ataatcggct gcttttaccg gatccagact attcatcgcc
    11701 agaccgatga ttcggtcaat tttcgacgca ttaaaacgcg ttgccttcag cgagcggatc
    11761 gccgaacgca ggttcatgta cggctcgtag gcgtattcga gcaagctggt cccgtaattc
    11821 tgggtttcaa tcggcgtgcg ctcttccgga ttatccagca ggctgtaagc cttatggcca
    11881 gtgtgcacag gcataaggtt tgacttaggc cgccagtagg gggttttcat agggataatg
    11941 gtccacggat cggcgaaaac cattttccct gacgcgtcct tcagataatc gccgctaaat
    12001 cccgccaggt taccgctgac ctcgaactcc ttgatgaagc tcggaagggt gtaataggag
    12061 cactcaaaag acgtgatccc tatgccttct ttggcgtatg gcctgacata agccacccca
    12121 aatacagaca tgataaatgc ccacccggcg acctctttgt tgatggttcg cccgatgtcg
    12181 ttcatcagct cgtcacacaa cccctgcgcg gcgtcatagt cactatcgtt tccgttgtgt
    12241 accggcacga tagagaaggt ttgtccggtc ttcttatcga aagagagcgc gtgcgtaata
    12301 tggatgttca gcgcggtggc gatcgtgctg taaaccgcca tctcttcgag tagcggatag
    12361 cgttgcaagc ggtcttccgg cagttgaact tcatcaaaga taaagcgact tccgtccacc
    12421 agcccatcgc cagccatgcc actatcgccc ggtttgccgc ctaagaagcc ggacagttgt
    12481 accggtgccc ctgcgcgaga aaacaaatac ccacttccgc cgtgcacagc cagcgcggac
    12541 aggaggatgt tgtcccgttc tccgttgtct ttaaaaaccc ccgccagcgc cttcctgacc
    12601 gaggatagcg tgattttatt gtctgccaag attgcacctt aattagaata attcgcatcg
    12661 tgtttgaacg gaatttaaca ctagtcactt gttaaggatt accaatgaac aagctatcta
    12721 tgggggtgtt tcgctgttca agtgtcagcg aaatattgaa atatattagt gcaataacat
    12781 ttcatcgggc gccgattaga tacggcgtgg aaaaggtgga aggcaaaagc tatgaccgac
    12841 tacgccggac gtgaaataat atgtccacac tatctgatac aataaaaccg aataggacat
    12901 aatttcgacg tcaaagcctt ggcacaggct ggagagaaaa cttgaaaaag cgatactaca
    12961 cagtaaagca tgggacgcta cgagcattac aagagtttgc tgacaagcat aacgttgagg
    13021 tgcgaaggga agggggcagt aaagctctgc gcatgtaccg tccggacggg aaatggcgga
    13081 cagtcgttga tttcaaaaca aacagtgttc cccagggggt ccgcgatcgg gcattcgaag
    13141 aatgggagca gatcatcata gataacgcat tgctcctgaa tgcagattaa actttccgcc
    13201 cttttgcccg gtctatcgcc gggcatcttt cagagataat tacctttacc cgtcaattcc
    13261 ccttgtggta cttgtttttg cgccagtgtt tgggttccgt gaattatgtt aatcagaggt
    13321 cttagtaacg atggttcctg gcgggcctca acttctccag ccattgccct gatgtagtcg
    13381 gcgctggcaa cattgttgta ttccgttgcg aaacagcata acaatgtcag aacatgctct
    13441 gttgttattt cgctccagtt gatgttgaaa aattcatcgc cttttttatc gtgttcggaa
    13501 tcgaagatgc tttggtggag gatgtatttg ccggattcct tgcgcggtaa cttgatcgct
    13561 ttctggcgtt ccagctcctt gtaaatctgc attgcttcaa ttagtaccgg cctgccgttc
    13621 atgaagggat cgcgcaacct tacacgctgg ccaactcgac cggtaataaa gctgttttcc
    13681 tcttccacca gcacgataaa acccttttcc tctttttctc gcaattcgcg cagcagctgg
    13741 agttccatat cgcggcggcg ttcagggtag ctggtccgct cagccatgat cagctcattg
    13801 ttgatccatg cagcagtcat tgacgccggt ttgccgacgc tcattgaaac aacgcatatt
    13861 ttcttatcca tagcgcccct acaaaaaaga aaagccacca gcggcggctt agcaatacaa
    13921 ctgaaggtag cgcccggtac tcagactgtg ccgtccatgg aatatttgaa aagggatcca
    13981 tccgtaccgg gcgtgtgatg attctgactg aagtcacttg tcagttgtca atcatttatc
    14041 attaaaaata atatatttat tagtgcatga tgtttgccat ctcataggca tcagccagta
    14101 actccatctc tgacttgttc agcaaggtga attctttctt gcctccaacc acaccatcgg
    14161 catgaacagg gaccagccat gggtattttt ctcttacttc agccggagct gcatgctggt
    14221 ggtgccatct acaaagtggc aattgctttt tatgacaacc cggcgcggta cgaccggcga
    14281 tatggtgcag agacacctca ttagatatta ctccatgcat atagcaggca atgcagggga
    14341 gagcgccaag agcattggcg atgcgccgtt cctccgccgt tggtgttctc cccttcaagc
    14401 cacgagattt tatttttacc gcgcttttcc gcgttttgct ggctggtggg cgctctttct
    14461 gtttagcgat acggcggtcg atagaatccc gcattttctg atattgcgat tctctccagg
    14521 cggggtcagc caacttttcc cgttgccgag cgatcgctcg ttctctggct gccttctgcc
    14581 actcgcggcg ctgttcaagt ttttgttcga ttgttttcat atggcaaaaa aaaggcggcc
    14641 taatggccgc caatgatgtc aaggagttaa gtaatggcaa cgtcttcgta gttgacaaaa
    14701 actgcggctc aattatagca atcaattaga gcaatggtag atattttgtt aatcgagaat
    14761 cacatttttt cacttcagta cctgtgtgct atactctttc ttgattgatt ggatgcggaa
    14821 tacaaacccg ctcttttgtg cagcctggct ccttgccagg cttttttatt tcatcatgga
    14881 agctgttaac gctttggatc ttgctgaact gattgaaagg gcattgttta ccttacccag
    14941 gagttcgcca aattccgcca tcactctagt aagccctcgc cgcgcttcct cctccgttgc
    15001 attcatcaca aaatgttcag cactccgcat gcttttaacg gggaacgcaa cagatatcga
    15061 gtcgatatca ggcatcctat cgctcaattt tacggtgaca atgacagatg gtgactgaat
    15121 ttgagagctt acagacagca ccacatattt tccgtctatt ttgaaatcct tccgcatgtg
    15181 tcaccataaa tatcaaataa ttagagcaat caagtaaaaa tgaacggcta atcgccatct
    15241 tccagcaggc gcaccattgc ccccgtttca ctatccaggt tacgaatgta gttcatgaca
    15301 atatttacgt tggtccagcc accagcttgc atgatctccg gtattgaaac tccggcgcgg
    15361 gccatatctc gcgcggcacc gacacgggca ctatgtccag accaggccag gtatcgctga
    15421 ccagagtcat cttttgcccc gtaaatcaat cggtgagttg cttcaaaaat cccttccagg
    15481 gcgcgagttg atagctggct ggtggatgat ggcgcggcaa caccattttt tctgacgcgg
    15541 caaaacaggt agttattcgg atcatcagct acaccagaga cagaaatcca tcgctcaacc
    15601 agtttagtta cccccaggct aagtgccttc tctacaccag cggtgctaac cagcgttttc
    15661 gttctgccaa tatggattaa cattctccca ccgtcagtac gtgagatatc tttaaccctg
    15721 atcctggcaa tttcggctat acgtaacagg gtgttataag caatccccag aaatgccaga
    15781 ttacgtatat cctggcagcg atcgctattt tccatgagtg aacgaacctg gtcgaaatca
    15841 gtgcgttcga acgctagtgc ctgttttgca cgttcaccgg catcaacgtt ttcttttcgg
    15901 atccgccgca tgaccagtga aacagcgttg ctgtcacttg gtcgtggcag cccggaccga
    15961 cgatgaagca tgtttagctg gcccaaatgt tgctggatag tttttactgc cagaccgcgc
    16021 gcctgaagat atagaagata atcgcgaaca tcttcaggtt ctgcgggaaa ccatttccgg
    16081 ttattcaact tgcaccaagc cgcccatgac cggcaaacgg acagaagcat tttccaggta
    16141 tgctcagaaa acgcctggcg atccctgaac atatccatca ggttcttgcg aacctcatca
    16201 ctcgttgcct cgaccggcaa tgcaggcaaa ttttggtgta cggtcagtaa attggacatt
    16261 tgacactcag ataatggttt taagtaaagt gtacaggatc ggctctgcct ttacctgttt
    16321 atgattctcg tcatagaaac gccaacgacc gcgactgcgt tctatctttt cttcaccgcg
    16381 cgataatgac agttgacaac tatcacgatc aaaccctttt gcccgccagt aaccacgatt
    16441 tttctcaagc tcaagatgag tggaccttta gcagctgaat atcccatttt tcacctctga
    16501 ttgattggtg gtgctaagtg cgctacgcga aatctggagc actaacactg ccaacatttc
    16561 gcagatttta cgtagcgcaa ccttgatcaa atgatcaagt gatcactatt tgacctgata
    16621 aggtattgaa ctgtatggat ttacaggtaa attaatcatg ttcaataacc cttaagataa
    16681 cttcgtataa tgtatgctat acgaagttat taggtccgaa gaggagttta cgtccagctg
    16741 cgcataaaaa tcaagaatta ttagagcaat aaattttgag agaaaaatcc cactccacca
    16801 gccaaaaact ggattgtttt tcatagttgt ttgacaattg ctctaataaa ttatagtttt
    16861 gccgccgttt cgtaatacga ctttggattc actatttaat gtgtcttcag cgttgtagag
    16921 cggctcagaa ggaaatgagc aaacagggaa accttataca acggcattac agctatgcat
    16981 tgctcatctt acacacagcg caatgttgtt agattacccc agcatggatc atgggtgaaa
    17041 cagtaggtca gagcttcagg ctctgtgttg tcaatacagt gaggcataat tatggctttc
    17101 attccaccaa ccatcgacga cgttagacat tgctctaacg ctttatctgt agaccccgcc
    17161 gaaaccgacg ctgcccgcgc cattgctgaa cactactcaa agatatccaa tcaggagtac
    17221 cgcatcaccc aagacgacct ggatgatctc actgacacaa tcgaatatct catggccact
    17281 aaccagccag actcacaata aatgcactaa taaatctatt attttcgttg gatccttcta
    17341 taatggtggc caacaactcc cagtgtaatc cgctgtgagt tgttggccat gtcaattctg
    17401 gaggaggatc aatgataaat tatgtctacg gcgaacaact gtaccaggag ttcgtcagct
    17461 tcagggatct ctttctaaaa aaagctgttg cacgcgccca acacgttgat gccgccagcg
    17521 acggtcgtcc tgttcgcccg gttgtcgttc tgccgttcaa agaaacggac agcattcagg
    17581 ctgaaattga taaatggact ttaatggcgc gggaactgga acagtaccca gacctcaata
    17641 tcccaaagac tattttatat ccagtgccta acatccttcg cggtgtgcgt aaggttacga
    17701 cttatcagac agaagctgtg aacagcgtca acatgaccgc tggccgcatt attcatctga
    17761 ttgataagga cattcgcatc cagaaaagcg cggggatcaa tgagcacagt gcgaaataca
    17821 tagagaacct ggaagcaaca aaagagctaa tgaagcagta cccggaggat gaaaaattcc
    17881 gtatgcgcgt acacggcttt agcgaaacaa tgctgcgcgt ccactacatt tccagtagcc
    17941 ctaactacaa tgatggtaaa tcagttagtt accatgtgcc actgtgtggc gtgtttatct
    18001 gcgatgaaac tctccgtgat ggaattatca tcaacggtga attcgaaaaa gcaaaattta
    18061 gcctttatga ctccatagaa ccgatcatct gcgaccgctg gccgcaagca aaaatatatc
    18121 gcctggcaga tattgaaaat gtaaaaaaac aaattgccat cactcgcgaa gagaaaaagg
    18181 ttaagtcagc cgcatcagtt acgcgcagcc gtaaaaccaa gaaggggcag ccagtaaacg
    18241 acaaccccga aagcgcgcaa taaattatgc ccggcatcaa ccgggcattc ttccattatt
    18301 cagctgccac cggttttaac aagccagcat cgagcagttt acgcgtcaac cactgctggc
    18361 ctttacccgt taattgaggc gtcaaccgta tctggtagcc atcttcatca tccagcacca
    18421 cttctttcac cgtgaaatac cccgcgttga tgtactgctg gaacggcaca tttttacgtc
    18481 caccggacgc tatcaggatg ccgttctccc gtaaccagac aaacagcgcg ttttgcttaa
    18541 gtccaacaac ctttgcaaaa ttcccaatca ggatcccttt agctactgat acccggtcgg
    18601 caaaatcgac tttaggagcg gcggccacca gctgctgatt tagctggtgg gctttctgtt
    18661 ccagaagttg cttttgttca gccagttcgg cagccaggcg tagggcttca ggaagcgtct
    18721 gggggattgc gatcggttgc tgttcttttt gtcggaagta gctgtcttcc agtttttcaa
    18781 agaatgccca tgcctgatcg gtttcgagca ttttagcgtg gcgggctgcg ccgcgttctg
    18841 tccagagggt gagtgagcga acgttgcgag caatttttac agagtagttt aaagctactc
    18901 tgtgcttcaa ctcgcgcaat gattctcctt caactttgaa aaagtgcttc ccttcaacaa
    18961 agcgtacttt gttctcatga tgattttggc gaatacggat tgtttctgtc ccataccctc
    19021 tagcaagagt ttcggttgtc actacacgca ctccctgcca ttccagaacg ggaatttcat
    19081 cagactgatt ctgaacaacc accagctccg attcctgaac tgaaggtgca tgaatttttt
    19141 ctgatttaac gttagttgct ttcattctgt gtgcctcctt gcgtgcttcg gctgcgacgg
    19201 ttgcgtaatt cagatgaccc tgttcgagca ggtattcgcg gatatcagac aacaggatac
    19261 ggtgaaccgc gttcttgtcc tttctccggt aaagttgttt ggtgatcatg aagtagttgg
    19321 caataacgcc tggtatatcc ctggtactga tacaggcagt gtgctgttca attgcctcga
    19381 tcatctcttc acgggtgact aatgacgttc tcatagtccc tcctgagcag aagcgttaac
    19441 agggaggcac cagtaactga gagaattgcg tgaatcagtg gaaaaacggg cagagaaaat
    19501 acatggggcg tcaggaagct gagagcgggc ctcatcttct gtcggtgcaa ttacgaagtg
    19561 atagtgacgt ttctggcagg agtaaaagcg ccagataaat tcaggatgag ttggggtagg
    19621 gatagtagcc atattggcag cctccttaga cgttggtatg taaccaccgc agaagagacc
    19681 aatcttgctg gcggtggact gtacggagtt ggccttactg gcgtccaagg taaccagcct
    19741 acccgaaggt agccccatac agcccaccat tgtagaggtg tgcgtgtacg ccgatacaaa
    19801 aaaagacgcg agcggcgtct gtatcgcctt agacttaagc gggaggccaa tcccggcacc
    19861 cgttttaatg aggtgcctga taagcataaa ccgaaaatgc ctcaaggcgc aagaggtcag
    19921 gttcaatgta acatcggtag ctaaaaaaca caatttatta gagcaaatat tcattcatta
    19981 agccatgcca gagcttcatc aacctgcgct tcgtcttcga cgctaagcac ttcatcctgg
    20041 ggaacataat cagccagcat agcgaaacaa tatgtatccc aatggtctgg tgagtgcagg
    20101 ttgagttttt tcttcatatc ctccttactc atcaccttcc attgacctgc ggagttaatc
    20161 cctacaggga ttttcgacgc ttcctcaata gtttcattac ccttatccag tctcatacga
    20221 ccagatttta cggcctctgc ggcttgaacg ttggcataag cacgtttatc aaagtacagg
    20281 ctcttatctt cacggctatg catcttttta ccccagcgta tacgctgtac ggtaatacca
    20341 taatactcgt acatcagatc cgccgttgct ttacccagcc catcgccgtc tatcgctatg
    20401 gtgatatttg ggaatcgctc aggattacat tctgcgaaaa ttttggcggc aagctgcgtt
    20461 tctgtaacgt ctgtgtattc cagcattcga tagttgatta cacggcgttt atttctctgg
    20521 ccggacacca tcatgatatt gataacggac ttatcccgtc ccgtaccacc agcaacgtcc
    20581 acacatgcaa gccagcccca tcctttggca atcttgactt tccgccgcgt cgcacgttca
    20641 acctcatcac gtccaagaag gaagccatcc tgtgatttag ggaatagtcc gcgtacctta
    20701 atcatgtaca tagggttatc acgcccgccg tactccgcca gcttcatttt gataaatgct
    20761 ggcgttacca gcggtgattc ctcactgtta agcgtgatcg ccgtataaac gccatcaggg
    20821 ttaccaggac gcttggccag tttatggtga gtatcgtaga aatagccgct tgggcgtgta
    20881 ggctgtgaca gtaataagat gcggttatcc tgtccggtaa gagcaccagt gatgataccg
    20941 aaagctctat cactgacacc ggaggcttca tcgataatat acagaagatg atctgcgtgt
    21001 tcaccggcga gagcttcttc acttcccaga cgaaagccct tcggtactac agtccataca
    21061 cctttaccag taacctcata gaaagcggtt tctgtcagaa caaaataatc agcaagccat
    21121 ggaaaacggc tggtggcagt agcccagttt atcttgatgt acttgaatat accggtcatt
    21181 acctgctgaa ttttgttcgc aacgataatg gcgcgggcac cgggatacat gattatgaac
    21241 aacatgatca tgatagaagt catgtctgat ttcccggtac cgtgaccaga cgaaacagat
    21301 gtcttgctac cctgttcctg cacagactca ataatcagat cctgctgcca ggtaggtgtt
    21361 ttgccgaaca aaacatcagc ggcagcaatc cagtcataac gatatagcgc caccagctcg
    21421 cgccaacgtg gatccgttac gcaacttctg gccattaatc atcatccccg tatagcttgc
    21481 gggtaacttc ttcgtcttcc tcctcgtctt cgtccaggtc ttgttccagc catgggtcgt
    21541 ttggtacacc ttcagtatca acatctccat aaccgcctgt atcaacgata tcggcgattt
    21601 cttccctacg ctgctcaatc cacaatgcgg catcggcgcg gcggttggcg gcccgttctc
    21661 gcgcaacttt gtccagatct tcaagagaag ggccaccgac ggctgtttgc ctttcctcat
    21721 catcggtatt ggtcttagga gcacgcagat cggctttgat ttgctccagc atcaggggcg
    21781 gcacttttcc gccatgcgcc tcgatgaatt cagctgcttc cagcactgac cagttatttt
    21841 cacgctttcg ttcgtatgcc agcttaacaa tgccagcttg ccccatagat aaagcgtgct
    21901 tttccgcctc ccggctttct tttcgatagt tattccggat gctgtaaatg gtgttgatca
    21961 ggctgcttat ctgcgcggaa cagctgttta gcatgctcgc gatacgatat tcaggcggag
    22021 tcccttcatc atcgtctttt tgctgatcgc gcatttcctg caccagacga atacacgtat
    22081 ccctggcgtt ctccagcata aggagatgag aaagagactt ttccagaaga gtggtttcca
    22141 gaacatcggc cccggaccga cgcaacatag cgcgcgcggc cttccgcgct tcaacgttat
    22201 ctatcaggta atcgccagct tcgaattcaa agcgttcacc atcatcatcc agggtgtcgc
    22261 gttccaggcg atcacgtaag gtccggtggg cgcgggtgat cacgtcatga tcatcagaac
    22321 gatcatttat gcgcttattt tggcgcttcg cattctcgac tgcggcactg acaacggcat
    22381 taaccctttg tttttccgtt atttcagccg caatgtgatc acctgcatgt tgatcattag
    22441 agtgatcaat gatcatgctt tttagtggct tcctgactgg cttatttggc ttgcggctgt
    22501 ccgctgtcct ggtgtcttct ttgaaggcac ggagataacg acgtgcagta ttagggttaa
    22561 gattaaactc ggcggcatac tgtgcgatgg tgtaaccacc atctcgcgcc aggcgagcaa
    22621 aattcttctt gtgatcgtcc caggtcactt atgcttcctt tcgtaaaaac tctttttgac
    22681 gcgagggtaa cgaaagtcac atgtcaaaag gcccggaacg ggcaagcaat caatcagata
    22741 cgtgcggatg tggcattacc gtaatgacgg tgctgacggg ccaccttatt gaaaagttga
    22801 cgcgccatta cccaaggctg gtgctcccgg cgttcctttt cgtcctgcgt catatagagt
    22861 tcgttctgga gtttttcatc aaaccggcgc ggagcgcggc tacggcgaaa gaattcagga
    22921 ttcagagagt ggatctgaaa tctacgtggg cgtgtactgt catcaatcaa aacagacgaa
    22981 tacttagaca cagcgatagc ctttaagcgc agataaacat cgcgcttatc gacatccaga
    23041 tgcgggtatt ccttttcaag aattgctgcg agttctttcg ctgatagaag agatttagtg
    23101 cggatcatgt aatccgcaat ctcgtacgat gttattcgtg agtgatttat ttccatgaag
    23161 tggcgtccct gccagttaag taacatcctg tcacctactg attagcccat gtcaactaat
    23221 caacgtggaa tataataccc tcgattaaag aaatagcaat acattagagc aattttatct
    23281 aacgctcgac gagtgacttg tgatagcgcc gactccaagc gcgtaatcaa agaacaatcg
    23341 ttgatgcatc gccagcctac cgtgcgtctt ctcccaatta tcgcggtcac gctcaatatc
    23401 acgctggcat gactggcaca gaggaattgc gtaaatgtca tgcgcgcata atcgactatg
    23461 acgaacgata taaggcgtaa tgtgagcgcc agctcccgca gctccacacc cacagcatgg
    23521 acgggaagca acaaagtcca tgtactcagg taattttagc gattgcagtt ttggtatttt
    23581 gaaatgcgcc atgccagggt cggagtcaac atccacaggg catacttttg cacgcatcgg
    23641 cgcggcgcgt tcttccatca tctgaacata tgctgtagcg cgatcgtcat acgggcgaat
    23701 atccgcctct ttcagaggtc cgctatcctg cgttgcggct ttcatcttat ttattgatat
    23761 acggcaaact tcttccggca tcaggtgcat catgttgcgc atgaaagccc accagcacag
    23821 ttcctgaata cttaaatcat ggccatctga aagccccatt tcctgacggg cgacatccag
    23881 tatccagtta acgcgattat tatgcagcgt ttctttcagc tcattaaaac cacgcatccg
    23941 gtaatggtta tcgtgatgcc agcacaacaa caccgcgcta ttgtctcgtt ctgcgtggac
    24001 aatatggttg tcacaccagc tacgatctgc ggcctggcat tgcccctctt tcctgcgcaa
    24061 ccacgccacc agcgcgtcaa ttccaccaat acggcgaaac agttcatcgc tgttaaaaaa
    24121 cggctgcaac gcctcatttg ttgccatagt ttgctcggaa acaacgaggc cgtcgtccat
    24181 gtgctcgatt aactcacgcg gcaccggctc cataataaat ttacggccag cctccaccag
    24241 ctttctgact tcctgatcca ctttgaatgt ggcgacgcca agctcttttt gtacaaaggg
    24301 agtaattacg gctttcacat cacacctttc atcactgatt gggctttatc tgctgcccgg
    24361 cattctctgt ttaagcacaa ccatttcctg acggcataac acagcaatag cggtcctggc
    24421 accaatttgc ttaccaacca ggtattgctt tacctcgcgg cgactcacgc catcaagaag
    24481 catctttaac gcttcacggg acaatttgtt gtatttacgt gccattaatc tactccgcgg
    24541 aaccatacaa tctacgtaac gtgtcggcga cagaagatac agatatctct ccggtcgcag
    24601 cccctacggt aaggtctgcc agttcaggtg tatcaaatac ctgcaccccg ttacggcgta
    24661 gaaataacag cgcactgttt agcgcggtac gcttattggc atcattgaat atatgccctc
    24721 tcgctgtagc caccaggtag gtggcggaga cttcgaaaag gtcgctgatc tcttcgtagg
    24781 caactctggc ctgaactctc ccgataatgg cctctgccct acccggatct gacattcccg
    24841 gcaggccacc gtagcggctt atattcgcat catgaagcgc aataagttct tccggtgata
    24901 tatgcctcat tatcggttaa ccagttcctt gttggtggag tccagggtgt caaacaggga
    24961 tgcaaattcg gcatccagcg ccgctttttt gtaggcttca aaagtagcct tgctaacaat
    25021 tactgctggc tcacggcctc tgcgggtgat ttcaacctct tccccggctt caacattgtt
    25081 gagcacttca gaaaggttgc cgcgcgcggt acggaagtta atggattgca taaacacctc
    25141 gtgtgctcgt tatgtgtacg caattatagg ttttaatgat gctgaatacc acttcttggt
    25201 gccttaaatc ctcgagatgc agtcttgcaa ttgctgggat taagcagaat aagtggttgg
    25261 aattctgagc agtaatccaa tcttggctat gtgctcatat ggcagctctc cggttttacc
    25321 gtcatgtatc gaggctatca gattcaattc ttgcgccgct ttacgaattt cagtttctga
    25381 tggaacaagg cgaaaaccga tgtgtaacat tttgatatag aaaggtaata gttcgtactt
    25441 cacccgtaac tgtgctgccg catctttcaa atcatggatg atttcttgtt ttaagtcaga
    25501 gcggggattc gatagctttg cttggttaga caataacaga tgcgatattt cccccaactg
    25561 ttccttgaat gaaatgtaag actcaacccc cttgatgatg agtcgaccta ggacaaaaac
    25621 gcttactcca gtgacaacag tgacaaaagc agacgaactc attcgcgtac ctaaaaagag
    25681 aattataacc tcatcttatc ttcaagtgag taaccgcgtc taatgggagt aatcatttaa
    25741 atggccaggg cgatatagat ccagtcatgc ctctctatgg tttctctgaa aacaaatcat
    25801 atagcaggac tatcatcaaa ttcaccgccc ctggcgtcat tgataatgtg ggtgatcaca
    25861 ccaaaaacag cattactgcc cgtgtatcca tcgtcatcta ctggtaacgc ctctttcttc
    25921 ccggtgctta aatcctccag gtgctggcgc ggatactttc ggtatctctt tatgcgatat
    25981 tcaccctcca tagcgcagac aagcagcgaa ccatcaaccg gagtaagcga ggaatcaacc
    26041 accagcaaag caccctgcaa tattccctca cggtgatggc tgtctgccgc ccgcatgaag
    26101 taggtcgctg atggatgcct gattagttgc tgatcaagag aaattctgct ttcaacataa
    26161 tccgccgcag gagaagggaa gcccatagcg ttttcacctc aataatactg ttcatttata
    26221 cagtatacat taaagagaca cctttggtgc aaactcgtta tgtacaccaa ccaccgcaga
    26281 tgattttgtg cgctttgcta ctattcatca ccaatggatc agcgtaatct cattgccaat
    26341 ctgcagaatt ttatccacca gcgatgtgat tttttctgcc aggtcatcat tacttaccca
    26401 atcctggcgg gcagccagaa cgggcgacaa ggctaatatt aactctcgtc gtaatgatac
    26461 gttccttgct acaggcggaa tacgtggtgc tggtggtggt ttaagcccct cgctcccctg
    26521 tatttctgga gtattcggct gatagccatt agttattttc ggcggtattg gacgaggacg
    26581 aaacatcgcg ctacctccta cactcagggc aggtgttccc ttccgcaaag taggggaaac
    26641 tgctatcaag ttcatcacac catctgcgat gctcgtagca ccaaaaagcc tcccacggta
    26701 aaccaaaaga cttcatccac cgggacactc gatctggtat atctgctggt ggcagttctt
    26761 ctggcaagaa tttgcctgat aattcccttt ccgcccgtgc cagcattcct tttagactcg
    26821 cattctcttt ttcaagaatc tctatgcgcg cctgtaactc agctttcgtt ggcatggtcc
    26881 gcctcatgct tttcagccac cagcggcaat aaagccctgg ccatcttatg aaccaacaga
    26941 gcatcgataa tgccaagcgt atgccccggc ttaatgttta atgccgcctc aaggtgacac
    27001 ctttccaggt cacttttctc ggcttgttta tgatgatctg gcgtaataac gtcgcccaaa
    27061 acacggctaa ttctttctcg taattgccgg gtgcctgcac acttgatcgc tgtatcgtgg
    27121 agacggttaa ccagttcgcg ataaacatgc ggcttaattc ggatacgttc accggtgacg
    27181 ccctttcctg gcgctggcac cgaactatcc ggaatatccg gatagttgcc agcctcgtaa
    27241 gctacccgca gccagtgcat gaatgtttca gtggacacac aaccgcagtc cacatcgatt
    27301 ttcgcgcgtt gctgttccag ccactgctca aaattcaatc tacacgtatt actttcatgt
    27361 tgctcttttt gtctcaaggc cagcatctgc tgggctattt ccaatacttc atctgccgta
    27421 tatccagcac cgtgaccata catttcgata cgggaaataa tctctgatat acgctcttca
    27481 gttattctgg tcatttcttt ttgcgccatt tcttttcaca ttccttagtc catttttcaa
    27541 tgttcatttt ggcaatatca gtcattccat cacctaagaa atactttctc cggtacgtct
    27601 tgcacttaaa ccacactaca acagccacca gccagaaaat aaaaggccat acggcaatac
    27661 caactccagc cgcgataaag cccaatatcc ataaatgaag ctctccaact tctgtttccg
    27721 gcaatattct taaagaatta aacagcaggc tgaacgaatg gtcgtatgca ttggcagtat
    27781 aagacatgca atccatataa ttaaagtcat agcctgcggc tgccgcccat aatgggcggt
    27841 caagaaaatg ttttagtgtc atcatattaa tttaaggttc aggccagtta tcttcaatag
    27901 caatgcttaa tctttgtagc cattctgcta atttcagcat tgcttctctt tcgcttaaac
    27961 cacacggaaa atcatcaagc gatattgttg gcttgaagtt tcccaaatta tccatttcaa
    28021 cggtcagatt ttgctccaga acggtatttc ttacgcggct attgtgccga agcaaatata
    28081 ctgaacgtga tttattggtt ttgtggtcga acgtatattc ggtaagtatc atctgacttc
    28141 cgccatgatt attaccgcgc cacataatta ctccgtgtta attgaaattt agctattaat
    28201 cttcactttt atcgcgaaca cctttaccgg tttatcaccg aagtgtggat gtgtgattgt
    28261 ttttatttca tatccgttat acgggacgtc aattctgcga ctgaagtctt cgcgcttcgg
    28321 atatcccttt gtgataatca ggcggtcata cttacagtta acgaggcgct tattccagta
    28381 gtcattacac aggcgatact cttccgtttt ctccccgcga atcatggcat cgaagtattc
    28441 acctttaacg gcaagttgca ggttagccat tacctcacct ccagtctcca taccgcctga
    28501 ccaatccggc tggcatgggt atctttggat actgttccgt ctttagccag ctccataaga
    28561 attttgcgca aatctgccga acgccattct tcatcaggaa attccttctc cattgccaac
    28621 cgcagattcc aggttgccat cctgaatgga tattccccgc cgagagcttt atcttgcagg
    28681 gcagcccggg aacgcatcac ctgcaaaacc ttctctttta catccatcat ttcgcctcct
    28741 gcggcggttc tggtagcggc atccagaaca aggcgttccc taaccacgat aaagtgccgt
    28801 cgctcaactc cacgtattcc ccttgtacct gtcctgccat atactcgccg tgctttgaat
    28861 aaattaaaat ccaatcatct tgaggtggca ttcgctcact acagcttatc caaccatccg
    28921 gagttaccgg agagttgcct gccagtctac gcaaaacagc cttaacagcc tcaatacggt
    28981 catcatcgca attttccagc gtatctatgc ggtcgagcat gatgatggcg ttatcaatat
    29041 caggattgcc ggtccactca ttaccgcgat tggattcggc agcctggttg ccagatgctg
    29101 gctgattgtc ggctttaccc agtctgtcgt cgctgcatga atgcccttcc agccaggcca
    29161 atgcttgtcg catgaaatac gcaatatgtt tgccgtggta atcgtcttca tcgatgtgaa
    29221 aagcgatact acgaatgtat tcaattgcgt tttcaatggc ctctaacgct atcggcgctg
    29281 gcggagtggt atatagtttt cgacatttgt atatccaacc ggcatggtca ggcgtgtctg
    29341 taaaacgcaa atcgtcttcg tagcactcac gactacgttc tttccattcc gtccacggaa
    29401 caccgctatt ccaggtgggg cgagtgcagg actgatacag aacaggctct gcttccagcg
    29461 atgccagcac gatacgcgcc agttcttccg cttcttctgc tggcagtaca acgttgctat
    29521 caggtccgta tgtttcgcgc cactgcttga ttgtcagcag tcgctctttg gtaatagtga
    29581 tcatgccgcg tttccttctt tcttattaac aattacaccg tcatatattt cattaaggtg
    29641 ccctctcaac tccatgcgcc ttaatgcaga taacatgtaa tcgcattcaa cctgcttatt
    29701 cccaataaaa ggtttatctt cagggttacc ccaacagcaa ttacccttgg gccacccatg
    29761 tactttccgt actcttccgt taacaacgtg aagtaatccc cagccaggtg gtaaatcctc
    29821 aattgaaata attcccggct cactaataaa gaatcgccag tcgcccattc caagagacgg
    29881 atttttacgg aaacgctttt ttttatctgc caacaagtca gcacgagaac acttcgcctc
    29941 tatcaggcat gatgctgaat ttctgaatcc catagcatct ggctgttctc cggtactggt
    30001 tacagctata aagcggtcat gaaagcaaac cttgaacccg ttgcgcttaa ggaacttgta
    30061 cgcaatctga cagagttctc ggtgtgttaa cgccatctca ctctcctttg atgcgaatgc
    30121 cagcggcgcg ctcggcttca ctttgttccc aaaaccactt gtgaagcgcc ataagctttt
    30181 cgtcaatcgg tgcatatttg cgattaaagt aggcctgagc atctttctca gattcgtccg
    30241 gtaattcgcc agggccaaac agtgtgttat aaatccatgc tagtccgctc ttagcgtcgc
    30301 cagttgcctg ccattcgata atggcagcct gcatgaccag aatgtttttc ccgattaata
    30361 ggtccagttc tttgtaccgg ttgcggatgt atgcattctc gctttgtaat tttgcgttgc
    30421 gcttttctga ggcttcaagt aacgcctgct tatcgcgtag agcttcttcc agttcagcaa
    30481 catggcattc actatcaata aggttgttct ctgctgcttc aagctcaaca cgcagcttcc
    30541 caaccgtaag cgcaatctcc tcgttctcct ggtcgcggcg tttgatgtat tgctggtttc
    30601 tttcccgttc atccagaagc accagcgcaa catttggatt aaaggcagca ataaattcag
    30661 cgtttgcgta agcctgagca tctgtttcaa ccaggcagtt aacatgacat tctgcaatta
    30721 cgccaccggg ttctcctttc catttttgac aaacaaaaac tcctgttata ttctcatgct
    30781 gattgcccga tgtatgccct acgatgtagc atcctttagt tgctttctct gctgcttcac
    30841 gcagcgcctg atagtcaatc tcgctcactg gttgccctcc ttcataaaaa taatccagtg
    30901 ggtcttgtca ccctttcctg ttcgttgacc gataacaggc tttctgtcgg tcagtgccaa
    30961 tatttggcga acaggtattt gcgtttcatt ccatttaaaa atcagaacgc cgtatgtcca
    31021 caacacacga aaggcttctt taaatccctg ccgcaaatca tcacgccagg tatctttatt
    31081 cagccgtcca tatttctttc ccatccaagc gttatcacca acacgctcaa gatgcggagg
    31141 gtcgaataca acaaccggaa acgatgcgtc tgcaaatggt aatgcacgaa aatctgctat
    31201 caggtcaggg ctaattatca gccgtcgtcc atcacacaat gtgtgctctt cctttctgat
    31261 atcgctaaat atcgcccggt cgtccttctt atcgaaccag aacatgcgac tgccacagca
    31321 catgtcgagg attgctgcat gtccagtcac tggttgcctc ctttgcgaat ctgttccgcc
    31381 cattcttcaa gggatttctc cgcatattca ccagacaggc catcaatcgg gtacggttca
    31441 ttagccaact cttctttcgc tgacaaaatc atgcgtgtaa cgtcgaaaac ttcacgcaaa
    31501 gacttattga taaatccgtg attgaacgca gcagcaagac ggctggcggt atagttaatc
    31561 ccctcgttgc gtgcttccgc acgaatttca gccagaaaag catcggtagc tggagtttcg
    31621 ctgtggtgta gggcatcgtt gataatcatt gcagcaacac cagcctgccc tgcatccgtg
    31681 accgacacat gctcaagagt tacggccatt gcgtgtttca gcccggcgtt ctctgccacc
    31741 agcgccgcga gattagtctc aagctctgaa attcggcacg tggcatcaat atttgtgtcc
    31801 tccaagcact taatttcacc aagcagctcc agtgcaacct ttgggttgaa cgcggcaaca
    31861 tgacgagcgt tgttctctgc atttttctgt ccatcaaagc cggtccattt gataacgtct
    31921 tcacatcgtt catcaccggg cgtgtgcacc gcatacgtac cagtacccgg cgaaataaat
    31981 gcgacccatt cgccctgtgt tgcctgtttt gctatctcac gcagtgcccg gtaattaatt
    32041 tcgctcactg gctgcctcct ttgcggagtt gcgcttcgat gcacgaaaaa aaagactccc
    32101 gagtatgact gttaagagct ggtgcgaacg ccgcgttaag aacggcagca tcacagccgt
    32161 catcaatata gagcgcaatt tttttctcca ggcgcgcttt ggcttcctgc aactgcatac
    32221 cccggcacgc acgcgggata taatcagcaa tttgagcgat agctttttcg ttctgtttaa
    32281 acatgcttca cctcgatagg cttgatggtg tctaacagca gtcgacggcg cgtattttct
    32341 gcaaagtggc ggcgtccggt atctttgtgg taaaactcat ttttgccgac gacccacatc
    32401 cgctctgttt ggtgcagttt ttttacctgc ggcccgtctt tggtgatcac ggtgccggta
    32461 tgggttttta cgattgtcat gccactacct cttcgaacag atgaacgaga caggccgatg
    32521 ctcgtccacc tgaaaaactg acgatttgat gcatactcac ggtttattcc tgaatgcgct
    32581 taaactcgat tacccacacc aagggattag cgttccagct ttcttcacca tagatggatt
    32641 cccacaggcg ctggaacgca accttggcca ttgcgaaatc ccccttggga gtaaggaatg
    32701 ttcccgggtg atcaggaagc aaactcccag caggcggaac gccctcagcc cttgcatcgc
    32761 attcgctgat atcgttcaac cgttcaacgc gcacgttggt aatttccaac agaatgcgtg
    32821 atgcccatcg cggcatgtga attgatggac gccacccacc atcaaacttt tcattcacag
    32881 tgtgaggttt ccagtcggca tcatcgggta tcgaccataa gccgtaatca ccaggttttt
    32941 gctcgcaact ggcccgataa atccttgctg cgttcttctc atcgccacga caaaggttgt
    33001 cgttccagta cactgcaacc atcctcattg cctaatatcg ccaacgcttc acggacccat
    33061 atgcgatcac caacgttacc gaacgggcat aaaccatgtt caggtgcatc cataacgtgg
    33121 gtatagacac cattgcgttt ctttgtcggt ttacggataa ccaggcagtt atcggccctg
    33181 acatttttta caggtcggcg agtctgcgtc ttccttcaag gatggcccgg accatctcat
    33241 cgttgaaaat catgccgcgc tctttcactt cgcctttcat gcatccccct tacccatgtg
    33301 cgacgatgcc gccaaaagtg atagagaaca gccagaaata gatcgcggcc ataatgattt
    33361 tgaatgccgt gttcatattt tcagctcctg tgattgattg gatacatgcc gcgccttgcg
    33421 gcatgttttt attttcactt cccctgcctt aaaaatcaag atttattaga gcaattattg
    33481 ttgatgaaga agcgcatttt catactccct gaccatgaat gtaagtacgc cgtgactcct
    33541 gaacacacgc gccacttcaa tcttatcttc cagcgcgaac gcaattttac ttagaccaat
    33601 tttcttcagg agatcaatct ttgctggacc gtcatttctg tcatcggtgg caggacgcat
    33661 agatagcaaa ggctcagccc cgtttgttac gtacttccgc agccaggctc gtgttttatc
    33721 ccttgcgatc tcacagcgcc cggttacaaa ccagaccgtg taaacgttaa ataactggcg
    33781 caccatatca ataaccggag tgatgggagt atcggtgtca caggcgagat taaactcgtt
    33841 ccagtccttt gttaatgcac ctttacctgg tggcggaagc aaatgcagtc tgtcttcagt
    33901 tgcctctgat attgttccat caatatcgac tatgacgata tacggacgtt cctggtgtgc
    33961 gtgtttattg aaaatactca aatgccctcc tcattggacg aaaaaaatgc tggtgggcgc
    34021 actccaccag cattaaaagt gacgctgtaa ctatcagcgg acgtaaatag tgccgccgtt
    34081 ctctttttcc cacgcatcgc tacgtgcata gcaaacatcg agaagtcttc ttgccgctgt
    34141 ttcctctaaa cccaattcga caaccaactg ctcatgacgg cgggtaacca catcaaacag
    34201 ggtatgcaac cctttagttg ccagatcatc aatgaattcc ggttcgaacg gcagctctgc
    34261 atctgccaac ataacctctt gcgcccactc aactcgacgg accaattccg ggcggcggct
    34321 ttccatctct ttacagatca attcatggaa gaactctacc caaccttccg gctggaactc
    34381 gcggaaaatt gccaacggct ggaagtttgg catcaaccat tcgttgattc ggatatcaat
    34441 ggcatagccc atgtcgcagc agaactgata agcaaagtcc agcttagaaa cgatataagg
    34501 acgctcgtta ttgaactctt taggcgatga gatcccataa gccaggaggc gcgggaagaa
    34561 ggagatttgc cctaacgtcg gatgaagttt gcttgcaggg aaacggcgct cagtaatgcc
    34621 atacatttcc ttcttgagcg tcgcaaattt ggcattctca ttaaccagcg cggtaacctc
    34681 tgctttttta ttagcaaatg ccacgcgcgc ttcgcttgca tctttaatag tttttttgag
    34741 ctgttggtta aggtcggcga cctgcttacg cagttcctgt cgctcgcttt tagctttgtt
    34801 atagcgtttc tcaaggttaa aaggatcaag tttcatgatc tctttatatt gagattttag
    34861 cgttgaaatc tgtgagttcc gcagttcaac catcgcggtc atttcattga gttttgtttc
    34921 cagctcaatg cttatacgtt cggcattatc agcacgctgg ttggcgtcat gcgtcgcatc
    34981 gtcgatcgcg tcctgttgct ggcgtttcaa atgttcaatt tgtagctgaa gctcttcaat
    35041 ttctttaccc ttcagaccga gatccaactg catattttca gctgcatcta ccagggagtt
    35101 atggctatca gcttctgcgt tataaacatc aataagctgt gcgtgaagca tctccgctga
    35161 ctgaaccgca ttatcaaaaa aacgcgctgt gaggtcatca caactaacgc ggcgttgcgc
    35221 ggcccggatg ttctggataa tggccgggat accggcattc aggacgtcag ggatagatac
    35281 attttcgatt gattggtttt gtgctgaagt gctcatttca aagttccgta ttagcttgtg
    35341 cttcggtcat ttttcctaag tatgaaggag gaaggactac gcaatttgta tccagtccct
    35401 cacctatggc agcctgtaaa attctggcta aggtgagtct cttgttgcga tacctggtga
    35461 tgacatgcct gataccgccg gtcggcgtaa caaaggcgat cagccagtag tgatatttcc
    35521 gtcggaatgg ccacatagtg caccttgtag attgctctaa taaaaaacgt gatgagtgta
    35581 catcacgttt taaaaatatg gaattattag agcaatatta ttctgattct cgctcaaaaa
    35641 atgagctgat aaggggaagc caatcctctg acacttcgcg aggtcgcggt ttgccgtgga
    35701 aaaagattat tcggcagtct tttggtaatg ccccattccc cctggagtaa cgcgcgctcg
    35761 catattttga accaggttcc acaacatcgg ccttgtaact tacaaaccat cctggataca
    35821 gatcctgaaa tgctggtgta tcatcgccca taaccttttg taagaacccc tggtcacccc
    35881 agcactcagt agtgacacaa cgagaaatcc aaccttccgg atcttgccag aatgaactcc
    35941 agatatgcgc tttaacacta tttggtatcc acagggcacc gctgccacga tattgtggat
    36001 ggtaaaaatc cctaagcatg gtgaagctgg ttggtggatg ctctaggatt gggcgtatat
    36061 caccggcaat aaccgtgtcc aaatccagat agaacagatc atcggttata tccggtcgga
    36121 acaactctat tttcgcccac cagccacggc acttttgcca ctggttgatc aatgggacaa
    36181 ctttgacgcc aggtacatgt aaacgcttca ggtctgtcag gcaaataatt tcatagcctt
    36241 ttggcagttg attaaccagc cactgcacat cggaagcgtt atagtcacca ccagagcgaa
    36301 aaactaaagc aatcttcatg ctgcaccatc acctttcact ttcatcaatg tcaggtttcc
    36361 gcaaaatacg gcaccggtgt cgatatactg ctgattccag aatgtcttcg ggcttttcac
    36421 cggagtgtga ccaaagataa aacgatctgc gcccgaaatt tcgccaccaa tatcatccat
    36481 cgaatcactg atacgctcgc gcgcccagac aacgttgaaa agcggcacct ccttaccgaa
    36541 ttggtattca ttatccggat agtcggcatg ggctataacg atagtttctt gcccggtgtt
    36601 caactcaatg atatagggca gacgctttac cagctccacc agcgcccagg ctaatatttc
    36661 ctgatcagtg tccagcatga agaaccattg tccgccattc attagccagt tattcacgtt
    36721 gccatctgga cttaacgcat caatcatcag ccgctcatgg ttccccatca ctgccctgaa
    36781 ccagggcatc tgcaatagtt ccagacattc gacattttca gtaccgcgat cgataaggtc
    36841 gccgaccgat atcagtaaat cctgcgccgg gtcaaaatcc acacgatgga gttcggacat
    36901 cagtctggtg tagcaaccat gcagatcacc aacaacccag acattcctgt atttggtacc
    36961 gtcgatacgg tgataaattg tgggtgccat catgtattct tcagccattc tttaagagtc
    37021 atctgcggaa tacctcccat tttcccgcat gaaacaacgt caatccgttc acgcgcagac
    37081 tggaataaca aaggcaggtg acttagattt tttggcgtgc cgccggagtg aacgcgtggt
    37141 tcttgtgtag cgtcaacgcc caccagggcg acatgtttga atccgatatg gaaagccagg
    37201 ttcagagcgc catatgcact attgccgctg gcaatttcat tctcatcttc gcaaagtccg
    37261 aaatgtgcgg accagcgcca cgcccaccac tcgggagaat tcgtattttt tggctccatg
    37321 ccacgttcag ccacacgacg gaagcacaga acgccgtctc tgacttcacg ttctttaaca
    37381 tcgggtagtg ccatgcaata acaaacacca cggcgacggc ggccacgacc aacgcgccgc
    37441 atattgtctg gcgatggatc aagtgtgaaa aaataagaag cgcggttcag ccagtcgatg
    37501 gccccattga ccgctataat cggcactccg cgcggcgcaa caaagtttgc ggcgcttggg
    37561 ccactgccga cgataataac gcgatcactg cctctaaatt tattcttggg aaacattgaa
    37621 ttgcactgct cctacttgca ttcaaaatat gtaaatctgc gtgttttttg cgggtatcca
    37681 ggaactgctg ttgccatttt gaaatagaca cctgcgttgg attccgtagg gcttgagggt
    37741 gcgcgccatg ccaatgaagg ccgttttgca gagaacagtc atagccgact aatacaacta
    37801 cttcagcccc tgattcagca gccagactga tagcctgcgc gccgctattt accccttccg
    37861 ccggtccaca atatcgcctg tactccaacg aaaatgattt cgccgccgcc aggttggctg
    37921 tcactttgcg gaatttccct cccggtatgg tggatccgta ttgcttccac catgacaaat
    37981 caccggcgta taaggcataa atgtcatcga acatctgcca ggaattgtta accgcgatga
    38041 ttgaacagcc agttttttct atagcagcac agtcctcacg agtgagtgac ggaccgctac
    38101 cgacacaaaa aacagtccta gtcgccctgg gtggtatgtt cattctcagc tgcaaattca
    38161 gcctccaggc gagcattcat ttcagcgatt accgggtcca ctacagcatc tgcttcctgt
    38221 tcattacgcg gcatgatcga tgccagcgat tcataattag ccttggatga cacgattatt
    38281 ctcccgatgt taatgtgcgc tgtatcaaag atacacatat gcactaatta atttattatt
    38341 tcacgtagcg tacaaccact tgtcaccgtt caatacatgc tcaatagcct cacccttttt
    38401 aaggctcatg tattccagga tggcggttat cgcttgttct gcaccatacg caagaacgac
    38461 gttgtaacct tcctctctaa gcctgcgcat ccaggcgatc tgctctttcg tcggggcttt
    38521 accatttggt tctttaagct caattcgcat gccgtgataa ataccgcatg ctttatcgag
    38581 actcatgtcc ggataacctt ttttctgccc ttcagccttc attttcccgg cggttgcttt
    38641 tgaacgtttc cctccgttag gcgttgcatg caacagctca tagatgtcag ggtgcttgcg
    38701 ttcgaagtaa tcaaaaatga aaacctgctc gaagtgctcg caatttccgt cgcgcaggtc
    38761 tgggttcttt gccagtgctg caagtgcctt cgcatgtgga gaaacttctt ttaccggcgc
    38821 aagcgataag aatggatcct ttttggtttt tggcctggac tgccccttat ttctacgctc
    38881 actaaaagcc tgaaactctt cctcagtaaa gcgcaacata atcagtcaaa tcctgccggt
    38941 cgcatgccat atttacgctg ttttgctgcc tgctcttccc tgtgccattg cgcacactca
    39001 gcgtcacaat agatgcctga ttcaatcggt tcattgcagt aacgacactt acctgtaaat
    39061 acctgactca cgacctgtgc ctgctttctg atgttatcga tggccatgtc tttgagagct
    39121 tctaactgat tcatgctcag ctctgcatca tcaacacgtt ctgccaattt tgtttcctcg
    39181 tgaataacct acttaagggc agaatgatac atttcacaat caaaattgca ctaataattt
    39241 tcttttatta agttaaatat tcaacaaatg actagcagta gaatcaccat catctatttc
    39301 tggcaggctg actatggcta catcaatcac tacaacccaa agcacccggc aatatcctct
    39361 gtcgcggtat gacgaccgca acatagccga tccaatactc agggcagagc tgcgcaaaga
    39421 ggtgatgctt atgtgtgaat cgaacgacaa gaatctgacg atttattacg ttcttcccga
    39481 tgagcaatat cgcccggatt tgctggctta ccgtatgtgg ggcatagcag agctacgctg
    39541 ggttgtgacg ctcgccgccg ggcttgagga tgagtctcag ggtatgactg ttggcaaaaa
    39601 attaaaactc ccacctgcca catggatccg cgaaatgatt cgccatttcc aatatgacgg
    39661 ccaggtgata gggacattat ccattgcgta agggaaatga atgccaactg aatatgctcg
    39721 cgacaacctt ggtcgctatc agactgatgg attaagtgca aaagacttta acaaggtatt
    39781 cgatcttatc cgtaaacagc agcgtcagaa tcggcgaaac gcgcgacgta cactcacccc
    39841 aaggattatg gggatgcgta accgcgaact tgaggcattc ctcagccttg ggaaaaagaa
    39901 agatggcacc tactttacgc ccgaagatat acgcagcttc aacacctcaa ggcaggctca
    39961 taaaacaaaa ttcaagagca cggtacccgg cattacctat gctcagttgg tggcgcagtc
    40021 caccagcatt gatataaaac gcgctaacaa caaagtttct gatggcacag ggatcaaagc
    40081 cgcgacattc ctcgggctaa aacacaacct tgcattgata tctgttaatg cctcagatga
    40141 gtcggtccac cagcatcacc gtgtcagaat tcgatttgag gaatgggata aagccgttga
    40201 ggatattgct gaagacggtg cgaaaaaagc ccgaatcgct gccgatctct gcaagggccg
    40261 ggtatctttc gactgtgatt gtggacgcca tcaatactgg tatcgttata tggccactgc
    40321 tggtaactat gctgtcgcgc cgccaaaaga gtatgccttc cccaagatcc gcaaccctga
    40381 tctgactggt gtagcctgca aacatgtgtt gcacgctatg acgcgttttc agtctcccac
    40441 atggcacaag gccatcatta ttgccctgga aaaagcagct gaacaggtag ccttcggcga
    40501 tgacaagcgg aagacaacaa cctatttcaa aggcgaactg gctaaatcgc tcgcgcgcaa
    40561 ccggacaaca acgacggatc aggctaaagc tgcgcgtgaa tatgagctgt atctgaaatc
    40621 tcaggatgca ttaggcaaaa aactacgcgc caaagatagc gccacggaca acgttcgccg
    40681 gttgttaaaa aaagctcgca ccacggcaaa caggaagaat gccgaactaa aagcatcgcg
    40741 ggtgagggaa gcccaggctc gcgctgaagc cgacgctctc aaaaaagccc tgcaaacgca
    40801 ggcgaacaac ctcataaagt ttttcatgag tcagggaatg gacaaggccg ctgccaccgc
    40861 gcaggcgcga agcattcttg agacacaaat taatgaagcc cgtaaacgga aaggataatc
    40921 gatggctggt ttctttgatg acatgtttga ggacacagaa ccatcacaac aagtgactgg
    40981 tgataacctc ccggacaccg aatcggatcc ggatattcca ggcgaaggtt ctgaactgat
    41041 tgaagaggaa gatattgatg ctgaaatcga aaccgatggt gttaacgttg gtaatattgt
    41101 tgatcctgtg gaggacaatc accttcccaa tctggatcac ggcctgctta gtgattctgg
    41161 tgtgcgccac cgttatcaag gtcatgcagt ttttaataac cttgtgcgga tggactggct
    41221 caaagcaatc aagctagacc ctgactcatt cgatgcagtt ctgtatcgcg caatacctta
    41281 cataaacaaa aatgcacctg aaacggcacc tgaaataata gaaccgaacc aacgcatata
    41341 tgactatcag gatccagaac tgataacggc cctcgactgc ccggatgaga tggacgcctt
    41401 ctacgcgcta tacgacggca gtgataatac gggaattagc gacagtgctt taatccttcg
    41461 gttagccgcc gtcaatgtgc cagtgggttc tatgctcgaa tggctggaac agctgtcaga
    41521 cggcacaacc attcgccgct tctggtacat ccataaaata ttcaattacg gcactgccag
    41581 ggtaggcagt ttgttttatt gcgtgccttc acgcgccttt gaagggaatt tcatcggtga
    41641 ttctgaataa tcaggaatgg ctactggcca tctttaagaa aaaaggtctt actccaaccg
    41701 gtaagctgga atttgccact attgatggca ttgattcggc gctcgcacag gctttaaacg
    41761 aagcgttcga ctcacaagtt gtcagcttta atgatcgcac taaccagtca ttcagggagt
    41821 tcctgaaacg caccccaaga gatcgcataa cgctcggcac ttttagtgat gtgaaggagt
    41881 ggttgtcgtc atttgaagcc gatcgcgccg ggcgtaaaga tactgcctct gctggcccgg
    41941 taaataagct ggcaatgccg cttgtgaatc tgtctcgttc tcccgcgttt tcaatttatg
    42001 aaggtgaact gtgccgggat aattacgatg aagggcatgt caccaatgaa aatgatgaga
    42061 ttgaagccct ggtatcgact atccctttct cactggaata ttcgctatgg atagccagtg
    42121 acgagaagga atctcttggg atggttacaa ctgcattagc attctggcta cgaatgtatg
    42181 ccagcctcgg gcaggcatct ttcactcaca ttgccaatgt cggcggttat gagataccgg
    42241 ttacctgtta catagaaggg caaaaatcaa tcgcatttca ggatctgacc accggcaccg
    42301 ccgacaacag gctgttcgcg gttggattga acctcaccgt tgtggcggag cttcctatcc
    42361 tggcttatat gcagcaaacc accggcacca taacggtaaa agcgaaaatt ctggaggaat
    42421 gagatggcca caaagaccac cacagccccg gaaactgatt caaaacgcac tcagctattc
    42481 ctgcaatctg tttcaattgg gcagaacgaa atccctcgcg aaatgatcgt aggatgtacc
    42541 tatgtcgaac ctggggagct atctggtccc cagcttatgc tcatggtcag ggattcaacg
    42601 gcttacgtgg tcaataagct gggggtgaaa tttgggacaa tactgacagt ttcacttggt
    42661 gatccggaag gtcatggcgg catcctcttc tcggaagagt tctttgttct taaagcgccg
    42721 cgcaaggacg atactgtact gatttacgcg tttagtaacc cggtgcggtt attaaaagtt
    42781 ccgtccacca gcgcacagta ttttgttgat aagcccccat cagccgtagt ttcctctctt
    42841 gcccctggtc tgaaggtaaa tgctgactca ttcagaaaaa catccacata ccacctaaat
    42901 gttggagaaa aaccgaccaa ggtattgcag gagatagccc gcgataccgg ttctatgtgc
    42961 tgggcatcca gggggacgat caattttaaa agtatggaaa aaatggcaaa cgccgctcca
    43021 tcgcttactt atgagtccgc caatcccaac acatccggat ttacaattag tcagttcaac
    43081 atcctgaatg ccgattatga ataccagcgc cgccacaatt acagaatggc cagttatgac
    43141 atgaccaaag gtgtggttta ctcaggtaac caggaagacc ccattaaatt tacgagcaat
    43201 cccgatccta ccgcgctggc gaactacaac aaattcattc tcccccgcct cgatatgctg
    43261 gtggaaggaa atgccgcgct aactccgggt acgacgctga aaattgtcgt gcataacacg
    43321 gcaggtgacg gagaactcga tgaatctatc cctgacaaaa tgatagtgat gtccgtgact
    43381 catttcgaag accgcttccg ttttgtcagc cgagcacagt taggagtggt gaatgggtag
    43441 tttgacaggg aagtatcggg ctgtagtggt aagcgtcgat gaccctaaag gtctgatgcg
    43501 tacacaaata cgcgttgtcg gcatgatgga tgggttacca gatgcctcat tgccgtgggc
    43561 agaagctata ttgtccaatg caaacacgtt ttcaccattt ctgcccggcg ataaagtatg
    43621 ggtagaattt ccctacaatg gggattctcg atggccattg ataatcggtt atgcacagga
    43681 tgcatccggt ggcgctccca atgtgccgcc tgaagcgtca ggacaaggtg aaggctatgt
    43741 accgcctgaa gtcgaaggtg caccagcaca accatcaacc agcgccaaaa aagactttat
    43801 ttcgtcgcgg aacggactaa tggaggtccg gacggcgggc ggagcctggg ccgttacgca
    43861 cttgaaaagt ggaacaacaa tagggttcaa cgaggccggg gagttatatg ccatttctca
    43921 aggtccggca ttcatctctt ccgcaggaaa tctcgatata aagtcaggcg cggatgtcgc
    43981 cctgaaggcg gggggaagta tggcgataga ggccagcggg aatctatcca taaaagccgc
    44041 tcaagtctct gttgacaagg cttaagaaaa gcccggcgtt cgggcttttc tgttatgacg
    44101 ggttcaattt tttatccgtt accgcgcgac ggtttctgcg tgataaacgt ctcaagcatc
    44161 ttttccgcaa ttgccgacca ggtgtgacac tggacctttt cagcattttt cacgcgatca
    44221 acgcgagcaa taacctcatc ccaatcaatc cgcgacttga taaccatatg gttcaccaaa
    44281 gccaggcgat ctggcggaag gcaatcggga ggcgttaata ccaacgcccc gcacattgcc
    44341 gcctcaagta cagttaatcc aaggctttcg ggatgcgtaa cgataaacac gtcactctta
    44401 cgcaattcag ctgcaaattc ggttgctggt accggcgtcc gtctgtatgg ggttaccgat
    44461 atattccccg gatcaatggt aatcaatccg tcatcggtca acgttctggc ctcatacgga
    44521 acggtcagac gctgaaggtt cataaggata cttaaggagt gatcaaaccc actaacatca
    44581 aatgcagcgt ggtctacaaa aatacgcaga acatcgtctg ttttggtttc cagatggaac
    44641 agatcctgat tcgctgccca tccaacatgt ttgttaaagc gattatgacg ctctaacctg
    44701 ccgggattat ccaggtaccg ccaggtatca tcgcggacag taaaagtaat atcgactggt
    44761 gccgaatcca gcatagaacc gtcgtatacc tgggctaccc atccagtgaa tcgacgacgc
    44821 agttgcacgc ctatttccct gggcaccgta gtaaaatacc gcaatcctgg cgctaaaatg
    44881 gctttcgcag aacacgaggt cgcagcggtc aacacagctt caacataatc ctccgggctt
    44941 tcgacgccgg gggaatatgg acgatggaat tgcaatgtta cccctgcctc actaaaggcg
    45001 caggccaggt tgtaagccca catttccgta tatgttttca catcactgat agcttcaaat
    45061 tttcgcccaa tgatcaggat gttcatcggc ttttcctcat tccattgcat taataatcct
    45121 cttgccagtc agcaccagca tagttatcaa accgtgagta ttggccgtta aaagccaatc
    45181 tcaccgtgcc aattgggcca tttcgttgct ttccgataat tacctcggca atgcccttca
    45241 tttcgctatc cgggtgataa acttcgtcgc gatacagaaa catgatcagg tctgcgtcct
    45301 gctcaattgc tcctgattca cgtaaatctg aatttaccgg tcgtttgtcc gcacgctgtt
    45361 caagcgatcg attaagttgt gacaatgcca ccaccggtac ttgtaattcc ttcgccaaag
    45421 ccttcagtga gcgagaaatc tcggcaattt ccagcgttcg gttatcttgc agctcgggga
    45481 cgcgcataag ttgcaggtag tcgatcataa tcatgctcaa accaccattt tctttataaa
    45541 cacgacgagc gcgggaacga agctctgtag gtgttagggc gcttgagtca tcaataaaaa
    45601 tattctgctt gtccaacaga atccccattg cgccagaaac ccgcgcccaa tcctcgtcgt
    45661 taagttgccc tgttcgaata cgagtctgat caacgcgtgc aagagaagcc agtgagcgca
    45721 tcatcagttg gtggctcggc atctcaaggc taaaaaccaa tacgggctta tcgttacgga
    45781 ctgcggcatt ttcgacgaga ttcatcgcaa acgtagtctt ccccatcgat gggcgggcgg
    45841 cgacaataat gagatcggac gcctgaagtc ctgccgtctt cttattgaga tcggtaaatc
    45901 cggtatcaag ccccgttaca ccatcatgcg gtcgctgaaa caactcttct atgcgagata
    45961 ccgttgcatc gagaatgctg gcgatatctt ttggaccact accgctcttt tgtcgttttt
    46021 cagctatttc aaaaacgcgg cgctcggcca tatccagcaa ttcattgctg cccctgccat
    46081 cctgcgcata tccagcttca gctatttcat ttgcgacgga aatcatttca cgaacaaccg
    46141 cgcgttcacg aacgatatcc gcataagcac aaatatttgc cgcgctgggc gtgttctttg
    46201 acatctccgc aaggtacgca aaaccaccgg cgcgttctaa tttaccgttc tgttcaagtg
    46261 cttcagcaag tgttatcaaa tcaatcggtt tgccatgact taataacctc tccatctcac
    46321 tgaaaatttc acgatgagca ctggtataaa aatcatcagc aactatacga tctgcaactt
    46381 catcccagcg gcagttatca agcattaagc caccaagtac agcttgttct gcactaaggg
    46441 aatttggcat ggattcaaga ggggatgcag acattagcac tccacccagg cgtgctgaat
    46501 gtcagatata atcggcatac tcaaatcact cctaacgata tgagtcatca ccagaaaatc
    46561 aggattaatg cgccggactc ttcccggctg tcacaccgaa tcgccaggat ggtgaatccg
    46621 cagtccgacg ctatgaacgg ggcttgcaca ttccggctac ctggtttgtt gcctgagcta
    46681 ggggaaaggt taccccttta acgtcaccag accgctaacg acgcatgtgc cagacgccgt
    46741 gttacaacca aatatggtgg cccctaccgg acttgaaccg gtgaccgtgc gattatgagt
    46801 cgccagctct aaccactgag ctaaagggcc ggattactgc caattttgct tacgctttta
    46861 tttcaccgga acaaacggaa cagcggtatt actggtcata tactgcggta atttggcaaa
    46921 agccaaacat acagaaccag tgacacaatc aaacagagga cggcataaat atataaccgc
    46981 acccatagcg ttcgacattt gttcggattg ttctgcatcc tctcactcca ttatttaacg
    47041 aataaaaaag ctgcggtgcc gggttcctcc cggtgtcctt tggctggtta tccaccgtgg
    47101 acgtggaaac aaggagaaat aatggacaga tataaccatt tccccgcgtg cgcttagccg
    47161 cattcaccgc aacggaaaga gcattcttgg tggacctgta gattgggata tgaacccgtt
    47221 acaggagaat gctcttacct gttacgtgct ccgtttcgtg gagctaacgg cgggtgatca
    47281 gaccgcacca gactggactt atttcagcgt tatgctcatg ccagagaatc aaactgtgat
    47341 ggtcggtgct gaactccgac acagggttgt agcaagcccc gcaaagcgcg cactactgta
    47401 gttgcggcac atcagcctgt gcattcacca caatgttgag aacactggtt gtcacgcggc
    47461 aacgcaacat ttattcgtag attgggatat gaccccgtta cgccagtgtt ctcaacgttg
    47521 tagttgttat gcgtattctc ttactaaccg cacaccaata catttcccat cacgttcatt
    47581 tgtctcgaaa cgtgaatact taagttcgcc gttaataatc gctgttacaa cccgtatccc
    47641 gttaaacgcc cacatatttc gctccattta cacacattac cagtcgccgg ttacggttcc
    47701 ggccaggcct cttcctcaac ggggtgttct ccatacggac taccgtttat tggtcgttcc
    47761 tgcggtttat gttgtgaagc cagatgctta tcttctggtt gcttctaaga tctgcacttc
    47821 atcacaacgg taagagcact cgatgcattt aagccaggcc ccataaggga gaatgctctt
    47881 acctgttatg ttggtgccga ttaacgggat cgaaccgctg accattcgct tacaaagcga
    47941 ctgctctacc aactgagcta aatcggcaat gtggtgggga gtgatggagt cgaaccaccc
    48001 gagtcgcaat gacagtagat ttacagtcta ccccgctacc cctacggact aactccccta
    48061 aagtggcgat ggtgggtgga ttcgaactac cgaccagttg gttaacagcc aactgctcta
    48121 ccgctgagct acaccatcac ttgccgggta cgtctccggc gagggcttcc acctccgtat
    48181 gcttttcggc gcaccgcgcc ctggctgcaa ttcggtaaca ggggatgcat aaccctggct
    48241 tccagcgtga ttagcgcctt cagcatgacg ggatataccc gtaaattcgt ggaactgtac
    48301 ccaaagtgct gttaagcacc gctgttacgc tgaaaagaaa acgcaacagg aaaggacgct
    48361 gaccaacaga tggccccttc tcgttcatct ggttaatcac accagcgccc ttacctgttg
    48421 tgcctccccg ttccctaata cacagacggg gacactctgc ggtcgatttt ttgacggggg
    48481 acgactcata ccccatggca tctggcttct taggccgcta ccatcatcag atcatcgttt
    48541 gcatttactt taatggtcag tttctaaacc gccgcaaagt cgctaaccat gacgaaaacc
    48601 ctgaaaaaaa cgcccacccg aagatgggca aactggacgc tcgtaacgca cttcggcgtt
    48661 gccacttagg cgcatggtca acctggcaac tcggtggttt gtctgggagg actaggccca
    48721 gccatgctta ccgccgcgcc tgtcgcggct aacagctaaa tcgctctata aatcacgatt
    48781 cattgaggta atattacact aataaattta ttagagcaat atacccaaaa cgtcatgagc
    48841 tacacctcga gtgtccccct tacaagacac agaacgtctg gcaaaaagag gttccactct
    48901 gaagccactg tcatgataaa gctctctgat gtttggcgcg ccactgttag taatgagaac
    48961 ctttgcacct cgacgatgag catccgtcaa cagagacacc aggcgttttt gctcttcaaa
    49021 cttaaagtca tgaccggaat agttcgtgaa tccctctgta tttggaagcg gttcatacgg
    49081 cggatcgcaa aagatgacat ctccttctcc ggcagcttca atcaccgctg caaaatcacc
    49141 gcatacaaac tcagaccgcc cttccgcacc gaggaaggct tccatctcct gtaatgggaa
    49201 atacggagtt ttatacttcc cataaccgac attgaactca ccggcctggt tgtaacgcgt
    49261 caatccgtta aaacaatgtc ggttcaggaa caaaaacgcc gctgcgcgat gtaaatcatc
    49321 atagacttgt ttgttaaacg cattccgtac tgccaggtat ccttcctgtg tgttgtagtc
    49381 ctggaagaaa cgatgtgcca gagtgataag tgaatgcgcc tcgcgttgca gagtcttgta
    49441 aaagttaatc aggtcagcat tcacatcatt tagcagattt tcctggtatc cggcattcat
    49501 gaagacagct ccgccaccaa cgaaaggttc aatcaggcgc ttcccttctg gcaaatagcg
    49561 aaagatttgt tccagaacac caaattttcc accagcccat ttgaatatgg accgttcgaa
    49621 ttctgccgct ggtttaactt ttcgctcttt tgtttcactt ccttctttct gccgacatac
    49681 ggccttagta atccgatcgc caatccagcg cattactggt attgccatac tattgccgat
    49741 cgctttgtaa cgcggtccgt cagctgcaag cattgcggcc tcttcttcgc ttaaatctgg
    49801 atagtgattg tgcagatatg ccagttcatc tgaattaact tttttacgct tttccgtagg
    49861 gatcaacgta tgccaatcag gaaaaccttg cagcctttca cattcgacag gggtaagacg
    49921 gcgcattcta ccgtcgccga gcaatacaat tggtgcttca tggttacatg tcaaagttgg
    49981 tgccgaatta tcggttttta tctcagcccc tccttgccca tgtgccatgg caattatgtt
    50041 tgtatcatcg caagttctga tagcgatgtt tgaagtatat ctggtagttt ccttcccctc
    50101 gcctctgctc ggcgcaatat tccggcgcac gccttcgaac tcaaaaagta ccgttgcggg
    50161 atcgagatct gttcgagcac ttgcgacaac aaacacgcgt cggcgtcgtt gtgccactcc
    50221 gaagtattgg gcatcaagga ttctccaggc cacttttcgc tgcggtccat aaatacaacc
    50281 acactgcggc cactttggaa catggcaacc ggttttgcca tcccaccgcc agaacgcgtt
    50341 gctttttcct gattcaggtc gatcacctgg ttcaaatggc acatcttctc cagccaatcc
    50401 ggcaaggaaa catccgaagg cgttatctgc cgatgacaag actcctggga cattttccca
    50461 gacgataacg gctggtttga gaaatgactc agcccgtttg tcgtcaattg catttgcaag
    50521 ctccacatac tttaaagtta gcgcgccacg ctcatcatca agcccaccac gtaatcccgc
    50581 gatactgaat gcctgacagt tatggaccac cgcaccattg aggatatagg aatgatcacc
    50641 ttcgacttct atgttgtata cagtatctag gcctaccgat ttaaactctt tgactgttcg
    50701 taaaagcatt ccatgagcca atcttgattt acgtgacaat ttctgcgggc agattgttac
    50761 ctgataataa ttccgttgat ttaccacgcg atcctcgatc acttttttgg gctcaacttc
    50821 aataaagctg accgaagaaa cataaccaca agtctgtgac aaccccgcaa cgccccaagc
    50881 aagcgcagaa ctaacactat taattctaaa tcccgcttta ccacttggtg tcccatcagt
    50941 atcaagatag ccttgtaaaa acacatgacg caatggatgc gacatcaccc atgcagggat
    51001 acgcttagca tggcttaact cgccaaaatg ttcattaagc caattggcat aacacgtatc
    51061 attcaaggtt actttgatgc tccctcggat ttcgcttgcc acggaaaata tgttttctgg
    51121 tatgcggcaa tgaaactttc tcaatttctg gcaatttatg ccaaaaacaa ccgccttctt
    51181 agatttacct ctccatctcc taatatatcc atcgccaaca taagcgcccg caagatacat
    51241 agcctgttct tcagacaaga accgagaaca aatatctgga aatgcaatat tgaaattagt
    51301 tagagcgcac cattgatatc ccggcatatc acatgctgct cgccattccg gttcagacaa
    51361 caactctctc ttaaaatatg tgccattttt ccgggtgttt tgggctttcc accgaacagc
    51421 caggaaggga tggtcattgg ttgttcttat acctaaaggc tgcccaacgg cattaagtaa
    51481 ccccgtatta gctatttttg aaccaactct ttttacttgt tgtaaccgcc cgagatgact
    51541 gactacgtaa tcaccagggc aaacattttc tattggttta tagccatttt tacaaagaac
    51601 catatgcccc gcggtaaaac atggtgttcc cccgacgagc acgtcagggg attcgatttc
    51661 cccagccaag acttttttgg caagtttttt catgtcgcca aggttggcaa catggggcca
    51721 gcggagcgca agaacggccg atggaaaagg ctcgatttca gcaaaccacg ccggacgcat
    51781 acccaacggt tcccaggcaa tactcgcggc ttcaattcca ctgcaaacag atccatagca
    51841 cagctctttc actgcttagc ctctccgcca agggcattta ccagagcatc aaccaggcac
    51901 gaaatttcac tggtcaacag gaagaaatct gcgtccagtc gctgcgcaac atcttcacta
    51961 tcaatatcag agttctgctc aagcaattca tccgcaaatt tgacgctggt aaggctgaag
    52021 ttatggtcca gtgtaaattt aatgcggttc tgccagtcga gtgccaactt agtgacgagc
    52081 ttgccagctt ccaggtgtgt ggaaatttca tcgcttccca aatcctgctt tttcactcgg
    52141 gcaataccgc catcctcaag cactgcctta agttctgccg catcccccat ttgaaatccc
    52201 tgtggagcac taccatcacg tacccagtcg gtcagcgtta attcaatggg attttcaaca
    52261 ctcaggggaa cagcaggaag agaacccaga gacttacgca taagcgcgag catatcctct
    52321 gcctgccgcg cgctggcatt gatatagata cgtttagttg aaccgtcgta gatcgcctgg
    52381 ataacagaaa actttgaaaa agcccgtggc agaagagaat gcagaacttc gtctttcagg
    52441 gagtccttct ctgttttctt cagtttacgc gcttgttctt gctcaagttt ttcaattttt
    52501 tcttgaatag ctcgctggat aaccggcggg ggaagaattt ttgtttcgcg ctttgcttca
    52561 acaaggataa aaccatttcc atgcatagcg ataacttcag aattatcacc aaatggcgat
    52621 acaaaaccga acttggccat atcctgacta ccgcatggcg tgaaaaggat cattttcttt
    52681 ttatcttcta agtcggtcag atccgcctca cgagaaagtt tataaatagt aatgtttttc
    52741 cagtgcttaa acatgttgta acccttgaat atcaaccaca gaaagctcgt ctttgtagaa
    52801 aaaggccagg ttgtggcacc ccctcgtttg agcgtatgag ctgggaccaa tttcgttctt
    52861 ccagacaaat ggcttcaaat ccgtacggcg aagcataaaa acgcgattta ttccgctctg
    52921 attcccaatg aggcaaaagc cttctttcac cttgatagcc tgcaagttgt cgagttcacc
    52981 gctggttaca cggctatcga actctttgcg gcttattagc tccatctgca tctgacgact
    53041 ccaaacaaat gcccattgaa gggcgatggc tgaatggtac cgaaaatacg acataaaaaa
    53101 caatatttat tagagcaatt ttgcgataag taaacgccat acagaccaca aataacctaa
    53161 gttaaaataa cgaaaatcag agcaaataat tggtgatggc gtggcaagca ttgcaacaaa
    53221 agacagcatt tgttcggggc acggaggatt cccatccagg cctcctgtag agagtgaacc
    53281 actacttaaa gtcaacggag tcgaagtgtt agttgatggt aagcaatatg cacagcatac
    53341 cgatggaaac agtacgcacg gtgggcaagc tatatcaacc agggcatggt ttaccgtcaa
    53401 tggtaaaggg atcgtatgcg ttggtgaccc tgtttcatgc ggatctaccg tagcgtccgg
    53461 agacggcctg gttcaggtaa gttaggagat atcatgctgg aaaaagacta ccagttatcc
    53521 gcatataaaa aattggccgc cgccggtggg atgaaaacac ctggtgccat aacatcggca
    53581 cgaaacagtg ctaacacagc aaaactgctt gcagaagaat tgaccggatt aattctggat
    53641 acaattgtct atcccgacac tattaccagc tatgtttcaa cgatcagaac aaccacaacc
    53701 ggcttaacga acattggaga actggcaact aagcacgcgg acctgttggc tggttatgca
    53761 gatctgtcaa tgctgcttca actcgatatt ggttgggatg tttactgccg tgctaatgag
    53821 cgagaagttt cagaactgcc gatctctatt gccattggtg atgtgaatat tactaaatcg
    53881 cttgaggacg ctgttaacgc gcttaataca tcaagtttag tcgctgcaat gggggagatt
    53941 aaccaggccc ttaacactgg ctcagaaagc tcgtcaggct ctggttcagg cggcggcact
    54001 gccactcccc caccagcact aacagaagag caaattgaat ctctgaaagt agcaactgaa
    54061 cagtttgggg ttgttttcaa ccagacaaca gcacccacaa ctgcgttaca acagcagtat
    54121 gaacgagcga atgaaagcgc caacgtagcc ataactgctt ataaccatgc tatcggtacc
    54181 gcgcttgcgg aagcatcagc aaataaggcc agcacagcca gcgcagtcgc cgctttggtt
    54241 cctgattctg ttcttgatga attaaacaaa gcggcacggt aacaaaggac ttcattgata
    54301 atttttcttc aggaggaaga catgtcattc ttttctacgt taaaaacagc tttgtctttg
    54361 aaggagaaac ttgctgctac tggtgttctt gttctgattt gcgcacttgt tggtgctggg
    54421 tttgcatggg aacgtcatca gctaaagcaa gccatggaga aaattggcag tcttgatcag
    54481 gctgttaagg aacgtgataa gtcaataatg gatcttaacc agaccattga gacgatgaac
    54541 aaagcagagc aacattttca cagccaggaa gtgaaaaatg aatcagaaca agccaaatat
    54601 gctgacaggc aaatggaacg aaaagctgaa gttcagaaac aactggttgc ggcgggtaat
    54661 gttcgccagc gcattcctgc tgacactcag cggttgctcc gggagtcgat cagcgaattt
    54721 aacgccgacg ccgacaaagg ttaaccaccc tgcccccaaa agtgcgttta tgtgcaggat
    54781 gccagagttt agcagtgaat attttgatga tctgccagcg tatatcctcg atacagaaac
    54841 gatgctgatg gggattaaca ggaagaatcg caacgttaat gattacaacc gcgctatcag
    54901 cggtaactaa aagggatttt tatgtctgat aaagtaacag taaagcaaac tatcaacaaa
    54961 gcgacttcaa tttacaaaat tgagcacatc actgttggca agccaggatc tgaacaatac
    55021 cgtcatgctt tctagcttgc cgatcagctt ggtttaaaac acccggattg catcgagcat
    55081 gtatttccga cctatgctga tgagcaatgt actcatgttc ttaccgaaga ggattttttc
    55141 agcactgaag aacgagaagg cgttgatcgc tgcattggtg tgatttgctc ttcagtgagt
    55201 tatgagttat tccctaatgt ccatgaaaat ggtggtattg gataccaatt cctgtacgaa
    55261 ggcgatgagc ttaaatgtta tgaacatggt cttcttatcg aaagcgtaga ataataccct
    55321 tccttccaac cggctatgtt ggccggttta ttcaacttat ccacagcata gatccaataa
    55381 acagatccta aagagaaccc aagaagatcc aaagaagatc ccggatcgct gtaagccgcg
    55441 ccatttctgg cctgaaatgg gatcaacatt gactatacgc gattttatgt tgactgtgca
    55501 cgatttattg ttgactgcac gcgatttatt gttgactata tgcgacagaa acattgactg
    55561 tacgcgattt tagagcctga ctattcacag ttgttgataa ctgcaatcca gatgacgcca
    55621 ggccgcgcca catatggaga aaccacgatg ctggaagaaa ataaaggctt ccttagcgtt
    55681 gaagaagttg caggaaatac aggagaaatc cacagcctga aacccaataa caatagcact
    55741 atacaaccca ttgctttgtt gcgcttaggt gtgtttgtgc caaccttaaa atctaccaat
    55801 gtggcactac gtcgcggatc gtcagttact acaaacacaa cgaacgcaac cgaagaacta
    55861 tcaagcctca aaattgttga gcaggaaggc tatgagggaa ttgaaattca tggtccacgc
    55921 ctggatatgg atactgattt taaggtgtgg gtgggcataa cctccgcgtt gtttgactac
    55981 gcacctgatg atgacggcat aatcaccctg ccattctccg agtttgccga tcgatgcggc
    56041 tatccacgta agcgcctttc aaaggcgttc cgtaaaagta ttgatgactc tctgacacgc
    56101 attcagcaga cagttgtcaa attccgcttc ccggcggcaa aaggtcatct caataacatt
    56161 aacgtcaact tgttggcata tagcagcctg aataccgagc ttgatgttat cgagatccag
    56221 ccgcagaaac agctatctga actttactat gttgactata agcgaatcct gaagctgaag
    56281 atgctggata agctcgggcg caaagagacg gccaaggtac tgtatacatt ctttgaggct
    56341 ctacccgcca acccggcacc tgtcagcatt gagcgcctta gagcaaggct taatctcaaa
    56401 tcatccgtta gcgtgcaaaa tagcgttatc agaaaagcca tgaaagattt ggaagctatt
    56461 gaatatctta aattttcaga gataaaaaac ggcaggaaaa tcggctttca gatccataag
    56521 cgcaatccat aatattgact atatgcgata gcgagaagtt gactataggc gacattcgtt
    56581 gacgctggtg gatttttgct ggcgtcaata ttctgcaagt cgctattgag atggctttta
    56641 gggtcatttc atcgcgtata gtcaacgttt ctcccgacaa tatcttacat agtcgatctt
    56701 tggtggagtt aaatcgacta cagtcaactt ttgactgtag tcacatcgcg catagtcaac
    56761 tattcacatt aactttcgcg catagtcaac atttgcgcgg ttctcatcaa gcagtggtat
    56821 tgatatgcaa gaagagaaac aacactacct ctacgttctg gtgccggaga acggagatac
    56881 ttttaaaatc ggcatttcat gtggtccatt ggcacggttt aaagggctac aagtgagtcc
    56941 cgattttgcg ctttcacggg tctatcgtgg tacgcgtttg gcaatggtta atcttgagcg
    57001 ggctttacac gcaacctttt tcccctggaa tgcgccgtgg gagaaaagcg ccggtggcgg
    57061 gcatactgaa tggtttacac gagagtgtct tgataaggtt ttggctcata tcgaatacct
    57121 aaatgatatg tggggaggga ttctcgagcg cattaagtag aatgatttac ttcagcgtcc
    57181 agtagatgct gctcgctctt tcgaaaaaga actggatgtt acttctatcg tgactttcaa
    57241 agatgacgca ggaatgaggg acgtggctta tgtctccata tctggctatg aaccggacgc
    57301 gatccgcgct caatgtgaat tgctgaaagc aatgtttatg cttcggacta aatatccctg
    57361 ggagacaggg cgggtgtgct tccctatgga agagttaacc gccaccattg attctcagct
    57421 ttaccacgat aatccagaga agttttttag ccttttagcc ggtaatgggc ttaactgtgt
    57481 gtccgggctg gggcgaagta gaatccaaca tgcctcgctc ttcggtccct tcttttacga
    57541 tcgacacggg tactttgagg ctgaacttcc ggcgcttacg cgtgctatag atactatcga
    57601 tttcgaacga ttattcgctg ctcttagcaa ataacactga tgcccctgaa cggggctttt
    57661 ttgtgccttt cttgtaactc tcaatcgtgc aaaatgaaca aaacatgcag agaatgccat
    57721 gtacaagcat ctacgcatac attattattt tatgcagcat ttttaattaa attcaaaaat
    57781 acagcataaa ggatgacttt cgatgagtga ttccagccag cttcacaagg ttgctcaaag
    57841 agcaaacaga atgctcaatg ttctgactga acaagtacag ttgcaaaagg atgagctaca
    57901 cgcgaacgag ttttaccagg tctatgcgaa agcggcactg gcaaaattgc ctctactgac
    57961 tcgagcgaac gttgactatg ccgtaagtga aatggaagaa aagggttatg ttttcgataa
    58021 acgccctgct ggctcttcaa tgaaatatgc gatgtcaatt cagaacatca ttgacatata
    58081 tgaacatcgc ggagtgccaa aataccggga tcgctacagc gaagcgtatg tgattttcat
    58141 ctccaatctt aaaggcggtg tgtcaaaaac tgtatcgacg gtttctctgg cgcatgcaat
    58201 gcgtgctcac cctcatcttc tgatggaaga tttaaggatt ctggttattg accttgatcc
    58261 gcaatcttca gcaacgatgt ttttaagcca taaacactct attggtatcg taaacgcaac
    58321 atctgcacag gctatgttgc agaatgtaag ccgtgaagag ctgttagagg agtttattgt
    58381 tccttctgtt gtacctgggg ttgacgttat gcctgcgtcg attgacgatg cctttattgc
    58441 atccgattgg agagagctgt gcaatgagca tctaccgggt cagaacatcc atgctgtcct
    58501 gaaagaaaat gtgattgata agctgaagag cgattatgac tttatcctcg ttgatagtgg
    58561 tcctcacctt gacgccttcc tgaaaaatgc tttggcctcg gccaatatac tgtttacacc
    58621 tctgccgcca gcaactgtcg atttccactc atcgcttaaa tacgttgccc gccttcctga
    58681 gttggtgaaa ctcatttcgg atgaaggctg cgagtgccag cttgcgacta acattggttt
    58741 tatgtccaag ttgagtaaca aggcagatca taagtattgc catagcctgg ctaaagaagt
    58801 gttcggtggg gatatgctcg atgtcgtcct ccctcgcctt gacggttttg aacgttgcgg
    58861 cgagtctttt gacactgtta tttcagctaa cccggcaacg tatgttggta gtgctgatgc
    58921 attgaagaac gcgcgaattg ccgcggaaga ttttgctaaa gcagtttttg accgtattga
    58981 atttatcaga tctaactgag gagtaagaaa cccccatgtc aaagaaaaac agaccaacaa
    59041 ttgggcgaac ccttaatcct tcaatattaa gcggatttga tagttcttca gcctctggcg
    59101 atcgagtcga gcaggtattc aagttatcaa ctggtcgcca ggccacattt attgaagagg
    59161 taatacctcc gaaccaggta gaaagcgata cctttgttga tcagcataac aacgggcgtg
    59221 accaggcatc tcttacgcca aaatcattaa aaagtatccg aagcactatt aagcatcagc
    59281 aattttaccc tgcaataggt gttagacggg gtacagggaa aattgaaatt ttggatggtt
    59341 cccggcgtcg agcttctgcc atcttagaga acgtagggtt gcgggtttta gtcacggacc
    59401 aggagatcag cgttcaggaa gcgcaaaatt tagcgaaaga cgttcagaca gcattgcagc
    59461 acagcattcg agaaataggt ctgcgtttga tgcgaatgaa aaatgatggg atgagtcaga
    59521 aggatattgc agccaaagaa gggctgtctc aggctaaggt cacgcgtgct ctccaggcag
    59581 cgagtgctcc ggaagaatta gtcgcccttt tccctgtgca gtcggaatta accttttcgg
    59641 actacaaaac gctttgtgct gttggcgacg aaatggggaa caagaattta gagtttgatc
    59701 agcttattca aaacatatcc ccggaaataa acgacatctt atccattgaa gaaatggccg
    59761 aagatgaagt taaaaataaa atcctgcgct tgataacaaa ggaagcctca ctactcacgg
    59821 ataaaggttc taaagataag tccgtagtta ctgaattatg gaaatttgag gacaaggatc
    59881 gctttgcaag gaagcgcgtg aaaggccgtg cattttctta tgagtttaat cgactttcaa
    59941 aagagctaca ggaagaactc gacaggatga ttgggcatat ccttagaaag agcctcgata
    60001 aaaagccgaa gtcttaaact ttcgccattc aaatttcact attaacctac tgtttttaaa
    60061 gtaaatccat ctaaaatttc aaggtgaaat cgccacgatt tcaccttgga ttttaccttc
    60121 ctcccctact cccgaaaaaa taaaaaaatt gcttgtcacg agaaagtcaa caagtgactt
    60181 tcaataaaat ctcttccgaa aagggattca cacaagtgcc ttgtgtttaa ggaagagtaa
    60241 attgagtaac ttacgcgaat accagaatcg tattgcagat atcgcaaaac gctctaaagc
    60301 tgtgcttggc tgggcaagca ctgcgcagtt cggtactgat aaccaattca ttaaagatga
    60361 tgccgcgcgt gccgcatcta tccttgaagc tgcacgtaaa gacccggttt ttgcgggtat
    60421 ctctgataat gccaccgctc aaatcgctac agcgtgggca agtgcactgg ctgactacgc
    60481 cgcagcacat aaatctatgc cgcgtccgga aattctggcc tcctgccacc agacgctgga
    60541 aaactgcctg attgagtcca cccgcaatag catggatgcc actaataaag cgatgctgga
    60601 atccgtcgca gcagagatga tgagcgtttc tgacggtgtt atgcgtctgc ctttattcct
    60661 cgcgatgatc ctgcctgttc agttgggggc agctaccgct gatgcgtgta ccttcattcc
    60721 ggttacgcgt gaccagtccg acatctatga agtctttaac gtggcaggtt cctcttttgg
    60781 ttcttatgct gctggtgatg ttctggacat gcaatccgtt ggtgtgtaca gccagttacg
    60841 tcgccgctat gtgctggtgg caagctccga tggcaccagc aaaaccgcaa ccttcaagat
    60901 ggaagacttc gaaggccaga atgtaccaat ccgaaaaggt cgcactaaca tctacgttaa
    60961 ccgtattaag tctgttgttg ataacggttc cggcagccta cttcactcgt ttactaatgc
    61021 tgctggtgag caaatcactg ttacctgctc tctgaactac aacattggtc agattgccct
    61081 gtcgttctcc aaagcgccgg ataaaggcac tgagatcgca attgagacgg aaatcaatat
    61141 tgaagccgca cctgagctga tcccgctgat caaccacgaa atgaagaaat acaccctgtt
    61201 cccaagtcag ttcgttatcg cggctgagca cacggtacag gcggcgtatg aagcacagcg
    61261 tgaatttggt ctggacctgg gttccctaca gttccgcacc ctgaaggaat acctgtctca
    61321 tgaacaggat atgctgcgtc ttcgcatcat gatctggcgt actcttgcga ccgacacctt
    61381 tgacatcgct ctgccggtta accagtcctt taatgtatgg gcaaccatca ttcgtggcaa
    61441 attccagact gtatatcgcg acattattga gcgcgttaaa tcttctggtg cgatggggat
    61501 gtttgctggt gctgatgcag catctttctt caaacagttg ccgaaggatt tcttccagcc
    61561 agccgaagac tatatccaga ctccgtatgt tcactacatc ggtaccctgt tcggtaacgt
    61621 gaaagtgtac gaagtacctg ctggtatttg taagaactta acgacagaga acattcagtt
    61681 cagctcgatg gatgtgctgt gctacgtccg tgatgaaaat ccgggtaaag caggcttcgt
    61741 gactggtgat gctgtcccgg ctatcccgtt ccagcatccg accactccgg cgctggtcaa
    61801 ccgtaccaca ctgtggggtt cggctatcaa cgatatgcac ccacgcaacg gcgctgatta
    61861 cttcactcgt gtaacgctga caatggccaa aaaaggcggg cttaacttca taagcggcga
    61921 cacgattgat gccggtgact ctgagtaatc aggggaagtt ctccgtttaa catagcgccc
    61981 ccgtgcgggg cgcataacag ggaaagttat gtctcaatat tcaattcaac agtcattagg
    62041 taatgcatcc ggcgtcgcgg ttagcccgat caatgccgat gcgacgttat ctaccggtgt
    62101 tgcattaaat agcagcttgt gggctggtat tggcgtattt gcgcgtggca agccgtttac
    62161 tgttcttgcg gttactgagt ccaattacaa agatgttctc ggcgaaccgc tgaagccgtc
    62221 ttccggctca cagttcgaac caattcgcca tgtatacgaa gctattcagc aaacgtcagg
    62281 ttatgttgtt cgtgctgttc cggatgatgc gaagttcccg attattatgt tcgatgaatc
    62341 aggcgaaccg gcttacagtg cgttgccata cggttctgaa attgaacttg atagtggcga
    62401 agcctttgct atctacgttg atgatggtga tccgtgtatt tcacctaccc gtgagttaac
    62461 catcgaaacg gcaacagcgg acagcgcggg taatgaacgc ttcctcttaa aactgaccca
    62521 gacgacttcg ctcggcgtgg taacgaccct ggagacacac actgtgtctt tggcggaaga
    62581 agcgaaagat gacatgggcc gcttgtgtta tctgcctacg gctctggaag cccgttctaa
    62641 atatctgcgc gcggttgtta atgaagagct gatttcgacg gcgaaagtaa caaataaaaa
    62701 atcgctggcg ttcactggtg gtaccaacgg tgatcagtcg aaaatctcaa ccgctgcgta
    62761 cctgcgtgcg gttaaggtgc tgaacaatgc gccgtacatg tacaccgctg ttctcggcct
    62821 gggttgctat gacaatgcgg cgatcaccgc gttaggtaat atctgttctg atcgcctgat
    62881 tgatggcttc tttgatgtca aaccgacatt gacgtatacg gaagcgctct ctgctgttga
    62941 agataccggt ttacttggta ccgattatgt aagctgtgct gtctatcact acccgttctc
    63001 ctgcaaagac aaatggaccc aatcccgtgt ggtctttggt ctgtctggcg tggcgtatgc
    63061 ggcgaaagct cgtggcgtca agaaaaactc tgatgtcggc ggttggcatt actcaccggc
    63121 tggtgaagaa cgtgccgtca ttgctcgtgc gtcaattcaa ccgctgtatc cggaagatac
    63181 cccggacgaa gaagcaatgg tcaagggccg tctcaataaa gtatctgttg gcacctctgg
    63241 ccagatgatc atcgacgatg ctttaacttg ctgcacgcag gataactatc tgcacttcca
    63301 gcacgtccca tccctgatga atgcaatcag ccgtttcttt gtccagttag cccgacagat
    63361 gaagcatagc ccggacggta ttactgcggc tggcctgact aaagggatga ccaaactttt
    63421 agatcgcttt gtcgcctccg gcgctctggt ggctcctcgt gatcctgatg ctgacggtac
    63481 agaaccgtat gtgctgaaag ttacgcaggc ggaattcgat aaatgggaag tagtctgggc
    63541 ctgctgcccg actggcgtag cccgtcgtat ccagggcgta ccgctgctta ttaagtaagg
    63601 gaatacaatg agcaaaaact tttttcaatc cggggcattt ttggggaatg gactgtctcg
    63661 tttcgctttg aactctgatc ctgtgcagct gatggagtct gcccgagcaa gcgccgaaca
    63721 gccaacagat ccggttatta ataataatcc ggaaccggcg gcacagacta acgataacgt
    63781 tccatctgcc ccggctcctg agcaaatcct ggaagggaaa gacggtaaag aatggaccgt
    63841 cgaacaggcg caccagatga ttctggaagc tgcaaataga agtgctatgc agaatgcgtt
    63901 gagtgatgcg gccgacgccg ttttcgcctg ggctgatagc ggtgatctga ctttcgactc
    63961 ccttgatggt ttcgttcagg ctatcgctgg tatctctgat gacgacgact ccgaagttac
    64021 agaagaacag gacgatgcct ataacgaagc atgggcaaat gttgctgact tcctcgcagc
    64081 atgcggtgta gatgatgacc tgatcgaagc actggctgac gatgaagacg acgacgcagc
    64141 tgctgatgtt ggtgcctcta tcgctggttt agatagcgac gaccgtgacg aactggaagc
    64201 ggcgtttgtt gttgctggca cttctgatga aatgctgact gaagcattta agaaggttgt
    64261 tcgtaacggt gagatcaaac tcatccgtaa acgcctgcgt aaaaaacgtc tgactgcggc
    64321 tcaaaaatcg gcgctgaaaa aagcgcgtcg aaaagcccag acgggtgcgg caaaacttgc
    64381 ccgcaaaaag tcaatgaaac tgcgccgtaa gcgccttggc taaaggagga ggccggagaa
    64441 ctccggcctt taacttgaat ggcacctata ccttatgggg tttacagcca ggctgacggt
    64501 gtatcgccat ttctgaaagt tactttaacg aactctcagt accaggttac cggatatatc
    64561 agccaggggg cagcaatgaa catggcccag aattgggaag cgccgtttac cggtatgtcc
    64621 atggggtctg ttgctggtgc cttcagtggt tttgcgcagg ttggtactga aacaacgtcg
    64681 gtggcccgtt ggaacagctt aatggtttgg gaggggggaa caccgccaac tttcacgctg
    64741 ccagtaactt tcatcgcttt gtttgatcca ttcacggagg tttcaggcgc tatcgccgca
    64801 ttgtcagcga tgattagccc ggaacttaaa gatgccagta ttggtggtcg aatcccggag
    64861 cgtgtgacgc taaacattgg tcgccagatc aacatcattg atgtcgctat ccaggacata
    64921 agtttcgatc tcgatgcgcc cagggacagc aatgggcatt tcctgaaaaa caccgtcaac
    64981 ctccagttga ccggttcttc gatatataac agctccgata ttgttcgggc gttccagtaa
    65041 aaggatttta tatggggcac aataacacta agggaaaccg taaatttatt aagggccgct
    65101 atactgccaa cgcggccaaa ggcgaacgac tggtatcttc tgaattccag ctcactgttg
    65161 caggccatga agatatcagc gtactggttc gcacgtcgca aattcctgaa atgacccgcg
    65221 aggatgtgga ggactatggt ccgaatggtg tgaagttcaa ccagcacgga ccaattcgaa
    65281 actctgggga aatccaggtc cagtgcgtgg agactatcga aggcgatatt cttcagttca
    65341 tcaaggatcg cattgcggcg aaggactatg ttgatatcac gatggctgct acccctgaat
    65401 ccaaatcttc cggggttaac gctgtgacaa aagctgctac aacaattgaa atgttggact
    65461 gcaaaatcta cagtgatgca atcgacttta gtaccgaaga tgtgactgcc gctgtgcgcc
    65521 cgtcacttcg tatcgtctac aactggattg agtgggatta atagtaatcc cttgtatttt
    65581 aaagctcctt cgggagcttt tttatttgga gaggaaaggg tgcattgagg atacctgaca
    65641 cacgaagagt ggcggggatc tctccccgcc aggtctctta cctttcagat tcgtaggctg
    65701 tgaagacagt gacctccgtc tggccggttc ggattcgtac ctcgcagagg tctttcctcg
    65761 ttaccagtgc cgtcacaatg acggttaaac agatgacgat cagagcgatt aacatcgctt
    65821 tttgctgctt catagcctgc ttctccttga ccttttggtc ggtaagaggc taatctacgt
    65881 atgctaagca tagatgtggc ctcagattaa tgttaagcgt cttgcaggac gcgtaatgtt
    65941 atctggggct ttcttctatc tgcttttcgg gtaatgcctg aagcagatag cctcaagcac
    66001 ccgcaacgat tgtatcaatg tctggctttt tttctataga aatcacctgg aagggtgaat
    66061 atccacatca gaagaaatgt tgcagcaaac atgatcccta atggccagac cgcgccaaag
    66121 aaaatccata ctaagatctc ctctgcttgt tctttgcggt cgatatcgac aagcattttt
    66181 cggctgatca tgtatacaca gaagccaata caaacatatc ctgcaaaagc gatcgctaac
    66241 tgtaaaaaat cagattgcat ctccgacctc aaactgaaaa cgccaggtga ctccagatta
    66301 gagcaatcta tcaccctctg aatcctgccg gtatacccca ttgttcgtta tctttatttt
    66361 tggctaaaac cgcattaaga gcttcgttta ccgtcatgca atgcggcaga ttatcgaagt
    66421 ttgatacccc gccaatatca ggagaacgct tgttcttcag gtaagcatat ttccgcgctg
    66481 ccgcctctac tttctgcttg aactcatgtt tttgagcgcg ttttttggat aaccgcagat
    66541 tgtcagcctt tgcttttgcc tcagcgatcc atgaagtcaa ttttttgagt ttggtcgttc
    66601 cggcagcgcc ggaaactgat ctttttgttt ttttaaattg tgacttctta ttctttattg
    66661 ccacgtcatc ctgacagggg gagggggtat cattttgaca tgggggtgtg gataaaaaat
    66721 taaataaagc caatgtctta gcgagaacag ctttaacctt ggttgccgct gaagagatct
    66781 ttaatttgct ttcaatcagc gcattttttg cttgttgtgc gaaggccaaa aaggatggtg
    66841 taaaccggta caggttagcg cgacgttcac ggtgatcgcc gataacaatc tctactgaca
    66901 ggattccttt gtttacagct tcacggaatg cacgaacgac ggttgattgg ctataaccag
    66961 tttctgccgc gatcaggcgg tgaggcttgt gaatgaagta ttcactggtt gttgccgcga
    67021 gatttgcaca ttgcgacagg atatgcccgg cgctacggga tagaccggag tgtgttacaa
    67081 agcaggccaa ttcatagcca gaaaaagtaa aatcgctcat cgttatacag ctcaggaaag
    67141 tgactttagc cagcattaca atgctggtgg ttcttactac gtctgttagc gcgctgccgc
    67201 gacaggtacc agcacaccag catcaagcaa tcgcttcatc agccactgct gacctttgcc
    67261 ggttatacga gtcgtgaaag aaatcctgct tccattgctt gtatcgatca cggtttcttt
    67321 aagggtgaaa tacccacggg atatgtattc ttgtttgggg acgttcctgc gttcaccggt
    67381 tgcgatcaga attccgttat cacgcaacca ggtgaagaga tagttttggc ccaggccgag
    67441 cactttggca tagttgccga ttagaactcc gctggcggta gcaacgcgtt cggcgaattc
    67501 gactttaggt gcatccataa gcattttttg ctccagccgt tgcttttgct ctgccaggtc
    67561 ggcagccaaa cggagagctt cagggagact ctgcggaata gcaggttgta atcttccagc
    67621 tcgatagtcg ataaatgtct ggtttacctt cagccgaaac gcgggagaaa tccagcctgc
    67681 gtactccaca gcgagcaatt catgggcaaa agtgccgccg ccacggcctt cgaacgaaac
    67741 tatgcaattc tgcatagttt ctttttcaag ctcttcgatg agctgtttgg ctgacagcgt
    67801 tcttagccat tgagctggcg ctttatgggc accgagtccg ctcgctctgt gtagagcatt
    67861 aaggttgtaa cggccagcgc ggtcggtcgt aatttcaaca ccacaaataa caggcagagt
    67921 ggttgaagga tcgacatttt gatgaaggtt tgatatattc atatccgcat tgaatgtttg
    67981 ttgcattttt tctccaaatt tgcatcaacc ttcaatcacc agctcgaaat ggtgattctt
    68041 tgcacttaga aaacgaaatt tattagagca aatttttctg actcgatcca gatcgggttg
    68101 gtcgatctgc tcagaaacct gccagtttgc tggcaggttt tttcttttgt taacctattg
    68161 ctactggttt taacaaacca gcatcaagta gcttgcgagt taaccactgc tggcctttcc
    68221 ccgttaattg aggcgtcaac cgtatctggt agccatcttc atcatccagc accacttctt
    68281 tcaccgtgaa atacccggca tttatgtact gctgaaacgg tacattttta cgtccgccgg
    68341 acgcaatcag gatgccgttc tcccgtaacc aggcaaacag cgcgttttgc ttaagtccaa
    68401 caacctttgc aaaattccca atcaggatcc ccttggccac tgatacccgg tcggcaaaat
    68461 cgactttagg ggctgcggcc accagctgtt gttccagttg cattttctgt tctgccaact
    68521 cggcagccag gcgcagagct tctggaagcg tctggggaat tgagaccggt tgctgttctt
    68581 tttgtcggaa gtagctgtct tccagttttt caaagaatgc ccatgcttca tttgtgtcca
    68641 cgatcttaga catgcgtgca gcgccgcgct ctgtccagag aattacctga cttgtatatt
    68701 tgctaaccaa gtgactaata gtcactagcc ttttaaattc ctttaacttt gaaccggtta
    68761 agagatagta atgcttgcct tcttcaaagc gatcggagtt gcgagacaaa tttttacgaa
    68821 tattggcttc atcggtacca taccctctag caagagtttc ggtcgttacg acacgcactc
    68881 ccagccattc cagaacggga atttcatcag actgattctg aacaaccacc agctccgatt
    68941 cccgaactga aggtgcataa attttttctg atttaacgtt agttgctttc attctgtgtg
    69001 cctccttgcg tgcttcggct gcgacggttg cgtaattcag atgaccctgt tcgagcaggt
    69061 attcacggat atcggttagc agaatgcggt gaaccgcgtg tttgtccttt ctccggtaaa
    69121 gttgtttggt gatcatgaag tagttggcaa taacgcccgg tatatccctg gtactgatac
    69181 aggcagtgtg ctgttcaatt gcctcgatca tctcttcacg ggtgactaat gacgttctca
    69241 tagtccctcc tgagcagaag cgttaacagg gaggcaccag taactgagag aattgcgcga
    69301 atcggtagaa aagcgggcag agaaaataca gggtgcatcc ggaagctgag agcgagcctc
    69361 atcttctgtc ggtgcaataa cgaagtgata gtgacgtttc tggcaggagt aaaagcgcca
    69421 gataaattca ggatgagttg gggtagggat agtagccaca atggcagcct ccttttgcta
    69481 atttaaggag ctaccgcgtg aggttccaat ctcaatggcg gtagcactga ctgggttgga
    69541 actaccggcg caaaagggaa ccggcctgcc tttcggcagc ccagccagca ctaccattga
    69601 tcttgtagct atgtgctacg tatggttgtg cgatggcatg acataaaaaa gacgcttttg
    69661 gcgtctgtgt cgccttttgc attatccggg gttccaatcc cggcatccgt tttactaaga
    69721 tgcttgttta ggataaaccg aaaatgcatt agcacgcaag cgagttaatg tagcatatga
    69781 aactaaaaaa acaaaataat tagagcaatt tgtgtccgta taaattatta gctatgcaac
    69841 taaaatgtat aactctaatt gatatggatc aaagactttg accattgcat ggtgaaactt
    69901 tgtgctacaa aatgttattt taaggcgttt tatctgtggc tggagatagt taatcatgtc
    69961 atctgaacat gttcgtaaag gtgtaacaaa tgcgaaattc aatgaagagc aatctaatat
    70021 tctctttatt gaaataggaa ttctgtcaat actgataggt ttaatgtcta aatcatggtg
    70081 ggcctttggc ggttcatttc ttggactgat ttttagttta agaataaaat ttctggcaat
    70141 accattgatg attgtcttta gcttagtatg gggagctatt ggctatagca ttggtgcttt
    70201 gtttgaatca acagcagcta gtattgtttt gggaataatt gctttcctga gtggcttggg
    70261 cactcatttt gctgccgtgc aatgggcaaa tgatattgca gaataattac agataagaag
    70321 cgaaaaaaat gaatatgaga tggtttttat tatttgtatc gttaatttca gcaggagtat
    70381 ttgcaaaaat tgatcctcta caatggaatg taattgaaga
//
LOCUS       DAZFWL020000006        69804 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFWL020000006 DAZFWL020000000
VERSION     DAZFWL020000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 69804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.006"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            75..593
                     /locus_tag="ACHKNP_RS02370"
                     /old_locus_tag="pgaptmp_000474"
     CDS             75..593
                     /locus_tag="ACHKNP_RS02370"
                     /old_locus_tag="pgaptmp_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU4702702.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            609..1148
                     /locus_tag="ACHKNP_RS02375"
                     /old_locus_tag="pgaptmp_000475"
     CDS             609..1148
                     /locus_tag="ACHKNP_RS02375"
                     /old_locus_tag="pgaptmp_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HGU4702703.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKRVGKAEENKSDSWFN"
     gene            complement(1243..2820)
                     /gene="guaA"
                     /locus_tag="ACHKNP_RS02380"
                     /old_locus_tag="pgaptmp_000476"
     CDS             complement(1243..2820)
                     /gene="guaA"
                     /locus_tag="ACHKNP_RS02380"
                     /old_locus_tag="pgaptmp_000476"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGU4702704.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2889..4355)
                     /gene="guaB"
                     /locus_tag="ACHKNP_RS02385"
                     /old_locus_tag="pgaptmp_000477"
     CDS             complement(2889..4355)
                     /gene="guaB"
                     /locus_tag="ACHKNP_RS02385"
                     /old_locus_tag="pgaptmp_000477"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGU4702705.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4517..5887
                     /gene="xseA"
                     /locus_tag="ACHKNP_RS02390"
                     /old_locus_tag="pgaptmp_000478"
     CDS             4517..5887
                     /gene="xseA"
                     /locus_tag="ACHKNP_RS02390"
                     /old_locus_tag="pgaptmp_000478"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGU4702706.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5884..6099)
                     /locus_tag="ACHKNP_RS02395"
                     /old_locus_tag="pgaptmp_000479"
     CDS             complement(5884..6099)
                     /locus_tag="ACHKNP_RS02395"
                     /old_locus_tag="pgaptmp_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU4702707.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6168..7640)
                     /gene="der"
                     /locus_tag="ACHKNP_RS02400"
                     /old_locus_tag="pgaptmp_000480"
     CDS             complement(6168..7640)
                     /gene="der"
                     /locus_tag="ACHKNP_RS02400"
                     /old_locus_tag="pgaptmp_000480"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGU4702708.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7758..8936)
                     /gene="bamB"
                     /locus_tag="ACHKNP_RS02405"
                     /old_locus_tag="pgaptmp_000481"
     CDS             complement(7758..8936)
                     /gene="bamB"
                     /locus_tag="ACHKNP_RS02405"
                     /old_locus_tag="pgaptmp_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HGU4702709.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8947..9567)
                     /gene="yfgM"
                     /locus_tag="ACHKNP_RS02410"
                     /old_locus_tag="pgaptmp_000482"
     CDS             complement(8947..9567)
                     /gene="yfgM"
                     /locus_tag="ACHKNP_RS02410"
                     /old_locus_tag="pgaptmp_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HGU4702710.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9585..10859)
                     /gene="hisS"
                     /locus_tag="ACHKNP_RS02415"
                     /old_locus_tag="pgaptmp_000483"
     CDS             complement(9585..10859)
                     /gene="hisS"
                     /locus_tag="ACHKNP_RS02415"
                     /old_locus_tag="pgaptmp_000483"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGU4702711.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10970..12088)
                     /gene="ispG"
                     /locus_tag="ACHKNP_RS02420"
                     /old_locus_tag="pgaptmp_000484"
                     /gene_synonym="gcpE"
     CDS             complement(10970..12088)
                     /gene="ispG"
                     /locus_tag="ACHKNP_RS02420"
                     /old_locus_tag="pgaptmp_000484"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HGU4702712.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12115..13128)
                     /gene="rodZ"
                     /locus_tag="ACHKNP_RS02425"
                     /old_locus_tag="pgaptmp_000485"
     CDS             complement(12115..13128)
                     /gene="rodZ"
                     /locus_tag="ACHKNP_RS02425"
                     /old_locus_tag="pgaptmp_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGU4702713.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13413..14567)
                     /locus_tag="ACHKNP_RS02430"
                     /old_locus_tag="pgaptmp_000486"
     CDS             complement(13413..14567)
                     /locus_tag="ACHKNP_RS02430"
                     /old_locus_tag="pgaptmp_000486"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HGU4702714.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14717..15148)
                     /gene="ndk"
                     /locus_tag="ACHKNP_RS02435"
                     /old_locus_tag="pgaptmp_000487"
     CDS             complement(14717..15148)
                     /gene="ndk"
                     /locus_tag="ACHKNP_RS02435"
                     /old_locus_tag="pgaptmp_000487"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGU4702715.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15297..17609)
                     /gene="pbpC"
                     /locus_tag="ACHKNP_RS02440"
                     /old_locus_tag="pgaptmp_000488"
     CDS             complement(15297..17609)
                     /gene="pbpC"
                     /locus_tag="ACHKNP_RS02440"
                     /old_locus_tag="pgaptmp_000488"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HGU4702716.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGAWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NANRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNSILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17610..22571)
                     /gene="yfhM"
                     /locus_tag="ACHKNP_RS02445"
                     /old_locus_tag="pgaptmp_000489"
     CDS             complement(17610..22571)
                     /gene="yfhM"
                     /locus_tag="ACHKNP_RS02445"
                     /old_locus_tag="pgaptmp_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HGU4702717.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPSETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNHHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANDVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22778..23623
                     /gene="sseA"
                     /locus_tag="ACHKNP_RS02450"
                     /old_locus_tag="pgaptmp_000490"
     CDS             22778..23623
                     /gene="sseA"
                     /locus_tag="ACHKNP_RS02450"
                     /old_locus_tag="pgaptmp_000490"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HGU4702718.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23959..24084
                     /gene="timP"
                     /locus_tag="ACHKNP_RS02455"
                     /old_locus_tag="pgaptmp_000491"
     CDS             23959..24084
                     /gene="timP"
                     /locus_tag="ACHKNP_RS02455"
                     /old_locus_tag="pgaptmp_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HGU4702719.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24116..24892)
                     /gene="sseB"
                     /locus_tag="ACHKNP_RS02460"
                     /old_locus_tag="pgaptmp_000492"
     CDS             complement(24116..24892)
                     /gene="sseB"
                     /locus_tag="ACHKNP_RS02460"
                     /old_locus_tag="pgaptmp_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HGU4702720.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPGFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25034..26317)
                     /gene="pepB"
                     /locus_tag="ACHKNP_RS02465"
                     /old_locus_tag="pgaptmp_000493"
     CDS             complement(25034..26317)
                     /gene="pepB"
                     /locus_tag="ACHKNP_RS02465"
                     /old_locus_tag="pgaptmp_000493"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HGU4702721.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26495..26695)
                     /gene="iscX"
                     /locus_tag="ACHKNP_RS02470"
                     /old_locus_tag="pgaptmp_000494"
     CDS             complement(26495..26695)
                     /gene="iscX"
                     /locus_tag="ACHKNP_RS02470"
                     /old_locus_tag="pgaptmp_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HGU4702722.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26707..27042)
                     /gene="fdx"
                     /locus_tag="ACHKNP_RS02475"
                     /old_locus_tag="pgaptmp_000495"
     CDS             complement(26707..27042)
                     /gene="fdx"
                     /locus_tag="ACHKNP_RS02475"
                     /old_locus_tag="pgaptmp_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HGU4702723.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27044..28894)
                     /gene="hscA"
                     /locus_tag="ACHKNP_RS02480"
                     /old_locus_tag="pgaptmp_000496"
     CDS             complement(27044..28894)
                     /gene="hscA"
                     /locus_tag="ACHKNP_RS02480"
                     /old_locus_tag="pgaptmp_000496"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HGU4702724.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28911..29426)
                     /gene="hscB"
                     /locus_tag="ACHKNP_RS02485"
                     /old_locus_tag="pgaptmp_000497"
     CDS             complement(28911..29426)
                     /gene="hscB"
                     /locus_tag="ACHKNP_RS02485"
                     /old_locus_tag="pgaptmp_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HGU4702725.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29522..29845)
                     /gene="iscA"
                     /locus_tag="ACHKNP_RS02490"
                     /old_locus_tag="pgaptmp_000498"
     CDS             complement(29522..29845)
                     /gene="iscA"
                     /locus_tag="ACHKNP_RS02490"
                     /old_locus_tag="pgaptmp_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HGU4702726.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29862..30248)
                     /gene="iscU"
                     /locus_tag="ACHKNP_RS02495"
                     /old_locus_tag="pgaptmp_000499"
     CDS             complement(29862..30248)
                     /gene="iscU"
                     /locus_tag="ACHKNP_RS02495"
                     /old_locus_tag="pgaptmp_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HGU4702727.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30276..31490)
                     /gene="iscS"
                     /locus_tag="ACHKNP_RS02500"
                     /old_locus_tag="pgaptmp_000500"
     CDS             complement(30276..31490)
                     /gene="iscS"
                     /locus_tag="ACHKNP_RS02500"
                     /old_locus_tag="pgaptmp_000500"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGU4702728.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31731..32219)
                     /gene="iscR"
                     /locus_tag="ACHKNP_RS02505"
                     /old_locus_tag="pgaptmp_000501"
     CDS             complement(31731..32219)
                     /gene="iscR"
                     /locus_tag="ACHKNP_RS02505"
                     /old_locus_tag="pgaptmp_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HGU4702729.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32520..33257)
                     /gene="trmJ"
                     /locus_tag="ACHKNP_RS02510"
                     /old_locus_tag="pgaptmp_000502"
     CDS             complement(32520..33257)
                     /gene="trmJ"
                     /locus_tag="ACHKNP_RS02510"
                     /old_locus_tag="pgaptmp_000502"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HGU4702730.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAAQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33376..34179
                     /gene="suhB"
                     /locus_tag="ACHKNP_RS02515"
                     /old_locus_tag="pgaptmp_000503"
     CDS             33376..34179
                     /gene="suhB"
                     /locus_tag="ACHKNP_RS02515"
                     /old_locus_tag="pgaptmp_000503"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HGU4702731.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34324..35178
                     /locus_tag="ACHKNP_RS02520"
                     /old_locus_tag="pgaptmp_000504"
     CDS             34324..35178
                     /locus_tag="ACHKNP_RS02520"
                     /old_locus_tag="pgaptmp_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4702732.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35369..36649
                     /gene="csiE"
                     /locus_tag="ACHKNP_RS02525"
                     /old_locus_tag="pgaptmp_000505"
     CDS             35369..36649
                     /gene="csiE"
                     /locus_tag="ACHKNP_RS02525"
                     /old_locus_tag="pgaptmp_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HGU4702733.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36641..37780)
                     /locus_tag="ACHKNP_RS02530"
                     /old_locus_tag="pgaptmp_000506"
     CDS             complement(36641..37780)
                     /locus_tag="ACHKNP_RS02530"
                     /old_locus_tag="pgaptmp_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HGU4702734.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37940..38830)
                     /gene="hcaR"
                     /locus_tag="ACHKNP_RS02535"
                     /old_locus_tag="pgaptmp_000507"
     CDS             complement(37940..38830)
                     /gene="hcaR"
                     /locus_tag="ACHKNP_RS02535"
                     /old_locus_tag="pgaptmp_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HGU4702735.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38966..40327
                     /gene="hcaE"
                     /locus_tag="ACHKNP_RS02540"
                     /old_locus_tag="pgaptmp_000508"
     CDS             38966..40327
                     /gene="hcaE"
                     /locus_tag="ACHKNP_RS02540"
                     /old_locus_tag="pgaptmp_000508"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HGU4702736.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40324..40842
                     /gene="hcaF"
                     /locus_tag="ACHKNP_RS02545"
                     /old_locus_tag="pgaptmp_000509"
     CDS             40324..40842
                     /gene="hcaF"
                     /locus_tag="ACHKNP_RS02545"
                     /old_locus_tag="pgaptmp_000509"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HGU4702737.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40842..41162
                     /locus_tag="ACHKNP_RS02550"
                     /old_locus_tag="pgaptmp_000510"
     CDS             40842..41162
                     /locus_tag="ACHKNP_RS02550"
                     /old_locus_tag="pgaptmp_000510"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HGU4702738.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41159..41971
                     /gene="hcaB"
                     /locus_tag="ACHKNP_RS02555"
                     /old_locus_tag="pgaptmp_000511"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41159..41971
                     /gene="hcaB"
                     /locus_tag="ACHKNP_RS02555"
                     /old_locus_tag="pgaptmp_000511"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HGU4702739.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41981..43183
                     /gene="hcaD"
                     /locus_tag="ACHKNP_RS02560"
                     /old_locus_tag="pgaptmp_000512"
     CDS             41981..43183
                     /gene="hcaD"
                     /locus_tag="ACHKNP_RS02560"
                     /old_locus_tag="pgaptmp_000512"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HGU4702740.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43280..43702
                     /locus_tag="ACHKNP_RS02565"
                     /old_locus_tag="pgaptmp_000513"
     CDS             43280..43702
                     /locus_tag="ACHKNP_RS02565"
                     /old_locus_tag="pgaptmp_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU4702741.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43750..44622)
                     /locus_tag="ACHKNP_RS02570"
                     /old_locus_tag="pgaptmp_000514"
     CDS             complement(43750..44622)
                     /locus_tag="ACHKNP_RS02570"
                     /old_locus_tag="pgaptmp_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HGU4702742.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44634..45728)
                     /locus_tag="ACHKNP_RS02575"
                     /old_locus_tag="pgaptmp_000515"
     CDS             complement(44634..45728)
                     /locus_tag="ACHKNP_RS02575"
                     /old_locus_tag="pgaptmp_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU4702743.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45761..46759)
                     /locus_tag="ACHKNP_RS02580"
                     /old_locus_tag="pgaptmp_000516"
     CDS             complement(45761..46759)
                     /locus_tag="ACHKNP_RS02580"
                     /old_locus_tag="pgaptmp_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4702744.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46784..48295)
                     /locus_tag="ACHKNP_RS02585"
                     /old_locus_tag="pgaptmp_000517"
     CDS             complement(46784..48295)
                     /locus_tag="ACHKNP_RS02585"
                     /old_locus_tag="pgaptmp_000517"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU4702745.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIIAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(48318..49301)
                     /locus_tag="ACHKNP_RS02590"
                     /old_locus_tag="pgaptmp_000518"
     CDS             complement(48318..49301)
                     /locus_tag="ACHKNP_RS02590"
                     /old_locus_tag="pgaptmp_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU4702746.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGGDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49398..52679)
                     /locus_tag="ACHKNP_RS02595"
                     /old_locus_tag="pgaptmp_000519"
     CDS             complement(49398..52679)
                     /locus_tag="ACHKNP_RS02595"
                     /old_locus_tag="pgaptmp_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HGU4702747.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNQEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52797..53990
                     /locus_tag="ACHKNP_RS02600"
                     /old_locus_tag="pgaptmp_000520"
     CDS             52797..53990
                     /locus_tag="ACHKNP_RS02600"
                     /old_locus_tag="pgaptmp_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGU4702748.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(54054..55307)
                     /gene="glyA"
                     /locus_tag="ACHKNP_RS02605"
                     /old_locus_tag="pgaptmp_000521"
     CDS             complement(54054..55307)
                     /gene="glyA"
                     /locus_tag="ACHKNP_RS02605"
                     /old_locus_tag="pgaptmp_000521"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGU4702749.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55635..56825
                     /gene="hmpA"
                     /locus_tag="ACHKNP_RS02610"
                     /old_locus_tag="pgaptmp_000522"
     CDS             55635..56825
                     /gene="hmpA"
                     /locus_tag="ACHKNP_RS02610"
                     /old_locus_tag="pgaptmp_000522"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HGU4702750.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56870..57208)
                     /gene="glnB"
                     /locus_tag="ACHKNP_RS02615"
                     /old_locus_tag="pgaptmp_000523"
     CDS             complement(56870..57208)
                     /gene="glnB"
                     /locus_tag="ACHKNP_RS02615"
                     /old_locus_tag="pgaptmp_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HGU4702751.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57269..58603)
                     /gene="glrR"
                     /locus_tag="ACHKNP_RS02620"
                     /old_locus_tag="pgaptmp_000524"
     CDS             complement(57269..58603)
                     /gene="glrR"
                     /locus_tag="ACHKNP_RS02620"
                     /old_locus_tag="pgaptmp_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HGU4702752.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58593..59306)
                     /gene="qseG"
                     /locus_tag="ACHKNP_RS02625"
                     /old_locus_tag="pgaptmp_000525"
     CDS             complement(58593..59306)
                     /gene="qseG"
                     /locus_tag="ACHKNP_RS02625"
                     /old_locus_tag="pgaptmp_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HGU4702753.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59471..60898)
                     /gene="qseE"
                     /locus_tag="ACHKNP_RS02630"
                     /old_locus_tag="pgaptmp_000526"
     CDS             complement(59471..60898)
                     /gene="qseE"
                     /locus_tag="ACHKNP_RS02630"
                     /old_locus_tag="pgaptmp_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HGU4702754.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCICRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61474..65361)
                     /gene="purL"
                     /locus_tag="ACHKNP_RS02635"
                     /old_locus_tag="pgaptmp_000527"
                     /gene_synonym="purI"
     CDS             complement(61474..65361)
                     /gene="purL"
                     /locus_tag="ACHKNP_RS02635"
                     /old_locus_tag="pgaptmp_000527"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGU4702755.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGLGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65619..67175
                     /gene="mltF"
                     /locus_tag="ACHKNP_RS02640"
                     /old_locus_tag="pgaptmp_000528"
     CDS             65619..67175
                     /gene="mltF"
                     /locus_tag="ACHKNP_RS02640"
                     /old_locus_tag="pgaptmp_000528"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HGU4702756.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(67172..67675)
                     /gene="tadA"
                     /locus_tag="ACHKNP_RS02645"
                     /old_locus_tag="pgaptmp_000529"
     CDS             complement(67172..67675)
                     /gene="tadA"
                     /locus_tag="ACHKNP_RS02645"
                     /old_locus_tag="pgaptmp_000529"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGU4702757.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(67733..68368)
                     /gene="yfhb"
                     /locus_tag="ACHKNP_RS02650"
                     /old_locus_tag="pgaptmp_000530"
     CDS             complement(67733..68368)
                     /gene="yfhb"
                     /locus_tag="ACHKNP_RS02650"
                     /old_locus_tag="pgaptmp_000530"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HGU4702758.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68577..69425
                     /locus_tag="ACHKNP_RS02655"
                     /old_locus_tag="pgaptmp_000531"
     CDS             68577..69425
                     /locus_tag="ACHKNP_RS02655"
                     /old_locus_tag="pgaptmp_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGU4702759.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69481..69741
                     /gene="yfhL"
                     /locus_tag="ACHKNP_RS02660"
                     /old_locus_tag="pgaptmp_000532"
     CDS             69481..69741
                     /gene="yfhL"
                     /locus_tag="ACHKNP_RS02660"
                     /old_locus_tag="pgaptmp_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HGU4702760.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
BASE COUNT        16558 a        19257 c        17610 g        16379 t
ORIGIN      
        1 tacgcaatac atttactttc ctcttttgat gatcttaaat gtcttatttt tcgtaatgtg
       61 tataacaagg aatagtgatg aaatttaaaa aatgtcttct gcctgtggca atgttagcgt
      121 cattcactct ggcaggatgc cagtcaaatg ctgacgatca tgccgccgat gtttatcaaa
      181 ccgatcaact gaataccaaa caagaaacta aaaccgttaa tattatttcc attcttcccg
      241 caaaagttgc cgtagacaac tcccaaaata aacggaacgc acaagccttc ggcgcgctta
      301 ttggcgcagt cgctggcggt gttatcggcc acaacgtcgg ttctggcagc aattccggaa
      361 cgacggcagg ggcagttggc ggcggagctg taggcgcggc agtgggttct atggtgaatg
      421 ataaaacctt agtggaaggt gtttctttaa catataagga aggcaccaaa gtgtatacct
      481 ctacccaggt gggtaaagag tgccagttta cgacaggttt agccgttgtt attaccacaa
      541 cgtataacga aacgcgtatt cagccaaata ctaaatgtcc tgaaaagagc taataatcag
      601 gaggagtcat gaagaaagtt tttctttgcg ccatcttggc ctccttaagc tatccggcta
      661 tcgcctcatc attgcaggat caactctctg ctgtagcaga agctgaacag caaggtaaaa
      721 atgaagagca aaggcagcat gacgaatggg tcgcggagcg caacagggaa atccagcaag
      781 agaagcaacg tcgcgcaaat gcccaggccg ccgctaacaa aagagcggca acggcagcgg
      841 caaataagaa agctcgtcag gataaactgg acgccgaagc ctctgcggac aaaaaacgcg
      901 atcaaagtta tgaagatgag ctacgcagct tagagattca gaaacaaaaa ctggcgctgg
      961 caaaagaaga agcccgcgtt aagcgagaaa acgaatttat cgatcaggaa ctgaagcaca
     1021 aagctgcgca aaccgatgtg gtgcaatctg aagctgacgc caacagaaat atgactgaag
     1081 gcggtcgcga tctgatgaaa cgcgtgggca aagcagaaga gaacaaatcg gacagctggt
     1141 ttaattaatc gatgttagta acttcaagcc tatgattctt gaagataaaa aaccctctgt
     1201 agtaacagag ggttttgttc attcatagtg cagggattaa aatcattccc actcaatggt
     1261 agctggcggc ttgccgctga tgtcatacac cacgcgggaa ataccgttca cttcattgat
     1321 aatgcggttg gaaacgcgac cgaggaaatc gtacggcaga tgcgcccagt gtgcggtcat
     1381 aaagtcgatg gtttcgacag cacgcagaga gacaacccaa tcatacttac gaccatcgcc
     1441 cattacgcca acggaacgta ccggcaggaa cacagtgaac gcctggctga ctttgtcgta
     1501 caggtccgct ttacgcagtt cttcaatgaa gatggcgtca gcacggcgca gcaggtcaca
     1561 gtactctttc ttcacttcac ccagcacacg aacgccaagg cctggtcccg ggaacgggtg
     1621 acggtacagc atgtcgtacg gcaggcccag ctccagacca atcttacgca cttcgtcttt
     1681 gaacagctct ttcagcggtt caaccaggcc catcttcatc tctttcggca ggccgcccac
     1741 gttgtggtga gatttgatga cgtgtgcttt accggttgca gaagccgcag attcgataac
     1801 gtcagggtag atggtgccct gcgccagcca cttcacgtct tccagtttca gcgcttcttc
     1861 atcgaatact tcaacgaaaa cgcgcccgat gattttacgt tttgcttccg gatcgttttc
     1921 gccagccagc gctgacagga agcgatcttc tgccggtacg tgaacaatgt taagaccaaa
     1981 gtgatcgcca aacatatcca gaacctgctc tgcttcgttg agacgcagca ggccgttgtc
     2041 gacgaatacg caagtcaggt ttttaccgat agcgcggtgc agcagcattg cggttacgga
     2101 ggaatccaca ccaccagaga ggccgaggat gactttatcg tcgcctacct gctcgcggat
     2161 gcgagctaca gcatcgtcga taattttcgc tggcgtccac agggcttcac actggcagat
     2221 atcacgcaca aaacgctcca gcatgcgcat accctggcgg gtgtgagtca cttccgggtg
     2281 gaactgtacg ccatagaagc gtttttcttc gttagccata atggcaaacg ggcagctttc
     2341 ggtgctggct acggtgacga agtcggacgg gatagcggta actttatcgc cgtggctcat
     2401 ccagacatcg agcagcggtt taccgtctgc ggtcagcgca tcttcgatac cgcgaaccag
     2461 tgcgctgtcg tttacgactt caacctgcgc gtagccaaat tcacgttcgt tagaggcttc
     2521 aacgtgaccg cccaactgca ttgccatggt ctgcatgcca tagcaaacgc cgaataccgg
     2581 tacgcctgct tcaaagacat actgcggcgc acgcgggctg ttttcttcgg tggtgctttc
     2641 cgggccgccg gaaagaataa tgccgcttgg attgaagtca cgaatttgtg cttctgtcac
     2701 atcccacgcc cacagttcgc agtaaacacc cagctcacgc acgcggcgcg caaccagttg
     2761 agtgtactga gaaccgaagt ccagaatgag gatgcgatgc ttatgaatgt tttccgtcat
     2821 tgacgcttat tccgaggcaa gtgaaacaga taatataaat cgcccgacat gaagtcgggc
     2881 gaagagaatc aggagcccag acggtagttc ggggactctt tagtaatggt cacgtcgtga
     2941 acgtggcttt cctgaatgcc cgcaccgctg atacgtacaa actccgcttt agtacgcagt
     3001 tcgtcgatag taccacagcc ggtcagaccc atacaggagc gcaggccgcc catctgctgg
     3061 tgaatgatct ctttcaggcg acctttataa gctacgcgac cttcgatacc ttccggcacc
     3121 agtttgtcgg cagcgttatc gctctggaaa taacggtcag aggaaccttt ggacatcgcg
     3181 cccagggaac ccataccacg gtaagatttg taagaacggc cctggtagag ttcgatttca
     3241 cccggagatt cttcagtacc cgccagcatg gaacctacca tcaccgcgct tgcgccagct
     3301 gcgatagctt tggcgatgtc gccggagaag cgaataccac catctgcgat aaccggaata
     3361 ccggtgcctt ccagggcttc aactgcgtca gcaacggcag tgatctgcgg aacacctacg
     3421 ccagtaacga tacgagtcgt acagatagaa ccagggccga taccgacttt aaccgcgctg
     3481 caaccagctt ctgccaaagc gcgtgcacct gcagctgttg ccacgttgcc gccgatgatt
     3541 tgcagatccg gatatttagc acgggtttca cggatacgct gcaaaacgcc ttctgagtga
     3601 ccgtgggagg agtcgatcag cagaacgtca acgccagcgg caaccagcgc gtcaacacgc
     3661 tcttcgttac ccgcacctgc gccaactgca gcaccaacac gcagacggcc ttgctcgtct
     3721 ttacaggcgt tcggtttacg ttccgctttc tggaagtctt tcacggtgat catgccgatc
     3781 aggtggaatt cgtcatcaac caccagcgct ttttcaacgc gtttttcgtg catttttgcc
     3841 agcaccactt cacgggcttc accttcacgc acggtgacca gacgctcttt cggcgtcatg
     3901 taaacgctaa ccggctggtt caggtcggta acaaaacgca cgtcacgacc ggtgataata
     3961 cccaccagtt cgttttcttc ggtaacgacc ggatagcccg caaaaccgtt acgctcggtc
     4021 agttctttca cttcgcgcag cgtcgtggtt ggcagaacag tctgcggatc agtcaccaca
     4081 ccagattcgt gttttttcac acgacgaact tcttctgcct ggcgttcaat ggacatgttt
     4141 ttgtggataa agccgatacc gccttcctga gccagagcaa tagccaggcg cgcttccgtt
     4201 acggtatcca ttgctgcgga aagcataggg atattcagac gaatagtttt cgtcagctgg
     4261 gtgctgaggt cagcagtatt cggcagaacg gtagaatgag caggaacgag gagaacgtcg
     4321 tcaaacgtca gagcttcttt agcgatacgt agcatgggca atatctcgac cagagtggtt
     4381 aataaatatt gccgcggcat tatacagagc gtaaccgatt gcatctaccc ctttttgcaa
     4441 aaaatgcttg ctatccccga agggcgggtt actatcgact gaataacctg ctgatttaga
     4501 atttgatctc gctcacatgt taccttctca atcccctgca atttttaccg ttagtcgcct
     4561 gaatcaaacg gttcgtctgc tgcttgagca tgagatggga caggtttgga tcagcggcga
     4621 aatctctaat ttcacacaac cggcttccgg tcactggtac tttacactca aagacgacac
     4681 cgcccaggta cgctgcgcga tgttccgcaa cagcaaccgt cgggtgacct tccgtccaca
     4741 gcatgggcaa caagttttag ttcgcgccaa tattacgctc tacgagccgc gcggcgacta
     4801 ccagataatc gttgagagta tgcagccggc cggtgaaggg ctgctgcaac agaagtacga
     4861 acagctcaaa gcgaagttgc aggctgaagg attgttcgat ctgcaataca aaaaaccact
     4921 tccctcccct gcgcattgcg ttggtgtgat cacctcaaaa accggtgctg cgctacatga
     4981 tattttgcat gtgttaaaac gtcgcgatcc ttctctaccg gtgatcatct accccaccgc
     5041 cgttcagggc gatgacgcgc cggggcaaac tgttcgcgcc attgaactgg cgaatcagcg
     5101 caatgagtgc gacgtgttga tcgttgggcg cggcggcggt tcgctggaag atttatggag
     5161 ttttaacgac gaacgcgtag cgcgggcgat ttttgccagc cgcattccgg tcgtcagcgc
     5221 cgtcgggcat gagacggatg tgaccattgc cgattttgtt gccgatctgc gtgcgccaac
     5281 gccgtctgcc gccgctgaag tagtgagccg taatcagcaa gagttactgc gccaggtgca
     5341 atcgacccgt caacggctgg agatggcgat ggattattat ctcgccaacc gcacgcgtcg
     5401 ctttacgcag atccatcacc gattacagca acagcatccg cagctccggc tggcacgcca
     5461 gcaaaccatg cttgagcgcc tgcaaaagcg gatgagcttt gcgctggaaa atcaacttaa
     5521 gcgtaccggg caacagcagc agcggttaac acagcggctg aatcagcaaa atccacagcc
     5581 gaagattcat cgcgcgcaaa cgcgcattca gcaactggaa tatcgtttag cagaaaccct
     5641 gcgcgtacag cttagcgcca cgcgtgaacg tttcggtaat gcagtaacgc acctcgaagc
     5701 cgtaagccca ctgtcaacgc tggcgcgtgg atacagcgtt actactgcta ctgacggcaa
     5761 tgtactgaaa aaagtgaagc aagttaaagc gggtgaaatg ctaaccacac gtctggaaga
     5821 cggctggata gaaagtgaag taaaaaacat ccagccggta aaaaaatcgc gtaaaaaggt
     5881 gcattaagcc agcacgaatt caacgcgttt tttcgatatc aagccatgcc cgtgctgaca
     5941 gaagtaatct actgcaccgc aggcttttaa cacctgcaac ggttgatggc agtctggaca
     6001 aagagctttc atttcaataa tttccccgca ggatgggcaa cgcgcatggt cgttatcctg
     6061 atcaagcaca tgctgacatt gtggacaatg cagttccata tgaattcctc cggtagccat
     6121 ccctctacat tcatagaggg aatggcagat aaaatactta cggataatta tttatttttc
     6181 ttgatgtgct tcatcagacg cttacgttta cgcatctggg ttggcgtcag ggtgttacgc
     6241 ttgttcgcat acgggttttc cccttctttg aactgaatac gaatcggcga tcccattacg
     6301 tccagcgatt tgcggaagta gttcatcaag tagcgcttgt aggaatcagg caggtctttc
     6361 acctgattac cgtgaatcac cacaatcggc gggttataac caccagcgtg ggcatatttc
     6421 agcttcacac gacgaccgcg taccagcggc ggttggtgat cttcaacagc catcgtcatg
     6481 atgcgcgtca gcatagaggt ccccacacga cgggtggagc tgtcatacgc ttcacgtact
     6541 gattcaaaca agttaccaac accactgccg tgcaaggcag agataaagtg cacacgagca
     6601 aaatcgataa agcccagacg gaagtccagc gtttctttca cctgctcttt cacttcctgg
     6661 ctcaggccat cccacttatt caccacaatg acaagtgagc gcccactatt gagaataaag
     6721 cccagcagcg agagatcctg atcggaaata ccttcgcgcg catcaatcac taacatcacc
     6781 acgttggcat cttcaatggc ctgcaacgtt ttgattacgg agaatttctc tacagcatcg
     6841 gtgattttgc cgcgtttacg tacgccagcg gtgtcaatga gcacatactc acgtccatcg
     6901 cgttccattg gaatgtagat actgtcacgc gtcgtgccag gcatgtcata aacaacaacg
     6961 cgctcttcac caagaatacg gttagtgagt gtagacttac ctacgttcgg acgacccaca
     7021 atcgccagtt tgatcggcag actttgcggg tcgaagtcgt cttcctcttc ttcttcgccg
     7081 ttctcttccg cttcaaattg cgcccagtat tcagcgtctt cgtcgacttc ctcttgcggt
     7141 gcgagatctt ccatccacgg cagcagcaca tgctccagca gacttaatac gccacgaccg
     7201 tgagacgcgg cgatcgggta gatttcacct aaaccaagcg agtagaaatc aaccactgcc
     7261 tgatcgggat ccagaccgtc agttttgttt gccaccagga aggtcggttt ttcacgggag
     7321 cgcagatgtt tggcaatcgc ttcatctgcc ggcatcaggc ccgcgcgcgc atccaccata
     7381 aacagtacga cgtccgcttc ttcaatcgcc agcagcgact gttccgccat gcgggtttct
     7441 acaccgtctt ctgtgccatc aatcccgccg gtatcaatac agataaactc acggccttca
     7501 atttccgcac gaccgtactt acggtcacga gtcagacccg ggaaatccgc aaccagcgca
     7561 tctcgggtgc gagttagacg gttaaataac gtggattttc ctacgttagg gcgcccgaca
     7621 agcgcgacca caggtaccat gtttaaagcc tcatttttat aaatcatcag acaacgcacg
     7681 ctatattcgc gtcgttgtta aaaacaggaa aacggcccct gtccaggagc cgttttcaaa
     7741 gtgaacgaca gagacgatta acgtgtaata gagtacacgg ttccgtcttt tgcctggatc
     7801 agcagtttgc cgtcagcggc aaccggttca gtctggaaac cggaactatc aactttttgc
     7861 tgggcaacga aacgaccatc ttcgacgtta atccagtgca gataaccttc actgtcacct
     7921 accaccaggt tgccattata cagcaccgga gaagtcagca ggcgatgcaa cagatcgctt
     7981 tgtgtccaca gcgtaacgcc gccatcaatg gtcaacgcca tcacccggtc attttgatcg
     8041 accagataga tgcgattgcc gtcgacgatg aaatcattca ccgaacccag ttcgcgtttc
     8101 cacataatct gaccactgcg cagatcaagc gccgtcaggt taccattata ggccagcgcg
     8161 aaaacaacgc cgttaacaac gacgggagtc gtgtcaacat cgctcagacg gtcaatttcg
     8221 gtagaaccgg tcgcctggga aatacgctgc tgccaaatca tctggccctg ttccatcagc
     8281 actgcgctga cgcgaccatt atcgccaccc acgacggccg caccaaaagc cgttgccggc
     8341 gcagactcgc cacgcaaaga gagcgaaggc atatcgaggt taactgtcca tttgacagcg
     8401 ccgtcagcct cgttcagcgc ttgtaactga ccgttactgg tgtggattaa caccagaccg
     8461 tcgctgacca ccgggcgcga aagtgcttca cccgcgactt tagtttgcca tgccacagta
     8521 ccatcgctgg tattcagcgc gtaaacctgc gccttttcgc tgccaatgta gacatgccca
     8581 ccagacacgg tcagaccgcc agaaagtaat gcaggctctt tagagaacca gccatctttc
     8641 tcggccaggc tgacagacca gatttctttg ccatcatccg cattcagcgc ttttaccaaa
     8701 ccagcgcggt ccgctgcata gacaacgttg tccgccagtg ccggatgaag attggaatag
     8761 aagttgccaa tgccgctacc aacggaagtg ctccacgccg tggtcggcgt aaactggttt
     8821 tcaacggttg gcaatgggga catctttacc acatcttctt cgctgttaaa cagcgaacag
     8881 ccgcttaaaa gggtaacgga aagcagtcct ggcagcagta atttacgcaa ttgcatcggg
     8941 tccctctcag atggacaaat tattaatttt catctgcatc atttcgctca gtgccggagt
     9001 aacatcgctt ttcacgcctg cttcccatgc actacgcgca ccttgcttat cacctttgct
     9061 cagcaatgct tcaccacgca ggtcggcaac aatggcagcc cacccttcac ctttgatggt
     9121 atcaagggtt ttcagcgcgg catcagcctg cttgagctgt acctgaacgc gagcaagacg
     9181 cagatttatc acggctttga gattttcatc gctcgtgtct gccagcccct gttgtaactg
     9241 ggcggcagct ttctctagtt catttttgtc aacaaattgc tgcgcaagtt ccaaagaagc
     9301 cagcgcacca taagtatttt tattttcagc agcaaatttt tccgccgccg ggatgctatc
     9361 cggtttgcct tcgctcactg cggtaaccgc attttgatag gcaagagaag cggagcgtgc
     9421 agaatcaacc tgatggctgt tccagtagcg ccagccaatc agtgcgccaa cgcccaaaat
     9481 caccccaaca gccagtgctt tgccattttc agcaaaaaag cgtttaaccg cttctacctg
     9541 gtcgttttcg ttctcgtaaa tttccacgct gtccttctcc ttccttaacc cagtaacgtg
     9601 cgcaaatgcg cggctacgct atcctgcgca actgccgttt gctcaccaga gcgcaaatcc
     9661 ttcactactg ctgtgccgtt agccacttca gactcaccca gcaccacagc aacgcgggca
     9721 ccccatttat cagcacgggc aaactgtttc ttaaagttgc cgccgccgtg gttggtcatc
     9781 aatttcacgc ccggtaattc atcacgcaga cgctcagcta atgccatagc cgcagattgt
     9841 gtatcagcac ctgaagccac caggtatata tcgacaacag gatcggcttt aaattccgga
     9901 ttaacggcct gtactaacaa tacaagacgt tcgaggccca tcgcaaaacc gacagccggt
     9961 gttgcacgac cgcccagttg ttccacaaga ccgtcataac gaccgcctgc acacacggtg
    10021 ccctgggagc cgagactgtt agtcacccac tcgaaaacgg tacggttata gtaatccaga
    10081 ccacgcacca gacgctggtt tacggtgtaa gcgatccccg cgctctcaag cagtttgcac
    10141 agaccggcaa aatgctcacg agattcctcg tccagataat cacctaatgc cggagcgtcg
    10201 ttgagaagcg cctgcacttc cggatttttt gaatccagca cgcgcagcgg gttagtgtac
    10261 atgcggcgtt tgcagtcttc gtccagcttt tctttatgct gctcaaggaa tgccaccagc
    10321 gcatcgcggt aattggcgcg tgcttccagc gaaccgatag aattcagctc aagagttacg
    10381 tgttcggaaa tacccagcgc gcgccaccag cgggcggtga gcataatcag ttcagcgtcg
    10441 atatccggac cttgcagacc gaaaacttcg cagcccaact gatggaactg acgataacgc
    10501 cctttctgcg gacgctcgtg acggaacatc ggcccgatat accacagacg ctgttcctga
    10561 ttgtacagaa gaccatgctc gatgccggcg cgtacacagc ccgccgtccc ttcagggcgc
    10621 agagtcaggc tgtcgccatt gcgatcctca aaggtgtaca tctctttttc aaccacgtcg
    10681 gtgacttcac caatcgcacg tttgaatagc ggggtctgct ctacaatcgg caagcggatt
    10741 tcactgtaac cgtagctgcc gagcacgttt ttcagtgtgc cttcaatgcg ctgccagatg
    10801 gccgtttcgc caggcaggta atcgttcatg ccgcgaatgg cttgaatgtt ttttgccacg
    10861 tttattctct ttctgaatat aaaaatgaac cctcaacgct tccctcaatg tttcgggagc
    10921 tatgcgggtt caatcataca cgggaagcga ggcgcttccc atcacgttat tatttttcaa
    10981 cctgctgaac gtcaattcga cgcgcttcgt ccagctgact ggctttcgca cgaatgcgtg
    11041 cttccagctg gtcgatcata tcgttgttgt ccagacggtc tttgcgcacg ccatcttcat
    11101 agaggccgct tttcttgttg ccgccggtga cgccgagtgt agaaaccagc gcctcacctg
    11161 ggccattcac cacgcagccg ataatcgaaa cgtccatcgg agtgatgata tcttccaggc
    11221 gttgctccag cgcgttaacc gtaccgataa catcaaattc ctgacgcgaa caggtcgggc
    11281 aggcgatgaa gttgatcccg cgcgaacgga tacgcagcga tttcaaaata tcgaaaccga
    11341 ctttgatctc ttcgaccgga tcggccgcca gtgatacgcg cagcgtgtcg ccgatgcctt
    11401 cagacagcag cagacctaaa ccaatggcgg attttactgc cccgctgcgt gcaccaccgg
    11461 cttcggtgat ccccagatgc agcggctgat cgatctgttt tgccagcaaa cgataagact
    11521 caacagcgag gaagacgtca gacgctttca cgctgacttt gaactgatcg aagttcaggc
    11581 gatcgagatg atcaacatgg cgcatggcgg attccagcaa cgcctgcggc gtcggttcgc
    11641 catacttttc ttgcagatct ttttccagcg atccggcgtt aacgccaata cggatcggaa
    11701 tgtttttatc gcgcgcacag tcaaccacca tgcgaatacg ctcttcatta ccgatattgc
    11761 cagggttaat acgcagacaa tcgacgccgt attccgctac tttcagtgca atgcgatagt
    11821 cgaagtggat gtcagccacc agcggcacgt taacctgctg tttgatgagt ttgaacgctt
    11881 ctgccgcgtc catcgtcggt acggagacac ggacgatatc agcgccaacg cgttccagcg
    11941 ccttgatttg attgaccgtt gcttcgacgt ctgtcgtacg cgtattggtc atggactgta
    12001 cggcgatggg agcaccatcg ccaatcggca cattcccaac gtaaatacgt gttgattttc
    12061 tacgttgaat tggagcctgg ttatgcatga aaaatctccc gcgttacccg tctgttactg
    12121 cgccggtgat tgttcggcat tgagggtcag acgcgcaacc tggttagttc tgataaaacg
    12181 actcagatcg acaggtttcc cttgatactg gatctgtact gcggctggcg caccaatttt
    12241 cagtttgtat ggtgcctggc cggttaagtt caaattaccg tctttacgct gcataccgct
    12301 aaacaatttt ttaccggtag catcagtaac ctccagccag caatcggcag taaagttcat
    12361 caccagcgca ttcggatcag ccgccggcgt agtcacgcct gcctgatcgg ttggcaacgg
    12421 cgcagcgcca tctggcgttg ttgttgccgt tggtgccggg gtcgcggcgg tatcaacatt
    12481 tgcctgcgaa ggcgaaacaa ccgcattctg ttgcggatca acagctggtg ctggcgcagt
    12541 tacggcaggt gtttgcgtgt tggttgcggt agtatccaca gacgctggcg gcgtggacgt
    12601 tgtagccggg tctgtagttg tcgacgtatt taacggaaca ctctgcccct gctcgctatt
    12661 actgctcagt tccgccgaag attgatcggc catagtggtg atctcttcct gctgagcttt
    12721 gtggtcttgc caccaccagg caccgctcag gccgataacc acaaacaaca ccagccaggt
    12781 gaaggtcatc agccagccgt cacgtttttt gcggcgttta ccgagggaaa aactctgcat
    12841 aggcgcaact tttgcagccc gtagtggagc ctgcttttcc agccctggca gcagttcttc
    12901 ttctggaata tgtaccagac gcgcataaga gcggatatat ccgcgcagga atgttgaagc
    12961 aagatcggcg ggtgccttat cttcttcaat gtcgcgtacc gtggaaacct tcaggcaaag
    13021 tcgctcggca acggcctgct gactaagtcc tagttgttcg cgagcattac gcaggcgagc
    13081 gccggtagta agtgcttcat tttggtcgtg cgtggcttca gtattcattc gctgcaggta
    13141 cgtttaaatg agaattgaga tgccggtgaa ccatcatgct cacccacacc gcgaaacatc
    13201 cgttaagtta accgttatca tacagtataa gaccgtctgt gtgctcgtga caaagcccac
    13261 attcactaag gctaacttac tgttgcatcg ttacatactg ccttaaagtc agcaaaaacg
    13321 caccgttaat attgaccaga caattgcaac ttaatacttc attcataaaa gattacgcca
    13381 cggtacataa agtaaccgtg gcgtaatggc tatcagaccg ctttaatgtc gatggcttca
    13441 ccctgcatcc gtttacgcag ggtacgtttc gtacggtcga taacatcgcc cgccaactga
    13501 ccacaggcag catcgatatc atcaccacgg gttttacgca caatagtggt gaaaccgtag
    13561 ctcatcagca cttttgagaa acggtcgata cggctgttcg agctgcgtcc atacggcgcg
    13621 cccgggaagg ggttccacgg gatcaggttg atcttacacg gcgtatcttt cagcagttcc
    13681 gccagttggt gcgcgtgttc agtgccgtcg ttaacgtggt caagcatcac atactcaata
    13741 gtgacacggc cctgattggc gttggatttc tccagataac ggcgcaccgc agcaaggaac
    13801 gtttcgatat tgtacttttt gttgatcggc acaatttcgt cacgaatttc gtcgttcggc
    13861 gcgtgcaggg aaattgccag tgcaacgtcg atcatatcgc ccagtttatc cagcgccgga
    13921 actacaccgg aagtagaaag cgtgacgcga cgtttagaca ggccaaaacc gaaatcatca
    13981 agcatgattt ccatcgccgg aacgacgttg ttcaggttga gcagcggctc gcccatgccc
    14041 atcatcacta cgttagtgat tgggcgctga ccggtgactt ttgctgcgcc gacgattttc
    14101 gccgcacgcc acacctggcc aataatttcc gatacccgca ggttgcggtt aaagccctgc
    14161 tgggcggtgg aacagaattt acactccagc gcacacccca cctgcgaaga gacgcagagc
    14221 gtggcacggt cgtcttccgg gatatacacc gtttcgacgc gctgatcgcc aacggcgatc
    14281 gcccatttaa tggtgccgtc ggatgaacgc tgttcttcaa ccacttccgg tgcgcggatt
    14341 tccgccacct ctttcagttt gccgcgcaac actttgttga tgtcggtcat ctcatcaaag
    14401 ttgtcgcagc aatagtgata catccacttc atcacctgat cggcgcggaa gggtttttca
    14461 cctaaatctt taaaaaactc ccgcatctgc tgacggttga gatccagcag gttgattttt
    14521 ccatctttcg tggtgacgtt ttcaggtgtg actaattgtt cagacatatg ctattccggc
    14581 ctcgttatta cacgttatgg cccctggagg attgaaaaaa gaaacgcccc ggtgagcggc
    14641 tgctcgtccg ggggcgctgc attgtacaaa ttctggcgca cggatgccac gtttgcacgc
    14701 ggcatttacg aaattattaa cgggtgcgcg ggcacacttc gccttcgcca aagaaataag
    14761 cgatttcgcg agcggcagat tcgacggaat cagaaccgtg ggtaccgttt tcggtcaggc
    14821 tgtcagcgta atcagcgcgc agagtaccag ccagtgcgtt tgccggattg gtcgcgccca
    14881 gcagatcgcg gtgacgctga acggcgtttt caccttccag cacggaaacc acgatcgggc
    14941 cagaggtcat gaattcaacc agaccatcaa agaacggttt tccatcgtgt tcagcataaa
    15001 agccacgtgc ctgttcaacg gtcaggtgca gcattttggt gccgacaatt ttgaaccctg
    15061 cagcttcaaa gcgcgcaaag atattaccaa tgacgttttt tgctaccgcg ttcggtttga
    15121 tgatggaaaa agtacgttca atagccattt ttacctctgt aaattgttct gttgttgtct
    15181 gtaccagcgt acgagatggc gcggattata atgagcaaca gggccgttga ctattgatga
    15241 aggtaacatt tttttaaaat aaaatgagtt ttagcaacaa tcagggacaa aacagactat
    15301 tgcatgacaa atttcactgt cgcgatttgc cccacatcat ccatcaccag caattgataa
    15361 tcacctttat ccgtcaaatg cagggtcacg ttgcgcccgc gttcagttaa cggttcgcca
    15421 ttcagaaacc accagcgttc acctgcccct ccacttgatt gcaacggcaa agtcgcttcc
    15481 gctgcgcccg gtaaacgttt aataatcgcg ccatcgcgaa cgcctgtcag ctgcagaggg
    15541 agctgggcat cgtgaccgta tgacgggcaa attgtcgagg ccggtggtaa gcgcacagcg
    15601 cgacgttctg atgcgggcag ccagggttcc agcggtagcg gccagacatt tatcatttct
    15661 tgtcgcgctt gcgggcaatc agcggcaaca cgtttgccat tttcatccag ccagatgggg
    15721 aaacgaatgc cattaatgcc ttcctgctcc ggcagtaata gagttggcgg ctgactcccg
    15781 tccagcagcc aggttgccag gcggcggcga cagttaccgt caccttccgg caaagactgt
    15841 ccgcccggcc agcagataac gccacgagtg actgagttcg ggcgcgggtc ttccggcaga
    15901 ttcgcactgc gcgacagtaa gatactattg acctgattca acaatggtac ggcactggca
    15961 aagccaaact gaccaacaac gggcgtgccg tccggtctgc cagtccagat cccaatgaca
    16021 tagcgagcgt taaccccaat cgcccaggcg tcacgatagc catagctggt gcccgttttc
    16081 catgccagtg gggcgacgcg cggcaaggca ctatccggca agggtttcgc ttcatcagcc
    16141 ataatccggc gaatgatcca cgccgccccc gacgacatta aaggccgttc aagcagcgga
    16201 tcgtcaggct gtaagcgcaa tttgcctgcc ttgccgtggc gagcaaacgc ggtatacgct
    16261 gccgccatat cttccagttt tgcaccagcg ccgccgagaa tgagtgaaag attcggcgca
    16321 gcaccgttgg gcaaatataa cggcaatcca acattgcgta actttgccgc aaaccgtttc
    16381 ggtccatagg cttccagcac ctgcacagca ggtaagttca gcgagcgcac cagcgcctcg
    16441 ctcatgctga tcgggccatg aaaaccgcta tcaaagttac ctggtcgata atcaccggtg
    16501 cgccggggga cgtcttgcag cagtgatgcc gggtggatca agccttcatc cagcgccaga
    16561 ccataaacaa acggtttgag caccgatcct ggcgatcggt tcgcattgac catatcaaca
    16621 tgaccaaagc gtgaatcatc gttgagatca accgatccca cccagccgcg aacacgcata
    16681 tcggtatgat caaccacgat catcgccagt gagctgcgcg gtggcaaccg ccctttccag
    16741 ttttgcgcca gttcttccag acgtcgttga agaccggcat ccagcgtagt aacgattttg
    16801 tcgcttttgc ttttaccgag catcatgcgc gaaaacagcg gtgccagttg cggcatttgt
    16861 cggggtgcca gccagatggg ttcttccctt gactctttta cctgctcacg ggaccacgca
    16921 ccttgcacgg ccatccgttc gagcacttta ttacgcgcgg cttcggcacg ctccggccaa
    16981 cgatccgggc gaagacggct gggcgcttgc ggcaaaaccg ccagcattgc cgcctcggaa
    17041 tagcttaaat tcgcaggcga ctttccgaga taagcccaac ttgccgcacc gatcccctgc
    17101 aacgtaccgc caaacggagc gcggttaaga tacaaggtca gaatttcacg cttagacaga
    17161 tgccattcca gttgcaacgc gcgccagagc tggcgaattt tgccgccaaa tgttttgggg
    17221 tgaggatcaa gcagacgagc aacctgcata gtgagcgtgc tgccaccgga aataacccgt
    17281 cccgaagtga gatcttgcca tgctgcgcgc gccaccgaga atggattcac ccccggatgc
    17341 ttccagaacc agcgatcttc ataattgatc agcgcttcaa ggtaacgtgg agaaacatct
    17401 tcgattgtta ccggataacg ccagatgccg tcagcatcgg cgaagcgcca tagcggcgta
    17461 ccatcctgcg ccacgaccac tcgtgcggga ttgacttcat gcagcggtag aggccagagt
    17521 ttatctgctc cccacgctgc aagaaaaagg agaaatggcg cagctgccag cgttatccag
    17581 cagccgcgtt tggttaacaa gcgaggcatt tacggtcgga caatcagcag atcttcagcc
    17641 gcgccggtcg cccgccattg gggaacatac attgattcca ccatcggttg cggtacctga
    17701 tacgttcccg gcgtcaccgc ccgcgccaga tacaccagcg ttaccggttg gtattcatca
    17761 acggcaaccg ccgccacaaa gcgatcgtca cggaactcaa tgtgcttaat actcgcctgc
    17821 tgcatctggt tcagtaagtt ctgcacttcg ccaccacttt gctccaggct ggcgctaccg
    17881 ttcgccagat tctggttttc cagttccaga cccgcaggca gcagatccac gaccaacgca
    17941 tccggcacgc tgttactggc ttttacctgc aaccacacca gcaccagatc gccgctacgt
    18001 aacgagtcca gcgatttgct cttaccatca gtaccaagaa tatgacgctc gatttgcagc
    18061 acatcgttcg ccggtaaagg tgcggattgc ggataaccgc tggcatccac acgcagccat
    18121 aacggctgat cgccactgtt tctcacctgc aaggtggcaa gttgatcgct attcagattg
    18181 ctgtttagcg ttttctcgcc tgtcagcggc tcagctgaga aagaggtttg cgcctgccat
    18241 ttacccggta aatcctgaat cgtacgggca gccaggaaca acgcgttact ttcctgcgtc
    18301 gatagccagc gttcaccaaa tgcctgctgc gaaagcgtgt tcagcaaagt gttctgctca
    18361 tcgggtagca gtttattttc ttccagcaag gagagcatta acgcgttgtc gcgcagtgga
    18421 ctaccgtaat cacccagcca tatccgctca tcactatttc tcggcgtttt cagcgccagc
    18481 acaatcgctt cttcaccacg cgttgcatca cccatggttt tcagcgcaac gccaagttgc
    18541 agcagcggta aaccagaagc ggcatctgca cgatgctccc agatttcacg cagcgcaccc
    18601 agtggtgctt tttgctgacg ggccagcacc agcgcagcgt aagactgaac ggcgaattta
    18661 ctggctttga gattatccgc gtacgggatc gacatcatgc ccggatcttg taaatagcgc
    18721 agcagacgct cattaccccg gttaatggcg tctgtcggca cgctgtaacc ctgttcgcct
    18781 gcgcggacca ggaaatccat cacgtaagcc gtcagccagt actcttcgtc accgttttta
    18841 tcccacagcg caaagccgcc gttatcacgt tgcatttgca gcaaacggga aatgccgata
    18901 tcgaccgatg cacggcgttt ctcatcactg tcgcctttga tgcccaacgc ctgcagttgg
    18961 gctgcgttgg tataaagtga cgggaacagg ccgctggcgg tttgctcaag acagccgtac
    19021 ggatacgctt ttaactcttt gatataacgt gcaatgttca gcggtggttt gccgctcaac
    19081 aacaattgcc cttccagcgt aacaggcgag aagttttgca atccatccgc cggaatcgcc
    19141 catgtctcac caggctgtaa cgccgtaccg taattaaccg tttgtgccgg gaacgccgga
    19201 cggacgccga ttttccactg cttatgctga tcggcaacgg tttcacccgg taacgctaac
    19261 ccgctaatgg tggcctgaat ttctccatcg ccataacccg gcaatgctcg caccgggata
    19321 aacagcgtag tacgcacacc tggcgctaat tcaacggctg cgggtgaatc gctgaccagt
    19381 tcaagcaaac cactggcggt cagggcaacg ttcagttttt gcggtttatc ggtaagatta
    19441 gtgatatcca gcgtcagacg cgaggtatcg ccactcgcca taaagcgcgg catgttcagt
    19501 tcagcaatca ccggtgcggc aactatcact ttgctttcgt tgctaccgaa atcatctgcc
    19561 gtccaggctt gcgccatgac gcgcaattca ccgttaaaat cgccaatcgg cagtgtaacc
    19621 gagccttcgc cctgttcgtt gagcgttacc ggcagcgcct gctgcgcgac aatattgacg
    19681 tgattgaccg gcggtttacc accacgtttc agctcatcac catcgccacc gaaacgcaga
    19741 gctgccagac gcccctgacc ttcaataacc tgaccgtaaa tatcgtaaat gtctgcgcca
    19801 tagcgtttct gaccaaagaa cgcctgccac ggatctggcg tgacgtagtc agtaatattc
    19861 agcacaccac tatcaacggc agacaccagc acattcacct gtttaggctt ctcgccattt
    19921 ttagtgctgg ctttaatttt cacggttaat ggttgattgg gacgcatttt tgctggtgtt
    19981 tccagcgcca gatcgaggcg acggttttca tcgccaagcg gcagatgcaa cacaccaacc
    20041 gcgcgttttg gcgtcgcgga gcgagattta tcgccaggac gtaccaccag cgtacttaaa
    20101 tacagatcat gatgattcca ggttttatcg accggaatcg tcagatccag cccttgagcc
    20161 cgaacatcaa tctcttgcca ccacagcggc ccttcactgg actcgaccat cgcataacct
    20221 ttacccgccg ttggcgcggc gatatgcaac ttaatggtgt cgccagggcg ataactggct
    20281 ttatccagtt tcagcgtgac acggtcgggt cgcactgcgc cgctaccgtc gctgttgtcc
    20341 tgccagctat agccagccca gaaacgaaca ctactgaccg cttcattcgg cgctttgact
    20401 tccagacgat aagcgcccca ctctaccgga aaactgacct tgccggtttc gtccgctttc
    20461 agatccagag tttgttcatt ttcgatcaga tctttttgat caaactgtga ctgccagcct
    20521 tcatcttctg accagttcca gtagtaatcg cggcgttcgc gaatcaggcg cacctgcaag
    20581 cccgacacgg cttttttcac gccttgcgca tcgctataaa cgatgtcaaa agcggcgtta
    20641 ctgccttcat caacaatggg ctgtttcacc gtgctgtcag tacgataatc gtaaaccgat
    20701 ttcgaggcga actgcggacg gatccccggc aatgcatcgg caggccagat agcctgctca
    20761 gcgcggcgcg tcaccgggcg accgcccgat tccagcaaac taccctggaa aataacctgt
    20821 aatggggaat gcgtttcctt ccactggctt tctgtagaaa cttcgccgcg ccctttatca
    20881 tccagcgtca actgaacttc atccagcgtg cgggaaagat tttcggcagc tatatcgccg
    20941 aattcaaaac caggtaaggc tgacacagct tcacgcagtg ggcgcaggaa aagttgccct
    21001 tgcaaagtat taccattagc aggtgcacca tacaggtagt accccaccac ggagaatttc
    21061 acttcatctt tcggcgttag cggggttttc tcaccggtca gattcagcgc catgcgctct
    21121 ggcataaaat cttcgacgtg gaaatcccac atccgatact ggttatcgcc cgtgttagcg
    21181 cgaatatgcc acataccggt tgccgcattg ctatcgagtg gccaggtaaa gtggtagagg
    21241 ccattctccg gctgactaac gacgctcctg agtacctgcc catcgggttt aatcacgtct
    21301 aacttgatgg gttgattggg caacgcttta ccgtctgcat cacgcagcaa accattgagg
    21361 attaccgttt cacttgggcg ataaagatcg cgtgggccaa acatgaaaaa ctgtttgcta
    21421 tagcctggcg cgccagcaat gttaaattct gctaagtcca gcgccggaag ttttaaatcg
    21481 agtagcgttg tctgaccgtc tttacgcgcc aacagtaatg ccgcgttttt atcattttcc
    21541 agctgcacat gcccctgagc gtcactggtt gcctgagtca gagtctgccc tttctcattt
    21601 aataaagaga cttcaattcc ttgctgggcc gcgccgtttt ccagactttg ggtaaagata
    21661 tccagacgat tgtgataacg gtgagctgaa acgccgatat cacttaacgt aaacagcgtc
    21721 gcgggattac tgtaatcgta acgtccagcc tgattcatca cagccagata cacgcccgcc
    21781 tgctgaagcg gtttgatatc gcccagcggc agcaataatt tttcacgggt gttacgcgca
    21841 ggattgagat caaaccgtcc ggtgtagacc agatccgcca tctgcagcag tttgtctgac
    21901 tgccagttcg ccagcgaatt gcggtattcc cattggctaa tgaatgctgg cagagattct
    21961 ggcttaacgc ggaagaagtt aacatcgaca ttattaacgt tgagcgccat taccggcagc
    22021 ccttcaacga ctttgccagg cagcagcgaa ccacggctgg caaaaccgac gctgggttgg
    22081 atgtcgcggg tagttatagt tttttcgtaa tctttactga aggttgcgtt gttgagtgct
    22141 ttgacctcct tgccaatagt aacgatcaaa tcacgtttcg gttcgaggtg gcgtaaacgt
    22201 aactctttaa gattatctga cagctcccag gcaccatcca ctttgccgct ttttttatcg
    22261 acgacatgaa taacgcgtga gaaatcctga tccgggtcga gagggatgga gaacgtcagc
    22321 accagcgtgg ctgcaccatc aagttggact tcagagagat ccagcaatgt cagcacctta
    22381 ccggcactct gttgggccag tttttgtctc tccggcacgg agagttttgc tgaactcgcg
    22441 ttttctgaag aggccgcttt agtaacttca gaaggcgcat cttttttcac cgctgttggc
    22501 gcgttatcgt tgttgtcgca ccctgccagc gccagcatta gcatgcaggc ggctacgcgt
    22561 aactttttca tttttcatcc ctgccacaat ggcccgttag caacgtcgaa taattattat
    22621 gcgtgagaat ttacgttatg taattcagta tcacgcccat acaatccgaa ttttagtgag
    22681 aattgtggct cagacagcca aatcagcctc tttcgcttgt cgcttttcac taaacatccg
    22741 acaatttagc ctaaccccgg caaaaatgga gatgcctatg tccacgacat ggtttgtagg
    22801 agccgactgg ctcgccgaac atattgatga cccggaaatt cagattattg atgcccgcat
    22861 ggcgtcgcct ggacaggaag atcgtaacgt tgctcaggag tatctgaatg gacatattcc
    22921 cggcgcagtg ttttttgata tcgaagcgct ttctgatcac acttccccgc ttccgcacat
    22981 gctgccgcgc ccggaaacgt tcgccgtggc gatgcgtgaa ttaggcgtta accaggataa
    23041 gcacctgatt gtctatgacg aaggtaatct tttctcagcc ccacgagcat ggtggatgct
    23101 gcgcaccttt ggtgtagaga aagtgtcgat tctggggggt ggacttgcag gctggcagcg
    23161 cgatgatctg ctgttagaag aaggtgcagt agagctgccg gaaggagagt ttaacgccgc
    23221 gtttaatcct gaagccgtgg tgaaagtaac cgatgtatta ttggcaagcc atgaaaatac
    23281 ggcgcaaatt attgatgccc gcccggctgc acgttttaac gcagaagttg atgaacctcg
    23341 cccaggttta cgtcgcggac atattcccgg agcactgaat gttccgtgga cggaactggt
    23401 gcgcgaaggc gaactaaaaa cgaccgatga actggatgcg atattttttg gtcgcggcgt
    23461 cagctacgac aaaccaatta tcgtcagctg cggctctggt gtaacggcag ccgtggtttt
    23521 gttagcactc gcgacgctgg atgtgccaaa cgtgaaactg tacgacggcg catggagtga
    23581 atggggcgcg cgggcagatt taccggttga gccagtgaaa taagtatttt acaggcaata
    23641 aaaaaccgcc gaatttggcg gttttttatt gctggtctgg ttcgcggcct ttccagcagg
    23701 ttgtattacc gtagtaatgc aagcgcgtct cagcggagac aatactcgcc agtaactctc
    23761 tttttgtcaa gcaaaagaga gttattattg ttctgttagt gtattatcca ctgcggccct
    23821 ttccgccgtc tcgcaagcgg gcgctggctt aaggaaagga tgttccgcga ccgttagagc
    23881 aagcgcgtct cagcgcttgc tatcgcggca atatcgccag tggtgctgtc gtgatgcggt
    23941 cttcgcatgg accgcacaat gaagatacgg tgcttctgta tcgtacttat tgtttctggt
    24001 gcgctgtttt cccaagtaaa taataaccgg agtctctccg gcgacaattt actggtggtt
    24061 aacaaccttc agagcagcaa gtaagcccga atgccgccct ttagggcggc atattttaga
    24121 ttatccggtt ctgtttaaag tcacgcaaaa aaccacccca gcgacgttca tagaatggcg
    24181 caatatgttc ggtaataaag tggctaattc ctttttcccc ttttttcacc tgacaaatat
    24241 cgattggttc atcgccaggt aatgtatcgg tcgctacact tcccgtcgcc tgaataattt
    24301 cttcgatatc accatcggct tcaatgccaa taagtaaatt aggctgtgcc tcttcgttct
    24361 ctttaattga acaaataaaa gcacgcttca ccggcttaat ggttttaaat aaggtggtga
    24421 gtgaatcaat catttgtgct ggcggctctg cgacttccga taatatcagc gattcaccgc
    24481 cttccaggat ttcctggctg ctcagcggat ttccctctcc accaatcaac aaactgattt
    24541 cacgcggcat aaattcttta ccggtcggca gtttggcatt aaggaagagc gtttcgccaa
    24601 gggtcatctc aaacaatgtg cgaacgggca ttacgacaaa tgcctgttcg tcttcaaccg
    24661 cctgttgaag cgcttctaac gaggtgaaaa aaggaatgac gctggtgccg tcttcttttt
    24721 cccagtgctg taaatcaagc gcactatctt caaccacagc ctcgccctgc gccgccgtac
    24781 caggcaccca gacggtggat tccagtagag tacggaaaaa gcccgggcgg tgcgccggtt
    24841 cggttgctgc tttttccagc aggtcttcaa gttcgttttt tgtttcggac atatgaacca
    24901 caattcattc aacgttcggg gcgcaaatgc ctgatgcgct acgcttatca ggcctacaag
    24961 ggattcgcaa tttgttgaat ttgcagaatt tgtaggccgg ataaggcgtt tacaccgcat
    25021 ccggcatatt agtttacgcc gttaacagat tagctatcgt gcgcacacca agtcccgtag
    25081 cgcccgcaga ccactgttca accggcgctt tacggtaagt cgccgagcag tcgatatgca
    25141 gccagccttg ctgatagttc tcaacaaagt gcgacaggaa gcccgccgcc gtgctcgcgc
    25201 ctgccggata cgccgcgctt ccggtattgt tcagttcggc aaagttagac ggcagctggc
    25261 tgcggtggaa ttccgccagc ggcagacgcc agaatggttc gttctcttgt gaggcactcg
    25321 ccagcaaacg accggcaagc gcatcgtcaa aactgaacag cgcgtgataa tcattaccca
    25381 gcgcagtttt cgccgccccg gtgagggtcg ccgcatcaat gatcatttcc ggtttctgcg
    25441 cactggcatc aatcagacca tcggcaagca ccagacgccc ttcggcatca gtgttcatca
    25501 cttcaacttt tttaccgttg cgataggtga tgatatcgcc cagcttgaac gcattgccgc
    25561 taatcaggtt atccgcacag cagaggaaca gcttcacgcg cttgttcagt ccgcgcgtaa
    25621 tggcaaatgc cagcgccccg gtaaccgttg ccgcgccgcc catgtccgac ttcatcgagt
    25681 ccataaacgc agtctgtttg atgctgtagc cgccggagtc aaaagtgata cctttaccta
    25741 ccaggcacgc gtacaccggt gcttctttat cgccagttgg gttgtaatcc agcgccagca
    25801 ataccggaga acgttctgaa ccgcgcccga cggtgtgcag ccccatataa ccttgctcac
    25861 gcagatcttc gcctttggtg atccgataag tcacacgatc gcccgcgacg ttgctgatca
    25921 gatcaacagc acgctgtgcc agttgcgatg gtcccaattc ttctgccggt gcgttgatgg
    25981 tgtcacgcac ccagtcgatg atcatcaggc ggttatccag ttcctggcgc tgggcatcgt
    26041 ccagatccgg ccactccact ttacgcgtgc ctttcggggc tttgtaacct tgccagaatg
    26101 cccagcagcg atccgcatcc cagccttcac cgcttaactg aacatgcttg atgcccagac
    26161 cgtcaatctt gcgcgccgca cgctggatca gccccagatc gtctgccccg ttcaaatgca
    26221 gggtaatgcc gtcattatta atgctgtaag ttgctttttc tccccagcgc gcgtcggcag
    26281 gttgggtaga gagggtaatc ttcatcgctt ctgtcatttt agttatcctt ctttgtaaaa
    26341 gggccgccag ttggcagccc caggattttt ccgctaaatg attcgcgttg caggaaggcg
    26401 gcaagtgagt gaagccccag gagcatagat aactatgtga ctggggtgaa cgagcgcagc
    26461 caacgcatct gcggcgtgaa gcatgacgcg gaaattattc ggcctcgtcc agccagacta
    26521 acaaaatcgc ttcgaggatt ttctcgttgg atgcctgcgg gtcgtcgtcg aaatcttcca
    26581 gatcgcaaat ccactgatgc atatcggtga atcgaaccgt tttcggatca agatcgggat
    26641 acgcatcgta cagtgcttcg ccaatttcgc ggctatcggt ccatttaagt cccatactaa
    26701 cctctgttaa tgctcacgcg catggttgat agtgtaacgc gggatttcga ccactaaatc
    26761 ttcgtcggtg acgcgcgcct ggcagcttaa acggctttcc ggctccagtc cccaggcttt
    26821 gtccagcatg tcgtcttcct gctctgagct ttccggcagc gagtcaaaac cttcacgaac
    26881 gatgcagtgg caggtggtgc aagcacagga tttttcacag gcgtgttcaa tctcgatacc
    26941 gttacgcaga gctgcgtcga gaatggtttc accgctatta gcttccagaa cagcgccatc
    27001 agggcagaga tcctgatgag gcaaaataac aatctttggc atattaaacc tcgtccacgg
    27061 aatggccttt cagcgcacga cgaaccgact ggtccatgcg gcgagcggcg aaatcctggg
    27121 tttgtttgtc tacgttttta atcgcttgtt cgatggcgtc aacgtcatcg ccctgcgcca
    27181 cttcactcag gtgagcggca gcatcgtcaa tgacctgacg ttctgcggcg cttaacagcg
    27241 cggcatcagc agccagcgcg ccgtgcagac tttccagcac acgcgccgct tctacttttt
    27301 gttctgccag cattcgggct tttacgtcct gctcggcata gctcattgag tctttgatca
    27361 tcgaagcgat ttcgctatcg gtcagaccgt aagacggttt gacctgaata gacgcctcaa
    27421 cgccggtgga tttctccatc gccgtcacgc tcaaaagacc gtcggcatcg acctggaacg
    27481 tcacgcgaat atgcgcaccg ccagccggta gcgccggaat accacgcagc gcaaaacgcg
    27541 ccagtgagcg gcagtcctgc accagttcgc gctcaccctg cattacatgg atagacatcg
    27601 ccgtctgacc atctttaaag gtggtgaaat cctgagcgcg ggccaccgga atagtggtat
    27661 tacgcggaat cactttctcc accaggccgc ccatcgtttc gaggcccagc gacagtggga
    27721 tcacatcaag caacagcatt tcgctgtctg gcttgttacc caccagaata tccgcctgaa
    27781 tcgccgcgcc aatagcgacg actttatccg ggtcgatgga agtcagcggt ggacgaccga
    27841 aaaattcgcc tacccgttca cgcaccagcg gcacgcgagt agaaccgccc accatcacca
    27901 cttccagcac ttcgtcagct tctacacccg cgtctttaag cgcgcgacga caagccagta
    27961 aggttcgttt taccagtggc gcgatcagtt cattgaattg ttcacggctg atttcgccct
    28021 gccagcccgc aacgttaacg gtcacggagt ccgcatcgct cagcgcgatt ttggctgcaa
    28081 tggcggcatc cagcagttca cgctgaacgc ggttatcgct acgatcagga atgcccgcct
    28141 gctcgcgaat gtaatccgcc agcagatggt cgaaatcatc accaccgagc gcggaatcac
    28201 cgccggtcgc cagcacttca aacacaccgc gacttaagcg cagaatggaa atatcaaacg
    28261 tcccgccgcc gaggtcataa acggcgatca cgccttcctg accggaatcc agcccgtagg
    28321 cgatagccgc agcggtcggt tcgttaagta agcgcaggac gtgaaggccc gccagacgcg
    28381 ccgcgtcttt ggtgccctga cgctgggcat cgtcaaagta cgccggaacg gtgataacta
    28441 caccatccag ctcgcctgcc agggcttcag ttgcccgcgc cgccagtgct ttgaggatgt
    28501 ccgcagaaac gcgcaccggg ttcagcagcc ccgccgccgt ttcaatcatc ggcaggccgt
    28561 tttcgctggc ctggaattga taaggcagat gcggatagcg ttgctggata tcagccagcg
    28621 agcgccccat caggcgttta acagaactaa tggtgttggc ggtgtcaagc gctgcgttag
    28681 tacgcgcgtc ataacccacc gaatgccctt gctgttgata gtgaacaaca gatggcagca
    28741 ggtgacggcc ttcatggtcg gctaacgttt cggcctgacc gctgcgcact gtcgccacca
    28801 gcgagttggt tgtgcccagg tcaataccag ccgccagacg acgctgatgc ggcgcagcac
    28861 tcaaaccagg ttcactaatt tgtaataagg ccatgtttag cttccagaaa ttaaaaatcg
    28921 agcagttttt cttcgagttg ttcggcactg ctccgcagtt tatcgagaaa acgcagctta
    28981 cgcacggtat ccgccgccgc gtcccacgcc tcgttgtcta actgttcaac catcaactga
    29041 tggcgggtat caaacatctt tttcacacgt ttgataaagc tttccagccg cgcttcatct
    29101 ttcgcctgtt cgatctcgtc cagctcttcg cgcagctcca actgttccat caggaacgcg
    29161 gtgtcgcgca cagtatgctg ctcgctggcg agatcaaaac cgtgtaaaga aagcaaatat
    29221 tccgcgcgca ttaacggatg acgcagcgtt tgccaggcct ggttaatggt tgcagattgc
    29281 tgtacggcgg cgagttgttc cgcctggctt ccgctggcga atttatcagg atgatactga
    29341 cgttgtagat cctgaaaacg caggctcagc gcctgggtat cgagttgata acgggcaggc
    29401 aagccaaaga gggtgaagta atccataaca ttctcagggc tgcgatccgc cgcgataacg
    29461 gcgaatcagg taaaacaaac cccacgcgca ggcgaccacg gtggggttat cggtatgcgc
    29521 atcaaacgtg gaagctttcg ccgcaaccac actcatcttt aacgttcggg ttggtgaatt
    29581 taaacccttc attcaggcct tcttttacga agtccagctg cgtaccgtcc agaaattgca
    29641 ggcttttgcc atcgaccacg actttcacgc ctttgtcttc aaacacgatg tcttccggcg
    29701 tcggttcgtc aacaaattcc agtacataag ccatacctga acatccggag gttctcacgc
    29761 ccagacgcag gccaaaccct ttgccgcggt tagccagaaa ggtatttact cgcgctgctg
    29821 cactgtcgct cagtgtaatc gacataacca aacctcaact cttattttgc ttcacgtttg
    29881 cttttatagt ccgcaatggc ggctttgatc gcgtcttctg ccagaataga acagtgaatt
    29941 ttcaccggcg gcagttcaag ttcttcagca atatcggtat ttttgatcgc ctgcgcttcg
    30001 tcgagagact tccctttcac ccattcggtg accagggagc tggaagcgat agcagaaccg
    30061 cagccgtaag ttttaaaacg cgcgtcttca atgatacctt catcgttgac tttaatctgc
    30121 aacttcatca cgtcgccaca ggccggtgca cccaccatgc cgctgccgac gttctcgtcg
    30181 ttgttgtcaa aggaacccac gttacgcgga ttctcgtaat ggtcgataac tttttcgctg
    30241 taagccatta taaattctcc tgattccgat accgattaat gatgagccca ttcgatgctg
    30301 ttcagatcca cgccctgctt gtacatttcc cacagcggag aaaggtcacg cagacgaccg
    30361 atggatttac gaactaactc gatggtgtag tcgatctctt cttcagtagt aaaacgacct
    30421 aaagagaaac ggatagagct atgtgccagc tcgtcgttca gccccagcgc gcgcagcacg
    30481 taggacggtt cgaggcttgc tgacgtacag gcggaacctg aagaaactgc gaggtctttc
    30541 agcgccataa tcagcgactc accttcaacg tagttgaagc tgacgttgag aatgttcggc
    30601 gcaccgtgtt ccaggtcacc gttcaggtaa acttcttcga tatctttgat gccgttccac
    30661 agacggttac gcaggccgcg cagacgttcc atctcggtcg ccatctcttc ttttgcgatg
    30721 cgataggcct cgcccattcc gacgatctgg tgaacaggca gagtgccgga acgcataccg
    30781 cgctcgtgac cgccgccgtg catttgcgct tcgatgcgta cgcgcggttt acgacgtaca
    30841 tacagcgcac cgataccttt cgggccatag attttgtgac cggagaaaga catcaggtca
    30901 actttcaact ggctcaggtc gataggcagt ttgcccacgc tctgggttgc atcaacgtga
    30961 tagataatgc cacgagcacg gcacatttcg ccgatagccg cgatatcctg caccacgccg
    31021 atttcgttat ttacgtgcat gatggaaaca aggatggtgt cgtcacgcat cgctgcttca
    31081 agttctttca ggtcgataat gccgttacgc tgcggtgcca ggtaggtgac ttcaaaacct
    31141 tcgcgctcca gctgacggca ggtatccagt accgctttgt gttcggtttt gctggtgatg
    31201 atgtgcttgc cttttttctg ataaaagttg gctgcacctt tgatcgccag gttgtcagat
    31261 tcggttgcac cagaggtaaa gacgatttca cgcggatcag cgccgaccag atcggcaatc
    31321 tgattacggg cgatatctac cgcttcttca gcctgccagc cgaaacggtg agaacgagag
    31381 gccgggttac caaaggttcc gtccatcgtc ataaactgca tcattttctc ggcaacacgc
    31441 gggtccaccg gcgtggttgc ggagtagtcg agataaatcg gtaatttcat tgctctataa
    31501 actccgtaca tcactcaatg caaggaacca ggctaccggc tggatgtacg accgtgttta
    31561 cggagcgcga tacgccccgg cctgattcta atatcttgtt atctttaacc taactgtttc
    31621 gggtttcagc aagttgacaa acttacgaat ctccgggagc ttacataagt aagtgaccgg
    31681 agtgagtatg tgcagtcaac gcagatgcaa cacgaaacag gacggttaaa ttaagcgcgt
    31741 aacttaacgt cgatcgcgtc ttgtatgcgg gtgcgtggcg cgtcgtgagt atgctgacga
    31801 ccagacacat ccagcacttc ctggttatta accagttcgc ctaaagtaat gttgttgaga
    31861 aaaccggtga gacggtcgct caaatcacgc cacagcgcgt gggtcaggca tttatcgccg
    31921 ccctggcagc cgcctttacc ctgacaacgg gtggcatcta cagattcgtc aacggcgcta
    31981 atcacttcgc caacggcgat gctgctggca tctttgccta acagataacc accgcctggt
    32041 ccacgtacgc tggaaaccag accattttta cgcagacggg aaaacagttg ttccagataa
    32101 gaaagggaaa ttccctgacg ttcggaaata tcagccaacg gtaccgggcc cgcttcagag
    32161 ttgagcgcaa cgtcaagcat tgcggtcacg gcatagcgcc ctttagatgt cagtctcatg
    32221 tcttacttca cctcaaactc gcccctgccc ggggtttttt attgtaaaag tgggggtatt
    32281 gcatagcagg gtcaagtctg acattcccga gtaaattggt caactattta cttgactgat
    32341 ttagtcgggt atttaacctt cagtgccatt tttttatcgt ggcgtttgta cctgttgtcc
    32401 ttccagacca tgggcgggtt gagggcaatt attcgcatta gcgctggcct cttcccctca
    32461 ccctaaccct ctccccgaag gggcgagggg accgtccgtg catttatctg gcttagccct
    32521 caggccttat tacctttatt ctgctgctca atagaagcca gaatcccgcg caggatattc
    32581 aattcctggc tttccgggcg cgcacgggta aacagacggc gcaatttatt catcacctgc
    32641 cccggatggt tttcacggat aaaaccggtt gccagcagcg tttgttccag atgaccataa
    32701 aaacgctcca gatcatcgac cagcggatac ggcgtctctt catgctcgac ctgctcgccg
    32761 ttttcctgag ttgccagcca ggccatgcgc acttcatagg caataacctg agccgccatc
    32821 gccaggttca gcgagctgta ttccgggtta gccgcaatcg cgacatgata atggcatttc
    32881 tgcaactctt cattggtcaa gccgacgcgc tcgcgaccaa acaccagcgc caccggggta
    32941 tttgccgctt cagcgacgct tttcaggccg cattcgcgcg ggtcgagcat cggccatggc
    33001 agcgtgcggg agcgtgcgct ggtgcccacc accaggctac aaccggctaa cgcttcgtcg
    33061 agcgtatcga cgatgtgagc attaccaatc acatcgctgg cccctgctgc cagggcaatc
    33121 gcctgggagt cgggtttcac cagtggatta accagccaca gattggttaa tcccattgtt
    33181 ttcatggcac gggcaacaga acccatattg ccggtgtgtg acgtctccac cagcacaatt
    33241 cgaatatttt gcagcattgt ctttcttcat ctaaagatta ttcacgcatc ttatcataaa
    33301 acgaagacag atgccgatct cgctgctata ctctgcgccg ttttcccgtt ctttaacatc
    33361 cagtgagaga gaccgatgca tccgatgctg aacatcgccg tgcgcgcagc gcgcaaggcg
    33421 ggtaatttaa ttgccaaaaa ctatgaaacc ccggacgctg tagaagcgag ccagaaaggc
    33481 agtaacgatt tcgtgaccaa cgtagataaa gctgccgaag cggtgattat cgacacgatt
    33541 cgtaaatctt acccacagca caccatcatc accgaagaaa gcggtgaact tgaaggtact
    33601 gatcaggatg ttcaatgggt tatcgatcca ctggatggta ctaccaactt tatcaaacgt
    33661 ctgccgcact tcgcggtatc tatcgccgtt cgtatcaaag gccgcaccga agttgctgtg
    33721 gtatacgatc ctatgcgtaa cgaactgttc accgccactc gcggtcaggg cgcacagctg
    33781 aacggctacc gtctgcgcgg tagcaccgct cgcgatctcg acggtaccat tctggcgacc
    33841 ggcttcccgt tcaaagcaaa acagtacgcc actacctaca tcaacatcgt cggcaaactg
    33901 ttcaacgaat gtgcagactt ccgtcgtacc ggttctgcgg cgctggatct ggcttacgtc
    33961 gctgcgggtc gtgttgacgg tttctttgaa atcggtctgc gtccgtggga tttcgcggca
    34021 ggcgagctgc tggttcgtga agcgggcggc atcgtcagcg acttcaccgg tggtcataac
    34081 tatatgctga ccggtaacat cgttgctggt aacccacgtg ttgtgaaagc catgctggcg
    34141 aacatgcgtg acgagttaag cgacgctctg aagcgttaat gactcaggcg ggtgatgtca
    34201 ctcacccgcc ctcgcctttc aggcgctatt ctgaaatact tcctcaccgc tttactttct
    34261 ttcgtcactc tcccaccatc tttccccgat taatggatga taaagaagtc gcaaggaaag
    34321 gttatggcac tgccagtgaa caaacgcgtt cccaaaattc tgtttattct ctttgttgtt
    34381 gccttctgcg tttatttagt gccgcgcgtt gccatcaact tcttctatta tcctgacgat
    34441 aaaatttacg gtcccgaccc ctggtcggcg gaatccgtcg aatttacggc taaggacggt
    34501 actcgtctgc aaggctggtt tattccttct tcgacgggcc ctgctgacaa cgccatcgca
    34561 accatcattc atgctcacgg caatgccgga aatatgtccg cccactggcc gctggtcagt
    34621 tggttacccg agcgtaattt caacgttttt atgtttgatt atcgcgggtt tggtaaatca
    34681 aaaggcacgc cgtcccaggc cggattgctg gacgatacgc aaagcgccat caatgtggtg
    34741 cgccatcgca gtgatgtaaa cccacaacgg ctggtgctgt tcgggcagag tattggcggg
    34801 gcgaatattc ttgctgttat tggtcagggt gatcgtgaag gcatacgtgc ggtgatcctc
    34861 gactccacat ttgcctctta tgcaaccatc gccaaccaga tgatccccgg cagtggctac
    34921 ttacttgatg agagttacag cggcgaaaat tatatcgcca gcgtcagccc gatcccgctt
    34981 ttactcattc acggtaaagc tgatcacgtt atcccatggc agcacagcga aaagttgtat
    35041 agcctggcaa aagagccaaa acggttgatc ctaatcccgg atggcgaaca cattgatgct
    35101 ttttccgatc gccacggcga tgtttatcgc gaacagatgg tggactttat ccttagcgcg
    35161 ttgaatccgc agaactaacc catgatcgct agcacgataa tcattcacaa aaccacctta
    35221 agacatgcta atccactggt cagaacagtt taagatgaga aaaattctgt gacgcttgcc
    35281 aacatttctg atgattagca ttcccttcgc catttccctg agcaaacttt agctattctt
    35341 atcaattatg cttatgggag atctacagat gatgcctacg cttgctccac catctgtcct
    35401 ttcggctccc cagcgccgct gccagatctt gctgacgctc tttcagccgg ggttgaccgc
    35461 caccatggca accttcagcg agcttaatgg tgtggatgat gatattgcca gtcttgatat
    35521 cagcgaaaca ggacgggaga tcctgcgcta tcatcaactc acactgacga ctggttatga
    35581 cggtagctac cgggttgaag gtacagtgct taaccaacgt ttgtgtttat ttcactggct
    35641 acgacgtggt ttccgtctgt gtccgtcatt tattaccagc caattcaccc ccgccctgaa
    35701 gagtgaactg aagcggcgcg gaattgcgcg taacttttac gacgatacca atctacaagc
    35761 gttagtgaat ctctgctccc gacggctgca aaaacgcttt gaatcgcgcg atattcattt
    35821 cctgtgtctg tatctgcaat attgtttgct gcagcaccac gctggaatta cgcctcagtt
    35881 taatccgctc caacgtcgct gggcggaatc ctgccttgaa tttcaggtag cgcaggaaat
    35941 tggacgccac tggcagcgtc gggcgctcca gcctgtaccg cctgatgagc cactgtttat
    36001 ggcactactt ttttccatgt tgcgggttcc cgatccactg cgggatgcgc atcagcgaga
    36061 cagacaattg cgtcagtcta tcaaacgtct ggtaaaccat tttcgtgagc tgggaaatgt
    36121 tcgtttttat gatgaacagg ggttatgtga tcagctttat acccacctcg cccaggcgtt
    36181 aaatcgcagt ttgtttgcca tcggtattga taataccctg ccggaagagt tcgccagact
    36241 gtacccacgc ctggtgcgca ccacccgcgc ggcgctggcc ggatttgaaa gtgaatacgg
    36301 cgtccatctt tctgatgagg aaagtggtct ggtcgcggtg attttcggtg cctggctaat
    36361 gcaggaaaac gatctgcatg aaaaacagat tattctactg accgggaatg atagcgagcg
    36421 agaagcgcag attgagcagc agttacgcga actaacgtta ctgccgctca acattaagca
    36481 tatgtcggta aaggcatttt tgcagacagg cgctccgcgt ggcgcggcac tgattattgc
    36541 gccttatacc atgccattac cgctcttttc accaccgctg atctatacgg acctgacgct
    36601 gacaacacat caacaggagc agatccgcaa aatgctcgaa tcagcatgag ggaacaactt
    36661 tcgggcgcaa aaacattgcc ggcagcgcca ccagcgccat tacccagaac acgccgtggc
    36721 ccagatattg atacaggaaa ccggcgaaaa cggtcatgat agcgatactg ccgcccatcg
    36781 cgacggcaga gtaaaccgcc tgtaaacgga tgacttcgct accctggcga gcagcaatat
    36841 aacgcatggc ggccaggtgg cagaccgtga aggtgccgca atgcagaatt tgcaccacta
    36901 tcaaccacgg caacgccgta gttgctccca taatgcccca gcgcactacg ccgcaaatcg
    36961 ccgagatcaa cagcatatcg cgtgcactac aacggcggaa aagtttatta ctcagcgcaa
    37021 agataatgac ttccgccacc acgcccagcg accacaaata ccccaccgcc gaggccgagt
    37081 agccagctgc ctgccagtaa atggcgctaa aaccgtaata ggccgcatgt gccccctgca
    37141 ataaacaaac gcaggccaga aagcgccagt tctggcgaac cagcgccaac cacgcagacc
    37201 aaccggtgct ctcctgctgg cgacttgccc cttgtggctg aatcgtcgga cggatgagaa
    37261 agccgagcag catggatgcc acgcccaacg tcaacagcgc gaggatcacc cgataatcaa
    37321 acatagtgac cagtttgccc gtcagcgccg agccaatgac aaacgccacc gagccccaca
    37381 gtcgcacttt gccgtaatca agcgggaact gcttttgcca cgtattcgcc agtgcatcgg
    37441 tcaacggtac cagtggtgag aaaaagaggt taaagccaat catcaccagc atcagccacg
    37501 ctacgtgcgc ccccgcccag aaggcgacag caaagagaag tgtcagcagt gccagcacgc
    37561 gcaaggcgga aatcaggcgg gaaggatcgc tgacgcgggg cgcgatgagc aaactcccga
    37621 ggaaacgggc aaccagacct gcccccaata acaggccgat ggtttctggc gttaaaccaa
    37681 tccctttaag ccagacgctc cagaaaggta gaaaaatgcc gtaactaaaa aagtatgtga
    37741 aatagccgag cgccaaccag cgcgtggatt gcaaaaccat gagtccctcc cgtcaggagg
    37801 cgttagtctg gggttaatcg aatgttggtg caagttgaaa aataagaata ttcgagcctg
    37861 cttcccgatg tctgtaacgc cagttcacaa gcactaaaaa actgagatcc gcgacgcatg
    37921 ttgcaaaaat aatgcgtaat tatgccgtta cgcttgccaa acgttcctgc acaatggcga
    37981 tgaaatcgcg taatgccggc ttcatctccc ctttcttcca cgccatcagt aaagcaatgg
    38041 aaggcacatt tccggcaatg ggacgaaaaa caacctgtcc ggtattaaaa ttattcatat
    38101 aaccgggtat caaagtgacg cccagcccca tgcccaccag attcatggtc accagaatat
    38161 tcgttgccac ctggacgata tttggctggc tgttttcttg cgcaaaccac gctttaacga
    38221 tcggcgcaag cgaaccggaa tacaccggat cggtactgac gaaattcacg ccatccagtt
    38281 gcgcggcggt gatctctttt tcatgtgcta aagggtgatc aaccggtaac acaaccacta
    38341 atggttcgtc aaaaagctcc agataatcaa tctccgggct ataaacagga tggcgcatta
    38401 agccgacgtc gagttcacca cggcgaattt tttcctcctg ttgcgtggtg attaaactca
    38461 ccagctcaat caaggtgtct ggctgtctga gacgaaacat cggtaatact tttggcagta
    38521 aattcacttc cgccgatggc acaaagccaa tggttaattg tctgtcttcc tgaacaattt
    38581 tccgcgcccg taatttggca ttttccgctt gttcgaggat tgccagcgcg tcctggagaa
    38641 aacattctcc cgccgccgtc aacgcgactt tgcgcttatc cctcaccagt aacggaacac
    38701 cgacacagtt ttcaagatcg cggatctggc tgcttaacga aggctgtgag gtatgcagtt
    38761 tttccgccgc acgggtaaag ttcagtgcct gcgccactgc gacgaaatag cgtaaatgcc
    38821 gtagttccat caccttcccc ttgttatcga aaaaacgtct cagctggtag aaattaacta
    38881 tttcacatat tagcaaccaa ccagcaacat ccttatggca caaaaataga aggtcaatac
    38941 atcttatctt tcaggattaa aaaatatgac cacaccctca gatttgaaca tttaccaact
    39001 gattgatacc caaaatggtc gggtcactcc gcgtatttat accgacccag atatttacca
    39061 actggagctt gaacgtattt tcggccgttg ctggttattt ctcgcccacg aaagccagat
    39121 cccaaaacct ggtgatttct ttaacaccta catgggggaa gatgcggttg tcgtagtgcg
    39181 tcagaaagac ggcagcatta aggcgtttct caaccaatgc cgccaccggg ccatgcgtgt
    39241 gagttatgca gattgcggca acactcgcgc ctttacctgt ccgtatcacg gctggtctta
    39301 tggcattaac ggcgagttga tcgatgtacc gctggaacct cgcgcctacc cacaagggtt
    39361 gtgtaaatcc cactggggac taaacgaagt tccttgtgtg gagagttata aagggctgat
    39421 ttttggcaac tgggatacca gcgcaccggg cctgcgtgat tacctgggtg acattgcctg
    39481 gtatctggat ggcatgctgg atcgtcgcga aggcggcacc gaaattgtcg gcggcgtaca
    39541 aaagtgggtg atcaactgta actggaaatt cccggcagag cagttcgcca gtgaccagta
    39601 tcatgctctg ttcagccatg cttctgccgt tcaggtatta ggggcgaaag atgatggcag
    39661 cgataagcgc ctcggtgatg gacaaaccgc ccgcccggtg tgggaaaccg ccaaagatgc
    39721 gctgcaattt ggtcaggacg gtcacggtag cggtttcttc tttactgaaa aaccggatgc
    39781 taatgtctgg gtcgatggcg cagtttcaag ctattaccgc gaaacctatg ccgaagcaga
    39841 acaacgttta ggtgaagttc gcgccctgcg cctggcgggt cataacaata tattccccac
    39901 gctttcatgg ctaaacggca ctgccacact ccgcgtctgg catccgcgcg gccctgatca
    39961 agttgaagtg tgggcgttct gtattactga caaagccgcc tccgatgaag ttaaagccgc
    40021 ttttgaaaac agcgccactc gtgcttttgg tcctgctggt tttctcgagc aggatgactc
    40081 ggagaactgg tgtgaaatcc agaaattgct taaaggccac cgcgcccgca acagcaaact
    40141 gtgtctggaa atggggcttg gtcaggaaaa gcgccgcgac gacggcattc ctggcattac
    40201 taactatatc ttttcagaaa ctgccgctcg tggaatgtac caacgctggg ctgatcttct
    40261 gagtagcgaa agctggcagg aagtgctcga taaaaccgcc gcttaccagc aggaggtgat
    40321 gaaatgagtg cgcaagtttc actagagtta catcaccgca ttagccagtt tctctttcac
    40381 gaagccagct tactggacga ctggaaattt cgtgactggc tggcgcagct cgacgaagag
    40441 attcgttaca ccatgcgcac cacagttaac gcgcaaacac gcgaccgccg caaaggcgtc
    40501 cagccaccga caacctggat ttttaatgac accaaagacc agctggagcg gcgaatcgcc
    40561 cgtctggaaa cgggcatggc ctgggcagaa gagccgccgt cacgcacccg tcacttaatc
    40621 agcaactgcc agataagcga aaccgacatc ccaaacgtat ttgctgtgcg ggtaaattat
    40681 ctgctttatc gggcacaaaa agagcgcgat gaaacattct atgttggaac gcgtttcgac
    40741 aaagttcgcc gtctggaaga tgacaactgg cgcttgctgg aacgggatat cgtcctggat
    40801 caagcggtaa tcacttccca taacctgagt gtactgttct gatgaatcga atttatgcgt
    40861 gtcccgttgc ggatgtgccg gagggtgagg ctctccggat cgatacctcg cccgtcatcg
    40921 ccctgttcaa cgttggcggc gagttttatg ccattaacga tcgttgcagc catggtaatg
    40981 cgtcaatgtc agaagggtat ctggaagatg acgccacggt ggagtgcccg ctacacgccg
    41041 ccagtttttg cctgaaaacg gggaaagcgt tatgcctgcc cgccaccgat ccgctcacca
    41101 cttatccagt acacgttgaa ggtggtgaca ttttcatcga cttaccggag gcgcagccat
    41161 gagcgatctg cataacgagt ccatttttat taccggcggc ggatcgggat tagggctggc
    41221 gctggtcgag cgatttatcg aagaaggcgc gcaggttgcc acgctggaac tgtcggcggc
    41281 aaaagtcgcc agtctgcgtc agcgatttgg cgaacatatt ctggcggtgg aaggtaacgt
    41341 gacctgttat gccgattatc aacgcgcggt cgatcagatc ctgactcgtt ccggcaagct
    41401 ggattgtttt atcggcaatg caggcatctg ggatcacaat gcctcactgg ttaatactcc
    41461 cgcagagacg ctcgaaaccg gcttccacga gctgtttaac gtcaatgttc tcggttacct
    41521 gctgggcgca aaagcctgcg ctccggcgtt aatcgccagt gaaggcagca tgattttcac
    41581 actgtcaaat gccgcctggt atcctggcgg cggtggcccg ctgtacaccg ccagtaaaca
    41641 tgccgcaacc ggacttattc gccaactggc ttatgaactg gcaccgaaag tgcgggtgaa
    41701 tggcgtcggc ccgtgtggta tggccagcga cctgcgcggc ccacaggcgc tcgggcaaag
    41761 tgaaacctcg ataatgcagt ctctgacgcc ggagaaaatt gccgccattt taccgctgca
    41821 atttttcccg caaccggcgg attttacggg gccgtatgtg atgttggcat cgcggcgcaa
    41881 taatcgcgca ttaagcggtg tgatgatcaa cgctgatgcg ggtttagcga ttcgcggcat
    41941 tcgccacgta gcggctgggc tggatcttta aggaagcacg atgaaagaaa aaaccatcat
    42001 tattgtcggt ggcgggcaag cggcggcaat ggctgcggcc tcgctacgcc agcaagggtt
    42061 caccggtgag ctgcatctgt tttccgatga gcgacatctt ccttatgaac gacctccact
    42121 ctcgaaatcc atgttgctgg aagattcccc gcagttacag caggtgttac ccgctaactg
    42181 gtggcaggaa aacaatgttc atctgcattc cggtgtaacc atcaaaacgc tgggtcgcga
    42241 cacacgagag ttagtgttaa ccaacggcga aagctggcac tgggatcagc tttttatagc
    42301 aaccggcgcg gcagctcgac cgctgccgtt gcttgatgca ctgggagaac gctgctttac
    42361 cctacgccat gccggtgatg ccgccagact gcgagaagtt ctgcagcccg aacggtcagt
    42421 cgtgattgtc ggtgccggaa ctattggtct ggaactggct gccagcgcca cgcagcgcag
    42481 atgtaaggtg acagtgattg aactggcggc aaccgtcatg ggccgtaatg caccaccgcc
    42541 cgtgcaacgc tatcttttac agcgccacca gcaggctggt gtgcgcattc tgctcaataa
    42601 tgccattgaa catgtggtcg atggtgaaaa agtagaactg acgctgcaaa gtggggaaac
    42661 gcttcaggct gatgtggtga tttacggtat tggtatcagc gccaacgagc aactggctcg
    42721 cgaggccaac cttgatactg ccaatggcat tgtcattgat gaggcttgcc gcacctgcga
    42781 tcccgcgatc tttgccggtg gcgatgtggc aatcactcgt cttgataatg gtgcactaca
    42841 ccgctgcgaa agctgggaaa acgccaataa ccaggcgcaa attgccgctg ccgcaatgtt
    42901 agggctaccg ctaccgctac tgccgccgcc gtggttctgg agcgatcagt acagtgataa
    42961 cttacagttt attggcgata tgcgtggcga tgactggctt tgtcgtggca acccggaaac
    43021 tcagaaggcg atttggttta atctgcaaaa cggcgtgctt atcggtgcgg tcacgctgaa
    43081 tcaggggcgt gagattcgcc caattcgcaa atggatccag agcggcaaaa cgtttgatgc
    43141 gaaactgctg atagatgaga acatcgcgct taaatcactg taaccaggat aattagcgaa
    43201 tatctcaatg cctggggcgt ggcgaggtgc aagagtgtgt attacgttta aatcacatta
    43261 tcttgcaaag ggattggtta tgaacacact acgttatttt gattttggag ctgcccgccc
    43321 cgttttgtta ttaattgccc gtatcgccgt ggtcttaatt ttcattattt ttggttttcc
    43381 caaaatgatg ggctttgacg gtacggtcca atatatggcc tcgttgggcg cgccaatgcc
    43441 gatgctggca gcgattattg cggtagttat ggaagtgccc gccgcgatat taatcgtgct
    43501 tggctttttc acccgtccgc tggcggtgct gtttattttc tacacgctgg gtacggcggt
    43561 gattggtcac cattactggg atatgaccgg cgatgcggtt gggccaaata tgattaattt
    43621 ctggaagaat gtcagtatcg ctggcgcatt cctgctgctg gcaattaccg ggccgggggc
    43681 aatttctctc gatcggcgtt aggaaagatg ccggatgcgg cgtgaacgcc ttatccggca
    43741 tttaataaat tacagccact ctacccgcaa cgacatctct gaagttgttg attcccctgg
    43801 tgctagcgca atgagatcac ccccttccgg gcgatgatga tcatccggcg catggctcat
    43861 cggttccaga cagaagaaat caaacgcata tcctttatca aacgcagggt cggaaacaaa
    43921 gatgaaataa cacggtgcag gtggcgtcgt ttccatgatg atggcataac cttcctgcgg
    43981 ctgctcgatg cgggcctgac cattccatcc ggcaaaacca ttgttcaccc actggcgcgg
    44041 caacggcgcg ggctggttaa aatccagttc ctgcggtagc tgctcgcaaa actcacccgc
    44101 cagccactgc tcccgctcca gccagtaacc gctcgcctgt gcctgaatcc gcgtttgcgg
    44161 cgacaacgga aaataaggat gccagccggt accaaatggc agcgtctctg ccccttgatt
    44221 ggtgacagag agcgtcaccg tcagcgtatc cgccgttaaa tgaaacgcct gacttacccg
    44281 atagtgatag acaccgctgc gatgttcata caccagacac aaactatcat cgctgtgcga
    44341 gacacattgc cactcgccca gccagccatc gccgtgtaga tagtgcgcat cccactcaac
    44401 attcggttgc agttgatact cacgcccctg ccagacaaaa cgattgccgc tcacccggtt
    44461 tgcaaatggc accagcggaa aacatgaggc atcggttgcc acaccgcttt ttttaccagg
    44521 acgtaagagc ggcgtcgtat cgcgccagaa gccttcgatt acgccgccct ggtcagaaac
    44581 gtccagcttt agcgacccgt gggataaggt atagatggtc atccgctaac tccttaatcc
    44641 gggaagttaa tcacaacttt cccgcatttg ccgctcgcca tcagcgcata ggcatcacct
    44701 gcctgttcca gcgagaagcg atgggtaatg gcgttacgcg gccacagttt ccagtccgtc
    44761 agatcatggg cgcatttttc catatggaac agactggtca cccaggagcc gataatccgc
    44821 cgttgatggt gcatcagatc ggcgctgacc tcgaactcca cttttccggt ttctccgatg
    44881 taaaccaccc gtccccagtc agcggtggat cgcagtgcca gcaagcgacc tgcggcatta
    44941 ccagaacaat cgagcgcaac atccgcgcca ccgtgggtga gttcggcgat aatctgcggc
    45001 agaccttcgg tggttgctaa atagccgtga tccatcaccc ctaactgttt tgccatcgcc
    45061 agacgttccg gcagcatatc aacgccgatg atccgttttg caccgcgacc tttcgccagc
    45121 atcatcgcca tcatgccgac tggccccaga ccgaccacca gcacgttatc actgccggaa
    45181 acttcgccgc gcaaaattcc ttcatacgct gtaccaacgc cgcaactgat aaacgcacca
    45241 tcttcgtagc tcagcgcatc cggcaggagg atcagatctt tttcttccgc cagcagatat
    45301 tcggcatgac cgccgtcacg ctgccagccg taagccgctt ttcctttgcc agtacaagag
    45361 ataggaaagc cgcggcggca gttcgggcaa aaaccacagc cagaaatgtg atacaccagc
    45421 acgcggtcgc cctctttaaa atggcggcag ccttgcccca tcgccacaat ctgcccgcac
    45481 ggttcatgac cgttgataaa tccctggtat aacggtttat cgggtgccgc cgccgtggca
    45541 cggtgttgat gatagatata gtggacatcg cttccgcaaa tcccggagga tttcattttg
    45601 atcagtacct ggttaatccc cggcgtcggc accgcaactt cccgcagatc gacggttgaa
    45661 tttcctggta aataagctgc cagcatcgtt ttcataaatc cctcattaac aatacgatta
    45721 attttcatcc ctgccgcacc cgcgccaggg gcgttaaggt ttagcgtttc gctttactgc
    45781 ttcgctgggt cagcaagata ttcgccagca ccgccaccac gatgatgacg ccgcgtacca
    45841 cctgctggaa aaaggagtta ataccgagca gcaccagacc gttaccgatt agcgtaatca
    45901 ccagcacacc aagcaatgta ccgaacaggg aaccgcgacc gccggaaagt gccgtaccgc
    45961 cgaccacgac cgcggcgatg acgtcaaact ccagaccgtt tgcggcacct gcgttaccgg
    46021 aaccgaggcg tgccgccagc aaaatgccgg tcaccgccgc taataatccc gaaagggtaa
    46081 agataagaat gcgcactcga cgaacgttaa taccgcacaa ctgcgccgcc gtggcattac
    46141 cgccaacggc aaaaaccgag cgcccgaagg cggtttttcg gctaatgaac acaaacagcg
    46201 caaacaacac catcatgatc agcgcggata ctggcacacc gagaaattgt ccgcccagcc
    46261 agtccagcac ctcgttttcg tcaatcggca ctggcagtgc gttggtcata aacagcccca
    46321 ttccgcgcag ggcgctccac agccccagtg tggcaacgaa acttggtacg ttaaacacgc
    46381 cgcgcagcac cccggcgagc gttcccatca gcgcgcctaa cagcaacacc agcagacacg
    46441 ccaccgccag cggaacttca aattgcagca aaaatgccag gcacaccgag acaaaagcca
    46501 ccatcggccc aacgctgaca tcaatttcac cggagataat aatcagcgtc atcgcccagg
    46561 cggcaatccc aatggtggcg gcatcgcgca gcacgttcat ctggttattc aatgagataa
    46621 agcctggcgc gttcagggag aagaccagat aaagaatggc aatcaccacc agcaaaccga
    46681 tctcattaat atggcgactg acaaattgtt tgagcgagac gctcttgccc tgcggcaatg
    46741 gtaatgacga agcagacatc ttcaggttcc tcggtaaaat tcatcagtgc acagacagaa
    46801 tggcggacat cagctcatcc acattgaccg gagcgtgaaa ctcctgcgag aacgtgccgt
    46861 gctgtagtaa caggatgcgg tcacagacca gcggcaactc ctccacttca ctggagataa
    46921 acaccacgct ttttccttcg gcagccagct cacggacaat acggtaaatc tgctgtttgg
    46981 cttcgatatc gacgccgcgc gttggctcgt cgagcaacaa aatctggctg gcggcataga
    47041 cccaacgacc gatcaccact ttttgctgat tgccgccaga aagcgtgccg atgggcgttt
    47101 cgctactggc ggccttgacc gtcatccgct gcatcacctc ttcggtcagg cggcggatgg
    47161 tggaccattg cagtacgccg ttggcgctga ttttttgccg attggtcagc actgtatttt
    47221 cgtcaacgcc caaccaggga atgatccccg cttctttgcg gttttctggc gtatagccaa
    47281 tgccgcgttt cagcatgtcg ccgtaatcgg ggcgcgtgat tttctcgccg ttgataacaa
    47341 tttcgccctg ttcatactcc tccagcccaa caatcgcctt cagcaattca ctgcgccctg
    47401 cccccagcag gccagcaatg ccaagcactt cgccgcgacg taacgtaaag ctgatatcct
    47461 ccagcttggg cttatggcgt aatgcacgga cttccagcac ggcctgatcc acaatttcct
    47521 gaggggcaac cggcgcaata tcaacgtgat cacgccctag cattagcgac acaatatgat
    47581 gcgtggaggt gttttcgagc atcacatcgc ccgccacctg accatcgcgc ataacggtgg
    47641 cacaggaggc aatgcggcga atttcttcca tccggtggct gacataaatc accgccacgc
    47701 ccagtgccga catctttttc accgcgatga tcaccagttc aacttccgca ctcgcaagcg
    47761 aactggtagg ttcatcaaga atgaccacgc gcggctcgcc cttcatcacc cgcgcaattt
    47821 ccaccagttg cttttgcgcc gggcttagcg ttgaaacaag ttgttcaggg ctaacgtcaa
    47881 cgcccagcgc ctgtaagcaa cgttgggcat cctgcgccat ttgcaggtaa tcaatcatgc
    47941 cgttgcggcg gggccactga ccgaggcaga ggttttccgc caccgtcagt ccctccacca
    48001 gacttaactc ctgataaacc gcacgaaccc ccagttcagc ggcacggcga gtcaacgtag
    48061 cttcgtcacc ttccagtcgc gtctcgccaa tccagatatc accgctatcc gggcgttcgc
    48121 tgccggtaag cattcgaatg agagtcgatt tgcccgcgcc gtttttacct aacagcgcac
    48181 gaacttcgcc tttattgagc gtgaagttaa cgttatccag cgcaacgacg ccggggtaac
    48241 gcttatttcc tgccaccact tttgctaccg ggactgcctc tgttgccgtg aacataggga
    48301 cctctgcgaa tcagcgatta gggcagacca tcaacgtgcg ttgccagcca ctgtttacca
    48361 tcttccgttt tggtatagag atcgattggc acctgaatca ctttttcacc gtcggcttgt
    48421 ttattgataa ccttcaatgt ttgcgcgaaa acagcattgc ccattttctt accggaaata
    48481 tccactaccg ctttcagcac ctgattgttt tccagctcct gagcaatttc ggttgtcata
    48541 tccgaaccga aaacagcaat ttttccggcc tgattttgat tacgtaccgc ttttaccgcg
    48601 ccgagtgtcg caccgcccga ctcccccata atggcgttga gatccggcgt ggagataatc
    48661 agtttttcac caacggaaat cgctttatct aaaacagtcc cttcctgatt agcgacaatt
    48721 tgcgcgccgg gaacgcggga ttttaatact tcttcaaatc ctttacgtcg ctgcacacaa
    48781 acttcaaagg cttcgcaatt gatgacagca attttcggct ggtcaatttt attggcaata
    48841 aaataatcgg cggcagcgtt acccagtttt ttaccaaatt ccagcggatc gccgaccaga
    48901 tacgccgaga catatttatc gccccccttt tgattaatac aggtgttgta gcaaatcacc
    48961 ggaatacccg cttcactggc gcgacgaacg gtacggctac tgccattttc agacactgcc
    49021 gataaaataa tggcatcgac attacgcgcc acgagggtat caacaaaggt actttctttc
    49081 gaaatatcac cctgggcgtt agtttcaatt aactgtacct gtactgaaga atcttttgcc
    49141 gcatcctgaa cgccctgacg cactccagcg taatatccct gggtatcaag gtatattgca
    49201 ccaatggtca tttctttatc cgcagccctg gcaaatagtg cgctacctaa tagcgtagcc
    49261 attaataata gattacgggt tgttctcatt tttttaggca tagtgcctcc tgtagggttt
    49321 ttattaacaa cggcttattc taattatttt gtgatgagcg gcagcgcgtg ccgctcaaat
    49381 attacagtta atagggttta gtgaacgtag ttaaaaataa aaggcatcac ggtggtgaat
    49441 aatgccgctt tcggccaggc cggattaatc gccgtcaatt cagcgcgagc agattcatat
    49501 tgcgcaacct ccccgagtcc cgcggacgcc agcatcgcca ccatcaggca tttttcttta
    49561 tgctgctgtt gtaaatcgcc atacagcgac aacaggtcag gttgtgaaac agcaaagaaa
    49621 tccgcctcaa tactggtttt cgccatctct tgcgcccact gtttcatttc actaaacagt
    49681 tgctgtgcgg tttgctgttc acccagcagt cgcagcgcca ttccttgcca gaagagataa
    49741 tcaaccggct gatcgttgta ataactgtgg atgttaatgg tgcgatcgcc ggtcgccgcc
    49801 agacgtaaac aacgcatcgc ttcagtttca tcgccctgcg cgttggcgca tatcgcctgc
    49861 cagaaccaga tgtcgttatc agtttgcccc ggtaaacggc cttcgcttaa attctccgga
    49921 taatgcagcg cggcatgaag cagttcgctg gcctgctgcg gctctctggc atcaagatgc
    49981 tgccaggcgc gtaataactg gttgaggata aactgactgg tgaccttccc ttccccgcct
    50041 tcccacgggt ggaatttacg cgtggcgaga atgtccgccg ctttgtctgc ctgacccgtg
    50101 agatgccaca aattgagcag ttctgcggtc atgtcgtcgc gtttcagcgc aatttcctga
    50161 ttattttcca gacgcgccag tcgtttctcc ggtgtggctc cacttaactt atcaagcaaa
    50221 tcccgttcga aaagcagacg tgcatcctgc ggcgcaagct gataagcatt atcaagataa
    50281 cgcgcggcca gctcataatc gtgttgctta ttccacgcat ggatcgctaa cccgcgccag
    50341 ccgtcggcaa actccggcga catctctacg caacgttgcc ataaggcaat ggctttgttg
    50401 tagctacgtt tgttgtagta gaagcaggcc agtaaatggc gagcaaacca gcactcttca
    50461 atactctcca gcgccgccac ttcttccagc gtattcggga aacggacaaa ctgcgggaag
    50521 acatcaatgg cttttgcgac cagttcgcca cgttcggctt tcggcagcaa gctggcttgc
    50581 aggtaaagcg gcagcgtgcg ctggcagtcc agtgcgttca gcatctctgc cgccagggtg
    50641 ggcattcccc agttaatcag ttgcccggcg gtcatcaggg cgttaacgtc gcgtccctga
    50701 cacagaccgc gccactgcgc gagagctgat tcgctacgac catcgaacca gttcagccac
    50761 cacagagtgg cgttcagcgg ataatcgcgc agcagtttct cgcgctgcac acgcgcgttg
    50821 tcctgacgac cacttaacac cagcagcaga ttatgcaggc aaagcacttc ctgattggtt
    50881 gggcaggcgc gaagactttg ttggcaaaaa tccagaccag cgtcgaagtt accattacgc
    50941 gccgccagtc gtgccaggcc ataatagcca ccggctttgc tgttgccgct ccagaccgca
    51001 cgccagaaat cctcttcggc ttgttgatat tgtccctgac gttcgtaagc actggcgcga
    51061 atcaaactcg cctgtccgca ctgcggattt ttgttcagcg catgtgcgcg tttcagagcc
    51121 tgagtggcat acgccaccgc ttgcgggaaa tcggcgcggt tatattccag catcgccagc
    51181 gccaggttac agcggtaatc cagcggatcc agcgccacgc cgcgcaggta gtaatcgaac
    51241 ggtgaacggc tggcgtgatg atattgctcc agatgctgac cgataaacca ggcttcatct
    51301 gtactggtaa tgtcttgtgc tgccagtggc gctttggcga cgtccggcaa cggcaactct
    51361 tgcggctgat gttcctgata actcagtaca atattgccgt cggcatcgga gagctcaatg
    51421 gtcagccttt caggattgat accgtgcaac acgccctgga tggcggtcgc aggcatcagt
    51481 gccacggcat catcaagtaa cgcgttgcat ttgccgattt cgcggatcgc caggcggtat
    51541 ccgttcaacg gagagatggc atacagcccc cactcaatcc cccgcttact acgctggagt
    51601 tttatcaccg catcgcggga ggcattttgc accatgccca aagaatgata aggcaggaaa
    51661 tactgctcaa aacgcttctc ttcgtaagca tcaagccagg taaaatcggg ctggttatcg
    51721 gcaaaaatac cggtcatcag ttcgatatac ggaccgttat tgtcggtcag actcttatcc
    51781 cacgcctggc caaattcact gtgtccccaa ctccactgtt ttttacctgg cgcaatatgg
    51841 tggttggcaa cgtgcagcaa accgccatct tcatcgtgac accacgcgcc gacaaaatcg
    51901 tactgcgatt tttcggccat atatgaggtt ggaacaggca catttttata gcgagaaatg
    51961 tccactccgg cggagtagtc cactttgtag taagtgccgg tggcgatggg gaaagcggag
    52021 acggcccgtt tgccgtgatc aaacaccgca gtcacatccg gtgggaaaac gctctgatgc
    52081 ccttcccccc ctttcactgc cgggttggcc caccacaaga aatgacgcgg cgtggcgtta
    52141 ccgttataga cgcggctggc gatttccagc gccgcccggt cagggcgcag ggtgaaacct
    52201 gtcatcacct gtaaaccatg catcggctcc gtttcgccta cccacaccgt ctgtgcaccg
    52261 tcttcatggg cttcgagggt gaaatcaacg ggcataaagg tggtcgggcg atgatgttgc
    52321 ggccagttaa actcaatccc accggaaatc cacggcccca gcagccccac cagcgcaggt
    52381 ttaatgactt cattgtgata aacaaaatcg cgctgtttca ctttatccca cgcgcgatgc
    52441 acccgaccgc ccagttccgg caggatcatc actttgatgt agtcgttttc cagccacacc
    52501 gcctgccagg atttcagggt tttctgctcg ctcagcgtat cggtcacgcc gtagggataa
    52561 accgcgccgg acgatccctg ataaacgcga ttttcgagga acatgggatg tatatcctgc
    52621 ggcccggttt cataggtcgg gatctcaacg cgctcttgcc acacttttac tggagtcatg
    52681 gtgccctcaa tattaacaag acatactgaa ttaaaagatt tgtggcagtg tattgaacaa
    52741 tctggcaatg ttttcgcgga ataatcacgc aattaactaa acaaggttta gtgaagatga
    52801 gagcctgcat taacaatcaa cagattcgcc accataacaa atgcgtgatt ctggaactgc
    52861 tgtaccggca aaagcgcgcc aataaatcaa cgctggcccg gctggcgcaa atttcgattc
    52921 cggcagtcag taatattttg caggaactgg aaagcgaaaa acgggtggtg aatattgacg
    52981 atgaaagcca gacgcgcggg catagtagcg gtacatggct gattgcgccg gaaggtgact
    53041 ggacgctgtg cctgaacgtg acgcccacca gtattgagtg tcaggttgct aatgcttgtt
    53101 taagtccgaa aggtgaattt gagtatttac agattgatgc accgacgccg caggcgctgc
    53161 tgtccgaaat cgaaaaatgc tggcatcgcc accgtaaatt gtggccggac cgcaccatca
    53221 atctggcgct ggcaatccac ggtcaggttg atcctgtgac cggcgtgtcg caaaccatgc
    53281 cgcaagcgcc gtggacaacg ccggtggagg tgaaatatct gctggaagag aagctcggca
    53341 ttcgggtgat ggtcgataat gactgcgtga tgctggcgct ggcggagaaa tggcaaaata
    53401 attcgcagga acgggatttc tgcgtgatca acgttgatta cggcattggc tcgtcgttcg
    53461 tgattaacga gcaaatttat cgcggcagtt tgtatggtag cggacagatt ggtcacacca
    53521 tcgttaatcc ggatggcgtc gtctgcgact gtggacgtta tggctgcctg gaaaccgtcg
    53581 cctcgttaag cgcattaaaa aaacaggcgc gggtatggct aaaatcacaa ccggttagta
    53641 ctcaacttga tcctgaaaaa ctgactacag cgcagttaat cgctgcctgg caaagtggag
    53701 aaccgtggat caccagctgg gttgaccgca gtgccaatgc cattggtttg agtctgtata
    53761 acttcctcaa catcctcaat attaatcaga tttggttgta cggtcgcagt tgtgcctttg
    53821 gtgagaactg gcttaatact attattcgcc agacaggatt taacccgttc gaccgcgacg
    53881 aaggaccgag cgtgaaagcg acgcaaattg gcttcgggca attaagccgc gcacaacagg
    53941 tgctgggaat tggctatttg tatgttgagg cgcagttacg gcagatttga tggcgcaata
    54001 acgtagaaag gcttcccgaa ggaagccttt tttcaacaga ttaaccgttt cgcttatgcg
    54061 taaaccggat aacgtgcgca gatgtcgaga actttacctt tgatgcgctc gataacagct
    54121 tcatcattga tgctgtccag cacgtcacac atccagccag ccagttcttt cgcttctgct
    54181 tctttgaagc cgcgacgcgt aattgccgga gtacccacgc gaataccgga ggtcacaaac
    54241 gggctcttcg gatcgttcgg tacgctgttt ttgttgacgg tgatgttagc acggcccaga
    54301 gcggcgtctg cttctttacc ggtcaggttt ttatcaacca gatcaaccag gaacaggtgg
    54361 ttatcagtgc cgccggaaac cactttgtag ccgcgctcga ggaacacttc taccatcgct
    54421 ttagcgtttt tcgcgacctg ctgctggtaa gttttgaact caggctccat cgcttctttc
    54481 agagcaaccg ctttaccggc gattacgtgc atcaacggac cgccctgacc accagggaaa
    54541 acggcagagt tcagtttttt gtacagctct tcgctaccac ctttcgccag gatcaggccg
    54601 ccgcgcggac ccgccagggt tttgtgagtg gtggtagtaa caacgtgagc atgaggaacc
    54661 gggttcgggt agacgccagc agcaaccagg cccgcaacgt gcgccatatc aacgaacagg
    54721 taagcaccga tgctgtcagc gatttcacgc attttcgccc agtccaccac gccggaatat
    54781 gcagagaaac caccgataat cattttcggc ttgtgttctt tggcttgttt ttccagatcg
    54841 gcgtagtcga tatgaccggt agcatcgata ccgtaaggaa cgatgttgta cagtttaccg
    54901 gagaagttaa ccggagaacc gtgagtcagg tgaccgccat gcgccaggtt catacccaga
    54961 acggtatcac ctggttccag cagcgcggtg tagaccgcaa agttagcctg ggagccggag
    55021 tgcggctgga cgttagcgta gtcagcgccg aacagttctt tcgcacgatc gatcgccagt
    55081 tgttcaacga tatcaacata ctcgcaaccg ccgtagtagc gtttgcccgg ataaccttca
    55141 gcatatttgt tggtcagctg agaaccctgc gcctgcatta cgcgcgggct ggtgtagttt
    55201 tcggaggcga tcagttcgat gtgctcttcc tgacgtactt tttcctgctc catagcctgc
    55261 cacagttcgg catcataatc ggcaatgttc atttcacgct ttaacatccg catctcctga
    55321 ctcagctaac aataaaattt ttggccttta taggcggtcc tgttggacaa cggcgaacag
    55381 tataaccgaa tcattgtgcg ataacaggtc ttgacaaagg aatttacgca aacgattacc
    55441 ttcaggctac gcaaggcttt ggagaataaa gagcttgcaa ccggaaacgg atttcttttc
    55501 aggtttgtga tgcaaatttt tcacttcatc acattctttc tgaaaaacac caaagaacca
    55561 tttacattgc agggctattt tttataagat gcatttgaga tacatcaatt aagatgcaaa
    55621 aaaaggaaga ccatatgctt gacgctcaaa ccatcgctac agtaaaagcc accatccctt
    55681 tactggtgga aacggggcca aagttaaccg cccatttcta cgaccgtatg tttactcata
    55741 acccagaact caaagaaatt tttaacatga gtaaccagcg taatggcgat caacgtgaag
    55801 ccctgtttaa cgctattgcc gcctacgcca gtaatattga aaacctgcct gcgctgctgc
    55861 cagcggtaga aaaaatcgcg cagaagcaca ccagcttcca gatcaaaccg gaacagtaca
    55921 acatcgtcgg tgaacacctg ttggcaacgc tggacgaaat gttcagcccg gggcaggaag
    55981 tgctggacgc gtggggtaaa gcctatggtg tactggctaa tgtatttatc aatcgcgagg
    56041 cggaaatcta taacgaaaac gccagcaaag ccggtggttg ggaaggtact cgcgatttcc
    56101 gcattgtggc taaaacaccg cgcagcgcgc ttatcaccag cttcgaactg gagccggtcg
    56161 acggtggcgc agtggcagaa taccgtccgg ggcaatatct cggcgtctgg ctgaagccgg
    56221 aaggtttccc gcatcaggaa attcgtcagt actctttgac tcgcaaaccg gatggcaaag
    56281 gctatcgtat tgcggtgaaa cgcgaagagg gtgggcaggt atccaactgg ttgcacaatc
    56341 acgccaatgt tggcgatgtc gtgaaactgg tcgctccggc aggtgatttc tttatggctg
    56401 tcgcagatga cacaccagtg acgttaatct ctgccggtgt tggtcaaacg ccaatgctgg
    56461 caatgctcga cacgctggca aaagcaggcc acacagcaca agtgaactgg ttccatgcgg
    56521 cagaaaatgg cgatgttcac gcctttgctg atgaagttaa ggaactgggg cagtcactgc
    56581 cgcgctttac cgcgcacacc tggtatcgtc agccgagcga agccgatcgc gctaaaggtc
    56641 agtttgatag cgaaggtctg atggatttga gcaaactgga aggtgcgttc agcgatccga
    56701 caatgcagtt ctatctctgc ggcccggttg gcttcatgca gtttaccgcg aaacagttag
    56761 tggatctggg cgtgaagcag gaaaacattc attacgaatg ctttggcccg cataaggtgc
    56821 tgtaatttga tgttgccgga tggaaacatc cggcaaccct tgacgcggtt taaattgccg
    56881 cgtcgtcctc ttcaccggta cggatgcgaa tgacccgtgc cacgtcgaag acgaagattt
    56941 taccgtcacc gattttgccg gtttgcgccg tgcgaataat ggtatcaaca caggtatcga
    57001 caatgtcgtc cggtacgaca atctcaattt tcactttcgg cagaaaatcc accatatact
    57061 ccgcgccgcg gtacagctcg gtatggcctt tctggcggcc aaaacctttc acctcagtga
    57121 ccgtcatgcc ggtaatgccg acttcagcca gtgcttcgcg aacatcgtcc agcttgaagg
    57181 gtttaataat cgcatcaatc tttttcatgc tattccttga aaaggtcgcc tgtcttttga
    57241 tctgctaaac gtaacacata acgccaattc attccttgaa atcgtttgca tccagctcgt
    57301 gtcgggaaag cagtttataa aattctgtcc ggttgcgccc cgccattctc gccgcgtgtg
    57361 tgacgttgcc tttggtgatt tgcagcagct tacgcaaata gttgagttca aactgattac
    57421 gtgcctcaac aaaggttggc agcgccgtat tttcaccctc cagcgcctgc tctactaatg
    57481 catcgctaat aaccggagat gaggtcagcg ccacgcactg ttcaatcacg ttgaccaact
    57541 ggcgcacatt acccggccag ctagcggtca tcaggcgttt catcgcatca gtggagaacg
    57601 cgcggacaaa cggtttatgt cgctctgccg cctggcgcaa caggtgattt gccagaagcg
    57661 gaatgtcttc tgtgcgctct gccagagccg gaattttcag gctgacaacg ttgaggcggt
    57721 aatagaggtc ttcacggaat tccccgcgcg ccatcgcttt tggcaggtcg cggtgagtgg
    57781 cggaaataat ccgcacgtcg atatcaatat cgcggttact gcccagcggg cgcactttac
    57841 gctcctgcaa cacgcgcagc agtttgacct gcaacggtgc gggcatatcg ccaatctcat
    57901 cgagaaatag cgtaccgcct tccgccgcct ggaataaacc ttcgcgattg ctgacagcgc
    57961 cagtaaacgc gccacgcgca tgaccaaaca gctccgactc cagcaattgc tcgggcaatg
    58021 cgccacagtt aatagcaata aatggtttgc tattgcgcgg gctggcgttg tggatagcct
    58081 gggcgaaaat ctctttcccg gtgccgctct ggccgttaat caaaacgcta acgtctgatt
    58141 gcgccaccag ccgcgcctgt tccagcaaac gcagcatcag cgggctgcgg gtgacaatcg
    58201 cctcgcgcca gcgttcatcg gtggctggcg cggattgttc cagcgcatcg tcaattgcct
    58261 gatatagcgc atctttatcg acaggcttgg tgaggaaact gaacacgccc tgctgtgttg
    58321 cagcaacggc atcgggaata gaaccatgcg cggtaagaat aattactggc attcccggct
    58381 gcactttctg aatttcagca aacagctgca taccgtccat ttcatccatc cgcaggtcgc
    58441 tgatgactaa atctactttt tcgcgattca gtacccgtaa tccttcagcg ccactttccg
    58501 ccgtgaccac gctgtagcct tcgctggtca ggcgcaggcc aagcagtttc agcaatcccg
    58561 gatcgtcatc gaccaataat aaatgcgcag gtttatggct catcaggagt gacctcatgg
    58621 gtggatggcg cgggcttttc accctcgtgc ggcgtatccg gcgagaaatt tccagccggt
    58681 ttgcgggtcg agagctggcg ttcaatatca gtcaggtttt ccagcttgcg ggtggtgagt
    58741 tccagttgct gctgtagaac gtgatgttgc tggcgcaatg tatccagctc gctgtcgctg
    58801 gcctgctgga gcttgccgta gcgttggcgc tcttccgcca gttgcagttg cagcgcctga
    58861 ccgtcgcgcc agagttgata cagagggcga acctgtgccg ggatctcggt acttagcgca
    58921 tcaatgcgcg cgaccagctg gcggcgctca tacggcgtaa ttttggcgtc ggcgagcaaa
    58981 atcccttgtt taaaggtatt ttgccagctg ccgtcgtcat attggcgagc ttgctgacgt
    59041 gactgcgcag gcattaaacg atcagcacaa tccatcgccc gcagccagta aagcggattg
    59101 gtttcggttg atttaccttg cagcgcccag atgtcgctac attcagtaga aagatagtca
    59161 gccagttgat aaaccggaat tttttcttct gctggcgtat caatggctgg cttattgtga
    59221 ctctgcacgc aacccagcaa tgccagacat ggcagccctg ccagccacag ccgtcggggc
    59281 aataatcgtt gaaaaatgtg tcgcatattc accagactta atgcctatcc cagtgggcgt
    59341 aattattgca ggcagtttgg acatggacag cgcggagaaa ccggagcgta cacgtcgtac
    59401 gtgaggattt cgagcactgc ccggggccga actggcaaat aaaatagcct gatgggatag
    59461 gctgttagca ttatttcgtg tttttcgacg acggcaattc aatgcggaaa caaacgtctt
    59521 gcccgctctc gtcgaccaga tacagttccc cttgcatacg gcaaatacaa tccctggcaa
    59581 tgcttaatcc cagaccgctg cccttcaccg ccccttttcg ctggtggctt ccttgaaaaa
    59641 agggttcgaa gatcatggcg cgttcctctt gcggaatggg tgtgcctgta ttgatgacat
    59701 caatataaac ccgtgcacca tgtaagctgc tgcgaaggca aatgttaccg gattcagccc
    59761 cgtagtgcac cgcattggag taaagattat ccagtacgct catcagcagc attggctccg
    59821 ccaggcaagc tgttgctttg agatcgacgt cggtatgcat cattttagcc cgtgcgggca
    59881 ggctatgagc agaaaccact gtctccacca gcggtgctaa ttcaacattc tccagttcca
    59941 ccgcactgtc cgcctgttta cggttgtaat caagcagttg ttcgatcagt ttttgcaaat
    60001 tgcggctgct gctatcaaga atgctcacca cctctttttg ctctggcgta agcggcccga
    60061 caacctggtc agccagtaat tcagtgccct cgcgcatact cgccagtggc gtttttaatt
    60121 catgagataa atgtcttaaa aattgatggc gttgggattc cagccatgac aggcgctcac
    60181 ttaaccaaag aatacgttgc ccaaccgagc gtaactcgct cggtccactg aacgaaacgc
    60241 tattgcccag agaacgccct tcccccagac ggttgatcat gcgctcgata tttttcaccg
    60301 gcccgataat catccgcgtg aaaagtagca ccatcaccag actcaccaga aatagcacca
    60361 gcgattgcca accaaaatat tgcccacgtt cggcgatttc acgctgaagt tgctgcccac
    60421 gagagaacac cactgtgcgc gtggcctgca ccatttcggt attggcactg gcaaaggctt
    60481 caagacgcgc ggcggcggca gcatcgggac cactgttgtt acactgaagt tgggccagat
    60541 tgttcaaatc ctgacgcaat gcctggtaaa gtttatcgtc tggcagtacg cctgcgtggg
    60601 catcgagcat ttcgctgtaa cgcttgcgct ggctttgata aaccttcgca agcgtagggt
    60661 cgtccagcac gcaatactga cggtagctac gctccatctc cagcgccgcg ttggtcatcg
    60721 cttcactacg ccgggcatca ataagcgtag tgcggttaac cagcgccgcc tgatcgctaa
    60781 gcgcattcag gctttgccag gcttgccatg ccagcaccaa caggggtagt agaattagca
    60841 aaaatgccag cattaccagt tgtcgtaatg agcggggaaa aacgggccag cgtttcaagg
    60901 tgttactctc gtcagacgcg aatagcctga tgctaaccga ggggaagttc agatacaaca
    60961 aagccgggaa ttacccggct ttgttatgga ataaggcggt gcctaactcg acgtttcgcc
    61021 cgatggttga tatagctacg ctgatatcag aagttggacg gcaggcacct tgttgtgcgt
    61081 cattcgaatt ttatgtagca catcccgaag gggctgacat aagtcggtga atgagccact
    61141 gcttactatt atgcagtaac tgtgccaata aagaaaatag tttggtaacg tactgattat
    61201 acgttgcttt gagggattta tggcttcttc gctatttgaa tgtaaatcag ccactgtgtc
    61261 gctaaaaaga gacaacttaa gataaactta ttagattaca ataaaatcaa tcatttaagt
    61321 gtcgccagaa agcgacacgg taaaccaccc attgtcgtga tttacagaca caaaaaagct
    61381 cccggagttg ggagcttatg atagtggttg gtgcttaatg ccggatgcgg cgtaaatgcc
    61441 gcatccgggc tgcaaaacca atgaactgat gacttacccc aactgcttac gcgcattgcg
    61501 gaaaatgcgc atccatgggc catcctcgcc ccagttttcc ggatgccagg agttgctgac
    61561 agtacggaaa acacgttccg ggtgcggcat cataatggtg actcgaccac tttcagtcgt
    61621 aaccgacgta ataccgttcg gagaaccgtt cgggttagcc gggtaggttt cagtgacttt
    61681 gccgaagtta tcgacatagc gcagtgccac cagccctttg ctttccagtg ccgccagatg
    61741 cgccgcatca cgcacttcca cgcgcccttc accgtgagag acagcaatcg gcatttgcga
    61801 gcccaccatc ccctgcaaca gcagcgacgg gctttgggtc acttcaacca ggctgaaacg
    61861 cgcttcaaag cgatcggagg tattgcgcac aaaacgtggc cacaactcac tacctgggat
    61921 cagttcacgc agattagaca tcatctggca accgttacat acccccagcg ccagcgtttg
    61981 cggacggtgg aagaaggttg caaactcatc gcgtacacgg tcattgaaca ggattgactt
    62041 cgcccaacct tcaccggcac ccagcacatc accgtaggag aaaccaccgc acgcgaccag
    62101 ggcgtggaaa tcttccaggc ccgtgcgtcc agccagcagg tcactcatat gcacgtcgat
    62161 agcatcaaag cctgcacggt ggaaagctgc cgccatttca acatgcgagt tcacgccctg
    62221 ctcacgcagt acggcaactt tcgggcgcgc gccagtggca ataaacggtg ctgccacatc
    62281 ttcgttgata tcgaacgaca gttttacatt caggcccgga tcggcgtcgt ttgatttcgc
    62341 ctggtgctcc tggtcagcac actccgggtt gtcacgcagg cgctgcatct gccaggtagt
    62401 ttctgcccac cagacacgca acgtagtgcg gctttcgctg aatacagtct gcccgttggc
    62461 ggtaatcaca aaacggtcac cggaaaccgc ctgcccaaca taatggacgc aatcagcaag
    62521 cccatgctgt gccagtacgg actcgaccgc ttcacggtca gcggcacgaa cctgaatcac
    62581 cgcacccagt tcttcgttaa acaacgccgc caggcgatcg tcacccagag agccgatatc
    62641 cgcatcaatg ccacaatgac cagcaaaggc catttccgcc agtgtgacca acagaccgcc
    62701 atcagagcgg tcgtgatacg ccagcagctt acgctgtgca accagcgcct gaatcgcgtc
    62761 atagaagcct ttcagttgcg cgacatcgcg tacatctgcc ggtttgtcgc caagctgacg
    62821 ataaacctgc gccagcgccg ttgcgcccag cgcgttattg cctttgccca aatcaatcag
    62881 cagcagtgcg ttatcttcgg tagaaagctg cggcgtgatg gtgtgacgta catcttccac
    62941 gcgggcaaat gcagaaatca ccagcgacag cggcgacgtc atttcgcgct cttcgttacc
    63001 ttcctgccag cgggttttca tcgacataga gtctttaccc accgggatcg tcaggcccag
    63061 cgccggacaa agctcttcgc ccacggcttt aacggcttca tacaggcccg catcttcacc
    63121 agggtggcct gccgccgcca tccagttggc ggaaagttta atgcgtttga tatcgccaat
    63181 ctgtgttgcg gcaatattgg ttaacgcttc accgaccgcc agacgggcag aggcggcgaa
    63241 atccagcagc gcaaccggcg cacgctcgcc aatcgccatc gcttcaccgt agtagctgtc
    63301 gaggctggcg gtagtgaccg cgcagttagc gaccggcacc tgccacggac ccaccatctg
    63361 atcgcgcgct accatgccag ttacgctgcg gtcaccgatg gtcaccagga aagttttctc
    63421 cgccacggtc ggcagatgca gtacgcgttt caccgcgtct gcaatggtga tcccttcacg
    63481 gaccagcgca tcgcccttcg ctttcagcgt ttgtacatcg cgggtcatct tcggcgtttt
    63541 accaagcagg acgtccagcg gcagatcgat cggctgatta tcaaaatgac gatcgtgcag
    63601 agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg tagggtgcgc gctcacgctt
    63661 acacagttcg tcaaacagcg gtaattgatc ggcagcaacc gccagcacgt agcgttcctg
    63721 ggattcgtta caccagattt ccagcgggct catgcccggt tcgtcgctta gaatatcgcg
    63781 cagttcaaat ttaccgccgc gtccgccgtc gctcaccagc tccggcatcg cgttagaaag
    63841 accgcccgca ccgacgtcgt ggataaacag aattgggtta gcatcaccaa gctgccagca
    63901 acggtcgatc acttcctggc aacgacgctc catctccggg ttgtcgcgct gtacggaagc
    63961 aaagtcgagg tcggcatcag actgacctga cgccatggaa gacgccgctc cgccgccaag
    64021 accgatgttc attgccgggc cgccgagaac gaccagcttc gcaccgacgt tgatctcgcc
    64081 tttttgtacg tgatcggcgc gaatgttgcc gatcccgccc gccagcatga tcggtttgtg
    64141 ataaccgcgc agctcttcgc cgttgtggct gttcactttt tcttcataag tacggaagta
    64201 gccattcagt gccggacgac caaattcgtt gttaaacgcc gcgccgccca gcgggccttc
    64261 ggtcatgatg tccagcgcgg tgacaatgcg ctcaggctta ccgaaatctt cttcccacgg
    64321 ctgttcgaag ccaggaattc gcaggttgga aacggagaaa ccaaccaaac ccgctttcgg
    64381 cttcgcgccg aggccggtgg caccttcatc gcggatttca ccgccggaac cggtcgccgc
    64441 gcccggccac ggagaaatcg ccgtcgggtg gttgtgagtt tcgactttca tcagaatatg
    64501 cgccggttcc tgatggaaat cgtagcggcc cgtttcgtgg tcagcaaagt agcggcccac
    64561 ttcagaacct tccattacgg cggcgttatc tttataagca gagagaacgt aatctggcgt
    64621 ggtttcgaag gtgtttttga tcatcttaaa cagcgatttc ggctgttgct caccgtcgat
    64681 aacccaatcg gcgttaaaaa tcttgtggcg gcagtgctcg gagttcgcct gggcgaacat
    64741 atacagctcg atgtcgttcg ggttacgacc aagctttgtg aaagcatcct gcagatagtc
    64801 aatttcatct tccgccagtg ccaggccaag acgcaggtta gcgtcgatta gcgcctgacg
    64861 gccctgcccc agcaaatcaa cgctggtgac cggagtcggt tgatggtgtg caaacagctg
    64921 ctctgcatca tctaaagcaa aaaagaccgt ttccatcatg cggtcgtgca gttcagcggt
    64981 aacctgctgc cattgttcat tggtcagcgt accggcttct atatagtaag caacgccgcg
    65041 ctcaaggcgg tttacctgtt gtagcccgca gttatgggca atatcggtcg ctttcgaaga
    65101 ccagggagag atggtgccag gacgcggggt caccagcagg agtttgcctt gcggggcgtg
    65161 gctggcgagt gccgggccat atttcagcag gcgttcaagt tgtgcgtgct catcatcgtt
    65221 taacggcgca ttgaggtcag caaaatggac atactcggcg taaatattgt gaaccgggag
    65281 cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg aatgccgaca gtgcaggcga
    65341 accacgcaga atttccatca taagtctctc gtcttctaag ctttcggtgt acccaagggg
    65401 gggaaacggg cgtcattata aagaatctga tgcgctgacg aaaccgtttg cgtggaaata
    65461 aaatcaccat cgtgaattag caacgcgtgc cgccaatggc tgtaataagt tgccatctgg
    65521 cgcaggttta cgcaaaatgc cgctcattta agagtaaacc tttcactatt attacgtttt
    65581 ttcaagctgg gacgcgcacg acacagagaa ttaactaatt gaaaaaatta aagattaatt
    65641 atctgttcat cggcattctg gcactgctgc tcgcggtcgc tctctggcca tccattccct
    65701 ggtttggtaa agccgacaac cgtatcgccg ccattcaagc gcggggagag ttgcgtgtga
    65761 gcaccattca tactcccctg acttataacg aaatcaacgg gaaacctttt ggcctggatt
    65821 acgaactggc gaaacagttt gccgattacc tcggcgtaaa actgaaagtg accgtgcggc
    65881 agaatatcag ccagctgttt gacgaccttg ataatggtaa cgccgacctg ctggcggcag
    65941 gactggtcta taacagtgag cgggtaaaaa attatcagcc tggccctacc tattattccg
    66001 tgtcacaaca actggtttat aaagtgggtc agtatcgccc acgtacgctg ggcaacctga
    66061 cggcggagca actcaccgtt gcaccgggtc atgtggtggt taacgatctc cagaccctga
    66121 aagaaacaaa attcccggaa ttaagctgga aggtagacga caaaaaaggc tctgcggaat
    66181 taatggaaga tgtcatcgaa ggaaaactcg attacaccat tgctgattct gtcgccatca
    66241 gcctgtttca gcgcgttcac ccggagctcg ccgtagcgct cgatatcacc gatgaacaac
    66301 cggtgacttg gtttagcccg ttagatggcg ataataccct ttccgccgcc ctgctcgact
    66361 tcttcaacga aatgaatgaa gacggtacgc tggcacgcat tgaagagaaa tacctggggc
    66421 atggcgatga ttttgattac gtcgatacgc gcacattttt acgcgccgtc gatgcggtac
    66481 tgccgcagtt aaagcccctg tttgagaaat acgccgaaga aattgactgg cgtttgctgg
    66541 ccgctattgc ttatcaggaa tcgcactggg atgcacaggc cacttcaccg acgggtgtgc
    66601 gcggcatgat gatgttaacc aaaaataccg cgcaaagcct cggcattacg gatcgtaccg
    66661 atgccgaaca gagcatcagc ggtggcgtgc gttatttgca ggatatgatg agtaaagtgc
    66721 cggaaagtgt gccggagaac gagcggatct ggtttgccct cgctgcgtac aatatgggct
    66781 atgcgcatat gctggatgcc cgcgccctga cggcaaaaac caaagggaat cctgacagtt
    66841 gggctgacgt aaaacagcgt ctgcctttac ttagccagaa accctattac agcaagctga
    66901 cttacggcta cgctcgtgga catgaagcct acgcttatgt cgaaaatatt cgtaagtatc
    66961 agattagcct ggtgggttat ctgcaagaga aagagaagca ggctacagaa gcggcgatgc
    67021 aactggcgca ggattatccg gcggtatcgc ctacggagtt gggcaaagag aaatttcctt
    67081 ttctctcgtt tctttcccag tcgtcatcaa actatttgac ccattctccc tctctgctgt
    67141 tttccaggaa agggagtgaa gagaaacaaa attaatccgt cgaggattgc gcttttttct
    67201 gcgctttaat ttcctggcgg cgcatgcgaa agaagtcact gagcaacgcc gcgcactcat
    67261 ccgccagtat tccttccgta atttccactc ggtgattcat acccggatga tgcagcacat
    67321 ccattaaaga tcccgcagcg ccagttttcg cgtcacgcgc accaaagacc acgcgaccaa
    67381 tgcgactgtg gatcatcgct ccggcacaca ttacacatgg ttcaagcgtg acatacaacg
    67441 tggcgtcgat cagacgataa ttttgcatca ccagaccacc ctgccgcagg gccatgattt
    67501 ctgcatgtgc ggtgggatca tggcgaccaa tcgggcggtt ccagccttcg ccgattaccc
    67561 gattgttatg cactaatacc gcgccgaccg gcacttcccg ctcatcccag gcacgtttcg
    67621 ccagcgtcag cgcgtgacgc atccagtatt cgtggctaaa ttcgacttca gacaaaaaga
    67681 aaactccggt tataaaagcg cggcgcatta tacacggacg ctatgcttta ctctattcca
    67741 gttgctggag ttcaccgcgc ggggttactc gccaacgatg ctgacagaaa taaagcagcg
    67801 gattgtcctg attactgtcg ctatagccac tgtacagccg cagcggagtg ccgattttgc
    67861 gctccagttg tgcgaccttt tcatgtccca gacaacgcat cgtcaatacc caaccaccat
    67921 agccacgctg aatttggctg gcgataagat taacccgcgg cagccagggc gtatcgaaat
    67981 aaaccgcttc aaccagcggc tgcggagagc cggtaatcaa ccagatatca gcatcggaac
    68041 ttaacaggta ggtggttaat cgctcctgaa ccagcggaaa ggcggtaaca ttgtcgcgaa
    68101 accagcgcac gaaatcggcc tgcaacgtct gtaaacgtgc ttcgctgtga ccaaaagtgc
    68161 acccccacag aagcagactc atcggccagc gtgccgcacg accttttatc aataacgcaa
    68221 tggctataat cggtaacaac ggcaggacaa gtaacgcatt cagcggttgg cgacgcagta
    68281 aatagcgcag aaaactgccg aacatatcct gctgatgcaa tgttccatct aagtcaaaaa
    68341 acaccacacg acgctcgtga gttgccaaac catactcctc tggactgaaa catcctgatt
    68401 aattttcgct actacatagc ctaacagata gatcatcact tttccggcaa caatcgaccg
    68461 catcatggct aactggaatt tttaattcat ggcaattagc ggcaatggaa tataaaattc
    68521 actcgcgtgt ctctcatatt gcctgttgtc gccactattt catcgcgagg aaagatatga
    68581 acggcttact tcgtatccgt cagcgttacc aggggcttgc ccagagtgat aaaaaactgg
    68641 cggattatct gctgctacaa cctgatacgg cgcgccattt aagctctcag caactggcca
    68701 acgaagccgg agtcagtcag tccagcgtcg tgaagttcgc gcaaaaactc ggctataaag
    68761 gttttccggc gcttaagttg gcgttgagtg aagcgctggc aagccagccg gaatcgccct
    68821 ccgtgcccat tcataaccaa atccgcggtg atgatccgtt acggctggtc ggcgaaaaac
    68881 tgattaaaga aaatactgcc gctatgtacg caacgctaaa cgttaatagt gaagagaaac
    68941 tgcatgaatg cgtaacaatg ttgcgctctg cgcggcggat aattctgacc ggtattggcg
    69001 cttcgggtct ggtggcgcaa aactttgcct ggaagctgat gaagattggc ttcaatgctg
    69061 ccgcagtgcg cgatatgcat gcgctactcg caacagtaca ggcgtcgtcc cctgacgatc
    69121 tgttattagc catttcctac accggtgtac gacgcgagtt aaacctggcg gcagatgaga
    69181 tgctgcgagt gggcggaaaa gtgctggcga ttaccggctt tactccgaat gccctgcaac
    69241 agcgtgcttc tcattgcctg tataccattg ccgaagaaca agcgacaaac agcgcttcaa
    69301 tctctgcttg tcacgctcag ggaatgttaa cggatttgct gttcattgcg ctgattcagc
    69361 aggatctgga actggcacca gaacgtattc gtcatagtga agcgctggtg aaaaaactgg
    69421 tctgagtaaa gaatgcgcgt ataatgcccg cccggtttgt gttgttttga gagtttcctt
    69481 atggcgttgt taatcactaa aaaatgcatc aattgtgata tgtgtgaacc cgaatgcccg
    69541 aatgaggcga tttcaatggg agatcatatc tacgagatta acagcgataa gtgtaccgaa
    69601 tgcgtagggc actacgagac accaacctgc cagaaggtgt gcccgatccc caatactatt
    69661 gtgaaagatc cggcgcatgt cgagacagaa gaacagttgt gggataaatt tgtgctgatg
    69721 caccacgcgg ataaaattta atattctact ctggaagtag agtattaatt atattactgg
    69781 gaagccttaa cgccattata ttta
//
LOCUS       DAZFWL020000007        69123 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFWL020000007 DAZFWL020000000
VERSION     DAZFWL020000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 69123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.007"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(60..449)
                     /gene="mutT"
                     /locus_tag="ACHKNP_RS02665"
                     /old_locus_tag="pgaptmp_000533"
     CDS             complement(60..449)
                     /gene="mutT"
                     /locus_tag="ACHKNP_RS02665"
                     /old_locus_tag="pgaptmp_000533"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HGU4702761.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(509..3214)
                     /gene="secA"
                     /locus_tag="ACHKNP_RS02670"
                     /old_locus_tag="pgaptmp_000534"
     CDS             complement(509..3214)
                     /gene="secA"
                     /locus_tag="ACHKNP_RS02670"
                     /old_locus_tag="pgaptmp_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGU4702762.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(3276..3788)
                     /gene="secM"
                     /locus_tag="ACHKNP_RS02675"
                     /old_locus_tag="pgaptmp_000535"
     CDS             complement(3276..3788)
                     /gene="secM"
                     /locus_tag="ACHKNP_RS02675"
                     /old_locus_tag="pgaptmp_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HGU4702763.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(4019..4936)
                     /gene="lpxC"
                     /locus_tag="ACHKNP_RS02680"
                     /old_locus_tag="pgaptmp_000536"
     CDS             complement(4019..4936)
                     /gene="lpxC"
                     /locus_tag="ACHKNP_RS02680"
                     /old_locus_tag="pgaptmp_000536"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HGU4702764.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(5037..6188)
                     /gene="ftsZ"
                     /locus_tag="ACHKNP_RS02685"
                     /old_locus_tag="pgaptmp_000537"
     CDS             complement(5037..6188)
                     /gene="ftsZ"
                     /locus_tag="ACHKNP_RS02685"
                     /old_locus_tag="pgaptmp_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGU4702765.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(6249..7511)
                     /gene="ftsA"
                     /locus_tag="ACHKNP_RS02690"
                     /old_locus_tag="pgaptmp_000538"
     CDS             complement(6249..7511)
                     /gene="ftsA"
                     /locus_tag="ACHKNP_RS02690"
                     /old_locus_tag="pgaptmp_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGU4702766.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(7508..8338)
                     /gene="ftsQ"
                     /locus_tag="ACHKNP_RS02695"
                     /old_locus_tag="pgaptmp_000539"
     CDS             complement(7508..8338)
                     /gene="ftsQ"
                     /locus_tag="ACHKNP_RS02695"
                     /old_locus_tag="pgaptmp_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HGU4702767.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNHAQAEQQ
                     "
     gene            complement(8340..9260)
                     /gene="ddlB"
                     /locus_tag="ACHKNP_RS02700"
                     /old_locus_tag="pgaptmp_000540"
     CDS             complement(8340..9260)
                     /gene="ddlB"
                     /locus_tag="ACHKNP_RS02700"
                     /old_locus_tag="pgaptmp_000540"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU4702768.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(9253..10728)
                     /gene="murC"
                     /locus_tag="ACHKNP_RS02705"
                     /old_locus_tag="pgaptmp_000541"
     CDS             complement(9253..10728)
                     /gene="murC"
                     /locus_tag="ACHKNP_RS02705"
                     /old_locus_tag="pgaptmp_000541"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGU4702769.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISAYNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(10782..11849)
                     /gene="murG"
                     /locus_tag="ACHKNP_RS02710"
                     /old_locus_tag="pgaptmp_000542"
     CDS             complement(10782..11849)
                     /gene="murG"
                     /locus_tag="ACHKNP_RS02710"
                     /old_locus_tag="pgaptmp_000542"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HGU4702770.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(11846..13090)
                     /gene="ftsW"
                     /locus_tag="ACHKNP_RS02715"
                     /old_locus_tag="pgaptmp_000543"
     CDS             complement(11846..13090)
                     /gene="ftsW"
                     /locus_tag="ACHKNP_RS02715"
                     /old_locus_tag="pgaptmp_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HGU4702771.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(13090..14406)
                     /gene="murD"
                     /locus_tag="ACHKNP_RS02720"
                     /old_locus_tag="pgaptmp_000544"
     CDS             complement(13090..14406)
                     /gene="murD"
                     /locus_tag="ACHKNP_RS02720"
                     /old_locus_tag="pgaptmp_000544"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGU4702772.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(14409..15491)
                     /gene="mraY"
                     /locus_tag="ACHKNP_RS02725"
                     /old_locus_tag="pgaptmp_000545"
     CDS             complement(14409..15491)
                     /gene="mraY"
                     /locus_tag="ACHKNP_RS02725"
                     /old_locus_tag="pgaptmp_000545"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGU4702773.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(15485..16843)
                     /gene="murF"
                     /locus_tag="ACHKNP_RS02730"
                     /old_locus_tag="pgaptmp_000546"
     CDS             complement(15485..16843)
                     /gene="murF"
                     /locus_tag="ACHKNP_RS02730"
                     /old_locus_tag="pgaptmp_000546"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGU4702774.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(16840..18327)
                     /gene="murE"
                     /locus_tag="ACHKNP_RS02735"
                     /old_locus_tag="pgaptmp_000547"
     CDS             complement(16840..18327)
                     /gene="murE"
                     /locus_tag="ACHKNP_RS02735"
                     /old_locus_tag="pgaptmp_000547"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HGU4702775.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVTAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(18314..20080)
                     /gene="ftsI"
                     /locus_tag="ACHKNP_RS02740"
                     /old_locus_tag="pgaptmp_000548"
     CDS             complement(18314..20080)
                     /gene="ftsI"
                     /locus_tag="ACHKNP_RS02740"
                     /old_locus_tag="pgaptmp_000548"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HGU4702776.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(20096..20461)
                     /gene="ftsL"
                     /locus_tag="ACHKNP_RS02745"
                     /old_locus_tag="pgaptmp_000549"
     CDS             complement(20096..20461)
                     /gene="ftsL"
                     /locus_tag="ACHKNP_RS02745"
                     /old_locus_tag="pgaptmp_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGU4702777.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(20458..21399)
                     /gene="rsmH"
                     /locus_tag="ACHKNP_RS02750"
                     /old_locus_tag="pgaptmp_000550"
     CDS             complement(20458..21399)
                     /gene="rsmH"
                     /locus_tag="ACHKNP_RS02750"
                     /old_locus_tag="pgaptmp_000550"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGU4702778.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(21401..21859)
                     /gene="mraZ"
                     /locus_tag="ACHKNP_RS02755"
                     /old_locus_tag="pgaptmp_000551"
     CDS             complement(21401..21859)
                     /gene="mraZ"
                     /locus_tag="ACHKNP_RS02755"
                     /old_locus_tag="pgaptmp_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HGU4702779.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(22461..23465)
                     /gene="cra"
                     /locus_tag="ACHKNP_RS02760"
                     /old_locus_tag="pgaptmp_000552"
     CDS             complement(22461..23465)
                     /gene="cra"
                     /locus_tag="ACHKNP_RS02760"
                     /old_locus_tag="pgaptmp_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HGU4702780.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(23547..23633)
                     /locus_tag="ACHKNP_RS02765"
                     /old_locus_tag="pgaptmp_000553"
     CDS             complement(23547..23633)
                     /locus_tag="ACHKNP_RS02765"
                     /old_locus_tag="pgaptmp_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HGU4702781.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(23645..24136)
                     /gene="ilvN"
                     /locus_tag="ACHKNP_RS02770"
                     /old_locus_tag="pgaptmp_000554"
     CDS             complement(23645..24136)
                     /gene="ilvN"
                     /locus_tag="ACHKNP_RS02770"
                     /old_locus_tag="pgaptmp_000554"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU4702782.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(24139..25863)
                     /gene="ilvI"
                     /locus_tag="ACHKNP_RS02775"
                     /old_locus_tag="pgaptmp_000555"
     CDS             complement(24139..25863)
                     /gene="ilvI"
                     /locus_tag="ACHKNP_RS02775"
                     /old_locus_tag="pgaptmp_000555"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HGU4702783.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            25982..26026
                     /locus_tag="ACHKNP_RS02780"
                     /old_locus_tag="pgaptmp_000556"
     CDS             25982..26026
                     /locus_tag="ACHKNP_RS02780"
                     /old_locus_tag="pgaptmp_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702784.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(26181..27125)
                     /gene="leuO"
                     /locus_tag="ACHKNP_RS02785"
                     /old_locus_tag="pgaptmp_000557"
     CDS             complement(26181..27125)
                     /gene="leuO"
                     /locus_tag="ACHKNP_RS02785"
                     /old_locus_tag="pgaptmp_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HGU4702785.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            27786..27872
                     /gene="leuL"
                     /locus_tag="ACHKNP_RS02790"
                     /old_locus_tag="pgaptmp_000558"
     CDS             27786..27872
                     /gene="leuL"
                     /locus_tag="ACHKNP_RS02790"
                     /old_locus_tag="pgaptmp_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HGU4702786.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            27965..29536
                     /gene="leuA"
                     /locus_tag="ACHKNP_RS02795"
                     /old_locus_tag="pgaptmp_000559"
     CDS             27965..29536
                     /gene="leuA"
                     /locus_tag="ACHKNP_RS02795"
                     /old_locus_tag="pgaptmp_000559"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HGU4702787.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            29536..30627
                     /gene="leuB"
                     /locus_tag="ACHKNP_RS02800"
                     /old_locus_tag="pgaptmp_000560"
     CDS             29536..30627
                     /gene="leuB"
                     /locus_tag="ACHKNP_RS02800"
                     /old_locus_tag="pgaptmp_000560"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGU4702788.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            30630..32030
                     /gene="leuC"
                     /locus_tag="ACHKNP_RS02805"
                     /old_locus_tag="pgaptmp_000561"
     CDS             30630..32030
                     /gene="leuC"
                     /locus_tag="ACHKNP_RS02805"
                     /old_locus_tag="pgaptmp_000561"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HGU4702789.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            32041..32646
                     /gene="leuD"
                     /locus_tag="ACHKNP_RS02810"
                     /old_locus_tag="pgaptmp_000562"
     CDS             32041..32646
                     /gene="leuD"
                     /locus_tag="ACHKNP_RS02810"
                     /old_locus_tag="pgaptmp_000562"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGU4702790.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(32695..33873)
                     /gene="setA"
                     /locus_tag="ACHKNP_RS02815"
                     /old_locus_tag="pgaptmp_000563"
     CDS             complement(32695..33873)
                     /gene="setA"
                     /locus_tag="ACHKNP_RS02815"
                     /old_locus_tag="pgaptmp_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HGU4702791.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(33975..34106)
                     /gene="sgrT"
                     /locus_tag="ACHKNP_RS02820"
                     /old_locus_tag="pgaptmp_000564"
     CDS             complement(33975..34106)
                     /gene="sgrT"
                     /locus_tag="ACHKNP_RS02820"
                     /old_locus_tag="pgaptmp_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HGU4702792.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            34195..35850
                     /gene="sgrR"
                     /locus_tag="ACHKNP_RS02825"
                     /old_locus_tag="pgaptmp_000565"
     CDS             34195..35850
                     /gene="sgrR"
                     /locus_tag="ACHKNP_RS02825"
                     /old_locus_tag="pgaptmp_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HGU4702793.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      35884..35983
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            36014..36997
                     /gene="thiB"
                     /locus_tag="ACHKNP_RS02830"
                     /old_locus_tag="pgaptmp_000566"
     CDS             36014..36997
                     /gene="thiB"
                     /locus_tag="ACHKNP_RS02830"
                     /old_locus_tag="pgaptmp_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HGU4702794.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            36973..38583
                     /gene="thiP"
                     /locus_tag="ACHKNP_RS02835"
                     /old_locus_tag="pgaptmp_000567"
     CDS             36973..38583
                     /gene="thiP"
                     /locus_tag="ACHKNP_RS02835"
                     /old_locus_tag="pgaptmp_000567"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HGU4702795.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            38567..39265
                     /gene="thiQ"
                     /locus_tag="ACHKNP_RS02840"
                     /old_locus_tag="pgaptmp_000568"
     CDS             38567..39265
                     /gene="thiQ"
                     /locus_tag="ACHKNP_RS02840"
                     /old_locus_tag="pgaptmp_000568"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HGU4702796.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(39379..40143)
                     /gene="yabI"
                     /locus_tag="ACHKNP_RS02845"
                     /old_locus_tag="pgaptmp_000569"
     CDS             complement(39379..40143)
                     /gene="yabI"
                     /locus_tag="ACHKNP_RS02845"
                     /old_locus_tag="pgaptmp_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4702797.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(40229..41107)
                     /gene="araC"
                     /locus_tag="ACHKNP_RS02850"
                     /old_locus_tag="pgaptmp_000570"
     CDS             complement(40229..41107)
                     /gene="araC"
                     /locus_tag="ACHKNP_RS02850"
                     /old_locus_tag="pgaptmp_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HGU4702798.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRYPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            41446..43146
                     /gene="araB"
                     /locus_tag="ACHKNP_RS02855"
                     /old_locus_tag="pgaptmp_000571"
     CDS             41446..43146
                     /gene="araB"
                     /locus_tag="ACHKNP_RS02855"
                     /old_locus_tag="pgaptmp_000571"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HGU4702799.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            43157..44659
                     /gene="araA"
                     /locus_tag="ACHKNP_RS02860"
                     /old_locus_tag="pgaptmp_000572"
     CDS             43157..44659
                     /gene="araA"
                     /locus_tag="ACHKNP_RS02860"
                     /old_locus_tag="pgaptmp_000572"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HGU4702800.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            44828..45523
                     /gene="araD"
                     /locus_tag="ACHKNP_RS02865"
                     /old_locus_tag="pgaptmp_000573"
     CDS             44828..45523
                     /gene="araD"
                     /locus_tag="ACHKNP_RS02865"
                     /old_locus_tag="pgaptmp_000573"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGU4702801.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            45598..47949
                     /gene="polB"
                     /locus_tag="ACHKNP_RS02870"
                     /old_locus_tag="pgaptmp_000574"
                     /pseudo
     CDS             45598..47949
                     /gene="polB"
                     /locus_tag="ACHKNP_RS02870"
                     /old_locus_tag="pgaptmp_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
     gene            48114..51020
                     /gene="rapA"
                     /locus_tag="ACHKNP_RS02875"
                     /old_locus_tag="pgaptmp_000575"
     CDS             48114..51020
                     /gene="rapA"
                     /locus_tag="ACHKNP_RS02875"
                     /old_locus_tag="pgaptmp_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HGU4702802.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            51032..51691
                     /gene="rluA"
                     /locus_tag="ACHKNP_RS02880"
                     /old_locus_tag="pgaptmp_000576"
     CDS             51032..51691
                     /gene="rluA"
                     /locus_tag="ACHKNP_RS02880"
                     /old_locus_tag="pgaptmp_000576"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HGU4702803.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYVADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(51808..52623)
                     /gene="djlA"
                     /locus_tag="ACHKNP_RS02885"
                     /old_locus_tag="pgaptmp_000577"
     CDS             complement(51808..52623)
                     /gene="djlA"
                     /locus_tag="ACHKNP_RS02885"
                     /old_locus_tag="pgaptmp_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HGU4702804.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            52878..55232
                     /gene="lptD"
                     /locus_tag="ACHKNP_RS02890"
                     /old_locus_tag="pgaptmp_000578"
     CDS             52878..55232
                     /gene="lptD"
                     /locus_tag="ACHKNP_RS02890"
                     /old_locus_tag="pgaptmp_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HGU4702805.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            55285..56571
                     /gene="surA"
                     /locus_tag="ACHKNP_RS02895"
                     /old_locus_tag="pgaptmp_000579"
     CDS             55285..56571
                     /gene="surA"
                     /locus_tag="ACHKNP_RS02895"
                     /old_locus_tag="pgaptmp_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HGU4702806.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            56571..57560
                     /gene="pdxA"
                     /locus_tag="ACHKNP_RS02900"
                     /old_locus_tag="pgaptmp_000580"
     CDS             56571..57560
                     /gene="pdxA"
                     /locus_tag="ACHKNP_RS02900"
                     /old_locus_tag="pgaptmp_000580"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HGU4702807.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            57557..58378
                     /gene="rsmA"
                     /locus_tag="ACHKNP_RS02905"
                     /old_locus_tag="pgaptmp_000581"
     CDS             57557..58378
                     /gene="rsmA"
                     /locus_tag="ACHKNP_RS02905"
                     /old_locus_tag="pgaptmp_000581"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGU4702808.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            58381..58758
                     /gene="apaG"
                     /locus_tag="ACHKNP_RS02910"
                     /old_locus_tag="pgaptmp_000582"
     CDS             58381..58758
                     /gene="apaG"
                     /locus_tag="ACHKNP_RS02910"
                     /old_locus_tag="pgaptmp_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HGU4702809.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            58765..59613
                     /gene="apaH"
                     /locus_tag="ACHKNP_RS02915"
                     /old_locus_tag="pgaptmp_000583"
     CDS             58765..59613
                     /gene="apaH"
                     /locus_tag="ACHKNP_RS02915"
                     /old_locus_tag="pgaptmp_000583"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HGU4702810.1"
                     /translation="MATYLIGDVHGCYDELFALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            59638..60237
                     /locus_tag="ACHKNP_RS02920"
                     /old_locus_tag="pgaptmp_000584"
     CDS             59638..60237
                     /locus_tag="ACHKNP_RS02920"
                     /old_locus_tag="pgaptmp_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HGU4702811.1"
                     /translation="MADYFEIRADYNQHTITIYQAYNDAIADVAVRDGRFGAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            60273..60740
                     /locus_tag="ACHKNP_RS02925"
                     /old_locus_tag="pgaptmp_000585"
     CDS             60273..60740
                     /locus_tag="ACHKNP_RS02925"
                     /old_locus_tag="pgaptmp_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702812.1"
                     /translation="MRQINTGLNTREIILEMIDEADAPPFAEQRTWHRRRIAFLCDCI
                     SVAVDYGFFCSVSQQSIMNITKMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYAQK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADVIQLRCDV"
     gene            complement(60839..61018)
                     /locus_tag="ACHKNP_RS02930"
                     /old_locus_tag="pgaptmp_000586"
     CDS             complement(60839..61018)
                     /locus_tag="ACHKNP_RS02930"
                     /old_locus_tag="pgaptmp_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HGU4702813.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(61039..61518)
                     /gene="folA"
                     /locus_tag="ACHKNP_RS02935"
                     /old_locus_tag="pgaptmp_000587"
     CDS             complement(61039..61518)
                     /gene="folA"
                     /locus_tag="ACHKNP_RS02935"
                     /old_locus_tag="pgaptmp_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HGU4702814.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(61710..63572)
                     /gene="kefC"
                     /locus_tag="ACHKNP_RS02940"
                     /old_locus_tag="pgaptmp_000588"
     CDS             complement(61710..63572)
                     /gene="kefC"
                     /locus_tag="ACHKNP_RS02940"
                     /old_locus_tag="pgaptmp_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HGU4702815.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(63565..64095)
                     /gene="kefF"
                     /locus_tag="ACHKNP_RS02945"
                     /old_locus_tag="pgaptmp_000589"
     CDS             complement(63565..64095)
                     /gene="kefF"
                     /locus_tag="ACHKNP_RS02945"
                     /old_locus_tag="pgaptmp_000589"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HGU4702816.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(64203..65534)
                     /gene="yaaU"
                     /locus_tag="ACHKNP_RS02950"
                     /old_locus_tag="pgaptmp_000590"
     CDS             complement(64203..65534)
                     /gene="yaaU"
                     /locus_tag="ACHKNP_RS02950"
                     /old_locus_tag="pgaptmp_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4702817.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(65593..65880)
                     /gene="fixX"
                     /locus_tag="ACHKNP_RS02955"
                     /old_locus_tag="pgaptmp_000591"
     CDS             complement(65593..65880)
                     /gene="fixX"
                     /locus_tag="ACHKNP_RS02955"
                     /old_locus_tag="pgaptmp_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HGU4702818.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(65877..67163)
                     /gene="fixC"
                     /locus_tag="ACHKNP_RS02960"
                     /old_locus_tag="pgaptmp_000592"
     CDS             complement(65877..67163)
                     /gene="fixC"
                     /locus_tag="ACHKNP_RS02960"
                     /old_locus_tag="pgaptmp_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4702819.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(67214..68155)
                     /gene="fixB"
                     /locus_tag="ACHKNP_RS02965"
                     /old_locus_tag="pgaptmp_000593"
     CDS             complement(67214..68155)
                     /gene="fixB"
                     /locus_tag="ACHKNP_RS02965"
                     /old_locus_tag="pgaptmp_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU4702820.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(68170..68940)
                     /gene="fixA"
                     /locus_tag="ACHKNP_RS02970"
                     /old_locus_tag="pgaptmp_000594"
     CDS             complement(68170..68940)
                     /gene="fixA"
                     /locus_tag="ACHKNP_RS02970"
                     /old_locus_tag="pgaptmp_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HGU4702821.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        16695 a        19223 c        17433 g        15772 t
ORIGIN      
        1 cagcgtcgca tcaggcatct tgtgcaaatg tcggatgcgg cgtgaacgcc ttatccgacc
       61 tacagacgct taagcttcgc aattaccggt tcattggctg gcggaaaatc atcggcatta
      121 agaccgacta gcgacatcca ctcaccgggt tgcccttctt taccccacgg ctccccttcc
      181 cagctttcga ccagccagaa ccacagagtt atatgcctgt ccgggaattc atattccagt
      241 ttttcaaata gcgaaaaatg ttgtggggta atcccgactt cttcctgaag ttcacgcacc
      301 accgcctgtt ccggcgtttc gcccatttca attttaccgc cgggaaactc cagtttattc
      361 gccatgtgcg catctgctgc gcgacgcgtt ataaagattt cattgttctc gttgcgaata
      421 atacctaccg caatttgcag ctttttcatt gtcttaaacc tataaaaaag gcgcagaatc
      481 ctgcgccttt tacttcaact gttagctttt attgcaggcg accatggcac tgcttgtatt
      541 ttttaccaga accgcacggg caaggatcgt tacgtcctac tttgcgctca ccggtttgcg
      601 ccgccagtgc agctgcggct gcagagtcgt catcctgatg gctaagctgc tgcatttgcg
      661 ctaaacgctc ggcttccata cgacgctgtt gttccagctc ctcaacctct tcaggcatac
      721 gtacctggac tttgctcagc gtgctgataa cttcatattt taacgactcc agcatcgctg
      781 caaacatgga gaacgattca cgtttgtatt cctgcttcgg atctttctgt gcatagccac
      841 gcaggtggat accctgacgc agatagtcca tcgctgccag gtgctctttc cacagggagt
      901 caagcgtttg cagcatgacg cctttctcga agtgacgcat catctcagca ccaaccactt
      961 cttctttacg ctgatacact tcgatggact gcgccagaat gcgctcacgc agcgtctctt
     1021 catgcagttc tggttcttta tccagccact cggcaattgg taaatcgagg tcgaaatcgt
     1081 tcttcagacg ttcctgcagc cccggaatat cccacatttc ttccagcgac tgtggcggaa
     1141 tgtaggcatc aatggtggct ttgaacacat cttcacgaat gctgttgatg gtttcgctca
     1201 catcgctgac atccagcagt tcgttacgct gggagtaaat ggcgcgacgc tgatcgttag
     1261 ccacgtcatc atattccagc agttgcttac gaatgtcgaa gttacgactt tcaactttac
     1321 gctgggcgtt ggcaatcgct ttagtcaccc acgggtgttc aatggcttcg cctggcttca
     1381 tacccagttt acgcatcatg ccggatactc ggtcggaagc aaaaatacgc atcagcgcat
     1441 cttccatcga caggtagaaa cgggaagaac cagcatcccc ctgacgacca gaacgaccgc
     1501 gcaactggtt atcgatacga cgggattcgt gacgctcggt accgatgata tgcaggccac
     1561 ctgcttccag taccgcatcg tgacgtacct gccagtcggc tttaattttt tcaatttgct
     1621 ctacggtcgg attttccagc gcggcaactt ctgcctgcca gctaccaccg agcacaatat
     1681 ctgtaccacg acccgccata ttggtcgcga tagtcaccgc agccggataa cctgcctgag
     1741 caacaatcgc cgcttcgttg gcgtggaatt tggcgttcag gacgttgtgc ttaataccgg
     1801 ctttggtcag ttcgtttgac accagctccg atttttcgat ggagatagta cccaccagca
     1861 ccggctggcc tttcgcagta cgttctttga tatcttcaat gatcgcctga attttttccg
     1921 cttcagtcat gtagaccagg tccggcagat ctttacgaat cattggacgg ttggtcggaa
     1981 caacgacggt atccagcttg tagatggagc taaattcgaa agcttcggta tcagcagtac
     2041 cggtcatccc cgccagtttt tcatacagac ggaagtagtt ctggaaggtg atcgaagcca
     2101 gcgtttggtt ttcgttctgg atctgcacac cttctttcgc ttccacagcc tggtgcagac
     2161 catcggacca gcgacggccc tgcatggtac gaccggtgtg ttcgtcaacg atgataactt
     2221 caccatcttt aacgatgtag tcgacgtcgc gggtaaacag cgcatgagcg cgcagcgccg
     2281 ccgttacgtg gtgcatcagc atgatgttgg ccggagagta cagagactcc ccttcatcca
     2341 tgatgccctc tttcaccagc agttcttcaa tcagcaccag accacgttcg gtcaggttca
     2401 cctggcgaga tttttcgtcc accgagaagt ggccttcgcc ctggaaggtt tcggagtctt
     2461 ctttttcctg acggatcagg tgcggaataa ttttattcac gcgtttatac atttccgagc
     2521 tgtcttctgc cgggccggaa atgatcagcg gtgtacgcgc ttcatcgatc aggatggagt
     2581 ccacttcgtc caccagcgca tagtgcagtt tacgctgtac acgttcttca gggctgaacg
     2641 ccatattgtc gcgcaggtag tcaaagccgt attcgttgtt cgtaccgtaa gtgatgtcag
     2701 ctgcgtaagc ttcgcgcttt gccggtgctg gcatgcccgg caggttgata ccgacagtca
     2761 ggccaaggaa ttcaaacagc ggacggttgt tttcggcgtc acgttgcgcc aggtagtcgt
     2821 tgacggtaac tacgtgcacg cctttaccgg ttagtgcgtt caggtaagca ggcagcgttg
     2881 cggtcagggt tttaccttca ccggtacgca tttcggcgat gcagcgttcg ttaagaacca
     2941 taccgccgag taactgaacg tcgaagtgac gcataccaaa gacgcgctta cttgcctcac
     3001 gtaccacggc gaaagcttcc gggatcagat tttccagcac ttcgcctttt tccagacgtg
     3061 cacgaaactc tgcggttttc cctttcagtt cttcgtcgga gagtttttcc atctccggtt
     3121 ccatggcatt gatgatgttg accactttgc gcatccggcg cagggtgcga tcgttacgac
     3181 taccgaaaac tttagttaac aatttgatta gcataataaa atctcaaacg ccccgcgttg
     3241 cggagttaat aaaatgaagt aaaggtttat tgttgttagg tgaggcgttg agggccagca
     3301 cggatgcctt gcgcctggct tatccagacg ggcgtgctga attttgcttg tggggtaaaa
     3361 tgcgcataat caatgcgata acccttttca gacggcgtgc cttcctgggt cagcagcgcg
     3421 ctgagcgtat ccagtaatgc aagatgttgc gcctgaagag gcaaagattc ttcagcaacg
     3481 ggcagtgttt gcggtgccat tgcgaaagaa agatgacgga ttaccgtgcg aatggcatgt
     3541 tgatgccagt aatcaacgga atagttcgaa ttcgggcggc gtgtgttcgc ttccagcaag
     3601 gccaattgac caaagttaac tttggctgaa ggctcgtggt tgcgggttgt cgcttttgcg
     3661 ggcgcgtttg gttcggcggc gttgctgagc gcaggcaaac ctaaactcgc cgcaaccatc
     3721 cctaataaga gatgcggcca gaagtagcgt ttaccaaact gtcgccagcg cgtcagtatt
     3781 ccactcacgt tattgccatc ccgatcgtat tcaaacgtat ttagcgcaca aatcttatca
     3841 ttaaagcctg cagtccacag cacgaatgaa gataagttgt gcggaaaagt gcttaagaaa
     3901 acagcgttcg caccgttttt tatagctctt ataaaattta aaaaaaaacg actggttcac
     3961 ctggccggag agagtgccag atttgccagt cgaattttat acgacagtat aaatgtcgtt
     4021 atgccagtac agctgaaggc gctttgaagg ccaacggcag ttctgcgtcg tcctggaagg
     4081 tcacatattc ccaggcttcc tgtttcgcca ggacagcctg cagcagtttg ttattcagtg
     4141 catgaccgga tttataagcg gtaaatgcac caataatatt gtgaccacac atgaacaagt
     4201 caccgatcgc atcgagcatt ttgtgacgca caaattcgtc ttcaaaacgc aggccgtctt
     4261 cgttcagtac gcgataatcg tcaacaacga tggcacaatc gaagctgccg cccaggcaca
     4321 aaccacggga ctgcagatat tcgatatcac gcatgaaacc gaacgtacgc gcacggctga
     4381 tctggcgcat aaacgcatca gcggagaagt tcatcgcata gcgctggttg ctggaatcaa
     4441 tagccggatg gttaaaatcg atggtgaaat ccagcgaaaa accattgtac ggcttaaatt
     4501 cagcccactt atcgccatct tcgacacgaa cagtctcttt gatgcgaaca aattttttgg
     4561 cgcagttcaa ctcgtcgata ccggcgtcaa gcagcaggta tacaaacgga gcggcgctgc
     4621 cgtccatgat cgggatttcc ggcgcgttaa cttcgataac aatgttatcg atgcccaagc
     4681 ccgcgagagc agcattgagg tgctctacgg ttgaaatccg tacatcatgc tcgttgacca
     4741 gacacgtaca gagcatggta tcacgcacag atttggcatc ggccgggaaa tctaccggtg
     4801 gattcaagtc ggtgcgacga tagatgaccc cggtgttggc cggcgcaggg cgtaacgtca
     4861 gggtgacttt cttgccggta tgtaaaccga cacccgtcgc ctgaacgata cgtttaagtg
     4921 tcctttgttt gatcatcgta ttatctcgcc aaattaccta tccaaccgaa gtgtactata
     4981 cattcggcgg gccagtttag cacaaagagc ctcgaaaccc aaattccagt caattcttaa
     5041 tcagcttgct tacgcaggaa tgctgggata tccagataat ccggctcttt cgcagtttgc
     5101 ggcgcattgt cattcacgac tttagcaacc ggcttctgct cctgggtcag cggagccatc
     5161 ccatgctgct ggtagcgatc catcactggc tgctgaacct gcttattggt caccagagtg
     5221 atttcaggac gtttgtccat gccgatacct gtcgcaacaa cggttacgcg cagctcgtca
     5281 ttcatatccg ggtcaagaga agtaccgata accacagtcg cgttgtcgga agcaaatgca
     5341 cggatggtgt tacctaccgt ttcgaactca tccagacgca ggtcgaagcc cgccgtgatg
     5401 ttaaccagca cgccgcgcgc gccagacagg tcgatatctt ccagcagcgg agaagagata
     5461 gccatttcag cagcttcttc cgcacggtct tcaccgctcg ccacgccaga acccatcatt
     5521 gcgtagccca tctcagacat tacggtgcgt acgtctgcaa agtccacgtt catcaaaccc
     5581 ggacgagtaa tcagttcagc gataccttgc acagcgcctt tcagtacatc gttcgctgcg
     5641 ccaaacgcat ccagcaggga gataccgcgg cccagaactt tcagcagttt gtcgttcggg
     5701 atagtgatca gagagtccac atgcttggac agttcagtga tcccctgctc cgcgaatgcc
     5761 atacgcttct tgccttcaaa gttgaaaggc ttagtgacga cagcaacggt caggataccc
     5821 aaatcttttg ccacttcagc gacgactggt gctgcacctg taccggtacc accacccata
     5881 cccgcagcaa taaagaccat gtctgcacct tccagcgccg cacgcaatgc atcgcgatcc
     5941 tcatcagccg cattgcggcc aacttctgga ttagcgccag cgcccagtcc tttggtgata
     6001 ccgctaccga tttgaatcgt ctgtccaacc gctgttttac gcagcgcttg tgcatcggta
     6061 tttaccgcga agaattcaac accttcaatg cgctcgcgca ccatgtgttc aacagcatta
     6121 ccgccgccgc cgccgacgcc gatgacttta atcaccgcgt cattggtaag ttccattggt
     6181 tcaaacatag tttctctccg atttgtgcct gtcgcctgag gccgtaatca tcgtcggcct
     6241 cataaaaatt aaaactcttt tcgcagccaa ctattgagtc gcttgatcca cgagccaact
     6301 gatgctgtaa cacgtttttc tacttcagct tcaccgttaa gatgtgactc tttcccatag
     6361 tgaagcaatc ccaccgccgt cgaataatac ggctcctgag cataatccgt taaaccggta
     6421 atgttcagcg gcgcgccgat acgcacttgc gtatgaaaca cgcgctgagc acaggctgca
     6481 agaccttcga tctgcgctgc gccaccggtt aatacaatgc ctgccgccag gtgatgttta
     6541 accccttgtt ggcgaagctt ttcctgcaac tgcaatatct cttcgttgac caggttgagc
     6601 agctcggtat agcgcggctc gatcacctct gccagggtct gacgttgcag actccgcggc
     6661 ggacgaccac ctacgctcgg cacttccacg ctctcatctt ttccaacgat ggaacccagc
     6721 gcacaaccgt ggcgaacttt aatcgcttcg gcgtcgcttg gcggcgtgcc aaaggcgtaa
     6781 gcgatatcac tggtcacgac attgccagca taaggaatta ccttagtgtg gcgcaatgcc
     6841 ccaccggtat aaacggcgat atccattgta ccaccaccga tatcgacgac gcagacaccc
     6901 agttcacgtt catcttccgt caataccgaa taacttgatg ccagtccggc aaatatcagt
     6961 tggtcaactt tcagcccaca acgttcaacc gctttgacga tgtttttcgc catatcgttg
     7021 tgacatgtga tcaggtgcac ttttgcctgc atccgcacgc ccgaaagtcc taccggattc
     7081 ttgatccctt cctgatagtc aatcgcatac tcttgcggga tcacatgcag cacacgatgc
     7141 tcatcgcgca cacgcaccga tttcgcggta tggacgacgt tttccacatc ttcttgcgtc
     7201 acttcttctt cagaaatagg caccatacca atttcattct ggcagctgat gtgcttacca
     7261 gaaagcgcca gatataccga agagatctga caatctgcca tcaattctgc ctggtcaatg
     7321 gcgcgttgta cgcacttgac cacggattcg aggtcgttca ccccgccttt atccatacca
     7381 cgtgacgggc agctgcccac gccaatgata ttgaccatac cgtcgggcag aacttcccct
     7441 actaaagcgg caaccttcgc ggtaccaatc tccagtccta ctaccagttt tctgtccgtc
     7501 gccttgatca ttgttgttct gcctgtgcat gattttgttg ctgagtagat tcctctggcg
     7561 gcaagggcgc ccagcctact gccgctccag agtcataacg caaatcaacg tagctaatcc
     7621 gtttgccatc ggtttgcgcc tgctgctgta aaaccggata aagttctaca aagcgagcca
     7681 aacgtttcat cgtatcgccc cggccaagat tgagcttaat atcgttattc agcgtcaact
     7741 gccaggaacg ccgcgcggtc atcgccgctt ccttcagagt aaatctgtcc tttgccagca
     7801 tctgccccat ttcgcgatag ccctgcaaca cttcattggc gctgccttcc gggccataca
     7861 gcattggaag cacctgcttg ctggtgcgat ctggcggcac gctgaaggtg tttccttccg
     7921 cgtctaccat atgttgatca ttccaccgcg caatcggcac atattcaacc agatgaatct
     7981 tcaattcatc aggccactgc tttctgacgc tcacctgctt aatccacggc aggcgttgtt
     8041 ctatttgcgt ctggatgatg ttgacatctt gggtcataaa ggtacccggc tcacccaatg
     8101 ccaggatcga ctgccggata tcgtcattac gcgtgtaatg gcgttcaccg gtcaacacca
     8161 gctttgagag cggcaggcgt tgcgcatctt ccatccagcc caacacgacc cagccgctca
     8221 ccaacactgt cgttaaaacg gtcagcagga aaaggatccc cgccagacgc gttccattat
     8281 tgcggcgaga agaaacctct tcttcgctat ttcgcgtgtt cagagcagcc tgcgacatat
     8341 tagtccgcca gttccagaat tcgtactacc aactgcgaga agctcatacc tgcctgacgt
     8401 gccgccatcg gcaccaggct gtggctggtc atacccggtg aggtattggc ttccagcaga
     8461 taaaactgtc catcgctgtc cagcataacg tcaatacgtc cccatccttt gcaacctaac
     8521 gtcgtccatg ctttcagcac taatgcctgc aaattggcct cttgtgacgc ttccagacct
     8581 gcggggcaga aatactgtgt ctcatcagag agatacttcg cctcataatc atagaaggtt
     8641 ccggacggtt gaatacgtat tgacggtaaa atttcttcac cgagtatcgc aaccgtgaac
     8701 tccggcccac ttagccattt ttcaatcaat acttcttcat cgtgctgaaa tgccaatctt
     8761 aatgcatctt gtagagcatt ttctgctact acttttgaca ttcccacact ggaaccttcg
     8821 cggctcggct taacgataac cggcaaaccc agagcagaaa tttctgctaa ctgcttatcg
     8881 ctcaggcctt tttcaaactc tgcgcgggtt aacgctaccc acggcgcgac cggtaaaccg
     8941 gcaccttgcc acagcagttt gctgcgtagt ttatccattg aaagcgcaga tgccatcact
     9001 ccgctgccgg tataaggcaa gcccatcagc tcgagcatgc cctgcagcgt accatcttca
     9061 ccgccgcgac cgtgtagcgc gataaacact ttctgaaaac ccatcgactt cagttgcgtc
     9121 acgtcgactt ctttcgggtc gacaggatac gcatcaatac cgccttcacg cagcccggct
     9181 aacaccgctg cgccagaatt cagagaaact tcccgctcag cggaagtccc gcccaacagg
     9241 accgcgattt tatcagtcat gttgttcttc ctccggagtt tgcggcttca gtttgatttc
     9301 agctaaagaa cgggcaattt ttccgatatt accagccccc tgaacgagaa tcaggtcgtt
     9361 accggttaat accggtgcca gcatctcggc tacctgcgca gggtccggca ccagaatggg
     9421 atcaattttc ccacgtccac gaattgtgcg acacagcgaa cggctgtccg ctcccggaat
     9481 tggcgcttcg ccagccggat acacttccag catcaacagg gtatcaacct gcgtcagcac
     9541 attggcgaaa tcatcataca ggtcgcgcgt acgggtaaaa cggtgcggct gaaacagcat
     9601 taccaggttt ttatccggcc agcctgcgcg cgccgcttta atggtggcgt caacttccgt
     9661 cgggtggtgg ccgtagtcat cgaccagcat tgccgtaccg cttttaccat tcactggctc
     9721 cagcgggaat tcaccgagga aatcaaaacg gcgcccagtc ccctggaagc tctccagcgc
     9781 acgtaaaata gcctcgtcat caattccctc ttccgtagca accgcaaccg cagctgcggc
     9841 gttcagcgcg ttatgacgac ctggcgcatt cagggtgacg cgcatcggct ctttgtcctg
     9901 gcgcagcagc gtaaagtgcc cctgcgggcc aatctgctga taatcttcta cacgcacgtc
     9961 ggcatcttcg ctgaagccgt aagtcgtggt ctgacgtccc actcgcggta acaattcgcg
    10021 gatcaccgga tcatcaacac acatcaccgc acgaccgtaa aacggcaggt tgtgcagaaa
    10081 attaataaaa gtctgtttta aattctcaaa gtcgccctgg taggtatcca tgtggtcggc
    10141 ttcgatattg gtgacaatcg ccaccatcgg ttgcagatgc aggaacgatg catcactctc
    10201 atctgcttcg gcaatcaggt accgaccatg ccccaaacgc gcatgaaccc ccgccgcttt
    10261 taccagcccg ccgttaacga aggttgggtc gagccccgct tctgcgtaga tgctggaaac
    10321 catcgcggtg gttgtcgttt tgccgtgcgt tcctgcaatg gcgatgccat gacgaaaacg
    10381 cattaactca gccagcattt cggcacgacg gatcaccgga atacgcgctt catgagctgc
    10441 gacaatttcc gggttatagg cagaaatggc gctggaaaca acgaccacgc tggcatcacg
    10501 tacgttttcc gggcgatggt tgaaataaat cgtcgctccc agattcatta actgctgcgt
    10561 gaccggattt ggcgctaaat cggaaccact gatctgataa ccttcattgg ccagaacttc
    10621 ggcaataccg cccataccgg caccaccgat gccgacaaaa tgtatgtgcc gaacgcgacg
    10681 catttcgggc acgatggaac gcagttttgc caattgttgt gtattcattc tttacgccat
    10741 taacttctta aattcatacg atgcaaaagg catcgctaca attacgcccg ggcagcccgg
    10801 ctcacttcat ttgccactcg ctcggtggca tccggaatgg atgcagcgcg ggcgcgttct
    10861 gccatggtta ataaggtttc tcgcgaccac ccggccaggg tgttggcgac agcatccacg
    10921 ctaagctgtg gctgctcgat aattttggct gcgcccgctt tttccagcgg tagcgcattc
    10981 cagtattgct ggcggtcttt atgttgaaac ggcacaaaca acgccggtag tcctgccgcg
    11041 gcgatttcac tcaccgttaa agcaccggag cggcaaacga cgacatccgc ccacgcatac
    11101 gccgccgcca tatcatcaat aaattccgtc actttatgct gcggttgccc cgcttcggca
    11161 tacgcctgtt caacggattg ttgcgaacct ttaccgctct gatgccagat agtgactgaa
    11221 tcacccagtt tcgcagcaac ctgcggcatt gtctggttaa gaatgcgtgc gccctgggaa
    11281 ccacccacta ccagcacacg aaccggacct tcacgtccag ccaaacgttg ctgcggcaac
    11341 ggcagcgcca gtacatcggt acgcaccggg ttacccacca cttccgcatt agggaaagcg
    11401 cctggaaacg cctgcatcac tttggtggca atcttcgcca gccatttatt ggttaagccc
    11461 gcaataccgt tttgttcatg aagtacaacc ggaatgccta acgaccacgc ggccagacca
    11521 cctggacctg atacatagcc gcccataccg agtaccacgt caggtttgta cgctttcata
    11581 atcgcccgcg cctgacgcca ggcgttgaag atacgcagcg gcgcagctat cagtgctttt
    11641 ataccttttc cacgcagacc agagatacga atgaaatcaa tttcgatgcc atgttttggc
    11701 actaagtccg cttccatacg gtcggcagtc cccagccagc gaacttgcca accctgagcc
    11761 attagatggt gcgcaaccgc cagtcccggg aatacatgtc caccggttcc gcctgccatc
    11821 accattaatc gctttccttg accactcatc gtgaacctcg tacaaacgcc tgcgctttct
    11881 ccagacgcgt ttcataatca atacgcaaca gcatcatgat ggctgtcgac ataatcagta
    11941 agctcgaacc accgtaactg atcagcggca atgtcagacc tttggtcggt aacatccccg
    12001 ccgccgcgcc tacgttaacc agcgcctgga agctaaacca gatgccaata gaacaggcga
    12061 gaaaaccgga aaaacggtgg tcaatttcta acgctttgcg gccaatcgac atcgcgcgaa
    12121 aagcgacgaa gaataccatt aaaagtgcca gcaccacacc gacatacccc agttcttcgc
    12181 cgataatggc gaaaataaag tcagtgtgcg cttccggcag atactccagt ttttgtaccg
    12241 agttacctaa accttgtccc caaagttcgc cgcgaccaaa cgccatcagc gattgcgtta
    12301 actgatagcc gctgccaaag ggatcttccc acgggttcca gaatgcggta acacggcgga
    12361 tacggtacgg ttcggcgagt atcagcaaca caaccgctga aatgcccata ccgataatgg
    12421 caatgaactg ccacaatttc gctcccgcca ggaacaacat cgccagcgta gtcacaaaca
    12481 acaccaccac cgtaccaagg tctggctgtg ccagcagtaa cactgccaac accagaatca
    12541 cgcccatcgg tttcaggaag ccgcgcaggt tattacgtac ttcgtcgcct ttacgcacca
    12601 gatagttggc gatataacaa aacagcgaca gttttgtcag ctccgcaggc tggatacgca
    12661 gcaaaccgag atcgatccaa cgcgatgccc ctttaaccga gctacccact accagcacga
    12721 tcatcagcag gatgatagat ccgagcagca tcgtggcact gtagcgttgc cagaactcca
    12781 tcggcagacg cagcgtaatg atcgccagaa tgaatgccag gatcagatag acgccatcac
    12841 gcttcgcgaa gaagaacgga tcgttggtta agcgttgccc tatgggcatt gacgccgagg
    12901 tcaccatgat aaagccaatc gccgcaaggc cgaaggtcag ccacagtaag gtgcgatcgt
    12961 acatgatcag gctgtcggta tctttttccc gcgagcccat cacccagcct tttagcgccg
    13021 tggagatcca gaccaggata ctgaatcctg gcaggcgcgg cattttcagg cgagggagag
    13081 ataaacgcat caacctaact ccttcgccag acgggcaaac tcattgcctc gttgttcaaa
    13141 gttcttgaac tgatcaaggc tggcacaggc cggggagagc agaaccatat cgcccggctg
    13201 aacacgcgga gccagcaagc gcatcgcctg ttccatagtt tcggtttgtt ctgccacttc
    13261 cgggcgtagc gccgccagct gcgcaccgtc acgaccaaaa cagtacaaac gcacgttgtc
    13321 gccattcaga tagcgcgcca gtggactgaa gtccgccgat ttaccatcgc cgcccagcaa
    13381 caaatgcagc gtgccgtcta cctgcaagcc attcaacgcc gcttccgtac tgccgacgtt
    13441 agtggctttc gaatcgttaa tccagcgtac gccattgtgc tccagcacca cttcaaagcg
    13501 atgcggcaga ccggtgaagg tggttaacgc tttcaggctg ctggcgcgcg gcaaaccagc
    13561 tgcgtctgcc agtgccagtg ctgccagcgc attggtgtag ttatgctgcc cggaaagttt
    13621 catctctttc acgttcagca ctttctcgcc tttcactcgc agccaggttt ctccctgctg
    13681 atgattcagg tgatagtcac ccatgttgac gccaaagctg acgcagcgtt catcagcacc
    13741 gcgaatcggc attgttaagg catcatcagc attgacgaca caaactttcg cattttcgta
    13801 aatacgcaat tttgccgcac ggtattgctg caaaccaaac ggatagcggt ccatatgatc
    13861 ttcagtcacg ttcagaatgg tcgccgccac cgcctgtaag ctggaagtgg tttccagctg
    13921 gaagctcgac agttccagca cgtacagttc gcactcagca tcaagcagca tcaacgctgg
    13981 caggccaata ttgccaccca caccaacgtt aacccccgcc gctttcgcca tttcacccac
    14041 tagcgtggtg accgtgcttt tgccgttaga accggtaatc gccacaatcg gtgcttgtgc
    14101 ttcgcggcag aacagctcga tatcgccaac gatttcgatt ccggcatcag cggcagcgct
    14161 taaggatgga tgcgccagtg caataccggg actggcgaca atcagatctg ccgccatcag
    14221 ccattcatca ttcagactgc ccgtgtggcg ttctacggct tcgggtaatt tatccaggcc
    14281 aggcggtgtc atacgcgtat ccataacgcg cggcgtcaca ccgcgagcga ggaaaaagtc
    14341 cacgcaggaa agcccagtga ggcccaggcc gataatgacg acatttttac cctgataatc
    14401 agccatgatt aacgtacctt cagcgttgcc agaccaatca gaaccagcat cagcgaaata
    14461 atccagaaac gcacaatgac gcgcggttcc ggccagcctt tcagttcata gtggtgatga
    14521 atcggtgcca tgcggaaaat acgttgtccg cgcagtttaa aggagccgac ctgcaggatg
    14581 acagaaagcg tttctaccac gaacacgccc cccataatca ccagcaggaa ttcctgacgt
    14641 agcagtacgg cgataatgcc taacgcacca cctaacgcca gcgaacctac atcgcccata
    14701 aagacctgcg ccggataggt gttaaaccac aggaagccca gtcctgcccc gactatcgcc
    14761 gtgcagacaa tgaccagttc cccggcgtgt cgcagatacg gtatgtgtaa atagctggca
    14821 aagttcatgt tgccggtcgc ccacgccacc agcgcaaaac caccggcgac aaatacggtc
    14881 ggcataattg ccaggccgtc gagaccatcg gtcaggttta ccgcgttgcc agtacccaca
    14941 atgacgaagt aagccagcag aatgtagaac agccccagct gcggcatcac atctttaaag
    15001 aatgggacca ccagctgcgt tgcgggcgtg tctttgccgg caaggtacag ggcgaaggcg
    15061 atacccagcg caatgaccga catccagaaa tacttccaac gagcgatcaa ccctttggtg
    15121 tctttacgca ccactttgcg ataatcatca acaaagccaa taacaccgta acctaccagc
    15181 accaccaaca cgcaccagac gtacggattg gacgggtaag cccacagcag tacggagatc
    15241 acaatcgccg tcaggatcat aatcccgccc atggtcggcg taccgcgctt gctgaagtgt
    15301 gattcaggac cgtcgttacg caccacctga ccaaaggaaa gtttttgcaa atgagcaatc
    15361 atacgcgggc ccatccacaa tgagatgaac agcgcggtca gcaggctgac gatggcgcga
    15421 aacgtcagat aggaaaagac gttaaagccg gaataatatt tgaccaaatg ttcggccagc
    15481 caaactaaca tgtcccattc tcctgtaaag cgcgtactac ctcttccatg gcggcactac
    15541 gtgaaccctt aactaaaatc gtaattacct gttgctcagc aatcagtgat ttaagacgcg
    15601 taattaacgc agttttatca gcaaaatgtt cgccaacgcc gctggcggtg ctgatagcat
    15661 ggctttgttt acccacgctt aacacgcggt caataccagc agcttttgcc gcctcgccca
    15721 cctgtacatg gcaggcttcg ctttcagcgc ccagttccgc catatcgccc accaccagca
    15781 cgcggtagcc cggcatttca gccagtacct gaacagcggc ggtcattgaa ccgacattgg
    15841 cgttgtagga gtcgtcgagc agcaactggt tttctgccag ttggatgggg aacagacggc
    15901 ctggaacagc tttcagattt gccagccccg ctttgatagc atcaagcgtt gcgcccacgg
    15961 acatggagag cgcagcggct gccagcgcat tcgcaatatt gtgacgcccc ggcaacggca
    16021 gcagaacatc gacgctaccg gttggggttt gtagggtaaa ttccgtaccg tgcgaggtca
    16081 catggatatt ggtggcggtg aaatcgctgt tggcggcatt gggtgagaaa cgccacactt
    16141 tgcgtgagcc aattacgctc tgccagttca gccagtcgtt gttgtcggcg ttcataatgg
    16201 cgataccgtt ttccggcagg ccgctaaaga tttcaccttt cgctttcgcg acacccgcaa
    16261 gcgagccaaa accttccaga tgcgccgctg ccaggttgtt gaccagcgca gcttccgggc
    16321 gagtcagact cacagtccag gctatttcgc cctgatggtt cgcgccaagt tcaataactg
    16381 cgtaatcgta ttccggcgtt aagcgcaaca gcgtcatcgg tacaccgatg tcgttgttga
    16441 gattgcctgc cgtataaagc gtgttgccgc actggcttaa aatcgccgcc gtcatctctt
    16501 taacggaggt tttgccggag gaccccgtca gagcaaccac gcgcgccgga acttgctggc
    16561 gaacccatgc agccagttca ccaaacgcca gacgcgtatc cttgacgatt aactgcggca
    16621 ggtcgatgtc cagcggacgg ctaaccagta gtgcgcctgc gccgccagct ttcgcctggt
    16681 cggcaaaatc gtgggcatca aaacgttcgc ctttcagggc aacaaacagg cagcccggcg
    16741 tcagttttcg ggtatcagtg gttacagcat caagggtgat atctgcacct tgcagttcac
    16801 cgttgagaat atcggtaagt tggctaaggg ttacgctaat catgcaatca cccccagcag
    16861 acgcgccacc gtgacgcgat cggagtagtc cagacgctga ttgccaacaa tctggtaatc
    16921 ctcatggcct ttgcccgcga ccagtaccac atcattctct ttagcctgca taacggcgca
    16981 agtcaccgct tcagcacggc cttccatcac tttggcatgt ccggcatcta acattcccgc
    17041 cagaatatcg ttgatgatgg cacgcggttc ttcggtacgc gggttatcgt ccgtcaccac
    17101 cgccacgtca gcaaactctt cggcaattgc gcccatcagt ggacgcttac ctttatcgcg
    17161 atcgccacca cagccaaaga cgcaccacag cttgcccgca cagtgcaggc gcgccgcctg
    17221 taaggctttt tccagtgcat ccggcgtatg cgcgtaatcc accaccaccg tcggtttgcc
    17281 tggcgcagtg aacacttcca tacgtccgca aaccggttgc agacgcgcgg cggtttttaa
    17341 cagatcagcc agcgggtagc caagtgccag cagtgtcgcc agcgcgagca gcaggttgct
    17401 gacgttaaaa gcgcccatca gatggctttc aatttcgcca tcgccccaac ttgaactaaa
    17461 gcgaatggtc gcgccgctgt cgtgatagtt cacttcggtc gctttcaacc agcgtccgtg
    17521 acagttcgga ttgatatgat cttccattga taccgcaacc gcgtccggca gttttgccag
    17581 ccagcggcgg cccacttcat cgtcggcgtt aacaatcgcc tgaccacaat gatgttcaga
    17641 ataaagcagc catttcgcgg cttcgtagtg ttccatatca ccatgataat caaggtgatc
    17701 gcggcttaag ttggtaaaga ccgacgccgc aaatttcaat gccgccacac ggtgctgtac
    17761 cagcccgtgg gaggaaactt ccattgcgca aaacgtcgcg ccctgatcca ccagccccgc
    17821 cagctcatgc tgaacatcga ctgccgaacc ggtggtattt tctgtcggga tcactttccc
    17881 cagcagaccg ttaccaacgg tgcccattac cgcgctggtt tcgccaagca gttggctcca
    17941 ctgcgccagc agctgggtag tcgtggtttt gccgttggtg cccgttacgc ctacgagacg
    18001 taaattgtca gagggttcat ggtaaaagcg gcccgccagt gcagataaac gctcgttgag
    18061 ctggctgaga tagatgaccg gtacgccgtg catttcacgg atttcaccgt cggtcgcctc
    18121 acctttcgcc tctgcaataa tggcagtcac accttgcgct atcgcctgcg ggatatatcg
    18181 acgcccgtcc gcctgatgac ctactacagc tacaaagaga tcgcccgccg cagccacacg
    18241 gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg tctggcaccc acggagcaag
    18301 aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc ttgattaatc acaaattcat
    18361 ttttatcgcc cgttgtcagc gcatccggct cgatgttcat ggtacgcaat acgccgccca
    18421 tgatggcacc aaagaccggc gctgaaacgg cgccgccgta gtatttaccc gcctgcggat
    18481 cgttgataac aacaaccagc gcgaagcgcg gctgactcgc aggcgcaacg cctgcggtat
    18541 aagcaatata tttattgatg tagcgaccgt ccggcccgac ctttttcgcg gtaccggttt
    18601 taatggcgat acgatagcct ttaatcgccg ccttcacgcc gccgccgcct ggtagcgcca
    18661 cgctttccat catatgcacc acagtgcgga caatggattc cgggaagaca cgttcaccgg
    18721 gaaccggggg gtcaactttg gtaatcgaca gtgggcgata aatgccgtag ctgccgatag
    18781 ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag cccgtagccg aaagagaagg
    18841 tggccctctc tatgtcagac caccgttgtt tttgaggata taagccactg cgttctccga
    18901 ccaaccccaa attggtcgct tttcccagtc caaaacgtga gtaagtatct actaacgctg
    18961 aggacggcat cgctaacgcc agcttggaaa caccgacgtt actcgacttc tgtaataccc
    19021 cggtcagggt taattcgctg tagcgtgcca cgtctttgat ttcgtggccg ttaattcgat
    19081 aaggaatggt attgagtacc gagttttccc gcaccacgcc acgttgcaac gcggtcatta
    19141 ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc ggtgatggta cggttacgca
    19201 tcgcctcttt cggcgtgccg ctcagattgt tagggttgta tgacgggctg ttagccatcg
    19261 ccagcacttc accggtgttg acatccacca gcacggcgct accagattca gccttgttaa
    19321 aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg caggcgttca tcaatactca
    19381 gcgccaggtt gtgcgctgcc tggctgtcag tagaagaaat atcttcaatt acgcgaccat
    19441 agcggtcttt acgcacaatg cgctcacccg gctgcccggt aagccattta tcgaaactct
    19501 tctcaacgcc ctcaatccct tgactatcga cgttagtaaa gccgatgagg tgagcagtca
    19561 cttcgccgga cggatagtaa cggcgagact cttcacgcag atgaatcccc ggcagtttca
    19621 gttttttgat gtagtccgcc atgtcagggt tcacctgacg cgccagataa ataaagcgcc
    19681 ctttcgggtt ggcgttaatg cgggctgaaa gctgatccag cggaatattg agcgcgttag
    19741 ccagcgcctt ccagcggtca ccgacgctga taccgccagc gtcatgcact tctttcgggt
    19801 cagcccaaat cgcttttacc ggcacgctca ccgctaacgg gcgaccagaa cggtcagtaa
    19861 tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga acgcatgtcg ccctctttca
    19921 ccagcatatc cggggagata acttgtaacc acgctacgcg tccgagcaga aaagccagcg
    19981 ccaggagaat acagccgcat aacaacgcaa aacgccaact gataaagttg gcatgttctt
    20041 cctgacgttt tggtttctgc gttttcgccg ctgctttcat gcgtcgcgtt tatccttatt
    20101 tttgcactac gatattttct tgtgacggat caacatgctg catttgcagc ttttccgtgg
    20161 cgatcctttc cacccggcta tggtcgccga gcgcattctc ttcaaggatc aggttgcgcc
    20221 attcaatgtc taaagcatct cgctccagca ccagttgttc gcgctgagcg gtcagtaaac
    20281 gggtatggtg cgccgtggtt accacagtca ccgccgtcaa aataatgcaa atgaacaggc
    20341 agagtggcag cttcccaaat cgcaaaagat cgtcaccgat aacaccaggc aatgcatggc
    20401 gctcgtggct tcccatcgat cctttaactt tgcttagagc ttctgtcact ctgctgatca
    20461 tgcattcgtc ctctctgcaa tacgcagaac tgaactacgg gcacgagggt tctcagccac
    20521 ctcttcttcg cccggcatta acttgcctag tgctcgcagc tgacggccac ccagtttttt
    20581 gagctgctct tcagtcatcg gtaaccctgc cggaacttgc ggaccgcggc tgttttcacg
    20641 cataaaacgt ttcacaatac ggtcttccag cgagtggaag ctgatgatcg aaagccgccc
    20701 acccggggcc agcacgttga gcgagctttt tagcgcctgc tctatctcct ccagttcact
    20761 gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg ggatgtttaa acttatcttt
    20821 caccggcgtt gcagcagcca cgacttccgc cagttctttg gtgcgggtca tcggctgttc
    20881 gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca aaacgctctt caccataggt
    20941 tttcaatacc caggcgatat cggcttcttc tgcggtttgt agccattcag cggctgactg
    21001 cccacgggtt gggtccatac gcatgtccag cggaccatcg cgcataaagg aaaagccgcg
    21061 ctcagcatca tcaagttgcg gtgaagagac gccaagatcg aggagaatgc cgtcgatctt
    21121 gccgataaga tcgcgctcgg caacgtattc gcccagcgcg gagaaaggtc cgtggatgat
    21181 ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata gcctgcgggt cgcgatcgat
    21241 cgccagcaaa cgcccctctt cgccaagctg cgagaggatc agacgtgagt gaccaccgcg
    21301 accaaaagtc ccatcaatgt agatgccatc aggacggata ttgaggccat taacggcttc
    21361 atccagcagc accgtagtat gtttatagtt ttccatcatt ttatagagac aagtcctgca
    21421 gtcgctccga taagtctccg gtagccaact gctctgcgtc gatatcttcc ttgacctgtt
    21481 gatgccaggt tgtttcatcc cacagctcaa acttgttgaa ctgtccaacc agcatcactt
    21541 cttttgtcag cccggcatgt tgccgcagta ctggcgcgat taacaatcga cctgcgccat
    21601 ccatctgaca ttcgctggca tgacctaaca gtaggcgctg cacacggcgc tcaaccgggt
    21661 tcatgctcga cagacgcgat aatttttgct cgataatttc ccattcaggt agggggtaaa
    21721 gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat ttgaccggca gcgttctcaa
    21781 gcagctgttc ccggtaacgg gtaggcactg ataagcgccc tttgctgtcg agattgacta
    21841 acgttgctcc ccggaacatg ccagcctcac cccttattcc cactttgccc cacaatttcc
    21901 cactgaaagg agtttacgga gcggaggaaa agcttgtcaa gccagtcaca aggcataccg
    21961 aggtaaaaaa cccgatgttt acggctaata tcagcgttgt ttattttacg tccaacgtgc
    22021 gcagcaaatt aacgttatga atagctgtaa gaaaaaatcc ctcatcgtca tcaccgtttt
    22081 aaaatgactc actattttcc acagcaaata taaagtgtca gtttgcgacg cgagcggcat
    22141 tttaggacat atcttccccg gttaacagtc cctgttgcgt ctgtgtggcg cttgatgcca
    22201 gcgggtttgc gcaaaagcaa agaaaagtgc ttgttaaatc ggcaattcat ctctgcatgt
    22261 aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat tattgtgaat ttcagctggt
    22321 gagttaatta acgggcgggt ttaaaagaac ttatcaataa tagtcaggaa tagtcttatt
    22381 tactttaagg atattgatgt ccattcccgt actctacgcg ccagagggaa attcacctgg
    22441 cgcgtatttt tgttcgcggc ttagctacgg ctgagcacac cgcggcgata gagattacgt
    22501 ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt ccaggcttgc caggacaatc
    22561 tccagcacac gctctgcgac atcgcggtga cgttgagcca ctgccagcac cgggcactgt
    22621 aagaagtcga gcagttcgtt atcgccaaag gtggcaattg ccaggtcaga aggcagttta
    22681 ccatcgcgac gtagcgtgac atccatcacc ccttgcaaca acgcaaacga cgttgtgaac
    22741 agcgcctgcg gcatcggatg cgtttccagc catttttcga ataactgggc agccgcctcc
    22801 cgctcatagc tgttggcata caggaaatgc acttcgcgcg gatcatcttt ccaggcagta
    22861 cggaaacctt gttcacgcag gaagctgaca gaaagctccg gcagcgcgcc aagataaagc
    22921 accgtctcgg cgggaaactt acgtaactct tccgccagca tttcggcatc atcctgatcg
    22981 gcaccaacca cgctggtgaa atgttcacga tcgagggcgc ggtccagcgc gacaatcggg
    23041 aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag gtggcaacga cgtcgaaaca
    23101 ataatggcat caacctgacg ctgtaaaagg tgctcaatgc agcgcatttc gttgtctggc
    23161 tgatcttctg agcaggcaat cagcagttga taaccccgtt gccgcgcctg gcgctcaaga
    23221 tagttagcga tgcgggtata gctggtgttc tccagatcgg ggatcacaag accaatagaa
    23281 cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg ggtggtaatt gtgctcacgc
    23341 accacagcca tgactttttc aacggttttg tcgctcacac ggtattgctt cgctttgccg
    23401 ttaataacat agcttgcagt ggtccgcgac actcccgcca gccgagcgat ttcatccagt
    23461 ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc attgtactgg tatgggttaa
    23521 attcattaac atctaagcgc agaaaattaa ctgcggcaac tgcttttatt ccaggttccc
    23581 actgatttcg caaaaaaagc ccaacgtgac atgttgggct gcaaattgcg cactgagatc
    23641 attctcaacg cattatttta tcgccgcgcg aaagtccgac cacaccagag cgagcaactt
    23701 ccacaatttt cgccacatcg cgaatcgatg ctaaaaatgc atcaagctta tcgctggtgc
    23761 ctgctaattg aacggtataa agcgaggggg tgacatcgat aatttgccca cggaatattt
    23821 ccgtattacg tttcacttcg tcacgcccgt aaccgctggc ctgaattttc accagcatga
    23881 tttcccgctc aacatgcgcg ccctgcccca actcactcac gcgcaagaca tcgaccagct
    23941 tgtgtaattg cttttcgatc tgctcaagca ctttttcatc gcccacggtc tggatggtca
    24001 tacgcgataa tgtcggatcg tcggttggcg caacggtcag gctttcaatg ttgtagccac
    24061 gctgggaaaa aaggccaatc acgcgggata acgcgcctga ttcattttcg agtaagactg
    24121 ataatatccg gcgcataatc aggttctctc cgttttgctt aaccacattt catccattcc
    24181 gcccccgcga atctgcatcg ggtagacgtg ctcactgcca tcgacggtaa catcaacaaa
    24241 caccaggcga ttattgcaca cctgttccag cgcctcgcta agtttgcttt ccagctcatg
    24301 cggatgagag atctggatcc cgacgtgacc ataggcttcc gccagacgga cgaaatcggg
    24361 tagtgattgc atataagatt gtgaatgacg gccggaatag atcatgtcct gccactgttt
    24421 caccatcccc agatagcggt tattgagatt caccaccagt accggcaact cgtattgcaa
    24481 cgcggtagac agttcctgga tgttcatctg aatactgccg tcgccagtga cgcaaaccac
    24541 ggtttcttct ggcaacgcca ttttgacgcc cagtgccgca ggtaaaccaa aacccatcgt
    24601 gccgaggcca ccggaattga tccagcgacg cggtttgtcg aatggataat aaagtgcagc
    24661 aaacatctgg tgctgcccga catcggacgt cacgtaagcg tctcccttcg tcaaccgcca
    24721 aagagtctcg atcaccgcct gcggtttaat cttttcactg tgagtgtcat atttcaggca
    24781 ctgacgagcg cgccactgtt caatttgctg ccaccagtcg cggatctcat ccagtggttg
    24841 atgggcggat tcttgcgaca agagttcaag catttgttcg aggacctggc gagcatcccc
    24901 cacaatcggg atatccgcag tcacggtttt agaaatggaa gtaggatcaa tatcgatatg
    24961 cagaacagtg gcatttgggc agtactttgc cagattgttc gtcgttcggt catcaaatcg
    25021 taccccgacg gcgaaaatca catccgcgtt atgcatcgtc atattggctt cgtaggtacc
    25081 gtgcattccc agcatgccca gtgcctgacg atgcgttgcc ggaaacgccc ccagccccat
    25141 caatgaggaa acaacgggca gattcaacgc ctccaccgtt tctttcaact gctgatggca
    25201 gcccgccatg attgccccac cgcctacgta gacaaccggt ttttttgccg ctaccagcgt
    25261 ttgcagagcg cgcttaattt gccctttatg tccggtagta gtgggattgt aagaacgcat
    25321 actgaccgac tcaggccaga catagggtaa tttgttcgcc ggattaagaa tatctttcgg
    25381 taaatcaacg accactggtc ctgggcgacc acttgccgcc agccagaaag cctttttcag
    25441 cacctgcgga atgtcttccg tttgcttaac cagaaaactg tgtttaacca ccggtcgcga
    25501 aatccccacc atgtcgcact cctgaaaggc atcgtaacct atcaacgagg tcgctacctg
    25561 cccggaaagg acaactaatg gaatggaatc catataagcg gtggcgatgc cggtaatcgc
    25621 attggtcgcc cctggacccg acgttaccag cacgacgccg acttccccgg tcgcgcgcgc
    25681 cagaccatcg gccatatgca ctgccgcctg ctcatgacga actaaaacat gatcgatacc
    25741 acccacggta tgcaatgcat cgtaaatatc aaggactgcg cctccgggat aaccgaatac
    25801 ttgtttaacg ccctgatcga taagcgatcg gacgaccatc tcggctccag acaacatctc
    25861 catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa cacattaacc gtccgatctt
    25921 gcttaagcaa ttggcagcca gagacataaa cgcagggtaa taagaataaa caggaggaaa
    25981 ggtgaaaaga aacgataaaa tcctccatta cgaaggatta aattgataat aaatcggctg
    26041 aatcccacaa cttacagcaa tttctctatt tttttaatta atcctaaaac agagagccca
    26101 cacattcaga ataaaattct acattcaata aaaaatacag aataaaccag acattcatgc
    26161 ctgacctatt ctgcattcag ttagcgtttg caaattgaga ctaattgctc ttccatccac
    26221 tgatggcctt tatcgcgccc ggcagcttca tgccaggaga gataacaggt tctgctattt
    26281 tgttttaacg gcagcggtaa tacctgtaat tctaaggatt cagcgaactc ttcagccagc
    26341 caacgcggcg caatagcgac caaatgcgtt tgcgacacca cgctaagtac gctcatcatt
    26401 gccatgccct gatacgcgat actggcttgc ttatctaccg tgtcatacca aggttgacta
    26461 aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt cgttataaac atcatgtttc
    26521 agtaacgggc ccttaattgt tggatgattt ttgctggcta ccagcaccat ttcatcttta
    26581 aataatggca cgctggtaaa ttcaggacga tggaagtctt cataactaat cacaaactcc
    26641 gtttcctgat aacgcagctg atgttcagtg ttctgattta atgaagactt gaacataaca
    26701 tgtatatttg gcgcaatctg ctcaatgtga ttataaatct gcgaggtcag aatgctgtct
    26761 aacgggctgc aaacacaaag atgaaataca cgttcactgc tcgcgggttc aaaacctgaa
    26821 ccaggcaatt cattttgtac tagttgcaat gcctgacgaa ctgagccaaa aagttgaaat
    26881 gcgcgagcag tcggttgaat accacggcca taacgaacaa aaagctcgtc attaaacatc
    26941 accttcaggc gtgcaacagc gttactgacc gcaggttgcg acatacccag aacatgagcg
    27001 gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa cggttaataa gttgagatcg
    27061 accatgcgta gctgtggctt gcttaactcc gccgtctctg aatgatctgt ttgtacctct
    27121 ggcatactta actccactgt cacacttaac tccctttccc ttattggaat gcagagaaat
    27181 aatcctgaag aatatgattt atcatgcata taaaataaga aaaagcaaaa tgagtaaaat
    27241 tcggaaaaca taaagatacg ggttctgata aaaacagaat ccattgccgt ggaatgagtc
    27301 atttaccaca gcataataat ccataatgtt tttgttgcga aaacaatcta atcatgacta
    27361 ccgcgaatac tcaatcatct acaaaatgta ttaaatgtgc ttatttaata attaatttat
    27421 atatttaatg cattaattct taacattaat tgatcaataa tattcaccaa atcaatatca
    27481 aaaaaaaatc gcaaaacata taattcaata caaatcatca ggataggttt tgcaacgcgt
    27541 gcattttgtc ccctttttcc tcgttgatta gatgcaaaaa tttatgctga aatatgtcaa
    27601 ccgatgaaaa gcgtcggtag ttaagcagaa attaatatcg cttactttaa ccaccgcagc
    27661 acaattagct aattttacgg gtgcagaact cacgctggcg ggacgttttt attgcgtcag
    27721 ggttgacatc cgtttttgta tccagtaact ctaaaagcat atcgcattca tctggagctg
    27781 atttaatgac tcacatcgtt cgctttatcg gtctactact actaaacgca tcttctttgc
    27841 gcggtagacg agtgagcggc atccagcatt aagccagcac gcagtcaaac aaaaaacccg
    27901 cgccattgcg cgggtttttt tatgcccgaa gcgaggcgct ctaaaagaga caaggaccca
    27961 aaccatgagc cagcaagtca ttattttcga taccacattg cgcgacggtg aacaggcgtt
    28021 acaggcaagc ttgagtgtga aagaaaaact gcaaattgcg ctggcccttg agcgtatggg
    28081 tgttgacgtg atggaagtcg gtttccccgt ctcttcgccg ggcgattttg aatcggtgca
    28141 aaccatcgcc cgccaggtta aaaacagccg cgtatgtgcg ttagctcgct gcgtggaaaa
    28201 agatatcgac gtggcggccg aatccctgaa agtcgccgaa gccttccgta ttcatacctt
    28261 tattgctacc tccccaatgc acatcgccac caagctgcgc agcaccctgg acgaggtgat
    28321 cgaacgcgct atctatatgg tgaaacgcgc ccgtaattac accgatgatg ttgaattttc
    28381 ttgcgaagat gccgggcgta cacccattgc cgatctggcg cgagtggtcg aagcggcgat
    28441 taatgccggt gccaccacca tcaacattcc ggacaccgtg ggctacacca tgccgtttga
    28501 gttcgccgga atcatcagcg gcctgtatga acgcgtacct aacatcgaca aagccattat
    28561 ttccgtacat acccacgacg atttaggcct ggcggtcggc aactcactgg cggcggtaca
    28621 tgccggtgca cgccaggtgg aaggtgcaat gaacgggatc ggcgagcgtg ccggtaactg
    28681 ttcgctggaa gaagtcatca tggcgattaa agttcgtaag gatattctca acgtccacac
    28741 cgccattaat caccaggaaa tatggcgcac cagccagtta gttagccaga tttgtaatat
    28801 gccgatcccg gcaaacaaag ccattgttgg cagcggcgca ttcgcacact cctccggtat
    28861 ccaccaggat ggcgtgctga aaaaccgcga aaactacgaa atcatgacac cagaatctat
    28921 tggtctgaac caaatccagc tgaatctgac ctctcgttcg gggcgtgcgg cggtgaaaca
    28981 tcgcatggac gagatggggt ataaagaaag tgaatataat ttagacaatt tgtacgacgc
    29041 cttcctgaag ctggcggaca aaaaaggtca ggtgtttgat tacgatctgg aggcgctggc
    29101 cttcatcggt aagcagcaag aagagccgga gcatttccgt ctggattact tcagcgtgca
    29161 gtctggctct aacgatatcg ccaccgccgc cgtcaaactg gcctgtggcg aagaagtcaa
    29221 agcagaagcc gccaacggta acggtccggt cgatgccgtc tatcaggcaa ttaaccgcat
    29281 cactgactat aacgtcgaac tggtgaaata cagcctgacc gccaaaggtc acggtaaaga
    29341 tgcgctgggt caggtggata ttgtcgccaa ctacaacggt cgccgcttcc acggcgtcgg
    29401 cctggccacc gatattgtcg agtcctccgc caaagccatg gtgcacgtac tgaacaatat
    29461 ctggcgtgcc gcagaagtcg aaaaagagtt gcaacgcaaa gctcaacaca acgaaaacaa
    29521 caaggaaacc gtgtgatgtc gaagaattac catattgccg tattgccggg ggacggtatt
    29581 ggtccggaag tgatgaccca ggcgctgaaa gtgctggatg ccgtgcgcaa ccgctttgcg
    29641 atgcgcatca ccaccagcca ttacgatgta ggcggtgcag ccattgataa ccacgggcaa
    29701 ccactgccgc ctgcgacggt tgaaggttgt gagcaagccg atgccgtgct gtttggctcg
    29761 gtaggcggtc cgaaatggga acatttacca ccagaccagc aaccagaacg cggcgcgctg
    29821 ttgcctttgc gtaagcactt caaattattc agcaacctgc gcccggcaaa actgtatcag
    29881 gggctggaag cattctgtcc gctgcgtgca gacattgccg ctaacggctt cgacatcctg
    29941 tgcgtgcgcg aactgaccgg cggcatctat ttcggtcagc caaaaggccg cgaaggtagc
    30001 ggacaatatg aaaaagcgtt tgataccgag gtgtatcacc gttttgagat cgagcgtatt
    30061 gcccgcatcg cgtttgaatc tgctcgtaaa cgtcgccaca aagtcacctc aatcgacaaa
    30121 gctaacgtgc tgcaatcctc tattttatgg cgtgaaatcg tcaacgagat cgccacggaa
    30181 tacccggatg tcgaactggc gcatatgtac atcgacaacg ccaccatgca gctgattaaa
    30241 gatccatcac agtttgacgt tctgctgtgc tccaacctgt ttggcgacat tctgtctgac
    30301 gagtgcgcaa tgatcactgg ctcgatgggg atgttgcctt ccgccagcct gaacgagcaa
    30361 ggttttggtc tgtatgaacc ggcaggcggc tcagcaccag atatcgcagg caaaaacatc
    30421 gccaacccga ttgcgcaaat tctgtcgctg gcactgctgc tgcgctacag cctggatgcc
    30481 gatgatgcgg cttgcgccat tgaacgcgcc attaaccgcg cattagaaga aggcattcgc
    30541 accggggatt tagcccgtgg cgctgccgcc gttagtaccg atgaaatggg cgatatcatt
    30601 gcccgctatg tggcagaagg ggtgtaatca tggctaaaac gttatacgaa aaattattcg
    30661 acgctcacgt agtgtacgaa gccgaaaacg aaaccccgct gttatatatc gaccgccacc
    30721 tggtgcatga agtgacctca ccgcaggcgt tcgatggtct gcgcgcccac ggtcgcccgg
    30781 tacgtcagcc gggcaaaacc ttcgccacta tggatcacaa cgtctctacc cagacgaaag
    30841 acattaatgc ctgtggtgaa atggcgcgca tccagatgca ggagctgatt aaaaactgca
    30901 aagaatttgg cgtcgaactg tatgacctga atcacccgta tcaggggatc gtccacgtaa
    30961 tggggccgga acagggcgtc accttgccgg ggatgaccat tgtctgcggc gactcgcata
    31021 ccgccaccca cggcgcgttt ggcgcactgg cctttggtat cggcacttcc gaagttgaac
    31081 acgtactggc aacgcaaacc ctgaaacagg gccgcgcaaa aaccatgaaa attgaagtcc
    31141 agggcaaagc cgcgccgggc attaccgcaa aagatatcgt gctggcaatt atcggtaaaa
    31201 ccggtagcgc aggcggcacc gggcatgtgg tggagttttg cggcgaagca atccgtgatt
    31261 taagcatgga aggtcgtatg accctgtgca atatggcaat cgaaatgggc gcaaaagccg
    31321 gtctggttgc accggacgaa accaccttta actatgtcaa aggccgtttg catgcgccga
    31381 aaggcaaaga tttcgacgac gccgttgcct actggaaaac cctgcaaacc gacgaaggcg
    31441 caactttcga taccgttgtc actctgcaag cagaagaaat ttcaccgcag gtcacctggg
    31501 gcaccaatcc aggccaggtg atttccgtga acgacaatat tcccgatccg gcttcgtttg
    31561 ccgatccggt tgaacgcgcg tcggcagaaa aagcgctggc ctatatgggg ctgaaaccgg
    31621 gcattccgct gaccgaagtg gctatcgaca aagtgtttat cggttcctgt accaactcgc
    31681 gcattgaaga tttacgcgcg gcagcggaga tcgccaaagg gcgaaaagtc gcgccaggcg
    31741 tgcaggcact ggtggttccc ggctctggcc cggtaaaagc ccaggcggaa gcggaaggtc
    31801 tggataaaat ctttattgaa gccggttttg aatggcgctt gcctggctgc tcaatgtgtc
    31861 tggcgatgaa caacgaccgt ctgaatccgg gcgaacgttg tgcatccacc agcaaccgta
    31921 actttgaagg ccgccagggg cgcggcgggc gcacgcatct ggtcagcccg gcaatggctg
    31981 ccgctgctgc tgtgaccgga catttcgccg acattcgcaa cattaaataa ggagcacacc
    32041 atggcagaga aatttatcaa acacacaggc ctggtggttc cgctggatgc cgccaatgtc
    32101 gataccgatg caatcatccc gaaacagttt ttgcagaaag tgacccgtac gggttttggc
    32161 gcgcatctgt ttaacgactg gcgttttctc gatgaaaaag gccaacagcc aaacccggac
    32221 ttcgtgctga acttcccgca gtatcagggc gcttccattt tgctggcacg agaaaacttc
    32281 ggctgtggct cctcgcgtga gcacgcgccc tgggcattga ccgactacgg ttttaaagtg
    32341 gtgattgcgc cgagttttgc tgacatcttc tacggcaata gctttaacaa ccagctgctg
    32401 ccggtgaaat taagcgatgc agaagtggac gaactgtttg cgctggtgaa agccaatccg
    32461 gggatccatt tcgacgtgga tctggaagcg caagaggtga aagcgggaga gaaaacctat
    32521 cgctttacca tcgatgcctt ccgccgccac tgcatgatga acggtctgga cagtattggg
    32581 cttaccttgc agcacgacga cgccattgcc gcttatgaag caaaacaacc tgcgtttatg
    32641 aattaatccc cttgcccggt caaatgaccg ggctttccgc tatcgtccac gtcatcaaac
    32701 gtctttaacc tttgcggtta aaaataatgc gacaacagaa ataaccgcaa ttacccagta
    32761 gacagcaaag tgcccccaac tttgtgcaat tgctccctga ataacgccag ccagaattac
    32821 cccggtagaa atactgttag taaataaggt ggtagctgcc cccgctcttc caggcattaa
    32881 atcctgaaac catagcatcc caatacccgc aacaatgccg ataaatacag cgttaaaaag
    32941 ttgcagcgtc atcaacgcca tacggctatg aaagaaaatc aatccggtgt aaaacagtac
    33001 tcctgccgcc actgctatga ccatcattcg ccgcttacca taacgtttga catagtagcc
    33061 agccagaatc attgctggta tttccagtcc agctgccgtc cccatcagga aacccgccag
    33121 tttgtctggc aatcctaact cgctactgat ccacaacggc atatcaataa tgtacatggt
    33181 gttgcaggtc cacattaacg tcgaggcgac aaataacatc cgtacgttac tatcctgcca
    33241 gccaccttgc attgataaag cattttccga cggcagttct acccgcgcca cagacggaag
    33301 cataaatgca atcaatacca gactgagtgt gaatatcccg gcggcaatcg aaaacatcac
    33361 cgtaaagccg taattcaacg ccagcataaa ggccaacggt ggaccgataa cccatgccag
    33421 agaaagctgc gcacgcatca ccgagctaaa catcaccact tctcgcgccg agttatccgc
    33481 atattcccgc gccagagcaa ataactgtgg cattgccgta ttggccagag atgccagaag
    33541 cacaccacag gtgataagcg tcagataatg acgattaaat gcaaacaata gcgcattgcc
    33601 gatagccatc aaacagcaaa atataatcag ttttcgccga tcgccctgac tgtcagaacg
    33661 ttttgccaac cagaggctta cgccgatccc agcaatagca ttcaccgtat aaaagagtcc
    33721 gatccagaaa ggttgcgcgc caacctcacg actcagaaat aagctcaatg taggagcctg
    33781 tagcgccccg gccaccccca tcataaaagc gaccagcata aatgccgcgt aaacaccgtt
    33841 catacggcga gccatcgtca ttatccagat catacgttcc ctttttagcg cggcgagaat
    33901 aaaaaaaacc agcaggtata atctgctggc gggtgatttt acaccaatac tcagtcacac
    33961 atgatgcagg caagtcaact ttcagaattg cggtcatccc actgcatcag ttcttcaagc
    34021 atggttaatc gttgtgggac gcttaaccaa cgcaaccagc acaacttcgc tgtcgcggta
    34081 aaatagtgct gataaaactg acgcatgggg cacctccttg cttcatcgtt aggaattatt
    34141 gacttaatat agggaaaata aaattgctgt cttttgcgca ggagttcccc ttttatgcca
    34201 tctgctcgtc tgcaacaaca gttcatccgc ctgtggcaat gctgcgaggg taaatcgcag
    34261 gacacaacgc tcaacgaact ggcagcgtta ttgagctgct cgcgtcgtca tatgcgtacc
    34321 ctgctcaaca ccatgcagga tcgcggctgg ctgacgtggg aagcggaagt cgggcgcggt
    34381 aaacgctcgc gtctgacatt cctctatacc gggctggcgc ttcagcaaca gcgggcggaa
    34441 gacctgctgg agcaggatcg tatcgaccaa ctagtgcagt tggttggcga caaagcgact
    34501 gtgcggcaaa tgctggtttc tcatctgggc cgcagcttcc gccaggggcg gcacatcctg
    34561 cgcgtgctct actatcgtcc gttgcgtaat ctgctacctg gcagcgcatt gcgccgttcc
    34621 gaaacccata ttgcccggca aatcttcagt tcgctaacgc gcataaatga ggaaaatggg
    34681 gaactggaag cagacatcgc ccaccactgg cagcaaatat caccgcttca ctggcgtttc
    34741 tttttgcgtc caggagtcca ttttcatcat ggtcgtgaac tggaaatgga cgacgtgatc
    34801 gcctctttaa aacgaatcaa tacgctgccg ctctattcgc atattgcgga cattgtgtcg
    34861 ccgacgccct ggacgctgga tatccatctc acgcaaccgg accgctggtt accgttactg
    34921 ctgggacaag ttccggcgat gatcctgccg cgcgaatggg aaaccctcag taactttgcc
    34981 agccatccca tcggcaccgg tccgtatgcg gtgatccgca acaccaccaa tcaactgaaa
    35041 attcaggcat tcgatgactt cttcggttac cgggcattaa tcgatgaagt taacgtctgg
    35101 gttctgccgg aaattgccga cgagccagct ggaggtctga tgctaaaagg tccacagggt
    35161 gaggaaaaag agattgaaag ccgcctggag gaaggttgct actatttact gttcgacagc
    35221 cgcacccatc gcggggcgaa tcagcaagtc agggactggg taagctatgt gctttctcca
    35281 actaatctgg tctatttcgc tgaggaacag taccagcaac tgtggttccc ggcttatgga
    35341 ctgctccccc gttggcacca tgcccgcacc ataaagagcg aaaaaccggc tggcctggaa
    35401 agcctcatcc tgacctttta tcaggatcac agtgagcatc gggtgattgc cgggatcatg
    35461 cagcagattc tggcaagtca ccaggtcacg ctggaaatca aagagatcag ctacgatcag
    35521 tggcatgaag gagagatcga gagcgatatc tggcttaaca gcgccaactt tacgctgcct
    35581 ctggattttt cgctgttcgc gcacttgtgc gaggtgccgc tgctccaaca ctgtattccg
    35641 atcgactggc aagccgacgc cgcccgctgg cgcaatggcg aaatgaacct ggcgaactgg
    35701 tgccagcaac tggtcgccag caaagcaatg gtgccgctta tccaccactg gctgatcatt
    35761 caggggcaac gcagtatgcg cggcctgcgc atgaacaccc tcggctggtt cgattttaaa
    35821 tcagcgtggt ttgcgccacc ggatccatga ttgctggtag ataacaaaat cactacacta
    35881 acgccgttct caacggggtg ccacgcaaac gcgtgcgctg agaaaatacc cgtcgaacct
    35941 gatccggata acgccggcga agggatttga ggctccttct caagtccttt gccactcttt
    36001 tttgaggtgc aaagtgttaa aaaaatgtct tcccctgctg ttgctgtgca cagcgcccgt
    36061 tttcgctaaa cccgttctga ctgtttatac ctacgattcc ttcgccgccg actgggggcc
    36121 tggtccaaag atcaaaaaag cttttgaagc cgactgtaac tgcgaactga aactggtggc
    36181 gctggaagat ggcgtttcgc ttctcaaccg tctacggatg gaaggcaaaa atagtaaagc
    36241 cgatgtggtg ctggggctgg ataacaatct gttagacgcc gccagtaaaa ccggactatt
    36301 tgccaaaagc ggtgtggcag cggatgccgt taacgttccc ggcggctgga ataatgacac
    36361 tttcgtcccg tttgattacg gctatttcgc cttcgtctat gacaagaaca aactgaaaaa
    36421 cccgccgcaa agcctgaaag aattggttga gagcgatcag aactggcggg tgatttatca
    36481 ggatccgcgc accagtacgc cggggctggg cctgctgttg tggatgcaaa aagtctatgg
    36541 cgatgacgcc ccacaagcct ggcaaaaact ggcgaagaaa accgtcacgg tcaccaaagg
    36601 ctggagcgaa gcctacggcc tgtttttaaa aggtgaaagc gatctggtac tgagttacac
    36661 cacctctccg gcttatcaca ttctcgaaga gaaaaaagat aactacgccg ccgcgaactt
    36721 tagcgaaggt cactatctgc aggtggaagt cgccgcccgc accgctgcca gcaagcagcc
    36781 ggagctggcg caaaaattcc tccagtttat ggtttctccg gcttttcaga atgcgatccc
    36841 aaccggtaac tggatgtatc cggtggcaaa tgtcacgctg cctgccgggt ttgaacaatt
    36901 gaccaaaccg gccaccacgc tggagttcac accagctgaa gtggctgcac aacgtcaggc
    36961 atggattagc gaatggcaac gcgccgtcag ccgttaattc ccggctggtt aattccaggt
    37021 gtaagcgccg ccacgctggt ggtagcggtt gcgctcgcgg cgtttctcgc cctgtggtgg
    37081 aacgcgccgc agggtaactg ggtggcagtc tggcaggaca gctacctgtg gcatgtggtg
    37141 cgcttctcct tctggcaggc gtttctctcg gcgctactct ctgtcgtacc cgcgatattc
    37201 ctcgcccgcg ccctctatcg caggcgcttt ccgggtcggc tggcgctgtt gcgtctgtgc
    37261 gcaatgacct tgatcctccc ggtgctagtt gctgttttcg gcattcttag cgtctatggt
    37321 cgtcagggct ggctggcatc gctctgccaa tcgctcggtc tggagtggac cttttcgccc
    37381 tacggcctgc aaggcatttt gctggcgcac gtatttttta atctgccgat ggcgagccgc
    37441 ttattactcc aggcactgga aaacattccc ggcgaacaac gtcagcttgc cgcccagctt
    37501 gggatgcgcg gctggcattt tttccgcttc gtcgaatggc cgtggctacg gcgacaaatc
    37561 ccgccggttg ccgcgcttat ctttatgctc tgtttcgcca gcttcgccac cgtgctatcg
    37621 ctgggcggcg gtccgcaggc aactactatc gagctggcta tttatcaggc gctgagttac
    37681 gactacgatc ctgcccgcgc ggcgatgctg gcgctgctcc agatggtgtg ttgccttggg
    37741 ctggtgctgt tgagtcagcg actaagtaag gccattgcgc ccggtaccac gctgctgcaa
    37801 ggctggcgcg atccggacga tcgtctgcat agccgcattt gcgacacggt gttaatagtg
    37861 ctggcgctgc tgctgttgct gccaccgttg ctggcggtga tcgtcgatgg ggtaaatcgc
    37921 cagttgccgg aagtgctggc acaaccggtg ctgtggcagg cgctgtggac ctcgttgcgt
    37981 attgcgctgg cggcaggtgt attgtgcgta gtgctgacca tgatgctgct atggagcagt
    38041 cgcgaactgc gggcgcggca gaaaatgctg gcgggtcagg cgctggagat gagcggcatg
    38101 ttgatcctcg ccatgccggg gattgtgctg gctaccggct tctttttact gctcaacaac
    38161 actatcggcc tgccacaatc tgctgacggc attgtgattt tcaccaatgc gttaatggcg
    38221 atcccttatg cgctgaaagt actggaaaac ccgatgcgcg atatcaccgc ccgctacagt
    38281 atgttgtgtc agtcgctggg cattgaaggc tggtcgcgct taaaagtggt cgagctgcgc
    38341 gccctgaaac gtccactggc gcaggcgctg gcttttgcct gcgtgctgtc gattggtgat
    38401 tttggcgtag tggcgttgtt cggtaacgat gatttccgta ccctgccgtt ttatctctac
    38461 cagcaaattg gctcctatcg cagccaggac ggcgcggtca ccgcgttaat tctgctacta
    38521 ctctgttttc tgctgtttac cgtgattgaa aaactaccgg ggcgaaatgt taaaactgac
    38581 tgatatcacc tggctttatc accatttgcc gatgcgtttt agcttaacgg tagaacgcgg
    38641 cgagcaggtg gcaatcctcg ggccaagcgg cgcgggtaaa agtaccctgc tgaatttgat
    38701 tgccggtttt ctgacgccag ccagcggttc gctgactatc gacagcgttg atcacaccac
    38761 gacgccgccg tcacgccgcc cggtatcgat gctgtttcag gagaacaacc tgttcagcca
    38821 cctgacggtc gcgcagaaca tcgggctggg gcttaatccg ggattgaagc tgaacgcggc
    38881 gcagcaggag aaaatgcacg ctatcgcccg ccagatgggg attgataatt taatggcgcg
    38941 gttaccgggc gagctttccg gcggtcagcg acagcgcgtg gcgttagcgc gttgtctggt
    39001 gcgcgaacag ccgattttat tgctcgatga accgttctct gcgctcgatc cggcgttacg
    39061 tcaggagatg ctgacgctgg tgagcacgag ctgccagcag caaaaaatga cgctattgat
    39121 ggtgtcgcac agtgtggaag atgcggcgcg gatcgccaca cgctcggtgg tggtcgccga
    39181 cgggcgcatc gcctggcagg gtaagaccaa tgagttgttg agcggtaagg cgagtgcttc
    39241 ggcactattg gggattacgg gttagtatgc actgtcggat gcggcgtgaa cgccttatcc
    39301 gacctacggg tagtgcgttt tgtaggcctg ataagacgcg gtcagcgtcg catcaggcaa
    39361 cccgcacaag actatctcct aacccccaac cactttacgc aaaatatcga tatacaccgg
    39421 catcaacggg tggcgaatta acaccaccag cgccaccacg ccgatggcag aaattaacgg
    39481 cgtcagccac aacaaacggc cgcgcgacaa ataatgactt aaacgatcgg ttgctttacc
    39541 gctgcgccat aaccgccagc acagccagcc accaacccac aaaaataccg ccgtcgccag
    39601 cagcaaccat ttaaactcgc cgctctgcat tccggctgga atatcgatcg ccgcgcccgc
    39661 cagaatccca ggcaggaagt aaaacggtgg ccacagcagg cagccgataa tattcggcgt
    39721 aatgaatttt gccaccggca gatccagcat tcccgccacc attggcacca gcggacgcgt
    39781 cgggccaaca aagcgaccga ccagaatggt gaacatgctg tgttgatgca acgcatgttc
    39841 agttttatcg agtaatgctt tgtttttctt cagaaatgac cagcgatgca acggcttttt
    39901 aaaacgccag cccagccaga aggagatcca gtcgcccagc aggcaaccga cgatccctgc
    39961 cagccaggcg tgccagaaac ttaactcgcc gctgccaatc agcgctccca gccccgccat
    40021 cagcaccgta ccgggtagaa tcaaaccgac cagcgccagc gactccagaa aggccaccaa
    40081 caccaccgcc atcaatgaat acacggtaga ttgggtaata aagtgttcca gcaatgcttg
    40141 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
    40201 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
    40261 tttcttcaca accggctcgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
    40321 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
    40381 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
    40441 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
    40501 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
    40561 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
    40621 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
    40681 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
    40741 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
    40801 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggataacgac
    40861 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
    40921 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
    40981 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
    41041 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
    41101 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca
    41161 gacattgccg tcactgcgtc ttttactggc tcttctcgct aacccaaccg gtaaccccgc
    41221 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa
    41281 gtgtctataa tcacggcaga aaagtccaca ttggttattt gcacggcgtc acactttgct
    41341 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
    41401 tctctactgt ttctccatac ccgttttttt ggatggagtg aaacgatggc gattgcaatt
    41461 ggcctcgatt ttggcagtga ttctgtgcga gctttggcgg tggactgcgc cagcggtgaa
    41521 gagatcgcca ccagcgtaga gtggtatccc cgttggcaaa aagggcaatt ttgtgatgcc
    41581 ccgaataacc agttccgtca tcatccgcgt gactacattg agtcaatgga agcggcactg
    41641 aaaaccgtgc ttgcagagct tagcgtcgaa cagcgcgcag ctgtggtcgg gattggcgtt
    41701 gacagtaccg gctcgacgcc cgcaccgatt gatgccgacg gtaacgtgct ggcgctgcgc
    41761 ccggagtttg ccgaaaaccc gaacgcgatg ttcgtattgt ggaaagacca cactgcggtt
    41821 gaagaagcgg aagagattac ccgtttgtgc cacgcgccgg gcaacgttga ctactcccgc
    41881 tacattggcg gtatttattc cagcgaatgg ttctgggcaa aaatcctgca tgtgactcgc
    41941 caggacaccg ccgtggcgca atctgccgca tcgtggattg agctgtgcga ctgggtgcca
    42001 gctctgcttt ccggtaccac ccgcccgcag gatattcgtc gcggacgttg cagcgccggg
    42061 cataagtcgc tatggcacga aagctggggt ggcttgccgc cagccagttt ctttgatgag
    42121 ctggacccga tcctcaatcg ccatttacct tccccgctgt tcactgacac ctggactgcc
    42181 gatattccgg tgggcacctt atgcccggaa tgggcgcagc gtctcggcct gcctgaaagc
    42241 gtggtgattt ccggcggcgc gtttgactgc catatgggcg cagttggcgc aggcgcacag
    42301 cctaacgcac tggtaaaagt tatcggtact tccacctgcg acattctgat tgccgacaaa
    42361 cagagcgttg gcgagcgggc agttaaaggt atttgcggtc aggttgatgg cagcgtggta
    42421 cctggattta tcggtctgga agcaggccaa tcggcgtttg gtgatatcta cgcctggttt
    42481 ggtcgcgtac tcggctggcc gctggaacag cttgccgccc agcatccgga actgaaagcg
    42541 caaatcaacg ccagccagaa acaactgctt ccggcgctga ccgaagcatg ggccaaaaat
    42601 ccgtctctgg atcacctgcc ggtggtgctc gactggttta acggtcgtcg cacaccgaac
    42661 gctaaccaac gcctgaaagg ggtgattacc gatcttaacc tcgctaccga cgctccgctg
    42721 ctgttcggcg gtttgattgc tgccaccgcc tttggcgcac gcgcaatcat ggagtgcttt
    42781 accgatcagg ggatcgccgt caataacgtg atggcgctgg gcggcatcgc gcggaaaaac
    42841 caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc cgctgcaaat tgttgcctct
    42901 gaccagtgct gtgcgctcgg tgcggcgatt tttgctgccg tcgccgcgaa agtgcacgca
    42961 gacatcccat cagcccagca aaaaatggcc agtgcggtag agaaaaccct gcaaccgcgc
    43021 agcgaacagg cacaacgctt tgaacagctt tatcgccgct atcagcaatg ggcgatgagc
    43081 gccgaacaac actatcttcc aacttccgcc ccggcacagg ctgcccaggc cgttgcgact
    43141 ctataaggac acgataatga cgatttttga taattatgaa gtgtggtttg tcattggcag
    43201 ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc caacatgccg agcacgttgt
    43261 taatgcgctg aatacggaag cgaaactgcc ctgcaaacta gtgttgaaac cgctgggcac
    43321 cacgccggat gaaatcaccg ctatttgccg cgacgcgaat tacgacgatc gttgcgctgg
    43381 tctggtggtg tggctgcaca ctttctcccc ggccaaaatg tggatcaacg gcctgaccat
    43441 gctcaacaaa ccgttgctgc aattccacac ccagtttaac gcggcgctgc cgtgggacag
    43501 tatcgatatg gactttatga acctgaacca gactgcacat ggcggtcgcg agttcggctt
    43561 cattggcgcg cgtatgcgtc agcaacatgc cgtggttacc ggtcactggc aggataaaca
    43621 agcccatgag cgtatcggct cctggatgcg ccaggcggtc tctaaacagg atacccgtca
    43681 tctgaaagtc tgccgttttg gcgataacat gcgtgaagta gcggtcaccg atggtgataa
    43741 agttgccgca cagatcaagt tcggtttctc cgtcaatacc tgggcggttg gcgatctggt
    43801 gcaggtggtg aactccatca gcgacggcga tgttaacgcg ctggtcgatg agtacgaaag
    43861 ctgctacacc atgacgcctg ccacacaaat ccacggcgaa aaacgacaga acgtgctgga
    43921 agcggcgcgt attgagctgg ggatgaagcg tttcctggaa caaggtggct tccacgcgtt
    43981 caccaccacc tttgaagatt tgcacggtct gaaacagctt ccaggtctgg ccgtacagcg
    44041 tctgatgcag cagggttacg gctttgcggg cgaaggcgac tggaaaaccg ccgccctgct
    44101 tcgcatcatg aaggtgatgt caaccggtct gcagggcggc acctccttta tggaggacta
    44161 cacctatcac ttcgagaaag gtaatgacct ggtgctcggc tcccatatgc tggaagtctg
    44221 cccgtcgatt gccgtagaag agaaaccgat cctcgacgtt cagcatctcg gtattggtgg
    44281 taaggacgat cctgcccgac tgatcttcaa tacccaaacc ggtccagcga ttgtcgccag
    44341 cctgattgat ctcggcgatc gttaccgtct gctggttaac tgtatcgaca cggtgaaaac
    44401 accgcactcc ctgccgaaac tgccggtggc gaatgcgctg tggaaagcgc aaccggatct
    44461 gccaactgct tccgaagcgt ggatccttgc tggtggcgcg caccataccg tcttcagcca
    44521 tgcgctgaac ctcaacgata tgcgccagtt cgccgagatg cacgacattg aaatcacggt
    44581 gattgataac gacacccgcc tgccagcgtt taaagacgca ctgcgctgga acgaagtgta
    44641 ttacggattt cgtcgctaag tagccacatc aggtatgtaa cgcctgatgc gacgctgacg
    44701 cgtcttatct ggcctacacg ctgcgatttt gtaggccgga taagcaaagc gcatccggca
    44761 ttgttttccc cctcgcccct ttggggagag ggccggggtg aggggaaacg acctctcagg
    44821 agtcaacatg ttagaagatc tcaaacgcca ggtattagaa gccaacctgg cgctgccaaa
    44881 acacaacctg gtcacgctca catggggcaa cgtcagcgcc gttgatcgcg agcgcggcgt
    44941 ctttgtgatc aaaccttccg gcgtcgatta cagcgtcatg accgctgacg atatggtcgt
    45001 ggttagcatc gaaaccggtg aagtggttga aggtacgaaa aagccctcct ccgacacgcc
    45061 aactcaccgg ctgctctatc aggcattccc ctccattggc ggcattgtgc atacgcactc
    45121 gcgccacgcc accatctggg cgcaggcggg tcagtcgatt ccagcaaccg gcaccaccca
    45181 cgccgactat ttctacggca ccattccctg cacccgcaaa atgaccgacg cagaaatcaa
    45241 cggcgaatat gagtgggaaa ccggtaacgt catcgtagaa acctttgaaa aacagggtat
    45301 cgatgcagcg caaatgcccg gcgttctggt ccattcccac ggcccgtttg catggggcaa
    45361 aaatgccgaa gatgcggtgc ataacgccat cgtgctggaa gaggtcgctt atatggagat
    45421 attctgccgt cagttagcgc cgcagttacc ggatatgcag caaacgctgc tggataaaca
    45481 ctatctgcgt aagcatggcg cgaaggcata ttacgggcag taatgactgt ataaaaccac
    45541 agccaatcaa acgaaaccag gctatactca agcctggttt tttgatggat tttcagcgtg
    45601 gcgcaggcag gttttatctt aacccgacac tggcgggaca ccccgcaagg gacagaagtc
    45661 tccttctggc tggcgacgga caacgggccg ttgcaggtta cgcttgcacc gcaagagtcc
    45721 gtggcgttta ttcccgccga tcaggttccc cgcgctcagc atattttgca gggtgaacaa
    45781 ggctttcgcc tgacaccgct ggcgttaaag gattttcacc gccagccggt gtatggcctt
    45841 tactgtcgcg cccatcgcca attgatgaat tacgaaaagc gcctgcgtga aggtggcgtt
    45901 accgtctacg aggccgatgt gcgcccgcca gaacgctatc tgatggagcg gtttatcacc
    45961 tcaccggtgt gggtcgaggg tgatatgcac aatggcacta tcgttaatgc ccgtctgaaa
    46021 ccgcatcccg actatcgtcc gccgctcaag tgggtttcta tagatattga aaccacccgc
    46081 cacggtgagc tgtactgcat cggcctggaa ggctgcgggc agcgcatcgt ttatatgctg
    46141 gggccggaga atggcgacgc ctcctcgctt gatttcgaac tggaatacgt cgccagccgc
    46201 ccgcagttgc tggaaaaact caacgcctgg tttgccaact acgatcctga tgtgatcatc
    46261 ggttggaacg tggtgcagtt cgatctgcga atgctgcaaa aacatgccga gcgttaccgt
    46321 cttccgctgc gtcttgggcg cgataatagc gagctggagt ggcgcgagca cggctttaaa
    46381 aacggcgtct tttttgccca ggctaaaggt cggctaatta tcgacggtat cgaggcgctg
    46441 aaatccgcgt tctggaattt ctcttcattc tcgctggaaa ctgtcgctca ggagctatta
    46501 ggcgaaggaa aatctatcga taacccgtgg gatcgaatgg acgaaattga ccgccgtttc
    46561 gccgaagata aacctgcgct ggcaacttat aacctgaaag attgcgagct ggtgacgcag
    46621 atcttccaca aaactgaaat catgccattt ttactcgaac gagcaacggt gaacggcctg
    46681 ccggtggatc ggcacggcgg ttcggtggca gcgtttggtc atctctattt tccgcgaatg
    46741 catcgcgctg gttatgtcgc gcctaatcta ggcgaagtgc cgccgcacgc cagccctggc
    46801 ggctacgtga tggattcacg gccagggctt tatgattcag tgctggtgct ggactataaa
    46861 agcctgtacc cgtcgatcat ccgcaccttt ctgattgatc ccgtcgggct ggttgaaggc
    46921 atggcgcagc ctgatccaga gcacagtacc gaaggttttc tcgatgcctg gttctcacgg
    46981 gagaaacatt gcctgccgga gattgtgact aacatctggc acgggcgcga tgaagccaaa
    47041 cgtcagggta ataaaccact ttcgcaggcg ctgaagatca ttatgaatgc cttttatggc
    47101 gtgcttggca ccaccgcctg ccgctttttc gatccgcgcc tggcatcgtc gatcaccatg
    47161 cgtggtcatc agatcatgcg gcaaaccaaa gcattgattg aagcgcaggg ctacgacgtt
    47221 atctacggag ataccgactc gacttttgtc tggctaaaag gtgcacattc ggaagaagag
    47281 gcggcgaaaa tcggtcgtgc actggtgcag cacgttaacg cctggtgggc ggaaacgctg
    47341 caaaaacaac ggctgaccag cgcattagaa ctggagtatg aaactcattt ctgccgtttt
    47401 cttatgccaa ccattcgcgg agctgatacc ggcagtaaaa agcgctacgc cgggctgatt
    47461 caggagggcg acaagcagcg gatggtgttt aaagggctgg aaaccgtgcg caccgactgg
    47521 acgccgctgg cccagcagtt tcagcaggag ctgtacctgc gtatcttccg caacgagcca
    47581 tatcaggagt acgtccgcca aaccatcgac aaactgatgg cgggtgaact ggatgcgcgg
    47641 ctggtttacc gtaaacgcct tcgccgtccg ctgagcgaat atcagcgtaa tgttccgcct
    47701 catgtacgcg ccgctcgcct tgccgatgaa gaaaatcaaa agcgcggtcg ccccttgcaa
    47761 tatcagaatc gcggcaccat taagtacgta tagaccacca acggcccgga gccgctggac
    47821 taccaacgtt caccactgga ttacgaacac tatctgaccc gccagctaca acccgtggcg
    47881 gagggaatac tcccttttat tgaggataat tttgctacac ttatgaccgg gcaacttggg
    47941 ctattttgag caaaaaaaag agttcgccag ataccatttt gatgcgtgac gaatgctttg
    48001 ccatccagta ccatagcgcc ctttccattc ctggacctga ataacgccac tacctcataa
    48061 gcacggtagc gggtggttat tgcctgcaat taaagatata gagccgaaca catatgcctt
    48121 ttacacttgg tcaacgctgg atcagcgata cagaaagcga attgggactt ggaaccgttg
    48181 tcgcggtgga tgcgcgaact gtcactttac ttttcccatc tactggtgaa aaccgtctgt
    48241 acgcacgcag tgattccccc gtgacccgcg tgatgttcaa ccctggtgat accattacca
    48301 gccatgacgg ctggcagatg caagtcgaag aagtaaaaga agaaaatggc ttgctgacct
    48361 atatcggtac tcgcctggat actgaagagt ccggcgtagc cctgcgtgaa gttttccttg
    48421 atagcaaact ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag attgaccgta
    48481 tggaccgctt tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag ttccgtatgc
    48541 cgtacagcgg cctgcgcggt cagcgtacca gcctgatccc gcatcagctc aacatcgctc
    48601 atgatgttgg tcgccgccac gcgccgcgcg ttctgctggc tgacgaagtg ggtttaggga
    48661 aaaccattga agccgggatg atcctgcatc agcaactgct ctctggcgct gctgaacgtg
    48721 tgctgattat cgtcccggaa accttacagc atcagtggct ggtagaaatg ctgcgccgtt
    48781 tcaacctgcg ttttgcgctg tttgatgatg agcgttatgc cgaagctcag cacgatgctt
    48841 acaacccgtt cgacaccgaa cagctggtga tttgctcgct ggattttgcc cgtcgtagca
    48901 aacagcgcct ggaacatctc tgtgaagccg aatgggacct gctggtggtc gatgaagcgc
    48961 atcacctggt gtggagcgaa gacgcgccga gccgcgaata tcaggctatt gaacaactgg
    49021 cagagcacgt gccgggcgtt ctgctgctga ccgcaacccc ggaacagttg ggaatggaaa
    49081 gccacttcgc ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt gcgcagttcg
    49141 ttgaagagca gaaaaattat cgtccggttg cggacgccgt tgccatgctg ctggcaggta
    49201 acaaactgag caatgacgaa ctgaacatgc ttggcgagat gatcggcgag caggatatcg
    49261 agccgctgtt gcaagcagca aacagcgaca gcgaagatgc ccagagcgcc cgtcaggaac
    49321 tggtttcgat gctgatggat cgccacggca ccagccgcgt gctgttccgt aacacccgta
    49381 acggtgtgaa aggattcccg aaacgcgagc tgcacaccat taagctgccg ctaccgacgc
    49441 agtatcagac ggctattaaa gtctccggca ttatgggcgc acgtaaaagt gcggaagatc
    49501 gtgctcgcga tatgctctac ccggagcgta tttatcagga atttgaaggt gataacgcca
    49561 cctggtggaa cttcgatccg cgcgttgagt ggctgatggg ctacctgacc agccatcgct
    49621 ctcagaaagt gctggtgatc tgcgccaaag ctgccactgc gctgcaactg gagcaggtac
    49681 tgcgcgaacg tgaaggtatt cgcgctgcgg tgttccacga aggtatgtcg attatcgaac
    49741 gtgaccgcgc tgccgcctgg tttgccgaag aagacaccgg cgcacaggta ctgctgtgct
    49801 cagaaatcgg ttctgaagga cgtaacttcc agttcgccag ccacatggta atgtttgacc
    49861 tgccattcaa cccggatctg ctggagcagc gtattggtcg tctggatcgt atcggtcagg
    49921 cgcacgatat tcagatccat gtgccttatc tggaaaaaac tgctcagtcg gtgctggtgc
    49981 gctggtatca cgaaggtctg gatgcattcg agcacacctg cccgaccgga cgcactattt
    50041 acgatagcgt ctacaacgat ctgattaact atctggcttc accggatcaa accgaaggct
    50101 ttgacgatct gatcaaaaac tgccgcgagc aacatgaagc gctgaaagca caactggaac
    50161 aggggcgtga tcgcctgctg gaaatccact ccaacggtgg cgaaaaagcc caggcactgg
    50221 cagaaagcat tgaagagcag gatgacgata ccaacctgat cgccttcgcc atgaacctgt
    50281 tcgatattat cggtatcaat caggacgatc gcggcgacaa catgatcgtg ctgaccccgt
    50341 ccgatcatat gctggtgccg gacttccctg gcctgtcgga agatggcatc accatcacct
    50401 ttgatcgtga agtggcgctg gcgcgtgaag atgctcagtt tattacctgg gaacatccgc
    50461 tgatccgcaa cggtctggat ctgattcttt ctggcgatac cggtagcagc acgatttcac
    50521 tgttaaaaaa caaagcgttg ccggtaggta cgctgttggt ggaactgatt tacgtggtcg
    50581 aagcccaggc gccgaagcag ttgcagctca accgcttcct gccaccgacg ccggtacgta
    50641 tgctgctgga taaaaacggc aacaacctgg cggcccaggt ggagtttgaa acctttaacc
    50701 gccagcttaa cgcggttaac cgtcacaccg gcagcaaact ggttaacgcc gtgcagcagg
    50761 atgttcacgc tatccttcag ctgggtgaag cgcagatcga gaaatctgcc cgtgcattga
    50821 ttgatgccgc acgtaacgaa gccgacgaaa aactgtctgc cgagctgtct cgtctggaag
    50881 cgctgcgcgc agtgaacccg aacattcgtg acgacgaact gaccgccatt gagagcaacc
    50941 gtcagcaggt aatggaaagc ctggatcagg caggctggcg tctggatgcc ctgcgtttga
    51001 tcgttgtaac gcatcagtaa cggagccgaa aatggggatg gaaaactaca atccaccgca
    51061 ggaaccctgg ttggttatcc tgtatcagga tgaccacatt atggtggtta acaagccgag
    51121 cggtttgttg tctgtgccgg gtcgtctgga agagcacaaa gatagcgtga tgacgcgcat
    51181 tcagcgtgat tatccgcagg cagaatcggt gcatcgtctg gatatggcta ccagcggcgt
    51241 gattgtagtg gcgctgacca aagccgcgga gcgggagtta aaacgccagt tccgcgagcg
    51301 cgagccgaaa aagcagtatg tggcccgcgt ctgggggcat ccatcccccg cagaaggtct
    51361 ggtggatctg ccgctgattt gcgactggcc aaaccgcccg aaacagaaag tctgttacga
    51421 aacgggtaaa cctgcgcaga cggaatatga agtggtggag tatgtggcgg ataacacggc
    51481 aagagtggtg ttaaaaccga ttaccgggcg ttcgcatcaa ttgcgtgtgc atatgctggc
    51541 gctgggtcat ccgattctcg gcgatcgttt ttatgcatca ccagaagcga gagcgatggc
    51601 accacgtttg ttgctacatg cagagatgtt gacgattacc catccggcgt atggcaatag
    51661 tatgacgttt aaagcgccag cggattttta atcgtcacgc tggcgcagtt cggtaggcgg
    51721 ataaggcgtt cacgccgcat ccggcaattg tgcgccgatt gcctgatgcg acgcgggccg
    51781 cgtcttatca ggcctacagt ttaaaactta tttaaaccct ttctgctgct ttatcagctc
    51841 atatgcctgc tgaatttcct gcgctttctg cttcgccatc tccatcatct caggcggcaa
    51901 acctttcgcc accagcttat cgggatggtg ttcactcatc agcttacggt aggcacgttt
    51961 gatggtggtc gcatcatccg tcggcttcac gcccagcaca ttacaggcat cttccagcgt
    52021 cgggccacgc tgcgcttgct gccagttacc accgccagat tgctgctgat aaccgccgcc
    52081 aaactgtgca ccgccctgca tcatgcgcaa aaactggtca aactgggcgc gggagatccc
    52141 taattcttca gcaatgacat acagcaccgc ccgttcattc gggtgcagtg aaccatcagc
    52201 aaacgccgcc tgaatctgga tctccagaaa catacgaatt aagtcaaaac gaccaaagca
    52261 gacactgcga aactggcgca tcttttcgcg cagcgggtaa ttatctgatt ttcccactcg
    52321 gaacgcgttt tgcgccgcag tacgggaagc gccatgaagg ttcattcggt ccatcaactg
    52381 gctggcgata tgaatatcag cctccgtgac gcgacctttg gatttggtta aatgccccat
    52441 cacttcaaaa gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg cgaaccacgc
    52501 cattttacgg ctacgggctt tatcaaacat atggccaatt aacaggccta acactacgcc
    52561 ccaaaagccg ccgcccatca gtaaggccac ggccacgcca atgatttttc cccaatactg
    52621 catatattcc ccaaatcgac acacggatat cagggctatc tcccacaata taaaggtgct
    52681 tttaccgttt tccggctgcg gtcaattgtg ggacatcgcc tataatttgc attatcatac
    52741 ctgtcattca atgccgtgcc taacaccact gacgctattc ggacaggatt aacactagcg
    52801 tagagatgac gagtacgtta gtctctgacc gtttgtcacg cgcaacgtta ccgatgatgg
    52861 aacaataaaa tcaacgtatg aaaaaacgta tccccactct cctggccacc atgattgcca
    52921 ccgcccttta tagtcaacag ggactggcag ccgacctcgc ctcacagtgc atgttgggcg
    52981 tgccaagcta tgaccgtcct ctggtacagg gcgataccaa tgacttaccc gtgactatca
    53041 atgctgacca cgcgaaaggg gactacccgg atgacgccgt gtttactggc agcgtggata
    53101 tcatgcaggg taacagccgt ctgcaggccg acgaagtgca gctccatcaa aaagaggcac
    53161 caggacaacc ggagccggta cgtaccgttg atgcgctcgg taatgtccat tacgacgata
    53221 accaggtgat cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa gataccaacg
    53281 tctgggaagg tgattaccag atggtgggtc gccagggtcg cggtaaagcg gacctgatga
    53341 aacaacgtgg cgaaaaccgc tataccattc tggataacgg tagctttacc tcctgtctgc
    53401 cgggttctga cacctggagc gtggtaggta gcgaaattat tcatgaccgc gaagaacaag
    53461 ttgcggagat ctggaacgcc cgctttaagg tgggtccggt accgatcttt tatagcccct
    53521 atttgcagtt gccggtgggt gacaaacgtc gctctggttt cttgatcccg aacgccaagt
    53581 acaccaccac caactacttt gagttctacc tgccatatta ctggaacatc gcgccaaata
    53641 tggatgccac catcacgccg cattatatgc atcgtcgtgg caacatcatg tgggagaacg
    53701 aattccgcta cctctcccag gcgggcgctg gcttgatgga actggactat ctgccttcag
    53761 ataaagtcta tgaagatgaa cacccgaacg atgacagttc acgtcgttgg ttattctact
    53821 ggaaccactc cggggtcatg gatcaggtgt ggcgtttcaa cgtcgactac accaaggtca
    53881 gcgatcctag ctacttcaat gatttcgata acaagtacgg ttccagtact gacggctacg
    53941 caacgcaaaa attcagcgtt ggctatgcgg tgcaaaactt caatgccacc gtttcaacca
    54001 agcagttcca ggttttcagc gaacagaaca ccagtagcta ctcggcagag ccgcagttag
    54061 acgttaatta ctaccagaat gatgttggtc catttgatac gcgtatttac ggccaggcag
    54121 tgcactttgt taacaccaga gacgacatgc ctgaagcaac ccgtgttcac ctggaaccga
    54181 ccatcaattt gccgctctct aataactggg gcagcatcaa taccgaagcg aagttgctgg
    54241 caacccatta tcagcaaacc aatcttgact ggtataactc cagaaacacg accaagctgg
    54301 acgaatccgt taaccgcgta atgccgcaat tcaaagttga cggcaaaatg gtctttgaac
    54361 gcgatatgga aatgctggct ccgggttata cccaaacgct ggaaccgcgc gcgcagtatt
    54421 tgtacgtgcc gtatcgcgat cagagcgaca tctataacta cgactcgtct ctgctgcaat
    54481 ctgactactc tggcctgttc cgggaccgga cttacggcgg tcttgaccgt attgcctccg
    54541 ctaaccaggt gacgaccggt gtcacatctc gcatatatga tgatgctgcc gttgaacgtt
    54601 ttaatatttc cgttggtcaa atctactatt tcacggagtc tcgcactggc gatgacaaca
    54661 taacatggga gaatgacgac aaaacgggtt cactggtgtg ggcaggcgat acttactggc
    54721 gtatctccga gcgttgggga ttgcgtggcg ggattcagta cgatacacgt ctggataacg
    54781 tagcgaccag taactccagc attgaatacc gtcgggatga agaccgtctg gtacagctga
    54841 attaccgtta cgccagcccg gaatatattc aggctacgct gcctaagtac tattccactg
    54901 ctgagcaata taagaatggt atttcgcagg taggtgctgt cgccagctgg ccaattgccg
    54961 atcgttggtc cattgttggg gcctactact acgacaccaa tgcgaacaag caagccgact
    55021 ctatgttagg tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt tacgagcgga
    55081 agctgaacgg ttgggataac gataaacaac atgcggtata tgacaacgca atcggcttta
    55141 acatcgaact tcgcggcctg agctccaact acggtctggg tacgcaagag atgctgcgtt
    55201 cgaacattct gccgtatcaa aacactttgt gatttgttga tttaccacgt aatccgcagt
    55261 gcggttaatt gaaatggaaa aagtatgaag aactggaaaa cgctgcttct cggtatcgcc
    55321 atgatcgcga ataccagttt cgctgccccc caggtagtcg ataaagtcgc agccgtcgtc
    55381 aataacggcg tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt aaaactgaac
    55441 gctgctcagg caaggcagca acttcctgat gacgcgacgc tgcgccacca aatcatggaa
    55501 cgtttgatca tggatcaaat catcctgcag atggggcaga aaatgggagt gaaaatctcc
    55561 gatgagcagc tggatcaggc gattgctaac atcgcgaaac agaacaacat gacgctggat
    55621 cagatgcgca gccgtctggc ttacgatgga ctgaactaca acacctatcg taaccagatc
    55681 cgcaaagaga tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg catcaccatc
    55741 ctgccgcagg aagtcgaatc cctggcgcag caggtgggta accaaaacga cgccagcact
    55801 gagctgaacc tgagccacat cctgatcccg ctgccggaaa acccgacctc tgatcaggtg
    55861 aacgaagcgg aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg cgctgatttc
    55921 ggtaagctgg cgattgctca ttctgccgac cagcaggcgc tgaacggcgg ccagatgggc
    55981 tggggccgta ttcaggagtt gcccgggatc ttcgcccagg cattaagcac cgcgaagaaa
    56041 ggcgacattg ttggcccgat tcgttccggc gttggcttcc atattctgaa agttaacgac
    56101 ctgcgcggcg aaagcaaaaa tatctcggtg accgaagttc atgctcgcca tattctgctg
    56161 aaaccgtcgc cgatcatgac tgacgaacag gcccgtgtga aactggaaca gattgctgct
    56221 gatatcaaga gtggtaaaac gacttttgct gccgcagcga aagagttctc tcaggatcca
    56281 ggctctgcta accagggcgg tgatctcggc tgggctacac cagatatttt cgatccggcc
    56341 ttccgtgacg ccctgactcg cctgaacaaa ggtcaaatga gtgcaccggt tcactcttca
    56401 ttcggctggc atttaatcga actgctggat acccgtaatg tcgataaaac cgacgctgcg
    56461 cagaaagatc gtgcataccg catgctgatg aaccgtaagt tctcggaaga agcagcaagc
    56521 tggatgcagg aacaacgtgc cagcgcctac gttaaaatcc tgagcaacta atggttaaaa
    56581 cccaacgtgt tgtgatcact cccggcgagc ccgccgggat tggcccggac ttagttgtcc
    56641 agcttgcaca gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc actctcctta
    56701 ccgaccgggc agcgatgctc ggtttgccgc tcaccctccg cccttattcc cccaactccc
    56761 ctgcacaacc gcaaactacg ggcacattaa cgctacttcc tgtcgcgcta cgtgaatctg
    56821 tcactgcggg gcagttagcg attgaaaatg gacattacgt ggtggagacg ctggcgcgag
    56881 cgtgtgatgg ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc gtgcataaag
    56941 gcgtcattaa cgacgcaggc attccgttta ccggtcatac cgagtttttc gaagagcgtt
    57001 cgcaggcgaa aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg gcgctggcaa
    57061 cgacgcattt accgctgcgc gatatcgcag atgctatcac ccctgcactt ttgcacgaag
    57121 tgattgctat tttgcatcac gatttgcgta caaaatttgg tattgccgaa ccgcgcattc
    57181 tggtctgcgg gctgaatccg cacgcgggcg aaggcggtca tatgggtacg gaagagatag
    57241 acaccattat tccggtgctc gacgagctgc gggcgcaggg gatgaaactc aacgggccgc
    57301 tgcctgccga taccctgttt cagccgaaat atctcgataa cgccgacgcc gtgctggcga
    57361 tgtaccacga tcagggtctt cccgtgctaa aataccaggg cttcgggcgc ggtgtgaaca
    57421 ttacgctggg cctgcccttt attcgcacat cagtggacca cggcaccgcg cttgaactgg
    57481 cgggacgtgg cgaagccgat gtcggcagtt ttattacggc gcttaatctc gccatcaaaa
    57541 tgattgttaa cacccaatga ataatcgagt ccaccagggc cacttagccc gtaaacgctt
    57601 cgggcaaaac tttctcaacg atcagttcgt gatcgacagc attgtttctg ccattaaccc
    57661 gcaaaagggc caggcaatgg tcgaaatcgg ccccggtctg gcggcattga ccgagccggt
    57721 cggcgaacgt ctggaccagc tgacggtcat cgaacttgac cgcgatctgg cggcacgtct
    57781 gcaaacgcat ccattcttag gcccgaaact gacgatttat cagcaggatg cgatgacctt
    57841 taactttggc gaactggccg agaaaatggg tcagccgctg cgtgttttcg gcaacctgcc
    57901 ttataacatc tccacgccgt tgatgttcca tctgtttagc tatactgatg ccattgccga
    57961 catgcacttt atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac cgaacagcaa
    58021 agcgtatggt cgattaagcg tcatggcgca atactattgc aacgtgatcc cggtactgga
    58081 agttccgccg tcagccttta caccaccacc caaagtggat tccgccgtcg tgcgcctggt
    58141 tcctcatgca acgatgcctc acccggttaa agatgttcgt gtgttgagcc gcatcaccac
    58201 cgaagccttt aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc tgtttagcgt
    58261 cgaggtgtta acgggaatgg ggatcgaccc ggcgatgcga gcggaaaata tctctgtcgc
    58321 gcaatattgc cagatggcga actatctggc ggagaacgcg cctttgcagg agagttaacg
    58381 atgatcaatt cgccccgagt gtgtattcag gttcaaagcg tctacattga ggcacaatct
    58441 tcacctgata atgaacgtta cgtttttgct tataccgtaa ccatacgcaa tctggggcga
    58501 gcgccagtgc agttgttggg gcgttactgg ctgatcacca atggcaatgg ccgtgaaacc
    58561 gaagtccagg gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg cgaagagtac
    58621 cagtacacca gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg tcactacgaa
    58681 atgatcgatg aaaatggcgt ccctttcagc atcgacattc ccgtattccg actcgccgtt
    58741 cccacactca ttcattaaaa gaatatggcg acatacctta ttggcgacgt tcatggttgt
    58801 tacgatgaac tgttcgcatt gctgcataaa gtagaattta cccctgggaa agataccctc
    58861 tggctgacgg gcgatctggt cgcgcgcggg ccgggttcgc tggatgttct gcgctatgtg
    58921 aaatccttag gcgacagcgt acgtctggtg ctgggcaatc acgatctgca tctgctggcg
    58981 gtatttgccg ggatcagccg caataaaccg aaagatcgcc tgacaccgct gctggaagcg
    59041 ccggatgccg acgagctgct taactggctg cgtcgccagc ctttgctgca aatcgacgaa
    59101 gagaaaaagt tggtgatggc ccacgcaggg atcacgccgc agtgggatct acagaccgcc
    59161 aaagagtgcg cgcgcgatgt agaagcggtg ctatcgagtg actcctatcc cttctttctc
    59221 gacgctatgt acggcgatat gccaaataac tggtcaccgg aattgcgggg gctgggaaga
    59281 ctgcgtttta tcaccaacgc ctttacccgt atgcgttttt gctttccaaa cggtcaactg
    59341 gatatgtaca gcaaagaatc gccggaagaa gcccctgccc cactgaaacc gtggtttgcg
    59401 attcctggcc ctgtcgctga agaatacagc atcgcctttg gtcactgggc atcgctggaa
    59461 ggcaaaggta cgccggaagg tatttacgcg ctggataccg gctgctgctg gggtgggtca
    59521 ttaacctgcc tgcgctggga agataagcag tattttgtcc agccgtcgaa ccggcataag
    59581 gatttgggtg agggagaggc ggtcgcgtct taacgaatta ccattcaggg aggaataatg
    59641 gctgactatt ttgaaatacg tgctgattac aatcagcaca ccatcacgat ttatcaggct
    59701 tacaatgatg caatcgctga cgtcgccgta cgtgacgggc gatttggcgc acctttttca
    59761 ttcaaccgca tgacctggat taaaccctct tttatgtgga tgatggagcg tagcaactgg
    59821 ggactgaaaa aagatcaaca acacattctc gcaatccgaa tcaaacgcac cttctttgat
    59881 acattgctgg aacaagcggt gctaaccacg ccggaagccc atgtgtatcc acatgcagga
    59941 atttgggaaa cgttatttgc acaagcaaat gtgtatgttc agtgggaccc agaacgcagc
    60001 ataaatggta aaaaactgga gcaccgatca ttacagcttg gtatttctcg taacttgatc
    60061 agtcaattta acgaagacgc gattgttgct attgatgatc tgacgccgct ggtgcgaaag
    60121 tgtcataacc ttttgataaa cggcaaaaca acacaggcga aatccttcct gccgccagaa
    60181 aaaatttacc cagttagtgc agcagcgaga aaggctttgg gaatgaaaaa ttcctgagac
    60241 aaatacgcac ctattaagcg gaaaatatct aagtgcgaca aatcaacacc gggcttaata
    60301 ccagagaaat tattctggaa atgattgacg aagcagatgc ccccccgttt gctgagcagc
    60361 ggacctggca tcgcaggcga atcgcttttc tatgtgattg catcagcgtt gctgtcgact
    60421 acggcttctt ctgttctgtc tctcagcagt caataatgaa cataaccaag atggctgaag
    60481 atgtatggat aacagaaagt aaaacgaccc aactcattga tatttggcaa caacttaaac
    60541 agctttatcc gccagaaact attccctacg cccagaagtt acccactaag gaattgcttg
    60601 aactgctact ttctagtcct gacgatcttc gaataattga aattcctgac atcatcggtg
    60661 atgtttttga tgaccaaacc tggattcgat tattcaaaaa acactttgct gatgttattc
    60721 agctacgatg tgatgtgtaa caatccaaaa tatatcgcca gacgatagac caaaaagaaa
    60781 gtatttagca gatactcaac attctgactt ctgtacagaa atagcagagt ttaaaaactt
    60841 aaaacttccg gtactcatca gagaaacatc cagtttcttc tgcaaattca tttaatgccg
    60901 agatggcatc ctcgtttact tcattccact gtagagtggc ctgaaggttt agacctacgc
    60961 aatgagcgcg actcacaaaa tcgcgctcac tggtaaaatc aaacgtctta tcgttcaatt
    61021 tgtagtcctg gcaaaaaatt accgccgctc cagaatctca aagcaatagc tgtgagagtt
    61081 ctgcgcatca gcatcgtgga attcgctgaa taccgattcc cagtcatccg gctcgtaatc
    61141 cgggaaatgg gtgtcgcctt ccacttctgc gtcgatatgc gtcagataca gtttctgcgc
    61201 ttttggcagg aactgctcat aaacgcgacc gccgccaatc accatgattt ctggtacgtc
    61261 accacacgcc gcgatggctt catccaccga cttcacccac gttacgcgat cgtccgtacc
    61321 cggttgactg ctgaggataa tatttttgcg tcctggcaac ggacgaccga ttgattccca
    61381 ggtatggcgg cccataatca cgggtttatt taaggtgttg cgtttaaacc aggcgagatc
    61441 ggcaggcagg ttccacggca tggcgttttc catgccgata acgcgatcta ccgctaacgc
    61501 cgcaatcaga ctgatcattg agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa
    61561 gcgtaaaccg tcgtcgactg gtgcgaggat gatgttgagg aaaattttat attctgctgg
    61621 cgagtccacg ctctctccct ggactcgccg cattacaatg aaacaaaaac aaacagttag
    61681 ctgtaaagtg tgatttacgt cactctttat taggatgagg gtttcgtttc cggttcatcc
    61741 gccatgttgc cggtatgttt accttcttcg gttccctgcc agccatgtcg ttgaattaat
    61801 gacagatgtt cgcggtcctc ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag
    61861 accgccgcgc gggctttggt gtcgttctca accattgcca tctcttccac catctgaata
    61921 ttaaagcggc ggaacacatc ggcacgttct cgcgcttcat acggccccag acctaaactt
    61981 tccagtgcca gacgcccggt tttcagcgca ccttcgaagg tttcacgctc cggcttttca
    62041 acgcctgcct gacgcaaacg aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc
    62101 aaatgcggga aatgttcttt caccatctcc gtcagttgca ggttggtttg cggatcatca
    62161 atagcattaa tcagcacttc cgctttcgcc gctccggcag attccagtaa atccatccgc
    62221 gttgcatcgc cataaaacac tttcatacca aatttacgca gcgtttcgat atggtccgga
    62281 tcgtgatcga gcaccaccat tttcaccccg ctggagagca gtaaacgtcc ggtaatctgc
    62341 ccaaaacgac cgaatccggc gataatcacg cgcggctgtt cttcgtcgat ctcatcggct
    62401 tcacgcgctt cctcagtaga agattgctca aggcgattga ggatcaccag cagaatcggc
    62461 gttgctgcca tcgacagcgc caccgccagg gtcagcgatt tcgcccactc cggctccagc
    62521 acattcgcca tctgcgccgc gccaaatacc acaaaggcaa actcactgcc ctgccctaac
    62581 aacaccgcaa accaacgacg ctgtttattt ggcacttgca acggtcgggc aatcagccac
    62641 agcatggcga ttttgatgat gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt
    62701 tcaagcagcg tgccaaagtc tatcgacatg ccaacaccga tgaaaaacag ccccaacagc
    62761 aaacctttaa atggttcgat atcgctctcc agcgcatgac ggtattccga gcttgccagc
    62821 agtacgcccg ccagaaacgc gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa
    62881 ccaaagccaa acacgaggaa taacgccacg gcactaaaca cttcccgcaa gccagagcgg
    62941 gctacaaaac gcagcgccgg acgcgtgaca tagcgcccca gcaataccac cagcaccagc
    63001 gcacccgcca cttttaacgc cgagagagca aatgcgccca tcgtcgtcga ggcactgctc
    63061 gttgccagta gcggaatcat cgccaccagc gggatcgccg cgatatcctg gaacagcagc
    63121 accgcaaagg cactgcgacc catttgcgtc accatcagat tgcgttcatt catcgcctgc
    63181 atggcaatcg ccgtagagga gagcgccagc gtcatgccga tcaattccgc gacctgccag
    63241 cgcagcccaa gtaacatgca gaacagcccc agcaggccgc cgcaaatcac catctgcaat
    63301 gcgccaccgc cgaacactgc cgcacgcagc ttccacagcc tttgtggatc gagttcgagg
    63361 ccgataataa acagcatcag caccacccca atctcggcaa agtgcagaat agattcggca
    63421 tcggtcacca gtcgcagccc ccacgggcca ataatgcagc cggcgatcag gtagccaagt
    63481 accgatccca gaccaagacg taccgcaatg ggtacaatca gcgctgccga accgagataa
    63541 atcagcgcct gaatcagcgt atggctatcc atgatgggcc tcctgccatt ccagcagacg
    63601 ttgcttatag tgacgcgcct gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg
    63661 catggcaaac ggtggcagcc agttcagtcc gcagtagatt gccgtcgcct gtagcggctg
    63721 cgacagcaca tcaaagcccg gatgcgcacc aatttcaaaa tggctttccc cgccgccggt
    63781 cgtcaccgcc cacagcaaat gtttgccatg cagcgccgtg ccgccatgac cgtaagccca
    63841 gccgtgggag aaaactttat cgatccaaag tttgaggagc ggaggaatgc tgtaccactg
    63901 catcgggtgc tgccagacga tcagatcggc gcgagacagc gcctcctgct cggcggcaat
    63961 atcgatattg aagtcaggat agagttgata aagagagcga atttcgacgc cttccagcgt
    64021 ccttgcctgt tcaagcatcc gtttattcgc atgggaatga tgcggatacg gatgcgcata
    64081 aattataaga atcataggta gcccgcgtga ttacgccttt gttttcccac aaagtgtagt
    64141 catatattca cgcggcttat agtcaataat attgattcag aaaagagaga aatctgaaca
    64201 atttacccca ttctctgccc gcggatcgtc atattgctgg tttgcgccag tgacatccct
    64261 cgagtctccg gggcaaacgc tacggaaatc aacaagccaa acagcgagat acccgccccc
    64321 attagcatcg tgttactgat accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa
    64381 acaatggtgc caatacggct taaggacata atcacgccca cggcagaggc gcggatatct
    64441 gtcgggaaga gttcattagg atagagccac tgcaaattac ccggcccgcc agagaaaaag
    64501 gcatacaccg caaaggccat cactaccagc cagatcccca tatccgggat cagccccaaa
    64561 accgccagcg ccagcgtcat catggcaaag ctgccaatca acaatggacg ccgtccggca
    64621 gtgtttaacc acagcatcgg cggaatacag ccgagcataa agaacaggct aatcaccacg
    64681 ttccccagcg ccgcgttttt gccaacaccc aatcccaaca aaccaacgat ttgcgggcca
    64741 aaggtgtaaa tggcgaacat tgggatcacc tggcaggtcc agatggcggc aacaaacaga
    64801 acaaaaggaa aatggcggcg attaaacaga tcgcgaaaac gggtttgctg cggctgctct
    64861 tcatcgaaag ccaccggttc gccaaacagt tttatcatca tctcttcgca ctcttttact
    64921 cgccctttgc gtaataacca gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc
    64981 aacaaacagg ggatcgccgc gctacccagc atccagcgcc agccgccttc cacatcataa
    65041 agccagtagc cgaccagatc ggcgcaggtc gcgccgacat accacatggc ggcgataaag
    65101 ctgatggaaa acgcccgctg acgggtactg gagaactcgg tgatcattga ggtggcgatg
    65161 ggataatctg caccgatgac aatgccgata agtacccgca tcaccaacag ttcgacgggg
    65221 gatgaaacaa acatcgtcgc caccgatatc acgccgatgg cgatgatatc aatgaggaac
    65281 attttgcgcc gtccgacttt atcggaaata tagccaaaca gcgatgtgcc aacgaacagc
    65341 ccggcgagcg ttcccgcgcc caacaagcca atccagtcag cgtccagttt tagcgccgga
    65401 gtcagttgct ccagcgccac gccaatcatt accagtacat aaccatccag aaacggacca
    65461 ccgcttcccc acagcaaaat gcggcggtga attgaggaga atttgagatc gtcaaagttt
    65521 ctggacggtt gcatgggtta ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt
    65581 atcaaaccaa catcagccgt aacggaactc cacaccaaag gtgccgcgcg ggtattccca
    65641 ctgttccagc gccgacccca gccccagaat gcgacaggtg ccgcactcca gacatccggc
    65701 gtaatcgaag cgcacactgc cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt
    65761 caccagcagc tccagggcct gtttatcggc atcggccttc acaacaatgt gcggatgctc
    65821 ttcatcgaca ttgaatttat tgacgcccag tttgacgtcc acattgacgg gagaagtcat
    65881 aaaacggtca ctcctttcat gccatccttg atcagattga tgaagcccac tttcttgccg
    65941 tggcggagga ttttcttgcg catcagttcc ggcgcactgc cgtcaatggt gaacaggtca
    66001 cgcgccacgc ccaccgccag ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag
    66061 gccggtagtt tctggtacat acgcatatcg cgcagcgggc cactctcaag atgctgacga
    66121 tattccgcga gtttttgctt actgaaatcg tcgcttttca tcgctgaaag cacggttttt
    66181 gctgcagctt ccccggcggc aatcgccaga tccataccgc gaatggtaaa accgaggttc
    66241 atacacattc cggcggcatc accggcaatc aatacgccgt caccaaccaa ctccggcagc
    66301 atgttgatgc ctgcttccgg cactacgtga gcggaatatt ccaccagctt gccgcccgcg
    66361 atcagcggtg ccactgccgg atgctgtttg aaatcttcca gcatttgcgg caccgatttt
    66421 tttgcgtcat gcagatgatg cagaccacaa accagcccca gcgacagggt attttcattg
    66481 gtataaagga agccgccgcc catcaggcca tcggtgggtg atcccgcaaa cagacaagcc
    66541 gcgccctgat taccctgcaa ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc
    66601 agttccttca cgccaaccgc cacatccgtc ggtttaacgc gttttgccat ccccagcttt
    66661 tcggcaagga tggagttcac tccatcagca aggatcaccg ttttcgcttc aatcacatcg
    66721 ccatcggctt ctacaccgac gactttgcca tcgcgctgta cgaggttatc gacgcggatc
    66781 ccggtaatta actgcgcgcc cgcttcttcg gcctgctcca tcagccaggc atcaaattta
    66841 ctgcgcaaaa cggagtaaga acgctgggat ggcgaggttt cgtcaccatt gcagtagtcc
    66901 atagtcatcg ctgacttttc cgtcataaac gcgagttttt catgggtgat caggcgttct
    66961 acgggggcgg agtcggcgaa accaggaata atgtgttcca ggctgtgggc atagagacgc
    67021 ccgccggtga cgttcttggc acctgcggaa ttgccacgct cgataactaa cacttgcgcc
    67081 ccttcgcggg cgagcaccag tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg
    67141 gcgtcaaaga tatcttcgga cataagagct ccctgtcagc ggcagggcca aaatgcagcc
    67201 ctgccagagt ggatcaacgc gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt
    67261 caccaacaat gccgtaatcc gcgtactgga agatcggcgc atttttatct ttattgatgg
    67321 cgaaaatggt ttgcgatgca ttagcaccaa ccatgtgctg gatctgcccg gagatcccca
    67381 ccgccaggta cagttcaggt ttcagcatca ggttggagat accgacatag cgttcgtgct
    67441 ccatccattt ttcgttttcc gccaccggac gagaacaggc caactccgca cctatcgcct
    67501 tgcaaagctg ttctgccagc gcaatgttct ctttgctgcc aataccgcga ccgacgctga
    67561 ccaccagacg ggctttgtcg agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc
    67621 gggtaatcgc cacagccgga gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg
    67681 cgtctggctg agccgcatcg aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg
    67741 caatgcgttc ttcgccaatc gccagaccac cgtaaaccat gtgtttcact gtcgctttac
    67801 cgtcctgtac gctgacggtg ctggcatcgt tagacaccgc cgctttaagg cgataaccca
    67861 gttttgccgc cagtaatttg ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc
    67921 cgtgctggcg aatagtgtca gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg
    67981 gtttgccgtt taatttccag acatgattag ctccgagctg gattgcctgt acgccgtcgg
    68041 catcattgag gacaaaggtg ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt
    68101 ccggcagacg agaaggggta tcgctgaata cccagacttg agaaaacgtg ttcatagcat
    68161 cccctgtaat taaatgactt tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc
    68221 gtcgccttca atcacgatgc gctgacgttc gcgctgtttc ggcgcagcaa cctgttgttc
    68281 agaccaggcg tccacggcgt taaaaccgat atccgccgcc gaccatacct ggactggctt
    68341 tttcgccgcg ccgagaatgg ctttcatcga aggaatttgt ggggagttga tatcagtgga
    68401 aacagcaaca accgcaggca gcggaatgct taaggtttcg gtttcatctt ccagttcgcg
    68461 ctcaacggtg agggtatctg cagtcaggga gataattttg ctgacgccgt taactgccgg
    68521 aatattgagg atttcgccca ccagcagacc aacctgctgg gcataaaggt cggaagaacc
    68581 atcgccacag aggatcaaat caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc
    68641 cgtttgttgc ggcagtgcct gctcgaactg gtcatcaatc accacaatca gttcatccgg
    68701 gccgcgcgat agcacatctt tacgcccttt ggcgttggtc agggctttac cgcccacact
    68761 taaggccgtc acctgcgcct ctgctgcctg ttgctttagc tggcaagccg cttcaatagc
    68821 gttcagatcg tattggctta ttttggcatc ggctttgctg aagtctaatg aaccatcagc
    68881 attattgacc gcaatatcct gttcatcagg cacgcactta tagcaagtaa taatcttcat
    68941 tgcatctcca gaaatcatga aggaaacgtc gttcatatcg gtggtgttaa ttacagatat
    69001 cacgcccttt aatttggaaa taaaacttta atcaccaata ttgaaaatgt cacacgcaga
    69061 taaatccaac tttcaatatt gttaagttcc tcaccaatat tgaaaacatc gcgtagcaaa
    69121 aag
//
LOCUS       DAZFWL020000008        66247 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFWL020000008 DAZFWL020000000
VERSION     DAZFWL020000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 66247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.008"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..258
                     /locus_tag="ACHKNP_RS02975"
                     /old_locus_tag="pgaptmp_000595"
     CDS             <1..258
                     /locus_tag="ACHKNP_RS02975"
                     /old_locus_tag="pgaptmp_000595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGU4702822.1"
                     /translation="WGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAID
                     FDARTAIPFEGERHNALDDARHQAKYVSAIWQKLIPNQADF"
     gene            320..589
                     /locus_tag="ACHKNP_RS02980"
                     /old_locus_tag="pgaptmp_000596"
     CDS             320..589
                     /locus_tag="ACHKNP_RS02980"
                     /old_locus_tag="pgaptmp_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4702823.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            558..1676
                     /locus_tag="ACHKNP_RS02985"
                     /old_locus_tag="pgaptmp_000597"
     CDS             558..1676
                     /locus_tag="ACHKNP_RS02985"
                     /old_locus_tag="pgaptmp_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU4702824.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1843..2637
                     /gene="potC"
                     /locus_tag="ACHKNP_RS02990"
                     /old_locus_tag="pgaptmp_000598"
     CDS             1843..2637
                     /gene="potC"
                     /locus_tag="ACHKNP_RS02990"
                     /old_locus_tag="pgaptmp_000598"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HGU4702825.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2634..3680
                     /gene="potD"
                     /locus_tag="ACHKNP_RS02995"
                     /old_locus_tag="pgaptmp_000599"
     CDS             2634..3680
                     /gene="potD"
                     /locus_tag="ACHKNP_RS02995"
                     /old_locus_tag="pgaptmp_000599"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HGU4702826.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            3738..4199
                     /locus_tag="ACHKNP_RS03000"
                     /old_locus_tag="pgaptmp_000600"
     CDS             3738..4199
                     /locus_tag="ACHKNP_RS03000"
                     /old_locus_tag="pgaptmp_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HGU4702827.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSCP"
     gene            4196..4984
                     /gene="ycfZ"
                     /locus_tag="ACHKNP_RS03005"
                     /old_locus_tag="pgaptmp_000601"
     CDS             4196..4984
                     /gene="ycfZ"
                     /locus_tag="ACHKNP_RS03005"
                     /old_locus_tag="pgaptmp_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU4702828.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(5123..5944)
                     /gene="cobB"
                     /locus_tag="ACHKNP_RS03010"
                     /old_locus_tag="pgaptmp_000602"
     CDS             complement(5123..5944)
                     /gene="cobB"
                     /locus_tag="ACHKNP_RS03010"
                     /old_locus_tag="pgaptmp_000602"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HGU4702829.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(5960..6871)
                     /gene="nagK"
                     /locus_tag="ACHKNP_RS03015"
                     /old_locus_tag="pgaptmp_000603"
     CDS             complement(5960..6871)
                     /gene="nagK"
                     /locus_tag="ACHKNP_RS03015"
                     /old_locus_tag="pgaptmp_000603"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGU4702830.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(6900..8144)
                     /gene="lolE"
                     /locus_tag="ACHKNP_RS03020"
                     /old_locus_tag="pgaptmp_000604"
     CDS             complement(6900..8144)
                     /gene="lolE"
                     /locus_tag="ACHKNP_RS03020"
                     /old_locus_tag="pgaptmp_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HGU4702831.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(8144..8845)
                     /gene="lolD"
                     /locus_tag="ACHKNP_RS03025"
                     /old_locus_tag="pgaptmp_000605"
     CDS             complement(8144..8845)
                     /gene="lolD"
                     /locus_tag="ACHKNP_RS03025"
                     /old_locus_tag="pgaptmp_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HGU4702832.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(8838..10037)
                     /gene="lolC"
                     /locus_tag="ACHKNP_RS03030"
                     /old_locus_tag="pgaptmp_000606"
     CDS             complement(8838..10037)
                     /gene="lolC"
                     /locus_tag="ACHKNP_RS03030"
                     /old_locus_tag="pgaptmp_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HGU4702833.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            10299..11372
                     /gene="ycfT"
                     /locus_tag="ACHKNP_RS03035"
                     /old_locus_tag="pgaptmp_000607"
     CDS             10299..11372
                     /gene="ycfT"
                     /locus_tag="ACHKNP_RS03035"
                     /old_locus_tag="pgaptmp_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGU4702834.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            11500..14946
                     /gene="mfd"
                     /locus_tag="ACHKNP_RS03040"
                     /old_locus_tag="pgaptmp_000608"
     CDS             11500..14946
                     /gene="mfd"
                     /locus_tag="ACHKNP_RS03040"
                     /old_locus_tag="pgaptmp_000608"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGU4702835.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            15090..16052
                     /gene="ldtC"
                     /locus_tag="ACHKNP_RS03045"
                     /old_locus_tag="pgaptmp_000609"
     CDS             15090..16052
                     /gene="ldtC"
                     /locus_tag="ACHKNP_RS03045"
                     /old_locus_tag="pgaptmp_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HGU4702836.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(16135..16392)
                     /gene="bhsA"
                     /locus_tag="ACHKNP_RS03050"
                     /old_locus_tag="pgaptmp_000610"
                     /gene_synonym="ycfR"
     CDS             complement(16135..16392)
                     /gene="bhsA"
                     /locus_tag="ACHKNP_RS03050"
                     /old_locus_tag="pgaptmp_000610"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HGU4702837.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            16633..17265
                     /gene="comR"
                     /locus_tag="ACHKNP_RS03055"
                     /old_locus_tag="pgaptmp_000611"
     CDS             16633..17265
                     /gene="comR"
                     /locus_tag="ACHKNP_RS03055"
                     /old_locus_tag="pgaptmp_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HGU4702838.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(17327..17866)
                     /gene="ycfJ"
                     /locus_tag="ACHKNP_RS03060"
                     /old_locus_tag="pgaptmp_000612"
     CDS             complement(17327..17866)
                     /gene="ycfJ"
                     /locus_tag="ACHKNP_RS03060"
                     /old_locus_tag="pgaptmp_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU4702839.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(18093..19397)
                     /gene="ndh"
                     /locus_tag="ACHKNP_RS03065"
                     /old_locus_tag="pgaptmp_000613"
     CDS             complement(18093..19397)
                     /gene="ndh"
                     /locus_tag="ACHKNP_RS03065"
                     /old_locus_tag="pgaptmp_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HGU4702840.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(19797..20339)
                     /gene="ycfP"
                     /locus_tag="ACHKNP_RS03070"
                     /old_locus_tag="pgaptmp_000614"
     CDS             complement(19797..20339)
                     /gene="ycfP"
                     /locus_tag="ACHKNP_RS03070"
                     /old_locus_tag="pgaptmp_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HGU4702841.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(20362..21387)
                     /gene="nagZ"
                     /locus_tag="ACHKNP_RS03075"
                     /old_locus_tag="pgaptmp_000615"
     CDS             complement(20362..21387)
                     /gene="nagZ"
                     /locus_tag="ACHKNP_RS03075"
                     /old_locus_tag="pgaptmp_000615"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGU4702842.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(21398..22222)
                     /gene="thiK"
                     /locus_tag="ACHKNP_RS03080"
                     /old_locus_tag="pgaptmp_000616"
     CDS             complement(21398..22222)
                     /gene="thiK"
                     /locus_tag="ACHKNP_RS03080"
                     /old_locus_tag="pgaptmp_000616"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HGU4702843.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(22203..22844)
                     /gene="lpoB"
                     /locus_tag="ACHKNP_RS03085"
                     /old_locus_tag="pgaptmp_000617"
     CDS             complement(22203..22844)
                     /gene="lpoB"
                     /locus_tag="ACHKNP_RS03085"
                     /old_locus_tag="pgaptmp_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HGU4702844.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(22858..23235)
                     /gene="ycfL"
                     /locus_tag="ACHKNP_RS03090"
                     /old_locus_tag="pgaptmp_000618"
     CDS             complement(22858..23235)
                     /gene="ycfL"
                     /locus_tag="ACHKNP_RS03090"
                     /old_locus_tag="pgaptmp_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HGU4702845.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(23238..23597)
                     /gene="hinT"
                     /locus_tag="ACHKNP_RS03095"
                     /old_locus_tag="pgaptmp_000619"
     CDS             complement(23238..23597)
                     /gene="hinT"
                     /locus_tag="ACHKNP_RS03095"
                     /old_locus_tag="pgaptmp_000619"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HGU4702846.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            23931..26120
                     /gene="fhuE"
                     /locus_tag="ACHKNP_RS03100"
                     /old_locus_tag="pgaptmp_000620"
     CDS             23931..26120
                     /gene="fhuE"
                     /locus_tag="ACHKNP_RS03100"
                     /old_locus_tag="pgaptmp_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HGU4702847.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQHIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLIGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HIKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(26180..27613)
                     /gene="ptsG"
                     /locus_tag="ACHKNP_RS03105"
                     /old_locus_tag="pgaptmp_000621"
     CDS             complement(26180..27613)
                     /gene="ptsG"
                     /locus_tag="ACHKNP_RS03105"
                     /old_locus_tag="pgaptmp_000621"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HGU4702848.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(27908..28705)
                     /gene="ycfH"
                     /locus_tag="ACHKNP_RS03110"
                     /old_locus_tag="pgaptmp_000622"
     CDS             complement(27908..28705)
                     /gene="ycfH"
                     /locus_tag="ACHKNP_RS03110"
                     /old_locus_tag="pgaptmp_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4702849.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(28716..29720)
                     /gene="holB"
                     /locus_tag="ACHKNP_RS03115"
                     /old_locus_tag="pgaptmp_000623"
     CDS             complement(28716..29720)
                     /gene="holB"
                     /locus_tag="ACHKNP_RS03115"
                     /old_locus_tag="pgaptmp_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGU4702850.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(29717..30358)
                     /gene="tmk"
                     /locus_tag="ACHKNP_RS03120"
                     /old_locus_tag="pgaptmp_000624"
     CDS             complement(29717..30358)
                     /gene="tmk"
                     /locus_tag="ACHKNP_RS03120"
                     /old_locus_tag="pgaptmp_000624"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4702851.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(30348..31370)
                     /gene="yceG"
                     /locus_tag="ACHKNP_RS03125"
                     /old_locus_tag="pgaptmp_000625"
     CDS             complement(30348..31370)
                     /gene="yceG"
                     /locus_tag="ACHKNP_RS03125"
                     /old_locus_tag="pgaptmp_000625"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HGU4702852.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(31373..32182)
                     /gene="pabC"
                     /locus_tag="ACHKNP_RS03130"
                     /old_locus_tag="pgaptmp_000626"
     CDS             complement(31373..32182)
                     /gene="pabC"
                     /locus_tag="ACHKNP_RS03130"
                     /old_locus_tag="pgaptmp_000626"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HGU4702853.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(32302..33543)
                     /gene="fabF"
                     /locus_tag="ACHKNP_RS03135"
                     /old_locus_tag="pgaptmp_000627"
     CDS             complement(32302..33543)
                     /gene="fabF"
                     /locus_tag="ACHKNP_RS03135"
                     /old_locus_tag="pgaptmp_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGU4702854.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(33631..33867)
                     /gene="acpP"
                     /locus_tag="ACHKNP_RS03140"
                     /old_locus_tag="pgaptmp_000628"
     CDS             complement(33631..33867)
                     /gene="acpP"
                     /locus_tag="ACHKNP_RS03140"
                     /old_locus_tag="pgaptmp_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4702855.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(34078..34812)
                     /gene="fabG"
                     /locus_tag="ACHKNP_RS03145"
                     /old_locus_tag="pgaptmp_000629"
     CDS             complement(34078..34812)
                     /gene="fabG"
                     /locus_tag="ACHKNP_RS03145"
                     /old_locus_tag="pgaptmp_000629"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HGU4702856.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNEIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(34825..35754)
                     /gene="fabD"
                     /locus_tag="ACHKNP_RS03150"
                     /old_locus_tag="pgaptmp_000630"
     CDS             complement(34825..35754)
                     /gene="fabD"
                     /locus_tag="ACHKNP_RS03150"
                     /old_locus_tag="pgaptmp_000630"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGU4702857.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLIASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVINFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(35770..36723)
                     /gene="fabH"
                     /locus_tag="ACHKNP_RS03155"
                     /old_locus_tag="pgaptmp_000631"
     CDS             complement(35770..36723)
                     /gene="fabH"
                     /locus_tag="ACHKNP_RS03155"
                     /old_locus_tag="pgaptmp_000631"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGU4702858.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(36791..37861)
                     /gene="plsX"
                     /locus_tag="ACHKNP_RS03160"
                     /old_locus_tag="pgaptmp_000632"
     CDS             complement(36791..37861)
                     /gene="plsX"
                     /locus_tag="ACHKNP_RS03160"
                     /old_locus_tag="pgaptmp_000632"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGU4702859.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(37942..38115)
                     /gene="rpmF"
                     /locus_tag="ACHKNP_RS03165"
                     /old_locus_tag="pgaptmp_000633"
     CDS             complement(37942..38115)
                     /gene="rpmF"
                     /locus_tag="ACHKNP_RS03165"
                     /old_locus_tag="pgaptmp_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGU4702860.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(38167..38688)
                     /gene="yceD"
                     /locus_tag="ACHKNP_RS03170"
                     /old_locus_tag="pgaptmp_000634"
     CDS             complement(38167..38688)
                     /gene="yceD"
                     /locus_tag="ACHKNP_RS03170"
                     /old_locus_tag="pgaptmp_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HGU4702861.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            38887..39471
                     /gene="yceF"
                     /locus_tag="ACHKNP_RS03175"
                     /old_locus_tag="pgaptmp_000635"
     CDS             38887..39471
                     /gene="yceF"
                     /locus_tag="ACHKNP_RS03175"
                     /old_locus_tag="pgaptmp_000635"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HGU4702862.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(39583..40542)
                     /gene="rluC"
                     /locus_tag="ACHKNP_RS03180"
                     /old_locus_tag="pgaptmp_000636"
     CDS             complement(39583..40542)
                     /gene="rluC"
                     /locus_tag="ACHKNP_RS03180"
                     /old_locus_tag="pgaptmp_000636"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HGU4702863.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            41115..44300
                     /gene="rne"
                     /locus_tag="ACHKNP_RS03185"
                     /old_locus_tag="pgaptmp_000637"
     CDS             41115..44300
                     /gene="rne"
                     /locus_tag="ACHKNP_RS03185"
                     /old_locus_tag="pgaptmp_000637"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HGU4702864.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(44496..45449)
                     /gene="flgL"
                     /locus_tag="ACHKNP_RS03190"
                     /old_locus_tag="pgaptmp_000638"
     CDS             complement(44496..45449)
                     /gene="flgL"
                     /locus_tag="ACHKNP_RS03190"
                     /old_locus_tag="pgaptmp_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HGU4702865.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQATTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(45461..47104)
                     /gene="flgK"
                     /locus_tag="ACHKNP_RS03195"
                     /old_locus_tag="pgaptmp_000639"
     CDS             complement(45461..47104)
                     /gene="flgK"
                     /locus_tag="ACHKNP_RS03195"
                     /old_locus_tag="pgaptmp_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HGU4702866.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(47170..48111)
                     /gene="flgJ"
                     /locus_tag="ACHKNP_RS03200"
                     /old_locus_tag="pgaptmp_000640"
     CDS             complement(47170..48111)
                     /gene="flgJ"
                     /locus_tag="ACHKNP_RS03200"
                     /old_locus_tag="pgaptmp_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HGU4702867.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(48111..49208)
                     /gene="flgI"
                     /locus_tag="ACHKNP_RS03205"
                     /old_locus_tag="pgaptmp_000641"
     CDS             complement(48111..49208)
                     /gene="flgI"
                     /locus_tag="ACHKNP_RS03205"
                     /old_locus_tag="pgaptmp_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HGU4702868.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(49220..49918)
                     /gene="flgH"
                     /locus_tag="ACHKNP_RS03210"
                     /old_locus_tag="pgaptmp_000642"
     CDS             complement(49220..49918)
                     /gene="flgH"
                     /locus_tag="ACHKNP_RS03210"
                     /old_locus_tag="pgaptmp_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HGU4702869.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(49971..50753)
                     /gene="flgG"
                     /locus_tag="ACHKNP_RS03215"
                     /old_locus_tag="pgaptmp_000643"
     CDS             complement(49971..50753)
                     /gene="flgG"
                     /locus_tag="ACHKNP_RS03215"
                     /old_locus_tag="pgaptmp_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGU4702870.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(50891..51646)
                     /gene="flgF"
                     /locus_tag="ACHKNP_RS03220"
                     /old_locus_tag="pgaptmp_000644"
     CDS             complement(50891..51646)
                     /gene="flgF"
                     /locus_tag="ACHKNP_RS03220"
                     /old_locus_tag="pgaptmp_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HGU4702871.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(51666..52871)
                     /gene="flgE"
                     /locus_tag="ACHKNP_RS03225"
                     /old_locus_tag="pgaptmp_000645"
     CDS             complement(51666..52871)
                     /gene="flgE"
                     /locus_tag="ACHKNP_RS03225"
                     /old_locus_tag="pgaptmp_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HGU4702872.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(52896..53591)
                     /gene="flgD"
                     /locus_tag="ACHKNP_RS03230"
                     /old_locus_tag="pgaptmp_000646"
     CDS             complement(52896..53591)
                     /gene="flgD"
                     /locus_tag="ACHKNP_RS03230"
                     /old_locus_tag="pgaptmp_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HGU4702873.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(53603..54007)
                     /gene="flgC"
                     /locus_tag="ACHKNP_RS03235"
                     /old_locus_tag="pgaptmp_000647"
     CDS             complement(53603..54007)
                     /gene="flgC"
                     /locus_tag="ACHKNP_RS03235"
                     /old_locus_tag="pgaptmp_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HGU4702874.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(54011..54427)
                     /gene="flgB"
                     /locus_tag="ACHKNP_RS03240"
                     /old_locus_tag="pgaptmp_000648"
     CDS             complement(54011..54427)
                     /gene="flgB"
                     /locus_tag="ACHKNP_RS03240"
                     /old_locus_tag="pgaptmp_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HGU4702875.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            54582..55241
                     /gene="flgA"
                     /locus_tag="ACHKNP_RS03245"
                     /old_locus_tag="pgaptmp_000649"
     CDS             54582..55241
                     /gene="flgA"
                     /locus_tag="ACHKNP_RS03245"
                     /old_locus_tag="pgaptmp_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HGU4702876.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            55317..55610
                     /gene="flgM"
                     /locus_tag="ACHKNP_RS03250"
                     /old_locus_tag="pgaptmp_000650"
     CDS             55317..55610
                     /gene="flgM"
                     /locus_tag="ACHKNP_RS03250"
                     /old_locus_tag="pgaptmp_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGU4702877.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            55615..56031
                     /gene="flgN"
                     /locus_tag="ACHKNP_RS03255"
                     /old_locus_tag="pgaptmp_000651"
     CDS             55615..56031
                     /gene="flgN"
                     /locus_tag="ACHKNP_RS03255"
                     /old_locus_tag="pgaptmp_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HGU4702878.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(56071..57606)
                     /gene="murJ"
                     /locus_tag="ACHKNP_RS03260"
                     /old_locus_tag="pgaptmp_000652"
     CDS             complement(56071..57606)
                     /gene="murJ"
                     /locus_tag="ACHKNP_RS03260"
                     /old_locus_tag="pgaptmp_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGU4702879.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGTMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVM
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(57716..58639)
                     /gene="yceM"
                     /locus_tag="ACHKNP_RS03265"
                     /old_locus_tag="pgaptmp_000653"
     CDS             complement(57716..58639)
                     /gene="yceM"
                     /locus_tag="ACHKNP_RS03265"
                     /old_locus_tag="pgaptmp_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4702880.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTVLWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(58641..59288)
                     /gene="yceH"
                     /locus_tag="ACHKNP_RS03270"
                     /old_locus_tag="pgaptmp_000654"
     CDS             complement(58641..59288)
                     /gene="yceH"
                     /locus_tag="ACHKNP_RS03270"
                     /old_locus_tag="pgaptmp_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HGU4702881.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(59299..59883)
                     /gene="rimJ"
                     /locus_tag="ACHKNP_RS03275"
                     /old_locus_tag="pgaptmp_000655"
     CDS             complement(59299..59883)
                     /gene="rimJ"
                     /locus_tag="ACHKNP_RS03275"
                     /old_locus_tag="pgaptmp_000655"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HGU4702882.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPYNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            60119..61327
                     /gene="mdtH"
                     /locus_tag="ACHKNP_RS03280"
                     /old_locus_tag="pgaptmp_000656"
     CDS             60119..61327
                     /gene="mdtH"
                     /locus_tag="ACHKNP_RS03280"
                     /old_locus_tag="pgaptmp_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HGU4702883.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            61391..62038
                     /gene="grxB"
                     /locus_tag="ACHKNP_RS03285"
                     /old_locus_tag="pgaptmp_000657"
     CDS             61391..62038
                     /gene="grxB"
                     /locus_tag="ACHKNP_RS03285"
                     /old_locus_tag="pgaptmp_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HGU4702884.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPPRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            62172..62732
                     /gene="yceB"
                     /locus_tag="ACHKNP_RS03290"
                     /old_locus_tag="pgaptmp_000658"
     CDS             62172..62732
                     /gene="yceB"
                     /locus_tag="ACHKNP_RS03290"
                     /old_locus_tag="pgaptmp_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4702885.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            62838..63884
                     /gene="pyrC"
                     /locus_tag="ACHKNP_RS03295"
                     /old_locus_tag="pgaptmp_000659"
     CDS             62838..63884
                     /gene="pyrC"
                     /locus_tag="ACHKNP_RS03295"
                     /old_locus_tag="pgaptmp_000659"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGU4702886.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            63958..64203
                     /gene="dinI"
                     /locus_tag="ACHKNP_RS03300"
                     /old_locus_tag="pgaptmp_000660"
     CDS             63958..64203
                     /gene="dinI"
                     /locus_tag="ACHKNP_RS03300"
                     /old_locus_tag="pgaptmp_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HGU4702887.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            64493..64747
                     /gene="bssS"
                     /locus_tag="ACHKNP_RS03305"
                     /old_locus_tag="pgaptmp_000661"
     CDS             64493..64747
                     /gene="bssS"
                     /locus_tag="ACHKNP_RS03305"
                     /old_locus_tag="pgaptmp_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HGU4702888.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSELEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            64862..65980
                     /gene="solA"
                     /locus_tag="ACHKNP_RS03310"
                     /old_locus_tag="pgaptmp_000662"
     CDS             64862..65980
                     /gene="solA"
                     /locus_tag="ACHKNP_RS03310"
                     /old_locus_tag="pgaptmp_000662"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HGU4702889.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTVIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            66028..66141
                     /locus_tag="ACHKNP_RS03315"
                     /old_locus_tag="pgaptmp_000663"
     CDS             66028..66141
                     /locus_tag="ACHKNP_RS03315"
                     /old_locus_tag="pgaptmp_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HGU4702890.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT        16257 a        17625 c        16471 g        15894 t
ORIGIN      
        1 tggggaaatg gagccaactt cgacaacacg attttgcgcc gttcatacga acggcagggg
       61 atcccctgcc cgtggcgtta ctacaacgat cgcgatgtac gcacaatcgt tgagctgggg
      121 aaagccatag acttcgatgc cagaacggct attccattcg aaggtgagcg ccataatgca
      181 cttgatgacg ctcgtcacca ggcaaaatac gtttcagcta tctggcaaaa actgatcccg
      241 aatcaggctg atttttaatg ttcaaccgtc gccagttgtc gttgatattc tgcaactggc
      301 gcgttccgga gtgatagcca tgagcgaaca gtacctgata acgctcgacg agtggaaacc
      361 aaaacggttc agtctcccaa taacaaacac taccctggtg aaatacggaa aactaggata
      421 catcgttcca agaccacaaa aaattcgtgg gcgttggctg atagatcgcc gagcagtatt
      481 tgttgggcct ggtgaaacgg gaattgcgcc ggaaattcat actggcgatg atgatgcact
      541 gaaggagatt ttaactcatg tcaccgaggc cacgaaaaaa cagcactgac gtagccggtc
      601 tttacgaaaa gtttgatcgc agaactggca gagtttacta ccagtataaa aatcctgtga
      661 ctggaaaatt tcacggactc ggaacagaca aaggtaaggc agaaaaaatc gcttccacag
      721 ccaatcagcg aatagctgca gcagaagctg aatatttcat gcgcaaaatt gatgaaagtc
      781 cgtcagcaac aaaacgtcgg ggtatcagat taaaggcatg ggttgatcga tatctgaaaa
      841 tacaggacac gcgactgaaa aatggagata ttgcagctac aactcacaaa gaaaaaaccc
      901 gaatggctgc atacctggtt tcccgtctgg gaaaccaccc attgaaagaa ctggaagtaa
      961 gagactttgc attaatactg gatgagtggc tggataaaga catggtcagc acagcgagag
     1021 taaatcgtgg attatgggtt gatatttata aagaagcaca gcatgcaggg gaagttcctc
     1081 ctggatggaa tcctccggag gctacccgta aaccgatccc taaagtaacc agagccaggc
     1141 tcaccatgga agactggcaa aaaatttaca atgcaacgcc tgaaaaacac tttatccgta
     1201 acgcaatgct tcttgcgatt gttactggtc agcgccgtga tgacatttgc cacatgcgtt
     1261 tttcagatgt gtggaacgaa cacttgcata tcacccaggg aaaaaccgga atgcgtctgg
     1321 cgttaccgct tacactacgc tgtgatgcca ttgggataac gttaaaagaa gttattgatg
     1381 ggtgccgaga cagaatatta agtccatatc taatccatag tcggcaccag aaacaaccga
     1441 agccgatgag taaagacaac ctgagcgact actttgccaa agcacgggat ctggctggga
     1501 taattccacc agcaggaaaa actccgccaa catttcatga acaacgctct ctatcagaac
     1561 ggctgtaccg tgcacagggt attgatacaa aaacattact aggacataaa gtccaggcaa
     1621 ccaccgatcg ctataacgat actcgaggtc aggaatgggt taagttggtt atttgataaa
     1681 caccatatgg aatatccgtc accatctcag gaaaaaagtg ttgtaaaatg cgtggcctag
     1741 ttttggggaa acgttttgga gagattttgg agaaaggaaa aaattgaata aattcaaaat
     1801 cctgaaagtg tcccgcctta tgaataaaaa ggtggaactc gaatgatcgg tcgactgctt
     1861 cgcggcggtt ttatgaccgc tatctacgcg tacctgtata tcccaatcat tattttgatt
     1921 gtgaactcct ttaacagctc gcgctttggc atcaactggc agggttttac caccaaatgg
     1981 tatagcctgc tgatgaacaa cgacagcctg ttacaggcag cgcagcattc actaacaatg
     2041 gcggtgtttt cggcgacatt tgctacgctt atcggttcac tgacggcagt tgcgctgtac
     2101 cgttatcgct ttcgtggtaa gccgttcgtt agcggaatgc tgtttgtggt gatgatgtca
     2161 ccagatatcg tgatggcgat ttctctgctg gtgctgttta tgctgctggg tattcagctt
     2221 ggcttctggt cgctgctgtt ctcgcatatc accttctgcc tgccgtttgt ggtggtgacg
     2281 gtgtattcgc gcctgaaagg ttttgacgtg cggatgctgg aagcggcgaa agatctcggt
     2341 gccagtgaat ttaccattct gcgaaaaatc attctgccac tggcaatgcc tgcggtggcg
     2401 gcgggctggg tgttaagctt taccctgtcg atggacgatg tggtagtttc ttcgttcgtc
     2461 accggaccaa gttatgaaat tctgccgtta aaaatttatt cgatggtcaa agtcggcgta
     2521 tcgccggaag ttaacgcgct ggcaaccata ttactggtgc tgtcgctggt gatggttatt
     2581 gccagccagc ttattgctcg tgataaaacg aaaggtaaca caggggacgt taaatgaaaa
     2641 aatggtcacg ccacctgctc gcggcgggtg ctctggcact gggcatgagc gccgctcacg
     2701 ccgatgacaa caacacgctg tatttctaca actggaccga gtacgtgccg ccaggactgc
     2761 ttgaacagtt caccaaagaa accggtatta aggttatcta ttcgacttac gagtcgaacg
     2821 aaaccatgta cgccaagctg aaaacgtaca aagacggtgc ctatgacctg gtggttcctt
     2881 caacctatta cgtcgataaa atgcgtaaag aagggatgat ccagaagatc gacaagtcga
     2941 agttaacgaa cttcagcaat ctcgatccag acatgctcaa caagccgttt gacccgaata
     3001 acgattactc cattccgtat atctggggtg cgacggcgat tggtgttaac ggtgatgcgg
     3061 tggatccgaa atctgtcacc agctgggccg atctgtggaa gcctgagtac aaaggcagcc
     3121 tgctgttgac cgacgatgcc cgtgaagtgt tccagatggc gctgcgtaag ctgggctact
     3181 ccggtaacac caccgatccg aaagagattg aagctgcata taacgagctg aaaaaactga
     3241 tgccaaacgt cgcagcgttt aactccgata acccagctaa cccgtacatg gaaggcgaag
     3301 ttaacctcgg catgatctgg aacggttctg ctttcgttgc acggcaggcg ggtacgccaa
     3361 ttgacgtggt gtggccgaaa gaaggcggca ttttctggat ggacagcctg gcgatcccgg
     3421 caaatgccaa aaacaaagaa ggcgcgctga aattgatcaa cttcctgctg cgcccggatg
     3481 tggcaaaaca ggttgctgaa actatcggtt atccaacgcc aaaccttgcg gcgcgtaagc
     3541 tgttaagccc ggaagtggcg aacgataaaa cgctctaccc ggatgctgaa accattaaaa
     3601 atggtgaatg gcagaatgac gttggcgcag ccagcagcat ttatgaagag tattatcaga
     3661 agctgaaagc aggacgttaa ttcagcaagc gggcggtggc attaccgccc gttttttatt
     3721 tagcaaggat gtggctaatg agtcaggaca gtaaagtgtt atcccgcgct tttctcggta
     3781 tcggtctgat attgctcgtt atttctgcaa tcatctttta ttaccatttt acttttacca
     3841 aaagcgcggt tcatacagaa ggaatcattg ttgatgccgt ttggtacaac aatcactcaa
     3901 acgacgtcga tgataatggt tcctggtatc cagtggttgc ctttcgccct acgccagatt
     3961 acacgctaat tttcaattcg aatatcggta gtgattttta tgaagatagc gaaggtgaca
     4021 gagtcaatgt ctattatccg ccagggcatc cagaacaagc cgaaataaac aatccctggg
     4081 ttaatttttt taaatggggc tttgttggca tagcaggcat tattttcggt agtgtcggtc
     4141 tgataatcac gctcccctcc accaaaaaat ctcgcaggaa acggaagtcc tgcccatgaa
     4201 aaaattcatt atactgctca gtttactgat actcctgcct ctggtagcaa ccagtaaacc
     4261 attgattcca ataatgaaga cactttttac ggatgttacc ggtactgtac cggatgctga
     4321 agagatcgca cgtaaagccg agctttttcg ccaacaaacg ggaattgcac cgtttattgt
     4381 gattttaccc gacattaata atgaagccag tctcagacaa aatggtaaag cgatgctggc
     4441 gcatgcgtca tcttcattga gtaatgtaaa aggaagtgtt ctgttactat ttactacccg
     4501 cgaaccacgg ttaattatga tcaccaacgg ccaggttgaa agtggtctgg acgataaaca
     4561 tctcggcctt ctgatagaaa atcacacgct ggcttattta aatgcagatc tctggtatca
     4621 gggaatcaat aatgcattgg ctgttctaca agcacagata ttaaaacaac cgacgccgcc
     4681 actgacgtat tatccgcatc caggacaaca gcatgaaaat gctccccccg gcagcaccaa
     4741 cacattaggt tttatcgcct gggctgcgac gtttatctta ttttcctgga tcttttgcta
     4801 cacgactcgt tttatttatg cgttaaaatt tgcagtggcg atgactattg ccaatatggg
     4861 ttatcaggct ttatgtttgt atatcgataa tagctttgcg attaccagaa tatcgccact
     4921 ctgggcaggg ctaattggtg tctgtacgtt tattgccgca ttgttattga cgagtaaacg
     4981 ataaaagagg ccggaaaagt gcgcgtgtat tatgccggat ggggcgcgaa cgccttatcc
     5041 ggcccacaaa acccgcaaat tcaattaatt gcgtcccctt gtaggcctga taagcgtagc
     5101 gcatcaggca atgcttcctg ctttataatc ccttcagcaa cttttcaaca aactcaggca
     5161 ccacctggct tgccgggccg taatatttct cggcaaattc attaccgacc tgacttggtt
     5221 cgagattcag ttccacggtg tgcgcgccat gcagtttcgc ttcgtgaaca aacccagccg
     5281 ccggataaac atgcccggaa gtaccaatgg caatgaaaat atcagccatc gacaacgcca
     5341 tataaatttc atccatgccg agtggcattt cgccaaacca aacgacgtgc gggcgcaagg
     5401 gtgccgggaa ctggcaacaa tggcatttat cttctggcgt aacgtctccg gtccagtcga
     5461 gaacctgacc actttgcgaa caacgaactt tcagcaattc cccatgcata tgaatgacat
     5521 tggtattacc tgcgcgttca tgcaggttgt ctatattctg cgttaccagc aaaaagcgat
     5581 cgccgagggc gtcttgcagt ttagccagcg caagatgcgc ggcgttaggc tgaatttctg
     5641 gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg caccagttca ggatcgcgat
     5701 cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc ttcccacagg ccatctgcgg
     5761 cgcgaaaggt acgaatacct gattccgcag aaatccctgc ccctgtcagt acgagtactc
     5821 ttggtttttc cattgcttcc ggcaccactt tatctctgaa aaaaatacgc tgacgcaaac
     5881 gctcgcgcag gcggcgttta tttttacgaa aacgacttaa ccgatgaccc cgacgcgaca
     5941 gcatagcaac ctctgttgtt taatcggtta gatgtaggaa ggccgcacca cgcattcctc
     6001 ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt aagagatgac
     6061 gaggcagcct gtccgccagt tgcgttgtga ttgccgggaa attcgataag ccaccaccaa
     6121 tgacgaccag gtcagggtca acaatggtca ggatatttcc cagacaaacc gctaataaat
     6181 ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc ttgatcataa agcgcaataa
     6241 tttcgggagc ctgcaacggt tgatgataat agtgttgata cagccacgca aaaccgcgac
     6301 cagacagata attttcaatg cagccatgct gaccacagcc gcagcggcgt aacgggaaat
     6361 ccaaccccat catggttaac gcatcaaccg gcagacgcat atggccaaac tcgccggtaa
     6421 tgtagctttt accggtaatc ggtttgccgt tgaaaatcag cccgccgcca acgccggtgc
     6481 cgagaatcaa ccccatcacc agtggatatt gagtaaattc atcatcccag gcttctgaaa
     6541 gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt gcgctcaggt
     6601 cggcacgcag cggtttaccg ctggcagcgg ggacattggc ggcatacagc gtaccatctt
     6661 ctgtttccgg cattcccgga ataccgatgc cgacagaacc tttacagcca aaacgttggt
     6721 cagcttcagc taccagctca cacactgcat ctaaaaatgc gtcatagctg tcacgcggtg
     6781 tcggcacccg cttttcccac tgcaactgcc gaccgctatc aaacacgcca agcgcaattt
     6841 ttgttccacc aatatcaaac ccgtaataca ttgccgctcc ttgttttaat gtactgcctt
     6901 tactggccgc taaggactcg cgcagggtca atattactgg cgcgccgcgc cggataccaa
     6961 cttgccaaaa gactcagcaa caatgctgtg accagtacgt agaagacgtc cagccagtgc
     7021 aattccgatg gcaggaaatc aataaaatag atatcgctgg agaggaactg atgaccgatc
     7081 aacttttcaa tccactcaat aatcggggta agttgcagtg aaaccaccac accgataatc
     7141 accccacaca ggctgccgaa cagccctgcc agcaatccat accagacaaa gatggcgcga
     7201 attaaaccat ctttcgcccc cagcgttctt aatactgcga tatcgccact cttgtctttc
     7261 accgccatca ctaaggtgga gacgatgttg aaacaggcca cgccaatcac cagtaccatc
     7321 gccagataca taatggcgcg gatcatctgg atatcgcgat acatatagcc gtaagtacca
     7381 atccaacttt taatataaac atagctgttg gtcacttcac ccgcatcgcg taccagctta
     7441 ttggcgttga aaacatccgt cattttaagg gcaatacctg acacgctgga acccatatca
     7501 agatattgtt gggcatccgc cagcgggatc atggcaaaac tgtgatcgag ttgaccactc
     7561 aactgcaaaa taccggcaac gtgcaaacgc acacgttttg gctgcatcag tttatgctcg
     7621 ggattcgagt tggggatcat aatcgacacc caatccccct gcttcacttt cagcgcatcc
     7681 gccacgcctt tgccgatgat aatttgctgt tcgcccgctt taaaattgcg ccacgcatcg
     7741 ccctggacaa acgagggtaa tgcgctcaga cgctgttcct gttgcgggtt aacgcccttc
     7801 acctggattg cgcgaagatt cgcgccactt tccaccagcc cggtgaaatt gatatacggc
     7861 gcggcagcgg caatacccgg cactttttgc acgtgatcca gtgcttcctg ccagttagtc
     7921 cacggctgat ccaccgcctc gatttcacca tgcggcacca ccgccagaat acggttgttc
     7981 agttcgcgtt caaagccgtt catcgcgctt aagccgacga tcaataccgc cacgccgagg
     8041 gcaatgccaa tggtagaaat aacggagatc agcgacacca tgccgccgcg ccgccgtccg
     8101 cgactaaaac gcaggccaat taataacgat aaaggcatcg ccattactcc gcccccatca
     8161 ggctcagttc cgccgtcaga cgaccatcac gcatttctaa ctggcggctc atacgtttcg
     8221 ccagttgcag gtcgtgagta accaccagga aggcggtgcc ctgcaagcga ttcaattccc
     8281 caagcaactg gaagatgctg tcggcgttac gcgcatcgag gttaccggta ggttcatccg
     8341 ccagtaccag gcgcgggtta ttcaccagcg cacgggcaat cgccacacgc tggcgttcgc
     8401 cgccagaaag ttcagatggg cggtgattcg cacgatgatc cagccccacc gcttttaaca
     8461 tctcaagtgc acggctgttg atttcagcgg gctttttctt gccaatcagc agcggcatag
     8521 ccacgttttc cagggcggta aaatccggca gcaggtggtg aaactgataa ataaagccca
     8581 gcttctggtt gcgcagctct gctttcgccg ccgaagacag tttgctcatc ggttgaccgt
     8641 taaagatcac atcgccggag gttggcgtat ccagcccgcc caacaagtgc agcaaggtgc
     8701 ttttaccgga accagagcta ccgacgatcg ccatcatttc accttcgccg acgctgaaac
     8761 tgacattgtg cagcacatcg gtttgcacac tgccttcctg atagcgtttg cacaggttgt
     8821 cgcattgcaa caggatctta ttcataacgt aaagcctcag cgggttgagt ggcggcagcg
     8881 cgccatgaag ggtaaagcgt agacagcagc gcgatagcca tcgccaccag cgcaataaca
     8941 atgacctgta aaggttcgat agcgaccggc agcgccgcgc catcaagcag gacgccgatt
     9001 atcggcatca gattatttaa ctggctggca agcagtgcgc caagcgccgc accgaggatc
     9061 gcaccgataa tcccggcgct ggccccttgc accataaaga ccatcatgat ttgtcgcgga
     9121 gttaagcctt gcgtttgcag gatcgcgact tcgccctgct tctccattac catcagccct
     9181 agtgaggtaa taatgttaaa cgccgcaacg gcgacaatca ggctcagcag caagcccatc
     9241 atattttttt ccatgcgtac ggcctggaac agctcgcctt tacggtcacg ccagtcctgc
     9301 catttgctgc cttcaggcag tttttgctga cttaaagagt caactttcag cggctcatcc
     9361 agccacaaac gccagccggt aatattgcct gccggataac gcatcagacg cgaggcatcc
     9421 tcaatattca ccagcatttc atagccatcg acttcactgt tggcggcgaa agtgccaatc
     9481 acattgaaca ggcgctggct tggaatacgc cccatcggcg tgaactggct ggcagatggt
     9541 accatcacgc ggatttgatc accgcgatta acgcctagct gtgaggcaag ttgttcgccg
     9601 aggatgacat tatatttccc cggctcgagg tcagtttgtt tcacattgac cagatacggt
     9661 gtaagtggat ctttttgcgc cgggtcgata ccgagcatca ccccgaccgc cacgctgcgc
     9721 gcgctttgca gtaccacatc accggtagta ataggtgcga cgcgattaac gccgtccagt
     9781 ttgactgccg tttccgggag ttgctgcggg ttaagagagc catgctcaga agagagaatt
     9841 gcctgtggca tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc gttcatcact
     9901 gacaatactg tgaccagcgc catcaccccg agggtaatgc cgatggtaga aagccaggag
     9961 acgaaacgac cgaagcgatc cgctgcacgc ccacgcatgt aacgcaggcc aataaataga
    10021 gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc aaagacacga gtatataagc
    10081 gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt cagggctaat ctgaaaagcc
    10141 cccgtaaatc aatcggctaa attgccatgt tttatcatca atcttgccgc ctgatcttaa
    10201 tctgaaaaat catccaatac agacagatcc ccattacacc tcgggtcata acccgcagga
    10261 tcaaccgcat gtaaattaaa aaagagcgtg ttgtcctgat gaaacaaaaa gagctatgga
    10321 ttaaccagat caaagggtta tgtatttgtc tggtggtgat ttatcactcg gtcattacct
    10381 tttatccgca tctgaccact tttcagcatc cgttatcgga agtcctgagc aaatgctgga
    10441 tctatttcaa tctttacctt gccccctttc gtatgccggt ttttttcttt atatccggct
    10501 atttgattcg ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat aaacggatct
    10561 ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca gtggctggcg ttaagtgcgc
    10621 tgaaccagtg gctggcacct gagcgcgatt taagcaatgc ctccaatgcc gcttatgccg
    10681 attccaccgg tgagttcctg cacgggatga tcaccgccag caccagcttg tggtatctgt
    10741 atgcgttaat tgtctatttc gtggtatgta aaatttttag ccgtctggcg ctgccactat
    10801 tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt tcccacgccg tggtggggaa
    10861 tgaacagtgt gatccgcaat ttgctttatt acagccttgg cgcatggttt ggcgcaacaa
    10921 taatgacctg tgttaaagag gtgccgttgc gccgccatct gctgatggct tctttgctta
    10981 ccgttctggc ggtcggtgcc tggttgttta ctatctcgct gctgttgtcg ctggtatcga
    11041 ttgtggtgat catgaagctg ttttatcaat acgagcaacg attcggtatg cgctccacca
    11101 gcctgctgaa tgtgattggc tccaacacca ttgctatcta caccacccat cgcattctgg
    11161 ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc agcacgctgg tcgccgcaag
    11221 tcgaactgac actcctgctg gtttatccct ttgtcagttt gttcatctgt actgttgcgg
    11281 gcttgctggt aagaaaactt tcacagcgtg cattcagcga tctgttgttc tccccgcctt
    11341 cactgcccgc tgccgtcagt tactctcgtt aaaccatcgc cccagccggg gcgattcaat
    11401 ttgctaaacc atgtcgatta cggataatga acagcattgc ttatcagaga tatgccccca
    11461 tatgttgagg catatcctaa cgagaatctg acaaccgtta tgcctgaaca atatcgttat
    11521 acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg agttaaccgg cgcagcctgt
    11581 gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc cggtggtact cattgcacca
    11641 gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc agttcaccga tcagatggtg
    11701 atgaatctgg cggactggga aactcttccc tacgacagtt tttcgcctca tcaggacatt
    11761 atctcctcgc gcctttccac cctttaccag ctaccgacga tgcagcgtgg cgtactgatt
    11821 gttccggtga atacgcttat gcagcgcgtt tgcccacaca gttttctcca cggtcatgcg
    11881 ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat tacgaaccca actggacagc
    11941 gccggttatc gccatgttga ccaggtgatg gagcacggcg aatacgccac gcgcggcgcg
    12001 ttgctggatc tcttcccgat ggggagtgag ctgccttatc gtcttgattt ctttgatgat
    12061 gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc gcacgctgga ggaagtagaa
    12121 gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata aagcggcaat tgaactgttc
    12181 cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc cggaacatat ttaccagcaa
    12241 gtgagtaaag gcacattacc tgccgggatc gagtactggc agccattgtt cttcagcgaa
    12301 ccactgccgc cgctgttcag ttatttccct gccaatacct tgctcgtgaa tactggcgat
    12361 ctggaaacca gtgccgaacg tttccaggct gacacgctgg cgcgttttga gaatcgcggc
    12421 gtcgatccga tgcgcccgct gttgccacca caatcgctct ggctgcgggt ggacgagctc
    12481 ttctcagagc tgaaaaactg gccccgggtg cagctaaaaa ctgaacattt accgacaaaa
    12541 gccgcgaatg ccaatttagg tttccagaaa ctgccagacc tggccgttca ggcacaacaa
    12601 aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt tcgacggtcc ggtggtgttc
    12661 tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac tgctcgcacg aattaaaatt
    12721 gctccgcaac gcattatgcg tcttgatgaa gccagcgacc gtgggcgtta tctgatgatt
    12781 ggcgctgccg aacatggttt tgtcgataag gtgcgtaatc tggcgctgat ctgcgaaagc
    12841 gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt ctcgccgcac catcaacccc
    12901 gatacactga tccgtaacct tgcggagctg catattggtc agccggtggt ccatctggag
    12961 cacggtgtcg ggcgctatgc cggaatgacc acgctcgaag cgggcggcat tactggcgag
    13021 tatttgatgc tcacctatgc caacgacgcc aaactgtatg ttccggtgtc gtcactgcat
    13081 ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc cgctgcataa acttggcggc
    13141 gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag tgcgtgatgt ggcggcggaa
    13201 ttgctggata tctacgcgca acgcgccgcc aaagagggct tcgcgtttaa acacgatcgt
    13261 gagcagtatc agttgttctg cgacagcttc ccgtttgaaa ccacgccgga tcaggcgcag
    13321 gccattaatg cggtacttag cgacatgtgt cagccgctgg caatggatcg tctggtgtgc
    13381 ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg ccgctttcct ggcagtagat
    13441 aaccacaagc aggtggcggt gttggtgcct accacccttc tcgcgcagca gcattacgac
    13501 aacttccgcg accgtttcgc caactggccg gtacgtatcg aaatgatctc ccgtttccgc
    13561 agcgccaaag agcagacgca aatccttgcg gaagtggcgg aagggaaaat cgatattctg
    13621 atcggtacgc acaaactgct gcaaagtgac gtcaagttta aagatttagg cctgctgatt
    13681 gtcgatgaag aacaccgctt cggggtgcgt cataaagagc gtattaaagc gatgcgcgcg
    13741 aacgtggata ttctgacgct tactgcaacg ccgatcccac gtacgctgaa tatggcaatg
    13801 agcggaatgc gtgacctgtc gattatcgcc acgccgcccg cccgtcgtct ggcagttaaa
    13861 acctttgtcc gtgagtatga cagcctggtg gtccgggagg cgatcctgcg tgaaattttg
    13921 cgcggaggac aggtttatta tctctacaat gatgtggaaa acattcagaa agccgccgaa
    13981 cggctggcag aactggtgcc agaagcgcgg atcgccatcg gtcacgggca gatgcgcgag
    14041 cgcgaactgg aacgggtgat gaatgatttc catcatcaac gtttcaacgt gctggtttgt
    14101 acaaccatta tcgaaaccgg gatcgacatc ccgacagcca acaccattat cattgaacgc
    14161 gcggatcact tcggtctggc gcagctgcac cagttacgcg gtcgcgtcgg acgttcacat
    14221 catcaggcat atgcatggtt gctgacgccg catccaaaag cgatgactac cgatgcacaa
    14281 aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg caggttttgc gctggcaacg
    14341 cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg aagaacaaag cggctcaatg
    14401 gaaaccatcg gtttctcgct gtatatggag ttgctggaaa acgccgtcga tgcactgaaa
    14461 gccggacgcg agccgtcgct ggaagatctc accagccagc aaacagaagt cgagctgcgg
    14521 atgccgtcgc tattgccaga tgatttcatt cctgacgtga atacgcgttt gtcgttctat
    14581 aaacgtattg ccagcgccaa aacggaaaac gaactggaag agatcaaagt cgagcttatc
    14641 gatcgcttcg gcctgctgcc ggatccggcg cgtaccctgc tggatgttgc ccgtctgcgc
    14701 cagcaagcgc agaaactggg gatcaggaag ctggaaggta atgagaaagg cggggtgatc
    14761 gaatttgccg agaagaatca cgttaatccg gcctggttga ttggtttgct gcaaaaacag
    14821 ccgcagcatt accgccttga tggtccgacg cgcctgaaat ttattcagga tttgagtgag
    14881 cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg aactggaaga gaacgcgatc
    14941 gcttaatggt aaatcggggg cgtttctgcg cccccatctg caacatttac aaattctttg
    15001 cacttccctg cactatccga cactgtcacc atccataatt caggcttatc tgtttattac
    15061 aataacctta tatttattat ggatttttgg tgatgatcaa aacgcgtttt tctcgctggc
    15121 taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa gccaacacct
    15181 ggccgctgcc gccagcgggc agtcgtctgg ttggcgaaaa caaatttcat gtggtggaaa
    15241 atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt ctcgctctgt
    15301 tacaggctaa ccctggcgtt gatccttacg taccgcgcgc gggtagcgtg ttaacgatcc
    15361 cgttgcaaac cctacttcca gatgcgccgc gcgaaggcat tgtgatcaat attgcggagc
    15421 tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca ataggtattg
    15481 gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca gacaaacgtg
    15541 caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca cagggaattg
    15601 agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat gcgattcgtc
    15661 tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc ggcattggca
    15721 tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca ctctttagcc
    15781 aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc tctgccgaac
    15841 caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt gatgacgatc
    15901 cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat gcagcacaaa
    15961 ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg gtggatgtcc
    16021 gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat ccccgcgaca
    16081 cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta atgcttattt
    16141 ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa tacggaaaga
    16201 ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc ccagatttgt
    16261 ccccgcgtta gcactgattg taccgacttt ttgttggcct tctggcgttg actgaacttc
    16321 gacagccgca aagctggcaa atgacatgga gcttaaaatc gccgcagcga tgagggtttt
    16381 tacgtttttc ataatagtgg ccttatgcag atgaatgacg tcgcaagcaa ttacttaacg
    16441 atcgataact aaatgataga tgtgatctgg atcacataca agatattttt tataacaatc
    16501 attaattaat ttaaaaatct ttaaatttca gttaaataga aagcaattat ttttatggga
    16561 tctggactgg tgaacggagg tgattatttt tataataacc tttcattcac agtcaccagg
    16621 tacaacggca tcatggcaac tgactcaaca caatgtgtaa aaaaaagccg tggccgccca
    16681 aaagtgttcg acagggatgc cgcgcttgat aaggccatga aattgttctg gcaacacggt
    16741 tatgaagcga cttctcttgc ggacctcgtc gaagcgaccg gagccaaagc gcccacgcta
    16801 tacgcggaat ttaccaacaa agaggggtta tttcgtgccg ttctcgaccg ctatatcgat
    16861 cgttttgccg ctaagcatga agcacagctg ttttgtgaag agaaaagcgt ggagtctgcg
    16921 ctggctgact attttgctgc catcgccaac tgctttacca gcaaagacac cccggcaggc
    16981 tgcttcatga tcaacaactg caccaccctc tccccagatt caggagatat cgccaatacg
    17041 ttgaaatcac gccatgcgat gcaagagcgc actttgcagc agtttttatg tcaacgacaa
    17101 gcgcgcgggg aaatcccgac ccactgtgac gtgacacatc tggcagaatt ccttaattgt
    17161 attattcagg ggatgtcgat cagcgcacgc gaaggtgcat cgctggaaaa actaatgcag
    17221 attgccggaa cgactttgcg tttatggccc gaactggtga aataagtcgc aaaaaaagcc
    17281 cctcagcctg agcgaatgag gggccaaatt gcagagtaca gccttgttat actttgttat
    17341 tcaaaatcag ttgcccatta ctatctagcg ggatctgcgt acccggatcg cggtccatgc
    17401 ggattttgcc ctgctgatcg ccaatcttat aggtcacatc ataaccgagc attttttctg
    17461 acttgtcata caccgtttta caacgctgtt gcgtagtcgt gtaagtatcg ctttcctgga
    17521 gagagccctg gatctggtta ccggcatatc caccacccag cgcccccaca acagtggcga
    17581 catctttacc gcgaccaccg ccaaactgat gccctatcac gccgccagca acagcgccga
    17641 gcaccgaccc ggtaatgcga ttttcatcct gcaccggtcg acgatgggtc actgtgacgt
    17701 tgcgacactc ctgacgcggt gttttaaccg tttccttgat tggggttgca gaaacaacct
    17761 gagcgtattg cgggccacgt tcaaacacgt tcagactggc cactgccgct acgcccagcg
    17821 cagctgcgac accaatcccg atacccgcca acattgattt attcacggga acacctcctt
    17881 ctttgcagtt atgccaatca tgcaagcgaa ggagaaaagc agctatcaga cagaagatga
    17941 aaagcgcggg aatggctgga aatacttaat gattcggagg agtctgaagg aatgcctgat
    18001 gcgacgctta acgcgtctta tcaggcctac cgctcgtacc atttgtccag ggcggatgag
    18061 gcgtttatgc cacatccgcc agtgtacgtc gattaatgca acttcaaacg cggacggata
    18121 acgcggttaa tactccccac cagcatcatt aatccggttt taaagtaacc atgcagcgca
    18181 atctgatgca ttcggtatag cgagatatat acaaagcgcg caattcgtcc ttcaatcatc
    18241 attgagccgc gcgtcaggtt acccatcagg ctaccaacgg tggagaagtt cgacagcgat
    18301 accagcgaac catgatcttt atactgataa tttttcagcg gcttaccatt catctgcgcc
    18361 agaatgttgt tcattgcgca agtcgccatc tggtgtgcag cctgagcacg cggcggaaca
    18421 aagccccctt ccggacgcgg gcatgacgcg cagtcgccaa tagcgtaaat gtctggatcg
    18481 cgggtggttt gcagcgtcgg ttccaccacc agctggttga tacggttagt ttcaagacca
    18541 ccgatatctt tcaggaagtc tggcgctttg atcccggctg cccacaccat cagatcagcc
    18601 tcaatatatt cgccatcttt agtgtgcagg ccgccttcat cagcactggt gaccatggtt
    18661 tgcgtcagca cgcgaacgcc aagtttcgtt agctcgttgt gggccgcagc agagatacgt
    18721 ggcggtaatg caggcaaaat acgttctccc gcttctacca gcgttacgtt cagggcttcg
    18781 ttggtcaggc ctttgtaacc gtagctgtgc agttgcttga ccgcgttgtg caattcagcg
    18841 gagagttcta cacccgtcgc gccgccgccg acaatcgcaa tgttcacttt gccatttgcg
    18901 cccaggttgg cggagtattt caggaagaga ttcagcatct cctggtgaaa gcgacgcgcc
    18961 tggtgcgggt tatcgaggaa aatgcagttc tctttgacac ctggcgtatt gaaatcgtta
    19021 gaggtgctac ccagcgccat taccagggtg tcataggcga ttttacgttc cggaaccagc
    19081 agttcacctt tctcatcgcg cagttctgca atagtgattg ttttcgcttc acgatcaata
    19141 tcaatgacgg aacccagctg gaactggaaa ccatgattgc gcgcatgggc cagatagctc
    19201 aacgcatcga cgccttcatc aagcgagcca gtcgccactt cgtgcagcag cggtttccac
    19261 agatggctgt ggttacgatc gaccagcgta attttggctt ttttcttgcg tcccagctta
    19321 tgccccagct gtgttgccat ttccagccca ccagcaccgc cgccgacaat cacaatcttt
    19381 ttcaatggcg tagtcaacgt gaccccctta aatttattaa ccaattgtta attaatggtt
    19441 attaacatag ctcttaatta acaacaggtt acagcaatga aaatgttctt cgctattgag
    19501 aataacatga atggtgcatt ggtcatacca aaattgatgt taatcaagtt ttgttgctga
    19561 aaagataggc gactttaccg ggtgagatca aaaaacagag tttattgttt attatttgag
    19621 caagtccgga agggcgatat agggtgaagc gtatatcgag ggcgtttggt cgtcctgata
    19681 agaggcgcat caggcattga tgctcgaatg tcggatgcgg cgtgaacgcc ttatccgacc
    19741 cacagaatcc ggagcgcatc aggtaccggc aaggttacct gatgcgacga gggcatttac
    19801 ccgagggttt tgaacgcttt aatgcgctgt aaatgcgggg agatattctt gaatttgtgc
    19861 gtctgctctt cgtcccagac aatctcgtaa taatgatgca gctcttcaga tgtccgctgg
    19921 ctgttaagcg cttcatcatt acgcgacaaa atcaccaggc aacgatcgcg attcttctca
    19981 cggaagttgg tcacacactt agtggcaata tcggcatact cttccgggcg atcaatcttg
    20041 ccttccatgt tctcataagg gaacaaatta gggttgaaga tcacctggcg gatatcgcaa
    20101 agaaaaccaa tccgttccgc ccagtatccg cccagaccaa cgccgcaaat cagcggacgc
    20161 tcgtcgacgt tcagttgcaa cattttgtcc acttctttga gcagatgctg catatcatgt
    20221 ttcggatgcc gcgtactgta gcttatcaag cgtacatccg ggtcaataaa ctgcaattgt
    20281 aagactttct cgtggttacc cggactgtta gagtcaaaac cgtgtaaata gataatcatc
    20341 gcttcctcac ataagccagg gttagtgacc tgctttctct tcctgccagc gttcatgtaa
    20401 ctgattcaga cgggtgctga tcgctttcca gcgagccgag tccatcagtt cctgtcgcga
    20461 aaatgaacct ttatgataca aacgtgtaac acgttctgcc ttgatcggtg acagattatc
    20521 taacacgctg acggcccctt tacgattatt gcagaccagg atcatatcgc aacccgcatc
    20581 cagtgatgcc tgcccgcgtt cggcataact gcccataatc gcggcacctt ccatcgataa
    20641 atcgtcagag aaaatcacgc cgtcaaaacc cagttcctga cgcaaaacgg ttttcagcca
    20701 gtagggagaa ccgctcgccg gacgcggatc aacatcactg tagatcacat gcgcaggcat
    20761 aatggcgtcg agtttatttt cgcggattaa ggaactgaag accgacatat ctttagcgcg
    20821 aatctccgct tgtggacgtg gatcgcacgg tgtttctttg tgtgagtctg ccgttactgc
    20881 accgtgtcct gggaagtgtt tcccggtcgt tttcattccg gcttcatgca taccatcaat
    20941 aaaccggctg gcaattgcca gggctttttg tggatcggca tgataagaac gctcgccaat
    21001 cgccgcgctg atatgcccga catccagcac tggcgcaaag ctgatatcaa tatccatagc
    21061 gatcatttcg ctggccatca gccaacccgc ctcttgcgcc agtttgccgc cctcttccat
    21121 tcctgacagc gcagcgaatg attgcgccgc tggcaagcgg gtaaaacctt cacgaaaacg
    21181 ctgcacgcgt ccaccttcct gatctaccgc caccaccaga tgattgcgcg aagctgcgcg
    21241 gatctggcgc accagttcac gcaactgggc aggatcatga tagttacgcg taaagagaat
    21301 cagccctccc accagcggat gcgcaagtat ttcacgctct tccgcgtcca gttcgtaacc
    21361 ttcgacatcc aacattactg gacccacact gctctcctta ttgttttatt aatagctgcc
    21421 gccaggtgtc atcggccagc ctgataaatt gttgatcgcc ggtttgtcgc cagcggtact
    21481 caaaccaccc tgctttgagc atcagcagcc agggaaacca tcgcctgacc tgacgccata
    21541 attgcgccgg ataaatcttc gcgcgagtgg catagtcatt gaccaattgc cggtgctgtt
    21601 cagtattttc cacccacact gccgccagtt ccagcgcgat atcaccatct ccggcatact
    21661 cccagtcgat gagttttaac cctgacgcgc tatgcactaa atttccggcg tggacatcca
    21721 tatgcaatgg acttaagcgt aaaggccgtg gttcccgcgc tttgcgcaga cgttttaaca
    21781 ttcgcagcca acccactgtc cgccgcgccg gatcgctttg ctgccagtac agttccagta
    21841 acggcaacag cgttattcgc cagccaaaac gtggttgttg atgtagataa tacagcaagc
    21901 ctgccagttc gttggtatcc ggcaaatacg tttttacctc gccgggcaga tagtcgacta
    21961 ccatccagtc acggagatat aaatgcggct tcggtgcaat gcttgcgggt agttgtgata
    22021 aagcccgata ctggcgcaag aacgcggact gcggcgcatc aggatcgtgc ggctgacgca
    22081 caacaaaacg ctggccctga tgttcaatga gaaaactccc gccactaagc ccactattaa
    22141 acgtcgtgac gggatgaaac tgcgggaaaa agcgcgacag caattcgtcg cgcatgatgg
    22201 gattattgct gcgaaacggc acctttacct gaccagataa tttcgcccgt ctgcaccagc
    22261 atcagctgca tttgtagggt cggagcgtta acgttgccag aggcgctgga gtacagcacg
    22321 taatgagcgc cgacattgcg ggcaatgcct atggctttac tacgggtacc taaactgtcc
    22381 tgcggcgaca aacctaactg ttgcttagcc atcgacagct gctgggcgga aaccagggta
    22441 aatttcccgt tattagccag cgcatttcgc agcgtttcgg tcgcttctgc ggcattcagc
    22501 gaaccgttag tacggttgtt aacgctatca accagcagga cgctacccgc agtcaccccg
    22561 tcagccccaa gcatcttact gaccatcggc tgcattgcgc cattccagtc ataatggcga
    22621 atatgcggcg caggcggtgc cgtttgatct tcgtgctcaa ttgggcctgg ctgctgcggg
    22681 atcgtcggca ccgagggcac tgtggggaca ggctgttgtg gctcggctgg ttgttccggc
    22741 gctggtttca cttcttctac cggtgcaggt tcacgttgcc ccacacaccc ggcgagaaac
    22801 atcgccagcg cggtaatcaa ggcgtagcga ctcatttttg tcatcaagat tcacccctta
    22861 caaatataga taaagtctga ctttgtgcgc ccccagaaaa ttggcgctgc cgtacagcgt
    22921 taccgccgaa tgtgcgggaa tggtaacgct gcgtggcctt tccagaggat gcatctccag
    22981 ccctctggcg tcataccagt aaaaacgata atgaacggta acgggttctt gcctttcgtt
    23041 ataaagcgtt gaggatgctg aaggttgaat atcagacgtc gaaaggacgg gcttttctgc
    23101 actgatgccc gcagccaata aagatgactc catcaccagc gattgctcat cattcaccgg
    23161 aatttccgga tgtgaacgac agcccaccag cagtaacaac gccagagaca ccagcccaaa
    23221 gcatcctttt ctcatcgtta cagaccttta tgtgccagca ttggtcccag cggacggcca
    23281 cccaacaagt gcatatggat gtggtaaacc tcttgtccgc catggcggtt ggtattcatg
    23341 atcagacgat agccatcttc ggcaatacct tcttgctcag caatttttgc cgctacggtg
    23401 atcatgcgtc ccagcgcctg ctcatgctca gctgacacgt cgttcacagt cggaatgagg
    23461 atattcggaa tgatcagaat atgcgttggc gcttgcggcg aaatatcgcg aaacgccgtt
    23521 accagatcat cctggtagac gatatcggag gggatctcac gacgaataat tttgctgaat
    23581 atagtttctt ctgccacgac attttccttt ttcaaatagc catgcgttgc gccatgctac
    23641 gccgcgccac actgcgagta tagagtatgg tcgagttaaa cctgctcttt caaccttaac
    23701 tcacgatttc ttaagcaaaa aatcgttttg ctgccgtata tatcgccatt attcccattt
    23761 ctgcctgcga atgcgctgaa aagacattct ctgtttggtt tacacgtcaa atatggatgc
    23821 gtatatttct catttgcatt tacatacaaa attattcgca cactaaatat aaccgctcat
    23881 caccctcctc cggcatgagc ctgacgacaa cataaaccaa gagatttcaa atgctttcaa
    23941 cacaatttaa cagggataat caatatcaag ccagcaccaa accatcacta cttgccggtt
    24001 gcatagcatt ggcactatta ccttctgccg cttttgccgc tccagccact gaagaaacgg
    24061 tgattgttga gggttcagcc acagctccag atgatggcga aaatgattac agcgtaacgt
    24121 ctacctctgc gggtaccaaa atgcagatga ctcaacgtga tattcctcag tcggtcacta
    24181 ttgttagcca gcagcggatg gaagatcagc agttacaaac gctgggcgaa gtgatggaaa
    24241 acacgctggg gatcagcaaa agtcaggcgg attccgatcg tgctctttat tattcccgcg
    24301 gattccagat cgataactat atggttgatg gtatccccac ctattttgaa tcgcgctgga
    24361 atctgggcga cgcactttct gatatggcac tctttgaacg cgtagaagta gtgcgtggcg
    24421 cgacaggact catgaccggg acgggtaatc catctgcggc aattaatatg gttcgaaaac
    24481 acgcgaccag tcgtgaattt aaaggcgatg tctcggcgga atacggtagc tggaacaaag
    24541 aacggtatgt ggcggattta caaagcccac tcaccgaaga cggtaaaatc cgcgcgcgaa
    24601 ttgtcggcgg ctaccagaat aacgactcat ggctggaccg ctacaacagt gaaaagacct
    24661 tcttctccgg cattgtcgat gctgatttag gcgatcttac gacgctttca gccggttacg
    24721 aatatcagca cattgatgtt aatagcccaa cctggggcgg tttaccgcgc tggaatactg
    24781 atggcagcag caacagttac gatcgcgcac gcagtaccgc acctgactgg gcgtacaacg
    24841 ataaagagat caacaaggtc tttatgaccc tgaagcagcg gtttgctgat acctggcaag
    24901 cgacgctgaa tgccacccac tctgaagtcg aatttgacag caaaatgatg tatgtcgatg
    24961 cctatgtaaa caaagcggat ggtatgctga ttgggccata cagtaattat ggacctggct
    25021 ttgattatgt cggcggcacc ggttggaaca gtggcaaacg taaagttgat gcgctggatt
    25081 tgttcgctga cggtagttat gaattgtttg gtcgtcagca caatctaatg tttggtggca
    25141 gttacagcaa acaaaacaat cgttacttca gttcatgggc caacatcttc ccggatgaaa
    25201 ttggcagttt ctacaacttt aatggcaatt tcccacaaac cgactggtca ccacagagcc
    25261 tggcgcagga cgataccaca catattaaat cgttatatgc tgccactcgt gtcacccttg
    25321 ccgatccgct gcatctgatc ctcggcgcac gttataccaa ctggcgggtt gatacgctga
    25381 cttacagcat ggagaaaaac cacaccacgc cttacgctgg tctggtgttt gacatcaatg
    25441 acaactggtc gacctacgcc agctatacct ctattttcca gccgcaaaat gatcgtgaca
    25501 gttcaggcaa atatctggct ccaatcaccg gtaacaacta cgagctgggt ctgaaatcgg
    25561 actggatgaa tagccgtctg accaccacgt tagccatctt ccgtattgag caggataatg
    25621 tcgctcagtc caccggtaca cctatccccg gcagcaacgg cgaaaccgcc tataaagcag
    25681 tggatgggac agtcagtaaa ggggtggaat ttgaactcaa cggcgcaatt accgacaact
    25741 ggcagctgac atttggcgca acgcgctata ttgcagagga taacgaagga aacgccgtta
    25801 atcctaatct gccacgcacc acggttaaaa tgttcaccag ctatcggttg cctgtcatgc
    25861 cagagttgac agtcggcggt ggtgttaact ggcaaaatcg cgtgtatacc gacaccgtga
    25921 caccgtatgg caccttccgc gccgagcaag gtagctacgc gctggtggat ctcttcaccc
    25981 gctaccaggt gacgaaaaac ttctcgttac aggggaacgt caataacctg ttcgacaaaa
    26041 cctacgatac caacgtggaa ggttctatcg tctacggcgc accgcgtaat ttcagcatta
    26101 ccggcacgta tcaattctga atagcacctg taaaaaaggc agccatctgg ctgccttagt
    26161 ctccccaacg tcttacagat tagtggttac ggatgtactc atccatctcg gttttcaggt
    26221 tgtcggattt agtaccgaaa atcgcctgaa caccagaacc agcaaccact acgcccgctg
    26281 cgcccagttt cttcaggcca gcctgatcca ctttagacac atcagcaacg ctgacgcgca
    26341 gacgggtaat acatgcgtcg aggttagtaa tgttttcttt accaccaaat gcagcaacca
    26401 gagccggtgc catttcgctg gtacctgtcg cttttgcatc ttcagtcgcg tcttcacgac
    26461 ccggcgtttt cagatccagt gctttaatca gcacgcggaa gatggtgtag taaacaatcg
    26521 cataaccgat accgacgatc gggaacagcc acagtttgct gctgttacca gacagaacga
    26581 tgaagtcgat cagaccgtgc gagaacgacg taccgtcacg catccccaga agaatacaga
    26641 ttgggaatgc caggcctgcc agaatcgcgt ggatgatgta caggatcggc gcaacgaaca
    26701 tgaaggagaa ctcgatcggc tcggtgatac cggtcaggaa cgaggtcagc gccgcggaga
    26761 tcataatacc gcccactttc gcgcggtttt ctggtttagc agagtgccag atagcaattg
    26821 cggcagctgg cagaccgtac attttgaaca ggaagccacc agacagttta cccgcagtcg
    26881 ggtcacccgc catataacgc ggaatgtcgc cgtggaaaac ctgacctgct gcgttggtgt
    26941 attcaccaat ctgcatctgg aaaggtacgt tccagatgtg gtgcagacca aacggtacca
    27001 ggcaacgttc gatgaaaccg taaatgccaa acgcaactac cgggttctgg taagcagccc
    27061 actgagagaa ggtctggatt gcagaaccaa tcggcggcca aatgaaggac agcacaacgc
    27121 cagtaaagat ggcagccagg ccagaaatga tcggcacaaa gcgtttaccg gcaaagaagc
    27181 caagatactc aggcagctta atacggtaga aacggttaaa catgtacgct gcgatcgcac
    27241 cggagataat ccctccgagt acgccagtat ccgccaggtg tttagaggcg atttcttcag
    27301 caggtaaatg cagtaccagt ggcgcaacca cggccatggt tttaaccatg atgccatagg
    27361 caacaactgc ggccagcgcg gatacgccat cgttattggt aaagccgagg gcgacaccga
    27421 tcgcaaaaat cagtggcatg tttgcaaaga cggaaccgcc tgcttctgcc ataacatgcg
    27481 atacaacggc gggcagccag ctgaaattcg cggaaccgac gcccagcaga atacctgcga
    27541 taggcagtac ggataccggc agcatcagcg atttaccgac cttttgcagg ttagcaaatg
    27601 cattcttaaa cataattgag agtgctcctg agtatgggtg ctttttttac gttctcacgc
    27661 gtggcaaggg gggagagcct cgccgtgtac agggcatcta agcgcccttt atttattaca
    27721 cagagtaaaa taattcagtg ccaatttgtt tgacggctat cacgtttcaa cttcagatca
    27781 ttggagaagt tgcacatttt gacaaaacat atgtcacaat atttcaaacg tggttattca
    27841 ccgcccaatt ttcggcggtg aactttactc gttttagcca ttaattacga gctttaaaaa
    27901 aactcattca acggatggat tgaaggcggg aagcgtcgat gtgaaacaga cgggcgaagt
    27961 tatcggtggt tacctgcgcc agttcttcaa cggcaacacc tttcaacaca gccatgtatt
    28021 ctgcaacgtc acgaaccatc gcaggttgat tctcttttcc tcgatgcggt accggcgcaa
    28081 ggtaaggtga gtcagtttcc accagtaatc gatccagggg gacataacgc gcagcatcgc
    28141 gcagttgctc cgcattacgg aaggtcacaa tgccggaaaa ggagatgtaa aatccgagat
    28201 ccagtaattt acccgccgtt tctctgtcct ctgtaaaaca gtgtagtacg ccaccgcaat
    28261 ccgtcacttt ttcttcgcgc agaattgcca gcgtatcggc acgggcgtca cgggtatgga
    28321 cgataaccgg cttgttcagt tcacgaccaa tctggatatg atggatgaag gactcttgct
    28381 gacgtacttt agtttccggt gtgtaataat aatccagccc ggtttcaccc agcgctacaa
    28441 caccctcttc tgccgccaga cggcgtaaat cttctacatc gtagggatca ttctggttga
    28501 gtggatgcac gccacaagaa aatacgacgt tgtcacgttc gcctaccaga tcccgcatat
    28561 gtaaataacc cggtaatgtt gtggcgactg ccagacaaaa tttcacatcg cgtgcggcgg
    28621 ctttcgccag aacgtcatcc acgtccttat gcaaagattc ataatccaga ccatcgagat
    28681 ggcagtgtga gtcgactaaa aacatgatgt ctctcttaaa gatgaggaac cggtagcaca
    28741 acgcccggtt gcaggtaatg ctcaatacgc agtaaaagat cggtgatgag aagctcgcgg
    28801 ttgatgcctg taacagacat taactgttca cgaatgtggc aaacatcccc cagtatagcc
    28861 tgcaggcgcg agggagaaag atggtttgcc agttcggcga ccaggcccgg cacatcaaca
    28921 ttggtcacct gcgcagcacc atgatggcgt tttagcgcat ccatcagcaa cgttgccagc
    28981 cagtgtaaac gcgccggagc ttgttcatga ttaagggccg ctagcagcga ataccagtcg
    29041 cccgatggca cgctatatgc caacgcctga cacaatgttt cacgagcctg ccagttatct
    29101 ccctgaaaca acgccagtgc cgcgccaggc gaaccggcgc ttaagcgcaa tgcggcaagt
    29161 aatgcatcct gtgacattgt cacttcgcgt gaaagccagg tcacggcgta ctgttccggc
    29221 ggcggcgcaa ggtaatgtaa ccgacaacga ctacgtaatg ttgccagtaa acgttcaggc
    29281 tcgcgggtag ccaggaaaaa ccaggtttct gctggtggct cttcaagcgt tttcagcaat
    29341 gcgttagccg cggcgtcggt cagtaaggca gcatcggtta cccagacaac tttcgcacca
    29401 cctaggcgtg cgtgctcatt cagcttttcg gtgacctcac gtaccgcatc aatgcccagc
    29461 atatttttcc ctttttcggg agccagggtg tagtaatcgg gatgcgtgcc agcctgcatc
    29521 aactgacatc cacgacagtg accgcaactt ttgtggccct gcggttgttg gcagagtaaa
    29581 taacggctca gggcgtagat taaagcatca tcgcccatgc ccggtaacgc ctgaatgagt
    29641 agcgcatggt gacctcttcc ggcctgatag ctggctacca gtttttcgaa atcaggtcgt
    29701 aaccatggat accatctcat gcgtccaact ccttaaccca gtgggtcacg gtagtgcgga
    29761 ttgcatccat cacggcctcc agcggctggg tggcatcaat ggtatgaata cttttatctt
    29821 gtgcagccag ttccagatag cgggcgcggg tgcgattaaa gaaatcgaaa gactcttgct
    29881 caatacgatc cagctcgccg cgcgcacgcg cgcgttttaa gccaacttcc ggggtaacat
    29941 cgagatagag cgttaagtcg gggcgaaaat ccccgagaac agcatcacgc agtgttgcca
    30001 gcatatgttg gtcaatacca cgtcctccgc cctgatacgc ctgagtggag agatcgtggc
    30061 gatcgccaat cacccaggtg ccgttagcca gagctggttt gatgaccgtt tctaccagtt
    30121 gaacgcgcgc ggcataaaac atcagaactt cggctttatc ggtaatgact tcatcgccta
    30181 ccgatttgat atccagcacc aggcttctta acttttcggc aagttgcgta ccgccaggtt
    30241 cccgagtgaa aaccatgtcg cggataccca gttgctcaag cgtctcaacc accacattac
    30301 gcgcggtagt tttgcctgcg ccttccagcc cctcaatgac gatatactta ctgcgcattt
    30361 ttttccttaa gcactttcag ataatcctgc acagacttgt tatggctggc aagattggta
    30421 ttaaacgtgt gaccaccttt accatcggcc acaaaataga gatacggcgt ttttgccgga
    30481 tgcgcagcag ccttcagcga atccgccccc ggcgtagcta tcgcacccgg cggcaaaccg
    30541 gtaatggtat aggtgttata cgctgtcggc gtttccaggt ctgcacgaga aagtttgcca
    30601 ttataacgct ctcccatccc gtaaatcacg gttgggtcgg tctgcaggcg cataccaatg
    30661 cgtaaacggt tgataaatac cgaggcaacc tgatcgcgtt cactggcaac ggcggtttct
    30721 ttttcgataa ttgatgccat cgtcaccagc tgatttttat ctttataagg cagaccgtcc
    30781 gcacgccctt cccaggcgct atcgaccgct ttcaccattt tcttgtgcgc tcgcttgagt
    30841 aacgcgacat cggtggtatt ggcggtatac atccaggtgt ctggccagaa ccaaccttca
    30901 atccactccg ggttttccag ttcaagtgcc tgcgctacgg tggcgtactt atcgtcactc
    30961 agcgtgtgct tgatatacgg agcctcacgc aattgcttga ggtagtcgct cagacgcatc
    31021 ccttctacca gtcgcagcgg gaactgtgct tctttaccgc tttccaacaa tttcagcatc
    31081 tcgcgcacgg tcatctgcgg tgtaaagcgg taagtcccgg ctttaaagtg agaaagatcc
    31141 ggttcgatac gcagcagcca ttgaaaaacc cgtgggcgat tgatgatctt atcagcataa
    31201 agctgttcac cgagcgccag acgtccggtc cctggcttca gggtaaatat cgtctcttct
    31261 ttgataagca atttgctgtc ggcaagatgg cgaaccttcc agacgcccac accagcggcg
    31321 atacccagta ccaccagcaa taacaagata atcaataaca cttttttcat gactaattcg
    31381 ggcgctcaca aagtggggct aaatattcat ataacgttgc tgacgaaaag gagacatcgc
    31441 cacaggcacg tacgggcatc actggcatta acgcattaca aataaccatc tcatctgcct
    31501 gcaacgcctc ttccagagag gcttgcactt cgacaagctg ataagaggat tgtgccagca
    31561 aacggataca gaattgtcgc ataatgccgt taacacctgc ctgatccagt cgcggcgtat
    31621 aaactacatt gcccttccgc cagaacaaat tagccgcaca gcattccgta acccaccctt
    31681 cgctgtcaag gaccagcgcc tcatcagcgt ttgtctgctc aagatgagag cggatcaata
    31741 cttgctcaag ccgattaaga tgtttaatac ctgcaagatg aggattgcgc cccagccgca
    31801 ccgggcttag cgccaacgtc atcccttcgt tacgcaaacg gtcgtaatgt gcaggataaa
    31861 ccgtaacgga gagaatccgc gttgccggtc cactgttcaa tgtgctgtac cctcgcccgc
    31921 cactaccgcg actgatcacg actttcagca caccattttg ctgttctgct gccagcgttt
    31981 tcatctcttg ttcaagctga ggccagaagt cacagaaaat catcaaccgc tgacaagcat
    32041 cctgtagtcg ctggatatgc gccgataaca aactgacttt accgtcgata actctggcgg
    32101 tggtaaaaca accatcacca aactgcgttg cccgatcgct tactgccagc gattcctgct
    32161 tataaccgtt aattaagaac ataccggctc cttatcagag aattgttagt gtggcagcat
    32221 gttcactacg gaacaagtcg gaataaaagc taagaaaaaa ggcccgcaag cggacctttt
    32281 atatgggtgg aaaatgacaa cttagatctt tttaaagatc aaagaaccat tagtgccacc
    32341 gaagccgaag gagttacaca gcgtgtattc cattccgctg acctgacgtg cttcatgcgg
    32401 tacgaaatcc agatcgcaac cttcatccgg gttatccagg ttgatggttg gcggaacagc
    32461 ctgatcgcgc agcgccagga tggagtagat agattctact gcacccgccg cacctaacag
    32521 gtgaccggtc atagatttcg tggagcttac caacacacgg cttgcagctt caccgaagat
    32581 ggttttcacc gcctgcgctt cagctttatc gccagccggc gtagaagtac cgtgcgcgtt
    32641 aacgtagcca atctgactcg cttcaatgcc tgcatcacgc agagcatttg ccattgccag
    32701 agctgcgcct gcgccatttt ctggcggtga cgtcatatga taagcatcgc tgctcatacc
    32761 aaagccgacg agttcagcgt aaattttcgc accgcgtttt ttcgcgtgtt cgtactcttc
    32821 aagtaccagc ataccggcac catcgcccag tacgaagcca tcacgctctt tatcccacgg
    32881 gcggctcgcc gcttgcgggt tatcattgcg ggtagataat gcacgtgccg cgccaaaacc
    32941 accaacgccc agcggcgtac tggctttctc tgcgccacct gcaaccatca cgtcagcatc
    33001 gccatacgcg ataatacgcg cagcatgacc aatgttgtgc acgccggaag tacaggcggt
    33061 cgcgatagag atgcttgggc cacgcaggcc atacatgata gtcagatgac ctgccaccat
    33121 gttcacaatc gttgacggaa cgaagaatgg gctgatctta cgtggaccac cgttcatcag
    33181 agatgtgtgg ttttcttcga tcagtccgag gccgccaatc ccggagccaa ttgcggcacc
    33241 aatgcgggtt gcgttctctt ccgttatttc aaggccagaa tcctgcatgg cctgaacgcc
    33301 agcgacaatt ccatattgaa tgaaggcatc catcttgcgc tgttctttgc gcgagataat
    33361 gtcctcacag ttaaaatcct ttactaagcc agcaaatttc gttgcatagg cgctagtatc
    33421 gaaatggtcg attaggctga tgccactctg accggcaagc agagctttcc aggtagactc
    33481 tacggtattg ccgacaggag acaacatgcc cagtccggtc acaactacac gacgcttaga
    33541 cacgtttgtc ctccagggag ggaaaaaatg attctagtgg gacaaaaaga taaaactcag
    33601 gcggtcgaac gaccgcctgg agatgttcac ttacgcctgg tggccgttga tgtaatcaat
    33661 ggcagcctga acggtggtga ttttctcagc ttcttcgtcc ggaatctcag tatcaaactc
    33721 ttcttccaga gccattacca gctcaacggt gtcaagagaa tccgcgccca ggtcttcaac
    33781 gaaagaagca ttgttggtaa cttcttcctg cttaacgccc agctgttcgc cgataatttt
    33841 cttaacgcgt tcttcgatag tgctcatact cttaaatttc ctatcaaaac tcgctttcgc
    33901 gatggttttc gtagtgtata aaatgttgaa aaatttgcaa ctaaatcccg gcaggtctta
    33961 ccacgatttt acgttatttt gaggccttac cccccaataa cgcaaatcat tttgcactaa
    34021 ttattactca taaccacgca gactgcgcgc aacatgagca ttttgtgcaa atcgcggtca
    34081 gaccatgtac atcccgccgt tcacatgcaa agtttcaccc gtgatgtaag ctgcttcgtc
    34141 ggatgccagg aatgcaaccg cgttggcgat ttcctgtgcg ccgccgaggc gacccgcagg
    34201 aacctgcgcc aggatacccg cacgctggtc atcgctcagc gcacgtgtca tgtccgtttc
    34261 aataaagccc ggagcaacaa cgtttacagt aataccgcgt gacgcaactt cgcgcgccag
    34321 tgatttactg aagccgatca agcccgcttt cgccgcagcg tagttggcct gaccgccatt
    34381 tcccatggta ccaaccacag aaccgatagt gataatacga ccatgacgct ttttcatcat
    34441 agcgcgcatt accgcttttg acagacggaa aacagatgaa aggttggttt cgataatttc
    34501 gttccactct tcatctttca ttcgcattaa caggttatca cgagtgatac cggcattatt
    34561 gaccaggata tccacttcac caaattctgc gcgaattttt tccagaacag attcgataga
    34621 tgccgggtcg gtcacattca acatcagacc tttgccgttg gcacctaaat aatcgctgat
    34681 cgcctgagcg ccattttcac tggtcgcagt gccaataact ttcgcgccac gggctgcgag
    34741 cgtttcagca attgcgcggc caattccgcg gcttgcaccg gttaccagtg cgatttttcc
    34801 ttcaaaattc atgattttcc tcttttaaag ctcgagcgcc gctgccatcg ctgaaggttc
    34861 gttcagcgcc gaggcggtca gggtgtcgac aatgcgtttc gtcaggccag taagcacctt
    34921 gcccgggccg acttcataga gatgttctac gccttgcgct gccatgtact caacagactt
    34981 cgtccactga accgggttat acaactgacg taccagtgcg tcacggatgg catcaccatt
    35041 ggtttcgcat ttcacatcaa cgttattcac aacagaaact gttggtgcgt taaaggtgat
    35101 tttcgctaat tctactgcca gtttgtcggc tgctggtttc atcagcgcac agtgagacgg
    35161 tacgctcact ggtaacggca gcgcgcgttt tgcgccagca gctttacagg cagcgccagc
    35221 acgctcaacc gcttctttat gaccggcaat aaccacctgt cccggagagt taaagtttac
    35281 cggagaaacg acctgacctt ctgcagcttc ttcacacgct ttcgcaatag acgcatcatc
    35341 cagaccgatg attgccgcca tagcgccagt gccttccggt acggcttctt gcatgaactt
    35401 accgcgcatc tcaaccagac gcaccgcatc agcgaaatta atcacgccag cgcaaaccag
    35461 ggcggagtat tcccccaggc tgtgaccggc catcattgcc ggtgctttac cgccctgctg
    35521 ctgccatacg cgatacagcg caacagatgc aatcaacagc gctggctggg tttgccaggt
    35581 tttattcagt tcttcagctg gcccctgctg ggtcagcgcc cacaggtcgt agcccagcgc
    35641 cgcagaagct tcagcaaacg tttcttcgac aattggatag ctcgccgcca tatcagccag
    35701 cattccaacg gtttgagaac cctgtccagg gaacacaaat gcaaattgcg tcatgtttta
    35761 atccttatcc tagaaacgaa ccagcgcgga gccccaggtg aatccaccgc caaaggcttc
    35821 aagcagaacc aactgccccg gcttaatgcg cccgtcgcgt acagcttcat ccagcgcgca
    35881 cgggacagag gccgcagagg tattaccgtg gcgatccagc gtcaccacga cattgtccat
    35941 agacataccg agttttttcg ccgttgcact gataatacgc aggttagcct gatgcggaac
    36001 cagccagtcc agttgagaac ggtcaagatt atttgccgcc agcgtctcat caacgatgtg
    36061 cgccagttcc gttaccgcaa ccttgaagac ttcgttgccc gccatcgtca gatgaattga
    36121 attctctgga ttcacacggt cagcattagg cagcgtcagc aactcaccat agctaccgtc
    36181 ggcatgcaga tgggtggaga tgattcccgg ctcttcagag gcagccagca ccgcagcgcc
    36241 cgcgccatcg ccaaaaataa taatagtccc acgatcggtt ggatcgcagg tgcgcgccag
    36301 tacatcggaa ccgacgacca gagcatactt caccgcccca gatttcacgt attgatcggc
    36361 tacgcttaat gcataggtga aacctgcgca ggctgctgca acgtcaaatg ccgggcaacc
    36421 tttaatgccc aacatgcttt gaatctgaca agctgcgctc gggaaagcgt gcgtagcaga
    36481 agtcgttgcc acaacgatca ggccaatctg gtctttctca atgcccgcca tctcaattgc
    36541 gcgtgtcgcc gcttcaaagc ccatggttga aacggtttcg tttggcgcgg caatgtggcg
    36601 ttcgcggata ccggtacgag tgacaatcca ctcgtcagag gtgtccacca ttttttccaa
    36661 atcggcgttt gtccgcactt gttcgggcag atagctgcca gtaccaataa tcttcgtata
    36721 catgtacgct cagtcacttt tcggttatat accgtcactt gcaaactgcg agttcgctgg
    36781 cagcgtcctg ctaccgcaga gttccgcttt tgccaccgtc cagcagctca aaaccagctg
    36841 ggtatacaga ttccaggcga gcggcaattc gctgaggaac ttgtcgctgc accgcctgca
    36901 ctgcctgttc aatcgcgacc gcaaaagctc gctgattggc tgcaccatga ctttttatca
    36961 ccgtgccgcg caatcctaac agacaggcgc cgttatactg gtcggggttg aggtgactga
    37021 atcgcctcgt caggctcttt tgtagccaac gctttaataa cagtagccac cacgaccgtt
    37081 ttttcccttc accctgagat ttcagcagag aaaggaacat cctgacaaca ccttccatcg
    37141 tctttaacgt gacatttcct gtaaagccgt cacaaaccag cacatctgtc ttgccagtta
    37201 acaactcatt ggcttcaaga tagccgatat aattgataga agggattgtt ttaagcaccg
    37261 ctgaggcatc ccgaatactg tcgagaccct ttacttcttc ttcaccaata ttgagcaacg
    37321 ccacgcgagg attgggaatt tccaccacct cttcagccag aactgagccc ataatggcaa
    37381 attgcaccaa cattgtgctg tcacaatcga cgttggcccc taaatcgagg accaccgttt
    37441 tgcccttttg ctgatgtggt aataccgtca ccagcgccgg acgctcaatc ccctccaggg
    37501 gcttgagtaa taattttgcc agccccatca gtgccccggt attaccggca ctgacacagg
    37561 cttgcgctcg accttctttc accagctcca gggccatacg cattgaactc ccacgactgg
    37621 cgcggatagc ttgcgaaggc cgggcatcac tggcgataac tgactgcgca ggaataatct
    37681 gcagacgcga acgttgttca aagtcagctt tagcaagtaa tggcgtgatg gcgtcgggat
    37741 tgccgactaa aagaagagtg agttgcgaat tagaattcag tgcctgcaat gctgcaggca
    37801 ctgtcacgga agggccaaaa tcccctccca tgacatctaa cgccagggtt agacgtgtca
    37861 aggtatcgtc gccgcccggt ttggtctttc cccagttgcc tggggaaaat cctcactaag
    37921 cttcatcacg cagacgcgtg attacttagc gatgaccttg cggccgcggt agtaaccgtc
    37981 ggcagtgatg tggtgacgca ggtgtttttc accagaagtt ttgtctacag acaggctggt
    38041 gactgcggtc agcgcgtcat gggaacgacg catgccacgt ttggaacggg ttggtttatt
    38101 ctgttgtacg gccatggacc ttactcctca attacggttt atccccgatc caacggggag
    38161 caccaattac ttacgcttta agctggctaa tacggcaaat gggtttggct tttgcgcttc
    38221 ttcaggcagt tcaccaaaga ccatgtccgc ttcggacact tcacagtgtt cagaatcatg
    38281 caccggaact accggcaagg cgaggatgat ttcatcttca accattgcaa gcagatcgat
    38341 ttcaccgaat tcgttaacct caatcggttc atacgcttcc ggcagtgctt cagcctgttc
    38401 gtctgaacgc acaggactaa aacaatacgt tgtgtagacc tgatgagtaa acggcttccc
    38461 gcaacgctga cactcgagcg ttaccgtcac cttcgcatcg ccgtttaaca ctgcgagacg
    38521 ttggttatcg atagcgaacg acatggagca ttccacatca ctgtccacac tgactacgga
    38581 ttcggcgacg cgctcaacct gatcaggggt atagataccc tggtaatcaa ggcgtttttg
    38641 agccgtacga accggatcga gagtcagggg taattttacc ttttgcatag ggcgcgcata
    38701 ttaactttgt aacgtcatag agtcaaagaa aaaggcagcc tgtggttgcc ttttgccaat
    38761 aattcgcaca cattgcgggt tactgcttta ttttcgttca gtgctgataa cgaacatcaa
    38821 cctgcaaaac gaagcgcata gtttaaaatg gctatcatca atacgctata tctgatggaa
    38881 aaaaatatgc ctaaacttat tttagcctcc acctcgccat ggcgtcgcgc tcttctggaa
    38941 aaactgcaaa tctcttttga atgtgcagca ccagaggtcg acgaaacccc acgcagcgac
    39001 gaatcaccgc gacagttggt gcttcgactg gcacaagaaa aagcgcaatc tctggcgtca
    39061 cgttatccgg atcatttaat tattggttca gaccaggtat gtgttcttga tggcgaaatc
    39121 accggtaaac cgttaacgga agaaaatgcc cgtctgcaat tacgcaaagc cagcggcaat
    39181 atcgtcacct tctatactgg actggcgttg tttaattcgg cgaatgggca tctacaaaca
    39241 gaagtggagc cttttgacgt ccatttccgt cacctgagcg aggcggagat tgataattac
    39301 gtgcgtaagg agcatcccct gcactgcgcg ggtagcttta agagtgaagg atttggcatt
    39361 acgctgtttg agcgtttaga gggacgtgac cctaacacgc tggttggttt accgctaatc
    39421 gcgctgtgtc agatgttacg tcgggaaggg aaaaacccgc tgatgggata gttgtttgcg
    39481 ccggatgcgg ttacgtcgca tccggcaatc ggtgcaggcg gcctgatgcg gcgtaaacgc
    39541 cttatcaggc caaccgccca ttatcaggct tatatgcttg tattagcgcg cgttacgcag
    39601 cttttgcaaa caacgcttca aaccttcatc catcggcgct tcgatacgca tcacctcacc
    39661 ggtccccgga tgggtaaact tcaacgctgc ggcgtgcagg aacagacgat ttaatcccgt
    39721 gcctgcttca gtgagctgtc tgtcaaattc acggtcaccg tagcgatcgt caaaggcaat
    39781 cggatgaccc gcatactgtg tatgcacacg gatctgatga gtacgtccgg ttaccgggct
    39841 acaacgcacc agggtggcaa atgcatagcg ttcttccact ttaaagcgtg tttccgacgg
    39901 tttgccttcc tgactgacac gcacgatacg ttcgccgctt tgcagaatat ttttcaataa
    39961 cggcgcttga acgctcttca catgcgactg ccactgaccg cgcaccagcg ccaggtaatc
    40021 tttttgcatt cctttttcac gtaattgctc atgcagagaa cgcaacgccg agcgtttttt
    40081 cgctaccagc aacacacctg aagtgtcccg gtcgagacga tgaaccagtt caaggaaccg
    40141 cgcttccggg cgcaacgccc gcaaaccttc gataacgccg aagcttaaac cactgccgcc
    40201 atgtaccgcc gtaccggaag gtttattcag caccaggata tgatcatctt catataagat
    40261 gacgtccgcc agcgccgcca ccttttgcag atgtggcgaa accgcctctt cttcccgctc
    40321 agcaacgcga accggtggga tacgcacctc atcacccgct tcgagtttat attcaggctt
    40381 aatacgtttt ttgttcaccc gcacttcacc tttacgcaaa atacggtaaa tcatactttt
    40441 tggtacgcct ttcaattggg tacgcaaaaa gttatcgata cgttgccccg cttcgtcagc
    40501 ggtgatagca acaattttta cggatggagt ctctgttttc atggtgggcg attctaatta
    40561 gccaacagga ttcgcgccac tcatttttct atgcttatat ttagttttac accttattac
    40621 ttcactacgt gatcacttta ttgatggtta ttaaaccaat caccagcaag aagtgaaaaa
    40681 actgtgagta agcgggtgat aaatggtaaa agtcatcttg ctataacaag gcttgcagtg
    40741 gaataatgag gccgtttccg tgtccatcct tgttaaaaca agaaatttta cggaataacc
    40801 cattttgccc gaccgatcat ccacgcagca atggcgtaag acgtattgat ctttcaggca
    40861 gttagcgggc tgcgggttgc agtccttacc ggtagatgga aatatttctg gagagtaata
    40921 cccagtctgt ttctttgata attgcgctgt ttttccgcat gaaaaacggg caaccgacac
    40981 tctgcgcctc tttgagctga cgataaccgt gaggttggcg acgcgactag acacgaggcc
    41041 atcggttcac acccggaaag gcgttacttt gcccgcagct tagtcgtcaa tgtaagaata
    41101 atgagtaagt tacgatgaaa agaatgttaa tcaacgcaac tcagcaggaa gagttgcgcg
    41161 ttgcccttgt agatgggcag cgtctgtatg acctggatat cgaaagtcca gggcacgagc
    41221 agaaaaaggc aaacatctac aaaggtaaaa tcacccgcat tgaaccgagt ctggaagctg
    41281 cttttgttga ttacggcgct gaacgtcacg gtttcctccc actaaaagaa attgcccgcg
    41341 aatatttccc tgctaactac agtgctcatg gtcgtcccaa cattaaagat gtgttgcgtg
    41401 aaggtcagga agtcattgtt cagatcgata aagaagagcg cggcaacaaa ggcgcggcat
    41461 taaccacctt tatcagtctg gcgggtagct atctggttct gatgccgaac aacccgcgcg
    41521 cgggtggcat ttctcgccgt atcgaaggcg acgaccgtac cgaattaaaa gaagcactgg
    41581 cgagccttga actgccggaa ggcatggggc ttatcgtgcg caccgctggc gtcggcaaat
    41641 ctgctgaggc gctgcaatgg gatttaagct tccgtctgaa acactgggaa gccatcaaaa
    41701 aagccgctga aagccgcccg gctccgttcc tgattcatca ggagagcaac gtaatcgttc
    41761 gcgcattccg cgattactta cgtcaggaca tcggcgaaat ccttatcgat aacccgaaag
    41821 tgctcgaact ggcacgtcag catatcgctg cattaggtcg cccggatttc agcagcaaaa
    41881 tcaaactgta caccggcgag atcccgctgt tcagccacta ccagatcgag tcgcagatcg
    41941 aatccgcctt ccagcgtgaa gtccgtctgc cgtccggtgg ttccattgtt atcgacagca
    42001 ccgaagcgtt aaccgccatc gacatcaact ccgcacgcgc gacccgcggc ggcgatatcg
    42061 aagaaactgc gtttaacacc aacctcgaag ctgccgatga gattgctcgt cagctgcgcc
    42121 tgcgtgacct cggcggcctg attgttatcg acttcatcga catgacgcct gtacgccacc
    42181 agcgtgcggt agaaaaccgt ctgcgtgaag cggtgcgtca ggaccgtgcg cgtattcaaa
    42241 tcagccatat ttctcgcttt ggcctgctgg aaatgtcccg tcagcgcctg agcccatcac
    42301 tgggtgaatc cagtcatcac gtttgtccgc gttgttctgg tactggcacc gtgcgtgaca
    42361 acgaatcgct gtcgctctct attctgcgtc tgatcgaaga agaagcgctg aaagagaaca
    42421 cccaggaagt tcacgccatt gttcctgtgc caatcgcttc ttacctgctg aatgaaaaac
    42481 gttctgcggt aaatgccatt gaaactcgtc aggacggtgt gcgctgtgta attgtgccaa
    42541 acgatcagat ggaaaccccg cactaccacg tgctgcgcgt gcgtaaaggg gaagaaaccc
    42601 caaccttaag ctacatgctg ccgaagctgc atgaagaagc gatggcgctg ccgtctgaag
    42661 aagagttcgc tgaacgtaag cgtccggaac aacctgcgct ggcaaccttt gccatgccgg
    42721 atgtgccgcc agcgccaacg ccagctgaac ctgccgcgcc tgttgtagcc ccagcaccga
    42781 aagctgcacc ggcaacacca gcaactcctg cacaacctgg gctgttgagc cgcttcttcg
    42841 gcgcactgaa agcgctgttc agcggtagtg aagaaaccaa accgaccgag caaccagcac
    42901 cgaaagcaga agcgaaaccg gaacgtcaac aggatcgtcg caagcctcgt cagaacaacc
    42961 gccgtgaccg taatgagcgc cgcgacaccc gtagtgaacg tactgaaggc agcgataatc
    43021 gcgaagaaaa ccgtcgtaat cgtcgccagg cacagcagca gactgccgag acgcgtgaga
    43081 gccgtcagca agctgaggta acggaaaaag cacgtaccac cgacgagcag caagcgccgc
    43141 gtcgtgaacg tagccgccgc cgtaacgatg ataaacgtca ggcgcaacaa gaagcgaagg
    43201 cgctgaatgt tgaagagcaa tctgttcagg aaaccgaaca ggaagaacgt gtacgtccgg
    43261 ttcagccgcg tcgtaaacag cgtcagctca atcagaaagt gcgttacgag caaagcgtag
    43321 ccgaagaagc ggtagtcgca ccggtggttg aagaaactgc cgctgccgaa ccaattgttc
    43381 aggaagctcc agctccacgc acagaactgg tgaaagtccc gctgccagtc gtagcgcaaa
    43441 ctgcaccaga acagcaagaa gagaacaatg ctgataaccg tgacaacggt ggcatgccgc
    43501 gtcgttctcg ccgctcgcct cgtcacctgc gcgtgagtgg tcagcgtcgt cgtcgctatc
    43561 gtgacgagcg ttatccaacc cagtcgccaa tgccgttgac cgtagcgtgc gcgtctccgg
    43621 aactggcctc tggcaaagtc tggatccgct atccaattgt acgtccgcaa gatgtacaag
    43681 ttgaagagca gcgcgaacag gaagaagtac aagtgcagcc gatggtgact gaggtccctg
    43741 tcgccgccgc tgtcgaacct gttgttagcg cgccagttgt tgaagaaatg gctgaagtcg
    43801 tagaagcccc cgtaccggtt gccgaaccgc aaccggaagt ggttgaaacg acgcatcctg
    43861 aagtgattgc cgccgcggta actgaacagc cgcaggtgat taccgagtct gatgttgccg
    43921 tagcccagga agtcgcagaa cacgcagaac cggtggttga accgcaggaa gagacggcag
    43981 atattgaaga agttgccgaa actgctgagg ttgtggttgc tgaacctgaa gttgttgctc
    44041 aacctgccgc gccagtcgtc gctgaagtcg cagcagaagt tgaaacggta gccgcggttg
    44101 aaccagagat caccgttgag cataaccacg ctaccgcgcc aatgacgcgc gctccggcac
    44161 cggaatatgt tccggaggca ccgcgtcaca gtgactggca gcgccctact tttgccttcg
    44221 aaggtaaagg tgccgcaggt ggtcatacgg caacgcatca tgcctctgcc gctcctgcgc
    44281 gtccgcaacc tgttgagtaa taattagctc aaagtaatca agccctggta actgccaggg
    44341 cttttttatt tcatctttga atcaacatat taaaattcat caaatggcat ccttgctaac
    44401 caacaatgca aaataggcaa aaaaatgccc gccccggcga tcccagagca ggcagacaaa
    44461 acatacccag tttcatgata tgttttaaag cgagcttatt tgttgagctg gaagagcgac
    44521 aatccctgca tatcggtaaa tgctttatac gatgcctgca atgccgtttg ctgcatgatg
    44581 taagatgaaa tagttgcatt ccagtccaca tcaaccagat cgctcatctg ctgcgtttgc
    44641 cctaaagcgc gatcgctacc taatgaatcc agcgactcca gttcgttcag ctgcgtgcct
    44701 aattccgcgc gaactgtaag gacattattc agcgagtttt tcagtccacg gttggtttta
    44761 tccaatgccg cagcggcgat ttctttatcc gcttcgctat ccgcgaccgg cgttttcagg
    44821 gctgcgatgg cgctatccag catggcaaaa agattggttt cagaagcgct accgtctggt
    44881 tccgctaccg cgttactggt aatactgtcg aaaattttgt cgcccgtatg tcctatcacc
    44941 atcgaacgcg aagcatcgac ctgttgccta atactttctg ctccaccgac gtatttccct
    45001 ttctcttcgc taaacggcgc agtctctgtt ttataaccgg caaaaatgta gcgcccgtta
    45061 ccgtcagtgg tgttcgccag attcagcaac tggtcacgaa gcccctggat atccgtagcc
    45121 agcgaggccc ggtcatcgtc actcaaagtg ccattgctgg cgtagacaat tttttcctga
    45181 gcattctgga tagcagtggt ggcctggcta agtacactct cttccagtga cactttttga
    45241 gtggcgaaag tacgcgccag cgtgtactgg ctgttttgcg cctgtgcctg ggagagaaca
    45301 accgcttgtg atgctgcaat gggatcgtca gaagggttaa cgactcgctt acctgtcgac
    45361 atctgctcgc cgtacttcat ccattccgcc tgagaattgg tgatgccacg catgttttgc
    45421 tggtacatca tctgggtact gaaacgcatc ttatctcccc ttagcgaatg ttaatcagcg
    45481 catcaaaaat cgcgtttgcc gtctgcagaa cctgcgcatt cgccaggtaa tactgctgaa
    45541 aacgttgcag atttccgtac tcctcatcga gattgacacc ggaaatcgac tgctgctgat
    45601 tggaaagctg cgtcaccaca ttaccttgcg tggcgctact ggttttcaac gtcgcggttt
    45661 tattaccgat atcactcact aacgaggcat aagcgtcgtt aaaggatttc gcaccgccca
    45721 ccgttttact gttgctttgc agatccagca gggcctgacc gttgcggtta tcgctatcac
    45781 ccgcatcttc ttcgctcgcc atcgctattt tcgcttcgtc ggtgattaat acatccatgt
    45841 tgacgatagc gtcacttact ggtttcagcg tgaagctgtc gttaacagca ggcgttcctg
    45901 taaacgtcaa ctccagacca tcaaatgcca ctttaccgtt ggcatccggc gtcaccgtaa
    45961 aagtggtatt actggcaagg cgggtgacct gccactgatt attatcgaac gagattttgt
    46021 aatcagtcgc cagtaccgcg gaggcatcag ttaccgtggc accgatcgca acgtcacctg
    46081 tgtttttcgt gttttgcaga accgcgggct taccgatagc aaagaaatct tcaccagcat
    46141 cgccgttggc atcaaatccg gctttgtgtt ggctgttgaa agcctcggca aatgccagcg
    46201 ccagttgtcc aagcgtatta cgcgtctggt ccagatcctg agaacggaat gtcagaatgc
    46261 cgcccagcga cccggtattc agtaatttct ccgggatctc aatattgcct gccgtcccat
    46321 caacgtaagc gacagtcgta cgagaagggt cggcgctgga aggaactgcc gccagttgcc
    46381 gcgccgtact tccctgaacc agtgagtaac cattggccat cgtgatgtta taagtaccgc
    46441 catcctgaac gctgacttct acgccaacaa tctggtttaa ttcgctcacc agttgatcgc
    46501 gttgatccag cagattgtta ggtgacgccc ctgcccccac gcctgtcagg cgcgagattt
    46561 gatcgttcag gctggcaatt tgtttagcgt aattgttgat ctgatcaacg ctggcaccta
    46621 tcgcgatatt gacctgtttg tcctggtcgc gcagatattg atcggtggtt ttaaactgat
    46681 tcaccaaccc ttctgatttc ccaatcagcg cctggcgcgc tgccgggtct tccgcgttac
    46741 tcaccagcgt ttgcaggctg gtgaagaaat cctgcatctg tgttgccagc gaagaggtac
    46801 tggtggagag catattgtcg attttcgaca tctgctcata gcgggcagtc aggccgctac
    46861 tttgcgtctg cgccgcacgt aactggttgg taataaaagc atcatactca cgctgcacac
    46921 cagaaacgta gacaccattg ccaacccagc cgccagcgcc caacgtgcta ttggcctgcg
    46981 ccataatagt ggtttggcgg gtatatccgg caacgttata gctggagata ttattacttg
    47041 ccgtatttaa cgccgcctgg gccgcgttca gtccgctcat ggcgttattg atcaagctgg
    47101 acatggaggt tccttataag ccttcaatta cagagtatta tcggcagcga cccgccggac
    47161 ttgagttatt cagaacagat tatcaatgtt catgctgtag gttttgctca ccttgtcgct
    47221 tatcgatttc atctgctgga tcatgttggt gagtttacgg gcatagtgag gatcggtagc
    47281 ataccccgcg tcctgtaggg cctgcgcccc ctgctccgca ctcgcggcgg tcgtcacggc
    47341 ggcgtagcgc gggttacgtg ttaacagccc aacgtaatcc gacaatgctt ccagatacga
    47401 gctgtagacc cgaaacttcg cttttacttt cttcgcttcg ccgttttcat attcagtcgt
    47461 ggtgatttca gtaactggcc ctttccagtt gccagaggct ttgacaccaa acaggttata
    47521 gctcggctcg ccgttttcgc ggcggatttg ccgttgcccc caaccagatt ccagtgccgc
    47581 ctgagcgagg atcaaatgat gtggcacacc gctttgctgg cttgccagtt gggcgggcag
    47641 cgagagttgc gcgaggaatg ctttactgtc acccggcagc gaatcatcgt agttacgtgg
    47701 cacggccttt tgcaccagct gcgaaagcgc ctgattttga taacgcacca cagtttcgag
    47761 cgggaatttc atcggtgctg ctggcgtgga ctcctctggc aatggttgtt ctggcgtcat
    47821 ctgtttaacc atcatctctg caagccccag acctttgccc gccgtcatct gttgggcaat
    47881 ctgctggtca tacatactgg tatacaggcg agtgtgctcg ctgctgaaca ggccatcttt
    47941 tggtaaagcg tcgcgcatgc ttttcaacat catctgcacg aacatccctt ccacctgacg
    48001 ggccaccgga cggatatttg ccgccggatc ttcgcccgct ttcgccttta gttcgttgag
    48061 tgattgcgca tcccaggccg cacttgccag tagtttgctg tcgctgatca tcagatgatt
    48121 tccagttttg cccgcagaca tcccgcactt tgcattgatt gcagtatgga catcagatcc
    48181 atcggcgtag cgcccagcgc attgagcgcg cgcaccacgt tattgaggct ggcgctggaa
    48241 cgtacgcttt gcagcgaacc gccgctctgg cgtaaatcga tctgcgtttg tggagtaacc
    48301 acagtctgtc caccaccaaa cggtgtatct ggctggctga cattggcctg acgattgact
    48361 gttactgaga gatttccctg cgctaccgcg cagctgtcga gggtcacttc gcgattcatc
    48421 accaccgaac cggtgcgcga gttaatcact actttagcgt cctgcggggt gacattaacc
    48481 tgcatattct ggatatctgc aaggaaacgg acctgggaac tgttgccact tggtacgcgc
    48541 acctgaatag tccgcgcatc taacgcggtg gcgctgccat atccacgcac gcggttgatg
    48601 gtgtcagcga tttgctgcgc catgctgaaa tcttcgtcgt taagttgcaa attaagggta
    48661 ttcccgacgc caaactggct gggcaattca cgttcaataa ccgcaccatt ggtgatccgt
    48721 ccaccgttca gttggttcac ctgaacactg ctaccgccag cggaggctcc tgcgccgcca
    48781 accagaatat tgccctgcgc cagcgcatac acctgactgt caacgccctt aagcggcgtc
    48841 atcaacaacg tacctccacg caagcttttg gcatttccca tggaagaaac caccacatcg
    48901 atggtttgcc cctgacgtcc aaacggagga agtgacgctg tcaccattac cgcagcgacg
    48961 ttttttagct gcatattggt gcccgtcgga acggtaattc ccagctgtga gagcatgtta
    49021 ttaagcgttt gtgtggtaaa cggcgtctgg gttgtctggt caccggtgcc atccagcccc
    49081 accaccagac catagccaat cagtgagttt tgccttaccc cctgaacact ggtgagatcg
    49141 cgaatacgct cagcctgagc cgccgtcgta accagtagaa gaattaatgc agagagaaat
    49201 ttaatcacca cagcctcact tacattggcg acaggttaag gaagaaacgc tgcaaccagc
    49261 ccatattttg cgcttcgtta atgtagccat tgcctacgta ttcaatgcgc gcatccgcca
    49321 cctgagtaga cggtacggta ttgctgccgc tgatagtgcg tggattaacc acgccagaga
    49381 agcgaataaa ttcggtaccc tgattaatgg caatctgttt ttcacccacc acatgcaggt
    49441 tgccgttgac cagtacctgg tcaaccgtca ccgtcaacgt gccgctaaag gtattgctgg
    49501 cattggcccc gccctttccg ttgaacgtgt taccaccgga ggcttcgaca tcggcacgag
    49561 cgttaccaaa cagcccctgc aaatagcgcg gcacagtatc aaagccaaaa ttagttttac
    49621 cgtcacggct ggcattcgca gaggagcttt tgctggcgct gacgttctcc tgcaacacga
    49681 tggtcagcgt atcgccaata ttgcgtggtc gacgatcttc aaacagcggt tgatagccat
    49741 agttaatcgg ctgagcagac tggaaaatag aaccgttggc gacgggcgtc ggcccgggaa
    49801 ccggttgtgc actggtcgcc ccctgcacca gcggcgtgga gggtatccag gcgcagccgg
    49861 ttagtgaaag caccaacaag ctggaaatgg cataagtatg cgcagcgttt ttttgcatgg
    49921 ctatcatctt caaaaatcag taaaccggtg aacctgccac cggttaagcc ttagagttgc
    49981 gtcagttttt gcagcatctg atcggtggtg gacaccgctt tactgttgat ttcgtaagcg
    50041 cgttgcacct gaatcatatt gaccagttct tccgccacgt tgacgttaga cgtttcaaca
    50101 tacccttgat acagcagtcc cgcgccgttc aggcccggcg tgctttcgtt cggtgcacca
    50161 gaggattgcg tttcggtgta gaggttttcg ccaatgctct ccagcccggt gtcattcata
    50221 aaggtggtga gattgagctg cccaacctga accggagctg cctggccttg ttgggttacg
    50281 ctgaccacgc catcacgacc gatggtgata cttaacgcat tcgccggaat ggtgatcgct
    50341 ggctgcacct gaaaaccacc agccgtcacc agctgcccgt tctgatccac ctggaaagag
    50401 ccgtcacggg tataggctga tgaaccatct ggcaacatca cctggaaaaa gccctgccct
    50461 ttaatcgcga catctttgct gttgttggtc tgcgacaggt ttccctggct gtgtaagcgt
    50521 tcagtggcga ccgggcgtac ccccgtgccg atttgtaatc cggagggtaa ggtggtttgt
    50581 tcggaagact gtgcccccgg ctggcgaatg gtttgataaa gcagatcttc aaacaccgcg
    50641 cgctgacgct taaaaccgtt agtactgacg tttgccaggt tgttggcaat gacgtccata
    50701 ttggtttgct gggcgtcaag gcccgttttg gcgatccata atgaactgat cataaaatgt
    50761 cctgtagtgg attagtgaca agcagagcag aagcacgcga cagtgtgaat ttcccctcac
    50821 cctaaccctc tccccaaagg ggcgagggaa tagtccgcgc gcttttattg caatgtcatc
    50881 aatcgttcaa ttaactcatc gacagcagtt ggttggcacg gcctgcgtta tcatcgacgc
    50941 tgctgatcac cttcatctgc atttcaaaac gccgcgcgct ggcaatcatg tcgctcattg
    51001 ccgcaacggc attgacgtta ctgccttcca gaacccccga catcacacgc aaggttggat
    51061 ctgcctgcag tatcggccca cgcgtggcct ggctttctgc gcttaaacga aaaatgccgt
    51121 cgtcaccgcg ttgcacttcg ctgcccgtgg ctttcaccag tttcagacgc cccactggcg
    51181 caaccgtatt tgccggatcg cccggattga gcgccgagat tgtgccatcg gcagcaatag
    51241 tgatttccgc cccttccggc acagcaattg gcccagcctc gcctatcacc ggatgcccct
    51301 gaattgtcag ttgcccggtg ggatcaacct gaatgctgcc attacgcgta tacccttcgc
    51361 tgccgtcagc ggactgcacg gccagccagc catcctgctg caatgcaacg tccagcgggc
    51421 gcgaggtgta atccatcttg ccgggcgtca tgtctgcacc cggtgttgac gccgttacca
    51481 gcgtgcgcgt gggcagtgaa agcccttcca ccggcactgc acgcaaggcg tttaactgcg
    51541 cgcgaaaacc gggcgtagag gcattggcca gattactggc ggttaccgcc tgttgattca
    51601 gcgtctggct ggctgctccc atcgcggtat aaattgcgtg atccattgag ctatcccgtc
    51661 agcgattagc gtaagttaac cagcgtgttg aggatctggt cctgggtttt gatggtctgg
    51721 gcgttagact gatagttacg ctgggcaacg atcatattga ccagttcttt actgagatcg
    51781 acgttggacg cttccaacgc accgttggtc agggtgccaa agttgcccgt cccggctgtc
    51841 cccaacagcg ccacgccaga agattgcgtc gcagaccaga cgttgtcgcc ttcggatgcc
    51901 agaccttcgt tgttggcaaa gttcgccagt acaatctgcc ccagcagttg tgtttgttcg
    51961 ttggaatagt tgccgacaac cgtaccgtca tcattgattt gataactcac cagatcgccc
    52021 ggtttgtagc cattctgggt ggttgccaca atattgttag cgccggtatt ttgctgcatg
    52081 gagttgagga agctcaggct aaacgtggcg ggatctgcgc cgttaattgc gccagtggta
    52141 atattctctg ttggatttga ggtcagaacg ccattggcat taaacaccag cttcattgca
    52201 ggctcggctg tacctgttgg atcactgcta tcctgggtgt agacctgcca gttattatcc
    52261 ccggtcttca caaagtagac gctcatgtca tgagcattac cctgactgtc gaaaacagtc
    52321 accgaacctt ttttgttata gctatccgca ttgctggcgc tgaatggcgt aacagtagga
    52381 agcggatcac tggaattcag gttgatctgc atcgatgccg tggtggtagt tttcgctgcc
    52441 atcagggtat tcgggatcga aatattggtc ggattcgccc cttgctgaat agtcggcggc
    52501 gtaccggttg ccgggtaacc cgtcagctgt aaaccttgca tattcaccag gttacggttt
    52561 tcatccagct taaattgtcc gttacggctg tagaacaccg aaccgttgct gtctaccaga
    52621 cggaaaaaac cgttctggct gatagcaacg tccagacctc gcccggtgtt ggtggtcgtg
    52681 ccatcggtaa agtcctgagt gataccggca acttttaccc ccagtcccac tttcgaaccg
    52741 gcaaacatat cggcaaaaga ggccgtgcct gatttaaagc cgtaggtggc ggagttggcg
    52801 atattgttgc caataacatc gaggttggtg gcggcagcgt ttaatccgct aaccgcttga
    52861 gaaaaggcca tgactgactc ctgaaatgtg aaggcttaaa ttatctgccg tacttcgtcg
    52921 agggtggtgg tgccgtaagt gccgagatcc agcgtattac cgctgttgcc gcggatcaca
    52981 ccctgcacca gagcaaactg cagcggctgg gcaaccagtt gtgtaccacc gttactggcg
    53041 ctaatcgcta cattgtaaga accgttcggc gcagtgctgc cgtcagtcaa cgtaccgtcc
    53101 caggtgaaac tgtgaactcc agcggtcagt tcaccaatat caatggtgcg cacaaccgca
    53161 ccatttttat cggtgatggt ggccgtaact ttgtctgccg cctgttgcag ctcaacacca
    53221 aacggcgtgg tcgtggtcac agctccttct tcactgccgg ttcccgcaag aacagtggtg
    53281 ccggggatca tcacgccgtg accgatcagg ttactggcct gtaacgactg gctgttatca
    53341 atctgtccgg aaatagatcc gagtgtggta ttgagttttt caatcccgct gaccgtgctg
    53401 atttgtgcca attgcgacgt cagctcgttg ttttccattg gattggtcgg gtcctggttt
    53461 ttcagctgcg ccaccagcaa agtcagaaaa ctgctttgta aatctgcggc gttgctgccc
    53521 gtgagcgaac tactgctggt ggtactgacg ccggtatttg tcggatcggt ggtggttacc
    53581 gcaatggaca tagctttctc ctttattgac cgagcgtaag ggttttcagc atcatgcttt
    53641 tcaccgtgtt gagcacttca acgttggcct gatagctgcg tgacgccgac atggtgttaa
    53701 ccatctcccc gacgacatca acgttcggca tttttacgta gccctttgca tctgccagcg
    53761 gattacccgg ttcataaacc agtttgtccg gggcctgact ttctataaca tcggcaacct
    53821 ttacgccgcc tgtcgcagca cctggtgcag cgttaacctg gaataccacc tgttttgccc
    53881 gatatggctg tccatcagga ccggtcacgc tatcagcatt tgccagatta ctggccgcca
    53941 cgttcaggcg ctgggactgg gcagttaacg ccgacccggc gatatcaaaa atattcagca
    54001 gtgccatccg ttaatttccg ctctgtaaaa cgttcatcat gcctttgatt tgcccgctca
    54061 acgcgctaag gctcatctgg tattgcaggc tgttatcggc aaactgggtg cgttcgcgat
    54121 ccatatcgac ggtattaccg tcaagcgaag gctggtccgg aatacggtat tgcagttctg
    54181 cggtaggagg cgtcagcgcc tgcgccggga tgtgttgcgt tgaggtcatc gtcagtgcaa
    54241 ccacactggt tgcatcccgt ccacgttgca tgactttttt aagttcactg gcaaaatcga
    54301 tatcgcgcgc ctgataacca ggggtatcgg cattggcgat gtttgctgcc agcacttcct
    54361 gacgctgggc gcgcagattg agcgcctctt gttgaaaacg taaggcggcg tcgagcttat
    54421 cgagcatatc tcctccgcag gtatcaaaat tctgccatca gcttaaatgc ctttacgcgc
    54481 gcgttatcgg cggaataaac gcaaaatggg tcgctattta tgccgttgat ggtcattgcg
    54541 gacaggtaca attcacgttg tagaaatggc tgggggcgaa aatgctgaca ataaaacgta
    54601 gcgtggcgat catcgcgata ctgttcagtc cgttaagtac ggcgagcaat ctcacatcgc
    54661 aattgcacaa cttttttagc gcccaactcg cgggggtaag tgatgaggtt cgtgtttcta
    54721 ttcgtacagc gcccaatcta ctaccgccat gcgagcagcc attgctttcg atgagcaata
    54781 attcccgcct gtggggcaat gtgaatgtgt tggcacgctg cggtaacgac aaacgatatt
    54841 tacaggttaa tgtgcaggcc acaggaaatt atgtggttgc cgcgatgccc attgtgcggg
    54901 gaggaaagct ggaagctggc aatgtcaaac taaaacgcgg acggctggat accctgccac
    54961 cgcgtacggt gctggatatc aatcaacttg ttgatgccat tagcctgcgc gatctatcac
    55021 ccgatcaacc tatccagtta acccagtttc gccaggcatg gcgggtaaaa gcgggacaac
    55081 gcgtcaatgt gatcgccagc ggtgatgggt ttagcgccaa cgcagaaggt caggcgctga
    55141 acaatgcagc cgtcgcacag aatgcgcggg tgcgcatggt atcgggacag gtagtcagcg
    55201 gcgttgttga tgcagatggg aatattctta taaacctgta agctgttaaa gattacccgt
    55261 cccttgccga taaataagca acacatgata aaagcgccct caatgaggaa taaaccatga
    55321 gtattgatcg cacttcgcct ctgaagcctg taagcaccgt tcaaccgcgc gaaaccactg
    55381 acgcgccggt aacgaacagc cgggcggcaa aaacaaccgc ctccaccagc accagtgtga
    55441 cgttaagcga cgcgcaagca aaactgatgc aacccggcag cagtgatatc aatcttgaac
    55501 gtgtcgaagc gttaaaactg gcgattcgta acggtgaact aaaaatggac accggcaaaa
    55561 ttgccgatgc gctgatcaac gaagcgcagc aagacttgca gagtaactga tcgtatgaca
    55621 cgtcttgcag agatcctcga ccagatgtcc gctgtgctta acgatctcaa aacggtaatg
    55681 gatcaagagc agcaacatct ctctatgggg cagatcaacg gcagccagtt gcaatggatt
    55741 acagaacaaa aaagctcact gctggcgacg ctggattacc tcgaacagtt acgcaagaaa
    55801 gaacccaata cagcaaatag cgttgatatt agtcaacgct ggcaggaaat tactgtgaaa
    55861 acgcagcaac tacgccaaat gaatcaacat aacggctggt tactggaagg acagattgag
    55921 cgcaatcaac aggcgctgga aatgttgaaa ccgcatcagg aaccgacgct atatggggcg
    55981 aacggtcaga cctcaacaac ccatcgcggc ggtaaaaaga tttcgatctg aatattatct
    56041 ccggcctgca ctgcaggccg gaatgcattg ttacaccgtc cggcgggcaa attctttaac
    56101 tttgaagccc agtaccgcca gtgcagcgaa gtacgcggca atccccgcca gcacgaccgc
    56161 cattaaacgc agtaagcgcc agggcatggt acccaatgac cactccggca tgatatgtaa
    56221 catacctaaa agcacgccag acatcaccag taccgccatc accagacgca acagaaacgc
    56281 catccagccg ggttgcggag taaagatttt ctgcttacgc aactgccagt aaagcagcga
    56341 agcattcaga cacgccgcca gaccaataga aagtgacagc ccggcatgtt tcaacggacc
    56401 aataaacgcc aggttcatca attgcgttaa aatcagcgta acgatggcaa ttttcactgg
    56461 cgttttaatg tcctggcggg aataaaagcc aggagccaac actttcacta caatcaggcc
    56521 aatcaaaccc accgagtagg caattaacgc ccgctgggtc atcagcgcat caaacgcggt
    56581 aaatttaccg tactggaaca gcgaaacggt taacggacca gaaagaatgc ccaacgcaac
    56641 cgcactcggc agcgccaaca ggaaacaaag acgcaacccc cagtccatca aacggttgta
    56701 ttcatcatga ttgccactgg caaaactttt cgacagcgac ggcagcaaaa tggtgccaag
    56761 cgccacgccc agcacaccgg acggaaactc cattaagcgg tcggcgtaat acatccaaga
    56821 caccgaaccg gaagcaagaa acgaggcaaa aatggtgttg atgattaagg agatctggct
    56881 cacagagacg ccaaggatcg ccggtcccat ctgtttcacc acgcgcattg ttccggcatc
    56941 gtggaagtta atgcgcggca ggaccagcat gccgatcttc ttcaggtgcg gtagctgata
    57001 caccagctgc aggatgccgc cgaccgtaac agcccacgcc agcgccagca ccggtgggtt
    57061 aaagtacggt gcggcaaaca gcgcgaaacc aatcatgctg atgttaagca gtgttggagc
    57121 aaacgccgga atcgagaagc ggttccacgt attcagaatc gctcccacca gcgacgccag
    57181 ggagatcagc aagatatagg gaaaggtaat ctttagtagc tggctggtca gggcaaattt
    57241 gtcagctgtg tcagcgaagc ctggcgcggt caccatgatc acccacggtg cggcgagcat
    57301 gccagcgacc gtcacaaccg ccagcgcgag tgtcagcagg ccagaaacat aagagacaaa
    57361 gacccgcgtg gcgtcttcac cctgcttact tttatattcc gccagaatcg gtacaaatgc
    57421 ctgggaaaat gccccttcgg caaagatacg gcgtaacaag ttaggaagtt taaaagcgac
    57481 gaaaaaggcg tcggttgcca tccctgcgcc aaagattctg gcgacaattg cgtctcgtgc
    57541 gaagccaagc acacgcgaaa acatggtcat cgagctgacg gcggccagcg attttaataa
    57601 attcatcggt gttctaatcc agacccacgg gcaaaacgcc tgcaaggcag gcgctgaaag
    57661 aggcgaatag tctacctgga ttatggtgaa ttgctaccgc cagatgttac agggtttatt
    57721 cactcatcgc atcgcgccag atcttgtcaa cgatacgttg cgccagcacg gcctgttcgc
    57781 cggcggtttg cggaactgtc tgattttgca cacattcaat gaagtgccgc gcacagccga
    57841 caaatccacg ttgttcaaga gtgctctgcc aaccaggaat cggtttatgc actacgccct
    57901 gcccgcgctc ctcacgccat tcgcgcatat ccgtaatgtc gatgagcgca ccgtcagtca
    57961 cggcctgcac ggtttcacgc tgacttccgg cacggcgatg catacaggtg gtgatctgca
    58021 aaggaccggc cgaaaaatgg tgctcggcaa acagcatttc gccagcgtcg ttagtcagta
    58081 gcgtaccgcc atccagagag gctttgccgc ccgacaacca cagcacggta tccaccacat
    58141 gcagataatc atccagcaac gtgaaataaa gatcgtgtgg cccgacacta ttgctacgat
    58201 gtttatccat tcttagcgaa gctgcggtag cgagttgcgt ttttaactca ccgtagagtg
    58261 gtgcgaaacg acggttaaaa ccgaccatca acgtcagttt tttacgcgcc gccagttcca
    58321 ccagccgttc agcatcgcgc agattttctg ccagcggttt atcgacacag acatgtacac
    58381 ccgcattgag taacgtactg accacgtcaa agtggctggc ggtgctggaa tgcacaaaaa
    58441 ccgcatcgca actggcggca aggctggata acgaatcggc ataaggaatg cgccagcttt
    58501 cacaaattgg cagggctttc gcgcgcgtag gcgaccaggc tccttgtaac gtccagtcag
    58561 acgctgccgc cagcaccggt aaccacgctt tttgcgcaat gccacctaat cccactacgc
    58621 cgatacgtaa ttttttcact ttaatctccc agatgggcca gcaacgaatc aagacgctgt
    58681 ttcagttctg ccacttcgat ttccagggct tcgacgcggg cctgtaaatc accatcaacc
    58741 gcgttcgaca tatccgtcac cgccggctga tcttcaacct caccactgaa aagatgcatg
    58801 tagcggcttt cgcgtttacc cggttcgcgg gccagacgca ccacaaaagg accatcttcg
    58861 cgatttgcca gttgttccag cgtcgactcc acttccgcca tatcgctgaa ttcatacatt
    58921 cgcgcggcgc ggctgcgcag ttcacctggc gtctgggcac cacgcaataa caacgtggtg
    58981 attaacgcca cttccgctgc gctcagtttc agatcgccaa attctgaatt acaaaaacgt
    59041 tgctcatatt tggtgacccg attaccaaaa ccgctcactg tgcgtagata atgacgtttg
    59101 accagattat ccagctgttc ctgcacttcg gattcactca gattcatgac cggttcacgg
    59161 ttcgttttct gattacaggc cgtgactaca ccattgactg agagcgggta ttgctccggc
    59221 gtggtcacct gcttttccag caagcaacca atcacgcgag cttcaagggc agtaagttga
    59281 tatttcatga gattctcctt agcggccggg cgtccagtct ggggtagtta atgccgtcag
    59341 tacgtgatcg cgccattgtc catcaatcaa cagatagtct ttcgcatagc cttctttttc
    59401 aaaacccagt cgcgccagta aatcaccgct gcgtttattg tacggcatat aattagccat
    59461 aatgcgatga atatgttggg tgcgctgcat ataacgaatg gctgcggtca gggcttcaaa
    59521 catgagtcct ttgccctgcc atttttgccc aatcgaataa ccgagatagc aggcatgaaa
    59581 agagccacga acaacattgg aaaaattggc aacgccaata atctcttttt cgtccgggtc
    59641 gaataagcca aagtagaaag ctgaaccttg tttatgaaat tcgttaatca tccccagcct
    59701 ggcctgccag cctgatggat aacagtggct ttcgtcgcgc actggctccc agggcttgag
    59761 gaaatggcga ttctctgcgt aataatccgc aagacgccag gcatcacgat catgcaccag
    59821 acgcacgacc agtcggtctg tggttaagcg cacttttggc acgttactgc gatagccaaa
    59881 cattctatac ctactccttc ccgtaacgtc tttaatacgc tgtaagtatt actatacctg
    59941 cgcaaccaga ccctgtgaaa agtccaaaat gccattttct gcattattca catatttgat
    60001 aaaaactctc tatcaaagcg acgttttgat aaaaaaatat tgtccccgaa gtgatgggaa
    60061 aagtcttagc ggcaatgcag aatagaaagt gctcatcttt tatttcccgg gaggggaaat
    60121 gtcccgcgta tcgcaggcga ggaacctggg taaatatttc ctgctcatcg ataatatgct
    60181 ggtcgtgctg gggttctttg ttgtcttccc gctgatctct atccgcttcg ttgatcaaat
    60241 gggctgggcc gccgtcatgg tcggtattgc tctcggtcta cgccagttta ttcagcaagg
    60301 tctgggtatt ttcggcggcg caattgccga ccgctttggt gccaaaccga tgattgttac
    60361 cggtatgctg atgcgcgccg ccggattcgc cacaatgggt atcgcccacg aaccgtggct
    60421 attgtggttt tcatgcctgc tctcgggact cggcggcacg ttgtttgatc cgccgcgttc
    60481 ggcgctggtg gtgaaactaa tccgtccaca acaacgtggt cgttttttct cgctgttgat
    60541 gatgcaggac agtgccggtg cggtcattgg cgcattgttg gggagctggc tgttgcaata
    60601 cgactttcgc ctggtctgcg ccacaggggc cgttttgttt gtgctgtgtg cggcgttcaa
    60661 tgcgtggttg ttaccggcat ggaaactctc caccgtacgc acgcccgttc gcgaaggcat
    60721 gacccgcgtg atgcgtgaca agcgttttgt cacctatgtc ctgacgctgg cgggttacta
    60781 catgctggct gtacaagtga tgctgatgct gccaattatg gtcaacgacg tggctggcgc
    60841 accctctgcc gttaaatgga tgtatgccat tgaagcgtgt ctgtcgttaa cgttgctcta
    60901 ccctatcgcc cgctggagtg aaaagcattt tcgtctggaa caccggttga tggctgggct
    60961 gttgataatg tcattaagca tgatgccggt gggcatggtc agcggcctgc aacaactttt
    61021 caccctgatt tgtctgtttt atatcgggtc gatcattgcc gagcctgcgc gtgaaacctt
    61081 aagtgcttcg ctggcggacg caagagctcg cggcagctat atggggttta gccgtctggg
    61141 tctggcgatt ggcggcgcta ttggttatat cggtggtggc tggctgtttg acctgggcaa
    61201 atcggcgcac cagccagagc ttccgtggat gatgctgggc attattggca tcttcacttt
    61261 ccttgcgctg ggttggcagt ttagccagaa acgcgccgcg cgtcgtttgc ttgaacgcga
    61321 cgcctgattt gatactcccc cctgactcct ccatactgaa agtgaactga cagccaaatg
    61381 gaggagtcac gtgaagctat acatttacga tcactgccct tactgcctca aagcccgcat
    61441 gattttcggc ctgaaaaata tccccgtcga attacatgtt ctgctcaacg acgacgcaga
    61501 aacacccccc cggatggtcg gtcaaaaaca ggttcccatt ctgcaaaaag atgacagccg
    61561 ctatatgcca gaaagcatgg acatcgttca ctatgtcgat aaactcgacg gcaaaccgtt
    61621 actgaccggc aaacgttccc ctgccattga agagtggctg cgcaaggtca atggctacgc
    61681 aaacaaactg ctgttgccgc gttttgccaa atcggcattt gatgagtttt ctactcccgc
    61741 cgcgcgcaaa tatttcgtcg acaagaaaga ggccagcgcg ggtaatttag ccgacctgct
    61801 ggcccactct gacggtctga ttaagaatat cagcgatgat ttgcgtgcgc tggacaaact
    61861 gatcgtcaaa ccgaacgccg tgaatggcga actttcggaa gatgatattc agctatttcc
    61921 gctactgcgt aatctgacgc tggtagccgg aatcaactgg ccaagccgcg ttgctgatta
    61981 ccgcgataat atggcgaaac agacacaaat caatttgtta tcatcaatgg cgatttaatc
    62041 ctgacccgcc gcggcagcct gtattcgcgg gttgccgccg ttttcagttt tttctgctgg
    62101 cgagtttatc gctggtgaca catgctgcgt tgtgtcagga tattgacgca gatttcatgt
    62161 cgaggactac catgaacaag tttttatttg ccgctgcatt gatcgtcagt ggcctgctcg
    62221 ttggctgtaa tcaactcacc caatacacca tcaccgaaca agaaattaac cagtcgcttg
    62281 cgaaacataa taatttctca aaagatatcg gtttacccgg cgtggctgac gcccatattg
    62341 ttctgacaaa cttgaccagc caaattggtc gcgaagagcc gaataaggtt accctaaccg
    62401 gagacgccaa tctggacatg aactccctgt tcggtagtca gaaagcgacc atgaaactga
    62461 agctgaaagc gctgcccgtg tttgataaag aaaaaggtgc gatcttcctg aaagagatgg
    62521 aagtggtcga tgcgacggta caaccggaaa aaatgcaaac ggtgatgcaa acgttgcttc
    62581 cctatttgaa ccaggcatta cgcaattact ttaaccagca acctgcttac gtcctgcgcg
    62641 aagatggcag tcagggcgaa gcaatggcga aaaaactggc gaaaggcatt gaagtgaagc
    62701 caggcgaaat tgtcattccg tttacggatt aatgacgagg gcgcattcgc gccctttatt
    62761 tttcgtgcaa aggaaaacgt ttccgcttat cctttgtgtc cggcaaaaac atcccttcag
    62821 ccggagcata gagattaatg actgcaccat cccaggtatt aaagatccgc cgcccagacg
    62881 actggcacct tcacctccgc gatggcgaca tgttaaaaac tgtcgtgccg tataccagcg
    62941 aaatttatgg acgggctatt gtaatgccca atctggctcc gcccgtgacc actgttgagg
    63001 ctgccgtggc gtatcgccag cgtattcttg atgccgtacc tgccgggcac aatttcaccc
    63061 cattgatgac ctgttattta acagattcgc tggatcctaa tgagctggag cgcggattta
    63121 acgaaggcgt gttcaccgct gcaaaacttt atccggcaaa cgcaaccact aactccagcc
    63181 acggcgtgac gtcaattgac gcaatcatgc cggtacttga gcgcatggaa aaaatcggta
    63241 tgccgctact ggtgcatggt gaagtgacac atgcagatat cgacattttt gatcgtgaag
    63301 cgcgctttat agaaagcgtg atggaacctc tgcgccagcg cctgactgcg ctgaaagtcg
    63361 tttttgagca catcaccacc aaagatgctg ccgactatgt ccgtgacgga aatgaacggc
    63421 tggctgccac catcactccg cagcatctga tgtttaaccg caaccatatg ctggttggtg
    63481 gcgtgcgtcc gcacctgtat tgtttaccca tcctcaaacg caatattcac caacaggcat
    63541 tgcgtgaact ggtcgccagc ggttttaatc gagtattcct cggtacggat tctgcgccac
    63601 atgcacgtca tcgcaaagag agcagttgcg gctgcgcggg ctgcttcaac gccccaaccg
    63661 cgctgggcag ttacgctacc gtctttgaag agatgaatgc tctgcagcac tttgaagcat
    63721 tctgttctgt aaacggcccg cagttctatg gcttgccggt caacgacaca ttcatcgaac
    63781 tggtacgtga agagcaacag gttgctgaaa gcatcgcact gactgatgac accctggtgc
    63841 cattcctcgc cggggaaacg gtacgctggt ccgttaaaca ataaaaatcg tagcttcctg
    63901 ttgtcattag gttattttac ctgtataaat aaccagtata ttcaacaggg ggctattatg
    63961 cgaattgaag tcaccatagc gaaaacttct ccattgccag ctggggctat tgacgccctg
    64021 gctggcgaac tttcccgccg tattcagtat gcgtttcctg ataatgaagg ccacgtatcg
    64081 gtacgttatg ccgcagcgaa taatttatcg gttattggcg caacaaaaga agataaacag
    64141 cgcattagcg aaattctcca ggaaacgtgg gaaagcgccg atgactggtt tgtcagcgaa
    64201 taatatgcag tgatttttgt ttttgccggg tcgccccggc ttttttattt tacttacaaa
    64261 ttttgagtac aaaatttcta cacttcctca aaataattgc acaatatgct aaaaaaatat
    64321 gaatgacatg ctgttttatt gttcatataa atcttaaaag cagaaattta tctgttcccc
    64381 cctttaaaaa ttcggtgaat acccttactt attggtatac tgaagttgct tcagataaaa
    64441 acgcattgaa cctcgaataa cgttgtctag taacacgaat tagggggcca tgatggaaaa
    64501 aaataatgaa gtcattcaga ctcatccgct cgtagggtgg gacatcagca ccgttgatag
    64561 ctatgatgcg ctgatgttgc gtttgcacta ccagacccca aataagtccg agctggaagg
    64621 gactgaagtt ggtcagacgc tctggttaac cactgatgtt gccagacaat ttatttcgat
    64681 attagaagca ggaatcgcca aaattgaatc cggtgatttc ccggtaaacg agtatcggcg
    64741 tcattaacat gacatgacca tccacccaaa aggcacctca ccggtgcctt taccatttct
    64801 gggttgtata tctgcattca gttaattact ctgcttaaac catgagagca gagagaaaaa
    64861 aatgaaatac gatctcatca ttattggcag cggttccgta ggcgctgccg ccgggtatta
    64921 tgcaacccgc gccggtttaa acgtgctaat gaccgacgcc catatgccac cgcatcaaca
    64981 cggcagccac catggcgata cgcgattaat tcgccatgct tatggtgaag gcgaaaagta
    65041 tgtcccgctg gtcctccgcg cgcaaacgct gtgggatgaa ctttcccgcc acaacgaaga
    65101 tgatcccatt tttgtacgct ctggtgtcat taacctcggc ccggctgact ccgcatttct
    65161 cgccaacgtc gcccacagcg ccgaacagtg gcaactcaac gttgaaaagc tcgatgcgca
    65221 agggattatg gcccgctggc cagaaatacg cgtcccggac aactacatcg gcttatttga
    65281 gactgattcc ggttttttgc gcagcgaact ggcgattaaa acctggatcc aactggcgaa
    65341 ggaagcgggc tgtgcgcaac tgttcaactg cccggtcacc gtaattcgtc atgacgatga
    65401 tggcgtaact attgaaacgg ttgacggtga gtatcaggcg aaaaaagcga ttgtctgcgc
    65461 gggaacatgg gtaaaagacc tgctcccgga gctgcctgtc cagcccgtac gtaaagtatt
    65521 tgcctggtat caggccgatg gccgctatag cgtgaagaat aaattcccgg cgtttaccgg
    65581 tgaactgccc aatggcgatc aatattatgg ttttccggca gaaaacgacg cgttgaagat
    65641 tggcaaacat aacggaggcc aggttatcca ttcagcggat gaacgtgttc cgtttgcgga
    65701 agtggtcagc gatggttcgg aagccttccc gttcttgcgc aatgtattgc cgggtatcgg
    65761 ttgctgcctg tacggcgctg cctgcaccta tgataattcg cctgacgaag attttattat
    65821 cgatacccta cccggccacg ataatacact gctcattacc ggcctgagtg ggcacggttt
    65881 taaatttgcg tcagttttag gggaaatagc tgccgatttt gcgcaagaca aaaaaagcga
    65941 ttttgatttg acgccattca ggctttcccg cttccaataa tcatcattac ggcctccaga
    66001 atgaggccgt ttttacagga gtatttaatg cgtcggctgt tgcactatct catcaataat
    66061 attcgcgagc atctgatgct ttatcttttt ctctggggat tgttggcaat tatggatctg
    66121 atctatgtgt tttattttta gttttccgca ctgacataaa ctgacaaata tttaatctcc
    66181 ctttcatttg gcgtgaaaaa ttgaatgaga ttattcctgc ttctacagca ctcaccagac
    66241 atctctg
//
LOCUS       DAZFWL020000009        65707 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFWL020000009 DAZFWL020000000
VERSION     DAZFWL020000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 65707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.009"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(95..763)
                     /gene="ydcL"
                     /locus_tag="ACHKNP_RS03320"
                     /old_locus_tag="pgaptmp_000664"
     CDS             complement(95..763)
                     /gene="ydcL"
                     /locus_tag="ACHKNP_RS03320"
                     /old_locus_tag="pgaptmp_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HGU4702891.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(871..1230)
                     /locus_tag="ACHKNP_RS03325"
                     /old_locus_tag="pgaptmp_000665"
                     /pseudo
     CDS             complement(871..1230)
                     /locus_tag="ACHKNP_RS03325"
                     /old_locus_tag="pgaptmp_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(1403..1996)
                     /gene="tehB"
                     /locus_tag="ACHKNP_RS03330"
                     /old_locus_tag="pgaptmp_000666"
     CDS             complement(1403..1996)
                     /gene="tehB"
                     /locus_tag="ACHKNP_RS03330"
                     /old_locus_tag="pgaptmp_000666"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HGU4702892.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(1993..2985)
                     /gene="tehA"
                     /locus_tag="ACHKNP_RS03335"
                     /old_locus_tag="pgaptmp_000667"
     CDS             complement(1993..2985)
                     /gene="tehA"
                     /locus_tag="ACHKNP_RS03335"
                     /old_locus_tag="pgaptmp_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HGU4702893.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3109..4089
                     /gene="ydcK"
                     /locus_tag="ACHKNP_RS03340"
                     /old_locus_tag="pgaptmp_000668"
     CDS             3109..4089
                     /gene="ydcK"
                     /locus_tag="ACHKNP_RS03340"
                     /old_locus_tag="pgaptmp_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HGU4702894.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDGVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4084..4620)
                     /gene="rimL"
                     /locus_tag="ACHKNP_RS03345"
                     /old_locus_tag="pgaptmp_000669"
     CDS             complement(4084..4620)
                     /gene="rimL"
                     /locus_tag="ACHKNP_RS03345"
                     /old_locus_tag="pgaptmp_000669"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HGU4702895.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4683..4907)
                     /gene="ydcH"
                     /locus_tag="ACHKNP_RS03350"
                     /old_locus_tag="pgaptmp_000670"
     CDS             complement(4683..4907)
                     /gene="ydcH"
                     /locus_tag="ACHKNP_RS03350"
                     /old_locus_tag="pgaptmp_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HGU4702896.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5047..6702)
                     /gene="opgD"
                     /locus_tag="ACHKNP_RS03355"
                     /old_locus_tag="pgaptmp_000671"
                     /gene_synonym="mdoD"
     CDS             complement(5047..6702)
                     /gene="opgD"
                     /locus_tag="ACHKNP_RS03355"
                     /old_locus_tag="pgaptmp_000671"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HGU4702897.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(6927..8270)
                     /gene="ydcJ"
                     /locus_tag="ACHKNP_RS03360"
                     /old_locus_tag="pgaptmp_000672"
     CDS             complement(6927..8270)
                     /gene="ydcJ"
                     /locus_tag="ACHKNP_RS03360"
                     /old_locus_tag="pgaptmp_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4702898.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8487..9410
                     /gene="ydcI"
                     /locus_tag="ACHKNP_RS03365"
                     /old_locus_tag="pgaptmp_000673"
     CDS             8487..9410
                     /gene="ydcI"
                     /locus_tag="ACHKNP_RS03365"
                     /old_locus_tag="pgaptmp_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4702899.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9448..11088)
                     /gene="trg"
                     /locus_tag="ACHKNP_RS03370"
                     /old_locus_tag="pgaptmp_000674"
     CDS             complement(9448..11088)
                     /gene="trg"
                     /locus_tag="ACHKNP_RS03370"
                     /old_locus_tag="pgaptmp_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HGU4702900.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            11487..11669
                     /locus_tag="ACHKNP_RS03375"
                     /old_locus_tag="pgaptmp_000675"
     CDS             11487..11669
                     /locus_tag="ACHKNP_RS03375"
                     /old_locus_tag="pgaptmp_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HGU4702901.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSRLGQARGFSPRI"
     gene            complement(11708..11881)
                     /locus_tag="ACHKNP_RS03380"
                     /old_locus_tag="pgaptmp_000676"
     CDS             complement(11708..11881)
                     /locus_tag="ACHKNP_RS03380"
                     /old_locus_tag="pgaptmp_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4702902.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(12126..12656)
                     /gene="cybB"
                     /locus_tag="ACHKNP_RS03385"
                     /old_locus_tag="pgaptmp_000677"
     CDS             complement(12126..12656)
                     /gene="cybB"
                     /locus_tag="ACHKNP_RS03385"
                     /old_locus_tag="pgaptmp_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HGU4702903.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            12845..13846
                     /gene="gap"
                     /locus_tag="ACHKNP_RS03390"
                     /old_locus_tag="pgaptmp_000678"
     CDS             12845..13846
                     /gene="gap"
                     /locus_tag="ACHKNP_RS03390"
                     /old_locus_tag="pgaptmp_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU4702904.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFLWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(13888..15327)
                     /gene="aldA"
                     /locus_tag="ACHKNP_RS03395"
                     /old_locus_tag="pgaptmp_000679"
     CDS             complement(13888..15327)
                     /gene="aldA"
                     /locus_tag="ACHKNP_RS03395"
                     /old_locus_tag="pgaptmp_000679"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGU4702905.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPAAEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(15524..16324)
                     /gene="ydcF"
                     /locus_tag="ACHKNP_RS03400"
                     /old_locus_tag="pgaptmp_000680"
     CDS             complement(15524..16324)
                     /gene="ydcF"
                     /locus_tag="ACHKNP_RS03400"
                     /old_locus_tag="pgaptmp_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HGU4702906.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(16596..20498)
                     /gene="hrpA"
                     /locus_tag="ACHKNP_RS03405"
                     /old_locus_tag="pgaptmp_000681"
     CDS             complement(16596..20498)
                     /gene="hrpA"
                     /locus_tag="ACHKNP_RS03405"
                     /old_locus_tag="pgaptmp_000681"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HGU4702907.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRIDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            20699..21304
                     /gene="azoR"
                     /locus_tag="ACHKNP_RS03410"
                     /old_locus_tag="pgaptmp_000682"
     CDS             20699..21304
                     /gene="azoR"
                     /locus_tag="ACHKNP_RS03410"
                     /old_locus_tag="pgaptmp_000682"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HGU4702908.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAVPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(21355..22671)
                     /gene="ynbD"
                     /locus_tag="ACHKNP_RS03415"
                     /old_locus_tag="pgaptmp_000683"
     CDS             complement(21355..22671)
                     /gene="ynbD"
                     /locus_tag="ACHKNP_RS03415"
                     /old_locus_tag="pgaptmp_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HGU4702909.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(22661..24418)
                     /gene="ynbC"
                     /locus_tag="ACHKNP_RS03420"
                     /old_locus_tag="pgaptmp_000684"
     CDS             complement(22661..24418)
                     /gene="ynbC"
                     /locus_tag="ACHKNP_RS03420"
                     /old_locus_tag="pgaptmp_000684"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HGU4702910.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(24434..25330)
                     /gene="ynbB"
                     /locus_tag="ACHKNP_RS03425"
                     /old_locus_tag="pgaptmp_000685"
     CDS             complement(24434..25330)
                     /gene="ynbB"
                     /locus_tag="ACHKNP_RS03425"
                     /old_locus_tag="pgaptmp_000685"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4702911.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(25330..25935)
                     /gene="ynbA"
                     /locus_tag="ACHKNP_RS03430"
                     /old_locus_tag="pgaptmp_000686"
     CDS             complement(25330..25935)
                     /gene="ynbA"
                     /locus_tag="ACHKNP_RS03430"
                     /old_locus_tag="pgaptmp_000686"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4702912.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(26106..28412)
                     /gene="ydbD"
                     /locus_tag="ACHKNP_RS03435"
                     /old_locus_tag="pgaptmp_000687"
     CDS             complement(26106..28412)
                     /gene="ydbD"
                     /locus_tag="ACHKNP_RS03435"
                     /old_locus_tag="pgaptmp_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HGU4702913.1"
                     /translation="MFKSRICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNNTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(28476..29336)
                     /gene="pdxI"
                     /locus_tag="ACHKNP_RS03440"
                     /old_locus_tag="pgaptmp_000688"
     CDS             complement(28476..29336)
                     /gene="pdxI"
                     /locus_tag="ACHKNP_RS03440"
                     /old_locus_tag="pgaptmp_000688"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HGU4702914.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(29567..30157)
                     /gene="paaY"
                     /locus_tag="ACHKNP_RS03445"
                     /old_locus_tag="pgaptmp_000689"
     CDS             complement(29567..30157)
                     /gene="paaY"
                     /locus_tag="ACHKNP_RS03445"
                     /old_locus_tag="pgaptmp_000689"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HGU4702915.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(30139..31089)
                     /gene="paaX"
                     /locus_tag="ACHKNP_RS03450"
                     /old_locus_tag="pgaptmp_000690"
     CDS             complement(30139..31089)
                     /gene="paaX"
                     /locus_tag="ACHKNP_RS03450"
                     /old_locus_tag="pgaptmp_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HGU4702916.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(31190..32503)
                     /gene="paaK"
                     /locus_tag="ACHKNP_RS03455"
                     /old_locus_tag="pgaptmp_000691"
     CDS             complement(31190..32503)
                     /gene="paaK"
                     /locus_tag="ACHKNP_RS03455"
                     /old_locus_tag="pgaptmp_000691"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HGU4702917.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGGAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(32530..33735)
                     /gene="paaJ"
                     /locus_tag="ACHKNP_RS03460"
                     /old_locus_tag="pgaptmp_000692"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(32530..33735)
                     /gene="paaJ"
                     /locus_tag="ACHKNP_RS03460"
                     /old_locus_tag="pgaptmp_000692"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HGU4702918.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPHSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(33735..34157)
                     /gene="paaI"
                     /locus_tag="ACHKNP_RS03465"
                     /old_locus_tag="pgaptmp_000693"
     CDS             complement(33735..34157)
                     /gene="paaI"
                     /locus_tag="ACHKNP_RS03465"
                     /old_locus_tag="pgaptmp_000693"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HGU4702919.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(34147..35574)
                     /gene="paaH"
                     /locus_tag="ACHKNP_RS03470"
                     /old_locus_tag="pgaptmp_000694"
     CDS             complement(34147..35574)
                     /gene="paaH"
                     /locus_tag="ACHKNP_RS03470"
                     /old_locus_tag="pgaptmp_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HGU4702920.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(35576..36364)
                     /gene="paaG"
                     /locus_tag="ACHKNP_RS03475"
                     /old_locus_tag="pgaptmp_000695"
     CDS             complement(35576..36364)
                     /gene="paaG"
                     /locus_tag="ACHKNP_RS03475"
                     /old_locus_tag="pgaptmp_000695"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HGU4702921.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(36364..37131)
                     /gene="paaF"
                     /locus_tag="ACHKNP_RS03480"
                     /old_locus_tag="pgaptmp_000696"
     CDS             complement(36364..37131)
                     /gene="paaF"
                     /locus_tag="ACHKNP_RS03480"
                     /old_locus_tag="pgaptmp_000696"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HGU4702922.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(37128..38198)
                     /gene="paaE"
                     /locus_tag="ACHKNP_RS03485"
                     /old_locus_tag="pgaptmp_000697"
     CDS             complement(37128..38198)
                     /gene="paaE"
                     /locus_tag="ACHKNP_RS03485"
                     /old_locus_tag="pgaptmp_000697"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HGU4702923.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGERLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(38206..38703)
                     /gene="paaD"
                     /locus_tag="ACHKNP_RS03490"
                     /old_locus_tag="pgaptmp_000698"
     CDS             complement(38206..38703)
                     /gene="paaD"
                     /locus_tag="ACHKNP_RS03490"
                     /old_locus_tag="pgaptmp_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HGU4702924.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(38718..39464)
                     /gene="paaC"
                     /locus_tag="ACHKNP_RS03495"
                     /old_locus_tag="pgaptmp_000699"
     CDS             complement(38718..39464)
                     /gene="paaC"
                     /locus_tag="ACHKNP_RS03495"
                     /old_locus_tag="pgaptmp_000699"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HGU4702925.1"
                     /translation="MNQLTAYTLRLGDNYLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGIDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(39473..39760)
                     /gene="paaB"
                     /locus_tag="ACHKNP_RS03500"
                     /old_locus_tag="pgaptmp_000700"
     CDS             complement(39473..39760)
                     /gene="paaB"
                     /locus_tag="ACHKNP_RS03500"
                     /old_locus_tag="pgaptmp_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HGU4702926.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(39772..40701)
                     /gene="paaA"
                     /locus_tag="ACHKNP_RS03505"
                     /old_locus_tag="pgaptmp_000701"
     CDS             complement(39772..40701)
                     /gene="paaA"
                     /locus_tag="ACHKNP_RS03505"
                     /old_locus_tag="pgaptmp_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HGU4702927.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            40986..43031
                     /gene="paaZ"
                     /locus_tag="ACHKNP_RS03510"
                     /old_locus_tag="pgaptmp_000702"
     CDS             40986..43031
                     /gene="paaZ"
                     /locus_tag="ACHKNP_RS03510"
                     /old_locus_tag="pgaptmp_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HGU4702928.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            43279..45552
                     /gene="tynA"
                     /locus_tag="ACHKNP_RS03515"
                     /old_locus_tag="pgaptmp_000703"
     CDS             43279..45552
                     /gene="tynA"
                     /locus_tag="ACHKNP_RS03515"
                     /old_locus_tag="pgaptmp_000703"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HGU4702929.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(45610..47109)
                     /gene="feaB"
                     /locus_tag="ACHKNP_RS03520"
                     /old_locus_tag="pgaptmp_000704"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(45610..47109)
                     /gene="feaB"
                     /locus_tag="ACHKNP_RS03520"
                     /old_locus_tag="pgaptmp_000704"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HGU4702930.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            47345..48250
                     /gene="feaR"
                     /locus_tag="ACHKNP_RS03525"
                     /old_locus_tag="pgaptmp_000705"
     CDS             47345..48250
                     /gene="feaR"
                     /locus_tag="ACHKNP_RS03525"
                     /old_locus_tag="pgaptmp_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HGU4702931.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGHLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(48422..48751)
                     /gene="ydbL"
                     /locus_tag="ACHKNP_RS03530"
                     /old_locus_tag="pgaptmp_000706"
     CDS             complement(48422..48751)
                     /gene="ydbL"
                     /locus_tag="ACHKNP_RS03530"
                     /old_locus_tag="pgaptmp_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HGU4702932.1"
                     /translation="MMKKTLLFCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(48756..48941)
                     /gene="ynbE"
                     /locus_tag="ACHKNP_RS03535"
                     /old_locus_tag="pgaptmp_000707"
     CDS             complement(48756..48941)
                     /gene="ynbE"
                     /locus_tag="ACHKNP_RS03535"
                     /old_locus_tag="pgaptmp_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HGU4702933.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(48938..51577)
                     /gene="ydbH"
                     /locus_tag="ACHKNP_RS03540"
                     /old_locus_tag="pgaptmp_000708"
     CDS             complement(48938..51577)
                     /gene="ydbH"
                     /locus_tag="ACHKNP_RS03540"
                     /old_locus_tag="pgaptmp_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HGU4702934.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNSEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            51785..52774
                     /gene="ldhA"
                     /locus_tag="ACHKNP_RS03545"
                     /old_locus_tag="pgaptmp_000709"
     CDS             51785..52774
                     /gene="ldhA"
                     /locus_tag="ACHKNP_RS03545"
                     /old_locus_tag="pgaptmp_000709"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU4702935.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            52885..53307
                     /gene="hslJ"
                     /locus_tag="ACHKNP_RS03550"
                     /old_locus_tag="pgaptmp_000710"
     CDS             52885..53307
                     /gene="hslJ"
                     /locus_tag="ACHKNP_RS03550"
                     /old_locus_tag="pgaptmp_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HGU4702936.1"
                     /translation="MKKLAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(53304..53570)
                     /gene="ydbJ"
                     /locus_tag="ACHKNP_RS03555"
                     /old_locus_tag="pgaptmp_000711"
     CDS             complement(53304..53570)
                     /gene="ydbJ"
                     /locus_tag="ACHKNP_RS03555"
                     /old_locus_tag="pgaptmp_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HGU4702937.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            53844..57368
                     /gene="nifJ"
                     /locus_tag="ACHKNP_RS03560"
                     /old_locus_tag="pgaptmp_000712"
     CDS             53844..57368
                     /gene="nifJ"
                     /locus_tag="ACHKNP_RS03560"
                     /old_locus_tag="pgaptmp_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HGU4702938.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     TWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            57735..58868
                     /gene="ompN"
                     /locus_tag="ACHKNP_RS03565"
                     /old_locus_tag="pgaptmp_000713"
     CDS             57735..58868
                     /gene="ompN"
                     /locus_tag="ACHKNP_RS03565"
                     /old_locus_tag="pgaptmp_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HGU4702939.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            59009..59443
                     /gene="uspF"
                     /locus_tag="ACHKNP_RS03570"
                     /old_locus_tag="pgaptmp_000714"
     CDS             59009..59443
                     /gene="uspF"
                     /locus_tag="ACHKNP_RS03570"
                     /old_locus_tag="pgaptmp_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HGU4702940.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            59619..60554
                     /gene="ttcA"
                     /locus_tag="ACHKNP_RS03575"
                     /old_locus_tag="pgaptmp_000715"
     CDS             59619..60554
                     /gene="ttcA"
                     /locus_tag="ACHKNP_RS03575"
                     /old_locus_tag="pgaptmp_000715"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HGU4702941.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(60683..62056)
                     /gene="dbpA"
                     /locus_tag="ACHKNP_RS03580"
                     /old_locus_tag="pgaptmp_000716"
     CDS             complement(60683..62056)
                     /gene="dbpA"
                     /locus_tag="ACHKNP_RS03580"
                     /old_locus_tag="pgaptmp_000716"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HGU4702942.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(62086..62259)
                     /gene="ynaL"
                     /locus_tag="ACHKNP_RS03585"
                     /old_locus_tag="pgaptmp_000717"
     CDS             complement(62086..62259)
                     /gene="ynaL"
                     /locus_tag="ACHKNP_RS03585"
                     /old_locus_tag="pgaptmp_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HGU4702943.1"
                     /translation="MTTLIYLQIPVPEPIPSDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(62534..63517)
                     /gene="zntB"
                     /locus_tag="ACHKNP_RS03590"
                     /old_locus_tag="pgaptmp_000718"
     CDS             complement(62534..63517)
                     /gene="zntB"
                     /locus_tag="ACHKNP_RS03590"
                     /old_locus_tag="pgaptmp_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HGU4702944.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(63531..63578)
                     /gene="ydaN"
                     /locus_tag="ACHKNP_RS03595"
                     /old_locus_tag="pgaptmp_000719"
     CDS             complement(63531..63578)
                     /gene="ydaN"
                     /locus_tag="ACHKNP_RS03595"
                     /old_locus_tag="pgaptmp_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HGU4702945.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            63772..65004
                     /gene="dgcM"
                     /locus_tag="ACHKNP_RS03600"
                     /old_locus_tag="pgaptmp_000720"
     CDS             63772..65004
                     /gene="dgcM"
                     /locus_tag="ACHKNP_RS03600"
                     /old_locus_tag="pgaptmp_000720"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HGU4702946.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(65025..65588)
                     /gene="smrA"
                     /locus_tag="ACHKNP_RS03605"
                     /old_locus_tag="pgaptmp_000721"
     CDS             complement(65025..65588)
                     /gene="smrA"
                     /locus_tag="ACHKNP_RS03605"
                     /old_locus_tag="pgaptmp_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HGU4702947.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
BASE COUNT        16709 a        17157 c        15629 g        16212 t
ORIGIN      
        1 aggtggaaaa ccgcacctta tatccccgcc ctgaaggatg gggttttacg gcacaccgga
       61 taaaaagcgc catctaacga tggcgcgtct ggaactactt tttgttaacg tcaaacatgg
      121 tggcatcggt cgccatgtca tcaataactt gtttaatatt ttcgaaagcc attggcgtac
      181 tttcgttatt caggtcttta ccttcgccct gacgaacgac tttgataacc ggtttattag
      241 tcgctgcatc aatcagctca ccttcaaaat agaggcgagt atccatggta cggtggcctg
      301 tagccatttg cgtccccgca accactaatg caacaggaac cacttcatag aattgcagcc
      361 cttctttgct ggtatctaca ccggtaatgg ccccacggaa aatcagacta cgcggcccag
      421 cggtggtaac cagtggttta cgctgcgcta tcgcttcttt catttcggtg ttggtatagt
      481 tcaaaatttt atccagaact ttctgcccta cctgggtaga aggttttggc accggataat
      541 aagtgattgg gttccagacg atgctgtcat atttgctttg atcaaaactc gggtctaccc
      601 aacgtaaaac aggtttacct gttgctgagg ttgtttcttt taaatcagag taattgttta
      661 aaaaaccaga atatttatct ggctgggtga ttttagaggc acaaccagac agagccaaca
      721 agccgcataa agctgcaact ttcgcaaatg atgtggtacg catgaatagt ttcctgagat
      781 gattatatta ctttttaagt tatagcaaaa agcgccgctt cgtgttgtgg caaaaaaagt
      841 gttggtgaaa ataaattttt cagccccttt tcagacgaca tagtcaatgc cgatgaaggt
      901 cgcatcagcg tttgcagtac cagctacgga ctgcccgaga gcaatataac gagcctggaa
      961 tggcagtata ttcgtgccat caatcaagtc ggttttagca ctaaaagtgg ccccatcaag
     1021 tattaatacc tcacccgtac ggtccaaaat ttgaataccg acattttggg cagagcctgc
     1081 cgctgaactc tgcagcgcta acgtatcgtc attactggtg acagtagtac ccaggaatgc
     1141 aacagctgca ttactggaaa cattggtatc acaatcattc agcttgatat tgaagccaac
     1201 ggcggagctg aggtcacccg ctttagccaa aaaaggggct aagtcaatca tcagtagtta
     1261 aatttcagta agttaagctg ctaaaaaact tcgactactg tgattaattt cacatccctt
     1321 cgataaaaaa tatgcttacc gaaaagaaga acagtaaatc atctcagcgt ggggaatttt
     1381 cacgcgcctt attcttagcg ggtcattttt tacgtgccag catcgtggcg aaacgcagtt
     1441 taatacgatt accgttggcg tcggtgcggt gcagctcgcc gacgtcttca ttgtatttca
     1501 ccctctccca gccttcgtaa tatcgacgta attctccctc tttgaaggca aacgggaagc
     1561 cgacggtaca tggataatca gcggtatcca tcgccgccac aatcaggttg taaccaccag
     1621 gtttagtgca acgttgcata ttggcaatca gccctgggat ggttttagcc tcaaggaaca
     1681 tcagtaccac agtcgaaaga ataaaatcgt actgtctatc aaatgtgagg ttattcagat
     1741 caacgactcg ggtgtgtaaa ttatccagat tttcaatgga tttaatgcgc tctacattgg
     1801 cgatactcat ggcattttta tcccatgcgt caacatcata accattggct gccaggtaaa
     1861 gactgttacg accattgcca cagcccagat ccagcgtttt acccggttta accactttca
     1921 ccgcttccag tacttcagag tgtgtgcggg ttaattcata tttatcagta aaatagtttt
     1981 cgtcacgaat gatcattctt tgtcctctgc tttcattaaa acggcgcgct cggttctgac
     2041 taacaatttt ccctgcatca gaagcgcaaa agtacggatg agcagtattg caataataaa
     2101 attggtaaag ataaacagcg gtaccgccag cgtatggaaa aatccatttt cgctgccact
     2161 ccccagatgc aaaccggtgg ttgccagtgc agatacgccg aacgagaaac tccagaatga
     2221 agcattaaat ggctgggaga gataccatgg catcagacgt agcataaaca gcagttgcag
     2281 cagtccataa ccaaaaagca ttttcgccag cgtatcaccc tcgccgccgt tgacgctcaa
     2341 ccaggcacta caggccacca gcgcaggagc gagctgaatg ccgagtgatg tccgcagtgc
     2401 cgtgggtaat tctcccgaac tgcgcagacg ctgcaagatc acaggttcaa ggcttagcca
     2461 tgagaaaacg cctgcgccta aaaacaccag accggcgtcg gtgtagccca acgcaccaca
     2521 ggccattgcg ctgataaagt tgttggcaac tgtcggcaga tacagtccag gcgtggtagc
     2581 ttcttcaggg tgagatccgc gccataatcc cgcagtttgc caggcggcat aagccaactg
     2641 aaccacgaca ccaaaactga acaggcacac cgccagtggg cgaaaccacg gaacaaaacc
     2701 aatcgccacc agcatcgtcg ttgccggaaa caaactcaca aaactgctca gcactggatg
     2761 gcgaacttcc gccagcacgc tatgcggaaa gcgtatgagt cgggtaataa atgcgctagt
     2821 taataatccc cagatgatca tcgccagaat caccagccca tcccctaacc agtggctgac
     2881 ctgccaaacc tggctggcat agcgccaggc aaatcccatc cctatcgtcc ccaacacaat
     2941 accaaagtag cctgccggca aattgagcac tttatcgctc tgcatcttct tactcatttt
     3001 gtttaattta taaagtatat ttgaaatgca ttttatggaa ttttctcgag catagccaga
     3061 gccgcagaat ttgctacggt tatagtaagt cacctgtggg aaaacgctat gcgcaaatat
     3121 cgtctaagtg aagaacagcg agcctttagt tatcaggaag atggcactaa aaaaaatgtg
     3181 ttattacggc agattatcgc cattagcgat tttaacgatg tgatagcagg aaccgctggc
     3241 ggctggatcg atcgcgaaac ggtgttagca caggaaggaa attgctggat ttacgaccag
     3301 aatgctattg cgttcggcgg aacggtgatt tccggtaata cgcgcatcac cggcaccagc
     3361 gtactgtggg gagaggttta cgcaacggat aatgtctgga tcgataatag tgaaatcagc
     3421 cagggtgcat atataagtga cggtgtcacc attcatgact cactggtttg cgggcaatgt
     3481 agaatatttg gtcacgcctt aattaatcaa cattctatga tcgtcgcagc acaaggctta
     3541 acgcctgacc atcaactcct gctacaaatt tatgatcgcg caagggttag cgcctcgcgt
     3601 attgtccacc aggcacaaat ttatggcgat gccgtcatcc ggtatgcttt tattgaacat
     3661 cgtgccgaag tttttgattt tgccagtatt gaaggtaatg aagagaacaa cgtctggcta
     3721 tgcgattgcg ctaaagtcta cggtcacgcg caggtgaaag ctggcataga agaagatgcc
     3781 attccaacga ttcattacag ttcgcaggtg gcggaatatg ccattgtgga aggtaattgt
     3841 gtgttaaaac atcatgtctt gattggcgga aatgccgtgg tacgtggtgg accgattctg
     3901 ctcgacgagc acgttgtaat acaaggtgag agccgtatta caggtgcggt gataattgaa
     3961 aaccatgtgg agttaaccga ccacgccgtc gttgaagcct ttgatggcga cacggttcat
     4021 gtccgtggtc caaaagtcat taacggcgag gagcgcatta cgcgaacgcc gctggcaggg
     4081 ttattatgaa tcgataatac gcgcgtataa gttcacatca tcataggcat cattcaggaa
     4141 ctcagcctgt ttcaggcaac cttcaaggat aaaaccattg cgcaaagcga cctggttgct
     4201 ttgcggattg tccacccgac atttgatcac gaagcgtcta agttcaccag actgggcgta
     4261 atgatgaatc aatgcctgca gcgcctgaga aatgatcccc tgcccctgat gagattcgtc
     4321 cagccagtag cctatttcag cggttttatt cagtggttca atacgattaa acgagataac
     4381 gccgataagt tcatcttctt tgaaaatcat gaacattttg gcatagccgc gttgatgcaa
     4441 catcacatta ccctgcaccg tttttcgcgt gtcctcttca ctctgaacga actgcggcca
     4501 gtttagcgac tgctgtaacc aggttttatt tttacagatt aactgataaa gaggagtgac
     4561 gtgattttct gcaacagcat gtaattcaag tgattcgctt acttttatcg tttcagtcat
     4621 cttatctcca gtcaatccat gcagttgtta aaaaagccgc ctggctggcg gcttaggaaa
     4681 gtttacacct ctttgacgct ctcctgctgc aggattttga gcatctcatc ttttaactgt
     4741 agcttttgtt ttttcatgcg gaccacttcc gcattgtacc ctcgaccgtc ggaaccttcc
     4801 tttctggcaa tttcatgatc aagtttattg tgtttatcga acaaggacat aaagcgagga
     4861 ttttcgtttt tcagacggga tattaaatct cgatattctg gaaacatacg cactccctgt
     4921 tagtggctga caaggtgtat ctgaaataca caccatcaga ttactcattt aacttaggga
     4981 aaaatgcgag cgggatcgtg tttaaccaga cggcacctcc cgaagaaggt gccgaagaaa
     5041 aaacgattaa ctcatcacgc ggtcgtcaac atactgacgt ttatccggcg ctggcgggaa
     5101 atactgatac agccatgttt cactgatagc gtcgccctga caacgcagat acatccgcat
     5161 atcgaccgga tcagtggagt ccgaagtcgg ataccagtca aactgaatac gataaccatc
     5221 aatgggttca atatagagaa tttcgatttg cttcgcttcc ccactggaaa gcgtaatcac
     5281 cggctcaatg ccttttggtg cggcagcttt cagatcacca ccaacgaaat cgacggcaaa
     5341 acgacgcgcc catttttcgg gatagtgttc acctggtgcc caaccttccg ggaaaccgcc
     5401 catgccggta cgcgtcgcca taacgcgcgc taatgggcaa tgaacaggcg gttgcgcact
     5461 ccagtacaga cgatactgga atgcaaactc atcacccgcc tttacagctt tttctggctg
     5521 ccagaagcag acaatgttat ccagcgtttc gcccgttgtt gggatttcca tcaggccgat
     5581 ggtgccctta ccccacttgt tacgcggttc cacccacaga cttgggcgtt tgttatacca
     5641 gcccataatg tcctgataat gggagaaatc acgatccagt tgcaataaac caaacccttt
     5701 cgggttgttg tcggtgtaag cattgaactg caatttttgc ggattattca gcggacggca
     5761 aatccactcg ccgttgcccc gccacatgga cagacgatca gagtcatgaa tttgcggatg
     5821 aatggtgtcg cacatccgac gttcattagt accgcagctg aacatactgg tcatcggcgc
     5881 aatgcccagc tgtttaatgt ctttgcgcgc atacaggtga ttttccacat ccataatcac
     5941 ctgacttttc tcacaatgga tagtgaactt ataggcacca gtaatgctgg cgctatcgag
     6001 caacgcataa acggtaaagg tagttgcccc cggttttacc gtatcaaacc agaaggcggt
     6061 aaagtcgggg aactcttctt tactgtcggt gtaagtgtca atcgccaggc cgcgggccga
     6121 caaaccgtat tgatatgtgt catcaacggc gcggaaataa ctcgctccga ggaatgatac
     6181 tacatcacgg cgcgccagtt cgggggcttt aaacacgcga aaaccggcaa agccgagatc
     6241 gctttgccct tctaattgtt tggtatcaac acctgcatcg ttgtatttga acaactccgg
     6301 gcgaaagtga atttcacgcg ccagatgtgt tgctggatct acagaaaaca tacgaacgcg
     6361 gcgacggaat cccattccca tatggaagaa ctgagcgtcc agttgacggt tctcaacgtt
     6421 atgccagagc gatttttcgg cgtcgtattg aatactgtta taagcctgcg gcgtcattgt
     6481 cgccagcgtg tcaggtaacg gacgaggcgc accacgccac gctgtttgcg ctaagtcgtg
     6541 cgccattgac tgtagaatgg agaagtcaaa acgctgggtt tgcccgtcgg caatatcaga
     6601 atctgccgcg aatgccgcct gagaaaaaag agaagcaatg ccgctggtac cgcacacggc
     6661 ggccatagcc attgaacctt taataaatcg tctacgatcc atacctgaaa gtgagtcctt
     6721 ctggtgttgt gaatggtccc gcgctggtcg ttttcagcgg caagaaagtg cttatttatg
     6781 accgctcact ctagacaata tttattaata atccaattgt tgaactcaga gaatatgcat
     6841 aaatcggcaa tttttttatg tcgatgggat gagcctgatt cgtttgtaaa atcaggcaaa
     6901 tttttgcaaa ggcggatggt cagaatttaa agcaaaccac agcgacgttt actgcgttct
     6961 tccgcttcct gataaagctg gaactcatcc aaaaccggac aacccaacgc ctgctcaaat
     7021 gcctcgcgac tggcattacc gtgattgcgt gcctgcgttt cattacccag atttgactgg
     7081 aagatccccg ccgcgctaac gggcaagaaa tcttcatagg taatgggttg cgccgcgacc
     7141 caaccacgtt caattaaggg ctgtggatcg tctccgggat gaatcgcctg acgatgcgcc
     7201 tcacctgaag gcgtcagacg gtaccggaac catgccagcc cttgctgacg cattaaaaac
     7261 tcactgtcag gaaaagtgcg gaaggtttcc tgtaaatgca tttggtgagt gagattatcc
     7321 tgcccggttc cagcgttacg cagaagatca tcatacagtt gtcgcccttt cggcgttaat
     7381 gccacgccac gctgctcaat ttcaccaaag cgcgcggtat gcgtgccctg tttctgcccc
     7441 gcaaacaaca ccgtctcttc cagtgcttta aagctggtct ggcgtagtaa aatcggtacc
     7501 tcgcggcgcg gcggcccctc gatgagaatt ttgggttcaa ttccgcattc aggcatcatc
     7561 gactgcaccc ggtcaatatc cagcgtacgt ggcgtcaggt ggttgatatg gcatccagga
     7621 aaacagacca catcagcaat caaccgatgt tcgttgtgca aggcgcgata ggtttcttca
     7681 tctaccgttg ctgactggtg ccagcgaaac gtttccaggg cttcctgcac aaactcctgt
     7741 gcctgtgttt cgttaaaacc gccctgctgc tcatattcct ctaacagttg tcgacaacgt
     7801 ggggtgaaga tatcgcgctg acgcagaatc tccgccgctt tctggcgcaa aatttcgttc
     7861 tcgataagct caaggcggag taaggaggta aaaacgcgga agggattacg cgccagagaa
     7921 gcatcatcaa tgggccgaaa tgctgtcgaa tgtaccggca cccctgcctg cgagagatcg
     7981 taatagctca ccgggtacat ccccataatg gcaaacatcc gccgaagagt agcaagctct
     8041 tgtgcagtcc caacgcgaat cgccccatga cgttcaacat tcagtcgcgc cagctcgtct
     8101 gcatttacca ttttttcgtg cagttgagga ttgttttcca gcacagccag attcacatca
     8161 gctaccagtt ccagcagcgt gccatattgc ggaacttctt gctggtacat ggctgacatt
     8221 gcctgcgaaa actgttcccg aatctcatcc gccgtgatgc tgttcgccat agtgtctgcc
     8281 tccagtggtc agtaatctgg agtgtaggta accgcattca ctctggcggg aagaatttac
     8341 aaactgtgat ctcgccgcga aaacatcaat attatccatt ttgctgtaac acaattgctt
     8401 taattgttaa taatattttg caatcaagtt atcataatca aacaacttca cttgtcagcg
     8461 acaccgcttc gtttttaaca tcgcttatgg aaaaaaatag tctgtttagt cagcgcatcc
     8521 gtttgcgcca ccttcataca ttcgtagctg tcgcacaaca aggaactttg gggcgcgcgg
     8581 ctgaaaccct taatttgagt caacctgcgc tctctaagac attgaatgaa ctggagcagc
     8641 tgactggcgc tcgcttgttt gagcgtggtc gtcagggggc gcaacttacc ttacccggcg
     8701 aacaattttt aacgcatgca gtcagagttc ttgacgccat caacactgcc ggacagtcgc
     8761 ttcatcgtaa agaaggtctt aataatgatg tcgtcagggt tggtgcacta cctactgcgg
     8821 cactggggat attaccttcg gttataggtc agtttcatca gcaacaaaaa gagacgacct
     8881 tgcaagttgc gacaatgagt aaccctatga ttctggcggg tttaaaaacc ggggaaatcg
     8941 atatcggcat tggtcggatg tcagatcctg aactgatgac cgggcttaat tacgaactgc
     9001 tgtttcttga atcgttgaag ctggttgtcc gccctaatca cccgctactt caggagaacg
     9061 taacgctaag ccgggtgctg gaatggccgg tcgttgtatc accagaaggc actgcgccac
     9121 gccagcattc agatgcatta gtgcagagcc agggctgtaa aattccttcg ggttgtatcg
     9181 aaacgctgtc tgcttcgcta tctcgtcaac ttacggttga atacgattac gtgtggtttg
     9241 tcccttctgg cgctgtaaaa gacgacctgc gtcatgccac gctggtggcc ctgcctgttc
     9301 cgggacatgg tgcaggcgaa ccgattggaa tactgacccg cgtagatgcg acgttctctt
     9361 ctggttgcca gttgatgatt aacgctattc gaaaatcaat gccgttctga aaggtgaagg
     9421 gatctgtcga tccctccttg aacattttca caccgtagcg aaactaactg gttcacccgc
     9481 tccgcgaggt tctgccgaca cagaatgttt gtgcagacgg aatacatcca ccgcctcagt
     9541 taatcgtgcc gcctgttctt caagggacac cgccgccgct gaggcctctt ctaccagaga
     9601 agcattctgt tgcgtcacct tatccatttc agaaatcgcc tggctaacct gcgttatgcc
     9661 tctactttgt tcatccgagg cggcggcgat ttcctgcatg atatgtgtga cactcgcgac
     9721 ggcatcaaca atagtgctca tggttttccc tgccgttgcc acctcatccg accccaggtc
     9781 aattaacctg actgattcac tgatcaagcc ttcaatctct ttcgccgctt gggcgctgcg
     9841 gcttgcgagt gtccgtactt cgctggcgac aacggcaaat ccacggcctt gctcacccgc
     9901 tcgcgcggct tcaacggcag cattcagtgc cagaatattc gtctggaaag caatactgtt
     9961 gatgacggcg gtgatctcgg aaattttctt cgaacttgta gagatagcgc ccatcgtttt
    10021 tactacaccg gaaaccgtct gcccgccatc gctggcttta atagaagcct cttgcgccag
    10081 tttgctggca tgatgcgcgt tatccgcatt ctgtttcacc gtcgcagtga gttgctccat
    10141 gctagcggca gtttgttcga tagccgccgc ttgttcttcg gtgcgagatg acaggtccgc
    10201 attgccagct gaaatttcgc tggtgccacg ataaatctct tccgcaccct gtcgaacagt
    10261 ccctactgtc atccccagtg aatgctgcat ttgctgtaaa tgacgactta agcgaccgat
    10321 ttcattacga cccgccggtt catcattcat cgtcagatcg ccactggcga ttttttcaat
    10381 ccgttgtgcg gcatgttgca gtggacgaat gacgatccga cgtagcacca taaatgttat
    10441 cagcgtcatg accagggcaa gcacaaacgc gccaatcatg aacatcccac ccaggcgggt
    10501 gcgctgatgg gccagttccg ccagttggtt ggctctggtg ctacgtatct taacggcttt
    10561 gttcaaaata tcggtataag cattatccag cggtcggatc tgctcacttt catgattgat
    10621 aatcgcttca aacatgccat ttttggcata tttcaacata ggttgcatac ccgtgatata
    10681 agcctgaaag cgttgattta attcagtgtc gagggcttca tcagcaggtg ttttcaccgg
    10741 tcgattctga taagcacgat aaccttgctg cgactgttta atctccgatt cggcttgcgc
    10801 aatatttcgc ttcattgctt ccatttccgc aatacgactt gcagccccgg cctgaatcat
    10861 attgatccgg gctgaacgca aaaaatctga actgttcgct aacccggtcc gaatctcaat
    10921 ctccgcagta acatcacgct gatcgcgatc ggcctgccag aggaaatagc cagccagagc
    10981 agaacttaat gcgaatagaa ctaagatacc gccaagaatg caggcaaata acggaaccag
    11041 cctgatgtga tgcaaaaaac ctaatcgatg tgagggagtt gtattcatta ccgttgtctc
    11101 tcgtccaggt ttactcctga tagaaagtca tcggcacttg acggtaatta cttatgacca
    11161 aaaacctctt tgcgtcacat ttttacaaca attattgcag agaaaattaa tgctattgga
    11221 atgaagagag taacgaatgg ctaaactggc taacccgaat gacgaaaaat tctggaaaac
    11281 agcacgcaat ttcgtgaagt gattattttc gtccctaagg aaacctcata ggatgcctgc
    11341 gggtgcctga gactttctgt tttcaggcta accgggaatc agaaagacga aggccccgac
    11401 cgatatttct atcaaccgag gccaacgaat gaacctagca attcagatct tagcctctta
    11461 cccgccatcc ggcaaggaga aaggctatga agcacaaccc tctggtggtg tgtctgctca
    11521 ttatctgcat tacgattctg acattcacac tcctgacccg acaaacgctc tacgaactgc
    11581 ggttccggga cggtgataag gaggttgctg cgctcatggc ctgcacgtcc aggttagggc
    11641 aagcgcgggg attttccccg cgcatctgac tagcagattc attttctcat ggcacccctt
    11701 ttacacttca gttagtgcat gtttttttcg aggcactaat agaaccatca ttacagacaa
    11761 atttgccgcc tgccgtacag tgtgagatcc cccctttcga accagaacag ggtttgcgcc
    11821 cggcatcggc ataaaaggaa acaagcgttc ccataaacaa aataagtgcc agttttttca
    11881 tataacctcc atggtttatc gaatcttatg ttcgatttga acgttatacg ctggtttatt
    11941 ttgataccgt tgatgagatc aaagatcggg tgaattgatt aaaaataagc cagatggacg
    12001 tatgggcaag gattatggtt ttatttgtca tacaaataaa tataataggc gcttccgatg
    12061 tagacccgta ttcttcgcct gtaccacggg tcggttttag tacaggcgtt ttctttaaat
    12121 atccttcagg aacgtttacg cggcatcatg cgtagaagtg tgttgtcctt ccagaaatag
    12181 tggtgtgcca gtgcagccgc agcgtgcaac ccgatgacaa aatatcccag attcgccagc
    12241 gtctcatgcc acgactttaa gctatcaacc cgttcgaaat tggcctctga agcgtaaggc
    12301 atcgtcaaac caaacgcaaa ccacgggttg ccccggttat acatcatcac caaaccaatc
    12361 actggcagcg caataaacag gagataaatc accaaatgtc ccaaatgcgc cagtcccgtc
    12421 atcatcggtt ttggcttagg tataatcggc ggggttgggt atttcagtct taacaaaaga
    12481 cgaaccacca tcagcacgag aattgagata ccacaggaaa catgaaccat gttgatgagt
    12541 ggccgatcgc tacgtgggaa gaaaccacga aactccattg cgcaatacgc tgcgataacc
    12601 agcaaaaaga ccagccagtg aatgctgatt tgtaaccttg agtatttatt ttccataaca
    12661 tttcctgctt taacataatt ttccgttaac ataacgggct tttctcaaaa tttcattaaa
    12721 tattgttcac ccgttttcag tacgttacgt ttcactgtaa gaatggagat tgcccatccc
    12781 acaatcctgg tctaagcctg gaaaggatca attttcatcc gaatgttcct gacaggagaa
    12841 aaccatgagt aaagttggta ttaacggttt tggtcgtatc ggtcgactgg tgttgcgtcg
    12901 attacttgaa gtcaaaagca acatagacgt tgtcgctatt aatgatctca cttccccaaa
    12961 aattctcgcc tacctgctga aacatgattc aaactacgga ccattcctct ggagcgttga
    13021 ttttacggaa gattcactta tcgttgatgg gaaaagtatc gcggtttacg ccgaaaaaga
    13081 ggctaaaaat attccgtgga aagcgaaagg tgcagaaatc attgtcgaat gtactggctt
    13141 ttatacctcc gccgagaaat cgcaggcgca tcttgatgct ggtgcgaaga aggtgttgat
    13201 ttccgcccct gccggtgaaa tgaaaactat cgtttataac gtcaatgacg acactctgga
    13261 tggcaacgac accattgttt ccgtggcgtc atgcaccact aactgtcttg cgccgatggc
    13321 caaagccttg catgacagtt tcgggataga agtcggcacg atgacgacca ttcatgccta
    13381 tactggcacc cagtcactgg tggatggccc acgtggtaaa gatttacgtg cttcacgcgc
    13441 agcggcagaa aatatcattc cccacactac gggggcggca aaagccattg gtctggtgat
    13501 cccggaactg agcggcaaac tgaaaggtca tgcgcaacgc gtgccggtga aaacaggttc
    13561 ggtcactgag ctggtgtcca ttctcggaaa aaaagtgact gccgaagagg tgaataacgc
    13621 acttaaacaa gcaaccacca ataacgagtc atttggttat accgatgaag aaatagtctc
    13681 ttccgatatc attggcagcc atttcggttc ggtgtttgat gccacgcaaa cggaaattac
    13741 cgccgtgggc gatttacaac tggtgaaaac ggtcgcctgg tacgataacg aatatggctt
    13801 cgtcacgcag cttattcgca ccctcgaaaa attcgctaaa ctctgacgcg cacaggcgga
    13861 ggaaaaaacc tccgcctctt ttactcatta cgactgtaaa taaaccacct gggtctgcag
    13921 atattcatgc aggccatgtt taccatctgc gccgccaata ccggatttac gccatccggc
    13981 gtggaagcct tgcatagctt cgaagttttc acggttgatg taagtttcac caaacttcag
    14041 ccctttaatg gctttcatcg cgacgttcag attttgggta tagattgatg aggtcaggcc
    14101 gtaatcactg tcattagcca ttgagatagc atcttccagc gtgtcaaatg cgaccaccgg
    14161 cagaaccggg ccaaaggttt cctcatgcat aatcgacatt tcctggcgaa catccagcag
    14221 caatgtcggc ggataataat atcctttccc gtctactgct ttgccaccca acgccactct
    14281 cgccccttct tctactgcgc gcgccacttt ttgctcgacc ctttccagcg ccgcggcgtt
    14341 aatcaacggc cccatcgcaa tgtcgttgcg ttcagcgggg ttaccaaatt gaaccgcctg
    14401 catcgcttca cccagccgat tgacgaactg atcataaatg cctttctgta cataaacacg
    14461 ttctgcacag ttacacactt gcccactatt aatgacgcgt gaatcaacga tggctttgac
    14521 tgccagttca agatcggcat cgtccattac gatagctggt gctttaccac ccaattccag
    14581 gcacactttg gtgatgtttt tcgccgcagt cgccataatc ttctcacctg cagagacgct
    14641 gcctgtcata ctgaccattg cgacctttgg gttacccgcc agttcttgcc caacggtttc
    14701 accacgcccc agtacaaggt taaacacgcc gcgcggaagg cctatttcat cgacgatttt
    14761 ggcgaatgca atcgcattgt ttggcgtaaa ttcactgggt ttaatgacga tggtattacc
    14821 ggtcaaaaga gcgggagcca ttttgcgggc aatgaggaag aacgggaagt tccacggcag
    14881 aatgccggta gtcacaccaa gcgcacgttt aaacaaaaga atattttctc ctggacgatc
    14941 gctttgaata atctcgccct cgtaacgccg tgcccactcc gccatgtaat cgatatagtc
    15001 ggcagtaaaa gccacttcga cttcagccag ctgctggatc ttgccccctt cttcaacaat
    15061 cagcgcactg atttcactgg cgcgttcgcg gatgccggcg gagattttgc gcaaccaact
    15121 ggcgcgttca atagcaggca acgcttccca ttctggttgt gcacgttctg ctgcatcgat
    15181 tgccttacgg gcatcctcgg cctgaccatc gggtatgcgg gaaatgacag cctctgcagc
    15241 agggtttacc acatcaatcc atgcgtctcc acgccaggta acaaactgtc catcgatata
    15301 cataggatgt tgaacgggta ctgacatggg cgactcctgt gatttatatg tttgttttcg
    15361 gtgaatgcat tgttaattaa catcaatcat cagagagacg gcatcgtgaa aggacaattc
    15421 tgtgaagggc ttcataaaaa caagtgtaaa aggaataaaa ggcagaaata aaagtcagga
    15481 gtcatgcccg tttgcaaaca agtggttcac aaacgggcaa tttttaacgt aacgagcgac
    15541 tttccatcgc ctcgatgagc accgcatcat gtttcagcgt ttgccatgca tggatgactt
    15601 ctgccggaaa atcaacgtga acgataaaat ctcgcccgcg gggaccgtag ccatcgctat
    15661 catcgcgtaa acgcggcagc tcgccagtga gtagtgagag ataacgctca accggccata
    15721 atccttgtaa ctgattaata aagattacac tgtctgcgtt atttcctaac tgaggaacga
    15781 atccgggata acttaaccag cgtggtgcat cgggattgtc cccagtcata cggcggaacg
    15841 tcgccatcgt gcgccgctgc atggtggggt cctgaacaac gatagccgta tgaactcgtt
    15901 ctacggcctg attcaatagc gcgatgctaa agcgtgcgtt ttcaccgcag tttgttgact
    15961 ggtcttcaat ccagattttt tcatgcggaa tgtgccagaa ctgatgagcg atatccgcca
    16021 ggatggtcgc ttctgctctg ccagtggtgc ggatagtgtt gtagtgcgga tgctgtgcga
    16081 tggcgctata caaaaaagtt gtcgagtgac cgataccacc actaatcagt aaaggaattt
    16141 gctgatcgcg agcaatctta catgccgcgt cgatagtcgg cataaccgca ttgcctgcaa
    16201 ggatcacgca atcggcctga tacggcacct caccggagaa atcatcctgc gccagccact
    16261 gtccgataac atttatggca tctatagttg ccggcgaaag cgtcggaaac ggggtaatgt
    16321 tcatggttcc tccttatcgt tcccttaaga atactgcgtg agataatagt gagaagcgga
    16381 gcactctgaa aggaggaagt cgtatgtcct taaccgccac tccgcaagcc tgacatatct
    16441 tttaaggaaa tgctgtagat aaacattatt catcttgcag cagagaattg tatttttctg
    16501 aattcctgaa tttttaccct taccgtttta gaattaataa aaaacccggc gctaaatgct
    16561 taccgggttt tttctttatc aggcaaatag cagggttaac cgctaatctg ctccatcgcc
    16621 tgcaaaatac gcttatctga aatcggataa ggcgtaccaa gttgttgagc gaagtaacta
    16681 acgcgcaact cttctatcat ccaacggatc tctttcacgt cttcatcctc acgacgtgcg
    16741 ggcggcagtt tgttgatcca ttgctgccac gcctgctgga cgttttcgac tttcagcatc
    16801 tgagcacggt cgcgatgtgg atcaaccgcc agtttttcca gccgtttttc aatcgcctgc
    16861 aaatatcgca gcgtgtcgcc cagccgtttg aagccgttac cagtgacaaa accgcgatat
    16921 accaacccgc ccatctgcgc tttaatgtca gaaagcccca gtgccatggt catatccacc
    16981 cgccctttca gacgtttgtt gatattgaac accgcagtga ggatttgctc gacctgcttc
    17041 gcaatatcca ccaccgtgtc attcagttcg gcgcgcactt tttcatgcag cgcagcaaag
    17101 ccttcttccg tccagaccgg gccaccattg gcgtcgatca attgatccac accgcaggag
    17161 atacagtcgt cgatcagctc cagcactttg ccatacgggt taaagtacag tcccagcttg
    17221 gctttgttcg gtaacttttc atgtaaatat ttgattggcg atggaatatt cagcagcagt
    17281 agacggcgaa gaccgttcca cattgcctgc ttttgctcca gcgggttatc aaacagtttg
    17341 atcgccacac tgtcgcgctc atccaccagc gccggccacg ccttcacttt gtagttgcca
    17401 cgcttctgtt cgtagctttc cggcagctga ccaaaactcc agatatgtaa gccgctctgc
    17461 tcgataccgt catccgccac cgcagatagc gtttcctgca ctttgccttt cagcgcatct
    17521 ttcagatctt gtagcgagcg cccttctttt agcttcttgt ttttgtcatc caccacgcgg
    17581 aaggtaattt tcaggtgatc aggcacctga tcccagtgcc agtcttcgcg gtcaacggta
    17641 acgccggtca tccgccgtaa ctcgcgctca aggctgtcga gcaacggtaa ctccagcggt
    17701 ttgacgcggc ctaaaaacgc ttcggcatag tttggcgcgg gtacaaaatt acggcgtacc
    17761 ggtttcggca acgatttaat cagagcaatc accagttcgc ggcgcagacc ggggatctgc
    17821 cattcaaacc cgctttcctc aacctggtta agtaacggca gcggaatatg tacggtcaca
    17881 ccgtcagcat ccgcgccggg ctcaaactga tagctcaaac gcagcttgag attgccctga
    17941 tgccagaagt tcgggtaatc cagcttgctg attttttctg cgccctcttt gatcaacatg
    18001 cttttttcaa agttgagcaa atcaggggtt tcgcggctga cttttttcca ccagctgtcg
    18061 aagtgacgag cggagattac atcgtggctg atgcgctggt cgtagaactc aaacaacgtt
    18121 tcgtcatcaa ccagaatatc gcggcgacgt gatttgtgtt ccagctcttc cacttcggcc
    18181 cgcagtttca ggttttcacg gaagaatgcg tgacgcgtct gccagtcacc ttccaccagc
    18241 gcatggcgaa taaagagttc acggcataac gccggatcga tctggctgta gttgaccttg
    18301 cgcgcggcaa caatcggcaa accataaaca gtgacttttt ccgttgccat caccgcgccc
    18361 tgcgcccgtt cccagtgagg ttcgctgtag gtgcgtttaa tcaaatgctg agcgacaggc
    18421 tccacccatt ccgggtcgat acgcgcagca atgcgccccc acaggcggct ggtttctacc
    18481 agttccgcca ccattaccca tttcggcggt tttttgaata aaccagaacc gggaaagatg
    18541 gagaaacgcg cgttacgtgc gccggtatat tcttgtttat cggcatcttt catgccgata
    18601 tgggaaagta aacccgtcag caacgcaatg tgaatttcgc gatactccgc cggttcgctg
    18661 ttaaccggaa tgccaagttc tttcaccacc tggcgcaact gggtgtagat atcctgccat
    18721 tcgcgcacgc gcagatagtt gagataatcg atacgacaca ggcgacggaa ggcgttggaa
    18781 gaaagcgcct tttgctgctc gccaagataa ttccacagat tcacaaacgc gaggaagtca
    18841 gactctttgt cgtggaagcg acgatgtttt tcgtccgatg cctgctgttt atccatcgga
    18901 cgctcgcgcg gatcctgaat ggaaagcgcg gacgtgataa tcatcgcctc acgcacgcag
    18961 ccatgttttt gcgcttccag caccatacgc gccagacgtg gatcgacagg caactgcgag
    19021 agctggcgac cgagcggcgt cagtttatag gcgctggcct gttcatccgt ggtgatcgcg
    19081 cccagttctt cgagcagacg cacgccatcc tggatattgc gtttatccgg tgcttcgaca
    19141 aacgggaacg cagcgatatc gcccagcccc agcgcggtca tctgcaaaat aaccgaggcc
    19201 aggttggtac gcagaatctc cggatcggta aactccgggc gcgaaaggaa atcgtcttcg
    19261 gaatagagac gaatacagat cccttcggac acacgcccac agcggccttt acgctgattg
    19321 gcagacgcct gcgaaatggg ttcaatcggc aaacgctgca ctttggtgcg atagctgtag
    19381 cggctgatac gtgctgtacc ggggtcgata acgtatttaa tccccggtac ggtcagcgag
    19441 gtttccgcga cgttggtcgc cagcacaatg cgccgtccgc tgtgcgactg gaacacgcgg
    19501 ttctgttcgc tgttcgaaag ccgcgcataa agcggcaaga tttcggtatg acgcagattt
    19561 agcttgttca gcgcatcggc ggtatcgcga atttcccgct caccactcat aaagatcaga
    19621 atgtcgccag ggctttcctg actcagttcg tctacggcgt caaaaatcgc ctgcaactga
    19681 tcgcgctcgg tgtcgtcggc ttcttcaaca atcggtcgat agcgcacttc caccggatag
    19741 gtccgaccgg agacttcgat aatcggcgca ttattaaagt ggcgcgaaaa gcgttccggg
    19801 tcgatagtcg cggaagtgat aatgattttt aggtcaggac gccgcggcag caactctttc
    19861 aaatagccga gcagaaaatc gatattcagg ctgcgttcgt gtgcttcgtc aataatgatg
    19921 gtgtcgtact gcatcagcag gcggtcttgc tggatctccg ccagcaggat accgtcggtc
    19981 atcagcttga ccatcgtgtt atcactaacg tgatcgctga aacgcacttt gtaaccgatg
    20041 caaccgcccg gctccgtttt cagctcttcc gcaatacggt tcgccaccgt tcttgccgcc
    20101 aggcgacgcg gctgggtatg gccgatcagc cctttaatcc cgcgccccag ctccatacag
    20161 attttcggta actgagtcgt tttaccagaa cccgtttccc cggcgacgat caccacctgg
    20221 tgatcacgaa tcgcttcgag aatgtcctgt tttttctgac taaccggtaa attgtcagga
    20281 taagtaattt ccggtcgtgc cgcttcacgc agcaggactt tccctgccgc ctggtcaatc
    20341 tctttcgcca tctcctggaa aatggcctgt tgtgcatcag gatttttaac cttcttaaca
    20401 ccgtgcagac gccgagaaaa acgcagtctg tcgcgcagca tcagcgaatc cagccgctgc
    20461 tgcaaggccg taaaggtcaa tttttgttgt tctgtcataa cgttagtggg cagtagtctg
    20521 cctgattaat tttcttttaa atgatagata tagagtacca catcgcggct ttctatgcgt
    20581 tgttcaataa attcgaacat aggcttcgat ttattgcgct atctctgtgg caggattgtg
    20641 aataaagtgt cagcaagcaa cggggcatcg cccattcaaa catctataag gaaacaccat
    20701 gagcaaggta ttagttctta aatccagcat cctggcaggg tactctcagt ctaatcagtt
    20761 gtccgattat tttgttgaac aatggcgcga aaagcactcc gctgatgaaa tcaccgttcg
    20821 cgacctggct gcaaatccga ttccggtact ggatggcgaa ctggttggtg cactgcgtcc
    20881 gagcgatgcg ccgctgactc cgcgtcagca ggaagctctg gcgctttccg atgagctgat
    20941 tgccgagctg aaagcccacg acgttatcgt tattgcggta ccgatgtata acttcaacat
    21001 ctcaactcag ttgaaaaact attttgacct ggttgcacgc gcaggcgtta ctttccgcta
    21061 taccgagaac ggtccggaag gtctggtaac gggtaaaaaa gccatcgtta ttaccagccg
    21121 cggtgggatc cacaaagatg gaccaacgga cctggtgacg ccgtatctgt ccacgttcct
    21181 cggctttatc ggcattaccg atgtgaaatt tgtcttcgcc gaagggatcg catacggtcc
    21241 ggaaatggca gcgaaagcgc agtctgacgc gaaagcggcc atcgacagca ttgttgctgc
    21301 ataaccggac acgatcttga tgtggactgt agttcagtcc acatctcaat ccacttacct
    21361 gttttcccat aatttcagca tcgctttgtg atcttcctta agcacaatat gcgaacgtct
    21421 ggctcgaata taactaatcg cttcatcaac ggttttacag tgtccgtaac ataacaacca
    21481 tgccgccact accagcgcac tgcgcgataa tcccaacgcg caatggacca gaacgctgcc
    21541 ttgctcttcg cgtaatgttt ccagcatcgc cacggcctgt cggagctccc cctcttccgg
    21601 aaccaccaga tccagcatcg gtacacagaa atagagtcga tcttttgtcg ctcgtccccg
    21661 agggaattca aaggtgacgt ccagaaccgc attctgtgcc ggaatatgtc gtggaaacgc
    21721 ccctaaataa acaccagcag taattttgct caccggctcc aggcgacgac aaaaccaacg
    21781 catagacagc cacatcccga tgcgccaggg caatgtcagc cagtaaacgg cgggcgggag
    21841 tttcccctga ctatctttcc ctgttgttat cgcgccaagc ccaccgtacc cacggccaat
    21901 aatgagtagc gataatactg gccaacataa ccagactgac caccataact gaatcatcac
    21961 cattagctcc atcaacacaa tgcacgagcc cgcgcctacg acatatggca gtgctatttt
    22021 gattcgacgt tgatcaggtt tctgataatt ccaacgacga tcgacgggca ccatccagtc
    22081 aatcaacata cctaccgcca gccctgtgat gacatcaata aaatgatgct gccaggtcgt
    22141 cagagtagaa atagcgatga gtaaaaacca tccgccacag actttacgcc acctctcagc
    22201 cagatgctga cgaaagtgac gccaaagtag ccagcagaga ataatatgca gcgaaggaga
    22261 ctggttataa ggcagatcaa acagttcaag ttgcgaaaat agccatcccg tcaccccact
    22321 cacttcagga cggataaaac taaacttcag cggatagagc aaaaaaccgc agcaggccat
    22381 taccgttgcc agaataagcc ggtggacaag tcggcgctgt tcgaatgtcg tgctacaaac
    22441 gaacagcgaa aatccatata aaagatccag actccagtaa ggaacaatag tccagggaag
    22501 aaaagggatc gccgtttccc aaccgaatac ctgactgggg atatcatggc tgttaaggtc
    22561 ctgaaccgcg gtgaactgat taagagatcc ataggtgaag aagaaaaacg gggccagcaa
    22621 caataaccag ccagcgcctt gtagcaatac gttccgacgt tcagttatca cgacgcaccg
    22681 ccatcgaaac cgtaaaaata ccccactcat caatccgttg tgtgcattta tcaaatccgg
    22741 catcacgcac gagtgaatcc atctcccctt gcgaacgcac gcgcattacc cacggtttac
    22801 catctttatg actggttaac accccggcaa tcatctccag ttgagggtgc cacggctgcc
    22861 cggtgtagat gagaatgccg cccggttcga tggcattggc aagacctgcg agtgagtttt
    22921 ttacctgctc attttcggga aaaagctcat acaggccaga gacaatcgcc agcgtaggcc
    22981 gcggagttaa cgcgctgagt tcctccgggt taaacgcatc gccctgttca aaacgcaccc
    23041 gcccagacat tccccgttgg gcaatcatcg cttgcccctg tgcaacattt aactcactgt
    23101 aatcacgtaa caaaatatcg cttacggcag gctcgtttgc cagcgcatcc agtacatagc
    23161 gcccatgccc tgcggcaatg tcaaccacgc ggacggctaa acctttggcg tggagatcgg
    23221 caacggcttg tttaatcagt atttgtaaat gggttttacg ctggcgaata ccgcgccagc
    23281 caacactgtt caggtagatt ttgtcaatta agcgcccgaa tgcgttactg ccttgcggtt
    23341 gattttgata gacataatcc agggaactgc ctgaatcaaa gccggtgctc attccgagat
    23401 gcaggcccgc agagtgcgcc ccgaacaatt tcatcgcctt tcgcataaag cgatacgcca
    23461 aatcaaccgg cgataatggc acgggtccac ctgaaaggag ccgccagcga tccgctgatg
    23521 gtccagtgcg gtcttcatgc tgataatcaa atttttgtga cttgttagca tataaacgac
    23581 taataaagct ttgcattttt tcaaatgcct gcgccctgtt ctcttcaccc aacgtgtcgt
    23641 gataaaagcc tggcagcaga tgcagctctt tcagagggct acgtaatctc tgataaaaat
    23701 caatctgcgg ttggcgatgc accacatagt catcgcctga tatcagaagt tgcgtgggga
    23761 gcgtaatcgc cgccgcatct ctaataatac gttcagacgt tttgtagaga tcgagcaaga
    23821 tattaacggc aatcgcccgt gtgatcagcg gatcattatt gaaactcgcc acccgttgcc
    23881 gatcgtgggt caaatagcgt cctttcacat aggaattaat aaaaaacaga ccacgcagac
    23941 gatgccataa cgccagcgca ggacgtgcca gcggtacata caatttaacc ttaaaggccg
    24001 gagaagccag caccagcccg cgaattgcag gcgcataatc atgaacccat gtggcaacca
    24061 gcactgcgcc gacgctttgc gcgatcacta ccacctcatc cagtccgacc tggctatcgc
    24121 tggcagcaaa acgaacaaat tcatcgacat cctgcactga acgcgcaaga gatgggctat
    24181 aaccacgcgg ccccgaagtt tgtccatgcc ctcgggcatc ccaggcataa aaaacagtat
    24241 ctggcatcgc cagttcatca acgagatgtt gtagacgacc agaatgttca tgaccgcgat
    24301 gaaataagac gatgaccttt ttagccccgg gctgtaaagc gggccagtgc cgataaaaca
    24361 acgctgtatt atcactgctg gtaaaaaaat gttccccatg gatgcgtgaa ttttccattt
    24421 tctctatttt ccttcagtaa cagcagtagc gaataaaata aaaaaatacc ggagcggtga
    24481 aaattaatga gtcaatcctg tcaaggagtc cgccatgtcc tggtaatagt ttcccactat
    24541 ctttaacacc aatatctcgt ttgatggctg acatcacgac atcgccgcaa aaaccactaa
    24601 taccaattaa caaacccgct aataatgcct gtaatgtatt tagcggtgtc agcaacggtc
    24661 caataatcag tgacgcaatc atggtggtga ttacgccccc caccagacct tccagcgttt
    24721 ttcccgggct gactttgggg accacttttc ttctgccgca ggatttcccc cataaatact
    24781 gtgcaatatc gtttgactcg gttaatgcca aaagaaataa caccagtaac gccccgccct
    24841 ggatatttgt ggttggcaac attaataacc aggcggcatg actcaaggcg aaaacggtca
    24901 ttatccagcc ccaaaaaatg gcgctcacgg tatgcagaaa accggagggg tcccccataa
    24961 aaacttgccc ggtggctaat attagaaacc cagccaaagg tataaataat agatacagct
    25021 caaagcaatt aaaaccgata aacaaatagt taagaggaat aacccaatat aaccaccgcg
    25081 gaaaatgtac agatataagc gtacaatatt ctttcaatgc cagaaaactt atcagcgcaa
    25141 aaaacgtcaa cgtcatccag cgtggacctg aaatcaccag tgaaaaacaa atgacgatgc
    25201 cccaccaggt acgaatacgt aatgtaactt catcctcttt tctctccggt aaccgccata
    25261 gcagaaaatg gtttatcagc gttgctaaaa ttaacaacgc gaaaagtgtt gccagagatt
    25321 tttccagcat tatctttcag cgctcataag aagcacactc cgacaacgat taatcgcagt
    25381 ccagagaagc agaattgaag caatgctcca taaaagatta ttccactgca tccactgtgg
    25441 ataaatggca acggccagac cccacagacc aaatattaac gcgcgatcgc ttttgccaaa
    25501 tggaccggca taactgcgaa caccgttaat cgtctgcgcg agtaaaccgc aaaactcggt
    25561 caatatggtg caaaataaca tgagtatcac gagtgatgcg ttactttccg gtaaaaataa
    25621 aaatggtaaa tagagcgcaa tatcggaaat aacatcacca gtctcattca aaattgcccc
    25681 aagccgtgtt tgctgattgc actcacgcgc caacatgcca tccagcgcat tgagcgccat
    25741 acggataaaa agcacgatgg gcaatagcat aaagaggatg ggttgtgcca ccaacatcaa
    25801 cagcaatccg gtaagaagag aaagcgccag tgcagcaaga gtgatgtgat tcgctgtaac
    25861 gtggtgcttg taaagccaaa acatcgtcgg ccttaacagc gactgaaaga gcggttttat
    25921 ctggtagagc gtcattatca atccttgata attcggtttg cgctatggtg catcaattcc
    25981 cctgacggag atatcgcaac acgccgcttc attaaaaatt cagaatatat caaggtattg
    26041 gggactcact gtcaggaata attatcccca attaatcaaa gggttaagaa atattacgtt
    26101 gtggatcaat acaactcatc aagttttctc atcgcttgtt cccgtattgc gcgcgtctta
    26161 ccgttaagcg ccagctggcg gactaaatcc agttcagggt taacaagctc taacgccagc
    26221 aaaggatcac gtttcagcct cgcctttaat acatcaactg gaaaacgggg attattcatc
    26281 gccacaatcc accactcggg atcaatttct gccgccagta ctgcaggcgg tgtgtggcgg
    26341 ttgttaagtg cgccgaactt gaaaaatgtg agattgcttt tcgccagtgt actccaccat
    26401 atacgatccg gttgttttgc cagtacatcc agttgtgcta acgcatcggg gtcgagtgca
    26461 taatagacgc gtaatgcttc atcaacctgc gcacgggagc tgttttcctg cctcagaaaa
    26521 aacttcgccc aaatatcatt gcgtggcgta ttttgctgta attccttgag gacaacagcg
    26581 ggtaaattat cccaactaac taatgcactc tctaaaaagt cccagtcttc caaacgtatc
    26641 agggcggtgg catttaatag catctgatca tcaacttcac cgtggctgat aagctcagac
    26701 aaaatcatat cagcgctggt ttcatacgca gctgaatctg ctttcggatt atttttccga
    26761 tgcgataaag cacgttgcca aattttttgt ttcatcgacg tgctcagata ctgatttttc
    26821 gccagattac tccacccctc aggatcgttg aaacgtgtca ataaccgttg tagagcagcg
    26881 tcagagtgaa ttacctgcat atcccgacag caaaagtagg caggggattt tttctccatc
    26941 cttttaacaa aattaaactg taagttatct ggcaatgcta aaaataacac acgtattaga
    27001 tcatcgtcat tctcgaattt ttggtataaa taggctgcaa ctttgtcact ctcttgtggc
    27061 gtcatccgca ataattgacc atggtgtttc atctctgcca gcttgaacgc taagcttgtg
    27121 acaacattgt ttgatgaatc gttcgccaac tgcattgcct gattgtaggt gagttgtcct
    27181 ccagcaccga cctgacgaat agaggaatca tcactgcgga tatagccatc aataatggat
    27241 tcaggcaata accacgcatc tttataaagc tcctgaatga tcatatccca atctcttttc
    27301 gccagagtca ttacgatatc cagagggaga ttttcacgta gcgcaagatt caacggaccg
    27361 acaccatcag gtagacgact cattcgcgtc attatttcga cagagagcgg ttcacggtcg
    27421 agcaacccca gaacaacagg ctggtaaccc aattgctgat cggtcaactc cagaatctca
    27481 ttgtcaggca tatatctcgc catactttca atcgtttgtt catcattgct attaataagt
    27541 tgtttgcgta ggttagctga aattccttta agccttggga gagttcgcat tatggcaaga
    27601 tcaaatgtcg caatatctcg tccttcaaat gcagcaacgt tgccttcatc cagcatttgc
    27661 aattcctgca ctaactcagg agaaggctta gggcgaaaca gcgcttcttt tttcatctga
    27721 ttacagacat tccctgccat gcgaatattg cttcgcttaa aaatttcttt cagagcatct
    27781 tcatcattta ttagacccca ataatatgca tcaggattta ttatcatcag cgcttttttg
    27841 acattttcag tgttattttt gtcactcgca cggaccatga attcactatt tgtcgaagac
    27901 gcaacaacag gtgcaattat gtcattgata aatattccac gcgcattaat actcccctta
    27961 atcgaagacg gattatcgta atatgagtcg ataaatacca gtccacttgc atttatcgaa
    28021 ccggtaatta acagtggtga atccgtaact atattcgttg ccgtcacgtt accgagaata
    28081 acccatggat tagaaagaga gaaagtggaa atattacctt tgactgtcaa attaccaaga
    28141 acaatcaatt tttgattact ctctatatta ccttctacca ctaaatcgcc acgatgcact
    28201 ttccagtccg atgcagataa accgaagggg acaagattca cgctactgtt gatagcttgt
    28261 tcaacttgtt gtttatcttc agcggtagcc gcagaaaaaa atggttcaat atcaactgta
    28321 tttgcaacac atcgaagttc cgcctgtact ggaagactca gcataaacag gggaagtaaa
    28381 cctatccagc cgcaaatcct ggacttaaac atatgcactc catgccatta aaaaagaaac
    28441 aaaagggaaa tgcatctttc ccttttgtat attcgttatt ctcgcgaaat accatccaac
    28501 gtagacaaca cttcctcaga aagatgcaat ttttcagcag ccatattctc ccgtaaatgc
    28561 gcaacagaag acgtccctgg gatcagcaaa atattcggtg aacgttgtaa cagccacgcc
    28621 agcgccacct gcatgggtgt tgcacccagg ctcgcagcaa catccgacag cgtagaggat
    28681 tgcagcggtg taaagccccc gagcgggaag aacggcacgt aggcaatgcc atcgtgggcc
    28741 aaagcatcaa tcattgcatc atcagcacgg tgcgcgatgt tgtattcgtt ttgcacacag
    28801 acaatttcgg caatcttgcg cgcctctgca acctgcgtcg gtgtgacgtt gctcaggcca
    28861 atatgtttta ccaggccttg ttgttgcatc tctgccagca cggtcaggct ggcctcaatc
    28921 gatccttccg ctgggccatg accatctccc atcataacgc gcaggttaac cacatccagt
    28981 acgtccaggc cgagattacg tagattatcg tgcaccgctt tttgcaactc tgccggagaa
    29041 aaggcgggca accaggaagc gtcctctcca cgccgcgcac caattttagt gacaattgtc
    29101 aggtcgtcag agtaaggata aagcgcttcg cggataatct gattggtgac gtgcggacca
    29161 taaaagtcgc tggtatcaat gtgattgacg cccaatgcca gcgcctcgcg cagcacagtt
    29221 atagcgacgt ggcgatctct tgggggacca aaaaccccag gacctgccag ttgcatcgcg
    29281 ccataaccaa gacggttaac ggattttgta ccgagagtaa atgtattgct gctcatcgca
    29341 ccctgccctg taataaaacg gattaagaaa aatcagtata gaaacgaaaa gagataaata
    29401 atgcgagggc gtaaacaaga ttactggatg agttgctcta ttgtcctgaa aaacacaaaa
    29461 caaaacgcgt aaagcgccgc atccggcaat aaaaaaaccg caacctgtat cagcgttgcg
    29521 gtttaggtat cacatgctaa aaattagaat gttcagtatc tggacgttac tgtttcggtc
    29581 gcagattctc atcaaatacc agacgtttcc tgttcggttc aacttcccgc aatggctcaa
    29641 cttgatgcaa cgtctgctta caacgagtca ccagcacctg atactcatgc gtaccctgct
    29701 ttttccatgc cagctcctgc tcactgagtt cacgaatcgc tttcgccggg ctgccgacaa
    29761 tcaggtaatt ggcaggcatt tctgctttgg ctttcacaaa tgcggatgcg ccaacaatgc
    29821 tgttctcgcc aatcaccgcg ccgtccatca ctaccgcgtt cattcccact aatgcattgc
    29881 ggcggataat gcaaccatga aggatagcgc tatgaccaat atgtccatct tcttccacga
    29941 cagtatcctg ctcgggaaaa ccgtgcataa cgcaattatc ctgaatgttc gcgccatctt
    30001 tcaccacgat acgaccaaaa tcgccacgca ggctggcatt tggcccaacg taaacgccct
    30061 tgcctagaat aacatcgccg atcaataccg ctgtcggatg gacaaaactc tcttctggca
    30121 caaccggagt cagaccatct atctggtaaa ttggcatatc gcctcctgtt caatattcaa
    30181 accgccaaaa cgttgaaaat acaggcttcc cggcgcaggc agttcaccga ccgaggtttc
    30241 acctttttca ctaacgaacg ctaaagcagc aggcgctacg cgctgataaa tgttgataca
    30301 cagctgacgc gccgtatgcc ctgcccagtg cgccggaagc aactcctctg gcaacaatgg
    30361 gtctttaagg acgacacggc gataaaaatg gatcagtaaa agctgaatat gaaatgcacg
    30421 ctccggggtt aactcgtctg ctgcctcttt taacagcggc accagcgggc ggaatgactg
    30481 aataaaggtt tcgtacatgg cattttgttc agttaaatgc cagcactctt ccactctggc
    30541 acgcagtgct gcacgagaaa gcgccagtgg tatttgcgct tcaaaacaaa tcacgttatc
    30601 cgccaccccc gcttcatgca aaagtgtctg tacatccgcc agtttttgcg acggcgatgc
    30661 catcaggctg ggtgccagtg cgccaaaacc ttgccagatc aactgctttt tgacatcagc
    30721 cagcgttgat ttatctaaac cttccgagag caacaggagc catttaccat cccatgcagg
    30781 ttgctctgcg cgataaattt tactttctgc ccgtcgcgtc aggcgcaagc ctttatcact
    30841 gaggctatag aaactgcgtc gcccgatgcg ggaaacatcc agccagcctt ctttattaag
    30901 acgaaacaaa gcggtgcgca cgaaacgctc accaaatccc agcccttcca gcaaagcagc
    30961 cagactaccc agccagattt caccaccacg atgggaaagc gaatcaccat acagagagga
    31021 gatcaaagat gtgccactga ccggaacagc gtttacagca tgttggataa aagtaacaag
    31081 tttactcatt cgattcagtc tgttgttatt gttgtgattt atgaatcata gcacaggtcg
    31141 tatcgacttc tggccctggc gctttacctc accagggcca gacgacacgt caggcaccaa
    31201 caatattgcg cagatcaaac acccgacacg ccttgccttc tgaacgcggg atactgccac
    31261 agttaacgat catcacatcg gtagagatcc ccaccatcga cttaatccga tggcgcagct
    31321 ggtggcacac ctggcaacgc tgctcatgcg tcaatgtcag gctgctttct ttcaactcca
    31381 ctttcacaga aagtgaatca agatgccccc ggcggttcac ctccagttgg taatggggcg
    31441 ataaatgttc gaatttgaca atctcttctt ccagttgtga cgggaagaca tttacaccgc
    31501 gaatgatcaa catgtcatcg ctgcgtccgc tgatgcgatc catgcggcgc atagtccgcg
    31561 ccgttcctgg taacagtcgg gttaaatcac gcgtgcggta acgaatgacc ggcaatgctt
    31621 ctttggtcag cgtggtgaat aacagttcgc catgttcacc atcggcaagc ggtgtgccgt
    31681 catgaggatt aacaatttca ggatagaaat gatcttccca aatggttggg ccgtcggtag
    31741 tttccagaca ctccatcgcc ccccccggcc ccatcacttc tgacaggcca taaatatcca
    31801 gtgcggtgat ccccagacgc cgttcaatct ctttacgcat ggcctgtgtc cacggctcag
    31861 caccaaacac tccaacccgc agcgagcaac cgctggcatc accgcccaac tgccgctcca
    31921 gctcttcaat caggttaagg caataagatg gcgtaaccat gatcatatct ggttgaaaat
    31981 cgcggatcag ttgcgcttgt ttttccgtct gcccaccaga catcgggatc accgtagcgc
    32041 ccagacgttc ggcaccatag tgcgcgccca gcccaccagt aaacaggccg taaccatagg
    32101 caacatgaat tttgtctttg ggcgtgccac ctgccgcacg caaggaacgc gctacaatat
    32161 tggcccagtt atcaatatcg ttttgcgtat agccgacaac tgtcggttta cctgtggttc
    32221 ccgaagaagc atgaatgcgc accacttgtt ccatcggcac ggcaaaggtg tcgaagggat
    32281 agttgtcgcg cagatcctgt ttggtggtac agggaaattt acgcagatct gaaagttccc
    32341 tgaaatcatc aggatgtacg cctgctgcgt caaatttgcg ccgatacatc gggacatttt
    32401 catacgcgtg tttgagcgtc catttcagac gttgtgtttg caacgcctgt aactcatcaa
    32461 cagacgcggt ttcaatcggg tcaagctttg tattggttat cattgtaggg tactcgcagg
    32521 ttgatatgct caaacacgct ccagaatcat ggcgatgccc tgaccgacac cgatgcacat
    32581 ggtgcacaat gcgtaacgac cgttacgccg atgcagctca tggctggcag ccagtgccag
    32641 gcgggcacca ctcattccca acggatggcc taaggcgata gcgcctccgt tggggttaac
    32701 atgtggggca tcatcaggca gccccaattc gcgtagtaca cccaacgcct gggccgcgaa
    32761 cgcttcgttc agttcaatca cgtccatatc gtgaatactc agccccgcgc gttccagcac
    32821 ccggcgagtt gcaggcaccg gaccaagccc catcaggcgc ggttccaccc cggcggttgc
    32881 catggctacg attcgcgccc gcggtgtcag tccttgcgct gctgccatct gttcactggc
    32941 aataatcaac gcagcggctc cgtcattcac cccggaggca ttgcctgcgg taatcacccc
    33001 attggcacga aatggtgctt ttaacccacg taactgttcc agcgtcgttt ccgggcgcag
    33061 atgctcatca tgttgtattt ctgttacaac gcctttcttg tttttcaaca caaccggaac
    33121 aatctcctca gccagaatgc ctgaggattg cgcttttgcc gtacgttgct gactgcgtag
    33181 cgcaaaacta tcttgatctt ctcgcgatat ttttaacagt tcagctacat tctctgccgt
    33241 ttccggcatg ctgtcagttc caaattgttg agccatgagc gggttcacaa atcgccagcc
    33301 aatagtggta tcaaacatct cagcctgacg agaaaatgca ctggttgcct tgcccataac
    33361 aaacggtgcc cgagacattg actccacgcc accagcgatc agcaaatcgc catcgcccgc
    33421 tttaatcgcc cgtgcggcga accccagtgc gtccagcccg gaaccacaca agcggttaat
    33481 ggttgtgccg gaaacactat gcggcagccc cgccagtaac gtcgccatcc gggctacgtt
    33541 acggttgtct tctcccgcct gattagcaca gccgaggatc acatcatcga tacactccgc
    33601 atcgagacgc gggtttcgca ccagcagctc ccgcaaaggg atagcagcca gatcgtcagc
    33661 ccgaacacct gataatgccc caccatagcg gccaattggc gtacgaattc cgtcacagat
    33721 aaaggcttca cgcatcaggc ttctcctgta atggtgccgc cgatgcggtg agatttaccg
    33781 cgaaacagcg caaccgtttt ttgttgttgg ttaacaattt cgatgtcgta aacaccggtt
    33841 tgcttgccct gatgacgtac ctgcgcagta gcagttaagg tgtctccggc aaagcctgga
    33901 cgcaaaaaat caatcgtgca ggcagaagcg acggctgcca gcccctggct attgcaggcg
    33961 taggcaaagg cagtatcagc cagtgaaaat agctgcccgc cgtggcaact ttgatgaccg
    34021 ttaagcattt gtgcagtgac ggtcatggtc actacagcaa agccttcatc cattgagata
    34081 atgtcgatgc caagcgcttt ggcgcaggca tcgttctcat acattgcatg ggcattttgc
    34141 caggccttat gactcataac cgctctccag aagcgctcgt tggcgcagca atgaacatgg
    34201 gcgatagcgt tcttcgccat agtgatgttg tagattttcc aggagcctta atattcgctg
    34261 ccagccaagt tgcgctcccc aggcaagcgg gccatatgga taattcaccc caagacgcat
    34321 ggcggtatcg atatcctgtt cagaggccac gcctttttgc agcgcatcaa gggcttcatt
    34381 gatgatcatt gctaccgttc gccaaatcag cattcctggg taatctgcaa tttgcagcac
    34441 tgttttgccc tgctgttgca ggtaataaat ggccttgcgg gtcgctgagt ccgggttcac
    34501 cgctgccgca gcaatggtca ccaccttgcc cgccattttg tcgaccacta ccaccgggcg
    34561 tgccagtcgt attgccagcg cctgtgccgt ctcgccttgt gtctcaatca ataaaacatc
    34621 gtcaatttcc gtgacgccgt cacttttctt ttctactttc attgggctaa aactgtcgct
    34681 taccgcttcc aggccaacaa ctgcctcacg ttccgcgcgc cagtcgtaca cgcccagccc
    34741 acttttcttg cccaaccgtc caccaatcac cagttcctgt tgcaccagcg aaggtaaaaa
    34801 acgacgctcc tgccagaaag cgttaaacac cgaacaggtg acagcaaaat tgacgtcctg
    34861 accaatcaga tcggttaatt ccagcggccc catcgggaaa ccagcgccat cgcgaagtgc
    34921 agcgtcaatc acttctggtg cagcaacctg ctcttccagt gcccgccagg cctcggaata
    34981 ataaggacgc gccacacggt taacgataaa tccaggggtc gaatgacagc gcacaggctg
    35041 cttaccccaa ctcaacgtta gttcacacaa ctgctcaaca acttccgctg ccgttgccag
    35101 cccactgacc acctccacca acttcatcac cggtgccggg ttaaaaaaat gcagccccgc
    35161 aacacgttca ggatttttta cctccgcagc aatcgcggtt atagagattg acgaagtgtt
    35221 agtggtcaat agcgtttgtg gcgggcaaac ttccgccagc tgtgcaaaga gcgctttttt
    35281 gacttccaga cgttcagacg ccgcttcaat gaccaggtcc gcagctgcca gcgcgtgaat
    35341 atcggtcacc gggatcaggc gtttcaatgt gcgttcacag gtttcagcag tcagttttcc
    35401 ccgcgtcacg cgtgaattta gccgcgcgtg tatcccgtcg attgcgcggg tcagcgcttc
    35461 agcagaaatg tcatacagta aaacctgatg tccatgactg gcggcaactt cagcaatgcc
    35521 agcccccatg gtgccgctcc caatcactgc cacagtttgc acatttatca tcatgctatt
    35581 tccccgtgaa ctgcggtgag cgtttagcca ggaacgcact gacaccttca cgataatctg
    35641 cgctccgtcc ggcaagtcgc tgatagtcgc gttccagatc cagttgcgta tcgagcgtat
    35701 tggtttcagc gctatttatc gcttgcttga taagtccaag accaaatgtc ggttgtgtcg
    35761 ccagatgccg tgccagctgt tgcgcggtat ctgccagcgt ttcatcatca acaacctgcc
    35821 agatcatccc ccattcgtgc gcctgttcag cactcagttg attccccagc agtgccagcc
    35881 ccatggcgcg cgctcgccca gcaacgcgtg gcagtaacca ggttccaccg caatcgggta
    35941 ttaagcctaa cttactaaac gccatgacga attttgctga acgggcagca ataacgatgt
    36001 cgcaccccag tgccagtgtt gcgcctgccc ctgccgccac gccattgact gcacagatca
    36061 ccggttttgg cagttttgcc aggcgacgta ccagtgggtt atagaaacgt tcaactgaca
    36121 tccctaaatc cggtgcgggg cctgtgggat caacgttacg atcgttaaga tcctgaccag
    36181 cacaaaatcc gcgcccggca cctgtaagta acaggcaacg gatagtgtcg tcgcgctcga
    36241 cctgtttcag gcactctgcc agttgtgcgt gcatctcatc attaaaactg ttcaggcgtt
    36301 ccgggcggtt gagcgttagt gtcatcacgc ccttttctac atgactgagg atgaattcca
    36361 tcattagcgt cctttaaagt cgggcgtgcg tttttgtaag aaagcggaga tgccttcatg
    36421 acgatcttct gttgccgcca gcaaggtgaa taactgtcgc tcctgggcaa gtccggcttg
    36481 caaagccact tcctgcgact ggcgcagcgc ttgctttgcc gcttgtaagg ccaacggcga
    36541 gtgacgtgcc attttcgatg ccagctgtaa ggcgtattcg agggttaaat cgctggggaa
    36601 gacgtcgcta accagcccgg cctgctgtgc ttgctgggcg gtgatacttt ctccgctcag
    36661 caccattttg ctggctaacg atttaccgac actacggatt aaacgttgcg ttccgcctgc
    36721 gccaggcatg atgccgagag tgatttccgg caacccaaaa cgcgcgttct ctccggcaac
    36781 caccacatcg cacaacaatg ccagttcgca acccgcccca agcgcgtaac cattgacggc
    36841 tgcgataagt ggtttattaa aggcctgcaa tcgcgcccat agctgcggac gtgtatcgtt
    36901 taaggtggcc gcgagatctt tttctgccat ttcgttgaga tcggccccag cggcaaaaaa
    36961 gcgtgcatta ccggtaatca cacagaccga aatgctggta tcggtagccg cagcttccag
    37021 ttcatttacc agttgcatca gcagggcatt atttagcgca ttacgtgcgg cgggacggtt
    37081 aagggtcagc aacaatactc gttgctgacg gctgacgatc agttcgctca tgccatcccc
    37141 ttcgcgtcaa agtcaaccac cacatcgctg gtcagcggca gtgcctggca actcaacaca
    37201 taacctgcgg ccagttcatc cggttccaga ctgtaattgg tttccatcgc cactttgcca
    37261 cgcagcactt tgcatttgca ggtcgcacag acgccgcctt tgcaggcata gggcagatca
    37321 gccccctggc gcaatgccgc atcgagaatg ctttcatcgt cggcattaag cacgatttcc
    37381 cgatcccgcc catcctgacg tacagtcact ttttgtccgt cactttgcac gttaacgcta
    37441 cgtttgacgc gcgtgccagg cgtattaaac cgctccagat gaatggtttt atctggcatt
    37501 cccagtgctt ttaaggcggc ttccgtctca tccatcatcg ccgccggacc acaaataaat
    37561 gcctcatcat aaagacgaaa attaatgagc gaggccccaa gtgactgtaa tctttcaccg
    37621 tcaatacgcc cgtgaagcag atcgctgtcg agggtttcct gactgaaaat gcacaacaac
    37681 tgtaaacgct gaggatattt gtctttcagg tctgccagtg cctggcgaaa catcatgctc
    37741 tggctggtac ggttaccgta gatcagggtg aactgacttt caggctcggt ttgtaaagtg
    37801 gtggcgataa tcgccagcat tggcgtaata cctgatcctg ctgcaatagc cagatagcgg
    37861 ccctggcgtt cggcctgggg ctgatagccg aaatgcccct gcggcaccat gacctccagc
    37921 gtcataccct ggcggatgtg ttcgcgggca tagcgggaga aacgtccgcc ttcaatggct
    37981 ttcaccgcca cactaatttc gccaggcagg tagctgcggc aaatggagta acaacggcgt
    38041 aattcttcac catcaagact ggcttttaag gtcaaatgtt gaccggggcg aaagcgatac
    38101 gcctcctgca aaggctgggg caccgcaaag gtaatggtca ccgcatcacg ggtttccggc
    38161 tcgacttttg ccactgttaa ggaatgaaac gttgtcatgg catcctcaaa tacatttgaa
    38221 ataatcgaaa ggttcgcggc aactatcgca gcggtacaat gctttgcagg ccgtggaacc
    38281 aaattcactg ataagtgtgg tatggacgct ggcgcagcgc gggcaacgta cttctggcgg
    38341 caaatgggca tggcaactgt gtccggcggg cgggctaatg ccatactgtc gcagacgctc
    38401 acgggcatcg ggggtcatcc agtcggtggt ccatgccggg tcgagttgca gcacaacctg
    38461 aacgggggta aacccgtggg ttgtcattgc ctcacgtatc gcgccaatca aatgttccgt
    38521 tgccggacaa ccggaataag tcggcgtaaa gccgatcacc catccttctc ccatctgtgt
    38581 cacattacgc accatgccta aatcggtaat ggtcagcacc gggatctccg ggtccgggat
    38641 ctggctgagt agcgcccata tctcatgaac ctgtggcggt gcaatggtgg ccagacgttg
    38701 catacccatc tcctctgtta ccattgctga ccgggcaaga cacgctggag atactgcatt
    38761 tctgccagca tgggtccaag gtgttcggta tgcagtcctt ttttaccgcc agtgcgatac
    38821 gcctgctctt gcggtacgtt caatgtggct tcgttgattc cggcaaaaac ttcggcttcc
    38881 cacgctgcgc gtaaagtgcg tggatcaacc gcaataccct cttcactcag tgcaatatca
    38941 atctcgtcgg catcgaacag ttcggcagta aaacgccaca acttgtcgat ggcctgctgc
    39001 atcttttgcc ctgatacgtc aataccattg cccagtcgct ccagccagcc acgactaaaa
    39061 cgcaggtgat aacgcgcttc tttaattgct ttggcagaaa tcgccgccag ttgcggatca
    39121 cggctttcca tcagacgggt aaagagcgcc acatgccatg catcgatgaa atactggcgt
    39181 gcaatggtgt cggcaaaatt gccgtttggt tgttcaacca acaataagtt gctgaactgg
    39241 cgctcgtctc gggtgaaggc cagggtatct tcatcacctt ctcccgctaa ttcagcggca
    39301 tacgataaga agttacgtgc ctgacctaat aaatcgaggc caatgtttgc cagtgcgaga
    39361 tcgatttcca gttccggtgc gtgaccgcac cattccccca accgctggga gagcaccaga
    39421 tagttatcgc ccaggcgcaa ggtgtaagcc gttaactgat tcatttccga cctcacatgt
    39481 gctcaatgcc atcagggatg gtgtaaaacg ttggatggcg atagaccttg ctttcagccg
    39541 gatcaaaaaa ttcaccgcgt tcttccggtt gcgaggcaac aatttcactc gccttcacca
    39601 cccaaattga acatccttcg ctacgacggg tgtaagcatc acgggcattt tccagtgcca
    39661 tccgctcatc ggcagcatgt aaactgccaa catggcggtg tgataagccc tgtttgccac
    39721 gcacgaaaac ttcgtataac ggccagtaaa cattactcat tttgaatctc cttatgcgac
    39781 cttacgggca agttgttttt gtgcatgggc cagcgctgct tcccgtaccc aggtaccttc
    39841 ttcccaggct ttacgtttgg cgtcgagcct ctcctgatta caaattccgc gaccgttaat
    39901 tacttcgtta aactcctgcc agtcgatctc accaaagcgg tagtgaccgc tttcagtgtc
    39961 aaaatgcaga tccgggtcag gaacggtcat accgagcatt tcaacctgtg gaacggtgtt
    40021 gtccacgaaa cgctggcgga gttcgtcgtt ggtgaaacgt ttgattttcc aggcgagact
    40081 tctggcgctg tttggcgagt tatcatcgtt tggcccgaac atcattaagg ctggccacca
    40141 gaaacggtta atggcgtctt gcaacatctg cttttgagct tcgctgccct gcgccagtgc
    40201 catgcaggct tcaaacccct gacgctggtg aaaactctct tctttgcaga ttttcaccat
    40261 tgctctggca tacgggccat aagaagttcg gcataacgcc acctgattga cgatcgctgc
    40321 gccatccacc agccagccga taacgccgat atcagcccaa ctcaatgtcg gataattaaa
    40381 gatggaggaa tatttcatcc gtccgtcgag cattttttgg tagatgtctt ctctggcaca
    40441 gcccagtgtt tcggcggcgc tatagagata cagaccatga ccggcttcat cctgcacttt
    40501 ggctaacaga atggctttac gccgcaaggt tggtgcgcga gtgatccagt taccttcagg
    40561 caacatacca acaatctcgg agtgcgcatg ctgcccaatc tggcggatca atgtctttcg
    40621 gtaagcatcg ggcatccagt cctgcggctc gatagccgtt tcctgggcta tccgttgctc
    40681 aaagcgttct tcttgggtca cgtcatcacc tttacgattc cagttttcgt gaattaatta
    40741 aaaacacgaa tcacgttgtt ttatgaaagt tacacaatgg ttaactaaag tcacaaattt
    40801 tgtatacttg aactgtgaca gatttcgcac tctattttga taactcatta tttatcagtg
    40861 cattaatgtt tttagtagtt gatatacaga tcacgcagtt aacatttcgt taaaagatcc
    40921 cttgcttttt atgattcgcg atttaactat tagcaacaga aatgtgaaac atctggagag
    40981 tagcgatgca gcagttagcc agtttcttat ccggtacctg gcagtctggc cggggccgta
    41041 gccgtttgat tcaccacgct attagcggcg aggcgttatg ggaagtgacc agtgaaggtc
    41101 ttgatatggc ggctgcccgc cagtttgcca ttgaaaaagg tgcccccgcc ctccgcgcga
    41161 tgacctttat cgaacgtgcg gcgatgctta aagcggtcgc taaacatctg ctgagtgaaa
    41221 aagagcgttt ctatgctctt tctgcgcaaa caggcgcaac gcgggcagac agttgggttg
    41281 atattgaagg tggcattggg acgttattta cttacgccag cctcggtagc cgggagctgc
    41341 ctgacgatac gctgtggccg gaagatgaat tgatcccctt atcgaaagaa ggtggatttg
    41401 ccgcgcgcca tgtactgacc tcgaagtcag gcgtggcagt gcatattaac gcctttaact
    41461 tcccctgctg gggaatgctg gaaaagctgg caccaacgtg gctgggcgga atgccagcca
    41521 tcatcaaacc agctaccgcg acggcccaac tgactcaggc gatggtgaaa tcaattgtcg
    41581 atagtggtct tgttcccgaa ggcgcaatta gtctgatctg cggtagtgct ggcgacttgt
    41641 tggatcatct ggacagccag gatgtggtga ctttcacggg gtcagcggcg accggacaga
    41701 tgctgcgagt tcagccaaat atcgtcgcca aatctatccc cttcactatg gaagctgatt
    41761 ccctgaactg ctgcgtactg ggcgaagatg tcaccccgga tcaaccggag tttgcgctgt
    41821 ttattcgtga agttgtgcgt gagatgacca caaaagccgg gcaaaaatgt acggcaatcc
    41881 ggcggattat tgtgccgcag gcattggtta atgctgtcag tgatgctctg gttgcgcgat
    41941 tacagaaagt cgtggtcggt gatcctgctc aggaaggcgt gaaaatgggc gcactggtaa
    42001 atgctgagca gcgtgccgat gtgcaggaaa aagtgaacat attgctggct gcaggatgcg
    42061 agattcgcct cggtggtcag gcggatttat ctgctgcggg tgccttcttc ccgccaacct
    42121 tattgtactg tccgcagccg gatgaaacac cggcggtaca tgcaacagaa gcctttggcc
    42181 ctgtcgcaac gctaatgcca gcacaaaacc agcaacatgc tctgcaactg gcttgtgcag
    42241 gcggcggtag ccttgcggga acgctggtga cggctgatcc gcaaattgcg cgtcagttta
    42301 ttgccgacgc ggcacgtacg catgggcgaa ttcagatcct caatgaagag tcggcaaaag
    42361 aatccaccgg gcatggctcc ccactgccac aactggtaca tggtgggcct ggtcgcgcag
    42421 gaggcggtga agaattaggt ggtttacgag cggtgaaaca ttacatgcag cgaaccgctg
    42481 ttcagggtag tccgacgatg cttgcagcta tcagtaaaca gtgggtgcgc ggtgcgaaag
    42541 tcgaagaaga tcgtattcat ccgttccgca aatattttga ggagctacaa ccaggcgaca
    42601 gcctgttgac tccccgccgc acaatgacag aggccgatat tgttaacttt gcttgcctca
    42661 gcggcgatca tttctatgca catatggata agattgctgc tgccgaatct attttcggtg
    42721 agcgggtggt gcatgggtat tttgtgcttt ctgcggctgc gggtctgttt gtcgatgccg
    42781 gtgttggtcc ggtcattgct aactacgggc tggaaagctt gcgttttatc gaacccgtaa
    42841 agccaggcga taccatccag gtgcgtctca cctgtaagcg caagacgctg aaaaaacagc
    42901 gtagcgcaga agaaaaacca acaggtgtgg tggaatgggc ggtagaggta ttcaatcagc
    42961 atcaaacccc ggtggcgctg tattcaattc tgacgctggt ggccaggcag cacggtgatt
    43021 ttgtcgatta atcggtgaat gaagggcaac ggccaatagt tgccctttta tttcactaag
    43081 ttttgtgacg ttgtcacatt atgcatgatg tgtacatcta ttttcagggc acccactgta
    43141 tgaaaagctg gcacacctgc caaacaacct ggcaggtgca ggcaatcccc tttgcatcag
    43201 tactgataat gtgaacctga ctaaaccgcc cacagagcgc ggttgctaac aagaacacaa
    43261 catctgacga ggttaataat gggaagcccc tctctgtatt ctgcccgtaa aacaaccctg
    43321 gcgttggcag tcgccttaag tttcgcctgg caagcgccgg tatttgccca cggtggtgaa
    43381 gcgcatatgg tgccaatgga tgaaacgctt aaagaatttg gtgccgatgt gcagtgggac
    43441 gactacgccc agatctttac cctgattaaa gatggcgcgt acgtgaaagt gaagcctggt
    43501 gcgcaaacag caattgttaa tggtcaacct ctggcactgc aagtgccggt agtgatgaaa
    43561 gacaataagg cctgggtttc tgacaccttt attaacgatg ttttccagtc cggtctggat
    43621 caaacctttc aggtagaaaa gcgccctcac ccacttaatg cgctaactgc ggacgaaatt
    43681 aaacaggccg ttgaaattgt taaagcttcc gcggacttca aacccaatac ccgttttact
    43741 gagatctccc tgctaccgcc agataaagaa gctgtctggg cgtttgcgct ggaaaacaaa
    43801 ccggttgacc agccacgcaa agccgacgtc attatgctcg acggcaaaca tatcatcgaa
    43861 gcggtggtgg atctgcaaaa caacaaactg ctctcctggc aacccattaa agacgcccac
    43921 ggtatggtgt tgctggatga tttcgccagt gtgcagaaca ttattaacaa cagtgaagaa
    43981 tttgccgctg ccgtgaagaa acgcggtatt actgatgcga aaaaagtgat taccacgccg
    44041 ctgaccgtag gttatttcga tggtaaagat ggcctgaaac aagatgcccg gttgctcaaa
    44101 gtcatcagct atcttgatgt cggtgatggc aactactggg cacatcccat cgaaaacctg
    44161 gtggcggtcg ttgatttaga acagaaaaaa atcgttaaga ttgaagaagg tccggtagtt
    44221 ccggtgccaa tgaccgcacg cccatttgat ggccgtgacc gcgttgctcc ggcagttaag
    44281 cctatgcaaa tcattgagcc tgaaggtaaa aattacacca ttactggcga tatgattcac
    44341 tggcggaact gggattttca cctcagcatg aactctcgcg tcgggccgat gatctccacc
    44401 gtgacttata acgacaatgg caccaaacgc aaagtcatgt acgaaggttc tctcggcggc
    44461 atgattgtgc cttacggtga tcctgatatt ggctggtact ttaaagcgta tctggactct
    44521 ggtgactacg gtatgggcac gctaacctca ccaattgctc gtggtaaaga tgccccgtct
    44581 aacgcagtgc tccttaatga aaccatcgcc gactacactg gcgtgccgat ggagatccct
    44641 cgcgctatcg cggtatttga acgttatgcc gggcctgagt ataagcatca ggaaatgggc
    44701 cagcccaacg tcagtaccga acgccgggag ttagtggtgc gctggatcag tacagtgggt
    44761 aactatgact acatttttga ctggatcttc catgaaaacg gcactattgg cattgatgcc
    44821 ggtgctacgg gcatcgaagc ggtgaaaggt gttaaagcga aaaccatgca cgatgagacg
    44881 gcgaaagatg acacgcgcta cggcacgctt atcgatcaca atatcgtggg tactacacac
    44941 caacatattt ataatttccg cctcgatctg gatgtagatg gcgagaataa cagcctggtg
    45001 gcgatggacc cagtggtaaa accgaatact gccggtggcc cacgcaccag taccatgcaa
    45061 gttaatcagt acaacatcgg caatgaacag gatgccgcgc agaagtttga tccgggcacg
    45121 attcgtctgt tgagtaaccc gaataaagag aaccgcatgg gcaatccagt ttcctatcaa
    45181 attattcctt atgcaggtgg tactcacccg gtagcaaaag gtgcccagtt cgcgccggac
    45241 gagtggatct atcatcgttt aagctttatg gacaagcagc tttgggtaac gcgttatcat
    45301 cctggcgagc gtttcccgga aggcaaatat ccgaaccgtt ctactcatga caccggtctt
    45361 ggacaataca gtaaagataa cgagtcgctg gacaacaccg acgccgttgt ctggatgacc
    45421 accggcacca cacatgtggc ccgcgccgaa gagtggccga ttatgccgac cgaatgggta
    45481 catactctgc ttaaaccatg gaacttcttt gacgaaacgc caacgctagg ggcgctgaag
    45541 aaagataagt gattgtttca gacaaaaaaa cggcaccagg tgccgttttt tacttatgag
    45601 cgaaccagat taataccgta cacacaccga cttagtttca caccaaccgt ccagccagtc
    45661 ggggccaaaa tcacggcccg ttcctgactg cttcatccca ccaaacggta agttagcgtc
    45721 aattaaggta tggctgttta cccacaccgt ccctgcctgt aagcgatcgc tatattccag
    45781 agcctgggag agattttgcg tccagacact ggcagttaag ccatattccg tgtcgtttgc
    45841 cagttgtaac gcctcttctc catccgctac tcgcaccagg tttaccaccg gaccaaacac
    45901 ctcttcacga gttaagcgca atttagcatc gggatttacc accagcgttg gcgcaacata
    45961 atacccctct ccggctggtc cattcgaccc gcgaatcagc tctgcttgct gtgcctgcgc
    46021 atcgtcgagg aatgaacaca ctttgtcgca gtgcgcacga gaaaccaaag ggttaatctg
    46081 tgcaacaggt gacatccccg gtcccacttg caacgatttt accgcctgct caaatccact
    46141 aaccagcgtg tcaaacaacg gcgcttcaat ataaattcgc gaactggcgg cgcatacttg
    46201 cccttgattc aggaagcttc cggtcatcaa gccttcaata acccattgcg gatcagcatc
    46261 ttttaataca attgccgggt ttttaccgcc cagttccagc gttacacgcg ttaagtgatc
    46321 agcagcagtt ctggcaatac cttttcccgt cgcggttgaa ccggtaaaac tgattttcgc
    46381 aacatgagga tgtgacgtca gggccgcgcc gcatacagca cctgacccgg tgacgacatt
    46441 aaaaacgcca tcagggatac cagcctcgct ggccagttcc gccacgcgca acatcgtcag
    46501 tggcgtggtt tccgaaggct taatcacgat tgaacagcct gctgccagtg ctggcatcac
    46561 cttccacata ccaatcatca acggaaagtt ccatggcaca attcccgcca ctacgccaac
    46621 cggctcttta cgcgtccagg cctgataacg cgccccctgg ggtaagggaa tcgacaagtc
    46681 cagcgtttta cccgcgattt tggtcgttaa cccggcggta taacgcatcc agttcagcgt
    46741 acagcccact tcaaaagcac gagaaatggc aattgacttg ccttgctcca gggtttccag
    46801 ttgcgccagc tcctcactgt gctgctccac cagatcagca aaacgtagca gaatacgttc
    46861 acgctctgcg ggtaatcgcc cggcccagcg acgcgagaca aaggcccgcc aggcagacat
    46921 gactgcgtta tctacatccg cttcgttggc atcagcagta gacgcaattt cttgcccggt
    46981 ggccggatca aagatcgcca atcgtttttc actttgtgcg gggcctggac gaccatcaat
    47041 ataaagaccg tgttgacgat cgagaaactg ttggacctgg cttaatactg ctacatgcgg
    47101 ctctgtcatc acttttcctt attatttacc cagtgtgatt tcagtgtacg cagcgacaag
    47161 aaaagtcact tttttcgctg tgacattcgc tttgtcagct gtgacaagct ccgcaaatcg
    47221 tgacaataac cgcttatgtc tctatgcctg agaagactta tttcctatag tcaggctcat
    47281 tagcttcgtt tactgcaaca caaatgcaac aataaaaata catttcacag agcgaaaacg
    47341 tgccatgaac cccgcagtgg ataatgagtt tcagcaatgg ctttcccaaa tcaatcaggt
    47401 atgcggaaat tttaccggac acctgctgac tgagcgttac acgggtgtac tggatacgca
    47461 ttttgccaaa ggactaaagc tgagtaccgt gacaaccagc ggggtgaatt tatcccgcac
    47521 ctggcaggaa gtaaaaggca gcgacgatgc ctggttttac accgtttttc agcttagtgg
    47581 tcaggcaata atggagcagg atgagcgtca ggtgcagatt ggcgctggcg atattacgtt
    47641 actcgatgcc tcacgcccct gttcgcttta ctggcaggag tcttctaaac agatttcatt
    47701 acttttgcca cgcactctgc tggaacaata ttttccccat caaaaacctg tctgcgcaga
    47761 aagactggac gctgacttac ccatggtgca actcagtcat cgcctgttac aggagagcat
    47821 gaataatccg gcactttctg aaacagaaag tgaagctgcg ctacaggcga tggtgtgtct
    47881 gctgcgcccg gtacttcatc agcgggaatc tgttcaacct cgtcgtgaac gtcagtttca
    47941 aaaagtggtt acgttgatag acgataatat tcgcgaagag atattacgcc cggagtggat
    48001 agccggagag acaggtatgt cagtacgtag tttgtaccga atgtttgccg ataaaggttt
    48061 ggtagtcgcg caatatattc gtaaccgtcg tctcgatttt tgtgcagatg cgattcgcca
    48121 tgccgcagat gatgaaaaac tggcaggcat cggctttcat tggggatttt ctgaccagag
    48181 tcatttttca acggtattta agcaacgctt tgggatgacg ccaggcgagt atcgacgtaa
    48241 attccgctaa ctaattggcg ttgtgtgtac tgcaacgcca attagttaaa acagatattt
    48301 gactatgcat ttttcagggc gaaaggcaat ttgacaggag tttttgaata ttcaccttgt
    48361 ttcaggaaaa tagatcaatc gctttctcct gtagcgttat cccgatattg cggttttgct
    48421 attaaaattt tcgcacccat ttaccgttta tcccttgtac atattccccc ggtttggccc
    48481 gagctacgag cttttgcccg gcgagtttcg cgatatcatc tacagacaca ttattttgct
    48541 ttgccagttg ttggtaactc gctttacgtt cggcattaat gtcggttact aatttctctg
    48601 tttcagcatc cgttttcagc gcaaccagat aaccataaaa tgtttcacct acccgcccct
    48661 gagttctggc ttcatccaga gtcaatgcca ttacattgct gcttaccagc ccaacaagaa
    48721 acgcacaaaa aagtaatgtt ttcttcatca tcgcctcaga aaagatcgct acgagtttca
    48781 agcagctctt cgacatcttt gtctgccttg atgatgatct catgctcaat tttaacgttc
    48841 atattgatag tgatcggttc cttgggcgca gcgacttcaa tacgcggcgt acaggcagcc
    48901 agcataaaag atgacgtcaa cgcagccagt aaaattttca ttgtttttcc tcacactctt
    48961 ttccttgcgg acagtcattt cccggcagac gtgcgttctg ctccaaccat gcctggagat
    49021 tatcgccaaa gcgtaaactg cgccacagcg taaaaatatt ctcttcatga tggtaattaa
    49081 ggtttaccgt accgctttta ccatcaacgc gactggtacc tgtaatgttg gcctgcatgg
    49141 ttaataaacc gagattatct aagttaattt ttgtcgatga ccggctaatt tccatatagc
    49201 gcaaccagtt aattgctgaa cccgcagtca tattgtcttt caccaccgca tctgcggtgt
    49261 ctttatccag ccgcaaggtc attggcccgc tatttgccag ccaaccatct ttcactatcc
    49321 atttttcgct gtttagccat aacggcaatg caccactaaa tgccccggac atggcgaatt
    49381 gtttcggatt gacggcgcta accagttcgc tggatgaaag attattcagc cgcaacagcg
    49441 ccgggtcatg ttgcggcata cgtaattgtt tcatcagtac gttaccgcct aacacatcga
    49501 cgctaacatc agtcaacagc aagggttctt cttcagtcca cggataacgc ccttgcaaat
    49561 cagccgtaat atttttcgct gtcaccagat taatcacttc ggcaattcgc aacgtaacgg
    49621 ggccgcgagt ccccagatgc caggctccat cggcaaaacg gaaaggcagg acaaaatcga
    49681 caccgttaac ctgattatct ggcatccagg cactaccgcc tttcaacacg ccgtgtcctc
    49741 ccgcgcggaa tccttgttca ggcgcagcag aaaatgcaac ctgagcatac agttcaccat
    49801 cgcgtaagtt catcttccag tcgggtggca ccagcggctg gaatacagtc agtgattgtt
    49861 ttggccacca ggcgttaccg cgcagacgaa taccgtccca gcgaccattt acccgaaccg
    49921 ggccaatctc accagcatgt aaatcgcctt taaagaggaa atacgtcgga tcgcgcccat
    49981 cgacgctaaa ttttaaggtt gatggcggta acacactgcc gccagtgaac agcgtttgcc
    50041 cggcgtccag tgacagcgcg ccgctgaagg agggatgctg tgcgtcacgt acccagacga
    50101 cgggcttgtc gagaattaat cgcggctttt ctaccgtcat cgtaccgtat tgtaactggt
    50161 cgaaaccggt ggagaggtcg gtcagcgtaa tcgtgctgtc atgccattca ccttttcctg
    50221 cgacatccca ggtggcattc atcggtgtaa aactgccttt tccccagtag cgccactgcc
    50281 agcggcccgc gtcagggaga aaatcattcg ccagcccatc catatgcagc acgaaatcgc
    50341 ccagttcatt ttcatgcgcc tgcaaaatgg cctgcaaacg tccgtcaaca ccacgttggg
    50401 tgacttttac acccgctaaa ggccagcgga tttcatcgat gtccagcgaa tcgatgactc
    50461 ttcccttcga acgtaataac gcgcctggct caaaggccag cgttgggtca gtcagacttc
    50521 cacttagctg cgcgggtaag cgggcatata aaatgaggtc cgcctgtttc gcttcaccgg
    50581 tcagttgcag aggcagctgg ctgttatcca tgcttaattt tcctgggcca aaatttagca
    50641 ccgcgttgcc tttacccgct tgcccctggg tcagcacact cagtcgtccg ctgatcagag
    50701 cgttctcaag ccctgcctgc cagttgtcga ctttgacacc cagtcggcca ctcaaaggaa
    50761 aacctgcata cggccaactc cagcgaccat cgcttacggt caattgttga cgagtaattt
    50821 gccacggcaa atcgagcaac ggatcgccgt tatcccgtgc cagcacaatc aattgcccgc
    50881 tattttcctg ccagtccagc tctgcatcca ccagtgacgg ttcctgcggc aagtttaacg
    50941 tcgcagtagc atgcccactt acaggaagtc catccggcac gagcggcata gtaaattccc
    51001 ccaccagttt aaccggcggc tgattttcaa acgcgacgac atccagttcg ctgactgtaa
    51061 gttgttgccc tttcagctgg ccttgaaatt taactttttc gccctgataa cgcagttgct
    51121 ggatatcaga ggttaatgcg agagagagtt ttccctgcca ttcctgccag ggagaaaaaa
    51181 tcagtttatc gatattgatc caggtgttag gcagcatgga ctgccactgc gcgagggttt
    51241 ttggagcagc tggcgattgc tccgtctgcg gcaatttcgc cagacaagca gaatcaagtt
    51301 ctaccgtgcc gacgttcaat aaccagcggc tgggatgtga aaggctggcg ttggtgatat
    51361 gcgcaagctg acaatctccc accagataac ggagatcggg aatgattaaa cctttacgcg
    51421 taatgcgtgg gctttcatct aatgcaatac gtgtcccgag cggtagccag atgcccgcca
    51481 gagtgggaac ccacagcccg agcgtcatca acagcgtcaa cggcacaaga ataatcagta
    51541 ataacagcgc gagaacggct ttatatttac ccagcatggg tagttaatat cctgatttag
    51601 cgaaaaatta agcattcaat acgggtattg tggcatgttt aaccgttcag ttgaaggttg
    51661 cgcctacact aagcatagtt gttgatgaat ttttcaatat cgccatagct ttcaattaaa
    51721 tttgaaattt tgtaaaatat ttttagtagc ttaaatgtga ttcaacatca ctggagaaag
    51781 tcttatgaaa ctcgccgttt atagcacaaa acagtacgac aagaagtacc tgcaacaggt
    51841 gaacgagtcc tttggctttg agctggaatt ttttgacttt ctgctgacgg aaaaaaccgc
    51901 taaaactgcc aatggctgcg aagcggtatg tattttcgta aacgatgacg gcagccgccc
    51961 ggtgctggaa gagctgaaaa agcacggcgt taaatatatc gccctgcgct gtgccggttt
    52021 caataacgtc gaccttgacg cggcaaaaga actggggctg aaagtagtcc gtgttccagc
    52081 ctatgatcca gaggccgttg ctgaacacgc catcggtatg atgatgacgc tgaaccgccg
    52141 tattcaccgc gcgtatcagc gtacccgtga cgctaacttc tctctggaag gtctgaccgg
    52201 atttactatg tatggcaaaa cggcaggcgt tatcggtacc ggtaaaatcg gtgtggcgat
    52261 gctgcgcatt ctgaaaggtt ttggtatgcg tctgctggcg ttcgatccgt atccaagtgc
    52321 agcggcgctg gaactcggtg tggagtatgt cgatctgcca accctgttct ctgaatcaga
    52381 cgttatctct ctgcactgcc cgctgacacc ggaaaactac catctgttga acgaagccgc
    52441 cttcgatcag atgaaaaatg gcgtgatgat cgtcaatacc agtcgcggtg cattgattga
    52501 ttctcaggca gcaattgaag cgctgaaaaa tcagaaaatt ggttcgttgg gtatggacgt
    52561 gtatgagaac gaacgcgatc tgttctttga agacaaatcc aacgacgtga tccaggatga
    52621 cgtgttccgt cgtctgtctg cctgccacaa cgtgctgttt accgggcacc aggcattcct
    52681 gacagcagaa gctctgacca gtatttctca gactacgctg caaaacttaa gcaatctgga
    52741 aaaaggcgaa acctgcccga acgaactggt ttaatcttgc cgctcccctg caatccaggg
    52801 gagctgattc agataatccc caatgacctt tcattctcta ttcttaaaat agtcctgagt
    52861 cagaaactgt aattgagaac cacaatgaag aaactagccg cgtttgttgc gctaagcctg
    52921 ctgatggcgg gatgtgtaag taatgacaaa attgctgtta cgccagaaca gctacagcat
    52981 catcgctttg tgctggaaag cgtaaacggt aagcccgtga ccagcgataa aaatccgcca
    53041 gaaatcagct ttggtgaaaa aatgatgatt tccggcagca tgtgtaaccg ctttagcggt
    53101 gaaggcaaac tgtctaatgg tgaactgaca gccaaagggc tggcaatgac ccgtatgatg
    53161 tgcgctaacc cgcagcttaa tgaactcgat aacaccatta gcgaaatgct gaaagaaggt
    53221 gcacaagtgg atctgaccgc gaaccagtta acgctggcga ccgcgaaaca gacattaact
    53281 tataagctgg cggatttaat gaattaatag ctgccacagc tcccggcggc aagtgactgt
    53341 tcgctacagc gtttgccgtt gggtaatgca cacatcccaa tcgccgtacc atccagttga
    53401 cgggcaacag aaagcgaacc gccgatcatt gcacaatttg cttctccact actggacatc
    53461 gacgctttta aacctggcgc tacgtgcgcc gcagtcgcct gctgaacagg ttcactacta
    53521 cacgccgaca acaataaagc ggcacaccct acccaaaacg ctgctcgcat ctcttcttcc
    53581 tctgatcttc aagccaaacg acaccgccat aaataatagg cagcacagag ggcggcgtcg
    53641 agagctgtcc tgcgcgttgc cccgcatttt tactttttta tggctatttt tttgccctct
    53701 gtttgatcaa aacattcatt acgctgatgt gggggacaca aaagcgaaaa tgcagaagaa
    53761 agccatttgc taaaattgaa ggattactac tgggcgcgca gcaatttcgt gcgcccctca
    53821 tttgcgcaat gtaagggtgt catatgatta ctattgacgg taatggcgcg gttgcttcgg
    53881 tggcgtttcg caccagtgaa gttatcgcca tctaccctat tacccccagt tccacgatgg
    53941 cagaacaggc tgatgcctgg gccggaaacg gcttgaagaa cgtttgggga gacacaccac
    54001 gcgtggttga aatgcagtcg gaagcgggtg ctatcgctac cgtgcatggc gctttgcaga
    54061 cgggtgccct ttcaacatcg tttacgtcat cgcagggttt gctgctgatg atcccgacgc
    54121 tgtacaaact ggcaggcgaa ctcacaccgt ttgtcctgca tgtagcggca cgtaccgttg
    54181 ccacacatgc actctctatt tttggcgatc attccgacgt catggcggtg cgccagacgg
    54241 gttgcgcgat gttgtgtgcg gcaaacgtcc aggaagcgca agactttgct ctcatttcgc
    54301 acatcgcgac gctgaaaagc cgcgtgccat ttattcattt ctttgatggt ttccgcacgt
    54361 cccacgaaat caacaaaatt gtcccgctgg ccgatgacac gattcttgat ctcatgccgc
    54421 aggccgaaat tgatgctcat cgcgcccggg cactcaaccc ggaacatccg gtgatccgcg
    54481 gtacgtccgc caatcctgac acttatttcc agtctcgcga agccaccaac ccatggtacg
    54541 acgcggtcta tgaccatgtt gaacaggcga tgaatgattt ctctgccgcg acaggtcgtc
    54601 agtatcagcc gtttgaatat tacgggcatc cgcaagcgga acgggtgatt atcctgatgg
    54661 gctctgccat tggcacctgt gaagaagtgg ttgatgaatt gctaacccgt ggcgaaaaag
    54721 tcggcgtgct gaaagttcgc ctgtaccgcc ccttctcagc caaacacttg ctacaaactc
    54781 taccgggatc cgtacgtagc gtggcggtac tggacagaac caaagaaccc ggtgcccagg
    54841 cagaaccgct ctatctggat gtgatgaccg cactggcaga agcctttaat aatggcaagc
    54901 gcgaaactct cccccgtgtc attggtgggc gctatggtct ttcatccaaa gaatttggcc
    54961 cggactgtgt actggcggta tttgccgagc tcaacgcggc taaaccgaaa gcgcgcttta
    55021 cggttggtat ttacgatgat gtaaccaatc tgtccctgcc gttgccggaa aacacccagc
    55081 caaactcggc gaaacttgaa gccttgtttt atggccttgg tagtgatggc agcgtttccg
    55141 cgaccaaaaa caatatcaag attatcggta attccacgcc gtggtacgca cagggctatt
    55201 ttgtttacga ctccaaaaag gcgggcggcc tgacggtttc tcaccttcga gtgagcgaac
    55261 agccgattcg ttccgcttat ctcatttccc aggctgattt tgttggctgc caccagttgc
    55321 agtttatcga taaatatcag atggctgagc gtttaaaacc tggcggcatt ttcctgctca
    55381 acacaccgta cagcgcagat gaagtgtggt cgcgcttgcc gcaagaagtt caggccgtgt
    55441 taaaccagaa aaaagcgcgc ttctatgtga ttaacgcggc gaaaatcgcc cgcgaatgtg
    55501 gcctggcggc ccgtattaat accgtcatgc agatggcttt tttccatctg acgcaaattc
    55561 tgcctggcga tagcgccctc gcagaattgc agggtgcgat tgccaaaagt tacagtagca
    55621 aaggccagga tctggtggaa cgcaattggc aggctctggc gctggcgcgt gaatccgtag
    55681 aagaagttcc gttgcaaccg gtaaatccgc acagcgccaa tcgaccgcca gtggtttccg
    55741 atgccgctcc tgatttcgtg aaaaccgtaa ccgctgcgat gctcgccggg cttggtgacg
    55801 ccctccccgt ttcggcgctg ccgccagacg gctcctggcc gatgggcact acgcgctggg
    55861 aaaaacgtaa tattgccgaa gagatcccca cctggaaaga ggaactctgt acccaatgta
    55921 accactgcgt tgccgcttgc ccacactcag ctattcgcgc aaaagtggtg ccgcctgaag
    55981 cgatggaaaa cgcccctgcc agcctgcatt cgctggatgt gaaatcgcgt gatatgcgcg
    56041 ggcagaaata tgtcttgcag gtggcaccgg aagattgcac cggttgtaac ctgtgcgtcg
    56101 aagtttgccc ggcgaaagac cgtcagaatc cagagattaa agccatcaat atgatgtctc
    56161 gcctggaaca tgtcgaagaa gagaaaatca attacgattt cttcctcaac ctgccagaaa
    56221 tcgaccgtag caaactggaa cgtattgata ttcgtacatc gcagctgatt acaccgctgt
    56281 ttgaatattc aggtgcttgc tccggttgtg gcgagacgcc gtatattaaa ttgctgactc
    56341 agctctatgg cgaccggatg ttgatcgcta acgccaccgg ctgttcttca atttatggcg
    56401 gtaacctgcc ctctacaccg tataccaccg atgccaacgg tcgtgggccg gcatgggcga
    56461 actctctatt tgaagataat gccgaatttg gccttggttt ccgcctgacg gtcgatcaac
    56521 accgtgtccg cgtgctgcgt ctgctggatc aatttgccga taaaatcccg gcggaattac
    56581 tgacggcgtt gaaatcagac gctacgccag aggttcgtcg agaacaggtc gcagctttac
    56641 gccagcaact caacgatgtt gccgaagcac atgaactgct gcgtgacgca gatgcactgg
    56701 tggaaaaatc aatctggctg attggtggcg acggctgggc ttacgatatc ggctttggcg
    56761 gtctggatca tgtattgagt ttgacggaaa acgtcaacat tctggtgctg gatacgcaat
    56821 gctattccaa caccggtggt caggcgtcga aagcgacacc gctgggtgca gtaactaagt
    56881 tcggcgagca cggcaaacgt aaagcgcgta aagatcttgg cgtcagtatg atgatgtacg
    56941 gtcatgttta tgtggcgcag atttctctcg gcgcgcagct gaaccagacg gtgaaagcga
    57001 ttcaggaagc ggaagcgtat ccggggccat cgctgatcat tgcttatagc ccgtgtgaag
    57061 agcatggtta cgatctggca ctcagccatg accagatgcg ccaactcaca gctaccggct
    57121 tctggccgct atatcgcttc gatcctcgtc gtgccgatga aggcaaactg ccgctggcgc
    57181 tggattcacg cccgccgtca gaagcactgg aagaaacgtt acttcacgag caacgtttcc
    57241 gtcgtctgaa ttcgcagcag ccagaagtgg cagaacagtt atggaaagat gctgcagctg
    57301 atttgcaaaa acgctatgac ttcctggcac aaatggccgg aaaagcggaa aaaagcaaca
    57361 ccgattaaat gctctggata aggattatcc aattctaaaa aaaaagcccg gacgactgtt
    57421 cgggcttgtc tttttatatg ttggaaaatc agtggcaatg caatggccca acagaaaata
    57481 aaatctgtga caataaaggc atataacccg cgcagaataa cgtatagaaa attaaaagta
    57541 taatttttat tttgtatgta ttatcttcga tacattcacc agacttatta tcatttcagg
    57601 agaattattt gattaaggtt ttacttaagg cgtaacaaat gatatttatc ggctaactga
    57661 acttctcctt taggatgttt tcactcccca aaatggggat aaaaggcaaa taaaataact
    57721 aaaggattta ttcaatgaaa agcaaagtac tggcactttt aattcctgcc ctgctcgccg
    57781 caggtgctgc acatgcagcc gaagtttata ataaagacgg caacaaatta gatctgtatg
    57841 gcaaagttga tggcctgcat tatttttctg ataattcagc gaaagatggc gaccagagct
    57901 atgcgcgtct gggttttaaa ggcgaaaccc aaattaacga tcaacttact ggctacggtc
    57961 aatgggaata caatattcag gcaaacaaca ctgaatcttc aaaaaaccag tcatggaccc
    58021 gtctggcatt tgccggactg aaatttgcag attacggttc tttcgattac ggacgtaatt
    58081 atggcgtaat gtacgacatc gaaggctgga ccgatatgct gcctgaattt ggcggtgact
    58141 cttataccaa tgcagacaac tttatgactg gtcgagccaa tggcgtcgcg acttatcgta
    58201 atactgattt cttcggtctg gtaaatggtc tgaacttcgc ggtgcagtat caaggtaaca
    58261 acgaaggtgc cagtaatggt caggaaggca ccaacaacgg acgtgatgtt cgccatgaaa
    58321 acggtgacgg ctggggtctt tccacaacat atgatttagg catgggcttt agcgctggtg
    58381 cggcatacac ctcttctgac cgcaccaatg accaggttaa ccatactgcg gcgggtggtg
    58441 ataaagcaga cgcgtggact gctgggctaa aatacgatgc taacaatatt tacctggcaa
    58501 ccatgtattc agaaacgcgt aatatgaccc cgtttggcga cagcgattat gctgttgcaa
    58561 acaaaaccca gaactttgaa gtcactgcac agtaccagtt tgattttggt ctgcgtccgg
    58621 cagtctcttt cctgatgtct aaaggccgtg acctgcacgc tgccggtggt gcagacaacc
    58681 cggcaggtgt tgatgataaa gatctggtta aatacgccga tgttggcgcg acttactatt
    58741 tcaataaaaa catgtccact tatgttgact ataaaatcaa cctgttggat gaagatgaca
    58801 gtttctacgc tgccaacggc atctctaccg atgatattgt cgctttaggt ctggtttatc
    58861 agttctaaat cctcctgccc gctgttatgg cgggcttttt ctgcttattc ttcctccttt
    58921 gatctaaatt aaaaatgtga actccgtcat tacacaaaaa gtgtcatctg gcgttacact
    58981 ttatgcggat actaaaacag gaggttttat gaacagaacg attcttgtcc ctatcgatat
    59041 ttccgattca gaattaactc aacgcgtgat tagccacgtt gaggcagagg caaagattga
    59101 tgatgcagag gttcatttcc tgacggtaat accttcactg ccctactatg cctctctggg
    59161 tttagcgtat tccgcagaat taccggcaat ggatgacctg aaagcggaag ccaaatcgca
    59221 actggaagag atcattaaaa aatttaaact gccaaccgac agagtgcatg tccatgttga
    59281 ggaaggctcg ccaaaagacc gcattctgga attggcgaag aagatccccg ctcatatgat
    59341 catcattgct tcccatcgac cggatatcac cacttatctg ctcggttcta acgccgcagc
    59401 tgtagtgcgt cacgcagagt gctccgtgct ggttgtgcgc tgacactaac gcccgcacat
    59461 tgctgcgggc tttttgattc atttcgcaaa tgtgctgaca ttttcccctc taatccgtac
    59521 catacacgcc acagttttta tatcagattt cttatgctgg gcgttccggc atgacgcata
    59581 ctcttctgat gccatataac gaattgagtc gcttttaaat gtcgcaaaat caagaaatta
    59641 gtaagaaaga acaatacaac ctgaacaaat tacaaaaacg tctgcgtcgt aacgtgggcg
    59701 aagccattgc tgacttcaat atgattgaag aaggcgatcg catcatggtt tgcctctccg
    59761 ggggtaaaga cagctatacc atgctggaga ttctgcgcaa tttgcagcaa agcgcgccaa
    59821 tcaatttttc gctggtggct gttaacctcg atcaaaagca accgggcttc ccggaacacg
    59881 ttctgcccga gtatcttgaa aagctgggcg ttgagtacaa gattgttgaa gagaatactt
    59941 acggtatcgt gaaagagaag attccagagg gcaaaaccac ttgctcactg tgttctcgcc
    60001 ttcgtcgcgg tatcctttat cgtaccgcaa cggaactggg ggcgacgaag atcgcgttgg
    60061 gtcaccatcg tgacgatatc ctgcaaacgt tgttcttaaa tatgttctac ggcggcaaga
    60121 tgaaaggtat gcctccgaaa ctgatgagcg atgatggcaa acatatcgtt attcgtccgc
    60181 tggcctactg ccgcgagaaa gatattcagc gatttgccga tgcaaaagcg ttcccgatta
    60241 ttccgtgcaa cctgtgcggt tcacagccta acctgcaacg tcaggtgatt gctgacatgt
    60301 tgcgtgactg ggataaacgt tatccagggc gtatcgagac gatgttcagc gcgatgcaga
    60361 atgtggtgcc gtcgcatctg tgcgatacca acctgttcga ttttaaaggc attacccacg
    60421 gttctgaagt ggttaacggg ggtgatctgg cgtttgatcg cgaagagatc ccactacaac
    60481 cggcgggctg gcagccagaa gaagatgaaa atcagttgga tgagttacga ctgaatgtgg
    60541 ttgaagtgaa ataaccagga tagcgcccga tgcgcaagcg tatcgggcta ctcttatgga
    60601 ggccggataa gacgcggcca gcgtcgcatc cggcaatccc gaataagatg tttactcttg
    60661 cacccggcaa ttcaacattt cattatttta ataaccgcac ccggcacgtt tttcctttaa
    60721 tcttcccgcc ctgtaactgt ttccatgctt tatgagcaac agcctgacgg accgcgacat
    60781 agacatgcgc cggatgcacg gcgattttgc caatatctgc gccatcaagc ccgatatctc
    60841 ctgtcagtgc acctaataca tcacccgggc gcattttggc ttttttcccg ccatcgatac
    60901 acaacgttgc catttctgct tccagcggca caatggaact attagctggc ggcgtttgcc
    60961 agttaagttt tatctgcaac atgtcagaaa tgatattggc ccgctgtgct tcttccggag
    61021 cacagaaact gatcgccaga ccgctatttc ctgcacgagc tgtacgaccg atgcgatgta
    61081 catgaacttc agggtcccac gccagctcaa agttcaccac cagctcaagc gatttaatat
    61141 ccagaccacg cgcagcaaca tcagtagcaa ccagtacacg ggcgctaccg ttagcaaaac
    61201 gtaccagagt ctgatcgcga tcgcgttgct ccagatcgcc gtgtaacgac aatgcacttt
    61261 gccctacttc attcagcgcg tcgcagacag cctggcaatc ttttttggta ttgcaaaaca
    61321 ccacgcaaga ggatggctga tgcaagctta ataaccgttg caacagaggg attttgcctt
    61381 tgctggatgt ctcataaaat tgttgttcaa tgggtggcaa agcatctgtt gagtcaattt
    61441 caatcgccaa aggatcgcgt tgtactcgtc cgctgattgc ggcgatggct tccggccagg
    61501 ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa acggatgaca tcatcaatgg
    61561 catcgctaaa tcccatatcc agcatgcggt cggcctcatc catcaccaaa gtattcagcg
    61621 catccagtga taccgtgcct ttttgcaagt gatccagcaa acgacccggc gttgccacga
    61681 taatatgcgg cgcatgttgc aacgaatcac gctgcatacc gaacggttga ccaccgcaca
    61741 acgtcaaaat tttggtattt ggcagaaaac gcgccagccg acgcaattca cctgccacct
    61801 gatccgccag ttcacgcgta ggacacagca ctaaagcctg ggtttgaaat agcgacgcat
    61861 caatttgctg taacaagccg aggccaaaag ccgccgtttt gccgctgccg gttttcgcct
    61921 gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc ggcggcctgc accggcgtca
    61981 tggttaaata acccaactca ttaaggttcg tgagttgggc gggaggcaaa acattcaggg
    62041 tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg caaagttagc aggcgcgtat
    62101 cctcgcagat ctacgctcac gatgcgacaa tttaatcggt tcttcatcgg gtggtgggtc
    62161 aggcatgggt tgcgggcgag ggatcggatc gggcactgga acaggatcgc taggaatcgg
    62221 ttcagggaca ggaatttgca aataaataag tgtcgtcata tttccctccg gtcattgggt
    62281 ggactcttaa agggtagacg ctgataaata acaggcaaaa aaaagccgac tcatcaaagt
    62341 cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa aaaggaagta agacaatatg
    62401 gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa tggagatatt gccctgaatg
    62461 ggtagagagt ttattgactt cgctcaaact ttgcggcgtt tttgtataca gacagccgga
    62521 aaaattgctt ttgttacaac catttactac ggtgcaacca taaagcaaca ccaccaataa
    62581 gaacaactaa cagaatacaa aaaattgaaa atccgaattg ccacccgccg ccagggatcc
    62641 caccaaggtt gacgccaaat aaccctgtca gaaaggtact gggtaaaaag accattgcca
    62701 tcaatgacat tgtataggta cgacgagcta aattttcctg catcacctga gcgatttcat
    62761 ccgccatcac accagtccgt gctatacagg cgtcgatttc gtcaaggccg cgcccaaggc
    62821 gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct catccacggc aaacgttcac
    62881 tggcaagacg agcatagaca tcacgttgcg gtgccatata gcgacgcatc acgattaatt
    62941 gtttgcgcag cagagccagg aatccacgcg gtggaatttg ctgatcaagg agattatctt
    63001 caaggtcgat aattttatcg tgcagctgct cgataaattc gctggaatga tcggtcaacg
    63061 catcgcacac atccaccagc caccctccgc aatcggtcgg acccgtgccc tcttccagat
    63121 cgctcaccac atcgtccagc gccagcactt tgcgttgtcg ggtcgaaaca attaaccgcc
    63181 cgtccatata tacacgcatg gcgaccagtt gatcggggcg ttcatcggtg ctgccgttta
    63241 tacagcgcaa tgtaataagc gtgccttcac cgagacggct gactcggggc cgtgtgctct
    63301 cgcccgccag cgcatcacgt acgttattgg gaagcagcgg tgttgtcgcc agccattggg
    63361 cgctatcatg gtgtacataa ttaaggtgga gccagcaggg atgcgcttca tcaatcacat
    63421 ctgtattttc cagcggttta acgccgcctc taccatccag catccaggca aatactgcat
    63481 ccgggacatt aacgtccgat cccttaatcg cttccacagt gcctccatca tcaacgcatt
    63541 attttgtagt ctagccttct ggccctgtta cgcaacatct catcacccca ttaccctgaa
    63601 atgattaata aaattctgtc taaattgaat acaaaaagca aaatgctttt ccgtatacaa
    63661 accgtgtgaa gtgttaaata gcgtctatca ttatcagaat tatctgatca tatgacgtgg
    63721 cttttttgcg atcggatagc aacaaaaatt gataaaaata acgggatctc aatgattacg
    63781 cacaacttca ataccctgga cttactcacc agtcctgtct ggattgtttc gccctttgag
    63841 gaacagttaa tttatgccaa tagcgcggcg cgactgttga tgcaagacct cacgtttagt
    63901 cagctacgaa ccggacccta ttccgtctcc tcacaaaaag aactgccgaa atacctctcc
    63961 gatctgcaaa accaacacga tattatcgaa atcctcactg ttcagcgtaa agaagaggaa
    64021 acagcattaa gctgtcggct tgttttgcga gagctgacag aaacagaacc ggtgattatt
    64081 ttcgaaggta tcgaagcgcc ggcaacgctg ggtttaaaag ccagtcgctc ggcaaattat
    64141 cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga ctaactctgc accaatgttg
    64201 ttgattgacc cctcacgaga tggccaaatc gtcgatgcta acctcgccgc gctcaatttc
    64261 tatggttata accatgaaac gatgtgccag aaacatacct gggaaattaa tatgctcggg
    64321 cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac ccggcggtca taaacctttg
    64381 aattttgttc ataaactggc ggatggatct actcgtcatg tgcagaccta tgccgggccg
    64441 attgaaattt atggcgacaa gctcatgtta tgtattgtgc atgatattac tgagcaaaaa
    64501 cggctggagg agcagctgga acatgctgct caccatgacg cgatgaccgg attactgaat
    64561 cggcgacagt tttatcacat taccgagcca ggccaaatgc agcacctcgc catcgcccag
    64621 gattacagct tgttgctcat cgacaccgat cgttttaaac acattaacga tctctatggg
    64681 cattctaaag gtgatgaggt gttatgcgcc ctcgcccgca ccctcgaaag ttgcgctcgc
    64741 aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg tcttattgct accaagaacc
    64801 ccactggata ccgcgctttc gctggctgaa actatccgtg taagcgtggc aaaagtgagt
    64861 atttcgggct taccacgttt taccgtcagc attggtgtgg cgcatcacga aggaaatgaa
    64921 agcatcgatg aactgtttaa acgcgttgat gatgctttgt atcgggcgaa aaatgatgga
    64981 cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc gagatcaacg gctgcgctta
    65041 gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt tacgtagtgc gacgtaacac
    65101 gccccgctgc cgccatgatg cggtagcgcg gtgcaatatg cctgaacatc atcaaattcg
    65161 gtcagccagc gcgccacata gctgcggaca atattggcat gcgatttatc atcccgtcct
    65221 ttgccatgaa taatcagcac gttacgcaaa ccatcagcca tggcttgttg aatgaaactg
    65281 aacatcattt tgcggcactc ttccaccggc tggcgcaaaa gattcaggct cgcctgttgc
    65341 ggatatttac cactgcgcag cttatccagc accccatgtt gcaacccttc ccgccgaaac
    65401 tccagcggct gacttagtgg gatgatgtcg agaaatccgg tggtgaggaa attatcaagc
    65461 tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg ttggatgcca gtggacatcg
    65521 gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa gaaacagcga tttgtcgtca
    65581 aggttcatgt tacatcctcc cgcaattaag agcgcgatat gataaccaga ccgggtcggt
    65641 ccaacaacgt cttacccaaa attccagtaa taagttccaa atattgccga tattttaagc
    65701 aaaatgc
//
LOCUS       DAZFWL020000010        65185 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFWL020000010 DAZFWL020000000
VERSION     DAZFWL020000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 65185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.010"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            337..1008
                     /locus_tag="ACHKNP_RS03610"
                     /old_locus_tag="pgaptmp_000722"
     CDS             337..1008
                     /locus_tag="ACHKNP_RS03610"
                     /old_locus_tag="pgaptmp_000722"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HGU4702948.1"
                     /translation="MDKVLDSALPSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1018..2214
                     /gene="yaiP"
                     /locus_tag="ACHKNP_RS03615"
                     /old_locus_tag="pgaptmp_000723"
     CDS             1018..2214
                     /gene="yaiP"
                     /locus_tag="ACHKNP_RS03615"
                     /old_locus_tag="pgaptmp_000723"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGU4702949.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2192..2773
                     /locus_tag="ACHKNP_RS03620"
                     /old_locus_tag="pgaptmp_000724"
     CDS             2192..2773
                     /locus_tag="ACHKNP_RS03620"
                     /old_locus_tag="pgaptmp_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU4702950.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2775..3548
                     /gene="yaiO"
                     /locus_tag="ACHKNP_RS03625"
                     /old_locus_tag="pgaptmp_000725"
     CDS             2775..3548
                     /gene="yaiO"
                     /locus_tag="ACHKNP_RS03625"
                     /old_locus_tag="pgaptmp_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HGU4702951.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQNFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3734..4009
                     /gene="frmR"
                     /locus_tag="ACHKNP_RS03630"
                     /old_locus_tag="pgaptmp_000726"
     CDS             3734..4009
                     /gene="frmR"
                     /locus_tag="ACHKNP_RS03630"
                     /old_locus_tag="pgaptmp_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HGU4702952.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSMEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4044..5153
                     /gene="frmA"
                     /locus_tag="ACHKNP_RS03635"
                     /old_locus_tag="pgaptmp_000727"
     CDS             4044..5153
                     /gene="frmA"
                     /locus_tag="ACHKNP_RS03635"
                     /old_locus_tag="pgaptmp_000727"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HGU4702953.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5246..6079
                     /gene="fghA"
                     /locus_tag="ACHKNP_RS03640"
                     /old_locus_tag="pgaptmp_000728"
     CDS             5246..6079
                     /gene="fghA"
                     /locus_tag="ACHKNP_RS03640"
                     /old_locus_tag="pgaptmp_000728"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU4702954.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6204..6743)
                     /gene="yaiL"
                     /locus_tag="ACHKNP_RS03645"
                     /old_locus_tag="pgaptmp_000729"
     CDS             complement(6204..6743)
                     /gene="yaiL"
                     /locus_tag="ACHKNP_RS03645"
                     /old_locus_tag="pgaptmp_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HGU4702955.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6845..8056)
                     /gene="mhpT"
                     /locus_tag="ACHKNP_RS03650"
                     /old_locus_tag="pgaptmp_000730"
     CDS             complement(6845..8056)
                     /gene="mhpT"
                     /locus_tag="ACHKNP_RS03650"
                     /old_locus_tag="pgaptmp_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HGU4702956.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGDKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8328..9341)
                     /gene="mhpE"
                     /locus_tag="ACHKNP_RS03655"
                     /old_locus_tag="pgaptmp_000731"
     CDS             complement(8328..9341)
                     /gene="mhpE"
                     /locus_tag="ACHKNP_RS03655"
                     /old_locus_tag="pgaptmp_000731"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HGU4702957.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9338..10288)
                     /gene="mhpF"
                     /locus_tag="ACHKNP_RS03660"
                     /old_locus_tag="pgaptmp_000732"
     CDS             complement(9338..10288)
                     /gene="mhpF"
                     /locus_tag="ACHKNP_RS03660"
                     /old_locus_tag="pgaptmp_000732"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HGU4702958.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ASARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10285..11094)
                     /gene="mhpD"
                     /locus_tag="ACHKNP_RS03665"
                     /old_locus_tag="pgaptmp_000733"
     CDS             complement(10285..11094)
                     /gene="mhpD"
                     /locus_tag="ACHKNP_RS03665"
                     /old_locus_tag="pgaptmp_000733"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HGU4702959.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11104..11970)
                     /gene="mhpC"
                     /locus_tag="ACHKNP_RS03670"
                     /old_locus_tag="pgaptmp_000734"
     CDS             complement(11104..11970)
                     /gene="mhpC"
                     /locus_tag="ACHKNP_RS03670"
                     /old_locus_tag="pgaptmp_000734"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HGU4702960.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHQENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11988..12932)
                     /gene="mhpB"
                     /locus_tag="ACHKNP_RS03675"
                     /old_locus_tag="pgaptmp_000735"
     CDS             complement(11988..12932)
                     /gene="mhpB"
                     /locus_tag="ACHKNP_RS03675"
                     /old_locus_tag="pgaptmp_000735"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HGU4702961.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12934..14598)
                     /gene="mhpA"
                     /locus_tag="ACHKNP_RS03680"
                     /old_locus_tag="pgaptmp_000736"
     CDS             complement(12934..14598)
                     /gene="mhpA"
                     /locus_tag="ACHKNP_RS03680"
                     /old_locus_tag="pgaptmp_000736"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HGU4702962.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDVL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14789..15622
                     /gene="mhpR"
                     /locus_tag="ACHKNP_RS03685"
                     /old_locus_tag="pgaptmp_000737"
     CDS             14789..15622
                     /gene="mhpR"
                     /locus_tag="ACHKNP_RS03685"
                     /old_locus_tag="pgaptmp_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HGU4702963.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15699..16781
                     /gene="lacI"
                     /locus_tag="ACHKNP_RS03690"
                     /old_locus_tag="pgaptmp_000738"
     CDS             15699..16781
                     /gene="lacI"
                     /locus_tag="ACHKNP_RS03690"
                     /old_locus_tag="pgaptmp_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HGU4702964.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLLINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16904..19978
                     /gene="lacZ"
                     /locus_tag="ACHKNP_RS03695"
                     /old_locus_tag="pgaptmp_000739"
     CDS             16904..19978
                     /gene="lacZ"
                     /locus_tag="ACHKNP_RS03695"
                     /old_locus_tag="pgaptmp_000739"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGU4702965.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTFATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWLAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            20030..21283
                     /gene="lacY"
                     /locus_tag="ACHKNP_RS03700"
                     /old_locus_tag="pgaptmp_000740"
     CDS             20030..21283
                     /gene="lacY"
                     /locus_tag="ACHKNP_RS03700"
                     /old_locus_tag="pgaptmp_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HGU4702966.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(21362..22543)
                     /gene="cynX"
                     /locus_tag="ACHKNP_RS03705"
                     /old_locus_tag="pgaptmp_000741"
     CDS             complement(21362..22543)
                     /gene="cynX"
                     /locus_tag="ACHKNP_RS03705"
                     /old_locus_tag="pgaptmp_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HGU4702967.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVSWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(22549..23019)
                     /gene="cynS"
                     /locus_tag="ACHKNP_RS03710"
                     /old_locus_tag="pgaptmp_000742"
     CDS             complement(22549..23019)
                     /gene="cynS"
                     /locus_tag="ACHKNP_RS03710"
                     /old_locus_tag="pgaptmp_000742"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HGU4702968.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23050..23709)
                     /gene="cynT"
                     /locus_tag="ACHKNP_RS03715"
                     /old_locus_tag="pgaptmp_000743"
     CDS             complement(23050..23709)
                     /gene="cynT"
                     /locus_tag="ACHKNP_RS03715"
                     /old_locus_tag="pgaptmp_000743"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HGU4702969.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            23819..24718
                     /gene="cynR"
                     /locus_tag="ACHKNP_RS03720"
                     /old_locus_tag="pgaptmp_000744"
     CDS             23819..24718
                     /gene="cynR"
                     /locus_tag="ACHKNP_RS03720"
                     /old_locus_tag="pgaptmp_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HGU4702970.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(24758..26041)
                     /gene="codA"
                     /locus_tag="ACHKNP_RS03725"
                     /old_locus_tag="pgaptmp_000745"
     CDS             complement(24758..26041)
                     /gene="codA"
                     /locus_tag="ACHKNP_RS03725"
                     /old_locus_tag="pgaptmp_000745"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HGU4702971.1"
                     /translation="MSNNALQTIINARLPGKEVLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26031..27290)
                     /gene="codB"
                     /locus_tag="ACHKNP_RS03730"
                     /old_locus_tag="pgaptmp_000746"
     CDS             complement(26031..27290)
                     /gene="codB"
                     /locus_tag="ACHKNP_RS03730"
                     /old_locus_tag="pgaptmp_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HGU4702972.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(27629..29515)
                     /gene="prpE"
                     /locus_tag="ACHKNP_RS03735"
                     /old_locus_tag="pgaptmp_000747"
     CDS             complement(27629..29515)
                     /gene="prpE"
                     /locus_tag="ACHKNP_RS03735"
                     /old_locus_tag="pgaptmp_000747"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HGU4702973.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(29555..31006)
                     /gene="prpD"
                     /locus_tag="ACHKNP_RS03740"
                     /old_locus_tag="pgaptmp_000748"
     CDS             complement(29555..31006)
                     /gene="prpD"
                     /locus_tag="ACHKNP_RS03740"
                     /old_locus_tag="pgaptmp_000748"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HGU4702974.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(31040..32209)
                     /gene="prpC"
                     /locus_tag="ACHKNP_RS03745"
                     /old_locus_tag="pgaptmp_000749"
     CDS             complement(31040..32209)
                     /gene="prpC"
                     /locus_tag="ACHKNP_RS03745"
                     /old_locus_tag="pgaptmp_000749"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HGU4702975.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASYPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIANRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(32254..33144)
                     /gene="prpB"
                     /locus_tag="ACHKNP_RS03750"
                     /old_locus_tag="pgaptmp_000750"
     CDS             complement(32254..33144)
                     /gene="prpB"
                     /locus_tag="ACHKNP_RS03750"
                     /old_locus_tag="pgaptmp_000750"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HGU4702976.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTIDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            33383..34969
                     /gene="prpR"
                     /locus_tag="ACHKNP_RS03755"
                     /old_locus_tag="pgaptmp_000751"
     CDS             33383..34969
                     /gene="prpR"
                     /locus_tag="ACHKNP_RS03755"
                     /old_locus_tag="pgaptmp_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HGU4702977.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIHKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRLGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(35067..35342)
                     /gene="yahO"
                     /locus_tag="ACHKNP_RS03760"
                     /old_locus_tag="pgaptmp_000752"
     CDS             complement(35067..35342)
                     /gene="yahO"
                     /locus_tag="ACHKNP_RS03760"
                     /old_locus_tag="pgaptmp_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HGU4702978.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35489..36160
                     /gene="yahN"
                     /locus_tag="ACHKNP_RS03765"
                     /old_locus_tag="pgaptmp_000753"
     CDS             35489..36160
                     /gene="yahN"
                     /locus_tag="ACHKNP_RS03765"
                     /old_locus_tag="pgaptmp_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HGU4702979.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36177..36422)
                     /gene="yahM"
                     /locus_tag="ACHKNP_RS03770"
                     /old_locus_tag="pgaptmp_000754"
     CDS             complement(36177..36422)
                     /gene="yahM"
                     /locus_tag="ACHKNP_RS03770"
                     /old_locus_tag="pgaptmp_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HGU4702980.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGQSSPMVGIRQIRLIT"
     gene            complement(36834..37649)
                     /gene="yahL"
                     /locus_tag="ACHKNP_RS03775"
                     /old_locus_tag="pgaptmp_000755"
     CDS             complement(36834..37649)
                     /gene="yahL"
                     /locus_tag="ACHKNP_RS03775"
                     /old_locus_tag="pgaptmp_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HGU4702981.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(37892..38941)
                     /gene="yahK"
                     /locus_tag="ACHKNP_RS03780"
                     /old_locus_tag="pgaptmp_000756"
     CDS             complement(37892..38941)
                     /gene="yahK"
                     /locus_tag="ACHKNP_RS03780"
                     /old_locus_tag="pgaptmp_000756"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HGU4702982.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(39028..39984)
                     /locus_tag="ACHKNP_RS03785"
                     /old_locus_tag="pgaptmp_000757"
     CDS             complement(39028..39984)
                     /locus_tag="ACHKNP_RS03785"
                     /old_locus_tag="pgaptmp_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4702983.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(39981..40952)
                     /locus_tag="ACHKNP_RS03790"
                     /old_locus_tag="pgaptmp_000758"
     CDS             complement(39981..40952)
                     /locus_tag="ACHKNP_RS03790"
                     /old_locus_tag="pgaptmp_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4702984.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(40945..42429)
                     /locus_tag="ACHKNP_RS03795"
                     /old_locus_tag="pgaptmp_000759"
     CDS             complement(40945..42429)
                     /locus_tag="ACHKNP_RS03795"
                     /old_locus_tag="pgaptmp_000759"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU4702985.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(42478..43461)
                     /locus_tag="ACHKNP_RS03800"
                     /old_locus_tag="pgaptmp_000760"
     CDS             complement(42478..43461)
                     /locus_tag="ACHKNP_RS03800"
                     /old_locus_tag="pgaptmp_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4702986.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGNAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDSASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(43715..44125)
                     /locus_tag="ACHKNP_RS03805"
                     /old_locus_tag="pgaptmp_000761"
     CDS             complement(43715..44125)
                     /locus_tag="ACHKNP_RS03805"
                     /old_locus_tag="pgaptmp_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGU4702987.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(44424..45806)
                     /gene="yahJ"
                     /locus_tag="ACHKNP_RS03810"
                     /old_locus_tag="pgaptmp_000762"
     CDS             complement(44424..45806)
                     /gene="yahJ"
                     /locus_tag="ACHKNP_RS03810"
                     /old_locus_tag="pgaptmp_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGU4702988.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(45816..46766)
                     /gene="yahI"
                     /locus_tag="ACHKNP_RS03815"
                     /old_locus_tag="pgaptmp_000763"
     CDS             complement(45816..46766)
                     /gene="yahI"
                     /locus_tag="ACHKNP_RS03815"
                     /old_locus_tag="pgaptmp_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HGU4702989.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(46909..48327)
                     /gene="yahG"
                     /locus_tag="ACHKNP_RS03820"
                     /old_locus_tag="pgaptmp_000764"
     CDS             complement(46909..48327)
                     /gene="yahG"
                     /locus_tag="ACHKNP_RS03820"
                     /old_locus_tag="pgaptmp_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU4702990.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(48327..49874)
                     /gene="fdrA"
                     /locus_tag="ACHKNP_RS03825"
                     /old_locus_tag="pgaptmp_000765"
     CDS             complement(48327..49874)
                     /gene="fdrA"
                     /locus_tag="ACHKNP_RS03825"
                     /old_locus_tag="pgaptmp_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU4702991.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(49864..50727)
                     /gene="yahE"
                     /locus_tag="ACHKNP_RS03830"
                     /old_locus_tag="pgaptmp_000766"
     CDS             complement(49864..50727)
                     /gene="yahE"
                     /locus_tag="ACHKNP_RS03830"
                     /old_locus_tag="pgaptmp_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU4702992.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(50767..51372)
                     /gene="yahD"
                     /locus_tag="ACHKNP_RS03835"
                     /old_locus_tag="pgaptmp_000767"
     CDS             complement(50767..51372)
                     /gene="yahD"
                     /locus_tag="ACHKNP_RS03835"
                     /old_locus_tag="pgaptmp_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HGU4702993.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELAREQGFEEIAQLLIAAGA"
     gene            51630..52127
                     /gene="yahC"
                     /locus_tag="ACHKNP_RS03840"
                     /old_locus_tag="pgaptmp_000768"
     CDS             51630..52127
                     /gene="yahC"
                     /locus_tag="ACHKNP_RS03840"
                     /old_locus_tag="pgaptmp_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGU4702994.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            52219..53151
                     /gene="yahB"
                     /locus_tag="ACHKNP_RS03845"
                     /old_locus_tag="pgaptmp_000769"
     CDS             52219..53151
                     /gene="yahB"
                     /locus_tag="ACHKNP_RS03845"
                     /old_locus_tag="pgaptmp_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4702995.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(53193..54281)
                     /gene="pdeL"
                     /locus_tag="ACHKNP_RS03850"
                     /old_locus_tag="pgaptmp_000770"
     CDS             complement(53193..54281)
                     /gene="pdeL"
                     /locus_tag="ACHKNP_RS03850"
                     /old_locus_tag="pgaptmp_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4702996.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(54423..58406)
                     /gene="ehaA"
                     /locus_tag="ACHKNP_RS03855"
                     /old_locus_tag="pgaptmp_000771"
     CDS             complement(54423..58406)
                     /gene="ehaA"
                     /locus_tag="ACHKNP_RS03855"
                     /old_locus_tag="pgaptmp_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HGU4702997.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNSHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSTEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            complement(58979..61012)
                     /gene="betT"
                     /locus_tag="ACHKNP_RS03860"
                     /old_locus_tag="pgaptmp_000772"
     CDS             complement(58979..61012)
                     /gene="betT"
                     /locus_tag="ACHKNP_RS03860"
                     /old_locus_tag="pgaptmp_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HGU4702998.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            61141..61728
                     /gene="betI"
                     /locus_tag="ACHKNP_RS03865"
                     /old_locus_tag="pgaptmp_000773"
     CDS             61141..61728
                     /gene="betI"
                     /locus_tag="ACHKNP_RS03865"
                     /old_locus_tag="pgaptmp_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HGU4702999.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            61742..63214
                     /gene="betB"
                     /locus_tag="ACHKNP_RS03870"
                     /old_locus_tag="pgaptmp_000774"
     CDS             61742..63214
                     /gene="betB"
                     /locus_tag="ACHKNP_RS03870"
                     /old_locus_tag="pgaptmp_000774"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HGU4703000.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGGGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFSPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            63228..64898
                     /gene="betA"
                     /locus_tag="ACHKNP_RS03875"
                     /old_locus_tag="pgaptmp_000775"
     CDS             63228..64898
                     /gene="betA"
                     /locus_tag="ACHKNP_RS03875"
                     /old_locus_tag="pgaptmp_000775"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HGU4703001.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRI
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            65111..>65185
                     /locus_tag="ACHKNP_RS03880"
                     /old_locus_tag="pgaptmp_000776"
     CDS             65111..>65185
                     /locus_tag="ACHKNP_RS03880"
                     /old_locus_tag="pgaptmp_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703002.1"
                     /translation="MREQIKQDIDLIEILFYLKKKVRVI"
BASE COUNT        15612 a        17685 c        16353 g        15535 t
ORIGIN      
        1 catcattctc aatcactcgc atattacatt cacataatgt atataaacaa tacttgttca
       61 cagaacatat tttttaaatg caattaattg acaaactaaa tcacatctaa atacaatgta
      121 taaaacaaaa taactataca tacaaaacat acaaacactt aacatatgaa ttgcataaaa
      181 acattaccca gtcgaacctc cgggtctgaa agcccttgct tttcagttat tagcaacatt
      241 taacatttat aaattaacat ctggctgcgc cagagattac gtctctgagt gcatcgttgt
      301 gcttgtagta taataaataa aatggagtgt cattaagtgg ataaggtttt agattcagcc
      361 ctcccttctt cggcaaataa aagaaagggt atccttgcta ttggcgcaca tcctgacgat
      421 atagaattag gctgtggcgc atcgcttgct cgtcttgcgc aaaaaggaat ttatatcgca
      481 gccgtggtga tgactaccgg caactctggt acagatggaa taatcgatcg ccatgaagaa
      541 tcgcgcaacg ccctaaagat attagggtgc caccaaacta ttcatcttaa ttttgctgac
      601 acccgcgctc atttacagct caatgatatg atttccgctc tggaagacat cattaaaaat
      661 caaattcctt ctgatgttga aatcatgcgg gtatatacca tgcatgatgc cgaccgccat
      721 caggatcatc tcgctgttta tcaagcttca atggttgcct gccgcactat tccacaaatt
      781 ctcggctacg aaaccccaag tacctggctt tcatttatgc ctcaggtttt tgaatccgtt
      841 aaagaagaat atttcacagt caaacttgct gcattaaaaa aacataaaag ccaggaacga
      901 cgtgattata tgcgtcatga tcgcctgcgt gcagttgcac aatttcgcgg gcaacaggtc
      961 aatagcgatc tgggtgaagg ctttgtaatt cataaaatga ttctttgagc aaattgcatg
     1021 aaaacctgga tatttatctg tatgtctata gcaatgttgc tatggttttt aagtacgcta
     1081 agacgtaaac ccagtcaaaa gaaaggctgt attgacgcca ttatacctgc gtataacgaa
     1141 ggcccgtgtc tggcgcagtc actggataat ctactgcgta acccttattt ttgccgggta
     1201 atttgcgtta acgacggctc cacggacaat accgaagcgg tcatggcgga agtcaaacgc
     1261 aaatggggcg accgctttgt tgccgtcacg caaaaaaata ccggtaaagg tggtgcgctg
     1321 atgaatggcc tcaattatgc cacctgcgac caggtttttt taagtgatgc cgacacctat
     1381 gttccgcccg atcaagacgg aatgggctat atgctggcag aaattgagcg cggtgctgat
     1441 gccgtaggcg gcattccctc tactgcgttg aaaggcgcgg ggctgttacc gcacatccgc
     1501 gcgaccgtaa agttgccgat gattgttatg aagcgcacgc tacagcagct cctgggcggc
     1561 gcaccgttta ttatcagcgg tgcctgcgga atgttccgta ctgatgtatt gcgtaagttc
     1621 ggtttctcgg atcgtactaa agtcgaagac cttgatctca cctggacatt agtggcaaac
     1681 ggctaccgta ttcggcaggc gaatcgctgc atcgtatacc cacaggaatg caacagcccg
     1741 cgtgaggagt ggcgtcgctg gcggcgttgg attgtgggat acgcggtctg tatgcgcctg
     1801 cataaaagac ttttatttag ccgcttcggt atcttcagta tatttcctat gctgttggtt
     1861 gtgctttatg gcgttgggat ttatctcact acctggttta atgaattcat caccaccggg
     1921 ccgcatggtg tggtgttggc aatgtttccg cttatctgga tcggcgtagt ttgtgttatt
     1981 ggtgctttta gcgcctggtt tcatcgttgc tggttgttgg tgcctttagc gccgctttcc
     2041 gttgtgtatg tattattagc ttatgccatc tggattattt atggacttat tgcctttttt
     2101 actggacgcg aacctcagcg cgacaaaccc acccgctatt ccgcactggt ggaagcgtca
     2161 accgcttatt cccaaccttc tgtcacagga actgaaaaac tatctgaagc ttaatgttaa
     2221 agagaaaaat attcagattg cagaccaggt aattattgat gaaactgcag gtgaagttgt
     2281 tatcggcgcg aatacccgta tttgtcatgg tgccgttatt cagggtccgg tagtgattgg
     2341 cgcaaactgc ctgataggta attatgcgtt tattcgtcct ggcacaataa tcagcaatgg
     2401 tgtaaaaatt ggttttgcca ccgaaattaa aaatgcggtt attgaagcgg aagcaacgat
     2461 tggtccgcaa tgttttattg ccgactcggt agttgcaaac caggcatatt tgggcgcaca
     2521 agtacgtacc agtaatcatc gtctggatga acagcctgtg tctgttcgaa ctccagaggg
     2581 aattatcgct accggatgcg ataaattagg ttgttatatc gggcagcgtt cacgccttgg
     2641 cgtacaagtt ataattttgc ctgggcgaat tatttccccg aacacgcaac ttggcccgcg
     2701 cgtgattgta gaacgtaatt tacctgccgg aacttattca ctccgacaag aacttatccg
     2761 tacaggagat taaaatgata aaacggacgt tattagcggc ggccattttt agcgcattgc
     2821 ccgcttatgc cgggttaact tccattaccg cgggctacga ttttaccgat tattctggcg
     2881 atcatggcaa ccgtaattta gcgtatgctg aactggtggc gaaagttgaa aacgcaacgc
     2941 tgctttttaa tctttcccag gggcgtcgtg attatgaaac tgaacatttc aatgccactc
     3001 gcggtcaggg tgcggtctgg tataaatgga ataactggct gacaacccga acgggtattg
     3061 cctttgcgga taatacgccg gtctttgccc gccagaattt tcgtcaggat attaacctgg
     3121 ccctgttgcc aaaaacgctt ttcacgaccg gttatcgcta cactaaatat tacgatgatg
     3181 tcgaagtcga tgcctggcaa ggcggcgtat cactctatac tggcccggtg atcaccagct
     3241 accgctatac ccattatgac tccagcgatg caggtggtag ttatagcaat atgatttccg
     3301 tgcgcctgaa tgacccgcgc ggcactggtt atacgcaact atggctaagc cgcggaacag
     3361 gcgcttacac ctatgactgg acgccagaaa cacgctacgg cagcatgaag agcgtcagtc
     3421 tgcaacgtat tcaaccgctg actgagcaac ttaatctcgg gctgacggca ggtaaagtgt
     3481 ggtacgacac cccaaccgat gattataacg gtctgcaact tgcagcccat ctgacctgga
     3541 aattctgatt ccttctgccg cccgctatcc ggggcggcct tccctgccga ttagcccccc
     3601 cctttcctct ttgttttccg accacattca ccggataaat tttattctcc agtgttatat
     3661 actatagggg ggtatgcatt gacatgtagt atacccccct atagtatatt gcgtgcagat
     3721 gatgaggtgc gaaatgccca gtactccgga agagaagaaa aaggtcctta ctcgagttcg
     3781 tcgtattcga gggcagattg atgctctgga acggtcgatg gagggtgatg ccgaatgccg
     3841 tgccatactc caacagatcg ctgccgttcg gggcgcggct aacgggctga tggcagaagt
     3901 gcttgaaagc catatccggg aaacgtttga ccgaaatgac tgctacagcc gcgaagtcag
     3961 ccaatccgtt gacgacacta ttgaactggt tcgagcctat cttaaatagc tgaatctatt
     4021 accatattga ggaagagcga gagatgaaat cacgtgctgc cgttgcattt gctcccggta
     4081 aaccgctgga aatcgttgaa attgacgttg caccaccgaa aaaaggtgaa gtgctgatta
     4141 aagtcaccca taccggcgtt tgccataccg acgcatttac cctctccggg gatgacccgg
     4201 aaggtgtatt cccggtggtt ctcggtcacg aaggggccgg cgttgtggtt gaagtcggtg
     4261 aaggcgtaac cagcgtcaaa cctggcgacc atgtgatccc gctttacacc gcggagtgcg
     4321 gcgagtgtga gttctgtcgt tctggcaaaa ctaacctctg tgttgcggtt cgcgaaaccc
     4381 agggtaaagg cttgatgcca gacggcacca cccgtttttc ttacaacggg cagccgcttt
     4441 atcactacat ggggtgctct acattcagtg aatacaccgt ggtcgcggaa gtgtctctgg
     4501 ccaaaattaa tccagaagca aaccatgaac acgtctgtct gctgggctgt ggcgtgacca
     4561 ccggtattgg cgcggtgcac aacacagcta aagtccagcc aggtgattct gttgccgtgt
     4621 ttggtcttgg cgcgattggt ctggcagtgg ttcagggcgc gcgtcaggcg aaagcgggac
     4681 ggattatcgc tatcgatacc aacccgaaga aattcgatct ggcgcgtcgc ttcggtgcta
     4741 ccgactgcat taacccgaat gactacgaca aaccgattaa agatgtcctg ctggatatca
     4801 acaaatgggg tatcgaccat acctttgaat gcatcggtaa cgtcaacgtg atgcgtgcgg
     4861 cgctggaaag tgcgcaccgc ggctggggtc agtcggtgat catcggggtg gcaggttctg
     4921 gtcaggaaat ctccacccgt ccattccagt tggtcactgg tcgcgtatgg aaaggttccg
     4981 cgtttggcgg cgtgaaaggt cgttcccagt taccgggtat ggttgaagat gcgatgaaag
     5041 gtgatatcga tctggaaccg tttgtcacgc ataccatgag ccttgatgaa attaatgacg
     5101 ccttcgacct gatgcatgaa ggcaaatcca ttcgaaccgt aattcgttac tgatttcccg
     5161 caggtatacc ccgtccactt cagacggggt tcttaatact ctccctgggc agccgtccgg
     5221 gggattaacc gtgagataat gactgatgga actcattgaa aaacatgcca gctttggcgg
     5281 ctggcaaaat gtgtatcggc attattccca atcactgaaa tgtgaaatga atgtcggcgt
     5341 ctatctccca ccaaaagccg cgaatgaaaa attgccggtg ctgtactggc tttcgggcct
     5401 gacctgcaac gagcagaatt tcattactaa atcggggatg cagcgttacg cggctgaaca
     5461 caacattatt gttgttgcgc cggacaccag tccgcgaggc agtcatgtcg cagatgccga
     5521 ccgttacgat ctcgggcaag gtgccgggtt ttacctgaac gcaacgcaag cgccgtggaa
     5581 tgaacattac aaaatgtatg actatatccg caacgagctg ccggatttag tgatgcaaca
     5641 ttttccggca acgaccagaa agtctatctc tggtcattct atgggcgggc tgggcgcgct
     5701 ggtgctggcg ttacgtaacc cagatgaata tgtcagcgtc tcggcgtttt cgcccattgt
     5761 ctccccatcg caagtaccgt ggggacagca agcctttgcc gcatatcttg gtgaaaataa
     5821 agatgcctgg ttgaattacg atccggtgag tcttatttca caaggtcaac gcgttgcgga
     5881 aatcatggtt gatcaggggt tgagtgatga tttttacgcg gaacagctgc ggactccaaa
     5941 tcttgaaaag atctgccagg agatgaatat caagacgtta atccgttatc acgagggtta
     6001 cgaccacagc tattattttg tctccagttt tattggcgag catattgcct accacgccaa
     6061 taaactgaat atgcgttgat aatagtgcac gactgccgga tgcggcgtga acgccttatc
     6121 cgcctacatt tcgcccgcag gccgtaggca tgataagacg cgcatagcgt cgcatcaggc
     6181 atctgcgcac gacttatcta tgtttaccac atcaaatcat caggcacttt gaagtcggca
     6241 tatgggtcgt cttcatcctg ctcttcggcg ctgagcgcgc tgtgcaacac tatactgctg
     6301 gcatcacgct gagctatttt atcggcaacg ctggcgggaa taatggcgta ttcaccttcg
     6361 ctattgttat cgaccaacaa gcgagcaatc gccagacgac cattaatcaa ctgcgcctgt
     6421 gtgagcttat cgacaaaaat cttcttaatc agattgccgt cagtgaagtt aaaaccaata
     6481 tcgccattgg caatggtgat tcggttcatt tcaatgagct gcttcacctg agctttatat
     6541 tctttcgcca acgcagcttg tttttgttgc tcgctaagct gtttatcacg ctcaagctgt
     6601 gcctttttat tttcttctac cgccgcccga gcttcacgcg cctgaacgcg cgattttttc
     6661 gccgttctct ccaccttcgc cgcttttttg ctggtgacta atcctgcttt gagcaactgc
     6721 tcttgtaagg taagttttgc catctttgtt tccaaacccg ctgaataatt tttagcgagt
     6781 atacctgtaa acgccgtggg tgtattgggg gcaactccct ttcgcccctg cttaataagg
     6841 cgcgtcaggc atcggcacac ggctgcattc ttgatctccg actcatcaaa ataaacaccg
     6901 ccaacccagc aacaagaata cccggtgcag aagcggccat tacgccgacc gtgccagtgc
     6961 ctaatgccag cattttcccg gccagtaacg gaccgctcat agcccccaga cgccctaccg
     7021 ccacggctgt tcccacacct gttgcgcgga tctgcgaact gtaaaacaac ggtgccaggg
     7081 catacaaaac gctttgccca cctgtcgcaa acaaccccgc gacaaatccc gccagcaaca
     7141 taccgttaaa tgacgacacc gttccaagtg ccagcagcga agctaacatg ccgctataaa
     7201 tcagtagcga catggtcact ggacgcagct tatccatcaa tgcgcccaac attaacgtcc
     7261 cgcttgccgc acccatttgc agggcaaaca tcacccctgc cgcctgcgat ggctggaatc
     7321 cttgctccac caaaagcagc ggtagccagt tgatcaacat gtagaccacc agcagagtga
     7381 agaaataaca caaccacagc agcagcgtcg cggttgccgt ttctggcgca aataaggcac
     7441 gcagtggtgg cgcagcctgt ttatcgccag cgaaaaccgc cgactccggc agccagcgca
     7501 ttaatagcgg caccagaatc aacggcacca caccacctac ccaaaacacc gtttgccatg
     7561 ctaagtttgc ccccgcgaaa cccagtgtcg ccgccagcgc cgcgccaatg ggaacaccgc
     7621 aatacatcag gctcactgct gtcccacgaa aacgtggacc cgcggcttca gacgtcaggg
     7681 cgataagatt cggcaacgcc gcccccagcc cgacaccggt catcagccgc gcaaagacca
     7741 gtgaggggaa atcccaggca atcgccgttg ccagcgagaa caaaccaaac agcgcaactg
     7801 agccaatcaa aatgcgcttg cgaccataac ggtccgccag cattccgcca accaacgcgc
     7861 cgggtagcaa accgagtatt ccggcgctga atatccagcc catttgcatt ttatcgagtg
     7921 cgaaagcctg ggcgatgcca cccgccgcaa tgccagccgc ctgaagatcc agcccttcca
     7981 tcagagcgac caaaaaacaa agcccgatag tcagcatcag gcgggatgaa gatgatgaag
     8041 gggtacgagt cgacataaga tgaacctcgt tgcgagtgca ggtgaatcgc gactgataca
     8101 ttgccagccg cgtaatcaga actatttaat gactgcctac gggcgaaatg taggccggac
     8161 aagacgttta tcgcatccgg cagtcgtgtg cagatgcctg atgcgacgct aacgcgtctt
     8221 atcaggccaa attctcacgc cacccgtagg ccggataagg tgtttacgcc gcatccggca
     8281 gtcgtgctgg gatgggcata caaaatgcac gttctcggca gtcatcatta tttgttgttg
     8341 cgcagatcca gcgccacgtc aacgatcata tcctcctggc cgccaaccat ccggcgtttg
     8401 cccagctcaa cgagaatatc caccgcactt aagccataac gcgccgccgc cgtttcacag
     8461 tgacgcagga agctcgagta aacaccagcg tatcccagcg ccagcgtttc gcgatcgact
     8521 cgtaccggtc gatcctgcaa cggacgcacc aggtcgtcag cggcatccat taacgcatag
     8581 agatcggtcc catgctgcca gcccagttta tccgcggcgg caataaatac ttccagcggc
     8641 gcgttacctg cgcccgcgcc cattcccgcg aggctggcgt cgattcggtc gcagccctct
     8701 tccaccgccg cgatagagtt cgccacgcca agactcaggt tatggtgagc gtgcatgcca
     8761 gtttgcgttt ctggtttcag ctctgctttc agggcgcgga aacggtcacg gatatcgctc
     8821 atgttcatcg caccgccaga atccaccaca taaatacagg tcgcaccgta gccttccatc
     8881 agctttgcct gctttgcgag attctccggc gtggtcatat ggctcatcat cagaaaacca
     8941 acggtgtcca ttccgagctc gcgggcatac tgaatatgct gggcggaaac atcagcttcg
     9001 gtacagtgcg ttgccacacg aaccacccgc gcgccagcct gccaggcatt tttcagatcg
     9061 tgaatagtgc cgattcctgg cagcaacaac gtcgcgattt tggcgtgctt caccacatcc
     9121 gccgctgctt caatccattc aaggtcgcta tgtgcgccga aaccatagtt aaagctggaa
     9181 ccctgcaaac cgtcgccatg ggccacttca atcgaatcca cgcgggcatc gtccagtgct
     9241 ttggcaatct ggcgaacgtt ttccagcgaa tactgatgac gaatggcgtg cataccgtca
     9301 cgcaacgtga cgtccgagat ataaagtttt ttaccgttca tgccgcttct cctgccttgc
     9361 gcgccagtga ctgggccatt ttttccgctg tcgccagcgc actggaagtc ataatgtcga
     9421 ggttgcccgc ataggcaggc agataatgcg ctgcgccttc gacttccagc cagaccgctg
     9481 ttttcagtcc ggagaattgc cccacgcccg gtaaattgac cggtttatcc tgcgggataa
     9541 cttcaaactg cacgcgctgt ttcaggcgat aacccggtac gtaagcctgc accgcctcag
     9601 ccatttcatt gattgaggct tcgatatcat cttgtgaagc ttcgtcgctc aatacataca
     9661 ccgtgtcacg catcatcaac ggtggctctg ctgggttaag cacaataatc gccttccctt
     9721 ttgccgcgcc gcccaccact tcaatggctc gggaagtggt ttccgtaaat tcatcgatat
     9781 tggcacgcgt gccaggtccg gcagatttac tggcgataga agcgataatt tcggcgtaat
     9841 gaacacgcgc cacgcgtgaa actgccgcca ccattggaat ggtggcctgg ccgccgcagg
     9901 tgaccatgtt gacgttcagt tgatcgacgt tcgcctcgag gttaaccacc ggcacgcagt
     9961 aagggccgat ggcagcaggc gtcaggtcaa ttaagcgaat atccggtttc gcttcgcgta
    10021 aagcggcatc gtttttcaca tgcgcgcccg cgctggtcgc atcaaataca atgtcgatat
    10081 cagcaaattc aggcatgttc atcagcccga tcaccccttc atgggtggtg gcgacgccca
    10141 tacgtctggc actcgccaga ccgtcggact gaggatcaat gccgaccatc accgccatct
    10201 ccagatgctg accgtgacgc aaaattttaa tcatcagatc ggtgccaatg ttgccagaac
    10261 cgataatggc gactttacgc ttactcatga cagacttcct tttggggctg cgcttgaaaa
    10321 tgtcgcagca actgaaccta tgccttcaat atgggcttca aaacgatcgc ccgcattcac
    10381 cgccaccatc gggcctaatg ccccggtaag aatgatatct ccggcgcgca gcggttcacc
    10441 cagactggcc attttgcgtg ccagccagac ggcggcatta agcggatgtc ccaggcattc
    10501 gctgccgcgc ccgctggaaa cctcttcgtt attacgcgtc atcttcatgg cgcagttttt
    10561 caggtctaac cccgccggac gttgcgccgg accgccgatg acatacaccc cgcaggaagc
    10621 gttatctgcc acggtatcga caaactgaat cgaccagtcg cgaatgcggc tccccaccac
    10681 ttccagcgcc ggaagtaccc attcaatggc gttatacagt tcgtcgaagg tgatatcagt
    10741 tgcgggtaaa tcgcggttca acaccagtgc gatctccgct tcaatgcggg gttgcagaac
    10801 acgggaaaaa ggaatgattt cgttatcgcc ataacacatg tcggcaaata acgtcccaaa
    10861 atccggttga tcaacgccca gttgttgttg cactttcgga tgcgtcaggc ccactttacg
    10921 ccctaccacg cgacgcccct gcacaacgtc atattgcaca tttatgtgct gaatggcgta
    10981 ggccgcttca gcgttatcga taccaatcag atcgcgcagc ggcgcaatcg cttcgccctg
    11041 ctctgcggcg cggcgtaaat ccgccgccag ttgctcaaga gtatgcttcg tcatgaccat
    11101 tccttaaggg cgtgcgagga aattcagcac caattgattg aaagcgtcgg catgttccca
    11161 ctgcgcccag tgaccacagt cgcggaagat atgcagttca gaaccggcaa tgccggacag
    11221 cagacgcaga cccgcatcca tcggcacaaa gcggtcgttg cgcccccaga caatcagggt
    11281 ttgcgctttg atttccgcca gacgtgggcc aaaatccggg aactgtttcg gattagcttc
    11341 caggctctta acgaagtttt cctggtgatc gcggcgcgac agcatattat tcaggcgcgc
    11401 ttcaaacagg gcgtcggtca aatcgctggt atcgaaaacg aagatatcca tcatcagctt
    11461 caggttttcg atagtcggct gacgataaag ctgattcagt cgcttaatac cttcggttgg
    11521 catcggcgta aacaaactca tgccgcccgt accgccgccc atcagcacca gtttgccgac
    11581 gcgctccggc cagttaaggg tgaacgccac agaactatgg ccgcccatcg agttgcccag
    11641 caggtggatt ttggcgatat ccagttgatc caccacgctt ttcaggattc gtgcattaag
    11701 atccgatcgt gaaccactat taacgatcga atcgcttttg ccccaacccg gacaatccag
    11761 caggattacc cgatagcccg cctctaccag cggatcgata ttgcggctga agttcgccca
    11821 gccagtagca cccgggccgg aaccatgcag caggacaacg gtttcgtcgc cttgtccgca
    11881 gtcattaaaa tggatgcgca gcgttttacc cgcttcttct acattcagaa aacggctggt
    11941 ggcggcttcg gtttgtggct gataactcat catcttctcc tgcatattca gttctctgtt
    12001 ctggcgctta acgagccaaa tccggcaatc cactccggga ttgggcggta ataacgccct
    12061 tcgctacgcc agttgccaaa tgtagaaata gcggcaaaag cggcgaccca ggttttgatt
    12121 tcatgtgtcg actttccggc aatggcggaa agctcttcgt tactgacggc atccagttcc
    12181 tgtatgcgtc cctgctccag caaagtcatg aactggttat cccaaatcgg gttgagcgga
    12241 tgcagagttc tctgatcctc aacaaacttc tcagcggcgc taatcacccg ttgctgacgc
    12301 aattcgcgct cactggcggg taaatctttc ccgctcccca acagacggtc gcgcatatgg
    12361 gcatcggctt tcgccagttc gggcaccggc ggctgatggg aaagcccacc ggaacccagg
    12421 aacagcacgc gtttattgag agtgctggtg aaacgtccaa tggcttcacc caacatgcgg
    12481 gtacgctgga aaccgggcag cggcgtggcg acaccgttga tgaacacagg cagaactggc
    12541 accttatcca gcccaccgag caggaactcc agcggctggg cgaacccgtg gtccacctgc
    12601 atacagtaag aaacggcaag atcgatcccg ctcttcatga cagcatgcgc acaggcctcc
    12661 gccagctcca caggcacggg cagctctcct gccgcactgc cgaaatcacc aattgccgtc
    12721 gctccaacgc ctaaacagaa cggtggcatc acgtcataga aaaagccgtt gtagtgatct
    12781 ggcgcaaaca gcaccaccag ttcaggggag aaagccgcaa tacgctcgcg ggcgctggca
    12841 atcacgccat tgacctcatc gagtacctct tgcgccgggt cgacataccc caccaacggc
    12901 gagtgggaaa gacagtgaag ataagcgtgc atatcaggct accttttcga cagaaacgtc
    12961 ggcatcaggg cgggtcagcg tcatcaccga cgccagttta ttcagcgtat tgcccagggt
    13021 ttgcggaatg gcggtggcgg caacaaagcg atccgggcgc atcaccacca gcgaagcatt
    13081 gtgttgcgcg aaccagctac gcagacgacc ttgcgtatcg cccacgcgta gcacgccgtc
    13141 gtggttatcc tgtgcggtat gaatttgcac ttccggcacc acctgaatga agcgtgtgcc
    13201 caacgcgcgc cactgctgga tttgctcgtc gctcatcccc cacagtggat tgcatcccca
    13261 gccaattacc gcgaagttcg cgccaatcgc gttatcgagc agcgtcacgt cgccgttttc
    13321 cagcgtgact ttcggctgaa taaacatctt gccgaccgga gagtgcttcg cttcgccctc
    13381 acgtaccagc gcaccgccgt aatattgcgg catcggcttg aagcgcattt cgaggaagta
    13441 gcgttttact ggcggcagat aattcagtag ccaggaaacg ccgtcacgta acgtaccctg
    13501 ccagcgtttc ggcggagcca gcacgttgcc cgccgtcacg gacaggtcaa tcatcgcttt
    13561 ggcgtgatcg cgacgttctt gttgataggt atcgagcagc acatcgcggg ctttcccctg
    13621 gataaccaac gccagtttcc aggcgaggtt aaaggcgtcg cgcataccac tgttatagcc
    13681 ctgcccctgc cacaccggca tgatgtgcgc ggcatcgccc gccagcagta cgcgatcaat
    13741 acggaaacgt tgcgccagtc gcgcgttatg ggtgtagaca cgctggcgaa tcaattcaac
    13801 attgtccgga ttaggcagca ctttgcttaa cagcttgcgc atattttgcg gctcacgcag
    13861 ctgctcttcg gtttctcccg gcatcaccat aaattcaaag cgacgtaccg catgaggcag
    13921 cgcggcagaa acatacgggc gcaccggatc gcaacacaaa tagatatgcg gcgtacttaa
    13981 cggatcgttg gcgatatcta ccacaatcca ctgatttggc gcagttttac cttcaaacgg
    14041 cacattcaga gtgcgacgga caaaacttgc tccaccgtca caggctacca gccactgggc
    14101 tttgactatt tcccgctgcc cttctgccgt tttcaggtgc aaggtcactt cgtcattttg
    14161 ctggctgaag gcctccagct cgcgggaaaa caagcagcgc acattaggaa aacgcgacac
    14221 cccttccagc atcaccgcat cgacctgcgg ctgaataaag gcgttacggc gcggccagcc
    14281 aaattcatcg gtcattggct gaatatcagc aaaacagcgg cctttcgggg tgagaaaacg
    14341 catcgcgtgc cacggcgtag tgtgcggcag aacattctcg accaggccaa ccgactgcat
    14401 ggtgcgcagc gcctcgtcat caataccaat cgcacgcggg tagtcgatca acttatcgag
    14461 tttctccacc accagcacgt caatgcccat ctgaccgaga tagttcgcca tcatcagccc
    14521 gaccggaccg gcacctgcga tcgccacctg aacgctatgg ttaacagcag gctggatgtc
    14581 agggtgttgt attgccattt cagtacctca cgactcggac aaaatgccgt tgcgcgcaca
    14641 gtacggcgca acttattttg ttaaaaacat gtaaatgatt ttttattgtg cgctcagtat
    14701 aggaagggtg ttttcggcta caatcaaaac atgcccgaat gtgcaccagg tgcacaacgt
    14761 tgttttaact atagaaatgt caattaatat gcagaacaat gagcagacgg aatacaaaac
    14821 cgtgcgcggc ttaacccgcg gtctaatgtt attaaatatg ttaaataaac ttgatggcgg
    14881 tgccagcgtc gggctgctgg cggaactcag cggcctgcat cgcaccactg tgcggcgact
    14941 gctggagacg ctgcaggaag agggatatgt ccgccgtagc ccctccgatg atagttttcg
    15001 actgaccatc aaagtgcggc aattaagcga aggatttcgt gacgaacagt ggatttctgc
    15061 actggcggcc ccactgctgg gcgatctgtt gcgcgaagtg gtatggccga cagatgtgtc
    15121 cacgctggat gttgatgcaa tggtggtacg cgaaaccact caccgtttca gccgcttatc
    15181 ctttcaccgg gcaatggtcg ggcgacgttt gccgcttctg aaaaccgcct cgggcctgac
    15241 ctggctggcc ttttgcccgg aacaagaccg caaggaatta atcgaaatgt tagcctcccg
    15301 ccccggtgat gactatcaac tggcacggga accgttaaag ctggaagcca ttctggcgcg
    15361 cgcgcgcaaa gagggttacg gacagaacta ccgcggctgg gatcaggagg agaagatcgc
    15421 ctctatcgcc gtaccgctgc gcagtgaaca acgggtgatt ggctgtctga atctggtgta
    15481 tatggcgagc gcaatgacca ttgaacaggc agcggaaaag catcttccgg cgctacaacg
    15541 ggtagcaaaa cagatcgaag aaggggttga atcgcaggct attctggtgg ccggacggcg
    15601 aagcggcgtg catttacgtt gacaccatcg aatggcgcaa aacctttcgc ggtatggcat
    15661 gatagcgttc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgctatacg
    15721 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
    15781 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
    15841 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
    15901 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgctg
    15961 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
    16021 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgctcatt aactatccgc
    16081 tggatgacca ggatgccact gctgtggaag cagcctgcgc taatgttccg gcgttatttc
    16141 ttgatgtctc tgaccagact cccatcaaca gtattatttt ctcccatgaa gacggtacgc
    16201 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
    16261 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
    16321 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
    16381 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
    16441 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
    16501 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
    16561 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
    16621 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
    16681 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    16741 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    16801 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    16861 tgtgaaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgga
    16921 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cacaacttaa
    16981 tcgccttgcg gcacatcccc ctttcgccag ctggcgtaat agcgaagaag cccgcaccga
    17041 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc
    17101 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt
    17161 cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta
    17221 tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct
    17281 cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg
    17341 cgttaactcg gcgtttcatc tgtggtgcaa cggtcgctgg gtcggttacg gtcaggacag
    17401 tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc
    17461 ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa gatcaggata tgtggcggat
    17521 gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt
    17581 ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca
    17641 gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac
    17701 gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtagtta
    17761 tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat
    17821 cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc
    17881 agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa
    17941 cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca
    18001 ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa
    18061 cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta
    18121 cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg
    18181 tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca
    18241 gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgagt caggccacgg
    18301 cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca
    18361 gtatgaaggc ggcggagccg acaccttcgc aaccgatatt atttgcccga tgtacgcgcg
    18421 cgtggatgaa gaccaaccct tcccggcggt gccgaaatgg tccatcaaaa aatggctttc
    18481 gctgcctgga gaactgcgcc cactgatcct ttgcgaatat gcccacgcaa tgggtaacag
    18541 tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg
    18601 cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg
    18661 gttggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct gcatgaacgg
    18721 tctggtcttt gccgaccgca cgccgcatcc ggcgctgacg gaagcaaaat accagcagca
    18781 gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg
    18841 tcatagcgat aacgagctcc tgcactggat ggtggcgctg gatggcaagc cgctggcaag
    18901 cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctggact
    18961 accgcagccg gagagcgccg gacaactctg gcttacggta cacgtagtgc aaccgaacgc
    19021 gaccgcatgg tcagaagccg gacacatcag cgcctggcag cagtggcgtc tggcggaaaa
    19081 cctcagcgtg acactccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat
    19141 ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgtcagt caggccttct
    19201 ttcacagatg tggattggcg atgaaaaaca actgctgacg ccgctgcgcg atcagttcac
    19261 ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc
    19321 ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg
    19381 ctcggcagat acacttgccg acgcggtgct gattacgacc gctcacgcgt ggcagcatca
    19441 gggaaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc
    19501 gattaccgtt gatgttgaag tagcgagcga tacaccgcat ccggcacgga ttggcctgac
    19561 ctgccagctg gcgcaggtcg cagagcgggt aaactggctc ggattagggc cgcaagaaaa
    19621 ctatcccgac cgccttactg cggcctgttt tgaccgctgg gatctgccat tgtcagacat
    19681 gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa
    19741 ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca
    19801 gcaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa
    19861 tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtgtcggc
    19921 ggaattccag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataata
    19981 ataaccgggc aggccatgtc tgcccgtatt tcgcgtaagg aaatccatta tgtactattt
    20041 aaaaaacaca aacttttgga tgttcggttt attctttttc ttttactttt ttatcatggg
    20101 agcctacttc ccgtttttcc cgatttggct acatgacatc aaccatatca gcaaaagtga
    20161 tacgggtatt atttttgccg ctatttctct gttctcgcta ttattccaac cgctgtttgg
    20221 tctgctttct gacaaactcg ggctgcgcaa atacctgctg tggattatta ccggcatgtt
    20281 agtgatgttt gcgccgttct ttatttttat cttcgggcca ctgttacaat acaacatttt
    20341 agtaggatcg attgttggtg gtatttatct aggcttttgt tttaacgccg gtgcgccagc
    20401 agtagaggca tttattgaga aagtcagccg tcgcagtaat ttcgaatttg gtcgcgcgcg
    20461 gatgtttggc tgtgttggct gggcgttgtg tgcctcgatt gtcggcatca tgttcaccat
    20521 caataatcag tttgttttct ggctgggttc tggctgtgca ctcatcctcg ccgttttact
    20581 ctttttcgcc aaaacggatg cgccctcttc cgccacggtt gccaatgcgg taggtgccaa
    20641 ccattcggca tttagcctta agctggcact ggaactgttc agacagccaa aactgtggtt
    20701 tttgtcactg tatgttattg gcgtttcctg cacctacgat gtttttgacc aacagtttgc
    20761 taatttcttt acttcgttct ttgctaccgg tgaacagggt acgcgggtat ttggctacgt
    20821 aacgacaatg ggcgaattac ttaacgcctc gattatgttc tttgcgccac tgatcattaa
    20881 tcgcatcggt gggaaaaacg ccctgctgct ggctggcact attatgtctg tacgtattat
    20941 tggctcatcg ttcgccacct cagcgctgga agtggttatt ctgaaaacgc tgcatatgtt
    21001 tgaagtaccg ttcctgctgg tgggctgctt taaatatatt accagccagt ttgaagtgcg
    21061 tttttcagcg acgatttatc tggtctgttt ctgcttcttt aagcaactgg cgatgatttt
    21121 tatgtctgta ctggcgggca atatgtatga aagcatcggt ttccagggcg cttatctggt
    21181 gctgggtctg gtggcgctgg gcttcacctt aatttccgtg ttcacgctta gcggccccgg
    21241 cccgctttcc ctgctgcgtc gtcaggtgaa tgaagtcgct taagcaatta atgtcggatg
    21301 cgccactggg cacatccgac aattatttat agctccatca tctggcgaaa caagcgtcgc
    21361 atcatacctc tttgacccac agctgcggaa aacgtgctgg tgcaaaacgc agggttatga
    21421 tcatcagccc aacgacgcac agcgcatgaa acgcccagtc catcaggtaa ttgccgctga
    21481 tactacgcag cacgccagaa aaccacgggg caagcccggc gatgataaaa ccgatcccct
    21541 gcataaacgc caccagcttg ccagcaatag ccggttgcac agagtgatcg agcgccagca
    21601 gcaaacagag cggaaacgcg ccgcccagac ctaacccaca caccatcgcc cacgataccg
    21661 gcaattgcag cggcaaccag ataaagccgc agaaccccac cagttgtaac accagcgcca
    21721 gcattaacag tttgcgccga tcctgatggc gagccatagc aggcatcagc aaagctcctg
    21781 cggcttgccc aagcgtcatc aatgccagta aggaaccgct gtactgcgcg ctggcaccaa
    21841 tctcaatata gaaagcgggt aaccaggcaa tcaggctggc gtaaccgccg ttaatcagac
    21901 cgaagtaaac acccagcgtc cacgcgcggg gagtgaatac cacgcgaacc ggagtggttg
    21961 ttgtcttgtg ggaagaggcg acctcgcggg cgctttgcca ccaccaggca aagagcgcaa
    22021 caacggcagg cagcgcccac caggcgagtg tttgatacca ggtttcgcta tgttgaacta
    22081 accagggagt tatggcggca ccaagtccac cgccgcccat cagagccgca gaccacagcc
    22141 ccatcaccag tggcgtgcgt tgctgaaacc gccgtttaat cactgaaggc atcaccgcct
    22201 gaatgatacc gatccccacc ccaccaagca gtgcgctgct aagcagcagc acactttggg
    22261 ggtaaagctc acgcatcaat gcaccgacgg caatcagcaa cagactgatg gcgacactgc
    22321 gacgttcgct gacatgctga tgaagccagc ttccggccag cgccagcccg cccatggtga
    22381 ccaccggaag agcggtcaac agggcagcca cgctaaagct cattccgctc gcctggcgca
    22441 attgcggtag cagtggcccg acggaggtga gcagtggtcg catattaaga ccaatcagca
    22501 ccagaaccag cagcatcgtg aacgtctttt gatgattgcg catggctgtc agaacggctt
    22561 ggtcggcaga tatttaccat ctaaggtaat gaccgcacgt tcgccacctt ccgggtccgc
    22621 cactttctta acgtcgagtt tgaagttaat cgcgctgata atgccatcgc caaacttctc
    22681 atgaaccaac gctttcaggg ttgtgccgta cacctgcaac atttcataga aacgatacat
    22741 cgttgggtcg gttggaatac ggtcatcaat gcagccacgc agtggaatca tctgcaacag
    22801 tagaatggcg tcttcgtcga gatccagctt cgccccgacc aggcgggcgg cgtcggcagg
    22861 aagcgcttgc tgccccagca aagccgcggt tacgaaggct tctgccagac cggtaccgtc
    22921 ggcaatctcg gcaaatgaga gatctttttt agctttgctg agcaaaatgg catcggcaag
    22981 atcaagacga atattgcggt taatttgtga ctgaatcatg gtggatctcc tgatggttta
    23041 aaaataaggt tacgctgcgg tcggttggcg tagcggtatg gcacaaacgc gaggattagc
    23101 ggccagtggc acaaactggc gggttgcgcc gtcaaaagct gcgatgctgc cgctttcaat
    23161 gtcgtagacc cagccgtgca gggcgatccg cccctcttcg agcgccaggc gcaccgatgg
    23221 atgagtttgc aaattagcca actgagcaat gacgttttca cgtaccatcg ccgcagcttt
    23281 tgacggtaaa tcggaatgcg ggcgcgcctc attaacgacg cgggctgaat cggcataacg
    23341 cagccagtgg gagacggcag gcatatggtc catgcactga cagctggcaa tggcggtcat
    23401 cgcgccacag ttggaatgac cacaaatcac aatgtcagat acccgaagcg cagcgacggc
    23461 atactccacc gaagcagaaa cgccaccggg ttccggcccg taggaaggga cgatattgcc
    23521 cgcgttgcga ataacgaaca gatcgccagg ctcacgttgc gtcaccagct cagggaccag
    23581 acggctgtcg gagcaggaga taaaaagtgt gcgcgggctt tgctgtgtcg ccagctgttt
    23641 aaacaaggct tcccgcttcg gaaatgcctc gcgctggaat ttaaggaatc catcaataat
    23701 ctctttcacc gttaacctct gtctgtctct ggaatgagag gcagactact cgccgattgt
    23761 cataaggtaa aagtctcatt tatgatgagt tctattggat ttacttatag gttggcgaat
    23821 gctctctcga catatcaatt attttcttgc cgtggctgaa catggcagct tcacccgtgc
    23881 cgccagtgcg ttgcacgtct ctcagcctgc gctttcccag cagattcgcc agttagagga
    23941 gagtttaggc gtgccgctgt ttgaccgtag cgggcgaacg attcgtctca ccgatgcagg
    24001 agaagtctgg cgacagtacg ccagccgggc gttacaggaa ctgggagcgg gtaaacgggc
    24061 gattcatgat gttgccgatc tgacgcgagg atcgctgcgt atcgccgtca cccccacctt
    24121 tacaagctac tttatcggcc ctttaatggc ggatttctat gcgcgctacc ccggcatcac
    24181 gctccagcta caggaaatgt cgcaggagaa aatcgaagat ctgctttgcc gcgacgagct
    24241 ggacgttggg attgccttcg cgcctgtgca ttcgccggag ctggaggcaa ttcctttact
    24301 gacagaaagt ttagcgttag tcgtggcgca acatcatccg ctggctgcct gtgaacaggt
    24361 ggcgttgagt cgcttgcatg atgaaaaact ggtcctgctc agcgcggaat ttgccaccag
    24421 agagcaaatt gaccactact gcgagaaagc ggggctacat ccacaggtgg tcattgaggc
    24481 gaactcaatt agcgcggttc tggagctgat tcgccgcact tccctttcca cattgttacc
    24541 agcagcgatt gccacacaac atgacgggct taaagcgatt tctcttgccc cgccactact
    24601 ggagagaacg gcggttttgt tgcggcggaa aaatagctgg cagacagccg ccgcgaaggc
    24661 atttttgcac atggcgttgg aggaatgtgc ggatgttggc gagaatgaat cacggtagcc
    24721 actgacacag gtcagtggct cttcaacaca gaggaaatta ccgtttgtaa tcgatggctt
    24781 ctggctgctc cagatatacg gtggtttgtg ccggttgtgt actggcaatc accttgccgc
    24841 cacgtaccga ataacgtacc ggaacctgac ggcgcagcgc atcaaatcca ttttcagccg
    24901 gcaggataat caggtttgcg ctgtttccgg cggcaatgcc gtaatcctgc aaattcaatg
    24961 ttctggcact gtggtgggtg attaaattca ggccatcgtt aatctgcccg tagcccatca
    25021 actggcaaac atgcagcccc atatgcagca cttgcagcat attcgccgtt cccagcggat
    25081 accacggatc gaagacatca tcgtgaccaa agcagacgtt aatgccggac tccagcatct
    25141 ctttaacgcg cgtgatgccg cgacgttttg gatacgtatc gaaacgtcct tgcagatgaa
    25201 tattgaccag cgggttggcg acaaagttaa taccggacat tttcagcaag cggaacaggc
    25261 gtgaggtata cgccccgtta taggagtgca ttgccgtggt gtggctggcg gtgactcgcg
    25321 cgcccatgcc ttcacggtgc gccagggcag caacggtttc gacaaagcgc gactgctcgt
    25381 catcgatctc atcacagtga acgtcgatga gacggtcgta tttttgcgcc agggcgaagg
    25441 ttttatgcag cgactccacg ccgtattcac gggtaaattc aaaatgcgga atcgccccca
    25501 ctacatctgc ccctaagcgt aacgcctctt ccagcaacgc ttcaccgttg ggatacgaca
    25561 aaatcccttc ctgagggaag gcgacaattt gcagatcaat ccacggcgcg acttcctgct
    25621 tcacttccag cattgctttc agcgcagtta gcgttgcatc cgaaacatcg acatgggtac
    25681 gcacatgctg aatgccgttg gcaatctgcc atttcagcgt ttgccaggcg cgttgtttca
    25741 catcgtcatg ggttaataac gctttgcgct cggcccagcg ttcaatgcct tcaaacagcg
    25801 tgccggactg attccagttc ggttgtccgg cggtttgcgt ggtgtccagg tgaatatgcg
    25861 gctccacaaa cggcggtata actaaacctt gttcggcatc caggctgttt tcagttatgg
    25921 gcatcacgcc ggattgcgca tcaatggcgc tgatttttcc gtcctgcaga tgaatctgcc
    25981 acagcacctc tttgcctggt aaccgggcgt taataattgt ttgtaaagcg ttattcgaca
    26041 ctgttaacct ccacatgcgt cattgctgct gtcgttttac gattcaaaat cgggttaagg
    26101 atcagatagc tcagcgcgcc acctaatacc gcgttgaccg gaacaattcc cggtaaccag
    26161 tggcctgcgg caatccccag cgcgaccgcc agaatcgcca cccaattgac actcatcata
    26221 cgcgtggtcg caaagtgctc atagcggcga cggttcatca gatagtcggc gatgatcacg
    26281 ccccctactg gaggaatagc tgccgaaagg aaggtcaacc agccgacaaa attgttatac
    26341 agccataatg cgcagaccgt accgataata ccgttgatta ccgaaagggt tttgctcgac
    26401 ataccggtaa tgttggcgaa acctaaaccc gacgcataga gtgcgttatc gttggtggtc
    26461 cagatattca gccccagcac cacaatcgca ggcagcagca ggccctgagc aatcatcaca
    26521 tcagagatat ccgccatgcc cagtgccgca gcgcccgctg caccgaaaat aaacatcaac
    26581 gagttgccga ggaaaaaggc caccatcgcc accagcaccg ccagtttggc attgcgacca
    26641 aaccggacaa agtcagcggt gagcgtaccc gcactgataa atgaccccac aaccagcgcc
    26701 agcgcgacat tgaaatctaa cggttgtgcg ggaacgaccg cttttaatgc gtccaggccg
    26761 cccatgccgt taacagccag ccacacggaa taaccgccca ggcaggcgat agccggaacc
    26821 gcaatcaccg aaagaaccgt cagcgccgaa atgccaaaaa agacggtgac ggtcatcagt
    26881 aaaccggaaa cggcaatcag caaattaata tccagcccgg ttgccttacc caccggaatg
    26941 gcaaacatcg ccacaccgac gccaaaccag ccaacctgag ttccgcccag tagcagtgaa
    27001 ggcagccatg agcctttaac accaaacgag aagcgagcaa gaagatgagt ggtcaggccg
    27061 gtttttgcgc caatgtaacc gagaaatgaa gtgtaaatac cgaggagaag attaccgatg
    27121 agaactgcga ggaagaaatc atgatagcta agaccggttc cgagagtgcc gccggtccac
    27181 atactggcgg aaaagaaggt taatcccagc atgacgaacg tcaatgccaa tacccctttc
    27241 cgcgccgact gcgggactgg cccctggcta aagttgttat cttgcgacac gaaattcccc
    27301 ctttttgctg tttgaaaccc aaaaaatccg ccgtattcta ttcatctgaa gataaaaagc
    27361 aatcgttttc gtggggaaat atatttttta tatgaatgag tgcaaaaagg tgggggaaaa
    27421 attatcggtg taaaaacagt gggtagtggg tagatactga taagacgcgt cagcgtcgca
    27481 tcaggcatcg gcgcggggtg tcggatgcga cgcgagcgcc ttatccgacc tacggttcag
    27541 gcctgtaggc ataataagac gcggtaagcg tcgcatcagg catcggcgca tggtgtcgga
    27601 tgcgccacgc gacgcatccg acaatacctt actcttccat cgcctggcgg atctgatcca
    27661 atgacgcagg atcatcaatg gtcgtcagat ccccaggatc gcgtccttcg caaattgcct
    27721 ggatcgtgcg gcgcagcatt tttccggatc gcgttttcgg caattgcgag acaaaccaga
    27781 cgtgcgccgg gcgaccaaag ttgccaatct ggctgtccac cagcgccata atcgccttct
    27841 cttgcgaatg cgccacatca cgatcttcca gactatcgct ctctttcgga atgacaaacg
    27901 ccaccgccac ctgccctttc agcgcatctt tcaccccaac caccgccact tcggcaacgc
    27961 ccggatggct ggagatactc tcttcaatct cgcgcgtgcc cagacgatgc ccggcaacgt
    28021 taattacatc gtcagtgcgc ccgagaataa agtgataacc gtcagcgtca cggatgcccc
    28081 agtcaaaggt ggcgtacacc gggcgggaaa acagcgacca gtaagtcttc acaaagcggt
    28141 cgtcgtcgcc ccagatggtc tgaatacagc ccggcggcag cggcccttcc accaccagca
    28201 tccctttctc gttgacgcca cacggttcgc cggtgacttc attaagcaac tgcacgttat
    28261 agccatacat cggcacgccg gggcttccca gacgcgtcgg cctgtcgtcc agaccgcgag
    28321 caatcgccat aatcggccag ccggattcgg tctgccagta gttgtcgatg accggcacat
    28381 ccagcgtatt gctcacccaa ctggcggtcg gctcgtccag cggttctcca gccagataga
    28441 gcacttccag cgacgagaga tcgtgtttgc gaatttcagc ggtagggaat tttttcagca
    28501 cgcgaatggc ggtcggcgct gagaacatcc ggctaacctg atatttctcg acgattttcc
    28561 accacacgcc gcagtccggc caggtcggca atccttcgta aacgatagtc gccatccccg
    28621 ccagcagcgg cgcgtaaacg atatacgaat gccccaccac ccagccgata tccgatgcgc
    28681 aaaagaacac gctgcccgct ttgccgccaa aaatggtgtc catcgaggtc gccagcgcca
    28741 ccgcatatcc gccgacgtca cgctgcacgc ctttaggttt gccggtcgtg ccggaagtgt
    28801 agagaatgca ggaggtttcg ttggattcca gccacgccac cggtacccgc gcgccgatgt
    28861 gttgatggcg caacgacgcg aaatcgacat cccgcccgct gacgcgcgcc attttcgcca
    28921 gcccgcgatc caccagcaaa acatggcgtg gctggtgctg cgcctgactt atcgcatcgt
    28981 cgagcaattt tttataggga atgattttgc caccgcgcgc tccggcatca gccgagacaa
    29041 tcagcaccgg tttagcgtca tcaattcgcg ccgccacgct gtgcgaggca aaaccaccaa
    29101 ataccaccga atgaatagca ccaatgcgcg cgcaggccag cagagtaata tgcgcttcgg
    29161 caatcatcgg catatacacc agcacccgat cgccgcgctg cacgcccagt gagcgcaaca
    29221 ttgaggccac cgcgttcact tcgtcatgca gctggcgaaa ggtaaaggtg cgctcttctt
    29281 ctgtttccga agagacggca atcaatgcca gtgcctctgg ctgtttctcc agccagcggt
    29341 cgatagcgtt gtggcacaaa ttggttcggc cttcacaaaa ccaacgggca aacggcggat
    29401 tgctgtggtc gagcgtttgc gtaaagggcg tctgccagtc aatacgccgg gcctgctcgg
    29461 cccagaactg ctccggttcg ttaatcgaac gctgataaaa ttcgctaaaa gacataatgc
    29521 tctcctgttg aacttacgcc ttaccgcagt ttacttaaat gacgtacagg tcgagatact
    29581 cattgaccgg catctgttcc aggcgagttc tgtcgagaga aacctccaga atgcgctgct
    29641 gttggcgagt cgggaactgg cgcgcgagat tgattttgaa tttatcgacc agtttcggaa
    29701 taccatcctg acggcggcga gcatgaccaa tggggtactc caccaccact tcttcaaatc
    29761 gtgtgccgtc ggtgaactca agggtaatgg cattggcgat ggcgcgtttt tccgggtcgt
    29821 ggtagtcagc ggtaaatgcc ggatcttcaa agcaattgat cttctcgcgc agggcgtcga
    29881 tgcgtttatc ttgcgcaacg ttgtcctcgt aatctgccgc cgttaagcgc ccgaataaca
    29941 gcgggatcgc caccatgtac tgaatgcagt ggtcgcggtc agccgggtta ttgagcggcc
    30001 cctttttgtc gatgatgcga atacaggctt cgtgggtgcg aatggtcact ttttcgatat
    30061 ccgccgccgt cttgcctgct gcctgcatct gttcatagag cgtcatcgcc gcttcaactg
    30121 ccgtctggga gtggaactcc gccgggaagg agattttgaa cagcacattc tccatgacgt
    30181 aagaaccgta cggacgctgg aagcggaacg attcaccttt aaaggagacg tcgtagaagc
    30241 cccataccgg cgcggtcagg gctgacgggt aacccatttc gcccgttttc gccatcagtg
    30301 ccagacgtac cgcgcgggaa gtggcatcgc ccgccgccca ggatttacgc gtgccggtgt
    30361 tcggcgcatg gcgataggtg cgcagcgact gaccgtccac ccacgccagc gaaacggcgt
    30421 tgagaatttc ctcgcgggtc aggccgagca tttcggcgac cacggcggtg gaagccactt
    30481 tcactaacag aacgtggtcg aggccgacgc ggttaaagga gttttccagc gcgatgcagc
    30541 cctgaatttc atgggctttg atcattgcgg tcagcacctg tttcatggtc aacggcgctt
    30601 tgccgctggc gacagcgttg cgcgaaagcc agtctgccgt tgccagaatg ccgccgaggt
    30661 tgtcggaagg atgcccccac tccgccgcca gccaggtatc gttgaaatcg agccagcgga
    30721 tcatcgcgcc aatgttaaat gccgcctgga cagggtcgag ctgaaactga gttccgggga
    30781 cgcgcacgcc gttgggtacg acggtgccag gaacaattgg ccccagcagt tttttacagg
    30841 ccgggtattc gagagcttcc agaccgcagc cgagcgtgtc gagcaggcag taatgtgcgg
    30901 tgtcgtaggc cactttagag ctgatttcgt agttcatcac gtaatcgacg atatcaacga
    30961 tttcacgatc aaattccggg cggatgttgt tgatttgagc tgacattggg tatgtttcct
    31021 tattgttatt cgtagaggtt tactggcgct tatccagcgc gacaaactgg cggtcttccg
    31081 gtccaacata attggcggaa ggacggataa ttttgttgtc ctgacgttgt tcaatgatgt
    31141 gcgccgccca gccggtgaca cgggcgatga caaacagtgg tgtgaacatc tcggtgggaa
    31201 cgcccatcat gttgtaggaa acagcagaga accagtcgag attggggaac atctttttgc
    31261 tctcccacat caccgtttcc aggcgattgg cgatgttgta catcttcaac gagccgcctt
    31321 cctgcgagag ctgcttcgcc acacgtttga tcacctggtg gcgcgggtcg gcgatggtat
    31381 aaaccggatg accaaaacca atgaccactt ctttgttttc cacccgcttg cggatatcgg
    31441 cttcggcttc gtccggcgtt tcgtagcgtt gctggatctc cagcgacact tcattcgctc
    31501 cgccgtgttt cggcccgcgc agtgcgccaa tcgcgccaat aatcgcggaa tacatatcag
    31561 agccagtgcc cgcaatcacc cgactggtaa aggtggaggc gttaaactcg tgttcggcgt
    31621 acagcactag cgaaatatgc atcgcctttt cccagctttg cgatggcttt tcgccgtgca
    31681 gcagatgcag gaagtgacca ccgatggagt cgtcatcggt ttccggctgg atgcgttcgc
    31741 cgttgtgact gtagtgatac caataaagga gaatcgagct aagtgatgcc agcagtttgt
    31801 cggcaatatc ccgcgcgcca gagacagtgt gcccctcttt ttctggcagt gtgcagccga
    31861 gcgcggaaac gccggtgcgc atgacatcca tcgggtacga cgccgccggt aaggcttcca
    31921 gcacggtacg cacgttagcc ggtaaaccgc gcagggcttt cagtttcgtt ttgtaagcgg
    31981 cgagttcgtc acgggtcggc agtttgccgt ggatcagcag gtgcgccact tcttcaaatt
    32041 cgcaatgttc cgccagatca agaatatcgt agccacggta atgcaggtca ttgccacttt
    32101 tacccacggt gcagagcgcc gtattgcccg ccggaacgcc agaaagtgcc acagattttt
    32161 tcggtttaat gacatgggta ctgttttgca ggatcgttgt gtcgctcatg ttgtcctcgt
    32221 catttgtagg gtcgagatat ttttcgttgt gttttatttc acctggctac gggcaaacag
    32281 gtcgtcgagc ttcgcttcgt actggtagta gttgatgctt tcgtacagct cgttgcgggt
    32341 ttgcatggta tcgatgacgc ttttctgcgt gccttcctga cgcaggacgt tgtagacatg
    32401 ctcagcggcg cggttcatgg cgcggaacgc tgaaagcggg tacagcgcca ttgcgacatg
    32461 ggcgctgcgt aattcgtcga tggtaaacag cggcgtggca ccaaattcgg taatgttggc
    32521 gaggattggc acctgcaccg catcggcaaa ctggcggtac atggcgagtt cggtaatcgc
    32581 ctccgggaac aacatctcgg cacccgcttc aacataggcc tgcgcacgct cgatcgccgc
    32641 atccagcccc tctaccgcca gagcatcggt gcgcgccatg atcacaaaat caggatcggt
    32701 tttcgcatcc accgccgcgc ggatccgatc caccatctct tctttcgaga cgatcgcttt
    32761 attcggacga tgaccgcagc gtttcgcacc aacctgatct tcaatatgca atcctgccgc
    32821 accggcttta atcatcgatt tcacggtgcg cgccacgtta aaggccgaag aaccaaaacc
    32881 gatatccgca tccaccagca gcggcagcga acaaacgtcg gtgatacggc gaatatcggt
    32941 cagcacgtca tcaagagtag aaataccgag atcgggcagc cccagcgaac ctgccgccac
    33001 gccaccgcca gaaagataaa ttgcctgata tccggcacgc tgcgccaaca gcgcatgatt
    33061 agcgttgatg gtgccaacaa tctgcaatgg attttcttta gtcagtgcag cgcgaaacgc
    33121 tttacctgga gagtgtagag acatagccca tcctttgtta tcaacttgtt atttgcgttg
    33181 ataaagacaa agcaaggggt gtgccacaat tgccttaata aaattaaatg tttaatttca
    33241 atttgttaga gataaagtca tgaactaatc cgcaaatatg cgtttcagtt aacgtttcag
    33301 gcaatgtttc accgcgtttc attgcaacaa ttatgaaaca agactaaacc caatattcgg
    33361 tttcttaact ttgcggtgcg ctatggcaca tccaccacgg cttaatgacg acaaaccggt
    33421 tatctggacg gtatctgtaa cgcgcctgtt cgagctgttt cgcgatatca gcctcgagtt
    33481 tgatcacctg gcgaacatta cccctatcca gcttggcttt gaaaaagcgg tgacctacat
    33541 ccacaagaaa ctggcaaacg aacgctgtga cgccatcatc gcggctggat ctaacggcgc
    33601 gtacctgaaa agccgcctgt cagtgccagt tattttgatt aaaccgagcg gctacgatgt
    33661 gttacaggca ctggcaaaag ccggaaaact cacctcttct atcggcgttg tcacttatca
    33721 ggaaactatt ccggcactgg tggcgtttca aaaaaccttt aatttgcgcc tcgatcaacg
    33781 tagctacatt accgaagaag acgcacgcgg gcagattaac gagctaaaag ctaacggcac
    33841 cgaagcggtg gtcggcgcgg ggctgattac cgatctggca gaagaagccg gaatgaccgg
    33901 aatttttatc tattccgccg ccaccgtgcg ccaggcgttc agcgatgcgc tggatatgac
    33961 gcgcatgtcg ttacgccata acactcacga tgccactcgc aacgccctgc gtactcgtta
    34021 cgtgctgggc gatatgctcg gtcaatcacc acagatggaa caggtgcggc agactatttt
    34081 gctgtatgcc cgctccagtg cggcggtgtt gattgagggg gaaacgggga cgggcaaaga
    34141 actggcagcc caggcaattc atcgggaata ctttgcccgc cacgatgcgc gacagggcaa
    34201 aaagtcgcat ccgtttgttg ccgtcaactg cggggcgatt gccgaatcgc tgctggaagc
    34261 ggagctgttt ggctatgagg aaggggcgtt taccggctcg cgacgcggag gtcgcgccgg
    34321 gctgtttgaa atcgcccacg gtggcacgct gtttttggat gagattggcg aaatgccgct
    34381 acctttgcag acccggctgc tgcgggtgct ggaagaaaaa gaggttaccc gcgtcggcgg
    34441 gcatcagcct gttccggtgg atgtgcgggt cattagcgcc actcactgca agctggaaga
    34501 agatatgcgg caagggcagt ttcgccgtga cctgttttat cggctgagca ttttacgcct
    34561 gcaactgcca ccactgcgtg agcgggtggc ggatattctg ccgctggcgg aaagcttttt
    34621 gaaagtgtct ctggcggcgc tctccgcccc gttttctgcc gcattacgcc tgggattaca
    34681 ggcaagcgaa accgtgctgg tgcactacga ctggccgggc aatattcgtg aactgcgcaa
    34741 tatgatggag cgactggcgc tatttttaag tgtggaaccg acgccggatt taacgccgca
    34801 atttttgcag ctgctactgc cggaactggc gcgcgagtcg gcgaaaactc ccgctccacg
    34861 cttactgaca ccacaacagg cactggagaa atttaatggc gataaaacag cagcggcgaa
    34921 ttatttaggc atcagccgga cgacgttctg gcggcggctg aaaagctgaa ctgccgaatg
    34981 cagcgcgaac gctttatccg gcataaattt caatagatga tcaccgataa acttacgcat
    35041 cgggcatgtt tacatagggt tcagaattac ttcttcttat aaatatttgc cgtgccgtgg
    35101 atcttattgt cagtttgacc ggaggtcagt accaacacat cagccccttt ttcatccgct
    35161 tttttgatca aatcttcttt cgcgtctgca gtcgacattt cattgctagt tgaaatatca
    35221 cctatttttt catactgcga ttcaactttt tcaaattccg ctttggtcat caattcagcg
    35281 gcatagacat tggtaacggc taacgctaac gcaccgacta acattttaga gattattttc
    35341 atattacggc tccagagttg ttatcacact atcatccacc cacattattg ggttgattaa
    35401 taattagttc agtcagggca taaacgccag taaccggaaa taagacactt tgtgatctct
    35461 ctcgcacccg attatttttg gtagaaatat gatgaagtta cttcacttat ttatggatga
    35521 aatcactatg gatcctttgc acgccgttta cctgaccgta ggactgttcg tgattacttt
    35581 ttttaatccg ggagccaatc tctttgtggt agtacaaacc agcctggctt ccggtcgacg
    35641 cgcaggggtg ctgaccgggc tgggcgtggc gctgggcgat gcattttatt ccgggttggg
    35701 tttgtttggt cttgcaacgc taattacgca gtgtgaggag attttttcgc ttatcagaat
    35761 cgtcggcggc gcttatctct tatggtttgc gtggtgcagc atgcgccgcc agtcaacacc
    35821 gcaaatgagc acactacaac aaccgattag cgccccctgg tatgtctttt ttcgccgcgg
    35881 attaattacc gatctctcta acccgcaaac cgttttattt tttatcagta ttttctcagt
    35941 aacattaaat gccgaaacac caacatgggc acgtttaatg gcctgggcgg ggattgtgct
    36001 cgcatcaatt atctggcgag tttttcttag tcaggcgttt tctttgcccg ctgtgcgtcg
    36061 tgcttatggg cgtatgcaac gcgttgccag tcgggttatt ggtgcaatta ttggtgtatt
    36121 cgcgctacgc ctgatttacg aaggggtgac gcagcggtga gcagataatt tactctctac
    36181 gtaatcaacc tgatttgccg aatcccgacc atcggcgatg attgcccccg taatgcgtgg
    36241 tccttatttc cgataagttt acttgagtct ttagcttccc atttcagccc actctcccct
    36301 gtgtgggctt ttttcattgg tctgacccgt cctgaatagc caaacaatgg caatgttcca
    36361 ctttgcaggg atgataataa ggggatctga ttcagaagag ttttgaaaag ttggaccgcc
    36421 atcgaggcct cgaaccccgt accatacaac aatcctggtt gtcgtctgct cttcctgatg
    36481 agctaatggc ggtttttgat atgagcctta cagcatattt tctgtcaggc ttgagtgcga
    36541 tgctaaggta cagaaaagac cccacacaaa catgatggaa ttatgatttt tttcatatga
    36601 atgtcaaccc acacattcac aaaacatctg catgagatca ttttttatac accgcatagc
    36661 tgcgaaacca ctgctggtgc ttttttaagc gaagcgtcgc cgatcacaac aaaatatcgg
    36721 cgaaaaaggc ggtggaatgc catttggcat acggatatac agatgaaatg attacctgct
    36781 ttgcgattca ctctctggtg gaataacaac ttgcaggtgg aaccctttct gaactaaaga
    36841 gtgtagcgga ttttaacgtg cttatttaca ggataatatc tgtcacctaa ttcctgcact
    36901 ctattcgata tccattcatt taatcgtgcg tcatctctgt atgtttttac gtccagaaca
    36961 gagagcaata acatcaccag tgattcagga aaaacagggt ggatattttt taagtgtaat
    37021 aataatccat gcgcaagcgc ctgcttaatg ggcgcgtctg tctccggaat agacgacatt
    37081 tcgcgaagcc atttttccac acgcatacac tctttccctt tagggaaaat tctcagcaaa
    37141 ggcatatggt gttcaagaag aatacatttg caatctgttc gcactgaatc gataaactgc
    37201 actaatttct taagcccggt aaagcgtcca gcctgcacat ttgctttaat atcaagaaac
    37261 gcatcatcct ggtcatatac ccaaactgtc cctttttgat tttcaagtat gcgaaaaaca
    37321 aacttaaccg ggtgatcagg gtaatccatc cagtcataaa atccagggaa atcattaaac
    37381 tgcacattta tggtgagtgc gttatcgtct gaccactcac agtctcgtac ggcagccatg
    37441 agatgaatga gtttgtcgtc atcgactaaa tcatcacgac aatcataagg ataatcataa
    37501 tattgtttaa tcaaaacctt gaatatatcg acacaggaaa ccagggagct tttgatatcc
    37561 tctggtaact gattgttttt aaccgtatta agtatgcttt gttgcgtata tggcatcaaa
    37621 ttattgtgcg gagcttttaa tgatatcatc gcgatctctc ttaaataaaa ataaacgtcg
    37681 cagataataa agctctcatt aaaccaggca ctatcagaaa tcgctcataa ctaacgtgtg
    37741 aagtattgtg tactggaggg agttaattta acaatttgaa tttacgttat aggttttact
    37801 gctactttga tattatttcg ctctccttaa ctaaaaagtt aagtggagag cgaagttaat
    37861 atgttaaacc acagcgtatt tattaatttt ttcagtctgt tagtgtgcga ttatcgataa
    37921 caaaacgata tttcacatca ccacgcagca ttcgctcata ggcttcatta atttgatcgg
    37981 cccgaatcat ctctatatca gccacgatgc catgttcggc gcaaaaatcg agcatctcct
    38041 gagtttctgg aatgccgcca atcatagaac cggctatcgc acggcgtttc atgatcaggt
    38101 tgaaaacttc cggcgattta tgcggtgtcg caggcgcacc aaccagcgtc atggtgccat
    38161 cacgcttcag caaggtggta aaatcgtcga gattatgtgg cgcagctact gtattcaaaa
    38221 tgaaatcgaa actcttcaca tgagccgcca tctcatcggc attgcgtgag ttaacaactt
    38281 catcggcccc cagggctttt gccgcttcgc gttttgcctc agaagtggta aatgccacca
    38341 catgtgcccc catcgcgtgg gccagcttaa tccccatatg tcccaggccg ccgatgccga
    38401 ccacgcccac ttttttaccc ggcccggcct gccagtgacg tagcggcgaa tacgtggtga
    38461 tccctgcaca caacaaagga gccaccgccg ccagctgctc ttgcgggtga cgaatacgca
    38521 gaacatatcg ctcatgaacg acgatctgtt gtgagtagcc gcccagagta tggcccggtt
    38581 cgtccggcgt cggcgagtta taggtgccgg tcatgtgatc acagtagttt tccagcccgt
    38641 cttcacactc ttcgcaatgt ttacaactgt cgacaatgca gccgacaccg accagatcgc
    38701 ccggcgcatg tttttctacc tgatcaccaa cggctaccac acgccccaca atttcatgac
    38761 ccggtacgca ggggtaaacc gtccccgccc actcggaacg gacctggtgg agatcggaat
    38821 ggcagacgcc acagtaagcg atttcgattt tgacatcatt cggtcccggt tcacgccgtg
    38881 tgatatccat cggttcaagt ggttgtttag cggaatatgc accaacagct ttgatcttca
    38941 ttgtgtttac tcctgattag ctatgtgtat ttggcgatgc ctgatatgtc atcgcggcaa
    39001 aacgcgtcca ttgaatatag ccaatattta aaccacccga tccagcccca acatgccgcg
    39061 aacctgaatg aaacagagca gcaacgttcc ccataaggcc atcgtcaggt aggggcttat
    39121 accgagaata ttgaagccgc tttcaataat ttgcagaaga aacagcgcca gcaccatacc
    39181 aattatccgc ccgctgccgc cgtccggatt aaccccgccg agtactgccg cgaggattga
    39241 caccagcaag tacgattcgc cataagaggc cttagccgag ttgagtttcg acatcatcaa
    39301 aaatgccgct accgcacaaa gcaaggcgga aatgacataa acccaaatca gcacccgacg
    39361 agtgttaatg ccggagtaaa gtgttgctcg ttcgttagac ccaatcaggt aaatggcttt
    39421 accgagcggc gttttctcca gcaatatcca cagccccagt gccactgcgg caaacaacca
    39481 catcggtagc ggaataccga accattgcgc atgattcaac cacagcaccc atgagggata
    39541 attggcaatg gcgctaccac ctgtcaccag aatatttatc cctttgagca acgtcatcat
    39601 cccaagcgtt gcaagaattg gcgacactcg aataccagca atcaatacgc cgttacataa
    39661 accgattatc accgccgcgc cagcgcccgc cagtaacgta gcgacaaccg tagcaatacc
    39721 cggtggatac tgagtcgcca cccacgccat caccagtgaa caggcgttag cagtggctat
    39781 aattgacaag ttaatgccac cgcaaagcat ggtcaccgcc atagccaacg ccagaatgcc
    39841 cagcacgggc atttgcgaag cgacggactg gaaattactt atcgaccaga aaatctccgg
    39901 catagtaatg ctgaacgcgg caacagttag caccagcagg ccgatcaagt aaaactcaac
    39961 gttattacgc cacgattttt tcatagcaga ctccggttct gatgctgact ccacgccgtg
    40021 gcactaatgc tggcaacgat gatgatgccg gtgatcaatg tttgccagta agacgagact
    40081 cccaataaat ttagcccgtt ttgcatcact gccaacaaca ccacgccgag caaagtaccg
    40141 gttaacgtgc cgcgcccgcc gagcaaacta gtgccgccaa gcaccaccgc agccagtact
    40201 gtcagctcat aacccagaag agaatcgggg gcgacagtca tcaccgtcca cgactgcact
    40261 acacccgcag cgccagacat caatcccata tagccgtaga cgaaaagttg cagtttcagc
    40321 acgctaaagc cgatgcgact ggctgattcg cggttcccgc caagggcata aattttgcgc
    40381 cctacactgg tgaaattcat gataaacgct gtcagcagca ccaccgtaat catggcgatc
    40441 agcggcagac caaggccata gtcatagcca tcagcatcgg tgtacttgaa caacataacc
    40501 ccctgctcaa accacggcgg aaagtcgtac agccacacac ctttactcaa ccataacagc
    40561 aggccataga aaatattcag cgtcgagata gtgatgatga tagaaggcac ccgtaggcga
    40621 ttaaccagta aggcgttaat taagccaagt aaaatgccga tgcagcccgc cagtgcaaac
    40681 gcgacagcga agttgccacc aattttttgc agcaacagca ccatgccgta ttgcgcaatg
    40741 atggtcattg ctggaaacga aatatcaatt ccaccggaaa tcaacaccac aaacaaacca
    40801 caggcgagaa tagtcaacat ggcatagtta ttggcgagat cgtacaaatt accgaatgtc
    40861 agaaactcgt cgctgcgcca ggcaaggcca acaaacagca ccacaatcag taatgccagc
    40921 cagaattcat ggcgtttttt cagttcagcc atttaccacc tccgcaatcc tggcctgaga
    40981 actgctgtca ggcaggaagc tatgggtgat ctgacctttt tgcattacca aaatccggtg
    41041 gctttgatac cacgcttctt caatttcatc gcagatcatc aataccgcaa tgccatgagc
    41101 cgccagatcg ctgataatgc cgtatattcc tgctttgttg gcgatatcaa caccgactgt
    41161 cgggctatcg agaatcaaca gtcgtggccc aatcgccagc catttggcga tcgatactcg
    41221 ctgcgcgtta ccaccagaaa gcgtattcac cggaagatga ggatcagaga ctttgatggt
    41281 caatgccttg attagcctgt ccaccaggtc acgaacttta gtttcactca gaaagccgaa
    41341 gcgatctttt accttgtgaa aaacagtgct aatgatgttg tcttcaatag attgcgccat
    41401 caccaaaccg cgcgacatgc gatcttccga aacataacca ataccgtggc ggatagcgtc
    41461 ctggttacta tgcagtgtaa ccggctggcc gttgatgagg atctcgccag catccggtcg
    41521 ggtcatacca aacagactca gacataattc cgtgcgcccc gcaccaagca ggccaacaat
    41581 cgataccact tcgcccgctt ccacccgcaa gttaatattt agatattccc catgacgact
    41641 taaattacgc acctcaagca ccggggtgct ggattttccc tgccacaact ctcgcacctg
    41701 atattcgaac tcctggccgg tcatcaggaa accgagttgt tttgtggtca tctctgcagc
    41761 cggaaaagtc ccgaccagct caccatcttt cagcactgaa atacggtcgg aaacttccat
    41821 cacttcttcc agacgatgac tgacgaagac cacgcagatc ccgcgttccc gcaattgatg
    41881 caccacctgt aataaccctt gcacttcctg atgggtcagc gaagcggttg gttcatccat
    41941 cacgatcaac cgtgcgtcct gcgccagtgc acggcaaatt gctaccagct gacagcgggc
    42001 aattgaaagc tcagaaacga gcgtatccag tggcaatgtg acgttaatac tggtcattgc
    42061 cgcctgcgcc acctcacgca gacgacggcg gttaacaaac agaccgtggt gatagtgatt
    42121 cacggcgata ttctcccaga cactcaggtt aggaaataaa gagaggtcct gataaatcac
    42181 ctgaatcccc tgcgccaccg aagcggcggg tgtcagcttt gaccagtttt tcccaccaag
    42241 cgtaatttcc gctccctcat ccgggcgata aacaccggac ataattttaa tcaacgtcga
    42301 tttaccacag ccgttttgcc cggcaaggca gtgcacttca cccgacataa agtcgagaga
    42361 aacatcgcgc agcgctcgcg tggcatgaaa cgttttgttg atatgacgaa gggaaaggaa
    42421 ggtttccata acatcccctg gcaataagaa tcggggcttt cgccccgaag aaatggatta
    42481 ataaagtgag tcgatattgt ctttgttgac cagtaatacc ttatggaaac ggataattcg
    42541 cttatccata tctacgtcgg ccttacccag attctgcatt tctaatccag gtttgatctc
    42601 ttcacccttc agcaacgtgc tggcgacggc tgcgagtgcg taacctgcag aggccgaatc
    42661 gtaagtaatc ccttcggtga tatcaccact tttaatcagt gatgccgcct gtgaagggat
    42721 catcatgcca tagactgcga ctttattttt cgcccgtttc tctttcaccg cacgtcccgc
    42781 gccaatcgga ccgtttgaac caaaggagac aaccgctttc aagtcaggat aggttttcat
    42841 caggtccagt gtggtacgac gtgagtcatc cacactctcg gcaaccggca tgcggcgggt
    42901 aacttcatgc atatccgggt aatgctcttt ctggtatttc accagcaagt cagcccataa
    42961 gttatgctgc ggcacggtca aactacccac gtaaatcaca tagccgccct tgccccccat
    43021 gcgtttcgcc atatgctcaa catattcagc ggcaaatttt tcgttatcaa tgatttcgat
    43081 atcccagtta gcacttggct gaccggggga ttcgttggtc agaaccacaa ttccggcatc
    43141 tcgcgctttt ttgaataccg gttccagcac gttggcatcg tttggcacga tagtaattgc
    43201 attaacctta cgggcgatta aatcctcaat aattttaact tgttgcggag catcagtact
    43261 tgaaggcccc acctgtgacg cattaacacc aaaggcgtta cccgcctcaa ccacaccttc
    43321 gcccatgcga ttaaaccacg gcataccatc gactttagag atattcacca cgactttttc
    43381 cgctgcctgg agcggcgcag aaattagcgc agcgcctaat aacagcgaag acaccatatt
    43441 gataacaaaa cgtttattca tcatttcagc ctcattgcag ggtagtcagg aagtgttcgc
    43501 ccaaattagc gcgctggaga gaataagaat tttcgaatat ttttatgcag aataaacagc
    43561 cctatttcag gcagctatta aaaatatggt tataaatatg gacaaaacgg gcagcaacaa
    43621 taagtgtgac attcatcacg taaaaaatag caaaataaac aaaattagcg atattttatg
    43681 tgtgtgagtt tatttcgcgt agataaatag ttatctacac aacgtcaata agggcatatt
    43741 cttcaaatat aacgtccatt tctggtgaat agcttccttc tgcctggaaa aaacgacggt
    43801 gttcattacg ccaatattca agactgaggt caccttcacc ttccttacgg gcaagctcca
    43861 cggtgacatc agaaaagcgc agtaaatgca ggccaatcac tctgataaca catacaggct
    43921 caccacggct attttctaca atcttatact caccaatcat cggaaatgcg ttgtcttcaa
    43981 tacatcccgc cagcgaacca caggaagctg ttttaacacc agtcgcaatc aacgttgcga
    44041 ggtgatcggc caacgctgct gaatcgccaa aagtacaaaa ggaagcatgg ggatattttt
    44101 gtgagaattt ttcttttatg gtcatggcaa tatttttgcg attacggaaa aacaaatcac
    44161 atcttaatta ctgttggtaa ccatcagcaa gagtatcatt ggtttttcga tactgaatct
    44221 tttttatccg aagcaggtca catttatggc ctgataaata cctttgttaa aactcacttt
    44281 tgtgtatttc tctcaaataa tcccctggaa attatgccgc gagattataa agttcaggat
    44341 cattcattag caaaccattc ccgccccact ccctggggct taaatctctt acaattcacc
    44401 gttgcaggcg tttcaaatgc gcatcaacct gccacactcc cccacaccag ttgcccttta
    44461 tggaacgttg cggttctcgg cgagatacgc gccaccgcct cggcggaaca ggaggcgtcc
    44521 accagcacaa agctggcatc atcctgcgct tttggccata cacgctcgcc tttttcattc
    44581 agcggcaata catcgccagt ggctagaaac agcgaacggg agaggttctg ttcgttagga
    44641 cgaatataga gctgcgcgta gagattggct ttttccagca tgtcgcccag accataaggc
    44701 gaccagtggt cgataacgct gtcagtgcca gtcatcactt ttacgccttt gtcgtgcaac
    44761 tgtttgagcg gcatatgcag cgtgccaatc ggcaccgtcg aggcgataga aatttgctgc
    44821 gccgccatcc gattcgccag cgcatcgacc tgttgttcgt tgagcgtagc cagcgcaaag
    44881 gcgtggctga tggtcagctt acctttcagt tgcggcgttt tctctaccgt ttcaaccata
    44941 taattgatgg ctgccacgcc cgacggagtg gtttcgtgca ggtgaatatc gacacctttg
    45001 tcgtagtcca gcgcaatctg aaacatggtg tcgagggatt tttccatcgc gccatcgaca
    45061 ctggtcgggt ccagcccgcc gacgtaatgc gcacccgcct gcatcgcttc gcgcattaag
    45121 ggttccgatt tcgacagcag caaaccgtgc tgcgggaagg cgacgatttc acactcgaag
    45181 cccgcctgac gtcgcgccag caccgcctgc aaattctgca gatttttcag gccggaaacc
    45241 ggttcgatat tgcagtggct gcgggcaatg gtggtgcctt tcgactgcaa tagatcgatc
    45301 agtttttctg cccgttcctg cgtgtacggt tgcagttccg gcagcatttt ctgctcaagc
    45361 ttgatcatgt cctggatagt ggtgcctgcc ggacgattga gcgagcgcca cggcccgccg
    45421 tagaaggttt tgtcgagatg aatatgcatg tcgcgagtgg tgggtagcat caacttaccg
    45481 ccagcgtcat agtgcggcag cgtggcgtcc ggatgctgct tgttctcgcg cagggcgaca
    45541 attttgccgt cctgaatctc cacggtctgg cgcgccgtgc gggtctgcac cgccacaccg
    45601 ttttcgtagt caaaaccggt ttccagcagc acgttatcga gatagtaatg cgggtcagtg
    45661 attttcatgg tgttgttcgc ttcgcagttt agggtgccag ctaccgccgc atgggcgagc
    45721 ggcacagagg taccaaacag cgcggcggcg gtgaccatct taccgctctg gctcagaaat
    45781 tcacggcggc tattgctttc tttcatccta cgtccttacg ttttaataat atgggtgccc
    45841 gtttcaccgc gcagcgccgc aggcaggcat tccggcgtgg tgataatcac ttctttgccg
    45901 ccctgctcca ggaacgtcag gctggcgatg atttttggca acatgctgcc gggcggaaaa
    45961 tgcccttcct gcatatagcg agtcatggtg gcaatatcca cccgatccag cgcctgctgc
    46021 tgcggtttac caaagtggat acagactttt tcgacgccgg tggtgatcac cagaatgtcg
    46081 gcgtgaattt cacgggccag cagcgcggta gagagatctt tgtcaataac cgcgtccacg
    46141 ctttggtaat cccccgcttc agtacgcact accggaattc caccgccgcc cgcgccaatt
    46201 acgacaaaac cttgttggat cagcgcctta atggcgggtg cttcgacaat acgtttcggt
    46261 tccggcgagg cgaccacgcg gcgatagccc cgtccggcat cttcaacaaa acaccagtca
    46321 gggtttgcct tttgtagttt gtcacgctgg ctttcactga agaatgcacc aatgggcttg
    46381 gtgggatggg caaaacctgg atcgttttta tccacttcca cctgagttac cacggtgacg
    46441 gcttttttct cgccgtgacg cgccagccgg ttattcagcg cctgttggat cagatagccg
    46501 atgccgccct gcgtatctgc cacacagttc gccagcggcg ttaagggcag cccttcgcgc
    46561 tcgtgggcaa tctccgcacg gcgtaaatcc agcccgacct gcggcccgtt accgtgggtc
    46621 agcacaatgt cgtaatcgga agccagcatt tccaacaccg tatcggccac ggctttcacc
    46681 gcctccgcct gatgctcaat cgactggctg gcgttatctt tgataatgct gttgccacca
    46741 atggcaacga ccacaagctc tttcatgttg ttatgtccgc tacagagggt gattaggtta
    46801 tgaccaacgc cagactgcct gatgcgctac gcttatcagg cctacaattc tcgggccatc
    46861 cgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa catgtaggtc agacgccgta
    46921 acgttcgcac cagccaagaa tggctttctc gaagcaggca aaaggtggat gcacaatgcc
    46981 cgcgccaatc atgccgacgc ccgcatcttt atgggcgatg gcagtgttga tcaccggcag
    47041 aatgccgctg ctacccacgc gggtgatgtc gattgccgac ggcacgccca taaaaccgag
    47101 cagcggaatg gtgacgttgg ggttttcacc gagagtgatt tcacgcatct ggcgagagaa
    47161 atcgatagcc tcttccaccg tgccgcccac cagcgcaacg attgcaggcg cggtcgccat
    47221 agcaaatccg ccaataccgt aggtttcagt gatggcgctg tcgccgatat ccagccccga
    47281 atcttccggc ttataaccgg caaacatcgg gccgatgacc tgctgcgccg gaccggtaaa
    47341 ccattgcccc ggtaatccgc tgacccgcag gccgaactcg acgccgttac gcgccatggt
    47401 ggtgaccacg gtgctgtatt cgatgccgtg cgccgcatcc atcgccgctt tacacatcgc
    47461 catccacgtc gggccggaga agtagtcgct gctagcgaca aactcaaaca cttcgcgctg
    47521 tttctccacg gaataacctg cctgaataat tcccggcgtc agcgcctgaa tcagcaacgt
    47581 cgtcccggcg ttattgcggt tatggcactc atcgcccata tgcagcgcct gcgccagcat
    47641 taagcgcaga tcaatctcgc cgataatttt catcgcgtcg cgcagcattg gcccctgcac
    47701 atcgcgcatc cagttgaggc ggtcgatcac gctctgatcg ttggctccca tacgcaaaat
    47761 cttcgccatc tgctcgctca tattggtgta agcgatgttg ccgtaggttt tgttttttac
    47821 gatgtgcata aacatcgagg cagaggtaac acccgccatc gatcccacac agtcgtgttc
    47881 gtggcacggc gagaaggtga tctccccgga ggccgccagt tcagccgcct cgtcgagatc
    47941 tttcgccagc ccttcgaaca ccaacgcccc ggtgaccgcg cctttcatcg cgccgcacat
    48001 tttttcccag gtgatcggcg gcccggcgtg aagaatggtt ttcggcgtca tgcccggcac
    48061 cacgttaatc gcctgatcaa aaccaatcag caccggatgc gactggataa tgcgctccag
    48121 cgcctgctgg ttagcggcgg cgattttgtc tgccagcggc gaatcggcaa tgttatccag
    48181 cgcctgcacc acctgcatat tgccctgccc cggcggcgtc cagtcgagtt gagtcacttc
    48241 tacatgctgc tttttcaggt catcgctaaa catggcgatg ccgacgttaa taacgttcaa
    48301 tggttggcta aacagtgact ggctcatcag gcttcctccc ctttgcagat aaattcacgc
    48361 gccagcaatc cggtattggt gctgctgctc gccagaatca ctccggcatc aagcagcatc
    48421 tggctttgtt gttctaacga tggcgtatca agatcggtac ccagcacata ggcgagaatg
    48481 atcaactcgc gtccttcggc agcggcgatc gctttcgctt ctttgatcgc ctcgatggtg
    48541 gagccgaccg gatcttcatg cgatccaaat ccgagcacaa aatccatcac gatcaccgcc
    48601 acttctggat cgcgcgcctc ttcgatcaag cgactgatgc ggttggtggg gtcaatcatt
    48661 ggatgcggct tgccgttcgt gaagtcgtca tcgccaaagt cgaggaaggt gtgtttgatg
    48721 ctgcggttga tatcttgcag gcggaattcc ggatcgggct gaatgttgct gtagacatcg
    48781 ccatgttttt ccatcaccgc gaacatggtt tcgtcgcaca gcgtgccgcc gcagaacagg
    48841 ccacgaatgt atttctgctg cggttgcaga cgcgcacgca catcagcaat caacggctgg
    48901 ttaagcgtat gcaggtcgag attttcctgt ttcacgccgg agagcatcac cgcttttagc
    48961 gccgcctctt tgctgccgcg ggcaaactgt agcccctgct catccaccgg cgtttcaacg
    49021 cgaccgagga agcagaccac caccggcttg cggcaggcac gtgcgcgttc cagcactttg
    49081 cgggccaccg caggcgcggg aggttttgaa atcagtgcga taatttcagt ttgcggatcg
    49141 ttttccagca tcccgatggc gtcgagcatc atcaggccgc cgattttctc gctcaggtcg
    49201 cgcccgccgg tgccaatcag ttgtgaaaca ccgccaccaa attcatgaat gcggacgctc
    49261 agctcctggc tgccggtgcc ggatgcgcca acaataccga tattgccgcg acgcacggcg
    49321 ttaccaaaac agagcgccgc gccgttaata atcgccgtgc cgcagtccgg ccccatcatc
    49381 agcagccctt tttcgtgggc caactgcttg agcgccagtt catcttcaac tgagacgtta
    49441 tcggaaaaca gcatcacgtt gagatcgttt tgcagcgcct gacgcgcttc gcgagcggca
    49501 aacagaccgt tgaccgaaat caccgccagg ttactttccg ggatatgctt tttggcgctg
    49561 gcaatagtgg cgtaacgcgc ctcgtgcgag ccgctttgcg ctttggtgtt gaacagttct
    49621 tcaatctcca ccagcaactg ctcgttgtcc gcacccgatt taccattgat gacaatcatc
    49681 aggtcgccgt ttttcgcctg ttccagctcc ggcgtcagca gtcctaaatt tttcagtaca
    49741 cctttgttca tttcggtcgc catcgccaca aatgcctgct cgacgccgtc gagtttattt
    49801 gcacgcgtgg agatagacat cagcgagaca gaatcaaaat aggtattcgg tttaatgact
    49861 attttaactg acataattcc ctccgtaggt ttggctcagc gcacaaccat cggccatgcc
    49921 atacagcatg tcgcagccgg aactggagcc aatatgttta attttttcta ttgcctgagt
    49981 tgcgttccca gggatgtcat aaataatgtt gtgaataaaa cgatgaatat tttcccggca
    50041 acgttgttgt aatgcggctt ccagcgttat agcacttaat aatgtggtgt tatttctgcc
    50101 gcgctgcata ccgagataaa attcctcttt gtatttttcc gacggatgcc cgggaataaa
    50161 taaaataagc gccagacctg ttaaataatc gtcggctgat ggcgtaaggc caatccctaa
    50221 cccaatcata ctggcgaccg ctgcggcgat attttgtttt tcgtttactg cctgaataac
    50281 tgcccgtcgt ttaatatgta gttctttatt taacgcctga taaaacggat tatcgccgcg
    50341 ataattaaac agggtttcat tttcccccag gtgctgatga ataatgtcgt gccactgtag
    50401 ccaggcgata aggtgaaaat tcacagcggt catttgcaag gttggggaca gccaacgccg
    50461 acaacgactc atctctatat gaagatgttg accaaccgta ataccttgat cgtcaaactg
    50521 aaccttatca ccatgccgga acagatcatc aaagtgagtg agtgccaacc gacaactgtt
    50581 tggcgcattg tcgtactctt cacaaagcag cgtcagcaac tgctggcgag ccgggagtgc
    50641 gatatttacc gctctggcaa agacctgttc aacctgtcct tgcccccgct gcgccagaaa
    50701 atccgcatcc gcagttagcg cccacacaca ccgtctttct ggtgtgttga tagcaagcct
    50761 cccggtttat gcacctgcgg caatcagtaa ctgcgcaatc tcttcaaagc cctgttcccg
    50821 cgccagttcc agtggcgttt tgccatattt atcggtcaga tgcgggctgg caccgtgttc
    50881 cagcaataac tgcacaatcg cctgctgttt aataccacca tcattaagca caatcgcttc
    50941 cagtagcggc gtccagccta catggttggt ctggttaacg ttaatctccg tatgcgccaa
    51001 aagctctttt acaatactta aatggccttt ttcacaggca ggcgtcaggc cgacaccgcc
    51061 aaaacgggtt acgcaattaa gatcgggttt agccggtaaa ataattcgta gtagcgttaa
    51121 atcatcgttc agacagctaa ttaaaaaagg atttaaacag gtatgatctt gcttattaat
    51181 atccgctccg gcatcaatta atgcctgaac gcaagcatat tgctgatata aacttgccag
    51241 cgtaattgcc gttttcccct gacgatcgca ggtattaata tcgacaccca gcgcaaggca
    51301 ggtttttact ttatcgatat caccctgttg tgcagccaat aaatagtcgg caggtagatt
    51361 ttttatactc attcacattc ccctggtttt tgtggctgtt ttcgatgaat tgagaatagc
    51421 aacgataatc tgataaaaga acccgcttaa atttaattcg ctgtatttcg cctttttgtt
    51481 gaatgatgtt caacaatctt ttcgcagtgt gcggtagtgc aatatttgtt tctttgaccc
    51541 gatacccttt cactcaatca ggttttattt ctctatagtc tgcacatgta agagtagctt
    51601 tcgtccatgt ctaaccagag gggaacaaga tgaacggact cacggcaacg ggtgtcaccg
    51661 tcggtatttg tgcagggctt tggcagttag tgtcttcaca tgtcgggctc tctcagggat
    51721 gggaattatt aggaaccatt ggcttcgtcg ccttttgcag tttttatgcc gcaggcggcg
    51781 gtaaatcggg gtttatcaga agtctggcgg ttaattattc cgggatggtg tgggcatttt
    51841 tcgccgcgct ggctgccggt tggctggcct ctgtaagtgg actgtccgcg ttctgggcga
    51901 gcgtaattac taccgtaccc ttctcggcgg tagtggtctg gcaagggcgt ttctggttac
    51961 tgtcctttat tcccggtggc tttctcggca tgacgctgtt ttttgccagc gggatgaact
    52021 ggacggtgac gttactgggt tttctggcgg gtaactgcgt ggggataatt tcagaatatg
    52081 gtggacaaaa attaagcgaa gcgacgacta aacgcgacgg gtattaaaag ttattcaaaa
    52141 atgattatct atctttaacc gatacctcta cagtcatagc tggagaaatt catagtagat
    52201 aatggcgaga atggtttaat gaactcaatt tttaccgaag agaatctgtt ggcatttact
    52261 accgctgcgc gttttggcag cttcagtaaa gccgcagaag agttgggttt aaccacttcc
    52321 gccattagct acaccatcaa gcgtatggag acggggctgg atgtggtgct gttcactcgc
    52381 agtacccgca gcattgagtt aacggagtcc gggcgttatt ttttccgcaa agctaccgac
    52441 ctgctgaatg atttttatgc catcaaacgc agtatcgata ctatttcgca gggcatagaa
    52501 gcgcgtgtgc gcatttgtat taatcagctg ctttatacgc ccaaacacac cgcacgcttg
    52561 ttacaggtgc tgaaaaagca gtttcctacc tgccagatta ccgtcaccac cgaagtgtat
    52621 aacggcgtct gggatgcgat tattaataat caggccaata tcgccatcgg cgcacccgat
    52681 acactactgg acggcggcgg tattgattac actgaaattg gcgcgattcg ctgggctttt
    52741 gctatcgccc cggatcaccc gttagccttt gtgccggaac ccatcgccga aagccaactg
    52801 cgtctctacc ctaatattat ggtggaggac accgcgcata cgattaataa aaaagtgggc
    52861 tggttgctgc acgggcagga gtcaattctg gtgccagatt tcaacaccaa atgtcagtgc
    52921 cagattttag gtgaaggaat tggttttttg ccggattaca tggtccgtga ggcgatgacg
    52981 caatccctgc tggttacccg acaaatccat aatccgcgcc aggattcgcg catgttactg
    53041 gcgactcagc attctgctac gggtcaggtg acgcagtgga ttaaaaaaca atttgcgcca
    53101 aatggcatat taacggggat ctatcaggat ttactgcatc gggagaatta atcgctgcca
    53161 acctcccgat gcgctgtgaa tagcggttta catcaaccac ctgctttcat tacccattca
    53221 gagataaatt tattacccgg tactggcgga gagtacaaat agccctgcaa aaagtgaacg
    53281 cctttaccga tcattaaatc cgcctgctcc tgggtttcta ccccttccgc cacgatactc
    53341 agaccaggct tacgcgctag ttcgacaata ttgtccacaa tatgaccgga gatttcgtcg
    53401 acactcgcca tttgcacaaa tgacttatcg atcttaataa aatcgaccgg gaacgcctgc
    53461 aagtaacgat aggtcgcata acccgtacca aagtcatcca gcgcaaaggt aatgttgtgc
    53521 tgatgaaggc tgtcaaatat cgctctggct tctggcgtta ccggaatagg gttacgttcc
    53581 gttagctcga gaaccagctt gattttatcg ttacctaatt tcttaaccag gttcagacac
    53641 tctttttcaa atcccgctgc caaaaaacaa cccgccgaga cgttgatgcc aatatggaaa
    53701 ttgtccggca gcaaatgttt taccggcatc agaatatccg cagtctgttt catcagttgg
    53761 cgggtcatta tgacaataag accggatgac tccgccagag gaataaactg atccggtggg
    53821 ataattcccg tttgtggatg ttcccagcgg acaagtacct cacagcccgt cagtacccca
    53881 gtctgcgcgc agaaaaccgg ttggatccac ggtttgaatt cgtggttttc caacgccaga
    53941 ctgatggctt caggcgtgac gatatggtga caacggttat cagggatata aaaattattt
    54001 ttatttcccg tgccactgat gacttgcgga tgatattgaa aaaagatatc ccgccagaag
    54061 gtaatatcac tctggatgcc tagtttttca tacagctgct tcttttgatg tgaaattgtc
    54121 tttgcactgc gatttctgta ctgtgagatt tctgtaaccg acattccctc cattagcagc
    54181 ttgagtaaaa gccgttcttt ttcggacacg ataccaggca acgtcaaatt caccgttgcc
    54241 ccgaactctt ccaaaaaaac actcaaatca tgcgaagcca ttgaacacac cttgattaaa
    54301 tcttgtgcgc cagctaaatc tgcgagtctt cactctgact gataaagctg gctgcaagaa
    54361 aatgacacag agagccgaat tatctcccgt tcggctctct ctccttttac tcattattca
    54421 gatcagaatt gccacttaat gccaaccatt gcagaggtgt cattatatcc cctatcggca
    54481 acctgaacgc ccacattccc ccagacattc aggttggcat ttagctgtcc ttccaccccg
    54541 gtttttatct cagcaatatt tcgggctcca tcctgagtga cggacacgcc atccatactg
    54601 gtgctgaaat ccttactgtt atgcagccag ttcacttcta caaacggctg gaactcgcgg
    54661 gatttaccgt catccatttt atggtggctc ttcagccagg tttttacgcc aagtcgggtt
    54721 tgaacattgc catcaccgtt gctatgaacg agggttccgt tgctttcgcg gtgcttatcg
    54781 gctttgactc ccatccaggt aacctgtgct tgcggctgaa cataccattc attacgcgtt
    54841 ccctggctgc cattaaattc agctaatttg tgtttgtatc cagcttccag tgaagcggta
    54901 aatccttttg atttatagga ttcactttgt aagtcatccc ctttcactgt gttatcaaac
    54961 cagctgtact gcgcccaact gtcgagatac gcgccattac gcgactcgtc atcggcatac
    55021 caggtggcat agaggccggt gctatatccg ttcacactcg cttttgcacg ataaccggtt
    55081 cgcgaggaaa tggttttgct gtcgctgttg ccatatcccg ccatgacccc aacatgccag
    55141 ctgtcgctgc cgttttggct ccactgcgcg acatcgcctc ccagttgcag aacatagcga
    55201 ttgctttggg ttttcagctg gccgctgcca tcacgccatt tattatgacc accttcatgg
    55261 cgcatccaca tagtggtttg tttctgctca cccgtcacca tgtcggtata gtacgtatta
    55321 cccagacgct catgcagacg cgtggtgaac attgtattcg ctgctgcaag gttcgcaatg
    55381 tagctacccg cctccggtcg caggtcatta tccacattca gatccggacc cggcgtaggt
    55441 gtcgggttag ggttcggctc tggattcggg tttggctccg gattcggcat cgggtctggc
    55501 tccggctgca gttcaggaga atcactgccg ctggtcagat accagttacc actatttgcc
    55561 ccttgtccac gcgcgagagt gtagtcatac gcccccgcaa cgatacgacc agcctgaaca
    55621 aattcgccct cagacttacc gtctacatgg ataagttcga taccgttaag ggttttcgca
    55681 cctgtaccgc cagcgttatt caccgtaacg gcagtcgtgc cgctagtatc gccctcgaca
    55741 accaatttat cggttactga gtcatcgcca ttcagtaccg tattcattac tagttgaccg
    55801 ttattaccgt ggtagttccc cttcaccact aacacattgc cagcttgctg tcctgacatt
    55861 ccggtcatca gcgtaccgtt gttggtcaga ttgccgccca gggtgtaatt cacagacgca
    55921 gccgctcttt tttcagtgct attattcaac gccagggtac cgttgttctc aacgtttgcg
    55981 ctacccaatc cctgcgcatg acttaccagg acattagccg aattagcaat gttcacattc
    56041 gccgtcaggt tgctgttatc gcccgcgatg tccagcgtgc cgccctgaat attcagctct
    56101 ccgctccccg ttaacgaacc ggttgaagtg cccccgttgg taaccgtcag actgccgcca
    56161 tttaagctca gcagtgaatc ggcgctgcca ttgagcgtgc ccacggtctg gctgtgacta
    56221 ttcatatcca gttcagtctc tgccgccaga cgcagttcat gggtattacc caacacgtta
    56281 tcgttattca acaaaagcgt cccactgcgc agatcggtaa caccggtata gtcgttgtct
    56341 ttgttagaaa gcgatacggt ctgacccttc tggctgctga atgccaggtc accactgccg
    56401 gtaatctttg cgctgagatc ggcggcattc tcgcttttac cgttagagct cagcaccaga
    56461 gcatcgctgt cggtagcgtg taaatcaagc tgggtcaggc cgtaaccgat atacagaccg
    56521 tcattgttta ccccactcgt cagacgatag tcgtaagtgc cgacggccgc tttttgccca
    56581 ttctgctgaa tatcacgttg ggcgctgctg gagaggattt gcccattttt atcctgcaat
    56641 tgcagattgc ccgcatcgcc cagaacttcc gcaccttgcg catcaaccag cttaatggcg
    56701 ctgttaccat catcgacttc agtgagcgat aacgcagagt caatatcacg agatactgag
    56761 cggacaacgt cactgtcaga aacctgaatc acaccctcgc cgcgcaaatc cagcgtttta
    56821 ctaacgttga ccgtcccttc tgtcatctgt gcacctgctg tcagggcacc aaaatcaacg
    56881 gttccgccgg agaatcccaa agtgctaatg gagtgagcgc ccttacccac tgttacctgg
    56941 cttccctgac ccgcagtgat cccgcctctc tgcagaaact cagcattctt ttccaggtta
    57001 aatgtcgtgt tctggaacgc cagtgaaccc tggaaccagt ctgtataagc aaactcatcg
    57061 aaggagaatt cgtgattccc catgtttatt tcaacaatac ctctaccttg aagatcgtta
    57121 tcaaataccc aatctttatt ggcattgatt tgcagtttgc catcaacatc caggtccgca
    57181 cggccgatgt tcttgtcttc agaaataacc gcgacagcac cagaagcgac atcaatatat
    57241 ccgctgaatg catagttatt gccggtcagt gccactgtcc cactgtcgat acttattgta
    57301 ccattgccgg aaatcttatt ggcaaaggtc atgctatcgt cagccttcag gtgtaatttc
    57361 ccgcggttgt tgatctctgc tgtacctata tttttggtga tccctaaagc gccattttgc
    57421 tcaattttga ctttaccact aaaggcagag ttatcaccgg ttaagtaagt cgtgccattg
    57481 agcgcattga tatagcccag gccctcgctg ccactgacaa tatccgcgga aatagtcgcg
    57541 tttttatccg tgtggtttaa ggtgattttc ttatcggatg tctttgccca cacaaactca
    57601 attttttcgg catcaataat ccctgcagcc ttcgcagcac ttccttcctg tgcacctaac
    57661 gctaactcag agttggtgct taaactaatt ttcggtgcgg atattttacc tccatctgat
    57721 acgactaatg ttgtatcaga acgttggtcg taataaccca ggctaatatg ttcacgagca
    57781 ttaacttcac caccgttatt aaccgacagg aactgattac cgccatatcc gccgtaaacg
    57841 cccataaaga aggtccctgc atttacctga ctaccttcgc cgcttacatt cccctgcgta
    57901 taagtcagac gactggtgcc cttaacatga attttgccgc cattggagac attaaaatag
    57961 ccttgatcat gtctcatgcc tgagtttgac tcatacaaat catcagcata taactcgctg
    58021 ccagcattgg agacattcag gaaccccgta cctttcagca aaaagttaag cgggccattg
    58081 attgttacct ttcctccggt aatatccaac ccaccaccag aagcgagagt cagacgcgga
    58141 cgggaagtat ctgtcgtatt gattaccaga ttggcattgt cagtcaccgt aatacggtaa
    58201 tcgatgctct ggttctcttt gccaaaatca atagttttat cctggtttac gatgacatca
    58261 tcggcgaata ccagagatga atataaactt atcgcgctaa ttaaaacagc ccgggacttt
    58321 tttcctttta ctctgctccc cagctcagag cagacgctcc aacagttacg ggacctgttc
    58381 cactttagcc gatatatttt attcatcaca atctccagca atacatccaa ataaaaccaa
    58441 gattgcataa acagcaacgc attaaatgcc attctggtta ctgttcatcg catataaaaa
    58501 ttcaaaaatt ttgcatagca ttggcaacca catatctgag tatgtagttc agagggtaat
    58561 aaaatagcaa gttattactt aaaacttatt ttaagttaac actcttaact ttatcattac
    58621 attcctccca gtctctaata cctgcgcatt atatgtatcc aggaactcaa atcaactcaa
    58681 aatataaaca aaagcaagag gaatacattt aatatacagc cgataaaagc ggtaactctt
    58741 attaggtatc aaaatataag aatcgtctta ttaataaata tataacacta taggactgaa
    58801 tccattcagc taatacaaaa taaaatcgtt aattaataaa atataaaatc atgaagtgat
    58861 acaagaggaa ttgcaggata tgacatgaca atcatatata atatatgcat aattttttac
    58921 agcgagtcaa tgattcccgc gccatatgca gacatggcgc ggttttatgc aaaaacaatc
    58981 acgcgtccgg gaacatcacg ctatggcccg gcgcttcacg atggagatga ataaagttaa
    59041 ggtgccgctc gtactggtca agaatatcgg tgatcacctg ctctttgctg tagtccatca
    59101 ggtcgttgcc ctggctgcct tctaacagga aggtttccag ccggtagtag gtcgatttac
    59161 cgctacgtgc gcggtaggta aagcccggca ccgaatattg ctgcggccaa atctgataga
    59221 caaagttttg ttcttcgccc atatgcacca acaaatccag atgccccaac tgttgtccct
    59281 cttccggcgg caggcttttt agctccacgt atgcgccgcg caaccgcagc tcctgcgcca
    59341 cttcttccat tgccgggtaa cagaccgtct ccatcatctg tttagtgtaa cgcgtgcccg
    59401 gataattcat caggcgcgag agacgttttt tccagctcag gcgatcctga agccccagcg
    59461 gtcgcggtgc ggtatcgcgg ttggcacttt cacggcggta atcttctacc ttcagagatt
    59521 tatacaaccc cgccatcacg aagaagatca caaagctgaa cggcagcccc ataatcaccg
    59581 tggtgttttg cagcgcggat atcccgttgg tcatcagcat gccgagcgtc agcaggccaa
    59641 tcgccaccga ccagaagacg cgcagccagc cgggggcgtc gctgttgata tctttaagct
    59701 gcgaggtgaa attccccagc accagcgcac cggagtccgc cgaggtcaca taaaacagca
    59761 ggccagtaat ggtggcgacg gaggcgctaa aggtaaacgc cggatactgc gccagcaggc
    59821 tgtagaagcc gcgctccgga tggaccatcg cttcctcggc aaatgccgcg ccgccgtgga
    59881 tgatttcata cagcgcgcta ttgccgaaca ccgagagcca taacagcgtg aaggtaaacg
    59941 gaataatcaa cgtgcccagc acgaactggc gaatggtacg cccacgcgag atacgcgcca
    60001 ggaacaagcc gacaaacggc gaccatgcca cccaccatgc ccagaagaag agcgtccagt
    60061 tattcatcca ctcaactgga cggtcgaagg caaaactgtt gagcgtcatg cccataaagc
    60121 gattcacata gtcgccaaca ttcagcacca gcgcattaag caggaacgag gtgtcgccca
    60181 taaacaatac gaacaggatc aatcccagcg ccagcgcaac gttaagttcc gataacacac
    60241 gaatcccctt atcgacaccg gaggtgacag agatcgtggc gattatcacc gacaaggcga
    60301 tcagtgccgc ttttgccgcc atcgaatcgg gaatatcaaa cagtacgctc aagccatagt
    60361 taagctgcac cacaccgata ccgagcgtag tggcaatacc gaagatggtg ccgatcaccg
    60421 ctgcaatatc cactgagtgc cctatcggcc cgttaatccg tttaccgaag atcgggtaca
    60481 gcgccgagcg gatggtgagc ggcaaattat aacgatagct aaagtatccg agcgccatgc
    60541 ccatcagcgc atacatcgac cagccggtta agccgtagtg aaacagcgtc cagaccatcg
    60601 cctgacgcgc ggcctcaatc gtctgtcccg cgccttccgg cggctgcata tactgcgtta
    60661 ccggttcggc tacggagaag aacatcaggt cgataccgat cccggcagca aacagcatcg
    60721 ccgcccaact cagcaggctg aattccggtt tggattgttc tggcccgagc ttcaccgaac
    60781 caaaacgcga acaagcgata cagaccacaa agacaatata gagcgttgcc gccagtagat
    60841 agtaccaacc gaaggtttta gaaacccagt ccagcgtgcg gccaatccac agggccgaga
    60901 agtcgcgaaa caggatcgtt gtcagggaaa acaacaaaat cagtccggcg gaggtgtaaa
    60961 acaccaccgg attgattttg tccttttccc tgctgtgtga aaggtctgtc atccagtatc
    61021 cccactgtta ttgttaccat ttaaaatcaa attcgtaaca attaagacac attttatatt
    61081 gaacgtccaa tcaataaccg ctttaataga taaacaacgc tgatgaatgg agtggcgaaa
    61141 atgcccaaat tggggatgca gtcgatccgg cgcagacaac tgatcgacgc cacactggaa
    61201 gcaataaatg aagtgggcat gcacgatgca acgatcgcgc agatcgcccg ccgtgcaggc
    61261 gtttctacgg ggatcatcag ccactatttc agggacaaaa atggtctgct ggaagcaacc
    61321 atgcgcgata tcaccagtca gctgcgtgac gcggttttga atcgattaca tgcacttccg
    61381 cagggcagtg cagagcagcg attacaggcg attgttggcg gaaacttcga tgaaacgcag
    61441 gtgagcagtg cggcgatgaa agcctggctg gcgttctggg ccagcagtat gcatcagccg
    61501 atgctctatc gtttacagca ggtcagtagc cgccgcttat tgtcgaatct ggtgagcgaa
    61561 tttcgccgcg agttaccgcg ccaacaggca caggaagcgg gctacggcct tgccgcgctg
    61621 attgacggat tatggctgcg cgcggcactg agcggcaaac cgctggataa acctctcgca
    61681 cactcgctga cccgtcactt tattactcag catttaccca ccgattaacc gaggagacgt
    61741 gatgtcccga atggcagaac agcagcttta tatacatggt ggttatacct ccgccaccag
    61801 cggtcgcacc ttcgagacca ttaacccggc caacggtaac gtgctggcga ccgtgcaggc
    61861 cgccgggcgt gaggatgtcg atcgcgccgt gaagagtgcc caacaggggc aaaaaatctg
    61921 ggcggcgatg accgctatgg agcgctcgcg tattctgcgt cgggccgttg atattctgcg
    61981 tgaacgcaat gacgaactcg caaaactgga aaccctcgat accggaaaag catattcgga
    62041 aacctcaacc gtcgatatcg ttaccggtgc ggacgtgctg gagtactacg ccgggctgat
    62101 cccggcgctg gaaggcagcc agatcccgtt gcgtgagacg tcatttgttt atacccgccg
    62161 cgaaccgctg ggtgtggtgg cggggattgg cgcatggaac tacccgatcc agattgccct
    62221 gtggaaatcc gccccggcgc tggcggcagg caacgcaatg attttcaaac cgagcgaagt
    62281 caccccgctt accgcgttaa agctggctga aatttacagc gaagcgggcc tgccggacgg
    62341 cgtatttaac gtgttgccgg gggggggcgc ggagaccggg caatatctga ccgagcatcc
    62401 gggcattgcc aaagtgtcat ttaccggcgg tgtcgccagc ggcaaaaaag tgatggctaa
    62461 ctcggcggcc tcttccctga aagaagtgac catggaactg ggcggtaaat caccgctgat
    62521 cgttttcgac gatgcggatc tcgatctcgc cgccgatatc gccatgatgg cgaacttctt
    62581 cagctccggt caggtgtgta ccaacggcac ccgcgtcttc gttccggcga aatgcaaagc
    62641 cgcatttgag cagaaaattc tggcgcgcgt tgagcgcatt cgcgcgggcg acgttttcga
    62701 tccgcaaact aacttcagcc cgctggtcag cttcccgcat cgcgataacg tgctgcgcta
    62761 tatcgccaaa ggcaaagagg aaggcgcgcg cgtactgtgc ggcggcgatg tactgaaagg
    62821 cgatggcttc gataacggcg catgggttgc accgaccgtg ttcaccgatt gccgcgacga
    62881 aatgaccatc gtgcgcgaag agatcttcgg tccggtgatg tcgcttctca cttatgaatc
    62941 tgaggacgaa gtgattcgcc gcgccaatga taccgactac ggcctggcgg cgggcatcgt
    63001 gacggcggac ctgaaccgcg cgcatcgcgt cattcatcag ctggaagcgg gtatttgctg
    63061 gatcaacacc tggggcgaat ccccggcaga gatgcccgtt ggcggctaca aacactccgg
    63121 cattggtcgc gagaacggcg tgatgacgct ccagagttac acccaggtga agtccatcca
    63181 ggttgagatg gctaaattcc agtccatatt ctaaccagga ggtttatttg caatttgact
    63241 acatcattat tggtgccggc tcagccggca acgttctcgc tacccgtctg actgaagatc
    63301 cgaatacctc cgtgctgctg cttgaagcgg gcggcccgga ctatcgcttt gacttccgca
    63361 cccagatgcc cgcagcgctg gcgttcccac tacagggcaa acgctacaac tgggcctatg
    63421 aaacagagcc tgaaccgttt atgaataacc gccgcatgga gtgcggacgc ggtaaaggcc
    63481 tgggtggatc gtcgctgatc aacggcatgt gctacatccg tggcaatgcg ctggatctcg
    63541 ataactgggc gcaagaaccc ggtctggaga actggagtta tctcgactgc ctgccctact
    63601 accgcaaggc cgagactcgc gatgtgggcg agaacgacta ccacggtggc gatggcccgg
    63661 tgagcgtcac tacctccaaa cccggcgtca atccgctgtt tgaagcgatg attgaagcgg
    63721 gcgtgcaggc gggctacccg cgcacggacg atctcaacgg ttatcagcag gaaggttttg
    63781 gtccgatgga tcgcaccgtc acgccgcagg gccgtcgcgc cagtaccgcg cgcggttatc
    63841 tcgatcaggc caaatcgcgc cctaacctga ccattcgtat tcacgctatg accgatcaca
    63901 tcatttttga cggcaaacgc gcggtgggcg tcgaatggct ggaaggcgac agcaccatcc
    63961 caacccgcgc aacggccaac aaagaagtgc tgttatgtgc aggcgcgatt gcctcaccgc
    64021 agatcctgca acgctccggc gtcggcaacg ctgaactgct ggcggagttt gatattccgc
    64081 tggtgcatga attacccggc gtcggcgaaa atcttcagga tcatctggag atgtatctgc
    64141 aatatgagtg caaagaaccg gtttccctct accctgccct gcagtggtgg aaccagccga
    64201 aaatcggtgc ggagtggctg tttggcggca ctggcgttgg tgccagcaac cactttgaag
    64261 caggtggatt tattcgcagc cgtgaggaat ttgcgtggcc gaatattcag taccatttcc
    64321 tgccagtagc gattaactat aacggctcga atgcagtgaa agagcacggt ttccagtgcc
    64381 acgtcggctc aatgcgctcg ccaagccgtg ggcatgtgcg gattaaatcc cgcgacccgc
    64441 accagcatcc ggcgattctg tttaactaca tgtcgcacga gcaggactgg caggagttcc
    64501 gcgacgcaat tcgcatcacc cgcgagatca tgcatcaacc cgcgctggat cagtatcgtg
    64561 gccgcgaaat cagccccggc acggaatgtc agacggatga acagctcgat gagttcgtgc
    64621 gtaaccacgc cgaaaccgcc ttccatccgt gcggtacctg caaaatgggt tacgacgaga
    64681 tgtccgtggt tgacggcgaa ggccgcgtac acgggttaga aggcctgcgt gtggtggatg
    64741 cgtcgattat gccgcagatt atcaccggga atttgaacgc cacgacaatt atgattggcg
    64801 agaaaatagc ggatatgatt cgtggacagg aagcgctgcc gaggagcacg gcgggatatt
    64861 ttgtggcaaa tgggatgccg gtgagagcga aaaaatgagt cgtgatgtga actaacgcag
    64921 gaaccgatca gattgagagt taccgttcca gagaggggga ccgaatcctt atataaacac
    64981 cgaggtaact ctcatgcttc atatccagta tccatgatgc tggctttttg ctgatattta
    65041 acccgaacat tcctgataca gactattaat acaaccttat ctgcgacaga ttttattttc
    65101 tggatatatt atgcgtgaac aaatcaaaca ggatatcgat ctgattgaga ttttatttta
    65161 tctgaagaaa aaggttcgtg ttatc
//
LOCUS       DAZFWL020000011        64938 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFWL020000011 DAZFWL020000000
VERSION     DAZFWL020000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 64938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.011"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(154..230)
                     /locus_tag="ACHKNP_RS03885"
                     /old_locus_tag="pgaptmp_000777"
     tRNA            complement(154..230)
                     /locus_tag="ACHKNP_RS03885"
                     /old_locus_tag="pgaptmp_000777"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(194..196),aa:Arg,seq:acg)
     gene            complement(294..370)
                     /locus_tag="ACHKNP_RS03890"
                     /old_locus_tag="pgaptmp_000778"
     tRNA            complement(294..370)
                     /locus_tag="ACHKNP_RS03890"
                     /old_locus_tag="pgaptmp_000778"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(334..336),aa:Arg,seq:acg)
     gene            complement(568..644)
                     /locus_tag="ACHKNP_RS03895"
                     /old_locus_tag="pgaptmp_000779"
     tRNA            complement(568..644)
                     /locus_tag="ACHKNP_RS03895"
                     /old_locus_tag="pgaptmp_000779"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(608..610),aa:Arg,seq:acg)
     gene            complement(648..740)
                     /locus_tag="ACHKNP_RS03900"
                     /old_locus_tag="pgaptmp_000780"
     tRNA            complement(648..740)
                     /locus_tag="ACHKNP_RS03900"
                     /old_locus_tag="pgaptmp_000780"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(704..706),aa:Ser,seq:gct)
     gene            complement(1056..1241)
                     /gene="csrA"
                     /locus_tag="ACHKNP_RS03905"
                     /old_locus_tag="pgaptmp_000781"
     CDS             complement(1056..1241)
                     /gene="csrA"
                     /locus_tag="ACHKNP_RS03905"
                     /old_locus_tag="pgaptmp_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HGU4703003.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1476..4106)
                     /gene="alaS"
                     /locus_tag="ACHKNP_RS03910"
                     /old_locus_tag="pgaptmp_000782"
     CDS             complement(1476..4106)
                     /gene="alaS"
                     /locus_tag="ACHKNP_RS03910"
                     /old_locus_tag="pgaptmp_000782"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGU4703004.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(4234..4734)
                     /gene="recX"
                     /locus_tag="ACHKNP_RS03915"
                     /old_locus_tag="pgaptmp_000783"
     CDS             complement(4234..4734)
                     /gene="recX"
                     /locus_tag="ACHKNP_RS03915"
                     /old_locus_tag="pgaptmp_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGU4703005.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4977..6038)
                     /gene="recA"
                     /locus_tag="ACHKNP_RS03920"
                     /old_locus_tag="pgaptmp_000784"
     CDS             complement(4977..6038)
                     /gene="recA"
                     /locus_tag="ACHKNP_RS03920"
                     /old_locus_tag="pgaptmp_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGU4703006.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(6118..6615)
                     /gene="pncC"
                     /locus_tag="ACHKNP_RS03925"
                     /old_locus_tag="pgaptmp_000785"
     CDS             complement(6118..6615)
                     /gene="pncC"
                     /locus_tag="ACHKNP_RS03925"
                     /old_locus_tag="pgaptmp_000785"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HGU4703007.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6760..7845)
                     /gene="mltB"
                     /locus_tag="ACHKNP_RS03930"
                     /old_locus_tag="pgaptmp_000786"
     CDS             complement(6760..7845)
                     /gene="mltB"
                     /locus_tag="ACHKNP_RS03930"
                     /old_locus_tag="pgaptmp_000786"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HGU4703008.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYVQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            8101..8664
                     /gene="srlA"
                     /locus_tag="ACHKNP_RS03935"
                     /old_locus_tag="pgaptmp_000787"
                     /gene_synonym="gutA"
     CDS             8101..8664
                     /gene="srlA"
                     /locus_tag="ACHKNP_RS03935"
                     /old_locus_tag="pgaptmp_000787"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HGU4703009.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8661..9620
                     /gene="srlE"
                     /locus_tag="ACHKNP_RS03940"
                     /old_locus_tag="pgaptmp_000788"
                     /gene_synonym="gutE"
     CDS             8661..9620
                     /gene="srlE"
                     /locus_tag="ACHKNP_RS03940"
                     /old_locus_tag="pgaptmp_000788"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HGU4703010.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFREGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9631..10002
                     /gene="srlB"
                     /locus_tag="ACHKNP_RS03945"
                     /old_locus_tag="pgaptmp_000789"
                     /gene_synonym="gutB"
     CDS             9631..10002
                     /gene="srlB"
                     /locus_tag="ACHKNP_RS03945"
                     /old_locus_tag="pgaptmp_000789"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HGU4703011.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            10006..10785
                     /gene="srlD"
                     /locus_tag="ACHKNP_RS03950"
                     /old_locus_tag="pgaptmp_000790"
     CDS             10006..10785
                     /gene="srlD"
                     /locus_tag="ACHKNP_RS03950"
                     /old_locus_tag="pgaptmp_000790"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HGU4703012.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10890..11249
                     /gene="gutM"
                     /locus_tag="ACHKNP_RS03955"
                     /old_locus_tag="pgaptmp_000791"
     CDS             10890..11249
                     /gene="gutM"
                     /locus_tag="ACHKNP_RS03955"
                     /old_locus_tag="pgaptmp_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HGU4703013.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11316..12089
                     /gene="srlR"
                     /locus_tag="ACHKNP_RS03960"
                     /old_locus_tag="pgaptmp_000792"
                     /gene_synonym="gutR"
     CDS             11316..12089
                     /gene="srlR"
                     /locus_tag="ACHKNP_RS03960"
                     /old_locus_tag="pgaptmp_000792"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HGU4703014.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            12082..13047
                     /gene="gutQ"
                     /locus_tag="ACHKNP_RS03965"
                     /old_locus_tag="pgaptmp_000793"
     CDS             12082..13047
                     /gene="gutQ"
                     /locus_tag="ACHKNP_RS03965"
                     /old_locus_tag="pgaptmp_000793"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HGU4703015.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(13044..14558)
                     /gene="norR"
                     /locus_tag="ACHKNP_RS03970"
                     /old_locus_tag="pgaptmp_000794"
     CDS             complement(13044..14558)
                     /gene="norR"
                     /locus_tag="ACHKNP_RS03970"
                     /old_locus_tag="pgaptmp_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HGU4703016.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14745..16184
                     /gene="norV"
                     /locus_tag="ACHKNP_RS03975"
                     /old_locus_tag="pgaptmp_000795"
     CDS             14745..16184
                     /gene="norV"
                     /locus_tag="ACHKNP_RS03975"
                     /old_locus_tag="pgaptmp_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HGU4703017.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            16181..17314
                     /gene="norW"
                     /locus_tag="ACHKNP_RS03980"
                     /old_locus_tag="pgaptmp_000796"
     CDS             16181..17314
                     /gene="norW"
                     /locus_tag="ACHKNP_RS03980"
                     /old_locus_tag="pgaptmp_000796"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HGU4703018.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17540..19792)
                     /gene="hypF"
                     /locus_tag="ACHKNP_RS03985"
                     /old_locus_tag="pgaptmp_000797"
     CDS             complement(17540..19792)
                     /gene="hypF"
                     /locus_tag="ACHKNP_RS03985"
                     /old_locus_tag="pgaptmp_000797"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HGU4703019.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19945..20472)
                     /gene="hydN"
                     /locus_tag="ACHKNP_RS03990"
                     /old_locus_tag="pgaptmp_000798"
     CDS             complement(19945..20472)
                     /gene="hydN"
                     /locus_tag="ACHKNP_RS03990"
                     /old_locus_tag="pgaptmp_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HGU4703020.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGVGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20621..21631)
                     /gene="ascG"
                     /locus_tag="ACHKNP_RS03995"
                     /old_locus_tag="pgaptmp_000799"
     CDS             complement(20621..21631)
                     /gene="ascG"
                     /locus_tag="ACHKNP_RS03995"
                     /old_locus_tag="pgaptmp_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HGU4703021.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGRWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLICRDSLIAL
                     SR"
     gene            21891..23348
                     /gene="ascF"
                     /locus_tag="ACHKNP_RS04000"
                     /old_locus_tag="pgaptmp_000800"
     CDS             21891..23348
                     /gene="ascF"
                     /locus_tag="ACHKNP_RS04000"
                     /old_locus_tag="pgaptmp_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HGU4703022.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            23357..24781
                     /gene="ascB"
                     /locus_tag="ACHKNP_RS04005"
                     /old_locus_tag="pgaptmp_000801"
     CDS             23357..24781
                     /gene="ascB"
                     /locus_tag="ACHKNP_RS04005"
                     /old_locus_tag="pgaptmp_000801"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HGU4703023.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSITWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24895..25314)
                     /locus_tag="ACHKNP_RS04010"
                     /old_locus_tag="pgaptmp_000802"
     CDS             complement(24895..25314)
                     /locus_tag="ACHKNP_RS04010"
                     /old_locus_tag="pgaptmp_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4703024.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(25311..25622)
                     /locus_tag="ACHKNP_RS04015"
                     /old_locus_tag="pgaptmp_000803"
     CDS             complement(25311..25622)
                     /locus_tag="ACHKNP_RS04015"
                     /old_locus_tag="pgaptmp_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HGU4703025.1"
                     /translation="MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25796..26539)
                     /locus_tag="ACHKNP_RS04020"
                     /old_locus_tag="pgaptmp_000804"
     CDS             complement(25796..26539)
                     /locus_tag="ACHKNP_RS04020"
                     /old_locus_tag="pgaptmp_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703026.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(26582..27052)
                     /gene="hycI"
                     /locus_tag="ACHKNP_RS04025"
                     /old_locus_tag="pgaptmp_000805"
     CDS             complement(26582..27052)
                     /gene="hycI"
                     /locus_tag="ACHKNP_RS04025"
                     /old_locus_tag="pgaptmp_000805"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HGU4703027.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(27045..27455)
                     /gene="hycH"
                     /locus_tag="ACHKNP_RS04030"
                     /old_locus_tag="pgaptmp_000806"
     CDS             complement(27045..27455)
                     /gene="hycH"
                     /locus_tag="ACHKNP_RS04030"
                     /old_locus_tag="pgaptmp_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HGU4703028.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(27452..28219)
                     /gene="hycG"
                     /locus_tag="ACHKNP_RS04035"
                     /old_locus_tag="pgaptmp_000807"
     CDS             complement(27452..28219)
                     /gene="hycG"
                     /locus_tag="ACHKNP_RS04035"
                     /old_locus_tag="pgaptmp_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HGU4703029.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(28219..28761)
                     /gene="hycF"
                     /locus_tag="ACHKNP_RS04040"
                     /old_locus_tag="pgaptmp_000808"
     CDS             complement(28219..28761)
                     /gene="hycF"
                     /locus_tag="ACHKNP_RS04040"
                     /old_locus_tag="pgaptmp_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HGU4703030.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28771..30480)
                     /gene="hycE"
                     /locus_tag="ACHKNP_RS04045"
                     /old_locus_tag="pgaptmp_000809"
     CDS             complement(28771..30480)
                     /gene="hycE"
                     /locus_tag="ACHKNP_RS04045"
                     /old_locus_tag="pgaptmp_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HGU4703031.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(30498..31421)
                     /gene="hycD"
                     /locus_tag="ACHKNP_RS04050"
                     /old_locus_tag="pgaptmp_000810"
     CDS             complement(30498..31421)
                     /gene="hycD"
                     /locus_tag="ACHKNP_RS04050"
                     /old_locus_tag="pgaptmp_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HGU4703032.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(31424..33250)
                     /gene="hycC"
                     /locus_tag="ACHKNP_RS04055"
                     /old_locus_tag="pgaptmp_000811"
     CDS             complement(31424..33250)
                     /gene="hycC"
                     /locus_tag="ACHKNP_RS04055"
                     /old_locus_tag="pgaptmp_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HGU4703033.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMRVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(33247..33858)
                     /gene="hycB"
                     /locus_tag="ACHKNP_RS04060"
                     /old_locus_tag="pgaptmp_000812"
     CDS             complement(33247..33858)
                     /gene="hycB"
                     /locus_tag="ACHKNP_RS04060"
                     /old_locus_tag="pgaptmp_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HGU4703034.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33983..34444)
                     /gene="hycA"
                     /locus_tag="ACHKNP_RS04065"
                     /old_locus_tag="pgaptmp_000813"
     CDS             complement(33983..34444)
                     /gene="hycA"
                     /locus_tag="ACHKNP_RS04065"
                     /old_locus_tag="pgaptmp_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HGU4703035.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            34656..35006
                     /gene="hypA"
                     /locus_tag="ACHKNP_RS04070"
                     /old_locus_tag="pgaptmp_000814"
     CDS             34656..35006
                     /gene="hypA"
                     /locus_tag="ACHKNP_RS04070"
                     /old_locus_tag="pgaptmp_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU4703036.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            35010..35882
                     /gene="hypB"
                     /locus_tag="ACHKNP_RS04075"
                     /old_locus_tag="pgaptmp_000815"
     CDS             35010..35882
                     /gene="hypB"
                     /locus_tag="ACHKNP_RS04075"
                     /old_locus_tag="pgaptmp_000815"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HGU4703037.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCLACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35873..36145
                     /gene="hypC"
                     /locus_tag="ACHKNP_RS04080"
                     /old_locus_tag="pgaptmp_000816"
     CDS             35873..36145
                     /gene="hypC"
                     /locus_tag="ACHKNP_RS04080"
                     /old_locus_tag="pgaptmp_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HGU4703038.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            36145..37266
                     /gene="hypD"
                     /locus_tag="ACHKNP_RS04085"
                     /old_locus_tag="pgaptmp_000817"
     CDS             36145..37266
                     /gene="hypD"
                     /locus_tag="ACHKNP_RS04085"
                     /old_locus_tag="pgaptmp_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HGU4703039.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            37263..38273
                     /gene="hypE"
                     /locus_tag="ACHKNP_RS04090"
                     /old_locus_tag="pgaptmp_000818"
     CDS             37263..38273
                     /gene="hypE"
                     /locus_tag="ACHKNP_RS04090"
                     /old_locus_tag="pgaptmp_000818"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HGU4703040.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            38347..40425
                     /gene="flhA"
                     /locus_tag="ACHKNP_RS04095"
                     /old_locus_tag="pgaptmp_000819"
     CDS             38347..40425
                     /gene="flhA"
                     /locus_tag="ACHKNP_RS04095"
                     /old_locus_tag="pgaptmp_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HGU4703041.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(40462..40815)
                     /gene="ygbA"
                     /locus_tag="ACHKNP_RS04100"
                     /old_locus_tag="pgaptmp_000820"
     CDS             complement(40462..40815)
                     /gene="ygbA"
                     /locus_tag="ACHKNP_RS04100"
                     /old_locus_tag="pgaptmp_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HGU4703042.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40892..41026)
                     /locus_tag="ACHKNP_RS04105"
                     /old_locus_tag="pgaptmp_000821"
     CDS             complement(40892..41026)
                     /locus_tag="ACHKNP_RS04105"
                     /old_locus_tag="pgaptmp_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703043.1"
                     /translation="MIPELIISGEEANCEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            41102..43663
                     /gene="mutS"
                     /locus_tag="ACHKNP_RS04110"
                     /old_locus_tag="pgaptmp_000822"
     CDS             41102..43663
                     /gene="mutS"
                     /locus_tag="ACHKNP_RS04110"
                     /old_locus_tag="pgaptmp_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGU4703044.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43769..44425
                     /gene="pphB"
                     /locus_tag="ACHKNP_RS04115"
                     /old_locus_tag="pgaptmp_000823"
     CDS             43769..44425
                     /gene="pphB"
                     /locus_tag="ACHKNP_RS04115"
                     /old_locus_tag="pgaptmp_000823"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU4703045.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(44476..45243)
                     /gene="ygbI"
                     /locus_tag="ACHKNP_RS04120"
                     /old_locus_tag="pgaptmp_000824"
     CDS             complement(44476..45243)
                     /gene="ygbI"
                     /locus_tag="ACHKNP_RS04120"
                     /old_locus_tag="pgaptmp_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HGU4703046.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            45439..46347
                     /gene="ltnD"
                     /locus_tag="ACHKNP_RS04125"
                     /old_locus_tag="pgaptmp_000825"
     CDS             45439..46347
                     /gene="ltnD"
                     /locus_tag="ACHKNP_RS04125"
                     /old_locus_tag="pgaptmp_000825"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HGU4703047.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            46344..47606
                     /gene="otnK"
                     /locus_tag="ACHKNP_RS04130"
                     /old_locus_tag="pgaptmp_000826"
     CDS             46344..47606
                     /gene="otnK"
                     /locus_tag="ACHKNP_RS04130"
                     /old_locus_tag="pgaptmp_000826"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HGU4703048.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            47603..48241
                     /gene="ygbL"
                     /locus_tag="ACHKNP_RS04135"
                     /old_locus_tag="pgaptmp_000827"
     CDS             47603..48241
                     /gene="ygbL"
                     /locus_tag="ACHKNP_RS04135"
                     /old_locus_tag="pgaptmp_000827"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HGU4703049.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            48246..49022
                     /gene="ygbM"
                     /locus_tag="ACHKNP_RS04140"
                     /old_locus_tag="pgaptmp_000828"
     CDS             48246..49022
                     /gene="ygbM"
                     /locus_tag="ACHKNP_RS04140"
                     /old_locus_tag="pgaptmp_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HGU4703050.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            49111..50475
                     /gene="ygbN"
                     /locus_tag="ACHKNP_RS04145"
                     /old_locus_tag="pgaptmp_000829"
     CDS             49111..50475
                     /gene="ygbN"
                     /locus_tag="ACHKNP_RS04145"
                     /old_locus_tag="pgaptmp_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HGU4703051.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(50514..50750)
                     /locus_tag="ACHKNP_RS04150"
                     /old_locus_tag="pgaptmp_000830"
     CDS             complement(50514..50750)
                     /locus_tag="ACHKNP_RS04150"
                     /old_locus_tag="pgaptmp_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HGU4703052.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTESLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(50761..52188)
                     /locus_tag="ACHKNP_RS04155"
                     /old_locus_tag="pgaptmp_000831"
     CDS             complement(50761..52188)
                     /locus_tag="ACHKNP_RS04155"
                     /old_locus_tag="pgaptmp_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HGU4703053.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(52188..52781)
                     /locus_tag="ACHKNP_RS04160"
                     /old_locus_tag="pgaptmp_000832"
     CDS             complement(52188..52781)
                     /locus_tag="ACHKNP_RS04160"
                     /old_locus_tag="pgaptmp_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HGU4703054.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            52928..53335
                     /locus_tag="ACHKNP_RS04165"
                     /old_locus_tag="pgaptmp_000833"
     CDS             52928..53335
                     /locus_tag="ACHKNP_RS04165"
                     /old_locus_tag="pgaptmp_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGU4703055.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(53456..54448)
                     /gene="rpoS"
                     /locus_tag="ACHKNP_RS04170"
                     /old_locus_tag="pgaptmp_000834"
     CDS             complement(53456..54448)
                     /gene="rpoS"
                     /locus_tag="ACHKNP_RS04170"
                     /old_locus_tag="pgaptmp_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HGU4703056.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(54511..55605)
                     /gene="nlpD"
                     /locus_tag="ACHKNP_RS04175"
                     /old_locus_tag="pgaptmp_000835"
     CDS             complement(54511..55605)
                     /gene="nlpD"
                     /locus_tag="ACHKNP_RS04175"
                     /old_locus_tag="pgaptmp_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HGU4703057.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(55790..56416)
                     /gene="pcm"
                     /locus_tag="ACHKNP_RS04180"
                     /old_locus_tag="pgaptmp_000836"
     CDS             complement(55790..56416)
                     /gene="pcm"
                     /locus_tag="ACHKNP_RS04180"
                     /old_locus_tag="pgaptmp_000836"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HGU4703058.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(56410..57171)
                     /gene="surE"
                     /locus_tag="ACHKNP_RS04185"
                     /old_locus_tag="pgaptmp_000837"
     CDS             complement(56410..57171)
                     /gene="surE"
                     /locus_tag="ACHKNP_RS04185"
                     /old_locus_tag="pgaptmp_000837"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HGU4703059.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(57152..58201)
                     /gene="truD"
                     /locus_tag="ACHKNP_RS04190"
                     /old_locus_tag="pgaptmp_000838"
     CDS             complement(57152..58201)
                     /gene="truD"
                     /locus_tag="ACHKNP_RS04190"
                     /old_locus_tag="pgaptmp_000838"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HGU4703060.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLNDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(58198..58677)
                     /gene="ispF"
                     /locus_tag="ACHKNP_RS04195"
                     /old_locus_tag="pgaptmp_000839"
     CDS             complement(58198..58677)
                     /gene="ispF"
                     /locus_tag="ACHKNP_RS04195"
                     /old_locus_tag="pgaptmp_000839"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HGU4703061.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(58677..59387)
                     /gene="ispD"
                     /locus_tag="ACHKNP_RS04200"
                     /old_locus_tag="pgaptmp_000840"
     CDS             complement(58677..59387)
                     /gene="ispD"
                     /locus_tag="ACHKNP_RS04200"
                     /old_locus_tag="pgaptmp_000840"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGU4703062.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(59406..59717)
                     /gene="ftsB"
                     /locus_tag="ACHKNP_RS04205"
                     /old_locus_tag="pgaptmp_000841"
     CDS             complement(59406..59717)
                     /gene="ftsB"
                     /locus_tag="ACHKNP_RS04205"
                     /old_locus_tag="pgaptmp_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HGU4703063.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(59911..60234)
                     /gene="ygbE"
                     /locus_tag="ACHKNP_RS04210"
                     /old_locus_tag="pgaptmp_000842"
     CDS             complement(59911..60234)
                     /gene="ygbE"
                     /locus_tag="ACHKNP_RS04210"
                     /old_locus_tag="pgaptmp_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HGU4703064.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(60284..60889)
                     /gene="cysC"
                     /locus_tag="ACHKNP_RS04215"
                     /old_locus_tag="pgaptmp_000843"
     CDS             complement(60284..60889)
                     /gene="cysC"
                     /locus_tag="ACHKNP_RS04215"
                     /old_locus_tag="pgaptmp_000843"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HGU4703065.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(60889..62316)
                     /gene="cysN"
                     /locus_tag="ACHKNP_RS04220"
                     /old_locus_tag="pgaptmp_000844"
     CDS             complement(60889..62316)
                     /gene="cysN"
                     /locus_tag="ACHKNP_RS04220"
                     /old_locus_tag="pgaptmp_000844"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HGU4703066.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGS
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(62318..63226)
                     /gene="cysD"
                     /locus_tag="ACHKNP_RS04225"
                     /old_locus_tag="pgaptmp_000845"
     CDS             complement(62318..63226)
                     /gene="cysD"
                     /locus_tag="ACHKNP_RS04225"
                     /old_locus_tag="pgaptmp_000845"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HGU4703067.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            63478..64515
                     /gene="iap"
                     /locus_tag="ACHKNP_RS04230"
                     /old_locus_tag="pgaptmp_000846"
     CDS             63478..64515
                     /gene="iap"
                     /locus_tag="ACHKNP_RS04230"
                     /old_locus_tag="pgaptmp_000846"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HGU4703068.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   64597..64930
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=64658..64686
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
BASE COUNT        15257 a        17752 c        16828 g        15101 t
ORIGIN      
        1 gccatatcaa gtaagagatg gtgcatccgg gaggattact cgccttgcgg ctcgcccttc
       61 gggccgttgc taaagcaacg ttatcatccc tggtgcctgc ggttaattcc gctacctctt
      121 cgataccttc attgctgaag atttcggaga atatggtgca tccgggagga ttcgaacctc
      181 cgaccgctcg gttcgtagcc gagtactcta tccagctgag ctacggatgc atcgggaaaa
      241 tttattttac tgctgatatt gataccgcta ctaaagccat atcaagtaag agatggtgca
      301 tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta tccagctgag
      361 ctacggatgc atcgggaaac ttactttact gcagattttt tgataccgct actaaagccg
      421 tatcaagtaa gagatggtgc atccgggagg attactcgcc ttgcggctcg cccttcgggc
      481 cgttgctaaa gcaacgttat cctccctggt gcctgcggtt ataccgccac ctcttccata
      541 ccttcatcgc tgaaagtacg tagaatatgg tgcatccggg aggattcgaa cctccgaccg
      601 ctcggttcgt agccgagtac tctatccagc tgagctacgg atgcaaatgg cggtgaggcg
      661 gggattcgaa ccccggatgc agcttttgac cgcatactcc cttagcaggg gagcgccttc
      721 agcctctcgg ccacctcacc acacgcctct tacgagtgct tcgaagaact tgtttatcgc
      781 tcatcgtcgc tgcgtggcgc acatattact ttctgagact tataagtcaa acaatttttc
      841 ctacactttt atcgtttgca cacttcacgt tcaattagtc tgtaaaaacg tcaaaaagag
      901 tgttttatca acagaaaaat ggaggtctga cagatagtag taatgcaaaa aaatggagac
      961 ttaagttgaa tgaacgggag taaagcgaaa agactataga gtgaaggaga aattttgagg
     1021 gtgcgtctca ccgataaaga tgagacgcgg aaagattagt aactggactg ctgggatttt
     1081 tcagcctgga tacgctggta gatctcttca cggtgaacag aaacttcctt cggggcattt
     1141 acgccaatac gtacctggtt gccctttacc cctaaaactg tcacggtgac ctcatcccca
     1201 atcatgaggg tctcaccaac tcgacgagtc agaatcagca ttctttgctc cttgaaagat
     1261 taaaagagtc gggtctctct gtatcccggc attatccatc atataacgcc aaaaagtaag
     1321 caatgacaaa cacattacat gtaagcagtc atggcattac attctgttaa acctaagttt
     1381 agccgatata cacaacttca acctgacttt atcgttgtcg atagcgttaa tgcgaatgcc
     1441 gtgaagcgag tccacggcat tgcctgacgc ttatattatt gcaatttcgc gctgacccag
     1501 cctttcacac tggctaacgc tgcaggtaag gccgcagcat ccgtaccacc ggcttgcgcc
     1561 atgtcaggac gtccaccacc cttgccgccc acctgctgag cgaccatacc aatcagttcc
     1621 cctgctttca cacgatctgt gacgtcctta gatacgcctg caatcagaga aaccttacct
     1681 tcggctaccg ttgccagcac gataattgtc gaccccagct gattttttaa atcgtcaacc
     1741 atggtacgca acattttcgg ctcaacaccg ctaagctcgc taaccaacag cttaacacca
     1801 ttaacatcaa ttgccttact ggaaagattt gcgctctcct gtgcggcagc ttgttcttta
     1861 agctgttgta gttctttttc taactgacgc gtacgttcca gtactgagcg cactttatcc
     1921 gccagattat tgctatcgcc tttcagcaga tgcgcgactt cgcttaagcg atcgctgtct
     1981 gcatgaacgg tggtgatagc gccttctccg gttaccgctt cgatacgacg aacgcctgca
     2041 gcagtacccg attcagagat gatgcggaac agaccaatat caccagtgcg gctggcgtga
     2101 gtaccgccac acagctcggt ggagaaatcg cccatgctca gcacgcgtac acgctcatca
     2161 tacttctcgc cgaacagcgc catcgcacct ttcgctttcg ccgcttcgag atccatgatg
     2221 ttggtttcga tcggcaaatt gcgacgaatc tgtgcgttca ccaggtcttc gaccgcacga
     2281 atctcttccg gtttcatcgc ttcgttgtgt gagaagtcga agcgcagcac cttgtcgtta
     2341 accagtgaac ctttctgcga tacatgagtc cccagaacct ggcgcagcgc agcgtgcatc
     2401 aggtgcgttg cggagtgatt cagacgaata cgggcgcgac gagcctcatc aacatcagcc
     2461 tgcacagcgt cgcccacttt cagagaaccc gcagcaagtt taccgatgtg accaatcgcc
     2521 tggccgtatt tctgcgtatc ttccaccgcg aaggagaagt tagcgccttt cagttcgcct
     2581 ttatcaccaa cctgaccgcc ggattccgca tagaatggtg tttgatccag cacgaccaca
     2641 gcttcctggc ctgcattgat tgcatcaacc gctttaccat caacaaacag cgcggtcact
     2701 ttgccgttca gttccagatg gtcatagcct ttaaattcag atgcactatc aacacggatc
     2761 attgcgttgt aatcggcacc aaagccgctg gcttcgcgcg cgcgacgacg ctgctcttcc
     2821 attgctgctt caaaaccagc ttcgtcaact ttgatgttgc gctcacgaca aacatcagcc
     2881 gtcaggtcaa ccgggaagcc ataggtgtcg tacagacgga aagccgtttc accatccagc
     2941 gtatcaccag aaagttttgc cagctcttca tccagcaacg ccagaccgcg ctccagagta
     3001 cgggcaaact gctcttcttc agtcttcagc acctgctcaa cctgcgcctg ctggcgtttc
     3061 aggtcttcac ccgcagagcc cataacgtcg atcagcggac caaccagttt gtagaagaag
     3121 gtttctttcg cgccgagcat attgccgtga cgcactgcgc gacgaatgat acgacgcagt
     3181 acataaccac ggttttcatt ggacggcatt acgccatccg cgatcaggaa cgcacaagaa
     3241 cgaatgtggt cagcgattac gcgcagcgat ttattgctca gatcggtcgc gccagtgact
     3301 ttcgctaccg cctggatcaa cgtgcggaac aggtcgatgt catagttaga gttaacgtgt
     3361 tgcagcaccg cagcaatacg ctccagaccc ataccggtat ctacagacgg cttcggcagc
     3421 ggttccatcg tgccatctgc ctggcggttg aactgcatga agacgatgtt ccagatctca
     3481 atgtagcggt cgccgtcttc ttccggactt cccggaggac caccccaaat gtggtcgccg
     3541 tgatcgtaga agatttcggt gcacgggccg cacggaccag tgtcacccat ctgccagaag
     3601 ttgtcagatg cgtatggcgc acctttgtta tcgccgatgc gaataatacg ttcgcgcggg
     3661 atcccgactt ctttttccca gatttcgtag gcttcgtcgt cgctttcata gacggtaacc
     3721 cacagacgct ctttcggcag ggcaaaccat ttttcgctgg tcagcagttc ccatgcaaac
     3781 tgaatggcat cgtgtttgaa atagtcgccg aagctgaagt tgcccagcat ttcgaagaag
     3841 gtatggtgac gcgcggtgta accgacgttt tccaggtcgt tgtgtttacc acccgcacgc
     3901 acgcagcgtt gggaagtggt agcgcgggaa taattacgct tgtcgagccc aaggaacaca
     3961 tccttgaact ggttcatccc ggcgttggta aacaacaaag ttgggtcgtt atggggtacc
     4021 agggagctgc tggcaactac ctgatgtccc ttactatgga aaaagtcgag aaacgcctga
     4081 cggatctcag cggtgctctt gctcataatt atcctgaaat caagctaacg aaatatcgcc
     4141 accagctcca gcgtgtctta accgccgggc tggtaactga aaagtgggaa taagataagt
     4201 tttcttgact gggaagtaaa ataccgtatg cgttcagtcg gcaaaatttc gccaaatatc
     4261 ctggatatct tccatcagat agccacgata gagcagaaaa cgctggatct taactttttc
     4321 tgaaaagaca gttggcaaag gttcaccata ttttcgcgtc gcctgatcgc gcgccagtgc
     4381 gcaccagtcg atgtcacatt cacgcatcgc tttttctgtc gcttcgcggg aaataccttt
     4441 ctgattcagt tcctgacgaa tacgtgcagg tccataacct ttgcggctac ggctggcgat
     4501 aaagcgcgca acaaatcggc tgtcatcgag atagccatgt tcatggcacc aggcaataac
     4561 gcgctcgtaa tcttctgccg tagcatcaat ctcttctggg ccatttttgc ccataatcgg
     4621 tgccacgagt ttacgtcgca gttcttgctc actgtgatcg cgcaccgcca gaatgcgtac
     4681 cgcacgatcc aacaggcgag catatgccgg gcgacgggac gttgattctg tcatggcata
     4741 tccttacaac ttaaaaaagc aaaagggccg cagatgcgac ccaggttttg ctgataaaca
     4801 gatttaatag tcaatgagtg tgcagaatgt tcccgcaggc ccaattcgtt agaggatcga
     4861 ggtgagagta atttttcacg ccagtgctgg cctgttcccc tccccctaac cctctcccca
     4921 aaggggcgag gggaccgtcc gtgcacacat tatctattat catcaataac gaacgattaa
     4981 aaatcttcgt tagtttctgc tacgccttcg ctgtcatcta cagagaaatc cggcgttgag
     5041 ttcgggttgc tcagcagcaa ctcacgtact ttcttctcga tctctttcgc ggtttccggg
     5101 ttatctttca gccaggcagt tgcattcgct ttaccctgac cgatcttctc acctttgtag
     5161 ctgtaccacg cgcctgcttt ctcgatcagc ttctctttta cgcccaggtc aaccagttcg
     5221 ccgtagaagt tgataccttc gccgtagagg atctgaaatt cagcctgttt aaacggtgca
     5281 gcgattttgt tcttcaccac tttcacgcgg gtttcgctac ccaccacgtt ttcgccctct
     5341 ttcaccgcgc cgatacgacg gatgtcgaga cgaacagagg cgtagaattt cagcgcgtta
     5401 ccaccggtag tggtttccgg gttaccgaac atcacaccaa ttttcatacg gatctggttg
     5461 atgaagatca gcagcgtgtt ggactgcttc aggttacccg ccagcttacg catcgcctgg
     5521 ctcatcatac gtgccgcaag gcccatgtga gagtcgccga tttcgccttc gatttccgct
     5581 ttcggcgtca gtgccgccac ggagtcaacg acgataacgt ctactgcgcc agaacgcgcc
     5641 agggcgtcac agatttccag tgcctgctcg ccggtgtccg gctgggagca cagcaggtta
     5701 tcgatatcga cgcccagttt acgtgcgtag attgggtcca gcgcgtgttc agcatcgata
     5761 aacgcacagg ttttaccttc acgctgcgct gcggcgatca cctgcagcgt cagcgtggtt
     5821 ttaccggaag attccggtcc gtagatttcg acgatacggc ccatcggcag accacctgcc
     5881 ccaagcgcga tatccagtga aagcgaaccg gtagagatgg tttccacatc catggaacgg
     5941 tcttcaccca ggcgcatgat ggagccttta ccaaattgtt tctcaatctg gcccagtgct
     6001 gccgccaacg ctttctgttt gttttcgtcg atagccattt ttactcctgt catgccgggt
     6061 aataccggat agtcaatatg ttctgttgaa gcaattatac tgtatgctca tacagtatca
     6121 agtgttttgt agaaattgtt gccacaaggt ctgcaatgca tacgcagtag cctgacgacg
     6181 caccgcatca cggtcaccgc tgaagcattc ccgccgggta atgccttcac cgcgggcagt
     6241 ggcaaaagca aaccagacgg tgccgacagg cttctcttca ctgccgccat ccggcccggc
     6301 gataccacta atagacacgg cataatcagc acgagccgct ttcagtgcac ctatcgccat
     6361 ttccaccacg acgggttcac tcaccgcgcc atgctgcgcc agcgtctctt cgcgtacgcc
     6421 gatcatctgc gctttggctt cgttactgta ggtgacaaat ccgcgttcaa accaggcgga
     6481 gctaccggca atatcggtaa tcactttcgc tacccaacca ccggtacaag actcggcagt
     6541 tgttacggtt gcgccacggg ctttcagcgc ctgcccaacc tgttcactta actgcatcag
     6601 ttcactgtca gtcatcacaa cctctgtcag tcaaaaattt atgccggaca agatagcact
     6661 ttcgtcagga agatggtgtt ggcgttgcaa ttttacgagg aggattcaga aaaaagctga
     6721 ttagccagag ggaagctcac gcccccctct tgcaaatagt tactgcacgc gcgccagcgc
     6781 cacggcttgt cctaactgcc agaccgccat tgcgtaatgg gtgctgtggt tataacgggt
     6841 aatggtgtag aagttcggca gaccgtacca gtactggtaa ccggtaccga catccagacg
     6901 cagcaggctg gcttgttgat ggttgcccag cggctgctgt ggcgttaaac ctgccgtggc
     6961 aagctgcgaa atgctgtacc tggttttgaa gccatttggc aagcctggag cctgaccgtt
     7021 tgccattacc gcgacctgat cgcctttcac ccagccgtgc gctttgaaat agttcgccac
     7081 gctaccgatc gcatcaaccg gatcccacag gttgatatgc ccgtcgccgc tgaaatctac
     7141 cgcatattgt ttgtaagacg acggcataaa ctgtacgtag cccatcgccc cggcaaagga
     7201 acctttcaga ttgagcggat cgtcctgctc gtcgcgcgcc atcaacagga aggtttccag
     7261 ctcgccagag aaatactccg cgcggcgtgg gtagttaaat gacagcgttg ccagcgcatc
     7321 gaggatgcga gttttcccca tcacgcgccc ccagcgggtt tcaacgccga taatcccgac
     7381 gataatttcc ggcggtacgc catacacctg ccacgcgcga ttcaacgcat cttcatactg
     7441 attccagaaa accacaccgt tctgcacgtt gtccggcgta ataaattttt tgcgataacg
     7501 gagccatgcg ccgttcggac ctgatggcgg tttcaccgat gtggttggtg cctggttatc
     7561 catcagccgc agtaccgaat ccagacgctt cgcctgggag agaatttcct gcaactgctg
     7621 acgatcgaaa ccgtgtttgt tcaccatttt gtcgatgaac tgctgggcat tcgggttatt
     7681 agcgaaatcg ccgcccatct gcatcacatt gtgctgcggc tcaagcagga agccgccaga
     7741 cggcgttccg gtggtcgttt cagtctcagt aggttttggc ttgctgctac aggcggcaag
     7801 caacacaaaa aggggaagca atgttacata acgacgcttg aacatgaggg gtccatttaa
     7861 cagattcaac caggggcaag tatggtaaag catcacgccc cacacaagga agcggtagtc
     7921 actgcccgat acggacttta cataactcaa ctcattcccc tcgctatcct tttactcaaa
     7981 ctttcaaatt aaaatattta tctttcattt tgagatcaaa ataacacttt taaatctttc
     8041 aatctgatta gattaggttg ccgtttggta ataaaacaat aaatcctgaa ggagagaaca
     8101 atgatagaaa ccattactca tggtgcagag tggtttatcg ggctgttcca aaagggcgga
     8161 gaggtgttta ccgggatggt gaccggcatt cttccgctgt tgattagcct gctggttatc
     8221 atgaacgcac tgattaattt tatcggtcag catcgtattg aacgttttgc tcaacgttgc
     8281 gccggtaacc ctgtttcccg ttacctactg ttaccgtgca ttggcacgtt tgtcttttgc
     8341 aatccgatga ccttaagcct gggtcgcttt atgccggaaa agtacaaacc cagctactac
     8401 gcggcggcct cttatagctg ccactcaatg aatggcctct tcccccatat caaccctggc
     8461 gaactgtttg tttatcttgg cattgccagc ggtctgacaa cgctgaacct gccacttggc
     8521 ccactggcgg tgagttatct gctggttggt ctggtcacca atttcttccg cggctgggtg
     8581 accgatctga ccaccgccat ttttgagaaa aagatgggca ttcaacttga acaaaaagtt
     8641 cacctggcag gagcaacatc atgacgcata ttcggatcga aaaaggaacg ggtggctggg
     8701 gcggcccgct tgagctgaaa gccacgccgg gaaaaaaaat cgtctatatc accgccggta
     8761 cccggcctgc gattgttgac aaactggcac agcttactgg ctggcaggct attgacggat
     8821 ttagagaagg tgaacccgcg gaggcggaaa ttggtgtcgc ggtaatcgac tgtggcggca
     8881 cattacgctg cggcatctat ccgaaacgac gtattcccac cattaatatc cactcgacgg
     8941 gcaagtccgg tccgctggcg cagtacattg tggaagatat ttatgtctct ggcgtaaaag
     9001 aagaaaacat cactgtagtg ggtgatgcga caccacaacc ctcttccgtg ggccgtgact
     9061 atgacaccag taagaaaatc accgaacaaa gcgatggttt actggcgaag gtgggaatgg
     9121 gcatggggtc cgccgttgcg gtgctgtttc aatctggtcg tgacaccatc gacactgtat
     9181 taaaaaccat tctgccgttt atggcattcg tctcggcgct cattggcatc attatggctt
     9241 ctggccttgg tgactggatt gcccacggtc ttgctccgct ggcgagccat ccactgggtc
     9301 tggtcatgct ggcgctcatc tgctccttcc cactgctttc acctttcctc ggcccaggcg
     9361 cagttatcgc acaggttatc ggcgtattga ttggcgtgca gattggtctc ggcaatattc
     9421 cgccgcatct ggctttaccg gcactgtttg ccatcaacgc gcaggcggcc tgcgacttca
     9481 tcccggtcgg tttgtcgctg gcggaagccc gtcaggacac ggttcgcgtc ggtgtccctt
     9541 ctgtactggt gagccgcttt ttaaccggcg caccgactgt actgatcgcc tggtttgtct
     9601 ccggttttat ctatcaatag aggctgaaac atgaccgtta tttatcagac caccatcacc
     9661 cgtatcggcg cgagtgccac tgacgccctc agcgaccaga tgctcatcac ctttcgtgaa
     9721 ggcgcgccag cggacctcga agagtattgc ttcattcatt gccacggcga gttgaaaggt
     9781 gcactccatc ccggtttgca atttacgctc gggcagcatc gctatccggt gaccgctgtt
     9841 ggcagcgtgg cggaagacaa ccttcgcgaa ctgggtcatg tcaccctgcg cttcgatggt
     9901 ttaaacgaag cggaatttcc gggcactgtc catgtggcag gccctgtacc cgacgatatc
     9961 gcgccgggat cggttttgaa gtttgaatct gttaaggagt aaaaaatgaa tcaggttgcc
    10021 gttgtcatcg gtggtgggca aaccttaggc gcgttcctgt gccacggtct ggctgccgag
    10081 gggtatcgcg tcgcggttgt cgatattcag agcgacaaag ccgcaaatgt ggcacaagaa
    10141 attaacgccg aatatggtga aagtatggcg tacggttttg gtgctgacgc cactagcgag
    10201 caaagcgttc tggcgctctc tcgtggggta gatgaaatct ttggtcgcgt ggatttgctg
    10261 gtctatagcg ccggaatagc aaaagcagcc tttatcagcg acttccagct cggcgatttt
    10321 gaccgttcgc tacaagtgaa tctggtgggt tatttcctgt gtgcccgtga attttcgcgt
    10381 ttgatgatcc gcgacgggat tcagggtcgc attattcaga tcaactcgaa atccggcaaa
    10441 gtgggcagca aacacaactc tggctacagc gcagcgaaat ttggtggcgt cgggctgact
    10501 caatcactgg cgctggatct ggcggagtac ggcattacgg tgcattcact gatgctcggt
    10561 aacctgctga aatcgccgat gttccagtca ctgttgccac aatacgcgac caagctgggt
    10621 atcaaaccgg atcaagtcga gcagtattac atcgacaaag taccgctcaa acgcggctgc
    10681 gattatcaag atgtgctgaa tatgctgctg ttctacgcca gtcctaaggc gtcgtactgc
    10741 accggacagt cgatcaatgt caccggcggt caggtgatgt tctgatcaac agcggagatc
    10801 cattaaggat ctccgtgaga ctatagaatg cctgatgcgc tacgctcatc aggcatacag
    10861 gacttccgcc actacattaa ggaaaagtta tggtatccgc actcatcacc gtcgccgtta
    10921 tcgcctggtg tgcgcaactg gccttaggcg gctggcaaat ttctcgtttt aaccgtgcct
    10981 tcgacacact atgccagcaa gggcgggttg gcgtgggccg ttccagcggg cgctttaaac
    11041 cgcgggtcgt ggtcgccatc gcgctggacg atcagcagcg catcgtcgac accttgttta
    11101 tgaaaggact gaccgtcttc gcccgaccgc aaaaaattcc cgcaattacc ggtatgcatg
    11161 cgggtgattt acagcccgat gtgatctttc cccatgatcc actatcacag aatgctctat
    11221 cattggcgct taaactgaaa cgtggataat ttcgttgtga atgttacttg cttgcgaagt
    11281 tatcattttg aaacctaaat caggtaatca cgcccatgaa acctcgtcag cgtcaggccg
    11341 ccattctgga gtatctgcaa aagcagggta aatgctcggt tgaagaattg gcgcaatact
    11401 ttgacaccac aggcacaacc attcgcaaag atctggtcat tctggaacat gccggaaccg
    11461 tcattcgtac ttatggcgga gtggtgttga ataaagagga atccgatccg cctatcgatc
    11521 ataaaacact catcaacacc cacaagaaag agctgattgc agaagctgcc gttagtttta
    11581 tccatgatgg cgattcgatc attcttgatg ctggcagtac cgttttgcag atggttcccc
    11641 tgctctcgcg ctttaataac atcacggtga tgaccaacag cctgcatatc gtcaatgcgc
    11701 tatccgaact ggataacgaa caaactatcc tgatgccagg cggaacgttt cgtaaaaaat
    11761 cggcctcatt tcacggacag ctggcagaga atgccttcga acatttcagt ttcgataaat
    11821 tgtttatggg caccgacggc atcgatctca atgcgggcgt aaccaccttc aacgaggttt
    11881 ataccgtcag taaggcaatg tgcaatgccg cgcgcgaagt gattttgatg gcggactcat
    11941 caaagtttgg ccgtaaaagc cccaacgtag tttgcagtct tgaaagcgtc gataagctga
    12001 ttaccgacgc aggtatcgat ccggcgtttc gtcaggcgct ggaagagaaa gggatcgatg
    12061 tgatcataac cggagagagc aatgagtgaa gcactactga acgcgggacg tcagacgtta
    12121 atgctggagt tgcaggaagc aagccgttta ccggaacgtc tgggcgatga ttttgttcgc
    12181 gccgccaata tcatcctgca ctgcgaaggc aaagtggtgg tttcgggaat tggcaaatcg
    12241 ggccacattg gtaagaaaat cgccgcaaca cttgccagta ccggcacacc ggcttttttt
    12301 gtccatccgg cagaagcact gcacggcgat ctggggatga ttgaaagccg cgatgtgatg
    12361 ctgtttatct cttactccgg tggcgcgaag gaactggatc tgattattcc gcgtctggaa
    12421 gataaatcta tcgcgctgct ggcgatgacc ggcaaaccga cgtcaccgct gggcctggcg
    12481 gcaaaagcgg tgctggatat ctccgtagaa cgcgaagcct gcccgatgca ccttgcgccg
    12541 acctccagca ccgtcaatac cctgatgatg ggtgacgcgc tggcgatggc ggtcatgcag
    12601 gcgcgcggat ttaatgaaga agattttgcc cgctcccacc cagccggggc actgggcgct
    12661 cgcttgctga ataaagtgca tcatctgatg cgccgtgacg atgccatccc acaggtggcg
    12721 ttaaccgcca gcgtgatgga tgcgatgctg gaactcagcc gcaccggtct ggggctggtg
    12781 gcggtatgtg acgctcaaca acaggtacaa ggcgtcttta ccgacggcga tttacgtcgc
    12841 tggctggttg gcggcggcgc actcaccacg ccagttaatg aagcgatgac gacaggcggc
    12901 accacattac aggcacaaag tcgcgccatc gacgccaaag agatcctgat gaagcgcaaa
    12961 atcactgccg caccggtggt ggatgaaaac ggcaaactca ccggcgcaat taacctgcag
    13021 gatttctatc aggccgggat tatttaatcc ttcaatccca gacgtttcgc cagccgatgc
    13081 aggttggcga cgtcggtttc cagcatccgc gcgcaggcag cccagttgtg atgattttgt
    13141 gccagtgcct ggcgaatggt ttcacgctgg aacgcttctg tcgcttcacg caggttttgc
    13201 ttaacaacgg gcaccgccgc cgcttctggc ggcggcaacg tcacctcagg aaaagcaaaa
    13261 tgttgtgcct caagaatcac ttcatcgccg ctgcgagtgg ctctcgccag cactaccgcc
    13321 cgatgaatag catgttccag ttcgcgcacg ttccccggaa aacgatagtg ttgcagtaaa
    13381 tttcgcgctc cggcacttaa taccacgcgg gagagcccca gccgcaaacg acactgctcg
    13441 cagaaatacc ccgccagcag aatgacatca tcgccccgct cacgcagcgg cggcaccgaa
    13501 agtggaaaca cgctcaggcg atgaaacaaa tcggcgcgga atcgccctgc cagcacctct
    13561 tcgcgtaagt cgcggttagt cgccgccagc acgcgcacat cgacccgcaa actacggtca
    13621 tcgccaacgc gctgaatatc gccatactgc aacaccctca gcagcttggc ctgcaatgcc
    13681 aacgacaact cgccgatctc atcgagaaac agagtgccgt tatccgccat ttcaaacttc
    13741 ccgctgcggt tactgatagc gccagtaaac gctcctttca catgcccgaa caactcactt
    13801 tccgccacac tttccggcag tgcggcacag ttgagataga ccagcggatt caccgcccgt
    13861 ggcgaggctt catgaatcgc tttcgccacc agctccttac cggttcccgt ctcaccgctg
    13921 atcagaacgt tgagatcgga cgccgccaca atctcaatct cttttttcaa ttgcgtcatg
    13981 cctggcgaca agccaatcat ctgcgtctgt ttcaccgctt caaacggcgc ggcatcgccc
    14041 ggcagcatat tctggctttc cagttgttca atcagcaacg cattgcttaa cgctcccgcc
    14101 gccagcgcgg caatcagccg taactcttcg tcgctgaaaa catcgaactg atcgggctgc
    14161 atcccgtcga gcgtcagtgc gccgatcagg ttttgcccgg caaacaatgg cagaccaacg
    14221 caggcgtgaa ccttcagact ctcctgccca ggaatcaaac cgtcataggg atcgggcaat
    14281 tcgctgtctg caggaaagcg caccacgtcc ccggcgcggg caatcgcttc aagccgtgga
    14341 tgcccttcca gcgcaaagcg tctaccgagt acatcctttg ccagaccgtc gatggcaagc
    14401 ggaataaact gccgcgaatc gtaacgtagc aacgcagacg catcgcactc cagcacctga
    14461 cgtagcgtgg tgatcaggcg ctgaaaacga tcctgatgac caatcccacg ctgcaattcg
    14521 atggcgatat tcgccagcac atcaacggaa aaactcatct ttgcctcact gtcaatttga
    14581 ctatagatat tgtcatatcg accatttgat tgatagtcat tttgactact cattaatgag
    14641 cataatttta tttatagagt aaaaacaatc agataaaaaa ctggcacgca atctgcaatt
    14701 agcaagacat ctttttagaa cacgctgaat aaattgaggt tgctatgtct attgtggtga
    14761 aaaataacat tcattgggtt ggtcaacgtg actgggaagt gcgtgatttt cacggcacgg
    14821 aatataaaac gctgcgcggc agcagttaca acagctacct catccgcgaa gaaaaaaacg
    14881 tgctgatcga caccgtcgac cataaattca gccgcgaatt tgtgcagaac ctgcgtaatg
    14941 aaatcgatct ggcggatatc gattacatcg tgattaacca tgcagaagag gaccacgctg
    15001 gggcgctgac cgaactgatg gcgcaaattc ccgatacgcc gatctactgt actgccaacg
    15061 ctatcgactc gataaatggt catcaccatc atccggagtg gaattttaat gtggtgaaaa
    15121 ctggcgacac gctggatatc ggcaacggca aacagctcat ttttgtcgaa acaccaatgc
    15181 tgcactggcc ggacagcatg atgacttacc tgacaggcga cgcggtgctg ttcagtaacg
    15241 atgctttcgg tcagcactac tgcgacgagc atctgttcaa cgatgaagtg gatcagacgg
    15301 agcttttcga gcagtgccag cgttactacg ccaatatcct gacgccgttc agccgcctgg
    15361 taacgccgaa aattaccgag atcctgggct ttaacttacc agtcgatatg atagcgacct
    15421 cccacggcgt ggtctggcgc gataacccga cgcaaattgt cgagctgtac ctgaagtggg
    15481 ccgctgatta tcaggaagac agaatcacca ttttctacga caccatgtcg aataacaccc
    15541 gcatgatggc tgacgctatc gcccagggaa ttgcggaaac cgacccacgc gtggcggtga
    15601 aaattttcaa cgtcgcccga agcgataaaa acgaaatcct gaccaatgtc ttccgctcaa
    15661 aaggcgtgct ggtcggcact tcgacgatga ataacgtgat gatgccgaaa atcgccgggc
    15721 tggtggagga gatgactggt ttacgcttcc gtaacaaacg cgccagtgct ttcggctctc
    15781 acggctggag cggcggtgcg gtggatcgtc tttccacgcg cctgcaggat gcgggtttcg
    15841 aaatgtcgct tagcctgaaa gccaaatggc gaccagacca ggacgctctg gagttatgtc
    15901 gtgaacacgg tcgcgaaatc gcccgtcagt gggcgctcgc gccgctgccg cagagcacgg
    15961 tgaatacggt ggttaaagaa gaaacctctg ccaccacgac ggctgacctc ggcccacgga
    16021 tgcagtgcag cgtctgccag tggatttacg atccggcaaa aggcgagcca atgcaggacg
    16081 ttgcgccagg aacgccgtgg agtgaagttc cggataactt cctctgcccg gaatgctccc
    16141 tcggcaaaga cgtctttgaa gaactggcat cggaggcaaa atgagtaacg gcattgtgat
    16201 cattggttcg ggcttcgccg cccgccaact ggtgaaaaat attcgcaaac aggacgccac
    16261 tattccatta accctgattg ccgccgacag catggatgag tacaacaaac ctgacctcag
    16321 ccatgttatc agtcaggggc aacgtgccga cgaccttacc cgccagacgg cgggtgaatt
    16381 tgccgagcag tttaatctgc gcctgtttcc gcacacctgg gtgacggata tcgatgccga
    16441 agcccgtgtg gtgaaaagcc agaataatca gtggcaatac gacaagctag tgctggcaac
    16501 cggtgccagc gcctttgtcc cgccagtgcc tgggcgtgag ttaatgctga cgttaaatag
    16561 tcagcaagag tatcgcgcct gtgaaacgca actgcgggat gcccgacgcg tgttgattgt
    16621 cggtggtggc ttgattggta gcgaactggc gatggatttt tgtcgtgcag gcaaagcggt
    16681 cacgctaatc gacaacgctg ccagtattct ggcgtcatta atgccaccgg aagtaagcag
    16741 ccgcttgcag catcggttga cggagatggg cgttcatctg ttgttgaaat ctcagttgca
    16801 ggggctggaa aaaacagatt ctggcattct ggcaacgctg gaccgccagc gcagtatcga
    16861 agtggatgcg gtaattgccg ccaccggact gcgcccggaa accgccctgg cacgacgcgc
    16921 cgggctgacg attaatcgcg gcgtttgcgt cgatagttat ctgcaaacca gtaatgccga
    16981 tatttacgcg ctgggcgatt gcgcggaaat taacggtcag gtattgccgt tcctccagcc
    17041 gattcaactt agcgcgatgg tgctggcaaa aaatcttctc ggcaataaca cgccactgaa
    17101 actaccggcg atgctggtga aaatcaaaac gccggaatta ccgctgcatc tggcaggcga
    17161 aacccagcgt caggatttac gctggcagat taataccgaa cgccagggaa tggtggcgcg
    17221 cggcgttgac gatgctgacc agcttcgcgc ctttgtggtc agtgaggatc ggatgaaaga
    17281 ggcatttgga ttgttgaaaa cgttgccgat gtaggtgggc tactgtgcct aaaatgtcgg
    17341 atgcgacgct ggcgcgtctt atccaaccta cggggagcgc atgtgttggt cacatactgc
    17401 gttcatgtcg catccaccta ctgtgcccaa aatgtcggat gcgacgctgg cgcgtcttat
    17461 ccgacctacg gggacgcatg tgtaggccgg ataaggcgtt tacgccgcat ccggcaatgg
    17521 tgtccaaatg caacacgttt tatccgttct ggacttcacc cgctaaccaa cgcgccgcag
    17581 caataacccc ctgccccaga gacaaaccgc catcacccgc cggtaaactc tgtggaaaga
    17641 gcaatgtgaa atcagcgaga taatgcgcca gacgtgcacg cagcaaacgg ttatgaataa
    17701 ccccgccgct aaataccagc gtagtgatac cacgcatcgt ggcctgctca cgcatcaacg
    17761 cggcaaaacc ctgcgccagc gcatcatgaa acgcccacgc gcgttgatta accggtgcct
    17821 gccagttcag ccactgctgc cagaaagtgg cgagatccag ttgattgtcc accagcggca
    17881 ttgtcaccgg atgcgtcact ccgtggcacg aagctgcgag tgcctccaga gcacaagccg
    17941 cttcaccttc ataacttaac gtggctggcg cacagcccag tgccgccgcc acggcatcga
    18001 acaaccgccc acacgatgac gccagcggcg cgttaattcc acgctcaatg gcccgcgcca
    18061 gcacgctcca gttttgctgt tgcacacttg ctgtttcaga ataattctgc cactccggca
    18121 caaagcgcag gcactgcgcc agcaggtttc gccacggctg cttcgccgcc aaatcgccac
    18181 ccggaagcgc cactgcaggc aagccgccca ggtgctcaca ttcgcgatag ttcacccgca
    18241 gacactcgcc gccccacaaa gcgccgttct cccccatacc gataccatcg agcgtcaaag
    18301 caatgacatc cccgccatcc agcggccact gatgctctgc cagacacgcc gctgcgtggg
    18361 catgatgatg cagcaccgtt tgcgtcggca tattcatttc gctcgcccac tggctggaga
    18421 catagcccgg atgcacgtca tgcacaacgt attgcggagt gaaatcgtag atgttttgca
    18481 tcaggcgtaa cgcttcgcgc cactgcatct ggatgccatc gtcacttaaa tcgcccagat
    18541 gctgactcaa caccgcttgt tcaccgcgca ccaggcagaa ggtgtttttc agatccgcgc
    18601 cgagacacag cacaggcgga acatttttaa agcccggagg caaagccagc gcatccggca
    18661 catacccccg cgaacggcgc agcatttcgc cgctttcgcg caccaccgaa tcatccatcc
    18721 gctgcacgat gtcgcggtta tgtatcaaga atccgtcggc aatgccctgc aaatccgcca
    18781 gcgcctgttc gttgctgata gctggtggtt taccgctcag gttgccggag gtcatcacca
    18841 gcgggcattg cagttcctgt aacagcaaat gctggagcgg gttcgcaggc aacattaccc
    18901 cgacttcgtt aaggtcaggg gcgatatcat cacaaagctc aggaacgtat tttttatcca
    18961 ccagcacaat cggcgcggcg ggcgtggtaa gcaactggcg cgcagcgtct ggtaaaccgt
    19021 cagccactgg caacatgact gccagcggtt tcgccgggcg gtgtttgcgc tcccgcagtg
    19081 tcgccaccgc gttactgtta cgtgcatcgc aggcaagatg aaatccgcca atccctttga
    19141 tggcgacaat tttgcccatt tttaactgtg cgatagctgc ctgtaatgcc gcctcttgtt
    19201 ccgcatgttc accatgactt acccattcaa gatgcgggcc acactccggg caggccaccg
    19261 gctgggcgtg gaagcgacga tcgagcgggt cgcggtactc tttatcacag gctggacaca
    19321 gcggaaactc cgccattacg gtaaacgggc ggtcgtaagg catggcgcga ataatggtga
    19381 aacgcgggcc gcagtgggta cagttgataa acggataacg ataacgccgt tcgcctgggg
    19441 tattcatttc ggcaaggcaa gcagggcaag tagcggcatc cgggacaatt tgcgtattca
    19501 tggcacctcc cgcgctctgg cggatagtga actcggtggg cagttgtgac cagataaacg
    19561 gctcacgctc gacgctatca atacgcgcca gtggcggaca gtgctgatgc aattgaacaa
    19621 gaaacgtttc cgggtcttcc agcagccgga cttctacgcc atcgccgtca ttacagacat
    19681 cgccgtgaag atttaattgc tgtgccagct gccagacaaa cggacgaaaa ccgacgccct
    19741 gcactttgcc acgaatacgc agttggacac cgcaagatgt gttttttgcc attgagttat
    19801 tcccgccatc atgaattgcg taacccgtcc agccggacac gacagcgtcg catccggcag
    19861 tcacaggtcg gcgataccgc ccgcttcgta ttctacgaat atttccggga attcctttga
    19921 tgccagaaca gttctgtaag atttttagaa catcagcgcc gtacggcggc gtttttctgc
    19981 gctcagttgt tcaagtttat tacgatcgac acaaattagc gcatgggtcg ggcaagccgc
    20041 catacacgcc gggccgtctt cacgatggtt gcacaggtcg catttattgg cttcagcttt
    20101 gtcagcccgt acattcagcc ccacgccgct gttgcgaatc accggacgta ccaccacttc
    20161 catcgcgcca tacgggcaag ccaccacgca ggttttgcaa ccaatacaac gttcctgcat
    20221 cacatgaaca aaccctttat cacggctgat agcaccattc gggcagacgt tagcgcacgg
    20281 tgcatcttca cactgacggc aaactgtcgc cgtggaaatg ttcacacctt taatgacatg
    20341 gatacgcggt aaaaatgttt ccggggtcag cgatgcacag tcctgatttt cctgatgaga
    20401 aaccacgcac gccacttcac aggtacggca accaatacat ttactcgcgt cagcaatgat
    20461 gaaacggttc atcaaattct ccagcaatga cagttaatgc gccgatacat tcacaaatca
    20521 tgccagtttt taatttactg ttatttaagg aaattaattt ctgtaatgca ggaaaaacga
    20581 tgtcatcgac actagtgacg atgacatgtg atgacaatgt ttatcgcgaa agggcaatga
    20641 gtgagtcgcg gcagatcaat tttccgctga aggttttcgg cggtgagaaa tccccgccat
    20701 cgagcataaa aatcagccgt ccaataattt cctgaatcat ctcagtcacc ggaattttta
    20761 cgctggagag cgccggaacg atataggggg caattgcgat atcatcgaat ccgataactg
    20821 acacctgctc tggcaccgct acgccgcgct cgtgcaacgc tttcatcgca cctatcgcca
    20881 tatcgtcgtt actggcaact aacgcgctaa atttagcccc acgttcgagc aacatttcta
    20941 ccccttcggc cccgctggca ggcgtccatc taccgttagc gataagtttt tcattgagcg
    21001 caataccgtg ctgcgccagc gcgtctttat atccggcaag acgttcaacg ctggtgggag
    21061 aatccatcga accggtaagg aaagcaatct cctgatgtcc ggcgtttatc aactctgcca
    21121 cggcgttaaa actggtctgt ttatgatcgc accagacgct atggctgctg tttttgcgca
    21181 ggcggcgatt aagcaccatt atcggctgac tgtgcgcgtc aatgatgtca tcgatctcat
    21241 ccacgcttaa aaaacgcggg taaatcataa tggcgtcgca gcgcaaatcc agcagatact
    21301 gaatcgcctg gcgctcttct tcggcgctgt gtttaccatc tgccaatagc aactgccgcc
    21361 ctttctcttc cgccattcgc gcggcatgaa acagtaattc actaaaataa atgccgtggt
    21421 aaagcgtgtt ggtcactacc agccccagcg tctgagtact cttcgccgac aaattgcgcg
    21481 ccagcaagtt tgggcggtaa ccgctctctt ctaccgcctg aaacacgcga tctttagtct
    21541 cctggctgac gtagccatta cctgaaagca cgcgggaaac ggtcgctttt gaaaccccgg
    21601 cgcgcttcgc cacttccagc atcgtcgtca tcattttcat ccctttacac gcaatcaacg
    21661 cagtgtactg caccgtttgc cgattgtcct tgcgcaatcg gcgggaaaaa tattcaggtg
    21721 accggtttca caaatataaa aaatgaacaa ttaactctct tgcttattta gtgacaacta
    21781 ttcatgattt tgtgaaaccg gtttcctaat tccgtttcag catcggcatt tttccgttac
    21841 gtcgactgat aacaactaca tctaccctac tgataacagg ataaaatccg atggccaaaa
    21901 attatgcggc gctggcacgc tcggtgatag cggcactggg cggcgttgat aacatctcgg
    21961 cggtcacgca ctgtatgacg cgtttgcgct ttgttatcaa agatgaccag cttatcgaca
    22021 gcccgacgtt aaaaaccatc cccggcgtgc tcggcgtggt acgtagtgac aaccagtgtc
    22081 aggtgattat cggcaatacc gtttcacaag cctttcagga agtcgtcagc ctactgccgg
    22141 gagatatgca gcccgcacag cccgtgggta aacccaaact cacgctgcgt cgcattggtg
    22201 cagggatcct cgatgcgctg atcggcacca tgtcaccgct gatcccggcg attatcggcg
    22261 gatcgatggt caaactgctg gcgatgatcc tcgagatgag cggcgtgctg acaaaaggat
    22321 cgccgacctt aaccattctg aatgtgattg gtgacggtgc tttcttcttc ctgccgctga
    22381 tggtcgcggc atctgccgcc atcaaattta aaaccaatat gtcgctggcg attgccatcg
    22441 cgggtgtgct ggtacatccg agttttattg aactgatggc gaaagcggcc cagggtgaac
    22501 atgtcgaatt tgccctgatt ccggtcaccg cggtgaaata cacctacaca gtgatcccgg
    22561 cgctggtgat gacctggtgc ctgtcatata tcgaacgctg ggtggatcgc attacaccgg
    22621 cggtgaccaa aaacttcctc aagccaatgc tgattgtgtt gattgccgca ccgctggcaa
    22681 tcctgctgat tggcccgatt ggtatctgga tcggtagcgc catttcggcg ctggtttaca
    22741 ccattcatgg ttatctgggc tggctttcag tcgccattat gggcgcgctg tggcctctgc
    22801 tggtaatgac cgggatgcac cgcgtcttta cgccaaccat cattcagacc attgccgaaa
    22861 ccggcaaaga agggatggtc atgccgtcag agatcggcgc taacctgtcg ctgggcggtt
    22921 catcactggc ggtggcgtgg aaaacgaaaa acccggaact gcgccagacg gcgctggctg
    22981 cggcggcatc agccattatg gcggggattt ccgaaccggc gttatacggc gtggcgatcc
    23041 gcctgaaacg tccgcttatc gccagtctta tcagcggctt tatttgcggc gcggttgccg
    23101 gtatggcggg gcttgccagc cactcaatgg cagcgccggg gctatttacc agcgtgcagt
    23161 tcttcgatcc ggcgaatcca atgagcatcg tctgggtgtt cgcggtcatg gcgctggcgg
    23221 tcatgctgtc gtttatcctc acactgttgc tcggctttga ggatattcct gttgaggaag
    23281 cgactgccga ggcgcgaaag catcagagcg tacaaccgac cgtcgccaaa gaagtaagtc
    23341 ttaattgagg atgaaaatgt cagtatttcc agaaggtttt ttatggggcg gcgcgcttgc
    23401 cgccaaccag tctgaaggtg cgttccgtga aggtggcaaa gggctgacca ctgtcgatat
    23461 gatcccacac ggcgagcatc gaatggcggt gaaactgggg ctggaaaaac gttttcagtt
    23521 gcgcgatgac gagttttatc ccagccatga ggcgacggat ttttatcatc gttataaaga
    23581 agatatcgcc ctgatggcag agatgggatt caaggttttc cgtacctcaa ttacctggag
    23641 ccgtctcttt ccgcagggcg atgaaatcac gcccaatcag cagggcattg ctttttatcg
    23701 ttctgtcttt gaagagtgta aaaagtacgg tatcgaaccg ctggtcacgt tgtgccactt
    23761 cgatgtgccg atgcatctgg tcaccgaata tggctcctgg cgtaaccgca agctggtgga
    23821 gtttttcagc cgctacgccc ggacctgctt tgaagcattt gatggtctgg tgaaatactg
    23881 gcttaccttc aatgaaatca acattatgtt gcatagcccg ttctccggcg cgggtctggt
    23941 gtttgaagaa ggtgaaaatc aggatcaggt gaaatatcag gccgcgcatc accagctggt
    24001 tgccagtgcg ctagccacca aaatcgccca tgaggttaac ccgcaaaatc aggtgggctg
    24061 tatgctggcg ggcggtaact tctaccctta cagttgcaag ccggaagatg tctgggcggc
    24121 gctggagaaa gaccgggaaa acctgttttt tatcgatgtg caggcgcggg gcgcgtatcc
    24181 ggcttactct gcccgcgtat tccgcgaaaa aggggtaacc atcgacaaag caccgggcga
    24241 tgatgaaatc ctgaaaaaca ccgtcgattt tgtctctttc agctattacg cctcgcgctg
    24301 cgcctcggcg gagatgaacg ccaacaatag cagtgcggcg aacgtagtga aatcgctgcg
    24361 taacccgtat ctgcaagtga gcgactgggg ctggggtatt gatccactcg gtctgcgtat
    24421 caccatgaac atgatgtacg accgttatca gaagccgctg tttctggtgg aaaacggcct
    24481 cggcgcaaaa gatgaacttg ctgccaatgg cgagattaac gatgactatc gcatcagcta
    24541 tttacgcgaa catatccgcg caatgggcga agcgattgca gatggcattc cgctgatggg
    24601 ctacaccaca tggggctgta ttgatttagt ttccgcctct acgggtgaaa tgagcaaacg
    24661 ctacggtttt gtctacgtag accgtgacga cgcaggcaac ggcacgctga cgcgcacacg
    24721 taagaaatcg ttctggtggt ataaaaaagt gattgccagt aatggtgaag atttagagta
    24781 agcaacagtg ccggatgcgg cgtgaacgcc ttatccggcc tacatgtcgc acgaaccagg
    24841 aaggcccgat aagcgcagcg catcgggcaa tctggggttt ggcatcagtc aaaatcaaag
    24901 aaaccattcc ggcttaacgc caaatcgggc tgaaagtgcc ttaatgtggg atatcgtcag
    24961 ggatcgttgt ccagacaaaa tctgactgac cagggattta gagccgattt cgttcttcaa
    25021 atccgcatag gtcagattat gttgatcaat cagcgtgcga agtaacgcca cgccaacagg
    25081 cattgccgca acggctttgt cgaactctgc aaacttttcg ttgttgtttt catactctgc
    25141 aatgcggctc gccagaaagt cgataagtgg attggtgtca tcttcttcaa tcaaatattc
    25201 gaccagttcc agtgcttcac gataatcatc ttctgagtca ctgcctccca gaaaggggac
    25261 tgcattgacc agttctcttg ttgctttaac agcgcgtgca gcattagcag tcattctttg
    25321 ttctctctgt agtaacgtgt cagtttgtca tactctgcgt gattcgttat gtgcttcaca
    25381 aagaaccgct tattaacaaa gtttatgtag gcaataaccc taagattgtt cccgccgaca
    25441 tccagaacgt accatttgtt gcgatatcta aaattgtcga ggttgggaaa cgccgttcgc
    25501 atttcttctg gtgtagaaaa atccgtttcc ttaataaccc gatacaatgc ctgatgagcc
    25561 agagcgtcgt taggatattt acgcgcagac tcttcaaatg gcgctttaga aatgatgtgc
    25621 atttttaagt tttaacttcc ttatagtaag taaacagcca catctgcgat gttctatcct
    25681 tatgtgttcg gaaactatag ctcatcaaat ccaagttcac aatatgtaaa cacttgtgcg
    25741 cgatataatt acccccaaaa tcctcatagc cactctgcaa aaagtcagtt ctggttcacc
    25801 cctcaagcac atcccccttt atcaagcaac gctgcgtctg cgccagatct tccgtttgcg
    25861 ggtcgcgccc ggcgatgggt ttgaggacga gtttacggtt gggggtataa gtaaaacgcg
    25921 tgagcgcggg atagcttaac ttgccaaacc agccgtgaac acgctgccat ttggcgtctt
    25981 ttacctgata cagactggtt atccagtaac cagaattaaa ctgcatatcc agcaattgcg
    26041 tgtgaccatt gccttgcaaa tccagcaaat catctacgcc agttttgctt gcggtgtaga
    26101 atcccctggg ttcaaagaca cagggacggc cttcgcgcgt gaacgtcata agaattaaat
    26161 gtgcataagg agccagacca ttaccggtac ttggtaacca gataaccaga tcctgaatgc
    26221 cgttacgatc aagatcggcg cgatagagtt gccctttgcc cgctatgttt gccagcatac
    26281 ccacaggcac agtccaggat ttaccggcgc ggtctttagc ggttaattgc cagtaatctt
    26341 cttcatttgg catcttctca agcgtcacgc cagcaacgcc cactttccta aaatgccagg
    26401 tttgctcggc atcataaggc atggtcagga gaggtttcag cgcccgcttt tgcgcgtatt
    26461 cgctgtcact cattggcaga tattcggtaa agctggtggc ctgtgcaggc agcactaagc
    26521 tcaataaaaa agcgcacatc cctgttctga tgttcaattc cctgaattcc ctgatggaga
    26581 attattcttc ttccaccgct aactgcgcaa atccaccatt tccttcccag ccttccagtc
    26641 gttgataaac ggtttctacc gcatctttaa tcggctgggt catcgggtag taaaagccga
    26701 cgatatccgg ctgaatgccg aggaaaatca cttcaccaat gtcttctttt aactggtcga
    26761 taaggtaatt caacggcatg ttatgggtag tcatcataaa catctcggcg atatcatccg
    26821 ggtcgatgat gcggatttcg ccggggttta gccccatatc cgtggcgtcg acaatcagca
    26881 gtcgtgtcgg gcgcagttca cggatggcga cgatgtcgtt ttccggtgca ctaccgccgt
    26941 caatcaccac ccagttacct ttcggcgcgg cggcgcactt ttccgccagc agcgggccag
    27001 cgccatcatc gcccatcatg ctattgccaa cacagagtaa aacgtcagtc acgcagtctc
    27061 ctcaccatca aatagatggc gttttcctga tgaatatcat gcagcatact taacagcatt
    27121 gtactccatt ccttctgctg cgaagtttgc gctgcccggg ctttatcgaa tgcgttggcc
    27181 agcatcggca catggttaat gtcgatgacg atctcgccat atttcggtac gccttccatt
    27241 ttgcggcggg cttcgctgcc cgcctccaga gtggcaatcc atgccagata ttcatcccat
    27301 gggcaggtga gcgccgcttc caggcaatcg ataaccccaa ggtggtgacc aatcgccagg
    27361 ctgtaataga ccacctgctg cgcctcggcg ggcgtggcat cgttctcatc aataaattta
    27421 cggctcagtt gactgaacac caccttttca ctcatcggat acgcgcctct tcaacaacat
    27481 gattcagatg gctgacaatc tcgttcagac gcggatcgtt ttccgcttcc agccagcgtg
    27541 caacctgttc ttcgccctgc cctaactgtg taaggtaatc atcggcaatc tgacgaccgt
    27601 aacgataacc cgccaggcga cgtgcttcgc gatccacttt cacgcgcagc ggctgcacca
    27661 tatcgccatg cagaatctca gccggttgtt catcctgctc acccgggcca cgggcgtgaa
    27721 ttttctgctc cagcaggccg agtgccattg caaagccgta cagcgtggcg gcaggcgttg
    27781 gcgggcaacc cggaatgtag acatctaccg ggacgatttt atccgtaccg ccccacacgc
    27841 agtagagatc gtggaaaata ccaccgctgt taccgcaggc accgtaggag atacaaattt
    27901 tcgggtccgg cgcggactgc cacgcacgca gcgcagggga tcgcattgca cgggtgaccg
    27961 cgccggtaaa cagtaaaata tccgcatgac gcggtgaagg aacgacttta atgccgaagc
    28021 gttctgcatc aaacagcggc gataaagtgg cgaaaatttc gatttcgcag ccgttgcagc
    28081 cgccgcagtc cacgcggtaa acataggcag aacgtttgat ttttttcagt aacgacgcct
    28141 tcatgctggc gatggattca tccaccgtca tcgggaccgg aatgccgttg gcgtcacggg
    28201 ggcctaataa attgctcatc agatggcctc tttcatatgg cgagtcagtt caatacggtc
    28261 ggacggcacc aggcatttct ggcgcttaca ttccgggcaa gtctcaaagc tttcgcggtg
    28321 gttttccgcg cggctgtcac cattgtgctt aagcagcgca atggcgtagt cgatctcttt
    28381 ctggacggcg aaaggacgat tgcagacgcg acagctgcac agcacaaaac gggactgttg
    28441 caggaaatct tctttcttcc acaccgccag ttcgtactct tgcgacagtt tgatcgctgc
    28501 cgtcgggcag acttcttcgc agcgtccaca gaagatgcag cgcccgagat taaactccca
    28561 cgccagctca ccggtggcga ggtcagtttc aaccgttaag gcgtttgacg ggcaggcatt
    28621 gacgcaggcc gcacagccga tgcactgctg cgggttctgc tccggcttac cacggaagtt
    28681 tttatcaacc gcaatcggct ccagcggata agacgaggtc gccgtgccgg ttttgatgac
    28741 ttttttgata aaggtaaaca tggcgattcc ttatttcagc ggcgagtttt tacgctcaat
    28801 gctgtaacgc tcgagttctt tgtacggcac cactttgctc tttttcttac gcacatcgac
    28861 cacggtcatg cggtcggtac aggagtagca agggtccagg ctaccgataa tcagcggcgc
    28921 atcggaaacg gtgttaccgc gcagcatgta gcgcagggtc ggccagttcg cgtaggtcgc
    28981 ggcacggcag cgccagcggt acagcttctg gttgtcgccg gtcatgctcc agtggatatc
    29041 gtcgccgcgc ggcgcttcgg caaagcccag cgcgaaacgg tgcggaatgt aggtaaagcc
    29101 ttccaccatc agcggaccgc ccggcaggtt atccagaccg tagtcgatca tgttcagcgc
    29161 ggtataaact tcgttgatac gcactttcag acgggaaata acgtcgcagc cctgctcgct
    29221 gtggacttcc attggcagca ggccataacc gacaaacggg tgatcggcgc gagtatcgcg
    29281 ggcgtgaccg ctggcgcgaa ccatcgggcc aacgttactg aaatcgcggg cgatttccgg
    29341 gtccagacga ccaatgccga cggtgcgctg ttccatgttc ggcgtactca gcagcacatc
    29401 caccagctcc tgcacttcac gacgcatctg ttgcgccagc tggcgggtct ggatcatgtc
    29461 atctttcaac agatcgcgac gaatcccgcc gatcaggttc aggccgtagg ttttacgcgc
    29521 accggtgagg atctctgcca ttttcatgga ggtttcacgc acgcggaaga actgcataaa
    29581 gccggagtca aagccggtaa agtggcaggc gagaccgagg ttgagcagat gcgagtgcag
    29641 gcgttccacc tccagcagaa tggcgcggat catctgcgca cgttctggca cctgaatacc
    29701 catcgcgttt tctaccgacg tggtgtaggc ggtgctgtgg gcaaagccgc agatcccgca
    29761 cacacggtca gagaggaagg tcacttcgtt gtaacccata cgggtttccg ccagtttttc
    29821 catgccgcga tgaacgtaga acagacggta gtcggcgtcg ataatgtttt cgccatcgac
    29881 gaacagacgg aagtggcccg gttcgtcaga agtgacgtgc agcggaccaa tcggcacgac
    29941 gttgtttttc ttgtcgccca gttcgttgat gaactcgtag gtttcagcat cggtagtcgg
    30001 tgccggacgc tgacgataat ccatgctgtc tttacgcagc ggataaagtt catccggcca
    30061 gtcatccggc agcaccagac gacgttcatc cggcagacca accggaatca aaccgtacat
    30121 atcgcgcact tcgcgctcgc cccacaccgc cgccggaacg cgcggcgtca cggacggata
    30181 ctccggtttg ttggcgtcaa cttcgacgcg aaccgttacc caacacttgg tgcccttctc
    30241 catcgacagc acgtaataaa cggcgtaatg accattcagt ttgcgttcgt cgttaccaaa
    30301 cagcaccgac agccagccac cctgcttgta gtagagaaac tccaccactt ccggcaggta
    30361 gttcaccttc acggtcacgg tcagctgatc tttggtctgc caggcgtggt ccagcacgac
    30421 gcccggaaat gcctcattca gcgcggcgag ataatgttga cctaattttt cttcagacat
    30481 gctcaaactc tctttaatca cgccgccagc aaggagacga acgctaaaaa tgcaaagcca
    30541 aacccagccc aggtaatgcg cggagtaata tcaagacgca gacgggccat gctgttttcg
    30601 aacagcgcga taaccaggac gccgacgacc agtttgacga tggcaatcac cagcgccagc
    30661 agcagcccac cggcggtgaa ggtttccatc tgtccccacg ggataaatac gccgacgaac
    30721 atctgcagca ccaccagctg tttcaggctg ataccccatt tcatgacgcc aaagccgctg
    30781 ccgctgtatt cagagagcgg gccttcctgc agctcctgct cggcttccgc caggtcgaac
    30841 ggcagtttgc ccatttcgat aaaggtggcg aacgcacagg cacaaagcgc cagtaccagc
    30901 gggatgctct ggctcagcgg ccagtgataa acggtgtcgg tgatgttgct gatgttggtg
    30961 gaaccggcaa cctgtgcggc aacccacaga ccaagcagca gcatcggttc gaccagcacg
    31021 ccaagcatcg cttcacggct cgcgccgata gcggtaaacg ggctaccggt atccagacca
    31081 gaaatggcaa agaagaaacg cgcgatggca aagagataca gtaaggtgat taaatcaccc
    31141 agttgcggca gcggagaacc gacggtcacc accggcagcg cagtagcgat agtcagcatg
    31201 acgcccacca tcacatacgg cgtcaggcgg aacacccagc cggaggcatc cgggccaacg
    31261 ctctggcggc ccagcagttt gataatgtca cgatactcct gcaacacacc cggcccacga
    31321 cggttatgta agcgggctcg cgccacgcgg gtaataccgg agagcagcgg cgcaacggca
    31381 aatagcacca gcgcctgaat taacggatat aaaacactca ttctcaggct cctcgtgaaa
    31441 caataatcac caccagtacc gccagttcaa ccagcgccat ccggcggaac agcaacgcac
    31501 tcccctcgca ctgccagccc ggcaccagag acaccggatt cagccatttg cgtagtttca
    31561 gcaccggcgc aaacgcctgt ttcaccggca tggcaaaacc gtgagcggta atcaccattg
    31621 atttttcgtg gtcgtagccg cacacccagg ccgcgccgcg ggaacgcgat ggcaaacgat
    31681 cgcctttgca aatcgccata atgatgaatg gcagcagcgg acaggcaatc agcagcaatg
    31741 tgatcattgg ttgagaaacg gtggtgttag caggctccag cggcagaggt acagcagcag
    31801 acagcatcgg cagcagccac ggcgcagcaa caccgccaat tacgcagcaa atcgccagtg
    31861 ccactacgct taccctcatc aggagcggcg cacaggtggc gttttcggct tctttggtac
    31921 gcggcgcgcc gaggaacgtg acgccataga ctttcgccat acacatcacc gccagcgcac
    31981 cggtaattgc cagacccaca gcgagcagcg gccccagcag acgggcaaca aacgcgccgc
    32041 tattgctcag tttgaagaag gattgataga taacccattc cccggcaaac ccgttcagcg
    32101 gcggcagcgc agccattgcc attaacccga ctaacatggc gatggagata accggcattt
    32161 tcttgccaat accaccgagt ttttcgatat cgcgatgacc ggtacggaac cagacgctcc
    32221 ccgccccgag gaacagtacg cttttgaaca ggctatggtt aagcagatgg tacagaccac
    32281 cgaccaggcc aagagcaatc agcgccggtt gttcgagcgc gatacccgtt acgccagcgc
    32341 ccagccccag caggataatg ccgatatttt ccagggtgtg gtaagccagc aggcgctgga
    32401 tgttgtgctc catcagcgca tacagaccac cgacaaacgc ggtgatcatg ccgagcacca
    32461 gcagcgcgat cccccaccac agcggtgcat taccgcccag cagtgacagg gttaaaatgc
    32521 ccagcaggcc aattttcatg actaccgtag aaaacaacgc ggcagctggc gcagaggcgt
    32581 tagcatgtgc ctgcggcacc cagccgtgca gcggaataat cccggcgagc aggccaaagc
    32641 caatcacacc gagcagccag atatcggaac cgagcggcag ctgttgcata cgcatatcca
    32701 gcaggcgcag atccagcgtg ccataacgct gccacagcag ccagcaagca atcgccagca
    32761 gcagagtgcc aagacgcccc agcgcaaacc acagtttgcc ctctttgctg ttgctagtga
    32821 ggaacaccgc gcacagggcc atgatttcgg ccattaccac gaacatgccg aggttgctgg
    32881 caatgacggc gcagacggcg gcagccatca acatattgat ctgcaagccg ttgcacttca
    32941 cctgcgcgtg gcgatgccag tcaatgttat agaggctgac aaacagaccg caaaggccga
    33001 gggtaatcag ccaaatcgcg ttaagcgggg agatttgcac atcgtagctt accagcgaca
    33061 gcgcaccgct cacgccaacc gcgccagtca gcacagtgaa gcccgcggct gccgtataca
    33121 ggctaccaac cgcgccgcca attccggcta tccagccact tagcgtcttt tgaaaagaaa
    33181 agagaaatgc cagaacagcg gcggcgacaa accacgccac gccgctattg atcagggaaa
    33241 ttgcgctcat ttagcctctc cactttgagc ctgctgaaac aaggtgagat cgccaaagtc
    33301 cgtgttaaag gtcagctcac gcttacgttt gctgacgcgg gcgatatcgg tgttatccac
    33361 cagatgcagg gctttagtcg ggcacatccg cacgcaggcc ggaccttgtt catcaaagct
    33421 acaaaggtca catttgacgg cgatcgcgcg aatacctggc acccagtcaa gcaatgtgct
    33481 gacacgcgcc ggagcaggcg gtgccggtgg cgctttcggg gtattggcgt ttgccggaat
    33541 atccagcgga cggctgccgg aaaattcaat tgcgccaaac gggcaggcga tgccgcacag
    33601 cttgcagctt acgcacaggc tttcattcaa ctgcacggcc ccatcaacgc gggtgatggc
    33661 gttaaccggg cagaccaccg cgcagggtgc atcttcacag tggtgacaga gctgcggcgc
    33721 agattctttt tcattcagca tcactctcag gcgcggcatt gattgcaggc cgtgctggcg
    33781 atgcgtctct gaacaggcgg cctcacaagt gtggcagccg atacagagcg tggagtcagc
    33841 aattacaaaa cgattcacca ggcattcctc aggtgattgt catttttgac gaaaacatgc
    33901 cgatgaaatg tcattttcga cactcatcga cacgcccatc cccaaacagg cgtaacgcct
    33961 gcaaaacggg caaagcctca gctcatgctg ccgggctttg tccctttacc agttggctta
    34021 aattcaccgg cacattgttt tcaatagcgg tgtataagct gtcgtaaacg ccagcgattt
    34081 tttcgagata gtgttccaga acctgttcgc gatcgcggat gttgaccttg ccatcaatca
    34141 tgccgtcaat gctcagttgc gcctgcgcaa tccgctgttt gtcttcgcca tcttttgttt
    34201 cgacgtaata ctcgatggtg tggctgttaa agccatccgc cagggtgacg taaatgcgaa
    34261 aatgttcaaa aaggacgaaa cagagttctt tgtccggcgc gcagctcatg gcgtgatgca
    34321 ggcgcgcttt cgataacgtg atcccctgaa ccagcgaatt gcagtagatg tgccactggt
    34381 cctgtaggcg acgatgccgc tgtgcgatgt aatcggcttt ctcgcttatt tcccaaatag
    34441 tcattgtcag gttacccgtt taacagagat gccagcttta agcattttct gtgccaactt
    34501 ttaatttatt gttattaaag agatttttaa gctaaagatg aatttcgtcg ccgtgtcgac
    34561 gtgtcatttc gacatcatcg acattattca ccgcagggat aatcaacact ggcacaatta
    34621 ttgcttgtag ctggcaatag ttaatgggag gcgatatgca cgaaataacc ctctgccaac
    34681 gggcactgga attgatcgaa cagcaggccg caaaacacgg cgcaaaacgc gtaactgggg
    34741 tctggctcaa aattggcgca ttttcttgtg tcgaaaccag ctctcttgcc ttttgttttg
    34801 atctggtttg ccgcggcagc gtggcggaag gttgtaaact gcacctcgaa gaacaagagg
    34861 ccgaatgctg gtgtgaaaca tgccaacagt atgtgacgct actgacccag cgcgtccgcc
    34921 gctgtccaca gtgtcatggt gacatgctgc agattgtggc agacgacggt ttacagattc
    34981 ggcggataga aatagaccag gagtgagcga tgtgtacaac atgcggttgc ggtgaaggca
    35041 acctgtatat cgagggtgat gaacataacc ctcattccgc gtttcgtagc gcgccatttg
    35101 ccccggcggc acgcccgaag atgaaaatca ccggcattaa agcgcctgaa tttaccccca
    35161 gccagactga agaaggcgac ctgcattacg gtcatggcga agcgggcact cacgcaccgg
    35221 gcatgagcca gcgtcggatg ctggaagtcg aaattgacgt gctggacaaa aataaccgtc
    35281 tggctgaacg caaccgcgcg cgctttgctg cccgcaagca actggtgctc aacctggttt
    35341 ccagcccagg ttccggtaaa accaccctgc tgacggaaac cttaatgcgc ctgaaagaca
    35401 gcgttccgtg cgcggttatt gaaggtgacc agcaaaccgt gaacgatgcc gcacgcattc
    35461 gcgctaccgg cacaccagcg attcaggtga acaccggtaa aggctgccat cttgacgcac
    35521 agatgattgc cgacgccgca ccgcgtctgc cactggacga taacggtatt ctgtttatcg
    35581 aaaacgttgg caacctcgta tgcccggcca gcttcgatct cggtgaaaaa cacaaagtgg
    35641 cggtgctttc cgttaccgaa ggtgaagaca aaccgctgaa atatccgcat atgtttgccg
    35701 ccgcctcgct gatgctgctc aacaaagttg acctgttgcc gtatctcaac tttgacgttg
    35761 agaagtgcct cgcctgcgcc cgcgaagtca atccagaaat tgaaatcatc cttatttccg
    35821 ccaccagcgg tgaagggatg gaccagtggc tgaactggct ggagacacag cgatgtgcat
    35881 aggcgttccc ggccagatcc gcaccattga cggcaaccag gcaaaagtcg acgtctgcgg
    35941 cattcagcgc gatgtcgatt taacgttagt cggcagctgc gatgaaaacg gtcagccgcg
    36001 cgtgggccag tgggtactgg tacacgttgg ctttgccatg agcgtaatta atgaagccga
    36061 agcacgcgac actctcgacg ccttacaaaa catgtttgac gttgagccgg atgtcggcgc
    36121 gctgttgtat ggcgaggaaa aataatgcgt tttgttgatg aatatcgcgc gccggaacag
    36181 gtgatgcagt taattgagca tctgcgcgaa cgtgcttcac atctctctta caccgccgaa
    36241 cgccctctgc ggattatgga agtgtgcggt ggtcataccc acgctatttt taaattcggc
    36301 ctcgaccagt tactgcctga aaacgttgag tttatccacg gtccgggttg cccggtgtgc
    36361 gtactgccga tgggtagaat cgacacctgc gtggagattg ccagccatcc ggaagtcatc
    36421 ttctgtacct ttggcgacgc catgcgcgta ccagggaaac agggatcgct gttgcaggca
    36481 aaagcacgcg gtgccgatgt gcgcatcgtt tactcgccga tggatgcgtt gaaactggcg
    36541 caggagaatc caacccgcaa agtggtgttc ttcggcttag gttttgaaac caccatgccg
    36601 accaccgcca tcactctgca acaggcgaaa gcgcgtgatg tgcagaattt ttacttcttc
    36661 tgccagcaca ttacgcttat cccgacgctg cgcagtttgc tggaacagcc ggataacggt
    36721 atcgacgcgt tcctcgcgcc gggccacgtt agtatggtta tcggcactga tgcctataat
    36781 tttatcgcca gcgattttca tcgtccgctg gtggtcgctg gtttcgaacc ccttgatcta
    36841 ctgcaaggcg tggtcatgct ggtggagcag aaaatagcgg cccacagcaa ggtagagaat
    36901 cagtatcgtc gggtggtacc ggatgccggt aacctgctgg cgcaacaggc gattgccgat
    36961 gtgttctgtg tcaacggcga cagcgaatgg cgcggcttag gcgtgattga atcttctggc
    37021 gtgcacctga cgccggatta tcaacgattc gatgccgaag cacatttccg cccggcaccg
    37081 cagcaggtct gcgatgaccc gcgcgcgcgt tgtggtgagg tattaacggg caaatgtaag
    37141 ccgcatcaat gcccgctgtt tggtaacacc tgtaatcctc aaaccgcatt tggtgcactg
    37201 atggtctcct ccgaaggagc gtgcgccgcg tggtatcagt atcgtcagca ggagagtgaa
    37261 gcgtgaataa tatccaactc gcccacggca gcggcggcca ggcgatgcag caattaatca
    37321 acagcctgtt tatggaagcc tttgccaacc cgtggctggc agagcaggaa gatcaggcac
    37381 gtcttgatct ggcgcagctg gtagcagaag gcgatcgtct ggcgttctcc accgacagtt
    37441 acgtcattga cccgctgttc tttcctggcg gtaatatcgg caagctggcg atttgcggca
    37501 ctgcgaatga cgttgcggtc agtggcgcta ttccgcgcta tctctcctgt ggctttatcc
    37561 tcgaagaagg attgccgatg gagacactga aagccgtagt gaccagcatg gcagaaaccg
    37621 cccgcacggc aggcattgcc atcgttactg gcgatactaa agtggtgcag cgcggcgcgg
    37681 cagataaact gtttatcaac accgctggca tgggcgcaat tccggcgaat attcactggg
    37741 gcgcacagac gctaaccgca ggcgatgtat tactggttag cggcacgctc ggcgaccacg
    37801 gggcgactat ccttaacctg cgtgagcagc tggggctgga tggcgaactg gtcagcgact
    37861 gcgcggtact gacgccgctt attcagacgc tgcgtgacat tcccggcgtg aaagcgctgc
    37921 gtgatgccac ccgtggtggt gtgaacgcgg tagttcatga gttcgcggca gcctgcggtt
    37981 gtggtattga aatttcagaa gcggcactgc cggttaaacc tgccgtgcgc ggcgtttgcg
    38041 aactgctggg actggacgcc cttaactttg ccaacgaagg caaactggtg atcgccgttg
    38101 aacgcaacgc ggcagagcaa gtgctggcag cgttacattc ccatccactg ggaaaagacg
    38161 cggcgctgat tggtgaagtg gtggaacgta aaggtgttcg tcttgccggt ctgtatggcg
    38221 tgaaacgaac cctcgattta ccacacgccg aaccgcttcc gcgtatatgc taataaaatt
    38281 ctaaatctcc tatagttagt caatgacctt ttgcaccgct ttgcggtgct ttcctggaag
    38341 aacaaaatgt catatacacc gatgagtgat ctcggacagc aagggttgtt cgacatcact
    38401 cggacactat tgcagcagcc cgatctggcc tcgctgtgtg aggctctttc gcaactggta
    38461 aagcgttctg cgctcgccga caacgcggct attgtgttgt ggcaagcgca gactcaacgt
    38521 gcgtcttatt acgcatcgcg tgaaaaagac acccccatta aatatgaaga cgaaactgtt
    38581 ctggcacacg gtccggtacg ccgcattttg tcgcgccctg atacgctgca ttgcagttac
    38641 gaagaatttt gtgaaacctg gccgcagctg gtcgcaggtg ggctataccc caaatttggt
    38701 cactattgcc tgatgccact ggcggcggaa gggcatattt ttggtggctg tgaatttatt
    38761 cgttatgacg atcgtccctg gagcgaaaaa gagttcaatc gtctgcaaac atttacgcag
    38821 atcgtttctg tcgtcaccga acaaatccag agccgcgtcg tcaacaatgt cgactatgag
    38881 ttgttatgcc gggagcgcga taacttccgc atcctggttg ccattaccaa cgcggtgctt
    38941 tcccgcctgg atatggacga actggtcagc gaagtcgcca aagaaatcca ttactatttc
    39001 gacattgacg atatcagcat tgtcttacgc agccaccgta aaaacaaact caacatctac
    39061 tccactcact atcttgataa acaacatccc gcccacgaac agagcgaagt cgatgaagcc
    39121 ggaaccctca ccgaacgcgt gttcaaaagt aaagagatgc tgctgatcaa tctccacgag
    39181 cgggacgatt tagcccccta tgaacgcatg ttgttcgaca cctggggcaa ccagattcaa
    39241 accttgtgcc tgttaccgct gatgtctggc gacaccatgc tgggcgtgct gaaactggcg
    39301 caatgcgaag agaaagtgtt taccactacc aatctgaatt tgctgcgcca gattgccgaa
    39361 cgtgtggcaa tcgctgtcga taacgccctc gcctatcagg aaatccatcg tctgaaagaa
    39421 cggctggttg atgaaaacct cgccctgacc gagcagctca acaatgttga tagtgaattt
    39481 ggcgagatta ttggccgcag cgaagccatg tacagcgtgc ttaaacaagt tgaaatggtg
    39541 gcgcaaagtg acagtaccgt gctgatcctc ggtgaaactg gcacgggtaa agagctgatt
    39601 gcccgcgcga tccataatct cagtgggcgt aataatcgcc gcatggtcaa aatgaactgc
    39661 gcggcgatgc ctgccggatt gctggaaagc gatctgtttg gtcatgagcg tggggctttt
    39721 accggtgcca gcgcccagcg cattggtcgt tttgaactgg cggataaaag ctctctgttc
    39781 ctcgacgaag tgggcgatat gccactggag ttacagccga agttgctgcg tgtattgcag
    39841 gaacaggagt ttgaacgtct cggcagcaac aaaatcattc agacggacgt acgtttaatt
    39901 gccgcgacta accgcgatct gaaaaaaatg gtcgccgacc gagagttccg tagcgatctc
    39961 tattaccgcc tgaacgtatt cccgattcac ctgccgccac tacgcgagcg tccggaagat
    40021 attccgctgc tggcgaaagc ctttaccttc aaaattgccc gtcgtctggg gcgcaatatc
    40081 gacagcattc ctgccgagac gctgcgcacc ttgagcaaca tggagtggcc gggtaacgta
    40141 cgcgaactgg aaaacgtcat tgagcgcgcg gtattgctaa cacgcggtaa cgtgctacag
    40201 ctgtcattgc cagatattgt tttaccggaa cctgaaacgc cgcctgccgc aacggttgtc
    40261 gcccaggagg gcgaagatga atatcagttg attgtgcgcg tgctgaaaga aaccaacggc
    40321 gtggttgccg ggcctaaagg cgctgcgcaa cgtctggggc tgaaacgcac gaccctgctg
    40381 tcacggatga agcggctggg aattgataaa tcggcattga tttaactgca aattgccgga
    40441 cagatctgcc tgtccggcat actattcacg aggttttttc ggacgatatt tttccggcag
    40501 ttctggcacc ggacgcttgt catcgatgag atgacgcacg gttaagatcg gatgacgcca
    40561 cagcattctc ggcccggccc aacgcataat ctgtttcatc tcttcacgct ttgcaggctg
    40621 gtaacagtgc accggacact gcttacaggc tggtttctct tcgccgaaca cacatttatc
    40681 cagccgcttt tgcgcgtaaa caaacaacgc ctcgtaatgc tccggctccg ctgacgcctg
    40741 cgggcatttc gcttgataaa gatcgatcat ttttttaatc gtcagttttt cacgagagat
    40801 acgcttgccg gacatgctgc ctccatactc ttaaggtgta tttatattac aacttaattt
    40861 gaaaggggac tatggctctg aaggaggagg tttattcaac cgacgcaatt ttgcgtactt
    40921 gcttcataag catcacgcaa aagctgcaaa acagcatctt tcccggaacc agcatcaaga
    40981 actcgcagtt cgcttcttcc cctgaaatga ttaactccgg tatcatgtgc gccttatgtg
    41041 attacaacga aaataaaaac catcacaccc catttaatat cagggaaccg gacataaccc
    41101 catgagtgca atagaaaatt tcgacgccca tacgcccatg atgcagcagt atctcaggct
    41161 gaaagcccag catcccgaga tcctgctgtt ttaccggatg ggtgattttt atgaactgtt
    41221 ttatgacgac gcaaaacgcg cgtcgcaact gctggatatt tcactgacca aacgcggtgc
    41281 ttcggcggga gagccgatcc cgatggcggg gattccctac catgcggtgg aaaactatct
    41341 cgccaaactg gtgaatcagg gagagtccgt tgccatctgc gaacaaattg gcgatccggc
    41401 gaccagcaaa ggtccggttg agcgcaaagt tgtgcgtatc gttacgccag gcaccatcag
    41461 cgatgaagcc ctgttacagg agcgtcagga caacctgctg gcggctatct ggcaggacag
    41521 caaaggtttc ggctacgcga cgctggatat cagctccggg cgttttcgcc tgagcgaacc
    41581 ggccgaccgc gaaacgatgg cggcagagct gcaacgcact aatccggcgg aactgctgta
    41641 tgcagaagat ttcgccgaga tgtcgctgat tgaaggccgt cgcggcctgc gccgtcgccc
    41701 gctgtgggag tttgaaatcg acaccgcgcg ccagcagttg aatctgcaat ttggcacccg
    41761 cgatctggtc ggttttggcg tcgagaacgc gccgcgcgga ctttgtgctg ccggttgtct
    41821 gttgcagtat gcgaaagata cccaacgcac gaccctgccg catattcgtt ctatcaccat
    41881 ggaacgtgag caggacagca tcattatgga tgccgcgaca cgtcgtaacc tggaaattac
    41941 tcagaacctg gcgggcggtg cggaaaatac gctggcttct gtgctcgact gcaccgtcac
    42001 gccgatgggt agtcgtatgc tgaaacgctg gctgcatatg ccagtgcgcg atacccgcgt
    42061 gttgcttgag cgccagcaaa ctattggcgc attgcaggat ttcaccgccg agttgcagcc
    42121 ggtactgcgt caggtcggcg acctggaacg tattctggca cgtctggctt tacgaactgc
    42181 tcgcccacgc gatctggccc gtatgcgcca cgctttccag caactgccgg agctgcgtgc
    42241 gcagttagaa actgtcgata gtgcaccggt acaggcgcta cgtgagaaga tgggcgagtt
    42301 tgccgagctg cgcgatctgc tggagcgagc aatcatcgac acaccgccag tgctggtacg
    42361 cgacggtggt gttatcgcat cgggctataa cgaagagctg gatgagtggc gcgcgctggc
    42421 tgacggcgcg accgattatc tggagcgtct ggaagtccgc gagcgtgaac gtaccggcct
    42481 ggacacgctg aaagttggct ttaatgcggt gcacggctac tacattcaaa tcagccgtgg
    42541 gcaaagccat ctggcaccca tcaactacat gcgtcgccag acgctgaaaa acgccgagcg
    42601 ctacatcatt ccagagctaa aagagtacga agataaagtt ctcacctcaa aaggcaaagc
    42661 actggcactg gaaaaacagc tttatgaaga gctgttcgac ctgctgttgc cgcatctgga
    42721 agcgttgcaa cagagcgcga gcgcgctggc ggaactcgac gtgctggtta acctggcgga
    42781 acgggcctat accctgaact acacctgccc gaccttcatt gataaaccgg gcattcgcat
    42841 taccgaaggt cgccatccgg tagttgaaca agtactgaat gagccattta tcgctaaccc
    42901 gctgaatctg tcgccgcagc gccgtatgtt gattattacc ggcccgaaca tgggcggtaa
    42961 aagtacctat atgcgccaga ccgcactgat tgcgctgatg gcctacatcg gcagctatgt
    43021 accggcacaa aaagtcgaga ttggacctat cgatcgcatc tttacccgcg taggcgcggc
    43081 agatgacctg gcttccgggc gctcaacctt tatggtggag atgactgaaa ccgccaatat
    43141 tttacataac gccaccgaat acagtctggt gttaatggat gagatcgggc gtggaacgtc
    43201 cacctacgat ggtctgtcgc tggcgtgggc gtgcgcagaa aatctggcga ataagattaa
    43261 ggcattgacg ctgtttgcca cccactattt cgagctgacc cagctaccgg agaaaatgga
    43321 aggcgtcgcc aacgtgcatc tcgatgcgct ggagcacgga gacaccattg cctttatgca
    43381 tagcgtgcag gatggcgcgg cgagcaaaag ctacggcctg gcggttgcag ctctggcagg
    43441 cgtgccaaaa gaggtgatta agcgcgcacg gcaaaaactg cgtgagctgg aaagcatttc
    43501 gccgaacgcc gccgctacgc aagtggatgg tacgcaaatg tctttgctgt cagtgccaga
    43561 agaaacttcg cctgcggtcg aagcactgga aaatctcgac ccggattcac tgactccgcg
    43621 tcaggcgctg gagtggattt atcgcttgaa gagtctggtg taataacaat tcccgatagt
    43681 cttttgctat cgggaatatt aacgacaact gacgaatcaa ataaaaatac cctgtataat
    43741 aggaaagctt attttacagg gtaaaaccat gccatctaca cgctatcaaa aaatcaatac
    43801 ccatcactat cgccatatat gggtcgttgg tgatattcat ggtgaatatc agttattaca
    43861 atcccgctta catcaactct cttttttccc cgaaaccgac ttacttattt ctgtcggcga
    43921 taatattgat cgtgggccgg agagtcttga cgtcctgcgc ctgctaaacc agccctggtt
    43981 tatctccgtt aaaggcaacc acgaagcaat ggcgctggat gcgttcgcga ccggcgatgg
    44041 caatatgtgg cttgccagcg gtggtgactg gtttttcgat ttaaatgatt cagagcaaaa
    44101 agaagctaca gatctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
    44161 cgacaacata aaatatgtca tcgcacatgc agattatccg gggagtgaat atctctttgg
    44221 taaagaaata gcggagagcg aattactctg gcctgttgat cgtgtgcaga aatcgcttaa
    44281 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
    44341 taacattcag acgttcgcta accagattta tattgatacc ggcacgccga aaagcgggcg
    44401 gctgtcattt tataaaataa agtagtctca tgcttcttct gtgaagcatg agtaaccaaa
    44461 tgttattgca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
    44521 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
    44581 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
    44641 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
    44701 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgcg
    44761 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
    44821 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
    44881 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
    44941 tcccgcatcc agatagatac aacttcccgg ctgaacaaga cttgccgcca gcttgccaat
    45001 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
    45061 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
    45121 ttgtaaatcc cgacgaatgg tcatatgtga cacattcatt ctgtccgtta gttcagcaat
    45181 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
    45241 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
    45301 gcggcgcaac taacgcttag ttcacataaa ataacacaca atgttaattt atgtgaatca
    45361 gctcaccata tcgttatctt ccagcgctta tattcacaat atcaaacaaa atatcactta
    45421 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
    45481 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtctttc tacctggggc
    45541 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
    45601 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
    45661 gcccaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
    45721 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
    45781 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
    45841 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggca
    45901 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
    45961 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
    46021 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
    46081 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
    46141 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
    46201 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
    46261 gccagtaacg caggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
    46321 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
    46381 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
    46441 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
    46501 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
    46561 agcaaggttg caaacagatc tatttcaaat actgctctac tttcgacagt acggcgaaag
    46621 gtaatatcgg cccggttacc gatgcattaa tggatgctct cgacacgccg tttacggtct
    46681 tctctccggc cctgccggtc aacggacgta cggtttatca ggggtatttg ttcgtaatga
    46741 atcaactgct ggccgaatcc gggatgcgcc atcatccggt aaatcccatg accgacagct
    46801 atcttccccg tctggttgaa gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg
    46861 ttttcgaaca aggtgtggat gccgttcgtc aagagctggc tcgcttacag caagagggct
    46921 accgctacgc ggtgcttgat gcgctgaccg aacaccatct ggaaattcag ggagaagcct
    46981 tgcgcgatgc cccactggta acgggcggtt ctggtctggc gattggcctg gcccggcagt
    47041 gggcgcaaga aaacggtaac caggctcgcg aagcagggca tccgctcgct gggcgcggcg
    47101 tagtgctctc cggttcatgc tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa
    47161 ttgcaccagc ccgtgaagtt gatgtggcac gctgcctctc aaccgaaact ctggccgctt
    47221 atgcacacga actggcagag tgggttctgg gccaggaaag tgtacttgct ccactggttt
    47281 ttgccaccgc cagcactgac gcattggcag caattcaaca gcaatacggt gcacaaaaag
    47341 ccagtcaggc agtagaaact ctattttctc aactagcggc gcggttagca gcggaaggcg
    47401 tgacacgctt tattgtcgca ggcggtgaga cctccggcgt agtcacacag agcctgggca
    47461 taaaagggtt tcatattggc ccaaccattt cccccggcgt gccgtgggta aacgcactgg
    47521 ataagcctgt ctcactcgcc cttaaatctg gcaacttcgg tgatgaagcc tttttttcac
    47581 gagcccaaag agagttttta tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga
    47641 ggagatgacg cggattgcca gttcattctt tcagcgcggc tatgcaaccg gttcggctgg
    47701 caatctgtcg ctgcttttac ctgacgggaa tttactggcg acaccgacag gttcatgcct
    47761 gggcaatctc gatccgcagc ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg
    47821 tgacaaaccc tcgaaagagg tgctctttca tctggcgctg tatcgcaaca atccgcgctg
    47881 taaagcggtg gtgcatttgc acagcacatg gtcgacggcg ctttcctgcc tgcaagggct
    47941 ggacagcagc aacgttattc gtccgttcac accatacgtg gtgatgcgga tgggaaatgt
    48001 cccgctggtg ccttattacc gaccgggcga taaacgcatc gcacaggatc tggcggaact
    48061 ggcagcagac aatcaggctt ttttactggc aaatcatggc ccagtggttt gcggtgaaag
    48121 cctgcaagaa gccgccaaca atatggaaga gctggaggaa acggcaaagc tgatttttat
    48181 tctcggtgac cgcccgatcc gttatctgac cgcaggtgaa attgcggaat taaggagtta
    48241 atgcaatgcc tcgttttgca gctaatttat ccatgatgtt caccgaagtg ccttttattg
    48301 aacgcttcgc cgcagcgcga aaagccgggt tcgatgctgt ggaatttctg tttccctatg
    48361 actactccac cctgcaaatc caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt
    48421 tcaataccgc tcccggagat attaatgcgg gggaatgggg attatccgcc cttcccggac
    48481 gtgagcacga agcacacgca gacatcgacc tggcactgga atatgcattg gcgctcaatt
    48541 gtgaacaagt ccatgtgatg gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc
    48601 gggcagtatt tatcgataat ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc
    48661 gaatattagt tgaagcactc agccccgacg ttaagcctca ttatctcttc tccagccagt
    48721 atcaggcact ggctattgtt gaagaggttg cgcgagataa cgtgtttatt caactagata
    48781 cttttcatgc gcaaaaagta gatggcaacc tgacgcattt aatccgcgac tacgctggga
    48841 aatatgcgca cgtacaaatt gccggactac ctgatcgaca tgaaccggac gatggagaaa
    48901 tcaactatcc gtggctgttc cgcctgttcg atgaggtggg atatcagggc tggatcggtt
    48961 gtgaatataa acctcgtggc ctcaccgaag aagggcttgg ctggtttgac gcctggcgct
    49021 aattcgttaa atccctaatt acaacgtacc catacatccc cccatctacg gatggggtag
    49081 gttaactata actttcagac agggtttccc atgtccacaa ttacattgtt atgcattgcg
    49141 ttagctggcg taatcatgct gttgctgctg gtcatcaagg caaaggtaca accattcgtt
    49201 gctctgctcc tcgtcagcct gttagtcgca cttgcggcgg gtataccggc gggcgaagtg
    49261 ggtaaagtga tgatcgccgg gatgggcggt gtgcttggtt ccgtcactat tattattggt
    49321 ctgggcgcta tgctggggcg tatgatcgaa cactctggtg gtgcagagtc actggctaat
    49381 tatttcagtc gcaagttagg tgacaaacga actatcgctg cgctgactct ggcagcgttc
    49441 ttcctcggta ttcccgtctt ctttgatgtc ggctttatta ttcttgcgcc aatcatttac
    49501 ggttttgcca aggttgccaa aatatcgcca ctcaaatttg gcctgcctgt cgctggaatc
    49561 atgctaactg ttcatgtggc ggtaccgccg catccaggcc ctgtcgccgc agcggggtta
    49621 ctccacgcag acatcggctg gctaaccatc atcggtattg cgatttctat tcccgtaggg
    49681 attgttggct actttgcagc gaaaataatc aatacgcgcc aatatgcgat gtcggtggaa
    49741 gtactggaac agatgcaact ggctccggcc agtgaggaag gcgcaacaaa attaagcgat
    49801 aaaataaatc caccgggcgt cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt
    49861 atcatggcgg gtacggtttc cgcaacactg atgccgcctt cgcatcccct gcttggtacg
    49921 ctacagctga tcggctcacc aatggtagct ctaatgattg cgctggtgct ggcattctgg
    49981 ttattggctt tacgtcgcgg ctggagctta caacacacca gcgacattat gggctcagcc
    50041 cttcccactg cggcggtagt gattttggtt actggtgctg gaggggtatt tggcaaagtg
    50101 ctggtggaat cgggcgtcgg caaagccctt gccaatatgc tacaaatgat tgacctgcct
    50161 ctgttaccag ccgcatttat tatttcactg gctctgcgtg catcgcaggg gtcagcaacc
    50221 gtagcaatcc tgacaaccgg cgggttactc tcagaagcgg tgatgggatt gaatccgatt
    50281 cagtgcgtat tggtgacgct ggcagcctgc tttggtgggc ttggcgcgtc acatattaat
    50341 gactcagggt tctggattgt gaccaaatat ctggggttgt cggtagcaga cggtctgaaa
    50401 acctggacag tgttaacgac cattctcggt tttaccggat tcttaattac ctggtgcgta
    50461 tgggcggtaa tttgacctta ataaaaaggt ccgatgggca tcggaccttt tatttaacgc
    50521 ttaccttcca ccagcagcgg cgggatgctc ggcaccattg gcgcgtcatc aatatctttc
    50581 tgcgtcatgc ggaacgcttc gggataatgt tcgcggctgg tacggcgcag cgattcggta
    50641 tcgcgccagg tataaaggca atgctggcac tgatataccg tccagacatc tttcaccggc
    50701 gatttcgcca tcacttcaat ctgttcatcg gcacaacgtg gacaaatcat cttcttctcc
    50761 ttatttacgt gcagccagca tagcggtcag tttttcagcc caggctttgg tttccggtaa
    50821 atccaccacc ggttggctgt agtgaccacg gttgtccggg gcgacaggcg tagtggcgtc
    50881 gataatcagc ttgtcggtga tccccgcagg gcttgagcct ggatcgagtt ccagcacgga
    50941 catattcggc aactgcacca aatcccctgc cgggttcact ttcgaggaga gcgcccacat
    51001 cacctgcggc aggttgaacg ggtcaacgtc ttcatcgacc ataatcacca tcttcacgta
    51061 gcccagacca tgcggcgtgg tcattgcgcg caggcccacc gcgcgggcaa agccgccgta
    51121 gcgttttttg gtggagataa tcgccagcag gccatgggtg tacatggcgt ttaccgcctg
    51181 cacttcaggg aactcggctt tcagctgctg atacagcggc acgcaggtgg ctggccccat
    51241 caggtagtcg atttcggtcc acggcatacc gaggtacagc gattcgaaaa tcggcctggt
    51301 gcggtaagag actttatcga tgcgcaccac ggtcatgtta cgcccgccgg agtagtgccc
    51361 ggtaaactca ccgaacggcc cttcgatttc gcgtttacgg ctttcgatga ccccttcgag
    51421 gatcacttct gaaccccacg gcacatcaaa accggtcaac ggggcggtgg cgatcgggta
    51481 cgggctttca cgcagcgcgc ctgccatttc gtactcggac tgatcatatt tcagcggcgt
    51541 ggcccccatc agcgtgatga tcggatcgtt accgagcgtg atcgcaatcg gcagatcttc
    51601 accgcgctct tctgctttat gcagatgcag ggcgatatcg tgcatcggca ccggttgcag
    51661 gccgagctta cgcttgccct tcacttccat gcggtagatg ccgacgttct gcttgccgaa
    51721 gttatccggg tcgagcggat cgcgggaaac cacgcacgct ttgtcgagat agaaaccgcc
    51781 atcgccatcg tttaaacgaa acagcggcag gatatcgaac aggttgatct cgtcgccatc
    51841 aacggtgttc tgcgcccagg ctggattggc gcggcgctcc ggggcaatcg ggaagttatc
    51901 ccagcggcgg ataaactcat caatctgctt tttaaccggg gcatttggcg gcaggccgag
    51961 ggaaatcgcg tggttctgcc aggaaccgat ggtgttcatc gccacgcggg catcggtaaa
    52021 gccgcgaata ttatcaaacc acagcgcggg cgcgccgtcg ccgatacgcc cggtggcgtt
    52081 agctgctgct gccagatccg gctcggcgtt cacttcttcg ctgattttca gtaactggcc
    52141 gtggtcatca agcgcctgta aaaagctgcg taaatcatca aatgccatta ttcattctcc
    52201 tgagaaaaat tccgggcctg cggcaatcct tgccagcgcc tggcgtgggg atgttcgagg
    52261 ccaaattgat ccagcacgcg ggctaccaca tggtggacaa tgtcatctac cgtttcggga
    52321 tggttataaa aggcaggcat cggcggcacc atcgccacgc ccatgcgtga aagtgcgagc
    52381 atattttcga gatggatggt gctaagcggc atttcacgcg gcaccagcac cagtttgcgg
    52441 ccttctttga gcacgacgtc cgccgcgcgc cctaccaggc catcggcgta accagcgcgg
    52501 ataccggcga gcgttttcat actgcacgga ataacgatca tgccgtcggt acgaaaagaa
    52561 ccggatgaga tgatcgccgc ctgatccgcc gggttatggc tgaagtctgc gagggcagca
    52621 acatcgcgag cgctgtaagg cgtttccagt tcaatggtgg ttttcgccca cttcgacatc
    52681 accagatgag tctcgacatt cggcatctcc cgcagcgctt gcagtaatgc cacaccaaga
    52741 ggcgcaccgg tagcccctgt catcccgacg atcagtttca ttgcttatcc tcttaattgt
    52801 tcgtatacga acgttattat taacatactc cttcatttcc acagcggcaa ggattgaaaa
    52861 gtaacaacat cttcttcgtc actaaaaaac cgctatgata gcgacagaga attagcccgg
    52921 agtttacatg gcgttacgaa ataaagcgtt ccatcagtta cgacagcttt ttcagcagca
    52981 cacggctcgc tggcagcacg agttacctga cctgaccaaa ccacagtacg cggtgatgcg
    53041 cgccattgct gataaacctg gtatcgaaca ggtggcactg atagaagcag ctgtcagcac
    53101 caaagcaacg ttagcagaaa tgttggcaag aatggaaaat cgtggcttag tcagacgtga
    53161 acatgacccc gctgataagc ggcgacgctt tgtctggttg actgccgaag gcgaaaagat
    53221 acttgctgcg gcgataccca ttggtgacag cgtggatgag gaatttttgg ggcgtttgag
    53281 tggagaagag caggagctgt ttatgcagct ggtgcgcaag atgatgaacg catagggggc
    53341 aaatttccgg atgcgacgct atcgcgtctt atccagacta caaatacacc gactcaccaa
    53401 ccgaaagaaa aaaggccagc ctcgcttgag actggccttt ctgacagatg cttacttact
    53461 cgcggaacag cgcttcgata ttcagcccct gcgtttgcag gatttcgcgc aaacggcgca
    53521 ggccttcaac ctgaatctgg cgaacacgtt cacgggtgag gccaatttca cgacctacat
    53581 cttccagtgt tgccgcttcg taccccagca aaccgaatcg acgtgccagc acttcacgct
    53641 gtttggcgtt cagctcgaac agccatttga cgatgctctg cttcatatcg tcatcttgcg
    53701 tggtatcttc cggaccgttc tctttttcat cggccaggat gtccagcaac gctttttcgg
    53761 aatcaccacc cagcggggtg tctaccgagg taatgcgctc gttaagacga agcatacggc
    53821 tgacgtcatc aactggctta tccagttgct ctgcgatctc ttccgcactt ggttcatggt
    53881 ccagcttatg ggacaactca cgtgcggttc gcaggtaaac gttcagctcc tttacgatgt
    53941 gaatcggcaa acgaatagta cgggtttggt tcataatcgc ccgttcaatc gtctggcgaa
    54001 tccaccaggt tgcgtatgtt gagaagcgga aaccacgttc cgggtcaaac ttctctaccg
    54061 cgcggatcag ccccaggttg ccctcttcga taaggtccag caacgccaga ccacgattgc
    54121 cataacggcg ggcaattttt accaccagac gcaagttact ctcgatcatc cggcggcgag
    54181 aggcgacatc tccacgcagt gcgcgacgcg caaaataaac ttcttcttcg gccgttaaca
    54241 gtggtgaata accaatctca ccaaggtaaa gctgagtcgc gtccaacaca cgctgtgtgg
    54301 ctccctgcga taacagttcc tcttcggcca aatcgttatc actgggttcc tcttctacta
    54361 aggccttttc gtcaaaaacc tcaactccgt tctcatcaaa ttccgcatct tcatttaaat
    54421 catgaacttt cagcgtattc tgactcataa ggtggctcct acccgtgatc ccttgacgga
    54481 acattcaagc aaaagcctgg ttccgccgat ttatcgctgc ggcaaataac gcagcgggtt
    54541 tacggatttc cccttgtaac gaatttcaaa atgcaagcgt gttgaactgg ttccggtgct
    54601 acccatggtt gctatttttt gccccgcctt cacttcttgt tgttcccgga ccagcattgt
    54661 gtcgttatgg gcgtaggcac tcaggtaatc atcattatgt ttgatgataa tcagattacc
    54721 gtagccgcgc agcgcgttac cggcataaac aacgcggcca tctgcggtcg cgataattgc
    54781 ctgtcctttg ctgcctgcga tatcaatccc cttgttgccc ccctcagaag cgccaaaggt
    54841 ttcgatcact ttgccctcag tcggccagcg ccaggtggag ataggcgtac tggttgatgt
    54901 actgctgaca gttggctcgg ttgtgcttgc tgttggtacc gttacaggcg ctgtgaccgt
    54961 ggtcgcagct ggcttgttgt tcggcaacat tttgttagca ctctgttcac ccgaagattc
    55021 agaatacgta attgtcggtt gcgacgcaac agcaacggtg gaattttgtg caggcttgat
    55081 cacaactcct tgctctgctg cgtcggcctg ggtaatggca tttccgccag tgattggcgt
    55141 accggaagca ttacccacct gcaaggtctg accaacgttc agcgcgtatg gtgcctgaat
    55201 attgttgcgc tgagcaaggt cacggaaatc gttgccagta atccaggcga tatagaaaag
    55261 tgtgtcgcct tttttcacgg tataggtact gccgctataa ctgcctttcg gaatgttccc
    55321 atactgacgg ttatagacga tgcgtccgtt ttccatctgt accggctgct gagctactgg
    55381 ctgcactggc tggatttgcg gttgttgagt agcctgaatt tgtggctgct gtaccggctg
    55441 aatttgcggt tgctgggctg tagacgtcgt ccccattttc ggcggcggcg taatcaacat
    55501 accagaattg gtatttgcag gcgcattgcc attaacggag ctgaccggtg ccggtggatt
    55561 tgaagtgtca gaacagcctg ccagccatag cgaaaccagt gacaaagccg caatgcggcg
    55621 aacggtgaat tttgggcttc ccgcgctcat ttatccccca ggaaaaattg gttaataacc
    55681 agtgacataa ttaccgtgca aggcacccta ctgaacactg gaaaagatgt tcacgatacg
    55741 ctgacctgcg gcaaaataac caggaaaaat ccaggtattt cctcacgttt taagccagct
    55801 cacccttcac taaaggtaca aagcgcacgg cctccacggt atcgataata aattcgcctc
    55861 cccgacgacg cacccgtttc aaatactggt gctcctcccc tacgggtaag acgagaatcc
    55921 cgccttcgtc cagctgcgtc attagcgcag ttggaatttc cggcggtgcc gccgtaacaa
    55981 tgatagcgtc aaacggcgcg cgtgcctgcc aaccttgcca tccatcgcca tgacgggttg
    56041 aaacattatg taaatcaaga tttttcaggc ggcgacgcgc ctgccactgc aaacctttaa
    56101 tccgttcgac cgagcaaaca tgctggacaa gatgcgccag gattgccgtt tgatatcccg
    56161 aaccagtgcc aatttccagc acccgcgact gcggcgtcag ctcgagtaat tcggtcattc
    56221 gcgccaccat atacggctgc gaaattgtct gcccctgacc tatcggcaag gcgatattgt
    56281 cccaggcttt ttgttcaaac gcttcatcaa cgaatttttc acgcggcacg gcggcaagtg
    56341 cattcagcac ctgctcatcc tgaatacctt gcgcacgtaa ttgatccaga agtgcttgta
    56401 cgcgtctgct taccattgcg tgccaactcc cacgctgttt aaccagtctg aaaccacatc
    56461 ttgcgcgcta tgcgcagtta aatccacatg cagcggcgtg atggagacat agccctcatc
    56521 taccgcagca aaatcggtcc ccggaccggc atcacattta ccgcccggcg ggccaatcca
    56581 gtacagcgta ttgccgcgcg gatcttgctg cgggatcacc tgatctgccg gatgtcgtgt
    56641 accgcagcgc gtcacgcgaa tacctttgat ttgatccaag ggtaaatccg gaacgttaat
    56701 attaagaata cgcccggtgc gcaacggctc tttacacagt gcgcgcaaaa ttgaacaggt
    56761 taccgccgcg gcagtgtcgt aatgtttatg cccgtcaagc gagacggcaa gcgccggaaa
    56821 acctaaatga cggccttcca tcgcggcggc taccgtaccg gaataaataa catcatcccc
    56881 cagattcggc ccggcgttaa ttccggacac aacaatgtcc gggcgcggac gcatcagagc
    56941 attcacgcca agatagacgc aatcggtcgg ggttcccatt tgcacagcaa tatcaccatt
    57001 ttcaaaggta aacgtgcgca gggaggattc cagtgtcaga gaatttgaag cgccgctgcg
    57061 gttacgatcg ggggcgacca cctgaacgtc agcaaactca cgcaaggctt tcgccagcgt
    57121 ttgtataccg ggtgcatgta ccccgtcatc attactcagc aatatgcgca taatcacctg
    57181 ttgtgttgat aagttccctg acaacgctgg ttgcaaaact acccgccgga agccagaaac
    57241 ggatctctac ggtgacgtca tcccaccaat tccagcttaa ttgttgcgga tacagcagca
    57301 tcgctctgcg cgcggcttca actttttcgc gcaccagtaa agcttgtaat tcagtttctg
    57361 cggcgacagc tgcttgttcg aatgccagcg cttcacgctg agttccccat tcgccactgc
    57421 ccggcaatgc ggcggttatc atcagctctt tatcgttaac gcgacgctgt aattccgcca
    57481 gttcttcggt ggttgcgaca aaccagctac cacgtccggc taattgtagc gcatcgccgt
    57541 caacaacttg attaacgtct gcttttttga ggcgctcagc aacaatctga ttaaacaacg
    57601 cactgcgggc tgccgacaac caaaaactcc gtttattgcg atcgcgcacc ggagtattgg
    57661 tttgcgccca gcgctgcgcc ccctgcaagt tgctaccgcc aatcccaaaa cgttgggcac
    57721 cgaagtagtt cggtacacct tttacgcaaa tatcgttcag acgttgttca acgtcatcgc
    57781 gattgctcac ttcgcgcaga accagggtaa aggcgtttcc tttcagcgcc cctaaacgca
    57841 gcttgcgctt gtgccgcgca tactccagca cctggcagcc ttccagttga aaggcgctca
    57901 gatcgggcat ttccttgccc ggcacgcgag cgcataacca ctgttccgta acagcatgtt
    57961 tgtctttttg cccagcgaag ctgacttcac gggcatgaat tttcaggaat ttcgccagtg
    58021 catccgccac aaaacgggta ttgcagccgt ttttgaggat tctaaccaga atatgctcac
    58081 cttcaccatc aggctcaaag cccaaatctt ccaccaccac aaagtcttcc ggattggctt
    58141 tcagcagccc ggtgccttgc ggtttaccgt ggaggtaagt gagattatca aactcaatca
    58201 ttttgttgcc ttaatgagta gcgccaccgc ttcacaggca atcccttccc cacgtccggt
    58261 aaatcccagt ttttccgtag tagtggcttt cacgttaaca tcatccatat ggcagccgag
    58321 atcttcggca ataaacacgc gcatttgtgg aatgtgcggc aacatcttcg gtgcctgagc
    58381 gatgatagtg acatcgacgt tgccaagggt ataacccttc gcctgaatac gacgccaggc
    58441 ttcgcgtagc agctcgcggc tatcggcacc tttaaatgcc ggatcggtat ccgggaacag
    58501 cttgccgata tcccccagcg ccgccgcgcc aagcaatgca tcggtcaacg catggagcgc
    58561 cacgtcgcca tcagaatgcg ccagcaatcc tttttcgtaa ggaatgcgta cgccaccaat
    58621 gataattggg ccttcaccgc caaaggcatg tacgtcaaaa ccgtgtccaa ttcgcattat
    58681 gtattctcct gatggatggt tcgggtgagg taaaactcgg ccagtgccaa atcttccggg
    58741 cgcgtgactt taatgttatc cgcacggcct tcgaccaact gaggatggaa tccgcaatat
    58801 tccagcgccg aggcttcgtc ggtaatagtc gcgccttcat ttagagcgcg cgtcagacag
    58861 tcatgtaaca gctcacgagg gaaaaattgc ggcgtcagcg cgtgccataa gccgttgcga
    58921 tcaacggtat gagcaatggc atttttgccc ggttcggcac gtttcatagt atcgcgcact
    58981 ggtgcggcga ggatcccccc cgtgcggctg gtttcgctca acgccaacaa tcgcgcgagg
    59041 tcatcctgat gcaaacaagg acgagcggcg tcatgcacca atacccactg cgcgtcgcca
    59101 gcggctttca gacctgccag cacggaatcg gcacgctcat caccgccatc tacaacggtg
    59161 atttgcggat gattcgccag aggaagttgt gcaaaacggc tatcgccagg acttatggca
    59221 atgacgactc gtttcacccg gggatgcgcc agcagcgcat gcaccgagtg ttcaagaatg
    59281 gtttgattac cgattgagag atattgctta ggacattccg tttgcattcg acggccaaat
    59341 ccggccgccg gaaccacggc gcaaacatcc aaatgagtgg ttgccatgtt aattcccggg
    59401 ctgatttatc gattgttttg ccccgcagac tgtgcgcgct tcgacgcgtc aggcaccaga
    59461 cgataaaaag tttcgcccgg cctggtcatg ctgagttcat tacgcgcacg ctcttcgagc
    59521 gcctcctggc cgccattgag atcgtcaatt tcggcaaaaa gttgatcgtt tcgcgcttta
    59581 agtttcgcgt ttgtagcttg ctgtgccgcc acatcatcat tgacgcgggt atagtcatgt
    59641 ataccgttct taccgaacca cagcgaatac tgtagccaga ccagaatagc cagcaacagc
    59701 agcgttagtt tacccatcct gccccctgaa aaacggcatc atcatcccat gcatccgaag
    59761 acgactctac atcctctgtt ggggataccg cgacaacgcg ggcaaatgta ccacatttgt
    59821 ccattgttac gtatacccag ggcgtgcaga acataatctc attattagtt acggtttgaa
    59881 ttatgaacag aggagacggg aaagtacaaa ttagcccagt agccacataa acagtgcgcc
    59941 aaacataatg cctactgtca ccagggtgaa aacaatactg tagcgtagct ttccgtccat
    60001 caatgaatgc agcgcaatcc ccaccactac cgcgacgggc atcagcgcca gaaagaaagg
    60061 ccaggtgtag ataaagaaga acagcgtgtt agagccataa atcaacatcg gcatcgccag
    60121 cgcaaataac caggagataa aaccgaccac ggcaccaggc agtgaccatg tggtttcttc
    60181 atcctcagta aggctgtcgt tatttgttag tgtaatgtta tggctattac gcatatttga
    60241 tcctgttact ttgacgaacc gggcatggaa acccggtggt gtctcaggat ctgataatat
    60301 cgttctgtct caacagatct aataattgct gtaccaaatt tgttactaat tgttcaccat
    60361 tgagatgaat ttctgccgat tcaggcgctt cgtaaacgga atctattccc gtaaagttgc
    60421 gcagttcacc ggcacgcgct ttcttatata agccttttgg atcgcgggct tcgcaaatcg
    60481 ccagcggcgt atcgacaaac acttcgataa agcgcccttc tcctacgcgt tcgcgaacca
    60541 tctggcgttc ggcgcggtgt ggcgagataa atgcggtcag cactaccagt ccggcttcaa
    60601 ccatcaaatt cgccacttca ccgacgcgac ggatattctc tttacgatcg gcatcgctaa
    60661 aaccgagatc gctgcataat ccgtggcgaa cattatcgcc atccagcaga tacgtactga
    60721 cgccgagttt atgtaacgcc tcctccagcg ccccggcgac cgttgattta ccggacccgg
    60781 agaggccggt aaaccacagc actacaccac gatgaccgtg gtgtagctcg cgttgttgca
    60841 cagtgaccgg atggctatgc cagacgacgt tttcgtcatg cagcgccatt atttatcccc
    60901 cagcaaatcg cgcgcgcccc agtgcggaaa gtggcgacga accagagcat tcaattccag
    60961 ttcgaatgca ctgaattcag atggcgcagc agttgcctgg ctaactggct cgtgcaccat
    61021 accggcaccc acggtcacat tgctcaggcg atcgataaaa atcagcccac ccgtcaccgg
    61081 attttgttga taacgatcta acaccagcgg ctcgtcaaaa gtgagatcca cgaggccgat
    61141 cccattcagt ggcaggtttt caacttcacg ctgggtaagg ttattaatat caacctgata
    61201 gcgaatgcca tcaacacgcg cgcgcgtctt cttaccggca attttgatgt cgtaactctg
    61261 ccctggagaa agcggctgtt ccgccatcca taccacatcc accgacgcgc tctgcaccgc
    61321 cggtaacgct tcgtctgccg ccagcagcag atcgccacgg ctgatgtcga tctcatccgt
    61381 cagcaccagg gtgatcgctt ctccggcaaa ggcttcttcg cgatcaccat caaaagtcac
    61441 gatccgcgcg acgtttgatt ccacaccaga gggcagcact ttgacacgtt gcccgacttc
    61501 cacgcgaccg gatgccagcg ttccggcgta accacgaaaa tcgagattcg ggcgattaac
    61561 gtactgcacc gggaagcgca ttggctgagc atccaccact cgctggatct ccacggtttc
    61621 cagcacttcg agcagtgtcg gaccgctgta ccacggcata ctttcacttt gcgatgccac
    61681 gttgtcgcct tccagtgcag agagcggcac aaagcggata tccagattac ccggcagctg
    61741 cccggcaaag gtcaaataat cttcacgaat acgggtgaac gtctcttcac tgtaatccac
    61801 cagatccatt ttgttgatcg ccacgaccag atgtttgctc cccaacagtg tggagataaa
    61861 actgtgacga cgggtttgat cgagcacgcc tttacgggca tcgatcagta agatcgccag
    61921 ttcacatgtc gatgcgccag tcgccatatt gcgggtgtac tgctcgtgcc ctggggtgtc
    61981 ggcgataata aatttacgct tctcggtaga gaaatagcgg taggccacgt caatggtgat
    62041 gccctgttcg cgctcagctt gcaggccgtc caccagcaga gccagatcca gcttttcgcc
    62101 ctgggtgccg tgacgcttac tgtcgttatg cagcgatgag agctgatctt cgtagatttg
    62161 gcgggtatcg tgcagcagac gaccaatcag agtacttttg ccgtcatcga cgctaccaca
    62221 ggtcagaaaa cgcagcaggc ttttatgttg ttgcgcaatc atccaggctt cgacgccgcc
    62281 ttcattggcg atttgttgtg caagtgcggt gttcatctta aaaatacccc tgacgttttt
    62341 tcagctccat agaccccgcc tggtcgcggt caatcacgcg gccctgacgt tcactggtgg
    62401 tggaaaccag catctcttcg atgatttccg gcagtgtttg tgcatttgac tccaccgcac
    62461 cggtcagcgg ccagcagccc agcgtacgga aacgcaccat ccgtttttta atcacttcgc
    62521 ccggttgcag gtcgatacgg ttgtcatcaa tcatcatcaa cataccgtcg cgttccagaa
    62581 ccggacgttc cgcagcgaga tatagcggaa caatgtcgat attttccagc cagatgtatt
    62641 gccagatatc ctgctcggtc cagttagaga gcgggaagac gcggatgctt tcgcctttgt
    62701 taatttgccc gttgtagttg tgccacagct ccgggcgctg atttttcgga tcccagcgat
    62761 ggaagcggtc acggaaagag taaattcgct ctttagcgcg ggatttctct tcgtcacggc
    62821 gcgcaccacc gaaggcggca tcaaaaccgt atttgttcag cgcctgtttc aggccttcag
    62881 ttttcataat atcggtatgt ttcgcgctgc cgtgcacgaa tggattaatc cccatcgcca
    62941 cgccttccgg gtttttatgc accagcagtt cgcagccgta ggctttagca gtacgatcgc
    63001 ggaactcata catctcgcgg aatttccagc cggtatcgac atgcagcaac gggaaaggca
    63061 gcgtacctgg ataaaacgcc ttgcgcgcca gatgcagcat gacgctggaa tctttaccga
    63121 tagagtagag catcaccgga tttgagaatt ctgccgccac ctcgcgaata atgtggatgc
    63181 tttccgcctc cagttgccgc aggtgagtaa gtcgtatttg atccataacc gttcctttgc
    63241 aataccgcta ttttcttgcc atcagatgtt tcgactatag ggagcgtaag agaacgaatg
    63301 aaattaccaa ttagaatgag tagttcctta acggaataac gatttggcaa agctaatatc
    63361 aaaaagtgct taaggcaccg gatttcgggc gtttaggaag atttgaaatt gttttagcgc
    63421 agcggcagtt tcatactatg gcggtaaaaa aatttgcatg gtatttaagg actcactatg
    63481 ttttccgcat tgcgccaccg taccgctgcc ctggcgctcg gcgtatgctt tattctcccc
    63541 gtacacgcct cgtcacctaa acctggcgat tttgctaata ctcaggcacg acatattgct
    63601 actttctttc cgggacgcat gaccggaact cctgcagaaa tgttatctgc cgattatatt
    63661 cgccaacagt ttcagcaaat gggttatcgc agtgatattc ggacatttaa tagtcggtat
    63721 atttataccg cccgcgataa tcgcaagagc tggcataacg tgacgggaag tacggtgatt
    63781 gccgctcatg aaggcaaagc gccgcagcag atcatcatta tggcgcatct ggatacttac
    63841 gccccgctga gcgatgctga cgccgatgcc aatctcggcg ggcttacgtt acaaggaatg
    63901 gatgataacg ccgcaggttt aggtgtcatg ctggaattgg cagaacgcct gaaaaatacg
    63961 cctaccgagt atggtattcg atttgtggcg accagcggcg aagaggaagg gaaattaggc
    64021 gcagagaatt tactcaagcg gatgagtgac accgaaaaga aaaatacgct gctggtgatt
    64081 aatctcgata acttaattgt tggcgataaa ttgtatttca acagcggtgt aaaaacccct
    64141 gaagcagtaa ggaaattaac gcgcgacagg gcgctggcaa ttgcgcgtag tcatggaatt
    64201 gccgcaacga ccaatccggg tttgaataaa aattatccga aaggcactgg gtgttgtaat
    64261 gacgcagaaa tattcgacaa agcgggcatt gctgtacttt cggtggaagc gactaactgg
    64321 aatcttggga ataaggatgg ttatcagcaa cgcgcaaaaa cacctgcctt cccggcggga
    64381 aatagctggc atgacgtaag actggataat caacaacata ttgataaggc acttcctgga
    64441 agaatagaac gtcgctgccg tgatgttatg cggataatgc taccgctggt gaaggagctg
    64501 gcgaaggcgt cttgatgggt ttgaaaatgg gagctgggag ttctaccgca ggggcgggga
    64561 attctaagtg atatccatca tcgcatccag tgcgcccggt ttatccccgc tgacgcgggg
    64621 aacacaaaac caaacttctc cataaattcc atagccgcgg tttatccccg ctggcgcggg
    64681 gaacactctt cgcgggtaat caatgatgat tcagtttccg gtttatcccc gctggcgcgg
    64741 ggaacacgaa cgcaatatca cggcgttctg cggcctcggc ggtttatccc cgctggcgcg
    64801 gggaacacca aaatattacg agcttcgtca ggccatggac cggtttatcc ccgctggcgc
    64861 ggggaacacg tcgggccggg taagtgaacc tgtcggtttt ccggtttatc cccgctggcg
    64921 cggggaacac actaagca
//
LOCUS       DAZFWL020000012        63814 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFWL020000012 DAZFWL020000000
VERSION     DAZFWL020000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 63814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.012"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            72..788
                     /locus_tag="ACHKNP_RS04235"
                     /old_locus_tag="pgaptmp_000847"
     CDS             72..788
                     /locus_tag="ACHKNP_RS04235"
                     /old_locus_tag="pgaptmp_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4703069.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            818..1318
                     /locus_tag="ACHKNP_RS04240"
                     /old_locus_tag="pgaptmp_000848"
     CDS             818..1318
                     /locus_tag="ACHKNP_RS04240"
                     /old_locus_tag="pgaptmp_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HGU4703070.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQITKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1410..4094
                     /locus_tag="ACHKNP_RS04245"
                     /old_locus_tag="pgaptmp_000849"
     CDS             1410..4094
                     /locus_tag="ACHKNP_RS04245"
                     /old_locus_tag="pgaptmp_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HGU4703071.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADISDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDGLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4091..5165
                     /locus_tag="ACHKNP_RS04250"
                     /old_locus_tag="pgaptmp_000850"
                     /pseudo
     CDS             4091..5165
                     /locus_tag="ACHKNP_RS04250"
                     /old_locus_tag="pgaptmp_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
     gene            complement(5253..6551)
                     /gene="exuT"
                     /locus_tag="ACHKNP_RS04255"
                     /old_locus_tag="pgaptmp_000851"
     CDS             complement(5253..6551)
                     /gene="exuT"
                     /locus_tag="ACHKNP_RS04255"
                     /old_locus_tag="pgaptmp_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HGU4703072.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7034..8446
                     /gene="uxaC"
                     /locus_tag="ACHKNP_RS04260"
                     /old_locus_tag="pgaptmp_000852"
     CDS             7034..8446
                     /gene="uxaC"
                     /locus_tag="ACHKNP_RS04260"
                     /old_locus_tag="pgaptmp_000852"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HGU4703073.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8461..9948
                     /gene="uxaA"
                     /locus_tag="ACHKNP_RS04265"
                     /old_locus_tag="pgaptmp_000853"
     CDS             8461..9948
                     /gene="uxaA"
                     /locus_tag="ACHKNP_RS04265"
                     /old_locus_tag="pgaptmp_000853"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HGU4703074.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10031..10582
                     /gene="ygjV"
                     /locus_tag="ACHKNP_RS04270"
                     /old_locus_tag="pgaptmp_000854"
     CDS             10031..10582
                     /gene="ygjV"
                     /locus_tag="ACHKNP_RS04270"
                     /old_locus_tag="pgaptmp_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HGU4703075.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10587..11831)
                     /gene="sstT"
                     /locus_tag="ACHKNP_RS04275"
                     /old_locus_tag="pgaptmp_000855"
     CDS             complement(10587..11831)
                     /gene="sstT"
                     /locus_tag="ACHKNP_RS04275"
                     /old_locus_tag="pgaptmp_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HGU4703076.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12230..13195)
                     /gene="alx"
                     /locus_tag="ACHKNP_RS04280"
                     /old_locus_tag="pgaptmp_000856"
     CDS             complement(12230..13195)
                     /gene="alx"
                     /locus_tag="ACHKNP_RS04280"
                     /old_locus_tag="pgaptmp_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HGU4703077.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13478..14464)
                     /gene="ygjR"
                     /locus_tag="ACHKNP_RS04285"
                     /old_locus_tag="pgaptmp_000857"
     CDS             complement(13478..14464)
                     /gene="ygjR"
                     /locus_tag="ACHKNP_RS04285"
                     /old_locus_tag="pgaptmp_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4703078.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14543..15222)
                     /gene="ygjQ"
                     /locus_tag="ACHKNP_RS04290"
                     /old_locus_tag="pgaptmp_000858"
                     /pseudo
     CDS             complement(14543..15222)
                     /gene="ygjQ"
                     /locus_tag="ACHKNP_RS04290"
                     /old_locus_tag="pgaptmp_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
     gene            complement(15299..15802)
                     /gene="ygjP"
                     /locus_tag="ACHKNP_RS04295"
                     /old_locus_tag="pgaptmp_000859"
     CDS             complement(15299..15802)
                     /gene="ygjP"
                     /locus_tag="ACHKNP_RS04295"
                     /old_locus_tag="pgaptmp_000859"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HGU4703079.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTPDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15887..17023
                     /gene="rlmG"
                     /locus_tag="ACHKNP_RS04300"
                     /old_locus_tag="pgaptmp_000860"
     CDS             15887..17023
                     /gene="rlmG"
                     /locus_tag="ACHKNP_RS04300"
                     /old_locus_tag="pgaptmp_000860"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HGU4703080.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSISDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17214..17297
                     /locus_tag="ACHKNP_RS04305"
                     /old_locus_tag="pgaptmp_000861"
                     /pseudo
     CDS             17214..17297
                     /locus_tag="ACHKNP_RS04305"
                     /old_locus_tag="pgaptmp_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17294..17710
                     /gene="higA"
                     /locus_tag="ACHKNP_RS04310"
                     /old_locus_tag="pgaptmp_000862"
     CDS             17294..17710
                     /gene="higA"
                     /locus_tag="ACHKNP_RS04310"
                     /old_locus_tag="pgaptmp_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HGU4703081.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17755..19773)
                     /gene="fadH"
                     /locus_tag="ACHKNP_RS04315"
                     /old_locus_tag="pgaptmp_000863"
     CDS             complement(17755..19773)
                     /gene="fadH"
                     /locus_tag="ACHKNP_RS04315"
                     /old_locus_tag="pgaptmp_000863"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HGU4703082.1"
                     /translation="MSYPLLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVASSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHHVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20099..22450)
                     /gene="ygjK"
                     /locus_tag="ACHKNP_RS04320"
                     /old_locus_tag="pgaptmp_000864"
                     /pseudo
     CDS             complement(20099..22450)
                     /gene="ygjK"
                     /locus_tag="ACHKNP_RS04320"
                     /old_locus_tag="pgaptmp_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(22467..23537)
                     /gene="ygjJ"
                     /locus_tag="ACHKNP_RS04325"
                     /old_locus_tag="pgaptmp_000865"
     CDS             complement(22467..23537)
                     /gene="ygjJ"
                     /locus_tag="ACHKNP_RS04325"
                     /old_locus_tag="pgaptmp_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HGU4703083.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(23671..25104)
                     /gene="ygjI"
                     /locus_tag="ACHKNP_RS04330"
                     /old_locus_tag="pgaptmp_000866"
     CDS             complement(23671..25104)
                     /gene="ygjI"
                     /locus_tag="ACHKNP_RS04330"
                     /old_locus_tag="pgaptmp_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4703084.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTVGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(25167..25616)
                     /gene="ebgC"
                     /locus_tag="ACHKNP_RS04335"
                     /old_locus_tag="pgaptmp_000867"
     CDS             complement(25167..25616)
                     /gene="ebgC"
                     /locus_tag="ACHKNP_RS04335"
                     /old_locus_tag="pgaptmp_000867"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HGU4703085.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25613..28705)
                     /gene="ebgA"
                     /locus_tag="ACHKNP_RS04340"
                     /old_locus_tag="pgaptmp_000868"
     CDS             complement(25613..28705)
                     /gene="ebgA"
                     /locus_tag="ACHKNP_RS04340"
                     /old_locus_tag="pgaptmp_000868"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HGU4703086.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQENEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(28889..29872)
                     /gene="ebgR"
                     /locus_tag="ACHKNP_RS04345"
                     /old_locus_tag="pgaptmp_000869"
     CDS             complement(28889..29872)
                     /gene="ebgR"
                     /locus_tag="ACHKNP_RS04345"
                     /old_locus_tag="pgaptmp_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HGU4703087.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30091..30423
                     /gene="ygjH"
                     /locus_tag="ACHKNP_RS04350"
                     /old_locus_tag="pgaptmp_000870"
     CDS             30091..30423
                     /gene="ygjH"
                     /locus_tag="ACHKNP_RS04350"
                     /old_locus_tag="pgaptmp_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HGU4703088.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(30465..31844)
                     /gene="ygjG"
                     /locus_tag="ACHKNP_RS04355"
                     /old_locus_tag="pgaptmp_000871"
     CDS             complement(30465..31844)
                     /gene="ygjG"
                     /locus_tag="ACHKNP_RS04355"
                     /old_locus_tag="pgaptmp_000871"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HGU4703089.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            32262..33782
                     /gene="aer"
                     /locus_tag="ACHKNP_RS04360"
                     /old_locus_tag="pgaptmp_000872"
     CDS             32262..33782
                     /gene="aer"
                     /locus_tag="ACHKNP_RS04360"
                     /old_locus_tag="pgaptmp_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HGU4703090.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(33936..34559)
                     /gene="nfeR"
                     /locus_tag="ACHKNP_RS04365"
                     /old_locus_tag="pgaptmp_000873"
                     /gene_synonym="yqjI"
     CDS             complement(33936..34559)
                     /gene="nfeR"
                     /locus_tag="ACHKNP_RS04365"
                     /old_locus_tag="pgaptmp_000873"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HGU4703091.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            34836..35600
                     /gene="nfeF"
                     /locus_tag="ACHKNP_RS04370"
                     /old_locus_tag="pgaptmp_000874"
                     /gene_synonym="yqjH"
     CDS             34836..35600
                     /gene="nfeF"
                     /locus_tag="ACHKNP_RS04370"
                     /old_locus_tag="pgaptmp_000874"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HGU4703092.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPLSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(35654..35729)
                     /locus_tag="ACHKNP_RS04375"
                     /old_locus_tag="pgaptmp_000875"
     tRNA            complement(35654..35729)
                     /locus_tag="ACHKNP_RS04375"
                     /old_locus_tag="pgaptmp_000875"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35694..35696),aa:Ile,seq:cat)
     gene            35854..36360
                     /gene="mug"
                     /locus_tag="ACHKNP_RS04380"
                     /old_locus_tag="pgaptmp_000876"
     CDS             35854..36360
                     /gene="mug"
                     /locus_tag="ACHKNP_RS04380"
                     /old_locus_tag="pgaptmp_000876"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HGU4703093.1"
                     /translation="MVEDILALGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(36439..38280)
                     /gene="rpoD"
                     /locus_tag="ACHKNP_RS04385"
                     /old_locus_tag="pgaptmp_000877"
     CDS             complement(36439..38280)
                     /gene="rpoD"
                     /locus_tag="ACHKNP_RS04385"
                     /old_locus_tag="pgaptmp_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGU4703094.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(38475..40220)
                     /gene="dnaG"
                     /locus_tag="ACHKNP_RS04390"
                     /old_locus_tag="pgaptmp_000878"
     CDS             complement(38475..40220)
                     /gene="dnaG"
                     /locus_tag="ACHKNP_RS04390"
                     /old_locus_tag="pgaptmp_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGU4703095.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNLELAKK"
     gene            complement(40331..40546)
                     /gene="rpsU"
                     /locus_tag="ACHKNP_RS04395"
                     /old_locus_tag="pgaptmp_000879"
     CDS             complement(40331..40546)
                     /gene="rpsU"
                     /locus_tag="ACHKNP_RS04395"
                     /old_locus_tag="pgaptmp_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGU4703096.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            40784..41797
                     /gene="tsaD"
                     /locus_tag="ACHKNP_RS04400"
                     /old_locus_tag="pgaptmp_000880"
     CDS             40784..41797
                     /gene="tsaD"
                     /locus_tag="ACHKNP_RS04400"
                     /old_locus_tag="pgaptmp_000880"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGU4703097.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(41840..43303)
                     /gene="ttdT"
                     /locus_tag="ACHKNP_RS04405"
                     /old_locus_tag="pgaptmp_000881"
     CDS             complement(41840..43303)
                     /gene="ttdT"
                     /locus_tag="ACHKNP_RS04405"
                     /old_locus_tag="pgaptmp_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HGU4703098.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(43351..43956)
                     /gene="ttdB"
                     /locus_tag="ACHKNP_RS04410"
                     /old_locus_tag="pgaptmp_000882"
     CDS             complement(43351..43956)
                     /gene="ttdB"
                     /locus_tag="ACHKNP_RS04410"
                     /old_locus_tag="pgaptmp_000882"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HGU4703099.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(43953..44864)
                     /gene="ttdA"
                     /locus_tag="ACHKNP_RS04415"
                     /old_locus_tag="pgaptmp_000883"
     CDS             complement(43953..44864)
                     /gene="ttdA"
                     /locus_tag="ACHKNP_RS04415"
                     /old_locus_tag="pgaptmp_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HGU4703100.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            45071..46003
                     /gene="ttdR"
                     /locus_tag="ACHKNP_RS04420"
                     /old_locus_tag="pgaptmp_000884"
                     /gene_synonym="dan"
     CDS             45071..46003
                     /gene="ttdR"
                     /locus_tag="ACHKNP_RS04420"
                     /old_locus_tag="pgaptmp_000884"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HGU4703101.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(46016..46633)
                     /gene="plsY"
                     /locus_tag="ACHKNP_RS04425"
                     /old_locus_tag="pgaptmp_000885"
     CDS             complement(46016..46633)
                     /gene="plsY"
                     /locus_tag="ACHKNP_RS04425"
                     /old_locus_tag="pgaptmp_000885"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGU4703102.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            46738..47106
                     /gene="folB"
                     /locus_tag="ACHKNP_RS04430"
                     /old_locus_tag="pgaptmp_000886"
     CDS             46738..47106
                     /gene="folB"
                     /locus_tag="ACHKNP_RS04430"
                     /old_locus_tag="pgaptmp_000886"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HGU4703103.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            47196..48017
                     /gene="bacA"
                     /locus_tag="ACHKNP_RS04435"
                     /old_locus_tag="pgaptmp_000887"
     CDS             47196..48017
                     /gene="bacA"
                     /locus_tag="ACHKNP_RS04435"
                     /old_locus_tag="pgaptmp_000887"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU4703104.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(48180..49418)
                     /gene="cca"
                     /locus_tag="ACHKNP_RS04440"
                     /old_locus_tag="pgaptmp_000888"
     CDS             complement(48180..49418)
                     /gene="cca"
                     /locus_tag="ACHKNP_RS04440"
                     /old_locus_tag="pgaptmp_000888"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HGU4703105.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPLTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(49482..50102)
                     /gene="ygiM"
                     /locus_tag="ACHKNP_RS04445"
                     /old_locus_tag="pgaptmp_000889"
     CDS             complement(49482..50102)
                     /gene="ygiM"
                     /locus_tag="ACHKNP_RS04445"
                     /old_locus_tag="pgaptmp_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HGU4703106.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            50344..51645
                     /gene="ygiF"
                     /locus_tag="ACHKNP_RS04450"
                     /old_locus_tag="pgaptmp_000890"
     CDS             50344..51645
                     /gene="ygiF"
                     /locus_tag="ACHKNP_RS04450"
                     /old_locus_tag="pgaptmp_000890"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HGU4703107.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            51668..54508
                     /gene="glnE"
                     /locus_tag="ACHKNP_RS04455"
                     /old_locus_tag="pgaptmp_000891"
     CDS             51668..54508
                     /gene="glnE"
                     /locus_tag="ACHKNP_RS04455"
                     /old_locus_tag="pgaptmp_000891"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HGU4703108.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            54556..55989
                     /gene="hldE"
                     /locus_tag="ACHKNP_RS04460"
                     /old_locus_tag="pgaptmp_000892"
     CDS             54556..55989
                     /gene="hldE"
                     /locus_tag="ACHKNP_RS04460"
                     /old_locus_tag="pgaptmp_000892"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HGU4703109.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(56109..56168)
                     /gene="ibsE"
                     /locus_tag="ACHKNP_RS04465"
                     /old_locus_tag="pgaptmp_000893"
     CDS             complement(56109..56168)
                     /gene="ibsE"
                     /locus_tag="ACHKNP_RS04465"
                     /old_locus_tag="pgaptmp_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HGU4703110.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(56485..56544)
                     /locus_tag="ACHKNP_RS04470"
                     /old_locus_tag="pgaptmp_000894"
     CDS             complement(56485..56544)
                     /locus_tag="ACHKNP_RS04470"
                     /old_locus_tag="pgaptmp_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4703111.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            complement(56783..58444)
                     /gene="yqiK"
                     /locus_tag="ACHKNP_RS04475"
                     /old_locus_tag="pgaptmp_000895"
                     /pseudo
     CDS             complement(56783..58444)
                     /gene="yqiK"
                     /locus_tag="ACHKNP_RS04475"
                     /old_locus_tag="pgaptmp_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
     gene            complement(58471..59100)
                     /gene="yqiJ"
                     /locus_tag="ACHKNP_RS04480"
                     /old_locus_tag="pgaptmp_000896"
     CDS             complement(58471..59100)
                     /gene="yqiJ"
                     /locus_tag="ACHKNP_RS04480"
                     /old_locus_tag="pgaptmp_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGU4703112.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            59369..59569
                     /gene="glgS"
                     /locus_tag="ACHKNP_RS04485"
                     /old_locus_tag="pgaptmp_000897"
     CDS             59369..59569
                     /gene="glgS"
                     /locus_tag="ACHKNP_RS04485"
                     /old_locus_tag="pgaptmp_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HGU4703113.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(59612..60677)
                     /locus_tag="ACHKNP_RS04490"
                     /old_locus_tag="pgaptmp_000898"
                     /pseudo
     CDS             complement(59612..60677)
                     /locus_tag="ACHKNP_RS04490"
                     /old_locus_tag="pgaptmp_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(60679..61428)
                     /gene="yqiH"
                     /locus_tag="ACHKNP_RS04495"
                     /old_locus_tag="pgaptmp_000899"
     CDS             complement(60679..61428)
                     /gene="yqiH"
                     /locus_tag="ACHKNP_RS04495"
                     /old_locus_tag="pgaptmp_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4703114.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            complement(61444..>63814)
                     /locus_tag="ACHKNP_RS04500"
                     /old_locus_tag="pgaptmp_000900"
     CDS             complement(61444..>63814)
                     /locus_tag="ACHKNP_RS04500"
                     /old_locus_tag="pgaptmp_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4703115.1"
                     /translation="RENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDN
                     QTAVCLTPEQLTLLGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQ
                     AWLKYKDANWTPPELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAW
                     RLRTDYQYDQSFNNGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFS
                     GVSLKSDENMLPPDLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFN
                     TLQGQLDVKVEEEDGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFF
                     WTGEFSWGWLNNVSLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQD
                     KETGYSYRANYSKRFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYI
                     VTFNQYLESLRLNTYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRIN
                     WEEDNQDQLYLNISIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGD
                     NDEFKDMKASLRASYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDY
                     SGSADSRFMIDADGAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVD
                     IENSVITTTLTEGAIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLV
                     ADGGFVYLNGIQDDSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
BASE COUNT        15537 a        15982 c        16708 g        15587 t
ORIGIN      
        1 attgacttac aataaatcta catctctaat acaccaactt tttggaaaca atattgtaag
       61 atcaaattta tatggtggcg aaaattaatc tcttatcacg gcttacgccg cttttattcg
      121 tgttcgctcc ttttttggca cagtcaaata tgactgttta tcctatggct gtttccatta
      181 atagtcaggg tgaaggcaac gttcgcgtaa tatctaaaag taatgaagtt cagtacatca
      241 aggcgacagt attccgtatc gataatccat cgacgcctca ggaaaatgaa gttgagatta
      301 aatccggtga tgcaaatcat ctggttgtta tgccacctaa attcgcttta ccagccggta
      361 gtagcaaaac cgtacgtttt gttgcgatgg aaccagagca aaaagagaaa aattatcgcg
      421 ttaaatttga agcggttccc agtattgatg acgttgccac agataaaaaa gatctctcta
      481 tgcagttaac agttaactta atttggggga ttgttgttag tgttccacct cagcaaccta
      541 ttgctaagtt agaagtaaat gctgcccaaa aattagttaa tgcagggaat caacgcttaa
      601 agattttaac gatcgcttat tgtaaaaata atagcaaaga aaattgtaag atacagaccg
      661 taaataaaaa catcttccct ggtcaggaaa gaaatcttga aagcatatct ggctacgaca
      721 aaatcgttgt caaatataat aactggatca ccaaagataa tggcgagttt gaactggcgg
      781 tccattaatt atcattgtaa taaacgaagg aatagtaatg aaaaaggtat ttgcaaaatc
      841 tcttctggtc gcagcgatgt tttctgttgc aggctccgca ttggcagtac aaaaggatat
      901 tactgtaacg gccaacgttg atgccgctct ggatatgaca cagaccgata acaccgcgtt
      961 gccgaaagca gtagaaatgc aatatctgcc ggggcagggt ctgcaatctt accagttgat
     1021 gaccaaaatc tggtctaacg acgttaccaa agatgtaaaa atgcagttag tttccccggc
     1081 gcaactggtt caaagcctgg atgctagcaa gattgttccg ctgacagtaa cctggggcgg
     1141 tgaagaaatt aaagctgatg cggcaaccac ttttaccgca accaaaatct ttgcttccga
     1201 tgcgttaact aacggttctc tggctaaaaa cctgatgttt gctcaaatca ccaaaggtgt
     1261 tctggagaca ggcatctacc gtggtgtagt aagcatttat ctgtctcagg atatctaatc
     1321 cagataacag aattaacacg taacaaaggg aggggtagcc ctcccttatt ttaactgttt
     1381 tagtatttgt tatggatttc aggattgcca tggataaaaa attactggct cttttgatcc
     1441 tggcgagtct cagcccggca gaggcgacat taaccaaaat tcccgcaggg tttgaggtta
     1501 ttgctcaggg acagcaggag tatatcgagg tttatttttc agggaaaagt ctcggtaaat
     1561 attatgcaat ggttaatctt gataccgtaa catttcttga tccagcaagt ctatataaca
     1621 agctggaact ggatgtagac gatcagaaaa tcgcgcatat agtgaaagaa aaattatcgc
     1681 agccgctagc tcgccacggt gaattggctt gcggttatgt acgtactgac tcagggtgtg
     1741 gttttctgaa taccgatacg ctggaaataa tctataatga tgaagaaagt tcggcaacgt
     1801 tgtttattaa tccgcaatgg aattcagctt tcgatgcgaa gtcattatat ttaaatccag
     1861 acaaaaatac tgttaatgct tttatacatc agcaagacat caatgttctg gcacaggatg
     1921 attaccaatc gttgtctatt cagggaaacg gtgcgctggg aataacagaa aatagttata
     1981 ttggtgcaca ctggaatttc aacggttatg atgcagatga tgtcagtgac agtaatgctg
     2041 acatcagcga tctctattat cgttatgatt ttttacgtcg ttattatgtg caggcgggtc
     2101 gcatggacaa ccgcacacta tttaatgcac aaggcgggaa ctttaccttt aactttctgc
     2161 cactcggtgc aattgatggg atgcgtatcg ggtcgacact gagctattta aaccaggcgc
     2221 aaagccagca aggaacccca gtaatggttc tgctttcgcg caattctcgt gttgacgctt
     2281 atcgtaatga gcaacttttg ggatcgtttt atctcaatag tggttcgcaa tttattgata
     2341 ccagttcctt tccgccaggc agctacagcg tggcattaaa agtctacgaa aataaccaac
     2401 tcacccgcac cgagcttgtg ccgtttacca aaaccggcgg tctgactgac ggaaatgcgc
     2461 aatggttctt acaggcaggt aaaactacat cacaggtttc tgatgatgaa agttcagctt
     2521 atcagctagg agtacgcctg ccattacatc cgcaatatga gctctacgca gggctggcga
     2581 atgccgatga tgtgagtgct ttcgagttag gtaataactg gacggcagat ttaggcgggg
     2641 tggggaatct tgcaatcagc gccagcgtgt tccgtaacga tgacggcggc aaaggtgata
     2701 tgcaacaggc caactggagt aatccgggat ggccgacgtt gggcttttat cggaccaact
     2761 ctgacggcga tgcttgtaca accgacagca gagagagcta taacgcctta agctgttatg
     2821 aaagtatttc cgcgacggtt tcacagaatt ttgtcggctg gaatatgatg ctgggttata
     2881 cccgcacaca aaataacact gatgatagtt tgcgttggga taaacagcag agctttgaaa
     2941 ataactatct tcgccagaca acagcgcaaa gtatctctga aactgtacaa cttagcgctt
     3001 cccgcgcttt tgtgatgcgt gactggattt tgagtacgtc agtcggtgtt ttccatcgta
     3061 atgacaacgg tggcgataac gacgacaacg gcttgtactt atcgttttcg ttatctgaca
     3121 cgccaacgat ggatagcaat aacaacagcc attcaacaaa tgtgtctacg gattatcgtt
     3181 atagcgaaca ggatggcgat caaacgtcat ggcagttatc gcataccttt tataacgatt
     3241 cattcagcca taaagaactt ggcgtaaccg tcggaggcct gaacaccgat accataaaca
     3301 gcgcggttaa cgggcgttgg gatgggcaat acggaaatgt ctacgctacc gtatctgata
     3361 gttatgaccg taagaatcat gatcatcttt cggcctttac gggcacttat agctctacgc
     3421 tggctgtcag tcgctatggc gttaatttgg gagccagtgg tacagacggt ttgctgggtg
     3481 cggtactggt ggatgtgaaa ggcttctctg aacaggatga agagagtcag gatctgcaac
     3541 tcgaagcgcg ggtggcaggc agccgaactt tgcagcttgg tcaaagtgac agtgtgttgt
     3601 tcccttatcc tggatttcag tctggttttg ttgaggttaa cgacagtagc cagggcaatc
     3661 agcaggggac aacaaacatc attaacggtg cagggaatcg tgaattaatg ttgttgcctg
     3721 gcaagctgcg ctatcgcgaa gtgtctgcca gctttaacta caactatatc ggtcgtttat
     3781 tactgccggc agcggtgaaa aaattcccga ttgtggggct gaacagcgcc atgttactgg
     3841 tagctgaaga tggcggattt acacttgaaa ttaacggcag cgaaaaagag ctctatctgc
     3901 tttccgggca gcaattcctt aagtgtccac tgagtgttgt aaagaaacgc gccagcattc
     3961 gttacagcgg agatgttact tgtagtgtgg tgacttattc acaattaccg gagtcgattc
     4021 aggttcaggc acagttgaaa cagcctaaat tacgtggaaa cgttcagacg gcgcaaaggg
     4081 aggttgcacc atgagaaatc gattgattgc ggcgatattg ggcttgtttg gttcaggcag
     4141 ctcctgacgt gaccagtgaa attacgtatg atttggcatc tggtagagcg gattattact
     4201 tctggaagga tgaagcgtcg gcaggaaata atggatatat gtggtatgaa tgttcgtatc
     4261 ctgacctcca acaaacctgt acagctaatg gaaatatatc gacagtacaa atctatttaa
     4321 ctgaacaacg cagtgggatg cgttggccgg taaaactcaa aggatttaaa acagccattg
     4381 taagtagcga tgaagcgccg ccaggatgca aggggggcaa agggcttcag acgaatctta
     4441 aggattctaa tagatcttca tgtacagaag atggtcaaca ttattatata tacgatacaa
     4501 agtttcttac gctctacctt gagcagacag agatgaagaa tttgccgatt ggtggcgtct
     4561 ggaaggggaa agttaaatta cattcgaaca gcccggccca ggactatttc gcaaatatta
     4621 ccctgaatac gctcgacccc aaccatattg acgtgttctt cccggagttc gcccacgcca
     4681 cgccaagggt gcagttagac ttgcatccaa caggaagcgt taacggcagc aactacgcgc
     4741 aagatctgac catgttggac atgtgcctgt acgatggttt taacggtaat gccatcagtt
     4801 atgaaatcat gctcaaagat gaagggcgac cagctgcagg gcgcagagac ggttacttct
     4861 ctatctatcg tcagggaggg accaccaccg acgagggaga acgcattgat taccgggtca
     4921 aaatgtacaa cccggaaacc ggtgggcaaa ttgatgtgcg caataatgaa aatatggtct
     4981 ggaacagcat taacctgaaa cgtgtgcgtc cggtggtact gcccggtatt cgctatgccg
     5041 tgatgtgtgt gccgacaccc ttgactctgg cagtcgataa attcagcgtg atggataaac
     5101 aggccgggta ctacatgggc aaattgtcag taatctttac gccttccttg ccaaccatca
     5161 attaaccctg caaaccggac aaacgctttt ttctatataa acgacgaagc cgtctgataa
     5221 agcggcttcg tcattacaac ttaagttaaa cgttaatgtt gcggtgcggg atcgttatgt
     5281 gtttcctgcg ccacctcaat cgccggtttg ttctgcaaca cggtccagat aaccagcgca
     5341 cctaacaggt cgaacactgc cagaactgcg aacagcgggc tgaagccgat ggtgtcagcc
     5401 agtgcaccga caaccagcgc aaacagcgta cttgccagcc atgcggacat cccggttaaa
     5461 ccgttcgccg tcgccacttc gttacgaccg aacacgtcag aagagagcgt aatcagcgcg
     5521 ccagacagtg cctggtgggc aaaaccaccg atacacagca gcataattgc gacatacggg
     5581 ttggtgaaca gaccgatcat acccgggcca atcatcagca cagcacccag cgttacgacc
     5641 atcttacggg aaacgatcag gttcacacca aaccaacgct ggaacagcgg cggcaggtaa
     5701 ccaccgagga tacaaccgag gtcagcaaac agcatcggca tccaggcgaa catcgcgatc
     5761 tctttcaggt taaagccgta aactttaaac atgaacagcg ggatccacgc gttaaaagta
     5821 ccccaggccg gttctgccag gaaacgcggc agcgcgatac cccagaactg acggttacgc
     5881 aggatctgac caacggacat tttcttcgcc gtgctcacct ggtgctgggc ttcctgacca
     5941 ttaataatat agtcgcgttc ttcatcggtc agatgcttct ggtcgcgcgg atgtttatag
     6001 aaaatcagcc atgccatcgc ccagataaag ctcaatgcac cggagatgat aaatgccatc
     6061 tgccagctgt gcattacgat tgcccatacc accagcggcg gcgcaatcat cgcaccaatc
     6121 gaagaaccta cgttaaagta acctaccgcg atggaacgct ctttcgccgg gaaccattcg
     6181 gagctggctt tcagacccgc cgggatcatc gctgcttccg cggcaccgac tgcaccacga
     6241 gcaatcgcca ggccacccca gctaccagcc agcgcggttg caccacagaa cacggcccac
     6301 agcacagcaa acattgcata accgattttc gtacccagca catccagtac ataacctgct
     6361 accggttgca tgaccgtata agcagcagaa taggctgcga tgatatagga atactgttgg
     6421 gtggagatgt ttaactcttc catcagagtt ggcgcagctg ccgccacagt gttacgcgtc
     6481 aggtaaccaa gcacggtgcc gagcgtcacc agtgcgatca tataccaacg taacccttta
     6541 attttacgca tgtaaaacct catcgttttg ttatatccgc aggaagcgcg gccatcatcc
     6601 cgccagaggc tgaagatgtt agttacgacc gggtaaccga aaaaaaaccg gcacgccccg
     6661 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     6721 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     6781 ataacttgtc atacaacttt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     6841 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagagaga tttaccactt
     6901 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     6961 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     7021 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     7081 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     7141 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggctgaa
     7201 aggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggccgagc gtctgtgtac
     7261 cggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     7321 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgtttg gtatcactgg
     7381 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     7441 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     7501 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     7561 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaacatcg aacaggcgac
     7621 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     7681 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     7741 gtctgaccat gcgctggacg tggtgatgtt tgctgaggcg aacgaagcgg aactggacag
     7801 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     7861 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     7921 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     7981 aggctttgac tccatcaacg accgtccgat ggcagaagag ttgtctaagc tgctgagcaa
     8041 gcagaacgaa gaaaacctgc tgccgaaaac cattctgtac tgcctgaacc cgcgcgataa
     8101 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     8161 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     8221 actggcgcag ctcggtttgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     8281 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     8341 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     8401 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     8461 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc ggatttggct
     8521 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     8581 ggacataaat ttgcgttaac ggatatcgca aaaggggcca atgtcatcaa atatggcctg
     8641 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     8701 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ctgattttca ggatctgcct
     8761 gcgcaagcgg cagatcgtga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     8821 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     8881 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     8941 agccacacct acggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     9001 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     9061 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     9121 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     9181 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     9241 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     9301 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     9361 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     9421 ctggtgacca tggtcaacga cttcaaacag tactttattg cccatgacca gccgatctat
     9481 gaaaacccat cgccggggaa caaagcgggc ggtatcacca cgctggaaga taaatccctt
     9541 ggctgtaccc agaaagcggg ttccagcgtc gtggttgacg tgctgcgtta cggcgagcgt
     9601 ctgaaaacgc cggggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     9661 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgtgg cacgccatac
     9721 ggcggctttg taccgacggt gaaaatcgct accaacagtg aactggcggc gaagaaaaaa
     9781 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     9841 gaagaattta tcgacaccat cgttgagttt gccaacggta aacaaacctg caacgagcgt
     9901 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     9961 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgata
    10021 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
    10081 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
    10141 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
    10201 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
    10261 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
    10321 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
    10381 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
    10441 gtcgataggc ggcacaatga ttgagggtag ttttctgctt atgaatggct tgaatatcat
    10501 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
    10561 cgccgtagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
    10621 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
    10681 tcgcaagagt cctgcaatac gccgatgata aagccgacgg caaccacctg catggcgata
    10741 tcgttcgaaa taccgaacat attacaggcc agcgggatca gcagcagaga accccccgcc
    10801 acgccggatg cgccacaggc acacagagaa gccaccacgc tcaacagcag cgccgtgggc
    10861 agatcgaccg ggatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
    10921 gctgcgcccg ccatattgat ggtggcaccc agcggaatag aaacggaata ggtatcgcga
    10981 tccagattca gcttttcaca cagtgccata ttcaccggaa tgttcgccgc agagctgcgg
    11041 gtgaagaagg catacacgcc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
    11101 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
    11161 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
    11221 agggtagaag aaaccagacc aaaaatacca atcggtgcga agcgaatgac cagtttcacc
    11281 ataaaggtga cggcattcga catatcgttg accaggtttt tggtggtctc gttaccgtga
    11341 cgcagtgcga aaccgaggcc aattgcccac accaggatcc cgatgtagtt acctttcagc
    11401 agcgcgtcga tggggttgga aaccatgctc attaccagcc cgcgcatcac ttctacaatg
    11461 cctgacggtg gcgaaatatc acccgcgcta ctggataagt gcagggtaga agggaaggcg
    11521 aagctgaaaa cgactgcggc cagagcagca gagaaggtac ccagcagata gaggaacaaa
    11581 atagggcgga tattggtttt ctgcccgtgc tggtggttag caatagatgc catcaccagc
    11641 atcaacacca ggatgggggc aacggctttc agtgcgccga cgaacaaagt acctaacaga
    11701 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
    11761 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
    11821 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
    11881 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
    11941 tcgggaagcg aaaaatgctg gattttaaag agaggtcata ttttgcaact taatggttac
    12001 atttatttga cataaaaata aagccaacgt gcggcttcca gactgatgac aacatcaaaa
    12061 aaggactcgg tcagtcccct cgccccgttt ggggagaggg ttagggtgag gggaacaggc
    12121 cagcatcggt gcgaaaaatt accctcaccc cgaccctctc cctggaaggg agagggagaa
    12181 aacggtgctc atgttaatga cagggtacac ggcttaggca gattaaaaat tatccacccc
    12241 gctgcttatc atgccgataa ttcacccagg cgttgataat aaacgtcatc accagaatgc
    12301 caaacaccac gcccagcgag acggcgattg gaatatggta gaagtcgaca atcagcatct
    12361 tgataccgat aaacaccaga atcaccgcca ggccatattt gagcatcgag aaacgctctg
    12421 ctacgcccgc cagcaggaaa tacatcgcac gcaggccgag gattgcaaac aggtttgagg
    12481 tcagcacaat aaacggatca gtggtcacgg cgaagatagc cggaatgcta tccacggcaa
    12541 aaatcacgtc gctcaattcc accagaatca gtaccagcat cagcggtgtg gcgtacagca
    12601 atccattctt acgcacaaag aaatgctcgt tgtcgatggt gtcggtcatg cgcaaatgac
    12661 cgcgtagcca gcgcaccagc ggcttgtcgc caatgcctga ttcatcttca tgggcgagcg
    12721 ccatcttcac gccggtaaac agcaggaagg caccgaagat atacaggatc cagtcgaact
    12781 gtgaaatcag ccagctgcct gtgaagatca tgatggtacg cagaacaatc gccccgagca
    12841 cgccatacac cagcacgcgg cgttgtaatg ccgccggaac agagaaatag ctgaacaaca
    12901 tcagccagac aaagacgtta tcgaccgcca gcgatttctc aatcagatag cccgtgagaa
    12961 aggccagtgc ctgtggatcg gcaacggcgc gaccttcagt ttgcaccaga taccaccaga
    13021 aagcggcgtt aaacagtaac gacagcgtca cccagaccag cgaccaggcc gcagcctgtt
    13081 tcatggtcat ggcatgtgcc ccacgacgcc cctgcaacaa caggtcgata gccagcataa
    13141 tggcgacaac aacagcgaat ccgccccata gcaacggcgt gccgacagta ttcatagaag
    13201 ttccttacac ataaaaaaac ggccaacgtc ggaagacgtc agccgctgct ttttatgcat
    13261 agacctcgcc ttccggcaag gtctcactta caacaaaaaa ggaatacgtc tgcgtattcc
    13321 tttcgggttg cccggtgacc ggatgtggtt tttcacacat cgtaatgacg atcgaccggc
    13381 aatgaagtta ctcccctttg caggtaacaa agtatgacag accattcagc cagtcaatgg
    13441 tctgttacat accttttaca ctgttttact ttggcaatta tagttttacg ctatctgccg
    13501 gaaaaatcac cccagtctgg cggcggatct cggtcagcag tttggcggtg atgcgactga
    13561 ccgccagccc cggatgatcc accagatgct catccaccag ggtagcgaac agctctgctt
    13621 cgtagagcat ggtattaata tgctgcggct gggtgagatc ctgcatttgg ctgccacgcg
    13681 gcacgaagca cactttctgg cattcagaca gtttttcaat caccagcgat cctgcttcgc
    13741 cctgaatctc gctcgccagg acagaatcac tgacttttga gtgctgcaag gtgacgctga
    13801 aatcaccgta atccatcacc accacgccgt gggcatctac accgcttgcc agcaaactgg
    13861 cggttgcctg cacgcttttc ggctcaccaa ataacgccac cgccgacgcc aggcagtaaa
    13921 agccgatatc cataattgaa ccgttagaga atgacggatt aaaggtgttg ggattttcac
    13981 cgtccaggta acgttgatag cgcgaggaat actggcaata gttgaaaaag actttacgca
    14041 atttgccgac tttcggcagc gcctggcgca gcaaatgaaa gttcggcagg caggcggttt
    14101 taaatgcctc aaacagcacc acctgatttt cccgcgcaca ggcaatggcg gcatccactt
    14161 ccgccagatt cgacgccagt ggtttctcgc aaatcacatg aattttatgg ctaaggaaaa
    14221 gttgtgtctg ggaaaagtgc agggcattcg ggctggcaat atacaccgcg tcaatggcat
    14281 cgctttccgc catcgcttcc agcgaggtaa acagatgctc gacagaaaaa tcattggcga
    14341 agtgctgagc ctgctcaagg ctgcgggaat atacggcggt taacttgtat ttaccgctct
    14401 catgggcggc ctcgacgaac tggcgagtga tccagttcgt accaatcaca gcgaaacgta
    14461 tcataacgcg tggatactcc ataaagggat tctgtcgtca atgtagcacg cctttttcat
    14521 cttcgacgtg cgcagatacg cattattctt ttgccggaca gccatgctca ctaaacggcc
    14581 caatcatgac cgatggcccg agatatttcg gctggcgatg gaggattttg gcgtcaatca
    14641 cggcgctgac acgagcgagt ttctcccgca gttgagtata aaaaccgtgc ttcatattga
    14701 ggtcaggcgc gttaaaaccg atagcgtcga tgccgtactg ttttgccagc cagatggcgc
    14761 gttgattatg aaattcttgc gagataatgg tgatatggtt ttcaccaaaa acctttttgg
    14821 ctcgcactac cgaatccagc gttgagaatc cggcatagtc gcagaagatc actttcgccg
    14881 gtacgccttt agcgatcaag cctgcatacc tgaggcttca tcgtaatttt tacgtccgtt
    14941 atcgccactt accagtagcc atttcacttt tccggcgtgg tacaacgctg ctgcggtatc
    15001 aatacgtcgg gtaaagtagc gattacccgg cctggccccc agcaataagc ccacgttacg
    15061 cgccggaact gcgttgacat cgctccaggt cagctgcttg ctggcgttga ccataacgcg
    15121 atcggcaata aaaatggtcg ctcctgctat aagcaacaac aaacagccaa tcttaagagt
    15181 ccggcgagaa aaacaaatac ggagaagaag gcggggctgc atggtcgtta tccttaacga
    15241 aaggggggag gtcgatttta ggcgctaaag aaagaaaaga taacgcctga catcattttc
    15301 agattttgtc ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg aactcaagct
    15361 ggtggtactg cggctccata tggcagcaca actggtaaaa cgctttgtta tgctctttct
    15421 ccttcaggtg cgccagttcg tgcaccacga tcatacgtaa aaacggttcc ggcgcgttgc
    15481 gaaacacagt agcgacgcgg atctctgctt ttgctttcag cttgccgccc tgcacacgag
    15541 aaacagcggt atgtaacccg agcgcgtttt ttagcacatg gatcttattg tcatacatca
    15601 ctttattgat cggcggggca ttgcgcagaa actgattttt tagatccgga gtatattgcc
    15661 agagggcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc
    15721 ccagacgctg ttcgttaatc agcgttcgca cctgggaaag tagctgctcg ggataaccct
    15781 gaagataagt aagattgctc ataaccgccc acgaaaaaga gaaaaagggt atactcacgc
    15841 acccttttca ggggcaacgt cgaaatttta tcattcagga gggccgatga gccacttaga
    15901 caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg ttaacccgct
    15961 acaggcgtgg gaagcggcgg atgaatattt gctgcaacag ttggacgaca cagaaatccg
    16021 cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca
    16081 taagccgtac agcattagcg actcatacat cagtgaactg gcgacgcgcg agaatttacg
    16141 cctcaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg actacccgca
    16201 acagccgggt gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg aacaacaact
    16261 gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg ccaaagcccg
    16321 tgacattcac acttccacgc tggaactgtt cgaaaaagtg ctcggcccga ccaccaccac
    16381 gctggcatgg aaaaaagcgc gcctgattaa ttgcacgttc aatgaaccgc cgctggccga
    16441 tgcgccgcag accgttagct ggaagctgga aggtactgac tggactatcc ataaccatgc
    16501 gaatgtcttc tcccgcaccg ggcttgatat cggcgcgcgc ttctttatgc aacatctgcc
    16561 agagaatctc gaaggggaga ttgtcgatct cggttgcggt aatggcgtta ttggtctgac
    16621 gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt
    16681 tgcttccagc cgtttgaacg ttgaaaccaa catgccagag gcgttggatc gcagcgagtt
    16741 tatgatcaac aacgcgctct ccggcgtgga gcctttccgc tttaatgctg tgctctgcaa
    16801 cccgccgttt caccaacaac atgcgctgac cgataacgtc gcctgggaga tgttccacca
    16861 tgcccgccgc tgcctgaaaa tcaacggcga gctgtatatt gttgccaacc gtcacctgga
    16921 ttacttccat aaactgaaga agattttcgg caactgcacc accatcgcca cgaataataa
    16981 atttgtggtg ctgaaagcag tgaagctggg gcgtcgtcgg taagattggg agttacctgc
    17041 cggatgcggc gttaacgcct tatccggcct acgttcagca cgttactccc aggccggata
    17101 agacgcggca agcgtcgcat ccggcaatgt ctgcccagtt ctggaatcgc gcttccataa
    17161 aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt tagcgttgtt caacgtttat
    17221 gctacatacg tgaagttatg acgcataaag aatacgattt ctttaccgct gttcatcgta
    17281 ctaaggggaa aaaatgattg cgattgccga catcttgcaa gcaggagaaa agctaactgc
    17341 tgtggcacct tttctggcgg gtattcagaa cgaggaacaa tacacccagg cgctggaact
    17401 ggtagatcat ctgctgctca acgatcctga aaaccccttg ctggatctgg tgtgtgcaaa
    17461 aataaccgcg tgggaagaat cagcgcccga atttgcggaa tttaatgcca tggctcaagc
    17521 catgcctggc ggtatagccg tgattcgtac ccttatggat caatatggtt taaccctttc
    17581 cgatctgccg gaaattggca gtaaatctat ggtgtcacgc gttttgagcg ggaagaggaa
    17641 attaacgctg gaacacgcta aaaaattggc aacgcgattc ggcatttctc ccgccttgtt
    17701 tattgattaa cgcgacgtgc cggatgcgtt gcatccggcg cttcaaataa cgatttaaat
    17761 ctccagcgcc agccgtgttc cctgggcaat tgcccgtcgt gcgtccagct ccatagccac
    17821 atcgcagccg ccaattaaat gcacagtttt cccgctatca atcagcggtt gcgccagcgc
    17881 gcggtttggc tcttgccctg cgcagatcac cacattgtcc actgctaata cctgcgtttc
    17941 gccgttgatc accacatgca gcccgtcatc gtcaatcttc tgataactta cgcctgggat
    18001 cattttcaca ccccgcgaga gcagggtggt gcgatggatc cagccggtgg ttttgcctaa
    18061 cccctgtccc ggtttgctgg ctttgcgctg gagcatcaca atttgccgtg ggctacgcgg
    18121 gatctgcatt ccctgcgggc ttaagccacc agcctgttgt aggctgctgt cgatccccca
    18181 ctcattacag aacctggcga tattctggct ggtggattcg cccggctgac ttaaatacat
    18241 cgccgtatca aaaccaatcc cgccacaacc gatgatggca accttgttgc ctaccggcgc
    18301 tttgtcgcgc agtacatcga gataactcaa taccttcgga tgatcgatcc cgtcgatagg
    18361 cggaacgcgc ggcacgatcc cactggcgag gatcgtttca tcgaacgcct gtaactgatc
    18421 cgccgtcacg gtgtgattga gtttcagcgt cacgcccgtc acttcgatca tccggtggta
    18481 atagcgcagc gtttcgtaaa actcctcttt gccggggatc tgtttggcga tattaaactg
    18541 cccgccgatc tcgctgtggg catcaaacaa tgttacatga tgcccacgcg ccgccgcgtt
    18601 aatggcaaac gccagcccag caggtcccgc accgaccacc gccagatttt ttttctgcac
    18661 ggcaggaagg attggcattt tggtttcgtg gcaggcgcga ggattcacca ggcacgaggt
    18721 gactttgcca acgaagatct gatcgaggca ggcctgattg cagccaatgc aggtgttgat
    18781 ctcatcggct cgccccgatt gcgcttttga cagcagttcc gcatcagcaa gaaacggtcg
    18841 tgccatcgat accatatcgg catcgccgcg cgagagaatg tcgtcggcaa cctgcggatc
    18901 gttaatccgg ttggtggtca ccagcggcag cgaaacgtgg cctttcagtt tgcgcgtgac
    18961 ccaagtaaat gcgccgcgcg gcacgggcgt ggcaatggtc ggaatacgtg cttcatgcca
    19021 gccaatgccg gtgttgataa tggtcgcgcc cgccgcttca atggcctgcg ccagctctac
    19081 cgtttcggca aaagtcccgc cgtcttcgac caggtcgagc atcgacagtc ggtagataat
    19141 aatgaagtcg ttgccgacgc gttcgcgcac cgcacgcact acttctacgg caaatcgcat
    19201 ccggttgcgg taatcgccgc cccactggtc actacgctga ttggtgcgca gcgtcagaaa
    19261 ttcgttgatc aaataccctt cggaccccat tacctctaca ccgtcgtatc ctgcctcccg
    19321 tgccagttgc gcgcagtggg cgaaatcgtc gatcagttgc aggatctctt catgggtgag
    19381 ttcgtgagga acgaaacggt tgatgggggc ctgcaatgcg gaggaagcga ccagatgcgg
    19441 ttggtagctg tagcgcccgg tatgcaaaat ttgcagggct attttgccgc cttcctgatg
    19501 taccgcttcg gtaatggtgc gatggtgtgg gatctggctg gcgtcgttga gcattgcgcc
    19561 accttccatg ccaacgcctg ttaaatctgg tgcaatgccg ccgctgacaa tcagcgccac
    19621 gccgtgacgg gcgcgttcgg cataaaacgc tgccagccgc tcggcaccgt ccgggtattc
    19681 ctccagcccg gtgtgcattg agcccatcaa cacgcggttt tttaacgtgg taaaacctaa
    19741 atccagcggg gcgaacagca acgggtagct cataaaattg tccagtatgt aaaataattg
    19801 ttatgtggtc ggatgagttc taatttagcc gtcgccggag aaaagggaaa aggggagtgc
    19861 ggggtttgtg atgggattca aaaagaatac gcccggtggt aacgccggat gcgacgcttg
    19921 ccgcgtttta tccggcctgg gggattgcac atgtagggcg gataaggcgt tcacgccgca
    19981 tccgccagtg gcacggtgca gatgccggat gcgacgcttg tcgcgtttta tccggccagg
    20041 aggattgcac atgtagggcg gataaggcgt ttacgccgca tccgccagca gttgcaaatt
    20101 attgcttacg gaaaaaatcg ttatacaaca tatacaaatg cgcggcactc caggagaaat
    20161 ttggtgcgcc ttgctgtgcg ccagtcagcg ggttgtaatt ctcctgaatt gggccatcgg
    20221 cggttaatcc tttggcgtga cggaagaacg tatccgccag tttcagggca tcatcacgat
    20281 aaccgtaacg ctccatccct ttcagaccaa accagaactg atccacccat acgcgcccgc
    20341 gccagtagat atcagcgcca aaagccggat tggttaacgc cgccgttccc agcgggacaa
    20401 aggtgttgaa ctctttagga tcgagcatca ccttcaccac cgcgtcggcg ttggcctgcg
    20461 ttgccgcacc gttaaacagc ggcgaccagc cttccggccc tttaccgcgc tcgacaatcg
    20521 gtttgcccgc gcagccgttc gccagcggtt tatcttcaat acgcacgtca tagtagaact
    20581 gggtagccgg gtcgaacata caggtgttta tgtagtctgc gagctgctgt gccaactggc
    20641 gatagcgttt ggcctcttcc ggcttaccga ggatggttgc catctccgcc agataatggt
    20701 tatcgctgta catatagctg gcctgatcca ccgactcctg caatagcgag tagcccagca
    20761 acgttccgtc ctgactgcgg ttttcggcga atttcaccgt ccagtcgcta cgtttgctgc
    20821 cattagcgac atatttatcc aactgttctt tgtcgataaa accaaagacg gcggcatcat
    20881 cacgccccga ttcccacgac gcagcgacct gtgccggaat ttccagactg tcgtactggc
    20941 ctttctccac cacgcgggcg tagttgttca gcccagactg cgtctcttct ttgttgcctt
    21001 tcttcaccgt aaacagcatc tcgccgctct cagtgttgtg ggctttgtcg cgggtcgcgc
    21061 catattacgg cacgccgttg ccgttgtgat cgcggttacg taaccaccag tcgtgatagg
    21121 ccaccagttt cgggtacatc tctgccaacc aggctttatc ctgggtgacg ttgtacactt
    21181 ccatcaccga ccaggcggca aggctgggtt tggtattacg ttcgttccag ttgccgccat
    21241 cgccaccacg ctcggggcta aggttccacg ctatcaggtc ggggacaaag cccacatcct
    21301 gcggacgcac gctatcgcca ggctggatct gccaggagaa gaccgcgcgg atattctctt
    21361 tggcgatgtc cggattgaaa tgcgccatcg caaacgcctg cttccaggta tcccacggcc
    21421 aggtctgatt gccggagaac cagcgcccgg tcaccgacgg tgtaacggtg ttaaatttca
    21481 ccgcaccgcc cggtgagcgc cagttaccgt tgagcgtttc gatcgctttc accgcgacgc
    21541 gcgtctgttc cggcgtcgca tccggattgg ttaacccttt cttcagatac tcttcccagc
    21601 gttgctgcga ggcggtgaga taaaacgccg gacgcgccag aatatcgcgg atctgcattt
    21661 gctctttgct aacttcctga gaggtcagca gatgggaata ggtggtatag agcgtcgtcg
    21721 aaccgttgat atgtgcctta ctggtaaagc gattgccgtt gatttcagtc tgcaccggca
    21781 gggatttatg cacctgatat tccgattcgc cggaggtcag cagatcccag gtggcgcgca
    21841 ctttgccaaa ggtgactttc aggccatcac gggtggcgct gattttgcgc tggtagtcag
    21901 ggtattcgcc agcaatggtt ttatcggaaa gcggtttccc ttctttcgct tccagttttt
    21961 ccagcagttc gccgtcccac accagatcca gcggtttatt gctggtgatt ttggtttcca
    22021 gtagtgacgt gcgcggcgtg gcgaagcgca gagtcatttc gacttgcaca tcttttgctg
    22081 tcagtttttg caccagcgca ccgggaatac tgtatgcctc cagcgtaaag tcgactttct
    22141 tgccgtcctg ccagacggtc aggcggtcga aattgctggc cataaagttg atgtactctt
    22201 ccgtcagcag cgcaacgccc ggaaagccgc ccatggtgtt agggccgtct ggcaacagat
    22261 gaccatgcca ggctccgaga tcgaaaaacg gattaaaacg ctggtgatcg tcgtaatcgt
    22321 aatccttcat gtactgcggc gcgccagtac ggttaatcac gtttttataa ttgtcggcgt
    22381 tagcggcatg ggcggaaaaa cttatcagca gagcgcaggt tactggcgtt aaaatagttt
    22441 ttattttcat gaaattacag tccttattac cagtagaaat attgcatcag gaagatttgg
    22501 tcgctgggat cgtgagaggc atttttcatg tagaagcggc tatcaaaagc cgtggcgaaa
    22561 cgtccgccgc cgtattcgta ccagatacca aactgcatga aagaggtggt ttcatcgtca
    22621 gcgtggtcag ggcgatgttt ggcgtaggtg taagcggtgg agagataaac gccctttaac
    22681 gcttcataca tggcgctcac catcatccca ctttcacttc ccggtacgtc cagcccgtcg
    22741 gcgcgtccgg tatggtgcca ggcgcgagcg gcaaagttag gagttagcag gcccgtgaga
    22801 tagagctgtc ctgtgccgtc ggtgatttcc agaccgttca tccaggtgat gccttcggca
    22861 aggtcatact ggatgtaacc gttcatcatt gccggatagg tgtatttgtc gtcatagcga
    22921 tcgtacttgc caaaatgtag ccaggctttg ctttcgtcat gatgtccggc aggtgtggcg
    22981 gtgacgctat aacgaaagtt gccgcttaag ttctgcacct tcagcgcggt cataatgtcg
    23041 cgggtattgg gaatgacgta gcccagatcg ggggtgaagt cgccccacca ttgcaggtca
    23101 tccagcgagg aatcctgacg cagactgagc atgatttcgg tgccatcatt ggtacgataa
    23161 ccgccataga aacggttaat tccgccctcg aaaccacccc agctatggtc tggcacccag
    23221 gcattgccgt cgtggtcggc ctgcacggtc cagccttcgc cgtaaatcag gccgaaccag
    23281 gagccgtact tcatctccag gccaccttca atataggtac cgtcgctgta tttatgttta
    23341 ttctcaccct ctaaatccat atatccacca acccctaatt cgccgtaaaa acgaaatgaa
    23401 ggagaagatg tcgaggtaat tgcaggtgtt tttgtgtcgt catgttctgc cggacgtgct
    23461 tcttctcctg ccgcagaaaa ggcgtagcaa aacggcagag caagtaatgc tctgcatggt
    23521 gcagtgatta atttcattgt gttaatccat gagctaaaga aaaaatacag ccttttatcg
    23581 ttgtaattat aaaaatataa aggcagaaac tattttccct ctcctcatcc aggaagaggg
    23641 aaaatgcctg ctcatttttt attattctgc ttaatcaaca ttgctggctg gcgtcgcttc
    23701 aatatggcgc tcttcagccg ttaatccctt tatatattta ctgtatttcc accacgcaaa
    23761 gccaaggaag ataacaatac cgccgacgtt ataaaaaatg atggtcagaa tattcgcgcc
    23821 agtcgggaat gtcgaagcga caaaccctac ggcaaatatc gcaatcagca ttgaaacaac
    23881 gataataccg gtgcggcggg agcccatacg gaaatcgcgt ggcaggtgat ctaatttggc
    23941 gcgtaaattc aggtaagcca gcatgatgaa taacggcgga agcatggacg ctgcggcggt
    24001 catattaata atagtattca tcagatcctg cacggtattg gaaccgagca tcgggataat
    24061 catcagcggg atgacgatca ggaactggat ccacgctgcg cgcgccggaa cgccgttttc
    24121 gttcagttcg acggttttct taccaaagat gccttccggg atttcggaga agaaaatttt
    24181 caccggcgtt gcggtccaca tcagcaggga accgaacatc gcggtaaagg acaccagacc
    24241 gacaaagcga ttcatcaacg cttccggtag accaaaatac gccgccatgc cgtggaatac
    24301 ctgcaccgat ccgacggtaa atttcaactc tttgctgctg acgaagacgt taatcagcac
    24361 cgaggagacg gaatacagta cgccgataaa aatcccggcg aggatgatca ctttaacgaa
    24421 cgatttcgaa ccgcctttga cgtcgttaac gtacacagcg acggactccg cgccacctgc
    24481 ggccataaag atccaggtgg taacgccgag gaacgcccag ttgaagttcg ggatcatcgc
    24541 gtcaacggtg atggggtcag caggctgtac gccgccaacc agcgccgtac ctgccagtaa
    24601 aatgtaggag agcgttaaca gcagcatcag cgttgaagtg acggaggtaa ttggccccaa
    24661 cattttcgcc ccgttggtgg aaacccaggt ggagaaggcg aacagcacca tactgataat
    24721 ggtggtggca accggcgtca taatatattc gtatccgagg aaggcatacg aagcataagc
    24781 aataacgcgc ggcaacagtg aggtgaaaaa gaacaggttt acgaaccagt aggtataggc
    24841 agtaataaat gcccaacgtc cgcccagcga actttttacc cacgcgtaga caccggcttc
    24901 tgagttttta tttaacgaaa caaattctgc gatgatcaga cagaagggaa taaaataaaa
    24961 aatcgtcgcg aggaaaaaca tcggtgccga ggccagtcca agctcaatat tattattgat
    25021 aacgttgtta aagctgtaaa cggcggcaaa agtcagcgag agcaagccga atttgccgat
    25081 tgtattacgt ttggtatcag acataacaca tctccgaaaa tgtgtagggt agcggtggcg
    25141 gcaaactcct tttgccgccg tagttgttat ttgttatgga aataaccatc ttcgatggtg
    25201 actttgagaa ccactttttt gaccgcgtta ttgcagataa accgatacgc ttcatggata
    25261 tcgcaaataa cgatttgccc ttcgtggacc tcaacggttt ctccgcagcc ttttaaatat
    25321 tcacggtcag tttcatcacg ataatattcc actacctgta atgtctcttt cggtgcatat
    25381 tcaatttttt gctgcccttg caggtagtaa tgcacttcaa aatagcgtcg atgcccggta
    25441 aatagcgcat cggtcgcgga gtctgtctcc acgcggtaag tcaatgagtc accgatggag
    25501 tgggcgacgc caggctgaat gttgtcgata ttttcaatcg cttcaacgca gcgttgccac
    25561 ttcttgccag aggcgtaaat ctggcggaac tgttctaagt tatcgatgat cctcattgct
    25621 tattctcgct gtgcaaattc gtggaaaaga accctgcgcc gaactcatac gacgccaggc
    25681 tttgcgcggt agcttctccg ccagaaaccg gcagcaacgt aaagccgtag ctgaagtcac
    25741 ggaaccagac gcgccaggag tccagcacct cgctgcccca ggagttggag ccgaggccaa
    25801 gtagctggtg gtcgaggttc agggtgatgt catcactgcg ctgcagctcg ttacagtgct
    25861 gggcagcgtg gatgttttcc tgggtatagt gccaggcgct gaagttaatt gggcgctgcg
    25921 gaaccaccag cagaccgttg ccgtggcggt tagtcagtgc cgtccagcgg acatgctgac
    25981 ggttgccgtt gttctgcggg aagggatagt tctcgaacat ggcatcgacg gtgctgcgcc
    26041 agatatcgat gatgttagcc tgctggctgt cggcgtagtt ttcgcccggt ccacgaccgt
    26101 aatacgccac ctgatcgtat tcgccgttaa tccccatggt gaaaccgatg cacggaatga
    26161 tgtgcggata gtcgccgtaa cgctcgccgg aaagcgccac gttaacctgg ccatcggcag
    26221 cgatgcgcca gatgtaggtg cagcgcatcc cgaagtcaaa caccggcggg gcaataactg
    26281 tgcggctgat gatcaacact tcaccatcgc tctgttctac ggcaaagtcg cgcagatgtt
    26341 cctgcatgat ctgcaaatga ttcggttgcc acagcccttc gttctcctgc ttgtggttgt
    26401 cgatcattgg cttgaagaag ttgatctttg gctcgcgggt cagcagcgac tcgccattca
    26461 cctgccagga tgtcggtttg ccactcattt ttgagaaggt gatcgcgaag ttgtagccgc
    26521 gaacggtgca gctcaaacga tcgtcttcca gcgtcagcgg acgcgcatta tttggtgcga
    26581 aaggcactgg ttgcgcggtg ttttccttca gcgggaactg ataagtggcg atagaatgcc
    26641 cggcttcgct gtagcgggtg cgggaatctt tggtcaccgt aatgttgagg aacgcttcgc
    26701 gggcgtccag ctgcggcagc gtgatctgca agggggcttc gctgttcggc gcaacgtcgc
    26761 gcagtttaat ctgctgcgtc gcgagcgttt caccttcgac gcgcacctct gcgtgcaggg
    26821 tgtagtcatc aagcgtggta aaccacagtt tattttcgac tttcagctcg ccgcgagtca
    26881 gatccagcgc gtggattttt accggcgcga taacctgttt gtactctttc aggcccggtc
    26941 ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg ttgggatagt
    27001 cgccgtagtc gccgccgaat ttataccaga cgttgccgtt gtcatcctgc gcctggatcc
    27061 cgtggtcgca ccactcccaa acataatgtc cctgaataca atcgtgctta tagaagacgt
    27121 tctggtactc cgtcagcccg cctggtccgt tccccatcgc atgagcatat tcacagatga
    27181 tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg cgggtgtaca
    27241 tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatgc accagtcgcg
    27301 tgtcatccag cgccttcgct gcgtggtaca tcgcgcggat gttacagcca tagccggatt
    27361 cattgcccag cgaccagatg atgatcgacg gatggttttt ctgcgcgtga atatggcgaa
    27421 caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg ctgatatcgc
    27481 cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac aagccgtaga
    27541 tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc acggagttga
    27601 tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca acggcgcggc
    27661 ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa cggttattga
    27721 tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt ggcaccactt
    27781 ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga taaggggatt
    27841 ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg gtcagttttt
    27901 caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca aacaaggtat
    27961 attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact tcgcaggaaa
    28021 gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg ttaatatgcg
    28081 ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgctgaccac cacatatcct
    28141 ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac aggttgtcgc
    28201 cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc ttgctgaaac
    28261 ccacatactg accgttaaca tagacttcaa aataggtttc gacgccgtca aatttaatca
    28321 gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag gcaccggttg
    28381 ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg tcggtatatt
    28441 gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc ccccagtcag
    28501 ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa aagtggaaat
    28561 tccactgacc gcttaagggc agaaacaggc tgctggtttc gcgggcaaag gtacgcgctt
    28621 gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt tcgtgggtga
    28681 gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg gaaccattca
    28741 tggtgatggt agtttgtgta attgctgggg agtttagtaa aatttttact aaaaagtagc
    28801 aggtaaatgc atttacttta actggatcac gaaaataaac ggcagcactg tccggcaaaa
    28861 acgattgccg gacagtgtgt aaggggattt aacgggtcgt gccgcgcagt tttaatttgc
    28921 tgggaacgaa gactaacagc ggcagcgcgc gaccatcgcg ggctttttca tacaccaggt
    28981 taacgccctg acttcccatc atctcggaat ggatgcgcac ggtggagagc ggcggaaagg
    29041 taaatcgcgc ggtggggata tcgttaacgc tgataagcga aatatcctgt gggatgttca
    29101 ggcctcgttc atgaattgcc cgcagtacgc cgatagcaat ggaatcggaa gcaacaaaca
    29161 gtgccttcgg atagtcttcc cgcgccagca tttgttttgc cagttcataa cccgacgaac
    29221 tggaaaaacc gccgcgccag atatcctctt cgcgtaccac ttgtttcagt cggccatatt
    29281 ccgcaaaggc gacctcacga atatccgcct tgccaggctc atcttcaccg ccaataaaac
    29341 caatacgatt aacgccctgg ttgatataga agtcgatgat ttctttactg atgcgtgcca
    29401 gatcgatatc caccgcatcg taaccgctgc cgggttcgtg aaagtcgata aaacagatat
    29461 tgtcggtcaa cgcgctggca gcggcgcgca gggcgggcgt gggtttgccg acaattaaaa
    29521 taccggtgac gtttttaatg tctggtaagc cgctgtgttc ataacagttg gtgagctcga
    29581 tgcccagctt ttcgcactgg gtttcaatgc cgtggcggat cgccagatag taaggatcgt
    29641 tgatctccag ctcctgctgg tagctgtaga tagccagaat atggtgttgg ttgactgcac
    29701 ctgtctggag tttacgggca ctactggtct tgtactccag cttttcggcg atctcgagaa
    29761 tgcgatgttt cgtctcttct ttcacattca atgtcggatc gtcatttaag accctggata
    29821 ctgtcgccag ggatacgcca gcttcgattg cgatgtcttt tagtgttgcc atgtttacct
    29881 tttttacttt ccctgatgtg cgcccccatt gtagaggaag tgcttatcgg gtcacctgtt
    29941 ttcagtaaat aaagcgcgtt tctttgacgc tgtaacggta acacagcggc tcacgaatgt
    30001 gatcacgcct gcattctcca tttttagcct acaggattga gaaaaacttt tgctatagtt
    30061 tagtgaaatt ttacccgaag ggaggaaaca atggaaaccg tggcttacgc tgattttgca
    30121 cgtctggaaa tgcgcgtcgg aaagattgtg gaagtgaaac gccatgaaaa cgccgacaag
    30181 ctgtacatcg tacaggttga tgtgggggaa aaaacactgc aaaccgtgac cagcctggtg
    30241 ccgtactaca gcgaagaaga gctgatgggg aaaacggtgg tggtcttatg caatctgcaa
    30301 aaagcgaaaa tgcgtggtga aacgtcggaa tgcatgctgt tgtgtgcgga aaccgatgac
    30361 ggcagtgaaa gcgtgttgtt aacgccggag cggatgatgc ctgcgggcgt acgcgtcgta
    30421 taaaaagatc ggatggcgac gtcgtatcgc catccgattt gatattacgc ttcttcgaca
    30481 cttactcgca tggccgccag cgccttacgc gccgctttga tcaccagttc acactgttca
    30541 atggtcagtg tcagtggcgg ttcaatgcgg atcgttttgg cgttattgag cgttccggcc
    30601 accagtacgc gctggcggaa catctcgctg gcaaagttat agccgatttc gttatcaaca
    30661 aactcaattg ccatcaacat ccctttacca cgcgcttcct gtaccagatc gggatattcc
    30721 cgcgccagtt gacggaaacc gtccagcaac atatcgcctt tttgctcagc ctgagccggt
    30781 aagttctgct ccagcaacac attgatggtc gccagcgccg ccgcacaggc cagcgggttg
    30841 ccgccaaagg tggtggtatg caggaatggg ttgtcgaaca gaactgaaaa cacctcttca
    30901 gtggcgatgg tcgcgccaat cggcatcacg ccgccgccga gcgctttggc aaggcagagg
    30961 atatccggct gtacgttctc atgctcgcag gcgaacatct tgcccgtgcg ccccatgccc
    31021 gtttgtactt catcgaggat catcagtgcg ccgaactcat cgcatagctt acgtacggcg
    31081 gtgagatagc ccggcggcgg cagaattacg ccaccttcac cctgaatcgg ttcgaggatc
    31141 accgcagcca catcatcacc ggtttttttg cactcgttaa gagccgtgcg catggcttcg
    31201 atattgccaa acggcacatg acggaagccc ggcagcagtg gcataaacgg tttgcggaag
    31261 gtcgatttcg ccgtggccga cagcgcgcca agtgatttac cgtggaacgc gccgctggtg
    31321 gcaataaaag taaacttgcc gcgcggtgac tggtaagctt tcgccagctt cagcgctgct
    31381 tcaacggact cggtgccgct attacagaag aagctgtatt tcagtttacc gggcgttagc
    31441 gcagcaaggg ttttcgccaa catcgcccgt aacggatcga gcagctcctg gctgtgcagc
    31501 ggttgtttcg caagttgatt ctgtacggcg gaaaccacaa ctggattacg gtgccccacg
    31561 ttgaaaattc caaaacctcc caggcagtcg ataaactcct gtccctgggt gtcgacaagc
    31621 gtatttaaac ctcccgcttg ccactctacg gctccgtaat ccccgccggc ggtaacagat
    31681 ttgcgatact ctaaaaaccc cggattgaca tgctctttga agtattcaat cacctctcgg
    31741 ttaagtgctt tcatctcctc atgatccagc gttcgcttct caatgagatt cagggcgtgg
    31801 gcgctgcacg ctaaagccga tgcgctcgaa ggtaacctgt tcaaaatatg ctccggaggc
    31861 tcgcgtatca catgataccg atttaagtat tgcagggatt gcgccactcc ggctgcgatc
    31921 gcgaaaatcg ggataaacac aaattcggtt atcatttgtg caatatttaa tgctatctgt
    31981 taacatttgt tgttattttg cacggttttt gtcgggtatg gatttgatga ttgctgcgca
    32041 aatgcacgtt ttcagggcaa taattgcctg atttttgggc aagataatta acgttttcgt
    32101 atagtaacag agtgaaagta ttttaaatca aagaattaga tttgtgcttt aatcgcaaat
    32161 tgcgatctaa atcaaattaa tcggttaaag ataaccgcag cggggccgac ataaactctg
    32221 acaagaagtt aacaaccata taacctgcac aggacgcgaa catgtcttct catccgtatg
    32281 tcacccagca aaataccccg ctggcggacg ataccactct gatgtccact accgatctgc
    32341 aaagctatat cactcatgct aatgacactt ttgtgcaggt gagcggcttt accttgcaag
    32401 agttacaagg gcagccgcac aacatggtgc gtcacccgga tatgccaaaa gcggcgtttg
    32461 cggatatgtg gttcaccctg aaaaaagggg agccctggag cggcatcgtg aaaaatcgcc
    32521 gcaaaaatgg cgaccattat tgggtgcggg ccaatgcggt accgatggtg cgcgagggaa
    32581 aaatcagtgg ctatatgtcg attcgtaccc gggcgacgga tgaagagatt gcggcggtgg
    32641 agccgctgta caaagcgctg aacgccggac gtaccagtaa gcgtattcat aaaggcctgg
    32701 tggtgcgtaa aggctggctg ggtaaactgc cttcattacc gcttcgctgg cgggcgcgtg
    32761 gagtgatgac cctgatgttt atcttgctgg cggccatgct ttggtttgtt gctgccccgg
    32821 tggtgacgta tttcctctgt gtgttagtgg tattgttggc aagcgcttgt tttgaatggc
    32881 agattgtgcg cccgatagaa aatgtcgccc gtcaggcgct gaaggtggcg accggagagc
    32941 gtaatagtgt tgagcacctg aatcgcagcg atgagctggg gctgacatta cgcgcggtag
    33001 ggcagcttgg cctgatgtgc cgttggctaa ttaacgatgt ctcaagccag gtgtccagtg
    33061 tcagaaatgg cagtgagacg ctggcgaaag gcactgatga actgaacgaa catacccagc
    33121 agacagttga taacgttcag caaacggtgg cgaccatgaa ccaaatggcg gcgtcggtga
    33181 aacagaactc tgccacggcg tcggctgccg ataaactttc tatcaccgcc agtaatgcgg
    33241 cagtgcaggg tggggaggcg atgaccacgg tgatcaagac aatggacgat atcgccgaca
    33301 gtacccagcg cattggcacc attacttcgc tgattaacga tattgcgttt cagaccaata
    33361 ttctggccct gaatgcggcg gtggaagcgg cgcgtgccgg cgaacagggc aaaggttttg
    33421 cagtggtggc aggggaagtg cgtcatttag ccagccgcag cgctaatgct gccaacgata
    33481 ttcgcaagct gattgatgcc agtgctgata aggtgcaatc cggttcgcag caggtacacg
    33541 ccgccggacg gacgatggaa gatattgtgg cacaggtgaa aaacgtcacc cagttgatcg
    33601 cccagattag ccattcaacg ctggaacagg ccgatgggct ttccagcctg acccgtgcag
    33661 tggatgagct taacctgatc acccagaaaa atgccgagct ggtggaagag agtgcgcagg
    33721 tgtcggcgat ggtgaaacac cgcgccagcc gactggaaga cgcggtgacg gtgctgcatt
    33781 aatcggtatg ccggatccgg cggttggagc acaatgcctg atgcgatgct ggcgcatctt
    33841 atcaggcctg cggggtgtgt agcgggtgta ggccggataa ggcgcgtcaa gcgtcgcatc
    33901 cggcaattgc accgcgccac tggcggatgc ggcgattaat ccagttgcgt aatatcaaaa
    33961 gcggcgcggt cgatcaccgc aatgatcttt tttatttgtg catcactgat atcgctctgg
    34021 ttgacgtgta aatccagcac cgctttaaaa ttatccagcg cccgcttcat ttgcgggttc
    34081 tggcgcagcg ccgcgccaac gcaacgcgct ttgatgcgtt cttcaatcat ctccacctgt
    34141 tcgcggtttt cttccagcca ctgcgcgcct tgttcggtca gcgcaatctg cttcttacca
    34201 ccttcctctt cgcggatggt aatcagcgac tgctcctgca gaaaatccag cgttgggtag
    34261 atgacgcccg ggcttggggt gtaattcccc tgggttaggt tctcaatcgc tttaatcaat
    34321 tcgtaaccgt ggctgtcatc gcgcgagaga atatccagaa tcaccagacg caattcaccg
    34381 tgaccaaaga aacgttgccg acgaccgccg ccgcgaccgt ggcgaccacc gcagcattga
    34441 ccgtgttcat gctggtgacc gtgtccgcag tgctcgtgtt ctgacttctc acctttgcag
    34501 cagccctcat ggcgtggctg gccttcatgt ttacaacacc cttcgtgatg atggctcata
    34561 tcagcctcct tttgcttgat tagatatatc taatttatat ctgatgtgag cattactgca
    34621 agtacaaaaa tgatttttaa acgtggaagc acctcacatc caggccggat aagacgttca
    34681 cgccgcatcc ggcagcattg ctatggcact gaatgccttc acatacttca gatatatcag
    34741 ttctacaaat cgcttgcatt tatcatgatt aacaatcatt atcatttacg aaaatcattt
    34801 agatatatct gattactatc acgaaggcga taacaatgaa taactccccc cgctacccgc
    34861 agcgcgttcg caatgatctg cgcttccgtg aactgactgt gttacgcgtt gagcgcatca
    34921 gcgccggttt tcagcgcatt gtcctcggcg gcgaggcgct ggacggtttt acatcgcgcg
    34981 gctttgacga tcacagcaaa ctcttctttc ctcaacctga cgctcacttt gtgccgccaa
    35041 cggtaacgga agagggcatc gtctggccgg aaggaccacg cccactgtcg cgtgactata
    35101 cgccgctgta tgacgaacta cgccatgaac tggcgatcga tttctttatt cacgacggtg
    35161 gggtcgccag cggctgggcg atgcaggcgc aaccgggcga taaacttacg gtggcaggtc
    35221 cgcgcggttc gctggtggtg ccggaagatt acgcgtatca gctgtatgtc tgcgatgaat
    35281 ccggaatgcc cgcactgcgc cgccgcctgg aaacgttgag caaacttgcc gttaaaccgc
    35341 aagttagtgc gctggttagc gtgcgggata acgcctgtca ggattatctc gcgcaccttg
    35401 atggttttaa tatcgaatgg ctggcacatg atgagcaggc ggtagatgcg cgtctggcgc
    35461 agatgcaaat ccctgccgat gattacttca tctggataac cggcgaaggt aaagtcgtta
    35521 agaatttaag ccgccgcttt gaagcggaac agtatgaccc acagcgggta cgtgcagcgg
    35581 cttactggca cgcaaagtaa ctgatatata aaaagaaaaa ggctgacgat ttctcgtcag
    35641 cctttgctat atctggtggc ccctgctgga cttgaaccag cgaccaagcg attatgagtc
    35701 gcctgctcta accactgagc taaggggcca tggtagcgga ttataaagta actccgtgtc
    35761 gcaatccagc cattaccgtg cgcctgctgt ttttataaac aatgtatttt caatccgtta
    35821 tacttttcct ggtgatgtta gaaaggagta aatatggttg aggatatttt ggctctaggg
    35881 ttacgggtcg tgttttgcgg tatcaaccct gggctttcat ccgccgggac tggttttccc
    35941 tttgctcatc cagcaaatcg cttctggaag gtgatatatc aggccgggtt taccgaccgt
    36001 cagttgaagc cgcaggaggc acagcatctg ctggattatc gttgtggcgt caccaaactg
    36061 gtagaccgtc caacggtgca agccaatgaa gtttcaaagc aggagctgca cgcaggtggg
    36121 cgtaagctga ttgaaaaaat tgaagattat cagccgcagg cgttggcgat tctgggcaaa
    36181 caagcatatg aacagggatt cagccagcgc ggtgcacagt gggggaaaca aacgctcacc
    36241 attggttcga cgcagatttg ggtgctgcca aatcccagcg gtttaagtcg cgtttcacta
    36301 gagaaactgg ttgaagcata tcgcgagctg gaccaggcgc tggtagtgcg tgggcgataa
    36361 aaaacgccac cgaaaaggtg gcgtttgtgc aaagggacta gtgccgggtg cggcgtaacg
    36421 cctgatccgg cctaccgatt aatcgtccag gaagctacgc aacacttcag aacggctcgg
    36481 gtgacgcagt ttgcgcagcg ccttcgcttc gatctgacgg atacgttcgc gggtaacgtc
    36541 gaactgttta cccacttctt ccagcgtgtg gtcggtgttc atatcgatac cgaaacgcat
    36601 acgcagaact ttcgcttcac gcgcggtcag gccagccagc acgtcgtgcg ttgccgcacg
    36661 caggctttcg gtggtcgcag aatccagcgg cagctcgagg gtggtatcct cgatgaaatc
    36721 ccccagatgc gaatcttcat catcaccgat cggcgtttcc atggagattg gctctttggc
    36781 gatcttcagc actttgcgga tcttgtcttc cggcatcagc atacgttcag ccagttcttc
    36841 cggcgtcggt tcacggccca tctcttgcag catctggcga gaaatacggt tgagtttgtt
    36901 gatagtctca atcatatgca ccggaatacg gatggtgcgc gcctgatccg cgatagaacg
    36961 ggtgatcgcc tgacggatcc accaggttgc gtaggtggag aacttgtaac cacggcggta
    37021 ttcgaattta tcaaccgctt tcatcagacc gatgttgcct tcctgaatca ggtcaaggaa
    37081 ctgcaagcca cggttggtgt atttcttggc gatagaaata accagacgta agttcgcttc
    37141 aaccatctct ttcttcgcac ggcgggcttt cgcttcaccg atggacatac gacggttgat
    37201 atctttaacc tgctcgatgg tcaggccggt ttcttcttca atctgctgca gtttctgcag
    37261 ggcgcgatgc acttcttcag agacatcgtg cagtttttcc gaccacggct tgttcatcgc
    37321 aattgccgcg ttgaaccagg tatcgctggt ttcgttgccg gtaaacaggg taatgaagtt
    37381 tttcttcggc attttgcact gctcaacgca gagcttcatg atcagacgtt cttgcgtacg
    37441 aacgcggtcc atcatgacgc gcatgctgtt gaccaggtag tcaaactgct tcggcaccag
    37501 gcggaactgt ttgaatactt cagacagttt caggatctct tcctgagcgg cagcgtgact
    37561 gcgacctttc gctttgatgg tgtcacgcgt tacaacgtac tgagcgcgta gttccgcaaa
    37621 tttttcgcga gccagttccg ggtcgatgct gttgtcatca tcggcgctgt cgtcatcgcc
    37681 atcttcttcg tcttcatctt cgtcatcgtc cagatcttcc tgggaaagct cagaaccgac
    37741 gtgagtggcg gtaggtgcca gatcttcttc tgcgttcggg tcaacaaagc cggtgatcag
    37801 atcggacaga cgcgcttctt ctgcttcaac acgatcgtac tgttccagca gataggtgat
    37861 cgcttccgga tattcagcaa cggagcattg aacctggttg atcccgtctt caatacgctt
    37921 agcgatgtca atttcgcctt cgcgggtcaa cagttcaacg gtgcccattt cacgcatgta
    37981 catgcgtacc gggtcagtcg tgcgcccgat ttcagattcc acgctggaaa gcacctgcgc
    38041 ggcggcttcg gcagcatctt cgtccgcggt gttttcagcc agcatcagat catcggcatc
    38101 cggtgcttct tccatcacct gaatgcccat gtcgttgatc atttggatga tgtcttcgat
    38161 ctgatctgaa tcgacgatat cttccggcag atggtcattg acctcggcat aggtcagata
    38221 gccttgctcc ttaccacggg tgacaagaag tttcagctgt gactgcgggt tttgctccat
    38281 aagacggtat ccacacttat ttcatgaggt tggtgttggg cgattgaccc gatgtccttc
    38341 agccgttaac gctgaaatcc aacggttgtt taccgccaac gataattacg agggcgtact
    38401 tatatatttg ccgctgcctc tcagtgcggc tgtcgggggc ttcccgatcg ctcttcggca
    38461 cttaagccgt taaatcactt tttcgccagc tccaggttta atgtccagag ctccaggcgt
    38521 tcttcgttgc ttaaaccatg cgtgcgctca cgagcgatta actcttcctg gcgcagttca
    38581 agcagcgaat caaacatatg gttgagtgag tcggtgaagg tttgctcagc aatattctta
    38641 tctgctatat cgtcccacat cgacagtttt tcaagggtgg cagcattatt tgtaccacga
    38701 tagtgctcta aaagttgccc ggtggtcaga cctggctggg agagacaagt gttgaccagt
    38761 tctctgaata agccaagtcc agggagctta ttttcatcca gattctcaag cggcgggacc
    38821 aacgtcgcta attctggatt ttgcaccagc aaccctataa gtatacgcat ggtcgtgcgt
    38881 tttagctgcg gaacagggcg agaaacgccg ctctctgccg cttttggcat taatcgttca
    38941 agctggctgt catcaagtat gcctaatttg ttgcctaatt cctgacgaag atatattcgc
    39001 agcgtttcgc ccggcacttg cgatatcaat ggcagtgcca gcgtactcaa acgtgcgcgc
    39061 ccgtcagggg tactcagatc aacttgcggc atcaggctgt taaacagaaa tgcggagagt
    39121 ggcatcgcct gctccatccg cgcttcaaac gcttctttac cttcttttcg taccagcgtg
    39181 tcagggtctt cgccatccgg caaaaacata aagcgtagct gacggccgtc tgtcatgtaa
    39241 ggcagcgccg tttccagcgc tcgccatgcg gcatcgcggc ctgcacggtc gccgtcataa
    39301 cagcaaatga cattgttggt cgcgcggaac aacagttgta tgtgatcggc ggtggttgac
    39361 gtacctaacg acgcaacggc gtaattaatg ccgtattgcg ccagcgccac cacgtccata
    39421 tagccttcga ccacaagcag acgattgggt tcagcgttat cctgctgcgc ttcataaaga
    39481 ccgtaaagct ggcggccttt atggaaaatg tctgtttccg gcgagttcag gtatttgggg
    39541 gtatcgttgc ccagcacgcg cccgccaaaa ccaatcaccc gaccgcgttt atcgcgaatg
    39601 gggaacatca cccgctcgcg gaaacgatcg taactgcgtc cctgatcgtt agtgaccaac
    39661 atccccgcat caatcaatga ctggcgattt tctggattgc cgccaaaccg cttcaggacg
    39721 ttgtcccagc cggggggcgc aaaaccaatc gcaaagcgag cgataacctc gtggcttaat
    39781 ccgcgttttt ccagatactg gcgcgcagac gtggcaacag gttgttgtaa agattgttgg
    39841 taaaacgtat tcagaccgtc catcaactga taaagcgttt gcctctgatg gcgctcgatc
    39901 tggctggggc cgctgcctgc ttcaaatggc acttcaagat tgtgcattgc tgccagctct
    39961 tcgaccgttt caacgaactc gagcttgtcg tagttcatca ggaagtcgat cgcgttgccg
    40021 tgcgcgccac atccaaagca gtggtaaaac tgtttctcac cgttaacggt gaatgacggg
    40081 gttttctcgt tgtggaatgg acaacacgcg tggaaattct tgccctgctt tttcagcttc
    40141 acacgggcat cgatcagatc gacgatgtca gtgcgtgcca gcagatcatt aatgaatacg
    40201 cgtgggattc gtccagccat aggccccgat ttttagcaat tcataaacga aaataagccg
    40261 cgcattcctt tcggaagcac ggccttacaa ctacaactcg gtctgatcgg agagcaacgc
    40321 tctcggggaa ttagtacaga cgagtgcggc gtgcgttttc gcgagccagt ttcttcgcgt
    40381 gacgtttcac tgcagaagct ttagcgcgct tacgttcggt agtcggtttt tcatagaact
    40441 cacgacgacg aacttccgcc agaacacctg ctttttcgca ggaacgcttg aagcgacgca
    40501 gagctacgtc gaacggctcg ttttcacgta ctttaattac cggcatgtgc ctctcacctt
    40561 tgattaattc ggtttgccgc tggcatcaac gccagcttat ttcaaaatgg tgcggaattt
    40621 tactgcaatt gctgctgctt tgtaaagcac cgcggccttt tttgaaaggg acttttataa
    40681 gggtgaggag tatacacgag gctttctcca ggggcgaaca aagttttaca tcaacccgca
    40741 ttggtcctac actgcgcggt aataaagcga ggtaaaacaa gtcatgcgtg tactgggtat
    40801 tgaaacttcc tgcgatgaaa ccggcatcgc catttacgac gatgaaaaag gtttgttagc
    40861 caaccaattg tatagtcagg tgaaattgca cgctgactac ggcggcgtcg tgcctgaact
    40921 ggcctcccgc gatcacgtgc gcaaaaccgt accgttgatc caggcggcgc taaaggagtc
    40981 tggtttaacg gcaaaagaca ttgatgctgt ggcctatacc gcaggccctg gattagtcgg
    41041 cgcactgctg gttggcgcaa ccgtggggcg ttctctggcg tttgcctgga acgttccggc
    41101 aatcccggta caccatatgg aagggcatct gttagcgccg atgctggaag ataacccgcc
    41161 ggaatttccg tttgtcgcgc tgctggtgtc cggcggtcat acgcagttaa tcagcgtgac
    41221 tggcattggt cagtacgagc tgctcggcga gtctatcgat gatgccgccg gtgaagcgtt
    41281 tgataaaacc gcgaagctgc tggggctgga ttatcctggc ggaccgttac tgtcgaaaat
    41341 ggcggctcag ggtactgccg ggcgctttgt tttcccgcgt ccgatgaccg accgtccggg
    41401 gctggatttc agcttttctg gtctgaaaac ctttgcggcg aacacgattc gtgacaacgg
    41461 caccgacgac cagacgcgtg ctgatatcgc ccgcgccttt gaagatgcgg tggtcgatac
    41521 gttgatgatt aagtgtaagc gagcgttgga tcagacgggc tttaagcgac tggtcatggc
    41581 gggcggcgtg agtgctaacc gcacgttacg ggcgaagctg gcggaaatga tgaaaaaacg
    41641 ccgcggcgaa gtgttctacg cgcgtccgga attttgtact gataacggcg cgatgatcgc
    41701 ctatgccgga atggtgcggt ttaaagcagg cgcgacggcg gatctcggcg ttagcgtgcg
    41761 tccgcgctgg ccactggcag agttaccggc tgcgtaaaac tatccggtgc cggagagcaa
    41821 tttccggcac cgtcctcact taaagcaaca ccacgggcat ccacagcaag ccggtaatca
    41881 ccagcaacac gaggaatatc agtccaaaaa tcgcccccag tcgccagtaa tccaccgttg
    41941 gcagataacc actaccgtag taaatcggac tgggtccggt ggcgtatggt gtaagaatgc
    42001 tccccagacc aattgccgcg ccaaccatca ggcagaacac cggcagcggg atttccggca
    42061 tcgccagcgc agcggcaatc atcatcggtg cgagggcgga ggtatacgcc gtggcgctgg
    42121 cgaaaaagta gcgcagcaga taaaacacca caatcaacgc caccatcacc atcgttggcg
    42181 aataaccgct taagctgcct gctaacagtt tgccaaacca gctaataaaa ccagtgttgt
    42241 tgagtccggt agcgagggtg ataagcgagg ccagccagaa gaaaacgttc cacgccgctt
    42301 tattactgac aatgtcgtcc caactgataa tgcgcagaag cagcatcagt gccaccacgc
    42361 tgtaaccgac catcgcggca tcgatataat caccgccgaa aatccacagc accagcgcgc
    42421 ctaccatcag ccccagcatc cgtttttcac gcgaacagag cgggcccatt gcctgcagtt
    42481 ccgtctctgc ccagcgcggc acctgatcgc cagacttcag taccggcggg tacagcacat
    42541 aagccagcca gggaaccagc agcaccagca aaatactgag cggcaacatc ccgaggaacc
    42601 agtcgcccca actcagcgtg gcgtgagatg cgcttttcat cagtccaatt aacagcaagt
    42661 ttggtgccat cgccgtcaag aaaatggcgc tggtcacgca gtcggcaaca atccccatcc
    42721 acatgatgta cgagccaatg ctgcgcgaac tgctgtcgtt tggttgtgat tgatagagcg
    42781 gtggcaggtt acggatgatg ggatagataa tccccgcacc acgcgccgag ttggacggtg
    42841 ttacaggtgc gaggatcagc tcggagaaca tcaccgcata gccgagaaac agcgtgcgat
    42901 gtcccatctt tttcaccaga aacagcgcaa tgcggcgtcc aagcccggtt ttttcatagc
    42961 ctgtgccaaa cataaaggcg gcgaaaatca gccagataac cgaattagaa aaaccggaaa
    43021 ccgcccacga gagggatttt gcagtgaatt taaagcctgg ctgggcgagc tgttcggggc
    43081 tgaacagcag ccagggagag agtatggcga tgatggagat acccaccatc gccaccacgg
    43141 cacccggcac gggttcgagg atcagtccaa cgatcacgcc agtaaaaacg gcaaagtaga
    43201 gccaggtatg gttctccagc cctgcgggaa ccggaagtag agcaataatg gcgatgaccg
    43261 ccagcggcgc aagatatcgc caccattcag tggaaggttt catcgtgtta tgcctgcgaa
    43321 aaaggagagg ggtgagcctc tccgggaggg ttatttgatg taatggacgt gctcgcagat
    43381 ctcttccacg atgggatcgc ggcgttcggc gaacagtttt ttgttttcgg ctatcaggtt
    43441 gttgccgtgg gtatcaatag agacaatcag cgggccgaac tctttgaccc ggcagaccca
    43501 cagtgactcc ggcatcccga gttctgtcca gtgcacttct tcaatctctt ctacctgggt
    43561 tgccgccagc accgcgcagc ctgccgggaa aatcacatgt agcgccttga atttctggca
    43621 gccttcttcg gtcagcggcc ccataccacc tttgccaacc accagtttca cgccggtctg
    43681 ctcgataaat tcacgttcaa aactttccat acgcatactg gttgtcgggc caacggaaac
    43741 catctcccat ttatcgccgt ttttgcgcac gatggggcca gcgtggaaaa tcgctttgcc
    43801 gttgagatcg taagggatcg gacgtttcag ttcgatcaag cggcggtgac aaacgtcacg
    43861 gcaggtcacc agcgtaccgg tcaggtagat cacatcgccg acgcgaatat cttgcagatc
    43921 ttcagctttg atcggggttg tcaggatctt tttcataacg cgctccgggt gtgagacaga
    43981 ttttcaaagg tgagatcggc atgaaccagc agtgtgccgc gacgatgcgc ccagcagcct
    44041 gtagaaacag caacgccgat ggttgacgga tggcgggcgg cagattcgat atgcacgccc
    44101 atcactgaac tgttgccagt cagcccttgt gggccaattc ccagacggtt gagtccttct
    44161 tccaggcgta gctccagctc tgccgctttt ggattgggat ggcggctgcc aatcgggcgc
    44221 aaaatggctt ttcgcgagag cacggcggcg gtttccaccg aggtggcgat gcccacgccc
    44281 accagtaccg gtggacaggc gtttacggcg agggtggaga tattttcgaa gacgaatttc
    44341 accacgcctt cgtagccttc tgacggcatt aacactttcg agcggccagg tagcgtgcag
    44401 ccgccgcctg ccatgtaaac ttcgatttcc gcatcgtcat tgtcggggat gatgtcccag
    44461 gtgacccacg gtacgccgct accggtattt ttgccggtgt ttacttcgtc aaaaatttct
    44521 accgcattgt gacgtagtgg cgctttgacg gttgcctctt ccacggcttg tttgagtatg
    44581 ctttgcagct cgccaagcag tgggaagcgg gaaccgactt taacgaagaa cataatctcc
    44641 ccggtgtcct gacaggcagg acgattcagg tcaatcgctt tttgcatgtt gtcgaacatc
    44701 gtatggtaga taattttccc catcgacgac gtttcggcat cctttagctg ttttagttta
    44761 tccaccacgt catcaggcat tcgggtagaa atcatggcgg taaagttagc gacaatctct
    44821 gtcaacttat tcactgcctg ttgcttatta ctttcgctca tcatggcaac tccagttttt
    44881 gtatttgtct ggaaaaattc accgccacaa actaccacta aaaatgtggt tttatatttt
    44941 ttgccaccgt gaatatgtaa ttaacagttc gtgaatcaac gcgaaaataa gaatgaatat
    45001 tgcgccagag cagggaatgg caatgttttg cgaaatacac tgcgcttatc acaactgcgg
    45061 ataattaacg atgctgaata gctggccttt agccaaagac ttgcaggtac tggtagaaat
    45121 cgtccacagc gggagtttta gtgctgcagc ggcgacgctt gggcagacgc ctgcatttgt
    45181 caccaagcgg atccaaatcc ttgaaaatac gctggcaaca acgctgctga accgctccgc
    45241 tcgcggcgtg gcgctgactg aaagtggcca acgttgttac gaacatgcgc tggaaatcct
    45301 cacccaatat cagcgactgg tcgatgacgt cacgcaaatc aaaacgcgcc cggaggggat
    45361 gattcgtatt ggttgcagtt ttggttttgg gcgcagccat attgcgccag ccattaccga
    45421 acttatgcgc aattatcctg agttacaggt gcattttgaa ttgttcgatc ggcaaattga
    45481 tttagtgcag gataatattg atctggatat tcgtattaat gacgaaattc ctgattatta
    45541 tattgcgcat ctgttaacga aaaataaaag aatattatgc gcagcacctg agtatctgca
    45601 aaaatatccg cagccacaat cattacagga attaagtcgt catgactgtc tggtgaccaa
    45661 agagcgcgat atgacccacg gaatatggga gttggggaac ggtcaggaga aaaaatcggt
    45721 gaaggttagc gggcatcttt cctccaatag cggcgagata gtgttgcaat gggcgctgga
    45781 gggcaaaggg attatgctgc gttccgagtg ggatgtgttg ccgtttctgg agagtggcaa
    45841 actggtgcaa gtattgccgg agtatgcaca aagcgccaat atctgggctg tttaccggga
    45901 gccgctctat cgcagcatga aattacgtgt ctgcgtggaa tttctggcgg catggtgcca
    45961 gcaacggctg ggcaagcccg atgaaggcta tcaggtcatg tagatccacc agaaatcact
    46021 cgggatcctt ttcgcgcttt cttttgaatt tcgtccagat ttttgtctcc tgacgacgcc
    46081 acagacgctg gatgttgtca tgatgacgca gcaggatcag gcaagagagc atcgaaaccg
    46141 ggaaggtgaa ttgtggctta aaccaccata cataaaacgg agcaatcagt gcactgacaa
    46201 tcgctcccag cgacgagtat ccgctcaata gcacggtcag taaccaggtt cccgccatta
    46261 ctccggtgag atcccagcca atgggtgcga tggcaccaaa agcggtagca acgccttttc
    46321 ctcctttaaa tccgaagaaa acgggccaga tgtgtccaag acaggcggcg atggcaatta
    46381 agcctagcca gaaggggctg acacctaatt catacgcgcc ccagacgggc aacattcctt
    46441 tcagaacgtc gaaaatcagt actgctacgg ctgctccctt gccaccgata cgtaacacat
    46501 tggttgcgcc tggattgccg gagccgctgg ttcgcggatc gggcagccca cacaagcggc
    46561 aaaccagaat ggcactggaa atggagccgc agaggtacgc gatgaggatc attccaggcg
    46621 cgattgcact cataagctgt tccgttttga aaattcgtgt tcaacgatga atctgtggat
    46681 aatacgcaca tttcgccgga agtggtatcc ggttagccaa aaagcaggca ggacgtgatg
    46741 gatattgtat ttatagagca actttcggta atcaccacta ttggtgttta cgactgggaa
    46801 cagaccatcg aacagaagtt agtgttcgat atcgaaatgg catgggataa ccgtaaagcg
    46861 gcgaaaagtg atgatgtggc ggattgcctc agttacgctg acattgcaga aacggtggtc
    46921 agccacgtcg agggggcgcg ttttgcgctg gtggaacgcg tggctgaaga ggtggcggag
    46981 ctgctgttag cacgctttaa ctcgccgtgg gtgcgtatca aactcagcaa gccaggcgca
    47041 gtggcgcggg cggcgaatgt tggcgtaatc attgagcgtg gcaataatct gaaagaaaat
    47101 aattaatttt acagctgtta aaccaaacgg ttataacctg gtcatacgca gtagttcgga
    47161 caagcggtac attttaataa tttaggggtt tattgatgag cgatatgcac tcgctgctga
    47221 tagcggcaat attgggtgtg gtcgaaggat tgacagaatt tctgccggta tccagcacgg
    47281 gccatatgat tattgtcggt cacttgttgg ggttcgaggg cgacacggcg aaaacctttg
    47341 aagttgtgat ccagttagga tcaattctgg cggtagtagt gatgttctgg cggcgtctgt
    47401 ttggcctgat tggcatccac tttggccgcc cgttgcagca cgaaggagaa agcaaaggtc
    47461 gtttaacgct gatccacatt ttgctgggga tgattccggc ggtagtactg gggctgttgt
    47521 tccacgacac gattaagtca ttgtttaacc cgataaatgt gatgtatgca ctggtcgttg
    47581 gcggtttgtt gctgattgcc gccgaatgcc tgaagccgaa agagccgcgt gcgccgggtc
    47641 ttgatgatat gacctatcgt caggcgttta tgattggctg tttccagtgt ctggcgctgt
    47701 ggccgggttt ctcccgttcc ggggccacca tttcaggtgg gatgctgatg ggcgtgagcc
    47761 gttacgctgc ttccgagttc tcgttcctgc tggcggtacc gatgatgatg ggcgcaacgg
    47821 cgctcgatct ctacaaaagc tggggcttcc tgacaaccgg cgatatcccg atgtttgccg
    47881 ttgggtttat caccgctttt gtggtggcgc tgatagcgat taaaaccttc ctgcaattga
    47941 ttaagcgcat ttcgtttatc ccgttcgcca tttatcgctt tattgtggcg gctgcggtgt
    48001 acgtcgtgtt cttttgattg agaaaaggtt cgggagcagg cctggttttc tccctcgctc
    48061 tgtagggaga gggccggggt gggggtgatt gttcgcaccg gtgctggtct gttcccctca
    48121 ccctaaccct ctccccgaag gggcgagggg atggttcgtg ttaacgttta gttttatact
    48181 cagtcaggct ttgggcaacg ttgttccttc caactggcta ccgccgcaat ccgccgtcgg
    48241 gtcagctcct cgcgaatctc cacaccttta aatcccgctt caacgaccgc ttttgtcggc
    48301 actgactgcg ccacttccca ggcttcgcgc aaccaacggc cttgcgggta gtccgctgat
    48361 tcaaaaccgg ttctgccgcg tacgtcagcc tcgctggtca gcgccagttg ctcgacacgc
    48421 tgcggtttac gccaggcatc gatggaatca aataatttga cgatggtttt tgggttcagc
    48481 attgggaagg tgtggatgag atcgtgaaac tcagccacca gtctggctaa atcgcgaatt
    48541 tcatttggca cgcgcagacg ctggcatagt tgttccacta acttaacacc cgccgggcca
    48601 tgcccatgat gacgcggcca aagttctggc ggcgtcagtc ctttaccgag atcgtggcat
    48661 aaagttgcga aacggacatc aacctgcgga ctcagcatcg ccgccattga gagcgtcatt
    48721 aaggtatgaa tacccgtgtc gatttccgga tgccacctgg caggggccgg aacgccaaac
    48781 agggcgtcaa tttccgggaa taaaacgcga agtgcaccgc aatcgcgcag tacctggaag
    48841 aacacctggg gattgcgggt ggtgagggcg ctttccgtct ctttccatac ccgttcaggc
    48901 gtcaggtgtt ccagttcacc cgcatgggtc atctcgcgca tcagcgtcag agtttcatcg
    48961 gcaatacgaa aaccgaggtg ggcataacgc gcagcaaaac gcgccacgcg cagaacgcgt
    49021 aacggatctt cgccaaaagc gggggaaaca tggcgcaaca gacgattttg cagatcgccc
    49081 agaccgttgt acgggtcgat aatctcgccg ttatcgtcct gagccagcgc attaatggtc
    49141 agatcgcgac gcttaagatc atcttccagc gtgacatccg gtgcggcata gcaagtaaaa
    49201 ccggtgtaac cggaaccgga tttccgttcg gtacgtgcca gcgcatactc ttcatgcgtt
    49261 agcggatgca gaaacacagg aaaatcgcgg cctacctgct ggtagcccgc gtcgagcatc
    49321 tcctgtggcg tactgccgac caccacccaa tctctgtctt tgaccggtag ccctaacaat
    49381 gcatcccgaa cagcaccacc gaccagataa atcttcacgc catcatccct tcgtcaaata
    49441 cacaattcct aataataaga cagtgcaacg aagaaggcga tttagttcat ccagcgatct
    49501 ttgcgtttgc gcgttgggat caggtgtggc agtaccagac cgagcagcaa gccaagcccc
    49561 agcacgccac cgccatacat aaaccattgc atgatgatgg tgcgctgttt gtcatccaac
    49621 tgtacgctgg cggcatcgac ctttttctgc gcgacaatca gctcgttttt caatttctga
    49681 ttttcttctt ttaatccgtt gatcacgctg tcgctctgcg ccactttttg ctgcatttct
    49741 gcggtgcgct gattccaggt gttatcgata ttggtgagtt tatcggtcag ggttttgacc
    49801 tgattttcca gatctggcac acgggagcgc aggcttggct cagtgctaag ttgtttcaac
    49861 gggatccagg cggtacggcc agagctgtct ttcacctggg cataattggt gttggcgtca
    49921 gtttgtaata aggtcacttc ctcgccggcg ttaaccgtgc ccacgaggcg ataatgatct
    49981 cccggaccgc tacggaccca ggtattcagt tcatcagaaa catagcgcgt ttcttcagcg
    50041 tgtgagacgg cagtggcgct aagtgcgagt aaagttaatc cgatcaggcg taattttggc
    50101 atcaggctgt cgttattgtc atggaaagtg gaacgatagt agtggcatca gtgtcgctac
    50161 gcaaagcatt cgacatcaat cggaatcctc tgtgtcaggt cttgccactg cttcaaactt
    50221 ttacgcccgt caaattgccc gttgcatggc aatttggcgc aaaatactat ctactgacaa
    50281 aaaagatcgc attaagttcg ccgttcatcg tcacttatgt gacataaccg ataagtaaag
    50341 gccatggctc aggaaatcga attaaagttt attgttaatc acagtgccgt tgaggcgttg
    50401 cgtgaccatc tcaatacgct gggcggcgag caccatgacc ccgtgcagtt gctgaatatt
    50461 tactacgaaa cgccggataa ctggctgcgt gggcacgata tgggcttacg tattcgtggc
    50521 gaaaacggtc gctatgagat gaccatgaaa gttgcaggaa gagtgacagg cggcttacac
    50581 cagcgcccgg aatataacgt ggcgttgagc gaaccgacgc tcgacctggc gcagttaccg
    50641 acggaagtct ggccgaacgg cgaattgccc gccgatctcg cttcccgcgt acaaccgctg
    50701 ttcagcaccg atttttatcg cgaaaaatgg ctggtggcgg tcgatgacag ccgaattgaa
    50761 atcgctctcg atcaggggga ggtgaaagcg ggggaatttg ctgaacctat ctgtgagctg
    50821 gaactggaac tgcttagcgg cgacacgcgc gcggtgctga aactggcgaa ccaactggta
    50881 tcgcaaaccg gattacgcca gggcagcctg agcaaagcgg cgcgtggcta tcatctggcg
    50941 cagggcaatc cggcgcgtga aatcaaaccg accaccattt tgcatgttgc ggcaaaagcc
    51001 gatgtggaac aggggctgga agcggcgttc gagctggcgt tagcgcaatg gcagtatcat
    51061 gaagaactgt gggtacgcgg caacgatgcg gcgaaagagc aggtgctggc agccattggc
    51121 cttgttcgcc atgcactgat gctgttcggt ggcattgtgc cgcgtaaagc gagcactcac
    51181 ttacgtgatt tgctgactca atgcgaagcg accatcgctt ctgcggtttc tgccgtgacg
    51241 gcggtctact ctaccgaaac ggcaatggca aagctggcgt tgaccgaatg gttggtaagc
    51301 aaagcctggc agccgttttt agatgccaaa gcgcagggta aaatcagcga ttccttcaaa
    51361 cgctttgccg atatccatct ttcccgccat gccgctgaac tgaaaagcgt tttctgccag
    51421 ccgttaggcg atcgctacca tgatcagttg ccacgcctga cgcgtgatat tgactcaata
    51481 ctgctgctgg caggttacta tgatcctgtc gtcgcgcaag cctggctgga gaactggcag
    51541 gggctgcgtc acgctattgc gaccgggcaa cgcattgaaa ttgaacattt ccgtaatgag
    51601 gcaaacaatc aggaaccgtt ctggttgcac agcggaaaac gttaatcagg caaggataaa
    51661 atcgcttatg aagccgctct cttcaccgtt acagcagtac tggcagaccg ttgttgagcg
    51721 gctgccagag cctttagccg aggaatcact tagcgcacag gcgaagtcag tacttacttt
    51781 tagtgatttt gtgcaggaca gcgtgattgt gcatccagag tggctgacgg aactggaaag
    51841 ccaaccgccg caggccgacg aatggcagca ttacgcggca tggttgcagg aggcgctcag
    51901 taatgtgagt gacgaagccg ggttaatgcg cgagctgcgg ctattccggc ggcgcattat
    51961 ggtgcgcatc gcctgggcgc aaacgctggc tttggttact gaagagagca tattgcagca
    52021 gctcagccat ctggcggaga cgctgattgt cgccgcgcgt gactggctgt atgacgcttg
    52081 ctgccgcgag tggggaacgc cgtgcaatgc gcagggcgaa gcgcaaccgc tgctgatttt
    52141 aggcatgggt aagctgggcg gtggggaact gaatttctcc tcagatatcg atctgatttt
    52201 tgcctggccg gaacatggtt gtacgcaggg cggacgccgg gaactggata acgcgcagtt
    52261 ttttacccgc atggggcagc ggctgattaa agtgttggat caaccaacgc aggatggctt
    52321 cgtctatcgc gtggatatgc ggctgcgtcc gtttggcgaa agtggcccgc tggtgctgag
    52381 ctttgccgcg ttggaggatt attaccagga gcaggggcgc gactgggagc gttacgcgat
    52441 ggtcaaggcg cggattatgg gcgatagcga aggcgtctat gctaacgagt tgcgtgcgat
    52501 gctgcgcccg tttgttttcc gtcgttacat cgatttcagc gtgattcagt cgctgcgcaa
    52561 catgaaaggg atgattgccc gtgaagtgcg tcgacgtggt ttgaccgaca atatcaaact
    52621 cggcgcaggc ggcattcgcg aaattgaatt tatcgttcag gtgttccagc tcattcgcgg
    52681 tgggcgcgaa ccgtcgctgc agtcgcgctc tttactgcca acgctcagcg tcattgccgc
    52741 gctgcatctg ctttctgaaa acgatgctga acaattgcga gtggcgtatc tgttcctgcg
    52801 gcgtctggaa aacctgctgc aaagcattaa cgacgaacaa acccagacgc ttccttctga
    52861 tgagcttaac cgtgcgcggc tggcgtgggc gatggacttt gctgactggc cgcaactgac
    52921 cggggcgctg accgcacata tgaccaatgt gcgccgggtg tttaatgaat tgattggcga
    52981 cgacgaaagt gaaactcagg aagagtcgct gtcggaacag tggcgtgagc tgtggcagga
    53041 tgcgttgcag gaagatgaca ctacgccagt gctggcgcat cttagcgagg atgatcgcaa
    53101 acaggtgcta acgctgattg ccgatttccg caaagagctg gataagcgca ccatcgggcc
    53161 gcgaggacgt caggtgctcg accatctgat gccgcatctg ctaagtgatg tctgtgcgcg
    53221 tgaagacgct gccgttacgc tgtcgcgcat taccgccttg ctggtgggga ttgttacccg
    53281 caccacctat ttagaattgc tcagtgaatt ccccgcggcg cttaaacatt tgatttctct
    53341 gtgtgccgcg tcgccgatga ttgccagcca actggcgcgt tatccattat tgctggatga
    53401 attgctcgat ccaaacaccc tttaccagcc gacggcgacc gatgcctacc gcgatgagtt
    53461 gcgccagtat ttgctgcgcg tgccggaaga tgacgaagag caacagcttg aggcgctgcg
    53521 tcagttcaaa caggcacagc tgttacgcat cgccgcagcg gatatcgctg gtacgctacc
    53581 ggtgatgaaa gtgagcgatc acttaacctg gctggcagag gctatgatag atgccgtcgt
    53641 tcagcaggcg tgggttcaaa tggtggcccg ctacggtaag ccaaatcacc tcaacgatcg
    53701 cgaaggccgt ggttttgcgg tggtcggcta cggcaagctg ggcggctggg agttaggcta
    53761 cagttccgat cttgacctga tcttcctcca tgattgccca atggatgcga tgactgacgg
    53821 cgagcgggaa atcgacgggc gacagtttta tctgcgtctg gcgcaacgca ttatgcatct
    53881 gttcagtacg cgtacctctt ccggcatttt gtatgaagtg gatgcacggc tacgcccgtc
    53941 cggggccgcg ggaatgctgg tgacatccgc agaagcattt gccgattatc agaaaaacga
    54001 ggcctggacg tgggaacatc aggcgctggt gcgtgcgcgg gtagtgtacg gcgatccgca
    54061 gctcaccgcg cactttgacg cggtgcgtcg cgagattatg acgctgccgc gtgaaggtaa
    54121 aactctgcaa acggaagtgc gggaaatgcg cgagaaaatg cgcgctcatc tcggcaataa
    54181 acatcgcgat cgctttgata tcaaagctga tgaaggcgga attaccgata tcgaatttat
    54241 tacccaatat ctggtgttgc gctacgctca tgaaaaaccg aagttaacgc gctggtcaga
    54301 caacgtgcgc attctggaac tactggcgca aaacgacatt atggaagagc aggaagcgat
    54361 ggcgctgacc cgtgcgtata ctacgctgcg cgatgaactt catcatctgg cattacagga
    54421 attgccgggc catgtgccgg aggattgctt caccgcagag cgtgaactgg tgcgggcaag
    54481 ctggcagaag tggctggtgg aagaatgaag tatggtatta tcgcgcgcaa attttgaatc
    54541 tctcaggaga caggaatgaa agtaacgctg ccagagtttg aacgtgcagg agtgatggtg
    54601 gttggtgatg tgatgctgga tcgttactgg tacggtccca ccagccgtat ctcgccggaa
    54661 gcgccggtgc ccgtggttaa agtcaatacc atcgaagaac gtccgggcgg cgcggctaac
    54721 gtggcaatga acatcgcttc tctcggtgcc aatgctcgcc tggtcgggct gacgggcatt
    54781 gacgatgcgg cgcgtgcgct gagtaaatct ctggccgacg tcaacgtcaa atgcgacttc
    54841 gtttctgtac cgacgcatcc gactatcacc aaattacggg tactttcccg caaccaacag
    54901 ctgatccgcc tggattttga agaaggtttc gaaggtgttg atccgcagcc gctgcacgag
    54961 cggattaatc aggcgctgag ttcgattggc gcgctggtgc tttctgacta cgccaaaggt
    55021 gcgctggcaa gcgtacagca gatgatccaa ctggcgcgta aagcgggcgt tccggtgctg
    55081 attgatccaa aaggtaccga ttttgagcgc taccgcggcg ctacgctgct gacaccgaat
    55141 ctctcggaat ttgaagctgt tgtcggtaaa tgtaagaccg aagaagagat tgttgagcgc
    55201 ggcatgaaac tgattgccga ttacgaactc tcggctctgt tagtgacccg ttccgaacag
    55261 ggtatgtcgc tgctgcaacc gggtaaagcg ccgctgcata tgccaaccca ggcgcaggaa
    55321 gtgtatgacg ttaccggtgc gggcgacacg gtgattggcg tcctggcggc aacgctggca
    55381 gcgggtaatt cgctggaaga agcctgcttc tttgccaatg cggcggctgg cgtggtggtc
    55441 ggcaaactgg gaacctccac ggtttcgccg atcgagctgg aaaatgcagt gcgtgggcgc
    55501 gcagataccg gctttggtgt gatgaccgaa gaggaactga agcttgctgt agcggcagcg
    55561 cgtaaacgcg gcgaaaaggt ggtaatgacc aacggtgtct ttgacatcct gcacgccggg
    55621 cacgtctcct atctggcaaa tgctcgcaaa ctgggtgacc gtctgattgt cgctgtgaac
    55681 agcgatgcct ccaccaaacg gctgaaaggg gattcccgcc ccgttaaccc gctcgaacag
    55741 cgtatgattg tgctgggcgc actggaagcg gtcgactggg tggtgtcgtt tgaagaagac
    55801 acgccgcagc gcttgatcgc cgggatcctg ccagatttac tggtgaaggg cggcgactat
    55861 aaaccagaag agatcgccgg gagtaaagaa gtctgggcca acggcggcga agtgctggtg
    55921 ctcaacttcg aagacggttg ctcgaccact aacatcatca agaagatcca acaggataaa
    55981 aaaggctaac cggaaagcgg ttcacagatc tcgcttcgta gcaggcaggc ctattgacgc
    56041 tgttgttttt tcaacctaaa gtaaaggaac aagggtaagg gaggatttct cccccctctg
    56101 atcagttgtt agtaagtcgg gaaacttaac agtaacaaca caaccagtat gatgacgaac
    56161 ttcatcataa ccctttcctt ctataaggcc ccttcctcgg gaggggcttt cccgtttcag
    56221 ccccttgctg actccccgat tgttgatgag cgccggaccc cgcgcaacgc tttccctgaa
    56281 gcagttttgc cttccccgag cggtcacgca aaacgctgca acgtcctgag catttgcacc
    56341 ttttagcgga acgcagatat aaaaaatccc cggctttttg ggccagggat atgtgttaat
    56401 gaaaccttct tgacgctgat tttttttcaa cctaaagtaa aggaacaagg gtgagggagg
    56461 atttctcccc cctctgattg gctgttaata agctgcgaaa cttacgatta acaacacaat
    56521 cagtatgatg acgagcttca tcataaccct ttccttctgt aaggcccctt cctagggagg
    56581 ggctttcccg tttcagccct ttgctgactc cccgattgtt gatgagcgcc ggacctcgcg
    56641 caacgctttc ccagaggcag ttttgccttc cccgagcggt cacgcaaaac gttgcaacga
    56701 cctgatcatt tgcagttttt agcggaacgc agatataaaa aatccccggc tttttgtgac
    56761 agggacgttc atagctgaaa atttattcta cgttttcggc gacgggagtt gttgaggtta
    56821 agggtgaagt caatgccgcc agtgagccgc ctgaaacgcc aatttcattg agcaaggagt
    56881 caatcagcgg tgcctgtgtg cggtaagaga gagcggctga taatgcttgt tccgccaggt
    56941 ttccgccccc cacattaccc gtgtttgcat cacccgcagc gctgccacga ttcagaccat
    57001 cgacctgaat aatcttgata ccgtcaatag atttcatcgg ctcaacggat ttttctatta
    57061 ccgcaggtag cgcctgcaac aaggccagtt tgaatttaag gctggtttgt tcatcagaaa
    57121 gtacgttgat agcatcgttc agcgcacgtt gtgcttctgc ttccgccaga ccctttttac
    57181 gtgtagcttc ggctaactca acgatagccg ccgcctgcat ctctgcggct tctttttctg
    57241 ctttcgcccg cacggtcagt tcaaccgctt tggtttctgc atcctgcgcg gcagcgatta
    57301 gggcaacttg tttagcacga tcggcttctg cggtctggcg agtggtttct acgttttgtt
    57361 gcgcacttac tgcttctgcg agtgcaaggt tagcacgcgc ttctgcctgg gactgttgtt
    57421 ccgatttggc ggcaatagcg atcgatttcg tctggttggc gatttcggtg acctgctgtt
    57481 gttcgatctc tttaatacga acttcacgtt cagcctcaac ctttcttgag cggacggcct
    57541 gctcgcgctc gatctccgtt tcctgaattt gtcgttcagc cagaattcgc gtctactctg
    57601 cttcacgacg acgttcagct tcaaaagccg caatacgtgc attctgttcg gcagtacggg
    57661 ttttaacctg ctgctcctgc tcaagcgtca taaacgcttc ttgttgttca atctccaact
    57721 tgcgcgaaag tgcatcacgg tttttctcac gcaccgcaac ttctacatcc tgttcaactt
    57781 cgttacgttc gcgacgacgg cgctccgtct cctgagtcag tttggttaaa ccttcggcgt
    57841 caaaggcatt gttcggattg aaatgttctt tcgaggtctg gttaaagttg gtaagtgaaa
    57901 cgctttccag ctcaagaccg tttttcgaca ggtcttctgc gacagtattt tgtactccct
    57961 gcacgaagtt ctcgcgggta tcctgtaact catgcatggt catttgtgca gctgttgcac
    58021 ggagggcatc gacaaattta tcttcaacca acatacgtaa gtcttctggt gatagcgtgc
    58081 gttgccccaa cgtctgcgcc gcagtggcga tgccttctac agacggtttt acccgtacaa
    58141 agaaagcgac tactacatcg acacgcatac gatctttcgt aatcaggcta tcaatggttg
    58201 agcggctgac ttccagcttc agagtattca tattgatggg gattatttca tgaaagatcg
    58261 gcatcacgat tgcgccaccg ctcatcacca ctttttgccc acctaaacca gtacgaacaa
    58321 aagcttgctc tgccgaagcg cgacgataga gcctggcgaa aataattcca ataataaaca
    58381 gaatgcatac ggcaataatc gcggtaaaca tccaggaggg cacagaatta acaatatcat
    58441 ccattattaa atttcctttt gattaataaa ttacaatatt tgtggccagg gattgttttc
    58501 cgccaaatat cgcgttgcag aaagtcggca aataattaac actttgtctc ctttagtgaa
    58561 aaattttcct tcttcaggct ccagtagtaa ataatgtatt tggccaaatt gatcggtgag
    58621 ttttccttcg cagggattac ctgaagtggc ttgatgaccg gtaattaatg ccatgctacc
    58681 aatatattct tcttctgtaa tagctgaact atggtcgcgt ggaatccagg gcgcaactat
    58741 tttaccggta taatgcaccg caataatcgt aaacagtaaa ctgacaggaa ccacgaacag
    58801 gttcgacagt ggtgattgcc agaccatgat gcaggcatgc tgcaacagga tgccaataag
    58861 accaaaaaaa ccagccagca agcagagaac gacgagagct ggcaatcttc cgatattgag
    58921 ataatgaagt gcctggctaa tatgacctgt ggttatggaa tcataatggt caagatgtgc
    58981 atcaagagca ccggagagca tgtgaccgca gataagcgct aaaatttcta aaaggccgat
    59041 caacagtaca aaggaaatag cgaaaagata aggggtatta tagtcggcga ataaaatcat
    59101 atgtcactcc aatgtccatt tagtgacgca atatttactt tgggatagtt tccttgctta
    59161 catttatctt tgttaataat aacacatgtt agagaagttc tggaagagaa agttttttaa
    59221 tatggttttt tatttttcac taaaaagtgt gatcggggac aatatattta cgcacgttat
    59281 gtttaaaggc actacactga ttggggaata ctgaaatcag aaaaagcata tggccttaaa
    59341 aggtatgcga taaccggagg atgtgcttat ggatcatagt cttaattctt taaataattt
    59401 cgatttcctg gcgcgtagtt ttgccagaat gcacgcagaa ggtcgcccgg tcgatattct
    59461 tgccgttact ggtaacatgg atgaagaaca tagaacctgg ttttgcgcac gttatgcctg
    59521 gtattgtcaa cagatgatgc aggcaagaga gctggagtta gagcactgat ataacgggcc
    59581 tgatggcccg ttttagtgtt tgtattagat gctagttata ggtgattttg aggatgacgt
    59641 ctttactaaa cggtccctca acaacagtct ctgccggatt cgcactaagt tctgccctga
    59701 atggtgcggt gaccgaaggt gcactagacg atgaaaaaga agcaaattgt gagaagcggt
    59761 taaattccgt tgctgttcca tctttatgaa atatcttcaa tagcagtcca ttacccatat
    59821 ccagatggct ataatcactc aaaatatccg tagtgaaaaa gctccctaaa atattgaatt
    59881 gctcagtaca ggccgcacca ggatcttttg tcatgactac actaaacgtc tcactaacgc
    59941 tggtattttt aattgttcgg gaatttattt taccaaagtc aacaacgcca tcagctggtt
    60001 ccagtgcgac attaacaaag cagtcaagga catgaatatt atttaatcca cgaatcctga
    60061 aggtcaaatt tttgtctact tctatagctc ctgcgccact cataccttta aactctagag
    60121 cgatatattc atcaggtaac accaggtttt tattaactct attggcattt tttgctaatt
    60181 taatatagag ttgtacgcga gctggaaagg ttactgataa ttgaggctca ggacctccac
    60241 tacagacagc ggggtttttt atagtgatta ttaaaggtac ccgctccttt tatatcaatg
    60301 cattctggtg ctgcgatagc attaggggca gtcgaatcat aatcaactcc attgtatgtt
    60361 acgccaaaaa tataggagtt ctgattataa aagtcctgac cgcctggtaa aaacatcatt
    60421 atattagtcc aggctgatgg gtattcttct ccaggtgatg gggcaggtaa tgcattatta
    60481 cagaagacat tcataaaata ctcactgctt tgccagatct tatcaccttt tttaccatta
    60541 ctggggatag aaattcttgg tacgtaacca atgactacgg tatttccccc ttcatgttca
    60601 gcatagcagt ccattgccca tacaggtaat gcactaaaac ctataataaa tataattaca
    60661 attaataagt agcgcatatt atttatctcg tttggctacg gaaacattac aagagttatt
    60721 aaccgtcggg cagtttattt cataaaaact taatccacca aaatcattga tataaccaac
    60781 ctgaaaagct gacggaacat ttttaaactg cgtatctgca tggctataag gggttaccat
    60841 taaactttgc gtttgtggca acattgtgat tccgtttgtt ccgatataac ccagcgtaac
    60901 gtgataaggc atcttattgt taaagcggat cgaaccatca tttctacgga tgatttcaac
    60961 tttttcgtaa gacattaccc catcatcttc taattcaata gcttttggtc gccagaatag
    61021 tttcaatgcg tgctgcatag tgacttgcat cacatttttt ttatctgttt ttggcggtat
    61081 ttctcgcacg ttaaaataga agagcgtttc tctgtcatta ggcagttgag caattttttc
    61141 ctgacttatc attcttattt gaatttgctc actgggattt aaacgtacca tagggggaac
    61201 cgtgacaata aagtcacgtg atttattgcc tttcgtatct tctatccatg tttgcgccag
    61261 atagggtaga tttttgcttt gattagtaag ttttaatgct tcccctttat ctgactcatt
    61321 cattatgata cgggtacggt ccgggatgat ggcaccccag gcaatgctgc tcatgcaggc
    61381 gagtaatact cctgctgcaa tgagattctt tgtgtttaag tagcgcatta gtttcatcct
    61441 gaattaattt tggctaatac acggtaaaat aaccgttccg gtggttaagt tgctgctatt
    61501 gggtggttga ataaaacagg atttatcacc ccagcgtaaa gtaagtttgc tgtcatcctg
    61561 aatgccgttg aggtatacaa agccgccatc agcaaccagt cctaattctt tatggctggt
    61621 tttatctttt acgctaatcc ccagtggtgg gtgactacca tctgccaggc gaataacccc
    61681 catgatttga tagcccttgc gggtatcaag tttggcgtag ccaatagcac cttcggttaa
    61741 ggtggtggtg ataaccgagt tttcgatatc cacattttct ggaagatttc gggtatcaac
    61801 gcttaatgat gttgttatgt aactgctgac tgatggaata actccgatgc cataccgatt
    61861 agttaccgcg cgtttattgt tcaacggaat atcttctgca ccatctgcgt cgatcataaa
    61921 acgcgagtca gcactaccac tatagtcatg gaaagcggct ccgtgacgag tcgccgtgaa
    61981 tgaaccattc caactggcat tcagagaata ataactgtct cgctgtgatg tcccggagag
    62041 atagagacga ccgttctcgg tattatgctg ataactggcg cgtagtgacg ctttcatatc
    62101 tttgaattca tcattgtcgc ctgaagcaga aacgctccag gaattatttc gatcggaaga
    62161 gtcataccac gaagcagtat gcgaaatctt gttatcctga tttcgttgca taccatagct
    62221 caatgttcta ctggttcccc agggaatcga aatatttagg tacagttgat cctggttgtc
    62281 ttcttcccag tttattcggc taaatgttag agaagtggag acgtttttta atggcccgat
    62341 atcaaaatcg cggctaagtg ataatgaata attcacatta ctgctggcgt cccagtaagt
    62401 attacgagcc aaacttacgt atgtatttaa ccttaatgat tcaagatact ggttaaacgt
    62461 gacaatataa ctctcttttt cattctgata attcgtgtaa tggttagtgt cattgatata
    62521 ttcattcatt gtcacaaagt ttttatcaga gaaacggtaa ccagcgaaag tgagctggct
    62581 accggtagat tcaaaacgtt ttgaatagtt agcgcggtag ctataacccg tttctttatc
    62641 ctgattatgc aactgagcat cagatcgtgt gacatcaaaa gataatgaac ccaatgagtt
    62701 aaggttaaag ccaacgccgg cagccagaga ttgataatca cggtttgtta aaactgaacc
    62761 accatacagg gatacattgt tcagccaacc ccatgagaat tcaccagtcc agaagaaggg
    62821 ttgttgtaag gaatcaccac caacgctggt cggtttaccc atagcagtgg tgtagcgaat
    62881 ctgcccttta cgcgtcagat aaggaatact attagatgca acttgccatt gcgtgacttg
    62941 cccgtcctct tcttcaacct tgacgtccag ttgcccctgt aatgtattga ataaattagt
    63001 aatagcaaat gggccaggag gaacattttc ttgataaata atacggttgt tctgtgagac
    63061 agtgaccttt gcattcgttt gtgcaacacc cgtgatttgc ggtgcgtaac cacgtagatc
    63121 aggcggcaac atattctcat cgcttttcaa cgaaacgcca gaaaaatgga aagagtcgaa
    63181 aatagaggat tcagtatcgt attgacctat agttagtttt gcattaattg ctgggattgg
    63241 gcgaaacaaa taaatacgcg gaaaatccag gttgttcgcc tggcttttgc cattgttaaa
    63301 tgactgatcg tactggtaat cagtacgcag gcgccaggcc ccaagattaa ccccagcctg
    63361 cccataggaa cttatatttt gtgaattatc gccctgatgt ggtgcataat gagaggcata
    63421 taaattgtag tcaagaaatg ccccagcaat accatgatcc caaagttcag gaggcgtcca
    63481 gtttgcatct ttgtatttta accatgcctg aggtgcagat atggataatt gcattttccc
    63541 tttatcgaga taagttgtaa tttgtttttc tttttcgata gggtaacaac catcgatcag
    63601 gttttgctga gcctcttcaa taatctcatc agtaaagcct aataatgtta attgctctgg
    63661 agtcagacaa actgcagttt ggttatcaat taatacccat tgcaggtcca gatggattgg
    63721 cgtcctttta ttattaataa gagcgtttaa tgagtattta cctacgggta attggccatc
    63781 agtttcaaat tgggaaaggt taacgttttc acga
//
LOCUS       DAZFWL020000013        62879 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFWL020000013 DAZFWL020000000
VERSION     DAZFWL020000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 62879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.013"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(64..1308)
                     /gene="mtr"
                     /locus_tag="ACHKNP_RS04505"
                     /old_locus_tag="pgaptmp_000901"
     CDS             complement(64..1308)
                     /gene="mtr"
                     /locus_tag="ACHKNP_RS04505"
                     /old_locus_tag="pgaptmp_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HGU4703116.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1462..3351)
                     /gene="deaD"
                     /locus_tag="ACHKNP_RS04510"
                     /old_locus_tag="pgaptmp_000902"
     CDS             complement(1462..3351)
                     /gene="deaD"
                     /locus_tag="ACHKNP_RS04510"
                     /old_locus_tag="pgaptmp_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HGU4703117.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3344..3424)
                     /gene="yrbN"
                     /locus_tag="ACHKNP_RS04515"
                     /old_locus_tag="pgaptmp_000903"
     CDS             complement(3344..3424)
                     /gene="yrbN"
                     /locus_tag="ACHKNP_RS04515"
                     /old_locus_tag="pgaptmp_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HGU4703118.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3531..4415)
                     /gene="nlpI"
                     /locus_tag="ACHKNP_RS04520"
                     /old_locus_tag="pgaptmp_000904"
     CDS             complement(3531..4415)
                     /gene="nlpI"
                     /locus_tag="ACHKNP_RS04520"
                     /old_locus_tag="pgaptmp_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HGU4703119.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4524..6659)
                     /gene="pnp"
                     /locus_tag="ACHKNP_RS04525"
                     /old_locus_tag="pgaptmp_000905"
     CDS             complement(4524..6659)
                     /gene="pnp"
                     /locus_tag="ACHKNP_RS04525"
                     /old_locus_tag="pgaptmp_000905"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGU4703120.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6906..7175)
                     /gene="rpsO"
                     /locus_tag="ACHKNP_RS04530"
                     /old_locus_tag="pgaptmp_000906"
     CDS             complement(6906..7175)
                     /gene="rpsO"
                     /locus_tag="ACHKNP_RS04530"
                     /old_locus_tag="pgaptmp_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGU4703121.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7324..8268)
                     /gene="truB"
                     /locus_tag="ACHKNP_RS04535"
                     /old_locus_tag="pgaptmp_000907"
     CDS             complement(7324..8268)
                     /gene="truB"
                     /locus_tag="ACHKNP_RS04535"
                     /old_locus_tag="pgaptmp_000907"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGU4703122.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8268..8669)
                     /gene="rbfA"
                     /locus_tag="ACHKNP_RS04540"
                     /old_locus_tag="pgaptmp_000908"
     CDS             complement(8268..8669)
                     /gene="rbfA"
                     /locus_tag="ACHKNP_RS04540"
                     /old_locus_tag="pgaptmp_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGU4703123.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8833..11505)
                     /gene="infB"
                     /locus_tag="ACHKNP_RS04545"
                     /old_locus_tag="pgaptmp_000909"
     CDS             complement(8833..11505)
                     /gene="infB"
                     /locus_tag="ACHKNP_RS04545"
                     /old_locus_tag="pgaptmp_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGU4703124.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11530..13017)
                     /gene="nusA"
                     /locus_tag="ACHKNP_RS04550"
                     /old_locus_tag="pgaptmp_000910"
     CDS             complement(11530..13017)
                     /gene="nusA"
                     /locus_tag="ACHKNP_RS04550"
                     /old_locus_tag="pgaptmp_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGU4703125.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13045..13497)
                     /gene="rimP"
                     /locus_tag="ACHKNP_RS04555"
                     /old_locus_tag="pgaptmp_000911"
     CDS             complement(13045..13497)
                     /gene="rimP"
                     /locus_tag="ACHKNP_RS04555"
                     /old_locus_tag="pgaptmp_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGU4703126.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13704..13780)
                     /locus_tag="ACHKNP_RS04560"
                     /old_locus_tag="pgaptmp_000912"
     tRNA            complement(13704..13780)
                     /locus_tag="ACHKNP_RS04560"
                     /old_locus_tag="pgaptmp_000912"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13744..13746),aa:Met,seq:cat)
     gene            14128..15471
                     /gene="argG"
                     /locus_tag="ACHKNP_RS04565"
                     /old_locus_tag="pgaptmp_000913"
     CDS             14128..15471
                     /gene="argG"
                     /locus_tag="ACHKNP_RS04565"
                     /old_locus_tag="pgaptmp_000913"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HGU4703127.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15479..17104)
                     /gene="yhbX"
                     /locus_tag="ACHKNP_RS04570"
                     /old_locus_tag="pgaptmp_000914"
     CDS             complement(15479..17104)
                     /gene="yhbX"
                     /locus_tag="ACHKNP_RS04570"
                     /old_locus_tag="pgaptmp_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4703128.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(17563..17649)
                     /locus_tag="ACHKNP_RS04575"
                     /old_locus_tag="pgaptmp_000915"
     tRNA            complement(17563..17649)
                     /locus_tag="ACHKNP_RS04575"
                     /old_locus_tag="pgaptmp_000915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17613..17615),aa:Leu,seq:gag)
     gene            complement(17664..17996)
                     /gene="secG"
                     /locus_tag="ACHKNP_RS04580"
                     /old_locus_tag="pgaptmp_000916"
     CDS             complement(17664..17996)
                     /gene="secG"
                     /locus_tag="ACHKNP_RS04580"
                     /old_locus_tag="pgaptmp_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGU4703129.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(18224..19561)
                     /gene="glmM"
                     /locus_tag="ACHKNP_RS04585"
                     /old_locus_tag="pgaptmp_000917"
     CDS             complement(18224..19561)
                     /gene="glmM"
                     /locus_tag="ACHKNP_RS04585"
                     /old_locus_tag="pgaptmp_000917"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGU4703130.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(19554..20402)
                     /gene="folP"
                     /locus_tag="ACHKNP_RS04590"
                     /old_locus_tag="pgaptmp_000918"
     CDS             complement(19554..20402)
                     /gene="folP"
                     /locus_tag="ACHKNP_RS04590"
                     /old_locus_tag="pgaptmp_000918"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGU4703131.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(20492..22426)
                     /gene="ftsH"
                     /locus_tag="ACHKNP_RS04595"
                     /old_locus_tag="pgaptmp_000919"
     CDS             complement(20492..22426)
                     /gene="ftsH"
                     /locus_tag="ACHKNP_RS04595"
                     /old_locus_tag="pgaptmp_000919"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGU4703132.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(22526..23155)
                     /gene="rlmE"
                     /locus_tag="ACHKNP_RS04600"
                     /old_locus_tag="pgaptmp_000920"
     CDS             complement(22526..23155)
                     /gene="rlmE"
                     /locus_tag="ACHKNP_RS04600"
                     /old_locus_tag="pgaptmp_000920"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HGU4703133.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            23281..23574
                     /gene="yhbY"
                     /locus_tag="ACHKNP_RS04605"
                     /old_locus_tag="pgaptmp_000921"
     CDS             23281..23574
                     /gene="yhbY"
                     /locus_tag="ACHKNP_RS04605"
                     /old_locus_tag="pgaptmp_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGU4703134.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(23730..24206)
                     /gene="greA"
                     /locus_tag="ACHKNP_RS04610"
                     /old_locus_tag="pgaptmp_000922"
     CDS             complement(23730..24206)
                     /gene="greA"
                     /locus_tag="ACHKNP_RS04610"
                     /old_locus_tag="pgaptmp_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGU4703135.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            24454..25887
                     /gene="dacB"
                     /locus_tag="ACHKNP_RS04615"
                     /old_locus_tag="pgaptmp_000923"
     CDS             24454..25887
                     /gene="dacB"
                     /locus_tag="ACHKNP_RS04615"
                     /old_locus_tag="pgaptmp_000923"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU4703136.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(25927..27099)
                     /gene="cgtA"
                     /locus_tag="ACHKNP_RS04620"
                     /old_locus_tag="pgaptmp_000924"
     CDS             complement(25927..27099)
                     /gene="cgtA"
                     /locus_tag="ACHKNP_RS04620"
                     /old_locus_tag="pgaptmp_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HGU4703137.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRIIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(27115..28080)
                     /gene="yhbE"
                     /locus_tag="ACHKNP_RS04625"
                     /old_locus_tag="pgaptmp_000925"
     CDS             complement(27115..28080)
                     /gene="yhbE"
                     /locus_tag="ACHKNP_RS04625"
                     /old_locus_tag="pgaptmp_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4703138.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYALCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(28207..28464)
                     /gene="rpmA"
                     /locus_tag="ACHKNP_RS04630"
                     /old_locus_tag="pgaptmp_000926"
     CDS             complement(28207..28464)
                     /gene="rpmA"
                     /locus_tag="ACHKNP_RS04630"
                     /old_locus_tag="pgaptmp_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGU4703139.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(28485..28796)
                     /gene="rplU"
                     /locus_tag="ACHKNP_RS04635"
                     /old_locus_tag="pgaptmp_000927"
     CDS             complement(28485..28796)
                     /gene="rplU"
                     /locus_tag="ACHKNP_RS04635"
                     /old_locus_tag="pgaptmp_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGU4703140.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            29055..30026
                     /gene="ispB"
                     /locus_tag="ACHKNP_RS04640"
                     /old_locus_tag="pgaptmp_000928"
     CDS             29055..30026
                     /gene="ispB"
                     /locus_tag="ACHKNP_RS04640"
                     /old_locus_tag="pgaptmp_000928"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HGU4703141.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            30253..30531
                     /gene="sfsB"
                     /locus_tag="ACHKNP_RS04645"
                     /old_locus_tag="pgaptmp_000929"
     CDS             30253..30531
                     /gene="sfsB"
                     /locus_tag="ACHKNP_RS04645"
                     /old_locus_tag="pgaptmp_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HGU4703142.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(30579..31838)
                     /gene="murA"
                     /locus_tag="ACHKNP_RS04650"
                     /old_locus_tag="pgaptmp_000930"
     CDS             complement(30579..31838)
                     /gene="murA"
                     /locus_tag="ACHKNP_RS04650"
                     /old_locus_tag="pgaptmp_000930"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGU4703143.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(31893..32147)
                     /gene="ibaG"
                     /locus_tag="ACHKNP_RS04655"
                     /old_locus_tag="pgaptmp_000931"
     CDS             complement(31893..32147)
                     /gene="ibaG"
                     /locus_tag="ACHKNP_RS04655"
                     /old_locus_tag="pgaptmp_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HGU4703144.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(32307..32600)
                     /gene="mlaB"
                     /locus_tag="ACHKNP_RS04660"
                     /old_locus_tag="pgaptmp_000932"
     CDS             complement(32307..32600)
                     /gene="mlaB"
                     /locus_tag="ACHKNP_RS04660"
                     /old_locus_tag="pgaptmp_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HGU4703145.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(32600..33235)
                     /gene="mlaC"
                     /locus_tag="ACHKNP_RS04665"
                     /old_locus_tag="pgaptmp_000933"
     CDS             complement(32600..33235)
                     /gene="mlaC"
                     /locus_tag="ACHKNP_RS04665"
                     /old_locus_tag="pgaptmp_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HGU4703146.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(33254..33805)
                     /gene="mlaD"
                     /locus_tag="ACHKNP_RS04670"
                     /old_locus_tag="pgaptmp_000934"
     CDS             complement(33254..33805)
                     /gene="mlaD"
                     /locus_tag="ACHKNP_RS04670"
                     /old_locus_tag="pgaptmp_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HGU4703147.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(33810..34592)
                     /gene="mlaE"
                     /locus_tag="ACHKNP_RS04675"
                     /old_locus_tag="pgaptmp_000935"
     CDS             complement(33810..34592)
                     /gene="mlaE"
                     /locus_tag="ACHKNP_RS04675"
                     /old_locus_tag="pgaptmp_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HGU4703148.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(34600..35409)
                     /gene="mlaF"
                     /locus_tag="ACHKNP_RS04680"
                     /old_locus_tag="pgaptmp_000936"
     CDS             complement(34600..35409)
                     /gene="mlaF"
                     /locus_tag="ACHKNP_RS04680"
                     /old_locus_tag="pgaptmp_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HGU4703149.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            35619..36596
                     /gene="yrbG"
                     /locus_tag="ACHKNP_RS04685"
                     /old_locus_tag="pgaptmp_000937"
     CDS             35619..36596
                     /gene="yrbG"
                     /locus_tag="ACHKNP_RS04685"
                     /old_locus_tag="pgaptmp_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HGU4703150.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            36610..37596
                     /gene="kdsD"
                     /locus_tag="ACHKNP_RS04690"
                     /old_locus_tag="pgaptmp_000938"
     CDS             36610..37596
                     /gene="kdsD"
                     /locus_tag="ACHKNP_RS04690"
                     /old_locus_tag="pgaptmp_000938"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HGU4703151.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            37617..38183
                     /gene="kdsC"
                     /locus_tag="ACHKNP_RS04695"
                     /old_locus_tag="pgaptmp_000939"
     CDS             37617..38183
                     /gene="kdsC"
                     /locus_tag="ACHKNP_RS04695"
                     /old_locus_tag="pgaptmp_000939"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HGU4703152.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCVLTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            38180..38755
                     /gene="lptC"
                     /locus_tag="ACHKNP_RS04700"
                     /old_locus_tag="pgaptmp_000940"
     CDS             38180..38755
                     /gene="lptC"
                     /locus_tag="ACHKNP_RS04700"
                     /old_locus_tag="pgaptmp_000940"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HGU4703153.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            38724..39281
                     /gene="lptA"
                     /locus_tag="ACHKNP_RS04705"
                     /old_locus_tag="pgaptmp_000941"
     CDS             38724..39281
                     /gene="lptA"
                     /locus_tag="ACHKNP_RS04705"
                     /old_locus_tag="pgaptmp_000941"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HGU4703154.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            39288..40013
                     /gene="lptB"
                     /locus_tag="ACHKNP_RS04710"
                     /old_locus_tag="pgaptmp_000942"
     CDS             39288..40013
                     /gene="lptB"
                     /locus_tag="ACHKNP_RS04710"
                     /old_locus_tag="pgaptmp_000942"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HGU4703155.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            40061..41494
                     /gene="rpoN"
                     /locus_tag="ACHKNP_RS04715"
                     /old_locus_tag="pgaptmp_000943"
     CDS             40061..41494
                     /gene="rpoN"
                     /locus_tag="ACHKNP_RS04715"
                     /old_locus_tag="pgaptmp_000943"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HGU4703156.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            41517..41804
                     /gene="hpf"
                     /locus_tag="ACHKNP_RS04720"
                     /old_locus_tag="pgaptmp_000944"
     CDS             41517..41804
                     /gene="hpf"
                     /locus_tag="ACHKNP_RS04720"
                     /old_locus_tag="pgaptmp_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HGU4703157.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            41922..42413
                     /gene="ptsN"
                     /locus_tag="ACHKNP_RS04725"
                     /old_locus_tag="pgaptmp_000945"
     CDS             41922..42413
                     /gene="ptsN"
                     /locus_tag="ACHKNP_RS04725"
                     /old_locus_tag="pgaptmp_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HGU4703158.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            42459..43313
                     /gene="rapZ"
                     /locus_tag="ACHKNP_RS04730"
                     /old_locus_tag="pgaptmp_000946"
     CDS             42459..43313
                     /gene="rapZ"
                     /locus_tag="ACHKNP_RS04730"
                     /old_locus_tag="pgaptmp_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGU4703159.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            43310..43582
                     /gene="npr"
                     /locus_tag="ACHKNP_RS04735"
                     /old_locus_tag="pgaptmp_000947"
     CDS             43310..43582
                     /gene="npr"
                     /locus_tag="ACHKNP_RS04735"
                     /old_locus_tag="pgaptmp_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HGU4703160.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            43796..44428
                     /gene="yrbL"
                     /locus_tag="ACHKNP_RS04740"
                     /old_locus_tag="pgaptmp_000948"
     CDS             43796..44428
                     /gene="yrbL"
                     /locus_tag="ACHKNP_RS04740"
                     /old_locus_tag="pgaptmp_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HGU4703161.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(44425..45153)
                     /gene="mtgA"
                     /locus_tag="ACHKNP_RS04745"
                     /old_locus_tag="pgaptmp_000949"
     CDS             complement(44425..45153)
                     /gene="mtgA"
                     /locus_tag="ACHKNP_RS04745"
                     /old_locus_tag="pgaptmp_000949"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HGU4703162.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(45150..45803)
                     /gene="elbB"
                     /locus_tag="ACHKNP_RS04750"
                     /old_locus_tag="pgaptmp_000950"
     CDS             complement(45150..45803)
                     /gene="elbB"
                     /locus_tag="ACHKNP_RS04750"
                     /old_locus_tag="pgaptmp_000950"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HGU4703163.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(46033..48369)
                     /gene="arcB"
                     /locus_tag="ACHKNP_RS04755"
                     /old_locus_tag="pgaptmp_000951"
     CDS             complement(46033..48369)
                     /gene="arcB"
                     /locus_tag="ACHKNP_RS04755"
                     /old_locus_tag="pgaptmp_000951"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HGU4703164.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(48465..49394)
                     /gene="yhcC"
                     /locus_tag="ACHKNP_RS04760"
                     /old_locus_tag="pgaptmp_000952"
     CDS             complement(48465..49394)
                     /gene="yhcC"
                     /locus_tag="ACHKNP_RS04760"
                     /old_locus_tag="pgaptmp_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HGU4703165.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            50069..54529
                     /gene="gltB"
                     /locus_tag="ACHKNP_RS04765"
                     /old_locus_tag="pgaptmp_000953"
     CDS             50069..54529
                     /gene="gltB"
                     /locus_tag="ACHKNP_RS04765"
                     /old_locus_tag="pgaptmp_000953"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HGU4703166.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            54542..55960
                     /gene="gltD"
                     /locus_tag="ACHKNP_RS04770"
                     /old_locus_tag="pgaptmp_000954"
     CDS             54542..55960
                     /gene="gltD"
                     /locus_tag="ACHKNP_RS04770"
                     /old_locus_tag="pgaptmp_000954"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HGU4703167.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNPLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            56144..57191
                     /gene="yhcG"
                     /locus_tag="ACHKNP_RS04775"
                     /old_locus_tag="pgaptmp_000955"
                     /pseudo
     CDS             56144..57191
                     /gene="yhcG"
                     /locus_tag="ACHKNP_RS04775"
                     /old_locus_tag="pgaptmp_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
     gene            complement(57251..57715)
                     /gene="nanQ"
                     /locus_tag="ACHKNP_RS04780"
                     /old_locus_tag="pgaptmp_000956"
     CDS             complement(57251..57715)
                     /gene="nanQ"
                     /locus_tag="ACHKNP_RS04780"
                     /old_locus_tag="pgaptmp_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HGU4703168.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(57712..58587)
                     /gene="nanK"
                     /locus_tag="ACHKNP_RS04785"
                     /old_locus_tag="pgaptmp_000957"
     CDS             complement(57712..58587)
                     /gene="nanK"
                     /locus_tag="ACHKNP_RS04785"
                     /old_locus_tag="pgaptmp_000957"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HGU4703169.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(58584..59273)
                     /gene="nanE"
                     /locus_tag="ACHKNP_RS04790"
                     /old_locus_tag="pgaptmp_000958"
     CDS             complement(58584..59273)
                     /gene="nanE"
                     /locus_tag="ACHKNP_RS04790"
                     /old_locus_tag="pgaptmp_000958"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGU4703170.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(59321..60811)
                     /gene="nanT"
                     /locus_tag="ACHKNP_RS04795"
                     /old_locus_tag="pgaptmp_000959"
     CDS             complement(59321..60811)
                     /gene="nanT"
                     /locus_tag="ACHKNP_RS04795"
                     /old_locus_tag="pgaptmp_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HGU4703171.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(60921..61814)
                     /gene="nanA"
                     /locus_tag="ACHKNP_RS04800"
                     /old_locus_tag="pgaptmp_000960"
     CDS             complement(60921..61814)
                     /gene="nanA"
                     /locus_tag="ACHKNP_RS04800"
                     /old_locus_tag="pgaptmp_000960"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGU4703172.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(61936..62727)
                     /gene="nanR"
                     /locus_tag="ACHKNP_RS04805"
                     /old_locus_tag="pgaptmp_000961"
     CDS             complement(61936..62727)
                     /gene="nanR"
                     /locus_tag="ACHKNP_RS04805"
                     /old_locus_tag="pgaptmp_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HGU4703173.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        15390 a        17088 c        15524 g        14877 t
ORIGIN      
        1 ctgataagcg aagcgcatca ggcattgtgt aggcagcaga aatgtcggat aaggcaccgc
       61 tgattactga tacaccggca gtaaattaaa gctcgataaa atatggacca gtgcgttgcc
      121 aacgccaaac accagaatca gcgtaatcat cggcttgccg ccccagacgc ggaatttcgg
      181 gctgccaaag cgtttacgcg atgcgcgggc taacagcgcc ggaacaattg ccgcccagat
      241 ggtagctgct aaaccagcat aaccaatggc gtacaggaat ccgttcggga acagtagacc
      301 acccacaaca ggcggggcaa aggtcagcaa tgccgttttc aagcgcccca gggctgagtc
      361 atcgaaacca aacagatctg ccagatagtc aaacaaaccc agcgttacgc caaggaacga
      421 actcgctacc gcaaagtttg agaacacgac cagcagaagg tccaggctac ggctgttcag
      481 cacgccgctt aatgcctgta ccagcacatc aatattaccg cccttctctg caataccgat
      541 aaactccgga cgcgggatgt tacccatcgt cgccagcaac cagatggtat acagcgccag
      601 tgccatcagc gtaccgtaca ccaggcattt cacgatggtt ttcggatctt tgccgtaata
      661 cttcatcagg cttggcacgt taccgtgata accaaacgac gccaggcaga acggcaatgt
      721 catcagcaga tacggcgcat aagatgcatt gctttctgca acgttgaaca atgtcgcagg
      781 ctgcacatgc cccaacaggc tgccaaaggt gaggaagaag gtaatgactt tcgcccccag
      841 cacaatcgcc gtcatgcggc tgacggcctt agtactcaac cacaccacaa acgctaccag
      901 cagtgcaaaa ccaaaacccg ccgcccgtgc cgggacgttt agcgacatct ctgcgaaggt
      961 gtgatgcaga attgaaccac tggcagaaat ataggcatag gtcaggatat agagcacaaa
     1021 ggcaatggaa ataccgttga ccacgttcca gcctttgccc agcaaatctt tggtgatggt
     1081 gtcaaaactc gaaccgattc ggtaattcag gttagcttcc agaatcatca agccggaatg
     1141 cagcatacag aaccaggtaa agatcagcgc cgccattgac cagaaaaacc acgccccgga
     1201 catgaccact ggcagagaaa acatccctgc gccaataatg gtgccgccga taatcaccac
     1261 gccgccaagc agcgacggtg acgtttgggt ggtggttagt gttgccatga gggcttctct
     1321 ccagtgaaaa atagtgcgac tgcgttgtta tgcattgcac tgtaccagta cacgagtaca
     1381 aaagacagaa aaaaagcccc gatggtaaaa atcggggctg tatatattat tttacagatt
     1441 gtgttcgctg ttcagcgatg attacgcatc accaccgaaa cgacgacgac cggtagagtc
     1501 atcacgacgc ggagcgcggc cttcacgacg ttcgccgcta aaacgacgac catcaccacg
     1561 gccaccttca cggcgttcac cgctgaagtt acgaccgcct tcacgacgtt cgccaccgaa
     1621 accacgacca ccgccacgac gctcaccgcc agtatgcggc tgcgcatcgc ccagcaactg
     1681 catattcatc ggcttgttca gaatgcgagt acgcgtaaag tgttgcagta cttcacccgg
     1741 catacctttc ggcagttcga tagtggagtg agaagcaaac agcttaatgt taccgatgta
     1801 acggctgctg atgtcgcctt cgttagcaat cgcgccaacg atatgacgaa cttcaacacc
     1861 atcatcgcgg cccacttcaa tgcggtaaag ctgcatgtca ccaacatcac gacgttcacg
     1921 acgcggacga tcttcacggt cgccacgtgg gccacggtcg ttacgatcgc gcggaccacg
     1981 gtcatcacgg tcacggaatt cacgtttcgg acgcatcggc gcatctggcg gaacaatcag
     2041 agtacgttca ccctgtgcca ttttcagcag tgccgcagcc agagtttcga gatcctgctc
     2101 ttcaccttct gcagtcggct gaattttgct cagcagtgcg cggtattgat ccagatcgct
     2161 gctttccagc tgctgctgta ctttagcggc gaatttttcc agacggcgtt tgcccagcag
     2221 ttctgcgttc ggcagttcta cttccggaat agtcagcttc atagtacgtt caatgttgcg
     2281 cagcagacga cgctcgcggt tctcaacgaa cagcagcgcg cggccagcac gacccgcacg
     2341 accggtacga ccgatacggt gaacgtaaga ctcagaatcc atcgggatat cgtagttaac
     2401 taccaggctg atacgctcaa cgtccaggcc acgggctgca acgtcggtcg caatcaggat
     2461 gtccagacga ccatctttca ggcgttccag tgtctgttca cgcagcgcct ggttcatgtc
     2521 accgttcagc gcggcgctgt tgtagccgtt acgctcaaga gcttcagcca cttccagagt
     2581 cgcgtttttg gtacgaacga agataatcgc cgcatcaaaa tcttccgctt ccaggaaacg
     2641 taccagtgct tcgtttttgc gcatacccca gacagtccag tagctctggc tgatgtcagg
     2701 acgggtagtc acgctggact gaatgcgcac ttcctgcggc tctttcataa agcggcgggt
     2761 aatgcgacga atcgcttccg gcatggttgc agagaacaga gcggtctgat gaccttccgg
     2821 gatctgcgcc ataatggttt caacgtcttc gatgaagccc atgcgcagca tttcgtcagc
     2881 ttcatccaga accagaccgc tcagtttaga gaggtccaga gtgccacgtt tcaggtggtc
     2941 cagcagacgg cccggagtac cgacaacgat ctgcggcccc tgacgcaggg cgcgtaattg
     3001 cacgtcataa cgctggccgc cgtatagagc aaccacattt acgccgcgca tgtgtttaga
     3061 gaaatccgtc attgcttcag caacctgtac cgccagttcg cgggtcggtg ccagcaccag
     3121 aatctgtggt gctttcagct caggatcaag attctgcaac agaggtaaag agaatgctgc
     3181 agtttttccg ctccccgtct gggccatacc cagaacgtcg cggccattca gcagatgtgg
     3241 aatacactct gcctgaattg gagatggttt ttcgtaaccc agatcgttaa gggcttcaag
     3301 gataggagcc ttcaggccca gatctgcaaa agtggtttcg aattcagcca tgtagtacgt
     3361 gtgcctcaaa attaatggcg gccagtctac ataactcatc atgaaattga tcagcaattt
     3421 tcattgaaaa gtgtgaaccg gctcaaagta ggtgtattaa cgaacaacaa cgccctcacc
     3481 cgttaaggtg atggcaatca aaaaagatta cgggctgatg tgtacgtcag ctattgctgg
     3541 tccgattctg ccaggtcatc ttggtcctgg cccaggagcg ataattccaa caatgcgtat
     3601 cggtgctcaa caaagttatg aacgttgttg gcaaccgcca gtttgaacag tgccgtggcg
     3661 ctgtccaaat cccccagact taggtagtac ttacctaaat agaagttggt ttcactgaga
     3721 tgctcagcga gcgaggtgtt atccgttgcg tccgccttga gcctttccat taacgtttgt
     3781 tcgctaatgt tgcccaggta gaactcgaca atgttccatc cccactgttc cttatccgat
     3841 ttttcgaagt gctgtttcaa cacttcttta gcctgcttct catcgagctt ctgctcggcg
     3901 agataaagcc acagactacg gaaaggatca ttgggatcgt cttgataaaa cgccagcaga
     3961 tcatcttgcg ctaacttgtc acgaccgccg taatataatg cgatcccgcg attcaagtgc
     4021 gcgtagttgt aagttggatc aagctcaagt acagaatcaa acgcttcata ggcagcatca
     4081 aaattgcctg cctgcgttaa atatatgcct aagtaattga atacttcagg catatccggt
     4141 cggattgcca gcgcttgcga aaaatcgtta cgcgctaatg ccctcagacc gagactatca
     4201 tacaacactc cgcgctcata taaaagctgt gcgcgttcgt catcggttaa agcccgactg
     4261 gcaaggattt gttccatacg tgccagaatc acttcctgct gtaaagtcgg ttgcaatggt
     4321 accgcgagga cttcactttt acgccaggaa gtattactgc atcctgcaag cgtaagtgct
     4381 gtcgcaacga aacaccagcg caaaaaaggc ttcatttccc actcccgaag accacggttg
     4441 aatgaacgtc ctgttcccgg ttgctaacaa ggcgtcctgc ccggttaaaa gccccccgcc
     4501 gcagcggagg gcaaatggca accttactcg ccctgttcag cagccggagc ttccggtgct
     4561 gcagcaggtt gagactgctc agtcgcttct ttaatgctca gacggatacg gccctggcga
     4621 tcaacttcca gaactttcac cggtacttcc tgacccatct gcaggtaatc ggtcactttc
     4681 tcaacgcgtt tgtcagcgat ttgagagatg tggaccagac cttctttacc gccgccgatg
     4741 gcaacaaatg cgccaaagtc aacgatacgg gtcactttac cagtgtagac gcggcccact
     4801 tcgatttctg cagtgatctc ttcgatacga cgaatagcat gtttcgcttt ctcgccgtcg
     4861 gtcgctgcga tcttcacagt accgtcatct tcgatttcga tggtagtgcc agtttcttcg
     4921 gtcagggcac ggataacaga gccgccttta ccgataacgt ctttgatctt gtccgggttg
     4981 atcttgatgg tatggatacg cggtgcgaac tcagagatat cgccacgcgg cgcgttgatc
     5041 gcctgttcca ttacgcccag gatatgcaga cgcgcacctt tagcctggtt cagcgcaacc
     5101 tgcatgatct ctttggtgat accttcaatt ttgatatcca tctgcaatgc agagataccc
     5161 tcgcgggaac ccgcaacttt gaagtccata tcgcccaggt gatcttcgtc gcccaaaatg
     5221 tcagacagta caacgtagtt gtcgccttct ttcaccagac ccattgcgat acccgcaacg
     5281 gcagctttga tcggcacacc tgcgtccatc agcgccagag acgcgccgca cacggaagcc
     5341 atagaagagg aaccgttgga ttcagtgatt tcagacacaa cacgtacggt gtacgggaat
     5401 ttgtccatat ccggcatgac tgccagcacg ccgcgcttcg ccagacgacc gtgaccaatt
     5461 tcacgacgct tcggagaacc gaccatgccg gtttcgccta cggagtacgg agggaagttg
     5521 tagtggaaca ggaaggtatc ggtacgttcg cccatcagtt catcaagaac ctgcgcgtca
     5581 cgagcagtac ccagcgttgc ggtaaccagc gcctgcgttt caccacgggt gaacagcgca
     5641 gaaccgtgag tacgcggcag cacgccagta cgcacatcca gaccacggat catatctttt
     5701 tcacgaccgt cgatacgcgg ttcgcctgcc agtacgcggc tacgaacaac gtttttctcg
     5761 atagcgtgca gaatttcacc cagttcgttt tcgtccaggg tttcgtcttc agcaagcagc
     5821 gtcgcgatgg tttcagattt gatgacatca acctgcgcat aacgctcttg tttgtcggtg
     5881 atgcggtaag catcgctcag gcgagcttca gccagtgcag caacgcgcgc gttcagcgct
     5941 tcgtttaccg gctccggctg ccagtcccaa cgtggtttac cggcttcttt caccagttca
     6001 ttgatgttct gaataacaac ctgctgttgt tcatgaccga acactactgc gcccagcatc
     6061 tggtcttcgc tcagcagttc agcttcagat tcaaccatca gaacagcggc ttcagtaccg
     6121 gcaacaacca gatccagttt gctctctttc agctcgtcct gagtcgggtt cagtacgtac
     6181 tggtcattga tgtaacctac gcgagcagca ccaatcggac cattgaacgg aataccagac
     6241 agagacagtg ctgcggaagc accaatcatc gcgacgatat ccgggttaac ttgcgggtta
     6301 acagaaacca cggtggcgat aacctgaact tcgttgacga agccttccgg gaacagcggg
     6361 cgaatcgggc ggtcaatcag acgcgcgatc agggtttcgc cttcgcttgg gcggccttca
     6421 cgacggaaga agctacccgg gatacgacca gcagcgtagg tacgctcctg atagttaacg
     6481 gtcagtggga agaagtcctg acctggtttg gcttttttct ggccaacaac ggtaacaaat
     6541 accgcggtgt catccatgct aaccataaca gcggcagtag cctgacgagc catcatgccg
     6601 gtttccagag tcacggtgtg ttggccgtac tggaatttac gaacgatcgg attaagcaat
     6661 gtaatatcct ttctctttct tagacagtac cttacggcac tggtgttaat acccgatctt
     6721 ctgcgcatcc tcgcgactaa tgacaaccct aacccagctc tatgtgggta aagcctctca
     6781 ttagccgcgc gaacctctgc aacggaagat cattcatagc aacaatacat tagtttccag
     6841 tgaattgctg ccgtcagctt gaaaaaaggg gccactcagg cccccttttc tgaaactcgc
     6901 aagaattagc gacgcagacc caggcgctcg atgagctggg tgtaacgtgc tacgtcttta
     6961 cgtttcaggt agtcgagcag tttacgacgc tgagaaacca tgcgcagcag accacgacgg
     7021 ctgtggtgat cttttttgtg ctctgcaaag tggccctgca ggtggttgat ctgtgcagtc
     7081 agcagtgcta cctgaacttc ggtagaaccg gtgtcgtttg cgtcacgacc aaactcagaa
     7141 acgattttag ctgttgcttc agtacttaga gacattttaa aactccaaag tatatagaat
     7201 gaaaggacgc cgatctctaa ttcagcgatc ccagtgtacg ttacgcaaag tgttaaacaa
     7261 tttacgcgac gttaagcggc agtattctac tcgtagcgac ctgttatcgc aagacggtta
     7321 acattacgcc gggtattcaa ccaccaggcg acgaggcgca acgcggcctt catcgtcaat
     7381 ttcgcccata ccgataaatt tgccgttctc accttctgta acgcgaacca gtccttccag
     7441 cggcgcaccg gatgtacgaa ctgggttacc atttttgaag taaacagaag acgttaacgg
     7501 aagattcacc accgggtagt ccgaagctgg actgtccatt ggcatcagta atggatcaag
     7561 taactccgca gccggaatat cctgctgttc ggcttgttcg accagttcac gcagatgttc
     7621 cagggtcacc atccgttcaa ccggatattt acttaccgcc agacgacgca ggtaaataac
     7681 atgcgcgcca caaccgagtt tttcacccag gtcatcaatg atggtgcgga tataagtgcc
     7741 ttttgagcag tgaatttcca gctccagctc attgccttcg tggcgaataa acagcaattc
     7801 ataaacggta atcgggcgcg cttcacgcgg tacttcaatg ccctgacgcg catattcgta
     7861 cagtttttta ccctgatatt tcagtgctga atacatcgaa gggatctgtt cgatatcgcc
     7921 acggaaagta tccagcgccg ccgccagttg ctctgcgcta aaggttaccg gacgttcttc
     7981 aacgatttgt ccgtcggcat cagaagtatc ggtacgctgt ccaagacgcg caatgacccg
     8041 atagcgtttg tcggagtcca gcagatactg ggaaaacttc gtcgcttccc cgaggcaaat
     8101 tggcaacatg ccagttgcca gcgggtccag cgcaccggta tgcccggcac ggttggcgtt
     8161 ataaatccgt ttcacttttt gcagcgcatc attgctggac ataccctgcg atttatccag
     8221 caacaaaacg ccgttaatgt cgcgaccgcg acgacgagga cgactcatta gtcctccttg
     8281 ctgtcgtccg ggttaacacg acgttcttcg tcatgtttga ccacgctggt caccaggttt
     8341 gacatgcgca tcccttcaac cagagagttg tcgtagaaga aggtcagttc cggcacgata
     8401 cgcaggcgca tcgctttccc cagcaggctg cggatgaaac cagaagcttc ttgcaacgct
     8461 ttgatgcccg ctttaaccgc gtcttcatct ttgtcgttga ggaacgtcac atatactttg
     8521 gcatacgcca ggtcgcgaga catttcgaca ccggaaacgg tggtcatcat gcccaggcga
     8581 ggatctttaa tttcacgctg caggatgaga gcgatctctt tttgcatttc ctgcgctacg
     8641 cgctgcgggc gaccaaattc tttcgccata ataaattctc ctgacaaaaa aggggctgtt
     8701 agcccctttt taaaattaat ttcaggtgga agggctgttc acgttggcct gataagacgc
     8761 gccagcgtca catcaggcaa tccatgccgg atgcggcata aacgccttat cccgcatgga
     8821 accctaaaaa ccttaagcga tggtacgttg gatctcgatg atttcgaata cttcgatcac
     8881 atcgccagtg cggacgtcgt tgtagttctt aacgccgata ccacattcca taccgttacg
     8941 gacttcgtta acgtcatctt tgaagcggcg cagggactcc agctcgcctt cgtagataac
     9001 cacgttgtca cgcagaacgc ggatcgggtt gtgacgttta accacacctt cggtaaccat
     9061 acagcctgcg atggcaccaa atttcggcga tttgaacacg tcacgaactt ccgccagacc
     9121 gataatctgc tgtttcagtt ccggagacag cataccgctc atcgccgctt tcacttcgtc
     9181 aatcaggtta tagatgacgg agtagtaacg cagatccagg ctttccgctt caatcacttt
     9241 acgtgcagag gcatcagcac gtacgttgaa gccaaccagg atggcgttgg acgccgcagc
     9301 cagggtggcg tcggtttcgg tgataccacc tacgccagaa ccgatgatct tcactttaac
     9361 ttcgtcagta gacagtttca gcaaggagtc ggagatcgct tccacagaac cctgtacgtc
     9421 tgccttcagg acgatgttca cttcgtgaac ttcgccttcg gtcatgttgg cgaacatgtt
     9481 ctcgagttta gatttctgct gacgcgccag tttaacttcg cggaatttac cctgacgata
     9541 cagtgcaact tcacgcgctt tcttctcgtc acgtacaacg gtaacttcat cacccgcagc
     9601 cggtacgccg gacaggccga ggatttccac cggaatggac ggacccgctt ccagcacttc
     9661 ctgacccagt tcgttacgca tcgcacgaac acgaccgtat tcgaagccac acagaacgat
     9721 atcgcccttg tgcagagtac cttcacgtac cagaacggta gcaaccggac cacgaccttt
     9781 atcgaggaag gattcgataa ccgcaccgct cgccatacct ttacgtaccg ctttcagctc
     9841 cagaacttcc gcctgcagca ggatagcgtc cagcagttca tcgatgccgg tacccgcttt
     9901 cgcagatacg tgtacgaact ggctttcacc gccccactct tccggcagga tgccatactg
     9961 ggagagttcg ttcttaacac gatccggatc ggcttctggt ttatcaatct tgttcactgc
    10021 aaccaccacc ggtacctgcg ccgctttcgc gtgctggatt gcttcgatgg tctgcggcat
    10081 cacgccgtcg tcggcagcaa caaccaggac tacgatgtcc gttgcctgcg caccacgtgc
    10141 acgcattgaa gtaaacgcgg cgtgccccgg ggtgtccagg aaggtgatca tgccgttttc
    10201 agtttcaacg tggtatgcac caatgtgctg ggtaatgccg cccgcttcgc cagaggcaac
    10261 tttcgttgaa cgaatgtagt ccagcagaga ggttttaccg tggtcaacgt gacccatgat
    10321 ggtcacaacc ggcgcgcgcg gttcagccgc agcacccgtg tcacggtcgc tcattaccgc
    10381 ctcttccagc tcgttttcac gacgcaggat aactttgtgg cccatctctt cagcaaccag
    10441 ctgtgcggtt tcctgatcga taacctggtt gatggttgcc attgcgccca gtttcatcat
    10501 cgctttgatg acctgagagc ctttaaccgc catcttgttc gccagttcgc caacggtgat
    10561 ggtttcgccg atcacaacgt cacggttaac ggcctgagct ggcttctgga agccttgctg
    10621 cagggaagaa cctttacgtt ttccgccttt accgccacgt actgctgcgc gtgcttcttc
    10681 acgatcagct tttgattcag cgtgtttgtt gcctttcttc ggacgcgctg ctttcgcgtt
    10741 acgaccacgg ccacgaccgc cttcgacttc acgatcgctt tcgtcttctg cctggcgagc
    10801 atgttgagaa gtagtgacgt gataatcgct ggaatcttca gtcggttccg cgttatcagt
    10861 ccatttgttt tcttccgcca tacgacgtgc ttcttcagca acgcgacgtg cttcttcttc
    10921 gagtttacga cgcgcttctt cttcagcttt acgcttgagc tctgcagctt cctgctcacg
    10981 gcgggctttt tcagcctggg cgtttttagt catatcgtct tgttgattgc tcactttgtc
    11041 tttttccgca gcttcacgtt tcgcttgttc agcagcttca cgcttagctt gttctgcggc
    11101 ctcacgttca gctttttgtt gcgcctcgcg tttagccgat tcttctgcct cacgacgggc
    11161 ttgctcttcc gcttcacgct gcgcttgctc ttccgctgca aggcgttcag cctcttgcgg
    11221 atcgcgtttc acaaaggtgc gtttcttgcg gacttcgatt tgtaccgatt tgctttttcc
    11281 accggtacca ggaatgttaa gggtgctgcg tgttttacgt tgcagcgtca atttgtccgg
    11341 gcctgaattt ttctgattca ggtggtcaat caaagtctgt ttctcttgtg cagacacaga
    11401 gtcgtcagca gacttccgga tacctgcatc agcaaattgc tgtaccaggc gttccacgga
    11461 ggtctgtcgc tctgcggcca gcgttttaat cgttacatct gtcatgctgt tccttcctgc
    11521 tacagtttat tacgcttcgt caccgaacca gcaaatatta cgggcagcca taatcagtgc
    11581 tccggctttt tcgtcggtca acccttcgat atcagccaga tcatcaatgc cctgttcggc
    11641 gagatcttcc agcgtacaaa cgccacgggc ggccagtttg aatgccaaat cacgatctat
    11701 cccttcaagg ttcagcagat cgtcagccgg tttgttatca ccgaggcttt cttcctgggc
    11761 ctgtgcaatg gtggccagtg catttttagc acgctcgcgc agtgcttcaa cggtcggctc
    11821 atcaaggcct tcgatttcca acagctcttt catcggcaca taggccagtt cttccagcgt
    11881 cgagaagcct tcttctacca gaacagtcgc gaagtcttcg tcgatgtcga gatatttggt
    11941 gaaggtgtcg atcgctgcgt gcgcttccgc ctgatgctta gcctgcaggt cgtcaacggt
    12001 catcacgttg agttcccaac cgctcagctg cgaagccaga cgcacgttct gaccgttacg
    12061 gccaatcgcc tgcgccaggt taccggcttc aacggcgata tccatggtgt gtttatcttc
    12121 atccaccacg atagaagcaa cgtctgccgg tgccattgcg ttaatcacaa actgcgccgg
    12181 gttatcatcc cacaggacga tatcgatacg ctcgccgccc agttcagtag aaaccgcctg
    12241 aacacgcgcg ccacgcatac ctacacaggc acctaccgga tcgatacgtt tatcgttggt
    12301 tttcaccgcg attttcgcac gagaacccgg atcgcgagcc gctgctttaa tttcaatcac
    12361 ttcttcgccg atttctggca cttcaatacg gaacagttcg atcagcattt ccggcttgga
    12421 acgagtgacg aacagttgcg cgccacgcgc ttccgggcga acggaataga gcacgccacg
    12481 aacgcggtcg ccagggcgga agttttcacg cggcagcata tcttcgcgca ggatcacggc
    12541 ttcagcattg ttgcccagat ccagagagat gttgtcgcgg tttacttttt tcaccacgcc
    12601 ggtgatgatt tcaccttcgt gttcacggaa ctgatcaacc accatcgcac gttcggcttc
    12661 acgcactttc tgcacgataa cctgttttgc cgtctgggta gtgatacggt caaaggtaac
    12721 agactcaatc tgatcttcaa cgtaatcgcc caggttcagg ctttcatctt cataacgtgc
    12781 ggcttcaagg gtgatttcct tggtcggctg ggtgacttca tcaacaacta accagcgacg
    12841 gaaggtgtca aaatcaccgc ttttgcgatc gatctgtacg cggacgtcga tctcttgttc
    12901 atattttttc tttgttgctg tcgccagcgc gctttccaat gcttcgaaaa tcttctcgcg
    12961 aggtagcgcc ttttcattgg atacggcttc aactacagcc aaaatttctt tgttcatcgc
    13021 gggcttttca cctcatccag actattaaaa gtggggaacc aggttcgcct tctggatatt
    13081 actcagcgcg aacacttcat ctttaccttc gacggtaact gtgatcattt caccgtctac
    13141 cgctttgata acgccctgcc atttacgacg gttttgtacc gccatacgga gaaccagagt
    13201 cacctcttct ccgacaaaac gggcgtagtg ttcagccgtg aacagtgggc gatcgagacc
    13261 cggtgaggag acttccaggt tataagcaac ggtgatggga tcttcaacat ccagcacagc
    13321 acttacctgg tggctcacat cagcacaatc atcaacattg atgccatctt cactatcaat
    13381 atagatgcgc agtgtggatg tgcgaccgcg aataaattcg atgccaacca gttcaaaacc
    13441 cagggcctca actggcgcag taatcatctc tgttaatttt tgctctaatg tggacaagcc
    13501 cacccccaag acataaaaaa agggcctaaa gcccagttat tctgtagtca gataacaaaa
    13561 aaccccgata aatcggggct ttatataact gaaccctata accgcaactg cggtctggag
    13621 cactttccag aaggattttt tcaaatccca ctacgaaggc cgaagtcttc acagtatatt
    13681 tgaaaaagga ctctaaggga aagtggttgc gggggccgga tttgaaccga cgaccttcgg
    13741 gttatgagcc cgacgagcta ccaggctgct ccaccccgcg cctgaaacgt ggcaaattct
    13801 actcgttttg ggtaaaagat gcaaatactg ctgggatttg gtgtaccgag acgggacgta
    13861 aaatctgcag gcattatagt gatccatgcc acattttgtc aacgtttatt gctaataacg
    13921 tgaatgaata tccagttcac tttcatttgt tgaatacttt tgccttctcc tgctctccct
    13981 taagcgcatt attttacaaa aaacacacta aactcttcct gtctccgata aaagatgatt
    14041 aaatgaaaac tcatttattt tgcataaaaa ttcagtgaga gcggaaatcc aggctcatca
    14101 tcagttaatt aagcagggtg ttattttatg acgacgattc tcaagcatct cccggtaggt
    14161 caacgtattg gtatcgcttt ttccggcggt ctggacacca gtgccgcact gctgtggatg
    14221 cgacaaaagg gagcggttcc ttatgcatat actgcaaacc tgggccagcc agacgaagag
    14281 gattatgatg cgatccctcg tcgtgccatg gaatacggcg cggagaacgc acgtctgatc
    14341 gactgccgca aacaactggt ggccgaaggt attgccgcta ttcagtgtgg cgcatttcat
    14401 aacaccaccg gcggcctgac ctatttcaac acgacgccgc tgggccgcgc cgtgactggt
    14461 accatgctgg ttgctgcgat gaaagaagat ggcgtgaata tctggggtga cggtagcacc
    14521 tataaaggaa acgatatcga acgtttctat cgttatggtc tgctgaccaa tgctgaactg
    14581 cagatttaca aaccgtggct tgatactgac tttattgatg aactgggcgg ccgtcatgag
    14641 atgtctgaat ttatgattgc ctgcggtttc gactacaaaa tgtctgtcga aaaagcctac
    14701 tccacagact ccaacatgct tggtgcaacg catgaagcga aggatctgga atacctcaac
    14761 tccagcgtca aaatcgtcaa cccgattatg ggcgtgaaat tctgggatga gagcgtgaag
    14821 atcccggcag aagaagtcac agtacgcttt gagcaaggtc atccggtggc gctgaacggt
    14881 aaaaccttta gcgacgacgt agaaatgatg ctggaagcta accgcatcgg cggtcgtcac
    14941 ggcctgggca tgagcgacca gattgagaac cgtatcatcg aagcgaaaag ccgtggtatt
    15001 tacgaagctc cggggatggc actgctgcac attgcgtatg aacgtctgtt gaccggtatt
    15061 cacaacgaag acaccattga gcagtatcac gcgcatggtc gtcagttggg ccgtctgctg
    15121 taccaggggc gttggtttga ctcccaggcg ctgatgctgc gtgactctct gcaacgctgg
    15181 gttgccagcc agatcactgg tgaagttacc ctggagctgc gccgtgggaa cgattattca
    15241 atcctgaata ccgtctcaga gaacctgacc tacaagccag agcgtctgac gatggaaaaa
    15301 ggcgactcgg tgttctcgcc agatgatcgt attggtcaat tgaccatgcg taatctggat
    15361 atcactgata cccgagagaa actttttggt tatgccaaaa ctggcctgct ctcctcctct
    15421 gccacttcag gcgtgccgca ggtggagaat atggaaaaca aaggccagta attcgacatc
    15481 aaccctgccc ttcaggggtg gggttacctt gcttatcctc tgtaaactcc tttctgagca
    15541 tcacataatc gaaaacatca tggtttgcat ccacaacccg tgagtctcct ttatagtgtg
    15601 caatcacttc ctcaagcgtt tgcggctgtt ccggctttgt tacccccatc cacgcattaa
    15661 taaggtaatt attgtaagct gtcgaaaaga tgttgttttc cgttgtgcga tccacgccat
    15721 ccccaagaac ggggctatac cagataaaca tcgggacatg atatgcctgc tggctagcct
    15781 ccctgcctcc atgaaaatag acgttcttct tcgtagggtc acgttccagg ccgtggtcgg
    15841 caaaatacat gaccgaggcg cggcgatctt ttaataattc aaaaacctga cctagcaaac
    15901 tatctgtgta atgaatggag ttgtcatagc aggcatcctg atcgtcctgc ggttgaaaca
    15961 cggcgctgga ttgcggatag gcgctacaag ccggttcatg gcttccattt aaatgaagaa
    16021 caatcagttt cttttgctgc gtattttgct gtaatgcttg cgataaatgc ggcaacaaca
    16081 attcatcaaa tcctctgaca taaactgttt ccatggcgcg catggcgata ctggtaactg
    16141 ctgtaccatt ctgccgaaaa gcggattgcg agcttagcca gaaagtctga aatcctgctt
    16201 gattagccat attaataatg ttgtcggggt aattatgaat gtcatgactc aaaacagaat
    16261 cagcagttaa agaaagggga accgacagcg cagtgtaagg tgcgccgctt attgcttgat
    16321 taaacagttt gatctgtttt ctttgtgctt caacttgcgg tgtcgtagag cgtgtatatc
    16381 catacaaaga catattgtcg acacgtacag actcccccac aatcaacacg taggtatcaa
    16441 tacctgtatc cctgactgat aattgaaaat acggcaccgt gtttgcaatt gagagtaatc
    16501 tttgatgctc tttcgctgct aaagcaaaat agttgagatt gaaaaacggc gtataggtag
    16561 caaatcgcga cgctagtata tatggactga acgctttttt attttttgca tctttataag
    16621 caaattgaca agcggaaaat aaactgcccg aaatgacaat cagcaataat attcctgtca
    16681 ctttttttgt cggcaaggaa acatcatatt ttattattac tgccaaaaaa agaagggata
    16741 aaaaagcaaa ggcacataga taagggatat acatccccag cattttgaca acttcatccg
    16801 gatcactctg gagcacacta atcgcaaatc catcattgaa agttgtacca aaggtacacc
    16861 atgaatagag gctaatactc atgtctgccg cagtcagtaa cacaaatggt gcggcaacaa
    16921 gcaggcgaag cggaagggat gatatacgtt ttacaaccag gatggtcagg acgaagaaca
    16981 gcaatcgctg aaccatatgc gcgccggaag aggcgactaa gttcgtaata ccaaaaacaa
    17041 taacacaaag atacaacaac caatgagatt taaaacttct agcaaatttg ttgaatactg
    17101 tcatccagaa atccaagttc acaaccaata taaacaatta tcaacaatgt tcaattttat
    17161 atcagacatg tttttcaact ctgatccata tcagaccgaa acactgttag ttttttcttt
    17221 ttaaatgatg cggccattgc gttctcagta attaaataac attatcaatg cgtctctaaa
    17281 cattacgcag aacgtagcca tcagtagtta tactgacagg agcaggatgg tttatcgaga
    17341 agtaaacata tccgtattcg taccggaact ggagttgaca caataaagtg ccaattatgt
    17401 cagcagaagg gaaaagttta taactaaggc gtaccggcac catcgtttca aggtaccagc
    17461 tacgagtaaa gcaactggac gagatacaga tacctgacaa ccattcctca gaccaggacc
    17521 aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa tttggtaccg aggacgggac
    17581 ttgaacccgt aagccctatt gggcactacc acctcaaggt agcgtgtcta ccaattccac
    17641 cacctcggca cggatactac tttttagttc gggatatcgc tggtcggctt agccggagca
    17701 gctggttgag tttgttcggt tttcaccggt gcactcagat tttcccattc gctaccttta
    17761 ttggttttgt tgctattgat gttacccagc accagactga tgatgaagaa taacgttgcc
    17821 agcagcgccg tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa cagcgtagcg
    17881 gaagcgcctg ctccgaagga ggctcccata tcagcgcctt taccttgctg cagcatgatc
    17941 agaccaacaa ggccaattgc cacaataagg aaaactacta aaagagcttc gtacataatc
    18001 aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc gggagttttt
    18061 aatctttccc actgaagcgg gtgtgaatac taaccaaagc gaatgacctt cgcaagggca
    18121 attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt tcgctgagaa
    18181 aaaaggcgac aatcgccgcc tttttagcca gttatctaac gctttaaacg gcttttactg
    18241 catcggcgat gcggtgtgca aattcagtca cctgcgcttc gtcttcgcct tccaccatca
    18301 cgcgaattaa cggttcggtg ccggatttac gcagcaacac gcgtccacgg ttgcccagcg
    18361 cagcttcaac ctctgcggtc acggctttaa ctgactcatg ctcaagtgga tcgccgctac
    18421 ctgcggtgta acgtacgtta accagaatct gcgggaacat tttcatgccg ctgcaaaggt
    18481 cgtgcaggct catatggtta cgtgccatcg ccgccagcac ctgcaagcca gcaacgatgc
    18541 cgtcaccggt agtagtttta tccagtagga tcacatgacc ggaattctct gcaccgatac
    18601 gccagccttt ctcctgcatt ttttccagta cgtagcggtc gcccactttc gcgcgcgcaa
    18661 atggaattcc taactgtttc agcgccagtt caagccccat gttgctcatc aatgtaccca
    18721 cagcgccacc acgcagctgg ccctgacgaa gaccttcacg cgcgatgata tacatgatct
    18781 gatcgccatc gactttattg ccttcatggt caaccataat cacgcgatcg ccatcgccgt
    18841 cgaaggcaat accgagatcc gctttttcag ccagcacacg agcctggagc gcgcgaacgt
    18901 cggtagcccc cacttcggca ttgatgttta caccgtttgg ctcacaaccg atagcgataa
    18961 cgttcgcccc cagttcgcgc agcacgttcg gcgcgatgtg ataagtcgca ccgtttgcac
    19021 aatccaccac aatcttcagt tcactgaggc taagttcgtt cgggaacgtg gctttgcaaa
    19081 actcgatata gcgacccgcg gcatcaacga tacggctggc tttacccagt tctgccgaat
    19141 caacgcagct gatctccttt tccatttccg cttcgatggc ctcttctacc gcatccggca
    19201 gtttggtgcc gtcgatagag aagaatttaa tgccattatc gtagaacggg ttatgagatg
    19261 cagatatcac aattccggcc tctgcgcgga aggtacgcgt cagataagcc acggccggtg
    19321 ttggcatcgg gccagtgaag agtgcggaaa ggcccgctgc cgccagaccc gcttccagtg
    19381 ctgactccag catatagcca gaaatacgcg tgtctttacc aataataatc ttacgggagc
    19441 cgtggcgcgc cagcacttta cccgcggccc aacccagctt aagcacaaaa tcaggtgtga
    19501 tcggcgcatc ccctacacga ccacgaatcc catcggtacc gaaatattta cgattactca
    19561 tagcgtttgt tttcctttgc agacagagtg gcttccacca cccgcatcgc ttctacggtt
    19621 tctttgacgt catgaacacg aatgatgtgc gcgccttgca ttgcggcaat gaccgcacag
    19681 gccagactac cgctcaggcg ctcggacggc cccacgttca gcagctgccc aatcatcgat
    19741 tttcgtgaca tacccaccaa cagcggcagg ttgaaatggt gaaattcagc caggcgcgcc
    19801 agtaatgaat agttatggga gagattttta ccgaaaccga atccggggtc gagcaacaat
    19861 ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa gtagcgattc
    19921 acttctgcaa agacatcgtc atacttcgga gcttcctgca tggtttttgg atttccctgc
    19981 atatgcatca gacaaaccgg taaaccggtt tctgcagccg cctccagagc gccaggttcg
    20041 gaaagggagc ggatatcatt aataatgtga gcgccaactt tcgctgactc acggatgact
    20101 tctggtttgg atgtatcgac cgagatccag acttcgaagc gttgagcaat tgcctcaacc
    20161 acaggaataa cacgttgcaa ctcttcttca acgctaactt ccgccgcccc tgggcgcgtg
    20221 gactcgccac caacgtcaat gatcgtcgcg ccagcattga tcatcagatt cgcatgtttc
    20281 accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa aggaatcagg cgtgacgttg
    20341 aggatcccca ttacgtgagg atggctaagg tccagtgaag taccctgggc aaagagtttc
    20401 atgatgttat ccctagtatg aattgataag aaaaaacccc ggagcacgcc ccggggtttt
    20461 cggtacaaat acagtcatct gatgcgggaa cttacttgtc gcctaactgc tctgacatgg
    20521 tgttacccgg gttcggcgta cgcggttcat caaccggacg aggagccttt ggactaccat
    20581 tgtcgccaga attgttagaa gcgcctggtt cttcccagcc cgctggcgga cgtacatcgc
    20641 gacgtgccat caggtcatca atctgcggtg cgtcgatagt ctcatatttc atgagagcat
    20701 ctttcatcgc atgcagaata tccatattgt cggtcagaag ctgacgcgca cgattatagt
    20761 tacgctcaat cagtgctttc acttcctggt cgatgatacg tgcagtttca tcggacatat
    20821 gtttcgcttt cgctacgcta cggccgagga atacttcacc ttcttcttcc gcgtacagca
    20881 gtggacccaa tttctcagag aagccccact gggtcaccat gttacgtgcc agattggtcg
    20941 caactttaat atcgttggac gcaccggtag aaacatgttc cggcccatag atgatctctt
    21001 ctgccagacg accaccgtac agcgtagaaa tctggctttc cagtttctga cggctggcgc
    21061 tgattgcgtc gccttcaggc aagaagaaag tcacacccag cgcacgaccg cgtgggataa
    21121 tcgtcacttt gtgcaccgga tcgtgttccg gcaccaggcg accgataatc gcatgacccg
    21181 cttcgtggta agccgttgat tctttctgcg cttccgtcat caccatggag cgacgttccg
    21241 cacccatcat gattttgtct ttcgctttct cgaactcaac catcgacaca acgcgtttgt
    21301 tgccacgagc agcgaacagt gccgcttcgt tcaccaggtt cgccaggtca gcaccggaga
    21361 aaccaggagt accacgggca atgattgccg cgtcgatatc gggggccaat ggtacgcgac
    21421 gcatgtgaac tttcaggatc tgctcacgac cgcgaacatc tggcaagccg accacaacct
    21481 gacggtcgaa acggccagga cgcagcaggg ccgggtcgag aacgtccgga cggttagtcg
    21541 cggcgataac gatgatacct tcgttacctt cgaagccatc catctcaacc agcatctggt
    21601 tcagagtctg ttcacgttca tcgtgaccac cgcccagacc agcgccacgc tggcggccta
    21661 cggcgtcgat ttcatcgata aagatgatgc acggtgccgc tttcttcgcc tgttcgaaca
    21721 tgtcacgaac acgggatgca cccacaccga cgaacatttc tacgaagtca gaaccggaga
    21781 tagtaaagaa cggaactttc gcttcgcctg caatcgcttt cgccagcaac gttttaccgg
    21841 tacccggagg accgaccatc aggacgcctt tcgggatctt accgccgagt ttctggaagc
    21901 ggctcggctc gcgcagatac tctaccagtt cagcaacttc ttcttttgct tcgtcgcagc
    21961 ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt cagcatgcgc gctttgctct
    22021 taccaaacga catggcacct ttgccaccgc cgccctgcat ttgacgcatg aagaagatcc
    22081 agacaccaat cagcaacagc atcgggaacc aggagatgaa gatagaagcc agcaggcttg
    22141 gttcttcagg cggttcaccg acaaccttga cgttcttggt caacaggtta tccagtaatt
    22201 tcggatcctg aaccggaatg taagtggtat aacggttact atctttcttg gtaacgttga
    22261 tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt attgacctct tgtaggaagg
    22321 tagagtaatc caccttacgg ccattagact cgctgggccc aaagctctgg aatactgaca
    22381 tcagcacaac ggcaatgacc agccagagta ttaggttttt cgccatgtca ctcaagggat
    22441 taacctctta ttacaactgt gttaaaaaca gcgtcaggat actctatatc cagcatcttt
    22501 caaactttcg tctgaaatct cccggttagg gtttacgccc ggtcgctaca atatacactt
    22561 cccgcgaacg tgcacgagaa gagtccggct tacgaacttt gaccttcgta aacagggagc
    22621 gaatttccct tagatactca tcgaaacctt cgccctggaa caccttcact acaaaactgc
    22681 cacctggcgc taatacatca cgacacattt ctagcgccag ttccaccaga tacatggcac
    22741 gggggatatc caccgccggt gttccgctca tgtttggtgc catatcggac atgacaacct
    22801 ggactttgct gtcgccaacg cgctccagca gtgctttcat caccagttca tcacgaaaat
    22861 cgccctgaag aaagtccaca ccaacgatag gatccatagg taaaagatcg caagcgatga
    22921 tgcggccttt gccgccaatt tgggtgacca catattgtga ccaaccaccc ggagcagcac
    22981 caaggtcgac aaccgtcatt cccggtttaa agagtttgtc actttgctgt atttcatcaa
    23041 gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc ctgttgaaca tatttatcgc
    23101 taaagtgttc ctgaagccag cggctggagc tggcagaacg cttcttacct gtcatttaac
    23161 tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc ctatttggtg
    23221 atatatggga gatggcggta gaatgacccg ttttcaatcc caacgtaagc aaaaaatacg
    23281 atgaatctga gtactaaaca aaaacagcac ctgaaaggtc tggcacatcc gctcaagcca
    23341 gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc tggccgagat tgaacaagcg
    23401 ttagagcacc atgaactcat caaggtgaaa atcgccaccg aagatcgcga aactaaaacc
    23461 ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta atgtacaggt catcggtaaa
    23521 acgctggtgc tttatcgccc aactaaagaa cgtaaaatct cgctgccacg ctaagattat
    23581 cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt tccgcaggca ggagagcaaa
    23641 atgccacgct ctgttcgttg ataaaaggcc gcatagcggc ctttttcctt tctttacaat
    23701 acatcaacat cttgagtatt gggtaattct tacaggtatt ccaccttaat tacttcaaat
    23761 tctacttcgc cgcccggcgt tttgatgacc acaacatcat cttcttcttt gccgatcagg
    23821 ccacgagcaa taggcgagtt tacagaaatc aggttttgtt taaagtcagc ttcgtcatcg
    23881 ccaacgatgc gataagtctg ttcttcgtca gaatccagat tcagcaccgt tacggtagca
    23941 ccgaaaataa cgcgcccatt gttgggcatt ttggtgacat caatcacctg cgcgttcgac
    24001 agcttggctt cgatgtcttt aatacggcct tcgcagaaac cctgctgttc acgagctgcg
    24061 tggtattcgg cgttttcttt caggtcgcca tgctcacgcg cttccgcgat agcagcaatg
    24121 atttcaggac ggcgcacaga tttcagaaaa tccagctctt cgcgtaattt ttcagcgccg
    24181 cgtaaggtca tcggaatagc ttgcatttgt tatacctctt gaatattcct gatggggcaa
    24241 gtcttcaccc accccggctg ttcggcctgc ccggcataac tcctcaccgg gaccagaagc
    24301 aaaaaaatac cgacccgggt acaagtccca ggtcagctac aattcacatt ttgatagtca
    24361 ttttaccctg aagttcccga agggtcatcg tttactttat agggcgttgc gccgtagtat
    24421 gacggctcga ttccaggttg ttagcgcgag attatgcgat tttccagatt tatcatcgga
    24481 ttgaccagct gtatagcgtt cagtgttcag gccgcgaatg ttgatgagta cgttactcaa
    24541 ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag tcggcgcgtc ggcccccgct
    24601 attgattacc acagtcagca gatggcgctg cccgccagta cgcagaaagt gattactgcg
    24661 ctggcggcgt tgattcaact cggccccgat tttcgtttca ccacgacgct tgaaaccaaa
    24721 ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg cgcgatttgg tgccgatccg
    24781 acgttaaaac gtcaggatat tcgcaatatg gtcgcgactt tgaaaaaatc tggcgtcaac
    24841 caaatcgatg gcaatgtgtt gatagatacc tccattttcg ccagccacga taaagccccc
    24901 ggctggccat ggaatgacat gacacaatgc tttagcgctc cgcctgccgc tgccatagtt
    24961 gaccgcaact gtttctccgt ctcgctctac agtgccccaa aacctggtga tatggctttt
    25021 atacgcgtgg catcttatta ccccgttacg atgttcagcc aggtacgcac cctcccccga
    25081 ggttctgccg aagcgcaata ctgcgaactg gatgtcgtac caggcgacct gaaccgcttt
    25141 acgctgacgg gatgcctgcc acaacgttct gagccgctcc cgttggcttt tgccgtgcag
    25201 gatggagcca gctatgccgg ggcaattctg aaagatgagt taaaacaggc aggtatcacc
    25261 tggagcggaa cactgctgcg ccagactcag gttaacgaac ctggaacggt agttgccagt
    25321 aaacagtcgg ccccgttgca cgatctgctt aagattatgc tgaaaaagtc ggacaacatg
    25381 atcgccgata cggttttccg catgataggc catgcgcgct tcaatgtgcc tggaacatgg
    25441 cgggccgggt cggacgccgt gcgtcagatc ctgcgccagc aagccggtgt cgatattgga
    25501 aacaccatta ttgccgatgg ttcagggctt tcgcggcata acctgattgc ccccgccacc
    25561 atgatgcagg tgctgcaata cattgcccaa cacgacaatg aacttaactt tatctccatg
    25621 ctgccgcttg cgggctatga cggctctttg cagtaccgtg caggtctgca tcaggcgggc
    25681 gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg gggtatataa cctggcggga
    25741 ttcattacca cagcgagcgg gcaacgaatg gcgtttgtac aatatctttc tggctatgca
    25801 gtagaaccgg cggatcagcg taatcgccgt attccgttag tgcgttttga aagccgtttg
    25861 tataaagata tttatcagaa caattagtca aaaagaaacc ccggcacatg gccggggttt
    25921 tgctgattaa cgcttgtaaa tgaactcaac gccttcttcg tcgtcttcgt cccagtcatc
    25981 atcccagtcc tcatcatcct cttcagcaat ctcttcaagc tgctggcgat gataatcatc
    26041 ccacatgaat tcgactttct ctggctgttt cgcttcttca gcctgcacga ccgggttttc
    26101 aatgataaag gtcatcacat cccagcagag atctttcacg cccagtccac tcgccgcaga
    26161 gatcagataa tatttatctt cccagcccag cgcttcagca atcgctttcg ctttctcttc
    26221 agcttctgcc ttatccagca gatcgatctt attgaacact aaccaacgcg gtttcgccgc
    26281 cagatcctgg ctgtattttt ccagctcgct gataataata cgcgcgtttt caaccggatc
    26341 ggtgccgtca atcggatcga tatcgatgag gtgcaacagg acgcggcaac gttccaggtg
    26401 cttcaggaag cggataccca gacctgcgcc ttccgcagcg ccttcaatca gtcctggaat
    26461 atcggcaaca acgaagctct tttcgttgtc cattcgtacc acacccagac ttggcaccag
    26521 agtggtaaac ggataatccg ccactttcgg tttagccgcc gataccgcgc gaataaaggt
    26581 cgatttaccc gcgtttggca tccccaacat accgacgtca gccagcagca tcagctccag
    26641 cagcagctca cgcttatcac ccggcgtacc gttggttttc tgccgtggtg tacggttaac
    26701 ggacgatttg aaacgggtat tgcccagacc gtgccagccg cccttagcaa ccagcagacg
    26761 ctgaccgtgt ttagtcatat cgcccatggt ttcaccagta ccttggtcga ttatacgcgt
    26821 acctaccggc actttaatcg tcacgtcttt accgcgctta ccggtacaat cgcggcttgc
    26881 gccgttctga ccgcgctctg cacggaaaga tttttcaaaa cgataatcga taagcgtgtt
    26941 caggttttcg tcggcttcca tccatacgtc accaccatca ccaccgtcgc cgccatccgg
    27001 gccgcctttc ggaatatact tttcgcggcg gaagctcacg caaccattac cgccatcacc
    27061 tgcaacgacc agaatcgatg cttcatcaac aaacttcatt ttattctccg taaatcattc
    27121 gcctgcgcgg ggttgcgata ccaccgttgt atgcttacgt aatccgcccc aaatacgatg
    27181 accaatggcg gaatacatcg cgcccgcaac cacgacaaac gcaccgagat aacctaaaag
    27241 gtttaacatc ggtctggcga agaaatcggg ccaggccagt gataaaagat ctgaaaaaaa
    27301 cagcgtaaac agtggggtga gcgtgatgat cgcgctcacc tgcgctgcct gccagcgagc
    27361 catcgcttcc gccagggcgc catatcctac caatgtattc agtccgcaaa aaattaaaca
    27421 tgcgagctgc cagtggctaa gctgcgctat cactccaggc tttgccagag ggaagagcgc
    27481 aattgtacat aaagcgtaca gtaaaaacag gatctgcggt gaggccagcc gacgcaataa
    27541 aaccttttgc gccacgccat aactcaccca aaccgtcgcc gcaccgaccc caaagataac
    27601 tccccaggtg taatcggtga gctttgtaaa tatctcgacc agactggtgt taaaaaacat
    27661 caccaggccg ctcaggagca tcaatgcccc tacaacctga gtgctgcgca ttttctcttt
    27721 caggataaat acgctggcaa ccatcatgcc aactggcgag agttgcccaa tcacctgcga
    27781 agcggtcgga ctcaggtatt gcaaggatga gctgaacagg atgaagttcc caaacagccc
    27841 ggcggtcgcc actgccaaca aaatcaacca gcgtggctta cgaaacacgc gtaatggcgg
    27901 caacctcttc ttcaccgcaa gaatggcacc aaggccaata ctcgccatca agaaacggta
    27961 aaacacgatt gtcggaggtt ccatcacctc cagcacctgc ttcattgcga ttggcaacgc
    28021 cccccagcaa attgctgtgg tgagcgccaa aagaatgcca atgcctgcct gctgcttcat
    28081 accgtattcc ctgcaaagcg catttgcgga ttaacgtcgc accatgcgca tttttaccgg
    28141 ttatcgaatg taaaaagccc cgcaacgtgt tgcggggctt tcatccgtta ccgggacgcg
    28201 aaaaacttat tcagcttcga tgctgataaa tttacggttt ttcgggcctt taacttcgaa
    28261 tttcacttta ccgtctgctt tagcaaacag agtgtggtca cgaccgcaac ctacgttagc
    28321 gccagcgtgg aatttggtac cacgttgacg aacgatgatg ctacccgcca gaacggattc
    28381 gccaccgaaa cgcttaacgc ccaggcgttt agcttctgaa tcgcgaccgt tacgtgtgga
    28441 gccgccagcc tttttatgtg ccatttgaaa tctctcctca ggtcttaggc gctgatgcca
    28501 gtaattttca catcagtgaa ccactgacga tggccctgct gcttacgata gtgtttacga
    28561 cgacgaaact taacgatttt aactttctcg ccacgaccgt gagcaacaac ttcagctttg
    28621 attacgccgc catcaacgaa aggaacgccg attttgactt cttcaccgtt tgcgatcatc
    28681 agcacttcag cgaactcaac agtttcgcca gttgcgatgt ccagcttttc caggcgaacg
    28741 gtctgacctt cgcttactcg gtgttgttta ccaccacttt ggaaaaccgc gtacatataa
    28801 aactccgctt ccgcgcacac cttttcaatg attcagagtg cgctataaat attcacaata
    28861 gggcgcgaat attacgcaaa acgcacgcct ttgacaagtg ctacagtcaa tacacgaaga
    28921 aaaaaaacac aacttgtacg gtaacgttta tctgtgccat tttttcagta caatcactct
    28981 atattcctaa ccataaaccc taagttgcct ttgttcacag taaggtaatc ggggcgaaaa
    29041 gcccggcttt tgcgatgaat ttagaaaaaa tcaatgagtt aaccgcgcaa gatatggcgg
    29101 gtgttaatgc ggcaatcctt gagcagctta attccgacgt ccagctgatc aatcagttag
    29161 gctattacat cgtcagcggc ggcggtaaac gtattcgtcc gatgattgct gtactggctg
    29221 cacgagctgt tggctatgag ggaaatgcgc atgtcaccat tgctgccctg atcgagttta
    29281 tccacacggc gactctgctg cacgacgacg ttgtggatga atcagatatg cgcaggggta
    29341 aagctaccgc caacgctgca tttggcaatg ccgccagcgt gctggtaggc gattttattt
    29401 atacccgcgc gttccagatg atgaccagcc tcggttcgct caaagtgctg gaagtcatgt
    29461 cagaagccgt aaacgtcatc gcagaaggtg aagttctgca actgatgaac gttaacgatc
    29521 cggacatcac tgaagaaaac tacatgcgcg ttatctatag caaaaccgcg cgtctgtttg
    29581 aggctgccgc gcagtgttcc gggattctgg ctggctgtac gccggaagag gagaaaggcc
    29641 tgcaggatta tgggcgctat ctcggcactg ctttccagtt gatcgacgat ttactcgatt
    29701 acaatgccga tggcgaacag ttaggtaaaa atgtcggcga cgatctgaac gaaggtaaac
    29761 cgacgctgcc gctgctgcat gcgatgcatc atggcacacc agaacaggca cagatgatcc
    29821 gtaccgccat cgaacagggt aacggtcgcc atcttctgga accggttctg gaagcaatga
    29881 acgcttgtgg atctcttgaa tggacgcgtc agcgtgccga ggaagaagca gacaaagcca
    29941 tcgcagcgtt acaggtgctc ccggacaccc cttggcgaga agcactcatc ggcctcgcgc
    30001 acatcgctgt tcaacgcgat cgttaatccc ctcccctcat cccgcgcagc gcgcggggtg
    30061 gttccagtaa attccataaa aacacttact cagctctata acacctgaaa aacaggctgt
    30121 gcatctttat atattctgaa tattcacact ccttacagga actttttaga gcaatcggcc
    30181 atcaggagta tagtgatgct cgacagaaga agtgttctga atgaaagcga ctaacttaag
    30241 gagtgaggaa agatggaaag taatttcatt gactggcatc ccgctgacat cattgcgggt
    30301 ttgcgcaaga agggaacgtc aatggcggcg gagtctcgca gaaatggttt gagttcctca
    30361 acgctggcga atgcattatc gcgcccatgg ccgaaaggag agatgattat tgcgaaagcc
    30421 ctgggaactg acccctgggt tatctggcca tcacgctacc atgatccgca gacccatgag
    30481 tttatcgaca gaacgcagtt gatgcgtagc tacactaaac cgaaaaaatg agtggtctgg
    30541 cggtagcccc gcgaacgggg ctgccagctc tcagacgatt actcgccttt cacacgctca
    30601 atatttgcac ctaaagcgcg cagtttgtct tcaatgcgtt cgtagccacg atcgatgtga
    30661 taaatacgat caaccaccgt cgtcccttcc gcaatacagc cagccagcac caggcttgct
    30721 gatgcacgca gatcggttgc cataacctgt gcgccagaaa gtttttcaac accgtgacaa
    30781 ataacggtat tgctttcgat ttcggcatgc gcgcccatac ggctaagctc cggcacatgc
    30841 ataaagcggt tttcaaagac cgtttcggtg atgaatccgg tcccttctgc caccaggttc
    30901 aacagcgtga actgggcctg catatcggtc gggaatgccg gatgcggcgc ggtacgtacg
    30961 ttaacagcct tcggacgttt gccatgcata tccaggctaa tccagtcttc gccgacttcg
    31021 atgtccgctc cagcgtcacg cagtttcgcc agcacggcgt cgagagtatc tggctgcgcg
    31081 ttacggcaga taattttgcc gcgagaaatc gccgccgcca ccaggaaagt accggtttcg
    31141 atacgatccg gtagcacgcg atagacaccg ccgcctaaac gttccacacc ttcgatgacg
    31201 atacgatcgg tgccctgacc gctaattttc gcacccagcg taatcaggaa gttcgcggta
    31261 tcgacgattt ccggttcacg cgctgcgttt tcaataatcg tggtaccttc cgccagggtt
    31321 gcagcacaca tgatggtcac cgttgcgcca acgctgactt tatccatcac gatatgtgcg
    31381 cctttcagac gaccatcgac ggaagcttta acgtaacctt cttccagttt gatggtcgcg
    31441 cctaattgtt cgaggccaga aatgtgtaga tcaaccggac gcgcaccgat cgtacaaccg
    31501 ccaggcagtg aaacttgccc ctgaccaaag cgcgctacca gcggccccag tgcccagata
    31561 gaagcacgca tggttttaac cagatcgtaa ggtgcgcaga atacattaac gtcgcgggca
    31621 tcaatatgca cagaaccatt acgttctact ttcgcaccca gctggcttag cagcttcatt
    31681 gatgtatcga cgtctttcag tttcgggacg ttctggatct ctaccggttc ttccgccagt
    31741 agagcggcaa aaaggatagg cagagcagca tttttagcgc cggaaattgt gacttcgccc
    31801 tggagcttcg ttggcccctg aacacgaaat ttatccattt agtttgttct cagttaacaa
    31861 ttcatatccg ctaccggcga atcgcccata gctcaaaagc cgttcagttt gcgatcgcgc
    31921 gcccactccg caggggtata cgctttgatc gacacagcat gaatgcggtt atccgcaata
    31981 tattccatca gcgggccata gaccgtctgc tgttttttaa cccgactcat gccgtcaaac
    32041 aactcaccca cggcaataac ctgaaagtgg ctgccatcgc cggaaacgtg gacttcctgg
    32101 agggagagag cgttcatcaa cacgctctga atttcattat tttccatggg atcttcaatc
    32161 atcagttaat aaaccagcga aacatcttag agcaaagttg cgctggcata aataagcaaa
    32221 aagcctcgct gataaatcag acaaggctcg acttgcaggc aggtttgccg gacaggcggt
    32281 taacgccata tccggcctga aaaaatttaa cgaggcagaa catcagcagg caaattatac
    32341 aatttcgcga gggtatacac tttgtcgttt accccctgaa gcgtcacgtt gttgccctgc
    32401 tttttcgcca gatcgataag atggagcagc agtgccagtc cacccgtatc cacgcgggac
    32461 acacggctaa gatcgatgca ggtaatcccc ttcaccgctt cctcacgcat ttcccaaagc
    32521 ggtagcagaa cgtcctgatc cagctctccg gataacgcca gcgtgtcacc cgtctgcatc
    32581 cagctcagtg actcgctcat tattttttct cttccagagt gattttctgt tgagaaatcg
    32641 atttcagttg cgcagtcagg ccgtcgatac ctttggtacg cagcagcgtt ccccactcgt
    32701 tttgtttggt ggtgatcata ctgacgcctt cagcaatcat gtcgtaagcc tgccagtttc
    32761 ccgtctggga gtttttacgc cactggaagt ccagacgcac cggcggacgg ccattcgggt
    32821 caataatggt aacgcgaata ggcacaatgg ttttatcgcc cagcggctgt tctggcgcaa
    32881 tctgataggt ttgaccgtga tacatcgcca gcgcctgacc gtaagcctgc ttcaggtact
    32941 cacggaaagc ggcaaagtag gcttcacgtt gagcaggggt cgcactcttg taatactggc
    33001 ccagcaccag cgcaccggcg tatttcacct gtacgtatgg cagcagttcc tgatcaacaa
    33061 tggtacgcag ataatccggg ttggcccgaa tttgcggttg ctcattcttc aggcgatcga
    33121 acgttttctg cgccgcctcg tccatcagct tatacggatt ggtctggtct gccgcggttg
    33181 ccgcactcag aggtgcaatc accagcaaag cgaccatcat taaacgttta aacatgcgtc
    33241 ggttctcctg aaattatttc gttgtaccca caggttcagt ggtttcatta ttacctggcg
    33301 cagcagctgg cgcatcgcca ctattcttat tgtcatcgcc tttactaccg taaaggaact
    33361 gaccaatgag atcttccagc accatcgcag acttagtgtc ctgaattgta tcgccatcct
    33421 tcaggatagc agtccccagt tccgggtctt caaaaccgac gtttaatgcc agatattgtt
    33481 cccccagcag gccggaagta cgaatgctca gcgaactggt atctggaatg tggttataac
    33541 gttgttcaat ttccagcgtt acgcgtggca gataggtttt cgggtccagc gtaatatccg
    33601 ccacccggcc cacaacaacg ccaccaatac tgaccggaga gcgcgctttc aggccgccaa
    33661 tgttatcgaa cgtcgcataa agcgtgtagg tcggttcagt acgtatggac gtcacgttcg
    33721 ccgccttcag gcaaacaaac agcgccgcca gcagtgctgc taataaaaag atacccaccc
    33781 aaatttcatt ttttttcgtt tgcatgaact caattcccaa acatcaatgc ggtcagcaca
    33841 aaatccagcc ccagaacagc cagagacgag tggacaacgg tgcgagtggt tgcccggcta
    33901 atcccggcag acgtcgggat ggcgtcgtag ccgttaaaca acgaaatcca cgtcaccgtg
    33961 atggcgaaca ccacgctctt aatcagacag ttgaccagat ccatacgcca gtcgacggca
    34021 ttttgcattg ccgaccagaa gaacccgcta tcaatgcctt tccaactgac gccgaccaga
    34081 gatccgcccc agatccccac ggcgacgaaa ataaccgtca acagtggtaa tgaaataacc
    34141 ccagcccaga aacggggaga aataacccga cgcagcggat ccaccgccat catctccata
    34201 ctggagagtt gctctgtagc gcgcatcagg ccgatttctg cggttagcgc cgaaccagca
    34261 cgcccggcaa acaacaacgc ggcaaccacc ggccccagtt cacgcagtag cgataacgcc
    34321 accagcatac ccagactggt ttccgcacta taagtggtca gaaccagata accttgcagc
    34381 cccaacacca ttccgatgaa cacgccagaa accacaataa tcagcatcga caggacgccg
    34441 acattataga gctggcgcac cagcagcggc gcatgtttgc gaaattccgg tttgccgacc
    34501 agcgcattga ataacattaa cccggcccgc ccgaacgttc tcagggtttt aatcccttta
    34561 tgtccgagcg acgccagcgc atttaacagc atgagtggct taactccctg gtaaaagatc
    34621 agcgtgataa tcgccggcag gatagcggaa cggaacaggc ccgtcagcta tcccgtccag
    34681 aaactgacgt acgcgcggat caggattcgc ctgcaacgcc tgggcactgc catgagcgac
    34741 aatttttttg tccgccagga tccaggcgtg atccgcaata cttaacactt ccggcacatc
    34801 gtgagaaacc accacacaag tcacgcccag cgcgctgttc agctcagaaa tcagcttcac
    34861 cagtacgccc atggtgatgg gatcttgccc aacaaaaggt tcatcaaaca tgatgagatc
    34921 cggctccagc gcaatcgcac gcgccagcgc tgcacgccgc gccatcccac cagaaagttc
    34981 agaaggcatt agcttagccg ctccacgcag ccccacggcc tccagcttca tcatcactgt
    35041 actatgcaac aatggcgcgg gaagttgggt atgttcgcgc agtggatagg cgacgttgtc
    35101 aaatacgttc atatcagtga acaacgcccc ggactgaaat aacatgctca tccgtttgcg
    35161 cactgtatac aggcgcgaac gagacatcgc cggaatattc tcaccatcga aaaggatctc
    35221 accatgatct ggtgcgattt gcccgccaat cagacggagt agcgtcgttt taccgatgcc
    35281 cgatggcccc atgattgccg tgatcttccc tcgcggcacg gtcagggaaa tattatcgaa
    35341 gatgcagcga ttgccacgcg taaaactgac atcgcgcata tcgactaaat tcgccacaga
    35401 ctgctccata attcaccctt cgtcttgcgt tgattttcta agcatggcgc tcaatttaac
    35461 cttgaaccca acatatttac agaatattac ccgccgtggt tagcgaaagc tggcatttgt
    35521 tttacttttt agccgcataa agtcaaaatt aagaatccgt tacggctttc tgaaaatctt
    35581 cagcggaccg gcgagtatac ctgaagaaag gatgttagat gcttttagct acggcactgt
    35641 taattgttgg tttacttttg gtcgtttaca gtgccgaccg cctggttttt gccgcgtcta
    35701 ttctttgccg aacctttggc atcccgccgc tgatcatcgg catgacggtg gtcagtattg
    35761 gtacatcgtt accagaagtc atcgtctcgc ttgccgcgtc tctgcacgaa caacgcgatt
    35821 tagccgttgg tacagccctc ggctcaaaca ttatcaatat attgctgatc ctcggtctgg
    35881 ctgcgctggt tcgtcctttt accgtccatt ctgatgttct acgccgtgaa ttacccttaa
    35941 tgttgttggt cagcgtggtg gccggttccg tactctatga cggacaactt agtcgcagcg
    36001 atggtatctt tctcctcttt ctggctgtgc tatggctgct gttcattgtt aaacttgcac
    36061 gtcaggctga acgtcagggg actgacagcc tgaccagaga gcagcttgca gagctgccgc
    36121 gtgacggcgg attgcccgtc gcgtttttat ggctcggcat tgcgcttatc atcatgccag
    36181 tggccacgcg gatggtggtt gataacgcca cggtgctggc gaattacttt gccatcagcg
    36241 agttgacgat gggtctgacg gcaattgcta tcggaaccag cctgccggaa ctggcaaccg
    36301 caatagcggg ggttcgcaaa ggtgaaaacg acattgctgt cggaaatatc attggcgcaa
    36361 acatttttaa tattgtcatc gtgttgggtt tacccgcgct gataacgcca ggagagattg
    36421 atccactggc gtacagtcgt gactacagcg tgatgttgct ggtgagcatt atttttgcgt
    36481 tgctgtgctg gcggcgctcc ccacaaccgg gccgtggtgt aggggtatta ttaactggcg
    36541 gatttatcgt atggctggcg atgttgtact ggttatcgcc aatactcgtt gaataactgg
    36601 aaacgcatta tgtcgcacgt agagttacaa ccgggttttg actttcagca agcaggtaaa
    36661 gaagtcctgg cgattgaacg tgaatgcctg gcggagcttg atcaatacat caatcagaat
    36721 ttcacgcttg cctgtgaaaa gatgttctgg tgtaaaggga aagttgtcgt catggggatg
    36781 gggaaatcgg ggcacatcgg gcgcaaaatg gccgcaacgt ttgccagcac cggtacacct
    36841 tcatttttcg tccatcctgg tgaagccgcg catggtgatt taggcatggt caccccacag
    36901 gatgtggtga ttgctatctc taactcaggt gaatccagcg aaatcacggc cttaattcca
    36961 gtgcttaagc gtcttcacgt accgttaatc tgcatcaccg gtcgcccgga gagcagcatg
    37021 gcgcgcgctg cagatgtgca tctgtgtgtt aaagtagcga aagaagcctg tccgttaggg
    37081 ctggcaccga ccagcagcac caccgccacg ctggttatgg gcgatgccct cgctgtcgcg
    37141 ctgttaaaag cacgcggctt tactgctgaa gattttgcgc tctcacaccc aggcggcgca
    37201 ctgggtcgta aacttctgct gcgcgtaaac gatattatgc atacgggcga tgagatcccg
    37261 catgttaaga aaacggccag tctgcgtgac gcattgctgg aagttacccg caaaaatctt
    37321 ggtatgactg tcatttgcga tgacaatatg atgattgaag gcatctttac cgacggtgat
    37381 ttacgccgtg tcttcgatat gggcgtggat gttcgtcagt taagtattgc cgatgtgatg
    37441 acgccggggg gaatacgtgt gcgccctggc attctggccg ttgaggcact gaacttaatg
    37501 cagtcccgcc atatcacctc cgtgatggtt gccgatggcg accatttact cggtgtgtta
    37561 catatgcatg atttactgcg tgcaggcgta gtgtaaagat tcaaggataa acaacaatga
    37621 gcaaagcagg tgcgtcgctt gcgacctgtt acggccctgt cagcgccgac gttatggcaa
    37681 aagcagagaa cattcgtctg ctgatcctcg acgttgatgg cgtactgtca gatggcctga
    37741 tttatatggg caataacggc gaagagctga aagcgttcaa tgttcgtgac ggttatggca
    37801 ttcgttgtgt gctcacctct gatattgaag tcgctatcat taccgggcga aaggctaaac
    37861 tggtagaaga tcgttgtgcc acattgggga tcactcactt gtatcagggg cagtcaaaca
    37921 aactgatcgc ctttagcgat ttgctggaaa aactggcgat tgccccggaa aatgtggctt
    37981 atgtcggcga tgatctcatc gactggccgg taatggaaaa agtgggttta agcgtcgccg
    38041 tggccgatgc gcatccactg ttgatcccgc gcgccgatta cgtgacgcgc attgctggcg
    38101 gtcgtggcgc agtgcgcgaa gtttgcgact tattactcct ggcgcagggc aaactggatg
    38161 aagccaaagg gcaatcgata tgagtaaagc cagacgttgg gttatcattg tgctatcact
    38221 ggcggttctg gtgatgatcg gcattaatat ggccgaaaaa gacgataccg cccaggtggt
    38281 cgtcaacaac aatgatccca cctataaaag cgagcatacg gacacgctcg tctataaccc
    38341 agaaggggca ctaagctatc gattgattgc tcaacacgtt gaatattatt ccgatcaggc
    38401 cgtttcgtgg tttacgcagc cggtacttac cacgtttgat aaggataaaa tcccgacatg
    38461 gtccgtaaaa gcagataaag ccaagctgac caatgaccgg atgctctatt tatatggaca
    38521 cgttgaagtc aacgcactcg tgccggactc tcaacttcgc agaatcacga cggataacgc
    38581 gcagatcaat ctggtgacgc aggatgttac ctctgaagac ctcgtcacgt tatacggaac
    38641 aacatttaac tccagcggtc tgaaaatgcg cggcaactta cgcagcaaga acgccgagct
    38701 gattgaaaag gttagaacat cctatgaaat tcaaaacaaa caaactcagc cttaatcttg
    38761 tgcttgccag ctcacttctg gccgccagca ttccggcatt tgccgtaacc ggagacactg
    38821 atcagccgat ccacattgaa tcggaccagc aatctcttga tatgcaaggc aacgtggtta
    38881 cctttaccgg taatgtcatc gtcacccagg gcaccatcaa aattaatgcc gacaaagtgg
    38941 ttgttacccg tccgggcggc gaacaaggta aagaagtgat tgacggctac ggtaaaccgg
    39001 caacgttcta ccagatgcag gacaacggta aaccggttga aggtcacgct tcccagatgc
    39061 actacgaact ggcaaaagat tttgtcgttc tgacgggtaa tgcttatctg cagcaggttg
    39121 atagcaacat taagggcgat aagatcactt acctggtaaa agagcagaaa atgcaggctt
    39181 tcagcgacaa aggcaagcgc gtaaccaccg ttctggtgcc gtcgcagctg caggacaaaa
    39241 acaacaaagg ccagaccccg gcacagaaga agggtaatta attcgttatg gcaacattaa
    39301 ctgcaaagaa ccttgcaaaa gcctataaag gccgtcgcgt ggtagaagac gtcagcctga
    39361 ccgtcaactc cggggaaatt gtcggtctgc tggggccaaa cggtgccggt aagaccacca
    39421 ctttctatat ggttgtaggc attgtgccgc gcgatgcggg caacatcatt attgatgatg
    39481 acgatatcag tctgctgcct ctgcatgcac gcgcgcgccg cggtatcggc tatctgccac
    39541 aggaagcctc cattttccgt cgcctcagcg tttacgataa cctgatggcg gtactgcaaa
    39601 ttcgtgacga cttgtctgct gaacaacgtg aagaccgcgc gaacgagctg atggaagagt
    39661 ttcacattga gcacctgcgt gacagcatgg ggcagtcact ctccgggggt gaacgtcgcc
    39721 gtgtagaaat tgcccgcgca ctggctgcga atccgaaatt tattctgctc gacgaaccgt
    39781 ttgccggtgt tgacccgatc tcggttatcg acattaaacg catcattgag cacctgcgcg
    39841 acagcggcct gggcgtgctg atcactgacc acaacgtgcg tgaaacactg gcggtttgtg
    39901 aacgcgctta tatcgtcagt caggggcatt tgatcgccca cggcacgcct acagaaatct
    39961 tacaagacga acacgttaag cgtgtatacc ttggggaaga cttcagactc tgatagggta
    40021 gaagtttgcg acgttttagc aggagagtac gattctgaac atgaagcaag gtttgcaact
    40081 caggcttagc caacaactgg cgatgacgcc acagctccaa caggcaattc gtctgttgca
    40141 gttgtcgacg ctggaacttc agcaggagct acagcaggcg ctggagagta atccgctgct
    40201 tgagcaaatc gacactcatg aagaaatcga cacccgcgaa acgcaagaca gtgaaacgct
    40261 ggacaccgcc gatgcgctcg aacaaaaaga gatgccggaa gagctgccgc tcgatgccag
    40321 ttgggacacc atttacaccg ctggtacacc atccggcacc agcggtgact acattgacga
    40381 tgagctgccg gtctatcagg gcgaaacgac gcagaccttg caggattacc tgatgtggca
    40441 ggtcgagctg acaccgtttt ccgacactga ccgcgctatt gctacctcta tcgtcgatgc
    40501 cgttgatgac accggttatc tgactgtccc gctggaagat attctcgaaa gtatgggcga
    40561 tgaagagatc gacatcgacg aggttgaagc cgtccttaag cggatccaac ggtttgatcc
    40621 ggtcggtgta gcggcaaaag atctgcgtga ctgcctgcta atccaactct cccaattcga
    40681 taaaaccacg ccgtggctgg aagaggccag attgatcatt agcgatcatc tcgatctgtt
    40741 agccaatcac gacttccgca ctttaatgcg cgtcacgcgt ctgaaagaag atgtgctgaa
    40801 agaagccgtc aatctgatcc agtcgctcga tccgcgcccc gggcagtcga tccagactgg
    40861 cgaacctgag tatgtcattc cagatgtgct ggtgcgtaag cataacggtc actggacagt
    40921 agaactcaac agtgacagca ttccgcgtct gcaaatcaac cagcactacg cctcgatgtg
    40981 caataacgct cgcaatgatg gtgacagcca gtttatccgc agcaatctgc aggatgccaa
    41041 atggttgatc aagagtctgg aaagccgtaa cgatacgcta ctgcgcgtga gtcgctgtat
    41101 cgttgaacag cagcaagcct tctttgagca aggcgaagaa tatatgaaac cgatggtact
    41161 ggccgatatc gcccaggctg tcgaaatgca tgaatcgacg atatctcgcg tgaccacgca
    41221 aaaatacctg catagtccac gaggcatttt tgaactgaag tatttctttt ccagtcacgt
    41281 caataccgag ggcggcggcg aagcttcctc cacggcgatt cgtgcgctgg tgaagaaatt
    41341 aatcgcggcg gaaaacccag cgaaaccgtt gagcgacagc aagttaacct ctttgctgtc
    41401 ggaacaaggt atcatggtgg cacgccgcac tgttgcgaag taccgagagt ctttatccat
    41461 tccgccgtca aaccagcgta aacagctcgt ttgacccaac cgataaggaa gacactatgc
    41521 agctcaacat taccggaaat aacgtcgaga tcaccgaggc actgcgcgaa tttgttacag
    41581 ccaaatttgc caaacttgag caatattttg accgaatcaa ccaggtctat gttgttctga
    41641 aagtggagaa agtcacccac acctcagatg caacactgca tgtaaacggt ggtgaaattc
    41701 atgccagcgc ggaaggtcag gacatgtacg ctgccattga tggcttaatt gataagctgg
    41761 cacgccagtt gaccaaacat aaagataaac tgaaacaaca ctaattgtcc gggcaattag
    41821 catgtgcatg gcggcctgtt gtgcggcaca acgggccatt tgtacggtta atgctccgag
    41881 cctgttccac tgtttgagtg ggcaggttct taggtgaaat tatgacaaat aatgatacaa
    41941 ctctacagct tagcagtgtt cttaacaggg aatgtacgcg aagccgcgtc cactgtcaga
    42001 gcaaaaaacg cgccctggaa atcatcagcg agctggcggc gaaacaactt agcctgccgc
    42061 ctcaggtggt ttttgaagct atcctgacgc gggaaaaaat gggcagtacc ggtatcggca
    42121 atggtattgc cattccgcat ggcaaactgg aagaagatac tctgcgcgcc gttggcgttt
    42181 tcgttcagct cgaaacgcct atagctttcg atgccatcga caaccaaccg gtggacttac
    42241 tttttgccct gctggtgccg gcagaccaaa ctaaaacgca cctgcatact ctgtcgctgg
    42301 tggcgaaacg tctggcggac aaaaccatct gccgccgttt acgcgcagcc cagagcgatg
    42361 aagagctgta tcaaatcatt acggataccg aaggtactcc ggatgaagcg tagttattcg
    42421 gtaatgtctc ttttagacgt tgtgaggaga aacagtacat ggtactgatg atcgtcagcg
    42481 gacgttcagg ttcaggtaaa tctgtcgccc tgcgtgcgct ggaagatatg ggtttttact
    42541 gcgtggataa ccttcccgta gtgttgttac ccgatctggc tcgaactctg gccgatcgcg
    42601 agatttctgc cgccgtcagc attgatgttc gtaatatgcc ggagtcacca gaaatattcg
    42661 aacaggcgat gagtaacctg cctgacgctt tctcaccgca actactgttc ctggatgccg
    42721 accgtaatac cttaattcgt cgttacagtg acacgcgccg actgcatccg ctttccagca
    42781 aaaacctgtc gctggaaagt gctatcgaca aagaaagcga tttgctggag cctctgcgtt
    42841 cgcgagcgga tctgattgtc gatacctcag aaatgtccgt tcacgagctg gctgaaatgc
    42901 tgcgtacccg tctgctgggt aaacgtgaac gcgaactgac catggtcttt gagtctttcg
    42961 gcttcaaaca cggtatccct atcgatgcag attacgtctt tgacgtgcgc ttcttgccga
    43021 acccgcactg ggatccgaaa ctgcgtccaa tgacaggtct tgataaacct gtcgccgcgt
    43081 tcctcgaccg ccacacagaa gtacacaatt ttatctacca gacgcgaagc tatcttgaac
    43141 tatggttacc tatgctggaa accaacaacc gtagctactt gacggtcgcc attggttgta
    43201 ccggcggaaa gcaccgttcg gtgtatattg cagagcaact ggcagactac ttccgctcgc
    43261 gcggtaaaaa cgtccagtca cgccatcgta cgctggaaaa acgtaaacca tgaccgtcaa
    43321 gcaaactgtt gaaatcacaa acaagctggg catgcatgcc cggcctgcaa tgaagctgtt
    43381 tgaattaatg cagggttttg acgctgaagt gctgttacgt aatgacgaag gcaccgaggc
    43441 cgaagccaac agcgttattg cgctgctgat gttggattct gccaaaggac ggcagattga
    43501 agttgaagcg accggcccac aggaagagga agcactggcc gccgttatcg ccctctttaa
    43561 ctctggtttt gatgaagatt aatcttcatc actttgacat acaactccct tcaaactccc
    43621 cccgacaata agaaaatcac gtactgaaat cgttctcaat caacgtcatt tgtacatttt
    43681 gtgcgctttt cactttcaga agaaccttaa gaaaacctta agagacattg tttaggtttt
    43741 gtttaagtta atcgaccata ctggagatcg tcagaaaata tttccaggag atggcatgat
    43801 tcgcttatct gaacaaagtc ccctgggcac cggaagacat cggaaatgtt atgcgcatcc
    43861 ggaagatgcc caacgctgta tcaagattgt ctaccatcgt ggcgatggtg gcgataaaga
    43921 gatccgccgt gagttaaagt actacgcgca tcttggtcgc cggttaaaag actggagtgg
    43981 aataccgcgc tatcacggta ccgttgagac ggattgcgga accggttatg tctacgatgt
    44041 gattgcagat tttgacggca aaccctccat tacgctgacc gaatttgctg aacaatgccg
    44101 ttacgaagaa gacattgcac aactgcgcca gctactgaaa cagctgaagc gttatttgca
    44161 ggataaccgt atcgtgacga tgtcgctgaa gccgcagaat atcctctgtc accgcatcag
    44221 tgagtcagag gttatcccgg tcgtctgtga caacattggt gaaagcacat tgattccact
    44281 ggcgacctgg tcgaagtggt gttgtctgcg taagcaagag cggttatgga aacgatttat
    44341 cgcccaaccg gcgctggcta ttgccctgca gaaagatctg caacctcgtg agagcaaaac
    44401 gctggccctc acttcgcgcg aagcttaatc cagctggtgt tgttgcataa acggctcacc
    44461 gcctaactga tacatctgcc gtaaaatcca cgcctgacgg ctacgcacgt agcccgatgg
    44521 cgaggagact ttgaaacgaa gtggattagg taatacagct gccagtaatg cagcttccga
    44581 ccgggtaagt ttgctcgcgg gtttgtggaa ataacgttgt gccgcagctt cgacgccaaa
    44641 cacaccgtcg ccaaattcgg cgatattcag gtaaaccgtc aggatacgct ttttgctcca
    44701 gactgtttct atccccagcg ttaatccagc ttccagccct tttcgtaccc agctacgccc
    44761 atcccataaa aagagatttt tggccgtctg ctgagaaatc gttgaagcac cgcgaatacg
    44821 gttttcattg cgctcgttgt gcgccagggc tttctcaatg gaagcgacat caaagcccca
    44881 gtgctcagga aatttctgat cttctgcggc aatcaccgcc agtcccatcc acggcgagat
    44941 ttgatccata ctgacccagt cagaatgtgc cacgtaacga aaattgccat gcagccaggc
    45001 gctgacctgt cgctcgacca ttaccgctga gaagggaaca ggcgcaacac taaacaacgc
    45061 gatgcccccg ccccagaaaa cggcgaggac aaccattaac cgcaaaagaa aacggcgaac
    45121 gaaactaaac accgttaagc ggcttttact cattcagcca gaaccagcac gcgggaaacc
    45181 agcttatcaa tgccgctcgc cgcttctgca atgttctgcg ccagcatata tgctggggtg
    45241 gtgacaatct tattgtcttc atcaaccacg atatcatcga caggacaggg cacatgctcc
    45301 gcgcccatct cttccagcac ttctgcggta tcgatatcag taccgatggt caaacgcagc
    45361 gggaaatcga aaattttcgg cagcatcgcc ggggcaatac acataaaacc aagcggtttt
    45421 ccggcctgat gcatcgcttg tgccagcgcc tttaattcac ggtcaacggt gcattcgcta
    45481 ccaagagtgg caaaattgct taaattcttc gccgcgccaa acccccccgg cacaatcaac
    45541 gcatccagtt cagcggcatc ggcctgggcc agaggacgga tttcaccgcg cgttattcgt
    45601 gccgcttcaa tcagcacgtt gcgcgtttcc gtcatcgctt cgccagttaa atggttgata
    45661 acatcaacct gctgcttatc cggtgcaaag cagaccgcct gcgcaccgct gcgtgaaata
    45721 gctaacaacg tcaacaccgc ttcatgaatt tcagaaccgt catagacgcc gcatccgctc
    45781 agaattacgc caattttctt cattgtgatc atcctttcgc aattgactga aacacattaa
    45841 tttttttaat aaaaatggta cgcatcacac atttaactga ttcatgtaac aaatcattta
    45901 agttttgcta tcttaactgc gtgcggcctg aaaaacagtg ctgtgccctt gtaactcatc
    45961 ataataattt acggcgcagc caagatttcc ctggtgttgg cgcagtattc gcgcaccccg
    46021 gtctagccgg ggtcattttt tagtggcttt tgccacccac gctttcagca cttctacgtc
    46081 gtgacgccac tcttctttca tctcttcaat ccattcaccg acgttatctt cccaggcagg
    46141 aaggtccgga gactgaattt gctgacccag ctgttgcaga tggcgtaacc ccactgaccc
    46201 cgccgcacct ttaattttat gtccttcctc tacaatgcct tttttatcct gcgccgtcag
    46261 attcgactcc agcacgctga catagcccgg catcatcttc tcaaacaccg ctaacccgtc
    46321 ggtgatcagc ttcggtccta caagttcgag atactgttcc agcatgggaa tatcgagcaa
    46381 tgcttctgat ttactgttct cttctgtcgt caccgtactc tcctcatcat cctgggtatc
    46441 ccagaatttc ttgatcatcg cggttagcgc cggaaccgaa agcggcttac tcagcacatc
    46501 atccattcca gcattgaggt actcttgttt gtctttcagc acgttagcgg ttaaggccac
    46561 cagcggcggt aaatcctcgc gcggataacg cttcgtcagt tcacgagaga tatccagccc
    46621 ggtcatatct ggcaactgaa tatccagcaa caccaggtcg tattcgcccg gtttaaacat
    46681 ctccagcgcc gccttgccgg tcatggcgac atcaacgctg ttacctaatt tttccagcac
    46741 agaacgcgca acaatcacgt tcagttcaat gtcttccacc agcagcacat tcagcgccgg
    46801 taaaggcata tcgtcttcat caaacgcatc atcgacctct tctgctaccg acggtgcgtg
    46861 gatcgtcaac gtaaaggttg aacctttgcc ctgttcgctg gtaaccgtaa tatcgccgcc
    46921 catatttttc gccagacgac gagaaacggc cagaccaata ccggtgccgg tggcaggttt
    46981 accgccatga ctgtctttca cctggtaata catggcgaaa attttatcca gctcatcctg
    47041 cggaatgccg ataccagagt cttccacttc aaaatgcagc atatcgcctt catcgtagcg
    47101 cacgcgcacg gtaacctggc cttgctgggt gaatttgacg gcgttactga tgaggttcca
    47161 caggatctgc cgtaaacgcg tcccgtcggt aatgacctga tgcggtaatg gcagcgtcgg
    47221 ctccaggtta aagcgcaatc ctttttgttg cgcctgcaat gcggagagat tttccagatc
    47281 ggcaaggaag ctggtgaaat caaccggttg attatcaagc tggaccttgc gccgttccat
    47341 cttatccatg tcgataatat cgttaaagat attccccagc gtgacggccg aaacatggat
    47401 ggtcttgaga tatttttcct gctcggcggt gagttcggta tccagcagaa tacggctcag
    47461 gccgacgata ccgttcagcg gtgtacgcaa ttcgtgactg atggtggaga taaacgtcgt
    47521 tttgtcgcgg ctggcccgtt caagcgcatc ctgataccgc ttacgctcgg taatgtcgcg
    47581 accaaagccc atcaaaccgt gacgtttacc cacgcggtcg tagtacggca ctttacggat
    47641 ttcaaagcag gctttgcgcc cgtccgggta atccagccac tgttcatagg tcagtgacac
    47701 attatgacgg aacacttttt catcggtttc aatgactttt gcggcggctt ccggtgagta
    47761 aacatcagca ggtttcaggt gaaccagttg tttttcgctt tttccggtca gcagctccat
    47821 cgcgcggtta cagccggaaa actctttatc ttcgttacga taaaaaacca ggtcgggtga
    47881 agcatcaagg aaggaacgta agaatgagga ttgctgctcg agctgaattt gtgtctcttc
    47941 gcgctctttg atttcaattt tcagttggcc gaaggtttcc tgcagttctg cttccgcttt
    48001 ttcacgaacg gcgatttcct gatttagctg ggcaatatta tcttttaact gaacgttgag
    48061 gctcaaatcg cgctcgcgca tctcctccag tttttgcacc agccgtgaca gacgttgtcg
    48121 tgactcctcc agttgctcga cgaccaccga tagaaagtag accgcccacg gcgtaatcag
    48181 caaaccaaag aagatagaac gaataacatc aatgctttcg acctgaccat gcagcaccat
    48241 ggttaccgcc atttgtacca caatggcaag aacgacgagg gccagcgcca gcaacattga
    48301 gaagcgcacc agacctaact tcatcatcag gtcaacataa tactgcgcca gcagacgaat
    48361 ttgcttcatt agggaattcc ttcacgacaa cctgccccaa taatacccaa ttctgacagt
    48421 tacgttataa attgtgtgag aaatgcggaa ttcccctcaa cgacctactc cgttggaggt
    48481 atccacggac gttccagcgc cgatccctgt acgccatgtt cattcagata gcgatccagc
    48541 tcgaccattc ccgtccatcg attttcgcac cacagaggag caagcagcgt cgggcgacgg
    48601 gcgctggcgg aaatgcggtg gtagatcact tccggtggcg tatggcgaat catttctccg
    48661 gcagtgagcg tgtaatcctc cagttcaata ccgttcaaac gccccgcttc ccaggctttc
    48721 gccataatgc tgcctttcac aatatgcagc ggatgcagct ttatgccgtc cacgccggtt
    48781 tcaaccactc gctccagcgt ttgcaaacat tcagcctgcc cttcgccagg caggccgaca
    48841 attaagtggg aacacacctt cagcccgcgc tgacgtgcca gctgggttgt acgctgataa
    48901 caggcaaaat catggccgcg gttgatgcga tgcagtgttt tgtcgtgggc ggtttgtagc
    48961 cccagctcca gccacacttc gtaaccctgg tccttatatt cgcaaagcag atccagcacc
    49021 gcatccggca cgcagtccgg gcgggtacca acacacaaac cgacaatatt ggcctggctc
    49081 accgcctgct gatacataga acgcagcacc tgaacttccg caaaggtgct ggtatacgcc
    49141 tgaaagtagg ccagatagcg tttagcgcgg ttaactaaat tcgcctggtg cgccagttgc
    49201 tcggcaatgg aacgatgctg ctgcgcttca tcggcaaacg aggcaacatt acagaatgtg
    49261 cagccaccac gcccgatggt accgtcacgg ttagggcagc taaaaccgcc gtgcagcgtc
    49321 agcttatgca ccttttgccc ataacgacgg gtgagatcac caccaaacat attgactaat
    49381 ttctgtaact gcataatctg atagaccgcg ccttgaaaag aggccaaagc ctgccatttt
    49441 tagctcaatt cggcgatgac ctggatcaat cgtcccggcc tgcttttatc aactgcataa
    49501 tcaatcaaaa ttaccgaaat ttcatgcata atcacataaa tcacttttgc ttatcttgtg
    49561 tcagattttt ttatctcctt atggatttta ggcaaaaaca gtagcatgaa acgtcattac
    49621 caattaaggc agtataaaat gctggctttc tcgtcagttc aaggcaggat aagggttaac
    49681 acgcctttat gacagtcagg aattgactgt ttctctaacg acttcccttt tagccttaaa
    49741 gataaaatct attttaattt cagtcattta ataaagaatt ttgcgctaaa gcacatttct
    49801 gtaccaataa gcttgccatt tgacctgtat cagctttccc gataagttgg aaatccgctg
    49861 gaagctttct ggatgagcag cctgctcatc atatttatgc agtaattgag atcccctctt
    49921 caccgtatta accgatgcga aaaggacaac aagggggcga atgcgaggcg cgcgtatgac
    49981 acgcaaaccc cgtcgccacg ctctttctgt gcccgtgcgc agcggttcgg aagtggggtt
    50041 cccgcagagc ctgggggagg ttcacgatat gttgtacgat aaatcccttg agagggataa
    50101 ctgtggtttc ggcctgatcg cccacataga aggcgaacct agccacaagg tagtgcgtac
    50161 tgcaatacac gcactggccc gcatgcagca ccgtggcgcg attctcgccg atggtaaaac
    50221 cggcgacggt tgcggcttgc tgttacaaaa accggatcgc ttttttcgca tcgttgcgca
    50281 ggagcgcggc tggcgtttag caaaaaacta cgccgtcggg atgctattcc tgaataaaga
    50341 tcctgaactc gccgctgccg cacgccgcat cgttgaagaa gaactgcaac gcgaaacctt
    50401 gtcgattgtg ggctggcgtg atgtccccac taacgaaggc gtgctgggtg aaatcgccct
    50461 ctcctctctg ccacgcattg agcaaatttt tgtgaacgcc ccggcaggct ggcgtccgcg
    50521 cgatatggag cgccgtctgt ttatcgcccg ccgccgcatt gaaaagcgtc tcgaagccga
    50581 caaagacttc tacgtctgta gcctgtcgaa tctggtgaac atctataaag gtctgtgtat
    50641 gccggcggat ctgccgcgct tttatctgga tcttgcggac ctgcgtctgg aatcggccat
    50701 ctgcctgttc caccagcgct tctccactaa caccgtaccg cgctggccgc tggcgcagcc
    50761 gttccgctac ctggcgcata acggtgaaat caacaccatc accggtaacc gccaatgggc
    50821 gcgtgcgcgt acctataaat tccagacgcc gcttatccct gatctgcacg acgccgcacc
    50881 gttcgtcaac gaaaccggct ctgactccag ttcgatggat aacatgctgg aactgctgct
    50941 ggcaggcggg atggatatca tccgcgccat gcgtctatta gtaccacccg cctggcagaa
    51001 caacccggat atggacccgg aactgcgtgc cttctttgac tttaactcca tgcatatgga
    51061 gccgtgggat ggcccggcgg gcatcgtgat gtccgatggt cgcttcgctg cctgtaacct
    51121 cgaccgtaac ggtctgcgtc cggcacgcta cgtcattacc aaagacaagc tcatcacctg
    51181 cgcctctgaa gtcggtatct gggattatca gcctgacgaa gtggtcgaaa aaggccgcgt
    51241 cgggccaggc gaactgatgg tgatcgacac ccgcagtggg cgtattctgc actcggcaga
    51301 aaccgatgac gatctgaaaa gccgccatcc atataaagag tggatggaga aaaacgtccg
    51361 ccgactggta ccgtttgaag atctgcccga tgaagaagtg ggtagccgcg aactggacga
    51421 cgacacgctt gccagctacc agaaacagtt taactacagc gcggaagagc tggactccgt
    51481 aattcgcgta ctgggcgaaa acggtcagga agcggtcggt tcgatgggcg atgatacccc
    51541 attcgccgtg ctctccagtc agccgcgcat tatttacgac tacttccgcc agcagtttgc
    51601 ccaggtgact aacccgccaa tcgacccgct gcgtgaagcg catgttatgt cgctcgccac
    51661 cagtatcggt cgtgaaatga acgtcttttg cgaagcagag ggccaggcgc accgtttaag
    51721 ctttaaatcg ccgattctgc tctactccga tttcaaacag ctcacgacga tgaaagagga
    51781 gcactaccgc gcagatacgc tggatatcac ctttgacgtc actaaaacca cgctcgaagc
    51841 gacagtcaaa gagctgtgcg acaaagccga aaaaatggta cgtagcggca ccgtgctgct
    51901 ggtgctctcc gaccggaata tcgctaaaga tcgcctgccg gttccagccc cgatggcggt
    51961 tggcgcgatc cagacccgtc tggtcgatca aagcctgcgt tgcgatgcca acatcatcgt
    52021 cgaaaccgcc agcgcccgcg atccgcacca cttcgccgtg ttgctgggct tcggcgcgac
    52081 ggctatttat ccataccttg cctatgaaac gctgggccgc ctggtagaca cccatgcgat
    52141 tgccaaagat tatcgtaccg tgatgctcaa ctaccgtaac ggcatcaaca aaggcttgta
    52201 caaaatcatg tccaaaatgg gcatctccac catcgcctct taccgctgct cgaaactgtt
    52261 tgaagcggtc ggtctgcacg atgatgtagt gggcctgtgc ttccaggggg cggtcagccg
    52321 cattggtggg gcgagctttg aagacttcca gcaggatctg ctgaacctgt cgaaacgtgc
    52381 ctggctggcg cgtaagccca tcagccaggg cggcctgctg aaatacgtcc acggcggcga
    52441 ataccacgct tacaacccgg acgtggtgcg cacgctgcaa caggcggtac aaagcggcga
    52501 gtacagcgac tatcaggaat acgcgaagct ggttaatgag cgtccggcaa ccacgctgcg
    52561 cgatctgctg gcaattacgc cgggtgaaaa cgcggtcaac attgctgatg ttgaaccggc
    52621 aagcgaactg tttaaacgct ttgataccgc cgcgatgtct atcggcgcgt taagcccgga
    52681 agcccacgag gcgctggcgg aagcgatgaa tagcatcggc ggtaactcga actccggtga
    52741 aggcggcgaa gacccggcgc gctatggcac taacaaagtg tcgcgcatca agcaggtggc
    52801 ttctggtcgc ttcggcgtta ctccggcgta tctggtcaat gccgacgtca ttcagattaa
    52861 agtcgcccag ggcgcgaagc cgggcgaagg cggtcagttg ccgggcgaca aagtcactcc
    52921 ttacatcgcc aaactgcgct attcggtgcc cggagtgacg ctgatctccc cgccgccgca
    52981 ccacgatatc tactctatcg aggacttagc gcagctcatt ttcgacctca agcaggttaa
    53041 cccgaaagcg atgatctccg tgaagctggt ttccgaaccg ggcgtaggca ccatcgcgac
    53101 tggcgtggca aaagcttatg ccgatttaat caccatcgca ggctatgacg gtggcaccgg
    53161 cgcaagcccg ctgtcgtcag tgaaatacgc aggttgcccg tgggagctgg ggcttgttga
    53221 aacccagcag gcgctggttg ccaatggtct gcgtcataag atccgtttgc aggtcgatgg
    53281 cggcctgaaa accggtgttg atatcatcaa agcggcgatt ctcggcgcag aaagcttcgg
    53341 cttcggcact ggcccgatgg tggcgctcgg ctgtaaatat ctacgtattt gccatctgaa
    53401 caactgcgca acgggtgtag caactcagga tgacaaactg cgtaagaacc actatcacgg
    53461 cctgccgttc aaggttacca attactttga atttattgcc cgtgaaaccc gcgagctgat
    53521 ggcacagctg ggcgtaacgc gtctggtgga tctgattggt cgcaccgacc tgctgaaaga
    53581 actggacggt ttcaccgcca aacagcaaaa actggcgctg tcgaagctgc tggagaccgc
    53641 cgaaccgcat ccaggtaagg cactctactg caccgaaaac aacccaccgt ttgataacgg
    53701 cctgctgaac gcgcagttgc tgcaacaggc gaaaccgttt gtcgatgaac gccagagcaa
    53761 aaccttctgg ttcgatattc gcaacaccga ccgttctgtc ggcgcgtcgc tttcaggcta
    53821 tatcgcccag acgcacggcg atcaggggct ggcagccgat cctatcaaag cgtacttcaa
    53881 cggcaccgca ggccagagtt tcggtgtgtg gaacgcgggc ggcgtggaac tgtacctgac
    53941 cggggatgcc aacgactatg tcggtaaagg catggcgggc ggcttaatcg ccattcgtcc
    54001 tccggttggt tccgccttcc gcagccatga agcaagtatt atcggcaaca cctgcctgta
    54061 tggcgcgacc ggtggtcgtc tgtatgccgc aggccgcgcg ggtgaacgtt tcggcgtgcg
    54121 gaactccggt gctatcaccg tggtagaagg cattggcgac aacggctgtg aatatatgac
    54181 gggcggtatt gtctgtattc tgggtaaaac cggcgttaac ttcggtgcgg gcatgaccgg
    54241 cggcttcgct tacgttctcg atgaaagcgg tgatttccgc aaacgcgtta acccggaact
    54301 ggtcgaggtc ttaagcgttg acgatctggc gatccatgaa gagcatctgc gcggtcttat
    54361 caccgagcat gttcagcata ctggctctca gcgcggtgaa gagattctgg cgaactggtc
    54421 aaccttcgcc actaaatttg cgctggttaa accgaagtcc agtgatgtaa aagcactgct
    54481 gggtcatcgt agtcgtagcg cagcagagtt gcgcgtgcag gcgcagtaag gggtagcaac
    54541 aatgagtcag aatgtttatc aatttatcga cctgcagcgc gttgatccgc caaagaaacc
    54601 gctgaagatc cgcaaaattg agtttgttga aatttacgag ccgttttccg aaggccaggc
    54661 caaagcgcag gctgaccgct gcctgtcgtg cggcaaccca tactgcgagt ggaaatgtcc
    54721 ggtacacaac tacatcccga actggctgaa gctcgccaac gaggggcgta tttttgaagc
    54781 ggcggaactg tcgcaccaga ccaaccccct gccggaagtt tgcggacgag tctgcccgca
    54841 agaccgtctg tgcgaaggtt cctgcactct gaacgatgag tttggcgcgg tgaccatcgg
    54901 caacattgag cgttatatca atgataaagc gttcgaaatg ggctggcgtc cggatatgtc
    54961 cggcgtgaag caaaccggta aaaaagtggc gattatcggc gcaggcccgg caggtctggc
    55021 gtgtgcggat gtcctgacgc gtaacggcgt aaaagccgtt gtcttcgacc gtcatccaga
    55081 aattggcggg ctgctgacct tcggtattcc ggccttcaag ctggaaaaag aggtaatgac
    55141 gcgtcgccgt gaaatcttca ccggcatggg tattgaattc aaactcaata ccgaagtggg
    55201 ccgcgacgtg cagctggacg atctgctgag tgattacgat gccgtcttcc ttggcgtcgg
    55261 gacttatcag tcaatgcgcg gcgggctgga aaacgaagac gccgatggcg tgtacgcagc
    55321 gctgccgttc cttatcgcca acaccaaaca gttaatgggc tttggtgaaa cccgcgacga
    55381 accgttcgtc agcatggaag gcaaacgcgt ggtggtcctt ggcggtggcg acactgcgat
    55441 ggactgcgtg cgtacgtccg tgcgccaggg agcgaagcac gttacctgtg cctatcgtcg
    55501 tgatgaagag aacatgccgg gttcccgccg cgaagtgaaa aacgcgcggg aagaaggcgt
    55561 agagttcaaa ttcaacgtcc agccactggg gattgaagtg aacggtaacg gtaaagtcag
    55621 cggcgtaaaa atggtacgta ccgaaatggg cgaaccggat gccaaaggcc gtcgccgcgc
    55681 ggagatcgtt gcaggttccg aacatatcgt tccggcagat gcagtgatca tggcgtttgg
    55741 tttccgtcca cacaacatgg aatggctggc aaaacacagc gtcgagctgg attcacaagg
    55801 ccgcatcatc gccccggaag gcagcgacaa cgccttccag accagcaacc cgaaaatctt
    55861 tgctggcggc gatatcgtcc gtggttccga tctggtggta accgctattg ccgaaggtcg
    55921 taaggcggca gacggtatta tgaactggct ggaagtttaa gctctgtccc agcgccgcct
    55981 cctcagaaaa gagtcggcgc tgaaattctc ctttctttct ggaaagcgcc tcgcataatc
    56041 tgaagaaaac caatgtaata cgctcatctg gattgttacc ggtagatgcc cggcgtatag
    56101 ctgattccat tattcaacgc agcgccatat aaagggacag ataatggaat cgctctcaga
    56161 gagaacctca acaggctacc agcaaatcca cgacggtatt attcatctgg tcgatagcgc
    56221 ccggacggaa acggtacgta gcgttaacgc gctgatgacc gcgacatact gggaaattgg
    56281 ccgacgaatt gtcgaatttg aacaaggtgg cgaggccagg gctgcgtatg gtgcgcagct
    56341 aatcaagcga ctatcaaagg atttaagtct aaggtataag cgtgggttct ctgcaaaaaa
    56401 cttacgccaa atgaggcttt tttacctctt ttttcaacat gttgaaattc gccagacagt
    56461 gtctggcgaa ttaacacctg ccctggtcca cctacgtccg tttactttcc gttaaaaacg
    56521 ctgacgcccg cagcttttat gaaaaagaga cgctccgctg tggctggtct gttcgccagc
    56581 tagaacggca aattgcgacc caattttatg agcgaacact actgtcacat gacaaatcag
    56641 ccatgctgca acaacacgct cctaccgaga cgcatattct tccgcaacag gcgatacgcg
    56701 atccctttgt gctcgaattt ctggaattga aagatgaata ttcagaatct gatttcgagg
    56761 aggcgctgat caaccacctg atggatttca tgctggaact tggggatgat tttgcctttg
    56821 ttggtcggca gcgaaggtta cgcattgatg acaactggtt tcgtgtcgat ctgctgtttt
    56881 tccaccgccg tttacgctgc ctactaatcg tcgatctaaa agtgggcaaa ttcagctata
    56941 gcgatgccgg acagatgaat atgtatctca actacgccaa agagcactgg acgctaccgg
    57001 atgaaaatcc gcccatcggt ctggttctct gtgcagagaa aggagccgga gaagcacatt
    57061 atgctctggc aggtttgcct aacaccgttc tggcaagcga atataagatg caactacctg
    57121 atgagaaacg actcgcagat gaactcgttc gaacacaggc ggtgctagag gaaggctata
    57181 gacgccgtta atttcagcgg tttcgaattc tgcagacagt gtctgcagaa tctttaaaac
    57241 gaactaagtt ttaagccatt aaatcagcct taaccttcac cacaaccttt ttaatctctc
    57301 caggctcgcc gacaacgcat cctggtttat gcggttcacc tggcataaac acggcgaaca
    57361 ttcccggttt taagatgatg gcttgctcgt tatcaatggt gctgcaaagc tgataatcat
    57421 cctcatggtg gaactcttca cactggcgcg cagtgcctgc catgccaaac agaatccgtt
    57481 cctcaccgtt taataacagt tggatatcaa tgtattgctc gtgcaattcc gcttttttct
    57541 cgacgggcga ttgagtgtta aacgtcatga cattcataaa gatattgtcg ccctgtaatt
    57601 cgtaacgacc cggcgctttt tcttgcggtc tggcagctaa tgccagcgtt aacgcgtcct
    57661 gtaacgcagg atgtaaccca gcagacggta atgactgtac ttcacccatc atcataattt
    57721 ttctccctgg gccaacagcg cagccccaag taaacctgca tcgtggcggt agtgcgccgc
    57781 cagtaaatca acatgaaatg ccgctggctc ctgcgccaga tacgtttcca ccagcgccag
    57841 atacccttct gccagcccaa cgctgccacc gaccaccacg cactggcaat cagttgtggc
    57901 tttaatatca gcgatcagcc ttgcaagcgt acgtgcggag cggtgaatca gctgctgcgc
    57961 ctgctcgtcg ccctgcccgg cgcgtgtgaa aatagttctc gcatccgcgc ccgccagttc
    58021 accctgcgct gccgccgcaa tgccgcgacc agaagcaatc gcctccacgc aacccgtgcg
    58081 tccacagcca cagaccgggc cgtgtggatc ggcaagcgta tgcccgatgt gccccgccag
    58141 accgccaggg ccggtaagca gtttgccgcc gctcactaca ccgccgccga cgccagtgga
    58201 aacggtgata aagaccatat cggttatatc gccttccagc gcctgatact ctgcccatgc
    58261 tgcggcctgc gcgtcgttaa tggcaatggt cggcaaattg gtaagttgtt ccagcgtttt
    58321 gactaacgga aagtgtagca atccaccgag attatgcgga ttcagcgcca gcaaactgcc
    58381 gtcgcgaata attccggttg aagcgatggc aacccgctgc gcatgagctt gcaacggaga
    58441 gactaatgcg gataaggcat cacgcaaggc ttctggtgtc tggctggctg gcgtaggaag
    58501 ttcacgacga tcgcggatct gcccgtcagc gccaatcagc gcggcggcaa gtttagtacc
    58561 gccgatatca atcgccagcg tggtcatagc accgcctttt tcatcgctgt gttgtaccac
    58621 tgacaaatgt gttcaagacg cgtaatggca gaaccgaccg tcaccgccca cgcgccgtgg
    58681 cgcatcgcat ctgccgcctg agcaggcgtg ttgtaacgcc cttcggcaat caccctacat
    58741 ccggcgtcgc tcaacgtttt caccagcgcc agatccggct cttctggcgt ttcaggcgtg
    58801 gtatagccag aaagcgtagt gccaataatt tcggctccca gcttttggca tgccaggccg
    58861 tcttccggcg ttgagcagtc ggtcatcgcc agtaaaccgt gatggtgaat acgtgccagc
    58921 agcgtttcaa caggcaccgg acgcgggcgg tcggtgccgt caatggcgat aatgtccgcg
    58981 cccgcctgcg ccagcgcatc aacatcttca atataggccg tgatgcgtac cggagaatcc
    59041 tccagatcgc gtttcacaat tccaataatc ggcacgctca ccaccgcacg cgtggcttgc
    59101 agatttgcca caccttcaat gcgaatggca accgcgcccg cctgttctgc cgctaatgcc
    59161 atggcggcga cgatttcggg tttatcgagc gggctgtccg gaaccggctg gcaggagaca
    59221 atcaggccac cgttagcagc gattttttga tccagttgtg caagtaacga catacatctt
    59281 cccttagcga aaggcccggt acatagaccg ggcaacagga ttaacttttg gttttgacta
    59341 aatcgttttt ggcgctgcca aatggcacgg caccgctaaa tggtttgccg tcgatagcgt
    59401 catgagtacg caatgcttcc gggcgcagcc aacgctgaac gcgggaaggc atatccagac
    59461 caatcagcag gatcaccaca aatgtcagac tgaacgatag cgatgccagc gcagtaccca
    59521 gatccagacg ttgagcgatc aacgcgccga tgattggggc cagtgcgccg cccaatgcac
    59581 caacgttgta ggtaaagccc aggcccgctg cacgctggtc ggtatcgaaa taaccgccaa
    59641 tcagtttcgg taagatcccg gcgatccctt gtccaagcat ttgctggaag aacagtaaca
    59701 gaccgagcac ccagacgttt gcgccgccaa tcgcaaatac cggaataatc aacagctgcg
    59761 aggccaacag gctacaaacg tacgctttgc gggttcccag ccagtcaccg aggaagccgc
    59821 cgacacagca ccccaccgca gccccaaagc cgctaaagaa cagcacattg gcgacagtat
    59881 gcgggttata agccagatcg gttttcagat acgttggcag cagcgcctga atcggccatg
    59941 agtagaggaa agcaaacaac acgaccacca tcagcattac gcccgttggc cagcgtttac
    60001 ccgtactttg caccataaag ctgataaaga ttgcggcgca taacagccca agaacagcga
    60061 cgatcgcggc attttgcagg ttaccggcga agcaaaacca cagcgcagta gccgccgcca
    60121 gtgtcattac gatattggca atgcgatgtt caccacggta gagaatatct accatcgtgc
    60181 gtactggtgc tttacctgca tgtttctctt tccagtcttc cgcttccggg atgtttttac
    60241 gcagccagag agcaaagatg ataggcaaaa tgccgataaa gaacagcgca cgccagcccc
    60301 agaccggaac caccaggcta tagacctgag cggcaacgac ggcccccaca gagaagcctg
    60361 aaatcagaaa accactggct ttgttacgca gatgttttgg ccagctttca atgacatagg
    60421 tggcgctgga accgtattca cccgccatcc ccatgccgat gaccagacgc gcgataaaca
    60481 tggtgatgta gcctggcgca aagccgcagg ccagcgtccc ggccgagaag agaacgatgc
    60541 tggtgaccat tgccagacga cgcccgtagc ggtcacccat agcgccgagc atcaggccgc
    60601 cgaaccagcg agagataaag gctgcggaga tcagacttgc cgcctgcacc gtcgtcagcc
    60661 cgaattcacc ttgtacttcg gtgagtacca gggcgattaa aacgaaatca aaaccgtcaa
    60721 gcagatatcc caaccaggca gcggaaaatg cccgccattg tgcacgattg agatggcgat
    60781 accacgggat attctgggtt gtagtactca ttgtgagtct cccgcggtgg gcgatgccca
    60841 cacgctttgg tatgaaaatt gtagggtaca gatgcgttta tttcccctca cccggtaggg
    60901 gcgagcgagg ggaaaacaac tcacccgcgc tcttgcatca actgctgggc cagcgccttc
    60961 agttctggca gatatttttc atctaccggt ccaaacggtt tgcggcacag cggcacagaa
    61021 acgacatcca tataatggag gacagttttc aggccgcgga atacgcccgt tttgatcagt
    61081 aaatcaatga ctttattgca ttcagtttgc agtttctgcg cggtctggat atcgccttct
    61141 ttcagcgcct taacgatccc ctgatagcgc cagcccatga tgttgtaggt actaccgata
    61201 ccaccatcag cgcccgccag cagaccagag gcgaagattt cgtcgtaacc gttatagagc
    61261 acaagatcag gatgttcacg acggatctgc tccatctgat agagatcgcc agaagtctgt
    61321 ttcagcgcac cgacgccagg caacgtaacc agggtgttga tctgatccag ggtcagtttt
    61381 accccactca gggctggaat gttgtacacc accatcggca aaccatccgc tgaatcaata
    61441 attgcccgat agtgatcgca gtgttcttca aagctgaaag gatagtagaa cggcgtgacg
    61501 gcggagacgg catcgaagcc ataacgttta gccgatgccg caagttgttg gctttcggcg
    61561 gtgctgacgc aaccgacgtg ggcgatgagt ttaatcttac ctttcgcctc ttcggcgacg
    61621 atttccagta cctgttcacg ctcggaaagg ctttgtacaa aggcctcgcc ggtcgaacca
    61681 cccacgtata aaccgtcgat gccctgctga atattgaact gaaccaggcg acgcagactc
    61741 gctttatcca gtgcttgttg ttgatcaaaa ggagtcagga gtgcagccat tacgccacgt
    61801 aaattcgttg ccataaatac ctctgaagtg atgcttgtct gataaacgat atacctttat
    61861 acctgttata ccagatcaat taagcaacac cccatacaga aagcttataa tgcgatctgc
    61921 ttcactaaag tggcattatt tctttttgtt ggtggtctga ccgaaagcgt gccaggtagc
    61981 agagacgctg ttgagatgcg attgcaacgc acgatcggct tcgtcaggat catgacggcg
    62041 gatcgcatca acgatcgcaa tatgctgttg ataactaacg ttgttatgtt cgtgcagtgc
    62101 ctgatcggta accgttgggc gtgcggcaat aagccagtcg agcagggcaa cgtggatcgc
    62161 catgaagatt gggttaccgg ggatctccgc cagcacgcgg tggaaatcaa cgtctgaacg
    62221 aatgaatgcc gcgttgttat ccagcgactg actgttaatt tccagtgctt ttgccagcaa
    62281 atcgatttgc tcatcggtgg catgttcagc cgcatagcgc accaggctgg attcaaagaa
    62341 cagacgtaat tgttcgaaat gggcaatccc accaggatga gaaaggaaat ctttcgccat
    62401 gccggaaagc tcgccgatga tggtgtccgc agaaggacgt gagacgcggg cacgttcgcc
    62461 gttgttgatt tgcaccagac ctttgcgttt taacgctgcc agcgcttcgc gcacagaagg
    62521 acgcccgacg ttaaagaacg ccatcagttc gcgttcagac ggtaattgtt caccttcgcc
    62581 aaattcacga cggcggatca tctgttccag ctcttcttcc accatttcgg agagtttttt
    62641 acgcgccagc gggcggctac gcaagttgcg accaattgca ggtgaagaat cttcggtttg
    62701 cgaatcaaat gcgttcataa ggcccattct gtaaggtcag tgtgattaac atcatcagtg
    62761 acatcctatc acaggattga aagtagggga aaatggcagg tttttctctt tgtgcctcat
    62821 cattaccata attaacggaa taattaacta ttgcgaaaaa ttaatgtaac gcagataaa
//
LOCUS       DAZFWL020000014        60716 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFWL020000014 DAZFWL020000000
VERSION     DAZFWL020000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 60716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.014"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..802
                     /gene="ynaI"
                     /locus_tag="ACHKNP_RS04810"
                     /old_locus_tag="pgaptmp_000962"
     CDS             <1..802
                     /gene="ynaI"
                     /locus_tag="ACHKNP_RS04810"
                     /old_locus_tag="pgaptmp_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HGU4703174.1"
                     /translation="IDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDITSARIMSRI
                     IKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPF
                     SIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRI
                     TTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTK
                     TTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
     gene            complement(853..2466)
                     /gene="mppA"
                     /locus_tag="ACHKNP_RS04815"
                     /old_locus_tag="pgaptmp_000963"
     CDS             complement(853..2466)
                     /gene="mppA"
                     /locus_tag="ACHKNP_RS04815"
                     /old_locus_tag="pgaptmp_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HGU4703175.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASMENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2803..3702)
                     /gene="pgrR"
                     /locus_tag="ACHKNP_RS04820"
                     /old_locus_tag="pgaptmp_000964"
     CDS             complement(2803..3702)
                     /gene="pgrR"
                     /locus_tag="ACHKNP_RS04820"
                     /old_locus_tag="pgaptmp_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HGU4703176.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGHHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            3840..4760
                     /locus_tag="ACHKNP_RS04825"
                     /old_locus_tag="pgaptmp_000965"
     CDS             3840..4760
                     /locus_tag="ACHKNP_RS04825"
                     /old_locus_tag="pgaptmp_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4703177.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVSYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSIFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            4760..5692
                     /locus_tag="ACHKNP_RS04830"
                     /old_locus_tag="pgaptmp_000966"
     CDS             4760..5692
                     /locus_tag="ACHKNP_RS04830"
                     /old_locus_tag="pgaptmp_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HGU4703178.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQKALEKVIPFFNTHLG"
     gene            5710..6348
                     /locus_tag="ACHKNP_RS04835"
                     /old_locus_tag="pgaptmp_000967"
     CDS             5710..6348
                     /locus_tag="ACHKNP_RS04835"
                     /old_locus_tag="pgaptmp_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4703179.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVSALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            6657..7385
                     /gene="mpaA"
                     /locus_tag="ACHKNP_RS04840"
                     /old_locus_tag="pgaptmp_000968"
     CDS             6657..7385
                     /gene="mpaA"
                     /locus_tag="ACHKNP_RS04840"
                     /old_locus_tag="pgaptmp_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HGU4703180.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPATAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(7360..8325)
                     /gene="ycjG"
                     /locus_tag="ACHKNP_RS04845"
                     /old_locus_tag="pgaptmp_000969"
     CDS             complement(7360..8325)
                     /gene="ycjG"
                     /locus_tag="ACHKNP_RS04845"
                     /old_locus_tag="pgaptmp_000969"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HGU4703181.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFVDLDGPTWLAVDVEPALQFTTGELHL"
     gene            8444..8950
                     /gene="tpx"
                     /locus_tag="ACHKNP_RS04850"
                     /old_locus_tag="pgaptmp_000970"
     CDS             8444..8950
                     /gene="tpx"
                     /locus_tag="ACHKNP_RS04850"
                     /old_locus_tag="pgaptmp_000970"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGU4703182.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(8994..10535)
                     /gene="tyrR"
                     /locus_tag="ACHKNP_RS04855"
                     /old_locus_tag="pgaptmp_000971"
     CDS             complement(8994..10535)
                     /gene="tyrR"
                     /locus_tag="ACHKNP_RS04855"
                     /old_locus_tag="pgaptmp_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HGU4703183.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(10683..11744)
                     /gene="ycjF"
                     /locus_tag="ACHKNP_RS04860"
                     /old_locus_tag="pgaptmp_000972"
     CDS             complement(10683..11744)
                     /gene="ycjF"
                     /locus_tag="ACHKNP_RS04860"
                     /old_locus_tag="pgaptmp_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HGU4703184.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(11741..13138)
                     /gene="ycjX"
                     /locus_tag="ACHKNP_RS04865"
                     /old_locus_tag="pgaptmp_000973"
     CDS             complement(11741..13138)
                     /gene="ycjX"
                     /locus_tag="ACHKNP_RS04865"
                     /old_locus_tag="pgaptmp_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HGU4703185.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            13293..14291
                     /gene="ycjW"
                     /locus_tag="ACHKNP_RS04870"
                     /old_locus_tag="pgaptmp_000974"
     CDS             13293..14291
                     /gene="ycjW"
                     /locus_tag="ACHKNP_RS04870"
                     /old_locus_tag="pgaptmp_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4703186.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKAEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(14402..15307)
                     /gene="ompG"
                     /locus_tag="ACHKNP_RS04875"
                     /old_locus_tag="pgaptmp_000975"
     CDS             complement(14402..15307)
                     /gene="ompG"
                     /locus_tag="ACHKNP_RS04875"
                     /old_locus_tag="pgaptmp_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HGU4703187.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(15352..16434)
                     /locus_tag="ACHKNP_RS04880"
                     /old_locus_tag="pgaptmp_000976"
     CDS             complement(15352..16434)
                     /locus_tag="ACHKNP_RS04880"
                     /old_locus_tag="pgaptmp_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4703188.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(16448..17107)
                     /gene="pgmB"
                     /locus_tag="ACHKNP_RS04885"
                     /old_locus_tag="pgaptmp_000977"
     CDS             complement(16448..17107)
                     /gene="pgmB"
                     /locus_tag="ACHKNP_RS04885"
                     /old_locus_tag="pgaptmp_000977"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGU4703189.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(17104..19371)
                     /gene="ycjT"
                     /locus_tag="ACHKNP_RS04890"
                     /old_locus_tag="pgaptmp_000978"
     CDS             complement(17104..19371)
                     /gene="ycjT"
                     /locus_tag="ACHKNP_RS04890"
                     /old_locus_tag="pgaptmp_000978"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HGU4703190.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(19368..20423)
                     /gene="ycjS"
                     /locus_tag="ACHKNP_RS04895"
                     /old_locus_tag="pgaptmp_000979"
     CDS             complement(19368..20423)
                     /gene="ycjS"
                     /locus_tag="ACHKNP_RS04895"
                     /old_locus_tag="pgaptmp_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4703191.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(20433..21221)
                     /gene="ycjR"
                     /locus_tag="ACHKNP_RS04900"
                     /old_locus_tag="pgaptmp_000980"
     CDS             complement(20433..21221)
                     /gene="ycjR"
                     /locus_tag="ACHKNP_RS04900"
                     /old_locus_tag="pgaptmp_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGU4703192.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEEPAQAYRDSLAWLRTC"
     gene            complement(21239..22291)
                     /gene="ycjQ"
                     /locus_tag="ACHKNP_RS04905"
                     /old_locus_tag="pgaptmp_000981"
     CDS             complement(21239..22291)
                     /gene="ycjQ"
                     /locus_tag="ACHKNP_RS04905"
                     /old_locus_tag="pgaptmp_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HGU4703193.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNLIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(22322..23164)
                     /gene="ycjP"
                     /locus_tag="ACHKNP_RS04910"
                     /old_locus_tag="pgaptmp_000982"
     CDS             complement(22322..23164)
                     /gene="ycjP"
                     /locus_tag="ACHKNP_RS04910"
                     /old_locus_tag="pgaptmp_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU4703194.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(23151..24032)
                     /gene="ycjO"
                     /locus_tag="ACHKNP_RS04915"
                     /old_locus_tag="pgaptmp_000983"
     CDS             complement(23151..24032)
                     /gene="ycjO"
                     /locus_tag="ACHKNP_RS04915"
                     /old_locus_tag="pgaptmp_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU4703195.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(24053..25345)
                     /gene="ycjN"
                     /locus_tag="ACHKNP_RS04920"
                     /old_locus_tag="pgaptmp_000984"
     CDS             complement(24053..25345)
                     /gene="ycjN"
                     /locus_tag="ACHKNP_RS04920"
                     /old_locus_tag="pgaptmp_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4703196.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(25359..27038)
                     /gene="ycjM"
                     /locus_tag="ACHKNP_RS04925"
                     /old_locus_tag="pgaptmp_000985"
                     /pseudo
     CDS             complement(25359..27038)
                     /gene="ycjM"
                     /locus_tag="ACHKNP_RS04925"
                     /old_locus_tag="pgaptmp_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(27250..27564)
                     /gene="pspE"
                     /locus_tag="ACHKNP_RS04930"
                     /old_locus_tag="pgaptmp_000986"
     CDS             complement(27250..27564)
                     /gene="pspE"
                     /locus_tag="ACHKNP_RS04930"
                     /old_locus_tag="pgaptmp_000986"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HGU4703197.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(27639..27860)
                     /gene="pspD"
                     /locus_tag="ACHKNP_RS04935"
                     /old_locus_tag="pgaptmp_000987"
     CDS             complement(27639..27860)
                     /gene="pspD"
                     /locus_tag="ACHKNP_RS04935"
                     /old_locus_tag="pgaptmp_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HGU4703198.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(27869..28228)
                     /gene="pspC"
                     /locus_tag="ACHKNP_RS04940"
                     /old_locus_tag="pgaptmp_000988"
     CDS             complement(27869..28228)
                     /gene="pspC"
                     /locus_tag="ACHKNP_RS04940"
                     /old_locus_tag="pgaptmp_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HGU4703199.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(28228..28452)
                     /gene="pspB"
                     /locus_tag="ACHKNP_RS04945"
                     /old_locus_tag="pgaptmp_000989"
     CDS             complement(28228..28452)
                     /gene="pspB"
                     /locus_tag="ACHKNP_RS04945"
                     /old_locus_tag="pgaptmp_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HGU4703200.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(28506..29174)
                     /gene="pspA"
                     /locus_tag="ACHKNP_RS04950"
                     /old_locus_tag="pgaptmp_000990"
     CDS             complement(28506..29174)
                     /gene="pspA"
                     /locus_tag="ACHKNP_RS04950"
                     /old_locus_tag="pgaptmp_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HGU4703201.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            29341..30318
                     /gene="pspF"
                     /locus_tag="ACHKNP_RS04955"
                     /old_locus_tag="pgaptmp_000991"
     CDS             29341..30318
                     /gene="pspF"
                     /locus_tag="ACHKNP_RS04955"
                     /old_locus_tag="pgaptmp_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HGU4703202.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(30438..31703)
                     /gene="puuE"
                     /locus_tag="ACHKNP_RS04960"
                     /old_locus_tag="pgaptmp_000992"
     CDS             complement(30438..31703)
                     /gene="puuE"
                     /locus_tag="ACHKNP_RS04960"
                     /old_locus_tag="pgaptmp_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HGU4703203.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(31741..33021)
                     /gene="puuB"
                     /locus_tag="ACHKNP_RS04965"
                     /old_locus_tag="pgaptmp_000993"
     CDS             complement(31741..33021)
                     /gene="puuB"
                     /locus_tag="ACHKNP_RS04965"
                     /old_locus_tag="pgaptmp_000993"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HGU4703204.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(33023..34510)
                     /gene="puuC"
                     /locus_tag="ACHKNP_RS04970"
                     /old_locus_tag="pgaptmp_000994"
     CDS             complement(33023..34510)
                     /gene="puuC"
                     /locus_tag="ACHKNP_RS04970"
                     /old_locus_tag="pgaptmp_000994"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HGU4703205.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLTDLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(34785..35342)
                     /gene="puuR"
                     /locus_tag="ACHKNP_RS04975"
                     /old_locus_tag="pgaptmp_000995"
     CDS             complement(34785..35342)
                     /gene="puuR"
                     /locus_tag="ACHKNP_RS04975"
                     /old_locus_tag="pgaptmp_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HGU4703206.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(35369..36133)
                     /gene="puuD"
                     /locus_tag="ACHKNP_RS04980"
                     /old_locus_tag="pgaptmp_000996"
     CDS             complement(35369..36133)
                     /gene="puuD"
                     /locus_tag="ACHKNP_RS04980"
                     /old_locus_tag="pgaptmp_000996"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HGU4703207.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            36345..37763
                     /gene="puuA"
                     /locus_tag="ACHKNP_RS04985"
                     /old_locus_tag="pgaptmp_000997"
     CDS             36345..37763
                     /gene="puuA"
                     /locus_tag="ACHKNP_RS04985"
                     /old_locus_tag="pgaptmp_000997"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HGU4703208.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            37912..38076
                     /locus_tag="ACHKNP_RS04990"
                     /old_locus_tag="pgaptmp_000998"
     CDS             37912..38076
                     /locus_tag="ACHKNP_RS04990"
                     /old_locus_tag="pgaptmp_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703209.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            38066..39451
                     /gene="puuP"
                     /locus_tag="ACHKNP_RS04995"
                     /old_locus_tag="pgaptmp_000999"
     CDS             38066..39451
                     /gene="puuP"
                     /locus_tag="ACHKNP_RS04995"
                     /old_locus_tag="pgaptmp_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HGU4703210.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALFVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            39585..39830
                     /gene="ymjA"
                     /locus_tag="ACHKNP_RS05000"
                     /old_locus_tag="pgaptmp_001000"
     CDS             39585..39830
                     /gene="ymjA"
                     /locus_tag="ACHKNP_RS05000"
                     /old_locus_tag="pgaptmp_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HGU4703211.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            40143..41786
                     /gene="sapA"
                     /locus_tag="ACHKNP_RS05005"
                     /old_locus_tag="pgaptmp_001001"
     CDS             40143..41786
                     /gene="sapA"
                     /locus_tag="ACHKNP_RS05005"
                     /old_locus_tag="pgaptmp_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HGU4703212.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            41783..42748
                     /gene="sapB"
                     /locus_tag="ACHKNP_RS05010"
                     /old_locus_tag="pgaptmp_001002"
     CDS             41783..42748
                     /gene="sapB"
                     /locus_tag="ACHKNP_RS05010"
                     /old_locus_tag="pgaptmp_001002"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HGU4703213.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            42735..43625
                     /gene="sapC"
                     /locus_tag="ACHKNP_RS05015"
                     /old_locus_tag="pgaptmp_001003"
     CDS             42735..43625
                     /gene="sapC"
                     /locus_tag="ACHKNP_RS05015"
                     /old_locus_tag="pgaptmp_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HGU4703214.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            43625..44617
                     /gene="sapD"
                     /locus_tag="ACHKNP_RS05020"
                     /old_locus_tag="pgaptmp_001004"
     CDS             43625..44617
                     /gene="sapD"
                     /locus_tag="ACHKNP_RS05020"
                     /old_locus_tag="pgaptmp_001004"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HGU4703215.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            44619..45425
                     /gene="sapF"
                     /locus_tag="ACHKNP_RS05025"
                     /old_locus_tag="pgaptmp_001005"
     CDS             44619..45425
                     /gene="sapF"
                     /locus_tag="ACHKNP_RS05025"
                     /old_locus_tag="pgaptmp_001005"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HGU4703216.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            45493..45846
                     /gene="ycjD"
                     /locus_tag="ACHKNP_RS05030"
                     /old_locus_tag="pgaptmp_001006"
     CDS             45493..45846
                     /gene="ycjD"
                     /locus_tag="ACHKNP_RS05030"
                     /old_locus_tag="pgaptmp_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HGU4703217.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            46214..47002
                     /gene="fabI"
                     /locus_tag="ACHKNP_RS05035"
                     /old_locus_tag="pgaptmp_001007"
     CDS             46214..47002
                     /gene="fabI"
                     /locus_tag="ACHKNP_RS05035"
                     /old_locus_tag="pgaptmp_001007"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HGU4703218.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            47147..48274
                     /gene="yciW"
                     /locus_tag="ACHKNP_RS05040"
                     /old_locus_tag="pgaptmp_001008"
     CDS             47147..48274
                     /gene="yciW"
                     /locus_tag="ACHKNP_RS05040"
                     /old_locus_tag="pgaptmp_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HGU4703219.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            48342..50276
                     /gene="rnb"
                     /locus_tag="ACHKNP_RS05045"
                     /old_locus_tag="pgaptmp_001009"
     CDS             48342..50276
                     /gene="rnb"
                     /locus_tag="ACHKNP_RS05045"
                     /old_locus_tag="pgaptmp_001009"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HGU4703220.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATAMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(50354..50458)
                     /locus_tag="ACHKNP_RS05050"
                     /old_locus_tag="pgaptmp_001010"
     CDS             complement(50354..50458)
                     /locus_tag="ACHKNP_RS05050"
                     /old_locus_tag="pgaptmp_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703221.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            50512..52497
                     /gene="pdeR"
                     /locus_tag="ACHKNP_RS05055"
                     /old_locus_tag="pgaptmp_001011"
     CDS             50512..52497
                     /gene="pdeR"
                     /locus_tag="ACHKNP_RS05055"
                     /old_locus_tag="pgaptmp_001011"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGU4703222.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWREKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(52479..52607)
                     /gene="ymiD"
                     /locus_tag="ACHKNP_RS05060"
                     /old_locus_tag="pgaptmp_001012"
     CDS             complement(52479..52607)
                     /gene="ymiD"
                     /locus_tag="ACHKNP_RS05060"
                     /old_locus_tag="pgaptmp_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HGU4703223.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            52645..52818
                     /gene="yciZ"
                     /locus_tag="ACHKNP_RS05065"
                     /old_locus_tag="pgaptmp_001013"
     CDS             52645..52818
                     /gene="yciZ"
                     /locus_tag="ACHKNP_RS05065"
                     /old_locus_tag="pgaptmp_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HGU4703224.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            52908..53657
                     /gene="yciT"
                     /locus_tag="ACHKNP_RS05070"
                     /old_locus_tag="pgaptmp_001014"
     CDS             52908..53657
                     /gene="yciT"
                     /locus_tag="ACHKNP_RS05070"
                     /old_locus_tag="pgaptmp_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HGU4703225.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            53926..54144
                     /gene="osmB"
                     /locus_tag="ACHKNP_RS05075"
                     /old_locus_tag="pgaptmp_001015"
     CDS             53926..54144
                     /gene="osmB"
                     /locus_tag="ACHKNP_RS05075"
                     /old_locus_tag="pgaptmp_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HGU4703226.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(54270..54596)
                     /gene="yciH"
                     /locus_tag="ACHKNP_RS05080"
                     /old_locus_tag="pgaptmp_001016"
     CDS             complement(54270..54596)
                     /gene="yciH"
                     /locus_tag="ACHKNP_RS05080"
                     /old_locus_tag="pgaptmp_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HGU4703227.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(54596..55333)
                     /gene="pyrF"
                     /locus_tag="ACHKNP_RS05085"
                     /old_locus_tag="pgaptmp_001017"
     CDS             complement(54596..55333)
                     /gene="pyrF"
                     /locus_tag="ACHKNP_RS05085"
                     /old_locus_tag="pgaptmp_001017"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGU4703228.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(55525..56694)
                     /gene="lapB"
                     /locus_tag="ACHKNP_RS05090"
                     /old_locus_tag="pgaptmp_001018"
     CDS             complement(55525..56694)
                     /gene="lapB"
                     /locus_tag="ACHKNP_RS05090"
                     /old_locus_tag="pgaptmp_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HGU4703229.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(56701..57009)
                     /gene="lapA"
                     /locus_tag="ACHKNP_RS05095"
                     /old_locus_tag="pgaptmp_001019"
     CDS             complement(56701..57009)
                     /gene="lapA"
                     /locus_tag="ACHKNP_RS05095"
                     /old_locus_tag="pgaptmp_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HGU4703230.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(57158..57922)
                     /gene="pgpB"
                     /locus_tag="ACHKNP_RS05100"
                     /old_locus_tag="pgaptmp_001020"
     CDS             complement(57158..57922)
                     /gene="pgpB"
                     /locus_tag="ACHKNP_RS05100"
                     /old_locus_tag="pgaptmp_001020"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HGU4703231.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            58092..58682
                     /gene="ribA"
                     /locus_tag="ACHKNP_RS05105"
                     /old_locus_tag="pgaptmp_001021"
     CDS             58092..58682
                     /gene="ribA"
                     /locus_tag="ACHKNP_RS05105"
                     /old_locus_tag="pgaptmp_001021"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HGU4703232.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(58746..>60716)
                     /gene="acnA"
                     /locus_tag="ACHKNP_RS05110"
                     /old_locus_tag="pgaptmp_001022"
     CDS             complement(58746..>60716)
                     /gene="acnA"
                     /locus_tag="ACHKNP_RS05110"
                     /old_locus_tag="pgaptmp_001022"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HGU4703233.1"
                     /translation="AMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHG
                     VVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQV
                     ELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAA
                     SNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKK
                     AVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPL
                     PDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLA
                     AEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTR
                     SDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKP
                     DSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLP
                     DSDVVSIYDAAMRYKKEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERI
                     HRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQE
                     VVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        14627 a        15970 c        15023 g        15096 t
ORIGIN      
        1 tatcgatttt atctgtacgt ccctgattgc ggttattctg acgattaagc tatttttgct
       61 gattaatcag tttgaaaaac agcagattaa aaaaggtcgc gatatcacca gtgcgcggat
      121 tatgtcgcgc attatcaaaa tcaccattat tgtggtgctt gttctgcttt atggcgaaca
      181 tttcggcatg agcctttctg gcttgctgac ctttggtggt attggtggtc tggctgtcgg
      241 tatggccggt aaagatattc tgagtaattt cttttccggg atcatgctct atttcgaccg
      301 tcctttcagt attggcgact ggatccgttc accggacaga aatatcgaag gtacagtagc
      361 ggaaattggc tggcgaatta ccaaaattac gacctttgat aatcgtccat tgtacgtacc
      421 gaactcgctg ttttcgtcga tcagcgtaga aaacccagga cgaatgacca accgccgcat
      481 taccacgacc attggtttac gttatgagga tgcggcaaaa gtgggcgtta ttgtcgaagc
      541 cgtacgtgag atgctgaaaa atcacccggc catcgaccag cgacaaacct tactggttta
      601 tttcaaccag tttgccgact cgtcattgaa tattatggtt tattgcttta ccaaaaccac
      661 ggtatgggct gaatggcttg ctgcacagca agacgtttat ttgaagatta tcgatattgt
      721 gcagtcacac ggcgcggact ttgccttccc cagccagacg ctgtatatgg ataacattac
      781 accgccggaa caggggcgct aaatcagaca tcactggcgt tatccacaag ggtaacgcca
      841 gttcccatca catcaatgct tcacaatata catagtccgg ctgtacgcaa catcttccgg
      901 gttattaatg ggataacctt tcagccacgg cttgattaat cgtccattgg tatattgata
      961 aattggtgca atcggtgctt gctccatgag gattttttct gccgcgttgt aatcggcatt
     1021 acgcgcttta acggtatttt ccatcgatgc ctgggccaga actttgtcgt atgccggatt
     1081 gttaaagcgt gaaatatttc ctgaatgcgt tgacgttaat aatgtcagga aagtggaggg
     1141 ttcattataa tcccccaccc atgaggcgcg gataacatca aaattgccgg tgttacggct
     1201 atcgatatag gttttccatt cctgattttg caatttaaca tctacgccaa ggttcttttt
     1261 ccacatcgat gctacagcaa tcgcaatttt ttgatggttt tctgaagtgt tatacaaaag
     1321 cgtcagcttc agcggttttt gcggaccata accagctgcg ctcaacaaag tttttgcctg
     1381 cgcattcagt tcttcctgac tcatttgttc aaacggcgaa ggttccggcg taaatccggc
     1441 ggtaacatct ggtgtaaaat gccacgctgg cttttcgccc gttcccaaca ctttttcggt
     1501 catcaggcgg cgatctatcg tcatacttaa tgccaggcga acgcgctgat ctgccgtcgg
     1561 ccctttttgc gtgttaaacg cataataata ggtcccgagc tgtggcggcg tataaacctg
     1621 ccccggaata tccttcaaca gcttctgata catatttttc gggaaggatt cggtgatatc
     1681 aatatccccc gcgaggtaac gcttagtggc tgcggattcc tgattaattg gcaggaaggt
     1741 cactttttgc agtaccgttt tggcgttatc ccaataatgg gtattcggta ccacgaccag
     1801 tttttcattg actacgcgat ctttaagaac ataagcgcca ttgccgatca gatttccggg
     1861 tttcgtccac tctttaccgc tttctacgtt ggctttttgc accgggaaga aggcaaagtt
     1921 agcggttaaa ttcacaaacc acggcaacgg tttatcaagc tgaattttca aagtatgggc
     1981 atcaactgcg gtgacgccaa gctggtctgg cgtagcttta ccatcaataa tcgcctgtgc
     2041 gttgttgatt cccgccagcg cggcaaacca tgcaaatggc gatagcgttt tcgggtccac
     2101 cagacgttgc cagctgtaga caaaatcttg cgccgttacc ggtgtgccat ccgcccattt
     2161 tgcgttatcg cgcagggtaa aagtccagat acggttgtca ttacttttcc actgagtcgc
     2221 aacgccgggg acaatctccc ctttctcgtt ctgattcacc agtccttcaa acagatcgcg
     2281 aatgacctga atctctggca ggcccacggc tttagcggga tccagcgacg caggctcatc
     2341 tttaatgtgg cgcaccagct cctgcttctc tgccagtact gtgccgctcg gaacttctgc
     2401 agcatacgaa agagaaatgc tgctgaccaa cagcgcacaa cacgtgactg aaacagagtg
     2461 cttcataagt ttccctctaa ccgacattaa aattgatgcg taattatttg tttcgcctta
     2521 aagaaatgca aacaacttcc atcaaaaaag tgacacagga agcataaaat gttttatgta
     2581 tcaatatatt aaatcagatt tagtgtaaat aatcgacgcc aaaatgcggt aaagcacata
     2641 gaacaccgaa cactacgttc aggcttcgca cgttcaaacc agttttcggt atcataatca
     2701 gcatctgtcg ccattgtacg aagaataaaa aataaccaaa acgttgaaag gaactgagaa
     2761 tacgttaaga gagaaattaa attaatgtat cagcaagtag tgctaaacag cacctttata
     2821 cttcagccta tctataaata atgagaatgc cgagccagca tgtcgacggt gtggatagta
     2881 caggtgatga ccgggtaaat ctggtgtgaa tttatccaaa acacgtatca gttttttttc
     2941 tttaatagcc cgctcaacct gatcataagg tagatacgcc aatccatgcc catcaattgc
     3001 agcatcaatg atcaggtcta tcgtattcag taaaagctga ccttccatgc gaacacgaac
     3061 ttcacgtcca ccgcgtatta atctccagcg atttgctgta cccgatgtgg gaagatacaa
     3121 attaattgcc tgatgatcta ttaattgtga cactgacgtt ggaacacttc ggcgagaaaa
     3181 ataatccggt gagccaacaa tagccattgg tatatctggc ccgattcgaa tggcgatcat
     3241 atctttatcc atctccccac ccagacggac gcctgcatca aaacgttcag aaacgacatc
     3301 ggtcaaacca taatcaatgg tgagctgaat atcaatttca ggatgcgatt tcaggaatgt
     3361 gcgcattgct ggtaacaata tcgtttttgc tgcatgttct acagtagtaa tacgtattgt
     3421 cccggatggg cggttctgca gctcgctcag ggatgccagg gctgaatcta tgtcatgcaa
     3481 catagggcca agaacagaca aaagatgctc gcccgcttca gttggaacaa cgctgcgcgt
     3541 ggttcgcgtc agaagccgca atcccaatcg ttcttctata cgacgcacta tctggcttaa
     3601 agctgactgc gccatgctca ggcgggctgc tgcacgagtg aagctacgct cctctgcaac
     3661 gacgacaaac gccatcagat cagcgatttc ttcacgcttc attcagtatc tccatgcaat
     3721 ctattaatca tttaaatgat aggtctaatc atgattttgg gtctaatcaa cttaatgatc
     3781 aatatctata gtgatatcac caaccgttaa aacgaaccga caactccatg aggaacggaa
     3841 tgatgaataa taaagtcagc ttcactaaca gcaataatcc aaccatctct ttgtctgcag
     3901 tgagctattt ccctccaaaa tttgatgaaa cccgtcagta tcaggcaatt gtggtatctc
     3961 acccaggtgg gggcgttaaa gaacaaacgg ccggaaccta tgccaaaaaa ctggcggaaa
     4021 agggatttgt cactattgct tatgacgcat cttatcaagg tgaaagtggc ggcgagccgc
     4081 gtcagttaga aaacccatac attcgtactg aagacatcag tgccgtgatt gattacctga
     4141 caacgctttc ttatgtcgac aatacccgaa ttggtgcgat gggaatttgt gccggtgcag
     4201 gatatacagc caatgctgca attcaggatc gccgcatcaa ggccatcggt accgttagtg
     4261 cggtcaatat tggttcgata ttccgtaatg gttgggaaaa caatgtgaaa tctattgatg
     4321 ccctgccata cgttgaagcg ggttcaaacg ccagaaccag cgatattagc agcggagaat
     4381 acgccattat gccactggca ccaatgaagg agtccgatgc gccgaacgaa gaactgcgtc
     4441 aggcctggga gtattaccac acccctcgcg cgcagtatcc aacagcaccg ggttacgcta
     4501 ctttgcgcag ccttaaccag attattacct atgatgctta ccatatggcg gaagtgtacc
     4561 tgactcagcc gacgcagatt gtggcgggca gccaggcagg gagcaaatgg atgagtgacg
     4621 acctgtacga tcgagcatca agccaggata aacgctatca cattgttgaa ggcgcaaacc
     4681 atatggatct ttatgacggc aaagcctatg tggctgaagc aatttcagta ttagctccgt
     4741 tctttgagga aactctgtaa tgaaacatat tcaaatccgc aatagtgata tggcctggca
     4801 tattgctgcc aatattcagt ttccaccaaa ttttgatgag tcaaaacagt atccggcgat
     4861 tatcagcgtc catccttttg gcagttgtaa ggagcagacg tctggcaata tctatggcaa
     4921 agcattggct gaaaaaggct atctcgttct cgcttatgac gccagtttcc agggagaatc
     4981 tggtggggag cctcgctgga tagaagatcc gacacagcgc gtagaggata ttagccgtgt
     5041 tatcgattat gcagtaactc tgccatatgt taatgctgaa cgtattggcg tgctgggagt
     5101 gtgtgggggt ggtggctatg ccattaatgc cgcgctgaca gaaaaacgaa ttaaagcagt
     5161 agtgagtatt acgggtgtta atatcgggcg tctttttcgg gaaggtttca gcaattacga
     5221 tccgattggc gcattgaatg caatggcgtc gcaacgaacc aaagaggctc gtggtggaga
     5281 attacagatc aacgaattac ttcccgccag cctggatgca gcaaaagccc atggactgac
     5341 cgaacgcgat gtttatgaag caactgacta ctataaaaca ccacgcgggc aacaaccggg
     5401 aggtgcgaca aaaatgttgt tctcacatgc acagaaaacg ctggcctggg atgccttcgc
     5461 atttactgaa gtgttactga ctcagccggt gatggtagta gttggtgaaa aagtcggtgc
     5521 cttcggtgct tatcgtgatg gactggaagt ctatggccgg gcgatggttt cgcaggatcg
     5581 tcagttagtt tcattacccg atttctcaca ttatgaactg tatgacaaac cggaagcagt
     5641 tcagaaggcg ctggaaaaag ttattccttt tttcaacacc catctgggat aagtttcatg
     5701 agaaatatta tgaaaaatgt actgattctt ggtgctggcg gtcagatagc ccgccatgtg
     5761 attaatcaac tggcagataa acaaaccatt aaacaaactt tatttgcccg tcagccagca
     5821 aaaatccaca aaccctatcc aactaatagc aagattatta tgggagatgt actgaatcat
     5881 gcagcactga agcaggccat gcagggacag gacgttgtgt atgccaacct tacgggggaa
     5941 gatttagata ttcaggcaaa ttcagtgatc gctgcgatga aagcctgtga tgttaaacgt
     6001 ctgatctttg ttctttccct cggtatttat gatgaagttc caggaaaatt tggcgaatgg
     6061 aataatgcag ttattggtga gcccctaaaa ccattccgca gggctgcgga tgcaattgag
     6121 gcttctggac tggaatatac aattcttcgc cctgcctggc tgactgatga agatattatt
     6181 gattatgagc ttacctcacg caatgaacca ttcaaaggta cgatcgtatc acgtaagagt
     6241 gtctcagcat taatcacaga tatcatcgat aaacctgaga aacacattgg tgagaatata
     6301 ggtatcaatc aacccgtgac tgatggtgac aagccattat tcatgtaaat caattacata
     6361 gttaccatcg taatgtacta tgtgcagtac gatttattaa acttattaag gtgaactgat
     6421 caatccgatg cgattaacat gtgtagaacc agtaagaata aaatacgcca tgtatataac
     6481 attacgactt acatctaagt cagcataatt ctaatatctg cacatcaata aaaatgcaaa
     6541 caacttccgg caaaaaagtg atacaagatc gatgacatag cgttgaagaa aaggatttga
     6601 taatccgcaa aatttgcaca acactgcctg taatacttcc tgaaccagag acgatcatga
     6661 ccgtaaaccg cccacgcgcc gaacgcggcg catttccgcc cggaacagaa cattacggac
     6721 gttcattatt gggcgcgccg ttaatctggt ttccggccac tgcagccagt cgcgaaagtg
     6781 gtttgattct ggctggcact cacggtgatg aaaactcttc ggtcgtcacg ctctcctgtg
     6841 ctctgcggac tttgacacct tctttgcgcc gccatcatgt agtgctgtgt gtgaatcctg
     6901 acggctgcca gctggggtta cgggccaatg ctaatggtgt ggatttaaac cgaaactttc
     6961 cggcggcgaa ctggaaggaa ggtgaaacag tttatcgctg gaacagcgcc gctgaagaac
     7021 gtgatgtcgt attgctgaca ggtgacaaac ctggctctga acctgaaacc caggcgctgt
     7081 gccagcttat acaccgcatt caacccgcct gggtggtctc attccacgat ccgctggcct
     7141 gtattgaaga tcccagacac agcgaattag gcgaatggct ggcccaggcg tttgaattgc
     7201 ctctggtaac cagcgtcggt tatgaaacac ctggctcttt cggtagttgg tgtgcggatc
     7261 tcaacctgca ttgcatcacc gcagagtttc cgcccatctc ctccgacgaa gccagcgaaa
     7321 aatatctctt tgcgatggca aacttgctgc gctggcatcc taaagatgca attcgcccgt
     7381 cgtgaactga agcgccggtt ccacatctac cgccagccag gtcggtccgt caagatcgac
     7441 gaaactgacc tgcggcacca gcggtaaagc ggcgctaatg gcccgagagg tacacaacat
     7501 gcagcccagc atcagacgaa aaccttgtgc acgcgcttca gtcgccagcg ccagcgcttc
     7561 cgtcagacca ccggttttat caagcttaat gttaaccatc tcatagcgcc ctttcagcgc
     7621 cttcagattg ctacgtgtat gacaactttc atctgcacaa atcggtaacg gatgaataaa
     7681 attctccagc gccgcatcgt cctgcgcagg aagcggttgt tcaagcatcg caacgcctaa
     7741 atccgccaat agctggcaac gcgccgccaa cccttctgca cgccaggatt catttgcgtc
     7801 aacgatcagc gtcgcatcgg gcacagctgt gcgaattgcc accatccgct cactgataag
     7861 atggttatcc agcttcactt tcagtaattt cgcgcctgcc tgccagagtg ttgatgcact
     7921 attggccatc tgatcaggcg taccgatgac taccgtctgt gcagtggtga ctgtcccggg
     7981 aagcgttatg ccgatcaaat cagccagcga ttgctgctgt tttcgcgcgg ccagatccca
     8041 caatgcacaa tccagcgcat tacgtgctgc gccggcaggg agaatttttt gcaactcctc
     8101 ccgtgtcagc cctttctcta attgcggcac gacgctcata atttgcgcca ttaccgaggc
     8161 atcactttcc ccataacgcg gatacggcgt gcattcgccg gtgcctttaa taccctcttc
     8221 ttccagttca accaccacca cgcgcgcttc actgcgactt ccccgggcaa tcacaaacgg
     8281 ggtatgtaag ggccaggctt cctcaaatac cttaacggtt ctcatcttta ctccttgttg
     8341 tcgcggtggt ctgcggatta agcgcaaata gcgtttgctg tgttattgac agttagcata
     8401 aactaggtgt gacgttaact atatgtaaac aggaaagata atcatgtcac aaaccgttca
     8461 tttccagggc aacccggtta cagtcgccaa ttccatcccg caggcgggta gcaaagcgca
     8521 gacttttact ctcgtggcaa aagatctgtc tgacgtcacc ctcggtcagt ttgcgggtaa
     8581 acgcaaagtg ctgaacattt tcccgagtat tgataccggt gtttgcgccg catcagtacg
     8641 taagtttaac caactggcaa ctgagatcga caacaccgtt gtgctgtgta tctctgccga
     8701 tctgccgttc gcccagtctc gtttctgcgg cgcagaaggt ctgaacaacg ttatcaccct
     8761 ctccactttc cgtaacgctg aatttctgca agccttcggt gtggcaattg ctgatggccc
     8821 actgaaaggt ctggcagcgc gtgccgttgt ggttattgac gaaaatgaca atgtgatttt
     8881 cagccagctg gtggatgaaa tcaccaccga gccggattac gaagcagctc tggctgtact
     8941 gaaagcataa gttaatatgc ctgatggtgt tgcaccatca ggcatattcg cgcttactct
     9001 tcgttcttct tctgactcag accatattcc cgcaacttat tggcaatcgc ggtatgtgaa
     9061 acgccgagac gttttgccag tttgcgcgtg ctgggataat tgcgataaag ctgggttaat
     9121 accgagcgtt caaaacggct ggtgatttcg tccagcgaac cttccatcgc atcttcgccc
     9181 acggctaccg ttgcggcgtc ataatccggc aacaaaatat cctgtggacg cagctcataa
     9241 ccgtccagtt gtgtcagtgc gcgatagata gcgttcttta actgccgcac atttcccggc
     9301 cacgcataac gcgtaagtac agtattcagg tcagcggcca gtttcggacg cggcacgccc
     9361 tgctcgtcgg caaagcgggc gacgaacagc tcagttaacg gcatgatgtc ctgcggacag
     9421 tcacgtagcg gcggcagatt gagcgtcaac acgttcagac gataatagag atcttcacgg
     9481 aacatgcctt tttgcaccag ttcgaccaga ttcttctgcg tagcgcaaat cacccgcaca
     9541 tcgacatgca cctcatggtc ttcgccaacc cgacggaaag tgccatcatt aaggaaacgc
     9601 agtaatttcg cctgcatccg tggtgacatt tcccctattt catccaacag caccgaacca
     9661 ccgttcgcct gctcaaagaa tcctttcttc ccttccggag catgaccaaa cagttcactc
     9721 tcgaccgcat cttccggtat agacgcacag ttcagcgcca ggtaaggttt gcccgctctg
     9781 gggcttgcct gatggcaggc gtaggcaaag agatctttac ctgtacctgt gtcacccgta
     9841 atcagcagcg gcgcgcttag catcgccagt ttctgcgcct gttcgacaac atgcttcatt
     9901 ttcgggctga cggcgacaat ttgactgaag gcgctgacgt cctgggcggc gacattttgc
     9961 aactggcggc ccatacgaat cgttgatcgc aacatcacca ccgcaccggt caggacgtgt
    10021 tgatcatttt catcctgaag ataaacaggc gtaatctcca tcaggaaatt ctgcccatta
    10081 ataacgacat gctcgttatg ggaatcttgc ggttcgcttt ccagccaacg taaaaaatta
    10141 aagccgttaa tcaattgtgc ggcggtatgg ttgcgcaggc gatccaattt ttgcgcaaaa
    10201 agctgacagc tcgccgggtt cgccatatcc actttgcttt tcatatcgac agagagcaca
    10261 ggttcgggca acgcctccag taacgcgctc aacgccagat gctcacgttc ggaaggcatc
    10321 cacgggacag tacgcacatc ggtaacacct gcaatacggc gtatttcggc catcagactg
    10381 ctgaaactct caaactccag ttcagcaaaa ttgaggtaga ttcgcccaat gggatcaatc
    10441 tcaataccgc gtaaatcaat gcctcttagc acgagtagat cgagtaattc gcgggtcaga
    10501 ccgagtcggt cttcacaaaa gacttccaga cgcatgggaa ccttcacctg aaaaaagaac
    10561 aattaatatg atgatatgac actatttgga tagcaggaag gtttctgtca acaatcattg
    10621 acacaaagac ggaaaaacgg gcacttcggc gtaaagatat ccggtcagca cccgatattg
    10681 cattattttt cgctgggcgt tttgcctttt tgcagcgttt ctttcacctg accgataagc
    10741 tgacgacgga aatccccgag gcgaggtttg tcatcgtcaa tccacggcag cgggcggcaa
    10801 agctccatag ctttaatccc gagtcgtgcc gtcagaagtc cagcaccaat tccctgagct
    10861 gcgcgggtag acaaacgagc agcgagatct tgcgacatcc agtccatccc cacttcgcgc
    10921 accagttcgc tggctccggc aaaagcgata ttcagcaata ccagcttaaa cagacgcaaa
    10981 cggctgtaat accccagttc aatgccatac agcgtggcga tgcgattaat taaacgcaga
    11041 ttgcgccagg cgataaacgc catatcgacc aacgccagcg ggctgaccgc aatcatcaac
    11101 gttgattccg ccgccgaacg gctgatttcg cgccgcgcct gggcatctaa aactggctgg
    11161 accaaatgcg catacaaact gaccacttca cggtcgtttt gcgtttcatg gattgaggca
    11221 taccagcgtt gcagcgctgg atgagactga tcaatacccg cctgctgcgc cagtttttcg
    11281 caaaatgcgc ggcctttgcc cgtgccgtgg ctgtgcaaca aatcgcgcgc ttcgtcgcgt
    11341 tcatgggcgc gctgtcgcaa gcgccataag cgccgccact ctgttaccac agaacctacg
    11401 ccagcgccga taatcaatgc cccagcggca catccaccca gcgccaccca gtcctgagtt
    11461 tgccaggcat tcattgtcca ctgtacaccc tgcccgacaa cgcttgcgcc aaacagagcc
    11521 agcccgccca tcaccatttt gcgccacagg ctgcgtttcg gacgtaacgc tgcatccatt
    11581 accgcttcaa cttgcccctc ttcttcaggc gcttcatcga gcgtggccgg ggcaaaattt
    11641 tgcgcctgat tttcgtcaaa ggtctgctgc gccctgaatt ttggattctg atcgacctcc
    11701 agcggaccgt cgaaatcaat acgtggtttt aacggttcgg tcatcgcaat ttatctccta
    11761 ttaaaaattc aagcgcggca tcaagacgaa tatgcggcag cggtttgtcg acatccatca
    11821 cctgcgggcg aaacgcttca aactggaacc cttgcttgtc ccagaacgcc tgaccgggca
    11881 aacgcgcagg aacttcaccg ggataaacgg tgagtggcgc accatcgctg agccggttac
    11941 cgcgcaatgc cggaattttc tcgccgttga cgtcaataat gccgctggtg gtcgcctgca
    12001 ccgaggccag ccccaggcaa tccatactaa tcccttcaaa cgccgcattt tgccaggcat
    12061 cctgaatcag ttgttgcagt aatgaaacca tattggcgtg ctgatcgatg gtcacatggt
    12121 ccgctttagt ggcagcaaac aatagcttat cgataaccgg cgaaaacaaa cgccggaaca
    12181 aggtacgctg cccgtagtgg aaactttgca tcagctgcgt cagtgccaga cgcatatcat
    12241 taaatgcctg tggcccactg ttgagaggtt gcaggcaatc aaccagcaca atctggcggt
    12301 caaagcgcag aaaatgattc ttatagaacc ccttcaccac cttctcgcag taataattaa
    12361 accgctcgcg cagcattccg gcattggtgt gcttatcggc ctgcgccagt ttggactcgc
    12421 cccaggcatc gacatccggc cacgggaaga attgcagcgc gggcgcacct gccatatctc
    12481 ccggcaagac aaagcgccca ggctgaataa agtgcagccc ctgctgttta cagtggtgga
    12541 gataatcggt ccacgcggcg gcaatgtctg ccagccggtt ttcgtcggca ggcgctagcg
    12601 ggtccagccc ttcgcacatc attcgccatt tgaccgacca ttcgccgcgc tgaccattga
    12661 gtaagcccgt catctggcgt gaccagctta aatagtcctg cgccagcatc ggcaggtcga
    12721 gcaaccattc gccgggataa tccacaatct ccagatacag cgtggaggtg tccttgaagt
    12781 ggcgtagcag cgaatcgttc gatttaaaac gtagcgccag gcggatttca ctgacgccgc
    12841 gcgttggcgt cggccaggcg ggaggatcgc catataactg cgccagtcct tcatcatatg
    12901 tgaagcgcgg aatgccaaag tcacgctgag gaatgcgttt tacgcccagc aggcgctctt
    12961 cacgcaccgc gcttaacagc ggcaagcgtg ctccggcgtg aatattgagc aactgattga
    13021 ccatcgcagt gataaacgct gttttgccgc tgcggcttag tccggttacg gcgaggcgca
    13081 gatgtctgtc gacaccccga ttcaccagcg cattaagttc atttttaagt cgcttcattg
    13141 ctgtcctgtg ctgctctgaa gaccagaaaa aaatcattcg taaggatatg aatgtggggg
    13201 ccaggccatt gattatcaac tgcccgtgtt gtcatcgacg ggcgaactcc ataagatgtg
    13261 cagcattcac gttcggacgg agttcctgac ctatgtcccc tactatttat gatattgcca
    13321 gggttgcagg cgtatcaaaa tccaccgtat cacgcgtgct gaataagcaa actaatatct
    13381 ccccggaagc gcgcgaaaaa gtgttacggg ccattgaaga attacagtat cagccaaaca
    13441 agctggcccg cgcgctgacc tcttcgggtt ttgatgccat tatggtgatt tctacccgtt
    13501 cgaccaaaac tacggcgggt aatccgtttt tctctgaagt tttgcatgcc atcaccgcca
    13561 aagctgaaga ggaaggtttc gacgtgatat tgcagacgtc gcacaatccg gcagaagact
    13621 tacaaaaatg tgaaagcaaa attaagcaga aaatgattaa aggcattatt atgctaagtt
    13681 cgccagcgga tgagtcattt tttgcccaac ttgataaata cgatattcct gtcgtggtga
    13741 ttggcaaagc tgaaggtcaa tatgcccatg tttattctgt cgataccgat aattttggcg
    13801 acagcattgc gttgaccgat gcattaattg aaagtgggca tcaaaatatt gcctgcctgc
    13861 atgctccgct tgatgtccat gtttcagtgg atcgggtaaa tggttataag cagagcctgg
    13921 ctgcgcataa tattgcagtg cgtgatgaat ggattgttga cggcggttat acccatgaaa
    13981 cagcactgca agccgcgcgg gaattattaa gccagtcacc gttgcctgag gcagtgtttg
    14041 ctactgacag cctgaaatta atgagtattt atcgtgcggc agcagagaaa aatattgcta
    14101 ttccgcagca gttagcggtg gtgggttata gtaatgaaac gctgtcgttt attttaacgc
    14161 ctgcgccagg cggtattgat atcccaacac aggagttagg tcaacaaagc tgtgagttat
    14221 tattccgctt aatttctgga aaaccgtcac cacagaatat taccgttgcc acgcatatga
    14281 cgctgaaata aataccagct accttgcctg aatgccggat gcgatgctag cgcatcttat
    14341 ccggcctaca tttatagttc ggataaagta ctcgtgccgc atccggcaat ttagcccatt
    14401 atcagaacga gtaatttacg ccgacacctg cataatggaa tttatcgctg tcgccttcgt
    14461 cgtgatcctg ccactcaaac gcgtattcca gcgaaacgga aagcccgttc tggaaatcat
    14521 aaccgtaaaa taaacctaca cggttaaaat catggccttc acgttcaata tcatcctgcc
    14581 agtcccagtt actccagcga tccagcccaa tgcgcgtata cggcgtcacc gagtggttgc
    14641 cgagcgtcag cggcaaatag gcgcgaattt cttgcgtaga aaactcaccg ttattgcggc
    14701 tgtcgtccat attaaagccg cgctcgagat aatagttcac tcgcaaggca accgtttcgt
    14761 tgaaggtata ttgcaggcct gtttccgttt cgactcgggt atcagcgtaa ccggtcgtgt
    14821 tcagatcgtt ggcaaattta tacatcgaca accaaccgtt gaaacgtaaa tcgtcagtca
    14881 gtttcacatc ccagtctggc gcgattttcc agcgctgcat attcgccgtg tctttacccg
    14941 gttcatcaac gtagtgataa ccataattac ggaaaccgcc ggtcaggccg aaactgaaat
    15001 catcgttttc gaggaactga taatgcacct ccagctccgg gcgatcaaac cacgttccac
    15061 gtttacccgc gctataatct accggccctt cctgataata ggccagagca attctccacg
    15121 gcccgttggc ggcattaaaa tagactgaag gctccgccag cccatccata tcttcgccat
    15181 aaccctcgac gttttctatt tcgtacatcg cgccgatatt aaagtgccag tcgttccttt
    15241 cctcggcctg tgcgcaggcc attcccgcac acatcaccag tgcggtacag ggtaataact
    15301 ttttcattat tgttccttgt attattctgg gggaatgcct tccccctgta tttagcgaat
    15361 tgctatttcc gtttctgcat caaagaaatg acatttcgtc atatcaaaat gaatagtgat
    15421 attttctcct gcatgataat catttaacgc accagcacgg accactaact cgtgcccccc
    15481 aaccgtggtg tagagcataa attccgcacc ggttaattct gccacgctaa ttttggcgga
    15541 aatgttattt tcctcttgcg cgtccggatg aatatcttcc ggacgtattc ccatcacgat
    15601 gggcttatgc aaacttccct gtgttttcag aaccgctaat ttctcttcgg gaatggttaa
    15661 tttaagcgtt tccgtaacga atttatcgcc atcgatcgtg ccacgaataa aattcatcgc
    15721 cggtgatcca ataaatccgg caacaaacat attcgctggt tggttataaa ccgttttcgg
    15781 cgcaccaact tgctgaacaa tcccatcctt cataatcaca atccgcgtcg ccatggtcat
    15841 cgcttcggtc tgatcgtggg tcacgtagat catggtggta ttcagcttct ggtgtagctt
    15901 gctgatctct gcgcgcattt gcacgcgcag cttggcatca aggttagaga gcggttcatc
    15961 cattaaaaac acgcccgctt cgcgcacaat cgcccgcccg agcgccacac gctggcgttg
    16021 cccgccggaa agcgcgcccg gcttgcgttt caggtactca cgcaggccga gaatttgcgc
    16081 cgcccagttc acccgctcat caatcacctc tttggcgatt ttttgcatct tcaggccaaa
    16141 cgccatgttg tcgtaaaccg tcatatgcgg atacaacgcg tagttctgga acaccatcgc
    16201 tatattgcgt gctttggctg gaacgtcatt cattcgtttg ccgtcgatca acagatcgcc
    16261 gccgctgatc tcctcaagcc cggcaatcat gcgcagggtg gtcgacttac cgcagcccga
    16321 cgggccgaca aacacgatga actctttatc ggcaatttcc aggttgaagt ccttcaccac
    16381 atgcacctgg ttatcgtaga ttttttgaat atgttgtaac gaaagctgag ccatgttgat
    16441 tcctttgcta tacgttttgc cagaaggccg ataaccgcga ccaggtcaga gagtccgttg
    16501 aaggcaacag taattgcgcc ccggttaagc ccgcgccgat ccccaccgag cgcataccgc
    16561 tggcgttaat ggcgtcaatg cccgcctgcg catcttcaat gccgatacat gcctgcggcg
    16621 gcacgcccag cccggcacag gcggcgagaa agatttccgg gtccggtttc gagtttttaa
    16681 gttgggaagc atccgcgcag aaggtgaaaa actcgcgcag ctccagcgcc gctaaaatcg
    16741 tcggcgcatt cagggagaca gaagccagcc caaccgggat ctgctgtgca cggagatctg
    16801 ccagcaaaga gcgaatgccg ggtagaacag cgctgaccgt caactcgcgc agtgagtgga
    16861 catagagcag atttttgcga tacgccagtt gcgccctctc ctgcgagtta aagtcgccct
    16921 ctttgccccc gtgttgcaga atgcgccgca gagactcatc gcggctgatc ccttttaggg
    16981 attcgttaaa ctgcgcatca atgctgatgc caatttcagc ggcaatctgc tgccacgcct
    17041 ggaaatgcag atgcgcggta tcggtgatta caccatccag atcgaaaatt accccttgca
    17101 gtttcattca tcctcctgat gcgtggtagc ggtcccattg aagggcaaaa taaaatcacc
    17161 caaacagaaa acagattctt cagccacgga tataagctga ccgttcaaac gcagtgaaac
    17221 gggcgcagaa gttcgaatcg caatacgctg cgcgtcaaga gtgacctgta attcgcagcc
    17281 ctgccagaac agagggaaag acaactgttg ccactgctca ggtaacgccg gattgagatg
    17341 caattcaccg tcacgcacgc tcaccccggc aaaaccctga atcgccccca gccagatagc
    17401 gccagttgcg gcagcatgga taccatcatc acaactatgc ggatcagcac caagatcgat
    17461 ttcagtcccc tcgcgccaga actgataact ttgggtcagc aggccacagc gtgcggcaac
    17521 aatgccgtgg attgctttac ttaatgacga gtcgtgaata gtgcgcggtt cataaaattg
    17581 cagattggca agacacgatg ccgctgagaa ctgctccggc agcatgtaat tgagcatcac
    17641 cacatcagct tgtttgagga tttgcatctc gttcacttct gcgcgtgaat aatccagcag
    17701 aatggtttgc ttccccgccg ccgctttgta tttcgccaga ttaatcgccg gcttagccat
    17761 aaacgaatca tcctgcggca aaacgccgtc gggctgaatt tctggcagcc gcagttcttt
    17821 aaggaacatt tcggcgcgat ggataaacgc atcgtcgcta cagccaaact ggcgggcaat
    17881 actcagcgcc tgctggacgt tgtaatacgc catatagctg gtgaaggcgt tattattgac
    17941 atgttcggta tattcgtcag gcccaataac atcatgaatt tccagacggt cgttaaccct
    18001 caccgcgcgg ctaatccaga actttgcagt ttccagaagt agcgccatgc cttcatgcgc
    18061 aatgaaactt tcatcccccg tggtctgcca gtattgaata accgcccagg cgatatcggc
    18121 caccagatga tgttccgcct gcgccgaggc cactttttgc cgcagcccgg tgcgaatgtt
    18181 aatggcggca aattccggcg tctcttcttc gccgctgcgc gcgctttccc acggaaatag
    18241 cgcgccctgc cagccgttgc gtcgcgcttt ctcctgcgcg cctggcaagt tgtgccagcg
    18301 ataacgcagt aaacttcggg caaccgtcgg atcgctaaac agatgaaacg gtaacaaaaa
    18361 tacttctgta tcccagaaaa cgtggccttt gtagccttcg ccggttaagc cttttgccgc
    18421 aatgctgctg cgctcgtcgt gggcaggcgt catgatgcgc agatgataaa gcgcataatc
    18481 taacgcttgc tgatcgtgcg cttcgccgcc atttaccgtg atacgacgtt tctgccacca
    18541 ttgacgccag ttttctgttg atactgcaag aagttggtcg taactctgct gcgcgcacat
    18601 ttcaagctgg cgaagcgacg cgctgcccca ctcgtctaaa acggcttgcc tgtcatcccg
    18661 ccagtcgatc cacaccagtt tttgcaacgt cactgtctcg cctgcatgaa gctgcgcact
    18721 ggtatgttgc tgcaaacggc gctctttggc ggtatagcat tgctgcacat caccgctcac
    18781 catacagcaa cagctgatgg ccacatcact gcgtccatcc tgggtggtgt agatcccctg
    18841 catcagatgc tgaccaaaca cccgcacctg ggtttcgtcg agatgttggc gaccgtggtt
    18901 ggtttgcgtg gcgtcgatac ctgttgaaat cagcactgac gcgtcggcgt ccagtggcgt
    18961 aatagtgatt tccagcacaa tgagcggcag ttggtctgcc gaaacaaagc gacggctggc
    19021 gatggtgtaa cctgtgccgt tgctggtacg ccagacaacg ctacggcgta attctccact
    19081 ggcaaagtca agttcacgct gccaggcttc gcgggataac gagaaaacct caccatttat
    19141 ggcaatctcc atccccaaga tatcaggcag gttcaccagt tcgttgattt caccttttcc
    19201 cgcccgatga tacagccccg ccagatacat ccctcgcgtc tggcgcgtgt aatcttcttc
    19261 atggctggcg cgaagcccaa gatagccgtt cccctgcgcc atcagcgatg catacttgtt
    19321 cagggtatgc tggctgaaat ggggttctga taacgttact ggcctggtca taattccaca
    19381 cgcgtccctg tttctgcgga ttgatacaac gccgccacca gttgctggat gatgtacccc
    19441 tgctcggcgt cggctatcat cacgggcttg ccctgtacgt gattgataaa ggcttccatg
    19501 ctgcgcaaat ggcggttgtc gtctgccagt tcccgttgca tcagcgtcat taattcaccg
    19561 ttgttatcgg tgtagatatg tgctggaaac agcgtcgcac cggctttatc accacaaaag
    19621 ctgacgttca taatcgactg ttcgcggatg ttgagtgcaa atgacgtttc cagccacaga
    19681 atgccgccgt tatgaaattc aatggtgcca aataacgaat cttcgacgct gtaagttgcc
    19741 ggatcccact cgccaaattg accgcagctc ttttgcgtgc cgatcttttg aaagctatgc
    19801 gcattcacgc ttttcaccgc cggaaagccc agcacataca tcgccgcatc cagcatatga
    19861 atgccgatgt cgataagcgg gccaccaccc tgcagttctt tattggtaaa gacaccccag
    19921 ccgggaacgc cgcagcgacg cagggcgcgg gcggtggtaa cgtaaatctc tcccaaaacg
    19981 ccgttggtca cctgttcacg cagctgttgc gtatcgagcg caaaacggtg gtgaaagtcg
    20041 taggccagca ccttgcccag tttgcgcgcg gtatcgcaca tttcccgcgc ctgttctggc
    20101 gtcatggcgg gcggtttttc gcacatcaca tggcagcccg cttccagtgc catcagggta
    20161 tgttcgtaat gaaaacggtt aggtgagcag acgctaacca catcaggttt caccgccagc
    20221 agcatggcct gcggatcgtc ccacacggat gcattaccgt atttatctgc cagcgcctgc
    20281 gcctgggaaa ggcggctgtc acagacagcc accagttcca gatcgttgcg ggtgcagtag
    20341 tacgaagcat gaaccttatc cgccacctgg cctgcgccta ttatcgcgac ccgcagcgga
    20401 gagcttgtca ttgcactttt cacaaagacc tcttagcagg tacgcaacca ggccaacgaa
    20461 tcacggtacg cctgggcagg ctcttccgcc cggatacgcc cttcgtacac tacatagccc
    20521 tgatagttat ccgcgcgcag ctgttcaaac agcgcgtgga aatccagggt gccgctgccc
    20581 ggctggtagc gatggttatc cgcaatatgc acatgaccga gcaggtcgcg gttgtcatgc
    20641 agcgcctgcg ccaggttatc ttcttcgata ttcatgtgat agaaatcgcc gataatctgt
    20701 acatgtttaa gatcgttttc gacgatgtaa cggcgggcat cggcgagggt gttgatcata
    20761 tgatcctgat agcggtttaa cggttcgaga tacaccacgg ttccggtacg cgcggcgacc
    20821 tgttccagta cgtgcaggga atcactcacc attttgcggt cgccgtccag gctacgcggc
    20881 gaggtcatcg gcggtaagcg gaaggtaaac atgccccacg ctgccggaac gacgatacct
    20941 ttacccccca cttctgccag cgcttcgaga atgcgttcga tctgctttaa gccattaaga
    21001 cgacgctctt cgataaagtc gccaatccaa ccgtcatagc caccgcaggc ggtggtcacc
    21061 ggtaaaccgg tttctttgat tgctgctttg acttcttcga gattgttgac cagcagtttg
    21121 ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaattt ctccagaatg
    21181 ttttccggaa agaatgcctg attctgtgtg cctattttca ttttttcatt cctttgaatt
    21241 aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac tgcatgtagc
    21301 tttccgggct cttggcaaag gtcactaccg ggtcgattaa atcttcgcaa ttgagataac
    21361 cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag cgcggataat
    21421 ccgggttcgg ttcgctgcac gcacgggaga aaacaatttt ggcgttattg aaatgcgctt
    21481 cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacataa gagatggtgc
    21541 cgccataagc aaggccacgc agcgccgatt gcagcgcgtc ggcgtaaccg ctggtttcga
    21601 taatcacatc ggcaccctgc ttgccggtca gcgttttgat ctcttttcct acgtcagtgc
    21661 caatgaggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag cgataggcga
    21721 taggatcgac gccaatcacc accgacgcgc cagcgcgttt cgccagttgg atggctattt
    21781 gaccaatcgc gccaagccct accaccacca caaaatcacc cacgcgcacg ttggcatcgc
    21841 gcacgccgct catggcaaac tgcgccgggt cgtaacaaac ggcgtttttc cacgagctgc
    21901 cttccggcat tttgcgcagc ttgtagttat tcaccgcgtt aatgatgacc gtctcggaga
    21961 gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc ggtaacgtcg ctgccgcact
    22021 cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca atgccgcgcg
    22081 gcgcatctgc cggacgcgga gtgaacatct gccattcgcc gttaaagtct tcatcgataa
    22141 acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg ccgaaacgca
    22201 cgcggatttt cacttcattg gctaaaatcg cccgatcttc atactcaacc agcgcagcaa
    22261 cacgcggtgc tgtggctact aactttttca tttaaaactc cttgtaaaga aactggccgc
    22321 tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc gaaagcgcat
    22381 acataatcac caccggcaat gcggtcacca gtgaggccgc catcatccgt ccccagatgt
    22441 aatctggcgt actgaacagc gcgttcaggc ccaccggtaa ggtgaaattg ctggcgctgg
    22501 agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag tagacgaaca
    22561 cggatatcag cccggacatc gccaacggca cggtgatgcg gaagataatt tgcagacggt
    22621 tgaggccatc catcatcgcc gcttcttcga tctcatcggg gatggtgtcg aagtagcttt
    22681 tcagcataaa cacggcggtg ggcagagttt gcgtcaccat ggtgatgatc agcgccatct
    22741 cggtgtcgta aatgcccagc gcggtgatga ttttgaacag cggcaccacc agcaaaatgc
    22801 cagagaacat atacaccgtg taaaagctgg cattgatggt catccgacct ttaaagcgca
    22861 ggcgggaaag cgcgtaagcc ccaagaatgc cgaggaatac cgccaccacg gatgaaacca
    22921 ccgacaccac cagactgtta cggaagtagt cgacaaacgg gaaaatcatc gggttaaaaa
    22981 tgtcgacgta atgctccagc gtccactgct gcggcagcag cgtaggatgc agtgagatcg
    23041 cctctttcgc gcccttgaac gaggtcatca gcatcacaaa aaatggaaac agcgtgatga
    23101 tgagaaacag cgccagcccg cagtaaaaac cgatgcgact gagtgtgcgt ttatttgttg
    23161 ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat gatgaagagc
    23221 accaccgaga tcgccgccgc ttttcctaaa tcattaaagg caaaggcggt tttgtagaga
    23281 tatacaccga gaatatcgac cttggtcgtc agcaaataaa catccgcgaa catgtagaac
    23341 atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataatcgc gggcagcgtg
    23401 acgatacgaa accgttgcca ggcattagcg ccatccatct ctgccgcttc gtacagcgat
    23461 ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa gtagcgccag
    23521 atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcgaacca cagcggtgcc
    23581 tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta gccgttgttg
    23641 aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga gaggatcacc
    23701 agcgaacgcg cggttttgcg cagacggaac tcgcggttga aaaacatcgc cactgccagc
    23761 ccgagcacgg tgctgcccgc caccaccagc gcggtatacc agaccgtcat ccacagcgag
    23821 tgccagaagc cgggatcgga gaggatacgc acatagttac tcaccccaac aaaggttgac
    23881 tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga caccattggc
    23941 caggccacca gaccacccag cagtaataag ctgggcgcga gaagcagcag cgcaaagggc
    24001 atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg gtttagtgct
    24061 gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg agatcggctt
    24121 tacccacggt gacgttatgc accatggagc tcaccacgcc agaccccgtc acatcaccca
    24181 tgcgggtaaa gtttttatcc cctactgcac caaagacctg aatatttggc agttcaccga
    24241 ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc caggtggcgg
    24301 tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc acccagtcgg
    24361 caatgttgtc tgcctgctcc ataaaggtga caaatttttc tgctgcttcc gtctcttcgg
    24421 tcttttgccc ggcggtaatg gtcagcgagg tcaacatgcc gtagaccgca gagtttttct
    24481 cggttggcac cacgaaaccg acgtttttcg ggtcgccttc tttaatcaca gccggaagga
    24541 tataggtgga gtaaatcgcc atcggcgcgg tgccgttcat aaaggcgtct ttcacttcca
    24601 tgatgtcgtt agaacccggc atagtgttgg cagcaaggtc gcggtaatag gtcagtgcct
    24661 gcatcatctc tggtgtatca agggtgattt tgccttcggc gttaaagacg ttagcctggt
    24721 tggataacgc aaactgggag aaggattgtt ccgtcaacac gctttctgct gtaggcagcg
    24781 caatgccgta ttttttattc gccgggtcat tcagtttctg tgcaacgtcc agcagctgtt
    24841 gccagttttt cggctcctca agccccgctt ttgccagcac atctttgcga taccagatac
    24901 cgccaatcca ggcgctgaca ggaacaccgg tccatgcgct accatcttcg gtacgcacaa
    24961 tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt gctgatgact gtggcaaccg
    25021 ctttgcgatc gataagctgc tctttgtcca tcactttggc gtagtcatgg ctggtttcta
    25081 tcacttccgg cagcgaaccg ctacgtgaaa gagtaatgac tttagtgtta taggcatctt
    25141 cttccacggg cacttgctta acggtgatgc cagggttttc tttttcaaaa cgggcaatca
    25201 atttactgat aacggcctgg cgctcctgct ccaccgaaga atgcataaat tcgatggata
    25261 cattcgtttt attttcttct ttacaccctg aaatcagggc gcaggaaacc agtgctgata
    25321 acagcacgat ttttgattta atcatgagat gttccttttt atttaatcca cataacctgc
    25381 caggggcgca gcgttatttc attacccaat atatcaactt cactaatcag atcccgacca
    25441 tgcagattag taatatttac atgctgaata tttttactga cattaaacaa tccagtcaga
    25501 caattaccat cagcgttact tctttgaata cgcattacgg atgaattaat cgtatcgatg
    25561 gtaaaattat tatccggatg aaactcgtta tggctgcgac gaagtgtaat taaacgcgac
    25621 aactcatgat ataccgcatg ccttaatgta gcttcatcgt tcagttctcg ggttatctct
    25681 ttactgtgat attttttacg gttaatcgca cggttatatc cgagtttttc gacacctgcg
    25741 taatcattac gcgagccaag aatactttga atatatatcg ctggcacacc ggggaaactt
    25801 aacaaaatcg catgggcgag aataaacctg gcgcaacgtt cttcatcgct actctcacgg
    25861 cggcttaacg catccatata ggtcacattt atttcatacg gactgcgtgt accgtcggga
    25921 ttatttttcc agtttactaa tgcaccttcc tgctgtaacg cctcgaccag ctctaatatt
    25981 tcgctttcag gcaacaagcc ccgtagcggg tttagcccga tgccatcgtg agaggcgagg
    26041 aagttaaacc aggtggtgtt gctggaaggt agtgtcaggt tttgcgccca cgcacaaagc
    26101 gcctcaacgt tctgtttttg caccgcatgc agcaccagtg gcggcagcga gaactggtac
    26161 accatatgtg cttcgtcatc gcctgcgcca aagtaagcaa tgttgtcttt atgcggaaca
    26221 ttggtctcgg taatgatcac tgtacctggc gcaacgttat caataatcga ccgtaacagt
    26281 ttgataatca gatgtgtttt ttccagatgg atgcagcttg ttcccggctc tttccacata
    26341 aagccaacgg catccaggcg gacatactca gcacctttcg caaggtaaca gagtaaaaca
    26401 tccaccatcg ccagcaacac ttcagggcta cggtaattca ggtcaatttg atcgtcacta
    26461 aaggtggtcc ataaatggcg cgttgaatga tcgcgcatct ggaatggcgt taataacggt
    26521 aacgcacgcg ggcgagtgac ggcgctgaga tcggtttgcg ggtcaacggc aataaaaaaa
    26581 tcttcaaaac ctggatgctg ttgtaaatag tttttaaatc attcactttt tgccgacata
    26641 tggttgcaga caaaatcaaa cattaaatga ctgcattcac cgagttgctg aatatcctgc
    26701 cactcccccg cttcactggc gacctgatga taatcaatta ccgaaaagcc atcatcagat
    26761 gaccatggat aaaacggcaa caaatgaaca tgtgaaaaaa tgctttgcag ccattgatgg
    26821 taaaactgat taaatgtggg taatggtttt aaatcattgc tgtgaaattg atcggcatag
    26881 gtaattaaca cgacatcact ttcatcccag tgttttttac gtcgctgtgt aattaatcgt
    26941 ttcgcactct caagacgcgt tacaagtttc tgtaaatgag ttgcggtaaa tgttccaccg
    27001 tagatttcgt ccaggtaatc cgtaattttc tgtttcatgt tgcctttccg tggtagcggt
    27061 cccatgaaac gagagactac tccgcagaaa ataaactgtc aattgagcac aagggtttta
    27121 gtcacgcagg aaatcatttt ttaccaggga aaaagcgtat ttgtgagctg acgcaaataa
    27181 tctatgggga tgtaaataag gtgtgacaga aaaaaaacgg cgcataagcg ccgctcatgg
    27241 tgaattcttt taacctttga ccttcggcat tgcgatgtct ttcaggccac cggcgttctc
    27301 aacgtgggta tatcccatct cgctaaggat ctcttttgct tgccctgact ggcgtccggc
    27361 attgcaatac actttcacgg tgtcgttttt atccggaacg gcggtggcaa tgcgctcttt
    27421 cacttccttc aggggaatat tgatggcccc ctgaacgtgc tcttgctgat actgctctgg
    27481 aacacgaaca tcgatccagt gttcagcggc gaaaacgggc agtgaaaaca ccagtgccag
    27541 agcaagtaag ccttttttaa acatgtaagc gtccttctat ggatgaaaat tgccatccat
    27601 tatacgcctg agatttaaca atttttgcga aactcccctc accttttata acgctgtgcc
    27661 agtttattag cagcccgact taacagcggt tccagcgcca cagccagcaa cattttcagc
    27721 ggtcggcgag caactgattt tatcgcccag cccgctacac ccgccgggcc atagcgcagt
    27781 gcggtaagaa gtaccagctt gcctgctaat ttgaaaccag gctttacctt ttgcccggcc
    27841 tgttgccagc gagtattcat aactttcctc acagttgacg gaaacggcta cgtaacgtga
    27901 aagtatcgga agtgacataa cgttccatct cgcgtaaacg cgtttcactt gccgccagtt
    27961 cacggtcgac ttcatccagc aattcgctgc tggaaggtag ctgctcacca aaggccatgt
    28021 tgtccggcat tggatcaagc gcaaatgaca aaatgatgta agcaaccagg gtaaacagcg
    28081 ccagaccgaa gaaaatcgac agcaccacca ggatacgcac cagttttgcc ggtacatcaa
    28141 aatagttggc aatcccggcg cagacgccgc ggaccatgcc ctgctgtgga atacgccata
    28201 attttttatt gagattaatg cccgccatta gcgatccctc cagttcggat gttctgcatc
    28261 aagaatagat tccagcgcct gaatacgttc gcgcatccgt tttgcttcat cagccagttg
    28321 cgctaatcgc tgctgctcac tttgcgacaa ttcactgcga ccagaacgat tgctgtaatg
    28381 cagccataac cagatcggta aaacaaacag cacaaaaatg gttaacggaa tagccagaaa
    28441 tagcgcgctc ataagtactc cttagacgat gagcggcggc gcgttcggac gccgcctgga
    28501 tgttattatt gattgtcttg cttcattttg gcttttaatt gtgccagttg ttcgctgatt
    28561 gcatcatcgg ctttcagttc ggcaaactga tcgtccagcg atttttgttt accgaagctg
    28621 tggctttctg cttccgcttc catctggtca atacgacgtt cgaaagattc gaaacgagcc
    28681 attgcttcat ccagtttgcc actgtccagc tgacgacgca catcgcgcga cgagtttgcc
    28741 gcctgatgac gtaacatcaa tgcctgctgg cgagcgcgtg tttcgctcaa tttgttttcc
    28801 agctcaccga tctctttctt catgcgtgcc agcgtgtcgt ccaccagcgt cacttcatgt
    28861 tccaggctct taatcagatc ggtcagtttc tgtttttcaa ttaacgctgc acgtgccaga
    28921 tcctctctct ctttcagcag cgccagttcg gctttttcct gccattcaac ttcacgcgcc
    28981 gacgcttgtt caatacggcg agtcagctgt ttcttttctg ccaacgcgcg cgccgaagta
    29041 gaacgtactt caaccagtgt atcttccatc tcctggatca tcagacgaac cagtttctgt
    29101 ggatcttccg ctttctctaa cagagcgttg atgttggcat tcacgatgtc ggcaaagcga
    29161 gaaaaaatac ccataatgtt gtcctcttga tttctgcgtt caggattgtc ctgctgaact
    29221 gttaatacaa tttgcgtgcc aattttttat ctttttgatt tataaagatc tgattgaaga
    29281 accaacagca acatgccagg atgagttagc gaattacact aacaagtggc gaatttcatc
    29341 atggcagaat acaaagataa tttacttggt gaggcgaaca gctttctcga agtgctggaa
    29401 caggtttcgc atctcgcacc gctggacaaa ccggtgctca tcatcggcga acgcggcacc
    29461 ggtaaagagc tgattgccag ccgcctgcat tatctctcct cccgttggca agggccgttt
    29521 atttccctta actgcgcggc gttaaatgaa aatctgctgg attccgaact gtttggtcac
    29581 gaagcggggg cgtttaccgg tgcgcaaaaa cgtcatcctg ggagatttga acgtgccgac
    29641 ggcggtacgc tatttcttga tgaactcgct acggcaccga tgatggtgca ggagaaatta
    29701 ttgcgcgtga ttgagtacgg tgaactggag cgcgttggcg gcagccagcc attgcaggtg
    29761 aatgtgcggt tggtatgcgc gacgaatgcc gatctcccgg cgatggtcaa tgaaggcact
    29821 tttcgcgctg acctgctcga ccgactggct tttgatgttg tacaactgcc accactgcgc
    29881 gagcgcgaaa gcgacataat gttgatggca gaacactttg ccatccagat gtgtcgggaa
    29941 attaagctgc ctctgttccc ggggtttaca gagcgcgcca gagaaacatt gctgaattat
    30001 cgttggccgg gaaatattcg tgaattgaaa aacgtggtgg aacgttcagt gtatcgccac
    30061 ggcaccagcg attatccgct tgatgacatc attattgacc cctttaaacg gcgtccgcct
    30121 gaagacgcta tcgccgtttc agaaaccacc tcgcttccaa cactgccgct ggatttacgt
    30181 gagtttcaga tgcagcagga aaaagagttg ctgcaactca gtttgcaaca agggaaatat
    30241 aaccagaagc gtgcggctga attactgggg ttaacttatc atcagttccg cgcgttgttg
    30301 aaaaagcacc agatttagcg aagttgagca atacaacttg ccggatgcgg cgtgaatgct
    30361 ttattcggca tacataatca atacgtcgtt tgcaggcctg ataagcgcag cgcatcagac
    30421 attattgcgt tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg
    30481 aattgcgcat ccggtatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca
    30541 caggtcagca ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc
    30601 gctgacggct cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg
    30661 cgtaccgcag caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc
    30721 tggcccagtt gattcgcgcg ttcgcagagt gattctttgt cgataatatt gagcaccgcg
    30781 tgcgcggcag ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg
    30841 ggcgcgtcca taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg
    30901 cttttcgcca tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta
    30961 ccggtacgcg caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg
    31021 cacaggcggc gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc
    31081 tgcaccggtt cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag
    31141 cgttcgatgg cgtcgaggga gtcctgtgtt gaaatgccgt gtaaatctga cggataaggt
    31201 acgtgataca ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg
    31261 gtcagcgcca tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca
    31321 ggtcgtccgg tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta
    31381 gtgaagaatg cggttttagc ctgcccgctc actggggcaa gggcgttaat tttctccgcc
    31441 agcgtgacgt agctttcgta cggcacaatc tgatacgccg tgtgggtaaa ctgttgcaat
    31501 tgctgctcca ccgccgcgac cagatcaggg tggcgatgcc cggtattcag caccgcaatg
    31561 cctgcggcga aatcgatgta ttcgttgccc tctacgtcct tcagcgtagc gttttctgcc
    31621 gactgggcga agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc
    31681 tgatggaatt cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa
    31741 ttaaacgccc agacgatcgc gcaggctgta ataagccgcg cccatcgcgg taaatggcac
    31801 gcgcagcgtg cgcccgccgg ggaacgggta atgcggcaga ttggcgaagg catcgaaacg
    31861 ttcggcgtcg ccgcgcagca gttcggcaat cagacgcccg gcaagatggg tacaggtgac
    31921 gccgtggccg ctgtagccct gcatgtaata gatgttggta tcaaggcgac cgaactgcgg
    31981 catgcgcgac aaggtcagca ggaagttgcc cgtccagcgg taatcaattt tcacgccctt
    32041 cagctgcggg aaggttttca gcagtttcgg caccacaagg cgctcaacgt catccgggtc
    32101 gcgcgcgccg tagaccacgc cgccgccgta cagcaggcgg ttgtcggcgg taagacggta
    32161 gtaatcaagc agatagttac agtcttccac acagtagttt ttcgggatca gcgaacgggc
    32221 taaatcttcc gacagccgtt cggtggtgat cacctgcgtg ccgcacggca tgctgcgttt
    32281 cgccagttcc ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt
    32341 cgccgtcacc tgacctttgg cagttcgcac aacggctggc gtggtgtgct ggatctgcgt
    32401 cacggcagaa agttcataca cgcgcccgcc gttgaggcgg atggcgtccg cttcgccaat
    32461 ggcgaggttt agcggatgaa tatgcccacc gctgtgatcc agcagcgcac cggtatagcg
    32521 atcgctggct acttcacggc gaatggcgtt ggcgtccagc aattccagct gtttattgcc
    32581 gtagcgttcc cagttctctt tctgctcttc aagtgtggcg agctgtttat cattcatcgc
    32641 cacaaacagg ccgccggggc ggtagtcgca gtcaatctga taacgtttga tacgttcgcg
    32701 gatgatctca ccaccttcga acatcatgct gccgagcata cgggcggtgt ccatgccgta
    32761 gcttttttcg atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg
    32821 cccgcttgcg ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc
    32881 cgccagatgc agcgcggaag agagcccggt atagccgccg ccgaccacgc aaacgtcgca
    32941 ggaaatcgac tcattcagcg tgtcgaatgg cgcgtattta ttcgcactgg cggcgtagta
    33001 actgctggta tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcgg
    33061 tgaatttttc aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc
    33121 caaacggcac ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccagccttca
    33181 ggcggcggct catgcggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc
    33241 cgtactggct gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca
    33301 ccagcaccgg accgaaaatc tcttcgcggc ttaaggacgc attcgggtct acatccacaa
    33361 agatggtcgg gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc
    33421 ctttgctttc gccttcccga ataaagctat ggaccgagtc agcgtgagcg ctgtcgatta
    33481 aggtgcccat ggtggttgcg ggatcaagtg gatggcccgg ctgccagttt tgcgcctgct
    33541 gttttaacag ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg
    33601 cgatgcacac ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt
    33661 gcagatccgg acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga
    33721 cgcgtttcat gttgctctcg cccgcatctt tcagcagctg tttcccggta cgggtcgaac
    33781 cggtaaaggc aatggcgtcg atatcgttat gacgcgacag cgcctgcccc gcttcatgac
    33841 caaaacctgt caccacgttc aaaacaccat ccggcaagcc tgcttctttc gccagccccg
    33901 cgagacgaat cgcactgagc ggtgattttt cagacggttt caggacaacg ctgttccctg
    33961 ctgccagcgc cgggccaagc ttccagcagg tcagcagcag cggaaagttc cacggtacga
    34021 tggcggcaat cacaccgacc ggttcacgca cgatcatcgc cagctcatgg ctactggtgg
    34081 tcgccacttc gccatacact ttgtcgatcg cttcggcgta ccagcgaatg gcgcgcgccg
    34141 cgccgggaat atcatcacgc agactgtgac gaatcggttt gccggtgtcg agagtttcca
    34201 gcagtgccag ctcttcggcg tgggcttcca ttaaatcggt gagtttattc agtaccgctt
    34261 tacgtttagc cggagaagag agtgaccagt cgccgcgttc aaatacgccg cgtgctgcgc
    34321 tcaccgcacg gtcgatatcg acgctcttgc cgcgggcaat tttcgccagc ggtgcctggg
    34381 tgaccggatc aacggtttca aaggtttcat tttccgccgc agcagtatat tcaccgttaa
    34441 taaataagcg ggtttcaatg gcgagactta acgctttatc ctgccagtaa gccagatgat
    34501 gaaaattcat tatgactcct gtttcacgtc tatcagatat atgcagtttg tgaacgtcag
    34561 ggcgcagaga tccccgggcc gtatgcccgt ttatttctgg cgacattctt tatatacact
    34621 tcatccttca agccgcctct gccttggctg cgttcgctcc cccgaatcac ttacctgagt
    34681 aaactcatcg ggattcgctc acttgccgcc ttgatgcaac ttgaatgatt ttgtgtatag
    34741 attgccgggc gcattgcgct tacccggctt acagaacaaa aagattaaaa cgtggtgggc
    34801 gtatgggcgc tgataattcg gcaaatacct gccgacgtat tactgaaact gtgcgggatg
    34861 ccggtattaa tggcataact ttgccccgcg acgaggtggt aatcctgacc attaatcgtc
    34921 agaacaattt caccttccag tactgtgcct atttcctcac cctgatgctt aattctttcc
    34981 ccagtggttg tgcccggctg gtacgtttca aagatcatcg ccagcgtgcg attcgggtta
    35041 ccgttatgaa ccagcttcat tgacacaccc tgactgccca tctcaattaa gtcgtcctga
    35101 ttaatgacga cctgcggctc atcaggtttt tccggctcgg aaaagaattc cgagagtgac
    35161 agaccataca ccttcagcag cttttgcagc gtactgatgg cagggctgac tttatcttgt
    35221 tctatcgtac tgatagcact gtgagtcagc ccggagagtt cggcggcacg acgttgtgaa
    35281 agcccctgct gctggcggat ttccgacaag cgttttcctg gcgccagtcc ctcatcactc
    35341 atatttgcat ttccttaaac tgtagtggtc agagtcgctg tttttcagcg atatggtgct
    35401 gacaagcggt gataaagccc tcgaacaata tacgcgaaag cgcgtactcg ctactgttcc
    35461 attccgggtg ccactgtacg cccagcgcaa aaggatgatt gatgacgcta accgcctcaa
    35521 ctaagccatc cggcgagcga gcttcaacac gcaaccgtgg gctaacgacc ttcgcccctt
    35581 gcccatgcag agagtttacc caaaagttgc tacattcagg taacaacgca gacagtaatc
    35641 ccccctcttc aacctgaact tcatgcgacg gtgcatattg ctgttccacc ggcagttcgg
    35701 gatcttcccg atgttccagc aattcaggct gttcgcacag cttgcgatgc aacgaaccac
    35761 cggttgccac caccagttcc tgtaaacccc ggcagatggc gaaaatgggg atgcgccttt
    35821 cgagtgcggc attaattaac gccatgctca gaagatcacg cccgggatcg gcgtcaggct
    35881 catcgccgtt ttcaccatat aggtgcggct gcacattgct gggactacca ggaagataaa
    35941 tgccatcgag tttcggcaaa agttgttcaa gtaatgacgg ttccgccagc gcatgtggta
    36001 gcgcaattgg caagccgcct gcgtggatga tggcattcag gtacttttct tgcagagtct
    36061 gggtcgcatg acccttaagc ctgttcctgc acattacgac accgataacc ggattgttca
    36121 ttatattttc catgctcaat ctcacaaagt ggactaaatt atcgccatta ctgcggttta
    36181 aatgaccttt atgttcaata ttttttcaat ctagcagtgg gttttcattt ttgcaaactc
    36241 aatttaacat ttgacaaaca tttagtttgc atacagattc gaatggtggt cattatattt
    36301 tacgctttga taacgagcgg aaaacaaacc aaaggcgaag aatcatggaa accaatatcg
    36361 ttgaagtaga gaactttgtt cagcagtcag aagagaggcg gggtagcgcc tttacgcagg
    36421 aagtgaaacg ctacctggag cgctacccga atacgcaata tgttgatgtt ttacttactg
    36481 atttgaatgg ttgcttccgt ggaaaacgta tccctgtttc aagcctgaag aagttggaga
    36541 aagggtgtta tttcccggcc tcggtgtttg caatggatat tctgggcaac gtagtagaag
    36601 aagcgggtct gggtcaggaa atgggcgagc cggatcgtac ttgtgttcct gttctcggtt
    36661 ccttaacccc ttctgccgcc gatccagagt ttatcggtca gatgctcctg accatggtcg
    36721 atgaagatgg cgctcccttt gacgttgagc cgcggaacgt tcttaaccgc ctgtggcagc
    36781 agctacgcca gcgcggattg ttcccggttg tagcggtaga gctggagttc tatttactgg
    36841 atcgtcagcg cgacgctgaa gggtatctgc aaccgccctg cgcgcccggc accgatgacc
    36901 gcaatacaca aagccaggtt tactccgttg ataacctcaa ccacttcgcc gatgtgctca
    36961 atgatattga tgaactggcg cagttacagt taattcccgc cgacggtgcg gtcgccgaag
    37021 cctcgccggg tcagtttgaa atcaaccttt accataccga taacgtgctg gaagcctgcg
    37081 atgatgcgct ggcgctaaaa cgcctggtgc gtctgatggc agaaaagcat aagatgcacg
    37141 ccacttttat ggcgaagccg tatgaagagc acgcgggcag cggaatgcat atccatatca
    37201 gtatgcaaaa taaccgtggc gagaacgtgc tttctgacgc ggaaggcgaa gattcgccgc
    37261 tgctgaaaaa gatgctcgcc gggatgattg acctgatgcc gtcgtcgatg gcgttgctgg
    37321 caccaaacgt gaactcgtat cgccgcttcc agccgggaat gtatgtgccg acgcaggcgt
    37381 cgtggggcca taacaaccgc accgtcgccc tgcgtattcc gtgcggcgac cgtcataatc
    37441 accgcgtgga atatcgcgtg gcgggtgccg atgccaaccc atatctggtg atggcagcga
    37501 tttttgccgg tattttgcat ggccttgata acgagctgcc gttgcaggaa gaagtcgaag
    37561 gcaacgggct ggaacaggaa ggcttaccct tcccgattcg ccagagcgat gccctgggtg
    37621 agtttatcga gaatgatcac ctgcgccgct atttaggcga acggttttgc catgtgtatc
    37681 acgcctgtaa aaatgatgag ctgttgcagt ttgagcgcct catcacagaa acagaaattg
    37741 agtggatgtt gaaaaacgcg taattcccca tgcggggttg tttcatcggc ctgagcggag
    37801 ccaggcctga catttgctgg taatccagac atttcccgga tgtcgcgtta tggagcgcag
    37861 gcggcaacgg gcttccgtta acgattaaga ctgtgacgag gaaatgagat gatgccgatg
    37921 attaaatccc cgcatggcga gggaggctgc gtatgcgccc ctcctgctac tgactggact
    37981 ccgccccctt tgcttccctt gctaaacagg tttgatttca ggtcgacacg accgcaaacc
    38041 ttattacgca ggggaggcag caattatggc tattaattca ccactgaata ttgctgcgca
    38101 acccggcaaa acccgtctgc gaaaatcact gaaattgtgg caggtggtaa tgatgggtct
    38161 ggcctatctc acgccgatga ccgtatttga tacttttggt attgtctccg gcattagcga
    38221 cggtcacgtt ccagcgtcct atttgctggc gctggcgggc gtgctgttta ccgctatcag
    38281 ctacggcaaa ctggttcgcc agtttccgga ggccggttcg gcctatacct acgcgcaaaa
    38341 gtcgattaat ccgcacgtcg gatttatggt cggctggtca tcactgctgg attatctctt
    38401 tttgccaatg atcaacgttt tgttggcgaa aatctatctc tccgccctct tcccggaagt
    38461 gccgccgtgg gtgtgggtgg taaccttcgt cgccatttta accgccgcga atctgaagag
    38521 cgtcaacctg gtcgctaact tcaataccct gtttgtactg gtgcaaatct ccatcatggt
    38581 ggtgtttatc ttcctggtgg ttcagggact gcataaagga gaaggcgttg gcaccgtctg
    38641 gtcacttcag ccgtttatca gcgagaacgc gcacctgatc ccgattatta ccggtgcgac
    38701 gattgtctgt ttctcgttcc tcggtttcga tgcggtgacc acgctttcgg aagagacgcc
    38761 ggacgccgca cgcgtgatcc cgaaagccat cttcctgacg gcggtatacg gcggcgttat
    38821 ctttatcgcg gcgtcgttct ttatgcagct gttctttccc gatatcagcc gctttaaaga
    38881 cccggacgcc gcactgcctg aaattgcgct gttcgtcggc ggcaagctct ttcagtcgat
    38941 tttcctctgc accacgtttg tgaacacatt agcgtctggc ctggcttcac atgccagcgt
    39001 gtcgcgtctg ctgtatgtga tggggcgtga caatgtgttt ccggagcgcg tgtttggcta
    39061 tgtgcaccca aaatggcgga ctccggcact caacgtcatt atggtcggga ttgtcgcgct
    39121 gtcggcgctg ttctttgatt tagtcaccgc gacagcactg attaacttcg gtgctctggt
    39181 ggcgtttacc ttcgtgaatc tgtcggtgtt taatcatttc tggcggcgta aaggaatgaa
    39241 taaaagctgg aaggatcact tccactattt gctgatgccg ctggttggcg cgctgacggt
    39301 gggtgtgctg tgggttaacc tagagtcaac gtcactgaca ctcggtctgg tatgggcttc
    39361 gttaggcggc gcatatttgt ggtatctgat ccgccgctat cgcaaagtgc cgctgtacga
    39421 aggtgacaga acgccggtga gcgaaacgta atagccggat gcgcggcgca ttatcctgcc
    39481 tgagggtaac gcgccgcgcc attaactata aaaagatgta tgacccggat cacgagtgct
    39541 atcatccgcc cttatgctta gcgcattaac cccaggggca aataatgaac cacgatatcc
    39601 cgctaaaata ttttgatatt gccgatgagt acgcgaccga atgcgcagag cctgtcgcag
    39661 aggctgaacg cacaccgctg gcccactact tccagttact gctcacccgt ttgatgaaca
    39721 atgaagagat cagcgaagaa gcccaacatg aaatggctgc tgaagcgggg attaatcccg
    39781 tacgcattga tgagatcgca gagtttttga atcaatgggg taatgagtaa ttatttaatt
    39841 gcagacgatc ggtcagtatc gttgtccgga taaagcgttc acgccgcatc cggctaacaa
    39901 tgacacgcac tgcagcgtgc tttaatgggt ttctcacgat cgtaccgctc tgctcctcgc
    39961 taagttgttc tgtgagttat agttatagag aaatcacggg tacgaatgtt gaacagatgg
    40021 cgacgccacc tgttcaacga ccaaccacac ggccctcatt acgacccctg aacacaaacg
    40081 gtaatggcaa atgtcattaa atattagtaa gatagcctct ttacataaaa tcccttaata
    40141 ttatgcgcca ggtattatcg tctcttttgg tgattgctgg acttgtgagt ggtcaggcaa
    40201 tcgccgcgcc tgaatccccc ccgcatgctg atatccgcga cagcggtttt gtctattgcg
    40261 tcagcgggca agtcaacacc tttaacccat ccaaagcgag cagtgggtta attgtcgata
    40321 cccttgccgc ccagttttat gatcgactgc tggatgtcga tccctatacc tatcgcctga
    40381 tgccggaact tgccgaaagc tgggaagtac tcgacaacgg cgcgacctat cgcttccacc
    40441 tgcgtcgcga tgttccgttt caaaaaaccg actggtttac tcccactcgt aaaatgaatg
    40501 ccgacgatgt ggtgtttacc ttccagcgaa tttttgaccg caacaacccg tggcataacg
    40561 tcaacggcag caacttcccc tacttcgaca gcctgcaatt tgccgataac gtgaaaagcg
    40621 tccgcaaact ggataatcat accgttgagt tccgactcgc ccagccggat gcttcttttt
    40681 tgtggcacct cgcaacccat tatgcttcgg tcatgtcggc agaatatgcc cggaagttag
    40741 agaaagaaga tcgccaggag caactcgacc gtcaaccggt cggcaccggt ccgtatcagt
    40801 tgtcggaata ccgcgccggg caatttattc gcctacaacg tcatgatgac ttctggcgcg
    40861 gtaaaccgtt aatgccgcag gtagtggtgg atttaggctc cggcggcacc ggacgtctgt
    40921 cgaaactcct gaccggggaa tgcgacgttc tggcctggcc tgctgccagc cagctatcca
    40981 ttttgcgtga cgacccgcgc ttgcgtttaa cgctgcgtcc tgggatgaac gtcgcctatc
    41041 tggcatttaa caccgccaaa ccgccgctaa ataatcccgc tgtccgccat gcactggcac
    41101 tggcgattaa taaccagcgc ctgatgcaat ccatctatta tggtacggct gaaacggcgg
    41161 cctctatttt accgcgcgcc tcgtgggcct atgacaacga ggctaaaatt actgaataca
    41221 atccggcgaa atcgcgcgaa cagttgaagg cgttggggct ggaaaactta acgctgaaac
    41281 tgtgggtgcc cacacgttcg caggcgtgga accccagtcc actgaaaact gccgaactga
    41341 ttcaggcgga tatggcgcag gttggcgtaa aagtggtgat tgtgccggta gaaggtcgct
    41401 ttcaggaggc gcggttgatg gatatgagcc atgatctgac gttatccggt tgggcgacgg
    41461 acagtaacga cccggacagt ttcttccgtc ctttactgag ctgcgcggca attcattcac
    41521 agaccaacct cgcccactgg tgcgatccga aattcgacag cgtgttgcgt aaggcgctct
    41581 cctcgcagca gctggcggcg cgtattgaag cctatgacga agcgcagagt attctggcgc
    41641 aggaattgcc cattttgccg ctggcgtcgt cattgcgttt gcaggcctat cggtacgata
    41701 tcaaaggtct ggtacttagc ccgtttggta acgcctcctt tgctggggtg tatcgcgaga
    41761 aacaggatga ggtgaaaaaa ccatgattat cttcacctta cgccgcattt tgttattgat
    41821 tgtcaccttg ttcctgctga ccttcgttgg cttcagcttg agctatttca cccctcatgc
    41881 gccgttacaa ggcgcgtcac tgtggaatgc ctgggtgttc tggtttaacg gcctgatcca
    41941 ctgggatttt ggtgtttcca gcattaacgg tcaaccgatt gccgaacagt taaaagaggt
    42001 cttcccggcg acgatggaac tgtgcatcct cgccttcggt tttgcgctga ttgttggcat
    42061 ccccgtgggg atgattgccg ggattacgcg ccataagtgg caggataact tgatcaacgc
    42121 catcgcctta ctgggctttt caatcccggt gttctggctg gcgcttctgt tgacgctgtt
    42181 ttgttcactc acgctaggtt ggttgcccgt ttcagggcgt ttcgatctgc tctacgaagt
    42241 gaaaccgatt accggttttg cgttgattga tgcctggctt tcggactcac catggcggga
    42301 tgaaatgatc atgagcgcaa tccgccatat gatattgccc gtgatcaccc tgtcggttgc
    42361 gccaacaact gaagtgatcc gcctgatgcg tatcagcact atcgaagtgt atgaccagaa
    42421 ctatgtaaaa gcggcggcga cccgcggttt gtcgcgcttt actattttgc gtcgccacgt
    42481 tctgcataac gcgttaccgc cggttattcc tcgcctgggc ttacagtttt ccaccatgct
    42541 gacgctggcg atgatcaccg aaatggtttt tagctggccg ggtttagggc gctggttaat
    42601 taacgccatc cgccagcagg attacgcagc catttccgcc ggagtgatgg tgtgtggctc
    42661 actggtcatt attgttaacg tgatttctga tattttgggt gccatggcta accctctgaa
    42721 acataaggaa tggtatgcct tacgatagcg tatacagcga aaagcgcccg ccgggcacgc
    42781 tgcgtaccgc ctggcgcaaa ttttatagtg acgcctctgc gatggtcggc ctgtacggct
    42841 gcgcggggct ggctgtactg tgtatttttg gcggctggtt tgcgccttac ggcatcgacc
    42901 agcaatttct cggttatcaa ttattgccgc cgtcatggtc gcgctatggc gaagtttctt
    42961 tcttcctggg gactgacgac ctcggacgcg atgttttaag ccgtttactg agcggagctg
    43021 cgccaaccgt gggtggcgca tttgtggtga cgcttgccgc gacgatctgt ggcctggtac
    43081 tgggcacctt cgccggggcg acacacggcc tccgctcagc ggtgcttaac catattctgg
    43141 ataccttact ggcgatccct tcgctgctgt tggcaattat cgttgtagcg tttgccggac
    43201 cgagtttgtc ccacgctatg tttgctgtct ggctggcatt gctgccgcgt atggtacgtt
    43261 cgatttacag catggtgcat gacgaactgg aaaaagagta cgttatcgcc gcccgtctgg
    43321 atggcgcatc aacgctgaat attctctggt ttgctgtgat gccaaacatc accgccgggc
    43381 tggtcaccga gatcacccgc gcactgtcga tggcaattct cgatatcgcc gcgctgggct
    43441 ttctcgatct cggcgcacaa ctcccctcgc ctgaatgggg agcgatgctc ggtgatgcgc
    43501 tggaactgat ttatgtcgcc ccgtggactg tcatgctgcc aggtgcggca attatgatta
    43561 gcgtgttgtt ggttaacctc cttggcgatg gcgttcgtcg tgcgattatt gcgggggtgg
    43621 aataatgcca ttactcgata ttcgtaacct gaccattgaa tttaaaaccg gtgatgagtg
    43681 ggttaaagcc gtcgaccggg taagtatgac cttaaccgaa ggtgaaatcc gcggtcttgt
    43741 tggtgaatcc ggttccggca agagtttgat tgcgaaagcg atttgtgggg tgaataaaga
    43801 taactggcgt gttactgctg accgtatgcg ttttgatgat atcgatttgc tgcgtctctc
    43861 cgcacgcgaa cggcgcaaac tggttggcca taacgtgtcg atgattttcc aggaaccgca
    43921 gtcgtgtctt gacccttcag aacgtgtggg tcgccagttg atgcaaaaca tcccagcctg
    43981 gacctataaa ggccgttggt ggcagcgttt tggctggcgc aaacgccgtg ccattgaact
    44041 gctgcaccgc gtggggatta aagatcacaa agatgcgatg cgcagttttc cctatgagtt
    44101 gaccgaaggt gaatgtcaga aagtgatgat agccattgca ctggcgaatc aaccgcgtct
    44161 gctgattgct gacgaaccga ccaactcaat ggagccaaca acccaggcgc aaatctttcg
    44221 cctgctgacg cgtctcaacc aaaacagcaa taccactatt ttgcttatca gccatgactt
    44281 acaaatgctt agccaatggg cggataaaat taacgtgctt tactgcggtc aaacagtgga
    44341 aaccgcgccg agtaaggagt tggtgacgat gccacatcat ccttataccc aggcgctgat
    44401 ccgcgcgata ccagacttcg gcagcgcgat gccgcataaa agtcgcctca atacgctgcc
    44461 cggcgctatc ccactgctgg aacagttacc gattggttgt cgtctggggc cacgttgccc
    44521 gtatgcacaa cgagaatgca ttgtgacgcc acgtttgacg ggggcgaaaa atcatctcta
    44581 tgcctgtcat ttcccgctga acatggagaa agagtgagat gatcgaaacg ctgcttgaag
    44641 tgcgtaattt aagtaaaacg ttccgctacc ggaccggctg gtttcgtcgt cagaccgtag
    44701 aagcggtaaa acccttgagc tttacgctac gtgaaggcca gacactggcg attattggcg
    44761 agaatggttc gggtaaatcc acgctggcaa aaatgctggc gggaatgata gagcctacca
    44821 gcggcgagtt attgattgac gatcatccac tgcattttgg cgattattcc ttccgcagtc
    44881 agcgtattcg catgattttt caggatcctt cgacctcgtt gaatccccgt caacgtatct
    44941 cgcaaattct ggattttcca ctgcgcctga acaccgacct ggaaccggaa cagcgtcgca
    45001 aacagattat tgaaacgatg cgtatggttg ggctgctacc ggatcacgtc agttactatc
    45061 cgcatatgtt ggcacccgga caaaaacagc gtctgggtct ggcgcgcgcg ctgatattgc
    45121 gcccaaaagt cattattgcc gatgaagccc tcgcttcact ggatatgtcg atgcgttcgc
    45181 agttgattaa tctgatgctg gagttacagg aaaaacaggg catttcgtat atttatgtta
    45241 cccagcatat cggaatgatg aagcacatta gcgaccaggt gctggtgatg catcagggcg
    45301 aggttgtcga acgaggcagc accgcggatg tgctggcatc gccgctgcat gaactcacca
    45361 aacggctgat tgccgggcac tttggcgaag cattgacagc ggatgcgtgg cgtaaagatc
    45421 gctaattttc tgaatcctcc ttccagatta tccacctcat tctgacaaga gattaaatct
    45481 tgtcagaatg aggtgatgga taaaataaaa tcaaatgcgc gtgatttaag acgcaatctc
    45541 actttgcagg aacgaaagct ctggcgatat cttcgcagcc gacgttttgg tgatttcaaa
    45601 tttcgccgtc aacatccggt ggggagctac attctcgatt ttgcttgctg ctcggcgcgc
    45661 gtagtcgttg agctggatgg tgggcagcat gatttggcag ttgcctatga taccaggcgc
    45721 actagctggc ttgagtcgca gagctggacc gtgctgcgtt tctggcataa cgagattgat
    45781 tgtaatgagg aggcggtgct ggagattatt ctgcaggaac tgaaccgccg gtcaccctca
    45841 ccctgaaagg gcgagggggc cgaccgagcc gaatagctgt tgtggtgaaa acatggagac
    45901 agtgctggag aatattcggc aaggtctgaa ccatcccagc catcgccata aaagggttag
    45961 gggctgtatg agcctgtttg ttgctggggt aacaatattt gcacaatatg gtcccctcgc
    46021 ccctctgggg agagggttag ggtaagggga aaagcgccca ccctgccgca gcctgctccg
    46081 gtcggacctg gcaactatag ctactcacag ccaggttgat tataataacc gtttatctgt
    46141 tcgtactgtt tactaaaacg acgaatcgcc tgattttcag gcacaacaag catcaacaat
    46201 aaggattaaa gctatgggtt ttctttccgg taagcgcatt ctggtaaccg gtgttgccag
    46261 caaactatcc atcgcctacg gtatcgctca ggcgatgcac cgcgaaggag ctgaactggc
    46321 attcacctac cagaacgaca aactgaaagg ccgcgtagaa gaatttgccg ctcaattggg
    46381 ttctgacatc gttctgcagt gcgatgttgc agaagatgcc agcatcgaca ccatgttcgc
    46441 tgaactgggg aaagtttggc cgaaatttga cggtttcgta cactctattg gttttgcacc
    46501 tggcgatcag ctggatggtg actatgttaa cgccgttacc cgtgaaggct tcaaaattgc
    46561 ccacgacatc agctcctaca gcttcgttgc aatggcaaaa gcttgccgct ccatgctgaa
    46621 tccgggttct gccctgctga ccctttccta ccttggcgct gagcgcgcta tcccgaacta
    46681 caacgttatg ggtctggcaa aagcgtctct ggaagcgaac gtgcgctata tggcgaacgc
    46741 gatgggtccg gaaggtgtgc gtgttaacgc catctctgct ggtccgatcc gtactctggc
    46801 ggcctccggt atcaaagact tccgcaaaat gctggctcat tgcgaagccg ttaccccgat
    46861 tcgccgtacc gttactattg aagatgtggg taactccgcg gcattcctgt gctccgatct
    46921 ctctgccggt atctccggtg aagtggtcca cgttgacggc ggtttcagca tcgctgcaat
    46981 gaacgaactc gaattgaaat aatcattctg ttggtaaata ttgggcggcg ttctgccgcc
    47041 cgttatctct gttatacctt tctgatattt gttatcgccg atccgtcttt ctccccttcc
    47101 cgccttgcgt caggataacg atttccttta cgaccaagga gcgcccatgg aacaacgcca
    47161 catcaccggc aaaagccact ggtatcatga aacgcaatcc agtactgcgg agtatgacgt
    47221 tctgcctctg gtcccggaag ccgcaaaggt cagcgatccc tttctgctcg acgtgatcct
    47281 tgatgaagaa acgctggctc ccttcctttc atggctggtc cctgcgcgcg ttcttgcagt
    47341 ggaattgttc cctgaccagc ttaccgtgac ccgttcacag accttcaccg cttatgaacg
    47401 cttgtctacg gccctgacgg ttgctcaggt ttgcggcgtc cagcggttat gtaactacta
    47461 ttcggcgcga cttacgccgc tccccgggcc tgattcctcc agggaaagca atcataggtt
    47521 ggcacaaatc acgcaatatg cccgccaact ggctagctcg ccttctatta tcgacaaccg
    47581 atcgcgccag catctgaatg acgtcggtct tactgccggg gactgtgtga tcattaacca
    47641 aatcattggt tttattggct ttcaggcgcg gaccattgcg acatttcagg cttatctcgg
    47701 gcacccggta cgctggttac ccggtctgga aatacaaaac tacgccgacg cgtcactgtt
    47761 tgctgatgaa tcaatacgct ggcgaagcag ctatgaggtg gaaaaactac ctgaagagta
    47821 cacaaaaagt tcaacagcag aactttgcca actggccgaa acactctctc tccaccctat
    47881 ttcactttcc cttctcgaaa ggttgttaaa cagtacacgg gttaatacac agccggataa
    47941 taagcttgcg gcgttgttat gcgcgcggat aaatggcagt cctgcttgtt ttgccgcctg
    48001 tatggattca tcaaatgaat ataaaaaaat cagccccctt ctgcgcaagg gcgaaaatga
    48061 aattaaccaa tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg
    48121 gctgacccga gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga
    48181 aaaatcatca acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa
    48241 tcgcttaaaa atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt
    48301 aaaactgtca gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc
    48361 tgctagcgca gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa
    48421 aagccacaga aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc
    48481 cgccgccgca gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg
    48541 aaaaagaacg tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg
    48601 tgggtaaagt tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa
    48661 aagacgccat tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact
    48721 gggcggttgc cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac
    48781 tgacacaata catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc
    48841 gccataatct ggaaaaagaa gcaccagacg gcgtcgctac cgcaatgctc gatgaaggtc
    48901 tggttcgtga agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag
    48961 atatggatga cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg
    49021 cgattgccga tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa
    49081 ttcgcgcatt caccaactat ctgcctggct tcaacatccc tatgctgcct cgcgagcttt
    49141 ctgacgatct ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga
    49201 cgctctccgc tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat
    49261 ccaaagcaaa gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc
    49321 agcctgaaag tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc
    49381 gcggcgagtg gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta
    49441 ttctcggtga aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc
    49501 gtatcgtcga agaagcgatg attgccgcta acatttgtgc ggcccgcgta ctgcgcgata
    49561 agctcggttt tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc
    49621 tggcagcgtt gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg
    49681 acggtttctg caaactgcgt cgtgaactgg acgcgcaacc aactggtttc ctcgacagcc
    49741 gcattcgtcg cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc
    49801 tcggtctgga agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca
    49861 accaccgtct gctgaaagcg gttatcaaag gcgaaactgc gacgcgccca caggatgaaa
    49921 tcactgtcca aatggccgag cgtcgccgtc tcaaccggat ggcagaacgt gatgttggtg
    49981 actggttata cgcacgcttc ctgaaagaca aagccgggac cgacacccgt ttcgcagcgg
    50041 aaattgtcga tatcagccgt ggcggcatgc gtgttcgttt ggttgataac ggcgctatcg
    50101 cctttattcc ggcacctttc ttacacgcta tgcgcgatga actggtttgc agccaggaaa
    50161 acggcactgt acaaattaaa ggtgaaacgg tttacaaagt gactgacgtt attgacgtca
    50221 ctattgccga agtccgcatg gaaacccgca gcattattgc gcgcccggtc gcgtaatctc
    50281 ctttcacggc ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat
    50341 ccaagcagat tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta
    50401 caaataaata tataaaaaaa acaatgcgtt tagccgccgt taatttcatg taagtcatgt
    50461 tgttcctttt atttaaccgc ggactccgca gttaaccgga ggatatgcat catgaaaacc
    50521 gttagggagt ccacaacgtt gtacaacttt ctcggatcgc acaatccata ctggcggttg
    50581 acggaaagca gcgatgtttt gcgcttttct accaccgaaa ccacagaacc tgatcgtaca
    50641 ttgcagttat ctgccgaaca ggctgctcgc atcagggaaa tgacggtcat cacctccagc
    50701 ctgatgatga gtctgaccgt cgatgaaagc gatctttctg tgcatctggt aggacgaaaa
    50761 atcaataaac gggaatgggc tggcaacgcg tctgcatggc atgacacacc ggcggttgct
    50821 cgtgatttat cacacgggct ttcctttgct gagcaggtag tttctgaagc acattccgca
    50881 atagtgattc tcgacagccg ggggaatatc caacgcttca atcggttatg tgaagattac
    50941 acagggttga aagaacacga cgtcattggg caaagcgtgt ttaaactgtt tatgagccgt
    51001 cgtgaagctg cggcatccag gcgcaataac cgtgtatttt ttcgaagcgg caatgcatat
    51061 gaagtcgaac tgtggatacc aacatgtaaa ggccagcggc tgtttctgtt tcgcaataaa
    51121 tttgtccaca gcggcagtgg caaaaacgag atttttttaa tctgttccgg caccgacatt
    51181 accgaagagc gccgcgctca ggagcgactg cgtattctgg caaataccga cagtatcacc
    51241 ggactgccga atcgtaacgc aatgcaggat ttaatcgatc acgctattaa tcatgcagat
    51301 aacaataaag ttggggttgt gtatcttgat ttggataatt tcaaaaaggt caacgacgcc
    51361 tatgggcatt tgtttggtga ccagttatta cgcgacgtgt cattggctat tttaagctgt
    51421 ctcgaacatg accaggtgtt ggcgcgtcca ggtggggatg agtttctggt actggcatcc
    51481 aacacctcac aaagcgcgct ggaagcaatg gcatcacgaa ttttgacccg cttacggctc
    51541 ccctttcgca ttggtttaat tgaagtttat accagctgtt cagtaggtat tgcactctct
    51601 cccgaacatg gttcagacag cacggctatt attcgtcacg ccgacacagc aatgtacaca
    51661 gcgaaggaag gcggacgagg acaattttgc gtttttaccc cagaaatgaa tcaacgggta
    51721 tttgaatatc tctggctgga taccaacttg cgtaaagcac tggaaaacga tcagttggtt
    51781 attcactatc aaccgaaaat cacctggcgt ggcgaagtgc gcagtctgga agcactagta
    51841 cgttggcagt cacctgaacg tgggttgatt ccaccgttgg acttcatttc ctacgccgaa
    51901 gagtcagggc taattgtgcc tttaggccgt tgggtgattc tcgatgtcgt acgccaggtg
    51961 gcaaagtggc gggaaaaagg cataaacctg cgagtggcgg taaatatttc tgcacgtcag
    52021 ctcgccgatc aaaccatttt caccgccctg aaacaggttc tccaggaact caattttgaa
    52081 tactgcccta tagatgttga actgacagag agttgtctga ttgagaatga tgaactggca
    52141 ctgtctgtta ttcaacaatt tagccaacta ggtgcgcaag tgcatctgga tgattttggt
    52201 accggctact cttcactttc gcaactggcg cgctttccga tcgatgccat caaacttgac
    52261 caggtttttg ttcgagatat tcacaaacaa cctgtctcgc agtcactggt ccgggcgatc
    52321 gtcgctgtgg cccaggcatt gaatcttcag gtgatcgccg aaggtgtaga gagtgcaaag
    52381 gaagatgctt ttttaaccaa gaacgggatc aatgagcggc aaggattttt gtttgccaaa
    52441 ccgatgcccg ccgtcgcctt cgaacgctgg tataaacgct atctgaagcg cgcataagta
    52501 gatgaatata tatacttagc gctatatccc gtcattatat atcgtattcc tggatgataa
    52561 ttagccgttg ctgttaaaca cttctctttt agcggcaatt tttgcattat tcattttccc
    52621 ccttttgcta taaggatgtc cttgatgtct gaatttgatg cccagcgcgt tgccgaacgt
    52681 attgatattg tgttagatat ccttgtggca ggcgattacc actctgcgat ccataatctc
    52741 gagatactta aagcagaact cttgcgccag gttgctgaat ctacgccaga catcccgaag
    52801 gcaccgtggg agatttaaca atcgtgcctt caggttttcc tatttgagga tgcccggcgc
    52861 gtcagtattt ttttatttag tattataacg ttataagaat tacagcgatg aattcccgac
    52921 aacaaactat tctacagatg gtcattgacc agggtcaggt tagcgtaacc gatctggcaa
    52981 aagccactgg agtttctgaa gttaccattc gccaggatct caacaccctc gaaaaactga
    53041 gttacctccg ccgtgcacat ggctttgcag tttcgcttga tagtgatgac gtcgaaaccc
    53101 gtatgatgag caactatacg ctgaagcgtg aactcgccga gtttgccgcg tctctggttc
    53161 aaccgggcga aaccatcttt atcgaaaatg gtagcagtaa tgccctgctc gcccggactc
    53221 tgggcgaaca gaagaaaaat gtcactatca tcacagtcag cagctacatt gcgcatttgc
    53281 taaaagacgc gccttgtgaa gtcattttgc tcggtggcgt gtaccagaaa aaaagcgaaa
    53341 gtatggttgg ccctttgaca cgccagtgca tccagcaggt acatttcagc aaagcattta
    53401 ttggtattga tggctggcaa cctgaaactg gattcaccgg tcgcgatatg atgcgtaccg
    53461 atgtggtcaa tgccgtgctg gaaaaagagt gcgaagcgat agtcctgact gacagctcga
    53521 aatttggtgc tgtacattca tactccatcg gtcccgttga gcgattcaat cgcgtgatta
    53581 ccgattcgaa aatacgcgcc agcgatctga tgcatcttga gcacagtaaa ctcaccgttc
    53641 acgtcgttga catttaacca tttcccttct ctacggatga tttgcagttt ggcaaatcat
    53701 ccgctctaag atgattcctg gttgataatt aagactattt acctgttatt aacacgctta
    53761 agatataaaa ttattatcag cgatataaca ggaagtcatt atcacctgcg tgatataacc
    53821 ctgcgcgcga gcagatttca cggaataatt tcaccaggct tattcttagc tattatagtt
    53881 atagagagct tacttccgtg aatcataaat tcaggagaga gtattatgtt tgtaacgagc
    53941 aaaaaaatga ccgcggctgt tctggcaatt actttggcaa tgtctctgag tgcctgttct
    54001 aactggtcta aacgggaccg caacaccgca atcggcgcgg gtgcaggggc attaggcggt
    54061 gcagtactga ccgatggcag tacgttgggt acattaggtg gtgcagctgt cggtggtgtt
    54121 attggtcacc aggtcggtaa ataatcgtaa ataaatgtcg attatcgact cgcaattata
    54181 atacgcttga ttctgttcag tacgaaataa ctgaataata cacacagtaa aatattcgaa
    54241 agccacggat atatccgtgg ctttttatgt taaccgcctg cgagttttac cttcatccct
    54301 ttcgcttcaa gtagtgattt taataaatca cgcttatcgc cctggatttc aataactcca
    54361 tctttaactg ctccgccgca gccgcatttt ttcttcagtt ccgctgcaag ttttgtcagt
    54421 tcggcatcat cgagatcgac accggtaatc aggcaaacgc ccttaccttt acgtccactg
    54481 gtctgacgct gaatacgcac cacaccgtcg cctttagggc gtacaggggc cgctttgggc
    54541 tcatcaatac gtccggtttc cgttgagtag accagacggc tgttggaatc actcatcatg
    54601 cactccgctg taaagaggcg ttgatcgctt tcagcgtctg cgctggatct accgattgcg
    54661 ttaccgggcg accaatcacc atataatcaa caccagccga caacgcctgt tctggcgtca
    54721 taatgcggcg ctggtcacca gcttcactcc cctgcggacg aatgcccggc gtaaccagtt
    54781 tgaactcctg accgaatacc tgtttaaagc gcacagcttc ctgagcagaa cacaccacac
    54841 catcaaggcc acatttttgc gtcagtgccg ccagacgttc tgcataatct gcaggtgaca
    54901 gtgtcatgcc aagatcgacc aggtcgctgg cttccatgct ggtcaacact gtcacagcaa
    54961 tcaaaagcgg tgcatctttg ccaaacggaa ccagtgcctc acgcgctgcg gtcatcatac
    55021 gcgccccacc agaggcatga acattcacca tccacacgcc taagtcagct gcagcagcga
    55081 cagcgtgcgc agcagtgtta ggaatatcgt ggaatttcag gtcaagaaag atatcaaaac
    55141 cacgctgttg aagttcgcgc acaaactgtg gcccaaacaa tgtaaacatc tctttgccga
    55201 ccttcagacg acaatcgcgt gggtcgatct tgtcgacaaa ggacagcgcg tcatcacgat
    55261 tatgataatc aagggcaaca accacaggag aattcgtaac agcgcgggaa gaagatgaag
    55321 cagttaacgt catgaccaga ccttcttgat gatgggcacc ggaagtggcg cgaaacaggt
    55381 aaacggcgag cattctaccc gtggaaataa aaaattaaca gttgcgattt ttcccctggc
    55441 aggctttttc tgcgccgtcg ttgccggagc acaatggaac atcaataact agtatgttgt
    55501 aactaaagta ggatttttta aaaattacag gccatcaaga ccgcgaatcg gtttaatggt
    55561 tgaccaggcc cgacaagacg gacaatgcca gtagagggtg tatgcggtaa aaccacattt
    55621 ctggcagcga taacgaggct tactacgtac cttttcgcca accatgtcac gcagcaccat
    55681 caggctctct ttggcacgcc cttcttccgc ttcatttaag tggtaatcca ttaacttatg
    55741 gaacacacgc atggtcggat gacgctgaag ctggcgcgta atatagacct gtgcggcctc
    55801 actaccgtcg cgcgcttcga tgatatcagc aagcatcaat tcagcatcgg caccggtgtt
    55861 ctcttccacc gcgcgttgca ggaattctgc ccattcggca gttttaccca actgctgata
    55921 gcaggtttgc aacatttcca gcgtttcgct gaccagttct ctgtcctggg atatcacgcg
    55981 ttgcaaactt tcgacggctt tggcgtattc tcctttcgcc ataaacacgc gtcccatcat
    56041 gatggatacg cgggcgctgt ttttatctgc cgccgcccct ttttttagca aggtcatggc
    56101 acgatcgaga tcgtcgctgg ccatatgctg cagggctaac tcacagtaga aatgggcaat
    56161 ttcgacgcgc tgtttatctt tacccagctt caccaggcgt tcggcaacat caattgcttt
    56221 ctgccactca ctggtagcct ggtagatttg tagcaactgt tgcagagcgc caatgcggaa
    56281 gtcagtttca tcggtcagct gattgaacat gtcttccgcg cggtcgtata acccggcggc
    56341 catgtaatca cgccctagtt gttgaatcgc caacagacgc tgttcatagg tcagcgaggc
    56401 gctttccatt agggtctgat ggatgcgaat agcgcgatca acttcgccac gcgaacggaa
    56461 caggtttccg agcgtaaggt gggcttcaac ggtgcctgta tcctctttaa gcatatcgag
    56521 aaacaggtct accgctttat cctgttgatt actaagcagg aagttaaccc ccgctacgta
    56581 atcacgcgac aagcggttgg cttcatcttg cttgttttgt tgcgcacttc tgcggcccat
    56641 ataccagcca taggccgcgg ctacaggcaa aagcagaaac aacaactcca gcatagaaag
    56701 ttattccttc gccgctgacg agtgcggcac tacagccacg tcagtcgcgg gtgaaagctg
    56761 gttttccagt cgctttattt tacgttcagc gcgcgccagg gaaacacgaa ctcgcagcca
    56821 gaacaggcca caaatcaacc aaccgatagc aaaccccgca gcaaacaata ccgccagcaa
    56881 tgtggaaata cggtactccc cttgcgctaa cagataatta aacgtcacct gttgatcgtt
    56941 ctgcgcaccc aacgtgaccg aaatcacgaa gatcgctaac accagtaaga aaatgagtaa
    57001 atatttcaca ttacttcccg ttatgtggtt agagcgaata aattatggtc aacttaccgc
    57061 aatagccctt taaagtacca ttttcgcgcc gtctgcgaaa tggcatccac gcgcattgtt
    57121 gatgatttaa gggctaaatt cggaaaatca accagcgtta actttcttgt tctcgttgcg
    57181 ctatttcgcg attttcttcc gcaggtggtg ttaatggccc acaaattcgt tgcgcaagcc
    57241 acgttgccac cgccaccagc gcccacgaaa tcaacgtagc tactaccaga tcgcgtggcc
    57301 aatgcatccc gagcagcagg cggcttccca tgactcccgt tgcccagacc agcaagatag
    57361 caatggttaa cgttcgccga cgcggccaca gcaaaccaac ggccagcagt gcccaactgg
    57421 cagcaaacat cgtgtgaccg gaaggaaagg caaaccccgt ctctttctgc caatgtgaac
    57481 gcaaatattg tgggatattt ttctcttcag ccaactgttc tttcactaga tttccgcgtt
    57541 ctgctcgctt taaagtgtag aactcatcaa ccggaatatg atgtgttttt tccagccaga
    57601 taacaaaagg tcgtggttcc tggactttgt ctttgatcca ggatttaacg ccttgtccca
    57661 caaggattgc ggccgccaga atggcaaata atacaaaggc agccttaatg cgaaaacgca
    57721 gacaccagag aaaccagccg aataaaatca aatgtgtaat gacgccccag ggctgggtga
    57781 cagtttcagt aacccaaaaa gccgctttta gtagccaact ttgttctcca ggttgccaac
    57841 gccagccaga aatccatacg gctactggca tgacaagcaa tagtgcagct cccactgcgg
    57901 tacgtctggc aatcgaacgc atggcctctc ctttttgata agtcccacaa tcataactga
    57961 aaacgccagt tccaggaaaa attgacagat ttgtgccatt ccgtgaacga tcgacgcgtc
    58021 gtgattaggt gaaccccttc tcgttatggc aaaataagcc aatacagaac cagcattatc
    58081 tggagaattt catgcagctt aaacgtgtgg cagaagccaa actgccaacc ccatggggcg
    58141 atttcctgat ggtgggattt gaagaactgg caaccggaca cgatcatgtc gcgctagtct
    58201 atggcgatat ttccgggcat accccggtac ttgcgcgcgt ccattccgaa tgtctgaccg
    58261 gtgacgccct gttcagcttg cgctgcgatt gtggcttcca gctcgaagcg gcattgacgc
    58321 aaattgccga ggaaggccgt ggtattttgc tgtatcaccg tcaggaaggt cgtaacattg
    58381 gtctgctgaa taaaatccgc gcttacgcac tgcaggatca aggttacgat accgtagagg
    58441 ctaaccacca gttaggcttc gccgctgatg agcgcgactt cactctttgc gctgatatgt
    58501 tcaaactcct tggcgtcaat gaagtccgct tgttaaccaa taacccgaaa aaagtcgaaa
    58561 ttctgaccga agcagggatt aatattgttg aacgcgtacc attgattgta ggtcgtaacc
    58621 ccaataacga acattatctc gataccaaag ccgagaaaat gggccatttg ctgaacaaat
    58681 aaccctcttg cattgtgtaa ttaatttgct tgccggaagc aaaataaccg gcaagcaaat
    58741 acttgttact tcaacatatt acgaatgaca taatgcaaaa tgccgtcgtt ctggtagtag
    58801 gtcaactccg tcgcggtgtc gatacgacaa cggcagggta cgacctcctg gctaccatcc
    58861 gcgcgcgtaa gcgtcaccgg aaccgtcgca ccgggttgta gattttgcag atcgccaata
    58921 tcaatcttct cttccccggt tagtcctaac gttttacgcg ttacgccttg cggaaattcc
    58981 agcggcagga tgcccatgcc aattaaattc gaacggtgaa ttcgttcaaa cgattcggca
    59041 atcaccacac gaattccaag cagacgagga ccttttgccg cccagtcacg gcttgagcct
    59101 gatccatact ctttcccggc aatcaccgcc agcggcgttt gttccttctt atagcgcatc
    59161 gcagcatcat aaatagagac tacgtcgctg tcaggtaaat gccgcgtcat ccccccttca
    59221 acgccaggca ccatttcatt acggatgcga atattggcga aggtgccgcg catcatcact
    59281 tcatggttac cacgccgcga accgtaggag ttaaagtctt ttcgctcaac accccgacct
    59341 tgtagatatc gacccgctgg gctgtcgggc ttaatactgc ccgccggaga gatatggtca
    59401 gtggtgactg aatcccccag cattgcgagg atccgcgcac cgtgaatatc ttccactggt
    59461 gctggtgttg cctgcatttc atcaaagaaa ggcgataagc gaatataggt tgagtcctcc
    59521 tgccaaccgt aggtatcgga tcgtgtgacg ttaatttcct tccactctgc tgtgccttca
    59581 aaaacttctg cgtactcttt gcggaacatt tctgtggaga cttgttctac cgcacgggca
    59641 atttcttgtg ccgatggcca gatatctttc agataaaccg ggtcgccttt acgatcatga
    59701 ccaataggct cagcagccag gttgatattc atatttcccg ccagcgcata ggcaaccacc
    59761 agcggcggcg aggccagcca gttagtttta accagcggat ggatacggcc ttcaaagtta
    59821 cggttgccgg acagcaccgc accgacggtt aaatcgcctt ttttgattgc cgtttcgata
    59881 ggatcgggca gcggcccaga gttaccaata caggtggtac aaccgtatcc cacaaggtta
    59941 aaccccagtt cgtcgagata cggtgtcagt ttcgcttttg ccagataatc agaaacgact
    60001 ttcgaacccg gtgccagcga cgctttgacc cacggttgcc gcttgaggcc cagagttacg
    60061 gcttttttcg ccagcaagcc tgcggccatc agcacacttg ggttagaggt gttggtgcac
    60121 gaggttatcg cagcaatgac cacagcgcca tcaggtaact gatactgatg tccgttcata
    60181 acataatcga ccggctggcg atctttatgc gtggcattca cttccagttc gttactggcg
    60241 gcaaatgctt ttggtacatc gggcagtgca acgcgatcct gtgggcgttt tggccctgcc
    60301 aggctcgctt caacgtcatt catatccagt tctaacgtac tggtaaaaat cggttcatcg
    60361 cccgggttac gccacatgcc ctgcgctttg gcatattttt cgaccaactc aacctgatct
    60421 tcgctgcgcc cgcttaaacg catgtaatcg agggttacag catcgattgg gaagaagcca
    60481 caggtggcac catattctgg cgacatattg gcaatggtgg cgcgatccgc cagcggtagt
    60541 gaatccagac catcaccata aaattcgacg aatttcccca ccacgccatg tttgcgcagc
    60601 atttgggtaa cagtgagaac caggtctgtg gcggtaatac cttcacgtaa tttaccggta
    60661 agtttaaagc ccactacgtc cgggataagc atggaaaccg gctggcctaa cattgc
//
LOCUS       DAZFWL020000015        58512 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFWL020000015 DAZFWL020000000
VERSION     DAZFWL020000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 58512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.015"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..428)
                     /gene="folK"
                     /locus_tag="ACHKNP_RS05115"
                     /old_locus_tag="pgaptmp_001023"
     CDS             complement(<1..428)
                     /gene="folK"
                     /locus_tag="ACHKNP_RS05115"
                     /old_locus_tag="pgaptmp_001023"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGU4703234.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGET"
     gene            complement(425..1822)
                     /gene="pcnB"
                     /locus_tag="ACHKNP_RS05120"
                     /old_locus_tag="pgaptmp_001024"
     CDS             complement(425..1822)
                     /gene="pcnB"
                     /locus_tag="ACHKNP_RS05120"
                     /old_locus_tag="pgaptmp_001024"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HGU4703235.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1882..2778)
                     /gene="gluQRS"
                     /locus_tag="ACHKNP_RS05125"
                     /old_locus_tag="pgaptmp_001025"
     CDS             complement(1882..2778)
                     /gene="gluQRS"
                     /locus_tag="ACHKNP_RS05125"
                     /old_locus_tag="pgaptmp_001025"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HGU4703236.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2845..3300)
                     /gene="dksA"
                     /locus_tag="ACHKNP_RS05130"
                     /old_locus_tag="pgaptmp_001026"
     CDS             complement(2845..3300)
                     /gene="dksA"
                     /locus_tag="ACHKNP_RS05130"
                     /old_locus_tag="pgaptmp_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HGU4703237.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3478..4182)
                     /gene="sfsA"
                     /locus_tag="ACHKNP_RS05135"
                     /old_locus_tag="pgaptmp_001027"
     CDS             complement(3478..4182)
                     /gene="sfsA"
                     /locus_tag="ACHKNP_RS05135"
                     /old_locus_tag="pgaptmp_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HGU4703238.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4197..4727)
                     /gene="thpR"
                     /locus_tag="ACHKNP_RS05140"
                     /old_locus_tag="pgaptmp_001028"
     CDS             complement(4197..4727)
                     /gene="thpR"
                     /locus_tag="ACHKNP_RS05140"
                     /old_locus_tag="pgaptmp_001028"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HGU4703239.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4801..7230
                     /gene="hrpB"
                     /locus_tag="ACHKNP_RS05145"
                     /old_locus_tag="pgaptmp_001029"
     CDS             4801..7230
                     /gene="hrpB"
                     /locus_tag="ACHKNP_RS05145"
                     /old_locus_tag="pgaptmp_001029"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HGU4703240.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7362..9896
                     /gene="mrcB"
                     /locus_tag="ACHKNP_RS05150"
                     /old_locus_tag="pgaptmp_001030"
     CDS             7362..9896
                     /gene="mrcB"
                     /locus_tag="ACHKNP_RS05150"
                     /old_locus_tag="pgaptmp_001030"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HGU4703241.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10116..12359
                     /gene="fhuA"
                     /locus_tag="ACHKNP_RS05155"
                     /old_locus_tag="pgaptmp_001031"
     CDS             10116..12359
                     /gene="fhuA"
                     /locus_tag="ACHKNP_RS05155"
                     /old_locus_tag="pgaptmp_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HGU4703242.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLSGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12410..13207
                     /gene="fhuC"
                     /locus_tag="ACHKNP_RS05160"
                     /old_locus_tag="pgaptmp_001032"
     CDS             12410..13207
                     /gene="fhuC"
                     /locus_tag="ACHKNP_RS05160"
                     /old_locus_tag="pgaptmp_001032"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HGU4703243.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13207..14097
                     /gene="fhuD"
                     /locus_tag="ACHKNP_RS05165"
                     /old_locus_tag="pgaptmp_001033"
     CDS             13207..14097
                     /gene="fhuD"
                     /locus_tag="ACHKNP_RS05165"
                     /old_locus_tag="pgaptmp_001033"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HGU4703244.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14094..16076
                     /gene="fhuB"
                     /locus_tag="ACHKNP_RS05170"
                     /old_locus_tag="pgaptmp_001034"
     CDS             14094..16076
                     /gene="fhuB"
                     /locus_tag="ACHKNP_RS05170"
                     /old_locus_tag="pgaptmp_001034"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HGU4703245.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVHAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16111..17391)
                     /gene="hemL"
                     /locus_tag="ACHKNP_RS05175"
                     /old_locus_tag="pgaptmp_001035"
     CDS             complement(16111..17391)
                     /gene="hemL"
                     /locus_tag="ACHKNP_RS05175"
                     /old_locus_tag="pgaptmp_001035"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HGU4703246.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17557..17604
                     /gene="yadX"
                     /locus_tag="ACHKNP_RS05180"
                     /old_locus_tag="pgaptmp_001036"
     CDS             17557..17604
                     /gene="yadX"
                     /locus_tag="ACHKNP_RS05180"
                     /old_locus_tag="pgaptmp_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HGU4703247.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17616..19037
                     /gene="clcA"
                     /locus_tag="ACHKNP_RS05185"
                     /old_locus_tag="pgaptmp_001037"
     CDS             17616..19037
                     /gene="clcA"
                     /locus_tag="ACHKNP_RS05185"
                     /old_locus_tag="pgaptmp_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HGU4703248.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDGARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAMQLARSKAASASENT"
     gene            19061..19126
                     /gene="yadW"
                     /locus_tag="ACHKNP_RS05190"
                     /old_locus_tag="pgaptmp_001038"
     CDS             19061..19126
                     /gene="yadW"
                     /locus_tag="ACHKNP_RS05190"
                     /old_locus_tag="pgaptmp_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HGU4703249.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19119..19463
                     /gene="erpA"
                     /locus_tag="ACHKNP_RS05195"
                     /old_locus_tag="pgaptmp_001039"
     CDS             19119..19463
                     /gene="erpA"
                     /locus_tag="ACHKNP_RS05195"
                     /old_locus_tag="pgaptmp_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HGU4703250.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19510..20133)
                     /gene="yadS"
                     /locus_tag="ACHKNP_RS05200"
                     /old_locus_tag="pgaptmp_001040"
     CDS             complement(19510..20133)
                     /gene="yadS"
                     /locus_tag="ACHKNP_RS05200"
                     /old_locus_tag="pgaptmp_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HGU4703251.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20171..20971)
                     /gene="btuF"
                     /locus_tag="ACHKNP_RS05205"
                     /old_locus_tag="pgaptmp_001041"
     CDS             complement(20171..20971)
                     /gene="btuF"
                     /locus_tag="ACHKNP_RS05205"
                     /old_locus_tag="pgaptmp_001041"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HGU4703252.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20964..21662)
                     /gene="mtnN"
                     /locus_tag="ACHKNP_RS05210"
                     /old_locus_tag="pgaptmp_001042"
     CDS             complement(20964..21662)
                     /gene="mtnN"
                     /locus_tag="ACHKNP_RS05210"
                     /old_locus_tag="pgaptmp_001042"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGU4703253.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21746..23263
                     /gene="dgt"
                     /locus_tag="ACHKNP_RS05215"
                     /old_locus_tag="pgaptmp_001043"
     CDS             21746..23263
                     /gene="dgt"
                     /locus_tag="ACHKNP_RS05215"
                     /old_locus_tag="pgaptmp_001043"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HGU4703254.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23393..24817
                     /gene="degP"
                     /locus_tag="ACHKNP_RS05220"
                     /old_locus_tag="pgaptmp_001044"
     CDS             23393..24817
                     /gene="degP"
                     /locus_tag="ACHKNP_RS05220"
                     /old_locus_tag="pgaptmp_001044"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HGU4703255.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLGDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            24974..26125
                     /gene="cdaR"
                     /locus_tag="ACHKNP_RS05225"
                     /old_locus_tag="pgaptmp_001045"
                     /pseudo
     CDS             24974..26125
                     /gene="cdaR"
                     /locus_tag="ACHKNP_RS05225"
                     /old_locus_tag="pgaptmp_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26214..26600)
                     /gene="yaeH"
                     /locus_tag="ACHKNP_RS05230"
                     /old_locus_tag="pgaptmp_001046"
     CDS             complement(26214..26600)
                     /gene="yaeH"
                     /locus_tag="ACHKNP_RS05230"
                     /old_locus_tag="pgaptmp_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HGU4703256.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26762..27574)
                     /gene="yaeI"
                     /locus_tag="ACHKNP_RS05235"
                     /old_locus_tag="pgaptmp_001047"
     CDS             complement(26762..27574)
                     /gene="yaeI"
                     /locus_tag="ACHKNP_RS05235"
                     /old_locus_tag="pgaptmp_001047"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HGU4703257.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSAGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27629..28453)
                     /gene="dapD"
                     /locus_tag="ACHKNP_RS05240"
                     /old_locus_tag="pgaptmp_001048"
     CDS             complement(27629..28453)
                     /gene="dapD"
                     /locus_tag="ACHKNP_RS05240"
                     /old_locus_tag="pgaptmp_001048"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HGU4703258.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28484..31156)
                     /gene="glnD"
                     /locus_tag="ACHKNP_RS05245"
                     /old_locus_tag="pgaptmp_001049"
     CDS             complement(28484..31156)
                     /gene="glnD"
                     /locus_tag="ACHKNP_RS05245"
                     /old_locus_tag="pgaptmp_001049"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HGU4703259.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31218..32012)
                     /gene="map"
                     /locus_tag="ACHKNP_RS05250"
                     /old_locus_tag="pgaptmp_001050"
     CDS             complement(31218..32012)
                     /gene="map"
                     /locus_tag="ACHKNP_RS05250"
                     /old_locus_tag="pgaptmp_001050"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HGU4703260.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHTVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32380..33105
                     /gene="rpsB"
                     /locus_tag="ACHKNP_RS05255"
                     /old_locus_tag="pgaptmp_001051"
     CDS             32380..33105
                     /gene="rpsB"
                     /locus_tag="ACHKNP_RS05255"
                     /old_locus_tag="pgaptmp_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGU4703261.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33363..34214
                     /gene="tsf"
                     /locus_tag="ACHKNP_RS05260"
                     /old_locus_tag="pgaptmp_001052"
     CDS             33363..34214
                     /gene="tsf"
                     /locus_tag="ACHKNP_RS05260"
                     /old_locus_tag="pgaptmp_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGU4703262.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34361..35086
                     /gene="pyrH"
                     /locus_tag="ACHKNP_RS05265"
                     /old_locus_tag="pgaptmp_001053"
     CDS             34361..35086
                     /gene="pyrH"
                     /locus_tag="ACHKNP_RS05265"
                     /old_locus_tag="pgaptmp_001053"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGU4703263.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35378..35935
                     /gene="frr"
                     /locus_tag="ACHKNP_RS05270"
                     /old_locus_tag="pgaptmp_001054"
     CDS             35378..35935
                     /gene="frr"
                     /locus_tag="ACHKNP_RS05270"
                     /old_locus_tag="pgaptmp_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGU4703264.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36027..37223
                     /gene="ispC"
                     /locus_tag="ACHKNP_RS05275"
                     /old_locus_tag="pgaptmp_001055"
     CDS             36027..37223
                     /gene="ispC"
                     /locus_tag="ACHKNP_RS05275"
                     /old_locus_tag="pgaptmp_001055"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HGU4703265.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37412..38170
                     /gene="ispU"
                     /locus_tag="ACHKNP_RS05280"
                     /old_locus_tag="pgaptmp_001056"
                     /gene_synonym="uppS"
     CDS             37412..38170
                     /gene="ispU"
                     /locus_tag="ACHKNP_RS05280"
                     /old_locus_tag="pgaptmp_001056"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HGU4703266.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNSGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38183..39040
                     /gene="cdsA"
                     /locus_tag="ACHKNP_RS05285"
                     /old_locus_tag="pgaptmp_001057"
     CDS             38183..39040
                     /gene="cdsA"
                     /locus_tag="ACHKNP_RS05285"
                     /old_locus_tag="pgaptmp_001057"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4703267.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39052..40404
                     /gene="rseP"
                     /locus_tag="ACHKNP_RS05290"
                     /old_locus_tag="pgaptmp_001058"
     CDS             39052..40404
                     /gene="rseP"
                     /locus_tag="ACHKNP_RS05290"
                     /old_locus_tag="pgaptmp_001058"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HGU4703268.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40434..42866
                     /gene="bamA"
                     /locus_tag="ACHKNP_RS05295"
                     /old_locus_tag="pgaptmp_001059"
     CDS             40434..42866
                     /gene="bamA"
                     /locus_tag="ACHKNP_RS05295"
                     /old_locus_tag="pgaptmp_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HGU4703269.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42988..43473
                     /gene="skp"
                     /locus_tag="ACHKNP_RS05300"
                     /old_locus_tag="pgaptmp_001060"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42988..43473
                     /gene="skp"
                     /locus_tag="ACHKNP_RS05300"
                     /old_locus_tag="pgaptmp_001060"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HGU4703270.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43477..44502
                     /gene="lpxD"
                     /locus_tag="ACHKNP_RS05305"
                     /old_locus_tag="pgaptmp_001061"
     CDS             43477..44502
                     /gene="lpxD"
                     /locus_tag="ACHKNP_RS05305"
                     /old_locus_tag="pgaptmp_001061"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HGU4703271.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44607..45062
                     /gene="fabZ"
                     /locus_tag="ACHKNP_RS05310"
                     /old_locus_tag="pgaptmp_001062"
     CDS             44607..45062
                     /gene="fabZ"
                     /locus_tag="ACHKNP_RS05310"
                     /old_locus_tag="pgaptmp_001062"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGU4703272.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45066..45854
                     /gene="lpxA"
                     /locus_tag="ACHKNP_RS05315"
                     /old_locus_tag="pgaptmp_001063"
     CDS             45066..45854
                     /gene="lpxA"
                     /locus_tag="ACHKNP_RS05315"
                     /old_locus_tag="pgaptmp_001063"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HGU4703273.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45854..47002
                     /gene="lpxB"
                     /locus_tag="ACHKNP_RS05320"
                     /old_locus_tag="pgaptmp_001064"
     CDS             45854..47002
                     /gene="lpxB"
                     /locus_tag="ACHKNP_RS05320"
                     /old_locus_tag="pgaptmp_001064"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HGU4703274.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46999..47595
                     /gene="rnhB"
                     /locus_tag="ACHKNP_RS05325"
                     /old_locus_tag="pgaptmp_001065"
     CDS             46999..47595
                     /gene="rnhB"
                     /locus_tag="ACHKNP_RS05325"
                     /old_locus_tag="pgaptmp_001065"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGU4703275.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47632..51114
                     /gene="dnaE"
                     /locus_tag="ACHKNP_RS05330"
                     /old_locus_tag="pgaptmp_001066"
     CDS             47632..51114
                     /gene="dnaE"
                     /locus_tag="ACHKNP_RS05330"
                     /old_locus_tag="pgaptmp_001066"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGU4703276.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNRALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51127..52086
                     /gene="accA"
                     /locus_tag="ACHKNP_RS05335"
                     /old_locus_tag="pgaptmp_001067"
     CDS             51127..52086
                     /gene="accA"
                     /locus_tag="ACHKNP_RS05335"
                     /old_locus_tag="pgaptmp_001067"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGU4703277.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52185..54326
                     /gene="ldcC"
                     /locus_tag="ACHKNP_RS05340"
                     /old_locus_tag="pgaptmp_001068"
     CDS             52185..54326
                     /gene="ldcC"
                     /locus_tag="ACHKNP_RS05340"
                     /old_locus_tag="pgaptmp_001068"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HGU4703278.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVALGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54383..54772
                     /gene="yaeR"
                     /locus_tag="ACHKNP_RS05345"
                     /old_locus_tag="pgaptmp_001069"
     CDS             54383..54772
                     /gene="yaeR"
                     /locus_tag="ACHKNP_RS05345"
                     /old_locus_tag="pgaptmp_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4703279.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54837..56135
                     /gene="tilS"
                     /locus_tag="ACHKNP_RS05350"
                     /old_locus_tag="pgaptmp_001070"
     CDS             54837..56135
                     /gene="tilS"
                     /locus_tag="ACHKNP_RS05350"
                     /old_locus_tag="pgaptmp_001070"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGU4703280.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56184..56444)
                     /gene="rof"
                     /locus_tag="ACHKNP_RS05355"
                     /old_locus_tag="pgaptmp_001071"
     CDS             complement(56184..56444)
                     /gene="rof"
                     /locus_tag="ACHKNP_RS05355"
                     /old_locus_tag="pgaptmp_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HGU4703281.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56431..56631)
                     /gene="yaeP"
                     /locus_tag="ACHKNP_RS05360"
                     /old_locus_tag="pgaptmp_001072"
     CDS             complement(56431..56631)
                     /gene="yaeP"
                     /locus_tag="ACHKNP_RS05360"
                     /old_locus_tag="pgaptmp_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HGU4703282.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56797..57342
                     /gene="yaeQ"
                     /locus_tag="ACHKNP_RS05365"
                     /old_locus_tag="pgaptmp_001073"
     CDS             56797..57342
                     /gene="yaeQ"
                     /locus_tag="ACHKNP_RS05365"
                     /old_locus_tag="pgaptmp_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HGU4703283.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57339..57761
                     /gene="arfB"
                     /locus_tag="ACHKNP_RS05370"
                     /old_locus_tag="pgaptmp_001074"
     CDS             57339..57761
                     /gene="arfB"
                     /locus_tag="ACHKNP_RS05370"
                     /old_locus_tag="pgaptmp_001074"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HGU4703284.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57775..58485
                     /gene="nlpE"
                     /locus_tag="ACHKNP_RS05375"
                     /old_locus_tag="pgaptmp_001075"
                     /gene_synonym="cutF"
     CDS             57775..58485
                     /gene="nlpE"
                     /locus_tag="ACHKNP_RS05375"
                     /old_locus_tag="pgaptmp_001075"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HGU4703285.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        13729 a        14535 c        16302 g        13946 t
ORIGIN      
        1 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
       61 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      121 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      181 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgagcgaa
      241 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      301 gtgcggtaaa acgaagaaac ggcaagaatg tggctttcag ggatatcgcc taatgctttc
      361 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      421 actgtcatgc ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacggcgac
      481 gcggtgacgg ctcttcatcc aactcgttaa gcatcccttt ttgatctggt ggcgcggaaa
      541 cctggaactc accccaccat ttcaccagac gctgcagttc agcgttacgt tcaacttcag
      601 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      661 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      721 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      781 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcaatcttc tgtgccgtct
      841 ccagcagtgg gtaccagaac atggcggcaa acaggaacgc cgggttcacg cgcatatcgt
      901 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
      961 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1021 cacacaacag cttataggta tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1081 caaacaggcg tgccggtggg atatcgttca acagggtagc gaggcgaggg atcggttctg
     1141 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1201 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1261 tcagatcctt cataccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataga
     1321 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1381 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1441 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1501 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1561 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1621 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1681 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1741 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1801 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     1861 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     1921 cgactcgggc acggcggtaa gccgccagtt tttgacggct gactgaagga tttgctcgac
     1981 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2041 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2101 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag
     2161 ctggtacagc gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2221 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2281 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgccgcg
     2341 cagctggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcattgtc
     2401 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2461 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2521 acgataggcg tggtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2581 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2641 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga gcctgcaaat agctgccgag
     2701 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2761 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     2821 gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     2881 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     2941 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3001 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3061 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3121 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3181 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3241 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3301 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3361 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3421 aggaaaagcg tatttgcgca cgcgaccaga atgtaaatta accagttact tactttacta
     3481 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3541 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3601 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3661 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3721 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3781 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttctgc
     3841 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     3901 aaggatagcc tctttcgtca acctgttagc ccaaagcgtg ttgacgcaaa taaatgcgcc
     3961 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4021 cgaataccag acggtatcgc caggcgttgc acaaccggtc attgcacccg tattcgggca
     4081 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4141 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4201 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4261 gcgtaaaggg tgaactccgt caccgcatac gaccagttaa aacctggcgg cgggattgtc
     4321 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4381 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4441 ggcggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4501 agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4561 ttctcggcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4621 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccagt ggataatctg ttcgcggatt
     4681 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4741 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4801 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     4861 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     4921 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     4981 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5041 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtagttacc
     5101 gaaggcgtgc tgacgcggat gatccagcgt gacccggagt tgagcggtgt tggattggtg
     5161 atcctcgatg agtttcatga gcgcagcttg caggcggatt tggcgttggc gctgttactc
     5221 gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5281 aacgaccgct tgcagcaaat gctgccagaa gcgcccgtcg tcatctcaga agggcgctcg
     5341 tttccggttg aacgccgtta tttaccactg cccacgcatc agcgttttga cgaagccgtt
     5401 gcggtagcca ccgctgaaat gctgcgtcag gaaagtggat cattactgtt atttttacct
     5461 ggcgtcggag aaattcagcg tgtgcaggaa caactggctt cgcgcatcgg cagcgatgta
     5521 ttgctctgcc cgctgtatgg cgcgttgtcg ctgaacgatc agcgaaaagc gatcctcccg
     5581 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5641 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ttttgatccg
     5701 cgcacggggc ttacgcgact gattactcaa cgcgttagcc aggcatccat gacgcagcgt
     5761 gccgggcgcg ccgggcgtct ggagccgggt atctgcctgc atttaatcgc caaggaacaa
     5821 gcagaacgcg ccgcggcgca aagtgaaccg gagatattac aaagcgatct ttccggtttg
     5881 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     5941 ccgccagtag tgaatctact ggccgcgaaa cgtctgttac aaatgctggg ggcactggag
     6001 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6061 gcggcaatgc tggttagcgc gaagaacgac gacgaagctg ctaccgcggc aaaaattgcc
     6121 gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6181 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgtggc
     6241 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6301 gctcgtcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc
     6361 gatgccaacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6421 agcgcctcgc cggatgcgcg gattttactg gcgctgccgg tcgatattga tgagttagta
     6481 caacgctgcc cgcagctggt acagcagtct gacactgtgg agtgggatga cgcgcaaggt
     6541 acgctgaaag cctggcgtcg gctgcaaatc ggtctgctga cggtgaaagt gcagccgctg
     6601 gcgaaaccct cggaagacga gttgcatcag gcgatgctta atggcatccg cgataaagat
     6661 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6721 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt attggcgacg
     6781 ctggaaacgt ggctgctgcc acatatgact ggcgtacatt cactacgcgg cctgaaatca
     6841 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     6901 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     6961 gaagataacc cgcccgcgct ggcggtgaga atgcaagaga tgtttggcga ggccaccaat
     7021 ccgacgatcg cccaggggcg cgtgccgctg gtgctggagt tgctttcacc tgcccaaagg
     7081 ccattacaaa tcacacgaga tttgagcgcc ttctggaaag gagcgtaccg tgaggtgcaa
     7141 aaagagatga aagggcgtta tcccaaacat gtctggccgg acgacccggc aaatactgca
     7201 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7261 ggggaccgat cgagcacaaa ttttgagaga tatcttcttc tgtcttgtaa cagaagaaca
     7321 gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag catggccggg aatgaccgcg
     7381 agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa acaaaaggta agccgtcgtc
     7441 gttacgaaga tgacgatgat tacgacgatt atgatgacta tgaggatgaa gaaccgatgc
     7501 cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg tggcaaacgc ggctggctat
     7561 ggctgctgtt aaaactggct atcgtttttg ccgtgctgat cgccatttac ggcgtttatc
     7621 tcgatcaaaa aattcgtagc cgtattgatg gcaaggtctg gcaactgcct gcggcagttt
     7681 atggccgaat ggtcaacctt gagccagata tgaccatcag caagaacgag atggtgaagc
     7741 tgctggaggc gactcagtat cgtcaggtgt cgaaaatgac ccgtcctggc gaatttaccg
     7801 tgcaggccaa cagcattgag atgatccgcc gtccgtttga tttcccggac agtaaagaag
     7861 ggcaggtgcg tgcgcgtctg acctttgatg gcgatcatct ggcgacgatc gtcaatatgg
     7921 agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg tctgatcacc atgatctcct
     7981 cgccaaacgg tgagcagcgt ttgtttgtgc cgcgcagtgg tttccctgat ttgctggtgg
     8041 atactttgct ggcgacagaa gaccgtcatt tttacgagca tgacggcatc agcctctact
     8101 ccatcggacg tgcggtgctg gcaaacctga ccgccggacg cacggtgcag ggagcgagta
     8161 cgctgacgca acagctggtg aaaaacctgt tcctctccag tgagcgttct tactggcgta
     8221 aagcgaacga agcttacatg gcgctgatca tggacgcgcg ttacagcaaa gaccgtattc
     8281 ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg cgacaacgaa atccgcggct
     8341 tcccgctggc gagcttgtat tactttggtc gcccggtaga agagctaagc ctcgaccagc
     8401 aggcgctgtt agtcggcatg gtgaaaggag cgtccatcta caacccgtgg cgtaacccaa
     8461 aactggcgct ggagcgacgt aatctggtgc tgcgtctgct gcaacagcaa cagattattg
     8521 atcaagaact ctatgacatg ttgagtgcac gtccgctggg tgttcagcca cgcggtgggg
     8581 tgatctctcc acagccagcc tttatgcaac tggtgcgtca ggagctgcag gcgaaattag
     8641 gcgataaggt aaaagatctc tccggcgtga agatcttcac tacctttgac tcggtggccc
     8701 aggacgcggc agaaaaagcc gccgtggaag gcattccggc actgaagaaa cagcgtaagt
     8761 tgagcgatct tgaaactgcg attgtggtcg tcgaccgctt tagtggtgaa gttcgtgcga
     8821 tggtcggtgg ttctgagccg cagtttgcgg gctacaaccg tgcgatgcag gcgcgtcgtt
     8881 cgattggttc ccttgcaaaa ccagcgactt atctgactgc cttaagccag ccgaaaatct
     8941 atcgtctgaa tacgtggatt gcggatgcgc caatcgcgct gcgtcagccg aatggccagg
     9001 tctggtcacc gcagaatgat gaccgtcgtt atagcgaaag cggcagagtg atgctggtgg
     9061 atgcgttgac ccgttcgatg aacgtgccga cggtaaatct ggggatggcg ctggggctgc
     9121 ctgcggttac ggagacctgg atcaaactgg gcgtaccgaa agatcagttg catccggttc
     9181 cggcgatgct gctgggggcg ttgaacttaa cgccaatcga agtggcgcag gcgttccaga
     9241 ccatcgccag cggcggtaac cgtgcaccgc tttctgcact gcgttcggta attgcggaag
     9301 atggcaaggt gctgtatcag agcttcccgc aggcggaacg cgctgttccg gcgcaggcgg
     9361 cgtatctgac actatggacc atgcagcagg tggtacaacg cggtacgggt cgtcagcttg
     9421 gggcgaaata cccgaacctg catctggcag ggaaaacagg gactaccaac aataacgtag
     9481 atacctggtt tgcaggcatt gacggcagca cggtgaccat cacctgggta ggccgtgata
     9541 acaaccagcc gaccaaactg tatggagcca gcggggcaat gtcgatttat cagcgttatc
     9601 tggctaacca gacgccaacg ccgctgaatc ttgttccgcc agaagatatt gcagatatgg
     9661 gcgtggacta cgacggcaac tttgtttgca gcggtggcat gcgtgtcttg ccggtctgga
     9721 ccagcgatcc gcaatcgctg tgccagcaga gcgagatgca gcagcagccg tcaggcaatc
     9781 cgtttgatca gtcttctcag ccgcagcaac agccgcaaca gcaacctgct cagcaagagc
     9841 agaaagacag cgacggtgta gccggttgga tcaaggatat gtttggtagt aattaacatc
     9901 taagcgtgaa ataccggatg gcgagttgcc atccggtaaa ataacatccc atctaagata
     9961 ttaacccttt cttttcatct ggttgtttat taacccttca ggaacgctca gattgcgtac
    10021 cgcttgcgaa cccgccagcg tttcgaatat tatcttatct ttataataat cattctcgtt
    10081 tacgttatca ttcactttac atcagagata taccaatggc gcgttccaaa actgctcagc
    10141 caaaacactc actgcgtaaa atcgcagttg tagtagccac agcggtaagc ggcatgtctg
    10201 tttatgcaca ggcagcggtt gaaccgaaag aagacactat caccgttacc gctgcacctg
    10261 cgccgcaaga aagcgcatgg gggcctgctg caactattgc ggcgcgacag tctgctaccg
    10321 gcactaaaac cgatacgccg attcaaaaag tgccacagtc tatttctgtt gtgaccgccg
    10381 aagagatggc gctgcatcag ccgaagtcgg taaaagaagc gcttagctac acgccgggtg
    10441 tctctgttgg tacgcgtggc gcatccaaca cctatgacca cctgatcatt cgcggctttg
    10501 cggcagaagg ccaaagccag aataactatc tgaatggcct gaagttgcag ggcaacttct
    10561 ataacgatgc ggtcattgac ccgtatatgc tggaacgcgc tgaaattatg cgtggcccgg
    10621 tttccgtgct ttacggtaaa agcagtcctg gcggcctgtt gaatatggtc agcaagcgtc
    10681 cgaccaccga accgctgaaa gaagttcagt ttaaagccgg tactgacagc ctgttccaga
    10741 ctggttttga ctttagcgat tcgttggatg atgacggtgt ttactcttat cgcctgaccg
    10801 gtcttgcgcg ttctgccaat gcccagcaga aagggtcaga agagcagcgt tatgctattg
    10861 caccggcgtt cacctggcgt ccggatgata aaaccaattt caccttcctt tcttacttcc
    10921 agaacgagcc ggaaaccggt tattacggct ggttgccgaa agagggaacc gttgagccgc
    10981 tgccgaacgg taagcgtctg ccgacagact ttaatgaagg ggcgaagaac aacacctatt
    11041 ctcgtaatga gaagatggtc ggctacagct tcgatcacga atttaacgac acctttactg
    11101 tgcgtcagaa cctgcgcttt gctgaaaaca aaacctcgca aaacagcgtt tatggttacg
    11161 gcgtctgctc cgatccggcg aatgcttaca gcaaacagtg tgcggcatta gcgccagcgg
    11221 ataaaggcca ttatctggca cgtaaatacg tcgttgatga tgagaagctg caaaacttct
    11281 ccgttgatac ccagttgcag agcaagtttg ccactggcga tatcgaccac accctgctgt
    11341 ccggtgtcga ctttatgcgt atgcgtaatg acatcaacgc ctggtttggt tacgacgact
    11401 ctgtgccact gctcaatctg tacaatccgg tgaataccga tttcgacttc aatgccaaag
    11461 atccggcaaa ctccggccct taccgcattc tgaataaaca gaaacaaacg ggcgtttatg
    11521 ttcaggatca ggcgcagtgg gataaagtgc tggtcaccct aggcggtcgt tatgactggg
    11581 cagatcaaga atctcttaac cgcgttgctg ggacgaccga taaacgtgat gacaaacagt
    11641 ttacctggcg tggtggtgtt aactacctgt ttgataatgg tgtaacacct tacttcagct
    11701 atagcgaatc gtttgaacct tcttcgcaag ttgggaagga tggtaatatt ttcgcaccgt
    11761 ctaaaggtaa gcagtatgaa gtcggcgtga aatatgtacc ggaagatcgt ccgattgtag
    11821 ttactggtgc cgtgtataat ctcactaaaa ccaacaacct gatggcggac cctgagggtt
    11881 ccttcttctc ggttgaaggt ggcgagatcc gcgcacgtgg cgtagaaatc gaagcgaaag
    11941 cggcgctgtc ggcgagtgtt aacgtagtcg gttcttatac ttacaccgat gcggaataca
    12001 ccaccgatac tacctataaa ggcaatacgc ctgcacaggt gccaaaacac atggcttcgc
    12061 tgtgggctga ctataccttc tttgacggtc cgctttcagg tctgacgctg ggcaccggtg
    12121 gtcgttatac tggctccagc tatggtgatc cggctaactc ctttaaagtg ggaagttata
    12181 cggtcgtgga tgcgttagtg cgttatgatc tggcgcgagt cggcatggcg ggctccaacg
    12241 tggcgctgca tgttaacaac ctgttcgatc gtgaatacgt cgccagctgc tttaacactt
    12301 atggctgctt ctggggcgca gaacgtcagg tcgttgcaac cgcaaccttc cgtttctaat
    12361 ttctcttttg gggcacggat ttccgtgccc atttcacaag ttggctgtta tgcaggaata
    12421 cacgaatcat tccgatacca cttttgcact gcgtaatatc tcctttcgtg tgcccgggcg
    12481 cacgcttttg catccgctgt cgttaacctt tcctgccggg aaagtgaccg gtctgattgg
    12541 tcacaacggt tctggtaaat ccactctgct caaaatgctt ggccgtcatc agcctccgtc
    12601 agaaggggag attcttcttg atgcccagcc gctggaaagc tggagcagca aagcgtttgc
    12661 ccgcaaagtg gcttatttgc cgcagcagct tcctccggca gaagggatga ccgtgcgtga
    12721 actggtggcg attggtcgtt acccgtggca tggcgcgctg gggcgctttg gtgcggcaga
    12781 tcgcgaaaag gtcgaggaag ctatctcgct ggttggctta aaaccgctgg cgcatcggct
    12841 ggtcgatagt ctctctggcg gcgaacgtca gcgggcgtgg atcgccatgc tggtggcgca
    12901 ggatagccgt tgtctgttgc tcgacgaacc gacttcggcg ctggatatcg cccaccaggt
    12961 tgatgtgctg gcgctggtgc accgtttaag tcaggaacgt ggcctgacgg tcattgccgt
    13021 gctgcacgat atcaatatgg cggcacgcta ctgtgattat ctggtcgccc tgcgcggtgg
    13081 tgaaatgatt gctcagggaa cgcctgcgga aattatgcgc ggcgaaaccc tcgaaatgat
    13141 ttatggcatc ccgatgggta ttttgccgca tccggcgggt gctgcacctg tgagttttgt
    13201 ttattgatga gcggcttacc tcttatttcg cgccgtcgac tgttaacggc gatggcgctt
    13261 tctccgttgt tatggcagat gaataccgcc cacgcggcgg ctattgaccc caatcgtatt
    13321 gtggcgctgg agtggttgcc ggtggaatta ctgctggcgc tcggcatcgt gccttacggc
    13381 gtggcggata ccatcaacta tcgcctgtgg gtcagcgaac caccattgcc ggactcagtg
    13441 attgacgtcg gtttgcgtac agaacctaac cttgaactgc tgaccgaaat gaaaccgtcg
    13501 tttatggtct ggtcggcagg atatggccct tcaccagaaa tgctggcacg tattgcgccg
    13561 ggtcgcggat ttaacttcag tgacggcaaa cagccgttgg cgatggcgcg taaatcgctg
    13621 acggaaatgg cagatttact taacctgcaa agcgcagcag aaacgcattt agcgcactat
    13681 gaagacttta tccgcagcat gaaaccccgc tttgtgaagc gtggtgcacg cccgttactg
    13741 ctgacgacgc ttatcgatcc gcgccatatg ctggtcttcg gtccaaacag cttgttccag
    13801 gaaattctcg atgagtacgg catcccaaat gcctggcaag gggaaaccaa cttctggggc
    13861 agtaccgccg tcagtatcga tcgtctggcg gcgtataaag acgttgatgt gctctgtttt
    13921 gatcacgaca acagcaaaga catggatgcg ctaatggcaa ccccgctgtg gcaggccatg
    13981 ccgtttgtcc gtacagggcg ctttcagcgc gtacccgcag tctggtttta tggtgcaacg
    14041 ctttcggcaa tgcactttgt gcgtgttctg gataacgcca tcggaggtaa agcgtgagta
    14101 aacgaattgc gcttttcccg gcgttattgc tggcgctgtt agtgattgtc gctacggcgc
    14161 taacctggat gaacttctca caggcgctgc cgcgcagtca gtgggcgcag gccgcctggt
    14221 cgccggatat tgacgtcatc gagcagatga tttttcacta cagcttgttg ccgcgtctgg
    14281 cgatttcgct gctggtgggc gcaggcctgg ggctggtggg cgtgctgttt cagcaagtgc
    14341 tgcgtaatcc gctggcggaa ccgacgacac ttggcgttgc tacaggcgcg caactgggaa
    14401 ttaccgtcac cacactttgg gcgatcccag gtgctatggc gagccagttt gccgcactgg
    14461 tgggcgcttg tgtcgttggc ttaatcgtct ttggcgtcgc gtgggggaaa cggctttcgc
    14521 cggttacgct tattctcgcg gggctggtgg tgagccttta ttgcggggca attaatcagc
    14581 tactggttat cttccatcac gaccagctgc aaagcatgtt cctgtggagc accggaacgc
    14641 tgacgcaaac cgactggggc ggcgttgagc gtttatggcc gcagttgctg ggcggcgtga
    14701 tgttgacgtt attgctactt cgcccgttaa ccctgatggg gcttgatgat ggcgtggcgc
    14761 gcaatctcgg gctggccttg tcgctcgcgc gtctggcagc gctgtcgctg gcgattgtca
    14821 tcagtgcgct gctggtgcac gctgtgggga ttatcggctt tatcgggttg ttcgcgccgc
    14881 tgctggcaaa aatgctgggg gcgcggcgtc tgctgccacg actgatgctg gcgtcgctga
    14941 ttggggcgct gatcctctgg ctttccgatc aaatcatcct ctggctgact cgcgtgtgga
    15001 tggaagtgtc caccggttcg gtcactgcgt tgatcggtgc gccgctgcta ctgtggctgt
    15061 tgccgcgttt acgcagcatt agcgcgccgg atatgaaggt caacgatcgt gtcgcggctg
    15121 aacgccaaca tgtgctggcg tttgccctcg cgggcggcgt gctgctgttg atggctgtgg
    15181 tggtggcgct gtcgtttggt cgtgatgcgc acggctggac gtgggcgagc ggggcgttgc
    15241 ttgaggattt gatgccctgg cgctggccgc gaattatggc ggcgctgttt gcgggcgtca
    15301 tgctggcggt ggcgggctgt attattcagc ggctgaccgg aaacccgatg gcaagcccgg
    15361 aagtgctggg gattagctct ggcgcagcgt ttggcgtggt gttgatgctg tttctggtgc
    15421 cgggtaatgc ctttggctgg ctgttacctg caggcagtct cggcgcggcg gtgacgctgt
    15481 tgatcattat gatcgccgcc ggccgcggtg gattttcccc acaccgcatg ttactggcgg
    15541 ggatggcgtt aagcaccgcg ttcaccatgc ttttgatgat gttgcaggca agtggtgacc
    15601 cgcgaatggc gcaagtgctg acctggattt ccggttcgac ctacaacgcg accgatgcgc
    15661 aggtctggcg cacgggaatt gtgatggtga ttttgctggc gattaccccg ctgtgccgcc
    15721 gctggctgac cattttaccg ctgggtggtg ataccgcccg agccgtagga atggcgctga
    15781 cgccgacgcg aattgcgctg ctgctgttag cggcttgcct gacggcgacc gcgacgatga
    15841 ctattggacc gttgagtttt gttggtttaa tggcaccgca tattgcgcgg atgatgggct
    15901 ttcgacggac gatgccacac atcgtaattt cggcgctggt gggtggttta ctgctggtgt
    15961 tcgctgactg gtgtgggcgg atggtgctgt ttccattcca gatcccagca gggctgctgt
    16021 cgacctttat cggcgcgcca tattttatct atttgttgag aaagcagagc cgttaataga
    16081 atgcctcgcc cggtgggcga ggcaaaatca tcacaacttc gcaaacaccc gtcgtgcagc
    16141 atcgatggtg ttattgatat cttccatgct gtgcgccacg gacataaagc ccgcttcaaa
    16201 cgctgacggt gccaggtaaa caccttcgtc cagcatcata tggaagaaac gcttaaagcg
    16261 ttccacgtca caggccatca catcctgata gcacgtcacg gactcggcgt cggtaaagaa
    16321 aataccgaac atgccgccaa cgtggttaac gaccagcgga attccggctt cttctgccgc
    16381 ttccagcaga ccttctgcca gacgtgttgt cagctcatcc agcgtttcgt gaacgcccgg
    16441 ctgcgcgact tcattcagac aggcgaaacc cgctgccatc gcaattgggt taccggaaag
    16501 cgtacccgcc tgatagaccg gacccgtcgg ggccagcgca tccattacat cacgacgacc
    16561 accgaatgcg cctaccggca ttccaccgcc gatgattttg cccaggcagg tgagatccgg
    16621 ttccacaccg taataatcct gtgcgccagc tagcgctacg cggaagccag tcattacttc
    16681 atcgatgatc agcaatgcgc caaattcgtc gcacagcgcg cgcagacctg gcaggaactc
    16741 tggcagcggt ggaacgcagt tcatattgcc tgccaccggc tcgacgataa tacaggcaat
    16801 ctcttgcggg tattgctcaa acgcggcgcg tacagaagcc agatcgttat aagtacaggt
    16861 taaggtatgt ttggcgaaat ctgccggaac acccggcgag tttggctggc ctaacgtgag
    16921 tgcgccagaa ccggctttca ccagcaggca gtcagcgtga ccgtggtaac aaccttcaaa
    16981 tttaatgatt ttgtcgcgac cggtaaaacc acgggccagg cggatggcgc tcatcgtcgc
    17041 ttcagtgccg gagttcacca tgcgcaccat atccatggtc gggaccagtt cggtcaccag
    17101 ttgcgccatt ttcacttcca tttcggtcgg tgcaccaaag cttaaaccac gctcggcggc
    17161 ttcaatcacg gcattgcgga tagccggatg gttatggccc agcaccatcg gcccccagga
    17221 accgacataa tcgatatagg ctttgccatc aacatcgtac agataagcgc cgtccgcttt
    17281 ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg cgaacagggg agttcacacc
    17341 gccagggatc agctcgcgcg ctgcgctgta aagattttca gacttactca tggagggttc
    17401 ctgattcgta gaaaaagtga atggctgcta ttctatgtta ttcataacaa gttaaatact
    17461 cgtcaaacat caggctgctt gtactggtgc aatcctgatt tcttagagta taaaagtttt
    17521 gtgcatttga aacattacgc tttgcaaagg attttcatgg aacgtgcgag taaaatgccg
    17581 tcatcttatt tgtatgacca ataagtgatc attggatgaa aactgatact ccctctttag
    17641 aaacaccgca ggccgcgcgc ctgcgacgca gacaactgat tcgccaactt cttgagcgcg
    17701 ataaaacccc gttagccatt ttgtttatgg cggcagtcgt cggcacgctt gtcgggctgg
    17761 cagcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa ccaacgtatg ggggcgctgg
    17821 tacatactgc tgataattat ccgcttctgt taaccgtcgc ttttctctgt tcggcggtgc
    17881 tggcgatgtt cggctacttt ttggtgcgca aatacgcgcc ggaagcaggt ggttcgggga
    17941 tcccggaaat tgaaggggcg ctggaagatc aacgtcccgt tcgctggtgg cgtgtgttgc
    18001 cggtgaaatt ctttggcggg ctggggacac tcggcggagg catggtgcta gggcgcgaag
    18061 gaccaaccgt gcagatcggc ggcaatatcg gtcgcatggt gcttgatatt ttccgcctga
    18121 aaggtgatgg agctcgccat acgctgctgg caaccggtgc tgctgcgggg ctggctgcgg
    18181 cctttaacgc gccgctggcg ggtattttgt ttattatcga agagatgcgt ccgcagtttc
    18241 gctatacgtt aatttcgatt aaagcggtat ttattggcgt cattatgtcg accattatgt
    18301 accggatttt taatcatgaa gttgcgttga ttgacgtcgg taaactttct gatgcgccgc
    18361 ttaatacgct gtggctttat ctgatcctcg gtattatttt tggcattttc ggccctattt
    18421 ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg tgtgcacggc ggcaatatta
    18481 ccaaatgggt actgatgggt ggtgcgattg gcggtctgtg tggattgctg gggtttgtgg
    18541 caccagcaac gtcgggcggc ggttttaacc tgattcctat cgctaccgcg gggaatttca
    18601 gcatgggaat gctggtgttt attttcgtcg ctcgggtcat taccacctta ctctgcttct
    18661 cttccggcgc gccgggcggt atttttgccc cgatgctggc gctgggtact gtgctgggaa
    18721 ccgctttcgg aatggttgcc gttgagctgt ttccgcaata tcacctggag gcggggacgt
    18781 ttgctattgc cggaatgggg gcattactgg cggcatctat tcgcgcgccg ttaacgggga
    18841 tcattctggt tctggagatg accgataact accagctcat tttgccaatg attattaccg
    18901 gtcttggcgc aacactatta gcgcaattta ccggcgggaa accgctatac tcggcgattc
    18961 ttgcgcgcac gctggcaaaa caggaagcta tgcaactggc gcgaagcaag gccgcatcag
    19021 ccagcgagaa tacttgaacg aaataccagg gtattagata atggcgatta ttattgggtt
    19081 agaatttgcc caattgccga tgtcgtttgg agcaaaatat gagtgatgac gtagcactgc
    19141 cgctggagtt taccgacgca gcagccaaca aagttaaaag cctgatcgct gacgaagata
    19201 acccgaatct gaaattacgc gtgtatatca ccggtggcgg ttgcagcggc ttccagtatg
    19261 gtttcacctt tgatgatcag gtgaacgaag gcgatatgac catcgaaaaa cagggcgttg
    19321 gcttggtggt tgatccgatg agcctgcaat atctggtcgg cggttccgtt gactataccg
    19381 aaggactgga aggttctcgt ttcatcgtga ccaacccgaa cgcgaaaagc acctgcggtt
    19441 gcggttcttc ctttagcatc taatctgttg ttcatgtgcc ggataaacta tccggcacat
    19501 cttctctctt caacgcccat tctcatccag cgcaaacgtc ggtagcttaa gatgccaacg
    19561 aatagccgcc agccgaatca agagcgtcac gaccatgccc atcatactgg ctgtttccag
    19621 tggtacggaa aatgtgtaat aagccgtagc gtggacaata ccgccgataa tacaggcagt
    19681 tgcgtagatt tctgtacgta aaatcatggg gatttcgcgg gccagaacat cacgaatgat
    19741 cccgccgcca acgccagtaa tgacgcccat acaaaccgcg attaacggac cggcttccgc
    19801 attaaaggct ttattcacgc caatgccgac aaacaccgcc agaccaacgg cgtccagcac
    19861 cggcaacatc cattttggta agcgtcttgg ctggcgcacc agcacgatgg tcagcatgct
    19921 ggttaccatt gcaacgacca gatcggtggg atctttcacc caaaataccg ggccgtgatc
    19981 cagcgccatg tcgcgaattg tcccgccgcc tacagcggta accacgccca gtaccagaac
    20041 accaaaaggg tccatacgca attttccggc taacaaaacg ccggagatgg caaataccgc
    20101 tgtgccgact atatccagcc aatagacgag cattgttcaa tccccactga gcacctgaaa
    20161 aggtcaggca ctaatctacc tgtgaaagcg cattacagag ctgttgtgca gcgaggataa
    20221 tacgtgggct tgcacgttca aaccagtcac tcgtgagagg aataacggga attttgagct
    20281 gttcacccca gtattgtttg attttaggaa tttggtccgg tccgcctgta atgacaatcg
    20341 cctgtggcga gcgtgctaac acctgttcgc ggctaacttg cggccaggga acccggctgt
    20401 ctttaaagat gttttctccg ccacaaactt cgagtacctg gttctgaatc gactcttttc
    20461 cactggtaaa tggcggatta atgccgaatt gcagaaaaac acgtttttta ggtttatcag
    20521 catattgcgc tttcaattgc gcgtactgat ccagcaggga ttgcgcggct tgttcggcct
    20581 tgtctggttg cggactccag ggggccagtt gacgtaacgc attggcaatt tgttcaatgc
    20641 ttgtcgcatc gacccacatc acttttattc ccagcgaagc cagctggtca acctgccgct
    20701 cggcattacc tccacgccag gcaatcacca gatcgggttt cagcgcgaca atgcgttcca
    20761 gattcatccc ctgccaggtg gaaacctgct caatcttttg cgcttgtgga ggatagtcgg
    20821 aatagctgct gaccccaacc ggcgtgatcc cggcggcaaa ggcaagttca gtgttggcgg
    20881 gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag tggcgcaaga aaagacaggg
    20941 cgaccagcgc cctgaacagt gacttagcca tgtgcaagtt tctgcaccag tgactcaacc
    21001 atcaggctgg actgtttagc ggcaacagcc aggaactcat cgaagctaag atgagactgt
    21061 tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg ggacgttgaa attgtggcag
    21121 acatgggcga ttgccgtcgc ttccatctct acagcaatgg cctgtgggaa gttgtggcgg
    21181 attttcgcca gaccaacaga accgttgatg aaagcgtcgc cgctaacaat caggccacgt
    21241 acagcgttaa gattcagttc ggcaatgcag gcctcagcgg cagcgatcag tttatcgtca
    21301 gctttaaagc ctgccggaca gcctggtaac tgaccgtatt cataaccaaa tgccgtgaca
    21361 tccgcgtcgt gataacgtgc ttcgtccgag acaacgatat cgcccacttt caacgttggt
    21421 gccaggccac cggcagaacc ggtgttaata atcacatctg gcttgcagtg ttccaacagc
    21481 aaagtggcac ccagcgccgc agcgacttta ccgatgcccg atttcagaag cgcaacctcg
    21541 gttccattca gttggccggt atagatttcg caaccgccga gactgatagt ttgacgtttt
    21601 tcgattttgt cacgcagcag cgtaacttct tcttccattg caccaatgat gccgattttc
    21661 atagatttac tcgcgataag cccgatttga aggcatagtt taccatgcgc ttacggggaa
    21721 gcgtatttct cacgcgggag aggacatggc acagattgat ttccgaaaaa aaataaactg
    21781 gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa catgagatcc tgcggatctt
    21841 cgagagcgat cgcgggcgta tcatcaactc tccggcaatt cgtcgtctgc aacaaaagac
    21901 gcaggttttt ccactggagc gcaatgccgc cgtgcgcacg cgtcttaccc actcgatgga
    21961 agtccagcag gtggggcgct acatcgccaa agaaatttta agccgtctga aagagcttaa
    22021 attgctggaa gcatacggcc tggatgaact gaccggacct tttgaaagca ttgttgagat
    22081 gtcatgcctg atgcacgata tcggcaatcc gccgtttggt cattttggcg aagcagcgat
    22141 aaatgactgg tttcgccaac gtttgcaccc ggaagatgcc gaaagccagc ctctgactga
    22201 cgatcgctgc agcgtggcgg cactacgttt acgggacggg gaagaaccgc ttaacgagct
    22261 gcggcgcaag attcgtcagg acttatgtca ttttgagggg aatgcacaag gcattcgtct
    22321 ggtgcataca ttgatgcgga tgaatctcac ctgggcacag gttggcggta ttttaaaata
    22381 tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca catcactatt taatgaaaaa
    22441 gccgggttat tatctttctg aagaagccta tattgcccgg ttgcgtaaag aacttaattt
    22501 ggcgctttac agtcgttttc cattaacttg gattatggaa gctgccgacg acatctccta
    22561 ttgtgtggca gaccttgaag atgcggtaga gaaaagaata tttaccgttg agcagcttta
    22621 tcatcatttg cacgaagcgt ggggccagca tgagaaaggt tcgctctttt cgctggtggt
    22681 tgaaaatgcc tgggaaaaat cacgctcaaa tagtttaagc cgcagtacgg aagatcagtt
    22741 ttttatgtat ttacgggtaa acaccctaaa taaactggta ccctacgcgg cacaacgatt
    22801 tattgataat ctgcctgcga ttttcgccgg aacgtttaat catgcattat tggaagatgc
    22861 cagcgaatgc agcgatcttc ttaagctata taaaaatgtc gctgtaaaac atgtgtttag
    22921 ccatccagat gtcgagcagc ttgaattgca gggctatcgg gtcattagcg gattattaga
    22981 gatttatcgt cctttattaa gcctgtcgtt atcagacttt actgaactgg tagaaaaaga
    23041 acgggtgaaa cgtttcccta ttgaatcgcg cttattccac aaactctcga cgcgccatcg
    23101 gctggcctat gtcgaggctg tcagtaaatt accgtcagat tctcctgagt ttccgctatg
    23161 ggaatattat taccgttgcc gcctgctgca ggattatatc agcggtatga ccgacctcta
    23221 tgcgtgggat gaataccgac gtctgatggc cgtagaacaa taaccaggct tttgtaaaga
    23281 cgaacaataa atttttacct tttgcagaaa ctttagttcg gaacttcgcg ctataaaacg
    23341 aatctgaaga acacagcaat tttgcgttat ctgttaatcg agactgaaat acatgaaaaa
    23401 aaccacatta gcactgagtg cactggctct gagtttaggt ttggcgttat ctccgctctc
    23461 tgcaacggcg gctgagactt cttcagcaac gacagcccag cagatgccaa gccttgcacc
    23521 gatgctcgaa aaggtgatgc cttcagtggt cagcattaac gtagaaggta gcacaaccgt
    23581 taatacgccg cgtatgccgc gtaatttcca gcagttcttc ggtgatgatt ctccgttctg
    23641 ccaggaaggt tcaccgttcc agagctctcc gttctgccag gggggccagg gcggtaatgg
    23701 cggcggccag caacagaaat tcatggcgct gggttccggc gtcatcattg atgccgataa
    23761 aggctatgtc gtcaccaaca accacgttgt tgataacgcg acggtgatta aagtccagct
    23821 gggcgatggt cgtaagttcg acgcgaagat ggttggcaaa gatccgcgct ctgatatcgc
    23881 gctgattcag atccagaacc cgaaaaacct gaccgcaatt aagatggcgg attctgatgc
    23941 actgcgcgtg ggtgattaca ccgtagcgat tggtaacccg tttggcctgg gagagacggt
    24001 aacttccggg attgtctctg cgctggggcg tagcggcctg aatgccgaaa actacgaaaa
    24061 cttcatccag accgatgcag cgatcaaccg tggtaactcc ggtggtgcgc tggttaacct
    24121 gaacggcgaa ctgatcggta tcaacaccgc gatcctcgca ccggacggcg gcaacatcgg
    24181 tatcggtttt gctatcccga gcaacatggt gaaaaacctg acctcgcaga tggtggaata
    24241 cggccaggtg aaacgcggtg agctgggtat tatggggact gagctgaatt ccgaactggc
    24301 gaaagcgatg aaagttgacg cccagcgcgg tgctttcgta agccaggttc tgcctaattc
    24361 ctccgctgca aaagcgggca ttaaagcggg tgatgtgatc acctcactga acggtaagcc
    24421 aatcagcagc tttgccgcac tgcgtgctca ggtgggtact atgccggtgg gcagcaaact
    24481 gaccctgggc ttgctgcgcg acggtaagca ggtcaacgtg aatctggaac tgcagcagag
    24541 cagccagaat caggttgatt ccagctccat cttcaacggc attgaaggcg ctgagatgag
    24601 caacaaaggc aaagatcagg gcgtggtagt gaacaacgtg aaaacgggca ctccggctgc
    24661 gcagatcggc ctgaagaaag gtgatgtgat tattggcgcg aaccagcagg cagtgaaaaa
    24721 catcgctgaa ctgcgtaaag ttcttgacag caaaccgtct gtactggcac tgaacattca
    24781 gcgcggcgac agctccatct acctgttaat gcagtaatct ccctcaaccc cttcctgaaa
    24841 acgggaaggg gttctcctta caatctgtga acttcaccac aactccatac atcttcatca
    24901 tcctttaggc atttgcacaa tgccgtacgt tacgtacttc cttatgctaa gccgtgcata
    24961 acggaggact tatggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac
    25021 catgcgcatc atcgatacca atatcaacgt aatggatgcc cgtgggcgaa ttatcggcag
    25081 cggcgatcgt gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg
    25141 acgagtcgtc gatatcgatg acgcggtggc acgtcatctg cacggtgtgc ggcaagggat
    25201 taatctaccg ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc
    25261 agagaatctg cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca
    25321 gtcgcggttg atgcacttgt tggctcagga tagccgtttg cgggaggaac tggtgatgaa
    25381 cctgattcag gcagaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat
    25441 cgatctcaat caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggtgt
    25501 ggacagcgca atggcggagt tacaacaact gcaaaacgcg ctgactacgc ccgagcgtaa
    25561 taatctggtg gcgattgtct cgctaaccga aatggtggtg ttgaaaccgg cgttgaactc
    25621 ttttgggcgc tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat
    25681 gaaagagtac ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg
    25741 cagtattgcc cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat
    25801 gccagaaagt cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt
    25861 gcgtggcgac tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga
    25921 caataacggc ttgctgcgac gaacgctggc ggcgtggttt cgtcacaatg tgcaaccgct
    25981 ggcaacgtca aaggcgttgt ttattcatcg taataccctg gagtatcggc ttaatcgtat
    26041 atcggaactg accgggcttg atttgggcaa ttttgatgac aggttgctgc tgtatgtggc
    26101 gttacaactg gatgaagagc ggtaggttat gcgttaaggt tgaggcggcg ctggctcatt
    26161 cccctcagcc cgaaggggag aggggaatgc ccgaattaac cgtcaattcg ggattattta
    26221 cgcgttagtt tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc
    26281 aggtgacgta agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg
    26341 cagatttgat caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct
    26401 ttataaccct gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac
    26461 ttaacgctct tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg
    26521 ttgttggctt cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga
    26581 cttttcagat tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt
    26641 agcatttggc ctggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac
    26701 ttctatcatg gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt
    26761 tctacaccag ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac
    26821 tgcccacgcc acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt
    26881 aacgtttatc ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc
    26941 cgtgagtatg gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt
    27001 cgggattatg cgccagcacc agtcttggca gattggcttc gctggcggga ggcggtttgc
    27061 attgtccggc ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga
    27121 tcaccgtagc ctggttaaac aacaccgtga tgcccgctga tttcaacgtc tcgccaatta
    27181 agtgattttt ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg
    27241 cacattcggc aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa
    27301 ataatacgta atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa
    27361 tcgcgtcaga aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa
    27421 gaattttgaa tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa
    27481 accagcctgg ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg
    27541 cagtagcctg caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg
    27601 cagcgtcgtg ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc
    27661 cgactttgcc gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt
    27721 atttgccatc ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac
    27781 cgtagtggat ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc
    27841 ccatggaaat gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc
    27901 caatgaagca gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc
    27961 cgatgcccac gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg
    28021 tcgcccaggt atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg
    28081 gcatcagcac ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa
    28141 cgcggaagcc ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt
    28201 tgtcgaagta gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga
    28261 gcagcaccgc ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta
    28321 cacgcagtgc accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg
    28381 tgtctgcgtt ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct
    28441 gtaactgctg cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg
    28501 gattgagggc ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac
    28561 gccggtcagc ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc
    28621 gggcaccatg aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc
    28681 ctggctggtc gagcgcgata agctcaagga acgatttgcg gtcggtatgg gtcggcaaaa
    28741 acgttacttc ggtttcaaca gtaaaatggc gtaatttggc gggttggcga cggggctgcg
    28801 gtggctgcca gctactttgc gtcagtactt gctccagacc aaaccgaata acctcatgac
    28861 gatctgcgga cagcgggctg ccatcgggtt ccagcacgat aaaggtatcc atcgccatac
    28921 cgtcgcgagt ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg
    28981 cacagacggc ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc
    29041 cacgcgtagc ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat
    29101 ggcgggcatg ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc
    29161 gtgaccaaat ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg
    29221 cctggagttg gtgatgacga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc
    29281 gtagctgctt ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc
    29341 acagcgtttc gttggtggcg caaatgtcag ccaccgtcag gcataccaga taacgaagtc
    29401 ggttttctgt ttgcacttct tcggcaaact gcttgatgac ttccgggtcc tgaatatcgc
    29461 ggcgttgggc ggtcaccgac atcaacaggt gctggcgaac cagccaggca accagctgtg
    29521 tttcgcgcga gttaagcccg tggagttcgg caaaatgcac tacatcctga gcaccgagaa
    29581 tggagtggtc gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca
    29641 gctcagttga cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt
    29701 cttcactggc aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt
    29761 aggcgtggaa cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg
    29821 cgccgagcac gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac
    29881 gcagaatgct caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat
    29941 ggcgacgggc atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt
    30001 tgcgcaccat ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt
    30061 catcacgcag gtcgattagc gtaccgcgta gctgaaactc atcgtcgagt ggacgtggtt
    30121 tttcgtcggc gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga
    30181 gttcactgac gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt
    30241 tgccttcacc actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacagac
    30301 gattatcgta acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca
    30361 gacattcgtt taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat
    30421 ccagcgatgt tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca
    30481 agccgccagg gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct
    30541 gatggcgctg gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc
    30601 cttcgctaaa aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc
    30661 gggattcgat taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt
    30721 caagcgtgcg cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct
    30781 cgcccacttt ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga
    30841 tgtcagaaag tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt
    30901 cggcaatctg gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact
    30961 cggtgcgcgc ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc
    31021 aacgctggaa agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc
    31081 agacgcatgg attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt
    31141 ctggaagggt attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg
    31201 cattcgccgg cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg
    31261 cgtagcgtca gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca
    31321 gacaagctgc gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt
    31381 ttacccgcgt tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg
    31441 gtttcacggg agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg
    31501 tgtccgcaat attcacgaac gacggagaag ccttctgctt cgacaaattt ctgaatcgcc
    31561 gcaccgattt cgcgcagatt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt
    31621 tcttgcgtga tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt
    31681 ttcgaggtat cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg
    31741 ccatctttca gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag
    31801 atgcaaacgg atttcggata gccgtgatag ccgaggcagg cagaaaccgt gtgttgttca
    31861 ttaacaatgt aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca
    31921 tacggttcga tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg
    31981 atatcttctg gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac
    32041 cgacaatata tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc
    32101 tgaatttcgc caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc
    32161 tgtacacagc gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg
    32221 acttccgatc catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac
    32281 aacacacacg tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg
    32341 tggaggcata accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc
    32401 gacatgctca aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg
    32461 aagccgttca tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta
    32521 ccgatgttca acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc
    32581 cttttcgttg gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc
    32641 gaccagttct tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt
    32701 cgtcagtcca tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa
    32761 aagctgacca agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc
    32821 ctgggcggta tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac
    32881 cacgaacaca ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt
    32941 gataccaact ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc
    33001 cgtgctgtga ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag
    33061 gatctggctt cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact
    33121 cccccaaaat agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca
    33181 tcagtaagtg accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg
    33241 gggagagccc ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc
    33301 tttttcactt ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta
    33361 gaatggctga aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca
    33421 tgatggattg caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa
    33481 acatgcgtaa gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg
    33541 gcgtgatcaa aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga
    33601 ctgacttcgt tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag
    33661 ctgttgctgg caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg
    33721 ttgcgctggt agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag
    33781 gcgacgttct gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag
    33841 gcgctgacga agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat
    33901 tcatcaaacc agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg
    33961 atatcgcgat gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca
    34021 tgaagaaatt caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca
    34081 aaactgttgg tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg
    34141 aagtgggtga aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt
    34201 ccaagcagtc ttaattatca aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc
    34261 ttgtaaattc agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt
    34321 attaattcat ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc
    34381 cgtctataaa cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt
    34441 cggtattgat gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact
    34501 gggtattcag gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc
    34561 gaaagcgggt atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa
    34621 cggcctggca atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc
    34681 tattccattg aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg
    34741 caacaaccgt gtggtgatcc tctctgccgg tacaggtaac ccgttcttta ccaccgactc
    34801 agcagcttgc ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt
    34861 tgacggcgtg tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact
    34921 gacttacagc gaagtgctgg aaaaagagct gaaagtcatg gacctagcgg ccttcacgct
    34981 ggctcgtgac cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg
    35041 ccgtgtggta atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat
    35101 aaataagggt aagattccgc gtaagtatcg cgggggcgtt agtctggtta taaggcgtta
    35161 ttgttgcagg cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg
    35221 aggatggcga gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg
    35281 attacccgta atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt
    35341 ctggtctgac tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga
    35401 tgctgaagta cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg
    35461 cacgggtcgt gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc
    35521 gacgccgctg cgtcagctgg caagcgtaac ggtagaagat tcccgtacac tgaaaatcaa
    35581 cgtgtttgat cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg
    35641 cctgaacccg aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga
    35701 acgtcgtaaa gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt
    35761 acgtaacgtg cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat
    35821 cagcgaagac gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat
    35881 caagaaaatt gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct
    35941 tgaacgacaa aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt
    36001 tattctgtct caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct
    36061 cgattggttg cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg
    36121 cgctggtggc aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc
    36181 gctatgccgt aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac
    36241 agggtagccg caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg
    36301 aggatgttga tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg
    36361 ctgcgatccg cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg
    36421 gacgtctgtt tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg
    36481 aacataacgc catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg
    36541 accttgagca aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg
    36601 agacgccatt gcgcgatttg gcaacaatga cgccggatca agcctgccgt catccgaact
    36661 ggtcgatggg gcgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat
    36721 acattgaagc gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc
    36781 cgcagtcagt gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg
    36841 gggaaccgga tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact
    36901 ctggcgtgaa gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt
    36961 atgatcgtta tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga
    37021 cgacagcatt gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc
    37081 gctttacgga tatcgctgcg ttgaatttat ccgtactgga aaaaatggat atgcgcgaac
    37141 cacaatgtgt ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag
    37201 aggtgatgcg tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag
    37261 cgtagggctt cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt
    37321 caggtatgcc gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc
    37381 gtaaaccctg tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga
    37441 aaaattgcca gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg
    37501 ggcaaaaaag caagggaaga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg
    37561 ccgggctgtc tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag
    37621 tagtgaaaac tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg
    37681 ggcgctcgat agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg
    37741 cgataccagt cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac
    37801 agccgggaat agcggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat
    37861 agtccaggga gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca
    37921 gatagatgaa gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt
    37981 agtaattagg actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta
    38041 tgccgaactt tactttacag atgttctctg gcccgatttc gatgaacaag actttgaagg
    38101 ggcgttaaat gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga
    38161 aacagcctga tgggggtcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat
    38221 acccgtcgtc atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct
    38281 ggtggtctgc atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc
    38341 gcagcgagta tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt
    38401 gccggaatat caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt
    38461 gggttggtgg attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg
    38521 gcgtaactct aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg
    38581 gggcatgctg gcgttacggg cctggcacta cgacgagaat cattacagtg gcgcaatatg
    38641 gctgctctat gtcatgatcc tggtatgggg cgctgactct ggcgcatata tgtttggcaa
    38701 attgtttggt aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt
    38761 tataggtgga ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct
    38821 cgacgtcgct cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct
    38881 cggcgatctg accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt
    38941 aattccagga cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt
    39001 ctttgcttgc ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt
    39061 tttctctggg atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa
    39121 tttggtcatt tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg
    39181 tttggtaagg cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatcgccctg
    39241 atcccgctgg gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa
    39301 ctccgccacc atgccttcaa taataaatct gtcggccaac gagcggcgat tattgccgca
    39361 ggtccggttg caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt
    39421 gtgcctggcg tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca
    39481 caaattgcac caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat
    39541 gccgtgcgtt tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg
    39601 ccatttggca gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag
    39661 cctgataaag aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa
    39721 cctgtactgg aaaatgtgca accaaactcg gcggcaagca aggcaggttt gcaagcaggc
    39781 gacaggatcg ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt
    39841 gtccgggata acccgggtaa atccttagcg ttagaaatcg aaaggcaggg aagtcccttg
    39901 tctttgacat taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt
    39961 attgagccga aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg
    40021 ttcaacgcca tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt
    40081 atgctgggaa aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct
    40141 atcgccaagg gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt
    40201 gcgcttatta gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg
    40261 gggcatctgc tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt
    40321 caagactttt gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt
    40381 ttcaatgatt tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga
    40441 tgaaaaagtt gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag
    40501 ggttcgtagt gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggccc
    40561 tcctcagtat gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca
    40621 ttcgcgctct gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata
    40681 cccttctggt tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca
    40741 aatcggtgaa agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg
    40801 aatccctcga tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca
    40861 gcgtcggtaa atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg
    40921 ttgacctaaa actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg
    40981 ttggtaacca tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag
    41041 tgccgtggtg gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc
    41101 ttgaaaccct gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta
    41161 cccaggtcag tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag
    41221 gcgatcagta caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg
    41281 aaattgagca gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca
    41341 agatggaaga tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac
    41401 agtcgatgcc tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg
    41461 cgggtaaccg tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg
    41521 ccgtcctgcg tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg
    41581 atcagggtaa ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc
    41641 aacgtgttcc gggtagcccg gaccaggtag atgtcgtcta caaggtaaaa gagcgcaaca
    41701 ccggtagctt caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg
    41761 gtgtgcagca ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa
    41821 acgattacca gacctatgct gaactgtcgg taaccaaccc gtacttcact gtagatggtg
    41881 taagcctcgg tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg
    41941 actataccaa caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata
    42001 actcgctgcg tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg
    42061 ttgcgatgtg gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata
    42121 atagcttcaa aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc
    42181 gtggttactt cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg
    42241 gatcggataa cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg
    42301 acgatcacaa atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg
    42361 gcaaagagat gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct
    42421 tccagtccaa tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg
    42481 atccggacta tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg
    42541 atgatgctgt aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc
    42601 cgtttattag cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta
    42661 ccgtttggga tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc
    42721 caagcaatat ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt
    42781 tggtgttctc ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc
    42841 agtttaacat cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag
    42901 cgatgacttt aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg
    42961 tgcaaatggg atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt
    43021 ttagcactgg caacttctgc tcaggcggct gacaaaattg caatcgtcaa catgggcagc
    43081 ctgttccagc aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa
    43141 ggccgtgcca gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg
    43201 cagtccatga aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc
    43261 cagacttttg ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa
    43321 gaacgcggca aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag
    43381 gatatcgatc tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac
    43441 atcactgccg acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat
    43501 ttagcgcagc agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt
    43561 gcgtccatgc aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt
    43621 gagcatttag gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc
    43681 gcgaaaagtg ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa
    43741 attttagata ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg
    43801 acggcgaagc tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt
    43861 gaactgggcg ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc
    43921 ggtgcaggtt cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag
    43981 aattgcctga tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat
    44041 cgtggtaact gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag
    44101 atcggtgcct gcacaaccat cgatcgcggc gcgctggatg acactattat tggcaatggc
    44161 gtgatcattg ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg
    44221 gttgccggtg gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc
    44281 ggagccagcg taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg
    44341 ggtatggtga tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa
    44401 cccaacaaag tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag
    44461 cgtctgaaat cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg
    44521 ccaaacttta cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt
    44581 tcttatattt tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag
    44641 agattttaga acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt
    44701 ttgaagaagg tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc
    44761 agggccattt ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac
    44821 aggcaacagg tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact
    44881 acttcgctgg tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga
    44941 tcatggaagt cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc
    45001 tggtcgatgg taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct
    45061 gatacgtgat tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt
    45121 caattggcgc gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg
    45181 gtgagggtac cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg
    45241 ataatgagat ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg
    45301 gcgaaccgac ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc
    45361 atcgtggcac agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga
    45421 tcaacgcgca cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg
    45481 caacgctggc gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag
    45541 tccatcagtt ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc
    45601 aggacgtccc tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata
    45661 tcgaagggct gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt
    45721 ataagctgat ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac
    45781 tggcggaaac atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg
    45841 gtctgattcg ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac
    45901 ctccggcgat atcctggggg ccggtttaat ccgcgctctg aaagaacgtg tgcccaacgc
    45961 ccgctttgtt ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cctggtacga
    46021 aatggaagaa ctggcggtga tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact
    46081 gcatattcgt gccgatctga caaagcgttt tggcgaactg aagccagata tttttgttgg
    46141 tattgatgcg cctgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa
    46201 aaccattcat tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat
    46261 aggcagagcc accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa
    46321 atacaatgta ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc
    46381 agataaaaat gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt
    46441 gctaccaggg agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc
    46501 ccagcttttg cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa
    46561 acgccgcgag cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt
    46621 gctggatggg atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg
    46681 tacggcagcc ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa
    46741 gccttttacc ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa
    46801 tctgctggcg ggtagagagt tagtcaaaga attattgcag gaagagtgtg agccgcaaaa
    46861 actggctgcg gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga
    46921 taccttccgt gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc
    46981 cgttctggag ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt
    47041 gtggatgaag tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt
    47101 gacccggcgc gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt
    47161 ctggcgctct atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa
    47221 ccccacgaaa tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc
    47281 gtcgctgggc tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa
    47341 ttaccgatgc ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc
    47401 gcgtctatcc tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc
    47461 ccgcaatatg gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg
    47521 gctgaacacg gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg
    47581 ggacttgcgt cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa
    47641 ccacgtttcg tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa
    47701 accgcaccgt tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat
    47761 ttcaccaacc tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag
    47821 cctatcgtcg gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac
    47881 ctgacggtac tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa
    47941 gcgtatcagc gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa
    48001 ttaaacgaag ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt
    48061 ttgcgtggta acagagcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg
    48121 gatcgctatt ttctcgagct gatccgcacc ggcaggccgg atgaagaaag ctatctgcac
    48181 gcggcggtgg aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc
    48241 tttatcgaca gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt
    48301 accctcgacg atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa
    48361 gaggagatgt gtgagctgtt tgccgacatc cccgaagccc ttgccaacac cgttgagatc
    48421 gccaaacgct gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc
    48481 ggggacatga gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt
    48541 ctggcctttt tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa
    48601 cgtctggaga ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc
    48661 gttatggaat ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc
    48721 tccggtgcgg gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa
    48781 tttgacctgc tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac
    48841 gttgacttct gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt
    48901 cgtgatgcgg tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc
    48961 gacgtaggcc gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc
    49021 ccgcccgatc cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa
    49081 atctacgaag cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg
    49141 gtcacccgta acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc
    49201 gattttgcgc cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa
    49261 agcgacgttg aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc
    49321 atcatcaact gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg
    49381 ctggatatcg ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg
    49441 gaaaccacgg cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtcta
    49501 caacctgact gcttcgaaga tatgatcgcc ctagtggcac tgttccgccc cggtccgttg
    49561 caatcaggga tggtggataa ctttatcgac cgtaaacatg gtcgtgaaga gatctcctat
    49621 ccggacgtac agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatt
    49681 atcctgtatc aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt
    49741 ggcgcggata tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcaa
    49801 cgttctgtat ttgctgaagg tgcagaaaag aacggaatca acgctgaact ggcgatgaaa
    49861 atcttcgacc tggtggagaa attcgctggt tacggattta acaaatcgca ctctgcggcc
    49921 tatgctttgg tgtcatatca aacgttatgg ctgaaagcac actatcctgc ggagtttatg
    49981 gcggcggtaa tgaccgccga tatggacaac accgagaagg tggtgggtct ggtggatgag
    50041 tgctggcgga tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc
    50101 cacgtcaacg acgacggcga aatcgtgtat ggtattggcg cgatcaaagg ggtcggtgaa
    50161 ggtccgattg aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt
    50221 gatctctgcg cccgtaccga caccaaaaag ttgaaccgtc gcgtgctgga aaaactgatc
    50281 atgtccgggg cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt
    50341 gatgcgttaa aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg
    50401 ttcggcgtgc tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg
    50461 tggccggagc aggtggtatt agatggggaa cgtgaaacgt taggcctgta cctgaccgga
    50521 caccctatca accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa
    50581 gacatgcacc cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg
    50641 cgggttatgg tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc
    50701 gggcggctgg aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa
    50761 aaagaccgca tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt
    50821 aaaatgaccg ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg
    50881 cttgctatct cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag
    50941 tctctggaac cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat
    51001 gcacgcgcgc ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta
    51061 aacgatctcc gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg
    51121 aatactatga gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa
    51181 atcgattctc tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa
    51241 gaagtgcatc gtctgcgtga aaaaagcgta gaactgacgc gtaaaatctt cgccgatctt
    51301 ggtgcatggc agattgcgca actggcacgc catccacagc gtccttatac cctggattac
    51361 gttcgcctgg catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat
    51421 aaagctatcg tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat
    51481 caaaaaggtc gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa
    51541 ggttaccgca aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc
    51601 acctttatcg acaccccggg ggcttatccg ggcgtgggcg cagaagaacg tggtcagtct
    51661 gaagccattg cacgcaacct gcgtgaaatg tctcgcctcg gtgtaccggt agtttgtacg
    51721 gttatcggtg aaggtggttc tggcggcgcg ctggcgattg gcgtgggcga taaagtgaat
    51781 atgctgcaat acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg
    51841 aagagcgctg acaaagcgcc gctggcagct gaagcgatgg gtatcatcgc tccgcgtctg
    51901 aaagaactaa aactgatcga ctccatcatc ccggaaccgc tgggtggtgc tcaccgtaac
    51961 ccggaagcga tggcggcatc gttgaaagcg caactgctgg cggatctggc cgatctcgac
    52021 gtgttaagca ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac
    52081 gcgtaattcg caaaagttct gaaaaagggt cacttcggtg gccctttttt atcgccacgg
    52141 tttgagcagg ctatgattaa ggaaggattt tccaggagga acacatgaac atcattgcca
    52201 ttatgggacc gcatggcgtc ttttataaag atgagcccat caaagaactg gagtcggcgc
    52261 tggtggcgca aggctttcag attatctggc cacaaaacag cgttgatttg ctgaaattta
    52321 tcgaacataa ccctcgaatt tgcggcgtga tttttgactg ggatgagtac agtctcgatt
    52381 tatgcagcga tatcaatcaa cttaatgaat atctcccgct ttatgccttc atcaacaccc
    52441 actcgacgat ggatgtcagc gtgcaggata tgcggatggc gctctggttt tttgaatatg
    52501 cgctggggca ggcggaagat atcgccattc gtatgcgtca gtacaccgac gaatatcttg
    52561 ataacattac accgccgttc acgaaagcct tgtttaccta cgtcaaagag cggaagtaca
    52621 ccttttgtac gccggggcat atgggcggca ccgcatatca aaaaagcccg gttggctgcc
    52681 tgttttatga ttttttcggc gggaataccc tcaaggctga tgtctctatt tcggtcaccg
    52741 agcttggttc gttgctcgac cacaccgggc cacacctgga agcggaagag tacatcgcgc
    52801 ggacttttgg cgcggaacag agttatatcg ttaccaacgg aacatcgacg tcgaacaaaa
    52861 ttgtgggtat gtacgccgcg ccatccggca gtacgctgtt gatcgaccgc aattgtcata
    52921 aatcgctggc gcatctgttg atgatgaacg atgtagtgcc agtctggctg aaaccgacgc
    52981 gtaatgcgtt ggggattctt ggtgggatcc cgcgccgtga atttactcgc gacagcatcg
    53041 aagagaaagt cgctgccacc acgcaggcac aatggccggt tcatgcggtg attaccaact
    53101 ccacctatga tggcttgctc tacaacaccg actggatcaa acagacgctg gatgtcccgt
    53161 cgatccactt cgattccgcc tgggtgccgt acacccattt tcatccgatc taccagggga
    53221 aaagtggtat gagcggcgag cgtgttgcgg gaaaagtgat cttcgaaacg caatcgaccc
    53281 acaaaatgct ggcggcgtta tcgcaggctt cgctgatcca cattaaaggc gagtatgacg
    53341 aagaggcgtt taacgaagcc tttatgatgc ataccaccac ctcgcccagt tatcccattg
    53401 ttgcttcggt tgagacggcg gcggcgatgc tgcgtggtaa tccgggcaaa cggctgatta
    53461 accgttcggt ggagcgggcg ctgcattttc gcaaagaggt ccagcggttg cgggaagagt
    53521 ctgacggttg gtttttcgat atctggcaac cgccgcaggt ggatgaagcc gaatgctggc
    53581 ccgttgcgct aggcgaacag tggcacggct ttaacgatgc ggatgccgat catatgtttc
    53641 tcgatccggt taaagtcact attttgacac cggggatgga cgaacagggc aatatgagcg
    53701 aggaggggat cccggcggcg ctggtggcaa aattcctcga cgaacgtggg atcgtagtag
    53761 agaaaaccgg cccttataac ctgctgtttc tctttagtat tggtatcgat aaaaccaaag
    53821 caatgggatt attgcgtggg ttgacggaat ttaaacgctc ttacgatctc aacctgcgga
    53881 tcaaaaatat gctacccgat ctctatgctg aagatcccga tttctaccgc aatatgcgta
    53941 ttcaggatct ggcgcaaggg atccataagc tgattcgtaa acacgatctt cccggtttga
    54001 tgttgcgggc attcgatact ttgccggaaa tgatcatgac gccacatcag gcatggcaga
    54061 gacagattaa aggtgaagta gaaaccattg cgctggaaca actggtcggt agagtatcgg
    54121 caaatatgat cctgccttat ccaccgggcg taccgctgtt gatgccagga gaaatgctga
    54181 ccaaagagag ccgcacggta ctcgattttc tactgatgct ttgttccgta gggcaacatt
    54241 accccggttt tgaaacggat attcacggcg cgaaacagga cgaagacggc gtttaccgcg
    54301 tacgagtcct aaaaatggcg ggataacttg ccagagcggc ttccgggcga gtaacgtgct
    54361 gttaacaaat aaaggagacg ttatgctggg tttaaaacag gttcaccata ttgcgattat
    54421 tgcgacggat tatgcggtga gcaaagcttt ctactgcgat attcttggtt tcacgctgca
    54481 aagcgaagtc tatcgcgaag cgcgcgactc gtggaaaggg gatttggcgc ttaatgggca
    54541 atatgtgatt gagcttttct cttttccgtt cccgccggag cgccccagcc gaccggaagc
    54601 ttgcggcctg cgtcatctgg cttttagcgt tgatgacatc gatgcggcag tggcgcacct
    54661 tgaaagccat aacgtgaagt gtgaagccat ccgtgtcgat ccatacacgc aaaaacgctt
    54721 caccttcttt aacgatccgg acgggctgcc gttggaactg tatgagcagt aaggcttgtc
    54781 atcgccgcat ttgcccggta acgtgccggg cattgctact gtaaaatcgc accatcatga
    54841 cactcacgct caatagacaa cttctcacct cacgccagat tctggtggcc tttagcggcg
    54901 ggcttgactc caccgttctg ctgcatcagt tggtgcagtg gcggacggaa aatccgggcg
    54961 tcactctgcg cgctattcat gtgcatcacg gtttaagtgc caatgccgat gcctgggtta
    55021 cgcattgcga aaacgtctgc caacagtggc aggtgccgct ggtggtcgaa cgcgtgcaac
    55081 ttgcgcaaga aggactgggc attgaggccc aggcgcggca ggcgcgttat caggcatttg
    55141 cccgcacctt gttgcccggt gaagtgctgg tcaccgcgca acatctcgac gatcaatgtg
    55201 aaacttttct gctggcgcta aaacgcggta gcggccctgc cgggctttcg gctatggcgg
    55261 aagtttcgga gtttgccgga acacggctta tccgcccgtt gctcgcccgc acgcggggag
    55321 aactggagca gtgggcattg gcgcatggtt tacgctggat tgaagacgaa agtaatcagg
    55381 acgactcata cgatcgtaac tttctgcgcc tgcgcgtagt gccgttattg cagcagcgtt
    55441 ggccgcattt tgcggaagca acggcccgca gcgccgcact ttgtgctgaa caagagagtc
    55501 tgttggatga actgctggcg gatgatttag cacactgcca aacgtcgcag gggacgctgc
    55561 agattgcgcc aatgctggcg atgagtgatg cccgccgcgc ggcgattatc cgtcgctggc
    55621 tggcagggca gaatgcacca atgccttccc gcgacgcgct ggtgcggatc tggcaggaag
    55681 tggcgctggc gcgggaagat gcctccccct gtttacgctt gggcgcgttt gaaatccgcc
    55741 gctatcagtc gcaactgtgg tggattaaat ccgtcaccgg gcaaagtgaa accattgtgc
    55801 cgtggcagac gtggctacaa ccgctggaat taccggcggg gctgggaagc gtacagctta
    55861 ctgcgggagg cgatattcgc cctccgcgtg cagacgaagc ggtcagcgtg cgtttcaaag
    55921 cggcaggatt gctgcatatt gtcgggcgca acggcggacg taagctgaag aaaatctggc
    55981 aagagttagg cgtgccgccg tggctacgtg acaccacgcc actgctgttt tatggcgaaa
    56041 cgctgattgc ggcggcaggg gtatttgtga cgcaagaagg tgtggctgaa ggtgagaatg
    56101 gcgtaagttt tgtctggcag aaaacgctta gttaagtgaa agccggataa gacgcatcaa
    56161 acgtcgcatc cggcgaaagt caatcaggac tcgctcacca ccaccgtacc gatttccggg
    56221 tggctaaagc tggtaatttt atccagacgt agctcacggg tttcgccagc ggcctcgacg
    56281 accagatact ccacattttt gcgggagact aaatcgctgg cttttgcctg caatttttcg
    56341 ccatctttca gctcaagtgt cagcattaaa tgatgctggc aggcgagctc aagattatcg
    56401 taatcatcac aattgattgg ttgatacgta tcattcattg acataatcgc tcaccagtaa
    56461 gtttgccgca gcgtatgctg ctttttccct gacagcctca gaaagggcgt cgtcggcagc
    56521 catttcattc agcactttca aaacgcagcc cagcgcgtcc ggaacgtatc ctaagtctcc
    56581 gctggcgatt tccgcgtacc gcttgcgtat taactcacaa tatttttcca catgccctcc
    56641 tgtcagcact ctgacttaac cgtggatgca agtctaagcc tacgaagata aactctgttt
    56701 cgcaaggtga ctataccaca ctcatgtctg caatatcagc gccgcaactg cacgtattcc
    56761 gttacaatgg cctcctgatt cgaaaggagt tttcttatgg cgcttaaagc gacaatttat
    56821 aaagcgacgg ttaatgtggc cgatctcgac cgcaaccagt ttctcgatgc ctctctgacg
    56881 ctggcgcgcc acccttcaga aacccaggag cgtatgatgc tgcgcttgct ggcgtggctg
    56941 aaatatgctg atgaacgtct gcaatttacc cgtggtttgt gtgccgatga tgagccggaa
    57001 gcgtggctgc gtaacgatca cctgggcatt gatttgtgga ttgagctggg gctgccggat
    57061 gagcggcgga ttaagaaagc ctgcacccag gccgcagaag tggcgctgtt tacctataat
    57121 agtcgggcgg cgcaaatctg gtggcagcaa aatcagagca aatgtgtgca gtttgccaat
    57181 ctttccgtct ggtatctgga cgatgaacaa ctggcgaaag taagcgcctt tgccgatcgt
    57241 accatgacgc tgcaggcaac gattcaggat ggcgtgatct ggttatcgga cgataagaat
    57301 aatctggaag ttaacttaac cgcctggcaa caaccttcat gattgtgatt tcccgacatg
    57361 ttgctattcc cgatggtgag cttgagatca ccgccattcg tgcgcagggc gcgggcgggc
    57421 agcatgttaa taagacctca acggccattc atctgcgttt tgacattcgg gcgtccagcc
    57481 tgccagagta ttacaaagag cgtctactcg ccgccagcca tcatttgatc agcagtgatg
    57541 gcgtgattgt cattaaggca caggaatacc gcagtcagga actgaaccgc gaagccgcgc
    57601 tagcccggct ggtggcagtg attaaagatt taacaacaga acaaaaagcc cgacgaccca
    57661 cgcggcccac ccgtgcatcg aaagagcgca ggctggcatc gaaagcacaa aaatcaagcg
    57721 tgaaggcgat gcgcggcaaa gtacgcagcg gtcgggaata aaaagaagga atagatggtg
    57781 aaaaaagcga tagtgacagc gatggctgta atcagcctct ttactctgat gggatgtaat
    57841 aaccgggccg aagtcgatac gctttctcca gcacaggctg ccgaactgaa accgatgccg
    57901 caaagttggc gcggcgtgct gccgtgtgct gattgcgaag gaatcgaaac ctctctgttc
    57961 ctcgaaaaag acggaacatg ggtgatgaat gagcgttatc tcggtgctcg tgaagaacct
    58021 tcctccttcg cttcctacgg tacatgggcg cgaaccgctg acaagctggt attaaccgac
    58081 agcaaaggtg aaaagtcata ttatcgggcg aaaggcgatg cgctggagat gctcgatcgt
    58141 gaaggcaatc cgattgaatc gcagttcaac tatacgctgg aaccggcaca atctagttta
    58201 cctatgacgc cgatgaccct gcgaggcatg tatttttata tggctgatgc ggcgaccttc
    58261 actgattgcg cgaccggaaa acgtttcatg gtagcgaata acgcagagct ggagcgtagc
    58321 tacctggctg cgcgcggtca cagtgaaaaa ccggtgttac tgtcagtaga aggtcacttt
    58381 acgcttgagg ctaatccgga tactggtgcg ccaaccaaag tattagcgcc cgatacggcg
    58441 ggtaaatttt acccgaataa ggattgcagt agtttggggc agtaaacctg cttaaggcag
    58501 aaacaaacgc aa
//
LOCUS       DAZFWL020000016        57851 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFWL020000016 DAZFWL020000000
VERSION     DAZFWL020000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 57851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.016"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..869)
                     /gene="rfbA"
                     /locus_tag="ACHKNP_RS05380"
                     /old_locus_tag="pgaptmp_001076"
     CDS             complement(<1..869)
                     /gene="rfbA"
                     /locus_tag="ACHKNP_RS05380"
                     /old_locus_tag="pgaptmp_001076"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGU4703286.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGLRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNYVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMI"
     gene            complement(927..1826)
                     /gene="rfbD"
                     /locus_tag="ACHKNP_RS05385"
                     /old_locus_tag="pgaptmp_001077"
     CDS             complement(927..1826)
                     /gene="rfbD"
                     /locus_tag="ACHKNP_RS05385"
                     /old_locus_tag="pgaptmp_001077"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HGU4703287.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWLIHY
                     STDYVFPGNGDMPWRETDATAPLNVYGETKLAGEKALQEYCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTRHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQKFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(1826..2911)
                     /gene="rfbB"
                     /locus_tag="ACHKNP_RS05390"
                     /old_locus_tag="pgaptmp_001078"
     CDS             complement(1826..2911)
                     /gene="rfbB"
                     /locus_tag="ACHKNP_RS05390"
                     /old_locus_tag="pgaptmp_001078"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGU4703288.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEQSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVENVKSGAYQSWIAQNYEGRQ"
     gene            complement(3283..4176)
                     /gene="galF"
                     /locus_tag="ACHKNP_RS05395"
                     /old_locus_tag="pgaptmp_001079"
     CDS             complement(3283..4176)
                     /gene="galF"
                     /locus_tag="ACHKNP_RS05395"
                     /old_locus_tag="pgaptmp_001079"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HGU4703289.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGELSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSK"
     gene            complement(4351..5745)
                     /gene="wcaM"
                     /locus_tag="ACHKNP_RS05400"
                     /old_locus_tag="pgaptmp_001080"
     CDS             complement(4351..5745)
                     /gene="wcaM"
                     /locus_tag="ACHKNP_RS05400"
                     /old_locus_tag="pgaptmp_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HGU4703290.1"
                     /translation="MPFKKLSRRTFLTASSVLAFLHTPFARALPARQSVNINDYNPRD
                     WIASFKQAFSEGQTVVVPAGLVCLNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ACNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNVEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(5756..6976)
                     /gene="wcaL"
                     /locus_tag="ACHKNP_RS05405"
                     /old_locus_tag="pgaptmp_001081"
     CDS             complement(5756..6976)
                     /gene="wcaL"
                     /locus_tag="ACHKNP_RS05405"
                     /old_locus_tag="pgaptmp_001081"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HGU4703291.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQILRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(6973..8253)
                     /gene="wcaK"
                     /locus_tag="ACHKNP_RS05410"
                     /old_locus_tag="pgaptmp_001082"
     CDS             complement(6973..8253)
                     /gene="wcaK"
                     /locus_tag="ACHKNP_RS05410"
                     /old_locus_tag="pgaptmp_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HGU4703292.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDSGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKSLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTSKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTIGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQVMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVKSVLEHIREAK"
     gene            complement(8399..9877)
                     /gene="wzxC"
                     /locus_tag="ACHKNP_RS05415"
                     /old_locus_tag="pgaptmp_001083"
     CDS             complement(8399..9877)
                     /gene="wzxC"
                     /locus_tag="ACHKNP_RS05415"
                     /old_locus_tag="pgaptmp_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HGU4703293.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVACVAVFLLSDAI
                     GDMLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPALHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(9879..11273)
                     /gene="wcaJ"
                     /locus_tag="ACHKNP_RS05420"
                     /old_locus_tag="pgaptmp_001084"
     CDS             complement(9879..11273)
                     /gene="wcaJ"
                     /locus_tag="ACHKNP_RS05420"
                     /old_locus_tag="pgaptmp_001084"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HGU4703294.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(11409..12779)
                     /gene="cpsG"
                     /locus_tag="ACHKNP_RS05425"
                     /old_locus_tag="pgaptmp_001085"
                     /gene_synonym="manB"
     CDS             complement(11409..12779)
                     /gene="cpsG"
                     /locus_tag="ACHKNP_RS05425"
                     /old_locus_tag="pgaptmp_001085"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGU4703295.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(12972..14408)
                     /gene="cpsB"
                     /locus_tag="ACHKNP_RS05430"
                     /old_locus_tag="pgaptmp_001086"
     CDS             complement(12972..14408)
                     /gene="cpsB"
                     /locus_tag="ACHKNP_RS05430"
                     /old_locus_tag="pgaptmp_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HGU4703296.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPTGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(14411..15634)
                     /gene="wcaI"
                     /locus_tag="ACHKNP_RS05435"
                     /old_locus_tag="pgaptmp_001087"
     CDS             complement(14411..15634)
                     /gene="wcaI"
                     /locus_tag="ACHKNP_RS05435"
                     /old_locus_tag="pgaptmp_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HGU4703297.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPERIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGELCETFSGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(15631..16110)
                     /gene="gmm"
                     /locus_tag="ACHKNP_RS05440"
                     /old_locus_tag="pgaptmp_001088"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(15631..16110)
                     /gene="gmm"
                     /locus_tag="ACHKNP_RS05440"
                     /old_locus_tag="pgaptmp_001088"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HGU4703298.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(16113..17078)
                     /gene="fcl"
                     /locus_tag="ACHKNP_RS05445"
                     /old_locus_tag="pgaptmp_001089"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(16113..17078)
                     /gene="fcl"
                     /locus_tag="ACHKNP_RS05445"
                     /old_locus_tag="pgaptmp_001089"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HGU4703299.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVMRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(17081..18202)
                     /gene="gmd"
                     /locus_tag="ACHKNP_RS05450"
                     /old_locus_tag="pgaptmp_001090"
     CDS             complement(17081..18202)
                     /gene="gmd"
                     /locus_tag="ACHKNP_RS05450"
                     /old_locus_tag="pgaptmp_001090"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGU4703300.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(18228..18776)
                     /gene="wcaF"
                     /locus_tag="ACHKNP_RS05455"
                     /old_locus_tag="pgaptmp_001091"
     CDS             complement(18228..18776)
                     /gene="wcaF"
                     /locus_tag="ACHKNP_RS05455"
                     /old_locus_tag="pgaptmp_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HGU4703301.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(18792..19538)
                     /gene="wcaE"
                     /locus_tag="ACHKNP_RS05460"
                     /old_locus_tag="pgaptmp_001092"
     CDS             complement(18792..19538)
                     /gene="wcaE"
                     /locus_tag="ACHKNP_RS05460"
                     /old_locus_tag="pgaptmp_001092"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HGU4703302.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(19549..20766)
                     /gene="wcaD"
                     /locus_tag="ACHKNP_RS05465"
                     /old_locus_tag="pgaptmp_001093"
     CDS             complement(19549..20766)
                     /gene="wcaD"
                     /locus_tag="ACHKNP_RS05465"
                     /old_locus_tag="pgaptmp_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HGU4703303.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(20741..21958)
                     /gene="wcaC"
                     /locus_tag="ACHKNP_RS05470"
                     /old_locus_tag="pgaptmp_001094"
     CDS             complement(20741..21958)
                     /gene="wcaC"
                     /locus_tag="ACHKNP_RS05470"
                     /old_locus_tag="pgaptmp_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HGU4703304.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVVKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITCTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(21955..22443)
                     /gene="wcaB"
                     /locus_tag="ACHKNP_RS05475"
                     /old_locus_tag="pgaptmp_001095"
     CDS             complement(21955..22443)
                     /gene="wcaB"
                     /locus_tag="ACHKNP_RS05475"
                     /old_locus_tag="pgaptmp_001095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HGU4703305.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(22446..23285)
                     /gene="wcaA"
                     /locus_tag="ACHKNP_RS05480"
                     /old_locus_tag="pgaptmp_001096"
     CDS             complement(22446..23285)
                     /gene="wcaA"
                     /locus_tag="ACHKNP_RS05480"
                     /old_locus_tag="pgaptmp_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HGU4703306.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(23378..25540)
                     /gene="wzc"
                     /locus_tag="ACHKNP_RS05485"
                     /old_locus_tag="pgaptmp_001097"
     CDS             complement(23378..25540)
                     /gene="wzc"
                     /locus_tag="ACHKNP_RS05485"
                     /old_locus_tag="pgaptmp_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HGU4703307.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(25543..25986)
                     /gene="wzb"
                     /locus_tag="ACHKNP_RS05490"
                     /old_locus_tag="pgaptmp_001098"
     CDS             complement(25543..25986)
                     /gene="wzb"
                     /locus_tag="ACHKNP_RS05490"
                     /old_locus_tag="pgaptmp_001098"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HGU4703308.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(25992..27131)
                     /gene="wza"
                     /locus_tag="ACHKNP_RS05495"
                     /old_locus_tag="pgaptmp_001099"
     CDS             complement(25992..27131)
                     /gene="wza"
                     /locus_tag="ACHKNP_RS05495"
                     /old_locus_tag="pgaptmp_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HGU4703309.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            27790..29373
                     /gene="yegH"
                     /locus_tag="ACHKNP_RS05500"
                     /old_locus_tag="pgaptmp_001100"
     CDS             27790..29373
                     /gene="yegH"
                     /locus_tag="ACHKNP_RS05500"
                     /old_locus_tag="pgaptmp_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU4703310.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFCSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHGIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(29647..31500)
                     /gene="asmA"
                     /locus_tag="ACHKNP_RS05505"
                     /old_locus_tag="pgaptmp_001101"
     CDS             complement(29647..31500)
                     /gene="asmA"
                     /locus_tag="ACHKNP_RS05505"
                     /old_locus_tag="pgaptmp_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HGU4703311.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(31522..32103)
                     /gene="dcd"
                     /locus_tag="ACHKNP_RS05510"
                     /old_locus_tag="pgaptmp_001102"
     CDS             complement(31522..32103)
                     /gene="dcd"
                     /locus_tag="ACHKNP_RS05510"
                     /old_locus_tag="pgaptmp_001102"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HGU4703312.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(32195..32836)
                     /gene="udk"
                     /locus_tag="ACHKNP_RS05515"
                     /old_locus_tag="pgaptmp_001103"
     CDS             complement(32195..32836)
                     /gene="udk"
                     /locus_tag="ACHKNP_RS05515"
                     /old_locus_tag="pgaptmp_001103"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGU4703313.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            33154..36464
                     /gene="dgcE"
                     /locus_tag="ACHKNP_RS05520"
                     /old_locus_tag="pgaptmp_001104"
                     /pseudo
     CDS             33154..36464
                     /gene="dgcE"
                     /locus_tag="ACHKNP_RS05520"
                     /old_locus_tag="pgaptmp_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(36503..37360)
                     /gene="alkA"
                     /locus_tag="ACHKNP_RS05525"
                     /old_locus_tag="pgaptmp_001105"
     CDS             complement(36503..37360)
                     /gene="alkA"
                     /locus_tag="ACHKNP_RS05525"
                     /old_locus_tag="pgaptmp_001105"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HGU4703314.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            37494..38840
                     /gene="yegD"
                     /locus_tag="ACHKNP_RS05530"
                     /old_locus_tag="pgaptmp_001106"
     CDS             37494..38840
                     /gene="yegD"
                     /locus_tag="ACHKNP_RS05530"
                     /old_locus_tag="pgaptmp_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4703315.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQLPEAITQAVIGRPINFQG
                     LGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGGGT
                     TDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEKGI
                     ALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRERLSYR
                     LVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLALD
                     NAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR"
     gene            complement(38873..40792)
                     /gene="yegI"
                     /locus_tag="ACHKNP_RS05535"
                     /old_locus_tag="pgaptmp_001107"
     CDS             complement(38873..40792)
                     /gene="yegI"
                     /locus_tag="ACHKNP_RS05535"
                     /old_locus_tag="pgaptmp_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HGU4703316.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQA"
     gene            complement(40789..41550)
                     /gene="pphC"
                     /locus_tag="ACHKNP_RS05540"
                     /old_locus_tag="pgaptmp_001108"
     CDS             complement(40789..41550)
                     /gene="pphC"
                     /locus_tag="ACHKNP_RS05540"
                     /old_locus_tag="pgaptmp_001108"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HGU4703317.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(41547..42206)
                     /gene="yegL"
                     /locus_tag="ACHKNP_RS05545"
                     /old_locus_tag="pgaptmp_001109"
     CDS             complement(41547..42206)
                     /gene="yegL"
                     /locus_tag="ACHKNP_RS05545"
                     /old_locus_tag="pgaptmp_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU4703318.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(42427..42486)
                     /gene="ibsA"
                     /locus_tag="ACHKNP_RS05550"
                     /old_locus_tag="pgaptmp_001110"
     CDS             complement(42427..42486)
                     /gene="ibsA"
                     /locus_tag="ACHKNP_RS05550"
                     /old_locus_tag="pgaptmp_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HGU4703319.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(42759..42815)
                     /locus_tag="ACHKNP_RS05555"
                     /old_locus_tag="pgaptmp_001111"
     CDS             complement(42759..42815)
                     /locus_tag="ACHKNP_RS05555"
                     /old_locus_tag="pgaptmp_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4703320.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(43087..43143)
                     /locus_tag="ACHKNP_RS05560"
                     /old_locus_tag="pgaptmp_001112"
     CDS             complement(43087..43143)
                     /locus_tag="ACHKNP_RS05560"
                     /old_locus_tag="pgaptmp_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4703321.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            43422..44669
                     /gene="mdtA"
                     /locus_tag="ACHKNP_RS05565"
                     /old_locus_tag="pgaptmp_001113"
     CDS             43422..44669
                     /gene="mdtA"
                     /locus_tag="ACHKNP_RS05565"
                     /old_locus_tag="pgaptmp_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HGU4703322.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            44669..47791
                     /gene="mdtB"
                     /locus_tag="ACHKNP_RS05570"
                     /old_locus_tag="pgaptmp_001114"
     CDS             44669..47791
                     /gene="mdtB"
                     /locus_tag="ACHKNP_RS05570"
                     /old_locus_tag="pgaptmp_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HGU4703323.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVSTTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIASYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            47792..50869
                     /gene="mdtC"
                     /locus_tag="ACHKNP_RS05575"
                     /old_locus_tag="pgaptmp_001115"
     CDS             47792..50869
                     /gene="mdtC"
                     /locus_tag="ACHKNP_RS05575"
                     /old_locus_tag="pgaptmp_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HGU4703324.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIVISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            50870..52285
                     /gene="mdtD"
                     /locus_tag="ACHKNP_RS05580"
                     /old_locus_tag="pgaptmp_001116"
     CDS             50870..52285
                     /gene="mdtD"
                     /locus_tag="ACHKNP_RS05580"
                     /old_locus_tag="pgaptmp_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4703325.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            52282..53685
                     /gene="baeS"
                     /locus_tag="ACHKNP_RS05585"
                     /old_locus_tag="pgaptmp_001117"
     CDS             52282..53685
                     /gene="baeS"
                     /locus_tag="ACHKNP_RS05585"
                     /old_locus_tag="pgaptmp_001117"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HGU4703326.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            53682..54404
                     /gene="baeR"
                     /locus_tag="ACHKNP_RS05590"
                     /old_locus_tag="pgaptmp_001118"
     CDS             53682..54404
                     /gene="baeR"
                     /locus_tag="ACHKNP_RS05590"
                     /old_locus_tag="pgaptmp_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HGU4703327.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            54595..54927
                     /gene="yegP"
                     /locus_tag="ACHKNP_RS05595"
                     /old_locus_tag="pgaptmp_001119"
     CDS             54595..54927
                     /gene="yegP"
                     /locus_tag="ACHKNP_RS05595"
                     /old_locus_tag="pgaptmp_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HGU4703328.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            55136..55432
                     /locus_tag="ACHKNP_RS05600"
                     /old_locus_tag="pgaptmp_001120"
     CDS             55136..55432
                     /locus_tag="ACHKNP_RS05600"
                     /old_locus_tag="pgaptmp_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU4703329.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            55434..55730
                     /locus_tag="ACHKNP_RS05605"
                     /old_locus_tag="pgaptmp_001121"
     CDS             55434..55730
                     /locus_tag="ACHKNP_RS05605"
                     /old_locus_tag="pgaptmp_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4703330.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            55833..57194
                     /gene="yegQ"
                     /locus_tag="ACHKNP_RS05610"
                     /old_locus_tag="pgaptmp_001122"
     CDS             55833..57194
                     /gene="yegQ"
                     /locus_tag="ACHKNP_RS05610"
                     /old_locus_tag="pgaptmp_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HGU4703331.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(57524..57841)
                     /gene="yegR"
                     /locus_tag="ACHKNP_RS05615"
                     /old_locus_tag="pgaptmp_001123"
     CDS             complement(57524..57841)
                     /gene="yegR"
                     /locus_tag="ACHKNP_RS05615"
                     /old_locus_tag="pgaptmp_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HGU4703332.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT        13558 a        15459 c        14721 g        14113 t
ORIGIN      
        1 ttaatcattt tcagcagata ctgaccataa gcatttttct tcaacggttc agctaatact
       61 ttcacctgct cagcatcgat aaacccttta cggtaagcaa tttcttccgg gcaggaaact
      121 ttcagcccct gacgttcttc tattgttgcg ataaaattgc ttgcctcaat aagactttga
      181 tgtgtccccg tgtccagcca tgcataacca cgtcccatca tggcaacgga taaacgcccc
      241 tgttccatat aaatacggtt aatatcggta atttccagtt caccgcgggc agaaggctta
      301 agatttttcg ccatttcgac aacgtagtta tcataaaaat aaagcccggt taccgcataa
      361 ttactttttg gttgtagcgg tttttcttcc aggctgatcg ccgtaccgtt tttatcaaac
      421 tcaacgacac cgtagcgttc aggatcatta acgtgatagg caaataccgt tgcaccactt
      481 tctttgttaa cagcgacatc cattaacttc ggcagatcgt gaccgtaaaa gatattatca
      541 cctagaacca aagcacaatc atcaccaccg ataaactctt caccgatgat aaatgcctgc
      601 gcaagaccat ctggactcgg ttgcactttg tactgaagat ttagccccca ctggctcccg
      661 tcacccagca gttgttgaaa acgaggagta tcctgtggcg tactaataat cagaatatcg
      721 cgaagacccg ctaacatcag tgtagacaac gggtaataga tcatcggttt atcataaatc
      781 ggtaacaact gtttactgac agccatagtt acaggataaa gacgagtacc cgaaccacca
      841 gctaaaataa tacctttacg cgttttcatt tcatcattcc ttttaattca tcttgctcca
      901 ccatcacgaa caagatgcaa aaactattaa attgctgtag tcgtaaataa ttcgttgagc
      961 attcgtttca cgccaacctg ccaatcaggc aaaacaagcg caaatttctg ctgaaatttt
     1021 tctgtattaa ggcgagagtt atgcggacga cgagctggtg taggataggc tgttgttggt
     1081 actgcgttga gcttgttgag tgcaagggga atacctgctt tgcgtgcctc ttcaaaaacc
     1141 agcgcagcat aatcgtgcct ggttgtggta ccactggcta ccagatggta caagcctgcg
     1201 acttctggtt tattcagtgc gacacgaatg gcatgtgctg tacaatcagc cagcagttca
     1261 gcacctgttg gcgcaccaaa ctgatcatta ataaccgcta attcttcacg ctcttttgcc
     1321 agacgtaaca tcgttttggc gaagttattt ccttttcctg catagaccca gctggtccgg
     1381 aaaataagat gctttgcgca atattcctgt aacgcttttt ctccggctaa cttggtttca
     1441 ccgtaaacat ttagtggtgc ggttgcatcc gtctcccgcc atggcatatc gccatttcca
     1501 gggaagacgt aatcagtcga gtaatgaata agccaggcgc cgacttcatt ggctgctttc
     1561 gcaatcgctt cgacacttgt tgcgttaagt aattgtgcaa actccggttc tgattctgct
     1621 ttgtctactg cggtgtgagc ggctgcgttg acaataatat caggccgaat gcttcttacg
     1681 gtttcagcta caccttcagg attactaaaa tcaccgcaat aatcagtaga gtgaacatca
     1741 agagcaatca aattacccag aggtgccaga gcacgctgta gttcccaacc tacctgccct
     1801 gttttgccga aaaggaggat attcattact gacggccctc atagttctgt gcaatccatg
     1861 actgataggc accacttttc acattttcga cccattttgt attggacagg taccattcca
     1921 ctgtcttccg aatcccgctc tcaaacgttt cctgcggttt ccagcccaat tcgcggctaa
     1981 tcttctcagc atcaatcgca taacggcgat cgtgtcccgg acgatcggca acataagtaa
     2041 tttgctcacg ataagattgc tctttcggta caatctcatc caacaaatca caaatagtca
     2101 gcacaacatc gatgtttttc ttttcgttgt gtccgccaat gttataagtt tcacccgctt
     2161 taccttcggt gacaacggta tataacgcac gcgcatgatc ttcaacatat agccaatcgc
     2221 gaatttgatc ccctttgcca taaataggta atgccttacc ttccagagca ttaagaatta
     2281 ccagcggaat caatttttcc ggaaagtgat aagggccgta gttattcgaa cagttagtca
     2341 caatggtcgg taaaccatag gtacgtttcc acgcgcggac taaatgatcg ctagatgctt
     2401 tggatgcgga ataaggactg cttggcgcgt aagctgtcgt ctcagtaaat aagggtaatt
     2461 cttctgtatt atttacttcg tcaggatgag gcaaatcacc atagacttcg tcagtagaaa
     2521 tatgatgaaa acggaagcta tttttcttgt cgctatcaag agcagaccag taattgcgag
     2581 cggcttccaa aaggacataa gtaccaacaa tattggtttc aataaatgcc gcagggccgg
     2641 taattgaacg gtcaacatgg ctttcagcag ccaggtgcat cactgcatcc ggctgatgct
     2701 gagcaaaaat ccgcgccatt gcagctgcat cgcaaatatc cgcatgttca aaaacatagc
     2761 gttcagaatc agaaacatca gcaagtgatt ccaggtttcc ggcgtacgtt aatttatcga
     2821 cattaacaac actatcctgc gtattattta taatgtgacg aactacagca gaaccaataa
     2881 atcctgcgcc accagtaaca agtatcttca ctttattatt ccatattact tcagagcatg
     2941 ctgtgaaata agcggctctt aggttgatta ataggggtat taatgcacgc taccgcccct
     3001 ggctttacag ctaccagtgc actgcatgca tgtctacgat gtgacgagcg ttacccactc
     3061 gcgctaaacc cgaaaaattc aaacgctaat tgtcttacca atccgctctg gaaacaagga
     3121 aaatcctgga aaactttgac taaaatccta ttgctaactc gttgttattc tgattgttta
     3181 tataaaacaa cggcaggaat attcgcaaca aattactttc atcacgaatc ttcactgccg
     3241 ttataatttt cttatcaacc gttacatccg gtcagatttt cattatttgc ttaacagctt
     3301 ctcaatgcct ttacggaact tcgccccttc tttcaggttt cgtaatccat acttcacgaa
     3361 cgcctgcata tagcccattt ttttaccgca gtcgtaactg tccccagtca tcagcatggc
     3421 atcaacggac tgttttttcg ccagctcggc aatagcatca gtcagttgaa tacgtcccca
     3481 tgcaccaggc tgagtacgtt ccagttccgg ccaaatatcg gcagaaagta catagcgacc
     3541 aacggccatg atgtctgagt ccagcgtttg tggctgatcc ggtttttcga taaattcaac
     3601 aatgcggctg actttacctt cgcgatccag cggttctttg gtctggatga cagagtattc
     3661 agagagctca cccggcatac gttttgccag cacctggcta cggcccgttt cattgaaacg
     3721 cgcaatcatg gcagcaaggt tgtagcgcag cgggtcggca ctggcgtcgt cgatcacaac
     3781 gtccggcagt accacgacaa atggattgtc accaatggcg ggtcgtgcac acaaaatgga
     3841 gtggcctaaa cctaaaggtt cgccctgacg cacgttcata atggtcacgc ctggcggaca
     3901 gatggactgt acttccgcca gcagttgacg cttcacgcgc agttcaagaa gagattctaa
     3961 ttcataagag gtgtcgaagt ggttttcgac cgcgttcttg gacgcatgag ttaccaggag
     4021 gatttctttg atccctgcag ccacaatctc gtcaacaatg tactgaatca ttggcttgtc
     4081 gacgatcggt agcatctctt tgggaatcgc cttagtggca ggcaacatat gcatcccaag
     4141 acccgctaca ggaataactg cttttaaatt cgtcattatt tcatccacct gtaaaatggt
     4201 tgctgaatta tagcttgttc gatttttttc gccagcatca attaccctga attgattact
     4261 gaattacttg tgatgttacg ccgcttcgtt gtagattgca gtagcattgt tcctaagtat
     4321 ggctccattt ttccaggaat ggtcgcaaat ctactccctc cgttccggca atctaaagtt
     4381 aatcttttcc acattaacaa tatggtgatt tatcctgtcg atatcgacgg agctttgccc
     4441 tctttcattc accgcatgaa catttgcaag agatagcagt gtttcttttt tcgccataaa
     4501 cacgccgcga acgtctttgc gcatgtcgaa gttcatgctc aatgcgggtc caacagagga
     4561 ttcctgcatc actttgatat tacgcataaa aaggtgttgc ggcttgttgt gtaactccag
     4621 cgacgcacgc ttcatctcaa cgttagtcag cgccacaaag gagacagcat tcccggcgga
     4681 gatttggatg ccgcgcaatt tgtaagcaag gtgggtatta tccagctgaa tattattcac
     4741 tcggaaattc tgcggtatcg agagatattt gcctttaatt accccatagc cgattaacat
     4801 cccggcgcta ttaatcattt caatattatc aatcacgaaa ttgtcacaac cgtaaatagc
     4861 gaccgtggcg ttatcaatac ctgctttctt actgaaatcc ggcgtgatat tgcaggcttt
     4921 gatattacga ataacaaaat gtttaccatt ttcaacatgt atcaactgcc gacaatccga
     4981 tcccgtgatg ttcgccacga caaagttttt cactgcctgg tcttccggat agttgttatc
     5041 ataagtgctt cccgcaagac ctatgccgat gccccaattg attttgccgt tagtacagtt
     5101 gatgcgctcg atgacatggt cagatatcaa aatatcacgg tcgttaatcg ccacattcca
     5161 ttcaatggcg tcgccctgta agtcgctgaa cttacaattg gtgatgttgg caccgataat
     5221 ctggttatga aatccctggc gtaagatggc gtaattagcg tggctaacgg tcaggttatc
     5281 gatggtcagg ttacgcatga cccgtttgtt tttgccgccg atataaatct gcgttaccgg
     5341 accaaagccg ctcatcgcca gccctttgat ggtgcagtca gagccgcgca catccagggt
     5401 gatgttatgc atactgccgc cctcctcccc tgtcacctgg ctgccgtcct gtaagacaaa
     5461 tcgccctctg ccgttgccgc gcaggcttcc aaggatgtgt aacgttttgc caggagggat
     5521 aaagatgccg gtgttgatat tgaggcaaac caatccggca ggcacgacga ccgtttgccc
     5581 ttcgctgaag gcttgtttaa atgaggcgat ccagtcgcga gggttgtagt cgttaatgtt
     5641 aacgctttgt cgggcgggaa gcgcgcgagc gaaaggggta tggaggaagg caagcaccga
     5701 gcttgccgtc aggaaggtgc gtcgggagag ttttttaaat ggcatgcgtt ctcctctata
     5761 aagcctgcag caagctggcg agttctcgat tgatcacctg ctggttaaaa tcgtgttcga
     5821 ctttttcgcg cgcacgtttg acgaccggag ccagttcgtc ggtgtccagt tggctaaacg
     5881 ccgccagtcg ttgcgccagt gcgcgagcat cgttctcagg caccagccag ccggatttat
     5941 cggcctccac cagttccggt attccgctat gcagagtaga aaccaccgga atgccgaccg
     6001 ccatcgcttc cattagtgcc accggaatac cttccatatc gccatccgca cctgtaaccg
     6061 atggcaacag gaagacatcc gcgtcgtcga gcatcgcttt cacttcgtgg ctcggtttaa
     6121 agcccggcat ctctaccaca tcttccagtt gatattgttc gatgagggtg cgcaggcgtc
     6181 gttcccacgg gccaatgccg aggatgcgat agcgaaatgc cacgccctgc tctttcaact
     6241 gacggcaggc ttcaatcgcc acatgcaggc cttttttctc ggttaagcgt gcgacggaaa
     6301 tgatttccag cggcgttgcg ggcgctttca ccggacgcgg gctaaaacgc gtcatgtcca
     6361 cgcccatgcg cgatacggcg attttttccc tcgggcagcc cattttttgc aaccttccgg
     6421 cccacagatc gcttattggt agcatcagat cgccacggcg gaacagttgc tgatattcgg
     6481 gagtgtaatg gttgagcacc tcccgactgg agatatcaat gccgtggaag atagtggcaa
     6541 ttttgccgcg aatgacaccc agttcgcgga gttttgctgc ggttaccccc gcagggccaa
     6601 aatgagcgat aaagacatcg gcataaaatg gtgttgcgac ctggccgcaa atggcagaca
     6661 aaatcaggtt tcgcgactcg gcaccatagc gtttgaggtt aagtgcctgc caggtatttt
     6721 tacgatgaat gccgcgtaag atctggctgg cacggtggcg cagtttcgcc actttgcccg
     6781 taggttcgtc ctgtaaccaa cgggttctgg cggcaaggtt gtatttcgtc catgccgcgt
     6841 gggtgttctg ggtgtcgcct ttttgcagcg cgacaatctc cacctcaaag cccatatcaa
     6901 taaacgcggt aatttgattg aggacgaagg tttccgacga cagcggaaat ttcagtaaaa
     6961 agaagccgac cttcatttcg cctccctgat gtgttcaagc acagatttca ccatctgcat
     7021 tcccgtctga cgttcgcgac tgacggcttc gttaagtcgc gtgttcagtg ccggaagctg
     7081 gcctaaggta tccgcaacca tcacttgcag gctgccgtct aataaatgac ggatatcaat
     7141 cgccatctcc ggtagtccca gctgctgcat aatcccggcg gatttatgtt cataattgat
     7201 ggcaatcgcc ggggtggcaa agttcattga gataatggcg gagtgcaggc gcgtaccgat
     7261 ggtgagttca caggccccca gaattttgcc catttccaga tcgttgagtt catccatcac
     7321 tacgtggtaa cgggcaggat cgctgatgtg ctggcgcagg ttgagcgcca ccatgcggtc
     7381 gtctttgtta tagctgtcga tacccgtaca ggtggagagc gcaatcacct gatagccttc
     7441 gtcgagaatg cgattgacca ccccggcaaa ggctttttca tatgcttgtt gagtggtgcc
     7501 gagacgtttg tcgaacggtg ccagttcgcg cagggtaatt gccaccgttt tctgttgtgc
     7561 ggcaacgtcc agccagtgtt gaacagcata gctggcggtg aagtcttctg tgtggtgatc
     7621 gaccagccac gcggtatcga cgccatgttc aacttttgaa gtggtgatat tgctgcgttt
     7681 catcagttca aggctgaccg attcgcgcag gatcagcgcg tcgcagtgac caaaaacgta
     7741 gttcgccagt tggttaaatt gctcatcctg gaacgggcca acactgtgac caatcataaa
     7801 cagcgacttt ttcgccataa acgtgcaaag tgcatgttca aactgtggca cgccgtagag
     7861 atcgacaaaa aacgatccgc cgacctgaat aatggcgtcg taacctgaca gcaggcgcac
     7921 gaagtcggtg aatccctggg cgatggcgat attacgcagc ttgccactgt cggtgacgcg
     7981 tgagagcaat acctgatgct ggtagcggcg acggaggact tttttaacgc gcccgacaac
     8041 gcccgccgcg ctgttgtgtt gtttcatttg caggaacagc ggatcgccca ttaccgggcg
     8101 gttgagtagc caggaagaac tgacaggata gcggctcatc acgtccactt cggcatgtgg
     8161 attgaggatg ttgatggcat caagtaagcc gcgcaagatg gcgctgtcgc cacgattgcc
     8221 gcaagtgtgg ttgcccagaa taagtaattt cattttttcc tcataaattt gatgccaggt
     8281 gaggctgtgt ttgttgtgtt cgttgccgtt ttttgcctga tgcgagcaca gaaccgtagg
     8341 acggataagg cgttttacgc tgcacccggc aaccgttgtc ggaaccgaaa acaacaactc
     8401 accccgcccg taaaagcatt ttcatttttt cgctgcgaca aaactgacgc ttcacttcca
     8461 ccaccagcgg atggcgcgac agcacaatca tcaccacaaa cgccagcaca cccgcggcta
     8521 tttgcaccgc cagcagcatc cccagcgcca gttgcccttt cagcacaatg cccagcgcat
     8581 aactgaccac cagcgttggc agcgagagat aaaacggcag ccacaaactc aggatgtact
     8641 ggcgataact ggaaccaaga accggtttaa tcatcacgaa atagctcaga atagtgttga
     8701 taatctgcac cagcaggaag ccaagcgtga cgccgattgc gcccgccatc tgcccaccaa
     8761 taacaatcgc cggaataaac agaaatgttt taaatacgtt gaatttaaag ctgatatcga
     8821 cccgcgcttt cgccatcagc agcgaaccaa tcgggttacc cacggagcgc agcagaccca
     8881 ccacacacag cagctgtagc accggaataa tgctgctcca cttttcgcca aataccagcg
     8941 gtacaaagtt attcgacacc accatcagcc ccagcagcgc cggaaagttg ataatcccca
     9001 ctaccgacag cagcttgtag aagttgacgc gcagcttttc ggtatcgtcc tgaattttgg
     9061 caaacgccgg aaacaacacg cgggtgatga tcgggttcag tttcatcggc ggcacaacgg
     9121 ccacgttgta cgccaggttg tatcccccag ccacgcccgc gccgagaata cgcgccagca
     9181 cgagtgttga aaggttggtg ttgagatagt tgatgatgct gtccgccgtc agccaggcac
     9241 caaagcgtaa gttcggtgcc accgacgcca gcgagaaatg cagagcgggg cgatagattt
     9301 tgcggccaaa gtagccaaac agcagcgttc tcaccgcact attgaccaga taaccgagga
     9361 tcgcggtcat cgccagcggc cagaaatggg cgctaaccac cgtaaaagta aagcccgcca
     9421 gcaccgcgct ggtttcgatc atgccgattt tgttgaactc cagctctttt tgcatcaacg
     9481 cgcggaactg ttgcccgtgg gggatcacca caaacgctag tgacaatgtt ttaatcaacg
     9541 gtgccaggtc cgggttattc agcatgtcgc caatggcatc actcaacaaa aataccgcca
     9601 cacacgccac gatccccagc ccgacgttca gccagtacaa cgtggtgagt tcaaggtggc
     9661 tgatttcttt tcgctgaata atggagttag cgataccgaa gtcagaaagc gtatctgcca
     9721 gcgcgataat caccagcgac acagtcagca aaccgaactg gtggttgtcg ataatccgcg
     9781 ccagcacagt catttgcacc agcccgaggc cgatgatgat caccgtggca atcgccgacc
     9841 acttcgcgcc gctgatggtt ttttcacgta agctcatatc aatatgccgc tttgttaacg
     9901 aagcctttga ataccgtcag gaaaacgatt ttgatatcga accagacgct ccattcgcgg
     9961 atgtactcaa ggtcgaactc gacgcgtttt tccattttct ccagcgtgtc ggtttcgccg
    10021 cgccagccat taatctgcgc ccagccggta atgcccggtt tcactttgtg gcgcagcatg
    10081 tagccttcaa tgagctggcg atactgttcg ttatgcgcta ctgcgtgcgg acgtggaccg
    10141 acaatcgaca tccccccggt cagcacattg ataaactgcg gcaattcgtc cagcgaggtg
    10201 cggcgcagaa agttccccac tttggtgacg cgcggatcgt tctgcgtcgc ctgagtcacc
    10261 actttgtcgt tctccatcac tttcatcgaa cggaacttcc acactttgat cggcttgccg
    10321 tccatgccgt agcgagtctg gcggaaaata accggccccg gtgagctcag tttcaccgcc
    10381 aacgcaatgc agcacagcac cggggagatc agcagcagaa tcagcgttgc cagcacaatg
    10441 tcttctgcac gtttgagcag gcggttaacc ccggaaagcg gcgtgtcata cagcggcacc
    10501 actggtacgc cgttcatctc ttcaatacgg gaatggagaa tgttgaaggt aaagacatcg
    10561 gggatcagca gcaccgaaca ggtagtgtcc gccagttgat ggaccagttt tttcactcgc
    10621 gcgccgtcgc acatttgcat cgcgatatag acgttatgaa tcttgcccgc tttcgcatcc
    10681 tcgaccagct gttgcagatt gcccgcccag tcgttagaaa cgccacccgg tttcgggtcg
    10741 tggtaaacgc ccaccacttc aaaccctaac cacggctgat tacggaagct ctccatcagc
    10801 atttgtccgg cggctaaatc ccccgctacg gcgaccatac gcttgttata gccatgatta
    10861 cgcagccagc ccgcgccaat gcgaatacat gaacggcaaa ccaccagtcc gatgctggtc
    10921 agcccatacc acgccagcca gattttcagt tgcgtgtcga aatcattatt gaacgccacc
    10981 agtccggcgc tgaaaatcac gcttaaggtc cagttttgta gcaacagggc aaattctgtc
    11041 gctgcccgaa caccgcgcca tgagcgataa aaatcggtga tgccgcccag catctggaac
    11101 accaccagcg taatcaacgc caccaacagg tgcatgtaga ggaatgacag tccgctcact
    11161 tcgcaaacca gccatagtcc ggcaaacatg atggtgatat ctgaaaagcg ttgcaccata
    11221 gagattaacg atgcattggt tttcgctcgc tcgcgctttt ttagatttgt catcgttgtt
    11281 cctgttctat tgtcgcctcg cccctcaccc cggccctctc cccaaagggg cgagggggag
    11341 aagaaagttc cgtgctcact tctctccgca ggcagtagag cgtcagggtg agggggaaag
    11401 taatgacatt actcgttcag caacgtcagc agagttcgcg ttcgcgcttc catcagcggt
    11461 acatcaccgc gcgattccac attcaaccgc accaccggtt cggtgttgga ggagcgcaga
    11521 ttaaagcgcc agtcggcaaa ggtcatgctg atgccatcgg tgcgatccac cgccagcgcc
    11581 tcacggctaa agtgctgttc cacgcggtta atcgcctcaa cgggttgcgc cagtttgctg
    11641 ttgatctcac cgcttgccgg aaacgccgcc atccggtcgc gcaccagttc gcccagcgtt
    11701 tttcctttca ggcacaccag ttcggcgacc agcagccatg ggatcatgcc gctgtcgcag
    11761 taagcgaaat cacggaagta atggtgggcg ctcatttcgc caccgtagat ggcgtcttcc
    11821 ttgcgcatac gttctttaat aaaggcgtgt ccggttttcg acattaccgg ggtgccgcct
    11881 gcggcagtca ccacatcaac ggtattccag gagagacgag gatcgtggat gatcttcgcg
    11941 ccgggatttt tttcgaggaa cgcttctgcc agcaggccga caatgtagta accctcaata
    12001 aactgccctt tttcgtcaaa caggaagcag cggtcaaaat cgccatcaaa ggcaatgccc
    12061 atatccgcgc cgtgtttgat gaccgcattg cgggtgtcgt cgcggcattc cggcagtagt
    12121 gggttaggaa taccgttggg gaaattgccg tccggcgtgt tgtgcacttt gattaattcc
    12181 acgggtgcgc cgagggcttt aaagcgggct tcaatggcgt ccaccaccgg acccgctgcg
    12241 ccgttcccgg agttgatcac cagcttgagc ggcgtgaggt ttttgacgtt gatataaccg
    12301 aacaggtgat caacgtaagc gtcacgcagg ttgatttgct gatagcgacc gcgtttggtt
    12361 tcatcgacgg gagggaagtc gttggcttca gccagacgct ggacgtcgcg cagtccggta
    12421 tctccgctga tcgggcgagc cccctcgcga accagcttca tgccgttata atccatcgga
    12481 ttatggctgg cggtaacttc aatgccgcca tccacgccga gatggaacgt ggcgaaatag
    12541 atctcttcgg tgccggacat gccaatatcc agcacatcga cgcccgcatc ctgtaaacct
    12601 ttcgccagcg ccagttttaa ggtttcgctg gtgaggcgga catcaccgcc taacacaatg
    12661 gttttcggtt tgagaaattc gccataggcg cgaccaatgc gccaggcaat atcttcattc
    12721 agttcttcgc ctaatttccc gcgaatatca taggctttaa agcaggttaa ttttttcata
    12781 tcgttaccct ttttcaggca atagttggcc ctgaccgaaa cggccaattt tgtttttgtc
    12841 gttatttaca tcgcggtttc gcattcattg cctgatgcga cgctgccgcg tcttatcagg
    12901 cctacaaatc cgaaccgtag gtcggataag gcgttcacgc cgcatccgac aaacagcgcc
    12961 tgatgcgacg tttacacccg tccgtagcga tccgcgaagc gcaccacatc atcctcttcg
    13021 agataagagc cggagcgcac ttcaattaaa tcgagcggaa ttttccccgg gttttccagg
    13081 cagtgcgtcg cccccagcgg aatataaatg gactcgtttt caccaagcag tttgatatca
    13141 ccgtcaatag tgacttttgc cgttcccgcg accaccaccc agtgttccgc gcggtgatgg
    13201 tgcatctgta ccgacaagcc ttcgcccggt ttcacggtga tgcgtttcac ctggtagcgg
    13261 tcgcccgcgt cgatagagtc atatttgccc cacggacgat acacttcgcg atgtacccga
    13321 tgctcatggc gaccatcggc tttgatctgc tcgaccactt ttttcacatc ctgcaccgcg
    13381 ttacggtcgg caatcagcac cgcatctttg gtctgcacta ccaccagatc tttcaccccg
    13441 acggtggtta cgaggccaga ttcggcatac acatagctgt tttctgtttt gtgattaatc
    13501 acatcgccgt ggcaaacgtt gccctcggcg gtgtgggcgc tgatctccca taatgaagac
    13561 caggaaccga catcgctcca gcccgcatcc atcggcacca caacggcatc tgccgtacgt
    13621 tccatgaccg cgtaatccac cgactcttcc gggcaggcga gaaacgcttc ttcatccacg
    13681 cgaataaaat cgagatccgg atcgacggcg ctcatcgctt tttcgcaggc atcgaggata
    13741 tccgggcgat attttttcag ttcttcgaga tagcgtccgg cgcggaacag gaacatgccg
    13801 ctgttccagt aatattcgcc gcttgccaca taggcctggg cggtttccag attcggtttt
    13861 tcgacaaact gcgccacttc aaaggccacc gtatcctgct cacccgcaga cacttcaccg
    13921 cgacgaatat agccataacc ggtttcaggt agatccggca caatgccgaa ggtcaccagc
    13981 ttgcccgctt cggcatatgg catggcatta cgcacggcgg cacggaacgc gtcttcatcg
    14041 gcaatcacat gatccgccgc caataccagc attaacgggt cgctctccgg gctatgacgt
    14101 tttgccgcca gcgccgccag tgcaatggca ggtgcagtgt tacgccctgt cggttcgaga
    14161 ataatgttct cggtgagttt gttcagttga cgcagctgtt ccgcgacaat aaagcggtgc
    14221 tgctcattgc aaatcaccac cgggctttcg cactccacgc cgttcaggcg gcagatggtg
    14281 gtttgcagca tggtgagatc gcctttcagg cataaaaact gcttggggta aagtacgcgg
    14341 gaaagcggcc ataagcggct accggagcca cctgccatca caactggata gagtttcgac
    14401 tgcgccataa ttatccccga atatcattta taaattgacg taacacgttc tctttatcga
    14461 gcgtgcgttc ggcatattca cgtgccaccg tgttgtgttt gggcagcagg agcgcctgac
    14521 ggatccccgc caccagcgcc tcgaccgatt ccggttctac gcaaaccgca atgcccgaaa
    14581 aggtttcgca aagctccccc agttctgtgt gggcctctgc ggtaatcacc gcgttaccgc
    14641 ctaccgccag aatattggtc agtttcgacg gcaatacggc atctgccgcg ccgcgttttt
    14701 gcaccaccag atggcaatcg cccatcttca gcagtgcggg taaagcgtca tacgattgca
    14761 gcgggaaaaa ttgcatgttg tgcagtccac gctgctgcgc cattttttcc agtcgcgctt
    14821 tgccgccacc ctgcccgaca atggcaaaaa tcagtggttc atcgcgcagg cgatcggcag
    14881 cttcaataac gttttccagc ccctgctttt caccaatatt gccggagtaa agaatgattt
    14941 ttttgttatc cggcaggcca agctggttac gaagggcatc aacatcggca tctgcaacat
    15001 gctgaaaacg ggcgatttcc gaccagttgg ggaagaagat gacgttttcc gccgccacgc
    15061 ctttttcgat ggctttattc atcatcgaac gcgaaatcgt ggagacgtta tcgacgttat
    15121 gtagtccgct acgttcgaac gccgttgcca gctgtgccac tttgccgcct ttgccttttc
    15181 cggcaaggcc cagccccagc atggcatcta cttcgtaatc ctgaatatgc agcacggtac
    15241 gcgcgccaga gagtttcgcc agcagacgca ttcccggcgt gcaaaacagc gttggcacta
    15301 cgccgataat gcgctccggc ttccagcgac gttgcgccat cagcgggaaa aaactgctga
    15361 cggcaaaact gccgagatgc aacaggcgtt tcagggtgct cggctgtttt ggcacataca
    15421 gcgggcagcg ccacaccgtg gcggccccct cttctcgttt gtagcgccag gcggaatagt
    15481 tctcgcccac ctgccactgc gggtagtaag gcggtgcggt aatgacccgc acctcatgac
    15541 cttgtgccgc cagccattcc accatctcgc cggtgtattt gccgatgccg gttaactccg
    15601 gcgagtagtt aatgccgtag accagtattt tcataatccg ggtactccgg cacgcttctc
    15661 agcgagaaaa taggcgcggc tgttagcgtg aacattgtca ctggcgagca gcgcgtccgg
    15721 cgtcagccag cggtaatcgt catgctgctc atccggcagt aacagatctt cttcagcgac
    15781 tctgaagcga aaaccgagca ccacatagtg agtggtgaaa tccgtgccgg agaagttatc
    15841 gtcataaaag tgctgccaga caccgtaaaa ctggcctgct gttatcggca gacgcagccc
    15901 cagttccgcc atcgtcagcc gctcaaatgc ggcttccagc gtttcgtctt tctgcacgcg
    15961 ccctcccggc acaaaccagt aaccctgcgc cgggcggttg gttcttttgc caagcagaaa
    16021 ctcgccgcga ctgttctcga caataaagtc gagagagaca agcggtgtgg agcgcactac
    16081 cgtggcaaag tcttcctgac gtaaaaacat cattaccccc gaaagcggtc ttgattctca
    16141 aggaaccact ggtaagtgct ggcaagcccc gcttccagtg atatttcgtg ataccagcca
    16201 agctgatgca ggcgcatcac atccagcagt ttgcgcggtg tgccatccgg tttgctggca
    16261 tcaaaaacca cccgaccttt gtaacccacc actttggcga tggtttgcgc cagctcgcgg
    16321 atagtgcagt caacgcccgt gccgacgtta atgtgcgaca gcatcggctg ggtgttctcc
    16381 agccagactt cgtgcgccag ctccatcaca tgaatgcttg ccgccgccat atcatcgacg
    16441 tgcaggaatt cacgcatcgg tgtaccgctg ccccacacca ccacgtccgg cgcattctgt
    16501 gccgtcgcct cgtggaagcg acgcagcaat gcagggatca catgcgaatt actcgggtgg
    16561 aagttgtcgt gtggcccgta caggttggtc ggcatgactg aacggtaatc tcgtccgtac
    16621 tggcgattgt aagattcgca caatttgatc ccggcgattt tggcaatagc ataaggctcg
    16681 ttagtcggct ccagcgtgcc ctgcaacaac tcgctttctg ccatcggctg ttttgccagt
    16741 ttcgggtaga tgcaggacga tccgagaaac agcagtttgt tcacgtcgtt ctgatgcgcg
    16801 gcgtgaatga tgttgctctc aatcatcatg ttctggtaga tgaaatccgc cggataggtg
    16861 ttgttggcaa caatgccgcc cactttcgcc gccgccagat agacctggtc aatacgttcg
    16921 ctggcaaaga aatcatgcac ggcgcggctg tccagcaggt tcagctcgtc gcgggtgcgt
    16981 aataccagtt ccacatcacc gcgctgttcg agctgtcgct tgatggcgga accgaccatc
    17041 ccgcgatggc cagcgataaa aattcgttgt ttactcatgc ttatgactcc agcgcgatcg
    17101 ccacgtcgta gccgtgagat ttcagcagag agtgtttttt cgccgcttcg aggtcattag
    17161 ccaccatttc agacaccatc tctctgaggg tgatttccgg tttccagccc agtttttcgt
    17221 gcgctttggt cgggtcgccg agcagcgttt caacttccgc cggacggaag taacgcgggt
    17281 caacggcgat aatcacatcg cccggtttaa cgcccggcgc gtcatgcccg gtgacggaaa
    17341 ccacaatgcc cttctcttca acgcccgtgc cttcaaagcg cagtttgatg cccagctgtg
    17401 ctgccgccat ttccacgaac tgacgtacgg agtactgaac gccggtcgcg ataacgaaat
    17461 cttctggctg ttcctgttgc agcatcatcc actgcatttt tacgtagtct ttggcgtggc
    17521 cccagtcacg cagggaatcc atattgccga ggtacaggca cgactccagc ccctgggcga
    17581 tgttggcgat tgcgcgggtg attttgcggg taacgaaggt ttcgccgcgg cgcggggatt
    17641 catggttgaa gagaataccg ttacaggcgt acatgccgta ggattcgcgg tagttaacgg
    17701 tgatccagta ggcgtacagt ttggcgaccg catacggaga tcgcgggtag aacggcgtgg
    17761 tttctttctg cggaatttcc tgcaccagac cgtacagttc agaggtggaa gcctgataga
    17821 aacgagtttt cttttccaga ccgaggaagc ggatcgcctc cagcaggcgc agcgtaccca
    17881 tcgcgtcgac gtcagcggta tattctggtg actcaaaaga gaccgcaacg tggctcattg
    17941 cgcccaggtt gtacacttca tccggctgta cttcacgcaa aatgcgcgtc aggttagagg
    18001 tatcactcag gtcgccataa tgcagatgga atttcgggtt gcaggtgtgc ggatcctgat
    18061 aaatgtgatc cacgcgctcg gtgttgaatg acgatgcgcg acgcttaata ccatgcacct
    18121 cgtaaccttt ttccagcaga aactctgcca ggtaagaacc gtcttgtccg gttacaccgg
    18181 tgatgagagc gacttttgac atgtcttatt cctctgtatt tttgaattta ttcagtttca
    18241 acgcgttcgc gtatcaccac tgcgggattc ccccggcaaa ccacatttgc cggaagcgat
    18301 ttaaaaacac tgcttcgtgc acccacgacg gtgccgtcgc cgattgtgac gcctggggca
    18361 acaaagacat cggttgccag ccagcatttc tcgccaatca caataggcgt ggcgttaatg
    18421 gtgaaatgtt gacttgcatg gtcgtggcta ccggtgcata aataactttt ttgcgatatc
    18481 accgaatgtg cgccaatggt tatttcaccg agggtatata aattgacgtc atcgccgacc
    18541 cacgcgtaat cacctaaggt taatttccac ggataggtaa tttttactga cggacgaata
    18601 actacgtttt ttcctatttt tgctccgaat aaacgtaata aaaaagcccg ccagcgatac
    18661 aatacttgtg gcgaccaggc aaatattgtt gcctgtactg cccaccataa ttgcacttta
    18721 atagcgttgc cgccccggaa ccctttcggc accgagaaac cgcttaaatc ttgcattggt
    18781 tctccttata ttcagacttt gttatataag gctttcgtct ttgaggtagt acgttggcgt
    18841 aaatgccagg ataattcagc ccaaaagcca ggtacatgta atatttgtcg ttggactttt
    18901 ttcgcgtcag cacacaattc catattattg gtggtagata ccccacccat ggaaaattca
    18961 gacaccaggc cattgagttt tttaaatgca taaccggctt tatacatttt ggctgccagc
    19021 gcgtagtcgg aagaaacttt atattccagg tcataacgcc attttttcaa gccggataca
    19081 gggaaaaata tcgcctgatg actggcgggc aggctgtgat aaatatacca gcccggtttg
    19141 gcgctacgtt taattttatg cccgtcgcca aaatccagca gcgcatcgcc ggtgatcatc
    19201 atgttatctt tttgcatttt taacttacgg acaaaatttg cggcatcctg atgaaaaata
    19261 tcgcccgaat tgagaaacaa cgcgaacttg ccttgtgcca tcgtaatgcc tttgttcatg
    19321 gcgtcgtaga taccgttatc tggctcgctg acaaagcgta ggttatagat accattgaga
    19381 ttttccagat actcacgggt gccgtcattg gaaccgccat cgacaacaat ccattcgaag
    19441 ctgatatctt ccgcctgcgc cagatgcgcc agcgaggcat gtgttttgac tatcccttcg
    19501 aggttacgaa acgcgacagt gattatgcta agcaacatgt tcttattctt acctcgtaat
    19561 atttaacgct tttcgcaaaa taaacggaca gacgattaaa aatgcatatt ccgggctaaa
    19621 tatcgaaccg gtaaaaaaca gcgacaccgg agtaaaaaga tataattgca cgcgaaaatt
    19681 gcggttatca ccaaaagcgt tgatcatcat ttttatcact ttccccatgt accacagcga
    19741 taaaaacact gcgaaccagg aaaaataaat aatcagcaga tacaaaccat tgtctatggt
    19801 ttttccgaca tccgcaccgt taaatattcc gaatgatgcg acatattcat aaagtgagcc
    19861 aaatctgact acaccgtcaa tatgggtcaa agaataaccg accatcacca gcggaccgac
    19921 aatacgataa tacgatgacg atccttccgt acctaaatcg cccagacggg tggaaatata
    19981 aggaaacgcg attaccacac caaccaggaa tacagccaga gaaatcaatg ctaacggtaa
    20041 ctttttctta atcgcctctt tattcagata ttgaaatgcc cactccagca aataaaacag
    20101 gataaaggtc ataacccctg aaaacgatcc ggataatatt atccctgcga gaatcatagc
    20161 atcggtttta ggcgttttga taccaaactg tttgatgctg agccaaattg agattaatgc
    20221 cagagcgaaa aatgccggtt cgaaataaag tgctgtcgtg cgcttgccgc cgaatttaat
    20281 gaaattcagt acatagctgt tgctgtaaat cagatatttc gaaatcgact ccataatact
    20341 gctgccgccg gtgaggataa tttgcgccat ctccaccgcc gccagcgcca ccaccaaccc
    20401 gaccaccaga taaaagaaac gtaatatctt gcgatggttg tgtggcgaaa ttgttttaaa
    20461 acgaatactc cacaccatgc caataatgat cacaatatag acaaacagca tagttgaagt
    20521 gacgtattta ctggcatcca gcgactgacc aaacagatag ttaaacgccg tgagtcccgc
    20581 accaatccct aaggcaatca tcaatttttt aacgctgatg cgctctaaaa acagcagtaa
    20641 caagacaggt aaaaaagtga cgatggtgat ggggaaactt tcgccaagct gggcgatttt
    20701 gacgttaacc agcagataaa tcagcggcaa caacaggtag ctacagattc tgatagaagt
    20761 tgacatactc ctccagcatc tgttgtccac tgtaggccgc gcggctgcgt tggctgaact
    20821 cagccagcgt ggtaccaaat atcgcctgcg cgatttccgg tttgcttaac tgcaccagtt
    20881 gcagcacgtc ttcttcgctg acggttttac cgccggattt ttgcagcact tcccgcgccg
    20941 cgtcgctatg ggtggcaatc accggcacgc caatcgatag cgcctcacac aaaatcagcg
    21001 ggtagttatc gacgcgagaa ctgaacacca gcgcatccat ctgattgagc gcgctcatca
    21061 acttgcgctt gtcagtttca aagccgtgat taaccacgtt gccagcggtg aacggcgaga
    21121 acttaccaaa ggtatgcagt tcgattttgt cgcccagcgc catcatctca cgcaccagtt
    21181 gctggttagt tttgccgtcg taacgcaggt catgcgccac caccgcgatt ttcggcttac
    21241 cctgggtttc gcgcaccgga ggcagatccg ccagaatcgc ttcggttgcc atatcaatac
    21301 cgttattgat aatccggcaa cgccctggac cgtacaggct attgaaagcg tcagccacat
    21361 gctggctggg ggaaataaac tgacagccca gcgccagcat ctcacggaat aactggcgtt
    21421 tgcccgccac cagctggtgc gcccgatcaa tcttcaccgg cggataatta tttaaggtcg
    21481 ggcatttctg gcagcccgtt ttccagcctt cacaaccgtc agtaaaggcg cagcgcccgg
    21541 taacgctcca gtggtcgtgc agcgtccaga ccagagtgac atccggtttg tggtttttca
    21601 ccttttcgca aaagcgcacc acgctcttaa gatttagcca gtagctgtgc agcacatgaa
    21661 aatgcaggac caccggaccc ggtgtgcaag taatggtgcg atataactca ttgaaattgc
    21721 caaacagatc gcgattaaac agacgaaaca acgcaatgtt cgccatcgcg gtcatccgcg
    21781 gcgtatgttt gacgacctgc ggatagtttt gatggctgac gctctctttg ccgcctttgc
    21841 cgtagccgta gacaaaatgt gacgccagcc cctgttgcag cgcacgctga tggagatcta
    21901 acgccacacc tgccgccccg ccttccgcca gtcgcacatt aaattgcaga atattcattt
    21961 aattaccttc actcgcgctt tttctcccac caccagcgcg ttgtccggga cagagtcgag
    22021 caccacgctg cccgcgccca cggtgacgtt gttaccaagc gtgatatcac caagaataat
    22081 gacgttggca ccgagttcga cgccgttgcc aatgtgtgga catgccatgt tatcggcacc
    22141 acgattgccg atagtgacgc cgtggcgaat ggtgaaatca tcccccgcta ccacgttttt
    22201 attgatcact acggcgtaac cgtgatggat agtaaagcgg cggccaatgg tcgcggcggc
    22261 ctggatttca taaccgaaaa agcattcggt gataatgcga tacagcacca gcagcggggc
    22321 cgcccacaga ttgttgagga cgttcttttt gcgccacacc gaacaaaaat gagcaacacg
    22381 ataggcaaga accatgcagc acgggcgtaa actccagctg ttggcacgca gatcttccag
    22441 catctttagc gcccccgaat accatcagcc agacgtttgc cgttacgcac cgacagcagc
    22501 gtcagcaaag tgcgccaggt catacgttta ttgcggatct ggtagagggt aaacagctga
    22561 tattttttgc ttgcacggtc aaatttatct ttgtgcttgc ggtaaaagtg gaagtacccg
    22621 gagaattttt tcggtgacga ggtgatctgc atttcaccgt gattgatatg caggatctgc
    22681 gtcgcctctt ccactttcca cggttcgccg tactccacca ccatccgcag gaagatgtcg
    22741 taatcctgtg ctgctttcag ttcggtatcg aacaaacact ctttgaaacg ccatgcccag
    22801 gtaaagacct gattgccaat gatattgcgt ttatagaaca ggcggcgtga gtacggcgat
    22861 ttgggataca gtggtaagct tgccggttgg gaataaactt ctccctggca tacgtagtcg
    22921 ttagcgtaca aaaaggcgtg tgtgaccagt tgctgtttat gggcgaggaa gacgctcaga
    22981 cggttgggtg tccattcgtc atcgtcatca atcccggtga tatattcccc ttgcgccagc
    23041 ataatcgcct ggttacgtac cgcgcacgcc ccgctgttaa tatcgttgtg aatgtaagtg
    23101 atacgcggat cgttgagggc ggtgacgtac tgttgcagtt gttcccagga ggtggagcaa
    23161 tcatccacga tgatcatctc ccagttgctg tagtcctggc gcaaaaccga ttttattgcc
    23221 cgaatcgcca gttgttggcg gttccaggtt ggcatataga ttgagatcag cggattgtct
    23281 ttcataggtt gttctcccgg cttatgagct ggtttgcttg ccggatgcgg cgtgagcgcc
    23341 ttatccggcc tacggggcgg tgcgaatgca ggcgtcgtta tttcgcatcc gacttatatt
    23401 cgtattcgta atagccataa tcctgatacg cgctggcgcg gcggaagatg gagttcagaa
    23461 tcaccccttt caccggaata ccattttgct caaagcggct cagactggtt tccacttctt
    23521 tcaatgtgtt gaccgcataa cgcgccacca ttaacgtggt tccaacatga cgaccaacaa
    23581 ttgccgcatc ggtcactgcc agaatcggcg gcgtatcaat caacaccagg tcatagtttt
    23641 tactcgccca gttcaccagt tcggcaaagc gttcgctcat caacagttca gaaggatttg
    23701 gcggcacctg accgcgcggg atcaggtcaa atttggcaat agaggtcggt ttagcagcgg
    23761 tagtaatatc gccctgacca atcagaattt ccgacaggcc attaacgtta ttagtgccca
    23821 acagctcgtg ggtgtagcct ttgcgcatat cgcagtcgat caacaacacg cgtttattgg
    23881 tctggctgat caccgccgcc aggttggcgc agacaaaggt tttaccgatt gacgggctaa
    23941 ccccggtcat catcaacaca ttgttctgcg cctgcatcat cgcgaagtgc aaactggtac
    24001 gaagactgcg gatggcttca atcgccagat cggttggatt ccccaccgcc agtagctggc
    24061 tctgtttata gcgtttaatc cctttgatgg ttttgacgct atcgcgcgct ttctgccatt
    24121 ccgacaacgg gatgctggca tagacgctga taccgtgttc ttccagcacc tgcgggcttt
    24181 cgatgccgcg attaaacaac gagcgcagca gcacacccac gatagagagc atcaggccaa
    24241 ggataatcgc gccgaggata atcagccctt tcttcggttt tagcacacca ggctgagtga
    24301 ttgccgggtc aacaatgcgc acatcgccga cggtgctggc ttcggtgatt ttcagctcct
    24361 gctctttatt cagcagttgc atatagacct gctgaccaga ttcgacatcg cgggtcagac
    24421 ggacaatttc ctgctgggtt ttcggcatcg ccgttacgcg accattaagt ttggcttttt
    24481 cgtcttccag cgcctgacgt ttctccagca gcgtgcggta cgcggggtga actttggtgt
    24541 acagcttgga gatttccgcc tctttaaagg tcagttcgtt caactgcgcg tcgatgttca
    24601 ccatcgaatc gagcaccgct ttcgcttcca gcggcagatc aacagaatct ttatcctgac
    24661 ggaaggcatt cagtttgttt tcggcaacat cgaggcggct acgtacttcc ggtaactgtt
    24721 gcgcgaggaa agcgaggctt ttcgacgctt ccgccgattt gcgctcaata ttttgttcct
    24781 gatagttacg ggcgatgctg ttaagaatgt cgcggatctg ttcacgatct tcaccggtat
    24841 aagtcaggct cagtacgcct gcgtctttgc cgttctccgt taccgtcagg ctgttttgca
    24901 gttgattgat catccccagc gtggagtatt tggtgacggt aaactcactg cccgggcggg
    24961 cgtgaatggc ttcaaccatc agcgtgacgc cttctttttt cagcatctgg cccgcttgcc
    25021 cacgggcgct aaagccgcca tcactgctca gggtgtagtt tttattgtcc agcacattaa
    25081 gcgtaaacac ctggtccgcc atctctttcg ggcggttaaa ggtggtcact ttcaccgtct
    25141 cgttctgacg tcccatcaaa cgatcccagc ccgcaccgaa aatcgggaac gtgtttttgc
    25201 tcaccgcaat atcgaggtct agatcgtcca ccgttttacc aagcaccaga cgcgaacgaa
    25261 tcaactggat ctcggcgtcc gatgcaggcg gtttgttggc taatgccgag ccaatgtcct
    25321 gcactaacga attgccgctg ttttgttcga tttgtaccag tgcgtcggca ctataaatcg
    25381 gcgtggcgaa gaaagtgtaa accacagcac agagggcaaa tacagcggtg atgccaatca
    25441 cccaccagcg cgcttcaatg acggtgccga ccaggcgacc aatatcgatt tcatcactgc
    25501 ccgttaccgg agcggcatgt tgttttactt tttctgtcat tcttatacct gctctgcgtt
    25561 caatgcctgc gcccactggc gggcagaccg ttcaagtaat gtgtacaccg ctgcaaacgt
    25621 ttcccggctt ttgcgatacg gatcggggat ttcacattcg ttatcccagt gaccaaacag
    25681 catcactttg ccgcgcatct caggtgccat ctcgcataag cgttcgatat ggcgcttttc
    25741 catggtcaaa atcaggtcat agttgcgaca caggcggcgg ctgatttgcc gtgcacagtg
    25801 accttccaga gacagttgat gttctgcggc gacgctgata gcggtaggat cagcgccctt
    25861 accgaccagc gcgccgagtc cagcggactc cactttcagc tccgggtgat aacgttgcag
    25921 taagcgttcc gccgtcgggg aacggcaaat attgccgaca cagacaacta agatgttgtt
    25981 aaacatgacg attaccagtt atgaatgtcg ctggctgtat ccgtcatgta acggacaccg
    26041 ctaatagttg gcagcaactg attgatcaga cggttccagc gggaaaccgg ggcggtggtg
    26101 acatacacca cgtcataagg ttgcaggcgg aattctgtcg ccatcaccag cgacgtcgca
    26161 tcggacatat ccagctggta gatattggca atcttgccgt tacgcccgcc ctcgcctttc
    26221 aacggacgaa tgacaaagat gccgctggcg ttggaggtgg tcatgtcgat gccttcggca
    26281 ttgcccagag cttcagtcag ggtcatgccg ctaaagtcca ttttcagggt gctctgtttc
    26341 ttcacttcac ccatcacaaa tactttcaga tcatcattac gcggcacgta gagaatatcg
    26401 ccgggataaa gcagtcggtt ctgattgagg tcgccgtttt gcatcagcgc ctgcaaagaa
    26461 atgcgttctt cacgaccatt gtgtgttagc accacgttgc gccagtcagc ggtgtcggtc
    26521 aggccacctg cggcgttgat ggcgtcgaga atggtcagtg gtacgttggt gatcgcctgt
    26581 tgaccggatt tattcacctg acctgagata taggcctttt gcgagcggaa ggcggcgata
    26641 ttaacgtcca cctgcgggtc agcgatgtac gtcgctaagc gcccggtaat atcactgcgg
    26701 atttcagcga gcgttttccc gactacgtga accttgccga tatacgggta aaacatagtg
    26761 ccgtcaggct gtacccagtt gccggtgtcg ctggagctgc ggtactgacc ggctggcgtg
    26821 gtgagttccg ggtgatccca gacggtgaca ttaagaacgt cccccggccc gacgcgatac
    26881 tgataattcg cgatctcgct ttccagcgtc atattggggc gcgctacatt cgggcgtggg
    26941 cgtaattggt caatcaggcg cggggtcagc ggataaacat tcaccatttt gtcgagatcg
    27001 aaatcagcgt cctgctgttt gatgacgtct ttgcccatcg tcgacatatt gctgcccgga
    27061 agtactgtgc aaccgcttat caaggttact gacaccaata atggcatcaa tttcattttg
    27121 gatttcatca ttgtttattt atcactttgg cagagtaatt atcctgtgca ctattaatag
    27181 caatgtcgcc atgcacattt accttgcagt taattgaata aaaatttaac tggcatcagt
    27241 cctaaaaaaa ttgatttcat ccgcaggcta ttgacagaat aattcagact ggtctttcag
    27301 gcatccagac acgctaccgc ccctggcttt ttagctacca atacactgat ttagcttaat
    27361 ttttcaaacc ctctctgcat acaatcgttg atgagaaagg gttattgcgg aaattaactt
    27421 ccgaatataa ggtgacatta tggtaattga atattggctc tccaataatg caggaggaag
    27481 tgttacagct aacggaatag caggcaaggt aacaattcgg taattggcta tttttaagaa
    27541 ttatattaag tgtcattcaa tatggttttt aggagtttct ttaggttgac aatatttaat
    27601 attgcgcctc cacatgcgat atttcttaaa taatgtttta ttattaccac ttttaattca
    27661 gggataatgt gaggttatta ccctcaaata atatgagata aatagtgctg caacattgca
    27721 tttttgcccc gatttatcca tgatcgaatt atgacatttg tcatacaacg aataggtttt
    27781 gtacttacta tggaatggat tgccgatccg tctatctggg ccgggttaat cacgctgatt
    27841 gtgatcgaac tggtcctcgg cattgataac ctggtcttta ttgccatcct cgccgaaaaa
    27901 ctaccgccga agcagcgcga ccgcgcacgg gttaccgggc tgctgctggc gatgttaatg
    27961 cgcctgttac tgctggcgtc aatctcctgg ctggtcaccc tgactcaacc gctgttcagc
    28021 ttctgctcgt ttacctttag cgcccgcgac ttaatcatgc tgtttggtgg tttcttcctg
    28081 ttgttcaaag ccacgatgga gctgaacgaa cggctggaag ggaaagacag caataatccc
    28141 acacaacgca aaggtgcgaa gttctggggc gtggtgacgc aaattgtggt actggacgcc
    28201 atcttctcac tcgactcagt gattaccgcc gtcgggatgg tcgaccattt actggtcatg
    28261 atggccgccg tggttatcgc tatcagcctg atgttgatgg ccagcaagcc gttaacgcaa
    28321 tttgttaaca gtcacccgac catcgttatt ctctgcttaa gcttcctgtt gatgattggc
    28381 tttagcctgg tggcagaagg tttcggcttc gtcattccga aaggctacct gtacgctgcc
    28441 attggtttct cggtgatgat cgaggcgctc aatcagttgg ctatctttaa ccggcgacgt
    28501 tttctttccg ctaaccagac gctgcgccag cggactaccg aagcggtaat gcgcctgctt
    28561 agcgggcaaa aagaagatgc ggaactggac gccgaaaccg cgtccatgct ggtggatcat
    28621 ggtaaccagc agatctttaa tccgcaggaa cggcggatga ttgagcgggt acttaatctt
    28681 aaccagcgta ctgtcagcag cattatgacg tcgcgccacg atattgagca tatcgatctc
    28741 aacgcgccgg aagaagaaat ccgccagttg ctggagcgaa atcagcatac gcggctggtg
    28801 gttaccgacg gtgatgacgc agaagatttg ctcggtgttg ttcacgttat cgacctgcta
    28861 caacagtcac tgcgcggcga accgctcaac ctgcgggtgt tgattcgcca gccgctggtg
    28921 ttcccggaaa ccttgccgtt gttacctgcc ctggagcagt tccgtaatgc ccgcacgcac
    28981 tttgcttttg tagtggatga gtttggctcg gtggaaggga ttgtgacatt aagtgacgtc
    29041 actgaaacca ttgccggtaa cttaccgaac gaagtggaag agatcgacgc ccgccatggt
    29101 attcagaaga atgcggacgg ttcctggacg gcgaatggtc atatgccgct ggaagatctg
    29161 gtgcaatatg tgccgctgcc gctggatgaa aaacgtgaat atcacaccat tgccgggctg
    29221 ttgatggaat atttacagcg tattccaaaa cctggcgaag aagttcaggt gggggattat
    29281 ttgcttaaaa cgttgcaggt ggaaagccat cgcgtgcaga aggtgcagat tattccgctg
    29341 cataaggatg gcgagttgga gtacgaggtg tgatggtatc ggtcatttta cccgatagcc
    29401 gaaaggaata agatgaggga accgcgccag cggcgattta ccgccgggag cccgggttgc
    29461 cagggtggtg gcgattgagc caccctggca cgttcaccgg cttgagcgtt ccatagtagt
    29521 gaggaacatc aagtgaacgg aacagccttc gatcttacat attcagaata taatggtgcc
    29581 gccgcgaacg cctgatgcga cgctttacgc gtcttatcag gcctacagga caggtcacct
    29641 ggtaacctac atcttctcca gcaacttctt cacatctttg ccattgcggg aatctttatt
    29701 ccgctcggcc cagtcattca ggcgacgttt cgcttcgtcc tgtagatgtt tgcgcagtaa
    29761 ctgatccact tgcagactgt aattgagttg ctgccagttg ccataaaccc gcagcggtac
    29821 tggcgtttct ttgaggaaat caatcagttt gctttccccg ttccagccac ccacgacccg
    29881 aatatcaaac tgggtgtcgc aggtttgatc tgccagattc aacatgcctt cccctgtcag
    29941 cgccagcaca ggcgattgac cttgcatgtc gtttaacgtc acgacgccat ccttcaacgt
    30001 caaatcggtg gtaaagcggt caagacgcgt tacgttatcg aagttttcag cggccttcac
    30061 atcaccacca ttacgttcta ccgcttgctg aatcatctgc tggaagttca tcccttccat
    30121 gcgcgtgtcg gtcatttcga catgtgcctg tccttgccag ttgtggcgga atgcgtcggc
    30181 atctatgtca gcaccggaga aatcaccagc cagtgacatt tttccggtca acgaaatcgg
    30241 atagttaaac gccttcagaa tggtaccaat ctcaacgttt tccagccgtg gctggaagtt
    30301 tatccgcgga tttattgatg tcgcgtccag cgtgcccggc agtgaaacct gtccaccgtt
    30361 aagcttgccc tgcagttgag taatttccag caaacccgac ttgttggtca tttgcgtggc
    30421 aacatctgta aaattcattc cgcgccagcg cacgttactg gcctgcaaca aaatatcagc
    30481 cgtaaagcct tgcagtccct gatatgccgg ttcatcaata cgcgaagaaa tgaccgggcg
    30541 cggcaatgtt gattggctct gcccctgctg cgcggcaccg ttttcaccat tcgctgtttc
    30601 attaagcggg atgaggttgt caagattcag ttgcgggaat tgcagtctca gctgccattc
    30661 aggtttctct gtcagcgtga cctgtgcttg cccgctcagc gtgctgtcat tggcggtcaa
    30721 gctgatttgg ttaaatgaaa ggcgtttatg tgactcctgc cactgggcct ggaaactccc
    30781 ctgcccctga ataccttgtt ttggtaaatc tgcaccctgc aactgccagt taatttgttc
    30841 aatagccgcc gttaaatcat gcggataatc agaagcatct accgtaccgt taagggatat
    30901 cgtgagatcg cgctgatcgc gattaacccg cccggagaac tcaaatgagc cacgatgttg
    30961 gggatcttgt tccatttgca ggcggatatt gcggattgtc acctgctcgt catcttcatg
    31021 ctggaacacc agcacgctgt ccgccacctt aagactggat atatcaaacg accatccgcg
    31081 atcgtctgac agatccggca aggtattgtc gcgcggtgca accggagcgt cttcactgcg
    31141 caccgcttcc gtctgcggcg tcagttggat cactgcccct tttagcatca cctgcttaac
    31201 gctcagttga tgactcagta gtggtaaaag cgccacgtcc agacgcatgt tgtcggcgcg
    31261 aaccagtggc tggcttgcgc cctgggcggt gagagacatt cgcccggaga ggatactaag
    31321 ctgcggccag acgtgccaac gcagtggccc gtcgagctgc aattgataac cgctacgtgc
    31381 agcaacttgc ttgaccatat agtcgcggaa atcattcgga ttcaccagca acactaacgc
    31441 agataacccg gccaccagca cgaccaggag tatcatcagc gtcgtcagaa atcgtctcat
    31501 ggtatcctca atgggcctga attagtcttt atcgattcgg ctggctaccg cgccctgctg
    31561 gttgcgatat ttcgcatctt cacggcggtt gtaaggtcgc gccgccgggc cggaaagcgg
    31621 ctcaaagctc agcgcaccaa ttaacatgcc cggacgcagc gccagcggca gcttaccgga
    31681 gttgtagaac tccagcacaa tgcaaccaga ccagcccgga tcgatacggt gcgcggtgac
    31741 gtgcaccatc agccccagac gcgccaaaga ggaacgcccg tccagccagc ccaccagatc
    31801 ggctggcagc gtcaccgact ccagcgtcac cgccagcgcc agctctcctg ggtgaagata
    31861 gaacgcctcg ccctcgtcga gaacgatctc atcgctcatc acgcggtcaa gcgcggcgct
    31921 cacttcatct ttgggaccgc tcagatcgat aaacgctgcc gtgtgaccac ggaaggtacg
    31981 aaatttattg cccaggcgta catccaccgt cgcgccgtta atacgctcca ctggcggacg
    32041 tgggttgatc gacaaacggc cttcatcaag ccaggcttca atatctcggt cacacagacg
    32101 catggcattt tctcctttcg cgcatcactc ccttaacgcc aattacgtca agggcatacc
    32161 aggttatcac tgaacggtac acaatttatc aagcttattc aaagaactga cttattttcg
    32221 ctttcaatat atcgatcgcg atgcggtttt tcccgccgcg cggcacgata atgtccgcat
    32281 attgtttaga aggctcaatg aattgcagga acatcgggcg cacggttttt tgatattgcg
    32341 ccatcacgga atccattgaa cgcccacgct cgttaacgtc acgcttgatg cggcgcatca
    32401 ggcagatatc cagcggggta tcaacgaaaa tggagaagtt aagttcgtca cgcaaacgcg
    32461 catccgtcag caacaaaatg ccttcgagaa tgatgacctt cttcggctca accgtcaccg
    32521 tttctttcat acgcgtatgt tcaacatagc tgtaaaccgg caggtcaatt gccgagccgc
    32581 gtttcaacgc ttgtaaatgc tcaagcaaca ggctgtgatc catcgcgctg ggatggtcgt
    32641 agttggtctt aacgcgttct tccatcgaca gatggctttg atctttgtaa tagcagtctt
    32701 cgggaattac gccgatgtgt tcatcaccga cttgctcacg taattcacga taaagggtac
    32761 tggcaataag actcttgccg gaagccgatg cgccagcgat accgataatg acgcactgat
    32821 gagactgatc agtcatatat ttagcgacct gattaacctg gatgttagga agggggcgac
    32881 gaagcgccaa acgcggcaat tatagggatt tcatccgcct gataccagtc gaatagcgct
    32941 gccgcgcgct cagagttaat tgttgacgaa gaattcccgg tggcaaatta cgttgatcag
    33001 ttttatacaa ggtaaaaaat gttatacgca gttgcgcaaa ttatccgcct ttacgtcact
    33061 ttatgagcaa ttcgcatata aaatgtaaaa cttttgtact agcataaaca cagaaacgaa
    33121 tactggcgac ctggtcttgc ggataaagcg gtaatgagca aacaatcaca gcatgtatta
    33181 attgccctgc cccacccgct gcttcacctg gtcagtttag gtttagtctc gtttatcttt
    33241 acccttttct cgcttgagct ttcgcagttt ggcacccaac tcgccccact gtggttcccg
    33301 acgtccatca tgatggtggc gttttatcgc catgccgggc gcatgtggcc gggaattgcg
    33361 ctgagctgct cgctgggaaa tatcgccgca tccatcctgc ttttttccac cagctcgctg
    33421 aacatgacct ggacgaccat caatattgtt gaagccgtgg tcggggcagt gctgctacgt
    33481 aaattgctgc cgtggtataa ccccttgcaa aatctggctg actggctgcg tctggcattc
    33541 ggcagcgcca ttgttccgcc tctgctaggg ggtgttctgg ttgtcctgct gacgcccgga
    33601 gacgatcctc tcagggcatt tttgatatgg gtactgtcag aatccatcgg cgctctggca
    33661 ctggtgccgc tgggattgtt atttaaacca cactatctgc tgcgccatcg caacccacgg
    33721 ttgctttttg agtcgctgct cacgttagcc atcacactga cgttaagctg gctttcgatg
    33781 ctgtatctgc cgtggccttt tactttcatt attgtgctgt tgatgtggag cgccgtgcgc
    33841 ctgccacgaa tggaagcctt tttgatcttc cttaccacgg tgatgatggt gtcgctgatg
    33901 atggccgcgg atccctccct gcttgctacg ccgcgtacat acctgatgag ccatatgccg
    33961 tggctaccgt ttttgctgat cctgctgccc gccaacatca tgacgatggt gatgtatgcc
    34021 tttcgtgcgg aacgcaaaca catttccgaa agcgaaaccc gttttcggaa cgccatggaa
    34081 tattccgcta tcggtatggc gttagtgggc accgagggac aatggctgca aaccaacaaa
    34141 gcgctctgcc agtttctcgg gtacagtcag gaagagctgc gcggactcac ctttcagcaa
    34201 ctgacctggc cggaggatct caataaagat ctccaacagg ttgaaaagct gataagcggt
    34261 gaaataaaca cctattcaat ggaaaaacgc tactacaacc gcaatggcga tgttgtctgg
    34321 gcgttgcttg ccgtctcact ggtgcgccac acggatggca cgccgctcta ttttatcgct
    34381 cagattgaag acattaacga gctaaaacgc accgaacagg tgaatcagca actgatggag
    34441 cgcatcacgc tggctaacga agcgggcggg attggcatct gggagtggga gttgaagccg
    34501 aatattttta gctgggataa gcggatgttc gagctgtatg aaattcctcc acatatcaaa
    34561 ccgaactggc aggtgtggta cgagtgcgtg ctgccggaag atcgccagca tgccgaaaaa
    34621 gtgattcgtt gcaatcacgc tcgcccttta aactggaatt tcgcattacc gtaaaagacg
    34681 gtattcgcca tatccgcgcc ctcgccaacc gggtactgaa taaagaaggc gaagtcgaac
    34741 gcctgctcgg cattaatatg gatatgactg aggttaaaca gcttaacgag gcattgtttc
    34801 aggaaaaaga gcgcctgcac ataacgctgg actccatcgg cgaagccgtg gtctgtattg
    34861 atatggcaat gaaaattacc tttatgaatc cagtggcgga gaagatgagc ggctggacgc
    34921 aggaagaagc attaggtgtt ccgctcctga cggtgttgca tattaccttt ggcgacaatg
    34981 gtcccctgat ggagaacatt tacagtgccg acacctcacg ttccgcgatt gaacaagatg
    35041 tggtgttgca ctgccggagc ggcggcagct acgacgtgca ttacagtatt acgccgttaa
    35101 gtactctgga cggcagcaat attggttcgg ttctggtgat tcaggacgtt accgaatcac
    35161 gcaaaatgct gcgccagctg agctacagcg cctcccatga tgcactgacg catctcgcca
    35221 atcgcgccag ttttgagaag caactgcgta tgctgctgca aacggtaaac agtacacatc
    35281 agcgacatgc cctggtgttt atcgatcttg atcgctttaa agcggtgaat gacagcgccg
    35341 ggcatgcggc gggcgacgct ttgctgcgcg aactggcgtc gttgatgctg agtatgctgc
    35401 gttccagcga cgtgctggcg cgactcggtg gggatgaatt tggtctgctg ctaccagact
    35461 gtaatgttga aagcgcgcgt tttatcgcta cacgcattat cagtgccgtg aatgactatc
    35521 actttatatg ggaaggccgt gtgcatcggg taggtgccag tgccgggatt accttgattg
    35581 atgacaacaa tcatcaggcg gcagaagtga tgtcgcaggc tgatatcgcc tgttatgcct
    35641 ccaaaaatgg tggacggggc cgggtgacgg tttacgaacc acagcaagct gccgcacata
    35701 gcgaacgggc ggcgatgtcg cttgatgaac agtggcggat gattaaagag aatcagttga
    35761 tgatgatggc ccacggtgtc gcttcgccgc ggatcccgga agcgcgtaat ttgtggctga
    35821 tttcgcttaa actctggagt tgcgaaggcg agattattga tgaacaaaaa tttcgtcgta
    35881 gcttcagcga tccggcactt agccatgctc ttgaccgacg ggtattccac gaatttttcc
    35941 agctggccgc aaaagcggtt gccagtaaag gcataagcat cgccctcccc ctttccgttg
    36001 ccggtttgag tagcgccacg ctggtgaatg atctgcttga gcagctggaa aatagccctc
    36061 taccaccacg gttattacat ctgattattc cggcagacgc gattttagat cacgcagaaa
    36121 gcgtgcaaaa actgcggctg gcgggatgtc ggatagtgct cagccaggtg ggccgcgatc
    36181 tgcaaatctt caactcgctg aaagcgaata tggcagatta cctgctactt gatggtgagt
    36241 tatgcgccaa cgtgcagggt aatttgatgg atgagatgct gattacgatt attcaggggc
    36301 acgctcagcg actcgggatg aaaaccatcg ccgggccagt cgttttaccc ttagtgatgg
    36361 atacgctttc tggcatcggc gtcgatctga tttatggtga tgtgattgcc gatgcccaac
    36421 cgctggattt gctggtgaat agcagttatt tcgcgattaa ctgagggagc gtttgcccat
    36481 tgcctgatgc gacgcatcag gcctacaatt cgtccgttcc gtctggttgc cagccttccg
    36541 tataccagat atgcaacagc gcataagaac gccagggctt ccagcgctcg gcataacggc
    36601 ggatttgtgc cggtgtcatc cccggaaatc gctgtttaat cagataatca tccggcagaa
    36661 aaacatcttt cgcctgccag ccacgcaaag cgaaataatt cgccgtccag cgcccgatac
    36721 ccggaaaagt ttgcagcgtt ttcatcgcct gctccacatc gcccggaatt gtcattggta
    36781 agctgccctc cagcgccgca tttgccagat gaatcagtgc ctctgcccgt ttcaacggca
    36841 tgcctaacgc ttttaatgcc tgcgggtcgg ctgctgccag ccgctgaggc gtggggaagc
    36901 agatatactc cggaaaatca tccagccgtt cgccataaag ctgtacaact ttggcagtca
    36961 attttgccgc catcgccacg ctcaccagtt ggcctaaaat cgcccgcaca ccctgctcaa
    37021 aagcatcaat acagccgggt aaacgcaatc cgggccgcgc cgcgcctaac ttgcccaacg
    37081 caccgttaac aatctgcggg ttacattgca gatcaaacag gcggctcatt ttcgccagac
    37141 actcagcggc aacgggttct aaacctgcac ttaaatttat gtgcagagta tggcgggcta
    37201 tatccggaat agcagtcacc acgccgcgat attcgcccac cgccagacta cgggcataat
    37261 aatcctcagc gaccgtttcc acaccactca ctgcacgggc ggcgagaaat cccaacatcc
    37321 acgaccagtc atacggcggc tgccagttca gggtatacat cgcatctcct tattcatccg
    37381 ctttcagcat aaacgttatt cagacgctac gctttgcttt catattccgg ttgtcgcgac
    37441 agcaacattt cgctaaagtc acgccccttc ttcaccggca tggggattaa ttcgtgttta
    37501 ttggttttga ttacggtaca gcaaactgtt cagtggcggt catgcgtgac ggtaaaccgc
    37561 aattactgaa aatggaaaac gacagcacgc tgctgccttc aatgctttgc gcgccaacgc
    37621 gtgaatcggt aagcgaatgg ctgtaccgcc atcatgatgt tccggcagac gacgatgaaa
    37681 cgcaggcgct gttacgtcgg gcgattcgtt ataaccgcga agaagatatc gatgttacgg
    37741 cgaaaagcgt gcagttcggt ctttcctcac tggcacagta cattgatgat ccagaagaag
    37801 tgtggtttgt gaaatcacca aaatcgttcc tcggtgccag cggcttaaaa ccgcagcagg
    37861 tagcgctgtt tgaggatctg gtctgcgcaa tgatgttgca cattcgccag caggcgcagc
    37921 tgccagaagc gattactcag gcggtgattg gtcgcccgat caacttccag gggctgggcg
    37981 gcgatgaagc aaacgcccag gcgcaaggga ttctggaacg cgcggcgaag cgtgccggat
    38041 tcagggacgt ggtattccag tacgagccgg tcgcggctgg gctggattac gaagccacct
    38101 tgcaggaaga aaaacgggtg ctggtggtgg atatcggcgg tggtacgact gactgttcat
    38161 tgctgctgat ggggccgcag tggcgttcgc gtctcgatcg tgaagccagc ctgctgggtc
    38221 acagtggttg ccgtattggc ggtaacgatc tggacatcgc actggcgttt aaaaacctga
    38281 tgccgttact gggcatgggt ggcgaaaccg aaaaaggcat cgccctgccg attctgccgt
    38341 ggtggaatgc ggttgccatc aacgacgtac ctgcgcagag tgatttctac agtagtgcca
    38401 acggtcgtct gcttaacgat ctggtacgcg atgcccgcga accggaaaaa gtggccctgc
    38461 tacagaaagt ctggcgtgag cgtttaagct atcgcctggt gcgtagcgca gaagagtgca
    38521 aaattgctct ttcaagcgta gcggaaaccc gcgcctcact gccgtttatc agcgatgaac
    38581 tggctacgct gattagccag caagggctgg aaagcgccct cagccagcca ctggcgcgga
    38641 ttctggaaca ggtgcaactg gccctggata acgcccagga aaaaccggac gttatctacc
    38701 tgaccggcgg tagcgcccgt tcgccgctga ttaaaaaagc gctggcagaa cagttgccgg
    38761 gaattccgat tgcaggcggc gatgactttg gctccgtcac cgccgggctg gcacgctggg
    38821 cggaagtggt gtttcgttaa aagaaaatgg ctcagcagcg gcttaaatct gcctacgcct
    38881 gagccagttt ttctgccgcc tgacgtaaag gttccatcaa cggcatggtc cgtgctggag
    38941 catgaaggcg gaatcgctgc aactctgtcg cgccgacagt caacgtcgat tccagccgat
    39001 gtcgtttggc ggtcattttc gccattaccg cttgtctgtc tgctggcgtt atcgcttcat
    39061 tcggcctgaa cacaaaacgg cgttcacagc tcgctttcca gtcgataacc gcctgggtca
    39121 gatggtcgcc aaaccctttc acttgcttaa cgccacgacg ggtaacatct gcggctgttt
    39181 caataccaaa agaccgtaac gccgctttac gcgcagggcc aacgccggga atagaggcaa
    39241 catcaataaa aaatccctcc agaaacttct gcttctgccg ttcccttgca gtgtcatgaa
    39301 gtgctgccag agcgcgtttc tcttcttccg gtaacccgag aatttcgtcc ttcatttttt
    39361 cgagcatcgc ccgtttggcg ataaaacctt ccagcccgcc caactgttgg atctggctga
    39421 ctaaatgctc atagtccatt ttcgcgcgat taaatgcctc acggcgttgc tggatttctg
    39481 ctttgtacgc tttgcttgtc agactgccaa taatccagat agccgacagc acaggaacca
    39541 aaataatata acgcggttct gcctgaaggc cgcaaagcaa cagcgataac gctgacagtg
    39601 cgatctcaat cagaatgatg tattcgcgcc gtaacaggcc taaaggaagc ggcctgccag
    39661 tcggttggaa atgatcgggt aatggcaatt gcaatgctgg cggtgctact gacgccatca
    39721 ccatcgccca gactttcgcc agcacaaaat caccgccggt ggtaatgacc tcttcgccga
    39781 gatcaataaa atagataacg ccttgattat ccagcgcaca ccacgggcag tcggtcaaat
    39841 gagcgggata aacatgcatt gccgaaacgg gacatttctt taactgttgg cgcagagaat
    39901 caagtgccgc tacccacgct ttagccgtcg gacgcccggt tgccacgcca ctttccgtga
    39961 atgcctgctg aaacatggct tcaacatcgc ccggtaacat cgatagcgga atcgatcgtg
    40021 gcggcggttt taaaccacgt cgctgattat ctgacgcgta ggcataacgg aaatgggtaa
    40081 tatccgtctc cagcgcatta cccgcatcag agataagcgg cacaccagaa taaggatgcc
    40141 ggccaccaaa caagacgtga aaaatcagca acgcaaggcc aaaattatcg tgattttctg
    40201 tgcgttcaaa gccgacaaat gacgacaagg tttgtagctc tggcggcgta aaatgcgaca
    40261 cgccgacttc gcataaatgc agtgtgccat tggcgttaat ctgaaaggag tcgctatcaa
    40321 tcagtaccac tttgctgtcg cgacctacca taaaactgtt ctggtttacg tcacccacga
    40381 cgtgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca acatagagta
    40441 gaaaatccca cgcacaatga gggtaacgtt gacgacgatg tgccgggcta tagatcatat
    40501 gaatcggttc tttaccagaa acttttggca tcataaaacc gataactttt ccgcctcgtc
    40561 caccgtgaag cgttgcctgc ggccaggcga cataattcaa caattgcgcg tcagctgtcg
    40621 cagccataaa ggcaagtttg tcctgtttta aggcgggtgg cggcgtgtga taaatcttgg
    40681 cgacgctatc gacaaactca ttgatatcat aaaccgcgcc ttcaccgcct ttgcccagtt
    40741 cacggcctgg cgtaacgcac tcaccagtag cagtatataa agtgggtttc atggtaacca
    40801 cagcgccagt gccaacgttt tatcatcatc cgtacgctcg tttaccgccg ggctggacaa
    40861 aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg acgccagtcc
    40921 attgaaaaac ggggtaaaga aaggtacatg aggagaatta tccagcatat ttaacgccag
    40981 tcgctggata ccatccgtaa atgctgcaac tttatgcaca cgctcagtgc tggtgaaagt
    41041 ttccagtctg gaaacggcat cttcatcggt aatgaagtgc gtcatattgg catattcacc
    41101 caccatcggc gtaagcggca attgcagacc gtgaccaaaa tccaccacca cgccgccatc
    41161 gccaatctgc ataatcaagg tgccattagc cgatgaaatc aatcccagaa acgtgcaggc
    41221 aaaatcgcga actgctaatt ctttggcttc cgcctcggca aaaagtcgct gacgaacggt
    41281 cagcacaata tccgtggcga ggatatcatt aagccccaat tccccaccct gcactttttg
    41341 cgacatataa gccatcgctt cattgacggc gagcgtcgcg ccttctcctc cctgcgaaac
    41401 gctacctgcg ccatcagcaa ggaacatcac cagtaatggc tgctggtcat ttagccaggc
    41461 aacctgcatc tggcaggcat cctggcaagg caagtccgcg ctgatgtgcg atgttccaac
    41521 agcggatgca taaaccagac gccagctcac actgacgtcc agccttttgg cgcttccagc
    41581 acaacttccg ttcccggcgt ggagcgggaa accgaacgca gcgaactgga taaccagcta
    41641 aacagttcac ggaattgtaa tccttgcaga ggcaaaggct gacgaacgct gatttgtgcc
    41701 agagtcttca tatccgcccc ctgaacaccg atggaaaaga aagcaaacct tttatcttct
    41761 tccccccgaa acactttgtt ggctgcagcc tgccactcat cggttggtgc accatcggta
    41821 atcaggaaaa tccacgggcg ataatacgaa ataccatttg cacgatactc acgtttccgc
    41881 tcctctacca tatccagggc tttggtaatg gccgcaccca ttggtgtatc gccctgggca
    41941 aacaggatgg gcggaaaaaa attagcggcg ctggtaaagg gttgttccac atgcaccggg
    42001 ccgaacgtca caatgccgag ttccactctt tttaaagcca gcggatcggc aagcagttca
    42061 tcacgaaagg tcaccaatcc ggcgttaagt tcattaatag gtctgccgct catcgagcca
    42121 gaaacatcca gcagcaaaat gcaggggcaa cgtggttccg ggttactggc aaaatcgctg
    42181 gtggcaaacg tgatttgttc gctcataaga aatcctttct tagagaatta tcgaggtccg
    42241 gtatcatacc gactgcaata atgaaataag agcaaacgaa gaaactttca gaattaggcc
    42301 cggcggcgca gaaaattttg ctattcgcct cgcaagaacg gagagccgtc gattgacact
    42361 aagggcggag tgacataatt tcaggagtga gggttaggga gaggtttccc cctccccctg
    42421 gtgttcttag taagcctgga agctaatcac taagagtatc accagtatga tgacgtgctt
    42481 catcataacc ctttccttat taaaagccct cttctccggg agaggctttc ccgtttcagc
    42541 gtcccgctga aatcatcggc ttacctcctt tcgccctgaa tgcagtctat cgctaacgcg
    42601 tgaataaaat agtttcctct gtgattattg cgtaacgact cgcaaagaat atttgctggc
    42661 aggatcgcag actacaaagc ctgcggattg acaatcttat cgtgaaggca tactttcagg
    42721 agtgagggta gagcggggtt tcccccgccc tggtagtctt agtaagcggg gaagcttatg
    42781 actaagagca ccacgatgat gagtagcttc atcatgaccc tttccttatt taaggcccct
    42841 tcctcgggag gggctttccc gtttcagcgt cccgctgaaa tcgtcggctt acctcctttc
    42901 gccatgcact tagtctatcg ctgacttgca gataaaatag tttccagtgt gattattgcg
    42961 taacgtctcg caaagaattt ttgctggcag gatcgcagac ggcaaagcct gcggattgac
    43021 aatcttatcg tgaaggcata ctttcaggag tgagggtaga gcggggtttc ccccgccctg
    43081 gtagtcttag tatgctgggt agctaatcac taagagcacc acgatgatga gtagcttcat
    43141 catgaccctt tccttattta aggccccttc ctcgggaggg gctttcccgt ttcagcatcc
    43201 ggctgaaatc tgcggcttac ctcctttcgc cctgtatgca gtctatcgct aacgcgtaga
    43261 taaaatagtt tcctgtgtta ttactggatg cgtgctcgca aatgtgcccg ccattcagac
    43321 gattcccgat agtgtttcat aattcctcca tttttctccc ttattggctg gctacactag
    43381 tatcattccg cgaaacgttt caggaagaga aactcttaac gatgaaaggc agttataaat
    43441 cccgttgggt aatcgtaatc gtggtggtta tcgccgccat cgccgcattc tggttctggc
    43501 aaggccgcaa tgactcccgg agtgcagccc caggggcgac gaaacaagcg cagcaatcgc
    43561 cagcgggtgg tcgccgtggt atgcgttccg gcccattagc cccggttcag gcggcgaccg
    43621 ccgtagaaca ggcagttccg cgttacctca ccgggcttgg caccattatc gccgctaata
    43681 ctgttacggt gcgcagccgc gtggacggtc aactgatggc gttacatttc caggaaggcc
    43741 agcaggtcaa agcaggcgat ttactggcag aaattgaccc cagccagttc aaagttgcat
    43801 tagcacaaac ccagggccaa ctggcaaaag ataaagccac gcttgctaac gcccgccgtg
    43861 atctggcgcg ttatcagcaa ctggcaaaaa ccaatctcgt ctcccgtcag gagctggatg
    43921 cccaacaggc gctggtcagt gaaaccgaag gcaccattaa ggctgatgaa gcaagcgtcg
    43981 ccagcgcgca gttgcaactc gactggagcc gcatcaccgc accagtcgat ggtcgcgttg
    44041 gtctcaagca ggttgatgtt ggtaaccaaa tctccagtgg tgataccacc ggaattgtgg
    44101 tgatcaccca gacgcatcct atcgatttag tctttaccct gccggaaagc gatatcgcta
    44161 ctgtagtgca ggcgcaaaaa gccggaaaac cgctggtggt agaagcctgg gatcgcacca
    44221 attcgaagaa attaagtgaa ggcacgctgt taagcctcga taaccaaatc gatgccacaa
    44281 ccggtacgat taaagtgaaa gcacgcttta ataatcagga tgatgcgctg tttcccaatc
    44341 agtttgttaa cgcgcgcatg ttagtcgaca ccgaacaaaa cgccgtagtg atcccaacag
    44401 ccgccctgca aatgggcaat gaaggccatt ttgtctgggt gctgaatagc gaaaacaagg
    44461 tcagcaaaca tctggtgacg ccgggcattc aggacagtca gaaagtggtg atccgtgcag
    44521 gtatttctgc gggcgatcgc gtggtgacag acggcattga tcgcctgacc gaaggggcga
    44581 aagtggaagt ggtggaagcc cagagcgcca ccactccgga agagaaagcc accagccgcg
    44641 aatacgcgaa aaaaggagca cgctcctgat gcaggtgtta cccccgagca gcacaggcgg
    44701 cccgtcgcgc ctgtttatta tgcgtcctgt ctccaccacg ctgctgatgg tggcgatctt
    44761 actcgccggg attatcggtt atcgcgccct gcccgtttcg gcgctgccgg aagtggacta
    44821 tccgaccatt caggtggtca cgctctaccc aggtgccagc ccggatgtca tgacctctgc
    44881 cgttaccgcg ccgctcgaac gccagttcgg gcagatgtct ggcctgaaac agatgtcgtc
    44941 gcaaagttcc ggcggtgcgt cagttatcac tttgcagttc cagctaacat taccgctcga
    45001 tgtcgccgag caggaagtgc aggccgcgat taacgctgcg accaacttgt tgccgagcga
    45061 tctgcctaac ccgccggttt acagcaaagt gaacccggca gatccgccga tcatgacgct
    45121 cgccgtcacc tcaaccgcca tgccgatgac gcaagtggaa gatatggtgg aaacccgcgt
    45181 cgcgcagaaa atctcgcaga tttccggcgt cggcctggtg acgctttccg gcggtcagcg
    45241 tccggctgtt cgcgtcaaac ttaacgctca ggcgattgcc gccctcggcc tgaccagcga
    45301 aaccgtgcgc accgccatta ccggcgctaa cgttaactcg gcaaaaggta gcctcgacgg
    45361 cccttcccgt gcggtcacgc tttccgcgaa cgaccagatg caatccgccg aagagtatcg
    45421 ccagctaatc atcgcctacc agaacggcgc gccaattcgt ctgggcgatg tcgcaactgt
    45481 agagcaaggt gcagaaaaca gctggctcgg cgcgtgggcg aacaaagaac aggccattgt
    45541 gatgaatgtt cagcgccagc ccggtgctaa cattatctcc accgccgaca gcattcggca
    45601 gatgctgcca cagctcactg agagtctgcc gaaatcggtg aaggtgacag tgctttccga
    45661 tcgcaccacc aatatccgcg catccgtcga tgatactcag tttgaattga tgatggctat
    45721 cgcgctggta gtcatgatta tctacctgtt tttgcgcaat attccggcga ccatcattcc
    45781 cggtgttgct gtaccgctgt cgttaatcgg cactttcgcg gttatggtgt ttctcgattt
    45841 ttcaatcaat aacctgacac tgatggcgtt aactatcgcc accggattcg tggtcgatga
    45901 cgccatcgtg gtgatcgaaa acatttcccg ctatatcgaa aaaggcgaaa aaccgttggc
    45961 ggcggcgctc aagggcgcag gtgaaatcgg ctttaccatt atctcgctga ccttctcact
    46021 gattgcggtg ttgatcccac tgctgtttat gggcgatatc gtcgggcgac tgttccgcga
    46081 atttgctatt acgctggcgg tagcgatttt gatctcagcg gtggtgtcgc tgaccctgac
    46141 accgatgatg tgcgcgcgga tgctcagcca ggagtcgttg cgtaaacaga accgcttctc
    46201 ccgtgcctcg gaaaaaatgt tcgacaggat aatcgcctcc tatggtcgtg gactggcgaa
    46261 ggtgctgaat catccgtggc tgaccttaag cgtggcgctc agcacgctgc tgcttagcgt
    46321 tctcttatgg gtgttcattc cgaaaggttt cttcccggta caggacaacg gcattattca
    46381 gggcactttg caggcaccgc aatccagctc ctttgccaat atggcccagc gacaacgcca
    46441 ggtcgcggac gtgattttgc aggatccggc agtgcaaagc ctgacctcat ttgttggcgt
    46501 tgatggcact aacccgtcgc tgaatagtgc acgtttacaa atcaacctca aaccgctgga
    46561 tgaacgtgat gaccgagtgc aaaaagtcat tgcccgtctg caaacggcgg tggataaagt
    46621 gccgggcgtc gatctcttcc tgcaaccaac gcaggatctg actattgata ctcaggtcag
    46681 ccgcacccag taccagttta ccttgcaggc cacctcactg gatgcgctca gtacctgggt
    46741 gccacagttg atggaaaaac tccagcaact gccacagctt tctgatgtct ccagcgactg
    46801 gcaggacaaa gggctggtgg cgtatgtcaa tgttgatcgc gacagcgcca gccgtctggg
    46861 gatcagcatg gcggatgtcg ataacgccct gtacaacgcg tttggtcagc gactgatttc
    46921 cactatttat actcaggcta accagtatcg cgtagtgctg gagcacaaca ccgaaattac
    46981 cccgggcctc gcggcgctgg ataccattcg cctgaccagt agcgacggcg gcgtggtgcc
    47041 gttaagctca attgccaaag ttgaacagcg ttttgcgccg ctttccatca accatctgga
    47101 tcagttcccg gtaacgacca tctcctttaa cgtgccggat aactattcgc tgggcgatgc
    47161 ggtgcaggcg attatggaca ccgaaaagac gctgaatctg ccggtggata tcaccacgca
    47221 atttcagggc agcaccctcg ccttccagtc ggcgctgggc agcactgtct ggctgattgt
    47281 cgcggcggtg gtggcgatgt atatcgtgct cggcattctg tacgagagct ttattcaccc
    47341 gatcaccatt ctctcgacgc tacccaccgc aggggtcggc gcgctgctgg cattaatgat
    47401 tgccggtagc gaactggatg tgattgcgat tatcggcatt attttgctga tcggtattgt
    47461 gaagaagaac gccatcatga tgatcgactt cgcgctggct gctgagcgcg agcaaggcat
    47521 gtcgccgcgc gatgcaatct accaggcttg tctgttgcgt tttcgtccga tcctgatgac
    47581 caccctggcg gctctgcttg gcgcgctgcc gctgatgttg agtaccgggg tcggcgcgga
    47641 actgcgtcgt ccgttaggta tcggcatggt cggcggtctg attgtcagcc aggtgctgac
    47701 gctgttcacc acgccggtga tttatctgct gttcgaccgc ctggcattgt ggaccaaaag
    47761 tcgctttgct cgccatgaag aggaggcgta agtgaagttt tttgccctct tcatttaccg
    47821 cccggtggcg acgattttac tgtcggtcgc cattaccctg tgcggcatac tgggcttccg
    47881 tatgctgccg gtcgccccgc tgccgcaggt cgattttccg gtgattatgg tcagcgcctc
    47941 gctgcccggt gcgtcaccag aaacaatggc gtcttccgtt gccacgccgc tggagcgctc
    48001 acttgggcgc attgccggag tcagtgaaat gacctccagc agttcgctcg gcagcacgcg
    48061 tattattttg cagtttgatt ttgaccggga tatcaacggc gcggcgcgcg atgtgcaggc
    48121 ggcgatcaac gctgcacaaa gtttgctgcc cagcgggatg cccagccgtc cgacctatcg
    48181 caaagctaac ccgtcggatg cgccaattat gatcctcacg ctgacatccg atacttattc
    48241 gcagggtgaa ctgtacgatt ttgcctcgac gcagctggct ccgacgattt cgcaaatcga
    48301 cggtgtcggt gatgtcgatg ttggcggcag ctcgctgccc gccgtacgcg tcgggctgaa
    48361 tccgcaggcg ctgtttaatc agggcgtctc gctggacgac gtacgcaccg ccatcagcaa
    48421 tgccaacgtg cgtaaaccgc agggggcgct ggaagatggc actcaccgct ggcagatcca
    48481 gaccaatgat gagctaaaaa ccgccgccga atatcagccg ttgattattc attacaacaa
    48541 cggcggcgcg gttcgtctgg gcgatgtggc gacggtgacc gactcggtgc aggatgtgcg
    48601 caacgccggg atgaccaacg ccaaaccggc tattttgctg atgatccgca agctgccgga
    48661 agccaatatt atccagacgg tagacagcat ccgggcaaaa ttaccggagt tgcaggaaac
    48721 cattccggcg gcgattgatc tgcaaattgc tcaggatcgc tcccccacca ttcgcgcctc
    48781 gctggaagaa gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc tggtggtgtt
    48841 tttattcctg cgctcgggtc gcgccactat tattcccgcc gttgcggtgc cggtttcgct
    48901 gattggtacg tttgcggcga tgtacctgtg cggattcagt ctcaataacc tttcgttaat
    48961 ggcgctcacc atcgctactg gtttcgtggt ggatgacgcc atcgtggtgc tggaaaacat
    49021 tgcccgtcat ctggaagcgg ggatgaaacc gctacaggcc gcgctacaag gtactcgcga
    49081 agtcggcttt acggtgctat cgatgagtct gtcactggtg gcggtgttcc tgccgctgct
    49141 gttgatgggc ggattgccgg gccgactgtt acgcgaattt gccgtgacgc tttctgtcgc
    49201 cattgttata tcgttgctgg tttctctgac attaacgcca atgatgtgtg gctggatgct
    49261 gaaagccagc aagccgcgtg agcaaaagcg actgcgtggt tttggccgca tgttggtagc
    49321 cctgcaacaa ggctacggca agtcgctgaa atgggtgctc aatcataccc gtctggtggg
    49381 tgtggtgctg cttggcacca ttgcgctgaa tatctggctg tatatctcga tcccgaaaac
    49441 cttcttcccg gagcaggaca ccggcgtgct gatgggcggg atacaggcgg atcagagcat
    49501 ttcgtttcag gcgatgcgcg gcaaattgca ggatttcatg aaaattatcc gtgacgatcc
    49561 ggcagtggat aatgtcaccg gctttaccgg cggttcacga gttaacagtg ggatgatgtt
    49621 tatcaccctc aagccacgcg acgaacgcag cgaaacggcg cagcaaatta tcgaccgtct
    49681 gcgcgtaaaa ctggcgaaag aaccgggggc gaatctgttc ctgatggcgg tacaggatat
    49741 tcgcgttggt gggcgtcagt cgaacgccag ctaccagtac acgttgttat ccgacgacct
    49801 ggcggcactg cgcgaatggg agccgaaaat ccgcaaaaaa ctggcgacgt tgccggaact
    49861 ggcggacgtg aactccgatc agcaggataa cggcgcggag atgaatctgg tttacgaccg
    49921 tgacaccatg gcacggctgg gaatcgacgt acaagccgcc aacagtctgt taaataacgc
    49981 cttcggtcag cggcaaatct cgaccattta ccagccgatg aaccagtata aagtggtgat
    50041 ggaagtggat ccgcgctata cccaggacat cagtgcgctg gaaaaaatgt tcgttatcaa
    50101 taacgaaggc aaagcgatcc cgctgtcgta tttcgctaaa tggcaaccgg cgaatgcccc
    50161 actatcggtg aatcatcagg gattatcggc ggcctcgacc atttcgttca acctgccgac
    50221 cggaaaatcg ctctcggacg ccagtgcggc gatcgatcgc gcaatgaccc agcttggtgt
    50281 gccttcgacg gtgcgcggca gttttgccgg aacagcgcag gtgttccagg agacgatgaa
    50341 ctcacaggtg atcctgatta tcgccgccat cgccacggtg tatatcgtgc tgggtatcct
    50401 ttacgagagt tatgtgcacc cgctgacgat tctctccacc ctgccctcgg cgggtgttgg
    50461 agcgctgctg gcgctggagc tgttcaatgc cccgttcagc ctaatcgccc tgatagggat
    50521 catgctatta atcggcatcg tgaagaaaaa cgccattatg atggtcgatt ttgcgcttga
    50581 agcccaacgg cacggtaacc tgacgccgca ggaagctatt ttccaggcct gtctgctgcg
    50641 ttttcgcccg attatgatga ctaccctggc ggcgctgttt ggcgcgctgc cgctggtgtt
    50701 gtcgggcggc gacggctcgg agctgcggca acccctgggg atcaccattg tcggcggact
    50761 ggtaatgagc cagctcctta cgctgtatac cacgccggtg gtgtatctct ttttcgaccg
    50821 tctgcggctg cgtttttcgc gtaaacctaa acaaacggta accgagtaaa tgacagatct
    50881 tcccgacagc acccgttggc aattgtggat tgtggctttc ggcttcttta tgcagtcgct
    50941 ggacaccacc atcgtaaaca ccgcccttcc ctcaatggcg caaagcctcg gggaaagtcc
    51001 gttgcatatg cacatggtca ttgtctctta tgtgctgacc gtggcggtga tgctgcccgc
    51061 cagcggctgg ctggcggaca aagtcggcgt gcgcaatatt ttctttaccg ccatcgtgct
    51121 gtttactctc ggttcactgt tttgcgcgct ttccggcacg ctgaacgaac tgttgctggc
    51181 acgcgcgtta cagggcgttg gcggcgcgat gatggtgccg gtcggcagat tgacggtgat
    51241 gaaaatcgta ccgcgcgagc aatatatggc ggcgatgacc tttgtcacgt tacccggtca
    51301 ggtcggtccg ctgctcggtc cggcgctcgg cggtctgctg gtggagtacg cgtcgtggca
    51361 ctggatcttt ttgatcaaca ttccggtggg gattatcggt gcgatcgcca cattgatgtt
    51421 aatgccgaac tacaccatgc agacgcggcg ctttgatctt tccggctttt tattgctggc
    51481 ggttggcatg gcggtattaa ccctggcgct ggacggcagt aaaggtacag gtttatcgcc
    51541 gctggcgatt gctggcctgg tcgcagttgg cgtggtggca ctggtgcttt atctgctgca
    51601 cgccagaaat aacaaccgcg ccctgttcag tctgaaactg ttccgcactc gtaccttttc
    51661 gctgggcctg gcggggagct ttgccggacg tattggcagt ggcatgttgc cctttatgac
    51721 accggttttc ctgcaaattg gcctcggttt ctcgccgttt catgccggac tgatgatgat
    51781 cccgatggtg cttggcagca tgggaatgaa gcgaattgtg gttcaggtgg tgaatcgctt
    51841 tggttatcgt cgggtactgg tagcgaccac gctgggtctg tcgctggtca ccctgttgtt
    51901 tatgactacc gctctgttgg gctggtacta cgttttgccg ttcgtcctgt ttttacaagg
    51961 aatggtcaac tcaacgcgtt tctcctccat gaacaccctg acgctgaaag atctcccgga
    52021 caatctggcg agcagcggaa acagcctgct gtcgatgatt atgcaattgt cgatgagtat
    52081 cggcgtcacc atcgccgggc tgttgctggg actttttggt tcgcagcatg tcagcgtcga
    52141 cagcggcacc acacaaaccg tctttatgta cacctggctt agcatggcgt tgatcatcgc
    52201 ccttccggcg ttcatctttg ccagagtgcc gaacgatacg catcaaaatg tagctatttc
    52261 gcggcgaaaa aggagcgcgc aatgaagttc tggcgacccg gtattaccgg caaactgttt
    52321 ctggcgattt tcgccacctg cattgtcttg ctgatcagta tgcactgggc ggtgcgtatc
    52381 agttttgagc gtggctttat tgattacatc aagcatggta atgaacaacg attacaactg
    52441 ttaagtgatg cgcttggcga gcagtatgcg cagcatggca actggcgctt cctgcgcaac
    52501 aatgatcgct ttgtctttca gatcctgcgt tcatttgaac acgataattc ggaagataaa
    52561 cccggcccgg gtatgccacc gcacggctgg cgtacccagt tctgggtggt tgatcaaaac
    52621 aacaaagtgc tggttggtcc gcgagcgccg attccacctg acggtacacg gcgacccatt
    52681 ctggtcaacg gtgcggaagt tggcgcggtg atcgcctccc ccgttgagcg gttaacgcgc
    52741 aatactgata tcaatttcga taaacaacag cggcaaacca gctggctgat cgtcgccctg
    52801 gcaacgttac tcgcggcgct cgccaccttt ctgctggcgc gcggtttgct ggcaccggta
    52861 aaacgacttg tcgatggcac gcacaaactg gcggcgggcg atttcactac ccgcgtaacg
    52921 cccaccagtg aagatgaact gggcaaactg gcgcaagact tcaaccagct tgccagcacg
    52981 ctggagaaaa accagcaaat gcggcgcgat tttatggccg atatttcaca cgaactgcgt
    53041 acgccattag cggtgctgcg cggtgaactg gaagccattc aagatggcgt gcgcaaattc
    53101 acgccggaga cggtggcgtc tttacaggcg gaagtcggta cactgactaa actggttgac
    53161 gatcttcatc agttgtcgat gtctgatgaa ggcgctctcg cctatcaaaa agcaccggta
    53221 gatttgatcc cactgctgga agtggcgggc ggcgcatttc gcgaacgatt tgccagccgt
    53281 ggcctgaaac tgcagttttc cctgccagac agtattaccg tatttggcga tcgcgaccgt
    53341 ttaatgcagt tattcaataa cttactcgaa aacagcctgc gctacactga tagcggcggc
    53401 agcctgaaaa tctctgccga gcagcacgac aaaacggtgc gcctgacctt tgccgacagt
    53461 gcgccaggtg tcagtgacga gcagctacaa aaattatttg aacgttttta tcgcaccgaa
    53521 ggttcccgca accgcgccag cggtggttcc gggctggggc tggcgatttg cctgaacatt
    53581 gttgaagcac ataatggccg cattattgct gcccattcgc cttttggcgg ggtaagcatt
    53641 acagtagagt taccgctgga acgggattta cagagagaag tatgaccgag ttaccaatcg
    53701 acgaaaacac accgcgtatt ttgatcgtgg aagatgaacc gaagctgggg cagttgctca
    53761 ttgattatct gcgtgctgcg agctatgcgc cgacgcttat cagccacggc gatcaggtac
    53821 tgccgtatgt gcgccagaca ccaccggatc tgatcctgtt agatctgatg ctccctggca
    53881 ccgatggcct gacgctgtgc cgggaaattc gtcgtttttc tgacattccg atcgtgatgg
    53941 tgacggcaaa aatcgaagag atcgatcgcc tgctggggct ggagattggc gcagatgatt
    54001 atatctgtaa gccgtacagc ccacgggaag tggtagcgcg cgtcaaaacc attttgcgcc
    54061 gttgcaaacc gcagcgcgag ttgcagcaac aggatgctga aagcccgttg attatcgacg
    54121 aaggtcgttt tcaggcttca tggcgcggta aaatgcttga cctgacgcct gcggaatttc
    54181 gtctgctgaa aacgctctct catgaaccag gaaaagtgtt ctcccgcgag catttgctca
    54241 atcatcttta tgacgactac cgcgtagtaa ccgaccgcac catcgacagc cacattaaaa
    54301 acctgcgccg caagctggaa tctctcgacg ccgaacagtc atttatccgc gccgtttacg
    54361 gcgttggtta ccgctgggaa gccgacgcct gccgcatcgt ttagttttag cgacatgact
    54421 ttgttagccg gagatggcgt tcagaccaga tccggcaaca ttatcccacg catggtcagc
    54481 aaactgacat tatctctccg ccctggcagg catcagaaaa tcgctcatac tttaatcggt
    54541 aaacagcacc tttagatgct gttttcgata cgcaatttca atcaaggagt cattatggct
    54601 ggttggtttg aactcagtaa gagcagtgat aatcagttcc ggtttgtgct aaaagcgggc
    54661 aatggtgaga ctatcctcac cagcgagctg tatacctcaa aagcctctgc ggaaaagggc
    54721 atcgcgtcgg tgcgtagcaa cagcccgcaa gaagaacgct atgagaaaaa aacggcaagt
    54781 aacggcaaat tctatttcaa tctgaaagcc gctaatcatc aaattatcgg ctccagccag
    54841 atgtacgcca ccgcgcaatc tcgtgaaacc ggaattgcct ccgttaaagc caatggcaca
    54901 agccagacgg tgaaagacaa tacgtaatta cgatgccggg cgtgggttgt tggcgtccgg
    54961 ctttatatcc aaataacgct tagttattaa taattaagag taaccgccgc cattaaaata
    55021 gtgggcactg acgagcactc gttgtgcttt ctttgtttac catcccatac ttatcctcct
    55081 gaacatgttc atgtgccaaa atatcttcta atactttcgt gacgctatcc ggtttatgac
    55141 atacactgat gtaagatttt tacttacagg cgggcgtaaa atggccagaa cgatgacggt
    55201 tgatctcggc gatgagttgc gtgaatttat cgagtcctta attgaatccg gcgactatcg
    55261 cacccaaagc gaagtgatcc gtgagtcact gcgattactt cgagaaaagc aagcagagtc
    55321 tcgcctgcaa gcgttacgtg acatgctcgc agaagggttg agtagtggtg aagctcagcc
    55381 gtgggaaaag gacgcgttct tacggaaggt gaaagcaggg ataaggaaat aatgtgcgta
    55441 ttattaaact catgccaaaa gccaatgagg atttagaagg catctggtat tacagctatc
    55501 accatttcgg cgaaccacaa gccgacagat atgtggaaca tctttcagac gttctccaga
    55561 ttctgagtaa caataatata ggtacgccca gacctgaatt aggcgaaggt atttttgttc
    55621 ttccttttga acgccacgtc atttatttcc tgcaatcacc aggggaaatt atcgtcattc
    55681 gtatccttaa ccaaaaccag gacgccacca gacatttaca ctggagctaa acccgccgat
    55741 tgcgctacaa tgcccgccct taaagtgggg gcactcccct aaccgcttca tcaggtgaag
    55801 cggatctgac ctgtcatcag aacgagagaa ttatgtttaa accggaactc ctttccccgg
    55861 cgggaacgct gaaaaatatg cgttacgctt tcgcttatgg cgcagatgct gtttatgcgg
    55921 gccagccgcg ttattccctg cgtgtgcgca acaacgaatt caaccacgaa aatcttcagc
    55981 tcggcatcaa tgaagcccac gcgctgggga aaaagtttta tgtcgtggtc aacattgcac
    56041 cgcacaacgc caagctgaaa acctttatcc gtgacctgaa accggtggtg gaaatggggc
    56101 cggatgcgct gattatgtcc gatccagggc tgattatgct ggtgcgtgag cacttccctg
    56161 aaatgccgat ccacctttcg gtgcaggcta acgccgtgaa ctgggcgacg gtgaaattct
    56221 ggcagcaaat gggcctgacc cgcgtgatcc tctctcgcga gctgtcgctg gaagagattg
    56281 aagagatccg caatcaggtg ccggatatgg agatcgagat cttcgttcac ggcgcgctgt
    56341 gcatggccta ctccggtcgc tgcctgctct ctggctatat caacaagcgc gacccgaacc
    56401 agggcacctg caccaacgcc tgccgctggg agtacaacgt ccaggaaggg aaagaagatg
    56461 atgttggcaa catcgtacac aagtacgagc cgattccggt gcaaaatgtt gagccgacgc
    56521 tgggtatcgg cgcaccaacc gacaaagtgt ttatgatcga agaggcccag cgtccgggcg
    56581 agtatatgac cgcgtttgaa gatgagcacg gcacttacat catgaactcg aaagatctgc
    56641 gcgccatcgc ccatgtagaa cgcctgacca aaatgggcgt gcattcgctg aaaatcgaag
    56701 gtcgtaccaa atctttctac tattgtgcac gcaccgcaca ggtttaccgc aaagctatcg
    56761 atgacgccgc tgcgggaaaa ccgttcgata ccagcctgct ggaaactctg gaaggtctgg
    56821 cgcatcgtgg ctataccgaa ggtttcctgc gtcgtcatac tcacgacgat tatcagaact
    56881 acgaatacgg ttattcagtt tctgaccgcc agcagtttgt tggtgagttt accggtgagc
    56941 gcaaggggga cctcgcggcg gtagcggtga aaaataaatt ctccgttggc gacagccttg
    57001 agctgatgac gccgcaaggc aacattaatt ttacccttga gcacatggaa aacgccaaag
    57061 gcgaagctat gccgatagca ccaggcgatg gttatactgt gtggctcccg gtcccgcagg
    57121 atcttgagct caattacgcg ctgctgatgc gtaatttctc cggggaaacc acgcgtaatc
    57181 cccacggtaa gtgattaatt tcgattattt ttcccggatg gaaaattctt agaaaccgat
    57241 cacatacagc tgcatttatt aaggttatca tccgtttcgc tgaaaaacat aacccataaa
    57301 atgctagctg taccaggaac cacctcctta gcctgtgtaa tctcccttac acgggcttat
    57361 tttttatgca taagccctat ccctggtcac cgtcttccat tgaccacatc gatagaatct
    57421 cccttcatag cacgatgcct ttcacgtaac ggcatcgtgc tcgcacaggt tccggctaag
    57481 cacaaccaga acgcgcatgt ttgacgctta ccaaaaaata ttctcactct ccacatttga
    57541 atgtcagacg agcgacgcca tgtaatcctg caccttctgt cttcaggtca actatctgca
    57601 tttttttgcc ctgagtaaca cagaaatggg ctgcatcatt ttttactata ttttctgcac
    57661 cagatattct gcccctggct aaagaagctt cggcttcggt gtagtattgg ttatcgagtt
    57721 tacgctgaat attactttta tatgcaagac caaatttacc gatacttgtc tcatcattat
    57781 gcacagcaca accagacata ataaaaatac taattaatga tatagcagct atctttttca
    57841 tctcaccttc c
//
LOCUS       DAZFWL020000017        53205 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFWL020000017 DAZFWL020000000
VERSION     DAZFWL020000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 53205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.017"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            28..822
                     /gene="yjfZ"
                     /locus_tag="ACHKNP_RS05620"
                     /old_locus_tag="pgaptmp_001124"
     CDS             28..822
                     /gene="yjfZ"
                     /locus_tag="ACHKNP_RS05620"
                     /old_locus_tag="pgaptmp_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU4703333.1"
                     /translation="MTLPTTIYSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHKATLDNIHAQKY
                     NKAHAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(893..1342)
                     /gene="rplI"
                     /locus_tag="ACHKNP_RS05625"
                     /old_locus_tag="pgaptmp_001125"
     CDS             complement(893..1342)
                     /gene="rplI"
                     /locus_tag="ACHKNP_RS05625"
                     /old_locus_tag="pgaptmp_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGU4703334.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1384..1611)
                     /gene="rpsR"
                     /locus_tag="ACHKNP_RS05630"
                     /old_locus_tag="pgaptmp_001126"
     CDS             complement(1384..1611)
                     /gene="rpsR"
                     /locus_tag="ACHKNP_RS05630"
                     /old_locus_tag="pgaptmp_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGU4703335.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1616..1930)
                     /gene="priB"
                     /locus_tag="ACHKNP_RS05635"
                     /old_locus_tag="pgaptmp_001127"
     CDS             complement(1616..1930)
                     /gene="priB"
                     /locus_tag="ACHKNP_RS05635"
                     /old_locus_tag="pgaptmp_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HGU4703336.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1937..2332)
                     /gene="rpsF"
                     /locus_tag="ACHKNP_RS05640"
                     /old_locus_tag="pgaptmp_001128"
     CDS             complement(1937..2332)
                     /gene="rpsF"
                     /locus_tag="ACHKNP_RS05640"
                     /old_locus_tag="pgaptmp_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGU4703337.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2659..2934
                     /gene="yjfY"
                     /locus_tag="ACHKNP_RS05645"
                     /old_locus_tag="pgaptmp_001129"
     CDS             2659..2934
                     /gene="yjfY"
                     /locus_tag="ACHKNP_RS05645"
                     /old_locus_tag="pgaptmp_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HGU4703338.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(3063..3749)
                     /gene="ulaF"
                     /locus_tag="ACHKNP_RS05650"
                     /old_locus_tag="pgaptmp_001130"
     CDS             complement(3063..3749)
                     /gene="ulaF"
                     /locus_tag="ACHKNP_RS05650"
                     /old_locus_tag="pgaptmp_001130"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HGU4703339.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3749..4603)
                     /gene="ulaE"
                     /locus_tag="ACHKNP_RS05655"
                     /old_locus_tag="pgaptmp_001131"
     CDS             complement(3749..4603)
                     /gene="ulaE"
                     /locus_tag="ACHKNP_RS05655"
                     /old_locus_tag="pgaptmp_001131"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HGU4703340.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(4613..5263)
                     /gene="ulaD"
                     /locus_tag="ACHKNP_RS05660"
                     /old_locus_tag="pgaptmp_001132"
     CDS             complement(4613..5263)
                     /gene="ulaD"
                     /locus_tag="ACHKNP_RS05660"
                     /old_locus_tag="pgaptmp_001132"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGU4703341.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5277..5741)
                     /gene="ulaC"
                     /locus_tag="ACHKNP_RS05665"
                     /old_locus_tag="pgaptmp_001133"
     CDS             complement(5277..5741)
                     /gene="ulaC"
                     /locus_tag="ACHKNP_RS05665"
                     /old_locus_tag="pgaptmp_001133"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HGU4703342.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(5751..6056)
                     /gene="ulaB"
                     /locus_tag="ACHKNP_RS05670"
                     /old_locus_tag="pgaptmp_001134"
     CDS             complement(5751..6056)
                     /gene="ulaB"
                     /locus_tag="ACHKNP_RS05670"
                     /old_locus_tag="pgaptmp_001134"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HGU4703343.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6072..7469)
                     /gene="ulaA"
                     /locus_tag="ACHKNP_RS05675"
                     /old_locus_tag="pgaptmp_001135"
     CDS             complement(6072..7469)
                     /gene="ulaA"
                     /locus_tag="ACHKNP_RS05675"
                     /old_locus_tag="pgaptmp_001135"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGU4703344.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGYIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            7824..8888
                     /gene="ulaG"
                     /locus_tag="ACHKNP_RS05680"
                     /old_locus_tag="pgaptmp_001136"
     CDS             7824..8888
                     /gene="ulaG"
                     /locus_tag="ACHKNP_RS05680"
                     /old_locus_tag="pgaptmp_001136"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGU4703345.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8996..9751
                     /gene="ulaR"
                     /locus_tag="ACHKNP_RS05685"
                     /old_locus_tag="pgaptmp_001137"
     CDS             8996..9751
                     /gene="ulaR"
                     /locus_tag="ACHKNP_RS05685"
                     /old_locus_tag="pgaptmp_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HGU4703346.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(9748..10497)
                     /gene="yjfP"
                     /locus_tag="ACHKNP_RS05690"
                     /old_locus_tag="pgaptmp_001138"
     CDS             complement(9748..10497)
                     /gene="yjfP"
                     /locus_tag="ACHKNP_RS05690"
                     /old_locus_tag="pgaptmp_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4703347.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITTRHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            10679..11008
                     /gene="bsmA"
                     /locus_tag="ACHKNP_RS05695"
                     /old_locus_tag="pgaptmp_001139"
     CDS             10679..11008
                     /gene="bsmA"
                     /locus_tag="ACHKNP_RS05695"
                     /old_locus_tag="pgaptmp_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HGU4703348.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11157..11432
                     /gene="yjfN"
                     /locus_tag="ACHKNP_RS05700"
                     /old_locus_tag="pgaptmp_001140"
     CDS             11157..11432
                     /gene="yjfN"
                     /locus_tag="ACHKNP_RS05700"
                     /old_locus_tag="pgaptmp_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HGU4703349.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11549..13174)
                     /gene="aidB"
                     /locus_tag="ACHKNP_RS05705"
                     /old_locus_tag="pgaptmp_001141"
     CDS             complement(11549..13174)
                     /gene="aidB"
                     /locus_tag="ACHKNP_RS05705"
                     /old_locus_tag="pgaptmp_001141"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HGU4703350.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13258..14421)
                     /gene="yjfC"
                     /locus_tag="ACHKNP_RS05710"
                     /old_locus_tag="pgaptmp_001142"
     CDS             complement(13258..14421)
                     /gene="yjfC"
                     /locus_tag="ACHKNP_RS05710"
                     /old_locus_tag="pgaptmp_001142"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU4703351.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(14424..15062)
                     /gene="yjfM"
                     /locus_tag="ACHKNP_RS05715"
                     /old_locus_tag="pgaptmp_001143"
     CDS             complement(14424..15062)
                     /gene="yjfM"
                     /locus_tag="ACHKNP_RS05715"
                     /old_locus_tag="pgaptmp_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HGU4703352.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15072..15470)
                     /gene="yjfL"
                     /locus_tag="ACHKNP_RS05720"
                     /old_locus_tag="pgaptmp_001144"
     CDS             complement(15072..15470)
                     /gene="yjfL"
                     /locus_tag="ACHKNP_RS05720"
                     /old_locus_tag="pgaptmp_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HGU4703353.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15488..16147)
                     /gene="yjfK"
                     /locus_tag="ACHKNP_RS05725"
                     /old_locus_tag="pgaptmp_001145"
     CDS             complement(15488..16147)
                     /gene="yjfK"
                     /locus_tag="ACHKNP_RS05725"
                     /old_locus_tag="pgaptmp_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HGU4703354.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16198..16896)
                     /gene="yjfJ"
                     /locus_tag="ACHKNP_RS05730"
                     /old_locus_tag="pgaptmp_001146"
     CDS             complement(16198..16896)
                     /gene="yjfJ"
                     /locus_tag="ACHKNP_RS05730"
                     /old_locus_tag="pgaptmp_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HGU4703355.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(16915..17316)
                     /gene="yjfI"
                     /locus_tag="ACHKNP_RS05735"
                     /old_locus_tag="pgaptmp_001147"
     CDS             complement(16915..17316)
                     /gene="yjfI"
                     /locus_tag="ACHKNP_RS05735"
                     /old_locus_tag="pgaptmp_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HGU4703356.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(17443..18174)
                     /gene="rlmB"
                     /locus_tag="ACHKNP_RS05740"
                     /old_locus_tag="pgaptmp_001148"
     CDS             complement(17443..18174)
                     /gene="rlmB"
                     /locus_tag="ACHKNP_RS05740"
                     /old_locus_tag="pgaptmp_001148"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGU4703357.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(18354..20795)
                     /gene="rnr"
                     /locus_tag="ACHKNP_RS05745"
                     /old_locus_tag="pgaptmp_001149"
     CDS             complement(18354..20795)
                     /gene="rnr"
                     /locus_tag="ACHKNP_RS05745"
                     /old_locus_tag="pgaptmp_001149"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGU4703358.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRSGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(20834..21259)
                     /gene="nsrR"
                     /locus_tag="ACHKNP_RS05750"
                     /old_locus_tag="pgaptmp_001150"
     CDS             complement(20834..21259)
                     /gene="nsrR"
                     /locus_tag="ACHKNP_RS05750"
                     /old_locus_tag="pgaptmp_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HGU4703359.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(21464..22762)
                     /gene="purA"
                     /locus_tag="ACHKNP_RS05755"
                     /old_locus_tag="pgaptmp_001151"
     CDS             complement(21464..22762)
                     /gene="purA"
                     /locus_tag="ACHKNP_RS05755"
                     /old_locus_tag="pgaptmp_001151"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGU4703360.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(22866..23063)
                     /gene="yjeT"
                     /locus_tag="ACHKNP_RS05760"
                     /old_locus_tag="pgaptmp_001152"
     CDS             complement(22866..23063)
                     /gene="yjeT"
                     /locus_tag="ACHKNP_RS05760"
                     /old_locus_tag="pgaptmp_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HGU4703361.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(23145..24149)
                     /gene="hflC"
                     /locus_tag="ACHKNP_RS05765"
                     /old_locus_tag="pgaptmp_001153"
     CDS             complement(23145..24149)
                     /gene="hflC"
                     /locus_tag="ACHKNP_RS05765"
                     /old_locus_tag="pgaptmp_001153"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HGU4703362.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(24152..25411)
                     /gene="hflK"
                     /locus_tag="ACHKNP_RS05770"
                     /old_locus_tag="pgaptmp_001154"
     CDS             complement(24152..25411)
                     /gene="hflK"
                     /locus_tag="ACHKNP_RS05770"
                     /old_locus_tag="pgaptmp_001154"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HGU4703363.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(25497..26777)
                     /gene="hflX"
                     /locus_tag="ACHKNP_RS05775"
                     /old_locus_tag="pgaptmp_001155"
     CDS             complement(25497..26777)
                     /gene="hflX"
                     /locus_tag="ACHKNP_RS05775"
                     /old_locus_tag="pgaptmp_001155"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HGU4703364.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTGRLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(26854..27162)
                     /gene="hfq"
                     /locus_tag="ACHKNP_RS05780"
                     /old_locus_tag="pgaptmp_001156"
     CDS             complement(26854..27162)
                     /gene="hfq"
                     /locus_tag="ACHKNP_RS05780"
                     /old_locus_tag="pgaptmp_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HGU4703365.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(27248..28198)
                     /gene="miaA"
                     /locus_tag="ACHKNP_RS05785"
                     /old_locus_tag="pgaptmp_001157"
     CDS             complement(27248..28198)
                     /gene="miaA"
                     /locus_tag="ACHKNP_RS05785"
                     /old_locus_tag="pgaptmp_001157"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGU4703366.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAVEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(28191..30038)
                     /gene="mutL"
                     /locus_tag="ACHKNP_RS05790"
                     /old_locus_tag="pgaptmp_001158"
     CDS             complement(28191..30038)
                     /gene="mutL"
                     /locus_tag="ACHKNP_RS05790"
                     /old_locus_tag="pgaptmp_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGU4703367.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(30048..31385)
                     /gene="amiB"
                     /locus_tag="ACHKNP_RS05795"
                     /old_locus_tag="pgaptmp_001159"
     CDS             complement(30048..31385)
                     /gene="amiB"
                     /locus_tag="ACHKNP_RS05795"
                     /old_locus_tag="pgaptmp_001159"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HGU4703368.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(31404..31865)
                     /gene="tsaE"
                     /locus_tag="ACHKNP_RS05800"
                     /old_locus_tag="pgaptmp_001160"
     CDS             complement(31404..31865)
                     /gene="tsaE"
                     /locus_tag="ACHKNP_RS05800"
                     /old_locus_tag="pgaptmp_001160"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGU4703369.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(31837..33384)
                     /gene="nnr"
                     /locus_tag="ACHKNP_RS05805"
                     /old_locus_tag="pgaptmp_001161"
     CDS             complement(31837..33384)
                     /gene="nnr"
                     /locus_tag="ACHKNP_RS05805"
                     /old_locus_tag="pgaptmp_001161"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HGU4703370.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            33383..34522
                     /gene="queG"
                     /locus_tag="ACHKNP_RS05810"
                     /old_locus_tag="pgaptmp_001162"
     CDS             33383..34522
                     /gene="queG"
                     /locus_tag="ACHKNP_RS05810"
                     /old_locus_tag="pgaptmp_001162"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HGU4703371.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAAKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(34505..34558)
                     /locus_tag="ACHKNP_RS05815"
                     /old_locus_tag="pgaptmp_001163"
     CDS             complement(34505..34558)
                     /locus_tag="ACHKNP_RS05815"
                     /old_locus_tag="pgaptmp_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703372.1"
                     /translation="MRFYFYSQAVDESGVTW"
     gene            complement(34792..34867)
                     /locus_tag="ACHKNP_RS05820"
                     /old_locus_tag="pgaptmp_001164"
     tRNA            complement(34792..34867)
                     /locus_tag="ACHKNP_RS05820"
                     /old_locus_tag="pgaptmp_001164"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34832..34834),aa:Gly,seq:gcc)
     gene            complement(34903..34978)
                     /locus_tag="ACHKNP_RS05825"
                     /old_locus_tag="pgaptmp_001165"
     tRNA            complement(34903..34978)
                     /locus_tag="ACHKNP_RS05825"
                     /old_locus_tag="pgaptmp_001165"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34943..34945),aa:Gly,seq:gcc)
     gene            complement(35136..35211)
                     /locus_tag="ACHKNP_RS05830"
                     /old_locus_tag="pgaptmp_001166"
     tRNA            complement(35136..35211)
                     /locus_tag="ACHKNP_RS05830"
                     /old_locus_tag="pgaptmp_001166"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35176..35178),aa:Gly,seq:gcc)
     gene            complement(35422..35967)
                     /gene="orn"
                     /locus_tag="ACHKNP_RS05835"
                     /old_locus_tag="pgaptmp_001167"
     CDS             complement(35422..35967)
                     /gene="orn"
                     /locus_tag="ACHKNP_RS05835"
                     /old_locus_tag="pgaptmp_001167"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HGU4703373.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            36062..37114
                     /gene="rsgA"
                     /locus_tag="ACHKNP_RS05840"
                     /old_locus_tag="pgaptmp_001168"
     CDS             36062..37114
                     /gene="rsgA"
                     /locus_tag="ACHKNP_RS05840"
                     /old_locus_tag="pgaptmp_001168"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HGU4703374.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            37211..38179
                     /gene="asd"
                     /locus_tag="ACHKNP_RS05845"
                     /old_locus_tag="pgaptmp_001169"
     CDS             37211..38179
                     /gene="asd"
                     /locus_tag="ACHKNP_RS05845"
                     /old_locus_tag="pgaptmp_001169"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HGU4703375.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            38201..41524
                     /gene="mscM"
                     /locus_tag="ACHKNP_RS05850"
                     /old_locus_tag="pgaptmp_001170"
     CDS             38201..41524
                     /gene="mscM"
                     /locus_tag="ACHKNP_RS05850"
                     /old_locus_tag="pgaptmp_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HGU4703376.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(41553..41867)
                     /gene="yjeO"
                     /locus_tag="ACHKNP_RS05855"
                     /old_locus_tag="pgaptmp_001171"
     CDS             complement(41553..41867)
                     /gene="yjeO"
                     /locus_tag="ACHKNP_RS05855"
                     /old_locus_tag="pgaptmp_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HGU4703377.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(41864..42178)
                     /gene="yjeN"
                     /locus_tag="ACHKNP_RS05860"
                     /old_locus_tag="pgaptmp_001172"
     CDS             complement(41864..42178)
                     /gene="yjeN"
                     /locus_tag="ACHKNP_RS05860"
                     /old_locus_tag="pgaptmp_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HGU4703378.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(42230..43732)
                     /gene="yjeM"
                     /locus_tag="ACHKNP_RS05865"
                     /old_locus_tag="pgaptmp_001173"
     CDS             complement(42230..43732)
                     /gene="yjeM"
                     /locus_tag="ACHKNP_RS05865"
                     /old_locus_tag="pgaptmp_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HGU4703379.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(43951..44928)
                     /gene="epmA"
                     /locus_tag="ACHKNP_RS05870"
                     /old_locus_tag="pgaptmp_001174"
     CDS             complement(43951..44928)
                     /gene="epmA"
                     /locus_tag="ACHKNP_RS05870"
                     /old_locus_tag="pgaptmp_001174"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGU4703380.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            45253..47061
                     /gene="frdA"
                     /locus_tag="ACHKNP_RS05875"
                     /old_locus_tag="pgaptmp_001175"
     CDS             45253..47061
                     /gene="frdA"
                     /locus_tag="ACHKNP_RS05875"
                     /old_locus_tag="pgaptmp_001175"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGU4703381.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            47054..47788
                     /gene="frdB"
                     /locus_tag="ACHKNP_RS05880"
                     /old_locus_tag="pgaptmp_001176"
     CDS             47054..47788
                     /gene="frdB"
                     /locus_tag="ACHKNP_RS05880"
                     /old_locus_tag="pgaptmp_001176"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGU4703382.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            47799..48194
                     /gene="frdC"
                     /locus_tag="ACHKNP_RS05885"
                     /old_locus_tag="pgaptmp_001177"
     CDS             47799..48194
                     /gene="frdC"
                     /locus_tag="ACHKNP_RS05885"
                     /old_locus_tag="pgaptmp_001177"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGU4703383.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            48205..48564
                     /gene="frdD"
                     /locus_tag="ACHKNP_RS05890"
                     /old_locus_tag="pgaptmp_001178"
     CDS             48205..48564
                     /gene="frdD"
                     /locus_tag="ACHKNP_RS05890"
                     /old_locus_tag="pgaptmp_001178"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU4703384.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            48627..49760
                     /gene="ampC"
                     /locus_tag="ACHKNP_RS05895"
                     /old_locus_tag="pgaptmp_001179"
     CDS             48627..49760
                     /gene="ampC"
                     /locus_tag="ACHKNP_RS05895"
                     /old_locus_tag="pgaptmp_001179"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU4703385.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            49849..50382
                     /gene="blc"
                     /locus_tag="ACHKNP_RS05900"
                     /old_locus_tag="pgaptmp_001180"
     CDS             49849..50382
                     /gene="blc"
                     /locus_tag="ACHKNP_RS05900"
                     /old_locus_tag="pgaptmp_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU4703386.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(50379..50696)
                     /gene="sugE"
                     /locus_tag="ACHKNP_RS05905"
                     /old_locus_tag="pgaptmp_001181"
     CDS             complement(50379..50696)
                     /gene="sugE"
                     /locus_tag="ACHKNP_RS05905"
                     /old_locus_tag="pgaptmp_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU4703387.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(50878..51024)
                     /gene="ecnB"
                     /locus_tag="ACHKNP_RS05910"
                     /old_locus_tag="pgaptmp_001182"
     CDS             complement(50878..51024)
                     /gene="ecnB"
                     /locus_tag="ACHKNP_RS05910"
                     /old_locus_tag="pgaptmp_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU4703388.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(51135..51260)
                     /gene="ecnA"
                     /locus_tag="ACHKNP_RS05915"
                     /old_locus_tag="pgaptmp_001183"
     CDS             complement(51135..51260)
                     /gene="ecnA"
                     /locus_tag="ACHKNP_RS05915"
                     /old_locus_tag="pgaptmp_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGU4703389.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(51312..51878)
                     /gene="efp"
                     /locus_tag="ACHKNP_RS05920"
                     /old_locus_tag="pgaptmp_001184"
     CDS             complement(51312..51878)
                     /gene="efp"
                     /locus_tag="ACHKNP_RS05920"
                     /old_locus_tag="pgaptmp_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGU4703390.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            51920..52948
                     /gene="epmB"
                     /locus_tag="ACHKNP_RS05925"
                     /old_locus_tag="pgaptmp_001185"
     CDS             51920..52948
                     /gene="epmB"
                     /locus_tag="ACHKNP_RS05925"
                     /old_locus_tag="pgaptmp_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGU4703391.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT        12958 a        14480 c        13241 g        12526 t
ORIGIN      
        1 taaaaacatt aaacaggtaa cattacaatg acattaccaa cgactattta ttcatttcct
       61 gcatattcac cccatttttc gtccaccgac aaaccagtaa aattaaaatt tcatcaatat
      121 gcacgtgcta cattactgcc aaatcgtgga agagatcata attgcgatgg cagacgtact
      181 gttgagatcc acaaactaga tctctctgac tggcaagcat tcaataagtt agcaacgaag
      241 tgtaatgctt acgatggagt aacaatgaat ggtgataatt cttttggatg gaaccataaa
      301 gctacattag ataatattca cgcccagaaa tacaataaag ctcacgcggg tgcgaggtta
      361 accgcagaac tgaaatattt attgcaagat gtcgaatcct tcgaacccaa cagtaaatac
      421 acaatacacg aagtggtttt gggacctggt tatggtactc cagactatac cggccaaacg
      481 ataggatacg ttgtaaccct tcctgcccaa atgcctaatt gctggtcatc tgaactacca
      541 acaatcgatc tttatatcga ccaacttcgc actgttacag gcgtttctaa tgccctcgga
      601 ttcatcattg ctgcattgct aaacgcgtat tctgatttac cccatgattt gaaaattggt
      661 ctacgttcct tatcaaattc agcagcaatc tactcgggtt taggtttcga gcgagtgccg
      721 caggaccgag acataaggag tgatcgtatg catctaacgc cagcaaacca tcctgactta
      781 tggacacaag agaatggtga atggatctac ttgcgaaact aatacctgac gaaaaataga
      841 aagcaaaacg ccgaccaatg gtcggcgttt ttacgtctcg ttgaataacg aattattcag
      901 ctactacgtt tacgatcact ttcgcgaata cttcgctgtg aacctggaag ctcacttcgt
      961 gttcgccagt ggtacgcaga acgccgttcg gcagacgaac ttcgctctta gccacttcaa
     1021 cgccagctgc agttacagcg tcagcgatgt cgcgagtacc gatggaaccg aacagtttac
     1081 cttcgtcgcc agctttagac gcgatggtaa cagtttccag tgcattgatt ttctcagcgc
     1141 gagcattagc agctgccaga acttcagcca gtttagcttc cagttcagcg cgacgtgctt
     1201 cgaagaattc aatgtttttc ttggtagctg gaacagcttt accctgcggt accaggaagt
     1261 tacgagcata gcccgcttta acgtttacct gatcacccag gctacccagg tttgctactt
     1321 tatcaagcag aataacttgc attaccttat cctctcaaag tcgtattaat ggaccgtgac
     1381 cgattactga tggcgatcag tgtacggcag cagggacagg tagcgagcgc gtttgatagc
     1441 gcgagccagc tgacgctggt attttgcacg ggtaccggtg atacggcttg ggacaatctt
     1501 accgctttcg gtgatgtagt ttttcagcgt agcgatatct ttatagtcga tctcttgaac
     1561 gccttccgcg gtgaaacggc agaacttgcg acgacggaaa taacgtgcca tatggctagt
     1621 ctccagaatc tatcaattca atctgctcgg catgcaaaac cattttgctc agtccgttct
     1681 ttgccttgtg gcatgaaatg aacccctgaa cggttatgcg actgccgacc gttatactgt
     1741 gagtaatggc ctggttttcg tgtccgctaa caataacggg catttgacac catgcctgcc
     1801 ggtgaaagcc ggcttcctcc tgcacagaac gatgctcaag cacgaactgg cagtgaggaa
     1861 ttcctgatgg actgaccttt cgaaggggag tcctgcacac ggtgccggac aacaccagac
     1921 ggttggtcat cagaaattac tcttcagaat ccccagcttc agcatcatca gcggtttcgt
     1981 ttgcgaaatc atcgcgacgc tcacggcgct cgtctttcgc tttaaccatc ggagatgctt
     2041 cggtaacagc gtgcttggta cgcataacca tgctgcggat aacggcatcg ttgaagcgga
     2101 aggtagtttc cagctcatcg atcacttcct gcggagcttc aacgttcatc agaacgtagt
     2161 gtgctttgtg cagtttgttg atcgggtaag ccagctgacg gcggccccag tcttccagac
     2221 ggtggatctt gccttctgca ccagtgatgg cagcagtgta gcgctcgatc atgcccggaa
     2281 cctgttcgct ctggtcagga tggaccataa aaacgatttc gtaatgacgc atcgaattgc
     2341 tccttacgga ttattcagcc tcctgtctgg gtcagccgaa tcccggggag gcaaggaacg
     2401 tgttaaaggt cggctgaaaa atgacgcgtt atagtacttg tatgccccgc taaactcaag
     2461 gccagccgac aaataaatct catctttcgc tcaaaagaac taactccctg atttgaagtt
     2521 catcacattc cgtcacagtg ttttttcgaa caactgcatc agaaatggtc accatcaact
     2581 tctttatcat tgattaaaat taaatcagaa gatcatcaca gaggagagca cacaaagtca
     2641 gccgttgagg aggtatctat gttcagtcgt gttttagccc ttctggctgt gcttttgcta
     2701 agtgcaaata catgggcagc cattgaaatc aataaccacc aggccagaaa tatggacgat
     2761 gtgcaaagct taggcgtgat ttatatcaat cataatttcg ccactgaaag tgaagcacgt
     2821 caggcattaa atgaagaaac agatgcgcag ggcgcaacgt actaccacgt aattctgatg
     2881 cgggaaccgg ggagtaacgg caatatgcac gccagcgcgg atatttatcg ctagcaccag
     2941 gtataaccaa cgaaacattg ccatagtttg ctttgccccc ttcgcagggg gctttttttg
     3001 aaaaacggct gcggaattag accagttatc tcccgaagaa ggaaatttcc gcagcgcgtg
     3061 ttttacttct gcccgtaata agcgttagga ccatgcttac gcatgaagtg tttattcatc
     3121 aggaagctgt cgatgtgatt gagttgtgga ttaatactgc gcgcaatcca cgccattttc
     3181 gccacttctt ccatcactac cgcgttatgc accgcgtcgt gggcatcttt cccccaggcg
     3241 aacggtccgt gctgatacac cacaattccc ggcgtatgca gcggctcggc gttgcccagc
     3301 gtttcgataa tcactttgcc agtgttcagt tcatactcac cctgcacctc ttcttcgctt
     3361 aatccacgcg tacacgggat gtcaccaaag aagtagtcag cgtgcgtggt gcctaacgcc
     3421 gggatcgcca gccccgcctg cgcccatgcg gtagcatgag tggagtgggt atggacaatg
     3481 ccgccaagcg acggataacg acggtagagt tcaagatgcg tcgcggtgtc ggaagatggg
     3541 cgatactccc cttccaccac cttaccgctc atatcgacca ccaccatatc gtctgctttc
     3601 atggtttcgt aggcgacgcc gctgggcttg atcaccacca gcccgcgttc gcggtcgata
     3661 gcgctgacgt tgccccaggt aaaggtcacc agcccgtagc gcggcagatc catgttggct
     3721 tcaaatacct gctgttttag cttttgcatt atgccgcctc caccatgccc gctttcgcca
     3781 tgcgcgcttt cacccaatca cgcgctttcg ccacttctgc cgccgggtct tccgccgttt
     3841 cgctccacat ctcaatcagg tacggcccgc aatagccact ctgtttgagc gtttcgaaac
     3901 aacgttcgaa atccactaca ccttcgccaa acggcacgtt tttgaagacg ccaggtttgg
     3961 tgtctttcac atgtaccgcg acgatatgcc cgattccggc ctgcaactcc atctgcacat
     4021 cgttgtccca cgccgacagg ttgccgatat ccgggtagag ctggaaccac gggttgttga
     4081 gatagtgcgc gtaacccagc gccttgctga tggagttcat caacggataa tccatgatct
     4141 ccatcgccag cgtcacctgc gcgcggctcg ccatctcaac gctctctttc aggccgtcac
     4201 ggaaacgacg acgcgtttcg ttattggctt cctgatagta aacgtcatag cccgccagct
     4261 ggatcacgcg aatgccgacg tcctgggcga actggatagc tttacgcata atctccagcc
     4321 cctgcgcccg caccgcgtca tcttcactgc ccagcgggaa acgacgatga gcagaaaggc
     4381 acatggacgg cacgcgcacg ccggtttcaa caatcgcatt caccagcgcc agacgctgct
     4441 cgcggctcca gtcgaggcgc gacaggcgct cgtccgtttc atctaccgac atctcgacaa
     4501 aatcgaagcc caacgttttt gccagttgca ggcgttccag ccagcactcc ccggcgggga
     4561 gcgctttttc atagatgcca agcgggattt gtttggacaa catatccgct ccttagcccc
     4621 acagttcggc gatggaacgt ttgaactgac gtgcagcttc aaccggagaa gcggcatcac
     4681 ggatgctgcg accagcgata aacacatgaa tcggaatacc tttgaacagc ggcagatctt
     4741 ccagcgccag gccaccggtg acggtgactt tgaagcccat atcggaaaga cgtttgatcg
     4801 cggtaatgtc cgcttcgccc cacgctacgc ctgcggcctg cgcgtcacgg ctgcggtgat
     4861 aaaccacctg ctgaatgcct gcgtcacgcc actgctgcgc ctgttcccag gtccagtagc
     4921 cggtcaattc gatctgcacg tcgccgttaa actcttttgc tacgtccagc gcgcctttgg
     4981 cggtgttgat atccgcacag caaattaccg tcacccagtc agcgttggct tcgaagcaca
     5041 tacgcgaaag gattttgccc gcatcagcaa ttttagcgtc tgccagtacg attttgtgcg
     5101 ggtagagcgc tttcaggtca cgaaccgcac gcacgccttc gcccacgcac agaatggtac
     5161 ccacttcgat aatgtcgact tcttcggcaa tcaggcgagt ggtttcgtag gcgctatcca
     5221 tagtctggtt gtccagcgcg acttgcaaca tcggtaatga catcttcaat tccttcttaa
     5281 gctgccgcgt tggtgcggtc aatgagatcc agtacttcct gctcggtacg gcaggcacgt
     5341 aaacggtcga aattttcttc atcttcaaac aggttgacga tctgcatgat gcccacttcc
     5401 tggtgcgtgt tggcatcgac cgccgccatg gtgatgagga tatccaccgg gtcgttatct
     5461 tcgtggttga actccagcgg ctttttcagc gtcaccagcg caaaaccggt tttcttaacg
     5521 ccctcttccg ggcgaccgtg cggcattgcc aggcctggtg cgagcacaaa gtacggaccg
     5581 tgctgttcaa ccgcatccag aatcgcctgg tagtaacgcg gctcgacaac atccgccgcc
     5641 accagcaggt caacgccgat ttttactgcg tcctgccatg tctcagcttc agcctgcagg
     5701 cggatggatt tattctccgc cagcgaatca cgtaatttca tggcgcgtcc ttacttcaca
     5761 tcctgcggga aatgcgcttt gatcacttcc agcagtttcg ggccaaagtc ggcaggagag
     5821 agcatgttgc gcacgccaac cacgtatttg ttgccggtca cggtgatttc gcccgcaatg
     5881 tgcgtagaag cgatgatgat atccgcgccg ctcaactcgc ttttgtattc gccaaccgcg
     5941 cagctattta ccgtgtggtc aatgtttgat tgggttaaaa actggtccac tttcatcttc
     6001 atgatcatgg aactgccttg tccgttgcca cacacagcca gaatacgtac ggtcataatc
     6061 aaaactcctt attaagcaga ctgttctgcc agttgttttt ctgcatcttc ttctgcgcgc
     6121 agtgcgcggc cagcgaagaa catataagcc agtgcaatta caatgatgac ggccataaag
     6181 gcgataccga tggagaagaa gccctgcatc atcggcggtg ccagaatcga ccagtccgcc
     6241 atgcccatcc aggcgctcat accggtgagt ttcaccgccc agacgcagcc aaagatttca
     6301 atcatcccca tcaccagaca tatcttcagc gccgcacgcc agccgccgaa gtggttagcg
     6361 aacacgccga tggtggcgtt ggagaagaac atcgggataa agccgggaat aatcaggatc
     6421 gaggaaccgc aggcgaccag gatgccaacc gcaatcagct gaccgatggt gccccacata
     6481 aagccccaaa ccacggcgtt cggcgcgaag ctatagatag ccgcacagtc aatcgccaga
     6541 accgcaccag ggatcaggcg ctgggagata ccgttaaatg cttcagagag ttccgccaca
     6601 aacatgcgca cgccctgagt gatgatgaag atcgccaccg cgaaggagaa accagtttgc
     6661 aggatgtaca ccgtccagtt cactttgcct gccatcgcct gcacggtgtc gataccgaag
     6721 gagagcagaa tggcaccaaa gaagatggtc atcacaatcg ccgtggagac gatgttgtcg
     6781 tggaaaatat tcagccagcc tggcagtttg aggtcttcaa cgctttcctc ttttttgccg
     6841 aggaacggcg caactttata ggcaatccat gatgcaaact gttgctggtg accgatggag
     6901 aaaccgcagc cgtccgtcac ttcctgagtc ggcttgtaca tcatgttgga ggtaatgccc
     6961 cagtagagcg aaaccagaat cgccgtgcag ataatggttg tccacatgga gtagccgaaa
     7021 ataaacaacg taacggcaat cagccccgcc tgctggaaca tgatataacc agtcagcatg
     7081 atggtacgaa taccggtaat gcgacgcagc agcacgtaac agatgttcag cgccagcgct
     7141 aacaacaccg cgtaacctac ccagctatag gcatcgccca tgcggtcgat ggttgccatc
     7201 atcgacgcgt aagtatcgga aattgcgccg ttaatgccgt agacttcgga cattttcgcc
     7261 accaccggtt tgaaggtgct ggtgaggatg ccggaccctg cctgcaacaa catgaaacca
     7321 attatggttt taatcgtgcc tttaataata acgctgacac ttttgcgcag taggatgtag
     7381 cccagacagg tcacaatacc cagcaacaac ggggcattgg tcatgacctg gttaaaaaac
     7441 acggtaaaga tgttgtagag gatctccata acgatctcct gaagaagagg taaccgggta
     7501 ttccgcacac ccggaatgtt gtgcactcac tctaattttc aaaagtaatc acaacaagat
     7561 tatttatgat taaatgtgac gcgacccgca aataattcca cacggataga gtgggattca
     7621 cacaacgaaa caattattta tttaaattca tttagttaat ttaactacac attccattaa
     7681 atctttccac actcaaaatc aacaacaact tccctggcaa aaagtcaaaa aatggtcatt
     7741 gccttcctga aaaaatagtg tcaggattaa tcaaaaccaa tcacatattg attcgaattg
     7801 aactatcatg aaggtaaatg gcgatgagta aagtgaaaag tatcacccga gaatcctgga
     7861 tcctgagcac tttcccggag tggggtagct ggctgaatga agaaattgaa caagagcagg
     7921 tcgcccctgg cacatttgcg atgtggtggc taggctgcac cgggatctgg ttgaaatcag
     7981 aaggtggcac caacgtttgc gttgatttct ggtgcggcac tggcaaacaa agtcacggta
     8041 acccgttaat gaaacagggt caccagatgc agcgcatggc tggcgtgaaa aaactgcagc
     8101 caaacctacg caccaccccg tttgttcttg atccgtttgc gattcgccag atcgacgcgg
     8161 tactggcgac tcacgatcac aacgatcata tcgacgttaa cgtcgctgct gccgtgatgc
     8221 agaactgtgc tgatgacgta ccattcattg gaccgaaaac ctgcgttgat ttgtggattg
     8281 gctggggcgt accgaaagag cgttgcatcg tggtcaaacc gggcgatgtt gtaaaagtaa
     8341 aagacattga aattcatgcg cttgacgctt tcgaccgtac tgcactgatc accctgcctg
     8401 ccgatcaaaa agcggctggc gtactgccgg acggcatgga cgatcgcgcg gtgaactatc
     8461 tgttcaaaac gcctggcggc tccctgtatc acagcggcga ctcccactac tctaactatt
     8521 atgcgaagca cggtaacgaa catcagatcg atgtggcgtt aggatcgtat ggtgagaacc
     8581 cgcgcggtat taccgacaaa atgaccagcg ccgatatgct gcgtatgggt gaagccctga
     8641 atgcgaaagt ggttatcccg ttccaccacg acatctggtc aaacttccag gccgatccgc
     8701 aagagatccg cgtgctgtgg gagatgaaaa aagatcgtct gaagtatggc ttcaagccgt
     8761 ttatctggca ggttggcggc aaatttacct ggccgctgga taaagacaac ttcgagtacc
     8821 actatccgcg cggtttcgat gattgcttca ctattgaacc ggatctgccg ttcaagtcat
     8881 tcctgtaatc tgtcgtttat accggatagc gtggagaagt tcgcgctatc cggcgtagta
     8941 tttcctggct atttcaaata tcatctaaaa aaatcaaatt ttatcggaat agctcatgac
     9001 tgaagcacaa agacatcaaa tcctcctgga aatgctcgca caattgggct ttgtgaccgt
     9061 tgagaaagtc gttgagcgtc tgggaatttc gcctgccact gcgcgacgcg atatcaataa
     9121 acttgacgaa agcggcaaac tgaaaaaagt gcgcaatggc gcagaagcta ttacccagca
     9181 gcgcccgcgc tggacgccga tgaatctgca tcaggcgcag aatcacgatg aaaaagtacg
     9241 tatcgctaaa gcagcctcgc agctggttaa tccgggcgaa agcgtagtca tcaactgcgg
     9301 ctccaccgcg tttctgcttg ggcgggaaat gtgtggcaag ccagtgcaaa tcatcactaa
     9361 ttatctaccg ctggcaaatt acctgatcga tcaagaacat gacagcgtga tcattatggg
     9421 tggacagtac aacaaaagtc agtccatcac tttaagcccg cagggcagcg aaaacagtct
     9481 ctatgccggg cactggatgt ttaccagcgg aaaagggctg accgcagaag ggttgtataa
     9541 aaccgatatg ctgacagcaa tggcagagca gaagatgctg agcgtggtag ggaaactggt
     9601 ggtactggtt gatagcagta agattggcga acgcgcggga atgcttttta gccgtgccga
     9661 tcaaatcgat atgctgatca ccggcaaaaa tgctaacccg gaaatcctgc aacaactgga
     9721 agcgcaaggg gtcagcattc tgcgtgttta aagatgctgg cggaaaaatg tcacggcagc
     9781 atctaacgcc tcaggcgtaa tgcggtgacg cacgcctggc tgccatgaac aggttagcag
     9841 tttatcccgt cccgtctcgc ttaaggcctg ctgcaaacgt agtgattcgt cggcaggcac
     9901 aacgtcatcg tcgaggccat gccacagcag tagaggtctg tccccaagtt gttccaggtg
     9961 gtttgtcgct tcccactctg ccagcggcgc gataatgtta ttgaattcat tctgctgtgc
    10021 tgccgtttca ggtatcagcg gtggaaacag tgaacgggcg agcgatgtaa aatagcccga
    10081 tcccatcatg ctggcggtac atttcaccgt ggggtggcga gtggtaattc ccagtgccgt
    10141 catcgcgccc atcgacgcgc caccgactgc cagacggtca tcaagcagcc agttttcttc
    10201 ggctattgcc gcacgtaaag tagtgaattc ctgcatactt tgtagcaaga tttgccagaa
    10261 ttgatttaac cgccgcgctt cgtcgctact aaaacggcta ccgtgatcgg gcgcatccgg
    10321 catgatcacc cgcaaaccag cctgcgccag cgcaacggca aaatagctat acaccaggct
    10381 ggatgaagta aagccgtgat aaaaaattac gcacggtaac ggggtatctt tttgcccgac
    10441 aggataagca tgaagaacgg gaatatctgc cagctcgcgt gattctattt caatcatctg
    10501 tctctccttt ttgtctggta tgacatgaag aaatccttag cacaagtttc tgctggcggt
    10561 attagttcat aatgttgaga tgtgggttac gctttcgtca cgttttcatt cgaaaatcgc
    10621 gtaccaggta acaattcggg aacattcccc caaagttaaa ttaataaggc actacactat
    10681 ggttagcagg aaacgtaata gcgttattta ccggtttgcc agtttattat tggtgttgat
    10741 gttaagtgcc tgtagcgctc tgcaaggtac gccacagcca gcaccgccag taacgaacca
    10801 tccgcaagag attcgccgcg accagacgca agggttacag cgaataggta gcgtaagcac
    10861 gatggttcgg ggttctccgg atgacgcatt agcagaaatc agagcaaaag ctgtcgctgc
    10921 aaaagctgat tattacgttg tcgtcatggt ggacgaaacc atcgtgacag gacagtggta
    10981 ttcacaagcc attttgtacc gtaaataaat cagacaactt acatagtcag ctttacattg
    11041 ctttgcgtcc ctatgcgttt acctgtgcac aataaattac cggctggaag cctgggcgtt
    11101 tattaccgcg atggaatgcc ctgcaacatg tggggaaacg aaaaatggag ctgacgatga
    11161 aacaattact tgcctcaccc tcgctgcaat tagtgactta tcctgcgagc gccacggcac
    11221 agtctgcaga attcgccagt gctgactgcg tgacgggcct gaatgaaata ggtcagatct
    11281 cagttagcaa tatttcaggg gatccgcagg acgtggaacg tatcgtggcg ttaaaggccg
    11341 atgaacaagg tgcgtcatgg taccgcatta ttaccatgta cgaagaccag cagcctgata
    11401 actggcgcgt acaggcgata ctgtatgcat gagtattata tacagcaaat gaacaatgcc
    11461 ggatgcggcg cgagcgcctt gtccggccta caatgttcgc atatttcatt gagttaatcg
    11521 aaaccagcgt cgcatcagtc gtatacgctt acacacacac tccccccgtc gcccgcagca
    11581 ataaatcatt ctggatctgc tctgacaacc gtacgccgcc gcgcgtatct aacatcacct
    11641 gacaccacgc ctgcgccatt ggcggagagg catatttcaa catttgcgca ccgcagccca
    11701 gcaggaatag ctgatgagta atctctcgcc ccagttcttc agctggcttg cgcagctgct
    11761 gctgtaaacg acgaaccgcg cgatcaaaat agcgatcctg ccctttcact tccacaaacg
    11821 cttccgacaa taagtcgtat acgcccgctt gcttattgag aacgcgcagc acatccaggc
    11881 acataatatt gccggaacct tcccaaatac tgtttaccgg catctcccgg taaagccgcg
    11941 gcagctcgct ctcctcgcaa taaccaatgc cgcccagcac ctccatcgct tcggccacaa
    12001 acggcatacc tcttttgcag atcacaaatt ttgccgcagg cgtaaataaa cgcgcccaca
    12061 gggcttcttt ggcatcggca cgccggtccc acgctcgcgc aagacgaaac agcaacgccg
    12121 tttgcccttc aagctgaagt gccatgcgac ttaagacatg acgcataagg ggctgttgga
    12181 tcaatggatt accaaaaaca tggcgttgat gtgcatgata aatcgccagc gaaaatgcac
    12241 ggcgcatcat ggcatggcta cccagggcgc aatcaaaacg cgtcatccca cccattttca
    12301 ggatcagacg aattccttcc ccttcctgcc ccaacaacca gccaatggca tcctgaaact
    12361 ccacttcgca gctggcgtta gagcgattac ccagcttatc tttcagccgc tcgaggcgaa
    12421 tcgcgttgcg ttgcccgtca ggcaaaaagc gcggcacaaa aaagcaggac aaaccgcccg
    12481 tagtctgcgc cagcaccaga tgcgcatcgc tttgcggcac cgagaaaaac catttatgcc
    12541 ccaccagccg ataagagcca tcttccaggc gctctgcgcg ggtggtgttg ctcatgacat
    12601 cggaaccgcc ctgcttttcc gtcattccca tgccaatcaa caaaccgcgt ttttgcccac
    12661 ctggcaataa gtgagaatcg tagcgatcgc tcagcagcgg cgtggtccag tcctgaaacg
    12721 gcgtgggtaa catctgtaac aacaatggcg tggcggcaaa ggtcatcgtt attggacata
    12781 acgacccagc ctcaacctgc gcatgtaaca taaaacgcgc cgcgcgcgcc acaaatgcgc
    12841 cggagcgagc gtcttcttcc caggcaagat tgtgcacccg attggtacat aacgcctgca
    12901 tcagcaggtg ccaggcgggg tgaaaacgca catcgtccag acggcgtcct tgcgcatcgt
    12961 agcgcaataa ttcaggcgga ttcacattcg ccagccgccc cagttcaaga gattcagccg
    13021 ttcctaactg ctgaccaata ctggctagaa aatcgctatc ccagccagca ccttcacgcg
    13081 ttaccgcttc acagagcgcg ccatcagaca ggtataagtt gctgttattt aatggtatag
    13141 gttgattaaa aacggtgtga gtttgccagt gcactgtgtc tccctccatc aatggcagtc
    13201 acccttagta tggtcactgc catggattcc tgacagaaag agattgctaa aacattctta
    13261 tccagcaatg taatgcggaa cgaaacgtga ggtgtctttg gtgatcagcg tgttatcttc
    13321 acggatcccc attccgcacg cttcatcatc gacaatccag ctaccgatga gtgtgtagct
    13381 atcgccaaat cgcggcagag gttgaaacgc ctggtagatc atcggttcat cggcgtaatc
    13441 accatcagcg tggtcaacga cgttattctg accgtcgaaa atggtgacgt ttccgccttc
    13501 gcgcgagtag atcggtttac gcacatagct ttcgccagcg gcaatctgcg gtttttcgcc
    13561 ctcaaaccag gatgcaagaa ggttaggatg accagggaag aagcgccaaa gcagcggcat
    13621 tagcccttta ttactcaaga tacttttcca taacggctcc acccattgct cgcgacgctt
    13681 gcgcagcagc ggaccgttat cgtcacgcat catccactcc agcggataca gcttaaatgc
    13741 acgctggatg acattatcat caagatcggt cagtacaccg ccgacgccca aaccgagatc
    13801 ttcaatgtag ataaaccgcg actcctgccc tgcctgctgg gcgcagtcct gcaagtacag
    13861 cacggtagtc ctgtcttcat cggtgtcctg acagcagcag aaataaaacg gctcccggct
    13921 ataaagctcg ctaaagcgtg aaatcaggcg ttcctgaatg gcattgtact gatcggcatc
    13981 acgcggaata atgccgctgc gccgggcatc ctccagccac aaccactgga aataagccga
    14041 ctcatacaac gaggtcggcg tatcggcgtt gtactccagc agcttcaccg gcgcattgcc
    14101 acaccaggca aaatccatcc ggccatacag cgaaggatcg cgtgcgcgcc agctttcggc
    14161 gatcacatcc cagtacaacg gcgggatcgc cagttgcgtc aggatctctt cgtctttaac
    14221 cgcgcgatca accacctcaa ggcacatctg atgcagttcc gcagtcggtt tttcgatctg
    14281 ctcttcaatc tggcgcagag taaaacggta agcccgactc tcgtcccaat agatttcatt
    14341 gtcgatgata tgaaagtcga aaccgttgtt agcggcgatc tggtccagat cccgtcgcac
    14401 aggaacgttg tgtctcagca tgattagcct ccccaatgcc cacgggcgct ggaagaacga
    14461 ccatagccgc cgcgcgaaac ggttgacgct tttttggtgg taaagccgcc tgaagagtaa
    14521 gactcttttt tgccagaacc ggagcgccag gagtaatcac cggaagtgtt gcgccagacg
    14581 gggcgggaag caaatgagga tccgccgctg ttatagacga acggctcatc gcgatcttta
    14641 cgaataaccc ggcttaacaa aaatccagaa acgaccggga tccaactctg ttcaacattg
    14701 tcgtagtagc aattttcgta cttagactga caattctgct gagtcatatt cttcggaaca
    14761 tcggcataaa atgccgcttt ggcgttgttc cagccacgcg cgcaaatgtc ggcattatta
    14821 ccgtcatcaa tacaatcctg cactgtcgaa taaaacgttc cgtcgccgtc gttatcgaca
    14881 tcgccactat cgccacaacc tttcaataca aaaaaagcgg caccgcccat tagagccaac
    14941 gttaagtatt tttgtgcgta gcgattgcga cgcggttcaa aaggattatt cggtctacca
    15001 atgcgactga tcgctccgtg accaatttta ctgttatttc ttgatttgcg ttttctggcc
    15061 atgatcgtcc attaccatgt catacaagct gcgttaaaaa taccgccagc cagcgcggcg
    15121 gttcccataa acattcctgc tgcggtattg tgattaataa ttttttcgct taatgcgggc
    15181 atataaagtc tgacaccagc aaaaacgagt aactgaatca ccagtgcaat cccgccccag
    15241 gcgaaatagt ctggaatact caccgcattg attgccgcac tggataatgg gataacgtaa
    15301 cctaacaatg taccactgaa cgccagtgaa gccgcggtat tattgttttt gattaactgc
    15361 cattcgttgt gcggtgtaat tttagaatag ataaaaagaa aaataatcac catagccacg
    15421 ccaataaaaa aataggcgct aaaggcaaga agtgaatcca gtatatgcat tgtaaacgtc
    15481 cttgtaatta accaataata tgaagtgatg tcagcgggat atcgacaccc aacgcgcgcg
    15541 aaaataacca ctctggctcg ccgagatcgt taaaagattc ctcaccattt aacagcaaat
    15601 attcgtaagt atcttcggtt acttgccgct ggtagcccat ggtaaaatta tggacctccc
    15661 atttggcatg gttttgattt tctacttttt ccagcatgta gacgggttcg atatttcccg
    15721 gttcttcgct gttaaaaaat cgctgccagc gtttttcctg ccagtttaag atcattgccc
    15781 ccatcacttt ggcgttgatc gcctcgcgcc agtgactctc tttactgata ccgtagctct
    15841 cttcatagac aaagagcttg atatcatcaa tgtcatcaat atcttcgccg ccggtggtat
    15901 tgatttgcag aaattcatcg cctgaagtgt agtagcggaa aatctgacta ccgccgccca
    15961 gatcgatgtg gctgacagcg gctaccgtaa attcctcacc cggcagcgca atcagcaatt
    16021 catcttccag caaacgaaac gctaacgtgt cgagcgtaaa gccactattg agatgaagtc
    16081 ccagcggacc gcgagcgata gcaggcttat tatccttgcc aaacagacgc tggaaaaaac
    16141 cagacatatt ttatccttaa aatagttgcc agccttttag cgaggctggc aaaaaaatta
    16201 ctcgccctgt tggcgttgca gtcttgctaa tacatcctgg gcgctacttt tattgctacc
    16261 gccaattccg gcttccgcca gcttttcatc aagatcgcga ccgtctgcga ctttttccaa
    16321 ctgtgcagca gcatccaggc gagcctgacg ttcggcctga cgcgtttgca ggcgttttaa
    16381 ggattctgcc gctgtcgaaa cgctggaaga tgcgccaacg gtagaggttg ttacagcctg
    16441 ttgtgcacgc tgcatggctt cagtggcttt aacgacttcc atttgctgct caaactgagc
    16501 gatacgctgc gctgtcgctg taactgcttt ttccacgcca tcacgggaga cttccagatt
    16561 cgacaacact tgctcttcag cggtaatgag attctcaagg cgcgcgattt cttcagcaac
    16621 ttcgttaatc aacgacggat taacgttctt gcttaacgct tccagcgcac gggcttccag
    16681 actggctttg cgctcacgta aatctttcag cttatcgtga cttaatttca cccgcgccag
    16741 cagatcaacg cgagattttc cggctttatc gagttcagct ttagcgtcac gaatatgctg
    16801 ttccagcatg cgcacgccct gggtttcttc aatggattct tccgcctggg agataaacga
    16861 tttccccagc gtaaataaac tttttaaaat tcccataaat acccgtcctt gtaattagtg
    16921 tgaatattct tctgtaattt cagccagatc caatgcgtta tctaccagcg aggttatctc
    16981 aagcaggata tcttcaagag aagatttaag cgataacgcc ccgaaaacaa tgtaatactc
    17041 ttcctgttgc acgctggaga ttcctaccga tgacaacggc atcatcttct gatttcttaa
    17101 taagaaggta ttaaattcat ctggattgct gatactactt accggacaaa taaaggtttc
    17161 gataatcatt tggcgggaag taaaaagaat attgatttgc aaatcgccat aatcattcat
    17221 tttaataatt aatgcgctct cgctatttgt cacatcaata ttttgttcag gtacagtttg
    17281 cagcgccgtc gccagcgcca acggattcca tgtcatatat tattccatat agatttagtt
    17341 taaatattaa taaaatgaat atttgcagta cgtaattatc ttaccagcta tagacaaaaa
    17401 aaaaccatcc aaatctggat ggcttttcat aattcagacg aactagctgc gctgacgaac
    17461 cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc agggaagaaa cacttcctgc
    17521 cattgggatg ctgatcaact catcgcaatg ttcacgggtc aggcgacgca taccttcacc
    17581 ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg ctctgataga gtgtatgatc
    17641 cgcctcgcct gccgtaccga cgatccagat attctcttcc tgcaacatac gcatggtgcg
    17701 cgcaaggtta gtcacccgaa tcagtggaac gctttctgcc gcgccgcagg ctactttttt
    17761 cgccgtagcg ttgagctgtg cggagcgatc tttcggcaca atcaccgcgt gaacgccagc
    17821 agcgtccgcg ctacgcaggc acgcgccgag gttgtgcgga tcggttacac cgtcgaggat
    17881 cagcaggaac ggttgatcga gcgaagcgat cagatccggc agatcgtttt cctgatactg
    17941 acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg gcaccgtcgc ttttctcgtc
    18001 gagatattgg cggtttgcca actggataac cacgccctga gactcaaggg cgtgaatcag
    18061 cggtaacaga cgtttatctt cacggccttt taaaataaag acttcctgaa aacgttcagg
    18121 ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa atcatttcgc tcattaatgt
    18181 actcgttgtt tacgttttgc ctgatgcact acgtttatcc ggcctacatg atctctgcaa
    18241 tatattgaat ttgcgtgatt atgtaggccg gataaggcgt tcacgccgca tccggcatga
    18301 acaaagcgca ttttgtcagc aatctaaccc tcttctttta aagagggtat tgatcactct
    18361 gccacttttt tcttcgccgc acgcttcgct ttggtcgctg cggctatttt ctgcgttttc
    18421 gccgatggct ttttcgcttt tctcgcgtct ttcttcgccg ctttcggctt cgtttttttc
    18481 tcaccgcgga aggcgctgtc tggctcaaag tttacctttt taccgacctg acgacgcttg
    18541 ccgccttttt tgcctgcatc gccttttttc gctttctcgc gcgccgtttt accgacgttg
    18601 cgcggtgcgc gttcactgga gatcagacta aagtcgattt tgcgctcgtc catattaacc
    18661 gcttcgactc gaacttccac gcgatcgccc aggcgataag tctggccgct ggattccccc
    18721 atcaggcgtt gccctacctg gtcaaagcga tagtagtcat tgtccagcga agagacatgg
    18781 accagaccat caatgaacaa gtcgtccaga cggacgaaga agccaaagcc agtgacgctg
    18841 gaaattacgc ctttaaagac gttacctacc tggtcgagca tgaagtcaca cttcagccag
    18901 tcagcgacat cgcgcgttgc ttcgtcggca cgacgttccg ccatcgaaca gtgctgaccc
    18961 agttgcaaca tctcttccat cgaataatgg tagccgccgg tttcagtggt gttgccctga
    19021 tgcccctgct ctttcgccag cagatattta atggcgcggt gcagcgtcag gtcaggataa
    19081 cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg ccagaccgaa gtgaccacgg
    19141 ttttctggat cgtaaatcgc ctgtttcatc gagcgtagca gcatggtttg cagcatttct
    19201 gcatcaggac ggtcggcaac cgactccagc agttccgcgt agtcacgcgg ttccggcttg
    19261 ttaccacccg gcagctccag ccccagctcc gccagcactg aacggaaaga ggtaatcgct
    19321 tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt ctttcgcttt ctcaacgaaa
    19381 cgcgccgccg agatattcgc cagaatcatg cactcttcaa ttaatttgtg cgcgtcgtta
    19441 cgctgggtct gttcgatacg ttcaatacgg cgttcagcgt tgaaaatgaa cttcgcttct
    19501 tcgctctcaa atgagatccc accgcgttct tcacgggctt tatccagcac tttatagagg
    19561 ttatgcaact cttcgagatg cttaaccagc ggggcgtact gctcgcgcag atcctgatcg
    19621 ccctgcagaa tatgccagac tttggtgtag gtcagacgcg cgtgagagct catcaccgct
    19681 tcgtagaatt tgtagcccgt caggcggcct ttcgacgaaa ccgtcatctc gcacaccata
    19741 cacaggcggt ctacctgcgg gttgagcgaa cacaggccgt tagagagcac ttccggcagc
    19801 atcgggataa cctgcgaagg gaagtacacc gacgtgccac ggttacgcgc ttctctgtcc
    19861 agcggcgttg gcggacgcac atagtagctg acgtcggcaa tcgcgaccca taaacgccag
    19921 ccgccgctgc gttttttctc gcagtaaact gcatcgtcaa agtcacgggc gtcttcgcca
    19981 tcaatggtga ccagcggtaa atcgcgcaga tcaacacggc ccgcttttgc ttcttccggc
    20041 acttcttctt tcagcccggc aacctgttgc tcaacagcct gcggccagat gtacggaatt
    20101 tcatgggtac gcagagcgat atcaaccgcc atgccggtgc ccatattgtc gcccagcact
    20161 tcgacgattt tacccaccgc tttggtgcgg cgagtcggac gctgagtcag ttcgactacg
    20221 accacaaagc ccatccgcgc gcccatgatc tgatcgggcg ggattaagat atcgaagctc
    20281 agacggctat cgtcaggaac cacaaagccg acgcccgctt cagtaaagta gcgaccaaca
    20341 atctggctgg tttttggcac cagtacgcgg acaatacgcg cttcacgacg acctttacgg
    20401 tcagcgccca gcggctgcgc cagcacctga tcgccatgaa tgcaggtttt catctgctcg
    20461 ctggagagat acaaatcatc tttacgccct tcaacccgca gaaagccgta gccatcacgg
    20521 tggccaataa cggtaccttt caccaggtcg aggcgttccg gcagcgcata gcactgacga
    20581 cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca ggcggcgacg caggccttca
    20641 agctgctctt cgccttcaat gtgcagttct accgccagct catcacggct ggccggtttt
    20701 tcacgtttgg ttaaatgttc gaggataaat tcccgactag ggatgggatt cgcgtatttt
    20761 tcagcttcgc gttcctggaa aggatcttgt gacatctcgg ttcctccgtt gtcatctctg
    20821 atgaagattt tcgtcactcc accagcaata atttataaag cggttgattc tcttcaacca
    20881 aatcggcaag cgtgtagtta tccagttccg taagaaaact ttgcacggcc ttagaaagcg
    20941 cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc actgctgcaa ttcaccagcg
    21001 ataagggctc cagctcgcgc accacatcac caatacgtat cgcactcgcc ggtttaccca
    21061 ggcgaatgcc gccatttttt ccacgaacag cagtcacgta gccggcacga ctaagttgat
    21121 tgattatttt gaccatatga ttacgggaga cgccgtagac gtcagtcact tcagaaatac
    21181 tggtcatccg cccttctggc aatgacgcca tgtagatcag cgcacgtaat ccgtaatcag
    21241 tgaaactcgt taactgcaca tcaacctcaa aagggaaatc gggaaaaaat acatttatat
    21301 tgatgataaa ccagccacaa gctgtgtcgc taatttattt cagttcgggg aaggaaaaaa
    21361 gcgaggattt aacactgtgt cggatagcgt aatggcttaa ccgacacagc aatgtatcac
    21421 actgttagcc cggcaggcaa aatatctgcc aggcgtacca gaattacgcg tcgaacggat
    21481 cgcgcagaat catggtttca gtacgatccg gaccggtaga gatgatatcg atcggcacac
    21541 cagtcagctc ttcaatacgc ttgatgtagt tcagcgccgc ctgcggcaga ccgctacgat
    21601 ctttcacgcc gaaggtggat tcagaccagc ccggcatggt ttcgtaaatc ggctctacac
    21661 ctttccagtc gtcagctgcc agcggagtgg tcgtcacttc acgaccatcc ggcatacggt
    21721 aagccacaca gagtttcacc tctttcaggc catccagaac gtccagttta gtcaggcaga
    21781 agccagacag ggagttcagc tgtaccgcac gacgaacggc aacggtgtcc agccagccgg
    21841 tacgacgacg acgccccgta gttgcgccga attcgttacc ctgcttgcag aggaactcgc
    21901 cagtttcatc aaacagttcg gtcgggaacg gacctgcacc tacacgagtt gagtaagctt
    21961 tgaggatacc cagaacataa tcaacataac gcgggcccag gccggaaccg gtcgccacgc
    22021 caccagcagt ggtgttggaa gaagttacgt acggataagt accgtggtcg atatccagca
    22081 gcgtaccctg cgcaccttca aacatgacga aatcgccacg ctgacgcgcc tggtcgagca
    22141 ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc aacagccatc gtatcatcca
    22201 gaactttctg gtaatcaacc gcttcagctt tgtagtagtt aaccaactgg aagttgtgat
    22261 attccatcac ttctttcagt ttttcagcga aggtttcttt gtcgaaaagg tcgccaacac
    22321 gcagaccgcg acgtgctact ttatcttcat aagcaggccc gataccacga ccggtggtgc
    22381 cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc cagcgcaacg tgataatcaa
    22441 ggatcagcgg acatgcttca gacagcagca gacgctcacg aacggggatg ccacggtctt
    22501 ccagttcttt catctctttc atcagcgcgg ccggagacag cacaacaccg ttaccgatga
    22561 tgctggttac attctcgcgg agaatacctg atggaataag atggagaacg gttttttcac
    22621 cgttgattac gagagtatgg cctgcgttgt gaccgccctg gtagcgtaca acatatttag
    22681 cccgttcagt cagaagatcg acgatcttac ctttaccttc gtcaccccat tgggtgccca
    22741 gtacgacgac gttgttaccc atttttcaaa atcaccgttt gcttaaaaat ggattctacc
    22801 atcgcttttt cagagttaca gcactttttg cactcaaaaa tgacctcgat cagtctgttt
    22861 tttgttcagc caatcgtttt cctcaacatg tagtagacca caacgcccgc aaccacaagt
    22921 ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg tcatcgcaga gatcatcttc
    22981 ttccatgcct tcgggtaaag catcggccct aaaccttcca gtaccaaaac cagggcaagc
    23041 gccagccaga ttgtcgaatt cattcattat ccttatagaa aaagaaaacc accgacatcc
    23101 ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt tatattaacg cgttgcggaa
    23161 gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc tcatgaccat cacgtcctga
    23221 ttgccagaga agctgttctc ataagcacgc aggctacgga tgaatgcgta gaagtccgga
    23281 tctttactga atgcatcagc aaacagtttg gctgcttcgg catcgccttc accacgcatg
    23341 atgcggccct gacgctcagc ttctgccagc gttctggtca cttcatagtc ggcagtcgcg
    23401 cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac gacgcgctac cgcttcacgc
    23461 tcggcgcgca tacggttgta gatcgcttca gacacttcgg tcggcaggtt gatctgcttg
    23521 atacgcacat cgacaacttc aatacccagc gccgccatac tgttcgggtt gatgaccgga
    23581 actttgccct tcgtctcagc cgttacgcgc tctgccgctt cggcaatggc gttatctgct
    23641 gccggggtag taacttcatc ttctgtaccc gcagaaccgg agttcagcgc gtcacgtact
    23701 tcgagggtca gacgaccacg ggaatcagtg acgatatctt tcacgtccag gcgaccaatt
    23761 tcagaacgca gacggtcaga gaacttacgt ttcaacagca cttccgcttg cgaaatgtcg
    23821 ccaccacccg ttgccaggta gtaacggctg aaatcgctga tgcgccattt gatgtaagaa
    23881 tcgacgatca ggtctttctt ctctttggtc acaaagcggt cggcctggtt gtccatggtc
    23941 tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg gtatcttgaa atgcagaccc
    24001 ggctcataaa ccagaggttt gttgtcatcg tcacgcagta ccttaccaaa acgcagcgta
    24061 ataccgcgct caccttcttt gacgacaaag acagacatgt aaagcactac cagcacgatg
    24121 atgataatcg cgataactga cttacgcatc gttattcccc ctgacgctgg tagtcgttac
    24181 gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg actggtggac gacgtgttgc
    24241 ttgctccact ggttgtggag gaagaggctg gcggcagacg cagcagattg ctggcaccgt
    24301 tatcgctctt cgccgcaggg gcgttaccac ctttcagcat ctggtctaac ggcagaacca
    24361 tcaggtttcc acctttgtcg ttaaccagca ctttgcgggt gttgcccaac actttttcca
    24421 tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc ggctttatat tccggcagaa
    24481 gtttagcaaa gcgcgccact tcaccctgag cttccaggat ggtctgggcc ttgtacgcac
    24541 gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg cggctgaact tcgttggtat
    24601 acgcttctgc ttcacgaatg tattgctgtt cgttttcacg cgcggcaatc gcatcgtcaa
    24661 acgccgcttt tacttcttcc ggcggacgag cagcctggaa gttgacgtcc agcagcgtga
    24721 tacccatgtc atacggacga atcgtctctt ccagttcgcg ctgagtatcg ctacgaatca
    24781 cggtacgacc ttccgtcaga atgcggtcca tggtgtattt accgataact ccacgcaggg
    24841 cgctgtcggt agcctgacgc aggctgtcat ccgggctggt cacgctatac agatattttt
    24901 ccggattggt gacgcggtac tgcacgttca tctcaacgcg cactacgttc tcgtccgacg
    24961 tcagcatcac accagaagcg gccagttcac gcacggcttc cacgttcacc ggtttgactt
    25021 cgtcgataaa cgtcggtttc cagttcagac ccggctcaac cagatggctg aatttaccaa
    25081 agcgtgttac cacgccgcgt tcggcttctt taatggtata gaaaccactg gccgcccaga
    25141 taatgacaat cgctgccgct gcgatggtaa cgacacgacc gccaagctgc gggcgcgggc
    25201 cttgcgatga actgccaccg ccagatccgg tgcctttacc gccgcccaga ccaccgagct
    25261 ttttgctcag tttgcggaag atatcatcta aatcaggtgg cccttgatcg cgaccgcctt
    25321 tgtttccatt tccctcagag ttgccgccag gtttgctgct tccccacggg tcgcggtctt
    25381 gtccgttatt accgggctga ttccacgcca tgtttgtgct ccatatttgt tatgcggtga
    25441 tccctgttgt ctttcgacgc cagaggatat gactccacgc ttcagacgct acgccgttag
    25501 atcaggtaat cgatcaacgc cggttcttgt ttacagaggc gacgccagtc aacgatcggc
    25561 atacgaactt gcagacttac gctgccgtcc tcctccatcc actctttttc tattgcctga
    25621 agctgataaa aacgacttct cagacgccct tcctgcggtg gcagacgcaa tgtatgctgc
    25681 gccacctcgc cggaaagccg ccccgtcaaa gcctgaaaaa gctgtggtat ccccgctccg
    25741 gtctgtgcgg aaagccagac acggatcggt ttgttctctt cgtcccgatc aatacgcggt
    25801 tcgaaatctt ccagcatatc gatcttgttc atcaccagca gggttgggat ctcgtgagcg
    25861 tcgatctctt caagaaccgt attcaccgct tcgatgtttt cttgtacacg cacatccgcc
    25921 gcatcaatga cgtgcagcag taatgtggct tgccgcgtct cttgtaacgt ggctttaaat
    25981 gccgccacca gatcgtgcgg caggtggcga ataaacccta cggtatctgc aagtacggtt
    26041 tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt cgagggtggc aaacaactgg
    26101 tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa gggtagattt accggcgttg
    26161 gtatatccca ccagcgaaac agtaggaacg tcggctttga tacgcgattg ccgcccctgc
    26221 tcacgctgct tttcaactct ttccaggcgt gactgtatct gcacgatgcg attacgcaac
    26281 aaacgacggt cggtttcgag ctgggtttca cccggaccac gcaaacctat cccgcctttc
    26341 tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag ccagatggcg cagctgcgcc
    26401 agctcaacct gcaacttacc ctcatgggta cgcgcacgtt gggcgaaaat atctaaaata
    26461 aggccggtgc ggtcgataac acgacactcg cacaaacgct ccaggttacg ctcttgcgcc
    26521 gggctcaggg catggtcaaa aagaacgacc gaagcacccg tcgctttgac agcttccgca
    26581 atttcaactg ctttaccttc acctacaaaa tactttgggt gcggcgcttt acggctaccg
    26641 gtaatcacct gcaatgcttc gacaccggcg gaagagacca gagattcaaa ctcctggagg
    26701 tcttccatat ctttgtcttg cgtaaaatag atgtgtacca gtaccgcctg ctcaccagca
    26761 tcataacggt caaacaagcg tataaccctc taaatagatc agcggggaac gcaggatcgc
    26821 tggctccccg tgtaaaaaaa acagcccgaa accttattcg gtttcttcgc tgtcctgttg
    26881 cgcggaagta ttctgcgcgc tgctaccatg atggtagtta ctgctggtac cgccaccggc
    26941 gttgttactg tgatgagaaa ccgggcgaga cgggacaaca gtagaaatcg cgtgcttgta
    27001 aaccatctgg ctgaccgtgt ttttcaacag gatcacgaat tgatcaaaag actcgatttg
    27061 cccttgcagc ttaataccat tcaccaaata aatagaaact ggaacacgtt cccgacgcag
    27121 tgcgttcagg aacggatctt gtaaagattg ccccttagcc attctctctt ttccttatat
    27181 gcttatttgt actttgaacc tttcgattct gaaaaaattg cgcacgatac gtctcaattg
    27241 tacacattca gcctgcgata gcaccaacaa cctgtaatac ttcgtcgcgc gcctgttctg
    27301 gtttttcact gtcaagccag tgaacccctt cccaaccacg cagccaggtt atctgccgct
    27361 tcgccaactg tctcgtggcg caaacacctc gataaaccat ttcatcgtat gagatttcgc
    27421 cttcaaggta agaccacatc tggcgataac ccacgcaacg aatggaaggc aagtccgtat
    27481 gcaaatctcc tcgggcaaaa agcgcccgga cttctgcttc aaaacctgaa gccaacatct
    27541 gatgaaaacg ctgctcaatg cgttgatgga gcagttcacg gctcgccggg gcgatggcga
    27601 actgatgcac ctgatacggt agagcgtctc ctgacgtttg cgtcagttcc gttaaagttt
    27661 tacccgaaat gaaaaaaact tccagtgccc gggaaagcct ttgtggatca tttggatgaa
    27721 tccttgctgc cgcaaccgga tctacctcct gaagttgacg atgcaatgac tcccaacctt
    27781 gctctaccgc ctgttgctca attctggccc gcacttccgg gtctgccgac ggtagcggcg
    27841 acaacccttc cagcaatgcc ttgaaataca acatcgtacc gcccactaac agtgggatcc
    27901 gccccgccgc ggtgatatcg gccatttccg ccagcgcatc gcggcgaaaa tcagcagccg
    27961 agtaagcctg cgacggatcg cgaatatcca gcaatcggtg cggcgcggcg agtaactctt
    28021 cagcgttcgg cttcgccgtc ccgatatcca tccctttgta aataagggca gaatcaacgc
    28081 ttatcaactc tactggtaaa attttacgca gctcaatggc taacgccgtt ttaccggagg
    28141 ccgtcggccc catcaaaaaa atcgccttag gcaggctcgc cttactgata tcactcatct
    28201 ttcagggctt ttatcgccgg atgtaaatca acagattgta acagaccacc cggcggcgtt
    28261 ttcacaagtt gcggacataa ccgttccacg tccgccagca gggttatggc ctgtgccatt
    28321 gaccactgcg catgttcgct catcagattt cgtgcaatcc actgcgcaat attgccaggt
    28381 tcgaatacgg actgcttcgc caggtagcct atcagttcag gaatcaagat ttgtaaattt
    28441 tgttggcgta agggtaaagg cacggccctg atggtcacat gctgtgcatc tgactggaaa
    28501 tcaataccca attccgccag ggcagactgc gctttttcta atgccgattt ttcttcgcca
    28561 gaaactttta gccgcaacgg aatcagcaac ggctgggcgc aaactggcgc ttcacccggc
    28621 gtcagttgcg cctgacgcag ccaacgttct gccactggca aggctaaaag tgaaatgttg
    28681 ccatcgcgct ccagcaacgc acagtcggaa tggacgatag tcagtacccg accaaaactc
    28741 tgactgttcg ccgcaagtgc aggttcctgc ggttccggcg cttttggttt ttgcatcggc
    28801 gcgggcgttt gcaaaagctg gcgatacact tcaccttgct gtttctggta gcctggctgc
    28861 gcattcggcc agggggcagc cggacgactg cctgaggcgg gcgcaggaga gtagcgcgga
    28921 gctaccggct cacgagctgc cggttctgca aagtgattgc gccccgccgc cacgcggttt
    28981 tccggaatgg gacgcggtgc aggttggggt tcatcgtcca gcggtagcgg cgtttccagt
    29041 tgctgttgta gcacgctcag cacgccctga tagataaaat catgcaccag acgcgactga
    29101 tggaaacgca cttcgtgttt ggcggggtgt acgttgacgt ccacctgatg cgggtcgatc
    29161 tccagataca acacaaacgc cggttgttga tcggccccca gtttgtcttc gcaggcctgg
    29221 cggatcgcat gattgatcag gcgatcgcgc atcatgcgac cattcacgta gcaatactga
    29281 atttccgcca gtgcgggcgt agtgtgattt ggatcggcca cccagccgcg cagcgtgaga
    29341 tcgccgtgtt gccattcaat cgccagcgct tgttcgagaa aagcggtgcc acaaatcgcg
    29401 cctaagcgcc gttctttttg cccgccttcc ggcactgcgc ggtactgacg cacaatttta
    29461 ccgttatgcg acaggttgat cgtgacgtcg aaacgcgcca gcgcaatgcg gcggatgatt
    29521 tcatcaatat ggctaaattc ggttttctcg gtgcgcagga atttgcgccg cgccggggtg
    29581 ttgtagaaca gatccagcac ctccagcgtc gtccccacag gatgcgccgc cggttttacc
    29641 gtcacgttca tatcgcgccc ttcggcatag gcctgccagg cttcctgctg ttctgcggtg
    29701 cgtgaagtga gcgtcaggcg ggaaaccgaa ctgatactcg ccagcgcctc accgcgaaag
    29761 cccaggctga taatggcttc gagatcgtcc agagaggcga ttttactggt ggcatgacga
    29821 gccagcgcca gcgccagctc atcttttttg ataccgcagc cgttatcacg aatgcggata
    29881 agtttcgccc caccgcgttc gatatcaata tcgatacgcg tcgcacctgc atcgaggctg
    29941 ttttccacca gttctttgac taccgacgca ggtcgctcga ccacctcacc tgcggcaatc
    30001 tggttcgcca gttgtggcgg taagacctga attggcatca atcgtcctta gtttggcagc
    30061 gtgcgatctg gcgtcgtcac cgtactggca gtttgtgccg ttgccccctg cggcgcagat
    30121 tgcatcggat gcgcaaggaa ataattgcgc aggcctttgt aaatggcttc tgccagctgt
    30181 tgttggtaat cgtcgctcgc cagcaaacgt tcttcgctgt tgttgctgat aaaaccggtt
    30241 tcgaccagta ctgacgggat atccggcgaa cgcagaacgc cgagactcgc gtgttctggt
    30301 cggcgtttat gtatttcgcc aatgcgttgc aactgactga tcatactggt cgctacatca
    30361 taccctaccc gttgggaatg accgaactgt aaatccagca ccgcctggct taaatagggg
    30421 tcagactgac tgttcgccag cacatcaccc gccccaccca gcagctccga ctgtttctcg
    30481 tgctgctcca gccagctggc catttcgctg ttggcgcgac ggttagagag cacccatacg
    30541 gaagcgccag tcgcactgcg gttcggtgcg gcatcagcgt gaatcgacac gaggaaattg
    30601 gcgttttgct tacgtgccac atcactgcgc cccatcaccg agataaagta atccccgtca
    30661 cgggttaaaa cgcccttaaa catcggatcg tcattgagca aagtacgcaa tttacgcgcg
    30721 atggcgatgg tgacattttt ctcccgcgta ccaccggggc cgatagcgcc agggtcctga
    30781 ccgccgtgtc cggcatcaat agcgatgata attttatcgc cagtgttagc cgtcgcgcgc
    30841 gctgccggac gcgttaccgt attactgctg ataacacccg tagtgcggtt actttccgtt
    30901 ttaaacggat tgcgcgccgg ttcgctgacg cgcggtgcga caaccgcagg cgtttcaacg
    30961 cgtttcgcaa ccacgggcgg cggaggagga ggtggcggcg catcggcgtt aatcgtaaag
    31021 acgacagtgt aattgctgcc attctgccgc ttcaccgctt cggttttacc gttttcggta
    31081 agatcgacca ccagccgtag cgtttgtgca tctttaggcg ttccagagcg aatcgccttc
    31141 accagattat tgccgctgaa caacaacggc agtccctgaa tcacgcccgt ttgtttgata
    31201 tcgagcgcca cggtgcgttt gctttgatgg ctaaacgcat aatcaggatc gccaataaaa
    31261 ctcaacgtta tccgcgcctg ttgattaccg ttagaaacct gaatatcaga gagcgtcgcg
    31321 gcacccaccg gcgtgcacag cagcagcagc gtcgctacca accaatttct gatgcgatac
    31381 atcatcccgc cacctttcaa aggttaaccg gctaaacgcg ccagcaacaa ttcacccgca
    31441 gaggaaaccg cactcacgcg cgcctcacgg ccttgtgcct gataatcaat gtgtatttcg
    31501 acatccgggt caggaagaac acctgtacct tgttgtggcc actccaccag gcagatggca
    31561 tcgttggcaa aataatcgcg gatccccata aactccagct cctcgggatc ggcaaggcgg
    31621 tacaaatcaa agtgataaac cattaaattg tcgagcgtat agggttcgac cagcgtataa
    31681 gtggggcttt tgacattacc ctgatgaccc agagcttgta aaaagccccg gctaaaggtg
    31741 gttttacctg cgcctaaatc gccatacaga tagattacgg ttgcgccatc gcaggctttc
    31801 gctacccgct cgcccaggtc taatgttgcc tgctcatcag ggagcggaat tactcgattc
    31861 atcatggttt ttatcagtca cttccgggtt aacaatacgc tgtagcgtgg aaaagagatc
    31921 ggttgccagc atcccgcgcg ttccaaaacg cgccgccagt acgtcagctg cagcaccgtg
    31981 cgcgacacag cccgcacagg cggcatcata cggcgacagt ttttgcccaa gcaatgcgcc
    32041 aataatacca gagagcacat cgcccatgcc gccgctcgcc atgcctgcat ttccggcatc
    32101 aataatgcct aaagcgtcag gatgggcggc gaccacggtt ccggcacctt tcagcaccgc
    32161 tacgccgcca taacgttgta ccagacgttt ggcgcaatgt aagcggtcac tttcaatttc
    32221 agcgacggaa cagcctaaca accgtgcggc ctcgccagga tgcggcgtga tcacgcgatt
    32281 gtgacgctta tcgggattga ttgccagcag gttcaatgca tcggcatccc acaacatcgg
    32341 tttgcgaaaa ttctcaactt tttgcagtgc ttttttcccc cactcttgct ggcccagacc
    32401 gggaccaatc accaccacat cggcccattc caggctttcg gtaagagagt ccatcgtcag
    32461 ttcatgcacc atcaattccg gtcgtgcagt cagcagcggc gcaatgtttt cactgcgggt
    32521 cagtactcgg actaaaccag caccagcacg tagcgccgct tcccccgtca tacgaatagc
    32581 ccccgccgtg ccgtgatcgc caccgataat taccagccgc ccgtgatcgc ctttatgcga
    32641 agtcgggcga cgcggtttta gccagtgaga aagttgttct gccgaaaacc gctgaatttt
    32701 cgtctcctga cctgccagcc aactatccag ccccagtgag tcaaaatgca gttgtccggt
    32761 aacatcccgc gcttttccag tgagcaagcc tggtttcagc gcaataaaag tgatggtgtg
    32821 atcggcgttg atcactgcgc ctggcgtagc gccggtttca gccagcaggc cggaagggat
    32881 atcaaccgcc gcaatcggcg caggatggga attagcgtgg tcgattaact ggctaatgga
    32941 ttcgcggggc gcttgctgca agccggtacc gagcagcgca tcaacaatca gatctaccga
    33001 ttcgggccag acaatattcg aagcatggat ctcgcctccc gcgtttaacc atgcttcgcg
    33061 tgccagcgcg gcctcttccg gcaacggttt gtcgctctcc tgggccaaca acgtgacctc
    33121 aatgccgacc gctgtggcca gtcgcgcgac cacgtagcca tcgccgccgt tattaccatg
    33181 accgcacagc accagccagt ggcgggcgtc aggatacgcc gaacgacaca cctggaatgc
    33241 ggcttcgcca gcgcgaagca tcagctcata gagtgtaagc cccagcgcat ctgccgcctc
    33301 acgttctccg cggcggatat cgtcggcgtg ccagacggtg tgtggtatac ttacggggtt
    33361 tttcttcatt gtatggtccg tcatgtcaga gcccctcgat ctcaatcagt tagcgcaaaa
    33421 aattaaacag tgggggctgg aactgggctt tcagcaggta ggtattaccg ataccgatct
    33481 cagcgagtcc gagcccaaac tgcaagcatg gctggacaaa caataccacg gcgaaatgga
    33541 ctggatggca cgtcacggta tgctgcgcgc tcgcccccat gagttattac ccggtacgct
    33601 gcgcgtgatc agcgtgcgga tgaattacct tcctgctaac gccgcatttg ccagcacgct
    33661 gaaaaacccc aaactcggct atgttagccg ttatgcgctg ggccgtgact atcacaaact
    33721 tctgcgcaac cgactcaaaa agctgggcga gatgattcag caacattgtg tttcgctgaa
    33781 ttttagaccg tttgtcgatt ctgcgcctat tctcgagcgc ccgttagctg caaaagctgg
    33841 gctcggctgg acaggtaagc actcacttat cctcaatcgc gaggccggtt ctttcttctt
    33901 tttaggcgaa ttgctggtcg atattccgct gcccgtggat caaccagtcg aggaaggatg
    33961 cggtaaatgc atagcctgta tgacgatttg cccgaccggt gccatcgtcg agccatatac
    34021 cgtcgatgct cgccgctgta tctcttatct caccatcgaa ctggaagggg cgatcccgga
    34081 agagttgcga ccgttaatgg gaaaccgtat ttacggttgc gatgactgcc agcttatctg
    34141 cccgtggaat cgctattcgc aactcactac agaagacgat ttcagcccgc gtaagccgct
    34201 acacgcaccg gaactcattg agttattcgc ctggagcgaa gagaagtttt taaaagtcac
    34261 ggaaggatcg gcgattcgcc gtataggtca cctgcgctgg ctgcgtaata tcgccgtggc
    34321 attaggcaat gccccctggg atgaaacgat tctggcggcg ctggagagtc gcaaaggtga
    34381 gcacccactt cttgatgagc acatagcgtg ggcgatggcg cagcaaatcg agaggcgaaa
    34441 tgcgtgcata gtcgaagtgc aattgccgaa aaaacagcgt ctggttcggg tgattgaaaa
    34501 agggttacca cgtgacgcct gattcatcca cagcttgtga ataaaaataa aaacgcattg
    34561 caattcaaga cgaataaaat cgtcaagtga tcgactcgac aaaacgaaat cttaattttt
    34621 aacttatcta tcaataagtt aaaaaaatac tattcaccgt gcgaagtttc tttgaaatta
    34681 agactacagc ctgaggctgt ggataactct gtttacaaga gtttgtcaga gacaacaaaa
    34741 accagcaata acgcatcgcg tcgctgtgga tattttattg agagaagaat ttggagcggg
    34801 aaacgagact cgaactcgcg accccgacct tggcaaggtc gtgctctacc aactgagcta
    34861 ttcccgcttg ggtggtctgt gccttacagc actttcaaat tttggagcgg gaaacgagac
    34921 tcgaactcgc gaccccgacc ttggcaaggt cgtgctctac caactgagct attcccgctt
    34981 aatcttcgtc tttcgaaccg ctactgcctt ggctgccttc actcacctta gtcacatact
    35041 tttgtaagtt cccaaggatt cgttcagttg ccgccttgtc gcaattcgaa atactgcgat
    35101 tgggtggtct gtgctttgca gcacttttca aattttggag cgggaaacga gactcgaact
    35161 cgcgaccccg accttggcaa ggtcgtgctc taccaactga gctattcccg cgtactactt
    35221 aattttgctt tcgtaatttt cgcatttctg cgtcgttacg ggaggcgcat tatacgagaa
    35281 atctttccct ctgcaacccc ccaaaaaagt tttttttgcg tttttatctc aagtgctgaa
    35341 attatcagca agacgttcaa tttagcgtca aaatcacgcc gccgcaagtg gattccggcg
    35401 cttacgtgac caggaaaaat tttacagctt gataaaatgc tcgcggtagt aagccagctc
    35461 cgccaccgat tcacggatat catccatcgc ctgatgcgtc ccctgcttgg taaaaccatc
    35521 cagaatttcc ggcttccagc ggcgcgccag ctctttcagg gtgctgacat cgagataacg
    35581 gtagtggaag taggcttcca gctccggcat gtatttaaac aggaaacgac ggtcctgacc
    35641 gatgctgtta ccgcaaatcg gcgattttcc cgcaggcacc cactgtttta aaaattcgag
    35701 cgttgccagt tcagcttccc gatcgcccat cgtgctcgct ttcacgcgct ctaccagccc
    35761 gctggcggta tgggtgcgca cgttccagtc atccatcagc gccagctgtt catcagactg
    35821 gtgtactgca atggtcggcc cttctgccag aatattcagg ttggcatcgg tcaccagcgt
    35881 ggcaatctca ataatgcgat cgcgctcggg atccagaccg gtcatctcaa gatcgatcca
    35941 aatcaggttg ttttcattgg cactcatgct attttccacc cattcacagt gactatattg
    36001 atccacaatt gcgtgtatca tagatgtttt gcccatcagg ggcgaccagg agtctgtacg
    36061 attgagtaaa aataaactct ccaaaggcca gcagcgccgc gtgaacgcca atcaccagcg
    36121 tcgtcttaaa acgtctaagg agaagcccga ctacgacgac aatctgtttg gcgagcctga
    36181 tgaaggtatc gtcatcagcc gctttggtat gcacgctgat gtggaatccg ccgatggcga
    36241 cgttcaccgc tgcaatattc gccgtaccat ccgttcgctg gtaaccggcg accgcgtagt
    36301 ctggcgtccg ggtaaaccgg cggcggaagg cgtaaatgtc aaagggatcg tggaagcggt
    36361 gcatgagcgt acctcggtgt tgacgcgtcc ggatttctac gacggcgtaa aacctattgc
    36421 cgccaacatc gaccagattg tcattgtctc cgccattttg ccggagctgt cgctcaatat
    36481 tatcgaccgt tacctggtgg cctgcgaaac cttgcagatt gagccgatta ttgtgctcaa
    36541 caagatagac ctgctggacg acgaaggcat ggcgttcgtc aacgagcaga tggatatcta
    36601 ccgcaatatc ggttatcgcg tattgatggt ttccagccat actcaggatg ggctaaaacc
    36661 gctggaagag gcgttgaccg ggcgcatcag catttttgcc gggcagtctg gcgtcggcaa
    36721 atccagcctg ctgaatgcgt tgctggggct gcaaaaagag atcctgacca acgatgtctc
    36781 tgacaactcg gggctcggtc agcacaccac gaccgccgct cggctgtatc acttcccgca
    36841 cggcggtgat gtgattgatt cccctggtgt gcgtgagttc ggcctctggc acctggagcc
    36901 ggaacaaatc actcagggct ttgtcgaatt ccatgactat ttaggtctgt gtaaatatcg
    36961 cgattgcaaa cacgataccg atccgggctg cgctatccgg gaagcggttg aggaagggaa
    37021 aatcgcggaa acccgtttcg aaaactatca ccgtattctg gaaagcatgg cgcaggtaaa
    37081 aacgcgtaaa aacttttctg atacggatga ctgacaatta agctaaccgt cgttagaatc
    37141 gtcccctttt tttcaggatc cggcatgttg gccggatcag gaacgacaaa acaatggcct
    37201 ggaggctacc ttgttaaatt catttaaact ttcgctacag tacattctgc cgaaactatg
    37261 gcttactcgc ctggcgggtt ggggcgcaag caagcgggca ggatggctga caaaactggt
    37321 tatcgatctg ttcgttaaat actacaaggt cgacatgaaa gaggcgcaaa agccggacac
    37381 cgccagctac cgcaccttta acgaattctt tgtccgtccg ctgcgtgacg aagtacgccc
    37441 aatcgatacc gatccgaatg tcctggtcat gcctgccgat ggcgttatca gccagttggg
    37501 taaaatcgaa gaagataaaa tcctgcaagc caaaggccac aactacagcc tcgaagccct
    37561 gctggcaggc aactatctga tggcggacct gttccgcaac ggtacgtttg tgaccactta
    37621 cctctccccg cgtgactacc atcgcgtaca catgccgtgc aacggtattc tgcgtgagat
    37681 gatctacgtg ccgggcgatc tcttctccgt taaccatctc accgctcaga acgtgccgaa
    37741 tctgtttgcc cgtaacgaac gcgtgatttg cctgttcgat accgaatttg gcccgatggc
    37801 gcagattctg gtcggggcga cgattgtagg cagcattgag acggtctggg cgggcaccat
    37861 tacgccgccg cgcgaaggta tcatcaagcg ctggacctgg cctgccgggg aaaacgacgg
    37921 ttctgtggca ctgctgaaag gccaggaaat gggccgcttt aaactcggct ctaccgttat
    37981 caacctgttt gcaccgggta aagtgaatct ggttgagcaa ctggaaagcc tgtctgtcac
    38041 gaaaattggc cagccgctgg cagtatctac cgaaaccttt gttacgccag acgctgaacc
    38101 cgccccactt cctgctgaag agatcgaagc agaacacgac gccagcccat tggttgacga
    38161 taaaaaagac caggtctaat ccatcaaagg aaacgctgac gtgcgcctga ttatcacttt
    38221 tctgatggcc tggtgcctca gttggggggc gtacgccgcg acggcccccg atagcaaaca
    38281 aatcactcag gaactggagc aggcaaaagc ggcgaaaccc gcacagccgg aagtcgtaga
    38341 ggcgctccag tctgccttaa atgcgcttga ggaacgaaaa ggttcccttg agcgcatcaa
    38401 acaatatcag caagttatcg ataattatcc gaaactctcc gctactctgc gcgcacaatt
    38461 aaacaacatg cgtgacgagc cgcgcagcgt gtcgccggga atgtctaccg acgcgctgaa
    38521 tcaggaaatt ctccaggtca gcagccagtt gctggataaa agccgtcagg cccagcaaga
    38581 gcaggagcgc gcccgcgaga ttgccgattc gctgaatcaa ctgccgcaac agcaaaccga
    38641 cgcccgccgt cagttaaatg agatcgagcg ccgcctggga acgcttactg gcaatacccc
    38701 gctcaatcag gcacaaaatt tcgcgttgca gtctgactcc gcacgcctga aagcgctcgt
    38761 tgatgaactg gaactggcgc agctgtctgc caataaccgt caggaattag cgcgcttacg
    38821 ctcagagctg gcggaaaaag agagccagca actggatgcg tatttgcagg ccttacgtaa
    38881 tcagttgaac agccaacgtc agcttgaggc ggagcgggcg ctggaaagta ccgaattgct
    38941 ggcagaaaac agcgccgatt tgccgaaaga catcgtcgcg caattcaaaa ttaaccgcga
    39001 actatcggcg gcactgaatc aacaggcgca gcggatggat ctcgttgcct cgcaacagcg
    39061 ccaggccacc agccagacgt tacaggttcg gcaggcgctg aatacgctgc gtgaacagtc
    39121 gcaatggctg ggatcgtcca acctgctcgg cgaagcgttg cgggcgcagg tggcacggct
    39181 gccggaaatg ccgaaaccac aacagcttga taccgaaatg gcgcagttgc gtgtgcaacg
    39241 gttacgttat gaggatctgc ttaataaaca gccgctgcta cggcaaattc atcaggccga
    39301 cggtcagccg ctgaccgccg agcaaaaccg tattctggaa gcacagctac gcactcagcg
    39361 tgagttgctg aactcattgt tgcagggtgg cgacacgcta ctgctggaac tgaccaagct
    39421 gaaagtctcc aacgggcaac ttgaggatgc gctgaaagag gtgaatgaag cgacgcatcg
    39481 ctatctgttc tggacctctg acgtgcgccc gatgaccatc gcctggccac tggaaatcgc
    39541 ccaggatctg cgtcgtctca tttcgctgga caccttcagt cagttgggca aagccagtgt
    39601 gatgatgctg accagcaaag agacgatttt gccgctgttt ggcgcgttga ttctggtcgg
    39661 ttgcagtatt tactcgcgcc gctatttcac ccgttttctt gaacgttcgg cggcgaaagt
    39721 cggcaaagtg actcaggatc acttctggct gacgttgcgc actcttttct ggtcgattct
    39781 cgtcgcgtca ccgctgccgg tgctgtggat gacgctgggt tacggcttgc gcgaggcgtg
    39841 gccttatccg ctggcggtcg cgattggcga tggcgtaacg gccaccgtgc cgctgctgtg
    39901 ggtagtgatg atttgcgcca cctttgcccg cccgaacggc ttgtttatcg ctcattttgg
    39961 ctggccgcgc gaacgtgttt cccgtgggat gcgctactac ctgatgagca tcgggcttat
    40021 tgtgccgctg attatggcgc tgatgatgtt cgataacctc gacgaccgtg aattctccgg
    40081 ttcgctggga cggctttgct ttatcctcat ttgcggtgcg ctggcggtgg tcaccctcag
    40141 cctgaaaaag gccgggatcc cgctgtacct caacaaagag ggcagcggcg acaacattac
    40201 caaccatatg ctgtggaaca tgatgattgg cgcaccactg gttgccattc tggcgtcagc
    40261 ggtgggttat ctggcaacgg cgcaggcact gttagcgagg cttgaaacct cggttgccat
    40321 ctggttcctg ctactggtgg tttatcacgt tatccgccgc tggatgctga tccagcgtcg
    40381 caggctggcg tttgaccggg cgaagcatcg ccgggcagag atgttagcgc aacgcgcgcg
    40441 tggcgaagag gaagcgcatc atcacagtag cccggaaggg gcaattgaag tcgatgaaag
    40501 cgaagtcgat ctcgatgcca tcagtgcgca atccttgcgg ctggtgcgct caattttgat
    40561 gttgatcgcc ctgctttctg tcattgtgct gtggtcagaa atccattccg ctttcggctt
    40621 cctcgaaaat atttcgctgt gggatgtcac ctccacggta cagggcgtag aaagtctgga
    40681 gccaattacc ctcggtgcgg tgctgattgc cattctggtg tttatcatca ccacgcagct
    40741 ggtgcgcaac ctgcccgcgc tgctggaact ggcgattttg cagcacctgg atttaacgcc
    40801 gggtacgggc tacgccatca ccaccatcac caaatatctg ctgatgctga ttggcgggct
    40861 ggtcggcttc tcgatgattg gtattgagtg gtcgaaattg cagtggctgg ttgccgcgct
    40921 cggtgttggt ctcggttttg gtttgcagga gattttcgcc aactttatct ctggcctgat
    40981 tatcctgttc gaaaaaccga ttcgcattgg cgatacggta acgatccgcg atcttaccgg
    41041 tagcgtgacg aaaattaaca cccgcgccac caccatcagc gactgggacc gtaaagagat
    41101 aatcgtgccg aacaaggcgt ttattaccga gcagtttatc aactggtcgc tctctgactc
    41161 ggtcacgcgc gtggtgttga cgataccggc ccctgccgat gccaatagcg aagaagtgac
    41221 ggaaatcctg ctcaccgcag cgcgtcgctg ctcgctggtg atcgacaacc cggcaccgga
    41281 agtcttcctg gtggatctgc aacaggggat tcagattttc gagctgcgta tttacgccgc
    41341 tgagatgggt caccgtatgc cgctacgcca tgagatccac cagctgattc tggcaggctt
    41401 ccacgcccac ggtatcgata tgccattccc gcccttccag atgcgtctgg aaagcctcaa
    41461 cggtaaacaa acggggagaa cgctgacgtc tgcgggcaaa ggtcgtcagg cgggaagttt
    41521 gtaagtttgt gtgccggatg aaccaatccg gctcacaaac aaaactgata gcgcagaaaa
    41581 aactgccaca gccagtagcc gaaaatagcc agcgcggtgg cgtacataaa ccagccgcgt
    41641 ttttttcgcc acaaagcgaa ccagagaaaa gggaaaaaga gcggtaacgt cattatcaca
    41701 ccaacatcac gggtatcgtc ttcttcgaga tacatcaaca catcgcagac gttgttaatc
    41761 ccgcgtccat caatcatcca ctctttgtcc agcgcggaag tgatggttat ccagaggaac
    41821 gccacaataa accagatgca gcataagaca aacatgcgcg cactcataag gtaaccggtc
    41881 gcttaatttc gctatagcgc atacgaaaca aatcgccgta tcccactaat tccccgcgaa
    41941 aaaccagaca tcggataaga taagcgcaat aaatatccgg caggtttccc caccgtttac
    42001 catcgcgatc tttgagggtg ttaatggtgc tacccaccac catcgccact ttacgggtgt
    42061 ggcaggcatc gacaaaagag agaatcgtct gctctatttc cgctacatcc ccggcagaaa
    42121 attgcagcgc cctgatttca tcttcggtta ttgccgggtc ccggctggtg tcatccataa
    42181 catttcctgt ctttacgccc tatcgtcagg tagacgatag ggcatcgggt tattccaccg
    42241 cccactgcgg attttttgcc actctgctgc aatagttctg gtaaatcgcc atcgctaaca
    42301 gcgagaagaa gacagggccg ccaatcatcc acaatgtgct gtcccagtct ccggcttcaa
    42361 ccacaggttg aatgatggtg aagacgttcg caaatgtcac caccagtacg acaaccactg
    42421 ttgcaatcat tgccgacata tgcgttttga aaatcacaaa tggtctgtcg agatcctgac
    42481 gtgctttaaa aaatgggaaa gccagcgcga ggaacaggta aggaagcgtc atagacacgt
    42541 tcgccatcag cgtcagcttg ttaaagaacg ccgatgcggt accgccacca aacgaaacca
    42601 gcaggatgaa gacagtaacc aacccgcact gcatccacat ggcgatagac ggcatcccca
    42661 tcgcattcag gcgcgtcatc ggttccggcc acaacgcttt cggcgtcccc tggatgatgg
    42721 ctttcagcgg tgaatagcaa agcgtaaaga acgcaccggt ataagcgagg aacatcgaaa
    42781 gtccggtaat acgcgcaaac catacgccca gcgacaatga cgcttcaggt gacaaatgca
    42841 gtgcgttacc cagcgtcacc ccgaggctct tcatcagcac ataggtaata ttgccaaggt
    42901 taacggaacc attacttaat acctgctgcc agtttgtgct gacgccccat aaaaatattg
    42961 ccagcgaata accgattgaa ataacaatag cggcaaaaac aatacctttg gcaaagttct
    43021 tttctggatt ttccgtttta tcgaccagac caccaaccgc ttcaattccg ccataggcaa
    43081 aaatagcaaa taccacaaac gatagcattg ccagaccgga ctgataacct gggttcggtg
    43141 atgcaaggaa attaatatcc tgcgcgaaat gcccaccatt taataacaaa atagtaatgc
    43201 ttactaacag caatactaaa ttcagacaca ttactgcaat accgcccacc gcagtaatgc
    43261 gggcaatttt attaatcccc tttgaagcaa cgacggtgac cagaatcatc catgccactg
    43321 ccagcagacc aaccacctgc gtaggctcca gtccagcaat acgccagtgc tgggtcatgt
    43381 cgctaccata gaggaatgtt gagaacggta cccaaacttt cgcggaggta ctcaccatcc
    43441 agatgatata agaggaaaac cacataaacg taccaatgaa ggcaaaacgt ggtccgacac
    43501 tattattcat ccaggaatag ataccgcctt cttctttgcg ataagcagct cccatttcag
    43561 ccatcattaa ggcgaatgga ataaagaata acaatgcaga aaatatataa aagggaatcg
    43621 cactataacc cattaagtaa taagccgatg ggctattggc aaatccaaat acggaagtaa
    43681 agatcatcaa tatgagtcct atcagactca tcttttttat cgtgtgaggc atgaaaccat
    43741 ccttcatttc agcgcgtgta taacacgtgc ccactgaact gcactgcgca acattgagca
    43801 ggcagcgtaa aaaccaatac agaattgata cctggatgat atcaaatatc cagtcttaaa
    43861 ttgtggcctt taacggaaaa atgaaaacat atgctacaga atggcgctta tcacgccatt
    43921 cttcgctgtt aattcagtaa tttttcagaa ttatgcccgg tcaacgctaa aggcgatgac
    43981 ttcagccagt gtctccgcgc ccagcgccaa catcaccaga cgatcaacac ctaatgccac
    44041 gccggaacag tcaggcatac cgactttcaa ggcttcaatc agattctggt caatggggtg
    44101 ctgcggcaaa ccgcgcgccg cgcgcttacg gttatcttgt tcaaagcgtt gttgctgctc
    44161 acgggcatcc gtcaattcat ggaaaccatt cgccagctca atacctttat aataaacctc
    44221 aaagcgttca gcgacccgat gatcttcggt actgatttgc gccagtgatg cctggctggc
    44281 tggaaagtgg tacacaaagg tcggtttttc tttgccaata tttggctcta cgccaaaggt
    44341 aaacagcaat tgtagcagcg tgtcgcggtc ttcttcggta tcagcaacat tgctcaaatc
    44401 cagtttcgct gcgacttccc gcagttgcgt tttgtcggca gagagcgggt caatttccag
    44461 ataacgcaag aaagcttgtt gataagaaag gctttctgct gccgggcagt ccagcacctg
    44521 ttgtaagaga tcgtccacct cgttcatcaa ccggtacata tcatagtgcg gtcgatacca
    44581 ctccagcata gtgaactcag ggttgtgata acgccccatc tcttcattac ggaagctgcg
    44641 gcacagctgg aataccggcc cacaaccggc aaccagcagg cgtttcatat ggtattccgg
    44701 gctggtcatt aaccagagat tcatcccctg cgaatgcccg gggccaacga aacgtgtctc
    44761 aaacgggacc aaatgaatat cggttaccgt cgcctggctc atacaaggcg tctccacctc
    44821 cagcactcca cgatcggcaa agaaacgacg gatctccgcc ataatcgccg cgcgttttaa
    44881 taagttagga atggatgcgc tcggctgcca ggatgccgtt tcgctcatag ttaaatctcc
    44941 agtttttgac aagggcacga agtctactcg caacgcgacg gcgagacaaa ttttacgcag
    45001 gaatcaaaca gcggtgggca gtgactaaaa aaagcacgat ctgatggttt agtaattaaa
    45061 ttaatcatct tcagtgataa tttagccctc ttgcgcacta aaaaaatcga tctcgtcaaa
    45121 tttcagactt atccatcaga ctatactgtt gtacctataa aggagcagtg gaatagcgtt
    45181 cgcagaccgt aactttcagg cacttaccct gaagtacggg gctgtgggat aaaaacaatc
    45241 tggaggaatg tcgtgcaaac ctttcaagcc gatcttgcca ttgtaggcgc cggtggcgcg
    45301 ggattacgtg ctgcaattgc tgccgcgcag gcaaatccaa atgcaaaaat cgcactaatc
    45361 tcaaaagtat acccgatgcg tagccatacc gttgctgcag aagggggctc cgccgctgtc
    45421 gcgcaggatc atgacagctt cgaatatcac tttcacgata cagtagcggg tggcgactgg
    45481 ttgtgtgagc aggatgtcgt ggattatttc gtccaccact gcccaaccga aatgacccaa
    45541 ctggaactgt gggggtgccc atggagccgt cgcccggatg gtagcgtcaa cgtacgtcgc
    45601 ttcggcggca tgaaaatcga gcgcacctgg ttcgccgccg ataagaccgg cttccatatg
    45661 ctgcacacgc tgttccagac ctctctgcaa ttcccgcaga tccagcgttt tgacgaacat
    45721 ttcgtgctgg atattctggt tgatgatggt catgttcgcg gcgtggtagc aatgaacatg
    45781 atggaaggca cgctggtgca gatccgtgct aacgcggtcg ttatggctac tggcggtgcg
    45841 ggtcgcgttt atcgttacaa caccaacggc ggcatcgtta ccggtgacgg tatgggtatg
    45901 gcgctaagcc acggcgttcc gctgcgtgac atggaattcg ttcagtatca cccaaccggt
    45961 ctgccaggtt ccggtatcct gatgaccgaa ggctgccgcg gtgaaggcgg tattctggtc
    46021 aacaaaaatg gctaccgtta tctgcaagat tacggcatgg gcccggaaac tccgctgggc
    46081 gagccgaaaa acaaatatat ggaactgggt ccacgcgaca aagtttctca ggccttctgg
    46141 cacgaatggc gtaaaggcaa caccatctcc acgccgcgtg gcgatgtggt ttatctcgac
    46201 ctgcgtcacc tcggcgagaa aaaactgcat gaacgtctgc cgttcatctg cgaactggcg
    46261 aaagcgtacg ttggcgtcga tccggttaaa gaaccgattc cggtacgtcc gaccgcacac
    46321 tacaccatgg gcggtatcga aaccgatcag aactgtgaaa cccgcattaa aggtctgttc
    46381 gccgtgggtg aatgttcctc tgttggtctg cacggtgcaa accgtctggg ttctaactcc
    46441 ctggcggaac tggtggtctt cggccgtctg gccggtgaac aagcgacaga gcgtgcagca
    46501 actgccggta atggcaacga agcggcaatt gaagcgcagg cagctggcgt tgaacaacgt
    46561 ctgaaagatc tggttaacca ggatggcggc gaaaactggg cgaagatccg cgacgaaatg
    46621 ggcctggcaa tggaagaagg ttgcggtatc taccgtacgc cggaactgat gcagaaaacc
    46681 atcgacaagc tggcagagct gcaggaacgc ttcaagcgcg tgcgcatcac cgacacctcc
    46741 agcgtgttca acaccgacct gctctacacc attgaactgg gccacggtct gaacgttgct
    46801 gaatgtatgg cgcactccgc aatggcacgt aaagagtccc gcggcgcaca ccagcgtctg
    46861 gacgaaggtt gcaccgagcg tgacgacgtc aacttcctca aacacaccct cgccttccgc
    46921 gatgctgatg gcacgactcg cctggagtac agcgacgtga agattactac gctgccgcca
    46981 gctaaacgcg tttacggtgg cgaagcggat gcagccgata aggcggaagc agccaataag
    47041 aaggagaagg cgaatggctg agatgaaaaa cctgaaaatt gaggtggtgc gctataaccc
    47101 ggaagtcgat accgcaccgc atagcgcatt ctatgaagtg ccttatgacg caactacctc
    47161 attactggat gcgctgggct acatcaaaga caacctggca ccggacctga gttaccgctg
    47221 gtcctgccgt atggcgattt gtggttcctg cggcatgatg gttaacaacg tgccaaaact
    47281 ggcatgtaaa accttcctgc gtgattacac cgacggtatg aaggttgaag cgttagctaa
    47341 cttcccgatt gaacgcgatc tggtggtcga tatgacccac ttcatcgaaa gtctggaagc
    47401 gatcaaaccg tacatcatcg gcaactcccg caccgcggat cagggtacta acatccagac
    47461 cccggcgcag atggcaaagt atcaccagtt ctccggttgc atcaactgtg gtctgtgcta
    47521 cgccgcgtgc ccgcagtttg gcctgaaccc agagttcatc ggtccggctg ccattacgct
    47581 ggcgcatcgt tataacgaag atagccgcga ccacggtaag aaggagcgta tggcgcagtt
    47641 gaacagccag aacggcgtat ggagctgtac tttcgtgggc tattgctccg aagtctgccc
    47701 gaaacacgtc gatccggctg cggccattca gcagggcaaa gtagaaagtt cgaaagactt
    47761 tcttatcgcg accctgaaac cacgctaagg agtgcaacat gacgactaaa cgtaaaccgt
    47821 atgtacggcc aatgacgtcc acctggtgga aaaaattgcc gttttatcgc ttttacatgc
    47881 tgcgcgaagg cacggcggtt ccggctgtgt ggttcagcat tgaactgatt ttcgggctgt
    47941 ttgccctgaa aaatggcccg gaagcctggg cgggattcgt cgacttttta caaaacccgg
    48001 ttatcgtgat cattaacctg atcactctgg cggcagctct gctgcacacc aaaacctggt
    48061 ttgaactggc accgaaagcg gccaatatca ttgtaaaaga cgaaaaaatg ggaccagagc
    48121 caattatcaa aagtctctgg gcggtaactg tggttgccac catcgtaatc ctgtttgttg
    48181 ccctgtactg gtaaggagcc tgagatgatt aatccaaatc caaagcgttc tgacgaaccg
    48241 gtattctggg gcctcttcgg ggccggtggt atgtggagcg caattattgc gccggtgatg
    48301 atcctgctgg tgggtattct gctgccactg gggctgtttc cgggtgatgc gctgagctac
    48361 gagcgcgttc tggcgttcgc gcagagcttc attggtcgcg tattcctgtt cctgatgatc
    48421 gttctgccgc tgtggtgtgg tttacaccgt atgcaccacg cgatgcacga tctgaaaatt
    48481 cacgtgcctg cgggcaaatg ggttttctac ggtctggctg ctatcctgac agttgtcacg
    48541 ctgattggta tcgttacaat ctaacgtatc gccaatgtaa atccggcccg cctatggcgg
    48601 gccgttttgt atggaaacca gaccttatgt tcaaaacgac gctctgcgcc ttattaatta
    48661 ccgcctcttg ctccacattt gctgcccctc aacaaatcaa cgatattgtg catcgcacaa
    48721 ttaccccgct tatagagcaa caaaagatcc ccggtatggc ggtggcggta atttatcagg
    48781 gtaaacctta ttactttacc tggggctatg cggacatcgc caaaaagcag cccgtcacac
    48841 agcaaacgtt gtttgagtta ggttcggtca gcaaaacatt tacgggcgtg cttggtggcg
    48901 acgctattgc tcgaggggaa atcaagttaa gcgatcccac aacaaaatac tggcctgaac
    48961 ttaccgctaa acagtggaat gggatcacac tattacatct cgcaacctac actgctggcg
    49021 gcctgccatt gcaggtgccg gatgaggtga aatcctcaag cgacttgctg cgcttctatc
    49081 aaaactggca gcctgcatgg gcgccaggaa cacaacgtct gtatgccaac tccagtatcg
    49141 gtttgttcgg cgcactggct gtgaagccgt ctggtttgag ttttgagcag gcgatgcaaa
    49201 cacgtgtctt ccagccactc aaactcaacc atacgtggat taatgtaccg cccgcagaag
    49261 aaaagaatta cgcctgggga tatcgcgaag gcaaggcagt tcatgtttcg ccaggggcgt
    49321 tagatgctga aacttatggt gtgaagtcga ccattgaaga tatggcctgc tgggtacgaa
    49381 gcaatatgaa tccccgtgat atcaacgaca aaacacttca gcaagggata caactggcac
    49441 aatctcgcta ctggcaaacc ggcgatatgt atcagggcct gggctgggaa atgctggact
    49501 ggccggtaaa tcctgacagc atcattaacg gcagtggcaa taaaattgca ctggcagcac
    49561 accccgtaaa agcgattacg cccccaactc ctgcagtacg cgcatcatgg gtacataaaa
    49621 caggggcaac cggcggattt ggtagctatg tcgcgtttat tccagaaaaa gagctgggta
    49681 tagtgatgct ggctaacaaa aactatccca atccagcgag agtcgccgcc gcctggcaga
    49741 ttctcaacgc tctacagtaa aatttcatcg ggtccgaatt ttcggacctt ttctgcgctt
    49801 ttccttgctg tcatctacac ttagaaaaaa accagtaagg aaacatttat gcgcctgctc
    49861 cctctcgttg ccgcagcgac agctgcattt ctggtcgttg cctgcagttc tcctacgccg
    49921 ccgcgtggcg taacagtagt gaataatttc gacgccaaac gttatctcgg tacctggtat
    49981 gagattgccc gttttgatca ccgttttgaa cgtggactgg aaaaagtcac cgcaacatac
    50041 agcctgcgtg atgacggcgg cctgaatgtc attaataaag gctataaccc tgacagagga
    50101 atgtggcagc agagtgaagg gaaagcgtac tttaccggcg caccaactcg cgctgcgctg
    50161 aaagtgtcat tctttggtcc tttctatggc ggttataacg ttattgcact cgatcgggaa
    50221 taccgccatg cgctggtttg cggcccggac cgcgactacc tgtggatact ctcccgcacg
    50281 ccaaccattt ctgacgaagt gaaacaggag atgctggcag tcgcgacccg ggaagggttt
    50341 gatgtcagta agtttatttg ggtacagcag cccggtagtt agtgagtgct gagtttcaga
    50401 ccaataatcc ccaatacgat taacgccaga ctcgccaggc gcatcgggtt agcggactca
    50461 ccgagcagca caatgccggt gatggccgcg ccgactgcgc caatacccgt ccacacggca
    50521 taagccgtcc ctactggtaa cgatttcatc gcccaggcaa gtagcgccat actgacaatc
    50581 atcgccgtca cggtaataac actcggcgtc aaacgactaa agccgtgggt atatttcagg
    50641 ccaacggccc ataccacttc cagcagacca gcaataacta agataatcca ggacatatca
    50701 ggctccggag aattggggcc gtccccggtg aaaagaggcg tttgcaggtc gtcctgcaaa
    50761 gctgatggtt gaacggatat tttgccaggt ggcaggggaa tttcaactat taccttagtg
    50821 actatcgcag aaaaaacaaa cggcacgaca tacctgtcgt accgttattg cttattgcta
    50881 ttgctgcgct ttcgttgctg cgccagaaat cgcgttaccg ccatcagaaa tgtcttcacc
    50941 aacgccacgc gtggtgttgc aggcagttaa tactgttgaa agcaccagaa cagaaaagat
    51001 cgctgcaatt gtcttcttca ccataacgtc ttccttttag gtaatgttat ttatgtttgc
    51061 ctatagcaaa ttaataatag acaacatcgc caaaaatgac ggaaatctga tgatttttcg
    51121 gaagagaaga aaaattagct ggcagcgcga gagatggagt tgccgagatg tttgatgtct
    51181 tcgccgaaac cgcgagcggt gttacagccc gtgagcagcg tgctggcaag caaaacaaga
    51241 acgataaggc gtttcatcat ctctgacctg aataagtgat ggtgcagcct gcaggccgca
    51301 ccacaaccgc attacttcac gcgagagacg tattcaccag agcgggtatc cactttgatg
    51361 acttcgccga tttgtacgaa cagcggaacc ttaaccacag cgccagtaga cagggtagcc
    51421 ggtttgccgc cagtacctgc ggtatcacct ttcaggcccg gatcggtatc aacgatttcc
    51481 agttcaacga agttcggcgg agtaacggag atcggctgac cattccacag agttacgata
    51541 cactctgcct gatccagcag ccatttagcg ttgtcgccaa ttgcttttgc atcagcagac
    51601 agctgctcga aagtttcgtt gttcatgaag tgccagaact caccgtcgtt gtacaggtaa
    51661 gtcaggttca tatcgacaac atcagcgcct tcagcggaat cagtagattt gaaggttttt
    51721 tctacgcgag taccggtcag cagacgacgc agtttaacgc gagcaaatgc ctggccttta
    51781 cccggtttta cgaattcact cgcttcaacc gcgtaaggtt cgccgtctaa catgatttta
    51841 agaccagcac gaaaatcgtt gctatagtac gttgccataa ggccctctga aatttgttaa
    51901 ttggtagcta agccacaaaa tggcgcatat tgtaacccta aataccccat ccagagaaga
    51961 ttggttaacg caacttgccg atgttgtgac cgatcctgat gaacttctgc gtcttttgaa
    52021 tatagacgcg gacgaaaaac tgttggccgg acgcagcgcc aaaaagctgt ttgccctgcg
    52081 tgtgccccgc tcatttatcg atcgcatgga gaaaggcaat ccggacgatc ctcttttgcg
    52141 tcaggtactt acctcgcaag atgagtttgt cgtcgcgtcc ggattctcca ccgacccgct
    52201 ggaagaacag cacagcgtag tgcctggttt gttgcataaa taccacaacc gggcgctttt
    52261 gctggtcaaa ggcggctgcg cggtaaattg ccgctattgc ttccgtcgcc acttccccta
    52321 tgccgaaaat cagggcaaca agcgtaactg gcagactgct cttgagtatg ttgctgcgca
    52381 tccggaactg gacgagatga ttttctccgg cggcgatccg ctgatggcga aagatcacga
    52441 gctggactgg ttgctcacac aactggaagc catcccgcat atcaaacgtc tgcggattca
    52501 cagccgtctg ccgattgtga tcccggcacg tatcaccgag gcgctggttg aacgctttgc
    52561 ccgttctacg ctgcaaatct tgctggtgaa tcacatcaat catgccaatg aggtagatga
    52621 aacgttccgt caggcgatgg ctaaattgcg ccgtgtcggt gtcaccctgc ttaaccagag
    52681 cgttctgtta cgtggtgtga acgataacgc acaaacgctg gcaaacctga gtaatgcgtt
    52741 gttcgatgcc ggcgtaatgc cgtattacct gcatgtgctc gataaagtac agggcgcggc
    52801 gcattttatg gtgagtgatg acgaagcacg gcagattatg cgtgagttgc tgacactggt
    52861 gtcgggttat ctggtgccga aactggcgcg agaaatcggc ggcgaaccca gcaaaacgcc
    52921 gctggatctc cagctacgcc agcagtaact gattaaacag ggaagttaaa cattagcctg
    52981 atgcgctacg cttatcaggc ctacattgtg cattgaaatt gaatggattt gcaatgaact
    53041 gtaggccgga taaggcgttt acgccgcatc cggcaagagc aacaaacgca agtacagcaa
    53101 cctgtcatta aactgggaaa tttaatcttt gcccgttgca taacctttaa ttattcatat
    53161 acattgctgt tgttactctt tatatagcca acaccaataa ttaac
//
LOCUS       DAZFWL020000018        51166 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFWL020000018 DAZFWL020000000
VERSION     DAZFWL020000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 51166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.018"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            135..224
                     /gene="kdpF"
                     /locus_tag="ACHKNP_RS05930"
                     /old_locus_tag="pgaptmp_001186"
     CDS             135..224
                     /gene="kdpF"
                     /locus_tag="ACHKNP_RS05930"
                     /old_locus_tag="pgaptmp_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HGU4703392.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            224..1897
                     /gene="kdpA"
                     /locus_tag="ACHKNP_RS05935"
                     /old_locus_tag="pgaptmp_001187"
     CDS             224..1897
                     /gene="kdpA"
                     /locus_tag="ACHKNP_RS05935"
                     /old_locus_tag="pgaptmp_001187"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HGU4703393.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLDQHYLPLNPQQLPGLSWDLA
                     LNAAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGSVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1920..3968
                     /gene="kdpB"
                     /locus_tag="ACHKNP_RS05940"
                     /old_locus_tag="pgaptmp_001188"
     CDS             1920..3968
                     /gene="kdpB"
                     /locus_tag="ACHKNP_RS05940"
                     /old_locus_tag="pgaptmp_001188"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HGU4703394.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSARGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3977..4549
                     /gene="kdpC"
                     /locus_tag="ACHKNP_RS05945"
                     /old_locus_tag="pgaptmp_001189"
     CDS             3977..4549
                     /gene="kdpC"
                     /locus_tag="ACHKNP_RS05945"
                     /old_locus_tag="pgaptmp_001189"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HGU4703395.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4542..7226
                     /gene="kdpD"
                     /locus_tag="ACHKNP_RS05950"
                     /old_locus_tag="pgaptmp_001190"
     CDS             4542..7226
                     /gene="kdpD"
                     /locus_tag="ACHKNP_RS05950"
                     /old_locus_tag="pgaptmp_001190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HGU4703396.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLTDPAEEKAVVRYARE
                     HNLGKIILGRPASCRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7223..7900
                     /gene="kdpE"
                     /locus_tag="ACHKNP_RS05955"
                     /old_locus_tag="pgaptmp_001191"
     CDS             7223..7900
                     /gene="kdpE"
                     /locus_tag="ACHKNP_RS05955"
                     /old_locus_tag="pgaptmp_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HGU4703397.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
     gene            8062..8196
                     /locus_tag="ACHKNP_RS05960"
                     /old_locus_tag="pgaptmp_001192"
     CDS             8062..8196
                     /locus_tag="ACHKNP_RS05960"
                     /old_locus_tag="pgaptmp_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU4703398.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            8175..8279
                     /gene="speFL"
                     /locus_tag="ACHKNP_RS05965"
                     /old_locus_tag="pgaptmp_001193"
     CDS             8175..8279
                     /gene="speFL"
                     /locus_tag="ACHKNP_RS05965"
                     /old_locus_tag="pgaptmp_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HGU4703399.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            8590..10788
                     /gene="speF"
                     /locus_tag="ACHKNP_RS05970"
                     /old_locus_tag="pgaptmp_001194"
     CDS             8590..10788
                     /gene="speF"
                     /locus_tag="ACHKNP_RS05970"
                     /old_locus_tag="pgaptmp_001194"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HGU4703400.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            10785..12104
                     /gene="potE"
                     /locus_tag="ACHKNP_RS05975"
                     /old_locus_tag="pgaptmp_001195"
     CDS             10785..12104
                     /gene="potE"
                     /locus_tag="ACHKNP_RS05975"
                     /old_locus_tag="pgaptmp_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HGU4703401.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12160..13800)
                     /gene="pgm"
                     /locus_tag="ACHKNP_RS05980"
                     /old_locus_tag="pgaptmp_001196"
     CDS             complement(12160..13800)
                     /gene="pgm"
                     /locus_tag="ACHKNP_RS05980"
                     /old_locus_tag="pgaptmp_001196"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HGU4703402.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGT
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(13826..14371)
                     /gene="seqA"
                     /locus_tag="ACHKNP_RS05985"
                     /old_locus_tag="pgaptmp_001197"
     CDS             complement(13826..14371)
                     /gene="seqA"
                     /locus_tag="ACHKNP_RS05985"
                     /old_locus_tag="pgaptmp_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HGU4703403.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            14556..15320
                     /gene="ybfF"
                     /locus_tag="ACHKNP_RS05990"
                     /old_locus_tag="pgaptmp_001198"
     CDS             14556..15320
                     /gene="ybfF"
                     /locus_tag="ACHKNP_RS05990"
                     /old_locus_tag="pgaptmp_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4703404.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            15460..15753
                     /gene="ybfE"
                     /locus_tag="ACHKNP_RS05995"
                     /old_locus_tag="pgaptmp_001199"
     CDS             15460..15753
                     /gene="ybfE"
                     /locus_tag="ACHKNP_RS05995"
                     /old_locus_tag="pgaptmp_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HGU4703405.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            15893..16423
                     /gene="fldA"
                     /locus_tag="ACHKNP_RS06000"
                     /old_locus_tag="pgaptmp_001200"
     CDS             15893..16423
                     /gene="fldA"
                     /locus_tag="ACHKNP_RS06000"
                     /old_locus_tag="pgaptmp_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HGU4703406.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            16633..16719
                     /gene="uof"
                     /locus_tag="ACHKNP_RS06005"
                     /old_locus_tag="pgaptmp_001201"
     CDS             16633..16719
                     /gene="uof"
                     /locus_tag="ACHKNP_RS06005"
                     /old_locus_tag="pgaptmp_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HGU4703407.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            16712..17158
                     /gene="fur"
                     /locus_tag="ACHKNP_RS06010"
                     /old_locus_tag="pgaptmp_001202"
     CDS             16712..17158
                     /gene="fur"
                     /locus_tag="ACHKNP_RS06010"
                     /old_locus_tag="pgaptmp_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HGU4703408.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17242..17568)
                     /gene="chiQ"
                     /locus_tag="ACHKNP_RS06015"
                     /old_locus_tag="pgaptmp_001203"
     CDS             complement(17242..17568)
                     /gene="chiQ"
                     /locus_tag="ACHKNP_RS06015"
                     /old_locus_tag="pgaptmp_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HGU4703409.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(17618..19024)
                     /gene="chiP"
                     /locus_tag="ACHKNP_RS06020"
                     /old_locus_tag="pgaptmp_001204"
     CDS             complement(17618..19024)
                     /gene="chiP"
                     /locus_tag="ACHKNP_RS06020"
                     /old_locus_tag="pgaptmp_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HGU4703410.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(19470..21134)
                     /gene="glnS"
                     /locus_tag="ACHKNP_RS06025"
                     /old_locus_tag="pgaptmp_001205"
     CDS             complement(19470..21134)
                     /gene="glnS"
                     /locus_tag="ACHKNP_RS06025"
                     /old_locus_tag="pgaptmp_001205"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HGU4703411.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(21337..23283)
                     /gene="nagE"
                     /locus_tag="ACHKNP_RS06030"
                     /old_locus_tag="pgaptmp_001206"
     CDS             complement(21337..23283)
                     /gene="nagE"
                     /locus_tag="ACHKNP_RS06030"
                     /old_locus_tag="pgaptmp_001206"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HGU4703412.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            23616..24416
                     /gene="nagB"
                     /locus_tag="ACHKNP_RS06035"
                     /old_locus_tag="pgaptmp_001207"
     CDS             23616..24416
                     /gene="nagB"
                     /locus_tag="ACHKNP_RS06035"
                     /old_locus_tag="pgaptmp_001207"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGU4703413.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            24476..25624
                     /gene="nagA"
                     /locus_tag="ACHKNP_RS06040"
                     /old_locus_tag="pgaptmp_001208"
     CDS             24476..25624
                     /gene="nagA"
                     /locus_tag="ACHKNP_RS06040"
                     /old_locus_tag="pgaptmp_001208"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU4703414.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            25633..26853
                     /gene="nagC"
                     /locus_tag="ACHKNP_RS06045"
                     /old_locus_tag="pgaptmp_001209"
                     /gene_synonym="nagR"
     CDS             25633..26853
                     /gene="nagC"
                     /locus_tag="ACHKNP_RS06045"
                     /old_locus_tag="pgaptmp_001209"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HGU4703415.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            26901..27653
                     /gene="nagD"
                     /locus_tag="ACHKNP_RS06050"
                     /old_locus_tag="pgaptmp_001210"
     CDS             26901..27653
                     /gene="nagD"
                     /locus_tag="ACHKNP_RS06050"
                     /old_locus_tag="pgaptmp_001210"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HGU4703416.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            27910..29574
                     /gene="asnB"
                     /locus_tag="ACHKNP_RS06055"
                     /old_locus_tag="pgaptmp_001211"
     CDS             27910..29574
                     /gene="asnB"
                     /locus_tag="ACHKNP_RS06055"
                     /old_locus_tag="pgaptmp_001211"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HGU4703417.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            29955..30031
                     /locus_tag="ACHKNP_RS06060"
                     /old_locus_tag="pgaptmp_001212"
     tRNA            29955..30031
                     /locus_tag="ACHKNP_RS06060"
                     /old_locus_tag="pgaptmp_001212"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29989..29991,aa:Met,seq:cat)
     gene            30040..30124
                     /locus_tag="ACHKNP_RS06065"
                     /old_locus_tag="pgaptmp_001213"
     tRNA            30040..30124
                     /locus_tag="ACHKNP_RS06065"
                     /old_locus_tag="pgaptmp_001213"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30074..30076,aa:Leu,seq:tag)
     gene            30148..30222
                     /locus_tag="ACHKNP_RS06070"
                     /old_locus_tag="pgaptmp_001214"
     tRNA            30148..30222
                     /locus_tag="ACHKNP_RS06070"
                     /old_locus_tag="pgaptmp_001214"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30180..30182,aa:Gln,seq:ttg)
     gene            30257..30331
                     /locus_tag="ACHKNP_RS06075"
                     /old_locus_tag="pgaptmp_001215"
     tRNA            30257..30331
                     /locus_tag="ACHKNP_RS06075"
                     /old_locus_tag="pgaptmp_001215"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30289..30291,aa:Gln,seq:ttg)
     gene            30347..30423
                     /locus_tag="ACHKNP_RS06080"
                     /old_locus_tag="pgaptmp_001216"
     tRNA            30347..30423
                     /locus_tag="ACHKNP_RS06080"
                     /old_locus_tag="pgaptmp_001216"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30381..30383,aa:Met,seq:cat)
     gene            30472..30546
                     /locus_tag="ACHKNP_RS06085"
                     /old_locus_tag="pgaptmp_001217"
     tRNA            30472..30546
                     /locus_tag="ACHKNP_RS06085"
                     /old_locus_tag="pgaptmp_001217"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30504..30506,aa:Gln,seq:ctg)
     gene            30584..30658
                     /locus_tag="ACHKNP_RS06090"
                     /old_locus_tag="pgaptmp_001218"
     tRNA            30584..30658
                     /locus_tag="ACHKNP_RS06090"
                     /old_locus_tag="pgaptmp_001218"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30616..30618,aa:Gln,seq:ctg)
     gene            complement(30812..31987)
                     /gene="ubiF"
                     /locus_tag="ACHKNP_RS06095"
                     /old_locus_tag="pgaptmp_001219"
     CDS             complement(30812..31987)
                     /gene="ubiF"
                     /locus_tag="ACHKNP_RS06095"
                     /old_locus_tag="pgaptmp_001219"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HGU4703418.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGGNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            32133..33557
                     /gene="miaB"
                     /locus_tag="ACHKNP_RS06100"
                     /old_locus_tag="pgaptmp_001220"
     CDS             32133..33557
                     /gene="miaB"
                     /locus_tag="ACHKNP_RS06100"
                     /old_locus_tag="pgaptmp_001220"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HGU4703419.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            33710..34750
                     /gene="ybeZ"
                     /locus_tag="ACHKNP_RS06105"
                     /old_locus_tag="pgaptmp_001221"
     CDS             33710..34750
                     /gene="ybeZ"
                     /locus_tag="ACHKNP_RS06105"
                     /old_locus_tag="pgaptmp_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGU4703420.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            34747..35214
                     /gene="ybeY"
                     /locus_tag="ACHKNP_RS06110"
                     /old_locus_tag="pgaptmp_001222"
     CDS             34747..35214
                     /gene="ybeY"
                     /locus_tag="ACHKNP_RS06110"
                     /old_locus_tag="pgaptmp_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGU4703421.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            35304..36182
                     /gene="corC"
                     /locus_tag="ACHKNP_RS06115"
                     /old_locus_tag="pgaptmp_001223"
     CDS             35304..36182
                     /gene="corC"
                     /locus_tag="ACHKNP_RS06115"
                     /old_locus_tag="pgaptmp_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HGU4703422.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            36207..37745
                     /gene="lnt"
                     /locus_tag="ACHKNP_RS06120"
                     /old_locus_tag="pgaptmp_001224"
     CDS             36207..37745
                     /gene="lnt"
                     /locus_tag="ACHKNP_RS06120"
                     /old_locus_tag="pgaptmp_001224"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HGU4703423.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGTGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(37823..39700)
                     /locus_tag="ACHKNP_RS06125"
                     /old_locus_tag="pgaptmp_001225"
     CDS             complement(37823..39700)
                     /locus_tag="ACHKNP_RS06125"
                     /old_locus_tag="pgaptmp_001225"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HGU4703424.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSFILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            40155..41063
                     /gene="gltI"
                     /locus_tag="ACHKNP_RS06130"
                     /old_locus_tag="pgaptmp_001226"
     CDS             40155..41063
                     /gene="gltI"
                     /locus_tag="ACHKNP_RS06130"
                     /old_locus_tag="pgaptmp_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HGU4703425.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            41233..41973
                     /gene="gltJ"
                     /locus_tag="ACHKNP_RS06135"
                     /old_locus_tag="pgaptmp_001227"
     CDS             41233..41973
                     /gene="gltJ"
                     /locus_tag="ACHKNP_RS06135"
                     /old_locus_tag="pgaptmp_001227"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HGU4703426.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            41973..42647
                     /gene="gltK"
                     /locus_tag="ACHKNP_RS06140"
                     /old_locus_tag="pgaptmp_001228"
     CDS             41973..42647
                     /gene="gltK"
                     /locus_tag="ACHKNP_RS06140"
                     /old_locus_tag="pgaptmp_001228"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HGU4703427.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            42647..43372
                     /gene="gltL"
                     /locus_tag="ACHKNP_RS06145"
                     /old_locus_tag="pgaptmp_001229"
     CDS             42647..43372
                     /gene="gltL"
                     /locus_tag="ACHKNP_RS06145"
                     /old_locus_tag="pgaptmp_001229"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HGU4703428.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            43490..44425
                     /gene="rihA"
                     /locus_tag="ACHKNP_RS06150"
                     /old_locus_tag="pgaptmp_001230"
     CDS             43490..44425
                     /gene="rihA"
                     /locus_tag="ACHKNP_RS06150"
                     /old_locus_tag="pgaptmp_001230"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HGU4703429.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            44509..46179
                     /gene="hscC"
                     /locus_tag="ACHKNP_RS06155"
                     /old_locus_tag="pgaptmp_001231"
     CDS             44509..46179
                     /gene="hscC"
                     /locus_tag="ACHKNP_RS06155"
                     /old_locus_tag="pgaptmp_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HGU4703430.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELGALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQNSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(46239..47690)
                     /gene="djlC"
                     /locus_tag="ACHKNP_RS06160"
                     /old_locus_tag="pgaptmp_001232"
     CDS             complement(46239..47690)
                     /gene="djlC"
                     /locus_tag="ACHKNP_RS06160"
                     /old_locus_tag="pgaptmp_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HGU4703431.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGLWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(47687..48394)
                     /gene="ybeU"
                     /locus_tag="ACHKNP_RS06165"
                     /old_locus_tag="pgaptmp_001233"
     CDS             complement(47687..48394)
                     /gene="ybeU"
                     /locus_tag="ACHKNP_RS06165"
                     /old_locus_tag="pgaptmp_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4703432.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSCVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            48495..49049
                     /gene="ybeT"
                     /locus_tag="ACHKNP_RS06170"
                     /old_locus_tag="pgaptmp_001234"
     CDS             48495..49049
                     /gene="ybeT"
                     /locus_tag="ACHKNP_RS06170"
                     /old_locus_tag="pgaptmp_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4703433.1"
                     /translation="MNKKLMYIFAIFIVAAITCISHPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(49059..50486)
                     /gene="djlB"
                     /locus_tag="ACHKNP_RS06175"
                     /old_locus_tag="pgaptmp_001235"
     CDS             complement(49059..50486)
                     /gene="djlB"
                     /locus_tag="ACHKNP_RS06175"
                     /old_locus_tag="pgaptmp_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HGU4703434.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(50483..>51166)
                     /gene="ybeR"
                     /locus_tag="ACHKNP_RS06180"
                     /old_locus_tag="pgaptmp_001236"
     CDS             complement(50483..>51166)
                     /gene="ybeR"
                     /locus_tag="ACHKNP_RS06180"
                     /old_locus_tag="pgaptmp_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4703435.1"
                     /translation="LFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSSWGIDARDD
                     LLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFVASTAECCG
                     EGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQYFAGYTFG
                     RQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQPLAYYSACP
                     ETLKDMSDL"
BASE COUNT        12351 a        13059 c        13142 g        12614 t
ORIGIN      
        1 tactaaacat tagttaaatc atggcttttg ccatttttat acttttttta caccccgccc
       61 gcagattttt gcgaaatctt tgcagccaga attctaccct tccggtatca cttttaggcc
      121 actggaggtg cactgtgagt gcaggcgtga taaccggcgt attgctggtg tttttattac
      181 tgggttatct ggtttatgcc ctgatcaatg cggaggcgtt ctgatggctg cgcaagggtt
      241 cttactgatc gccacgtttt tactggtgtt aatggtgctg gcgcgtcctt taggcagcgg
      301 gctggcgcgg ctgattaatg acattcctct tccaggtaca gcgggcgttg aacgtatact
      361 ctttcgttta cctggcgtct ctgaccatga gatgaactgg aagcaatatc tttgtgccat
      421 tctcggcctg aacatgctgg ggctggcggt gctgtttttt atgttgctcg atcagcacta
      481 tctgccgctt aatccacagc agttgccggg gctgtcgtgg gatctggcgc tgaatgccgc
      541 cgtcagcttt gtcacgaata ccaactggca atcttacagc ggtgaaacca cgttgagcta
      601 tttcagccag atggctggct taacggtgca aaactttctt tctgccgcca gcgggattgc
      661 ggtgattttt gccctcatcc gtgcgtttac ccgccagagc atgagcacac tcgggaatgc
      721 ctgggtcgat ctgctacgca tcacgttatg ggtgctcgtc cctgtggcgt tgttgatcgc
      781 actgtttttt attcaacaag gtgcgctgca aaactttcag ccttatcagg ctgtgaatac
      841 cgttgaagga gcgcaacagc tgttacccat ggggcctgta gcttctcagg aagcgatcaa
      901 gatgctcggt actaacggcg gtggcttctt taatgccaac tcgtcgcatc cgtttgaaaa
      961 cccaaccgca ctgaccaact tcgtgcagat gctggcgatc ttcttgatcc caacggcgct
     1021 gtgctttgcc tttggtgaag tgacgggcga tcgccgccag gggcgcatgt tgctgtgggc
     1081 gatgtcagtg atttttgtca tctgcgtagg cgtggtgatg tgggcagaag ttcagggtaa
     1141 tcctcatctg ctggcgctcg gcgcagacag cagcatcaat atggaaggta aagagagccg
     1201 tttcggcgta ctggtcagta gcctgtttgc ggtcgtgact acggcggctt cctgtggcgc
     1261 ggtgattgcg atgcatgatt cgtttaccgc tctcggtggc atggtgccga tgtggctgat
     1321 gcaaattggt gaagtggtgt tcggcagtgt cggttctggt ctttacggca tgatgctgtt
     1381 tgtcctgctg gcggtgttta ttgccgggct gatgattggt cgtacaccgg aatatctggg
     1441 taaaaaaatc gacgtacgcg agatgaaact gactgcactg gcgattctgg ttaccccgac
     1501 gctggtgctg atgggcgcgg cgctggcaat gatgaccgac gccggacgta gcgccatgct
     1561 caaccctggc ccgcatggtt ttagcgaagt gctgtacgcc gtgtcatccg ccgctaacaa
     1621 caacggcagc gcctttgccg gattaagcgc caactccccg ttctggaact gtttactggc
     1681 gttctgcatg tttgtcggtc gcttcggggt gattatcccg gtgatggcaa ttgccggttc
     1741 gctggtgagt aaaaagagcc aacccgccag ctccggcacg ctgccaacgc acggcccgct
     1801 gtttgttggc ctgttaatcg gcaccgtgtt gctggttggc gcactgacct ttatccctgc
     1861 cctggcgctt ggtccggtgg cggaatatct ctcctgatga tattgagtga gcactgaata
     1921 tgagtcgtaa acaactggcg ctattcgaac caacacttgt cgttcaggcg ctgaaagaag
     1981 cggtgaaaaa attaaacccg caggcgcaat ggcgcaatcc ggtgatgttt atcgtctgga
     2041 tcggcagtct gctgaccacc tgtattagca tcgcgatggc aagcggtgcg atgcctggca
     2101 atgcgctgtt tagcgcggca attagcggct ggctgtgggt caccgtactg ttcgctaatt
     2161 tcgccgaggc gctggcagaa ggccgcagta aagcgcaggc caacagtctt aaaggggtga
     2221 aaaaaactgc ctttgcccgc aagctgcgtg agccgaaata tggcgctgcg gcggacaaag
     2281 ttcctgccga tcaactgcgt aaaggtgaca tcgtgctggt agaagctggc gatattatcc
     2341 cctgcgatgg tgaagtcatt gaaggcggcg catcggtcga tgaaagcgcc atcaccgggg
     2401 aatcggcacc ggtgatccgt gaatccggcg gcgattttgc ctccgtcacc ggcggcacgc
     2461 gtattctttc tgactggctg gtgattgagt gtagcgttaa ccccggtgag acatttctgg
     2521 atcggatgat cgcgatggtg gaaggcgcac agcgacgcaa aacaccgaac gagattgccc
     2581 tgactatcct gctgattgct ctgaccatcg tctttttact ggcaaccgcc acgctgtggc
     2641 cgttttccgc gaggggcggt aatgccgtga gtgtaacggt actggtggcg ctgctggtct
     2701 gtctgatccc aaccactatt ggcggcctgt tgtcagcgat cggcgtcgcc gggatgagtc
     2761 ggatgctggg cgcgaatgtg attgccacca gcggacgtgc agttgaagcg gcaggtgacg
     2821 ttgacgttct gctactggat aaaaccggca ccatcacact cggtaaccgt caggcgtcgg
     2881 agtttatccc tgcgcagggc gtggatgaaa aaacgctggc tgacgccgca caactggctt
     2941 cgctggctga tgaaacgccg gaaggccgca gtattgtgat cctcgccaag caacgtttta
     3001 acctgcgcga gcgcgatgtg cagtcgctcc atgccacctt tgtaccgttt actgcgcaaa
     3061 gccggatgag cgggatcaac atcgacaacc gcatgatccg taaaggttct gtcgatgcca
     3121 ttcgccgcca tgttgaggct aacggcggtc acttccctac cgatgttgat caaaaagtcg
     3181 atcaggttgc gcgtcagggg gccacgccgc tggtggtggt ggaaggttcc cgtgtgctgg
     3241 gcgttattgc gctgaaagat atcgtcaaag gcggtattaa agagcgcttc gcccagctgc
     3301 gcaaaatggg cattaaaacg gtgatgatta ccggcgataa ccgtctgact gccgccgcca
     3361 ttgctgcgga agcgggcgtc gatgattttc tcgccgaagc gacaccggag gccaagctgg
     3421 cattgattcg tcagtatcag gcggaaggcc gtttggtggc gatgaccggc gacggcacca
     3481 acgatgctcc agcgctggcg caggcagatg tcgcggtggc gatgaactcc ggtactcagg
     3541 cggcgaaaga agcgggcaat atggtcgatc tcgactctaa cccgaccaag ttgatcgagg
     3601 tggtacatat tggcaaacag atgctgatga cccgtggctc gctgaccacc ttcagcattg
     3661 ccaacgatgt ggcgaaatac ttcgccatta ttccggcggc gttcgcggca acgtatccgc
     3721 agttaaatgc gctgaacatc atgcgccttc attcgcccga ctccgccatt ctcagtgcgg
     3781 tcattttcaa cgccttgatt atcgtctttt tgattcctct ggcgttaaaa ggcgtgagtt
     3841 ataaaccgct taccgcttct gccatgttgc gccgtaactt atggatttac ggtctgggtg
     3901 ggctgctggt gccgtttatt ggtatcaaag tcattgattt actgctgacc gtttgcggtc
     3961 tggtgtgagg tttaccatgc gtggcttacg tccggcatta tcgacattta tctttctgtt
     4021 attgattact ggcggcgttt acccgctgct gaccaccgtg ctggggcaat ggtggtttcc
     4081 ctggcaggcc aacggttcgt tgattcgtga aggtgatacg gtgcggggtt cggcattaat
     4141 cgggcagaat tttaccggca acggctattt tcagggtcgc ccgtcggcaa cggcagaaat
     4201 gccctataat ccacaggctt ctggcgggag caatctggcg gtcagtaacc ctgagctgga
     4261 taaacaaata gccgcacgcg ttgctgcatt acgggccgct aacccggatg ccagtacgaa
     4321 cgttccggtt gaactggtga cggcatcggc aagcgggctg gacaataata tcaccccgca
     4381 agcggcggtc tggcaaatcc cacgcgtggc gaaagcgcgt aatctcagcg ttgaacagct
     4441 cacgcaactg atcgcaaaat acagccaaca accgctggtg aaatatatcg gccagccggt
     4501 cgtcaacatt gttgaactca atctggcgct ggataaactt gatgaataac gaacccttac
     4561 gtcccgaccc cgatcgtctg ctggaacaaa ctgccgcgcc gcatcggggg aagctgaaag
     4621 ttttcttcgg tgcctgtgca ggcgtcggga agacctgggc gatgctggca gaagcccagc
     4681 gactgcgggc gcaagggctg gatattgtgg ttggcgtggt agaaacccat gggcggaaag
     4741 ataccgccgc catgcttgaa gggctggctg ttctgccgcc caaacgccag gcgtaccgtg
     4801 ggcggcatat cagcgagttt gatctcgatg ccgccctcgc ccgccgtccg gcgctgatct
     4861 taatggacga actggcgcac agtaatgcgc caggttctcg tcatcctaaa cgctggcagg
     4921 atatcgaaga actgctggaa gctggcattg atgttttcac aaccgtcaac gttcagcatc
     4981 tggaaagtct gaatgatgtg gtcagcggcg tcaccggaat tcaggtacgg gaaaccgtgc
     5041 ccgatccttt tttcgatgcc gccgacgacg tagtgctggt ggacttgccc ccggacgatc
     5101 tgcgccagcg gctgaaagaa ggcaaagtct atattgccgg gcaggcggag cgcgccattg
     5161 aacatttttt ccgcaaaggc aatctgatcg ccctgcgtga actggcactg cgccgtactg
     5221 ccgatcgcgt agatgagcaa atgcgcgcct ggcgggggca tcctggtgaa gagaaagtgt
     5281 ggcacacgcg cgacgcgatc cttttatgca tcgggcataa caccggcagt gagaaactgg
     5341 tccgcgcggc ggcgcggttg gcctcgcgcc tgggtagcgt ctggcacgcg gtgtatgttg
     5401 aaacccctgc cctgcatcgc ttaccggaaa aaaaacgtcg ggcaattctc agcgccttac
     5461 gtctggcgca ggagctgggc gcggagacgg ctacacttac tgatccagcg gaagaaaaag
     5521 cagtagtgcg ttatgcccgt gaacataatc tcggcaagat tattctcggt cgcccggcct
     5581 cgtgccgctg gtggcgtcgg gaaacgtttg ctgaccgact ggcgcgcatc gcccccgatc
     5641 tcgatcaggt gctggtcgcg cttgatgaac cacccgcccg cacgattaac aacgcgccgg
     5701 atagccgctc ttttaaagac aagtggcggg tacagattca gggatgcgtg gttgccgccg
     5761 cgttatgcgc cgttatcact ttaattgcca tgcagtggct gatggcgttt gatgccgcca
     5821 acctggtgat gctgtatctg cttggcgtgg tggtggtggc gctattttat ggacgctggc
     5881 cttcggtggt tgctaccgtc attaatgtgg tgagtttcga tctctttttt atcgccccac
     5941 gcggcacgct cgctgtctct gatgtgcaat atctgctgac cttcgcggtg atgttaaccg
     6001 tcgggctggt gatcgggaac cttactgcgg gtgtgcgtta tcaggcgcgg gtagcccgtt
     6061 accgcgagca acgcacacgg cacttatatg aaatgtcgaa agctctggcg gtgggccgca
     6121 gtccgcagga catcgccgcc accagcgaac aatttattgc ctccacgttt catgcccgca
     6181 gtcaggtgtt gttgcccgat gacaacggta aattgcagcc gttaacacat ccgcaaggaa
     6241 tgacgccatg ggacgatgcc atcgcgcagt ggagttttga taaaggcctg cctgcgggtg
     6301 cgggcaccga cacgttaccc ggcgtgccgt accagatttt gccgctaaaa agcggcgaga
     6361 aaacctacgg actggtggtg gtggaaccgg ggaatctgcg tcagttgatg atcccggagc
     6421 agcagcgcct gctagagacg tttacgctgt tagtcgccaa tgcgtttgag cggctgacgc
     6481 tgaccgccag tgaagaacag gcgcggatgg caagcgaacg tgaacagatc cgcaacgccc
     6541 tgctggcggc gctttcgcat gatttacgca cgccgcttac ggtgctgttt ggtcaggcag
     6601 aaatcttaac gctcgatctg gcaagcgaag gatcgcccca cgcccgccag gccagcgaga
     6661 ttcgtcagca tgtgctgaac actacccgac tggtgaataa tctactggat atggcgcgaa
     6721 ttcagtccgg cggctttaat ttgaagaaag agtggttaac gctggaagaa gtagtcggca
     6781 gcgctctgca aatgctggaa ccgggtttat cgtcgcccat caatctttct ctgccagaac
     6841 cgctgacctt aatccacgtt gacgggccac tctttgaacg ggtgctgatt aatctgctgg
     6901 agaacgcggt gaaatatgcg ggtgcgcagg ccgaaattgg tatcgatgcc cacgttgagg
     6961 gcgaaaatct acaactggat gtctgggata acggccccgg tcttccgcca ggccaggagc
     7021 agacgatatt tgataagttt gctcgcggga ataaagagtc ggcagtaccg ggggtagggc
     7081 ttggactggc aatttgtcgg gcgatagtgg atgtacacgg gggcactatt accgcgttca
     7141 accgaccgga aggtggtgcc tgttttcgtg ttacacttcc ccagcaaacc gctcctgaac
     7201 ttgaagactt tcatgaggat atgtgacaaa cgttctgatt gttgaagatg aacaggctat
     7261 tcgtcgcttt ctgcgcacgg cgctggaggg cgacgggatg cgcgtctatg aggccgaaac
     7321 gctgcaacgc ggcttgctgg aagcggctac ccgtaagcca gatttgatta ttctcgatct
     7381 cggcctgccc gatggtgatg ggattgagtt tatccgcgac ctgcgccagt ggagcgcggt
     7441 gccggtgatt gtgctttccg cacgcagcga agagagcgac aaaatcgccg cgctggatgc
     7501 cggagcggat gattatctga gtaagccgtt tggcattggc gaattgcagg cccgtctgcg
     7561 cgtcgcttta cgccgccact ccgccaccgc cgcgcccgat ccgctggtga aattttccga
     7621 tgttaccgtc gatttagccg cccgcgtgat tcaccggggt gaggaagagg tgcatctcac
     7681 gccgattgag ttccgcctgc tggcggtgct gctaaataat gccgggaaag tgctcaccca
     7741 gcgccagtta ctcaatcagg tgtgggggcc aaacgcggtc gaacacagtc actatttgcg
     7801 tatttatatg ggacatctgc gacaaaaact ggaacaggat cccgctcgcc cccgccattt
     7861 cattactgaa accggtattg ggtatcggtt tatgccatga atgtttattc aataaatgcg
     7921 cttaaataat tattaattta ttattaaacc tgccaaaaat attattattt ggcaggttta
     7981 atttcttaac gcaaatataa aacacaaaat tacaacattt aaataacaaa acagcatcta
     8041 atatggcgct attcataatt aatgatttat tatttggggt atgatcgttt tttgttgatc
     8101 ttcttcacag attatagcca tttcatggat agaataactc taccttcaac tgacacagca
     8161 agaggtaaag gtaaatggaa aataataacc gcttaatgcc tcatataagg cggacaaccc
     8221 atatcatgaa gtttgcccat cgtaatagtt ttgactttca tttctttaat gcccgctagt
     8281 cttctgacta aagggcaccc caacgtacag gtctccctga ctttaagcat tacaggttaa
     8341 tacctgtatt cctcggtgct catatactgc taaccctttt aaactctaaa taattcgagt
     8401 cgcagcactt gcaacttgag gtatgacgag tatagccagt taccgggctg gtctgggtta
     8461 ttgcatctgc aaaaagcaaa ctactgattt atttatcagc ggtggagctt tgcttttttt
     8521 ccggcgtgat cgatttctcc tttgagaaat tgaggacctg ctattacctg aaataaagag
     8581 atgaacaaaa tgtcagaatt aaaaattgcc gttagtcgtt cttgcccgga ttgtttttcc
     8641 actcatcgtg catgcgtgaa tatagacgaa agtaattata ttgacgttgc cgccattatt
     8701 ttatcagtca gtgatgttga acgtggaaaa ctcgatgaaa tagacgctac tggctatgac
     8761 attcctgttt ttattgcaac ggaaaatgaa gaacgtgtac ccgcagaata tctgccacgt
     8821 atttctggtg tcttcgaaca ttgcgaatca cgtaaagagt tttatggacg tcagttagaa
     8881 accgctgcca gccattatga aacgcaactg cgcccgccct tcttccgcgc actggtcgat
     8941 tatgttaatc agggcaatag tgcttttgac tgccctggtc atcagggggg tgagtttttc
     9001 cgtcgccatc cggcaggcaa tcagttcgtg gaatactttg gcgaaatgct cttccgctct
     9061 gacttgtgta atgctgacgt agcaatgggc gatctgctga ttcatgaagg tgcgccatgt
     9121 attgcacaac agcatgcggc taaagtcttt aatgccgata aaacctattt cgttttaaac
     9181 ggtacttcat cttctaataa agtggtctta aacgcattgc tgacgccggg cgatctggtg
     9241 ttgtttgacc gcaataacca taaatctaac caccacggtg cgttgctaca ggctggcgcg
     9301 acaccggttt atctggaaac cgcacgtaac ccgtatggct ttatcggtgg cattgatgcg
     9361 cactgttttg aagaaagtta cctgcgtgag ctgatcgcgg aagtcgcacc gcagcgggca
     9421 aaagaggctc gtcctttccg cctcgctgtg attcagttag gcacctacga cggtacgatt
     9481 tataacgccc gccaggtggt ggataaaatt ggtcatctgt gtgactacat tctgtttgac
     9541 tccgcatggg ttggctatga gcaatttatc ccgatgatgg ctgactgttc gccgctgttg
     9601 ctggatctta atgagaacga tccgggtatt ctggttacgc aatccgtaca taaacagcag
     9661 gctggttttt ctcaaacttc acaaatccat aaaaaagaca gccacatcaa agggcaacag
     9721 cgttatgtac cgcacaaacg catgaacaac gcctttatga tgcatgcctc caccagcccg
     9781 ttctatccgc tgtttgccgc actggatatc aacgccaaaa tgcatgaagg tgtcagcggt
     9841 cgtaatatgt ggatggattg tgtggtaaat ggcattaatg cccgcaagct gatcctcgat
     9901 aactgtcagc atattcgtcc gttcgtacct gaactggtgg atggtaaacc ctggcagtcg
     9961 tatgaaacag cgcaaattgc ggttgatctg cgcttcttcc agtttgtacc aggtgagcac
    10021 tggcactctt ttgaaggcta tgcagagaat caatactttg tcgatccatg caaactgttg
    10081 ctgacaaccc caggtattga tgcacgtaac ggcgaatatg aagcgttcgg tgtacccgcg
    10141 acgattcttg ctaacttcct gcgcgaaaat ggcgtagtgc cggaaaaatg cgatcttaac
    10201 tccatcctct tcctgctgac tccggcagaa gatatggcca agcttcagca acttgttgcc
    10261 ctgctggtac gcttcgaaaa actgcttgag tccgacgcgc cattagcaga agtgctgcct
    10321 tccatctaca aacagcatga agagcgctac gccggttata ccctgcgtca gttgtgtcaa
    10381 gaaatgcatg atttgtatgc ccgtcacaac gtgaaacaac tgcaaaaaga gatgttccgt
    10441 aaggaacact tcccacgcgt aagcatgaat ccgcaagaag ccaactacgc ctatttacgc
    10501 ggtgaagtgg agctggttcg tctgccggat gcagaaggcc gtatcgctgc cgaaggtgcg
    10561 cttccttatc ctccgggtgt gctgtgtgtt gttccgggtg aaatctgggg tggtgctgtt
    10621 ttgcgttact tcagcgctct ggaagaaggg atcaacctgc tgccaggttt tgcaccggag
    10681 ctgcagggtg tctatatcga agaacatgat ggtcgtaagc aagtttggtg ctatgtcatc
    10741 aagcctcgtg atgcgcaaag caccctgttg aaaggggaaa aattatgagt caggctaaat
    10801 cgaacaaaat gggcgtcgtt cagttaacca tactgacgat ggtcaacatg atgggctccg
    10861 gtatcatcat gctgccgaca aagcttgccg aagttgggac aatttcaatt atctcctggc
    10921 tggtgacagc cgtaggctca atggcactgg catgggcatt cgcaaaatgc ggtatgttca
    10981 gccgtaaatc aggcggtatg ggcggttatg cggagtatgc tttcggtaaa tccggtaact
    11041 ttatggcgaa ctatacctac ggcgtctcgc tgctgattgc taacgtcgcg attgctattt
    11101 cggcggttgg ttacggcacc gaactgctcg gcgcgagttt gtcgccagtg cagattggtc
    11161 ttgcgaccat cggggtgctg tggatttgta ccgtggctaa ctttggtggt gcgcgcatta
    11221 ccgggcaaat cagtagcatt accgtgtggg gggtcattat tccggtggta ggcctgtgca
    11281 ttatcggctg gttctggttt agcccgacgc tttacgttga ttcctggaat ccgcatcatg
    11341 caccgttctt cagtgcagta ggttcttcca tcgctatgac gctgtgggct tttctcggtc
    11401 tggagtctgc gtgtgcgaat actgatgtag tggaaaaccc ggaacgtaat gtgccaatcg
    11461 cggtactcgg cggtacgtta ggtgcggcgg tgatttatat cgtctccacc aacgtgattg
    11521 cagggattgt gcctaatatg gagctggcaa attcaacggc accatttggc ctggccttcg
    11581 cacagatgtt cacaccagaa gtgggtaaag tcattatggc gctgatggtg atgtcctgct
    11641 gcggttcgct gcttggctgg cagttcacca ttgcccaggt gtttaaatct tcatctgatg
    11701 aaggctactt ccctaaaatt ttctcccgtg taaccaaagt ggatgcaccg gtgcagggaa
    11761 tgttgaccat tgtgattatc cagagcgggt tggcgctgat gacgattagc ccgtcgctaa
    11821 acagtcagtt caacgtgctg gttaacctgg ccgtggtgac caatatcatt ccgtatattc
    11881 tgtcaatggc ggcattagtc attattcaga aggtcgctaa tgtgcccccc tcaaaagcga
    11941 aagttgcaaa ctttgttgct tttgttggcg cgatgtatag cttttatgcg ctgtactcat
    12001 ccggggaaga agccatgctg tacggttcca tcgtgacctt ccttggttgg acactgtatg
    12061 gtctggtatc accacgcttt gaactgaaaa ataaacacgg ttaaaaaagc aaagtaaaaa
    12121 agggcgatct tgcgaccgcc ctttttttat taaatgtgtt tacgcgtttt tcagaacttc
    12181 gctaacaatc tcaaccgctt ctttctcaat ctgcttgcga tgttcttcac cgaggaagct
    12241 ttcgcagtag atcttatatg cgtcttccgt gcctgacgga cgcgcggcga accagccgtt
    12301 gtcagtcatc actttcagac cgccgataga agcaccatta cccggtgccg ccgtcagacg
    12361 tgcggtgatc gggtcacctg ccagggtgct ggcgctcacc atttccggag acagcttaga
    12421 caacgccgct ttttgtgcgg aagtcgcagc ggcctgcaaa cggttgtagc tcggcgcacc
    12481 aaagcgtttt gccagttcgt tgtagtgttc ctgcgggttc ttaccggtga cagcggtgat
    12541 ttccgccgcc agcagacaca taatgatgcc gtctttatcg gtggaccacg gcgtgccgtc
    12601 gaaacgcagg aaggaagccc cggcgctctc ttcgccgcca aagccgaaac tgccgtcgaa
    12661 cagaccatca acaaaccatt tgaatcctac cgggacttct accagtttac ggcccaaggc
    12721 gttgaccaca cggtcgatca tcgcagaaga aaccagcgtt ttaccgacag caacatcttt
    12781 gccccactgc ggacgatgct ggaacaggta gttgatcgcc accgccaggt agtggttcgg
    12841 attcatcaaa cctgccggag tgacgatacc gtgacggtca taatccgggt cgttagcaaa
    12901 cgccagatcg aacttatcac gcagtgccag caggcccgcc atcgcacact cggaggagca
    12961 gtccatacgg atcgtgccgt ctttatcaag gtgcataaag cggaaggttt gatcgacctg
    13021 atcgttaacg atggtcaggt tgaggttgta atactcgcca atacgcttcc agtattcgat
    13081 accggaaccg cccagcggat caacgccaag cgtcaggccc gctttctgaa tcgccgccat
    13141 atcaacgata tcggccagac cttccacaaa cggctgcacc agatcctgct ctttcacatg
    13201 accggatgcc atcgcttcgt cgagagagat acgcttcacg cctttcaggc catcggccat
    13261 cagtgcgttg gccctgtctt ccaccacttt ggtgacgttg gtatcagccg ggccaccatt
    13321 tggcgggttg tacttgatac caccatcttc cggcgggtta tgggacggtg taatcacgat
    13381 accgtctgcc agcgggccac cttttttatt gtgaaccagg atggcattgg aaacggcagg
    13441 cgtcggagtg aatccattgt tttcctgcac aatgacatca acgccgttcg ctgccagcac
    13501 ttccagtacg gaaatgaacg caggctcgga cagggcgtga gtatctttac ccacatagca
    13561 agggccagtg atgccatttt tcgcacgttc ttcagcaatt gcctgagcga tcgccagaat
    13621 gtgcggctcg ttaaagctgt ggcgcgctgc actgccacgg tgaccggaag taccgaattt
    13681 caccgcgtgc tccgcattcc ctgcttctgg tttcagtaca taatattgcg ccgtcagttg
    13741 ggcgacgtta atcaaatcac tctgttgtgc aggttggcct gcacgattgt ggattgccat
    13801 tgctttgtcc tttgtctgca acgttttaga tagttccgcg aaccttctca atcaattccg
    13861 ccgggaattg catcgactgc atgatgtgtt cgatcatgct gcatttacgg ccggtgttgg
    13921 tgttggtgat cacccaatac ggcgtgcctg gcacatgttt cggcttggtc tgattaccat
    13981 ttttcagcag cgtttgttca tctgccgcaa agtaaacgcg tgtacgaccg tgcaacgatt
    14041 ccgttgcttc ggcaaacgcc tgggcgtcaa gagaatatag tgtagacaac agcagcataa
    14101 agcgattgac cgctcgcttt tgctctgcgt attcgtccga aagcagaagt tcacgcattg
    14161 cgcgaacctt gtctttaatc gttttgaccg gcttcgcttc gacgatagca ggtgacgcaa
    14221 cgcgaacctc tttcgtcacc ggagcagcag gctgtgatga ggcggaaaat ttcaacatac
    14281 gccgtaaaat gtcggatgcg ctctcgccga tatgcttagt gtggctggca atatagctgt
    14341 agagttcatc atcaacttca atcgttttca tcttaatcca gtgcagtgtc ttagctgaat
    14401 acaagtcgcc aggagtatat gaataaatcc cggcagcgga tagcgtcaag cctggcttgc
    14461 agaaaaactc ggaataaacc ttatttgttg cagccctgtg gcacaatggt caacataata
    14521 ccctaacccg acaacgcgaa aaaaagaact ttgccatgaa attgaatatc cgcgcgcaaa
    14581 ctgcacaaaa ccagcacaat aattctccca tcgttcttgt ccatggtctg tttggtagcc
    14641 tcgacaacct tggcgtactg gctcgcgatc tggtaaacga tcacaatatc atccaggttg
    14701 atatgcgtaa ccacggtctt tcaccgagag atccggtaat gaattacccg gcgatggcgc
    14761 aggatcttgt tgatactctg gatgcacagc agatcgacaa agcaacattt atcggtcact
    14821 ccatgggcgg taaagcggta atggcactta ctgcactggc ccccgatcgc atcgataaac
    14881 tggtggcgat cgatatcgca ccggtcgact atcacgtacg ccgtcatgat gagattttcg
    14941 cggctatcaa cgcggtcagt gaatcggacg cacaaactcg ccagcaagca gcagcaataa
    15001 tgcgccagca tcttaatgaa gaaggggtga ttcagtttct gttgaaatct tttgttgacg
    15061 gggagtggcg ctttaacgtg ccggtattgt gggatcagta tccgcatatt gtgggttggg
    15121 agaaaatccc ggcatgggat caccctgccc tgtttatccc tggcggcaat tctccgtatg
    15181 ttagcgagca gtaccgtgat gatttactgg cacaatttcc acaggcacgg gcgcatgtga
    15241 ttgcaggcgc gggtcactgg gtccatgctg aaaaaccgga tgcggtatta cgcgctatcc
    15301 gtcgctatct caatgattaa cgcaactgat taaaaaccca gcgtccgcgt cgctttccgc
    15361 gcgtgggcgt tggcgtgact catcgcgctg atgtattatg gcgcgctatc catccgggca
    15421 gaggcctggc tgattgtttc ccccgaagtc accaagatca tggccaaaga acaaacggac
    15481 cgtacgacat tagatctgtt cgcgcacgag cgtcgaccgg gacgaccgaa aactaatccg
    15541 ctttcgcgcg atgaacagct gcgtattaat aaacgcaacc agctaaaacg cgacaaagta
    15601 cgtggcctta agcgtgtcga actgaagctg aacgcggaag ctgtcgaggc gctgaacgag
    15661 ctggcggagt cgcgcaatat gagccgtagc gaactgatcg aagagatgtt aatgcagcaa
    15721 ctggcggcgc tgcgtagtca gggcatcgtt taaatttcca ctttcatgta gcacagtgtg
    15781 cagtcctgct cgtttgctga ttccgcaaga ctgcctgttc tgctatgatt gcctttatcc
    15841 gtgggcaatt ttccaccccc atttcaataa gtttcaagag gttatttcac tcatggctat
    15901 cactggcatc tttttcggca gcgacaccgg taataccgaa aatatcgcaa aaatgattca
    15961 aaaacagctt ggtaaagacg ttgccgatgt ccatgacatt gcaaaaagca gcaaagaaga
    16021 tctggaagct tatgacattc tgctgctggg catcccaacc tggtattacg gcgaagcgca
    16081 gtgtgactgg gatgacttct tcccgactct cgaagagatt gatttcaacg gcaaactggt
    16141 tgcgctgttt ggttgtggtg accaggaaga ttacgccgaa tatttctgcg acgcattggg
    16201 caccatccgc gacatcattg aaccgcgcgg tgcaaccatc gttggtcact ggccaactgc
    16261 gggctatcat ttcgaagcat caaaaggtct ggcagatgac gaccactttg tcggtctggc
    16321 tatcgacgaa gaccgtcagc cggaactgac cgctgaacgt gtagaaaaat gggttaaaca
    16381 gatttctgaa gagttgcatc tcgacgaaat tcttaatgcc tgatgtgatg cggcgtagac
    16441 tcatgtctac gccgtattaa tagataatgc caatcaaaat aattgctaca aatttgtaac
    16501 ttttgctgtt gtacctgtac aatgtcccgg tgttcaagtg gccttgccgt tgtaaatgta
    16561 agctgtgcca cgtttttatt aacaatattt gccagggact tgtggttttc atttaggcgt
    16621 ggcaattcta taatgatacg cattatctca agagcaaatt ctgtcacttc ttctaatgaa
    16681 gtgaaccgct tagtaacagg acagattccg catgactgat aacaataccg ccctaaagaa
    16741 agctggcctg aaagtaacgc ttccacgttt aaaaatcctg gaagttcttc aggagccgga
    16801 caaccatcac gtcagtgcgg aagatttata caaacgtctg atcgatatgg gtgaagaaat
    16861 tggtctggct acggtatatc gcgtactgaa ccagtttgac gacgctggta tcgtcacccg
    16921 ccacaatttt gaaggcggta aatccgtatt tgaactgaca cagcaacatc accacgatca
    16981 cctgatttgc ctcgactgcg gcaaggttat cgaatttagt gatgattcca tcgaagcgcg
    17041 tcagcgtgaa attgccgcaa aacatggcat tcgcctgact aaccacagtc tctatcttta
    17101 cggtcactgt gccgaaggcg attgccgcga agatgagcac gcgcacgaag gcaaataagc
    17161 cagcctgaac gagaaaagcc aacctgcggg ttggcttttt tatacaaggg aaataagaaa
    17221 agcgagagtt acagctctca cttatttgtt attactgcgt atttcctgcc agaccttatc
    17281 acaatcggct ttcaccgcct gatcatttcc ggtttgcgtt gcacgcagac actgctgata
    17341 atccagtacg cgtacacttt cctggtcggc aaactgctga tgttgtttct ctttcttcag
    17401 cacgtttaac acgctctggc aggcttcaat tttttctggc gaaccttgtg cggtgttgat
    17461 acaggcgctg tatgcctctt tcagacgact gtcttcctgt ggcgcggttg attgcgcaca
    17521 agctaccagc cccgatgcca ttatggcgat gagaatcagt tttttcatag caaattcctc
    17581 aaacgtgcgg caggcacaaa acctgccgcg tcgggcatca gaagatggtg aatggtgcga
    17641 ttaccataaa tttcacgtca cgctcatcct ggaagatgtt accgtaaccg ccgccccagc
    17701 ttgggatgtc agagtggttg tcgtattcgg tgaagtgcag tttgaacatc gtgcctttgg
    17761 cgcgaccgtc ctgaatggtg tagaccgcat ccaggctgta ggcagactct tcaatagtcc
    17821 ggtttttgtc gtagtacgca tccggattgc tctgccaggt cgcaggttta gcatcccatg
    17881 cgtaaacgta ggaagcgccg atggcgaagc ctggaagatt ccagtttttc aggtcataca
    17941 tcgcaccgaa gaagaccgct ttttcgccgt tggcgttgaa gtcggaacgg ttatcccacc
    18001 agatatccag gcggccgttg gaggaagcgt aggttggagt catacgttgc aggaagtatc
    18061 cctgctgacc gtcagcctta acccaggtgc cttcgaggcg caaatccact acgtcagccg
    18121 cccggtaacc aaaggtcaac gcttgcagcc aggcggtgcc gtcataaagg tcgttgacgc
    18181 tgcgatcgtc aactttatcg cgcgtaccat agaactggta gctggtggtt aacgggctac
    18241 cggcgatatc aaatttgtag ctggctttgg caaaatattg atcgatatac ccttccgcct
    18301 gaccaaatgc cgcttccagt acgaagttat ttttgaagtc gtatttcgca ccgatggagt
    18361 gcaggtaatc aactttggtg gttttatcgt tctgataaaa ctcatccatt tccagatgcc
    18421 acggcgcttt gtattcgttg gtccacatgt aggagaaact caaggcacca gcatcgccgt
    18481 aatcaaaatt cgccccggct tccgccccct gataagtacc tggcataaag ctccagtgcg
    18541 gcgctaacag cgtttgacca gttggctgaa tgtaacctgc cctcgcccaa accggaccgt
    18601 atttaaattt ggccgcagct ttatacaggc ttataccgct tttgtcgccg gaccagtctt
    18661 cgtcataggc tttattactt tttgaaaacg cgatttcgtt cgggtgggag ctgtcgccgt
    18721 tttccgccat ttcaatcgcc gtaaacgcag caatatcaag gccgaacata tcagcagcat
    18781 aaccggactg aaaatcgagg ttggcattcc aggtggagtg agaaaggttg gttttgtatt
    18841 tgtcgccgtc ggtaacatct ttacggtcgc gttcacgctg ccagtaatat ataccgccgg
    18901 ttaaggttga atcgtcgatg aatccttcgg cgcgagcctc cggcattgcc ataaagcccg
    18961 acattgctgt aacaccggcg atagccagcg ccagcgtact acgtttgcca ctaaacgtac
    19021 gcatgggtta atcctctttg acgtataaat tgctgcacca aaggtgaaca gcgaaaaaat
    19081 aaaaattaaa aaagtagcgt tgagaaaagt ctcgctgact acaggaacag actatttttc
    19141 gcgacgcgaa ttatcaatct ctttctgtaa ccagaatgta agaacatgag caaccgcaca
    19201 tattttgatg atttttgtta caaggcttaa gtttgcggtt gaaaggcttg cagaagaata
    19261 aaagcgtgaa tttccagcaa gatgaaagcg gttacatttt atttaattat ggtttgtgat
    19321 tttaaattta ttttatggat aaattgcgaa gcggaattaa ttcgcgaaga aaagcagaaa
    19381 aaacgccgca gagtgcggcg tttgggattg tttgctattt cttgccggat gcgacgtaaa
    19441 tgcctcaccc ggcctggtag aacttaaaat tactcgccta ctttcgccca ggtatcacgc
    19501 agcccaacgg tgcggttaaa taccggtttt tccgctgtgg aatggcggct gtcgaggcag
    19561 aagtaacctt cacgctcaaa ctggaatgct ttacccgcaa ccgcatcttt cagcgacggt
    19621 tcagcaaagc cctgtttgat caccagcgat tccgggttaa tcaccgacag gaaatcatcc
    19681 gcagcaccag ggttcggcac gctgaacaga cggtcgtaca gacggatttc aaccggcagc
    19741 gcatgtgccg cgctcaccca gtgaataaca cctttgactt tacgaccatc tgccggatct
    19801 ttgcttaagg tgtcggcgtc ataagtacag aagatggtgg tgatattgcc ttcggcatct
    19861 ttctccacgc gttcagcctt aatgacgtaa gcattacgca gacgcacttc tttacccagc
    19921 accagacgtt tgtactgctt gttagcttct tcgcggaaat cggcgcgatc gatccaaatc
    19981 tcaccgctaa acggcacctg acgactcccc atttccggtt tgttcggatg gttcggcatg
    20041 gtgaccattt cgccttcgcc ctgatagttt tcgataacca gtttcaccgg atcgataacc
    20101 gccattgcgc gcggcgcatt ttcgttgaga tcttcacgga tgcaggattc cagcgatgcc
    20161 atctcaatgg tgttgtcctg cttggtcacg ccgatgcgtt tgcagaactc acgaatagaa
    20221 gcagcagtgt aaccacgacg acgcagaccg gaaatggtcg gcatacgcgg gtcatcccag
    20281 ccttcaacgt gcttgtcggt caccagcagg ttcagcttac gcttggacat cacagtgtat
    20341 tccagattca ggcgcgagaa ttcgtactgg cgcgggtgaa caggaatggt gatgttgtcc
    20401 agcacccagt cgtacaggcg acggttgtcc tggaactcaa gcgtacacag agagtgcgta
    20461 ataccttcca gggcatcgct gatgcagtgg gtgaagtcgt acatcgggta gatgcaccac
    20521 ttgttgccag tctggtggtg ttcagcaaat ttaatacggt acagcaccgg atcgcgcatc
    20581 acgataaacg gtgaagccat gtcgattttc gcacgcaggc aggctttacc ttcttcaaaa
    20641 ccaccggcac gcattttttc gaacagcgcc aggttctctt caacgctgcg gtcgcggtac
    20701 gggctgtttt tacccggttg cgtcagggtg ccgcggtatt cgcggatctg ttccggcgtc
    20761 agttcatcaa cgtacgccag gcctttattg atcagttcga tcgcataggc gtggagctga
    20821 tcaaaataat cggaggagta acggacgtta ccagaccagt gaaaacctaa ccactctacg
    20881 tcgtttttga tcgactcaac atactcgata tcttctttta ccgggttagt gtcgtcgaaa
    20941 cgcaggttgc actggccttt atagtcctgg gcgatcccga agttcaggca gatagatttc
    21001 gcatggccaa tatgcagata gccattcggc tccggcggga aacgggtgtg tactgtggtg
    21061 tgcttaccac tggccagatc ttcatcgatg atctgacgga taaagttagt cgggcgggct
    21121 tctgcctcac tcatcgtgga ttcctcaaag cgtaaacaac gtataacggc gtatgatctt
    21181 ataagcggga caggctgaca actgttagtt ttttaaaatt gataatctaa caaggattat
    21241 ggggatgatt gatgaaaaaa aaagcggcag agaagatctc caccgcttgt cattgttgga
    21301 tgcgacgctc aagcgtcgca tcaggcataa agcagattac tttttgattt catacagcgg
    21361 tgtttgaccc gccacaacat ggccctgagc tttaatgatc aagccactga aatcgtcgat
    21421 attgctgcaa accaccgggc taatcatcga gcgggcgtta gcgttcaggt aatccagatc
    21481 catttccaga atcggttgcc ctgcgcttac ctgcgcaccc tcttccacca gacgtttaaa
    21541 gcctttacct tccagcgcta cggtgtcgat acccatatgg acgacgatct ccgcgccttt
    21601 ttcggtttcc aggcagaacg cgtggttggt gttgaagatt ttcacgattg tccctgcggc
    21661 tggtgatacg acgattttat ctgtcggttt caccgccaca ccgtcaccca ccgctttgct
    21721 ggcgaatgct tcgtcaggaa cctgatccag tgccacgaca tcaccggtaa tcggcgatac
    21781 cagctccgcg atagataccg cgtttggtac agcctgcggt tttgctacag gcgcggcagt
    21841 tgccggagtt gcttcagctg acgcagcggc taccggacca cgggcaacga ctttcttcat
    21901 cgcatcgccg atggattctg ctttcgcgcc aacaatcacc tgaatagtct gtttgttcag
    21961 tttcactaca ccagaagcac ccagacgttt acacatcgta tcgttgacgc gggcagagtc
    22021 agccacggtc aggcgcagac gggtgataca ggcgtcaatt gctttcaggt tgtcagtgcc
    22081 gccaaccgca gcaatatagt tggtcgccag ttgattgagg ccttcttcag tgttgctgtt
    22141 ggcttcttca gtaacgatct cgtcttcttt atcttcacga cccggcgttt tcaggttgaa
    22201 catgcggata accaaactga acaccacgaa gtagatagcg aagaagacaa cacccataac
    22261 cagcagcatc cagacgttct ggctggcggc cggcaggtta tacatcaacg cgtagtcgat
    22321 agcccccgct gagaaagaga agccagcatg gatacccagc agtgttgcca caaacaggct
    22381 gataccggtc agcagtgcgt gcaggaggta cagcagcggt gcaaggaaca tgaacaggaa
    22441 ttccagcggc tcagtcacac cggtcaggaa cgcagtaaca gcaacagaaa gcagcatccc
    22501 gccaaccatc ggacgacgct ctttcggtgc tgcgaagtac atcgccagcg ccgcacccgg
    22561 cagaccgaac atcataatcg ggaagaagcc ggacatgaac atccccgcgg tgccgtcacc
    22621 ggcatagaag cggttgatat caccgtggaa aaccgtaccc gccgcgttgg tgaattcacc
    22681 aatctggaac caggcgatgg tgttcagcac ctgatgcaga ccggttggga tcagcagacg
    22741 gttgatgaaa ccaaagatac cggaacccag cgcgcccgca gaaacgatcc actcgcctcc
    22801 tgcatggata gcgtgctgta ccggcggcca gacgtaacca aaaatggccg ccagcaccag
    22861 gcagaagaag ccggtggcaa tcggcacaaa gcgtttgccg ccgaagaaac tcaggaagtc
    22921 cggcagttta atatcggacc aacggttata ggctgcgcca ccaaccagac cggtaatgat
    22981 acccgccagt acacccatgt taatttctgg gttgatggtc accatcgctt tggttaacac
    23041 aaagtaacct accgcacccg ccagcgccgc cgcacctgcg ctgtctttcg accagctgga
    23101 tgccacaccg atggcgaaga ttaatgcgag gttatcaaaa atcgcaccgc ccgcctgggc
    23161 aataaacgca acgttaagta aatctggctg accgaatcgc agcaacagtg ccgccaccgg
    23221 cagcaccgcg atagggagct gtaacgccct accgagtcgc tggaaaaaac ctaaaatatt
    23281 catcttattc cccctacgag aaccctattt ggctcgttta aagccgtatt tttattttgc
    23341 tgcaaattgt actgccgatg ttctgtaatc agattgttag atcatctgct acagagtgtg
    23401 tgaaaattta attcgtatcg caaattaaac gcgtgtcttt tgtgagtttt gtcaccaaat
    23461 atcgttatca tcactccctt ttactggcta aagcagaaaa cttattttat cattcaaaaa
    23521 atcaggtcgg attgacgcct gtctgcgcaa atccaggtta cgcttaaaga tgcctaatcc
    23581 gccaacggct tacattttac ttattgaggt gaataatgag actgatcccc ctgactaccg
    23641 ctgaacaggt cggcaaatgg gctgctcgcc atatcgtcaa tcgtatcaat gcgttcaaac
    23701 cgactgccga tcgtccgttt gtactgggcc tgccgactgg cggcacgccg atgaccacct
    23761 ataaagcgtt agtcgaaatg cataaagcag gccaggtcag ctttaagcac gttgtcacct
    23821 tcaacatgga cgaatatgtc ggtctgccga aagagcatcc ggaaagctac tacagcttta
    23881 tgcaccgtaa tttcttcgat cacgttgata ttccagcaga aaacatcaac cttctcaacg
    23941 gcaatgcccc ggatatcgac gccgagtgcc gccagtatga agaaaaaatc cgttcttacg
    24001 gaaaaattca tctgtttatg ggcggtgtag gtaacgacgg tcatattgca tttaacgaac
    24061 cggcgtcttc tctggcttct cgtactcgta tcaaaaccct gactcatgac actcgcgtcg
    24121 cgaactctcg tttctttgat aacgatgtca atcaggtgcc aaaatatgcc ctgactgtcg
    24181 gtgttggtac attgctggat gccgaagaag tgatgattct ggtgctgggt agccagaaag
    24241 cactggcact gcaggctgca gttgaaggtt gcgtgaacca catgtggacc atcagctgtc
    24301 tgcaactgca tccaaaagcg atcatggtgt gcgatgaacc ttccaccatg gagctgaaag
    24361 ttaagacttt aagatatttc aatgaattag aagcagaaaa tatcaaaggt ctgtaattgt
    24421 tatccctgcc ctggctcctt gctcagggca atatttttta aaatcggggg tcagaatgta
    24481 tgcattaacc cagggccgga tctttaccgg ccacgaattt cttgatgacc acgcggttgt
    24541 tatcgctgat ggcctgatta aaagcgtctg tccggtagcg gaactgccgc cagagatcga
    24601 acaacgttca ctgaacgggg ccattctctc ccccggtttt atcgatgtgc agttaaacgg
    24661 ctgcggcggc gtacagttta acgacaccgc tgaagcggtc agcgtggaaa cgctggaaat
    24721 catgcagaaa gccaatgaga aatcaggctg tactaactat ctgccgacgc ttatcaccac
    24781 cagcgatgag ctgatgaaac agggcgtgcg cgttatgcgc gagtacctgg caaaacatcc
    24841 gaatcaggcg ttaggtctgc atctggaagg tccgtggctg aatctggtaa aaaaaggcac
    24901 ccataatccg aattttgtgc gtaagcctga tgccgcgctg gtcgatttcc tgtgtgaaaa
    24961 cgccgacgtc attaccaaag tgaccctggc accggaaatg gttcctgcgg aagtcatcag
    25021 caaactggca aatgccggga ttgtggtttc tgccggtcac tccaacgcga cgttgaaaga
    25081 agcaaaagcc ggtttccgcg cggggattac ctttgccacc catctgtaca acgcgatgcc
    25141 gtatattacc ggtcgtgaac cgggcctggc gggcgcgatc ctcgacgaag ctgacattta
    25201 ttgcggtatt attgctgatg gcctgcatgt tgattacgcc aacattcgta acgctaaacg
    25261 tctgaaaggc gacaaactgt gtctggttac cgacgccacc gcgccagcag gtgccaacat
    25321 tgaacagttc atttttgcgg gtaaaacaat atactaccgt aacggacttt gtgtggatga
    25381 gaacggtacg ttaagcggtt catccttaac catgattgaa ggcgtgcgta atctggtcga
    25441 acattgcggt atcgcactgg atgaagtgct gcgtatggcg acgctctatc cggcgcgtgc
    25501 gattggcgtt gagaaacgtc tcggcacact cgccgcaggt aaagtagcca acctgaccgc
    25561 attcacacct gattttaaaa tcaccaagac catcgttaac ggtaacgagg tcgtaactca
    25621 ataagagaaa gtatgacacc aggcggacaa gctcagatag gtaatgttga tctcgtaaaa
    25681 cagcttaaca gcgcggcggt ttatcgcctg attgaccagt acgggccaat ctcgcggatt
    25741 cagattgccg agcaaagcca gcttgccccc gccagcgtaa ccaaaattac gcgtcagctt
    25801 atcgaacgcg ggctgatcaa agaagttgat cagcaggcct ccaccggggg ccgccgcgct
    25861 atctccatcg tcaccgaaac ccgcaatttc cacgcaatcg gcgtacggct tggtcgtcac
    25921 gacgccacca tcaccctgtt tgatctcagc agcaaagtgc tggcagaaga acattacccg
    25981 ctgccggaac gtacccagca aacgctggaa catgccctgt tgaatgccat tgctcagttt
    26041 attgatagct accagcgcaa actgcgcgag ctgatcgcga tttcggtgat cctgccaggg
    26101 cttgttgacc cggacagcgg caaaattcat tacatgccgc atattcaggt agaaaactgg
    26161 gggctggtag aagcactgga agaacgtttt aaagtgacct gtttcgttgg tcacgatatc
    26221 cgtagtctgg cgctggcgga gcactacttc ggtgcaagtc aggattgcga agactccatt
    26281 ctggtgcgtg tccatcgcgg aaccggggcc gggattatct ctaacgggcg catttttatt
    26341 ggccgcaacg gcaacgtcgg tgaaattggc catattcagg tcgaaccgct gggtgaacgc
    26401 tgccactgcg gcaactttgg ctgcctggaa actatcgctg ccaacgccgc tattgaacaa
    26461 cgggtgttga atctgttaaa gcagggctac cagagccgcg tgccgctgga cgactgcacc
    26521 atcaaaacta tctgcaaagc cgcgaacaaa ggcgatagcc tggcctcgga agtgattgag
    26581 tatgtcggtc gtcatctggg caaaaccatc gccattgcta tcaacttatt taatccgcaa
    26641 aaaattgtta ttgccggtga aatcaccgaa gccgataaag tgctgctccc tgctattgaa
    26701 agctgcatta atacccaggc gctgaaggcg tttcgcacta atctgccggt ggtacgttct
    26761 gagctggatc accgctcggc aatcggcgct tttgcgctgg taaaacgcgc catgctcaac
    26821 ggtattttgc tccagcattt gctggaaaat taatgtgctt ttatagtggc gcttattgtt
    26881 gtcaatattc tgggtagtcc atgaccatta aaaatgtaat ttgcgatatc gacggcgtgc
    26941 tgatgcacga taacgtcgcc gtaccgggtg cagcggaatt tttgcacggg attatggata
    27001 aaggcctgcc gctggtgttg ctgaccaact atccttcgca gaccgggcaa gatctggcga
    27061 accgctttgc cactgccggt gtcgatgtgc cggatagcgt gttttatacc tctgcgatgg
    27121 cgactgccga tttcctgcgt cgccaggaag gcaagaaagc gtatgtggtg ggcgaaggcg
    27181 cactgattca tgaactgtac aaagccggtt tcactattac cgatgtgaac cctgattttg
    27241 tgattgttgg cgaaacgcgt tcctacaact gggacatgat gcataaagcg gcctatttcg
    27301 tcgctaacgg tgcacgcttt atcgccacca acccggacac ccacgggcgc ggtttttatc
    27361 ccgcttgtgg cgcgttgtgt gcagggattg agaaaatctc cgggcgcaaa ccgttctatg
    27421 ttggtaagcc cagcccgtgg atcatccgcg cggcattaaa caaaatgcag gcgcattcgg
    27481 aagaaacggt gattgtcggc gataacttgc gcaccgacat tctggcgggc ttccaggcgg
    27541 gtctggagac gattctggtg ctttctggtg tttcatcgct cgacgatatc gacagtatgc
    27601 cgttccgccc aagctggatt tacccgtcgg tcgctgaaat cgacgttatc tgaatagcaa
    27661 ccacgtctca gggcgtggtt tcttattttt acccatcatt aaaatcatcc cattcaataa
    27721 aactcgccaa aaattatcga tccatcaata aatacatcac cacaccctcc cctttttcac
    27781 aaatcagcaa tcaccattac gttttttatt tttgcactgc caaatcgctt gcgcacggtg
    27841 ctgctttgcg gcattttttt aaacaagcaa acacaacaag caacaaatac caggttaacg
    27901 gagaaggtta tgtgttcaat ttttggcgta ttcgatatca aaacagacgc agttgagctg
    27961 cgtaagaaag cactcgagct gtcacgcctg atgcgtcatc gtggcccgga ctggtccggt
    28021 atttatgcca gcgataacgc cattctcgcc cacgaacgtc tgtcaattgt tgacgttaac
    28081 gcgggggcgc aacctctcta caaccaacaa aaaactcatg tgctggcggt aaacggtgaa
    28141 atctacaacc accaggcact gcgcgccgaa tatggcgatc gttatcagtt ccagaccgga
    28201 tctgactgcg aagtgatcct cgcgctgtat caggaaaaag ggccggaatt tctcgacgac
    28261 ttgcagggca tgtttgcctt tgccctgtac gacagcgaaa aagatgcata tctgattggt
    28321 cgcgaccatc tggggatcat cccactgtat atgggctatg acgagcacgg tcagctgtat
    28381 gtggcctcag aaatgaaagc cctggtgcca gtttgccgca cgattaaaga gttcccggcg
    28441 gggagctatt tgtggagtca ggacggcgaa atccgttctt attatcatcg cgactggttc
    28501 gactttgatg cagtgaaaga taacgtgacc gacaaaaacg agctgcgtca ggcactggaa
    28561 gattccgtta aaagccatct gatgtctgac gttccttacg gtgtgctgct ctcgggtggt
    28621 ctggattcct caattatttc cgcgatcacc aagaaatacg ccgcccgtcg cgtggaagat
    28681 caggaacgct ctgaagcctg gtggccgcaa ttacactcct ttgctgtagg tctgccgggt
    28741 tcaccggatc tgaaagcagc ccaggaagtg gcaaaccatc tgggcacggt gcatcacgaa
    28801 attcacttca ctgtacagga aggtctggat gccatccgcg acgtgattta ccacatcgaa
    28861 acctatgatg tgacaactat tcgcgcttca acaccgatgt atttaatgtc gcgtaagatc
    28921 aaggcaatgg gcattaaaat ggtgctgtcc ggtgaaggtt ctgatgaagt gtttggcggt
    28981 tatctttact tccacaaagc accgaatgcc aaagaactgc atgaagagac ggtgcgtaaa
    29041 ctgctggccc tgcatatgta tgactgcgcg cgcgccaaca aagcgatgtc agcctggggc
    29101 gtggaagcac gcgttccgtt cctcgacaaa aaattccttg atgtggcgat gcgcattaac
    29161 ccgcaggata aaatgtgcgg taacggcaaa atggaaaaac acatcctgcg tgaatgtttt
    29221 gagtcatacc tgcccgcaag cgtggcctgg cggcagaaag agcagttctc cgatggcgtc
    29281 ggttacagtt ggatcgacac cctgaaagaa gtggcggcgc agcaggtttc tgatcagcaa
    29341 ctggaaactg cccgcttccg cttcccgtac aacacgccaa cctcaaaaga agcgtatctg
    29401 taccgggaga tctttgaaga actgttcccg cttccgagcg ccgctgagtg cgtgcctggc
    29461 ggtccttccg tcgcgtgttc ttccgctaaa gcgattgagt gggatgaggc gttcaagaaa
    29521 atggacgatc cgtctggtcg tgcggttggc gttcaccagt cggcgtataa gtaagaaatg
    29581 cgaaacagcc ccggtgaaat ccggggctgt ttcagttatt gaggtgcttg ttaattctga
    29641 tatgcagtaa ggaaatgttc cgggtttgcc acatcactgt ttgacaactc atcactgtag
    29701 gccagtattt catagattaa tttcccacca ctattgttga attccgaatt tcatcctgta
    29761 cgacaaaaga atggctgtag ggtggggaaa aaacacaaag taaacaataa ttgacgaata
    29821 tagcgccacg ctgttcgcaa cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa
    29881 agaggttgac gctgcaaggc tctatacgca taatgcgccc cgcaacgccg ataaggtatc
    29941 gcgaaaaaaa agatggctac gtagctcagt tggttagagc acatcactca taatgatggg
    30001 gtcacaggtt cgaatcccgt cgtagccacc atcttttttg cgggagtggc gaaattggta
    30061 gacgcaccag atttaggttc tggcgccgca aggtgtgcga gttcaagtct cgcctcccgc
    30121 accattcacc agaaagcgtt gtacggatgg ggtatcgcca agcggtaagg caccggtttt
    30181 tgataccggc attccctggt tcgaatccag gtaccccagc catcttcttc gagtaagcgg
    30241 ttcaccgccc ggttattggg gtatcgccaa gcggtaaggc accggttttt gataccggca
    30301 ttccctggtt cgaatccagg taccccagcc atcgaagaaa caatctggct acgtagctca
    30361 gttggttaga gcacatcact cataatgatg gggtcacagg ttcgaatccc gtcgtagcca
    30421 ccaaattctg aatgtgtcga atacgttcgg caaattcaaa aaccaatttg ttggggtatc
    30481 gccaagcggt aaggcaccgg attctgattc cggcattccg aggttcgaat cctcgtaccc
    30541 cagccaattt attcaagacg cttacatcgt aagtgcgccc agttggggta tcgccaagcg
    30601 gtaaggcacc ggattctgat tccggcattc cgaggttcga atcctcgtac cccagccaca
    30661 ttaaaaaagc tcgcttcggc gagctttttg cttttctgcg ttcattcaat gtcgaatgcg
    30721 atgttgacac gtcttatcct tcaatgtcgg atgcgacgct gccgcgtctt atccgaccta
    30781 cggttggcac gcatccggca atgttgtaag gctacaaccc taacgcatat ttcagcgcct
    30841 gacgtttcaa cacgccagca cgctctgccg ccattaaccc gagattacgc acaaaacgca
    30901 gcggcggcag attattactg aatccggcat aaaacagatc cataccactt tgcataatga
    30961 agttatccgc catgcgccgc atctggtaac gcttgaggac aggataactg gcccacgctt
    31021 caccgtagct gcgggcattc accagaacat caatcagggc atcgacatca cgataaccaa
    31081 gattcccccc ctgccccgcc agcggatgga tggtatgcgc ggcatcgccc accagcgcaa
    31141 gccccggctg cacatactgc aacgcatgtc ggcgcgtcag cggaaacgca ccagcggcaa
    31201 gcggtgtaac gtaacccaga cgcgacggga aatgcttcgc gatttccgcc tggagttgcg
    31261 ccatattcat attctgcaac tggcgaatac gtaccggaga gtcataccac accagcgatg
    31321 cccagttatc aaacaacggc agaaacgcac gcggtccgtc cggggtaaat tgctgccagg
    31381 tgctgtcgcc gggatcgttc tcacactgta cgctaatcaa catacacgac tgcgcatact
    31441 gccaggcatg aacgccaatt cccgccatct gccgcacctg cgaatttgcg ccgtcagcac
    31501 caatcaccag cttcgcgcga atcacttcac cgcccttcag ttccagctcc tgaagatcat
    31561 tatctcgatg taatgcaatc agcgagcctg gcacacgtaa cgttactttc ggatgcgctt
    31621 ccagcgcctg ccacaatgcc tgttgcagga cggtgttttc caccatatag ccaagcagcg
    31681 gcagcttaag ttcagcggcg tcaaacatca catgcgccgt ttcccactcc cacgtttcca
    31741 gtctgcggta aggatggcaa cgcatagcct gtactgcatc ccagacccct aaccctttaa
    31801 gcaatgatac cgatgcggcg ctgatcgccg agatccgcac gtccggttgg ctatcagcga
    31861 caaacggtgc aggttctgcg tgctcgatca ccgttaccga aaatccgtgc tgtgccagcc
    31921 ccagcgccag tgcgccgccg accattcctc cgccgacaat ggcaatttcc gttggttgat
    31981 ttgtcatggt caatcatcct gttagcaata tgctttaagt ttacaggatt tttaccccca
    32041 agggctgata acgctcatac tggtcacaac ggcagcaaag cattacacta tgcgccctgc
    32101 attcctggct actatttcgc aagagcaagt cgatgaccaa aaaactccat attaaaacct
    32161 ggggctgtca gatgaacgag tacgattcat cgaagatggc cgatctgctg gatgccaccc
    32221 acggctatca actgaccgac gtggcggaag aagcggatgt gctgctgctg aacacctgct
    32281 caatccgcga gaaggctcag gaaaaagtct tccatcagtt gggtcgctgg aaactgttaa
    32341 aagagaagaa tccagacctg attatcggcg tcggtggctg cgtggcatcg caagaaggcg
    32401 agcacattcg ccagcgcgcc cactatgtcg atattatttt tgggccgcaa acgctgcacc
    32461 gtctgccgga gatgatcaac tccgtgcgcg gcgaccgcag cccggttgta gatatcagct
    32521 tcccggaaat cgagaagttt gaccgtctgc cggaaccgcg cgccgaaggg ccgaccgcgt
    32581 ttgtctccat catggaaggc tgcaataaat attgcaccta ctgcgtggtg ccttacaccc
    32641 gtggtgaaga ggtaagccgt ccgtccgacg atattctgtt tgagatcgcc cagcttgcgg
    32701 ctcagggcgt gcgtgaagtc aacctgctcg gtcagaatgt gaatgcctgg cgtggtgaga
    32761 actacgacgg caccaccgga tcgtttgccg atctgttgcg tctggttgct gcgatcgacg
    32821 ggatcgatcg tattcgcttt accaccagcc atccgatcga attcaccgac gatatcatcg
    32881 aagtgtatcg cgacacgccg gagctggtga gcttcctgca tctgccggta cagagcggtt
    32941 ccgatcgcat tctgaacctg atgggccgta ctcatacggc actggagtac aaagcgatca
    33001 tccgtaaact gcgtgcggcg cgtccggata ttcagatcag ttctgacttc attgttggct
    33061 tccctggcga aaccaccgaa gacttcgaga aaacgatgaa gctgattgcc gacgtcaatt
    33121 tcgacatgag ctacagcttt atcttctctg cacgtccggg tacaccagcc gccgatatgg
    33181 ttgatgatgt tccggaagaa gagaagaagc aacgtctgta tattctgcaa gaacgtatca
    33241 atcagcaagc gatggcatgg agccgccgga tgctcggcac cacccagcgt attctggtag
    33301 aagggacttc gcgtaagagc atcatggagc tttccgggcg taccgaaaat aaccgcgtgg
    33361 tcaacttcga gggcacaccg gatatgatcg gtaaattcgt cgatgtagaa attaccgacg
    33421 tctacccgaa ctctctgcgc ggtaaagtgg tgcgtactga agatgaaatg ggtctgcgcg
    33481 tggcagaaac accggaatcg gtgattgccc gtacccgcaa agaaaacgac cttggcgtgg
    33541 gttattatca gccgtaattc tcaggcccgc cgttccggtg ggccttgttt tttctctctt
    33601 tatccccatc tttatcgcaa tgcttgcgcc tttttcctgt ggcgtgaata atttccttat
    33661 aggccggtga atgttcagct tccggcgaca tacagataag aggaacggtt tgaacataga
    33721 cactcgcgaa atcaccctgg agccagcaga caacgcgcgt ctgttgagcc tgtgcggccc
    33781 gtttgatgac aacatcaagc agctcgaacg ccgtctcggc atcgagatca atcgccgcga
    33841 caaccacttt aaactgaccg gccgtccgat ttgcgtcacc gctgcggcag acattctgcg
    33901 tagcctgtat gtcgatacag ccccgatgcg cggtcagatt caggatatcg aaccggaaca
    33961 gatccacctt gcgattaaag aagcgcgagt actggagcaa agtgcggaga gcgtgccgga
    34021 gtacggcaaa gcggtcaata tcaaaaccaa acgcggcgta attaagccgc gcacgccaaa
    34081 ccaggcgcag tacatcgcca atattctcga ccatgacatt accttcggcg ttggcccggc
    34141 gggtacgggt aaaacctacc tggcagtggc tgcggcagtt gatgccctgg agcgtcagga
    34201 gattcgccgt attctgctga ctcgtccggc ggtcgaagcc ggtgagaaac tgggcttcct
    34261 gcctggcgat ttaagccaga aagtagaccc gtatctgcgc ccgctgtacg acgcgctgtt
    34321 tgaaatgctg ggctttgaga aagttgagaa actgattgag cgcaacgtta ttgaagtcgc
    34381 gccgctggcc tatatgcgtg gtcgtacgct gaacgacgcg tttatcattc tcgatgagag
    34441 ccagaacacc accatcgaac agatgaagat gttcctgacc cgtatcggtt ttaactcaaa
    34501 agcggttatc accggcgacg tcacacagat cgacctgccg cgtaatacta aatcaggctt
    34561 acgtcacgcc atcgaagtgc tggccgatgt cgaagagatc agctttaact tcttccacag
    34621 cgaagacgtg gttcgtcacc cggtggtggc gcgtatcgtt aacgcctatg aagcctggga
    34681 agaagccgaa caaaaacgaa aagcggcgct ggcggcagaa cgcaagcgcg aagaacagga
    34741 acaaaaatga gtcaggtgat cctcgattta caactggcat gtgaagataa ttccgggtta
    34801 ccggaagaga gccagtttca aacatggctg aatgcggtga tcccgcagtt tcaggaagaa
    34861 tcggaagtga ctattcgcgt ggtcgatacc gccgaaagcc acagtctgaa tctgacctat
    34921 cgcggtaagg ataagccgac caacgtgctc tccttcccgt ttgaagtgcc gcctggcatg
    34981 gaaatgtcgc tactgggcga tctggttatc tgccgtcagg tggttgagaa ggaagctcag
    35041 gagcaaggca aaccactgga ggcgcactgg gcgcatatgg tggtgcacgg cagtctgcat
    35101 ttgttaggtt acgatcacat cgaagatgac gaagcagaag aaatggaagc cctcgaaaca
    35161 gagattatgc ttgctctggg ctatgaggat ccgtacattg ccgagaaaga ataatttgcc
    35221 aggcatttga ctggcagacg tccccgccgt tggtgattaa cgacggcgtt ttgattaact
    35281 aacatgagaa cccatacgac gccatgagcg acgacaattc acacagtagt gacacgataa
    35341 gcaacaagaa gggatttttc tccctgttac tcagccaact tttccacggt gaaccgaaaa
    35401 accgtgacga actgctggcg ctgatccgtg attccgggca gaacgacctt atcgacgaag
    35461 atacgcgcga tatgctcgaa ggggtgatgg acatcgcaga ccaacgcgtt cgcgacatca
    35521 tgatcccccg ctcccagatg attaccctga aacgcaacca gacgctggac gaatgccttg
    35581 atgtcatcat cgagtccgcc cactcacgtt tcccggtgat tagcgaagac aaagatcaca
    35641 ttgaagggat tctgatggcg aaagacttgc taccgtttat gcgcagcgat gctgaagcct
    35701 tcagcatgga caaagtgtta cgtcaggcgg ttgtcgttcc tgaaagtaag cgcgtagacc
    35761 ggatgctgaa agagtttcgc tctcagcgtt accacatggc gatcgttatt gacgaattcg
    35821 gtggggtttc cggtctggtg accattgaag acatcctgga actgattgtt ggtgagattg
    35881 aagacgagta tgacgaagaa gatgatatcg acttccgtca gctgagtcgt catacctgga
    35941 ccgtgcgcgc actggcttcc attgaagact tcaacgaagc gttcggcacc cactttagcg
    36001 atgaagaagt cgacactatc ggtggtctgg tgatgcaggc gtttgggcat cttccggcgc
    36061 gtggcgaaac tatcgacatc gacggttacc agttcaaagt ggcgatggcc gatagtcggc
    36121 gtattattca ggttcatgtc aaaatcccgg atgactcacc ccagccaaag ctggatgaat
    36181 aaaaccgaaa ctggatagat aactacatgg cttttgcctc attaattgaa cgccagcgca
    36241 ttcgcctgct gctggcgtta ttattcggtg cctgcggaac gctggccttc tctccttacg
    36301 atgtctggcc tgcggcgatt gtttcgctga tggggcttca ggcgctgacc tttaaccgcc
    36361 gcccgctcca gtctgccgct attggcttct gctggggatt tggcctcttt ggcaccggta
    36421 ttaactgggt ctatgtcagc atcgcgacct ttggcgggat gcctggcccg gttaacatct
    36481 tcctggtggt gctgctggcg gcgtatttgt cgctgtatac cggactgttt gctggcgtgc
    36541 tgtcgcgcct gtggccgaaa accacctggc tgcgcgtggc gattgccgcc cctgctctct
    36601 ggcaagtgac cgagtttctg cgcggttggg tgctgactgg cttcccgtgg ttacagttcg
    36661 gctatagcca gattgatggc ccgttaaaag ggctggcacc aataatgggc gtggaagcca
    36721 ttaacttcct gctgatgatg gttagcggcc tgctggcact ggcgttagtc aaacgcaact
    36781 ggcgtccgct ggtggtggcc gtcgtgctgt ttgcccttcc cttcccgctg cgttacatcc
    36841 aatggtttac cccgcaaccg gagaaaacca ttcaggtttc gatggttcag ggcgatattc
    36901 cgcaatcact gaaatgggac ggagaccagc ttcttaatac gctgaagatt tactacaacg
    36961 caacggcacc gctgctgggc aaatcatcgt tgattatctg gccggagtcg gcgataaccg
    37021 atctggaaat taatcagcaa ccgttcctca aagcactgga cggtgagttg cgtgataaag
    37081 gtagctcgct ggtaaccggg attgtcgacg cgcgtctcaa taagcagaac cgctacgata
    37141 cctacaacac catcatcacg ctgggtaaag gtgcaccgta cagctacgaa tcagccgatc
    37201 gctataacaa aaaccatctg gtgccgtttg gcgagtttgt cccgctggag tcgattctgc
    37261 gtccgttagc accgttcttt gatctgccga tgtcgtcgtt cagccgtggg ccatatatcc
    37321 agccgccgct gtcggtaaat ggtattgagc ttactgcggc catttgctac gagatcattc
    37381 ttggcgagca agtgcgcgat aacttccgcc cggataccga ctatctgctg actatctcca
    37441 acgatgcgtg gtttggtaag tctattggtc catggcagca cttccagatg gcgcgaatgc
    37501 gtgcgctgga gctggcgcgc ccactgttgc gcagcaccaa caacggcatt acggcggtga
    37561 ttggcccgca gggtgagatt caggcgatga tcccgcagtt cacccgcgag gtgttgacca
    37621 ctaatgtgac accgaccacc ggactcacac cgtatgcacg taccggcaac tggccgctgt
    37681 gggtgctgac ggcgctgttt ggttttgccg ctgtgctgat gagtctgcga gcacgtaaac
    37741 gttgatgtgc ctgatgtatc ctgcatgagc aaaacgtact ttctcattga gctttggggc
    37801 atggttatca tgcccctttt tcttacggtt cagggataca agccacattg ccatattggg
    37861 gatgacgaat aattgcctgc ggatcaagcg agcgcgtcct gtccatttgg atcaacccgt
    37921 tcttcgccat gacaaatatt tttctaccat ccgctgaaaa acgaaggtga ttaacgtagc
    37981 tggcaggata aacgccaata atctgcccgg cattaatatt gagcaggatg acataacctt
    38041 gcaggccata tgacaccaac gctaaatatt gaccgtccgg gctaataccc tcaacggaat
    38101 aaacgctcct gctacaccat tgcgtcataa tgctggcttg ttgatcccct acgcttatac
    38161 gtgaaatccc ctgactatcg gcaaaaataa cctcgccgtt ttcaggtcga aaacgcatcg
    38221 ccgatatcgt tccacgcgca tcacctgcgg gaaagtcttc tttctgatgc gtgatggtat
    38281 tgatccgcac gatgttacct ctgcctgctc ccggaaccag taatgttctt tcatccgggg
    38341 aaagtgccat aacccaggcg cctggtttac cgctcgtccc cgtcccgtcg tatttctcca
    38401 gcgggatatc cgccacatgc tgataagtca ccagatcaat cgctgacaca ctattactgg
    38461 tgcgattcat cacccaggct tttgtgccat cacgcgagag gataaaactg tcaggatgtg
    38521 caccggtggt aattgactga ataatctccc ctgtcgccac gttaatgacc gataacgcat
    38581 tcgcttcaaa tgacgtcgca taaatcaact tttcatccgg gcttaatgcc agcgatctga
    38641 cgccctgatc tttacagcca ccacaatgtt tgactgactt ttctttcgct atgggcgctg
    38701 taaaggtatg caggattttc ttttgtccga ggtcgaaaac aacaatgctg ttggtgtttt
    38761 caattgcggc atacaacgta ttaccatccc gactgatcgc catatcatgg atatcggcaa
    38821 tctcagcgag taaatcacca ctggcctgtt cacgacttac cggagcggat aatgtttccc
    38881 tgagggcgtt gcgttcctcc gctaactgtt gttttttttg tcgtcgctct tgttcaatct
    38941 cctgcggata aaacgcttca cgcaggcttt gcctgacctg taaaactgac tcatccatct
    39001 gctgctgcgc aagccagata gccccggtca gaagaagact gcccgcaaca ataatcccgc
    39061 cttccgtaac gagtctgttt tgcccagata aacgcgcgct cagccaaccc aacgcgccac
    39121 ccgcaatcag gccgccaatg tggcaagcgt tatctattcc tgtttgccgg gcaccgtaca
    39181 acagtgtcag cgccaccata cccaacagag gaaatactcg ccgctggttt ttatggtatg
    39241 tgcccgtacc tgaagcaaac tgtgttgcta tcgctgcacc agctattccc agaatcgccc
    39301 cggatgcccc aacgctgatg actatctgct cactttcgcg taacgcataa caggcgctga
    39361 tgagcccacc aaagacgccg gagaataacc aggtaatcag gagtcgccat ttccccagaa
    39421 tctgttccgc cacgcctccc acgaccagga atgcaaacat attcatcagc agatgagaaa
    39481 cactactgtg cagaaagata ctgctcaata agcgccaggg ttcacccgac aaagtaagag
    39541 gcgcaacgtt tgcaccgaga tggataagtg cggattgatc gacctcttca taactcatcc
    39601 attgtgcgag ataacaaaat atcccaatac agagtgcaaa aaggataagg gttattgcgg
    39661 gtaattgaac atttaacggc ttaaccgaag atgcagacat aaggtgtttc ttccttgaaa
    39721 agacaggtaa aaagcatttt tcccgtgtgc agttatcatc ccggcaaacg tcccatgccg
    39781 aaaagataat tctgtccgaa atatgacttt ttcagcctaa aattacaatc gtaattaatt
    39841 cgcccctaag tattttttgg catgtaagtt gctttattta ctgtgtaagc gatgaaatca
    39901 gccgcgacgc aaccaggttg caccaccaca aatcagcggt agcaccaaaa aggtgcggtt
    39961 taagaatttt gcttcggaat ggtgcggcgc atctcgcaaa aacaaacaat aaaacagcaa
    40021 tatctttaca tttagccaaa ctaaatgtta cctaacaggc acaacactgc acaataaagt
    40081 tgcagacgat aacaacacaa acactcacaa cgggtatcca tgcgttctta acgcagaaga
    40141 taaaggagtt ggatatgcaa ttacgtaaac ctgccacagc aatcctcgcc ctggcgcttt
    40201 ccgcaggact ggcacaggca gatgacgccg ccccggtagc gggcagtacg ctggacaaaa
    40261 tcgccaaaaa cggcgtgatt gtcgtcggtc accgtgaatc ttcagtacct ttctcttatt
    40321 acgacaatca gcaaaaagtg gtgggttact cgcaggatta ctccaacgcc attgttgaag
    40381 cagtgaaaaa gaaactcaac aaaccggact tgcaggtaaa actgattccg attacctcac
    40441 aaaaccgtat tccactgctg caaaacggca ctttcgattt tgaatgtggt tctaccacca
    40501 acaacgtcga acgccaaaaa caggcggctt tctctgatac tattttcgtg gtcggtacgc
    40561 gcctgctgac caaaaagggc ggcgatatca aagattttgc cgacctgaaa ggcaaagccg
    40621 tagtcgtcac ttccggcact acttctgaag ttttgctcaa caaactgaat gaagagcaaa
    40681 aaatgaatat gcgcatcatt agcgccaaag atcacggtga ctctttccgt accctggaaa
    40741 gcggtcgtgc cgttgccttt atgatggatg atgccctgct ggccggtgaa cgtgcgaaag
    40801 cgaagaaacc agacaactgg gatatcgtcg gcaagccgca gtctcaggaa gcctacggtt
    40861 gcatgttgcg taaagatgat ccgcagttca aaaagctgat ggatgacact atcgctcagg
    40921 tgcagacttc cggtgaagcg gaaaaatggt ttgataaatg gttcaaaaat ccaattccgc
    40981 cgaaaaacct gaacatgaat ttcgaactgt cagacgaaat gaaagcactg ttcaaagaac
    41041 cgaatgacaa agcactgaac taattacaag aaccaggggc ggaaattcca gccctctcga
    41101 ttgttacgta gcacggacag actatacgcc tgatggtcgt tccccatcgg gcctgaaaac
    41161 cgcaatacgc tgggtaacaa tcttcgaggg tagcagttaa cgctgctacc ctcttttttc
    41221 tggagtagat ttatgtctat agactggaac tggggtattt ttttacaaca agccccgttc
    41281 ggcaacacca cctatcttgg ttggatctgg agtggttttc aggtgacgat cgctttgtcg
    41341 atctgcgcct ggattatcgc tttcctcgtc ggttcatttt tcggcatttt acgtaccgtt
    41401 ccaaaccgtt ttctctccgg tctgggtacg ttgtatgtcg aactgttccg caacgtgccg
    41461 ttgattgtgc aattctttac ctggtatctg gtgatcccgg agttgctgcc agagaaaatc
    41521 ggtatgtggt ttaaggccga gctggatccc aatattcagt ttttcctttc atccatgctc
    41581 tgcctggggc tgtttaccgc cgcccgtgtt tgcgaacagg tacgcgccgc cattcagtcg
    41641 ttgccgcgtg ggcaaaaaaa tgccgcgctg gcgatgggcc tgacgctacc gcaggcttat
    41701 cgatacgtac tgctgcctaa tgcttatcgc gttatcgtcc cgccgatgac ttccgagatg
    41761 atgaacctgg tgaaaaactc cgccatcgcc tcgactattg gtctggtgga tatggcggcg
    41821 caggcgggta aattgcttga ttactcagcc cacgcctggg agtcgtttac cgctattacg
    41881 ctggcgtatg tgttgattaa cgcggttatc atgctggtaa tgacgctggt tgaacgtaaa
    41941 gttcgcctgc ctggcaacat ggggggcaaa taatgtacga gtttgactgg agttccattg
    42001 tcccttccct gccatatctg ctcgacgggc tggtgatcac gctgaaaatc accgtcacgg
    42061 cagtcgtgat tggtattttg tggggcacca tgctggcggt gatgcgcttg tccagctttg
    42121 cgcccgtagc gtggtttgcc aaagcctacg ttaacgtatt ccgctcgatt cctttagtta
    42181 tggttttgct gtggttttac ctgatcgtgc caggttttct gcaaaacgtg ctgggattat
    42241 caccaaaaaa tgatatccgt ttaatttcgg cgatggtggc gttttcaatg tttgaagcgg
    42301 cctactattc agagattatc cgtgctggta ttcaaagtat ttctcgtggt cagtcgagcg
    42361 ccgcgctggc gttgggaatg actcactggc agtcgatgaa actgattatt ctgccgcagg
    42421 cgttccgcgc gatggtgccg cttctgctca ctcagggcat cgtactgttc caggatacct
    42481 cactggtgta tgtgttatct ctggccgatt tcttccgtac cgcctcaacc attggtgagc
    42541 gtgatggtac gcaggttgag atgatcctat ttgccggatt tgtttatttc gttattagtc
    42601 ttagcgcgtc gttgttggtc agctacttga aaagaaggac agcataatga ttaccctgaa
    42661 aaatgtttca aaatggtatg gtcactttca ggtgctgacc gactgctcaa ccgaagtgaa
    42721 aaaaggcgaa gtggtggtgg tttgcggccc gtctggttcc ggcaaatcaa cgctgattaa
    42781 aaccgtcaac ggcctcgaac cagtgcagca aggtgaaatc accgtcgatg gtatcgtggt
    42841 taatgacaag aaaaccgatc tggcaaagct gcgttcccgc gtcgggatgg tgttccagca
    42901 ttttgagttg ttcccccatt tgtcgattat tgaaaacctg actctggcgc aggtgaaagt
    42961 gcttaaacgc gataaagcgc ccgctcgtga aaaagcattg aaattgctgg agcgcgtggg
    43021 actttccgca catgccaata aatttccggc gcagctttcc ggcggtcagc agcagcgtgt
    43081 ggcaatcgct cgcgcgttgt gtatggatcc tattgcgatg ctgttcgacg aaccgacatc
    43141 ggcgctggat ccggagatga tcaacgaagt gctggacgtg atggtggaac tggcgaacga
    43201 aggaatgacc atgatggtgg tgacccacga aatgggcttt gcccgcaaag tggcgaatcg
    43261 ggtgatcttt atggacgagg gtaaaattgt cgaagactcg ccgaaagacg ctttcttcga
    43321 tgatccgaaa tcggaccgcg cgaaagactt cctcgcgaaa atcctgcatt aatcactctg
    43381 gcgcgcatac ccaagtgcgc gccatctcac gctttatcgt ttcgcccctc gtatctgtta
    43441 catcctggcg ttacccttgt cgcaaagaag cacacaacaa ggagcaacaa tggcactgcc
    43501 aattctgtta gattgcgacc caggtcatga cgacgctatc gcaatagttc tcgccctcgc
    43561 ctcaccagag cttgatgtca aagcaattac gtcttccgcc ggaaaccaga caccagaaaa
    43621 aaccttacgc aatgttctgc gtatgctgac cttgcttaat cgcaccgata ttccggtagc
    43681 aggcggcgcg gtaaaaccgt taatgcgtga gttgattatc gcggacaatg tgcacggcga
    43741 aagcggtctg gatggcccgg cattaccgga accgacgttc gcaccgcaaa actgtacggc
    43801 ggtagagctg atggcgaaaa cgctgcgcga aagtgcggaa cctgtcacga ttgtgtctac
    43861 cggaccgcaa actaacgttg ccttgctgct caatagccat ccggaactgc atagcaaaat
    43921 tgcccgtatc gtgattatgg gcggcgcaat ggggcttggt aactggacgc ctgcggctga
    43981 atttaatatt tacgttgacc cggaagcggc agaaattgtc ttccagtcag ggatcccagt
    44041 ggtgatggcc ggtctggatg ttactcacaa agcacaaatc cacgttgaag acaccgagcg
    44101 tttccgcgcg attggcaacc ctgtttcaac cattgttgcc gaactgctgg atttcttcct
    44161 cgaatatcat aaagacgaaa aatggggctt tgtcggtgca ccgctgcatg acccatgcac
    44221 catcgcctgg ctgttgaaac ccgagttgtt taccactgtt gagcgctggg ttggcgtgga
    44281 aacacagggg aaatatagcc agggtatgac ggttgttgat tattattatc tgactggcaa
    44341 taaaccgaat gccactgtaa tggtcgatgt tgatcgtcag ggctttgttg atttactggc
    44401 tgatcgtctg aaattttacg cttaacacac gtcattgatt aagatcacca cgcgttacct
    44461 gggtggtctt aatctgttta tctctttttc tttcaagata taaatgcaat ggataatgca
    44521 gaactcgcca ttggtatcga tctcggcact accaatagtt taattgccgt ctggaaagac
    44581 ggtgccgcgc aattaattcc aaataagttc ggtgaatatt taacaccatc cataattagc
    44641 atggatgaaa ataatcatat tttagtcgga aaaccggctg tatcacggcg tacttcgcat
    44701 ccggataaaa cggcagcgtt atttaaacgt gcaatgggca gtaataccaa ctggcggtta
    44761 ggcagcgaca catttaacgc gccagaactg tcctctttgg tattacgctc attaaaagaa
    44821 gatgccgaag aatttctgca acgtccgatt aaagatgtgg tgatctccgt tccggcttat
    44881 ttcagcgatg aacaacgcaa gcatacccgt ttagcagcgg agttagccgg gttaaatgcg
    44941 gtacgcttaa ttaatgaacc cacagcagct gcgatggcgt atggcctgca tacccaacaa
    45001 aatacccgtt cgctggtgtt tgatctcggt ggcggcacgt ttgacgttac ggtgcttgag
    45061 tacgccacgc cggtgattga agttcacgcc tccgctggcg acaactttct tggtggcgaa
    45121 gattttaccc atatgctggt cgatgaggtt ttaaaacgcg cggatgtcgc caggaccacg
    45181 cttaacgaga gtgaactggg agccttgtac gcctgtgtgg aagcggcaaa atgtagcaat
    45241 caatcgccat tgcacattcg ctggcagtac caggatgaaa cgcgggagtg cgaattttac
    45301 gagaacgaac tggaagattt gtggttgccg ctgctcaatc gcttgcgagt gccgattgaa
    45361 caggcgttgc gcgatgcacg tctgaagccg agtcaaatcg acagtctggt gctggttggc
    45421 ggcgcgtcac aaatgccgct ggtgcagcga atcgccgtgc gtctgtttgg caaattaccg
    45481 tatcaaagtt acgacccgag caccattgtc gcgctgggcg cagcaatcca ggccgcctgc
    45541 cgcttacgca gtgaagatat tgaagaggtg atcctcactg atatttgccc ttactcgttg
    45601 ggcgttgaag ttaaccgcca gggcgtttcc ggcattttct cgccaattat tgaacgaaac
    45661 accacagtac ccgtgtcgcg tgtagaaact tactcgacca tgcacccgga acagaattca
    45721 attacggtta acgtctatca gggagaaaac cacaaagtta aaaataacat tctggttgaa
    45781 tccttcgatg tgccgttgaa gaaaaccggg gcttatcagt cgattgatat tcgctttagt
    45841 tatgatatca acggcttgct tgaagttgac gtgcttctgg aagatggcag cgttaagtcc
    45901 agagtcatta atcatagccc ggtaacattg agcgcgcagc agattgaaga gagtcggacg
    45961 cggttatccg cattgaaaat ttatccgcgc gatatgctca tcaaccgcac ctttaaggcc
    46021 aaactggaag agttgtgggc gcgggcgctg ggtgacgagc gagaagagat tggccgggtg
    46081 atcaccgatt ttgatgcggc gttgcagtca aacgatatgg cccgcgtcga tgaggtcagg
    46141 cggcgggcga gcgtttattt agccattgag acctcataat cacggctgcc ggatgcggcg
    46201 taaatgctgc atccggcatc ggcgcaaaaa acacatcgtc aactaaacaa catcgcgatt
    46261 ttgccgagag cataacctat aaccagcacc gttaaactga ttttgataat gctggtaatg
    46321 ctctgtggtt tttcaccctc atcatttttt tcttcatggt tttcaggtgt tggctcatcg
    46381 acatcttcat cctccggctc gagttttact ttcgtcaaca gcatccgggt gataatggcc
    46441 tctaatggat gagcatctcc gccttccagc cttgtattaa gacgggccag catctcttgt
    46501 ttcggaaacg cttcaagtac tacctgttcc gctacccgat acaaatccag taacggtgaa
    46561 tcttcttctg tctcgctacc gactctcgat ttccaccacg cccatgcttt gcgtttgaag
    46621 aataacgact caataaacga ttgacctaac ggcgataatt ttccgctatg taaaatttcc
    46681 gcccataaca gccgcgtccg ggcgctctca ccaggaacat atttcagcgg ttgaccttgc
    46741 gcatctacca tgctccgcgc ttcgataacc atcactaata acgcgaaata atccgggcaa
    46801 tgacgcttgc tccactgcaa caacaaatta tcagcttgtg tagatgggta actttcccgg
    46861 tacgcgcaga gatcggcaag caggctttcc ccctcgccgc agtacaacct ttgagcacac
    46921 aagtaatatc gggcatcttc gctttctggt tcttccattt gccactgttt ggctacagga
    46981 atgagttccg ccatacctgt ttgcaccgag ctgtaccagc gcaacagttt gcgatgtaac
    47041 tttgcatcat caggaatgaa ccacagacct tccatcgcca aaaacgcagt gacgtagtca
    47101 ggatggtaac ggcagcaacg ttccagcgcg aagtaatact caacagtctg gttctgcaac
    47161 gcaacgggat attccagaag gctaaggaaa tcgaaacaat caccagcctg aatggcctca
    47221 agaaaagcct ccctttcttc ctcatccatt ccttcgctgt cattaaacga atgccagttc
    47281 aaacgctctg ccagcaaaga agcacaattt aatgaaagat atcgcgcttc tatagcgatt
    47341 gcacacagcg gccagcgtaa ttgatcgaca tcctccatgt tccaggtatt taattgctga
    47401 ataaatttct gccaggcgga gggctgaaaa cgatcacttt ctgaatccag taatgtcctg
    47461 aatgcacgca gtatttcatg ttcagcggcg gcatcttttt cttcatcatc tgcttcctcg
    47521 acaggattta ccgccagtcg cagcgcctct tcataggcct ggcgtaatgc tttaaacccc
    47581 tgcggatcgg tttcgggatg acataacggt aagcgagcaa gataagcctg gcggataatg
    47641 tctatttgcg tcgtgctttc aatttcgagg atttgccaac atgttttcat aggtcgctca
    47701 catccttaag cgttaccggg cattcaggct ggacagttaa tggctgccag ggaagcgtgg
    47761 catagaaacg atcgcttccc gctgccagct cttcgaacat ccgactcccg gcgctatcgt
    47821 atttgtacag ggttagcgcc tcgcgtagcg aagtgttgtc ttcgcactga gaagattgcc
    47881 agtagagacg tcctaacgaa taggcggaaa aatagtgcat ccagctgtga tagtaatggt
    47941 gagctcgtac atagacgcac gattgcagcc ataaactctc ttcttccgtc agccagttgt
    48001 tgagcacacc gacccgtgac agaaacccca ttcgaacata atcccaggct ttgattcccc
    48061 cttctccaca gcacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg
    48121 agccaccagc aattaatgct ttccactctt caggggaaga gcgatgccat gtcagatata
    48181 agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc tgaattaaat
    48241 catcacgatt atttattccc caggatgatg agagatcgaa ttcttttatc ccggtataca
    48301 tttttggcga gtcatgcgcg ggtttgcaac cctgattaag tatttccatg ggcgcagaaa
    48361 gcgcaaaaag aagatattgt tcttccttat tcatttgtta ataatccctt aagtgcatta
    48421 actgactact ttttattatt gatgagtata aacttgacgc tttcaaaata aaaagaaaat
    48481 cgaagcattc acatatgaat aaaaaattaa tgtatatatt cgcaatattt atagttgcag
    48541 caattacctg tattagccac cccaagaaaa caacgttgcg tgataaagcc atggtgaatt
    48601 atgcctttga ttatttaagc tcaccgggca gtctaccgtt taccacggcg gccacggagc
    48661 tttccgcgat tcatggtcac tcaacgtcgc aatatcgcct tggagaattt tatcttcatg
    48721 gtagcgacgg taaaccactg gattatacac aggcgagata ctggtatgag caatcagcag
    48781 aacaggaaaa tccgcgcgca caaagcaaac tggggtggat ctacctcaaa ggtctggggg
    48841 tcaaacccga cacccgtaaa gcaattctct ggtataagga agcagctgaa caagggtatg
    48901 ctcatgctca atatacttta ggtttgatct acagaaatgg ctcaggtatt aatgttaacc
    48961 attatgaatc tcaaaaatgg ttaaaactgg ccgccaaaca acattacaaa aatgcggaaa
    49021 gattacttgc cgggcttccc gcacattaac gtcgactgtc aaccgaacag atggagtatt
    49081 ttccctatca aaccagcaaa gataaaaata tagaaaataa ttttaatgat gcccagacag
    49141 cctggcccac catcgtcaac gggcattggc tgcggttgcg gctccggttc aacagtttgc
    49201 ttctcggtaa aattcgccgc ctgaattaat aatcgacgaa taattaatgc ttccagtgga
    49261 caacgatctt ctgcatcaag gcggctcaca atcggcatta atgattcttc ggttggttgg
    49321 ttcgcctgca ctaattgttt ggcaactaaa tataatggcc ataagggatg ttctgtatca
    49381 cgcgtgagtg agatccgcgg cttacgtcct ttgtgcaaaa ccatctcgac cagtgaacgc
    49441 cccagagcag agagtttttc atcgctgagt atttcgtata aatgaaaacg cgtacgggcg
    49501 ctgtcgccgg ggatataaag tagcggtttt ccctgatcgt taaccagttc acgagcttca
    49561 atcatcatca ccactaacgg gtaataatcg acccgatgct gacggcacca ttgcaatatt
    49621 aaagcatccg cctgggtgga gggatactcg cgccagtagt cacaaagttc tgccagcaag
    49681 ctttcgcctt ctccacaata gacgcgttgt gcgtattcat aataacgcgc atcttcatta
    49741 tcagattcag cttcccgcca cgaccgggcg taatccagga gttcgggaat tcccaggcga
    49801 cctgcgctat accagcggag taaatttctg tgcagttttg catcatcggg gataatcact
    49861 gcgcgatgtt gcgctaaata caatgtcagc cattctggat gcgatgacca aatccgagcg
    49921 aacatttggt aaaaatcgat ggtgtcattt tgcacggcaa cgggcagatg cagaatggtc
    49981 tggaagttaa aaacattccc cttcgcaatg gcatataaaa aggattccag ttcttcttcg
    50041 tctatttccc cgacgttttc ctcttgcaac caccgcaatc tttctgctaa taacaccacg
    50101 cactcgaagg ataaatgggc agtgttcatg gcgattgtgc acagcgacca gcgtaattca
    50161 tcaatctctt ccatcgagca ataatttaat tgctgaatga atcgctgcca ggcggagggc
    50221 agaaaacgtt cgctatcaga ggcaagtaac gcacgaaagg cgagcagaat ttcatgttct
    50281 gctacctcat attcttctgg ttgccaaaca gatttagcag gcgactgcgc aatccgtagc
    50341 gcctcctcat acgcctgacg aagttgttta aaaccctgcg gatcggtttc tggatgaaag
    50401 gacggtaaca gcgccagata agcgcggcgg ataatatcga cgtcagtcgt ttcctctatt
    50461 tcgagaatct tccagcaatt tttcataggt cgctcatatc cttaagcgtt tccgggcatg
    50521 cagaatagta agccaatggt tgccaggaca aggtagggta atacgcatca tcactggcaa
    50581 ataattgata aaacatatca ttgccggaat cgtcgtattc tttacgcgct aagaattcgc
    50641 gtaataactg cagatgatca tcttcgggag actgccaata ttgccgacca aaggtataac
    50701 cggcaaagta ttgccgccag ttacgataat aacgtagagc ccgtagatga atgcgcgact
    50761 ggatccatag actttcttct tcgctcaacc agttattaag tacgcccatt cggcttaaaa
    50821 aacccatccg gacataatcc caggctttaa ttcctccctc accacagcat tcggcggtgc
    50881 tggcgacaaa ttgcgcataa gcctgacctt gctcatttag ttcagcaaga tagtcacgcc
    50941 attcacatgg cgaataacga aaccagcggt gataaaaccc tgccaaccga gcggcatgac
    51001 cgttatcgat cattcggtga attgtgcgca gcaggtcatc acgggcatca attccccagg
    51061 aggaagaaag gtcaaaaaag catgtaccta aatacatttt gggtgatgaa tgcgaaggta
    51121 aacagcattc attgcgtatt tccatcggag tggaaagtgc aaacaa
//
LOCUS       DAZFWL020000019        50640 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFWL020000019 DAZFWL020000000
VERSION     DAZFWL020000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 50640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.019"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(417..1355)
                     /gene="lrhA"
                     /locus_tag="ACHKNP_RS06185"
                     /old_locus_tag="pgaptmp_001237"
     CDS             complement(417..1355)
                     /gene="lrhA"
                     /locus_tag="ACHKNP_RS06185"
                     /old_locus_tag="pgaptmp_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HGU4703436.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            2275..3492
                     /gene="alaA"
                     /locus_tag="ACHKNP_RS06190"
                     /old_locus_tag="pgaptmp_001238"
     CDS             2275..3492
                     /gene="alaA"
                     /locus_tag="ACHKNP_RS06190"
                     /old_locus_tag="pgaptmp_001238"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HGU4703437.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            3576..4175
                     /gene="yfbR"
                     /locus_tag="ACHKNP_RS06195"
                     /old_locus_tag="pgaptmp_001239"
     CDS             3576..4175
                     /gene="yfbR"
                     /locus_tag="ACHKNP_RS06195"
                     /old_locus_tag="pgaptmp_001239"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4703438.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(4234..6066)
                     /locus_tag="ACHKNP_RS06200"
                     /old_locus_tag="pgaptmp_001240"
     CDS             complement(4234..6066)
                     /locus_tag="ACHKNP_RS06200"
                     /old_locus_tag="pgaptmp_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGU4703439.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(6153..6803)
                     /gene="hxpA"
                     /locus_tag="ACHKNP_RS06205"
                     /old_locus_tag="pgaptmp_001241"
     CDS             complement(6153..6803)
                     /gene="hxpA"
                     /locus_tag="ACHKNP_RS06205"
                     /old_locus_tag="pgaptmp_001241"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HGU4703440.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(6814..7308)
                     /locus_tag="ACHKNP_RS06210"
                     /old_locus_tag="pgaptmp_001242"
     CDS             complement(6814..7308)
                     /locus_tag="ACHKNP_RS06210"
                     /old_locus_tag="pgaptmp_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HGU4703441.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(7391..7846)
                     /gene="yfbV"
                     /locus_tag="ACHKNP_RS06215"
                     /old_locus_tag="pgaptmp_001243"
     CDS             complement(7391..7846)
                     /gene="yfbV"
                     /locus_tag="ACHKNP_RS06215"
                     /old_locus_tag="pgaptmp_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HGU4703442.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            8184..9386
                     /gene="ackA"
                     /locus_tag="ACHKNP_RS06220"
                     /old_locus_tag="pgaptmp_001244"
     CDS             8184..9386
                     /gene="ackA"
                     /locus_tag="ACHKNP_RS06220"
                     /old_locus_tag="pgaptmp_001244"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGU4703443.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            9461..11605
                     /gene="pta"
                     /locus_tag="ACHKNP_RS06225"
                     /old_locus_tag="pgaptmp_001245"
     CDS             9461..11605
                     /gene="pta"
                     /locus_tag="ACHKNP_RS06225"
                     /old_locus_tag="pgaptmp_001245"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU4703444.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            11795..13315
                     /gene="yfcC"
                     /locus_tag="ACHKNP_RS06230"
                     /old_locus_tag="pgaptmp_001246"
     CDS             11795..13315
                     /gene="yfcC"
                     /locus_tag="ACHKNP_RS06230"
                     /old_locus_tag="pgaptmp_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HGU4703445.1"
                     /translation="MSATTESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(13348..13890)
                     /gene="yfcD"
                     /locus_tag="ACHKNP_RS06235"
                     /old_locus_tag="pgaptmp_001247"
     CDS             complement(13348..13890)
                     /gene="yfcD"
                     /locus_tag="ACHKNP_RS06235"
                     /old_locus_tag="pgaptmp_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HGU4703446.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(13948..14499)
                     /gene="yfcE"
                     /locus_tag="ACHKNP_RS06240"
                     /old_locus_tag="pgaptmp_001248"
     CDS             complement(13948..14499)
                     /gene="yfcE"
                     /locus_tag="ACHKNP_RS06240"
                     /old_locus_tag="pgaptmp_001248"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HGU4703447.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(14555..15199)
                     /gene="yfcF"
                     /locus_tag="ACHKNP_RS06245"
                     /old_locus_tag="pgaptmp_001249"
     CDS             complement(14555..15199)
                     /gene="yfcF"
                     /locus_tag="ACHKNP_RS06245"
                     /old_locus_tag="pgaptmp_001249"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HGU4703448.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            15335..15982
                     /gene="yfcG"
                     /locus_tag="ACHKNP_RS06250"
                     /old_locus_tag="pgaptmp_001250"
     CDS             15335..15982
                     /gene="yfcG"
                     /locus_tag="ACHKNP_RS06250"
                     /old_locus_tag="pgaptmp_001250"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HGU4703449.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            16039..16401
                     /gene="folX"
                     /locus_tag="ACHKNP_RS06255"
                     /old_locus_tag="pgaptmp_001251"
     CDS             16039..16401
                     /gene="folX"
                     /locus_tag="ACHKNP_RS06255"
                     /old_locus_tag="pgaptmp_001251"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HGU4703450.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            16422..17315
                     /locus_tag="ACHKNP_RS06260"
                     /old_locus_tag="pgaptmp_001252"
     CDS             16422..17315
                     /locus_tag="ACHKNP_RS06260"
                     /old_locus_tag="pgaptmp_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HGU4703451.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(17363..18253)
                     /gene="rpnB"
                     /locus_tag="ACHKNP_RS06265"
                     /old_locus_tag="pgaptmp_001253"
     CDS             complement(17363..18253)
                     /gene="rpnB"
                     /locus_tag="ACHKNP_RS06265"
                     /old_locus_tag="pgaptmp_001253"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HGU4703452.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(18450..19223)
                     /gene="hisP"
                     /locus_tag="ACHKNP_RS06270"
                     /old_locus_tag="pgaptmp_001254"
     CDS             complement(18450..19223)
                     /gene="hisP"
                     /locus_tag="ACHKNP_RS06270"
                     /old_locus_tag="pgaptmp_001254"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HGU4703453.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(19231..19947)
                     /locus_tag="ACHKNP_RS06275"
                     /old_locus_tag="pgaptmp_001255"
     CDS             complement(19231..19947)
                     /locus_tag="ACHKNP_RS06275"
                     /old_locus_tag="pgaptmp_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4703454.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(19944..20630)
                     /gene="hisQ"
                     /locus_tag="ACHKNP_RS06280"
                     /old_locus_tag="pgaptmp_001256"
     CDS             complement(19944..20630)
                     /gene="hisQ"
                     /locus_tag="ACHKNP_RS06280"
                     /old_locus_tag="pgaptmp_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HGU4703455.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(20720..21502)
                     /gene="hisJ"
                     /locus_tag="ACHKNP_RS06285"
                     /old_locus_tag="pgaptmp_001257"
     CDS             complement(20720..21502)
                     /gene="hisJ"
                     /locus_tag="ACHKNP_RS06285"
                     /old_locus_tag="pgaptmp_001257"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HGU4703456.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(21723..22505)
                     /gene="argT"
                     /locus_tag="ACHKNP_RS06290"
                     /old_locus_tag="pgaptmp_001258"
     CDS             complement(21723..22505)
                     /gene="argT"
                     /locus_tag="ACHKNP_RS06290"
                     /old_locus_tag="pgaptmp_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HGU4703457.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(22771..23340)
                     /gene="ubiX"
                     /locus_tag="ACHKNP_RS06295"
                     /old_locus_tag="pgaptmp_001259"
     CDS             complement(22771..23340)
                     /gene="ubiX"
                     /locus_tag="ACHKNP_RS06295"
                     /old_locus_tag="pgaptmp_001259"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HGU4703458.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(23435..24952)
                     /gene="purF"
                     /locus_tag="ACHKNP_RS06300"
                     /old_locus_tag="pgaptmp_001260"
     CDS             complement(23435..24952)
                     /gene="purF"
                     /locus_tag="ACHKNP_RS06300"
                     /old_locus_tag="pgaptmp_001260"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGU4703459.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(24989..25477)
                     /gene="cvpA"
                     /locus_tag="ACHKNP_RS06305"
                     /old_locus_tag="pgaptmp_001261"
     CDS             complement(24989..25477)
                     /gene="cvpA"
                     /locus_tag="ACHKNP_RS06305"
                     /old_locus_tag="pgaptmp_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HGU4703460.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(25736..26398)
                     /gene="dedD"
                     /locus_tag="ACHKNP_RS06310"
                     /old_locus_tag="pgaptmp_001262"
     CDS             complement(25736..26398)
                     /gene="dedD"
                     /locus_tag="ACHKNP_RS06310"
                     /old_locus_tag="pgaptmp_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HGU4703461.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKSKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(26388..27656)
                     /gene="folC"
                     /locus_tag="ACHKNP_RS06315"
                     /old_locus_tag="pgaptmp_001263"
     CDS             complement(26388..27656)
                     /gene="folC"
                     /locus_tag="ACHKNP_RS06315"
                     /old_locus_tag="pgaptmp_001263"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HGU4703462.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(27726..28640)
                     /gene="accD"
                     /locus_tag="ACHKNP_RS06320"
                     /old_locus_tag="pgaptmp_001264"
     CDS             complement(27726..28640)
                     /gene="accD"
                     /locus_tag="ACHKNP_RS06320"
                     /old_locus_tag="pgaptmp_001264"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGU4703463.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(28796..29455)
                     /locus_tag="ACHKNP_RS06325"
                     /old_locus_tag="pgaptmp_001265"
     CDS             complement(28796..29455)
                     /locus_tag="ACHKNP_RS06325"
                     /old_locus_tag="pgaptmp_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4703464.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(29538..30350)
                     /gene="truA"
                     /locus_tag="ACHKNP_RS06330"
                     /old_locus_tag="pgaptmp_001266"
     CDS             complement(29538..30350)
                     /gene="truA"
                     /locus_tag="ACHKNP_RS06330"
                     /old_locus_tag="pgaptmp_001266"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGU4703465.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVMRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTRAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(30350..31363)
                     /locus_tag="ACHKNP_RS06335"
                     /old_locus_tag="pgaptmp_001267"
     CDS             complement(30350..31363)
                     /locus_tag="ACHKNP_RS06335"
                     /old_locus_tag="pgaptmp_001267"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU4703466.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(31429..32565)
                     /gene="pdxB"
                     /locus_tag="ACHKNP_RS06340"
                     /old_locus_tag="pgaptmp_001268"
     CDS             complement(31429..32565)
                     /gene="pdxB"
                     /locus_tag="ACHKNP_RS06340"
                     /old_locus_tag="pgaptmp_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HGU4703467.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            32664..33659
                     /gene="flk"
                     /locus_tag="ACHKNP_RS06345"
                     /old_locus_tag="pgaptmp_001269"
     CDS             32664..33659
                     /gene="flk"
                     /locus_tag="ACHKNP_RS06345"
                     /old_locus_tag="pgaptmp_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HGU4703468.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(33656..34834)
                     /locus_tag="ACHKNP_RS06350"
                     /old_locus_tag="pgaptmp_001270"
     CDS             complement(33656..34834)
                     /locus_tag="ACHKNP_RS06350"
                     /old_locus_tag="pgaptmp_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4703469.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(35118..36338)
                     /gene="fabB"
                     /locus_tag="ACHKNP_RS06355"
                     /old_locus_tag="pgaptmp_001271"
     CDS             complement(35118..36338)
                     /gene="fabB"
                     /locus_tag="ACHKNP_RS06355"
                     /old_locus_tag="pgaptmp_001271"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HGU4703470.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            36497..38503
                     /gene="mnmC"
                     /locus_tag="ACHKNP_RS06360"
                     /old_locus_tag="pgaptmp_001272"
     CDS             36497..38503
                     /gene="mnmC"
                     /locus_tag="ACHKNP_RS06360"
                     /old_locus_tag="pgaptmp_001272"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HGU4703471.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFNQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(38624..38902)
                     /locus_tag="ACHKNP_RS06365"
                     /old_locus_tag="pgaptmp_001273"
     CDS             complement(38624..38902)
                     /locus_tag="ACHKNP_RS06365"
                     /old_locus_tag="pgaptmp_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HGU4703472.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(38936..39484)
                     /gene="epmC"
                     /locus_tag="ACHKNP_RS06370"
                     /old_locus_tag="pgaptmp_001274"
     CDS             complement(38936..39484)
                     /gene="epmC"
                     /locus_tag="ACHKNP_RS06370"
                     /old_locus_tag="pgaptmp_001274"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HGU4703473.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(39484..40293)
                     /locus_tag="ACHKNP_RS06375"
                     /old_locus_tag="pgaptmp_001275"
     CDS             complement(39484..40293)
                     /locus_tag="ACHKNP_RS06375"
                     /old_locus_tag="pgaptmp_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGU4703474.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(40293..41117)
                     /gene="mepA"
                     /locus_tag="ACHKNP_RS06380"
                     /old_locus_tag="pgaptmp_001276"
     CDS             complement(40293..41117)
                     /gene="mepA"
                     /locus_tag="ACHKNP_RS06380"
                     /old_locus_tag="pgaptmp_001276"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HGU4703475.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSSLWKPEI
                     FSLIKLAAQDKNVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(41121..42206)
                     /gene="aroC"
                     /locus_tag="ACHKNP_RS06385"
                     /old_locus_tag="pgaptmp_001277"
     CDS             complement(41121..42206)
                     /gene="aroC"
                     /locus_tag="ACHKNP_RS06385"
                     /old_locus_tag="pgaptmp_001277"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGU4703476.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(42241..43173)
                     /gene="prmB"
                     /locus_tag="ACHKNP_RS06390"
                     /old_locus_tag="pgaptmp_001278"
     CDS             complement(42241..43173)
                     /gene="prmB"
                     /locus_tag="ACHKNP_RS06390"
                     /old_locus_tag="pgaptmp_001278"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU4703477.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            43339..43890
                     /gene="smrB"
                     /locus_tag="ACHKNP_RS06395"
                     /old_locus_tag="pgaptmp_001279"
     CDS             43339..43890
                     /gene="smrB"
                     /locus_tag="ACHKNP_RS06395"
                     /old_locus_tag="pgaptmp_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HGU4703478.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(43963..44814)
                     /locus_tag="ACHKNP_RS06400"
                     /old_locus_tag="pgaptmp_001280"
     CDS             complement(43963..44814)
                     /locus_tag="ACHKNP_RS06400"
                     /old_locus_tag="pgaptmp_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HGU4703479.1"
                     /translation="MHKIRHVICLLVLALCASGIQAATKIATLYVPAGTTSVVAKYRF
                     HLSVLTPQSVEYGTYESNSTAAASLPLVSWTGSPPGPELRMERNNTTLPDSTCPGLEE
                     YDALSPVTAWSCNELVLGVYYDGDLHGCPWIVSSYVESASTMDQRFGAGIFIGPTKVS
                     SDCPTESLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCDGSNFDER
                     GAYCRFVAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCRF
                     TYILNMY"
     gene            complement(44816..45355)
                     /locus_tag="ACHKNP_RS06405"
                     /old_locus_tag="pgaptmp_001281"
     CDS             complement(44816..45355)
                     /locus_tag="ACHKNP_RS06405"
                     /old_locus_tag="pgaptmp_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4703480.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVAGQSAHLPVVFKLKDCKGPAGYNVKVTLTGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(45352..45840)
                     /locus_tag="ACHKNP_RS06410"
                     /old_locus_tag="pgaptmp_001282"
     CDS             complement(45352..45840)
                     /locus_tag="ACHKNP_RS06410"
                     /old_locus_tag="pgaptmp_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4703481.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTNFSAYATLRVD
                     YQ"
     gene            complement(45837..46346)
                     /locus_tag="ACHKNP_RS06415"
                     /old_locus_tag="pgaptmp_001283"
     CDS             complement(45837..46346)
                     /locus_tag="ACHKNP_RS06415"
                     /old_locus_tag="pgaptmp_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4703482.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(46362..47114)
                     /locus_tag="ACHKNP_RS06420"
                     /old_locus_tag="pgaptmp_001284"
     CDS             complement(46362..47114)
                     /locus_tag="ACHKNP_RS06420"
                     /old_locus_tag="pgaptmp_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4703483.1"
                     /translation="MFNLTNTAKIVVPALALLATVVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(47134..49779)
                     /gene="yfcU"
                     /locus_tag="ACHKNP_RS06425"
                     /old_locus_tag="pgaptmp_001285"
     CDS             complement(47134..49779)
                     /gene="yfcU"
                     /locus_tag="ACHKNP_RS06425"
                     /old_locus_tag="pgaptmp_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HGU4703484.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHIEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDVGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(49861..50424)
                     /locus_tag="ACHKNP_RS06430"
                     /old_locus_tag="pgaptmp_001286"
     CDS             complement(49861..50424)
                     /locus_tag="ACHKNP_RS06430"
                     /old_locus_tag="pgaptmp_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4703485.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        12451 a        13634 c        12569 g        11986 t
ORIGIN      
        1 atttaatgcc ctttcacccc agatccttga cgactccagg ataattagat gttgttgaat
       61 cgtgtccgtt gtgaagcaat ggaaaaaata cgggtctatt ttgacaggaa tttgtgtcga
      121 ttcctccccc caaaagagag tattttcttg atctgtgaca cgcttttgtc attccataac
      181 aaaaacgcag caacaaattt acgtattttt taacatcatt gtagcaggtg atttttttca
      241 ggcgattatt tgtgcgttcg ggacgtgaat ctctggtgat tgaaaaatga acagttttgt
      301 acgttctgca ctatgcgagg aaggattttt actaaaaaaa agccgctggg gtttaaaaca
      361 cccccagcgg ctcgtttttt acactattgt ctcaggaatt atctatcgtc cgtcgattac
      421 tcgatatccc tttcaatcaa caatgaatca tccccttccg gagcagacat tgggctgtat
      481 tgccacggat tatggtagct ctccatcgcc tgatagatca cctgggccag ctcattatta
      541 ctggacgggt catagcacag caaatattcg gtatcaggta gcggcggtaa gccatctact
      601 ccactcagca cacgcaggtc cgggctcatc atctcaaccg gccttgccgt cacgccaaga
      661 ccggctttca ctgccgcacg aacggccgga agcgtcgagg cgacataagc cagtcgccat
      721 ggaatatctg ctttattaag cgtcgccagc accatatcgc gaaacggact tggatcatcc
      781 agcaatacaa gagggatcgg ctcgcctttt tgcaatacgt attccgctgc gcagtaccag
      841 tgtgttggcg aggttcgcag gttcaatgcc ttaaacgctg aaggacgatg ggtggttacc
      901 atcaaatcca cttcctggga ttccagcatt tctgccatat aggcattacg tttaacgcgg
      961 acatccagcg ctaatttcgg ataaaccgaa ctcacgcgat ttaacaggaa aggtaagatc
     1021 gtatcggcag attcatctga agcaccgata gttaacacgc cctgaagatt actgaacatt
     1081 aatgatgagc aggcctcatc attaaaacgc aggattttcc tggcgtaacc aagaagttga
     1141 atgccatgtt cagttaacag tttgttgcga ccgtgacgag cgaacagttc tttcccaacg
     1201 agttgttcca gacgctgcat ttgctgactt acggcggact gagtacgaca cacagcggca
     1261 gctgcggcag caaaagtgtt cagatcggca acagcaacaa atgttctcag cagatcgagg
     1321 tcgaggttaa ttatcggacg atttgcactt atcatatatt atcacttact ggcggctcat
     1381 actgagctgg ttaatgctgt gcacacacaa acaagcaatt ccatttgtaa tgtgcctccc
     1441 tggcagtttc atccggaacc cggacgaaag taaaaatgca tatgagttgc actaaaaaag
     1501 cgactcacat tgttccgtta taatgcctga agtagatcac agaatatatc ttcagggatc
     1561 gcatatctat taagttactc actcttttct atttatgaca tgcgcgtgtt tgtataaatg
     1621 taaatgtgag tccttgttcc actctcgtgc agcatcgctg gtcatacgcg aacacgtaca
     1681 aacagcaatg gtgaggcggc atcaagagca gggatccgtc atttatgcga gcattttacc
     1741 ccaaaaacct tttatttata agggtcattg cgaattatct gatgcaaagt tatgttatgt
     1801 taggcaaagt aatcttctat tattaataag cacatcaaaa cctttttgaa tattaaataa
     1861 taattaatta gcatcatcct cattcattaa ttccgcttaa caatagtttc acaccttgcc
     1921 cccattacga cacctcggaa atcatcacgc tgttaaactc tccgtaatgc ggaaatatca
     1981 tcatcgcgtt tcattagtga attcttaaat gaggcatttt cacacaatta tcttacagat
     2041 aaaaaaacca gacttacaat taagaatcag aacaattcac tatataacat tgcatgtaaa
     2101 gcatatacac ctcattattt tgtcattatt aagttattaa cagcacaatc gagccttccc
     2161 ctctggcaaa atcttattct gcagaccttc aaaacaccgt cctgggggag tacattgttc
     2221 taagctgact tccacggcag ggagtggcga taacagcaaa aaaggtcaag attcatgtcc
     2281 cccattgaaa aatccagcaa attagagaat gtctgttatg acatccgtgg tccggtgctg
     2341 aaagaagcaa aacgcctgga agaagaaggt aacaaggtac tgaaactgaa catcggcaac
     2401 ccagccccgt tcggttttga cgcgccagat gaaatcctcg ttgacgtgat acgcaacctg
     2461 cctaccgctc aagggtattg cgattccaaa ggtctttact ccgcgcgtaa agccatcatg
     2521 cagcactacc aggctcgtgg catgcgtgat gttaccgtgg aagatattta catcggcaat
     2581 ggtgtatcgg agcttatcgt tcaggcaatg caggcattgc tgaacagcgg ggacgaaatg
     2641 ttggttcctg caccagatta cccactatgg accgcggcgg tttcgctttc cagcggtaaa
     2701 gcggtgcatt atctttgcga tgaatcctct gactggttcc cggacctcga tgatattcgc
     2761 gctaaaatta cgcctcgtac gcgtgggatc gttattatca acccaaataa cccaaccggc
     2821 gcggtatatt ccaaagagct tttaatggag attgtggaga ttgcacgtca gcataatctc
     2881 attatcttcg ccgatgaaat ttatgacaaa attctttacg acgacgctga gcatcactca
     2941 attgcgccgc tggcacctga cctgctgacc attaccttta acggactgtc gaaaacgtac
     3001 cgcgttgcag gcttccgtca ggggtggatg gtgttgaacg ggccgaaaaa acacgccaaa
     3061 ggctacatcg aaggtctgga aatgctggct tcaatgcgcc tgtgtgctaa cgttcctgcg
     3121 caacacgcca ttcagaccgc gctgggtggt tatcagagca tcagtgaatt tattacccct
     3181 ggcggtcgtc tttatgagca gcgtaaccgc gcgtgggaac tgatcaacga tattccgggc
     3241 gtttcctgcg tgaaacctcg tggtgcgctg tatatgttcc cgaaaatcga cgccaaacgc
     3301 tttaacattc acgacgatca gaaaatggtg ctggatttcc tgttgcagga aaaagttctg
     3361 ttggtgcaag ggacggcatt caactggccg tggccggatc acttccgcat tgtcacgcta
     3421 ccgcgtgtcg atgatatcga gctgtctttg agcaagttcg cgcgtttcct ttctggttat
     3481 catcagctgt aatcttaatt tcactgccgg agattgcatc cggcagcgtt atcccgccac
     3541 aatgacctga tgatgtcatc atacgtaagg tcactatgaa acagagccat ttttttgccc
     3601 atctctcccg cctgaaactc attaaccgct ggccgctcat gcgcaacgtg cggacggaaa
     3661 atgtgtccga acacagtttg caggtagcga tggtcgccca tgcgctggca gctatcaaaa
     3721 atcggaaatt tggcggtaat gtcaacgccg aacgtatcgc tttactggcg atgtaccacg
     3781 atgcctcaga agtgctcacc ggcgatctcc ctactccggt gaaatacttc aattcgcaaa
     3841 tcgctcagga gtacaaggct attgaaaaaa tcgctcagca aaaactggtc gatatggttc
     3901 cggaagagct gcgggatatc tttgcgccgt taattgacga gcatgcatat agcgatgaag
     3961 aaaaatcgct ggtgaaacag gcagatgcac tgtgtgcata tctgaaatgt ctggaagaac
     4021 tcgcggccgg aaataatgaa ttcttgctgg caaaaacgcg actggaagcg acgcttgaag
     4081 cgcgtcgcag ccaggagatg gactacttca tggaagtatt tgttcccagc ttccatcttt
     4141 cgctcgatga gattagccag gattcaccgc tgtaagcagc cggagtctgc gtcgcatcag
     4201 gcaataagcg ccggatgcga catcaggctc ttgtcaaaac ggaaacagca tcgggatcat
     4261 caccacacaa accgccatca cgataatggt gaacggtacc cccaacttca caaagtcact
     4321 gaagctgtaa tttcccggac ctaaaaccag tgtgttaaca ggtgaagaaa ccggtgtcat
     4381 aaaggcggcg gatgctgcca tcgccacgac catcgcgaat ggataaggcg acacccccat
     4441 cgttttggca gcagccagcg caatcggagc catcaacacc gccgtcgcgg tattagagat
     4501 aaatagccca ataaccgccg acaagacaaa caaacacccc agcatcatat gtggcccgta
     4561 accaccgcca atgtccatca gccctttcac cgccagcgcg acacctcccg ttttctgtaa
     4621 tgccacagca aacggcatca tcccaacgat caaaataatg ctcggccagt gaatggattt
     4681 ataggcgctt tcagcatcta tacagcggaa tttccccatc agcaggcagg cgatgatagc
     4741 ggcaacagga ttaggaattt catctgtcag cattaacgcc accattagca ccagacagaa
     4801 aatggcatgg ggtgcctggc tgtgcgcggg cgatgcttca ctcacctctt ccggtaagtt
     4861 cagcgctacg aagtcgcggc cctgtttggc cagcataccg atcagtttcc agttacccac
     4921 aaccaggatg atatcgccca gcagcagagg ctcatccgcc agcgaacctt ccagcgccac
     4981 gccattgcgc tttagcccca ccacattcag tccgtagcgg gtacgaaaac caatttcgcg
     5041 caccgattta ccaatcagtt ctgactcagg aattaatgaa atctctgcca tgcccacatc
     5101 aagggcctgg tcagaaaaat actcgccgcg cagtaccatc ggctccagca attgctcact
     5161 acaaaattgc cggagatcga catcagccgc agacatatca ataagcaaaa cgtcacgcgc
     5221 gcgaaattca gaaaccccat taacgttcac gataacgcga cgaaaacgcc gccagcgttc
     5281 aacaccgatg acgttagcgc cataacgctc acgtaatttg agatcatcca gccgttgacc
     5341 tatcattggc gatccggggc gaatagccag acgtcgcgca cgcccggtca gtcgatattc
     5401 acggataaga tcgcgaaagg ttcgacgcgt ccagccttcg cgctgcgggg tctgggtatc
     5461 ccctttcagc atgaaacgca tcactaacat atacaaaata cccagcacca gcacgaccag
     5521 gccaataggt gttacgctaa agaaactgaa gccgtgatag ccttcacgca gcaattcact
     5581 gttgactacc aggttcggcg gcgtcgccac cagcgtcatc atgccgctaa tcagcccggc
     5641 aaaacttaac ggcatcatca gacgtgacgg cgaagtttgc atacgcatgg caacgcttaa
     5701 caccacggga ataaagatag cgacaacgcc ggttgaactc ataaacgcgc caagccccgc
     5761 gacggtcagc atcaacaaaa ccaacatttt gatttcactg ttgcccgcca ctttgaccag
     5821 ccatgttccc attacggtgg caacaccggt acggaccaaa ccatcgccaa taataaacaa
     5881 ggcggcaatc aggacaacgt taggatcaga aaagccggaa aatacttcgg ggaccgtcag
     5941 cgttccgctt aatgcaaacg cgacaataac aaacaaagcg accgcatcca tacgcactct
     6001 gcccgtcgca aacaagacga tggcaacggc cagtaatgaa agaacccaaa tcaattcacc
     6061 gttcacaact tatccttgtt aattgagggg gatgacttga ttctgccata aaaaagcccc
     6121 gacgagacgg ggctaaatca tgatcaggtg tttcactgaa taataacatc gccatttggc
     6181 tgtttggtca cagtaatttg ctccagactg tggaggacca aatcgacctc attcaggcgc
     6241 ggggtatctg ccggagcgtt aaccgcaatg acatggcaac ccgccgccag gccagaaagc
     6301 acgccagcgg gagcatcttc caccaccaca cactcctgcg gcgcaagccc cagcagctgc
     6361 gcacctaaca gatacgcatc aggttctggt tttccgcgct tcactcgctc agcggttaca
     6421 aacacctctg gtgcgggaag cccagctatt ttatggcgcg ctcgcgctac cggcatggag
     6481 ccagaagtca caatggccca cggaatacct gctttattca aatgattgag taaggcgatt
     6541 gcccccggaa gcgcggtaat accttcggtt tccgtggcct cgatgtgctc cagacgcgta
     6601 aactcggcgg caatatcagc ctcggatttg cccgccataa aatggcgcaa agaggtgatc
     6661 gcctgtttac cgtgaatgaa agccagcacc tcttccggcg ctaacccatg acgtctggcc
     6721 cagttgctcc acgcccgttc taccgcaggc agggaatcca ccagcgttcc atcaagatca
     6781 aacagaaaac ctttgcaccg cacgcgggcc tcctcaggcg ttgataattt gattaatttc
     6841 gttggcgctc aaatggtact gacgcgggca ggcatgccac acattaagca tgcgctgata
     6901 tttttcccac attggcgtct gggcgttaaa accgtgagtt ccggcgtcaa aatgggtata
     6961 gcgcccttcc acattaacca taaagcggac ataaccgagg taacgtgctt cagtggcggc
     7021 gtcaaagccg aggaaggtga cacgacgttc atcgatggat tgctggtcct gcaaattaga
     7081 ccaggaaaca tgcaacgcat gatacatctc cataatgtcg atgatagtgc ggcaggtttc
     7141 ttctttcagc tcgccaaact cgcgatccaa ttcacgcatc tgtaatccgt aaccacgctc
     7201 aataattgtt tgcaggcgac ggtaacgttc agcatttgcc ggatcgagca tagtcatcat
     7261 cttgtactga ttagacaaaa taagacgttg cgcgttggtc atttccattg ttgactcctg
     7321 tatcactata ctacggtgaa aaaaaagaag gctgagtatg ccttctttta tatgcgtaat
     7381 caggggtcaa ttacaaatca tcaaggaaag ttttatccag ttgtttgaag gcgcgcttaa
     7441 gcgtgtcagc taatgcctgg taatcaggct tgccttcaac gggtgccaac acctgtccag
     7501 actcctgcaa tttaccgcga acttcataaa accagttaag gattgcaggg ggtaatggcg
     7561 ttacagaacg cttgcccagc caccacaatc cctgcatggg taaacttaag gcgaacagcg
     7621 cagtggcaac tgccggccca agctgaccgc ccagggcaat ctgccagcag agagtaaata
     7681 cggcgatcgg cggcataaaa cggatcgcat aacgcgtcat cttgataacg cgattttcga
     7741 caaagaccgg ggcaaggcgt ttttccagcg gccacgtctt tgagtaatgc tgtccccggc
     7801 gaaacaagct aaaaaaatta acagaacgat tatccggcgt tgacatgctt cacctcaact
     7861 tcacatataa agattcaaaa atttgtgcaa attcacaact cagcgggaca acgttcaaaa
     7921 cattttgtct tccataccca ctatcaggta tcctttagca gcctgaaggc ctaagtagta
     7981 catattcatt gagtcgtcaa attcatatac attatgccat tggctgaaaa ttacgcaaaa
     8041 tggcatagac tcaagatatt tcttccatca tgcaaaaaaa atttgcagtg catgatgtta
     8101 atcataaatg tcggtgtcat catgcgctac gctctatggc tccctgacgt ttttttagcc
     8161 acgtatcaat tataggtact tccatgtcga gtaagttagt actggttctg aactgcggta
     8221 gttcttcact gaaatttgcc atcatcgatg cagtaaatgg tgaagagtac ctttctggtt
     8281 tagccgaatg tttccacctg cccgaagcac gtatcaaatg gaaaatggac ggcaataaac
     8341 aggaagcggc tttaggtgca ggcgccgctc acagcgaagc gctcaacttt atcgttaata
     8401 ctattctggc acaaaaacca gaactgtctg cgcagctgac tgctatcggt caccgtatcg
     8461 tacacggcgg cgaaaagtat accagctccg tagtgatcga tgagtctgtt attcagggta
     8521 tcaaagatgc agcttctttt gcaccgctgc acaacccggc tcacctgatc ggtatcgaag
     8581 aagctctgaa atctttccca cagctgaaag acaaaaacgt tgctgtattt gacaccgcgt
     8641 tccaccagac tatgccggaa gagtcttacc tctacgccct gccgtacaac ctgtacaaag
     8701 agcacggcat ccgtcgttac ggcgcgcacg gcaccagcca cttctatgta acccaggaag
     8761 cggcaaaaat gctgaacaaa ccggtagaag aactgaacat catcacctgc cacctgggca
     8821 acggtggttc cgtttctgct atccgcaacg gtaaatgcgt tgacacctct atgggcctga
     8881 ccccgctgga aggtctggtc atgggtaccc gttctggtga tatcgatccg gcgatcatct
     8941 tccacctgca cgacaccctg ggcatgagcg ttgacgcaat caacaaactg ctgaccaaag
     9001 agtctggcct gctgggtctg accgaagtga ccagcgactg ccgctatgtt gaagacaact
     9061 acgcgacgaa agaagacgcg aagcgcgcaa tggacgttta ctgccaccgc ctggcgaaat
     9121 acatcggtgc ctacactgcg ctgatggatg gtcgtctgga cgctgttgta ttcactggtg
     9181 gtatcggtga aaatgccgcg atggttcgtg aactgtctct gggcaaactg ggcgtgctgg
     9241 gctttgaagt tgatcatgaa cgcaacctgg ctgcacgttt cggcaaatct ggtttcatca
     9301 acaaagaagg tacccgtcct gcggtggtta tcccaaccaa cgaagaactg gttatcgcgc
     9361 aagacgcgag ccgcctgact gcctgatttc acaccgccag ctcagctggc ggtgctgttt
     9421 tgtaacccgc caaatcggcg gtaacgaaag aggataaacc gtgtcccgta ttattatgct
     9481 gatccctacc ggaaccagcg tcggtctgac cagcgtcagc cttggcgtga tccgtgcaat
     9541 ggaacgcaaa ggcgttcgtc tgagcgtttt caaacctatc gctcagccgc gtaccggtgg
     9601 cgatgcgccc gatcagacta cgactatcgt gcgtgcgaac tcttccacca cgacggccgc
     9661 tgaaccgctg aaaatgagct acgttgaagg tctgctttcc agcaatcaga aagatgtgct
     9721 gatggaagag atcgtcgcaa actaccacgc taacaccaaa gacgctgaag tcgttctggt
     9781 tgaaggtctg gtcccgacac gtaagcacca gtttgcccag tctctgaact acgaaatcgc
     9841 taaaacgctg aatgcggaaa tcgtcttcgt tatgtctcag ggcactgaca ccccggaaca
     9901 gctgaaagag cgtatcgaac tgacccgcaa cagcttcggc ggtgccaaaa acaccaacat
     9961 caccggcgtt atcgtcaaca aactgaacgc accggttgat gaacagggtc gtactcgccc
    10021 agatctgtcc gagattttcg acgactcttc caaagctaaa gtaaacaatg ttgatccggc
    10081 gaagctgcaa gaatccagcc cgctgccggt tctcggcgct gtgccgtgga gctttgacct
    10141 gatcgcgact cgtgcgatcg atatggctcg ccacctgaat gcgaccatca tcaacgaagg
    10201 cgacatcaat actcgccgcg ttaaatccgt cactttctgc gcacgcagca ttccgcacat
    10261 gctggaacac ttccgtgccg gttctctgct ggtgacttcc gcagaccgtc ctgacgtgct
    10321 ggtggccgct tgcctggcag ccatgaacgg cgtagaaatc ggtgccctgc tgctgactgg
    10381 cggctacgaa atggacgcgc gcatttctaa actgtgcgaa cgtgctttcg ctaccggcct
    10441 gccggtattt atggtgaaca ccaacacctg gcagacctct ctgagcctgc agagcttcaa
    10501 cctggaagtt ccggttgacg atcacgagcg tatcgagaaa gttcaggaat acgttgctaa
    10561 ctacatcaac gctgactgga tcgaatctct gactgccact tctgagcgca gccgtcgtct
    10621 gtctccgcct gcgttccgtt atcagctgac tgaacttgcg cgcaaagcgg gcaaacgtat
    10681 cgtactgccg gaaggtgacg aaccgcgtac cgttaaagca gccgctatct gtgctgaacg
    10741 tggtatcgca acttgcgtac tgctgggtaa tccggcagag atcaaccgtg ttgcagcgtc
    10801 tcagggtgta gaactgggtg cagggattga aatcgttgat ccagaagtgg ttcgcgaaag
    10861 ctatgttggt cgtctggtcg aactgcgtaa gaacaaaggc atgaccgaaa ccgttgcccg
    10921 cgaacagctg gaagacaacg tggtgctcgg tacgctgatg ctggaacagg atgaagttga
    10981 tggtctggtt tccggtgctg ttcacactac cgcaaacacc atccgtccgc cgctgcagct
    11041 gatcaaaact gcaccgggca gctccctggt atcttccgtg ttcttcatgc tgctgccgga
    11101 acaggtttac gtttacggtg actgtgcgat caacccggat ccgaccgctg aacagctggc
    11161 agaaatcgcg attcagtccg ctgattccgc tgcggccttc ggtatcgaac cgcgcgttgc
    11221 tatgctctcc tactccaccg gtacttctgg tgcaggtagc gacgtagaaa aagttcgcga
    11281 agcaactcgt ctggcgcagg aaaaacgtcc tgacctgatg atcgacggtc cgctgcagta
    11341 cgacgctgcg gtaatggctg acgttgcgaa atccaaagcg ccgaactctc cggttgcagg
    11401 tcgcgctacc gtgttcatct tcccggatct gaacaccggt aacaccacct acaaagcggt
    11461 acagcgttct gccgacctga tctccatcgg gccgatgctg cagggtatgc gcaagccggt
    11521 taacgacctg tcccgtggcg cactggttga cgatatcgtc tacaccatcg cgctgactgc
    11581 gattcagtct gcacagcagc agtaatctcg tcatcatccg cagctttgcg ctgcggatat
    11641 ctgaaccgga aataatcact atttccggtt ttttattctc ttaatctgca ttaatccttt
    11701 ctgattatct tgcttaactg cgctgcatca atgaattgcg ccatttcact ttgcatactt
    11761 accactttgt tttgtgcaag ggaatatttg cgctatgtcc gcaaccactg aatccaaacc
    11821 aacaagaaga tgggcaatgc ccgatacgtt ggtgattatc ttttttgttg ctattttaac
    11881 cagccttgcc acctgggtag ttccggtggg gatgtttgac agtcaggaag tgcagtatca
    11941 ggttgatggt caaacaaaaa cacgcaaagt cgtagatcca cactcatttc gcattctgac
    12001 taacgaagca ggcgaacctg agtatcaccg cgtacagctg ttcacgacgg gcgatgaacg
    12061 cccgggcctg atgaacttcc cgtttgaagg attaacctca ggatcgaaat acgggacagc
    12121 tgttggcatc atcatgttta tgctggtgat tggcggcgcg tttggcattg tgatgcgtac
    12181 aggaaccatt gataacggta tcctggcgct tattcgccat acccgcggga atgaaattct
    12241 ctttattcct gcgctgttta ttctgttttc acttggcggc gcggtatttg gtatgggaga
    12301 agaggccgtc gcctttgcca ttatcatcgc accgctaatg gtccggctgg gctatgacag
    12361 tattaccacc gtcctggtga cctatattgc cacgcaaatc ggttttgcca gttcgtggat
    12421 gaacccgttt tgtgtggtcg ttgctcaggg gattgccggc gttccggtgc tttctggctc
    12481 cgggttgcgc atcgtggtgt gggttatcgc cactctgatt ggcctgatct ttaccatggt
    12541 gtacgcctca cgagtgaaaa agaatcctct tctgtcacgc gtgcatgagt ccgaccgctt
    12601 ctttcgtgaa aagcaggctg atgttgaaca acgtccgttt acctttggtg actggctggt
    12661 attgattgtc ctgaccgccg taatggtctg ggtgatttgg ggcgtgatcg ttaatgcctg
    12721 gtttattcca gaaattgcca gccagttctt caccatgggt ctggtgattg gcatcatcgg
    12781 cgttgttttc cgccttaacg gcatgacggt taataccatg gcttcatcct ttaccgaagg
    12841 ggcgcgaatg atgatcgccc ctgccctgct ggtgggtttc gccaaaggga ttttgctgct
    12901 ggtcggtaat ggtgaagcag gtgatgccag cgtgttaaat accatcctca acagcattgc
    12961 caatgccatt agcggtctgg acaacgctgt cgcggcctgg tttatgttgc tcttccaggc
    13021 ggtatttaat ttctttgtga catccggttc tggtcaggcg gcgttaacca tgccgttact
    13081 ggcaccgctt ggcgatctgg tcggtgttaa ccgtcaggtt accgtgctgg cattccagtt
    13141 tggtgatggc ttcagccaca tcatttaccc aacctcagct tcgttaatgg cgacgctcgg
    13201 tgtttgccgg gtggacttcc gtaactggct gaaggtgggc gcgacactgc ttggactgct
    13261 gtttattatg tccagcgtcg tggtgatcgg cgctcagttg atgggctacc actaaaaatg
    13321 ttaagagccg caattgcggc tcttttttca ttctgccgtt tcagtctcta cggcttcatt
    13381 tttggcattg cgcttcatcc acaacgccag cgctttcagc gagtctggag tgaactcatc
    13441 gcagcgtgcg gtgatttctt ccggcgtcag ccagcaaact tcactgactt catcttcctg
    13501 tagggcgaag ggaccgtgag agacgcagct gaacaatgcg ccccagacac ggcaattttt
    13561 atcttcgaaa tagaactgcc cgtgctcagc aaaggggaca ccggcaatgc ccaactcttc
    13621 ttccgcttcg cgacgcgcgg attccagcag ttgctcatcg gcctggacca caccgcctgc
    13681 ggtcgcatct aacatgccgg gtaaaaagtc ttttgtctcg gtacgacgct ggaccagaat
    13741 tttgcccatg ccatcatgca cgacgatgta agttgcacga tgacgcagac actgtgcccg
    13801 catttgttcc cggctggctt gtgcaatgac ttcgttctct tcattgacaa tatccaccca
    13861 ttcagtactt gccaaacgac gctgttccat tatcaggaaa ccttcttttt ctggcgctct
    13921 tacggcgcat tttgagttgt gggtaaatta cggattaatc gcgacctgcg caatgatact
    13981 ttgatcattg agtgcgatta cactaagtac gtcattatcc agcatgccat aactcgccgg
    14041 attaccgcct ttcggaatac tcaccgagcc ggggttgaag tggaaaatct ccccccgctg
    14101 ttctgccact ggcagatggg tatggccgta caccagcaca tcgttctggt ttaaagcagg
    14161 cagattttcc gggccaaaaa gatgaccatg cgtcaaaaac agacgttgtt tttccagtaa
    14221 tacctgttgc cacggcgcgg ttatcgggaa atgcagcagc atttgatcca cttcgctgtc
    14281 gcagttgccg cgcacagcga taaccttatg tgccacttca ttaagccgtt cagcgacttt
    14341 ggctggcgcg taaccctccg gtaaagcatt acgcgggccg tgattcaaca cgtcgccaag
    14401 gatcaccagc cactgggcac cgctttgggc aaacaactcc agaacacgtt ccgtcgccgg
    14461 taacgaccca tgaatgtccg atgcaaacat cagtttcatc actcactcct cgtcgaaaaa
    14521 acgtaagcta tgatactgga ttctgtcgtt gctatcagcc agattgcttc gccgaaagtg
    14581 caataaaacg ctggacagac gctcgctgcc actggaatgt cgcataatcc accaggcgtt
    14641 ctggcacctc atcgccatgt agtaccaggc ggttaatcat tagcgccaga tcagtatcag
    14701 caatgcacca ttcaccaaat aaattcggct gacccagtac taacaaatgt tctgccatcg
    14761 caaacagttt ctctgcactg gcttttccct cggccgttag tggcgctttt ttcgcccccg
    14821 caaagacaac atccgtcgga cgctcttcgc ggatgggcat cagatcgctg cgcagccagg
    14881 cctgaatctg tcgtgcacgc gcacgatttt ctaaatcaag cggataaata cgttcccagg
    14941 tcggtggcgc aaatcgatct tccagatact ccgcaatggc agaagattca ctcaactcaa
    15001 aatcatcgat ttgtaataac ggcacacggc gtgtctgacc gtaaccttgc cacgtcggct
    15061 gcaaatgttc accgctgtcg aggtcgatgg tcttgatatg aaacgacagg cctttttcct
    15121 gcaacgccac ccaggcggat aacacataag gggagaaaaa gtgggcatct gaccaaagcg
    15181 tgatagcggg tttactcatt atgtcctcgg caataaaggg ggctggctat caaactatag
    15241 agcctttttg tcactgtcac gtgacgaata ctcacgtaag tcgccgcgct ttctatactt
    15301 gtggtgttca gatgattaaa caaccggagc tgcaatgatc gatctctatt tcgccccgac
    15361 acccaatggt cacaaaatta cgctgtttct cgaagaagca gagcttgatt atcgcttgat
    15421 taaggtagac ctggggaaag gcggtcagtt tcgtccggaa tttttgcgca tttcgcccaa
    15481 caacaaaatt ccggcaattg ttgatcattc tcctgccgat ggcggcgaac cgttaagcct
    15541 ctttgagtct ggtgccattt tgttgtatct ggctgagaaa acaggactct ttttgagtca
    15601 tgaaacgcgt gagcgcgccg ccacattaca gtggttattc tggcaggtag gcggactggg
    15661 gccgatgctt gggcaaaatc atcattttaa tcacgcagcc ccccaaacca ttccttacgc
    15721 tattgaacgt tatcaggttg aaactcagcg tctttaccat gtactgaaca agcggctgga
    15781 aaactcgccc tggctgggag gcgagaacta cagcattgcg gatattgcct gctggccgtg
    15841 ggttaatgcc tggactcgcc agcgaattga cctcgcaatg tatccggcag tcaagaactg
    15901 gcatgagcgg atccgttcgc gccctgccac cgggcaggca ctgctaaaag cacaactcgg
    15961 tgatgagcgt tcggatagtt aacagaaaca ggttctcgtg tattatttca tcctaagtaa
    16021 aacaacggag aacctgcaat ggcacaacct gccgctatta ttcgtataaa gaaccttcgt
    16081 ttgcgtacgt ttatcggaat taaggaagaa gaaattaaca accgtcagga tattgttatc
    16141 aatgtgacga tccactaccc cgccgataaa gcgcgcacta gcgaagatat caacgatgcg
    16201 ctgaattatc gcaccgtaac gaaaaacatt attcagcatg tagagaataa ccgtttctct
    16261 ttgctggaaa aattaactca ggatgtgctc gatatcgcac gtgaacatca ctgggtgacg
    16321 tatgctgaag tggagatcga taaactgcac gcgctgcgct acgccgattc ggtatccatg
    16381 accttaagct ggcagcgtta accgccatat cgggaggctg catgaacata gtgatcaccg
    16441 gagggacggg attaattggt cgccatttga ttccgcgttt gctggagctg ggccatcaaa
    16501 tcacggtagt gacgcgtaac ccacagaaag ccagttccgt tctcggccct cgggtgacgc
    16561 tatggcaagg gctagccgat caaagcaacc tcaacggcgt tgatgcggta atcaacctgg
    16621 ccggagaacc gattgctgat aaacgctgga ctcacgagca aaaagagcgt ctctgccaaa
    16681 gccgctggaa tatcacgcaa aaactggtcg atttgattaa tgccagcgac acgccgccgt
    16741 cggtactcat ttccggctcg gcaacgggct attatggcga cttaggtgaa gtggtggtta
    16801 ccgaagagga accgccgcat aacgaattta ctcataaact ctgcgcccgc tgggaagaga
    16861 ttgcctgccg ggcgcaaagt gacaaaacgc gagtgtgcct gctgcgaacc ggcgtggtac
    16921 tggcaccgga cggcggtatt ctcggtaaaa tgctgccacc gtttcgtctt ggcctgggcg
    16981 ggccgattgg ttccggtcgg cagtatctgg cctggattca tattgatgat atggtcaacg
    17041 gcattctctg gctgctggat aacgacctgc gcgggccatt taatatggtt tcgccctacc
    17101 cggtacgcaa tgaacaattt gcccatgcgc tcggtcaagc gctgcatcgc ccggccattt
    17161 tgcgcgtccc cgcaaccgcc attcggctgt taatgggcga atcttcaata ctggtattag
    17221 gtggacaacg cgcgctgcct aaacggctgg aagaagcggg ttttgcgttt cgctggtacg
    17281 atttagaaga ggcgctggcg gatgtcgttc gctgatgtgg tttacagcaa acatccgcca
    17341 gttaactccc ggtgttacag gattagtggc tttgcgcgat aagatcgtct ggtgaaagtc
    17401 gggtcaccat cataactaac tctctgtcta aacctctatc cagcatctcc tgagcaatac
    17461 gcagggcttc ttcgtgtttg ccctgcattg cgccttcttc acgtaatctg tcagcaatgg
    17521 tcatcagttt ctccttttct tgtggtgcgc gttctgttat ctcaccaata aatgcacgga
    17581 aacgctgggc atcccctgtt tgtaatacgt aattaaacag ggcttttagc tgtctgtcat
    17641 tagtgttccc tgtaactagc agcgaaacaa tttggtcgac taatcccaac agatcgcgct
    17701 gacgaatatg tttctgaatt aactccaaca gcgccatttt gcggtgttgc ataatctcgt
    17761 catccggcac cacggtaata tccaccaacg gaaacgctga tgaatatatt ttgcgggcta
    17821 tagcaggctc ggcaaattca tcaagccagc agagtgaata aggataagga cttctgcaac
    17881 catgataaaa cagcatcggg atcaccaatg gaagctcttt atagcccgca tcaagatggt
    17941 tttgcattgc cgcaatggaa taacgcatca tgcgaaaagc cattaattct tccggcttac
    18001 tttggtgctc tatcactaca taaatataac ccactccctc ctgcgttttc acagaccaca
    18061 agaggtcgga ataatattgc cgcaggtcgt catcaataaa actgtttggt tccagtttaa
    18121 gcgtcgttaa atcacacagt ttgcgcagcg gcgcgggaag atgaatatca ataaaatccc
    18181 gcgcggtgtc tggatggcgt aaaaaagatt taaataccgc atcatgcggc gtggaagttg
    18241 tcgatattgt catggcgttc cgtcacccct caaatttaag atgacgcgac aataaccacg
    18301 attaacaccc aatgcagcag gcagttttta tctttggaca gcgaattcag aagtattttt
    18361 agcggactgc aacttttaca tttcagcgga agctgactca caaattcccc atgaaaatac
    18421 tccggcagcg ttagccaccg gagaggaaat tatttcagcg atcccttaag gaactgttgc
    18481 agacgagggc tttgcgggtt gccaaataac tgctccggcg cgccctcttc ttctattttt
    18541 ccctgatgga ggaaaatgac gtgagtagaa acatggcgag caaagcccat ttcgtgagtc
    18601 actaccacca tggttttccc ctcttctgcc agttgctgca taatacgtaa cacttcgcct
    18661 accagttcag gatcgagcgc cgaggtaggt tcatcaaaca gtaaaacttc cggttccatc
    18721 gccagcgccc gcgcgataga aacacgctgt tgctgaccgc cggaaagatg caccggatat
    18781 ttcccctgcg cacgttcgtc tatcccgact ttcgccagat acttcaccgc ccgctcgcgc
    18841 gcttcctgct tgctcaggcc caacacctga atcggcgctt ccatgacgtt ttccagcacc
    18901 gtcatatggc tccagagatt gaagtgctgg aataccatcg tcaggcgtgt gcgcagtaag
    18961 cgcagttgat ttttatcggc gactttgagt tgaccatctt tgtcgcgcac cagattgatc
    19021 gtctggccat tgaccacgat cgacccttca ctcggttttt cgaggaagtt aatgcagcgc
    19081 agaaaggtac ttttccccga tcccgacgat ccgatgatgc ttattacatc tccggcattc
    19141 gcttgcagtg agaccccttt cagcacttca tgttcgccgt agcgtttgtg caaatcgata
    19201 acgtttaatt tattctcgga catcgtattc tcagtgcgtt gaagaaggtt tcacatgctg
    19261 caaccagcgt ttttccgctc tgcgaaagag gctgatcagg acataagaga tgattaaata
    19321 gagcaccgcg gcaatgccga aggcggtaaa aggttgatac gtggcggcgt taatatcgcg
    19381 ggctattttc agcagatccg gcacagtggc cgtaaacgcc agcgcagtag agtgcagcat
    19441 caggatcact tcgttgctgt atgccggtaa cgcaatacgc agcgcagaag gcaaaataat
    19501 gcaacgatac attttaaaag tcgagaagcc ataggctctg gcggcttcaa tttccccatg
    19561 cggaaccgaa cggattgccc cagcaaaaat ctcggtagtg taagcgcagg tgttaagcgt
    19621 cagcgccagc acggtacagt tcaggccact gcggaagaaa gcgttaagga attcggttcc
    19681 cttaacaatc tcaagcgtgt acatgccgga atagaacacc agcaactgaa catacagcgg
    19741 cgtaccgcga aaaatatagg taaataacca gattggaaac tggatgtatt tattactgga
    19801 gacacgacca atcgccagaa acagcgccag gactccgcct atcactaccg acaaaataag
    19861 cagccacaga gtgatcgcca caccagtaaa gcgataaccg tcggtccaca gcagcggttt
    19921 ccagtattca tgtaagattt cgatcacagg tcagccctct tcacacccac ggagtagcgg
    19981 cgctcaagga acagcagcac accattggaa acagtggtga aaaccaggta aatcacgcca
    20041 cagacgatgg cgaaatagaa cggttcccag gtacttttgc ctgccagttg ggtggctttg
    20101 accacatctt ccaggccgag taacgaaacc agtgcggtag atttgaggat cacctgccag
    20161 ttgttgccaa tgcctggcag ggcgtaacgc atcatcgccg gaaacatgat ccgccgaaac
    20221 acttgcccac gagtaaaacc gaacgccgtc gccgcctcta tatgcccttt cggcactgcc
    20281 ataaacgcgc cacggaacgt ttcggtaaaa taagcaccgt agataaaacc gagagtaata
    20341 ataccagcga ccatcggatc gatatcaatc tgcccgacgc ccatcgcctc cgtcaccgtg
    20401 tttagtgcaa tctgcaaacc gtagaaaatc agcagcatca acaccaagtc cggcacgcct
    20461 cgaatcagcg tggtgtaccc ttcgaaaatc agccccgaaa gccgattttg cgagagctta
    20521 ccgccagcgc caattaaacc gatgattaca gcgagcacta cagaactgat agccagctcc
    20581 agcgtgacga gcgcaccctg taaaataaca cctgaaaacc catacaacat gctgcctgtc
    20641 ctgtcgtgtg tggtgaatta ccgcctcttg tctccctccg gttacccgaa gggagcgggc
    20701 tttctttcac cgatggcgat taaccgccat aaacatcaaa atcgaagtac tttttcgcta
    20761 atttctcgta agtaccgtca gcgcgcattt cggcaaaggc tttgttcagt gcctcgcgca
    20821 gttcgttatc ttctttacgc aggcccatgc cggtccctac gccaaacagt ttttcatctt
    20881 taacagacgg gccaccgaat ttgtaatctt taccgacagg ttgtttgagg aaaccttcgc
    20941 tggcagcgac ctcatcctgg aacgcggcat caatacgtcc ggcagtcagg tcagaataaa
    21001 tgttgtcctg cccctgatac gagacgattt caatgccttt tggtgcccaa tgttcattac
    21061 cgaacgtctc ctgggtggtg ccctgcaata cgcctacccg tttgcctttc agcgactcga
    21121 ctgtcggctg aatgtcggaa tttttcgcca ccaccaaacg agaatcggca gcgtacagtt
    21181 tgtcggtgaa ggctatttct tgctgacgtt tttccgtaat ggaaagcgat gacatgatgg
    21241 cgtcaatctt cttcgctttt aaggacggga ttaacgcatc cagcggattt tcgacaaacg
    21301 tacattgcgt attgatgcgt ttgcataatt cctttgccag atcgatatcg aagccaacca
    21361 gttcgccttg tgaatttttt gattcaaatg gcgcataggt cgggtcggta ccgatgcgga
    21421 tgttttgcgg aatcgcagca aacgccgcag ttgcgctgga gaaggccaga accagagaga
    21481 gcgatagcac cagttttttc ataatgtcct caactgacag tctttttata gggaatattt
    21541 acaggtttgt tacacttatc gtgccataaa attgaccatc aaggcaaaat atcccgcccc
    21601 gttagacgta ttatttttca ttaaaaatgc ttaagtatgc atttcaatgc accacgatag
    21661 tgcaacaaac ctggcgtgca ccatgatgat cattccatca ggtacagctt cccagcgacg
    21721 tatcagtcac cgtagacatt aaagtcgaaa tactttttcg ccatcttgtc gtaggtgccg
    21781 tcctgacgca gctcgccaag cgccttattg aaggcagccg tcagttcagc atcatcttta
    21841 cgtagcccta caccggtgcc atcaccgaag tattttttgt cttttactga tgagccagca
    21901 aaggcgaaat ctttaccagc aggttgcttg aggaatcctt cgctggcagc aacttcatct
    21961 tgtaacgcag catccagacg tcctgcagcc agatcggaat agaccaaatc ctggttggca
    22021 taggctacca catccacgcc tttgctacgc caggtctcgt tagcgtaagc ttcctgggtt
    22081 gatccctgca gcacaccaac atgtttacct ttcagtgaat ccagcgttgg ctgaatcggt
    22141 gaacctttgg cagcaatcaa acgagaatct gcggcgtaca gcttgtcgga gaaggcaatc
    22201 tcctgctgac gtttatcggt aatggaaagc gacgaaataa tggcgtcgat ttttttcgct
    22261 ttcagtgagg ggatcagcgc gtcaaagtca ctggcaaccc aggtacattt cacctgcatc
    22321 cgtttgcaca tctcgttacc gagatcgata tcaaagccaa caaaatcacc tttagcatct
    22381 ttcgacgaga acggcgcata ggtggtatcg gttccgatac gtaccgtctc cggtagcgcc
    22441 gcatagctgg aagccgctgc ggagagaccg actaacaaag acagagcgag aatcgacttc
    22501 ttcatacata accctcaagt gaaaatggct tttttatgtt gtgtattgtg ttgtgtgttt
    22561 gcaggctctt tcatgcaggt cttatgccat cttgacgaca acagtaacat tcaacgttaa
    22621 atatgttaat aagacgttgc attattgtcc tgaagttgaa gatagcaggt atagcggttg
    22681 aatcgcagcg tttcgattga atggcagaaa caaaatgtcg aggatttgat cgcggttgca
    22741 aaattgccct gaaacagggc aacagcggag ttatgcgccc tgccagcggg caaagagatc
    22801 ttcaggaagg gttattgcaa actggtcaag aacacgatta accgtctgat ttatcacatc
    22861 atcaagggat tgcgggcgat gataaaacgc cggaacggga ggcataatca ccgcaccgat
    22921 ttctgccgcc tgagtcatta aacgcagatg gcctaagtgc aatggtgttt cacgcacgca
    22981 gagcaccaac gggcgacgct ctttcagcac cacatctgcc gcacgggtca gtaagccatc
    23041 agtatagcta tggacaatgc cggaaagggt tttgattgaa cagggtaaaa tcaccatccc
    23101 cagcgtctgg aaagaaccgg aagagatgct ggcggaaata tcgcgcgcat cgtgcgtgac
    23161 atcagctaat gcctgcactt cgcgcaggga aaaatccgtt tcgagggata aggtctggcg
    23221 cgccgcctgg ctcatcacca gatgcgtttc gatatctgtg acatcgcgca gaacctgtaa
    23281 taagcgcacg ccataaatcg cgccgctggc accgctgatg cctacaatga gtcgtttcat
    23341 aaatagttgc cctgtcagac ttgcaggcag actttgcagt atttcggcgg gagttgcaag
    23401 tcagggtgcc agaccggcac cctcagcgaa ggcatcatcc ttcgttatgc atttcgagat
    23461 tttccacttc gttctgacgt tgcactgctt tggcgtcatc attacgtaac gtatcgagga
    23521 aatcgaggta gccctgatca acatctttgg tgacgtagac gccgttgaac accgagcatt
    23581 caaactgctg gatatccgga ttttcagcgc gaacggcgtc gatcagatcg ttcagatcct
    23641 ggaaaatcaa cccgtcagca ccgatgatct ggcgaatttc atcaacttcg cgaccgtgag
    23701 cgatcagttc cgtggcgctc ggcatatcaa taccataaac gttcgggaag cgaatttccg
    23761 gtgccgcaga agcgaggtac actttcttcg ctccggcttc gcgtgccatc tcgataatct
    23821 gctcagaagt ggtgccacgg acgatggagt cgtcgaccag caggacgttt ttatcgcgga
    23881 actcggcgcg gttggcattc agtttacggc gcacggactt acgacgcagc tgctggcccg
    23941 gcatgataaa ggtgcggcca acatagcggt ttttaacgaa gccctggcgg tacggtttgc
    24001 ccagaatacg agcaatttcc agcgcgatat cacacgaggt ttctgggatc gggatcacca
    24061 cgtcgatatc cagatcttcc cattcgcggg caattttctc gcccagtttc gtgcccatat
    24121 tcacacgcgc gctgtaaacg gaaattttgt cgatgaacga gtccgggcga gcaaagtata
    24181 catactcaaa caggcacgga ttgctgaccg gattgtcagc acattgacgg gtaaacaact
    24241 gcccttcttc agtgatgtaa atcgcttcgc ccggcgcgac gtcacgcagg aaatcaaagc
    24301 ccagcgtatc gagcgctacg ctttcggaag cgaccatata ttctgtacgg ttctcgtcaa
    24361 tatcacgttt tcccagtacc agcggacgaa tcccgtttgg atcgcggaaa gcaaccatac
    24421 cgtggccgat aatcatcgcc acacaggcat atgcgccgcg gattaagcgg tttgtggcag
    24481 caatggcagc gaaaatattg tcggcttcca gcgggtagtg gcggaagttg tccagctcgc
    24541 tagcgaagat attaagcaga atttccgagt cggaagtggt gttgatgtgg cggcgttttt
    24601 cttcaaacag ttttttacgc aactcgtgag cgttggtcag attgccgttg tgggcaagcg
    24661 taatgccata cggagagtta acgtaaaacg gctgcgcttc agaggcgctg gagctgccag
    24721 ccgtagggta acgcacatga ccaatgccca tattgccctg caaacgctgc atatggcgag
    24781 cttcaaatac atcgctcacc agcccgttcg ctttacgcaa acggaagcag ttattggcat
    24841 ctatggtgat gatgccggcg gcatcctgac cgcgatgctg aagcaccgtt aaggcatcat
    24901 aaatcgactg gttaaccggc ataacaccgg cgataccgac aataccgcac atacgtcttt
    24961 ttcctcgtta agccacatct cagagcactt acgctctggg caagaaactt gacgagcttt
    25021 gcagataatc aaaaaaccat ctgatgataa aactgaattg cgggatcagc tgtgatttgc
    25081 tccagtcttc gcttttcgac accccggtaa aggagtcgag aaagaagaga atggcggcaa
    25141 caatcaacac accgcgcaac gcaccgaaac agacgcccag cacccgatcg gtgcctgaca
    25201 accccgtttt ctccaccaac tggcctatca cgaagttcac gatagcacca acgatcaggg
    25261 tagcgataaa cagtaccgcg atggcaattc catttcgaac cagttcgtct tcaaagcccg
    25321 taaaccagac tgacaggtaa gtgtagtaat gactggcaac aaagaaagca caaccccatg
    25381 tcaccagcga taacgcttca cgaacaaagc cgcggatcag gctaaccaga gaggaaaaag
    25441 caatcaccgc gattatggcg taatcaatcc agaccatatg tgtcccacga ttttacgccc
    25501 tgtcatcctg ttcggggcgc attctaacag aaaaagaaaa cgtttgcgta gggatttcct
    25561 tcccgcgcat caataaaaat ggcgctgaaa aaatattcaa cgccatcgac tttttatgcc
    25621 tttgcggcat cgtgcaatgc gtgtcggatg cggcgtaaac gccttatccg acctacggtt
    25681 ctaacactgc ccaggcctga taagacgcgc cagcgtcgca tcaggcaaga ctgtattaat
    25741 tcggcgtata gcccattacc acgccactta agccagaaag ttgcttcaac tcacccagcg
    25801 aacctttcag cttatctttc gaggcatccg gcccaaccag aatacgggta attttaccct
    25861 gcactggcgt ggatggcgac gtataaaccc gataaccggc accgcgcagc ttaccgacaa
    25921 tctcattcac tttatcggca tttttcagcg cacccagttg cacaacatag gctttacccg
    25981 ttggcgctgc tttttcttcc acgaccggct tcggttctgg cttcggcgca ggtggtgctt
    26041 caaccttcgg tttaggcggc tccaccggtt tcgacttcgg tggggcgacc ggtgcaggtt
    26101 ccggttcaaa ctcggtgtta ttggctgcaa tagtggtcgg atcgagcgac ggtgctgcgg
    26161 catcacctgc ccgcacctct tccgctgcgc cttccggcgg ctgcgtcggc aacgcctggg
    26221 tggcggctgg catcatatca ggctcatcgc gatcgcccgc tttcggcacc agcgggatag
    26281 ccgcgaactc atcctgataa tgttttttct gcccgtccag cagccctgga agtacaatca
    26341 cccccagcgc caccagcacg atcgtgccca ctaaccgatt ctgaaactta cttgccaccg
    26401 cttctcctcg cgtcaatcac ttccatgaca tgtgcgacag tgtggaaaga accacacacc
    26461 agcacggtgt cttccgcttt agcgtccgcc attgcggcat cccatgcctg cgcaacgcta
    26521 tcaaatgatt tgccgttacc caaatgctca agcagttgtt ctgccgtggc accgcgcggc
    26581 ccttccagtg gcgcacaata ccagtcatca accacgcttt tcaaccaggc cagagttccg
    26641 gcaatatctt tatcatgtag cataccgata accgccagca cgcgcccgtt tttcggtagc
    26701 gctttcatac gcccggtgag atattccgcc gcatgtggat tatgcgcgac atcaaaaata
    26761 acgcgtggcg actcgctcac aatctggaaa cgtcccggca aaattgcgct ggcaatcccg
    26821 tcgcgaatgg cattttcact gacttccagc ccgctggcac gcagtgccgc cagcgccgtt
    26881 gcggcattcg gttgcgggac aagcggcaac ggcagatttt ccagcgtgcc gtgagcatcg
    26941 ctaaacgccc agtcatgatc ggtgacggaa tagttccact caacgccccg acgttgtaac
    27001 agtgcacctt tttcctgtgc cacatcagca atggtagaag gcatttccgg ctcaccgaca
    27061 attgccggtt tttcgctgcg gaagatgcct gctttctcgc gaccaatact ttcgcgatct
    27121 ggacccagcc agtcggtatg atccagcgca atactggtta ctaccgcgac atcggcatcg
    27181 acaatattgg ttgcgtccag acgaccgccc agccctactt ccagaatcac cacgtcaagt
    27241 tgtgcctgct tgaacagcca caacgccgac agcgtaccgt actcgaaata ggtcagggaa
    27301 atatcaccgc gtgccgattc aatctccgca aaagaggcgg tgtgggccga ttccggcaat
    27361 tcctggccct gcacacgtac gcgctcggta taacgcacca gatgcggtga actgtagacg
    27421 cccactttgt accctgccgc catcagaatc gactccagcg tacggcaggt ggtgcctttg
    27481 ccattcgtac ccgcaacggt aaacacaaat ggcgctggtt tcagaacgcc aagacgcgcc
    27541 gcgacctggc tcacgcgctc aaggccgaga tcgatagttt tactgtgcag gttttccaga
    27601 taagaaagcc acgaagccag aggcgacgcg gcttgaggag tgcgtttgat aatcatggta
    27661 tccgctgatt cgttacggtg agaatagcaa aagggcagag ccagtggccc tgcccttatc
    27721 agttatcagg cctcaggttc ctgatccggt accgggggta ccactacgcc ttcacgcggc
    27781 gcttcaggat tcggcgctgg cagattcatc aacttcgcca gaatgctcgc cagtttcagg
    27841 cgcatttccg gacgacggac gatcatgtcg atcgcgcctt tctcgatcag gaattcactg
    27901 cgctggaatc ctggcggcag tttttcgcga acggtctgtt cgataacacg cggaccggca
    27961 aagccgatta acgctttcgg ttcagcgatg ttgagatcgc ccagcatggc gaaacttgca
    28021 gaaacaccgc ccatcgtcgg gtcggtcagc acggagatgt acggcaagcc gcgctcctgc
    28081 atttttgcca gtgccgcaga ggttttcgcc atctgcatca gcgacatcag tgcttcctgc
    28141 atacgtgcgc caccagaggc ggagaagcag atcagcgggc agttatcttc cagcgcctgc
    28201 tcaacggcac gcacgaaacg tgcacccaca acagacccca ttgaaccgcc cataaaggcg
    28261 aactcgaatg ccgcagcgac aaccggcatt ccatacagag tgcctttcat caccaccagc
    28321 gcatcttttt cgccagtttc tttctgcgca gatgccagac ggtctttata cttcttggag
    28381 tcacgaaact tcagcacatc tttcggctca agctcgctac ccagctccac aaggcttcct
    28441 tcatctaaca ggctatgcag gcgattacgc gctgtcatac gcatgtgatg gtcacacttc
    28501 ggacagacct caagattacg ttccagctca gcgcggtata aaacctgacc gcagctatca
    28561 cacttagtcc acaccccttc aggaatgctc gccttgcggg tgggagtaat gttgctttta
    28621 attcgttcaa tccagctcat tagggacctt tctgtctgaa cctggttcga tgccagtttt
    28681 atctttgggg acgcataatg ccatttttgc ccccaacaga ccatgaatgt tgcacattaa
    28741 aacataacag cccgaaactt tggataaaaa agtggtcgaa ccgcggagtt actttttatt
    28801 ttgcggcgcg tgccgcagcg cgtttgtgac gaataaattc gaaaacccca ggcaatattg
    28861 aaacaaaaat aatgccaaca atcatcagct taaggttgct ctgaatgaag gggagcgtgc
    28921 cgaagaaata acccgcgtag gtaaaaatca gtacccacaa cagtgcgccg atcacgttat
    28981 aagcggcgaa atgacggtac gacatgtggc ccattcccgc aacaaacggc gcgaacgttc
    29041 tgacgatcgg tacaaaacgg gcgagaataa tcgttttgcc gccatgtctc tcgtaaaact
    29101 gatgagtctt atcaagataa ctgcgacgaa agattttcga atccgggtta ctgaacagtt
    29161 tgtcaccaaa taaccgtcca atggtatagt tcactgcatc accgacaata gcggcaatca
    29221 acataagcac caccatcata tggacattga gatcgttagt ttccagcgac gccaatgcac
    29281 cagcaacaaa cagcagcgaa tcaccaggca ggaatggcgt taccaccagc cccgtttcgc
    29341 agaacagaat caaaaacaga atggcataaa cccagacgcc gtactccgcg accagctctg
    29401 ccagatgcac gtcaatgtgc aggataaaat cgatgaggaa ataaatcagg tccatatttg
    29461 cctatgcctt gtactcgtca tctttcaggc tgtaactacg ttggctacag cctgaaatgt
    29521 tccgagcatt attctcgtta gtccgccaga aatagcgggc ccattggcgg ttttggaaga
    29581 tcataccggt cagggtaatc caccgcgacc agatacagcc cttccgcttt tgccgttgct
    29641 gccgcccgcg ttctgtcctt tgccgccagc aactctgcta tccagctctc cggctggttg
    29701 tgggcaccga cttccatcag gctgccgaca atattcctga ccatatgatg tacaaaggca
    29761 ttcgctttga tatctaccac cacataagga ccgtgacgca tgacattaat atgcataacg
    29821 ttgcgccacg gggttcggga ctggcactgc accgcacgga acgaggtgaa atcattctcg
    29881 cccagcaagc attgcgcagc ccgatgcatc cgttcagcgt ccagcggttc gtaaaaatgg
    29941 gttacccctt tactcagtac cgccgggcgc agccgatgat tgtagatgat gtagcgataa
    30001 cggcgagccg tggcgctaaa tcgggcatga aaatcatcag gtacagcttt aacccaacgc
    30061 acggcgatgt caccaggtaa attcgcattt acgcccaagg tccacgccgc gtctttgcgc
    30121 tgcgcggttg tttcgaaatg caccacttgc ccggtaccgt gtacccctgc gtcagtacgc
    30181 ccggcgcaga agacggtgat gggttcgttc gccacctggg agagcgcctt ttccagcttc
    30241 tcctgcacac tgcggacttc attctgccgt tgccagccgt aatacttact gccgtcgtac
    30301 tcaatgccca gcgcaatttt ataaactggc agttgttgct ggtcggacat tagtacagat
    30361 actcctgcac cagtttctcg gcgattttta ctgccatcag cgcgccgcca aagcgaacgt
    30421 tatcggccac cgaccagaac tggacttgct ccggcatacc gtagtcatta cgcacgcagc
    30481 caacagaaag atgcggcgta cccgaagcat cccctacctg agtcgggaat tcgttctctt
    30541 cagagagcac aatatcttcg ccctgagcaa acgcatcacg cgcttcttct gccgccagcg
    30601 gacgcagtgc ttcaaagttg accatctggg catgaccgta gaataccggt gcctggacga
    30661 cgcttgccga aatcatcagc ccttcgtcct gcaggatttt gcgtacttcg tcaacgatac
    30721 gacgttcttc acgcacgcta ccttcgctat ccggcaacaa cggcagcata ttgaacgcca
    30781 gttgacgacc gaagaaatct tcttcgtcaa tcggaatgcc gttgagcaat ttcgcactct
    30841 gccccgctaa cgcatcgacc gcttttttgc cctgggcgga ggctgaaatc aggctggtaa
    30901 cactgatacg cgacaaaccg ccctgatcga ttaacggttt cagtgccgcc agcagctggc
    30961 tggtcagact gtctggtacg gcgatgacat tccggttgcg gtaatcggtc agtacaaacg
    31021 ggtttacttc cggcaccacc agcggtacgt cgggttcgag agcaaacagt ccgctgctgt
    31081 cgatcaccag gcaacctgag ttggtcgctt cttcaaccca ggcagcggta gcttctttgc
    31141 ctgcgacaaa aaatgccagc tgcgcctgcg tccagtcgaa ttcagcggca tcctgcacgg
    31201 tgattgtttt accaccaaag cgcagttgtt cgcctgcgct ttcgttacgt gccagtgcat
    31261 aaatttcccc aaccgggaac tgacgttcag ccagcgtttc aagcagggct tcgcccacag
    31321 cgccagttgc gcccaggacg gcaatgttcc agccttcaga catggtggtt tactccagaa
    31381 atagcaaagc tccctgccaa tgttacagca gagagcatga agaagagatt aacgtgccgg
    31441 atgatgaacg gcgttaaaac ccagtttaca cagcaatgat gccgcactgg cgtcatcaca
    31501 aattacatac agagatgacc attcacggcg ctcaagatag tttttgcgca gtttatcgaa
    31561 ctcacccggt atcccggcga ctttacgcag cggtgcgtca tcgcggcgca catcatacac
    31621 caaatgcacc agccttttca gcgtcggttg atcgagcggg ccatgcagcg taatgcgacc
    31681 aaactctggc gcgggcagta atgtatccag cgcaacgtgc tgttcatgcc caataaactt
    31741 gctataggct tcaaacactt gcgtagtacc gcgtgcttta ccttccaggg tataacctgc
    31801 gatatgcggc gtgccgatat ccacttttgt cagcaactcc acgttaagtt ccggttcgcc
    31861 ttcccagaca tccagcacta cgcttaactt ctggccttca ttcaggcagg tcagcaacgc
    31921 agtattatcg acgactgcgc cacggcaggc gttaatcaga atcgctcccg gcttcaggct
    31981 acggatcagt ttttcatccg ccagatgcag cgttttgtac ggcccatctt tgaagagtgg
    32041 cgtatggaaa gtcagaatat ccgcatgctg aactaattca tccagcgagc ggaaatcacc
    32101 ctcatcccca cggtcagcgc gaggcggatc gcaaagcaat gttttaatcc ctaacgcttc
    32161 cagtcgcgcc tgcaatcgac gtccaacgtt accaacgccc acgatcccca ccgtacggtc
    32221 gtgcagtgaa aatccatcgc gttcggcaag catcagtagt gaggagaaaa catattccac
    32281 caccgcaatc gcattacagc caggtgcagc ggaaaaacca attcccgcct gcttcaacca
    32341 tgcttcatcg acatggtcgg tccccgcagt ggcagtgcca acaaatttaa tgggttttcc
    32401 tgccagcaaa gattcattca ctttcgtgac cgaacgcacc atcagcgcat ccgcgtctgc
    32461 cagttgagca acggggattg gacgcccggg aaccgcggtc acctcaccca aacggctaaa
    32521 taagtcgcgg gcataaggca tattttcatc aacaaggatt ttcacgtttg tgttacctgt
    32581 atgagacgag agttaaccgg acaagtgtgc cataatctcg cggccaggca tacttgcgaa
    32641 gatttcaggt ataaggatac gtaatgatac aacctatttc cggccctcct cctgggcaac
    32701 caccaggtca gggagataac ctgccgtctg gcgcgggcaa tcagccttta tccagtcagc
    32761 aacgtacttc gctggaaagc ttaatgacga aagtgacctc actgacgcaa caacaaagag
    32821 cagaactgtg ggcgggtatc aggcacgata ttggtctgtc gggagattca ccgctgcttt
    32881 cgcgtcactt ccctgccgct gagcataatc tggcgcaacg tctgctggcc gcgcaaaaaa
    32941 gccattctgc ccgccagctt ttagcgcaat taggggagta tttacgtctg gggaataatc
    33001 gtcaggcggt cacggattat atccgtcata actttggtca gacgccgctg aatcagctct
    33061 caccggagca attaaaaacc attctcaccc tgttgcagga agggaagatg gttattccgc
    33121 aaccacagca gcgcgaggcg accgaccgtc ctttattacc ggcggagcac aatgcgctaa
    33181 aacagctggt gaccaaactt gcggcggcaa cgggggaacc cagcaaacag atctggcaat
    33241 cgatgctgga actttccggg gtgaaagatg gcgagttaat tccagcgaaa ctgtttaacc
    33301 atctggtgac ctggttgcag gcgcgccaga cgttaagcca gcaaaatacg ccgacgctgg
    33361 aatcactaca gatggcgcta aaacaacctt tagatgccag tgaactggcg gcgttatcgg
    33421 catatatcca gcaaaaatat ggcctttctg cgcaatcatc gctttcttct gcccaggccg
    33481 aggatattct taatcagctt tatcaacggc gggttaaagg gattgatccg cgtgatatgc
    33541 aaccgctgct taatcctttt ccaccgatga tggacacgtt gcaaaatatg gcaacgcgtc
    33601 ccgcgctgtg gatactgtta gtcgcgatta tcctgatgct ggtctggctg gttcgttagc
    33661 cccgacgaaa tgacagtatc gtgacaataa ttcccagcac cgcagagatc gccccggcaa
    33721 gaaataccga agagtaacca aacgtggtcg ccagcattcc cgccagcggc ccggagacgc
    33781 cgagggcgat atcctgaaac gcggcgtaac cgcccagtgc ggtgccgcga acttgtgagg
    33841 ggacgcgttt aaccacctcc acgcccagcg caggaaagat aagcgaacat ccggctccgg
    33901 ttaacgccgc gcccgctaat gcgacccacg cacctggggc ttgccagagc agcaacaagc
    33961 ccaccgtttc tacaagcaga gagacaatcg ccactttcac gccgccaaaa cggtccggca
    34021 tccagccaaa catgacgcgc atcacgacaa atgcgccgcc aaacgcggta agagtaaagc
    34081 ccgccatcgc ccatcctttg ctggcaaagt agagcgaaac gaaagtcccg ataaccgcaa
    34141 aaccaacgcc ttgtagtgcc agccctaacc ctggtttcca gataaacccg acaacgctcc
    34201 acagcgatgg acgttctccc gccagggccg gtactttgcg cactgtgccg ttacaggccc
    34261 acgccagtaa gggtaatacc attgtggtga tcgccagtgc ggcaaaaccg taatggctat
    34321 gaatcaacag gccaagcgga gcaccaacag cgagggcacc gtaaatcgcc attccattcc
    34381 atgacatcac tttgccagag tgttttggcc ctacgatgcc taacccccag gtcagagcgc
    34441 ctgtcagtaa ctggctttca ccaaacccaa gaatcaaacg cccgatgacc aacagggcaa
    34501 atttgaacgg tgcggagaca ggcaaaatcg ccgccagcag caacgcgccg ccagccagac
    34561 cacaagctaa catcccctga agcgccgaac gttttgcacc atattgatcg gccagtcgcc
    34621 cggcatagcc acgcgtcagc accgtagcca gaaactgaat cccgacggca atcccgacca
    34681 tggtattgcc atagcccaga tcatgatgaa caaacagcgg gataaccggc aacggcagcc
    34741 ctacggtcat gtaggtgaga aaaaccgcaa aagcgatgcg gaagagcgaa aaattggcag
    34801 aagatcgtgt ttcggtttgg cttacagcag tcatgcatta ctccagaatg cagcgcaagg
    34861 cgaggagtat ccccgtctca tctctctggt ttcagggtta cggtgcgttg gcaggattta
    34921 acgcgtacgt cttttcagaa ggaaatcgac aaagcgggaa gtttgcctgg aacaggcggc
    34981 gattgtcaat gatgtgaaaa agggaaccat caggttccct tttgcgttag tgccggaggc
    35041 cgcattagcg cgtaacgtcg gatgcgacgc tggcgcgtct tatccgacct actccgacta
    35101 ctccggccta ctgcgaatta atctttcagc ttgcgcatta ccagcgtggc gttggtgccg
    35161 ccgaagccga agctgttaga cataacggtg gtcagttcgc gatcggtcgt ttcggtcacg
    35221 atgttcagac ctgcagcctg ctcgtccagc tcttcaatgt tgatgctcgg ggcgataaag
    35281 ccgtgttcca gcatcagcag agagtagata gcttcctgta cgccagcagc gcccagagag
    35341 tgaccggtca tggctttggt tgcagaaatc gccgggctct tatcgccgaa cacttcacgg
    35401 atagctgcca gttctttcac gtcgccaacc ggagtcgaag taccatgaga gttcaggtaa
    35461 tcgattgggg tatcaacgcc gtgcatcgcc atcttcatgc agcgtactgc gccttcgcca
    35521 gacggagcaa ccatgtctgc accatcagag gttgcgccgt agccaacgat ttcagcatag
    35581 atgtgagcac cacgcgccag cgcgtgttcc agctcttcaa ccactaccat accgccgccg
    35641 ccagcgataa cgaaaccgtc acggtgagcg tcgtaagtac gggaggcttt ttccggggtg
    35701 tcgttgtatt tagtagacag cgcacccatc gcgtcgaatt cgcaggccat ttcccagcac
    35761 agctcttcgc cgccgccagc aaacacgatg tcctgtttgc ccagttggat ctgctctact
    35821 gcgttaccga tacagtgtgc ggaagtcgca cacgcggagc tgatggagta gttaacgcca
    35881 tgaattttaa acggcgtggc gaggcaggca gaaacgccgg atgccatcgc tttggtgacc
    35941 acatacgggc caaccgcttt caggccgcgc gggccgcgca ttgcgtcagc gccgaacacc
    36001 tggaaacgcg gggaaccgcc gccagaacct gcaatcaggc caacgcgcgg gttattctgg
    36061 taagcttccg gagaaaggcc cgcatctgcg attgcctgct ccatagaaag gaatgcataa
    36121 atggatgcgt cgctcataaa gcgcacaact ttgcggtcaa tgaggccagt ggtatccagt
    36181 tttacgttgc cccagacgtg gctacgcatg ccggaatcct tcagctcctg agagaaagtg
    36241 atccctgaac gtccttcacg cagagatgcc aggacttcct gctggttatt accgatgctg
    36301 gaaacaatgc ccaggccagt aatcactgca cgtttcattc aatacctctg taagtcgcac
    36361 atagagtaag tttcgaatgc acaatagcgt acacttgtac gccgaacaag tccgatcagc
    36421 catttaatag agaaatttgc gcagccttac acacatcgct aagatcgagc caccgcctgt
    36481 aagacgagta acttacgtga aacactactc catacaacct gccaacctcg aatttaatgc
    36541 tgagggtaca cctgtttccc gagattttga cgatgtctat ttttccaacg ataacgggct
    36601 ggaagagacg cgttatgttt ttctgggagg caaccaatta gaggtacgct ttcctgagca
    36661 tccacatcct ctgtttgtgg tagcagagag cggcttcggc accggattaa acttcctgac
    36721 gctatggcag gcatttaatc agtttcgcga agcgcatccg caagcgcaat tacaacgctt
    36781 acatttcatt agttttgaga aatttcccct cacccgtgcg gatttagcct tagcgcatca
    36841 acactggccg gaactggctc cgtgggcaga acaacttcag gcgcagtggc caatgccctt
    36901 gcccggttgc catcgtttat tgctcgatga aggccgcgtg acgctggatt tatggtttgg
    36961 cgatattaac gaactgacca gccaactgga cgattcgcta aatcaaaaag tagatgcctg
    37021 gtttctggac ggctttgcgc cagcgaaaaa cccggatatg tggacgcaaa atctgtttaa
    37081 cgccatggca aggttggcgc gtccgggcgg cacgctggcg acatttacgt ctgccggttt
    37141 tgtccgccgc ggtttgcagg acgccggatt cacgatgcaa aaacgtaagg gctttgggcg
    37201 caaacgggaa atgctttgcg gggtgatgga acagacatta ccgctcccct gctccgcgcc
    37261 gtggtttaac cgcacgggca gcagcaaacg ggaagcggcg attatcggcg gtggtattgc
    37321 cagcgcgttg ttgtcgctgg cgctattacg gcgcggctgg caggtaacgc tttattgcgc
    37381 ggatgaggcc cccgcactgg gtgcttccgg caatcgccag ggggcgctgt atccgttatt
    37441 aagcaaacac gatgaggcgc taaaccgctt tttctctaat gcgtttactt ttgctcgtcg
    37501 gttttacgac caattacccg ttaaatttga tcatgactgg tgcggcgtca cgcagttagg
    37561 ctgggatgag aaaagccagc ataaaatcgc acagatgttg tcaatggatt tacccgcaga
    37621 actggctgta gccgttgagg caaatgcggt tgaacaaatt acgggcgttg cgacaaattg
    37681 cagcggcatt acttatccgc aaggtggttg gctgtgccca gcagaactga cccgtaatgt
    37741 gctggaactg gcgcaacagc agggtttgca gatttattat caatatcagt tacagaattt
    37801 atcccgtaag gatgactgtt ggttgttgaa ttttgcagga gatcagcaag caacacacag
    37861 cgtagtggta ctggcgaacg ggcatcaaat cagccgattc agccaaacgt cgactctccc
    37921 ggtgtattcg gttgccgggc aggtcagcca tattccgaca acgccggaac tggcagagct
    37981 gaagcaggtg ctgtgctatg acggttatct cacgccacaa aatccggcga atcaacatca
    38041 ttgtattggt gccagttatc atcgcggcag cgaagatacg gcgtacagtg aggacgatca
    38101 gcagcagaat cgccagcggt tgattgattg tttcccgcag gcacagtggg caaaagaggt
    38161 tgatgtcagt gataaagagg cgcgctgcgg tgtgcgttgt gccacccgcg atcatctgcc
    38221 aatggtaggc aatgttcccg attatgaggc aacactcgtg gaatatgcgt cgttggcgga
    38281 gcagaaagat gaggcggtaa gcgcgccggt ttttgacgat ctctttatgt ttgcggcttt
    38341 aggttctcgc ggtttgtgtt ctgccccgct gtgtgccgag attctggcgg cgcagatgag
    38401 cgacgaaccg attccgatgg atgccagtac gctggcggcg ttaaacccga atcggttatg
    38461 ggtgcggaaa ttgttgaagg gtaaagcggt taaggcgggg taatctgctc tcgcagtgtt
    38521 tgccggatgc ggcgtgaacg ccttatccga cctacgtgtg acgtgtaggc ctgataagac
    38581 gcgataagcg tcgcatcagg catggtgctc cagacgccgc aacttactgc tgtgacgcct
    38641 gctgaaacag gttttcccac atatcggtca ccagcgcctg gtcacgcggc gacaattcac
    38701 cggcaccaat ggctttttcc agactctggc taacggtcgt atgcaccgcc tgagcggagt
    38761 ggtcatcacc actttccagt tctgcgatgg ctaatgtcag gtggccacgc aaatacccac
    38821 tggcaaacaa ctcatcatca ctggcatggt caaccatacc gtcgattaat gccagaatgc
    38881 gtgattcaaa ctccgcgatc atcttctttc ctcattgtaa aacgtggcac cggcttcagt
    38941 tgagcgcttc cggccacggg aactgttccg ccgtaagttc cggcgtgtga taataatttt
    39001 gtagtgcttt aatgaagcgt gccgggcgtt caggaatgcc gtttgctagg taatccatca
    39061 cctgcgcatg tacccgccgt tgaaacacca cacgatccgg ttcgaagtcg ccttccagat
    39121 tgtcgcaact gacgttaaac ggatatcccg ccgccacaca gaacaaccag tcgaacgcct
    39181 gcggcttcac ttcaacatct tcaaactggc tttgcgtttg ggcatcgcgt ccgtccgggc
    39241 aataccagta gccgaaatca accagctcac ggcgcgcttt cccagcaata caccagtgcg
    39301 aaatctcatg aattgcgctg gcataaaagc catgagcaaa gacgattcgg ttatacggta
    39361 cttccgcatc agcaggaaga tagatcggtt cgtcgtcgcc tttaatcaga cgggtattaa
    39421 attcatcggc aaagcagcta ttaaaaattt caatcaactg ctcatagtgg tgtgtactgt
    39481 tcattagttc atccccaacc agtggaggat ctcctgtccg tggctatcaa aaagtaattt
    39541 ggcactcatc accgctgaga caataacaat catcgggcgg atcagctttt gtcctttgct
    39601 caacaccagt cgtgaaccca tgcgcgcgcc gaggaactgc cccaccagca tgacaaagcc
    39661 cgtcgcccaa atcactttgc caccgagaat aaacagcagt aaaccgccga tgtttgacgt
    39721 tgcgttgagt aatttggcgt gcgccgtggc tttggcgagg ttgaatccgc acagcgtaac
    39781 aaaggccagt gcgtaaaacg atccggctgc cggaccaaag aatccgtcat aaaaaccgac
    39841 gcagccaccg gcaattaacg cgaagggtaa accgtacatc cgacgctggc gatcttcttc
    39901 acccagcttt ggcatcagca agaaatagag gccgatacaa attaccagaa tgggcaaaat
    39961 ctgccgtaag acatcagcct gaacgtattg caccagcagt gcgccgctca ttgagccgac
    40021 aaaggtcatg gcgatattga gtttctgatc gcttaaacta accactttgc ggcgaataaa
    40081 gtagatagta gcggaaatag agccgccgca ggcttgcagt ttattggttg ccagcgcatt
    40141 agcgggagac atccccgctg ccatcaatgc cggaatggtg agtaacccac cgccaccggc
    40201 aatcgagtcg ataaatcccg ccagcatggc gacaaaaaag agaactccca gcaacagcgg
    40261 ggaaaccata aacaggctat taaacgtttc cattagatca cgtgctcatc cagtagcgcc
    40321 tggcaggaag gcggcaacgg aggcggtgtc ttcttctcag gctttgttgt tcccggtttt
    40381 ggaggttcaa accagctttg cagttctgcc ccgcaaccat cgcctggtgg cggtaaaggt
    40441 tgatcttcac actccagact atcggcagga caacgtaatc gtacatgcat atgcgcacga
    40501 tgctggaacc agggtcgcac tttgcgcaac cagtcgcgat cggtgcccgc atctaggcaa
    40561 agttgttgtt taatcgccgg attaacaaaa atgcgcgtga cgtttttgtc ctgggcggcg
    40621 agtttgatca agctgaaaat ttctggcttc cacagcgagg agacaacgtg tttaccgtcg
    40681 cgggaaacta agtccagtgc ttgcgggcgc aagagctgcg cggaggtcca gcgagttttc
    40741 ggcagttgca gaaagatatc gacatccagt ccggtctggt ggctggcatg accgccgttg
    40801 aaacgcccac cagcaggcat ccccatatcg ccaatcagca ccgtacccat gcccagattg
    40861 ctcacctggc tactcagacg ctggataaac atcaccagat ccgggtgacc gaaatagcga
    40921 cgctgatcgg tacgcatgac ctgataatgt tcggactgta tcggcagcgt gtcagcgccg
    40981 acaatacagc cattagaaaa actgcctatc gattgtgcgc tacccggcac aggttgggtt
    41041 attttttgcc acggcgtcgc tgccaggcta gcgctactgg caagcagagc cagcagcgca
    41101 atcgcggttt tgttcatttt ttaccagcgt ggaatatcag tcttcacatc ggcattttgc
    41161 gcccgctgcc gtaacaggtg atccattaaa acgatcgcca gcatcgcttc tgcgatcggc
    41221 actgcacgga tcccgacaca gggatcgtga cggcctttgg tgatcatctc aacttcttcg
    41281 ccaaagcggt taatagtgcg ccccggcacg gtaatgctgg aggtcggttt cagcgccata
    41341 tgggcaatga tttgctgccc gctgctgata ccgccgagaa tgccgcccgc atggttgctc
    41401 tggaaaccgt ctttggtgat ttcgtcgcgg ttctggctgc cgcgcagcgc cactacgtca
    41461 aaaccgtcgc caatttccac gcctttcacc gcgttgatgc tcatcagcgc atgggcgatg
    41521 tcggcatcca ggcggtcaaa gaccggctcg ccaagtccgg cggggacacc actggcaaca
    41581 acggtgactt tcgcgccgat ggagtcgccc tcttttttca gcgcgcgcat cagttcatct
    41641 aacgcgtcga ttttgtccgg gtccgggcag aagaacggat tttgttcaac cagcgaccag
    41701 tctttgattt ccagcggaat gtcgcccatc tgggtcaagc agccgcggat ttcaatacca
    41761 aatttctcgg cgagatattt tttggcaatc gcccctgccg ccacgcgcat ggcggtttcg
    41821 cgggcggaag aacgtccacc gccgcgataa tcgcgcagac cgtatttttg ttcgtaggtg
    41881 taatcagcat ggcctgggcg gaaaacgtct ttaattgcgc tgtaatcctg agagcgctgg
    41941 tcagtgtttt cgatcaacaa gccaatgctg gtgccagtag taacgccttc aaaaacaccg
    42001 gagagaattt tgacctgatc cggctcgcgg cgctgggtgg tatagcgcga tgtcccaggg
    42061 cgacgacggt cgaggtcatg ttgcaggtcc gcttccgtca gcggaatgcc tggcggaaca
    42121 ccatcgacga tgcagccgag cgccagccca tgcgattcgc cgaaggtggt tacgcgaaag
    42181 agttgtccaa ttgtgtttcc agccatcacg gctccgttat tgttgtgttt gcgtgtttac
    42241 ttaatcttta taaatcgcga aatattcacg ggccgccagc agctgctctt tggtgagcat
    42301 aaacacacca tcgccgccgt tatcaaactc cagccaggtg aacggaacat ccggatattg
    42361 ttccataaga tgtaccatgc tgttgccgac ttcacaaatc agcacaccat catcagcaag
    42421 gtaatctgcc gcgttaccga gaatgcgacg cgtcagtttc aggccgtcgg tgccagatgc
    42481 caggcccagt tccggctcgt gacggtattc gtttggcagg tcggacatat cttccgcgtc
    42541 gacatacggc gggttagtga caatcagatc gtattgcact ttcggcaaat cacggaacag
    42601 atcggaacga atcggaatga cgttgtggat cagaccgtgt tcttcgatgt tctgttcggc
    42661 aaccgccagc gcgtcgggag agatatctac cgcgtcgact tccgcttccg ggaaggcata
    42721 agcacaggca atggcgatgc agccgctacc ggtacacata tctaaaatat gctgcggttg
    42781 cttgctgatg agtccggcaa atttattgtt gatcagttca ccaatcggtg agcgcggcac
    42841 cagcacgcgt tcatcgacgt aaaattcatg accgcagaac caggctttgt tggtcaggta
    42901 agccaccgga atgcgctcgt tgacgcggcg gatcacgcgt tcaacaatac ggtgtttttc
    42961 gctggaggtc agacgcgcgg tgcgcatatc ttccgggata tccagcggca ggtagagcga
    43021 tggtaacacc agctgtacgg cttcatccca cgggttatcg gtgccgtgac cgtaccagat
    43081 atttgccgcg ctgaagcggc ttaccgacca gcgcaacatg tcctgaatgg tttgcagctc
    43141 atttactgct tcatcaacga aaattttatc cacgtattcc tccagggcat gatcgcaata
    43201 atttcggcgg ctagtttgcc atgaagatga cgataaatca gcattcacgc gcggtgagtg
    43261 aggaaaaata cgtttaaaac gatcgattgt gctacgagtc gggtaaactg taggaaaatt
    43321 agaaatagag acagataaat gaaaaagaaa acaacactta gcgaggagga ccaggctctg
    43381 tttcgccagt tgatggcggg gactcgcaag attaagcagg acacgattgt ccaccgaccg
    43441 cagcgtaaaa aaatcagcga agtgccggtg aaacgcttga tccaggagca ggctgatgcc
    43501 agccattatt tctccgatga gtttcagccg ttattaaata ccgaaggtcc ggtgaaatat
    43561 gttcgcccgg atgtcagcca ttttgaggcg aagaaactgc gccgcggcga ttattcaccg
    43621 gaattgtttt tggatttaca cggtctgacg cagctgcagg ccaagcagga actgggcgcg
    43681 ttgattgccg cctgtcgccg cgaacatgtg ttttgtgcct gcgtgatgca tgggcacggg
    43741 aagcatattt tgaagcaaca aacaccgctg tggctggcac aacatccgca tgtgatggca
    43801 tttcaccagg caccgaaaga gtacggcggt gatgcggctt tgttggtgtt gattgaagtg
    43861 gaagagtggt tgccgccgga gttgccctga ggagttgagc gtgttgtgcc aggagcgcgg
    43921 aatggcaggg gcgaatgacc ctgccaggtg agaaaaggtg cgctaataca tattcagtat
    43981 gtaagtgaat cggcaggtag aatctatggg ctgtcgagat gtcgtgtcca cgcgtagctt
    44041 catatcatgc agttgcctgg acgtgatcgg ttgcggctct ggcgtcaccg tcactttggc
    44101 attatcacag ccggaagtcg agaaggtcat ctgctgtgcg acaaaacggc agtaggctcc
    44161 acgctcgtca aaattactgc catcacacag tttgccgttt tccatcagga aagtgggcag
    44221 tgtttgctca ataacaccac ctgttgactg caacctgacg actttgttat gtacaacata
    44281 attttcattc caggaaacat catacggtgc cagggactct gtcgggcagt cagagctaac
    44341 ttttgttggt cctataaaaa ttccggcccc gaaacgctga tccatagtac tggcgctttc
    44401 aacataagac gacacaatcc agggacaacc gtgtaaatct ccatcgtaat aaacgccaag
    44461 tactaattca ttacatgacc aggccgttac tggagagaga gcatcatact cttctaatcc
    44521 ggggcaggta gaatccggta aggttgtatt attgcgctcc atcctgagtt cagggccggg
    44581 aggactcccg gtccaactaa ctaaaggcaa acttgctgcc gcagttgaat tagattcata
    44641 cgtgccatat tcaactgatt gaggggtcag gactgaaaga tgaaaacgat actttgcaac
    44701 aacgctggtc gtccccgcgg gtacatagag tgtggcgatc tttgtcgcag cctgtatccc
    44761 agaagcgcac agagccagca ccaataaaca tataacatga cgtattttat gcattttata
    44821 tatactcaaa cgtagctgtc aggctggcgg tgaaatcacc gatggttaca tcacgaccgc
    44881 ttttcgcctg gatccaggcc cggaaattaa tatcattgtt gccgttggta agcgcaaaag
    44941 tcgccccggt ggtgttgtta atatccaccc gcttgccgtc gagcgtttcc atccctatcc
    45001 ccacgccctg cgcggtggag gtgtcgtcga ctgccagaaa acccggctga ccgctgtctt
    45061 ccgttccggt gagggtgact ttgacgttgt atcccgccgg ccctttgcag tctttcagct
    45121 taaacacaac cggcagatgc gcggactgcc cggcgaccat caaatcccgg ttgctgacgg
    45181 tgggaaaatg aatttccgcc agattggcac catccaccac cagcgtgcag gatttactca
    45241 gcaggttgcc gtaaaaatgc aggttgttat cgactgcccg tgcccccgtg ctggcggtca
    45301 gcagcaggcc tgctaacagc gcataaccgg gtgaataaat gtgtgctgtt ttcattggta
    45361 atccacccgt aaggttgcgt aagcagaaaa attcgtgtcc gtcagcaccg catcagccgc
    45421 tttgaccggt accgcctcca gctttggctt ctggctaaaa tcggtggcgt taatggaaag
    45481 cggctcgttg agcttaaaac gctgaccgtc atgctgaagc tcaattccga agcccggtac
    45541 atccgtctca atagcagagt cgttgtagga ggtggcagtc cccgtccagg agagtgtcat
    45601 attccactcg ggatcgcgga catccgagtc gcagctcagt tcgtagtcca ctttctgacg
    45661 accataattg ccatcaatgc tgtcgataat aagatcgcga aattgcactt cgatggtttg
    45721 cccgccgctg atgctacagg cgggcggcga catcagcgta ccgtggaatg tgatgtcatc
    45781 ttccgccgcc agcacagccc cgcaaccagc gcacagtaaa aggagcagga attttttcat
    45841 tggtaatcca ccaccatggt catcgtggcg ttaaactccg acggcgtcag ttgcaccccg
    45901 ctctgtttta ccggcaccgc tttcagctga ggctgagtgt tgacattaaa aggcgtccag
    45961 ttattttcac caactgcaaa cagggagttg tcggcggcgt tttctatccg cacccccagg
    46021 ccggcgatat ccgtgctgag aaccgtttct ccgttgatca cggcggtatt gcctttgagc
    46081 tgcatccgta agtcatcgct gactttatta ccgcagttga gggtgtattt cgcatcctgt
    46141 tggtaattgg taccatcgat cctgttgatt atcacattgc caaatttcac agcgcttccc
    46201 ttcacggagc aaggcggcgg tccatcgacc aggattcgca gcgtcagatc cacctgtttg
    46261 atatcactgc cagcaacagc caaaccgctg atgccccatc cggccagcac cagcaaccct
    46321 gcgcccgcta caaacttgtg actcataagt ccctcccggc atcagccttt gcggctctgc
    46381 tcttcatcca cctggcagct gttgccggtg catatgaagt acagcggcat ccgcgcgccg
    46441 tagtcgttga tgtaagtcag caccggcacg ctgtccatct tcgcattcag cggtgcggag
    46501 gttttcggct ccatcatcag cggtgtgaat cccgccggcg ggttgccgcc ttttttagcg
    46561 ctggcgttgc tgatgatcac gtagtacggc gtcgggttat tgacggtgaa gtcctggccg
    46621 ttacggatga gcgtcacctt atgctgccac ggacttttca tgctggtgtt ctccagcgcc
    46681 ttcggtcgcc agaacagttt gatacgcgtc tgcagcgcaa tctgcagtgt gttcggttta
    46741 tttgatttcg gcggaatttc gcgcacgttg aaatagaaca cgctctcgcg gtcggccggc
    46801 agtttgttga tatccggcag cccctgcacc ttcacctggc cgttcatcat cgcatcgata
    46861 cgctgcactg gcggaagcac cgcaagcggc gagctgattt tgttgccctt tgcatcctct
    46921 atccagctct gcgccagata aggcagtgtg ggatcgttgt tgcgcagcgt gacgctgatg
    46981 gacttgtcgc tttcgttaaa caccagccgt gtcctgtcgg gcgtcaccga agcgtggctg
    47041 gagaaactga ccactgtcgc cagcagcgcc agtgccggga caacgatttt tgcagtgtta
    47101 gtcagattaa acatggatca ttccttaatc acgttactgg tttactgaaa ttgaagtcgg
    47161 aggttccgtt ggcgtcaccc gctgtgtctg ttcctgaatc gccggtttga ccgccggcga
    47221 agtgataggt gctacattgc ctttatgctg gcatggcagt aacaagccgt taaacagatc
    47281 ggcaggcagc gggtccggca ggttgatatc gcaatgcgca acaccactcc agaacacact
    47341 catgtgttcg ccaggtttca ccccagccag ataaacattg ccgtcatcat cgacaaggcc
    47401 cactgtctgc tcgttatcat tttttacttc tgcgccaaac ggaggatggc tgccgtcgct
    47461 caggcgcagc accgccatcg ctttttgtcc gctaatgaca gcaaatttac ggtagccaat
    47521 ggcaccttca gttagcgtgg cttgcaccac cgactgggtt gcttcagcgt tttcaggcaa
    47581 tctgttgagg tcgatatacg cctgattgcg gtaatagtta ttgacgtcag aaacgacggc
    47641 tttaccaaac atattggtat aaacagcagc cccgttacct tccaccggaa catcggcaac
    47701 gccatcggca tcaatcaaca atcgtgtccc gcccatattc tgggtacggt gaagtgcgcc
    47761 accgtgggca gtaagcgttg cgccgccctg taacgacaag cccgcagagg tgtattgccc
    47821 ttcatggtag ttcgcactga ggtcaacctg cgccagcgaa ccatcatggc tgtaatagcc
    47881 gtcaacgctg gtgtgtttgt cactggtgcc gacgttcaac tgatagtgag tcgcgtcatc
    47941 gacacggctg aaataaccga cctgactgct gtccgtccca ctgccatagt taccgttata
    48001 gctaacggta ctgttgtcgc cccacggcat actgagcgaa atgtacatgc ctttgtcggc
    48061 ctggttgtca tactcgtaac ggtagccagt catcgagatg ctgacattac gaatactgcc
    48121 catattgaaa tagtgcgaga gcatgatgtt gtagtttgtc tgctcctcgc gatcccagta
    48181 ggtatggcgg gtatagttga gataaaccga aacacccaca tcgcggaagt tctggttata
    48241 agtggcggtg tacatctctt tgtcgttgcc cgtgcgtacc attccgctgt cactggcatc
    48301 cagatactcg ctcatggtca taaagttctc ttccgagaag cgatatccag caaaagtaac
    48361 gcggctgttg agctggtcaa agtctttgga ataactcaca cggaaggagt taccgtccag
    48421 cgaacctttg ccataagcgg tgtctttatc cagtttggtg tgcgagtgag taacatcaaa
    48481 cgcgaccgcg ccgaatgtag acaaatcgcg accgacgcca agcgccgcag actgatagtt
    48541 ttcatctccc agtgcgccgc catataacga ccagccgtta gcgatccccc aggaagcttc
    48601 agcaccagaa aaaaattcac cctcgatatg gtgtccccac tcttgcggac ggcccatcat
    48661 gatcttatag cgaacctgac ctgggcgagt gaggtatggc atcgaggcgg tgctgatgtc
    48721 atattcctgc acctggccgt tctgttcttc aatgcgaata tgcaacgtac cggagacaga
    48781 atcaccaaga tcctgaatac gaaacggtcc agccggaacc tgcgtttcgt aaatcacacg
    48841 ccccatctgg ctgacggtca cttttgctgt ggtgtgtgcc acgccggaaa tgtctggcgc
    48901 gtagccgcgc agattgggag gcaacatttg atcgtcagta ctgacactgc caccaacata
    48961 gttaaagcca tcaaaaatat cggatctgag gtaatcctcg cccagcgcca gtttggcttt
    49021 taatgatggt aacgcccgcc aggcatagta gcgactccac tcccattttt tttgagtttc
    49081 atcgccgctg aattcatcgt catcatcatt actgcgagta tgttgatagt tagtctgcca
    49141 gtcagcacgc atacgccacg gccccaggtt aaccccgacc gtcccgttgc cgctgatctc
    49201 gttactatca tcaccgccat tttcttcgtg ccgggtttgt gcgttgatgc tgtagtccgc
    49261 gacgatcccg gagatgccgt catcccaacg tgaaggcgga tcccagtcgg gataagtata
    49321 ttcgaggtaa gcctgtggca gtgaaatgac taatgcggac tggcttaaat cagccttaat
    49381 ttccatgcct tccagttgac cggatttcag gcatttagca tcgtggctcc attgcagatt
    49441 tttcgccacg tcttctttta aaccaaactg cgctaccagt tccggtgtca gacaagcata
    49501 gcttttgctc gcgtcatctt caccagcata ccagtaaata tcgtactctt ccgccaatgg
    49561 ctgtttattt agttgaacct gtaaattgta ttttccgggc tcaacatatc cctggctgga
    49621 aaaacgcttc agatcaattt ttgtgtcgcc ttttaattcc agaaaacggg aatcaaactg
    49681 aatgtcgtct tcagcccaga cactgctata actccctgac attgccagcg caatgcacca
    49741 cggaagaatc cgtaatcgaa aaagagaatg gtcaggcata cacgtttcta tccgttttta
    49801 ttcttgttcg gtcaaaatcc atttaaatcc cccatagcag tgtcgctatg ggggtatgcc
    49861 ttacaggtaa gtaatctgga aggtggtatt tgcttcgaaa ttacccagat ctggcgcatc
    49921 agctgcgccg accagccagg ctttaaagtt cagtgtctgc ttcgctttgc ctttattggt
    49981 agaagtgtct ttcactattt tctggtcaat acccatgccg cttttgtaag aggtacccag
    50041 agagtcaccg atcgccaggc taacattgtt aaatgccgca ccggtatcgg tattgaaaat
    50101 ggtgtaataa ttgccgctat ttgcagaaga aacggtacca gtaaaggtag tggtcattgt
    50161 ttcctgagta tcaaatacgc aatcttgcag acgaatctgg aaacttttcg gcgtggtggt
    50221 gccgttattt ttcagttttt cggcaccgat atcacccata tcaacttcca gtttgtgatc
    50281 gtccggaacg atagaacaca ctgagtcttc aatggtgccg taaaaacgtg cagtaccgtt
    50341 gttacccgca gcgattgttg ccgtagaggt gaaaacgccc atcaccattg ccgcagcaat
    50401 agctgtttta acaaacttac tcattatttg tccttaaact ataaataatt aaaaatataa
    50461 acattacaat acattgattc agtcaatacc caatgtttat acccagccat tgaatacggc
    50521 atgcgatcca aaaagaacag gtcgtagaaa attgtattct ttggtgtggg gccgcatagt
    50581 gatgttttca cccccctgat gcaatttaat tttactaaag agtaaatgta gatttaatta
//
LOCUS       DAZFWL020000020        46093 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFWL020000020 DAZFWL020000000
VERSION     DAZFWL020000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 46093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.020"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            40..1620
                     /locus_tag="ACHKNP_RS06435"
                     /old_locus_tag="pgaptmp_001287"
     CDS             40..1620
                     /locus_tag="ACHKNP_RS06435"
                     /old_locus_tag="pgaptmp_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGU4703486.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMGK
                     FIPVLAIPLIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKEKDDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLFIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRRAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGLANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPLAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEETAIANIPVHDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEAAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIC"
     gene            1620..2828
                     /locus_tag="ACHKNP_RS06440"
                     /old_locus_tag="pgaptmp_001288"
     CDS             1620..2828
                     /locus_tag="ACHKNP_RS06440"
                     /old_locus_tag="pgaptmp_001288"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGU4703487.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNVACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            2797..3393
                     /locus_tag="ACHKNP_RS06445"
                     /old_locus_tag="pgaptmp_001289"
     CDS             2797..3393
                     /locus_tag="ACHKNP_RS06445"
                     /old_locus_tag="pgaptmp_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGU4703488.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            3465..4412
                     /locus_tag="ACHKNP_RS06450"
                     /old_locus_tag="pgaptmp_001290"
     CDS             3465..4412
                     /locus_tag="ACHKNP_RS06450"
                     /old_locus_tag="pgaptmp_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGU4703489.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
     gene            complement(4492..4568)
                     /locus_tag="ACHKNP_RS06455"
                     /old_locus_tag="pgaptmp_001291"
     tRNA            complement(4492..4568)
                     /locus_tag="ACHKNP_RS06455"
                     /old_locus_tag="pgaptmp_001291"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4532..4534),aa:Met,seq:cat)
     gene            4777..5874
                     /gene="mltA"
                     /locus_tag="ACHKNP_RS06460"
                     /old_locus_tag="pgaptmp_001292"
     CDS             4777..5874
                     /gene="mltA"
                     /locus_tag="ACHKNP_RS06460"
                     /old_locus_tag="pgaptmp_001292"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HGU4703490.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            5951..6757
                     /gene="tcdA"
                     /locus_tag="ACHKNP_RS06465"
                     /old_locus_tag="pgaptmp_001293"
     CDS             5951..6757
                     /gene="tcdA"
                     /locus_tag="ACHKNP_RS06465"
                     /old_locus_tag="pgaptmp_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HGU4703491.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(6808..7251)
                     /gene="csdE"
                     /locus_tag="ACHKNP_RS06470"
                     /old_locus_tag="pgaptmp_001294"
     CDS             complement(6808..7251)
                     /gene="csdE"
                     /locus_tag="ACHKNP_RS06470"
                     /old_locus_tag="pgaptmp_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HGU4703492.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAIEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(7251..8456)
                     /gene="csdA"
                     /locus_tag="ACHKNP_RS06475"
                     /old_locus_tag="pgaptmp_001295"
     CDS             complement(7251..8456)
                     /gene="csdA"
                     /locus_tag="ACHKNP_RS06475"
                     /old_locus_tag="pgaptmp_001295"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HGU4703493.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQLGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLNIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            8648..8875
                     /gene="ygdI"
                     /locus_tag="ACHKNP_RS06480"
                     /old_locus_tag="pgaptmp_001296"
     CDS             8648..8875
                     /gene="ygdI"
                     /locus_tag="ACHKNP_RS06480"
                     /old_locus_tag="pgaptmp_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HGU4703494.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9226..10143
                     /gene="gcvA"
                     /locus_tag="ACHKNP_RS06485"
                     /old_locus_tag="pgaptmp_001297"
     CDS             9226..10143
                     /gene="gcvA"
                     /locus_tag="ACHKNP_RS06485"
                     /old_locus_tag="pgaptmp_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HGU4703495.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            10162..10557
                     /gene="ygdD"
                     /locus_tag="ACHKNP_RS06490"
                     /old_locus_tag="pgaptmp_001298"
     CDS             10162..10557
                     /gene="ygdD"
                     /locus_tag="ACHKNP_RS06490"
                     /old_locus_tag="pgaptmp_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HGU4703496.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            10550..11650
                     /gene="rlmM"
                     /locus_tag="ACHKNP_RS06495"
                     /old_locus_tag="pgaptmp_001299"
     CDS             10550..11650
                     /gene="rlmM"
                     /locus_tag="ACHKNP_RS06495"
                     /old_locus_tag="pgaptmp_001299"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HGU4703497.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(11694..12425)
                     /gene="fucR"
                     /locus_tag="ACHKNP_RS06500"
                     /old_locus_tag="pgaptmp_001300"
     CDS             complement(11694..12425)
                     /gene="fucR"
                     /locus_tag="ACHKNP_RS06500"
                     /old_locus_tag="pgaptmp_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HGU4703498.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12483..12905)
                     /gene="fucU"
                     /locus_tag="ACHKNP_RS06505"
                     /old_locus_tag="pgaptmp_001301"
     CDS             complement(12483..12905)
                     /gene="fucU"
                     /locus_tag="ACHKNP_RS06505"
                     /old_locus_tag="pgaptmp_001301"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HGU4703499.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(12907..14325)
                     /gene="fucK"
                     /locus_tag="ACHKNP_RS06510"
                     /old_locus_tag="pgaptmp_001302"
     CDS             complement(12907..14325)
                     /gene="fucK"
                     /locus_tag="ACHKNP_RS06510"
                     /old_locus_tag="pgaptmp_001302"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGU4703500.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(14434..16209)
                     /gene="fucI"
                     /locus_tag="ACHKNP_RS06515"
                     /old_locus_tag="pgaptmp_001303"
     CDS             complement(14434..16209)
                     /gene="fucI"
                     /locus_tag="ACHKNP_RS06515"
                     /old_locus_tag="pgaptmp_001303"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGU4703501.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQRRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(16242..17558)
                     /gene="fucP"
                     /locus_tag="ACHKNP_RS06520"
                     /old_locus_tag="pgaptmp_001304"
     CDS             complement(16242..17558)
                     /gene="fucP"
                     /locus_tag="ACHKNP_RS06520"
                     /old_locus_tag="pgaptmp_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HGU4703502.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            18105..18752
                     /gene="fucA"
                     /locus_tag="ACHKNP_RS06525"
                     /old_locus_tag="pgaptmp_001305"
     CDS             18105..18752
                     /gene="fucA"
                     /locus_tag="ACHKNP_RS06525"
                     /old_locus_tag="pgaptmp_001305"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGU4703503.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            18780..19928
                     /gene="fucO"
                     /locus_tag="ACHKNP_RS06530"
                     /old_locus_tag="pgaptmp_001306"
     CDS             18780..19928
                     /gene="fucO"
                     /locus_tag="ACHKNP_RS06530"
                     /old_locus_tag="pgaptmp_001306"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HGU4703504.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(19983..20738)
                     /gene="xni"
                     /locus_tag="ACHKNP_RS06535"
                     /old_locus_tag="pgaptmp_001307"
     CDS             complement(19983..20738)
                     /gene="xni"
                     /locus_tag="ACHKNP_RS06535"
                     /old_locus_tag="pgaptmp_001307"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HGU4703505.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(20850..22217)
                     /gene="sdaB"
                     /locus_tag="ACHKNP_RS06540"
                     /old_locus_tag="pgaptmp_001308"
     CDS             complement(20850..22217)
                     /gene="sdaB"
                     /locus_tag="ACHKNP_RS06540"
                     /old_locus_tag="pgaptmp_001308"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HGU4703506.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(22275..23564)
                     /gene="sdaC"
                     /locus_tag="ACHKNP_RS06545"
                     /old_locus_tag="pgaptmp_001309"
     CDS             complement(22275..23564)
                     /gene="sdaC"
                     /locus_tag="ACHKNP_RS06545"
                     /old_locus_tag="pgaptmp_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HGU4703507.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(24121..25485)
                     /gene="ppnN"
                     /locus_tag="ACHKNP_RS06550"
                     /old_locus_tag="pgaptmp_001310"
     CDS             complement(24121..25485)
                     /gene="ppnN"
                     /locus_tag="ACHKNP_RS06550"
                     /old_locus_tag="pgaptmp_001310"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HGU4703508.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(25597..26445)
                     /gene="queF"
                     /locus_tag="ACHKNP_RS06555"
                     /old_locus_tag="pgaptmp_001311"
     CDS             complement(25597..26445)
                     /gene="queF"
                     /locus_tag="ACHKNP_RS06555"
                     /old_locus_tag="pgaptmp_001311"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HGU4703509.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            26513..27058
                     /gene="syd"
                     /locus_tag="ACHKNP_RS06560"
                     /old_locus_tag="pgaptmp_001312"
     CDS             26513..27058
                     /gene="syd"
                     /locus_tag="ACHKNP_RS06560"
                     /old_locus_tag="pgaptmp_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HGU4703510.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            27680..28009
                     /gene="yqcC"
                     /locus_tag="ACHKNP_RS06565"
                     /old_locus_tag="pgaptmp_001313"
     CDS             27680..28009
                     /gene="yqcC"
                     /locus_tag="ACHKNP_RS06565"
                     /old_locus_tag="pgaptmp_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HGU4703511.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            28009..28791
                     /gene="truC"
                     /locus_tag="ACHKNP_RS06570"
                     /old_locus_tag="pgaptmp_001314"
     CDS             28009..28791
                     /gene="truC"
                     /locus_tag="ACHKNP_RS06570"
                     /old_locus_tag="pgaptmp_001314"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HGU4703512.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            28809..29258
                     /gene="yqcA"
                     /locus_tag="ACHKNP_RS06575"
                     /old_locus_tag="pgaptmp_001315"
     CDS             28809..29258
                     /gene="yqcA"
                     /locus_tag="ACHKNP_RS06575"
                     /old_locus_tag="pgaptmp_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGU4703513.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            29693..31045
                     /gene="gudP"
                     /locus_tag="ACHKNP_RS06580"
                     /old_locus_tag="pgaptmp_001316"
     CDS             29693..31045
                     /gene="gudP"
                     /locus_tag="ACHKNP_RS06580"
                     /old_locus_tag="pgaptmp_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HGU4703514.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            31047..32387
                     /gene="gudX"
                     /locus_tag="ACHKNP_RS06585"
                     /old_locus_tag="pgaptmp_001317"
     CDS             31047..32387
                     /gene="gudX"
                     /locus_tag="ACHKNP_RS06585"
                     /old_locus_tag="pgaptmp_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HGU4703515.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            32408..33748
                     /gene="gudD"
                     /locus_tag="ACHKNP_RS06590"
                     /old_locus_tag="pgaptmp_001318"
     CDS             32408..33748
                     /gene="gudD"
                     /locus_tag="ACHKNP_RS06590"
                     /old_locus_tag="pgaptmp_001318"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HGU4703516.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGKYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(33980..36736)
                     /gene="barA"
                     /locus_tag="ACHKNP_RS06595"
                     /old_locus_tag="pgaptmp_001319"
     CDS             complement(33980..36736)
                     /gene="barA"
                     /locus_tag="ACHKNP_RS06595"
                     /old_locus_tag="pgaptmp_001319"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HGU4703517.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            36793..38094
                     /gene="rlmD"
                     /locus_tag="ACHKNP_RS06600"
                     /old_locus_tag="pgaptmp_001320"
     CDS             36793..38094
                     /gene="rlmD"
                     /locus_tag="ACHKNP_RS06600"
                     /old_locus_tag="pgaptmp_001320"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGU4703518.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            38142..40376
                     /gene="relA"
                     /locus_tag="ACHKNP_RS06605"
                     /old_locus_tag="pgaptmp_001321"
     CDS             38142..40376
                     /gene="relA"
                     /locus_tag="ACHKNP_RS06605"
                     /old_locus_tag="pgaptmp_001321"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HGU4703519.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            40454..40702
                     /gene="mazE"
                     /locus_tag="ACHKNP_RS06610"
                     /old_locus_tag="pgaptmp_001322"
     CDS             40454..40702
                     /gene="mazE"
                     /locus_tag="ACHKNP_RS06610"
                     /old_locus_tag="pgaptmp_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HGU4703520.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            40702..41037
                     /gene="mazF"
                     /locus_tag="ACHKNP_RS06615"
                     /old_locus_tag="pgaptmp_001323"
     CDS             40702..41037
                     /gene="mazF"
                     /locus_tag="ACHKNP_RS06615"
                     /old_locus_tag="pgaptmp_001323"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HGU4703521.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            41108..41899
                     /gene="mazG"
                     /locus_tag="ACHKNP_RS06620"
                     /old_locus_tag="pgaptmp_001324"
     CDS             41108..41899
                     /gene="mazG"
                     /locus_tag="ACHKNP_RS06620"
                     /old_locus_tag="pgaptmp_001324"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HGU4703522.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            42127..43764
                     /gene="pyrG"
                     /locus_tag="ACHKNP_RS06625"
                     /old_locus_tag="pgaptmp_001325"
     CDS             42127..43764
                     /gene="pyrG"
                     /locus_tag="ACHKNP_RS06625"
                     /old_locus_tag="pgaptmp_001325"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HGU4703523.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            43852..45150
                     /gene="eno"
                     /locus_tag="ACHKNP_RS06630"
                     /old_locus_tag="pgaptmp_001326"
     CDS             43852..45150
                     /gene="eno"
                     /locus_tag="ACHKNP_RS06630"
                     /old_locus_tag="pgaptmp_001326"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HGU4703524.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(45210..46082)
                     /gene="ygcG"
                     /locus_tag="ACHKNP_RS06635"
                     /old_locus_tag="pgaptmp_001327"
     CDS             complement(45210..46082)
                     /gene="ygcG"
                     /locus_tag="ACHKNP_RS06635"
                     /old_locus_tag="pgaptmp_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU4703525.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
BASE COUNT        11573 a        10885 c        12090 g        11545 t
ORIGIN      
        1 tggactaatt atacataaca ttttcatgag ataaccagta tgaaacaaaa gaaagcctgg
       61 agtttttttc agagcctggg gaaggcattt atgtatccca tcgctctgtt aagtgtatgt
      121 ggtatgatgt tagggctggg aagtggttta gccagcgatg atatgggaaa attcattcca
      181 gtgttggcta ttccgttaat taaaaccata cttgatttta ttgttagcct tggtttgttt
      241 gccttcgtta atttacctgt attgtttgcg atagctatac ctttgggatt attaaaagag
      301 aaagatgata aagcctatgg cgctttttct ggcttaattg gctttatggc gatgcatctt
      361 ggaacgaact tttatcttaa acagcacgac ttattggtag ctgctgacca aatgtcgaca
      421 catgggcaaa ccatcatact cgggatccag tcctacaata ccagcgtgtt gggtgggatt
      481 gtggcagggt tattagtcgc cagcatgtat aaaaagatcg ttaacttacg tattcctgaa
      541 tcattagggt tttatagtgg cccacgcctt gtgcctatca ttacactgat tgtgatgagt
      601 ggatttggtc tgttcattcc ttttatctgg ccgccatttt tcaatctttt catgctcatt
      661 ggtcactgga tttcaacttc cggtcctgtt ggttatttct tctatgctgt tgccgaacgc
      721 gtgacgatac cttttggctt aaatcacttg gtgacatcag ttttccgctt taccccaatc
      781 ggcggatcgg cagtgattgg cggtgaagag tattacggca ccctgaacat gtttatggca
      841 tacgtcaaag agaatgcggt cattccgctg gatttggcgg ggaaaatgga gcagggcaaa
      901 ctgatgattc agtatggtct ggctggagcc gcgctggcga tgtatcgcac tgctcatgct
      961 caaaacagaa gggctatcaa agcattgctg atttccggtg tgctcacggt aattattggc
     1021 ggcgtcagcg agccgattga atttctgttc ttatttgtca gcccgctgtt gtttgtcttc
     1081 catgccttta tgaatggatt ggctaacatg gttctgccat atatgggcgt gaagatgggc
     1141 tttactggcg atctgattca atttattagc tttggtgtct tacgtggcac aagaacggga
     1201 tggccattag cggtatgtgt tgaagtggct tatttcttca tttattactt tgtgttccgt
     1261 tggaccattc tcaaatttaa cctgatgact gtaggccgtg aagagtccag tcctgtcacg
     1321 ctgaacgctc acgaagaaac tgcaatagct aacatcccag ttcatgataa atcagagttg
     1381 caggcagcgg agcaaatggt taaggctctg ggtggtaaag aaaatattaa atcactggat
     1441 aattgtgtaa ctcgtttgcg tttaactatc gcggatatgg gattgataga tgaggctgca
     1501 ataaaaaggg ccggaggaat tgcggttgtg aaacttgatc aaaatacctt acaagtcatt
     1561 atcggtacaa aagtcatcgc cctacgtcgg gatatggata attacatggg gatatgttga
     1621 tggatatttt taatacctca atttctcgta aaggaacata ttgcacacaa tgggactttt
     1681 gtgaagacag atttggtgta aaagatgtat taccattctc tatttcagat atggatcttc
     1741 cgataccgga agctatcatc agaacattaa aaaaacgtct ggaacacccg attttagggt
     1801 acagtcgttg gcagcatgat gactatctga attcgatagt caattggtat catcaacaat
     1861 ataaaacaga gattcaacca gaatggatca cctatagccc aagcgtgatg tattcaatat
     1921 ctaaggcaat agaaatattg acattgaatg gagataatat tctcattttc acgcctgttt
     1981 ataacgcatt ttttgatgtg attaagcata atggtcgaaa catgctaaca acctctttga
     2041 tgaaagaaaa tgggagaggc tacgttatca actggcaaga ctttgagctt aaaattaaaa
     2101 gcgcgaagat ggtgttatta tgtaatcctc ataacccgac aggaacggtc tggtcggaag
     2161 acgatcttca cagaatcgca gcatgctgtg caaggcataa tatttggtta tgttcagatg
     2221 aaatacatag tgattttgtt tttaatcata gtttcacgtc agcgctaaca ataaaagatg
     2281 acaaagtggt ggtgtttaat tcgatttcga aaacatttaa tgttcctgcg ctaaccggat
     2341 catatatgat ttcaactgac gataatttta atcataagtt cagaacgata tcgaggtatc
     2401 gcgatttcgt aaactcccca tcagtactga atgtaattgc gaccattgtt gcctataatg
     2461 aatgtgaaga atggttaaag tcgttaaagg tacatattgc ctctaacatt caatttacct
     2521 tgcagtattt aaatgaaaat attccggaat tgatcgtatg gccagctgag ggctgctatt
     2581 tttcgtgggt tgattgctcg gcccttggtt atccctttga taaattttac cgtcgattaa
     2641 ttaacgaagg gagagtaggt attatggcgg ggcatgttta tggtactgaa ggagagggct
     2701 atctgcgtct taatgtggcc tgcagtcgag aaaaattata tatggggctt gcgcgccttg
     2761 cgagtgtaat taaaaatatt aatcaggaaa aagaaaatga acaatacgga tcttatccat
     2821 cttattaaac gttttatgcg taacgaacat aaagcagtag aagaggttat tgattccccc
     2881 ctttcggaag tcgcgaattt aattaaaata ttacaatctt gccagggaaa agttgttttt
     2941 attggtgttg ggaaatctgg cattattgcc agaaaacttg ctgcaacatt tgccagcacc
     3001 ggaactccct cgttttttgt tcacggtacg gaagcggtac acggcgacct tgggatggtg
     3061 gcgaaagacg atgttgttat tcttatttca aacagtggtg agacggcgga aatcctggcg
     3121 acactgccta gtttgaaaaa aatgggtaat tatttgatct cttttacccg cagtcatcac
     3181 tcatcgcttg cgataagttg tgatttatct gttgaaatcc ccgtcaaaag tgaggcggat
     3241 aatttaggtt tagcaccgtc atgctcgtct actgttgttc tggttgttgg tgatgcggtg
     3301 gcgcttgcac tctctgaatt gaaaaaattt actcgtgctg atttcggctt atatcatcca
     3361 ggaggtgcac tcggcattaa agcaaattca taatttcaaa gtataaatac agtaaccggt
     3421 cacgccatct tttggggtgg cgttcattga agtgagtgtc agctatgaaa aatgttctgg
     3481 tcaccgtccc cagcttttca gcacgctgtg tttccgccag caagttgcta agagagaata
     3541 attttaatct tattattaag aataatgtgg agcatctgct taaatcagaa tccactgcat
     3601 tacgagaatc tatttgcgcg gtcatcgccg gaaaagattg ttatcaggcg gacacgctat
     3661 cattgctgcc tggtgtgaaa ataatatcga gattcgggac ggggatagat aatattgatc
     3721 ttcgtgcagc acaacaatcc ggtattgtag tcaataatgc ggttgggatt aattcaaatg
     3781 ctgtcgcgga atttatcatt ggacttatct tcgcgagcat gagaaatatt ccaggcagct
     3841 atcatgcgat gcaaaatggc tactggggtg agtcgcatgg ctatgaatta caaggcaaac
     3901 ggattggcat ggttggctac ggcaatatag gtaaaaccct ggcaaaaagg ttatccggtt
     3961 ttgatgttga gcttttagct tatgacaaac aacccgatta tcaggttgct gacaaagttg
     4021 gagttcagtt tgtatcaatt gaagatatct ttatgcagtc gcatgtcatc attgttcttt
     4081 tgcctttttc tcctgagctg gagaacttca ttagtcataa atatttgtcg atgatgcgca
     4141 atggtgctct aatcatcaat gccgcaaggg gaaagttgct ggatgagggc gctttactac
     4201 aggtgattga agaacgaaat gtgtttgcgg cgctggatgt gtttagctct gaaccgttgg
     4261 cgcaatttag cccactgcta catgccaaaa atatcattac aacgccgcat attgcggccg
     4321 ccaccgttga gtcataccag caaaccggta cacacgttgc ccagtcgata attgattact
     4381 tcgcaggaag ggagataaaa aacgtgttgt gagttcagcg aacgaagttt ttaaatacaa
     4441 aaaaaaccgc aatttgtgga acacattgcg gtttatcagc attctaagta ttggttgcgg
     4501 gggccggatt tgaaccgacg accttcgggt tatgagcccg acgagctacc aggctgctcc
     4561 accccgcgtc cgtggatgcg cactatactc cgctcgcgtt ttcatgcaac ctttttttta
     4621 ccccgatcac ggatttagca tgattttgaa caaaaacaaa ccactaaagc ctattttttg
     4681 tgcaaaattt gcgtcaggga gaggctgctc atttcattaa acgcggcggt ttgttatctt
     4741 cgttgcgcct tattttttaa cctgaagaag agaacaatga aaggacgttg ggtaaagtac
     4801 cttcttatgg gcacggttgt ggcaatgctt gccgcctgct cttccaaacc aaccgatcgc
     4861 ggacagcaat ataaagacgg gaaatttacc cagcctttct ctctggtgaa ccagccagat
     4921 gccgttggcg cgccgattaa cgccggtgat tttgccgagc aaattaacca tatccgtaat
     4981 tcgtcaccgc gtctgtatgg caaccagagt aatgtttata acgcggtgca agagtggctg
     5041 cgcgcaggcg gtgatacccg caatatgcgc cagttcggca ttgatgcctg gcagatggaa
     5101 ggtgccgaca actatggtaa cgtgcagttt acaggctatt acacgccggt aattcaggcg
     5161 cgccataccc gccagggcga gttccagtat cctatttacc gtatgccgcc aaaacgtggt
     5221 cgtctgccgt ctcgtgcgga gatctacgca ggggcgctga gtgataaata tattctcgct
     5281 tacagcaact ccctgatgga taacttcatt atggatgtgc agggtagcgg gtatatcgac
     5341 tttggtgatg gcagtccgct taactttttc agctatgcag ggaaaaacgg tcatgcctat
     5401 cgcagcattg gtaaggtgct gatcgaccgt ggcgaagtga aaaaagaaga tatgtcgatg
     5461 caggcgattc gtcattgggg cgaaacacac agtgaagccg aggttcgcga gctgctggaa
     5521 cagaacccgt ctttcgtttt ctttaaaccg caatcttttg caccggtgaa aggggcaagt
     5581 gcggtgccgc tggttggccg tgcttctgtt gcctccgatc gttcaattat tccgccaggt
     5641 actaccttgc tggcagaagt gccgctgctg gataataacg gcaaatttaa cggtcagtac
     5701 gaattgcgtc tgatggtggc gctggatgtc ggtggcgcaa tcaaaggcca acacttcgat
     5761 atctatcaag ggatcgggcc ggaagccgga catcgcgcag gttggtacaa ccactatgga
     5821 cgtgtctggg tgctgaaaac cgccccgggc gcaggtaacg tctttagcgg ctgatgtgtt
     5881 attctgtata ccgtttttac ggatacgaca gggtgaggga taacctcacc ctttttaatt
     5941 ctgaggtgct atgtctgtgg taattagtga tgcatggcgt cagcgttttg gtggcacagc
     6001 gcgtctgtat ggtgaaaaag cgttgcaact gtttgctgac gcgcatattt gtgtggttgg
     6061 tatcggtggt gtcggttcct gggcggcgga agcgctggcg cgcacgggga ttggcgcaat
     6121 cacgcttatc gatatggatg atgtgtgcgt caccaatacc aatcggcaaa ttcacgctct
     6181 gcgcgataac gtcgggctgg caaaagcgga agtcatggcg gagcgtattc gccagattaa
     6241 cccggagtgc cgtgtaacgg tggtggatga tttcgtgacg ccggataacg tagcgcagta
     6301 tatgagcgtg gggtattcgt acgtgattga tgccattgat agtgtacggc ccaaagcggc
     6361 gctgattgct tattgtcggc gcaataaaat cccgctggtc acgaccggtg gcgcgggtgg
     6421 gcagattgac ccgacgcaga ttcaggttac cgatctggcg aaaacgattc aggatccgct
     6481 ggcggcgaag ttgcgcgagc gcctgaaaag cgattttggc gtagtgaaaa acagtaaagg
     6541 taagctcggc gtcgattgcg tgttttctac tgaagcgctg gtgtacccgc agtcagacgg
     6601 tactgtgtgt gcgatgaaag ccacggcaga agggccgaag cggatggatt gtgcatctgg
     6661 atttggcgcg gcaacgatgg tgaccgccac ctttgggttt gttgcggttt ctcatgcgct
     6721 gaagaagatg atggcgaaag cggcgcgtca gggttaaaca atcaggtcgg atgcgacgcg
     6781 aacgtcttat ccgacctggt tctgtgctta aacctgcttc gtcgcagcga taatcgcctc
     6841 gcttaacgca ttcaacccct ggctgcgtga ggcgctaagc tgcccacgca atcccagctc
     6901 atcaaacaat gccagcggcg actgtgcctg caattcggcg gcggttttcc cctcaatggc
     6961 agtcaacagc accgccagca ggccgcgcac tatacgtcct tcgctgtcgc caaagaagtg
     7021 cattttaccg ttttcagcca cggtatatcc cagccagacg cggttttcgc atccggcaat
     7081 ctctttagcc tgcgctttta actcgtctgg caaagccgga agctgtttcc ccagcatgat
     7141 caactggcga tatttatctt cccattgcgt caacggtgcg aaggtattgc gtaacgtttc
     7201 tgcggttacg gttgtgccga acggatgtcc ggcgaattgc gggttcgtca ttaatccacc
     7261 aataattcca gcgcgcggtc aacggcattc accagcgcat ccacatcact ctttgtatta
     7321 tatggcgcaa aagaggcgcg cagtgtgccg gttacgccta attctgccag tagcggctga
     7381 gcgcaatgtt gcccggcccg caaggcaata ccgtactccg ccagcagcgt caccatatcg
     7441 ctgtgatgaa cgccagcaaa atcaaaggcc agcaggctgg aatcctggca gcggaatgaa
     7501 cggaaacctg gacgtttcgc cagcgcatct tccgccagtg ttgctaagct acggctccag
     7561 ttttcggcct ggttgatatc gtaatctgcc agccattcca gcgccgcgct taatcctatg
     7621 acaccagcca catttggcgt tcctgcttcc agtttccacg gcgcagattg agtcgtgaag
     7681 ccgtcaaaac tcacttcgtg aaccattttg ccgccgccca gccagggcga catcgcttcc
     7741 agcagttctg atttgccata cagcacgccg atacctgtcg ggccatacag tttgtgacct
     7801 gaaaaagcat agaaatcaat attcagttgc tgaacatccg cggggaaatg caccgccccc
     7861 tgagcaccat caaccatcac caccatcccg gctgaatgag caaaggtaat cgctcgcgcc
     7921 agatccgggc aaccgccagt aacgttcgac atctgaccca acgccagaat ccgactacgg
     7981 ggagtaatca gttctggcaa caaatcgaca tccggcagtc gctgcgcatt aagcggcaat
     8041 ttcaccactt tggctccagt ttgttgggcg accatcagcc aggggacgag gttggcgtgg
     8101 tgttctgcca cgctgacaat aatctcatcg cccagttgca gacgcggacg cgcatagcat
     8161 tgtgcaacca tgttgatgga ttcagtggtg ccgcgcgtcc agacgatagt tttatcatcc
     8221 ggtgcattca gtaattgcgc cactttctct cgtgcagctt cataacgcgc ggtcaggcgt
     8281 tgggcttcgg caaactggct gcgatggacg tttccggcgc tcaggctgta aaactgtcgg
     8341 gtggcttcaa ccacggcttc aggtttaagc gcggtcgcgg cgctgtcgag atagacgccc
     8401 gcatcctgta gagcgggaaa ctgggcgcga aactgcgcgg gattaaaaac gttcatggta
     8461 ctcctcggct tgatgcaccg atcgtgacgc aaaattcact ttgatacaag gagagtgcta
     8521 accatcagaa ttatctggat atcctggcga agccgcgtaa gtaacatatg ctgaatagtg
     8581 ttaggcgtta attttagcct gttaatcaat actattaaga tttattaatc gctttaagga
     8641 gaagatgatg aaaaagactg ccgcaattat ttctgcctgt atgctgactt ttgccctgag
     8701 cgcctgttcc ggttcgaact atgtgatgca caccaatgac ggacgtacca tcgtctctga
     8761 cggcaaacca cagactgata acgataccgg tatgatttcg tataaagacg ctaatggcaa
     8821 caaacagcag atcaaccgta ctgacgtgaa agagatggtc gaactggatc agtaattcac
     8881 gatcgcaagg taaaaaaaag caccgcaatt aggcggtgct acattaatca ctatggacag
     8941 acagggtaaa tgtacaggaa gtgaaaaaag gtagctttgc taccatggtc tgaatcgcag
     9001 accaattgca aacacaacaa cacaacatca caaccgtaag ccaaaagttc accagaacac
     9061 gcattccgat aaaacttttc gttccggctc aggaagtgcc gccactatag gtatttgctg
     9121 gtagaagctc aacggacaat ttataatggc tcagattaaa aaaactaata ggttacacag
     9181 tgtgatctaa ttgttaaatt catttaacat caaagtttaa aagccatgtc taaacgatta
     9241 ccaccgctaa atgccttacg agtttttgat gccgcagcac gccatttaag tttcactcgc
     9301 gcagcagaag agctttttgt gacccaggcc gcagtaagtc atcaaatcaa gtctcttgag
     9361 gattttttgg ggctaaaact gttccgccgc cgtaatcgtt cactcctgct gaccgaggaa
     9421 gggcaaagct atttcctcga tatcaaagag atattttcgc aattaaccga agcgacgcgt
     9481 aaactccagg cccgtagcgc caagggggcg ttgacggtca gtttactccc cagtttcgcc
     9541 attcattggt tggttccgcg actttccagc tttaattcag cttatccggg aattgacgtt
     9601 cgaatccagg cggttgatcg tcaggaagat aagctggcgg atgatgttga tgtggcgata
     9661 ttttatggtc ggggcaactg gccggggcta cgggtggaaa aactgtacgc cgaatattta
     9721 ttgccggtgt gttcgccgct actgctgaca ggcgaaaaac ccttgaagac cccggaagat
     9781 ctggctaaac atacgttatt acatgatgcg tcacgccgtg actggcagac atatacccga
     9841 cagttggggt taaatcatat caacgttcag caagggccaa tttttagtca tagcgccatg
     9901 gtgctgcaag cggctattca cgggcaggga gtggcgctgg caaataacgt gatggcgcaa
     9961 tctgaaattg aagccggacg tcttgtttgc ccgtttaatg atgttctggt cagtaaaaac
    10021 gctttttatc tggtttgtca tgacagccag gcagaactgg gtaaaatagc cgcctttcgc
    10081 caatggatcc tggcgaaagc cgctgctgaa caagaaaaat tccgctttcg ttatgaacaa
    10141 taatttacgt agggtacgac catgaccagc cgttttatgc tgattttcgc cgccattagc
    10201 ggcttcattt ttgtggctct gggcgctttt ggcgcgcatg tgttaagtaa aaccatgggg
    10261 gccgttgaga tgggctggat ccagaccggc ctcgaatacc aggcgtttca tacgctggcg
    10321 atcttaggtc tggcggtggc aatgcagcgt cgcatcagta tctggtttta ctggagtagc
    10381 gttttcctcg cgttaggcac ggtgctgttc agcggcagcc tttattgcct ggcactgtcc
    10441 catctgcgtt tgtgggcgtt tgtcactccg gttggcggcg tgagcttcct tgcgggctgg
    10501 gcgttaatgt tagttggtgc tatccgttta aagcgcaagg gcgtaagtca tgaataaggt
    10561 tgtattgctg tgccgtccgg gctttgaaaa agagtgcgcc gcagaaatta ccgataaagc
    10621 cggccagcgg gaaattttcg gttttgcccg cgtgaaagag aatgcgggtt atgtcattta
    10681 tgaatgttat caacctgatg atggcgataa gttaatccgt gagctgccgt tcagttcatt
    10741 aatttttgcc cgccagtggt ttgtggtggg ggaactcctg cagcatttgc cgccagaaga
    10801 tcgtattacc cccattgtcg gcatgttaca gggcgtagta gagaagggcg gtgaactgcg
    10861 tgttgaagtt gccgatacca acgaaagcaa agagttgctg aaattctgcc gtaaattcac
    10921 cgtgccgcta cgcgctgcct tgcgcgatgc cggggtgctg gcgaactatg aaacgccgaa
    10981 gcgtccggtt gtgcatgtat tcttcattgc accaggttgc tgctataccg gttactcata
    11041 cagcaacaat aattcgccgt tctatatggg cattccgcgc ctgaaatttc cggcagatgc
    11101 gccgagtcgt tccacactca aactggaaga ggcatttcat gtgtttattc ctgcggatga
    11161 gtgggatgaa cgcctggcga acgggatgtg ggcggtggat ttgggcgctt gcccaggcgg
    11221 ctggacctac caactggtga agcgcaacat gtgggtttat tccgtcgaca acggcccgat
    11281 ggcgcaaagt ctgatggata ccgggcaggt gacgtggctg cgggaagacg gtttcaaatt
    11341 ccgtccgaca cgcagcaata tctcctggat ggtatgcgat atggttgaaa aaccggctaa
    11401 agttgcggca ttgatggcgc agtggctggt taatggctgg tgccgtgaaa ccattttcaa
    11461 tctcaaactg ccgatgaaaa aacgctacga agaagtatca cacaatctgg cgtatattca
    11521 ggcacagctt gatgaacatg gcataaatgc tcagattcag gcgcggcagt tgtatcacga
    11581 tcgcgaagaa gtgacggtgc acgtccgccg catctgggct gcggtgggtg gtcgtcgcga
    11641 cgagcgataa caggcaaggc cggggcgtcg tctccggcct gctacccttt ccgtcaggct
    11701 gttaccaaag aagttgcaac ctggcgctca tcagaaataa tgtgcgttac ctcatccaga
    11761 tgcccgatgc gggcttcccc tgaacgatta aatttacttt tatcaatcaa taacaacgat
    11821 tgcgcggcac gttttaatag catcgatttg taatcagcgt tgatggcatt ggagtcccac
    11881 agtgcgccgc tgctatcgat cccttcacaa gaaaaaataa acagatcgat ttccagcgat
    11941 ttaagttggg aaatcagcga gggattgacg taacagccat atttgcgctc aagtgtgccg
    12001 ccggaactga tcagttgaat gcgttcgcgt ttaccgagtt catggcaaat cggatggcta
    12061 ttggtgaaga cctgaatgtt gatgtcaggc aactggcgtg ccagatacca gcaagttgaa
    12121 ctggcgtcta aggctatcac catcccttct tcaatccacg cgagcgcctc gcgcgcgata
    12181 tctgctttat gcgcataatg gcttttcagc cggatgtgaa agggatcgcc actgtcttga
    12241 ttttgacggt ggatatattt agcgcgtcca tgattgcgca gaattttacc ctgcgtctgt
    12301 aattcattga gatcgcgacg aatggtttct ttactgacct ttagctgttc agagagagct
    12361 tccgtggtca ggctggtatg gttcagcagc aggtcgacta tcgcttgctg gcgtgccgct
    12421 ttcatatttt tcaccccatc attcgataac cgcccgcgtc atgcgggcgt accggtatga
    12481 gattacggtg ttaccccttt ttttaaaaga atattcccgt acttcgctcg ttcgcctgtg
    12541 ataacgatcg caaaggcttt ttgcgcccgt tcataaaacg caaaacgatt gatgcggatg
    12601 atgtcaggac acggggcttg tagtgaaagc gcattacggt aacgtcgttc tacttcagga
    12661 tcgagagtgt caccttctac cgctgccatc atcaccagcg gcggggcata actgtccagc
    12721 tcaaataacg ggataatcgc ctggagcaag tcgctcacca acaggccatc agcgcggatc
    12781 acctgcggtc ccatcgaatg ggcgggaaag tgagcatcgg aaaaaataat ttcatctcca
    12841 tgtcccatct ctgccagcac tttcaatagt tcgggagaaa ttaacggcga aattgttttc
    12901 agcatttcac acttcctcta taaattcagg ttcagtttgc gggtagaaat aacggtactg
    12961 ataatgaatc tgtgcacggg cttcttccgg gctgttgaat tcccctacgc cataccaacc
    13021 gaacagcgca gctcctgcga cggtcgtttc ggcatcgtcg agaactttta ccggaatatc
    13081 aagcatattg gctttaatct gattccacaa tgtgttgcga cttcctccac cgactaacaa
    13141 taattcagag gccttaaagt gcccgatttt ttccagcatc tgtagattgc gctgtaattg
    13201 cgcagttaac ccttccagcg ccgcgcgata gaaatgcccc cgcgtggtat taagcgtcac
    13261 tccttgccag ccagcgttct gacacgacaa taaatcacac tgcattttta cgccatccgc
    13321 gccaggcgcg atcagacgag cttcttcaat caacatttgc cagggtgttt cagccgtcca
    13381 gaacagtttt ctcacccatt ccagcacgcc ggatgccagc cattgcatac ctgggttata
    13441 caaccctgcc tggctatcca gttcgcaggt ggaaccggcg tactgactta acagcgaagt
    13501 atcaacctgg gcgctacgga ccattaaaat ttcccatgta ccggaagaga gcacgggttc
    13561 attttggtcg gcaccagcgc caaaaagggc gaactgggta tcgtgacctg cggaaatcac
    13621 cggtatgcca acgggtaagc cgagcattgc tgcggcgctg ttctgtagcg taccaatctg
    13681 ttcacccgct tccaccagac gagggaagag tcggcgtgga ataccggtgg cttgtaaaat
    13741 ttgcggactg aaatcgcgtt gctggatatc cagcatctgg ctggttccgg ccatcgtgat
    13801 atcagtagtg aattcgccgg ttaaacggtg gttaatcagc gacgaaataa agagccaggc
    13861 gtgcgcgcgt tccagcagtt gtggatgatt ttctttcaac cacaccaact tatataacgt
    13921 attgaaacta aaggctccga cgccagaaat agcctgcaac cgctgtgcgg agatcaactg
    13981 ttcaatattg tccataaccg ctgctgttcg cggacatttc cagctaatga tcggatagag
    14041 cagattgcct tgcttatcta ccagagcgcc atccacacca aaggtggtga cggcgatacc
    14101 gcggatgtgg cattcagtca gttcactatt gatttgccga cagcaatcag caaagcgttg
    14161 caaaatggcg tctaaagacc actggtgcca ggtgttgttt tccatcgcga tatcgctggc
    14221 attaggcgtt gaggcgcggg caacaatttt tccctgccga ttaaccgcga tggccctgac
    14281 attggtcgcg ccacagtcga gtaccaggat aacttcttgt ttcataatcg ctcctgcaat
    14341 atagccggat aacattgctt atccggctaa ccactcttgc ttgttcaggc ccggtagccg
    14401 gagcgaccgg gcatcacatc agggagtaat gtattaacgc ttgtacaacg ggccgtagtt
    14461 ctggcaagcg cggtaatcct ggccttcaat atccatgccg tgcgcagccc aggcagaagg
    14521 acgatacact ttggtctctt caacgttgtg catacatacc gggatacgca gcatggaggc
    14581 gagagtgata aagtctgcgc caacgtggcc gatggtcaga accccatggt tagcgcccca
    14641 gttcgccatt accgagtaca catccgtaaa cggcccttta ccggtgaggc gcggtgcaaa
    14701 ccaggtggtt ggccaggttg agttggtgcg tttgttgagg atgtcatgca catccttcgg
    14761 caattccacg ctccagcctt ccgcgatttg cagtaccggt cccaggcctt tgatgatgtt
    14821 gacacgggtc atggtgaacg ggacgccgcc ttcggtaagg aagcgggaag agtaaccgcc
    14881 gccacggaag tattcgtgga tcgccgggca ccattcggta gcggcgaggc aagcgtcagc
    14941 ctcttgctga gagatttccc agtgcggctt catcgtcggg ttaccttcgc tgtcgcgttg
    15001 tttacaggaa ccgtccagcg cagcagaacc ggagttgatc aaatggatga tgccgtgttc
    15061 tgccagtcca tcgagtttat gccccgttac acgctcaatt gcttctggtg accagtaggt
    15121 acgcacatcg gcaaatacct gagcggtgcc ggtgagctgg tgacccatta gcattgccac
    15181 gccgttaaga ctgtcgtttt cggtcgccac gacaaagggt tcgcgcacgc cattccagtc
    15241 aaatgaactg ttaaggatcg cttcggcggt gtcaccattg ggatattgat cggtccagtg
    15301 acgttgcccc tggaagcccg cagcgatggc gttgtagcca agtgattctt ccacgcgacc
    15361 aatatcggcc agtttgctgt tgccttgcat catgtcgcgg atacacatcg ccatcagtaa
    15421 actttcgcgc agaactgcgc ggcgttgctc ggcattgcgt tgatactgtt tgttattttc
    15481 atcttcgcca tagcggaagt ttttatcagc ccaggccagt gccatttcca gttcggcttc
    15541 gtcgtaaatc ttctgatcga tacggcgacg cagttcggtc atatccaccg cctggacttt
    15601 cattcccagc caggattcaa agaagttgtg atcaacaatg gaaccggcga tacccatcga
    15661 aacgccgccc agcgacagat agcttttacc tttcatgctg gcgacggcca aaccggcgcg
    15721 ggcaaagcgc agcagttttt cttcaacatc ggcaggaatc gatgtgtcat cggcatcctg
    15781 aacgtcatga ccgtaaatgg agaatgccgg gatgcctttc tggctgtgag ctgccagagc
    15841 cgctgccagg taaacagcgc cgggacgttc agtgccgtta aagccccaaa tggctttcgg
    15901 gcgggttgga tccatgtcga tggtttcact gccatagcac cagcaaggcg ttaccgtaat
    15961 ggtgaggcct acattctgac tgctgaattt ttcttcgcaa gcagcggctt cagccatacc
    16021 cgcgatacag gtatcggaaa tgacacactc gacggcagct ccgcaggcat ggcgcagttt
    16081 ctcggtcagc agtgcggccg tagctttcgc catattcatt gtttgttctt caagcgactc
    16141 acgaacaccc atgcgacgac cgtcaataac cgggcggata ccaattttcg gtaagctgat
    16201 ttttttcatt acagattcct cactttattc ggaaaatatg ttcagttagt tgccgtttga
    16261 gagcggaaac gggcaaagat aaagatgacc gcgaagcaga gtgcggggat cagttcagca
    16321 gtggggatgt tgcccgccgc gtcactgaca aaacccatga ccggagtgac aataccgccg
    16381 ccaatgatgg tcataacgat gaaggacgaa ccgtatttgg tgtcctggcc gagattctta
    16441 atgcccagcg agaagattgt tgggtactga atcgacataa aggcgctgca taaagtcagg
    16501 gctattaagc ccacatgacc gccagcgaag gctgagatca ggcacagtgc catagcgatt
    16561 aatgcgtagg cggccaggac tttgtgtggt gcgaagcgac tgatgagcca ggtaccggtg
    16621 aaacgaccaa taaagaagca caccatggtt ccggttaaat agttagcggc aaagcctgca
    16681 gtcatacctg gaatttcttc tacagcgtag cgaatcaaat agctccagca ggccgtttgt
    16741 gcgccaacgt agcagaattg cgctaatact gcccagcgcc agtggcgaat acgcgccagg
    16801 cgagaaagcg atgcggagaa cgatccttgt ttggcgtcac tgtgattatc actctgcaat
    16861 gccgggaatt tcgtcagcat gatcagcagg gcgaccagta acacgatagc cacgatgatc
    16921 atataaggtg tctgtaccga taataccagg ctgtgtttat acgcactcaa ttgctctgga
    16981 gacattttat cgagaacgtc ttgcgattga tgtggcacgt tagacaaaat aagactttgc
    17041 ccaaagacaa ccgcgataat tgcgccaaac gagttaaatg tttgcgcaag atttaagcgg
    17101 aagtgaccac tgctttccgg ccctaatacc gtaacaaaag ggtttgcggc agtttccaga
    17161 caacctaatc cggctgcaat aataaatagg ccaactaaaa ataaggtgta gttcattatt
    17221 tctgcagcgg gccagaataa tgcagcgccc aaggcatata aaaataatcc ggtaataatc
    17281 cctgctttat aactgagttt tttcatcaat atcccagcag ggattgggat aatgaaataa
    17341 ccaaagtaaa aggccgattg gatcaggcca gcctggaaat ttgtcagcgt aaaagcctgc
    17401 tggaattgag gtaataaaat gtcgttaagg ttattggcta ccgcccaaag aaaaaacagt
    17461 gagcacagca gcgcgaatgg aataatgtaa cttctgcttt gccctgcatc tttatctacc
    17521 gcacggtaac tctgcgtttg tattgatgtg tttcccatag catcctctta ggttcagaag
    17581 cttaagcacg taatcggtta tgttttcaaa agtgcaaaac cagcgagttc gggcttcata
    17641 tcagtgaata tttgcctgtc tgaaatgaac tcatggattc attttcaaat aaaaaacgct
    17701 ctgtaatatg acggcggtca cacttattag ctggaaggtg actttatgtg actaccatca
    17761 ctttaataat cgaaatttca tgcccgtttc agaaaataaa tcataaaaaa cacaaaccag
    17821 atgaccgtat taattgttta tgccgaaaac gggcattcct ggcttgagtg aatagaaaaa
    17881 tgaccgtttt gataaatatt tttaagagcg gtcattttta atggggttat aaaaagagaa
    17941 cttgcagtta tttactgtga ttagttgaac caggtcacaa aatataagct tgtgaatttc
    18001 tttctggtta atcaggaaga agatggtggg taattaaacg gctaattcaa tagtgtgaaa
    18061 ggaacaacat tattgccctg ttttgaatca gagagaggta gctaatggaa cgaaataaac
    18121 ttgctcgtca gattattgac acttgcctgg aaatgacccg cctgggactg aaccagggga
    18181 cagcggggaa cgtcagtgta cgttatcagg atgggatgct gattacgcct acaggcattc
    18241 catatgaaaa actgacggag tcgcatattg tctttattga tggcaacggt aaacatgagg
    18301 aaggaaagct cccctcaagc gaatggcgtt tccatatggc ggcctatcaa agcagaccgg
    18361 atgccaacgc ggttgttcac aatcatgccg ttcattgcac ggcagtttcc attcttaacc
    18421 gatcgatccc cgctattcac tacatgattg cggcggctgg cggtaattct attccttgcg
    18481 cgccttatgc gacctttgga acacgtgaac tttctgaaca tgttgcgcag gctctcaaaa
    18541 atcgtaaggc aactttgtta caacatcatg ggcttatcgc ttgtgaggtg aatctggaaa
    18601 aagcgttatg gctggcgcat gaagttgaag tgctggcgca actttacctg acgaccctgg
    18661 cgattacgga cccggtgcca gtgctgagcg atgaagagat tgccgtagtg ctggagaaat
    18721 tcaaaaccta tgggttacga attgaagagt aatttcgtaa agcaacaagg agaaggatga
    18781 tggctaacag aatgattctg aacgaaacgg catggtttgg tcggggtgct gttggggctt
    18841 taaccgatga ggtgaaacgc cgtggttatc agaaggcgct gatcgtcacc gataaaacgc
    18901 tggtgcaatg cggcgtggtg gcgaaagtga ccgataagat ggatgctgca gggctggcat
    18961 gggcgattta cgacggcgta gtgcccaacc caacaattac tgtcgtcaaa gaagggctcg
    19021 gtgtattcca gaatagcggc gcggattacc tgatcgctat tggtggtggt tctccacagg
    19081 atacttgtaa agcgattggc attatcagca acaacccgga gtttgccgat gtgcgtagcc
    19141 tggaaggact ttccccgacc aataaaccca gtgtaccgat tctggcaatc cccaccacag
    19201 caggcactgc ggcagaagtg accattaact acgtgatcac tgacgaagaa aaacggcgca
    19261 agtttgtttg cgttgatccg catgatatcc cgcaggtggc gtttattgac gctgacatga
    19321 tggatggtat gcctccagcg ctgaaagctg cgacgggtgt cgatgcgctc actcatgcta
    19381 ttgaggggta tattacccgt ggcgcgtggg cgctaaccga tgcactgcac attaaagcga
    19441 ttgaaatcat tgctggggcg ctgcgaggat cggttgctgg tgataaggat gccggagaag
    19501 aaatggcgct cgggcagtat gttgcgggta tgggcttctc gaatgttggg ttagggttgg
    19561 tgcatggtat ggcgcatcca ctgggcgcgt tttataacac tccacacggt gttgcaaacg
    19621 ccatcctgct accgcatgtc atgcgctata acgctgactt taccggtgag aagtaccgcg
    19681 atatcgcgcg cgttatgggc gtgaaagtgg aaggtatgag cctggaagag gcgcgtaatg
    19741 ccgctgttga agcggtgttt gctctcaacc gtgatgtcgg tattccgcca catttgcgtg
    19801 atgttggggt acgcaaggaa gacattccgg cactggcgca ggcggcactg gatgatgttt
    19861 gtaccggtgg caacccgcgt gaagcaacgc ttgaggatat tgtagagctt taccataccg
    19921 cctggtaaat gcgctgatgt gataatgccg gatacgacgt ttgcggcgta tccggctcgc
    19981 cgttaccgta ccaaccgcaa ttgctgtaaa ttgccgtcga tatgcaaatc ggtttgtaag
    20041 cgggcaatat cgcggcacag aaacgccatc tctttatggg tttctaattt tttgcgccac
    20101 ttttcggcaa ccgcatccag attctcatat atcccttcca gactctgaaa ctcgaccagc
    20161 agctgcgtgg cgctttttgg tccgattccc gcaacacccg gtacctttga actgctgatc
    20221 cccgcaagtc cccagtaatc gggcaactgc tgcggttgaa cgccaaattc tttatcgata
    20281 aatggcgcat ccagccaacg tttctggaag taatcacgaa tacgtaatgt cggtgaaagt
    20341 aactgacagt agcctttatc tgtcgaaaca atcgttgcct gatgcccggc ctgtgtcact
    20401 ttgaccgcca gcgtggcggc taagtcatcg gcttcgttgc cgctggttga ccagcacggg
    20461 acgccgcgtt gctcaaaggc ggcgcgtaat gcaggcatct cgtcgtgcaa ctcttccggc
    20521 attggcggtc gacccgcttt gtaatctggt aaacgctgat gacgccagcc gctactgcgg
    20581 ttttcatcat caaaaacggc gaccgcgtgg gttggctggc tgtgcataat gagctgatcg
    20641 agcgcatgct ggcaggtttc gacacagggc gatccctgaa cggcatgaat gcgacgaata
    20701 agattcagtg catcgacaat aagcaaatga acagccacga taaccccctt aaagatcagg
    20761 cgataagggt aacattcgat gccggaacag gctacggctg gatgagaaat cgggaagagg
    20821 cctcgcaaaa cgaggcctct ggagagcgat taatcgcagg caacgatctt cattgccagg
    20881 ccgccgcgag aggtttcgcg gtacttggcg ttcatatctt tacctgtttc gtacatggtt
    20941 tcgataactt tatcgaggca gacgcgcggc tcgctggtac ggcgtagcgc catacgtgcg
    21001 gcgttcaccg cttttactgc cgcaatggcg ttacgctcga tgcatggcac ctggacctgt
    21061 ccggcgaccg ggtcacacgt cagaccgagg ttgtgctcca tggcgatttc cgccgcgatg
    21121 cacacctgcg ccgggcttgc acctaacagt tctgccagac cagccgccgc cattgagcac
    21181 gccacaccaa cttcaccctg gcagccgact tcagcaccag aaattgacgc gttcatctta
    21241 taaagagaac caatggcact ggctaccagc aggtaacgag ccagtgagtt agcgttcact
    21301 tcgcggataa acttgtcgta gtacgccagc actgccggga taatcccgca cgcaccgtta
    21361 gtcggcgcag tcaccacgcg tccgccagca gcgttctctt cgttcactgc caatgcaaac
    21421 atgttgatcc agtcaacaac cgccatcggg tcagtggtgg ttttatcctg gctgaccagc
    21481 atccggcgta gtgccgcagc acggcgtgga acgcgcagtt tgccaggcaa cacgccttcg
    21541 gtggaaatac cgcgctcaat accgccacgc atgacttccc atacgttcgc caggtgctgt
    21601 tccagctctt ctttgctgtg cagcgccagc tcatttttca tcatcaggcc agagagtgac
    21661 agcccggttt cctgacaatg tttttgcaga tcggctgctg aactgtacgg ataaggaact
    21721 tcaaccggtg cgctattctg ctggccaaaa tgctcttcat caacgataaa gccaccgcca
    21781 atagagtagt aagtctggct gtaaacgact ttatcgcccg ccagcgcggt aatgcgcata
    21841 ccgttttcat gcagagaaag gttgtctgca tggaagttca tgcactgatc aaccgggaac
    21901 tccacttcat gctgaccgtt tgccagcatc aggcgaccat gagtattcac atcctgaata
    21961 aaaccaggga tggaatcgat atccacggta tccggcaggt tacccgccag gcccataata
    22021 atggcgatat cagtgtggtg gcctttacca gtcagagaga gcgagccgta cacgtcaacc
    22081 accacgcggg tcacgtcttt aagcaggtta cgggcaatca gatcgtcggt aaattgttta
    22141 cccgctttca ttggtccaac ggtatgagaa ctggaagggc caatgccgat tttgaaaata
    22201 tcgaatacgc taatcatagg aaatacatcg cgttaaaacg gaggaagcgc cgcccgaaag
    22261 cggcgcgaaa ggacttagct gaacagagag tagaagattg cggagattgc aatcagaccc
    22321 atcacgacaa cgaatacgtt gctgatgtga ccgctgtact tacgcattgc cggtactttc
    22381 tgaattgcgt acatcggcat caggaacagg atcatcgcga tgattgggcc gcccagggtt
    22441 tcaatcatac ccaggatgct cgggttcagg gtggcaacaa tccaggtcgt taccagcatg
    22501 aacagcgcag tgatacggtt cagcttgttg atttcgatag acttaccttt accacgcaga
    22561 gatttaatca ccataccgtt gaagccttca cgtgcgccca ggtagtggcc gaggaaggat
    22621 ttggtgatag cgataatcgc gataatcgga gccatccacg cgataaccgg tgcgttaaag
    22681 tggttagcta ggtaagacag aatcgagatg ttctgctctt tagccgcagc caggtctgcc
    22741 ggagtcaggc tcagtacgca gctgaagacg aagaacatta cggtcagcac catcatgatg
    22801 tgtgcgaatg ccaggatctt cgagcatttc tgttctgcca tatcgccgta ctcttcacgc
    22861 ttcgcaacgg cgaaagaaga gatgatcgga gagtggttga acgagaacac cattaccgga
    22921 attgccagcc acagggtcat ccacagaccg tttccggttg cagatgcagt gtccagagac
    22981 agcgtttcca gtgctgcgcc gttccactgc gggatcaggt acagagccag cagcatcagt
    23041 acgccaacaa acgggaatac cagaatactc atcgctttaa cgatcatctg ctcaccaaag
    23101 cgaacgatgg tcatcatacc cacgatcagg atcagcgaca gaatcgcacg cggcggtggc
    23161 gtcataccca gctggtgaga catgaagctt tcaactgtat tggtgattgc cacgctataa
    23221 accagcagga tcgggtagat agcgaagaag tagagcaggg taatcagttt acctgcgcca
    23281 ataccaaagt gttcttctac aacctcggtg atgtcttcgc ccgggttttt accagacagt
    23341 acgaagcgag tcaggccgcg gtgagcaaaa aacgtcatcg ggaacgcaag gatagccatg
    23401 atgatcagcg ggatcatacc accaacaccg gcgttgattg gcaggaacag cacgcccgcg
    23461 ccgattgccg tgccgtaaag gcccagcatc cacatggtgt ctgtcttgcg ccaggcacta
    23521 cgagagtctt tcgacgcaat cgtgctggtt tgagtcgttt ccatctattt ctcctggagg
    23581 aatgcaaata attcagcttt tagccagttc agcccgggaa ggtgcctgac cgtaaaaatg
    23641 aaatctgtct atcggcgatg gcttaatcat ttaccgccgt aataatttga gggcggaaag
    23701 atacacacaa gttatgtatc tatcagtgat cttgatccca aatacaataa tctacttgga
    23761 agatagatta tcccaacgct ttactctgta aatcaaaagt aaaagagaat ttaagggcaa
    23821 tatttgccca ttaaacgaca tctgacttct tccggggcgc gcaatatatg taggttgaac
    23881 tgaaaaaatc aatactttta gttaatcatg gcgataattt ctttttcata gcgataaaat
    23941 acggtttatg aaaataggga aacgtttgcg ttgcaatata aattgtatga catattaact
    24001 gtgatgatat ttgtcgcgtg agggtggtaa gaaacattca tataacaaat gaaatgagtt
    24061 gatgagtaag tgaccaaaaa cgagaaaacc cggcaacaaa cgtcaccggg ggaggggaga
    24121 ttacgtgcag atttcgtagc aagggatgta ggctgagcct ggcaacttca tacgatgctg
    24181 ggcaacaaaa ccctgtagca ggtcgtccat acgacgcata atctctttat cgccgttgat
    24241 tttgtaagga ccaaactctt caatggcgcg aataccgact tcttttacgt tacccgccac
    24301 aataccggag aacgcacggc gcaggtcggc agccagcact tcaacaggtt gatccgggta
    24361 aagcttcaga ttagccatat tctcgtgaga cggctcaaac ggcatttgca aatctggtgc
    24421 aatgcgcatt gaccagttaa agctgtaagc gtcaccagtg tcgcggcgat tttctttcac
    24481 cagcggcatc gattttttca tctgacgagc gacttcagcg gcgtcatcaa tgatgatgcg
    24541 gtaatggcgg cgcgcgtttt cgcccagcgt atgtacgaca aactcgtcca gtacgcggaa
    24601 gtagtcggcg ctctctttcg ggccggtgag gatcaatggt aaaacctgat ctttgttggc
    24661 cgggttcatt aaaattccca gcaaatacag caactcttct gccgtaccca caccgccagg
    24721 gaagataatg ataccgtgag cgatacggac aaacgcttcc agacgttttt cgatatctgg
    24781 catgatgatc aattcgttga ccagcgggtt aggcggttca gcggcgataa tcgacggctc
    24841 ggtcatacca ataaaacgac tgtctttgta acgctgctgc gcgtgtccga ccgcagcacc
    24901 tttcatcggc gcttccatcg ctcccggacc acagccggtg cagatattca gctcacgcag
    24961 gcccagctgg tttccgacgc gacgggcata caaatactcg ttttcgttaa ttgagtgacc
    25021 gccccagcag accaccatat ttggcgcttc acccacatgc agcgcgcgag cgttacgcaa
    25081 gatggaaaag accaggttag tgatgtggac ggagttgtcg agattcaggt tgggaaaacg
    25141 aacggtatta tggatttgcc cgtaaacgaa gagaatgtct cgcagaaccg cgaacaagtt
    25201 ggcctgcaaa gcgcgaataa ttcgcccatc gacaaaagcc tcttccgggg gattaatcag
    25261 ttccagcttt acgccgcgtt cacggcgcag gacgttaata tcgaaatttt caaaacgaga
    25321 cagcaattct ttgctgttat cggtcaaact accggagttc agtacggcaa gtgaacagtt
    25381 gcgaaacagt tgatagaggt cgctgctggc ggtgcgttta agcatatcca cttccagctg
    25441 cgacaacata tccatggagc caagcgggct aatatgtgta atcaagaaaa actccttatg
    25501 ggacgaatat cgtcattccc tttgattaca atagccctgc ctgatgacct ttcacaacct
    25561 tctgcgtgaa tccagcacgc aaaattgaga aaaaatttat tgccgaacca gtcttgtggt
    25621 cgatgggaca aaatcgctat tactgcgcca cgggttaatg tccagaccgc cacgacgggt
    25681 ataacgtgcg taaacgctta atttctctgg ctggcagaag cgtaacaggt cattaaagat
    25741 gcgttccacg cactgttcgt ggaactcgtt gtgatgacgg aatgagacca ggtaacgcag
    25801 cagtttttct ctgtcaattt ggcgtccacg atactgaatt tggatcgaac cccaatctgg
    25861 ttgatgggtg atcaggcagt ttgatttcag caggtggctg acaagcgtct cttccactac
    25921 tttttcaccg ctggtggcat tctccagata gtcagtagtg aattcatagt tatcgatagt
    25981 gatatcctgg tcatcaatgc aggtgccatt gaaatgacct atcggctggc cttccagttc
    26041 atcaagacga tataacgcca cgctaacctt accctgggcg caagtgctta agtcgcgctc
    26101 cagcgtctgg cgcacctcat cccagttatt aaaacgcgtc tggttaaaac tgttgagata
    26161 gagcttaaaa ctcttcgact caatcagatt tgcgctggtg taatcaagtt caacgtgacc
    26221 gacggcaacc tgcggcaaac ctttcgcatt cagccaggaa agttcataca gcgtccagat
    26281 atccgtaccg tgaaaaggca ggttatccgc tttcagaccc agcgggtcgc gattcaggct
    26341 gcgtggaacg ccttgcagta ggttggcgtc ataggtatcc cggtaatcgg ttgattttcc
    26401 aagagtcagg cccgcaagtg cctgatggtt tgcataagaa gacatgtttc accgtcataa
    26461 accagataaa ctaaacgaca agtgtatctg gtttaagtga agagagaaat tagtggacga
    26521 tttgaccgca caagccctga aagattttac tgcacgctac tgcgatgcgt ggcatgaaga
    26581 gcataaaagc tggccgttaa gtgaggaact gtacggtgtt ccttcaccgt gcattatttc
    26641 aactaccgaa gatgccgtat actggcaacc ccagccgttt accggggaac aaaatgtaaa
    26701 cgcggttgaa cgcgcttttg atattgtgat acaacccaca attcatacct tttatacgac
    26761 tcagtttgcc ggggatatgc acgcgcagtt tggcgatatc aaactgacat tgctgcaaac
    26821 ctggagtgaa gacgacttcc gtcgggtgca ggaaaatctg attggtcatc tggtgactca
    26881 gaaaaggcta aaactgccgc ccacgctatt tatcgcgacg ctggaagaag agttagaggt
    26941 catctcggtg tgtaatctct ccggtgaagt gtgcaaagag acgctgggaa cacgtaaacg
    27001 gacgcattta gcctcaaatc ttgcggaatt ccttaatcaa cttaagcctc ttctgtaatc
    27061 caaatacccc atctggttgt gagagatctc ttacagactc tgtaggagat cgccaggaaa
    27121 taagcgaata cttaaaaaga taagaatcgt tattttcatt taaaatcaaa atgttgattg
    27181 ttaattctta actttcatat gaaattttcc ttaaggcatt gtctgtaagc gccttgtaag
    27241 acttcgcgaa aaagacgatt ctatcttcgt cgacagggag tcagacaacg aagtgaacat
    27301 caggatgatg acacttctgc aggacacacc aggatggtgt ttcagggaaa ggcttctgga
    27361 tgaagcgaag aggatgacgc aggacgcgtt aaaggacacc tccaggatgg agaatgagaa
    27421 ccggtcagga tgattcggtg ggtcaggaag gccagggaca cttcaggatg aagtatcaca
    27481 tcggggtggt gtgagcagga agcaatagtt caggatgaac gattggccgc aaggccagag
    27541 gaaaagttgt caaggatgag cagggagcaa caaaagtagc tggaatgctg cgaaacgaac
    27601 cgggagcgct gtgaatacag tgctcccttt ttttattcct gctatccttc gcggcagttt
    27661 ttcttattga ggttgcttta tgaccactca tgaccgcgtt cgtctccagt tgcaggcgct
    27721 tgaagcgtta ctgcgtgaac atcagcactg gcgaaacgat gaaccccagc cgcatcaatt
    27781 taatagtacc caaccgttct ttatggacac catggaacca ctggagtggt tgcagtgggt
    27841 gctgatcccg cgaatgcacg atttgttaga taacaaacag ccgttaccgg gcgcttttgc
    27901 cgttgcaccc tattatgaaa tggcgctggc gacggaccat ccgcaacgcg cgcttattct
    27961 ggctgagctg gaaaaactgg atgctctttt tgcggatgat gcgagctaat gctggaaata
    28021 ctctatcagg atgaatggct ggttgcggta aataaaccct ccggctggct ggttcaccgc
    28081 agctggctgg atcgcgacga gaaagtagtg gtcatgcaaa ccgtgcgtga tcagattggc
    28141 cagcacgttt ttaccgctca tcgtctggac cgacccactt ctggtgtgtt gttgatggga
    28201 ttatccagcg aggccggacg gctgctggca caacagtttg aacagcacca aatccagaaa
    28261 cgttaccatg cgattgtgcg cggctggttg atggaagaag cagttctgga ttatccactg
    28321 gtggaagaac tggacaaaat cgctgataaa tttgcccgcg aagataaagg ctcgcagcca
    28381 gcagtgacgc attatcgcgg tctggcgacc gtagaaatgc cggtagcgac cggacgttac
    28441 ccgaccacgc gctacggcct ggtggaactg gagccgaaaa ccggacgcaa acatcagctt
    28501 cgccgacatc ttgcccattt gcgtcatccg attattggcg atagcaaaca tggcgattta
    28561 cgccagaatc gcagcggtgc tgagcatttt ggcctccagc ggttaatgct acatgccagt
    28621 cagttgtcat tgacgcatcc ttttactggc gagccgctga ctattcacgc gggcctggac
    28681 gacacctgga tgcaggcgtt atcacaattt ggctggcgcg gtctgcttcc tgaaaatgaa
    28741 agggttgagt tcagtgcgcc atcgggtcag gatggagaga taagttccta atccagggag
    28801 aaaacggtat ggcggaaatt ggtatttttg tcggcaccat gtacggaaat tcgctgttag
    28861 tggcggaaga agcagaagca attctgaccg cacagggcca caaagcaacg gtatttgaag
    28921 atcctgaatt aagcgactgg ctgccctatc aggataagta tgttctggtg gttacgtcca
    28981 cgaccgggca gggcgacctt cctgatagca ttgtgccgct ctttcaggga atcaaagata
    29041 gtctgggctt ccagccgaat ctgcgttatg gcgtgattgc gctcggcgac agcagttatg
    29101 taaatttctg caatggcggc aaacagttcg atgccttgtt gcaggaacag agcgctcagc
    29161 gggttggcga aatactgttg attgacgcca gtgagaaccc ggaaccggaa acggaatcaa
    29221 atccgtgggt cgaacagtgg ggcacgctgt tgtcctgata tgttcagcga gcggtaaatg
    29281 tcgttttagc ggtgctgaat cgaatctttt caggcaaatg ccagtaaaaa ctgcttcata
    29341 gcgcggattt ttactggcgt ttgcctggag tcaagcgatc catttcatac tcttctttat
    29401 ttcttcgttt ttacccttcc tttcttgttc ttgttttcat ttccgtgaag tggattccac
    29461 cgtccagggc taatgccaaa atcgggcctc attgaacgca ttaatgttgt gttgttgcac
    29521 ggtgagccgc tatggcgcgc tttttatact gctattgcca gatataaaca cgcgccgtat
    29581 tcggcgaacg acctataaaa acggcaaaaa acaccctacg tcacctctga tttcctggcg
    29641 atgtcgcagt ccagagtgag cgtggctaac gcgaattttc aggagtgcaa caatgagttc
    29701 tttaagtcag gctgcgagca gtgtggaaaa acgcacgaat gctcgttact ggatagtggt
    29761 gatgttgttt atcgtcacat ccttcaacta cggcgaccgc gctacgcttt ctattgctgg
    29821 ttcggaaatg gccaaagata tcggccttga tcccgtggga atgggctatg tgttctctgc
    29881 cttctcatgg gcttatgtta tcgggcagat ccctggtggt tggttgctgg atcgttttgg
    29941 ttcaaaacgc gtctacttct ggtcgatctt tatctggtcg atgtttacct tgctgcaagg
    30001 cttcgtcgat atctttagtg gattcggcat tatcgttgcc ctgtttacgc tgcgcttcct
    30061 ggtcgggctt gctgaagcgc catctttccc cggcaacagt cgcattgttg cggcctggtt
    30121 tccggcgcag gaaaggggaa cggcggtgtc gatttttaac tccgctcaat acttcgcaac
    30181 ggtgatcttc gcgccgatta tgggctggct gacgcatgaa gtgggctggt cacacgtctt
    30241 cttctttatg ggcggtctgg ggattgtcat cagctttatc tggttgaaag tcatccacga
    30301 gccaaatcaa catccggggg taaatcagaa agagctggag tacatcgccg cgggtggcgc
    30361 gctgatcaat atggatcagc aaaacaccaa agttaaagtg ccgttcagcg tgaagtgggg
    30421 gcagatcaaa caactgctcg ggtcacggat gatgatcggc gtttatatcg gtcagtactg
    30481 tatcaacgcc ctgacttact ttttcattac ctggtttccg gtttatctgg tgcaggcgcg
    30541 tgggatgtcg attctgaaag cgggctttgt ggcttccgtt ccggcggttt gcggttttat
    30601 cggcggtgtg ctgggtggga ttatttccga ctggctgatg cgccgcacgg gatcgctgaa
    30661 cattgcgcgt aaaacaccga tcgtaatggg catgttgctg tcgatggtga tggtgttctg
    30721 caactacgtc aacgttgagt ggatgatcat cggctttatg gcgctggcct tcttcggtaa
    30781 gggcatcggg gcgctgggtt gggcagtaat ggcagatacc gcgccaaaag agatcagcgg
    30841 tctttccggt ggcctgttca acatgttcgg taacatttct ggcatcgtca cgccaatcgc
    30901 aattggttat atcgttggca cgactggctc ctttaatggg gcgctgattt atgttggtgt
    30961 tcatgcctta atcgcggtac tgagctacct ggtgctggtg ggcgatatca agcgtatcga
    31021 gctgaaacct gtcgcggggc aataagatga cgacacaatc cagtcctgtt attactgata
    31081 tgaaagtcat tccggtcgcc gggcacgata gcatgttgct taatattggt ggggcacata
    31141 acgcatattt cacccgcaat attgtggtac tcaccgataa cgccgggcat accggcattg
    31201 gtgaagcgcc gggcggagag gtgatttatc aaacactggt cgatgctatt ccgatggttc
    31261 tgggccagga agttgcgcgc ctgaataaag tggttcagca ggtgcataaa ggtaatcagg
    31321 cagccgattt tgataccttc ggcaaaggtg cctggacttt tgaattgcgc gttaatgccg
    31381 tggcggcgct ggaagccgcc ttgcttgacc tgctaggtaa ggcgctgaat gttccggtct
    31441 gcgaactgtt ggggccaggc aagcaacgcg agactattac cgtcctcggt tatctgtttt
    31501 atatcggtga tcggaccaaa accgatcttc cttatctgga aaatacgccg ggcaaccatg
    31561 agtggtatca gttgcgccat cagaaagcga tgaacagcga agccgttgtg cgtctggcgg
    31621 aagcctcaca ggatcgctat ggctttaaag atttcaaact taagggcggg gtgttacctg
    31681 gcgagcaaga aatcgacact gttcgggcat tgaagaaacg cttccctgat gcgcggatta
    31741 ccgttgatcc caacggtgcc tggctacttg atgaagccat ttctctatgc aaagggctga
    31801 atgatgttct tacctatgcc gaagatccat gcggcgcaga acagggtttc tccggtcgtg
    31861 aagtgatggc ggagtttcgg cgggcgaccg gcttgcccgt cgcgactaac atgatcgcca
    31921 ccaactggcg cgaaatgggt catgcggtga tgctcaatgc ggtagatatt ccacttgccg
    31981 atccgcactt ctggacgctt tccggtgcag tccgtgtggc gcagctttgc gacgactggg
    32041 ggctgacctg gggctgccat tctaataacc atttcgatat ctctctggcg atgtttaccc
    32101 atgtgggcgc ggcggcaccg ggtaatccta ccgctatcga tacccactgg atttggcagg
    32161 agggcgattg tcgcctgacc aaaaatccgc tggagattaa aaacggaaaa attgccgttc
    32221 ctgatgcgcc cggtctgggc gtggaactgg actgggaaca ggtacaaaag gcacatgagg
    32281 cctataaacg tctgcctggc ggtgcgcgta acgacgcagg tccgatgcag tacctgatcc
    32341 ccggctggac ctttgaccgt aaacgtcccg ttttcggccg tcattgattc tgaaaaagga
    32401 cataaatatg agttctcaat ttacgacgcc tgttgttact gaaatgcagg tcatcccggt
    32461 ggcgggtcat gacagtatgc tgatgaatct gagtggtgca cacgcaccgt tctttacgcg
    32521 taatattgtg attatcaaag ataattctgg tcacactggc gtaggggaaa ttcccggcgg
    32581 cgagaaaatc cgtaaaacgc tggaagatgc gattccgctg gtggtaggta aaacgctggg
    32641 taaatacaaa aacgttctga cgctggtgcg taatactttt gccgatcgtg atgctggtgg
    32701 gcgcggtttg cagacatttg atttgcgtac cactattcat gtagttaccg ggatagaagc
    32761 ggcaatgctg gatctgctgg ggcagcatct gggggtaaac gtggcatcgc tgctgggcga
    32821 tggtcaacag cgtagcgaag tcgaaatgct cggttatctg ttcttcgtcg gtgatcgcaa
    32881 agccacaccg ctgccgtatc aaagccagcc ggatgactca tgcgactggt atcgcctgcg
    32941 tcatgaagaa gcgatgacgc cggatgcggt ggtgcgcctg gcggaagcgg cgtatgaaaa
    33001 atatggcttc aacgatttca aactgaaagg cggtgtactg gccggggaag aagaggccga
    33061 gtctattgtg gcactggcgc aacgcttccc gcaggcgcgt attacgctcg atcctaacgg
    33121 tgcctggtcg ctgaacgaag cgattaaaat tggtaaatac ctgaaagggt cgctggctta
    33181 tgcagaagat ccgtgtggtg cggagcaagg tttctccggg cgtgaagtga tggctgagtt
    33241 ccgtcgcgca actggcctgc cgaccgcaac caatatgatc gccaccgact ggcggcagat
    33301 gggccatacg ctctccctgc aatccgttga tatcccgctg gcggatccgc atttctggac
    33361 gatgcaaggt tcggtacgtg tggcgcaaat gtgccatgaa tttggcctga cctggggttc
    33421 acactcaaac aaccacttcg atatttccct ggcgatgttt acccatgttg ccgccgctgc
    33481 accgggtaaa attactgcta ttgatacgca ctggatctgg caggaaggta atcagcgcct
    33541 gaccaaagaa ccgtttgaga tcaaaggcgg tctggttcag gtgccggaaa aaccggggct
    33601 gggtgtagaa atcgatatgg atcaagtgat gaaagcccat gagctgtatc agaaacacgg
    33661 gcttggcgcg cgtgacgatg cgatgggaat gcagtatctg attcctggct ggacgttcga
    33721 taacaagcgc ccgtgcatgg tgcgttaaga cagaaaacca aaaccggctc tatctggagc
    33781 cggcttgaga ctgatgctaa acgcaaaata gcctgttgtg ctatgtatca ggtctacatc
    33841 attttttaat tcattgaatt taattggttt tgtaggccgg ataaggcgtt aacgccgcat
    33901 ccggcataaa cacaggcact ttgtcaccaa tctgaaacca gcgtcataaa aagccgattg
    33961 ctactcgaca agacgtccgt tacccgagaa ttttgctggc ttcgcgcgct acattatcca
    34021 tctcgtccaa cagttccagc agctccggct ccaaatcttc ttctttagta ccgctacgta
    34081 gctgttgttc gatcagttgg cagagattct tcatacgcgg cacaccgcta tagccgcaac
    34141 tgccatgcag tttatgaatc aaatcaacca ggccttccgg gttttctcca accagctgtt
    34201 cctcaacttt gttgcgaact tcaggcagga aatcgagtaa catttgcagc atatcgcgcg
    34261 ctaaatcggt ttttcctgct gcctggcgta gtgccagttg ccagtcgagg gtcgcattcg
    34321 ggttcaccac aatttcgttg acttcgggcg tcacgacgcg agaggaaata ccgctgccag
    34381 gcttgtagcg caacaacaaa ttatgcaatc gctcttcttc aatcggtttc gccagataat
    34441 cgctcatccc tgcgccaagc agcttctctt tttgcccggc cattgcatgc gccgttaccg
    34501 cgataaccgg cgtttgttgc tgatggggga gctggtggat gagctcgcag gcccgaatgc
    34561 catccatgtc aggcatttga atatccatta agatcaaatc gaacggcatc tgtttcgccc
    34621 gttcaaccgc ctgatgcccg ctatcgcaaa gttccacatg ttgcaccata tcttccagca
    34681 atgcgccgat aagtttcagg ttagcggggt tgtcatcaac tgccatgact gtcattgcca
    34741 gcttgctttc atcggttaca ggcaaaagcg tgttttgttt gtgatgacaa aattccgtca
    34801 gggcaggcaa caggcgcgta ggtgttaatg gtttcagcag acacgcgccg ataccatctt
    34861 gcttgagttt ttctgcattg acttgtgcat ggcaaggaag tgccagcatc aggaaatcgg
    34921 tcatcgatac cgctttcgct aatcgctcat gttgcattgt tagcggctcg cggaaggtca
    34981 ccgcgatgcc taacaacatc atgtcgtaat gcgcgggagg cagcgcggag aacgttgggc
    35041 tataaaccac ttccagcggc gtttcactta aaatatccag cgtgcattgc gctgctgcgg
    35101 agtttggttc gacataggcc aggcgcttac cggcgaggca ctgggtggat ggcccttcga
    35161 taataatgtt cgggttcaga tcgagattaa tgtggaacca gaaagttgaa ccgcgattcg
    35221 gctggctatg gaacgaaata tcgccgccca tttcattaac cagtttttgt gtaatcacca
    35281 gccccagacc ggtgccacca tgacggcggg aaatactggc atcagcctgt cggaaggcct
    35341 ggaataagcg cgattgatcg cgttcaggaa taccaatgcc ggtatcccga atctgcactt
    35401 caatctgcac tttggtatta ctcagcgcac gtttttctac cagaatatca atgttgccat
    35461 tctcggtgaa tttaattgca ttccccacca ggttagtgat gatttgctgt aatcgcagtg
    35521 ggtcgccgat cacgttatca ggcacgtcgc ttttaatatt gagcgtcagt tccaaccctt
    35581 tatcgtgaga agaatgtgcc agcagagtaa cgacttcatc cagcgtgctg cgtagtggga
    35641 atggaatact ttccagaatc agcttacctg cttccagttt cgagaagtcg agaacatcat
    35701 taataattgc cagcaaatta tttgccgaac gttcaatcgt attcaggtga tcgcgctgcg
    35761 ttggtgttaa ttctgttttc agcgtcaggc gggtaaagcc aataacacca ttcagtggtg
    35821 tacgcagctc gtgtgacata tttgccagaa actcggattt aatacgcgcc gcttcctggg
    35881 cgcgcttttt cgccagatct aactcaacgt tctgaatttc catctgctcc agcgtttcac
    35941 gcagatcgga cgtcgcctgg tcgatattgt gctgcatctc ttcgtgataa gcagccagcg
    36001 acattgccat cgagttgata ccgtttttca gcatatccag ctcgccgagc ataaatcctt
    36061 ccacccggct gtcgagttgc ccgcgacgga tgcggtcgac ggtattcacc atgttgcgaa
    36121 tcggaccggt tacatcgcgc attaagcgcc agccaaaaat aagcgcaata ccgatacaaa
    36181 acagcatcat cacgctggaa ataaagatct ctttatattg ctgcaagcga accgatttaa
    36241 gatccagctc cagcgcaata tatcccaaca tattttgact atttttggca tcgctactgg
    36301 gcgattcgtc gggggagtaa ctctcagaaa taatcggcgt gcggaggatc ataatatcgc
    36361 cgtcacgagt gacagtgagc tggcgaggaa acggcacgtt gctgccgagc tgcattgatg
    36421 agggatcaag atgaaaattg gaggtgacaa agagtcggtt attttcatca taaaccgaaa
    36481 tcgcgcgaac aatatcggaa tggcgacgat gcagtacgct tattaactga ccgatagatt
    36541 cgcgattttg caggctcatg ccatattcag tagaaactgc aagcggctca ataatgctgg
    36601 caccggcatc ttccagttga cgctgcaagt cgttatagcg atgcacgacg aaaaagatac
    36661 tcagcaataa accaataagg acggtcggtg ccaggatcag aatcatcatg cgtgcgcgca
    36721 ggctgtagtt ggtcatggag ttccgttatg ggacaattaa ggtcacactg ttaaattgag
    36781 aaagtctcgc caatggcgca attctactct gcaaaacgac gcacgacgac gcgtcagatc
    36841 ataaccgttt cagtcaacga cctcgactct tttggtcagg gcgtggcgcg acataacggc
    36901 aaaacgttat ttatccccgg attattgccg caggaaaacg cggaagttgc tgttactgaa
    36961 gataaaaaac agtatgcccg cgctaaagtc gtacgccggt taagcgatag cccggaacgc
    37021 gaaacaccac gctgtcctca ttttggcgta tgcggcggct gtcagcaaca acacgccagc
    37081 gtggatttac agcagcgaag caaaagtgcg gcactcgccc gattaatgaa acacgatgtc
    37141 tctgaagtga tcgccgatgt tccctggggc tatcgccgcc gcgcgcgttt aagtctgaac
    37201 tacttaccga aaacacagca acttcagatg gggtttcgca aagcgggctc cagtgacatt
    37261 gtcgacgtta aacaatgccc cattttagtg ccccaacttg aagcattgct gcccaaagtc
    37321 agggcatgcc tgggcagctt acaagttatg cgccatcttg gtcatgttga actggtacag
    37381 gcaaccagcg gcacgctgat gattttgcgc cataccgcac cgctaagttc ggcagatcgc
    37441 gaaaaactgg aacgcttttc gcattctgaa ggcctggatc tgtatctcgc ccccgatagt
    37501 gagatactcg aaaccgtctc tggtgagatg ccctggtatg actcaaacgg gttgcgctta
    37561 acttttagcc cgcgcgattt tattcaggtc aatgcgggtg tgaaccaaaa aatggtagcg
    37621 cgtgcgttgg aatggctgga tgtgcaacct gaagatcgcg tactggatct gttctgcggt
    37681 atgggcaact ttacactgcc attggcgaaa caagcagcca gtgtggtcgg tgtagaaggt
    37741 gttccggcgc tggtggaaaa aggccagcag aatgcgcgtc ttaatggttt acagaatgtg
    37801 acgttttatc acgaaaatct tgaagaagat gtcacaaagc agccgtgggc gaaaaacggc
    37861 ttcgataaag tgttgctgga cccggcgcga gcaggtgccg caggtgttat gcagcaaatt
    37921 ataaaactgg aacctattcg tatagtttat gtatcctgta accctgcaac gctggctcgg
    37981 gatagcgaag cgttattaaa agcaggatat accattgcgc gactggcgat gctggatatg
    38041 ttcccacaca cgggacatct ggaatcgatg gtacttttct cgcgcgttaa atagttacga
    38101 tttgccgatt tcggcaggtc tggtccctaa aggagaggac aatggttgcg gtaagaagtg
    38161 cacatatcaa taaggctggt gaatttgatc cggaaaaatg gatcgcaagt ctgggtatta
    38221 ccagccagaa gtcgtgtgag tgcttagccg aaacctgggc gtattgtctg caacagacgc
    38281 aggggcatcc ggatgccagt ctgttattgt ggcgtggtgt tgagatggtg gagatcctct
    38341 cgacattaag tatggacatt gacacgctgc gggcggcgct gctgttccct ctggctgatg
    38401 ccaacgtagt cagcgaagat gtgctgcgtg agagcgtcgg taagtcggtc gttaacctta
    38461 ttcacggcgt gcgtgatatg gcggcgatcc gccagctgaa agcgacgcac actgattctg
    38521 tttcctccga acaggtcgat aacgttcgcc ggatgttatt ggcgatggtc gatgattttc
    38581 gctgcgtggt catcaaactg gcggagcgta ttgctcatct gcgcgaagta aaagatgcgc
    38641 cggaagatga acgcgtactg gcggcaaaag agtgcaccaa tatctacgcg ccgttggcga
    38701 accgtcttgg gattgggcaa ctgaaatggg agctggaaga ttactgcttc cgttatctcc
    38761 acccgaccga atacaaacgc atcgcaaaac tgctgcatga acgccgtctc gaccgcgaac
    38821 actatatcga agagtttgtc ggccatctgc gcgctgagat gaaagctgaa ggcgttaaag
    38881 ctgaagtgta tggtcgtccg aaacacatct acagtatctg gcgcaaaatg cagaaaaaga
    38941 acctcgcctt cgatgagctg tttgatgtgc gtgcggtacg tattgtcgcc gagcgtttac
    39001 aggattgtta tgccgcactg gggatagtgc acactcacta tcgccacctg ccggatgagt
    39061 ttgacgatta tgtcgctaac ccgaaaccaa acggttatca gtctattcat accgtggttc
    39121 tggggccagg tggcaaaacc gttgagatcc aaatccgcac caaacagatg catgaagatg
    39181 cagagttggg tgttgctgcg cactggaaat ataaagaggg cgcggcagct ggcggcgcac
    39241 gttcgggaca tgaagaccgg attgcctggc tgcgtaaact gattgcgtgg caggaagaga
    39301 tggctgattc cggcgaaatg ctcgacgaag tacgcagcca ggtctttgac gaccgggtgt
    39361 atgtctttac gccgaaaggt gatgtcgttg atttgcctgc gggatcaacg ccgctggact
    39421 tcgcttacca catccacagt gatgtcggac accgctgcat cggggcaaaa attggcgggc
    39481 gcattgtgcc gttcacctac cagctgcaga tgggcgacca gattgaaatt atcacccaga
    39541 aacagccgaa ccccagccgt gactggttaa acccaaacct cggttacgtc acaaccagcc
    39601 gtgggcgttc gaaaattcac gcctggttcc gtaaacagga ccgtgacaaa aacattctgg
    39661 ctgggcggca aatccttgac gacgagctgg aacatctggg gatcagcctg aaagaagcag
    39721 aaaaacatct gctgccgcgt tacaacttta atgatgtcga cgagttgctg gcggcgattg
    39781 gtggcgggga tatccgtctc aatcagatgg tgaacttcct gcaatcgcaa tttaataagc
    39841 cgagtgccga agagcaggac gccgccgcgc tgaagcaact tcagcaaaaa agctacacgc
    39901 cgcaaaaccg cagtaaagat aacggtcgcg tggtggtcga aggtgttggc aacctgatgc
    39961 accacatcgc gcgctgctgc cagccgattc ctggagatga gattgtcggc ttcattaccc
    40021 aggggcgcgg tatttcagta caccgcgccg attgcgaaca actggcggaa ctgcgctccc
    40081 atgcgccaga acgcattgtt gacgcggtat ggggtgagag ctactccgcc ggatattcgc
    40141 tggtggtccg cgtggtggct aatgatcgta gtgggttgtt acgtgatatc acgaccattc
    40201 tcgccaacga gaaggtgaac gtgcttggcg ttgccagccg tagcgacacc aaacagcaac
    40261 tggcgaccat cgacatgacc attgagattt acaacctgca agtgctgggg cgcgtgctgg
    40321 gtaaactcaa ccaggtgccg gatgttatcg acgcgcgtcg gttgcacggg agttaggccg
    40381 aaatttgctc gtatctacaa tgtagattga tatatactgt atctacatat gatagcggtt
    40441 tgaggaaagg gttatgatcc acagtagcgt aaagcgttgg ggaaattcac cggcggtgcg
    40501 gatcccggct acgttaatgc aggcgctcaa tctgaatatt gatgatgaag tgaagattga
    40561 cctggtggat ggcaaattaa ttattgagcc agtgcgtaaa gagcccgtat ttacgcttgc
    40621 tgaactggtc aacgacatca cgccggaaaa cctccacgag aatatcgact ggggagagcc
    40681 gaaagataag gaagtctggt aatggtaagc cgatacgtac ccgatatggg cgatctgatt
    40741 tgggttgatt ttgacccgac aaaaggtagc gagcaagctg gacatcgtcc agctgttgtc
    40801 ctgagtcctt tcatgtacaa caacaaaaca ggtatgtgtc tgtgtgttcc ttatacaacg
    40861 caatcaaaag gatatccgtt cgaagttgtt ttatccggtc aggaacgtga tggcgtagcg
    40921 ttagctgatc aggtaaaaag tatcgcctgg cgggcaagag gagcaacgaa gaaaggaaca
    40981 gttgccccag aggaattaca actcattaaa gccaaaatta acgtactgat tgggtagtgt
    41041 tactaactca cttctattct ggtcacaggt ttccacctga cagacccgca attttcagga
    41101 caattcaatg aatcaaatcg accgtttgct cactattatg cagcgcctgc gcgatccgga
    41161 aaacggctgc ccgtgggata aagagcagac atttgccacc attgcgcctt acacccttga
    41221 agaaacctac gaagtgctgg acgccatcgc ccgtgaagat tttgacgatc ttcgcggtga
    41281 actgggcgat ctgctattcc aggtggtgtt ttacgcgcaa atggctcagg aagaagggcg
    41341 ctttgacttt aatgatattt gcgctgctat tagcgataaa ttagagcgtc gccatccgca
    41401 tgtttttgct gatagttctg ccgaaaacag tagtgaagtg cttgcccgtt gggagcaaat
    41461 caaaaccgaa gagcgcgcgc agaaagcgca gcattcggcg ctggacgata ttcctcgtag
    41521 tttaccggct ttaatgcgtg cgcaaaaaat ccagaaacgt tgcgccaacg ttggcttcga
    41581 ttggacgacg cttggtccgg tagtcgataa agtctacgaa gagatcgacg aggtgatgta
    41641 cgaagcgcgg caggctgttg tcgaccaggc taaactggag gaggaaatgg gggacctgct
    41701 gtttgccacg gtcaatctgg ctcgtcattt agggacaaaa gcggaaatcg cattgcaaaa
    41761 agcgaacgaa aaatttgagc gtcgttttcg cgaagtggag cgtattgttg ccgcgcgtgg
    41821 actggaaatg acaggtgttg acctcgaaac aatggaagaa gtctggcaac aggtaaaacg
    41881 gcaggaaatt gatctctaag ggaattacgc ggtcaagcgc catttgtgtc attttttaaa
    41941 tgacaagcgc ttgatttgcg tcaaaaacat ttaccccaaa ggggctattt tctcactcct
    42001 gatttcaata gtgcgctggc gaagaggagg gataatgaaa gtttgtggca caggtcatgt
    42061 tcgggtatac tgctttcccg tcctggttat tccatcgtct tttcaaccta acttctcagg
    42121 ttcagcatga caacgaacta tatttttgtg accggcgggg tcgtatcctc tctgggtaaa
    42181 ggcattgccg cagcctccct cgcagccatt cttgaagccc gtggcctcaa tgtgaccatc
    42241 atgaaactgg atccgtacat caacgtcgat ccaggtacta tgagcccaat ccaacacggg
    42301 gaagtgttcg ttactgaaga cggcgctgaa accgacctgg acctggggca ctacgagcgt
    42361 ttcattcgta ccaaaatgag ccgccgcaac aacttcacca cgggtcgtat ctactctgac
    42421 gttctgcgta aagaacgccg cggtgactac ctcggcgcaa ccgtgcaggt tattccgcac
    42481 atcactaacg caatcaaaga gcgcgtgctg gaaggtggcg aaggtcatga cgtagtactg
    42541 gtagaaatcg gcggtacagt aggtgatatc gaatccttgc cgttcctcga agcgattcgc
    42601 cagatggctg ttgaaattgg ccgtgagcac actctgttta tgcacctgac gctggtgccg
    42661 tacatggcag cgtctggtga agtcaaaacc aaaccgactc agcactctgt aaaagagctg
    42721 ctctccatcg gtatccagcc tgacatcctg atttgtcgtt cagatcgcgc tgttccggcg
    42781 aacgaacgtg cgaagattgc attgttctgt aatgttccgg aaaaagcggt tatttctctg
    42841 aaagacgtcg attccatcta taaaattccg ggcctgttga aatctcaggg gctggacgat
    42901 tatatttgta aacgattcag cttaaactgc ccggaagcga atctgtccga atgggaacag
    42961 gttatcttcg aagaagcgaa cccggtaagt gaagtcacca tcggtatggt cggcaagtac
    43021 attgaactgc cggatgctta taaatcagtg atcgaagcac tgaaacacgg tgggctgaag
    43081 aatcgtgtca gcgtcaacat caaactgatc gattcacaag atgttgaaac gcgcggcgtt
    43141 gaaatcctta aaggtctgga cgcaatcctc gtacctggcg gtttcggcta tcgtggcgta
    43201 gaaggcatga ttacgaccgc gcgttttgcg cgtgagaaca atattcctta tctgggcatt
    43261 tgcctgggta tgcaggtggc gttaattgat tacgctcgcc atgttgccaa catggagaac
    43321 gccaactcta cggaatttgt gccagactgt aagtacccgg ttgtggcgct gattaccgag
    43381 tggcgcgatg aaaacggcaa cgttgaagtt cgtagcgaga agagcgatct cggcggtacc
    43441 atgcgtctcg gcgcacagca gtgccagttg gttgacgata gcctggttcg ccagctgtac
    43501 aatgcgccga caattgttga gcgtcatcgt caccgttacg aagtcaacaa catgctgttg
    43561 aaacagattg aagatgcagg tctgcgcgtt gcgggccgtt ccggggatga tcagttggtc
    43621 gagatcatcg aagttccgaa tcacccgtgg ttcgtggctt gccagttcca tccggagttt
    43681 acttctactc cacgtgatgg tcacccgctg tttgcaggct ttgtgaaagc cgccagcgag
    43741 ttccagaaac gtcaggcgaa gtaagtaaaa aagttagaac ggcaacgcgt accctgggta
    43801 cgcgttgttt gtctggagtt tcagtttaac tagtgacttg aggaaaacct aatgtccaaa
    43861 atcgtaaaaa tcatcggtcg tgaaatcatc gactcccgtg gtaacccgac tgttgaagcc
    43921 gaagtacatc tggagggtgg tttcgtcggt atggcagctg ctccgtcagg tgcttctact
    43981 ggttcccgtg aagctctgga actgcgcgat ggcgacaaat cccgtttcct gggtaaaggc
    44041 gtaaccaaag ctgttgctgc ggtaaacggc ccgatcgctc aggcgctgat tggcaaagat
    44101 gctaaagatc aggctggcat tgacaagatc atgatcgacc tggacggcac cgaaaacaaa
    44161 tccaaattcg gcgcgaacgc aatcctggct gtatctctgg ctaacgccaa agctgctgca
    44221 gctgctaaag gtatgccgct gtacgagcac atcgctgaac tgaacggtac tccgggcaaa
    44281 tactctatgc cggttccgat gatgaacatc atcaacggtg gtgagcacgc tgacaacaac
    44341 gttgatatcc aggaattcat gattcagccg gttggcgcga aaactgtgaa agaagccatc
    44401 cgcatgggtt ctgaagtttt ccatcacctg gcaaaagttc tgaaagcgaa aggcatgaac
    44461 actgcagttg gtgacgaagg tggctatgcg ccgaacctgg gttccaacgc tgaagctctg
    44521 gctgttatcg ctgaagctgt taaagctgct ggttatgaac tgggcaaaga catcactctg
    44581 gcgatggact gcgcagcttc tgaattctac aaagacggta aatacgttct ggctggcgaa
    44641 ggcaacaaag cgttcacctc tgaagaattc actcacttcc tggaagaact gaccaaacag
    44701 tacccgatcg tttctatcga agacggtctg gacgaatctg actgggacgg tttcgcatac
    44761 cagaccaaag ttctgggcga caaaatccag ctggttggtg acgacctgtt cgtaaccaac
    44821 accaagatcc tgaaagaagg tatcgaaaaa ggtatcgcta actccatcct gatcaaattc
    44881 aaccagatcg gttctctgac cgaaactctg gctgcaatca agatggcgaa agatgctggc
    44941 tacactgcag ttatctctca ccgttctggc gaaactgaag acgctaccat cgctgacctg
    45001 gctgttggta ctgctgcagg ccagatcaaa actggttcta tgagccgttc tgaccgtgtt
    45061 gctaaataca accagctgat tcgtatcgaa gaagctctgg gcgaaaaagc accgtacaac
    45121 ggtcgtaaag agatcaaagg ccaggcataa gactgacttt atctgattta aaaatgccag
    45181 cctccgggct ggcatttttt atggttgagt taccagcggc cggatgcgcc gccacctcca
    45241 gaagaaccgc caccgccgct aaatccgccg ccacttgagc ctccaccgga accgctgttg
    45301 tctgaatgat aacttttacc tgatgctctt ttctgaagta cacataaaca ggtaaagacc
    45361 ataaatatgg tgaaagtaaa aaagaaggaa aatacagcaa tggaaaaaat tttatcggag
    45421 aacaggaata aatctaaaag atagattgct gaaatataga ctccactttt acatgcgcga
    45481 caaaaattac tgccttgatg aaaaaaccag aaagggaaga acatcactgc aaatacccaa
    45541 aaaatagcaa agtaataatg atcactggaa gacgctgatt cactttctga aggattagtc
    45601 ggatattggt gttgtgaagt gagttggtta ttcaaagcgt ttatagctag ctctaatccc
    45661 tgagcaaatt tttgttgttt aaatgcgggt atcatgttgc tacggatgat atctccagcc
    45721 agagcatcgg ttaccttttc ttccagccca taacctacct ggatgcggac agtacgatct
    45781 gaccaggcaa caatgatcag tatcccatca ttacgtttgg catctcctaa acgccaattg
    45841 tcaaaaactc ttgtagcata ttgttcaatt gtctcgtctt tggttgtggg aacaattaat
    45901 acagcaattt gcacttgggt attattgaaa atagattcta acttgtgcgt cagttctttc
    45961 ttttcttgtg agtttaagct actcgtaaga tccgtaactt gttgctgtaa ttgtgggaca
    46021 atagttggtt gggctgcaac aaaggaacac acgaaagtga acattagtat gaaatagcgc
    46081 attataaatt tct
//
LOCUS       DAZFWL020000021        45397 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFWL020000021 DAZFWL020000000
VERSION     DAZFWL020000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 45397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.021"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            352..936
                     /gene="yadN"
                     /locus_tag="ACHKNP_RS06640"
                     /old_locus_tag="pgaptmp_001328"
     CDS             352..936
                     /gene="yadN"
                     /locus_tag="ACHKNP_RS06640"
                     /old_locus_tag="pgaptmp_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4703526.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            1041..1781
                     /gene="yadV"
                     /locus_tag="ACHKNP_RS06645"
                     /old_locus_tag="pgaptmp_001329"
     CDS             1041..1781
                     /gene="yadV"
                     /locus_tag="ACHKNP_RS06645"
                     /old_locus_tag="pgaptmp_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU4703527.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1816..4416
                     /gene="htrE"
                     /locus_tag="ACHKNP_RS06650"
                     /old_locus_tag="pgaptmp_001330"
     CDS             1816..4416
                     /gene="htrE"
                     /locus_tag="ACHKNP_RS06650"
                     /old_locus_tag="pgaptmp_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HGU4703528.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4433..5002
                     /gene="yadM"
                     /locus_tag="ACHKNP_RS06655"
                     /old_locus_tag="pgaptmp_001331"
     CDS             4433..5002
                     /gene="yadM"
                     /locus_tag="ACHKNP_RS06655"
                     /old_locus_tag="pgaptmp_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4703529.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLSGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            5017..5619
                     /gene="yadL"
                     /locus_tag="ACHKNP_RS06660"
                     /old_locus_tag="pgaptmp_001332"
     CDS             5017..5619
                     /gene="yadL"
                     /locus_tag="ACHKNP_RS06660"
                     /old_locus_tag="pgaptmp_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HGU4703530.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            5646..6242
                     /locus_tag="ACHKNP_RS06665"
                     /old_locus_tag="pgaptmp_001333"
     CDS             5646..6242
                     /locus_tag="ACHKNP_RS06665"
                     /old_locus_tag="pgaptmp_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGU4703531.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            6226..7569
                     /locus_tag="ACHKNP_RS06670"
                     /old_locus_tag="pgaptmp_001334"
                     /pseudo
     CDS             6226..7569
                     /locus_tag="ACHKNP_RS06670"
                     /old_locus_tag="pgaptmp_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            7682..8476
                     /gene="panB"
                     /locus_tag="ACHKNP_RS06675"
                     /old_locus_tag="pgaptmp_001335"
     CDS             7682..8476
                     /gene="panB"
                     /locus_tag="ACHKNP_RS06675"
                     /old_locus_tag="pgaptmp_001335"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HGU4703532.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8488..9339
                     /gene="panC"
                     /locus_tag="ACHKNP_RS06680"
                     /old_locus_tag="pgaptmp_001336"
     CDS             8488..9339
                     /gene="panC"
                     /locus_tag="ACHKNP_RS06680"
                     /old_locus_tag="pgaptmp_001336"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HGU4703533.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIIRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9413..10315)
                     /gene="rpnC"
                     /locus_tag="ACHKNP_RS06685"
                     /old_locus_tag="pgaptmp_001337"
     CDS             complement(9413..10315)
                     /gene="rpnC"
                     /locus_tag="ACHKNP_RS06685"
                     /old_locus_tag="pgaptmp_001337"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HGU4703534.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            10589..10969
                     /gene="panD"
                     /locus_tag="ACHKNP_RS06690"
                     /old_locus_tag="pgaptmp_001338"
     CDS             10589..10969
                     /gene="panD"
                     /locus_tag="ACHKNP_RS06690"
                     /old_locus_tag="pgaptmp_001338"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HGU4703535.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10973..12202)
                     /gene="yadE"
                     /locus_tag="ACHKNP_RS06695"
                     /old_locus_tag="pgaptmp_001339"
     CDS             complement(10973..12202)
                     /gene="yadE"
                     /locus_tag="ACHKNP_RS06695"
                     /old_locus_tag="pgaptmp_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU4703536.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12266..12706)
                     /gene="yadI"
                     /locus_tag="ACHKNP_RS06700"
                     /old_locus_tag="pgaptmp_001340"
     CDS             complement(12266..12706)
                     /gene="yadI"
                     /locus_tag="ACHKNP_RS06700"
                     /old_locus_tag="pgaptmp_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU4703537.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDLAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12811..13581)
                     /gene="yadH"
                     /locus_tag="ACHKNP_RS06705"
                     /old_locus_tag="pgaptmp_001341"
     CDS             complement(12811..13581)
                     /gene="yadH"
                     /locus_tag="ACHKNP_RS06705"
                     /old_locus_tag="pgaptmp_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4703538.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13578..14504)
                     /gene="yadG"
                     /locus_tag="ACHKNP_RS06710"
                     /old_locus_tag="pgaptmp_001342"
     CDS             complement(13578..14504)
                     /gene="yadG"
                     /locus_tag="ACHKNP_RS06710"
                     /old_locus_tag="pgaptmp_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4703539.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14613..15275
                     /gene="can"
                     /locus_tag="ACHKNP_RS06715"
                     /old_locus_tag="pgaptmp_001343"
     CDS             14613..15275
                     /gene="can"
                     /locus_tag="ACHKNP_RS06715"
                     /old_locus_tag="pgaptmp_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HGU4703540.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15316..15852)
                     /gene="hpt"
                     /locus_tag="ACHKNP_RS06720"
                     /old_locus_tag="pgaptmp_001344"
     CDS             complement(15316..15852)
                     /gene="hpt"
                     /locus_tag="ACHKNP_RS06720"
                     /old_locus_tag="pgaptmp_001344"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGU4703541.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            16058..18448
                     /gene="gcd"
                     /locus_tag="ACHKNP_RS06725"
                     /old_locus_tag="pgaptmp_001345"
     CDS             16058..18448
                     /gene="gcd"
                     /locus_tag="ACHKNP_RS06725"
                     /old_locus_tag="pgaptmp_001345"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HGU4703542.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18495..20045)
                     /gene="cueO"
                     /locus_tag="ACHKNP_RS06730"
                     /old_locus_tag="pgaptmp_001346"
     CDS             complement(18495..20045)
                     /gene="cueO"
                     /locus_tag="ACHKNP_RS06730"
                     /old_locus_tag="pgaptmp_001346"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HGU4703543.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20211..20558
                     /gene="yacC"
                     /locus_tag="ACHKNP_RS06735"
                     /old_locus_tag="pgaptmp_001347"
     CDS             20211..20558
                     /gene="yacC"
                     /locus_tag="ACHKNP_RS06735"
                     /old_locus_tag="pgaptmp_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HGU4703544.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20664..21530
                     /gene="speE"
                     /locus_tag="ACHKNP_RS06740"
                     /old_locus_tag="pgaptmp_001348"
     CDS             20664..21530
                     /gene="speE"
                     /locus_tag="ACHKNP_RS06740"
                     /old_locus_tag="pgaptmp_001348"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGU4703545.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21546..22340
                     /gene="speD"
                     /locus_tag="ACHKNP_RS06745"
                     /old_locus_tag="pgaptmp_001349"
     CDS             21546..22340
                     /gene="speD"
                     /locus_tag="ACHKNP_RS06745"
                     /old_locus_tag="pgaptmp_001349"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HGU4703546.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22378..22740)
                     /gene="yacL"
                     /locus_tag="ACHKNP_RS06750"
                     /old_locus_tag="pgaptmp_001350"
     CDS             complement(22378..22740)
                     /gene="yacL"
                     /locus_tag="ACHKNP_RS06750"
                     /old_locus_tag="pgaptmp_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HGU4703547.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22915..25512)
                     /gene="acnB"
                     /locus_tag="ACHKNP_RS06755"
                     /old_locus_tag="pgaptmp_001351"
     CDS             complement(22915..25512)
                     /gene="acnB"
                     /locus_tag="ACHKNP_RS06755"
                     /old_locus_tag="pgaptmp_001351"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HGU4703548.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHSL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25867..27624
                     /gene="yacH"
                     /locus_tag="ACHKNP_RS06760"
                     /old_locus_tag="pgaptmp_001352"
     CDS             25867..27624
                     /gene="yacH"
                     /locus_tag="ACHKNP_RS06760"
                     /old_locus_tag="pgaptmp_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HGU4703549.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(27695..29119)
                     /gene="lpdA"
                     /locus_tag="ACHKNP_RS06765"
                     /old_locus_tag="pgaptmp_001353"
     CDS             complement(27695..29119)
                     /gene="lpdA"
                     /locus_tag="ACHKNP_RS06765"
                     /old_locus_tag="pgaptmp_001353"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGU4703550.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29444..31336)
                     /gene="aceF"
                     /locus_tag="ACHKNP_RS06770"
                     /old_locus_tag="pgaptmp_001354"
     CDS             complement(29444..31336)
                     /gene="aceF"
                     /locus_tag="ACHKNP_RS06770"
                     /old_locus_tag="pgaptmp_001354"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HGU4703551.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31351..34014)
                     /gene="aceE"
                     /locus_tag="ACHKNP_RS06775"
                     /old_locus_tag="pgaptmp_001355"
     CDS             complement(31351..34014)
                     /gene="aceE"
                     /locus_tag="ACHKNP_RS06775"
                     /old_locus_tag="pgaptmp_001355"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HGU4703552.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34175..34939)
                     /gene="pdhR"
                     /locus_tag="ACHKNP_RS06780"
                     /old_locus_tag="pgaptmp_001356"
     CDS             complement(34175..34939)
                     /gene="pdhR"
                     /locus_tag="ACHKNP_RS06780"
                     /old_locus_tag="pgaptmp_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HGU4703553.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            35480..36853
                     /gene="aroP"
                     /locus_tag="ACHKNP_RS06785"
                     /old_locus_tag="pgaptmp_001357"
     CDS             35480..36853
                     /gene="aroP"
                     /locus_tag="ACHKNP_RS06785"
                     /old_locus_tag="pgaptmp_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HGU4703554.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAMIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(36896..37750)
                     /gene="ampE"
                     /locus_tag="ACHKNP_RS06790"
                     /old_locus_tag="pgaptmp_001358"
     CDS             complement(36896..37750)
                     /gene="ampE"
                     /locus_tag="ACHKNP_RS06790"
                     /old_locus_tag="pgaptmp_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HGU4703555.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(37747..38298)
                     /gene="ampD"
                     /locus_tag="ACHKNP_RS06795"
                     /old_locus_tag="pgaptmp_001359"
     CDS             complement(37747..38298)
                     /gene="ampD"
                     /locus_tag="ACHKNP_RS06795"
                     /old_locus_tag="pgaptmp_001359"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HGU4703556.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            38386..39279
                     /gene="nadC"
                     /locus_tag="ACHKNP_RS06800"
                     /old_locus_tag="pgaptmp_001360"
     CDS             38386..39279
                     /gene="nadC"
                     /locus_tag="ACHKNP_RS06800"
                     /old_locus_tag="pgaptmp_001360"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGU4703557.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            39482..39922
                     /gene="ppdD"
                     /locus_tag="ACHKNP_RS06805"
                     /old_locus_tag="pgaptmp_001361"
     CDS             39482..39922
                     /gene="ppdD"
                     /locus_tag="ACHKNP_RS06805"
                     /old_locus_tag="pgaptmp_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HGU4703558.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            39932..41317
                     /gene="gspE"
                     /locus_tag="ACHKNP_RS06810"
                     /old_locus_tag="pgaptmp_001362"
     CDS             39932..41317
                     /gene="gspE"
                     /locus_tag="ACHKNP_RS06810"
                     /old_locus_tag="pgaptmp_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HGU4703559.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            41307..42509
                     /gene="hofC"
                     /locus_tag="ACHKNP_RS06815"
                     /old_locus_tag="pgaptmp_001363"
     CDS             41307..42509
                     /gene="hofC"
                     /locus_tag="ACHKNP_RS06815"
                     /old_locus_tag="pgaptmp_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HGU4703560.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(42544..43587)
                     /gene="guaC"
                     /locus_tag="ACHKNP_RS06820"
                     /old_locus_tag="pgaptmp_001364"
     CDS             complement(42544..43587)
                     /gene="guaC"
                     /locus_tag="ACHKNP_RS06820"
                     /old_locus_tag="pgaptmp_001364"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGU4703561.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTTVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(43743..43787)
                     /locus_tag="ACHKNP_RS06825"
                     /old_locus_tag="pgaptmp_001365"
     CDS             complement(43743..43787)
                     /locus_tag="ACHKNP_RS06825"
                     /old_locus_tag="pgaptmp_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703562.1"
                     /translation="MIIKTLKMSARKRN"
     gene            43812..44432
                     /gene="coaE"
                     /locus_tag="ACHKNP_RS06830"
                     /old_locus_tag="pgaptmp_001366"
     CDS             43812..44432
                     /gene="coaE"
                     /locus_tag="ACHKNP_RS06830"
                     /old_locus_tag="pgaptmp_001366"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGU4703563.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            44432..45175
                     /gene="zapD"
                     /locus_tag="ACHKNP_RS06835"
                     /old_locus_tag="pgaptmp_001367"
     CDS             44432..45175
                     /gene="zapD"
                     /locus_tag="ACHKNP_RS06835"
                     /old_locus_tag="pgaptmp_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HGU4703564.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            45185..45382
                     /gene="yacG"
                     /locus_tag="ACHKNP_RS06840"
                     /old_locus_tag="pgaptmp_001368"
     CDS             45185..45382
                     /gene="yacG"
                     /locus_tag="ACHKNP_RS06840"
                     /old_locus_tag="pgaptmp_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HGU4703565.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
BASE COUNT        11383 a        11765 c        11025 g        11224 t
ORIGIN      
        1 ataaatattt tctgaaaatc atacatcctc tctttcctta ataaaaattt tagtttaaac
       61 atttttgcag aaacataata taaattcccg cagataaata tcaatcccat cgccataccc
      121 taaccaattg cacttgtgaa tttatttttg cttaattttg aattaagaaa atttaatttg
      181 ttcaaaagcc cttaaagtgc acaataggcg tcctaatgaa caatggttgt aaattttggt
      241 aacaaatatc attgctcatt aatatctagc atctgcttat aacatttgtg tcatgaatag
      301 tcattcttca aaccagtagt ctgactaatg gaattttatt caaggatttt tatgtctaaa
      361 aaattaggtt ttgccctgtc tggtcttatg ctggcaatgg ttgcaggtac tgcttctgct
      421 gatatggatg gtggtcagtt aaatatcagt ggtctggttg ttgataacac ctgtgaaacc
      481 cgcgttgacg gcggtaataa agatggtctg atcctgctgc aaactgcaac tgttggtgaa
      541 atcgatgctg gcgtgctgaa tgacaccgtt ggcgctaaag ccaaaccttt cagcatcacc
      601 gttgactgca gcaaagcaaa tccgaatcca ggtagtacag ctaaaatgac cttcggttct
      661 gttttctttg gtaatagcaa aggaacgctg aacaacgaca tgtctattaa caacccgtct
      721 gacggcgtga atatcgctct gcacaatatt gatggttcca ctatcaaaca ggttcaaatt
      781 aacaaccctg gcgatgtgta tactaaagcc ctggatgcaa cgacaaaatc tgctgtttat
      841 gattttaaag cgtcttacgt tcgtgccgtc gcagaccaaa cagcaactgc tggttatgta
      901 aaaactaaca ctgcatacac cattacttat cagtaagtat tcaccaatta aattgattga
      961 atgaatatac agggaatagt gttaataatt tctattttta tattattccc tgttttaatt
     1021 aactctgtca gggatggttt atgtttttta acaccaaaca tacaacagct ttatgcttcg
     1081 taacctgtat ggcttttagt tcatcgtcta ttgcggacat tgtcatttcg ggtactcgcg
     1141 taatatataa aagcgatcaa aaaagtgtca acgtacgtct ggaaaataaa gggaataacc
     1201 cgttgcttgt ccagagttgg ttagatactg gcgatgacaa cgctgagcct ggcagtatta
     1261 cagtcccttt tactgccacg ccgccagtat cgcgtattga tgccaaacgt gggcaaacaa
     1321 tcaaattaat gtacacagcc agcacctcac tgcctaaaga cagagagagc gtgttctggt
     1381 ttaacgtact ggaagttcca ccaaaaccag atgcagaaaa ggtcgcgaat caaagtctgc
     1441 tgcaactggc atttcgcaca cgtataaaac ttttctatcg cccggatgga ttgaagggaa
     1501 atccctctga agccccgtta gccctgaagt ggttctggtc aggttcagaa ggtaaggcgt
     1561 cattgcgagt gaccaatcca accccttact acgtctcttt tagcagtggt gatttagaag
     1621 ctagcggtaa acgctatccg attgatgtga aaatgattgc accatttagt gatgaggtca
     1681 tgaaagtcac tggccttaat ggcaaagcaa gttctgcaaa agtacatttt tacgccatta
     1741 atgattttgg tggcgccatt gaaggtaatg ccagtctgta atcaggcaag gatataattc
     1801 cgcaggaagc ataacgtgac tataaaacca actaaaaatt atcataatca tctgacccgt
     1861 atcgctacgt tttgcgcgct gctgtattgc aatagtgctt tctgtgctga acttgttgaa
     1921 tatgaccata ccttcctgat ggggcagaat gcctcaaata ttgatctcag ccggtatagt
     1981 gaaggtaacc ctgctatacc gggtatgtac gacgtcagtg tttatgtcaa cgatcaacct
     2041 attattaacc aaagcattac atttattgaa attgaaggaa aaaaaaacgc acaagcctgt
     2101 atcacactta agaatttatt gcagtttcat ataaattctc ccgatataaa taacgaaaaa
     2161 gccgttctgc ttgccaggga tgaaacactc ggcaattgcc ttaatttgac ggaaattatc
     2221 cctcaggctt ctgttcgtta tgacgttaac gagcaacgtc tggatataga cgttcctcag
     2281 gcatgggtaa tgaaaaatta ccaaaactat gttgatccat cgttatggga aaatggcatt
     2341 aatgcggcca tgttgtcata caacctcaac ggatatcata gtgaaactcc tggtcgaaga
     2401 aatgacagca tttatgctgc ttttaacggt gggatgaatt taggtgcatg gcgactgcgt
     2461 gcctcaggca actacaactg gatgaccgat tctggcagta attatgattt taagaatcgg
     2521 tatatacagc gtgatatcgc ctcgctgcgt tctcaactca ttcttggtga gtcttataca
     2581 acgggcgaaa cctttgattc tgtcagtatc cgcggcattc gtttatacag cgacagccgc
     2641 atgttgcctc cgacattagc gagttttgcg cctatcattc atggcgttgc taataccaac
     2701 gccaaagtca ctattacgca aggtggctat aagatttatg aaacgactgt gccgccaggc
     2761 gctttcgtca ttgatgatct gagtccgtca ggttacggca gcgatcttat tgttactgtc
     2821 gaagaatccg atggctcaaa gcggacattc tcgcaacctt tttcatcggt cgttcaaatg
     2881 ttacgccctg gcgttggacg ttgggatatt agcggcggcc aggtcttaaa agatgatatt
     2941 caggatgagc ctaatttatt tcaggccagc tactactacg gtctgaataa ctatctaacg
     3001 ggttatactg gtattcagat taccgataat aactataccg ctggtttgtt aggtcttggt
     3061 ctgaatactt cagtcggtgc attttctttc gatgtgactc actccaatgt tcgtataccg
     3121 gatgataaaa catatcaggg acaaagttat cgtgtttcct ggaacaagtt attcgaagaa
     3181 acaagtactt cactgaatat cgcggcctat cgctattcga cacagaatta ccttggtctt
     3241 aatgatgcac taactctaat tgatgaagtg aaacatcccg aacaagatct tgaaccgaaa
     3301 tccatgcgta attactcgcg catgaaaaat caggttacgg tcagtattaa ccaaccgcta
     3361 aaatttgaga aaaaagatta cggttcattt tatctttccg gaagttggtc cgattactgg
     3421 gcttccggac aaaatcgtag caattactct attggctaca gtaacagtgc atcctggggc
     3481 agctacagtg tcagtgccca gcgttcatgg aatgaagacg gcgacactga cgatagcgtt
     3541 tatctcagtt tcaccattcc tattgaaaaa ttacttggca ctgaacaacg gacttcaggt
     3601 ttccagagta ttgatactca aatgagcagt gactttaagg gtaataacca actcaacgtc
     3661 agtagcagtg gctatagtga taacgctcgc gtcagttata gcgtaaatac tggctatacg
     3721 atgaataaag ccagcaaaga tttgagttat gttgggggtt atgccagcta tgaatcaccg
     3781 tggggaaccc tggcaggttc agtttctgca aatagcgata acagtcgtca agtgtctctc
     3841 agcaccgacg gtggttttgt attgcatagc ggcggcctga ccttcagtaa tgacagtttt
     3901 agcgactccg atacactggc ggtagttcag gctccaggtg ctcaaggagc gcgaataaat
     3961 tatggcaaca gtactatcga tcgatggggt tatggtgtca ccagcgctct ttctccttat
     4021 catgaaaacc gtatcgcgct ggatatcaac gatcttgaga acgatgttga attaaaaagt
     4081 accagtgcag tagctgtacc gcgtcagggt tcagtcgtct ttgctgattt tgaaaccgtg
     4141 caagggcaat cagccattat gaacatcaca cgaagtgatg gtaaaaatat tccatttgct
     4201 gcagatattt atgatgagca aggcaatgtc attggtaatg ttggacaggg tggacaagca
     4261 tttgttcgtg gtattgagca gcagggaaat atcagcatta aatggctcga agaaagtaaa
     4321 cccgtaagtt gtcttgcgca ttatcaacaa agttcagaag cagaaaaaat agcgcaatct
     4381 attattctga atggaattcg gtgtcagatt cagtaactac aagggacgtc aaatgataaa
     4441 aataacgcca cataaaatag cgatactgat gggaatatta ttatcaccat cagtatttgc
     4501 aacggatatt aatgtagagt ttacagccac tgtcaaagcg acaacctgta acatcacact
     4561 tactggtaat aacgtcacga atgatggcaa taataactac acattgagaa tccctaagat
     4621 gggtctggat aagatcgcga ataaaacgac agaatctcag gctgatttta aactggttgc
     4681 tagtgggtgc agcagtggca tcagttggat tgataccact ctgagcggaa atgcatcatc
     4741 aagctcacct aagcttatta taccgcagtc tggtgattca tcttcgacga caagtaatat
     4801 cggtatgggt ttcaaaaaac ggactactga tgatgccact ttccttaaac ctaacagtgc
     4861 ggaaaagata cgctggagca cagacgagat gcagcccgat aagggtcttg aaatgaccgt
     4921 tgcgctacgt gaaacagatg cagggcaagg cgtaccgggg aattttcgtg cactggcaac
     4981 gtttaatttc atctatcaat aagaaggata attaagatga catttaaaaa tttacgttat
     5041 ggattatcca gcagtgtcgt tttggctgcc tcattgttca gcgtaccctc ttatgcggca
     5101 acagacagca ttgggctgac cgtgattact accgtagaaa taggtacttg taccgctaca
     5161 ttagtaaatg actctgatca ggacatttct gttgttgact ttggcgatgt atatatttct
     5221 gaaatcaatg ccaagaccaa agttaaaaca ttcaaactca aattcaaaga ctgtgcgggt
     5281 atccccaata aaaaagcaca aataaaatta accaagcgag ccacatgcga aggtactgct
     5341 aatgacggtg cggggttcgc aaatggatcc acagccgcag ataaagcaag tgccgtcgcc
     5401 gttgaagtct ggagcaccga aactccggca acagggagcg caacacaatt tagttgtgtg
     5461 acaccagcat ctcaagaggt cactatctcc aatgcagcca acgcggttgt ttattatccg
     5521 atgagtgctc gactggtcgt cgaaaaaaat aaaaccgtaa gcaatgtcac tgcgggtaaa
     5581 ttttctgcac ctgccacatt tacagtaacc tataactaac agactgacag atccaggaac
     5641 tatatatgca tcccactcaa cgtaagctga tgaagagaat aattctgttt ctgtcactac
     5701 agtttcccat tacctatcca gccatcgctg ggcaggatat tgaccttgtt gccaatgtaa
     5761 aaaacagcac ttgcaaaagc ggaatcagta accagggtaa tattgatctt ggcgtcgttg
     5821 gggtgggata tttttcagat aatgttactc ctgaaagcta tcaaccaggt ggaaaagagt
     5881 tcactgtcac agtatcagac tgtgcattac agggaactgg cgatgtgctt aatcagttac
     5941 atattgattt tagagccctt agcggtgtca tggctgcagg ttcaaggcaa atatttgcca
     6001 atgaagtcgc aaccggggct aaaaatgtcg gtgtcgttct cttttccatt caggacccga
     6061 ccaatatatt caatgttatt agttcagcag gaaattctcg ttccgtgtat ccagtaatga
     6121 cttctgccct gcataattct tcatggaaat tttatgccag aatgcaaaaa atcgatcccg
     6181 cgctggatgt catatctggc caggtaatga gtcatatatt agttgatgtg tactacgaat
     6241 aatagatcaa aacaaataac tgcgactgtt acagcagtag atggtggagt gataatgaag
     6301 aaattcttca gacattttct ttttttaatt ctgtgcttga gttgttatac cgcgagtgct
     6361 ggcactgatg ataacgtagg atatattgtt gggaatagtt acggcgttgg tccgtcagat
     6421 caaaaatgga gagaaacggg gccgaatggc gatgctactg tcatattcag atatgcaaca
     6481 agtacaaata atttagtttt ctacaaaccg acgcagcttg gacctacagg tgtaaaatta
     6541 cagtggagtc agttagatac cgcttctggt ggtggttttc tttattgcaa cagaagtgat
     6601 agcacaagtg gtagcgcaat gcgtattgaa aatgcaatgg ttgactcagg taaaatgtat
     6661 ggctcccata aattatttaa tacatcagtt cctggtttgt attacacatt attaatttca
     6721 aacatgtggt cagcttacgg taccgtaact aacgttagtt cacctgggat atacattggt
     6781 gactctgcag aacaatattt ttcgtggtat aatccaagcg aagacgtgtt atattagagt
     6841 tgcaataatg cgaatagcac ccgtaaatac tgggctgtag gtggtattta tcagaccctt
     6901 acaattgaat tctatacaga tacaaacttt gatccaacag taactcaaca gatcaaatta
     6961 tccagctcat caaattatct gtattcattt aaagcctatg gcgcaggcca aggtataaat
     7021 gagcatagtt attttatcaa aatcgatttc gatctgctca atgtcaagtt gactaatccc
     7081 acttgtttta ccgctatgct tagtggaact tctgtgactg gttctacggt aaaaatgggt
     7141 gaatatagtg cagaacaaat cagaaacggt gccacaccgg ttccttttga tatttcactt
     7201 caaaactgcg ttcgtgtgac taatattgag acaaaattag tttcaacaaa ggttggtact
     7261 gaaaacgggc aactccttgg taatactctc acaggtaatg acgcagcaaa aggagtcggt
     7321 gtactcatag aaggtttagc aactagtaaa aatcctctaa tgacattgaa acctaatgat
     7381 tcaaattctg tttataaaga ttacgacccg agaggcaaag acgatacaac aggaggagtt
     7441 tacccggatc aagatacagg tataacatac cctctccatt tccaggccac gctacaacag
     7501 gatggaacta taccaataga agctggtgag tttaaagcca ccagtacttt ccaggtaacc
     7561 tacccttaat aagtccaccg caccgccatc atctggcggt gcagtaattg ataaatcatt
     7621 gcccccacca atgtgactga taaaatgcca ggcaacacga catcatttat caggatacgt
     7681 tatgaaacca accaccattg cttcacttca gaaatgtaag caggataaaa agcgtttcgc
     7741 gaccatcacc gcttacgact acagcttcgc gaaactattt gctgatgaag gacttaacgt
     7801 catgctggta ggcgactcgc tgggcatgac ggttcagggt cacgactcca ccctgccggt
     7861 gaccgttgct gatatcgcct accacactgc cgccgtacgt cgcggcgcgc caaactgcct
     7921 gctgctggct gatctgccgt ttatggcgta tgccacgccg gaacaagcct ttgaaaacgc
     7981 cgcaacggtt atgcgtgccg gtgccaatat ggtcaaaatt gagggcggtg agtggctggt
     8041 cgaaaccgta aaaatgctga ccgaacgtgc cgttcctgta tgtggtcact taggtttaac
     8101 cccacagtca gtgaatattt tcggtggcta caaagttcag gggcgcggcg atgaagcggg
     8161 cgatcaactg ctcagcgatg cattagcctt agaagccgct ggggcacagc tgctggtgct
     8221 ggaatgcgtg ccggttgaac tggcaaaacg tattaccgat gcactggcga tcccggttat
     8281 tggcattggc gcaggcaacg tcactgacgg gcagatcctc gtgatgcacg acgccttcgg
     8341 cattaccggc ggtcacattc ctaaattcgc taaaaatttc ctcgccgaaa cgggcgacat
     8401 ccgcgcggct gtgcggcagt atatggctga agtggagtcc ggcgtttatc cgggcgaaga
     8461 acacagtttc cattaaggag tcacgttgtg ttaattatcg aaaccctgcc gctgctgcgt
     8521 cagcaaattc gccgcctgcg tatggaaggc aagcgcgtgg cgctggtgcc tactatgggt
     8581 aatctgcacg atggccatat gaagctggtc gacgaagcca aagcccgcgc cgatgtggtc
     8641 gtcgtcagta ttttcgttaa cccaatgcag tttgaccgcc cggaagatct ggctcgttat
     8701 ccacggacct tgcaagagga ctgcgaaaag ctaaacaaac gtaaagtgga tttagttttc
     8761 gccccttcgg taaaagagat ctacccgaac ggtactgaaa cccacactta cgttgacgtt
     8821 cctggccttt cgaccatgct ggaaggtgcc agccgtccgg gacattttcg cggcgtttcg
     8881 actattgtca gcaagctgtt caacctggtc cagccggaca tcgcctgctt cggtgaaaag
     8941 gactttcagc aactggcgct aatccgcaaa atggttgccg atatgggctt cgatattgaa
     9001 attgtcggcg tcccgattat acgcgccaaa gatggccttg cgctcagttc ccgtaatggt
     9061 tatctgacgg cggaacaacg caaaattgcg cccggtctgt acaaagtttt aagttcaatt
     9121 gccgacaaat tgcaggctgg ggaacgggat ctcgatgaaa ttattaccat tgcggggcaa
     9181 gaactgaatg aaaaaggctt ccgcgccgat gatattcaga ttcgcgatgc cgacacattg
     9241 ctggaagttt ctgaaaccag caaacgggca gtaattctgg tagccgcctg gcttggcgat
     9301 gctcgcctga tcgacaacaa aatggtcgag ctggcgtaat acttaactgg cgctacggct
     9361 gatggcgcca gttattaatt taccccacgt catctgtcat aacacaggta acttaaataa
     9421 ggttaattac cttatcaatc tcagcaagag gtaaatttgc catctctgca acgtcttccc
     9481 gagacattcc tttacttaga agacgcaggg cgaattcttg cctttcctcc tgtcttccct
     9541 gctgaatccc cttctcaatc cctttctctt caaaccactg cgccagcgtc atcatagact
     9601 cccctcccgt ttccctgtct ctcagcacac cataaaacaa atccgcttgt tcagtatgac
     9661 cgcgttgcag catatagttt tgcatggcaa ctaactgact tccgctagtg tacccttcgt
     9721 caatcagcgt gaccagttgc tccagcaata acattaagtc gcgctggcga atatgttttt
     9781 gcagtagttc gagaatcgca atccgccgat gttgcatgat ttcgtcatcc ggtgtgatgg
     9841 tgatatccac cagcgggaaa ggactgttat agacgcgtcg tgccagctcc ggcgagtaaa
     9901 atatatcaaa ccagcacatt gagagcggat aaggtgtggc ctcgccctga taaaacagta
     9961 tcggcaccac cagcggcagc ttatcgtgat ccgcctccag atgccggtgc atggcagcaa
    10021 tagaataacg catcatgcga aaggccattt tcttatccgg cttgctttgg tgttcaatca
    10081 caacatgcag ataaccggga ttgccctgca tttgcacgga atagagcacg tccgtgctgt
    10141 gtcctttcag gctctcttca atgaaactcc ccgactctaa atgaagcgtg ttgaggtcac
    10201 aaagttctcg taattccact ggcaaatgta tctccagaaa gtcgcgagcc gtctccgcgt
    10261 gcattaaaaa ttgtttaaac actgcatcat gcggtgtggt actcggtgca tccatcttcc
    10321 ctgccttccg tgttgtcttg cgtgaaggca gtatccagtc aaggcgtgcc atcgtcagcc
    10381 acctctttag tgtttaccga gcagcgttca gagaaatttc aattggttac agccgttaca
    10441 tggcacgctt cggcacggta cgcaaactgc tgatgtaaat gccgtcagat gagtagacac
    10501 taaacaaaaa tcgggcaata ctgcgtgaga atttcctcaa agcaggcatc gcctgcttcg
    10561 ttaacgacag ggtagaaagg tagaagttat gattcgcacg atgctgcagg gcaaactcca
    10621 ccgcgtgaaa gtgactcatg cggacctgca ctatgaaggt tcttgcgcca ttgaccagga
    10681 ttttcttgac gcagccggta ttctcgaaaa cgaagccatt gatatctgga atgtcaccaa
    10741 cggcaagcgt ttctccactt atgccatcgc ggcagaacgc ggttcgagaa ttatttctgt
    10801 taacggtgcg gcggcccact gcgccagtgt cggcgatatt gtcatcatcg ccagcttcgt
    10861 taccatgcca gatgaagaag ctcgcacctg gcgacccaac gtcgcctatt ttgaaggcga
    10921 caatgaaatg aaacgtaccg cgaaagcgat tccggtacag gttgcttgat tgttatccct
    10981 gcggctggtt actcaccagc cgcgacatcg tctccagcga atccgttctt aagatataaa
    11041 gtcgttttag taacaacgga ttatcccccg gttttacttt gcctttcatg gttgtcaccg
    11101 ccaggtgaaa tccggcatcg tttgctgcct tcacggcttt gtcattaaat ccgccaaacg
    11161 gatacgaaag ataccagaca tgcggattaa attgcgccag agcgcggcgt gaacgtgcaa
    11221 aatcaaacag aatattgtgc tcactacggc tcagtaatat gggtcggcga tagccatcca
    11281 cccgatgcaa aaaatgggta tgtgactgaa aatcaaaaac atcgcgaatt tcgttaagct
    11341 cagaaacgct cataaattgc agcgattttg ggttccactt ctgcgggtga cgtttgatgc
    11401 gcgaggtaac aacaaacgcc gtcgccttca tgccatattg tttcaataca ggatacgcat
    11461 agcggctcac cgacttgagg ccatcatcaa aggtaatcac caccgctcgc gcagggagat
    11521 tgatcttatt cttcacgtaa ccttccagct gcgccatgct cagtgtcgcg tatcccctgt
    11581 cgcgcagcca ggccatttgg ttattgaaag cgcgtaccga ggtggtcgtc gaagtatggc
    11641 gaaaacgggt gttttcttca tcgcgcagaa tatggtgata ggttagcacc ggcaggccat
    11701 tatcgggttg ggcatccagc gcgctgatat aggcgagtcg atcgccaata cggatctgat
    11761 accaggtttg atttaacctg tctttcagtt tatgcaaaat cgggtagcgc aaattgtccg
    11821 ccagtacccc aaatggcgca ctgcccgcac tcggcgcgtt atagaccggc gtatctttcc
    11881 aggtaattaa gttctgatta ctcagcggct tgttgaggtc acccaaaccg tcttcaactt
    11941 tttgtcgccc ctgaactggc tcgagatgcc ctttatcgat aaacccttta ccaaagccaa
    12001 aattaaatgc gtaataactt gcggcagtgg gctccacggc aatgatttgc ccggcccgaa
    12061 tattccccac ggtcaccatc ttgtcgccaa tttgcgccca gatcgcagca ttttcgatgg
    12121 tttgcatata acgggcaggt aacgcggcac tgacacttgc ggtaaacagc atcagcagga
    12181 gaataacagc ttgtttgtac atgaaaaaat aaccgagggt gaaacaacgc ttttattgta
    12241 gcaaaagcgc tataagaatc gtgggttaat actcatacaa attatgtttg aggacgaaag
    12301 gcggattttt ttgttgttgg tgccaaagac tactcacctc cggcccaccc agttcgacaa
    12361 tacgctcgcg aaactctgaa ctggtcatgg tttcacggca agccatcatc tgttcaatta
    12421 acggtaacgt gacaccagaa atcacttcac aacgggagtg tttatggctt aataatgaag
    12481 ccacgcggta cggtggcgct ccggctaaat cggttaagaa gatgacaccc tcaccagagt
    12541 ccgcttcatg cagagcatcg cacatcatgc ggctgagcat attagagctt aatccgcgcc
    12601 agaaattcac ggcccggcac tgaagaagtg ccccatgttt tttctccagc gcatccagta
    12661 tctcttgcgc ccggtcatcg tgacaggtaa ttacccaacc taacatcgcc ttcctcctta
    12721 gccgaaaatc agtctaccct gaccattgtt gataaagggt gatagccgtc aaaactcctc
    12781 tcccccgaat ccagaggaga ggaaatagcc ttagctacgc aaaccacgtc cacgttggat
    12841 cagcgaccaa cagatcaaat aaaacgccac aataaagacc accagtacgc caaaggtagt
    12901 gaccagcggc acatcattta taccgaggaa gccgtagcgg aaaccactga tcatataaac
    12961 gattgggttc aggtgcgaca gcccttgcca gaacggcggc aacaaagtca gggagtaaaa
    13021 caccccgccc aaatacgtga gtggcgttaa cacaaaggtt ggcaccaggc tgatgtcatc
    13081 gaacgttttg gcaaacacgc cgttcagcaa acccgcaagg gagaatagca ccgccgtgag
    13141 caccagggtt aaggcaacga atacccacga atgcacctga aacggcacaa aaaacagtga
    13201 aattgccgtc accagaatgc caacaaacag accacgcgcc acgccaccgc cgacatatcc
    13261 ggcaataatg acgtgagtcg gaaccggcgc taccagcagc tcttcaatat tacgctggaa
    13321 cttggcacca aaaaatgatg acgcgacgtt agcgtaggca ttggtgatca ccgacatcat
    13381 gatcagcccc ggtacgatga actgcatata gctgaagcca tgcatatcgc caatacgcga
    13441 acctatcagg ttaccgaaga taataaagta aagggtcatg gtgatgactg gcggcaccag
    13501 cgtctgcacc cagatacgca taaagcgatg gatctctttc gcccagatgc tttttagcgc
    13561 cacccagtaa agatgcatca tgcgcgatct ccttgttttt cattaaccag tgaaacaaac
    13621 agctcttcca gacggttagc tttgttacgc atacttaata cctgaatgcc ctgctcactt
    13681 aactgcgtaa atacgctgtt gatcccctgc tcacgcagca cttcaacttc cagcgtcgcg
    13741 gtatcgacca gtcgatactg atagccatcg agcttcggta acggactttt cggtgcgaga
    13801 tcgagaataa aggtttccga tttcagctta gccagcagcg ccttcatcga ggtattttcc
    13861 accagctcac cgtgttgaat aatgccgata ttgcggcaca gcatttctgc ttcttccagg
    13921 tagtgtgtgg tgagaatgat ggtggtgcct ttgtcgttta aatccttcaa aaagccccac
    13981 attgagcggc gaagttcaat atccacgcct gcggtcggtt cgtcgagaat cagtagttta
    14041 ggttcatgca ttaacgcacg ggcaatcatt aaacggcgct tcatcccgcc agataacata
    14101 cgcgcacgtt cgttgcgttt tccccataga tcgagctgct taagatattt ttcgctgcgg
    14161 atgtacgcct ctttgcgctc cacaccgtag taccctgcct gattcaccac aatttgctgc
    14221 acggtttcaa acgggttgaa gttaaattcc tgcggcacca gtcccaactg acgtttagcg
    14281 ttcacgacat ccttctcgag atcgtaacca aatacgctga cccgcccgga ggttttattt
    14341 accagagagc tgataatacc gatagtggtc gatttcccgg ccccgttcgg cccgagaagc
    14401 gcataaaaat cacccgcttc gacctgcaaa tctatcccac gaagcgcctg aacgccgcct
    14461 ggataggttt ttttaagctg ttgaagttcc agtgcaatgg tcataaattt ttacttacct
    14521 tacgttctta cactttatct gtggtttaaa tcgtcccgga gttgccctat attagccaaa
    14581 cgtaattatt tggttacagg tcgttaacct ccatgaaaga catagataca ctcatcagca
    14641 acaatgcact atggtcaaaa atgctggtgg aagaggatcc cgggtttttt gagaaactgg
    14701 cacaagcgca aaaaccgcgc tttctatgga ttggatgttc cgacagtcgc gttcctgcag
    14761 aacgtttaac cggtcttgag ccgggcgaac tctttgttca ccgtaatgtt gctaacctgg
    14821 tcattcacac tgacctgaac tgcctttccg tggttcagta tgcagtggat gtactcgaag
    14881 ttgaacacat tattatctgt ggccactacg gttgcggcgg cgtacaagcc gcagttgaaa
    14941 acccggaact ggggcttatc aacaactggc tgctgcatat ccgcgatatc tggttcaaac
    15001 atagctcatt gctcggcgaa atgccgcaag agcgccgtct ggataccttg tgtgaactga
    15061 acgtcatgga acaggtgtat aacctgggcc actccaccat tatgcaatca gcgtggaaac
    15121 gcgggcagaa agttaccatt cacggctggg cctacggcat tcacgacggc ttgctgcgtg
    15181 atctggatgt taccgccacc aaccgcgaaa cccttgagca acgttaccgt cacgggattt
    15241 ccaacctcaa gctgaaacac gccaaccaca aataaaaatg ccatgccgga tgcaacacat
    15301 ccggcaactt cacacttact cgtccagcag aatcactttg ccgatatacg gcagatgacg
    15361 gtaacgctgt gcgtaatcaa tgccgtaacc caccacaaac tcatccggga tcgagaaacc
    15421 gataaattct accgggacgt tcacttcacg acgggacggt ttatccagca gcgtacaaat
    15481 cgccagcgac ttcggttcgc gcaggcttaa gatctcacgc actttcgaca gtgtattccc
    15541 cgagtcgatg atatcttcaa caatcagcac gtccttgcca cggatatctt catccagatc
    15601 tttgaggatt ttcacatcac gggtggtgga catgccgcta ccgtagctgg aggcggtcat
    15661 aaagtcgact tcatgagata cctgaacttc acggcacagg tccgccataa acataaatga
    15721 gccacgcagc agacccacca gcaccatatc gctgccgctg tctttgtaac gctcagtaat
    15781 ctgacgaccc agttcggcga tacgcgcttt aatctccgct tcggggatca ttacttctac
    15841 agtatgtttc atatctctaa ccatatgatt taaaaacaaa tcacttcgca cgggcaggtt
    15901 ttggcaccgc aacaaagcgc aagacacgca gtataccagt aaaactattc attcgcggaa
    15961 tctgttaata aagctcgaat tgtgatgacg atcacacatg ttaaaaccta taattaattg
    16021 ctactaaaat attaatgaat tgaaatggtg tctctttatg gcaattaaca atacaggctc
    16081 gcgacgatta ctcgtcacgc taacagccct ttttgcagcg ctttgcgggc tgtatctact
    16141 cattggcgga ggctggctgg tcgcgattgg cggctcctgg tactacccta tcgctggcct
    16201 tgtgatgctc ggcgtcgcct ggatgctgtg gcgcagtaaa cgtgccgcgc tttggctata
    16261 cgccgccctg ctgctcggca ccatgatttg gggcgtctgg gaagttggtt tcgacttctg
    16321 ggcgctgact ccgcgcagcg atattctggt cttcttcggc atctggctga tcctgccgtt
    16381 tgtctggcgt cgcctggtca ttcctgccag cggcgcagtt gccgcactgg tggtggcact
    16441 gctgattagc ggtagtatcc tgacctgggc cggatttaac gatccgcagg aaatcaacgg
    16501 caccttaagc gccgatgcca cacctgcgga agctatctca cccgttgccg atcaggactg
    16561 gcctgcctat ggtcgcaatc aggaaggtca acgcttttca cctctgaaac aaattaacgc
    16621 cgataacgtc cataatctga aagaagcctg ggtgttccgt actggcgatg tgaagcagcc
    16681 gaacgatccg ggcgaaatca ccaatgaagt gacgccgatt aaagtgggcg acacccttta
    16741 cctgtgtacc gctcaccagc gcctgtttgc gctcgatgcc gccagcggca aagagaaatg
    16801 gcattacgat cctgagctga aaactaacga gtctttccag cacgtaacct gccgtggcgt
    16861 ctcttatcat gaagccaaag cagaaacggc ttcgccggaa gtgatggcgg attgcccgcg
    16921 tcgtatcatt cttccggtca atgatggtcg actgattgcg attaacgctg aaaacggcaa
    16981 actgtgcgaa accttcgcca ataaaggcgt gctcaatctg caaagcaata tgccagacac
    17041 caaaccgggt ctgtatgaac cgacttcgcc accgattatc accgataaaa ccatcgtgat
    17101 ggcaggttca gttaccgata acttctcaac ccgcgaaacg tctggcgtga tccgtggttt
    17161 tgatgtcaac accggggagc tgctgtgggc ttttgatccc ggcgcgaaag atccgaacgc
    17221 aatcccgtct gacgaacaca cctttacctt taactcgcca aactcgtggg cgccagcggc
    17281 ctatgacgcg aagctggatc tggtctatct gccgatgggc gtgaccacgc cggatatctg
    17341 gggcggtaac cgcacaccgg aacaggaacg ttatgccagc tcgattctgg cgctgaatgc
    17401 cactaccggg aaactggcgt ggagctacca gaccgttcac cacgacctgt gggacatgga
    17461 tcttccggca cagccgacgc tggcggacat caccgttaat ggtcagaaag tgccagttat
    17521 ttacgctccg gcgaaaaccg gcaacatttt tgtgctcgat cgtcgcaatg gcgaactggt
    17581 ggttccggcc ccggaaaaac cggtgccgca aggtgctgcg aaaggcgatt acgttactcc
    17641 gacccagccg ttctctgaac tgagcttccg tccgaagaaa gatttgagcg gtgcggatat
    17701 gtggggagcc accatgtttg accaactggt gtgccgcgtg atgttccacc agatgcgcta
    17761 tgaaggcatt ttcacgccgc catctgaaca gggcacgctg gtcttcccgg gtaacctggg
    17821 aatgtttgaa tggggcggga tttccgttga tccaaatcgt gaagtggcaa ttgccaaccc
    17881 aatggcactg ccgtttgttt cgaaactgat cccacgcggt ccgggtaacc cgatggagca
    17941 gccgaaagat gccaaaggca cgggtacgga atccggcatt cagccacagt acggtgtacc
    18001 gtatggtgtc acgctcaacc cgttcctctc accgtttggt ctgccgtgta aacagccagc
    18061 atggggttat atctcggcgc tggatctgaa aaccaatgaa gtggtgtgga agaaacgtat
    18121 tggtacgccg caagacagta tgccgttccc gatgcctgta ccggtgccgt tcaatatggg
    18181 tatgccgatg ctgggcgggc caatctccac ggcgggtaac gtgctgttta tcgctgctac
    18241 ggcagataac tacctgcgcg cttacaacat gagcaacggt gaaaaactgt ggcagggccg
    18301 tttaccagcg ggtggtcagg cgacgccaat gacctatgaa gtgaatggca agcagtatgt
    18361 ggtgatctcc gcaggcggtc acggttcatt tggtacgaag atgggcgact atattgtggc
    18421 ttatgcgctg ccggatgatg tgaaataacc ataatgcccg gagagatccg ggcatatttc
    18481 cgaatacggt ctttttatac cgtaaaccct aacatcatcc ccgtatcttc atgctccagc
    18541 agatggcagt gcgccatata agcatgttct ttcggtgcgt tgtgattaaa cttcaccagc
    18601 acttcactga cattgccttc taccttaacg gtatctttcc agcccgcgcg atgagccgct
    18661 ggcggtttgc cattttctga caagatacgg aactgggtgc cgtgaatatg gaacggatgc
    18721 agcatcatgt cgcccacgcc agagataacc caacgttcgt actgcccttt cgccgccgca
    18781 aacatcggct tattcatatc aaacgcctga ccgttgattt tattggcatg gtggaaatcg
    18841 aacttcccgc catggttcat atgattcata ttgccgtgcc ccatatggcc catcatctgg
    18901 ctatgatcca tcccggccat cgcctgatcg ccatatttct ccatcagcat ctgcatcccc
    18961 atcatatcga gcatcgggtc catagaaagt tgcagcttgc gtaccgtcag cccttccagc
    19021 gaaggtaacg caggcaggct acttaatgtg tctggcaaag caccggaggc actaatagca
    19081 atcggctgaa tccgcattac cggatgcggc ttgtcaaacg gcgcaatcgc catccccatc
    19141 tggctgaccg gtagcgtcac caggtcaaag ggtttgttgt cgttaacctc caccagcact
    19201 tcaaaacgct cgcccatcag caccggcagc tcgctcacct tcaccggttc aggtagcaga
    19261 ccaccgtcgc tggcaatcac atacagcggg cgattgtcgc tggtggcgaa attgagcgag
    19321 cgggcattac agccattaag caaacgcagg cgcagccaac cacgcggggc agcgtgttgt
    19381 gggtagattg caccgttggt cagcagcgta tcgccaaacc agcccacggc ggcggtcatc
    19441 acatccagtt gataatcaat ctgcccatca gcgttaaatt ttttatcctg aacaatcacc
    19501 ggaacatcat cgatgcccca ctgttttggc agcattaatt tcaggatctc gtcatcttca
    19561 attaccacca gcccagccaa ccccatcgcc acctgtcgcc cggtcttgcc atgttgatgt
    19621 ggatggaacc agcaagtagc ggcaggttga tcaacgttca acgtcaccga gcgcttgcca
    19681 cctggcagaa taattccctg cgggccgccg tcgacttcac ccggtacttc cagcccgtgc
    19741 cagtgcaacg tcgtctcttc cgtcagttgg ttatagatat caaccgttac cgctttgccg
    19801 cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt agttgcagtt
    19861 ttctcgccaa aggtagactg acctgcgcca atagttaact gaatgcgatt acgggcatcg
    19921 gtcgtgagca aatcagggat cggtaacgtt gggcgttccg ccgcaaagac tgcgcggctc
    19981 cacagcggca aggctgaagc cacacccagc gcgacggaat attttaagaa atcacgacgt
    20041 tgcatagtta tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg agcatagtcc
    20101 ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat ggtcgtcgcc
    20161 tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca atgaagacgt
    20221 ttttcagaac agtgttattc ggcagcctga tggccgtttg cgcaaacagt tacgcgctca
    20281 gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt ctgaagaacg
    20341 attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc tttttcgccc
    20401 agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagcc ctcggtgaag
    20461 acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaagtgc aaagccctgg
    20521 cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct ttttgtagaa
    20581 atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt ttttacgggt
    20641 gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa acgctacacg
    20701 accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag accgatcacc
    20761 aggatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg gatggcgtag
    20821 tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat gttccgctac
    20881 tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt gccatgctgc
    20941 gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc gatgcgggtg
    21001 tcgtgtcgtt ctgccgtcag tatctgccca accataacgc cggtagctac gacgatccgc
    21061 gctttaagct ggtgatcgac gatggcgtca atttcgttaa tcaaaccagc cagacctttg
    21121 atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagtctg ttcacctcag
    21181 cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc gcacaaaacg
    21241 gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc agccattact
    21301 tcagcgacgt tggcttttat caggcggcga tcccgaccta ttacggcggt atcatgactt
    21361 ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt attcaggcgc
    21421 gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcaatccat acggcagctt
    21481 ttgccttacc tcagtatctg caagacgcac tggcttcaca gccgtcctaa ggagaagata
    21541 agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt ctgagttttt
    21601 gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat attgcttata
    21661 tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc tgttccatta
    21721 tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt gccagcgtca
    21781 ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca gaacaccccg
    21841 gcccactgcc agaaacggtc gttgcccatc tcgataaaag tcatatttgc gtacatacct
    21901 acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt gaagtctcta
    21961 cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag cttgagtccg
    22021 atatcgtaac cattgattat cgcgtgcgcg gttttacccg cgacattaac ggtatgaagc
    22081 actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat atgaaggcgc
    22141 tgtatgacat ggtggatgtg aacgtctatc aggaaaatat cttccatacc aagatgttgc
    22201 ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat ttaaccgaca
    22261 gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt tattacgggc
    22321 gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca gatttattta
    22381 cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct caacgccgca
    22441 tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat cgccagcgaa
    22501 ttccagttga ttggcgcgaa ccatcacctc ttcaccgtcc atccacaggg tgtattcatg
    22561 ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt gttccacttc
    22621 atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga ccacttcatg
    22681 ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt cgtaatccat
    22741 agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat tgcgccattg atgtaattat
    22801 cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa aaatgcccgg
    22861 gaatctcatt caccgggcat tgtgtcgttt atgcgcagcg cgtgcgctta cttattacac
    22921 cgcagtctgg aaaatcaccc catcggcttt ttcggtgtac tgagaaagct ggttgaagtt
    22981 cagataacga taagtatcaa cggcagtttt atctacttgc gcaacgtagg tctggtactc
    23041 ttccggcgtc ggcagtttgc caatcagcgc cgcaacagcc gccaattctg cagaagccag
    23101 gaagacattc gcgccagtcc ccagacggtt cgggaagtta cgggtagagg tggaaaccac
    23161 cgtcgcaccg tctgctacac gcgcctggtt acccatacac agggaacagc cagggatctc
    23221 gatacgcgca ccgctcttac cgaagacgct atagtagcct tcttcggtca actgcgcggc
    23281 atccatacgg gttggcggtg ccacccacag gcgcgtcggc aactggcctt tgtgcgcatc
    23341 cagcagttta cccgcagcac ggaagtgacc gatgttggtc atgcaggaac cgataaacac
    23401 ttcgtcgatc ttctcgccct gtaccgcaga cagcggacgt gcgtcgtccg ggtcgttcgg
    23461 tgcacagagg attggctctt taatatccgc cagatcgatg tcgatcactg ccgcgtattc
    23521 cgcatctgca tcggcttcca gcagctcagg attcgccagc catttttcca tgccctgaat
    23581 acgacgttcc agggtacgac gatcgccgta accttccgcg atcatccact tcagcaggac
    23641 gatgttagag ttcaggtatt cgatgatcgg ttctttgttc agcttgatgg tacaaccagc
    23701 ggcagaacgc tcggcggacg catcggtcag ttcaaatgcc tgctcaactt tcagatccgg
    23761 cagaccttca atttccagaa tgcgaccaga gaagatgttt ttcttgcctt tcttctcaac
    23821 ggtcagcaga ccttgtttga tcgcatacag cgggatcgcg tgtaccagat cgcgcagggt
    23881 gatgcccggc tgcattttgc ctttgaagcg caccagaacg gattccggca tatccagcgg
    23941 cattacgcct gttgcggctg caaatgccac cagaccagaa cccgccggga aagagatacc
    24001 gatcgggaaa cgggtatggg agtcaccacc ggtaccgacg gtatccggca gcagcatacg
    24061 gttcagccag gagtggatta cgccgtcacc cggacgcagc gacacaccac cacggttcat
    24121 aatgaagtcc ggcagcgtgt ggtgcgtgtt cacgtcaact ggcttcggat acgccgcggt
    24181 atggcagaaa gactgcatca ccaggtcagc cgagaagccc aggcacgcca ggtctttcag
    24241 ttcatcacgg gtcatcgggc cggtggtgtc ctgtgaacct acagaagtca ttttcggttc
    24301 gcagtacgcg cccggacgaa tacctttcac accgcaggca cgacctacca ttttctgcgc
    24361 cagcgagaag ccgcgatcgc tctcagcgac atctttcgcc tgacggaaca catcactgtg
    24421 cggcagacca agtgcttcac gcgctttggt ggtcaggcca cgcccgataa tcagcgggat
    24481 acggccgcca gcacgcactt catcaatcag cacgtcggtt ttcagttcga aggtcgccag
    24541 cagttcgccg gtttcgtggt tacgcacttc acctttgtac gggtaaacgt caatcacgtc
    24601 gcccatgttc aggttagaga cgtcgacttc gattggcagt gcacccgcgt cttccatcgt
    24661 gttaaagaag atgggtgcaa ttttaccgcc gaggcacaaa ccaccgccgc gtttgttcgg
    24721 cacatgtgga atatcatcgc ccataaacca cagaacggag ttcgtggcgg atttacgcga
    24781 tgaacccgta cccacaacgt caccgacgta cgccagcggg aaacctttct gttgcagagc
    24841 ttcaatctgt ttgatcgggc caacaacacc tggctggtct ggctcaatac cttcacgggc
    24901 gtttttcagc atcgccagcg cgtgcagtgg gatatccggg cgcgaccacg catccggtgc
    24961 cggagaaagg tcatcggtgt tagtttcgcc agtgactttg aagacagtaa cggtcagttt
    25021 ttcagccagc gccgggcgat tcaggaacca ttcggcatcc gcccaggact gcataacctg
    25081 cttcgcatat tcgttgcctg ctttcgcttt ctcttctacg tcatagaagt tatcgaacat
    25141 cagcagcgtg tgagaaagtg ctttggcagc aataggtgcc agtttggcat catccagcgc
    25201 gtcgatcagc gaatgaatgt tgtaaccacc ctgcatggtg cccagcagtt cgatggcttt
    25261 ttccggagtc agcagagggg atttggcttc gcctttcgcg atagcagcca ggaagcctgc
    25321 tttgacatag gcggcttcat cgacgcctgg gggaacacgg ttggttaaca gatctaacag
    25381 gaattcttct tcgcccgcgg gcgggttttt cagcagctct acaagtgcgg ccatttggtt
    25441 tgcatccagg ggtttgggcg caatcccctc agcggcacgc tcagctacgt gcttacggta
    25501 ttcttctagc acgacggttc tcctcgctct cattgtcata gtgcggcagg cgattctctt
    25561 cacgctcctg tgagacagca gtttgtaggg taaatgccca gtaccgcaac gggcagaata
    25621 gcaggatttt gacgatgtgt taatctgttt acaaaaaagc aacataaaaa agtggctgaa
    25681 tcgttaagga tggtctagag attgtttccc tccgtaaaaa cttcgttaat tcgcatggtg
    25741 ataatcacct ttctcaacga atcccaagat tagtcaaaat ttaaactacc gcctctttat
    25801 actcggattc acagcacctg cgggtggcag ttcgccgacc attgcgattt ccttgagatc
    25861 cgaattatga aaatgacttt gccgtttaaa ccccatgtgc tggcgctaat ttgcagtgcc
    25921 gggctttgtg ccgcctctac cgggctatat ataaaaagcc gcacagtgga agcgcctgtg
    25981 gaaccgcaat cgacacaaca gactgcgcct gacatcaccg cagttacgct tcctgcaacg
    26041 gtttccgcac cgcccgtaac gcccgccgtc gtcaaatccg cattcagcac tgcacaaatc
    26101 gatcaatggg ttgcgcctgt cgcgctgtac cccgattctc tgctttcaca agtgttaatg
    26161 gcatcaacct atccggcaaa cgttgctcaa gcagtgcaat ggtcgcacga taatccactt
    26221 aaacaaggcg atgctgctat tcaggcggta tctgaccagc cgtgggacgc cagcgttaaa
    26281 tcactggtgg cctttccaca attgatggca ttgatgggcg aaaacccgca atgggtgcaa
    26341 aacctgggcg atgcttttct ggcgcagccg caggacgtga tggactcggt acagcgattg
    26401 cggcaactgg cgcaacaaac cggttcgctg aagtcatcaa ccgaacaaaa agttattacc
    26461 acaacgaaga aagctgtacc ggtaaaacag acagtcacgg cacccgtcat accatccaat
    26521 accgttttaa ctgccagccc cgtcattaca gagcctgcaa caaccgtcat ttccattgag
    26581 cccgccaatc ctgatgtggt ctatattccc aactacaacc caaccgtggt ttacgggaac
    26641 tgggccaata ctgcgtatcc gccggtttat ctgccaccac cagccggaga accgtttatt
    26701 gacagctttg tgcgcggatt cggctatagc atgggtgttg ctaccacgta cgcactattc
    26761 agcagcatcg actgggatga cgacgatcat gaccatcatc atcatgacga tgatgattat
    26821 catcaccacg atggcggtca tcgtgacggt aatggctggc aacacaacgg cgacaacatc
    26881 aatatcgacg tcaacaattt caaccgtatc accggtgagc atcttactga taagaatatg
    26941 gcatggcggc acaatccaaa ctaccgtaat ggtgtgccct atcatgatca ggatatggca
    27001 aagcggtttc atcaaaccga tgtcaacggc ggaatgagcg ccacgcagct acctgctcca
    27061 acacgcgaca gccagcgtca ggcggcagca agtcagtttc agcaacgaac acacgccgcc
    27121 cccgtcatta cacgagatac ccaacgtcag gcagcggcac agcggtttaa tgaagctgaa
    27181 aactatggga gctatgacga cttccgcgac ttcagccgtc gccaaccact gacccagcaa
    27241 caaaaggacg ccgctcgtca gcgttatcag tcggcctcgc ctgagcagcg ccaggcagtc
    27301 cgcgagaaaa tgcagactaa cccacagatc cagcagcgaa gagacgcagc gcgtgagcgt
    27361 attcagtcag cctcgcctga gcagcgccag gtgtttaagg aaaaagtaca gcagcgccca
    27421 ctgaaccaac agcaacgtga taacgcccgc cagcgtgttc aatcagcatc acctgaacaa
    27481 cgtcaggttt ttcgggaaaa agttcaggag agccgcccac aacgtctaaa cgacagtaac
    27541 catactgtca ggctgaataa cgagcaacgg tcagcagtac gcgaacgtct ctctgagcgc
    27601 ggagcaaggc gacaggaaag gtaaattgca gacgtaaaaa aagcggcgtg gttagccgct
    27661 tttttaattg ccggatgttc cggcaaacga acaattactt cttcttcgct ttcgggttcg
    27721 gcaggtcggt aatgctacct tcgaacactt ctgccgccag gcccacagac tcgtgcagag
    27781 tcgggtgcgc gtggatggtc agtgcgatgt cttcagcatc acaacccatt tcgattgcca
    27841 ggccgatttc acccagcagc tcgccgccgt tggtaccgac aatcgcacca ccgatcacac
    27901 ggtgagattc tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtcg gaagcgatag
    27961 cacgaccaga agcagcccac gggaaggtgg cggtttcata gctgatgcct ttctctttcg
    28021 cttctttctc agtcagacct acccatgcaa cttctggttc ggtataggcg atggacggga
    28081 taactttcgg atcgaagtag tgtttcttac cggcgataac ttcagcggca acgtgacctt
    28141 cgtgaacacc tttgtgtgcc agcatcggct gaccgacgat atcgccgata gcaaagatgt
    28201 gcggtacgtt ggtacgcagc tgtttgtcaa cgcggatgaa accacggtcg tccacttcca
    28261 cgccagcttt gcctgcgtcg aggtttttac cgttcggcac acgaccaatc gctaccagca
    28321 cggcgtcgta acgctgcggt tcagcgggtg cttttttgcc ttccatcgtc acataaatac
    28381 cgtcttcttt cgcttcaacg gcggtaactt tggtttccag catcaggttg aatttcttgc
    28441 tgatacgctt ggtgaagact ttaacgatgt ctttgtcagc tgccgggata acctggtcga
    28501 acatttcaac cacgtcaatc tgtgaaccca gcgcgtggta tacggtgccc atttccagac
    28561 cgatgatacc gccacccatt accagcaggc gttctggtac ttctttcagt tccagcgcgt
    28621 cagtggagtc ccagatacgc ggatcttcat gcggaataaa cggcagttgg atcgggcgag
    28681 aacccgctgc aatgatcgca ttgtcgaagt tgatcacggt tttgccgttc tcaccttcaa
    28741 cttccagggt gttagccccg gtgaatttac ccagaccgtt gaccactttg actttgcggc
    28801 ctttcgccat accagccaga ccaccggtca gctgattgat cactttctct ttccaggtac
    28861 gaatcttgtc aatatcggtt ttcggttcgc cgaagacgat accgtgttca gccagcgctt
    28921 tggcttcttc gataactttt gctacgtgca gcagtgcttt agaagggata cagccgacgt
    28981 tcaggcaaac accgccaagg gtgttgtaac gttctacgat tacggtttcc agacctaaat
    29041 cagcgcaacg gaaggcagcg gagtaacctg cggggcctgc cccaagtacc acgacctgag
    29101 ttttgatttc agtactcatc atgacctcta tatatttatc tccggcggtc atacccgtcg
    29161 tctttcaggc cgcagcggcg ttagcttcac gccctcaccc cagtcactta cttatgtaag
    29221 ctcctgggga ttctggcgct tgctaccttg ctgcacccag aaatccatag ggtatgtgaa
    29281 gtaataccct aaccaccacc gggtcgttct atccgtcggt attttacaaa attgttaaca
    29341 atttttaaac aacaaacggc aaccgatttg tctattcgct aataacctca atacattcat
    29401 gagattacca gaaaaaagcc ggccgttggg ccggctcttt tacttacatc accagacggc
    29461 gaatgtcaga cagcgtgttg ttaatgatgg taatgaaacg ggcaccatca gcaccgtcga
    29521 tcacgcggtg gtcgaaggag agagaaatcg gcagcatcag acgcggcacg aactctttac
    29581 cattccacac cggctccatc gcggacttgg aaacgccgag gatagccact tccggcgcgt
    29641 tcacaatcgg cgcgaagtgg gtagtaccca ggccgccgat gctggagatg gtgaagcaac
    29701 cgccctgcat ttcgcccgca gtcagcttac cgtcacgcgc tttcttagaa atagtcatca
    29761 gctcgcgaga cagctcgatg atgcctttct tgttgacgtc tttgaatacc ggaacaacca
    29821 gaccgttcgg ggtatccacc gccacaccga tgttgatgta tttcttcagg gtcagacgct
    29881 gaccgtcttc cgacaacgaa ctgttgaagc gaggcatctg ctcaagagct gcagcaacgg
    29941 ctttcatgat gaagacaacc ggggtgatct tcacatccag cttacgtttc gccgcttctt
    30001 cgttctgctg tttacggaac gcttccagct cggtgatatc ggttttgtcg aagtgagtaa
    30061 catgcgggat catcacccag ttacggctca ggttcgcacc agaaattttc tggatacggc
    30121 ccagttccac ttcttcgatt tcaccaaact tgctgaagtc caccttcggc cacggcagca
    30181 tgcctgggat accgccgcca gtcgccgccg gagctgcttc tgcacgtttg atagcttctt
    30241 tcacgtaagc ctgaacgtct tcgcgcagga tacgaccttt acggccagtg cccttcactt
    30301 tcgccaggtt aacgccaaac tcgcgtgcca gacggcggat cagcggagtc gcgtgaacgt
    30361 aagcgtcgtt ttctgcaaat tcagatttgc cttccgcttt cgcagccggt gctgctgccg
    30421 gagcttcagc ttttgctgcc ggagccggcg ctgccgcttc ctgtttcgca ggagccgccg
    30481 caggcgctgc gccttcaact tcgaagatca taatcagcga gccagttttc actttatcgc
    30541 caacgttgac tttcagttcc ttcacgacgc ctgcaaacgg agccggaact tccatagaag
    30601 ctttgtcgcc ttctacggta atcagtgact gttcagcggc aactttgtcg cccactttca
    30661 ccatcacttc ggtcacttca acttcgtcac cgccgatatc cggaacgtta acttctttca
    30721 cgccagccgc tggtgcaggg gccgctgccg gagccgcttc ctgtttagcc gccggagctg
    30781 ccgcgcctgc ttcacccgcg acttcgaaga ccataatcag cgagccggta gacactttgt
    30841 cacccacgtt cactttgatc tctttcacgg tgccagcaaa cggagccgga acttccatag
    30901 acgctttatc gccttctacg gtgatcagcg actgttcagc ttcaacttta tcgccaactt
    30961 tcaccaggat ttcggtcact tcaacttcgt cgctgccgat atccggaacg ttaacgtctt
    31021 ttgccgctgc agccgctggt gctgctgccg gagctgcttc tttcttctct tctgcctgag
    31081 caggtgcagc gtctgctgca ccgtcggcgg aatcgaaaat cataatcagt gcgccggtct
    31141 gggttttatc gccaacagag actttgatct ctttaacgat acccgcctgc ggagacggaa
    31201 cttccataga ggctttgtcg ccttctacgg tgatcagcga ctgttcggct tcaactttgt
    31261 cgcccacttt gaccaggatc tcggtgattt caacttcatc agccccgatg tccggtactt
    31321 tgatttcgat agccattatt cttttacctc ttacgccaga cgcgggttaa ctttatctgc
    31381 atcgatgttg aatttggcga ttgcgtcagc aaccactttc ttatcgattt cgccacgttt
    31441 agccagttcg cccagcgccg ctacaaccac gtaggaagca tcaacttcga agtggtgacg
    31501 caggttctca cggctgtcgg aacgaccgaa gccatcagta cccagtacgc ggtagtcgtc
    31561 agccggtacg taagtacgga cctgctcagc gaacagtttc atatagtcgg tagatgccac
    31621 tgccggagcg tcgttcatca cctgagcgat atacggaacg cgcggagttt ccagcgggtg
    31681 cagcatgttc cagcgttcac aatcctgacc atctcgcgcc agttcggtga aggaggtcac
    31741 gctataaacg tcagaaccta cgccgtaatc tttcgccagg atctctgccg cttcacggac
    31801 gtgacgcagg atagaaccgg agcccagcag ctgaacttta cctttgctac cttcaatggt
    31861 ttcgagtttg tagatacctt tacggatacc ttcctcagca ccttccggca ttgccggcat
    31921 gtggtagttt tcgttcagcg tggtgatgta gtagtaaacg ttctcttgtt tttcaccgta
    31981 catacgctcc agaccgtcat gcataatgac agcaacttcg taagcgtaag ccgggtcgta
    32041 agagatacag ttcggaatag tcagcgactg aatgtggctg tgaccatctt cgtgctgcag
    32101 accttcgccg ttcagggtgg tacgaccgga agtaccgccg atcaggaagc cacgcgcttg
    32161 ctggtcgcca gccgcccagc acagatcgcc aatacgctgg aagccgaaca tcgagtaata
    32221 gatgtagaac gggatcatcg gcagattgtt ggtgctgtaa gaggtcgccg ctgccagcca
    32281 ggaacaacct gcgcccagct cgttgatccc ttcctgcaga atctgacctt tctcgtcttc
    32341 tttatagtaa gcaacctgct cgcggtcctg cggggtgtac tgctgaccgt tcgggctgta
    32401 aataccaatc tgacggaaca gaccttccat accgaaagta cgcgcttcgt cggcgatgat
    32461 cggtaccaga cgatctttga tcgacttgtt cttcagcatt acgttcaggg cacgaacgaa
    32521 agcgatagtg gtagagatct ctttgctctg ctcttccagc agcgcgccga agtcttgcag
    32581 gctcggcagc tcaagcttct cggtgaagtt cggctgacgg cttggcagat aaccgtgcag
    32641 tttctgacgc tgagcgtgca gataggtatg ctcttcagaa ccttccggga aggtgatgta
    32701 cggcagtttt tcgatatctg catcagacac cggcacattg aaacggtcgc ggatgtgacg
    32761 cacgccgtcc atgttcattt tcttaacctg gtgcgcgatg tttttacctt cagccgcgtc
    32821 gcccatgccg taacctttaa tggtatgagc aaggattact gtcgctttgc ctttggtttc
    32881 ctgcgctttc ttgaatgcag cgtagatttt cttcggatcg tgaccaccac ggttcagtgc
    32941 ccagatctgc tcgtcagtcc agtctgcaac cagtgctgcg gtttcaggat atttaccgaa
    33001 gaagtgttca cgaacgtacg caccatcttt cgatttgaag gtctggtagt cgccgtcaac
    33061 ggtttcgttc atcagctgga tcagtttacc gctggtatct ttacgcagca gttcatccca
    33121 acggctaccc cacatcactt tgatcacgtt ccagccagca ccttcgaaga tgccttccag
    33181 ttcgttgatg atcttgccgt taccggtgac cgggccgtca agacgctgca ggttacagtt
    33241 gataacgaag actaggttat ccagtttttc acgggtagcg atggtgatcg cacctttgga
    33301 ttccggttcg tccatttcac cgtcgccgag gaacgcgtaa acggtctgtt tagaggtatc
    33361 tttcaggcca cggtgttcca gatatttcag gaatttagcc tggtaaatag caccaatcgg
    33421 ccccagaccc atagatacgg tcgggaactg ccagaattcc ggcatcagtt tcgggtgcgg
    33481 ataggaagag aggccattgc cgtgaacttc ctgacggaag ttatccagct gctcctgagt
    33541 cagacgacct tctaggaaag cacgagcgta cacgcccggg gagatgtggc cctggaagta
    33601 aaccaggtcg ccgccatcct gctcgttgcg tgcacggaag aagtggttaa agcacacatc
    33661 ataaatggtt gcggaagact ggaaggacgc catatggccg cccagttcga ggtctttttt
    33721 cgacgcacgc agcaccgtca tgatggcgtt ccagcggata gctgaacgaa tacggcgttc
    33781 cagttccaga ttacccggat actccggttg ttcttcaacg gggatggtgt tgatgtagtt
    33841 gctgatacct gtgcctgcgg ctacgtttac accgcctttg cgggcttcag caagcagttg
    33901 gtcgatcaga tactgagcac gctcaacacc ttcttcacgg atgaccgatt cgatcgcctg
    33961 gagccagtcg cgagtttcga tcggatccac gtcatttggg aaacgttctg acatgggtta
    34021 ttccttatct atctaataac gttgagtttt ctggaacctg tcccattgaa ctctcgccgg
    34081 aaagctcaat aagacaggtt ctacgtttag ttgccgcgct tttatatgcg cttgatttac
    34141 aacatcttct ggataatttt taccagaaaa atcactaatt ctttcgttgc tccagacgac
    34201 gcagagaacg ctcacggcgg ctctcttcac gacttctgtc gagcaaaatt tcttcgataa
    34261 aggccagatg gcgatgcgat gcttcgcgcg cttcttccgg cttaccggcc ataatcgctt
    34321 caaatatgcg ggtgcggtga ctactcacca gcggcagcat ctcgcgacgc gaatagagca
    34381 attcgaagtt ctggcggaca ttctgggcca acatcggctc catacacctt agcagatgaa
    34441 gcagaaccac attgtgggcc gcttcagtga cagcaatctg atactggagt acggcgtttg
    34501 attccgcgtc cagatcgcca gactgctgcg ccagctctat ggcgtggtgg agttcacgga
    34561 tgcgttcctt gtcttcatcg gtactacgca gcgcggcgta ataagcggcg ataccttcca
    34621 gggcgtgtcg tgtttcgagc aagtcatact gtgactcagg atggtcggag agcagctcca
    34681 ccagcggatc gctgaagctt tgccataggc tgctctggac aaaagtgccg ccaccctggc
    34741 gacgaagcaa caagcccttc gcttcgagac gttgaatcgc ctcacgcaag gagggacggg
    34801 agacgtcaaa ctgttttgcc agttcgcgtt ccggtgggag tttttcgccc gggcggagag
    34861 tgccttcgag gatcaaaaac tccagttgct gctcaatcac atcggagagt tttggttggc
    34921 ggattttgct gtaggccatg agttcctgtc ttaagccact tgccgaagtc aattggtctt
    34981 accaatttca tgtctgtgac gctaaagtaa caaagtattc accttatgtc catacaggtt
    35041 ttgattgaaa tcatgaaact gtgcacattt taacaacttg acatatataa cgtttcaaag
    35101 ttgtaactat gcacaaatgt agactttacg taggaaagga gttttttaac gattattaaa
    35161 ctataaaaat ccgaattgaa ccgattcact taccaatttt gtgattttta attcaattaa
    35221 aacgaattta aattcattct acatattgag aggggttgag gctgagcttt acaaacggtt
    35281 tctttttaag caactcatct tcaaccatgc ataaagcggg tgcattcgct gcctcatacc
    35341 attattcttg atctgacgga agtctttttg taacaattca aacttctttg atgtaaacaa
    35401 attaatacaa caaacggaat tgcaaactta cacacgcatc actgcgtaga tcaaaaaaac
    35461 aaccaccgca cgaggtttca tgatggaagg tcaacagcac ggcgagcagc taaagcgcgg
    35521 ccttaaaaac cgccatattc agcttatcgc gctgggtggc gcgataggga ccgggttatt
    35581 cctgggtagc gcctccgtaa tacagtccgc agggccaggg attatcctgg gttacgccat
    35641 tgctggtttt atcgcctttc tgatcatgcg tcagctgggt gaaatggtgg tcgaagaacc
    35701 tgtcgcaggc tcctttagcc actttgctta taaatactgg ggcagttttg ccggtttcgc
    35761 ctctggctgg aactactggg tactgtacgt tttagttgcc atggctgagc tgactgccgt
    35821 gggtaaatac attcagttct ggtatccgga aatccccacc tgggtttctg ccgccgtatt
    35881 ctttgtggtg attaacgcca tcaacctgac caacgttaaa gtgtttggcg agatggagtt
    35941 ctggtttgcc attatcaaag ttatcgcggt ggtagcgatg atcatcttcg gcggctggct
    36001 gctattcagt ggcaacggcg gcccgcaggc gaccgttagc aacctgtggg atcagggtgg
    36061 tttcctgccg cacggcttca ccgggctggt gatgatgatg gcgatgatca tgttctcgtt
    36121 cggtggtctg gaactggtgg ggatcaccgc agcagaagct gataacccgg agcaaagtat
    36181 accgaaagca actaaccagg ttatctaccg catcctgatt ttctatattg gttcgttagc
    36241 cgttctgctc tcactgatgc cgtggacccg cgttaccgcc gataccagtc cgtttgtgct
    36301 gatcttccac gagttaggcg atacctttgt ggcgaatgcg ctgaacatcg tggtactgac
    36361 tgcggcgctc tccgtgtaca acagctgcgt atattgcaac agccgtatgc tgtttggtct
    36421 ggcacaacag ggtaatgcgc caaaagcgct ggcgtctgtc gataaacgtg gtgtaccagt
    36481 aaataccatt ctggtgtctg cactggtaac ggcgttgtgc gtactgatta actaccttgc
    36541 cccagagtcc gctttcggac tgttaatggc gctggtggta tctgcactgg taatcaactg
    36601 ggcgatgatt agcctggcgc atatgaaatt ccgtcgcgcc aagcaggaac aaggcgtggt
    36661 aactcgcttc cctgctctgc tttatccgct gggtaactgg atctgcctgc tgtttatggc
    36721 ggcggtactg gtgattatgc tgatgacccc aggaatggcg atttcggtat acctgatccc
    36781 ggtatggctg atcgtgttag gtatcggcta tctgtttaaa gagaaaaccg ccaaagccgt
    36841 aaaagcgcat taatctctct acgccctcac ccgtacaggg tgagggcaat aatctttaca
    36901 ccaacgcccc gtaaatcgtc agtagtgcaa tcaccaccac gaccacgaac gaggttttct
    36961 tcgccattga aaccgctgcc ctcggcgtct ccaccttatc gacatgcggt tcacgcgcca
    37021 gagagaactg cgccagacgc gttaacacct gatactgcga agtatggaaa tcacccagcg
    37081 aagcaaacca ggccggtaac gctttctcac catgaccgat caaggcatat accacacccg
    37141 caagacgaac cggcacccaa tccagtacat gaagcacggc atcaatgccg gactgtaaac
    37201 gatgatgcgg cgtctgatat cgcgccagcc agtattgcca tgcacgcaaa aacgcatacc
    37261 ccatcagcgt aacgggtccc caggttcccc ccacaatcag ccagaacagc ggtgcaagat
    37321 aaaaacgaaa gttaatccac agcaatgcat tttgcagctc acgcaaatac tcacgttcgt
    37381 cgcagcctgc cgggacgccg tgaatcatgg tgagttcgcc agccatcgtg gcacgggcat
    37441 ggctatcatt acgtgaagca gctgtcagat aagcatgata atgaagacga actttacctg
    37501 cgccaataca cagcaaacca atcagcagcc acaccagtag cgtgggaacg ttgaacaata
    37561 ctccctgcaa tgcgcgtaac agtaaaaaag tcacgcccat cgcaataatg gtcatgccta
    37621 acgtgcgccc gagagaaaaa tgtttcaccc gccgaaagaa cgcttcaaga cgatgatcaa
    37681 gctgccagtg ctcgcccaac ttaaacaggc gctcgaaaat taacaccagt aaggttgtaa
    37741 atagcgtcat gttgtctcct tgctgaccag cacacgaaac cgtgcccaat caaatgcagg
    37801 accgggatcg gttttccgat ccggcgcaat atcacaatgg cccgtcatgt ttttagcgat
    37861 atccggatag caatcaatca gtgcccgcgt aaccgccgca agctgttgat actgcgcatc
    37921 ggtatacgcc agcgtatcgg tgccttcaag ctcaatccca atagaaaaat cattgcagcg
    37981 ttcgcgcccc tgatactgag agactcccgc atgccatgca cgtttatcga aaggaacata
    38041 ctggactatt tcaccatcac ggcgaatcaa acagtgagcg gagacgcgca aatgggcgat
    38101 ctcagcaaag aaaggatgtg cctgcggatc aatagttcca gtgaataatg cgtcgatcca
    38161 cggaccgcca aactcgcctg gcggcaggct aatattgtgc accaccagca gggtgggtgt
    38221 ttcgtcatcc gggcggcaat cgtaatgtgg tgagggaacg cggcgcgcgc caaccagcca
    38281 cccctgttct aacaacatgc aggatctcct tatatgtggt gctaataccc ggttcagagt
    38341 agcatgtttc taccttatga ttcgttagct atctggagtt ttaacatgcc gcctcgccgc
    38401 tataaccctg acacccgacg tgacgagctg ctggaacgca ttaatctcga tatccccggc
    38461 gcggtagccc aggcgctgcg ggaagattta ggcggaacag tcgatgccaa caatgatatt
    38521 acggcaaaac ttttaccgga aaattctcgc tctcatgcca cggtgatcac ccgcgagaat
    38581 ggcgtctttt gcggcaaacg ctgggttgaa gaggtgttta ttcaactggc aggcgacgat
    38641 gtcaccataa tctggcatgt ggatgacggc gatgtcatca atgccaatca atccttgttc
    38701 gaacttgaag gcccatcccg cgtgctgtta acgggcgaac gcactgcgct taattttgtg
    38761 caaacccttt caggagttgc cagtaaggta cgccactatg tcgaattgct ggaaggcacc
    38821 aacacgcagt tgttggatac gcgcaaaacc ttacccggcc tgcgttcagc tctgaaatac
    38881 gcggtacttt gcggcggcgg agcgaatcac cgtctggggc tttctgatgc cttcctgatc
    38941 aaagaaaacc atattattgc ctccggctca gtgcgccagg cggtcgaaaa agcgtcctgg
    39001 ctgcacccgg atgcgccagt agaagtcgaa gtagagaatc tggaagaact tgatgaagcc
    39061 ctgaaagcag gagccgatat catcatgctg gataacttcg aaacagaaca gatgcgcgaa
    39121 gccgtcaaac gcaccaacgg caaggcgcta ctggaagtgt ctggcaacgt cactgacaaa
    39181 acactgcgtg aatttgccga aacgggcgtg gactttatct ccgtcggtgc gctaactaaa
    39241 cacgtacaag cactcgacct ttcaatgcgt tttcgctaat agttgacaga tttatccgct
    39301 ccatcgcggg cggataatct ccccttcccc aactttcttc gtaacgcctc gcaaatttct
    39361 tgtctttcgc tgcaacgaaa taaaaaacct cggaatctgc tttcccgttt cgttttttgc
    39421 ccctcgcctg tgctgatggc gtttgccaaa gtagcgccaa ctaaatcaag gagcgaaaca
    39481 gatggacaag caacgcggtt ttacacttat cgaactgatg gtggttattg gcatcattgc
    39541 cattttaagc gccattggta ttcccgctta tcaaaactac ctgcgcaaag ccgcactcac
    39601 cgacatgcta caaacctttg tgccttaccg taccgccgta gagttgtgcg cgctggaaca
    39661 tggtggatta gatacctgcg acggtggcag caatggcatt ccctcgccta ccaccacccg
    39721 ctatgtttca gccatgagtg tggcaaaggg cgtggtgtcg ctgaccgggc aagaaagtct
    39781 caatgggcta agcgtcgtca tgacaccggg ttgggataac gcaaacggcg tcaccggctg
    39841 gacgcgcaac tgcaatattc aaagtgacag cgcattgcat caagcctgcg aagatgtctt
    39901 ccgctttgat gacgccaact aaggagcggc aatgaatatt ccacagctca ctgccctgtg
    39961 tctgcgttat catggagtct tgctggatgc cagcgaagag gtggttcatg ttgcggtagt
    40021 cgatgcacct tcgcatgagc tactggacgc attgcatttc gctaccacca aacgtattga
    40081 gatcacctgc tggacgcgcc aacaaatgga aggtcacgcc agtcgcacac aacagacatt
    40141 gcccgtagct gttcaggaga agcatcagcc caaagcagag ttgctaactc gaacgttaca
    40201 atctgcgctg gaacaacgcg cgtctgatat tcatatcgaa ccagcggaca atgcctaccg
    40261 catccgcttg cgtatcgacg gcgtattgca tcctttaccg gatgtttcac cggatgccgg
    40321 agtcgcatta accgccagat taaaagtgct gggaaacctg gatattgcgg aacatcgcct
    40381 gccgcaggac gggcaattca ctgtcgaact ggcaggaaac gccgtctcat ttcgtattgc
    40441 gaccttacca tgtcggggtg gtgaaaaggt ggtattaagg ttgttacagc aggtgagcca
    40501 ggcactggat gtcaacacgc ttggaatgca gccgttacaa ctggcggact ttgctcatgc
    40561 cttgcaacaa ccacagggac tggtgctggt aactggccct acaggcagcg gcaaaacggt
    40621 cacgctttat agtgccctgc aaacgctgaa taccgctgac attaatattt gtagcgtcga
    40681 agatccggtt gagatcccca tagccggact aaaccagacg caaatccatc cgcgtgccgg
    40741 actcaccttt cagggcgtgt tgcgtgcgtt attgcgccag gatcctgacg tcatcatgat
    40801 cggagagatc cgcgatggcg aaacagcaga gatcgctatt aaagcggcgc aaactggtca
    40861 cctggtgttg tctaccctac acactaattc cacctgcgaa acgctggtac gtttacagca
    40921 aatgggggtc gcccgctgga tgctatcatc ggcgcttacg ctggtaatag cccagcgtct
    40981 ggtacgcaaa ctttgcccac attgtcgccg gcagcaaggg gagcccatcc acattccaga
    41041 caatgtatgg ccatcgccgc tgccccactg gcaggcaccc ggttgtgtac attgctacca
    41101 cggtttttat ggtcgtacgg ccttatttga agttctgccc ataacgccgg tcattcgtca
    41161 gcttatttcc gctaataccg acgttgaatc gctggaaacg cacgcacgac aggcgggtat
    41221 gcgtacgctt tttgaaaacg gctgcctggc cgtggagcaa ggcttaacca cctttgaaga
    41281 gttaatccgc gtactgggga tgccgcatgg cgagtaagca actctggcgc tggcatggca
    41341 taaccggcga cggcaatgcg caagatggga tgctatgggc agagagccgt gctttgctgc
    41401 tcatggcact acagcaacag atggttaccc cacttagcct gaagcgaatc gccatcaatt
    41461 ctgcgcagtg gcgaggagat aaaagcgcgg aagtcattca tcaactggcg acgctactca
    41521 aagccgggtt aacgctttct gaagggctgg cactgctggc ggaacagcat cccagtaagc
    41581 aatggcaagc gttgctgcaa tcgctggcgc acgatctcga acagggcatt gctttttcca
    41641 atgccttatt accctggtca gaggtatttc cgccactcta tcaggcgatg atccgcacgg
    41701 gtgaactgac cggtaagctg gatgaatgct gctttgaact ggcgcgtcag caaaaagccc
    41761 agcgtcagtt gaccgacaaa gtgaaatcag cgttacgtta tcccatcatc attttagcga
    41821 tggcaatcat ggtggttgtg gcaatgctgc attttgttct gccggagttt gccgctatct
    41881 ataagacctt caacacccca ctaccggcac taacgcaggg gatcatgacg ctggcagact
    41941 ttagtggcga atggagctgg ctgctggtgt tgttcggctt tctgctggcg atagccaata
    42001 agttgctgat gcgccgaccg acctggctta tagtgcggca gaaattgctg ttacgcatcc
    42061 cgattatggg ttcactgatg cggggacaaa aactcacgca gatttttacg attctggcgc
    42121 tgacacaaag tgcaggcatt acttttttgc agggcgtaga gagcgtcaga gaaacaatgc
    42181 gctgcccgta ctgggtgcaa cttctgacac aaatccagca cgatatcagt aacggtcatc
    42241 ccatctggct ggcgctaaaa aataccgggg agtttagccc gctctgtttg caattagtga
    42301 gaacaggaga ggcatccggc tcgctggacc tcatgttaga caacctcgcc catcatcatc
    42361 gggataacac aatggcgctg gcggataacc tcgcagcctt actggaaccg gcgttgttga
    42421 tcataacggg aggaattatc ggtacgctgg tggtggcgat gtatctgcca attttccatt
    42481 taggcgatgc gatgagtggg atgggataac gctggcgtgt tgctccacac cagcgttgga
    42541 agattacagg ttgttgaaga tgcggttttc ttgttcctgc acacggataa atgtggtgcg
    42601 cttggtcagt tctttcaggc gtgaagcccc aacgtatgta caagctgaac gcaggccgcc
    42661 caaaatatca cgcgcggtat tttcaaccgg gcctcgcagc ggcagcttaa cggttttacc
    42721 ttctgctgcg cgatattccg caacgccgcc aacgtgacgt ttcatcgcag actcggagct
    42781 catgccgtag aacagcataa atttctcgcc gttctcctca acgatgcgac cgccgctctc
    42841 ttcgtggccc gccagcatgc cgccaagcat gacgaaatcg gcaccgccgc caaaggcttt
    42901 tgcgacatcg cccggcgtgg tgcagccacc gtcgctgacg atcattccgc ccagaccgtg
    42961 cgccgcatcg gcacattcga ttaccgcaga aagctgcgga taaccgacgc ctgttttgac
    43021 gcgagttgta caaacagaac ctgggccaat gccaacttta acgatatcag caccggagag
    43081 gataagctcc tcacacattt caccagtcac tacgttgcca gcacaaatgg ttttggtcgg
    43141 ccacgcttca cgcgctttcg caacgaactg cacgaagtgt tcggaataac cattcgccac
    43201 atcaatacaa acgaagttta atgccgggtt caggtcgaga atctgtttag ttttttcgaa
    43261 atccgcatca gacgtaccgg tagaaaccat cacatgtttc agcacatcag cggaagaatt
    43321 gttgataaac gcttgccact cttcgacaga atagtgttta tgcacagtag tcaaaatatc
    43381 aaaagaagcc agcgcagagg ccatagaaaa tgtacctacg gtgtccatat ttgcggcgat
    43441 aattggcacg ccggaccagc tctgacctga atgtttgaag gtgaattgac gttccagttc
    43501 aacatcggaa cggcttttaa gagtggagcg tttagggcgg atgagaacgt ctttaaaacc
    43561 taacttcaga tcttcttcaa tacgcatgtg cggattcctg gggttaatgg ctataagggt
    43621 aagagcgcaa ctccagtgac gctatcatac gcactaatca atgcggcgca agactgcgaa
    43681 attgtctttt tttacgctac aatcccataa atttacctgg taaatagtga gcgaacgttt
    43741 catcaatttc gctttcgtgc actcattttt aacgttttga taatcatgat gaattcccgg
    43801 gaatataaat tatgaggtat atagttgcct taacgggagg cattggcagt ggcaagagta
    43861 ccgttgccaa tgcgtttgct gatctcggaa ttaacgtcat tgatgccgat attattgcgc
    43921 gtcaggtggt tgaaccaggt gcacctgcgc tacatgccat tgctgatcac tttggcgcta
    43981 acatgattgc tgctgatgga acattgcagc gccgggcctt gcgcgagcgg atcttcgcca
    44041 acccggaaga gaaaaactgg cttaacgccc tgctgcatcc gctgattcag caagagacgc
    44101 aacaccagat ccagcaagct acttccccct atgtactgtg ggttgtgcca ttgctggtag
    44161 aaaactcact gtataaaaaa gcgaatcgag tgctggtggt ggatgtcagc ccagaaacgc
    44221 aacttaagcg caccatgcag cgcgatgatg taactcgcga gcatgtcgaa caaatccttg
    44281 ctgctcaggc aacgcgcgaa gcccgccttg ccgtggcaga tgacgtcatt gataataacg
    44341 gcgcaccgga tgctatcgca tcggatgttg cccgcctgca cgcacactat ttgcagcttg
    44401 cgtcgcagtt tgtctcacag gaaaaaccgt aatgcagacc caggtccttt ttgaacatcc
    44461 actaaatgaa aaaatgcgta catggctgcg cattgagttt ttgattcagc aactcaccgt
    44521 taatttaccc atcgttgacc acgctggcgc gctgcatttc ttccgtaatg tcagtgaatt
    44581 actggatgtt ttcgagcgcg gcgaagtccg cactgagctg ttgaaagaac ttgaccggca
    44641 gcaacgtaaa ctccagacct ggattggcgt gcctggcgtg gaccagagcc gtattgaagc
    44701 attaattcag cagttaaaag cggcggggag cgtattaatt tccgcgccgc gtatcgggca
    44761 atttctgcgt gaagatcgtt tgattgctct ggtgcgtcag cgactgagca tcccaggcgg
    44821 ctgttgcagc tttgatttac ctacattgca catttggctg catctacccc aggcgcagcg
    44881 cgacagccag gtagaaacct ggattgccag cctgaacccg ctcacccagg cacttaccat
    44941 ggtgctggat ttaattcgcc agtcggctcc cttccgtaaa caaaccagcc tgaatggttt
    45001 ttatcaggat aacggtggcg atgccgactt gctgcgcctg aatctgtcgc tcgattcaca
    45061 gctttatccg caaatttccg gacataagag ccgttttgcc attcgtttta tgccgctgga
    45121 cactgaaaac ggacaggtac cggaacgtct ggatttcgaa ctggcctgtt gctaaggagt
    45181 gaaaatgtca gaaactatta cggtgaattg cccaacctgc gggaaaacgg tggtgtgggg
    45241 tgaaatcagc ccgtttcggc cattttgctc caaacgttgt cagctgatcg acctcggaga
    45301 atgggctgct gaagaaaaac gaatccccag cagtggcgat ctttccgaaa gcgatgactg
    45361 gagcgaagag ccaaagcagt gacatttgtc tgatgcc
//
LOCUS       DAZFWL020000022        45188 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFWL020000022 DAZFWL020000000
VERSION     DAZFWL020000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 45188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.022"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(77..3439)
                     /gene="mscK"
                     /locus_tag="ACHKNP_RS06845"
                     /old_locus_tag="pgaptmp_001369"
     CDS             complement(77..3439)
                     /gene="mscK"
                     /locus_tag="ACHKNP_RS06845"
                     /old_locus_tag="pgaptmp_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HGU4703566.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(3567..4214)
                     /gene="acrR"
                     /locus_tag="ACHKNP_RS06850"
                     /old_locus_tag="pgaptmp_001370"
     CDS             complement(3567..4214)
                     /gene="acrR"
                     /locus_tag="ACHKNP_RS06850"
                     /old_locus_tag="pgaptmp_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HGU4703567.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQVKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            4356..5549
                     /gene="acrA"
                     /locus_tag="ACHKNP_RS06855"
                     /old_locus_tag="pgaptmp_001371"
     CDS             4356..5549
                     /gene="acrA"
                     /locus_tag="ACHKNP_RS06855"
                     /old_locus_tag="pgaptmp_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HGU4703568.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            5572..8721
                     /gene="acrB"
                     /locus_tag="ACHKNP_RS06860"
                     /old_locus_tag="pgaptmp_001372"
     CDS             5572..8721
                     /gene="acrB"
                     /locus_tag="ACHKNP_RS06860"
                     /old_locus_tag="pgaptmp_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HGU4703569.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDREKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            9267..9641
                     /gene="tomB"
                     /locus_tag="ACHKNP_RS06865"
                     /old_locus_tag="pgaptmp_001373"
     CDS             9267..9641
                     /gene="tomB"
                     /locus_tag="ACHKNP_RS06865"
                     /old_locus_tag="pgaptmp_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HGU4703570.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            9667..9885
                     /gene="hha"
                     /locus_tag="ACHKNP_RS06870"
                     /old_locus_tag="pgaptmp_001374"
     CDS             9667..9885
                     /gene="hha"
                     /locus_tag="ACHKNP_RS06870"
                     /old_locus_tag="pgaptmp_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HGU4703571.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            10057..10608
                     /gene="maa"
                     /locus_tag="ACHKNP_RS06875"
                     /old_locus_tag="pgaptmp_001375"
     CDS             10057..10608
                     /gene="maa"
                     /locus_tag="ACHKNP_RS06875"
                     /old_locus_tag="pgaptmp_001375"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HGU4703572.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPVRIIKKL"
     gene            10724..11194
                     /gene="ylaC"
                     /locus_tag="ACHKNP_RS06880"
                     /old_locus_tag="pgaptmp_001376"
     CDS             10724..11194
                     /gene="ylaC"
                     /locus_tag="ACHKNP_RS06880"
                     /old_locus_tag="pgaptmp_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HGU4703573.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            11358..12908
                     /gene="pdeB"
                     /locus_tag="ACHKNP_RS06885"
                     /old_locus_tag="pgaptmp_001377"
     CDS             11358..12908
                     /gene="pdeB"
                     /locus_tag="ACHKNP_RS06885"
                     /old_locus_tag="pgaptmp_001377"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HGU4703574.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGQLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(12950..13303)
                     /gene="ybaA"
                     /locus_tag="ACHKNP_RS06890"
                     /old_locus_tag="pgaptmp_001378"
     CDS             complement(12950..13303)
                     /gene="ybaA"
                     /locus_tag="ACHKNP_RS06890"
                     /old_locus_tag="pgaptmp_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HGU4703575.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13423..13519)
                     /gene="ffs"
                     /locus_tag="ACHKNP_RS06895"
                     /old_locus_tag="pgaptmp_001379"
     ncRNA           complement(13423..13519)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKNP_RS06895"
                     /old_locus_tag="pgaptmp_001379"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            13681..13992
                     /locus_tag="ACHKNP_RS06900"
                     /old_locus_tag="pgaptmp_001380"
     CDS             13681..13992
                     /locus_tag="ACHKNP_RS06900"
                     /old_locus_tag="pgaptmp_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HGU4703576.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(14023..14595)
                     /gene="ybaY"
                     /locus_tag="ACHKNP_RS06905"
                     /old_locus_tag="pgaptmp_001381"
     CDS             complement(14023..14595)
                     /gene="ybaY"
                     /locus_tag="ACHKNP_RS06905"
                     /old_locus_tag="pgaptmp_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HGU4703577.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            14813..15673
                     /gene="tesB"
                     /locus_tag="ACHKNP_RS06910"
                     /old_locus_tag="pgaptmp_001382"
     CDS             14813..15673
                     /gene="tesB"
                     /locus_tag="ACHKNP_RS06910"
                     /old_locus_tag="pgaptmp_001382"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HGU4703578.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(15722..17008)
                     /gene="amtB"
                     /locus_tag="ACHKNP_RS06915"
                     /old_locus_tag="pgaptmp_001383"
     CDS             complement(15722..17008)
                     /gene="amtB"
                     /locus_tag="ACHKNP_RS06915"
                     /old_locus_tag="pgaptmp_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HGU4703579.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACFGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(17038..17376)
                     /gene="glnK"
                     /locus_tag="ACHKNP_RS06920"
                     /old_locus_tag="pgaptmp_001384"
     CDS             complement(17038..17376)
                     /gene="glnK"
                     /locus_tag="ACHKNP_RS06920"
                     /old_locus_tag="pgaptmp_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HGU4703580.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(17557..19338)
                     /gene="mdlB"
                     /locus_tag="ACHKNP_RS06925"
                     /old_locus_tag="pgaptmp_001385"
     CDS             complement(17557..19338)
                     /gene="mdlB"
                     /locus_tag="ACHKNP_RS06925"
                     /old_locus_tag="pgaptmp_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU4703581.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(19331..21103)
                     /gene="mdlA"
                     /locus_tag="ACHKNP_RS06930"
                     /old_locus_tag="pgaptmp_001386"
     CDS             complement(19331..21103)
                     /gene="mdlA"
                     /locus_tag="ACHKNP_RS06930"
                     /old_locus_tag="pgaptmp_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU4703582.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHRALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(21133..21591)
                     /gene="decR"
                     /locus_tag="ACHKNP_RS06935"
                     /old_locus_tag="pgaptmp_001387"
     CDS             complement(21133..21591)
                     /gene="decR"
                     /locus_tag="ACHKNP_RS06935"
                     /old_locus_tag="pgaptmp_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HGU4703583.1"
                     /translation="MLDKIDHKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(21744..22562)
                     /gene="cof"
                     /locus_tag="ACHKNP_RS06940"
                     /old_locus_tag="pgaptmp_001388"
     CDS             complement(21744..22562)
                     /gene="cof"
                     /locus_tag="ACHKNP_RS06940"
                     /old_locus_tag="pgaptmp_001388"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HGU4703584.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            22662..24362
                     /gene="ybaE"
                     /locus_tag="ACHKNP_RS06945"
                     /old_locus_tag="pgaptmp_001389"
     CDS             22662..24362
                     /gene="ybaE"
                     /locus_tag="ACHKNP_RS06945"
                     /old_locus_tag="pgaptmp_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HGU4703585.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKHIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            24427..25122
                     /gene="queC"
                     /locus_tag="ACHKNP_RS06950"
                     /old_locus_tag="pgaptmp_001390"
     CDS             24427..25122
                     /gene="queC"
                     /locus_tag="ACHKNP_RS06950"
                     /old_locus_tag="pgaptmp_001390"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HGU4703586.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(25174..25572)
                     /gene="fadM"
                     /locus_tag="ACHKNP_RS06955"
                     /old_locus_tag="pgaptmp_001391"
     CDS             complement(25174..25572)
                     /gene="fadM"
                     /locus_tag="ACHKNP_RS06955"
                     /old_locus_tag="pgaptmp_001391"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HGU4703587.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(25666..26037)
                     /gene="ybaV"
                     /locus_tag="ACHKNP_RS06960"
                     /old_locus_tag="pgaptmp_001392"
     CDS             complement(25666..26037)
                     /gene="ybaV"
                     /locus_tag="ACHKNP_RS06960"
                     /old_locus_tag="pgaptmp_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGU4703588.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(26188..28059)
                     /gene="ppiD"
                     /locus_tag="ACHKNP_RS06965"
                     /old_locus_tag="pgaptmp_001393"
     CDS             complement(26188..28059)
                     /gene="ppiD"
                     /locus_tag="ACHKNP_RS06965"
                     /old_locus_tag="pgaptmp_001393"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU4703589.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKVVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(28251..28541)
                     /gene="hupB"
                     /locus_tag="ACHKNP_RS06970"
                     /old_locus_tag="pgaptmp_001394"
     CDS             complement(28251..28541)
                     /gene="hupB"
                     /locus_tag="ACHKNP_RS06970"
                     /old_locus_tag="pgaptmp_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HGU4703590.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(28732..31086)
                     /gene="lon"
                     /locus_tag="ACHKNP_RS06975"
                     /old_locus_tag="pgaptmp_001395"
     CDS             complement(28732..31086)
                     /gene="lon"
                     /locus_tag="ACHKNP_RS06975"
                     /old_locus_tag="pgaptmp_001395"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HGU4703591.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(31274..32548)
                     /gene="clpX"
                     /locus_tag="ACHKNP_RS06980"
                     /old_locus_tag="pgaptmp_001396"
     CDS             complement(31274..32548)
                     /gene="clpX"
                     /locus_tag="ACHKNP_RS06980"
                     /old_locus_tag="pgaptmp_001396"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HGU4703592.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(32674..33297)
                     /gene="clpP"
                     /locus_tag="ACHKNP_RS06985"
                     /old_locus_tag="pgaptmp_001397"
     CDS             complement(32674..33297)
                     /gene="clpP"
                     /locus_tag="ACHKNP_RS06985"
                     /old_locus_tag="pgaptmp_001397"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGU4703593.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(33544..34842)
                     /gene="tig"
                     /locus_tag="ACHKNP_RS06990"
                     /old_locus_tag="pgaptmp_001398"
     CDS             complement(33544..34842)
                     /gene="tig"
                     /locus_tag="ACHKNP_RS06990"
                     /old_locus_tag="pgaptmp_001398"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGU4703594.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(35186..35503)
                     /gene="bolA"
                     /locus_tag="ACHKNP_RS06995"
                     /old_locus_tag="pgaptmp_001399"
     CDS             complement(35186..35503)
                     /gene="bolA"
                     /locus_tag="ACHKNP_RS06995"
                     /old_locus_tag="pgaptmp_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HGU4703595.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            35808..36386
                     /gene="yajG"
                     /locus_tag="ACHKNP_RS07000"
                     /old_locus_tag="pgaptmp_001400"
     CDS             35808..36386
                     /gene="yajG"
                     /locus_tag="ACHKNP_RS07000"
                     /old_locus_tag="pgaptmp_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4703596.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            36430..37905
                     /gene="ampG"
                     /locus_tag="ACHKNP_RS07005"
                     /old_locus_tag="pgaptmp_001401"
     CDS             36430..37905
                     /gene="ampG"
                     /locus_tag="ACHKNP_RS07005"
                     /old_locus_tag="pgaptmp_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HGU4703597.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTGVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            38365..39312
                     /gene="cyoA"
                     /locus_tag="ACHKNP_RS07010"
                     /old_locus_tag="pgaptmp_001402"
     CDS             38365..39312
                     /gene="cyoA"
                     /locus_tag="ACHKNP_RS07010"
                     /old_locus_tag="pgaptmp_001402"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HGU4703598.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            39334..41325
                     /gene="cyoB"
                     /locus_tag="ACHKNP_RS07015"
                     /old_locus_tag="pgaptmp_001403"
     CDS             39334..41325
                     /gene="cyoB"
                     /locus_tag="ACHKNP_RS07015"
                     /old_locus_tag="pgaptmp_001403"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HGU4703599.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            41315..41929
                     /gene="cyoC"
                     /locus_tag="ACHKNP_RS07020"
                     /old_locus_tag="pgaptmp_001404"
     CDS             41315..41929
                     /gene="cyoC"
                     /locus_tag="ACHKNP_RS07020"
                     /old_locus_tag="pgaptmp_001404"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HGU4703600.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            41929..42258
                     /gene="cyoD"
                     /locus_tag="ACHKNP_RS07025"
                     /old_locus_tag="pgaptmp_001405"
     CDS             41929..42258
                     /gene="cyoD"
                     /locus_tag="ACHKNP_RS07025"
                     /old_locus_tag="pgaptmp_001405"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HGU4703601.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            42270..43160
                     /gene="cyoE"
                     /locus_tag="ACHKNP_RS07030"
                     /old_locus_tag="pgaptmp_001406"
     CDS             42270..43160
                     /gene="cyoE"
                     /locus_tag="ACHKNP_RS07030"
                     /old_locus_tag="pgaptmp_001406"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HGU4703602.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            43345..43656
                     /locus_tag="ACHKNP_RS07035"
                     /old_locus_tag="pgaptmp_001407"
     CDS             43345..43656
                     /locus_tag="ACHKNP_RS07035"
                     /old_locus_tag="pgaptmp_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HGU4703603.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            43640..44182
                     /locus_tag="ACHKNP_RS07040"
                     /old_locus_tag="pgaptmp_001408"
     CDS             43640..44182
                     /locus_tag="ACHKNP_RS07040"
                     /old_locus_tag="pgaptmp_001408"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4703604.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(44238..45173)
                     /locus_tag="ACHKNP_RS07045"
                     /old_locus_tag="pgaptmp_001409"
     CDS             complement(44238..45173)
                     /locus_tag="ACHKNP_RS07045"
                     /old_locus_tag="pgaptmp_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4703605.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELVAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLARSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
BASE COUNT        10761 a        11901 c        11080 g        11446 t
ORIGIN      
        1 tcacgccgca tccggcatga acaacgcgca ctttgtcagt aatctcgggg caactaaatg
       61 tcgccccctt tggttattac cctaccgctg gcgtcgggtc atcgcctttg tagtcgcgtt
      121 tgacttccgt cacctcatcg cccttctcgt tgtgcagatg cacttcaagc tggttaaagg
      181 caatgttgat gtcgttttca cggcacagct gatcgatagt acggttcagc tcatcgacag
      241 tacgactacg gtcacgcagt tcacgcacat acagacgcag ctcatgatcc aacgtgctgg
      301 caccaaacgc cgtaaagaag acttccggca ttggttcgtg catcaccctt gggtgctcag
      361 tcgccgcctt cagtaacact ttacgcactt tttccagatc ggagccatag gccacgccga
      421 gacggatcac cagacgcgta gtagtgtcag tcaacgacca gttgatcaga cgctcggtaa
      481 caaacgcttt gttcgggatg atcacttctt tgcgatcgaa atcggtaatc gttgtcgcac
      541 gaatacggat cttacttacc gtccccgaga agctaccaat ggttaccgta tcgccaatac
      601 gcaccggacg ttcgaacaga atgatcaaac cggagacgaa gttaccgaaa atttcttgta
      661 aaccaaaacc aagacctacg gataatgctg cggccagcca ctggagttta tcccaagaga
      721 cgcccagcga tccgaacacc gtcatcgcac caacggcaat aatgatgtag ttaaggatgg
      781 tagtaatggc atacgacgcg ccctgacgca tattcagtcg cgagagcacc agcacttcca
      841 gtaaaccagg caggttgcga atcaacgccc aggccaccat tgaggcgata atcgcaaaca
      901 acagactgcc catggtgacg tttttcacca ccgcagcgcc agcttcagtg ccgttgtaat
      961 gccagagcgt gatgctgtcg agatagctga acacggtgat caaatcggac caaattgccc
     1021 agaacatgac accgaacagc gcaaacatca gcaacatggt aatacgcagc gtctgctggt
     1081 taacctgttc cagtgcaatg gtgggttctt ccggcggttc ggcaccttct gcgccctctt
     1141 tcaccagatt ctgccgacgc gccaacgcac gacgccaggc gatacgccgc gccgctacgc
     1201 ttaagccgcg caataccgtc tggtagagca agttccagat gatcaccaga taaacggttt
     1261 caatccaacg cccggagagg cgcagcgtgg tgtagaaata acccgtggca gtcagcacca
     1321 tcaatgcaat cgggataatc gacagcacgg taatggtgac cagtcgcatg gtgtgcgact
     1381 ctttatcacg ccagctttcg cggcacattg gccataccag gaaggcaatc agcagcaggt
     1441 tgaagaaaat catcgcctgc cccagcacat catccatcag atgcagcggg gaaagttctg
     1501 ccaccacaga ccagaaatgg ataggcagca acgcgagact gatgcggaca atttgccgac
     1561 gccagtggct ggtctgctgc tccggcatgc cgaagtgacg cacggcaacg ccatttttct
     1621 ccagtacctt ccagcacagg ccaaacacca gccagaaaat cgccagtttt ttactgaacg
     1681 accatagcag ttcgctgatg ttgagctgca tggtcaacag aatcaggcca accgcgagaa
     1741 taatcaggca caccggcagc gcgcggatca ggtcgataag gatcgctttt ggtgtgttga
     1801 gctggctatc gttacgcagg gaacccacag cggaagccag tttctgttga tacgctttca
     1861 gccagcccag acgccagtgg atcagcccgg caatcaacag cagcggcaaa ccggcgagga
     1921 aagcgataaa aacggcaggc caggcttttt cccagttcac cgtgattttc atcgacttaa
     1981 attcatcttt caggctttgc gggaacgctt tgatccagtc ccagtccatt ggacggttac
     2041 tgttcaccca aaagatttgc tgagtcagga tggatttcag gtttttcgat acgctcatta
     2101 actgctgctg gttgatttgc aggttaatgg ccatcatcag ctggttaccc aactgtttgt
     2161 tgagttgatc cagcaattcg cgacgcatat caaccacttg caataacgca tcgtgaactt
     2221 cgctgttgac ttcgttggtg tgaccttctt ccagtttgtt gacgaacgca tcgctctgga
     2281 agagtgcatc acgctgctgg ttaacttcaa actgttcgag acgcaaatcc gcgatgcggt
     2341 tggtcatgtt ttccagttca tccgccgagg gcagcgtttg ttgttgctgg taaaggatac
     2401 gagacaacag caggctgccc ttcaggacgg caatctgctc tttaatattg cgttccgatt
     2461 gcagcgcccg ctccagccag tttttgactt taatgttttg ctgcatcaac tgattaccgt
     2521 tttcagtcgc ggtaatcaga cgctgactta actgctggtt aatttccagt tcctgtttca
     2581 ccagcggatt agcctgaata cgcgcggctt catccgggga gacggcttcc tgcgccgttt
     2641 tttcagttaa agtcaggcgc ttgctgttta ccgcttcttg caacagctgc aactggtgct
     2701 ccagacgggc gctgttcgcc gtcacgtaat cacgttgctt ttgcaaggta tcctgcaaga
     2761 cggtgttccc ttccaggctt ttacgctgct ggtcaatctc cgcattcagc aacgcctgct
     2821 gagcctgcat taacactttc tggctgggac gtaaggcagt ctcgccgaca tcagtcccat
     2881 ccagacgact gcgaatttgt tgcagctgct gcgaagcgtt atacatcgca ttttgcacgc
     2941 gttcgggctg cgtctgtaac gaaaccagct ggctgttata agacgccaga tcgttttgtg
     3001 cgttttgcaa atcgtccagc gcctgggcaa cgcgagtttc cagctggcgc aacgaaagcg
     3061 tactcagaat tttgcgcgtt tcttcgtcgt tatcgacatc gctaagtgct gttaacgccg
     3121 cggtcgcctg gcgcattttt tccggcgctt cagcgacttt ttgtcgtagc tgaactgtct
     3181 cttcttttat gcgatcgatt ttatcgaggg tggctaatgt atctgtcaga tcctgctgca
     3241 ccagtttgtc ctgagcagaa agatcttttt gtttatttag tgagtcaagt tgcgcctgca
     3301 ggtccgcttt tgtcggcaga tcaccattcg atgacgcccg cgcaaacgcc gtgttctggc
     3361 ataacaagac aaaaacaaaa gcaataaatg ctgaaaaaac aaaatgccgt gatcgtttgt
     3421 aatactggaa catagtcatg atgaatgaag gtttctgaac ctgaagaacg acctgaaaaa
     3481 gtcaaaccgc aagaatatca cgacgcagtg aaccagaata gcaacgacga aaatgtccag
     3541 gaaaaatcct ggagtcagat tcagggttat tcgttagtgg caggattacg aagcgtgggg
     3601 cacaggagat acatctccag taagatggca acgtaatcgc gggcttcttt tttaagatca
     3661 aaagattgcg gggcaaagag ccagttttcc atcaggccgg aaatatagcc gcgcataata
     3721 attgctgcgc gacgcgtcat taaatccgca ggcaacattt tcgcttcaat acaatgtttt
     3781 aacgtttgtt ctatacggtc ataactttcc agacagagat tacgttgtgc ctgttgcaca
     3841 acagccattt ctccgacaaa ttcgcatttg tggaatataa tctccatcaa taatcgacgc
     3901 cgttcttctg tcaccgtgga ttcaagaaca tgaattaata tctctcttaa tactgagagt
     3961 ggatcgccag ggaattttac ctgatactca agctctagtt caccaatatt ggattctgac
     4021 agttcccaga tctcactgaa caaatccgac ttgtctttaa aatgccagta gattgcaccg
     4081 cgcgtaacgc cagctgcttt tgcaatctca cccagcgagg tggatgatac cccctgctgt
     4141 gagaaaagac gtagagccac atcgaggatg tgttggcgcg tttcttgcgc ttcttgtttg
     4201 gtttttcgtg ccatatgttc gtgaatttac aggcgttaga tttacataca tttgtgaatg
     4261 tatgtaccat agcacgacga taatataaac gcagcaatgg gtttattaac ttttgaccat
     4321 tgaccaattt gaaatcggac actcgaggtt tacatatgaa caaaaacaga gggtttacgc
     4381 ctctggcggt cgttctgatg ctctcaggca gcttagccct aacaggatgt gacgacaaac
     4441 aggcccaaca aggtggccag cagatgcccg ccgttggcgt agtaacagtc aaaactgaac
     4501 ctctgcagat cacaaccgag cttccgggtc gcaccagtgc ctaccggatc gcagaagttc
     4561 gtcctcaagt tagcgggatt atcctgaagc gtaatttcaa agaaggtagc gacatcgaag
     4621 ctggtgtctc tctctatcag attgatcctg cgacctatca ggcgacatac gacagtgcga
     4681 aaggtgatct ggcgaaagcc caggctgcag ccaatatcgc gcaattgacg gtgaatcgtt
     4741 atcagaaact gctcggtact cagtacatca gtaagcaaga gtacgatcag gctctggctg
     4801 atgcgcaaca ggcgaatgct gcggtaactg cggcgaaagc tgccgttgaa actgcgcgga
     4861 tcaatctggc ttacaccaaa gtcacctctc cgattagcgg tcgcattggt aagtcgaacg
     4921 tgacggaagg cgcattggta cagaacggtc aggcgactgc gctggcaacc gtgcagcaac
     4981 ttgatccgat ctacgttgat gtgacccagt ccagcaacga cttcctgcgc ctgaaacagg
     5041 aactggcgaa tggcacgctg aaacaagaga atggcaaagc caaagtgtcg ctgatcacca
     5101 gtgacggcat taagttcccg caggacggta cgctggaatt ctctgacgtt accgttgatc
     5161 agaccactgg gtctatcacc ctacgcgcta tcttcccgaa cccggatcac actctgctgc
     5221 cgggtatgtt cgtgcgcgca cgtctggaag aagggcttaa tccaaacgct attttagtcc
     5281 cgcaacaggg cgtaacccgt acgccgcgtg gtgatgccac cgtactggta gttggcgcgg
     5341 atgacaaagt ggaaacccgt ccgatcgttg caagccaggc tattggcgat aagtggctgg
     5401 tgacagaagg tctgaaagca ggcgatcgcg tagtaataag tgggctgcag aaagtgcgtc
     5461 ctggtgtcca ggtaaaagca caagaagtta ccgctgataa taaccagcaa gccgcaagcg
     5521 gtgctcagcc tgaacagtcc aagtcttaac ttaaacagga gccgttaaga catgcctaat
     5581 ttctttatcg atcgcccgat ttttgcgtgg gtgatcgcca ttatcatcat gttggcaggg
     5641 gggctggcga tcctcaaact gccggtggcg caatatccta cgattgcacc gccggcagta
     5701 acgatctccg cctcctaccc cggcgctgat gcgaaaacag tgcaggacac ggtgacacag
     5761 gttatcgaac agaatatgaa cggtatcgat aacctgatgt acatgtcctc taacagtgac
     5821 tccacgggta ccgtgcagat caccctgacc tttgagtctg gtactgatgc ggatatcgcg
     5881 caggttcagg tgcagaacaa actgcagctg gcgatgccgt tgctgccgca agaagttcag
     5941 cagcaagggg tgagcgttga gaaatcatcc agcagcttcc tgatggttgt cggcgttatc
     6001 aacaccgatg gcaccatgac gcaggaggat atctctgact acgtagcggc gaatatgaaa
     6061 gatgccatca gccgtacgtc gggcgtgggt gatgttcagt tgttcggttc acagtacgcg
     6121 atgcgtatct ggatgaaccc gaatgagctg aacaaattcc agctaacgcc ggttgatgtc
     6181 attaccgcca tcaaagcgca gaacgcccag gttgcggcgg gtcagctcgg tggtacgccg
     6241 ccggtgaaag gccaacagct taacgcctct attattgctc agacgcgtct gacctctact
     6301 gaagagttcg gcaaaatcct gctgaaagtg aatcaggatg gttcccgcgt actgctgcgt
     6361 gatgtggcga agattgagct gggtggtgag aactacgaca tcatcgcaga gtttaacggc
     6421 caaccggctt ccggtctggg gatcaagctg gcgaccggtg caaacgcgct ggataccgct
     6481 gcggcaatcc gtgctgaact ggcgaagatg gaaccgttct tcccgtcggg tctgaaaatt
     6541 gtttacccgt acgacaccac gccgttcgtg aaaatctcta ttcacgaagt ggttaaaacg
     6601 ctggtcgaag cgatcatcct cgtgttcctg gttatgtatc tgttcctgca gaacttccgc
     6661 gcgacgttga ttccgaccat tgccgtaccg gtggtattgc tcgggacctt tgccgtcctt
     6721 gccgcctttg gcttctcgat aaacacgcta acaatgttcg ggatggtgct cgccatcggc
     6781 ttgttggtgg atgacgctat cgttgtggta gaaaacgtgg agcgtgttat ggcggaagaa
     6841 ggtttgccgc caaaagaagc tacccgtaag tcgatggggc agattcaggg cgctctggtc
     6901 ggtatcgcga tggtactgtc ggcggtattc gtaccgatgg ccttctttgg cggttctact
     6961 ggtgctatct atcgtcagtt ctctattacc attgtttcag caatggcgct gtcggtactg
     7021 gtagcgttga tcctgactcc ggctctttgt gccaccatgc tgaaaccgat tgccaaaggc
     7081 gatcacgggg aaggtaaaaa aggcttcttc ggctggttta accgcatgtt cgagaagagc
     7141 acgcaccact acaccgacag cgtaggcggt attctgcgca gtacggggcg ttacctggtg
     7201 ctgtatctga tcatcgtggt cggcatggcc tatctgttcg tgcgtctgcc aagctccttc
     7261 ttgccagatg aggaccaggg cgtatttatg accatggttc agctgccagc aggtgcaacg
     7321 caggaacgta cgcagaaagt gctcaatgag gtaacgcatt actatctgac caaagaaaag
     7381 aacaacgttg agtcggtgtt cgccgttaac ggcttcggct ttgcgggacg tggtcagaat
     7441 accggtattg cgttcgtttc cttgaaggac tgggccgatc gtccgggcga agaaaacaaa
     7501 gttgaagcga ttaccatgcg tgcaacacgc gctttctcgc aaatcaaaga tgcgatggtt
     7561 ttcgccttta acctgcccgc aatcgtggaa ctgggtactg caaccggctt tgactttgag
     7621 ctgattgacc aggctggcct tggtcacgaa aaactgactc aggcgcgtaa ccagttgctt
     7681 gcagaagcag cgaagcaccc tgatatgttg accagcgtac gtccaaacgg tctggaagat
     7741 accccgcagt ttaagattga tatcgaccgg gaaaaagcgc aggcgctggg tgtttctatc
     7801 aatgacatta acaccactct gggcgctgca tggggcggta gctatgtgaa cgactttatc
     7861 gaccgcggtc gtgtgaagaa agtttacgtc atgtcagaag cgaaataccg tatgctgccg
     7921 gatgatatcg gcgactggta tgttcgtgct gctgatggtc agatggtgcc gttctcggca
     7981 ttctcctctt ctcgttggga gtacggttcg ccgcgtctgg aacgttacaa tggcctgcca
     8041 tccatggaaa tcttaggcca ggcggcaccg ggtaaaagta ccggtgaagc aatggagctg
     8101 atggaacaac tggcgagcaa actgcctacc ggtgttggct atgactggac ggggatgtcc
     8161 tatcaggaac gcctctccgg caaccaggca ccttcactgt acgcgatttc gttgattgtc
     8221 gtattcctgt gtctggcggc gctgtacgag agctggtcga ttccgttctc cgttatgctg
     8281 gtcgttccgc tgggggttat cggtgcgttg ctggctgcca ccttccgtgg cctgaccaat
     8341 gacgtttact tccaggtagg cctgctcaca accattgggt tgtcggcgaa gaacgcgata
     8401 cttatcgtcg aattcgccaa agacttgatg gataaagaag gtaaaggtct gattgaagcg
     8461 acgcttgatg cagtgcggat gcgtttacgt ccgatcctga tgacctcgct ggcgtttatc
     8521 ctcggcgtta tgccgctggt tatcagtact ggtgctggtt ccggcgcgca gaacgcagta
     8581 ggtaccggtg taatgggcgg gatggtgacc gcaacggtac tggcaatctt cttcgttccg
     8641 gtattctttg tggtggttcg ccgccgcttt agccgcaaga atgaagatat cgagcacagc
     8701 catactgtcg atcatcattg atacaacgtg taatcactaa ggccgcgcaa gcggcctttt
     8761 ttatgcataa cctacgaaca ttaaggagta attgaaccac caactcagga tctcatacga
     8821 aaaccagtat taaccacgga taaaattcat aaaaaatact gattgttagt taatttatat
     8881 taagtagcgc taatagattt aataatccat aatcatttag aggctattct taattatttg
     8941 cggtaattct ttattcattc ctcggttatt acgtcatatt cagagcaatc ctggtattag
     9001 tgtcaccaat ttcatctggc gataatcctg aaatgttatg aacagttcga gcaaactgct
     9061 tttacctgct gcgggttagt gctaatatga aaaagtgagt cctgtcccga ttccttccta
     9121 attgtaattt ttcgtaataa tgcgatgaaa acctgcaaag agtggcttat agttaagcta
     9181 acaaacgaga gggcaagtcc aggtcagtaa gttttttcca tcccgaaagg tgtccgttag
     9241 ttcaaccact aagaagggga cgcgttatgg atgaatactc acccaaaaga catgatatcg
     9301 cacagcttaa gtttctctgt gaaaccctgt atcatgactg tcttgcaaac cttgaagaaa
     9361 gcaatcatgg ctgggttaac gacccaacct cggcgatcaa cctccagttg aatgaactga
     9421 ttgagcatat tgcgaccttc gcacttaatt acaaaattaa gtataatgaa gacaataagc
     9481 tcattgagca gatcgacgaa tatctggatg acacctttat gttgttcagc agttatggta
     9541 ttaatatgca ggatcttcag aaatggcgga agtcaggtaa tcgactattc cgttgttttg
     9601 tcaatgcgac gaaagagaat cctgcgagtt tatcttgtta gaattattac aaccatgggt
     9661 agaagtatgt ccgaaaaacc tttaacgaaa accgattatt taatgcgttt acgtcgttgc
     9721 cagacaattg acacgctgga gcgtgttatc gagaaaaata aatacgaatt atcagataat
     9781 gaactggcgg tattttactc agccgcagat caccgcctcg ccgaattgac catgaataaa
     9841 ctatacgaca agatcccttc ctcagtatgg aaatttattc gctaataaat aattcgcttt
     9901 cggagctata accggctgtt tattaagaat tttatacttt ttcaccatga agacataccc
     9961 tatgtgatct ttatcacaca gatgtaatag gaacgttctc ttcactgact tttcgtctta
    10021 ctgtgttgcc gcattttcag caaccggagg tcagtaatga gcacagaaaa agaaaagatg
    10081 attgctggtg agttgtatcg ctcggcagat gagacgttat ctcgcgatcg cctgcgcgct
    10141 cgtcagctta ttcaccgata caatcattcc ctggcggaag agcacacatt acgccagcaa
    10201 attctcgctg atctattcgg tcaggtgaca gaggcttata ttgagccaac gtttcgctgt
    10261 gactatggct ataacatttt tctcggtaat aattttttcg ccaacttcga ttgcgtgatg
    10321 cttgatgtct gccctattcg catcggtgat aactgtatgt tggcaccagg cgttcatatc
    10381 tacacggcaa cacatcccat cgaccctgta gcacgtaata gcggtgctga actggggaaa
    10441 cccgtcacca tcggtaataa cgtctggatt ggcggacgcg cggtcattaa ccctggtgtg
    10501 accattggtg ataacgtcgt ggtagcctca ggtgcagttg tcacaaaaga tgtcccggac
    10561 aacgttgtcg tgggcggtaa tccagtcaga ataattaaaa aattgtaatc ggtttttcgc
    10621 aactgttatg caaaattgtg gtagatctgt tacttcccct ctactattcc cacgttaaaa
    10681 tagggtgttc cctggaaagt tgcagatacc acgaaggcaa acgatgaccg aaatacaacg
    10741 cctgctgacc gaaacgattg agtctctgaa tacccgcgaa aaacgcgaca acaaaccccg
    10801 ctttagtatc agttttatcc gtaaacatcc ggggctgttt atcggtatgt acgttgcttt
    10861 ttttgccacc ctggcggtga tgttgcagtc cgaaacgctg tcaggctctg tctggctact
    10921 ggttgtatta tttatcctgc ttaatggttt cttctttttc gatgtctacc cacgctaccg
    10981 ctatgaagat atcgacgtgc tggatttccg cgtttgctat aacggcgaat ggtacaacac
    11041 gcgctttgta cctgccgcgc tggttgaagc catcttgaac tctccgcgtg tcgcggatgt
    11101 tcataaggaa caactgcaaa aaatgatcgt ccgtaaaggt gaactgtctt tttacgatat
    11161 ttttaccctc gctcgcgccg aatcaacatc ttaagttagg gttacatacc aggcgtaaag
    11221 ctctgcgcct ggtcaaatga caatgatcgt ttccacccat cacttcatga aataccagct
    11281 ctacctcctt atctcctgcc agcctttttc cacaatcaga tatactttcc ctacactgtg
    11341 ttaataagga tatgctggtg agaacacgac atctggtcgg ccttatttcg ggagtactga
    11401 ttctttcagt attgctgcct gtcggcttaa gcatctggct ggcccatcag caggtagaaa
    11461 catcgtttat tgaagagctg gatacctatt cctcccgcgt cgctattcgg gccaataagg
    11521 tggcgacaca agggaaagat gcgctgcaag agctggaaag atggcaaggc gctgcctgta
    11581 gcgaagccca tctcatggaa atgcgtcggg tatcttacag ttatcgctat attcaggaag
    11641 tggtttatat cgataacaac gttccccagt gttcgtctct ggagcatgaa agtccgcccg
    11701 ataccttccc cgagccaggt aaaatttcga aagatggtta tcgtgtctgg ttaacatcgc
    11761 ataacgattt aggcattatc cgttacatgg tcgccatggg aacggcacat tatgtcgtca
    11821 tgatcgaccc cgcttccttt attgatgtca ttccctatag ctcatggcaa attgatgccg
    11881 ccattattgg caatgcccat aacgttgtca taaccagcag cgatgaaatt gctcagggaa
    11941 ttattaccag gctacaaaaa acacccggtg agcatatcga aaataatgga atcatttacg
    12001 atatcctgcc cttcccggag atgaatattt cgatcatcac ctgggcttca acgaaaatgt
    12061 tgcagaaagg ctggcatcgg caagtcttta tttggttacc gctcgggttg gtgattggcc
    12121 agctggcagc gatgtttgtg ctgcgtattt tgcgccgtat ccagtcaccg catcatcggc
    12181 tgcaggatgc tatcgaaaat cgtgatattt gcgtgcacta tcagccgatt gtctccttag
    12241 ccaatggcaa aattgtcggt gctgaggcac tggcgcgctg gccgcagaca gacggtagtt
    12301 ggttgtcacc agatagtttt attccgctgg cacagcaaac gggcctttct gagccattga
    12361 cgctactgat tataagaagc gtctttgaag atatgggcga ctggctgcgt cagcatccac
    12421 agcagcatat ttcgatcaat cttgaatcca ccgtgctcac ctcggaaaaa atcccgcaat
    12481 tgctgcgtga aatgatcaat cactatcagg ttaatcccag acagatcgcg cttgaactca
    12541 ctgaacgcga gtttgccgat ccgaaaacca gcgccccgat aatttctcgc taccgggagg
    12601 cgggccatga aatttatctt gatgattttg gtacgggata ttcaagttta agttatttac
    12661 aggatctgga tgtcgacatt ctgaagatcg ataaatcttt cgttgatgcg ctggaatata
    12721 aaaatgtcac gccgcatatc atcgaaatgg caaaaacact gaaactgaaa atggtagcgg
    12781 agggaatcga aaccagtaaa caagaagagt ggctacgcca gcatggcgtg cactacggtc
    12841 agggctggct ctacagcaag gcattaccga aagaagattt cttacgctgg gccgagcaac
    12901 atttgtgaat ttttgggctg acggcacgcc gcacagcccg tcacgctacc tattcgtcga
    12961 tgattgactc gaatccgcca tagatcattc gcttgccgtc aaacggcatg gactcgccga
    13021 actctttcat ccgtgggtcc gacatcatct tttgattagc agcgtcgcgg acctcttttg
    13081 aagggtattc aatccagcta aagacaacct cttcattctc ttccgctttc accgccatac
    13141 gaaaatcggt cactttgcca tccggtacat cgctggccca gcattcgaca atacgaagcg
    13201 cgccaaactc tttaaacaat ggcgcagcct tagcggccat ttcccgatag gcatcctttt
    13261 tatcggcagg aacggcaacc acaaaaccat caacatactt catcagacat acctccgaag
    13321 cgtactgcgc agccagacaa ttcttgcgac gtcagttgtg gttaaagttt agccatgttg
    13381 gttttttttg acgaaacggt gggaggcaga aatgggtggg ggccctgcca gctacatccc
    13441 ggcacacgcg tcatctgcct tggctgcttc cttccggacc tgacctggta aacagagtag
    13501 cgttgcggga gaaccaacag gcccccattg agagcgttga gaaccaacgc gcaggcgcat
    13561 tatcactgct gccccggcta attgcaaccc atcgcgtgcc agatgctggt ttcttgcgca
    13621 atgcgacttc actcgggcgt ttttccagat tatgctgaaa aattacctca ggaagaaaag
    13681 atggaaaaag aagattcatt tccccaacgc gtctggcaaa tcgtcgccgc tattcccgaa
    13741 ggctatgtca ccacttacgg tgatgtggcg aaactggcgg gatcgccccg cgccgcgcgc
    13801 caggtgggcg gtgtgttaaa gcgtctccct gaaggcagca ccttaccctg gcaccgggtg
    13861 gttaatcgcc acggcacaat ttcgctaacc ggaccggatt tacagcgtca gcgacaggca
    13921 ttactggcag aaggtgtgat ggtatcggga agcgggcaaa tcgacttgca gcgttatcgc
    13981 tggaactact aaccctctcc gggcggagag ggttaacgtc gcttaatatt gcgtaggagc
    14041 tggaactgcc gaagacggat tcacctgagt tggtgaagtc gaaggtacgg tagtcgttgc
    14101 accaccgctg gcctgaaccg gcacggcggt ttgctgcacc ggcaccaatg tcaggtcggc
    14161 tttagttccg cccttgttga tcaccggctg aacggtatcg gtaataaata ccagtttgtc
    14221 attcacggta atcgccgcac tcaacagaat acgcgcgttc ggctgaacat ctgccggatt
    14281 aaatggcaga acaaagctga atggtgactg tttaccttcg gtacgcaccg ctttctgcgc
    14341 cagcactttt gacggtgcat cggctaacga cgcgtcagaa agtgtcacgg tcagcacagc
    14401 atcaggcggc agtgcgactt tctgccggat ccagacggta ccggagacat tcggttgctg
    14461 gatagcaggt tgttgtgttg ctgaaataga tgtatttgca gccggggctg gcgtctgaat
    14521 atccgcgctt ttatctgcgc aagccgccaa cgcaatcgca accgctaaac cactggccat
    14581 gtgcacgagt ttcattcatt tctccttatt atcaatgcac cagcgggcta actttcctcg
    14641 ccggaagagt ggttaacaaa atagtaacgt caacaagtgt ggcacacatc acgcatttct
    14701 gcctgtaatt agcccgtaat tcagaccatt gcacccatcg gaccagtagc aaatttgcgt
    14761 tatactcaac tcactttggc ttgctgcggc agctttgtta ctggagagtt atatgagtca
    14821 ggcgctaaaa aatttactga cattgttaaa tctggaaaaa attgaggaag gactctttcg
    14881 cggccagagt gaagatttag gtttacgcca ggtgtttggc ggccaggtcg tgggtcaggc
    14941 cttgtatgct gcaaaagaga ccgtccctga agagcggctg gtacattcgt ttcacagcta
    15001 ctttcttcgc cctggcgata gtaagaagcc gattatttat gatgtcgaaa cgctgcgtga
    15061 cggtaacagc ttcagcgccc gccgggttgc tgctattcaa aacggcaaac cgatttttta
    15121 tatgacggcc tctttccagg caccagaagc gggtttcgaa catcaaaaaa caatgccgtc
    15181 cgcgccagcg cctgatggcc tgccttcgga aacgcaaatc gcccaatcgc tggcgcacct
    15241 gctgccgcca gtgcttaaag ataaattcat ctgcgatcgt ccgctggaag tccgtccggt
    15301 ggagtttcat aacccactga aaggtcacgt cgcagaacca catcgtcagg tgtggatccg
    15361 cgcaaatggt agcgtgccgg atgacctgcg cgttcatcag tatctgctcg gttacgcttc
    15421 tgatcttaac ttcctgccgg tagctctaca gccgcacggc atcggttttc tcgaaccggg
    15481 gattcagatt gccaccattg accattccat gtggttccat cgcccgttta atttgaatga
    15541 atggctgctg tatagcgtgg agagcacctc ggcgtccagc gcacgcggct ttgtgcgcgg
    15601 tgagttttat acccaggacg gcgtgctggt tgcctcgacc gttcaggaag gggtgatgcg
    15661 taatcacaat taaaaaaaat agccggaggt aaaaccgtcc ggctattttt tgcagtgctt
    15721 gttacgcgtt ataggcattc tcgccgtgac tgttgacatc cagcccttct cgctcctgct
    15781 cttccggtac acgcagacca accgtcagat ccgccagttt gtagccaata aatgccacca
    15841 caccggacca gacgatcgta atggcgatgc tttccagctg taccagcaac tgatggccca
    15901 tcgtcacacc ttcagcgaag cccacgccac ccagcgaact ggcggcaaaa atcccagtca
    15961 tgatacagcc gacaatgcca caaacgccgt gcacaccgaa gacatcgcag ggatcatcca
    16021 cccgcagcaa gcgtttgagc atagtaacgc cccacaagcc cgccagacca gctaccacgc
    16081 cgataatcaa cgcgccgcca accccaatgt agccacacgc tggcgtcacg ccgaccagac
    16141 cggcaatcgc gccaaaacac gctcccagca gagaaggctt accacgcagc gcccattcac
    16201 cgaagatcca gccaagaatt gccgccgccg ttgcgaccac agtattcaca aatgccagtg
    16261 ccgcgatttc attcgccgtg cccgctgacc cggcgttaaa gccaaaccaa ccaatataga
    16321 gaatggcagt accggtgaag accattggca ggttgtgcgg tttaaacgcc tctttgccga
    16381 agcccacgcg tttgcctatc agatacgcgc ccaccagacc ggcgattgcg gcgttaatat
    16441 gcaccaccgt gccacccgcg aaatccagcg caccgtgaga agccagcaaa ccaccgcccc
    16501 acaccatatg cgcaatcgga atgtaagaga gcgtcagcca taccaccacg aaaatcaaca
    16561 cagctgagaa gcggattcgt tccgccagcg ccccaacgat caagccgacg gtaatgcagg
    16621 caaacgagcc ctgaaacgcc acgtggatat actgataaat gctgcccatc accgccgtca
    16681 gttcgatgtt ttttagcatc aaccagttaa tgttgccgaa gaagttgttg ccctcaccaa
    16741 acgccagcga gtaaccgtaa accacccaga gaatacagac cagtgcaaac gtcaccgtca
    16801 cctgcgtcag catcgacagc acgtttttgc cgcgaatcaa cccaccgtaa aacagggcaa
    16861 tccccggaat agtcataaac agcaccagcg cagtacaaat catcataaac gcattgtcgg
    16921 ctttatcggc caccgcaggt gcagccatta ccagtcccgg cagcatcgcc agtgaagcaa
    16981 gcccagtttt tatcgtcgct atcttcattt ttcgttcctg ttgctgtgtg ccagagatta
    17041 cagcgccgct tcgtcggctt cgccggtacg aatacgaatg acgcgttgca attcagcgac
    17101 gaagattttg ccgtcgccaa tttttccggt gtaagccgcc ttactgacga tatcgatcac
    17161 ttcatcgagt tggtcatcag caatcgccac atcaattttt acttttggca ggaagttgac
    17221 gctgtattcc gccccccggt acagctcggc atgccctttc tgacgcccga aacctttcac
    17281 ttcggtgacg gtcaggccct gaataccaat ggaagataac gcttcacgaa cgtcttccag
    17341 cttgaatggt ttgattatca cggtcaccag cttcatagat cccctccggt cagaattcgg
    17401 taatggttct gctacacgaa gaaggtattg caagcggtgt gccagaaatg aaaaacgaga
    17461 aagagagcag gaagttaaga agattgaaaa tgaaaacgca ctatgacagt gcacagtgcg
    17521 ttacattttt gcaccaacga tgaaatattg cgctattcag gcgctcaatg attcttcttc
    17581 acgcacgctg gctgccagct cttcgcccgc aagttgcagt tgatacatct gccagtagcg
    17641 tccctgggcc gccagcagtt gctggtgagt gccctgctcc acggcttgcc cacgatgaag
    17701 caccagaatg gtgtcggcat caacaatggt cgataagcgg tgagcaatca ccaccagcgt
    17761 ggtatgttca cgcacagccg ccagagcatg ttgaatcgcc tgttcagtac cggagtcaat
    17821 gctggcggtt gcctcatcaa ggatcaggat ttgcggcgtc tccaccaaca cgcgtgccag
    17881 tgccagcagt tgcttttgcc cgacggagag attattcccc tgctcgccca gcggcgtata
    17941 aataccatca ctcatgctgc gcgccagctc cgccagttgc acggtttcca gcgcctgcca
    18001 gacgcgttct tcggagatat cacgccccag cgtcacgttg gcgaggaagg tatccgccag
    18061 caccaccgga tcttgctgca ccattgccac gccctggcgc agcgcgctgt gacttagcga
    18121 acttaatgga cgaccatcaa ggcgaatctc accttccgtt agcgggtaat agcccatcaa
    18181 taaactggcg agggtgcttt tgccactgcc ggtatgcccg accagtgcca caaagttacg
    18241 cgaaggcaca gagagattaa tgttctttag caccagattg tcatcgcgat aagcaaatga
    18301 cacgttatcg acttcgatgg tgccactctg taacgggcga tcatcattgc catattgctg
    18361 gcgcggtccg tccatcagtt caaacacgcg ctcaccagca acaacagcct gttgcagcat
    18421 cgcctgttgc gtggtcagtt cgattaatgg ttcgttaagt cgcccaagat agctgataaa
    18481 cgcatacagc acgcccactt caatggtgcc gctagcggag aagccaaaca gcatcaacaa
    18541 gccacaaaga atgagcgatg aaaacagact cagcagcgga cgcagcagaa aaccgtcgag
    18601 gcgcagggtt tgcatcctgg ccatatagtg tgaacgactg gcctccccca tacgttcgcc
    18661 aaatcgcgcc tgctgacgga actgctggat aacgctcatg ccattgatga tttcgttaaa
    18721 gccgtcgttg atatccgcta gataggcgcg cacacgacgg acaatcggcg tgctgtaacg
    18781 ctggtatatc accattacca ccagcaccac cgggaaaatc attatcgcca ccagcgccat
    18841 tcgccagtcg aggctgaaca tcgccaccag catcgcgccc accagtgcgg cgctacgcag
    18901 gacagttgcc actacggtaa cgtagagatc gcggatcact tcagtgtcat tagtgacgcg
    18961 ggaaatcacc tgcccgacgg gttgggtatc aaactcgctt aatggctggc gcaatgcggc
    19021 atccatcacg tcggtacgca actgttgcac tacgcctact gccgcccgat taaacagcag
    19081 cgactgcgcg taatgtagcc cggcggcaaa cagttgtagc ccaacatacg ccgcagccag
    19141 ccctgcaacc attttcaacg gcaggttatt tttcgctacc atattgtcga taaaatagct
    19201 gataagcagc ggcccactga cttctgccgc tgccgcaacc cacatcatca ggaccgcaat
    19261 ccccagcggt ttacgccacg gcgaaccgta cgctaacaga cgcttgagag tcggccacag
    19321 ttggctaaaa ctacgcatcg acggcctcct cgcgaatttc cggagcgtcg tcgagcgccg
    19381 cctccagttg ttgatagcga tacatatcgc gataccagcc gctttgttgc gccagctcat
    19441 catgattgcc acgctgggcg atatgtccgt gctgcatcac aataatttca ctggcttccg
    19501 tcagtgccga aaggcgatgg gcactgatga ttaccgttct accctgtccc cactgacgca
    19561 ggttatgcag gatctggtgc tctgtgcgtc cgtccaccgc cgaaagcgca tcatcaagga
    19621 tgaggatttc cgcgttgact aataacgcac gagcaataga gatacgctgt ttttgcccgc
    19681 cggaaagcat cacgccgcgc tcgcccacct ctgtatcgta accttgcggt aaacgcaaaa
    19741 tatcgtcatg tacgctggct aaccgcgcga catgctcaat ctcttgctgg gtggcattcg
    19801 ggcaacccaa cgcgatgttg ttcgctacgg tgtcggaaaa aaggaacggt gtctggctaa
    19861 ctaccgccag gcggctacgc cagctatcga gttgtaactt cgtcagagga atatcatgaa
    19921 agcgaatatc cccctcgctg acgtcgaaat gacgctgaat gagcgacaac agggtacttt
    19981 tgccggaacc agtcggcccg cagataccca gcatctgacc gggtttcagg gcgaaattga
    20041 cgttttccag cgcacgatgg tcagtctgcg gatacgtgaa ctggtgaata tttacatcaa
    20101 gttcgccacg cccttccggc acgggttcgc taccatcgtt caccaccggc gcttccgcca
    20161 gcatcgcacg aatacggctg tacgcagcac taccacgttc cacaatgtta aacatccatg
    20221 ccagcgccag cattggccaa atcatcagac ctaaatacat cataaaactg gtgagctggc
    20281 ccagcgttaa actgccctgc accaccatcc aactaccgcc gccaatcgcc agcaagttcg
    20341 ccataccaat cgcgatatag attgtcgggt cgaaacgagc atcaatacgc gccacccgca
    20401 tgtttttttt gccggtatct tcggcatccg cggcaaataa cgccgactgg cgatcttcca
    20461 gaccaaaggc tttgatcatg cggatactgg tgaggctttc ctgggtgcgg tcattaagac
    20521 tggaaaacgc cgcctgtgcc agcttaaagc gttcatgcaa ggcatcgccg ttgcgcttaa
    20581 tcatgatcgc catcactggc atcggtaaca gggcaaataa ggtcaactgc cagctaattt
    20641 gcgtagacat cataatcaac acagcgcagc ccattaccag tgaatccacc agcgtcagca
    20701 ccccttctcc ggcggcaaac acgacgcgat cgacgtcatt ggtcgcacga gccatgagat
    20761 cacccgtgcg atgacgcagg taaaactccg ggtgctgccg gcttagctga cggtaataat
    20821 cttcacgcag ttcaacagcc agttggtaag acgcgccaaa cagcaatacg cgccagacat
    20881 aacgcaggag ataaaccaca acggcaatca gcaccatggt ggcgatccac atcaggatca
    20941 gcccggtagt aaagtgttgt tctgtcacgc catcgacaac aataccaacc acttttggcg
    21001 gaaccagttg cagcatcgcg ataatgacaa gcaaggcgac agccccgaga tagcgacgcc
    21061 attcccgacg gaaataccag cttaattgag caaataatcg cacgcgttgt gtcctgacct
    21121 gattctggat atttattcga tgggtaaaga agtggtgtat ttaatctgtt ccatcgcgaa
    21181 gctggaagtg acgtccgaca gccccggcac gctgtttacc agacgcttat aaaactcgtc
    21241 gtagcgtttc atgtcggcaa cctggacgcg catcagataa tcgtattcac cagccatgcg
    21301 ccagaacccc agcacttctg gcatttcggt aaccaccgtg acaaagcggc aataccattc
    21361 gctgctgtga tgttgcgttt ttatcagcac aaaagcggtc aggccgaggc ctattttttc
    21421 cggatccagc agggcgactt tgccgataag gataccgtcg tcctccagcc gtttcagacg
    21481 cttccagcaa ggggtggttg tcagattaac ggcttcagcc agtgcctgca aagagagggt
    21541 gcaatcctgc tgcagtaagg ccagcagctt atggtcaatt ttatctaaca tagcccttcc
    21601 acagagaatt tttttctctt ttaattctat tttaaaggtg aaatcacgac aatttattct
    21661 gtgcggttta aacaccacgg cacaaaatga caaattcaac gcaaaaaaaa ccgggcaatt
    21721 gcccggtgct ggaagggatc tcgttattcg ggggaataag gtagatgtgg atagtccagc
    21781 cagtgcgtca aatagtgaga gacagcctga tttcggcaat gtccaatcac cggtaaatgc
    21841 gggagctccg cgcgcagttg cggcatcgca ttgcccataa taaacccgct accgacgctg
    21901 cctaacattt cgcgatcgtt catcgcatca ccaaaggcca tgcaatcgcg caacgataaa
    21961 cctaaatgtt gggtcagcac cgtcaatgca gcgcctttat tgcagcccac cggcagcact
    22021 tcgaggcaat ccgtggcgga aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc
    22081 tggatctgca agcgtgtaag atcgtcgtga ccgccacaga agcagatctt ggtgactctg
    22141 ccgagtggca tttttttgac atcgattatc tgataacgaa aaccgctata gacaaatgcc
    22201 tgcaacaacg cagggatctc tttcccggta aaccaaccgt cgtcattgaa gatatgcatg
    22261 ctggctcggg tatcccattg ctgatacagc accagctccg cgacatccgc aggtaaatca
    22321 tcacgatgta aaagttcacc ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa
    22381 tatgcatcca gcgatagcgc cccgagaata tgctgcatct ccagcgcatg acgccccgtg
    22441 gcaaaagtga gggtaatgtc gcgttcacgc agtcgcgcca aagtagagag ggttttctca
    22501 cctaaatgat ggtcaggcat caataaagtg ccatccatat caaatgctgc cagacgagcc
    22561 atttctttct ccactctgtg acacggttaa tggtgattga gtatcacctg atatatacgg
    22621 aagtaatagt gaatagttaa ataatattgt tccgggttta tatgcgattg ctcaaccgtc
    22681 ttaaccagta tcaacgtctg tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca
    22741 gcgaactggc cgaacgctgt ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg
    22801 cacaggaggc gggatggctg gagtggcagg cgcagtcagg acgcggaaag cgcggacaat
    22861 tacgctttct ggtcacgccg gaatcgctac gcaatgcgat gatggaacag gcactggaaa
    22921 ccggaaagca gcaagatgtg ctggagctgg cgcaactggc cccaggtgag ctgcgcactc
    22981 tgttacagcc gtttatgggc ggacaatggc aaaacgatac acccacgttg cgtattccct
    23041 actatcgccc gctcgaaccg ctacaaccag gctttttgcc cggccgtgcc gagcagcatc
    23101 tcgccgggca gatattttcc ggcctgaccc gcttcgataa taatactcag cgcccgattg
    23161 gcgatttagc gcatcactgg gaaacctcta ctgacgggtt acgctgggac ttttatcttc
    23221 gttcaaccct acactggcat aacggcgatg cagtaaaagc ctcacactta caccagcgat
    23281 tattgatgct gttacaactg ccagcactgg atcaattatt tattagcgtg aagcatattg
    23341 aagtcaccca tccgcagtgt ctgaccttct ttttacatcg ccccgattac tggcttgcgc
    23401 accggctggc gagctattgc agccatctgg cgcatccgca attcccactg atcggcactg
    23461 gtccttttcg cttaacacaa ttcacagcgg agctggtgcg cctggagagc catgattatt
    23521 accatttgcg tcatccgctg cttaaagcgg ttgagtactg gataactccg ccgcttttcg
    23581 aaaaagattt gggaaacagt tgtcggcatc ccgtgcaaat caccatcggc aaaccggagg
    23641 agctgcaacg ggtcagccag gtcagtagtg gcatcagttt aggtttttgc tatttaacgt
    23701 tgcgcaaaag tccccgactc tccctctggc aggcgcgaaa agtgatctcc attattcatc
    23761 aatccggttt attacaaacg ttagaagtcg gagaaaacct gatcaccgcc agtcatgcat
    23821 tactgccagg ctggactatt ccgtactggc aagtaccgga tgaagtcaaa ctaccgaaaa
    23881 ccttgacgct ggtttatcac ctaccgatag aacttcatac catggcagaa cgcctacagg
    23941 cgacactggc agcggaaggc tgtgaactca caattatttt tcataacgca aaaaactggg
    24001 acgacacgac cctactggca cacgcagacc tcatgatggg cgacagatta attggcgaag
    24061 caccggaata tactctggag caatggctac gttgcgatcc actatggcca catgttttcg
    24121 acgctccagc atatgcacat ctgcaatcga cgctggacgc ggtgcaagta atgcctgatg
    24181 aggaaaaccg atttaatgcc ctgaaagcgg tttttagcca gttaatggca gacgcgacgc
    24241 tgacgccgct gttcaactat cactatcgca ttagtgcccc tcccggcgtg aacggtgtgc
    24301 gactgacacc gcgcggctgg tttgaattta ccgaagcctg gcttcccgcg ccgtcgcaat
    24361 gaatagctgg tccgggttaa tcgcaggcgt taccataacg cttttattgt tcaagcagga
    24421 ttatctatga aacgtgctgt cgttgtgttc agtggaggcc aggattccac cacctgtctg
    24481 gtgcaggcat tacaacaata tgatgaagtc cattgcgtga cgttcgatta cggtcagcga
    24541 catcgcgcag aaatcgacgt ggcacgcgaa ctggcgctga aactgggagc acgcgcgcat
    24601 aaggttcttg atgtaacgct gctcaacgag ctggcggtca gcagcctgac acgtgacagc
    24661 attccggtgc ctgattatga acctgaagcc gatggcatcc cgaatacgtt tgtccctggg
    24721 cgtaatattt tgttcctgac gctggcggca atatatgcgt atcaggtaaa agcagaagcc
    24781 gtaattaccg gcgtctgtga aacggatttc tccggctacc cggattgccg cgatgagttt
    24841 gtgaaagcac taaaccatgc cgtcagtttg ggcatggcga aagatattcg ttttgaaacg
    24901 ccgctgatgt ggattgataa agcggaaacc tgggcgctgg cagattatta cggcaaactg
    24961 gatttagtcc gtaacgaaac gttgacctgc tataacggct ttaaaggcga cggttgcggt
    25021 cattgtgcgg catgtaattt acgcgccaac ggtttgaatc attatctggc cgataaaccg
    25081 acggtgatgg cagcgatgaa gcagaaaacc gggttgaggt aattattccg ggtgtcgccg
    25141 gatgcggctt gagcatccgg caccacaaaa cgtttactta accatctgct ccagcttttc
    25201 gcgcaattcc ccttccagag ctaatgcttt ctgcgtttta agatcaatac aaacaaacgt
    25261 aataagcgca tccgctacca cctgcccttc cggctccagt gtaatgacct ggcttaagat
    25321 gccgctttta ccgtttaatt gctgcaactg actggtaata gttaacaggt cgcttaatac
    25381 cgctgggcga cgatagttaa tattgatatt gaccacgacg aaggcaatgt tatgggccgt
    25441 catccactga aaactgtcgc tattttccaa cccatcccag cgggcttctt cgagaaattc
    25501 aaggtagcgg gcgttgttga cgtgctggta aacgtcgaga tgatatccac gaactttgat
    25561 ttgtgtttgc atagcgcaat aaccttacgt tgttgttata agtacagagg tcataactgg
    25621 tatgacctct tcagtctggc aaaaaattgc cactatgcaa attaattaca gggttaatac
    25681 cgccagatta cgttccacca gcgaattgcc catccccggc acctgcttta gatcctccac
    25741 cgttttaaac ggaccgtact cttcgcgata actgacaatc gcctgcgctt tcttcaggcc
    25801 aacgccattc atcgcgcggg ctagctcttc cgcgctggca ttattaatgc tgacccgggt
    25861 gccttcttcg tcactggctt tcgccggtac tgctgcttta ctttgtgctg caggagcttc
    25921 cgctttggtt tctaccgccg tcggtttcgc cacagaagct gccgccagcg cgctatgaga
    25981 cattccggca caggccaggg acagggtaat gagcagtgct ttaattccgt gtttcatact
    26041 gttttctcct tgtttgttaa cagtggaatc accttaacgg gagtgaaaac agtaaacaaa
    26101 tggcaatgtt cagaaatgga aaaggccgcg aaagcgacct tttcttgtta cagctgcaac
    26161 gcattttttt gcgaggcggc ttcaggatta ttgctgttcc agcgcatcgc caattttgat
    26221 tttcgcctct ttacgcaggt tactcatcag agcttcaaag acgatttgtg cgttgttctg
    26281 ggtgataccc tgcaccatcg cttttttctg atcttccggc attgaacctt gtttcacttc
    26341 atccagcgcc agcagaacca cattaccttg catatcggtc gccataccgt agctcggttt
    26401 gtctttcgct ggcagtggca gtgcaaacgc cgcctggcta atcgggtcac gaccggaacg
    26461 gcttaagatt ttcggctcgc caaatttcag accggcagcc tgcatagctt ccgcaccttt
    26521 gccggctttc aaatcaacca gcagtttctc agcatccact ttcgcctgtt gttcagcttt
    26581 gttgtgctga accagtgcct taacttgttc ctgaacatct gccaacggtt tcaccgcttc
    26641 cggtttgtgc tcgctgatgc gcagcacgaa tgcgcggtcg ccgtctacgg tgatgatgtc
    26701 agagttgatg cccggcgcgc cgttttcacc taccagaccg ccgttaaaga tagcgtcggc
    26761 aaccggcttg aagttcaact cttccggcag gttatctttg ctaaaccaac ccgtctgagt
    26821 ggctttaacg ccggcagctt gctctgcacc ggccagagac tccgtgtcgt tgcttgccgc
    26881 atcgctcact ttctgctgca gcgcatagta cgcatcgagg gctttttcgt gtttcacttt
    26941 cgccgcaatg tcgtcacgta cttcgtctaa cgatttcact ttcgctggct gaatgtcgtc
    27001 cagacgtaca atcaggaaac cgaccgaaga tttgatgaca ccagacagtt gccctttttc
    27061 tttcagacca gcatttttca gttcgtcggg gatagtggca tcttctaacc aacccatatc
    27121 gccgccgtta cgggcagaga taatatcggc agatttttct ttggctaatg cagcaaaatc
    27181 accgccttta ttcagctcat caagtaccac tttcgcttca tcttcagttt tggtctggat
    27241 gatgctgtag cgggtacgct gcggctgggt gaattgatcc tgatgctgat cgtagtagct
    27301 ctggatatcc gcatcgctaa ccggttgctg catcgttgcg gcatccagct tgatgtaact
    27361 cacgcggaat tgttccggcg tcatgaaatt gtttttgttt tgttcgtagt agctggcaat
    27421 ctcctgttcg gtcacaggct gcttcgccgc cagcgcgtta acatcgatag tcgcctcacg
    27481 caccacgcgt tgttgcgcga ccagtgccgc cagctcgtcg gtttcacctt tcagcataaa
    27541 atcggtaccg gcaacaccgt taatcagctg ttgggtagtg agctggttac gcagcgcctg
    27601 ggcgtactga tcggcggtca tccccatctg gttgaggata ccgttatagc ggctgttatc
    27661 aaatttgccg tcaacctgga aggctggggt cgcgaaaatc gcctgtttaa cctgctcatc
    27721 actgataccc agtttcagct cacgagcgta ctgatccaac agcgcctcgt cgatcagacg
    27781 attcagcacc tgttgacgca gggttttcat atagccttcg ttcgctgcca gctcggagta
    27841 ttgatcgccc agctgttgct gcatgcgatt acgctcgctg ttaaaggcgt tctcgaactg
    27901 cccacggctg atttcctggt cattcacttt tgcggcgtag ttattgcctc cgccaatcag
    27961 gtaaccactc acgccggtca atatgaacga cacgataatg ataccgaaaa taatcttgag
    28021 cacgagactg tttgcagccg tgcgtaagct gtccatcatg gtgtaacaac actccgctgt
    28081 agatgactgt gtacctcacg caacgtatcg atgcgcgcaa aggcctattg tgacaagaaa
    28141 cgggggcaat tgtcagccca caagacacag aaagtcaccg aatacaaata aaaaaggcac
    28201 atcagtagat gcgcccttga acttcgtcac atccccactg gggacaacgc ttagtttacc
    28261 gcgtctttca gtgctttacc tgcacggaag ctcggtactt tagcagcagc gatggtgatc
    28321 tctttaccgg tctgcgggtt gcggccagta cgggcagcac gctctttaac ggcaaaagta
    28381 ccaaaaccta ccagtgctac atcatcccct tctttcagag attcagttac ggaagcaata
    28441 atagcatcta acgcacggcc agccgcagct ttagagatat cagcccctgc agcaatcttg
    28501 tcgatcaatt gagatttatt cactcttctc ttcctcttta taatttatat cgcacttgaa
    28561 tccttcaagg tacgaacgcg cagcagttat atcaggccag ccatcccctt acaacacccc
    28621 ttaataaggg caagcccgat ccgccatcta acttagcgag acaaaaaaag gctggcaagc
    28681 ccgaattagc ctgccagccc tgtttttatt agtgcatttt gcgcgaggtc actattttgc
    28741 agtcacaacc tgcatgccag acggttcatt ttgcagcgcc agagtcagaa cttcctcaat
    28801 gcgcttcaca ggatgaatgt ccagatcggc aattacgttg tcaggaatct cttccagatc
    28861 gcgtttattt tcgaacggaa ttagcactgt tttaatcccg ccgcgatgcg ctgccaggag
    28921 tttttctttc aaaccaccga tcggcagtac ctgaccacgc agagtgatct caccggtcat
    28981 tgccacatcg gcacgaaccg ggttaccggt caggcaagaa accagcgcgg tgcacatagc
    29041 aataccggca ctcggaccat ctttcggcgt cgcaccttcc ggtacgtgga cgtggatgtc
    29101 acgtttttcg taaaaatcag ggttgatccc cagtttttcc gcacgcgcac gaaccaccgt
    29161 taacgccgcc tgaatagact cctgcatcac ttcgccgagc gaaccggtat aggtcagttt
    29221 gcctttaccc ggaacacatg cggtttcaat ggtcagcaag tcaccgccca cttccgtcca
    29281 cgccagaccg gttacctgac cgacacggtt ttcgttatca gcgcgaccat agtcgaaacg
    29341 ctgaacaccg aggtagtcat gcaggttatc gccgttaatt tcgatatgtt ttaatgactt
    29401 atcgagcagt aactgcttaa ccgctttgcg gcacagtttg gagatttcac gctccagacc
    29461 acgcacgccc gcctcacggg tgtagtaacg aataatgccg ataatggcgc tatcgtcgac
    29521 ggtcagctca ccttttttca gtgcattacg ttcaatctgc ttcggcagca ggtgacgttt
    29581 ggcgatgttc agtttttcat cttcggtata accggagagg cgaatcactt ccatacgatc
    29641 cagcagcggt gccggaatgt tcatggagtt cgacgtcgcg acaaacatca cgtcgctgag
    29701 atcgtaatcc acttccaggt agtggtcgct gaacgctacg ttctgctctg gatccagcac
    29761 ttcaagcagt gcagaggccg gatcgcctcg catgtcagaa gacattttgt cgatctcatc
    29821 gagcaggaac agcgggtttt tcacgcccac tttcgccatt ttctggatca gtttacccgg
    29881 catagaaccg atgtaagtac ggcggtgacc acggatttcc gcttcatcac gcacgccgcc
    29941 cagcgccata cggacatatt tacgcccggt ggctttggca atggactgac caagagaggt
    30001 tttacctacc cccggcggcc ctaccaggca gaggatcggt cccttgattt tgttgacacg
    30061 gctttgaacc gcaaggtact caaggatgcg atctttcacg cgctccagac cataatggtc
    30121 ggtatcaagg atttcctgcg cctgacgcag gtcttttttg accttgctgc gcgcattcca
    30181 cggtacctgt accatccagt cgatataacc acgcactacg gtcgcttctg ccgacatcgg
    30241 agacatcatt ttcagcttct gcaactctgc ttccgctttc tcttttgcct ctttcggcat
    30301 tttcgccgcg tcgattttgc gcttcagggc ttcgttttcg tccggcgcgt cgtccatttc
    30361 accgagttct ttctgaatag ctttcatttg ctcgttcaga tagtactcac gctgggattt
    30421 ctccatctgc tttttaacgc ggttgcgaat gcgtttctca acctgcagca gatcgatttc
    30481 cgattccatc attgccatca gatattccag acgttcgtta acgtcggaca tctccagaac
    30541 ggactgtttg tcagccagtt tcagcggcat atgtgcagca atggtatccg ccagacgcgc
    30601 cggatcgtcg atgctattca gcgacgtcag cacttctggt gggatttttt tgttcagctt
    30661 gatgtagcct tcgaactggc tgattgcagt acgcaccagc acttcctgtt cccgctcatc
    30721 aatggtcggc gactccagat actccgcctt cgcagaaaag tgttcgccat tgtcagagag
    30781 cgcagaaata cgcgcgcgct gtaacccctc gaccagcact ttgacggtgc cgtcaggcag
    30841 tttcagcatc tgcaatatag aggccacggt cccgacggtg aaaagatcgt ttacacccgg
    30901 ctcatccgtt gaagcttctt tctgcgcgac cagcataatt tttttatcat ggtccatcgc
    30961 cgcttccaga caacggatag atttttcccg cccgacaaat aaggggatga ccatgtgcgg
    31021 ataaaccacc acatcgcgca gcggcaatac ggggatttca atgcgttcag aacgctcagg
    31081 attcatagag ctctctctta gtttaatttc cgccaggtaa tcagatgaca cgactgtgct
    31141 tcacgccatc tattaacatg tacgtcagta tatggggatg tttcccccac attcaacgcc
    31201 gagaatagag gaaaaattaa aggggagata aaatcccccc tttttggtta actaattgta
    31261 tgggaatggt taattattca ccagatgcct gttgcgcttc cggcttacca taaatcagca
    31321 acggtttgct ttgaccatca attaccgact cgtcgataac cactttttcg acatcttcca
    31381 tggacggcag atcgtacatg gtatcgagca gtgcggcttc tacgatggaa cgcaggccac
    31441 gggcaccggt tttacgcgcc atcgctttct tagcgatagc atccagcgcc tcgtcacgga
    31501 attccagatc cacgccttcc agattaaaca gcgcctgata ctgcttggtc agggcgtttt
    31561 tcggctcttt gaggatctga atcagagctt cttcgctcag ttcattcaac gttgcgacaa
    31621 ccggcagacg accaataaac tcagggataa gaccaaactt gatcagatct tccggttcaa
    31681 cctgcgccag cagctcgcct tcgcttgctt tgtcggactt cgcttttacc gtcgcgccaa
    31741 aaccaatgcc ggagccggtt tctacacggt gggaaatcac tttatccaga ccggcaaacg
    31801 caccgccaca aataaacagg atcttagagg tatcaacctg caagaattcc tgctgcggat
    31861 gtttacgccc accttgcggt ggaacagcag ctaccgtacc ttcgatcagt ttcaacagtg
    31921 cctgctgtac gccttcaccg gaaacgtctc gggtaatgga cgggttgtct gacttacgag
    31981 aaatcttgtc gatctcatcg atgtagacaa tcccgcgctg cgctttctgt acgtcgtaat
    32041 cgcacttctg caacagcttc tgaatgatgt tttcaacgtc ttcgcccaca taaccggctt
    32101 cggtcagcgt ggttgcgtcg gccatggtga acgggacgtc cagcaggcgc gccagcgttt
    32161 cagccagcag cgttttaccg gaaccggtcg gaccgatcag cagaatgtta cttttgccca
    32221 actcgacacc attgctggta tcgccgttgc gcagacgttt gtaatggttg tataccgcga
    32281 ccgccagcac ttttttcgcc tgttcctggc cgataacgta atcgtccagg tggttgcgaa
    32341 tttcatgcgg cgtcggtagc gcactgcgtt cacgatgcgg tgcaacttct ttaatctctt
    32401 cgcgaatgat gtcgttacat aaatcaacac attcgtcgca gatatacacg gatggaccgg
    32461 caatcagctt gcgcacttca tgctggcttt tgccgcaaaa agagcaatac agcaatttgc
    32521 ctgagccatc tttgcgttta tctgtcatgg gtcaaaacct cttctttgtt ctttgtgccg
    32581 cacacgacga cgcaaatgcc attctcaggc gcaagccgct tacagcgttg tgccgccctg
    32641 gataagtata gcggcacagt tgcgcctctg gcatcaatta cgatgggtca gaatcgaatc
    32701 gaccagaccg tattccaccg cttcaggggc ggaaaggaag cgatcgcgct cggtatcacg
    32761 ttcaatctgt tctaatgatt gacccgtatg aagcgccata agttcattca tgcgcccttt
    32821 aactttcaga atttcacggg catgaatttc gatatcggtc gcctggccct ggtagccgcc
    32881 caacggttgg tgaatcatca cgcgcgaatt cggcaggcaa aaacgtttac cttttgcccc
    32941 tgcggtcaac aagaaagcgc ccatcgaggc cgcctggccc atacagatgg tgctgacatc
    33001 aggcttgata aactgcatgg tgtcatagat agacatcccg gcagtgatca ccccgcctgg
    33061 ggagttaatg tacagataga tatctttttc tgggttttcc gcttccagga acagcatctg
    33121 cgccacaatc aggttagcca tgtggtcttc aacctggcca gtcagaaaaa tgacgcgttc
    33181 cttaagtaga cgagaataga tatcaaaaga gcgctcaccg cgtgaggtct gttcaatgac
    33241 catcggcacc agcgccatat ggggtgcaaa gttatctcgt tcgccgctgt atgacatttc
    33301 cgtctcctgg ataaaattga aaaaacctgc tgtaccgatt gtaaccttat ggacggatta
    33361 tcagctagtc cctgtgttat gggtacggca tcaccatatt tcaagcataa caatcttttg
    33421 ttgttacgct aacaccgaaa gaggcttttc gcacggttcc atgcaaaatt catgacaaaa
    33481 aaaaagccca ccacctggcg gtgacgggcc tttgtgcgaa tttagcgcgt tatgctgcgt
    33541 aaattacgcc tgctggttca tcagctcgtt gaaagtggtt tctttttcag tcactttcgc
    33601 tttcgccagt acagcttcaa cagcctgttc ttccagagca acattgcgca tgttgtccat
    33661 cagttctttg tttttgctgt agaactcgat aacttctttc ggatcttcgt acgcagaagc
    33721 catctcttcg atcaggcctt tcacgcgctc ttcgtcagct ttcagctcgt tggtgcggat
    33781 aacttcgccc agcagcaggc caacaactac gcggcgttta gcctgttctt cgaacagttc
    33841 gcgcggcagt tccagagctt gtttttcgtt gccaccgaaa cgctgtgcag cctggcgacg
    33901 cagaacgtcg atttcgctgt cgatcagcgc agccggtacg tcgatgtcgt tagcttttac
    33961 cagaccttcg atcgcctgag acttaacgcg gttacggatg gcgctcttca gctcgcgctc
    34021 catgttttta cgcacttcag cgcgcagacc ttctacggaa ccatcttcaa cgccgaaacg
    34081 tttgatgaat tctgcagtca gttccggcag ttcacgctct tcaactttct tcaggttgat
    34141 agcgaatttc gctgctttac ctttcaggtt ttctgcgtgg tattcttccg ggaaggtcac
    34201 gtcgatggtg aactcttcgc cagctttgtg gcctttgata ccgtcttcaa agcccgggat
    34261 catacgaccc tggcccatcg ccagtacgaa atcagacgct ttaccgcctt cgaactcttc
    34321 gccgtctaca gaaccggtga agtcgatggt tacgcggtct tctgcttcaa cagcgccgtc
    34381 tttttctttc caggtcgcct gctgtttacg cagagtatcc agcatgccgt caacgtcagc
    34441 gtcggtcact tcaacgatcg gtttttcaac ttcgatcgct tccagaccct gcagttcaac
    34501 ttccggataa acttcaaact ctacagagta agtgaagtct tcacccagct tgtattcgcc
    34561 cggaacataa gtcggtgcgc cagccggatt gattttttct ttaatgatgg cgtcaatgaa
    34621 gttacggctc atcaggtcac ccagaacgtc ctggcgtaca gacgcgccat aacgctgagc
    34681 aacgatattc attggcactt tgcctttgcg gaagccgtca atacgtactt ttttcgcaac
    34741 gttgaccagc tcgcttttaa cagcggtctc gatgctgtca gcagcgatag taatcgttac
    34801 acggcggcca aggccttgag tggtttcaac tgaaacttgc atcttgttac ctcaaaaaat
    34861 cacagtgctc ggtcaactct taccccgcaa gcacaggttg ttggcttcgc ggaaccggga
    34921 tgttcttata atcaatcaca ttccctgtta ccagaatcat cccgaaagca ttcaaatagg
    34981 acgcggcatt atagcggcat cacttttatg agtcgagaac ggttattgcg cgctgtcgcg
    35041 tattttttca caattcccgg gcaattttcg tctgaatgct gggcaaaaag cgcaaaatcc
    35101 agacaaaaca aaacggcccg caggccgttt ttcataaaat ctttagcaac atactggaaa
    35161 agcgccgaca gttgcaaatg cgtttttacg cgatgcttcc tgctccacga cagggaggag
    35221 aggcaaagac ggtgtcctgc aacccttccc actccttaat agtgtaagta tgcagagcca
    35281 gcgcatgaac ggtagtagag agttcctccg ctaaagtact gtaaatcatt cgatgacgat
    35341 tcagaaaacg ttcacccgta aaacgatcgc tgaccagcac aactttaaaa tggctttcag
    35401 agccggctgg gacattgtga cgatagcttt catccactac ttcgaggaat acgggttgga
    35461 acgccgccct taatttttct tctatccgct cacgtatcat catgaaatat ctcctccgac
    35521 aacgctgaga tgtcatccat ccctttaaat actagccgct tttaccgttt ccattgcagc
    35581 ttaacaacaa atatttaggt ttcgtcctgg tttttccgtc aagcgtatga agtttactga
    35641 tttaaagatt gaaacacagg cctcgcatca gcgtttcttt taccaggggt gattatttcg
    35701 tcaacatgat gaatttacat gccttaccca cttcccctcg ccgttggcaa tgttatgatg
    35761 gcgggaattt ttaccaaaca cgcttaagag tctctgagag cccgaacatg ttcaaaaaaa
    35821 tcctcttccc gttagttgct ctgtttatgc ttgcaggatg cgcaaaaccg ccaacaacta
    35881 ttgaagtttc cccgacgatt acactgccac agcaggatcc aagcctgatg ggcgtcaccg
    35941 taagcattaa tggtgccgat cagcgtaccg atcaggcgct ggcaaaagtc acccgcgata
    36001 atcaaatcgt taccctgacc gcctcccgcg atctgcgttt cctgctgcaa gaagtgctgg
    36061 aaaaacagat gaccgcgcgt ggttacatgg ttggtccgaa tggcccggtt aatctgcaaa
    36121 tcatcgttag ccaactgtat gctgacgtgt cccagggcaa cgtgcgctac aacatcgcta
    36181 ccaaagcgga tatcgccatc atcgctaccg cgcagaatgg caacaaaatg accaaaaact
    36241 atcgtgccag ctacaatgtt gaaggtgcgt tccaggcctc caacaaaaat atcgccgatg
    36301 cggttaacag tgtgctgacc gataccatcg ctgatatgtc tcaggacacc agcattcacg
    36361 aattcatcaa gcagaacgcg cgttaatttc tgccctctgg cccggtgcaa gccgggcctg
    36421 tagacgccca tgtccagtca atatttacgt atttttcaac agccgcgttc agccatattg
    36481 ctgatcctgg gttttgcttc cgggctaccg ctcgccctga catccggcac cttacaggcc
    36541 tggatgacgg tcgagaatat tgatctcaaa accattggtt tcttctctct ggtaggccag
    36601 gcttacgttt ttaaattcct ctggtcaccg ctgatggacc gctacacgcc cccatttttt
    36661 gggcggcggc gcggttggct gctcgccacg caaatcctgt tattagtcgc cattgcggcg
    36721 atgggttttc tcgaacctgg cacccaactc cgctggatgg cggcactggc ggtggtgatc
    36781 gctttttgct cggcctccca ggatattgtc ttcgatgcgt ggaaaaccga tgtgctcccg
    36841 gcagaagaac gtggtgcggg cgcggcaatc agcgtgctgg gttaccgttt agggatgctg
    36901 gtttccggcg gcttagccct gtggctggca gataaatggc tgggctggca gggcatgtac
    36961 tggttgatgg cggcgctgtt gatcccttgt attatcgcga cgttgcttgc accagaaccg
    37021 accgacacca ttcctgtgcc aaaaacgctg gaacaagcgg ttgttgcacc tctgcgagat
    37081 ttctttggtc gcaataatgc ctggcttatt ttgcttctta tcgtgctgta taagctgggc
    37141 gacgcattcg ccatgagcct gacaaccacg tttttgattc gcggcgtcgg gtttgatgcg
    37201 ggtgaagtag gcgtggttaa caaaacgctt ggcttactgg cgaccattgt gggcgcattg
    37261 tacggtggga ttttgatgca gcgcctgtcg ctgttccggg cgctgctgat tttcggcatt
    37321 ttacaaggtg cgtctaacgc tggttactgg ctgctgtcga ttactgataa gcatctctac
    37381 agcatgggcg cagccgtctt tttcgaaaac ctctgtggcg ggatgggcac atcagccttt
    37441 gtcgcgctgt taatgacgct gtgtaataag tcattttccg caactcaatt tgccctgctc
    37501 tcagcgcttt ccgctgtagg acgagtttat gtcggccccg tggcgggttg gtttgttgaa
    37561 gcacacggct ggtcgacatt ctatctattc tccgtcgccg ctgccgtacc ggggcttatt
    37621 ttgctgctgg tttgccgcca gacgcttgaa tatacaggag taaatgacaa ctttatctcc
    37681 cgtaccgaat atccggcagg ttatgccttt gccatgtgga cactggcggc gggcgtcagc
    37741 ctgttggccg tgtggttact gctgttgacg atggacgcgc tggatttgac gcacttctct
    37801 ttcctgcctg ctctgctgga agtcggggtt ttagtcgccc tttctggcgt cgtgcttggt
    37861 ggtttgctgg attatctggc gctacgaaaa acgcatctga cgtaatctgt aaatattatt
    37921 tagctgtagt aatcactcgc cgtaattatt acggctaaat aaacatcagt atcgcttaat
    37981 aatatttgtt cttttgtgcg gcttagcgtt tggaaattat tgacgcaatt tatgaattct
    38041 attttttctt acacgattca gctaatgagt ctttattttc ccccacccaa ttgtcaccat
    38101 aatgataatt atttgttaaa caattgtttt attccagatt ggttatacca attgcccgtc
    38161 caaactcctt ctgcactccc ctttcgttat aacgcccttt tgcaacagct tcttaaaatc
    38221 aacttgatat gttttgcaac atatgtgacc tgacagccaa atccaagtaa caggaattta
    38281 atcatgttta cagtaatgta accttcccgt aaaatgccca cacactttaa acgccaccag
    38341 atcccgtgga attgaggtcg ttaaatgaga ctcaggaaat acaataaaag tttgggatgg
    38401 ttgtcattat ttgcaggcac tgtattgctc agtggctgta attctgcgct gttagatccc
    38461 aaaggacaga ttggtctgga gcaacgttca ctgatactga cggcatttgg cctgatgttg
    38521 attgtcgtta ttcccgcaat cttgatggct gttggtttcg cctggaagta tcgtgcgagc
    38581 aataaagatg ctaagtacag cccgaactgg tcacactcca ataaagtgga agctgtggtc
    38641 tggacggtac ctatcttaat catcatcttc cttgcggtac tgacctggaa aaccactcac
    38701 gctcttgagc ctagcaagcc gctggcacac gacgagaagc ccattaccat cgaagtggtt
    38761 tccatggact ggaaatggtt cttcatctac ccggaacagg gcattgctac cgtgaatgaa
    38821 atcgctttcc cggcgaacac tccggtgtac ttcaaagtga cctccaactc cgtgatgaac
    38881 tccttcttca ttccgcgtct gggtagccag atttatgcca tggccggtat gcagactcgc
    38941 ctgcatctga tcgccaacga acccggtact tatgacggta tctccgccag ctacagcggg
    39001 ccgggcttct caggcatgaa gttcaaagct attgcaacac cggatcgcgc cgaattcgac
    39061 caatgggtcg caaaagcgaa acagtcgccg aacaccatgt ctgacatggc tgcgttcgaa
    39121 aaactggccg cgcctagcga atacaaccag gtggaatatt tctccaacgt gaaaccagac
    39181 ttgtttgccg atgtgattaa caagtttatg gctcacggta agagcatgga catgacccag
    39241 ccagaaggtg agcacagtgc acacgaaggt atggaaggca tggacatgag ccacgcggaa
    39301 tccgcccatt aaaggggttg aggaagaata aagatgttcg gaaaattatc acttgatgca
    39361 gtcccgttcc atgaacctat cgtcatggtt acgatcgctg gcattatttt gggaggtctg
    39421 gcgctcgttg gcctgatcac ttacttcggt aagtggacct acctgtggaa agagtggctg
    39481 acctccgtcg accataaacg cctcggtatc atgtatatca tcgtggcgat tgtgatgttg
    39541 ctgcgtggtt ttgctgacgc cattatgatg cgtagccagc aggctcttgc ctcggcgggc
    39601 gaagcgggct tcctgccacc tcaccactac gatcagatct tcaccgcgca cggcgtgatt
    39661 atgatcttct tcgtggcgat gcctttcgtt atcggtctga tgaacctggt ggttccgctg
    39721 cagatcggcg cgcgtgacgt tgcgttcccg ttcctcaaca acttaagctt ctggtttact
    39781 gttgttggtg tgattctggt taacgtttct ctcggcgtgg gcgaatttgc gcagaccggc
    39841 tggctggcct atccaccgct atcgggaata gagtacagtc cgggagtcgg tgtcgattac
    39901 tggatatgga gtctccagct atccggtata ggtacgacgc ttaccggtat caacttcttc
    39961 gttaccattc tgaagatgcg cgcaccgggc atgaccatgt tcaagatgcc agtatttacc
    40021 tgggcatcac tgtgcgcgaa cgtactgatt attgcttcct tcccaattct gacggttacc
    40081 gtcgcgttgt tgaccctgga tcgctatctg ggcacccatt tctttaccaa cgatatgggt
    40141 ggcaacatga tgatgtacat caacctgatt tgggcctggg gccacccgga agtttacatc
    40201 ctgatcctgc ctgttttcgg tgtgttctcc gaaattgcgg caaccttctc gcgtaaacgt
    40261 ctgtttggtt atacctcgct ggtatgggca accgtctgta tcaccgtgct gtcgttcatc
    40321 gtttggctgc accacttctt tacgatgggt gcgggcgcga acgtaaacgc cttctttggt
    40381 atcaccacaa tgattatcgc catcccgacc ggggtgaaga tcttcaactg gctgttcacc
    40441 atgtatcagg gccgcatcgt gttccattct gcgatgctgt ggaccatcgg ttttatcgtc
    40501 actttctcgg tgggcgggat gactggcgtg ctgctggcag taccgggcgc ggacttcgtt
    40561 ctgcataaca gcctgttcct gattgcgcac ttccataacg tgatcatcgg cggcgtggtc
    40621 ttcggctgct tcgcagggat gacctactgg tggcctaaag ccttcggttt caaactgaat
    40681 gaaacctggg gtaaacgcgc gttctggttc tggatcatcg gcttcttcgt tgcctttatg
    40741 ccgctgtatg cgctgggctt catgggcatg acccgtcgtt tgagccagca gattgacccg
    40801 cagttccaca ccatgctgat gattgcagct agcggtgcag tactgattgc gctgggtatt
    40861 ctctgcctcg ttattcagat gtacgtttct attcgcgacc gcgaccagaa ccgtgacctg
    40921 actggcgacc cgtggggtgg ccgtacgctg gagtgggcaa cctcttcccc gcctccgttc
    40981 tataactttg ccgttgtgcc gcacgttcac gagcgtgatg cattctggga aatgaaagag
    41041 aaaggcgaag cgtataaaaa gcctgaccac tatgaagaaa ttcatatgcc gaaaaacagc
    41101 ggtgcaggta tcgtcattgc agctttctcc accatcttcg gtttcgccat gatctggcat
    41161 atctggtggc tggcgattgt tggcttcgca ggcatgatca tcacctggat cgtgaaaagc
    41221 ttcgacgagg acgtggatta ctacgtgccg gtggcagaaa tcgaaaaact ggaaaaccag
    41281 catttcgatg agattactaa ggcagggctg aaaaatggca actgatactt tgacgcacgc
    41341 gactgcccac gcgcacgaac acgggcacca cgatgcaggc ggaaccaaaa tcttcggatt
    41401 ttggatctac ctgatgagcg actgcattct gttctcaatc ttgtttgcta cctatgccgt
    41461 tctggtgaac ggcaccgcag gcggcccgac aggtaaggac attttcgaac tgccgttcgt
    41521 tctggttgaa actttcttgc tgttgttcag ctccatcacc tacggcatgg cggctatcgc
    41581 catgtacaaa aacaacaaaa gccaggttat ctcctggctg gcgttgacct ggttgtttgg
    41641 tgccggattt atcgggatgg aaatctacga attccatcac ctgattgtta acggcatggg
    41701 tccggatcgc agcggcttcc tgtcagcgtt ctttgcgctg gtcggcacgc acggtctgca
    41761 cgtcacttcc ggtcttatct ggatggcggt gctgatggta caaatcgctc gtcgcggcct
    41821 gaccagcact aaccgtaccc gcatcatgtg cctgagcctg ttctggcact tcctggatgt
    41881 ggtttggatc tgtgtgttca ctgttgttta tctgatgggg gcgatgtaat gagtcattct
    41941 accgatcaca gcggcgcgtc ccatggcagc gtaaaaacct acatgacagg ctttatcctg
    42001 tcgatcattc tgacggtgat tccgttctgg atggtgatga caggggctgc ctctccggcc
    42061 gtaattctgg gaacaatcct ggcaatggca gtggtacagg ttctggtgca tctggtgtgc
    42121 ttcctgcaca tgaataccaa atcagatgaa ggctggaaca tgacggcgtt tgtcttcacc
    42181 gtgctaatca tcgctatcct ggttgtaggc tccatctgga ttatgtggaa cctcaactac
    42241 aacatgatga tgcactaaga gcggcggtta tgatgtttaa gcaatacctg caagtaacga
    42301 aaccaggcat catctttggc aacctgatct cggtgattgg gggattcctg ctggcctcaa
    42361 agggcagcat tgattatccc ctgtttatct acacgctggt tggggtgtca ctggttgtgg
    42421 cgtcgggttg tgtgtttaac aactacatcg acagggatat cgacagaaag atggaaagga
    42481 cgaagaatcg ggtgctggtt aaaggcctga tctctcctgc tgtctcgctg gtgtacgcca
    42541 ccttgctggg tattgctggc tttatgctgc tgtggtttgg cgcgaatccg ctggcctgct
    42601 ggctgggggt gatgggcttt gtggtttatg tcggcgtcta tagcctgtac atgaaacgcc
    42661 actcagtcta cggcacattg attggttcgc tctccggcgc tgcgccgccg gtgattggct
    42721 actgtgcagt aaccggcgag ttcgatagcg gcgcagcgat cctgctggct atcttcagcc
    42781 tgtggcagat gcctcactcc tatgccatcg ccattttccg ctttaaggat taccaggcgg
    42841 caaacattcc ggtattgcca gtggtaaaag gcatttcggt ggcgaagaat cacatcacgc
    42901 tgtatatcat cgcctttgcc gttgccacgc tgatgctctc tcttggcggt tacgctggat
    42961 ataaatatct ggtggtcgcc gcggcggtta gcgtctggtg gttaggtatg gctctgcgcg
    43021 gttataaagt tgctgatgac agaatctggg cgcgcaagct gttcggcttc tctatcatcg
    43081 ccatcactgc cctctcggtg atgatgtccg ttgattttat ggtaccggac tcgcatacgc
    43141 tgctggctgc tgtgtggtaa caaaacctct ctattaaaaa ggtgctacgg cacctttttt
    43201 attgtctgag atcagagata cggctaataa ccgtatgtct tgacagagtg agcagatgaa
    43261 cataaattaa tcatacggtt gatagccgta ttatgtatga gatcgactcc ctacgatttc
    43321 attcagttaa tcaaagaggt cataatgcct ggaaaaacag ccactctacc cgatgtcgat
    43381 aaaacgctga aaaatgcgcg ggtcgaactc aaaactagtc ccgacgccaa aaacaaactt
    43441 cgtgaagctg cgcaagccgt tggggtagat ttaagcgctt ttattttgag cgctgctatg
    43501 gaacgcgccg aaagtgtgct tgataaccaa cgtcgccgtg agctttcgaa tcaaagctgg
    43561 gaactgatga accaactcat cgctgaacct gctcaaccga cgctcgccct caaggcgtta
    43621 atgaaaagga aaaacagcga tggtcgacaa gcatgaagag attactctgc ccatagtcct
    43681 ctcctgtaat tatcagtctg atattactta tcctgggcaa aaacagtttg attgcggtaa
    43741 ccctgttatc gataaatttg tacgcgcatc gctaaagaaa agtgtgcgta atagcgactg
    43801 tgcggctaaa gcacttattg acagacaaag tggtgaactg atcggcatct gtacttttac
    43861 ggcatattcg ctggaaaaac aacgcgtttc tggcgtcctt cagggttcac aaccttcaga
    43921 aattggtgtt gtcagattag tcatgttggg ggtagcacgg aagtatcaaa agcggggctt
    43981 tggtcaggac ctactatgtg atttttttga acatgtaaaa ataattcacc aggcattacc
    44041 aattaaaggg gtttatcttg atgctgaccc tgccgccatt aatttttatg ctcgtctcgg
    44101 ctttgttcag ctttcagcga caccaaatgc ttttggtgct gtacctatgt ttttggcgat
    44161 tcagcatatt ctcgcggctt agattattat cgccacccgc cagggcaagt tttgcctcag
    44221 ccgggggctg agcagcgtta ccacttttgt tcaattgccc ccgctttcag caactccaca
    44281 tccttgccgt taatggttgc cgttgcggca acgattgtgg tccttacagt tgcacaagat
    44341 tttcgctcaa attccggttt ataaagaata agagacgaat tactggtaaa tgaaccaaat
    44401 aggtaatcag taaagctaat attcagccgg tttaaaccga aaggcgcgaa gccaaccgtt
    44461 tggttaatct ccttaccgga tgggcgacga atgctgaatg tcacgcgaag attggccact
    44521 tcgcctaatt tattggtggt ggcgaagagg ctatccatgc aggaaaaacc tttatctaaa
    44581 aaattatctc gcatcccaga acgggtaacc gtaatcacgg gtaacgtatc gtcaaccgtt
    44641 tttctgtcaa tcatgtcttt tcgacaagta gccagaaagt cttctgggcc accataaatg
    44701 tggacacctt tatcaagata ttccttcgct ttcttacaat ctacctcaac atactgacca
    44761 ttggcgtaca taatgcccaa cctggacctt gctaaattca caccgtgctg cgcggcctga
    44821 ttaaatagtg catacgcttt ggcataatcc acccgcacct gctcaccgtc gtagtagata
    44881 ccccccagcg ctaacggagc ttctctgtca ccttgtttag ccactaactc gaaccattta
    44941 atcgcttcct gaacatcagg tttggtatca taacgaggct gataatattc ccagcctaac
    45001 aggctctgcg cgtcgatatc tcccgattca gccattttat gcagtgtatt tataacatcg
    45061 ggagcaggtt taccaccgta tatgctgtca tacaattcat gcgctttctc gctggccacc
    45121 acgcttggtg aaactatcag cattacagaa aataatattc ttttagttaa catgtatacc
    45181 tttatgtc
//
LOCUS       DAZFWL020000023        41939 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFWL020000023 DAZFWL020000000
VERSION     DAZFWL020000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 41939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.023"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(47..1444)
                     /gene="ccp"
                     /locus_tag="ACHKNP_RS07050"
                     /old_locus_tag="pgaptmp_001410"
     CDS             complement(47..1444)
                     /gene="ccp"
                     /locus_tag="ACHKNP_RS07050"
                     /old_locus_tag="pgaptmp_001410"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HGU4703606.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1849..3498
                     /locus_tag="ACHKNP_RS07055"
                     /old_locus_tag="pgaptmp_001411"
     CDS             1849..3498
                     /locus_tag="ACHKNP_RS07055"
                     /old_locus_tag="pgaptmp_001411"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU4703607.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3549..4151)
                     /locus_tag="ACHKNP_RS07060"
                     /old_locus_tag="pgaptmp_001412"
     CDS             complement(3549..4151)
                     /locus_tag="ACHKNP_RS07060"
                     /old_locus_tag="pgaptmp_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGU4703608.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4671..5570
                     /locus_tag="ACHKNP_RS07065"
                     /old_locus_tag="pgaptmp_001413"
     CDS             4671..5570
                     /locus_tag="ACHKNP_RS07065"
                     /old_locus_tag="pgaptmp_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4703609.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5619..6632
                     /gene="yhjD"
                     /locus_tag="ACHKNP_RS07070"
                     /old_locus_tag="pgaptmp_001414"
     CDS             5619..6632
                     /gene="yhjD"
                     /locus_tag="ACHKNP_RS07070"
                     /old_locus_tag="pgaptmp_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HGU4703610.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7043..8365
                     /locus_tag="ACHKNP_RS07075"
                     /old_locus_tag="pgaptmp_001415"
     CDS             7043..8365
                     /locus_tag="ACHKNP_RS07075"
                     /old_locus_tag="pgaptmp_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4703611.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8599..10659)
                     /locus_tag="ACHKNP_RS07080"
                     /old_locus_tag="pgaptmp_001416"
     CDS             complement(8599..10659)
                     /locus_tag="ACHKNP_RS07080"
                     /old_locus_tag="pgaptmp_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU4703612.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10729..11496)
                     /gene="pdeH"
                     /locus_tag="ACHKNP_RS07085"
                     /old_locus_tag="pgaptmp_001417"
     CDS             complement(10729..11496)
                     /gene="pdeH"
                     /locus_tag="ACHKNP_RS07085"
                     /old_locus_tag="pgaptmp_001417"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HGU4703613.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11728..12657
                     /gene="kdgK"
                     /locus_tag="ACHKNP_RS07090"
                     /old_locus_tag="pgaptmp_001418"
     CDS             11728..12657
                     /gene="kdgK"
                     /locus_tag="ACHKNP_RS07090"
                     /old_locus_tag="pgaptmp_001418"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HGU4703614.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12753..14249)
                     /locus_tag="ACHKNP_RS07095"
                     /old_locus_tag="pgaptmp_001419"
     CDS             complement(12753..14249)
                     /locus_tag="ACHKNP_RS07095"
                     /old_locus_tag="pgaptmp_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU4703615.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(14469..15755)
                     /gene="dctA"
                     /locus_tag="ACHKNP_RS07100"
                     /old_locus_tag="pgaptmp_001420"
     CDS             complement(14469..15755)
                     /gene="dctA"
                     /locus_tag="ACHKNP_RS07100"
                     /old_locus_tag="pgaptmp_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HGU4703616.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15938..17926)
                     /gene="hmsP"
                     /locus_tag="ACHKNP_RS07105"
                     /old_locus_tag="pgaptmp_001421"
     CDS             complement(15938..17926)
                     /gene="hmsP"
                     /locus_tag="ACHKNP_RS07105"
                     /old_locus_tag="pgaptmp_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HGU4703617.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSMLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18008..21481)
                     /gene="bcsC"
                     /locus_tag="ACHKNP_RS07110"
                     /old_locus_tag="pgaptmp_001422"
     CDS             complement(18008..21481)
                     /gene="bcsC"
                     /locus_tag="ACHKNP_RS07110"
                     /old_locus_tag="pgaptmp_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HGU4703618.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTLEPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21463..22569)
                     /gene="bcsZ"
                     /locus_tag="ACHKNP_RS07115"
                     /old_locus_tag="pgaptmp_001423"
     CDS             complement(21463..22569)
                     /gene="bcsZ"
                     /locus_tag="ACHKNP_RS07115"
                     /old_locus_tag="pgaptmp_001423"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HGU4703619.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDIWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22576..24915)
                     /gene="bcsB"
                     /locus_tag="ACHKNP_RS07120"
                     /old_locus_tag="pgaptmp_001424"
     CDS             complement(22576..24915)
                     /gene="bcsB"
                     /locus_tag="ACHKNP_RS07120"
                     /old_locus_tag="pgaptmp_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HGU4703620.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24926..27544)
                     /gene="bcsA"
                     /locus_tag="ACHKNP_RS07125"
                     /old_locus_tag="pgaptmp_001425"
     CDS             complement(24926..27544)
                     /gene="bcsA"
                     /locus_tag="ACHKNP_RS07125"
                     /old_locus_tag="pgaptmp_001425"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HGU4703621.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27541..28293)
                     /gene="bcsQ"
                     /locus_tag="ACHKNP_RS07130"
                     /old_locus_tag="pgaptmp_001426"
     CDS             complement(27541..28293)
                     /gene="bcsQ"
                     /locus_tag="ACHKNP_RS07130"
                     /old_locus_tag="pgaptmp_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HGU4703622.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(28305..28493)
                     /gene="bcsR"
                     /locus_tag="ACHKNP_RS07135"
                     /old_locus_tag="pgaptmp_001427"
     CDS             complement(28305..28493)
                     /gene="bcsR"
                     /locus_tag="ACHKNP_RS07135"
                     /old_locus_tag="pgaptmp_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HGU4703623.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            28766..30337
                     /gene="bcsE"
                     /locus_tag="ACHKNP_RS07140"
                     /old_locus_tag="pgaptmp_001428"
     CDS             28766..30337
                     /gene="bcsE"
                     /locus_tag="ACHKNP_RS07140"
                     /old_locus_tag="pgaptmp_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HGU4703624.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            30334..30525
                     /gene="bcsF"
                     /locus_tag="ACHKNP_RS07145"
                     /old_locus_tag="pgaptmp_001429"
     CDS             30334..30525
                     /gene="bcsF"
                     /locus_tag="ACHKNP_RS07145"
                     /old_locus_tag="pgaptmp_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HGU4703625.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            30522..32201
                     /gene="bcsG"
                     /locus_tag="ACHKNP_RS07150"
                     /old_locus_tag="pgaptmp_001430"
     CDS             30522..32201
                     /gene="bcsG"
                     /locus_tag="ACHKNP_RS07150"
                     /old_locus_tag="pgaptmp_001430"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HGU4703626.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVSDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(32288..32395)
                     /locus_tag="ACHKNP_RS07155"
                     /old_locus_tag="pgaptmp_001431"
     CDS             complement(32288..32395)
                     /locus_tag="ACHKNP_RS07155"
                     /old_locus_tag="pgaptmp_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4703627.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(32771..32878)
                     /locus_tag="ACHKNP_RS07160"
                     /old_locus_tag="pgaptmp_001432"
     CDS             complement(32771..32878)
                     /locus_tag="ACHKNP_RS07160"
                     /old_locus_tag="pgaptmp_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4703628.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(33254..33361)
                     /locus_tag="ACHKNP_RS07165"
                     /old_locus_tag="pgaptmp_001433"
     CDS             complement(33254..33361)
                     /locus_tag="ACHKNP_RS07165"
                     /old_locus_tag="pgaptmp_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4703629.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(33737..33844)
                     /locus_tag="ACHKNP_RS07170"
                     /old_locus_tag="pgaptmp_001434"
     CDS             complement(33737..33844)
                     /locus_tag="ACHKNP_RS07170"
                     /old_locus_tag="pgaptmp_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4703630.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            34320..35591
                     /locus_tag="ACHKNP_RS07175"
                     /old_locus_tag="pgaptmp_001435"
     CDS             34320..35591
                     /locus_tag="ACHKNP_RS07175"
                     /old_locus_tag="pgaptmp_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HGU4703631.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(35621..36625)
                     /gene="dppF"
                     /locus_tag="ACHKNP_RS07180"
                     /old_locus_tag="pgaptmp_001436"
     CDS             complement(35621..36625)
                     /gene="dppF"
                     /locus_tag="ACHKNP_RS07180"
                     /old_locus_tag="pgaptmp_001436"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HGU4703632.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(36622..37605)
                     /gene="dppD"
                     /locus_tag="ACHKNP_RS07185"
                     /old_locus_tag="pgaptmp_001437"
     CDS             complement(36622..37605)
                     /gene="dppD"
                     /locus_tag="ACHKNP_RS07185"
                     /old_locus_tag="pgaptmp_001437"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HGU4703633.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDIYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(37616..38518)
                     /gene="dppC"
                     /locus_tag="ACHKNP_RS07190"
                     /old_locus_tag="pgaptmp_001438"
     CDS             complement(37616..38518)
                     /gene="dppC"
                     /locus_tag="ACHKNP_RS07190"
                     /old_locus_tag="pgaptmp_001438"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HGU4703634.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(38528..39547)
                     /gene="dppB"
                     /locus_tag="ACHKNP_RS07195"
                     /old_locus_tag="pgaptmp_001439"
     CDS             complement(38528..39547)
                     /gene="dppB"
                     /locus_tag="ACHKNP_RS07195"
                     /old_locus_tag="pgaptmp_001439"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HGU4703635.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(39698..41305)
                     /gene="dppA"
                     /locus_tag="ACHKNP_RS07200"
                     /old_locus_tag="pgaptmp_001440"
     CDS             complement(39698..41305)
                     /gene="dppA"
                     /locus_tag="ACHKNP_RS07200"
                     /old_locus_tag="pgaptmp_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HGU4703636.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(41657..>41939)
                     /locus_tag="ACHKNP_RS07205"
                     /old_locus_tag="pgaptmp_001441"
     CDS             complement(41657..>41939)
                     /locus_tag="ACHKNP_RS07205"
                     /old_locus_tag="pgaptmp_001441"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703637.1"
                     /translation="SVASGNGTALIVGCGATALFDQHTTHFPANFAPHISLSINGFVT
                     ETIVFFYICLNLFLRYLWLALAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         9776 a        11530 c        10586 g        10047 t
ORIGIN      
        1 cgtaataaaa atcgcccaaa cattcctgtt cgggcgatca aattaattat tgtttatcct
       61 gcatatacgg cgtgtacacc ccgttcagac tgtgcaggaa agctacgata tcatccacat
      121 cctcctgcgg cagctctttg cctacctgat agcgcagcat cagtttcacc gccccgtcca
      181 gcgtcggcac gtcaccgcga tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg
      241 gtactttctg acgcaattta tcacgctctt ctttagtcac attcatacga ccaatatccg
      301 ccgccgtaat ttccccaaag ttaaagtctt ttttcagccc caacggttca aaggaacgtc
      361 cgccgagaat aataccacca tgacaagttg cacatttatt atctttaaat aattgatagc
      421 cttttttctg ttgcgccgtc agcgcatttt catctccacg caaccattta tcaaatgggg
      481 aatccggcgt aattaatgtt ttctcaaatt cagcaatggc atcagtaata ttttcgccac
      541 tgaaaccttg cggataaact tcgaggaact gcgctttaag ctgcggatct ttttccagct
      601 tagcaataat ttcgtcccag gatttcgacg ccatttcaat cgggttcaac ggcggtccac
      661 ctgcctgatc ctgcaatgtt gccgcacgac catcccagaa ctgctcaacg ttaaatactg
      721 agttaaatac cgtcggcgcg ttaatcggcc caactgcgcc accaacacca atcgatgttt
      781 ttctgccatc gacgcccccc gcattcaacg catggcaatg agcgcatgaa atggtgctat
      841 cagccgataa acggggatcg tgatacagcg caaagcccaa cgccactttt tgcgcatcgg
      901 taggcagttt ttgcgggatg ggctgcaccg gttcattgcg atgctccgga gcagtatcat
      961 tgctggcgta atattccgcg cgctgttttg caatccaggc cagtatttcc gcccgctctt
     1021 catcactcac cttacccgcc cagtgtagcg cggtataacg cgttggtggc atagtttcat
     1081 actgcatcac ccattcaatc ttattcagat cgctttgcga aacgggttta tcagccagca
     1141 gtgccgcacg cacggcctcg aggttaaaag atttatatcc aagcttaatg tcgtaatcca
     1201 tcaactgttt cgcgccagga atataataat aggcgggtaa ttctgccgaa ggcgtgtggc
     1261 aatagtcgca tcctttttcg cggagaaagc ctaaaacctt attattttca ctgacagcag
     1321 atgcctgaac atcggcctgt ttactgcgtt tattatcgtg gtaccacaca taaccagata
     1381 accctaaata gcaaatcgcg acgccagcca ggccgatcgc ggtaatacgt gagaccattt
     1441 tcattattct tccctcacgg tggtgagtta ttgtgaccca gaaaattttc cagaatgctg
     1501 caaacgtctc tgcgagacaa agccatcata aaaaacaggg agatacgctt tttgatagga
     1561 atcaatcaat tacatgaata taaactatca ataagatagc ctgtgcctat tacccagatc
     1621 aggaatggca tatcatgatt gcggcgagag caggattgaa tgttctgaaa atgaaaaaca
     1681 acacgaaatc cgtattttta taaattccat tgatattagt gcgttatatt ttttgacaag
     1741 ttaataaacg tgatccaccg cacgctttgt cgcccaccag gcggagcgaa tgactaccct
     1801 taaagaaaag cccgataatt agcgacgaat ttcggaggtt ggatccttat gctcaatcag
     1861 aaaattcaaa accctaatcc agacgaactg atgatcgagg tcgatctctg ctatgaactg
     1921 gacccgtatg aattaaaact ggatgagatg atcgaggcag aaccggaacc cgagatgatt
     1981 gaagggctgc ccgcctctga tgcgctgacg cctgccgatc gctatctcga actgttcgag
     2041 catgttcagt cggcgaaaat tttccccgac agtaaaacct ttcccgactg cgcacccaaa
     2101 atggacccgc tggatatttt aatccgctac cgtaaagtgc gccgtcatcg tgattttgac
     2161 ttgcgcaagt ttgttgaaaa tcacttctgg ctgccggagg tctactccag cgagtatgta
     2221 tcggacccgc aaaattccct gaaagagcat atcgaccagc tgtggccggt gctaacccgc
     2281 gaaccacagg atcacattcc gtggtcttct ctgctggcgc taccgcagtc atatattgtc
     2341 ccgggcggcc gttttagcga aacctactat tgggattcct atttcaccat gctggggctg
     2401 gcggaaagtg gtcgggaaga tttgctgaaa tgcatggccg ataacttcgc ctggatgatc
     2461 gaaaactatg gtcacatccc caacggcaac cgcacctatt atttgagccg atcgcaacca
     2521 ccggtttttg cgctgatggt ggagttgttt gaagaagatg gtgtacgcgg tgcgcgccgc
     2581 tatctcgacc accttaaaat ggaatatgcc ttctggatgg acggtgcaga atcgttgatc
     2641 cctaatcagg cctatcgcca tgttgtgcgg atgccggacg gatcgctgct caaccgttat
     2701 tgggacgatc gcgacacgcc gcgtgacgaa tcctggcttg aggacgttga aaccgcgaaa
     2761 cattctggtc gcccgcccaa cgaggtgtac cgcgatttac gcgcgggagc ggcctcaggt
     2821 tgggattact cttcccgttg gctgcgtgat actggtcgtc tggcgagcat tcgtaccacc
     2881 cagttcatcc ccatcgatct gaatgccttc ctgtttaaac tggagagcgc catcgccaac
     2941 atctcggcgc tgaaaggcga gaaagagaca gaagcgctgt tccgccagaa ggccagtgcc
     3001 cgtcgcgatg cggtaaaccg ttacctctgg gatgatgaaa acggcatcta ccgcgattac
     3061 gactggcgac gcgaacaact ggcgctgttt tccgctgccg ccattgtgcc gctctatgtc
     3121 ggcatggcga accatgaaca ggccgatcgt ctggcaaacg ccgtacgcag ccggttactg
     3181 acacctggcg ggattctggc aagcgagtac gaaaccggtg aacagtggga taaacccaat
     3241 ggctgggcac cgttacaatg gatggcaatt cagggattta aaatgtatgg cgatgacctt
     3301 ctgggtgatg aaatcgcgcg cagctggctg aaaacggtga atcagttcta tctggaacag
     3361 cacaaaatga tcgagaaata ccatattgcc gatggtgttc cccgcgaagg cggcggtggc
     3421 gagtatccgt tgcaggatgg gtttggctgg actaacggtg tggtacgccg tttaattggt
     3481 ttgtacggcg aaccataata tttttacagc cagccgctaa cttcctgctg gctgtaaaat
     3541 tatcctcttc aggaggagat atttaacatc attgccgcct gggtgcgatt tttcacttcc
     3601 agacggcgat acagggattc cagatgcgct tttaccgttc cggtactgat attcagcgct
     3661 ctgccgatct ccttatttga ttcgcccgcc gctaacatgg ttaaaatctc ccgctggcgg
     3721 gcgcttaacg atttgagatc tttaatgtcc ttttccggcg tcgtccgcca gtctccaggc
     3781 agaaacatca tccccatcgc cgcactattt accgccaacg caaatgtctc gacggttgaa
     3841 tcacgaggca caatggccag cacattaaaa tggataactt cctgtaacca ccgtttattg
     3901 caatccgtcg ccgtgattaa caccttaacc tcaggaaact gcaccacggt tttttgcagc
     3961 aaccagtagc aaaactcacc atcctgatcg ccatcgagca taactaaggc ttcagggtaa
     4021 ctttccagct tttgccataa ctcgtctgcc tgactggccc cctgaatact cactcctgga
     4081 atacgctgct gtaaactgat tttcattcca tgaataaata ttgactgcct gtcaaacatg
     4141 actatttgca taactgaatc tccacctgaa tacgttaaaa agacttaagt agtggaaggg
     4201 tattacccgc gagaaaaaat aagaattcgc catttggcgg tggccattct acagagatga
     4261 cgcgtagaaa atagttaccg atataaatag ttacagctaa acgcctgaaa ttacatgtcg
     4321 agggcactat ttaaaacaat tttgaggatt tccttatatt ggtggttagt acgcatgcaa
     4381 ttaaaaatga aactccgcga ccacaagcaa aaataacaaa cggcaatgag acaaaaataa
     4441 gcacaaatag ccaacacgtc ctctattcac tttaaaggga atcgctgaaa aatacgctct
     4501 gtttaggggg attcaccttt ctcagaaagc tattccgccc ttttcctgct gagaaatcgc
     4561 cacattcgtc atgacaacat tgtgaaaccc ggcattagat gttagaaaaa acaataaaca
     4621 atgcgatatg cgcgttactg tttcgctgtg aacaacaatc ggtcaaagaa atggataaaa
     4681 ttcacgcaat gcagttgttc atcaaagtcg cggagctgga aagtttttcc cgcgcggcgg
     4741 atttctttgc tttgccaaaa ggaagtgttt cgcgccagat acaggcactg gaacatcaac
     4801 ttggcaccca gcttctccag cgcaccacgc gacgggtcaa actcacgcca gaaggcatga
     4861 cctattatca acgagcaaaa gatgtgttga gtaatctcag cgaactggac ggtctgtttc
     4921 aacaggatgc caccagtatc agcggtaaat tacgcatcga catcccgcca ggaatcgcga
     4981 aaagcctgtt actgccgcgc ctgtcggaat ttctctatct gcatccggga attgagctgg
     5041 aactgagtag ccatgaccgt ccggtagata ttcttcatga tggttttgat tgcgtgatac
     5101 gcactggcgc gttaccggaa gatggcgtta tcgcccgtcc cctcggcaaa ctgaccatgg
     5161 tcaactgtgc cagtccgcac tatctgacgc gctttggtta tccgcaaagc cccgatgatc
     5221 tgacttcaca cgcaatagtg cgttatacac cgcacctggg tgtacatccg ttaggttttg
     5281 aggttgccag cgttaatggc gtccagtggt ttaagtctgg cggcatgttg acggtaaaca
     5341 gtagcgaaaa ctatctcgcc gccggtcttg ccggtctggg gattattcag atcccgcgca
     5401 ttgccgtgcg cgaagccctg cgtgccgggc ggcttattga agtgttgcca ggctaccgtg
     5461 ccgagccgct ctccctttcg ctggtttacc cgcagcgtcg ggagctttcc cggcgtgtaa
     5521 acctgtttat gcagtggctg gctggcgtaa tgaaagagca cctggactga ccgactatac
     5581 tttttaagaa tgaccacgac agaaggacaa aagagcggat gacgcaggaa aacgagatca
     5641 aacgtcccac ccaggatctg gagcacgagc cgattaaaca gttggataat agcgagaaag
     5701 gcggcaaagt tagccaggcg ctggaaaccg tcaccaccac tgccgaaaaa gtccaacgcc
     5761 aaccggttat tgcgcacctg attcgtgcga cagaacgctt taacgatcgg ctgggtaacc
     5821 agtttggtgc ggctatcacc tatttctcat ttttgtcgat gatcccgatt ttgatggtgt
     5881 cgtttgccgc agggggcttt gtgctggcct cccatccgat gttgctacag gatatcttcg
     5941 acaaaattct gcaaaacatc agcgatccga cgctagccgc cacgttgaaa aacaccatca
     6001 acaccgccgt tcagcagcgt acgactgtag ggcttgtcgg cctggcggtg gcactttatt
     6061 ccggcatcaa ctggatgggt aacctgcgtg aagcgattcg tgcccagtcg cgcgatgtct
     6121 gggaacgctc gccgcaagat caggagaagt tctgggtaaa atatctgcgt gattttattt
     6181 cactgattgg tttgttaatt gcgctgattg tgacgctgtc gatcacctcg gttgccggtt
     6241 cggcgcagca aatgattatt agcgccctgc acctgaacag cattgagtgg ctgaaaccga
     6301 cgtggcggtt gattggcctg gcgatttcca tcttcgccaa ctatctgctt ttcttctgga
     6361 tcttctggcg gttgccgcgc caccgtccgc gcaaaaaagc cctgattcgc ggaacatttc
     6421 tggctgctat tggttttgaa gtgattaaaa tcgtgatgac ctacaccttg ccatcgctga
     6481 tgaaatcccc ctctggcgca gcatttggtt ccgtgctggg gctgatggca tttttctact
     6541 tcttcgcccg tttgacgctg ttttgcgcgg cgtggattgc caccgccgaa tataaagacg
     6601 acccgcgaat gccagggaaa acgcagcctt aaaataggcc ggatgcggcg taaacgcctt
     6661 atccggcata cgattttatt cggcctggct ccccgtaggc cggataagat gcgccagcat
     6721 cgcatccggc tataatgcgc acataacctc ttgaaactct tccccagagc ctcttcagcc
     6781 atctatttgg gagcaaacaa tttcattcca actcataacc ccagcatata aatccagttg
     6841 gtaactttta tttaacctga aaccagtttt atccactatt tataaaatta tgtgaagcat
     6901 ttcatagaag aaaaatcact ggcctaaaca ttatcccctt tttgcctggt ttttgaccat
     6961 ttccgcgatt tgttacacat tgaaatatca cttttgctgt gcgtaatatg gctattcgtt
     7021 agccaaaaaa taagaaaaga ttatgcaagc aacagccaca acactcgacc acgagcaaga
     7081 atacacgccg atcaactcgc gtaataaagt ccttgtcgcc tctctcattg gcacggccat
     7141 tgagttcttc gacttttaca tttacgccac tgcggccgtt attgtgtttc cgcatatctt
     7201 cttcccgcag ggcgatccaa cggcagcaac gctacagtcg ctcgccacct tcgccatcgc
     7261 cttcgtcgcg cgccccattg gctctgccgt ttttggtcat tttggcgatc gcgttgggcg
     7321 taaagcgacg ctggtcgcct cgttgctaac gatggggatt tcgaccgtgg tgattggtct
     7381 gctgccgggc tatgccacga ttggtatttt cgccccgctg ctgctggcgc tggctcgatt
     7441 tggtcagggt ctgggcttag gcggtgaatg gggcggcgcg gcgctgctgg cgactgaaaa
     7501 cgccccaccg cgcaaacgtg cactgtatgg ctccttccca cagctgggcg caccgattgg
     7561 cttcttcttt gccaacggca ctttcttgct gctttcctgg ctactgaccg acgagcagtt
     7621 tatgagctgg ggctggcgtg tgccatttat cttctcggcg gtgctggtca ttatcggcct
     7681 gtatgttcgc gtgtcgctgc atgagtcgcc ggtgtttgag aaagtcgcta aagcgaaaaa
     7741 acaggtgaag atcccgctgg gtacgctgct gaccaaacat gttcgcgtaa ccgtactggg
     7801 tacgttcatt atgctggcaa cctatacgct gttttacatc atgacggtct actctatgac
     7861 cttcagtacc gccgccgcgc cagttgggct tggcctgccg cgtaacgaag tgttgtggat
     7921 gctgatgatg gcagttattg gttttggcgt gatggtgcca gtcgccggat tactggctga
     7981 tgcctttggt cgccgtaaaa gcatggtaat catcaccacg ctgatcatcc tgttcgcgct
     8041 gttcgccttt aacccgctgc tcggttctgg caacccgatt ctggtttttg ccttcctgct
     8101 gctggggtta agtctgatgg gactgacctt cgggccgatg ggcgcgctgt taccagagct
     8161 gttcccgaca gaagtgcgtt acaccggagc atcgttctct tacaacgtag cgtcgattct
     8221 cggggcttcc gttgcaccat atatcgcagc ctggttgcag gctaactacg ggttaggtgc
     8281 ggtggggtta tatctggcgg cgatggctgg cttgacgtta atcgccctgc tgctgaccca
     8341 tgagacgcga catcagtcgt tgtaagtatc gtgtgggcag ccaccctcac cccagccctc
     8401 tccctgaaag ggagagggag aaaagcggag cgagatgtta tatcgcgaac ataacagccc
     8461 tcccccagaa agtgaacgga aaagcggtac tcgttgtttc aacacgcaca aaacagtccc
     8521 ctcgcccctt tggggagagg gttagggtga ggggaacacg ctgctcccga actcaccagc
     8581 aaacacaatc caacctcatc acttcttcat ctgcgacaat atcgtccgac actgattagc
     8641 ctccccttcg gaaggtgaga tcagtgccag taacgccgcc gccggtgtta ccagcgttgc
     8701 cagtgccgca gcaacagcac cacgggcaat caacggcccg gctttcaccc cagcctgcgg
     8761 atttttaaac gtcccacgca catacagcgg cgaacgcagt gtgataatcc gaattccttt
     8821 actctccgga tcaatagtca aatccagctg ttcggaagca aaacttgccg tgccggtaac
     8881 gttaatcaac gcgttctcag tatcgaaagc aaagatctgc gggcgcgcca cgccgttggc
     8941 aatatccaga ttcgccgccg cgcagttcac ccgcacctca tcatcaccaa atatcgcacc
     9001 gacaatgtag ttgccgacat tcagcccaac aatctccatc aggttgcggc tcaccagccc
     9061 gtcattcatc aacagtttca ggttgccgtt actgttgcct aaaagtgccg ccaccgagtt
     9121 accgctaccg cgtagttccg cgtcaccgtt catttccccc agcgtcttct gcatcagttc
     9181 cacatcgggc atcagttctt tcagtttcag tcgacgagcc tgaatatctg cccgcccctg
     9241 catcggcttt ttatcgcctt ccagatgaat attcgccgca atgctgccgc ccgccatgcc
     9301 aaatttcagc ggttgcaggc gcaggtcagc atttttgagg atgatatgag tagaaagatc
     9361 actaatcggc aggctactgc catgctcaat gcgccgccct ttgaagcgaa catcggcatc
     9421 cataacgtcc catttatcgg tttcgaagcg gtcataaggc agcactttgc ccgcaggctg
     9481 aacgcttttt tcgcccttct tctgttcaga ccgtttcgac ttttctgccc ctttcccgga
     9541 gtcaacgcca attaacggtc ccaggtccgc cagccgcaat tgccgcgact cgacatcacc
     9601 ttccagtttt ggtcgtggct ttccggtggt gtagaccaga gaaccgtgga tatcgctatc
     9661 gccaattcgc ccattaaaac cgcgataatc aaagaccgac gatttttcag tgtcgatttt
     9721 cgctaccagc cgaccatccg tttcaaacgg cggggtatcg ggcagcagaa cgcccgtcag
     9781 ttcatagaga tcacccagtg aatcgccaga aaatttaagc cgtaaatcga caccgcccat
     9841 cttcattgga tcattcacga cgccatcaaa agcaacacgg gtgttaccag agcggaaatc
     9901 agcctgtacc ggaaacggcg tcccttcgcc acgcagcgcc agcataccgc ctatttttcc
     9961 cgtacccgtg agcggttcac cgttataacg tccctgcgcc ttcaggccaa aaacgtaatc
    10021 gcccaccttt tctttatccg ctttaccttt cgatccagta acttcgctga acggcagcgg
    10081 cttgcctaac ggatcaacaa aaatctccag atccgctttg cttactttgt catcaatggc
    10141 gatccgccct tgatcgaaaa gaatattatc cagccgaaac gaccatgccg acggctttgc
    10201 attcgcgtct ttgttatcat cgttggcgag attaaacgtc cagttattgt tcttttcgga
    10261 gaggcgaatc aggcgcgcgt cgggcttttc gagcttgatc cacggcagcc agaccgtttt
    10321 ggtcagcagc gccagcgggg ccagcgttgc ctctacgcgt ggcaaatgca ccatcgtgac
    10381 ttcgggaata tccggtggat tgccaagaat gatgtcttcc gcgtgtacat ggggccacgg
    10441 cacccagctg cgccagccag tttcttgttt ttgccgctcc cacaccacgc ccagatcgcc
    10501 acggatagcg aacggacgat tcaactccgc agagactttc tggttgatgg tcggtttgag
    10561 tcgattccag tcaaaagttg caatcaaaat gatcgccacg acaatcaaca acaagaaagc
    10621 cactgaaatc gcagcggtta ttttgcctgc cttgctcatc gttcgcttcc tcatattctt
    10681 cctgtgccag tcctaaagat agtccagcca ggcggaaaat gaggcagctt atagcgccag
    10741 aaccgccgta ttcagcgttt ctatcggtgc cgggcgtgaa agaaaccagc cttgtgcggc
    10801 gaatgcgggc gagttctgaa catcacgcca ctcttccggc gtttctacgc cctcgacaat
    10861 caccccgcga caatagcgat tcattagatg taaaagctga gaaaagagtg tgcgtccttc
    10921 cggcgactga cgcagcatca caaacagttc tcgcgcgatt ttgatgtagt cataacgcac
    10981 ttcacttagt gcagagaaat ttgccatccc ggtaccaaaa tcatccagcc acagcgggcc
    11041 aaattcacac atcgaggcaa aggttgaatc tttcggcagt cggatatgct ccaccagttc
    11101 gaaacgcagc cagggaagac gctcaatctg gcgcaggatt tttggttgct gacgcagggc
    11161 gatgagcgta gggccatcaa tattgaccga tgccagcagg ccgtgctcta taaagaagtc
    11221 ggctttttgc gccagcaaat caatctgctc tttcacaacc tccatccgat ggctgacggt
    11281 gatttcagta aaatagcgat ccggcggcag gcgttgcgaa gggttcaagg gatgcgtgac
    11341 caccgttaat agctccacgg ccattaaccg tccgcatgtt tgatagatcg gctgccaggt
    11401 gtaagcacgc tcacactgca accaaaaacg ccgttcctgc aagctctcga tgcttgcttc
    11461 agggttgctt attcgctgga taacctgcct tatcatcaaa gatgtcctgt gtttaaaagt
    11521 gatgctgccc ggactcgaca aagattatcg gcgcgcaagg gcagaacttt accgcctggc
    11581 cggggtaaat ttcctcactc cacaacggaa tctttcagga atacgttctg gctcaaaaaa
    11641 ataatggaac actgttttaa tatggttgac cagcaaacca ccacagcgca aactaacgct
    11701 aattttttac agatcaggtt cacgactatg tccaaaaaga ttgccgtgat tggcgaatgc
    11761 atgattgagc tttccgagaa aggcgcggac gttaagcgcg gtttcggcgg cgataccctg
    11821 aacacttccg tctatatcgc ccgtcaggtc gatcctgcgg cattaaccgt tcattacgta
    11881 acggcgctgg gaacggacag ttttagccag caaatgctgg acgcctggca cggcgagaac
    11941 gttgatactt ccctgaccca acggatggaa aaccgtctgc cgggccttta ctacattgaa
    12001 accgacagca ccggcgagcg tacgttctac tactggcgga acgaagccgc cgccaaattc
    12061 tggctggaga gtgagcagtc tgcggcgatt tgcgaagagc tggcgaattt cgattatctc
    12121 tacctgagcg ggattagcct ggcgatctta agcccgacca gccgcgaaaa gctgctttcc
    12181 ctgctgcgcg aatgccgcgc caacggcgga aaagtgattt tcgacaataa ctatcgtccg
    12241 cgcctgtggg ccagcaaaga agagacacag caggtgtacc aacaaatgct ggaatgcacg
    12301 gatatcgcct tcctgacgct ggacgatgaa gacgcgctgt ggggtcaaca gccggtggaa
    12361 gacgtcattg cgcgcaccca taacgcgggc gtgaaagaag tggtggtgaa acgcggggcg
    12421 gattcttgcc tggtgtccat tgctggcgaa gggttagtgg atgttccggc ggtgaaactg
    12481 ccgaaagaaa aagtgatcga taccaccgca gctggcgact ctttcagcgc cggttatctg
    12541 gcggtacgtt tgacgggcgg cagtgcggaa aacgcggcga aacgcgggca cctgaccgca
    12601 agtaccgtta ttcagtatcg cggcgcgatt atcccgcgtg aggcgatgcc agcgtaaacg
    12661 cagcatatgc aaaaggctgg ataaagtgtt acccctctat ccagctcatc cataaatacc
    12721 tgatggttgt gctaactcat caccatcagg cattactgtg caggtggaat atccgtcact
    12781 tcaggatgta cgtcatccgt ggcgacagag gtggcggcag cagcagtcga tggggccatg
    12841 atttgatccc aggccgcctg caacgctttc atgttaaatt ccggctcgcc tttcggctgc
    12901 agcagtatta acgccatatc attcgacaac tgctgacgca gatcctgatt taacatctcc
    12961 accgtcaggc tgttaaggaa atcctgccgc agtttctgat actgctccgg cgcgatatcg
    13021 acgacctgat tttgcaacga acgcatccgc tgacccatca gaatatcggt atcagcccgc
    13081 gcataggcgg caaacagttt ctgcagctcc agtttctttt gcgccactaa cgcattgaac
    13141 tcttcttccg gcagaccttt atcgcgaacc ttcgccagtt cacgcgccac cagattaagg
    13201 ttgctgttca gcttgtcgtt tggtgattcg atgttgatgg cacactgcgc acgcagatac
    13261 agcacacggc agtcaaatcc aagaccgatg tctttgctgt tactggcgct taacgcttgc
    13321 tgaacatgcc agaacagcgc ctcgcgggcc aggtccgcac gccagtagcg cagcagtgcg
    13381 gctgattcac gaatcggctg ccacggcgta tcccacatga tagataaccg gtcctgacgc
    13441 accgcgtcag tcataatact caccgcttcc gcacgcagcg gagaaagcgt cggcaccgga
    13501 gctggcgttt cacgtttgcc tttcagttcg ccaaacgttt tgtttatttg gtcgacaacc
    13561 gagcgcgcat ccacgtttcc caccaccagt agcgtcattg catccggggt gtaccatttc
    13621 tgatagaaat ctttaatttt ttccgcttca acgggttgtt tcagcggatc ggcaggatcg
    13681 tgacctaaca aggttgaccc tttcagacga tagcgccacc agccctcttt agtatcggca
    13741 ggccaggttg ccaccatgtc ctgactttgc agcgcgtggt tgatggtttc tggtgtgatg
    13801 gtcaatttgc cagtggcatt tgccagataa gagagcgctt ctttcagcaa atcgttacgg
    13861 ttattgggca aactcagatt aaacagcgtg gtgtcataag agacaattac cggcggcatc
    13921 gggcgtttag ggtcgatccc ctgctgccac aatgaacgcg cctgtgctgc gtcaaggcca
    13981 ccgctttgcg ttagcgcaat acgagggatg gcgtgactgt aaccgctctg ttgtgtactt
    14041 tcggcgagcg aaccggtatt aaccagcagg cgaatttcaa cacgatcgct gggacgctgg
    14101 ggggtggtca gcacttgcca ctgtaaaccg ttggaaagcg tcccctgttg ccatgctgga
    14161 tcaggctgga gcgcatctgc ctgcacatag ccagcagtgg ccatcatcag caaaccgccc
    14221 gctaaaagtc gaatttttgt gccctgcatg tgaacccctg atcaactatc ctggtaataa
    14281 aaaactgccc gttgtcggtc agtctaaatg acgttaaaaa cacttcgtgt tagaccgcaa
    14341 gaacatgaaa atgtcacgga agaagtgaaa taaacctgaa ctcacccagg tggtaagttc
    14401 aggcacaggg gtcaattatg cgcaaacacc cgcactcggg gaagggagtg cgggcataag
    14461 tgatgagatt aagaggataa ttcgtgcgtt ttgccatccg gcgcacgatt attcagcaca
    14521 tcgtccagtt ttttgtggtc cagttctttc acccacttag caacgacaat ggtcgctacg
    14581 ccgttaccga ccaggttagt cagcgcacga gcttctgaca taaagcggtc gataccgagg
    14641 atcagcgcca gacccgctac cggcaaatgg cccaccgcag agagcgtcgc cgccagcacg
    14701 ataaagccac tacccgttac ccctgccgcc cctttagaag aaagcagcaa cacgattaac
    14761 agcgtgattt ggtggacgat atccatctga ctgttagtgg cctgggcgat aaacaccgcc
    14821 gccattgtca ggtatatcga tgtgccatca aggttaaacg agtagcctgt cgggatgacc
    14881 agccccacca ccgatttgcg gcagccgagt ttctccatct tgtcgagcat acgcggcagc
    14941 gccgactcgg aagatgaagt ccccagtaca atcagcagtt cttcacggat gtagcggata
    15001 aatttgaaga tactgaaacc agtcgcttta gcgattgaac ccaataccag caccacaaac
    15061 aggatacagg taatgtagaa acagataatc agctgcccca gttgcaccag tgtgccgacg
    15121 ccgtatttac cgatggtaaa cgccattgcc ccgaacgcac caataggcgc cagacgcatg
    15181 atcatattga tgatgccgaa gatgacctgc gagaaacttt cgatgacgtt aaaaatcagt
    15241 tggcctttgc tgcccagacg gtggagcgca aaaccaaaca gtacggcaaa cagcagcacc
    15301 tgcagaatgt taccgctggc aaatgcgcca atgacgctcg ccgggatgac atccataatg
    15361 aaggcgacaa tgccctggtc tttcgcctga tcggcgtaaa ccgctaccgc tttcgcatca
    15421 agcgttgccg gatcgacgtt cattccggca ccaggctgca cgacgttaac gatgataaga
    15481 ccaataatca gcgcgatggt actgacaatt tcaaagtaaa gcagtgcgac tgcgccggta
    15541 cgaccgaccg ccttcatgct ttccatgccc gcaatgcccg ttacgacggt acaaaagatg
    15601 acaggagcga tgatcatctt aatgagctta acgaagccgt cgccaagcgg tttcatttgc
    15661 tcgcctattt caggatagaa atggccaagg agaataccaa tggctatcgc tgtcaggacc
    15721 tgaaagtaaa ggcttttaaa cagagaggtt ttcatagggt gtcctttagt aaaaccacag
    15781 gtcttgtaag gttatggggt acctgcggcc ttaaaataac acccagacaa catcacagaa
    15841 atgtacctgg atcataattg aaacaaaaag gttaaaaagt ttgagctggc tcgcacaaac
    15901 cagcactttt taaagttttg taatcagttt ggggtagcta cttttcttcc aggtaactct
    15961 cttcgaagat ttcaataggg agtgggcgag caaaaaggaa gccctgggca ataccaacgc
    16021 ccgcttttgc cagccagtcg cgttgtgctt cagtctccac gccttcggca atcatttgta
    16081 agttcaggct ctgcgccagc atgataattg cagcaatcat gctgctatct tccggcaagc
    16141 cttcaacaaa cattttgtcg attttcagta cgtcgattgg caacgatttc atatgctgca
    16201 gctgacgcag ccctgcgtag cccatgccga aatcatccag cgccacccga actccggcat
    16261 tgcgcagcgg acggaggatt gccaccgcag catgagggtc gtcaatacgt cggctttctg
    16321 tcacttccag aatcagtgtt cccggctgaa tgcgatagcg ggttaacagt tccagcatat
    16381 ccgccaccat atttgggtgc atcagttgca gcgcagagag gtttaccgac aagggcagca
    16441 taatgccgcg ctcttgccag gctgcaagca atcgacagga ctcttccagc acccagtgac
    16501 cgacggtaac catcagccca cagcactcaa tgcgatcgat taagccatcc ggcaggtccc
    16561 aactgccatc cggttgctgg atacgcagta acacttccgc actgaccagt tttccgctgg
    16621 tcatctcgac ctgtggctgt aaccaaatag caaactgatg attttccagt gcattaagga
    16681 tatcgctctc ttccgtcaac cgcttctggg cggcttccat ctgctgcgga tcaaagaact
    16741 gaatctgatt cttgccttta tggcgagcgg taaatgccgc agaaatagcg cgactgtaaa
    16801 gctgttcggc ggtgagatcg ccgtagaaca tcgccacgcc aatgctacag tgcggacgga
    16861 gttgaatacg ttcaatcggc aggcgctcgc tcatgatagt gagcacttgc tgacctaagg
    16921 tgattgcgtg ccacggttcc tgtacaccgt tggcaatgac agcaaagtca taaccgctaa
    16981 tctgcgcgag gatcatacgt ggcgacagta ccgatttgag tttttccacc agcgtcagca
    17041 gcagaatttc tcgttgcgcc tctttcagca cgcccgcagt atcacgcagg gtttcacagg
    17101 tgatgatcat cagcgcggtg gtttgtttac gcgcgacaac ctgctccagc atctccatca
    17161 gcaaggcttt gttcggcaaa tccgacaccg ggaagcgcat cgcattttcg ttctgttctt
    17221 cataatggcg ctgcagcaat tgctggttga ggttgtaact gcgcaccaac ataccgattt
    17281 catcgtcctg atgcagacgc ggtaatgcca gttggtgacc aacaagctcc tgggctggga
    17341 tggcgttaag ttcgcgagca atattgcgta acggatgcaa aatcaggcgg ttaatgcacc
    17401 agctgatggc gacggtcagg ataagcgaca aaagtaagta aatggtcact aacattgaga
    17461 gggtgctcat cacgaactta tacatacgga aggaatccgc ctgtagtacc agataggcaa
    17521 ttggctgcgg gtttgccgga cgttcgagcg agtaaacgcc cagcgagatt tgaaccggta
    17581 gctcaaacag gcgagtaacc attaccggca ccgggcgctc tggaataaaa cttttacgca
    17641 gcgcctggaa ctggttaggc agcactacat cggcacggct gaccacgcct gccggtttaa
    17701 tgctggcaag aatagcttcc gcttcgggaa tatcgccttt caaaatagct gaagataagg
    17761 gttcgcggac agagcgagca atgctttcca gttgcgtagc cgtgttatag cgattctgtt
    17821 ggaccagatg gaacagcaaa acggtgcaaa aaataaaaac gaacaccagg acaacggctg
    17881 ccaccattgc catctgcttg attgttaacg agcgacttac gcgcaaattg tctccccccg
    17941 gaaatcccag attgccgccc gagtataccc gatcgcagcg gcattaagag aggcgctatc
    18001 tgaaaactta ccagtcggcg taaggtatca gcggctgcgg cggtaaatcc atgtcaccct
    18061 gccatccggc ggcggaataa cgtacgtaga gcaggaaatg gctgggtgcg taatctttcg
    18121 cctgctggat atcaattgcc gtgccaacaa accagttgga agtaacacgt cgttcaagta
    18181 atgcccgcgc cgtgtagccg aagccctgac tgctgccgcc atcgttggat tgccgcgcag
    18241 cttcttcctg ccagtcggtc gggatcagat tcatcagcgg ataacgcggc atggttttgg
    18301 tgcgtgaatg cgaccacgag ccagacgccc ccagctccca cgaccagttt tccgtgcgct
    18361 cccgccacat caccggtatg gcaaacgaca ggtattcctg cggactgtag taaccgccct
    18421 gaccgagtga gtagccactc agatctttgt cgtaatgcca gatcatgttg ttcaggccga
    18481 ttgtgacgcg gcgattgttc tggttaatga ccttatagta atagcccgtc atccagcgca
    18541 cgcgccagtt atcttcgaca tttttaccgg ttaactggtc gccactaagc gatgcccaga
    18601 cgccgtttgc ttcaccttta tcgtagctca gacttagccc cacaccgtcg gcacgtacgc
    18661 caccccattt tttcccggta ttgctcgggg agtctttttg cccaccaaag gccagcaaag
    18721 aactggagat gggccgacgg tgggcgttaa cggtgtaacc cagcggcccg atatcatcgc
    18781 tgtaactgat gccgccgacc acatccacca cgttgaagcc catcggcgtg gtaccgatat
    18841 cccagctcca gacgtcattt cgccagccga ccgccacgct ggcaccggaa tccgactggc
    18901 tgcggttgcc gctacagtcc tgtaatgtac aggtgcccca gttgtcatcc catttgccat
    18961 cggcattagt ggagaaactg ccgacgttca tattgacgaa atcactgcga aagaacatcc
    19021 gcccgtcaga atacggcgca tccacctgca acatggtagt gtgcgctttc agatcggagt
    19081 aaccaccggt gccgctcgaa ccccagtaat cgtgctcgag ggtgacgtta agatcctgct
    19141 ggcgatagag gtccgccgca tcgctgcgca cgccgcgttt cagccagtca tctttctcgt
    19201 cgttacgggt cagtcgggtg aatgtgtcgt tatcctgcgg acgcgtcgta gtcacaccgg
    19261 atgcgaccat ggcgtctttg taggtttcca gcgcctgctt tggatcgccc gcctgcgctt
    19321 caaacttcgc accgtcgcgc agcaccatcg cgctttccat cgacggtggc tgagattttg
    19381 cctgcgggat caacttatta aacgtctgct gcgccgctgc ggtatctcca agctgcgcct
    19441 gcgccagcgc cacgcgccgc tgtgtgttca gcgaggcgtt atcggtagcg ggcagtttcg
    19501 ccagctggct acgtgccgcc gctgtgtcac cggcagcaat atccacttcc gtcagcccca
    19561 gaatggcatc ggcgttagtt ggctccagcg tcaggacatt ctgatatgca gcgcgggcgg
    19621 cggtgtaatc acgtcgctgc tgcgcccagt cagccagcgt gaggtcaata cgcgtggaag
    19681 gtggttgctg acgcagcatc gcttccgctt cagcttcttt gccgctttct cgcaggcgat
    19741 tagcggtttc cagcacctga tcgctttgca gtcgattaac cagctcctga atattgctgt
    19801 tccactgcgc gcgcggcagg ctgttgatat gcgccagcgc cgctctgtcc tggtcatgac
    19861 cagagaggta cagcccgtaa gcgtaaacct gctccgggtc gttcggcttc tgctgcgcca
    19921 gattgcgcat taacgtatcg gcctggctgc gttgtccggc ctgccagaga tcctgcgaaa
    19981 gtcggtaagt aatccatacg ctgccggggt ccagcgccag tcgttgccgc tgaagtgctg
    20041 ccgcctgcgc ccatttgccc tggttttcca gtgcctctgc ctgctgtgcc agacggtcgt
    20101 tttgcaggct gcgttcgata tcatcaatgc tacgccgctg actggcagag agcgaggcga
    20161 taaacgcttc agctttttct ggcgattgct ggcggtaaat atttgccagc ccgcgcacgg
    20221 cgttagtgtt gccgctgtcc atacgcaagg tctgctgata ataacgttcg gcggcgggat
    20281 aatcttttcg cgccatcgcc acatcgccca gccccagcac tgcataactg tcggtgttat
    20341 cgacattacg cgcctgctgg aacaggcgtt ctgcccggtc aggattattg gctttcagcg
    20401 cagcatcgcc ctgctggatc gccagccagt agcggtttac tttcagcaga ctgttccatt
    20461 tgtcgttgtt gctgctgtgc gggtccagtg cgagggcttt ttccagattc gccactgcat
    20521 tggcgcgatc gcctttctga gaatacgcct ggcccagcgc ccccagagct tcactgtctt
    20581 tcgggttcgc ccgcaccgcc tgttgtagtt cgggaatggc tttacccgcc ataccagagt
    20641 ccaccgccgc taaaccttgc gcacgagcgc ggaaagcagg atcggccagc tgtttttgct
    20701 gttctgccag ttgcgattgc gcagccgcca cgctatcgcc atcactaaag atcgagagat
    20761 attttttcag cgccgacaca ctggcatcac tgacgggcat gtctttaatc tgcccgtacc
    20821 agattttaga ggccccttcg cgcccggcgt tcgattttgc catctgttcc aggacggcaa
    20881 aaccttcgtc acggcgatcg ctactaaaca gcaatagcgc cagattgttt tgcaggcccg
    20941 tattgcccgg tgcatccgca ttgatgcgtt ttaactgatt aatcgcttcg ccacggcgag
    21001 ccggaatttt cgccaccgta ctccagtact cgacagcaat gtcaccttcc ggcggcgcac
    21061 cgttgaacag tttgttgtaa ctcgccacag cttcttctgc atgaccggtc gtcgcctgca
    21121 atcgtgcctg ttgcagtgcc tgacgaccat ccggcgtgga aagtagcatc gtagtccgcg
    21181 acgatttata cgcatttgaa ctcggcgcta actgcgacag ccgatcgagc tgtttttgcg
    21241 cgccatcaat atcgccctga cgtaacaaag aacggaaacg ggcggcaacg acgtccgggt
    21301 tattcggatc aataagttcc agccgatata acgactgttg caccagatct tcacgatggg
    21361 tcgcttcgcc taaccgaact tgctccagca actgttgctg agcggttggt gctgcctcga
    21421 ccatcggcat gacggccaga ccgagggaaa gcgtgaatat gtttagtgtg aatttgcgca
    21481 ttcctggccc cagtcaggta ataactcacc ttttgtcgag aagcggaaac ggtgttgatc
    21541 ccagccttgt ccaaacaggg tcagcacata gttgtaatag gcatcgctgc cgggaaagtt
    21601 atcggccacg cgctggcgct gaacggcctg cgcatcgcgg ttttgtaaaa agggcagcat
    21661 ggcggcagaa aaaccgaccg gtcctttacc ctgcgctttc cccgtagcca catccacttt
    21721 ttccggcgga taaccgtttt tctcagtgaa tgtcgccatc ggtttaaacc ggttgagcat
    21781 ccgcgctttt tgcggatcgc tgtcaggcat catgcctacc cacatgtaaa cgcggatagc
    21841 gtcgtagctg ctgatcaatg ttttttcggc ttttagctgc cagcctttgt ctttctcata
    21901 gcgcacccag tctggcgaaa aacctttcgg ggcggtttcc agcaataaac gttgattggt
    21961 ttcgcgcagc gtggtccacg gcgcgccaaa gcgggtgaaa tactgcgcca gcgtcggcgg
    22021 caggtagctg gggttaaaac gccagctgtt atcctcagca aaacccactt tgcccggtaa
    22081 caacatggaa cccagcccag gcaccgtcac cacttcctcc cgcgcgatac gttttagcaa
    22141 cgcgctgccg atgtcggtat aacgctgctc tttccacaaa cgccccgcct ccagcaacga
    22201 ccaggccatc cagatatcac catcggaggc cgaattgctg tccagcactt cccacttact
    22261 gttctctttc ttgccccaca gccaggcggg caaacgttct tttaaagaac cctgagcgag
    22321 attgttctgc gtccagtcga gaatattatc gaaagctgca cggtcgttag ccgccagggc
    22381 aaagaacatg ccgtaacttt gcccttcgga ggtggtgatt ttgcgcgcgt cgctggggtc
    22441 gatgacgcgc ccttcctgac tgatgtaatc ctttttaaac tgctcccagg caggccaggt
    22501 acaggctgcc tgaacactaa aggcagccag cagcagcatc gtcacgattc cactacgcaa
    22561 cacattcatc ttcaattact cgttatccgg gttaagacga cgacgactaa taattcgcag
    22621 cagacgccac agtacccatg ccagcaatat cacactgata gccgccagca ccgccagcag
    22681 aatcggatgg tttgccagcg cataccacac gcgctcgaac cacggcagat gacctacgta
    22741 ataaacgtcg ccaacacgta ggctgttgat accggactcg cggatcaccg cgaccgaacc
    22801 gaacatggtg gcgcgtttgc cgctatcgtt caccgcatcg ttaagcattt cataaccgcg
    22861 tgggctatct gccaacagcg caatcacgct gcgctggtcg ttatacggcg actggaagcc
    22921 aatcaccgcc gccatcgcac cggaagaggt cagcgttgac cgggtttctg ccgcgcgatc
    22981 gctctcgtcc ggcacaatgc cggggaacgg ggtctggcgc atcggtgttt tcacccagct
    23041 ttcggtcgcc tgcaccaata ggtcgatctg cttatcgtct ttcagtttgt ccgggatacc
    23101 accgatgatc atgatgtcgg catctttgcc ctgaatggtg ctgccatcat cggtcaccgt
    23161 cagattaatc gccgggaagc ccgtctgtgc gccgataaaa ccaacagtat tcagcaacgt
    23221 ttccatctgt gcttcgttag gcgctttcgg catcacggtg atggtttgcg acagatccgc
    23281 catccggctg aatgggaagc ccgcgttagc aaaggcgcgt agatccggca tcgggatgaa
    23341 gtggtaatac ttcgagaagt cgatggtgga gtcgtcacca atcaccacat gattctgcac
    23401 cggctggaag gtaatacagt tatccaccga accgcccggc atcgggttca tatactcaaa
    23461 gtcgaagcgc agctggttgg tcgcgcccag tttcagcgcc ggaatagaga catctgtttt
    23521 gccatccagc aaaccttgta ataccggaat ccgcagcagc aggcggttcg cctcctgttt
    23581 gctgctcaga ttgaaggatt gcaggaactg gttattcagg ctgatatcca tccgcgaact
    23641 gtctttcacc ggcggcatgg tgtagcggta attaatatcc atatcaatgc cggtactgcg
    23701 catcaggtag agatccggcg gcaggtttag cgaaacgtta atcgctgctg gctcaagacc
    23761 gctggattgt aactgttctt cataggtttt cagttcgcca aatgtgaccg gacgatcggt
    23821 acgtacccag ttcggcgcat cgtacggctt acgcggtagc agcggtttca cttcattcac
    23881 taccacgctt tcaccacgga acagaatgtt accctgagcg atacctttcg ctgcctgcaa
    23941 caggtctttg tcgtcacgac caaacaccac cagcagtttg acgtaaggat tctgcggatg
    24001 gttaatcatc tcaatcacag gggcttttac cgccggatga tcgcgcagga agtccggccg
    24061 tttgtcgttg gttgcaaaga caatggcatt acgatccggc agttggttat agagcaccgg
    24121 gaagttctgc ccacgccagc cagaacgcga accaaaccac gaggcgacaa tggcagaggc
    24181 ttgttgcagc ccaacatccg gcgcacccgc aaagaccatc ggcaaggtgt tagtacggtt
    24241 atcgcgcggg tcaaagaatg gcaccgggaa gtgtgacagg tcattcttca cattcagggt
    24301 ctgataggtc agatccagtc cactgctccg cccaacatcc agccaaagcg tggtgctggc
    24361 cgggttttcg cacacgtcct gataatggcc gacaaactcc agccgcacac ggttgaagtc
    24421 ggtaataaac agtgggttaa tgggcatttg cgccagcgtt tttttaccca actgttcttt
    24481 ggtcactggc agcacgccca tcagctcatc attgagataa acctttaact gcgactggac
    24541 aggcagtaac gatggcgatg gcgtgtattc gaggttgagc atcgccttcg tcaccacttc
    24601 atcgctgcgc atgccaaact caatgctgcc gttcgggtta atgccacgta gcaccatgct
    24661 gcccggcggc ggcgcaattt gcgcaaaggt cagcttcaca tcacgcgaag gaccgttctg
    24721 cgccacgact ggcgcatcag cgccctgcac gcccggcatc acttgcccca cctgcggatt
    24781 ttgttccgtc tgggcggcta cagctggctc agcattgatc agtggttgcg ttgctggcgt
    24841 cgcctgcgtc atgaaagagg ggaacgcact catccccata gccactgcac aaatccagaa
    24901 tagttttctt ttcatcgcgt tatcatcatt gttgagccaa agcctgatcc gatggttgtg
    24961 ccgtttcgct ccgctccggg cggcgcggaa taaacgatac aacccaggaa accagagaag
    25021 tcagcacacg gaatatgccc ttcaccgaag aaggcgcaaa ctccgccaga tggcggtagc
    25081 cacggaagcc gagcttcaga atatccagca gactttccag cggcttatct tccgggtagc
    25141 tgtcctgcca gagcgcccat gtatccgcac gggcaaacgt acactgcaca aaatcgatat
    25201 gttgctgggt ggtgagcggc attaatttca gcccaacttc attacccatc acgcgcgcca
    25261 cctgggtcgg gaagacgtat tcctgctgac cgcgtttaag caacagattc actttctgcc
    25321 cttccagaat ctgcgcctga ccgttgatct tgatccccaa accaccgtcg gagaaatcct
    25381 gaacggtaca cgagaagagg tgaccatctt cgcgggcaat tgccgcgggc atcgtcatct
    25441 ccacgcggtg cgatcggcgt acctgtttgc tttctaccga tactgcaact gcgccgccaa
    25501 gaacaatcag gttgtagaat acccacacca tactgacgac cacggtgagc atctcggttg
    25561 gcgggccata gaagtagcgc cagatgccta ccgcaacgcc caccaggttg agcaggacaa
    25621 ggaagatgta gggccgcgag atcacccagt cgacgtactc ttcttccacc agtccacctt
    25681 tggcggtgac gttaaatttg cctttgtgcg ggttaatcag cgccaccagc gtcggtggtg
    25741 cgatatacca cgccagcacc gtttcgtaga tttcactcca gaaagagtgg cgatatttgc
    25801 cctggatctt ggagttggtc aggctggcat ggatcatatg cggcagcacg aacagggcga
    25861 tcatcaacgc tggcgcatag atgatgtagg catgaagcag caggaacgcc agcggcgcag
    25921 tcaggaagat cagtcgtgga atgcccgaca agaagtggaa catggcgttg acgtagcaca
    25981 gccgctgggc gaatttcagc cctttaccgg taagcgggtt atcgagacgg aagatttgca
    26041 ccatcccgcg tgcccagcga atacgctgac cgatatgcgc cgacagactt tcggtcgcca
    26101 gccccgccgc ctgcggaata cgcatatatg cagaggtgta gccacgacgg tgcaggcgca
    26161 gagacgtgtg cgcatcttcg gttaccgttt cgacagcaat accgccaatt tcatccaatg
    26221 gtttgcgacg aattactgca caggaaccgc agaagaaagt ggcgtcccac atatcgttgc
    26281 catcctgcac cagaccatag aacagcgtgc cttcgttcgg cgttttacga aaacgcccca
    26341 ggttgcgttc aaacgggtcc ggcgagaaga agtggtgcgg cgtctgcatc atcgccagct
    26401 gtttctcttt caggaaccag cccatggtca tttgcaggaa cgatcgcgtt ggtacgtggt
    26461 cgcagtcgaa aatcgacacg aactcgcctt tggcatattt cagcgcattg ttgatgttgc
    26521 ccgctttcgc atgttcatga gtggtacggg cgatatattt cacccccacg ttttgcgcaa
    26581 actggcgaaa ctcttcccta ccgccgtcat cgaggatcca gatgttcagc ttgtctttcg
    26641 gccagtcgat acccagcgag gcgtaaatag tatttttcac cacgttgaga tcttcgttgt
    26701 aagtcgggac aaagatatcc accgacggcc acagcgacat atctttcggc aatggcaccg
    26761 gctgacgatt cagcggccat actacctgga agtagccgag caccagcaca atccacgcgt
    26821 acgtttcagc gaagagcaga ataagcccgc acaccaggct gaccggatcg tcccagttca
    26881 gcgtagaggt gtaacgccac cagatataac ggcaagaaac ggtcagcgac agcacaatca
    26941 acattagcgc cgagaagcgc cccggcatcc gccgtacgat cagcgctacc ccccacagca
    27001 gcatcaggaa gataaactgc gccagcgggt taaacggctg agtaacgcag attaacgcca
    27061 gaatcagcga gaaggtgacg atgataccga ggatcaaccg ccgcgccccg gcactcaaat
    27121 gaccgagctc ttttttctca tccagatgct gtgttttatg gctaacgcgc tcaggcagct
    27181 cgttcatcca ttgatggtaa cgtccacgaa tattttgcag acctgaaaat gcccgcctgc
    27241 gcggtttcgg cgtttctttg cgcgatgcac cgatcaataa ccagcatgtt tgaatgagat
    27301 aacggaccgg gtccagcgga cgcggacgcg aggcgttgat atgcggatac aggtttttat
    27361 gttctgcgcg aatacgctgc cagcgcgggt gctccagcgg aataaaaatc caggccagga
    27421 tcatccagaa acagccgagc gtcgcgctga aagccgacgc accgtggcga cgataatcgc
    27481 gataacgccc gataagccgc gcgttgaccg gcgggataag caaccaccgg gtcaggatac
    27541 tcatgatgca ctcccgactg gcgttttcag cccggaatag ttcaacaggc accagttcgc
    27601 cagcgtcagt atctcttctg ccgccagcgc atcactgcgg tattcaccga ctggttgctt
    27661 agccgccagg cattcagcca tcgcttcatc acgatgaatg agcatcggca gcaatcggcg
    27721 ctggctttgc aaccaaagct ggtaaatatc gtcctgaacc tgactgccaa tacggaagtt
    27781 attaatcaaa atatgtgcgc catccggcag cgcttgctga tgcaggcgga tatggcagtt
    27841 ggcatccaca ttgacgatag ccagcgagtg atcgcacaaa ctcagcagct ggtgggttat
    27901 ctgcgaggca tcacgcggta agtcgattaa aatccactgg taacgcccgc tggcttttag
    27961 ttgctgtaag ccggagcaaa tatcgctcag ccgggtttgc cagtgctgtg gattttcttg
    28021 ttcttcaatg gataactgac caaaaggcag caaatcgagc tgcgaggtgt agcgcaaccc
    28081 agcgtcacgc cagtcctggc catccagcat cgctctggcc cagccctgac ggtgggtaaa
    28141 atcaacatta aatgacaggc gcaacaagtt gtccgggcag gcatcgacca ccagaacatt
    28201 ttctcccaac atttgtaatg accaggctaa tgcggcggtg atggttgttg tccccacgcc
    28261 tccccgcacc ccctgcaatc ccagtacggc cattcaccaa tcccctactt ttgttgcgca
    28321 aactctgcca gcaacggcca gcgttttaat gccgcggcca actgttcgcg ttgggaaata
    28381 tcggcataat caatatcagg cagtgaaaat gcctgcttta acgccacaat atcattctgg
    28441 aagatatagc ctatcgcggg atcaggcaga gtatctggtt cattgttatt cattttgctt
    28501 gatccctatg aaataaccgt accgttcatg agacaattct aaccgtgagc atttcgccgg
    28561 tttattgttt acggcgagac gggaatttgc tcactttttg actttccgtt acgcaacaga
    28621 aaaagttaaa aacgaaaaga ctaaaatcac acgtcttgtg accttctaaa ttgattcaca
    28681 tgctaaatct gataagtttt aatttcaatg gtaggtttat ttcttagctt tcgctagtaa
    28741 actgataatc agacaaaatg gtgacatgag ggacattgtg gaccctgtat tctctatcgg
    28801 tatctcatca ttatgggatg agctgcgaca tatgccagca ggcggcgtct ggtggtttaa
    28861 cgtcgatcgc catgaagatg ctatcagtct ggcgaatcaa acaattgcat cccaggctga
    28921 aaccgcacac gtcgcggtca ttagcatgga cagcgatccg gcgaaaatct ttcaattaga
    28981 tgattctcaa gggccggaaa aaataaaatt attttcaatg ctaaatcatg aaaaaggtct
    29041 atactatttg acccgtgatt tgcagtgttc tattgatccc cataattacc tttttattct
    29101 tgtttgcgca aataatgcat ggcaaaacat tcctgccgag cggcttcgct catggttgga
    29161 taaaatgaat aaatggagca ggttaaacca ttgttcgctt ttggtaatta atcccggaaa
    29221 taataacgat aaacaatttt cattgttgct tgaggaatac cgttcacttt ttggtcttgc
    29281 cagtttgcgt tttcagggtg accaacattt gctggatatt gccttctggt gcaacgaaaa
    29341 aggggtcagc gcccgtcagc agcttagcgt tcagcaacaa aatggtatct ggacattagt
    29401 tcaaagcgaa gaggcggaga tccaaccacg cagcgacgaa aaacgcattc tgagtaatgt
    29461 tgctgtactg gaaggtgcgc cgccgctatc ggaacactgg caactgttca acaataacga
    29521 agtcctgttc aatgaagccc gtaccgctca ggcggcgacg gtggtctttt ctttacagca
    29581 aaatgcgcaa atcgagccac tggcccgcag cattcatacc ctgcgtcgcc agcgcggtag
    29641 tgcgatgaaa atcctcgtgc gggaaaatac cgctagcctg cgcgccaccg atgaacgttt
    29701 gttattggcc tgcggtgcaa atatggttat tccgtggaat gcgccactct cccgttgtct
    29761 gacgatgatc gaaagcgtgc aagggcagaa gtttagtcgc tatgtgccgg aagatatcac
    29821 taccttgctg tcaatgaccc agccgctcaa actgcgtggt ttccagaagt gggatgtgtt
    29881 ctgtaatgcc gtcaacaaca tgatgaataa ccctctatta cctgcccacg gtaaaggcgt
    29941 tctggttgcc ctacgtccgg taccgggtat ccgcgttgaa caagccctga cgctgtgtcg
    30001 ccctaaccgt accggcgata tcatgaccat tggcggtaat cggctggtgc tgtttctctc
    30061 attctgtcgg attaacgatc tggataccgc gttgaatcat attttcccat tgcctactgg
    30121 cgacattttc tcaaaccgta tggtctggtt tgaagatgat caaatcagtg ccgagctagt
    30181 gcagatgcgc ttgcttgccc cagaacaatg gggcatgccg ctgcctttaa cgcaaagttc
    30241 taaaccggtc atcaatgccg agcacgatgg tcgccactgg cgacgaatac cagaacccat
    30301 gcgactgtta gatgatgctg tggagcgctc atcatgatga ccatcagcga tatcattgaa
    30361 attattgtcg tttgcgcact gatatttttc ccgctgggct atctggcgcg gcactctttg
    30421 cgacgcattc gcgacacctt acgtttgttc tttgctaaac ctcgttatgt taaaccggcc
    30481 gggacgttac gccgcacgga aaaagccagg gcaaccaaaa aatgactcaa tttacgcaaa
    30541 ataccgccat gccttcttcc ctctggcaat actggcgcgg cctttccggc tggaacttct
    30601 attttctggt taagttcggc ctgttgtggg cgggatatct taacttccat ccgctcctca
    30661 atttggtgtt tgccgcgttt ctgctgatgc ccattccgcg ctacagcctg catcgcttgc
    30721 gccactggat tgccctgccg atcggctttg ctttgttctg gcatgacacc tggttgcctg
    30781 gcccggaaag cataatgagc cagggttcgc aggtggcggg gttcagtacc gattatttaa
    30841 tcgaccttgt cacacgcttt attaactggc agatgattgg ggccattttt gttttattag
    30901 tggcctggtt attcctgtca caatggattc gcattaccgt ttttgtggtt gccatactgc
    30961 tatggctgaa cgtacttacc ctggcgggac caagtttctc cttgtggcca gccggacaac
    31021 cgacgaccac tgtaacaacg acgggtggta acgcagcggc aaccgttgcg gcgacgggtg
    31081 gcgcaccggt agtgagtgat atgcccgcac aaactgcacc gccaacaacg gcgaacctta
    31141 acgcctggct gaataatttc tataacgcgg aggcaaaacg taaatcgacc ttcccgtctt
    31201 cgctgcccgc tgatgctcag ccatttgaac tactggtgat taacatctgt tcgctttcct
    31261 ggtcggatat agaagccgcc gggttgatgt cgcatccact gtggtcgcat ttcgatattg
    31321 agttcaagaa ctttaactcc gccacctcct acagtggccc ggcggcgatc cgtttactgc
    31381 gcgccagctg cgggcagact tcgcacacta atctgtatca accggcaaat aacgactgct
    31441 atctgtttga taacctttcg aaactgggct ttacccagca cctgatgatg gggcataacg
    31501 gccagttcgg cggttttttg aaagaagttc gcgaaaatgg cggcatgcag actgaattga
    31561 tggatcaaac aaatctgccg gttattttgc tgggctttga tggttcgccg gtttatgacg
    31621 ataccgccgt gcttaaccgc tggctggacg ttaccgaaaa agataaaaat agccgtagtg
    31681 ccacgttcta caacacgctt ccactgcatg acggcaacca ttatccgggg gtcagcaaaa
    31741 cagcggatta caaagcgcgg gcgcagaaat tctttgatga actggacgcc ttctttactg
    31801 aactggagaa atcgggtcgt aaagtgatgg tggtcgtggt gccggaacac ggcggcgcgc
    31861 tgaagggcga cagaatgcag gtatctggcc tacgtgatat ccctagcccg tctatcacag
    31921 acgtccccgt tggggtgaaa ttcttcggca tgaaggcacc acatcagggg gcaccgattg
    31981 tcattgacca accgagcagc ttcctggcta tctccgatct ggtggttcgc gttcttgatg
    32041 gcaagatttt caccgaagac aatgttgact ggaaaaaact caccagtggg ttgccacaaa
    32101 cagcaccggt ctccgagaac tcaaatgcag tagttattca ataccaggat aaaccgtacg
    32161 ttcgcctgaa cggcggcgac tgggtgcctt acccgcagta agcacgaaaa aggccgcaga
    32221 gtttaacctc tgcggcctga tccgggcgca aattgccatt acggcagcct gacgcccgca
    32281 tgacacgtta cttccgctta ttcagccagt tcacgatcat actggcaaga atgccagcaa
    32341 tgaccggagc cgctaaatca tgccagaagg ccatgcccag ctctgcgagc gtcatatagc
    32401 cgcctgtgtt gtaatgacaa cgtttcgcgg ctattcttga gttgtttggg ttcaagatta
    32461 gcccccgtta tgttgtcagg tgcatacctg caacgtgcgg gggttttctc tctccagcaa
    32521 ccaatgccac cagggataaa gcccccgcaa cattgcgcct caccggaacc tttccggctt
    32581 gccgctgatt ctacgactgt ttttctgttc cgaaatccat cacttactca ctctgtgctg
    32641 cgcattccgc agaatattat ccgcttgcaa ccgttgcatt tttcttacga aaaaggccgc
    32701 agagtttcac ccctgcggcc tggtccgggc gcaaattgcc attacggcag cctgacgccc
    32761 gcatgacacg ttacttccgc ttgttcagcc agttcacgat catactggca agaatgccag
    32821 caatgaccgg agccgctaaa tcatgccaga aggccatgcc cagttctgcg agcgtcatat
    32881 agccgcctgt gttgtaatga caacgtttcg cggctattct tgagttgttc aggttcaaga
    32941 atggcccccg tgatgttgtc aggtgcatac ctgcaacgtg cgggggtttt ctctctccag
    33001 caaccaatgc caccagggat aaagcccccg caacattgcg cctcaccgga acccttccgg
    33061 cttgacgctg attctacgac tgtttttctg ttccggaatc cgtcacgtct tcactctgtg
    33121 ctgcgcattc cgcagaatat tatccgcctg caaccgttgc atttttctta cgaaaaaggc
    33181 cgcagagttt cacccctgcg gcctgatccg ggcgcaaatt gccattacgg cagcctgacg
    33241 cccgcatggc acgttacttc cgcttgttca gccagttcac gatcatactg gcaagaatac
    33301 cagcaatgac cggagccgct aaatcatgcc agaaggccat gcccagttct gcgagcgtca
    33361 tatagccgcc tgtattgtaa tgacaacgtt tcgcggctat tcttgtattg tttggatgca
    33421 agaatggccc ccgtggtgtt gtcaggtgca tacctgcaac gtgcgggggt tttctctctc
    33481 cagcaaccaa tgccaccagg gattaagccc ccgcaacatt gcgcctcacc ggaatccttc
    33541 cggcttgacg ctgattctac gactgttttt ctgttccgga atccgtctca tcttcactct
    33601 gtgctgcgca ttccgcagaa tattatccgc ttgcaaccgt tgcatttttc ttacgaaaaa
    33661 ggccgcagag tttcactcct gcggcctggt ccgggcgcaa attgccatta cggcagcctg
    33721 acgcccacat gacacgctac ttccgcttgt tcagccagtt cacgatcata ctggcaagaa
    33781 tgccagcaat gaccggagcc gctaaatcat gccagaaggc catgcccagc tctgcgagcg
    33841 tcatatagcc gcctgtgttg taatgacaac gtttcgcggc tattcttgag tggtctaggt
    33901 tcaagattag cccccgttat gttgtcaggt atatacctgc aacgtgcggg ggttttctct
    33961 ctccagcaac caatgccacc agggataaag cccccgcaac attgcgcctc accggaaccc
    34021 ttccggcttg ccgctgattt tacgattgct ttttactgtc agcacccggc aatcttcctc
    34081 attgcgcgat gccttccagc ttaatttctt tatttgcaat agttgcagtt catatccggg
    34141 ctgactccag actggtagtg aaatgcgcaa ttttgtagaa ttgcgcctcc tcgcgacctc
    34201 cccagtgaat ctgttgcctc attttttaca tactgcgtat tcgacttctc cacctgttgc
    34261 gcaagagaaa ctgggtttat tcatttttgc gagaccgact tctttctgga caggacttta
    34321 tgcagcacaa cacactatcg aaacacaatc agaaattgcc gtttacacgc tacgacttcg
    34381 gctgggtttt attatgcata ggcatggcga ttggtgccgg aaccgtgctg atgccagtac
    34441 aaattggctt gaagggaatt tgggtattta ttaccgcagc gatcattgct tatcctgcca
    34501 cctgggtagt gcaggacatt tatttaaaaa ccctttctga aagcgattcc tgtaatgact
    34561 acaccgatat tatcagtcat tacctgggga agaactgggg aattttcctc ggggttatct
    34621 actttttgat gattatccac gggattttta tctactctct ctccgtggtt ttcgacagcg
    34681 cctcgtacct gaaaaccttc ggtttaaccg atgccgatct ttcacaatct ctactttata
    34741 aagtcgctat tttcgccgta ctggtggcga ttgcgtctgg tggtgaacga ttactgttta
    34801 agatttccgg gccaatggtg gtggtcaaag tagggattat tgtcgtgttc ggttttgcga
    34861 tgatcccgca ctggaatttc gccaatataa ccgccttccc gcaagcctcc gtctttttcc
    34921 gcgatgtcct gcttacaatt ccattttgct tcttttctgc ggtatttatt caggtactta
    34981 acccaatgaa tattgcctat cgtaaacggg aagcagataa agtactggca acccggctcg
    35041 cgctgcgtac ccaccgaatt agttatatca cgctcatcgc ggtgatcctg ttttttgcct
    35101 tttcgtttac cttctcaatt agccacgaag aagccgtttc tgcctttgaa caaaatatct
    35161 cggcactggc gctggccgcg caggtgatcc ctggacatat cattcatatc acctctacgg
    35221 tgctgaatat ctttgccgta ctgaccgcat tctttggcat ttatctcggt ttccacgagg
    35281 ctattaaagg cattattctc aatctgttaa gccgaattat tgataccaag aaaattaact
    35341 cacgcatgct gactctggcg atctgcgctt ttatcgtcat tacattgacg atttgggttt
    35401 cgtttcgtgt atcggtgctg gtgttctttc agttgggaag cccgttatat ggcattgtgt
    35461 cgtgcctcat tccgtttttc ctgatctata aagtcgcaca actggaaaaa cttcgcggat
    35521 ttaaagcctg gctaattctg ctttacggca ttttgctatg cttgtcgcca ctgttgaagc
    35581 tgattgagta aaccggagcg catggccccg gttttgtgag ttaacgctgc ggattttcat
    35641 cctgatcaac agcaaaacaa gcgaccagtt gaccgccgta gtcttttagc tgcggctgca
    35701 actgggtgca ggggccgaag cgccgacgac agcgggcgtt gaaggcgcaa cccggcggag
    35761 gattcagcgg gcttggcagt tcaccggtga gtttgatgcg ttcacgacga tcgtccgggt
    35821 tcaggcgtgg cgtcgcggag agcagcgcct gggtgtacgg atggcgcggg ttattgaaga
    35881 tttggtcctt cgttcctttc tccacgcagc ggcccaggta catcaccatc acttcatcag
    35941 caatgtgctc caccaccgac aggtcgtggg agataaagac ataagaaagc cccaactcct
    36001 gctgcaaatc catcatcaga ttcagcacct gcgcacgcac tgagacgtcc agcgcagaaa
    36061 ccggttcatc ggcaatcacc acgtccgggt cgagcatcag accacgggca atagcgatac
    36121 gctgacgttg tccgccggag aacatatgcg gatagcggtc atagtgctcg gttttcaggc
    36181 cgactttcgc catcatcgac agggcttttt cccgacgctg ttctttgctg aagctggtgt
    36241 taataagcag cggctcttcg agaatttgcc caactttttt acgcggattc agcgaaccgt
    36301 aagggttctg gaagacaatc tggattttct gccgacgcag cttctgcgcc tgcggatcgt
    36361 gcttgagcag atcctgcccc tggtaataca gctcaccacc ggtgggcgtt tcgatcatcg
    36421 tcagcaaccg accgagggtc gatttaccgc agccagattc gcccactact gccagcgttt
    36481 tgccacgttc aaggttaaac gaaacgccgt ccagcgcctt caccagacgt tccggcgcga
    36541 acatgccttt cttcaccgga taatgttttt tcaggtcgat agcctgcaac agcggttgtt
    36601 gcgaggtggc ctcttgcgta ctcatagtgt cggcctcccg gcatcatcaa gtgggtaatg
    36661 gcatttggac tgacgcccat cagcgagcat attcagcgcc ggttcttcag cgcgacatct
    36721 gtcagtggca taggggcagc gcgggttaag caggcagccg ttcgggcggt cgtacttacc
    36781 gggaacgaca cctggcaacg acgccagacg ttctttgtcc tgagcaaatt ctggcagcgc
    36841 acgcagcaat gcctgagtat acgggtgacg cggcgcatgg aagatggcgt gcgcatcacc
    36901 ggtttccacc acctggcctg catacatcac gatgatttta tgtgccgctt ccgccaccag
    36961 cgccaggtca tgggtaatta acaccagcgc catgttctct ttctgctgta gctccagcag
    37021 tagttcgatg atttgcgcct gaatggtcac gtccagcgcg gtggtcggtt catcggcaat
    37081 cagcagtttt ggccgacagg caatcgccat ggcgatcatc acgcgctggc tcatgccgcc
    37141 ggaaagctga tgcgggtaaa tatccagacg cgatgccgga tcgggaatac cgacctgatt
    37201 cagcaggtca atcgcccgct gacggcgagt gctcttatta ccgccctgat gcaccttaat
    37261 cgcttccatg atctggaaac ccacggtgta gcacgggtta aggctggtca tcgggtcctg
    37321 gaagatcatc gccacttcgg cacccaccag gttgcggcgc tctttttctg atatacgctg
    37381 caaatcctga ccgttaaaca ccagcttttc cgccattacg cggcccggat aatcaatcag
    37441 ccccataatc gccagtgaac tgaccgactt accggaacca gactcaccca caatcccgac
    37501 cacttcgccc tgttttacgc tgtagctgat gcggtctacg gcgcggaacg gtgcgctttc
    37561 gtcgccgaaa tgcaccgata atttatctac atttaataac gccatctcga acctcttact
    37621 gcttcagttt gggatcgagc gcgtcacgca gaccgtcacc catcaggtta aatgccagca
    37681 ccgtcagcag gatcgccaga cccgggaagg tcacgaccca ccaggcgctt tgcgcgaact
    37741 gcaacacgtc ggagagcatg gtgccccact ctggtgttgg cggctgcgct cccatgccga
    37801 ggaagccaag ggcggccata tcgagaatgg cgttagagaa accgagcgac gcctgaacaa
    37861 tcagcggcgc aaggcagttc gggaagatgt taataaacat ctgacgcatc gccccggcac
    37921 ccgccacgcg agacgcggtg acgtaatcgc ggttaacttc caccagcacg gcggcgcggg
    37981 ttaagcgcac atagtgcggc aaggcaacga aggtcagtgc cagcgcggcg ttaccaatcg
    38041 acgggccgaa gattgccacc agcaccagcg ccagcagcag acttggcagc gccagcatga
    38101 tatcgaccac gcgcataatg atgttatcga ccaggccgcc aaagtaaccg gcgatcaggc
    38161 cgagaataac gcccataatc agcgataaca caactaccag acagccaacc agcagcgaca
    38221 ggcgcgcacc gtacatcagg cgcgacagca catcacggcc tacgtcatcg gtgcccagca
    38281 agtgcgccat gctgccgcct tcctgccagg ctggcggggc gagcagtgca tcgcggaact
    38341 gttccgccgg gttatagggt gcaatccagt tggcaaagat cgcgatgaac agcacgatga
    38401 cgacgtaaac cagcccgacg accgcgcctt tgttgcgttt aaaatagtgc cagaactcct
    38461 gtaacggggt catcggcacc ggtgcgctaa tcactttatt ttcagtaacc tgtgacatga
    38521 tgtcccctta cttcttatga cgaatacgcg ggttcaccac gccgtacagc agatcgacca
    38581 gcaagttgac gaggataatc atcgtcgcca ccagcaatac gccgccctgc actaccggat
    38641 agtcgcggcg ttgcagcgcg tcaatcaacc agcgccccag accgggccac gagaagatgg
    38701 tttcggtcag aatcgccccc gccagcaatg ttcccacctg caggccgata acggtcacta
    38761 ccggcagcat cgcgttacgc agcgcatgga cgataatcac ccgcatgcgg gttagccctt
    38821 tggcgcgcgc ggtgcggatg taatcctcgc ccagcacttc cagcatcgag gagcgtgtca
    38881 tacgcacaat gaccgccagc ggaatagtac ccagcacaat ggcaggcaag atcatatggg
    38941 cgacggcatc gataaagttg ccgtcttcac cccagatggc ggtgtcgatt agcataaaac
    39001 cggttaacgg attggagtca tcgaggaaca ccatatcgct cacgcgaccg gagacgggcg
    39061 tcaggttcca gtgcaccgaa accagcatga tcagcatcat gccccaccag aagatcggca
    39121 tcgagtagcc ggtcagcgcc aggccaaccg ctgtgtgatc gaaaatggaa ccgcgtttaa
    39181 ccgcagccag cacgccgacc ggaataccga ccgcagtcgc aaaaatcatc gcgcagacgc
    39241 caagttccag cgtggcctgg aggcgcggca cgaactcttc ccaaaccggg atgcggcttt
    39301 tcattgaaat gcctagatcg ccatgcataa cgccccaaat gtaatggaga tactgctgcc
    39361 acatcggttt atctaagccg agttcagcca gcagctgcgc gtgacgctct ggggagatcc
    39421 cacgttcgcc cgccatgatc atcaccggat cgcccgggat catgtggaca aaggcaaatg
    39481 tgagaagggt aataccgata aacgtgggga tgacgagtcc caaacgtcgg agaataaact
    39541 gcaacataac ccggattctc tatagtgacc cgcagcctgg aactgcgtgt ctgtattgct
    39601 cacaaatcaa ttcccgccta ggtcgggctg ggaatgaagt attgctgccg gatggcgctg
    39661 cacgcagagg cacagcgcca ccgggtatgg cttttaatta ttcgatagag acgttttcga
    39721 agtgatgttt gcctaatgga tcaaccacat agcctttaac ttctttgcgt accggttcaa
    39781 acacggtgga gtgagcgatg atcagtgccg gagcctgatc gtgcatcacc acctgcgctt
    39841 gcttgtacag ttcaacgcgt ttgttatggt cgtcggtagc acgcgccggt tgaatcagat
    39901 cttcaaacgg tttgtagcac cattttgagt agttggagcc ttgttcagag gcggcgcagc
    39961 tgaacagggt ggcgaagaag ttatccggat ccccgttatc gccagtccag cccatcatta
    40021 ccgtctggtg ctcgccatct ttcgcacgct tgaggtactc accccattcg taggtgacaa
    40081 ttttggcctg cacgccgact ttcgcccagt cagcctgaat catctccgcc atgcggcgag
    40141 cgttcgggtt atatgggcgc tgtaccggca tcgcccacag gtcgatggag aaaccttttt
    40201 ccagacccgc ttctttcagc aaggctttcg ctttttcagg atcgtaggtg taatcctgaa
    40261 cgtcgtcgtt atagccccac atggttggcg ggatcaggtt tttcgctgat acacccgcgc
    40321 cctgataaac cgctttgatg atcgcgtctt tgttcaccgc gtaggtcagt gcctggcgaa
    40381 ctttcacgtc atccagcggt tttttctgca cgttatagga gaggtagccg acgttcagcc
    40441 ccggcatttc catcaggttg atggatttat cctgcttcat gcgagcgatg tctgccgggt
    40501 tcgggtacgg catcacctgg cattcattct tctgcaattt cgcataacgc acggaagcgt
    40561 caggagtaat agagaagacc agcgtatcga tctgcggttt ggtgccccag tagccatcaa
    40621 acgctttgta gcggatacgg gagtcttttt ggtactgctg taactggaac ggaccggtgc
    40681 cgattgggtt gaggtccagt ttttccggtg taccggcttt catcatcgcg tcagcatatt
    40741 cttttgacag aatagaggcg aagtccattg ccaggtcagc gaggaacggc gcttccgggc
    40801 gagtcagcac aaactgaacg gtgttgtcgt ccaccttttt cacttcgctg atcagctccg
    40861 gcaagcccat gccttcgaag tattcgtagc tgccgccaga aactttatgg tacgggtttt
    40921 gcgcgttttt ctgacgatca aacgagaaca ccacgtcatc ggcgttcagt tcacgcgtcg
    40981 gtttgaattc tttattgtcg tgccacttca cacctttacg cagatggaag gtataggttt
    41041 taccgtcttc gctgacttcc cacttttcag cgaggcccgg gatcacttca gtggtgccga
    41101 ttttaaattc aaccaggcgg ttataaagcg gtacggaaga ggcgtcatag gtggtgccgg
    41161 aggtaaacag ctgcgggtta aacccttccg gagatccttc tgagcaataa accagagttt
    41221 tagcctgaac acttgctgcg acggtcatag ccaccaggct gagaccaagc ttcagcatcc
    41281 ctgacttttt caaggaaata cgcattattc tgctccaatt gtgatgtttg ttgttttaac
    41341 cctttgcagt gggttgtcgc tgcctgacct tttttgtttt ttgcccggtc gggtcaacgt
    41401 tatgaggtgg ggatgccgta ctaattaaca tcagtgtaac agtgcggatc ctccataaaa
    41461 tgcccctcgt gacctacaat ctgtcaacag aatgtgaaaa cgtcaataca gccaaccggg
    41521 atttacacca acggtgagaa tccacaaaca aagattaaaa aaacttcaaa cagctatttg
    41581 cagcagagaa atttgtgcta ctccaacatg accagaacaa tcagcttaat atttagcaac
    41641 atttggtgat taagttttat gcatttaatg aaaaaaatct gaggaaaagg tggatatctg
    41701 agcgattaat accatgaacg ctaaaacgca cagcggaaaa tgccagcgcc agccataagt
    41761 aacgcaaaaa aagatttaag caaatataaa aaaagacaat ggtttctgtc acaaatccgt
    41821 taatggatag tgagatatgg ggcgcaaagt tggcaggaaa atgtgtggtg tgttggtcga
    41881 ataaggcggt cgcgccgcat ccgacaataa gtgcggtgcc gttgcctgat gcgacgctg
//
LOCUS       DAZFWL020000024        41456 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFWL020000024 DAZFWL020000000
VERSION     DAZFWL020000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 41456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.024"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..202)
                     /locus_tag="ACHKNP_RS07210"
                     /old_locus_tag="pgaptmp_001442"
     CDS             complement(<1..202)
                     /locus_tag="ACHKNP_RS07210"
                     /old_locus_tag="pgaptmp_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703638.1"
                     /translation="MSGKPAARQGDMTQYGSSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALP"
     gene            complement(362..574)
                     /gene="rpmE"
                     /locus_tag="ACHKNP_RS07215"
                     /old_locus_tag="pgaptmp_001443"
     CDS             complement(362..574)
                     /gene="rpmE"
                     /locus_tag="ACHKNP_RS07215"
                     /old_locus_tag="pgaptmp_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HGU4703639.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            777..2975
                     /gene="priA"
                     /locus_tag="ACHKNP_RS07220"
                     /old_locus_tag="pgaptmp_001444"
     CDS             777..2975
                     /gene="priA"
                     /locus_tag="ACHKNP_RS07220"
                     /old_locus_tag="pgaptmp_001444"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGU4703640.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3131..4156
                     /gene="cytR"
                     /locus_tag="ACHKNP_RS07225"
                     /old_locus_tag="pgaptmp_001445"
     CDS             3131..4156
                     /gene="cytR"
                     /locus_tag="ACHKNP_RS07225"
                     /old_locus_tag="pgaptmp_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HGU4703641.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4248..5207
                     /gene="ftsN"
                     /locus_tag="ACHKNP_RS07230"
                     /old_locus_tag="pgaptmp_001446"
     CDS             4248..5207
                     /gene="ftsN"
                     /locus_tag="ACHKNP_RS07230"
                     /old_locus_tag="pgaptmp_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HGU4703642.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5300..5830
                     /gene="hslV"
                     /locus_tag="ACHKNP_RS07235"
                     /old_locus_tag="pgaptmp_001447"
     CDS             5300..5830
                     /gene="hslV"
                     /locus_tag="ACHKNP_RS07235"
                     /old_locus_tag="pgaptmp_001447"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HGU4703643.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5840..7171
                     /gene="hslU"
                     /locus_tag="ACHKNP_RS07240"
                     /old_locus_tag="pgaptmp_001448"
     CDS             5840..7171
                     /gene="hslU"
                     /locus_tag="ACHKNP_RS07240"
                     /old_locus_tag="pgaptmp_001448"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HGU4703644.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7238..8164
                     /gene="menA"
                     /locus_tag="ACHKNP_RS07245"
                     /old_locus_tag="pgaptmp_001449"
     CDS             7238..8164
                     /gene="menA"
                     /locus_tag="ACHKNP_RS07245"
                     /old_locus_tag="pgaptmp_001449"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HGU4703645.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8257..8742
                     /gene="rraA"
                     /locus_tag="ACHKNP_RS07250"
                     /old_locus_tag="pgaptmp_001450"
     CDS             8257..8742
                     /gene="rraA"
                     /locus_tag="ACHKNP_RS07250"
                     /old_locus_tag="pgaptmp_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HGU4703646.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8827..9072)
                     /gene="zapB"
                     /locus_tag="ACHKNP_RS07255"
                     /old_locus_tag="pgaptmp_001451"
     CDS             complement(8827..9072)
                     /gene="zapB"
                     /locus_tag="ACHKNP_RS07255"
                     /old_locus_tag="pgaptmp_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HGU4703647.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9497..10342
                     /gene="glpF"
                     /locus_tag="ACHKNP_RS07260"
                     /old_locus_tag="pgaptmp_001452"
     CDS             9497..10342
                     /gene="glpF"
                     /locus_tag="ACHKNP_RS07260"
                     /old_locus_tag="pgaptmp_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HGU4703648.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10365..11873
                     /gene="glpK"
                     /locus_tag="ACHKNP_RS07265"
                     /old_locus_tag="pgaptmp_001453"
     CDS             10365..11873
                     /gene="glpK"
                     /locus_tag="ACHKNP_RS07265"
                     /old_locus_tag="pgaptmp_001453"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGU4703649.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12008..13018
                     /gene="glpX"
                     /locus_tag="ACHKNP_RS07270"
                     /old_locus_tag="pgaptmp_001454"
     CDS             12008..13018
                     /gene="glpX"
                     /locus_tag="ACHKNP_RS07270"
                     /old_locus_tag="pgaptmp_001454"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU4703650.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13115..13861
                     /gene="fpr"
                     /locus_tag="ACHKNP_RS07275"
                     /old_locus_tag="pgaptmp_001455"
     CDS             13115..13861
                     /gene="fpr"
                     /locus_tag="ACHKNP_RS07275"
                     /old_locus_tag="pgaptmp_001455"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HGU4703651.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13866..14294)
                     /gene="uspD"
                     /locus_tag="ACHKNP_RS07280"
                     /old_locus_tag="pgaptmp_001456"
     CDS             complement(13866..14294)
                     /gene="uspD"
                     /locus_tag="ACHKNP_RS07280"
                     /old_locus_tag="pgaptmp_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HGU4703652.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14321..14620)
                     /gene="yiiS"
                     /locus_tag="ACHKNP_RS07285"
                     /old_locus_tag="pgaptmp_001457"
     CDS             complement(14321..14620)
                     /gene="yiiS"
                     /locus_tag="ACHKNP_RS07285"
                     /old_locus_tag="pgaptmp_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HGU4703653.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14832..15272)
                     /gene="yiiR"
                     /locus_tag="ACHKNP_RS07290"
                     /old_locus_tag="pgaptmp_001458"
     CDS             complement(14832..15272)
                     /gene="yiiR"
                     /locus_tag="ACHKNP_RS07290"
                     /old_locus_tag="pgaptmp_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4703654.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15373..15972
                     /gene="yiiQ"
                     /locus_tag="ACHKNP_RS07295"
                     /old_locus_tag="pgaptmp_001459"
     CDS             15373..15972
                     /gene="yiiQ"
                     /locus_tag="ACHKNP_RS07295"
                     /old_locus_tag="pgaptmp_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HGU4703655.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16080..16847
                     /gene="tpiA"
                     /locus_tag="ACHKNP_RS07300"
                     /old_locus_tag="pgaptmp_001460"
     CDS             16080..16847
                     /gene="tpiA"
                     /locus_tag="ACHKNP_RS07300"
                     /old_locus_tag="pgaptmp_001460"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGU4703656.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16902..17657)
                     /gene="cdh"
                     /locus_tag="ACHKNP_RS07305"
                     /old_locus_tag="pgaptmp_001461"
     CDS             complement(16902..17657)
                     /gene="cdh"
                     /locus_tag="ACHKNP_RS07305"
                     /old_locus_tag="pgaptmp_001461"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HGU4703657.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(17764..18753)
                     /gene="sbp"
                     /locus_tag="ACHKNP_RS07310"
                     /old_locus_tag="pgaptmp_001462"
     CDS             complement(17764..18753)
                     /gene="sbp"
                     /locus_tag="ACHKNP_RS07310"
                     /old_locus_tag="pgaptmp_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HGU4703658.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLV
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19072..20034)
                     /gene="pfkA"
                     /locus_tag="ACHKNP_RS07315"
                     /old_locus_tag="pgaptmp_001463"
     CDS             complement(19072..20034)
                     /gene="pfkA"
                     /locus_tag="ACHKNP_RS07315"
                     /old_locus_tag="pgaptmp_001463"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGU4703659.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20215..21117)
                     /gene="fieF"
                     /locus_tag="ACHKNP_RS07320"
                     /old_locus_tag="pgaptmp_001464"
     CDS             complement(20215..21117)
                     /gene="fieF"
                     /locus_tag="ACHKNP_RS07320"
                     /old_locus_tag="pgaptmp_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HGU4703660.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21266..21766)
                     /gene="cpxP"
                     /locus_tag="ACHKNP_RS07325"
                     /old_locus_tag="pgaptmp_001465"
     CDS             complement(21266..21766)
                     /gene="cpxP"
                     /locus_tag="ACHKNP_RS07325"
                     /old_locus_tag="pgaptmp_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HGU4703661.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21916..22614
                     /gene="cpxR"
                     /locus_tag="ACHKNP_RS07330"
                     /old_locus_tag="pgaptmp_001466"
     CDS             21916..22614
                     /gene="cpxR"
                     /locus_tag="ACHKNP_RS07330"
                     /old_locus_tag="pgaptmp_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HGU4703662.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22611..23984
                     /gene="cpxA"
                     /locus_tag="ACHKNP_RS07335"
                     /old_locus_tag="pgaptmp_001467"
     CDS             22611..23984
                     /gene="cpxA"
                     /locus_tag="ACHKNP_RS07335"
                     /old_locus_tag="pgaptmp_001467"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HGU4703663.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24126..24800)
                     /gene="yiiM"
                     /locus_tag="ACHKNP_RS07340"
                     /old_locus_tag="pgaptmp_001468"
     CDS             complement(24126..24800)
                     /gene="yiiM"
                     /locus_tag="ACHKNP_RS07340"
                     /old_locus_tag="pgaptmp_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HGU4703664.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24949..25932)
                     /gene="kdgT"
                     /locus_tag="ACHKNP_RS07345"
                     /old_locus_tag="pgaptmp_001469"
     CDS             complement(24949..25932)
                     /gene="kdgT"
                     /locus_tag="ACHKNP_RS07345"
                     /old_locus_tag="pgaptmp_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HGU4703665.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26192..26812)
                     /gene="sodA"
                     /locus_tag="ACHKNP_RS07350"
                     /old_locus_tag="pgaptmp_001470"
     CDS             complement(26192..26812)
                     /gene="sodA"
                     /locus_tag="ACHKNP_RS07350"
                     /old_locus_tag="pgaptmp_001470"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HGU4703666.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27097..28131
                     /gene="rhaT"
                     /locus_tag="ACHKNP_RS07355"
                     /old_locus_tag="pgaptmp_001471"
     CDS             27097..28131
                     /gene="rhaT"
                     /locus_tag="ACHKNP_RS07355"
                     /old_locus_tag="pgaptmp_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HGU4703667.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWSNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28128..28976)
                     /gene="rhaR"
                     /locus_tag="ACHKNP_RS07360"
                     /old_locus_tag="pgaptmp_001472"
     CDS             complement(28128..28976)
                     /gene="rhaR"
                     /locus_tag="ACHKNP_RS07360"
                     /old_locus_tag="pgaptmp_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HGU4703668.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQHVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLAMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(29050..29887)
                     /gene="rhaS"
                     /locus_tag="ACHKNP_RS07365"
                     /old_locus_tag="pgaptmp_001473"
                     /pseudo
     CDS             complement(29050..29887)
                     /gene="rhaS"
                     /locus_tag="ACHKNP_RS07365"
                     /old_locus_tag="pgaptmp_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            30175..31644
                     /gene="rhaB"
                     /locus_tag="ACHKNP_RS07370"
                     /old_locus_tag="pgaptmp_001474"
     CDS             30175..31644
                     /gene="rhaB"
                     /locus_tag="ACHKNP_RS07370"
                     /old_locus_tag="pgaptmp_001474"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HGU4703669.1"
                     /translation="MTFRNCVAVNLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDNF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31641..32900
                     /gene="rhaA"
                     /locus_tag="ACHKNP_RS07375"
                     /old_locus_tag="pgaptmp_001475"
     CDS             31641..32900
                     /gene="rhaA"
                     /locus_tag="ACHKNP_RS07375"
                     /old_locus_tag="pgaptmp_001475"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HGU4703670.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARQALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            32983..33807
                     /gene="rhaD"
                     /locus_tag="ACHKNP_RS07380"
                     /old_locus_tag="pgaptmp_001476"
     CDS             32983..33807
                     /gene="rhaD"
                     /locus_tag="ACHKNP_RS07380"
                     /old_locus_tag="pgaptmp_001476"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HGU4703671.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAEVLVKIYSMGGMKQTITREELIALGERFGVTPLASALAL"
     gene            33879..35027
                     /gene="fucO"
                     /locus_tag="ACHKNP_RS07385"
                     /old_locus_tag="pgaptmp_001477"
     CDS             33879..35027
                     /gene="fucO"
                     /locus_tag="ACHKNP_RS07385"
                     /old_locus_tag="pgaptmp_001477"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HGU4703672.1"
                     /translation="MSFMLALPKISLHGAGAIGDMVNLVAHKQWGKALIVTDGQLVKL
                     GLLDSLFIALDQYEMSYHLFDEVFPNPTEELVQKGYAAFQDAACDYIIAFGGGSPIDT
                     AKAVKILTANPAPSTAYSGVGKVTNPGVPLVAINTTAGTAAEMTSNAVIIDTARQVKE
                     VIIDPNIIPDIAVDDASVMLEIPASVTAATGMDALTHAVEAYVSVGAHPLTDANALEA
                     IRLITLWLPKAVDDGHNLEAREQMAFAQYLAGMAFNSAGLGLVHALAHQPGATHNLPH
                     GVCNAILLPVIENFNRPNAIARFARIAQAMGVDTRDMSEEAASTEAINAIRALSHRVG
                     IPAGFSQLGITKEDIEGWLDKALADPCVPCNPRTASRDEVRELYLEAL"
     gene            35024..35338
                     /gene="rhaM"
                     /locus_tag="ACHKNP_RS07390"
                     /old_locus_tag="pgaptmp_001478"
     CDS             35024..35338
                     /gene="rhaM"
                     /locus_tag="ACHKNP_RS07390"
                     /old_locus_tag="pgaptmp_001478"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HGU4703673.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            35651..36097
                     /gene="frvA"
                     /locus_tag="ACHKNP_RS07395"
                     /old_locus_tag="pgaptmp_001479"
     CDS             35651..36097
                     /gene="frvA"
                     /locus_tag="ACHKNP_RS07395"
                     /old_locus_tag="pgaptmp_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HGU4703674.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFINQLRQGDADQVLALLNQTLSS"
     gene            36108..37559
                     /gene="frvB"
                     /locus_tag="ACHKNP_RS07400"
                     /old_locus_tag="pgaptmp_001480"
     CDS             36108..37559
                     /gene="frvB"
                     /locus_tag="ACHKNP_RS07400"
                     /old_locus_tag="pgaptmp_001480"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HGU4703675.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            37549..38619
                     /gene="frvX"
                     /locus_tag="ACHKNP_RS07405"
                     /old_locus_tag="pgaptmp_001481"
     CDS             37549..38619
                     /gene="frvX"
                     /locus_tag="ACHKNP_RS07405"
                     /old_locus_tag="pgaptmp_001481"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4703676.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPQSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAKH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYFPNQKLVAALKSCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVSAFSQFRQVD"
     gene            38619..40367
                     /gene="frvR"
                     /locus_tag="ACHKNP_RS07410"
                     /old_locus_tag="pgaptmp_001482"
     CDS             38619..40367
                     /gene="frvR"
                     /locus_tag="ACHKNP_RS07410"
                     /old_locus_tag="pgaptmp_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HGU4703677.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(40417..>41456)
                     /gene="yiiG"
                     /locus_tag="ACHKNP_RS07415"
                     /old_locus_tag="pgaptmp_001483"
     CDS             complement(40417..>41456)
                     /gene="yiiG"
                     /locus_tag="ACHKNP_RS07415"
                     /old_locus_tag="pgaptmp_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HGU4703678.1"
                     /translation="KRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPE
                     APVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQ
                     GPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTI
                     NEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELK
                     NIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKG
                     GMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREY
                     NEMVDDYNSLR"
BASE COUNT        10069 a        10722 c        10730 g         9935 t
ORIGIN      
        1 cgggcagggc gatgtcggtt tcaccgggaa ggacctttgc accgagcagc ggattgaccg
       61 gatggccgga cgtcaccccg ccggggcaca ccgaacaggc cacgccggtg ggggcaccga
      121 tgcgcacccc ggctgaaccc tggacaatgc tactgccata ctgcgtcatg tcgccctgac
      181 gcgctgccgg ttttccgctc atcgtaaaat ccttttgaaa taaataagaa ccagaagcct
      241 agcgccagtc aacaacagga aatgataatg agttaacacc ccggaatttt tcagaattta
      301 taaaaggcaa attacaggca caaaaaaagc gccgtgcggc gcttttttcg gaaatccggt
      361 cttatttgct gcccgggatg ttgaaacgct tgttgaagcg gtcaacacgg ccaccggtag
      421 caacatcacg ctgtttgcca gtgaagaacg ggtggcactt gctgcacacg tcgaggttca
      481 ggtcatgacc aacggtggag cggattttca ttacgttacc gcaagagcag ctagcagtaa
      541 tttcttcgta tttcgggtga atatcttttt tcatgggaaa acctcggttt aaggccgcgt
      601 cgctcttcca gccctaacgc cagacaccac gcgatgttaa aagtatagct tcaatacgat
      661 catttcgtac gaaggcgcga aatcatacag aaattaacca gcgtatgcaa actgatccgc
      721 actcttctac ggcaatgtgt atactaaccc accgaatttc aagtcaggat gatgctatgc
      781 ccgtagccca cgttgccttg cccgttccgc ttcctcgtac ctttgactat ctgctgccag
      841 aaggcatgac ggttaaagct gggtgtcgcg tgcgcgtgcc gtttggcaaa cagcaggagc
      901 gcatcgggat tgtggtatca gttagcgatg ccagcgaact gccgctcaat gagctaaaag
      961 cggtagtcga agtgctggac gttgagccgg tatttactca ctccgtctgg cggttactgc
     1021 tctgggcggc agattactat catcatccga ttggcgatgt gctgtttcat gccttgccga
     1081 ttttactgcg ccaggggcgg cctgcggcga acgcgccgat gtggtactgg tttgccactg
     1141 aacaaggtca ggcggtggat cttaacagcc tgaaacgttc gccaaagcaa caacaggcgc
     1201 tggcggcgtt acggcaaggc aaaatctggc gcgaccaggt cgcggaactt gaatttaatg
     1261 atgccgcgtt gcaggcgctg cgcaaaaaag gtctgtgtga tttagcaagt gaaacaccag
     1321 agtttagcga ctggcgaacg aactatgccg tttctggtga gcggttgcga ttgaataccg
     1381 aacaagccac cgccgttggc gcaattcata gcgcggcaga tactttttct gcctggctgc
     1441 tggcgggcgt taccggttcc ggtaaaacgg aggtttatct cagtgtactg gaaaacgtgc
     1501 tcgctcaggg caaacaggcg ctggtgatgg tgccggaaat cggcctgaca ccgcaaacta
     1561 tcgcccgttt tcgtgaacgt tttaatgccc ccgtggaagt tctgcattcc ggcctgaacg
     1621 acagcgagcg gctttcggcg tggttgaaag cgaaaaatgg cgaggcggcg attgtgatcg
     1681 gcacccgctc cgcgctgttt acgccgttta aaaatctcgg cgtgattgtc attgatgaag
     1741 agcacgacag ctcctacaag cagcaggaag gctggcgcta tcatgcccgc gacctggcgg
     1801 tgtatcgtgc gcatagcgag caaatcccga ttattcttgg ctccgcaacg cccgcgctgg
     1861 aaacgttatg caacgtccag cagaaaaaat accgcctgct gcgcctgacc cgtcgggcag
     1921 ggaatgcgcg tccggcaatt caacatgtgc tggatttaaa aggtcagaag gtgcaggcag
     1981 gtctggctcc ggcgttaatc acccgtatgc gccagcattt acaggccaac aatcaggtta
     2041 tcctctttct taatcgtcgg ggttttgcgc ctgcgctgct gtgccacgac tgcggctgga
     2101 tagccgaatg cccacgttgc gatcactact acacgctgca tcaggcgcaa cagcatttgc
     2161 gctgtcacca ctgtgacagt cagcgtccgg tgccgcgcca gtgcccttcc tgcggttcca
     2221 cgcacctggt ccccgtgggg ctgggcaccg aacagcttga acagacgctt gcgccgatgt
     2281 tccccggcgt gcccatttca cgtatcgacc gcgataccac cagccgcaaa ggggcgctgg
     2341 aacagcaact ggcggaagtg catcgcggcg gcgcgaggat cttgattggc acgcaaatgc
     2401 tggcgaaagg tcaccacttt ccggatgtga cgctggtggc gttactggat gtggacggcg
     2461 cgctgttttc tgccgatttt cgctcggcag aacgtttcgc ccagctttac acccaggtcg
     2521 ccggtcgtgc cgggcgtgcg ggtaaacagg gcgaagtggt gctgcaaacg caccatccgg
     2581 aacatcctct gttgcaaacg ttgctctata aaggctacga cgcctttgcc gaacaggcgc
     2641 tggctgagcg acgcatgatg cagctgccgc cgtggaccag ccatgtgatt gtgcgtgcgg
     2701 aagatcataa caatcagcat gcgccgctgt tcctgcaaca actgcgtaat ctgatccttt
     2761 ccagcccgct ggcagacgac aaactgtggg ttctcggccc ggttccggct ctggcaccta
     2821 agcgtggtgg ccgctggcgc tggcagatct tgttgcagca cccttcccgc gtgcgcttgc
     2881 aacacatcat tagtggtacg ctggcgctta ttaatacaat accggattcc cgcaaggtga
     2941 aatgggtgtt ggatgtcgat cccatcgaag gctaatgcgc aaacgttgcg aggcggatcg
     3001 aaaaattcaa tattcatcac acttttcatg aaaattctgt aaccgttttc acgcgctatc
     3061 tgctaaaaat gttgccgatg tgaagtaaac atggatgtag tacgcctgac gtgccaggcg
     3121 aggagtgagt gtgaaagcga agaagcagga aactgccgcg accatgaaag acgttgccct
     3181 caaggcaaaa gtctctacag cgactgtctc ccgagcatta atgaatcccg ataaagtctc
     3241 ccaggccacc cgtaatcggg ttgaaaaagc ggcccgggaa gtgggttatt taccgcagcc
     3301 tatggggcgc aacgtcaagc gtaatgaatc ccgcaccatt ctggtgattg tcccggatat
     3361 ctgcgatccc ttctttagcg aaattattcg cggtatcgaa gttacggcgg caaatcacgg
     3421 atatctggtg ctgattggcg actgtgcgca tcaaaatcag caggaaaaaa cctttatcga
     3481 tttgatcatc accaagcaaa ttgatggcat gttgctgctg ggttcaaggc tgccgtttga
     3541 tgccagcatt gaggaacagc gtaatctgcc gccgatggtg atggcgaacg aattcgcacc
     3601 ggaactggag ctacctacag ttcatatcga caatctgacc gccgcatttg atgcagtaaa
     3661 ttatctatat gagcaagggc ataaacggat tggctgtata gccggtcccg aagagatgcc
     3721 gctgtgtcac taccgcctgc aaggttacgt tcaggcgctg cgtcgctgcg gcattatggt
     3781 tgatccgcaa tacatcgccc gtggcgactt caccttcgaa gccggaagca aagcgatgca
     3841 gcagctgctt gatcttccac aaccgcctac tgctgtcttc tgccatagcg atgtgatggc
     3901 gctcggcgca ctttctcagg caaaacgcca ggggctgaaa gtcccggaag acctttccat
     3961 aatcggtttt gataacatcg acctgacgca attttgtgat ccgccgctga caaccatcgc
     4021 gcagccgcgt tacgaaatcg gtcgggaagc tatgctgtta ttgcttgatc aaatgcaggg
     4081 gcaacacgtt ggcagtggct ctcgtttaat ggactgcgaa cttatcatcc ggggatcaac
     4141 acgcgcgtta ccttaaagta aacctttaag actgccgtga ctggtcaaag gcccgccgct
     4201 taagtaacat ggcgggctga cgaacgaata aatacagcga aacgatagtg gcacaacgag
     4261 attatgtacg ccgcagccaa ccggcacctt cgcggcgaaa aaagagcacc tcacggaaaa
     4321 agcaacgaaa tctgcctgcg gtttctcccg ctatggtcgc tattgctgcc gccgttcttg
     4381 tgacctttat cggtggtctg tacttcatta cgcatcacaa gaaagaagag tccgagacgc
     4441 tgcaaagcca gaaagtgacc ggtaacggac taccaccaaa accagaagaa cgctggcgct
     4501 acattaaaga gctggaaagt cgccagccgg gtgtgcgtgc gcccacagaa ccttctgccg
     4561 gtggtgaagt gaaaacgccg gagcaactga caccagaaca acgtcagctt cttgaacaaa
     4621 tgcaggctga tatgcgccag cagccaacgc agctggttga agttccgtgg aatgaacaga
     4681 cgccggaaca gcgtcagcaa acgctacaac gccagcgtca ggcgcagcaa ttagcggagc
     4741 agcaacgcct ggctcagcag tcgcgtacga ctgaacaaag ctggcagcag cagacgcgta
     4801 cgtcgcaagc cgcgccggta caggcgcaac cgcgccagtc taaaccggct tctactcagc
     4861 agccgtacca ggatctgctg caaacccctg cgcacacgac cgcgcagtcg aagccgcagc
     4921 aagctgcgcc ggttgctcgt gccgctgacg cgccaaaacc gacggcggag aaaaaagacg
     4981 aacgccgctg gatggtgcag tgcggttcgt tcagaggcgc ggaacaggca gagacggtac
     5041 gtgctcagct ggcgttcgaa ggatttgact cgaaaatcac caccaacaat ggctggaatc
     5101 gtgtggtcat tggcccggtg aaaggcaaag agaacgcaga cagcaccctc aatcggttga
     5161 agatggcggg tcatacaaac tgcattcggc tcgccgccgg gggttgaaac cctcaaaatc
     5221 ccccccatct ataattgcat tatgccccgt acttttgtac ggggtttgta ctctgtattc
     5281 gtaaccaagg ggtcagctcg tgacaactat agtaagcgta cgccgtaacg gccatgtggt
     5341 catcgctggt gatggtcagg ccacgttggg caataccgta atgaaaggca acgtgaaaaa
     5401 ggtccgccgt ctgtacaacg acaaagtcat cgcgggcttt gcgggcggta ctgcggatgc
     5461 ttttacgctg ttcgaactgt ttgaacgtaa actggaaatg catcagggcc atctggtcaa
     5521 agccgccgtt gagctagcga aagactggcg taccgatcgc atgctgcgca aacttgaagc
     5581 actgctggca gtcgcggatg aaactgcatc gcttatcatc accggtaacg gtgacgtggt
     5641 gcagccagaa aacgatctta ttgctatcgg ctccggcggc ccttacgccc aggctgcggc
     5701 gcgtgcactg ttagaaaata ctgaactgag tgctcgtgaa attgctgaaa aggcgttgga
     5761 tattgcaggc gacatttgca tctataccaa ccatttccac accatcgaag aattaagcta
     5821 caaagcgtaa ggatctccca tgtctgaaat gaccccacgc gaaatcgtca gcgaactgga
     5881 taagcacatc atcggccagg acaacgccaa gcgttctgtg gcgattgctc tgcgtaaccg
     5941 ctggcgtcgc atgcagctca acgaagagct gcgccatgaa gtgaccccga aaaatatcct
     6001 gatgatcggc ccgaccggtg tcggtaaaac tgaaatcgcc cgtcgtctgg ctaagctggc
     6061 gaatgcgccg ttcatcaaag ttgaagcgac caaattcacc gaagtaggct acgtcggtaa
     6121 ggaagtggat tctattattc gcgatctgac cgatgccgcc gtgaaaatgg tacgcgtcca
     6181 ggctatcgag aaaaaccgtt atcgcgctga agaactggca gaagaacgta ttctcgacgt
     6241 gctgatccca cctgctaaaa acaactgggg acagaccgaa cagcagcagg aaccgtccgc
     6301 tgctcgtcag gcattccgca aaaaactgcg tgaaggccag cttgatgaca aagaaatcga
     6361 gatcgatctt gccgcagcac cgatgggcgt tgaaattatg gctcctccag gcatggaaga
     6421 gatgaccagc cagctgcagt ccatgttcca gaacctgggc ggccagaagc aaaaagcgcg
     6481 taagctgaaa atcaaagacg ccatgaagct gctgattgaa gaagaagcgg cgaaactggt
     6541 gaacccggaa gagctgaagc aagacgctat cgacgctgtt gagcagcacg ggatcgtgtt
     6601 tatcgacgaa atcgacaaaa tctgtaagcg cggcgagtct tccggtccgg atgtttctcg
     6661 tgaaggcgtt cagcgtgacc tgctgccgct ggtagaaggt tgcaccgttt ccaccaaaca
     6721 cgggatggtc aaaactgacc acattctgtt tatcgcttct ggcgcgttcc agattgcgaa
     6781 accgtctgac ctaatcccgg aactgcaagg tcgtctgcca atccgcgttg aactgcaggc
     6841 actgaccacc agcgacttcg agcgtattct gaccgagccg aatgcctcta tcaccgtgca
     6901 gtacaaagca ctgatggcga ctgaaggcgt aaatatcgag tttaccgact ccggtattaa
     6961 gcgcatcgcg gaagcggcat ggcaggtgaa cgaatctacc gaaaacatcg gtgctcgtcg
     7021 tttacacact gttctggagc gtttaatgga agagatttcc tacgacgcca gcgatttaag
     7081 cggtcaaaat atcactattg acgcagatta tgtgagcaaa catctggatg cgttggtggc
     7141 agatgaagat ctgagccgtt ttatcctata atcgcgttca atcattttca tcattgtttg
     7201 atggggctga aaggccccat ttttattggc gcgtattatg actgaacaac aaattagccg
     7261 aactcaggcg tggctggaaa gtttacgacc taaaaccctc cccctcgcct ttgctgcaat
     7321 tatcgtcggg acggcgctgg catggtggca aggtcacttc gatccgctgg tcgccctgct
     7381 ggcactaatt accgccgggc tattacagat cctttctaac ctcgccaatg attacggcga
     7441 tgcggtaaaa ggcagcgata aacctgaccg cattgggccg ctacgcggca tgcaaaaagg
     7501 ggtcattacc cagcaagaga tgaaacgggc gctcattatt accgtcgtgc tcatctgtct
     7561 ctccgggttg gcactggttg cagtggcatg ccatacgctg gccgattttg tcggtttcct
     7621 gattcttggc gggttgtcga tcattgccgc tatcacctac accgtgggca atcgtcctta
     7681 tggttatatc ggtctgggtg atatttccgt actggttttc tttggctggt tgagtgtcat
     7741 ggggagctgg tatttacagg ctcatacatt gattccggca ctgatccttc cggcgaccgc
     7801 atgcggcctg ctggcaacgg cagtactgaa tattaataac ctgcgtgata tcaatagcga
     7861 ccgcgaaaat ggcaaaaaca cgctggtggt gcgcttaggt gaagtgaacg cgcgtcgtta
     7921 tcatgcctgc ctgctgatgg gctcgctggt gtgtctggcg ctgtttaatc tcttttcgct
     7981 gcatagcctg tggggctggc tgttcctgct ggcggcacca ttactggtga agcaagcccg
     8041 ttatgtgatg cgggaaatgg acccggtggc gatgcgacca atgctggaac gtactgtcaa
     8101 gggagcgtta ctgactaacc tgctgtttgt tttagggata ttcctaagcc agtgggcagc
     8161 ataactgaca aatatcaatt aacaattgat gattttgcca acagcccaca tagcgcgata
     8221 tactgaaaat tctcgcagca actgaatgtt aagcctatga aatacgatac ttccgagctt
     8281 tgtgacatct atcaagaaga tgttaacgtc gtggaaccgc tgttctccaa ctttggcgga
     8341 cgggcgtcgt ttggcggaca aataatcacg gtaaaatgtt tcgaggacaa cgggttgctg
     8401 tacgatctgc tcgaacagaa tggccgtggt cgtgttcttg tcgtcgatgg cggtggttct
     8461 gttcgtcgcg cactggtcga tgctgaactg gcgcgtctgg cagtacaaaa tgaatgggaa
     8521 ggtctggtca tttacggcgc ggtgcgtcag gtagatgacc tggaagagtt ggatatcggc
     8581 atccaggcga tggcggcaat tccggttggt gccgctggcg aaggcattgg cgaaagcgat
     8641 gtccgcgtca attttggcgg tgtcaccttc ttctccggcg accatcttta tgccgacaat
     8701 accgggatta ttctttctga agatccgctg gatattgaat gatagaaaag gcaccgcaag
     8761 gtgccttttt tctgcgttac ctgttggcct acacagtaaa gaaattacgc ggaagatgaa
     8821 gcgtaatcag acctcttcca tgcgacccag cagggcctgc agacgttcct gccagccgtt
     8881 ctgctgttct ttcagatggt tgttctcacg ctccagctct tcgcgctgat gctgggcatt
     8941 ttgaacttcc tgcgacagtg agttgttttt ttctttcagc tcttcgattt ccatctgcaa
     9001 cagagtgatg gtatcaatcg cctgctgtac ttttgcttcc agtttctcaa acacttctaa
     9061 tgacattgtc atacctctcc tgaattgcaa ggcgttgatg gataaaaatc ctcgtcccga
     9121 ttaccggtga cgccttaata aatacgagcg cactttagtt agctccgatt gtatgaagcc
     9181 gcgccatcgc tgtccagcgg cacgccttgc agattacggt ttgccacact tttcatcctt
     9241 ctcctggtga cataatccac atcaatcgaa aatgttaata aatttgttgc gcgaatgatc
     9301 taacaaacat gcatcatgta caatcagatg gaataaatgg cgcgataacg ctcattttat
     9361 gacgaagcac acacatttta agttcgatat ttctcgcttt tgctcgttaa cgataagttt
     9421 acagcatgcc tacaagcatc gtggaggtcc gtgactttca cgcatacaac aaacattaac
     9481 tcttcaggat ccgattatga gtcaaacatc aaccttgaaa ggccagtgca ttgctgaatt
     9541 cctcggtacc gggttgttga ttttcttcgg tgtgggttgc gttgcagcac taaaagtcgc
     9601 tggtgcgtct tttggtcagt gggaaatcag tgtcatttgg ggactggggg tggcaatggc
     9661 catctacctg accgcagggg tttccggcgc gcatcttaat cccgctgtta ccattgcatt
     9721 gtggctgttt gcctgtttcg acaagcgcaa agttattcct tttatcgttt cacaagttgc
     9781 cggcgctttc tgtgctgcgg ctttagttta cgggctttac tacaatttat ttttcgactt
     9841 cgagcagact catcacattg ttcgcggcag cgttgaaagt gttgatctgg ctggcacttt
     9901 ctctacttac cctaatcctc atatcaattt tgtgcaggct ttcgcagttg agatggtgat
     9961 taccgctatt ctgatggggc tgatcctggc gttaacggat gatggcaacg gtgtaccacg
    10021 cggccctttg gctcccttgc tgattggtct actgattgcg gtcattggcg catctatggg
    10081 cccattgaca ggttttgcca tgaacccagc gcgtgacttc ggtccgaaag tctttgcctg
    10141 gctggcgggc tggggcaatg tcgcctttac cggcggcaga gacattcctt acttcctggt
    10201 gccgcttttc ggccctatcg ttggcgcgat tgtaggtgca tttgcctacc gcaaactgat
    10261 tggtcgccat ttgccttgcg atatctgtgt tgtggaagaa aaggaaacca caactccttc
    10321 agaacaaaaa gcttcgctgt aatatgacta cgggacaatt aaacatgact gaaaaaaaat
    10381 atatcgttgc gctcgaccag ggcaccacca gctcccgcgc ggtcgtaatg gatcacgatg
    10441 ccaatatcat tagcgtgtcg cagcgcgaat ttgagcaaat ctacccaaaa ccaggttggg
    10501 tagaacacga cccaatggaa atctgggcca cccaaagctc cacgctggta gaagtgctgg
    10561 cgaaagccga tatcagttcc gatcaaattg cagctatcgg tattacgaac cagcgtgaaa
    10621 ccactattgt ctgggaaaaa gaaaccggca agcctatcta taacgccatt gtctggcagt
    10681 gccgtcgtac cgcagaaatc tgcgagcatt taaaacgtga cggtttagaa gattatatcc
    10741 gcagcaatac cggtctggtg attgacccgt acttttctgg caccaaagtg aagtggatcc
    10801 tcgaccatgt ggaaggctct cgcgagcgtg cacgtcgtgg tgaattgctg tttggtacgg
    10861 ttgatacgtg gcttatctgg aaaatgactc agggccgtgt ccatgtgacc gattacacca
    10921 acgcctctcg taccatgttg ttcaacatcc ataccctgga ctgggacgac aaaatgctgg
    10981 aagtgctgga tattccgcgc gagatgctgc cagaagtgcg tcgttcttcc gaagtatacg
    11041 gtcagactaa cattggcggc aaaggcggca cgcgtattcc aatctccggg atcgccggtg
    11101 accagcaggc cgcgctgttt ggtcagttgt gcgtgaaaga agggatggcg aagaacacct
    11161 atggcactgg ctgctttatg ctgatgaaca ctggcgagaa agcggtgaaa tcagaaaacg
    11221 gcctgctgac caccatcgcc tgcggcccga ctggcgaagt gaactatgcg ttggaaggtg
    11281 cggtgtttat ggcaggcgca tccattcagt ggctgcgcga tgagatgaag ttgattaacg
    11341 acgcctacga ttccgaatat ttcgccacca aagtgcaaaa caccaatggt gtgtatgtgg
    11401 ttccggcatt taccgggctg ggtgcgccgt actgggaccc gtatgcgcgc ggggcgattt
    11461 tcggtctgac tcgtggggtg aacgctaacc acattatacg cgcgacgctg gagtctattg
    11521 cttatcagac gcgtgacgtg ctggaagcga tgcaggccga ctctggtatc cgtctgcacg
    11581 ccctgcgcgt ggatggtggc gcagtagcaa acaatttcct gatgcagttc cagtccgata
    11641 ttctcggcac ccgcgttgag cgcccggaag tgcgcgaagt caccgcattg ggtgcggcct
    11701 atctcgcagg cctggcggtt ggcttctggc agaacctcga cgagctgcaa gagaaagcgg
    11761 tgattgagcg cgagttccgt ccaggcatcg aaaccactga gcgtaattac cgttacgcag
    11821 gctggaaaaa agcggttaaa cgcgcgatgg cgtgggaaga acacgacgaa taatgtaaat
    11881 gccgaatgaa gcgtttatgc cgcatccggt agtcccgaaa cgtgcggggg caaccccgca
    11941 cacatcaata atccctccct tcccctgtgc tacacttcgc gccattcctt actgcttaga
    12001 gtttgctatg agacgagaac ttgccatcga attttcccgc gtcaccgaat cagcggcgct
    12061 ggctggctac aaatggttag gacgcggcga taaaaacacc gcggacggcg cggcggtaaa
    12121 cgccatgcgt attatgctca accaggtcaa cattgacggc accatcgtca ttggtgaagg
    12181 tgaaatcgac gaagcaccga tgctctacat tggtgaaaaa gtcggtactg gtcgcggcga
    12241 cgcggtagat attgctgttg atccgattga aggcacgcgc atgacggcga tgggccaggc
    12301 taacgcgctg gcggtgctgg cagtaggcga taaaggctgc ttcctcaatg cgccggatat
    12361 gtatatggag aagctgattg tcgggccggg agccaaaggc accattgatc tgaacctgcc
    12421 gctggcggat aacctgcgca atgtagcggc ggcgctcggc aaaccgttga gcgaactgac
    12481 ggtaacgatt ctggctaaac cacgccacga tgccgttatc gctgaaatgc agcaactcgg
    12541 cgtacgcgta tttgctattc cggatggcga cgttgcggcc tcaattctca cctgtatgcc
    12601 agacagcgaa gttgacgtgc tgtacggtat tggtggcgcg ccggaaggcg tagtttctgc
    12661 ggcggtgatc cgcgcattag atggcgacat gaacggtcgt ctgctggcgc gtcatgacgt
    12721 caaaggcgac aacgaagaga atcgtcgcat tggcgagcag gagctggcac gctgcaaagc
    12781 gatgggcatc gaagccggta aagtattgcg cctgggcgat atggcgcgca gcgataacgt
    12841 catcttctct gccaccggta ttaccaaagg cgatctgctg gaaggcatta gccgcaaagg
    12901 caatatcgcg actaccgaaa cgctgctgat ccgcggcaag tcacgcacca ttcgccgcat
    12961 tcagtccatc cactatctgg atcgcaaaga cccggaaatg caggtgcaca tcctctgatt
    13021 gatttgatcg attgagcctt ccagtccttc gggactggaa tttttttgtt cggagaacga
    13081 agataaggca agtcaatcaa aacaggagaa aaacatggct gattgggtaa caggcaaagt
    13141 cactaaagtg cagaactgga ccgacgccct gtttagtctc accgttcacg cccccgtgct
    13201 tccgtttacc gccgggcaat tcaccaagct tggccttgaa atcgacggcg aacgcgtcca
    13261 gcgcgcctac tcctatgtta actcgcccga taatcccgat ctggagtttt acctggtcac
    13321 cgtccccgat ggcaaattaa gcccacgatt ggcggcactg aaaccaggcg atgaagtgca
    13381 ggtggttagc gaagcagctg gcttctttgt tctggatgaa gtaccggact gcgaaacgct
    13441 atggatgctg gcaaccggta cagcgattgg cccttattta tcgattctgc aactaggcaa
    13501 agatttagat cgcttcaaaa atctggtcct ggttcacgcc gcacgttatg ccgccgactt
    13561 aagctatttg ccactgatgc aagaactgga aaaacgctac gaagggaaac tgcgcattca
    13621 gacggtggtc agtcgggaaa cggcagcggg gtcgctcacc gggcggatac cggcgttaat
    13681 tgaaagtggg gaactggaaa gcgcgattgg cctgccgatg aataaagaaa ccagccatgt
    13741 gatgctgtgc ggcaatccac agatggtgcg cgatacacaa cagttgctga aagagacccg
    13801 gcagatgacg aaacatttac gtcgccgacc gggccatatg acagcggagc attactggta
    13861 agcggttact tatcgataaa cggcacgatg agcaaatccg cactcatctt attgatcatc
    13921 ccgcgatatg ccggcatcaa acggttgata aatgagtgat gatgaccaca gacaaggagg
    13981 tcgcactgct ctttttgcat aatttccagc agtgtttccg gcatttctcc gcgttcaata
    14041 cgcagttttg tcttcggcca ttgaatattt ttcgtcagtt tatacagctt gttatccgac
    14101 ttattcttca acaattgaag aatatcttct gttgcaggga agtagatacc cggatacaac
    14161 tcgcttaagc catcatcaat atgaattaac gtcaggtgag cgtcattatg tctggcgagc
    14221 tccagagctt tattcaccag taaggcatct tcttcattcc cggaaattgc cacgccaatg
    14281 tgtttataag ccatgtttaa ctccttctaa agcaactcca tcaagctaac gaacgcagtg
    14341 ataactcaaa aataatcatc tcggcctggc atgagaaagt gaaggccgca tcaagctcaa
    14401 cttgcccctc cagatcctta atcttgtagg ccacgctggc cccttcatct gcagaggatg
    14461 cagcggcggc aagctctttc agtttggtca taaaagactc tgcttcttcg cgggtggcaa
    14521 aaaagcgcga aaacttactg gtacagttgt cgttatcaat caccgtaccg atatcaatcg
    14581 cacatccttt agtactgcat ttatctacga catctttcat agggcacctc tgtatattcg
    14641 cctctctgtc gattacgact taagaataat gcgccgttaa gggaagaaca aacaagtaat
    14701 gatcttatgg attaccattt tttttgatat tgcttaaggt gaagagttca gaaggatttt
    14761 ccgggtattg tcatttgcat cgtggaaata acaatcccgg aaaaaaatag cagtaagaga
    14821 tatattaaag gttaattact tgatttattg tcggctttat acttcacatc ctgagtatct
    14881 ttaccatatt tattttcgcc ttgtgtgcca acaaacgcac caagatcgat aagcatcatc
    14941 accagaatca acgtcgggac aaaacgcccc accgcccatt gccagacacc cggtaaaatc
    15001 gcccagttac ccgccagcag catccacgcc acaatcatca gaaatgccca tgcgccggaa
    15061 cgcccgcgat catgcaagcg cttaacagtt actgccgctg ttggccaaag caagcacaca
    15121 aggcaaaacg ccgcggtctg aatatcgagt aaatttttac ccgccagtga gaaaagcacc
    15181 agcatgcctg cgaaccacag gcctatccaa atccagaaat cacggcgtcc aatacgccct
    15241 ttaaatgaga ataaccattg ctgtatggtc atgtaagttc cttgatggtt gtcttttcca
    15301 ggattctacc cttttgacaa gggcgacaga tatcgtttta atcggagcca gtcataacaa
    15361 aaggtactgt caatgaagcc agggtgtacg ctgttttttc tcttatgttc tgcattaacc
    15421 gttacaacaa cggcgcatgc acaaacacca gatacggcaa cgaccgcgcc ttatctgctg
    15481 gctggagccc ctactttcga tctctccatc agccagtttc gagaagactt taacagccag
    15541 aatcctagcc tgccactgaa cgaatttcgt gccatcgaca gcagtcccga caaagccaat
    15601 ctcactcgtg ctgcaagtaa aattaacgag aacttgtatg cttctacagc gctggagcgc
    15661 ggtaccttaa aaatcaaaag cattcaaatg acctggctac ccatccaggg gccagagcaa
    15721 aaagcagcga aagcgaaagc tcaggaatac atggcagcag tgatccgcac actcacccca
    15781 ttaatgacca aaacacaaag ccagaaaaaa ctgcagtcgc tactaacggc ggggaaaaac
    15841 aaacgttatt acaccgagac agaaggtgca ctgcgttatg ttgtcgcgga caacggcgaa
    15901 aaggggctga ccttcgctgt tgaaccgatt aagctggcgc tatctgaatc gcttgaaggt
    15961 ttgaataaat gacaaaaagc aaagcctttg tgccgatgaa tctctatact gtttcacaga
    16021 cctgctgccc tgcggggcgg ccatcttcct ttattcgctt ataagcgtgg agaattaaaa
    16081 tgcgacatcc tttagtgatg ggtaactgga aactgaacgg cagccgccac atggttcacg
    16141 agctggtttc taacctgcgt aaagagctgg caggtgttgc tggctgtgcg gttgcaatcg
    16201 caccaccgga aatgtacatc gatatggcga agcgcgaagc tgaaggcagc cacatcatgc
    16261 tgggtgcgca aaacgtggac ctgaacctgt ccggcgcatt caccggtgaa acttctgccg
    16321 ctatgctgaa agacatcggc gctcagtaca tcatcatcgg ccactctgaa cgtcgtactt
    16381 accacaaaga gtctgacgaa ctgattgcga aaaaattcgc ggtgctgaaa gagcagggcc
    16441 tgactccggt tctgtgcatc ggtgaaaccg aagctgaaaa cgaagcgggc aaaactgaag
    16501 aagtttgcgc acgtcagatc gacgcggtac tgaaaactca gggtgctgcg gcattcgaag
    16561 gtgcggttat cgcttacgaa cctgtatggg caatcggtac tggcaaatct gcaactccgg
    16621 ctcaggcaca ggctgttcac aaattcatcc gtgaccacat cgctaaagtt gacgctaaca
    16681 tcgctgaaca agtgatcatt cagtacggcg gctctgtaaa cgcgtctaac gctgcagaac
    16741 tgtttgctca gccagatatc gacggcgcgc tggttggcgg tgcttctctg aaagctgacg
    16801 ctttcgcagt gatcgttaaa gctgcagaag cggctaaaca ggcttaagtc tgacaggtgc
    16861 cggatttcat atccggcact tactttcctt aactcctcgc cttaacgcaa aatctcacac
    16921 tcatgatcct ggatttcctc ggctgaagca cggttcagcg tcagtagatt tcgttgtgtc
    16981 gccagcaaca caaacgaatt atcactctgc cgtaccatcg ccagcccgta gcttcccata
    17041 tgttcccgcg cctcaggcac ttcttctgca agcatcataa atgggctgcg ttgtaccagt
    17101 tcgctttccg ttacccgacg cgccaggtat tcatgcccgc gcaaaccacc tggcagtggc
    17161 aaccagcggc tgctgatgtt cgccagattt ttatccagtt gttcgcgcac atcaggacga
    17221 atacaagaga tatgaatatg aaaatggttt tgcgtacgcc cggtgcggga gttgatcgcc
    17281 aaagaaaccg cgcgatcggg aaccggctgg ccgtattttt tgctcataaa atcacgcgcc
    17341 tgccaggcca accaaaagaa gttcggcgtt gaaggatcgg tcaacaaagg actttcagta
    17401 ccgttaatac gatacgttgg catcaacaga tattgcagtg gaccattaag atcttttaaa
    17461 accacgtatc cggcattggg tttgacttcc gcacatggcg aaggattttg attttgctgc
    17521 tgattgggca aacattcctc aaggacaatc ttacgtaatg tatccgactc ttcaccggtt
    17581 aatttccagt aaccaatacc ggcagccaca acggcgataa ctatcatcac caaaaaaaga
    17641 agacccgctt ttttcatctt tttttccctg tacctcaaag agatgcaagg gtaacgcaaa
    17701 atcgtgacaa ataaaaaacc cggtcgcgag aaaccaccgg gtttgataat tatcctgggg
    17761 aaatcagcgt ttgctgatct gatcgaacgt accgccgtta gcaaaatgct ctttttgcgc
    17821 tttcgtccag ccgccgaact cttcatcaat ggtgaataac ttcagctttg gaaacgcatt
    17881 ttcgtacttt ttcgctactt cagcgtcgcg cggacggtag tagtttttcg cggcaatttc
    17941 ctgaccttct ggcgagtaga gatatttcag gtaggcttcc gccacctctt tggtgccttt
    18001 tttctcgacc actttatcga ccaccgacac ggttggctct gcgaggatag actcactcgg
    18061 cgtgacgatt tcgaatttat ctttccccag ttcattcgct accagcagag cttcgttttc
    18121 ccaggcgatc agtacatcgc caatcccgcg ctcgacaaag gtgttagtgg agccgcgcgc
    18181 gccagaatcc agaacttcga cgtttttata cagtgcccga acaaaatcct gtgcttttgc
    18241 ctgatcgttg ttgttgtgat gcagcgcgta gccccaggca gccaggtagt tccagcgtgc
    18301 gccaccagag cttttcggat taggcgtgat caccgaaaca cccggtttaa tcagatcgtt
    18361 ccagtcatgg atctgcttcg gattgccctt acgcaccagg aaaacaatgg tggaagtgta
    18421 cggtgcggag ttatccggca gacgtttgat ccactcttta tcaatccgcc cgcgttccgc
    18481 aatcgcgtcc acgtcatagg ccagagccag cgtgacaaca tcagcttcaa taccgttgat
    18541 taccgacgtc gcttgtttac ctgaaccacc gtgtgactga cgaatcacca cgttatcacc
    18601 agtttgctgt ttccagtggg cgctgaatgc cttgttgtac tgttcgtaca attcgcgcgt
    18661 tggatcatat gaaacgttaa gaagctgaat atcctttgcc ataacgttgg ttgccgccag
    18721 caaaaatgtt aaccctacgc cccacttgtt catcgcccga ctctcttatg ttgtgttgtg
    18781 atgagcaaag cgtgccagaa ggttaaccaa acattaaaga ataaaaaaag attggctata
    18841 acttgcgggt atatgttggg ggttaaaaag gcggaagaaa tccgcctcat attgctgaca
    18901 aagtgcgctt tgtccatgcc ggatgcggcg cgaacgcctt atccggccta caaaagtttg
    18961 caaattcaat aaattgcaga attcatgtag gcctgataag cgaagcgcat caggcatttt
    19021 tgcttctgtc atcggtttca gggtaaagga atctgccttt ttccgaaatc attaatacag
    19081 ttttttcgcg cagtccagcc agtcaccttt gaacggacgc ttcatgtttt cgatagcgtc
    19141 gatgatgtcg tggtgaacca gctgttcgtt ctggatacct acacaacgac cgccgtaacc
    19201 tgccagcagc agatcgatag cgtaagcgcc catacgggaa gccagaatac ggtcgtaagg
    19261 caccggagaa ccaccgcgct ggatgtggcc cagcacagtt gcgcgggttt cacgaccggt
    19321 ttctttctcg atgaaatgcg ccagttcgtc aacatcacac atatgttcgg taatcgccac
    19381 gatcgcgtgt tttttacctt tcgcgatacc cgctttgatt tcgtttacca ggtcttcacg
    19441 gctgaattca acttccggaa ccacaacgaa ttcacagcca ccggcaatgg ccgcagccaa
    19501 cgtcagatcg ccacaataac ggcccatcac ttccaccacg gaaatacgct ggtgagaaga
    19561 agaggtgtca cgcagacggt cgatcgcttc tacaacggtg ctcagcgcag tgaagaaacc
    19621 gatagtgtag tcagtgcctt tgatgtcgtt gtcgatagtg cccggcagac cgatgcacgg
    19681 gaagcccatt tcggtcagac gcattgcacc catgtaggaa ccgtcaccgc cgataaccac
    19741 cagcgcgtcg atcccacgtt ttttcaggtt ttcgatagcc acggcgcgga tgttctcgtc
    19801 gcggaattcc gggaagcgcg cagaaccgag gaacgtaccg ccacggttga tcatgtcaga
    19861 aacgctgtaa cggtctagct gtaccatacg gtcttcatac agacccagat agccgtcata
    19921 aatgcccatt acttccagac cttctgtcag cgcagaacga acaaccccgc gaattgcggc
    19981 gttcatgcct ggcgcatcac cgccgcttgt caacacaccg attttcttaa tcatgactac
    20041 ctctgaactt tggaatgcaa aataaaatct gttgccggaa gtcttcttgc acatcgaagt
    20101 gatccaacga atgtgcaaat agtataacaa tcacttcctg ctgaattgat tcaggtcagg
    20161 ccaaatggcg gtattttata cacaaaatgc gggtctggct ctcttttata ctgattatga
    20221 aagcatagac cgtttaccct ccctgggtac gacggaacag gggtcctgat ggataattac
    20281 atccgatccc ggaaaacgcc gtaaaatagc ctgctctacc tgatccgcca ccatatgtgc
    20341 ctgaaccaaa ggcagagagt cttccatttc caaatgaatc tgaataaagc gggtcggccc
    20401 tgactgccgc gtgcgaagat cgtgagcgcc gctaacaccc ggccaggaag tcacgatatc
    20461 aataatttct tgccgttcct catcaggcaa tgcgcgatcc agtaatgact gtaccgcctc
    20521 atatcccatg cgtaacgcgc tatataaaat atagatgccg attcccaatg caaacagagc
    20581 atcggcgcga tgccagccgt accaggacaa ccccagcgcc agcagaattg cgccgttcat
    20641 cataacatca gactggtaat gtagcatatc agcccgcacc gcctggcttt gcgtccggcg
    20701 caccacccaa cgctgaaacg agacaaggat aatcgtacaa attagcgcca caattgtcac
    20761 gataaccccg acgcctggat ctgtcatcgg tgttggagat atcagatgtt gaatacccgt
    20821 caaaaacagg aatagtgccg aaccggagat aaacatactt tgcgccagcg ccgcgaggga
    20881 ctctgcttta ccgtgaccaa acgagtgatt atcgtcggca ggttgcaggg aatatcgcac
    20941 caccagtaaa ttcgtcaacg acgcgccgat atccaccagc gaatccacca gcgcggcgag
    21001 aatactcacc gacccggtat accaccatgc aaaaatttta atcagcaata gcaacgaagc
    21061 catcgccgtc gcagcaatcg ccgcccgact gaccagccgt ccataagatt gattcataaa
    21121 tactcccgct atcaactgac gctagtataa cggaagcaaa tcatctgcaa tgcattaagc
    21181 agcaggcaaa ttgaggataa aaaaaacccc cacatcatgt gggggaagac agggatggtg
    21241 tctatggcaa ggaaaacagg gtttactact gggaacgtga gttgctacta ctcaatagct
    21301 tcaacgatga acttttttgc cattgcgtca cgtcacgcaa ctgctccatt cgttgttgat
    21361 gtttctcgtt taaaaccgct tgctgctccg gcgttaacag gcgatacatt tggttgcgga
    21421 cttttgccat ctcaacctga cgagcaattt gctcattcgc cattttttct gcctgtgcgc
    21481 gcacagcgtt ttcatcaaaa ttttctgcgg tgacaaggcg atgcattgtc tccagttcgc
    21541 taacattaac aggaggctgt tcgtgccggg cctgttgcat aagatctcgc atctgctgac
    21601 gctgatgttc ggttaaactt atgccgtcga acatatggct ctgcgtactg cgctgcgtaa
    21661 gttcttcacc cggatgccag ttatcgcctg aaccgacttc agcagcgtgg cttaatgaac
    21721 tgactgccag cgttgaggcc atgacggcag cggtaactat gcgcatcatt tgctcccaaa
    21781 atctttctgt cgcgattcaa cgatagagag tttacgattc aggctgcaaa catgcgtcag
    21841 ggggtgtaaa acaacgtaaa gtcatggatt agcgacgtct gatgacgtaa tttctgcctc
    21901 ggaggtattt aaacaatgaa taaaatcctg ttagttgatg atgaccgaga gctgacttcc
    21961 ctattaaagg agctgctcga gatggaaggc ttcaacgtga ttgttgccca cgatggggaa
    22021 caggcgcttg atcttctgga cgacagcatt gatttacttt tgcttgacgt aatgatgccg
    22081 aagaaaaatg gtatcgacac attaaaagca cttcgccaga cacaccagac gcctgtcatt
    22141 atgttgacgg cgcgcggcag tgaacttgat cgcgttctcg gccttgagct gggcgcagat
    22201 gactatctcc cgaaaccgtt taatgatcgt gagctggtgg cacgtattcg cgcgatcctg
    22261 cgccgttcgc actggagcga gcaacagcaa aacaacgaca acggttcacc gacactggaa
    22321 gttgatgcct tagtgctgaa tccaggccgt caggaagcca gcttcgacgg gcaaacgctg
    22381 gagttaaccg gtactgagtt taccctgctc tatttgctgg cacagcatct gggtcaggtg
    22441 gtttcccgtg aacatttaag ccaggaagtg ttgggcaaac gcctgacgcc tttcgaccgc
    22501 gctattgata tgcacatttc caacctgcgt cgtaaactgc cggatcgtaa agatggtcac
    22561 ccgtggttta aaaccttgcg tggtcgcggc tatctgatgg tttctgcttc atgataggca
    22621 gcttaaccgc gcgcatcttc gccatcttct ggctgacgct ggcgctggtg ttgatgttgg
    22681 ttttgatgtt acccaagctc gattcacgcc agatgaccga gcttctggat agcgaacagc
    22741 gtcaggggct gatgattgag cagcatgtcg aagcggagct ggcgaacgat ccgcccaacg
    22801 atttaatgtg gtggcggcgt ctgttccggg cgattgataa gtgggcaccg ccaggacagc
    22861 gtttgttatt ggtgaccacc gaaggccgcg tgatcggcgc tgaacgcagc gaaatgcaga
    22921 tcattcgtaa ctttattggt caggccgata acgccgatca tccgcagaag aaaaagtatg
    22981 gccgcgtgga actggtcggt ccgttctccg tgcgtgatgg cgaagataat taccaacttt
    23041 atctgattcg tccggccagc agttctcaat ccgatttcat taacttactg tttgaccgcc
    23101 cgctattact gctgattgtc accatgttgg tcagtacgcc gctgctgttg tggttggcct
    23161 ggagtctggc aaaaccggcg cgtaagctga aaaacgctgc cgatgaagtt gcccagggaa
    23221 acttacgcca gcacccggaa ctggaagcgg ggccacagga attccttgcc gcaggtgcca
    23281 gttttaacca gatggtcacc gcgctggagc gcatgatgac ctctcagcag cgtctgcttt
    23341 ctgatatctc tcacgagctg cgcaccccgc tgacgcgtct gcaactgggt acggcgttac
    23401 tgcgccgtcg tagcggtgaa agcaaggaac tcgagcgtat tgaaaccgaa gcgcaacgtc
    23461 tggacagcat gatcaacgat ctgttggtga tgtcacgtaa tcagcaaaaa aacgcgctgg
    23521 ttagcgaaac catcaaagcc aaccagttgt ggagtgaagt gctggataac gcggcgttcg
    23581 aagccgagca aatgggcaag tcgttgacag ttaacttccc gcctgggccg tggccgctgt
    23641 acggcaatcc gaacgccctg gaaagtgcgc tggaaaacat tgttcgtaat gctctgcgtt
    23701 attcccatac gaagattgaa gtgggctttg cggtagataa agacggtatc accattacgg
    23761 tggacgacga tggtcctggc gttagcccgg aagatcgcga acagattttc cgtccgttct
    23821 atcggaccga tgaagcacgc gatcgtgaat ctggcggtac aggattgggg ctggcgattg
    23881 ttgaaaccgc cattcagcag catcgtggct gggtgaaggc agaagacagc ccgctgggcg
    23941 gtttacggct ggtgatttgg ttgccgctgt ataagcggag ttaaactccg catttgtagg
    24001 caggataagg cgtttacgcc gcatccggca tttgagcagg ataaggcgtt tacgccgcat
    24061 ccggcatttg agcaggatgc ctgatgcgac gctgatagcg tcttatcagg cctacactcc
    24121 gggggttatt ttccccacaa ccggcgggaa aaatcttcga tcttgccgct cagtcggcgc
    24181 ttttgcatcg tccgcgtcca gcttttcgat aacccggcgg cggagagtaa acggtgatac
    24241 tgatcgtcat caaacggcat atgccatgcg atggcggcag cctcctgcac ggtcacatcg
    24301 ctgacacggg aaaccaactc cagcggcgcg tccgcagaca ctttccctgg cgctatcacg
    24361 ctatacaacc accccacctt acctgtgttt tgcatcaact gcgctatatc gctgatatca
    24421 aaatggtaat tgagtttata gcaaggcgag cgcggctggc tgacctgaat taatgcctct
    24481 ccccagcgga aaatatcgcc catatgaaca ttactttccg tgagaccgtc ggttgagaga
    24541 ttttcgccaa acgcaggcgc aacaaacaac tccgcctgtt ccggaaattc ccgcacccag
    24601 tagagatagt gctcacgagg ataatgacac agcgctctgt ctggcccgcc gtgaaccttt
    24661 ttctccgcct gctcgtcacc ttccagcccc agctccgtca gcatcaactc accatcaacc
    24721 tggattttgg caattgcgct gggtttgcct tcgggataag cctgaatctt gcctgtgtat
    24781 acatcaaccg gatatcgcat ctgttccctc tccacgagaa atttccccat tagaccatat
    24841 ttattttatg tcaggcgtgc tgttttcgcg gtaagtatcc aggcaggaac tcaatagcaa
    24901 accggacgaa cgttagttca tccggctgtg gggattaagg aagcaggttt atttcaccgt
    24961 acctaaaatt tcgattttcg ctgctctggc tttgacttta cgtgaccaga tagaggtaag
    25021 aattggcacc agaatcgaag tcacaatgac cgccgtcgct accagcgaag ttgctgccgg
    25081 agccatcggt ttaaacgcag gcaccatttc tgcaatcagc acaggtgtcg ctaccgcggc
    25141 ccctgcggaa ctggaagcgg caattccggc agtgccatcg ccaccgccaa tcaatttatc
    25201 ggcgataatc aacggaatac cggtcacgat aattactgcc acacccaaca ggatccccag
    25261 caaaccagtc tgggcaatca cagtcaaatc aatggtattg cccagcgcga aggcaaagaa
    25321 tggaatcagc gtttgcaccg ctttgctgaa aaattctcgt aattcagggt caaggttccc
    25381 cagggcaaag cccaccagga acggtaatac tgcgccgacg aaaacatgcg gttcaaacga
    25441 ggcaatccct gcagtgccca gaataatcat cgtcatgagc ggcccggact ccaacgacat
    25501 caacacaaat gccccagctt cttcttttgt gccgtactgc tgcatgatgg aagcgtaaag
    25561 tccgccattg gtcatatcca tcgccgccac cagcgccagc gttgaaagtc cggcaaagaa
    25621 tccaacttca acaccatgtt ccggaataat gcgcgaggca attgccgcaa ccacccacgc
    25681 gacggcaatt tttgttacca ccagcgtacc ggatttacgc aataccgttc ccgtcgcgct
    25741 taattttatt gacgccccca tgcaaaaaaa ccacaccgcc agaatgggca ccgtaccggt
    25801 aatcatcccg ttggtgaatg atccaaaata tttccccgcc cccggcgaga aggtgtggca
    25861 cagtgcgcca aggaatagcg ggacgagcat catccccccc gggattttct caatcgagcg
    25921 ttttatctgc atttccatca cctgtcggca tatttttaag ggctgatgcg tcattcctca
    25981 atggagcatc agtgttgatg gaaacgaaga tagacattca tttaatcaat aaaagtgatc
    26041 aaaaccacat caattgaaac gctgttttat ttttatcgga tcattgtttt tattttgaaa
    26101 ttgatcacaa aaaaaccacc gccgttggcg atggttcatt ttttaagctg atatgcggcc
    26161 tatacgcctc attgcagcag gcggcaaatg attatttttt cgccgcaaaa cgtgccgctg
    26221 cttcgtccca gttcaccacg ttccagaact ctttaatgta gtccggacgg cggttctgga
    26281 atttcaggta gtaagcatgt tcccacacat ccaggcccag aatcgggaag ccggaagcgc
    26341 cagaaatagc ttcacccatc agcggagaat cctggttagc agtagaaacc accgccagtt
    26401 tatcgccttt cagcaccagc catgcccagc cggaaccaaa gcgggaagct gccgcttttt
    26461 caaattctgc tttgaagtta tcaacggagc cgaagtcacg ttcgatagcc gctttcaggt
    26521 caccctgcag ggtggtgcct tttttcagac ctttccagaa caggctgtgg ttagcgtgac
    26581 cgccagcgtt gttgcgcagt acggttttct tgtctgctgg cagctggtcc agtttggtga
    26641 tcagctcttc aaccggcagg ttggcaaatt ctggcaggct ttccagcgcc gcgttggcgt
    26701 tgtttacgta ggtctgatgg tgtttggtgt ggtggatttc catggtctgc ttatcgaagt
    26761 gcggttccag ggcatcgtaa gcatacggca gggatggcag ggtatagctc atattcatct
    26821 ccagtattgt cgggcggccg attgttaatg ccgcgtaagc agttggttca ttatagttaa
    26881 ttaaatgata ttgaaaatga ttatcaatgc cgtacttttc gtaagggtat ggttttgcag
    26941 gaaaatgccc gagatgtgaa gcaaatcacc cacttaatgc cgtgattgcc agtaaatcga
    27001 caacggcggc aacaggcgaa aggttaatcg acagcacgat ttttacactc atctcgtcgg
    27061 agatgtgaag cgacgaaaaa tgatgaggat aagaagatga gtaacgcgat tacgatgggg
    27121 atattttggc atttgatcgg cgcggccagt gcagcctgtt tttacgctcc gttcaaaaaa
    27181 gtaaaaaaat ggtcatggga aaccatgtgg tcagtcggtg ggattgtttc gtggattatt
    27241 ctgccgtggg ccatcagcgc cctgttacta ccggatttct gggcgtatta cagctcgttt
    27301 agtctctcta cgctgctgcc tgtttttctg ttcggcgcta tgtgggggat cggtaatatc
    27361 aactacggcc tgaccatgcg ttatctcggc atgtcgatgg gaattggcat cgccattggc
    27421 attacgttga ttgtcggtac actgatgacg ccaattatca acggcaattt cgatgtgctg
    27481 attaacaccg aaggcggacg catgacgttg ctcggcgttc tggtggcgct gattggcgta
    27541 gggattgtaa ctcgagccgg gcagttgaaa gagcgcaaga tgggcattaa agccgaagag
    27601 ttcaatctga aaaaagggct ggtgctggcg gttatgtgcg gcattttctc tgccgggatg
    27661 tcctttgcga tgaacgccgc aaaaccgatg catgaagccg ctgccgcact tggcgtcgat
    27721 ccactgtatg tcgctctgcc aagctatgtt gtcatcatgg gcggcggcgc gatcattaac
    27781 ctcggtttct gttttattcg tctggcaaaa gtgaaggatt tgtcgctaaa agccgacttc
    27841 tcgctggcaa aaccgctgat cattcacaat gtgttactct cggcactggg cgggttgatg
    27901 tggtatctgc aattcttttt ctatgcctgg ggccacgccc gcattccggc gcagtatgac
    27961 tacatcagtt ggatgctgca tatgagtttc tatgtattgt gcggcggtat cgtcggactg
    28021 gtgctgaaag agtggagcaa tgcaggacgc cgtccggtaa cggtgttgag cctcggttgt
    28081 gtggtgatta ttgtcgccgc taacatcgtc ggcatcggca tggcgaatta atctttctgc
    28141 gaattgagat gacgccactg gctgggcgtc atcccggttt cccgggtaaa caccaccgaa
    28201 aaatagttac tatcttcaaa gccacattcg gtcgaaatat cactgattaa caggcggcta
    28261 tgctggagaa gatattgcgc atgacacact ctgacctgtc gcagatattg attgatggtc
    28321 attccagtct gctggcgaaa ttgctgacgc aaaacgcgct cactgcacga tgcctcatca
    28381 caaaatttat ccagcgcaaa gggacttttc aggctagccg ccagccgggt aatcagctta
    28441 tccagcaacg tttcgctgga tgttggcggc aacgaatcac tggtgtaacg atggcgattc
    28501 agcaacatcg ccaactgccc gaacagcaac tctgccattt cgttagcaaa cggcacatgc
    28561 tgactacttt catgctcaag ctgaccgata acctgccgcg cctgcgccat ccccacgcta
    28621 cctaagcgcc agtgtggttg ccctgcgctg gcgctaaatc ccggaatcgc cccctgccag
    28681 tcaagattca gcttcagccg ctccgggcaa taaataatat tctgcaaaac cagatcgtta
    28741 acggaagcgt aggagtgttt atcgtcagca tgaatgtaaa agagatcgcc acgggtaatg
    28801 cgataagggc gatcgttgag tacatgcagg ccattaccgc gccagacaat caccagctca
    28861 caaaaatcat gtgtatgttc agcaaagaca tgttgcggat aacggtcagc cacagcgact
    28921 gcctgctggt cgctggcaaa aaaatcatct ttgagaagtt ttaactgatg cgccaccgtg
    28981 gctacctcgg ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa tacgttgaga
    29041 agattcgcgt tattgcagaa aaccatcccg tccctgacga atatcacgcg gtgaccagtt
    29101 aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccgc aatgataggc
    29161 gatgtcagta acgctggcct cactgtggcg tagcagatgt cgggccttca tcagtcgcag
    29221 gcggttcagg taacgctgag gcgtcagtcc cgtttgctgc ttaagctgcc gatgtagcgt
    29281 tcgcagtgaa agagaaaatt gatccgccac ggcatcccaa ttcacctcat cggcaaaatg
    29341 gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca ggttcttctg
    29401 caaactgctt ttacgcagca agagcagtaa ttgcataaac aaagatctcg cgactggcgg
    29461 tcgagggtaa atcattttcc ccttcctgct gttccatctg tgcaaccagc tgtcgcacct
    29521 gctgcaatac gctgtggtta acgcgccagt gagacggata ctgcccatcc agctcttgtg
    29581 gcagcaactg attcagcccg gcgagaaact gaaatcgatc cggcgagcga tacagcacat
    29641 tggtcagaca cagattatcg gtatgttcat acagatgccg atcatgatcg cgtacgaaac
    29701 agaccgtgcc accggtgatg gtatagggct gcccattaaa cacatgaata cccgtgccat
    29761 gttcgacaat cacaatttca tgaaaatcat gatgatgttc aggaaaatcc gcctgaggga
    29821 gccggggttc tatcgccacg gacgcgttac cagacggaaa aaaatccaca ctatgtaata
    29881 cggtcatact ggcctcctga tgtcgtcaac acggcgaaat agtaatcacg aggtcaggtt
    29941 cttaccttaa attttcgacg gaaaaccacg taaaaaacgt cgatttttca agatacagcg
    30001 tgaattttca ggaaatgcgg tgagcatcac atcaccacaa tgaagcaaat tgtgaacatc
    30061 atcacgttca tctttccctg gttgccaatg gcccattttc ctgtcagtaa cgagaaggtc
    30121 acgaattcag gcgcttttta gactggtcgt aatgaaattc agcaggatca cattatgacc
    30181 tttcgcaatt gtgtcgccgt caatctcggc gcatccagtg ggcgcgtgat gctggcgcgt
    30241 tacgagcgtg aatgccgcag cctgacgctg cgcgaaatcc atcgttttaa aaatgggctg
    30301 catagccaga acggttatgt cacctgggat gtggatagcc tggaaagtgc cattcgcctt
    30361 ggcttaaaca aagtgtgtga ggaagggatt cgtatcgata gcattgggat tgatacctgg
    30421 ggcgtggact ttgtgctgct cgaccaacag ggtcagcgtg tgggcctgcc cgttgcttat
    30481 cgcgatagcc gcaccaatgg cctaatggcg caggcacagc aacaactcgg caaacgcgat
    30541 atttatcaac gtagcggcat ccagtttctg cccttcaata cgctttatca gttgcgtgcg
    30601 ctgacggagc aacaacctga acttattcca cacattgctc acgctctgct gatgccggat
    30661 tacttcagtt atcgcctgac cggcaagatg aactgggagt acaccaatgc caccaccaca
    30721 caactggtca atatcaatag cgacgactgg gacgagtcgc tactggcgtg gagcggggcc
    30781 aacaaagcct ggtttggtcg cccgacgcat ccgggtaatg tcattgggca ctggatttgc
    30841 ccgcagggta atgagattcc ggtggtcgcc gttgccagcc atgataccgc cagcgcggtt
    30901 atcgcctcgc cgttaaacgg ttcacgcgcc gcttatctct cttctggcac ctggtcattg
    30961 atgggcttcg aaagccagac gccatttacc aatgacacgg cgctggcagc caacatcacc
    31021 aatgaaggcg gggcggaagg tcgctatcgg gtgctgaaaa atattatggg cttatggctg
    31081 cttcagcgag tgcttcagga acggcaaatc aacgatctcc cggcgcttat cgccgcgaca
    31141 caggcacttc cggcctgccg cttcatcatc aatcccaatg acgatcgctt tattaatcct
    31201 gaggcgatgt gcagcgaaat tcaggctgcg tgtcgggaaa cggcgcaacc gatcccggaa
    31261 agtgatgctg aactggcgcg ctgtattttc gacagtctgg cgctgctgta tgccgatgtg
    31321 ttgcatgagc tggcgcagct gcgcggtgaa gatttctcgc aactgcatat tgtcggcggc
    31381 ggctgccaga acacgctgct caaccagcta tgcgccgatg cctgcggtat tcgggtgatc
    31441 gccgggcctg ttgaagcctc aacgctcggc aatatcggca tccagttaat gacgctggat
    31501 gaactcaaca atgtggataa tttccgtcag gtcgtcagca ccaccgcgaa tctgaccacc
    31561 tttaccccta atcctgacag tgaaattgcc cactatgtgg cgcagattca ctctacacga
    31621 cagacaaagg agctttgcgc atgaccactc aactggaaca ggcctgggag ctggcgaaac
    31681 agcgtttcgc ggcggtgggg attgatgtcg aggaggcgct gcgccaactt gatcgtttac
    31741 ccgtttcaat gcactgctgg cagggcgatg atgtttccgg ttttgaaaac ccggaaggtt
    31801 cgctgaccgg tgggattcaa gctactggta attatccggg caaagcgcgt aatgccagtg
    31861 agctacgtac cgatctggaa caggctatgc ggctgattcc ggggccgaaa cggcttaatt
    31921 tacatgccat ctatctggaa tcagacacgc cagtctcgcg cgaccagatc aaacctgagc
    31981 acttcaaaaa ctgggttgaa tgggcgaaag ccaatcagct cggtctggat tttaacccct
    32041 cctgtttttc gcatccgcta agcgccgatg gctttacgct ttcccatgcc gacgacagca
    32101 ttcgccagtt ctggattgat cactgcaaag ccagccgtcg cgtttcggcc tattttggcg
    32161 agcaactcgg cacaccatcg gtgatgaaca tctggatccc ggatggtatg aaagatatca
    32221 ccgttgaccg tctcgccccg cgtcagcgtc tgctggcagc actggatgag gtgatcagcg
    32281 agaagctaaa ccctgcgcac catatcgacg ccgttgagag caaattgttt ggcattggcg
    32341 cggagagcta cacggttggc tccaatgagt tttacctggg gtatgccacc agccgccaga
    32401 cggcgctgtg cctggacgcc gggcacttcc acccgactga agtgatttcc gacaagattt
    32461 ccgccgccat gctgtatgtg ccgcagttgc tgctgcacgt cagtcgtccg gttcgctggg
    32521 acagcgatca cgtagtgctg ctggatgatg aaacccaggc aattgccagt gagattgtgc
    32581 gtcacgatct gtttgaccgg gtgcatatcg gccttgactt cttcgatgcc tctatcaacc
    32641 gcattgccgc gtgggtcatt ggtacacgca atatgaaaaa agccctgctg cgtgcgttgc
    32701 tggaacctac cgccgagctg cgcaagttgg aagcggcggg cgattacact gcgcgtcagg
    32761 cactgctgga agagcagaaa tcgttgccgt ggcaggcggt atgggaaatg tattgtcagc
    32821 gtcacgatac cccagcgggt agcgaatggc tggagagcgt gcgggcttat gagaaagaaa
    32881 ttttgagtca gcgtgggtaa acactgccag atgcggcacg agcgccttat ccggcctaca
    32941 gatcgtgcat ctgacaacac cccatttaca ggaacaccga acatgcaaaa cattactcag
    33001 tcctggtttg tccagggaat gatcaaagcc accaccgacg cctggctgaa aggctgggac
    33061 gagcgcaacg gcggcaacct gacgctacgc ctggatgacg ctgacatcgc accgtatcac
    33121 gacaatttcc accaacaacc gcgttatatc ccgctcagcc aacccatgcc tttactggca
    33181 aatacaccgt ttatcgtcac cggctcgggc aaattcttcc gtaacgtcca gcttgatcct
    33241 gcggctaact taggcgtcgt aaaagtcgac agcgacggcg cgggctacca tattctctgg
    33301 gggttaacca acgaggccgt tccaacttct gaacttccgg cacactttct ctcccattgc
    33361 gaacgcatta aagccaccaa cggtaaagat cgggtgatca tgcactgcca tgccaccaac
    33421 ctgatcgccc tcacctatgt actggaaaac gacacggcgg tcttcactcg ccaactgtgg
    33481 gaaggcagca ccgagtgcct ggtggtattc ccggatggcg ttggcattct gccgtggatg
    33541 gtgcccggca cggacgaaat cggtcaggcg accgcacaag aaatgcaaaa acattcgctg
    33601 gtgttgtggc ccttccacgg tgtgttcggt tccggaccga cactggatga agcctttggt
    33661 ttgatagata ccgcagagaa atcagcggag gtgttagtca aaatctactc gatgggcggt
    33721 atgaagcaga ccattacccg tgaggagctg attgcactcg gtgagcgttt tggcgttacg
    33781 ccactggcca gtgcgctggc actttaatat cccaacagat atccggcggt gtgggaaacg
    33841 tcgccggaca gatggatttg aacttaagga gagtcattat gagttttatg ttggcattac
    33901 cgaaaattag cctgcatggt gctggggcaa ttggcgatat ggtaaatctg gttgcgcata
    33961 aacagtgggg aaaagcactg atcgttaccg atgggcaact ggttaagttg ggattactgg
    34021 acagcctgtt tatcgcactg gatcaatacg aaatgtctta tcacttattt gatgaggtat
    34081 ttcctaaccc cactgaagaa ctggtgcaaa aaggctacgc tgcatttcag gatgccgcat
    34141 gtgactacat tattgctttc ggtggcggta gcccaattga tactgcaaaa gccgtaaaaa
    34201 tccttaccgc taatcctgcg ccttctacgg cgtactctgg tgttggtaaa gtgacaaatc
    34261 ccggcgtacc gctggtggca atcaatacga ctgccggaac agctgctgag atgaccagca
    34321 atgcggtgat catcgacacc gcgcggcagg taaaagaggt cattatcgac ccgaatatta
    34381 ttcccgatat tgcggtggat gatgccagtg taatgctgga gatcccggcc tcggttaccg
    34441 ccgccacggg tatggatgca ctgactcacg cagttgaagc ttatgtttct gttggtgcac
    34501 atccattaac cgacgctaac gcactggaag ctatccgttt aattacgctg tggctgccaa
    34561 aagcggtaga tgatggtcat aatctggaag ctcgtgaaca aatggcattc gcccagtacc
    34621 tggcgggtat ggcatttaac agtgctggtc tgggccttgt tcatgcactt gcgcatcagc
    34681 ccggcgccac ccacaacctg ccgcatggtg tgtgcaatgc tatcttgttg ccagttattg
    34741 aaaactttaa ccgcccgaat gccatcgcac gctttgcccg cattgctcag gcaatgggtg
    34801 tcgatacgcg tgatatgagt gaagaagcgg caagtactga agcaattaac gctatccgcg
    34861 cattaagcca tcgcgtgggt attccggcag gattcagcca gcttggcatc accaaagaag
    34921 atattgaagg ctggttagat aaagccctgg ctgacccgtg tgtgccatgt aacccgcgta
    34981 ccgccagccg cgacgaggtg cgcgagctgt atctggaggc attatgatcc gtaaggcatt
    35041 tgtgatgcag gttaaccccg acgctcacga agagtatcaa cgtcggcata accccatctg
    35101 gccggaactg gaagcagtgt tgaaatctca cggtgcgcat aactacgcca tttacctcga
    35161 caaagcgcgt aatctgctgt ttgccacggt agagattgaa tctgaagagc gctggaatgc
    35221 ggttgccagt actgatgttt gccaacgttg gtggaaatat atgaccgacg tcatgcccgc
    35281 gaacccggat aacagcccgg tgagtagcga actgcaagaa gtgttttacc tgccgtaatt
    35341 gcctaatgtg ccggagacgc ctccggtaca atatttttaa tttcgatttg aaaaataaac
    35401 atattacacc ttcagttaat atatctcggt aacttatatt tccccgatgc attgtgagta
    35461 aatcacaaaa ataatgaata actcattaat gattcatgtg gtttatttaa ataaccatta
    35521 tgtgcattac tccgcaaatc tggcctttca ctctgtctca aattgtcatt gtttaccgac
    35581 atatcacctc tcatcactta atcgctccac cattacaatt gattaaaata tattttatgg
    35641 agtccgatta atggcagctc ttactgcaag ctgtattgac ctgaatattc agggcaatgg
    35701 cgcttattcc attctgaagc agttggcgac aatggcgtta caaaacggtt ttatcaccga
    35761 ctcacaccag ttcctgcaaa ccctgctgct gcgcgaaaaa atgcactcta ctggatttgg
    35821 ttccggcatc gccgtgccgc acggtaaaag cgcctgcgtt aaacaaccgt tcgtgttatt
    35881 cgcccgcaaa gcgcaggcca ttgactggaa agccagcgac ggcgaagacg tcaattgctg
    35941 gatctgcctc ggcgtgccgc aaagtggcga agaggatcag gtcaaaatca tcggcacact
    36001 gtgtcgcaaa attatccatc aggattttat caaccaactg cgacagggcg acgccgacca
    36061 ggtacttgct ttgttaaatc aaaccctcag ctcataagga agtggcgatg gagtcatcct
    36121 tacgtattgt cgcgatcacc aactgccccg ccgggatcgc tcacacctac atggtggcgg
    36181 aagccctgga acagaaagcc cgttctctcg gccataccat aaaagtggaa actcaagggt
    36241 ccagcggcgt tgaaaaccgc ttatccagcg aagagattgc cgctgccgat tacgtcattc
    36301 tagctaccgg gcgtggcctg agcggtgatg accgggcgcg gtttgccggg aagaaagttt
    36361 atgagattgc catctcccag gcgctgaaaa atatcgacca gattttcagc gaattaccaa
    36421 caaactcgca gctttttgcc gcagatagcg gcgtgaagct cggcaaacag gaagtgcaaa
    36481 gcggcagcgt gatgagccac ctgatggcgg gcgtctctgc ggcgctgcca tttgtcatcg
    36541 gcggcggtat tctggtggcg cttgccaaca tgctggtgca gttcggtttg ccatataccg
    36601 atatgtcgaa aggtgcgccg tcgtttacct gggtagttga atccatcggt tacctcggct
    36661 tcacctttat gatccccatc atgggcgctt atattgcctc gtcgattgcc gataaacccg
    36721 ctttcgctcc ggcatttctg gtttgctatc tggcgaacga taaagccctg ctcggcaccc
    36781 agtcaggcgc gggcttcctc ggcgcggtgg tgctggggct ggcgattggc tatttcgtct
    36841 tctggttccg taaagtccgt cttggcaaag cattacaacc gctgctcggc tccatgctta
    36901 tcccattcgt tactctgctg gtttttggcg tgctcactta ctacgttatc gggccggtga
    36961 tgtccgacct catgggcggg ctgctccact tcctgaatac cattcctcca tcaatgaagt
    37021 ttgcggcagc gtttctggtc ggtgcgatgc tggcgttcga tatgggcggc cccatcaata
    37081 aaaccgcctg gttcttctgc ttctcactgc tggaaaaaca catttatgac tggtacgcca
    37141 tcgtcggcgt tgtcgcgctg atgccgcccg tcgcggcggg tcttgcgacg ttcattgcgc
    37201 ccaaactgtt tacccgacag gaaaaagagg ctgccagtag tgccattgtg gtcggtgcca
    37261 cagtcgcaac cgaaccggct attccttacg ctctggcagc tccgttgccg atgattaccg
    37321 ccaatacgct ggcgggcggt attaccggcg tgctggtgat cgctttcgga atcaaacgtc
    37381 tggcacctgg tttgggtatc tttgacccgc tgatcggcct gatgtcaccg gtaggttcgt
    37441 tttatctggt gctggcgatt ggcctcgcgc tgaacatttc attcattatc gtcctgaaag
    37501 gattgtggtt acgccgcaaa gcgaaagccg cgcaacagga gcttgtccat gaacattgag
    37561 ttactgcaac agttgtgcga agccagcgcc gttagcggcg atgaacagga agttcgcgac
    37621 attctgataa acactctgga accttgcgtg aatgaaatca cctttgatgg gctgggcagc
    37681 tttgtcgccc gtaagggaaa taaagggccg aaagttgccg ttgtcgggca tatggatgaa
    37741 gtcggcttta tggtcaccca catcgacgag agcggttttc tgcgtttcac caccattggt
    37801 ggctggtgga atcagtcgat gctcaaccac cgggtaacca tacgcacaca caagggagtg
    37861 aaaatccctg gtgtgattgg ttccgtcgcg cctcatgcgt taacggaaaa gcaaaagcaa
    37921 cagccgcagt catttgatga gatgttcatt gatattggcg caaacagtcg cgatgaggtg
    37981 gaaaaacgcg gtgtggcgat gggcgatttt attagcccgg aagccaattt tgcctgctgg
    38041 ggcgaagata aagtggtcgg caaggcgttg gataaccgca tcggctgcgc aatgatggct
    38101 gagctactac agacggtgaa taatcccgaa attacgctgt atggcgttgg cagtgtggaa
    38161 gaagaagttg ggctacgcgg ggcgcaaacc tcggctaaac acattaaacc ggatgtggtg
    38221 atcgtgctgg acaccgctgt cgcgggcgat gttccaggca ttgataacat taaatatccg
    38281 ctgaaactgg gcaacgggcc ggggttgatg ctgtttgaca agcgctactt ccccaaccag
    38341 aaactggtag cagcgttaaa aagctgtgcc acgcagaatg atttaccgct gcaattttcc
    38401 accatgaaaa ccggtgcgac ggatggcggg cgctacaacg tgatgggcgg tgggcgtccg
    38461 gttgtcgcgc tgtgtttgcc aacgcgttat ctgcacgcca acagcggcat gatttcaaaa
    38521 gccgattacg atgctctgct cacgctgata cggggttttc tgaccacctt aaccgcggaa
    38581 aaagtcagcg cgtttagcca gttccgtcag gtggattaat gctaaacgaa cgccagttaa
    38641 agattgtcga tctgctggag caacagccgc gcacgcctgg cgagctggcg caacagactg
    38701 gcgtttctgg caggaccata ctgcgtgata ttgactatct caactttacc cttaacggca
    38761 aagcccgcat ttctgccagt ggtagtgcgg gctatcagct ggaaatcttc gagcgccgca
    38821 gcttttttca gttgctgcaa aagcacgata acgacgatcg gctgctggca ctgttattac
    38881 tgaatacctt tactccccgt gcgcaactcg cctcggcgct taatttgccg gaaacgtggg
    38941 tcgcagagcg tctgccccgg ttaaaacagc gttatgaacg cacttgctgc ctggccagtc
    39001 gccccggttt gggccatttc attgatgaga cagaagaaaa acgcgttata ttgctggcga
    39061 acttgctgcg caaagatccg tttttaattc cgctggcggg cgtaacacga gacaaccttc
    39121 agcatttatc cacggcctgc gacaaccaac accgctggcc gctcatgcag ggtgattatc
    39181 tctccagcct gattctggcg atttacgccc tgcgtaatca actgaccgat gagtggccgc
    39241 aatatcccgg tgacgagata aaacaaatcg ttgaacatag cggtctgttt cttggtgata
    39301 acgctgtaag aaccctgaca ggtttgatag agaaacagca tcagcaagcg cagataattt
    39361 ctgccgataa tgtgcagcgg ttgctgcaaa gggtgccggg catcgcgtca ttgaatatta
    39421 ttgatacgca gctggttgag aacattaccg ggcatttatt acgttgcctt gccgcaccgg
    39481 tgtggattgc tgagcaccgc caaagcagca tgaataacct gaaagccgcc tggcctgcgg
    39541 cctttgatat gagtctgcac tttattacgc tactgcgtga acagctcgat attccccttt
    39601 tcgacagcga tctgatcggt ttgtattttg cctgtgcgct ggaacggcat caaaacgaac
    39661 gccagccgat cattttgctc tcggaccaga acgcgattgc cactattaat cagctcgcca
    39721 ttgagcgcga tgttttacat tgtcgggtga ttattgcccg tagcttaagc gaacttgttg
    39781 ccattcgcga agagattgag ccgttattga tcattaacaa cagccattat ttactggatg
    39841 acgcggtaaa taattacatc accgtaaaaa atatcattac ggctgccggt atcgaacaaa
    39901 taaaacattt tctggcgacg gcatttattc gccaacagcc ggagcgtttt ttctctgctc
    39961 ccggaagttt tcattattcg aatgtacgcg gtgaaagctg gcaacatatt acccggcaaa
    40021 tttgtgcgca attagtcgca caacaccata ttaccgccga tgaagcacaa cgcatcatcg
    40081 cccgcgaagg cgaaggtgaa aacctgattg ttaatcgcct cgccatccca cattgctgga
    40141 gcgaacagga gcgacgtttt cgtggatttt ttattaccct cgcccaacca gttgaggtga
    40201 ataacgaagt cattaaccat gtcttgatcg cctgcgccgc cgccgatgcg cgtcatgagc
    40261 taaaaatatt tagctatctg gcaagcatat tgtgtcagca tccggcggag attattgccg
    40321 ggttaacagg atatgaggca tttatggagt tacttcacaa ggggtgaact gtgtcggatg
    40381 cgccgcatcc gacactgcat ttatcatcaa aaacgtttaa cgcaggctat tatagtcatc
    40441 aaccatttcg ttgtactcgc gtaatgctct cgggaaagag tcttcgacca tccagcttga
    40501 gtttgcgtcc tgaagttgct ctttatccca gtcagagtac gggactttat ctctgatgcg
    40561 gcgaacgtat tttttagcct cgagttgata ctggcctgcc gaattaataa acgagaaatt
    40621 catgccgcct ttatccgctt ctttggcctg cgccactagc gtttccagtt cagacacttt
    40681 cttcattgcc gcatcaacat caaacttctc ttgcgatatc aggttattaa tttgtttcgc
    40741 tgaaatcatg actgccagag agtagtagtg gaatgttttc ccttctcttt cttcaatatt
    40801 tttcagttcc gcaagctggc gcttatcgtt aatttcctga atcgccgcat gataagattc
    40861 tgctacaggt tcaaaggctt ccagattttt taagaatgtc tggtgcagcg ttttcccttt
    40921 cgcaaacgca tcgtctttat aattttcctg ggtgtaatat ttatccattt cgttaatggt
    40981 attacccaac gccacggcag catcgatata actcaccgcc acgccatcaa ttggttgcag
    41041 cgcaggcgtt aacgccacag cactttttac gcctttttca cactcagcga ggttggattc
    41101 actaatgccg tagatgccat aaacagtacg ctcttcaccg gtaggcccct gtttaaaatc
    41161 tttcagccag tcagcataac gcgccagact gctctgtaca gggatctgta acttgttata
    41221 gcacttgata tagacatcca ttttttcgtc gaaaaccgct tgttcatcga ccaccggttg
    41281 cgcaggtgtt tcaggtttag cttctgcttt tgcaacggga gcttcaggag ctggtgcggc
    41341 aggttgacta ttggcaggcg ggggcgtttc tgtttcggct tttttatcat cacaacccgt
    41401 cagaccgagg gccatgatgg cgactataat agcggaagat aataaattcc ttttca
//
LOCUS       DAZFWL020000025        40425 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFWL020000025 DAZFWL020000000
VERSION     DAZFWL020000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.025"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..584)
                     /locus_tag="ACHKNP_RS07420"
                     /old_locus_tag="pgaptmp_001484"
     CDS             complement(<1..584)
                     /locus_tag="ACHKNP_RS07420"
                     /old_locus_tag="pgaptmp_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGU4703679.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQKITELIEVVYNRRVDR"
     gene            complement(844..2097)
                     /gene="amiC"
                     /locus_tag="ACHKNP_RS07425"
                     /old_locus_tag="pgaptmp_001485"
     CDS             complement(844..2097)
                     /gene="amiC"
                     /locus_tag="ACHKNP_RS07425"
                     /old_locus_tag="pgaptmp_001485"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HGU4703680.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2329..3660
                     /gene="argA"
                     /locus_tag="ACHKNP_RS07430"
                     /old_locus_tag="pgaptmp_001486"
     CDS             2329..3660
                     /gene="argA"
                     /locus_tag="ACHKNP_RS07430"
                     /old_locus_tag="pgaptmp_001486"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HGU4703681.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3722..5548)
                     /gene="recD"
                     /locus_tag="ACHKNP_RS07435"
                     /old_locus_tag="pgaptmp_001487"
     CDS             complement(3722..5548)
                     /gene="recD"
                     /locus_tag="ACHKNP_RS07435"
                     /old_locus_tag="pgaptmp_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HGU4703682.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMVL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVVSGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5548..9090)
                     /gene="recB"
                     /locus_tag="ACHKNP_RS07440"
                     /old_locus_tag="pgaptmp_001488"
     CDS             complement(5548..9090)
                     /gene="recB"
                     /locus_tag="ACHKNP_RS07440"
                     /old_locus_tag="pgaptmp_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HGU4703683.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEELELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(9083..11971)
                     /gene="ptrA"
                     /locus_tag="ACHKNP_RS07445"
                     /old_locus_tag="pgaptmp_001489"
     CDS             complement(9083..11971)
                     /gene="ptrA"
                     /locus_tag="ACHKNP_RS07445"
                     /old_locus_tag="pgaptmp_001489"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HGU4703684.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQHLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEDQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12147..15515)
                     /gene="recC"
                     /locus_tag="ACHKNP_RS07450"
                     /old_locus_tag="pgaptmp_001490"
     CDS             complement(12147..15515)
                     /gene="recC"
                     /locus_tag="ACHKNP_RS07450"
                     /old_locus_tag="pgaptmp_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HGU4703685.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLADDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSVEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPDDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15528..15851)
                     /gene="ppdC"
                     /locus_tag="ACHKNP_RS07455"
                     /old_locus_tag="pgaptmp_001491"
     CDS             complement(15528..15851)
                     /gene="ppdC"
                     /locus_tag="ACHKNP_RS07455"
                     /old_locus_tag="pgaptmp_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGU4703686.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15836..16243)
                     /gene="ygdB"
                     /locus_tag="ACHKNP_RS07460"
                     /old_locus_tag="pgaptmp_001492"
     CDS             complement(15836..16243)
                     /gene="ygdB"
                     /locus_tag="ACHKNP_RS07460"
                     /old_locus_tag="pgaptmp_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HGU4703687.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16240..16803)
                     /gene="ppdB"
                     /locus_tag="ACHKNP_RS07465"
                     /old_locus_tag="pgaptmp_001493"
     CDS             complement(16240..16803)
                     /gene="ppdB"
                     /locus_tag="ACHKNP_RS07465"
                     /old_locus_tag="pgaptmp_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU4703688.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16794..17264)
                     /gene="ppdA"
                     /locus_tag="ACHKNP_RS07470"
                     /old_locus_tag="pgaptmp_001494"
     CDS             complement(16794..17264)
                     /gene="ppdA"
                     /locus_tag="ACHKNP_RS07470"
                     /old_locus_tag="pgaptmp_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU4703689.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17448..18242)
                     /gene="thyA"
                     /locus_tag="ACHKNP_RS07475"
                     /old_locus_tag="pgaptmp_001495"
     CDS             complement(17448..18242)
                     /gene="thyA"
                     /locus_tag="ACHKNP_RS07475"
                     /old_locus_tag="pgaptmp_001495"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGU4703690.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18249..19124)
                     /gene="lgt"
                     /locus_tag="ACHKNP_RS07480"
                     /old_locus_tag="pgaptmp_001496"
     CDS             complement(18249..19124)
                     /gene="lgt"
                     /locus_tag="ACHKNP_RS07480"
                     /old_locus_tag="pgaptmp_001496"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGU4703691.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19275..21521)
                     /gene="ptsP"
                     /locus_tag="ACHKNP_RS07485"
                     /old_locus_tag="pgaptmp_001497"
     CDS             complement(19275..21521)
                     /gene="ptsP"
                     /locus_tag="ACHKNP_RS07485"
                     /old_locus_tag="pgaptmp_001497"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4703692.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGCEVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21534..22064)
                     /gene="rppH"
                     /locus_tag="ACHKNP_RS07490"
                     /old_locus_tag="pgaptmp_001498"
     CDS             complement(21534..22064)
                     /gene="rppH"
                     /locus_tag="ACHKNP_RS07490"
                     /old_locus_tag="pgaptmp_001498"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HGU4703693.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22417..22563)
                     /gene="ygdT"
                     /locus_tag="ACHKNP_RS07495"
                     /old_locus_tag="pgaptmp_001499"
     CDS             complement(22417..22563)
                     /gene="ygdT"
                     /locus_tag="ACHKNP_RS07495"
                     /old_locus_tag="pgaptmp_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HGU4703694.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22749..23438
                     /gene="mutH"
                     /locus_tag="ACHKNP_RS07500"
                     /old_locus_tag="pgaptmp_001500"
     CDS             22749..23438
                     /gene="mutH"
                     /locus_tag="ACHKNP_RS07500"
                     /old_locus_tag="pgaptmp_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HGU4703695.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23507..24220
                     /gene="ygdQ"
                     /locus_tag="ACHKNP_RS07505"
                     /old_locus_tag="pgaptmp_001501"
     CDS             23507..24220
                     /gene="ygdQ"
                     /locus_tag="ACHKNP_RS07505"
                     /old_locus_tag="pgaptmp_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU4703696.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24358..24576
                     /gene="ygdR"
                     /locus_tag="ACHKNP_RS07510"
                     /old_locus_tag="pgaptmp_001502"
     CDS             24358..24576
                     /gene="ygdR"
                     /locus_tag="ACHKNP_RS07510"
                     /old_locus_tag="pgaptmp_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HGU4703697.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24684..25724
                     /gene="tas"
                     /locus_tag="ACHKNP_RS07515"
                     /old_locus_tag="pgaptmp_001503"
     CDS             24684..25724
                     /gene="tas"
                     /locus_tag="ACHKNP_RS07515"
                     /old_locus_tag="pgaptmp_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HGU4703698.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25756..26949)
                     /gene="lplT"
                     /locus_tag="ACHKNP_RS07520"
                     /old_locus_tag="pgaptmp_001504"
     CDS             complement(25756..26949)
                     /gene="lplT"
                     /locus_tag="ACHKNP_RS07520"
                     /old_locus_tag="pgaptmp_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HGU4703699.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26942..29101)
                     /gene="aas"
                     /locus_tag="ACHKNP_RS07525"
                     /old_locus_tag="pgaptmp_001505"
     CDS             complement(26942..29101)
                     /gene="aas"
                     /locus_tag="ACHKNP_RS07525"
                     /old_locus_tag="pgaptmp_001505"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HGU4703700.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29687..30718
                     /gene="galR"
                     /locus_tag="ACHKNP_RS07530"
                     /old_locus_tag="pgaptmp_001506"
     CDS             29687..30718
                     /gene="galR"
                     /locus_tag="ACHKNP_RS07530"
                     /old_locus_tag="pgaptmp_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HGU4703701.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLTSLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30725..31987)
                     /gene="lysA"
                     /locus_tag="ACHKNP_RS07535"
                     /old_locus_tag="pgaptmp_001507"
     CDS             complement(30725..31987)
                     /gene="lysA"
                     /locus_tag="ACHKNP_RS07535"
                     /old_locus_tag="pgaptmp_001507"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGU4703702.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            32109..33044
                     /gene="lysR"
                     /locus_tag="ACHKNP_RS07540"
                     /old_locus_tag="pgaptmp_001508"
     CDS             32109..33044
                     /gene="lysR"
                     /locus_tag="ACHKNP_RS07540"
                     /old_locus_tag="pgaptmp_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HGU4703703.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(33031..33723)
                     /gene="ygeA"
                     /locus_tag="ACHKNP_RS07545"
                     /old_locus_tag="pgaptmp_001509"
     CDS             complement(33031..33723)
                     /gene="ygeA"
                     /locus_tag="ACHKNP_RS07545"
                     /old_locus_tag="pgaptmp_001509"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HGU4703704.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33852..35270)
                     /gene="araE"
                     /locus_tag="ACHKNP_RS07550"
                     /old_locus_tag="pgaptmp_001510"
     CDS             complement(33852..35270)
                     /gene="araE"
                     /locus_tag="ACHKNP_RS07550"
                     /old_locus_tag="pgaptmp_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HGU4703705.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(35585..36346)
                     /gene="kduD"
                     /locus_tag="ACHKNP_RS07555"
                     /old_locus_tag="pgaptmp_001511"
     CDS             complement(35585..36346)
                     /gene="kduD"
                     /locus_tag="ACHKNP_RS07555"
                     /old_locus_tag="pgaptmp_001511"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HGU4703706.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(36376..37212)
                     /gene="kduI"
                     /locus_tag="ACHKNP_RS07560"
                     /old_locus_tag="pgaptmp_001512"
     CDS             complement(36376..37212)
                     /gene="kduI"
                     /locus_tag="ACHKNP_RS07560"
                     /old_locus_tag="pgaptmp_001512"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HGU4703707.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(37499..38680)
                     /gene="yqeF"
                     /locus_tag="ACHKNP_RS07565"
                     /old_locus_tag="pgaptmp_001513"
     CDS             complement(37499..38680)
                     /gene="yqeF"
                     /locus_tag="ACHKNP_RS07565"
                     /old_locus_tag="pgaptmp_001513"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGU4703708.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38935..40164
                     /gene="yqeG"
                     /locus_tag="ACHKNP_RS07570"
                     /old_locus_tag="pgaptmp_001514"
     CDS             38935..40164
                     /gene="yqeG"
                     /locus_tag="ACHKNP_RS07570"
                     /old_locus_tag="pgaptmp_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4703709.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT        10056 a        10944 c         9762 g         9663 t
ORIGIN      
        1 cttctgtcca cccttctgtt ataaacaact tcaataagct ccgtgatttt ttgagacagc
       61 tcaataatcc ttactgattc ataatcaact gaattttcat aattatttaa aatatgcaat
      121 gctataaaac caatctcatc atccggtagt tgaacgctaa attgctcatt aatgattgtt
      181 aatgcatcac gacttattgc atactctgct ttgtacaacg ctttgatttc ctcaaggaaa
      241 acattattaa tttgtatgcc tgagacgact cgggatattg cattcgaaat gtgatcagca
      301 agaatcagta gtagcgatgg attgagtttt ttttcgagat tactctccgc ataatgcact
      361 atcttttcgg caacaaaaat atactcaata cccacatgct caatcaattt gtagagttta
      421 tttttttgct catttctgac ataaaaaatc cggtttgcag gatgctcagg gaccgacata
      481 ccatattttt tattgtaacc aacgccaggc ccagaaatga taacctcctg tccgttcacc
      541 gatgcctgta cacaattatt gttcatgact ttttcgatga tcattccatg gctcccaaaa
      601 aaaaaggcaa ggcatgtcag aagtaataat tacttcaaac attgccttgc ctgatttaac
      661 agttacaaga taattaacta atgcgtatac cttacacgga attgtgctta ataaaagaga
      721 tgacaggctt attttttaag ccgatattca aatattcgac agcagtcaca aaaccatcgt
      781 tcaacattta tgtaaataat atttattcaa tgaaacataa attaattgaa taaattaaaa
      841 acatcatccc cttctcgcca gcgtcgcccc atcggcaaaa tacgctttaa tccccgcaag
      901 aatagactcc gcaacttcct gctgaaaggt cgccgttttc agtttacgct cttcctcaac
      961 gttactgata aacgccgttt cgaccagaat ggagggaata tctggtgcct ttagtacggc
     1021 aaacccggcc tgttcaacct gatttttatg cagcttgttg attttaccga gcttattcag
     1081 taccgcttta ccaaacttaa ggctgtcagc aatggtcagc gactgcacca tatcgaacat
     1141 ggtgtggtcg acatagcggt caccgctttt gctcacgcca ccaatcaagt ccgaggcgtt
     1201 ctgggtttgt gccagatatt ttgccgcagt actggttgca cctttggttg agagcgcaaa
     1261 cacagaggaa ccgctcggct gacgactggt aaaggcgtcg gcatggatag agacaaacag
     1321 gtcagcacgc tgtttctggg cttttgctac gcgcacttgc aacggaatga agatgtcttc
     1381 attgcgcgtc atgtacacct tcatattgcc ctctttctcg atcagagagc gcaaacggcg
     1441 agctatttgc aataccacat ctttttctcg cgttttgtat ttccccaccg caccggagtc
     1501 ttcgccaccg tggccagggt caagcataat gacaatcgga cgatcgcgcc ctgctttacc
     1561 cggttgtgga ccactttgtg ctggcggcac ctgcttttcg aggtcgcctt tgttgtaatc
     1621 ctccagcagc gccagcagcg ggtcctgcat atcctgtgca ttggccggat agaggtccat
     1681 caccagacgc tctttaaacc cggcgaccgg cgcaagggca aacagctgcg gttttacgtt
     1741 ttgctttaat tcaaaaacca tccgtacggt ctgcgggtca aattgcccga cgcgcgccga
     1801 cttgatgaac gggtcgtctg cacgaatttg tgccgccatc cccttgagta ccgagttcag
     1861 gtttacatct tcgatatcca ccaccacgcg ttccgggtta ctcaacgcga actgcttata
     1921 tttcagctga cgatttgatt ctaccgtcac acgggtgtag ctggacgcag gccagacgcg
     1981 caccgccacg acctggctga ccgcagccag actgacctga cttacgctca atagccacat
     2041 ggcacccgcg ccttgcagta aacgacgacg gctgattgca gtgttggatc ctgacatgcc
     2101 tctcccgagc aaaagaaatc taagctgtgt aacaagtaaa cgactaattt gaccggaaac
     2161 tttagcgaaa gacgcataat ctgtcatcta ataaacggta aacattcttt ttatattcac
     2221 ggcattactg ataaaaaagt cgctctcgca taaaatttac acttgcaccc tgcgaaaaaa
     2281 cagaataaaa atacactaat ttcgaataat catgcaaagg ggtgtgccgt ggtaaaggaa
     2341 cgtaaaaccg agctggtcga gggattccgc cattcggttc cctatatcaa tacccaccgg
     2401 ggaaaaacgt ttgtcatcat gctcggcggt gaagccattg agcatgagaa tttctccagt
     2461 atcgttaatg atatcgggtt gttgcacagc ctcggcatcc gtctggtggt ggtctatggc
     2521 gcacgtccgc agatcgacgc aaatctggcc gcgcatcacc acgaaccgct gtatcacaag
     2581 aatatacgtg tgaccgacgc caaaacactg gaactggtga agcaggctgc gggaacattg
     2641 caactggaca tcactgctcg cctgtcgatg agtctcaata acacgccgct acagggcgcg
     2701 catatcaacg tcgtcagtgg caattttatt attgcccagc cgctgggcgt cgatgacggc
     2761 gtggattact gccatagcgg gcgtatccgg cggattgatg aagacgcgat ccatcgtcaa
     2821 ctggacagcg gtgcaatagt gctaatgggg ccggtcgctg tttcagtcac tggcgagagc
     2881 tttaatctga cctcggaaga gattgccact caactggcca tcaaactgaa agctgaaaag
     2941 atgattggtt tttgctcttc ccaaggcgtc actaatgacg acggtgatat tgtctccgaa
     3001 cttttcccta acgaagcgca agcacgggta gaagcccagg aagagaaagg cgattacaac
     3061 tccggtacgg tgcgcttttt gcgtggcgca gtgaaagcct gccgcagcgg cgtgcgtcgc
     3121 tgtcatttaa tcagttatca ggaagatggc gcgctgttgc aagagttgtt ctcacgcgac
     3181 ggtatcggta cgcagattgt gatggaaagc gccgagcaga ttcgtcgcgc aacaatcaac
     3241 gatattggcg gtattctgga gttgattcgc ccgctggagc agcaaggtat tctggtgcgc
     3301 cgttctcgcg agcagctgga gatggaaatc gacaaattca ccattattca gcgcgataac
     3361 acgactattg cctgcgccgc gctctatccg ttcccggaag agaagattgg ggaaatggcc
     3421 tgtgtggcag ttcacccgga ttaccgcagt tcatcacggg gcgaggttct gctggaacgc
     3481 attgccgctc aggcgaagca gagcggctta agcaaattat ttgtgctgac cacgcgcagt
     3541 attcactggt tccaggaacg tggatttacc ccagtggata ttgatttact gcccgagagc
     3601 aaaaagcagt tgtacaacta ccagcgtaaa tccaaagtgt tgatggcgga tttggggtaa
     3661 gcgatgaaaa tcgccggatg cgacatgcgt aacactcgtg cgtcgcatca ggcaattacg
     3721 tttattcccg cgaactaaac aatgccgcca gaccactgcg ccgctcagtg cgcgtggcga
     3781 ttgccgcact taatatgcgc tcatcggcat acagcgacag acggcgacgc gcgcgggtca
     3841 ctgcggtgta aaccagctct cgcgttacta ccggcgtgcg ttggctcggt aaaatcaacg
     3901 ccgcatggtc gaactccgat ccctgcgatt tatgtaccgt catcgcccac gtcgtttcgt
     3961 gctctggcag gcggctcggt tgcacagact taatattgcc gtccggcatc gcaaaccaaa
     4021 cgcgcgtccc ctgcccgcga tccagcgcaa taccgatatc gccattaaac aacccaagcg
     4081 cgctgtcatt acgggcaatc atcaccggtc gaccttcgta ccaacgagag tgcggatgac
     4141 gatgaatttt gcgcttctgt tgcataaact gctcaattcg ctcattcagt ccagccacgc
     4201 caaacggccc ttcccgcagg gcgcacaaaa gttggtactc attgaacgcc tgaatgatta
     4261 aatccggctc ggcacgcgct tgcagcagat ccagataacg tccataaccc gcaagagctt
     4321 cctcaagcat cgcaatataa tcttctccgc tctgtaaaag tcgtttttcg atatcagtaa
     4381 aatcctgctg aaaaacggtt ttcactgccg ttttatcacc acggttgatc gccgcagcta
     4441 actgaccaat gccagaatcg ctgccgaaac gatagctttt ttgcagcagg cagagactgt
     4501 cgcgcaaaga tgccgcttct gtgccggttc ctgccggaac gtgagtcccc gtcaggcggc
     4561 ttagctgcct ggcacgctcg gcggtaaagc ccgcgttggc ataagcgcag atatcgccca
     4621 gcacagcccc agcctcaacc gaggccagtt gatcacgatc gccgagaaag atcactcgcg
     4681 catgatctgg caaggcgtcg atcagtctcg acatcatagg cagatcgatc attgacgctt
     4741 catctaccac cagcacatca agatgcagcg ggttaccggc atgatgacgt aaacgctggc
     4801 tacccggctg cgcgcccagc aatcggtgca aagtgctggc atcttccgga atgcgtttct
     4861 tttgttcatc ggtcagcggt aactgtcgca aagccttgcc gagagattcg gttaagcgcg
     4921 cggcagcttt acccgttggt gcagccagac ggatacggca gcgttcgccg tcggccattt
     4981 gaattaacgc tgccagcaac ttcgccacgg tggtcgtttt accggtgcct gggccgccgg
     5041 aaaccaccga gatccgccgc gtcagcgcca ctgccgctgc aactttttgc cagttaattt
     5101 catcgcttac tggaaaaagt ttgtccaggg tttgcgccag tagagcttca tcaacctcaa
     5161 tggcatgatt cacctcattg aagaagcgcg ctaccgtccg ctcgttacac cacatgcgat
     5221 tcaaataaag acgatcgcca cagaggacca tcggcgtggg ttcatctccc ctgctgaccg
     5281 cttgagaagc cagcaagcat tcttcccaat tttgtagctc accgatttca ctgacacagg
     5341 tcgccaacag cggatgcgac gcctcgttat tttccagtcg tgaaagcggc aaacagacgt
     5401 gtccctctcc ggcatcatga cttaacagtg ccgccgcgag ggtgacggca ggatgttcat
     5461 ctcccgccac ggtcagggca aattgcacat ccagcgggcg tagctgtttg tgctccacag
     5521 cttccagtaa ttgcttttgc aatttcatta cgcctcctcc agggtcatac cggcaaacat
     5581 ctcatccatc agggcaatca acccggcgtt gggtcgggtt gtgtaaatcc cttgttgcgg
     5641 atgttcttta tcaacgccac gcaggaacag atagataacg ccgccaaagt gacgatcata
     5701 gtcgtaatca gcaatgcgat ggcgcagata acgatgcagc gccagggtat aaagctgata
     5761 ttgcagatca tagcggtgtg cctgcatcgc tgctgccata gcctgttggg tgtaagccga
     5821 actgtcttca cccagccagt tggatttata gtcgagcagg taataacgcc cttcgtggcg
     5881 gaataccagg tcgataaagc cttttaacat gccgcgtacc tgcatgaact ccagcggcgg
     5941 gcagccagcg gatagcgggt caaactggcg aattagtgca tcaagctggc tggcgatgag
     6001 cggttcgcta atcggcaggt aaaactccat ctccacctgt ttatcgcggt cggaaagctg
     6061 actcaggcta acacccgttt cattgagagg tgcctggagg acagccgtga tccactcggt
     6121 caacaccggt tcccactgcg attcaaagcc gccgagttcc agttcttcct gtacccagtt
     6181 cgggtcaacc ggctgggtaa aatcgaggtc ttcaaacaaa ctgtgcaaga atgtccccgg
     6241 tgacgcaccg cgcgggaact gatgcggtgt taacgtcggt tcttcaacga cgctgaccac
     6301 gcctgcggca tcaacatcca gccgtggcat cagatcctga gcgataccgt gaccacgctg
     6361 ctgcaagccg gagtagctgg tgacacgcca gttatcgccg ggcaatcgtt gtagcgtcct
     6421 cgcattcagt tctgcagtta aagcatcatt aacctgccag ggctggttat caccagtttg
     6481 tgccgtttgc caggcaatat catcatcgca taacgcttca atacaggtgc gaagccctgc
     6541 cgcatcttgc ggttcccctt tttgcagcaa acgcccgagc gcactttggt ggacgtcggt
     6601 gtcacctttt ttatcgccac gacggcgcac cagcggtgca acgccgagac tgcaatgcca
     6661 aaccgaacgc gtcagcgcca cgtaaagcaa acgcagatct tccgccagac gttcgacctc
     6721 cgcaagctcg acgctttctg gcgcagcgtt aagatccaga acagcctcaa acgagtggcg
     6781 atcgtgataa aacgcctgat cctggacgcg gaaattggta ataaatggca accagaccag
     6841 cggatattcc agccctttcg atttatggat cgtgacaatc tgcaccagat gtttatcact
     6901 ttcgaggcgc atttgttggc tggaggcatt actgtctggc tcgaggatat gttgcgataa
     6961 ccagcgtacc agcgcatgtt cactttccag ctgcgttccg gcttcttgta gcagttcgct
     7021 gatatgcaag atatcggtaa gacgccgctc accgcctgcc gttgccagca agttttctgc
     7081 aatgttacgc gccgacatca gcgcccgcag catcggcata acgccacgtt tgcgccagat
     7141 ttgccgataa ccatcgaact cttcaactac cgcatcccac gcatgttcgt cattattcag
     7201 cgtttcgata tccagcgcgt tcagccccat cattgacgtt gccagcgcac tgcgcagggt
     7261 gttctcacgt tcgggcgtca tcaccgcctg caacaaccaa agcatttcct gcgcttccag
     7321 agtttcaaaa acactgtcgc ggttcgaaag gtaaacggaa gggatttcca gcaacgttaa
     7381 ggcatcgcgc acctgggcgg cctcctggcg gctgcgcacc agcacactga tgtccgaagc
     7441 acgcaccgga cgcgcgtcgt cgccgttcat cagcaacgct tcgccccgct gtccggcttg
     7501 tagccagtcg cggatttgcg cagcacatac ctgcgccatg gtactttgat aatcgccaac
     7561 gccgcagctt tcgccttcca tcagccacat tttcatcgca ggctgcgttt cacctttaaa
     7621 cacaaaacgt aacgcctgat ttttcccggc tgatttcact ggaataaacg gtatttcgcg
     7681 aaacatgaac gcgtcatcag tctggctgaa aagcttgttc acactgttca ccattccagg
     7741 tgcggagcgc cagttggtgt ctaaagtgta gtgggcgtga acttcactac gcgccttcat
     7801 ataagtgaag atatccgcac cccggaatgc atatatggcc tgcttcgggt cgccaatcag
     7861 caacaatgcg gtttccggct gatggtgcca gatacggcga aaaattcggt actgctgggg
     7921 gtcggtatcc tgaaattcat cgatcattgc caccgggaat cgcgtacgga tcgccgctgc
     7981 caacacctca ccgctttcgc tacgcagcgc ggaatcgagc cgacttaaca tgtcatcaaa
     8041 acccaattcg ccacggcggc gtttttcacg cgctactgtt tcgcggatct cagccaatgc
     8101 gcgggtgatc accagatcgc ggatcgacaa tggttctgca agcagttgat cgatcgcctc
     8161 aaacagtgga tgtcgcgggg tttccccccc ggccttcgtg cgatcttcta agaaacgctg
     8221 ggagaatttt tccagcgact ccggcaactg ataactgttt gtctcttctt ctgcccaggc
     8281 gctgatcttt tcgatccatt tagcctgatt gctacggtta aacttgcgtc gatcaatacc
     8341 agaagattcg atcagcgcat caagttcacc cactgcgtcg cgccactgct gttttaccgt
     8401 atcaatacgc gccacaattt gcgcgtggcg ggaagccagc gtttcatcat cgggcggcgg
     8461 tgctttgata accggtgctt cgccttgcag ataacgatta atatcgcgca gcaacgcctg
     8521 cggccctttc caggtttcaa agaccaccag ggcaatttca cgcggcagcg ggtagcagtg
     8581 gcgacgccag aaatcggcgc aggcctggta gcgtagcaga gactcatctt caatcagctg
     8641 ctgctcaaac agcataccgg attcaaaggc attcaggttg agcatgcgct ggcaaaaacc
     8701 gtgaatagta aagactgccg cttcatccat ctgccgttcg gctaacaaca accactgcgc
     8761 ggcttgcgct ttatcgtcga tctcttccag caggcgttcg tacagtggat tgtcggtggt
     8821 ttcacgcaga caggcgatgc gcaactcgtg gatattgcta cggatacgac cacgcaattc
     8881 tgccgtggca gcctcggtaa aggtgaccac cagcagttct tcaacggtca gcgggcgggg
     8941 aaaggcggcg gaaccgccta gtccaagtaa caggcgcaaa tagagcgccg caatcgtaaa
     9001 ggttttgcct gtgccggcag aggcttcaat caggcgctcg ccctgtaagg gcaagcgcaa
     9061 aggatctagt gtctcggcga catcactcat tcttttcact catcaggggc attgtttgct
     9121 gcaacgcgct gacgttctcc cacactttcc agccttcagg atgcacatat tcggctttcc
     9181 cgttctggct gccggaaatc tgcgacagaa tagccatgcc ttgcggctcg accaccgcct
     9241 gatggaagaa atcagcaagt ttttgcggcg tcagcagttt tatctgggcc acgattttat
     9301 cacgcgaatc gaagcgcata ttgccgcgat cgaaatcttt acttaacttc gatgcttctt
     9361 cgccgagcgt ttgcggtgcc tgcagcatct gggtaattac cgcctgctgg atttgcgcaa
     9421 actcttctgg cttcatcgcc cgcaattttg cctctgcggt tgggaaaaac gccttgtaac
     9481 gctcccacaa gaaggaaggc tgtttatcat tgctttgcaa aaggaagccc atgccccact
     9541 gacgccccac gctcattgga aatgcaaaca cggcatagcc caattgttct tcggtacgca
     9601 actgattgta gaaccacggc tgtacgatct gccccaacag agagctatag gctgagctgg
     9661 tgtattcatc gtagccagtc ggtacaaata ccgctgccag tgcggagtcg gtgctgttac
     9721 cagctttttc aaagatgacg gattgttttt tatcgaccac gacatctttg ttacgacacc
     9781 actctgaacc atcagcgccc aactgttttt gcacatcgcg tgccagcgtt gttgcctggt
     9841 cctcggtcat gttgccgata accataaact ctggtcgagc ccctgatttt aaggcgtcgc
     9901 gataggccag cacctctttc aacgtaatgg agggcaaaat tttgcgccgt tcatctcgcg
     9961 agaagtacgg cacttgcgag agcatctgcg cgggcataat cgcctgctcg aacgctttgc
    10021 ccttttctgc ggaatccatc atctggttat accaggactt cgcctgctca agctgatctt
    10081 ccgtggcggt atagctaaag tagccttcca gcaatgcctg gaacagctgc ggcagatgct
    10141 gggtgtaacc attagcatta accataaggc cgttgttagc gttggtggaa aaacttatgc
    10201 caccaaccga cgcctggttg cttaactgat caagcgccag ccctgcgaga taatcattga
    10261 gcgcaaacat cacctgattg cgggcgctgt ccatggcttt cggattacgc aaaatcaggc
    10321 tgacatcggc ttttggctcg ctggcaaaat aacggcttgg cgcatacacc acgcgcagat
    10381 tcgactcatc aacaatcagc tctggatggt catatttctt gtctgactta atcagcgaga
    10441 aatcgtcagg aatataaggg ttaagctctg gcaaagagag cgcaatgtcg gcggcttttt
    10501 tctgccagtc ggcgaaagtt tgtgcgctga ttttatcgac ctgatacggc gcatcgacaa
    10561 agtaagccgt tttgttgtgc ggctctttcg ggctgatata ccagatacgc gcattctgcg
    10621 gcgtcatcat cgccagacgt tcctttactg ctttagcatc gtaccgatcg gcaatattga
    10681 ctgcatccag cgtatgctca acaggaacgc gaatcatggt atctgccagc cattcgacgt
    10741 aatccatatc acgggtgatt gacggataac ggaagtcgat atccagcaca ttcgccagtt
    10801 catcgaagta ttgtttatcg atcccttttt cacgtaacaa attgaggtag ctaaaaatag
    10861 ccgccacaac ctgatcgcga ttcgccagac ctttatcggt taaagacgca gagatcgcta
    10921 atacgccgct gttgccgttg acgataggat cggagttggc gctaatgccc tcaactaatc
    10981 cctgcttttg cagccagtcg gaaagtgtac ctgggctgcg attaccaatc agataggtaa
    11041 tcaattcatc ggttttgcta cggaatttcg ccgagttgtt atcaatacga aactctacgc
    11101 gcaacacttt acgcggcagc gccgggacgt aatgaatgat aatgcccttt tgcgcgtcgg
    11161 tgactaccgg cacggtgatt tccggttttt tgctctcttt gttcggcacg cgaccaaagg
    11221 tgtccgccgc catttttgcc aactccggca gcggtttatt gctgtaaata accgccttca
    11281 tcagattggc ggaatagtac ttctcgtgga aatctttcag cgcctgctgc accggattac
    11341 caggtttgtc gcttaaagtt tcgaggttac caccagaaaa ctttgaaccg gggtgtgccg
    11401 ggttaatggt ttctgcgctg acctgtgcca tgcgcatccc gtcacgcgta cgcgccatgg
    11461 ttaattcagc gttcactgca ttacgctcac gttcggcgta tttcttgtcg agcaagggtt
    11521 ctgcaatagc atcagccagg cggtctaccg caccaggcaa ggcgtcgttc tcaacttcca
    11581 gatagaaagc cgtgcgatac ggcgcagtgc tggcattgtg actaccgccg tgcattttga
    11641 gatattcggc cagactgtca gcctgcgggt actttttcga ccccatcaga ctcatatgtt
    11701 caaggtaatg tgccagcccc tggtacgcct cgggatcttc cagcgaccca acgggcacca
    11761 ccagcgccga gagcgattta actgcctgcg gatcagaaac cagcaagacc accataccgt
    11821 tatccagacg tatagcctga tactggcggt tatctttatc acttttacgg atggtttcct
    11881 gaatcggctg ccatcccgtt tctgcctgac ttaagggtgc ccaaagggca actaacaaca
    11941 ataatgcttt gaaccaggtg ctgcggggca ttcacggacc tcataatcaa cttaattttc
    12001 tgtccagatt caacacgtta acgcaaatca tctgccagaa tttaatcttg tgctgcacga
    12061 gtcagcctat gtttatataa ccatcagtcc gtgactggtg cgcatcataa agtaagcgga
    12121 tagattgcgc aatttttata cagcactcat gactgattaa agcgaaacag cggtaacagg
    12181 aaacgttgcg actgttcaac gatggcctcc attgtctctg gtgttaattg ccgccagagc
    12241 ctttgatacc agatatcatc accttcgcca cgcaccatca tgttgccttc gtaagcctga
    12301 aggaatttcg tacgggcttt ttgcaacgtg gaatcgtcat ccagcatggc atcgttttgc
    12361 gcgtcataac aggtttttag ccacgcgccg ccactttcag gtaacaccag caatggcgcg
    12421 gacatccctt cacgataccc ctcaatcagt tgtgagaggt aatgcaaagc ctgttcggct
    12481 gcaagcggcg gaaaacgcca ctcgccgtct ttgcgtagaa aaaggcgact ttcaccatta
    12541 ccaccgctgg cacagtagac aaggtgttcc agccaaagtt gcattccctg cgccacactt
    12601 aataaagagg gacgccagcg caacaggcca tccggctgca cctgcggcaa ccagccagtt
    12661 atctgcacac cgttgcaggc gagatcaatt tccatactct gccccggctg gcgacaggca
    12721 atgactctgt cggcaagctg ctgcatctcc tggcactgtg tttcccagaa aatttcacca
    12781 aaagcgccat acggtaaatc ccctgccgcc cggaagcggc ggaacaagcg ttcggcatca
    12841 tcctgctcaa ccagtgcatt caataactgc tgattgattt gataacggct aagcccttcc
    12901 agaataaatg gctcggtgtc tgggatttcg ctgtcttcag tacggaagtt cacctgcaaa
    12961 cgcatctgga agaacgcccg caccggatgt gcccagaatc gttgtagcgt ttccagcggc
    13021 acggtttccg gtaaggtaaa cggcagcggc tgaacaaatt cagaatgtgc tttaccagcc
    13081 tggctggccg caggtagcca ttcacgagca tagctttgtc gttcgcctgg ctggtagttt
    13141 tgcggatcaa acggcatccg ggtatggagg caagtaagat gcgcttttac ccttgcctcg
    13201 ctttcatcac agttgagcgc ttcatcgtcc ggtagataat gactttgccc gatgtagtcg
    13261 atcagttcct gcaccagtac cgacgggaaa cgttcactgt tatcctgaat ggaacgcccg
    13321 atatagctga tatagagttt ttgctgcgcg gaaattaacg cttccaggaa caggtagcgg
    13381 tcgtcatcgc gacggctacg gtcgccacgc ttcggtttct ggctcatcag gtcaaagccc
    13441 aatggcgcaa gctgacgtgg ataaacgccg tcgttcattc ccagcaggca aaccactttg
    13501 aacggaattg aacgcattgg catcagagta caaatgttaa ccggtccggc gagaaaacgc
    13561 tggctgatac gttcttgatc cagacgctgc gccagttcat cacgcaatag tgacagcggc
    13621 accgcgtcgc catactgcgc acctaaacct tcggcgataa tcgcctgcca ttgttgttcg
    13681 atcagcgtca tcgccgcttc ggtttccgca tccggcagga agaaggcgtt gagcatatcg
    13741 cgacaaaccg gcaaccactc ttctagcgga cgctcctgtg ccagcccgcg acgccagatg
    13801 tttagctgca ttagcagtga agccagatgc cccaccagtt ctgcaattaa gccgctcgat
    13861 tcatcataag gtagaaccga ttgccactcg ccctgcgcgc tctccatcgc gtagcccagc
    13921 aacatacgcg tcaggccaaa tcgccaggtg tgttgtccgg tggcggggag ttccagctcg
    13981 cgaacgttgt cgtcatctat cccccaacga atgccggatt cgttgaccca ctggcgtaaa
    14041 taacgcagcc cttcttcggt gatgtcaaac cgcgccgcca gcaccggcac atccagtaat
    14101 gccagcacgt cttccgacac aaagcggctg tctggcaacg ataacaggct gataaacgct
    14161 tccagtaccg gatgcgactg ccgcgcccga cggtcggaaa tggcgtaagg caggtaacga
    14221 tccgcaggtg cgctaccaaa cacagcctga ataaacggac tgtagctgtc gatatcagcc
    14281 accatcacga tgatgtcgcg cggagtaagt gtcgggtctt cttccagcat cgccagcagg
    14341 cgatcgtgca aaacttcaac ttcacgctgc gggctatggc aaacgtggaa ggtgatactg
    14401 ctatccagtg gatcaagcgg gcgtttgtta tcgctacggg aaaactcttc gatgttcaca
    14461 ccagcaacgg cacggttttc cagttccaga atgtcagact gaatgttatg cagcaggtta
    14521 tctggcgtga catcgacaaa ggcgtccagc tcctggctgc tctccaggtc agaaaggaga
    14581 taaatgtagt cgcgcccaag cttaccccat gaagccagca gcgggttgcc gacatcctgt
    14641 tcaccatcgc tgttaaagag ctgcccggca ttttcgctat cacgaaatag cggcaattcg
    14701 cgatcttcaa cactgtgtcg acgctgacgg gtcagcagtt tcgccagata agcaggatct
    14761 ttaatatcgc cccagtaata acggcagggg ttggtaaaca ggagatggat ttcaatatgt
    14821 ttacccagcg cctggagcgc ctggagataa acaggcggta acgcggaaat accgcatata
    14881 aagacgcgcg aaggtaaccc cggcgggcag gtcgtcgcgg actccagcgt ttcgataaag
    14941 cgctgataga gattggcgcg gtgccaacgc ggttgtccga gttcatgggt atattccacc
    15001 agcgccttcc acaacggcgc ttgccaggcc tgtgcttctc ccagcccttc aaccaaatgt
    15061 cctgtttccc actgtgccag ccactccggg cgatagacca gatactggtc aaacaggtcc
    15121 gccgcttttg aggaaagctg gaacagtttt cgcttgtcgc tatcgtcagc cagataatgc
    15181 cgcaacaggg taaagtcttc gcgatccagc aattgcggca gcagagtcat cagtttccag
    15241 ctcatgctct gtttgttaaa ggcgctctct ttggggatct ccggtaacac ccggacgaac
    15301 atatcccaga taaagctcgc tggcagcgga aaatcaatgt ttgccgcaat accaaacttt
    15361 tgcgacaggg tcatttgcag ccactgcgcc ataccggtac tttgcaccag aatcatctct
    15421 ggttcgaaag gatcgtccag ccgttcgcgt tcgacaataa actccatcaa cgcttccagc
    15481 acgtccagac gattggaatg gtagaccctt aacatagcgg ctcctgacta ctgacgattc
    15541 gggcaatgca ggcgggtcat ctcgccttct ctgcccccgg gtgaaactag cgtaacgctg
    15601 atgctgacac atcccgcctg cgatgtctgc attcggttga cctgccagtt ggcaggtggc
    15661 gaaatcgcgc gcagttgcgt ttgctgccaa ccatgccgcc agagctgttg gtactggttt
    15721 ctgctggcga gactgttcat taatgttcgc tggatacccg ataacgcagt gacaatcatc
    15781 accatcagca ccatcgcaac cattacctcc ggcaggctaa agccttgttg attcttcagg
    15841 gaagctgaca taacgccctc tctttcagcg gacaaaaatc gctccagccg cgtggcgaaa
    15901 aaacaatgtt tccatcaatg acttcgcctg ttcgccacaa cgaaacgcct tcataaccgg
    15961 caaccaataa ggcttcatta tctgccagta aacgcaaaca aacccgggca tcggttccag
    16021 cgtactgcga gcactgaact gcgggctgcg tctgccagga gtgcattttc ccccacgcca
    16081 gcgccgactg aacgatggcc tggcggcgca atgactgact ttccatgctc acgcgagagg
    16141 caaaactgcg atcctgctga ctcattcctt gcaatagcaa gctacccaaa accagcagca
    16201 tcaggaccag agccagtgac gaaacacctt tttcgcggtt cacaggttga atcctgtcac
    16261 gctatagctg gcatccacca cggtttgcgg ttcagactta ctggcagcac gcatattaac
    16321 cgtcaacacc ggcgagaagc cgctgacatc ctgacgtacg acctgaaaag tgtcgataat
    16381 gatggcatcc ggattagtga ctttatccca gcccttacct tcacaggatg tcgcaccgcg
    16441 tagcgtttcc agcacatgct ccttcagacg aaatccaatc tggtcagact cttttaccgg
    16501 ttcgcgatcc cagataccgt tactgttcgc atcccactgc acaatgatac agtcaccctg
    16561 tccgacaatt tccagccctt cgccggtaca gctgccatga caataacccg ccctctggag
    16621 atgcttcgcg acggtaaata cccgcagcca gatttcatct tccagcgcca gcttacgggt
    16681 gctcgttaaa ctttcacgct gtaacgcagg cagaaagcgt gcagccccca gcaacaatac
    16741 gctactgatc gccatagcaa tcaacacttc cagcagagaa aaaccttgct cttttacagg
    16801 catccttctg tttctccttg ctgacaaagc cggagtcttc cccacggcga aaccaccagc
    16861 caccactcgc ccgttgagtt tttgaagcga atatgcccgg cccatgcggt attgcgcagg
    16921 ccaaagaaag caagcgaagg tgtcaggtcg ctcatttcga cttcgggcca gcgtggcaca
    16981 aagaccaatg gtgaactgcc atgacaggta ttggccccag cagcggaact cacaaggcac
    17041 cataacgtcc cctccctgat aacgctgata ctgtggtcgc ggttatgcca gttggcatct
    17101 tcacgtaaat agagcaaata gtcccgcgcc tggctggcgg tttgccatag ccgttgcgac
    17161 tgctgccagt attgccagcc atagagtcca cttgcgctta gcatgaccaa aatcagcatc
    17221 gcgaccagcg tttcaatcag cgtataacca cgttgtgttt tcatgccggc agtatggagc
    17281 gaggagaaaa aaagacgagg gccagtttct atttcttcgg cgcatcttcc ggactattta
    17341 cgccgttgca ggacgttgca aaatttcggg aaggcgtctc gaagaattta acggagggta
    17401 aaaaaaccga cgcacactgg cgtcggctct ggcaggatgt ttcgtaatta gatagccacc
    17461 ggcgctttaa tgcccggatg tggatcgtag ccttcaatct caaagtcttc gaaacggtag
    17521 tcgaagatgg attcgggttt acgtttgata atcaacttcg gcagcggacg cggttcgcgg
    17581 cttaattgca gatgagtttg atccatatga ttgctgtaca ggtgcgtgtc gccccccgtc
    17641 cagacaaaat cgcccacttc cagatcacac tgctgcgcca tcatatgcac cagtaacgcg
    17701 tagctggcaa tgttaaacgg caggccgagg aaaacgtcac aggagcgctg ataaagctgg
    17761 caagagagtt tgccgtctgc cacatagaac tggaagaacg catggcacgg tgccagcgcc
    17821 attttatcca gttcgcctac gttccacgct gaaacaataa tgcggcgcga atccgggtcg
    17881 tttttcagct ggttcagtac cgtagtgatc tggtcaatat gacgaccatc cggcgttggc
    17941 caggcgcgcc actgtttacc atacactggc ccgaggtcgc cgttttcatc ggcccattcg
    18001 tcccagatgg tgacattgtt ttcgtgtaga taagcaatgt tagtgtcgcc ctgcagaaac
    18061 cacagcagtt catggatgat ggaacgcagg tggcaacgtt tagttgtcac cagcgggaat
    18121 ccatcctgca ggttaaaacg catctgatga ccaaaaatgg aaagcgttcc ggttccggta
    18181 cggtcgtttt tctgtgtgcc ttcgtcgagc actttttgca tcagttctaa atactgtttc
    18241 atggttcctc aggaaacgtg ttgctgtggg ctgcgacgat atgcccagac catcatgatc
    18301 acacccgcga caatcatcgg gatggaaaga atttgtccca tgctgatgta ctgcacccag
    18361 gcaccagtaa actgcgcgtc gggctggcgg aaaaactcaa caatgatgcg aaacgcgccg
    18421 taaccaatca ggaacaaacc tgagacagct cccattgggc gcggtttacg aatatacagg
    18481 ttgaggataa taaacagcac tacaccttcc agcaacagct cgtaaagctg tgatgggtgg
    18541 cgcggcagca caccgtaagt atcgaaaatg gattgccact gcgggttggt ttgcagcagc
    18601 aaaatatctt ctgtacggga gccagggaac agcatggcaa acgggaagtt cgggtcaacg
    18661 cggccccaca attcaccgtt aataaagttg cccagacgcc cggcaccaag accaaacgga
    18721 atgagtggtg cgataaaatc agagacctgg aagaaggaac gtttagtacg gcgggcgaag
    18781 ataatcatca ccacgataac gccaatcagg ccgccgtgga aggacatgcc gccgtcccag
    18841 acacggaaca gatacagcgg atcggccata aactgcggga agttgtagaa cagaacataa
    18901 ccaatacgtc caccgaggaa gacgccgagg aagcccgcat agagtaagtt ttcaacttca
    18961 tttttggtcc agccactgcc cggacgattc gcccgtcgtg ttgccagcca cattgcaaaa
    19021 atgaaaccca ccagatacat caggccgtac cagtgaagcg ccacgggtcc tattgagaaa
    19081 atgaccggat caaactccgg aaaatgcaga tagctactgg tcatctgtca ccacaagttc
    19141 ttgttatttc gctgaaagag aacagcgatt gaaatgcgcg ccgcaggttt caggcgctcc
    19201 aaaggtgcga ataatagcac aaggggacct ggctggttgc cggataccgt taaaagatat
    19261 gtatatgatc cgcgctataa ccctccgcga atcagcccgc ccatgccacg acgctccata
    19321 aaggctgcaa cctgatggcg aacttcggtc gccagttgcg cttccagact acgctgcgca
    19381 agattttctg cttcggcata atcaatgcgc cgcagcaggt attttacccg cgctacagaa
    19441 cgtccgttca tagacagatg gcgataccca agcccaatga ggattgccac gcacatggga
    19501 tcgcccgcca tttcaccgca caaacggaga tcgattccat gtatttccgc ttcccgggcg
    19561 atcatcgcca gagctcgtaa cattgcagga tgaagactgt cataaatgtt cgccacccgg
    19621 gtattgttgc gatcaacggc cagaatgtat tgagtcagat cgttggtgcc aacagagatg
    19681 aaatcgaccc gctttgccag atgcggcagc ataaatacca ttgacggcac ttccagcatg
    19741 atgccgatac gtggtttggg aatttcgtaa ccgatcatct cctcgacttc acatccggca
    19801 cgttcaatca ggcggcgtgc ttcatcaact tcatcgaggc ttgtgaccat cggcaacaga
    19861 atattcaggt tgcccgtagc ggcattagca cgcagcatcg cccgcacctg gatcaagaag
    19921 atctccggct gatcgagcgt aatgcgaatc ccacgccaac ccaggcatgg attctcttcg
    19981 ctgatcggca tgtaaggcag ctgcttatct gctccgacat ccagcgtacg caaggtgacg
    20041 ggtttatcat tgaacatttg cagcatcccc tgatactgcg ccacctgttc ttcttccgac
    20101 gggaaaccac tttgcagcat gaatgggatt tcagtgcgat aaagaccgat gccatcaata
    20161 cggctgccca gtttttcttc atgttccggg cttaaaccag cattgagcat gacttttata
    20221 cgctcaccgc tttttaactg cgcgggtaaa ttgacgtcat cttccgccag acggctaagc
    20281 tcaatctctt cactaattag ccgctgatat tcttgcagca gtaccggctc cggatcgacc
    20341 agcaattcac cgcgatagcc atcaacgatc agcgtcctac gatgcagcac cgaaggctga
    20401 atatccgcgc ccatcacggt agggatcccc agcgcacgta ccatgatcgc agcatgggag
    20461 ttggctgcgc catctcgcac gacaacaccg actaagcgat cctggggcag ctcagcaaga
    20521 gttgtcgctg acagttcatc tgccaccaga atgaaacgtt ccggccaggc gttcggccct
    20581 tgattagcgt catcaagatg aaacagcaat cgctgaccca gcgcacgtaa atcgccagcc
    20641 cgctctttga gatagttatc gcttagcgcg gcaaactgtt cggcaaattt ttcaatgacc
    20701 gtttttaccg cccactctgc caccgagcct ttatcaacct cggcaaacaa ttcgcgacgc
    20761 agccgggtgt ccgaaagtag gtgcgagtaa agatcgaaaa tagccgccgt ttctttttgt
    20821 gctccggcgg caaagcgttt gctgtagcgg cgaaactcgt ttgccgcttc ttccagcgcc
    20881 ccggtcagtc gttcgcgttc cagagccgga tccagcgttg atgcctgata cacctgttcc
    20941 attaaaggta acgtggcatc ctgccagcct tcggcaatcg ccacaccagg tgctgccggt
    21001 aatgcgcgga ttcgcgtctg gcgatattgc ccaaacaggg cagtcaactg cgactgggaa
    21061 agaatagctg ccatctgggt ggcaagcgtc accaggaagg attcttcact ttcgtcatac
    21121 tggcgcaact ctcgttgctg taccaccagt acaccaagca actggcgacg ttgaataatt
    21181 ggtacgccta aaaacgcgcg gaaacgttct tcttttacgg aggggatgta tttgaagctg
    21241 gggtgctttt gcgcatctgc aaggtttatc ggttccgcca gcctgccaac caggccgacg
    21301 atcccttcat caaacgcgag cgttacagtg cgaccgcgtg gttttttcag cccccgggtc
    21361 gccatcaggt agtaacaacg tcgatcatga tcggccaggt agaccgaaca gacctcggta
    21421 tccatcgcaa gacagatgtc ggtaaccaga atatttaacg cctcattcag gcgtggtgcg
    21481 ctggctacct tttcgactat ttcgcgcagg cgagtgagca taattggcgt gacttaacct
    21541 cttttacgtc gataagcaga tgcgttttgt ggttttggcg tattttcctg cagtgacatc
    21601 accacactcg cgaactcttt cattacccta cggtagacat cacgtttaaa tgacaccacc
    21661 tgtctgaccg gataccagta acttacccat cgccagccgt caaactctgg tgtactgctg
    21721 gtttgcatat tgatttctgc atcgccgctt accagctgca agagaaacca tttttgtttt
    21781 tggccgatac aaaccggctt cgtgtcccaa cgcaccaaac gtttcggtaa tttgtagcgc
    21841 aaccagttac gcgttgaagc aaggattcga acgtctttgc ggcttaatcc tacttcttca
    21901 aacaattcac ggtacatcgc ctgctctgcg gattctccgg ggttgattcc gccttgcgga
    21961 aattgccagg agtgctgacc aaatcgccgg gcccacatta cctgcccctg gcgattacaa
    22021 atcacgatac ctacgtttgg gcggtagcca tcgtcatcaa tcaccggact acctcaaaat
    22081 aaagctttat atacgaatga ttgtttcata ctccaggaag acggtaaacc actctctgca
    22141 gggcattaca cactaataac aattgaataa ctcacagtta tgtgcagagt tataaacaga
    22201 ggaaggggtg gatagccgtt tttattcacc ttttctgtgg atagagttgt gaagaactac
    22261 ggaattacta cgggaaaacc cggagcattc tgaataagcc cgatatagac attttaaaat
    22321 attcattatt agtcaatgca ttacatgtaa tttgagtaat gaaaaagagt aattcgtgac
    22381 ccagctcaca cctctcattt acgggttggc gaaagatcaa ccaatgccgt atttatccac
    22441 agaatgtgcc actaagttaa gcactgaacc actaaaaact ggagtttcgt cgcacgtcaa
    22501 ggctgtaaat ggaaacagta gtggaggttt ttcacagtta tcccagcttt ctgtggataa
    22561 catggtgtaa gatcctgttt attttcagtg accagatttg gaaaacctat tgcagtgttg
    22621 cgcaactcga ttaccggcag cctaaaaagc aatataaatc agagaattga acaacacata
    22681 tggaaaaagt tacactgcga atattcggca cataattgct gtttgttttt taaccaaggt
    22741 atcatgacat gtcccaacct cgcccactgc tctctcctcc cgaaactgaa gaacagttgt
    22801 tagcgcaagc acagcaactt tctggttata cattgggaga actggcggca cttgccgggc
    22861 tggttacgcc agagaattta aaacgcgata agggctggat tggcgtgtta ctggagatct
    22921 ggctaggtgc cagcgcaggg agtaaacctg agcaagattt tgctgctctg ggcgtggaac
    22981 ttaaaactat ccctgtggat agtcttggtc gtccgctgga aacaacattc gtttgtgttg
    23041 ccccgttaac gggtaatagc ggggtgacct gggaaaccag ccatgtgcgc cacaagctca
    23101 aacgcgtact gtggataccg gttgaaggcg agcgcagcat cccgctggcg cagcgtcgcg
    23161 taggatcacc gttgctgtgg agcccgaatg aagaggaaga ccgacagcta cgcgaagact
    23221 gggaagaatt aatggatatg attgttctcg gtcaggttga gcggatcacc gctcgtcacg
    23281 gggaatattt acagataaga ccgaaagcag cgaatgcgaa agcgcttacc gaagccattg
    23341 gtgcccgggg cgaacggatt ctgacgctgc cacgcggctt ttatttgaag aagaatttca
    23401 ccagtgcact actggcccgt cattttctga tccagtagcc atcgctttga cctgccgctt
    23461 tccgggcata taattaccgc ttcatttttt tggcagggct ttttagatgt tatttgcatg
    23521 gataaccgat cctaacgcct ggcttgcgct cggtacgctg acgctgctgg agatcgttct
    23581 tgggatcgac aatattattt tcctttctct ggtggtggca aagcttccca cagcacaacg
    23641 tgctcatgcg cgccgtctgg ggttggcggg agccatggtt atgcgtctgg cgctgctggc
    23701 atcaatcgcc tgggttacgc gcctgacaaa tccgcttttt acaatattca gtcaggaaat
    23761 ttctgcccgt gatttgattc tgcttctggg tggcttgttc cttatctgga aagccagcaa
    23821 ggaaatccac gaatccattg aaggtgaaga agaagggctg aaaacacgcg tttcatcatt
    23881 cctcggcgct atcgtgcaga ttatgctgct ggatattatc tttagcctcg actcggtgat
    23941 tacggctgtg ggtctgtcag atcacctgtt tattatgatg gcagccgtgg tgattgccgt
    24001 aggcgtgatg atgttcgccg cgcgctcgat tggtgatttt gtcgaacgcc atccttcggt
    24061 aaaaatgctg gcgctctctt tcctgattct ggtgggcttt accctgattc tggaaagttt
    24121 cgacatccac gtaccgaaag gttacatcta cttcgcgatg ttcttctcta ttgcggttga
    24181 aagcctcaac ttgattcgca acaaaaagaa tccgctctga tactccgtac gctctcctgc
    24241 gggagggcgt actcactcct tttaccctca cagtttaaga ttttcctgct ttcaagatat
    24301 ataacgtcgg tttataaaca gactattatc ataggtaggc caaatgagga taaccggatg
    24361 aaaaaatggg cagtaataat ttctgcagtc ggactggcgt ttgctgtttc cgggtgttcc
    24421 agtgattacg tcatggcgac caaagatggc cgtatgattt tgaccgatgg aaaacctgaa
    24481 attgatgatg ataccgggct ggtgagttat cacgatcagc aaggtaacgc gatgcaaatt
    24541 aaccgtgatg atgtttcgca aattattgaa cgttaacaaa taaggtcagc atccggctgg
    24601 ccttaagatt tttctctttc ccttttccct tccctctgcc atttttatat tccttatgtc
    24661 gtgattataa aaaggaaacg gctatgcaat atcaccgtat accccacagt tcgctggaag
    24721 tcagcacgct ggggcttggc acgatgacgt ttggtgaaca gaacagcgaa gccgacgccc
    24781 acgcacaact cgactatgcc gtcgctcagg gcattaacct tatcgacgtt gccgaaatgt
    24841 acccagtacc tccgcgcccc gaaacgcaag ggttaaccga aacctacgtc ggcaactggc
    24901 tggcgaaaca tggcagccgc gaaaagttaa ttatcgcctc caaagtgagc ggaccgtcgc
    24961 gcaataatga caagggcatc cgcccggatc aggcgctgga tcggaagaat atccgcgaag
    25021 cgctgcatga cagcctcaag cgcctacaga ctgattacct cgatctttat caggtgcact
    25081 ggccgcagcg cccgaccaac tgcttcggca aactcggtta tagctggacg gattctgcgc
    25141 ctgcggtttc gctgctggat acgctggacg cactggcaga gtaccaacgc gcgggaaaaa
    25201 ttcgttatat cggcgtgtcg aacgaaactg catttggcgt aatgcgctac ctgcatctgg
    25261 cggacaaaca cgatctgccg cgtattgtca ccattcagaa cccttacagt ctgttaaacc
    25321 gcagttttga agtaggtctg gcagaagtca gccagtatga aggggtcgaa ctgctggcct
    25381 attcgtgcct gggtttcggc acgctgaccg ggaaatatct caatggtgca aaacccgctg
    25441 gcgcacgtaa tacgctcttt agtcggttca cccgctatag cggtgagcaa acgcaaaaag
    25501 ccgtcgcggc gtatgttgat atcgccagac gtcatggcct ggaccctgct cagatggcgc
    25561 tcgcgtttgt acgccgtcaa ccgtttgttg ccagcactct gctgggcgca accacgatgg
    25621 atcagctgaa aactaacatc gaaagtttgc atctggagtt aagcgaagac gtattagctg
    25681 aaattgaagc ggtgcatcag gtttatactt atccggcacc ataagattaa cgccggttaa
    25741 aaccggcgtt aaatattaat gacggcgctg ccagatccac agcgccgtta ttgccagcgc
    25801 aaacagcgca ccgaagccaa tgccaatggg cacgaccggg atgcctatca ttaccgccag
    25861 cgagtaaatg cccagcatca acaacatggc gctgttttcg ccaaggtttt gtactgcaat
    25921 cgcattcccc gccccgacgc tttttttacc ccgctcctgt agcaacgcat tgagcggaac
    25981 gacaaaaaag ccccccatca cgccaatcag catcaacaag gcataggctg gcagcagctc
    26041 gtgttgcagg gaaaaaatca gtaccaccac gccaatcaaa atcccggctg gcatacagcg
    26101 tgacacggtt tccagcgtaa ctaacttcgc tgccgcacct gcgccaacca cgataccaat
    26161 cgctaccatc gcgttgagat aggtgggcgt agcgttatcg gtaatgccca gcgccaccgg
    26221 tacccacagc accaacagga aacgcagcgt gacacccgct ccccagaata aactggtgcc
    26281 caccagcgaa aaacgcgttt caccattgcg ccatagcgag gtgcaggcat tcaggaaact
    26341 gcgggtcatg ttgatgagat tccaggactg ccccggacgc gccgccgcca gtttgggaat
    26401 gtagatattg gcaacgaccg caccaccgta ggccagtgcg catgcggcca gggcgacgag
    26461 gacatgccag tcagccagca caccaccggc tacggaaccg agcaaaatcg ccgctatggt
    26521 agaagcttcc attaaaccgt tagctttcac taacttacta cccgtggtta attcgccgag
    26581 aataccgtat ttcgccggtg aataggctgc agcaccaaca cccaccagcg tatagccgag
    26641 aaacggattg ataccaaagc agatactggc tgcgcccagc agcttcaggc cgttggcaaa
    26701 catcatcacc cggcctttgg cgaagctatc cgccacctgc ccgacaaacg gcgcaaaaag
    26761 aatgtaagca cctacaaaca ccatttgcag gatgggctgg ctccactccg gatagaactg
    26821 cgctttcagt aacgccagag tggcaaacag tagggcatta tcgccaaacg cagagagaaa
    26881 ctgcgccacg ataaccgctt tcatcccctt cgaccacaac gaagtgttag tgtgcactga
    26941 ctcactcatc gtgttgttcc gcttcgtcta cccagctttt caacgtgaca aagtcaggtt
    27001 tgccgctgcc aagtaatggc atctgtttca gatagcgaat atcgcgcggt acagcaagct
    27061 ccggcacgcc gtgctcgcgg gcatactgtt gcaacttatc gcgcgtcagt tcgttatctg
    27121 tggtgaaaag caccagtgcc tcgcctttgc tggcatcgct cttaatcgca gtggcatgga
    27181 ctttatctgg cgaaacacca agtgccagtt gttccaccat ttccagcgac accatttcgc
    27241 ctgcaatttt ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa
    27301 aacgcacaat atcgccagtg tcataccagc cgcgctccat ttcgccgcga acattctcgg
    27361 cggtgggcac ttccagtaca cctggcttct ccacccgcag atagccgttc attatgttcg
    27421 gccctttcag ttgcaggcgt ccgccctctt cgataccagg aaccgacaac aggcgcgcat
    27481 ccattcctgg tagaatacgc cctaccgtac cgggtttcgc cgccatcggt acgttgatag
    27541 aaacgacagg cgcgcactcg gttacaccat agccttcaag gatgcgcagg ccaaatttat
    27601 cctgccaaag ctgtttggta ctttcttgta gtttttctgc gcctgccacc acatagcgta
    27661 gacgatagaa gtcatacggg ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac
    27721 cgaacaacac ggtgcaactg cggtcataca ccaactccgg cacaatgcgg taatgcagcg
    27781 ggcttggata aaggaacact tctgcacctg taagcagtgg cgtaaacagg cctaccgtca
    27841 gcccaaagga gtgaaacagc ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg
    27901 caatcgtttt aatctgctcg acgttcgcca gaatgctttt atggctatgg acgacgcctt
    27961 ttggatggcc ttcagaaccg gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt
    28021 ttacctgcgc caggcgcggc atcagcaaat gagcgaagat ccatactttg tcggcagtgg
    28081 tgacatctgc ttttaaatct tccagataga cccagcgcac ctgagtaagt tgctccggca
    28141 gatgccagag tttgccttta tcgagaaact ggcgggaagt gaagatggtt ttgatttcag
    28201 ccgccgtaat agcactggtc agccctttta ccccagcagt gtagttcatc attgcgggaa
    28261 tgcggcgacg cgcgatggcc ccaaaaatca ctgccgcact gatgcctgca ttgggcagca
    28321 ttaagccgat gcgttcgcct tcaacactgt atttttcaag gatgcgtcca acaaacagcg
    28381 ttttcgtaag caatttgcga taggagtctg gggtaaagtt gacgtcttcg acacatttct
    28441 tcccggctcc gaagcggtac attgcactca gtaaagattc gtacagagtt tcacgcgggc
    28501 gcaccgccat acgcgcttcc atcattattt gatgcagcat ttcgccagcg attttgcgac
    28561 ggtcacgggc acgcggcgca tccggcatcg ccacctgcgt tggtggcaaa atatgcagag
    28621 taatttgcgg gaacaagcga cgtttaacca gacctttcag gcggctgaag tgcgtaagtt
    28681 ccgccccttc aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg
    28741 cgccatcgta gattttcatc agcgagcctg tagtggtgat gcgtccttca gggaaaatca
    28801 ccactggtcg gccctgttcc accagacgta ccagatgttt aatagccata ggttgcgtcg
    28861 ggtcgagagg aacaaagtcg ataaatgatt tcagccaacg catataccac tgttggctta
    28921 ttgaggtgta aacggcaaac actggacgca caggtaaaaa cagtccaagc aaaatgccat
    28981 caataaaaga gacgtgatta ggcgtaatta gaacgcgctc gcccttcagt gcctgggtgt
    29041 cacccgtaac gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca
    29101 ttccaactcc ctttgctctg attcagtaaa agcgaatgga gggagattac acgagataaa
    29161 gaacgcgagc gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg
    29221 caagagacag ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc tctgggatct
    29281 tgattgtggt ctgcacgacg actcttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa
    29341 tgcaacgagg tgtgtaaatt gtcggttact gttacagatt gatgaccggc aaaaaaaaac
    29401 ctgcgcatct gcgcaggctg gtgtaattca tgtgctcaac ccgaagttga cttcacctat
    29461 caatacctct gggatcacca ctttagcaac ctgaagccaa acgccaccag cggtcaatcg
    29521 caacagcgtt tcgcaaagtg taaccaaagg tttgcaatct ctttttttgt cttacttatt
    29581 tgcgtttggc tcgcattccc acgatgaaaa cacgccaccc cttgaaccaa cgggcgtttt
    29641 ccgtaacact gaaagaatgt aagcgtttac ctactaaggt attttcatgg cgaccataaa
    29701 ggatgtagcc cgactggcag gcgtttcagt cgccaccgtt tcccgcgtca ttaataattc
    29761 acctaaagcc agtgaagctt cccggctggc tgtgcatagt gcaatggagt ctcttagcta
    29821 tcacccgaac gccaacgccc gtgcgctggc gcagcagacc actgaaacgg tcggtctgat
    29881 cgttggtgat gtttccgatc cgtttttcgg tgcaatggtg aaagcggtcg aacaggtggc
    29941 ttatcacacc ggtaatttct tattgattgg caacggttac cacaacgaac aaaaagagcg
    30001 tcaggctatt gagcaactga tccgccatcg ctgtgctgcg ttggtcgtcc atgccaaaat
    30061 gatcccggat gccgatttaa cctcattaat gaaacaaatg cccggtatgg tgctgatcaa
    30121 ccgtatcctg cctggctttg aaaaccgttg tattgctctt gacgatcgtt acggtgcctg
    30181 gctggcaacg cgtcatttaa ttcagcaagg tcatacccgc attggttatc tgtgctctaa
    30241 ccactctatt tctgacgccg aagatcgtct gcaagggtat tacgatgccc ttgctgaaag
    30301 tggtattgcg gccaatgacc ggctggtgac atttggcgaa ccagacgaaa gcggcggcga
    30361 acaggcaatg accgagcttt tgggacgagg aagaaatttc actgcggtag cctgttataa
    30421 cgattcaatg gcggcgggtg cgatgggcgt tctcaatgat aatggtattg atgtaccggg
    30481 tgagatttcg ttaattggct ttgatgatgt gctggtgtca cgctatgtgc gtccgcgcct
    30541 gaccaccgtg cgttacccaa tcgtgacgat ggcgacccag gctgccgaac tggctttggc
    30601 gctggcggat aatcgccctc tcccggaaat cactaatgtc tttagtccga cgctggtacg
    30661 tcgtcattca gtgtcaactc cgtcgctgga ggcaagtcat catgcaacca gcgactaacc
    30721 gcagttaaag caattccagc gccagtaatt cttcgatggt ctggcgacgg cgaatcaacc
    30781 gcgcctgacc attatcaaac agaacttctg gtaacagcgg acggctattg tagttggatg
    30841 acattgatgc gccatatgcc cctgtatcat gcagtaccag ataatcaccc gccttcactt
    30901 ccggcaaggc gcgggtttca acatttcccc cttcctgctg ggtaaagaca tcgcccgatt
    30961 cacataatgg cccggcgacg acggtttcca ccgttggcgc gtgttccaga gaacgaccat
    31021 cagctgccag ggcactgata tggtggtaac taccgtacat tgccgggcgc atcagatcgt
    31081 tgaacccggc atcaaccagc acaaagtggc ggctacccat ttgtttgacg ctccgcacct
    31141 gagtaattaa cacgccagac tgcgctacca gaaagcgacc cggttcaatt tccagtttca
    31201 cagggtggcc caaatggcga gcgatttgct cacgcgcggc attccacaga ccataataat
    31261 gttcggtatc aaccgcctct tcaccctgtt gataaggaat agaaagccca ccgcccgcag
    31321 aaatagcctg taaatcctga ccgaattcga tgacctgacg taccatagca ccacacacct
    31381 gttccagatg ggcataatca acgccagaac caatgtgcat gtgaatgccg accagctgca
    31441 aatgatgacg ttgtatcacg tccagtgcgg cgggcagatc ggtgtaccag ataccgtgct
    31501 tgctgttttc gccaccggta ttggtttttt ggctatgtcc gtgaccaaac cccgggttaa
    31561 cgcgcagcca tacccgatgc cctggcgaaa cctggcccag ttggtcgagc atatcaacag
    31621 aacccgcatt caccggaatt tgcaattcac tgacgcgttc aagcgtcgcc tgatcgataa
    31681 catctgccgt aaaaacaata tcatcggggt gcgtttgcgg attgtaaccc gccgccaacg
    31741 cacgctctat ttcgcctaac gagacggaat ccactttcac gccctgctca cgcattaatc
    31801 gcaaaatatg aatattggaa caggctttct gtgcaaagcg caccacatca aactgtttca
    31861 gcgctgcaat ctgccgacga ataatttgcg catcgtagac ccacaccggg cagccaaatt
    31921 cagcgggcaa acgcagcaga ttttcggcgg tgagatcggt atcggtgctg aacagtgaat
    31981 gtggcataac aaactccaga tgagtgcttt tttatgatta cgccacatca taaaaagaat
    32041 aaaaaatatc gatttatgtc gagtctatgc aaaaatgata tggattaccg gattgcgaga
    32101 gagcgctaat ggccgccgtt aacttacgtc atattgaaat ttttcatgcg gtaatgaccg
    32161 ccggaagcct gactgaggca gcacacctgc tacacacctc acagccaacc gtcagccgcg
    32221 aactggcgcg ctttgagaag gtgatcgggc taaaattgtt tgagcgcgta cgtggacgat
    32281 tacatcctac cgtgcaagga ctgcgtctgt ttgaagaagt gcaacgatcc tggtacggac
    32341 tggatcgcat tgtcagcgcc gcagaaagtc tgcgcgagtt tcgccaggga gaactgtcta
    32401 ttgcctgcct gccggtcttt tcgcaatctt ttttaccgca gctcctgcaa ccctttctgg
    32461 cacgttatcc cgatgtcagc ttaaatatcg tgccccagga atcaccgcta cttgaagagt
    32521 ggctctcggc ccagcgtcat gatttaggac tcactgagac actccatacg cctgcgggaa
    32581 cagaacgtac cgaattactc tctttagatg aagtgtgtgt gttacctccg ggtcatccgc
    32641 tggcggtaaa aaaggtatta acgccggatg attttcaggg tgagaactac atcagccttt
    32701 cccgtactga cagctatcgc cagttgctgg atcagctatt tactgaacat caggttaaac
    32761 gacgcatgat cgtagaaacc cacagcgccg cgtcagtctg cgcaatggta cgggcggggg
    32821 taggtgtttc ggtggttaac ccgctcaccg cactggatta tgcggcaagc ggtttagtgg
    32881 tacggcggtt cagtattgcg gttccgttca ccgtcagcct gatccgcccc ctgcaccgcc
    32941 cgtcatcagc gctggttcag gcgtttagtg agcatttaca agcggggtta ccgaaactgg
    33001 tcacttctct tgacgcgatt ttgtcgtcag ctacgacagc ataaaagcga cagcatcctc
    33061 ggcatggatc gccgcagtat caaacacagg caaaacactg cgctcttctg gaaccaataa
    33121 accaatttct gtgcagccaa aaatgacacc ctgcgccccc tgttctgcaa ggcgagcaat
    33181 cacttgcgca taataagcgc gtgacgcttc ggtaaattgc cccagacaca gttcttcaaa
    33241 aataatctga ttaattttcg cccgttcatc cgcttcagga ataagacaat tgatggaaaa
    33301 ttgttccgtc agccgcccgc gataaaaatc ctgttccatg gtgtaacgcg tacccagcag
    33361 cgccacacga gtcattccgg ccccggtaat tgcacgtccg gtggcatccg caatgtgtaa
    33421 gaaaggcaga gtgcaacgtg actcaatggc atccgccact ttatgcatcg tattggtaca
    33481 taacacaata ccttctgcgc ccgcccgctg taagccaagc gccgcctcag ccagaatgtc
    33541 cccggtttta tcccattccc cgcgacgctg gcactcttct atttcatgaa aatcgacgct
    33601 atgtagcagc acttgcgcag agtgaagccc accaagccgc tgtttaatgc cttcatttat
    33661 cagacgatag taaggaatgg tggattccca gctcattcct cccagcaaac caattgtttt
    33721 cattcctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg agacaaacgc
    33781 ctcaacggcc aggtgcccga tctctattaa cgaaaaaagg gccggatgta cagcacatcc
    33841 ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag tttgcgttcg
    33901 atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtgat gcccacaaac
    33961 gcaatgttca gcgcagtgta gagccagaac gtaccggcag cgccaatgct atcaagcagt
    34021 gtcaggaagg tcgcgccgat aatcatattc gacacccagt ttgtcgtcgt cgaacaggta
    34081 ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca caccactggc
    34141 gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga gagccaggac
    34201 aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag caccagagtg
    34261 cctaacgcca tcacgctgaa accaattttc agagccggtt tacgacctgc tttatctacc
    34321 gtaaagaccg caatgaaggt cgcgaacata aaggtcagtc ccacgaccag agtcgcaatc
    34381 atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc atagtacatg
    34441 atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag aaacacagca
    34501 cgacggacgt tacggttgat cttaaacagt gcccaaccgc cttgttttaa cttcaggctt
    34561 tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag catacgcaac
    34621 acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct atttggcagg
    34681 aagaccacca gaataatcag caggactgct ggtaaagcaa gaacccccaa cattgcgcgc
    34741 cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat gccgagtgtg
    34801 accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc catttcagaa
    34861 agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac cacacgagcg
    34921 gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag tacaaacagg
    34981 atggcccccg ccatcaggct gtatttacga cccaggcgga acgacagcca accattaaac
    35041 agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg caaacgactg
    35101 gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc gatatcaaga
    35161 ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat acgccgcgta
    35221 tcccgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat tttttcctgc
    35281 cagcagagag taagacatag tgaaaaaata cgtgaacaac tcacgcaggt gtcaggtcgg
    35341 aaacagcata aatatggatt aaattgctgc gacatgtcgt tatgtgatgg atattccaat
    35401 tttcaaatta agttgaatta ttgagattat tattaaccac ctaattttac agcagataaa
    35461 attcataaag ttcattaatt gataattaat atggattatt tcataaccat gatatggatt
    35521 atgatgatct acaggtataa aaaaccctgc catgcggcag ggtcataaaa gtaagaagaa
    35581 tgaattaacg cgccagccaa ccgccatcca cggcaatggt ataaccattc acataatctg
    35641 aagcgctgga ggcaaggaac actatcggcc ccatcaggtc actcggcagt ccccaacgac
    35701 cagctggaat gcggtcgaga atttccgcgc tacgttgttc atctgcccgt agttgttgag
    35761 tattgttggt cgccatgtaa cccggagcta tcgcattaac attaatgttg tgtttagccc
    35821 attcgttcgc catcaatcgc gtcacaccca tcacgccgct ttttgatgcg gtataagaag
    35881 gcacacggat cccgccctgg aaggagagca ttgacgcgat attgataatc ttgccgccat
    35941 tgccttgcgc gataaagtgt ttcgccgctg cctgagacat gaagaatacg ctcttgatat
    36001 tcaggttcat gacatcgtcc cagtcctttt cgctgaactc gagagcatct tcgcggcgaa
    36061 tcaatccggc gttattcacc aggatatcaa tatgaccaaa ctccgctacc gcgcgatcca
    36121 gcagtccagg aatgccatca atctttcgca gatcggcggt caggcttaaa aaacgacgcc
    36181 ccagcgccgt gacctgctcg atggtttcag tcggttcaac gatgttaatg ccaacaatgt
    36241 cacagcccgc ttgcgccagc cccaacgcca tcccctggcc cagcccagta tcacaaccag
    36301 tgacgaccgc aactttacct tcgagagaaa atgcacttaa aatcataaca ataccttatt
    36361 cgtttatgcc cacaactagc gcaaatcttt aacggccaca tggtccatat catcaaagac
    36421 ctggttttca ccgaccatgc cccagataaa ggtataagct ttggttccga cgccggaatg
    36481 aatcgaccag ctcggggaga tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc
    36541 ttgcggctgc cccatcatgt ggaaaacgca ggcgtcatca tccatattga aatagaaata
    36601 aacttccatc cggcgctcgt gagtgtgaca cggcatggtg ttccacaagt tacctggagc
    36661 cagctccgtc agccccatac tcaattggca ggtttccagc acatccggga caaaatattt
    36721 gttaattgtg cgacggttac tggtgaggtt atcgcctaac gtgactggag atacttcgtc
    36781 cggtgtgact tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt
    36841 tgccggagtg gcggtatcaa tactggcaaa gacaacttct tttgcacctt tacccacata
    36901 cagggcgtcg cggtgaccga tttcatagca ttggccatcg acagtaatcg taccggcacc
    36961 gccaatattg ataacaccta actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt
    37021 accaacttcc ccgccaacgg aaaccgtttt agttatcggc ataatgccgc caacaataat
    37081 tcggtcaatg tggctgtaaa ccatggtgta ctcatcggcg acaaatacct cttcaaccaa
    37141 aaattcattg cgcagccctt gggtatccag cgtttttgcg tgcgcactgt ggatgctctg
    37201 tcttacgtcc acatcaacct ccgataatca gtgaagcgtg aaaatagaaa acgatagtga
    37261 aacactgttt cgttttctct ataaagggat gatatgccca tgggctaggg ttttcaatta
    37321 aaaataaaac aatgtttcat ttttaagtta agaattaaaa aaagtgcagt gtcgatcacg
    37381 aatatgtcat gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc
    37441 atcattattc aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct
    37501 actcgtcacg ttcaatggtc aatgccacgc cctggccccc gccgatacaa agcgttgcca
    37561 gccctttgcg ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac
    37621 cggaagcgcc tatcgggtga ccgagtgcga tcgcgccacc attgacattg acccgacgct
    37681 catcccactc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct
    37741 cgataagatc gacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat
    37801 acaccggcgc aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcggatgc
    37861 gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag
    37921 ctgcgccatc gtttatggat gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg
    37981 aaggatttaa acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat
    38041 caacaaccaa cgtctgaccg ttactttgga tcattaccgg gacgatctca tctttaaatc
    38101 gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca
    38161 actgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat
    38221 cattgaaggc atcccacaac ccatcatgca ccagactgtc aaccaactgg ctattgccaa
    38281 gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc
    38341 caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg
    38401 ctttaagccc agaaccgcaa acgtcattaa tagtgattgc agaaacgcta ttaggaagac
    38461 cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac
    38521 caagaattac ttcatccacc gcatatgcag gaacgccggt acgttctatt aacgctttca
    38581 cgaccagact accaagttcc acggcggaat gacccgctaa cgcaccgcga aagcagccga
    38641 taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa
    38701 cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa aaagtgattt
    38761 ttatcacaaa ggaaatatgc ctgagcagta gtcagagtca taactggcac gtaaggtttg
    38821 caaccactaa cccaccaata gaggggtaga tagggcgtta atctcccata cttaacctgg
    38881 tttatggtaa attgccctcc attttgttta atttgtagat gatacgttca gataatgtct
    38941 aatatttggt ctaaagaaga aactctgtgg agtttcgcgc tctacggcac agccgttggt
    39001 gcaggcacgc tcttccttcc tattcagtta ggttcggcag gggctgtggt cctgtttatt
    39061 actgctctgg tcgcctggcc tttaacatat tggccacata aagccttatg ccagttcatc
    39121 ctctcatcga aaacatcagc aggtgaaggg ataacgggcg cagtaacaca ctactatggc
    39181 aagaagattg gtaatctgat taccacgctg tacttcatcg ccttttttgt cgtcgtgttg
    39241 atatatgcag tggcaattac caactcactt acggaacagc tggcaaagca tatggttatt
    39301 gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat taatcctgaa tctcattttt
    39361 ctgatgggac gccatgcaac cattcgggta atgggttttt tggtattccc attgattgcc
    39421 tatttcttat ttctttccat ttaccttgtc ggtagttggc aacctgatct attaacgacc
    39481 caggtagagt tcaatcagaa tacccttcac cagatatgga tatcgattcc cgtgatggtt
    39541 ttcgccttta gccatacgcc cattatttct acgtttgcca tagacagacg tgaaaaatat
    39601 ggcgaacacg ctatggataa atgcaaaaaa attatgaaag tcgcttatct catcatctgc
    39661 ataagtgtac tgttctttgt ctttagctgc ctgctttcta ttccaccttc gtatattgaa
    39721 gcggctaaag aagaaggggt caccatttta tcggcgcttt ctatgctgcc gaacgcccca
    39781 gcatggctgt caatttccgg gattattgtc gcagtagttg cgatgtcgaa atcattcctg
    39841 ggtacgtact ttggcgttat tgaaggtgcc acggaggtcg tcaaaacaac actacagcag
    39901 gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat caattatgtt ggtatcgctg
    39961 atcaccttca ttgtttgttg cattaacccg aacgcgattt cgatgattta cgcgatcagc
    40021 ggcccgctca ttgccatgat acttttcatc atgcctacgt tgtcaacgta tctgatcccg
    40081 gcgcttaaac cctggcgttc catcggaaat ctgattacgc tgatcgtggg tatcctgtgc
    40141 gtatcggtaa tgttctttag ctgatatcca ttgtgaatgg ttcaaaattg ctacaatttt
    40201 gaaccattcc agttttcata atgttcattc gttattattc cgcactatca cttccctcat
    40261 catatcctag tattatcagt tagttaaaca cacaaagaga tctgaacatc ttaaataaga
    40321 ctatttaaga tgcataactt agattcgcaa gatatcttcc ggtcattcaa aaacaatttg
    40381 cgatatagat attctcatac catgagatag ttatctgaac caccc
//
LOCUS       DAZFWL020000026        40192 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFWL020000026 DAZFWL020000000
VERSION     DAZFWL020000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.026"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..198)
                     /gene="yiiE"
                     /locus_tag="ACHKNP_RS07575"
                     /old_locus_tag="pgaptmp_001515"
     CDS             complement(<1..198)
                     /gene="yiiE"
                     /locus_tag="ACHKNP_RS07575"
                     /old_locus_tag="pgaptmp_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGU4703710.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQ"
     gene            complement(803..1093)
                     /gene="nadS"
                     /locus_tag="ACHKNP_RS07580"
                     /old_locus_tag="pgaptmp_001516"
     CDS             complement(803..1093)
                     /gene="nadS"
                     /locus_tag="ACHKNP_RS07580"
                     /old_locus_tag="pgaptmp_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HGU4703711.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1094..1405)
                     /locus_tag="ACHKNP_RS07585"
                     /old_locus_tag="pgaptmp_001517"
     CDS             complement(1094..1405)
                     /locus_tag="ACHKNP_RS07585"
                     /old_locus_tag="pgaptmp_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4703712.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1634..2542
                     /locus_tag="ACHKNP_RS07590"
                     /old_locus_tag="pgaptmp_001518"
     CDS             1634..2542
                     /locus_tag="ACHKNP_RS07590"
                     /old_locus_tag="pgaptmp_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4703713.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2606..3547)
                     /gene="fabY"
                     /locus_tag="ACHKNP_RS07595"
                     /old_locus_tag="pgaptmp_001519"
                     /gene_synonym="yiiD"
     CDS             complement(2606..3547)
                     /gene="fabY"
                     /locus_tag="ACHKNP_RS07595"
                     /old_locus_tag="pgaptmp_001519"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HGU4703714.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3592..4029)
                     /gene="dtd"
                     /locus_tag="ACHKNP_RS07600"
                     /old_locus_tag="pgaptmp_001520"
     CDS             complement(3592..4029)
                     /gene="dtd"
                     /locus_tag="ACHKNP_RS07600"
                     /old_locus_tag="pgaptmp_001520"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGU4703715.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4026..4898)
                     /gene="yihY"
                     /locus_tag="ACHKNP_RS07605"
                     /old_locus_tag="pgaptmp_001521"
     CDS             complement(4026..4898)
                     /gene="yihY"
                     /locus_tag="ACHKNP_RS07605"
                     /old_locus_tag="pgaptmp_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HGU4703716.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4892..5491)
                     /gene="yihX"
                     /locus_tag="ACHKNP_RS07610"
                     /old_locus_tag="pgaptmp_001522"
     CDS             complement(4892..5491)
                     /gene="yihX"
                     /locus_tag="ACHKNP_RS07610"
                     /old_locus_tag="pgaptmp_001522"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HGU4703717.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5590..6375)
                     /gene="csqR"
                     /locus_tag="ACHKNP_RS07615"
                     /old_locus_tag="pgaptmp_001523"
     CDS             complement(5590..6375)
                     /gene="csqR"
                     /locus_tag="ACHKNP_RS07615"
                     /old_locus_tag="pgaptmp_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4703718.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(6409..7305)
                     /gene="yihV"
                     /locus_tag="ACHKNP_RS07620"
                     /old_locus_tag="pgaptmp_001524"
     CDS             complement(6409..7305)
                     /gene="yihV"
                     /locus_tag="ACHKNP_RS07620"
                     /old_locus_tag="pgaptmp_001524"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HGU4703719.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            7473..8369
                     /gene="yihU"
                     /locus_tag="ACHKNP_RS07625"
                     /old_locus_tag="pgaptmp_001525"
     CDS             7473..8369
                     /gene="yihU"
                     /locus_tag="ACHKNP_RS07625"
                     /old_locus_tag="pgaptmp_001525"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HGU4703720.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            8393..9271
                     /gene="yihT"
                     /locus_tag="ACHKNP_RS07630"
                     /old_locus_tag="pgaptmp_001526"
     CDS             8393..9271
                     /gene="yihT"
                     /locus_tag="ACHKNP_RS07630"
                     /old_locus_tag="pgaptmp_001526"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HGU4703721.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            9288..10529
                     /gene="yihS"
                     /locus_tag="ACHKNP_RS07635"
                     /old_locus_tag="pgaptmp_001527"
     CDS             9288..10529
                     /gene="yihS"
                     /locus_tag="ACHKNP_RS07635"
                     /old_locus_tag="pgaptmp_001527"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HGU4703722.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            10667..11569
                     /gene="yihR"
                     /locus_tag="ACHKNP_RS07640"
                     /old_locus_tag="pgaptmp_001528"
     CDS             10667..11569
                     /gene="yihR"
                     /locus_tag="ACHKNP_RS07640"
                     /old_locus_tag="pgaptmp_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HGU4703723.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            11768..13804
                     /gene="yihQ"
                     /locus_tag="ACHKNP_RS07645"
                     /old_locus_tag="pgaptmp_001529"
     CDS             11768..13804
                     /gene="yihQ"
                     /locus_tag="ACHKNP_RS07645"
                     /old_locus_tag="pgaptmp_001529"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HGU4703724.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDNDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            13850..15235
                     /gene="yihP"
                     /locus_tag="ACHKNP_RS07650"
                     /old_locus_tag="pgaptmp_001530"
     CDS             13850..15235
                     /gene="yihP"
                     /locus_tag="ACHKNP_RS07650"
                     /old_locus_tag="pgaptmp_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4703725.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            15278..16681
                     /gene="yihO"
                     /locus_tag="ACHKNP_RS07655"
                     /old_locus_tag="pgaptmp_001531"
     CDS             15278..16681
                     /gene="yihO"
                     /locus_tag="ACHKNP_RS07655"
                     /old_locus_tag="pgaptmp_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4703726.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            16749..17441
                     /gene="ompL"
                     /locus_tag="ACHKNP_RS07660"
                     /old_locus_tag="pgaptmp_001532"
     CDS             16749..17441
                     /gene="ompL"
                     /locus_tag="ACHKNP_RS07660"
                     /old_locus_tag="pgaptmp_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HGU4703727.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(17532..18797)
                     /gene="yihN"
                     /locus_tag="ACHKNP_RS07665"
                     /old_locus_tag="pgaptmp_001533"
     CDS             complement(17532..18797)
                     /gene="yihN"
                     /locus_tag="ACHKNP_RS07665"
                     /old_locus_tag="pgaptmp_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4703728.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(18899..19879)
                     /gene="yihM"
                     /locus_tag="ACHKNP_RS07670"
                     /old_locus_tag="pgaptmp_001534"
     CDS             complement(18899..19879)
                     /gene="yihM"
                     /locus_tag="ACHKNP_RS07670"
                     /old_locus_tag="pgaptmp_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGU4703729.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(19887..20597)
                     /gene="yihL"
                     /locus_tag="ACHKNP_RS07675"
                     /old_locus_tag="pgaptmp_001535"
     CDS             complement(19887..20597)
                     /gene="yihL"
                     /locus_tag="ACHKNP_RS07675"
                     /old_locus_tag="pgaptmp_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4703730.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(20814..22637)
                     /gene="typA"
                     /locus_tag="ACHKNP_RS07680"
                     /old_locus_tag="pgaptmp_001536"
     CDS             complement(20814..22637)
                     /gene="typA"
                     /locus_tag="ACHKNP_RS07680"
                     /old_locus_tag="pgaptmp_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HGU4703731.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            23010..24419
                     /gene="glnA"
                     /locus_tag="ACHKNP_RS07685"
                     /old_locus_tag="pgaptmp_001537"
     CDS             23010..24419
                     /gene="glnA"
                     /locus_tag="ACHKNP_RS07685"
                     /old_locus_tag="pgaptmp_001537"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HGU4703732.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            24738..25787
                     /gene="glnL"
                     /locus_tag="ACHKNP_RS07690"
                     /old_locus_tag="pgaptmp_001538"
     CDS             24738..25787
                     /gene="glnL"
                     /locus_tag="ACHKNP_RS07690"
                     /old_locus_tag="pgaptmp_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HGU4703733.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            25799..27208
                     /gene="glnG"
                     /locus_tag="ACHKNP_RS07695"
                     /old_locus_tag="pgaptmp_001539"
     CDS             25799..27208
                     /gene="glnG"
                     /locus_tag="ACHKNP_RS07695"
                     /old_locus_tag="pgaptmp_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HGU4703734.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            27320..27430
                     /gene="yshB"
                     /locus_tag="ACHKNP_RS07700"
                     /old_locus_tag="pgaptmp_001540"
     CDS             27320..27430
                     /gene="yshB"
                     /locus_tag="ACHKNP_RS07700"
                     /old_locus_tag="pgaptmp_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HGU4703735.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(27620..28993)
                     /gene="hemN"
                     /locus_tag="ACHKNP_RS07705"
                     /old_locus_tag="pgaptmp_001541"
     CDS             complement(27620..28993)
                     /gene="hemN"
                     /locus_tag="ACHKNP_RS07705"
                     /old_locus_tag="pgaptmp_001541"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HGU4703736.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKLW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(29182..29691)
                     /gene="yihI"
                     /locus_tag="ACHKNP_RS07710"
                     /old_locus_tag="pgaptmp_001542"
     CDS             complement(29182..29691)
                     /gene="yihI"
                     /locus_tag="ACHKNP_RS07710"
                     /old_locus_tag="pgaptmp_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HGU4703737.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(29810..29976)
                     /locus_tag="ACHKNP_RS07715"
                     /old_locus_tag="pgaptmp_001543"
                     /pseudo
     CDS             complement(29810..29976)
                     /locus_tag="ACHKNP_RS07715"
                     /old_locus_tag="pgaptmp_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            30273..30905
                     /gene="yihA"
                     /locus_tag="ACHKNP_RS07720"
                     /old_locus_tag="pgaptmp_001544"
     CDS             30273..30905
                     /gene="yihA"
                     /locus_tag="ACHKNP_RS07720"
                     /old_locus_tag="pgaptmp_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGU4703738.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(31287..34073)
                     /gene="polA"
                     /locus_tag="ACHKNP_RS07725"
                     /old_locus_tag="pgaptmp_001545"
     CDS             complement(31287..34073)
                     /gene="polA"
                     /locus_tag="ACHKNP_RS07725"
                     /old_locus_tag="pgaptmp_001545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGU4703739.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFSLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            34437..35369
                     /gene="yihG"
                     /locus_tag="ACHKNP_RS07730"
                     /old_locus_tag="pgaptmp_001546"
     CDS             34437..35369
                     /gene="yihG"
                     /locus_tag="ACHKNP_RS07730"
                     /old_locus_tag="pgaptmp_001546"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGU4703740.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDQLLTSLMSSQRQDK"
     gene            complement(35410..36840)
                     /gene="yihF"
                     /locus_tag="ACHKNP_RS07735"
                     /old_locus_tag="pgaptmp_001547"
     CDS             complement(35410..36840)
                     /gene="yihF"
                     /locus_tag="ACHKNP_RS07735"
                     /old_locus_tag="pgaptmp_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU4703741.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(36995..37621)
                     /gene="dsbA"
                     /locus_tag="ACHKNP_RS07740"
                     /old_locus_tag="pgaptmp_001548"
     CDS             complement(36995..37621)
                     /gene="dsbA"
                     /locus_tag="ACHKNP_RS07740"
                     /old_locus_tag="pgaptmp_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HGU4703742.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(37638..38624)
                     /gene="srkA"
                     /locus_tag="ACHKNP_RS07745"
                     /old_locus_tag="pgaptmp_001549"
     CDS             complement(37638..38624)
                     /gene="srkA"
                     /locus_tag="ACHKNP_RS07745"
                     /old_locus_tag="pgaptmp_001549"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HGU4703743.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(38701..38970)
                     /gene="yihD"
                     /locus_tag="ACHKNP_RS07750"
                     /old_locus_tag="pgaptmp_001550"
     CDS             complement(38701..38970)
                     /gene="yihD"
                     /locus_tag="ACHKNP_RS07750"
                     /old_locus_tag="pgaptmp_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HGU4703744.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            39040..39624
                     /gene="mobA"
                     /locus_tag="ACHKNP_RS07755"
                     /old_locus_tag="pgaptmp_001551"
     CDS             39040..39624
                     /gene="mobA"
                     /locus_tag="ACHKNP_RS07755"
                     /old_locus_tag="pgaptmp_001551"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HGU4703745.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            39606..40133
                     /gene="mobB"
                     /locus_tag="ACHKNP_RS07760"
                     /old_locus_tag="pgaptmp_001552"
     CDS             39606..40133
                     /gene="mobB"
                     /locus_tag="ACHKNP_RS07760"
                     /old_locus_tag="pgaptmp_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HGU4703746.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         9908 a        10213 c         9748 g        10323 t
ORIGIN      
        1 ctgatcatca acaccaacta cctccccctt ttgcctgcct ccctttgctt tacgcaccgg
       61 aaattgctgc cgctccagat attgctcaac cgcttcgcgt aaaattgcag atcgtgaaac
      121 cttacgccag ccccgaagtt tgttcagccg tttaattgcc tcttctgata aatgaagaaa
      181 aaccgtattc atcgccatta ccaccacctt acctatatta ctcattaatg tatgtgcgaa
      241 aattacgccg gaaaatcatt taaaaaaagc ggatttttgc atgttgtttt gtttttgcga
      301 ggcgttttcc agaaataata agagtgttgc agttgtttca tttattactc aaaaatggtt
      361 tagttatcgc ccaggatgag ttatggatgc tatgaatata ttatattcag ggatgtttat
      421 tttttgattg gatctttatt tgcataatct gaatggtgta cccgaaaaat gtcagcatcc
      481 taacagcacc cggatttaag gtgaaattaa tcatccatca cagaccggtc agtactgcca
      541 ccaaccggtc tttacttgtt catcgttata attttgttgg taacagttcg ggaaacaagg
      601 aattccttcc ggaacccccc ggtatcatcc gatagcattg acgtcaatca tcagcgcgac
      661 gatgagtttt tttaatcgca tcatagggtg gcgacgttca gcacgattac gggtcatagc
      721 aaaactcctg ggcaggcgac cactatgccg ctttcacagg agttctgaat gctagtgctt
      781 tctgtatggc ataaccatgc ctctaacggt taagtgcttg cagcacatgt tgcggatcat
      841 tggcaatcgc tcgcagcaaa gcctttgcag gcccggtcgg tgaacgtcgc ccctgctccc
      901 agttacgcaa cgtgtctacg cttacagaaa taagatcggc aaatttagat tgtgacaacc
      961 ctgaagcgcg gcgtatttct ttgattttca tagcatcaac ttcaaaagtc cgcgaaggct
     1021 gtcgcgttcc ttcaataatt tcattcatct gggccatgct gtgagtcaac cgctcaaaaa
     1081 gtgccttatc catttaccac ctcgtattta acaaccggag cattgctttt tcctggggag
     1141 acagatcatc tttaataccc ttacgataaa taagcagtaa tctgatctca taatgtttaa
     1201 cctgatgaaa gtaaataacc ctgacaccag cacgtttccc ctttcctcct gacacccatc
     1261 gaacctttct taacccacca gtttcaggta ttacctcacc atagtcagga ttcaaggcca
     1321 ggaaaaactg aaaacgactg aattcatcgt cggtcagcag tttttgaaca tcttcagtaa
     1381 aaatttccgt ttcgatgaag agcataatta tacgccattg gcttagagta acaatccttt
     1441 ttgtacgtca ttgacgtacc cttgaaacca ctagcttact gatagtgctc cagcaaaacc
     1501 acgtctctgc cgggtttgtc tttagatttg cgaggcgctt tccagatttt tcacgcccgt
     1561 gaatttccct gtatccatcc agcaaaaacg ggtgtataag actgtacatt catacagcaa
     1621 aataagttct actatggcac tggaaaaagg aattgcagag ttagtcgaaa agtttatcgc
     1681 tgcgggtcgc ccctcatcgc gtcaacaaag tattgcgcag cgcagggagg gctatatcgc
     1741 cagcgctgtc cttgcgggtg agacagaaac gcgggtagat attcagacta tcgaacttga
     1801 agggatgacc ttacgaatag tgtcgccgct gaatgccccg acattactcc cgacgataat
     1861 ttattactac ggtggctgtt ttgttagcgg cgggttcgca actcacgaca accagctgcg
     1921 ccagttagcc tattacgggc aatgtcgggt tattgccgtc cagtatcggc tggcaccgga
     1981 acatactttc cccgctgccc atgatgatgc gcaacgaggt gcagaaatag tctggcaaca
     2041 cgccgaacga ctcggcgttg ataaacaacg tataactctt gccggtgaca gtgcgggcgg
     2101 acatctggcg ctggtaactg ctttgcgatt aaagcgtgct ggcgagtggc aaccggcaca
     2161 actcatctta atttatccta tgcttgatgc gactgcgcat ttcgaaagct acatacgcaa
     2221 tggtcacgat tacatcatca cacgcgacac tttactgagc ggctttgaga tgtatctgcc
     2281 aggcattgaa cgcaggcatc ctgaagccag ccctctttgg cgcgatgatt tcaatggctt
     2341 gccacctgta catatcatta ccgctgaata cgacccactt tgcgatgaag gtgaagcgtt
     2401 gtatcaccgc atgacagggc aaggagtgca atgcacctgc caacgctacc ttggcgttat
     2461 ccacggattt tttcagttag gtggtatcag tgaggccgcg cgatcggcac tacgggatgt
     2521 tgcctggaga gcgggaaggt aacttcggac ctgggccctg aaaaggtttt cagattgaaa
     2581 accttttcag tcaagtaaaa cgcccctact cttcttcgtt cccgccctct tcatacgggc
     2641 caaatggctt cgcgggcaga acgatatacg tgccttcaaa cactgcaccc ggcgtctcgt
     2701 cgccaaagat ttcaacttgc atctgcaccc gtgcttttcg tccgcgcgcc agacggtcga
     2761 gatcgccgct taaggcacca aggtcggcta ccgcatgagg tttaccgcta atcggcttgc
     2821 tgtagcggat atgcgcatcc gccagaataa tcgttccgcc gagatggcgt tcgcgcagca
     2881 tcagccagat aagcccccaa ccggtgagcg ttgccagtga gaataaactc ccggcaaaca
     2941 gcgtatggtg cggattctga ttaccggttt ccggcatggt ggtgataaat ttttgcccgg
     3001 tatattgttg aatacgcacg cccatttttt cactaagcgg gatatgttcg taccacgcct
     3061 gttgcagctg cgcgcaccag tcgccgcgat gcaaaatatc atccagagtg gcgacgggct
     3121 taatcatcaa aaaatggcga atcggcgtgg tggttggcgt ggtgatttct ccctgattaa
     3181 caaaccccag cttggcgaaa aactccaccg cgtcttcacg ggcgctacag gtcacgcgct
     3241 taacgccttc ctgacgcgcc accgactcca gggtcatcgc catcagcgtt cctaaccctt
     3301 tgtcctgcac gtccgggtga acggccataa agcgaatgga cgcttcattg tcggcattaa
     3361 tatacaatcg gcctaccgcc accagattac cctgctcgtc gacgaccatc tggtgatgcg
     3421 ccatcgcatc ccacgcgtcg cgttccgaac cttttggttg atgcaggggc ttacgcaaca
     3481 tttcccagcg aaactgatag taacgctcta actcttcttc tgtttgtgga acccgaaggt
     3541 gatacatagc gatactctct cttgtttccc gtgacaaccc tggaagctgg ctcatacctg
     3601 caaccagaat gtcacggggc catcattgac cagcgatacc tgcatatcgg cagcgaagcg
     3661 tcctgtttgc gtgttcatct cttgctggcg gcagcgttcg acgaaataat catataacgc
     3721 ctctgcgcga tccggtgatg cgcctttaga gaaacttggg cgcatccccc gttcggtatc
     3781 tgcggcgagg gtaaactggg aaaccaccag tacactgccg cccgcctgtt gcacgttgag
     3841 attcatcttg ccttcggcat cgctaaagat gcggtagccg agcacacgct cgcacagacg
     3901 gtttgctttc tgttcgtcgt catccttttc gacacccaat aacaccaaaa gtcccgcgcc
     3961 aatttcgccc gtcacttctc cctccacggt gacgctggca cgggttacgc gttgaattaa
     4021 tgcaatcatg gttcgtcgtc ttcttcttgt tcagctgctt gttttagttt gcggtattcc
     4081 ccgagagtga cagtaatttc cgcgccaagc aagacgatac accatgtcca gtagacccaa
     4141 acaaagagaa tggggatcac cgccaacacg ccgtaaatga gctgatatga cgggaacatg
     4201 gtgatataaa gcgcgaaacc tttctttcct gcttcgaaca ggagcgcggc gacaaacgcg
     4261 ccgacaatcg cgtcgcggtt aggtacgcgg atggtaggaa caatgctgta cagcaaccag
     4321 aaggagatcc acgacaacag cagcggaaaa atacgcagca cgttatcgat gacagtattg
     4381 agatcgctcg cccagcgcag ggagagcaaa taggaactga tcgccagact ggcccctgcc
     4441 agcagcggcc ccagcgttaa aatcatccag tacacggcga acgagtaaat ttttggtcgc
     4501 gctcgtttac tgcgccagat ggtattcaac gcgctatcga tggagtacat cagcaataac
     4561 gcggtgacga tcagcccgca cgccccaacg gcggtcatct tgttggaatt ggcaacaaat
     4621 tgttcgatat accgctgaat aacatcgcca gtagcaggca gaaagttggc aaaaataaag
     4681 tgacgtaact gaatgctgac gtcggaaaac atgggaaaag cggcgaaaag cgcaaaaaca
     4741 acggcaacca gcggcactaa tgagagcaac gacacatagg caaggttacc tgccagggtt
     4801 gtcatgttgt cctcatcaat acgttgccag agtagtttta gccaggccca tagtggacgg
     4861 gtacggtgcc tggctttgtc ctgaatggtt tttagcataa caccttcgcg aaatagtccg
     4921 ggatggtggt tttatctttc accagaatac tggtaatgcc cagctgattg gctccttcta
     4981 tattatcggc gttatcgtcg aaaaagaccg tatcgctggg tgaaaaacct tccgcctgca
     5041 aaacatgctg gtaaattcgt gcttcaggtt tgcgcatccc cagatcttgc gacagataga
     5101 tatggtcagc agcatcacga atttccgggt attcttccgg ccagaaggtg gtatgcaggc
     5161 ggttagtatt ggaaagcacc accacgcgat gcccctgctc acgcagttta tgcatgatgg
     5221 cgatcacttc cgggcgcagc gcaacaaaca ccgcctgcca gccgtgagag aactgctcgt
     5281 agcttagcgg cagggccatc tcatgacaca gcgcctctgc gaacgcttcg tcgctaattt
     5341 ccccacgctc atgctgatga aacgcctccc ccatgtggaa actcttctta agcgtagcca
     5401 gtggaacacg cgttaaatcg ctccaggctc ccagcacacg gttaaagtcg atatcgacaa
     5461 tcacattacc taaatcaaag atatagagca tttttgcctc ctttcgcgcc acaagaaatt
     5521 aactgtagcg ggaaaggatg ggtttggcta tgtggcaagg tatgaagcca ggttaacggt
     5581 cacaaaaatc tacgcgtctt cctggggaag aatgatttca atttggctgt cttgtaagcg
     5641 tgattttagc gcagcgggcg gcagctcgtc ggtaaagagc gcagtgacct gtgccacgtt
     5701 accaatttca accgccgccg aagcatgata cttagtgtga tcggcgacca gcagaatatt
     5761 tctcgcgtgc gccatcatcg ttttcaccac gttagcttcg tttacatcaa actccatcaa
     5821 cgcgccatcg ctctcaatcg ccccaacgct tgttaccaga taatcagcac gaaaatcagc
     5881 cacaaaggac gccgctgaag ggccaatgat cccgctatta tgagagcgca acgtaccgcc
     5941 gggcaccatc acttcaaagc gcgggttgtg gtaaagaata tgcgccacac gcaggctgtt
     6001 ggtgattatc cgcaaatgat tatggttaag taacgcccgg gcaacatgct caacagtcgt
     6061 accaatggtg ataaatattg ttgaaccatc aggaatatag tctgccaccg cttcggcaat
     6121 cgcttttttt tcctcggttt gcgaaacctc acgctgctcg aacgccgtat taacgacgct
     6181 ggaagcccga cccgcgccac cgtgatggcg cgtaatcagg ccttgctcgc ttaatttacg
     6241 aatatcccgg cggaccgtct gcgtggagac atccagcaga tttgccagct catcaatatt
     6301 catataccca cgctcggcga tcagcatgag gagttggtcg tgccgcgggt taccggttag
     6361 ttcggtaagg ctcatgaaaa ttcctcgaaa aaccatcacc ctggcatttt atacaaaaag
     6421 tgacaaaaaa gatcgggttt gatcacagtc agggatcccg gctcgtccac cgggacgtgt
     6481 gcattttaac gccgctacac cgctggcgaa gcggactgac tccgctaaat ccccacttgt
     6541 tgccagcgcc accgccaaag cgccgtgaaa aacatcaccc gcacctgtgg tatctaccac
     6601 atcaactttg aaggccggtt gatgctgacg cccaccattt tccagccagt cgcagcctgc
     6661 gctaccctgg gtcacataga catgaccatt tgtgagcgtt tgtgcctgtt ttagcgcact
     6721 ggccatctct ttcacgcccg ttaagcgcgc cagacccggt tctgaaaagg ccgcgtgatc
     6781 gcttaatgcc accagctcac tgatatcctg cggcgtaatg tccccgtcca gaacggtcat
     6841 cacacccgcc tgacgggcca gggtgaaggc ttttttagcg ccgtcgtgcc agcgtacatc
     6901 tgccagcaca acatcccact gagagaaatc aatttcctcc aaccactctg cgtcaggcag
     6961 cagatccggg ctggggtagt taatgattat ccgctcgcct ttggtatcca ccatgatggc
     7021 ggattgcgaa gatttcgcct ggttataccg tttggtgtaa cgggtgttaa cgccccagga
     7081 ttccagctct gccagcaggc tgttgcccgt gtcgtcatca cctacgcgac caataaaatc
     7141 gacctgcgcc cccagccttg ccgccgcaac cgctgccgtc gccgctggcc cgccaccaac
     7201 ttccgtataa tttctcgcca cgtatttacc gctctccgtc ggtaaccctt ccacgtaata
     7261 gatgcgatcc atcacggtta tacctacaca agcaacacga atcatggtca ttccttaaac
     7321 attttgggat gcgttcattt taatttcacc aattgtttaa aaagtgacgg ctgtcaattt
     7381 tttgacgata aattacaaat acgatcaaaa acagacaagc aaagcatgtc ataaatgaca
     7441 aaaagtgaca tcactgtatt caggagaggg ttatggcagc aatcgcgttt atcggtttag
     7501 gacaaatggg ttcgccaatg gcgagcaatt tattgcagca agggcatcaa cttcgcgtct
     7561 ttgatgtgaa cgccgaagct gtgcggcatc tggtagacaa aggtgcgact cccgccgcca
     7621 acccggcgca ggccgctaaa gatgccgaat ttatcattac catgttgccg aatggcgatc
     7681 tggtgcgcaa cgtgttgttc ggtgaaaacg gcgtttgcga aggcttatct accgatgcgc
     7741 tggtcattga tatgtccacc atccatccgc tgcaaaccga taaattgatt gccgatatgc
     7801 aagccaaagg cttcagcatg atggatgttc cggtaggccg tacttctgca aatgccatta
     7861 ccggtactct gttactgctg gctggcggca ccgctgaaca agttgaacgt gccacaccga
     7921 tcctgatggc gatgggcagt gagttgatca acgctggcgg tccgggcatg gggatccgcg
     7981 ttaagctcat caacaactac atgagcatcg cgctcaatgc gctttcggca gaagccgccg
     8041 ttttgtgcga agccctgaat cttcccttcg atgttgccgt caaagtgatg agcggtaccg
     8101 ccgccggtaa aggtcacttc accacttcct ggccaaacaa agtcctcagc ggagatcttt
     8161 ctcccgcctt catgatcgat cttgcccata aggatcttgg catcgccctt gatgtcgcca
     8221 accagctgca tgtgccaatg ccgctggggg ccgcctcacg ggaggtttat agccaggcgc
     8281 gcgcagcggg tcgcggtcgc caggactggt ccgccattct ggaacaggtc cgtgtcagtg
     8341 ccgggatgac tgccaaagta aaaatgtaac gactggataa aggaataaat gaatgaataa
     8401 gtacaccatc aacgacatta cgcgcgcatc gggcggtttt gccatgctgg cggtcgatca
     8461 gcgcgaagcc atgcgcatga tgtttgccgc ggctggggca cccgctccgg tagccgatag
     8521 cgttttaact gatttcaaag ttaacgctgc aaaggccctc tcgccttatg cctcggcgat
     8581 tctggtagat caacaattct gctaccgcca ggtggttgag caaaacgcga ttgccaaaag
     8641 ttgcgccatg attgtcgccg ccgatgagtt cattcctggc aacggtattc cggtcgatag
     8701 cgtggttatt gaccgcaaaa tcaatccgct acagatcaaa caagacggtg gcaaagcctt
     8761 aaaactgctg gtgctgtggc gtagtgatga agatgcgcag caacgtctgg atatggtgaa
     8821 agagttcaac gaactgtgcc actcacacgg tctggtaagc atcattgagc cagtcgtccg
     8881 cccaccgcgt cgtggcgata aattcgatcg cgaacaagcg atcatcgatg ccgccaaaga
     8941 gctgggcgac agtggcgctg acctctacaa agttgaaatg ccgctttatg gcaaaggtcc
     9001 gcaacaagag cttctctgtg cttcacaacg tctgaatgac catatcaata tgccatgggt
     9061 gatcctctct tccggtgtcg acgaaaaact gttcccgcgt gccgtacgcg tggcaatgac
     9121 ggcgggcgca tcgggattcc tggcaggccg tgcagtctgg gcatcggtcg tcggtttacc
     9181 agacaacgag ctgatgctgc gtgacgtttg cgcaccgaaa ttacaacaac ttggcgatat
     9241 cgtcgacgaa atgatggcta aacgccgcta agatgaggat aaaaggaatg aaatggttta
     9301 acaccctaag ccacaaccgt tggctggaac aggaaaccga ccgcatcttt gattttggta
     9361 aaaattcagt agtgccgact ggttttggct ggttaggcaa taaagggcaa atcaaagaag
     9421 agatgggcac ccatctgtgg atcaccgctc gtatgttaca cgtttattcc gttgctgcgg
     9481 cgatgggtcg acctggcgct tactcgttgg ttgatcacgg tatcaaagcc atgaacggcg
     9541 cactgcgtga taaaaaatat ggcggctggt atgcctgcgt aaatgacgag ggcgtggtgg
     9601 atgcctccaa acagggctat caacacttct ttgctctgct gggtgccgcc agcgccgtca
     9661 caacgggtca cccggaagcg cgcaagctgc tcgattacac cattgaaatt atcgagaaat
     9721 atttctggag cgaagaagag cagatgtgcc tggaatcctg ggacgaagcc ttcagcaaaa
     9781 ccgaagagta ccgcggcggc aatgccaata tgcacgcggt ggaagctttc ttgattgttt
     9841 atgacgtcac tcacgacaaa aaatggctgg atcgcgcgat tcgcgtggct tccgtgatta
     9901 tccacgacgt cgccagaaat aatcattatc gcgttaacga acacttcgat acccagtgga
     9961 atccgctgcc ggattacaac aaagataacc cggcgcatcg cttccgcgcg ttcggaggta
    10021 caccaggcca ctggatcgaa tggggccgtt taatgctgca catccatgct gccctggaag
    10081 cccgttgcga acaaccacca gcatggctgc tagaagatgc caaaggtctg tttaacgcca
    10141 ccgtgcgcga tgcctgggca cccgatggcg cggacgggat tgtttatacc gttgactggg
    10201 aaggaaaacc ggtggtccgc gaacgtgtac gttggcctat cgtcgaagca atgggtacgg
    10261 cctacgcgct ctacaccgtc actggcgatc gccagtatga aacctggtat caaacatggt
    10321 gggagtactg cattaagtac ctgatggact atgaaaatgg ttcctggtgg caggagctgg
    10381 atgcggacaa taaggtcacc accaaagtct gggacggcaa acaggatatt tatcacctgc
    10441 tgcattgcct ggtgatcccg cgtatcccgt tagcgcccgg catggctcca gcggttgcag
    10501 cgggtctgct ggatattaat gcgaaataac tgatctgaaa atagggtgcg ggttttcccc
    10561 tcaccctaac cctctcccca aaggggcgag gggaccgtcc ggtgcagttt ttgggtttga
    10621 catcagcctc acgggaggct gtatgtcgtt aattaaggtg ccttgtatgc aaatcaccaa
    10681 tatgcattgc agtggacaga ccgtttcact tgccgcgggg gattatcacg cgaccattgt
    10741 gaccgtcggt gctggtctgg ctgaattaac ctttcagggt tgtcatttag tcatcccgca
    10801 caaacccgaa gagatgccgc tggcgcatct gggtaaagtg cttattccgt ggccgaaccg
    10861 gatcgccaac ggctgttacc gctatcaggg gcaggaatat cagttaccca ttaatgaaca
    10921 tagttcaaaa gccgctattc acgggctgct tgcctggcga gactggcaaa ttagtgaatt
    10981 aaccgccacc agcgttaccc tgacagcttt tctaccgccc agttacggct atccgtttat
    11041 gctggcttca caagttgttt attcgctcaa cgcacacacg ggtctttccg ttgagatcgc
    11101 aagccagaat atcggcactg tcgcagcgcc ttatggcgtg ggtattcata cttatttaac
    11161 ctgcaactta acatctgttg atgaatatct gttccagctt ccggcaaacc aggtttatgc
    11221 cgttgatgaa cacgtaaatc cgacaacgct tcaccacgtt gatgagctgg atttaaattt
    11281 cacgcaggcg aaaacaatag ccgcgacaaa aatcgaccac acctttaaaa ccgctaatga
    11341 tctgtgggaa ataaccatta cccatccaca acaggcgctt tcggttagcc tttgttgcga
    11401 tcaaccctgg gtacaaattt atagcggtga aaaattacag cgtcagggac tggcagttga
    11461 acctatgagt tgcccgccta acgcctttaa ttcaggtatc gacttattat tactggaacc
    11521 aggaaaaccg caccgcctgt tttttaacat ttacggacag agaaaataac ttgtattgat
    11581 tagcagtcaa aatcattgaa gaacatttca ctcgctaaag gcaacattat gtatcttcaa
    11641 tttattaagt taaaaacagc tcagcttctg taaagttttt atatatcagt tttatgagaa
    11701 tcattttaca taagcctgta ataacaggaa ggcaacaatc tcgttctcaa ctctatggag
    11761 attagttatg gatacgccac gtccacagtt attagatttt caatttcatc agaataacga
    11821 cagttttacc ctacattttc aacaacgtct tattttaacc catagcaaag ataatccttg
    11881 tttatggatt ggctcaggta tagcggatat cgatatgttc cgcggtaatt tcagcattaa
    11941 agataaacta caggagaaaa ttgcgcttac cgacgccatc gtcagccagt caccggatgg
    12001 ttggttaatt catttcagcc gtggttctga cattagcgcc acgctgaata tctctgccga
    12061 cgatcagggc cgtttattgc tggaactaca aaacgacaac cttaaccaca accgtatctg
    12121 gctgcgcctt gccgctcaac cagaggacca tatctacggc tgcggcgaac agttttccta
    12181 cttcgatctg cgtggcaaac cgttcccgct atggaccagt gaacaaggcg ttggtcgcaa
    12241 caaacaaacc tatgtcacct ggcaggccga ctgcaaagaa aatgcgggcg gcgactatta
    12301 ctggactttc ttcccacagc ctacgtttgt cagcacgcag aagtattact gccatgttga
    12361 taacagttgc tatatgaact tcgactttag tgccccggaa taccatgaac tggcgctgtg
    12421 ggaagacaaa gcaacgctgc gttttgaatg tgctgacaca tacatttccc tgctggaaaa
    12481 attaaccgcc ctgctgggac gccagccaga actgcccgac tggatttatg acggagtaac
    12541 gctcggcatt cagggcggga cggaagtgtg ccagaagaaa ctggacacca tgcgtaacgc
    12601 gggcgtgaag gtcaacggca tctgggcgca ggactggtcc ggtattcgta tgacctcttt
    12661 tggcaaacgc gtgatgtgga actggaagtg gaacagcgaa aactacccgc aactggattc
    12721 acgcattaag cagtggaatc aggagggcgt gcagttcctg gcctatatca acccgtatgt
    12781 tgccagcgat aatgatctct gcgaagaagc ggcacaacac ggctatctgg caaaagatgc
    12841 ctctggcggt gactatctgg tggagtttgg cgagttttac ggcggcgttg tcgatctcac
    12901 taatccagaa gcctacgcct ggttcaagga agtgatcaaa aagaacatga ttgaactcgg
    12961 ctgcggcggc tggatggctg acttcggcga gtatctgccc accgacactt acctgcataa
    13021 cggcatcagc gccgaaatta tgcataacgc ctggcccgcg ctgtgggcga aatgtaacta
    13081 cgaagccctt gaagaaacgg gcaagctcgg cgagatcctt ttctttatgc gcgccggttc
    13141 taccggtagc cagaaatact ccaccatgat gtgggcgggc gaccagaacg tcgactggag
    13201 tctcgacgat ggcctggcgt cggttgtccc ggcggcgctg tcgctggcaa tgaccggaca
    13261 tggcctgcac cacagcgaca ttggcggtta caccaccctg tttgagatga agcgcagcaa
    13321 agagctgctg ctgcgctggt gcgatttcag cgccttcacg ccgatgatgc gcacccacga
    13381 aggtaaccgt cctggcgaca actggcagtt tgacggcgac gcagaaacta tcgcccattt
    13441 tgcccgtatg acctccgtct tcaccaccct gaaaccttac ctgaaagagg ctgtcgcgct
    13501 gaatgcgaag tccggcctgc cggttatgcg cccgctgttc ctgcattacg aagacgatgc
    13561 gcacacttac accctgaaat atcagtacct gttaggtcgc gacattctgg tcgctccggt
    13621 gcatgaagaa ggccgtagcg actggacgct ctatctgccg gaggataact gggtccacgc
    13681 ctggacgggt gaagcgttcc ggggcgggga agttaccgtt aatgcgccca tcggcaagcc
    13741 gccggtcttt tatcgcgccg atagcgaatg ggcggcactg ttcgcgtcgt taaaaagcat
    13801 ctaatcacat ccgcccgtgg ggaaactcat gggcaaaggg agaacatcga tgagtcacat
    13861 cacaacggaa gatccagcaa ctttacgcct gccctttaaa gagaaactct cttacggtat
    13921 tggcgacctg gcctctaaca tcctgctgga tatcggtacg ctttatcttt tgaagtttta
    13981 taccgacgtt ctggggctgc caggcaccta tggcggcatt atctttttga tttcaaaatt
    14041 ctttactgcg tttaccgata tgggtaccgg cattatgttg gattcccgac gcaagatcgg
    14101 tccaaaaggt aagttccgtc cttttattct gtatgcgtca ttcccggtca ccttactggc
    14161 gatcgccaac tttgtcggca caccgtttga tgtcaccggt aaaacggtga tggccactat
    14221 tctgtttatg ctttacggac tgtttttcag catgatgaac tgctcctacg gcgcaatggt
    14281 tcccgctatc accaaaaacc ccaacgaacg cgcctcactg gcggcatggc gtcagggcgg
    14341 cgctacgctg ggcctgctgc tgtgcacggt gggattcgtg ccagtgatga atcttatcga
    14401 gggtaatcag caacttggct atatcttcgc cgccacgctg ttttcactgt tcggcctgct
    14461 gtttatgtgg atctgctact caggcgtgaa agagcgttat gtcgaaaccc aaccaaccaa
    14521 tccggcgcaa aagcctggcc tgttgcaatc tttccgcgca attgccggta accgcccact
    14581 gttcattctg tgtattgcca acctctgcac cttaggggcg tttaacgtca agctcgccat
    14641 tcaggtctat tacacccagt acgtacttaa cgatcccatc ctgttgtcgt atatgggatt
    14701 tttcagcatg ggctgtattt tcatcggcgt gttcctgatg cctggcgcag tcagacgttt
    14761 tggtaagaag aaggtctata tcggcggcct gctgatttgg gtgctgggcg atctgctcaa
    14821 ctatttcttc ggcggcggct cggtcagctt tgtggcgttc tcctgcctgg cgttcttcgg
    14881 ctcagcgttt gttaacagcc tgaactgggc gctggtttcc gacaccgtcg agtacggcga
    14941 gtggcgcacc ggcgtgcgtt cggaaggcac ggtctacacc ggcttcacct tctttcgcaa
    15001 agtgtctcag gcgctggctg gtttcttccc cggctggatg ctgacgcaaa ttggctatgt
    15061 gccgaacgtc gcacaggctg accacaccat tgaagggtta cgccagttga tcttcatcta
    15121 cccaagcgca ctggcggtag tcaccattgt ggcgatgggt tgcttctaca gcctgaacga
    15181 gaagatgtat gtccgcattg tggaagagat agaagcccgt aaacgcacgg cgtaataata
    15241 ataaacgacg ccctgcgggg cgttataagg agtgattatg tctgaccata atccactgac
    15301 attaaaactg aatctgcggg aaaaaatcgc ctatggtatg ggcgacgtcg gttcgaattt
    15361 aatgctctgc atcggtactc tgtatctcct caaattttat accgatgaat tagggatgcc
    15421 tgcttactat ggcgggatta tctttctggt ggcgaagttt tttaccgcgt ttaccgatat
    15481 gctcaccgga tttttactcg actcgcggaa aaatattggg cctaaaggca aatttcgtcc
    15541 ctttatttta tatgcagcag ttccggcggc gttaattgca acgctccagt ttatcgccac
    15601 caccttttgt ttgccggtta aaacgacgat tgccaccgcg ctgtttatga tgtttggact
    15661 ttcatacagt ctgatgaact gctcgtatgg tgcgatgatc ccggcaatta ccaaaaaccc
    15721 gaatgagcgc gcgcagctcg cggcttaccg ccagggtggt gcaaccatag ggctattgat
    15781 ttgtaccgtg gcgtttattc cgttgcagtc gcttttttct gactcaaccg tcggttatgc
    15841 ctgtgcggca cttatgttct ccattggcgg ctttattttt atgatgctgt gctacagagg
    15901 cgtcaaagag cattatgtgg acacagcgcc aaccggacat aaagccagta ttctcaaatc
    15961 tttttgcgcg atatttcgta atccaccatt gctggtttta tgcattgcta acctgtgtac
    16021 tctggcggca tttaatatca aactggcgat tcaggtctat tacacccagt atgtgctgaa
    16081 tgatattaat ttattgtcgt ggatgggatt cttcagtatg ggatgcatcc tcgtcggcgt
    16141 attactggtg ccagtaactg taaaatgttt tggtaaaaaa caggtttatt tagctggcat
    16201 ggtgctatgg gcggtgggtg atatactgaa ttatttctgg ggaagtaact ctttcacttt
    16261 cgtcatgttc tcttgtgtcg ccttttttgg cacggccttt gtaaacagcc tgaactgggc
    16321 actggtacca gataccgtag attacggtga atggaaaacc ggtattcgtg ccgaaggttc
    16381 tgtttatacc ggttatacct tctttcgtaa aatttctgcc gcacttgctg gcttcttgcc
    16441 aggcattatg ctgacgcaaa ttggttatgt tcccaacatc gcgcaaagcg atgcgacatt
    16501 acagggtttg cgtcagctca tttttatctg gccttgtgca ctggcaatta ttgctgcatt
    16561 gacgatggga ttcttttaca cactcaatga aaaacggttt gcattgatta ttgaggaaat
    16621 caaccaacgc aaaaataaag agatggcgac agaagaaaaa acggcttccg taacgttata
    16681 aatgtcaata gccgctattt ccatcctggt gggtggcggc ctccctacgt ttaaaaaatg
    16741 gacttattat gaaaaagatt aatgcaataa ttttattatc ttctctgacc tctgcctcgg
    16801 tatttgctgg cgcatacgtc gaaaacaggg aggcatataa tcttgcctcc gatcagggcg
    16861 aggttatgct acgtgtgggt tataacttcg atatgggcgc gggtattatg ttaaccaata
    16921 cctacacctt tcagcgagaa gatgaactaa aacatggcta taatgaaatt gaaggttggt
    16981 atcctttatt caaaccaacc gataaattaa ccatccagcc cggtggctta attaatgata
    17041 agagtatcgg ttcgggtggt gcagtgtatc tggacgtcaa ctataaattt gtaccatggt
    17101 ttaatctgac agtacgaaat cgctataacc ataataacta tagttcaaca gatttgagcg
    17161 gggaactgga taataatgac acctatgaaa ttggcaccta ctggaatttt aaaatcaccg
    17221 ataaattttc ctatacattt gagccacatt atttcatacg cgtaaatgac tttaatagca
    17281 gcaatggaaa agatcatcac tgggaaatca ctaatacctt ccgttatcgc attaatgaac
    17341 actggctgcc ttatttcgaa ttacgttggt tagatcgtaa tgtcgaaccg taccaccgcg
    17401 agcaaaacca gattcgtatc gggacgaagt atttcttctg atgtgaaact gccggatgcg
    17461 gcgtaaacgc cttatccggc ctacggttga tacggttttc gttggccgga ttaagcacat
    17521 ccggccatta atcaagcttc agccgccagc atctctttct tacgttggct aattcgctta
    17581 accagcatta cggaaaccac cgcaccacag aatgcgaagc aggccataat gccaaacacg
    17641 attttgtagc caataatccc cggatttaaa tccagaatgt agccatacag actgaagcag
    17701 aacatcgccg gagcgtaacc aataaagcta cccaacgcca tcgccgcgcc tgttttattt
    17761 tcagcaattt ttgcttcgcc gataggtgca aaaaatacgg ctcgctgtgt aaagactatc
    17821 gcgccaaagc ccagcgtaca tgccatccct aaatagaccg gcatactttc gtgcggcagc
    17881 ataatcaaca gtacgagcgc agcggtactg atgataaagg tgtagcatag atatttactc
    17941 ggcgatttca ggatcttatc tgaaatcatg ccaccaatcg gtccaccaat cattttcaga
    18001 cagtattggt taatgatgcc gtaagccccc accagcgcaa cgggcaatag atagatgttt
    18061 ttcaggaatg gaatgaagaa tgtcaggcca cagtaaaccg cgtaaacgaa gaagacgtta
    18121 aaagcgataa gccagatggt tttgtctctc agcaccgagg tcatactggt gttttgcgat
    18181 gctccgtctt cttttttcac ctcaacggac ggtgcctctt ctttgtcatt caggacaaag
    18241 aaaataataa tgcctacggc aatcacaata agggaataga accagatgcc tgctttaaaa
    18301 cctaataagc cactgccaaa ccaggtaaat actgccaacg cagaaaatgc caccacggta
    18361 tcgacaatgc cacgccctgt ttcgaagaag ccaaacaacc gaccttgttg ttcgctattg
    18421 cccaatcgac ttaccgactt gagcaagacc ggccagttca tcatgtcgca agtaacgcca
    18481 aatagcgccc agacaaagag gatgccccaa taccccggca ttgtcgtcaa ataaacaccc
    18541 agtaatcctg tcgcaatgag tgagaatgac atggtgtatc tgcgcggtag tttatcggca
    18601 aaataaatag acagaaaaaa gcccactgtg gtgacaaatg agtttaccga catagcatta
    18661 ccaatttgac cattggtcaa atggaaatat tcctgcatcg ggatataaaa cgcatctttc
    18721 agcgacggta atttataaat tgtaccgcca cacaatgtta atagactaaa taacgcccat
    18781 ttctttttcg tgagcataac atcccctaag cgatttttaa aatcactgtt ttaaaggcaa
    18841 taagataccg accattcata tggaatggtt ttattaactg cgtatgagtt aaaacttatt
    18901 agtggttcag aagatggttt gcctcttgtt tgatttgttg tagtacgtta cgcacatgat
    18961 ttatctgatt aattgcatct tctttttctt tacgtaaccg cgcatccagt aaatgatttt
    19021 ctggcaatgg tgtttcactc atctggcgat aagggatcca cggattatca tcttcgtctt
    19081 caaagcggaa cgccggcgca tagtaatcaa cctcttcttc caggccatat gccgtcacct
    19141 gcggctcatc atcacccagg catataaggt gcgttaataa cgctttgaac ggcatatcac
    19201 cctgacctga aatacaggct ttatgaccca ggccacccgg ttctttaacg atcaaggcat
    19261 ctttgatatg gacctgggta atatgcggcg ccatcgtttt taaagcgtcg atgggatgct
    19321 catttgcatt gatcatgttc gcaaaatcaa acagtaagga aagagattcc atctcacttt
    19381 ctttgaccag cgacaccagc tcatggcttt ttaaatcttc atgctgttcg atcgtaaaag
    19441 tcaggccgct gtcctgatac gtttcccgta cataggcaat gtcgttagcg atgatcgata
    19501 atacgtcgcg caaattacct tcataacgtg gataaaaacg tacggacgat gccccagttt
    19561 tcaacgcaat ggcgacggct tcgtcaatag atgccttatc tgaagcgctg gtttcaatat
    19621 gaatatcaag gttcagacgg cgggctttat ccccaaaggc agagagttct ttatcgtcca
    19681 tattaccaag agaaaaacgc tcgccatcca gaacatggat tttgacgcca cgtagattat
    19741 tttcacttgc gatatcaagt aaatcagcag gcaacactct ttccagccgc atatttaaat
    19801 gaaaggcata agcatgtaaa taaagaggaa gagtgtcgac acgttgtaga atctttcttg
    19861 cgttatttat tgtcaccata aaacacctac agattaatga tgaggtctac ggcctcaaat
    19921 cgccaatatt caatatctat ctggacaata ttgccttgct ggtcggcgcg atgcttttca
    19981 attaagatcg ctggcatacc cgcagaaccg ccaagatact tgctgatatc gcccggcatt
    20041 ctgacgggtt tgaaaatcaa tttttttact accgtctctt tttggtagca cttttcccag
    20101 actgcagaaa atgagtggtt ttcaagttgt tcaataaaac ccggagcaac ttcaggatta
    20161 atatatgttt cgtgatagaa aactttatgt ccttccagcg ccccccagcc ggtgatgcga
    20221 tagagttcag ttgatggtgt cactgcaatc agtggcgcga gcgtcttggg aatatcgctg
    20281 gtacggtttt tctcggtaaa cccccaggaa ggctctcgtc cttgctcaat tgcggcccgc
    20341 tgaaagctcg ccgacaactc cggactgtaa ttaaatcgcg gctgggtcac gaaccacccc
    20401 ttacgatcct tacggaagat tttggattct gcctcgaggt tcaacaacgc ctggcgcagc
    20461 gtcatacgtt taatgcccag cagttctccc agctctcttt ctgaagggag ttttccaccc
    20521 ggcgtggtga tgccatcttt caaccaccga tccagtttct cttttgcatt ttctacggtg
    20581 gattgattct ccgccattgt ttaatccttt tggtttaaac cagatgaagc atcttacgct
    20641 tacgccaaaa aagaaagcaa ttaacggcgt atgactttgc agataattgt gacaaagctc
    20701 tctattgacg taatcccaga cttataaggg agatactcgc attggtgtgc gctcacgaca
    20761 ggtaaaaaaa aacctgccag cgatggcagg ttttttataa ctaaaaaacg aaattaatcg
    20821 tctttcggtg cgcggttggc gcggcgacga tcgttttccg tcaggtgacg tttacgaata
    20881 cggatagagg tcggagtcac ttctaccagt tcgtcatcat cgatgaactc cagagcttgt
    20941 tccagagtca tgcggatagg cggaaccaga acaacggctt cgtcagtacc ggaagcacgc
    21001 atgttggtca gtttcttacc ggtcaggcag tttaccgtca ggtcgttaga gcggctatga
    21061 ataccgataa tctgaccttc gtaaacttct gcaccgtgac cgaggaacag cttaccgcga
    21121 tcctgcagac cgaacagcgc gaacgcgacc gctttaccct gaccgttaga gatcagtacg
    21181 ccgttctgac gctgacccac ttcacccgga cgtacgtcgt cgtagtggct gaaggtggag
    21241 tacagcagac cagtaccgga agtcatggtc atgaactcag aacggaagcc aatcagacca
    21301 cggcttggga tcacgtagtc gagacgtacg cggcctttac cgtctggatt catgtttttc
    21361 aggtcgcctt tacgttcgcc cagcgcctgc attacagaac cctgatgctg ttcttcaacg
    21421 tccagagtca cgttttcata cggctcttgt ttacgaccgt cgatttcacg gaagataact
    21481 ttcggacggg ataccgccag ttcgaaacct tcacgacgca tgttttcgat cagaacagac
    21541 aggtgcagtt cgccacgacc agaaacgcgg aacgcatcgg cgtcttcggt ttcttctacg
    21601 cgcagcgcaa cgttgtgtac cagttctttg ttcagacgat ccaggatctg acgagacgtt
    21661 acgaacttac cttctttacc gcagaacggc gaggtgttaa cgcagaagaa catagaaacg
    21721 gtcggctcat caacggagag tgccggcagc gcttcaacgt tttgcgtgtc gcaaacggtg
    21781 tcagaaatgt tcagttcgcc aaggcccgtg atcgcaacga tatcgccagc ttccgccaga
    21841 tcggtttcga tacgttccag accgaggtgg cccagcactt taccgacttt cgcgttgcgg
    21901 gttttgcctt cgctatcgat gatagtgacc tgctggttcg gcttcacttt accgcgcttg
    21961 atgcggccaa tgccgataac gccaacatag ctgttgtaat cgagctgaga aatctgcatc
    22021 tggaacggac cgtcaaggtc aacgtccggc gcaggaacgt ggtcaacaat cgcctggtac
    22081 agcggggtca tgtcttccgc catatcttcg tggtccagac ccgcgatacc gttcagcgca
    22141 gaagcgtaaa cgatcgggaa gtccagctgc tcgtcggtcg cgtcgaggtt aacgaacaga
    22201 tcgaatacct gatccacaac ccaatcagga cgcgcgccag ggcggtcaac tttgttgata
    22261 acaacaatcg gcttcaggcc gtaagcaaac gcttttttgg ttacgaagcg cgtttgcggc
    22321 atcgggccgt caaatgcgtc aaccaccagc agcactgagt ctaccatgga cattacacgt
    22381 tcaacttcac caccgaagtc ggcgtgcccc ggggtatcaa cgatgttgat acggtaatca
    22441 ttccatttga tagcggtgtt tttcgcgagg atggtaatcc cccgctcttt ctccaaatcg
    22501 ttggagtcca tcacgcgctc ttgggtttcg gcacgagagt cgaacgtacc ggattgttgg
    22561 agcagcttgt ctaccagggt ggttttacca tggtctacgt gcgcgatgat ggcgatatta
    22621 cgcaattttt cgatcacaac tttgcctcag gcattagaaa tagcgcgtta ttgtacacgg
    22681 attaatcgca ctacaaaaca ggatcacaaa catcctccgc aaacaagtat tgcagagtcc
    22741 ctttgtgatc gctttcacgg agcataaaaa gggttatcca aaggtcattg caccaacatg
    22801 gtgcttaatg tttccattga agcactatat tggtgcaaca ttcacatcgt ggtgcagccc
    22861 ttttgcacga tggtgcgcat gataacgcct tttaggggca atttaaaagt tggcacagat
    22921 ttcgctttat cttttttacg gcgacacggc caaaataatt gcagatttcg ttaccacgac
    22981 gaccatgacc aatccaggag agttaaagta tgtccgctga acacgtactg acgatgctga
    23041 acgagcacga agtgaagttt gttgatttgc gcttcaccga tactaaaggt aaagaacagc
    23101 acgtcactat ccctgctcat caggtgaatg ctgaattctt cgaagaaggc aaaatgtttg
    23161 acggctcctc gattggcggc tggaaaggca ttaacgagtc cgacatggtg ctgatgccag
    23221 acgcatccac cgcagtgatt gacccgttct tcgccgactc caccctgatt atccgttgcg
    23281 acatccttga acctggcacc ctgcaaggct atgaccgtga cccgcgctcc attgcgaagc
    23341 gcgccgaaga ttacctgcgt tccactggca ttgccgacac cgtactgttc gggccagaac
    23401 ctgaattctt cctgttcgat gacatccgtt tcggatcatc tatctccggt tcccacgttg
    23461 ctatcgacga tatcgaaggc gcatggaact cctccaccca atacgaaggt ggtaacaaag
    23521 gtcaccgtcc ggcagtgaaa ggcggttact tcccggttcc accggtagac tcggctcagg
    23581 atattcgttc tgaaatgtgt ctggtgatgg aacagatggg tctggtggtt gaagcccatc
    23641 accacgaagt agcgactgct ggtcagaacg aagtggctac ccgcttcaat accatgacca
    23701 aaaaagctga cgaaattcag atctacaaat atgttgtgca caacgtagcg caccgcttcg
    23761 gtaaaaccgc gacctttatg ccaaaaccga tgttcggtga taacggctcc ggtatgcact
    23821 gccacatgtc tctgtctaaa aacggcgtta acctgttcgc aggcgacaaa tacgcaggtc
    23881 tgtctgagca ggcgctgtac tacattggcg gcgtaatcaa acacgctaaa gcgattaacg
    23941 ccctggcaaa cccgaccacc aactcttata agcgtctggt cccgggctat gaagcaccgg
    24001 taatgctggc ttactctgcg cgtaaccgtt ctgcgtctat ccgtattccg gtggtttctt
    24061 ctccgaaagc acgtcgtatc gaagtacgtt tcccggatcc ggcagctaac ccgtacctgt
    24121 gctttgctgc cctgctgatg gccggtcttg atggtatcaa gaacaagatc catccgggcg
    24181 aagccatgga caaaaacctg tatgacctgc cgccagaaga agcgaaagag atcccacagg
    24241 ttgcaggctc tctggaagaa gcactgaacg aactggatct ggaccgcgag ttcctgaaag
    24301 ccggtggcgt gttcactgac gaagcaattg atgcgtacat cgctctgcgt cgcgaagaag
    24361 atgaccgcgt gcgtatgact ccgcatccgg tagagtttga gctgtactac agcgtctaat
    24421 agttgaagtt gtactacccg gcgcaacaac gccggaggtt caaaccaggc ccatctggcg
    24481 taattgttgc agccagtttg aacacggaca gcgcgcagaa cccggagcgt acactggtac
    24541 gtgaggagtt cgagcactgc ccaggttcaa aatggcaaat aaaatagcct gatgggactg
    24601 gtttttagtt gccgtggaaa tttcagccca tctctgcatg ggcttttttc tccgtcaatt
    24661 ctctgatgct tcgcgctttt tatccgtaaa aagctataat gcactaaaat ggtgcaacct
    24721 gttcaggaga ctgctttatg gcaacaggca cgcagcccga tgctgggcag atcctcaact
    24781 cgctgattaa cagtattttg ttaatcgatg acaacctggc gatccattac gccaaccctg
    24841 ccgcgcaaca actgctcgcc caaagctccc gcaaattgtt tggtacaccg ttaccggaac
    24901 tgttgagcta cttctcatta aatatcgagc tgatgcaaga aagtctggag gcggggcaag
    24961 gttttaccga taacgaagtg acgctggtca tcgacgggcg ctcgcatatc ctttctgtga
    25021 cggcccagcg tatgccggac ggcatgatcc tgctggagat ggctccgatg gataaccagc
    25081 gccgcttaag tcaggaacag ctacagcacg cccagcaggt tgctgcccgt gatttagtgc
    25141 gcggcctggc acatgagatt aaaaatccgc ttggcggttt acgtggcgcg gcgcagctgc
    25201 tcagcaaagc gttacctgac ccatcactac tcgaatatac caaagtgatt atcgaacagg
    25261 cggaccggct gcgaaatctg gtcgaccgtc tgttggggcc gcagctgccc ggtacgcgcg
    25321 ttaccgaaag tattcacaaa gtggctgaac gcgtggtaac gctggtgtcg atggaactgc
    25381 cggacaacgt gcggttgatt cgtgattacg atcccagcct gccggaactg gcgcacgacc
    25441 cggatcaaat tgaacaggtc ttgctgaata ttgtgcgcaa tgcgctacag gcgctggggc
    25501 cggaaggcgg tgaaatcatt ctgcgtaccc gcaccgcgtt tcaactgacc ttacacggcg
    25561 agcgctaccg gctggcggcg cggattgatg tggaagataa cgggccgggc attccgcctc
    25621 atttgcagga tacgctgttt tacccgatgg tcagcggccg cgaaggtggc accgggcttg
    25681 gcttatccat cgctcgtaat ttgattgatc agcattcagg caaaattgaa tttaccagtt
    25741 ggccagggca taccgagttc tcggtttacc tgcctatcag gaaataaagg tgacgtttat
    25801 gcaacgaggg atagtctggg tagtcgatga cgatagttcc atccgttggg tgcttgaacg
    25861 tgcgctcgct ggggcaggtt taacctgtac gacgtttgag aacggcgcag aagtgctgga
    25921 ggcgctggcg agcaaaacgc cggatgtgct gctttcagat atccgtatgc cgggaatgga
    25981 cgggctggcg ctgctcaagc agattaaaca gcgccatcca atgcttccgg tcatcattat
    26041 gaccgcacat tccgatctgg atgctgccgt cagcgcctat caacaagggg cgtttgatta
    26101 tctgcccaaa ccgtttgata tcgacgaagc agtggcgctg gttgagcgcg ctatcagtca
    26161 ttaccaggaa cagcagcagc cgcgtaatgt tcagcttaac ggcccaacga ccgatatcat
    26221 cggcgaagcg ccagccatgc aggacgtgtt ccgtattatc ggtcggcttt cgcgttcttc
    26281 tattagcgtg ctgattaacg gcgaatccgg caccggtaaa gaactggtcg ctcatgccct
    26341 gcatcgccac agtccgcgcg ccaaagcgcc gtttatcgcg ctgaatatgg cagctatccc
    26401 aaaagatttg atcgaatcag aactgtttgg ccacgagaaa ggcgcgttta ctggcgcgaa
    26461 taccattcgt caggggcgtt ttgaacaggc cgatggcggt acattattcc tcgacgaaat
    26521 tggtgatatg ccgctggatg tgcagacgcg tttgctgcgc gtgctggcag acggtcagtt
    26581 ttaccgcgtt ggcggctatg cgccggtgaa agtggatgtg cggattatcg ctgccactca
    26641 ccagaatctc gaacagcgag tgcaggaagg taagttccgt gaggatctgt tccaccgcct
    26701 gaacgttatc cgcgttcatc tgccgccgct gcgcgaacgt cgggaagata ttccccgtct
    26761 ggcgcgccat tttttacagg ttgccgcgcg cgaactgggc gtagaagcga agttactgca
    26821 tccggaaacc gaagctgctc tgacgcgtct ggcgtggcca ggcaacgtgc gccagctgga
    26881 aaacacctgc cgctggctaa cggtgatggc cgccgggcag gaagtgttga ttcaggattt
    26941 gcccggcgaa ctgtttgaat caacggttgc ggagagtact tcgcaaatgc aaccggacag
    27001 ctgggcgacg cttcttgcgc agtgggcaga cagagcgctg cgttccggtc atcaaaatct
    27061 gctttccgaa gcgcagccag agctggagcg gacgttactg acgaccgcgt tgcgacatac
    27121 gcaggggcat aaacaggaag cggcgcggct actcggctgg ggccgcaaca ccctgacgcg
    27181 taagttaaaa gagctgggga tggagtgatt cacagcttgt gtgtaaagat tgattattga
    27241 gcgcaaattg ctggtatttt acgctttact gttccgataa gttcagtatg atcttgcccg
    27301 gaaaacgggg agagtcatta tgctggaatc aataattaat ctggtatcga gtggcgcagt
    27361 tgacagccac acaccgcaaa ctgccgttgc tgccgtgctg tgtgccgcaa tgattgggct
    27421 gtttagttga gtttcagatt gctgatgaag tgctctttgt tcatgccgga tgcggcgtga
    27481 acgccttatc cggcctacaa aagcaatatt ttcaacaggt tttgtaggcc tgataagcgt
    27541 agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc tactttgtaa
    27601 acgaagcgcc attcactatt taaatcaccc gtgagaactg ctgcatccgc gctttctggc
    27661 gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct ttcgccgtca
    27721 cctgtattcc cttctcatcc acatccacca gcccatcttt tgctaacggg gcgagcagct
    27781 tgagatcttc cgcaaagtaa tcagcgaagt gcaaatccca cagtttctca ataggggcgt
    27841 aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata cagtcatcac
    27901 gcgttagcgc aataccacgc cacagcgcat tgccttgttc atccacttgc tgatagtact
    27961 gcttcaactc tttctggttc tgcgcgtagc agtcgccaat catgctgatg gcggaaacgc
    28021 ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaagtta cgatgcagca
    28081 cgccttcacg ctgggccacc gccagctcgt catccggacg ggcaaagtga tccataccga
    28141 taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata tcgagttttt
    28201 gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa atggtcggca
    28261 gatgcgcgta gttaaagaca ctcagacgat cggggttcag ctccgccaca cgtttcaggg
    28321 taaaggcgaa actctccggc gtctgtttcg gcaggccgta aatcaggtcg atgttggtgg
    28381 aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa gatgaactct tcatcctgct
    28441 cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccatg ctcaggcgat
    28501 taaagccttc ggcgcgtaaa tgatcgagta catccagttc gatttcccgc ggatcgactt
    28561 cgatcgaaat ctccgcatcg gcattgaact ggaagttttc gcgcagcagc ttcatcaggc
    28621 ggctgatttg cgctttattc agatacgtcg gcgttccgcc gccccagtgc aattggctga
    28681 cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc gcgtccagat
    28741 actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag aagtaacaaa
    28801 gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga tagcgcgcca
    28861 cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg gtcgggtacg
    28921 aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc cagtcgattt
    28981 gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc gtaccggcgg
    29041 atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg acaaccgctg
    29101 tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca gcagaacggg
    29161 aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt cttcttgctt
    29221 ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct gcatcaactc
    29281 atcaatacga tccagtttag catccaccca ggattgctct tcggcgctca gggtttcgcc
    29341 tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttccagta attccaactc
    29401 cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg tgactttttc
    29461 agtcacgccc aatggaatag gggttttact gccaatacgt ggatcttttg gtgcgttctg
    29521 gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg catgaccacg
    29581 acgttttttc tgacgcttgc gatcacgagc ttcctgatcc agctcctcgc gtgtttttcg
    29641 acgcgctttt gcatgacctt tgctgcgtga gtttgaagat gatggtttca taatggttcg
    29701 tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa gcaagcaaag
    29761 aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct taacgggtct
    29821 tacaatcctt gcaggccgcc aacacaccct gtttttcctt tagccggaac aatccgtgtt
    29881 gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat cctctgtctt
    29941 cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg tcctcgctct
    30001 atgggtgcta ctttacgcct ttgctttaaa caaacaatca accgccatca gtatttgcac
    30061 attttcagag ttttatataa aagcaattca ctgatttata tagcttattt gacatgcatc
    30121 gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca aagcgtatgg
    30181 cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca ggtataatcg
    30241 ccacaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat caacagacgc
    30301 attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga attgaagtgg
    30361 cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg actaaccaga
    30421 aaagcctggc tcgtacctca aaaaccccag ggcgcaccca gcttatcaac ctgtttgaag
    30481 tggctgacgg caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa gtcccggaag
    30541 agatgaagcg caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt cagagcctgc
    30601 aaggtctggt ggtgctaatg gatattcgcc atccgctgaa agatttggat cagcagatga
    30661 ttgagtgggc ggtagacagc aatatcgccg ttctggtgct gctgaccaaa gcggacaaac
    30721 tggcaagcgg cgcacgtaaa gcgcaattga atatggtgcg tgaagctgta ctggcgttta
    30781 acggtgatgt gcaggttgaa acgttttctt cgttgaagaa acaaggcgtg gacaagctgc
    30841 ggcagaaact ggatacctgg tttagcgaga tgcagcctgt agaagaaacg caggacggcg
    30901 aataattttc ttgccttaat gcttgtgccg gatgtggcgt atccggcccg taaattcaat
    30961 agattacaat tctaccgttg gcctgataaa catagcgcat caggcattac ggatcttttc
    31021 tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa tattcagcca
    31081 aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct gtaccctacg
    31141 cgactaactt tactcttttt tgttcagttc agaaagcact ttttgtaatt ttttttcagt
    31201 tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt gcttacttac
    31261 gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat cccagttttc gccactcccc
    31321 acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg atgaatctgc
    31381 ttcgcgacgg catcaacatc atctttatga acttcaaata ccagttcatc gtgtacctgc
    31441 atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc aatcatcgcc
    31501 cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc acgttcagcc
    31561 gctgcacgac gagcaccatt gctggatttg atatccggca gatacagacg gcgtccgtcc
    31621 agcgtttcaa cgtagccctg ctctttcgcc tgagcacggg tgcgttccat atactccagc
    31681 acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt acgtggaatg
    31741 ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa gttgatcgct
    31801 ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg gcaaactaaa cacttctgcc
    31861 gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc tttgtcacgc
    31921 gaaagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgctgagac aatcacataa
    31981 tcctctggcg caataaacgc ctggcggata cgacgacctt cttcgttacg caccggaatg
    32041 ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc ctggtgataa
    32101 gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc ggtgtaggtc
    32161 gatttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg atagtccagc
    32221 gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt tttcttcagc
    32281 ggtttaatac cctgtttttc aaagagaatg gtttgcaact gcttggtgga agaaaggtta
    32341 aattcttcac ctgcaatttc atgcgctttc ttttccagct cagccagacg caaggtgagc
    32401 tcttcagaat gattgtgcag cactttcgga tcgatcttca caccgttacg ttcaatgcgt
    32461 gaaagcaccg gcaccagcgg catttcgata ttctcgaaga cgttcaacgg ccctttgtgt
    32521 ttttgcagat ctggccacat tttcagatgc aactgcaagg tgacatcagc atcttcggcg
    32581 gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg tcagttgatt tttgccttta
    32641 cccgcaatct cttcaaaagt gatggttttg tgcttcaacc aacgttccgc gaggctgtcc
    32701 atatcgtgac gcccggcaac gctattgaga atgtaggact ccagcatggt atcaaacgca
    32761 atcccacgca gctcaatgcc gtaattcgcc agaataccgc gatcgtattt caggttttgc
    32821 ccgaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag tgcacgctcg
    32881 cgagagattt gatcgggcgc gtcaagataa tcatgagcaa ccggaatata tgccgctacg
    32941 cctggctcga tagcaaaaga aagcccgacc aggttagcag agatgttatc aaggctgtcg
    33001 gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca cgctttcagt
    33061 gtttcttcat caaggatggt gacgtagttg tcataagaaa tcaccgttgc cgtcacttct
    33121 ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg ctggttttgc ccctttggcc
    33181 tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata ctttttgaac
    33241 agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcaca ggtcagctcc
    33301 agttcaacgt cggttttaat cgtcgccagc tggtatgaga gataagcaac ttctttgttt
    33361 tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccagc aattttttct
    33421 ggctcggcat acagcgtatc cagcccgcca agaccttgca gcaatgcctg cgcggttttt
    33481 tcaccgacgc ccggtacgcc aggaatgtta tcggaggagt cacccatcag cgccaggaaa
    33541 tcgatgatca gttctggcgg cacgccgtac ttattcacca cctcttccgg tccgaggatg
    33601 gtattcgtca tggtattgat aagcgtaata tttggcgtca ccagctgcgc catatcttta
    33661 tcgccagtgc tgatcagcac cggacgcccg gctttttcgg cttcgcgcgc cagagtaccg
    33721 ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat cgctttaacc
    33781 atcgcgtgca ggggttcgat ttgtgctcgc agatcgttcg gcattggcgg gcgatgtgat
    33841 ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa gaccaccgct
    33901 gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac accatacatc
    33961 gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa acgcgtgata tgcgcgataa
    34021 agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac cataatgtcc
    34081 gtgcctgttt atcagattat gggtaaagga tgccacagac aggatgaaaa catgagtttt
    34141 tcaccgcttt tcgtgaaaat cttttgtaga tcttctggat cgctcgcaaa tcgtcatgtg
    34201 gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc tttttacagc
    34261 atgatatttg tctttgtttt tcatgcagtt aaaaataaag ccatccctaa taattgagat
    34321 aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag gatcatccgt
    34381 tatttgggtc gttttctgct aaaatctgcg ccttgcgctt ctttagcgca ctgtctatgg
    34441 ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc cttttgctga
    34501 gtatcgtatt gaccattttg gtcactattt tttgttccgt cccgatcatc attgccggga
    34561 ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt ttttgtgatt
    34621 ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac ccacacctgc
    34681 aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg cttatttgta
    34741 accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag cacattccga
    34801 tgaataaata tttcctcaag caacagctgg cctgggtgcc atttcttggc ctggcgtgct
    34861 gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta cgtcatccgg
    34921 agcgacgtgg taaagatgtt gaaaccactc ggcgttcttg tgaaaagttt cgcctgcatc
    34981 ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa catcagcaaa
    35041 cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg atggcgctaa
    35101 atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat ccggacaata
    35161 accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcgaattgtc gttcatgtgg
    35221 atttacagcc tattgccgat gagttacatg gcgactacat caatgacaaa agctttaagc
    35281 gccattttca gcaatggctg aactcactgt ggcaggaaaa agaccaactt ctgacgtcgt
    35341 tgatgtcatc acagcggcag gacaagtaaa ttttgctcgc ctctgaatga gcagaggcga
    35401 gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt cgctcatctc
    35461 ctgtccgtta aaaacaactt tacccggtgt ataacgcagt tgcagcgagg cggtattgtt
    35521 gtcaatcgtg attaactgaa acatctgacc taaggtcatc atcccgctga tttgtttatc
    35581 agcccgcttt tgcgcttttt ccgcatccat tccttcagat aaattaagct gttttgcggt
    35641 ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttaa tatctttgtt
    35701 tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg catttagttc
    35761 gccgagtgcg ttcttccatt ttaccggttg tttaatggtc ggctcacttt tttgtaataa
    35821 cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt catcgtttgc
    35881 taactcaggg tgtgcagcaa gctgcttctg cattgcaatg ttatactgga tgataaattg
    35941 gcgcaccgct gagggatcaa tagattcagc aatcaatgac cactcaccac taccgagttg
    36001 ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga tataatcttt
    36061 gtctttaacg cgatccagca tggtttttgc aacgaaagca tcgatttggg caacgtcttc
    36121 cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa ccggaaattt
    36181 cttttcttct aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta ctttgttcat
    36241 tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt tagacaatga
    36301 actaccatca ccattaaaag taaactggcc ctcaccaaaa gaaaactttc catactctgc
    36361 aggggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca atgtcgcttc
    36421 cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat tattcactaa
    36481 gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca ttgtgatggg
    36541 caacggaccg tgctcaacat ccacatcaaa tacaactttc tggcctggct tgatattgag
    36601 atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa aaaagccttt
    36661 gtcaaagttt ttataactta acgtcatatc gtaggcatgt tcacctttct ttttcgcatc
    36721 gttaaaatct ttaataaact tttcgatacc aggctgaatt tgcgtacctg tgtaccatgt
    36781 gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact tacgtatcat
    36841 ataaatattc cctgataaaa cagcaaaact ttgaatatcc acttatgctg aaataatgtc
    36901 cagcggcagg atgcattatc agaatgtatt tatatttatt aaataataaa aaaagcccgt
    36961 gaatattcac gggccttatg taatttacat tgaattattt tttctcggac agatatttca
    37021 ctgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata ccctgcggat
    37081 tcagctgata tttaccgtta acaaacatcg ccggaacgcc acgcaattgc acgtcagctg
    37141 cagctttttc ctgctgagcg accagagatt tgaccacgaa gctgttccac gccgcgtcgt
    37201 actcttcacc tttaatacct gcgttgataa atacatcgcg gatatcagat gctgaacgaa
    37261 tggtctgggt tttctgtacg ccttcaaaca gcggaacagt gactttgtct tccacgccca
    37321 gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca cccatgaagt
    37381 tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca ttatcagaaa
    37441 tatgcagaac ttcttcaaac tgatagcagt gcggacagaa gaaagagaaa aactccagca
    37501 cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca tcttcatact
    37561 gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa atctttttca
    37621 tgatctactc tctccgatta atacataggt gttaattgta aagggggttc ttgtagaact
    37681 tttgcctgtt ctataaaagt cgccgtttgt cgcagccagt aatcttcccc ggttaaccac
    37741 gggaaatttt tcgggaacgc gggatcagcc caacgccgca ttagccaggc aagataataa
    37801 accaaacgca tggcgcgtaa aggttcaatc agtccgattt cagcggtgtc gaactcgcta
    37861 aattcttcat aagcttcaat aatagtttcc agttgcatcc gctgctcggc tttatcgcca
    37921 ttgagcaaca tccacaaatc ctgaatagct ggaccattac gtgcatcatc cagatcaaca
    37981 aacattggac catcgcgcca gagaatattc ccggcgtggc agtctccatg tagccgcaga
    38041 acggtgaaat cttcccgcca gtgcgctgta acggcggcga tcagctcatc tgtcgctttc
    38101 aggaatgccg ctttcaaccc ggaaggtatc agtgtagcgt cctcaaacag cttgcgtggc
    38161 tcaatgagat attcattcag accgatggtc gggcgatgga taaaaagctg tttgcgcccc
    38221 gtctggtgca tacgccctaa ataacgccca accgcttcca tctgatcgat attatcagct
    38281 tcgaactggc gaccaccgac gcttggaaaa acagcgaaat aaaatccctg atgattcaat
    38341 aaagtctgac cgttaaaggc cacaggtgct gcgaccggaa cttcatcatt taccaactgc
    38401 aacgcaaatt gatgttcttc gaggatttga tcggctgtcc aacgttcagg gcgataaaat
    38461 ttgacgacaa aacgtcgacg atcttcgtcc tgaaattgat agacacggtt ttcatagctg
    38521 ttaagcgggg taagaccgga atccacccgg atcccttgct caaatagagc atccatgatg
    38581 gtatccgggt gtagtgtctg gaaagtaaaa gcgctgttgt tcatcccatc atccggaaaa
    38641 cattacgaat gatgaaggat atcatttcac gatgattttg gtggcaccgc ttacaagctt
    38701 ttactcttta attacgccgc gagcgcgtaa cagcgccgtt ttgaagtctt cttcataatc
    38761 tttctgcaaa cccgggatca ccgcatcttt cgcggaatca cgcattttca ggtgatagat
    38821 cagaatgtca tccgtcaaat ccgccagttc gccgtcaaaa cctgactctt tcgccagttt
    38881 ctgcaaaaat tgcaacaggt taaggtctgg ctctttttgc caggctggct ggaggagttc
    38941 aataacttca ttcagacgtt tacatttcat agtaatgctc cttactcttg agacagacac
    39001 gttagcaggg tcaatcccac aataaaagag gcgatatcgg tgaatctgat gacgacgata
    39061 acaggcgttg tgctggcagg cggtaaagcc agacgaatgg gcggcgtaga taaaggattg
    39121 cttgaattaa acggcaaacc attatggcaa catgtcgctg acgcgcttat gacgcagctc
    39181 tctcacgtcg tggttaatgc caatcgccat caggaaatct atcaggcaag cggtctgaaa
    39241 gtgattgaag attcactggc ggattaccca ggccctctgg caggaatgct ttcagtaatg
    39301 cagcaggaag caggcgagtg gtttttgttt tgtccgtgcg atactcctta tattcccgag
    39361 gatttagcag cccggcttaa tcatcagcgc aaagatgcgc ctgtcgtgtg ggtccacgac
    39421 ggtgaacgcg atcacccgac tattgctctg gtaaaccgcg caattgagcc tttattactg
    39481 gaatatctgc aagcaggaga acgccgggta atggcattta tgcgtctggc tggcggtcat
    39541 gcggttgatt tcagcgataa taaagatgca tttattaacg tgaatacgcc agaggagctt
    39601 gcccgatggc aggaaaaacg atgataccgt tactcgcctt tgccgcgtgg agtggtaccg
    39661 gaaaaactac gcttttgaaa caactgatcc cggcattatg cgccagaggg atccgtccag
    39721 ggctgattaa gcatacgcac catgatatgg atgttgataa gccaggcaaa gatagctatg
    39781 agctgcgcaa ggctggcgcg gcacaaacca tcgttgccag ccagcaacga tgggccttga
    39841 tgacggaaac accagacgaa gaagagctgg atctacagtt tctcgcaagt cgaatggata
    39901 cctcaaagct ggatttgatt ctggtcgaag ggtttaagca tgaagagatc gcaaagattg
    39961 tgctgtttcg cgatggagcc ggacatcgac ctgaagaatt agtgatagac aggcatgtta
    40021 ttgctgtagc cagtgatgtg ccgcttaatc ttgatgtcgc attactggat atcaatgatg
    40081 ttgaggggct ggctgatttt gtggtggagt ggatgcaaaa gcagaacgga taagcaagag
    40141 taggctggat aagacgcgcc agcgtcgcat ccggcatttt tttctgcgcc aa
//
LOCUS       DAZFWL020000027        39813 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFWL020000027 DAZFWL020000000
VERSION     DAZFWL020000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.027"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            70..1125
                     /gene="phoE"
                     /locus_tag="ACHKNP_RS07765"
                     /old_locus_tag="pgaptmp_001553"
     CDS             70..1125
                     /gene="phoE"
                     /locus_tag="ACHKNP_RS07765"
                     /old_locus_tag="pgaptmp_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGU4703747.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNFYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1164..1565)
                     /gene="crl"
                     /locus_tag="ACHKNP_RS07770"
                     /old_locus_tag="pgaptmp_001554"
     CDS             complement(1164..1565)
                     /gene="crl"
                     /locus_tag="ACHKNP_RS07770"
                     /old_locus_tag="pgaptmp_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU4703748.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1623..2867)
                     /gene="frsA"
                     /locus_tag="ACHKNP_RS07775"
                     /old_locus_tag="pgaptmp_001555"
     CDS             complement(1623..2867)
                     /gene="frsA"
                     /locus_tag="ACHKNP_RS07775"
                     /old_locus_tag="pgaptmp_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU4703749.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2959..3417)
                     /gene="gpt"
                     /locus_tag="ACHKNP_RS07780"
                     /old_locus_tag="pgaptmp_001556"
     CDS             complement(2959..3417)
                     /gene="gpt"
                     /locus_tag="ACHKNP_RS07780"
                     /old_locus_tag="pgaptmp_001556"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU4703750.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3678..5135
                     /gene="pepD"
                     /locus_tag="ACHKNP_RS07785"
                     /old_locus_tag="pgaptmp_001557"
     CDS             3678..5135
                     /gene="pepD"
                     /locus_tag="ACHKNP_RS07785"
                     /old_locus_tag="pgaptmp_001557"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU4703751.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5192..5806)
                     /gene="prfH"
                     /locus_tag="ACHKNP_RS07790"
                     /old_locus_tag="pgaptmp_001558"
     CDS             complement(5192..5806)
                     /gene="prfH"
                     /locus_tag="ACHKNP_RS07790"
                     /old_locus_tag="pgaptmp_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HGU4703752.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5803..6942)
                     /locus_tag="ACHKNP_RS07795"
                     /old_locus_tag="pgaptmp_001559"
     CDS             complement(5803..6942)
                     /locus_tag="ACHKNP_RS07795"
                     /old_locus_tag="pgaptmp_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HGU4703753.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7188..7640)
                     /locus_tag="ACHKNP_RS07800"
                     /old_locus_tag="pgaptmp_001560"
     CDS             complement(7188..7640)
                     /locus_tag="ACHKNP_RS07800"
                     /old_locus_tag="pgaptmp_001560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4703754.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(7637..8692)
                     /gene="dinB"
                     /locus_tag="ACHKNP_RS07805"
                     /old_locus_tag="pgaptmp_001561"
     CDS             complement(7637..8692)
                     /gene="dinB"
                     /locus_tag="ACHKNP_RS07805"
                     /old_locus_tag="pgaptmp_001561"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGU4703755.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8763..9686)
                     /gene="lafU"
                     /locus_tag="ACHKNP_RS07810"
                     /old_locus_tag="pgaptmp_001562"
     CDS             complement(8763..9686)
                     /gene="lafU"
                     /locus_tag="ACHKNP_RS07810"
                     /old_locus_tag="pgaptmp_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HGU4703756.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(9689..10552)
                     /gene="motA"
                     /locus_tag="ACHKNP_RS07815"
                     /old_locus_tag="pgaptmp_001563"
     CDS             complement(9689..10552)
                     /gene="motA"
                     /locus_tag="ACHKNP_RS07815"
                     /old_locus_tag="pgaptmp_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HGU4703757.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(10565..11281)
                     /locus_tag="ACHKNP_RS07820"
                     /old_locus_tag="pgaptmp_001564"
     CDS             complement(10565..11281)
                     /locus_tag="ACHKNP_RS07820"
                     /old_locus_tag="pgaptmp_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HGU4703758.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLVGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(11301..11768)
                     /locus_tag="ACHKNP_RS07825"
                     /old_locus_tag="pgaptmp_001565"
     CDS             complement(11301..11768)
                     /locus_tag="ACHKNP_RS07825"
                     /old_locus_tag="pgaptmp_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HGU4703759.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(11776..12837)
                     /locus_tag="ACHKNP_RS07830"
                     /old_locus_tag="pgaptmp_001566"
     CDS             complement(11776..12837)
                     /locus_tag="ACHKNP_RS07830"
                     /old_locus_tag="pgaptmp_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGU4703760.1"
                     /translation="MNPTLLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKSELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(12834..13145)
                     /locus_tag="ACHKNP_RS07835"
                     /old_locus_tag="pgaptmp_001567"
     CDS             complement(12834..13145)
                     /locus_tag="ACHKNP_RS07835"
                     /old_locus_tag="pgaptmp_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HGU4703761.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(13150..13542)
                     /gene="fliS"
                     /locus_tag="ACHKNP_RS07840"
                     /old_locus_tag="pgaptmp_001568"
     CDS             complement(13150..13542)
                     /gene="fliS"
                     /locus_tag="ACHKNP_RS07840"
                     /old_locus_tag="pgaptmp_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGU4703762.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(13565..14881)
                     /gene="fliD"
                     /locus_tag="ACHKNP_RS07845"
                     /old_locus_tag="pgaptmp_001569"
     CDS             complement(13565..14881)
                     /gene="fliD"
                     /locus_tag="ACHKNP_RS07845"
                     /old_locus_tag="pgaptmp_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGU4703763.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSASVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(15088..16002)
                     /gene="lafA"
                     /locus_tag="ACHKNP_RS07850"
                     /old_locus_tag="pgaptmp_001570"
     CDS             complement(15088..16002)
                     /gene="lafA"
                     /locus_tag="ACHKNP_RS07850"
                     /old_locus_tag="pgaptmp_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HGU4703764.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            16488..17330
                     /locus_tag="ACHKNP_RS07855"
                     /old_locus_tag="pgaptmp_001571"
     CDS             16488..17330
                     /locus_tag="ACHKNP_RS07855"
                     /old_locus_tag="pgaptmp_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HGU4703765.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLEKLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPDSSPAKKLIITDQIQETVPEEISSTTPPVPVRKKHKVIMGLALAAAVIFIGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQTLAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     LQKC"
     gene            complement(17385..18362)
                     /locus_tag="ACHKNP_RS07860"
                     /old_locus_tag="pgaptmp_001572"
     CDS             complement(17385..18362)
                     /locus_tag="ACHKNP_RS07860"
                     /old_locus_tag="pgaptmp_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HGU4703766.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQNTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAAQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPHIERTLSVRTDGDK
                     SAFMPLKTFAEPSEADRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(18379..19308)
                     /gene="flgL"
                     /locus_tag="ACHKNP_RS07865"
                     /old_locus_tag="pgaptmp_001573"
     CDS             complement(18379..19308)
                     /gene="flgL"
                     /locus_tag="ACHKNP_RS07865"
                     /old_locus_tag="pgaptmp_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HGU4703767.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKQILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEVTLKTYSKVSELNLFSVL"
     gene            complement(19323..20699)
                     /gene="flgK"
                     /locus_tag="ACHKNP_RS07870"
                     /old_locus_tag="pgaptmp_001574"
     CDS             complement(19323..20699)
                     /gene="flgK"
                     /locus_tag="ACHKNP_RS07870"
                     /old_locus_tag="pgaptmp_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HGU4703768.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGDLSQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTSTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PNITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(20881..21180)
                     /locus_tag="ACHKNP_RS07875"
                     /old_locus_tag="pgaptmp_001575"
     CDS             complement(20881..21180)
                     /locus_tag="ACHKNP_RS07875"
                     /old_locus_tag="pgaptmp_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HGU4703769.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            complement(21180..22280)
                     /locus_tag="ACHKNP_RS07880"
                     /old_locus_tag="pgaptmp_001576"
     CDS             complement(21180..22280)
                     /locus_tag="ACHKNP_RS07880"
                     /old_locus_tag="pgaptmp_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HGU4703770.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(22295..22960)
                     /gene="flgH"
                     /locus_tag="ACHKNP_RS07885"
                     /old_locus_tag="pgaptmp_001577"
     CDS             complement(22295..22960)
                     /gene="flgH"
                     /locus_tag="ACHKNP_RS07885"
                     /old_locus_tag="pgaptmp_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HGU4703771.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FSHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADACISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(23043..23828)
                     /gene="flgG"
                     /locus_tag="ACHKNP_RS07890"
                     /old_locus_tag="pgaptmp_001578"
     CDS             complement(23043..23828)
                     /gene="flgG"
                     /locus_tag="ACHKNP_RS07890"
                     /old_locus_tag="pgaptmp_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGU4703772.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(23907..24644)
                     /locus_tag="ACHKNP_RS07895"
                     /old_locus_tag="pgaptmp_001579"
     CDS             complement(23907..24644)
                     /locus_tag="ACHKNP_RS07895"
                     /old_locus_tag="pgaptmp_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HGU4703773.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLEKNAEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(24644..25846)
                     /gene="flgE"
                     /locus_tag="ACHKNP_RS07900"
                     /old_locus_tag="pgaptmp_001580"
     CDS             complement(24644..25846)
                     /gene="flgE"
                     /locus_tag="ACHKNP_RS07900"
                     /old_locus_tag="pgaptmp_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HGU4703774.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVMCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFNASD
                     EAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPVDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(25870..26574)
                     /locus_tag="ACHKNP_RS07905"
                     /old_locus_tag="pgaptmp_001581"
     CDS             complement(25870..26574)
                     /locus_tag="ACHKNP_RS07905"
                     /old_locus_tag="pgaptmp_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HGU4703775.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGDDIM
                     VQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQVDFS
                     VNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGEVPF
                     TMISQFGVPDADSDVA"
     gene            complement(26574..27005)
                     /gene="flgC"
                     /locus_tag="ACHKNP_RS07910"
                     /old_locus_tag="pgaptmp_001582"
     CDS             complement(26574..27005)
                     /gene="flgC"
                     /locus_tag="ACHKNP_RS07910"
                     /old_locus_tag="pgaptmp_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HGU4703776.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(27008..27343)
                     /locus_tag="ACHKNP_RS07915"
                     /old_locus_tag="pgaptmp_001583"
     CDS             complement(27008..27343)
                     /locus_tag="ACHKNP_RS07915"
                     /old_locus_tag="pgaptmp_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HGU4703777.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            27425..28162
                     /gene="flgA"
                     /locus_tag="ACHKNP_RS07920"
                     /old_locus_tag="pgaptmp_001584"
     CDS             27425..28162
                     /gene="flgA"
                     /locus_tag="ACHKNP_RS07920"
                     /old_locus_tag="pgaptmp_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HGU4703778.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMTKSLVARDMPLTANDVELKPYNVSAQRRDVLMELDDAI
                     GLSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            28243..28521
                     /gene="flgM"
                     /locus_tag="ACHKNP_RS07925"
                     /old_locus_tag="pgaptmp_001585"
     CDS             28243..28521
                     /gene="flgM"
                     /locus_tag="ACHKNP_RS07925"
                     /old_locus_tag="pgaptmp_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGU4703779.1"
                     /translation="MKITPTMPGNRPTATTAGSSQPRKTTVTTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSNKVAQVQAMLASGSPQVDTEQLASDMLSFFQN"
     gene            28534..28962
                     /gene="flgN"
                     /locus_tag="ACHKNP_RS07930"
                     /old_locus_tag="pgaptmp_001586"
     CDS             28534..28962
                     /gene="flgN"
                     /locus_tag="ACHKNP_RS07930"
                     /old_locus_tag="pgaptmp_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HGU4703780.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEEL
                     LAVNDEITHHYEQLHGHSHQRHSLLKMLGVSVNRDGLAQVFTWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQSFLGSEADFLYQE"
     gene            29089..30018
                     /locus_tag="ACHKNP_RS07935"
                     /old_locus_tag="pgaptmp_001587"
     CDS             29089..30018
                     /locus_tag="ACHKNP_RS07935"
                     /old_locus_tag="pgaptmp_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HGU4703781.1"
                     /translation="MSFIECYEPEYVRKFLARHPGSPLYVRKVWKNEIRLSLSLTDIA
                     SCEAVLNDVRAFDLQLTYRPVEDNSAELSARDAIVNQVILSTLTLRDLTPELSLYAVG
                     ILLSCASKMPGRDGDILARLTTLPQALADHAKKGTLQAQYAQLPPVPQLARQLMTLLG
                     GCAFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLQTQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDMIGQTDGADYFPLVCDFYLLRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENALLVRQQIQSLCAADPLLSAFSLLT"
     gene            30197..32641
                     /locus_tag="ACHKNP_RS07940"
                     /old_locus_tag="pgaptmp_001588"
     CDS             30197..32641
                     /locus_tag="ACHKNP_RS07940"
                     /old_locus_tag="pgaptmp_001588"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU4703782.1"
                     /translation="MPTPTIIKTLATHREKPFVSVVTPTWNRGAFLPYLLYMYRYQDY
                     PADRRELIILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELARG
                     EYILCMDDDDYYPADKISYTIAMMQKHRALISGSDQIPIWYSHINRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFIIGASTPVNTALTDIVRDPLLRNAYLSLHNATHHQAINHQAIDQVVVVNLDKRPDR
                     LQQIREELALLHIPPEKITRLAASEDENGQRGRRQSHLQALRLAQQHGWQNYLLLEDD
                     AVILKQEKHIQVLNTLLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNSRYYPQLAQQMSNDEHSLEDCWQPLLRADKWLACYPSLCYQRPGFSDIQKKTTDN
                     IARYFNKLPVTTKPSALPIADTIGFFMETSFHYALYRPIITALQAQGLSCTLVINDRV
                     FKPFLDEMLETLKNIDDPQLKGMRLSEMQTHGQRVKCLVSPYHTPALNGLAAVNIRAM
                     YGLAKETWNHADWNRFYQSILCYSHYSQQALAHFGSAKVVGNPRFDAWHNGTFDRALP
                     ENIQSDYRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTCSRPEEAASL
                     ALVRRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMNDLLD
                     GEKSYSTAESADQRIREILPVAHDVAELRYLLSEAFDWGSVQARLTEIRHHYCDAFMD
                     GKAGERAAIVIVEALG"
     gene            complement(32759..33154)
                     /locus_tag="ACHKNP_RS07945"
                     /old_locus_tag="pgaptmp_001589"
     CDS             complement(32759..33154)
                     /locus_tag="ACHKNP_RS07945"
                     /old_locus_tag="pgaptmp_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HGU4703783.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARALGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDS
                     FAYICEVVYFPRTPSVSTTGIIEVIRGLL"
     gene            complement(33157..33591)
                     /gene="fliJ"
                     /locus_tag="ACHKNP_RS07950"
                     /old_locus_tag="pgaptmp_001590"
     CDS             complement(33157..33591)
                     /gene="fliJ"
                     /locus_tag="ACHKNP_RS07950"
                     /old_locus_tag="pgaptmp_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HGU4703784.1"
                     /translation="MRQIIDTLTQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAVSVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(33594..34931)
                     /gene="fliI"
                     /locus_tag="ACHKNP_RS07955"
                     /old_locus_tag="pgaptmp_001591"
     CDS             complement(33594..34931)
                     /gene="fliI"
                     /locus_tag="ACHKNP_RS07955"
                     /old_locus_tag="pgaptmp_001591"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGU4703785.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ"
     gene            complement(34924..35634)
                     /gene="fliH"
                     /locus_tag="ACHKNP_RS07960"
                     /old_locus_tag="pgaptmp_001592"
     CDS             complement(34924..35634)
                     /gene="fliH"
                     /locus_tag="ACHKNP_RS07960"
                     /old_locus_tag="pgaptmp_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HGU4703786.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTTSISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALKETLTPESQGE"
     gene            complement(35637..36647)
                     /locus_tag="ACHKNP_RS07965"
                     /old_locus_tag="pgaptmp_001593"
     CDS             complement(35637..36647)
                     /locus_tag="ACHKNP_RS07965"
                     /old_locus_tag="pgaptmp_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGU4703787.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVGRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            complement(36625..38271)
                     /gene="fliF"
                     /locus_tag="ACHKNP_RS07970"
                     /old_locus_tag="pgaptmp_001594"
     CDS             complement(36625..38271)
                     /gene="fliF"
                     /locus_tag="ACHKNP_RS07970"
                     /old_locus_tag="pgaptmp_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HGU4703788.1"
                     /translation="MNAQIKKLTLAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEEALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTHASVANVLDSLVGSGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPVAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTAQ
                     LTALLNNAAGIDAQRGDNLTLSLLNFVPQAVPVEPIIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPVMKRLTAVRAPIITPESEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(38276..38617)
                     /locus_tag="ACHKNP_RS07975"
                     /old_locus_tag="pgaptmp_001595"
     CDS             complement(38276..38617)
                     /locus_tag="ACHKNP_RS07975"
                     /old_locus_tag="pgaptmp_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGU4703789.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     HRIMQGALGHVDQFQQVAEQQQTAIDMGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(38632..39618)
                     /locus_tag="ACHKNP_RS07980"
                     /old_locus_tag="pgaptmp_001596"
     CDS             complement(38632..39618)
                     /locus_tag="ACHKNP_RS07980"
                     /old_locus_tag="pgaptmp_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HGU4703790.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENENAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRAMLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPENDIADHSELQWPAQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKHGIEVFS"
BASE COUNT         8632 a        11113 c        10488 g         9580 t
ORIGIN      
        1 aaaactttcc tgttttcaac ggggctctcc cgctgaatat tcgcgcgtta attaaaatca
       61 ggaatgaaaa tgaaaaagag cactctggca ttagtggtga tgggcattgt ggcatctgca
      121 tctgtacagg ctgcagaaat atataataaa gacggtaata aactggatgt ctatggcaaa
      181 gttaaagcca tgcattatat gagtgataac gccagtaaag atggcgacca gagttatatc
      241 cgttttggtt ttaaaggtga aacacaaatt aacgatcaac tgactggtta tggtcggtgg
      301 gaagcggagt ttgccggaaa taaagcggag agtgataccg cacagcaaaa aacgcgtctc
      361 gcttttgccg ggttgaaata taaagatttg ggttctttcg attatggtcg taacctgggc
      421 gcgttgtatg acgtggaagc ctggaccgat atgttcccgg aatttggtgg cgattcctcg
      481 gcgcagaccg acaactttat gaccaaacgc gccagcggtc tggcgacgta tcggaacacc
      541 gacttcttcg gcgttatcga tggcctgaac ttaaccctgc aatatcaagg gaaaaacgaa
      601 aaccgcgacg ttaaaaagca aaacggcgat ggcttcggca cgtcattgac atatgacttt
      661 ggcggcagcg atttcgccat tagtggggcc tataccaact cagatcgcac caacgagcag
      721 aacctgcaaa gccgtggcac aggcaagcgt gcagaagcat gggctacagg tctgaaatac
      781 gatgccaata atttttatct ggcaactttt tattctgaaa cacgcaaaat gacgccaata
      841 actggcggct ttgccaataa gacacagaac tttgaagcgg tcgctcaata ccagtttgac
      901 tttggtctgc gtccatcgct gggttatgtc ttatcgaaag ggaaagatat tgaaggtatc
      961 ggtgatgaag atctggtcaa ttatatcgat gtcggggcta catattattt caacaaaaat
     1021 atgtcagcgt ttgttgatta taaaatcaac caactggata gcgataacaa attgaatatt
     1081 aataatgatg atattgtcgc ggttggcatg acctatcagt tttaatgatt attgccggat
     1141 gtgatgcatc cggcacattt cactcacgcc gttaacttca ccggctcgtc acgaaaatca
     1201 tccgccggtt ccagcttcag attcagcgtc gtcagcagtt cacgcagctt ctcgtgaaac
     1261 tcacgcaggg tgtgctccag tcgttcaacc acttcagtgt cttttaccgg aacactcttc
     1321 cagtcgcctg ctttatcgaa cagaccaaac tggtaactgt aggtaaaacg ggattcctgc
     1381 gcttcaagct ccatccacca gccccagaac tcacgcactt ccggtgccgg tttcacgttg
     1441 acgcatacag ccagacaatc gaaaaagaat cgattatctt cgcacttacc ttcacgaata
     1501 tacgggccta gtgcggtaaa ttttttgatc aatctgctct tcgggtgtcc actcggtaac
     1561 gtcattgcta tctcctgttg tgatgcaact gttttaccaa attggcaaaa tttagcaaat
     1621 ttttaacaca agcgtttttc gatccagccg gtgatttcct gaagaccttt gtcaaaattc
     1681 cgatacaccg ggttaaatgg gatctctaat aatttaccgt cagcagatga tgaggtgatt
     1741 aagcgtgagt cctcttccgg gctgaacgga tcgttcttcc agtagcctga taacattggc
     1801 gttgggcagc gacgccccag caatccttgc acttttaatg aatagcgatt cagctccacg
     1861 cgtaacgcgt catcggaagc atcatgcatc cccaaacgac tcgccagaac gtcaagatac
     1921 atttccggca cctgttgctg gcacttaaaa tcactcaaca gggtatgaac taccggacca
     1981 agacaggcaa ccgctttcag acgcggcgat tcaagatatg ccagacgcac ggcaacgtta
     2041 gcgccgaaac ggaaaccaaa ggccgcgacg cgagtgtgat ccacccacgg tacgttaggc
     2101 agcgccttta agacgtgctg atgcaacagg ctggagtcct gggtgagctt ccattttgaa
     2161 gaaaagccca ccgatggcat atcaatagtc agcatcgcaa tgccgcgcgg cgcaaaataa
     2221 cgttcataca ggctgtaata gtccgtctgc atcgcatcca gaccaccaca cattaatact
     2281 gtcgggaacg ggccatcgcc tttcggcata tgcaaaaagc cggtgatggg cgcaccgccg
     2341 ggtacggtaa actccatctg ccgcatcgtg cccggcagac gctgggcggc ctcttcatag
     2401 gcgcggtttg ataaagcctg cgcttgctcg gccaggtcat ctcctttcag atgaggatag
     2461 gcggcaatgt tgtacaacgt agccgcatgc agccagtgtc gaccactgag ttgcggatct
     2521 tcttccgcac aggctttttg ttgccacacc atcgcctggg tggcccactc ataaatccag
     2581 ttgccaccac ggtagccaat cacagtatcg tataaatcat cgtcggtacg ttcagcatcg
     2641 ctcatcacaa tacgtgcctg gacgtcgagg atttcacgtg ggtcaatacc gcgccagatc
     2701 cacatcagac ggttaatcat gcgataccag tgagggatgg ttttaccatc aagagccgac
     2761 tgcacaggcg gttgtgcgcc gtgattaaag cggcggacta gcgtcgaggt ttcaggatgt
     2821 ttaaagcggg gtttgaacag ggtttcgctc aggtttgcct gtgtcatgga tgcagcctcc
     2881 agaatactta ctggaaacta ttgtaacctg cctgaagtta aaaagaacaa cgcccggcag
     2941 tgccaggcgt tgaaaagatt agcgaccgga gattggcggg acgaatacga cgcccatatc
     3001 ccacggctgt tcgatccagg tatcttgcgg gatatcaaca acatagtcat caaccagcgg
     3061 acgaccagcc ggttttgcga agatggtgac aaagtgcgct tttggataca tttcacgaat
     3121 cgcaacagca gtaccaccgg tatccaccag gtcatcaata acgatgaagc cttcgccatc
     3181 gccttctgcg cgtttcagca ctttaagctc gcgctggttg tcgtgatcgt agctggaaat
     3241 acaaacggta tcgacatgac gaatacccag ttcacgcgcc agtaacgcac ccggtaccag
     3301 accgccacgg cttacggcaa taatgccttt ccattgttca gaaggcatca gtcggcttgc
     3361 gagtttacgt gcatggatct gcaacatgtc ccaggtgacg atgtattttt cgctcatgtg
     3421 aagtgtccca gcctgtttat ctacggctta aaaagtgttc gaggggaaaa taggttgcgc
     3481 gagattatag agatctggcg cactaaaaac cagtatttca catgagtccg cgtcttttta
     3541 cgcactgcct ctccctgacg cgggataaag tggtattctc aaacatatct cgcaagcctg
     3601 tcttgtgttg acaacatttt ctgctaaccc tgtgacctgc aatactgttt tgcgggtgat
     3661 cgacaaggag acttaacgtg tctgaactgt ctcaattatc tccacagccg ctgtgggata
     3721 tttttgccaa aatctgttct attcctcacc cgtcctatca tgaagagcaa ctcgctgaat
     3781 acattgttgg ttgggcaaaa gagaaaggtt tccatgtcga acgcgatcag gtaggtaata
     3841 tcctgattcg taaacctgct accgcaggta tggaaaatcg taaaccggtc gtcttacagg
     3901 cccacctcga tatggtgccg cagaaaaata acgacaccgt gcatgacttc acgaaagatc
     3961 ctatccagcc ttatattgat ggcgaatggg ttaaagcgcg cggcaccacg ctgggtgcag
     4021 ataacggcat tggtatggcg tctgcgctgg cggttctggc tgacgaaaac gtggttcacg
     4081 gcccgctgga agtgctgctg accatgaccg aagaagccgg tatggacggt gcgttcggct
     4141 tacagagcaa ctggttgcag gctgatattc tgattaacac cgactccgaa gaagaaggtg
     4201 aaatctacat gggttgtgcg gggggtatcg acttcacctc caacctgcat ttagatcgtg
     4261 aagcggttcc agctggtttt gaaaccttca agttaacctt aaaaggtctg aaaggcggtc
     4321 actccggcgg tgaaatccac gttggcctgg gtaatgccaa caaactactg gtgcgcttcc
     4381 tggcgggtca tgcggaagaa ctggatctgc gccttatcga tttcaacggc ggcacactgc
     4441 gtaacgccat cccgcgtgaa gcctttgcga ccattgctgt cgcagctgat aaagtcgacg
     4501 tcctgaaatc tctggtgaat acctatcagg agatcctgaa aaacgagctg gcagagaaag
     4561 agaaaaatct ggccttgttg ctggactctg tagcgaacga taaagctgcc ctgattgcga
     4621 aatctcgcga tacctttatt cgtctgctga acgccacccc gaacggtgtg atccgcaatt
     4681 ccgacgtggc aaaaggtgtg gtcgaaacct ccctgaacgt cggtgtggtg accatgactg
     4741 acaataacgt agaaattcac tgcctgatcc gttcactgat cgacagcggt aaagactacg
     4801 tggtgagcat gctggattcg ctgggtaaac tagctggcgc gaaaaccgaa gcgaaaggcg
     4861 catatcctgg ctggcagccg gacgctaatt ctccggtgat gcatctggta cgtgaaacct
     4921 atcagcgtct gttcaacaaa acaccgaaca tccagattat ccacgcgggc ctggaatgtg
     4981 gtctgttcaa aaaaccgtat ccggaaatgg acatggtttc tatcgggcca actatcaccg
     5041 gtccacactc tccggatgag caagttcaca tcgaaagcgt aggtcattac tggacgctgc
     5101 tgactgaact gctgaaagaa attccggcga agtaattatt tgatttgctg ccggatggcg
     5161 tttaatcgcc ttccggcagt ttcatccttc attatccttc gataaaagcc atccctgtaa
     5221 atgtccgtct cgggttgcca cgttcaatct ggtgatggaa cattcgccgc agcgatttca
     5281 gcaccgcgct attttcctgt tgctgctgct ccagcttcca ggcaatcagc aatcgtgcca
     5341 gccgcttgtt agcatgctga ctacgctctg actgaacctt cacgctaata ccggatgcca
     5401 aatgcgtggc gcgtaccgcc gagtcggttt tattgacatg ttgaccgccc ggccccgacg
     5461 aacgcaacgt ctcataacgg attgcatccg attgttcctg ctcatcagcg gtaaaacgcc
     5521 caatgcccag aaaccagttt ttgcgcccat gatgaggccg atacggactc ggacaaatcc
     5581 actgaatagt gccacaccag ctttcgctta atgcccatgc gttatcgcca tccagagaaa
     5641 tcagcgccga acgcagcgtg tcagagtagt ggcccgtttc tgtttccagc accgttaccg
     5701 cgacgtcttg tcgggcagct tctttaatta gcctgtccag cgcctttttc actgcgagac
     5761 aacattcttc cggcccctgc gctgaggaga gttgtagcaa gatcatcctt ttttccctcc
     5821 actgtttttg agcgttagca ccggacgcag ccgcgcgaca ggaataatta aaccggcctg
     5881 caccagacat tgcaccacgc tttcagccga tttataggct tgtggcgctt cttcaaagat
     5941 gagttgttta tcgcgacaaa ttacccgact gccaagttca gtacgcgaga gttgcgtcgc
     6001 cgtgtatttc gctgccagac gccctttaca ctcggtgcgc ccccatttac gccctgcccc
     6061 atgcgccagc gagtggagcg ttttttcgtt cgcaacaggc tgtacaatcc aggagtaatc
     6121 accacgtgaa ccgggaataa tcaccagacc gttgtcatcc ggcgtggcac ctttacgatg
     6181 caaccagccc tgttgatcgc cgatttgaca cgcgctaaca aagttatgcg ccacatccag
     6241 aaccggactt cccgtggcct taacttgttg cattatgcgc aaagcgatca tctggcgatt
     6301 aatacacgca aacgccagcg catcgtcatg ttccgcaata taccgtagcg cgtcgtcact
     6361 gccttgaggc aaaccatgat gcgagaacga ggcaatatgc cgctgtaaaa tagactgccc
     6421 caccccccgc gagccgctat gaaccaacag ttgcagatgt tgtgcatcca gacctgccag
     6481 tgcaaacagt tcagcgttga tgatttgatc aacttgttgc agttcagcga aatgattgcc
     6541 gccaccgata gagccaagcg agctgcgcca cggatgctgt gcaaatgctg acggcaggtt
     6601 ttcctccagc cagctttctt cagcaacgtc atccagatct gataatcgct tttcaaactt
     6661 atcggcgttg tatttgcgag cgagaatatc tgtttgccat agcgccatac cgcagccgat
     6721 atcattgccg accagtgccg gataaaaacg accaacagag aagaacgctg cgccaatcgg
     6781 atagccgcgt ccgggatgta aatctggcat ccccactacg cgctgcatgt tgggtaaatt
     6841 tgccgtggtg tgtaattgtt ggatcgctaa actctcgatc cacaggtcat cagatgcgat
     6901 ggtaaatacc gcatcggata agggacgaat ataattgccc atataccatt ccgtaaatag
     6961 actcgtcata cttcaagttg catgtgttgc gacttgaatt attgagagta acaattaaaa
     7021 aatcaggaaa tggcagacga atccagggca agaaaaaaag aattaatcct ggaaaggtct
     7081 gcaaagagtc attgataaag gatttgtcat gatttacctc cttgcagttt gtggaataag
     7141 aaaacgcgca catactaggc ttgcgttatt ttcattgcaa gctggattta atgttgctgt
     7201 ttataacgca tataaaaatt aataaaccac tctccccgac attcaacgcg ttgctgctta
     7261 actgtctgaa aaccataacg ttcaaaaaag ggttttgcgg ttatgcttgc gtccaccgta
     7321 agttcggatt cagacttaat caaaggtttt aacaaagcgc tggcaacccc acggcgggtg
     7381 tattcagggt caacaaataa catatcgata tagtgttcaa cgcaggtaat aaagccgacc
     7441 agttttgcat taatgactgc aacccgcact tgtgatttcg cgagtttctc cttccagcga
     7501 gattcgtcaa tctgcgccca ggcggcaatt tgttgtggtg aataatgctg actggcagtc
     7561 atcgtaaccg ctctaatgaa aatagcgcat agttgctgaa aatcgccagg ctgatagtct
     7621 cttatttgta tgttattcat aatcccagca ccagttgtct ttccatttgc gggtcaagca
     7681 acgtcacatg cagccccacc agtcgcacac cgcgcccgcc gcggcgttca tcccaggttt
     7741 tacgcgcggt ggcgattaag tcagctttat tcagccgcgg ccagacgtgc tcctgagtgg
     7801 tttgctgaaa atcatcaaac tttaatttca ctccctgacg ggcgatcagt aaatcaggtt
     7861 ttacctttgc cagacggcgt tcaagttccg gatacagccg ctcgataatc gcttcacatt
     7921 cagaccagtg atgaatatct tccgccatcg tgcgttccac gccgacggat tttcgtaacc
     7981 gttcgctgtt aacgtcgcgc tcgtcaatcc cctgactacg ctcccacaaa atgcggccaa
     8041 atttgccaaa gcgtttaagc agcatcacca gatcacactt ttgtacatca ccgcaggtcc
     8101 gcagccccat cgcttccagt tttgccgccg agactttgcc gacgccgggg atttttgcca
     8161 gcggtaaggt ttgtaaaaat gccggaactt ctgccggcgt aatcacaaac tgaccgttgg
     8221 gtttattcat gtcggaggcg attttggcga gaaactttac tggtgccacg cccgcagacg
     8281 ctgtcagttg cagctcgctg aaaatcgtct ggcggatttc ctgggcgatg agggtcgcag
     8341 aaccgtggca atggacgcta tcggtgacgt cgagataagc ctcatccagt gacaacggtt
     8401 caatacgcga ggtgtagcgc gagaagattt cacggatatg atttgaggct tctttgtagg
     8461 cgtcaaagcg ccccggaagc aaggtgagat gcgggcataa tttgagcgcc atccctgtcg
     8521 gcatagcgct acgtacgcca aatttacgcg cgggataatt ggcggtgctg atcacccccc
     8581 gacgttcgcg gctgccgcca atagcaatag ggatatcgcg cagggcggga ttgtcgcgca
     8641 tctccaccgc tgcgaaaaag cagtccatat ccacatgaat gattttacgc attactcacc
     8701 tctcaacact ggtaaagtat acagtgattt cagggtttga gaaatgcgta aagattcagc
     8761 atttaacgcg ttcgctgcga aagcacctgc tgcttatcca gcttttgcac cagcggctgc
     8821 accactttat ccccatgctg accaaagtat tgatacagcg tatcggacgc acttttggtc
     8881 agcaccataa tctcaatgcg ccggttgccc gcgctttgcg gatttttgga atccagcagc
     8941 atctggtccg ccattgcgct tacctgcatc actttatctt ccggcattcc ggcctcttcc
     9001 agcacccgac gagccgaaag cgcgcggtca cccgaaaggt tccagttgtt gtagatattg
     9061 tttttgtaag ccatcgcatc ggtatgcccg gtaataataa ttttgttatc gagcgagtcg
     9121 aaaactggcg cgagctccac cagcagcgtt ttaaagaacg gcataatctg cgcgctgccg
     9181 cgttcgaaca tattgcgatt ctggtcgtct ttaatcagta cgcgtaatcc ctggggaaca
     9241 atctccatct ccagattcgc ttccatatgc gcatcacggg caatggtgtt gatactggtt
     9301 gccagctcgc caagctcggt ggcggatttc ttcttcagca gcgccgtttt ctcattcaca
     9361 tcacgcgctt ttttctccgt ctcttccggc accgccacgg tggcaggttt tggcggatga
     9421 gacgggctga ttttatttaa cggcgacagc ccgccaccgt taaaaatcga ctgcccgtgc
     9481 agcgcggcga taatgctttc acgctcggat ttactgacgc tgttgacaat ccacagcgtc
     9541 ataaacagcg ccatcatcgc cagggtaaag tcggcgaacg ccaccttcca cgccccgcca
     9601 tgatgcccgg catgattctt tttgatctgc cgccggacga tggtggtttt tgccccacgc
     9661 tcgcggcggt tgcccgtctt tctcatgctt attcttgctc cagcatcgca ttcacccacg
     9721 cgtcgaggtt ggcgaaggtc ggtttggacg ccagatgcag cagcttacgc ccggcatcca
     9781 ccgccagcag cgtcggtttg ccgcctgcct gcgccaccag cacagtgcgc acgcattcca
     9841 gcaaagaatg ttctgcccgc gcctgttgtt ccatggcgtt ggcgagcggg tccatcaggc
     9901 agtagcagat aaagacgcct aaaaacgtcc ccaccagcgc tgccgccact ttcaggccga
     9961 tcagggcaat agagccgtca atcgactgca tggtgatgat gatccccagc actgctgcac
    10021 aaataccaaa tcccggcatc gcttcggcgg tacgttgcag cgagcgcgac ggcgtgagca
    10081 gcgactcttc cgcagtgtcc agttcctgat cgagaatgcc ttccagttca tgggcgtcga
    10141 ttttacccat cgccatcagg cgaaagttat cggcaataaa ggtcaccagc cgcttctggg
    10201 ttaacaccag cgggtatttc tggaaaatgg tgctctcttc cggttgctca atatgttgat
    10261 ccagcatgcg cagaccaccg ttttgcacca tttcgagcaa ctcatacagg cacattaata
    10321 gctgacgctg aaactccggc ccgagctgtt ttttactgat cacaccttta atttgatggg
    10381 caatctcttt cagcacatgt ttcgggttac caataatcat cgccccgaaa ccggcaccca
    10441 aaataatgat catctccgcc ggttgccaga tagccactaa ttgcccgccc gcttcaaaat
    10501 aaccgccaaa tacgcagccg agcaccacca gtaaacctaa tattttttgc atgatttctt
    10561 cactttaatt cggatagaca aaatcacgaa ttttctgcgc gatctgttta ttcagttgac
    10621 aaattcgggc ctcggttagc cctaatacca gcgcaatctc tttgagattc atctcatgct
    10681 ggtaatacat ggagaggatc agttgctctt tttcgcttaa ttgtgaaagc gcctgttgca
    10741 gcatttgctg agtgacaaac tgatcttcca gctcccggcc aacaagcgga atgcctggcc
    10801 cttcgccgtt cagcagttcg tccaggctgg cgagcgtttc cgccccttcc agttgctgat
    10861 attcgagata atcttcatgt gagatccctt ccacggccag ttctgaccac tccggttcgt
    10921 gccccagttt tttgcgcgtt tcgcgaatca gatctttcat ctgatgatat ttctgtcgca
    10981 gctggcgcgg tcgccagtcg agagcgcgca gctcatccag gatcgcgccg cgaatacggt
    11041 gcacggcata gcccgcaaaa ccttcgtccg gcagcccgta gcggcggatg gcattaagca
    11101 gccccattaa cgccgtttgt tccatatcct gacgatcaat aatgcaatta cactgcgggg
    11161 caagttgccg caccacttta cgcaccaacg gtaaataggc ctgcaagtag tggctctcct
    11221 gctgcggggt taataccggc gcggcagcaa attcttctga ctcgaataca tcctgtagca
    11281 tgtttatatc cgcctggtga ttactggaag accacttttt tcaccagcaa atccttatac
    11341 ggtgcgctca ttttgcgacg ctgaagatct ttctttaacg cgtcagaaat gagtttgcgg
    11401 atggcagata ttttcatgcc gcgcaaatct tcaaatttca tttccgtcag gcattcaacc
    11461 gcaatacttt gatacagcgg ctcctgatgt tttatttttt ccgcttcttt gtcactggcg
    11521 accaccatca ctaattccgc cgacaaataa tggtccgcgc cgttgttgtc atgcagcgtg
    11581 acaatggttt ccggcaacgt cacaaacacg cttttgcttt catccagggt ttccaccgtt
    11641 tccgtctgcg cggcggcagt cggttttcct tttccggcat aatgatgcca gacaccccat
    11701 ccagctccgg cccccaccac caacgccaga acggcgctga ccacgcccgc caccacgatt
    11761 ttcttcatat tgcctttaca ctttgatcaa aacggattcg ttctgttgaa attgaggcgc
    11821 atcatcaagc tgtaaggcgg cactcacctc ttcctgctga tggcccgact gttgttgttg
    11881 ctgcgactgc ccttcggagg aaacctgtac gttcacctcc ataaaatttt gcgccgtcag
    11941 atgctggcgg agatcgtcgc taaactgctg taacgcgcgg caaacttcac tttggttcgc
    12001 gccgatgtgc accatcagtt tgccgttgtc gagttgtacg gcgatctcga gcttgccaag
    12061 cgacggtgga tcgagacgga tggtggagat ctgctgttgt ttgttgagct gaaagtggat
    12121 ctgatctttc aaaagcgtcg tcagtttttc gcccagttcg tccatcgaaa gcgtggctgg
    12181 tttggcgaca atctcctgat gatcgctgag cagctccggc gtcttaacct gcaacaccgc
    12241 acgcgcaata accggttcac tgtccggcac tttttccgtt ttacgctcga cctgcgggcg
    12301 cgcggtcaca cgggttagtt cagatttcgg gcgagctggc tgtgttgaga cgcgcggctg
    12361 cgtcggcgta atggcatgta aatcttcact agcgtaagtc gccagtctgg cctgctgttc
    12421 gggcttcgcc tgcggcaact gactgatcag ttcctgcaac tgcggcggca acggcgtgag
    12481 ttcctgctgt tgcgtctggc gttgcggcgc gttttgcacc accgctttcg tcaattgctg
    12541 gataaccggc gatgttgcga cgtttcgcgg ctgcggcgtg tcctgatgcg gcgcggcagg
    12601 ttgcggaagt aacagcgcca ttagcgcctg catcgccacc ggatcgttct cctgcgggcg
    12661 cgtcgcctgt tgctgactgg tttttgcgct atgaacgcgc tccgccaccg ccgcgaccgc
    12721 cattttcggc agggtaaacg cgggcgcgtt ttcaccgctc accggcggta aaatatccgc
    12781 cttcaacgaa gcggtttccg caagcgttcc gagcgtggca agcaatgtcg gattcatgac
    12841 ttctccgaaa gtaaattaac aattgagtag gccagtacgc cttcctgatg ccgctgatgc
    12901 tgttccatac gcgctttcaa cacttctgcg cgctgctcgc gccgtttaat cagggcgtta
    12961 aacgcgtcat acagtcgcgc tttcactgca tccaactgcg ccgcatcggt ttccgccgcc
    13021 gcgtaatcgt gaaactggcg tatcaactgc tgatgcagtg ccggaaggcg tcgccagcgt
    13081 tgcttgtcga gggcttcatt catcgcctcg accagtgcga aaagtttcct gcgttgtttt
    13141 tccatcgtct taacccagct tgcccgaaag cccttcccag ccttcgcgca ggttgcttaa
    13201 gatcagcatc acttcatcga ttttctctgc cgacagttcg ccgctggcct catacaatcg
    13261 gtaaacgcag tggtcataga gtcgcgaaag gttgaccacc agctcgccgc cagtttcaaa
    13321 ctccagcgaa ctggtgagcg cattgagaat gtcgatgcat ttattgatgc tctgggcttt
    13381 cttctcgaaa cgccgccctt caatatggct tttggcgcgt tccagttcgt ccatcaggcc
    13441 agaaaacagc accagcacca gttccagagg cgatgccgcc gcagtacgcg ccgcgagatc
    13501 gatctcttta tattgcgaat agccgtcctg tacttggtac atctttaggg atattccttc
    13561 tgagttacgc gaatgccgcc atgctggctt tcatggtgta cacctcgacc agcgtgttgg
    13621 tgtactcctc gagataacgg tcgtagttgg cgttataggt atcagtaagc tgatcgcctt
    13681 cgtcctgaat tttgctcatc tgatcgtcaa tgttctgctg gcgcagggtg atgatgccgt
    13741 tgctggagtc cgtgtaggtg ttaataaggt cgtccatctg cgccaccatg ctgttgtcgc
    13801 caacgaaaat agaggtcaga ccgtcaggat ttttcgccat ttcgtcgttg aactggtcgg
    13861 agtcaatctg taagtggccg tgagaatcga gggtgatgcc atagtcaaca atcgacacgc
    13921 cgttgtagct ggcatgggcg atgtcatcga gctggttcgc cagtgaactg agtccggcgt
    13981 cgcctgcaaa taccccggcg ctggtactgt catcaccgtg ggtggttagc gaatcaaccg
    14041 tatcaatcaa ggtgttatag gcatcgacaa aggtctgtac tttctcctgg ctggcgctgg
    14101 aatcttcgct gatgttaaag gtagtgagat cgctgtcaga atcgctgact tcggtgaaag
    14161 tcatggtgac accgggaatc acatcatcaa acgtattgct gctgttggtg atctcaggcc
    14221 ccgtggcgct gccaagatga ataatcgcgt cctgagcgga ggaaacatcc gtggcaacgg
    14281 gcgcgctggt gctatcgttg ctggcatcat gccccgttac gctcaccgaa aacgcgctct
    14341 gcgccccggt gctatccgag gtgagcataa tcgttgtggt gccgtcggtt tttaccagcg
    14401 ccgccgacac gcccggatta tcatcggagt cgttaatcgc attcaccagt tcactggcgt
    14461 cgataaaacc gtcgccgtca ccattctgat ctgccgccga cagatcgata tccatggtgc
    14521 tgtcgcccat cgtcagttca aaagtgccgg tggcggaaaa ggcgtcgtcg cccatactga
    14581 acgtggtttg ctgcccctgt gccagttgct caacgaaaaa tgagtagctt cccgcctgcg
    14641 cctgggaatt ggcggaaacg ctcgccgagt cattattgct ggtggcggca aacgtcaccg
    14701 ggccgtcggt atcgctgttc agcgcgtcaa cggcgctctg aaaatcgctc aacgctgaac
    14761 tgagcgaatc caggccgctg ctttcagcat ccagctctgt ttgcttctcc tgcaagtttg
    14821 ccgcctgagt ggcgacatcc gcatacgcga tttcttgcgc aatggttctt gggttgatca
    14881 ttgatgactc cggataacga tgtcaggtta ttggcaaaag gcgtgccaat attttttatg
    14941 tgaataatca atagattaaa aaacaggcgg aaatggcgtt tccggtctgg cggaaaaaag
    15001 cctgccgcag aaagcacaac accgcccgca ggcggtgttg ttcgtagtcc gaataaggcg
    15061 tttacgccgc atccggcaag tccgccatta ctgcagcagg ctggaaacca tgctggacat
    15121 gctgttggac tgtttcagca tggtgatgcc agtctgcatc agcacctgct gtttggtcat
    15181 attggacgct tccgtcgcgt agtcggtatc cataatgtta ccgatagcga cttcggtgtt
    15241 gtcctgcata ctggtcaggt tgttggcggt gtcgttcaga cggttgatag acgcacccag
    15301 cttggactgg atctgcgaaa cgtcatccat cgcggtagag atgctggtga tcatctggtt
    15361 agccgagcca gacgcggtca gctcagtacc gccagaaaca cctgcagtac ccgtggtgtc
    15421 tgcctgatcc gcgctaaagg agttgctgat agcagaaaga tcggtcacca gcgtgttcag
    15481 ttggctggag atgttcacgg tcatggtgtc ggaagattcc gcgcccacct ggaaagttac
    15541 cgcgctctgg aacagaccgt cagtaccggt gttgctggta ccggaaacac cgaacaggtt
    15601 ggtgccgccg taagtggtgt tttgcagcat gtcagacatc tgctgaccca gttcgtcgta
    15661 ttcgtcctgc atcgcctgca gatcgtcgtc gctgtaggtg ccgttcgctg cctgagttga
    15721 caggtctttc atacgaccca gcacatcaga catttcatcg aacatactat cggcggtttg
    15781 cagcatcgcg gttgcgtcat tgatgttgct cagcgccacg cccataccgc tggactgtgc
    15841 tgtcagacgg ttagcaattt gtttgcctgc ggcgtcatcg gcagaagagt taatccggtt
    15901 acctgtcgcc aggcgttcca tagatgtaga caaagaagag ctgcttttgc tgattgcatt
    15961 aacggcggcc attgaggcgt tattggtgtt aatagataac atgatactgc tccttgggat
    16021 taatcgtttt cgtttcgata ccctgtaaaa acgacaccta aggagcaaac ttaaaaccgc
    16081 cgttaaaaaa atttcactgc gatttatctg atgccacttt tagctaaaaa aagcagagtt
    16141 taaattcaga taaaaagatt attagcaata tctgccacat ggccaggtat caaaatatgt
    16201 ctttatcaaa cgcttgatat agttaagatt accatcaatt aacgcgtttt cttctcaatg
    16261 tactgatgcc aaaatttaca ttgcgttgca aatagtacac atttggctat tgtaccagtc
    16321 ctgtcatttc gttacatttc catgccatat agtgattaag aataatccta ttaccataaa
    16381 atattagtta tcacgcagaa aaatcatctt ctcagggacg tataagactg tgctttatgc
    16441 aaagcactat caggaaacat ttatcccttg ctaaggtaaa ctcaactatg agtattatta
    16501 tcaacaactg gcggatggac ccgtcgctta atgccttaat acattgcgaa acgggtgaaa
    16561 cacgtcgtct cggcgaatac cattttattt tgctggaaaa attagcaaaa aatgccgatg
    16621 tggttttatc gcgatcttat ttatgtgccg aagtatggaa gaaccgcatt gtcggcggaa
    16681 acagcctgcc gacggcgatc catgcgttgc gcgttgctat cgatgatgat ggcaaacaac
    16741 agaacattat taaaacaatt ccgaagaaag gttacttatg caataaagag tatgtgtctt
    16801 tgccggactc atcgcctgca aaaaaactga ttattaccga ccagatacag gaaacagtac
    16861 cagaagagat atcttctaca acaccgcctg tgcctgttag aaaaaaacat aaagtaataa
    16921 tggggctagc gttagccgcg gctgttatat ttattggctc gaccgtgggg tattcacact
    16981 tgaaaagtac acctgatgcc ccgcaactgg taaaagagtc aattaacagt ccaagaataa
    17041 aaatatttca ccttagttct ggaaaagaaa ataattccgt accgttactg tcacaaaccc
    17101 tcgcaccagg aaaagacaaa ctcgataatt tattgtcagc gcataatatg acaatgacaa
    17161 cctattataa atatgttcgt aaccgactgg aaagtgatat tgttctgcgc aaccagtgca
    17221 atggtagctg gcaattaact tttaatgtgg aaggctggca gaacagcgat attaatagcg
    17281 cgatgtatca gaatttagag aagttgttaa atactttgca gaaatgctga tgagtgccaa
    17341 tatatgccgg atgcgacgct aaacgcgtct tatccggctg ataattaccc caataaattc
    17401 ctcaccgtct ggctggaaat tcgcgcctga ccattcaccg cctgcaataa aacctgatag
    17461 ttatgattcg cgctatccag cacgccgccg aggttttccg acgcctgcac cgcgttttcc
    17521 gtttgcggga agcgcgacat gccgtcgatc aactgccgcg ccttttcctg ctgtaccgcc
    17581 atctgcgcac gctgctgatt aatgctttct aacgtctgag aaattcccgc accgccctgt
    17641 tgcaggcttt gagctaaacg gtctgcctca gacggctcgg caaaagtttt cagcggcata
    17701 aacgccgact tatcgccatc ggtgcgcacg cttaaggtac gttcgatatg gggccactgt
    17761 ttttcggtgg tggagaaact gatgccgtcc gcttgctgat gtagctgcac gcccacgcgg
    17821 cgcagggcgt tggtcagccg cgtctgatac tgcgcggcgc tgtcatcctc agacagcatc
    17881 accgccgaaa gctgagtctg ccgcccgtca gagacgctga acatgcgcgt tcccggcgtg
    17941 ggattgtgta ccagattcgc cagatccggc gagtgaaagg tcacccgcgc ttcgccctgt
    18001 aacaccggct gcaactggcg gtccaccgca ccgccggaaa gcgcggtgcg tttgtccagc
    18061 atctgcatca gcgccgtatt ctgcgcctgc ccgcctttac gctggctgtg acgatagtcg
    18121 agcaactgct gctccagttg cccgagataa tgatccgcct gctgcaacgt ggtgagctgg
    18181 tcgttaagct gcacgctgta acgctgcggg cgagtggtaa tcagcggcga ggccggatat
    18241 tcactcgccg tcgccgggag tttttgccgc accgcctgag tgggcgcaac ggttgacgac
    18301 gataccgcag cgctatgcgc gccgccggtg gcgagtgatg aagatgtaag tccgacctgc
    18361 atactgttcc tgaaagagtt agagaacgct gaacaaattc aactcactca ctttcgagta
    18421 agtcttgagg gtaacttcca ttgccacttc cagtccggta aaggtgatcg acgccgggcc
    18481 gtaatcgaga tcctgtaatg aaccaatcag ctcatcatta gaggtggaga tatcggtctg
    18541 cgcatcgctc agcatcgaca tggtattttg cgtctcgccg agtgacgcaa tcgaggcgtt
    18601 gaggtcgtcc gaggcggtgt ccaccacatc aacggcattc tgaatatcgc tctgcacctg
    18661 cggatctgcc gggtcgacgt ccgggttttg caactcctgg gatagggaat tcagggtgtt
    18721 gagcacgtct aaattgctgc caaagaaatc actcgccgcc acgttggtgt ccacttccac
    18781 gccgttcgac accgtggtct ggcggtggtc gctgttaccc tgatagctgt agctgtcggt
    18841 gacgccatcg ccgtcatcgt ccactgccac aatcggcggc tgatcgttga tggtgccgcc
    18901 aaacacgtag tgcccgtctt cgtcctgata attgagcgcc gccaccattg attcggtgag
    18961 cgactcaata tcctgcccga ggcttgcgag tgaatcggcg gtattggtgc cattcgccgc
    19021 ttccagcagg tcgtcgcgca ccgccagcag gctgttgttg acgccatcaa ggatcgactc
    19081 ctgctggctt aagcccgccg acgcggagtc gatattgctc tgatactgct caatcgccga
    19141 ctgctggcgg tttaactggg tgattcgcgt ggcggcgatc gggtcatccg acggctgcaa
    19201 aatctgcttg ccggttgcca tctgctcgac aacatgcgcc agatcgccag agagattatt
    19261 aaaactttgc gtcatcgaga cgtaggtttg ttgggtagtt actcgcattt tcgcgctccc
    19321 gcttagctgc acatttccag caccgaatcg aaaatctcgg ccccggcgga aatgactttc
    19381 agattggctt cataaatttg ttgataggtg atcaggttca ccgcctcttc gtccatgctg
    19441 acgccgctga cgctgctctg ctggttttgc gctgcggaat agacattgga cgcggcatcg
    19501 acttccgtct ggttttgctg actgtaaatg ccgatattgc tgatgattga cgagcacgcc
    19561 tgcccgaccg tcacgctgcc gaggttggct atttccagcg gttcggtgga gatattgatc
    19621 agcgcctgca ggttgtcgct gttgccgctt tcgtccggtg aactggaaaa cgccagctca
    19681 tcggcggtga tattcgggtt aacggtcagc gggccatcgg cattgctggc gtcataaata
    19741 aacagcggct cgccggggtt accgttgaga tcgtagccct gcgccagctg gttgttgacc
    19801 gcatcggcga actgcgacgc catgctgttg atggtgtcgg tcagcggcgt cagcacatcg
    19861 ttttgataat caaacagtgc gcctaatgaa ccgccggtgt cggtggtcat cgtcgaggtg
    19921 gtgccagcga aggtcagcga catggtcgac gtgccatcgg cgttggtttc cagcgcgatg
    19981 gtcgaacttt gctgcccact caccagcggc tgaccgtttt tcagggtaac gttgtagttg
    20041 ccctggtcgt cgatattgac ctgcacatcc atcatcccgc tcagttcttc caccatctgg
    20101 tcgcgggcgt cgtacagcgc ggaggcgtta tcgccattgg cttcagcctg agcgatttgc
    20161 tggttatagc tggcgatgcc gctggtgagc gtattgattt gcgacaccat cgcctgctgc
    20221 tggctgatga tttccgtgct ttgcgagtcg atgtaatcca gcgtgttatc aatacgcaac
    20281 gacagcgccc cggcttcact gatcacctgc tcgcgcaggg cggaatcatc ggggctggtg
    20341 gttgcttcgt taagggcggc gaagaagtta tcgaagccgc cgctcaggct gctgttatcg
    20401 tcgctcagta ccgcttccag ttggctgaga tccccctgct gggtgctgta atagccgtaa
    20461 tcgctggcgg cataccacac ctgattcacc tgatactggt tggagacgcg gcgaatgctg
    20521 tcgacctgta cgccgttacc ggcgctgttc ggcgaaccgc cgctggcacc gatggtgctg
    20581 atctccgcca cctgacgggt gtagcccgtg gtcatggcgt tggcggtgtt ttgcgccgtg
    20641 acgttaagct ccacctgcgc ggttgatgca ccgctgtagc cgatgttaat catgtccatg
    20701 ttggatcctt accggtaaat cgtgccggtt aaaagcggcg taaagttggg gattcttaag
    20761 ttccgtaaac tgggtgccga acgccggatg cggcgtggac gccttatccg gcctacatac
    20821 gggcgactcg taggcctgat aagacgcgac agcgtcgcat cgtgcaccga acgccgaagc
    20881 tcatttatgc ttcggcgaca actgcttcac aatcatcgcc gcaatgccaa tcccgtgctg
    20941 ctgcgccagg gtgttacaca actcatcgtc ataaaacccc tgcatcatgc gcaccgaatc
    21001 gctgttaaac gggttatctt ttgaatcaaa caactcgttg gttttccgca tctctttcag
    21061 catgttgcgt aaaaaaatcg cctcaaactg ttcggcggcc tgcttgatat cattggcctg
    21121 aaccttcggc cccattaacg cgtcgctgcc gtcaatcccg ccgctggcgt tcactttcat
    21181 cagatcacct ccaggtcggc atccagcgcg cccgcctcat gcagcgcctg caaaatcgac
    21241 ataatgtcgt ccggcgtcgc gcctaaactg ttcagcgcat tgacgagcgt tttcaggctg
    21301 ctcgactccg gcaaactcac cacgcccgga cgggcatgat tcaccgcgat attgctctgc
    21361 ggcgtaaccg ccgtgcgccc acccgcaaag gcattcggct ggctgacgtt gctggtttcg
    21421 ttaatcgaaa cggtcaggct gccgtgcgac accgccgccg cgtgtagcgc aacgccatcg
    21481 cccattacca ccgtgccggt acgcgagtta aacaccacgc gagcgcggat tttctccgcc
    21541 tgcacctgca catcgtccag ttgcgacata aatgccaccc gcgcgccggg gctggtgggc
    21601 gcgcgcacgg taacgttggt ggaactctgc gcggtggcaa taccgccaaa cgaggcgttg
    21661 atcgccgccg caatgttgtt ggcgtcttta aacgacgggc gtttcaggtt gagggtgatg
    21721 gtatcgccca tctgaaaatc gctggggatc tcgcgctcca ccgttgcgcc gttaggaatt
    21781 aacccggcgg ttggcgtatt aaccgttacg ctggaaccgc tggccccggt ggcgttcatc
    21841 ccgcccacca ccacgctgcc ctgtgccagg gcgtaaactt ccccatcagc gccgtgcagt
    21901 tgggtcagca gcaaagtgcc gccgcgaata cttttggcat cgccaatcga cgaaacggtg
    21961 acatcaatgg tctgcccgcg cgagtacatc ggcggcagcg tggcgctcac cgccaccgcg
    22021 gcgacgtttt tcaccttcgg gtcgatttta ttcggcagtt gcacgccaaa ctggcgcagc
    22081 atattggtga tggtctggtt ggtgaatttg acctggtttt tatcaccggt gccgtcgagg
    22141 ccgaccacca ggctatagcc caccagttgg ttttcgcgca cgccctgtac gttgaccagc
    22201 gactccagcg attgcgccag cgccacacca ggcagcgcca ggctcacggc gataactgcg
    22261 gcctttatcc agttttgcat tgcactctcc ggcattaaat cgggaacaga ggatggttaa
    22321 acagccgcgt cagccagcct gcggcgttgg cgtcgcttaa cgcgccgcgt ccggcatagg
    22381 agatgcaggc atcggcgatc cgctgggagg aaaccgagtt gtcgttctgg atgtcgtcgg
    22441 cgcgcaccag gccgcttaag cggatgtact catcgccctg gttgagggtg agccatttct
    22501 cgccgcgaat ttccaggatg ccgttgggct gcaccgcatg gacggaaacg gtgatctccc
    22561 cgcgcaggct gttctgctgc tgggaagtgg cgctgccgtc aaagtcgcgg ttggcgtcga
    22621 tagagccgga aagcttatct ttagtgtgac taaaaatggt cggtgcgccg atatcgacgg
    22681 tgttgctttt gccgaagttg gttttcgcct gtttgctcga ctgggtggat tcttcgagga
    22741 tcaccgtcag gatatcgccc acgcgataag cccgccgatc ggcggtcagc gaccagttgt
    22801 agccagtttc aaatacaccg ccagcacgcc cgtctgccgg aggcggtgct tcggttttcg
    22861 gtggcgcaaa gtatgcgtcg tcttttttga ccagaatggc ctgcgattca cacccgctaa
    22921 gcaggggaag cagcgccacc agccagagat aatttttcac gttctgcctg tttaaataca
    22981 gtgagttatg cgcttttgta ggtcggataa gacgcgtcag cgtcgcatcc gacatttcgg
    23041 ccttacagcg tctgactgat gtactgcaac atatcgtccg cagccgacac cattttggcg
    23101 ttcatctcgt aagcccgttg cactgtaatc atcgccacca tttcgttgac gatatctacg
    23161 ttcgagcctt cgagggcatt gtcctgcaaa gtgccaagcc catcttcgcc cggcacacct
    23221 tcggtcggct gaccgctggc ggcggtttcc agatacaggt tgtcaccctc agcggaaagc
    23281 ccagccgggt tagcgaagtt aaccagcgtc agttgcccta actcggtcac gtcgctgtcg
    23341 ccaggaagaa tcgccgtcac cgtgccgtcc tcaccaaacg caacattcgt cgcccccgcc
    23401 ggaacgtcga tttccggctg caatggcaag ccctgtgagt tggtcagtac gccatcggcg
    23461 ttcacctgca aattgccgtc gcgggtatag gcgatatcgc cgttggcctt ttgcacctgc
    23521 aaaaatccct gccccataat ggcgacattc atcgcgttgt cggtggtttc gacgttgcct
    23581 tcggtgaagg ttttctgggt gccgacaatg cgcacgccgc taccgaattg cagcccggtc
    23641 ggcatgatgt tgttctggtc gagcatcgcg cccggcgctt cctgggtctg gtagaacaga
    23701 tcctgaaaca tcacccggtc acgcttgaag ccggtggtgt taacgttggc gatgttgttg
    23761 gcaatcgcgc tcatttcagc atcctgcgcc gagaggccgg ttttgctgat ccataatgct
    23821 gcgttcatgg tggtaaattc cgtgttattt ttgggccgcg gttgccggat gcggcgtgaa
    23881 cgccttatcc ggcctacgat gtgcggttac gagccgcgca ataagcggtt gcccgcgtcg
    23941 ctgatatctt cggcggtttt catcatcttg atctgtgctt cgaactggcg gttcatggcg
    24001 atggacgaca tcatttcgct taccgccgag acgttgctgc cctcgagaaa accgccgctg
    24061 actttgatgt tttcatcgcg ctgggccgga acgccgtcgg cagtcaccag catcccttca
    24121 gcatttttct ccaggttagc caccgggata tccaccagct tcaggcgatc gatatccatc
    24181 gtcgcggtca catcgccgtc atcgggcgtc accgacagcg tgccgtcgct gccaaacgaa
    24241 gcaatggcgt tcggcggcag aatgatcggc ccgttatccc ccagcaccag attgccgtcg
    24301 atggtcagtt gcccctggtc gtcctgctga atgttgccgt tgcgggtata gacttcgtta
    24361 ccgtttttat cctgcaccgc aatgtaaccc gcgccctgaa tcgccacgtc cagcgggcgt
    24421 tcggttttct ccgcaacacc cgtgctgtcg ttcacgccgc tggccccttc ctgcgccata
    24481 tagcgcgtgt cgaagccgcc gcctttcacc ttgtcgttgg ttgccattgc catatcggcg
    24541 cgaaaaccat tggtgttgac gttcgccagg ttgttggcgc taatttgctg ctcatacagg
    24601 gtctgagaag caccgctcag ggcggtataa atcaggcggt ccattacatt gcctggaaca
    24661 gcgcgtcatc gagctgcgtg ctggtggcga taactttggt gttggcctga tagttacgct
    24721 gggcggtcat caggttgacc agttcgctgg tgatatcgac gttagagctt tcgagcacgc
    24781 ccgacgacag cgtaccgcag gtgccggagc cgggaacgcc aatcagcggg gtgccggatt
    24841 cgcccgtttg tacccatgcg gtgccgctca ctgcctccag gccgttttca ttcgggaaag
    24901 tcgccagcac cacctgcccc tgcaacatgc gctcgccgtt gctgtaggtg gcgtaaactt
    24961 tgccatcgtc atcaacctga acaccgtttt gcgtggcgga ggcgtagccg ttggcggcgt
    25021 tggtggtgac cgaaaattca gagccgtatt gcgtacaggt ggagtaatcg acggttaagt
    25081 cgacgggcgc ggcggcgtcg gtttgaaatt caatggtctg cggcgtggtt ggagaggtca
    25141 gcttcccggt gttcggatcg aaggttaacg tcgtcgccgg aacgccggtc tgctgctgac
    25201 cgtcgaaggt gtactgcact tcccaggtgt tgtcgctggt tttggtgaag tactggcata
    25261 ccgaatgttc gttgcctaat gagtcataca ccgtggtggt gtagctgtcg gtgtacgaac
    25321 tgctgttagt cgcgtcgaac ggaacggtgg tgcgatcgat agcttcatcg ctggcgttga
    25381 agttagcggt aaaggtcagg ctgctgctgg cctgcgccgg gatattgccg gttttgatct
    25441 ggatatcggt gaccgtaccg gtttgcagag tgccagtgtc atcgaccgga tagccctgca
    25501 aataagcgcc cgaggcgttg acgatatagc cgtttttgtc ggtttcaaac gatccggcgc
    25561 gggtatagga gatattgcct gcgctgtcac acatgacaaa aaatccgtcg tcgttaatcg
    25621 ctaaatcgag cgcattgccg gtggagacca gcgaaccgcc acgcgaaata ctttgcgcgg
    25681 tgcccgccac gctgacgccc atcgcctggc ttccggcata catggcggaa aactgggtgg
    25741 tcatcgactt atagcccacc gttccggcgt tagcgatgtt gttactgatc ccgtccagct
    25801 gttcgttaac ggcattcagc cccgttgcgg caatttcata actcattgtg tcttcctgtt
    25861 aattgattct tacgccacat cactgtcggc atcgggtacg ccaaactggc taatcatggt
    25921 gaacggcact tcgcccacgc ctgcaacgtt aagcaccggg gaactgccgt cgagcgggat
    25981 gcgcacatct tccacttcgc cggagacttc aatcggcacc tcttcttccc cggtattggt
    26041 caccaccgag acgtcgtaat cgcccggcgg gatgccgtaa tccgcgggat tcaccgagaa
    26101 atcaacctgg cctggtccga cggacgaact gccttctggg atcagcgaga cggtgtagtc
    26161 atcgcctgcg gcatcggtaa agtggagatc ggcggtggta caggcgtcgt ccagcgtagc
    26221 gcgaccgtta atggtctggt cgctcacctg caaggcggtg gtctgcacca taatgtcgtc
    26281 gcccaccagg ttaccgagcg ccatcacctg aatgttgctc atcagcaccg cgttggtgtt
    26341 ggcctgcacg ctcatctctt ccatcatcgc cacctgcgcc atcgacgaca gctggtcgat
    26401 gtattcggta gcgtcggtcg ggtcctggtt ttggatctcg gcgacaaaca gcgtcaggaa
    26461 ggtatcgaca ggcgagctat cgctgctgct ttgcgtgtcg ctggccgcca cattattgct
    26521 ttccgccgtg gcggtcggct gcggcgattg cgcattcagc gccagggtat tcatcaggct
    26581 tctcccagtt tcagcaaact ttgctgcata cttttcacgt tgttgagcac atcgacgttg
    26641 gtttcgaaat cgcgcgaggc cgacatcatg tccgccatct gctccaccac gttgacgtcg
    26701 ggataccaga cgtcaccgtt ggcgtcggcg agcggagaat gcggttcata gcgtttcacc
    26761 gccccgccgg tttgcagcac gtcctgtacc tgcacgctgg caccgtcaat ggcatgacga
    26821 tgtgtacccg ccagcaggct gtggtgatac accgcggcaa acaccgggct gcgcgcttta
    26881 tacgcctgcg cctcgctgct ggcgggcgca ttggcattcg ccaggttact ggcaacggta
    26941 ttcagacgta gcgtttgcgc cgtcatcgcc gagccggaaa tctgatagat atcagtaaat
    27001 gacatgctta tttcccttca atggcctctc tgatgccgct gatttgcaga ttcagaaagg
    27061 tcaaactgct ttgataatcc atactatttt gtgaaaaccg cgcctgctcg ctgttcagtt
    27121 ccacggtgtt gccatcttcc gacggctgca tcggcacgcg gtactggaca tccaccgccg
    27181 cgttatttgc ccgttgcatc tccctggcaa aatcaatatc tttagccttg aaatttggcg
    27241 tatcgacgtt cgccagattc gcagtcagta gctcggtcct ctcaagacgt aacttcactg
    27301 cctgcggatg tactcccaat gcctgctgaa aactgatccc catttcctgc atcactcctg
    27361 cttcaatccg catacgctaa gcaatcgcca tgccattttt taagtcactg atgagaaagg
    27421 gtttatgagt tttcaatttt gcaggcggaa gttttacttc ctcctggcgc tggccttacc
    27481 gggctacgcc gccgtccatc ccgtgcaaca cagcgcgcgg gaacaagtca acgcccaggt
    27541 gctgaatgcc gcgagccaaa aaattgaatc gctggcgcag cagcggcagt ggcatgacta
    27601 tcgctacacc tttaagatct acattccctc gcagattgcg accgccgcgc cctgtactaa
    27661 aacacccggc gtcacgctga cttcgcctgc ggaaatcgcc ctcaaccgga tgaacttcac
    27721 cgttagttgc ccgcaaagct ggcagatgaa cgtggcggtg cgcccggatg tactggtgcc
    27781 ggtggtaatg acgaagtcac tggttgcccg cgatatgccg ctcaccgcga atgatgtgga
    27841 gcttaagccc tacaacgtca gcgcccagcg ccgcgatgtg ttgatggagc ttgatgacgc
    27901 gattggttta agcagtaagc acgcattgca accaggcaga cccatcacca aagaagaact
    27961 ggtgtctccg gtgctggtcg ggcgcgatca gccggtgatg attgtttacc agagcgcggg
    28021 gatcaccgcc tcgatgcccg gcgtggcgct gaaaaatggt cgtaagggcg agatggtgaa
    28081 agtgcgcaat gccagcagcc agcgggtcat cagcgcgatg gtggcggaaa gcggcgtggt
    28141 gacaacggta agcgcggagt aatttgcgca ggcgattaaa gttttgccgc ctgttaaccg
    28201 ctttaccgca gtaacccacc caaataccga gacgaggttg cgatgaaaat caccccgact
    28261 atgcctggca atcgcccgac agccacaacg gcaggctcca gccagccgcg caaaaccacg
    28321 gtgaccacca ccacgacgct gagcgccgac gacattactc aggcgggatt gcagtctgcc
    28381 cagcaaacgc tgaacgacca gcaagagagc gatatcgaca gcaacaaagt ggcgcaagtg
    28441 caggcgatgc tggcgtccgg cagcccgcaa gtggacactg aacagttagc cagcgacatg
    28501 ctgagttttt tccagaacta agaggccgat gaagtgagta tccggttaca acaggtgaag
    28561 gcgttgttgc agggcattcg tgacgatgac gcgctgtatg attccctgcg cgaacttctc
    28621 cagcgtcagc ggatctgcat gattcgccgc gccagcgaag agttgctggc ggtaaacgat
    28681 gagataaccc atcactatga gcagttgcat ggacatagcc atcagcggca tagcctgctg
    28741 aaaatgctcg gtgttagcgt aaatcgtgac ggtctggcgc aggttttcac ctggcttccg
    28801 gctgttcaaa aagcggctgc acaacagttg tggcaacgcc ttgagcaaaa agcggaacgc
    28861 tgcaaagcct acaacgacaa aaacggcgag ctgttgattc gccagtatga atttattcag
    28921 tcgtttttag gcagcgaagc agattttctc tatcaggaat aacgcgaata ccttccccat
    28981 agcggaaatg gattttccgc ctgattacca atcccttgtt tttaattaca aaaatccccg
    29041 gcacgcttat tgcgtatcct gcacagcatt aaagactgga agccacctat gagttttatt
    29101 gaatgttacg aaccggaata cgtgcgcaaa tttttagcgc gtcaccccgg ttcgccttta
    29161 tacgtccgca aggtgtggaa aaacgagatt cgcctgagcc tgtcattgac cgatattgcc
    29221 agctgcgaag cggtgctcaa cgatgtcagg gcctttgatt tacagctcac ctaccgccct
    29281 gtggaagata attccgcaga gctttccgcg cgcgatgcga tagtaaacca ggtgattttg
    29341 agtaccctca cgctccgtga tttaaccccg gagctttccc tgtatgctgt cgggattttg
    29401 ctctcctgcg ccagcaaaat gccgggcaga gacggagaca tcctggcccg tttgacgacg
    29461 ctgccacagg cgctggccga tcatgcgaaa aaaggtacgc tacaggcaca gtacgcccag
    29521 ctcccgcccg tgccacagct ggcgcgccag ttgatgacgc tgctcggcgg ttgtgcgttt
    29581 gactggtcga ttttgccgga aagcccgcgc aaagcctcgc tgccgttgca ggttacttta
    29641 ctgacgctgc atgacgccaa cagcgaagcc ctgttacaac agcagcttca gacgcagtgg
    29701 caaaccacct ggcaacagca ttttgccacc gcgccctgga tgatgcgtaa ctggctgatt
    29761 taccgcgttt accacgatat gattgggcag actgacgggg cggattattt cccgctggtg
    29821 tgcgattttt atctcctccg cacgttaatc agtttatgga cgctggatgg ttctccgctg
    29881 cggcaggagg atattttcgc cctctttgcc atgtttgaac gctggcgagc cagtgaaaat
    29941 gcgctgttgg ttcgccagca aattcagtcg ttatgtgccg ccgacccgct gctttcagcg
    30001 ttttcgctgc taacgtgagt cgctctgcgc gcgctttgcc cctcacccta accctctccc
    30061 caaaggggcg aggggaccga tcgagcacgg tgttaaaatc ggcgctgatt ttctccctct
    30121 ccctgtggga gagggtcggg gtgagggcat cagcgcgcac gttcatcaca ttcacacatt
    30181 aactcaccca aacaccatgc caacaccaac catcatcaaa acactcgcca cccaccgcga
    30241 aaaaccgttt gtcagcgtcg tcacgccaac ctggaaccgc ggcgcattcc tgccgtacct
    30301 gctctatatg taccgctatc aggattaccc ggcagaccgc cgcgaactca tcatcctcga
    30361 cgattcccca caaagccatc aacacatcat cgaccgcctg accaatggca cgccggaagc
    30421 ctttaacatc cgctacatcc atcacccgga aaaactgccg ctgggcaaaa aacgcaacat
    30481 gctcaacgaa ctggcgcgcg gcgaatacat cctctgcatg gatgatgacg actactatcc
    30541 ggcagataaa atctcttaca ccatcgctat gatgcaaaaa caccgggcgc tcatctccgg
    30601 ttccgaccag atccccatct ggtacagcca tatcaaccgt atctttcaaa gccgcagctt
    30661 tggcccacat aatatcctca acggcacctt ttgctatcac cgtaactacc tgaaaaagca
    30721 ccgttacgac gatgattgca atttaggcga agagaaaagt ttcaccgaca atttcagcgt
    30781 taacccgctg caactgcccg gcgagcgcac gattttgtgt atctcgcata gccacaacac
    30841 ctttgataaa gactttatta tcggggccag cacgccggta aacacggcgc ttacggatat
    30901 cgtccgcgat ccgctgctgc gcaatgctta tctgagtctg cataacgcca ctcatcacca
    30961 ggcgatcaac catcaggcga tcgatcaggt ggtcgtcgtg aatctggata aacgcccgga
    31021 tcgcttacaa cagatccgtg aagaactggc attgctgcat attccacccg agaagatcac
    31081 ccgccttgcc gccagtgaag atgaaaacgg tcaacggggc cgccgtcagt cgcacttgca
    31141 ggcgctgcgt ctggcgcaac agcacggctg gcaaaactat ctgttactgg aagatgacgc
    31201 ggttattctc aagcaggaga aacatattca ggtactgaac acgctgctgg cgagcctggc
    31261 gaaaattccc tggcaggtga tgatcctcgg cggcgaaatt tcgcaaggaa cgatgctaaa
    31321 aagtttgcct gggctggtgc acgcgcggga ctgccgcaaa gtgtgcgctt atctggtgaa
    31381 cagccgctac tacccgcaac tggcgcagca gatgagcaac gatgagcact cgctggaaga
    31441 ctgctggcaa ccgctgctac gcgccgacaa atggctggcc tgttacccga gcctttgtta
    31501 tcagcgaccg ggctttagcg atatccagaa aaaaacgact gataatattg ctcgctattt
    31561 caataagtta cccgtaacca ccaagccatc cgcgttaccc atcgccgaca ccatcggctt
    31621 ttttatggaa acctcattcc actacgcgct gtaccgccca atcatcaccg ccctacaggc
    31681 gcaagggctg agctgcacgc tggtgatcaa cgaccgcgtg tttaaaccgt ttctggatga
    31741 gatgctggaa acactgaaaa acatcgacga tccgcagctt aaaggtatgc gtctgtcaga
    31801 aatgcagaca catggtcagc gggtgaaatg cctggtcagc ccctaccaca cgcctgcgct
    31861 caatggcctg gcggcggtga acatccgcgc tatgtacggc ttagccaaag agacgtggaa
    31921 ccacgccgac tggaatcgtt tttaccaaag cattttgtgt tatagccact acagtcagca
    31981 ggcgcttgcg catttcggca gcgcaaaagt ggtcggcaac ccgcgcttcg acgcctggca
    32041 taacggcacg ttcgatcgcg cgttgccgga aaacatccaa tcagattacc gtaaacctac
    32101 ggtgctctat gccccaacgt ttggcgcatt aagctccctg ccccactggg ctgaaaaatt
    32161 gggccgcttg agcggtgatg tgaacctgat ttgcaaactg caccatggca cctgctcacg
    32221 cccggaagaa gcagcatcgc tggccctggt gcgcaggcat ttaaaacagc gcaccgactc
    32281 cgcccgccac acgctggcgc tgctggcaaa agccgattac gtgctgaccg ataacagcgg
    32341 gtttattttc gatgctatcc acgttgataa gcgggtgatc ctgctcgact ttccgggcat
    32401 gaatgatttg ctcgacggtg agaaaagtta ttccacggca gagagcgccg accagcgaat
    32461 ccgcgaaata ttgccggttg ctcatgatgt tgcagaactg cgttatttgt tgtcagaggc
    32521 gtttgactgg gggtcagtgc aggcgcggct tacggagatt cgccatcact attgcgatgc
    32581 gtttatggat ggcaaggcgg gagaacgggc ggcgattgtg attgtggagg cgctggggta
    32641 aaccgcgcaa ctgcctgatg cgcttctctt atcaggccta cggatcgatc acgtcctgta
    32701 ggccggataa ggcgtttacg ccgcatccgg caagaaaata catctctgtt ttagtacttt
    32761 ataaaagccc tctaatcacc tcaataatcc ccgtcgtcga caccgacggc gtgcggggga
    32821 aatagaccac ctcacaaata tacgcaaagc tgtcaaactt ccctgcccag tcatccccca
    32881 tcactaaaat atccgcactg tacccgcgca aatactccgc tttctgctct agcgattctt
    32941 ccagaaatac gccatccacg cacgccagcc cggcgatgat cgccatgcgg tcatcctgat
    33001 ggtaaaccgg tgccctgccc tttttggcga tgttcagggc gtcggacgat acgccgacca
    33061 gcagccgctc gcccaatgcg cgggcgcgtt gtaaaaggcg cagatgaccg acatgaaaca
    33121 catcgaaggt gccgaaggtg atgaccgttt tcatggctac tccgctaatt tgtgccgcag
    33181 ccagcactgc acggcgatgt cgtccaccgc tttttgcgct ttcgccgtgg cgctttcttg
    33241 ttgctcgcgg cgtttgtcgt ccagcgtcag ggcgactact ttctcttcgc acgccgccgc
    33301 cgtgaggttt ttctgcattc gcgtggcttt gatattggcc agcgtttttt cctgctcctg
    33361 ccaggcgatt accttgcgca gggtgccttt gtagccgctg acgtttttca gcgattccac
    33421 cgacaccgcc gcacccgcag atgttttctc gacaaggtag cctaacgcct tgatattgtt
    33481 gtcataaccc gcgcaaatct gtttttgctt tgccagctcg acggttttgt ctttgaccga
    33541 tttatcccgc aggcgctgca actgcgtcag ggtgtcgatg atttgtcgca tagttactgt
    33601 tctgcctttt tgacaccagg aaagagaatt tgcaggcggc tttgcaccag ttcgagggag
    33661 gcgggttcgc gcatctcctg gcgcaaaaat cgctcgatgg ccgggaacat ttttaccgct
    33721 ttgtcgacgc tggcgtcggc acctgccaca taaccgccga gcgggatcag aggtttgatc
    33781 tccatatagg cggcatagtt ttgctttaac gcgcgggcgg attgctgatg ttccaggctg
    33841 gtgacctggt tcatgcagcg gctgatcgac tggccgatat caatggccgg atagtgcccc
    33901 gcttccgcca gtttgcgggt taatacgata tgcccgtcca gcaccgcgcg cgcgcagtcg
    33961 acaatcggat cttgctggtc gtcaccttcc gccagcacgg tgtagatggc ggtcatcgat
    34021 cccgcgcttt cgctgttgcc tgcgctttcg accagtttgg ggatcatgcc gaatgccgat
    34081 ggcggataac ctttggtggc gggcggttcg ccgagcgaga gggcgatttc ccgctgcgcc
    34141 atcgcgtagc gggtcaggga atccaccagc agtaagacgt gatgcccgcg gtcgcgaaac
    34201 caggcggcga tggagtggca cagttcggtg gctttcagac gcatcagcgg cgattcatcg
    34261 gcaggcgcga cgacgacgat agactttgcc agcccgtccg cgcccagcga gtggtcgata
    34321 aactctttca cttcgcgccc gcgttcccca atcagcccaa ccaccacgat atcggctttg
    34381 gtctggcggg tgatcatccc cagcagcacg cttttcccga cgccgctgcc cgccattagt
    34441 ccgacgcgct ggcctttgcc gatggtcagc aggccgttga tcgctttcac gcccacgtcc
    34501 agcggttcgt ctaccgaacg acgagttaag ggattaaccg agggcggcaa cggcggcaga
    34561 aggtcgttgc cgttcaggcg gcctttgcca tccagcggtt cgccgaggcc attcaccacg
    34621 cggcctaacc agctttcgcc tatcaggatg tcgtgggctt tttcttccgg gaagacgcgt
    34681 gcgcctgcca tcaggccgac cggctgttta aacggcatta aataggtgat gtcgcgatta
    34741 aagcccacgg cctgcgcgtc gatcagcgtg tgattggcac tttcgatgcg gcacagctgc
    34801 ccggtcatta gcgggcagcc gacactctcc agcaggatgc cgttaaggcg caccagccgc
    34861 ccggcgacgc gggccagcgg gatcgactcc agcgagcgca gggcgttatc gaacgcagaa
    34921 aaatcactca ccctgcgact ccggcgtgag tgtttctttc agcgcggtca tgcattgttc
    34981 aaggcgatgc tcgcagccga tatccagttc ggatttgtcg gtgatcaccc ggcattcgcc
    35041 cggtggcaaa tccggcgaag gcgtcagccc ccattcgctg accttttccg gcaccgcgtc
    35101 gcgcaggcgg ttaaactctt ccgtgctcag cattacttgc agggtttccg gcatcgccgg
    35161 gaaggccgcc agcgcctctt ccaccagcgc cagcaactgc gtgggttgca gcgccagttc
    35221 acaacggatc acctggcggg tgactttttc caccagttgc agcaaatctt cccgctgttt
    35281 acgttcgata tgagcgagga agtcgttcac tttaccggtg atggcttcca gcggctgcgc
    35341 cgcctcaaca aactgtttgc gcccttcctg ctgcccggcg aggctgcctt cggtgtaacc
    35401 ctggcgtcgc ccctcggcaa aaccttgctg atgaccttcg ctaaagccct gctcctggcc
    35461 ttcggtcatt ccttgagcaa accctttttg cagcccttcc tgaaatccgt ccatcaactg
    35521 gcgctggtaa tcggcggcgc taatactggt agtgagcggt tctccggtca ccgcacgctg
    35581 gcgcggcggg aaacggtgca ggcgatagcg cccgcgcagt gtttcaatgg tcatggttac
    35641 tccgccgtct gttccgcaaa gagctgtagc tgaatttcgc ccgcctcttc cagttcacgg
    35701 gcgatgccca tgatttcgcg gcggatctgt tcgatacggc tgaccggcac cggccccaga
    35761 cgcgcggtga tggcttccag ttgctgcgcc tgccgttttg gcattaccgc gtaaatcgaa
    35821 cggcgcagca gggcttcggt gcctttcagc gccaccgccc agtcttccat cggcacttcg
    35881 tcaagcagac gtcgacgcac ctcttcgttc tggcggctga ggataaagaa gtcgtacatc
    35941 tcatcctgaa gctgctccag cacctcttcg tcgcgctcgc gcatctggtc gaggatcacc
    36001 tgctggttgc cctggaagcg gttgacgata tccgccgcct gtttgatgcc tttcactttt
    36061 gagccgtgtt ccgaaagcac cgacaggccg cgttcgatca gcctgtccag ttcatccacc
    36121 acgtcgcggt tgacgtcgtt gagtttcgcc acccggtaga gaatttcgtt ttgcaccgat
    36181 tcgttgagat acgagagcac cgctgccgag atttccggcg tcagaaacgc gaggaacacc
    36241 gcctgtaatt gcagatgttc ttcggagatc agcatcgcca gctggcgtgg ttccacccat
    36301 tgcaggcgcg ccatccgcga gcggatctca tcgccgtaga tgccgttgat cacactgctg
    36361 gcgatctcgg tgccgagggc tttgttgaga ataccctgca acatggatcg cgatgcgccg
    36421 ttgatcccgc tctgttcgcg gaactcatca aagaagttat tgatcacttt gcgcgccatg
    36481 ctggttttca cgccggaaag ccgtgccatg ttttcgctca ggcgccccac ttcttcgcgg
    36541 ctgagttttt gcatcaccgt cgccgccgcc tcttcgccca gacacagtag caggatcgcc
    36601 gcctgttcca ggtagctgtt gctcttattt gttgtcaatt cgctcattgc tggtgatcca
    36661 ttgtctgaga acttcggcta cgcgatcggt gtcgctcatg gccagttttt gcaggaactc
    36721 gagttttact tccaggccgg aactctgaga cggcaggctg tcatcgccgg gcagcgacgg
    36781 cagatcgacg tttttgcgct cctcttccgg catggcaatc cacggttcgc tgacggcttc
    36841 cgattctggt gtgataatcg gtgcacgtac cgccgtcagc cgtttcatta ccgggcgcac
    36901 cacgaagaac agcaacaaca gcgccagcag gccgcagcca atcaggcgca cccaggccag
    36961 cacgctgtca tctttccaca gcggaataat tggttcgacc ggcaccgcct gcggcacaaa
    37021 gttaagcagt gaaagcgtca ggttatcccc acgttgcgcg tcaataccgg cggcattgtt
    37081 cagcaacgcg gtaagctgcg cggtctgctc cggcttccag tttttcagcg ccggagcgtt
    37141 ttgattgagc accaccgcca cattgaggcg tttcaccgca aagccgggat gctgaatatg
    37201 ctggacgcta cggtcgtaag agtaatcgcg cttgctttcg ctgtgtttgg ataacgcttc
    37261 aggcgagcga ttttcttccg taccgttagt catttgattc gccgcaaccg gcggacggtt
    37321 gctgagagag ccgggcacac ccatcgccac ctgattggtg tcgctgtcca gcacgctctc
    37381 ttcgcggttt attttcggcg cgtcgccgta gtgttcctga gtttcgtcga tggtgctgag
    37441 gtcgagatcc ggcatcacgc tgacacggta attcccgctg ccgaccagcg aatccagcac
    37501 gttcgccacg ctggcgtggg ttttgtcctg aatgtctttg aggatctgat cgcgtttacg
    37561 ggtagcggca gagaccgcct cgcccgcgcc gatgccgtcg gtcagcagat ttcccgcctg
    37621 atcaaccacg ctaactttgc tggcgtgcag ccccggaatg ctgccggaaa ccagatgcac
    37681 aatggcgttc acctggtcca tattcagctt cgcgccgtag tgcagacgca ccaccaccga
    37741 ggcgctgttt tgcggctcat cgctgaccac aaacgagctt tcttcgttga gcgccagatg
    37801 cacccgcgcg ctctccaccg cgtccagcga cataatgctc tgcgccagct ccccttcgag
    37861 gctgcgttta tagcgcacgt tctgcacgaa ctggctgctg ccaagcactt cgtctttgtc
    37921 catcagctcg tagccgctgg gcaaaatcgc ctgcacgcct ttggcggcaa gggtcatgcg
    37981 cgttttcgac agcgcctctt ccggcaccag tatctgcccg ctttgcgggt caatgcggta
    38041 ggtgagcttt tcgccgtcca gcacggtgac aatctgcgac acgggcagat tttcctggcg
    38101 tccgtagagc gagacgtagc cgcgattgcc ggtccacagc acgctaacga taatcgccgt
    38161 cgccgccacg gcaagcccca cacccgccat cagcgcccag cgtttgttat ctgcgagacg
    38221 cagtcgaaac gccggaaacg ccagggttaa cttctttatt tgtgcattca tagggttaga
    38281 tcgacatact catcagatcg ttaaatccgg tggcgatttt gttgcgcacc tgcaccagcg
    38341 cggagaaaga gagcgacgcc tgctggctgg cgatcatcgc acctgccaga tcatcgcttt
    38401 tgcccatgtc gatagccgtt tgctgctgct ccgccacctg ctggaactgg tcaacgtgac
    38461 caagcgcgcc ctgcataatg cggtgaaacg aaacgtccgg gtcggtggaa ctgaaggtca
    38521 tggcgggcag caccggcgcc tgggcgttgg cctgcatccg ctgcacgtcc tgcatgatct
    38581 gcgcctgcat ggtgctgttg attgtggtaa tgctcatttt taacttccgg gtcaggagaa
    38641 aacttcgata ccgtgcttgc gcatggaggc cagtcgatag cgcagtgcgc gcggggtaat
    38701 acctaacagg tcggcgattt tgctgcggtt gccctgatat ttacgcatca ggtcagcgat
    38761 atattgatac tgggcgcttc gtccgtgctg gcctaaatcg ctggtttcgg caatgtgtac
    38821 ggcgggctga gcaggccatt gcagttcgct gtggtcggca atgtcatttt ccggcaggcc
    38881 taaggcgtcc gggtaaatca cgccgtcgcg atttaaaatc attccccgct gaatggcgtt
    38941 ctccagctgg cgcacattgc cgggccagcg gtaattcagc atcgcacggc gggtagattc
    39001 tgaaagggtg atatttttca ccagcaccgt ggagtatttt tttataaatg actctgccag
    39061 cggaataata tcgttcagac gttcacgcag cggcggcatg gtaataggaa taaccgccag
    39121 acgataatag agatcttcac gaaaacgccc ggcggcgact tcctgctcaa ggtttttatt
    39181 ggtgcaggca atcaggcgaa aattgagttt aatttgtcgg ttgctgccta atcgctcaac
    39241 ctgttgttcc tgcaatacgc gtaatatttt ggcctgtaac gccagcggca tatcgccaat
    39301 ttcatcgagt aataaggtgc cgttattcgc cagttccatt tttcctggca cactggcaat
    39361 tgcgccggta aatgcgcctt tgtcgtagcc aaataaggtt gcttccagca tgttttccgg
    39421 gatcgcagcg cagttcacgc cgatataggg cgcgttttca ttttcaccaa aggcgactgt
    39481 gtgaatatat ttcgccacgc actctttgcc cgcgccggtt tcgccctgaa tgagcaccgg
    39541 cacgttaaat gcggcgacac gctttgccag agtaaaagcg ttaatgctgg aagcggcagt
    39601 tgcaataagt tcagacatag ggtgaacgat ataagtagcg acaataatca ggatgttctg
    39661 aagcgtatca ccggcgggat taacaagttc gaattaaacg ataataaaaa cattatttgt
    39721 ttataaacaa tgatttatcc atgtaggccg gataaggcgt ttacgccgca tccggcaatt
    39781 gtgcaccggt gcctgatgcg atgcggcgcg tct
//
LOCUS       DAZFWL020000028        39723 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFWL020000028 DAZFWL020000000
VERSION     DAZFWL020000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.028"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            81..821
                     /gene="yiaT"
                     /locus_tag="ACHKNP_RS07985"
                     /old_locus_tag="pgaptmp_001597"
     CDS             81..821
                     /gene="yiaT"
                     /locus_tag="ACHKNP_RS07985"
                     /old_locus_tag="pgaptmp_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HGU4703791.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITNSPMIDRNESFTFITGVSWRF"
     gene            complement(768..1031)
                     /locus_tag="ACHKNP_RS07990"
                     /old_locus_tag="pgaptmp_001598"
                     /pseudo
     CDS             complement(768..1031)
                     /locus_tag="ACHKNP_RS07990"
                     /old_locus_tag="pgaptmp_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1035..1136
                     /locus_tag="ACHKNP_RS07995"
                     /old_locus_tag="pgaptmp_001599"
                     /pseudo
     CDS             1035..1136
                     /locus_tag="ACHKNP_RS07995"
                     /old_locus_tag="pgaptmp_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(1168..1863)
                     /gene="araD"
                     /locus_tag="ACHKNP_RS08000"
                     /old_locus_tag="pgaptmp_001600"
     CDS             complement(1168..1863)
                     /gene="araD"
                     /locus_tag="ACHKNP_RS08000"
                     /old_locus_tag="pgaptmp_001600"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGU4703792.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(1857..2717)
                     /gene="sgbU"
                     /locus_tag="ACHKNP_RS08005"
                     /old_locus_tag="pgaptmp_001601"
     CDS             complement(1857..2717)
                     /gene="sgbU"
                     /locus_tag="ACHKNP_RS08005"
                     /old_locus_tag="pgaptmp_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HGU4703793.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(2710..3372)
                     /gene="ulaD"
                     /locus_tag="ACHKNP_RS08010"
                     /old_locus_tag="pgaptmp_001602"
     CDS             complement(2710..3372)
                     /gene="ulaD"
                     /locus_tag="ACHKNP_RS08010"
                     /old_locus_tag="pgaptmp_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGU4703794.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(3369..4865)
                     /gene="lyxK"
                     /locus_tag="ACHKNP_RS08015"
                     /old_locus_tag="pgaptmp_001603"
     CDS             complement(3369..4865)
                     /gene="lyxK"
                     /locus_tag="ACHKNP_RS08015"
                     /old_locus_tag="pgaptmp_001603"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HGU4703795.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHTLPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            complement(4869..5855)
                     /gene="yiaO"
                     /locus_tag="ACHKNP_RS08020"
                     /old_locus_tag="pgaptmp_001604"
     CDS             complement(4869..5855)
                     /gene="yiaO"
                     /locus_tag="ACHKNP_RS08020"
                     /old_locus_tag="pgaptmp_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HGU4703796.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            complement(5868..7145)
                     /gene="yiaN"
                     /locus_tag="ACHKNP_RS08025"
                     /old_locus_tag="pgaptmp_001605"
     CDS             complement(5868..7145)
                     /gene="yiaN"
                     /locus_tag="ACHKNP_RS08025"
                     /old_locus_tag="pgaptmp_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HGU4703797.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(7148..7621)
                     /gene="yiaM"
                     /locus_tag="ACHKNP_RS08030"
                     /old_locus_tag="pgaptmp_001606"
     CDS             complement(7148..7621)
                     /gene="yiaM"
                     /locus_tag="ACHKNP_RS08030"
                     /old_locus_tag="pgaptmp_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HGU4703798.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            complement(7739..8206)
                     /gene="yiaL"
                     /locus_tag="ACHKNP_RS08035"
                     /old_locus_tag="pgaptmp_001607"
     CDS             complement(7739..8206)
                     /gene="yiaL"
                     /locus_tag="ACHKNP_RS08035"
                     /old_locus_tag="pgaptmp_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGU4703799.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            complement(8218..9216)
                     /gene="yiaK"
                     /locus_tag="ACHKNP_RS08040"
                     /old_locus_tag="pgaptmp_001608"
     CDS             complement(8218..9216)
                     /gene="yiaK"
                     /locus_tag="ACHKNP_RS08040"
                     /old_locus_tag="pgaptmp_001608"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HGU4703800.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            9417..10265
                     /gene="yiaJ"
                     /locus_tag="ACHKNP_RS08045"
                     /old_locus_tag="pgaptmp_001609"
     CDS             9417..10265
                     /gene="yiaJ"
                     /locus_tag="ACHKNP_RS08045"
                     /old_locus_tag="pgaptmp_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HGU4703801.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            10367..10840
                     /gene="ysaA"
                     /locus_tag="ACHKNP_RS08050"
                     /old_locus_tag="pgaptmp_001610"
     CDS             10367..10840
                     /gene="ysaA"
                     /locus_tag="ACHKNP_RS08050"
                     /old_locus_tag="pgaptmp_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU4703802.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHCWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(10992..12245)
                     /gene="avtA"
                     /locus_tag="ACHKNP_RS08055"
                     /old_locus_tag="pgaptmp_001611"
     CDS             complement(10992..12245)
                     /gene="avtA"
                     /locus_tag="ACHKNP_RS08055"
                     /old_locus_tag="pgaptmp_001611"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HGU4703803.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(12423..14453)
                     /gene="malS"
                     /locus_tag="ACHKNP_RS08060"
                     /old_locus_tag="pgaptmp_001612"
     CDS             complement(12423..14453)
                     /gene="malS"
                     /locus_tag="ACHKNP_RS08060"
                     /old_locus_tag="pgaptmp_001612"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU4703804.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTETFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTQPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDMTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            14773..15597
                     /gene="bax"
                     /locus_tag="ACHKNP_RS08065"
                     /old_locus_tag="pgaptmp_001613"
     CDS             14773..15597
                     /gene="bax"
                     /locus_tag="ACHKNP_RS08065"
                     /old_locus_tag="pgaptmp_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HGU4703805.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSNSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(15705..16883)
                     /gene="xylR"
                     /locus_tag="ACHKNP_RS08070"
                     /old_locus_tag="pgaptmp_001614"
     CDS             complement(15705..16883)
                     /gene="xylR"
                     /locus_tag="ACHKNP_RS08070"
                     /old_locus_tag="pgaptmp_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HGU4703806.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(16961..18142)
                     /gene="xylH"
                     /locus_tag="ACHKNP_RS08075"
                     /old_locus_tag="pgaptmp_001615"
     CDS             complement(16961..18142)
                     /gene="xylH"
                     /locus_tag="ACHKNP_RS08075"
                     /old_locus_tag="pgaptmp_001615"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HGU4703807.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(18120..19661)
                     /gene="xylG"
                     /locus_tag="ACHKNP_RS08080"
                     /old_locus_tag="pgaptmp_001616"
     CDS             complement(18120..19661)
                     /gene="xylG"
                     /locus_tag="ACHKNP_RS08080"
                     /old_locus_tag="pgaptmp_001616"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HGU4703808.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(19739..20731)
                     /gene="xylF"
                     /locus_tag="ACHKNP_RS08085"
                     /old_locus_tag="pgaptmp_001617"
     CDS             complement(19739..20731)
                     /gene="xylF"
                     /locus_tag="ACHKNP_RS08085"
                     /old_locus_tag="pgaptmp_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4703809.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            21097..22419
                     /gene="xylA"
                     /locus_tag="ACHKNP_RS08090"
                     /old_locus_tag="pgaptmp_001618"
     CDS             21097..22419
                     /gene="xylA"
                     /locus_tag="ACHKNP_RS08090"
                     /old_locus_tag="pgaptmp_001618"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HGU4703810.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMAVALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            22491..23945
                     /gene="xylB"
                     /locus_tag="ACHKNP_RS08095"
                     /old_locus_tag="pgaptmp_001619"
     CDS             22491..23945
                     /gene="xylB"
                     /locus_tag="ACHKNP_RS08095"
                     /old_locus_tag="pgaptmp_001619"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HGU4703811.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            24114..24455
                     /gene="yiaB"
                     /locus_tag="ACHKNP_RS08100"
                     /old_locus_tag="pgaptmp_001620"
     CDS             24114..24455
                     /gene="yiaB"
                     /locus_tag="ACHKNP_RS08100"
                     /old_locus_tag="pgaptmp_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HGU4703812.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            24501..24938
                     /gene="yiaA"
                     /locus_tag="ACHKNP_RS08105"
                     /old_locus_tag="pgaptmp_001621"
     CDS             24501..24938
                     /gene="yiaA"
                     /locus_tag="ACHKNP_RS08105"
                     /old_locus_tag="pgaptmp_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HGU4703813.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(24980..25975)
                     /gene="wecH"
                     /locus_tag="ACHKNP_RS08110"
                     /old_locus_tag="pgaptmp_001622"
     CDS             complement(24980..25975)
                     /gene="wecH"
                     /locus_tag="ACHKNP_RS08110"
                     /old_locus_tag="pgaptmp_001622"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HGU4703814.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            26150..26449
                     /gene="ysaB"
                     /locus_tag="ACHKNP_RS08115"
                     /old_locus_tag="pgaptmp_001623"
     CDS             26150..26449
                     /gene="ysaB"
                     /locus_tag="ACHKNP_RS08115"
                     /old_locus_tag="pgaptmp_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HGU4703815.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLSMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            26544..27455
                     /gene="glyQ"
                     /locus_tag="ACHKNP_RS08120"
                     /old_locus_tag="pgaptmp_001624"
     CDS             26544..27455
                     /gene="glyQ"
                     /locus_tag="ACHKNP_RS08120"
                     /old_locus_tag="pgaptmp_001624"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGU4703816.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            27465..29534
                     /gene="glyS"
                     /locus_tag="ACHKNP_RS08125"
                     /old_locus_tag="pgaptmp_001625"
     CDS             27465..29534
                     /gene="glyS"
                     /locus_tag="ACHKNP_RS08125"
                     /old_locus_tag="pgaptmp_001625"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGU4703817.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            29859..30011
                     /gene="hokA"
                     /locus_tag="ACHKNP_RS08130"
                     /old_locus_tag="pgaptmp_001626"
     CDS             29859..30011
                     /gene="hokA"
                     /locus_tag="ACHKNP_RS08130"
                     /old_locus_tag="pgaptmp_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HGU4703818.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(30000..30059)
                     /locus_tag="ACHKNP_RS08135"
                     /old_locus_tag="pgaptmp_001627"
     CDS             complement(30000..30059)
                     /locus_tag="ACHKNP_RS08135"
                     /old_locus_tag="pgaptmp_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703819.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(30199..30411)
                     /gene="cspA"
                     /locus_tag="ACHKNP_RS08140"
                     /old_locus_tag="pgaptmp_001628"
     CDS             complement(30199..30411)
                     /gene="cspA"
                     /locus_tag="ACHKNP_RS08140"
                     /old_locus_tag="pgaptmp_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HGU4703820.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(30692..30982)
                     /gene="yiaG"
                     /locus_tag="ACHKNP_RS08145"
                     /old_locus_tag="pgaptmp_001629"
     CDS             complement(30692..30982)
                     /gene="yiaG"
                     /locus_tag="ACHKNP_RS08145"
                     /old_locus_tag="pgaptmp_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HGU4703821.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            31416..32126
                     /gene="yiaF"
                     /locus_tag="ACHKNP_RS08150"
                     /old_locus_tag="pgaptmp_001630"
     CDS             31416..32126
                     /gene="yiaF"
                     /locus_tag="ACHKNP_RS08150"
                     /old_locus_tag="pgaptmp_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HGU4703822.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(32176..33150)
                     /gene="ghrB"
                     /locus_tag="ACHKNP_RS08155"
                     /old_locus_tag="pgaptmp_001631"
     CDS             complement(32176..33150)
                     /gene="ghrB"
                     /locus_tag="ACHKNP_RS08155"
                     /old_locus_tag="pgaptmp_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HGU4703823.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(33254..33913)
                     /gene="yiaD"
                     /locus_tag="ACHKNP_RS08160"
                     /old_locus_tag="pgaptmp_001632"
     CDS             complement(33254..33913)
                     /gene="yiaD"
                     /locus_tag="ACHKNP_RS08160"
                     /old_locus_tag="pgaptmp_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HGU4703824.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            34066..36399
                     /gene="bisC"
                     /locus_tag="ACHKNP_RS08165"
                     /old_locus_tag="pgaptmp_001633"
     CDS             34066..36399
                     /gene="bisC"
                     /locus_tag="ACHKNP_RS08165"
                     /old_locus_tag="pgaptmp_001633"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HGU4703825.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(36368..36808)
                     /gene="yiaC"
                     /locus_tag="ACHKNP_RS08170"
                     /old_locus_tag="pgaptmp_001634"
     CDS             complement(36368..36808)
                     /gene="yiaC"
                     /locus_tag="ACHKNP_RS08170"
                     /old_locus_tag="pgaptmp_001634"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU4703826.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(36805..37368)
                     /gene="tag"
                     /locus_tag="ACHKNP_RS08175"
                     /old_locus_tag="pgaptmp_001635"
     CDS             complement(36805..37368)
                     /gene="tag"
                     /locus_tag="ACHKNP_RS08175"
                     /old_locus_tag="pgaptmp_001635"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGU4703827.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            37526..38224
                     /gene="yhjY"
                     /locus_tag="ACHKNP_RS08180"
                     /old_locus_tag="pgaptmp_001636"
     CDS             37526..38224
                     /gene="yhjY"
                     /locus_tag="ACHKNP_RS08180"
                     /old_locus_tag="pgaptmp_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU4703828.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            38453..39655
                     /gene="yhjX"
                     /locus_tag="ACHKNP_RS08185"
                     /old_locus_tag="pgaptmp_001637"
     CDS             38453..39655
                     /gene="yhjX"
                     /locus_tag="ACHKNP_RS08185"
                     /old_locus_tag="pgaptmp_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4703829.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         9697 a         9879 c        10208 g         9939 t
ORIGIN      
        1 atatgaatct tgcaccggag aaattgtaag cattattatg ccgactcttt tttatcttat
       61 tttaaaatgg aataccggac atgttaatta atcgcaatat tgtggcgtta tttgcgttgc
      121 cttttatggc aagcgcaact gcttctgaat tatccattgg tgctggtgca gcttataatg
      181 aatcgcctta tcgcggttat aatgaaaata caaaggcaat tccgctgatt agttatgaag
      241 gtgattcttt ttatgttcgt cagaccacat taggttttat tctgtcgcaa agtgaaaaaa
      301 atgaacttag cctgaccgca tcctggatgc cgctggaatt tgaccctgcc gataatgacg
      361 attatgccat gcaacagctt gataagcgtg atagtacggc tatggcgggg gttgcctggt
      421 atcaccacga gcgttgggga accgtgaaag cctctgcggc tgcggacgtt ctggataaca
      481 gcaacggctg ggtgggggag ctatcggtat tccacaaaat gcagataggt cgcctgtcgc
      541 tgacacctgc gctgggcgtt ctctattatg acgagaattt cagtgactat tactatggca
      601 tttcagagag tgagtcccgt cgtagcggtc tggcaagtta ttccgcgcag gatgcctggg
      661 tgccctatgt cagcctgacg gcaaaatacc cgataggaga gcacgtcgta ttgatggcga
      721 gcgcaggata cagcgagctg ccggaagaga ttaccaacag cccgatgatt gatcgtaatg
      781 agagttttac ctttatcacc ggcgtgagct ggcgttttta attcaccggt ggatgtcggt
      841 gcggctggtg ctccgtcgta atgccacaaa ggttgttgct ctggattgct ctgttcatac
      901 cgggaacctg gcgtctgaat caggatctta tggctgaaca atcccttgcc ttcaaatgtc
      961 gtaaatggcg catcggcggg cagcaacagg ttatgcagtg actcgacgtt gtcggcctgc
     1021 tccatgcaat acggtttaat gacgttggct atttccggca gatttttcgc aagcacaccg
     1081 gtttaacgcc agcagcgtgg aagcggcgtt acagcaagga acatatcaat tcgtagtgcc
     1141 ggggcgatga agccccggcg tgagggatta ctgcccgtaa taggcattgg ccccatgctt
     1201 acgcaggtag tgcttatcca gcagttcgtt ttgcatcgca gggagctgcg gcgcaagctg
     1261 gcgcgagaat aggcccatat aggcgcattc ttcgagtact acggcgttat gcacggcatc
     1321 ggcggcgttt ttaccccatg cgaacgggcc gtgagaatgc accagcaccg ccgggatttg
     1381 tgccggactc ctgccacgtt cttcgaaggt ttcaatgatc acttcgccgg tctgatattc
     1441 atattcgccg ttaatctcct ctgcggtcat ctgtcgcgtg caggggatgg caccatagaa
     1501 ataatcagcg tgcgtggtgc cccaggcggg gagatccagc ccggcctgcg accagatggt
     1561 ggcgtggcgt gagtgggtat gcacaatgcc accgatttcg gcgtagcgac ggtaaagcgc
     1621 cagatgcgtt ggcgtgtcgg acgagggctt tttgctacct tccaccacct tgccgctggc
     1681 tatctcaacc accaccatat cgtcggcggt catcacgtca tactcgacgc cggaaggttt
     1741 gattaccatc cattgccgcg tttcgtctac cgcgctgaca ttgccccagg tgaacgtcac
     1801 cagatggtga gcgggaagcg ccagattcgc cgccagcacg tcggctttca gttgctctaa
     1861 catataaatc cagcctcctg catacgcgct tcaatccaac gccgcgcctg aataatctcc
     1921 agcaccggct ctttggcttt ttcggtccac atctcaatca ggaaagaacc gcgatagttc
     1981 agtttatgca gcgttttgaa gatgccaacg aaatcgacgc agccttcgcc aaacggcacg
     2041 tcgcggaact gtccgggact ttgctcggta actggctggg tgtctttcag gtggatcgcg
     2101 gcgatacggt caatgcccag tttcagttcg gcgggaacat cattgcccca ggcgctgagg
     2161 ttgccgacgt ccgggtagac ggtgaaccac ggcgaggcga gcatctcgtc ccactttttc
     2221 catttactga tggagttcat aaacgcggtg tccataatct ccaccgctag catgacttgt
     2281 gatgccgccg cctgttcgac cgcccacgcc agcccttcag caaaacgttg ccgggtgcct
     2341 tcatcgtggt cttcgtaata gacgtcgtag cccgccagct ggatggtgcg tatgccgaga
     2401 tcgcgtgcca gacgaatcgc tttgctcatg atctcccgtg ctcgctggcg taccgcgtcg
     2461 tcacggctac caaaaggaaa gcgacgatgt gcggacaggc acatcgacgg gatcccaaca
     2521 cccgtttcga tcatcgcggc aaccagcgaa gtcctttgtg cggtgctcca gtcaagacgt
     2581 gaaagccgtt cgtcggtttc atccaccgac atttcgacaa aatcaaaacc gcagcttttt
     2641 gccagcacca gccgctccgg ccaggagaga tctttcgcca gcgccttttc ataaatccct
     2701 aacggatgat tacgcacgct tgcctcccca gatagcgtcg atttgcgcat ggaaatcgcc
     2761 agccacctgc gccggatttg ctgagcctgc cagtgctcgc ccggcaataa acgctttcac
     2821 gcgaatatct ttaaacagcg gcaggtcagc aggggtaatc ccgccggtaa tggaaagctc
     2881 aaggccgata tcggaaagcg ccttcatgcg tgccagatcg gcttcgcccc actgttgccc
     2941 gctggcctgt gcatcacgac cgcgatgata aatcgcctgc cgcacgccaa tacgatgcca
     3001 gtcgcgggcg tcgtccagcg tccagttacc gaacagctcg atctgaattt caccaccgca
     3061 gcgttgcgcc atcgcgtggc ctttttccac cgtcgcgagc ggtgcggcgc agatgatggt
     3121 catccagtta gcgcctgcgc caaatgcctg ttgcgcgagc gtttcaccag cgtcggcgac
     3181 cttccagtcg gcaacgatga ttttgtcagg gcactgttcg cgcaaggctt tcaccgcgcc
     3241 aagcccttcg tttaaacaga gaatggtgcc cgcttcgaca atatcgacgc tgtcttttaa
     3301 ctgcgtcacg tcgcgctgtg cggcttcaag tgatgagtgg tcgagtgcca gttgcagaag
     3361 tggtcggctc ataatatgtg ctccttgatg cgggcgtgaa agccctgaag tgcggcaatg
     3421 agatgctgat agcgttggta tttttgttgg taaagctgat ggacagtcat atccggcagc
     3481 agggtgcgca ccgggtgctg taaatcacgc tgggcttcgc tgaaatcgcg ataaaccccg
     3541 gtgccgacgc gggcggcaag tgctgcacca aagcagccgg tttcttccac ctgcggcagc
     3601 tcaatacgca gaccgctgac gtccgccagc atttgcatcc agacatcgga atgcgccggg
     3661 ccgccagtga cgcgcagggt gtgcacatca gtaaaacgtt cgcgcatccg gttgagatgg
     3721 gtcatatggc tgaacaccac cccttcataa atggcctgca acaggtgtgc gcgggtgtga
     3781 atggcctgca tcccgtagaa accgctggtc atctcgagtc cggcgttgct gccgtacagg
     3841 aacggcagga aaaagagatc gccaccggcc ttcggcaagc tggcaacggc ctggttgatc
     3901 tcatcaaacg agatttctcc ccactgtgcg gtaaaccatt cgaggttgcc ggaagaggtg
     3961 gggctggctt cgtgaacgat aaattcacca tcgttaacgt agcgaccata gacatacgga
     4021 tgcgcttcac cgtcacgtaa accgcgggtt atgccgctgg tcaccgccca ggtgcccatc
     4081 accgcattaa gggtaaattc gtcttcgatc ccggcgcaga gtgcggtgga aaccacatca
     4141 aacaggccgc cgacaacggg cgtacccgct ttcagaccgg tcagtacggc tgtctgagcg
     4201 gtgatctccc cgcagatttc ggcagatccg acaacaggcg gcagggtgtg attgatttca
     4261 gcaatcccca gccagtcggt gaggcacggg tcatattccc caagactcat gttgtagaga
     4321 ttggactcgg aaatattgct ctcttcacag cctttgacgc cggttaaaca ccagcgcagg
     4381 tagtcgtgcg tcatcatcac gcagccaatt tgcgcgtagc gttccggttc gtgctcttta
     4441 agccaacgta acagcgatac cggatgcccg gtccacaagg tttgtcgggt cagcgggtag
     4501 agtttttccg ggatgccatc ttcctgccag cgacgaacga tttccatcgc ccggcgatcc
     4561 gaggataaaa tggcgttacc gagcggcttg ttgtttttat ccagcaaaaa caagcctttt
     4621 ccttgtgcgg agataccgat accgacaatt tgttccccgc taacgccgga atgggtaagc
     4681 agttcgcgaa tgacagccat gcagcattgc cacagttctg ccatatcgcg ctctgcccaa
     4741 cctggctgcg ggcttaatgc gcacagcggc aggcgctgca cgcctgcctc ccggccttcg
     4801 cggtcataca gcccggcttt cagccagcta ccgccacaat ctaaccccag ccagtattgc
     4861 gtcatggttt attgcacctc atccacggct ttcagtagat cagcaccctg cggaacatct
     4921 tttacaaaca tgtcgcggac ctgagtaccc agtgcgtcgc taaaggcttt gcggtcgaga
     4981 tcggtgatga cttccacgcc cgcttctttc atgccgtcga tgattttttg ctgatcttcg
     5041 gcaaccagtt tgcgctggta gttacccgct tcctgcgcag atgaaattag cgcttgctgg
     5101 aactccgggc ttaagccatc aaacttcgct ttgttgatca ccaccagaag cggggaatag
     5161 gcgtggtgcg tcagagaaag gtacttctgc acttcgaaaa attttgctga ccagacgacg
     5221 ttgatcgggt gttcctgagc gtcgatagtg cgggtttcca gcccggtata gacttcggca
     5281 aacggcatcg ggatcgggtt agcgccaaag actttgaatg cggcgatatt catcgggcta
     5341 ttgttggtgc gaattttcag ccctttcagg tcggcggggg ttttaaccgg tgcgcgcgag
     5401 ttggtgacat cgcgccagcc gttttcccag taggccagta cttttagtcc tttaccttca
     5461 agtgaggctt tcagatcatc accgactttg ccgtcgagcg ttttatgcgc gtgagcggta
     5521 tcgcggaaca ggaaagggac atcaagcaag ttcatcactg gtgataatcc ggcaaagtta
     5581 ttcgagccgg acatttccat atcgatggtg ccgccacgta cgccgctgat catcgcctgc
     5641 gcgttaccga gggtgctatc cggaaacaat tttaatttca gctcgccttt ggttctctcc
     5701 tgcaataaat cattgaattt tttcgccgca atatgttgcg agtcggtttg tgaggtttca
     5761 taaccgaaac gtaaagattg cgccgccaga gaagatgtgc tgaatgcagc caggccagca
     5821 atgaataatg cgtaggttac agagcgtaat ttcataatat tttcctttta attaatccat
     5881 ttcaaaggga ggatgatgag atcgggaata aaaacaaata cgactaataa tgaatataaa
     5941 accaggacgt aagggaaaac gcctctgact gcatcatcga atttgagttt tgccacccct
     6001 gaaataacgt taagtacatt accgataggc ggtgtaatta agccgattga gcagttaatg
     6061 ataaacatga caccgaaata aatcggatcg attcctgcct ctttaactaa aggcattaat
     6121 accggggtaa gaattaatac cgtcggcgtt aaatccatga ccatgccgac aatcaggata
     6181 gccaccataa tgacgataaa cagcagacgc ggtgaatcga ccaacggttg cagtaaatca
     6241 gaaaccatca tcggcagttc agcaatggta atcagccagg cggaaacttg tgccgaggcc
     6301 accagaaaca taaccactga ggtggttttc gccgcgccaa tgagtacatg ccaaagtgtg
     6361 gcgaaggtca tttcacggta aataactgtg gcgacaaaca gcgcatagaa agcagcaacg
     6421 gccccggctt cggtaggggt aaacagaccg gagcgaaacc cgccaataat gatgactgga
     6481 agaaacagcg cccagatacc ggagacaaaa gagtgccaga tttcctgcat cgttgctttt
     6541 tgctggcggg gtaaattaag gcgactggcc tgccaccacc aggtaagcat cagcgttgcg
     6601 cccatcatca tgccaggggc aatgcccgcc ataaacagct tactgatgga taatccgctg
     6661 gaaacgccga agataataaa cggaatggaa gggggaataa ttggcgcgat aatgccgcca
     6721 gaggcaatca gccccgccgc ccggttgacc gggtagttgg cgctgcgcat catcggcacc
     6781 agtagagcgg caacggcggc ggtatccgca acggcagaac cagaaaggct cgccataatc
     6841 atagctgcca gcacgccgac gtagcccagc ccgcctggtt tatgccccac cagtttcatc
     6901 ggcaggtcaa caatgcgttt tgacaagccg cccgcattca tgatttcacc cgccagcaca
     6961 aagaacggaa tcgccagcag ggagaagcta tcggctccgt tcaccagcgt ttgtgccatg
     7021 atctggacat caaacatgtc cagccagaac attaacgccg ccccgcacaa caacagtgcc
     7081 caggcaatag gcaaaccgat agcaatacca cccaacagac agcccagaaa aatcagcaca
     7141 gccatgatta agctccttgc ggtggcgacg ttagagaatt gctacgcgtg atgagttgat
     7201 ataaatgacg cagttcaaag aatgcgataa cgaggctggt gggcagacaa gcggcataca
     7261 tcaggccgat gggtaaaccg aggatcggtg aataatcgct ccagtcctga attgttttta
     7321 gcgttgcgcc ccaggccagt gcgccacaaa taaataagat taaggaatgg gtaaccagag
     7381 cgactcgtcg ttgccatgcg ggggagagtt tctccaccag aaaggtgacc tgaacgtggg
     7441 cgttatccat aaaagccaca atcgcgccaa taaatgttaa ccagacaaat aaataacgtg
     7501 acaattcatc tacagataaa atgcttgtct gaaaaccata tcttaaaata atgtttataa
     7561 acacaataca ggaaagtacg gcgagattaa tcgccagtat tgcttcaagt atttttttca
     7621 tagctattcc ttgaggctat gtctgtcata attcaagttg catgtgcagc aacctaaatt
     7681 attaatatag ttagagcaat aacattaccc agtcgttctg gcgtagacga ttattcgatt
     7741 aattcagcgc cgttaatgcg actttaacca caatttttcg tatctcagag gctgtttgca
     7801 aaatacaacc tggtcggtga acatcctgcg gaaagaaaat tgcgtagctg cccggtatca
     7861 tttctataaa tgattcatgt tcactgtcat gataaaaaat aatatcgcgc tgctctaata
     7921 gtgattcgct gactttatta tttcccgtat caatagcaat gccgattttc tcttcacccc
     7981 acgccagaaa ctgaatatcg atataccgac gatgcacttc cggacggttt tccaccgctt
     8041 cgcgtgtggt taaatcgata atttgcgcat aaatattttt gccgtcgatt tcgacaacgc
     8101 ccggctccag ggcgttgaaa tcggtagcgc gcagaaaatc gagcgccttt tcaatggcgg
     8161 cgggcaaacg gcacggattg ggctgcgcga tatgtccaaa aatcatgact tatctcctta
     8221 taacgcctgg attttggccc acacgctgtc atcaacggta ataccgttac ggcggttttc
     8281 ggccagcagg gtagtaaatt cgtggccggg taagcggatg gcctggtttt cgtcagcgcg
     8341 ttcagcggta gtaacgtaat ccatgatacg ttgcagcttg gcatcgcggg tgggaccgtc
     8401 gataagcttg tccacttcaa tggcaataaa aatttgtgaa atgccgtatt cgtcgctgtt
     8461 gtcctgggtg acttcggcaa ccgatgcgcc gtcggaaagg agagtagcga tcatatccag
     8521 cacaatcgac atgccagaac ctttccagta gcccatcggc aaaatgcggc gattcttctc
     8581 gataacgcca ggttctttgg tcaaattgcc ctcatcatca aagccaccat cgaccgggag
     8641 ctgacggccc gccaggcggt taacttctaa catgccgtaa gagaacatcg acatcgacat
     8701 atcgaccatg gtgatcggcg tggaaggaat ggcgacgatc agcgggttag tgccaatgcg
     8761 acactctttt gcgccccacg gcggcattac ggcgatggag ttggtccagc aaatgccaat
     8821 atagcctttt tccgccgcct gccagccgta gctgccgccg cgcatccagt ggttggcatt
     8881 acgaagtgcc accagaccaa taccgtgatc ggcagccagt tcaatggcgc gatccatcat
     8941 ctttttcgcc gtcaggttgc cgatcgaacg ctgggcatcc cactgttcaa ttgcgccgag
     9001 gctggttata cgtttgggtt gggcatcagg aatgatatcg ccgttttcca gttgttgaat
     9061 gaaacgaggg aaacgattaa cgccgtgaga ataaacgccg gattcggtgg tgcgggcgaa
     9121 catctctgca caggcgtcag ccgtttcgct gtcaacgccg cgtgaaatta agacccgatt
     9181 aaaggctgct tttaactgct caaatgtcac tttcatccca ggcttccttg tttttttgat
     9241 cgcttttttg gcttaaaatt tcaatatgcg aaacttgatt tcaaatatat cgatcacttt
     9301 ttaacaaagc aatctgattg atgaatttca aaagacataa aaatcaagtg gttataaatt
     9361 atctgtcgag atcgtgaact acggcacact ttgcgctacc atcaggacgc gacaaaatgg
     9421 ggaaagaagt gatgggaaaa aaagagaacg agatggcgca ggaaaaagag cgtccagccg
     9481 gaagccagag tctgtttcgc gggttgatgc tgattgagat tttgagcaac tatccaaacg
     9541 gttgtccgtt ggcgcatctt tcggagctgg ctggtttaaa taagagtacc gtccatcgct
     9601 tattgcaggg attacagtcc tgcggctatg tgaccaccgc gcccgccgca gggagttacc
     9661 gcctgactac caaatttatt gccgtcgggc agaaggcgct gtcttcgctg aatatcattc
     9721 atatcgccgc tccgcatctt gaggcactga acatcgccac tggtgaaacc attaacttct
     9781 ccagccgcga agacgatcac gctattttga tttataagct ggaacccaca accgggatgc
     9841 tgcgaacccg tgcctatatt ggccagcata tgccgctcta ctgttccgca atgggcaaga
     9901 tctacatggc gtttggtcac ccggactacg tgaagtcata ctgggaaaac catcagcatg
     9961 agatccagcc gttaacccgc aataccatta ccgagctgcc cgcgatgttc gacgaactgg
    10021 cgcacattcg tgaaagcgga gcggcgatgg acagagaaga aaacgaactc ggcgtctcct
    10081 gtattgctgt tccggtgttt gatattcatg ggcgggtgcc gtacgccgtg tcgatttcgc
    10141 tttcgacatc acgtctgaaa caggtgggag agaaaaatct cctgaaacca ctgcgtgaaa
    10201 ccgcgcaggc tatttctaat gaactgggat ttactgtcag ggatgacctg ggcgcgatca
    10261 cataacgctt tggacaagtg ccaaaacttt aacatttcct tcgttggatc aaagcagtag
    10321 ggacgcgctc tctggcactc tgctgtttta gtgcaaagga gtgatcatga accggtttat
    10381 tattgcggat gcgacgaaat gtatcggttg ccgtacctgt gaagtggctt gcgcagtgtc
    10441 gcatcaggag aatcaggatt gcgctgcgtt gtcaccagac gagtttattt cccgtattcg
    10501 tgtcattaaa gaccactgct ggaccacggc agtagcctgt catcagtgtg aagatgcacc
    10561 gtgcgcgaat gtctgccctg ttgacgcgat aagccgcgaa catgggcata ttttcgttga
    10621 acaaacacgt tgcattggct gtaaaagctg tatgctggct tgcccgtttg gtgcgatgga
    10681 ggtcgtttct tcgcgcaaaa aggcgagggc gattaagtgc gatctgtgct ggcatcggga
    10741 gacgggaccg gcctgtgttg aagcctgccc gacaaaggcg ttgcagtgca tggatgtcga
    10801 gaaagtgcag cggcatcggc tacggcagca gcctgtttga aacgtttttc cggcgtttct
    10861 caacgccgga atagtgcgct ttgtttatgc cagatgcggc gtaaacgcct tatccggcct
    10921 acaaattctt ccaaattcaa tatattgcag aaatcatgta ggcctgataa gcgtagcgca
    10981 tcaggcaatt tttagtgact ttcagcccag gctctttcta tctcttccgc cagaatcttc
    11041 acccccgcct caattttctc cggctctggt acgtagttca tgcgcataca ttgatgcgta
    11101 tgcggccacg gtttatccag ccctgggaag aagttgtgcc ccggcaccat cagcacgccg
    11161 cgtgctttca ggcgctgata gagctgctcg gtcgtaatgg gcaaatcctt aaaccatagc
    11221 cagaggaaaa tggctccttc cggtttatga atcaggcagc gattttccgg taaatagcgg
    11281 cgaatgatgg cgatagtttc ctgaacacgc tggtagtaaa acggtttgat gactgtttca
    11341 gacaggcgca gcagatcgtt acgcttaatc atttcacaca tcatcgccgg accaataccg
    11401 ccaggtgcca ggctgataat gccgttcata ttggtgatgg cggtgatgat tttttcattg
    11461 gcgatgataa tgccgcagcg ggagccaggc agccccagct tggaaagact catgcacagc
    11521 acgatattcg gattccatag cgggcgcgct tcactgaaga tgatacccgg gaacgggacg
    11581 ccataagcgt tatcaatcac cagcggaatg ccgtgttgat tcgccagcgc gtcaagcttc
    11641 agcaactctt cgtcagtaat cacattgcct gttggattcg tcggccggga gacgcaaatc
    11701 atcccggttt cttcgccaat atgcagatgc tcaaaatcga cgtggtattt aaactggcct
    11761 tccggcagca gttcaatatt cggacgcgca gagacaaaca gatcttcttc cagtccggcg
    11821 tcagcgtagc caatgtattc cggtgccagc gggaacagca cttttttgac ccgaccatcg
    11881 gcacggcgtc cggcaaacag attaaataag tagaaaaacg cgctctggct gccgtttgtt
    11941 agtgcaatat tctgtggttc gatatcccaa cccaacttct cgcgcagcat tccggcaagc
    12001 agtgtgagta gctccgtttt cccctgtgga ccgtcgtaat tgcacagtgc atcagtcgct
    12061 ttgccacttt ccagcatgtc ggtcagtagc gtctggaagt agtcctgcat ttccgggatc
    12121 tgcgccggat taccgccgcc gagcataatc gcgccagggg tgcgtaaacc gtcgttcaga
    12181 tcttccatca gcagcgtaat gccggagtgg cgggtaaatt tgtcaccaaa aagggagaat
    12241 gtcatagcgg gatatctgtc gaactctgta gcaaggaagg taacaataac gctacatttg
    12301 ctggggtgca aatcggtctg ttgaagagtg agcggtgctt tatcctgcaa cgctgattag
    12361 ggctgacatt ttatcctggt tggcggcttc ccgaggtact gacgaacggg aagccggaaa
    12421 agtcactgtt gcccagccca gatgaccagc actttatcgt cgccatgctc acgaacaaag
    12481 ccgtagccct gcttcagcga aagtgtcgtt tgtttgcccg cgccaattgc gggatggcgg
    12541 gcgcggaact ggctgatttt ctgccagtgc gcgacgttgg cggcagattt accgctaaca
    12601 tcctgccagt tcatatccga acgtgtacct tgcagcggat cagaacctgt aggaccgaac
    12661 ggacgcgagg attcatcgcc ataaaagatt tgtaccgcgc ctggcgctaa tagtaataac
    12721 tctgctgctt tgtcgccccc ttcacggaac agacgggtat catgcgacga gaggtagctc
    12781 aacacgttga aaccctgcaa tttctccgcc atttgctgcc aggtcatatc catctgcgca
    12841 atacaatcga cagctttcgc cgcctgctcc tgataatcga aattgatcat cgcatcgaag
    12901 ccgtggcgat agtagtcact ttgcatcacg ccgtggcccc aggcttcacc ggtcatccag
    12961 aaaggtttgt catctaatgc tttgtcgggg ttagcttttt tccattcgcg aagcgcggcg
    13021 ctggcttcgg ttttcagttg ctgccaggcg ggcaactcaa catgtttggc ggtatcgacc
    13081 cgaaaaccat caatcccata gtcgcggacc cactgactta accagtgggt taagtaatcg
    13141 cgaggggtaa agccgtcgat ggctttagcg tgggtatccg ttttgttttt atagaacacc
    13201 ggcagaccag aagcggtagt tgattcggtt ttgatatccg gcaaaaaggc tagcgacatg
    13261 gtgagatcgt cgaatccagg attgtcgtaa tcgccgatat cggtacggat ccagtttttt
    13321 ccccaccatt tatcccagcc tgttttgtcg ctgaaattaa tgtaatcgtt aaagctatgc
    13381 caggtttgcc cggcggcagg tttccagtcg ctccagcgtt cacccagcgt ttttttcacc
    13441 tcgtcaccag aaagatataa cgcgccaaac tgatactcct gcatatccgc cagcgtggca
    13501 tagccggtgt ggttcatcac gacatcaaag agaatacgaa taccgcgctg atgtgcgcta
    13561 tcaaccagcg tccgtagatc ggcttcgttg cccatattgg catcaagatt cgtccagtcc
    13621 tgtgtgtaat aaccgtggta ggcataatgc gggaaatcgc cttttgtacc gccgccgacc
    13681 cagccgtgaa tttgctcaaa tggggcgctt atccataaag cattaacgcc caactgctgg
    13741 aggtaatcca gtttgttggt caggccgcgt aaatcgccgc cgtgaaaagt gccaatttcc
    13801 gccataccgt ctttatgacg tccgtaactc tggtcattac tgggatcgcc gttttcgaaa
    13861 cgatctgtca gcacaaagta aaccgtggcg ttatgccagt cgaaaggggc agaggcgtca
    13921 gtttctgccc gttccagcag gagtaaaccg ttgctggtgg cagcgggttg taacgttatt
    13981 tgaccgttct tcactatcgc aatttgctgg ctgtaataat cccgtacggc ggctccttcc
    14041 gggaaagtgg cgctgacatc cagcgtgagc ggtaatccat cccatttcgg gcatacacgg
    14101 acctggcttg ctaccggttc ggcggcgttc tggatggaaa tcatcaatgt tggcgtgccg
    14161 gagcgggtgt ctatttgcag cgtatattcg ccgtccctga acaatcgcca ttgaggctgc
    14221 gtgttgctac aaggttgcag ggaaagcatc tgattgagtt ttatcgcatc cgcaggctgc
    14281 cagcactgtt ggtcaaaatt tagcgttagt ggacgcgtac ctttgggcaa ctgcgcgtgg
    14341 ctgacaaatg tttccgttcc ctgttcacta aaggcaggga accccggaga agtccagctg
    14401 gcggcaacgg cgaagccagg aaggagtgtc agaaaacagg cggcgagttt catggcggat
    14461 gagtccttat tgctgcgatt tcagacagtt tgccagcgat aagccaaaca aaactcatcc
    14521 ctgagcggta agtccacagg atgagaagca agggtgagcg atctcgcgca aaaaacggct
    14581 gaattttgcg ataaacccca cattttctgc gatttagcgc caatctgaat cgttaactcg
    14641 tgacatagtt tcagatttgg actattcctt ggggtgctct tgacagctat ttttgatatg
    14701 atttgagatt caactctcaa atttgtgaaa aatataaggt gttggaatga ttaaatccga
    14761 ccaggagacc tgatgatttt gactcccata cgacgatatg gggcgatgat tcttatgtta
    14821 ctcactctgg tgttttcgag tgaggtgtta gcgaagacgc acacaacaac agcgagtcaa
    14881 aagtcccact taactaaagc tagtaataaa caggtaagca gtaaacaaga gtattctcgc
    14941 aatagtgcaa agagtaattc acttcctgat ttgcgaaaat acccttccgg aacaccaagg
    15001 aaaaaggcgt ttctccggac cgtaatgcct tacattacca gccaaaatgc agccattact
    15061 gcggaacgta actggctgat ttcaaaacag tatcagggcc aatggtcacc tgctgagcgt
    15121 gcgcgtctga aagacatcgc caaacgctac aaggtgaagt ggtctggtaa tacgcgaaaa
    15181 atcccgtgga ataccttgct tgaacgcgta gacattatcc ccaccagtat ggtggcgacg
    15241 atggctgcag cagaaagcgg ttggggaacg tcaaagctgg cgcgcaacaa caacaatctg
    15301 ttcggcatga aatgcatgaa aggacgttgt accaatgcgc cgggtaaagt gaaagggtac
    15361 tcacagttta gttctgtcaa agaatcggtg agcgcctatg tcactaacct caatacgcat
    15421 ccggcttact cttcattccg taaatcgcgt gcgcagctgc gtaaagcgga tcaggaagtg
    15481 actgccacag cgatgattca caagctgaag ggctactcga ccaaagggaa gagttataac
    15541 aactacctgt tcgcaatgta ccaggataac caacggttaa tcgcggcgca tatgtaattg
    15601 catttccttg agccttatct gatttgtcgg tcggataagg cttttgctcg catcaggtgg
    15661 ctgtgctgag ttccctgatg tgacgccgac aattctcatc atcgctacaa catgacctcg
    15721 ctatttacat cgcgatactc ttttggcgtt gtgtcatatg cttttttaaa aacagagtag
    15781 aaatattgca gcgatggata accgcacatt tgcgatatct cattgatcga caaggtggtt
    15841 gaaatcagca gactgcgcgc tttctccagc ttctcggcat gaatcattgc atggatggtt
    15901 tcacccacct cttctttaaa acgcttctca agattggagc gcgagatccc gaccgcatcc
    15961 agtacctgat ccactttaat ccctttacag gcgtgattac gaatgtaatg catggcctga
    16021 ataacggcgg gatcggtcag cgagcgataa tctgttgagc gccgttcaat gacgcgaact
    16081 ggtgggacca aaattcgctg tagcggcatt tcttctttat ctaataatcg atgcaacagt
    16141 tttgccgcct gatagcccat ttgccgcgcg ccctgagcga ccgaagaaag ggcgacacgc
    16201 gacagatagc gggtcagttc ttcgttatcg atgccaatca cgcataattt ttccggtacg
    16261 ggaatatgta gatgttcaca tacttgcaga atatgccgcg cccgggcgtc agtaacggca
    16321 ataatcccgg tttgcggtgg cagcgtttgt agccagtctg ccagccgatt ttgcgcatgt
    16381 tgccagttct ctggcgcggt ttctaacccc tgataaacca ctccgcgata cttttcttcg
    16441 gcgacaagct gacgaaatgc atattcgcgt tcagtggccc aacgtttgcc gcttgattcc
    16501 ggaagaccat aaaaagcaaa gcggttaacg cctttctctt ttaaatgcaa aaatgcgctt
    16561 tcaaccagcg catagttatc ggtggcaatg taatgaacgg gtgggtaact ttctgcaagg
    16621 tgatacgagc cgccaacccc aacaatgggg acgtcgacat cagccagcgc ttgctcgatc
    16681 tgtttgtcgt cgaagtcggc aatgacgcca tctcctaacc agtccttgat tttatcaatg
    16741 cgggcgcgga aatcttcttc aatgaaaata tcccattctg attgtgatgc ctgtaaatat
    16801 tcccctacgc cttctactac ctgccggtca taggctttat tggcattgaa cagtaatgtg
    16861 atgcggtgac gtttagtaaa catggttctt ttcctgctga atcatgcaaa aactcaaaac
    16921 cggtaatacg taaccggctt tgagaaaatt tttatcaaaa tcaagaacgg cgtttggttg
    16981 cggagtccat ccatactgcc agcaacagaa tcgcaccttt aacgatatac tgccagaagg
    17041 tcggtacatc catcatactc atgccgttat ccagtgaagc catgataaat gcccccatta
    17101 ctgctccggc aacgcttccc acaccgccag ccaggctggt gccgccaatc acgcatgctg
    17161 caattgcgtc cagttcggcg atatttcccg cagaaggtga accagcgcca agtcgagaac
    17221 taaggattaa tccggcgatg gctaccatta atccgttaat cgcgaacacg gcaagtttgg
    17281 tgcgttcaac gttaatcccg gagagacgtg ctgcttccag attgccgccg atggcataaa
    17341 tgcgtcgtcc aaatgccgtc cgcgttgcca taaacattcc gccgagtaac agcaatgtca
    17401 gcagcagaac aggagtggga acgccacggt aatcattcaa cagccagatt gcgcctaata
    17461 cgatgatagc ggttaaagcc tggcgaccga ctactgcggt agaggccgga gactgcaaac
    17521 ccaaagcctg acggcgcatt cttccgcgcc attgccaacc aacaaaagcc attaagccaa
    17581 gcgcgccaat gatgaagcca gtgctggcag gtagatagct ttgcccaatt tgtgacatcg
    17641 cggcgctggt gggggaaaca gtcgtgccgt tggtgatgcc aatgagtatg ccgcgaaatg
    17701 ccaacatgcc cgcgagggtg acaataaatg aagggacttt gcggtacgcg acccaccatc
    17761 cgttccaggc accgagaagc agtcccagaa ccaacgtcac aatgatggta agtggcaaag
    17821 gccagcctaa ccagacgtca caaatcgccg cgacgccacc taatagcccc atcattgagc
    17881 cgacggaaag gtcgatttca gcagaaatta tgacgaacac cattcctacc gcgaggatgc
    17941 cggtaatcgc ggtctggcgt aacaggttgg agacgttacg ggcgcttaag taggcaccat
    18001 cggtggtcca ggtaaagaac agcatgattg cgatgatagc tgcaatcatc acgaagacct
    18061 gcaaattcag tgatttcagc ccggaaaagc caccggatgt cggtacggcc aatttcactt
    18121 cagacggatt gcttttcgac atgatgttcg ctcctcaatg cggcttccat cacctgctcc
    18181 tgagtcaggt tatgatttat caggttggct tttagtttcc cttcatgcat caccagtacc
    18241 cgatcgctaa ggccgagcac ttcaggtaat tcggaagaga tgacaataac ggcaataccc
    18301 tgctggacga gttggttaat taatttgtag atctcgtatt tcgcgccaat atcgataccc
    18361 ctggtaggtt catcaagaat gaggatgcgc gggttaagta acagacagcg agcgaggatc
    18421 gctttttgct gattgccgcc gctcaaacgt ccaatagcaa gatcggggga cgacgtttta
    18481 actttgagtt gctgaattga ttccagaata catttttgct cttccgcgtc atcaagctgg
    18541 ctaatgccac cggtaaattt attgagtgcg gcgagggtaa tatttttacc aaccgccatt
    18601 accggaacga tgccgtcgcg ctttctgtct tcgggtacca tcgcaatccc ctgggcgatg
    18661 gcttgctgac agttacgaat atctacctgt ttgccatcaa tataaatttt tccttcccat
    18721 tgtccgggcc acacgccaaa caggcactga atggtctcgg tacgtccggc accaacgagt
    18781 ccggcaatac ccagtatttc gccacgtttc agggaaaacg agacatcatt aactcgttta
    18841 atatgacgat tgaccggatg ccatgccgtc agatgttcaa tacgtaatat ttcatctccg
    18901 gtggtatgtg gttcattagg gtaaagcgcg gttaactctc gcccgaccat catggtgata
    18961 atatcgtctt cactcattcc ggcagcatca cgcgtaccaa tgtgctgtcc gtcgcgaata
    19021 acgcaaatcg tatcggaaat cgctttgact tcgttgagtt tgtgcgaaat ataaatacag
    19081 gcgataccgt gctgttgtag atcgcgaata atatccagta aaaccgacgt ttcctgctca
    19141 gttaatgagg ctgtcggttc atcgagaatt aacaagcgca cctgtttatt aagtgccttg
    19201 gcaatttcaa ccagttgttg ttgcccaagc cctaaatcgc caacgcgggt atcaggtgaa
    19261 atggataaac tgacctgtgc gagcagcttc tgacagcgta gcgtcatcag gtcataatcc
    19321 ataatgccat tgtgggttat ttcgttaccc aggaagatat tttccagcac ggtcaattct
    19381 ttcaccaggg ccaattcctg gtgaatgatg gcgatacctt tgcgttcggt atcgcggatg
    19441 tgactcgcct gaatctcttc tcccgcaaaa ataatttcgc cttcgtagga gccatgggga
    19501 taaataccac acagcacttt catcagcgtt gatttaccag acccattttc cccacaaagt
    19561 gagacgattt cgccagcatt caaccgcaag cagacgttat caatcgcctt cacactgccg
    19621 aaggttttgg taatattctt catttcaagt agataaggca taacgactcc acctaagcca
    19681 attcattcac gcggcatgga gagaaatcac gcccccgctc cgcgccgggg cgtaacgctt
    19741 acagctcgct ctctttgtgg aatccgtctt taactaccgt gtctttgatg ttgtttttat
    19801 tcacatcgat cggtgtcagg aggcgggagg gcacatcttt caggccatta ttcagtgtgg
    19861 tatctgcttt tggttcctga ccattgccca actcaacggc aatttctgcg gcagtatttg
    19921 ccaacaacgt aataggttta tacaccgtca tagtttgcgt accggcagca atacgtttaa
    19981 tacctgcgag atccgcatcc tggccggaaa ttgctacttt ccctgataaa ccttgcgcgc
    20041 ttaatgcctg aattgcccca cctgcggtgg catcgtttga ggcaactaca gcatcaattt
    20101 tgttattatt ggcggttagc gcgttttcca taattttcaa tgcgttttcc ggtaaccagc
    20161 catcaaccca ttggtcacca acgactttaa tttttccgga atcaacgtaa ggttttaaca
    20221 ctttcatttg tccggcgcgg aacagcttgg cgttgttatc taccggcgag ccgcccatca
    20281 ggaagtaatt accttgcgga acaatatcga ccagggcttt tgcctgcagt tcaccgactt
    20341 tttcgttatc gaaagaaata taaaaatcga tatccgcatc gttaatcata cgatcgtaag
    20401 ctaatacttt aataccttct tgtttggctt cttttacaac gttacttaat acctgaccgt
    20461 tatacggaat aatgacaaga acatcaacac cccggtttat catgttttca atctgcgaca
    20521 tttgtgtttc ttcattgcca tttgcagact gtacaaatac tttcgcgccg agagattctg
    20581 cctttttcac aaaaatatct cgatcttttt gccagcgttc aagacggaga tcatcaatcg
    20641 ccatacctat tttgacttct ttggcgtgtg ccgcaacgtt ggtaagcaga agtgaggtgc
    20701 aaagggtgag tagaatgttc tttattttca tggtgtaggg ccttctgtag ttagaggaca
    20761 gctttaataa gtaacaatca ccgcgataaa cgtaaccaat ttttagcaac taaacagggg
    20821 aaaacaatta cagattttta tctttcgatt acgatttttg gtttatttct tgatttatga
    20881 ccgagatctt acttttgttg cgcaattgta cttattgcat ttttatcttc gaggaattac
    20941 ccagtttcat catcccattt tattttgcga gcgagcgcac acttgtgaat tatctcaata
    21001 gcagtgtgaa ataacataat tgagcaactg aaagggagtg cccaatatta cgacatcatc
    21061 catcacccgc ggcattacct gattatggag ttcaatatgc aagcctattt tgaccagctc
    21121 gatcgcgttc gttatgaagg ctcaaaatcc tcaaacccgt tagcattccg tcactacaat
    21181 cccgacgaac tggtgttggg taagcgtatg gaagagcact tgcgttttgc cgcctgctac
    21241 tggcacacct tctgctggaa cggggcggac atgtttggtg tgggggcgtt taatcgtccg
    21301 tggcagcagc ctggtgaggc actggcgttg gcgaagcgta aagcagatgt cgcatttgag
    21361 tttttccaca agttacatgt gccattttat tgcttccacg atgtggatgt ttcccctgag
    21421 ggcgcgtcgt taaaagagta catcaataat tttgcgcaaa tggttgatgt cctggcagcc
    21481 aagcaagaag agagcggcgt gaagctgctg tggggaaccg ctaactgctt tacaaaccct
    21541 cgctatggcg cgggtgcggc gacgaaccca gatcctgaag tcttcagctg ggcggcaacg
    21601 caagttgtta cagcgatgga agcaacccat aaattgggcg gtgaaaacta tgtcctgtgg
    21661 ggtggtcgtg aaggttacga aacgctgtta aataccgact tgcgtcagga gcgtgaacaa
    21721 ctgggccgct ttatgcagat ggtggttgag cataaacata aaatcggctt ccagggcacg
    21781 ttgcttatcg aaccgaaacc gcaagaaccg actaaacatc aatatgatta cgatgccgcg
    21841 acggtctatg gcttcctgaa acagtttggt ctggaaaaag agattaaact gaacattgaa
    21901 gctaaccacg cgacgctggc aggtcactct ttccatcatg aaatagccac cgccattgcg
    21961 cttggcctgt tcggttctgt cgacgccaac cgtggcgatg cgcaactggg ctgggacacc
    22021 gaccagttcc cgaacagtgt ggaagagaat gcgctggtga tgtatgaaat tctcaaagca
    22081 ggtggtttca ccaccggtgg tctgaacttc gatgccaaag tacgtcgtca aagtactgat
    22141 aaatatgatc tgttttacgg tcatatcggc gcgatggata cgatggcagt ggcgctgaaa
    22201 attgcagcgc gcatgattga agatggcgag ctggataaac gcatcgcgca gcgttattcc
    22261 ggctggaata gcgaattggg ccagcaaatc ctgaaaggcc aaatgtcact ggcagattta
    22321 gccaaatatg ctcaggaaca taatttgtct ccggtgcatc agagtggtcg ccaggagcaa
    22381 ctggaaaatc tggtaaatca ttatctgttc gacaaataac ggctaactgt gcagtccgtt
    22441 ggcccggtta tcggtagcga taccgggcat ttttttaagg aacgatcgat atgtatatcg
    22501 ggatagatct tggcacctcg ggcgtaaaag ttattttgct caacgagcag ggtgaggtgg
    22561 ttgcttcgca aacggaaaag ctgaccgttt cgcgcccgca tccactctgg tcggaacaag
    22621 acccggaaca gtggtggcag gcaactgatc gcgcaatgaa agctctgggc gatcagcatt
    22681 ctctgcagga cgttaaagca ttgggtattg ccggccagat gcatggagca accttactgg
    22741 atgctcaaca acgggtattg cgccctgcca ttttgtggaa cgacgggcgc tgtgcgcaag
    22801 agtgcacttt gctggaagcg agagttccgc aatcacgagt gattaccggc aacctgatga
    22861 tgcccggatt tactgcgcct aaattgctat gggttcagcg gcatgagccg gagatattcc
    22921 gtcaaatcga caaagtatta ttaccgaaag attacttgcg tctgcgtatg acgggggagt
    22981 ttgccagcga tatgtctgac gcagctggca ccatgtggct ggatgtcgca aagcgtgact
    23041 ggagtgacgt catgctgcag gcttgcgact tatctcgtga ccagatgccc gcattatacg
    23101 aaggcagcga aattactggt gctttgttac ctgaagttgc gaaagcgtgg ggtatggcga
    23161 cggtgccagt tgtcgcaggc ggtggcgaca atgcagctgg tgcagttggt gtgggaatgg
    23221 ttgatgctaa tcaggcaatg ttatcgctgg ggacgtcggg ggtctatttt gctgtcagcg
    23281 aagggttctt aagcaagcca gaaagcgctg tacacagctt ttgccatgcg ctaccgcagc
    23341 gttggcattt aatgtctgta atgctgagtg cagcatcgtg tctggattgg gtcgcgaaat
    23401 taaccggcct gtgcaatgtc ccagctttaa tcgctgcagc tcaacaggct gatgaaagtg
    23461 ccgagccagt ttggtttctg ccttatcttt ccggcgagcg tacaccacac aataatcccc
    23521 aggcgaaggg ggttttcttt ggtttgactc atcaacatgg ccccaatgaa ctggcgcgag
    23581 cagtgctgga aggcgtgggt tatgcgctgg cagatggcat ggatgtcgtg catgcctgcg
    23641 gcattaaacc gcaaagtgtt acgttgattg gtggcggggc gcgtagtgag tactggcgtc
    23701 agatgctggc ggatatcagc ggtcagcagc tcgattaccg tacggggggg gatgtggggc
    23761 cagcactggg cgcagcaagg ctggcgcaga ttgcggcgaa tccagagaaa tcgctcattg
    23821 agttgttgcc gcagctaccg ttagaacagt cgcatctacc agatgcgcag cgttatgccg
    23881 cttatcagcc acgacgtgaa acgttccgtc gcctctatca gcaacttctg ccattaatgg
    23941 cgtaaacgtt atcccctgcc tgaccgggtg ggggataatt cacatctata tatctcacta
    24001 attaattaat atttagtatg aatttattct gaaaatcatt tgttaatggc atttttcagt
    24061 tttgtctttc gttggttact cgtaatgtat cgctggtaga tatggagatc gttatgaaaa
    24121 cctcaaaaac tgtggcaaaa ctattatttg ttgtcggggc gctggtttat ctggttgggc
    24181 tatggatctc atgcccattg ctaagtggaa aaggctattt tcttggcgtg ttaatgacag
    24241 caacttttgg taactatgca tatcttcgcg cagaaaaact cgggcaactg gataattttt
    24301 ttacccatat ctgccagtta gttgcgttaa tcactatcgg tctcttgttt atcggtgttt
    24361 taaatgcacc tatcaatgct tatgaaatgg tgatctaccc catcgccttt tttgtctgct
    24421 tgtttggtca aatgcgtttg tttcgctcgg tatgagcaac ataaagctct tacacattca
    24481 ggaatgaaag gaatactgtg atggacaaca aaatatcaac ctattcaccg gcctttagta
    24541 ttgtgtcatg gatagctctc gttggtggta tcgttaccta tctgttaggg ctatggaatg
    24601 cagagatgca gttaaatgaa aaagggtatt attttgccgt actggtgtta ggactgtttt
    24661 ctgctgcgtc ttatcaaaag accgttcggg acaagtatga aggcataccg accacttcca
    24721 tttattatat gacctgcctg actgtcttta ttatctctgt tgcattactg atggtaggtc
    24781 tgtggaatgc gacattatta ctcagtgaaa aaggttttta tggactggct ttcttcttaa
    24841 gcttgtttgg tgcagtagcg gtgcagaaga atattcgtga taccggaata aacccgccaa
    24901 aagaaacaca ggttacccag gaagaataca gcgaataatt cacgtaagcc cggtcagtcc
    24961 aatgtgaacg ggcttttact taactcacta atctgtttct gtcgattcgt tgtaccagca
    25021 tagaaagtaa caaactcgct gccaacgtcg cgcaaaagat ccaaataata tccagtattg
    25081 gccaattttt aagctcaatt ccccgggtgc gcagcgcatg gataatcaag gcgtggaatc
    25141 cgtatatacc caatgaatgg cgggagatta agccaagtcc gcgaatggta cgcgtatcca
    25201 gcgtgttttt aaccagagtc aatagcgcga ttgcgcagat aaaaaccatc ggcccacagt
    25261 aaagatacca ggtatcggca aaatttccgc gccactgcaa ttcatataat gtcccgcgag
    25321 agataataaa aacccccgtc gcaaacagcg cggcgctcac ccacgacagt gctttatgct
    25381 gtgtgtccat catccctata gcgcggccca acatgccata cagaatgtag taaaaagtat
    25441 cgccattgat atataagtta attggcagcc attcaaaacc gtcaattttc tgcggcactg
    25501 tgtttgggtt agcgataatg ccaatcacca ccattagcac cagcaacatt tttccgccga
    25561 cgttcttcac ctgaatcagc ggtgaaacca gataaatcac cgcaatcgcg aagaaaaacc
    25621 acaagtggta aaacactggc ttttgcagca ggtttttcag cgctaactcc atattgatgg
    25681 aggtaaacag cgcaatgtag agcagtgcga ttgcgctata aaaaatcaga cataagccga
    25741 tacgcaagaa atggcgcggc tgggcgctgc gttcgccaaa aaagagatag ccggaaatca
    25801 tgaaaaatag cggcacgctg acacgagagg cagaattcag aacattggcg atatcccatg
    25861 tgacggggct aacactatga gcattggtca cataccaggt agtggtgtga atcatcacca
    25921 ccattaaaca cgctatccct cgcaggttat caatccagta aattttgggc tgcatctgtg
    25981 tctctgtatc tggttaaaaa aagtctgacc gataaatcat ttggttggcg cactggaata
    26041 atctgagttt tatcactaca gcttatagag gcttaaggaa attcgtaaga tatcagccac
    26101 tataccgata taaataataa gactcacctg caaaccagac ggtaatttaa tgatgatgaa
    26161 cgctttcttt ccggcaatgg cgcttatggt gctagtgggt tgttctacac cgtcacccgt
    26221 gcagaaagca caacgggtaa aggttgatcc tctgcgttcg ttgagtatgg aagcgttatg
    26281 caaggatcag gcggcaaaac gttataacac cggcgagcaa aaaatcgacg tcaccgcctt
    26341 cgaacagttc cagggaagct atgaaatgcg cggttatacc ttccgtaaag agcagtttgt
    26401 ctgttctttt gacgcagatg gccatttttt gcatctttcc atgcgttaag ccctgctttt
    26461 tcccgtttcg tactgtatat cttccatcca gcgggtatac tgatcccttc ctttaaatcc
    26521 acacgtatcc agcacgaaat aatatgcaaa agtttgatac caggaccttc cagggcttga
    26581 tcctgacctt acaggattac tgggctcgcc agggctgcac cattgttcaa ccattggaca
    26641 tggaagtcgg cgcgggaacc tctcacccaa tgacctgtct gcgcgcgctg gggccagaac
    26701 cgatggcggc tgcttatgtt cagccttctc gtcgcccgac cgatggtcgc tacggcgaaa
    26761 accccaaccg tttacagcac tactatcagt tccaggtggt cattaagcca tcgccggaca
    26821 atattcagga gctgtacctc ggttctctga aagagctggg catggacccg actattcacg
    26881 acatccgttt cgtggaagat aactgggaaa acccgactct gggtgcctgg ggactgggct
    26941 gggaagtgtg gctaaacggc atggaagtga cgcagttcac ttacttccag caggttggtg
    27001 gtctggagtg taaaccggtt accggcgaga tcacctacgg tctggaacgt ctggccatgt
    27061 acattcaggg cgtagacagc gtttacgacc tggtctggag cgacggcccg ctgggtaaaa
    27121 ccacctacgg cgacgtgttc catcagaacg aagtggagca gtccacttac aacttcgaat
    27181 acgcggatgt ggacttcctg ttcacctgct ttgagcagta cgagaaagaa gcgcagcagc
    27241 tgctggcgct ggaaaatccg ctgccgctgc cagcctacga gcgtattctg aaagccgccc
    27301 acagcttcaa cctgctggat gcgcgtaaag ccatctccgt caccgagcgt cagcgctaca
    27361 ttctgcgcat tcgcaccctg accaaagcag tggcagaagc atactacgct tcccgtgaag
    27421 ccctcggctt cccgatgtgc aacaaagata agtaagaggc ggctatgtct gagaaaactt
    27481 ttctggtgga aatcggcact gaagagctgc caccaaaagc actgcgcagc ctggctgagt
    27541 cctttgctgc gaattttact gcggagctgg ataacgctgg cctcgcacac ggcaccgttc
    27601 aatggtttgc tgctccgcgt cgtctggcgc tgaaagtagc taacctggcg gaagcgcaac
    27661 cggatcgtga aatcgaaaaa cgcggcccgg cgattgccca ggcgttcgac gctgaaggca
    27721 aaccgagcaa agcggcagaa ggttgggcgc gtggttgcgg tattaccgtt gaccaggctg
    27781 agcgtctgac taccgataaa ggcgaatggc tgctgtatcg cgcccatgtg aagggcgaaa
    27841 gcaccgaagc actgctgccg aatatggttg cgacttctct ggcgaaactg ccgatcccga
    27901 aactgatgcg ttggggcgca agcgacgtgc acttcgtgcg tccggtgcac accgtgaccc
    27961 tgctgctggg cgacaaagtc attccggcaa ccattctggg cattcagtcc gatcgcgtga
    28021 ttcgcggcca ccgctttatg ggcgaaccgg aattcaccat cgacaacgcc gatcagtatc
    28081 cggaaattct gcgtgagcgc gggaaagtca tcgccgatta cgaagaacgt aaagcgaaga
    28141 ttaaagccga tgccgaagaa gcagcgcgta agattggcgg taacgctgac ttaagcgaaa
    28201 gcctgctgga agaagtggct tcgctggtgg agtggccggt cgttctgacc gcaaaattcg
    28261 aagagaaatt cctcgcggtg ccggctgaag ccctggttta caccatgaaa ggtgaccaga
    28321 aatacttccc ggtgtatgcg aacgatggca aactgctgcc gaacttcatc ttcgttgcca
    28381 acatcgaatc gaaagatccg cagcaaatta tctctggtaa cgagaaagtc gttcgtccac
    28441 gtctggcgga tgccgagttc ttcttcaata ccgaccgtaa aaaacgtctg gaagataacc
    28501 tgccgcgcct gcaaaccgtg ctgttccagc aacagctggg tacgctgcgc gacaaaactg
    28561 accgcatcca ggcgctggct ggctggattg ctgaacagat tggcgctgac gttaaccacg
    28621 caacccgtgc gggcctgctg tctaagtgtg acctgatgac caacatggtc ttcgagttca
    28681 ccgacaccca gggcgttatg gggatgcact atgcgcgtca cgatggcgaa gcggaagatg
    28741 tcgcggtggc gctgaatgag cagtatcagc cgcgctttgc cggtgatgac ctgccgtcta
    28801 acccggtagc ctgtgcgctg gcgattgctg acaagatgga tactctggcg ggtatcttcg
    28861 gtatcggcca gcatccgaaa ggcgacaaag acccgtttgc gctgcgtcgt gccgcacttg
    28921 gcgtgctgcg aattatcgtt gagaagaacc tcaaccttga tctgcaaacg ctgaccgaag
    28981 aagcagtgcg tctgtatggc gataagctga ctaacgccaa cgtggttgat gatgttatcg
    29041 actttatgct cggtcgcttc cgcgcctggt atcaggacga aggttacacc gttgacacca
    29101 tccaggcggt actggcgcgt cgtccgactc gtccggctga tttcgatgcc cgcatgaaag
    29161 cggtatcgca cttccgtacc cttgaagcag ctgctgcact ggcggcggcg aacaagcgtg
    29221 tctccaacat tttggcgaaa tctgacgaag tgctgagcga ccgcgtgaat gcctctaccc
    29281 tgaaagagcc ggaagaaatt aaactggcga tgcaggttgt ggtgctacgt gacaagctgg
    29341 agccgtactt tgcggaaggt cgttaccagg atgcgctggt cgaactggca gagctgcgtg
    29401 agccggttga tgctttcttc gataaagtga tggtcatggt tgatgacaaa gaattgcgtc
    29461 tcaaccgtct gaccatgctg gagaaactgc gcgaactgtt cctgcgcgtt gcggatattt
    29521 cgctgttgca gtaataacgc cgttattaaa aagcctgcca tctggcaggc tttttttatt
    29581 atcgctaaat aatacagcaa cctttaataa tcttctgctg aataaagatt atctcatata
    29641 ttaattttat gaggtttttt aggattatat caaggagaag aaacaaactt attaagctag
    29701 aatagccacg ggtgcttgag actgtttgtc tcaggtattc accgaaaggc agacagagaa
    29761 aagccccacc tgactataaa tcaaagtgag gctaccctat gcctgaacac cataaggtta
    29821 gcctcttact cgttggaaat caacacaggg ggctgggaat gccgcagaaa tatagattac
    29881 tttctttaat agtgatttgt ttcacgcttt tatttttcac ctggatgata agagattcac
    29941 tgtgtgaatt gcatattaaa caggggagtt atgagctggc ggcattttta gcctgcaatt
    30001 taaaagagta agagtcttcg gcgggaagtt attcccgcct ttcttacggc gttgcgcatt
    30061 ctcattgcac ccaaatttat ttttcacaaa aataatacta gattttatta cgcgatcgat
    30121 tatttatttc ctgaaaaaca aatagaaaaa tccccgccaa atggcaggga tcttagattc
    30181 tgtgctttta agcagagatt acaggctggt tacgttacca gctgccgggc ctttagcgcc
    30241 gctttcgatg gtgaaggaca ctttctgacc ttcgtccaga gatttgtaac catcgttctg
    30301 gatagcagag aagtgtacga acacgtcttt agagccatcg tcaggagtga tgaagccgaa
    30361 gcctttgtca gcgttgaacc attttacgat accagtcatt ttaccggaca tagtgtatta
    30421 cctttaataa ttaagtgtgc ctttcggcga tatggcgtgc tttacagatt ttgaagcgtt
    30481 aaaggaatgt gcactacgag gggtatcaac gataactctt gaagggactt gccttactac
    30541 actgctttaa tggtctgtac gtcaaaccgt tgatgtgcat taagccacgc attggcgggt
    30601 gatgcaacaa ttatttttca tatttatgat taatcggttg gaaaacggtc ctgtcatcag
    30661 gaccgtaaac agcaataaag tggataaaag tctattccat caactgctta cttaatgccg
    30721 ggttggcttg aatcaaacgc atcaatttta gttcggcact tgaaggcttc acgcgtctgg
    30781 attcccattc ctttaccatg gcgactgata cgcccaaaac ccgggcgaaa tcatcgattt
    30841 ttaaccctgt cccttttcgt aactgctcaa tttcggtaca ggacgttgtt ctgtgcgtca
    30901 gggtaatttt ctgcgtttca tctttaaaaa caatctgttc caggctgctc aacagctcat
    30961 gcattggatc tttatattcc attgaaaact cctcaaatca cactgcgggg tcgtgaattg
    31021 catcgaagct cattaagaat agtcgggaaa agcagacaca gcggggtgaa gtcagggcat
    31081 gctctggcta tgattcgatt cagccagtca tttagcgtga cttatagcca cctaacgatc
    31141 tgataaatgc cgatatatct cttaccactc ctggtttgta aagatttcga aaatctaacc
    31201 ttctgcgaag gcgtaaatct ctggctacaa aaaaagcaac ggcactatca ttttttaaca
    31261 atgaaacaac aaattaacct tgtttcattt tctccatgat tttaccagga agattacgta
    31321 gaaagggtat cctgcaagcc tgtcctggac tatccttgtc acgtcagaca cgcgtgtgtc
    31381 gttgtgcgct ttttttgggt gaaaggagta agaaaatggc gacaggaaag tcctgctctc
    31441 gctggtttgc gcctcttgcg gcgttattaa tggtagttag cctgagtggg tgttttgata
    31501 aagaaggcga tcagcgtaaa gcgtttatcg acttcctgca gaatacagtg atgcgtagcg
    31561 gtgaacgtct accaaccctg actgccgatc agaaaaaaca gtttggtcct tttgtctctg
    31621 attacgcgat tctgtatggt tattctcagc aggtgaatca ggcgatggat tccggtctgc
    31681 gcccggtcgt agacagcgtt aacgcaattc gcgtaccgca ggattacgtt acgcaaagcg
    31741 gtccgctgcg tgaaatgaac ggttcgctgg gtgtgctggc gcaacaactg caaaatgcga
    31801 agttgcaggc cgatgctgcg cactctgcgc taaaacagac cgatgatttg aaaccggtct
    31861 ttgatcaggc attcaccaaa gtggtaacga cgccagctga tgcattgcaa ccgttgattc
    31921 cggctgcaca aactttcacg caacaactgg tgatggttgg agactacatt gctcagcaag
    31981 gtactcaggt gagctttgtc gcaaatggca tccagttccc gacttcacag caggcaagtg
    32041 aatataacaa actgattgcg ccattaccgg cacaacatca ggcgtttaat caggcctgga
    32101 ctacagcagt tactgcaacc caataaagag taaaagcccg agcgattatc gggctttact
    32161 tcacgcagtc gcggcttagt ccgcgacgtg cggattcaca cagttcttct caacctttcc
    32221 ttgtaacgca tcaatcaaat tatccacggc acaggcggcc atgccataac gcgtctcatg
    32281 ggtggcagat ccaatatgcg gtactgcgac gacgttggcc attgagagca acggcgaatc
    32341 tacggacagt ggctcttgtt cgaagacatc cagcccggcg gcgtgaattt cgcctttctg
    32401 caatgcagca atcagtgcat tttcatcaac caccgggcca cgtccggcat taatgaaaat
    32461 ggcggaggat ttcattttgg cgaattgttc tgcgccaaac agatgatgcg tctcatcagt
    32521 taacggcagg atcaggcaaa cgaaatctga ctcttgtaac agagtatcca aatcgcagta
    32581 gcgggcgttg aagcgttctt ctgcttcttt atggtggcgg cgcgcgttat agaggatggg
    32641 catgttgaag ccaaagtgcg cacgttgtgc cagcgccata ccgatccgtc ccatcccgac
    32701 aatgcccagt gttttatggt gaacgtcagt gccgtaccag tccgggccta tgctcgcggt
    32761 ccattcgcct gcttttaccc gttctgctac ctccacaacc cgacgagcgg tagacaacac
    32821 cagcgccatc agcgtatcgg cgacggtttc tgttaatacg gttggcgtgt gcatcagcag
    32881 aatttttcgg gcggtaagcg catcgacatc aaaattgtca tagccgacgg agatcgttga
    32941 tgtggcacgc agtttcggca ttttttccag caatgcggca tcaacattct cgtttgaacc
    33001 cagtaaacct tcagcttcgg caaaaattgc tgcattttgt tcgacggttt gtgggctgag
    33061 gtttgccacc tggtgaacgg tgaaatgctc ttgcaggcgt tgcagtaaat catcaggtaa
    33121 ggctttgtag aggataacgg acggcttcat gcttctctcc attcactgat aaggcgatca
    33181 ccttagcctg aaatatgatg aaggaaaact gaagaatgcc aggtgatttc tgcatcacct
    33241 ggcatgaaag ggattacagc gggcttaagg taatttctac acggcggttt tgcgccttac
    33301 cttctgcggt gctgttgctg gcgattgggt tagccgggcc aaggccctga gtacggatgc
    33361 ggctggcgtc cacgccctgg gtgatcaacg cgctggcaac ggaatccgca cgttgctggg
    33421 agagacgcat gttcaggtcg tgaccacccg tgctgtcggt ataaccaatc acgttaaccg
    33481 ccgttttcgg atactctttc agcaccattg ccacgccggt cagggtgtta gcgcccgcag
    33541 gtttcagggt cgcgctgctg ctgtcgaagg tcacattgtt cggcatattg aggataatgt
    33601 tatccccgct gcgggttacg ctaacaccag tgccgcgcat tttgtcacgc agcttcgctt
    33661 cctgcacatc catgtaataa cccacgccgc cgcccagagc tgcgcctgct gctgcgccaa
    33721 tcagcgcgcc tttaccgcga tctttcttcg aagaagagag cgcaccaata cccgcgccca
    33781 cgagagagcc cagacctgcg ccgatagcag atttacctgc ttcgcgttcg ccggtgtaag
    33841 ggttagttgt gcagccagat accgccagag cgccactcac tacggcggca ataagataaa
    33901 cacgtttctt cattgttaat ccttaataac ctttttattc tttgccacgg gttccgtggc
    33961 gggagattat gccgcgtgaa catgaagatt attcctggga atactcggaa atttgtaagt
    34021 aatatttaac tgctcaatac atctaacctt tcaggagttt tcggtttggc caactcatcc
    34081 tcacgatatt ccgttctgac tgccgcccat tgggggccca tgctggttga aaccgacggc
    34141 gaaaccgtgt ttagctcgca tggcgcgtta gccacaggaa tggaaaactc cttgcagagc
    34201 gcggttcgcg accaggttca cagcaataca cgggtacgat ttccaatggt gcgcaaaggc
    34261 tttcttgcgt caccggaaaa tccgcaaggc attcgtgggc aggatgaatt tgttcgcgtg
    34321 agttgggatg aggcgctgga tcttattcac caacaacata aacgcattcg tgaggcttat
    34381 ggtccggcat cgatttttgc tggttcctac ggctggcgtt caaacggcgt gctgcataag
    34441 gcctcgacat tattacaacg ctatatggcg ctggcaggcg gttataccgg gcatctgggg
    34501 gattattcga ccggcgcggc acaggcgatc atgccgtatg tcgtgggtgg tagcgaagtt
    34561 tatcaacagc aaaccagttg gccgctggtg ctggaacata gcgatgtcgt ggtgttgtgg
    34621 agtgctaacc cactcaatac gctgaaaatt gcgtggaatg catctgatga gcaggggctt
    34681 tcttactttt ccgcactgcg tgacagcggg aaaaagctga tctgtattga tccaatgcga
    34741 tcggaaaccg tcgatttctt tggcgataaa atggagtggg tggcaccgca catgggcacc
    34801 gatgttgcgc tgatgctggg gatcgcccat acgctggtgg aaaatggttg gcacgacgaa
    34861 gcgtttctga cgcgttgcac cacaggttat gccgtcttcg cctcttattt gctgggcgag
    34921 agtgacggaa tagcgaaaaa cgccgaatgg gcggcagaga tttgtggggt taacgcagcg
    34981 aaaatccgcg agctggcggc tcttttccac caaaacacga ccatgctgat ggctggctgg
    35041 ggaatgcagc gccaacagtt tggtgagcaa aaacactgga tgatcgtcac gctggcggca
    35101 atgttggggc aaatcggcac acccggcggc ggttttggtc tttcttatca ttttgccaat
    35161 ggtggtaacc ccacgcggcg ttctgcggtg ctctcttcca tgcagggcag cttgccgggt
    35221 ggctgcgatg cggtggataa aatccctgtt gcccgcattg ttgaagcact ggaaaaccct
    35281 ggtggcgcat atcaacacaa cggtatgaac cgacatttcc cggatattcg ttttatctgg
    35341 tgggcgggcg gtgccaactt tactcatcat caggatacca atcgtctgat ccgtgcctgg
    35401 caaaaaccgg agctggtggt gatctctgaa tgcttctgga cggcggcggc gaaacacgcg
    35461 gatatcgttc tgcctgcgac tacctctttt gagcgtaatg atctcaccat gaccggtgat
    35521 tacagtaatc agcatctggt gccgatgaag caagtggtgc cgccacgcta tgaagcgcgt
    35581 aatgattttg atgtttttgc cgagttgagt gaacgttggg agaagggcgg ctatgcgcgg
    35641 tttacggaag gaaaaagtca gctgcaatgg ctggaaacgt tttataacgt tgcccggcag
    35701 cgcggggcaa gccagcaggt tgaattgccg ccatttgctg agttctggca agccaaccag
    35761 ttaattgaga tgccggaaaa cccggacagc gagcggttta ttcgcttcgc tgatttttgc
    35821 cgcgatccgc tggcgcatcc gttaaaaacc gccagcggca agattgaaat cttctcacag
    35881 cgtattgccg attacggtta cccggattgc cctgggcatc caatgtggct ggagccggac
    35941 gaatggcagg gcaatgccga accagaacag ttgcaggtac tttctgccca tccggcgcac
    36001 cgcctgcaca gccagctgaa ttacagttct ctgcgcgaat tgtacgcggt agcaaaccgt
    36061 gagcctgtca ccattcatcc tgacgatgct caggcgcgcg gcataaccga aggggatatg
    36121 gtgcgggtgt ggaactcccg tgggcagatc cttgccggag cggtcattag cgagggaatt
    36181 aaacctggcg tgatttgcat tcacgaaggg gcatggccgg atctggattt aaccgctgac
    36241 ggtatttgta aaaacggcgc agtgaacgtg ctgaccaaag atctccccag ctcgcggctg
    36301 gggaatggct gtgcgggtaa tacggcgctg gcatggctgg aaaaatacaa cggtccggaa
    36361 ctgacactta cagcgtttga accaccggcc agctcataat ccaggtgggt agttgggttt
    36421 catcctgcca tgcgcaatcg acaatgtgaa aaccctgcgc acggtaaaaa tctatcgccg
    36481 gttgattttt ttgataaacc tccagcatca gatggggata gcgctgctgc acatactgca
    36541 tcagcgcctt accaataccg cgtctgacgg ccttcggtgc gacaaacatt gctgccagaa
    36601 atcggccttc cataatgctg acaaaaccga gaagcttacc gtcctcttcc cagacccagt
    36661 tttgcgcgtt ggcaagatag gcatcccgca ccagcggaat gcagtcacgc cagtaattcg
    36721 cttttataaa gggatgcccc caggttgtac tttccagcca cagttcgagg atcgcgggga
    36781 gttctgaacg ttgcgcttcc cgaatcatgg tttatttccc ggatagcaac agcagccaac
    36841 cacatgatca ttcaccagcc cacatgcctg cataaaggag taacagattg tggtgccgac
    36901 aaacttaaaa ccacgttttt tcagtgcctt agatagggcg tcggaggcgg acgtagatgt
    36961 gggaatttcg ctcaacgttg tggcttgtgt cacctgtggc tgatgattta caaacgacca
    37021 gacaaagtcg gcaaacggtt cgccgttctg ttccatttgc aggtacgccc gcgcattacc
    37081 aataattgcc tgaattttcc ctcgatggcg gataatcccg gcgtcctgta ccagtctttc
    37141 gacatcctct tcctgcattg ctgcgacctt caccggatcg aactgatgaa agcaggcgcg
    37201 atagttttcg cgttttttga ggacggtgat ccacgataac ccagcctgct gcccttcaag
    37261 gcagatcatt tcgaacagtt ttttactgtc agtttcaggc acgccccatt cattatcatg
    37321 gtaggcaata taaagcgggt cctgactcac ccagccgcaa cgttccatac tttccctcgc
    37381 tatgtacccg atcgtaaaaa tttcagccgc ttttcttgac gcatcggtga aaaagtcaat
    37441 atttaataca gggctactat gcccgatatc cttcatgcaa tgacaataat attgccgaat
    37501 tcggctcttc cctggagtcg tgttaatgat cataaaaaaa agcggtggtc gctggcagct
    37561 aagcctgctg gcgagcgtgg taatcagtgc cttttttctc aacacagctt acgcctggca
    37621 acaagaatat atcgttgata cgcaacccgg acattccaca gagcgttaca cctgggatag
    37681 tgatcatcaa cctgattaca acgatatttt gtcgcaacgt attcaaagta gccaaagggc
    37741 gctgggactg gaagtcaatc tggcggaaga aactcctgtg gatgtgacca gcagtatgag
    37801 tatgggctgg aattttcctt tatatgaaca ggttacaacc ggcccggtcg cggcattaca
    37861 ttacgatggc acaaccacct cgatgtataa cgagtttggc gacagtacta ccacgctgac
    37921 cgatccgtta tggcatgcca gcgtgagtag cttaggctgg cgtgttgact cccggcttgg
    37981 cgatctccga ccctgggcgc aaatcagcta taaccagcaa tttggcgaga atatctggaa
    38041 ggcgcaatca ggcctgagcc ggatgacggc gacaaaccag aacggcaact ggctggatgt
    38101 caccgtaggc gctgatatgt tgctcaatca aaatattgcc gcctatgccg cgctaactca
    38161 ggcagaaaat accactaata atagcgacta tctgtatacg atgggggtta gcgccagatt
    38221 ttaacgtaac agtcacaatt gaaaccattg aataacaaca gttgtggcga tagtgggtgc
    38281 taacttacca aataataaat ttggtgaata attgtcgcgt cattcattcc tgaactaagg
    38341 catttcattc cgttctgatg gcatttcatg ccgtttttcc ccaggcataa aatgcacttc
    38401 gttatggttg tcggcagaga tttttccttt ttattactgc aggaatactg ccatgacacc
    38461 ttcaaattat cagcgtaccc gctggctgac actcatcggt actatcatta cccagtttgc
    38521 actggggtcg gtttatacct ggagcctgtt taatggcgcg ctttctgcca agctggatgc
    38581 gccggtaagc caggtcgctt tctctttcgg cttgttaagt ctggggctgg caatttcgtc
    38641 ttctgttgcg ggcaaattgc aggaacgttt tggcgttaaa cgcgtcacca tggcttccgg
    38701 cattttgctg ggattaggct tcttcctgac cgcgcattcc aacaacctga tgatgctgtg
    38761 gttaagcgcc ggtgtgctgg tcggtctggc ggatggcgcg ggttacctgc tgacgctctc
    38821 taactgcgtg aagtggttcc cggagcgtaa aggtctgata tccgcgttcg ctatcggttc
    38881 ttatggtctg ggtagcctgg gtttcaaatt tatcgacacg cagctgctgg aaacggtggg
    38941 tctggaaaaa acctttgtga tttggggagc gattgcgctg gtgatgattg ttttcggcgc
    39001 aacgttaatg aaagacgcac caaaacagga agtgaaaacc agcaatggtg tggtggagaa
    39061 agattacacg ctggcagagt cgatgcgtaa accgcagtac tggatgttag cggtaatgtt
    39121 cctgaccgcc tgcatgagcg gcctgtacgt gattggggta gcgaaagata tcgcccaaag
    39181 tctggcacac cttgatgtgg tttccgcagc caatgcagtc actgttattt ccatcgccaa
    39241 cctttcaggt cgtctggtgc tgggtattct gtctgacaaa atcgcccgta tccgtgttat
    39301 caccattggt caggtgatat cgctggtggg tatggcggcc ctgctgtttg caccattgaa
    39361 tgcagtgacg ttctttgcag cgattgcctg cgtggcattt aactttggcg gcactattac
    39421 cgtctttccg tcactggtca gtgagttctt tggcctcaat aacctggcga aaaactacgg
    39481 tgtgatttac ctcggtttcg gtatcggtag catttgtggt tcgattatcg cctcactgtt
    39541 tggcggcttc tatgtgacct tctacgtgat tttcgccctg ctgattctgt cattggcgct
    39601 ttctacgaca attcgtcagc cagagcagaa agtattgcgt gaggcgcata tttagaaata
    39661 tgttgctact gaaataaatg cgaggaggga gacttcaaag tctccctttt tttatggacg
    39721 cat
//
LOCUS       DAZFWL020000029        39392 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFWL020000029 DAZFWL020000000
VERSION     DAZFWL020000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.029"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(93..1196)
                     /locus_tag="ACHKNP_RS08190"
                     /old_locus_tag="pgaptmp_001638"
                     /pseudo
     CDS             complement(93..1196)
                     /locus_tag="ACHKNP_RS08190"
                     /old_locus_tag="pgaptmp_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(1563..2174)
                     /locus_tag="ACHKNP_RS08195"
                     /old_locus_tag="pgaptmp_001639"
     CDS             complement(1563..2174)
                     /locus_tag="ACHKNP_RS08195"
                     /old_locus_tag="pgaptmp_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4703830.1"
                     /translation="MDKEEKTVSSAERFIDMEGINRFSERLAMAMGNMTNVELATLCG
                     LSEATVRNYRKGKNFPPLDKLALIAKACNCSLEWLATGKNEDECGKIENNCDSGSGID
                     QALSSLSPQEKQLILTFIRREGTNNLVRLAAMNTSTISQDAVESIIDALPLRPVLKNA
                     IKIGMAGSEETDKEILRVIEKYNSSNNATQESGAVTVKKNVVG"
     gene            2331..2597
                     /locus_tag="ACHKNP_RS08200"
                     /old_locus_tag="pgaptmp_001640"
     CDS             2331..2597
                     /locus_tag="ACHKNP_RS08200"
                     /old_locus_tag="pgaptmp_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4703831.1"
                     /translation="MRVSRQASNNLFVREGCDWSAKAVYAALENKGTDLPELERELGL
                     KSGSMRNVFYRQCRKYEAAIAEKIGVEPAVIWPSRYQVDAREVA"
     gene            2608..4698
                     /locus_tag="ACHKNP_RS08205"
                     /old_locus_tag="pgaptmp_001641"
     CDS             2608..4698
                     /locus_tag="ACHKNP_RS08205"
                     /old_locus_tag="pgaptmp_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HGU4703832.1"
                     /translation="MWVTAKECVGLPDLPMMEHNIRHRLTKAAAGNAAMLRKRKGTKA
                     IEFNIDCLPSGAQQVLRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVMKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVAVQQPAVDDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            4770..5702
                     /locus_tag="ACHKNP_RS08210"
                     /old_locus_tag="pgaptmp_001642"
     CDS             4770..5702
                     /locus_tag="ACHKNP_RS08210"
                     /old_locus_tag="pgaptmp_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4703833.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRTFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            5705..5926
                     /locus_tag="ACHKNP_RS08215"
                     /old_locus_tag="pgaptmp_001643"
     CDS             5705..5926
                     /locus_tag="ACHKNP_RS08215"
                     /old_locus_tag="pgaptmp_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703834.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVS"
     gene            5939..6193
                     /locus_tag="ACHKNP_RS08220"
                     /old_locus_tag="pgaptmp_001644"
     CDS             5939..6193
                     /locus_tag="ACHKNP_RS08220"
                     /old_locus_tag="pgaptmp_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703835.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            6195..6476
                     /locus_tag="ACHKNP_RS08225"
                     /old_locus_tag="pgaptmp_001645"
     CDS             6195..6476
                     /locus_tag="ACHKNP_RS08225"
                     /old_locus_tag="pgaptmp_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HGU4703836.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            6473..6745
                     /locus_tag="ACHKNP_RS08230"
                     /old_locus_tag="pgaptmp_001646"
     CDS             6473..6745
                     /locus_tag="ACHKNP_RS08230"
                     /old_locus_tag="pgaptmp_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703837.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKASALMKDMSMRSGVIKGNEFNQQG"
     gene            6750..7043
                     /locus_tag="ACHKNP_RS08235"
                     /old_locus_tag="pgaptmp_001647"
     CDS             6750..7043
                     /locus_tag="ACHKNP_RS08235"
                     /old_locus_tag="pgaptmp_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703838.1"
                     /translation="MSKVRVIFEFKHVSHDEKPAGNDCVEVHEKIGVDVKTERDTNNR
                     PTSLCDVYASILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            7055..7585
                     /locus_tag="ACHKNP_RS08240"
                     /old_locus_tag="pgaptmp_001648"
     CDS             7055..7585
                     /locus_tag="ACHKNP_RS08240"
                     /old_locus_tag="pgaptmp_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4703839.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            7683..8225
                     /locus_tag="ACHKNP_RS08245"
                     /old_locus_tag="pgaptmp_001649"
     CDS             7683..8225
                     /locus_tag="ACHKNP_RS08245"
                     /old_locus_tag="pgaptmp_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703840.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDRISEFYFSGALPAGWRKRDDVAFIGDGPCFIARPDELCPEGPAIAAMVEA
                     AERDLRKHPDFLVWLCEKLGVMRIPSMFNTDPWWTPSFSRDALCVVFKVGAYGREING
                     CIPEECQEIKHSEYVALTEE"
     gene            8229..8762
                     /locus_tag="ACHKNP_RS08250"
                     /old_locus_tag="pgaptmp_001650"
     CDS             8229..8762
                     /locus_tag="ACHKNP_RS08250"
                     /old_locus_tag="pgaptmp_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703841.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGVLAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQESAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITEYVLRY
                     LIVDRTAAPATISAEVA"
     gene            8762..9277
                     /locus_tag="ACHKNP_RS08255"
                     /old_locus_tag="pgaptmp_001651"
     CDS             8762..9277
                     /locus_tag="ACHKNP_RS08255"
                     /old_locus_tag="pgaptmp_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703842.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVSCRSVFE
                     FSISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILFGPVEWFCVDDVIYLECGVKPAKQKMTEILSD
                     EFYAAEKRVRG"
     gene            9281..9832
                     /locus_tag="ACHKNP_RS08260"
                     /old_locus_tag="pgaptmp_001652"
     CDS             9281..9832
                     /locus_tag="ACHKNP_RS08260"
                     /old_locus_tag="pgaptmp_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HGU4703843.1"
                     /translation="MMILQPMGRKGRAPAHVRAWTPEEDALLIALYPSTPVKDIAVRI
                     KRSFWGVHNRIVLLRGTYPELLKCKRLRFKPDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVRLIRELRDAESPRRLTFREIGEKFELSE
                     GTVSFIYHRRRTAEDVVLRELMP"
     gene            9829..10140
                     /locus_tag="ACHKNP_RS08265"
                     /old_locus_tag="pgaptmp_001653"
     CDS             9829..10140
                     /locus_tag="ACHKNP_RS08265"
                     /old_locus_tag="pgaptmp_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703844.1"
                     /translation="MITTLFVESDEPLVCAVGMPVCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHISALP
                     E"
     gene            10155..10505
                     /locus_tag="ACHKNP_RS08270"
                     /old_locus_tag="pgaptmp_001654"
     CDS             10155..10505
                     /locus_tag="ACHKNP_RS08270"
                     /old_locus_tag="pgaptmp_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703845.1"
                     /translation="MRSYIDNEKLKTISDCLSLLAKIKETIEEIKFQLEYEPCGDDTW
                     RNSARKALAVFQKQRRTVEYRLAVLRQEEKERNIRRHELVNDFLVRELKERVPESVFF
                     ECEAVARSKALETD"
     gene            10521..10853
                     /locus_tag="ACHKNP_RS08275"
                     /old_locus_tag="pgaptmp_001655"
     CDS             10521..10853
                     /locus_tag="ACHKNP_RS08275"
                     /old_locus_tag="pgaptmp_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703846.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKIVSFMDKECDKRDMPEI
                     IMDDMILHSAVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            10846..11043
                     /locus_tag="ACHKNP_RS08280"
                     /old_locus_tag="pgaptmp_001656"
                     /pseudo
     CDS             10846..11043
                     /locus_tag="ACHKNP_RS08280"
                     /old_locus_tag="pgaptmp_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11033..11329
                     /locus_tag="ACHKNP_RS08285"
                     /old_locus_tag="pgaptmp_001657"
     CDS             11033..11329
                     /locus_tag="ACHKNP_RS08285"
                     /old_locus_tag="pgaptmp_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001509933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703847.1"
                     /translation="MDSSSQLFWYLYITSFFGAFVITRWLCRKVISIFHKKYLIGQAA
                     TSLINALARYHKGECYSRIKTDDGFNIFVIPPEYRVQLDRENNTYHIRRSVDTA"
     gene            11326..11835
                     /locus_tag="ACHKNP_RS08290"
                     /old_locus_tag="pgaptmp_001658"
     CDS             11326..11835
                     /locus_tag="ACHKNP_RS08290"
                     /old_locus_tag="pgaptmp_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HGU4703848.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSVLLAVTGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQNPVNRALKPGTVTAKIRAIWKVMHRQGFITDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            11905..12330
                     /locus_tag="ACHKNP_RS08295"
                     /old_locus_tag="pgaptmp_001659"
     CDS             11905..12330
                     /locus_tag="ACHKNP_RS08295"
                     /old_locus_tag="pgaptmp_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HGU4703849.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            12402..12902
                     /locus_tag="ACHKNP_RS08300"
                     /old_locus_tag="pgaptmp_001660"
     CDS             12402..12902
                     /locus_tag="ACHKNP_RS08300"
                     /old_locus_tag="pgaptmp_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4703850.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            12937..13365
                     /locus_tag="ACHKNP_RS08305"
                     /old_locus_tag="pgaptmp_001661"
     CDS             12937..13365
                     /locus_tag="ACHKNP_RS08305"
                     /old_locus_tag="pgaptmp_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703851.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASAPVPDDVADRVRRAVCEIRACEAGADPR"
     gene            13349..13567
                     /locus_tag="ACHKNP_RS08310"
                     /old_locus_tag="pgaptmp_001662"
     CDS             13349..13567
                     /locus_tag="ACHKNP_RS08310"
                     /old_locus_tag="pgaptmp_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703852.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            13578..13805
                     /locus_tag="ACHKNP_RS08315"
                     /old_locus_tag="pgaptmp_001663"
     CDS             13578..13805
                     /locus_tag="ACHKNP_RS08315"
                     /old_locus_tag="pgaptmp_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU4703853.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            13786..14094
                     /locus_tag="ACHKNP_RS08320"
                     /old_locus_tag="pgaptmp_001664"
     CDS             13786..14094
                     /locus_tag="ACHKNP_RS08320"
                     /old_locus_tag="pgaptmp_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HGU4703854.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            14091..14381
                     /locus_tag="ACHKNP_RS08325"
                     /old_locus_tag="pgaptmp_001665"
     CDS             14091..14381
                     /locus_tag="ACHKNP_RS08325"
                     /old_locus_tag="pgaptmp_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HGU4703855.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRASVPGVKKPRARG"
     gene            14384..14965
                     /locus_tag="ACHKNP_RS08330"
                     /old_locus_tag="pgaptmp_001666"
     CDS             14384..14965
                     /locus_tag="ACHKNP_RS08330"
                     /old_locus_tag="pgaptmp_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HGU4703856.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            14965..16629
                     /locus_tag="ACHKNP_RS08335"
                     /old_locus_tag="pgaptmp_001667"
     CDS             14965..16629
                     /locus_tag="ACHKNP_RS08335"
                     /old_locus_tag="pgaptmp_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703857.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMLRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAIEGGYRDDDEAYHEFDRGCW"
     gene            16629..18218
                     /locus_tag="ACHKNP_RS08340"
                     /old_locus_tag="pgaptmp_001668"
     CDS             16629..18218
                     /locus_tag="ACHKNP_RS08340"
                     /old_locus_tag="pgaptmp_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HGU4703858.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAVLDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVREQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIEAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            18202..19527
                     /locus_tag="ACHKNP_RS08345"
                     /old_locus_tag="pgaptmp_001669"
     CDS             18202..19527
                     /locus_tag="ACHKNP_RS08345"
                     /old_locus_tag="pgaptmp_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HGU4703859.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKVARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGQVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSVKHQNDGIALTPEDFAQLPAMLAAPDAVLWDHVHQNLLYITETRDG
                     TAKIAVNAPYGVKRQPDKLDVVINAYRVNKFDIEKAIEGGKLELLEGKL"
     gene            complement(19527..19607)
                     /locus_tag="ACHKNP_RS08350"
                     /old_locus_tag="pgaptmp_001670"
     tRNA            complement(19527..19607)
                     /locus_tag="ACHKNP_RS08350"
                     /old_locus_tag="pgaptmp_001670"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            19646..20119
                     /locus_tag="ACHKNP_RS08355"
                     /old_locus_tag="pgaptmp_001671"
     CDS             19646..20119
                     /locus_tag="ACHKNP_RS08355"
                     /old_locus_tag="pgaptmp_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HGU4703860.1"
                     /translation="MSSIDAAVVVDVARLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYSAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            20296..21420
                     /locus_tag="ACHKNP_RS08360"
                     /old_locus_tag="pgaptmp_001672"
     CDS             20296..21420
                     /locus_tag="ACHKNP_RS08360"
                     /old_locus_tag="pgaptmp_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HGU4703861.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIKGHQAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDENTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTSLQTD
                     TVTVPDKKTATAALSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            21420..22367
                     /locus_tag="ACHKNP_RS08365"
                     /old_locus_tag="pgaptmp_001673"
     CDS             21420..22367
                     /locus_tag="ACHKNP_RS08365"
                     /old_locus_tag="pgaptmp_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HGU4703862.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAVQPDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            22411..22764
                     /locus_tag="ACHKNP_RS08370"
                     /old_locus_tag="pgaptmp_001674"
     CDS             22411..22764
                     /locus_tag="ACHKNP_RS08370"
                     /old_locus_tag="pgaptmp_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HGU4703863.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVSPEQLARLEAEPCLTVRILQTPADDAGSVAGVVHAVAGTDLAEAGQDTPR
                     KKTGNKAKQAKKARA"
     gene            22761..23180
                     /locus_tag="ACHKNP_RS08375"
                     /old_locus_tag="pgaptmp_001675"
     CDS             22761..23180
                     /locus_tag="ACHKNP_RS08375"
                     /old_locus_tag="pgaptmp_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HGU4703864.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            23177..23737
                     /locus_tag="ACHKNP_RS08380"
                     /old_locus_tag="pgaptmp_001676"
     CDS             23177..23737
                     /locus_tag="ACHKNP_RS08380"
                     /old_locus_tag="pgaptmp_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HGU4703865.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            23738..23983
                     /locus_tag="ACHKNP_RS08385"
                     /old_locus_tag="pgaptmp_001677"
     CDS             23738..23983
                     /locus_tag="ACHKNP_RS08385"
                     /old_locus_tag="pgaptmp_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HGU4703866.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRLLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKSAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            23980..25479
                     /locus_tag="ACHKNP_RS08390"
                     /old_locus_tag="pgaptmp_001678"
     CDS             23980..25479
                     /locus_tag="ACHKNP_RS08390"
                     /old_locus_tag="pgaptmp_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGU4703867.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATEFWGRGSMIALMVAEFIAINPDAELYAIAQGSGT
                     GQAQACLMNIIGTASQDGVLHIYIGGRRYLLAVTKGQKGKELIDALVSVINADADAPF
                     SAYAGQVADNASGLKGSMGINARFTGECSVHDIRVNYHDGETTPGGLTIELGYPKTKA
                     TNPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWMAHTGT
                     FGEVQAFGAKRNDFLATCTFIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALPS
                     RMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLDV
                     ETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWVE
                     LGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            25488..25853
                     /locus_tag="ACHKNP_RS08395"
                     /old_locus_tag="pgaptmp_001679"
     CDS             25488..25853
                     /locus_tag="ACHKNP_RS08395"
                     /old_locus_tag="pgaptmp_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGU4703868.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASATSTRVQ"
     gene            25868..26344
                     /locus_tag="ACHKNP_RS08400"
                     /old_locus_tag="pgaptmp_001680"
     CDS             25868..26344
                     /locus_tag="ACHKNP_RS08400"
                     /old_locus_tag="pgaptmp_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HGU4703869.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            26471..28546
                     /locus_tag="ACHKNP_RS08405"
                     /old_locus_tag="pgaptmp_001681"
     CDS             26471..28546
                     /locus_tag="ACHKNP_RS08405"
                     /old_locus_tag="pgaptmp_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4703870.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSQAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGMGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPPSPSSGGAGFWGRMMGRAGGLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGAL
                     WAGAMAAPVLLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNY
                     LGGWLTEAWQKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNV
                     WNGGNYGLW"
     gene            28533..29882
                     /locus_tag="ACHKNP_RS08410"
                     /old_locus_tag="pgaptmp_001682"
     CDS             28533..29882
                     /locus_tag="ACHKNP_RS08410"
                     /old_locus_tag="pgaptmp_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HGU4703871.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGQVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            29866..30990
                     /locus_tag="ACHKNP_RS08415"
                     /old_locus_tag="pgaptmp_001683"
     CDS             29866..30990
                     /locus_tag="ACHKNP_RS08415"
                     /old_locus_tag="pgaptmp_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HGU4703872.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            30980..31594
                     /locus_tag="ACHKNP_RS08420"
                     /old_locus_tag="pgaptmp_001684"
     CDS             30980..31594
                     /locus_tag="ACHKNP_RS08420"
                     /old_locus_tag="pgaptmp_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU4703873.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNITVDKNLHVKGNV
                     SIDGTGRSQGAFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            31587..32024
                     /locus_tag="ACHKNP_RS08425"
                     /old_locus_tag="pgaptmp_001685"
     CDS             31587..32024
                     /locus_tag="ACHKNP_RS08425"
                     /old_locus_tag="pgaptmp_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HGU4703874.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPIGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GVATSVVCHAMRVGHDRLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            32024..33106
                     /locus_tag="ACHKNP_RS08430"
                     /old_locus_tag="pgaptmp_001686"
     CDS             32024..33106
                     /locus_tag="ACHKNP_RS08430"
                     /old_locus_tag="pgaptmp_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGU4703875.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            33097..33657
                     /locus_tag="ACHKNP_RS08435"
                     /old_locus_tag="pgaptmp_001687"
     CDS             33097..33657
                     /locus_tag="ACHKNP_RS08435"
                     /old_locus_tag="pgaptmp_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HGU4703876.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEENI"
     gene            33657..34568
                     /locus_tag="ACHKNP_RS08440"
                     /old_locus_tag="pgaptmp_001688"
     CDS             33657..34568
                     /locus_tag="ACHKNP_RS08440"
                     /old_locus_tag="pgaptmp_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4703877.1"
                     /translation="MFHVDNNSGVANMPELAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENAETARNNLDVYSRSEGDNRYLRR
                     DQNGADIQDKGTFIDNLGLRETVNKAADALPSAGTAVAANRLATPRNINGVPFDGTLD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEMSWNYSAPSGCMLSGIDVQETGSR
                     SADNIGGVYYRPVQIYINNAWRTVSSV"
     gene            complement(34603..35124)
                     /locus_tag="ACHKNP_RS08445"
                     /old_locus_tag="pgaptmp_001689"
     CDS             complement(34603..35124)
                     /locus_tag="ACHKNP_RS08445"
                     /old_locus_tag="pgaptmp_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4703878.1"
                     /translation="MQLINVKRYYPKDKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            35481..35666
                     /locus_tag="ACHKNP_RS08450"
                     /old_locus_tag="pgaptmp_001690"
     CDS             35481..35666
                     /locus_tag="ACHKNP_RS08450"
                     /old_locus_tag="pgaptmp_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703879.1"
                     /translation="MFISCVPSKATPLILQAFASLLAATAVPFDGRAFAALFTVSRKP
                     RFQGCRISALKGFSTSF"
     gene            35680..36240
                     /locus_tag="ACHKNP_RS08455"
                     /old_locus_tag="pgaptmp_001691"
     CDS             35680..36240
                     /locus_tag="ACHKNP_RS08455"
                     /old_locus_tag="pgaptmp_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4703880.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            36340..38379
                     /locus_tag="ACHKNP_RS08460"
                     /old_locus_tag="pgaptmp_001692"
     CDS             36340..38379
                     /locus_tag="ACHKNP_RS08460"
                     /old_locus_tag="pgaptmp_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4703881.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPRIMQLARRSTVTPGGKA
                     CQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLHIAKKLLPFIPADAGIL
                     LVPCCRGGSAFTVGADGTYSDSTGASEDSARWGVDKPLYKDLISRTKAALAKNPKNRL
                     LAVVWMQGEFDIDAKPTEHSALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTY
                     FWKQKNEPAYQAIYGGYKNKTDKNIHFVPLMTDENGVNVPTNNPAEDPDIESIGYYGS
                     TWRNSAATWTSADRASHFSSWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAPVP
                     SPSETEAVTTTLLSYRVSESEGNLKAQGWEPAGGKAEIISDAGGTGGKAMKLTKETGK
                     SSWYLDHDAGTGSELLKNGGLISCRFKVPGDLVANQYVMALYWPVSSLPQGVTLTGDA
                     GNNLLASFYIQTDAKDLNVMYHNAKVATNNQKLGTFGAFDNEWHTLAFRFAGNNSLQV
                     IPVIDGQDGAAFTLTQSPVGTFPVDKLRVTDITKNATYPVLIDSIVVEVKKAVTE"
     gene            38526..38708
                     /locus_tag="ACHKNP_RS08465"
                     /old_locus_tag="pgaptmp_001693"
     CDS             38526..38708
                     /locus_tag="ACHKNP_RS08465"
                     /old_locus_tag="pgaptmp_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4703882.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPTPL"
     gene            38744..38989
                     /locus_tag="ACHKNP_RS08470"
                     /old_locus_tag="pgaptmp_001694"
     CDS             38744..38989
                     /locus_tag="ACHKNP_RS08470"
                     /old_locus_tag="pgaptmp_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4703883.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENKPQPVEQPEPVQPGTDGAMM"
     gene            complement(39028..39392)
                     /locus_tag="ACHKNP_RS08475"
                     /old_locus_tag="pgaptmp_001695"
                     /pseudo
     CDS             complement(39028..39392)
                     /locus_tag="ACHKNP_RS08475"
                     /old_locus_tag="pgaptmp_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         9444 a         9822 c        11209 g         8917 t
ORIGIN      
        1 aacttcttgt ctagctggct ctatgtccac tggagaacaa ccacacaata aaaataaaac
       61 taaaaatata tattttttca tatttcctcc tgttaattga cccagcattc ttgccacgtt
      121 ctacaagcca gagtcataac tgaagcgtca acacctgcac cgataccaac agattctgtc
      181 gatgattctg gattatcaac atccactaca taagtatacg caaaatgccc cccaactccg
      241 ccgccaaccg ttacacctac tgaattgctg actcccgatg caggtaaaga cttggtctca
      301 gaatatgtga tagccgcatt tatcccacca ccaattcctg catgtccgca tgctgtagca
      361 taaatacaaa tatcagataa catttcacct ttctttttag caaacgcaac gccaagtcct
      421 gcatgaccac ctaatgatat tagatctcca ctgccagtaa tatcaattga tgatgttcca
      481 tatgaatcat catgccaaac atcatataat aattgatata aattatacaa acccaaagga
      541 tccatcctta ttatcggatt cagtgggtaa ttatacaggt tccatccccc cgccagccca
      601 atggggtctt gtgtaatata cctcccctgt agcgggtcat agtaccggtt ccggttatag
      661 tacagccctg attcctcatc atgctgctgc cccggcagac ggtatggctg gtgcaggtaa
      721 tacgggttct cttcattaag ctggttgccc cattcatcat actccccgcg ccacgccgta
      781 ttgccgtctt cgctgatgag cgccagcggc aggccccggt ggtcgcagtg gtaaaaatga
      841 acttttcgcg ccggtgtgta ttccggctcc acctgtctgg ccagttgctc caccgtcagc
      901 ccgcactgcg caagccacgc ccggctttca ctgctcacgc ggtctgcccg gatttcttcc
      961 tccagcctgt ccagcagccg caccagttca gccggaaata ccacgccgtg cccgtcctcg
     1021 ctcccttcct gctgcagctt ctccgccagg ctgcggcgct gcgctttctc ccgctcgccg
     1081 ttgtctgttt cgatgcggat gagcggcgcg aagctccccg gctgatatac cgtctggata
     1141 cgcgtggtgt cggtctgtac cgtcgtcagc cggtcgccgt cccacccgta ccacgtgtaa
     1201 ccaggtgaat catttgatac cgacaagggt tttagtgaaa ggaggtgaaa tcacctgtaa
     1261 gatgctgaaa taaaacaggt gatgtatact tcggacggaa aactcttttt gataccgact
     1321 gctcaacaaa tagtcaaaag atcaaaaaac ggcgacacat ttgatactgc taatttaccc
     1381 ttaaaatctt cttcaaaaca tcttcaaaac atcttcaaac tgccaaaatg ctcacataat
     1441 cgatctaact ataatcagta tgataatcat catgaattac tattcaatag cgtgattatc
     1501 ttgggatgag tcatatgatc tctatatgta tttacagcac cacctcatga tagcgatggc
     1561 gcttaaccaa caacattttt ctttactgtc actgcccctg attcctgggt ggcgttattg
     1621 ctcgagttgt acttctcaat aacgcgcaaa atctctttgt ctgtttcttc acttcccgcc
     1681 attcctatct ttattgcatt ttttagtacg ggacgtaatg gcaaggcatc aatgatgctt
     1741 tctaccgcat cctggctgat tgtagatgta ttcattgctg ccagcctgac aaggttgtta
     1801 gtaccttctc gcctgatgaa tgtaaggatt aattgcttct cttgggggct tagagagcta
     1861 agcgcctgat caatacccga acctgaatcg caattgtttt cgatttttcc gcattcatct
     1921 tcgttcttgc ctgtggccaa ccattcgagt gaacaattac aggctttagc tattaaggcc
     1981 agtttatcca aaggagggaa gtttttcccc ttcctgtaat ttcttactgt ggcttcagat
     2041 agtccacaaa gggtcgccag ttcgacgttt gtcatgtttc ccatagccat agctaagcgt
     2101 tctgaaaatc ggtttattcc ttccatatca ataaaccgtt ccgcactgga tacggttttt
     2161 tcttccttat ccatataacc ccctataaaa caaagaaatt atcgacatcg catttaattt
     2221 cgatatgctc gcaaaaaacc gttgatatcg aattattttg cgattatgat ttacctaaat
     2281 cgataaccgg ttttgattat cggttcaggt aaacatgaga ggatcacaca atgagggtat
     2341 ccagacaagc atcgaataat ttgtttgttc gagaggggtg tgactggtct gctaaagcgg
     2401 tatatgcagc ccttgaaaac aaaggcacag acctccctga actggaaagg gagttaggat
     2461 taaaaagcgg atcaatgcgc aatgtgtttt accgccagtg ccgtaaatac gaagccgcca
     2521 tcgccgaaaa aataggggtt gaacctgctg taatctggcc cagcagatac caggtggacg
     2581 ccagagaagt ggcttgatgg gggatgtatg tgggtaacag caaaagaatg tgttggtctt
     2641 cctgatttac caatgatgga acataacatt cgccatcgac tcacaaaagc agctgcggga
     2701 aatgcagcca tgttacgcaa acgtaagggg acaaaagcaa tcgaatttaa tatcgattgt
     2761 ttgccttctg gcgcgcaaca agtattgcgt gaacgttatg cccttcaact gatgacgcaa
     2821 aaagctgatg aatcaccggc tccggtggtg acaaaggcca gacgctcgcc tgccgtggtt
     2881 gatgcggtgg aggcatatcg cggatcacca caactgatgg ttgaacgcct taatgccctg
     2941 actgaaaacc agcgccaggt ggctgatgca cgaattgcga tcgtcagcga agtgatgaaa
     3001 gtcgcgcaac aacccggttt cagctgtgcg aaagctatcc ggtttatcgt tgacaacctg
     3061 gcacgttcgc aactggacga gcgcattgtg gcaatggttg agacggcgaa cgccaaaaag
     3121 ggaaacagcc gtgcgttgag tgaaataacg ctgaaacgct ggattgcggc ctttaacaag
     3181 gcgcagaacg ccgctgaacg cctgctttta ctggcaccgg gtaagcgcca ggaaataaaa
     3241 gccgaagata ttaactggct gcccgaattt ctggcgcagt atcgccagtc aaacggccga
     3301 ccaatgaccg aggcttacga ggattttgtc gctgaatggc agcaccggca cgctgatgag
     3361 ccttatatgc tcgatatcat gccgtcttat gacaccattc gccgcgcaat gaagaaactg
     3421 ccggaagtgg tgaaacaaaa aggccgggtg accggcagtg aataccgcca gcttgaggga
     3481 ttcacgcgcc gcgactggtc cagaatgccg gtgaattatg tctggattgg tgacggtcac
     3541 ggcatgaagc tgaaatgcgc acacccggtt cacggtcggc catttgcacc ggaagtgacc
     3601 tttgttatcg acggtggcac gcgctttgtg gtgggctgga gccttgacct ggctgaaaat
     3661 gttttcgccg tagccggtgc catacagcac ggcattcgcc atcacggcaa accgtttctg
     3721 tattactcgg ataacggctc cggggaaacc gccgacatcc tggataagga ggttgtgggg
     3781 atactgccgc gactggggat taatcacccg accgggattg ccggtaatcc gcagggacga
     3841 ggcattatcg aacggcttaa ccgcacatta ccgatgcgca tagcccgtaa ataccgcacc
     3901 tatttcggga aaggtgcaga tcgcgagacg ttacgcaaaa ccaaccgcga tttacgctcg
     3961 gcatttactg ccctgcaaca gggcaaacgg ctgaacgccc ggcagcagtc agcgatgcgt
     4021 gatttaccgt cctggtctga actgattgat gccattcgtg acggtgttga gtggtacaac
     4081 aaccggccgc acgatgaatt acccatgaag ccgaacggga aacattacag cccggcggag
     4141 ttcagaaaaa aacgcctggc ggaagaggac acggaaattg aatggctgtc cgatgttgag
     4201 ctgcgggata tgttccggcc gatggtggaa cgccctgtaa gacgctgtga aatacgctgg
     4261 ctgaataata tttactacgc gcccgagctg cgtgacgagc atggccgtaa ggtgcttatc
     4321 agctatgaca ttcatgatgc cgaacgaatt accgtacgtc gcctggatgg cagcgtgatt
     4381 tgcgaggcgg tatgggacgg caataaacgc gaagccttcc cggtcagcgc ggaatactac
     4441 aaacagcagc aacgccttaa aggcatgcgt aaacgcgcag aggaaaaaat ccgtgatgcc
     4501 gaggatgagg tggtcaacgt gctggagcac aagccgcagg agccctggct ggaaaacatc
     4561 tatcgcccgg taggtaatac ggtggccgtt cagcaacctg ccgttgatga tgagcctgat
     4621 gaagaatacg agcgtaattt ccagcgggga ttgcagttgc tcgaagcgaa attaaaagaa
     4681 aatgacccgc tggcctgaaa taaaaaaata acccgagcgg cgactcaggt tatttgatta
     4741 aacaaggtat caaatgagag gttaataata tgactgatat taacgatgta atcaagacca
     4801 ttgatgaact tattgatggc ggcgtactga cgcagtatgc catcgccaga gaggcgggaa
     4861 tttccgacgg cacattatcg gctttccgta agggaaaata taaaggcgat aacgccgctg
     4921 tggctgcttc cctgcgttcc tggtatgaga actggaataa acaaagcgca ctgccggaac
     4981 cgccgcagtt tgtggaaacg cagacagtcc aggagctgcg cgcactgttt caggcggttc
     5041 gtctgatggg ctgtattaac gtcatagtgg gcgtgcccgg ggtgggtaaa acggccaccg
     5101 cccgtaatta ctgccaggag caaccaaaca cctggatgat caccctgtca cccgcgcact
     5161 ccagcgtcac ggagtgtctg ctggagctgg ccgatgcgct ggggattgat tacacccgcg
     5221 cgaacaaagg ggcattatcc cgcgctatcc gccgtcgcct gatgggaacg cgtggactgg
     5281 tgattgtgga tgaggcggat catcttggta ttgacggtct ggagcaactc cgggcaattc
     5341 aggacgccac ggggatcggg atggtgctta ttggtaaccc gcgcggattg tttaaaggtg
     5401 gacgccgcac ctttgatgat ttatcgcgcc tgttcagccg tcttgcccgt acaaaacaac
     5461 ttcgcaaggc caaaaaggcg gatgtgctgg ccattgccag ggcgtggggg atcagtggtg
     5521 aggccgagct ggccgtcatg caggctatcg ctgaaaagcc gggagcgtta cgcgttctga
     5581 cacatacgct taaccaggcg tggctcaccg ccagcggtga aggcgcggcg ctgacagaaa
     5641 aacatattaa tgcggccttt aaagaggttt ataccaaccc tgaattactc tcacaggtgt
     5701 gattatggcg gtatttaata ttcctgatat ttacggacgc ttttacctgg ttaatttcga
     5761 taacgtgaag gtgatttcac tggccgaaaa taaagaatgt ggcgatttac tttttgaatt
     5821 taatgaccgc acacgaatgg tgatatctgc cggacttgat cgagaaggtg cgacagacgt
     5881 ttacagcgga atatgccgtt ctgttggtgc gaaacaagtc agctaaatga ggtgttatat
     5941 gaatatgcaa tcctgcggta acaaaatgaa tttattcgac tccctgaaca gcgcgcgccg
     6001 tctgaccgaa cttgccggtg cggtactgga acgcagtaag cgctatccgc aacgttttgc
     6061 actgaaaacc acgccgccgg taggcaacgt gcagggaacc ggtgaaattg aaatcaccat
     6121 acagaccaac ggcctgcgcc gccgtgtgaa ggccacccgc atcagcggct gcacggttta
     6181 ctgggaggtg tgaggtgaaa aaaaatctca ttgcatgggc gtggtcgagt ggtcttattg
     6241 agtttggtta cgtcctgccg gaaggtgcat tgccgatagt tgccggaaag cctgccacgg
     6301 tacggcatgt gattgaggtt atggcgcgtc atggacgtga tgaacaggag cagttactgg
     6361 ttccggggat accggaagcg gtgacggagg aagaagcctt taatgccatg attcggttct
     6421 gccgtgaggt cagacgccgg gtcagttatc caaacagaac gaggaccaga ggatgagtaa
     6481 agtcgtacgc attattttcg aatacaagga gcacgttatc cataaaaacg ctgatggaac
     6541 agtgcgcatg ggggtaagtc tggacatacg ttcaaccggg ataaagcaga aaggtgatgg
     6601 acccgccatg atttttgggg tggttatgct cgcggaaagc agagactttg ctgaacttgt
     6661 ggcaatgaaa gccagtgcgc tcatgaaaga tatgagcatg cgttccgggg ttattaaagg
     6721 taatgaattt aatcagcagg ggtaattcca tgagcaaagt acgcgttatt tttgaattta
     6781 agcatgtttc gcatgacgaa aaaccggcag gcaatgactg tgttgaagtg catgaaaaga
     6841 ttggtgtgga tgtaaaaaca gaacgtgata cgaataacag gccgacgtca ctctgtgacg
     6901 tttatgcaag tattctccag tatcacagtc ctgaaattat tcagtttctc tcagcggaat
     6961 ttcaggcatc tgtacaggct tttggggcgg atgccatcat taaacgccac cgcgtgcata
     7021 aagcatcagg cacactgcaa taaggaaaaa caaaatggca aaacgcgtta caaaattaaa
     7081 ggccgcagca gaggcggcac cgcagacccg tgaagaggtc agccgcgata tccgcaccct
     7141 gggcgatatt cagcgagagg cgctgcgcct ggaaacagcg atgaatgatg aagtggcaga
     7201 aatcaccgcc cgttatacgc cgcagattga aaaccttaaa aaagaaatca aagtgctttt
     7261 taaggggatt caggactggt gcaaaaccaa ccgtgatgag ctgacgaacg gcggcaaaac
     7321 caaaactgcc aatctgacca ccggaacggt gtcatggcga ctggggaatc catcatgcag
     7381 cgtcagtcgt gatgtggaag gtgtgattga aatgctgcgc cgtatgggcc ttgagcgttt
     7441 tatccgcacg aaagaggaag tgaacaagga agcggtcctg gcggagccgg atgcggtgaa
     7501 agggattgcc ggtattaagg tgaataaagg cgctgaaagt ttttatgtcg agccttttga
     7561 acaggacgcc ggactgaata aataacaccg cattaaatct ttaaatatca catcgtttta
     7621 attatggcgc tcgcgtcagg ggactgcctg cgcctgtaaa ctgaaaataa ggaacaggaa
     7681 atatggcata tttttatttc aaattagacc gtgtgcagac aaataaatat ttcaccaaat
     7741 atcaacagac tgttttaccg ctacgcaaca gtattcttcg ggcattactg aaaaatacag
     7801 gcgctgccgg attgcgctta aagccgttcg ccatggacag aatcagtgag ttttattttt
     7861 ccggtgctct gcctgcgggc tggcgtaagc gcgatgatgt ggcttttatc ggggacggac
     7921 cgtgctttat tgccagacct gatgagttat gccctgaagg accggcgatt gccgcaatgg
     7981 ttgaagccgc tgaacgcgat ttaagaaagc atcctgattt ccttgtctgg ctctgtgaaa
     8041 agctgggggt aatgagaatc ccctccatgt ttaacacgga cccctggtgg accccttcgt
     8101 tctcccgtga tgccctgtgc gtggtgttta aagtgggcgc ttatggcagg gaaataaacg
     8161 ggtgtattcc tgaagaatgc caggagatta aacattctga atatgtggca ctgacggagg
     8221 aataattcat gattgatgca aaagtgcttg aaggggttaa aaactggctg agtatttacg
     8281 ggcgtctgac ctgcggcgtt ctggctgaaa aaatgaatat gccgccatcc tccatggttt
     8341 attttctgcg tgatgcggtt gatgccgggg tgctgacgga atgtaacggt ttttatgata
     8401 ttccgcgtcc ccgcccggtg cagccggttc gtcgcaaatg cagccaggag tctgcggctg
     8461 atgatgttca gtggtgcagc ttcagaaaat ccctgccgtg gattgaaggg catgatattc
     8521 cgtcgatggc gtgggaattt gctcagggcg ttctgacctg tgaaaccgtt tatgtggtgg
     8581 ctgaagttga tgagcaggcc atgaaagaag gcgtgcccca gtttgtgatg gcgtatatcg
     8641 acatccgcct gggcgtcatc atctgcggtt taagcggctg gaatatcact gaatatgttc
     8701 tgcgttacct gattgttgac cggacagcgg caccggccac gatttctgcg gaggtggcgt
     8761 aatgttcttt aaaacatcaa acccatccgc gctggctgcg tggcaaaaat accagcagga
     8821 ctgccagaaa gttaaggatg aggcaaaacg cctcgaggcc gtgctgaatg tttcgtgccg
     8881 gtcggtattt gaattcagta tcagtggttt ttgttttaaa gggctgcgct ttatggatga
     8941 caaatatcct tttcatcgcg acttatggcg aaaaccgact gcgtcgaatg gctggagctg
     9001 cacaccgcgc acatcacgta ttcccaaagc cctgcgcgtt gcctctgacg aacttaacag
     9061 tctgtggcgt gaatattcgc ccgtcacgta tgccagaacc gatgcactgt tgttctggct
     9121 gggcattgat ttctcggcaa tcctgtttgg ccctgtggag tggttctgcg ttgacgatgt
     9181 gatttacctt gagtgcggcg taaaacccgc aaaacagaaa atgaccgaaa ttctgtctga
     9241 tgagttttat gctgctgaaa agcgagtcag ggggtgatgc atgatgatat tacaacccat
     9301 ggggagaaaa ggccgtgcac ccgctcatgt tcgcgcatgg acacctgaag aagatgcgct
     9361 gctgattgcg ctttatccat ccaccccggt taaggatatt gctgtcagga taaaaagaag
     9421 tttctggggt gtacataacc ggattgtttt attacgcggt acttacccgg agttgctcaa
     9481 atgcaaacgc ctcagattta aacctgatga agataaattt atccgaaaaa atgccagaac
     9541 aatgaccggt aagcaaatag gagaatatct ggggcgagac cgggattctg tccacaatcg
     9601 ggcgcgatat attggcgtaa gtatgaaaaa atacggagag ttgctgccct tcacccgcat
     9661 acctgacgat gatgttcgtc ttattcgtga attacgggat gctgaatcac cacggcgtct
     9721 tacctttagg gaaatcggcg agaaatttga attatccgag ggcacggtga gttttattta
     9781 tcaccgtcgt cggactgccg aagatgttgt attacgggaa ttaatgccat gataacgaca
     9841 ttatttgttg aatcagatga accccttgtg tgtgccgtcg gaatgccggt ctgtggcgga
     9901 acgctgaccg gtgtttattt cggggatttg cgcggttatc cctggcattc actgaatgat
     9961 gctttcccgc ctgatatgga ggctgtcgtg ctgattgttc agtatggtga ccaacaggaa
    10021 ctgcgcatcg gccatatggg gtatgaaggc ttttttattg atgaagaaac cggagcctgc
    10081 cttgaagatg aggacggaca ggtgacgcac tggtgccata tttcagcttt accggaatga
    10141 cgggaggtga atgcgtgaga agttatattg ataatgaaaa actgaagaca atcagcgact
    10201 gtcttagctt gctggcaaaa ataaaagaaa ccattgaaga gattaaattt cagcttgaat
    10261 acgaaccctg tggcgatgat acatggcgta actctgccag aaaagcactg gctgtctttc
    10321 agaaacaacg ccggactgtt gaataccgcc ttgctgtatt gcgccaggag gaaaaggaaa
    10381 gaaatataag acgtcatgaa ctcgttaatg attttcttgt tcgtgaatta aaggagcgtg
    10441 tgccggagtc ggtatttttt gagtgtgagg ctgttgcccg tagcaaagca ctggaaactg
    10501 attaaacagg caggtgaatg atgagtattg aattaagatc gtcttatgaa tatcgtaaaa
    10561 ttctcatcgc cggaggcatg aaaccggaag atgcagaaaa aatcgtttct tttatggata
    10621 aagaatgtga caaacgggat atgccagaaa ttattatgga tgacatgatt ctgcattcag
    10681 ccgtagcgtt aagcccatta tggattgtgc attctcttgc tgaaattgcc aaaggcaccg
    10741 ataaacaggc agctgttgct gcccttcaga cgcttaatga aatgcgtata tctccacgcc
    10801 cgacattaat acatatgatt ctgtccagca tggaggataa agcaaatgaa tagtctaccc
    10861 gctggatggg cgcggccact aatggccagg aagcatcatt ttttttaaac aggcgaaaat
    10921 atcagtatat gtggacggtg gttatatctg gctcataatc gcgagccgga tacatttgaa
    10981 agccctgatg actgtgccga atgccgcaga agactgaata aggagaaaga taatggacag
    11041 tagttcgcag ttgttctggt atttatatat cacatccttt ttcggtgcat tcgtcattac
    11101 acgatggctg tgccggaaag ttatcagtat ttttcataaa aaatacctga ttgggcaggc
    11161 tgcgacttct ctgattaatg cactggccag atatcataag ggggaatgct actcaagaat
    11221 aaaaactgat gatggtttta atatttttgt gattcctccg gagtatcggg ttcagctcga
    11281 tcgggaaaat aatacctatc atatccgcag gagtgttgat acagcatgag agggaaactg
    11341 atatctgcca ttcatgtggc aaaacgcgag cttgccctgg atgatgaaac ctacacatcc
    11401 gtactactgg cggtcaccgg caaaaccagc tgccgggata tgtcaccgga tgagttatcc
    11461 cgcgtgctgg atgttttcaa aaaacgcggt tttaaagtgc gtcagaaccc ggttaaccgg
    11521 gccttaaaac cgggtacggt gaccgccaaa attcgcgcca tctggaaggt gatgcaccgg
    11581 cagggcttta tcaccgatgg tgcggaaacc gcccttaacc gctgggtgaa atcgcagacg
    11641 gccgcgcaga acggcggcga aggtgtggca aactggcagt ggctggagca acaccccgcc
    11701 ctggcctcag atgtgctgga gcgtctcaag cgatggcacc gccgcaaaat gctggccgcg
    11761 atgggaatgc ccgaacgcac gctgatgggg tatgacgccg tttgcaggca gtatgaaaaa
    11821 tcacttcccc gttaactcca aatcccgcca caacgcggga tttttatttt aaacttaccg
    11881 ggaacgcgag aaccggaggc tgatatggca gaaactcaga tgagcatgtt tggtggtgac
    11941 agtgagcaac tgcacgccct tatcgaccgc cttgatgaca tccctgatga tgttctcaaa
    12001 aagaactggc cgcggaccct gtctgaactg gttgaagtca ccggcgcaga acttcagcgt
    12061 caggggattg agccggtact ggccggtaaa ctggcacgca aggtggctgc ggctcaggca
    12121 gcctatatgg gcggacgggg ttattacctg ccggtcgggg aatctctctt tgccgagctg
    12181 cgaaacaatg agatattttc gcgctgggac cggggcgaga aaattgaatc ccttcgccgc
    12241 cattaccgga tgtcagaaac ccagatttat accgtcatac gcgaacagcg ccgtctgcat
    12301 ctggcaagaa cgcaaccgcc acttttctga tattccgcaa agcctgccgc ctctccttca
    12361 ccgttacgct gactcagaga acatcatgag acagcgtaac aatgccaaaa ctccccgcac
    12421 cactgcgtaa aaagctgatt gcccttgttc tggccggtgc cgggacgttc acgattgcca
    12481 cgcattacac cggttactgg gaagggaaag aaaactccac gtatatcgat cccaccggta
    12541 cacccaccat ctgttacggc cataccggcc cggatgtgaa accgggtatg accctgacag
    12601 atgaagaatg cctggaacta ctggaaaagg acatgaaatg ggcctttgcg gccattgatc
    12661 ggcgtgttca ggtgccgctt acccgtggtc agacggtggc gctggcttcg tggatcttct
    12721 gggccggtga aacgaacttt cgcaactcca cgcttctgcg cctgatcaat gccgggcaga
    12781 tgcccgcgtc ctgtaagcag tatatccgct ggatttattc aaagggggtg aaactccccg
    12841 gccttgaggc ccgccgttcg gcggatgaat ggttatgtcg ctacgacttg ccgaaagtct
    12901 gaaccgcttc tggcgaccgc tcatgatagc gctgctgtgt gcggtactgc tgttacgggg
    12961 tgtcctgtgg ctgcggtgaa taatgcctta ccggccctga ttgccggggc gtgcatggcg
    13021 gcgctgggga ttatcgctgt actggttgcc gctatatggg gaatgcacca gaagacgcag
    13081 cgccttgaag acaataatca ggtgcttgtg cgtgagcggg atgaggcgcg tcaggtgctg
    13141 gcaaatcagc agcacaccct gcaactcatt tcacaaatca gtgaggcggc cacgaatgaa
    13201 aaacaacaga acattcagca cagcgagggg cagcagagcg ttgtccgccg gtcgctggcg
    13261 gcagtgcctg cggcctctgc ccctgttcct gatgatgtgg ctgatcgggt gcgccgggcc
    13321 gtctgtgaaa tacgtgcctg tgaagccggt gccgatcccc gctgaatggc tggctgactg
    13381 cctggtccct cctgcgccgg agccgttcac gtttggggca tcggtcactt acaacctgca
    13441 actgctggcg gtgatcaaga actgcaacgt ggacaaggcc agtattcgtc gtctggaggc
    13501 gcggcgacaa catgaattta ctgatatggc cggaacgcct gctgttccgg caggaaagac
    13561 gaagtaagga aaaaggtatg gatgattcag atcgtgctca ggctgtgatg gagcggggat
    13621 cagaacgcgc cctttgtaac cggctgacac gtaagcgccg ggcaatggat gctccgggca
    13681 gacgtgtctg cgcggactgt ggcggagaga tccccgccgc acgtcttgtt gctgtgccgg
    13741 atgccatccg ttgtgtgaac tgccagaaca tcatggaggc ccgtcatgtg ggtcagcatc
    13801 gttaaagatt atgttgtgcc gatcctttcg gcgacggcaa ccgctggcgg gatattcatg
    13861 gcgctgatgc gcaaaacgtt tgtcccccgc gaggcctttg aaaaactctc cgaccgcgtt
    13921 gagaaggtgg aaacccgcct gtcgtcactg ccgacagaag ccgaggtcaa ccgtctgaat
    13981 gtggaaatcg tgaccctgcg gggtgaactg aaaaccacga acgccacgct ccgctctgtc
    14041 tcctatcaga acgaactgct gctggagcag gctgtaagga aaaaaacgca atgagtgatt
    14101 tcattactga agatcagcgt ctggtcattc tgcggtcgct ggcagattac aacggtgaac
    14161 tgggtgaatc cgtgctccag gactgtctgg atgattacgg ccaccgcgtg tcccgcgaca
    14221 ccgttcacac ccacattgcc tggcttgccg agcagggact ggtgcgtaag cgcacgctcg
    14281 ttaacggtta tttcatcgcc gaactgaccg gccggggtca ggatgtggcg gaaggtcgcg
    14341 ccagcgttcc gggtgtgaag aaaccacgcg caagggggta acgatggaca agccgacgcg
    14401 tggacgcgtg cgcaaggtgg atttgctccc cgacagcatc cgtaagccac tgctggaaat
    14461 gctgcgtgaa aaacgtctga cgcaggtcca gatacgcgag gaaatcaacc gtctgatccg
    14521 tgaggcgggc ctgccggaag agcagcagct ttcaccggcg gccatcagcc gggaagcctc
    14581 ccgtaatgag ctgattgccc gtaacctgcg tgatttacgc gagcagacaa aagccatgat
    14641 ggccgaactg ggagacaagc caaccggtga aaccacagcg ctgattctgg agatgtcccg
    14701 cgccctgatg taccgccgtc tgcgtgccgc gaccgaatcc ctgaacagtg acagcgacgt
    14761 ggatatgcgt cttattaagg acatcctcct gtcagcacag cgtgcggaaa gtgccgccga
    14821 gcgcagcatt aagcgtgaaa aagaaatccg ggccgcattt gctgaagaga tggcaaacgc
    14881 cgtcaccgac gagctgcgcg gcgtggacgg gatgagtgaa cagcttgagt cccgtattaa
    14941 ggggattctg ttgggtaaag cctgatgaat aacgccgaag aactctcccc cttactgacg
    15001 aataccgtct ccacccgcaa aattgatctg gccggtgaaa aagccctgct gggcgtggat
    15061 gtgccggaca gccttgattt gcccggtgat atgccggtat ttctggatta tcaggcccgc
    15121 tggtttgagg atgaaagcga ggtctgcatc gcggaaaaat cccgccgcac cgggcttacc
    15181 tgggcggaag ccgggcgtaa cgtcattacc gccgcaaagc ctaagcggcg tggtggccgc
    15241 aatgtgtttt atgtggggtc aaagcaggag atggcgctgg aatatatttc tgcctgcgca
    15301 ctgttctccc gtgcctttaa ccagctggca gatgctgatg tgtatgagca gaccttctgg
    15361 gatcgggata aaaaagaaga aattctgacc tacatgatcc gctttccgaa cagtggattc
    15421 aaaattcagg cactgtcttc ccgtccgtca aacctgcgcg gccttcaggg ggatgtggtg
    15481 attgatgaag ccgcgttcca cgagtcactg gatgagcttc tcaaggcggc aatggcgctc
    15541 accatgtggg gcgcgcgcgt gcgtattatc tccacgcata acggcgtcga taatctgttt
    15601 aatcagtaca ttcaggaagc ccgcgagggg cgcaaggatt acagcgtcca ccgcattacc
    15661 ctggatgatg ccattgcgga cgggctgtac cgtcgtatct gttatgtcac cggccgcgaa
    15721 tggtcaccgg aaagcgagca gaagtggcgt gatgatctct acaaaaacgc cccgacccgt
    15781 gaggatgccg acgaggaata cggctgtatc ccgaaaaaat ccggcggtgc ctatattcct
    15841 cacgcgctta ttgaaatggc gatgctccgc gacatcccga ttctgacgtt tgaagccccg
    15901 gacgacttca tcagccgtgc cgcatggctg cgtgaatcgg aggttttaac ctggtgtgaa
    15961 gaacatttaa aaccgctttt agaggcgtta aatccccgtt cccgcttcag cttcggggaa
    16021 gactttgccc gcaccggaga cctgtcctgc tttgtgctgc tggaaatcac tgaatccctg
    16081 gcaaaacgcg aggtgtttcg cgtggagctg cgcaacctgc cgtatgccca gcaggagcag
    16141 gtgatgatgt acatcctgac ccgcgttccg gcgctggtcg gtgcggcgtt cgacgccacc
    16201 ggtaacggcg gctatctggc cgaagccgcg ctgctggcct ttggcccgga catcatcgac
    16261 tgcgtgatgt tatcgccgaa gtggtatggc gagtggatgc caaaactgaa ggccgagttt
    16321 gaggatcaga atatcctcgt cgcccgccat cagaccacgc ttgatgattt gcgccatgtg
    16381 aaggtggtga acggtatccc gcaaatcgac aaggggcgta cgaaggacca gaacgcaacg
    16441 gctgtgaacg cccgccgcca tggtgatttt gccgtggcgc tctgtatggc aaaccgggcg
    16501 tcatacatgg agggctttat cctggatgaa tcagcctgcc aggcgctgcc ggagcggtcg
    16561 cgggccatag agggcggtta tcgtgatgac gatgaggcat atcatgaatt tgatcggggg
    16621 tgctggtagt gggacgcata attgatcttg acggaaaacc tttctccttt gacccggaga
    16681 tgcagagtgc cgtgctggat attccgcaga ttgccagccg ttatattgaa catccggcct
    16741 cgggtatcac cccgaaccgg gcggcacagt gccttcgcgg agctgaacgt ggcgatctga
    16801 ttgcccagtc cgatctggcc gctgacattg aagaaaagga tacccacctt tttgcagagc
    16861 tgggcaagcg acgccttgcc attcaggggg tgccatggag tattgagccg cccccgaacg
    16921 ccagcgcgaa tgagaaaaag gacgcggaaa tgctcgacga atatctgcat tccgccgact
    16981 ggtttgatgc catgctgttt gatgccaccg atgccatcct gaagggctat tcctgtatgg
    17041 agattgagca cgggatgctc ggtaaaatgc acattatccg cgccatccgc tggcgtgaca
    17101 gcgggcattt ctgccttaac ccggatgatt tgagcgaact gcggctgcgt gacggcagcc
    17161 atgccggggt ggcgtttcag ccctttggct ggatagtgca tcagtcgcgt tcacgcactg
    17221 gctacggtgg tgcgacgggg cttgtcagaa cgcttatctg gccgttcatt ttcaaaaact
    17281 attccgtgcg cgatctggct gaatttctgg aggtgtacgg cctgccgatg aaggtcggta
    17341 aatacccgtc cggggcaaca ccggagcaga aaagcgccct gatgcgggcg gtgatggata
    17401 tcgggcgacg tacaggcggg atcatcccgg ccgggatgtc gctggagttt caggcggcag
    17461 cgaacggtca ggccgatccg tttgaaacca tgatttcgtg gggggagcgt tccatctcca
    17521 aagctatcct cggcggcacg ctgaccacgg aagccggaga caagggcgca cgttcgctgg
    17581 gtgaagtgca taacgaggtg cgccgggaaa tcagggattc tgatttacgt cagctggccg
    17641 ccacgctgaa ccgcgatctg gtgtatccgc tgtatgccct gaacaccgcc cacgccattg
    17701 atatccgtcg cctgccgcgt atctgtttcc agacaaaaga gccgggggat atcaccaaaa
    17761 tcaccagtgc ggtgatgcag ctcagtacgg ggatggatat tcctgatccc tgggtacggg
    17821 agcagaccgg tattccacag cctgcccccg gtgaagccat cttccgtgtc cgtcagagtg
    17881 gcaatgaacc tgctcagaca gacaaagaga tgccaccgga aaaacaggag aagacagagc
    17941 agacagcgct ggctgccaga ctgccggaag cgaagagcag cccccgagat gaactggacg
    18001 acatggggga tgcggtgcct gcccgccggt tacaggaggc catcgacccg gtactggagc
    18061 cggtcattga agccatcaga acgcgggggc tggcggaggc actggcagac ctgcctgccc
    18121 tttaccgtga aatggatgat tcccgcctga tgacgctgct cagtgatgcc atgtttgctg
    18181 cggaaatgaa ggggatgctg gatggcacag gggattgatt taggttatgc cgccacgctt
    18241 ccctcaaaag aggcggtggc atacttccgc gccaaagggg cgcatatcag ctggaactgg
    18301 ttcgaaacag acgcggatgt tcatgcccgt tcattcacgg cggcaaaagt ggcacgcctg
    18361 gacgtactga ccacactaca ggcggaagtg cagcgtgctg ttgatgaggg gatttcacag
    18421 aaagcattta tccgcacact gacaccccgc ctgcaaaagc tgggatggtg gggaaagcag
    18481 attgtggtgg acagcgccgg taacgcagaa gaagtgcaac tgggcagtcc ccgccgtctg
    18541 gcgctgattt acaacgtgaa cacccgcgtg gcttacaatg ccgggcgtta cacgcagatg
    18601 atgaacaaca cggacacgca tccgttctgg cagtatgtgg cggtgatgga cagccgcacc
    18661 cgcccggcgc attccgccct taacgggcag gtattccgct atgacgatcc gttctggaat
    18721 acacactacc cacccaatgg ctggaactgt cgctgccgtg tccggccatt gtctcaggcc
    18781 cgtctggatg cgatggggct gtctgtttca tccggtcagg atcatctctc cacccgcaat
    18841 gttgaggctg gcgtggataa gcagaccgga gaagtcagag aaatgccggt gaccacatat
    18901 tcagatggca ccagaaccat gacaccggat gtgggctggt catataaccc cggttcggcg
    18961 gcgttcggca cagaccaggc gctgatccgt aaactgatcg aggtgaaaag cccggcgtta
    19021 cgggaaatgg tggtgcagga gatgaacaac agcccggagc ggcagctggc gttccgcatc
    19081 tgggcaaaaa acatcatgaa aacccggcga ggcggtaacg atatccgcac gctgggcttt
    19141 atgaccgaaa gtattgcgca ggcggtggaa agccggacgg gaacgccacc ggcccgcctg
    19201 ctggcgatga gcggtaaaaa tgtgctccat gcggacagcg tgaaacatca gaatgacggt
    19261 atcgccctga cgccggagga tttcgcgcag cttcccgcca tgctggctgc ccccgatgcc
    19321 gtgctgtggg atcatgtcca tcagaacctg ctttacatca ctgaaacccg tgacggaacg
    19381 gcaaagattg ccgtcaatgc gccttatggt gtgaagcgcc agccggataa gctggatgtg
    19441 gtaattaatg cttaccgggt aaataaattt gatattgaaa aagcaataga gggcggaaag
    19501 ctggaactac tggaaggtaa attgtaagcc ggtgacgggg gtcgaacccg cataaacata
    19561 tgacccctga gggtttatgc cgctttacca gttaagagta caccggcctg tatttatttt
    19621 aatcagatag ttttaaggag gcaacatgtc atccatcgat gcagccgtgg tggtggacgt
    19681 tgcgcgtctc cagcgggtct ttgcccggct tcagtttgtg ggtggtggaa aagacctggc
    19741 ccgcagtgtg gcggcgagtc ttctgtcatc gtcagaaatg gcgtttgaac aggaaaaaga
    19801 gccggacggc gaacgctggc atgactggtc agatccttac cgcaagtggc gtacccgtaa
    19861 gggatacatg cccggcaaaa tcctgacgct gaacggcgat ctggcccgac gcctgaccac
    19921 ggattatggc gacacctggg cgctgattgg atcaaatgag ccttattcgg ccattcatca
    19981 gtggggtggc ctgccaggta tgccgccggg accggcggcc attggtgcac gtccgtatat
    20041 gggctttgat caggtggcag agcaggagat catggacgaa atcagaaaac gctttaaaaa
    20101 ggccacagaa acgccttaat tgtttaaggt atgcaaatgc attaccttac ccccttcagg
    20161 cgcgtgtcgt gattttctaa cctgtattta acgggcttta aaatctgcgt gaagcgcctt
    20221 tgtatttttc ccctggaagc acttcctgaa atcccgcaaa acccgccctg aaaattcccg
    20281 gcttatgctg ccggaatgaa gacgaaaaac acgcccaaac ttgcttatgc cattctgaat
    20341 gccatcagcc tgtccgcaga cggggacggt gactggtgtc agatcatgcc agccggtcgg
    20401 gtaaaggccc gtgacggtcg cccggaaaaa ccggccgagg gctggctgat caaccatgca
    20461 gccgttgagc gcatggtctc ccgggttgtg gcgctcaatc agccggtgaa aattgactac
    20521 aaccaccaga ccctgattaa ggggcatcag gcaccggctg ccggttttgt gatggcctca
    20581 ccggagaatt ttcgcttcag tgaagagcgt ggcttcgagg tgcgcccgaa gtggaaccct
    20641 ccggcccttg aacatctgcg taataacgaa ttcccctggt tttcaccggt gattggctat
    20701 gacgagaaca ccggcgaacc tgtcgaactt cggatgctgg ctattaccgg tgaccccggt
    20761 ctgaccggca tgaatcctgt cgccgcgctg tcggcggatg acctttataa cgccttaaac
    20821 cctcctttaa aggatacctc catgaatgag caattacgcc agttgctgac ggcgctcggt
    20881 ctgaccgtgg ctgacggtga cgaatttacg ccggagctgg gcacggcggc gctgtctgcc
    20941 ctcaccggga tcaaaacccg tgcggatgca cacgacaacc tgaaaacaca ggtcgccagt
    21001 ctgtcggcag agctggaaac cgcaaaaggc acaccggctg gcggcagtat tgatctgacg
    21061 aaatacgtgc ccgttgaaac gtataacgcc ctgcgtaccg aatacgttgc gctgtcggcg
    21121 cagcacggca gcaccacgct ggagcaggtg ctggacaagg ctgaatccga agggcgcatc
    21181 ttcaaaagcg aacgcggcta tctggaacaa ctgggcgggc agattggcgt tgcggcactt
    21241 tctgcacagc ttgacgcccg ccagccggtt gcggccctga cctctcttca gaccgacacc
    21301 gtgaccgtgc cggataaaaa gactgccacc gctgcgctgt cggctgaaga tatcgcggcc
    21361 gccaaactgc tgggtaaaac cgaagccgag ttcctgaaaa tgaaagagga aatgcaataa
    21421 tgcctacccc gattacaccg gcgatgatca ccgccctgat gacgggctac cgttctgatt
    21481 ttcaggccgg gatgtccatg gccccgtcgc agtataaaaa aattgcgatg accgtgccct
    21541 ccacatcgaa atccaacacc tacggctggc tggggcagtt cccgcagttc cgtgagtgga
    21601 tcggctcccg cgtcatcgag aagatgaaag cctatggcta tgccatcgtg aacaaaactt
    21661 ttgaaggcac tgtcgccatt agccgcgatg acttcgaaga cgacaacctc ggtatttatt
    21721 ccccgttgtt ccaggagatg gggcgcgctg cggcggtaca accggatgag ctggtctttg
    21781 ctgccctgcg tgacgggatc agtgcggcct gctatgacgg tcagaacttc tttgataccg
    21841 aacatccggt ttaccccaaa gtggatggct ccggggatgc gcagatggtc agcaatatgt
    21901 ttgtggcaaa aaccggctct gtcggcgcac aggctgatta cagcggtccg gcctggtatc
    21961 tgcttgactg ctcccgcgcg gtaaaaccgc tgatttatca ggatcgccgt aaggctgaac
    22021 tggttgccca gaccaaagtc gatgaaggtc gcgcattcac cgataacgaa tttgtgttcg
    22081 gtgcatccgc ccgtcgcaat gtgggctacg gcttctggca gatggcctac atgatgcagt
    22141 caccgctgac gctggatgca ctgtggcacg gctggtcagc catgcgcgaa tttaccgctg
    22201 acggtggccg caaactcggc atcaaaccca cccatattgt tgtccccacc tcgctggaaa
    22261 aacaggcggt gcagctgctg gagcgtgaac tgttcgcaga cggaaacgcc accgtttcta
    22321 acgagatgaa gggcaagctg gagctggttg tcgcggatta tctgtaaatg gtgagccggg
    22381 cttacggccc ggcccctgcg gagggcaaat atgaatgaac atcacactgt ggcagcaaca
    22441 gatgattcag gccttcaggt cagtggtgac aattctgtca cagtgctggc agaagtgcgt
    22501 tgcagtcgtt cggcgtttcg tcgtgcgggg ttcctgttca cgcgtggacg tcagcaggtt
    22561 gaggtctccc cggagcagct tgcccgactg gaggcggagc catgcctcac tgtgcgaata
    22621 ctacagacgc ctgctgatga tgcggggagc gtggcgggtg tggttcatgc agtggccggt
    22681 acagatttag ccgaagccgg acaggatact ccccggaaaa agaccggtaa caaagcgaaa
    22741 caggcgaaaa aggcccgcgc atgaattacg ccactgaaac cgatatgcgg gcgcgttacc
    22801 gcgaggattt gctcaggccg ttactggctg tgccccgttc ggatgagccg gacacgcgca
    22861 agctgaaccg ggcgctgaca gatgcgtcag cccttatcga cagctatctg tccgcccgtt
    22921 acacgctgcc gctggaggtt attccggccg ttcttgttca gcactgttgc gcgattgcct
    22981 tttattacct gtgcgatcag cgagcctccg atcaggcgcg tgaccgttac cgtgaagcgc
    23041 tggcctggct gaaagatgtc atgaacggca atgtgccggt tggtgtggat acgaacggtg
    23101 cggcccctga atccggggat ttaccacagg ttcagtctga tgcagcggta ttcgggcgca
    23161 accagaaggg cttcatatga ttacggaaac cgaacaggca tacatcgccc gtatccgtga
    23221 gtatttcggg aatgaacttg tgtctgttga cacgcatccc ggcgactgga gcgacagcgt
    23281 actgcgcacc atgctgatta acgccccggc gatctacgtt gcctggctgg gtgccggtga
    23341 aggccgtacc cgtggtcgcc tggtcagtca ctgggtgttc tacgtcatcg gcgacatgct
    23401 caacgggcgt gaggccagcc gtcccggact gtatcagatt gtggcccggc tgatcgccgt
    23461 acttaacggc ttcagaacag aaaaaacctc accgctttac tttgaaaagg cggtcaacgg
    23521 ttacaccgaa acccaggcgg acagtggtgc ggtgatgtat gcgctttatt tctcctgcga
    23581 ggaaatgatc gcaccgctga ccgatatcag ctcgctggac gacttcctgc gtcattacga
    23641 aacctttgtg gagccgcccg gtacgccgcc gtttgaggca catattgatt tgccggggaa
    23701 cacgacaacg gcggtcgaac cgccggagga actgtaaatg aaaaccatct ttatcaaacc
    23761 cgcgccggga cgcctgattc gtgatcccga cacaatgcgc ctgctggcac aggaggggga
    23821 ggaaaagccc tttacgccgt tctggtgtcg gcgtcttgat gacggcgatg tcattcaggc
    23881 tgaaaaatcc gctgaagagg ctccggctgt atctgcggat gcaacgactg cgacagaaaa
    23941 accggtcgcc caaccggcat ccgataagga gaaaccgcaa tgattaattt tgacgggatc
    24001 ggtaatgacc gccgtatacc gctgattgag gttgagttta ataactccat ggctgtcacc
    24061 ggcactccgg cacagcgtca gtgtgtgctg ctgtttggtc aggcgagaat gaaagagaat
    24121 gccgtggacg gtgccggtgt tctggatgtt ccggtcagga tcacccgtgc gtctcaggca
    24181 acagaattct gggggcgcgg ctccatgatt gccctgatgg tggcggaatt tatcgccatc
    24241 aatcctgatg ctgaactgta tgccattgct caggggagcg gcaccggtca ggctcaggca
    24301 tgtctcatga atataattgg caccgcctct caggatggtg ttctgcacat ttatatcggc
    24361 ggtcggcgtt atctgctggc tgtcacgaaa gggcagaagg gtaaggagct tattgacgct
    24421 ctggtctctg ttattaatgc cgatgctgat gcgcccttca gtgcttatgc aggtcaggta
    24481 gcagataacg ccagcggact caagggaagt atgggaatta atgcccgttt taccggtgaa
    24541 tgttccgtac atgatatccg tgttaattat catgacggag aaaccacacc gggtggactg
    24601 accattgaac ttgggtatcc caaaacaaaa gccactaacc cggatatcac ccgaagcgtg
    24661 gcgggtatgg gcgatcgcca gtacaactat gtcgtcatgc cctataagga tgatgcaaac
    24721 ctgaaaatca tcagtgatga gcttctgaaa cgctggggac cggccaaaat gtctgacggt
    24781 gtgctgtgga tggcgcatac cggcacgttt ggtgaggtgc aggcattcgg ggcaaaacgc
    24841 aatgattttc tggcgacctg cacattcatt cccaaagccc ctgaaccgga ttacctctgg
    24901 gcagcggcgg tctgcgccac ctgtgcgccg tctctggggg cagatcctgc gcgtccgctt
    24961 cagacgctgg cgcttccgtc acgcatggcc ccggccccgg ccgatcgtct gacgcgtgaa
    25021 gagcgtaaca gtctgctgtg gggagggatt gccacggtca gtgtggctgc cggtgatgtg
    25081 gttcagatcg aacgtcaggt gaccatgtat cgccagaacg tgtacggcga aagtgatccc
    25141 agctatctgg atgtggaaac catctacacc ctctcctggc tgcgttattc cctgcgtact
    25201 tttatcacac aacgttttcc gcgccataaa ctggctgatg acgggacgcc cgttcgtgcc
    25261 ggtcagaata tcgtgacgcc ggagatcatg aagcttcagt taatttcgct gggtgaagag
    25321 tgggtggaac tcgggctggt cgaaaatctg gacaccttta aaaagaatct gcttgtggag
    25381 cgtaatgcgt cagatcgaaa ccgtcttgac gtgttatgca cgccggatct ggttaaccag
    25441 ttccgcttcc tggcagcaca gatccgtttc attttgtgag gtaaggcatg agcggaaaac
    25501 agtatcaggg cacggccact atccgtgtga acggtcagga atacgagacg cttgaggggg
    25561 ctacattttc tccgtcaggt tttgaacgtg aagtggtgaa aggcgcgaaa gtctatggtt
    25621 accgccagaa accacgtgaa gcgacgctgg actgcaaatt tccggcaggc ggagaaggtt
    25681 caccagccgc cgatgaaatc aacaactgga ctgctgtcac tatcgagttt gtggcggata
    25741 ccggcgaagt ccacatgatg acgaaggcct ggagcagtga accggcctcg cttgacggtg
    25801 gcggggagat ctccgtgaag ttcgccagcg ccaccagtac ccgtgttcag tgatcaggaa
    25861 aaataaaatg accacacgta agaaaaaaac ggcagtttcc gaggcagccg tgatggaggc
    25921 aatccgggaa gcacttgagg gggctgatcc acgcactgcc gggctgacag agcaactggc
    25981 aaagggatat gtggatctgc ttgatggtct gccgtttggt gagacccgtg aatatcgtgt
    26041 cacgttccgg gaactgacag cgaaagacag tattgacgca gaagcagaag ccgagcgcgt
    26101 ggtggagaca aacaatggcc cgatgctgat agcgtctccg tcgttacgcg gtgttgcact
    26161 gctgcgccgt cagattgccg cagtgggaga cattgaaggt ccgttgtcac cccgtcagat
    26221 tggacagtta agcgagcgcg atctctcccg cctgatggca gcggtcagcc tgctggatac
    26281 cgcgcttgcc ggaaagctgg cagctgaccg ggggcgatca ggcgcagtgt cgggatcaga
    26341 ttgaagaagc ggcaattatc ctggggatgg tgacaaaaag tggcccggaa tgggcgctta
    26401 acctccccct gtcgcaactt taccggcact gccgacagac agaaaaaatc atcagaacga
    26461 agcagtaagt atggcccgaa atctcagagc atctctgata gttgatttgc tcggcaatat
    26521 ctcagccaaa tcccgccagt ggtctcagga gctgggggca ttctcacgct ccggccaggc
    26581 ggggctgggt ggtctgggaa atgcggcccg ccgtgccggg caggaaacag atgtactcgg
    26641 aagcaggatg cagcgcacgc tggccggggt gcggggcagt atccgccatg tcacttctga
    26701 ttttgaccgt cttcagggca gtattaccgg caccatcggg cggatcagca atctttacgg
    26761 aatgctggcc ggtggtgctg ctgtatatgg ctttaaccgg ggatttattc gcccggcagc
    26821 ggagatggaa acctatatga ttcgcctgaa ctccctttac aaaggggatc gggcgaaaac
    26881 tgatgatgtc cgcaggtggg caatacagaa tgcgaaggaa accacctggg ggctggctgg
    26941 cgtcatgcag gaatatacgt ccagtctggg ctttggtatg agtgacaggg aggcccgaaa
    27001 ttttgtcacc atgcttcagg atcagggtgc cgtgggcggc tggtcactgt ccgatgcaca
    27061 gggggcatcc ctgcaactga aacagatgta tgcaagaggc agtattcagg cggcggatgc
    27121 aaatattctt gccacctacg gcattaatgc ctaccgcgtg ctggctgatc gtctgggtgt
    27181 ggaccagaaa gtggtcagaa aacttggtga aaaagggctg cttgggccgg acagtattcg
    27241 cctgttattt cagacgctgg cggaacaggc caggggagca caaaaggacg caatgaattc
    27301 ctggagtggc ctgacggcca tgatgggtga cgtctgggat cagtttgccc gggatgtgat
    27361 ggacagtggg ccatttgaaa aactgaaggg taatctcagg ggatttcttt catgggttga
    27421 cagtgccaaa tctgatggca gttaccattc ccaggcggaa agcacggcat cagccatgaa
    27481 ccagggcttt gagtatgccc gtgatgcagt gacagggttt tatcaggcga tcagaaaggt
    27541 acgcgacacg cttcaggctc tgcgggatgc gggctatggt gatgcgctgg atcgcattgg
    27601 tcagggggca cagaccgccg cgaaatacct gctgtacatg tatctcgcca cccgtgccct
    27661 gaaaatggcg cgggctgtcg gaatggggat tatacgtccg ggtgctgcat tgctgggata
    27721 cggattatca gcggcggcct tcctgacttc acctttccgg cgctctcctc cggccggaac
    27781 accaccaggc acatctcagg gacgagggca acgttttata aactttctta ccggggtaaa
    27841 tccggcagcc gttcagccag tacttgtaac aaactggccc acaggaggac tgtccggcac
    27901 cggcgcgacc gatacttcct caggcggacg tcaggggcgg ggccgcagga aacgcggacc
    27961 ggggcgcgga agaccggtca cgcctcccct gccaccgtcc ccagtgccac cgtctccctc
    28021 ttctggtggg gcagggttct gggggcgcat gatggggcgc gccggtgggt tactttcgtc
    28081 tgtcggtagc cgcatggggc tggggcgttt ctccggcttt ttccgcagtg ccggtggact
    28141 ggccggccgg ctgggtggcg gtgccctgtg ggctggcgcg atggcagccc cggttctgct
    28201 ggatggcagc gccagtgcca cagacaaggg agaagcggtc ggctctcttg caggcagtat
    28261 tgccggtggt gcgctggggg cggcagccgg acctgtgggg attgccattg gttctacggt
    28321 gggcagctat ctcggtaact atctgggggg atggctgact gaagcctggc agaaattacg
    28381 gggcagtgat gatgaaagca gtggacaggc ggtacaaaaa gcctctgccc gtgtggagct
    28441 ggttgccccg gaagggtggc aggctcgcag tattgatatt gatgacacgt ccggtcacgg
    28501 actggatgtg aatgtctgga acggagggaa ttatggcctc tggtgacggt cgtggtgcgt
    28561 tccgtggtgt gccgttcctc gtctggcgtg agcagcgcga acgtggcggg cgaaacattg
    28621 tccgtcggga atatccgtta cgggaaaccg gtggggcaga cgatctggga cctaaactgg
    28681 ctgaatttac gttcagtgta cttgtgatgg gggatgatgt tcagacgcag cgaaatcgtc
    28741 tgcgtgatgc ccttcgtgct cccggtgccg gggagctgct tcacccggat tacggcacgt
    28801 taaatgtgct gataaacagc tttgaaagtc gttataacgc cgctgaacag ggcgtggttg
    28861 agttcaccat taatgtcacg ccggtaagtg atgataccgc acctgctgtc acacaggaca
    28921 cggccgccat tctggagcag aagagtacca ccgcgctcgg gcaggttttt gaaaccctgg
    28981 aagcgggctg gacggtgatc tctgacggaa tgcacgatgt ccaggcaatg accgaaacca
    29041 tcagtgataa ggtgtccgcc ctggaaaatg ccgtttccgg catggggatt gtacaggata
    29101 tcagtgcctt tactgccacg ttcacggcgc tgaaagggaa tgccacggca ttactgacag
    29161 caccttcccg catggcctca tcttttgcgg ggcttttcag tgccctgatc accctgccat
    29221 cgctgccgtc actgtcttca ggaggcctga atacccggcc cggtggcagt atcggccgga
    29281 cgacatccgc cgtctctcag ggaatgccac aactgtacag gacattatct tccctgcgtt
    29341 acgtgctgga tgagcaggat aatccgcaga ctctcatcgg tctgacaccg gcagcacaaa
    29401 agaatattcg tctgatacgg gcggtaatgc agagtgctgc cgtggtggcc caggcacaga
    29461 ccgtgggaaa actgctggat caggttctca gtcaggaaac cctgccggac agtgacgcag
    29521 cccaccgtac ctggcctgtc tggctggaaa gttcagttga tcttcagcgc attaaccgtg
    29581 acttaagcga agcgctcgaa cggcaggtga tgacactgtc cgggcagggg tataccgcca
    29641 cggcgctgac gcttcgtgat gccagactgg cactgacaga ggatctgaat acacggggag
    29701 ttcagctacc cggtgcaaca gtagtgactg tacgtaccac cgagcctgca ctggtgaccc
    29761 tgtaccgtgc caccgggaac agtaccggct ggcaacgttt tgtgcgccgt aacggtattg
    29821 ttgatccgct gttcattccc ggaggccatt cagtggaggt gattagtgag cagcagggtt
    29881 gaactgtatc tgggcggtga gattttttcc ggctggctga cggtgagtgt tcgtcgctct
    29941 cttgaacatc tggcgggctc ctttgaactg ggggtaatga tgcccggtgt acgccttccg
    30001 tcatccgtcc gtgccggtca gtctctggaa ttgcgcattg acggtcagcc tgtgatcact
    30061 ggctggctgg atcaggtccg gcagcgcatt agcgccacgc gttttcagat cacgctcagc
    30121 ggacgggata aaaccggtga cctggtggac tgttcagcca ttcatccggg cagccagtgg
    30181 aggaaccgca cgctggagca cattgcttca gatttgtgtg ctccgttcgg ggtcacggtg
    30241 cgctggcagg taaatgatgc aacggcagcc cggccctttt ccaccttcac actggaaaat
    30301 tcagaaaccg tggcagatgc gctgacacgg gccgcgagac acagaggcgt cctggtaacc
    30361 agtaatgccg ccggtgaact ggttttcact caggccggca gtcagcgtgg cgacacgctg
    30421 acgctgggcg aaaatctgct ggatttggat cacaacgtgg atcaccgtct gcgccacagt
    30481 gaataccgtg tacgggggca tgggcgtggt ggtggtcatg ccggggatgc actgacagcc
    30541 ggaacgctgg ccgcaccagt tggtacggtg acagacagtg ccatccaccg ttacagaccg
    30601 aaaattgtgc tggcggatca tgctgttgat gcagacggtg cacgccagag ggctgtccgg
    30661 gaaatgcgcc gggcggttgc ccgctctgtg cgcctgacag ccaccgtgcg gcactggttt
    30721 cgggagaacg gccaattgtg ggatatcaac ctgctgacgg ctgtcacggc tccccgcacc
    30781 ggagtggaag agcgtgatct tcttgtctgt caggtggagt tttcgctgga tgcaaatcac
    30841 ggcgaaacca cccgtctgat tctggcaccc cgtgacggct ttattgttcc ggcagagccg
    30901 ggaaacagcg gaagcggaaa tgcgggtgac gtggacgcct tcgtgcgggc acagatgaaa
    30961 aaacagggga ttaaattcaa tgatgaatga cgaagtcatc agccgccttc tggcccccgt
    31021 gatgcggggt gttcgtctgc tgttcgggcg tggtgtactg accggcacaa cggacacgct
    31081 gaaaatccag aatgtgcaaa tcaccggtat ggatggtgaa acctttgatg acgttgaacg
    31141 cccccagcag tacgggcaaa tcagcgtccc cctgcctggt gcggaagttt ttctggcctg
    31201 tgctggcgga caacgggatc aggccgtggt gcttgtggtg gaagaccgcc gcagtcgccc
    31261 gaccggactt accgccggag ataccggcgt gtatcaccat gaggggcacc gtatacgtct
    31321 gacaaagaac ggccggatca ttgtgacctg taagacgctg gagatttacg ccgatgaagg
    31381 tgttcaggtg gatacaccgg aggctcactt taccggtaat atcacagtgg ataagaacct
    31441 gcatgtcaaa ggcaatgtgt ccattgacgg caccggaaga tcacaggggg cgttcacgat
    31501 gtccgaagcg gttattgccg ggatcaccta ttcaggtcat gtgcatcacg ataacggtga
    31561 aggcagcaaa acgggaggac cagagaatgg ctgatattgc tgtcgtctgg gatcagggtt
    31621 gtggttccct gcaactgaac ggcgcagatc ttctgacgga taacagcctg ctgactgcgg
    31681 tcattatttc actgtttacg gacaggcggg cgctggattc cgatgaaatc cctgacggca
    31741 cccgtgaccg tcggggatgg tggggagaca gtttccggga gcgccccatt ggctcccgtc
    31801 tctggctgtt aagccgtgaa aagacgctgt cctccgtggt cagccgtgca caggcctatg
    31861 ctgatgaagc gctggcgtgg ctgcataaaa gcggtgttgc cacatccgtg gtatgtcatg
    31921 ccatgcgtgt ggggcatgat cgcctttcgc tttccgtgaa aatcaccctg ccggacggaa
    31981 gcagacatcc gatgattttt tatgctgata tgaaggggga atgatgcctt atcagccctt
    32041 accactggcg caactgatca cacagacaca acaggatatc agccagcgcc tgcccggttc
    32101 gcagccgggc gtgaatgaaa ccaccctgaa tgccattgcg tatgcactgg cgggtctgtc
    32161 agcacaggag catgaacatc tggcctggat ctcccggcag ataattccga cagaagctga
    32221 tgaagccgaa ctcctgaaac actgcgcatt ctggggtgtc atccgtaaac cggcttcccg
    32281 cgctgacgga ccggtacaac tgatgctgac cacggatgca gggatcacgg aaggcgtact
    32341 ccttcagcga agcgatggtg ttgtgtaccg catcaccggc tctgcgaccg gaaaagccgg
    32401 aacactgaat gttaatgtgg aggcggaaag tgcggggcgc gccggaaata ccccgaccgg
    32461 aacccgcctg tcctttatca cgccacaggc gggcatcaac cagacagcca cggttaccgg
    32521 cacgggactc accggtggtg cggatgtgga aacggtgccg gagttgctgt ccaggctggt
    32581 attccgggta cagaacccgc catcaggggg aacacagtat gattttgaac gctgggcacg
    32641 ggaagtaccg ggcgtgacgc gggcatggtg taagcctgaa tggcctgagg cgggtagtgt
    32701 tggtgtgact tttgttcagg ataataaccc tgacattttc cccggagaag gtgatgtgaa
    32761 gcgggtggcg gattatatcc gcagtcatga tgatccggcg acgggccagc ccgttggtca
    32821 gccacttggg ccgacaatca gcgtgtttaa gctgaccaat aagccggtgg cgtttgagat
    32881 caggattgta cccaaaacgc cggaaaatca ggctgccgta aaacaggcat taacggacct
    32941 gctttataac gaatcgcggc cgggtggact tgtattgcct tcatcattct ggcgggctgt
    33001 tgcaggggtg aaaggactgg aggattttga agttcgcagt ccgctgaagt ccgtgatggc
    33061 cggagataca gagttgctga ctgtggggga aatcacatgg ctgtaaccct gaccccgcat
    33121 cagcgcgccc tgttgcagtt gctgcctgac gggctggcat gggataagcg gccgtcatcc
    33181 gttcttgcag ctttgtgcct gggcctcagt cattccacgg agcgtgtttc ctggaccggt
    33241 aaccagatgc tggcagaacg ttttcctgat tcatcccgtc tgctgctgga agactgggag
    33301 cgttatctgg ggttaccgga atgtgatatg accggcgcaa ccattcagga acgtcagcgt
    33361 tatgcaggga ataaataccg gatgaaaccc tctcttaacc gtgaatttta tatccggttt
    33421 gcggcagagt ttggttatga aatagatatt cagccatcac cggattcaca gtgggtcagt
    33481 attgtcacga taaacagtga aaccggctac cggaatatga atgtgctgga tgatattctc
    33541 accccgctgc gtatttatga gggtggtgcg ctggaatgta ttctgaaccg ttataagcct
    33601 gcatggcaga cgtttattta cgtatatgca aacagtcacg aagaggagaa tatttaatgt
    33661 tccatgttga taataattcc ggcgtggcga atatgcctga actggctccg gcgcagagta
    33721 ataccaccac ctggtttacc gaaggtgacg gacaaaaagg tatcagctgg attggtcagg
    33781 actggctgaa tattctccag gccgaactgc tgaatattct ggctgaagcc agtattcagc
    33841 cggataaggc gcagttaaac cagcttacgc tgtccattaa agccattatc gctgcgaatg
    33901 ccttttcccg gaaaaacaac ctgaaagaaa ttgctgatgc cggtgcggaa gcccagcgtc
    33961 tggcccgtgg ttatcttggt ctggggacgc ttgccacaaa aaacagtctt ggtcccgctg
    34021 acgttaatgc cctggcgaag gatcagaacc ttgccgacct ggagaatgcg gaaaccgccc
    34081 gtaataatct ggatgtttac agcaggagcg aaggtgataa ccgttacctg cgcagggacc
    34141 agaatggcgc ggatatccag gacaaaggaa cctttatcga taacctcggt ttacgggaaa
    34201 cggtaaataa ggctgctgat gccctgccat cggctggaac cgccgtggca gcaaacagac
    34261 tcgccacccc aagaaatatt aatggtgttc cttttgacgg aacgctggat atcaacatca
    34321 cgtcaggaat gacgcagtca accgcagatg gccggtatgt acagaatgtt cagcttggag
    34381 cacagagcta tcattcaccc ggaggcaatg aaatgtcatg gaattacagc gcaccttccg
    34441 gttgtatgct ttccggtatt gacgtgcagg aaaccggctc ccggtctgcg gacaatatcg
    34501 gtggggtcta ttatcgcccg gttcagattt atattaataa tgcctggaga acggtaagtt
    34561 cagtgtaaac cacagaaaag ggtgctgaat gcaccctctg aattattccg gtttttccgg
    34621 ccagggaatc gcctggtaat gcccctcact ggcaatatca ttaaagtgca tttctttaag
    34681 ctgtcggata tacgccatcc agcgcgtcag ctgtaattta ttttcatcgc taatcatgtc
    34741 cagttttaat tctgttttcc agtcatccac ggtttcgttg gcttcctgga gcagcgatga
    34801 gcgacgcttt tcagcaaccg tcttccagtt taccggcacc tttgatatca ggccatcaga
    34861 aaactgccag ttaccgtcaa tattcacacc ttccggcagt tcgtccactt caacaacagt
    34921 aaaacctgcc ggatataaag cagagacatc ttcagcaaca gagcagataa tacccgttac
    34981 aggcgaaata cagagtttgt atttctttgt aaaaagcggg atagattcat aaaaatccct
    35041 tccgtcctca ctctggaaat actgaacatc ctcaccatag ggtttgtctt tcgggtaata
    35101 tcgtttgaca ttaatcagtt gcataaacat caccatgtaa atcaatgata aaaacaggaa
    35161 taatactatc gccatataca ggcaatgctt gtctgaaata acgcatattt cagatgctgg
    35221 ctgctgtgtg ctatgccccc gttaattaaa tacttgcagc ggatacccaa tgcccattaa
    35281 tacaatattg tatctgtctg taataaacac ggtcatcacc accacgcatt tcccagtctc
    35341 cgtcagtctt aaaaccggta atcacatttc ctgatgacag ggagtaatta tattccagtc
    35401 cgccgcttaa tgcctgcgct cccagtctta cccctgtaac ataacgctga ttggccgttg
    35461 attccggcat tcctgacgta atgtttatat cctgcgtgcc gtcaaaggcg acaccattga
    35521 ttctgcaggc atttgccagc ctgcttgctg caacagccgt accgtttgat ggcagggcat
    35581 tcgcggccct gtttaccgta tcccgtaaac cgaggtttca gggatgccgt atatctgctt
    35641 taaaaggctt ttccacctct ttttaaggaa ggtaaataca tgctgattgg ctatatacgc
    35701 gtatcaacaa atgaccagaa caccggttta caaagaaatg cgctggaatg tgcaggatgt
    35761 gagcagattt ttgaagacaa aatgagtggt aaaacgacac agcggccggg actgaagcgg
    35821 gcactgaaga aactaaaacc cggtgacacg ctgatggtct ggaaactgga caggctgggg
    35881 cgcagtatgc gccaccttgt tgcgctgaca gatgaattac agcaacaggg gataaatttt
    35941 cgtagtctta ctgacaacat tgatacgtca acccccatgg ggcgcttctt ttttcacata
    36001 atgggtgcgc tggcagaaat ggagcgtgaa ttaattgtcg aacggacacg ggctggtctt
    36061 gcagctgccc gtgaaagggg acgaaccggc ggcagacccc gccgtctcac gcaggaacaa
    36121 tacgagcagg tcgggcgttt actggccggg ggtgtcagtc gaagacaggt ggcacttatt
    36181 tttgatatcg gtctttcaac cctttatcgt tattatcctg cgagcgtttc ggaaaaataa
    36241 gacactctct ttgttgggcc tttactgaat taacgcaaaa tggcgtttat cggtctgtgg
    36301 atatgataat tcagtttatt caggaacagg ctgattatta tgacgtccaa atgggttcaa
    36361 ctatcatcaa tgcccggaaa ctttactgtt aaagtttccg gtggtacagc ggcatttctt
    36421 gaggctccct ttcctccggc cgaaacaaaa ggaggaatga catttgctga ctgtctcatc
    36481 agttttaata cccgggattg cctgtgggta aggccagtat ccggtgatcc gagtgtggaa
    36541 attaccggag cgggtattgg tgcggtcatt ccgttaagtg ctgatgttgc cggtactgct
    36601 gaaccgtcag actgggataa tgctgaaaca catacccgtc cgtcaggaaa tgaaactgct
    36661 tccagctccc cttcctggta ttacgtggtg gttcttgctg gtcagtcaaa cggtatggct
    36721 tacggtgaag gtctgccact gccggagaca tatgaccgcc ccgaaccgcg tattatgcag
    36781 ttagcccgtc gcagtacagt cacgccggga ggtaaagctt gtcagtataa cgacatcatt
    36841 ctggccgacc actgtctgca tgatgtgcag gatatgagtg gaaaaaatca cccgaaagcg
    36901 gatgtggcta aagggcaata tggcaccgtg ggtcaggggc ttcacattgc caaaaaactg
    36961 ctgccgttta ttccggcaga tgcggggatt ctgctggttc cctgctgccg tggtggttct
    37021 gcattcacgg tgggtgctga cggcacgtac agtgacagca cgggtgcttc agaggactca
    37081 gcccgctggg gtgtggataa gccgctgtat aaggacttaa tcagccggac aaaagcggca
    37141 ctggcgaaga acccgaaaaa ccgcctgctt gctgtggtgt ggatgcaggg cgagtttgat
    37201 attgatgcga agccgacgga gcattctgcg ctgtttctgg cgatggtgga aaaattccgc
    37261 gcagacctgg ctgaacaggc ggaacagtgt accggtggca gtgctgctgg cgttccgtgg
    37321 atttgtggcg ataccacgta tttctggaaa cagaaaaatg aaccggcata ccaggcaatc
    37381 tatggcggct acaaaaacaa aacagataaa aacattcatt tcgttccatt aatgacagat
    37441 gaaaatggcg tgaatgtacc caccaacaat ccggcagaag acccggatat tgaatccatt
    37501 ggttattacg gctcaacgtg gcgtaacagt gccgccacct ggacatctgc ggaccgggcc
    37561 agccatttca gttcatgggc acgtcgtggg attatttccg accgtctggc cacggcagtt
    37621 ctgactcatg cggggcgaac cacagtaaaa gccgatgttc catcttctga aactgaagca
    37681 ccagtgccgt caccttcaga aactgaagca gtaaccacaa cactgctgtc ttaccgtgtc
    37741 agtgagtcag aaggaaatct gaaagcccag ggctgggagc cggctggcgg taaagctgaa
    37801 attatcagtg atgcaggagg cacaggtggt aaggcaatga aactgaccaa ggaaacaggt
    37861 aaatcatcct ggtatctgga tcatgatgcc ggtactggtt ctgaactgct gaaaaatggt
    37921 ggtttaatca gttgtcgttt taaagtaccg ggcgacctgg tggcaaacca gtatgtcatg
    37981 gcgctttact ggccggtttc ctctctgccg cagggtgtca ccctgacagg ggatgcaggg
    38041 aataatctgc tggcatcgtt ctacatccag acagacgcaa aagacctgaa tgtgatgtac
    38101 cacaatgcaa aagtggcaac aaataaccag aaactgggaa cctttggtgc atttgataat
    38161 gaatggcata cgctggcctt ccgttttgca ggaaataaca gccttcaggt tattccggtt
    38221 attgatggtc aggatggtgc cgcgtttacc ctgacgcagt caccagtagg aactttcccg
    38281 gtggacaaac tccgcgtaac ggatatcaca aaaaatgcaa cttacccggt gctgattgac
    38341 agcattgtgg tggaagtgaa aaaagcggta acagaataaa aaaatcccgc cggtcatgat
    38401 atggctggcg ggagctacct aacccataaa ggaaaataaa aatactcccg aaggagtggt
    38461 tatcagtcgc ttcgatgttt ttacccgata acgaattatg tagtcaatat caaaggtaag
    38521 gttatatgac attcgttcat acaatgctgc tttatttctg cgcagtggtc agtgcgttat
    38581 atctggtgag cggcggttat aaggtcatca gaaattatat tcgccgtaaa attgatgatg
    38641 cggcagctga aaaactcagt aaaacagcac aggcaccgtc atccccaaac gacccgaccc
    38701 cgctctgata acggggcaat atacctgcaa aaggagaata ttcatgccag agattaaagg
    38761 cacggttact gaagagcttg tcaaacaggc actttattct gaagaagtga accgcgtgct
    38821 gaaggcacag gttcgcaagg attttgaggc acaaatcgac gcatatgttg atgaagtgct
    38881 ggcccgaatg gttggccgtt ctccggctga aaacagcacg gaaaataagc ctcaacccgt
    38941 agagcaacca gagccggttc agcccggaac tgacggtgct atgatgtaac aataccgacc
    39001 ggcaggtttc cctgccgggt gaatttgtta atgcacgttt tgtgtatttt ccctccgggg
    39061 agaacaggca ggacatatca gcatcctgaa acaggaagca ttttttggtt tgtatgtctt
    39121 gccattttga gatcgagaac ggcgatagat gagctgtaag ggagtcttac aggcagggca
    39181 aacaggctct ttatccatga taaagatctc ctttttatgc atctctgctt tacaggagac
    39241 cggggaatat cataggatac gccacgagct cgaagtaaag cattgatgca aaattgtcta
    39301 aatcggccca gaatagttcg cgctatctgg gtcgcccttt atgtatgtag tatcctgttg
    39361 atttgaaaca tactacatat acgcgcgatc ct
//
LOCUS       DAZFWL020000030        39182 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFWL020000030 DAZFWL020000000
VERSION     DAZFWL020000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.030"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(149..340)
                     /gene="yfgG"
                     /locus_tag="ACHKNP_RS08480"
                     /old_locus_tag="pgaptmp_001696"
     CDS             complement(149..340)
                     /gene="yfgG"
                     /locus_tag="ACHKNP_RS08480"
                     /old_locus_tag="pgaptmp_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HGU4703884.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            358..510
                     /locus_tag="ACHKNP_RS08485"
                     /old_locus_tag="pgaptmp_001697"
     CDS             358..510
                     /locus_tag="ACHKNP_RS08485"
                     /old_locus_tag="pgaptmp_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703885.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            692..2935
                     /gene="pdeF"
                     /locus_tag="ACHKNP_RS08490"
                     /old_locus_tag="pgaptmp_001698"
     CDS             692..2935
                     /gene="pdeF"
                     /locus_tag="ACHKNP_RS08490"
                     /old_locus_tag="pgaptmp_001698"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HGU4703886.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2974..4515)
                     /gene="ppx"
                     /locus_tag="ACHKNP_RS08495"
                     /old_locus_tag="pgaptmp_001699"
     CDS             complement(2974..4515)
                     /gene="ppx"
                     /locus_tag="ACHKNP_RS08495"
                     /old_locus_tag="pgaptmp_001699"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HGU4703887.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4520..6586)
                     /gene="ppk1"
                     /locus_tag="ACHKNP_RS08500"
                     /old_locus_tag="pgaptmp_001700"
     CDS             complement(4520..6586)
                     /gene="ppk1"
                     /locus_tag="ACHKNP_RS08500"
                     /old_locus_tag="pgaptmp_001700"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HGU4703888.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANVQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6757..7395)
                     /gene="purN"
                     /locus_tag="ACHKNP_RS08505"
                     /old_locus_tag="pgaptmp_001701"
     CDS             complement(6757..7395)
                     /gene="purN"
                     /locus_tag="ACHKNP_RS08505"
                     /old_locus_tag="pgaptmp_001701"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGU4703889.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7395..8432)
                     /gene="purM"
                     /locus_tag="ACHKNP_RS08510"
                     /old_locus_tag="pgaptmp_001702"
     CDS             complement(7395..8432)
                     /gene="purM"
                     /locus_tag="ACHKNP_RS08510"
                     /old_locus_tag="pgaptmp_001702"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGU4703890.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTQLDGKPL
                     ADYLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8757..9383
                     /gene="upp"
                     /locus_tag="ACHKNP_RS08515"
                     /old_locus_tag="pgaptmp_001703"
     CDS             8757..9383
                     /gene="upp"
                     /locus_tag="ACHKNP_RS08515"
                     /old_locus_tag="pgaptmp_001703"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGU4703891.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9469..10758
                     /gene="uraA"
                     /locus_tag="ACHKNP_RS08520"
                     /old_locus_tag="pgaptmp_001704"
     CDS             9469..10758
                     /gene="uraA"
                     /locus_tag="ACHKNP_RS08520"
                     /old_locus_tag="pgaptmp_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HGU4703892.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHHSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10853..11554
                     /gene="hda"
                     /locus_tag="ACHKNP_RS08525"
                     /old_locus_tag="pgaptmp_001705"
     CDS             10853..11554
                     /gene="hda"
                     /locus_tag="ACHKNP_RS08525"
                     /old_locus_tag="pgaptmp_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HGU4703893.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11692..12051)
                     /gene="arsC"
                     /locus_tag="ACHKNP_RS08530"
                     /old_locus_tag="pgaptmp_001706"
     CDS             complement(11692..12051)
                     /gene="arsC"
                     /locus_tag="ACHKNP_RS08530"
                     /old_locus_tag="pgaptmp_001706"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HGU4703894.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12072..13535)
                     /gene="bepA"
                     /locus_tag="ACHKNP_RS08535"
                     /old_locus_tag="pgaptmp_001707"
     CDS             complement(12072..13535)
                     /gene="bepA"
                     /locus_tag="ACHKNP_RS08535"
                     /old_locus_tag="pgaptmp_001707"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HGU4703895.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13748..14809
                     /gene="yfgO"
                     /locus_tag="ACHKNP_RS08540"
                     /old_locus_tag="pgaptmp_001708"
     CDS             13748..14809
                     /gene="yfgO"
                     /locus_tag="ACHKNP_RS08540"
                     /old_locus_tag="pgaptmp_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU4703896.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14847..15695)
                     /gene="focB"
                     /locus_tag="ACHKNP_RS08545"
                     /old_locus_tag="pgaptmp_001709"
     CDS             complement(14847..15695)
                     /gene="focB"
                     /locus_tag="ACHKNP_RS08545"
                     /old_locus_tag="pgaptmp_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HGU4703897.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(15717..17729)
                     /gene="hyfR"
                     /locus_tag="ACHKNP_RS08550"
                     /old_locus_tag="pgaptmp_001710"
     CDS             complement(15717..17729)
                     /gene="hyfR"
                     /locus_tag="ACHKNP_RS08550"
                     /old_locus_tag="pgaptmp_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HGU4703898.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17759..18172)
                     /gene="hyfJ"
                     /locus_tag="ACHKNP_RS08555"
                     /old_locus_tag="pgaptmp_001711"
     CDS             complement(17759..18172)
                     /gene="hyfJ"
                     /locus_tag="ACHKNP_RS08555"
                     /old_locus_tag="pgaptmp_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HGU4703899.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(18165..18922)
                     /gene="hyfI"
                     /locus_tag="ACHKNP_RS08560"
                     /old_locus_tag="pgaptmp_001712"
                     /pseudo
     CDS             complement(18165..18922)
                     /gene="hyfI"
                     /locus_tag="ACHKNP_RS08560"
                     /old_locus_tag="pgaptmp_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18919..19464)
                     /gene="hyfH"
                     /locus_tag="ACHKNP_RS08565"
                     /old_locus_tag="pgaptmp_001713"
     CDS             complement(18919..19464)
                     /gene="hyfH"
                     /locus_tag="ACHKNP_RS08565"
                     /old_locus_tag="pgaptmp_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HGU4703900.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPLAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(19474..21141)
                     /gene="hyfG"
                     /locus_tag="ACHKNP_RS08570"
                     /old_locus_tag="pgaptmp_001714"
     CDS             complement(19474..21141)
                     /gene="hyfG"
                     /locus_tag="ACHKNP_RS08570"
                     /old_locus_tag="pgaptmp_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HGU4703901.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDHRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(21131..22711)
                     /gene="hyfF"
                     /locus_tag="ACHKNP_RS08575"
                     /old_locus_tag="pgaptmp_001715"
     CDS             complement(21131..22711)
                     /gene="hyfF"
                     /locus_tag="ACHKNP_RS08575"
                     /old_locus_tag="pgaptmp_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HGU4703902.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22716..23366)
                     /gene="hyfE"
                     /locus_tag="ACHKNP_RS08580"
                     /old_locus_tag="pgaptmp_001716"
     CDS             complement(22716..23366)
                     /gene="hyfE"
                     /locus_tag="ACHKNP_RS08580"
                     /old_locus_tag="pgaptmp_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HGU4703903.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23378..24817)
                     /gene="hyfD"
                     /locus_tag="ACHKNP_RS08585"
                     /old_locus_tag="pgaptmp_001717"
     CDS             complement(23378..24817)
                     /gene="hyfD"
                     /locus_tag="ACHKNP_RS08585"
                     /old_locus_tag="pgaptmp_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HGU4703904.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASKVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24834..25781)
                     /gene="hyfC"
                     /locus_tag="ACHKNP_RS08590"
                     /old_locus_tag="pgaptmp_001718"
     CDS             complement(24834..25781)
                     /gene="hyfC"
                     /locus_tag="ACHKNP_RS08590"
                     /old_locus_tag="pgaptmp_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HGU4703905.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25792..27810)
                     /gene="hyfB"
                     /locus_tag="ACHKNP_RS08595"
                     /old_locus_tag="pgaptmp_001719"
     CDS             complement(25792..27810)
                     /gene="hyfB"
                     /locus_tag="ACHKNP_RS08595"
                     /old_locus_tag="pgaptmp_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HGU4703906.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27810..28427)
                     /gene="hyfA"
                     /locus_tag="ACHKNP_RS08600"
                     /old_locus_tag="pgaptmp_001720"
     CDS             complement(27810..28427)
                     /gene="hyfA"
                     /locus_tag="ACHKNP_RS08600"
                     /old_locus_tag="pgaptmp_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HGU4703907.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28680..29150)
                     /gene="bcp"
                     /locus_tag="ACHKNP_RS08605"
                     /old_locus_tag="pgaptmp_001721"
     CDS             complement(28680..29150)
                     /gene="bcp"
                     /locus_tag="ACHKNP_RS08605"
                     /old_locus_tag="pgaptmp_001721"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HGU4703908.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29150..29722)
                     /gene="gcvR"
                     /locus_tag="ACHKNP_RS08610"
                     /old_locus_tag="pgaptmp_001722"
     CDS             complement(29150..29722)
                     /gene="gcvR"
                     /locus_tag="ACHKNP_RS08610"
                     /old_locus_tag="pgaptmp_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HGU4703909.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29868..30746
                     /gene="dapA"
                     /locus_tag="ACHKNP_RS08615"
                     /old_locus_tag="pgaptmp_001723"
     CDS             29868..30746
                     /gene="dapA"
                     /locus_tag="ACHKNP_RS08615"
                     /old_locus_tag="pgaptmp_001723"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGU4703910.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30763..31797
                     /gene="bamC"
                     /locus_tag="ACHKNP_RS08620"
                     /old_locus_tag="pgaptmp_001724"
     CDS             30763..31797
                     /gene="bamC"
                     /locus_tag="ACHKNP_RS08620"
                     /old_locus_tag="pgaptmp_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HGU4703911.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            32010..32723
                     /gene="purC"
                     /locus_tag="ACHKNP_RS08625"
                     /old_locus_tag="pgaptmp_001725"
     CDS             32010..32723
                     /gene="purC"
                     /locus_tag="ACHKNP_RS08625"
                     /old_locus_tag="pgaptmp_001725"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGU4703912.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32877..33104
                     /gene="vapB"
                     /locus_tag="ACHKNP_RS08630"
                     /old_locus_tag="pgaptmp_001726"
     CDS             32877..33104
                     /gene="vapB"
                     /locus_tag="ACHKNP_RS08630"
                     /old_locus_tag="pgaptmp_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HGU4703913.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            33104..33502
                     /locus_tag="ACHKNP_RS08635"
                     /old_locus_tag="pgaptmp_001727"
     CDS             33104..33502
                     /locus_tag="ACHKNP_RS08635"
                     /old_locus_tag="pgaptmp_001727"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4703914.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            33649..34509
                     /gene="ypfJ"
                     /locus_tag="ACHKNP_RS08640"
                     /old_locus_tag="pgaptmp_001728"
     CDS             33649..34509
                     /gene="ypfJ"
                     /locus_tag="ACHKNP_RS08640"
                     /old_locus_tag="pgaptmp_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HGU4703915.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGRMQQ
                     QGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDP
                     AQCNTFGKSI"
     gene            34524..36539
                     /gene="tmcA"
                     /locus_tag="ACHKNP_RS08645"
                     /old_locus_tag="pgaptmp_001729"
     CDS             34524..36539
                     /gene="tmcA"
                     /locus_tag="ACHKNP_RS08645"
                     /old_locus_tag="pgaptmp_001729"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HGU4703916.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            36613..37311
                     /gene="ypfH"
                     /locus_tag="ACHKNP_RS08650"
                     /old_locus_tag="pgaptmp_001730"
     CDS             36613..37311
                     /gene="ypfH"
                     /locus_tag="ACHKNP_RS08650"
                     /old_locus_tag="pgaptmp_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4703917.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(37421..37621)
                     /gene="ypfN"
                     /locus_tag="ACHKNP_RS08655"
                     /old_locus_tag="pgaptmp_001731"
     CDS             complement(37421..37621)
                     /gene="ypfN"
                     /locus_tag="ACHKNP_RS08655"
                     /old_locus_tag="pgaptmp_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HGU4703918.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(37649..38776)
                     /gene="dapE"
                     /locus_tag="ACHKNP_RS08660"
                     /old_locus_tag="pgaptmp_001732"
     CDS             complement(37649..38776)
                     /gene="dapE"
                     /locus_tag="ACHKNP_RS08660"
                     /old_locus_tag="pgaptmp_001732"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HGU4703919.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAANLQLLARMYQRIMEQLVA"
     gene            complement(38780..39136)
                     /gene="yffB"
                     /locus_tag="ACHKNP_RS08665"
                     /old_locus_tag="pgaptmp_001733"
     CDS             complement(38780..39136)
                     /gene="yffB"
                     /locus_tag="ACHKNP_RS08665"
                     /old_locus_tag="pgaptmp_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HGU4703920.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9584 a        10033 c        10296 g         9269 t
ORIGIN      
        1 atttcgcagc caaacaggat aaacatcagt caggacaaag acgtaaaatt taagatgaaa
       61 gaatatctgc cgctttgata attccaaaga gcaaaagcat ccgacagcga agtgccgtta
      121 ggttctgtga aagtggagaa ggtaaccgct aacgttgtac cggtgattcg acggagagtg
      181 tggattgctg agcttctttt ttgctctgat ggtgctgcca ggcaccgacg gacgagtaga
      241 taaaacggcc aaagaagaag ataaagctga tgagcagtac gatacgggtc atgcgactgt
      301 taaatcggtg tcgttttcgc atactggttg cctgactcac aaaaggttcc ttgaagtatg
      361 tcccacgctg tggacggtac ttacattaag gcacaacagg acaaaatggt caattcttcg
      421 ttatgtaaaa aagcgtcagt ggatacatat tttaatgtta tggaagttaa tttaattatt
      481 tacaatgatg atgtaataat gatgaattaa catgataaac gtaataattc attaatcagg
      541 gttattttgt ttgatatata tcaattggaa ctttcatatg acacttagaa tcaatgctct
      601 ctatagtgat gaataatcat cattcgaagt caggtgggat gcctgtctga atacacctcc
      661 ttcaggatgt gggggattct gctgagcatc tatgaaactg aatgcaactt atataaaaat
      721 acgtgataaa tggtgggggc ttccgctgtt cctgccttct ttaatcttgc ccattttcgc
      781 ccacattaat actttcgcgc atatttcttc cggtgaggtt tttctctttt atctgccttt
      841 ggcactgatg atcagcatga tgatgttttt cagctgggcg gcattgccag ggatcacctt
      901 agggattttt gtccgcaaat atgcagagct gggtttttac gaaacgctct cattaacggc
      961 taattttatt atcattatca ttctctgttg gggcggttac agggtcttta ctccccggcg
     1021 taacaacgtt tcacatggtg atacccgttt aatttcccag cgtatattct ggcagattgt
     1081 gtttcctgca acgctgtttc tgatactttt ccagtttgct gcatttgtag gattactggc
     1141 gagcagagaa aatctggtcg gtgtcatgcc ttttaacctc gggactttaa tcaattatca
     1201 ggccttactg gtgggtaatc tgatcggtgt cccgctgtgc tacttcatca ttcgggtagt
     1261 gcgaaatcca ttttatttac gtagctatta ttcgcaatta aaacagcagg ttgatgccaa
     1321 agtcaccaaa aaagagttcg cgctctggct actggcatta ggtgctttat tgttgctgtt
     1381 atgcatgccg ttaaatgaaa aaagcacaat ttttagcacc aattatacct tgtcattatt
     1441 gctgcccctg atgatgtggg gagcgatgcg ctatggttat aagctgattt cgctgctctg
     1501 ggcggtcgtg ttgatgatca gcatccacag ctatcaaaat tacattccca tttatcctgg
     1561 ctataccacg cagctgacca taacctcctc cagttatctg gtattctctt ttattgtcaa
     1621 ttatatggct gtactggcaa ctcgtcagcg agcggtagtc agacgcattc agcggcttgc
     1681 gtatgtggac ccggtggttc atctgccaaa tgttcgcgcc ctgaatcgcg cgttgcgtga
     1741 tgccccctgg tctgcgcttt gttatttacg catccctggc atggaaatgc tggttaagaa
     1801 ctatggcatc atgctgcgga ttcaatacaa gcaaaaactt tctcactggc tgtcaccctt
     1861 gctggaaccg ggtgaagatg tttatcagct ttcgggtaac gatctcgcgc tgcggctgaa
     1921 tacagaatcg caccaggagc gcattaccgc actggatagc catctcaagc aatttcgttt
     1981 cttttgggat ggcatgccga tgcaaccgca gattggcgtc agttactgct atgtgcgctc
     2041 gccagtgaat catatctacc tgctgctggg agagctaaat acggtcgccg aactttccat
     2101 cgtgaccaac gccccggaaa atatgcagcg tcgcggggca atgtatttgc aacgcgaatt
     2161 gaaagataaa gtcgcgatga tgaatcgact acagcaggcg ctggaacaca accatttttt
     2221 cctgatggcc cagccgatta ccggtatgcg tggtgatgtt taccatgaaa ttcttctgcg
     2281 catgaaaggt gagaatgatg aactgatcgg ccccgatagc ttcttgccgg tcgcgcacga
     2341 atttggttta tcgtcgagta tcgacatgtg ggtcattgag catacgctgc aatttatggc
     2401 tgaaaacaga gcgaagatgc ccgctcaccg ttttgctatt aatctgtctc caacctcggt
     2461 atgtcaggct cgttttcctg ttgaagtcag tcagttgctg gataaatatc agattgaagc
     2521 gtggcaactt atttttgaag tcaccgaaag taatgctctg accaatgtta agcaggcgca
     2581 aatcaccttg cagcatcttc aggaattagg ctgccagatt gcgattgatg atttcggcac
     2641 cggctacgcc agctatgcgc ggcttaaaaa tgtgaatgcc gatctgctta aaattgacgg
     2701 cagttttatc cgcaatattg tgtcaaatag tctggattat cagatagtgg catcgatttg
     2761 ccacctggcg cgaatgaaga aaatgcgggt agtggcagag tacgttgaaa acgaagagat
     2821 ccgcgaggcg gtgctctctt tggggatcga ttatatgcag ggttatctta ttggtaagcc
     2881 gcaaccgtta attgatacgc tgaatgaaat cgaacccatt cgcgaaagtg cctgaataat
     2941 gcgggccgac atttctcgtc ggcccgcaaa gcattaagcg gcgatttcag gtgtactttc
     3001 ttcttcaatt ttcaaccgcc agccagccac gccttcccag tattcttgct ccttttccag
     3061 atcaagcagt accagcgcat tctgactaaa ccagtcatgc gggaaacgca gtgtccagtg
     3121 actgtcatca gtaatcagtg tcaatgttgg cggtgtggtg gttgcctgac gttgattgtt
     3181 tagtaatacg ccaaggcgca atagctgtat cagtggcagg aactgtttct tcttaaacaa
     3241 ggtaaagcgc ggcagatcgt cgagcttaat cgctttacgg tgatagcgca ccagtgtcgc
     3301 catcatcagc tgctgttcct gattaaaacc cggcaagtca ctgttttgca gaatataagc
     3361 ggagtggcga tgcaaaccgc tgtggttgat attcaacccg acctcatgca gcatggcggc
     3421 ccatcgcagt agcgcctcca gttgcggatg cgccagcttc ggttgctgtt cccgccactg
     3481 ttcgtacatt tgcatagtgg tatccagcac tcgtcgggcc tgttcgctgt cgatgtgata
     3541 ctggttggcg aggctgctgg cggtgcgact acgcacatcc tgatgacgga aacgtccttc
     3601 catttcatac agtacgcctt cgcgtaacgc cccgtcagaa aggcgcagtt cacggatggc
     3661 taaagcatca aacacaccac ataaaatcgc cagtccagga acgaagactg ttttccgctc
     3721 ttcggaaaga cccggtaaac tcagcgatgc gaaattacgg tgacgtaaaa cttcttttac
     3781 cagtttttcc agacgttccg gggtaattat cccgtctttc tcgcccattt ccattaatac
     3841 ttcatgggcg gcttttatgg taccggaagc gcccattgcc acgttccagc cctgaatacg
     3901 gaattgccag gttaaagttt ccagtttttg tgctgccgcc atgcgagcgc gctgaaaatt
     3961 ctctttattg atgaccccgc cagggaaata aagctgggca aagctgacac aacccatccg
     4021 gcggctttca acgagaatag gttcgaaatt ttcaccaatc accagttccg tagatccgcc
     4081 gccaatatca ataaccagtt tgcgaccttt ttccggttgg gtatgttcca cgcccataaa
     4141 aatcagacgg gcttcttcat taccggaaat aatttcaatc gggtagggaa tgaccttttc
     4201 cgcgcgtttc agaaagtcag tggcgttcag cgcctgacgc agtgtatggg tgccaactat
     4261 acagacgctg gcaggagaaa acccttgtag ccgttcggca aacagcgaca gacagttcaa
     4321 accgcgcgtc attgcctctt cactcaacat attatctggc cccaggccgt ccgccagatg
     4381 cacccgctgt ttcaggcggc caataatctg catggcacca tctaccacac gggctatgac
     4441 catgtgaaaa ctgtttgaac caagatcgac cgcagcaaac tcctgcggac gaggggattt
     4501 atcgtgtatt ggcatagggt tattcaggtt gttcgagtga tttgatgtag tcataaatcg
     4561 ccaactgcgc ccgtactttg cggcgattgc cgcggggaac gtagcgatta ctgagttctt
     4621 tatcgatata acgtgctttg accgtatcgc tgaacaatat gtcgatgatg tccagcaccc
     4681 gctgcttcag gcgcggatcg agcagcggtg tcgccacttc aatacgataa tcaatattgc
     4741 gcgtcatcca gtcggcggaa gaaaggtaga cctttttatc gccgccattt tcaaaaatat
     4801 aaacccggtc atgttcaagg taacggtcaa caatactgat ggcacgaatg ttgtcgctaa
     4861 tgccttccag attggggatc agcgaacaca ttccgcgaac cagcagatta accggtacgc
     4921 cggagctgga ggccgcatac agacgatcaa ccaggccttt atcgacaagg ttatttagct
     4981 tcagggtgat accactgggc agcccttgct gcacgttggc gatttcgcgg tctaccattt
     5041 catataacag acggcgagag ttttgcggtg acaccattaa ataatcaaat gtcactgggc
     5101 ggtatgggtt ttcaataaag ttaaataccc gccgtacttc gttggtgata cgcgcatctg
     5161 cggtcagcaa cgaatagtca gtataaagac gcgcggtttt ttcgttaaag ttcccggtcc
     5221 cgatgtgtgc gtaacggacc acttcaccgt tttctttacg tgaaatcagg aacagtttgg
     5281 cgtgaatttt cagccccggc gcagagaaga taacgtgcac gcctgcttcg gtcaggcgct
     5341 tcgcccagtg aatgttggct tcttcgtcga aacgcgcctg taactcaacc accacagtga
     5401 ctttcttacc gttatgtgcg gcgtggatca tcgagtcgat gatgcgtgaa tctttcgcca
     5461 cgcggtaaat gttaatttta atcgccagta cgctcgggtc gaacgaagcc tgacgcagca
     5521 gttccagcac atgctcaaag gtgtgataag gataatagag caacacatcg cgttcgcgaa
     5581 tggcatcaaa accattgcgg aactgggctt tatcaaacca aatgtggcgt aaacgcggca
     5641 gtggtttgtt caccagattg gctttgccga cattggggaa attaataaag tctttaaaat
     5701 tatgataacg accgccgggg acgatggagt cgtagcggga aatagtcagt ttttcgcgta
     5761 acacttcaac cagcgcattg ggcatatcgc gctgataaac aaaacgcacc ggctcagcag
     5821 ttaaacgctg cttgagactg gaagacatca actccatcag gctggcttcc atctcatgca
     5881 ctaaatcgta ttcggcatcg cgtgtcatct tcattgaata ggcattcagc gcgtcataat
     5941 caaagaagcc tttgaaaata tcatcaaggc agtaacgcag aatgttatcc agaagaatca
     6001 tcggcttgcg tcgacgcggc gcttctggcg gtaaattcac aaagcgcggc actttatctg
     6061 atgggatctc cagcagcgcg taacggatgg tatcaccacg gataatttcc accgccagat
     6121 aggtgtaatc atccttcagg aactgcacta agtcagtgtc aggattgatt aaaatcggcg
     6181 taatgtgctg acgcagatac tgcttaaaat aatgacgaag ccagttttgt tgattgacgg
     6241 agagctggcg ttcattaatc aggaagatct ggttgcgcgc catctccagc aatagctcgt
     6301 tgtagaggcc gtcgaattcc tgatcggctt tcagcacccg ggactgaatt ttgcccagta
     6361 aatggcggga atgagagttg gagccttgtt cttcgctaat aatgatgcgt cgcttcagtt
     6421 cagcgaagcg gactttatag aactcatcaa ggttattgga atagatcccc aggaaacgca
     6481 tcctttcaat cagcgggtta gatttgtccg ccgcttcctg aagcacgcgt tcattgaacg
     6541 ataaccaact gagctctttt tcgatgtata gcttttcctg acccattacc acttttactc
     6601 cgttttattc acgggacact gcctggatat tatggcgagc aatattggca gatgtccaac
     6661 tatgccagaa aagggggaaa aaaactgctg gagcttgcga tttacaggtg aattgaaaaa
     6721 cgccagcggc agagctggcg ctttaactac ggggcattac tcgtcggcag cgtagccctg
     6781 cggcggcaga cgttgaccat ccagccacgc ggcgttttcg tgcattttca gacgaccatc
     6841 ggcaaaccag ctaatcacca gtggataaat agcgtgttcc tgggtttgca cgcgggcggt
     6901 gatgtcatct tccgtatcac cagcaaatac cgggactttc gcctgtaaaa taaccgggcc
     6961 accgtccagt tcatcggtga cgaaatgcac cgatgtaccg tgctcttcat caccattttc
     7021 cagcgcttga cgatgggtgt gtaatccggg atatttcggc agcagagaag ggtgaatgtt
     7081 cagcaaacgc ccggcatagt gggagacaaa cgccgggctg agaatgcgca taaaaccagc
     7141 cagcacgacc acatcgggtg cgtacatgtc gatttcatga atcaactccc ggtcataggc
     7201 ttcacgactg tcaaacgcgc tggcgatgag cgtatgcgtt gcaatacccg cctggcgggc
     7261 gcgttcaagg ccgaacgcgt cggccttatt gctgaaaact gcccgtacgg tgcctttaat
     7321 tttgttggtt ttacaggcgt caataattgc ctgtaaatta cttccgttac cggaaataag
     7381 caccacaata ttcattattc gataaccacg cgttgttcgg aatcagaggc tttgatgata
     7441 ccgattttcc acgcgttttc accgttggca ttgagcaggg cgagggcttt gtccacttcc
     7501 ggagcaggca gggcaataat catcccgacg ccgcagttga aggtgcgata catttcatgg
     7561 cgctcaacgt tacctgccgt ttgcagccag ttgaacactt ccggccactg ccaggaagat
     7621 tcatcaatca ctgcctgagt attatctggc aatacgcgtg gaatgttttc ccagaagccg
     7681 ccgccggtca ggtgcgcaat ggcatgcaca tcgacctttt caatcaactc cagcactgac
     7741 ttcacgtaaa tgcgggtcgg tgccagcaga taatcggcta atggcttacc atcaagttgg
     7801 gtggtttgcg gatcacaacc gctgacttca agaattttgc gcaccagcga atagccgttc
     7861 gagtgcggac cgctggaacc gagtgcaatc agcacatcgc cgtcgctgac tttagagccg
     7921 tcgatgattt ctgatttttc taccacgcca acgcagaaac ccgcgacatc gtaatcctca
     7981 ccgtgataca tccccggcat ttctgccgtt tcgccaccca ccagtgaaca gcccgattgc
     8041 agacatcctt ccgcgatacc gctgatcacc gctgaagcgg tatcaacatc cagttttccg
     8101 gttgcgtaat agtcgaggaa aaacagcggc tctgcacctt gcaccaccag gtcattaacg
     8161 cacatggcga ccagatcaat accaatggtg tcgtgacgtt ttaagtccat tgccagacgc
     8221 agcttggtac ctacgccgtc agtgccggaa accagcacgg gttcacgata tttttgcggc
     8281 aatgcacaca gcgcaccgaa gccgcccaga ccgcccatca cttccggacg acgcgttttc
     8341 ttcactacgc ctttgattct tccaaccaga gcattacccg cgtcaatatc aacaccggca
     8401 tctttgtagc taagagaggt tttatcggtc actgcttggg tccccacgcg ttacttgcgg
     8461 tagaaaaata aaattcggcg caattctaac agggaaagca aacgtttgcg agactgcttt
     8521 acacaacctt tttgcacgtc ttttgcccag gcgcgcggcg aaagaagact tgtaccaggg
     8581 taaaggttag ttttcggatg gaataatctt ctttcataac catttgaata taaaataact
     8641 ttatctcaag ccgttatcat tttgactaaa gtcaacgaaa agaatattgc cgccttgaag
     8701 aaaggaggta taatccgtcg attttttttg tggctgcccc tcaaaggaga aagagtatga
     8761 agatcgtgga agtcaaacac ccactcgtca aacacaagct gggactgatg cgtgagcaag
     8821 atatcagcac caagcgcttt cgcgaactcg cttccgaagt gggtagcctg ctgacttacg
     8881 aagcgaccgc cgacctcgaa acggaaaaag taactatcga aggctggaac ggcccggtag
     8941 aaatcgacca gatcaaaggt aagaaaatta ccgttgtgcc aattctgcgt gccggtctgg
     9001 ggatgatgga cggtgtgctg gaaaatgttc cgagcgcgcg catcagcgtt gtcggtatgt
     9061 accgtaatga agaaacgctg gagccggtac cgtacttcca gaaactggtt tctaacatcg
     9121 atgagcgtat ggcgctgatc gttgacccaa tgctggcaac cggtggttcc gttatcgcga
     9181 ccatcgacct gctgaaaaaa gcgggctgca gcagcatcaa agttctggtg ctggtagctg
     9241 cgccagaagg tatcgctgcg ctggaaaaag cgcacccgga cgtcgaactg tataccgcat
     9301 cgattgatca gggactgaac gagcacggat acattattcc gggcctcggc gatgccggtg
     9361 acaaaatctt tggtacgaaa taaagaataa aaataattaa agccgacttt aagagtcggc
     9421 ttttttttga gtaaagcgcc tataacacat aatacagagg ataatactat gacgcgccgt
     9481 gctatcgggg tgagtgaaag accgccactt ttacagacaa tcccgcttag tttgcaacat
     9541 ttgttcgcca tgtttggtgc aaccgtcctg gtgcccgtct tatttcatat taacccggcg
     9601 actgtactgt tatttaacgg tattggaacg ctgctgtatc tcttcatctg taaagggaaa
     9661 attccggctt atcttggttc cagctttgcc tttatttcac cggtattgtt actgttgccg
     9721 ttagggtatg aagtcgcgct gggcggcttt attatgtgcg gcgtgctgtt ctgcctggtt
     9781 tcttttatcg tgaagaaagc ggggaccggc tggctggacg tgctgtttcc acctgcggca
     9841 atgggcgcaa tcgttgccgt catcggtctg gagctggcgg gcgtagctgc cggtatggcg
     9901 ggtttactcc cggctgaagg gcaaacgcca gactccaaaa ccatcatcat ctctattacc
     9961 accctggcgg tcacggtttt aggttccgtg ctgtttcgtg gtttcctggc aattatcccg
    10021 attttaattg gcgtgctggt ggggtacgcg ctctctttcg caatgggaat tgtcgatacc
    10081 acgccgatta ttaatgctca ctggtttgcg ctgccaaccc tctatacgcc gcgcttcgag
    10141 tggtttgcca ttctgactat tctgccagcg gcgttagtgg ttattgccga acacgtaggg
    10201 cacctggtag taacggctaa tatcgtcaaa aaagatctgc tgcgcgatcc aggtctgcac
    10261 cattcgatgt ttgctaatgg cttgtcgacc gtgatttccg gcttctttgg ctctacgcca
    10321 aataccactt acggagaaaa cattggcgtg atggcgatca cccgtgttta cagtacctgg
    10381 gttatcggcg gggcggcgat tttcgctatc ctgctttcct gcgtcggtaa actggctgcc
    10441 gctatccaga tgatcccatt gccggtgatg ggcggcgttt cgctgctgct ttatggtgtc
    10501 atcggtgctt ccggtattcg tgttttgatc gaatcgaaag tggattacaa caaagcacag
    10561 aacctgatcc tgacttccgt gattttgatc atcggcgtca gtggcgcgaa ggtaaacatc
    10621 ggcgcggcgg agttaaaagg tatggcgctg gcgaccatcg tcggtatcgg cttaagtttg
    10681 atcttcaaac tgatcagtgt gctgcgccct gaagaagtgg tgctggacgc agaagacgcg
    10741 gatataacag acaaataatg taccgaccgg gcagtgttgc tgcccggttc aaacatcatg
    10801 ggattctgtg gtaaacttct cgcgattttg tgaaatcctg gttgaggtat ctctgaacac
    10861 accggcacag ctctctttgc cactttatct tcctgacgac gaaacctttg caagtttctg
    10921 gccgggggat aactcctctt tactggctgc gctgcaaaac gtgctgcgtc aggaacatag
    10981 cggttacatc tatctctggg cacgcgaagg cgcggggcgc agccatctgc tgcacgcggc
    11041 ttgcgcggaa ttgtcgcagc gtggcgatgc ggtgggctat gtcccgctgg ataaacgcac
    11101 ctggtttgtt ccggaagtgc tcgacggtat ggagcatttg tcgctggtct gtatcgacaa
    11161 cattgagtgt attgcaggcg atgagttgtg ggagatggcg attttcgatc tctacaatcg
    11221 aattctggaa tcgggcaaaa cacgattgtt gatcaccggc gatcgtccac cgcggcagtt
    11281 gaatctggga ttaccggatc tcgcgtcgcg actcgactgg gggcagatct acaaattgca
    11341 gccactttct gatgaagata agttgcaggc gctacagtta cgcgcgcgtt tgcgtggttt
    11401 tgaactgccg gaagatgtgg ggcgtttctt gctgaagcgg ctcgacagag aaatgcgcac
    11461 gctatttatg acgttggatc agttggatcg tgcgtcgatt accgcgcaac gtaagctgac
    11521 cattccgttt gtgaaagaaa ttctgaagtt gtagataagg tgtttattgt cggatgcggc
    11581 gcgaacgcct tatccgaact acgcgaagtt aatccatagt attcgtaggc cggataagac
    11641 gtggcaagcg tcgcatccgg caatgtgctt caactagcga tggagcacat cttaacccac
    11701 gatttccagt acctgctccg gtggacgacc aatccgcgct ttgccattcg cgaccacaat
    11761 cgggcgctcc attagcttcg gattatccac cattgcctga atcagcgcct cttcgctaag
    11821 cgaactgtca gccaggttca gttctttata aagatcctct ttctggcgca tcaattcacg
    11881 ggcgctattc atccccagca ttttcagcaa atcgcgcagc gttgccgcat cggcgggtgt
    11941 ctcaaggtag agtaccactt ctggctccac gccgttttct ttcagcaggt tcagcgtttc
    12001 ccggctcttt gaacagcgtg ggttatggta gatttttacc tgtttggtca ttgttcttcc
    12061 tttaatgcga attacatctt ggtataaggc ttaaagcgtt cctgcagctg gcgcaactgg
    12121 tcgatgcgcg catcgtaacg cgcttgttgc aggctgccta atttcacctg cgaactggcg
    12181 ctactcaaca gcgaaatggc ctgatcgagt cgtccggcga gcgcataacc ttctgctcgc
    12241 gcagccagct cctgatcgcg gttatttagc gcggcttccg cctgtgccag taaatcccag
    12301 ccgttgctgt catctttatt attaaaggtg tagcgattca gaatattggc cgcttcttgt
    12361 ggttgaccgc cttgtagata agcgttcgcc aggttgagct gcaacaccgg attggtgcgc
    12421 aaatcacggg catttttcag gcgattgatt gcctcattgg ctttgttttg cccaagatcg
    12481 atatcggtag ccagatcgag ataccatgcg ttgccaggtt ctgccgccag taacggttgc
    12541 agcgttttac gcgcctcgtc gtatttattg gcttccatcg cctgtaaagc acgaccatat
    12601 tgcgccgctc gttgctgacg aacgtttcct ttcgcccatt catccagcaa atcactggtg
    12661 agctggttac gtccggaatt atacatcccc agtgtgcgcg ctttcgccag atagaaatct
    12721 tccgacgact gcaccaccat cggtcgcatc tgattagcac ggttgcgggc atctgccaga
    12781 cgactttccg gcaacgggtg agtcaataaa atttccggcg ggcgcgagga gtaacgcgcc
    12841 tgatcgagta atttttcgag gaaggttggc atcgcctgcg gatcgaatcc cgagcgttgc
    12901 agcacctgaa taccaatgcg gtccgcttcc tgttcatttt gctgggtgaa actgatcatt
    12961 ccctgacgtg ttcccgccag tgtaccggtc agcgccgcca tccccgcctg cggactggcc
    13021 atcgccagta aaatagaacc taacgcgccg acccaggtca gcggcgcgct gcgttgttga
    13081 tcttccatcg ctcgcgccag gtgacgttgg gtgacgtggg agatttcgtg cgccataact
    13141 gaagccagtt gactttcgtt atcggaataa cggaacaggg cagagtgcag caccacgttg
    13201 ccgccaaaga aagcaaaggc gttaatttcg tcgttgttga tcagaaaaaa atgaaacggt
    13261 gtcttaaccg aattggcatg cgaaaccaga cgcatcccca gcgaattaat atattgcgtt
    13321 aacagcgggt cattaattaa cggcgcgctg ccgcgtagct ggcggacata atagtcgccc
    13381 atctgcattt cctgaccaat ggaaagcgtg cttcctgcgg aggttcccat atccggcaag
    13441 gtgtctgcgc tgtcggcaaa cgccggggct acctgaccaa tggtcatagc agcaatgagg
    13501 gttgcaacca ggtttttttt caactgcctg aacataacct ctatcctgta tttctgaaga
    13561 tagtcatttg accgatactg ttaaatatcg ttcccaccgc gccgcctttg agtgtaacgg
    13621 cgaagattag cccactcaaa tgacttttca gtttcctgaa tccgtcataa aaagcgaagt
    13681 cttttttgtg acatttcgat acaattgcgg atcgcaatcc cgctcttgag gttcagggaa
    13741 ggtttttatg ctcgaaatgt tgatgcaatg gtatcgccgc cgttttagcg acccggaagc
    13801 gattgccttg ctggttattt tagttgccgg atttggcatt atctttttct ttagtggcct
    13861 gcttgctccg ttgctggtgg ctattgtgct ggcctatttg ctggaatggc caaccgtgcg
    13921 cctgcaatct attggctgct cccgccgctg ggcgacgtcg attgtattgg tggtttttgt
    13981 cggtatattg ctactgatgg cgttcgtggt actgcctatc gcctggcaac agggcatcta
    14041 cttaatccgc gatatgccgg ggatgctcaa taagctttct gactttgccg ccacgttgcc
    14101 gcgccgctat ccggcgttaa tggacgcggg cattattgat gcaatggccg aaaatatgcg
    14161 cagtcggatg ctgaccatgg gcgattcggt ggtgaaaatt tccctcgcct cgctggtcgg
    14221 tttgctgacc attgccgtct atctggtgct ggtgccattg atggtcttct tcctgctgaa
    14281 agacaaagag cagatgctga acgccgttcg tcgggtgctg ccgcgtaacc gtgggctggc
    14341 aggacaggtg tggaaggaga tgaatcaaca aatcaccaac tatatccgcg gcaaagtgct
    14401 ggagatgatc gtggtaggga tcgccacctg gctggggttc ttgctcttcg ggctgaacta
    14461 ttcgctgctg ctggcggtgc tggtcggctt ctcggttctt attccgtaca ttggcgcatt
    14521 tgtggtgacc attccggtgg ttggcgtggc gctattccag tttggcgcag gcacggaatt
    14581 ctggagctgc ttcgcggtgt atctgattat tcaggcgctg gacggcaacc tgctagtacc
    14641 ggtgttgttc tccgaagcgg ttaacctgca tccgctggtg attattttat cggtggtgat
    14701 cttcggtggt ttgtggggat tctggggcgt attcttcgcc attccattgg cgacgctgat
    14761 caaagccgtg atccacgcct ggcccgatgg gcaaatcgcg caagaataat cttcagcccg
    14821 acgaactgtt tcgccgggct gatttttcag gattcctgac gtaaataaat agcccgataa
    14881 cacatactca ccagcactgc accgccgata atattaccca gcatcaccgg gagcagattg
    14941 gcggtaataa aatggctgac cgtcagtgcc ggaaaattgt ctgcgctact gtgcgccagt
    15001 tgccagaagg aggtaggggc gaaatggcga atggcaatgg cgaacggaat cacaaacaaa
    15061 ttggcgatac agtgctcaaa gccactggcg acaaacaggg tgatgggcaa aatcatggcg
    15121 acgattttgt cgcataacga acgcccgcaa taactcatcc acagcgccag gcaaaccatc
    15181 agattgcaca taatgccgag gctgacagat tcagtaaatg tatgatgcat tttgccctcg
    15241 gcgcagtgta aaaccgcgac tccccacatc gcgttttcac tcatcaccag cccggaaaac
    15301 cagattaaca aactgaaaca ggcaatacct gccagattac cgcaggccac cagaagtgcg
    15361 ttaatcagcc aagttcgcca actaataacg cccctacttt ttgccattac cgtcattacc
    15421 gacgaggtga acaggttggt gccgcaaacc gccagcaaaa taaagccaag tgtaaagcaa
    15481 aggccgccca ccagatgggt taatgcctgt gaagacgggg catcggcgat aactgaaagg
    15541 taaaaagtaa atccgatcgc cataaacacg ccagcggaca tcgccattaa aaagaccgac
    15601 actttactgc gggcaatttt atgggcggca atccgttcag cgattgccgc ttttctggcg
    15661 ctcaactgca agtcgaaaga gagtttgttt ctcatgcata ctcctgcaaa agcagattac
    15721 aacacctcgc gaaccgagat tcccagacgc tgcattcgtg acagcagcgt ggtgcgcttc
    15781 atccctaatc gtgtcgccgc gccacggggc ccggcaacaa tgccattggt ttctcgcaat
    15841 acctgaacaa tgcgctgacg ttcttcttcg tcattctctg gcgtcgtcgg gtgcagcaac
    15901 tgcgccattg aactgcgaag cgctgaatct tcatttagcg tcggcagtaa acggctttgt
    15961 cggacattaa gatgtaaatt cagactgtta ccgcgagtca gcagtaccgc tcgctcaatc
    16021 acgttttcca gctcgcgcac gttgcccggc cagtcccacg acatcaactg gcgtagcgcc
    16081 tcggtcggga tggcgtcaat tgaacgattc atatggcgcg ccattttttg cgtgaaatgc
    16141 tttgctaaaa gagggatatc ttccggacgg tcgcgcagcg gcggcaattc cagtgggaag
    16201 acattcaggc gataaaagag atcgctgcga aactggcgat cttcaaccat ttgccacaaa
    16261 tcacggttgg tggcggcaat gactctgaca tttaccggga tcgttctact cccgccgaga
    16321 cgctcaatct cccgttcctg caatacgcgc agcagtttag gctgaagttc taacggcaga
    16381 tcgccaattt catcgagaaa caacgtgccg ccatcggcaa tttcaaaacg accacgatgg
    16441 gtattaatcg caccagtaaa cgcccctttg tcatgaccga ataactcact ttccagaaga
    16501 ctggcgggga tggcagcgca gttgattttg accagcggct tgtcgcgtcg cgggctaagt
    16561 tgatggatcg ctctggcgat cacctctttg ccggttccgg tttcaccgca aatcaacacc
    16621 gtactgtcgc tcttcgccac aatatctacc tgctggagta ggtcttccat tgcctggctt
    16681 tgatagataa tgtcaccgat gcccagattc gaaaggagct gctcgctaag ctggtggttt
    16741 tcttgctgca aactttcctg caaatcggtc atgctacgcc aggcatcggc attgccaacg
    16801 gcaatagcga tgcgatcggc tatgtgttgt agtagctggc agttttcctc actaaagaga
    16861 gtggaagagg tatgcgccag caacaatgca cccggtgtat ggttgccaaa ggtaagcggt
    16921 ataaggagcg cagattcgca gccgttagat gcaagtaaga gtaataacgg gtcgcgttgc
    16981 cagagaaaca gatcgtctgc acggtgcgtc aaggtcggtt gtcgggtttg tagcgtttgt
    17041 gtcagcaata cactgtcgcc aggcatattg cgtggcagac acgcacaatg tgaggcagaa
    17101 agatcgctgc accacaggct aaacttctcg ttctttcgat gatcgcccag taccatactg
    17161 actgaagcca gaccgaaaaa atgatggatc tcacgagcga cgtcagcgat cagatcgtcg
    17221 agatcaagat gtgacagcac tgcattagta atatcaacca gtacccgatg atgatcgcga
    17281 tctttctcca gcaaacgcag cgccacctgc tgttcttcat cattgagcca tgattgcaga
    17341 accggggtga ttatccgcgc cagccagtta aacagtgtgc aatcctgggg agtaaaacgg
    17401 ctgccgtggg tacgaataaa gcgtagctcc gccattccac tactgccgag aaggacggcg
    17461 tgacgcgaat aatcgaccat ctcgccctga tactctgttg ttgccgtcac atcgctgcca
    17521 tcatcatggt agcggtaata gaagttgctt ttgagaacat gcagttcaat aagttgtacc
    17581 gggctgaacg gcggcggcat cgggatgaag gcgcgtaata aagacgcctt accgtgtttc
    17641 tgtgctaacc gctccgcgag cattccgttt accgcttcaa ttgttattcc ttgtggcggg
    17701 gcaaacatcg cctcgtctga catagccatc aacagtcctt attaatgaga aatccttctc
    17761 acgcaatttt tctggccatc agatagatgg ccggttcctg cacaatttca tccaacagat
    17821 gaatgaattg aatgctacgc gcctgccaca cagacgtcgc gtcatccgcc agtggcgcga
    17881 atgcccgggt caacagggcg gtgtggctgg cgtcaataac aatctcacca aaagtcatca
    17941 cccccagcat cttacgtcgg gcttgctcct cttcgacctg ggcaagccaa tcttcgtatt
    18001 ccgtcagtgg gcaggggaag gcgacattca gacaatcaat cacgccaacg tgatgaccga
    18061 tagccagcga gtaatacatt acctgagagc tgtgttccgg catctcgtcg ctactggcga
    18121 caaacttttt tcgcagcgtc cagaaaacaa tttccccgca ctcttcagtc atgtaacccc
    18181 cggagtacgc gaaagagttg ctgaacgata ctattgagac gtggatcgtc ggcatcgcgc
    18241 aaccaggtgt taacccgatt tcctgtagga tcgtcgctta aatggcgcag gagccgatcg
    18301 caaatttctc gcccctgacg atagcccgcc agccgccgcg cttctcgctc aatggcgata
    18361 cgctgtgatg gcgggatctg cggccacaac ggttgcatag tcaccccggt gggatcgcga
    18421 taatccaccg cgtgaatctt ctgttgcagc aaaccgagcg ccacggcgaa accgtgaatg
    18481 gtggcggccg gtgttggcgg gcagccgggg atccaaacat caatggggac aatggtgtcg
    18541 ctgccgcccc agacgctgta gagatcgtgg aaaatccgcc gccggcaccg cacgcgccgt
    18601 aggaaacaca gattttatga tcgggggcag actcatacgc ccgaagtgca ggcatacgca
    18661 tcgcccgggt gactgcgcct gtaaataaca aaatatcggc gtgacgcggt gatgaaataa
    18721 ccttaatgcc aaaacgttct gcgtcgaata ctggtgtgat ggcggcaaaa atttcgattt
    18781 cacaggcgtt gcagccgccg caatcgacgc gataaacgta agccgagcga cggatatcct
    18841 gtagcaaatg ctgcttcatc ttttgcgttt gctcgtccag cgtgatgggc tggctgacat
    18901 gttgtgtaag cactggactc atagctgctc cttagccacc agcggtacat ctgtatcgtc
    18961 gttgagcagc gtcgcgcgtt gtttgcactc cgggcagacg ctcgcttgcg cccataacat
    19021 ttcgcggttt tgtggcgcat tttgttgctg tgccaacaat tcagcagcca gtgcgacggt
    19081 tttttgcggg gcaagcgggc gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg
    19141 ggtatagaga tcggctttat tggtgacggt cagttcaaag ttattggtaa gctggatggc
    19201 tctggtcggg cacacttctt cacaacgtcc gcagtaaata caacgcctca gatagagctg
    19261 ccaggtgcgc gaattttgct ggtcgtcggt ctggatagtc agcgcatttg ccggacaagc
    19321 acaggcgcag gcaccgcagg caatacattg gctgggcatc aggtccggtt tcccgcgaaa
    19381 gcctgggctg acctccagtg gcgcgaaggg atatttcacc gtcgcggttc cggcgcgcat
    19441 aatagttttc agtaacttca gcatcttctg tccttacttc agcggcgaac ggttacgatc
    19501 aataccgtag cgttcgatct ctttatacgg cacggttttt gactggcgct tgcgcacatc
    19561 taccagcgtc acacggtcgg tacaggagta gcagggatca aggctaccga taatcagcgg
    19621 tgcgtcagaa acggtattgc cgcgcagcat gtaacgcaac accggccagt tggcgtaggt
    19681 ggcggcacgg cagcgccagc ggaacaattt ttggttatca ccgagcatgc tccagtgcac
    19741 gtcttcaccg cgtggcgctt caacaaagcc cagcgcgaat gcgtgaggtt tatagctaaa
    19801 gccttcggtc agcagtgggg tatccggcat gttgtcgagg gcaaattcca gcattgccag
    19861 cgaatcaaac gtctctttga cacggaccat cacgcgggag aaaacatcgc cgccggtaaa
    19921 ggtaaacagt gtttttggaa tattgccgta gtcggcgtag gggtgatcaa agcgcaaatc
    19981 acgggcgatt tgtcggtcga gaatgccaat gccctgagtg cgttgttcca tattcggcgt
    20041 agcaagcagc atctctacca gctccgacac gtcggcgcgc atctcgcgca ccagtttcag
    20101 cgtttgcaga cgttgctctt tgagaatatc gcggcgaaca ccaccaatca gattcagacc
    20161 gtaggtttta cgcgacccga tcagcaattc cgccatcgtc atcgactttt cccgcacgcg
    20221 gaaaaattgc ataaagccgg tatcaaaacc aacgaaatgg caggagaggc caaggttaag
    20281 caaatgactg tgtagccgtt cgacttccag cagaatcgag cgaatagtat gtgctcgttg
    20341 cggcacctca atccccagtg cattttcaac cgagttggta taggccacac tgtgggcaaa
    20401 accgcaaatc ccacacacgc ggtccgataa gaaggtcact tcgttgtagc ccatccgcgt
    20461 ttctgccagt ttctccatgc cgcgatggac ataaaacagg cggtaatcag catcgacaat
    20521 ttgctcgcca tccacaaaca agcggaagtg acccagttca tcggaggtga tatgcagcgg
    20581 gccgacaggg atcacctgcg catcgctgtt gccctcattg ataaacggat acgtttcgga
    20641 atcagtcgtc ggttcagggc gcagtcgata atccatcgca tctttgcgca gcggatgcat
    20701 atcttccggc cagtcatcgg gcaacaccag gcgacgatga tccggcaggc caaccggaat
    20761 cagcccgtac atatcgcgaa tttctcgctc gccccagacc gcggcaggga cgcgcggtgt
    20821 gactgacgga aactcccgac tgtcggcatc gaccagtgcc ttcaccacga tccagcattt
    20881 ttcggcccct tccattgaaa gggcataata aaccgcgtaa tgaccgttaa gtgtccgctc
    20941 gtcgttgcca aacaggactg gaagccagcc atcatgttga taataaagat aatgtacaac
    21001 gtcaggcagc agattgattt tcaccgtaat ggtgacctgt tcaggcgttt gtcgctcttc
    21061 atccagcacc gcgccgggga actgcgtttt cagggcggcg agaatcgctt cgccacgatt
    21121 tgacgatgaa ttaacgttca ctgtgtttct ccgaaacaga tgcggtgcct gaaggcaaaa
    21181 aatcatgcca ggtgctacgt tgtgcaggca ggtcgtgcgt ccctgagagg actatagtgg
    21241 aagcgcccgc caggatcctg atgacaggtt gtggaatatg cgttcccatc gccagcatca
    21301 tgaccagcag aatcaccatt ggcgaggtgg tcaaccagcc gagatcaccc cggttaacgg
    21361 cctgcggcgg tttcgccatt aacacccgcg ctgccatccg taccaggccc gccagcacca
    21421 gcgttaacag caataacagc aggacgataa tcagcaggtg attacgtgcc agtccggcgg
    21481 taatggtcat aaattcgcta agaaaaatgt tgaagggcgg catccctgcc agcgccagcg
    21541 caccgccgcc aaacagcacg gcggtaaatg gcatgatttt gagcatccca cagacgacgt
    21601 tgagatcgcg cgtgccgtac ttgagcagta cattgccgga accgcagaac agcagcgttt
    21661 ttgccagact gtggtttaag gtgtgcagca gcgcggcaaa aattcccagt ggcccgccaa
    21721 tgcctagcgc caccgcgacc agtcccatgt tctccacgct ggagtacgcc agcagacgct
    21781 taatgtcccg ctgtaccaga atgaaaaatg ccgccacggc aaccgacaac atgccgaaga
    21841 tgagcaacaa ccggttgggg aaatcgctgc cgatggcctg gcagataatg atgtaatagc
    21901 gaattagcac caacagcgcg cagttcagca atacggcgga gagcagggcg ctgaccggac
    21961 tcggtgcttc actgtgagca tccggcagcc aggcgtgcat gggaaatagc ccggttttgg
    22021 tgccaaagcc aattagcaca aacacaaagg ccaacagcat taatgttggg tcaagcaagg
    22081 acgattgctt aagaacctcg ctccagaata tcgccatttc tgcctgcggc ataacgctgg
    22141 cgacgttggc gtataccagc acggtaccga acagaccaaa agcgacacca acagtacaaa
    22201 taatgatgta cttccatgct gcttccagcg atgaacgctg accgtaaatg cctaccagaa
    22261 acgccgagct taaggtggtg gcttcgatcg ccgcccacat cacaatcagg ttattgctgg
    22321 taacaaccag cagcatggta aacaaaaaca gatggaagaa accgtagtaa tcgcacagcg
    22381 taacggggga aagctcgccg tgtgccactt catgacgcat gtagccaatc gagtaaacac
    22441 cggtgagaaa gccaatcaca ccaagaatgg cgaggaacaa accgcccaga ccatcaatat
    22501 gaagccacag tcccgcagcg aatatttctc ctgcatcagc ggcagtttgg accacccaga
    22561 gtgccagaat cagcagcagt gtgatcccta agctatgtaa tacagtcacc gtacaagtcg
    22621 cagagagccc ccgtttccgg caggcaaaac agagcagcga aaaaagcagc ggcgtgagca
    22681 ggagtaaagc gaacatcaca gaataactca tctcattatc ccttcagcgc ggtcaagttg
    22741 ttcacgtcca gcgtgccgtg ggtacgccat atttttcttg ccagtaacac catcacaatg
    22801 acggcgaaga tggcgtcggt agcgatacct atttccacca gttccggtgc tcgccaggca
    22861 agaagcgcca gcaccagatg ggagccgttt tccatcaggc agtaaccaaa aatttgccgc
    22921 aggatattgc gctggctgac aatgcacagc aggccaagca gaaaatgacc taacgctacc
    22981 gccagcgccg gtttcagccc ggtagccatc ggtagcttca cgggctgaac gacaaatgcg
    23041 caaagcagga caattaacgc cgcgagcagt gccatcattg ccggaccgaa taacgctttt
    23101 tccgggatgt tctggggaat atttcgtgca gcgtaagtca tgattaacgg taccagcagc
    23161 actttggtga taaaggcgct ggcggaccag atcagcagtt gctctgcggc gaacaggcac
    23221 gagagagtgg cgaaaataga caccagcacc agcgactggc aggcgtaaaa tccgcatgac
    23281 aggcgatagc ttttgacgct aatcacaaac agcgatgtca gcatcatcag tcccgccaga
    23341 ttatttacga tcatagaacc ggtcattcat catctcctta ctgcaaccag gtcgcggcga
    23401 ttacgctgga aatcagcgac atcacaatca gtacaatcaa caccaggcgc attgaccctg
    23461 gcagcggtgc ggcatcggcg acggcctcgc tcggtttgcc aggcacaacg cgaccaaacc
    23521 agcgaataaa ccaggcgaaa ctggcgaccg attcaatcat cagcagaatc atggcgggca
    23581 gcaggatcca gtactccact gacaacgcaa aaccggcagc aaacagcggg aatttactga
    23641 agaagccgtt gaacggcggc acgccggtaa tcgccagcgc tgccacgcag aaacccacgc
    23701 ctggcaacgg cagggtgtgc aatacgccac gcagacgcgg caacaagcgc gtgccgcagc
    23761 tgtaactcag cgcacctgct acaaggaaaa acaggctttt agcgaacgcg tggttgacga
    23821 tgtaggcgat gctgccctcc agcgccagcc gcgagccgaa gatggagagc gacaaaccga
    23881 agaacatcca gccaagttga gtgatggtcg accaggccag caaccgcttc atatcctgct
    23941 gtggcaaata catcagaaag ccataaagaa tggtgaccag cgccatgacc atgccaacgc
    24001 cgccaatcac atgcgggata ttgccgccgt cgataatggc gcgggcaaaa atgtaaacgc
    24061 ccactttcac cttcgatgcg gcgtggagat aggcactgat cggtgttggc gcttccattg
    24121 catccggtag ccacgcttgc atcggtagct gggccgattt cccccacgcg gcaaacagga
    24181 tgccgccata aaccagataa cgtgcgtcgc cgtgtaactc gctcatcgcg ctaagcgcaa
    24241 acgttccggt ctgcaaaaac agcgtggcgg cggcaagata caaccccaac gagccgatat
    24301 gagtgataag taacgctttt agtgctgaac gctgtgcttt atcgctctgg taataactga
    24361 tcaacgccca ggagcagccg cccgtaattt caaaaaacaa caactgaccg agcagcgtcg
    24421 aggagagtac cagtcccgcc atcgcgccga taaacaacag caaaaatgcg taataacgat
    24481 tcgtgccgtt atgcgggtgt tcgcgatttt tatccgtcag ataacccgtg gagtagatcg
    24541 tgaccagcaa accgagaaac accaccacaa acagaatcag cgtactcacg cgatcaatga
    24601 ccaggccaaa caatgccaca tcccccacgt tgaccaatgt caacgtgacg gcaactttat
    24661 cggcctgata aaacgcggag atcagtgaca acatgcacag cgtggtcagc gcggcgaaca
    24721 aaacccccca ttcggcggcc cgacgttgtg gcgaaaacga aacgaccagt gcgccgataa
    24781 aaggcagcaa taacgtcgtc agagcaagat tttccatatt ccgtcagtgc tccttacaga
    24841 ccggttaacc agaagaccca tgcaagcgca gcaaggctga agccaagcca ggtcacatgg
    24901 tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg cgattgaggc cagtacaaaa
    24961 atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca gcaggcaggc gagagaaagt
    25021 tcttgcgcgc gcccaaagga cagaaacagg gccacaaaca gtgatgccat cacgacctgt
    25081 ttcagcccca gcccccattt cgctagcgcc agcccggcac cggaatattc ggtcagcggg
    25141 ccttcctgta attcctgttc tgcttcagca acatcaaagg gaatttttcc catctcaatg
    25201 aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg ttagcggcga gttccagccc
    25261 atcgccagcg tattgctgat catctcgata tgcgtggaac ctgctatcag cgccaatacc
    25321 agcagtgaga gaataagcat tggttcgacc agaatgccga gcgtcaactc gcgactggca
    25381 ccgactcccg caaacggact tccggtatcc agcccggaaa gagcaaagaa aaaacgaaac
    25441 agggcaagaa gatagataag ggtgatcaga tcgccgccgc ccgcaaaagg ggaaacggta
    25501 ataaacagtg gtaaggccat cgccagcacc agcatgctgc tgattaatac ccacggcatc
    25561 aggcggaaca tcagacctga agatgtcggc gcaacttcct ggcgtttaaa cagtttgtgg
    25621 atatcgcgat aatcctgcca gatccccggc ccgcggcggg agtgcatacg cgcgcgtatc
    25681 tgccgggaaa tacccgtaaa aagtggggtt agcatcagca gaatcacggc ctgtgctaac
    25741 gcaaaaataa ccagatatcc gtcgcaaaga gtttgtctca tggtgatttc cttagacggc
    25801 aatagcgatt agcagcacaa ccagtgcggc gaccacatac aggcaataga gacgaaagtc
    25861 gccgctttgc agatgctgta tttctttggc cagccgttgg gtggcgctca cgatggggcg
    25921 gatcacccgc tcatcccaga agggttctgt gctctgagcc ctggcggtga cgtgcgcaaa
    25981 acctttattt agcctcagcg tagggtcgag ctgttgtcgt agacgaaata acgcagagaa
    26041 gaccacacgc agcggctgca tcacgccatt gcctgacggg gccatcgcat tttcccagcc
    26101 gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct gcgcggcgcg aacgacacca
    26161 cagccagtaa aggccaggca gcaaaggcat cgccagtagt aacagcaaca gtaatgaggg
    26221 ggtgacctgt gtatgaaacg tgccgggtac aagtgctatt ccgctggcga cagtgacggg
    26281 aggggtattg gtaaacgcat gggcaatatg cattatcttc ggtgccagcc aactggcact
    26341 aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg gcgacgatca tcgtatttgg
    26401 cacttcctgt gcctcttcag cgtgtgtact gcgcggcgca ccacagaaag taataccgta
    26461 catttttacg aagcacatta ctgccagccc accagtgact gccagcatta ccatagcaat
    26521 aggacccgca agttgtagcg ctacggcttc cacacgactt agtgagaaca gcgactgcca
    26581 ggtgtaccat tcgctgataa aaccattcag cggaggaatg gctgatatcg cgaggcaacc
    26641 aatcaggcat gctgcggctg tccacggcat ccgtttcgcc agtgccccca ttttttccat
    26701 gtcgtgggta tgcaaacgcg aaataatcgc gcccgcgccg agaaatagca gccctttgaa
    26761 cagcgcatgg ttgagcagat gaaacagtgc gccgagcagt ccaaccacgg tgagcagcgg
    26821 gtcgtgcagt gacagaccga ccatcgccac accgactgcc agcaaaataa tgccgacgtt
    26881 ttcgacggta ctccaggcca gcagccgttt gatatcctgt tccgccagcg catacagcac
    26941 gcccaggagc gcggagattg cgccgatcgc catcaccaga atgccccacc acagcggcaa
    27001 acccgtttgc gccagcaggt ccatcgctac tttcaggatg ccgaaaatac cgattttgac
    27061 cattacgcca gacatcaacg ccgaagcgtg cgatggtgcg gcagggtgag cgcgcggcaa
    27121 ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa ccgaaaaagg ccagcaggaa
    27181 caccgccgac gccagccccg gagaaagtga aagcgtgcgg aaactggcaa aatcgaggct
    27241 gccgctttcg cgccccatca gcaagaaggc gatcataatc agcaccgatc cggcgtgggc
    27301 gataaaaaag tagagcatgc cagcgttgat cgacgtttta tcctgcctgg caatgaccag
    27361 aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg aaccaaaaag cgttgtccat
    27421 caccagcagg gcaaccatcg atgcgatgaa aagattcata aagaagccca tcgccgccgc
    27481 gcctttgccc tcgtattcgc gcatataagt caatgaatag agcgaacaaa ccaccaccag
    27541 caaggagata accagcacca taaatgccga gaggctatcc attcgcaggg taaaatcggc
    27601 aaagtcaaaa ggggtggcaa aacgggcgac taaagttaca ccagcatgta attgcgtaat
    27661 tccgctgatg atgccaatca cgccgcccac cagcgatgtg atgccggaaa gcttaatagc
    27721 cagtctgtcc agaccgagta aaaacagcga agccagactg gcaaacagat agaggatcag
    27781 cgaccaggtt aataattgca gggcatccat tagcgttgct cctgagtgag ggaaagggga
    27841 tcctccccgc cattggcaaa ccagcttgcg gcaaggcgct gtttttcttt catctgtcgt
    27901 tgcaggctat cgtcggtgat cagccgtaac gcctgattcg ggcaagcgcg aacgcaggcc
    27961 ggaccttctg gcaagaaatc acacaggtcg catttcaccg cgacggtctg gacacccggt
    28021 tcccagcgca gcaatgcatg ttggggcggc acgttttctt cgccgtcttt gagtgagcct
    28081 tcagcctgaa aaacatattg cgcaggggca ttcaccggac ggcttcctga agcgctgatt
    28141 gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa tacagaggct ttcgttgagt
    28201 tggatcgcat catccctctg ggagatggca ttaaccgggc agacctgcag gcaaggggct
    28261 tcctcacagt gatgacacac gacaggggca gtgagtgttg acgtcttcgc cagggccagg
    28321 cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg aacaggcagc gagacaggta
    28381 ttacatcctg tacaccacag tggttcggcc accacaaagc ggttcatgtc ggctccttga
    28441 aaatgatatg aaacattgca tttcatattg cataaaggat gccattctga ttaactcaat
    28501 atattcaata agttatgtat tgactggatt ctgaacggta caaaaatgat ggtcattaac
    28561 gttcagcaaa aaatggattc gacacccgtg tcgcagggga aagcgagaat gtgatcttga
    28621 tcacgagaag tgaggtatgc tggccgcaag cgcagccagc acggaatgga gcaaagtaat
    28681 caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt gattgctggt tttgaaatcg
    28741 tcaaagacat gttcgatttt gccatcagcg tcaatcagga agctgatgcg atgaatgcca
    28801 tcgtaggttt tgcccatgaa ggacttttca ccccagacgc cgaattgttc gcacacctgg
    28861 tggtcctcat cagacaggag cgtaaagtta agcagctctt tttccgcaaa acgggagagt
    28921 ttttcgggtt tatcggtgct gatacccagc acatcaacgc ccgctttttt caactcatcc
    28981 atgttatcgc gtaagccgca ggcctgtacg gtacagccgg gggtcatggc tttcgggtag
    29041 aaataaacca gaacacgctg tccctggaag tcggtcaaat taacttgttc tccgtcttga
    29101 tccggcaagc taaatttcgg tgcgatatca ccggctttca gtggattcat tacttaactc
    29161 catcctgttc atcatgttgg gaataattga cgacgttaat actgccttgt gcattgagtt
    29221 ctgtacatag ggctttgaac gcttgctcaa tatttgctgc gtccgcagat gcggggctgt
    29281 gggcggttat ctgaatatgc aactgcgcag cccgttcatt ttcagcaggt tgcgtgcgcg
    29341 acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag tgctgtgaag cgttcaatta
    29401 aatgcgggga gtctgccaca tcgacctgaa cccagacaga tgctggcatt ggcggacgcg
    29461 gacgcgccgt cgtgcgcttc atcacgatta aaagatccag ttcggcacct ttcaacggta
    29521 acgttgattc aatcagagtg atggcattcc atgaaccgga aagcagcatg ataaacgtga
    29581 actcttctcc cagcatcgcc aggcgactgt cttcaatatt acagccgcaa ctactgacat
    29641 gacgggtgat ggtgttcaca attccagggc gatcggcacc caacgcagtg atcaccagat
    29701 aatgttgcga tgacagtgtc aaactggtta ttcctttaag gggtgagttg ttcttaagga
    29761 aagcataaaa aaaacatgca tacaacaatc agaacggttc tgtctgcttg cttttaatgc
    29821 cataccaaac gtaccattga gacacttgtt tgcacagagg atggcccatg ttcacgggaa
    29881 gtattgtcgc gattgttact ccgatggatg aaaaaggtaa tgtctgtcgg gctagcttga
    29941 aaaaactgat tgattatcat gtcgccagcg gtacttcggc gatcgtttct gttggcacca
    30001 ctggcgagtc cgctacctta aatcatgacg aacatgctga tgtggtgatg atgacgctgg
    30061 atctggctga tgggcgcatt ccggtaattg ccgggaccgg cgctaacgct actgcggaag
    30121 ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt cggctgcctg acggtaaccc
    30181 cttactacaa tcgtccgtcg caagaaggtt tgtatcagca tttcaaagcc atcgctgagc
    30241 atactgacct gccgcaaatt ctgtataatg tgccgtcccg tactggctgc gatctgctcc
    30301 cggaaacggt gggccgtctg gcgaaagtaa aaaatattat cggaatcaaa gaggcaacag
    30361 ggaacttaac gcgtgtaaac cagatcaaag agctggtttc agatgatttt gttctgctga
    30421 gcggcgatga tgcgagcgcg ctggacttca tgcaattggg cggtcatggg gttatttccg
    30481 ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg caaactggca gcagaagggc
    30541 attttgccga ggcacgcgtt attaatcagc gtctgatgcc attacacaac aaactatttg
    30601 tcgaacccaa tccaatcccg gtgaaatggg catgtaagga actgggtctt gtggcgaccg
    30661 atacgctgcg cctgccaatg acaccaatca ccgacagtgg tcgtgagacg gtcagagcgg
    30721 cgcttaagca tgccggtttg ctgtaaagtt tagggagatt tgatggctta ctctgttcaa
    30781 aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt tattactcgc tgcctgtagt
    30841 tctgactcac gctataagcg tcaggtcagt ggtgatgaag cctacctgga agcggcaccg
    30901 cttgcggagc ttcatgcccc ggctggaatg attttgccgg tgacctccgg tgattatgca
    30961 atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc tggacattcg tccaccagcc
    31021 cagccgctgg cactggtttc tggcgcgcgt acccagttca cgggcgatac cgcttcattg
    31081 ctggtggaaa atggtcgtgg caatactctg tggccgcagg tggttagcgt gctgcaggcg
    31141 aaaaactaca ccatcaccca acgtgatgat gctggtcaga cactgaccac cgattgggta
    31201 caatggaacc gtctggacga agacgagcag tatcgtggtc gttatcaaat ctctgttaag
    31261 ccgcagggtt atcagcaggc ggttacggtt aaactgctga acctggaaca ggcgggcaaa
    31321 ccggttgcag acgcggcttc catgcagcgt tacagcacgg agatgatgaa cgttatttcc
    31381 gccggtctgg ataaatctgc cactgacgcc gcgaacgctg cgcaaaatcg tgcctccacc
    31441 actatggacg tacaaagtgc agctgatgac accggtttac caatgctggt cgtacgcggg
    31501 ccgttcaatg tggtttggca acgtctgcca gcggcgctgg aaaaagtggg catgaaagtg
    31561 accgacagca cccgttcgca gggcaacatg gccgtaactt ataagccgct gtctgacagc
    31621 gactggcagg aactgggcgc gagcgatcca ggcctggcat ccggtgacta taaactgcag
    31681 gttggcgatt tagataaccg cagcagccta cagttcatcg atccgaaagg tcatactctg
    31741 actcagagtc agaacgacgc gctggtagct gtcttccagg ctgcgtttag caagtaaaaa
    31801 tacagggctg gaatcatccg gccctttttt ctgatatgat acgcaaacgt gtgcgtcggc
    31861 aggaaaacgc gattttagcg gtaattcgca cgaaatttgt ttgtcggacg tagttcggat
    31921 aaggcgttca cgccgcatcc gacaaaacat ccggcacacc agacagcaaa agattttaaa
    31981 acgttaattc acacccagga gtgataaaga tgcaaaagca agctgagttg tatcgtggta
    32041 aagcgaaaac cgtatacagc acggaaaacc cggacctgtt ggtgctcgaa ttccgcaatg
    32101 atacgtcagc aggggatggc gcgcgcattg agcagtttga tcgcaaaggt atggtgaaca
    32161 acaagttcaa ttacttcatt atgagcaaac tggctgaagc gggtatcccg actcaaatgg
    32221 agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct ggatatggtg ccggttgagt
    32281 gtgtcgtgcg taaccgtgct gctggctctc tggtgaaacg tcttggaatc gaagaaggta
    32341 ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa cgacgccatg cacgatccga
    32401 tggtcaacga atcttactgc gaaacctttg gctgggtgag caaagagaac ctggcgcgta
    32461 tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa actgttcgat gatgctggtc
    32521 tgattctggt cgacttcaag ctggagtttg gcctgtacaa aggtgaagtg gtactgggcg
    32581 atgagttctc cccggacggc agccgcctgt gggacaaaga aacactggag aaaatggaca
    32641 aagaccgttt ccgccagagc ctcggtggcc tgatcgaagc ctatgaagcc gtcgcccgcc
    32701 gcctgggtgt acagctggac tgatttttct ggttgcttgt ttctttagcc ggagggtact
    32761 ccggctaaat gtctttgata cccccgctac aatacttctc attatctccc tttgacagga
    32821 aagtcatacc aggccaatat gtatatacat aacgtatatc ccaaaaaggg ggggctatgg
    32881 aaactactgt atttttaagt aatcgcagtc aggccgtcag attaccgaaa gcggtcgctc
    32941 tgccagaaga cgttaagaaa gtagaaatta ttgcgattgg tcgtacaaga atcatcactc
    33001 ccgccggtga gtcctgggac agttggtttg atggcgaaaa tgtgagtgcg gattttatgg
    33061 atatccgtga tcaacccgca atgcaggaaa gggaatcatt ttgatgctga aattcatgct
    33121 ggataccaat atctgtattt tcactattaa gaataaacca gtacatgtca gagagcggtt
    33181 taacctgaat tctggccgga tgtgtataag ttctgtaaca ttgatggagc ttatctatgg
    33241 ggcagaaaaa agtcagatgc cagaacgcaa tttggcggtt attgaaggtt tcgtatctcg
    33301 tttggtagtg cttgattatg atagtgctgc tgcgacgcat accgggcaaa tcagggccga
    33361 gcttgcccgg cagggaaggc ctgttggacc ttttgaccag atgatagcgg gacatgctcg
    33421 cagtcgcggt ttgattgtgg ttaccaacaa tacccgagag tttgagcgag tggcgggaat
    33481 cagactagaa gactggagct aacattctct tttacttcta acattcgtaa attatataac
    33541 gtggcatgaa gccttaattc ctccgccatt tcatcccgct ttcctgtggt aatcttcctc
    33601 tgaaccacct acgatcattt ctataatgaa tatattgttg aggtatctat gcgttggcaa
    33661 gggcgacgtg aaagtgacaa tgttgaagac aggcgcaaca gctccggtgg tccgtctatg
    33721 ggcggtcccg gttttcgcct gccaagcggt aaaggcgggt tgattttact gttagtcgtg
    33781 ctggttgcag gctactatgg tgttgattta accgggttaa tgactggaca gccggtttcc
    33841 caacaacaat caacgcggtc cattagccct aatgaagacg aagctgcaaa attcacctcg
    33901 gtgattctgg caaccacgga agacacctgg ggacaacagt tcgagaagat gggtaagacc
    33961 tatcagcaac cgaagctggt catgtaccgt ggaatgacgc gcaccggctg cggggctggt
    34021 cagtccatta tggggccgtt ctattgcccg gcggatggca cagtttatat cgatctctcc
    34081 ttctatgatg acatgaaaga caaacttggc gcggatggcg attttgccca ggggtacgtt
    34141 atcgcccatg aagtcggtca tcatgtgcag aaactgttag gcatcgagcc gaaagttcgt
    34201 caactgcaac aaaacgcgac gcaggcggaa gtgaaccgct tatctgtgcg tatggaactc
    34261 caggccgact gttttgccgg tgtctggggg cgtatgcagc agcaaggcgt tctggaaacc
    34321 ggcgatctgg aagaggcgct gaacgcggcg caggccatcg gcgatgaccg tttacaacag
    34381 caaagtcagg ggcgagtagt accagatagt ttcacacatg gcacttctca gcaacgctac
    34441 agctggttta aacgtggttt cgacagcggc gatccggcac aatgcaatac ttttggtaaa
    34501 agcatttaac ttccggggca gggatggctg aactgactgc gcttcacaca ttaacagcgc
    34561 aaatgaaacg tgaagggatc cgccgcttgc tggtgttgag cggggaagag ggttggtgtt
    34621 ttgatcatgc gcttaagttg cgtgatgcct tacctggcga ctggctgtgg atttcgccgc
    34681 agccagatgc tgaaaaccac tgttcaccct cggcgctaca aactttactt gggcgcgagt
    34741 tccggcatgc ggtattcgac gcccgccacg gctttgatgc cgctgccttt gcagcactta
    34801 gcggaacgtt gaaagcggga agctggctgg ttttgttact ccctgtatgg gaagagtggg
    34861 aaaaccaacc tgatgccgac tcgctgcgct ggagtgattg ccctgaccct attgcgacgc
    34921 cgcattttgt ccagcatctc aaacgcgtac ttacggcgga taacgacgct atcctctggc
    34981 ggcaaaacca gccgttctcg ttggcgcatt ttactccccg tactgactgg caccccgcga
    35041 ccggcgcacc acaaccagaa caacagcaac tcttacagca gctactgacc atgcctccgg
    35101 gcgtggcagc ggtaacggct gcgcgtgggc gcggtaagtc ggcgttggca gggcaactca
    35161 tttctcgtat tgcgggcagt gcgattgtca ccgcgcccgc aaaagcggca acggatgtac
    35221 tggcacaatt tgcgggcgag aagtttcgct ttattgcacc ggatgccttg ttagccagcg
    35281 atgagcaagc cgactggctg gtggtcgacg aagccgcagc catacctgcg ccgttgttgc
    35341 atcaactggt atcgcgtttt cctcgaacgt tgttaaccac tacggtgcag ggctacgaag
    35401 gcaccggacg tggttttttg ctgaaatttt gcgctcgctt tccgcattta caccgttttg
    35461 aactgcaaca gccgatccgc tgggcacagg gatgcccgct ggaaaaaatg gttagtgagg
    35521 cactggtttt tgacgatgaa aacttcaccc atacaccaca aggcaatatc gtcatttccg
    35581 catttgaaca aacgttatgg cgaagcgagc cagaaacgcc gttaaaggtt tatcagttat
    35641 tgtctggtgc gcactaccgg acttcgccgc tggatttacg ccggatgatg gatgcaccag
    35701 ggcaacattt tttacaggcg gctggcgaaa acgagattgc cggagcgctg tggctggtgg
    35761 atgagggggg attatctcaa caactcagtc aggcggtatg ggcaggtttt cgtcgcccgc
    35821 ggggtaatct ggtggcccag tcgctggcgg cgcacggtag caatccactg gcagcgacat
    35881 tgcgtggacg gcgggtcagc cggatagcag ttcatccggc gcgtcagcgg gaaggcacag
    35941 ggcagcaact tattgctggt gctttgcaat atacgcgtga cctcgactat ctttcggtga
    36001 gttttggtta caccggggag ttatggcgtt tctggcaccg ctgcggtttt gtgctggtgc
    36061 ggatgggtaa tcatcgtgaa gccagcagcg gttgctatac ggcgatggcg ctgttaccga
    36121 tgagtaatgc gggtaaacaa ctggctgaac gtgagcatta ccgtttacgt cgcgatgcgc
    36181 aagctctcgc aaagtggaat ggcgaaacgc ttcctgttga tccactaaac gatgccgtcc
    36241 tttctgacga cgactggctt gaactggccg gttttgcttt cgctcatcgt ccgctattaa
    36301 catcgttagg ttgcttattg cgtctgctac aaaccagtga actggcatta ccggcgctgc
    36361 gtgggcgttt acagaaaaac gtcagcgacg cgcagttatg taccacactt aaactttcag
    36421 gccgcaagat gttactggtc cgtcagcggg aagaggccgc gcaggcgctg ttcgcactta
    36481 atgatgttcg cactgagcgt ctgcgcgatc gcataacgca atggcaattt tttcactgac
    36541 tccttcaatt ttctcccatg gtcattgtga gtcagcggcg taaaattaat gacatcagtt
    36601 aaggagttcg ccatgaaaca tgaccatttt gttgttcaaa gcccggataa acctgcgcaa
    36661 cagttgttac tgcttttcca cggtgtcggg gataacccgg tggcaatggg ggaaataggt
    36721 agctggtttg caccgctgtt tcctgatgcg ctggtggtga gtgtcggtgg cgcggagccg
    36781 agcggtaatc cggcggggcg tcagtggttt tcagtgcagg gtattacgga agataatcgc
    36841 caggcgcgtg tggatgccat catgccgacg tttattgaaa cggtacgcta ctggcagaaa
    36901 cagagcggag taggggctaa cgccacggcg ctaattggtt tctcacaagg cgcgattatg
    36961 gcgctggaga gcattaaagc cgaaccgggc cttgcttcgc gcgtcattgc ctttaacgga
    37021 cgctatgcga gcctgccgga gaccgcgtct acagccacga caattcactt gattcatggt
    37081 ggtgaagatc cggttatcga tctggcgcat gcagttgcgg cgcaggaagc gttaatcagt
    37141 gccggtggtg acgtgacgct ggatatcgtg gaggatttag gccatgcgat cgacaatcgc
    37201 agtatgcaat ttgccctgga tcacttgcgt tataccattc cgaaacatta tttcgacgaa
    37261 gcgctaagtg gcggtaagcc tggcgatgat gatgtgattg aaatgatgta aaagacagat
    37321 tacttggcct gataagacgc gtgagcgtcg catcaggcat cgttcaccat tgccggatgc
    37381 ggcgttaacg ccttatccgg cctacggttg agcactcagc ttacttcttc ggttgatcct
    37441 ttttcggcca gtcgtcgtca tcgtcccact tatcgttgaa atcacgatgc gggggaagct
    37501 ccggcttgtt ggcgagaaat ttcttatggt cgacgcgctt gagatcttta atcacgttca
    37561 gcaggacgcc taccaaaaag acaatcacca gaatccacca atatttagcc agccagtcca
    37621 tgcttatttc ctcttgcaga accactcatc aggcgacgag ctgttccatg atacgttgat
    37681 acatacgggc aagtagctgc aggttggcag cgttcacaca ttcattaatt ttatgaatag
    37741 tggcattgac cggcccgagt tccaccacct gcgcccccat gcgagcaata aagcgcccgt
    37801 cggacgttcc gcctgtggtc agtagctgcg gtttaatttc attatagtgc tcaaccgcgt
    37861 taacgaccgc atccaccagt ttaccgcgcg cggtcaaaaa tggctgcccg gaaagccacc
    37921 agtccaccgt ataacgcagt tgatgttttt caagcagggc aagcacctgt gctttgatca
    37981 tctcatcagt cagttcggtg ctgaagcgga agttaaactg cacaaacagt tcacccggaa
    38041 taacgttgtt actgcccgtt cccgcctgaa tattggcaat ctgcatactg gtcgccggga
    38101 agaattcatt gccctgatcc cactcaatag ccactaattc attaaggaaa ggtgctgcgc
    38161 gatgtaccgg attgtcagcc agatgcgggt aggcaacatg cccctgaacg ccatgaatgg
    38221 tgaggttgca ggttaacgat ccgcgacgac catttttcac cacatcacct accacttcga
    38281 tactcgacgg ttcgccgacc aggcagtaat cgagacgctc attacgtgcc attaacgctt
    38341 cgacgacttt taccgtaccg ttgtgtgcac tggcttcttc atcagaggtg atcagaaatg
    38401 ccagtcgccc cgtatggttg ggatgttgtg cgacaaagcg ttctgccgct accaccatcg
    38461 ccgccagcga gcctttcata tctgccgcac cgcgcccgaa taacatgcca tcacgaatgg
    38521 tgggttcaaa cggcggattg atccaacgat cggcgtcgcc aggcggcacc acgtcggtat
    38581 gcccggcaaa ggctaacgtt tcaccttgcc cacgccatgc ccaaaaattc tgcgtatcgg
    38641 caaagtccat gcgttcaacg gtaaaaccga tcgcctgcaa acgttcaatc aacaaagcct
    38701 ggcatcctgc gtcatcagga ctcagggaag ggcggcgaat aagctgttgt gtcagctcaa
    38761 taaccgggca cgacatagac tacacctcat ggaaaaattg ctgataactg gaatcactga
    38821 aacccagcag cataggctta ccaggcgcgc agagcaatgg acgtttgata attgcaggca
    38881 tttcagtcat taatgccgcc gcagaggccg catcggtgat tttattgcgg gtggtttcgt
    38941 ccagtttacg ccaggttgta ccacgggtgt tgagtaacgc ttcccagcct aattcgttga
    39001 taaaaccgtt caataattcg ctgtccagcc catcgacgcg gtaatcatga aaacgatagt
    39061 cgatgttatt ggcttccagc caacggcgag cctttttaat ggtgtcacaa tttttgatgc
    39121 cgtaaagtgt aaccataatg aatccttttt cgatttatga gaataaatac tcatttaagg
    39181 gg
//
LOCUS       DAZFWL020000031        39071 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFWL020000031 DAZFWL020000000
VERSION     DAZFWL020000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.031"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..1103
                     /gene="yeiH"
                     /locus_tag="ACHKNP_RS08670"
                     /old_locus_tag="pgaptmp_001734"
     CDS             54..1103
                     /gene="yeiH"
                     /locus_tag="ACHKNP_RS08670"
                     /old_locus_tag="pgaptmp_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGU4703921.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1177..2034
                     /gene="nfo"
                     /locus_tag="ACHKNP_RS08675"
                     /old_locus_tag="pgaptmp_001735"
     CDS             1177..2034
                     /gene="nfo"
                     /locus_tag="ACHKNP_RS08675"
                     /old_locus_tag="pgaptmp_001735"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HGU4703922.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2038..3126
                     /locus_tag="ACHKNP_RS08680"
                     /old_locus_tag="pgaptmp_001736"
     CDS             2038..3126
                     /locus_tag="ACHKNP_RS08680"
                     /old_locus_tag="pgaptmp_001736"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU4703923.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3182..4432)
                     /locus_tag="ACHKNP_RS08685"
                     /old_locus_tag="pgaptmp_001737"
     CDS             complement(3182..4432)
                     /locus_tag="ACHKNP_RS08685"
                     /old_locus_tag="pgaptmp_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU4703924.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4532..5473)
                     /gene="rihB"
                     /locus_tag="ACHKNP_RS08690"
                     /old_locus_tag="pgaptmp_001738"
     CDS             complement(4532..5473)
                     /gene="rihB"
                     /locus_tag="ACHKNP_RS08690"
                     /old_locus_tag="pgaptmp_001738"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HGU4703925.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5603..6301
                     /gene="yeiL"
                     /locus_tag="ACHKNP_RS08695"
                     /old_locus_tag="pgaptmp_001739"
     CDS             5603..6301
                     /gene="yeiL"
                     /locus_tag="ACHKNP_RS08695"
                     /old_locus_tag="pgaptmp_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HGU4703926.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6369..7619)
                     /gene="psuT"
                     /locus_tag="ACHKNP_RS08700"
                     /old_locus_tag="pgaptmp_001740"
     CDS             complement(6369..7619)
                     /gene="psuT"
                     /locus_tag="ACHKNP_RS08700"
                     /old_locus_tag="pgaptmp_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU4703927.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7713..8651)
                     /gene="psuG"
                     /locus_tag="ACHKNP_RS08705"
                     /old_locus_tag="pgaptmp_001741"
     CDS             complement(7713..8651)
                     /gene="psuG"
                     /locus_tag="ACHKNP_RS08705"
                     /old_locus_tag="pgaptmp_001741"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HGU4703928.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(8639..9580)
                     /gene="psuK"
                     /locus_tag="ACHKNP_RS08710"
                     /old_locus_tag="pgaptmp_001742"
     CDS             complement(8639..9580)
                     /gene="psuK"
                     /locus_tag="ACHKNP_RS08710"
                     /old_locus_tag="pgaptmp_001742"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HGU4703929.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRGDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(10004..11695)
                     /gene="fruA"
                     /locus_tag="ACHKNP_RS08715"
                     /old_locus_tag="pgaptmp_001743"
     CDS             complement(10004..11695)
                     /gene="fruA"
                     /locus_tag="ACHKNP_RS08715"
                     /old_locus_tag="pgaptmp_001743"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HGU4703930.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(11712..12650)
                     /gene="fruK"
                     /locus_tag="ACHKNP_RS08720"
                     /old_locus_tag="pgaptmp_001744"
     CDS             complement(11712..12650)
                     /gene="fruK"
                     /locus_tag="ACHKNP_RS08720"
                     /old_locus_tag="pgaptmp_001744"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGU4703931.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12650..13780)
                     /gene="fruB"
                     /locus_tag="ACHKNP_RS08725"
                     /old_locus_tag="pgaptmp_001745"
     CDS             complement(12650..13780)
                     /gene="fruB"
                     /locus_tag="ACHKNP_RS08725"
                     /old_locus_tag="pgaptmp_001745"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HGU4703932.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQLAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFTEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIA
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            14148..15329
                     /gene="setB"
                     /locus_tag="ACHKNP_RS08730"
                     /old_locus_tag="pgaptmp_001746"
     CDS             14148..15329
                     /gene="setB"
                     /locus_tag="ACHKNP_RS08730"
                     /old_locus_tag="pgaptmp_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HGU4703933.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(15326..15580)
                     /gene="yeiW"
                     /locus_tag="ACHKNP_RS08735"
                     /old_locus_tag="pgaptmp_001747"
     CDS             complement(15326..15580)
                     /gene="yeiW"
                     /locus_tag="ACHKNP_RS08735"
                     /old_locus_tag="pgaptmp_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU4703934.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15735..16307
                     /gene="yeiP"
                     /locus_tag="ACHKNP_RS08740"
                     /old_locus_tag="pgaptmp_001748"
     CDS             15735..16307
                     /gene="yeiP"
                     /locus_tag="ACHKNP_RS08740"
                     /old_locus_tag="pgaptmp_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HGU4703935.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16530..17996
                     /gene="yeiQ"
                     /locus_tag="ACHKNP_RS08745"
                     /old_locus_tag="pgaptmp_001749"
     CDS             16530..17996
                     /gene="yeiQ"
                     /locus_tag="ACHKNP_RS08745"
                     /old_locus_tag="pgaptmp_001749"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU4703936.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLVEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHQGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            18114..19100
                     /gene="yeiR"
                     /locus_tag="ACHKNP_RS08750"
                     /old_locus_tag="pgaptmp_001750"
     CDS             18114..19100
                     /gene="yeiR"
                     /locus_tag="ACHKNP_RS08750"
                     /old_locus_tag="pgaptmp_001750"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HGU4703937.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            19139..19852
                     /gene="lpxT"
                     /locus_tag="ACHKNP_RS08755"
                     /old_locus_tag="pgaptmp_001751"
     CDS             19139..19852
                     /gene="lpxT"
                     /locus_tag="ACHKNP_RS08755"
                     /old_locus_tag="pgaptmp_001751"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HGU4703938.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            20264..20830
                     /gene="mepS"
                     /locus_tag="ACHKNP_RS08760"
                     /old_locus_tag="pgaptmp_001752"
     CDS             20264..20830
                     /gene="mepS"
                     /locus_tag="ACHKNP_RS08760"
                     /old_locus_tag="pgaptmp_001752"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HGU4703939.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            21011..22567
                     /gene="pdeN"
                     /locus_tag="ACHKNP_RS08765"
                     /old_locus_tag="pgaptmp_001753"
     CDS             21011..22567
                     /gene="pdeN"
                     /locus_tag="ACHKNP_RS08765"
                     /old_locus_tag="pgaptmp_001753"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGU4703940.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            22649..24463
                     /gene="yejA"
                     /locus_tag="ACHKNP_RS08770"
                     /old_locus_tag="pgaptmp_001754"
     CDS             22649..24463
                     /gene="yejA"
                     /locus_tag="ACHKNP_RS08770"
                     /old_locus_tag="pgaptmp_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGU4703941.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDTFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            24464..25558
                     /gene="yejB"
                     /locus_tag="ACHKNP_RS08775"
                     /old_locus_tag="pgaptmp_001755"
     CDS             24464..25558
                     /gene="yejB"
                     /locus_tag="ACHKNP_RS08775"
                     /old_locus_tag="pgaptmp_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HGU4703942.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSKYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            25558..26583
                     /gene="yejE"
                     /locus_tag="ACHKNP_RS08780"
                     /old_locus_tag="pgaptmp_001756"
     CDS             25558..26583
                     /gene="yejE"
                     /locus_tag="ACHKNP_RS08780"
                     /old_locus_tag="pgaptmp_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4703943.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            26585..28174
                     /gene="yejF"
                     /locus_tag="ACHKNP_RS08785"
                     /old_locus_tag="pgaptmp_001757"
     CDS             26585..28174
                     /gene="yejF"
                     /locus_tag="ACHKNP_RS08785"
                     /old_locus_tag="pgaptmp_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HGU4703944.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(28178..28522)
                     /gene="yejG"
                     /locus_tag="ACHKNP_RS08790"
                     /old_locus_tag="pgaptmp_001758"
     CDS             complement(28178..28522)
                     /gene="yejG"
                     /locus_tag="ACHKNP_RS08790"
                     /old_locus_tag="pgaptmp_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HGU4703945.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(28855..30045)
                     /gene="bcr"
                     /locus_tag="ACHKNP_RS08795"
                     /old_locus_tag="pgaptmp_001759"
     CDS             complement(28855..30045)
                     /gene="bcr"
                     /locus_tag="ACHKNP_RS08795"
                     /old_locus_tag="pgaptmp_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HGU4703946.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            complement(30073..30768)
                     /gene="rsuA"
                     /locus_tag="ACHKNP_RS08800"
                     /old_locus_tag="pgaptmp_001760"
     CDS             complement(30073..30768)
                     /gene="rsuA"
                     /locus_tag="ACHKNP_RS08800"
                     /old_locus_tag="pgaptmp_001760"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HGU4703947.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            30917..32677
                     /gene="radD"
                     /locus_tag="ACHKNP_RS08805"
                     /old_locus_tag="pgaptmp_001761"
     CDS             30917..32677
                     /gene="radD"
                     /locus_tag="ACHKNP_RS08805"
                     /old_locus_tag="pgaptmp_001761"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU4703948.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGNEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            32802..33086
                     /gene="rplY"
                     /locus_tag="ACHKNP_RS08810"
                     /old_locus_tag="pgaptmp_001762"
     CDS             32802..33086
                     /gene="rplY"
                     /locus_tag="ACHKNP_RS08810"
                     /old_locus_tag="pgaptmp_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HGU4703949.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(33225..34232)
                     /gene="yejK"
                     /locus_tag="ACHKNP_RS08815"
                     /old_locus_tag="pgaptmp_001763"
     CDS             complement(33225..34232)
                     /gene="yejK"
                     /locus_tag="ACHKNP_RS08815"
                     /old_locus_tag="pgaptmp_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HGU4703950.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            34414..34641
                     /gene="yejL"
                     /locus_tag="ACHKNP_RS08820"
                     /old_locus_tag="pgaptmp_001764"
     CDS             34414..34641
                     /gene="yejL"
                     /locus_tag="ACHKNP_RS08820"
                     /old_locus_tag="pgaptmp_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HGU4703951.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            34661..36421
                     /gene="yejM"
                     /locus_tag="ACHKNP_RS08825"
                     /old_locus_tag="pgaptmp_001765"
     CDS             34661..36421
                     /gene="yejM"
                     /locus_tag="ACHKNP_RS08825"
                     /old_locus_tag="pgaptmp_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HGU4703952.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            36496..36572
                     /locus_tag="ACHKNP_RS08830"
                     /old_locus_tag="pgaptmp_001766"
     tRNA            36496..36572
                     /locus_tag="ACHKNP_RS08830"
                     /old_locus_tag="pgaptmp_001766"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36530..36532,aa:Pro,seq:ggg)
     gene            36791..38032
                     /locus_tag="ACHKNP_RS08835"
                     /old_locus_tag="pgaptmp_001767"
     CDS             36791..38032
                     /locus_tag="ACHKNP_RS08835"
                     /old_locus_tag="pgaptmp_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HGU4703953.1"
                     /translation="MARKTHPLTTVQIKAARPAQKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLEDVSLADARKRRSEYRTLISAGTDPQDHERQKRETEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            38131..38361
                     /locus_tag="ACHKNP_RS08840"
                     /old_locus_tag="pgaptmp_001768"
     CDS             38131..38361
                     /locus_tag="ACHKNP_RS08840"
                     /old_locus_tag="pgaptmp_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703954.1"
                     /translation="MQKNIRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNLAVSIDKVVRPASMKIGGRPKKWR"
     gene            38529..38735
                     /locus_tag="ACHKNP_RS08845"
                     /old_locus_tag="pgaptmp_001769"
     CDS             38529..38735
                     /locus_tag="ACHKNP_RS08845"
                     /old_locus_tag="pgaptmp_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4703955.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLSWQKEREEEARGTAA"
     gene            38750..>39071
                     /locus_tag="ACHKNP_RS08850"
                     /old_locus_tag="pgaptmp_001770"
     CDS             38750..>39071
                     /locus_tag="ACHKNP_RS08850"
                     /old_locus_tag="pgaptmp_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4703956.1"
                     /translation="MTNTKKAAPIWSRLSEQLTRCALCVCDPKHKHGDDSRYQAGGQC
                     NQSGSVRCHTCNERFSLYSLRNCSRAKAHGANLSDSCSIFLRRLFRAGDNVLVNSLSV
                     IVLSC"
BASE COUNT         9674 a        10123 c         9627 g         9647 t
ORIGIN      
        1 tttattttta aaccagttag tcgtaacctt atgcccggtt aaaggagagg gttatgacga
       61 atatcacctt acagaaacaa catcgtacac tgtggcattt tattccgggg ttagccctga
      121 gtgcagttat caccggggtc gccctgtggg gtggttccat tcccgcggtt gcgggtgccg
      181 ggtttagtgc cctcaccctc gcaatcttgt tggggatggt tttaggcaac accatctatc
      241 cgcacatctg gaaaagctgt gacggtggtg tgctgtttgc caagcaatat ttattacgtc
      301 tgggtatcat tctttatggc ttccgtctga cgttctcgca aattgccgat gtcggtatca
      361 gtgggatcat cattgacgtg ttgacgctgt ccagtacctt cctgctggct tgcttcctgg
      421 ggcagaaagt gtttggtctg gataagcaca ccagctggtt gatcggtgcc ggtagcagta
      481 tctgtggtgc tgccgcggta ctggcgactg agccggtagt gaaagcggaa gccagtaaag
      541 taaccgtggc tgttgcaacc gttgttatct tcgggaccgt cgctattttc ctctacccgg
      601 cgatatatcc gctgatgtct caatggttta gtccggaaac tttcggtatc tacattggtt
      661 ctactgtgca cgaagtggcg caggtggtgg cggcaggtca tgccatcagc ccggatgcgg
      721 aaaacgcagc agttatttcc aaaatgctgc gcgtgatgat gctggctccg ttcctcatcc
      781 tgctggcggc gcgtgttaaa cagctgtctg gggcgaacag cggcgagaaa agcaaaatca
      841 ctattccgtg gtttgccatc ttgttcatcg tagttgccat ctttaactcg ttccacctgt
      901 taccgcagag cgtggtgaac atgctggtaa cgctggatac cttcctgctg gcaatggcga
      961 tggcggcgct gggtctgacc actcacgtca gcgcgctgaa aaaagccggg gcgaaacctc
     1021 tgctgatggc actggtactg tttgcctggc tgattgttgg tggtggtgcg attaactatg
     1081 tgattcaaag cgtcatcgca taaaccacta catcttgctc ctgttaaccc gctatcatta
     1141 ccgttttcct ccagcgggtt taacaggagt cctcgcatga aatacattgg agcgcacgtt
     1201 agtgctgctg gcggtctggc aaatgccgca attcgcgccg ccgaaatcga cgcaaccgcg
     1261 tttgccttgt tcaccaaaaa ccaacgtcag tggcgtgccg caccgctcac gacgcaaacc
     1321 atcgatgaat tcaaagcggc ctgtgaaaaa tatcactaca catcggcgca aattcttccc
     1381 cacgacagct atctgattaa cctcggacat ccggtcactg aagctctgga aaaatcgcgc
     1441 gatgccttta tagatgaaat gcagcgttgc gaacaactgg ggctttcttt gctcaacttc
     1501 caccctggca gccatctgat gcagatttca gaagaggatt gccttgcgcg tattgccgaa
     1561 tccatcaaca ttgcgctgga taaaactcaa ggtgtaacag cggtgattga aaacaccgcc
     1621 ggtcagggca gtaacttagg gtttaaattc gaacatctcg cggcgattat cgacggcgtg
     1681 gaagataaat cccgtgtcgg cgtctgcatt gatacctgcc atgctttcgc tgccgggtat
     1741 gatttgcgta ctccagccga atgcgaaaaa acgttcgctg attttgcccg tattgttggc
     1801 tttaagtatc tgcgcgggat gcaccttaac gatgcgaaaa gcacctttgg cagccgcgtt
     1861 gaccgccatc atagcctcgg tgaaggcaat atcggtcatg atgcgttccg ctggatcatg
     1921 caggacgacc gtttcgacgg cattccgctg atccttgaaa ccatcaaccc ggatatctgg
     1981 gcagaagaga tcgcctggct gaaagcgcaa caaactgaaa aagcggtggc ctgatcgatg
     2041 aacgacaggg aaaagcagat tcttaaaatt ttacggcgta acccgctgat tcaacagaat
     2101 gaaatcgccg atattctgca aatcagccgc tcacgtgttg ccgctcatat tatggatttg
     2161 atgcgcaagg gtttgattaa aggcaaagga tatatcctta ccgagcagga ttactgtgtg
     2221 gttgtgggtg caatcaatat ggatattcgc ggaatggcgg atatccgtta cccgcaagca
     2281 gcctctcatc caggttctgt tcactgttcc gcaggcggcg tcggacgtaa tatcgcgcat
     2341 aacctggctc tgcttggtcg ggatgtgcat ttaatatcag ctattggcaa cgatttttac
     2401 ggcgagacat tactggaaga aacgcgccgc gcgggcgtga acgtttcaaa ctgcattcgt
     2461 ctgcatggac acagcactgc aacttatctc gctatcgcta ataagcagga agaaactatc
     2521 ctggcaatca atgacacaca tattctgcaa cagctaacgc ctcaactgct caatacttca
     2581 cgggacttga ttcgccatgc tggtgtggtg ctggctgatt gtaatctcac acctgaagcc
     2641 ctggagtggg tctttaccat tgctgatgaa attccgatgt ttgtcgatac cgtttccgaa
     2701 tttaaagcga acaaagtaaa aaactggtac agccggatcc acactctgaa accaacacaa
     2761 aatgaactgg aaattttgtg ggggcaaccg attaaagacg acaacgatcg catcagagct
     2821 gtaaattcgc tgcatcaaca aggtgttaaa cgcatttttg tttatctgaa agatgaatct
     2881 gtattttgca gcgataagga tggagaacaa tttttgctga ccgcgccagc gcatacgaca
     2941 gtagacagtt ttggtgctga cgatggtttt atggcgggcc tggtatatag ctttcttgaa
     3001 ggatacagtt tccgcgacag cgcccgtttt gcggtagcct gcgcggcaat ttcgcgcgcc
     3061 agcggcagct taaacaaccc taccctgtct gccgataacg cgctttcatt agtgccaatg
     3121 gtgtaacaat gttgccggat tcgacgcttg accgcatctt atccggctca taaattgcgc
     3181 actacgctaa accaataaag aatcctgcaa tagtagcact catcaggtta gaaagtgtcg
     3241 ccgccgccag cgcgcgtaaa ccaagttggg cgatttccgg cgcacggtgt ggcgcaaccg
     3301 cagaaaacgc ccccaccacc accccgatag aaccaaagtt agcgaaaccg cacaacgcga
     3361 aagaaataat cgccacggtt ttagcatcca gagtgccacc cgtttgcaga tatggcgaga
     3421 aattgagata agcgacaaat tcattgatcg ccagcttctg cccaatcaaa ctcccggcaa
     3481 gatttgcatc actccagtca acccccatca cccacgccaa tggggccaac aggtaaccta
     3541 aaatggactc cagcgaggca tgtgcaaaac caaaccagcc gccaacgccg ccgataatac
     3601 cattaattaa cgcaatgatg gcgacaaatg ccataacaac tgtcgctaca ccggcggcga
     3661 ttttcagccc ggtcattgcc cctgtcgcag cggcttcaat aatgcttttt ggcggtgttt
     3721 cggtgaaaga gaggttatta aaggaaacct gcgaagattc cgtagccggg cttaacaggc
     3781 gggcaaacaa gatcccgcct gggatcgcca ttaacgatgc cgccagcaaa tattccacag
     3841 gtacgcccag ggcggcgtaa ccaatcattg tcgaaccagc aatcgaggcc atgccactac
     3901 aaatcgctgt aaataattca ttgcgattca gacgatcgat aaagggtttg acgattgccg
     3961 gaatttcgtt ttgcccgagg aaaatggtag tgaccgcgac gaatgactcg atcttgctga
     4021 tatttaacgc tttctggaag ataccgccga gaatgcgaat taaaatcccc atcacaccga
     4081 tgtagtagag aatactcacc agtgctgtaa cgaagataat tgccggtaat actctgaaac
     4141 caaagataaa tcctgcgcca tcaaacagcg tgtccatttt cggcccgacc agcgaaccga
     4201 agataaatgc gctacccgcg tcgctgtacg ccatcacttt atgcacgcca aaagcgactt
     4261 tttcagcgac ccaacgccct ggcggtaacc aaagcataat gccgccaatc acaacctgta
     4321 acactaacgc cgcgccaacg gtacgcaggc tgatcttctt cttgtttact gacagcaaaa
     4381 atgcaattgc cagcaatacc accattccca gaacacttct catgacatcc atagtgatta
     4441 tctcttcatg tcatattggg cgacggcttt ctgcccatca gaaatagccg ggcctgcatc
     4501 ttgcgctatc ggcccggctt gttatcaacg cttaatgggt tttgatgtag ccgcgcacgc
     4561 actcttcgac taagccccag aaccagtcgg tatcaatagt gatgccgact ttggtattgg
     4621 cgggcttgcc aagaacgccc agctcgtcgc agacggtgcg cccatagcaa gggccactgt
     4681 tgacgtccac ttcgacgtac atctcctggg ttttaatgcc atcagggtta atcagataac
     4741 cgatgcaggt ggcgtcgtgc accgggccgc cagccaggcc gtagttttcg aactgcgttt
     4801 tgagagtgaa gttcatgatg tcgctgaaca gctctccggc agggccgcct gccctttcca
     4861 tccgagcaat cacgtccggg gtgcaaacgg tctggttggt gagatcgagg cccatcatca
     4921 ctaatggaac gccagaggtg aacaccacgc gggctgcttc cgggtcagca aagatgttga
     4981 actcggcaga tggcgtgaag ttgcctgtac cgtaagcgcc gcccattagc acaatttcac
     5041 ggattttggg caggatcgcg ggttgcatac gcattgccac cgcgatattt gaaagcggac
     5101 caaccggcac cagagtgata tcgccatcgc ttgccatcag ggtatcaatg atgtatttca
     5161 ccgcgtgggt actttctgcc tggcgggtta gcggctcgaa taccgggcca tccagtccgg
     5221 tttcaccgtg aatattatcg gcaacgattt gttgacgcat aatgggttgc ggcatccccg
     5281 cgtaaaccgg aacattaatc tccagtttct ggcaaacatt caggccatta attaatgttt
     5341 tatcaagcgt ctgattaccc gctacaatag tgatgcctaa taaatctatt gccggatgtt
     5401 tcgctgccat catcattgca atagcatcat catgacccgg atcacaatcc agaataattt
     5461 ttctcttttc cattgtttat ttcctctgtt tccagttgcg ttattttttc tacagcaaag
     5521 aaaattcgac cgggccgatg attgaatctt accaaccgcg tacgtatgct aaatatgaga
     5581 aatctcatag cggataaaca tcgtgaaaga aatccacaat aatgatctta agcagcaatt
     5641 gatgagtgaa tccgcgttta aggattgctt tttaacggat gtttcagccg atacgcggct
     5701 gtttcatttt ttagcgcgtg actacattgt gcaggaaggg caacagccgt cctggctgtt
     5761 ttacctgacg cgaggccgcg ccaggcttta cgccacgcta gctaatggtc gcgtgtcgct
     5821 gatcgatttc tttgccgccc cctgttttat tggcgagatt gagttaatcg acaaagacca
     5881 tgaaccgcgt gcggtgcagg ctattgaaga gtgttggtgc cttgcgctcc ctatgaaaca
     5941 ttaccgcccg cggttattaa acgacacgct atttttacga aaactctgcg tcaccttaag
     6001 tcataaaaat tatcgtaata ttgtttcttt aactcagaat caatcatttc cgttagtgaa
     6061 tcgcctggca gcatttatat tactctcgca ggaaggtgat ctttatcacg aaaagcatac
     6121 gcaagcggca gagtatttag gcgtttctta tcgacatctt ttatatgttc tcgcgcaatt
     6181 cattcacgac ggtttattaa ttaaaagcaa gaaagggtat ctaattaaaa acagaaagca
     6241 gttgtcagga ctggcgctgg agatggaccc ggagaataaa ttctccggga tgatgcagta
     6301 aaaattattt gcaatagcgc gattgccgga tgcaacactt aataagcgcg tacatccggc
     6361 aatgaaactc aagctaaacc aataaagaac ccggcaatgg tcgcactcat caggttggaa
     6421 agcgttgctg ctgccagcgc ccgtaaacca agctgggcga tttccggcgc gcgttttggc
     6481 gaaatagccg aaaatgcgcc aacgacaaca ccgatagaac caaagttagc aaaaccacaa
     6541 agcgcaaagg agataatcgc aatggttttc acttccagcg tgccgcccgt ttgcaggtat
     6601 ggggagaaac tcaggtaagc gacgaattcg ttaatcgcca gtttctgccc aatcaggcta
     6661 cccgcaagat tggcatcact ccagtccaca cccatgatcc acgccagcgg tgccagcaca
     6721 tagccaaaaa tactttccag agaggcattg gcgaaaccaa accagccacc aattccgccg
     6781 ataatgccgt tgatcagcgc aataattgcg acaaacgcca ttaccaccgt cgctacacca
     6841 gcggcgattt tcagcccggt catcgcaccg ctcgccgccg cttcgataaa gctttttggc
     6901 ggcgtttcgc tgaacgacag attttcaaat gtgacctgtg aaggctcggt ggccgggcta
     6961 agaatacgtg caaacaaaat accacctggg atcgccatca gcgatgccgc taacaggtag
     7021 tcaattggta cgcccattcc ggcataacca atcatcatcg aaccagcaat ggacgccatc
     7081 ccgctacaaa ttgcggtaaa caactcgttg cgattcatgc gatcgataaa cggtttaacg
     7141 atcgccggga tctcattttg cccgaggaaa atagttgtga ctgcgacaaa agattcgatt
     7201 ttgctgatgt taagagcttt ctggaaaatg ctgccaagga tgcgaatcag cagccccatc
     7261 acgccaatgt agtacagcag actgatgagc gcagtaacga aaataatcgc cggaagtacg
     7321 cgaaaggcga agataaaacc cgcaccgtca aacaggacat ccattttcgg cccaaccagc
     7381 gaaccaaaaa tgaaggcgct accggcatca ctgtaagaca tcactttatg aacgcctaat
     7441 gccgcctgtt ctactgccca ctttcccggt gggaagtaga gcatgatgcc accaatagcg
     7501 atttgcagca gcagtgcggc tccaaccgtg cgcaaactga tgctcttttt attcactgac
     7561 aacagaaatg ctattgccag taacaccacc atccccacaa cacttctcat tatatccata
     7621 atgattttcc cttcatgcca gtaaacccgg cgtcagcgcc aggttttggt atgcttgatg
     7681 agtacgggcg acggctttct gcccgtcaga aattaacccg cgagacgctg gtattctttg
     7741 gcaatttcac tcgccagaat ggcgttgttg aacaccagct ggatattgga tttcaggctg
     7801 tcaccgccgg tcagctcagc aacgcgcgcc agcaggaacg gtgtactttc tttaccaata
     7861 acgccctgct cttcggcttc ggcgacggct tgatctatgg ctgcattgat agattcctcc
     7921 ggcatcgcaa actgttccgg gatcggattc gccactacca ggccgccgtt caaaccggtt
     7981 tgccatttca ctgccattgc acgagcgatt tccgtggcac tatcaaggcg aatactgact
     8041 tcaaatgggc tggtacggca gaaaaacgca ggcagcgctt ttgtctgata gccaattaac
     8101 ggcacaccga aggtttctaa atattctgtt gttaatccta aatcgagaat agatttcgcc
     8161 ccggcacaaa caacggtgac attagtattt gccagttctt gcaaatcggc agaaatatcg
     8221 aaggtatgtt ccgccccgcg atgcacacca ccaattcccc cggtggcaaa tactttaatt
     8281 ccggcaagcg ccgcaataat catcgttgaa gccacagtgg ttgcgccatt ttttccggcg
     8341 gcaacaacaa aaggtaaatc gcgacgacta actttggtca cgttatgccc ttcacgaccc
     8401 agtaattcaa tttcttcttt gcttaaaccc actttcatca cgccgccaat aatggcgata
     8461 gtggcaggta ctgcgccctg tttacgaata gtttcttcta cttcaattgc ggtctgggca
     8521 ttttgtggga acggcatccc gtgagaaata atggtcgatt ccagcgccac aaccggtttt
     8581 ttgtttttta aagcgtcctg cacttccggg gaaatttgta ataattcagg ggaaattttt
     8641 aattcagaca ttctgcgttc tccactaacg atataacgtt ggcaatcgat aaatcggggt
     8701 tattggtgta ttcacaggag agcgccattg acgaacatcc ctgtgcgaaa cgaacagatt
     8761 cggcaaacgg cattccgtct acccaacacg aagcaagtcc cgccatcatg gcatcgcccg
     8821 ctccggtaac attaataaca ttggttttga tcggggcaga ccagccactt tcaccgctga
     8881 tatcactgta ataaacgcca tcgccgccca tgctcaatac cagtcggttc aggccatgtt
     8941 gatggaacca ggcagcaact tttgccacat ctccacgccc tgacagcgca atcccactca
     9001 gtgtttccgc ttcaaggcgg tttggcttca gagtgtggat ctgatttaga cggtcgcgca
     9061 ctttgacaca tttccacgct gaaaccggat cgacaaatac gggtacgttg gcagcattat
     9121 ccagaatcca tgccagtgcc tcttcactga tattacagtc cgcgacaatg acctttgccc
     9181 tctgaataaa ttcacggtgc tgtgcgagat attcagctgt aatagcgttg ctaatattca
     9241 tgtcatttat agcaaccagc atttcaccgg tattatcgag taatgataaa taactcgacg
     9301 tattttctcc cggcacaatc aggcatttat cgacataaac gccagattga ttggtttgcg
     9361 ttagcagcga ttgaccataa aaatcactac ctactgcgct cagtaaccag gctttgttac
     9421 ccagcaacgc caggttttgt gcaatatttc gccctactcc accaggtgta aattttattt
     9481 tacctggatt tgaatccgca taatttaatg attcatgtga atatccggcg acatcaatat
     9541 tcgccgaacc tataattacg acataatcct tttcgcgcat agcacgtcct ctggtaaaaa
     9601 attaccaacc ctgtcaatcc acaagaatat agatactgca aagacagttt aaacatgtgt
     9661 tcatatttaa acatgtgctc atagtatgta tgttttacgt aaagtaaatt gccgccagag
     9721 aggcaaagcg ggcagatttg agcaggctca caaaatgcga ttaaatgcca gaattacaaa
     9781 agcgaggaat ttgtgacggg atgcacagaa caggaacact aaaaaagggc agaaaagaat
     9841 ctgcccttga ggaaataacc ttagtgtttg atgaacgttt caggtttcta ctgaaagata
     9901 aaaccattta aattcataaa attacattca caaaccggtc attctctttg cgctcatcca
     9961 atgatacagg gcagtaaatt tctgccctgt aacacacctt ttattacgcg gctttcgcta
    10021 ctgcgtccac ttccggacgt ttcaggaagg cataggccaa acccgccacc agcgtaccgg
    10081 caataattgc taccaggtaa cccagtaccg gcgtaatagc gccagggatc agcagaacaa
    10141 acagaccacc gtgcggtgcc atcagtttcg caccaatcgc cattgagatt gcgccagtca
    10201 gcgccccacc aacgatacaa cacggcagca cacgcatcgg atcacgagca gcaaacggaa
    10261 ttgcaccttc cgaaatgaag cacagtccca ataccagagc ggctttgcca ccttcctgct
    10321 gcgctttgtc gaatttgcga cgcgccacca ttgttgccag acccattgcc agcggtggca
    10381 ccatacctgc cgccataatc gccgccatcg ggccataggt ttgagtactc agcagaccca
    10441 caccgaatgc gtacgctgct ttgtttaccg gaccgcccat gtcagtacac atcatgccac
    10501 cgaggatcgc ccccagcaga accgcattcg cagtccccat ggtctgcagc cagtgagtca
    10561 acccttcgag aatgccagca actggtttac cgatcaggta gatcatcgcc agaccgacca
    10621 ccagactgga aattagcgga atgatcagga tcggtttcag cgcctccata ctctgtggca
    10681 gtttcagttg cgtactgatt aacttcgcaa tgtaaccagc caggaagccc gcaataatac
    10741 cgccaatgaa gccagaaccg gtgctgaccg ccagcatacc gccaatcaga cccggcgtga
    10801 gacccggacg atcggcaatg gagaaggcaa tgtaacctgc cagtaccggc accatcagcg
    10861 caaaggctga accaccacca atctgcatca gcgccgctgc caacgtaccc ggctctttaa
    10921 acgcttcgat accaaaagca aaagaaagcg cgatacacag accacctgca acgaccatcg
    10981 gcagcatgta agagacgccc gtcagcaagt gacggtatgc gcctgcactc tctttcttac
    11041 cttcagaggt cgccgtttga gctttgcccg ccggttcata cggcgttgct tcagcaaccg
    11101 ctttatccag ttcctgcgcg gttttcttca gcgccagacc ggtagaggta cgatacatcg
    11161 gtttaccagc aaatttcgcc agatccactt cgatatctgc cgccacaatc accagatccg
    11221 ctgcggctac ttcttcgggg gtgattgcat tacccgcacc aacagaacca cgggtttcaa
    11281 ctttcaccca ccagccacgt tttttcgctt cggtttcaat ggcttcagcc gccataaagg
    11341 tgtgtgctac gccagtcggg caagcagtca ccgcaactac gcgtttcgga ccgctggcgg
    11401 caaccggtgc tgtcgcagcg accggcgcag tgtaaggttt cgcatggcct ttggcttcac
    11461 tcaggaacag ctcagggtgc gcaactgccc gggaaatatc acccagccag acatttttac
    11521 cgttcagcgc gctgtcattc gggatggaat caccgagaac aatcgccatt tctgcgtcgt
    11581 tcggattgtc gatgatttcc agttttgctt ttcgcgccgc cgcgcccagc agggtcttcg
    11641 ccatataggc gcgtgcctga ccgagattag cgtcaataat cagcagcgtt ttcattatgc
    11701 ctctcctgct gtcagttaaa aggttgtaag tcgacgcgcg ccatcattgc ggccaactgc
    11761 ggacgatcgg taatacccac attgctttga cttaccgcca gggctgcaac agcagtcgcc
    11821 agacgcagtg tgtgttcact ggattcacgc atcagcaagc cataaatcag gccaccaacc
    11881 atagaatccc ctgcgccaac ggtgcttacg acatcgactg acggtggttt ggcgatccat
    11941 tcgccggagg cattaaccca aagcgcgcct tcggcaccca gtgaaataac aacatgcgca
    12001 ataccttgtt cacgtagcgc atgtgcagct tcaatcacat ctttcatttc aggcagttta
    12061 cggcctgccc agatttccaa ctcgcggcgg ttaggtttca ccagccacgg tgccgctttc
    12121 aaacctgcta ctaacgcttc acggctacta tcaaagataa tgcaaggaca ctgactacgc
    12181 aggcgagtca tccagtcggt gaacgcttcc gggctgacgc ctgacggtaa gcttccgctg
    12241 acacagacca tatcgaactg accgagccag ctcagagaat cagtcacaaa gcgttcccag
    12301 tcggcggggg tgacttcaaa acccgagaag ttgaagtcgg tcacttcgcc gtctttttcc
    12361 gtcagcttaa cgttaattcg ggtgcgcccc tgtacaacct ggaaacggtt ggcaatgccc
    12421 agctcgctga acagttgctg aaaaccatcc tgattgtctt tacccaggaa gccgccaacg
    12481 gtgacatcaa ttcccaggtc ttttaatact ttggccacgt tgatgccttt acccgccgca
    12541 tgcagaccgg tggttttcac caggttcact tcgccgcgtt caatttccgg gcagaaacca
    12601 acaaggtcat aagccggatt aagggtgata gtagcaacac gtctgctcat tatgcgccct
    12661 ccccaagacc agcagcgata gcgtcgccga ttgctttcag cgcctgttca gcatctgcac
    12721 cctgggcggt aaagcgtagg cgatgacctt tcttaacgcc aagtgccaca actttcatca
    12781 gactacgtcc gtttgccggt ttgccggtgc catcaaggtt tgtcacggta atatcactgt
    12841 taaattgttt aatggtgttg accagcatgg tacctggacg agcatgcagg ccgtgttcat
    12901 tgcgcaccac aaactccgcg cttaacacgt cgtcggtcgg cgcatcatcg ctggtcagca
    12961 gcgccagcaa cgttgccgca tccgctttca gcaagcggtc agctttattg tcgagcaaca
    13021 aatcagcgag acgcttaaga accgcaatgg gctgatcgtc attcatcgcc acactcacca
    13081 gcatggctgc cgtttcgccg tccacatcaa aagcatttgc cgcacggctt accgcaatcg
    13141 cgctacgcag attgccttcg gcgctatcgc tcagccagat accctgtccg aggttcagcg
    13201 gttgttcatt gatggctttg gtgacgaaag tggcgtcaac tgccctcgcc tctttcagac
    13261 gcgcagcgtt cagcgcctga agagtcagca gatcgctggc ggcgatatcc agcgtcagca
    13321 tttcgttgtc gagcttcagc tgctcactct gcttttcgcc catcagcaat gcgcgaagtt
    13381 cttctgctgt tgttgctgac ttcagttgtt cagcaacgga atcatcgctc agtacgtggg
    13441 tcagctggcg tagcaggccc agatgttcat ccgagctggc agcaataccg atcgctacgt
    13501 acgctacctg accgtcaccc caggtgacgc cttccgtgaa ctgaaatacc tgaacgccgg
    13561 ttttcagcac ctgatcgcgg gtgtcggtag tgccgtgtgg aatagcaata ccattgccga
    13621 ggaacgttga ggtttgctgt tcgcgcgcca gcatgccatt gacgtagcct tctgctacgt
    13681 taccggcctg caccagcgcc gcagcgagct ggcgaatcgc ctcttctttg tctccggcct
    13741 tttcgcccgg atggatgtcc tgtacggata actggaacat agttctcctc tcttgctgaa
    13801 ttgaaacgat tcagcttcta tgagaaaaaa agcgccaacc tggcttaggg ttaaagacaa
    13861 gatcgcgctg aaacgtttca agaaagcata atacttctgt ttcagcacgc aaggaaagcc
    13921 gtaaagttat gagcaaaagt ttgatgtgct gcacaatttt ttgtcatttt tcctaattgc
    13981 tgatgggaaa atccgttatc agcaatttca tttcagcagc gtcagcagcc aatcagcaac
    14041 agtgaaatgg cattttgatt attcgtggcg cttttgattt cgctgtctgt ttattttctg
    14101 acaagcagcg taaactccgc gtcttcctct tccagtgatc gaccagcatg cataactccc
    14161 ccgcagtctc cagcgcgaaa tcgtttgacc tgacctcgac ggcgttttta atcgttgcct
    14221 ttctcaccgg tattgcgggc gctctgcaaa ccccgacact cagtattttt cttaccgatg
    14281 aagtacatgc ccgtccggcg atggtgggat tcttctttac cggcagcgct gtcattggga
    14341 ttctggtaag tcagtttctc gccgggcgct ctgataagcg cggcgatcgc aaatcgctga
    14401 ttgtcttttg ctgcctgtta ggcgtgctgg cctgcaccct ttttgcctgg aatcgcaact
    14461 actttgtttt gctattcgtt ggcgtctttc ttagcagctt tggctcgacc gctaacccgc
    14521 aaatgtttgc ccttgcccgt gaacatgccg acaaaaccgg acgtgaggcg gtgatgttca
    14581 gctctttttt acgcgctcag gtttcactgg catgggtcat tggcccaccg ctggcttatg
    14641 cgttagcgat gggtttcagc tttacggtaa tgtatctgag cgcagcggta gcgtttattg
    14701 tttgcggcgt gatggtgtgg ctgtttttac cgtcgatgca aaaagagctt ccgctggcga
    14761 ccggcacggt tgaagcgccg cgccgtaacc gtcgcgatac gctactgctg tttgtcattt
    14821 gtacattaat gtggggctcg aacagcctgt acatcatcaa catgccgcta tttattatca
    14881 acgaactgca tcttcccgag aaactggccg gtgtgatgat ggggaccgcc gccgggctgg
    14941 aaatcccgac gatgttgatt gccggatatt tcgccaaacg tctgggtaag cgtttcttaa
    15001 tgcgcgttgc tgccgtgggt ggcgtctgtt tttacgcagg aatgctgatg gcgcattcac
    15061 ctgtcattct gttgggcttg cagctgctaa atgctatttt tattggcatt ctgggcggca
    15121 tcgggatgct ctattttcag gatctgatgc ccggtcaggc gggttcagcc accacgctct
    15181 ataccaacac ttcgcgcgtg ggctggatca tcgcaggatc agtggcgggc atcgtcgccg
    15241 agatctggaa ttatcacgct gtgttctggt ttgcgatggt gatgattatc gccactctgt
    15301 tttgcttact gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc
    15361 aggtcatggc ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac
    15421 agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa
    15481 tgcagggcgt attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag
    15541 gggcagtgca acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta
    15601 accaccggat tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata
    15661 ccacttgccc gaaaggcccg tcgcgagtac tttgtcgcga tatttttgac attttcgact
    15721 acaggaattt ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca
    15781 acggcaaact gctgctggtt aaggatattg atattcagtc gcccactgcc cgcggtgccg
    15841 ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt
    15901 tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct
    15961 atgtcgatgg caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca
    16021 ccaaagatca gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc
    16081 aggtgctgac ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg
    16141 aaatcgttga aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg
    16201 cgacattgag cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa
    16261 ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg
    16321 cagaaaaggg atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg
    16381 cgcttcctga acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc
    16441 atcgcattaa caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt
    16501 taatttatcg ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc
    16561 atcatgtaca cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg
    16621 gctttggcgc ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc
    16681 agggcggcga ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc
    16741 agctccgcgc acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg
    16801 tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa
    16861 ttattgagaa attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag
    16921 gctattgtat tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg
    16981 atctacaaac ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac
    17041 gccgccgtga gcgcggcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca
    17101 atggtcatgt ggtgaaaaac gcggtgctgg gaatggcaga aaaacgttcg ccagaactcg
    17161 ccgggtggat aaaagagcac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg
    17221 ctgcaaccga cgaatcactg gtggaaatca gccagcatct gggggtgaat gatccctgcg
    17281 cgattagctg cgaaccgttt atccagtggg tggtggaaga taacttcgtc gctgggcgtc
    17341 ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga
    17401 aactgcggat gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat
    17461 tcgcccatat cagtgattgc atgcaggatc gcgcatttcg ccatgccgcc agaacattaa
    17521 tgctggatga gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg
    17581 ataagttaat tgcacgtttt gctaatccgg cgctgaaaca taagacctgg caaatcgcga
    17641 tggatggcag ccagaaatta ccgcaacgca tgctggcagg tattcgcata catcaggggc
    17701 gcgaaacgga ctggtcgttg ctggcattag gcgttgcagg ctggatgcgt tacgtcagcg
    17761 gcgttgatga tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg
    17821 aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag
    17881 ttttcggtga tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc
    17941 aacaaatcgt acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat
    18001 ttctgcggtt aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgctt
    18061 tttttagcca ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca
    18121 ggaccaacct catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt
    18181 tagcccataa agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg
    18241 gaattgatgg tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct
    18301 gcatgtgctg cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg
    18361 gaaaaccaga ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagatcctcg
    18421 atctgttaac cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc
    18481 tcgatccgcg cttgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc
    18541 tggctgccgc agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc
    18601 aagcgctaca gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc
    18661 atgggaaagt tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg
    18721 ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc
    18781 cagagcatca acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct
    18841 ggatattcga cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg
    18901 caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc
    18961 gtcagggcga tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg
    19021 agctgatttc cagcagcgaa gctgactgga atgctttgca gagcgcgctg ttgaagcttc
    19081 gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat
    19141 gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc ggcctcgcgc tgtttctttc
    19201 ctggtatatt cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt
    19261 ctttaatcag aaactggtcg aaagtaaggc ctttttgtgg ctggttgcat tgaccaacaa
    19321 tcgcgctttc gacggttgtt ctctgctggc gatgggaatg ttgatgctga gtttctggct
    19381 gaaagaaaac gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac
    19441 tgcagtggta ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac
    19501 attgactttt accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc
    19561 ctcacgagat agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat
    19621 gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt attatttttg tggtttttgc
    19681 atttcccaga gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac
    19741 cgtgatattg atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac
    19801 cttttttgac aaatcactac cgggaaaaaa caaacatttc caaaacaaat aactcacagt
    19861 aattaacatc atcaggctta tttttatagt gaggataatc ctgatgatgc gcaccgtgct
    19921 ttcatccatc gcgcgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac
    19981 tcaatcgtac tttaattgac caatcccgct tattaacttt ctgtatcact ttttcttata
    20041 aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt
    20101 gcctcggcgg tagattatcc tcacagcata taattttgtg cgttagtcca cagatttggc
    20161 cttaaggaat tgtttcaaca tgcccaggta attagtctcg tgtcgcttgg cattttttta
    20221 taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc
    20281 gattttgaga tatatcttgc gcgggatccc cgcgattgca gtagcggttc tgctttctgc
    20341 atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcag tgggtagtga
    20401 aacatcatca ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt
    20461 aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg
    20521 cagcactaaa aaaggtatcg attgttctgg ttttgtacaa cgtacattcc gtgagcaatt
    20581 tggcttagaa cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg
    20641 cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt
    20701 cggtatttat atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat
    20761 ttccagcatg aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag
    20821 ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag
    20881 cactgcttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct
    20941 tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat
    21001 taacggaatc atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat
    21061 tgttgcaggc gtgatgattg cgatactggt gagttgcctt caatttttag tggcctggca
    21121 taagcacgaa gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta
    21181 ttttgccgac ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca
    21241 gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt
    21301 gctggtgaaa gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact
    21361 caatgaattg attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg
    21421 cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa
    21481 aaatagcggt gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc
    21541 acggcaggaa gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt
    21601 ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg aaactaaaat
    21661 tgcgggcatt cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg
    21721 gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt ggcctgctct gctattacct
    21781 gatgagcgta cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc gcgaacaatt
    21841 ttacgtggcg tatcaaccgg tggtggatac acaagctttg cgagtaacgg gcctggaagt
    21901 actgctacgc tggcggcatc ctgtcgcggg agaaattccc ccggatgcct tcattaactt
    21961 tgccgaatcg caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg
    22021 cgatgccgca gaattagaaa aagtgctgcc agtaggcgtc aaatttggta ttaacattgc
    22081 gccggaccat ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct
    22141 acccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca
    22201 agaagccaca caactcttcg cctggctgca ctcggtcggc gtagaaattg ctattgatga
    22261 cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa
    22321 aattgaccgt ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga
    22381 cgcggtgctg acgctggcga aacgtctcaa tatgctgaca gttgctgaag gggtcgaaac
    22441 gccggaacaa gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat
    22501 tagtcgcccg ttaccgctgg acgattttgt ccgctggctg aagaaaccgt atacgccgca
    22561 gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta
    22621 atgatgagta aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc
    22681 tctgttcacc tttggtgtgc aggcgcaggc tatcaaggaa agctatgcct ttgccgtgct
    22741 gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc
    22801 aaaaggtggg cagataacgt tgtcagccct cggcaccttc gatactttca accgctatgc
    22861 actgcgcggc aacccgggcg cacgcaccga gcagctgtac gacacgctat ttacgacttc
    22921 cgatgacgaa ccaggcagtt attacccgct gattgctgaa agcgcacgct atgctgacga
    22981 ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttcgcccat
    23041 tactgcccgc gatgtagagt ttacttttca aaaatttatg accgaaggcg tgccgcaatt
    23101 tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga
    23161 gttagctaaa cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga
    23221 aaagtactgg aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg
    23281 tccgtaccgc attacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga
    23341 ttactgggca gcaaacttac cggtaaaccg cggacgctgg aatttcgaca ccattcgcta
    23401 cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt
    23461 gcgtatggaa aacgacgcca aaaactgggc cacgcgttat accggtaaaa atttcgataa
    23521 aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc
    23581 gtttaatatc caacgtccag tattcagcga tcgccgggtg cgggaagcaa tcactctcgc
    23641 ctttgacttt gaatggatga acaaggcgtt gttttacaat gcctggagtc gcacgaacag
    23701 ttattttcag aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct
    23761 tctggcacca atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc
    23821 ggtatccaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa
    23881 cgaagcgggc tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccact
    23941 cagctttgaa ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca
    24001 cagcctgcaa cggctgggta tcaacatgga cattcgcaag gtggataact cgcaaatcac
    24061 taaccgcatg cgcagtcgcg actatgacat gatgccgcgc gtatggcggg cgatgccgtg
    24121 gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc
    24181 ccccggcgtg caaagcccgg ttatcgactc gctgatcaac caaattattg ccgcgcaggg
    24241 aaataaagaa aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta
    24301 ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc
    24361 ccagccggcc gtgcgcccca tctatagcct cggtatcgat acctggtggt atgacgtcaa
    24421 taaagcggcc aaactgccgt ccgccagcaa acagggagag tagatgggcg cttacctgat
    24481 tcgccgtctg ttgctggtga tcccaacatt atgggcgatt atcaccatca actttttcat
    24541 cgtgcaaatt gcgcctggcg gtccggtcga ccaggccatc gccgccattg agtttggtaa
    24601 tgccggagta ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg
    24661 tgtcggcaat atcagcgaca gtaagtaccg tggcggacgc ggattagatc cagaagtgat
    24721 cgctgagatc actcatcgct acggttttga taagccgatc cacgaacgtt acttcaaaat
    24781 gctctgggac tacatccgct ttgattttgg cgatagcctg tttcgcagcg cctcggtgct
    24841 gacgctgatt aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat
    24901 tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagccgctt
    24961 tgacgtctgg agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc
    25021 catcctgctg attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg
    25081 cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg
    25141 gcatatcacg ctgccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct
    25201 gacaaaaaac tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa
    25261 aggggtaagt gaaaaaaata ttctctggaa acatgtgttc cgcaacgcca tgctgctggt
    25321 gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga
    25381 ggtgatgttt tcactcaatg gtctgggctt actgggctac gaagcgaccg tctcgcgcga
    25441 ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat
    25501 tgtcagtgat atcagctata cgctggtcga tccgcgtata gattttgagg gacgttaatg
    25561 tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt ttcgccataa ccgtcgcggc
    25621 tactggtcgt tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc
    25681 gccaacgata aaccgttgct ggtacgttat gacggcagtt ggtatttccc gttgttgaaa
    25741 aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg
    25801 tggctgaaac aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt
    25861 gctaccagta tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac
    25921 tggctgggaa cggatgccaa cggcggcgat gtgctggcac gtattctcta tggcacgcgg
    25981 atctcggttc tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg
    26041 ggggcgctac aaggctatta cggcggtaaa gtcgatctct gggggcaacg ctttattgaa
    26101 gtatggtcgg ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct
    26161 aacttctggt ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg
    26221 gtgcgggcgg agtttttacg cacccgtaat ttcgactaca ttcgtgcggc gcaggcgctt
    26281 ggcgtcagcg atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc
    26341 ctcacctttt taccgtttat tttatgtagt tcgataacca ccctgacctc actcgatttc
    26401 ctcggcttcg gtctgccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa
    26461 aataaccttc aggctccgtg gcttgggatc accgccttct tgtcggtggc gatattattg
    26521 tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg
    26581 tagcatgacg caaactctgt tagcgattga agatttgtcg gtgggttttc gccatcagca
    26641 aaccgtacgt acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc
    26701 gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct
    26761 cccttccccg ccggttgaat atctctccgg cgatattcgt tttcgtggcg aatcgctgct
    26821 tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca
    26881 agagccgatg gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct
    26941 ttcactccac cgcgggatgc gtcgggaagc ggctcgaggc gaaattctta actgccttga
    27001 tcgcgttggt atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg
    27061 cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat
    27121 tgccgatgaa ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt
    27181 gcgcgaactg caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat
    27241 tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa
    27301 taacgccgct acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag
    27361 tgaaccgtca ggcgacccgg tgccgttgcc agaacctgcc tcaacgttgc tggatgttga
    27421 acagcttcag gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa
    27481 tgtggtggtg aaaaacatca gttttacgct acgagcgggt gaaacactgg gtttagtggg
    27541 cgagtccggt tccgggaaaa gtacgacggg actggcgctg ctgcgactga ttaattctca
    27601 gggcagcatc atctttgacg gtcagccact gcaaaattta aatcgccgcc agctgttacc
    27661 tattcgtcat cgcattcagg tggtatttca ggatccaaac tcctcgctca acccacgact
    27721 caacgttttg cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc
    27781 acaacgcgaa caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg
    27841 ccaccgttat ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc
    27901 attaattctt aagccctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac
    27961 ggtacaggcg caaatattga cgctattgaa atcattgcaa caaaagcatc aactggccta
    28021 tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact
    28081 gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca
    28141 ggagtatacg cgtcagctac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa
    28201 agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct
    28261 ggcgatttac aaacaggcac ggctcacctt cacactccag caccgaacat ggaacttcga
    28321 tgtcgcttaa cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc
    28381 taagcaattt aagagccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt
    28441 caaccttcga acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga
    28501 ttgagagttg taatgaagtc acttattttc cccggaagca cattcacttc acaatttgtt
    28561 tacatcaatt ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc
    28621 ctcgcctgcg tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt
    28681 gcggggctat atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg
    28741 actttttaca taaattgatt ttacataaaa taaacatata tcgggggaat gatagatttg
    28801 tggttgacat gcatcaacaa aggaagcctt ttagcttcct cgttgtgcaa tagatcaccg
    28861 ttttttcggt cgactggcgt acagacagaa gagaatggag ctgcttgcgc agaatgcaat
    28921 tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat
    28981 tgcccctatg ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg
    29041 aaactcatca agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa
    29101 cgccgcaacg ccaaccacca gcgaccagaa tcccagcccc agcagcgcac tgatgaccat
    29161 ccacgctgcc ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat
    29221 gcggcgaacg aagcggctgt taaaaatggt catcacgaac aggaaaacaa tgtttagtgc
    29281 aaagtaataa ccaaagtttt ccggcgcgac gtggttaatt tcaatgtaga caaacggtcc
    29341 ggcgcttaag aacgagaaca tcccggcaaa gctgaaacca ctggcaagca tataactcag
    29401 gacacgctta tgacggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaaagg
    29461 ctggcgacgc tccggtggta aggtttcttt aatcaggaag aaaatcattg ccgaagccag
    29521 aatcgccgct aatgccagga tccagaagat gtaatgccag ctcagccaca ccagcaccca
    29581 accgccgaca atcggtgcca tcagcggtgc aatagtggtc accagcatga caaacgacat
    29641 catccgcgag aactcttctt tcgggtaaat atcgcgcatc agggcgttaa tgaccacgct
    29701 ggccgcagcc gccgccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtgtt
    29761 tgccaacgca cacgccaccg cggcggcggc aaacaccagc gtaccgccga gcaccaccgg
    29821 cttacgcccg aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag
    29881 aatataagta ctgagggtca tctgcgtact gcccgcaggt acgccaaact gcgctgaaat
    29941 taccggtagc gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc
    30001 aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa
    30061 gtcatggaaa agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc
    30121 gggggctaaa tcagcatcca gcgtaatacc gccaatacgt tcacgatgca gctcaaccac
    30181 gtggttaccc acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt
    30241 cagacgaacc tgcgttgggg taatcacttc cagcaccgca ggcttagtga gatctttttc
    30301 gttatgcagc tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta caggtgattc
    30361 cagtgtcacc agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca
    30421 ctgaccatca tcagtcatca gcaccagacc ggtggtatcg atatccagac gtcctgcggc
    30481 gtgcagtttc caggctaccg gttcatccag aaaatagagc accgttgggt gatcagggtc
    30541 gtccgtggag caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccatgttg
    30601 ctgcgccagc gggttgccat cgtaagcgac atcatgttca ggaagcagtt tgaacgctgc
    30661 attacgaaca atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat
    30721 agcacggcta acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt
    30781 gcctgtaaaa acggaggtcg ggcattgacc cgaaaatctg aactgttgtc tgcccagtat
    30841 agcggtctaa taacatccct caagggaaaa agatccatgg catactatta gcagaataat
    30901 ctacctacgc gagaccatga tttttacact ccgcccatat cagcaagaag ccgtggatgc
    30961 cacgctcaac cattttcgtc gtcataaaac ccctgccgtt atcgtgctgc ccaccggcgc
    31021 aggtaaaagc ctggtgattg ccgaactggc acggctggcg cgtggtcgtg ttctggtgct
    31081 ggcacacgtc aaagaactgg tggcgcaaaa ccatgcaaag tatcaggcgc tggggctgga
    31141 agccgatatt tttgccgctg ggttaaagcg caaagagagc cacggtaaag tggtatttgg
    31201 cagcgtgcag tcggtcgctc gtaatcttga tgcctttcag ggtgaatttt cgctgttgat
    31261 tgtcgatgaa tgccaccgta ttggtgacga tgaagagagc cagtatcagc aaatcctcac
    31321 tcacctgact aaagtgaatc cccacttacg cctgctgggg ctgactgcca cgccttttcg
    31381 attgggcaaa ggctggatct accagttcca ttatcacggc atggtacgcg gcaatgagaa
    31441 agcccttttc cgtgactgca tttatgagct gccgctgcgt tatatgatta aacacggcta
    31501 tctgacgccg ccagaacgac tggatatgcc agtagtgcaa tacgatttca gccgcttgca
    31561 ggcacagagt aacgggctgt tcagcgaagc tgatctcaac cgtgagctga aaaaacagca
    31621 acgcattacg ccacatatca tcagccagat catggagttc gccgaaaagc gcaaaggggt
    31681 aatgattttc gccgccaccg ttgaacacgc aaaagagatt gtgggattac tgcctgccga
    31741 agatgctgca ctgattactg gcgacacccc cggcgctgag cgcgatgtgt taattgaaga
    31801 ttttaaagcc cagcgttttc gctatctggt caacgtcgca gtactgacca ccggatttga
    31861 cgccccgcac gtcgatctta tcgccattct gcgccctacc gaatcagtga gtctttacca
    31921 acaaattgtc gggcgcggtc tgcgtctcgc tccgggcaag actgattgtt taattcttga
    31981 ttatgcgggt aatcctcacg atctctacgc gccggaagtt ggtacaccga aaggcaaaag
    32041 tgacaacgtt ccggtacagg ttttctgccc tgcctgcggt tttgccaaca ccttttgggg
    32101 gaaaacgacc gccgacggga cattgattga acactttggt cgccgctgtc agggatggtt
    32161 tgaagatgac gacggtcatc gcgaacagtg tgacttccgt ttccgtttta aaaattgccc
    32221 gcaatgtaac gcagaaaatg atattgccgc tcgccgctgc cgcgaatgtg acacggtact
    32281 ggttgatccg gatgatatgt taaaagcggc gctacgactg aaagatgcgc tggtattacg
    32341 ctgtagcggc atgtctttgc agcatgggca cgacgagaaa ggcgaatggt tgaaaatcac
    32401 ctattacgat gaagacggcg cggatgtgag tgagcgtttc cgtctgcaaa cgcccgccca
    32461 gcgaactgcc ttcgagcagc tttttatccg cccgcatacg cgcacaccgg gcatccctct
    32521 gcgctggatc accgccgccg atatcctcgc ccagcaagcc ttactgcgac atccggattt
    32581 tgtcgtcgcc cgcatgaaag gtcagtactg gcaggtgcgt gaaaaagtgt tcgattacga
    32641 aggtcgtttt cgtcgggcgc acgaattacg cggttaatgg tagttttgat tgatgtataa
    32701 ggcgattgag tatagaatct cgcccgcttt tgcatacgca aagcagatca cttacctgtt
    32761 gctgggtcgc ctgtagcagg attaatttaa gagagaaaga aatgtttact atcaacgcag
    32821 aagtacgtaa agagcagggt aagggtgcga gccgccgcct gcgtgccgct aacaagttcc
    32881 cggcaatcat ctacggtggc aaagaagcac cgctggctat cgagctggat cacgacaaag
    32941 tcatgaacat gcaagctaaa gctgaattct acagcgaagt tctgaccatc gttgttgacg
    33001 gtaaagaaat caaagttaaa gctcaggacg tacagcgtca cccgtacaaa ccgaagctgc
    33061 agcacatcga cttcgttcgc gcttaattgc tgaataagtt gtaaaaaacc ccgctccggc
    33121 ggggtttttt gtatctgcag attatgcctg attacggtat tgctattttt tgcaggccag
    33181 ataaggcgtt cacgccgcat ctggcattca gaacacaaaa ccgattaatt gccgccagac
    33241 gtccggcgct gcaattggtc gcgcaaattc ggcggcgtgc ctttaatggt caaggtatcg
    33301 gttgctggat cccagaagat gcgttcacct aacagcatcg catcaaagtt aatagtcaag
    33361 ccaccgccgc taccggcaaa cttcgtcaac tgacgcagtg tgctacggtc tgccgggaag
    33421 ctctcttcca gttcgtagcc tttttcagcc gcaaactccg tgaagctcac ttcgcttacg
    33481 cctgccagct ctttcgacag cgattccagt tcgatctctt cgcctgcctg cagctgctca
    33541 ttacagtagc tataaacctg ctggcgcacg ttctgtcgct ctgctttatc cagttgtgct
    33601 tcggcagtga aatcatcaac cgcttgcaac agcccacggt tttgcgcttt cgcgttcaat
    33661 ccttcgctgg ccccgaggaa atccataaag aaatccgcca ctttgcgccc tactcgccct
    33721 ttcaggaaag tgagatagcg ggtggactct ggattggttt cccattcggt taaatcgata
    33781 cgcgcaacaa tatccgcatg attgatatca agataatggg ttgggttgat gtcgagattt
    33841 tcgttaacgc gcatactgct caggttgctc agaactgcca ccagcagata ctccaccgcc
    33901 agataacggt agtggcaaaa cagcacgaat ccaccgtcag caaaaggata tttcgctaat
    33961 tcgtcacgca ggcgcccggt tgccgcgcgg ctaaatgcca gaaagtcctc ttcgccctga
    34021 cgctgtaagc gcagcgtctg cgccagttca ctctcttcgc taaacaagcc gtaagcttta
    34081 tttttggcgc tatagacccg atgcagttct gccaccatct caacgacggt ttctgtcggt
    34141 tccagcaatg aatcgcgcaa caccagctca aggttctgct catcacgctt gataagctgg
    34201 tgcagggcaa tctggttgat atccagactc atgataaact ctcctttaag accgggcggt
    34261 attcaaccac cgccggttaa gcgacgcaat aaaagatggc ggtcatttta cagaaggcag
    34321 cgtaacgaga atgatttaac gcataaaaaa gcagaaaaaa aaccgttgct acggtaatat
    34381 gttgcccttt catcaactaa ctgatttcga tttatgccac aaatttcccg ctacagtgat
    34441 gaacaggttg aacaattgct cgcagagctg ctcaacgtac tggaaaaaca taaggctccg
    34501 accgaccttt ccctgatggt gttaggcaac atggtcacta accttatcaa caccagcatt
    34561 gccccggccc aacgccaggc aattgccaac tcttttgccc gcgccttaca gtcctctatc
    34621 aacgaagaca aagcgcacta agggaaacag ataactggtt atggtaactc atcgtcagcg
    34681 ctaccgtgaa aaagtctccc agatggtcag ttgggggcac tggtttgcac tgttcaatat
    34741 tctgctttcg ctcgtcattg gcagccgtta cctgtttatc gccgactggc cgacaacgct
    34801 tgctggtcgc atttattcct acgtaagcat tatcggccat ttcagcttcc tggtgttcgc
    34861 cacctacttg ctgatcctct tcccgctgac ctttatcgtc ggctcccaga ggctgatgag
    34921 gtttttgtcc gtcattctgg caacggcggg aatgacgcta ttactgatcg atagcgaagt
    34981 ctttactcgt ttccatctcc atcttaatcc catcgtctgg caactggtta tcaacccaga
    35041 cgaaaatgag atggcgcgcg actggcagct gatgttcatc agcgtgccgg ttattttatt
    35101 gcttgaactg gtgtttgcga cgtggagctg gcaaaagctg cgcagcctga cgcgtcgtcg
    35161 acgcttcgcg cgcccgctgg ccgcattctt atttatcgcc tttatcgcct cgcatgtggt
    35221 gtatatctgg gccgatgcca acttctatcg cccgatcacc atgcagcgcg ctaacctgcc
    35281 gctttcgtac ccgatgacgg cgcgacgttt tcttgagaag catggtctgc ttgatgcgca
    35341 ggagtatcaa cgccgtctta ttgagcaagg taatccagac gccgtttccg tacagtatcc
    35401 gttaagcgaa ctgcgctatc gcgatatggg caccgggcag aatgtgctgt tgattactgt
    35461 cgatggcctg aactactcac gcttcgagaa gcagatgcct gcgctggcag gttttgctga
    35521 gcaaaatatt tcgttcacgc gccatatgag ctccggcaac actacagaca acggcatctt
    35581 tggcctgttc tatggcatct cgccgagcta tatggacggc attctgtcga cccgtacgcc
    35641 tgcggcatta attactgcgc ttaatcagca aggctatcag ctggggttat tctcgtcaga
    35701 tggctttacc agcccgctgt atcgccaggc attgttgtca gatttctcga tgccgagcgt
    35761 acgcacccaa tccgacgagc agaccgccac gcagtggatc aactggctgg gccgctacgc
    35821 acaagaagat aaccgctggt tctcgtgggt ctctttcaat ggcactaaca ttgacgacag
    35881 caatcagcag gcatttgcac ggaaatatag ccgggcggca ggcaatgtcg atgaccagat
    35941 caaccgcgtg ctcaatgcac tgcgtgattc tggcaaactg gacaatacgg ttgtgattat
    36001 cactgccggt cggggtattc cgctgagcga agacgaagaa acctttgact ggtcccacgg
    36061 tcatctgcag gtgccattag tgattcactg gccaggcacg ccggcgcagc gtattaatgc
    36121 gctgactgat cataccgatc tgatgacgac gctgatgcaa cgcctgctac atgtcagcac
    36181 acctgccagc gaatattcgc aaggtcagga tttgttcaac cctcaacgcc gtcattactg
    36241 ggttactgca gcggataacg atacgctggc aattaccacc ccgaaaaaga cgctggtgct
    36301 gaacaataac ggtaaatacc gcacttacaa cttacgtggt gaaagagtga aagatgaaaa
    36361 accacagtta agtttgttat tgcaagtgct gacagacgag aagcgtttta tcgctaactg
    36421 attaattata aatcagttag cgaaatatct tacttgcaat cggtgtggaa aacggtagta
    36481 ttagcagcca cgagtcggca cgtagcgcag cctggtagcg caccgtcatg gggtgtcggg
    36541 ggtcggaggt tcaaatcctc tcgtgccgac caaatcgccc accggatatc accggaaagc
    36601 accggataag acagggataa cccgctaatc acttgattgg cgggtttttt tatgtttttt
    36661 agtcaccgaa catcaccgga tttataagct atcagccgga ttttttagtt atacgtttag
    36721 ttatacgggc tggcgtataa ctaaagccgt ataactaaaa ccctgttttt tcgtgtggaa
    36781 tcatagacat atggcgagaa aaacacaccc attaaccaca gtgcagatca aagcagccag
    36841 accagcgcaa aaggagtaca ccctgcagga cggcggaggg ctttttctcc tggtcaaacc
    36901 gtctggatca aaactctgga gattttccta ctaccgacca tcggacaaaa aaagaatatt
    36961 gctgagtttt ggatcgcttg aagatgtttc cctggctgat gccagaaaac gccgtagcga
    37021 gtacaggacg ttaatcagtg ccggaactga cccgcaggac cacgagaggc aaaaaagaga
    37081 gacagaggcc cgaagacaag ggaacacgtt cgaaaatgtg gcggcggcat ggtaccaggt
    37141 gaaaatcagc cagaatctgg cccccaacac gattaaagac atctggcgtt cgctggataa
    37201 atatgtattc ccgttcatcg gcaacacgcc aatagatacc ctcaccgccc gaaggttcgt
    37261 tgaagtgctt acccccatca aggagcgcgg caacctggaa acactcaagc gggttttaca
    37321 gcgcgttaat gaggtaatgg attacgccgc caacagtggg ctgattgatg ccaatccggc
    37381 tatgaatgtg cgtaaggcgt ttccctcacc tgtaaaaaaa catatgccaa caatccgccc
    37441 cgaacagctg cccgagctta tgcaggcttt atcagtatcg gcaacagaac ggcagaccag
    37501 attactgatt gaatggcagt tactgaccgt aacccgtccc gccgaagcgt catcaacgcg
    37561 gtgggatgaa atcaacctgg acgcgaagca atggacgata cctgccggac gcatgaagat
    37621 gcgcagggat cacgttatcc cgctttccgg tcaggctatg gcggtgctgg aggccatgaa
    37681 accaatcagc caccaccgca attacgtttt cccaagtctg aaagacccac agcagccgat
    37741 gaacagccag acagctaacg cagcattgcg gcgtatggga ttcgctggcg tgctggtgtc
    37801 tcatggatta cgcgccatat tcagcacagc agcgaacgag gaaggattcg agccggacgt
    37861 aatagaggcg gcacttgccc acgtcgacac caacgaagtt agacgggcat acaaccggag
    37921 caactacata gaaaaacgca tcgtgctgat gcgctggtgg ggcgaatttg tcgaggctgc
    37981 ggcgacgggc gtaaccctcg ccagtggtaa aaggggtatc cgagccgtgt agctgtacag
    38041 aaaaccagta aaaactacga aaaccatgta aaaccgtcgt ataattgcat caaatttaac
    38101 gacaaggccg tgaaacatga aaccgttaag atgcaaaaaa atatcagatg caattgcgac
    38161 gggctgcaac tggccctgat ggttcagcat gaattttggt caacctacga tccggaggac
    38221 agaacgacgg ccccatcaaa aaaacaggta gtagattttc tggtatcccg tggcgcttcc
    38281 agaaatctgg cggtaagtat tgataaggtc gtacgtccgg catctatgaa gatcggaggc
    38341 aggcccaaaa aatggcggta acaatcctgg aagcggcaga aatgccgctt tttttataat
    38401 tccatttcaa atcatcaata taaaaaacgg tgtataccgt ttaaaaacgg tgggaactgt
    38461 ttttacccat atccgatgat ttaccgtatt tgtcaccgga atacaccgga ttcacaaggt
    38521 aaatcacgat ggaagcaatc agaaaaatta tctttcgcca ggaagtaaaa aaaattatcc
    38581 acatcaaggc agacagcacg ctgcaaagca tgatcaacgc cggagaattt ccgcagggtt
    38641 ttcgcgttgg tttacgccgt cgcggatggt atgaggatga tgtgttgtcc tggcagaaag
    38701 aacgcgaaga ggaagcacgc ggaacggctg cttaacgggt atcacagata tgacaaacac
    38761 gaaaaaagcg gccccgatat ggagccgcct ttctgaacaa ttaacccgct gcgccttatg
    38821 tgtatgtgat cccaaacata agcacgggga tgatagccgc tatcaggctg gtgggcaatg
    38881 caatcagtct ggttcagttc gttgccatac ctgcaatgag cgcttttccc tgtactcttt
    38941 aaggaattgc tcaagggcaa aagcacatgg cgcgaatctt tctgattcat gctctatctt
    39001 tctgcgccgt cttttccgtg ccggtgataa tgttttggtc aattctttat cggtcattgt
    39061 gttgtcctgc a
//
LOCUS       DAZFWL020000032        38257 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFWL020000032 DAZFWL020000000
VERSION     DAZFWL020000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 38257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.032"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..354
                     /locus_tag="ACHKNP_RS08855"
                     /old_locus_tag="pgaptmp_001771"
     CDS             <1..354
                     /locus_tag="ACHKNP_RS08855"
                     /old_locus_tag="pgaptmp_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HGU4703957.1"
                     /translation="NGTEIAITYVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEP
                     LSAKYKNIAGVEEKLTYTDTYAQENVTIDMEKVDFKALQGISGINVSAEDAKKGITMA
                     QMELVMKAAGFKEVK"
     gene            complement(394..864)
                     /gene="yehS"
                     /locus_tag="ACHKNP_RS08860"
                     /old_locus_tag="pgaptmp_001772"
     CDS             complement(394..864)
                     /gene="yehS"
                     /locus_tag="ACHKNP_RS08860"
                     /old_locus_tag="pgaptmp_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HGU4703958.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIFLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(911..1630)
                     /gene="btsR"
                     /locus_tag="ACHKNP_RS08865"
                     /old_locus_tag="pgaptmp_001773"
                     /gene_synonym="yehT"
     CDS             complement(911..1630)
                     /gene="btsR"
                     /locus_tag="ACHKNP_RS08865"
                     /old_locus_tag="pgaptmp_001773"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HGU4703959.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1627..3312)
                     /gene="btsS"
                     /locus_tag="ACHKNP_RS08870"
                     /old_locus_tag="pgaptmp_001774"
     CDS             complement(1627..3312)
                     /gene="btsS"
                     /locus_tag="ACHKNP_RS08870"
                     /old_locus_tag="pgaptmp_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HGU4703960.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3534..4265
                     /gene="mlrA"
                     /locus_tag="ACHKNP_RS08875"
                     /old_locus_tag="pgaptmp_001775"
     CDS             3534..4265
                     /gene="mlrA"
                     /locus_tag="ACHKNP_RS08875"
                     /old_locus_tag="pgaptmp_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HGU4703961.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            4325..4432
                     /gene="yohO"
                     /locus_tag="ACHKNP_RS08880"
                     /old_locus_tag="pgaptmp_001776"
     CDS             4325..4432
                     /gene="yohO"
                     /locus_tag="ACHKNP_RS08880"
                     /old_locus_tag="pgaptmp_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HGU4703962.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(4413..5144)
                     /gene="yehW"
                     /locus_tag="ACHKNP_RS08885"
                     /old_locus_tag="pgaptmp_001777"
     CDS             complement(4413..5144)
                     /gene="yehW"
                     /locus_tag="ACHKNP_RS08885"
                     /old_locus_tag="pgaptmp_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HGU4703963.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(5149..6075)
                     /gene="yehX"
                     /locus_tag="ACHKNP_RS08890"
                     /old_locus_tag="pgaptmp_001778"
     CDS             complement(5149..6075)
                     /gene="yehX"
                     /locus_tag="ACHKNP_RS08890"
                     /old_locus_tag="pgaptmp_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HGU4703964.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(6068..7225)
                     /gene="yehY"
                     /locus_tag="ACHKNP_RS08895"
                     /old_locus_tag="pgaptmp_001779"
     CDS             complement(6068..7225)
                     /gene="yehY"
                     /locus_tag="ACHKNP_RS08895"
                     /old_locus_tag="pgaptmp_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HGU4703965.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(7232..8149)
                     /gene="osmF"
                     /locus_tag="ACHKNP_RS08900"
                     /old_locus_tag="pgaptmp_001780"
     CDS             complement(7232..8149)
                     /gene="osmF"
                     /locus_tag="ACHKNP_RS08900"
                     /old_locus_tag="pgaptmp_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HGU4703966.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGSVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(8360..10657)
                     /gene="bglX"
                     /locus_tag="ACHKNP_RS08905"
                     /old_locus_tag="pgaptmp_001781"
     CDS             complement(8360..10657)
                     /gene="bglX"
                     /locus_tag="ACHKNP_RS08905"
                     /old_locus_tag="pgaptmp_001781"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HGU4703967.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            10853..12568
                     /gene="dld"
                     /locus_tag="ACHKNP_RS08910"
                     /old_locus_tag="pgaptmp_001782"
     CDS             10853..12568
                     /gene="dld"
                     /locus_tag="ACHKNP_RS08910"
                     /old_locus_tag="pgaptmp_001782"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU4703968.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(12606..13538)
                     /gene="pbpG"
                     /locus_tag="ACHKNP_RS08915"
                     /old_locus_tag="pgaptmp_001783"
     CDS             complement(12606..13538)
                     /gene="pbpG"
                     /locus_tag="ACHKNP_RS08915"
                     /old_locus_tag="pgaptmp_001783"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HGU4703969.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(13712..14299)
                     /gene="yohC"
                     /locus_tag="ACHKNP_RS08920"
                     /old_locus_tag="pgaptmp_001784"
     CDS             complement(13712..14299)
                     /gene="yohC"
                     /locus_tag="ACHKNP_RS08920"
                     /old_locus_tag="pgaptmp_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HGU4703970.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14469..15047
                     /gene="yohD"
                     /locus_tag="ACHKNP_RS08925"
                     /old_locus_tag="pgaptmp_001785"
     CDS             14469..15047
                     /gene="yohD"
                     /locus_tag="ACHKNP_RS08925"
                     /old_locus_tag="pgaptmp_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4703971.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(15177..15938)
                     /gene="yohF"
                     /locus_tag="ACHKNP_RS08930"
                     /old_locus_tag="pgaptmp_001786"
     CDS             complement(15177..15938)
                     /gene="yohF"
                     /locus_tag="ACHKNP_RS08930"
                     /old_locus_tag="pgaptmp_001786"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4703972.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(15991..17427)
                     /gene="mdtQ"
                     /locus_tag="ACHKNP_RS08935"
                     /old_locus_tag="pgaptmp_001787"
     CDS             complement(15991..17427)
                     /gene="mdtQ"
                     /locus_tag="ACHKNP_RS08935"
                     /old_locus_tag="pgaptmp_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HGU4703973.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADVQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            17651..17734
                     /gene="yohP"
                     /locus_tag="ACHKNP_RS08940"
                     /old_locus_tag="pgaptmp_001788"
     CDS             17651..17734
                     /gene="yohP"
                     /locus_tag="ACHKNP_RS08940"
                     /old_locus_tag="pgaptmp_001788"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HGU4703974.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(18122..19072)
                     /gene="dusC"
                     /locus_tag="ACHKNP_RS08945"
                     /old_locus_tag="pgaptmp_001789"
     CDS             complement(18122..19072)
                     /gene="dusC"
                     /locus_tag="ACHKNP_RS08945"
                     /old_locus_tag="pgaptmp_001789"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HGU4703975.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            19311..19709
                     /gene="yohJ"
                     /locus_tag="ACHKNP_RS08950"
                     /old_locus_tag="pgaptmp_001790"
     CDS             19311..19709
                     /gene="yohJ"
                     /locus_tag="ACHKNP_RS08950"
                     /old_locus_tag="pgaptmp_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HGU4703976.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19706..20401
                     /gene="yohK"
                     /locus_tag="ACHKNP_RS08955"
                     /old_locus_tag="pgaptmp_001791"
     CDS             19706..20401
                     /gene="yohK"
                     /locus_tag="ACHKNP_RS08955"
                     /old_locus_tag="pgaptmp_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HGU4703977.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            20531..21415
                     /gene="cdd"
                     /locus_tag="ACHKNP_RS08960"
                     /old_locus_tag="pgaptmp_001792"
     CDS             20531..21415
                     /gene="cdd"
                     /locus_tag="ACHKNP_RS08960"
                     /old_locus_tag="pgaptmp_001792"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGU4703978.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            21565..22284
                     /gene="sanA"
                     /locus_tag="ACHKNP_RS08965"
                     /old_locus_tag="pgaptmp_001793"
     CDS             21565..22284
                     /gene="sanA"
                     /locus_tag="ACHKNP_RS08965"
                     /old_locus_tag="pgaptmp_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HGU4703979.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            22287..22526
                     /gene="yeiS"
                     /locus_tag="ACHKNP_RS08970"
                     /old_locus_tag="pgaptmp_001794"
     CDS             22287..22526
                     /gene="yeiS"
                     /locus_tag="ACHKNP_RS08970"
                     /old_locus_tag="pgaptmp_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HGU4703980.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            22845..24083
                     /gene="preT"
                     /locus_tag="ACHKNP_RS08975"
                     /old_locus_tag="pgaptmp_001795"
     CDS             22845..24083
                     /gene="preT"
                     /locus_tag="ACHKNP_RS08975"
                     /old_locus_tag="pgaptmp_001795"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HGU4703981.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            24077..25312
                     /gene="preA"
                     /locus_tag="ACHKNP_RS08980"
                     /old_locus_tag="pgaptmp_001796"
     CDS             24077..25312
                     /gene="preA"
                     /locus_tag="ACHKNP_RS08980"
                     /old_locus_tag="pgaptmp_001796"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HGU4703982.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(25383..26393)
                     /gene="mglC"
                     /locus_tag="ACHKNP_RS08985"
                     /old_locus_tag="pgaptmp_001797"
     CDS             complement(25383..26393)
                     /gene="mglC"
                     /locus_tag="ACHKNP_RS08985"
                     /old_locus_tag="pgaptmp_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HGU4703983.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(26409..27929)
                     /gene="mglA"
                     /locus_tag="ACHKNP_RS08990"
                     /old_locus_tag="pgaptmp_001798"
     CDS             complement(26409..27929)
                     /gene="mglA"
                     /locus_tag="ACHKNP_RS08990"
                     /old_locus_tag="pgaptmp_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HGU4703984.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDISFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(27990..28988)
                     /gene="mglB"
                     /locus_tag="ACHKNP_RS08995"
                     /old_locus_tag="pgaptmp_001799"
     CDS             complement(27990..28988)
                     /gene="mglB"
                     /locus_tag="ACHKNP_RS08995"
                     /old_locus_tag="pgaptmp_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HGU4703985.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(29268..30308)
                     /gene="galS"
                     /locus_tag="ACHKNP_RS09000"
                     /old_locus_tag="pgaptmp_001800"
     CDS             complement(29268..30308)
                     /gene="galS"
                     /locus_tag="ACHKNP_RS09000"
                     /old_locus_tag="pgaptmp_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HGU4703986.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            complement(30450..31607)
                     /gene="yeiB"
                     /locus_tag="ACHKNP_RS09005"
                     /old_locus_tag="pgaptmp_001801"
     CDS             complement(30450..31607)
                     /gene="yeiB"
                     /locus_tag="ACHKNP_RS09005"
                     /old_locus_tag="pgaptmp_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HGU4703987.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIAQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(31624..32292)
                     /gene="folE"
                     /locus_tag="ACHKNP_RS09010"
                     /old_locus_tag="pgaptmp_001802"
     CDS             complement(31624..32292)
                     /gene="folE"
                     /locus_tag="ACHKNP_RS09010"
                     /old_locus_tag="pgaptmp_001802"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGU4703988.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            32550..33386
                     /gene="yeiG"
                     /locus_tag="ACHKNP_RS09015"
                     /old_locus_tag="pgaptmp_001803"
     CDS             32550..33386
                     /gene="yeiG"
                     /locus_tag="ACHKNP_RS09015"
                     /old_locus_tag="pgaptmp_001803"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU4703989.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTIKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(33418..35409)
                     /gene="cirA"
                     /locus_tag="ACHKNP_RS09020"
                     /old_locus_tag="pgaptmp_001804"
     CDS             complement(33418..35409)
                     /gene="cirA"
                     /locus_tag="ACHKNP_RS09020"
                     /old_locus_tag="pgaptmp_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HGU4703990.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(35702..37171)
                     /gene="lysP"
                     /locus_tag="ACHKNP_RS09025"
                     /old_locus_tag="pgaptmp_001805"
     CDS             complement(35702..37171)
                     /gene="lysP"
                     /locus_tag="ACHKNP_RS09025"
                     /old_locus_tag="pgaptmp_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HGU4703991.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(37376..38257)
                     /gene="yieE"
                     /locus_tag="ACHKNP_RS09030"
                     /old_locus_tag="pgaptmp_001806"
     CDS             complement(37376..38257)
                     /gene="yieE"
                     /locus_tag="ACHKNP_RS09030"
                     /old_locus_tag="pgaptmp_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HGU4703992.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIKDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
BASE COUNT         9370 a        10204 c         9530 g         9153 t
ORIGIN      
        1 aacggcactg aaattgccat tacctatgtc tacaaaggtg acaaggtgct taagcaatct
       61 tctgaaacca aaattcaatt tgcttctatt ggtgcaacca ccaaagaaga cgctgccaag
      121 acacttgagc cgttaagcgc caaatacaaa aacatcgcgg gtgttgaaga aaaattaacc
      181 tatacagata cctacgcgca ggaaaacgtg actatcgata tggaaaaagt ggattttaaa
      241 gccctgcagg gtatttcagg aatcaacgtt tctgctgaag atgccaaaaa aggtatcact
      301 atggcgcaaa tggaactggt gatgaaagcc gctggtttta aagaagtgaa ataatcggtt
      361 ggcggtcatg ctctaaacat gaccgccaat tttttagcct tttttcacat gctggcgcgc
      421 tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg cattcgcgga agtttttatg
      481 atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa acgcggaact gctgttcggt
      541 gaggatagcc agaatgtcat cggttttcag cgaaaacgcg atgcgtaatt ttttcagcac
      601 gatgttgtta ttaatgcgac gttccggctc cagtgccgga gcagactcat ccttgccgcg
      661 tttttcataa atcaggccat tgaggaatga cgacaaaaaa atgtccggac aacgctgaaa
      721 accctcttcg tcttctttac gtagccagac ggcgatctgt tccgcggtgg cttcgacatt
      781 acccagcgcc agaatacgca ccaggtcatt attattggct ttcaaaatgt agcgcacgct
      841 gcgcagaata tcgttactta gcatgaggcc ttcaggtgtt gatgaggcaa aaagccattt
      901 tagcagtctt ttacaggcca atcgcctctt ttaagctttt cagataacgg cggctgaccg
      961 gcacggttaa gccattacgc aaaatcaact cggcctggcc gttatcttcc agacgaatct
     1021 cctgtaaatg cgcgaggtta accagatact gacgatggca gcgcagtagt ggtgtacgac
     1081 tttccagggt acgtaatgtc aattcggtaa agccctcttt cccttcgtgg ctggtaacgt
     1141 agacaccgct catccgactg ctgacaaatg ccacatcttt catttgcagc aaataaatcc
     1201 gactatgccc cgtacaaggg ataaatttca gcgcctgttg attttccggt aacagcgaaa
     1261 catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt tttctccagt cgcgcttcat
     1321 caattggctt cagcagataa tcaaaggcat gttcttcaaa ggctttaata gcgtattcgt
     1381 caaacgcagt gagaaaaaca atatacgggc gatgttccgg gtcaagcatc cccaccattt
     1441 ccagaccact gatgcgcggc atctggatat cgagaaacag cacatccggg cgcagtttat
     1501 gcaccgcgcc gatcccttcc acggcgtttg aacactctcc aacgatttca atatcgctct
     1561 gctcctgcaa aaatacgcgc aggttctccc gtgctaacgg ttcatcatcg acaattaaga
     1621 ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt attcgggtgt aactatcagg
     1681 ctcacaggcg acgcttattc catagtcatc gccaaaccgt tcacgtaaac gcttatccac
     1741 cagattcatc cccagcccac tggcattggt taccggttga tacaaaccgg cattgtcttc
     1801 gatctccagc atcaaatgtt gcccctcacg tcgggcgctg attgccactc gccctgtatc
     1861 cagcagttgt gatgtcccat gtttaatggc gttttccact atcggttgca gggtaaacgc
     1921 gggcaattgc tgctgggata attcttgcgg aatagcaatg ttgacctgca accgcgactg
     1981 gaagcgcgcc ttttcaattt gcagataagc gttcacatgt tcaatttcgt cggcgagagt
     2041 aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa gtggaaagat actgcaccag
     2101 ctggctggcc tgttcgctgt cgcggcggat caccgcttta atggtgttaa gcgcattaaa
     2161 caaaaaatga ggattcacct gggcgtgaag cagtttgatt tctgactggg tgagcatcgc
     2221 tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa agcagttgcg caatcccctc
     2281 gcccagcgtg cggtttattg aactgaataa acggtttttg gcttcataca atttgatggt
     2341 gcccatcacg cgctgatttt cgccacgcaa cggaattacc agcgtcgacc ccagtttgca
     2401 ttgcggatgc aaagagcaac gataaggtac ttcgttgcca tcagcgtaga ccacttcacc
     2461 ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt ttgccgggta aatggtggtc
     2521 gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg gtaatcgcga ctgcaccaat
     2581 atccagctcc tgatacagca cctgagccac tttcatgctg ttcacttcgt taaacccctg
     2641 tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca gtggcagaaa aagcagaagt
     2701 gtatttttca aacatcgcgc gtttatcgag caatatacgc ataaacagcg ccgcgccgac
     2761 ggtattggtg accatcattg gtgcagcaat attactcacc agacgcaccg catcttcata
     2821 aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt tcagcgacga acgtgacggc
     2881 accggcggta atggggttaa agactttatc agtgcgcccg cgacggatca ggatgctgtg
     2941 taccaggccg ccgagcaatc cttcaacgat ggttgagatc atgcaactta gcgcggtcat
     3001 gccccccatc gaatatcgat gtaagccgcc ggtcagacca accagcccac cgacgaccgg
     3061 accgccgagt aagccgccca ttaccgcgcc tatcgcacgg gtattggcaa tagaatcgtc
     3121 aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg gaaaagacga tgtagcagag
     3181 aaatttatgc ggcagacgaa ccgtgacctg cattaacggt atgaataatg gcgttttact
     3241 cattaaccac gcaatgacta aaaaaacgca catctgctga agcagcagca acaccagatt
     3301 aaaatcgtac atacccgcaa accacacttc cctttaaaac gcgtaacata cattgcctgc
     3361 gtttaacttt ctttgaactc ttgcagaaaa atgagaattc gtgagtacga tcactcaaaa
     3421 tcgcctggca aaaataaaat caccctatag atgcacaaaa aacgggcaaa actacctggt
     3481 tcgcaaaact gcgtctaaag ttaaaccggg acctcgcgag caagggtgag acgatggcgc
     3541 tttacacaat tggtgaagtg gcgttgcttt gtgatattaa ccctgtcacg ttacgcgcgt
     3601 ggcagaggcg ttacgggttg ctgaaaccgc aacggacaga cggcggtcat cgactgttca
     3661 acgatgccga tattgaccgt atccgcgaga tcaaacgctg gatcgacaac ggcgtgcagg
     3721 tcagcaaagt taaaatgctg ctcagtaatg aaaatgttga tgtgcagaac ggctggcgcg
     3781 atcagcaaga aacattactg acctacctgc aaagcggcaa tctgcatagc ctgcgaacgt
     3841 ggatcaaaga gcgcggtcag gattaccccg cccagacact caccacacat ctgtttattc
     3901 ctctgcgccg acgacttcaa tgccaacaac cgactctcca ggcgctgctg gcgatcctcg
     3961 acggcgtact gatcaactac atcgccattt gtctggcttc ggcacgtaaa aaacagggta
     4021 aagatgcgct ggtggttggc tggaatattc aggataccac ccgtctgtgg ctggagggct
     4081 ggattgccag tcaacaagga tggcgcattg atgtcctcgc ccactcgctc aatcaactac
     4141 gccctgaact attcgaaggt cgtacattgc tggtgtggtg cggtgaaaat cgaacctccg
     4201 cccaacagca gcaactcacc agttggcaag aacaaggcca tgatattttc ccactcggca
     4261 tttaatgatt cgttaacaaa tgcgctttac tgtacaatcc tttcgttaac ataaggagtg
     4321 cattatgcgc atagctaaaa ttggggtcat cgccctgttc ctgtttatgg cgttcggcgg
     4381 aattggtggc gtcatgctcg caggttatac ctttattttg cgtgctggct aagcgcctgc
     4441 accagccttt caaacaggcg gtctgcgatg atcgccgcca gtgccaccag taacgccccc
     4501 tggatcacat acgcggtatt aaatccgcta agcccgatga tgatgggcgt acccagcgtg
     4561 ctggccccta ccgttgaggc gatcgtcgcc gtaccaatgt tgataatcac cgaagttcgc
     4621 acgcccgcca gaatcaccgg agccgccagc ggtagctcga ccttacgcag tcgctgacca
     4681 cgactcattc ccataccttt cgcaacttct gtcacgctgg catcgatcgc tcccagcccg
     4741 gcaagtgtcg cctgcaggac gggcagcaca ccgtaaagga tcaaggcgat aatcgctggt
     4801 tgcagaccaa agccgatcac cggaacggca atcgccagca ctgcgacagg cggaaaagtc
     4861 tgtccaacgg cggcaatagt ttccaccagt gggcgaaatt ccgcgcccca cgggcgagtg
     4921 acagcaattc cggcaccagt gccaatgatc accgcaaaca aactcgaaat tcccaccagc
     4981 cagaaatgag ccagtgccag agctgcaaaa ctttcttgct gataaacggg tcgtggcagt
     5041 tgtgggaaca aggcagcaaa cagcggctgg ctgtaaggca gccagaaaat cagtgccaca
     5101 aacaaagcaa tgagccagaa cagcggatcg cgcaacatct tcatacgctt acgcctccac
     5161 cagcagatcc tgaaaatgca gcgtaccgca aggctggccc tgcgtgttca ccaccggcag
     5221 cacctcgcaa ccccgcgcga caaacaggga gagcgcatcg cgtagcgtca tctcttctgc
     5281 cagtgcctca ccttctgccc gttcttcgcg acgcacgtaa tccgccacac tacgtaacga
     5341 aagcagacgc acacccagtt cgctacgtcc aaaaaactgg cggacaaaat cattcgccgg
     5401 acgagtcagc atcgtcagcg gattgccctg ctgcaccact tcaccgtgat ccatcaatac
     5461 cagatgttca gccagccgta gcgcctcatc aatatcatga gtgaccagca caatagtgcg
     5521 ccccagcaaa cggtgaatgc gcgtcatctc ttgttgcaac gcgccgcgcg ttaccgggtc
     5581 cagtgcgcca aaaggttcat ccatcagtaa gacttgcgga tcggcagcca gtgcgcgcgc
     5641 cactcccaca cgttgctgct gaccaccgga aagctgatgc ggataacgct cacgcaaatt
     5701 tgactccagc cccagtagcg tcattaattc gtcgatacga tcgtcaatcc gtgcccgcga
     5761 ccatttttgt aattgcggca cggtagcgat gttttgcgcc acgctccagt gggggaatag
     5821 gccaatagat tgaatggcat agcccatccg gcggcgcaac tccagtactg gcagcgagcg
     5881 aatttcttct ccggcaaagc ggatctctcc gctgtcatgt tccaccaggc ggttaatcat
     5941 tttcaaggtg gtggatttgc cggagccaga tgtgccaatc agcaccgaaa aactcccttc
     6001 ctgaaaattg agattaagat cgttaacggc tttttgtgcg ccaaacagtt tgctgacatg
     6061 gctaaattca atcattacgt ttcaccttca gcagtgcgat aagcaaatcg aacagggcat
     6121 cgatcagcac cgccagaaca attaccggga tcacccccag caacactaaa tcaatggcgc
     6181 tgcttagcag cccctggaaa accagcgcac caaaaccgcc tgcgccgatt aacgccgcaa
     6241 tcaccgccat acctacagtt tgcaccatca ccacccgcag gctgcgcaga aataccggta
     6301 acgccagtgg taactgaaca tgcaggaatc gctgcgcccc gctcatgccc atcgctctgg
     6361 cgctctccag cacatcgcgc gggatctggt tcaaaccgac taccacgccg cgcaccagcg
     6421 gcagcaaggc atagagcacc agcgcaatca gtgcgggtgt cattccggtt cctgctatgc
     6481 cgagcttccc cagccacgga aaggccgtca ccagcgcggc aagcggcgca atcaacaggc
     6541 caaagagcgc caccgaaggc acggtctgaa tgacattgag cagagaaaaa attgccccct
     6601 gccgagcagt ggaaaagtag caccagatgc ccaacggcac accaatcact aacgcaggca
     6661 gcaccgcacc aaacagcaac gtcagatgtt gtgccagcgc gtcgtcaaac acatcctgac
     6721 ggttggcgta ttctttcatt agtgagagat cgttaagcgt gccggagtac agcaaccaca
     6781 gcggaataat ggcaatctgc atatgcaaca accagcgcca cagcggatgc gtggagattc
     6841 ggcggatggc atcgctacag gccagcaatg ccagcgccgc agccagccag aaaccactgc
     6901 cgaggctggt acgcgccagc gcactgccat tttgcgccag ttgtgtcgcc gcctttcccg
     6961 cgccccacac cagcaatacg aagacgaatt gcgccagaat gagtgcacaa atgctgcctt
     7021 ttttaccggg aataaaacag gccgtcagcc aggcgcaacc aacgcccacc agcatccaga
     7081 tcgtttgcgg ccacagttgc cagagatgac gcccttcacc cgagactaaa cgattaggcg
     7141 cgtagctgat aaacggcagc gctgctgcga ttgccgtcag caacagcagc agcgccagaa
     7201 caggattaat acgtagataa gtcacgggaa attacttcgc ccaccctttt tgtttcaggt
     7261 agtcggcagc cacttttttg gcatccagtc cttccactgc aatgctggca ttcagttgct
     7321 gcaatgtttt tgcatcgagg ctggcgaaga ctggctgtag ccactgtgcc atttgcggat
     7381 actccttcaa caccgattca cgcaccactg gtgcaggcgc atagataggt tgcacgcctt
     7441 gcggatcgct taaggtttgc agccccagcg ccgctaccga gccgtcagtg ccgtaagcca
     7501 ttgcagcatt aacgccagag gtttgctggg cagcggcttt gatcgtcacc gccgtgtcgc
     7561 caccagccag tgacagcaac tgatcctgac cgagcttaaa gccataggct ttttcaaacg
     7621 cgggtaacgc atcggcgcgt tcgataaact ctgccgaggc tgccagtttg aaggtgccgc
     7681 cctcttgcag ataacgactc aggtcggcaa gcgaagtgag tttatttttc tctgccacat
     7741 cctgacgcac ggcgatagtc caggtgttat ttgcaggcgc gggcgtcagc cagattaact
     7801 tgttgtgctc cgaatcgagc tttttgactt tctcgtaacc ttgctgcgcg tttttccacg
     7861 ctgcatcgtt ttcatcttta aagaagaaag cgccattgcc ggtatattcc ggatagatat
     7921 ccagttcacc ggaagtaatc gccccgcgca ccacaggagt cgttccgagt tgcactttat
     7981 tcaccgtcgg tacgccgtgg ctttccagca cctgcaaaat gatattgccg agtagcgcac
     8041 cttcggtatc gatttttgaa ccgactttaa cgggggaagc cgcctgtagc ggcaggctca
     8101 cggctgccaa cataaccagt gaacctgccc agagctttaa gagtggcatg atgctttcct
     8161 cattctttta ctgttgtttt cagcgaatta agagaaaagc atagttgata atggcggggt
     8221 tagcttgagc ggaaggattc agttgcagaa tcagataaat acttaagagg catattcggt
     8281 ccggcgtttc ctgccggatg cggcgcgagc gccttatccg gcctacaaag ggcgcaaacg
     8341 tcgcgccctt actaaagcat tacagcaact caaactcgcc tttcttaacg cgtgcggaat
     8401 cagtgccgat aaatacattg aacttgccag gctcggcgtc atatttcatc tgttgattcc
     8461 agaacttcag cgcctcaata tcgatcggga agctgacagt ctgagtttcg cccggtttca
     8521 gggtgatttt ctcaaagcct ttcagctgtt tcacagggcg actcatggaa gccgtcacat
     8581 cctgcaagta catctgcact accgtggccc cctcgcgctt accggtgttc gtcacctgca
     8641 cgctggcagt cactttgccg tcacgcttca tggtcggcgc agaaagtttc acatcagaga
     8701 cggtgaaagt ggtgtagctc agcccatagc cgaacggata caacgccccg ttagcttcat
     8761 caaaataacg cgaagtgtat ttgttcggct tgtcggcatt atacgggcga ccggtattca
     8821 gatggctgta gtacaccggg atctgcccga cagaacgcgg gaaggacatt ggcagcttgc
     8881 cggacgggtt gtaatcgcca aacaatacat cggcaattgc attaccgcct tcagtccccg
     8941 caaaccaggt ttccagaatc gcatcagcct gctgatcttc tttcaccagc gccagcggac
     9001 gcccgttcat cagcaccagc accagcggtt taccggtggc tttcagcgcc gcaatcaagt
     9061 cacgttggct ttgcggaata gtgatatcgg tccggctgga ggcttcgtgc gccatcccct
     9121 gtgcttcacc gactacagcc accaccacat cagattgttt cgccgtctgc accgcttcat
     9181 caatcatctc ttgcggcgaa cgcggatcga ctttgaccgc ttcttcatac tgattcagga
     9241 aatcgataat gcctttgtca ctggtaacgt tcgccccttt ggcatacagc actttaccgt
     9301 tttcaccgac ggaattttta atcccggtca gtacggtcac ggattgatcg gcaacaccgg
     9361 ctgcggacca gctgcccatc acgtcacgtt tactgtccgc cagtggccca accaccgcaa
     9421 tggtggccga ttttttcagc ggtaacgttt cgagacggtt tttcagcaac accaggcttt
     9481 cgcgcgccac ttcacgcgct tctttacggt gcaggcggct ttcggcattg gtatccaccg
     9541 ggtcagactc tttcggcccc aaatggctgt atgggtcgtt aaacaatccc atatcatatt
     9601 taacgttcag tacatggcgg gcagcatcgt ccagctcttc catcgtcact ttgccggatt
     9661 tgatcaaccc aggcagatac ttcgagtaat actcgtcgct catactcatg ttgattccgg
     9721 atttcagcgc cacgcgcacc gcatcttccg ggtctgccgc cgtgccatgt ttaatcagct
     9781 ctttgattgc accgtgatcg gaaacggtga tgcctttaaa gccccactgg tcgcgcagaa
     9841 catctttcag cagccaggaa tcggaggtgg ctggcgtgcc gttcagcgag ttcagcgcca
     9901 ccatcaccgc gccgctgcct gcgtccagcc ccgctttgta cggcggcata taatcattaa
     9961 acaggcgctg cggactcata tcgacggtgt tgtactcttt accgccttct accgcgccgt
    10021 atgcggcaaa gtgtttgacg ctggtcatca ccgagtagcg atctgccggg cttttaccct
    10081 gcatcgcttc caccatggtt ttacccattg ttgaggtgag atacgtatct tcgccaaaac
    10141 cttcggaagc acgtccccag cgcggatcgc gcgagacatc gaccatcggt gcccaggtca
    10201 tattcaggcc atcatctgcc gcttcataag cagagacacg tccgaccgtt ttcactgcat
    10261 cgaggttaaa agacgaggcc agaccgaggc taatcgggaa caccgtgcgc tgaccgtgca
    10321 gcacgtcgta agcaaagaaa agaggaattt tcaggcggct taattccatc acctgatcct
    10381 gcatggcgcg gatatcctga cgggttacgg tgttgaaaat cgccccaacc tgaccgtctt
    10441 tgatcatctc gcggatcgcc tctttcgggt tatccgggcc gacgctgatt aagcgcagct
    10501 gaccaatttt ctcatcaact gtcattttct taagcagttc ggtgacgaac gcatcccgcg
    10561 cttcgggcgt taatggatgg ttgccgaata aatcatccgc cagtgctggc tgcagggcca
    10621 gactcaccgc gattcctact gaacatagcc atttcatatg gatttttctt cctctgttgc
    10681 ccgacgaggc agcgaaaatg gtgcaaaaac cgtagtttgc cataagcatg atggagagag
    10741 aaaaagaatg ctcagtttat tgtctgaatt ttcaaaatat tcactcgctg aattgtcata
    10801 caaggcgcta ttctagtttg tgatattttt tcgccaccac aaggagtgga aaatgtcttc
    10861 catgacaaca actgataata aagccttttt gaatgaactt gcccgtctgg tcggtcattc
    10921 acacctgctc accgatcccg caaaaacggc ccgctatcgc aagggcttcc gttctggtca
    10981 gggcgacgcg ctggctgtcg ttttccctgg ctcactactg gaattgtggc gggtgctgaa
    11041 agcctgtgtc accgccgaca aaattattct gatgcaggct gccaatacag gcctgaccga
    11101 aggatcgacg ccaaacggta acgactatga tcgcgatatc gtgatcatca gcaccctgcg
    11161 tctcgacaag ctgcacgttc ttggcaaggg cgaacaggtg ctggcctatc cgggcaccac
    11221 gctctattcg ctggaaaaag ccctcaaacc gctgggacgc gaaccgcact cggtgattgg
    11281 atcgtcgtgt ataggcgcat cggttatcgg cggtatttgt aacaactccg gcggctcgct
    11341 ggtgcaacgt ggcccggcgt ataccgaaat gtcgttattc gcgcgtataa atgaagacgg
    11401 caaactgacg ctggtgaacc atctggggat tgatctgggc gaaacgccgg agcagatcct
    11461 tagcaagctg gatgatgatc gcatcaaaga tgacgatgtg cgtcacgatg gtcgtcacgc
    11521 ccacgattat gactatgtcc accgcgttcg tgatattgaa gccgacacgc ccgcacgtta
    11581 taacgccgat ccggatcggt tatttgaatc ttctggttgc gccgggaagc tggccgtctt
    11641 tgcggtacgt ctagatacct tcgaagcgga aaaaaatcag caggtgtttt atatcggcac
    11701 caaccagccg gaagtgctga ccgaaatccg ccgtcatatt ctggctaact tcgaaaatct
    11761 gccggttgcc ggggaatata tgcaccggga tatctacgat attgcggaaa aatacggcaa
    11821 agacaccttc ctgatgattg ataagttagg caccgacaag atgccgttct tctttaatct
    11881 caagggacgc accgatgcga tgctggagaa agtgaaattc ttccgtccgc attttactga
    11941 ccgtgcgatg caaaaattcg gtcacctgtt ccccagccat ttaccgccgc gcatgaaaaa
    12001 ctggcgcgat aaatacgagc atcatctgct gttaaaaatg gcgggcgatg gcgtgggcga
    12061 agccaaatcg tggttggtgg attatttcaa acaggccgaa ggcgatttct ttgtctgtac
    12121 gccggaggaa ggcagcaaag cgtttttaca ccgtttcgcc gctgcgggcg cagcaattcg
    12181 ttatcaggcg gtgcattccg atgaagtcga agacattctg gcgttggata tcgctctgcg
    12241 gcgtaacgac accgagtggt atgagcattt accgccggag atcgacagcc agctggtgca
    12301 caagctctat tacggccatt ttatgtgcta tgtcttccat caggattaca tagtgaaaaa
    12361 aggcgtggat gtgcatgcgt taaaagaaca gatgctggaa ctgctacagc agcgcggcgc
    12421 gcaataccct gccgagcata acgtcggtca tttgtataaa gcaccggaga cgttgcagaa
    12481 gttctatcgc gagaacgatc cgaccaacag catgaatccg gggatcggta aaaccagtaa
    12541 acggaaaaac tggcaggaag tggagtaaaa attacggatg gcagagtatc gccatccgaa
    12601 ttcacttaat cgttctgtgc cgtctgcccc gccgccgcca tttgggcggc tttttgtttt
    12661 ttatagctca acgctgctgc cggcacaggc atcactttac cggtttcaat ccaggtacgc
    12721 aggcggctgg catcggcaaa atgggtatat ttgccaaacg cgtccatcac taccagcgcc
    12781 accggtttat tattgataac cgtacgcatc accagacaat ggcccgccgc attggtaaag
    12841 ccggttttgg ttaactgaat attccagtta tcgcgataca ccagatgatt agtattgcgg
    12901 aacggcagcg tatacgtcgg gttagagaag gttgccatat cttcccgggt agtacttaac
    12961 tgcccgatca acggatactg tttgctggca atgagcagtt tggttaagtc acgggcagtt
    13021 gaaacgttat gcaccgacaa tccggtaggt tcaacaaagc gcgtgttgtt cattccgagc
    13081 gatttcgctt tcgcattcat tgccttaata aaggctttgt aaccaccggg ataatggtgc
    13141 gcaaggcttg ccgccgcgcg gttttctgaa gacatcagcg ccagcaacag catatcttta
    13201 cggctgattt cgctattcag tcgtacgcgc gaatagaccc ctttcatctc cggcgtctgg
    13261 ctgatatcca cttttagttt ttcatccagc ggcagtcgtg catccagcac aaccatcgcg
    13321 gtcattaatt tgctgataga cgcaatcgga cgcaccagat ccgggtggtt cgaatagatc
    13381 actttgttgg tattcagatc aacaatcatc gcgctaccgg aggcaatttc cggttgtgaa
    13441 gcggtggtag cggctgccgt tttcgcaacg gcctgcggtg ccaaaggcac agccagcatc
    13501 agggccaggc taaataaaga aactcgaaat ttcggcatga tgagcattca gatagtggtt
    13561 cacgcgcacg ggttgcgcac cgccggagta aggatttact gaggctagcg acgccatcat
    13621 aacgagcaaa aagtgcgatc gtcaaaggag aatcgtgagg aaatgctgca ttgctgacat
    13681 ttacgccagc aatgcaatgt caaagaactt tctagaacag gcgatacccg tagccccata
    13741 gtataacggt tagggcgagc agcacttcca gtaccagcac gccaatcgcc agcgtcgaac
    13801 tggagaagct aaggccttcc tctttgttga tattcaggaa gctcggaata ccaaggtaaa
    13861 gcaggtagcc ggtgtaaaac agcgccaccg tgccgaccag cgcacacaac cagaccagcg
    13921 gataaagcgc caccagaccg cttaaaaaca gcggagttgc aacgtagccc gcgaagacca
    13981 tgcagtgcgc aagtgacgga cgctgcgggt aattgcgcgc catccaccag atgacccgcc
    14041 ccatcaccgc aaccccagcg agcataacgc cataaaacag gacagccagc gccagtccgg
    14101 taaaccagga caacttcagg atagtgccat cgccaaaatt ccagccaatc tgtgtagtgc
    14161 caatgaaggc gcaaatcacc ggaatcgccg ccatcagcaa aacgtggtgg gtgtaatggt
    14221 gagaaatcgt ttcgttttcg cgattaatca cctgcatttc acgatcggga tgggaaaaca
    14281 gtccccagac atggctcata ccgccccctt gttgtgagtt catgaacctg acagttcaag
    14341 tataagtcag cttgtgatta ttttttgttc gctcatgtaa tttgcaccgt ctgatgtacg
    14401 tcctttttca gcgtgaatga tttccagggt gtatgcttac aggcaaccaa agggagacag
    14461 actggcctat ggatctcaat acactcatct cacaatatgg ttatgccgcg ctggtgatcg
    14521 gtagcctggc ggaaggtgaa accgtgactt tgctgggagg cgttgcggcg catcaggggc
    14581 tattaaagtt cccgctggtg gtactttctg tggcgcttgg cggcatgatt ggcgaccagg
    14641 tgctttatct gtgcgggcgg cggtttggcg gcaagctgtt acgccgtttc tcgaaacatc
    14701 aggataaaat tgagcgggcg cagaaactta tccaacgcca tccgtatctg tttgtcattg
    14761 gtacgcgctt tatgtatggc tttcgggtga ttggcccgac gctgattggt gccagccagc
    14821 tgccgccgaa aatctttctg ccgcttaata ttctcggcgc atttgcctgg gcgttgattt
    14881 ttaccactat cggttacgct ggtggtcagg tgattgcgcc gtggttgcac aatctcgatc
    14941 agcatttgaa gcactgggtc tggttgattc tggtcgtggt tctggtggta ggcgtgcgct
    15001 ggtggctgaa acgacgcggg aagaaaaagc cggataatca ggcgtaaaac cattgccctg
    15061 gataaggcgt tcgcgccgca tccgacatcc ggtgcaccat gcctgatgcg gcgcttgtgc
    15121 gtcttatcat gcctaccaaa attttaccca ttgaaatatc cacacacctc actgcgctat
    15181 tctgggttga actgcggatt cgccaacata aagccgccat ccactatcaa cgactgcccg
    15241 gtggtgtaat ttgcgccctc cgaacaaagc cacgccacca ggctggcaat ctcatacgtt
    15301 gcgccaaaac gccgcagagg aatcgaaggc tccgcgtcgg gcttcacgtc gctatcatcc
    15361 atgccgttca ttggcgtagc gatcgcccca ggtgcgactg cgttcaccaa aatcttatgc
    15421 ctgaccaact ccagcgccat tgctttggtt aacccgccga gcgcatgttt agcggctgtg
    15481 taggcgctgg catccggcag cggcgtatgt tcatgtaccg acgtaatgtt gatgatgcga
    15541 ccaccctgcc cttgtttcac catctggcgg gccgcaattt gtgagcataa aaatgcacca
    15601 tcgacatcaa cggtaaaaat cttgcgccac tcatcgaaag ccatatcaag aaacggcgct
    15661 ttggtcattg cgcccgcatt attcaccagc acatcaatac gccccagccg ttgaatgagt
    15721 ttctccagcg cctgtgcacc ttctggcagt ttaccgagat ccagctgcac aatctccgca
    15781 cgtacgccgt ggctaactac ctcacgcgcg gtatcttttg ccccttcttc atctgagtgc
    15841 caggtaatgc caatatcaaa cccctgctgc gccagtaata acgcgcactc tttgccgatc
    15901 cccgaatcgg aggcggtaat aatcgcaacc tgtgccatcg agttctccac ttaacgctga
    15961 ataaacgtta agtatagaag gcgcatatca tcagcgtttg taacccccgc ccaacgcacc
    16021 agtgagttga atggaagcat ccagccactg cccttgcaat aacaggccat tggcccgctc
    16081 acgcagcgcg gggattctgg cttcgctgac gcgggaacca gcaatgatgc ccgcgttaaa
    16141 gcgcgcctgc gcaagaccta ccacacgcaa ggcatcgcgc tcaatttgcg cctgatgctg
    16201 gtttttctcc gcaagtgtct gaacctgact ggctgcccgc gccacgtcat tcaccgcttc
    16261 aaccaccgct ttgttgtagc tggcgataga caagttgctt tcggcttttg cgatatcgag
    16321 attggcgtta agacgaccac tatcgaaaat gggtagcgtc aggcctgccg taacgcccat
    16381 ttgctgcgcg gaatgacgga acagatcgct taagtgcaac gcatcctgtt gcaggaaggc
    16441 catcaggttg atgtcaggat aaaatgccgc ttttgccgca tcaatggtgc ttagcgatga
    16501 ctcaacgtac cagtgcgccg cctgcacatc tgcccgccgg gccagtaagg agtaccccag
    16561 ttcatcagga agctggcttg ccactttcgg caacgcgacc ggtttaagct tcaatgactt
    16621 tgtctggtta tttgtaagtg cgcttaaccg tgcctcaata attttcattt tccccgcgac
    16681 atcgttgagc tgctgccggg ttttgctggc attaatatcg gtttccacac cttcaactga
    16741 agaagtaatc ccgttctgat atagctggcg atcggtcgcg ataatggtgt tctgctcttt
    16801 ttctatttgc tgcaagaccg tgtttaacgc cgcctgggtt tgccactccc agtacaggcg
    16861 ggctacgctg ccagccagca attggcgggt ttgctcgcgt tccgccgccc gtgctttaac
    16921 cgtacccagg cgggcagtaa cctccgcccg attctttccc cagatatcga gatgccagcc
    16981 cgccgttaag ccaaaagttc cgttggtgta ccacggaccg gtcgtacctg cggccggatc
    17041 gttcagagca aacggcccca ttaagccttc tgccgacatt ttttgccgct ccatatccgc
    17101 agaaaagtcg atctgcggac catcctgagt agcaattgcc ttcgcctggg cttcagctag
    17161 ctgaatgcgc tgttcagcca cctgcatatc cggtgcgttc tgtagtgcat tgttaattaa
    17221 ggaagtgagt tgattatcgt gatactccag ccaccattgg ctgtctggcc aaccattttt
    17281 cagcgccgtg ggtaatgcgg tgtcaacttg tgcagcgggc gtttgctggc ttaacgcctg
    17341 gcgggtttca tgcataggcg cacacccggc cagcatcagt aacagcggaa aacaggcgat
    17401 ggcgggataa aaggaatcac gattcatggg ggaataatca ggtaagaaaa ggtgcgcgga
    17461 gattaccgtg tgttgcgata tattttttag tttcgcgtgg caatacatca gtagcaataa
    17521 aacgacatat ccagaaaaat atacactaag tgaatgatat cttccgattt atcttaatcg
    17581 tttatggata acggcaaagg gcttcgtttt ttcctatact tattcagcac tcacaaataa
    17641 aggaacgcca atgaaaatta tactctgggc tgtattgatt attttcctga ttgggctact
    17701 ggtggtgact ggcgtattta agatgatatt ttaaaaatca gatatggcat ataagttgtc
    17761 aggtcaggaa ctagatattt tgtgtacttg atttgcaaac agctccggtg ttggtggcgt
    17821 caaaatcgct gaagcgttcc ccgaaggccg gggcagccca catggatgtg ggctgagggc
    17881 gcgtcttaca aggacgttac ctcgcgcccg acccggtaga cgaaggggat aagctgaagg
    17941 taccgcgcag cggcgatttt gttcgccaga gcccgggggt gcagggggag gaggcgattg
    18001 tcctccccct gcgcgctcct tgcgccagtg gcaatatgtg gcttagctca tgaaaggagc
    18061 gcaacaagat gatgaagctg aataagcgat tcattgtctc tgataaactg cgattccact
    18121 attatcgcag cttatcgata tcaattgcct gaatagccct tgcaatatca ggggaattat
    18181 tcaacacccg aacatgctga aataattccg ttgcttcatc gtattcttta cgcaaataac
    18241 tcaaccactg tttaatccgc gcaacgtgat ataacccggt atcgccctgc ttttccagac
    18301 gggtatattt ttgcagcaaa gcaaccacct ccggccacgg cattcgcggt tcgttatatt
    18361 ttactacccg gctcaggttg ggaatattga gcgccccgcg accaatcatc actgcgtcgc
    18421 agccgctgat cgcaatgcat tgttgcgcgc tctgccagtc ccagatttcg ccgttggcaa
    18481 tcaccggaat attcagccgc tggcgaatct cgccaatcgc ctgccagtca atatgctccg
    18541 cgcggtaacc ctgctctttt gtccgtccat gcaccaccag ctccgtagca ccagcctgtt
    18601 gaaccgcatc ggcgatttca aatttcttct caccgctgtc ccagcccaga cgcactttca
    18661 cgctgacggg caaatgcgcc ggtacagctt cacgcatcgc ttttgcaccc tggtagatga
    18721 gttcaggatc tttgagtaac gtcgccccac caccgctacc gttgaccgtt ttcgacgggc
    18781 agccgcaatt gagatccacg ccccaggaac ctaattccac cgcacgggcg gcgttctctg
    18841 ccagccattg tgggaactga cccaacaact gcacgcgcac cagcgtaccg gatggtgtcc
    18901 ggctggcgtt ttgtagctca gggcaaatgc gatgaaaaac ttttaccggc agcagttgat
    18961 ccaccacgcg gacaaactcg gtgatgcaca gatcgtagtc gttaacttcg gtcagcaatt
    19021 cacgcaccag agagtcaagc acaccctcca tcggagccag taacacacgc atatcttcac
    19081 ccgcaaaaaa atgaggcgct atgttagcgc ctatggtcaa cggattaaag gtctgcaaag
    19141 agggattgct gcaaactgta atccctctct atgcttgatg tatgcctgag ttcgttgcgc
    19201 attgcgtaat gccggggagc gaaaaaataa gcgctgattt cttaatgtga tcggtagcac
    19261 gttttacgaa ttaattgtat gatgaatcca tctcatctgg ggtgttgatt atgagtaaga
    19321 cactgaacat tatctggcaa tatttacgcg ctttcgtcct gatttatgcc tgcctgtatg
    19381 caggcatttt cattgcttcc ctgctaccgg taaccattcc gggcagcatc atcggtatgc
    19441 tgatcctgtt tgtcctgctg gccttgcaaa ttcttccggc aaaatgggtc aatccggggt
    19501 gctacgtact gattcgctat atggcgctat tgtttgtgcc gattggcgta ggcgtcatgc
    19561 aatattttga tttgctccgc gcacagtttg gcccggtagt ggtttcctgt gcaatcagta
    19621 cgctggtggt ttttctggtg gtgagctgga gttcgcaact ggtacacggt gaacgtaaag
    19681 tcgtaggtca gaaaggatca gaagaatgat ggcaaatatc tggtggtcat taccgctgac
    19741 gttgattgtc ttttttgccg cccgcaaact ggcggcacgg tataagtttc ccctgcttaa
    19801 cccgttgctg gtagcaatgg tggtgatcat tcctttttta atgctgaccg gcatctctta
    19861 cgacagctac tttaaaggta gcgaagtgct aaacgatctg ctgcaaccgg cggtcgtcgc
    19921 gctggcctat cctttatatg agcagctaca ccagatccgc gcgcgctgga aatcgatcat
    19981 caccatctgt tttatcggca gcgtggttgc gatggtgacg gggacttccg tggcattgtt
    20041 gatgggcgct tcaccggaaa tcgccgcgtc aatcctgccg aaatcagtca ccacgcctat
    20101 tgcaatggcg gttggcggca gtattggcgg tattccggca atcagcgcag tttgcgtgat
    20161 tttcgtcggc atcctcggcg cggtatttgg ccatacattg cttaatgcga tgcgtattcg
    20221 taccaaagct gcgcgcggtc tggcaatggg gactgcctcg cacgccctcg gtacggcgcg
    20281 ctgcgccgag ctggattatc aggaaggtgc atttagttcg ctagcgctgg tgttatgcgg
    20341 gataattact tcgctgatcg caccgttcct tttcccgatt attctggcag taatgggcta
    20401 aaatttgcga tgcgtcgcgc atttttgatg taagtttcac gcgttgcata attaatgaga
    20461 ttcagatcac atataaagcc ataacgggtt cgtaaactgt tatcccatta catgattatg
    20521 aggcaacgcc atgcatccac gttttcaaac cgcttttgcc caacttgcgg ataacttgca
    20581 atctgcactg gaacctattc tggcagacaa gtacttcccc gctttgttga ccggggagca
    20641 agtctcatcg ctgaagagcg caacggggct ggacgaagac gcgctggcat tcgcactact
    20701 tccgctggcg gcggcctgtg cgcgtacgcc attgtcgaat tttaatgttg gcgcaattgc
    20761 gcgcggtgtg agcggaacct ggtatttcgg tgccaatatg gaatttattg gtgcgacaat
    20821 gcagcaaacc gttcatgccg aacaaagcgc gatcagccac gcctggttga gtggtgaaaa
    20881 agcgcttgca gccatcaccg ttaactacac gccttgtggt cactgccgtc agtttatgaa
    20941 tgaactgaac agcggtctgg atctgcgtat tcatctgccg ggccgcgagg cacacgcgct
    21001 gcgtgactat ctgccggatg cctttgggcc gaaagatctg gagattaaaa cgctgctgat
    21061 ggacgagcag gatcacggct atgcgctgac tggtgatgcg ctttctcagg cggcgattgc
    21121 agcggcaaac cgttcgcaca tgccttacag taagtcgcca agcggtgtcg cgctggaatg
    21181 taaagacggt cgtattttca gtggcagcta cgctgaaaac gccgcattca acccgactct
    21241 gccaccgttg cagggagcgt taattctgtt gaatctcaag ggttatgatt acccggatat
    21301 ccagcgcgcg gttctggcag aaaaagccga tgcgccgttg attcagtggg atgccacctc
    21361 cgcaacgctg aaagctctcg gctgtcacag tatcgaccga gtgcttctcg cttaagcctg
    21421 gtgccggatg cggcgtgaac gccttgtccg gcttgccagc cctctcctgg tgtcgaaatt
    21481 cccggcaaac agtttgccgt ttcttgcgca aaaccagcgg gtaaagtagc ctgatggaaa
    21541 ttttccttag atcgagtctc ctgcatgtta aagcgcgtgt tcctcagcct gttagtcctg
    21601 atcggcttgc tgctgttgac tgtgctcggc ctcgatcgct ggatgagctg gaaaaccgcg
    21661 ccttatatct acgacgaatt gcaggatctc ccctaccgcc aggtcggtgt ggtgctcgga
    21721 acagcaaaat attatcgtac tggcgtaatt aatcagtatt atcgctaccg cattcaagga
    21781 gcgattaatg cctataacag cggtaaggta aattatctat tactgagcgg cgataacgca
    21841 ttgcaaagtt ataatgagcc gatgaccatg cgcaaagatt taatcgctgc tggtgtcgac
    21901 ccatcagata ttgttctcga ttacgcaggc tttcgtacgc tggattccat cgtgcgtaca
    21961 cgcaaagttt tcgatactaa tgatttcatt attatcaccc aacgtttcca ctgtgagcgg
    22021 gcattattta ttgcgctgca tatggggatt caggctcagt gttatgccgt accgtcaccg
    22081 aaagatatgc tgtcagtacg tattcgcgaa tttgccgccc gtttcggtgc gctggctgac
    22141 ctttatattt ttaaacgtga accgcgtttt ttagggccgc tggtccctat tccggctatg
    22201 caccaggtgc cggaagatgc gcaggggtat cccgccgtca cacccgaaca gttacttgaa
    22261 ttacaaaaga aacaaggaaa gtagttatgg atgtacagca gttttttgtc gttgccgttt
    22321 ttttccttat tccgatattt tgtttccgcg aagcatggaa aggctggcgc gcaggcgcga
    22381 ttgataaacg ggttaaaaat gcaccggaac cggtgtatgt ctggcgagca aaaaatcccg
    22441 gactcttttt cgcttatatg gtggcatata tcggcttcgg aattttatct atcggcatga
    22501 ttgtttatct tattttctat cgttaatatt ttctcttttc tgttaattat tgagaataat
    22561 tattacttca ctcaataagc tgcggatata acattctgaa ccgcagctat ttgtgaatcc
    22621 tttcacagta taaatttccc cacgcgcata gccttaatat cagcacgcaa tatttatact
    22681 ctaaataatt cgagttgcag gaaggcgaca agtgagtgag tccccaggag cttacataag
    22741 taagtgactg gggtgagcga acgcagacgc agcacatgca acttgaagta tgacgagtat
    22801 atttagcgtt cgccttaatt actcatagca ttaaggaaga tcacatgccg caacaaaatt
    22861 atctggatga actcactccg gcttttacgc ctttactggc gattaaagaa gcctctcgct
    22921 gtttattatg tcatgacgct ccctgtagtc aggcttgccc ggcacagacc gatccgggga
    22981 aatttattcg ctcaatctac tttcgtaatt ttaaaggcgc tgccgagaca attcgcgaaa
    23041 ataatgccct cggtgccgtt tgtgccagag tgtgcccgac ggagaaatta tgtcaaagcg
    23101 gttgtacccg tgccggtgtt gatactccca ttgatatcgg tcgcttacag cgttttgtta
    23161 ctgattttga acaacaaacc gggatggaaa tttatcagcc cggtactaaa acgctcggca
    23221 aagtcgcgat tattggcgca ggtcctgccg gattgcaggc cagtgtaaca ctgacaaacc
    23281 agggttatga cgtcacgatt tatgagaaag aagcgcaccc cggtggctgg ttgcgtaacg
    23341 gtattccgca attccggtta ccgcaatcag tgctggatgc agagatcgcc cgtattgaaa
    23401 aaatgggcgt gaccattaag tgcaacaacg aagtcggtaa cacactcacc cttgagcagc
    23461 tgaaagcaga aaaccgcgcg gtactggtca ccgtggggtt atcgagcggt tccgggttat
    23521 cgctgtttga gcatagcgac gttgagattg ccgtcgactt cctgcaacgt gcacgacagg
    23581 cgcaaggcga tatcagcatt ccacaaagcg cattaattat cggcggcggt gatgttgcga
    23641 tggacgtagc cagcacgctg aaagttctcg gctgtcaggc ggtaacctgc gtagcgcgtg
    23701 aagagttaga tgagtttccg gcaagcgaaa aagagtttgc cagcgcccgg gagctgggcg
    23761 tttcgatcat cgatggattc acgccagtag ccgtggaagg caataaagtc acgtttaagc
    23821 atgtacggtt accgggcgaa ctgacgatgg cggcagataa aattattctc gccgtcggtc
    23881 agcatgccag actggatgcc tttgcggagt tagagccgca gcgtaacacc atcaaaacac
    23941 aaaattatca gacccgcgat ccgcaagtct ttgctgctgg cgatattgtt gagggtgaca
    24001 aaaccgtggt ctatgccgtg aaaaccggga aagaagccgc cgaggcgatt catcactatt
    24061 tagagggagc ttgctcatgt taacgaaaga tctttcgatt actttttgcg gcgtgaagtt
    24121 tcccaacccg ttctgcctct cttcttcgcc tgtaggcaac tgctatgaga tgtgtgccaa
    24181 agcctacgac acaggttggg gcggcgtggt gtttaaaacg atcggctttt ttatcgccaa
    24241 cgaagtctcg ccgcgttttg atcatctggt gaaagaagat accggtttta tcggcttcaa
    24301 aaatatggag cagattgctg aacatccgtt ggaagagaat ctggccgccc tgcgtcagct
    24361 gaaggaagat tacccggaca aagtattgat cgcttcgatc atgggggaaa atgagcagca
    24421 atgggaggag ctggcgcgcc tggtgcaaga agctggcgcg gatatgatcg agtgtaactt
    24481 ctcctgtccg caaatgactt ctcatgcgat gggtagcgat gtcgggcaaa gcccggagct
    24541 ggtagaaaaa tattgtcggg cagtgaaacg gggttccacg ctgccaatgc tggcgaagat
    24601 gacgccgaat atcggtgata tgtgcgaagt ggcgctggcg gcgaagcgcg gcggcgcaga
    24661 tggcattgcg gcgattaaca ccgttaaatc catcaccaat atcgatctta atcagaaaat
    24721 cggtatgccg atcgttaacg gaaaatcgag tatttccgga tattccggta aagcggtaaa
    24781 accgatcgcc ctgcgcttca ttcagcagat gcgcacccat ccagaactgc gcgatttccc
    24841 aatcagcggt atcggcggca ttgaaacctg ggaggatgcg gctgagtttt tattgctcgg
    24901 cgcagcaacg ttacaggtga ccaccggcat catgcagtac gggtatcgga tagtggaaga
    24961 tatggcaagc gggttgtcgc attatctcgc cgatcaggga tttgattcgc tgcaggagat
    25021 ggtaggtctg gcgaataaca atattgtccc ggcggaagat ttagaccgca gttatattgt
    25081 ctatccccgt atcaatcttg ataaatgtgt tggctgtgga cgctgttata tttcctgtta
    25141 cgacggcggt caccaggcga tggaatggag cgagaaaacc cgcacaccgc attgtaatac
    25201 cgagaaatgt gtgggttgtc tgctttgtgg tcacgtctgc ccggtgggtt gtattgagct
    25261 cggggaagtg aagtttaaga aaggcgagaa agaacacccg gtaacgttgt aaaaccagag
    25321 acgcatccgg catttggcac cgattgccgg atgcagcgca tccggcaata tccacaatca
    25381 catcacttct tacgcgcgta tttcagtgaa tccagcgcta cggcgaagat aataatcgcc
    25441 cctttgatga tgtactgcca gtatgggttt acgccgatat acgtcaggcc atagttgatg
    25501 acggtaaaaa taattacccc ggtcaccacg ccaatcaccg tccccacacc gccgctgaac
    25561 gatacaccgc ctaccacgca cgccgcgata gcatccagct catacataaa gccgaggttg
    25621 ttggtggcag agccgatacg tccggcttct aacatcccgc caaaggcata gaacacgcca
    25681 gacaacgcgt agatcatcag caggttcagg ccgacgttga caccagatac ttttgccgct
    25741 tccgggttac cgccaatggc aaaaatgttc ttaccgaagc gggttttgtt ccacaacacc
    25801 cagacgaacg ccaccgcaat caacgcgtag aaggtgatgt aagagagacg gaaactcccc
    25861 agcgcgacaa agccctgagc aaaggtagag aagccactgt caaaaccaga aattggcgac
    25921 gccccgacaa agtcgtaata gagcgagttg attccataga cgatgatcat cgtgccgagc
    25981 gtggtaatga acggcgtcac gttgagataa gcgataatca gaccgttgat caaaccgatc
    26041 accgcaccaa tggcacagac aatcagaata accagcgcaa tcggcatcgt cgccatttcc
    26101 gggaacactt tgttggcgtt atccatggac tgcaataatg tcgcagccac cactgccgcc
    26161 agccctacct gacgaccagc agaaagatcg gtcccctggg tgacaattaa ccctgccaca
    26221 ccgagcgcga taataatacg caccgatgac tgggtgagaa tattacttaa gttcaacaga
    26281 cttaaaaatg ttgggtcctg gaaaataata atcgccagca aaactaaaag aacgacgtaa
    26341 ataccgccct ctttcaggta agtaagaaaa cttttcttat ttaacgcact catgggaagc
    26401 ccctaatctt aaaggtgcaa agacgcaaga cgcagaattt cgttttgcgt tgttgttttt
    26461 gtatcgacaa ttccggaaac gagaccattg ctcatgacca gaatacggtc tgttatccct
    26521 aacaactcag gcatttcaga ggagataata ataatcccct tgcctttctt cgccagttcg
    26581 gcaattaact gataaatttc aaacttcgcc ccgacatcaa taccgcgcgt cggttcatcg
    26641 agcattaata tttctggttg cgttagtagc cagcgaccaa taatcacctt ttgctgatta
    26701 ccaccggaga gcgaaccaat ttgcgtccga tgacccggcg tttttacccg catcgaatca
    26761 atcacccact gggtatcgct tttcatccgc gagttatcca gtaaaccaac tttatttttg
    26821 tagttgcgaa tattggaaat taacgagtta aaactaatat ccagataggc ataaattccc
    26881 gttgagcggc gctcctcagt taccagtgca aatccgtggt ttatggcttc gttggcatta
    26941 tggttattga tctgtttgcc gtgcaacgta atggtgccag ccgatttctc gcgaatacca
    27001 aataacgtct caacaatatc ggtacgtttc gcccccacca gcccggcaat accgaggatc
    27061 tcccctttat gcagatcaaa cgagacatcg cgaatcgacg gctggcgcag tgacgttagg
    27121 ttacgcacct cgaggatgac ttcgcccggc ttgttttctt tgtcaggaaa acgctgatta
    27181 agagaacgcc caaccatcat ggcgatgatc ttgtccatcg tcagtcctgc cagcggttcg
    27241 gtggcgatcc actgaccgtc gcgcaatacg gtaacttcat cacataactg gaagatttct
    27301 tccattttat gcgagatata aacaataccg cagccgcgct cttttaattt acgaataata
    27361 gtgaacagat gattgacctc tttttcggtt aacgaagaag tcggttcatc cataatcaca
    27421 attttcgcgt tataggaaaa cgctttggcg atttcgatca tctgcatttg cgaaacggat
    27481 aatgtgccga cgcgcgcacg cggatcgata tcaatatcca gttcatcaaa aatcgctttg
    27541 gtttcgcggt acattttgtc ctgatcgaca aacatgcctt tggtgggata tcgccccagc
    27601 cacatgttat ccatcaccga acgttgtaat accaggttta actcctggtg taccatcgaa
    27661 ataccatttt ccagtgcctc tttggcggaa tggaaatcga tctctttacc ctggaataaa
    27721 atggtgccgg agtctttttg ataaatacca aacaggcatt ttaataatgt cgattttcct
    27781 gcaccgtttt cccccattaa tgcatggata gaatgtggcc ggacttttaa attaacgtta
    27841 tcaagtgcct taacaccagg aaaggacttg ttgataccgc tcatttccaa caagtattcc
    27901 ccggaagacg gagtcgttga gctgaccata taattttacc ttgttggcca tacaataagg
    27961 gcgcagtaaa cgactgcgcc caatcagtct tatttcttgc tgaattcagc caggttgtct
    28021 ttatctacgc caacataagg tacgcggacc actttgttgt cgattttcca gttggtgcca
    28081 tcagccgcac ctttgccatc ggccaggttt ttcgccagat caaaggtcgc tttcgcctgg
    28141 ttgttagcat cgttcagtac tgtgcccgcc agtgcaccgg atttcaccag cgccagcgct
    28201 tctggcagcg catcgacgcc aaacaccgga atgctggact tgttgtgtgc tttcagtgct
    28261 tctaccgcgc ccattgccat cgcatcgttg ttggcgataa ccacttcgat tttgttggcg
    28321 ttcgggccag acagccaggc gtccatctta tctttcgcct gagcggtatc ccacatagcg
    28381 gtatctaact gtaactgttc agttttaatg cctttgtcgt tcagctcttt aatcacgtaa
    28441 gtggtacgtg cttctgcatc cggatggccc ggttcacctt tcagcaatac gaattgaatc
    28501 tgaccgtctt tgttcagatc ccaaccctga ttcgccgccc agtgtttagc aatcaaatcg
    28561 ccctgaataa tgccggactc tttggaatca gtgccaacgt agtaggcttt gtcgtagcta
    28621 tccagcgcct tacgagacgg ttctttgttg aagaaaacca ccggcacgtt ttgcccacgc
    28681 gctttctcaa tcaccgtacc cgcagctgcc gggtcaacca ggttgattgc cagtgccttc
    28741 acccctttcg ccagcaatac gtcgatctga tcgttctgct tggactggtc attctgagaa
    28801 tcattcatca gcagctgaac atctggcgcg gctttcgcat cttgctcaat agccttgcgc
    28861 actacagaca taaagttatc gtcgtactta tagattgtta caccaatgcg agtatcagca
    28921 gcgtgtgcag cggcaccgaa taacatgctg gccatcacag cagacagggt taacaccttc
    28981 ttattcatgg tatctccggt ttttcttatg cagggtagtg cttgagataa atgctcggcg
    29041 gggcagtaga gttaatgaag tgttactgaa cgccgaagct cactttttaa aattcgttct
    29101 tccatgcccg gtaacgctcc agaaaacggc tttaattgtt gttatgacgc tattacctcg
    29161 gtgaaagtga ttattcactg ttacatacgg gttacaacgt taaaacggtg caatcatagc
    29221 tatcacattg ttaagatact gtgaaatcac tcacagattg aaagcggtta catcgcctga
    29281 tttgttgagt tagtgatcgt cgccgcattc tggcgtgttg cgacagaatg gcgacgcact
    29341 aacgtcggca taaaacagtg gctagcacga ggatcaatat tgcctgctgc cccctgcaag
    29401 gccagttcgg tggctaattt cgccattgaa gcaatgggat aacgcacggt cgttaattgc
    29461 gggtcggtgt aacgggcgat gggaatatca tcgaaaccga tgattgagag atgtaacgga
    29521 atcgcaatgc cattatcttt taatgctgtc agtgcgccag cagccatatt gtcgttataa
    29581 gcaaatacag cggtaagttg tagattgcgc cccagcagtt caaccatcgc cgcctcaccg
    29641 cccggcatgt ccggcgtacc agtgccaatc cagctttccg gcggaataat atcctgctct
    29701 ttcaacgcac tcatccagcc tgctttacgc atggcgtcat cttcaatgcc gtgactggaa
    29761 gaaagataac caatacgttg atgaccgtta ttcagcaaca tgcgcgtcgc cattcgggca
    29821 ccgctgagat tatccaggca aacgcaacga tgggcgtacc ccggcacaac gcggttgatt
    29881 aacaccatac cgggaatgtt atccataaat tgcgccagtt catcgtcact caatgctttt
    29941 gagtgaacaa tcaacgcatt acaacgctgg cgaattaaca cctcaatggc gtgacgctct
    30001 ttttccgctt catgatagct attgccgatt agcacgtatt tctgatgctg ctgagcgacc
    30061 agatccaccg cttttaccag cgcgccgaaa aacgcatcag aaacgtccat caccaccacg
    30121 ccaatggtgt cgctaacctg agttgccagc gcctgggcat tggcgtttgg ccgataatcc
    30181 agctcactca cggctttcat tactgcttca cgcgtgtcgg cactgaccag cgtgctgtta
    30241 ttgagcaccc gggaaaccgt tgccacagag acgccagcct gacgcgctac atcacgaatg
    30301 gtgatcatat tcactacctg ttatgagatt gcagtaaatg actgcttgct ggcggctatt
    30361 ttgtcagcac tgaatacagg acttcgtgaa tcgagtcaca gcaatggaaa cggttacagc
    30421 cgttttgtta acgaatgtga tccagatcgt tatcttgatg ttttagatat tgccgttccg
    30481 gcagcacgca aagttaactg acgccagagc cattccaccg gcccctggcg gaagtaacgc
    30541 agccagataa cagagaagag gatattcgcc agccataccg gaataacaaa cgccagcagc
    30601 tccaggcggt caaaatgcat aaacaaaccg aggtggtaaa aaagcatggt acaaatcagc
    30661 gtttgcaata gatagttggt cagcgccatc cgtccgacgc aggcgatcgc aagcaccagc
    30721 ttaaagcggc tcaattgcgg ccagaagcca taaaacagcg acgcatagcc aatcgcctga
    30781 aacggcgcac tcagttcccg cggcatttga agtaagaagg cacaccagcg atacgcccag
    30841 tccagctgcc attgcagggc gatggcagga aggttaatgg tcaccccaat cgccaccagc
    30901 acaaaaccag tacgacgata gtgacgtaag ctgaactgcc ctttcagcca gccgctgcgc
    30961 atcagcgctg caccaatgag catcatccca gccagttgcc agccatactg tgcgcccagt
    31021 gccagtaaac tgttgccaac accatcggca cgattactga tcgcttcaac gccgccgtga
    31081 agcttccagt atttttcata caaaatagcc gatgcatccg gcgtccaggc gcggctggtc
    31141 tggctgtcgg aaatcagccc caataacagc aaaacgccaa gccccaccag ataaagcatg
    31201 acgcctgtat taaacaggct ttttaccgat ggcgcatcgc gcaccagccg ccagcagatt
    31261 aagcccacca gcccgtaagc cagcagaata tcgccgtccc agaacaataa accgtgaata
    31321 aagcccaata agactaacag cgttaaccgc gactggatcc agcgtctgcc acggggcagc
    31381 aacatttgca gtccagcacc aaacagcagc gcaaaaagcg tgaggaattt cacctgggcg
    31441 atgagatcaa gaaatgccca ggtccatgca tcctgcggcg taatagcacc gtaccaggcg
    31501 ggattgagat aagccgcctt tggtagccca aaggcgctga tgtttaatag caggatcccc
    31561 agaatggcga cgccgcgaac aaaatcgagc gtgacgttgc gctccatggt tcctgccttt
    31621 taatcagttg tgatgacgca cagcgcgcag aaactcgtgg cgcgtattct gactggattt
    31681 gaacaatcca ccaagagagg tcgttgttgt ggcactggtt gcatcgcgga tgccacgcgc
    31741 cttcacgcag taatgcaccg cgtcgatcga gacagccacg ttattggtgc ccagcagcgt
    31801 ttgtagcgca ataagaattt gctgcgtcag acgttcctgc acctgcggac gctgggcaaa
    31861 gaactgcacg atacggttaa tttttgacag accgatcacc gaatctttcg ggatataagc
    31921 caccgtcgct ttgccatcga tggtaacaaa atggtgttca caggtactgg tcagagtgat
    31981 atcgcgcacg gtgaccattt catcgacctt cattttgttt tcaatgaggg tgattttcgg
    32041 gaagttggcg taatccagac cggagaaaat ttcatcgaca tacattttag cgatgcgatg
    32101 cggcgtttcc atcaaactgt catcagccag gtcgagattc agcagctgca tgatttcggt
    32161 catatgacca gcaataaggc ttttgcgcgt ttcgttatcc atttcatgca cgggcgggcg
    32221 cagcggtgtt tccagtcctc gcgcaactaa cgcttcatga accagggccg cttctttact
    32281 gagtgatggc atttatgatt tctcctgcag gtgtgacgcc tccgccctgc gtgggggcaa
    32341 agttattaag ctgatttaca gcttgattat tgtgcgtgag gcggcgcaca taatccagta
    32401 ttcacagcga taattattgt aattgccgct gcctttcatc agcagatgtt aaaacatcgt
    32461 tatgcaaata cggaagtgaa agttactcac agcacattga ataaacggta tgatgaagaa
    32521 attgcaaaca acacaacaag gagccacgca tggaaatgct cgaagagcac cgctgttttg
    32581 aaggctggca gcaacgctgg cgacacgact ccagtacctt aaactgtccg atgacgttca
    32641 gtatctttct cccaccacct cgtgatcaca ccccgccacc agtgctgtac tggctttccg
    32701 gattaacctg caatgacgag aacttcacca tcaaggcggg tgcccagcgc gtggcggcag
    32761 aactggggat tgtactggtg atgccagaca ccagcccgcg cggcgaacag gttgccaacg
    32821 atgatggcta cgatttaggc cagggcgcgg ggttttatct taacgccacg caaccgccgt
    32881 gggcgacgca ttaccggatg tatgattatc tgcgcgatga attaccggcg ctggttcagt
    32941 cgcaatttaa tgtcagcgac cgctgcgcca ttagcggtca ttcaatgggt ggtcacggtg
    33001 cgctgattat ggcgctgaaa aatccgggta aatacaccag cgtttcggcc tttgcgccaa
    33061 ttgtgaatcc gtgcagcgtc ccgtggggaa tcaaagcgtt tagcagctat ttaggtgagg
    33121 acaaaaatgc atggctggaa tgggacagtt gcgcgctgat gtatgccagt aacgcgcagg
    33181 atgcgatccc gacgcttatc gatcagggcg ataatgatca gtttcttgcc gaccagttgc
    33241 aacctgcggt actggcagaa gccgcgcgcc agaaagcgtg gccgatgacg ctgcgtattc
    33301 agccgggata tgatcacagt tactacttca tcgcctcttt tatagaggat cacctgcgct
    33361 tccatgcgca gtatttactg aagtgaaagt ccgcccggtt cgccgggcat cttctcatca
    33421 gaagcgataa tccactgcca taaagtaacg acgtccgtct tcgttatagc tgtagtcgtc
    33481 acgactgaga tccttgtcgc caaggttcag cacgcctgcg cgcagtttga cgtctttagt
    33541 cacctgccag gccgcgccgg tattccagat ggtataaccg cccggtgttt tagccgtcgc
    33601 gctgtcggcg cgtttctgcc cggtatagtg accagaaaca tagaatgacc agtcttccag
    33661 cgccagcggt ttccagtcca gcgtaccgtt agcagtatgg aacggcagat cggatagcgg
    33721 tttgttttcg ccgttgctga catcacgacc atcgttgtag gtgtagttga tcgacagttt
    33781 ccattcatcg ttgaacggaa ttttcagttc ggtttccacg ccctgaatac gagctttgtt
    33841 aacgttgtag tagctaaata ccggtatgcg ccgtccgtta gcgcccgtct caaaaccaac
    33901 aaagttttgg tagcccggtg cagcgttgac gtcagacgta cggctgatgc tgatacgatc
    33961 tttcacatcg ttacggaaaa cggtaacgct ggattcaacc ccttccagcc agccttcttc
    34021 acccatgtag taaagcccca gctcccaact ttcgctggtt tctggtttca gatccgggct
    34081 acccacaatc ttacatgcgc cacggcagga attgctcgtc cagtcagggc taagttgcaa
    34141 cagagaaggt gctttaaatg ccgtcgccca gccccctttc accgttacgg tgtcggtggc
    34201 gttataaacc aggtaggcac gcggactcca gtgttcaccg taggtttcgt gatcgtccat
    34261 acgcacgccg gtcgtcagcg ccagcggctc gaagatccgc cattcatctt ccacaaacag
    34321 cgcgtactgg ctggcagacg ttttggagct ggttcccccg gtcaggttca ccgcatcgct
    34381 aagtttgtcg tgacgccatt caccgccaac cgtgagaaac tgattaattg ccgtcagcgg
    34441 caacgtgtat ttgccgtcga ccgtattgct ttcggaagtt atcgggctgc tgttgccagg
    34501 gtttttgttc tcgactttct caccgtagta tttcagttcg ctggtgccgt aatcccaacg
    34561 cccattatgg ctgacggagt agttctggcg ttccaggcgg tttttgtcca gcgagtcgga
    34621 atcacgatcc tgacggtcga aaccgtatcc ggcagtaaaa tcgtgatttt gattcggtgt
    34681 ccaggcaaat tcgacattgc cgtcgcggct ggagaatcct tcaatacgcg gcgtttctcc
    34741 ggtatcggtg gtcgttgagt tttgcgggtc atccttttca cgttttgcca ggctgccgta
    34801 agctttcatt cccagcacac catcaattaa tggtccactg gtaaagaact gaccgttata
    34861 ggtatcaccg cgatcgcgat gttcctgaat ggtggtatcg acggtaacgg tacccgacca
    34921 tttctgaccg atttttttgg tgatgatatt cactacaccg ccgagcgcat cggagccgta
    34981 tagcgacgac atcgggccac gaaccacttc aatacgttcg atggaatcga ccgggatcca
    35041 gttcagatcg aaatcattgt ggcggaagac ggcattgcgg gagttaacgc gtttaccgtc
    35101 gacgagaatc agggtgtagc tgctgtccag accacgaata ctaacgccct tacggttatc
    35161 cccttcgttc gtcagttgta cgccaggcac ttctttgagg acatccttca gattctgtac
    35221 cggttttcgc tgcaggtctt cctgggtaat gacgctgata ctggcgggtg catctttgag
    35281 gttttgttca acggaagatg cagtgacaac catcgtttcg ccatcatcat cgaccgctaa
    35341 cacaggccat gcacaagaaa tagcggacaa acacagcccg acccgtacga aagggttcaa
    35401 cctaaacatt ccatatctcc atgaggtaac tacgaaaata aaatgggtta tcgctcacat
    35461 cttcttcacg tccccttgcg tacggcagca tcgcggtgga cttatttgta tgcaggtgat
    35521 catcccagaa agccggaaca gcctctgatt tgtcagcctt ttttgattgc ggcgtaacga
    35581 taatgcaaac gataacaatt atcaatccaa atgttaaatt ttatatccgc atgatttgtc
    35641 agggaaatgg aattgaaaaa gccctctcgg ttgagagggc ttagcaagga agggaggaaa
    35701 cttatttctt atcgttctgc gggaacttca tttcgctgta gcgtacgaag tgagttcctt
    35761 taatcagctt gtagccgaac caaataatca ggaacagcgg gataccaata tacgttgccg
    35821 ctacgccgcc ccagtcaatg gtatctttca ggaacgcttc gtagttctgg cccaaagtga
    35881 taatcagaca cagaatgaat gcgaagatcg gccccagtgg gaagaaacct gaacggtacg
    35941 gcagatcgtt aatgtcgtgt ccctgcaata cgtaaccgcg acggaagcga tagtggctaa
    36001 tggcaatccc cagccaggcg ataaaacccg tcatcccgga ggtgttcagc agccacaggt
    36061 ataccgtctg gttgccaaac atggaggtca ggaagcacag accggcaatc accgtcgtcg
    36121 catacagcgc attacgcggc acgccaccac gcgacagttt agcgaaaatg cgcggcgctt
    36181 taccgtcaca cgccagggtg tacagcatac gagtagacgc atacataccg gagttacccg
    36241 ctgacagcac cgccgtcaga ataactgcgt tcatcaccgc cgccgcagag agcagacccg
    36301 cgtgctggaa caccagggtg aacggactaa cgctgatgtc tttaacatcg ttacgcagca
    36361 ggctcggatc ggtgtacgga ataatcaggc tgataatcag gatcgcgaac acatagaaca
    36421 acaggattcg ccagaacacc tgacgtaccg cgcgtggaat gtttttcgcc ggatcttcgg
    36481 actcgcctgc agcaataccg atcagctcgg ttccctggaa agagaagccg acaatcatag
    36541 ctacgccgat catcgccgca aaaccaccag caaacggcgc ttcgccgatt gtccagttgc
    36601 tccagcccgc aggctgcgcg cctttgaaga taccgataat catcagcacg ccaacgatga
    36661 taaagacaat aactgtcgtg actttgatca gtgagaacca gtattccgct tcaccaaagc
    36721 cacgaactga gatgtagttc agcaggaaga taacgccgag gaacaacgca ctccagatcc
    36781 agcccggtgt atccgggaac caccagctca tgaccagctg agctgcaacc aggtcaacgg
    36841 cgatagtcac cgcccagttg taccagtagt tccagcccag cgcgaagcca aagccttctt
    36901 caacatagtt ctgaccgtaa gtggcaaacg aaccggaaac cggcatatat gcagccagtt
    36961 caccgagact ggtcatcagg aagtaaacca tcaggccaat cagcatatac gagagcaatg
    37021 ccccgcccgg acctgcctga gaaatcgttg cgccagaggc aacaaaaaga cctgtaccga
    37081 tggaaccgcc aatggcaatc atcgtcaggt gacgcgcctt taattcacgg cgtaagcccg
    37141 gcgcttctgt ggttttagtt tcggaaacca tacaaaaatg ctatccatct taaaaatgag
    37201 gcgcgattgt agcagacgat tggcaatcct tccggcagaa atacacggtt ataagacacc
    37261 ttcatgatcg cccagggatt ataagtaaag cagccaatct cttttctggc gaatgcatgc
    37321 tgagggcgct gcgtcacgaa atcgacacag caccagcatg ttcttgtaca gcaacttaac
    37381 gcggtacatt cgcgggatcg caatagtcca gaaagcgccg tagcgcgttg gaaaggtgtt
    37441 tttgccgatg atgtatacgc cacaacgtac gcatcaggcg cgggagaggg accgcaactt
    37501 cacttaatgt gcctgcctgc aattgatctt taatcacccg tcgcgacagg caactaattc
    37561 ccaacccatg acgcaccgca tgtttgattg cctcggagtt acctaattcc atcgccatct
    37621 caaacttcgg taaatgtgac agcaacagat aatcgacaat ctcccgcgtg ccggaaccgc
    37681 gttcacgcag gatccacggc gcagcggcca gctgttctaa ggtgaccgga ccacgagcca
    37741 acggcgaagt cggcgcggcg aaaaccacca gctcgtcttc cagccacggt tcagaaatga
    37801 tttcagtgct gtggcacggt ccttcaataa agccaatatc aacgcggaaa tccagcaccg
    37861 cttgcatcac gtcctggctg ttcccaacgc taagttcaat cggcaactgc ggataatcat
    37921 ggcgataacg ggcgataact gcaggcagaa tgtagttacc gatggtacta ctggcataga
    37981 tacgaatcgc gccgttgtct tcgcgaaaca gttgttcgat ttcaaccgcc tgttcaagca
    38041 atgccaacgc acgcggatag agcagccgcc cgtgttcatt aacaaccagt cttttcccca
    38101 cgcgatcaaa cagttgcacg ccaagctgcc cttccaggtc ggtcaaggct gcgctcactg
    38161 ctgattgcga caacgccagc atcaccgacg cctgggtggt tgatccactt ttcaatactt
    38221 ctgcaaaaac ttccaactgc cggagggtga tgtgcat
//
LOCUS       DAZFWL020000033        38241 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFWL020000033 DAZFWL020000000
VERSION     DAZFWL020000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 38241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.033"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(271..2292)
                     /gene="fyuA"
                     /locus_tag="ACHKNP_RS09035"
                     /old_locus_tag="pgaptmp_001807"
     CDS             complement(271..2292)
                     /gene="fyuA"
                     /locus_tag="ACHKNP_RS09035"
                     /old_locus_tag="pgaptmp_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HGU4703993.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIEDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPDGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
     gene            complement(2423..3988)
                     /gene="ybtE"
                     /locus_tag="ACHKNP_RS09040"
                     /old_locus_tag="pgaptmp_001808"
     CDS             complement(2423..3988)
                     /gene="ybtE"
                     /locus_tag="ACHKNP_RS09040"
                     /old_locus_tag="pgaptmp_001808"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HGU4703994.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
                     QPDVSTTALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHN
                     FPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWE
                     DSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHATILH
                     SQGRPLSPLDEICIVDQDENDVAPGETGQLLTRGPYTISGYYHAPAHNAQAFTAQGFY
                     RTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTL
                     LGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRL
                     TALAVDRYRHSAQ"
     gene            complement(3992..4795)
                     /gene="ybtT"
                     /locus_tag="ACHKNP_RS09045"
                     /old_locus_tag="pgaptmp_001809"
     CDS             complement(3992..4795)
                     /gene="ybtT"
                     /locus_tag="ACHKNP_RS09045"
                     /old_locus_tag="pgaptmp_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HGU4703995.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            complement(4792..5892)
                     /gene="ybtU"
                     /locus_tag="ACHKNP_RS09050"
                     /old_locus_tag="pgaptmp_001810"
     CDS             complement(4792..5892)
                     /gene="ybtU"
                     /locus_tag="ACHKNP_RS09050"
                     /old_locus_tag="pgaptmp_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HGU4703996.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDMVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLYVADHQQNPHSLWRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSNLAGEHPPEACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            complement(5889..15371)
                     /gene="irp1"
                     /locus_tag="ACHKNP_RS09055"
                     /old_locus_tag="pgaptmp_001811"
                     /pseudo
     CDS             complement(5889..15371)
                     /gene="irp1"
                     /locus_tag="ACHKNP_RS09055"
                     /old_locus_tag="pgaptmp_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
     gene            complement(15459..21566)
                     /gene="irp2"
                     /locus_tag="ACHKNP_RS09060"
                     /old_locus_tag="pgaptmp_001812"
     CDS             complement(15459..21566)
                     /gene="irp2"
                     /locus_tag="ACHKNP_RS09060"
                     /old_locus_tag="pgaptmp_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HGU4703997.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDVARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLQPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     ALAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAYWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLVGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPDFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTQRPRPVRRRQRRI"
     gene            complement(21757..22716)
                     /gene="ybtA"
                     /locus_tag="ACHKNP_RS09065"
                     /old_locus_tag="pgaptmp_001813"
     CDS             complement(21757..22716)
                     /gene="ybtA"
                     /locus_tag="ACHKNP_RS09065"
                     /old_locus_tag="pgaptmp_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HGU4703998.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHDGFNHEQKPSTLWLQGQSLVMLSLVLDEQREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            22973..24685
                     /gene="ybtP"
                     /locus_tag="ACHKNP_RS09070"
                     /old_locus_tag="pgaptmp_001814"
     CDS             22973..24685
                     /gene="ybtP"
                     /locus_tag="ACHKNP_RS09070"
                     /old_locus_tag="pgaptmp_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HGU4703999.1"
                     /translation="MALAGLAALTSLGALLFLVWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLQTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSEQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFVDTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            24672..26474
                     /gene="ybtQ"
                     /locus_tag="ACHKNP_RS09075"
                     /old_locus_tag="pgaptmp_001815"
     CDS             24672..26474
                     /gene="ybtQ"
                     /locus_tag="ACHKNP_RS09075"
                     /old_locus_tag="pgaptmp_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HGU4704000.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIACPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            26467..27747
                     /gene="ybtX"
                     /locus_tag="ACHKNP_RS09080"
                     /old_locus_tag="pgaptmp_001816"
     CDS             26467..27747
                     /gene="ybtX"
                     /locus_tag="ACHKNP_RS09080"
                     /old_locus_tag="pgaptmp_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HGU4704001.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFRREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFMMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     LHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            27775..29079
                     /gene="ybtS"
                     /locus_tag="ACHKNP_RS09085"
                     /old_locus_tag="pgaptmp_001817"
     CDS             27775..29079
                     /gene="ybtS"
                     /locus_tag="ACHKNP_RS09085"
                     /old_locus_tag="pgaptmp_001817"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HGU4704002.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVMGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLSENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(29273..30188)
                     /locus_tag="ACHKNP_RS09090"
                     /old_locus_tag="pgaptmp_001818"
                     /pseudo
     CDS             complement(29273..30188)
                     /locus_tag="ACHKNP_RS09090"
                     /old_locus_tag="pgaptmp_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(30350..30425)
                     /locus_tag="ACHKNP_RS09095"
                     /old_locus_tag="pgaptmp_001819"
     tRNA            complement(30350..30425)
                     /locus_tag="ACHKNP_RS09095"
                     /old_locus_tag="pgaptmp_001819"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30390..30392),aa:Asn,seq:gtt)
     gene            complement(30526..31323)
                     /gene="mtfA"
                     /locus_tag="ACHKNP_RS09100"
                     /old_locus_tag="pgaptmp_001820"
     CDS             complement(30526..31323)
                     /gene="mtfA"
                     /locus_tag="ACHKNP_RS09100"
                     /old_locus_tag="pgaptmp_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HGU4704003.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            31417..31506
                     /locus_tag="ACHKNP_RS09105"
                     /old_locus_tag="pgaptmp_001821"
     tRNA            31417..31506
                     /locus_tag="ACHKNP_RS09105"
                     /old_locus_tag="pgaptmp_001821"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31451..31453,aa:Ser,seq:cga)
     gene            complement(32175..32825)
                     /gene="zinT"
                     /locus_tag="ACHKNP_RS09110"
                     /old_locus_tag="pgaptmp_001822"
     CDS             complement(32175..32825)
                     /gene="zinT"
                     /locus_tag="ACHKNP_RS09110"
                     /old_locus_tag="pgaptmp_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HGU4704004.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPIFQKKADADKTKTFAEIKDYYRKGYV
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(33082..33717)
                     /gene="msrQ"
                     /locus_tag="ACHKNP_RS09115"
                     /old_locus_tag="pgaptmp_001823"
     CDS             complement(33082..33717)
                     /gene="msrQ"
                     /locus_tag="ACHKNP_RS09115"
                     /old_locus_tag="pgaptmp_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HGU4704005.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSV"
     gene            complement(33718..34722)
                     /gene="msrP"
                     /locus_tag="ACHKNP_RS09120"
                     /old_locus_tag="pgaptmp_001824"
     CDS             complement(33718..34722)
                     /gene="msrP"
                     /locus_tag="ACHKNP_RS09120"
                     /old_locus_tag="pgaptmp_001824"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HGU4704006.1"
                     /translation="MKKNQFLKESDVTAESVFFMTRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            complement(34831..35244)
                     /gene="hiuH"
                     /locus_tag="ACHKNP_RS09125"
                     /old_locus_tag="pgaptmp_001825"
     CDS             complement(34831..35244)
                     /gene="hiuH"
                     /locus_tag="ACHKNP_RS09125"
                     /old_locus_tag="pgaptmp_001825"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HGU4704007.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            35377..36048
                     /gene="hprR"
                     /locus_tag="ACHKNP_RS09130"
                     /old_locus_tag="pgaptmp_001826"
     CDS             35377..36048
                     /gene="hprR"
                     /locus_tag="ACHKNP_RS09130"
                     /old_locus_tag="pgaptmp_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HGU4704008.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            36048..37406
                     /gene="hprS"
                     /locus_tag="ACHKNP_RS09135"
                     /old_locus_tag="pgaptmp_001827"
     CDS             36048..37406
                     /gene="hprS"
                     /locus_tag="ACHKNP_RS09135"
                     /old_locus_tag="pgaptmp_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HGU4704009.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(37514..>38241)
                     /gene="hchA"
                     /locus_tag="ACHKNP_RS09140"
                     /old_locus_tag="pgaptmp_001828"
     CDS             complement(37514..>38241)
                     /gene="hchA"
                     /locus_tag="ACHKNP_RS09140"
                     /old_locus_tag="pgaptmp_001828"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HGU4704010.1"
                     /translation="KPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLH
                     AAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKIADVVVSLNADS
                     EYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLN
                     GYSICAFPDAADKQTPEIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTG
                     DTPFAANALGKLAAQEMLAAYAG"
BASE COUNT         8400 a        10146 c        11020 g         8675 t
ORIGIN      
        1 tgcttttata ttctggagat gtgccttgta tataggattg tttgttggtt gtatttttta
       61 atgttgcact gttgtgaatt tttattttga tatgattatt tctttagtat aaggagagaa
      121 aggaacgaga aagttaaatc tatttatcct aatcgcaatt tcatttttta aataatcact
      181 atggattaat attttttgcg actctgctga gttactggtt ttaactagtg gtgtaaaagg
      241 gatacctttt cggtatccct tttacaataa tcagaagaaa tcaattcgcg tattgatacc
      301 gacggtgcga cccatattga cctgcgcgac ggcgctgctg ccgttcatgt agccataggt
      361 acggtaacga cggtcgaaca ggttatcgac atagacggaa atgttcatcc gttcagtcgc
      421 ctgccagccc aggctgctgt ccagggtggc ataggtgcct tgccgcaact ggttgtcgcc
      481 atcgaaataa tgcggcccga ccagattaac cgccagtcgg ggcatcagtg cgccatagcg
      541 cgtatcaatc acgccgttca cgctgcttcc cgcgccataa cgtggtacga acggcacccg
      601 gttaccgtga tacaactcac tgtcattggt gaattcggaa cggatcacgt tgccattgat
      661 atcccatgac cagcctggcg caaaccgcca cttcgcttca agctcaacgc cggtggcgtc
      721 ggctttaccc gcattgctta atgtctgcat cccgtccggg ccagagtaaa gctgcatgtc
      781 tttggtgtgg gtataaaacg tcgcggcttg cagcgtgacg tcagcggttt cgtagcgggt
      841 gccaagttca tagttgatgg atttctcggc gacgaacggt ttggcatcaa gacccgcagt
      901 aggcacgatg ttgtacccgg aaggtttata tccctgggct acacgggtat acactctcca
      961 gtcatcggtc agcatatagc ctgcggatag ctgcccgagc acctgatcgt cattgctctt
     1021 accctggtcg ccaaacgggt tgccgagcat gctgccgtga tattgtgtac tggatttatc
     1081 atgcgagaag cgcacgccgc cgccgatatc aaaacgatcg gttaaatgcc aggtcaggtc
     1141 actgtatgcg gccagcgttt cagcggtggt atagccggta ctgcttaaat aaggcattgt
     1201 cggcatgtcg taggctgaat ttaacttctc gcgggtgttc tgccggtaca gcccaaacac
     1261 catatcaacg gtacgcgcat cgcccagggt tgcggcgcgc agctcctgca catcctgatt
     1321 ccagcgctga ggcatattga cgattaacga accggaaggg aaggtgcgcg aataatgctg
     1381 ctgctgccag gcgctgatca ggttgaaaac ccagtcatcg gtggtgtatt tcccactcag
     1441 ggtttggctg tcagtgcagc gccgcatgta cgggtctggt gaaccatcgc tgatcgacag
     1501 cttacggccc ttaatatcat tccatcccac ataggcatcc tgggtggcgc gagtacattc
     1561 gcgtgaggcg gcaaagccca tttcccaggg ctgatcgtcc ggcgccagac gcagtttcac
     1621 attccctatg ctggcgcggg tgccgcctaa gtcatcgctt cccgtcgcgg ggttaatcat
     1681 gtcgccgtca tcaacctggc gtaacagggt gacgctgccg tacagcaggc catcctcaat
     1741 ggggccgctc aggttgaact tacttcgata actgtcgcgg ctactgacgc cgccttcaat
     1801 atagccgcgc ggcgtgctgt ccggctgctg ggtgacgatg ttgatgatcc cgccctgagc
     1861 gcttttgcca tataacgttc cctgtgggcc tcgcagcaac tccacgcttt gcacatcggt
     1921 aagcgcctgg atggtgttgg tggaaagctg agggacgcca tcgacataca gggtgacggc
     1981 ggggttatag aagtcctgcg ctgaagagac gccgcgtagc gagatcgtcg aaaaaagcat
     2041 gttgccgcta ttttcaatat tgagcccggg caagactctg gggagtttgt cgctggcggt
     2101 gacgccggcg tcgcttaatt ccggcgcgct gacaacagta gacgagacgt tgttggctga
     2161 tgccgagcgg gaagattgtt tactggcggt aaccaccagc gtgctttcgt cttgctgtga
     2221 agtctgggca ttagcaatgg cggggagcaa taatcccccc aaggccagag gataaagccg
     2281 tgtcattttc attgttgtga gtccctgtaa gacggcgaaa cgccaaaaat accgacaatg
     2341 gtgaattgaa aatgataact atttctaata gtggtgctac ccgaaacggg tttatttcaa
     2401 cctgtttcgg gtcggtttgc gcttattggg cagaatggcg ataacggtcg acggcgagag
     2461 ccgtcaggcg ttttttgtct atcttgccga cggcggtgag cggccagtgg tccagaaact
     2521 cgatttggtc aggaattttc cacgcgctga gccccatacg ggtcagttgt tgacgcaact
     2581 gctgatagtc agttggcacc tgctgcgcga tgataaacgc gcaaatccgc tcgccaagca
     2641 gcgtgtccgg cgcggcgacc accgcgcaat cttgcacttc cgctaaacgc agcagtgccg
     2701 attccacttc agccgcggct attttttctc cggcgcggtt gatctgctct tttatgcgtc
     2761 cctcaacgtg caggttcccc acctcatcca gcctgacatt gtcgcctgtg cggtaaaacc
     2821 cttgcgcggt aaaggcctgc gcgttgtggg caggggcgtg gtaatagccc gaaatggtat
     2881 aagggccgcg cgttaacaat tgcccggttt cgcccggcgc gacgtcgttc tcgtcttgat
     2941 caacgatgca gatttcatcc agaggggaca acgggcgccc ctggctgtgg agaatggtgg
     3001 catgcggatc gtccagccgg gtaaaacaga gcaggccttc cgccataccg aaaacctgtt
     3061 gcagggtaca gtcaaaggtg gcgataacct gctcagcaag cgtcgggtcg agccgggcgc
     3121 cgcctgcctg aatgacgcgc agcgacgaaa ggtcgctgtc ttcccactcc ctggcctgca
     3181 cccataattg cgccagcgcc ggaaccaggg cgacgtgagt cactctttcc tgcgcgatta
     3241 aaggcatcac ctcatcacag ctggcgctgt cggtcagcac cacttttccg ccgcaggcaa
     3301 gcgttcccag aataccgggg caggccagcg gaaagttatg cgccaccggg aggacggcga
     3361 gatacacgct ctgttggctg atgccgcaca gttcagcaga agcgctgaag ttatagctat
     3421 agtcggcatg tcggcgcggg atgagtttgg gcgtgccggt tgtgccgcct gagagcaaca
     3481 acaacgcggt ggtggaaaca tcaggctgtc gctcaccgtg aagggagaag agcggcgtaa
     3541 aatcgtcgct catggtctct ccagcgacca gaacatgacg caagcaggcg tgtttgtgtg
     3601 ccatctgtcg ggccagctct gcgtggtttt ccccgtgaat aacgtaagcg acgggttgcg
     3661 ccagctcaat cagcgcgtcg atatccagcg cccgttgcga gggcatcgcc agcacgggga
     3721 taacgcccag tcttaacaag gcgaacagca gggtaacaaa cgcgttgtcg ttgggaagct
     3781 gcacaattac atgctccccc gaacgcaaac ccagtgatga cagacctgcg gcaagttcgt
     3841 caacttgtgc atcaagcgcg ctgtaggtta acgacccctt tgcatcaacg acggcaattc
     3901 gcgaggcata acgggccgcc cagtggcgca actgttcggc aatgggtaag ggatactgtt
     3961 caatcagaga ttcaaaggaa gaattcatgc gtcacctttc tgaagtactg ggctgttttt
     4021 gccacgcggt cattgcagaa aatgcgtggg gaaaatggcg gacaatctgc gtaaaaaagg
     4081 accgggcttg ttgaatggga tagaagtgat cgccgtcaat caccaccggg cctgtaacgt
     4141 ggctcagcca ctgacgccag gcatcgacct gctgccagga ggcttcgcga tcgtggctgc
     4201 cgcacaataa cagcgcaggc gtgcgcagcg gcggacagac gtcgggcgag tcgtagtgat
     4261 agctctcggt ggcgtaaaaa tcagcgcgca gaagaggaag aaacagcgac attaattcct
     4321 ggttttcccg cagttcagga gaacatccgc caatgtctat cagctcagcg ataaagtcgg
     4381 catcatcgcg atggctgagc tggcgttcag aatgcagatg cggggcatgg cacccggaaa
     4441 taatcagtcc ttgtggcgca agcccccgtt gctccagaag tcggcaggtt tcaaacgcca
     4501 cctgcgcccc catgctgtgc ccggcgagta aaagcggcgt gtcaggcgat acggatgctt
     4561 ccagctcgtt cgccagcagt gccgccagtt gtgtaatgct tctgagcggt tccagatggc
     4621 gaaggcgatc gcgccccggc caggtcacca gagaaagcgc gcaatcagcc agttgctcag
     4681 cttgccagtg gcgaaacgcg ctactgctgc cgccagcgaa agggcacatg accagatgcg
     4741 cagtattgcc gttccgggcg ggccacagcg ggatgcacat tgcagattgc gtcacagcgc
     4801 ctccttatca tcatcgttgt agaaaccggc taaccggtcg tggtgcggcg gcgtcagacg
     4861 tctgatctcc gcattgccgg tcttacgtaa aatctgctgc cataaacgtg ataacgcgat
     4921 ctgatgtacg ctttggcagg cctctggtgg atgttcgccc gccagattgc tgcgcaactg
     4981 gtgtagcaac cagctgacgc cctccggccc gacggtttcg cagcagtcgc gccagctcag
     5041 gggcgccgcg ctgcgcgtca gacccggcag atcgcgcagg atctccggtc tgcgccagag
     5101 actgtggggg ttctgctgat ggtctgcgac atacagactg gatgaccaga tgacggggcc
     5161 gtagctcgcc tccagcgaaa gatgaccttc cggccagccc agcagcaggc ggtgcatgat
     5221 aaggctatgc atatctggat cgtcaggatc gagatagcgc tgaagcagca ggcaggcttc
     5281 tccctccggc cagaagagtc tcagacagtg aaaatcgcta aagctgccaa ccatgtcgca
     5341 ctccacggcg gcggcatcaa cgcccagagc cagcaacaac aaatccagag tggagtagag
     5401 caactgtcgg ctggtggtgg cgtggactac cggcggcgtc ttagccaggc agcgacgcag
     5461 ctgctgcgca tcgcgaagcc atgtgcgtcc cgcgcgcgta tgcggataaa acgtgtttac
     5521 ccaatagcag cagccctgtt cctgcgccag cgtttgtaag gaactgatgt catccgggtg
     5581 gaggggatgc tcctgaatga cgtgtacgcc gcgcgtcaga aagtgtctgg caagctgcgt
     5641 accggttcct ccggcgacgg tcgaacgcac gacaatacag gcgatatccg gcatcctggt
     5701 tatctgttcc ggcgaggtat acagcggaat gccaaacgca tgagccagct ctcttgaacg
     5761 ggcgcttccc tgcgccagca ggccgaccag ttccagcccc tccgggggct gcataaaggc
     5821 attcaggtac atttcgccaa atttggcgcc cacaatcagt acgcgttgtt ttggggaggc
     5881 ggacggcatc ataacgtgtt ctccggttgc gttgaggttg cgcaaagcca gcgcgtaatg
     5941 tgttgcgcac aagcctgaac gtggggggct tccatcatga tttgccagtg gctggcttca
     6001 atcacagcgt cgtcggcgtt gtttatccag ccctgcaact cggtttctgc tggcgtccag
     6061 cgcgcgggtc tcccggcggc atacaccatg agacagggga ccggaacgct ttcgcccggc
     6121 gtatgctcag tcagtaaacg cagcagatgc gcgatgttgt cgagccacgt ttctgccgct
     6181 tgcagcgtca ggttttgcga caccataccg gccgttttag cgaggctgat aaagtcggca
     6241 agctggctgt cgggcgtctg ctggtcgaaa tgttcgggca gtgccagagg cgtttctcct
     6301 tcggctaaca ggcgcagcag ggccgcccgg ttttcgcaac agaaatcctg tcggcacacg
     6361 ggatcgatta acaccatccg aacctgcttg cctttggcgt acaggcgctg tgcggcgccc
     6421 gccgcaagaa acgcgccata cgaccaaccc gccagcacat aagggccgtg aggctgctga
     6481 cgacggatgc acccgacata ctcatcgatc atctggtcga gcgtagcaaa gcgctgcggc
     6541 gattttgctt gcaggccgaa aacgcgcctg ttcaacgcgc tagcgagcgg cagccaggcg
     6601 ctgatatcgc cgtctgaagc gtggaagaca aacagggttt cctcaccgtc gccctggcaa
     6661 aggggtatcg ggttgtcctc tcctgacgta gccgcctgta gatgggcgca gaagtcgctc
     6721 agcgtcgaat ggttgaacag atcctgaagg ttagccctgg caatccctct ccggtttagc
     6781 tgcgcgacca tccttgtcgc catcaggctg tcgccgccca gttcgaaaaa gtcgtgatgc
     6841 cttgccacgg ggcgagacag cagcgattgc cagagttcag ccacctgttt ttccagcgcc
     6901 gaattccccc cggcaactgg cgtgggtttg gctggctcag tcggcgcatc agacggggca
     6961 tgcagcggcg taaataacgc ttctctttgc cggatctgta aaatgggcag accggtgcca
     7021 aagcgctgct gtaaatagcg gctcacggct tttttatccg ggcgatttac gccaggcgaa
     7081 cgcgctacca gcagatgctg gcgcagcggc gacgattcct gcgcgggcca cgccagctcg
     7141 tttgcaaacc cggcctgaac gagaacctcc tgccatgcgg agcgggtgag catcggtttc
     7201 tcatcccggc ggcggaaatc gcggtatccg cttaatccct caataaagcc cacgctcgcc
     7261 agctggaata cgctgtttcg ctccgtcgct tcaacgatca gcaaacgtcc gccggctttc
     7321 agcaacagtt ttaatctgcg caacgtctgg acgacatggc tggcgtcgtg gagcacattg
     7381 acggcaacga tcaggtcgta acccgcctcc gggtgggcgg tgaaatccag cggctggttg
     7441 atgtcgaaca aggcataaga cacctgcgat tcatcatgga aacgggctct ggcgtcattg
     7501 aggaactgcg cggagacgtc cgtgaagtgg tacgactgcc gcgtggcctt cagcaccggc
     7561 gcggtagtgg ctgcggttcc ggcgccaacc tccagaatcc gttctgcgct gcacaaggcg
     7621 gcaatctgcg cggtatagcg attcagacag gcgctggcgg ggttgtcgcg atacagcgcg
     7681 tcggtaacgc gatgctgctc gttgaacagc aattccagcg gagaacactg cccggagaag
     7741 agggcgtcgt gccgggcgat gcaggtttcc agatactgcg ccagcgcctg gctccattgg
     7801 ctttgcgggc acgcttcctg aggctcagga atctcgctga gcggaatgcg gcagcgccag
     7861 ctttcacctt cgcggattaa ccactctctt tccgtcagac actgtagcca ctggcgcagc
     7921 agacgctggt gagacgcttg cgcggacagc gcctgtacta ttgcgctaaa gcgatgggcg
     7981 atctccggcg tggtaaacag gccgtgacga tttagcgtag ccgcgatccc ctgtagcgca
     8041 cgcgcctcca gccagcgcca ggtcgcctcc agctcatcaa gctgcgcctg ctcaggcaac
     8101 ggcagcgcca gttgggtaag ttcgtgctca tcaggcatcg ccgctgatgg cgaaggatcg
     8161 tcggccatca cgatatccag cgtcaacgcg ccctgttcgg ttatttccgc tctggcctgc
     8221 cggatgccgg ggtattcgag cgtcgcctgc tcaatatccc gcaggtcgta gtccgcctgc
     8281 gcgttcagcg gccagcgtcg gcggggaatg tgcccggcga tgccgctggc gagagtcttc
     8341 aggctttcag gggcggcgat gacggcttgc aggatggctc aatagtcatt aaacatcgcc
     8401 tcggcagcgc cgggttccag cacgttgtcc atgcagtacc agctaaacat caactcgccg
     8461 tcgctctcca tgacctgatg atccagccag acctgcggcg tttgcgtgaa tacatagcag
     8521 ggttcgccga acagatggct catcgcctga tcgatagtca tgccttccag cgtcatcccc
     8581 agcatactgg taaacactac cggcatcagc ggttgacgtt gtgatccgcg caggcggccc
     8641 agctcacgga tcacctcaac accgttcatt tcactgtgcg ccatattttg ccagaggcgc
     8701 tgttgggtct gttgcatctg ctcttgcaac gtcactggcg ctgagaagtt aaaatcaacc
     8761 agcgtgacgg aggtaaaatc accaatcaac tggttgattt gcggatggat cggctggcga
     8821 ttgaagaacg tcaggttcag cgtaaatgtt gtggtacggc tccagcgctc aagggtggcg
     8881 gcaaacagcg tgagcagcgc ggcagacggt gtgacgcctt gctgctgcca gcgctgtttc
     8941 acggcctgcc attctgtctt gccgatcgtc gatttgaagg tggtgaagtg tggcgtttcc
     9001 gggggcgtct caaccatcgg cagctctggc gctaagggca gttgcggcag tttttcctgc
     9061 cagtaatccc aggcatcgtg ccatgccgat gtctttcggc gcgcctgttc agccatcaca
     9121 taatcacgga aggtaatagc gagcggcgcc agcgtttcac cgcgccagac ctgcgccagg
     9181 tcgtccatca tgactttaaa actctgcaca tcaaactgca aaaggtcgag gttcatatgc
     9241 agacggtaat ggcaatcgtc gatttcgctg accaccagct caaaaagagg ccactggtcg
     9301 gcaggcaaaa cgcgatagct cagttcatgc cgccgttttt ccagcgcgat gcgctgttct
     9361 tccggggaaa gcgcgcgcag atcgtcacgc gggatgtgat actccggcgt tgtcgccagg
     9421 attcgctgct gcccgtcggc atcaaccacc atacgcaaca tatcgtggcg tgcgatgagc
     9481 tggttccatg ctttctccag tatggctaga tcgaactcat cgtggcgttt atcccactca
     9541 aacaggacgt gacaggcgac gccgccatag ccaatgaggt gggttcgccc cagccagtag
     9601 gcgtgctgaa tgggcgtcaa agggaagggc gcataacgct cgtcggcgtc gtgccgcaac
     9661 acttccggct gcgaaacggc aggcgtcgcc tctggcttag aacagataag ctgcgtgagt
     9721 ccatgaggag acagatcctg ccacgcccgt tccgcattga tgcgtacgcc caggtagtgc
     9781 tgaatatcgc tactgagttc aaggaagagc agcgagtcca tgccgagttg caacagatcc
     9841 tggtttggat gcagtgacgc cggatcgctc agccttagct gaaccgcaat ccgctttttc
     9901 agccatgcca tcaccgctgt ttcatcgctc aggctgccgt taaaggcgtt gtcatcagca
     9961 ggaggaatga ccggcgttgc ggctttttct gtggcgctga tgttaaacag agcctgttgt
    10021 aacgggggca ttttgtcggt aaaaacgcgc atcgccagtc gccacggggc gccgcgcatc
    10081 accgcctgtt ccaggtgcca gcagccttcg gcatcgctta acgcgcccat acctcggctg
    10141 gcgagcgtcg ccagcatttc cggcgtggcc gcccgaccgc tttctcccca tgcgccccag
    10201 gcgacagaga gcgttttcgg cgcatcaagg gtggaaaact gctgggccag cccgtccagg
    10261 tagccgcagg acagcgcatg ggcgctctga cccggcgcgc cgagggtggc ggcagcggaa
    10321 gagtagagaa taagatagcg tccgtcgtgg ttgcgcaggg tttgcaacag ctggcttgcc
    10381 gcctgcgctt ttaccgcgaa aacggcagcc agctggtggt catcaagctc ctgcaagggc
    10441 gcgtcagcca atacgccagc ggcatgaatc gctccggcaa tgccgccgtt ggccgccaga
    10501 tcgtcaagaa ccgtggccag ttgcccggca tcgcccacat cacaacggca gacgcgcgtc
    10561 tgcccgccct ccacgtcgcg tagccatgac tcatccacgt gcgatgccag cagggcgatg
    10621 cgtcgcgccc ctttttctct gagccagttc acggcaaggc ggcctaatcc gccaaacgct
    10681 ccggtcacca gatgccagcg gctatcgccg gtaaacacgt ttgccggtaa ttcagctgcg
    10741 catcccgtat tgggcgccag tgaagggagc caaagggtgt caccccgtgc ggcgagccag
    10801 cgctgtgata gtgagactgc gctcaaccct tgatgcagcg tttcccacgg ggtgttttcg
    10861 gcgagatcga tggcggcaag caaccgttcc ggctgttcgt tggccgcgac gcgaagcaag
    10921 gcccatagcg cgtgatggga tgcagagagt gcttcatttt cctcgactcg ccacgcgcgg
    10981 cgagtaacca caatcaatcc ggctgcgctg gcggtgagcg ctgctatcac tttctccgct
    11041 aaggccagcg tatcctcgct ctcctcaaca atcatcagtc gtgaagaggc gtgcggatcg
    11101 tgaatgatgc cgtattgcgc cagctcgtcg ctgcgcgcaa gcgtaccggc gctgaagcta
    11161 aaggtaagcg gatggtcaat gctggcgacg ttgaggggcg tccagcgcca ttgataatgc
    11221 gtttgcggcg caggaagagg cgcggcgggc gatagcggca gccactcgcc agcaggatga
    11281 cgggcttcaa cgcgcatctg accgcgggaa aaggcttcgt tttgccagct taagcgaacc
    11341 ttccccagcc actccggggc ggcgacgggg ggcggttcca caggcagagc gtcgccatga
    11401 cgctgtgaaa gcagacgcca ggcctcctgc cagcgggcgt taaactgttc gggctgacct
    11461 gcgcaatcag accagtcggc gagataatca ccgttatccg tcagcgcctg ccccaacacg
    11521 ggttctgatg gcgcggtgaa tgttacggcg ctaaccgcct gaccgggcag cgtggcgaga
    11581 atgatatgtt cgctcatccc ggcggtcggg ctgccatcct gcggtagcca cgccactttg
    11641 ctgaatccgg cgtggcggca ctgttgttgc cactgagcgg tggtgaggaa taactcacct
    11701 tcgcgggcgt cgagatcctg tagcggaaga accagcgggc cgaaaacgaa gtcaaacaga
    11761 cgcattggct gggtgatttt gcgcatcagc aggcgcccgc ccggcttgag cagggggcgc
    11821 agattatcga gcgtgcggcc aatatggcgg gtggcgtgaa tcacgttcgc tgccacgata
    11881 agatcgtaag actgtgcctg gaaaccctga gactgcgcct ctttttcgag atccagctcg
    11941 ctatacttca caaaatcata gtcggcgaat ttctgctggg cgcgacgggt gaacagcgcc
    12001 gagatatcgg tgaaatggta ctccagtgcc ggaacgccgt tgagttccgg cagcagccac
    12061 gcggtggtgc cgccggttcc gccgccaact tcaagaatac gcaacgactg gcggggctga
    12121 cgcgtctgga caatgccgcg taataccccg gcggcgattt ggttgaaata gcggccaaag
    12181 ctgaattcct gatacagcac ttccacgccg tcggaggcgc tttgcgggaa gataatcgcc
    12241 accggttctt ccgcgccgct catcatttca tataaccgat cgccggcacg ggcgatggtg
    12301 tcgggaatag cctgaaaacc ttcacaataa ctggcaagtt ccgtcagcag tgattcccgc
    12361 tgttgatgtt caatggggcg ggcgcggacg tatcgcccgt cggtgcagcg gtaatcgccg
    12421 tcgaccacgc agttattcag caggcgctgg agtagctgct ggtagcgggg cagcagacgt
    12481 ccacggcgca tgatggtcat ggcgtccacg ccgttctcaa tggcatcgcc ggtacagcgt
    12541 tgtaccagtt gatcgacgta gatggcgtgc agtcgcgtgg cgcactgttt aagcgcttcc
    12601 aggcgaggga gatcgagcgc tgtcgcggcg cgactcgcca cctccagccc ggcagacagc
    12661 gctgcgtcgg caggctcgca ggccggggag acgcgctctt tccagtaacg ttcagtatca
    12721 aacggataac atggcgcagc gatacgttgt ccatcgcccg ccagcaggtc ggcccacggt
    12781 agggcgacgc cggcagcgta aagctggagc agggcctgat tgaggacatc gctcgcctct
    12841 ttgttacgcc gggcgctggc tatccagtat gcgttatcgc ggtattcgcg ctgcccgcaa
    12901 gcaaccaact gggcatcggg ccccatctcc agaaaaacgc gggcgccgag ctgatgcgcc
    12961 acctgaatac tctggataaa acgcaccggc tggcgcatgt gtcggcgcca gtaatccgcc
    13021 tggttgagcg ttgactcatc aatgacgtcg gcggtgagcg tggaaataat cggtatttgc
    13081 cccggctccg cgtgcagtcc cgcgcaggcg tcctggaacc gatcgagtat cggctccagt
    13141 aaagcggagt gcgccgcacc ggttacgctc aggcgacgat agttaatgtc atgctgcgag
    13201 agcgtggcgc aaaataccgc gagacgggct tccggcccgg aaaataccgt atgttgcgta
    13261 ccgttgttgg cggcgagatc cagctcaaac tggcgagcca gcggcatcag cgtgtcttcg
    13321 tctgcaaata ccgccaccat cgcgccgctt gcgcactgct gcattagcgc gccgcgccga
    13381 caaaccagtg gcatgacctg ttcaatcgta tagtgtccgc agacaacggc agcggcaaat
    13441 tcaccgacgg aatgcccaat ggcgaagtct ggcttcagtc cttcagcacg ccagtgcgcc
    13501 gccatcgcga tttcaaacgc gacaatcgcc ggctgcgccc aggccatatt gtccagctgc
    13561 gccgaatcgg ggttaaacat cgcttcgcgc agtgatggcg tgagcatttc gctacaggcg
    13621 gaaaaacagc gatccagcgt gtcggcaaac gccgttgagt gctggtacat cgtttggccc
    13681 atagtgtgcc agtgcgagcc ctggccggta aacagccaca cctgcttgcc gctggcgccg
    13741 tggccgctgt aaaccagcgc ccccgatttc tcaccggccc aggcgctgag cgcctcggcg
    13801 gtttcacggt ttaatggcgc cgccaggcgg aaggggagat cgagacggcg cgcgtgcagg
    13861 gctgtgaagg ccagagagct ggcatccgca ttctctctca gcgccccggc ataatccgtc
    13921 gccagccgcc gcaacgcgct gtcgctggcg gcgctgagca gcagcgcggt actttttctg
    13981 ccgctatccg tattggggag gcgcgcgttg agcgcgtcgg gcagcgaggc gacgatcata
    14041 tggcagttgg tgccgccaat accaaaggag gagacgcccg catagcgcat ttcgtcctgc
    14101 catgcctgtg ccgacaccgg tatggtaaag gggctctctt caagtttcag cgccgggttg
    14161 ggggtgtgaa aattcagtaa gggaggaatt tgcccgcgac tgactgccag aacggttttc
    14221 agcagtccgg caatgcccgc cgcggtatcc agatggccca tgttactttt cacggaaccg
    14281 agcgcacagc gctgatcctg cgggcgaggc gcatagacgt tgcgtaacgc ttcaatttca
    14341 atcgcgtcgc ccagcggtgt gccggtgccg tgggtttcaa tgtaacctac ctgcctgtcg
    14401 tcgatggccg ccagcattaa cgcctcttcg atgactgcct gttgccctgc gacggaaggg
    14461 gcggtatagc cgacctttct gttgccgtcg ttgttgaccg cgctggagag gatcaccgag
    14521 ataatcggat cgcctgacag cagcgcgtct ctcaggcgac gcagcaccac gcagccgaga
    14581 ccgttaccgg cccaggtgcc ctcagccgag gcgtcaaagg gacgacagtg accatcagga
    14641 gagaaaatca ttccgggctg gtagcggtag cctgcctgct gggggaaaga gagcgccacg
    14701 ccgccggcaa ccgccatatc ggattcgcca gcgcgcaggc tttcacaggc cagatgcacg
    14761 gcaaccagcg agctggagca ggcggtctgt accgataacg ccgggccgtg caggttgagt
    14821 ttgtacgcgg cgcgggtggc aatatagtct ttatcattgc ccatcagaga ttgcagacct
    14881 tttacctgcg cgacttctgt cacgttcaat gcttcgcgac cggggtaggt actcatccgg
    14941 gaagaggcga aaacgccggt cttatggggg acggcgccgg gggcataacc ggcatgttcc
    15001 agcgcatgcc agaccgcctg caaaaacagg cgctgctgcg ggtccatcga ctccgcttcc
    15061 tgtcgcgaat agccaaacag ggtggcgtcg aagcagtcgg cgttgtctaa caccgaaccg
    15121 atattgacat aatgaggatc gtcaatgatg gcggcatcca gaccgacggc cagaagctct
    15181 tcgcgcgtaa agcgtcggct gcattcacga ccttccagca gattttgcca gaacgtttcg
    15241 ccatccggcg attcaggaaa atggcaggcg tagccgataa ccgcgacagg ttcgcagtcc
    15301 gggtagtgtt gagcgatcga tgcatcaatg gaatctgctg tcggcgcaga agagaagcgc
    15361 aagttatcca taatcggtcc cgtttcctaa gattcacaaa gtcaatggaa cgccatgcgt
    15421 tcgcgatggc gttccgggga aaatcagttt gcttcgcgct atatccgccg ctgacgacgg
    15481 cgaacagggc gaggtcgttg tgttttctcc accgtggcga cgccggccag ataatcggcc
    15541 agggcagcag gagaagggtg ggtgaagagg tcaagcaacg tcagggtgga aaattcgtga
    15601 cgttgtaaca aaacgtgcag ttgcaccaga ttcagcgacg ttgcgcccgc ctcaaagaag
    15661 ttttctgcgg gcgtgacaga ctcgccaacc acctcgcgga aggcgtcgca aatcaggctg
    15721 acggtgctct cgtccgccgc aatgtgtggc tgagagtgct cagggcatac gcgcggtgag
    15781 cgctctgcgc attgccctgc gtgtttaacg ggaggcacca gcgcggcgag cggcagttgc
    15841 cagccgcttt catcctcggc cattcggttt agcaatgcgc aatactgttc aaactggcgc
    15901 tccacctgcc cggcaggaaa cagcgcggcg acaaaatccc agttaaagcg cagttcgcct
    15961 tcggattcat aaatctggtg atcgagccag acctgcggcg tctgggagat gccccagacc
    16021 ggtttgagca gattacgccg ggcgaggaag ttatcctgct caaagcccag cgcgctggta
    16081 aagacgacgg gcatcggaac ggctggcacg ttttgccgtt gcgccagttg acgcatcacg
    16141 cggatggctg acacatcgcg gtggttgagg ttctggctca gccgctgctg tagtgactgc
    16201 gcgctgtgca gccagctttc gccgggatgc cagctcagca gcatcagcga ggtgaaatcg
    16261 cccagaatct ggttgatttg cgggtgcagc ggtcgcctgt cgaaaagcgt aaggttaagc
    16321 gtgaagtcag gctgtgcact ccatgcagag agaaccgttg accacaccga caacagtacg
    16381 gccgacgggg tgagatgggc gtcagccgcc cgttttttca gccgatgcca gcgcgtgctg
    16441 tccagtgcgc cgttcaggcg ggcgaagcgc ggtgtttcaa cctcctgagg caagcagcgc
    16501 agcggcaacg caggcgccgg aggaatatca tcaagctgcg cctgccacca tgccagagaa
    16561 tctggattgg gcgactgtag cgagggttgt tgcagataat ccctgaaggt gacgggcagc
    16621 ggcggaagca gctgttgcgg gtagcggtag ccgtgctcca gctccgccag caggatctgc
    16681 atgctcagac cgtcaagcaa caggttatcc agacacagcc acaggcgggc aggcatcccg
    16741 tccacgtatc cgacctggag atcgaatacc ggccatactt cggggttgag tacctgatgc
    16801 gccagttttt cgcgcacccg caacgcctct tcaggcgtat ggagggtgtg tgcgggtatc
    16861 acccaggggg gcgtctgttc gagcacctgt tgctgtccat cacgcacgat ggcgcgcagc
    16921 atatcgtggc gggcgattaa tcggttccag accgtctcca gccgggtgag gtccagatcg
    16981 gcaatttcaa attcaacaaa gaaatgtgag ccgacgccgc ccagggcaaa gcccggctga
    17041 cgccccacca ggtaagcctg ctgcacgtcg gtaagcgcaa agggctggta gcgatcttca
    17101 ggggagtgga cgaatggttg ttcgaccggg acgtcggttt tccgcagcgt ggcggcaaaa
    17161 tccgccagcc ggggatgatt aaacaggtcg cttaattgcg cttcataacc tgcctgatga
    17221 agttgcccgg tcagacgggt cgccagcagg ctatcgccgc cttgctggaa gaagtcggtt
    17281 tctctggtga cattgccagt tgataagagt tgctgccaga gggcggcaac ctgtttttca
    17341 atgtcgccct ggggtaaatc cgcttcggcc gggttttccg cttcaggggt atgacgacgc
    17401 ttcagcgcct ggtagtcaat tttcccgtta gctgtcagcg gtaaggcgtc gaggaagacg
    17461 atccgttgcg gcaccatcca ccccggcaga cgcccggcga gggcggcctg aagttgacgg
    17521 ggatcgcggc gcacctgcct gtcaggacat tgtacgagga agcgttgtaa ccccgcgacc
    17581 tcatcgccat gctgacagtt gaagccggcc gcgctaaata atgccgccca gtcggcgctg
    17641 ttatgcagca aggcctccgg ctgcccattg gttaacagga gcgtgctgag tagggcggac
    17701 ggcgttaact ggcgaaactc catcacgtag agcagcgcgc cgggaacggc aagctgttgt
    17761 aatgtcgcaa ggagcccggg atcttccggc agcagacgat gcagggcgtt attaagccag
    17821 ataatgtccg ccgagtgagc gtgcgccgcc agcgtgtctg cattccagag ggacagacgg
    17881 gcgccaggcc agggggcgag cctctgccgg gcgctcagca gcatctcctg gctctgctca
    17941 agcccgacat actcaatctg tccggcgttg agctgtgcta acagcgattc tgcggcgcgg
    18001 ccagtgcggg ttcccacctc aagcaggcgt accgggcgct gtaagcgctg tgcaagctgt
    18061 tggcaaattg tcgccagttc ctgaatatac ggggcgctgg ccggatggtt aaaggccagg
    18121 ctttccggcg accactgcgc gtcgttcagt aacgacagtt cgttacgggc accgcgaaga
    18181 atttgcgata acgttaaatc atgtccgctg aagtgcgggt gctcatcctc gcgttcttca
    18241 ccagcgcaga cctgataacc ttcagcagcg ggcttcagtc gccgttgtgt caccagaaat
    18301 gctaaccagc gttccaccac ggcctgccag cgcggctgga tagcgagtga gttcatcagg
    18361 gggagaggat cggcgccagc ggtgtgaccc ggcttcagtt ttagcaggcg atgctgaagg
    18421 aaatcggcta cctgttctgc ggagatctct ggcgagatgg cgcaacaggg caacgttccc
    18481 gcaagcgtgt gccacgcctg cggcagtgcc gggttccgat gatcggtaac gcaaaaagcc
    18541 tcgccctgag gaacaacgta tgccgccagc gttttttctt tttcgccgat cgccagaacg
    18601 gttgcttgtt tcacccccac cagctggctg agcgcgcttt cgatttcgcc cagctcgatg
    18661 cgatatcctc cgactttgac ctgcttgtcg cgacgaccga ggaactcgat tgtgccatct
    18721 ggccagtagc agccgagatc gccggtgcga taccagcgct cgtccgggag cgtcaaaaat
    18781 tgctgctcgc tacgcagggg atcgttgaaa tagccttccg cgaccccaat gccgccaatc
    18841 cataattcac ccggcaccca gtcagggcag tcccggccct gttcatccac cacccggtag
    18901 cgttggttgg ttagcggaaa accgtaaggg atggagcgcc agtaggcggg gacgtcgtga
    18961 atttcgcagg cgttagacca gatagacgcc tcggtggcgc cgcccatcgc gataaattgt
    19021 ccttgtggcc ggaaggcccg ataacgggcg gggaggtcaa gcccgatcca gtcgccggaa
    19081 agcatcactg cgcgcaggtt ttccggcgtg gcgtcggcga aaccttcaca ccaggtcagc
    19141 agcatatcga acagcgccgg gacgctgttc cagagcgtga cctgatggcg ctggatcagc
    19201 tcacaccatg cgtgaggatc gcgccgttga ttttccatca ccatcaccag cgcgccgccc
    19261 gcgcgcagta cgccaaaaat gtcgtaaacc gataaatcaa aatgtagggc ggagagggcc
    19321 agcaccctgt catgcgggcc aacctgatag cgggtattga tatcgcaaca ggtgttaagc
    19381 gctccccggt gagaaatgac tacccctttc ggcgtaccgg tagagccgga ggtgtagata
    19441 atgtaggccg gttgcgtggg ggcgcgtact accgggttgg cgatcggctc cgcctcaatg
    19501 gcctgctgcc aggcaagggc gggaatatcg tctgacccgg cgctggcgtc gtgctgacaa
    19561 atgagcacca gccggacgct ggcgtcagcg tagattttct cgcgccgtgc ggcaggctga
    19621 tccagcgaaa ccggaacgta aaccgccccg gccagcagga cggccagaac cgcaacaagt
    19681 tgtcctgcgc ctttcgacat cgtgatagcc acattatcgc cgggctgaac cccgcactcg
    19741 attaacctgc ccgcgcaacg gcgggcatag tctgtcagct catgataatt ccactgataa
    19801 cgcatgtccg ttaccgccag cgcctgcggc tgttgcagag cgatacggaa aatgccttca
    19861 tgcagcaagc cttcgggaat gggggcgccg gtggcgttga cccgttcgcg tatcgcgcgc
    19921 tggctggcgg gcatcatatc tgcgaacggc ttttgccagg cgctttcgtc atcgcagagt
    19981 tggttaatca actggcagta ggcgtcgaac aatgtttcga ctaacgccgg agggaacagc
    20041 gcgtcgttgc tgtcccattg tagccagacc tcgccgtgat gctcgaacgc cagatgatct
    20101 atccagacct gcggcgtttg cgagatgccc cattccggct cgcccaacgg cgattctgcg
    20161 cggctgctgt agagggaacg ccccagattg ctggtaaata ccaccggggc gccgtggggg
    20221 tagcgctgct ggcgtttgag ttcacggagt aattcgacgc cggaccagtg gcgatgctcc
    20281 cagtcctccg taaacgtgag ctggttttta cgcgccaggt tgctgacggt atcgccatcg
    20341 caggcggtat ccagcagaag aatattggtg aagtcggcaa gcatcgcgcc aaccgccggt
    20401 tgcagcggct ggcggtcgaa taaggtgatg ttaagcagca gacgcgtcag gccgccccag
    20461 cgagccagca cggcagaaaa acaggtcgcc agtgccattg tcggcgtcac gccatactcg
    20521 ccggcccggt tgctaaaggc gtgccagcgt gttgccggga caatcatgcg gcgtcgggta
    20581 ttacggactt cacgtagcgt ggcgggttcg caggccagcg gcaagacggg cgcgggggga
    20641 agcgtcgatg ctttcgccag ccagtaagcg cgcgcgacgt ctctcagtgg ttgattgatc
    20701 ttctgctggt gcagcaaata cgagcggaaa tcatagcggg tgtcgatagc cggcagcgat
    20761 tctccggcca gcagggcgtt aagctcatcg aagaaaagcg taaagctgga ggcatccata
    20821 atcagcaggt caatattgac atggaggcgg tggcgattgt ccggcaagag cgtcagctga
    20881 aaatcaaacg tttcgccgat ttccacgcgt aaaagacggt ggctcaggcg ctggcgcagt
    20941 gcgtccagat aggcctggcg gctttcagcg tcgttatggc gtaaatcatg aacggtgacg
    21001 ccgttccagt aaggttgcgg tagccagacc tgctgcccgt cggggcgaaa ggcgatatgc
    21061 agcattgggt ggcgttgcag caaggtcgtg atggcctgct ccagctgcga cgccgtcaga
    21121 caatggcctt caaactcctg atacaggtgg caacccacgc cgccaagcgt ctgccccggc
    21181 atgcggcccg tcaggtaggc gtgctgtact ggcgtcaatg ggaagggggt actttcggtc
    21241 atgttcggcc aggatgattc gtcgggcggc gtttcttctt ccgcgttctc cggcgaccgg
    21301 ctgagcatta actggttcca tgccgccagc gtgggggcgg catacagctc gcgaagggta
    21361 aggcggtagc catttttacg aaaccagtgt aaccatctca tcaatcttat ggaatccaga
    21421 ccggcctgga tcaggttgct ctcttcatgt aactgctgcg gcgttaaatt cagttcctgt
    21481 atgagccgct cgcgtaattg ttggtaatca gccgcgtggc ggttgtccgg taacagcgaa
    21541 tcctgagatg gtgcgccaga aatcattctt cctcctgatg gcacgtctta ccgaaaagca
    21601 ttactcatat gagaataata attattaaca attggatgct acccgaaacg ggtttattta
    21661 tgcccgtttc gggttggtaa caggcggaaa gaacagaggc ccggatgggg tatccgggcc
    21721 tctgtcaggg aggagtttag gggggcgcga ccccggttac atcccgcgtt taaaggtcga
    21781 aggagttacg ccaaactgtt tctggaaggc ggcagagaaa tggctggcgt tggcatagcc
    21841 cagatcggcc gccaccgtca tgacggaggt attgccgttg gccaggcgtc ttctggcttc
    21901 ctgcattcgt tcagcctgaa acaggccata gacgctgttg ttgaacagaa cgcggaagcc
    21961 tcgcttaatt ttgagaacgc tcagaccgga ctctctggcc agctccgcga cgcccggcgc
    22021 ttgcgtcaga tcagccagca gcagatcctt cgcccgcagc agtttctggc gttccatcgg
    22081 gttgaagtgg caggagaggc aactggcgtc ttcgcgctgt tcatccagca ccagactgag
    22141 catgaccaga ctttgcccct gcaaccatag cgtcgacggc ttttgttcat gattgaagcc
    22201 gtcgtgcatg cgcatcagcg cgtgacgcaa ggcctgagcc gttaagtggg tttcagcatt
    22261 acaacgctgc tgctggcaac agcagtggga gtcgatctta ttcttaatga ccgcgctaat
    22321 atccggcacc cacagcgcga gggtttcagg gctgaaggag agggtaatgc tctcgaatcg
    22381 gccacaatag gaggcgctgc cgcgacagtc tggcgtatgg gtaatgtaat ggcgattgcg
    22441 cgcaagctcc cagtcggtgt ggcgccggag tccctgaatc gcaaagcgcg atgtgccttc
    22501 caggatgcac gagaagttaa tcctgcccag atcgtcgtgt agctgcaacg tctcaggccg
    22561 cgcaaaatgg cccctccaga gcaaaatatc cataccttcc tgcaacgaac agcgcagcag
    22621 ttcacactga gcaggaatat tgccatcgtt tgcaggtttc ccgaccacca actggtgaat
    22681 agagatttca gattgcgttt gcggtgactc cgtcatgacc tggttatctc cctgtggctt
    22741 agaccaatgc gggtataaat agaaccgaat ggggtagatg agttattgat aatggttatt
    22801 attcacatta attctcagaa cgccaccctg aattgtcgtt tttggcgtcc gtaaaaatgc
    22861 atcaggaaat tcagttactc ccatgtcatc tcaatcatcc aatactgaat cgctgtcccg
    22921 atttcctctc tggcaggtga ttacaccggt tcgcaggaag gttattttag ctatggcgct
    22981 ggcggggctg gcggcattaa ccagcctggg ggcgctcctt tttctggtgt ggagcctgcg
    23041 tgacattcgc gcgacgcctg acgctattcc ggcctggccg ctgggtggcg tgatcggctg
    23101 cgtggtctta acttttgttc tgcgcttaca ggcgttcaac acctctcatt acgcggcttt
    23161 tcatctggag aacattctgc gcagcaggtt agcccgtaaa gcattgcagc ttccgcctgg
    23221 cgtgttacag caaatgggca gcgggtcagt ggcgaaagtg atgctggatg acgtgaagtc
    23281 gttacatatt tttgtggccg acagcacgcc gctctatgcc cgcgcgatca tcatgccgct
    23341 ggcgacaatc gttatcttgt tctggctgga ctggcggctg gcaatcgcga cgctgggggt
    23401 actggcgttt ggatcggttg ttctcgtgct cgcccgccag cgttcggaag atatggctca
    23461 gcgttatcat aaggcgcgcg agcaggttag cgcggcggtt attgagttcg tgcaggccat
    23521 gcccgtggtg agaacgtttg atagcggcag caccagtttt ttgcgctatc aacgcgccct
    23581 tgaagagtgg gtcgatgtgc tccaaacctg gtatcgcaaa gccggttttt cagcgcgttt
    23641 ttccttctcg attctgaatc ctctcccgac cctgtttgtc ctgatctggt cgggatacgg
    23701 cctgttgcac tatggcagtt tcgattttat cgcgtgggtg gccgttttac tgctgggcag
    23761 cggaatggcc gaagccgtaa tgccaatgat gatgctcaat aacctggtcg cgcaaacgcg
    23821 tttaagcatt cagcgtattt atcaggttct cgcgatgccg gagttatcgc tgccgcagtc
    23881 tgagcagcag ccgcaagagg cgagcattac ctttgagcag gtgagctttc attatccgca
    23941 agcgcgtact ggcgccgcgt tgcaggaggt gagctttcat gtgcctgccg ggcaaattgt
    24001 ggcgctggtc gggccaagcg gcgccggaaa aagcaccgtg gcgcgcttgc tgctgcgtta
    24061 cgccgacccg gacaaaggcc atatccgtat tggcggcgtg gatctgcgtg atatgcagac
    24121 ggacaccctg atgaagcaac tctcgtttgt gtttcaggac aacttccttt ttgtcgacac
    24181 gatagccaat aacattcgtc tgggcgcgcc ggatacgccg ctggaggcgg taatagcggc
    24241 ggccagagtg gcgcaggccc atgattttat tagcgctctg ccagaaggtt acaacactcg
    24301 agtcggggaa cgtggggtat ttctctccgg cggccagcgg cagcgcatta ctatcgcccg
    24361 ggcgcttttg caggatcgcc ccatcctggt gctcgatgag gcgacggcgt ttgctgaccc
    24421 ggaaaacgaa gcggcgctta tcaaggcgct cgcggcggcc atgcgtggcc ggacggtcat
    24481 catggtcgcg catcgtctct caatggtgac tcaggccgat gtgatattgc tgttttccga
    24541 cggacagctc agggaaatgg ggaaccatac gcaactgttg gcgcagggcg gcctgtatca
    24601 acggctctgg caacactatc agcaggcgca gcattgggtg ccgggtggaa cacaggaaga
    24661 ggtggtggaa aatgaaagac aataatcctg cggataacct ggcctggcgc gtcatctggc
    24721 gccagcttat ctccagcgtt ggcagtcagg ccaggatgct gcgacgcagt atgctggcgc
    24781 tgttgctggc ggcattcatg caggggatcg cctttgcctg tctttatccg atcattgatg
    24841 cgctgttacg gggagacgcg ccgcaacttc ttaactgggc tatggccttc agcgtcgccg
    24901 caattgtgac gctggtgcta cgctggtatg gcctgggctt tgaataccgt ggtcatctgg
    24961 cgcaggctac ccatgagttg cgcctgcgac ttggcgagca gttacgccgc gtgccgctgg
    25021 agaagctcca gcgcggcagg gcgggtgaaa tgaacgcctt gctgctgggc agcgtggatg
    25081 aaaacctcaa ttatgttatt gcgatagcca atattttgct gctcaccatt gtcacgccgc
    25141 tgacggcgtc gctggcgaca ttgtggatag actggcggct ggggctggtg atgttgctga
    25201 tcttccctct gctggtgccg ttttattact ggcgccgccc ggcgatgcgg cgacaaatgc
    25261 agacgctggg ggaagcgcac cagcgtctga gcggcgatat cgttgaattt gctcagggga
    25321 tgatggtatt gcgcacctgc ggcagcgatg ccgataaaag ccgggcgctg ctggcgcatt
    25381 tcaatgcgct ggaaaactta cagacccgca ctcaccgtca gggcgctggc gcgacgatgc
    25441 tgatcgccag cgtcgtggag ttgggcctac aggtggtggt gttatccggg atcgtctggg
    25501 tagtgacggg cactctgaac ctcgcctttt tgattgccgc cgtcgcgatg attatgcgct
    25561 tcgcagaacc gatggcgatg tttatcagct acacctcggt tgtggaactg atcgccagcg
    25621 ccctgcaacg gattgagcag tttatggcga tagcaccgct tcccgtcgca gagcaaagcg
    25681 agatgccgga acgttacgat atccgctttg acaacgtcag ctatcgctac gaagaaggcg
    25741 acggccacgc gcttaatcat gtttctttga cgttcccggc agccagtatg agcgcgctgg
    25801 tgggtgcctc cggcgcaggc aaaactaccg tcaccaaact gttaatgcgc tatgccgatc
    25861 cgcagcaagg gcagatttct attggcggcg tcgatattcg ccgcctgacg ccggaacagc
    25921 tcaatagcct gatttctgtt gttttccagg atgtctggct gtttgatgac acgctgctgg
    25981 cgaatatccg tatcgcgtgc ccacaggcga cgcggcagga ggtagaagag gccgcccgcg
    26041 cggcgcagtg ccttgagttt atttcccgtc ttccgcaagg ctggctgacg ccaatgggtg
    26101 agatgggcgg ccagctatcg ggcggcgagc gccagcggat ttccattgcc agagcgttat
    26161 tgaaaaacgc gccagtcgtc attctcgatg aaccgactgc cgcgctggat attgaaagcg
    26221 agctggcggt gcaaaaagcg atcgataacc tggtttacaa ccggacggtg attatcatcg
    26281 ctcaccgttt atccaccatc gccggggccg gaaacattct ggtgatggaa gagggacagg
    26341 tggttgagca gggtactcat gcgcaattgc tctcacatca tgggcgttat caggcgctgt
    26401 ggcaggcgca aatggccgcg cgcgtgtggc gcgacgacgg ggtttccgcg tctggagagt
    26461 gggtgcatga gtgatgttca gtcgaatgtg aaaccgctga cgttgacgac cgggcgggtg
    26521 atttttgcta ttgccggcgt ctatgtgacg cagagtctgg tatcggcgct gtctatgcag
    26581 tccttacccg cgctggtgcg cgctgctggc ggttcgctgg cgcttgccgg tgcgacaacc
    26641 ctgtttatgc tgccctgggc gctgaagttt atttgggcgc cgtggatcga gcgctggcgg
    26701 cttccgcccg gtagccagga acgccgttcc cgcatgttaa tcctgcgtgg tcaggtcgcg
    26761 ctagcggcga tcctgactat tgccgcagcg attggctggt ttaggcgaga agggggattc
    26821 cccgatacgc aaatcgtcgc gttatttgtt ctgtttatgg tggcaggcac ggtcgcctcc
    26881 accattgata tcgccagcga cggcttttgt gtcgatcaac tgacccgcac gggttacggc
    26941 tggggaaaca gcgtgcaggt cggcggcagc tatctgggaa tgatgtgcgg cggcggggtg
    27001 ttcatgatgc tgtcggcggc atccggctgg cctgtcgcca tgctgatgat ggcggtgctg
    27061 attatggcgc tgtcactccc gctgtggcgc attacggagc cgacgcgaac agcgactatc
    27121 ctgcatgttc cggcgttagg ttatgcgcta aggaggaagc aggcgcgcct gggcttactg
    27181 ctggtattga tgctgaattc aggcatgcgg tttgtgctgc ctcttctggc accgctgttg
    27241 ttggatcatg ggttgagcat gtccgcattg ggcgcgttgt tcagcggcgg caatattgca
    27301 gcgggcatag caggaacgct ggctggcgga ttgctgatga aatacacctc acccggcaga
    27361 gcgctgttga cggcttatgg cgtccagggg atcgcgctgc tggcggtggt gatgacgctc
    27421 atgatggcgc cgggtcatct gctgctgcag attctccagt gtctggtcat tgtccagtcc
    27481 atttcgctgg cctgcgcgct ggtctgtctt tacgccacgc tgatgtcgct ttcatcgcct
    27541 ttgcaggccg gtgtcgactt caccctcttt caatgtactg atgcggcaat cgccattctg
    27601 gcaggcgtta tcggtggcgt tgttgctcaa cattttggct atgcggcctg cttcctgttt
    27661 gccggggcat tcacgttgct ggcggcgtgg gttgcttata tccggctgca ttcggcaaga
    27721 gaactgatga caagcgcaat tgattgatcc gcgaataaca cagagaggta agggatgaaa
    27781 atcagtgaat ttctacatct ggcgttacca gaggaacaat ggctaccgac gatttctggc
    27841 gttttacgcc agttcgcaga agaagagtgt tatgtctatg agcgtcaacc ctgttggtat
    27901 ttaggcaaag ggtgccaggc acggctgcac attaatgccg acggaacgca ggcgacattt
    27961 attgatgatg ccggggagca aaaatgggcg gtggattcca ttgccgactg cgcgcgtcgt
    28021 tttatggcgc atcctcaggt gaaaggacgt cgggtatatg gacaggttgg gttcaacttt
    28081 gcggcgcatg cgcgggggat tgcctttaac gccggggagt ggccgctgct gacgttaacc
    28141 gttccccgtg aagaacttat ttttgaaaag ggaaatgtca ccgtttatgc ggactccgcc
    28201 gacgggtgcc gacgtttgtg cgagtgggta aaagaggccg gtacaacgac gcagaacgca
    28261 ccactggcgg tggataccgc cctcaatggt gaggcatata aacaacaggt tgcgcgcgcc
    28321 gttgcggaga tccgccgtgg cgagtatgtc aaagtgattg tctcgcgcgc cattcccctg
    28381 ccatcgcgga ttgatatgcc cgccacgctg ttatacgggc ggcaggcaaa cacgcctgtg
    28441 cgctcgttta tgttccgtca ggaaggacgc gaagcgctgg gctttagccc ggaactggtg
    28501 atgtcagtga tgggcaataa agtggtcact gaaccgcttg cgggcacccg cgatcgcatg
    28561 ggaaacccgg agcataataa ggcgaaagag gcagaactgc tgcacgacag taaagaggtg
    28621 cttgagcata tcctttctgt caaagaagct attgctgaac tggaggctgt ttgccagccg
    28681 ggcagcgtgg tggttgaaga tttaatgtcg gttcgccagc gcggcagcgt tcagcatctg
    28741 gggtctggcg tgagcggtca gctttcggaa aacaaggatg cctgggatgc gtttaccgtg
    28801 ctgtttccgt cgattaccgc ctcaggtatc cctaaaaatg ctgctctgaa cgcgattatg
    28861 caaattgaga agacgccgcg agagctttat tccggcgcaa tcctgctgct ggacgatacg
    28921 cgctttgatg cggcgctagt tctgcgttcc gtatttcagg atagccagcg ctgctggata
    28981 caggcggggg cgggaatcat cgcgcaatct acgccggaac gcgaactgac agaaacccgg
    29041 gagaaattag cgagcattgc gccctattta atggtgtagg agtaactgtt ttctaacctc
    29101 acaaccagac tgtccaaaga caattgagtc gctctttgat tcctctgtga ctctattgtc
    29161 ttcttttggg ataaaatagg tgtttttcgt catgaagttg ataaaagtct atatagaacc
    29221 acctcctgtg gaggtggtgg taatttgcca gagtgaggga ttcgttagta tactatggga
    29281 taaacttatt tttaccaatt gtataaggcg gcgcataaga ttctcggcat gcttccagat
    29341 aatccgacca ccactgcatc atcgctttgc gggcttctag gtgctctgcc ttatgaatat
    29401 aagccatgcg cacggtattg cgctcctgat gactcatctg gcgttctact gcgtcctttg
    29461 cccataaacc cgattccatc agcgcactgc atgccattgc ccggaagccg tgaccgcaga
    29521 tatccttttt cgtgtcgtaa cccatcaccc gcagtgcctt attgaccgtg ttttcacaca
    29581 ttggcttata cgggttatgg tcgccgggga agatcagttc attattaccg gtgatatcct
    29641 taatctgttt cagaatggcg atggactgtt ctgagagggg gacgatatgc ggcattcgca
    29701 tttttgcccc gcggccggaa taacgcacgc caataatggg ttctcgcgtc gcgggtatcg
    29761 tccagactcg gtttgtgaaa tcaatctctg accagcgggc gaaacgcagt tcactggagc
    29821 gaatgaacac atgcagcatc agcagaacgg catgccgggt cagttcacgg ccctgatgat
    29881 atgccccaat acgttcaagc agttcaggca gccgctccag cggcagggca ggatagtgcc
    29941 gtctgacagg aggtgtggtc acgccgccaa ggtttgctgc agggttcgta tcgattaact
    30001 cctgatggac cgcatggcgc attatgttac tcaggtgctg ccgtgtgcgg gacgcaacct
    30061 ccagctttga gataccagtg gcgggagcca cccggcttga ccagcagata cagaccgtga
    30121 gaatcggaga ctttaaaggg tttatcagaa ggcttgaggg tgcggatttt tgcgtcggta
    30181 aggaacatat gggggtcact ccattatcga actaacctga tcccagattt gaccaccaat
    30241 ttttcccgat gcatagggta aaatcaaaat gcaccgagaa gagttttcac actaacctgt
    30301 tgaatcaaca tcagataaag atttgcaagg atgcatgaaa acagaaaatt ggctcctctg
    30361 actggactcg aaccagtgac atacggatta acagtccgcc gttctaccga ctgaactaca
    30421 gaggaatcgt gtgaacggag cgcatattac ttagcggtac cttgtctgtc aacactaaat
    30481 ttcataagcc atttcaattg gttaattaat ctgcaaagtt gttaattaat gaacattcgt
    30541 cgccgaaaac gagtctgtat cattagcgtg atgcagtctc tgcaaaggat cttgttgata
    30601 aaattgacag aaacgttgcc acaatgaagg gaaacgagga gcaaaaagtt ctggggcgct
    30661 aaagaaatat tcagaaagta cggcaaaaca ttcagcagga tcactggcag cataagcatc
    30721 aatgctcgct gcattctcac caactaattc gatttcttcc tgaatgttgt tcattgcagc
    30781 atgaagatcg tgttcccagc cagcaacctc acgcaacgaa ataaagggaa ctccgctggc
    30841 gcgatcgccg ttacgggtgt ccagcttatg agcgacttca tgaataatca ggttaaaacc
    30901 agatgcatca aaagaatctt gtatatccaa ccagttcaaa acgataggcc cttgctgcca
    30961 gctctgacct gactgaacaa tacgttggtt atgcaccaga ccgatatcgt cttcccattc
    31021 atcatcgacc acaaatggcg caggataaat taagacttca tgaaaaccat ccagccattc
    31081 cagtcctaac tccagaacgg gtaggcaaaa tagaagtgct atccggcagc ttcttaatga
    31141 atccagttca aagccctgta aaggaacaag ccgcttttgc tgtaaaaaac gttcggcaag
    31201 agtgactaat ttgctttgtt cctgttccgt cagacccgtt aaaaggggga tcgatagtgc
    31261 ttcctgccag ggaagggcag tttgatgtgc tgattcttgt actttccagg gccacttaat
    31321 catcgttttg ctcgcaaact cgtcacttga acaaaattgc acggacaggg actgttaaaa
    31381 tgccaaattt cctggcatca tggcaaccat ctgaacggag agatgccgga gcggctgaac
    31441 ggaccggtct cgaaaaccgg agtaggggca actctaccgg gggttcaaat ccccctctct
    31501 ccgccacttt atcaatgact tatctcctga cttcccgcct tgcttttcct aaacagaaca
    31561 atcgtagaat attcttgaag ggttagatcg tcactgtttt ctgttcgata ctgtgacatt
    31621 cagcacttga ttcgctatgg agctgacagg aaggtttcga gcgaaaatct acagttattc
    31681 agtcgttttc ttatcggtca ccattattct tttagacatt gatcctatga aactgccgca
    31741 aagttgctgg cgggaactga agttgcgtag agaaggggtc aataattgag tcaaaaaagg
    31801 aacgcggccg tcagattaaa gccgcgggac aaagtccata atttccaaga gttgttgaat
    31861 ttcccagctt ttttgaaagc ctttgtatca tactaacaag taaaaaaggt ttaaacacca
    31921 aaattaacct atattactgg ttagctttac catgacagat gacttgctcc tggaaacatt
    31981 tgcgctgcca gtctgtatgg ctgagttgtg tatttattgt tcgccttttt cgcatttttt
    32041 tctacagaca taataaaaaa gacacgactc aagtatgagc gtgtctgagt ttgtcttact
    32101 tatttgtctt ggcaaattat tgttgttact accaggcact gttctgttaa ataactgaag
    32161 tcagcgcaaa aaaatcaatg agacatcatc tcctcgacca cttcttcgct actcaactga
    32221 tagggataat acgttggcca gttttccatt tcattcaata aagactgttg ggaatcatta
    32281 cccataaaaa tgtgaaaatg agatgatttt cgtggtgcaa ttatatgatc gctaaattgt
    32341 atatatttag gggctttact ttcaggatct ttacattcga ataagtaacg aacgcctttc
    32401 ttgcctgatt tataggtgag tattttgtat ccatcgtaat catatttaca ggatgttgtt
    32461 tcattatttc tatggaattc gacaatgccg tcctcaatgc caatcatctc gatgtctgtc
    32521 acataacctt tgcgataata atctttaatt tcagcaaatg ttttggtttt atctgcatcc
    32581 gctttcttct gaaagatggg gtcaagtttg ccactttgca gtaaaggata aacggattgc
    32641 cagactccat cccagtcact gagcgttcgg ttttgtacat tggcatcatc aaaaacacca
    32701 ttagcagctt tttgttcgac ctctgttaag ggtttgccgt gtgagtgatg accatgcgaa
    32761 aaggcaggag cgctaacaat aaagacacct aaagcaacag ccagtttatg aagacgaatc
    32821 gccaaaacag tttcctccag agttaatgta tatgatgtgt aatgttatat tgtaacatat
    32881 aacaaaatgc aacaactctt aggagacaag agaatagttc tctcaggtgt gcttatctat
    32941 cttattgaaa taaaataaat tatgtcgatg atttgaggtt gatgacaaac gaaacataac
    33001 ttgatgtgat ctgcgtaggc cggatagggc gctcgcgccg catccggcag ggataacgcg
    33061 tactttgtcg gcaatatgaa gttatacaga taatttgttg tgaacctgct tgcgtaaccg
    33121 gttaaaaagg gaaagcaact tcttataccg taaggctaaa agcagtaccg ccagcccagc
    33181 gtagatgagt ggctgcggtg aaataatctt cactgaccac aaataatgta ttggggccag
    33241 gatcgcgaca agatagacga agttatgcaa ctgttgccag tgtttaccga gttttcgctg
    33301 catcgcctgg gttgaagtga acgctaacgc aagcaaaatt acccagctga taatacctaa
    33361 cgttaaataa ggccgggtaa ttaactcctt acctaataac gccagattat tcacgcccag
    33421 ctccagcaat gcgtaactgg ttaaatgcag tgtcgcccag gcaaagcacc ataagcctaa
    33481 caggcggcga gtgcgtatca ataacggctg ttttgcgtag cgtgccagag gtgtgattaa
    33541 caaggtcgcc agcaaaaatt tcagtgcagt gcgaccagta aaatgctgaa tatctttcac
    33601 cggatcggca cccaatccac cgtgattaat cgcccagacc agccagagaa acggcaacaa
    33661 tccggcaaga tgcaggcaaa ctttcagcca tgtcacctgt tttgctgtca gacgcattta
    33721 gaaattctcc cgcaaatcca ggccacgata cagcgatgcc acctgctcgg cgtaaccatt
    33781 aaacagtagc gttggctggc gctgtacatc gaggatgccg cctgaaccaa taaatcgttc
    33841 ggtagcctgt gaccagcgcg ggtgatcaac atgcggatta acgttggcgt aaaaaccgta
    33901 ttcgtcaggc gctgccagat tccaggtggt tggcggacgc tcgcgggtca gcttaatact
    33961 gactatcgat ttaatccctt taaagccata tttccacggc acaatcagtc gcaccggcgc
    34021 gccattttgt ggcggtaacg ccttgccata aacacccacg gtcatcagtg tgagcggatg
    34081 cattgcttcg tcgagacgca atccttcgac ataaggatat ttcagcccgc caccgataaa
    34141 gcggtcctgc tggcctggca tttgttccgg tgcataaatt gtttcgaaag cgacatactt
    34201 cgcattgctg gtgggttcgg caagcgccag caatttgtgc agcggaaaac caatccacgg
    34261 caccaccatc gaccatgctt ccacgcagcg catacgataa atacgctctt ccagcggaaa
    34321 gcgacgggtt aaatcatcgt gatcgagggt caatggtttt gccacttcgc cgctgatttt
    34381 cagtgtccat ggatcggttt tcaggctacc agcattagcg gcgggatcgg ctttatccag
    34441 cccgaattca tagaagttgt tataaccgga gactttatct gctggcgtca gtggcaggtc
    34501 attttgccag gcggcaggct tgctgaactc caacggtttt ccggcggggg cgggtgggcg
    34561 atcattccct ttgaaccagc taagcagatc ggcatgcgca gcgtgaggca aagaaagtgc
    34621 agccgcgctg atgcccagtg cttttaacac ctgccgacgc gtcataaaga ataccgactc
    34681 ggccgtaaca tctgattctt ttaaaaattg attctttttc ataagccatc acactttttt
    34741 gtctttagcc agcaagagcc gtttactgtt aatctgtcag gaagtgtcct ccctaacagt
    34801 agcagagatc tgcttttaca tatttatgca ttaactgcca cgataggttg aatacccata
    34861 ttggctaagt aataaaggca catgataatg ctcgttcact ttattaatat gaaattcaac
    34921 ggggatctca gggaagaaac tttcaagatt ttgtttcttg aaatagtccc cggttttaaa
    34981 tacgacacgg taatcgcccg tagttgcagt ttgctcgggc cacagtgcct taattcgtcc
    35041 atccttatct gttttggcgg tattaagttg taaccagccg ttgtccgctt tcttttcaag
    35101 agtgactgtc acgtcggcag cgggttttcc tgtttgctgg ttcaaaatgt gcacactaag
    35161 aatgttttgt tgtgcagcat agaccaaaga aggtaatgaa aatgcagccg ttactacgga
    35221 gagtactaaa taacgcttta acatgtttat atccttgtca tgtgaatgag ttttgaggat
    35281 agcgaaaaag cgtccacggt aaccttacag cagcattaca attttgtaat gaaccaggct
    35341 gtttctataa catatgattt atggcatatt attttcatga agattttact tattgaagat
    35401 aatcaaagga cccaggagtg ggtaacgcag gggctttccg aagcgggtta tgtcattgat
    35461 gccgtttctg atggcagaga tgggctttat cttgcgctta aggatgatta tgcattgatc
    35521 attctggata ttatgcttcc gggtatggat ggctggcaga tcttacaaac gttaagaaca
    35581 gcaaagcaaa ctcctgttat ttgccttact gcaagagatt ctgtcgatga cagagtcaga
    35641 gggctggaca gtggggcaaa tgattatctg gtaaaacctt tttcattttc tgagttgctg
    35701 gcaagggttc gggcacaatt aaggcaacat cacgctttga attcaacatt agaaatcagt
    35761 ggcttaagaa tggactctgt tagtcaaagt gtgagcaggg acaatatcag tattacactg
    35821 acgcgcaagg agtttcagtt actttggcta ctggcctcca gagctggcga gattatcccc
    35881 agaacggtta ttgcgagtga aatttgggga atcaactttg atagtgatac caatacggtg
    35941 gacgtcgcca ttcgcaggct ccgcgcaaaa gttgatgatc cttttcctga aaagctaatc
    36001 gccacaatcc gggggatggg ctattcattc gtagcggtaa aaaaataatg aaaagattat
    36061 ctataaccgt ccgtttaacc ttgcttttta tattgctgct atctgttgct ggcgctggaa
    36121 ttgtatggac tctctataat ggcctggcaa gtgagttgaa atggcgcgat gatacaacac
    36181 tcattaaccg gacagcgcag atcaagcagt tgttaattga tggggtaaat ccagatacgt
    36241 tacctgtgta ctttaaccgg atgatggatg ttagtcagga tatcttgatc attcatggtg
    36301 atagcatcaa caaaattgtt aaccggacga atgtcagtga tgacatgtta aataacatac
    36361 ctgctactga gacaatcagc gcagctggca tttacagaag cattattaat gatacagaga
    36421 tagatgcttt acgaattaat attgatgaag tttcgccatc attaacggtt actgtggcta
    36481 aattggcttc agccagacat aacatgcttg aacagtataa aattaatagc attataattt
    36541 gcattgtcgc cattgtactt tgctcagtat taagtccgct gttaatcaga acgggattac
    36601 gagagatcaa aaagttgagt ggtgtaacgg aagcgctgaa ttataacgat agccgggagc
    36661 ctgttgaggt tagcgcatta ccgagagaac taaaacctct tgggcaggcg ttgaataaaa
    36721 tgcatcatgc tttagtcaaa gattttgagc gtctaagtca gtttgctgac gatctcgctc
    36781 atgaacttag aacgcccatt aatgcattac tgggtcagaa tcaggttacg ctcagtcaaa
    36841 ccagaagtat cgctgaatat caaaaaacaa ttgccggtaa cattgaagag ctggaaaata
    36901 tttcgcggtt aacagagaac atactgtttc ttgcccgggc agataaaaac aatgttttgg
    36961 tgaaactgga ctcacttttt ctcaataagg aagtcgaaaa tttgttggat tatcttgaat
    37021 acctttcaga cgagaaagag atttgcttta aggtcgagtg caatcagcaa atctttgcgg
    37081 ataaaatttt actgcaacga atgttatcga atcttattgt taatgccatt agatattcac
    37141 cagaaaaatc gcgcattcat ataaccagtt ttcttgatac caacggctat cttaatattg
    37201 atatcgccag tcctggaacg aaaattaatg agcctgaaaa actcttccgt agattttggc
    37261 ggggagataa ttcgcgtcat tccgaaggtc agggacttgg tctttcttta gtcaaagcga
    37321 ttgccgaatt gcatggggga agtgctacgt atcactattt taataagcat aatgtgttcc
    37381 gaattacgtt accgcaaaga aattaacata gggcttcagt acgccataaa tcaaccatga
    37441 aaaaacgcag ccacgtcgtg agtattaacg tggctgcgat tgattatgcg cttacattca
    37501 tacgtaacag ggattaaccc gcataagctg ccagcatttc ctgcgccgcc agtttaccca
    37561 acgcattcgc tgcgaaagga gtgtcgccgg tgagaacttt acggtcctta tgtactcgcc
    37621 cggtgatgtc gtcattaata atgttcatgc ccattttctt cagctcttcg ccgaagtacc
    37681 aggtgagatg acccggcata tagcctatct ctggcgtttg tttgtctgcg gcgtctggga
    37741 atgcgcaaat ggaataacca ttcagtgggt tatcgccgtg gcgaagcgcc agaaaagccg
    37801 ccgggccgtg gcaaagggag ataacaaaac ggtcattttt gattgcccac tgcaaagcag
    37861 cagccacgtc ctggctttct ggtaaaccaa taagtgcgcc atgaccacca ggaacaaaga
    37921 ttgctgcata ttcgctgtct gcgttgaggc tgacgacaac atccgcgatt tttttcggat
    37981 tgcggaacaa cgatttatgc tgctcaaaga atggcattac tttttcatct ttgtgcggca
    38041 tagcccagta ttcaaacttg gtcatcagac cggaaatggt cgccacttcg aattcgaaac
    38101 ctgcagcatg gagatgatac aacggcagca acgtttcaat cggatggtta ccggtcgaga
    38161 acagttttcc gttatcggtc ggcaaataac gttcgtccgc ggcgatcacc agaattttat
    38221 gtttaccgcg atacggtttt g
//
LOCUS       DAZFWL020000034        38054 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFWL020000034 DAZFWL020000000
VERSION     DAZFWL020000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 38054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.034"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            235..372
                     /locus_tag="ACHKNP_RS09145"
                     /old_locus_tag="pgaptmp_001829"
                     /pseudo
     CDS             235..372
                     /locus_tag="ACHKNP_RS09145"
                     /old_locus_tag="pgaptmp_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(480..1715)
                     /gene="aslB"
                     /locus_tag="ACHKNP_RS09150"
                     /old_locus_tag="pgaptmp_001830"
     CDS             complement(480..1715)
                     /gene="aslB"
                     /locus_tag="ACHKNP_RS09150"
                     /old_locus_tag="pgaptmp_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU4704011.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(1862..1938)
                     /locus_tag="ACHKNP_RS09155"
                     /old_locus_tag="pgaptmp_001831"
     tRNA            complement(1862..1938)
                     /locus_tag="ACHKNP_RS09155"
                     /old_locus_tag="pgaptmp_001831"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1902..1904),aa:Pro,seq:tgg)
     gene            complement(1981..2067)
                     /locus_tag="ACHKNP_RS09160"
                     /old_locus_tag="pgaptmp_001832"
     tRNA            complement(1981..2067)
                     /locus_tag="ACHKNP_RS09160"
                     /old_locus_tag="pgaptmp_001832"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2031..2033),aa:Leu,seq:cag)
     gene            complement(2088..2163)
                     /locus_tag="ACHKNP_RS09165"
                     /old_locus_tag="pgaptmp_001833"
     tRNA            complement(2088..2163)
                     /locus_tag="ACHKNP_RS09165"
                     /old_locus_tag="pgaptmp_001833"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2128..2130),aa:His,seq:gtg)
     gene            complement(2222..2298)
                     /locus_tag="ACHKNP_RS09170"
                     /old_locus_tag="pgaptmp_001834"
     tRNA            complement(2222..2298)
                     /locus_tag="ACHKNP_RS09170"
                     /old_locus_tag="pgaptmp_001834"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2262..2264),aa:Arg,seq:ccg)
     gene            complement(2401..3786)
                     /gene="yifK"
                     /locus_tag="ACHKNP_RS09175"
                     /old_locus_tag="pgaptmp_001835"
     CDS             complement(2401..3786)
                     /gene="yifK"
                     /locus_tag="ACHKNP_RS09175"
                     /old_locus_tag="pgaptmp_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4704012.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3977..4717)
                     /gene="wecG"
                     /locus_tag="ACHKNP_RS09180"
                     /old_locus_tag="pgaptmp_001836"
     CDS             complement(3977..4717)
                     /gene="wecG"
                     /locus_tag="ACHKNP_RS09180"
                     /old_locus_tag="pgaptmp_001836"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HGU4704013.1"
                     /translation="MNNNTMAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4720..6072)
                     /gene="wzyE"
                     /locus_tag="ACHKNP_RS09185"
                     /old_locus_tag="pgaptmp_001837"
     CDS             complement(4720..6072)
                     /gene="wzyE"
                     /locus_tag="ACHKNP_RS09185"
                     /old_locus_tag="pgaptmp_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HGU4704014.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6069..7148)
                     /gene="rffT"
                     /locus_tag="ACHKNP_RS09190"
                     /old_locus_tag="pgaptmp_001838"
                     /gene_synonym="wecF"
     CDS             complement(6069..7148)
                     /gene="rffT"
                     /locus_tag="ACHKNP_RS09190"
                     /old_locus_tag="pgaptmp_001838"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HGU4704015.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(7145..8395)
                     /gene="wzxE"
                     /locus_tag="ACHKNP_RS09195"
                     /old_locus_tag="pgaptmp_001839"
     CDS             complement(7145..8395)
                     /gene="wzxE"
                     /locus_tag="ACHKNP_RS09195"
                     /old_locus_tag="pgaptmp_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HGU4704016.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPATIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8397..9527)
                     /gene="rffA"
                     /locus_tag="ACHKNP_RS09200"
                     /old_locus_tag="pgaptmp_001840"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8397..9527)
                     /gene="rffA"
                     /locus_tag="ACHKNP_RS09200"
                     /old_locus_tag="pgaptmp_001840"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HGU4704017.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9532..10206)
                     /gene="rffC"
                     /locus_tag="ACHKNP_RS09205"
                     /old_locus_tag="pgaptmp_001841"
                     /gene_synonym="wecD"
     CDS             complement(9532..10206)
                     /gene="rffC"
                     /locus_tag="ACHKNP_RS09205"
                     /old_locus_tag="pgaptmp_001841"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HGU4704018.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10184..11065)
                     /gene="rfbA"
                     /locus_tag="ACHKNP_RS09210"
                     /old_locus_tag="pgaptmp_001842"
     CDS             complement(10184..11065)
                     /gene="rfbA"
                     /locus_tag="ACHKNP_RS09210"
                     /old_locus_tag="pgaptmp_001842"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGU4704019.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11084..12151)
                     /gene="rffG"
                     /locus_tag="ACHKNP_RS09215"
                     /old_locus_tag="pgaptmp_001843"
     CDS             complement(11084..12151)
                     /gene="rffG"
                     /locus_tag="ACHKNP_RS09215"
                     /old_locus_tag="pgaptmp_001843"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGU4704020.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12151..13413)
                     /gene="wecC"
                     /locus_tag="ACHKNP_RS09220"
                     /old_locus_tag="pgaptmp_001844"
     CDS             complement(12151..13413)
                     /gene="wecC"
                     /locus_tag="ACHKNP_RS09220"
                     /old_locus_tag="pgaptmp_001844"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HGU4704021.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13410..14540)
                     /gene="wecB"
                     /locus_tag="ACHKNP_RS09225"
                     /old_locus_tag="pgaptmp_001845"
     CDS             complement(13410..14540)
                     /gene="wecB"
                     /locus_tag="ACHKNP_RS09225"
                     /old_locus_tag="pgaptmp_001845"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGU4704022.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14596..15642)
                     /gene="wzzE"
                     /locus_tag="ACHKNP_RS09230"
                     /old_locus_tag="pgaptmp_001846"
     CDS             complement(14596..15642)
                     /gene="wzzE"
                     /locus_tag="ACHKNP_RS09230"
                     /old_locus_tag="pgaptmp_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HGU4704023.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNEELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15654..16757)
                     /gene="wecA"
                     /locus_tag="ACHKNP_RS09235"
                     /old_locus_tag="pgaptmp_001847"
     CDS             complement(15654..16757)
                     /gene="wecA"
                     /locus_tag="ACHKNP_RS09235"
                     /old_locus_tag="pgaptmp_001847"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HGU4704024.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16997..18256)
                     /gene="rho"
                     /locus_tag="ACHKNP_RS09240"
                     /old_locus_tag="pgaptmp_001848"
     CDS             complement(16997..18256)
                     /gene="rho"
                     /locus_tag="ACHKNP_RS09240"
                     /old_locus_tag="pgaptmp_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HGU4704025.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18341..18442)
                     /gene="rhoL"
                     /locus_tag="ACHKNP_RS09245"
                     /old_locus_tag="pgaptmp_001849"
     CDS             complement(18341..18442)
                     /gene="rhoL"
                     /locus_tag="ACHKNP_RS09245"
                     /old_locus_tag="pgaptmp_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HGU4704026.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18583..18912)
                     /gene="trxA"
                     /locus_tag="ACHKNP_RS09250"
                     /old_locus_tag="pgaptmp_001850"
     CDS             complement(18583..18912)
                     /gene="trxA"
                     /locus_tag="ACHKNP_RS09250"
                     /old_locus_tag="pgaptmp_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HGU4704027.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            19043..20308
                     /gene="rhlB"
                     /locus_tag="ACHKNP_RS09255"
                     /old_locus_tag="pgaptmp_001851"
     CDS             19043..20308
                     /gene="rhlB"
                     /locus_tag="ACHKNP_RS09255"
                     /old_locus_tag="pgaptmp_001851"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HGU4704028.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKYRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20442..21926
                     /gene="gppA"
                     /locus_tag="ACHKNP_RS09260"
                     /old_locus_tag="pgaptmp_001852"
     CDS             20442..21926
                     /gene="gppA"
                     /locus_tag="ACHKNP_RS09260"
                     /old_locus_tag="pgaptmp_001852"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HGU4704029.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21973..23994)
                     /gene="rep"
                     /locus_tag="ACHKNP_RS09265"
                     /old_locus_tag="pgaptmp_001853"
     CDS             complement(21973..23994)
                     /gene="rep"
                     /locus_tag="ACHKNP_RS09265"
                     /old_locus_tag="pgaptmp_001853"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HGU4704030.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24211..24660
                     /locus_tag="ACHKNP_RS09270"
                     /old_locus_tag="pgaptmp_001854"
     CDS             24211..24660
                     /locus_tag="ACHKNP_RS09270"
                     /old_locus_tag="pgaptmp_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGU4704031.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24859..25140
                     /gene="ppiC"
                     /locus_tag="ACHKNP_RS09275"
                     /old_locus_tag="pgaptmp_001855"
     CDS             24859..25140
                     /gene="ppiC"
                     /locus_tag="ACHKNP_RS09275"
                     /old_locus_tag="pgaptmp_001855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HGU4704032.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25227..26702)
                     /gene="ilvC"
                     /locus_tag="ACHKNP_RS09280"
                     /old_locus_tag="pgaptmp_001856"
     CDS             complement(25227..26702)
                     /gene="ilvC"
                     /locus_tag="ACHKNP_RS09280"
                     /old_locus_tag="pgaptmp_001856"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGU4704033.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26852..27745
                     /gene="ilvY"
                     /locus_tag="ACHKNP_RS09285"
                     /old_locus_tag="pgaptmp_001857"
     CDS             26852..27745
                     /gene="ilvY"
                     /locus_tag="ACHKNP_RS09285"
                     /old_locus_tag="pgaptmp_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HGU4704034.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(27797..29341)
                     /gene="ilvA"
                     /locus_tag="ACHKNP_RS09290"
                     /old_locus_tag="pgaptmp_001858"
     CDS             complement(27797..29341)
                     /gene="ilvA"
                     /locus_tag="ACHKNP_RS09290"
                     /old_locus_tag="pgaptmp_001858"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HGU4704035.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29344..31194)
                     /gene="ilvD"
                     /locus_tag="ACHKNP_RS09295"
                     /old_locus_tag="pgaptmp_001859"
     CDS             complement(29344..31194)
                     /gene="ilvD"
                     /locus_tag="ACHKNP_RS09295"
                     /old_locus_tag="pgaptmp_001859"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGU4704036.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31259..32188)
                     /gene="ilvE"
                     /locus_tag="ACHKNP_RS09300"
                     /old_locus_tag="pgaptmp_001860"
     CDS             complement(31259..32188)
                     /gene="ilvE"
                     /locus_tag="ACHKNP_RS09300"
                     /old_locus_tag="pgaptmp_001860"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HGU4704037.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32208..32471)
                     /gene="ilvM"
                     /locus_tag="ACHKNP_RS09305"
                     /old_locus_tag="pgaptmp_001861"
     CDS             complement(32208..32471)
                     /gene="ilvM"
                     /locus_tag="ACHKNP_RS09305"
                     /old_locus_tag="pgaptmp_001861"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HGU4704038.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32468..34114)
                     /gene="ilvG"
                     /locus_tag="ACHKNP_RS09310"
                     /old_locus_tag="pgaptmp_001862"
     CDS             complement(32468..34114)
                     /gene="ilvG"
                     /locus_tag="ACHKNP_RS09310"
                     /old_locus_tag="pgaptmp_001862"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HGU4704039.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34117..34167)
                     /gene="ilvX"
                     /locus_tag="ACHKNP_RS09315"
                     /old_locus_tag="pgaptmp_001863"
     CDS             complement(34117..34167)
                     /gene="ilvX"
                     /locus_tag="ACHKNP_RS09315"
                     /old_locus_tag="pgaptmp_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HGU4704040.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34254..34352)
                     /gene="ilvL"
                     /locus_tag="ACHKNP_RS09320"
                     /old_locus_tag="pgaptmp_001864"
     CDS             complement(34254..34352)
                     /gene="ilvL"
                     /locus_tag="ACHKNP_RS09320"
                     /old_locus_tag="pgaptmp_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HGU4704041.1"
                     /translation="MTALLRVISLVMISVVVIIIPPCGAALGRGKA"
     gene            34705..36225
                     /gene="yifB"
                     /locus_tag="ACHKNP_RS09325"
                     /old_locus_tag="pgaptmp_001865"
     CDS             34705..36225
                     /gene="yifB"
                     /locus_tag="ACHKNP_RS09325"
                     /old_locus_tag="pgaptmp_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HGU4704042.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36250..36588)
                     /gene="maoP"
                     /locus_tag="ACHKNP_RS09330"
                     /old_locus_tag="pgaptmp_001866"
     CDS             complement(36250..36588)
                     /gene="maoP"
                     /locus_tag="ACHKNP_RS09330"
                     /old_locus_tag="pgaptmp_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HGU4704043.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            36707..37546
                     /gene="hdfR"
                     /locus_tag="ACHKNP_RS09335"
                     /old_locus_tag="pgaptmp_001867"
     CDS             36707..37546
                     /gene="hdfR"
                     /locus_tag="ACHKNP_RS09335"
                     /old_locus_tag="pgaptmp_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HGU4704044.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(37642..37717)
                     /locus_tag="ACHKNP_RS09340"
                     /old_locus_tag="pgaptmp_001868"
     tRNA            complement(37642..37717)
                     /locus_tag="ACHKNP_RS09340"
                     /old_locus_tag="pgaptmp_001868"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37682..37684),aa:Trp,seq:cca)
     gene            complement(37726..37802)
                     /locus_tag="ACHKNP_RS09345"
                     /old_locus_tag="pgaptmp_001869"
     tRNA            complement(37726..37802)
                     /locus_tag="ACHKNP_RS09345"
                     /old_locus_tag="pgaptmp_001869"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37766..37768),aa:Asp,seq:gtc)
     gene            complement(37857..37972)
                     /gene="rrf"
                     /locus_tag="ACHKNP_RS09350"
                     /old_locus_tag="pgaptmp_001870"
     rRNA            complement(37857..37972)
                     /gene="rrf"
                     /locus_tag="ACHKNP_RS09350"
                     /old_locus_tag="pgaptmp_001870"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         9312 a        10371 c         9552 g         8819 t
ORIGIN      
        1 aaactaaaaa aagcatcaat aatcaacgcg atataataaa cctgccttac atatcaactg
       61 cgccagaggt aggattgaaa acgctctcct gattttccaa ttcattttct ggataaataa
      121 ataatttatt tttgtcacta ttatttatgt aatcatcctg tcagggagag ggatctcaat
      181 tatcaatgct taattacgtc atcattttga tgacatgaaa ccggagcatt ttgcatggaa
      241 ttttcgtttt cacccaaact tcttgttgtt gctgtcgccg ccgctcttcc tctcatggcc
      301 agcgcagcag ataccccgtc aactgccacc gcacgcaaag gctttgccgg atacgatcac
      361 ccaaaccagt atccacagaa cgctgcaatt tattgaattt gcacgatcat gtaggccgga
      421 taaggcgttt acgccgcatc cggcaatcaa ccgcaggcgg ccacaaaatg gccgcttttt
      481 tacttactca ccaccagcaa atgcgcatgc ataatgtcac tggccgggcg accgtgcgcc
      541 agcaaatcag ccattgcttt aagatatggc ggtagatggc ggaaataacg ctgatacccg
      601 gcacacaaat aattcagccc cggtttgccg ctggcatcga gcatgaagcg gtgtttcggg
      661 cagcctcccc agcacgcttt taacacgtta caactgcgac actgcgccgg taattgctta
      721 aatttatctt caccaaacac ctgctgttgc ggggaatcga tcatttctgc aattgtttgc
      781 tgatgcatat tccccagccg atattgcgga taaacatagt gatcgcaggc gtaaacgtcg
      841 ccgttgtgct caacaatcac cgagcgccca caggttggct gatgatggca aaccgcaccc
      901 ggcgcaccga caaaattggc aaacgcccat tcgatattca tcacgaaaat cttgccgacg
      961 tcgcgtttga tccagtggtc gaatatcgcc accagaaatt caccaaattc atcggggcgc
     1021 accgaccatt ccgtcagttc cccctgaata tcaccaggcg catgtagttt cagtccatcg
     1081 ctggcagctg tttcatcagc caggcgctcg accaccggaa taaactggat gaattcgact
     1141 cccgcatcgc acaaaaaatc ataaacctgc aacggttgct gcgcgctggt gcggttgacg
     1201 cagaccagca cgttatagtc gacatgatgt ttttgcagca gcgtcagggc acgcatcacc
     1261 agcttatgcg ttggtctgcc acctttggtc acgcgatatt gattgtggat ctcagccgga
     1321 ccgtccagcg ataacccaac aagaaaatga ttttctgcca gaaatgcaca ccatttatca
     1381 tcgagcagca cgccgttagt ctggaagctg ttacttatct tcctgccagc accgtatttc
     1441 gcctgtaact ttacggcacg gcggtaaaaa tccagcccga gtaacgttgg ttcgccgccc
     1501 tgccaggtaa aagccacttc gttttgcggt tcgctggcag cgatatagtg acggacatac
     1561 gcttccagcg tgtcatcgtc catatgcgtg actggctttt cgcggtaaag ggattgtttt
     1621 tcgagataaa aacagtagtc acagttcaga ttacaatcgg aaccactcgg tttcgccatc
     1681 acatgaaaag cacgcgttgg aacctgttgc agcatggtca ctccttgcct gaagtcatcc
     1741 ttcaatacgt taagggcgaa atcataccgt ggggcaatga gttattacgg gcgtttattg
     1801 cgaagggagg gtgacgaaat gcacagaaaa caaaaaaccc cgccgaagcg gggttcaaaa
     1861 ttggtcggcg agagaggatt cgaacctccg acccactggt cccaaaccag ttgcgctacc
     1921 aagctgcgct actcgccgaa atactgcttt ttgaattttt agttcaattc tttaaagtcg
     1981 tggtgcgagg ggggggactt gaacccccac gtccgtaagg acactaacac ctgaagctag
     2041 cgcgtctacc aattccgcca ccttcgcatt gtcacaactt ctaataatgg ggtggctaat
     2101 gggattcgaa cccacgacaa ctggaatcac aatccagggc tctaccaact gagctatagc
     2161 caccactaca aatcttgtta cgcggtatta ctaccaccgc agctcaagcg ccgggactaa
     2221 atggcgcgcc cgacaggatt cgaacctgag acctctgcct ccggagggca gcgctctatc
     2281 cagctgagct acgggcgctt agcgccgttg cgggggtgga taatacggac ttcccacccc
     2341 tctgtctagt ccctttttaa ataaattgcg cgtttggtta cggtttgtgc gttttgctga
     2401 ttattcctcc agtttatgcg ctttcccgtg gcgattaagg ccaaaaactt tataaatcgc
     2461 cgtcaccgcc agcataaaga tgatgccaac aaacagcgac atacgcgtat cttcattaaa
     2521 gtacatgccg atcaaaacgc aaatcaggaa tgccattgtt acgtagttgg cccacgggaa
     2581 caggattgag cggaacggat ggctggcaat cgccgcttta tgtgcacgcc gaaaacgcag
     2641 ctggcttatc aatatcacaa accatggcac catccccgga agcacgctgg cactgtagac
     2701 gtagacaaac acacgctgcg gattgggaat gatgtagttc aggcatgagc caattagcag
     2761 aatagcaata gatactgcca cacccgcaac tggtacgccg tgacgggaaa ctttcgccat
     2821 cgccgccggt aactgacggt ttttcgccag tgcgtagagc atacgtccgc aactgtacat
     2881 gccgctgtta cagccagaga gcgcagccgt cagcaccaca aagttgataa tgcccgccgc
     2941 tgcggtaata ccgattttgg caaaagtcag tacgaacggg ctgccgttgc tgccaatttc
     3001 attccacggg aagatggtga cgataacgaa aatcgcgcct acgtagaaaa tcaggatccg
     3061 ccacagcact ttgcctacgg cactgcgcag cgtcacctgc ggattcttcg cttcaccggc
     3121 agtaatgcca atcagctcca cgccctggta ggacgccacc acaatacaca gcgcggtcag
     3181 gaaccctttc cagccacccg caaagaaacc gccatgctct gtgagattgc taaaaccaat
     3241 cgactgcccg ccattgccaa agccaaagaa aatcacgccc aggccaatga caatcatcac
     3301 gataatcgtg gtgactttga tcatcgcgaa ccagaactcg atttcgccgt acaaccgcac
     3361 cgccgccaga ttcgccaacg ccaccagcgc cactgcgatc aatgcgggta tccactgcgc
     3421 catctccggg aaccagaact ggacataaac gccaatggcg gtgatttctg agatccccac
     3481 cgccatccac ataaaccagt aagaccaggc ggtgagataa ccaaagaacg ggctcatata
     3541 acgatgcgca taaacggcga acgaaccggt aaccggttcg aggaacaaca tttcgcccat
     3601 tgaacgcatg atgaaaaaga cgaatagccc ggcgatgata taggccaaca atacggatgg
     3661 cccggcccat ttcaaggtac tggcggcccc cataaacagg ccgacgccaa tggtgccccc
     3721 cagggcgatg agttcgatat gtcgagcttc cagcccacgc tgtagctctg gtttgttatc
     3781 tgccataaat cctcgtgttg tgtttgcatg ctttccggtg ttaccggtta tcgttatggg
     3841 tacatcgagt gttgcgaatg ttttcgtaat tcaggagaaa tggcaaataa agcattaaaa
     3901 atttgaatgc tttgtgtaat aaaaaagcag acaggcgacg gagtgaccac tccgtcgctt
     3961 tacaaagaga ggaaaatcag aggttgccgg tgtagtgcca gcgtaaataa cgcagcaaac
     4021 gaagctgacg cttaatgcgg ctcggctgcg aaagcaggcg gtagagccac tccagtccca
     4081 gcgtttgcca gattttcggt gcgcgtttta cgtgaccggt gaaaacatcg taagtcccgc
     4141 caacgcccat atacagcgca tctggatgca ccagacggca gtcgcgcatg aagatctcct
     4201 gctttggcga tcccatcgca acggtgacga tttgcgctcc gctggcatga atgcgttcaa
     4261 acagcgcctg acgctgctcg ggtttaaaat aaccatcctg actgccaacg atattcacat
     4321 tccactggtt gcgcagttta gcttcagttt gcgccagcac ttcaggttta ccgcccacaa
     4381 gaaataccgg cgtcccttct ttgcctgcgc gcgccatcag ctcttcccag agatcagcac
     4441 cggcaacgcg ggaaacctgc gcctgcgggt actttttacg tactgaacgt acaacgctga
     4501 tgccatccgc atatttaaat tcggcagcgt taattagctc cctgacctca gcattatctt
     4561 caatggtcag cattttttcg gcattaatgg caaccagcgt tccctgctta agctgcccgt
     4621 cagcaaacag ataatcgagg gcgtgctgca tatcacgcca accaatcaac tgtaagccac
     4681 gcagcgtata ggtcggtgcc atggtgttgt tattcattgt tatccttcaa cctgcgtccg
     4741 gagcgatgat tttgtacgtt tatgaatgag tccggcgctt tcaaaaagcc agtacaacag
     4801 ttttgcgatc atcagacatg cgccgaagac cacgataaaa aagactacgc gtgagacaaa
     4861 cgaatccagc ccttcacgtg ccagcacgat catattgaag atcgccccaa agcagaaact
     4921 gtgcaatatc gcagccttat agcgattagg ctcgcggttg cccagctcat acagccagtc
     4981 gaaccatttg atgatcagac caaccacgat cgccccgagc gggatgaaca acgcgccgcc
     5041 catcaccacc agtgagccta taagcgtagg cgagatcgcc agtccggagt ggttattcag
     5101 cacttcccag gtaaagtagt tggctgagtt cagcaccata ctcgggcgac ccggccacag
     5161 ccaggaaggg ataaagacat agaaatcgcg gacaattgga gccaggccct ggaagtcgat
     5221 gttgtcgtag ttctgcaaca gcaacgccag attctcccac ggcgagaagg tgtcgcgagt
     5281 gagatagaga aacgtataga acgcttcatc gccgctcaca ttcattccat agcgttttag
     5341 cgccagccag aacatgccca caatccccag cacgcccgcc gccgccagca tccacaacga
     5401 aatccagccg cgaataatgc caataaacag gaagatagcg aatgcgatga tgatattggc
     5461 gcgagtgccg ccgacaatca tataagtcag caagccaaag gcgaccgtgc tgacgaggaa
     5521 aaacagccac gctttgctat cctggcgcag aaagtagacc accagcatcg ccgggatgaa
     5581 aaagtaaaag aagcgtttta acgccacgcc ggagacttca ctggagaaga tctgactgta
     5641 ggagttaagc cggaacagca aaaagccgtt gtgcatgaag aagatgccga cgcttaccag
     5701 cgcgatgccc atcaggatca cccacgtaag attggtctcc acgcggttca tggtaaacag
     5761 cggacggcgc ggtacatcag caacgcgttt gcgtaggcgg gttttgtagg tgacatagta
     5821 aaccgcgtag aagcagcccg cagaaagcaa cgcctgcaac aagatttctg gcggcgcgac
     5881 accaacatca aagcgaaata ccagcacgct ggtcagcggg aagccgaaga aaaaggtgag
     5941 caaaaacagc aatgaaaaga agacattgaa gttaaagcgc acacggcgaa actcaaacca
     6001 ggtcagcgtg gcaataaaca gcgtgcagag cagccagacc acaaacaggc cactgaattg
     6061 cagcagactc atgcgccctc cccggcggca atcgccaacg cccgctgcca gccttgtaga
     6121 tagttagggc taaagaaggc aatggtgttt ttatccaccg acgccaactg gcgctgcgct
     6181 tcacgcacaa tatcctcgtt gagatcgtcg gtagtaaaca gcactggcaa atgctgttcc
     6241 gtcatatcct gccagaacgg attttcccgg ttaagcacac aaggaatgcc cgcctgaatc
     6301 agtaagcaca gcgtaccaat gccctgctgg cgagcaaaaa taaagtaacc aagatcgcac
     6361 tgacgaagta gcgccagata ggcgtcaaat tccagttttt cgctcaaaat ttgcagattt
     6421 tcttcgctga ataactccag ccccgcctga cgaacttcct caatgtacgc ttcgttatta
     6481 ggcggatatc ccatcggcac caccactttt accgtatcgc caaattgctg atgaacggca
     6541 cgtaaagcgg cgacatgctc attgctgagg tcgccggagt tccccaccag aatggtcatt
     6601 tttccttcac gttgccgatc gttcgccatc gtattgagag aagggtccat ccgcgtcggg
     6661 aagtacagca gttctccccg cacctttggg tgcgttttgg caaaaaagct caaatcgccg
     6721 cgggtggcaa atacacagcc gactcgcttt tgcgccaggc gacgtagtgg gtaaaaaagc
     6781 ttatatctca agccactgga aagctcatac aggtctgccc cccagatatg ccagaaaaac
     6841 tggctgggct taatgccacc actcagcaga gccagccaca gtgtgggatt gaactgaccg
     6901 tggaaaaaaa atcgctgctg acggttagct tttgctttcg cgatgaccgc ttccgccagc
     6961 gattttttcc cagggaaaaa ttgcacagaa agcgccggac agctatcact taagccgtcg
     7021 tccttgccaa caaccataaa ctcgcgcgcg tgctcgctcg tcgcagccag cgcgtcattg
     7081 aaaaaccgca aaacggttcg gttatggtga gggatatccg atcccagtac gtgaatcagt
     7141 acagtcatgc ccgcctacgc cagagtaaaa acacgccaca acaaagagaa aaatagacga
     7201 tataagttgc catatatgcc tgcgccgcgc ccagtgcacc atgcgcaggg attagccagt
     7261 gggcaaatac catcaataaa gtgaactggc tgatttccgc cagaatataa aaccgcaatg
     7321 acgctttggc gatcaccaga taaccaaaga cataagcgcc cacttttaac acatcaccca
     7381 ctaactgcca ggcaaagaga tcgcgcatag cggtaaattt attcgacaac agcagccaga
     7441 tagcaaaatc acgcagcagc cagacggtaa aactcgccgc cgccactgcc ggtaagacga
     7501 atttcagcga tttaaccact tcccgggtga tatcgcgctt ttccgttagc cgcgacaacg
     7561 tgggcagcaa atatacgctg aacgatgccg taataaattg caggtaggca tcggaaatac
     7621 tgctcacccc ttgccagatc cccacttcat cccagctata ctgcgccgcc agcagtttac
     7681 gcatcatgat gtaagcaaca ggcaaggtca ccgacgtaat caacgccatg agcgtaaatt
     7741 tgctcaactg ccctgccaga ccgttatccc agctgggttt cagatagctt aacgggatga
     7801 caccacgttt gatcaacatg atagtggcag gaattaccac cagcgcggga atcagcgcca
     7861 gacccagcaa cgccccttca taaccgccca aacggtaact gacgtagtac gcgagaacgc
     7921 caatcaagct gccgacaatc agcgataacg cattacctgc ggcatcgcga aagcctttca
     7981 tcagcgccag taacaggttg ccccaggcga tccccatttg caccagcgcc actaaacgca
     8041 ccagcccctg atagtcggta ttaccaaaca atccctggct gattggcgca gctgccagca
     8101 caaaaaccag cgccatcagc gtagagaaac caagtaccat cgctgatgaa gtgccgacca
     8161 cgcggcgcag ctgttgcgga ttatcatggt actgggcaac gtatttggtt acaccgttaa
     8221 agatgccagc tccggcaagc acgccgagca cggtaatcaa ctggcggaaa tttgccgcca
     8281 gcccaagccc cgccggacca aatgacacag ccagcaactt accgaccagt aacccggcac
     8341 caatcttgac cagtgtactg gccgccgtcc acaaggacgc ttttgccaac gacatatcag
     8401 gagaagtagt tcaacaaagt cgcaattacc gtacgctgat tgacgggcga caggttgtag
     8461 aacagcggca ggcgcagcag gcgctcgctc tctttggtgg tgtagcgatc ttcaccgtgg
     8521 aactcaccaa agcgttcccc cgcagggcaa ccgtgcagcg gaatgtaatg gaacaccgcc
     8581 atgatttccg cttctttcag aaagttaatc aacgcgctcc ggtcatcaat atcccgcagt
     8641 ttaatgtaga acatatgcgc gttctgcacg cagccatcgg gaatcgacgg cagctcgata
     8701 cgcccggctt tcgccagagg cgctaacgca tcgtagtagt tttgccacag cgccagacgt
     8761 tgctggttga tacgatccgc tgcttccagt tgcgcccaca ggtacgcagc ttgcagatcg
     8821 gacatcaaat agctggagcc aatatcgcgc caggtatatt tatcgacctg accacggaag
     8881 aactggctgc ggttagtgcc cttttcacgg atgatctcgg ctcgttcgat taacgcttta
     8941 tcgttaatca gcgtcgcgcc gccttcacca cccgccgtat agtttttggt ttcatggaag
     9001 ctaaagcagc caatatgacc aatggttccc agtgcacgcc ctttgtaagt ggacatcacg
     9061 ccctgagcgg catcttctac cacaaacaga ttatgctttt tcgccaacgc cataatggtg
     9121 tccatttcgc aggccacacc cgcgtaatgg accggcacga taacgcgcgt tttgtcggtg
     9181 atcgccgctt caatcagcgt ttcgtcgatg ttcatggtgt ccgggcgaac atccacaaaa
     9241 acgatttttg cgccacgcag cacaaaggca ttggcggtgg agacaaaggt gtagctcggc
     9301 atgatcactt catcgccagg ctggatatcg agcagcagcg ccgccatctc cagcgaagcg
     9361 gtgcaggacg gcgtcagtaa cactttggcg ctgccaaaac gttgctccag ccactgctgg
     9421 cagcgacggg taaaaccgcc atcgccacac agtttgccgc tacccattgc cgactgcata
     9481 tagtcgagtt cggttcccac caccggcggt gcgttaaatg gaatcatgtg atcacctgta
     9541 taaccagtac gcggtgcttt ctacattcgc accactttgt atgtatcgtt taagcgcggc
     9601 ggtgttgccc atttgggtcg ccacccgcaa agttgtttta ccgcgagcat acgcccagtt
     9661 tagcgccgtt tgcatcagct cagcacctac accgcgtcca gccagcaggc caattcgcgc
     9721 atctgtcgca ttgagttccc gtaaagagac ataaccgcga ataccgccgg acgccgcacg
     9781 taaaatcaga cattgatgat caaaggtgcc gcgcacggca ttttcaatcc actgtgcata
     9841 aaagcgtctg ctggcgtcag gcgcatacca cggcgcacga aaacggcttt gcgcaaatgc
     9901 ggcgctggct aactgacgta atgcgggaat atcggtctct tgtgccacta cagcaccact
     9961 atcactgaca ttgttcacgg gtagtgccaa atcaacttca ccttctacca gggagaatcc
    10021 cagctgttgc agggcatcca gttcacccgt atttgatgcc gcaattttgg cctgcacccg
    10081 tgaccacggc gctaacacgt ctggcgtcag gagcggtgct tcagacgtaa tgcgcacgat
    10141 ggcgctgtta acaccaaaga aggcgttttc ccaggttagt ggctcaatac tggcgcggac
    10201 gggcacgaag taactccagc agatattggc cgtagccagt tttcgctaat gaactggcag
    10261 cacgcttcac accctcgtca tcgagccagc cgttacgcca ggcgatctct tccaggcagg
    10321 caatcttaaa gccctggcgt ttttctactg tctgcacaaa ggtactggct tcaatcaggc
    10381 tgtcgtgagt gccagtgtcc agccaggcaa atccgcgccc gagcagttca acggtcaggt
    10441 tgcccgcctc gaggtacatc tggttgatgg aggtaatctc cagttcacca cgctccgacg
    10501 gcttcacctg ctttgcgtac tccacgactt tactgtcgta gaaataaagc ccggtcaccg
    10561 cccagtttga cttcggctgt tttggctttt cttccagcga gatagcgcgg aaattgtcgt
    10621 caaactccac cacgccaaag cgttccgggt ccatcacctg atagccaaat accgtcgccc
    10681 cttccgtgcg cgccgcaaca tgacgcagct tcggactgaa gccctgaccg aagaagatgt
    10741 tatcgcccag caccagacaa gaaggttcac cgttgaggaa ggtttcaccg atgataaagg
    10801 cctgcgccag accgtccggg ctgggctgtt cggcgtattc cagctggata ccgaactcac
    10861 taccatcgcc cagcaggcgc tggaaataac ctttatcttc cggcgtagtg atgatgagaa
    10921 tttcgcggat accggccaac atcagcaccg acagcggata gtaaatcatt ggcttatcgt
    10981 aaatcggcaa cagttgcttc gatacgccgc gcgtaatcgg atgcaatcgg gtgccggaac
    11041 cgcccgccag gataatacct ttcatgtttg cctccgctga aaattagcct ttcagaccta
    11101 aacgctcgcc ctgatagctg ccgtcctgca cctgcttcca ccagctttca ttagccagat
    11161 accactgcac cgttttacgc attccacttt caaaggtttc ctgtggcagc caaccaagtt
    11221 cacgggcaat tttcgaagca tcaatggcat aacgcagatc atgccccgga cggtcagcga
    11281 caaaggtgat caagtcacga tagtgcgcca cgccgtgcgg cttgttcgga gccagttctt
    11341 ccagcagctc gcaaatggtt tccacaacat cgagattctt acgctcgttg tgaccaccaa
    11401 tattataggt ttcaccgact ttcccggtgg tcgccacgca atacagcgcg cgggcgtgat
    11461 cttccacata cagccagtca cggatttgct gcccgttgcc atataccggc agcggtttac
    11521 ccgccagcgc gttgaggatc atcagcggga tcagtttttc cggaaagtgg taaggaccgt
    11581 agttattcga gcagttggtg atcagcgtcg gcagaccgta ggtccgcagc caggcgcgca
    11641 ccagatggtc gctactggct tttgacgcgg aataagggct gctcggcgca tacggcgtgg
    11701 tttcggtgaa gaaatcatcc gtcgagtgca ggtcaccata tacttcgtcg gtggagatat
    11761 gatgaaaacg gaacgctgat tttttatctt ccgtcagcgt attccagtaa gcccgcgccg
    11821 cttcaagcaa tgtataagtc ccgacaatgt tggtttcaat aaacgctgcc gggccgtcaa
    11881 tagaacggtc aacatggctt tcggctgcca gatgcatgac acagtctggc tgatgctcag
    11941 tgaatacgcg tgccagttct gcccgatcgc agatatcaac tttctcaaag gcaaagtgct
    12001 cgctttgcgc gaccggtgcc agcgacatca ggtttccggc gtaggtcagc ttatcgacca
    12061 ctaccaccgc atcgctcgtt tcgttgatga tataacgcac cagcgccgag ccaataaacc
    12121 cggcaccacc tgttatcaga atttttctca tcagcgccag actcctttgg catcgacgac
    12181 atactgctga tggacattgt cgccattgat aactttgaac tgactatgat cgaccagcat
    12241 caccagcacg tctgccgttg ccagcgcctc gtcaagctgc gccagagtac aaagcccggt
    12301 cagttttttc ggcaactggt ggatgttagg ctcaacaacc agcgtttcgc cgctgtgcca
    12361 ctgggcgatc agttcagcga tttccatcgc cgggctttcg cgcaggtcat caatattcgg
    12421 tttaaacgcc agaccaaagc aggcgatttt cagttcactg gcgcgtttat cggtagcagc
    12481 aaggcagtcg gcaaccgccg ctttcacctg atcgataacc cagaacggtt tgtgatcgtt
    12541 aacttcgcgc gcggtacgga taagccgcgc ctgctggggg ttctgtgcca cgataaacca
    12601 cggatcaaca gcaatgcagt gaccgcccac gccagggcca ggctgaagaa tattaacgcg
    12661 agggtgacga ttcgccaggc gaatcagttc ccagacgtta atcccctgat cggcacaaat
    12721 cagcgacaat tcattagcaa aagcgatatt cacatcgcgg aagctgtttt cggtgagctt
    12781 acacatttcc gccgtccgcg agttagtgac gacacactca ccttcgagga aaattttgta
    12841 cagttcgctg gcgcgggtcg aacaaaccgg cgtcatacca ccaatcacgc gatcgttttt
    12901 aatcagctcg accataacct ggcccggtaa cacgcgttcc gggcagtaag caatgttgac
    12961 gtccgcctgc tcgcccacct gctgtgggaa agtgagatcc ggacgcatct ctgctaacca
    13021 ttctgccatc ttctcggttg accccaccgg cgaggtggat tcaaggatca ccagcgcgcc
    13081 ttttttcagc actggcgcaa tggagcgagc agccgattca acgtaggtca tatctggctc
    13141 atgatcgcct ttaaacggcg tgggtacagc aatcagccag gcatccgctt caactggcgt
    13201 cgtgctcgct cgtaaaaaac cgccttctac ggcagttttt actacgctcg ccaaatcagg
    13261 ctcgacgata tggatttcgc cacgattgat ggtatcaacc gcgtgttggt tgatatcgac
    13321 accaatcacc tgtttttgcc gtgaggcaaa cgctgctgct gtaggcagcc cgatgtaacc
    13381 cagtccgata acagaaatgg tcgcaaaact catagtgata tccgattatt ttttaacgct
    13441 tccagaatgc gagagcatgc ctgaccatca ccatacgggt tatgggcgcg gctcatagtt
    13501 tgatattcgt tttcgtcttt taaaagacgc gtcacttcct cgacaattcg ctgcttatcc
    13561 gtgcctacca gacgcaccgt acccgccgtc accgcttccg gacgctcagt ggtatcgcgc
    13621 atcaccagca ccggtttccc cagcgaaggc gcttcttcct gaatgccgcc tgagtcggtc
    13681 aaaatcagcc aggcgtggtt catcagccag acaaacggta aatactcctg gggatcgatc
    13741 agaatgacat ttttcacatg ccccagaatg cgattgaccg gctctctgac gttcgggttg
    13801 agatgcaccg gatagacaat ctggatgtcc tggtgcgtgg tggcgatgtc tgccagcgcg
    13861 tggcagattt cttcaaagcc acgaccgaaa ctttcacgcc tgtgaccggt caccagaatc
    13921 atctttttat cggggtcgat aaacgggtaa tttgccgcca gttctgaacg cagcgtgtcg
    13981 ctgctcatca cctgatcacg cacccataac agtgcatcaa tgactgtatt accagtaatg
    14041 aagattcggc tatccgcaac gttttcacgc agcaagtttt gccgggaagt ttcggttgga
    14101 gagaagtgat acatcgccag atgcccggtc aatgtacggt tagcctcttc cggccacggc
    14161 gaatagagat cgcccgtgcg taaaccagcc tcaacgtgac caacaggaat acgctgataa
    14221 aacgccgcca ggctggttgc cagcgtcgtt gttgtgtcgc cgtgaaccag cacgacgtct
    14281 ggtttgaact cggcaagaat aggttttagc ccttccagaa tccgacaggt tatctctgtc
    14341 aggccctgtc ctggctgcat tatgttgaga tcgtagtcag gtacaatgga aaagagtttc
    14401 agcacctgat cgagcatctc ccgatgctgc gcagtgacgc aaactttagc ctcaaaaaaa
    14461 ggatcttttg ccaacgcatg caccaacgga gccatcttga tggcttccgg gcgcgtacca
    14521 aatacagtca gtactttcac atcgattctc ttcgaataag cggcgagcgc ctttgcgctc
    14581 gccgcagcag tgttgctatt tcgagcaacg gcgggttaat gcgacaccag ccccgatcag
    14641 ccccccgaca atgccccaca taatcatcag gaaggcacga cgtgggctat cgcgttttac
    14701 cggttcttcc ggcgtacgca aatagcgata ggtctgaaaa cgcggatcca gagttggacc
    14761 aacattcagg gtgtttaaca tggcccgatt ctgatcatag tcgagatcaa aggccggacc
    14821 gacggcctgt aaattttcca gtcgagcctg gagcattgga cgcccaagca ggaacatttc
    14881 tgaatcaggt aattcctcgg caggcacatc tgtcgcactg cgcgaaatat tatgctgctc
    14941 agcaattttc agcgcctgct caatgctgtt catccggcgg tcgtagatgg ctttcgccac
    15001 ctcttcctga cgcttcacct gagctttcat ctggatggtg cgtgccgccc atgcgccttt
    15061 cagctcctca ttcagatggc tggctgcacg ctggctggca aaagcaacat actgacgtaa
    15121 caggttatta gcgtcaggcg cggtttcggc aataagcttc acgctgtcat tgaccgcgcg
    15181 ggtaaagtct ccggggataa actggatgtt gttaatcatt tcatccagca acgccgcatc
    15241 ggctttgctg ttgcccacca tccgctgctt gtaatagtcg gtttgcagcc agaactctct
    15301 gcgggtatcc cacgaggcca gttgcataac aaactcttta taggcttcgt ccatgaccga
    15361 tggttggtcg gcagaagcca tgtttgaacg gacatccagg ttacgcaaaa attgctgctg
    15421 cgagtaatat ccccccagca tattcaccgt tggacgatcg gtaatcgccg tcgcgctcca
    15481 ctcttgtcga gcaaaaaaag tatacgccag cgcgattaac gcaaacgcca gccccatgcc
    15541 aataatccat agcttcccag cccacaaggt acgaaacaac ccacgaatat ccagttcatt
    15601 ttcagcgtct tcggccggtt tcccaggcat tggttgtgtc atcacatcct catttatttg
    15661 gttaaattgg ggctgccacc acgatttcta cgcagtctgc gttttacgcg cttaataaag
    15721 cgagcaactt tccaggcacg cttaatgcaa tatccataga ggaagaatgc tagcaaaaag
    15781 agcaccagca tgacccactc cgggacaaaa tgagaatatt ctgccagcac gccaatggaa
    15841 gcgagcagtg ctgcggcaag ggtaatcagc acaaacgcct gacgggaagt aaacccggca
    15901 cgcatgatca aatggtgaat atgctgacgg tcaggagaga atgggctcat gcctttacgc
    15961 aggcgacggt acataatcgc caccatatcc attagcggaa tggcgattat ccacaaagcg
    16021 gtaaccgggc tgatgggatg ggttttgccc tgggtcgttt cgagcaggat ccagataacg
    16081 gtaaaaccaa tcagcgtact gcccgcatca cccataaaga ctttgtagcg gcgacccagg
    16141 ataccaaggt taagcatgat gtatggcagg atggcggcga tcatcgcaaa gcaccagatt
    16201 gcgaggctgg tttgcccgtc gaaccacaaa atcataccga ttgctgcaaa cgagacgcag
    16261 gacaacccgc ccagcaagcc atcaatgcca tcaaccatgt tgaacgcatt aatggccgcc
    16321 cagacggcaa atagcgtcag gaagtaacca aacggtccga gcaccatctc ccaggagcca
    16381 aagatataac ccaggctact gagataaagt ttgccgaaca ccatcataac aatgccaaca
    16441 gcggcctgta tggtggcacg gatttttacg ctgatatcaa aacggtcatc cagcgcgcca
    16501 atgaaaacaa gcacaccggc acaagcgaga tagagagatg catgcggaat atagtaatcg
    16561 acaattccga acgtgaagca aatccctgcg taaaccgaaa tccccccaac gagaggtatc
    16621 aatccctggt gacgtttgcg gaagtttggt ttatccacta aaccgacttt ttttgccact
    16681 ttacgggcaa aaaacagaaa cagtgtcgtg aataaaaaaa tactgatgag atcagtactc
    16741 actgtcagta aattcacaat gcatgctctc agagaaaatt attagcagaa gtataaccac
    16801 gaagaccttt attcagaagg gaaagtccgt tccgaaccca ccagtcctgt gcaaaaatta
    16861 accatttgat atgcaaattg cataacatgc cagcaaattc caggaatttt cctaacatcg
    16921 ccagcgcggc attaagatta cagatataaa agcaaaacgc cacgtaaaca cgtggcgttt
    16981 ttggcataag acaaatttat gagcgtttca tcatttcgaa gaaatcgtca ttggtcttag
    17041 tcatggccag tttattaatg aggaattcca ttgcatcgat ttcgcccatc gggtgaatga
    17101 ttttgcgcag gatccacatt ttctgcagtt cttcctgagt cgtgagcagc tcttctttac
    17161 gggtaccgga acggttgtag tcgatagccg ggaagacgcg tttttcagcg atcttacgag
    17221 agaggtgcag ttccatgttg cctgtacctt taaactcttc gtaaataact tcgtccattt
    17281 tagaaccggt atcgataagc gccgtcgcga taatggtcag gctgccgccc tcttccacgt
    17341 tacgcgccgc accgaagaag cgtttcggac gatgaagggc attggcgtcc acaccaccgg
    17401 tcaacacttt acctgacgcc ggaacaacag tgttgtaagc gcgcgccaga cgagtgatgg
    17461 agtcgagcag aatgataacg tctttcttgt gctcaaccag gcgtttggcc ttctcgatca
    17521 ccatttccgc aacctgaacg tggcgagatg cgggttcgtc aaaggtagaa gcaacaactt
    17581 caccttttac cagacgctgc atctcggtta cttcttccgg acgttcgtcg atcagcagaa
    17641 ccatcagcac acaatccggg tggttgtaag caatgctctg agcaatgttc tgcagcagca
    17701 tagttttacc ggctttcggc ggtgccacaa tcagaccacg ctgaccacga ccgataggtg
    17761 atgccagatc cagtacgcga gcggttaaat cttcagtaga accgttacca cgttccatac
    17821 gcagacgaga gtttgcgtgc agcggggtta agttctcaaa gaggattttg ttgcgggcgt
    17881 tttcaggttt gtcgaagtta acttcgttaa ctttcagcag cgcaaaatag cgttcacctt
    17941 ctttcggcgg gcgaatctta ccagagatgg tatcaccagt gcggaggttg aaacggcgga
    18001 tttggctagg ggaaacgtag atgtcatcag gaccggcgag gtaggagctg tctgcggaac
    18061 ggaggaaacc aaatccatcc tgcaatatct ccagtacgcc atcaccaaag atatcttcgc
    18121 cactctttgc gtgctgcttc aggatggcaa aaataatgtc ctgcttacgc atacgagcca
    18181 ggttttccag ccccatattt tcgccgagag tgatcagctc agaaaccggc gtattcttta
    18241 attcggtaag attcatagtg gtgtgagttc ttaaacttgg ggtaaatctc gaacttaatg
    18301 ttgtgaatgg tatggcagga tcatccatgc ctgtttacgg tcatcgactc atgtcttttc
    18361 gctgcctggt cacaggagag tacgcagaac tgaaacgaca agacggattg agtgacgagc
    18421 cagaaatctg ttcacttcgc atttaagata aaacgggaag cgttggaaat tacaagattc
    18481 aaacttaata aggtatgttt aatacgaagt caacactaag ttagcatgac tcacgccggg
    18541 cgtccagttt ttagcgacgg ggcacccgaa catgaaattc ccttacgcca ggttagcgtc
    18601 gaggaactct ttcaactgac ctttagacag tgcacccact ttggttgccg ccacttcacc
    18661 gtttttgaac agcagcagag tcgggatacc acggatgcca tatttcggcg cagtgccagg
    18721 gttttgatcg atgttcagtt ttgcaacggt cagtttgccc tgatattcgt cagcgatttc
    18781 atccagaatc ggggcgatca ttttgcacgg accgcaccac tctgcccaga aatcgacgag
    18841 gatcgccccg tccgctttga gtacatccgt gtcaaaactg tcgtcagtca ggtgaataat
    18901 tttatcgctc atatataact ccacaggaat aagcctggcg tgttggtttc gttgttggtg
    18961 taacattaac caactaaagg ttgactttat ttcaccggat acgctttcgt aaagcaatag
    19021 taagctgata ttctaccaca ctatgagcaa aacacattta acagaacaga agttttccga
    19081 cttcgccctg catccgaagg ttgtagaagc ccttgaaaaa aaagggtttc ataactgtac
    19141 gcccattcag gcactggccc ttccgctgac gctggcgggt cgtgacgtag ccgggcaggc
    19201 gcaaaccggt accgggaaaa cgatggcgtt tcttacgtca acgtttcatt atcttctctc
    19261 tcatcctgcg attgccgatc gcaaggtgaa tcaaccacgt gccttaatta tggcaccgac
    19321 gcgtgaactt gccgtgcaga tccatgccga cgcagaaccg ctggcggaag ctaccggcct
    19381 gaagctgggt ctggcttacg gtggtgatgg ctacgacaaa cagctgaaag tgctggaaag
    19441 cggcgttgac attctgattg gcactacggg gcgtttaatt gactacgcca agcagaacca
    19501 cattaacctc ggtgccattc aggtggtggt actggacgaa gccgatcgca tgtacgatct
    19561 gggctttatt aaagatatcc gctggctgtt ccgccgtatg ccgcctgcaa accagcgcct
    19621 caacatgctg ttctccgcca cgctttcgta ccgggtacgt gaactggcgt tcgagcagat
    19681 gaacaatgcc gaatatattg aagtggaacc ggaacagaaa acgggccacc gtattaaaga
    19741 agagcttttc tacccttcta acgaagaaaa aatgcgtttg ctgcaaacgc tgatcgaaga
    19801 agagtggcca gacagagcga ttattttcgc caataccaaa taccgttgtg aagagatctg
    19861 gggccacctg gcggcagatg gtcatcgtgt cggtttgttg acaggcgatg tcgcgcagaa
    19921 aaaacgtctg cgtattcttg atgaatttac ccgtggcgat ctggatattc tggttgccac
    19981 cgacgttgcc gcgcgtggtt tgcatattcc ggcagtgacg cacgtcttta actacgattt
    20041 acccgatgac tgtgaagatt acgttcaccg tattggtcgt acaggtcgcg caggcgcaag
    20101 cggtcactct atcagcctgg cgtgtgaaga gtatgcattg aatttgcctg ctattgagac
    20161 ctatattggt cactcaattc cggtaagcaa atacaatccg gacgcattga tgaccgatct
    20221 gccaaaaccg ctgcgcctca cgcgcccacg tacaggcaat ggtccgcgtc gtactggcgc
    20281 tccgcgtaat cgtcgtcgtt caggttaaat aaaatgtcat actcaaaatc attcaaactg
    20341 tatcaagatg gcaagagaat gaatcccgat gagcatactg tagtaagtga ttcaggtgaa
    20401 agaatgcagc caacacagag acagattgaa ggatgaagag tatgggttcc acctcgtcgc
    20461 tgtatgcagc cattgatctc ggttcgaata gttttcatat gctggttgtg cgcgaggtgg
    20521 ctggaagcat ccagacgctg acgcgaataa aacgcaaagt gcgtctggct gctggcctga
    20581 atagcgaaaa tgccctgtct aatgaagcaa tggagcgcgg ttggcaatgt ctgcgcctgt
    20641 ttgctgaacg tctgcaagat atccctccct cgcaaattcg cgttgtcgct acggcgacat
    20701 tacgtcttgc cgtcaatgcg ggtgatttta ttgccaaagc gcaggaaatc ctcggttgtc
    20761 cggtacaggt gatcagcggt gaagaggaag cacgtctcat ttatcagggc gttgctcaca
    20821 caactggcgg tgccgatcag cgcctggtgg tggatatagg cggtgccagt actgaactgg
    20881 taaccggcac gggtgcacaa accacctcgt tgttcagcct gtcgatgggc tgcgtcacct
    20941 ggctggaacg ctattttgcc gatcgtaatc tggggcagga aaattttgat gctgcagaaa
    21001 aagcggcacg cgaagtgtta cgtccggttg ccgatgaatt acggtatcac ggctggaaag
    21061 tgtgcgttgg tgcttccggc accgtgcagg cgttacagga aatcatgatg gcacagggga
    21121 tggatgaacg cattaccctg gaaaagttgc agcaattgaa acagcgagcc attcattgcg
    21181 gtcggctgga ggaactggag attgacgggc tgacgctgga acgtgcgtta gtgttcccga
    21241 gtggtctggc gatcctgatc gccattttta ccgaactgaa tattcagtgt atgaccctgg
    21301 cgggcggtgc gctgcgtgaa ggcctggtct acggcatgtt gcatctggcc gtcgagcagg
    21361 atattcgcag ccgtacgctg cgtaatattc agcgccgctt tatgatcgat attgatcagg
    21421 cgcagcgcgt agccaaagtt gcggctaact tcttcgatca ggtggaaaag gaatggcatc
    21481 ttgaagcaat aagccgcgat ttgctcatca gcgcctgtca gcttcatgaa atcggcctga
    21541 gcgttgactt taaacaagcg ccgcaacacg ctgcttatct ggtgcgtaat ctggatcttc
    21601 ccggttttac ccccgcacag aaaaaattgc tggcgacgct actgctcaac cagactaatc
    21661 cggtcgatct ctcatcgctg catcagcaaa atgccgtacc tccgcgcgtc gcagaacaac
    21721 tctgccgttt actccgcctg gccatcattt ttgccagccg tcgccgtgac gatctcgtgc
    21781 cagagatgac attacaggct aaccatgaac tgttgacctt gacgcttccg caaggttggc
    21841 taacccaaca tccgctgggt aaagagatta ttgctcagga aagccagtgg cagagctatg
    21901 tccactggcc gctggaagtg cattaatgag taagtgccgg atgcgatgct gacgcatctt
    21961 ttccggcctt gattatttcc ctcgttttgc cgccatcatc gctttcagat tcgccagatg
    22021 gctttgccct ttctgcatcc gttcttcggc gctgaccact ttgcgctcct gttcccaaat
    22081 cagatcatcc tgcggcagct ccagcaaaaa gcggctcggc tccgggcgca ccagctcgcc
    22141 gtactgacgg cgttctttac acagcgtaaa ggtcaattcc ttctgggcgc gggtaatgcc
    22201 gacataggcc agccgccgct cctcatcgat attatcttca tcgatgctgc tctggtgcgg
    22261 caaaaaccct tcttccatac cgaccatgta gacataagga aactccagcc cttttgacgc
    22321 gtgcagagtc atcagttgca cctgatccag ctcttcttca ctctcaccac gctccatcat
    22381 gtcgcgcaaa gtaaagcgcg tcaccacctg ggtgagcgtc atcggctcat ccagttcgct
    22441 gccttccagc atctccgtca tccagctaaa cagttggttg acgttcttca tgcgcatttc
    22501 ggcggctttc gggctgggcg atgtttcgta cagccaggat tcataatcca tgccgtggat
    22561 cagatcgcgc accgcggcaa tcggctcccg ctccgccaga cgctggattt ctgccaacca
    22621 gtgagtaaag cgggtcaatg cttcataacc acgtccgcta agcgtctgac tcaggcccat
    22681 atcaaagctg gcggtaaaca tgcttttatt gcgcgtcatc gcccactcac ccagcttttt
    22741 cagcgtcgcc gggccaatct ctctcttcgg cgtgttaacg atacgcagaa atgcgctgtc
    22801 atcgtccggg ttagtcagca cgcgcagata agccagcaag tccttgattt caggacgaga
    22861 gaaaaacgac gtaccaccag aaattttgta cgggatgcgg ttttgcatca ggaatttttc
    22921 aaacactcgc gactgatggt taccgcgata aagaatagcg taatctttgt actgcgtttt
    22981 attgacgaag tgatgcgcaa tcagttcacc agtgacgcgc tcggcttcat gttcttcgtt
    23041 attcgcgctt aaaactttaa gctctgtgcc gtaacccagt tcggagaaca gacgcttctc
    23101 aaagacgtgc gggttattgg cgatcaggat gttcgccgct ttcaaaatac gtccggaaga
    23161 gcggtagttc tgctcaagct taatcacctt cagcgccgga aaatcctgac tcagcagcac
    23221 caggttttgt ggacgtgcgc cgcgccagga gtagatcgac tggtcgtcat cgcccaccac
    23281 ggtaaagcgc gcgcggctgc ccaccagcag tttcaccagt tcatactggc tggtgttggt
    23341 gtcctgatac tcatccacca gcagatagcg aattttgttc tgccagcgct cgcggacttc
    23401 ttcattgcgt tgcagcagca acgtcggcaa taaaatcaga tcatcgaagt cgagaacatt
    23461 gcacgctttc aggtgtgcat catacagccc ataacaatgg gcaaaaatac gatcccgctc
    23521 gccctttgcc tctgccgccg cctgcgccgg agttttgaga tcgttcttcc agttggagat
    23581 ggtcgaaatc agctgttgca ggagaacttt gtcatcttca atcagcccct cggtcaactc
    23641 tttaagcaaa gcaagctgat cggtatcgtc aaacagcgaa aagttcgcct tcatcccaag
    23701 cgccgcatac tcgcgtttga tgatatccaa ccccagcgtg tgaaaagtgg agatcatcaa
    23761 cccgcgcgcc tctttgcgac ccagcgtctg cccgacacgc tctttcatct cgcgcgccgc
    23821 cttattggta aaggtcaccg ccgcgatatg tcgcgcctga taaccgcaac cgcggatcag
    23881 atgggcgatt ttattggtga tcacacgagt tttaccggaa cccgcgcccg ccagcaccag
    23941 gcaggggccg gtaacgaatt cgacagcttg ttgttggccg gggtttagac gcataggtgt
    24001 attgctcaat cttcgaacgg ggggaggatt gtagcagaat cgcatttgaa tgtgaccgcg
    24061 tgtaagccgc taaccggttg gctgcggatg tttaaatcag agagagaaga ccgaaatcgg
    24121 agttcatcgt gaatccatta gacttagaaa atatcgggtc gtaacgttgt gttattactt
    24181 tttcagagtt tactaaggag aggtttactc gtggcgatta atttcagccc taaggttggt
    24241 gaaatacagg aatgcaactt tggtaactat cctgttatcc agaatggaca gttcagcacc
    24301 acgtattacg atggccgcat tcctcctgaa atgataaaaa acaggttagt tgtcgtgctc
    24361 aacggaaaaa ttaatggcaa cgcatgtatc gtcgttccat tatcaaccac tcgcgatcat
    24421 gacaagttaa atcgtggaat gcatgttgaa atagccagta atgttatcaa tggcctgcaa
    24481 ttctttgatc agcagattcg ttgggccaaa gccgacctgg tacaacaagt cagtcgaaac
    24541 cgattaaata gagcaaggac ttacagaggg tatcttaatc aatgtttgcc acatgagttg
    24601 gttgcagaca tacagagggc ggtcatcaag tcaatcaatg ccatatcgct cataaattga
    24661 caataaaata accatccccg ataatgactt tgtagcccat agaggcccta agttttctcc
    24721 ccttgaggga cctgcgaagc ccggattttc cgggcttttg ccatatatgt accagcccct
    24781 ccctgactga caattacgtt agcatctgcg gatcatgcta aaatcgccgc ctgacaatta
    24841 ttcactcaag gcacgatcat ggcaaaaaca gcagcagcac tgcatatcct tgtaaaagaa
    24901 gagaaactgg ctctggatct tctcgagcag attaagaacg gtgccgattt cggcaagctg
    24961 gcgaagaaac actctatttg cccgtcaggc aaacgcggcg gtgatttagg tgaattccgc
    25021 cagggtcaga tggttccggc gttcgataaa gtggttttct cttgtccggt actggagccg
    25081 accggcccgc tgcacaccca gttcggatat cacatcatta aggtgctgta ccgcaactaa
    25141 tagcaaggcc ttctccagga gaaggccttg agtgttttct ccctctccct gtgggagagg
    25201 gtcggggtga gggcatcagc gcgcacttaa cccgcaacag caatacgttt catatctgtc
    25261 atatagccgc gcagtttctt acctacctgc tcaatcgcat ggctgcgaat cgcttcgttc
    25321 acatcacgca gttgcgcgtt atctaccgcg ccttccggaa tagctttacc caagtcgccc
    25381 ggttgcagct ctgccataaa cggtttcagc aacggcacac aagcgtaaga gaacagatag
    25441 ttaccgtact cagcggtatc agagataacc acgttcattt cgtacagacg cttacgggcg
    25501 atggtgttgg caatcagcgg cagctcgtgc agtgattcat aatatgcaga ctcttcaatg
    25561 atgccggaat cgaccatggt ttcgaacgcc agttcaacgc ccgcttttac catcgcgatc
    25621 atcagtacgc ctttatcgaa gtactcctgc tcgccgattt tgccttcata ctgcggcgcg
    25681 gtttcaaacg cggttttgcc ggtctcttca cgccaggtca gcagtttctt atcatcgttg
    25741 gcccagtccg ccatcatacc ggaagagaat tcgccggaga tgatgtcgtc catatgtttc
    25801 tggaacagcg gtgccatgat ctctttcagc tgttctgaca gcgcataagc acgcagtttc
    25861 gccgggttag agagacggtc catcatcagg gtgatgccgc cctgcttcag cgcttcggtg
    25921 atggtttccc aaccgaactg aattagtttt tctgcgtatg ccggatcggt gccctcttcc
    25981 accagcttgt cgaagcacag cagcgagcca gcctgcaaca taccgcacag gatggtttgc
    26041 tcacccatca ggtcagattt cacttccgca acgaaggacg attccagcac gcccgcacgg
    26101 tgaccaccgg ttgctgccgc ccatgcttta gcgatcgcca taccttcgcc tttcggatcg
    26161 ttttccgggt gaacggcaat cagcgtcggt acgccgaatc cacgtttgta ctcttcacgc
    26221 acttcggtgc cagggcattt cggcgcaacc atgactacgg tgatatcttt acggatctgc
    26281 tcgcccactt cgacgatgtt gaaaccgtgc gagtaaccca gcgccgcgcc gtctttcatc
    26341 agtggctgta cggtgcgcac tacatcagag tgctgcttgt ccggcgtcag gttaaccacc
    26401 agatccgcct gcgggatcag ttcttcgtaa gtaccaactt taaaaccgtt ttcggtcgct
    26461 ttacgccagg acgcgcgctt ctcggcaatc gcttctttac gcagagcgta ggagatatcc
    26521 agaccagaat cacgcatgtt caggccctgg ttcagaccct gtgcgccaca gccgacgatg
    26581 actacttttt taccctgaag gtagctcgcg ccatcggcga attcatcgcg ccccataaag
    26641 cgacatttgc ccagctgtgc cagctgctga cgcagattca gtgtattgaa gtagttagcc
    26701 atggtgattc ctcgtgatgt tgtgcttctt attgttcggt tcgcgtttgc gagattgaat
    26761 tcactatatg acaggaaatt tattgcggaa attgatatat tcacaacgtc acattgcaat
    26821 ttttgcaacg tcaacatcga gggctgcgcc attggattta cgcgatctga aaaccttcct
    26881 gcatctggcg gaaagccgcc attttggccg cagcgcgcgg gcgatgcacg ttagcccatc
    26941 cacgctctca cggcagattc agcgcctgga agaagatctt ggtcagccgc tatttgtgcg
    27001 cgataaccgc acggtaacgc tgacggaagc gggcgaagag ctgcgcgttt tcgcccagca
    27061 aacgctgttg cagtatcagc aattgcgcca caccatcgat cagcaagggc cgtcgctctc
    27121 tggcgaatta catatcttct gctcggtgac cgctgcctac agccatctgc cgccgattct
    27181 ggatcgcttc cgcgcggaac acccgtcggt ggagatcaaa ctcaccactg gtgatgcggc
    27241 agatgcaatg gaaaaagtgg tcacgggaga agcggatctg gcgattgccg ggaaaccgga
    27301 gacgctaccc ggcgctgtgg cgttttcgat gctggagaat ctggcagtag tgctgattgc
    27361 gcccgcgctg ccctgcccgg tgcgtaacca ggtttcggca gagaagccgg actggtctac
    27421 agtgccgttt atcatggccg atcagggacc ggtgcgtcgc cgcattgaac tgtggtttcg
    27481 acgcaataaa atcagcaacc cgatgattta cgccacggtt ggcgggcatg aagcgatggt
    27541 atcgatggtg gcgctcggct gtggtgtggc gctgttgccg gaagtagtgc tggaaaacag
    27601 ccccgaaccg gtgcgtaacc gcgtgatgat tttagagcgc agcgatgaaa aaacgccgtt
    27661 tgaactgggc gtgtgtgcac aaaaaaagcg gctgcatgag ccgctgattg aggcgttctg
    27721 gaggattttg ccgaaccaca aatgacgttg tcgcgcgggt aggcctgata agcgaagcgc
    27781 tatcaggcat ttttccctaa cccgccaaaa agaacctgaa cgccgggtta ttggtttcgt
    27841 cgtggcaatc gtagcccagc tcattcagcc gggtttcgaa atccggttca tggtcgccaa
    27901 gttcgaacgc cgccagtacg cgcccgtagt cggtgccatg gctgcgatag tggaacaaag
    27961 aaatgttcca gtgcgtaccc agcgtgttga ggaagcgcag cagcgcgccc ggtgattccg
    28021 ggaattcgaa gctgtagagg cgttcctgca acggatgtga cggacgcccg ccgaccatat
    28081 agcgcacatg caacttcgcc atttcgtcgt cggagagatc caccacgctg tagccgccgt
    28141 cgttgagcat ctgcaaaatt tctttgcgct cttcgaggcc gcggctcagg cgcacgccga
    28201 caaagatgca ggcgtttttg gcatcggcaa aacggtagtt gaactcggtg accgatcgcc
    28261 cgccaagcag ttggcagaat ttgaggaagc tgcctttttc ttccggaatg gtcaccgcca
    28321 acaacgcttc acgctgttcg cccagttcgc agcgttccga gacgtagcgc aggccgtgga
    28381 agttcacgtt ggcaccggaa agaatatgcg ccagccgttc gccgcgaatg ttgtgctggg
    28441 cgatatattt tttcattccc gccagcgcca gcgcgccaga gggttccgcc accgcgcgca
    28501 catcttcgaa taaatccttc atcgccgcac agatcgcatc actatcgacg gtgatgatgt
    28561 cgtcgagata ctcctggcat aaacggaagg tttcgtcacc gatgcgtttt accgctacgc
    28621 cttcagcaaa tagccctacg cgcggcagat caaccggatg acccgcatcc agcgctgctt
    28681 tcaggcaggc ggagtcttcc gcttctacgg cgatcacttt gatttgtggc atcagttgtt
    28741 tgatcagcac cgcaacgccc gccgccagac cgccgccgcc gactggcaca aatacgcggt
    28801 cgagatgggc gtcctgctgg agcagttcca gcgccagcgt gccttgcccg gcaatcacca
    28861 tcggatggtc gaacggcggc acccaggtga acccctgctg ctgtgacagt tcgatcgctt
    28921 tagctttcgc ttcgtcaaag ttcgcgccat gaagcagcac ttcgccgccg aatccacgta
    28981 ccgcatcgac tttgatgtcg gcggtggcgg ttggcataac gatcagggcc ttcacgccta
    29041 accgcgcaga agaaaacgcg acgccctgcg cgtggttacc cgcagaagca gtgatcacgc
    29101 cgtgcgcttt ttgttcttcc gtcaggcccg ccatcatggc gtatgcgccg cgcagcttaa
    29161 agctgtgcac tggctggcga tcttcgcgct tcaccagaat gacgttatca agacgcgacg
    29221 acagtttttc cattttttgt agcggcgtaa cctgcgccgc ctcgtaaacc ggcgcgcgca
    29281 gcactgctct taaatattcg gcaccttccg gagcaccgga caggggttgc gagtcagcca
    29341 ttattaaccc cccagtttcg atttatcgcg caccgcacct ttgtcggcgc tggttgccag
    29401 gctggcgtag gcgcgcaggg caaaggaaac ctgacgttca cggtttttcg gcgtccaggc
    29461 tttgtcacct cgagcgtcct gcgcttcacg acgcgccgcc agttcggcat cgcttacctg
    29521 taactgaatg ccacggttcg ggatgtctat agcgatcagg tcaccatctt caatcaggcc
    29581 aatgctgccg ccgcttgccg cttccggtga gacgtggccg atggaaagac cagaggtgcc
    29641 gccagagaaa cgaccgtcgg tgatcagcgc acaggcttta ccgagaccca ttgatttcag
    29701 gaagctggtt gggtagagca tttcctgcat gccagggccg cctttcgggc cttcatagcg
    29761 gatcaccacc acatcgcccg cgacaacttt gccaccgagg atcgcttcta ccgcgtcgtc
    29821 ctggctttcg tacactttcg ccgggccggt gaatttgagg atgctgtcat cgacgcccgc
    29881 ggttttaacg atgcagccgt tttctgcgaa attaccgtag agcaccgcca ggccgccgtc
    29941 tttgctgtag gcgtgttcca gcgagcggat acagccattg gcgcgatcgt catcgagaga
    30001 atcccaacgg caatcctgcg agaatgcctg tgtggtacga atgcctgcag gacctgcgcg
    30061 gaacatattt tttaccgcgt catcctgggt cagcataacg tcgtattgtt ccagcgtttg
    30121 cggcaacgtc aggccaagta cgtttttcac atcacggttc agtaaccccg cgcgatccag
    30181 ttcgccgaga ataccgataa caccaccagc acggtgaaca tcttccatat ggtatttctg
    30241 ggtgctcggc gcaactttac acagctgtgg aaccttgcgg gaaagcttat cgatatcact
    30301 catggtgaag tcgatttccg cttcctgcgc cgccgccagc aggtgaagta cggtgttagt
    30361 cgatccaccc atcgcgatat ccagcgtcat ggcgttttca aacgccgctt tgctagcaat
    30421 attgcgcggc agtgcacttt cgtcgttttg ctcgtagtaa cgtttggtca attcaacaat
    30481 gcgtttaccg gcattgagga acaactgctt acggtcggcg tgggttgcca gcagcgagcc
    30541 gttgcccggc tgcgacagac ccagcgcttc ggtcaggcag ttcattgagt tagcggtaaa
    30601 catcccggag caggagccgc aggtcggaca cgcggaacgt tcaacctgat cgctctggga
    30661 gtcagaaact ttcgggtctg cgccctggat catcgcatca accagatcga gtttgattat
    30721 ctgatcggac agtttggttt tcccggcctc catcgggccg ccggaaacaa agatcaccgg
    30781 aatattcagg cgcagggaag ccatcagcat ccccggggtg attttgtcgc agttagagat
    30841 gcagaccatg gcgtcggcgc agtgggcgtt gaccatatac tcaacggaat cagcgatcag
    30901 ttcgcgagat ggcagtgaat aaagcatccc cccgtggccc atggcaatcc catcatccac
    30961 cgcaatggtg ttgaactctt tggcaacgcc gccagccgct tcaatttgtt cggcgaccag
    31021 tttaccgaga tcgcgcagat ggacgtgacc cggtacaaat tgggtgaacg agttcacaac
    31081 cgcgataatc ggcttgccaa aatcggcgtc ggtcattccg gtggcgcgcc acagcgcacg
    31141 agcacccgcc atattacgac catgagtggt ggtggcggaa cggtacttag gcatacttta
    31201 tttactccca gtgtctgtct cgtaaatggg acggtgcgtg ccgtcccatt ttttgtattt
    31261 attgattaac ttgatctaac cagccccatt tatcttcggt ttcgccagtg aagaggccga
    31321 agaaggcttg ctgaatgcgt ttggtaaccg ggccacaacg gccttcgcca acctgaatac
    31381 catctacact gcgcactggc gtgatttctg ccgcagtacc ggacataaac acttcatccg
    31441 ccaggtacag ggattcgcgc gacagaacct gctcacgcac ttcaattccc agctctttcg
    31501 ccagtttgat gatggcatca cgggtaatac ccggcagcgc ggaggaggtg aacggtgggg
    31561 tgaacagcac accatctttc acttcaaaca ggttttcgcc tgcgccttca gagatataac
    31621 cgttcacatc cagcgcgata ccttcctgat aaccgtggcg gcgcgcttcg ctacccacca
    31681 gcagggaaga gagatagtta ccaccggctt ttgccgcggt tgggatggtg tttggtgctg
    31741 cgcggttcca ggaggaaacc atcgcatcga tcccctgctc cagcgcttct gcgcccagat
    31801 acgctcccca cgggaaagcg gcgataatca catcggttga gtatcccgct ggcgggttta
    31861 cacccatgcc aacatcacca acgaagatca gcggacggat ataggcgctg gtgagattgt
    31921 ttttgcggat cacgtcacga caagcttcca tcagctcatc aatgctctgc gaaaccggga
    31981 agcgatagat tttggcggag tcatgcagac gctgcatatg ctcacgatgg cggaatacaa
    32041 ccggtccttt gtgcgagtcg tagcaacgga tgccttcaaa aaccgaagtg ccatagtgca
    32101 gcgcgtgcga catcacatgc accttcgcgt cttcccagcg aaccatctcc ccattgaacc
    32161 aaatgtaatc agctttcttc gtggtcattt ttatattcct tttgcgctca ggcgcggatt
    32221 tgttgtgatg tggttgtgct ctggcagatg gcaacgtgtg cgacgtccac cagtttattt
    32281 aactgactaa acagtaagtc gaccgaccgt gggctggcaa cggtcaattc gatatttata
    32341 ttttgtgcat cgctggcggc agccatattc attgagcaga cgtggaaacc acgatgacgc
    32401 accacgcgta aaacacgttc taaggtttcc ggattgaagc gagccgatac attgacctga
    32461 tgttgcatca tgataatttc tccaacattt ctgaattact ggcgccaggc ggcaccagcg
    32521 gccagacgtt ctcaagttcg tcgattgaga catgaagcag gtatggccca tcactgttca
    32581 gcatggtgtc gagtgccgct tcaacctggt ctttacgggt gatgtgttgg ccagggatgc
    32641 cgaaggcgct ggctaacatg aggaaatcgg ggttatcagt aagggtggtt tcgctgtatc
    32701 gttcctgaaa aaacagttgc tgccattgtc gaaccatccc taaccgttgg ttatcgagta
    32761 agacgatttt caacggtaac tgcttgcgtt ttacggtgcc cagctcttgc acattcatca
    32821 tgaaagagcc gtcaccggag atacagacga cagtatcgtt cggtcgtgcg acttgtgcgc
    32881 caaccgccgc tggtaaaccg aaacccatgg tgcctaagcc gctggaggta atgaaatttt
    32941 ccgggcgagt gtgtgcgatg tgctgcgcgg cccacatctg gtgctgcccc acatctgtgg
    33001 tcacgacgca atccgcaggt ttacgatccg acagttgttt taacaacaac ggcgcgtaga
    33061 tagcgtcacc gggatggtcg taacgccagg tatgttcatc acgcagctgc gcgcagtatt
    33121 gctgccagtc attgatattt aacggctgct gtaatgctgg taacagagca tttaaatcac
    33181 cttgtaatgc cacatgtgcc tgacgcagct tgttcatttc tgccgggtcg atatccatat
    33241 ggataacact ggcgtgtggt gcgaaggtgt tcagtttgcc ggtcacccgg tcatcaaaac
    33301 gtgcacccac ggcgatcagc aagtcgcact cctgcaccgc gaagttcgcc gctttggtgc
    33361 catgcattcc cagcatgccc aggtagtacg gataatctgc ttcaactgcg cccagccctt
    33421 tcagcgtgca ggtggcaggc atttttgtgg tagcgagaaa ttctcgtaaa gcaggaactg
    33481 cctgcgccat acccacgcca ccaccaacgt acagcatcgg tttttgcgct tttgccagca
    33541 tctggcgcgc ttgctcaact tcggcatgtg ggaaagtcac ttcgttttca acggtggtga
    33601 accacggttc caggtcaccg ctggctagct ggatatcttt tgggatatcg accagaaccg
    33661 gaccaggacg acctgagctg gcaacgtcga atgcttcagc cataatgcgc ggcaactctt
    33721 ccagcgactg caccagaaag ctgtgcttgg tacaggctaa cgacaatccc aggacatcca
    33781 cttcctgaaa tgcgtccgtg ccgataaacg gtgcggacac ttgaccggtg atggcaacaa
    33841 cagggataga atctaacagt gcgtccgcaa gcccggttat caggttggtt gcgcccggac
    33901 cagacgtggc gatacatacg ccagttttgc cggtagcacg ggcataaccg atagccgcca
    33961 ttgccgcacc ctgctcatgt cggcacagca agtgctccac gccgccgtca tacaatgcat
    34021 cgtaaaccgg cataattgcg ccacccggat aaccgaaaac ggtgttcaca ccctgtgccc
    34081 gcaacgcatg taccacccac tgtgcgccat tcatagttag ttccccgtcc tgaatcttga
    34141 gaaacagaat tttgtgctgt tattcattgt ctgctcctcg gttatatttt taaggtcaaa
    34201 aaaaaccccc ggacctttcg gtgcgggggt cttagttcgt taaggcttga tctctaagcc
    34261 tttcctcgtc caagtgcagc cccgcacggt gggataataa tcaccaccac gctaatcatg
    34321 accaggctaa tcactcgtag aagggctgtc attttgtctt ttcttgcatc ttgttcgaag
    34381 gaatgcctaa agagttacca tagattttgc gaatagcaca agatattttt tggccgattt
    34441 ttatgatgcg tttaataatt tcaatagaaa ttgttgtttt ttaaaaagaa aatagtgagt
    34501 gaaaaaaatt caatttcagt atgaatgttg ttcccttcct cgtagttcat cctttgtccg
    34561 ctgcattttt gcgagcatca ttccaccgcc agaacaactc aacttcactt gcacaaataa
    34621 acaggaatca gccactaaaa ttcacgaatt agcgggctac agcagcgata tcttatgcac
    34681 ataatagtga ctcaaggagg gcttatgtca ctgtcaattg ttcatacccg cgcagccctg
    34741 ggagtaaatg cgcccccaat cactgttgag gtacatatca gtaaaggtct acccggctta
    34801 acgatggtgg gcttaccaga aacaactgta aaagaagctc gcgatcgcgt gcgcagcgcc
    34861 attatcaata gcggatatga atatccggcg aaaaaaatca ccatcaacct ggctccagcc
    34921 gatctgccaa aagaaggggg acgatatgat ttacctatcg ccattgcgtt gctggcggcc
    34981 tcagaacagc ttacagccaa taagttagat gaatatgaat tagtcggaga actggcgctt
    35041 acaggcgctc tgcgtggcgt tcccggcgca atctccagtg caactgaagc tattaagtcg
    35101 ggcagaaaaa ttatcgtcgc gaaagataac gaagatgaag tggggctaat taacggtgaa
    35161 ggatgcctga tagccgatca tctgcaggct gtctgtgcgt ttctggaagg taagcacgct
    35221 ctcgaacgcc cgaaaccaac tgatgcagta tcccgggcgc tacaacatga tctcagtgat
    35281 gttgtcggtc aggagcaagg aaagcgagga ctggaaatta ccgccgctgg cgggcacaac
    35341 cttttactga ttgggccgcc gggaacaggt aaaacaatgc tcgccagccg tattaatggc
    35401 ctgttgccag atttaagcaa tgaagaggca ctggagagcg ctgcgatatt aagtctggta
    35461 aatgctgaat cagtacaaaa acaatggcgg cagcgcccgt tccgctcacc gcatcacagt
    35521 gcgtcgttaa ctgcgatggt gggcggtggt gcaattccag ggccaggtga aatttcgttg
    35581 gcgcataacg gcgtgctttt tcttgatgag ctacctgaat ttgaacggcg tacactggat
    35641 gccttgcgag agccgattga atccgggcag atccatcttt cacgcacacg agccaaaata
    35701 acctatccag cccgtttcca gcttgttgcg gcgatgaatc ccagccctac cggacattat
    35761 cagggaaacc ataaccgctg cacgccagaa cagacattac gttatctcaa ccggctctcg
    35821 gggccctttc tcgaccgctt cgatctctca ctggagatcc cattaccacc ccccggcatt
    35881 ttgagtaaaa cggtagtgcc gggagaaagc agcgccaccg ttaaacaacg cgtaatggcc
    35941 gccagagagc gccaatttaa gcggcagaat aaactgaatg cctggctgga tagtccggaa
    36001 atacgccaat tctgcaaact tgagagcgaa gatgcgatgt ggttggaagg aacactgatc
    36061 catctggggt tatcgattcg tgcctggcag cggttattga aagttgcacg aaccattgct
    36121 gatattgatc agtctgacat tatcacacgt cagcatttgc aggaggcagt tagctatcgt
    36181 gcgattgacc gtttgctcat ccatctgcaa aaactactga cataaaaaaa gggcatttcg
    36241 ccctttttat taatcatcag aatcggtgta gtcttcagca ccttcaacct gcggtttacc
    36301 gccggaaagg gtgtgaaaac gttttggacg cttaatacgc gtcatatact tggaccacac
    36361 gcgttctgct tctgtcactg gctcacgttc gccacggcat actgctacga agagtttctc
    36421 ttcctcggta accggctcgc gtttgccaag atccaactca ttgaaggcat aaccatgacg
    36481 ctcaagcagt tgtgcctctt tgatggtgaa atcaccatga cgagagaatc cacgtggata
    36541 atgtttattg tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc tgcacgctcc
    36601 taattctttg accgagctag ttatggcgcg gagtattagt tacgcttgac agagtgtaaa
    36661 acaaaacatt taaatcataa cgacaaataa ttttgcggag agcactgtgg atacggaatt
    36721 gttaaaaact ttcctggaag ttagccgaac gcgtcacttt ggtcgagcgg ctgaatcgct
    36781 ctatctgacc cagtcagcag tgagctttcg aatcagacaa ctggaaaatc aactgggtgt
    36841 gaaccttttc acccgccaca gaaacaatat ccgtttaacc gctgccggtg aaaaactact
    36901 gccttatgca gaaacgctca tgagcacctg gcaggccgcc cgcaaggagg tggcgcatac
    36961 ctcacgacat aacgagtttt ctatcggtgc cagcgcctca ttgtgggaat gtatgcttaa
    37021 tcagtggctg ggacgtttgt atcaaaatca ggatgcccat acaggcttac agttcgaagc
    37081 gcgaattgcc caacggcagt ctctggtaaa acagctgcat gaacgccagc ttgatcttct
    37141 tattaccact gaagcgccca aaatggacga atttagtagt cagttgctgg gatatttcac
    37201 tttagcgctt tataccagtg ccccttcaaa actaaaggga gatcttaatt atctgcgact
    37261 tgagtggggg ccagattttc aacagcatga ggcaggtttg atcggtgctg acgaagtgcc
    37321 cattctgaca accagttctg ctgaactggc acagcaacag attgcgatgc ttaatggttg
    37381 cacctggcta cccgtcagct gggcgcgtaa aaaaggcggc ctgcataccg ttgtcgatag
    37441 cacaacactt tcacggccgc tttatgccat atggctgcaa aatagcgata aaaatacgtt
    37501 gattcgcgat cttttgaaaa ttaacgtgct ggatgaagtg tattaatatg aatggctggc
    37561 aaggatgccg gtagaaggat ttacttcgga gagggttatt tcagataaaa aaaatcctta
    37621 gctttcgcta aggatgattt ctggcagggg cggagagact cgaactccca acacccggtt
    37681 ttggagaccg gtgctctacc aattgaacta cgcccctaat tagggtggcg gaacggacgg
    37741 gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg aactaccgct
    37801 ccaccgaatt cttttacaac caccggtttt atgaccggct tactgcttaa tttgatgcct
    37861 ggcagttccc tactctcgca tggggagacc ccacactacc atcggcgcta cggcgtttca
    37921 cttctgagtt cggcatgggg tcaggtggga ccaccgcgct acggccgcca ggcaaattct
    37981 gttttatcag accgcttctg cgttctgatt taatctgtat caggctgaaa atctttctct
    38041 catccgccaa aaca
//
LOCUS       DAZFWL020000035        37129 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFWL020000035 DAZFWL020000000
VERSION     DAZFWL020000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 37129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.035"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            92..754
                     /gene="acrS"
                     /locus_tag="ACHKNP_RS09355"
                     /old_locus_tag="pgaptmp_001871"
     CDS             92..754
                     /gene="acrS"
                     /locus_tag="ACHKNP_RS09355"
                     /old_locus_tag="pgaptmp_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HGU4704045.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(757..936)
                     /gene="yhdU"
                     /locus_tag="ACHKNP_RS09360"
                     /old_locus_tag="pgaptmp_001872"
     CDS             complement(757..936)
                     /gene="yhdU"
                     /locus_tag="ACHKNP_RS09360"
                     /old_locus_tag="pgaptmp_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HGU4704046.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(1020..1904)
                     /gene="yhdJ"
                     /locus_tag="ACHKNP_RS09365"
                     /old_locus_tag="pgaptmp_001873"
     CDS             complement(1020..1904)
                     /gene="yhdJ"
                     /locus_tag="ACHKNP_RS09365"
                     /old_locus_tag="pgaptmp_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU4704047.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1990..2286)
                     /gene="fis"
                     /locus_tag="ACHKNP_RS09370"
                     /old_locus_tag="pgaptmp_001874"
     CDS             complement(1990..2286)
                     /gene="fis"
                     /locus_tag="ACHKNP_RS09370"
                     /old_locus_tag="pgaptmp_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HGU4704048.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2312..3277)
                     /gene="dusB"
                     /locus_tag="ACHKNP_RS09375"
                     /old_locus_tag="pgaptmp_001875"
     CDS             complement(2312..3277)
                     /gene="dusB"
                     /locus_tag="ACHKNP_RS09375"
                     /old_locus_tag="pgaptmp_001875"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGU4704049.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3606..4487)
                     /gene="prmA"
                     /locus_tag="ACHKNP_RS09380"
                     /old_locus_tag="pgaptmp_001876"
     CDS             complement(3606..4487)
                     /gene="prmA"
                     /locus_tag="ACHKNP_RS09380"
                     /old_locus_tag="pgaptmp_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGU4704050.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4499..5950)
                     /gene="panF"
                     /locus_tag="ACHKNP_RS09385"
                     /old_locus_tag="pgaptmp_001877"
     CDS             complement(4499..5950)
                     /gene="panF"
                     /locus_tag="ACHKNP_RS09385"
                     /old_locus_tag="pgaptmp_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HGU4704051.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5940..6182)
                     /gene="yhdT"
                     /locus_tag="ACHKNP_RS09390"
                     /old_locus_tag="pgaptmp_001878"
     CDS             complement(5940..6182)
                     /gene="yhdT"
                     /locus_tag="ACHKNP_RS09390"
                     /old_locus_tag="pgaptmp_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HGU4704052.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6275..7210)
                     /locus_tag="ACHKNP_RS09395"
                     /old_locus_tag="pgaptmp_001879"
     CDS             complement(6275..7210)
                     /locus_tag="ACHKNP_RS09395"
                     /old_locus_tag="pgaptmp_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU4704053.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            complement(7242..8072)
                     /locus_tag="ACHKNP_RS09400"
                     /old_locus_tag="pgaptmp_001880"
     CDS             complement(7242..8072)
                     /locus_tag="ACHKNP_RS09400"
                     /old_locus_tag="pgaptmp_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4704054.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            8414..9268
                     /locus_tag="ACHKNP_RS09405"
                     /old_locus_tag="pgaptmp_001881"
     CDS             8414..9268
                     /locus_tag="ACHKNP_RS09405"
                     /old_locus_tag="pgaptmp_001881"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HGU4704055.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            9304..10194
                     /locus_tag="ACHKNP_RS09410"
                     /old_locus_tag="pgaptmp_001882"
     CDS             9304..10194
                     /locus_tag="ACHKNP_RS09410"
                     /old_locus_tag="pgaptmp_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU4704056.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            10255..11754
                     /locus_tag="ACHKNP_RS09415"
                     /old_locus_tag="pgaptmp_001883"
     CDS             10255..11754
                     /locus_tag="ACHKNP_RS09415"
                     /old_locus_tag="pgaptmp_001883"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU4704057.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            11755..12744
                     /locus_tag="ACHKNP_RS09420"
                     /old_locus_tag="pgaptmp_001884"
     CDS             11755..12744
                     /locus_tag="ACHKNP_RS09420"
                     /old_locus_tag="pgaptmp_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4704058.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFILLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDVFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRRYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            12766..13728
                     /locus_tag="ACHKNP_RS09425"
                     /old_locus_tag="pgaptmp_001885"
     CDS             12766..13728
                     /locus_tag="ACHKNP_RS09425"
                     /old_locus_tag="pgaptmp_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU4704059.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            13725..14771
                     /locus_tag="ACHKNP_RS09430"
                     /old_locus_tag="pgaptmp_001886"
     CDS             13725..14771
                     /locus_tag="ACHKNP_RS09430"
                     /old_locus_tag="pgaptmp_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HGU4704060.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGIVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDGIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVLRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEMSALKAEIAGYR"
     gene            complement(14863..16212)
                     /gene="accC"
                     /locus_tag="ACHKNP_RS09435"
                     /old_locus_tag="pgaptmp_001887"
     CDS             complement(14863..16212)
                     /gene="accC"
                     /locus_tag="ACHKNP_RS09435"
                     /old_locus_tag="pgaptmp_001887"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGU4704061.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(16223..16693)
                     /gene="accB"
                     /locus_tag="ACHKNP_RS09440"
                     /old_locus_tag="pgaptmp_001888"
     CDS             complement(16223..16693)
                     /gene="accB"
                     /locus_tag="ACHKNP_RS09440"
                     /old_locus_tag="pgaptmp_001888"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGU4704062.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPIMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(17559..18389)
                     /locus_tag="ACHKNP_RS09445"
                     /old_locus_tag="pgaptmp_001889"
     CDS             complement(17559..18389)
                     /locus_tag="ACHKNP_RS09445"
                     /old_locus_tag="pgaptmp_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704063.1"
                     /translation="MTSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTKQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(18426..19400)
                     /gene="yhdH"
                     /locus_tag="ACHKNP_RS09450"
                     /old_locus_tag="pgaptmp_001890"
     CDS             complement(18426..19400)
                     /gene="yhdH"
                     /locus_tag="ACHKNP_RS09450"
                     /old_locus_tag="pgaptmp_001890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HGU4704064.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            19552..21492
                     /gene="csrD"
                     /locus_tag="ACHKNP_RS09455"
                     /old_locus_tag="pgaptmp_001891"
     CDS             19552..21492
                     /gene="csrD"
                     /locus_tag="ACHKNP_RS09455"
                     /old_locus_tag="pgaptmp_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HGU4704065.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            21797..22840
                     /gene="mreB"
                     /locus_tag="ACHKNP_RS09460"
                     /old_locus_tag="pgaptmp_001892"
     CDS             21797..22840
                     /gene="mreB"
                     /locus_tag="ACHKNP_RS09460"
                     /old_locus_tag="pgaptmp_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HGU4704066.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            22906..24009
                     /gene="mreC"
                     /locus_tag="ACHKNP_RS09465"
                     /old_locus_tag="pgaptmp_001893"
     CDS             22906..24009
                     /gene="mreC"
                     /locus_tag="ACHKNP_RS09465"
                     /old_locus_tag="pgaptmp_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGU4704067.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            24009..24497
                     /gene="mreD"
                     /locus_tag="ACHKNP_RS09470"
                     /old_locus_tag="pgaptmp_001894"
     CDS             24009..24497
                     /gene="mreD"
                     /locus_tag="ACHKNP_RS09470"
                     /old_locus_tag="pgaptmp_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGU4704068.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            24506..25099
                     /gene="yhdE"
                     /locus_tag="ACHKNP_RS09475"
                     /old_locus_tag="pgaptmp_001895"
     CDS             24506..25099
                     /gene="yhdE"
                     /locus_tag="ACHKNP_RS09475"
                     /old_locus_tag="pgaptmp_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HGU4704069.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            25089..26558
                     /gene="rng"
                     /locus_tag="ACHKNP_RS09480"
                     /old_locus_tag="pgaptmp_001896"
     CDS             25089..26558
                     /gene="rng"
                     /locus_tag="ACHKNP_RS09480"
                     /old_locus_tag="pgaptmp_001896"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HGU4704070.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            26626..30426
                     /gene="yhdP"
                     /locus_tag="ACHKNP_RS09485"
                     /old_locus_tag="pgaptmp_001897"
     CDS             26626..30426
                     /gene="yhdP"
                     /locus_tag="ACHKNP_RS09485"
                     /old_locus_tag="pgaptmp_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HGU4704071.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            30582..32027
                     /gene="tldD"
                     /locus_tag="ACHKNP_RS09490"
                     /old_locus_tag="pgaptmp_001898"
     CDS             30582..32027
                     /gene="tldD"
                     /locus_tag="ACHKNP_RS09490"
                     /old_locus_tag="pgaptmp_001898"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HGU4704072.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNCR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(32155..33084)
                     /gene="aaeR"
                     /locus_tag="ACHKNP_RS09495"
                     /old_locus_tag="pgaptmp_001899"
     CDS             complement(32155..33084)
                     /gene="aaeR"
                     /locus_tag="ACHKNP_RS09495"
                     /old_locus_tag="pgaptmp_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HGU4704073.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            33267..33470
                     /gene="aaeX"
                     /locus_tag="ACHKNP_RS09500"
                     /old_locus_tag="pgaptmp_001900"
     CDS             33267..33470
                     /gene="aaeX"
                     /locus_tag="ACHKNP_RS09500"
                     /old_locus_tag="pgaptmp_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HGU4704074.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            33478..34410
                     /gene="aaeA"
                     /locus_tag="ACHKNP_RS09505"
                     /old_locus_tag="pgaptmp_001901"
     CDS             33478..34410
                     /gene="aaeA"
                     /locus_tag="ACHKNP_RS09505"
                     /old_locus_tag="pgaptmp_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HGU4704075.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            34416..36383
                     /gene="aaeB"
                     /locus_tag="ACHKNP_RS09510"
                     /old_locus_tag="pgaptmp_001902"
     CDS             34416..36383
                     /gene="aaeB"
                     /locus_tag="ACHKNP_RS09510"
                     /old_locus_tag="pgaptmp_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HGU4704076.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     SMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            36475..36747
                     /gene="yhcO"
                     /locus_tag="ACHKNP_RS09515"
                     /old_locus_tag="pgaptmp_001903"
     CDS             36475..36747
                     /gene="yhcO"
                     /locus_tag="ACHKNP_RS09515"
                     /old_locus_tag="pgaptmp_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HGU4704077.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(36803..37066)
                     /gene="yhcN"
                     /locus_tag="ACHKNP_RS09520"
                     /old_locus_tag="pgaptmp_001904"
     CDS             complement(36803..37066)
                     /gene="yhcN"
                     /locus_tag="ACHKNP_RS09520"
                     /old_locus_tag="pgaptmp_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HGU4704078.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         9132 a         8945 c         9736 g         9316 t
ORIGIN      
        1 gcctctcgta taaatacaca ttaggtgata gattaacctt cgctattttc tcactctgtg
       61 tcgaatatat ttatttcctg aataattaat catggcaaaa agaaccaaag ccgaagctct
      121 gaagacccgg caagaactga ttgaaactgc catcgcccag tttgcgcagc atggcgtaag
      181 caagacgaca ctcaacgaca ttgccgacgc tgctaacgtt acgcgtggcg ctatctactg
      241 gcacttcgaa aacaagactc aactgtttaa tgagatgtgg ttgcaacagc cttcattgcg
      301 ggagttaatc caggaacact tgacggctgg attagagcat gacccgtttc aacaattgcg
      361 tgaaaaattg attgtcggct tgcaatatat tgccaaaatt ccccgccagc aggcgttgct
      421 gaaaatctta tatcacaaat gtgaatttaa tgatgagatg ctggccgagg gagtgatacg
      481 cgaaaagatg ggcttcaatc cgcagactct ccgcgaagta ttgcaggcgt gtcagcaaca
      541 aggttgtgta gcaaataacc tcgatttaga tgttgtgatg attattatta atggtgcctt
      601 cagcggaatt gttcaaaact ggttaatgaa tatggcgggt tatgatcttt ataaacaagc
      661 ccccgctctg gtcgataacg tattaagaat gttcatgcca gatgaaaaca taacgaaatt
      721 aattcatcaa acgaatgaat taagtgtcat gtaaattcac attctgtcct gaaaattcag
      781 ttcgtcacag ttaaccagtt ttagtggatt aactgagttc atattgcggc atttgtctgt
      841 ttctgttgcc agcagttcaa tccactgcat cagtaaagta tcaaacagaa aaacgaagac
      901 agcgaaaacg acgagccacc aatacttgcg aatcattacc tgagcctgtg tatgtttaac
      961 caagtctggc aggaaatata cacgaaatga ataatctgaa aaccaggctt acacttcctt
     1021 tactttgcaa tgagatcggg gtcaacttcg cttaaccggc ttcgttttga caggttgccg
     1081 gtctttcttt ttttcacttt cgccagttct tccgcagagt aatgcgacgc gacatccagc
     1141 cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa taccaatgaa ttttcgcccg
     1201 ctggagacgg ctacggcacc ggtagtaaag ctaccggcaa acgggtcgag aacgatatcg
     1261 cctgggttgg aagaggcgag aataatgcgt ttcagtaagg cttccggttt ttgcgtcggg
     1321 tggttctcat attcatccat caaataacgc acgcgcggaa aatcccagac gttacccggt
     1381 actttttgat gattgtatgg ctgtggagga tttttgcgat aatcgatcaa cgcgcgctgc
     1441 gatccggttt tggcttctac cagaatagca tcaccgttga atgtgtagtt ctttgcgtct
     1501 ttcaccatca tcaggatggg ttcgtacatg gagccgtagt gttttttcgc ctgtactcca
     1561 gaactgtcat atgaccagac gatgcgactt ttgatggtaa aaagcttgcg gcactggaga
     1621 tcgataaagg gcatgttttc cgtgctgttc atgatgtaca tgctgccctg ctttttcaga
     1681 acgcggtggc actctgcaat cacttcaaac agccagtcga taaacagatc ttctttccag
     1741 gcttcgatca gaccatcaaa atttttaccg atgttatatg gtgggtcggc aaagatcaga
     1801 tcgacacttt cggcggggat ctttttaagt tcggcaagcg catcaccgtg aataatggtc
     1861 ttagcttcat taccaaaccg ggtcggttca catcctgttc tcatggtcac tccctttgtg
     1921 acacctataa aaaaggcgct tccccatgcc gagtagcgcc tttttaatca agcatttagc
     1981 taacctgaat tagttcatgc cgtatttttt caatttttta cgcagcgtac cacggttgat
     2041 gcccatcatc agcgcagcac gggtctggtt accacgggtg tattgcatca ccatgtccaa
     2101 caggggctgt tctacttcag ccagtaccag ctcatagagg tcattcacat cctgaccatt
     2161 cagttgagca aaatagttct tcagtgcctg tttaaccgag tcacgcaggg gtttttgggt
     2221 tacctgatcc tgagagttaa cggtagaaac ggtcagtacg tcagaattta cgcgttgttc
     2281 gaacatagtt ctgtcagctc tttatttctg tttacgcaaa attttcgaag tatgcctcca
     2341 acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa tgtgcgccga aactggtcat
     2401 ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg tgcaattcgg tacccttttg
     2461 ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca aagcaagcgc ttaacctctg
     2521 ccaaaggcag cgggggcagc aactccccag tgtccagata atgctggatt tcccgaaaga
     2581 tccagggtct tccctgagct gcgcggccta tcatcagggc atccgcccct gtatagtcga
     2641 gcacagctct ggctttaagc gggtcagtaa tgtcgccatt cgcgataacc ggaatggaaa
     2701 ctttctgctt aactgcccga atactgtcgt actcagcttc tccattgaac aaacaggcgc
     2761 gtgtacggcc atgaatggtc agagcctgaa tgccacagtc ttcagccagt tgggcaatct
     2821 cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat cttcagggta acaggaacgt
     2881 ccactgcatt gacgacctcg gtaaggatcg atttaacgac atccgggtac tgcaagaggg
     2941 ctgaacctgc gagcttgcga ttcacttttt tagccgggca acccatattg atatcaataa
     3001 tctgggcacc gctttccacg ttaatacgtg ctgcatctgc catttctttc ggatcgctac
     3061 cagcaatttg cacggtgcga ataccgggtt catcaatgtg caccatccgt aaacgagatt
     3121 tgtcgctttc ccaaacctgt gggttagaag acatcatctc ggatactgtc aatccggctc
     3181 ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat gccagccatg ggcgctgcga
     3241 tcaggcgatt tctgagctga tattgtccga tgcgcatgag ttaagaaatg accatactgt
     3301 gactgcaagg cggcgtatat tacgcatttt ttgcacgaga tgaaaggcca aactttgacc
     3361 aatcctctga gatggatcaa agaattgcat ttaaaatgag cgtggtgcga taattactca
     3421 taaaaatcat catattagaa aatagtgact aaaatttaca ctcaaagaaa tttgagtaag
     3481 ttctcaattt ttctttatga atgaaaattt tggcacgcaa atttgcgtaa ataaccggca
     3541 aatttacgtg ccttttgtga gcttgctcgc acttcgcccc gcgtcaccct acggcgatgc
     3601 gaaggttaat tcttacgacc ggtaatacgg caccactctt ctttttccac gaccgggtcc
     3661 agtgcgaagc tatcggcata agcttcacaa acgctctctg cctggcttgc cagaataccg
     3721 gaaaggccca gcaaaccgcc tgaaaccggc aggacgctga ttaacggtgc cagttcacgt
     3781 aatgggcctg caaggatgtt agcgaccacc acgtcggctt tcatttcttc tggctgatct
     3841 ttcggtaagt agagttccag acggtcagaa acgccattac gttcggcgtt gtcgcggctg
     3901 gcctgaatcg cctgcggatc gatatcaata ccaatggctt ttgctgcacc cagtttcagc
     3961 gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga tgactgtttt accggttaaa
     4021 tcgaggctgt cgagccattg caggcacaga gaggtggttg gatgggtacc cgtaccaaac
     4081 gccagccctg gatctaacat cacgttgacg gcgttttcgt ccggcacatc acgccagcta
     4141 gggcagatcc acagtcgttc accaaagcgc atcgggtgga aattatccat ccattcgcgc
     4201 tcccagtctt tatcttctag ttgttcgatt ttatgcgcga agcctgcgcc gagcagcgga
     4261 tggttttcca gaatcgccac cacgtcgttc atatcggttt cagcgtcgaa cagaccaatc
     4321 acatcggtgt cgccccacag gcgcgtttcg cccggcagcg gctcaaatac tggcgtatcg
     4381 tgggtatcct gaaaagtgat agaaacggca cccgcttcca tcagcgcatc gctaagatct
     4441 tccgcgttcg cgccggtggt gttcagtttc agttggatcc aaggcatggc aaaactcttt
     4501 atttatcagt agtcaaaacg gtagcttgcg ggacggatgt accgaaacgg tttccgacca
     4561 ggaaagccag caaacttagt agtaacgagg gcacaatagg gtggaagccc aggtactgaa
     4621 tattcagcgt cgcgagtacg gcatacagca cgccgccaac gatcatcgca cttagcgcgc
     4681 ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag cggccacagg aaaacggctt
     4741 ccagcccacc gaaggccagc aaattcagcc agatgatcat ttctggcggc ttccaggcgg
     4801 caagcagcag caacgcgccg agaactaacg taattaccgc cgacatccgc ttcagacgcg
     4861 tctcgttttg catttgatcc ggacggatat tcagatagag atctttaatg atcgtagcgg
     4921 aactttgcag caactgggca ttgatcgtcg acatgatcgc agccataggt gcagccagga
     4981 agattccggc agcaaacggt ggcagcactt ttaccattaa cgtcgggatc accagatccg
     5041 gtacggtgag atcggggatc actgcacgac ctaacgctcc ggccaggtgc ataccgaaca
     5101 tcaggattgc gactacaatc gtaccgatga tgatcccccg gtgtactgct ttgctgtctt
     5161 tataagagat acagcgcacc gcagtatgcg gcaggccaat cacgccaaag cacaccagta
     5221 cccagaacga cgtcataaag gcaggcgaca gaatatcgtc agcgccttgt ggcgtaacca
     5281 gttgcggatc gatggtttgc aaggtctgta ctgcgttgct taagccgcca gcggcatgta
     5341 ccacgccaat aagcagcaca acggtgccaa tcagcatcac aagcccttgc atggtgtcgt
     5401 tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa cgcaatgctg ataccaaaaa
     5461 tcagcagccc ggtttcataa ggaatacccg ccgcggtttc cagcaggcgc gcaccgccga
     5521 taaactgcac ggtcatcgca ccaacgaacg caaccagcaa actcaaactc gccagccaca
     5581 ccagaagacg actctggtag cgggcaaaca gcatatcgtt cagcgtcact gcattgtagc
     5641 ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag tgaaagccag actgcaggaa
     5701 gctgaatcat cgccagcaat acccagccca gcccgtattt ataagcagct cctggcccgc
     5761 cgataaacga actggcactg atataggtcg cggtgagcgt catcgccagc acaataccgc
     5821 ccatagagcg gctgccgagg aaatactcat taaggaaggt gccggtgctc cgtttacgca
     5881 tcgcataaac cgagataccg aacaccacca ccagataggc gaccagcggt agaattactt
     5941 caagctgcat cgtcatcctc cagtgggata tcgcgataga taaatttcac catcgcccag
     6001 cacagtccaa taaacagcag cggcgtcagg atgcaggcca tctcaaacca gcgcggaaag
     6061 ccggtaaaac cgggggcaac gccaggtaag taagcggcta ctaaccaaac tgccagatac
     6121 aaaagggtca gccccagcgc ccagcgcgcc tctttatggg cctgaacaaa acgagtgtcc
     6181 acggaagggt tcctcctcat ggggggatac gagaaacggg caattctacg gcaactcttc
     6241 aggcagggaa aataatctga cagaaaattg ccgttcagtg ctgctgcaaa aagtcttcaa
     6301 tgtcagccag tgaggatatc ccttccatcg gaccttgccg cattacggcg agcgcgccgc
     6361 tggcatttgc atattgcagt gccttatgtg ccgggaaacc ggataagaaa agagtgataa
     6421 acgttgcgcc aaagcagtcg cctgcacccg ttggatcgat aatttcgata tcgtggcctg
     6481 taacatgctg ggcgtgtaat gtaccgtttt taagcttgta atagctggcc ccacgctggg
     6541 cgcgttttat cgccacatgt tttacgccgc cgtggagaag atcgctaaca atctgttctt
     6601 ctgacagatt tttgtgacgc gcgaagaaag ggagttcgct ttcgctgggg atgaaaatat
     6661 ccgtatattc aatcaaataa tcgagagcct gcgccatttc aggaatgctc agcatctctt
     6721 tgcgaatatt gggatcgaaa gaaacggtgc cgccagccga tttgatcgtc gttattgctt
     6781 tacgcatgac atcgatcatg cgaaatgaga acaacgatga gcccacaata tggagatggt
     6841 tacactgttt tagcagatcc ttatcaatgt gctcagcagt aaacaagccg caggcgctgt
     6901 tagggatatt aaagacgaaa tcgcgctgct ggggattttg ataactcacg aacgccgtac
     6961 ctgtgacggc attgttcata acatggatcc catcggtaat gacaccttca tgacggaggc
     7021 gctctataat gagtcggcca aaatcatcat taccaacaca tccgaagaag atggcccgat
     7081 gggacagttt tgccacctga gcagcaaaaa ttgctggcgc accgctggga aatggcccga
     7141 taaactcccc aggctgagtg aatttttgat gtgggttgcg ggacaaaaac tcggccagca
     7201 attcgcccat agcgcagata gttattgtca caaaacccct ctcattcctg aagcctttcg
     7261 cgattggacc aggtaaagca ctcattactg gtataaagga taacgaaatc agccgactgc
     7321 aattgttgct gatattgttc gttaatgctg cggctgacga tcacattttc tgcctgatgc
     7381 aaatgcccga tagcagccgt tgatggcgca ggatcggcgg caacgataac cgtcttattc
     7441 tgggcatgat cgaaacagag ctgccaggca gcggccagtc tgggaacgtt gatatttaaa
     7501 gtaccatcag catccagatg gctgatttcc agaatgctat gtgtaatggt gaaatgctga
     7561 agtgcctgtt caagcggttt tccggccagt gcggtgtcgg ggcgcagcat ttcactgcca
     7621 gccagtatta tttcgccatc aataaactga tttgccagca gcgcatgttc gggatcgttg
     7681 gtgatcagac gcagtttttt cacttcgctg agcaatggaa tgatcttacg gcagattgtc
     7741 ccatgtccaa gaaatagcgt gtcacgatcg cgaattaacc gtgcgcaatg tcgtgctatt
     7801 tccatttgtt gtgccagaga caatggcgcg atcggttgta tcgcttctgg ttcagaggag
     7861 agtggtgtcg caatagcacc gccaaatgat cgctttaaat atccctgttg ctccagataa
     7921 ctgagatcgc cgcggatcgt cacgccagag acaccaagtt gcgcagataa gtcatcaact
     7981 ttgatttcac cttgggcggc aacaatttct gcaattttta atctgcgttt cagtaggtta
     8041 gacgattttc tcggcacgtt aacactcttc attggccttt aatgcgtaac actatagccc
     8101 cttgtatttt tctttgaaag ttatccttca ataatatcga caattcgaaa agaaaatcat
     8161 gggccaaaaa tcgcaacaaa aatagattaa ttcattgaga aataaatgaa tttattcata
     8221 tttatgcaga gatgttattg gtttcacttg taaagtgaaa aacaaattaa gttatttttt
     8281 gtaagttatt ttgatgatat tcgacatgca tcacgctttt tgccgctttt gctgtgctag
     8341 tttgctttca aaatgaaagt gaatattctc tttcttcatt ttgtaaggcg aacgacaaat
     8401 aacaggagct gcgatgtaca tcatttcaag caaaaacatg ctgaaaaaag cccagtcaga
     8461 gggatatgcc gtccccgctt tcaacattca taaccttgaa actttacagg tagttgttga
     8521 gagcgctgca caacttcgct ctcccgttat gctggcggga acacccggta cttatcgtta
     8581 tggtggcgtg ggaagtttaa tttcgttagt acaatcgctg gctcgcgagt acaacctgcc
     8641 gttagtcctg catctcgacc atcatgaaga gagtgatgac atttttaaca aggttcgcgc
     8701 agggatccgt tctgtcatga ttgatggctc ccatttgcca tttgaagaca acattgcgcg
     8761 agtgcaagaa gttaccacct tttgccatcg ctttgatgtt agcgttgagg ccgagctagg
     8821 gcgtcttggc ggccaggagg acgatctgct ggtggatgaa aaagattccg cttatacctc
     8881 accagccgca gctgcagagt ttgttcaacg tacccaaatt gactccctgg ccgtagcaat
     8941 cggcacagca catggtttat atgcgcatga accacgactg gattttgtgc ggctggaaca
     9001 gattcgtcag cgagtggaga ttccactggt gttgcatggt gcttcgggct tatcagaaca
     9061 ggacgttcag cactgtattc gccgcggtat ttgtaaagtg aatgtcgcga cagaattgaa
     9121 aattgcgttt gccgatgcgt taaaaggata tttctacaac aatcctgcgg caaatgatcc
     9181 acgccactat atgcagcccg caaaggccgc catgaaagaa gtcgtaactc gtattattgg
     9241 tgtctgtggt agtgaaggaa aaatttaaga cgttgtaccc tacaaaaaca atgaggattg
     9301 tttatgaaaa atattaaatt acttggttca gctatgttgg tgagcctggc attgttctcg
     9361 caaaattcgt tggcaaaaga gtataaaatt ggtgcgtcat tattaacaca gcaacatcct
     9421 ttctatattg atcttgcgaa tgccatgaaa gaagaggcaa aaaaagacaa tgtcaattta
     9481 agtgtctcta ttgccaatca ggatttaaac aaacaacttt ctgatgttga ggattttatt
     9541 actaaaaaag tggatgccat tattatctct ccggtcgatt cgaaaggcgt ccaggcggcg
     9601 ataattaaag ccgataaggc cggtattccc gttatcaccg tcgacgtcgc tgcggaaggc
     9661 gttccggtcg tatcgcatgt tgcgaccgat aactatgcgg gaggcgttga agcaggcaag
     9721 ttaatgggca aacttttgaa tggtaaaggt actgtagcca ttatttctta tccggcacta
     9781 caatcggtgg ttgcgcgcgt tgatggtttt aagaaagggt taagcgatac accggatatt
     9841 aaaattgttg ctgaacagcc tggtattacg cgggctgaag cgttaacgac tgcgcaaaac
     9901 attatgcagg caaatgccaa cctgaatggt ctgtttggct ttggtgatga tgcggcgctg
     9961 gcagcggtta tcgcagcaaa atctgcgcat aacgacaaca tcaaaatcat tggttttgac
    10021 ggcatgaaag aagcgcgtga tgccgttgac agtgaaaaaa cgtttgcggc cgtgattcgg
    10081 caatatcccg atcaaatggg tgctaaagcc attgatgccg ctgttgatca cctcaatggt
    10141 aaaccggtag aaaaaatgat cccggtacaa cccggcgttt atacaggtaa atagtaaaca
    10201 tcttcacctc acaacaaaac gttattggcg ttttgttgtg acaccgaggt cattatgacg
    10261 gatgttattc ttgatgtttc gcatattgcg aagacatttg gtcatgttca agcattaaaa
    10321 gatattaccc tctctttacg taaagggcgg gtgcatactt tattaggcga gaatggtgcc
    10381 ggtaaatcga cattaatgaa aatactggct ggtgtttatc cgccaaccca gggaacgata
    10441 acactacgtg gtgaaactat caccattaat aatccgcagc attcacgcca gttaggaatt
    10501 gcgattattt ttcaggaatt gagcctgagt aataatatga ccgtggcgga aaatatttat
    10561 gccaataatg aaccgcgacg ttttggcatt attaacgata aaaaaatgct cgcagattgc
    10621 cagaatctcc tcgcggaact gggcatacca ctagacccac tagaaatggt cggcaatatg
    10681 tcgatggcac ataggcaact ggtagaaatt gctaaagcct taagctatgc agcggatgtc
    10741 gtgattatgg atgaaccgac atcttcgcta agtgataatg aagcggagat tctgtttaac
    10801 attattgaaa aattaaagca gcgaggctgt gcggttattt atatctctca tcgcatggat
    10861 gaaattatgc ggatttcaga tgatatttcg gtgatccgcg acggtgagta tatcgccacg
    10921 catgaaaaga aaaacagtga tatccagcat ttgattgccc aaatggttgg tcgggaaatg
    10981 aaaaatattt ggccagcacg tcttggggaa ataccggatg aaaatgttcc tgcaaaactg
    11041 gaagtaaaaa atctttccca tccttcgctt tttaaagaga tcagtttcgc tgtgcgtccg
    11101 ggggaggtcc tcggattttt tggcctggtg ggggccgggc gttccgatgt gatgaaggcg
    11161 ctctttggcc tcgtttcgta tcaggggact gtgttgattg atggtaagga agtcaggatc
    11221 gctaatccta aacaggctat tgatcatggc attgcttttg tgaccgaaaa ccgcaaggaa
    11281 gaagggttag tgctgatgca cgacgtcaat atgaacacgc accatgtggc gtttcaatat
    11341 aacgcttcgc ggatgggact catcaatcac cgacaagaag aggcgaagac gctgcaatca
    11401 attgccagaa tgaacactaa agtgagttct gtacatcagg cagtgggagc gctgagcggc
    11461 ggtaatcaac agaaaatcgt gttatcgaag tggctggaga aaactccgcg tattttattg
    11521 ctcgatgagc caacgcgcgg agtggatgtc ggcgcaaaat ttgaaatcta taacgtgata
    11581 cgtcaactgg ctgcggcagg gacagcaatt attctggtgt cctctgaact gccagaagtc
    11641 atggcactta gcgatcgtct ggtggtaatg cgtaataaaa cgattgccga tatttattca
    11701 tgtgaaaatt taacgcagac acaggtaatg accgctgcaa caggagtacg ttaaatgaca
    11761 gagaaaactc ttgccagtcc gaaaccttcg gaaattccca ttgcaaataa ttttaaaaaa
    11821 gtcttcaggc aatatggcgg tatcctcagt ggaatggtgg tgttaatcat tctgttcagt
    11881 tttattaatg acagtttttt taccgccaac aacattacta atattattct tcaggtttcg
    11941 ataattgcaa ttaccgccta tggaatgact tatgtacttt tgcttggcga tattgattta
    12001 tcggtcggtt caaccattgc attgattggc acatttgctg cgcttggggc ttcatgggga
    12061 attccattta ttttattggt cccattatcc attattgcag cactggctct ggggatgata
    12121 aatggtggct tgacggctat agcggggatc ccgtcgttta ttgtcactgt tgctactatg
    12181 gggatcttcc gtggtatcgc ttatatagtg accgatggta tgcccatcat gattaaagat
    12241 gatgtctttc tggcactggg gaatggagaa tttctctata tacctattcc aatctggata
    12301 ttaatcatct tattgctgat taaccacttc attcttacta aaacaacctt tggacgaaaa
    12361 atttatatca ctggtggtaa taaagaagcg gcaatttatt cagggataaa tgtcacccgc
    12421 ttgaaaatta aagtctttat gattacagcc gtgctggcag gtatcagcgg gatgatcctc
    12481 gcctcacgcc gctattccgg gcaaccgaat tccgcgttga gctacgaact ggatgctatt
    12541 gctgcggctg tactgggagg caccagcctg aacggcggct atggaaccgt ggtggggacg
    12601 gtgatcggcg cattaactat cggcgttatt aataatggca tgaacttaat gaatgttccc
    12661 tatttttacc agatggtggt taaaggatta gtcattcttg tagccgttta ttttgacgta
    12721 agaaataaaa gaaaacgcag ctaatcattt caaggaatta atcatatgag taaggtcttc
    12781 acaattggag aaattctggt tgaaattatg gcgagtaaaa tcggccagcc atttgatcaa
    12841 ccaggtatct ggaacggccc ttatcccagt ggtgcgcccg ctatttttat cgaccaggtg
    12901 acacgtcttg gcgttccgtg cggcatcatt agctgtgtgg ggaatgatgg ctttggtgat
    12961 attaatatac atcgtctggc tgctgacggt gtggatatca gggggatttc agtcttacct
    13021 ctggaagcta ccgggagtgc ctttgttact tatcataact ctggcgatcg cgattttatt
    13081 tttaatatta agaatgccgc ttgcggtaaa ttatcggcgc agcatgttga tgaaaatatt
    13141 ctgaaggact gtacccattt tcatattatg ggctcttccc tgttttcgtt tcatatggta
    13201 gacgcagtaa aaaaagcggt aactatcgtt aaagcaaatg gtggtgttat ttctttcgac
    13261 cctaatattc gtaaagagat gctcgatatt ccagaaatgc gtgacgccct ccactttgtt
    13321 cttgagttaa cggatatcta tatgcccagc gagggcgaag tgttgctctt gtcgccacat
    13381 tcgacgccgg agcgtgcaat cgcaggtttt cttgaagaag gtgttaaaga ggtgattgtg
    13441 aagcgcggca atcaaggtgc cagttactac tccgcgaatg agcagtttca tgttgagagc
    13501 tatccggtgg aagaagttga cccgaccggt gcgggagact gctttggtgg cgcgtggatt
    13561 gcctgccgcc agctagggtt tgacgcccat cgagcgctgc aatatgccaa tgcctgcggt
    13621 gcgctggcgg taaccagacg tggcccaatg gagggaacgt ctcgccttgc ggaaatcgaa
    13681 gcgttcatcc agcgccatga catgtccatt cgggaagcag cacaatgaaa aacgaaattt
    13741 ggtcgtggat tggcgatctc tcgcaggttg ccggaattaa acactatgag ttacgtagtg
    13801 gcagagcaaa gggtacagaa gcgtttgatg tccgtactgg tgcgggactg gcatttacgg
    13861 tggtaaaaga tcgcgcgctg gatattgcat gggccagcta caaggacacg gcgctttctt
    13921 ttatcacccc caacggcatt gtcgcccccg ctttttttga gtctcagggc aacggatttt
    13981 tacgtagctt ttacgcgggg ttactgacta cctgcggact tagctacatt ggcacgcctt
    14041 gtgaggatga aggagaaacg ctggggttac acgggcgttt ggctgcgact ccagcagaag
    14101 aggtggggta tcgcacagaa cggaccgacg acggcattga atttgtcatc aatggcaaag
    14161 tacgggaaac gcgactgttc ggcgaaaatc tgacgctgga aaggaccatt cgctgccgtt
    14221 atggcgaaaa tgtgctgcgc attgaagaca aggtgactaa tcatggtttc acccgccagc
    14281 cgctgcaaat tctttatcac tttaattacg gctggccttt attatcgcca caggcagaaa
    14341 tcttgctgtc ggctaaaagc gtaacgccac gaactccgca tgcggcggaa gggttaacca
    14401 gtcatctgga gatttgtacc ccacagcccg gttttgacga gcaggtttac tatctcacgt
    14461 tgaatagcga cagtcagggg atgagtaaag ttgctctggt gaacgccgaa ctggggtggg
    14521 gaatttacga gaaattcgac accatgcagc tacccaactt tatccagtgg aaaaatttag
    14581 gcgcgggtga gtatgttatg ggactggagg tcagtaatag ttttcctgac ggtcgtgata
    14641 aagaacgagc gcaggggcgt ctgcccttta ttgagccagg ggaaacaaag aaatactgct
    14701 ttgagctggg aattgttgat ggcgatgctg aaatgagcgc gttgaaagcg gaaattgcag
    14761 gctaccgtta gctaacggca ataaaaacgc cgggcagcgt taacaggccc ggcgttgatt
    14821 acgttctttg ctaagcgcga tgaaaacacc gcgcgtcaca aattattttt cctgaagacc
    14881 gagttttttc tccagatagt ggatgttagt gccaccatgc tggaagttct cgtcattcat
    14941 gatgcggatc tgcagatcaa cgttggtttt gataccgtcg atgatcagct cctgcagcgc
    15001 attcttcatg cgggcaatcg ccacgtcacg gttttcaccg tagcaaatca gcttaccgat
    15061 cattgagtca tagtacggcg gtacggtgta gcccgcgtag atatgagact cccaacgtac
    15121 gccaaaaccg ccaggtgcgt ggaaacgggt gattttgcct gggcttggca ggaaggtgtt
    15181 cggatcttcg gcgttgatac gacattccac cgcatggccg cgaacgtgaa cttcttcttg
    15241 cttgatcgac agcggttgac cggcagcgat acgcagctgt tctttgatca ggtcaacgcc
    15301 ggtgatcatt tctgtaaccg ggtgttctac ctgaatacgg gtgttcattt cgatgaaata
    15361 gaactcgccg ttttcgaaca ggaactcgaa agtacctgca ccgcgatagc cgatatcaac
    15421 acacgcttta gcgcaacgtt cgccgatgta gcgacgcagt tccggggtaa tgcccggtgc
    15481 tggtgcttct tcgaccactt tctggtggcg gcgctgcatg gagcagtcac gttccgccag
    15541 atagatagcg ttgccctgac cgtcagccag tacctgaatc tcgacgtggc gaggattttc
    15601 caggtatttc tccatgtaaa ccatatcgtt gctgaaagca gctttcgctt ccgcacgggt
    15661 catggagatg gattgtgcca gttcagcgtc gccgcgcact acgcgcatac cgcgaccacc
    15721 gccgccgccg gaggctttga taatcaccgg ataaccaatg cgtttagcaa tggcacggtt
    15781 tttatccata tcgtcgccca acgggccgtc agaacccggt acgcaaggga cgcccgcttt
    15841 tttcatcgcc gcgattgcgg atactttgtc gcccatcagg cgaatggttt ctgctttcgg
    15901 gccgatgaag ataaagccgg agcgttcaac ctgctcggca aagttggcgt tctcggagag
    15961 gaagccgtaa cccggatgga ttgctactgc gccggtgatt tcagcggcgc tgatgattgc
    16021 cgggatgttc agataacttt ttactgacgg agcagggcca atacagaccg tttcatctgc
    16081 cagtaatacg tgttttagat cgcgatccgc gctggagtgc acagcgacag tcttgatgcc
    16141 cagttcttta caggcacgaa gaatacgcaa tgcaatctcg ccgcggttgg caataacaat
    16201 tttatccagc atgttcgcct cgttactcga tgacgaccag cggctcgtca aattctaccg
    16261 gttgtccact ttcgaccaga attgctttca cggtaccgga tttgtccgct tcgatctggt
    16321 tcatcatttt catggcttca acgatgcaca gggtatcgcc cacgttgact ttctgaccca
    16381 cttcgatgaa cgcttttgcg tccgggcttg gggtgcggta gaaagtacca accatcgggg
    16441 aacgtacgat gtgaccactg atttccgctg ctgctggcgc ttccatggaa ggaacggtcg
    16501 ccggagcggc tgcgttagat tgagctggct gctgcattat tggtgcagcg taagcttgtt
    16561 gcatcacagg gaaacttgcg gcaggagctg cacggctaat gcgtactgac tcttcgcctt
    16621 cagaaatttc cagttcggag atgcctgatt cttcaaccag ctcgatcagt tttttaatct
    16681 tacgaatatc catgagtggg ttccgtactc tttgtttagt gtgattgtga caggcgtttc
    16741 accgccgtct gtaaagcgta tgccccgaca gctgtatgca tagcgataaa ttccagcagg
    16801 ccgggacgcc ggtctatttt gccccagagt gtcaatgtta gacttgacgg acattgtgca
    16861 gcgatctgct gctatcctcc ggcaaaaaac aaaatatacc ttcgttgcgc caatgtcacc
    16921 ttttccgcag cgcaaaaact gcgtcaggga ggacgaggtc gcacattata acgatttcgt
    16981 agcaattggc agctaaatac tggtcttatc agggaagata atcaacagct aacatgtaaa
    17041 taaccttcaa caccgtgtaa tttgcaacaa gccgcacaat tcacgaaatt agcgccatca
    17101 tcgacggaac ttcttataac gtaaggtgaa aagctgtaaa gccagccctg cgaagatgag
    17161 gggctgcaat gagataatct tcacagacca caaataatgt agggatgcca ggatcgcgac
    17221 aagatagacg acattacgca gcggttgctg gggattaccc aattttcact gcattgtctg
    17281 gatagatgtt aatgctaaag cacgcaaaat taaccgattg acaatgctca gcattaaata
    17341 ggttcgagtg attaatttgt agtcaaacaa aatcagatta ttccccccat tttgcagaag
    17401 gtaaactgtt ttgtcaggcg gcagtgcggc tatctgatgc tttcagaatt tgccacctga
    17461 caataaaaaa gccgatttgg tagcaaaatc ggcttcggtg agtttgcagt tttcatcaca
    17521 tactccgaca agctttttca agcatgacgg aggttgcgtt aatgcaaggt ttctacccag
    17581 ggataaaagt gaccgttacg gcggatgaat atctgtgttg tcagaggatg gtcatcctca
    17641 cggaagcatt gttttgttag ctgtcgtaat gcactgagat ttgctggctg atcattgtgg
    17701 gtgaaaaaca gttttcctct ggagggaacg gtcactaaca ggtcgccgca ttcaggatcg
    17761 ggtgattgca cccaaagatc gtcaatgagc atcagactgg gctcataagt accccctgcg
    17821 gagataacgg caacttttcc atcgtttgcg cctgtatcaa cgatttcagc cgtaacttcc
    17881 ggcaatattt gcttcaaatt actgatggct tgttcgcggt caatggtcac ttcgtagcgc
    17941 atcagatagc gcatggtttc gctaccatct tcagcaaaca agataattaa ctcatcgtta
    18001 aagtcttccc aggcaaaatc cagcgaggcg ttgaggacct ctggatcaga ctcatactgg
    18061 ctaagctgtt ggcgaacggt atccagccag gctttttctt taatcaccgg aacaatacag
    18121 cgcccggtcg gcagcatttc tgcaagctgt ttcagcatat ctgcagccac ttcttgcagc
    18181 gggtctggtg cggcttgata acgtttgtag gcatcatcaa gggtgatttc caccgtttgg
    18241 tgacccgtat caatgcgcat caatttgtgg tcagtcgaaa tcatgacttt ccagtttgga
    18301 tgatgttgta caacttcctg accaaggtgt tcgacgaaag ctgtccggct aaccgctttt
    18361 cccgttaacc gattgaataa tgatgtcaaa atgagttccc tctcttatta ttccttgcta
    18421 aatggttagt taaccttcac cagcgtgcga ccctggatct ggttattaat gatggcctcg
    18481 gcaaagttcg gtgcctctga cagagatatc tcttttgccg cctgggtata gaatgattcc
    18541 ggtaaatcgg cgaccagtcg ctgccaggct tgtgcgcggc gttctggtgg cgtcattact
    18601 gaatccaccc cttgcaaacg gacattacgc agaataaatg gcatgaccgt ggttggcaga
    18661 gtaaaaccac ccgccagacc acaggcagcc acgcagccgc cgtaattcat ttgcgccagc
    18721 acttttgcca gcactttgtc gccaacagtg tcaattgccc cagcccagac ttgtttttcc
    18781 agaggacggg attcggcaaa ctcatcacga gggagaatac gactggcacc taaacttttc
    18841 agatattcat gggtactttc gcgaccggaa acggcaacga cctgataacc caacttatgc
    18901 agcagcgcca cggcggtact gccgacgcca ccactggcac ccgtcacgac aatctccccg
    18961 tcctgcgggc gaacaccggc atcttccagc gccatcacac acagcatggc ggtaaaaccg
    19021 gcagtaccga taatcattgc tttacgcgcg tccagccctt gcggcatggc aaccagccag
    19081 tcacctttca ctcgcgcctg ctccgccagc ccaccccagt ggttttcacc aacgccccag
    19141 ccagtgagta acacctcctg accggcatga aaacgcggat cttcgctggt gcgtacagtt
    19201 ccggcaaaat cgatcccagg aatcatcgga aaattacgga tgatttttcc cttaccggta
    19261 atcgccagcg catctttata gttcaggctc gaccagtgaa catcaaccgt gacatcgccc
    19321 tccggcaggc gactttcgtc aagtgtctgt accgatgcga gagttttgcc gtcctgctgt
    19381 tctaaaagta acgcctgcat aagtggtcct catgtgcatg atggattgga aaataatttc
    19441 tgaagactat actcgctaat ggaaaagcaa agccgatgaa gcgcaataaa ttgcgagata
    19501 aatctgattt gctagtatgc ccgcttcctc actatcggag ttaacacaag gatgagatta
    19561 acgacgaaat tttcggcctt tgttacgctg ctcaccgggt taacaatttt tgtgactttg
    19621 ctgggctgtt cgctaagttt ctacaacgca attcagtata agtttagtca tcgcgttcag
    19681 gcggtggcga cggcgattga tacccacctt gtgtcgaatg acttcagcac attaaggcca
    19741 caaattaccg aattaatgat gtcggcagat atcgttcgtg tagacctgct ccatggtgat
    19801 aaacaggttt ataccctggc cagaaatggt agttatcgtc cggttggctc cagcgatctg
    19861 tttcgcgaac tgagtgttcc gttgataaag catccgggga tgtcgctgcg tctggtttat
    19921 caggatccga tgggcaacta tttccattct ttaatgacta ccgcgccgct cacgggggcg
    19981 attggcttta tcattgttat gctcttcctg gcggtacgct ggttacaacg gcaacttgcc
    20041 gggcaagaat tgctggaaac ccgggctact cgtatcttaa acggtgagcg tggctctaat
    20101 gtgttgggaa ccatctatga atggccgccc agaaccagca gtgcgctaga tacgctgctt
    20161 cgtgaaattc agaacgcacg cgaacaacac agccgtcttg atacgctgat ccgctcttat
    20221 gccgcccagg acgtgaaaac cggcctcaat aaccgactct ttttcgataa tcagttagca
    20281 acgttactgg aagatcagga gaaagtaggt acccacggga tcgtgatgat gattcgtctg
    20341 ccggatttca atatgttgag cgatacttgg gggcacagcc aggttgaaga acagttcttc
    20401 actctgacga atctgctgtc gacatttatg atgcgctacc ctggcgcact gctggcgcgt
    20461 taccaccgca gtgattttgc tgcgctgtta ccgcaccgga cgttaaaaga ggcagagagc
    20521 atcgccggtc agttaatcaa agccgtcgat accttgccga acaataaaat gctcgatcgc
    20581 gacgatatga tccacattgg tatctgcgcc tggcgtagtg gtcaggatac cgagcaggta
    20641 atggaacatg cagagtctgc cacgcgtaat gcgggattgc agggcggcaa tagctgggct
    20701 atttacgatg actcgttgcc tgaaaaagga cgcggtaatg ttcgctggcg tacgcttatc
    20761 gagcaaatgc tcagtcgcgg cggcccgcgc ctttatcaaa aaccggcggt tactcgcgaa
    20821 ggtcaggttc atcatcgcga actcatgtgc cgcatcttcg atggtaatga agaggttagc
    20881 tcggcggagt atatgccgat ggtcttgcag tttggcttat cggaagagta tgaccgtctg
    20941 caaatcagcc gtcttattcc actattgcgt tactggccag aggaaaatct ggcgattcag
    21001 gttaccgttg agtcgctgat tcgcccgcgt tttcagcgtt ggctgcgcga tacgttaatg
    21061 caatgtgaaa aatcacaacg aaaacgcata attattgaac ttgcagaggc cgatgtaggt
    21121 caacatatca gtcgtttaca acctgttatt cgtttagtga atgctttagg ggtacgggta
    21181 gccgtcaacc aggctggttt gacgctggta agcaccagtt ggatcaaaga acttaatgtt
    21241 gagttactca agctccatcc ggggctggtc agaaacattg agaagcgaac ggagaaccag
    21301 ctgctggttc aaagcctggt ggaagcctgc tccgggacca gcacccaagt ttacgccacc
    21361 ggtgtgcgtt cgcgaagcga gtggcagacc ctgattcagc gcggtgttac aggcgggcaa
    21421 ggggattttt tcgcgtcctc acagccactt gatactaacg tgaaaaaata ttcacaaaga
    21481 tactcggttt aacctgccgt ttaatctgtt ttcacgtaga ataacgcgcg ctgcgtctca
    21541 tgggagtgtg cttgtctgct cgccagattg ttgcagcaca tatgcagatg aatgacctta
    21601 cgcggttgca aacaggcgag gaatgctgct gatgcattaa gcctttctgg actcaggcag
    21661 agatttgtaa caaaggaaac gaactgcact aattttcacc gtagcagatg atttttgcgc
    21721 cttgtcgctg ctgcgtgtgg ttggtaaagt aagcggattt tcttttccgc cccagctttc
    21781 aggattatcc cttagtatgt tgaaaaaatt tcgtggcatg ttttccaatg acttgtccat
    21841 tgacctgggt actgcgaata ccctcattta tgtaaaagga caaggcatcg tattgaatga
    21901 gccttccgtg gtggccattc gtcaggatcg tgccggttca ccgaaaagcg tagctgcagt
    21961 aggtcatgac gcgaagcaga tgctgggccg tacgccgggc aatattgctg ccattcgccc
    22021 aatgaaagac ggcgttatcg ccgacttctt cgtgactgaa aaaatgctcc agcacttcat
    22081 caaacaagtg cacagcaaca gctttatgcg tccaagcccg cgcgttctgg tttgtgtacc
    22141 ggttggcgcg acccaggttg aacgccgcgc aattcgtgaa tccgcgcagg gcgctggtgc
    22201 ccgtgaagtc ttcctgattg aagaaccgat ggctgccgca attggtgctg gcctgccggt
    22261 ttctgaagcg accggttcta tggtggttga tatcggtggt ggtaccactg aagttgctgt
    22321 tatctccttg aacggcgtgg tttactcctc ttccgtgcgc attggtggtg atcgtttcga
    22381 cgaagctatc atcaactatg tgcgtcgtaa ttacggttct ctgatcggtg aagccaccgc
    22441 agaacgtatc aagcacgaaa tcggttcggc ttacccgggc gatgaagtcc gtgaaatcga
    22501 agttcgtggc cgtaacctgg cagaaggtgt tccacgcggt tttaccctga actccaacga
    22561 aatcctcgaa gcactgcagg aaccgctgac cggtattgtg agcgcggtaa tggttgcact
    22621 ggaacagtgc ccgccggaac tggcttccga catctccgag cgcggcatgg tgctcaccgg
    22681 tggtggcgca ctgctgcgta accttgaccg tttgttaatg gaagaaaccg gcattccagt
    22741 cgttgttgct gaagacccgc tgacctgtgt ggcgcgcggt ggcggcaaag cgctggaaat
    22801 gatcgacatg cacggcggcg acctgttcag cgaagagtaa tcggatgcag gcaggggaaa
    22861 tgtctgttta ccctgcctgg tctgatacga gaatacgcat aacttatgaa gccaattttt
    22921 agccgtggcc cgtcgctaca gattcgcctt attctggcgg tgctggtggc gctcggcatt
    22981 attattgccg acagccgcct ggggacgttc agtcaaatcc gtacttatat ggataccgcc
    23041 gtcagtcctt tctactttgt ttccaatgct cctcgtgaat tgctggatgg cgtatcgcag
    23101 acgctggcct cgcgtgacca actagaactt gaaaaccggg cgttacgtca ggaactgttg
    23161 ctgaaaaaca gtgaactgct gatgcttgga caatacaaac aggagaacgc gcgtctgcgc
    23221 gagctgctgg gttccccgct gcgtcaggat gagcagaaaa tggtgactca ggttatctcc
    23281 acggttaacg atccttatag cgatcaagtt gttatcgata aaggtagcgt taatggcgtt
    23341 tatgaaggcc agccggtcat cagcgacaaa ggtgttgttg gtcaggtggt ggccgtcgct
    23401 aaactgacca gtcgcgtgct gctgatttgt gatgcgaccc acgcgctgcc aatccaggtg
    23461 ctgcgcaacg atatccgcgt aattgcagcc ggtaacggtt gtacggatga tttgcagctt
    23521 gagcatctgc cagcgaatac ggatattcgt gttggtgatg tgctggtgac ctccggtctg
    23581 ggcggtcgtt tcccggaagg ctatccggtc gcggttgtct cttccgtaaa actcgatacc
    23641 cagcgcgctt atactgtgat tcaggcgcgt ccgactgcag gactgcaacg tttgcgttat
    23701 ctgctgctgc tgtggggcgc ggatcgtaac ggcgctaacc cgatgacgcc ggaagaggtg
    23761 catcgtgttg ctaatgaacg tctgatgcag atgatgccgc aggtattgcc ttcgccagac
    23821 gcgatggggc caaagttacc tgaaccggca acggggatcg ctcagccgac tccgcagcaa
    23881 ccgacgacag gaaatgcagc tactgcgcct gctgcgccca cacagcctgc tgctaatcgc
    23941 tctccacaaa gggctacgcc gccgcaaagt ggtgctcaac cgcctgcgcg tgcgccggga
    24001 gggcaatagt ggcgagctat cgtagccagg gacgttgggt aatctggctc tctttcctca
    24061 ttgcgctgtt gctgcaaatc atgccctggc cggataacct gattgttttc cggccaaact
    24121 gggtgttact catcttgttg tattggatcc tggccttgcc tcatcgcgta aatgtgggca
    24181 caggttttgt gatgggtgcc atactggatc tgatcagcgg ctcgacgctt ggcgtacgcg
    24241 tattggcgat gagcatcatt gcttacctgg tggcgctgaa ataccagctt ttccgcaacc
    24301 tcgcattatg gcagcaggcg ctggtcgtca tgttgctttc gctggtggtg gatattattg
    24361 ttttctgggc agagttttta gtgattaacg tctctttcag accggaagtg ttctggagta
    24421 gtgtagtcaa tggggtgctc tggccgtgga ttttcttgct gatgcgtaaa gtccgtcagc
    24481 agtttgcagt gcaataaagg tttctatgac ttctctgtat ttagcttccg gttctccgcg
    24541 tcgtcaggag ttacttgcgc aacttggcgt gacctttgaa cgtattgtta cgggcattga
    24601 ggagcagcgt cagccgcagg agagcgcgca gcagtatgtt gtgcgtctgg cgcgcgagaa
    24661 agcacaggcg ggtgtcgcgc aaacggcgca ggatctcccg gtgctgggtg cggatactat
    24721 cgttatcctg aatggagaag tgctggagaa accgcgcgac gcagagcatg cggcgcagat
    24781 gttgcgcaaa ttatcgggtc agacccatca ggtgatgacg gcagtggcgt tggccgacag
    24841 ccagcacatt ctcgattgcc tggtggtcac cgatgtgact ttcagaacgt taacagacga
    24901 agacatcgcg ggctatgtcg ccagcggtga accgttagat aaagcaggtg catacggtat
    24961 tcaggggctg ggtggctgtt ttgtcaggaa gataaatggc agctatcacg ccgtagtcgg
    25021 cttaccgctg gttgaaacgt atgaattatt aagtaatttt aacgcactgc gtgagaaaag
    25081 ggataaacat gacggctgaa ttgttagtaa acgtaacgcc ttcggaaacg cgagtggcgt
    25141 atattgatgg cggtattctg caggaaattc atattgaacg tgaggcgcga cgcggaatag
    25201 taggcaatat ctacaagggt cgtgtaagtc gtgtacttcc gggtatgcag gcggcttttg
    25261 tagatattgg gctggataaa gccgcgtttc ttcatgcatc cgatatcatg ccgcacaccg
    25321 aatgtgtggc gggtgaagaa caaaagcaat tcacggtgcg cgacatctcg gaactggttc
    25381 gtcaggggca agatctgatg gtgcaggtgg tgaaagatcc gcttggcact aaaggtgcgc
    25441 gcctgaccac cgatatcacg ctgccttctc gctatctggt gtttatgcca ggggcttctc
    25501 acgttggggt ttcccaacgt attgaaagcg aatcagaacg tgaacgcctg aaaaaagtgg
    25561 tcgcagagta ttgcgacgag cagggcgggt ttatcatccg taccgcagcg gaaggggttg
    25621 gcgaggctga actggcctcc gatgccgctt atctgaaacg cgtctggacc aaagttatgg
    25681 agcgtaaaaa acgcccgcag acccgttatc agctgtacgg cgaactggcg ctggcgcagc
    25741 gtgttctgcg tgatttcgcc gacgccgaac tggaccgcat tcgcgttgac tcacgcctga
    25801 cttacgaagc attgctggag tttacctcgg agtacattcc cgagatgacc agcaagctgg
    25861 agcattacac cggacgccag ccgattttcg atctctttga tgtcgaaaac gaaatccagc
    25921 gagcgctgga acgcaaagta gaactgaaat ccggtggtta tctgattatc gaccagaccg
    25981 aagcgatgac caccgtggac atcaataccg gagcgtttgt cggtcatcgc aatctggacg
    26041 acaccatttt caataccaat attgaagcga cgcaggctat cgctcgccag ttacggttgc
    26101 gtaatctggg tgggattatc attattgatt tcatcgatat gaataatgaa gatcaccgcc
    26161 gccgcgtgct gcactcgctg gagcaggcgt tgagcaaaga ccgggtgaaa accagcgtta
    26221 atggtttttc ggcgctgggg ctggtggaga tgacgcgtaa acgcacccgc gaaagcattg
    26281 agcacgtact gtgtaacgaa tgcccaacct gccacggtcg cggaacggtg aaaaccgtgg
    26341 aaacggtatg ctatgaaatc atgcgcgaga ttgttcgtgt ccaccatgct tacgactccg
    26401 accgtttcct ggtctatgca tctccggcag tagctgaagc cttgaaaggc gaagagtcac
    26461 actcgctggc ggaagtggaa attttcgttg gcaaacaggt taaagtacaa attgaaccgc
    26521 tctataacca ggagcagttt gacgtcgtaa tgatgtaaac agatgctggg ccgccatccg
    26581 gcaaagggtt tttgagtcac atttttagca gacaaggagt gacgggtgag gcgattgccg
    26641 gggattttac tgcttactgg agccgcgctc gttgtgatcg ctgctctgct ggttagcggc
    26701 ctgcgtattg ctttaccgca tcttgacgcc tggcgtccgg aaatcctcaa caaaatagaa
    26761 tccgcgactg gcatgccggt agaagccagt cagctctcag ccagctggca gaattttggc
    26821 ccgacgcttg aagcacacga catccgtgca gaactaaaag atggcggcga attttcggtt
    26881 aaacgcgtta ctctggcgct ggatgtctgg cagagcctgt tacatatgcg ctggcagttt
    26941 cgcgacctca ctttctggca gctgcgcttt cgcaccaaca ctcctatcac cagcggtggt
    27001 agcgatgaca gtctggaagc cagtcacatc agcgatctgt ttcttcgtca atttgaccat
    27061 ttcgatctcc gcgacagtga agtcagtttt ctgacgccat ccggtcagcg cgccgagctg
    27121 gcgatcccac aactcacctg gctgaacgat ccacgtcgac accgtgcgga aggcctggta
    27181 agcctctcca gccttaccgg acagcacggc gtgatgcagg tgcgcatgga tttgcgcgat
    27241 gatgaggggt tgttaagcaa tggtcgcgtc tggctacagg cggatgacat cgacctgaag
    27301 ccgtggctcg gtaaatggat gcaggacaat attgctctgg aaacggcaca gttctccctt
    27361 gaaggctgga tgacgatcga caaaggcgat gtaaccggcg gtgacgtctg gctgaaacag
    27421 ggcggtgcca gctggttggg cgagaagcaa acgcatacgc tgtcggtgga taatctgacc
    27481 gcgcatatta cgcgtgaaaa tccgggctgg cagttctcta ttcccgatac acggatcacg
    27541 atggacggca aaccctggcc gagcggagca ttgacgctgg cctggatacc ggaacaggac
    27601 gttggcggca aagacaataa acgcagtgac gaactccgga ttcgcgccag taatctggag
    27661 ctggcaggcc tggagggcat acgcccgctg gccgcgaaac tttcacctgc actgggtgat
    27721 gtttggcgct ccacacaacc gagcggcaag attaacactc tggcgctgga tatcccgctt
    27781 caggcggcag acaagacccg ttttcaggca tcgtggagcg atctggcctg gaagcaatgg
    27841 aaattattac cgggtgcgga acacttctcc gggacgcttt ccggcagcgt tgaaaatggt
    27901 ttgcttaccg cgtcgatgaa gcaggcaaag atgccttacg aaacggtatt ccgtgcgcca
    27961 ctggaaatcg ccgacggcca ggcaactata agctggctga acaatgacaa aggtttccag
    28021 ctggatgggc gtaatattga cgttaaagcc aaagccgtcc atgcgcgcgg cggttttcgt
    28081 tacctgcaac ctgctaacga tgaaccctgg ctgggtattc tggctggcat cagtaccgat
    28141 gatggttcac aagcctggcg ctatttcccg gaaaacttga tgggtaaaga cctggttgat
    28201 tacttaagtg gcgcgattca gggcggtgaa gcggataacg cgacgctggt ttatggtggc
    28261 aatccgcaac tcttccccta taaacacaac gaaggtcagt ttgaagtgct ggtgccgctg
    28321 cgcaacgcga agtttgcctt ccagccggac tggcctgcat taactaacct tgatattgaa
    28381 ctggacttta ttaacgacgg tttatggatg aaaaccgatg gcgttaatct gggcggcgtg
    28441 cgcgcgagta atctcaccgc agtgatccct gactactcga aagaaaaact gctgattgac
    28501 gctgacatta aaggtccggg taaagccgtt ggcccttact ttgatgagac accgctgaaa
    28561 gattctctgg gtgcgaccct gcaagaactc cagctcgacg gcgatgtgaa tgctcgctta
    28621 catcttgata tcccgctgaa cggcgaactg gtaaccgcga aaggtgaagt gacgctgcgt
    28681 aataacagtc tgtttatcaa accactcgac agcaccctga aaaatttgag cggtaaattc
    28741 agctttatca atggcgatct gcaaagtgaa ccactgacag caagctggtt taatcagccg
    28801 ttgaacgtgg atttttccac caaagaaggg gcaaaagcct accaggttgc ggtgaatctc
    28861 aacggtaact ggcaaccggc gaaaaccagc gttctgcctg aagcggtgaa cgaagcattg
    28921 agtggcagcg tggcgtggga tggtaaagtg ggcattgatc tgccttatca tgctggcgcg
    28981 acctataacg tagagctgaa cggcgatctg aataatgtga gtagtcactt accttcaccg
    29041 ttagccaaac ctgcgggtga accactagcg gtaaacgtta aggttgatgg caatctcaac
    29101 agctttgaat taaccggaca ggctggtgcg gataaccatt tcaatagccg ctggttgctt
    29161 ggtcaaaagc tgacgctcga tcgtgctatt tgggcggcag acagtaaaac gctcccgccg
    29221 ttgccggaac aaagtggcgt tgaactcaat atgccgccga tgaatggtgc cgagtggctg
    29281 gccctgttcc agaaaggcgc tgcggagagt gtcggtggtg cagcgagttt cccacaacac
    29341 ataacgttac gtacgcctat gttgtcactg ggaaatcagc aatggaataa cctgagtatt
    29401 gtttcgcaac cgacggcaaa tggcaccctg gttgaggcgc aagggcgtga aatcaacgcc
    29461 acgctagcga tgcgtaataa cgcgccgtgg ctggcgaata tcaaatatct ttattacaac
    29521 ccgagcgtgg cgaaaactcg tggtgattca acgccgtcat cacctttccc gacaacggag
    29581 cgcattaact tccgtggctg gccggacgca caaatacgat gcacagagtg ctggttctgg
    29641 gggcaaaaat tcggtcgcat tgacagtgat atcaccattt ctggcgatac gttaacgctg
    29701 accaatggac tgattgatac tggtttctcg cggcttactg ccgatggtga atgggttaat
    29761 aatccggaga atgaacgtac ctcgctgaaa ggaaaactgc gcgggcagaa aattgatgcc
    29821 gccgcagaat tttttggtgt cacgacgccc atacgccagt cgtcatttaa tgtggattac
    29881 gatttacact ggcgcaaagc accctggcaa ccagatgagg cgacgttgaa tggcatcatt
    29941 catactcaac tgggtaaagg cgaaattacc gaaatcaata ccggacatgc cgggcaattg
    30001 ctgcgcttat tgagcgttga tgccctgatg cgtaagctgc gttttgattt cagagacact
    30061 tttggcgaag ggttctattt tgactccatt cgcagcaccg cgtggattaa agacggggtt
    30121 atgcacaccg acgacacgct ggtggatggc ctggaggcgg atatcgccat gaaagggtcg
    30181 gtaaatctgg tacgtcgcga cctgaatatg gaagcggttg tcgcaccaga gatttctgcg
    30241 acggtgggcg tggctgcggc ttttgcggtt aaccccattg ttggcgcggc agtatttgca
    30301 gccagtaaag tgctggggcc gctgtggagc aaagtctcca ttttgcgcta tcacatttcg
    30361 ggtccgctgg acgatccgca aatcaacgaa gtgttgcgcc aaccgcgtaa agaaaaagcg
    30421 caatgatttg acgagggcgc gtaattgccc caatctcata tgataatcgt tgccaaaggc
    30481 caacgagccg gaacataact gtaggccgga tagggcgttc acgccgcatc cggcagccgt
    30541 aaaaaatcct ctactgcagt aactaacgag tagcaaaaac gatgagtctg aacctggtaa
    30601 gtgaacaatt gctagcggcg aacggcctga aacatcagga cttgttcgcg atcctcggtc
    30661 aactggccga acgtcgcctt gattatggcg atctctattt tcagtcgagc tatcacgaat
    30721 cctgggtttt agaagaccgc attattaaag atggttctta caacatcgat cagggcgttg
    30781 gtgtgcgtgc aatcagtggt gaaaaaaccg gatttgctta cgctgaccaa atcagcctgc
    30841 tggcgctgga acagagtgcg caagcggcgc gcaccatcgt ccgtgatagt ggtgatggca
    30901 aagtacagac gctgggcgcg gtagagcata gcccgttgta tacctcggta gatccgctgc
    30961 aaagcatgag ccgtgaagag aagctggata tcctgcgtcg cgtcgataag gttgcccgcg
    31021 aagcggacaa gcgcgtacaa gaagtgactg ccagcctcag cggcgtttat gaattaattc
    31081 tggttgcggc caccgacggc acgctggcgg cggatgttcg tccgctggtg cgtctttccg
    31141 tgagcgttct ggtcgaagaa gatggcaaac gcgaacgcgg tgccagtggc ggcggcggtc
    31201 gttttggtta tgagttcttc cttgccgatc ttgacggcga agtccgtgcg gatgcatggg
    31261 caaaagaagc agtgcgtatg gcgctggtca atctttctgc cgttgctgca ccagcgggca
    31321 ccatgccggt agtacttggc gcaggttggc cgggcgtgct gttgcatgaa gcggtaggtc
    31381 acggtctgga aggcgacttc aactgccgcg gcacttcagt atttagtggg caggtcgggg
    31441 agctggtggc ttcagaactg tgtaccgtgg ttgatgacgg cacgatggtc gatcgccgtg
    31501 gttcggtggc gattgatgac gaaggtacgc caggccagta caacgtgctg attgagaacg
    31561 gcattctgaa aggctacatg caggataaac tcaacgcgcg tttgatgggg atgacgccga
    31621 ctggcaacgg tcgccgtgaa tcctacgccc atctgcccat gccgcgtatg accaacacct
    31681 atatgctgcc gggtaaatcg accccgcagg aaattattga atccgttgag tacggtatct
    31741 atgcaccgaa ctttggtggc ggtcaggtgg atattacatc cggcaaattc gttttctcca
    31801 cttcagaagc gtatctgatt gaaaacggta aagtaacgaa gccggtgaaa ggcgcgacgt
    31861 tgattggttc cggtatcgaa accatgcagc agatttcgat ggttggcaac gacctgaaac
    31921 tggataacgg cgtgggtgtc tgcggtaaag aagggcaaag tttgccggtt ggcgtgggcc
    31981 agccaacgct gaaagttgat aacctgactg ttggcggtac tgcgtaataa tttcattatt
    32041 ttcagaagga taattaatct ttctacgtgc acgaacggtc ccctcgctcc tctggggtta
    32101 gggtgagggg aacccgttgg cacaggtttg tagaacgtaa cagtacaata tgaattactt
    32161 ctctttcccg cgcccgtgca tctcctgaaa caatttaccg acctcaacaa aataatccgt
    32221 cagcgagttg atcacgacct gtaccttcag cggcagctta tctttttcgg tatataacgc
    32281 ataaaccggg cgtggatctg actgataacg cggcagcagg atctccagct caccgcgatt
    32341 gatctcgttg atcacccaca tcagcggcac gtaggcgatc ccggcacccg ccgttagcca
    32401 gcgcaccagc gtcatcggat cattagtcac aaatctccct tgcgggatca ggcgagtcga
    32461 gatcccttcc ggtgcaatca gttcaaattc attgtcgggc cgcacgctgt attcaagcca
    32521 tgaatggcta cttaaatcgg cgggtttttc cggtatgccg tattgtgtga gatagctttt
    32581 cgcggcgcac accaccattg gcatcgcgcc aagacggcgg gaaaacaggc tggaatcctg
    32641 caacgcgccg acgcggatca ccacatccag accgtcggca atcaggtcgg gggctggaat
    32701 tccggtaacc agattgacgc tcaaacctgg gtattctttc agcattttgg ctgtcagccc
    32761 ggcgagaaca ttttgtgcca tagttgaaga acagccaatg cgtagcgtcc cgatgggggt
    32821 gttattgaag gcatacagtt gctcatgaac atcctgcact tcatgaagca tacgacggca
    32881 gccctggtag taaattctac cggcttcggt caggccaatg ctgcgtgtgc tacggtttaa
    32941 cagctttacc tgcaactcat cttccagttt tgacaccgtc tgactgatgg acgaaacgct
    33001 catctgtagc tgtctggcgg cggcggtaaa agagccaaat tcaactactt tggcaaacac
    33061 cgacatgcgt tttagtcgtt ccattattca ctctgactta aaagtgattt agatcacata
    33121 atatagataa cagcataaca gttacgctaa tatattaaat atcaatctac agcaatgttg
    33181 ctctcgcccg gctttccatg cccttctctg cggcgacaga tgctgaaaat aataacgcct
    33241 gctctctcta tacaaccaag gtcaacatga gtctgtttcc cgttatcgtg gtgtttgggc
    33301 tgtccttccc accgatattt tttgaattgc ttttatcact ggcgattttc tggctggtgc
    33361 gccgggtact tgtgccaaca ggtatctacg actttgtctg gcatccggcg ttgttcaaca
    33421 ccgcgctcta ttgctgcttg ttttatttga tatcgcgact gttcgtttga ggttgaagtg
    33481 aaaacactaa taagaaaatt ctcccgtacg gccatcacgg tcgtattagt cattctggcc
    33541 ttcatcgcaa tttttaatgc ctgggtctat tacaccgaat ccccctggac gcgtgacgcg
    33601 cgctttagcg ctgacgtcgt tgcgatcgcg ccggacgttt ctggactcat tacccaggtg
    33661 aatgttcatg ataaccagct ggtgaaaaaa ggacagatac tgttcaccat cgaccagccg
    33721 cgctatcaaa aggcgcttga ggaagcgcaa gccgatgttg cctattatca ggtactggca
    33781 caggagaaac gccaggaggc cggacgtcgt aaccgtctcg gtgtgcaggc gatgtctcgc
    33841 gaagagatcg accaggctaa caacgtacta caaacggttc tgcatcagtt agcgaaagcg
    33901 caggcgaccc gcgatctggc aaaactggat cttgaacgca cggtgatccg cgcgccagca
    33961 gatggctggg tgaccaacct caacgtctat accggtgagt ttattactcg aggatcaacg
    34021 gcggttgcgc tggtgaaaca gaactccttc tatgtactgg cctatatgga agaaactaag
    34081 ctggaagggg tgcgtccggg gtatcgtgca gagatcacgc cgcttggcag taacaaagtg
    34141 ctgaaaggga ctgttgatag tgttgccgct ggggtcacca acgccagcag cacgcgtgac
    34201 gacaaaggga tggcgaccat cgactctaac cttgaatggg tgcgtttggc gcaacgtgtt
    34261 ccggttcgta ttcgtctcga caaccagcaa gagaacatct ggcctgcggg caccactgct
    34321 acagtggtgg tcactggcaa acaagatcgc gacgaaagcc aggattcgtt cttccgtaaa
    34381 atggcccatc gcctgcgtga gtttggttaa tcacgatggg tattttctcc attgctaacc
    34441 aacatattcg ctttgcggta aaactggcga ccgccattgt actggcgctg tttgttggct
    34501 ttcacttcca gctggaaacg ccacgctggg cggtactgac agcggcgatt gttgccgccg
    34561 gtccggcctt tgctgcggga ggtgaaccgt attctggcgc tattcgctat cgtggctttt
    34621 tgcgcatcat cggcacattt attggctgta ttgccggact ggtgatcatc attgcgatga
    34681 tccgcgcacc attattgatg attctggtgt gctgtatctg ggccggtttt tgtacctgga
    34741 tatcctcgct ggtacgaata gaaaactcgt acgcgtgggg gctggccggt tataccgcgc
    34801 tgatcattgt gatcaccatt cagccggaac cattgcttac gccgcagttt gccgtcgaac
    34861 gttgtagcga gatcgttatc ggtattgtgt gtgcgattat ggcggatttg ctcttttctc
    34921 cgcgatcgat caaacaagaa gtggatcgag agctggaaag tttgctggtc gcgcaatatc
    34981 aattaatgca actctgtatc aagcatggcg atggtgaagt tgtcgataaa gcctggggcg
    35041 acctggtgcg acgcaccacg gcgctacaaa gcatgcgcag caacctgaat atggaatctt
    35101 cccgctgggc gcgggccaat cgacgtttaa aagcgatcaa tacgctatcg ctgacgctga
    35161 ttacccaatc ctgcgaaact tatcttattc agaatacgcg cccggaattg atcactgata
    35221 ctttccgcga attttttgac acgccggtag aaaccgcgca ggacgtccac aagcagctca
    35281 aacgcctgcg gagagttatc gcctggaccg gggaacggga aacgcctgtc accatttata
    35341 gctgggtcgc ggcggcaacg cgttatcagc ttctcaagcg cggcgttatc agtaacacaa
    35401 aaatcaacgc caccgaagaa gagatcctgc aaggcgaacc ggaagtcaaa gtagagtcag
    35461 ccgaacgtca tcatgcgatg gttaacttct ggcgaaccac actttcctgc attctgggca
    35521 cgcttttctg gctgtggacg ggctggactt ccggcagtgg tgcaatggtg atgattgcgg
    35581 tcgtgacgtc actggcaatg cgtttgccga atccacgcat ggtggcgatc gactttatct
    35641 acgggacgct ggccgcgctg ccgttagggc tgctctactt tttggtgatt atccctaata
    35701 cccaacagag catgttgctg ctgtgtatta gcctggcagt gctgggattt ttcctcggta
    35761 tagaagtaca gaaacggcga ctgggctcga tgggggcgct ggccagcacc ataaatatta
    35821 tcgtgctgga taacccgatg actttccatt tcagtcagtt tctcgacagc gcattagggc
    35881 aaatcgtcgg ctgtgtgctc gcgttcaccg ttattttgct tgtgcgggat aaatcgcgcg
    35941 acaggaccgg acgtgtactg cttaatcagt ttgtttctgc cgctgtttcc gcgatgacta
    36001 ccaatgtggc acgtcgtaaa gagaaccacc tcccggcact ttatcagcag ctgtttttgc
    36061 tgatgaataa gttcccaggg gatttgccga aatttcgcct ggcgctgacg atgattatcg
    36121 cgcaccagcg cctgcgtgat gcgccgatcc cggttaacga ggatttatcg gcgtttcacc
    36181 gacaaatgcg ccgcacagca gaccatgtga tatctgcccg tagcgatgat aaacgtcgtc
    36241 ggtactttgg ccagttgctg gaagaactgg aaatctacca ggaaaagcta cgcatctggc
    36301 aagcgccacc gcaggtgacg gaaccggttc atcggctggc ggggatgctc cataagtatc
    36361 aacatgcgtt gaccgatagt taagtcaaaa ccgacgccaa aagcgtcggt tttttcatgg
    36421 ctatacttag cgatgcacgg cagaactcgc cgcgaaacgt gacggtggca acagatgaat
    36481 atttatacct ttgattttga tgagattgag agtcaggagg atttttatcg tgactttagc
    36541 caaacctttg gtctggctaa agataaggta cgcgatctcg actcactatg ggatgtgtta
    36601 atgaacgatg tcctgccgct accacttgag attgaatttg ttcatctggg agagaaaacg
    36661 cgtcgccgtt ttggcgcgtt aatattgctg tttgatgagg cagaggaaga gctggaaggg
    36721 catttgcgtt ttaatgttcg tcattagcgc aaaaaaaagc ccccgaaccg ggggcaatat
    36781 cgtcggacaa gacgatgagg gtttatttgt acagttcagc cgtagcgtgc caggtgtcac
    36841 cgctacgagc ttcagtaatc tggtaggccg ttgcgccttt ctcttccgct tttttgttca
    36901 gcatttcacg catatccatt ggcgaagacg ccacaccact tacggatacg gtcccgattg
    36961 cttcacgatt ttgtgcttgt gcagcatcaa tggagtcggc agcgaatgca ccgaaagaga
    37021 gaacagaaag tacgcttaat gcagctacag tggttttgat tttcatgatt tttacctcga
    37081 cataatcttt tagctgggcc tttgtttcgt gacccatatc acaaaatca
//
LOCUS       DAZFWL020000036        36724 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFWL020000036 DAZFWL020000000
VERSION     DAZFWL020000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 36724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.036"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHKNP_RS09525"
                     /old_locus_tag="pgaptmp_001905"
     CDS             complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHKNP_RS09525"
                     /old_locus_tag="pgaptmp_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HGU4704079.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHKNP_RS09530"
                     /old_locus_tag="pgaptmp_001906"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHKNP_RS09530"
                     /old_locus_tag="pgaptmp_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4704080.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHKNP_RS09535"
                     /old_locus_tag="pgaptmp_001907"
     CDS             complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHKNP_RS09535"
                     /old_locus_tag="pgaptmp_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HGU4704081.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHKNP_RS09540"
                     /old_locus_tag="pgaptmp_001908"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHKNP_RS09540"
                     /old_locus_tag="pgaptmp_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HGU4704082.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="ACHKNP_RS09545"
                     /old_locus_tag="pgaptmp_001909"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="ACHKNP_RS09545"
                     /old_locus_tag="pgaptmp_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HGU4704083.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="ACHKNP_RS09550"
                     /old_locus_tag="pgaptmp_001910"
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="ACHKNP_RS09550"
                     /old_locus_tag="pgaptmp_001910"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGU4704084.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHKNP_RS09555"
                     /old_locus_tag="pgaptmp_001911"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHKNP_RS09555"
                     /old_locus_tag="pgaptmp_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HGU4704085.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7218..7283)
                     /locus_tag="ACHKNP_RS09560"
                     /old_locus_tag="pgaptmp_001912"
     CDS             complement(7218..7283)
                     /locus_tag="ACHKNP_RS09560"
                     /old_locus_tag="pgaptmp_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704086.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7518..8405
                     /gene="rhtA"
                     /locus_tag="ACHKNP_RS09565"
                     /old_locus_tag="pgaptmp_001913"
     CDS             7518..8405
                     /gene="rhtA"
                     /locus_tag="ACHKNP_RS09565"
                     /old_locus_tag="pgaptmp_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HGU4704087.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            8704..9207
                     /gene="dps"
                     /locus_tag="ACHKNP_RS09570"
                     /old_locus_tag="pgaptmp_001914"
                     /gene_synonym="pexB"
     CDS             8704..9207
                     /gene="dps"
                     /locus_tag="ACHKNP_RS09570"
                     /old_locus_tag="pgaptmp_001914"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HGU4704088.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            9611..10357
                     /gene="glnH"
                     /locus_tag="ACHKNP_RS09575"
                     /old_locus_tag="pgaptmp_001915"
     CDS             9611..10357
                     /gene="glnH"
                     /locus_tag="ACHKNP_RS09575"
                     /old_locus_tag="pgaptmp_001915"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HGU4704089.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            10496..11155
                     /gene="glnP"
                     /locus_tag="ACHKNP_RS09580"
                     /old_locus_tag="pgaptmp_001916"
     CDS             10496..11155
                     /gene="glnP"
                     /locus_tag="ACHKNP_RS09580"
                     /old_locus_tag="pgaptmp_001916"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HGU4704090.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVLYLIITLVLSFILRRLERRMKI
                     L"
     gene            11152..11874
                     /gene="glnQ"
                     /locus_tag="ACHKNP_RS09585"
                     /old_locus_tag="pgaptmp_001917"
     CDS             11152..11874
                     /gene="glnQ"
                     /locus_tag="ACHKNP_RS09585"
                     /old_locus_tag="pgaptmp_001917"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HGU4704091.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            11991..14216
                     /gene="ybiO"
                     /locus_tag="ACHKNP_RS09590"
                     /old_locus_tag="pgaptmp_001918"
     CDS             11991..14216
                     /gene="ybiO"
                     /locus_tag="ACHKNP_RS09590"
                     /old_locus_tag="pgaptmp_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HGU4704092.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPPPPGEPT
                     L"
     gene            complement(14213..15139)
                     /gene="rlmF"
                     /locus_tag="ACHKNP_RS09595"
                     /old_locus_tag="pgaptmp_001919"
     CDS             complement(14213..15139)
                     /gene="rlmF"
                     /locus_tag="ACHKNP_RS09595"
                     /old_locus_tag="pgaptmp_001919"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HGU4704093.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            15415..15660
                     /gene="mcbA"
                     /locus_tag="ACHKNP_RS09600"
                     /old_locus_tag="pgaptmp_001920"
                     /pseudo
     CDS             15415..15660
                     /gene="mcbA"
                     /locus_tag="ACHKNP_RS09600"
                     /old_locus_tag="pgaptmp_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
     gene            15938..18220
                     /gene="fiu"
                     /locus_tag="ACHKNP_RS09605"
                     /old_locus_tag="pgaptmp_001921"
     CDS             15938..18220
                     /gene="fiu"
                     /locus_tag="ACHKNP_RS09605"
                     /old_locus_tag="pgaptmp_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HGU4704094.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            18262..18939
                     /gene="ybiX"
                     /locus_tag="ACHKNP_RS09610"
                     /old_locus_tag="pgaptmp_001922"
     CDS             18262..18939
                     /gene="ybiX"
                     /locus_tag="ACHKNP_RS09610"
                     /old_locus_tag="pgaptmp_001922"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HGU4704095.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            19013..19279
                     /gene="ybiI"
                     /locus_tag="ACHKNP_RS09615"
                     /old_locus_tag="pgaptmp_001923"
     CDS             19013..19279
                     /gene="ybiI"
                     /locus_tag="ACHKNP_RS09615"
                     /old_locus_tag="pgaptmp_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HGU4704096.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKGLQKPAYTGYSRRGSKDSQLR"
     gene            19544..19804
                     /gene="ybiJ"
                     /locus_tag="ACHKNP_RS09620"
                     /old_locus_tag="pgaptmp_001924"
     CDS             19544..19804
                     /gene="ybiJ"
                     /locus_tag="ACHKNP_RS09620"
                     /old_locus_tag="pgaptmp_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HGU4704097.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(20036..20518)
                     /gene="ybiA"
                     /locus_tag="ACHKNP_RS09625"
                     /old_locus_tag="pgaptmp_001925"
     CDS             complement(20036..20518)
                     /gene="ybiA"
                     /locus_tag="ACHKNP_RS09625"
                     /old_locus_tag="pgaptmp_001925"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HGU4704098.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            20638..22788
                     /gene="dinG"
                     /locus_tag="ACHKNP_RS09630"
                     /old_locus_tag="pgaptmp_001926"
     CDS             20638..22788
                     /gene="dinG"
                     /locus_tag="ACHKNP_RS09630"
                     /old_locus_tag="pgaptmp_001926"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HGU4704099.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            22816..23778
                     /gene="ybiB"
                     /locus_tag="ACHKNP_RS09635"
                     /old_locus_tag="pgaptmp_001927"
     CDS             22816..23778
                     /gene="ybiB"
                     /locus_tag="ACHKNP_RS09635"
                     /old_locus_tag="pgaptmp_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HGU4704100.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNTAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            23919..25004
                     /gene="hcxB"
                     /locus_tag="ACHKNP_RS09640"
                     /old_locus_tag="pgaptmp_001928"
     CDS             23919..25004
                     /gene="hcxB"
                     /locus_tag="ACHKNP_RS09640"
                     /old_locus_tag="pgaptmp_001928"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HGU4704101.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHKHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGALLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            25071..25130
                     /gene="ybiE"
                     /locus_tag="ACHKNP_RS09645"
                     /old_locus_tag="pgaptmp_001929"
     CDS             25071..25130
                     /gene="ybiE"
                     /locus_tag="ACHKNP_RS09645"
                     /old_locus_tag="pgaptmp_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HGU4704102.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(25235..26599)
                     /gene="rhlE"
                     /locus_tag="ACHKNP_RS09650"
                     /old_locus_tag="pgaptmp_001930"
     CDS             complement(25235..26599)
                     /gene="rhlE"
                     /locus_tag="ACHKNP_RS09650"
                     /old_locus_tag="pgaptmp_001930"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HGU4704103.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGDGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            26828..27499
                     /gene="cecR"
                     /locus_tag="ACHKNP_RS09655"
                     /old_locus_tag="pgaptmp_001931"
     CDS             26828..27499
                     /gene="cecR"
                     /locus_tag="ACHKNP_RS09655"
                     /old_locus_tag="pgaptmp_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HGU4704104.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            27502..28497
                     /gene="hlyD"
                     /locus_tag="ACHKNP_RS09660"
                     /old_locus_tag="pgaptmp_001932"
     CDS             27502..28497
                     /gene="hlyD"
                     /locus_tag="ACHKNP_RS09660"
                     /old_locus_tag="pgaptmp_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HGU4704105.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            28490..30226
                     /gene="ybhF"
                     /locus_tag="ACHKNP_RS09665"
                     /old_locus_tag="pgaptmp_001933"
     CDS             28490..30226
                     /gene="ybhF"
                     /locus_tag="ACHKNP_RS09665"
                     /old_locus_tag="pgaptmp_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU4704106.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            30219..31352
                     /gene="ybhS"
                     /locus_tag="ACHKNP_RS09670"
                     /old_locus_tag="pgaptmp_001934"
     CDS             30219..31352
                     /gene="ybhS"
                     /locus_tag="ACHKNP_RS09670"
                     /old_locus_tag="pgaptmp_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4704107.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            31363..32469
                     /gene="ybhR"
                     /locus_tag="ACHKNP_RS09675"
                     /old_locus_tag="pgaptmp_001935"
     CDS             31363..32469
                     /gene="ybhR"
                     /locus_tag="ACHKNP_RS09675"
                     /old_locus_tag="pgaptmp_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4704108.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(32431..32841)
                     /gene="ybhQ"
                     /locus_tag="ACHKNP_RS09680"
                     /old_locus_tag="pgaptmp_001936"
     CDS             complement(32431..32841)
                     /gene="ybhQ"
                     /locus_tag="ACHKNP_RS09680"
                     /old_locus_tag="pgaptmp_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HGU4704109.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            32974..33735
                     /gene="ybhP"
                     /locus_tag="ACHKNP_RS09685"
                     /old_locus_tag="pgaptmp_001937"
     CDS             32974..33735
                     /gene="ybhP"
                     /locus_tag="ACHKNP_RS09685"
                     /old_locus_tag="pgaptmp_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU4704110.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPKGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            33732..34973
                     /gene="clsB"
                     /locus_tag="ACHKNP_RS09690"
                     /old_locus_tag="pgaptmp_001938"
     CDS             33732..34973
                     /gene="clsB"
                     /locus_tag="ACHKNP_RS09690"
                     /old_locus_tag="pgaptmp_001938"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HGU4704111.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            34973..35929
                     /gene="ybhN"
                     /locus_tag="ACHKNP_RS09695"
                     /old_locus_tag="pgaptmp_001939"
     CDS             34973..35929
                     /gene="ybhN"
                     /locus_tag="ACHKNP_RS09695"
                     /old_locus_tag="pgaptmp_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HGU4704112.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPVGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(35965..36678)
                     /gene="ybhM"
                     /locus_tag="ACHKNP_RS09700"
                     /old_locus_tag="pgaptmp_001940"
     CDS             complement(35965..36678)
                     /gene="ybhM"
                     /locus_tag="ACHKNP_RS09700"
                     /old_locus_tag="pgaptmp_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU4704113.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
BASE COUNT         8828 a         9601 c         9503 g         8792 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca attcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtattta acttccagat cggccacttt atagccgtct
     1321 tcctcactca tttccggcaa agcatagatg cggtcgcgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa agtcggctct
     1501 aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc aaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg ggtctgtatg cactctacat cggtcgcctg tatgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt cccctcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtatcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaacttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacatgg tcggcatatt tatttttgtg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagagtgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggtg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa tcggcgaata
     5941 cgatactgct atcgccagca tcgccaaacg tgcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa acacgaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttctg catctgatgg gctcgcatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcaatcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctctctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aaatatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gctttttctc gcagtggacg ggaattaaag cgaaggaaat
     6481 aaatatcaaa tacccgttta tatctgagaa gaaagccggg ccgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaaacctta
     6601 aaacaaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtacctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattccgt ggtctggaat ttaccataac
     6841 ccacccccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agagccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
     7201 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct aacgggccat
     7261 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
     7321 agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac
     7381 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca
     7441 acaaatgctc aaccatcatt gggtatatcc agtacactcc acgctttact taagtctaga
     7501 tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaataa
     7561 tcatattgct cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt
     7621 ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga acgctgatcc
     7681 tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacgg ttaccgctgt
     7741 tgttttacgg cgtttcgctg ggtgggatga attatctttt ttatctttct attcagacag
     7801 taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg gcgctgttct
     7861 cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc
     7921 tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg
     7981 gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg
     8041 gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggag
     8101 cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg
     8161 ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa
     8221 cacggacatt tggtacgctg atgagcatgg aaccggcgct ggctgccgtt tccgggatga
     8281 ttttcctcgg agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg
     8341 cttcaatggg gtctacgctg acagtacgca aagagagcaa aataaaagaa ttagacatta
     8401 attaaattta catttctgca tggttatgca taaccatgca gaatttctcg ctacttttcc
     8461 tctacaccgt ctttatatat cgaattatgc aaaagcatat ttattccgaa aattcctggc
     8521 gagcagataa ataagaattg ttcttatcaa tatatctaac tcattgaatc tttattagtt
     8581 ttgtttttca cgcttgttac cactattagt gtgataggaa cagccagaat agcggaacac
     8641 atagccggtg ctatacttag tctcgttaat tactgggaca taacatcaag aggatatgaa
     8701 attatgagta ccgctaaatt agttaaatca aaagcgacca atctgcttta tacccgcaac
     8761 gatgtctccg acagcgagaa aaaagcaaca gtagagttgc tgaatcgcca ggttatccag
     8821 tttattgatc tttctttgat taccaaacaa gcgcactgga acatgcgcgg cgctaacttc
     8881 attgccgtac atgaaatgct ggatggcttc cgcaccgcac tgatcgatca tctggatacc
     8941 atggcagaac gtgcagtgca gctgggcggt gtagctctgg ggaccactca agttatcaac
     9001 agcaaaaccc cgctgaaaag ttacccgctg gacatccaca acgttcagga tcacctgaaa
     9061 gaactggctg accgttacgc aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg
     9121 aaagatgacg acaccgcaga tatcctgacc gccgcgtctc gcgacctgga taaattcctg
     9181 tggtttatcg agtctaacat cgaataaatc catcgctgat ggtgcagaac tttagtaccc
     9241 gataaaagcg gcttcctgac aggaggccgt tttgttttgc agcccacctc aacgcactta
     9301 tttagtgcat ccatctgcta tctccagctg attaagtaaa ttttttgtat ccacatcatc
     9361 acacaatcgt tacataaaga ttgttttttc atcaggtttt acgctaaata atcactgtgt
     9421 tgagtgcaca attttagcgc accagattgg tgccccagaa tggtgcatct tcagggtatt
     9481 gccctataaa tcgtgcatca cgtttttgcc gcatctcgaa aaatcaatga gttgcaaaac
     9541 tggcacgatt ttttcatata tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa
     9601 aggaaatgct atgaagtctg tattaaaagt ttcactggct gcactgaccc tggcttttgc
     9661 ggtttcttct catgccgcgg ataaaaaatt agttgtcgcg acggataccg ccttcgttcc
     9721 gtttgaattt aaacagggcg ataaatatgt gggctttgac gttgatctgt gggctgccat
     9781 cgctaaagag ctgaagctgg attacgaact gaagccgatg gatttcagtg ggatcattcc
     9841 ggcactgcaa accaaaaacg tcgatctggc gctggcgggc attaccatca ccgacgagcg
     9901 taaaaaagcg atcgatttct ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa
     9961 agctaacaat aacgatgtga aaagcgtgaa agatctcgac gggaaagtgg ttgctgtgaa
    10021 gagcggtact ggctccgttg attacgcgaa agcaaacatc aaaactaaag atctgcgtca
    10081 gttcccgaac atcgataacg cctatatgga actgggcacc aaccgcgcag acgccgttct
    10141 gcacgatacg ccaaacattc tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc
    10201 ggtaggtgac tctctggaag cgcagcaata cggtattgcg ttcccgaaag gtagcgacga
    10261 gctgcgtgac aaagtcaacg gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga
    10321 aatctacaaa aaatggttcg gtactgaacc gaaataataa cgctacacct gtaaaacgca
    10381 ctggcagttc cctctcccct ctggggagag gattagggtg aggggcgcaa acccgctccg
    10441 gggccattaa ttaccctgaa tttgattatt tacaccacgg taacaggaac aacatatgca
    10501 gtttgactgg agtgccatct ggcctgccat tccgcttctg attgaaggtg ccaaaatgac
    10561 cctgtggatt tcggtcctcg gtctggcagg cggtctggta atcggattgc tggcaggttt
    10621 tgcacgcacc ttcggtggtt ggatagccaa ccacgtcgcg ctggtcttta ttgaagtgat
    10681 ccgcggcaca cctatcgtcg tccaggtgat gtttatctat ttcgccctgc cgatggcgtt
    10741 taacgactta cgcatcgacc catttactgc ggcggtggtc accatcatga tcaactccgg
    10801 cgcgtacatt gcggaaatca cgcgtggtgc ggtgctgtct atccacaaag gttttcgtga
    10861 agcaggactg gcgctcggtc tttcacgttg ggaaaccatt cgctacgtca ttttaccgct
    10921 ggcactgcgt cgtatgctgc cgccgctggg taaccagtgg atcatcagca ttaaagacac
    10981 ctcgctgttt attgtgatcg gcgtggcgga actgacccgt caggggcaag aaattattgc
    11041 cggtaacttc cgcgcccttg agatctggag cgccgtggcg gtgctctatc tgattattac
    11101 cctggtgctg agctttattc tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa
    11161 tttaaaaacg tctccaagca ctttggccca acccaggtgc tgcataatat cgatttgaac
    11221 attgcccagg gcgaagtcgt ggtgattatc gggccgtccg gttccggtaa atcgaccctg
    11281 ctgcgctgca tcaacaaact ggaagaaatc acctccggcg atctgattgt cgatggcctg
    11341 aaggttaacg atccgaaagt tgacgagcgc ctgattcgcc aggaagcagg tatggtgttc
    11401 cagcagtttt acctcttccc gcatctgacg gcgctggaaa acgtcatgtt tggtccgcta
    11461 cgcgtgcgtg gcgcgaacaa agaagaggcg gaaaaactgg cgcgtgagct gctggcgaaa
    11521 gtcggtctgg cagaacgtgc acatcactac ccttccgaac tttctggtgg tcaacagcag
    11581 cgtgtggcga ttgcccgcgc gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg
    11641 acttccgctc ttgacccgga actgcgccat gaagtgctga aggttatgca ggatctggct
    11701 gaagaaggga tgacgatggt gatcgtgacc cacgaaatcg gttttgccga gaaagtagct
    11761 tcgcggctga tctttatcga caaaggccgg attgcggaag atggcgatcc gcaggtattg
    11821 atcaagaacc cgccgagcca gcgcttgcag gaatttttgc agcacgtctc ttaacttctc
    11881 tgcctccctt tcacccgaaa gggaggcaca ccagattcct ctcatttaaa atcgcccctc
    11941 ccccagcatc tatacttatc tttttgctct gttttctcac tggaggagtc atgcggtgga
    12001 tcctgttcat cctcttctgc ctgctgggcg cacctgccca cgcggtatcc atacccggcg
    12061 ttacaaccac aacgacaacg gactcaacga ctgaaccggc cccggaaccg gatatcgaac
    12121 aaaaaaaagc ggcctatggc gcactggcgg atgtgctgga taacgacacc tcgcgtaaag
    12181 aattgatcga ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg gtcccgaaga
    12241 tcgtgccgcc gacgctggtt gaagagcaaa ccgtgctgca aaaggtcacc gaagtcagcc
    12301 gccattatgg tgaagccctt tccgcccgct tcgggcaact ttatcgcaat atcaccggct
    12361 ccccgcataa gccatttaat ccacaaacct tcagcaatgc gctgagccat ttttcaatgt
    12421 tagcggtatt agtgtttggt ttttactggc tgattcgcct gtgcgcactg ccgctgtatc
    12481 gcaaaatggg tcagtgggcg aggcaaaaaa atcgtgagcg cagcaactgg ttgcagcttc
    12541 cggcgatgat tatcggggcg tttattatcg acctgctgtt actggccctg acattatttg
    12601 tcggccaggt attaagcgac aacctgaatg caggcagtcg caccatcgct ttccaacaaa
    12661 gtttgtttct caatgccttt gccctgattg aatttttcaa agccgtacta cgcctgattt
    12721 tttgcccaaa cgtggcggag ctgcgtccgt tcacgattca ggacgagagc gcccgttact
    12781 ggagtcgtcg cctgagctgg ttaagcagtt tgataggtta tggcctgatt gtggccgtgc
    12841 cgattatctc taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc atcattatgc
    12901 tgtgcatgac cgtctgggcg ttgtacctga tctttcgtaa taaaaaagag attacccagc
    12961 atttgctcaa ctttgcggag cattcgctgg cctttttcag cctgtttatc cgcgcctttg
    13021 cgctggtgtg gcactggctg gcaagcgcct attttatcgt gctgtttttc ttttcgttgt
    13081 tcgatccggg caacagcctg aaatttatga tgggtgcaac ggtgcgcagc ctggcgatta
    13141 ttggtatcgc agcgtttgtt tccggtatgt tttcccgctg gctggcgaaa accatcaccc
    13201 tctcgccaca tactcagcgt aactatccgg agctacaaaa acggctgaat ggctggcttt
    13261 cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt ggcagtaatg ttgctgttga
    13321 gcgcatgggg attgttcgat ttctggaact ggctgcaaaa cggcgcgggg cagaaaaccg
    13381 tagatatcct gatccgcatc gcactcattc ttttcttctc ggcggttggc tggacggtgc
    13441 tcgccagttt gatcgaaaac cggctggctt cggatattca tggccgcccg ctacccagcg
    13501 cccgtacgcg taccctgctg acgctgtttc gtaacgcgct ggcggtgatt atcagtacca
    13561 tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg ctggcaggtg
    13621 ccggggcatt aggtctggct atctcgtttg gttcgcaaac gctggtgaaa gatatcatta
    13681 ccggggtgtt tattcagttt gaaaacggca tgaacactgg agatttggtg actatcgggc
    13741 cgttgaccgg cacagtggaa cggatgtcga ttcgctccgt gggcgtgcgc caggataccg
    13801 gggcgtatca catcattccg tggtcttcga taaccacctt tgctaacttc gtccgcggta
    13861 ttggttcggt agtggcaaac tacgatgttg atcgccatga agatgctgat aaagccaatc
    13921 aggcactgaa agatgcggta gcggaattga tggaaaacga agaaattcgc gggctgatta
    13981 ttggtgaacc gaattttgcc gggattgtcg gcttaagcaa taccgcgttt acactgcgtg
    14041 tttcgttcac cacgctgcca ctcaaacagt ggacggtacg ctttgccctc gacagccagg
    14101 tgaaaaaaca tttcgacctg gcgggcgttc gcgcgccagt gcagacttat caggtgctgc
    14161 ctgctccggg cgcgaccccg gctgaaccgc cgccgccggg ggagccaacg ctttaacgtt
    14221 ggcgattaac aaaacggcgg cgctgctcgt cgttcataaa ggtccaggca ataaagcgac
    14281 tctgcttttg cccctgggcc atctcttttt ttaccacctt caccgcgccc acgtcggtca
    14341 gagcacgata caacggcggt aagttttcac cacgagatac cagcgaggta aaccacatca
    14401 cctgcttcgc gaagcctttg ctctcttcaa tcatcttttt gataaaggcg acttcaccac
    14461 cttcacacca caactcctgt tgctgaccac caaagttcag tgcatcgtct ttgttcagcc
    14521 ccaggttacg gcgtttacgc tcactacctg cccgcgctgc agcggcggaa tcgtggaatg
    14581 gcgggttaca caaggtcgcg tcgtattgct cgtttttatg gatgatgccg ttaaaaattg
    14641 ccccactctc tttttgccga cgcagacgaa tggcgcggtt aagccccgga ttagcactga
    14701 taatcgcctg cgcactgctt aacgcctggc tgctggtttc actaccggta aaacgccagc
    14761 catattcatg tacgccaatc agcggataaa tacagttcgc accaacgccg atatccagaa
    14821 tgctggcatt cgctggaatt gttccgcttg cttcagccag taaatcggca aggtgatgaa
    14881 tataatccgc ccggcccggt actggtgggc agagaaaacc gtcggggata tcccagttcg
    14941 ctacggcgta aaaatgggcc agcaacgcct tattgagcgc cttcaccgcc agcggattgg
    15001 caaagtctac gctttgctcc ccggcgggtg taagcgtgag gaattgcctg agttcaggat
    15061 tgacctgaca aagcgtggcg agatcgtagc ggctgtgatg acggttgcgc ggatgcaacc
    15121 ccggtttctg ggcggacata gcattctcct ttaagcagcg gcgtaagata cccgcataac
    15181 gcgacagggt aaatatctgg ctgcgtttat cattccccaa aaccttaaca tggctatggt
    15241 ttatggcaat tcatcataaa aatcagcatt atgcatgtca ttgatttgtc ttattttgta
    15301 aaaatatatc gttcaaaaaa taagcacatc tctgtcaatt ttttgcgtca ttttcatcgc
    15361 ccaggcgacc tacacttatc aggcactacc cgttgtattt atgaggatgt cattatgaaa
    15421 aagtgcctca cactactgat tgccaccgtc ctgagcggaa tctctctcac ggcttatgcc
    15481 gcgcaaccga tgagtaacct ggacagcggt cagctacggc ccgccggtac cgtttcggca
    15541 actggcgcat caaacctaag cgatctggag gataaactgg cagaaaaagc gcgcgagcaa
    15601 ggcgcgaaag gttatgtcat taattccgca ggcggaaatg accagatgtt cggtactgca
    15661 ccatctacaa ataaccccac caacgtaact gcattcgcct ctggatgcag ttttcgcctg
    15721 ttttgtaatc aaaaaacatc aaatatgaac gcaatgtaaa taaatgtatt tctttttcgc
    15781 gcaatgggtg atagaaaatc gcgccaagtg ataatgctta tcaaaattat tatcacttgc
    15841 acgaacacta tcacgagatt aacagtggca tcgcatccgc agagaggctt tctcgtggca
    15901 gtgaaaattt caacatataa aaaaagtcac ctgcaaaatg gaaaacaatc gcaatttccc
    15961 tgccagacaa tttcattcgc tcacgttctt tgccggtctt tgtattggca tcacgcctgt
    16021 ggctcaggca ctcgccgccg aagggcaaac taacgcggat gacacgctgg ttgtcgaagc
    16081 atcaacgcct tcgctttatg cgccacaaca atctgccgat ccgaaattct cgcgtccggt
    16141 agcggatact acccgcacga tgacggtgat ttctgaacaa gtgattaaag atcagggcgc
    16201 aaccaacctt accgacgcgc tcaaaaacgt ccccggcgtg ggtgcgtttt ttgcgggtga
    16261 gaacggtaac tccaccactg gcgacgccat ttatatgcgc ggtgctgata cctctaacag
    16321 tatttatatt gatggcattc gcgatatcgg cagcgtctcg cgcgacacct tcaatactga
    16381 gcaggtcgaa gtgattaaag ggccttccgg caccgactac gggcgcagcg cgccgacggg
    16441 ctcgatcaat atgatcagca agcagccgcg caatgattcc ggcattgacg cctccgccag
    16501 tattggcagc gcctggttcc gccgcggcac gctggacgtc aatcaggtca ttggtgatac
    16561 cactgcggtg cgcctgaatg tgatgggcga aaaaacgcac gatgccggac gcgacaaagt
    16621 caaaaatgag cgttacggcg tcgccccttc tatcgctttt ggccttggta cagcgaatcg
    16681 tttgtatctt aattatctgc atgtcaccca gcacaacacg ccagacggcg gcattccgac
    16741 catcggtttg ccgggttatt ctgccccatc tgcgggaaca gcggctctga atcattccgg
    16801 aaaagttgat actcataact tttacggcac ggattccgat tacgacgatt cgaccaccga
    16861 caccgccacc atgcgttttg agcacgacat caacgataac accaccattc gcaatactac
    16921 ccgttggtcg cgcgtaaagc aggattacct gatgacggcg attatgggcg gggcgtcgaa
    16981 tattactcag cccaccagcg atgtgaatag ctggacctgg tcacgcacgg cgaataccaa
    17041 agatgtgagt aataaaattc tcaccaacca gaccaacctg acctcgacgt tctataccgg
    17101 ttctatcggt catgatgtca gtaccggcgt ggaatttacc cgtgaaacgc agactaacta
    17161 cggcgttaat ccggtgacgt tacccgcggt aaatatttat catcctgaca gcagcattca
    17221 tcccggcggc ctgacgcgca acggcgcaaa cgccaatggt cagacggata ccttcgcaat
    17281 ttacgccttc gatacgctgc aaatcacccg tgattttgag ctgaacggcg ggatccgtct
    17341 ggataattat catactgaat atgacagtgc caccgcctgc ggcggcagcg gacgcggtgc
    17401 catcacctgc ccaactggtg tggcaaaagg ttctccggtc accaccgtcg acaccgccaa
    17461 gtcgggcaat ctggtgaact ggaaagccgg ggcgctgtat cacctgacgg aaaacggtaa
    17521 tgtctatatt aactatgccg tttcccagca gcctccgggc ggcaacaact tcgcccttgc
    17581 gcagtctggc agcggtaaca gtgccaaccg caccgatttt aaaccacaaa aagccaacac
    17641 cagcgagatt ggcaccaaat ggcaggttct ggataaacgc ctgttgctca ccgccgcgct
    17701 attccgtact gatatcgaaa atgaagttga gcaaaatgat gacggaactt actcgcaata
    17761 cggtaagaaa cgtgtcgaag gctatgagat atccgtggcc gggaatatca cgcccgcgtg
    17821 gcaggtgatt ggcggctata cccagcaaaa agcaaccatc aaaaacggca aagatgttgc
    17881 ccaggatggt tcctcatcgc tgccgtatac cccggagcac gccttcacct tatggagcca
    17941 gtatcaggca acagatgata tctctgttgg cgcgggcgca cgctatatcg gcagtatgca
    18001 taaaggttca gacggcgcgg tgggaacgcc agcgtttacc gaaggttact gggtcgccga
    18061 tgccaaactg gggtatcggg ttaatcgcaa tctcgacttc cagctaaacg tttacaacct
    18121 gtttgatacc gattacgtcg cctcaatcaa caagagcggc taccgttatc acccgggcga
    18181 gccaagaacc ttcttgctca cagccaatat gcatttctga ttcagatgtg gggcgcaggc
    18241 cccacttttt ggagaacttg tatgatgtac cacattcccg gcgtgttatc gccacaggac
    18301 gtcgcccggt ttcgcgaaca actggaacaa gccgaatggg tggatggacg cgtcaccacc
    18361 ggcgcacaag gtgcgcaagt taagaacaat caacaggtcg acacccgcag cacgttatac
    18421 gccgccctgc aaaatgaggt gctgaacgcg gttaaccaac atgctttatt ctttgccgcg
    18481 gccttgccgc gtaccctttc cacgccgctg tttaatcgct atcagaacaa tgaaacctat
    18541 ggtttccatg tggatggcgc agtacgcagc catccacaaa acggctggat gcgtactgac
    18601 ctttctgcca cgctgttttt aagcgatcca caaagctacg acggcggcga actggtcgtt
    18661 aatgacacct tcggacaaca tcgggtaaaa ctcccggcag gcgatctcgt gttgtatccc
    18721 tccagcagcc tgcattgcgt gacacccgta acccgcggcg tacgagtggc atcatttatg
    18781 tggatccagt cgatgatccg tgatgataaa aagcgcgcca tgctgtttga actggacaac
    18841 aatattcagt cgctgaaaag ccgctacggc gaaagtgaag agatcctgtc gctgcttaat
    18901 ctttatcata atctgctgcg ggaatggtcg gagatctgat gcttgaactg cccaggttcg
    18961 tccctggatt agctacactt aactgtacaa gcattgatat ggggaggacg atatggcatc
    19021 cggttgggct aacgatgacg ccgtcaacga acagatcaac agtacaattg aagatgcgat
    19081 tgcccgcgct cggggtgaaa ttccgcgtgg cgaaagcctg gatgaatgtg aagagtgcgg
    19141 tgcccccatc ccgcaagccc gtcgggaagc cattcctggc gtgcgcttat gcattcattg
    19201 tcagcaggag aaaggtttac aaaaaccagc gtatacagga tatagtcgca gaggttcgaa
    19261 agacagccag ttacgttaac tacaactggc gagatgcata gcgagtataa tttgtatttt
    19321 gcgtggcatt ccgtgccttt aacgctgctg tgactgccac gcggcataaa acaagtccat
    19381 aagtacaaca aataaatggt ttatcagtat gttagatatc aatcaattta tttgaacaag
    19441 gcggtcaatt ctcttcgatt ttatctctcg taaaaaaacg tgatactcat cacatcgacg
    19501 aaacaacgtc acttatacaa aaatcacctg cgagagatta attatgaaaa ctatcaatac
    19561 tgttgttgct gctatggctc tttcaactct gtcatttggc gtgtttgccg cggaaccggt
    19621 aacggcatcc caggcacaga acatgaataa aatcggcgtg gtttctgccg atggcgcatc
    19681 caccctcgat gccctggaag cgaaactggc tgagaaagcc gcggcagccg gtgccagtgg
    19741 atacagcatc acttccgcca ccaacaacaa taaattaagc ggtactgcgg taatttataa
    19801 gtaattcgta ggacggataa ggcgtttacg ccgcatccgg caacagatgc ctgatgcgac
    19861 gctaccgcgt cttatcagta tctacataat ctctgcaatc tattgaattt gcatgttttt
    19921 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    19981 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    20041 tctctatagc caattgttcg cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    20101 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    20161 gcgcggttgc cagcaagagc gcacgcagtt ctggatgctg ttcgaattta gcgcgaagtg
    20221 cttttcgcat cacctgttct ttgaccgact cccagttttt acgcagaggc ttagaacgat
    20281 cgcgccccat gcgtgcggcg accatagggg aagaaacccg acggatctct tcgcggtatt
    20341 tttcatcaag gaatttttgt gcctgaaaat agtgttctga ggtgggccag gttttcccgt
    20401 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    20461 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    20521 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    20581 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    20641 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    20701 atccccgact ttattccccg tgcgccgcag cggcagatga ttgcggacgt cgccaaaacg
    20761 ctggccggag aagaagggcg acatctggcg attgaagccc ccaccggcgt tgggaaaacg
    20821 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    20881 agtaccgcca acgtggcatt gcaggatcag atttacagca aagatttacc gctgctgaaa
    20941 aagatcattc ccgatcttaa attcactgcc gcttttgggc gtgggcgcta cgtttgcccg
    21001 cgtaatctga cggcgctcgc cagcactgag cccacgcaac aggatctgct ggcgtttctt
    21061 gacgacgaac tgacgccaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    21121 gatctcgaca cttataaatg ggatggtctg cgcgatcata ctgatatcgc tatagatgac
    21181 gatctctggc gtcgtttaag taccgacaaa gccagctgcc tcaaccgcaa ctgttactac
    21241 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    21301 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactacc tgacccgaaa
    21361 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    21421 gagatgagtg ccgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    21481 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    21541 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    21601 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    21661 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    21721 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccacga tattgtgcgt
    21781 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    21841 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    21901 cgggaagagc gcgaagggca gctgcatctc tggtttcact gcgtgggaat acgtgtcagc
    21961 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    22021 ttgcgttcgc tgaacagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    22081 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    22141 attccccgga tgcgctttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    22201 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    22261 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg cctgatgctg
    22321 ctggtgcagg gcgatcagcc gcgttatcgc ttagttgaac tgcaccgcaa acgcgttgcc
    22381 aacggtgagc gtagtgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    22441 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccg
    22501 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    22561 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    22621 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    22681 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    22741 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    22801 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    22861 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgcccatat gctcaatggt
    22921 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggggaaggg
    22981 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    23041 cctgcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    23101 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    23161 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    23221 acgccaacgt tgcacggcgg acaggcgcag gcgaagctcg acgaacatca accggtgttt
    23281 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    23341 ggcgtgcgta acactgcaca taccctggcg aaactggcaa cgccatttgc cgaaggtgag
    23401 gcgctgcgtc tttccagcgt ttcgcatccg gaatacattg gacgcgtcgc gaagttcttt
    23461 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    23521 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    23581 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    23641 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    23701 atggcttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    23761 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    23821 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    23881 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    23941 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    24001 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    24061 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    24121 accatcatgc caaaaccgtt aaagagtcgg gggcggcggt cacgctcgat ggcgatcgcg
    24181 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcataagc
    24241 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    24301 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    24361 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    24421 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    24481 ttggtaaaac tcgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    24541 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggggcgc
    24601 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    24661 gggcgctttc tggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    24721 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    24781 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccgcatgat gatgataagc
    24841 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    24901 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    24961 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    25021 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    25081 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    25141 ggttcggatt ttgtagatac tgataagcgt agcgcatcag gcagttttgc gtttgttcat
    25201 cagcctgatg ccgggcatag cccggcataa aagattactg cgcagcggta ggtttacgcg
    25261 gacggcggcg acgttgttgt tcgcctgccg gtttggcatc gccgaggcgg cgagacggtt
    25321 tttctgcagg tttcgcgctt gcagattttg cgccaccttc cccacggcgt ggttgctgtt
    25381 gaccgcgacc accaccttgc ccacgaccgc cgtcgccacg ttgctggcga ccgttctgga
    25441 tcggttcggc tttgattgac gggtccggct catagcccgg aatcgcaatg cgcgggatct
    25501 cttttttcag cagtttttcg atatcacgca gcagtttgtg ttcatcaaca cacaccagcg
    25561 acaacgcttc accggtagca gccgcacgac cggtacgccc gatacggtgg acataatctt
    25621 ccggtacgtt tggcagttca tagttgacga cgtgcggcaa ctcttcgata tccagaccgc
    25681 gcgcagcgat atcggtcgcc accagtacgc gaatgtcacc cgatttaaaa tcagccagcg
    25741 cacgggtacg cgcaccttgc gatttattcc cgtggatcgc cgcactacgg atgccatctt
    25801 tattgagctg ctcagccaga tggttagcgc cgtgtttggt acgggtaaac accagcacct
    25861 gctgccagtt ccctttacca atcatgtgcg acagcaattc gcgtttgcgt ttcttatcaa
    25921 caaagtgaac gtgctgggtc acctgatcag acgcggtatt gcggcgtgcc acttcgattt
    25981 ccagcgggtt gtgcaacagt ttttccgcca gggctttaat atcgtcagag aaggtcgcag
    26041 agaacagcag gttctggcgc ttcgctggca gttttgttaa cacgcgacgg atatcgtgga
    26101 taaagcccat gtcgagcatg cggtcagctt catcgaggac gaggatttca acctgatcca
    26161 gcttcactgc attttgatgt tccaggtcca gcaaacgtcc cggtgttgcc accagaacat
    26221 caacgccgcc gcgcagcttc atcatttgcg ggttaatgct gacaccgcca aacaccacca
    26281 gcgaacgaat gttcaaatat ttgctgtaat cacggacgtt ttcgccaatc tgcgcagcca
    26341 gttcacgggt cggggtaaga atgagcgcac gtaccggacg acgccctttg gcgtgcggct
    26401 ggcgagtgat caggtgttgc aacagcggca gcgtaaagcc cgctgtttta ccggtaccgg
    26461 tctgggcgct agccatcagg tcgcggcctt ccagcaccgc agggatcgcc tgctgctgaa
    26521 taggggtggg ttcacggtaa ccctgctcgg caacggcgcg caggatatca gggcttaaac
    26581 ccaaagaatc gaaagacata actactccga accgccccga ccgttaccgg tgtagttttc
    26641 agggagatac tgaaaaggaa aatgacaaaa accacaatgt catgagggag cggagtgtag
    26701 cagtttttgt gacgcagcgc ataaattatc cctgtgactg gcgtagctaa aattcttagc
    26761 catactggac aactcccata tggagtcata atcttaatca atcatttgat taagaatgga
    26821 ctgtgcgatg aataatcctg ccatgacaat caagggtgaa caggcgaaaa aacagctgat
    26881 tgctgccgca ctggcgcagt ttggtgaata tggaatgaac gccaccaccc gcgagatagc
    26941 cgcccaggcc gggcagaata tcgctgccat cacctactac ttcggttcga aagaagattt
    27001 gtacctcgcc tgcgcccagt ggattgccga ttttattggc gagcagttcc gtccgcacgc
    27061 cgaagaggcc gaacgcttgt tcgcacaacc acagcctgat cgcgcagcca tccgtgaact
    27121 gatccttcgc gcctgcagga acatgattaa gctgctcacc caggatgaca ccgtcaacct
    27181 cagcaagttt atctcccgtg agcagctctc tcccacggca gcctatcacc tggtgcacga
    27241 acaggtgatt agcccgctac acagccacct gacacgcctg attgccgcct ggaccggctg
    27301 cgacgccaac gatacccgca tgatcctcca tactcatgcg ttgattggtg agattctggc
    27361 gttccgtctt ggcaaagaaa caattctgtt acgtaccggc tggaccgcgt tcgatgaaga
    27421 aaagaccgaa ctgatcaacc agacggtgac ctgtcatatc gacctgattt tgcaaggatt
    27481 atcgcaaagg agtttgtagt gatgaaaaaa cctgtcgtga tcggactggc ggtagtggta
    27541 cttgccgccg tggttgccgg aggctactgg tggtatcaaa gccgccagga taacggcctg
    27601 acgctgtatg gcaacgtgga tattcgtacg gtgaatctta gtttccgtgt tggggggcgc
    27661 gttgaatcgc tggcggtgga cgaaggtgat gctatcaaag cgggccaggt gctgggcgaa
    27721 ctggatcaca agccgtatga gattgccctg atgcaggcga aagcgggtgt ttcggtggca
    27781 caggcgcagt atgacctgat gcttgccggg tatcgcgatg aagaaatcgc tcaggccgcc
    27841 gcagcggtga aacaggcgca agccgcctat gactatgcgc agaacttcta taaccgccag
    27901 caagggttgt ggaaaagccg caccatttcg gcaaacgacc tggaaaatgc ccgctcctcg
    27961 cgcgaccagg cgcaggcaac gctgaaatca gcacaggata aattgcgtca gtaccgttcc
    28021 ggtaaccgtg aacaggacat cgcccaggcg aaagccagcc tcgaacaggc gcaggcgcaa
    28081 ctggcgcagg cggagttgaa tttgcaggac tcaacgttga tagccccgtc tgatggcacg
    28141 ctgttaacgc gcgcggtgga gccaggcacg gtcctcaatg aaggtggcac ggtgtttacc
    28201 gtttcactaa cgcgtccggt gtgggtgcgc gcttatgttg atgaacggaa tcttgaccag
    28261 gcccagccgg ggcgcaaagt gctgctttat accgatggtc gcccggacaa gccgtatcac
    28321 gggcagattg gtttcgtttc gccgactgct gaatttaccc cgaaaaccgt cgaaacaccg
    28381 gatctgcgta ccgacctcgt ctatcgcctg cgtattgtgg tgaccgacgc cgatgatgcg
    28441 ttacgccagg gaatgccggt gacggtgcaa ttcggtaacg aggcaggaca tgaatgatgc
    28501 cgttatcacg ctgaacggcc tggaaaaacg ctttccgggc atggacaagc ccgccgtcgc
    28561 gccgctcgat tgtaccattc acgccggtta tgtgacgggg ctggtggggc cggatggtgc
    28621 aggtaaaacc acgctaatgc ggatgctggc gggattactg aaacccgaca gcggcaatgc
    28681 cacggtgatt ggctttgatc caatcaaaaa cgacggcgcg ctgcacgccg tgctcggcta
    28741 tatgccgcag aaatttggtc tgtatgaaga tctcacggtg atggagaacc tcaatctgta
    28801 cgcggatttg cgcagcgtca ccggcgaggc acgtaagcaa acttttgctc gcctgctgga
    28861 gtttacgtct cttgggccgt ttaccggacg cctggcgggc aagctctccg gtgggatgaa
    28921 acaaaaactc ggtctggcct gtaccctggt gggcgaaccg aaagtgttgc tgctcgatga
    28981 acccggcgtc ggcgttgacc ctatctcacg gcgcgaactg tggcagatgg tgcatgagct
    29041 ggcaggcgaa gggatgttaa tcctctggag tacctcgtat ctcgacgaag ccgagcagtg
    29101 ccgtgacgtg ttactgatga acgaaggcga gttgctgtat cagggggaac caaaagccct
    29161 gacacaaacc atggccggac gcagttttct gatgaccagt ccacacgagg gcaaccgcaa
    29221 actattgcaa cgcgccttga aactgccaca ggtcagcgac ggcatgattc aggggaaatc
    29281 ggtacgtctg atcctcaaaa aagaggccac accagacgat attcgccatg ccgacgggat
    29341 gccggaaatc aacatcaacg aaactacgcc gcgttttgaa gatgcattta ttgatttgct
    29401 gggtggtgcc ggaacctcag aatcgccgct gggcgcaata ttacatacgg tagaaggcac
    29461 acccggcgag acggtgatcg aagcgaaaga actgaccaag aaatttggtg attttgccgc
    29521 caccgatcac gtcaactttg ccgttaaacg tggggagatt tttggtttgc tggggccaaa
    29581 cggcgcgggt aaatcgacca cctttaagat gatgtgcggt ttgctggtgc cgacttccgg
    29641 ccaggcgctg gtgctgggga tggatctgaa agagagttct ggtaaagcgc gccagcatct
    29701 cggctatatg gcgcaaaagt tttcgctcta cggtaacctg acggtcgaac agaatttacg
    29761 ctttttctct ggtgtgtatg gcttacgcgg tcgggcgcag aacgaaaaaa tctcccgtat
    29821 gagcgaggcg ttcggcctga aaagtatcgc ctcccacgcc accgatgaac tgccattagg
    29881 ttttaaacag cggctggcac tggcctgctc gctgatgcat gaaccggaca ttctgtttct
    29941 cgacgaaccg acgtccggcg ttgatccgat tactcgccgc gaattctggc tacatatcaa
    30001 cagcatggta gagaaaggcg tcacggtgat ggtcaccacc cactttatgg atgaagcaga
    30061 atattgcgac cgcatcggcc tggtgtaccg tgggaaatta attgccagcg gcacgccgga
    30121 tgatttgaaa gcgcagtcgg ccaacgacga gcaacccgat cccaccatgg agcaagcctt
    30181 tattcagttg atccacgact gggataagga gcatagcaat gagtaacccg atcctgtcct
    30241 ggcgtcgcgt acgggcgctg tgcgttaaag agacgcggca gatcgttcgc gatccgagta
    30301 gctggctgat tgcggtagtg atcccgctgc tactgctgtt tatttttggt tacggcatta
    30361 acctcgactc cagcaagctg cgggtcggga ttttactgga acagcgtagc gaagcggcgc
    30421 tggatttcac ccacaccatg accggttcgc cctacatcga cgccaccatc agcgataacc
    30481 gtcaggaact gatcgccaaa atgcaggcgg ggaaaattcg cggtctggtg gttattccgg
    30541 tggattttgc ggaacagatg gagcgcgcca acgccaccgc accgattcag gtgatcaccg
    30601 acggcagtga gccgaatacc gctaactttg tacaggggta tgtcgaaggg atctggcaga
    30661 tctggcaaat gcagcgagcg gaggacaacg ggcagacttt tgaaccgctt attgatgtac
    30721 aaacccgcta ctggtttaac ccggcggcga ttagccagca cttcattatc cccggtgcgg
    30781 tgaccattat catgacggtc atcggcgcga ttctcacctc gctggtggtg gcgcgagaat
    30841 gggaacgcgg caccatggag gctctgctct ctacggagat tacccgcacg gaactgctgc
    30901 tgtgtaagct gatcccttat tactttctcg ggatgctggc gatgttgctg tgtatgctgg
    30961 tgtcagtgtt tattctcggc gtgccgtatc gcgggtcgct gctgattctg ttttttatct
    31021 ccagcctgtt tttactcagt accctgggga tggggctgct gatttccacg attacccgca
    31081 accagttcaa tgccgctcag gtcgccctga acgccgcttt tctgccgtcg attatgcttt
    31141 ccggctttat ttttcagatc gacagtatgc ccgcggtgat ccgcgcggtg acgtacatta
    31201 ttcccgctcg ttatttcgtc agcaccctgc aaagcctgtt cctcgccggg aatattccag
    31261 tggtgctggt ggtaaacgtg ctgtttttga tcgcttcggc ggtgatgttt atcggcctga
    31321 cgtggctgaa aaccaaacgt cggctggatt agggagaaga gcatgtttca tcgcttatgg
    31381 acgttaatcc gcaaagagtt gcagtcgctg ctgcgcgaac cgcaaacccg cgcgattctg
    31441 attttacctg tgcttattca ggtgattctg ttcccgttcg ccgccacgct ggaagtgact
    31501 aacgccacca tcgccatcta cgatgaagat aacggcgagc attcggtgga gctgacccaa
    31561 cgttttgccc gcgccagcgc ctttactcat gtgctgctgc tgaaaagccc acaggagatc
    31621 cgcccaacca tcgacacaca aaaggcgtta ctactggtgc gtttcccggc tgacttctcg
    31681 cgcaaactgg ataccttcca gaccgcgccg ttgcagttga tcctcgacgg gcgtaactcc
    31741 aacagtgcgc aaattgccgc caactacctg caacagatcg tcaaaaatta tcagcaggag
    31801 ctgctggaag gaaaaccgaa acctaacaac agcgagctgg tggtacgcaa ctggtataac
    31861 ccgaatcttg actacaaatg gtttgtggtg ccgtcgctga tcgccatgat caccactatc
    31921 ggcgtaatga tcgtcacttc actttccgtc gcccgcgaac gtgaacaagg tacgctcgat
    31981 cagctactgg tttcgccgct caccacctgg cagatcttca tcggcaaagc cgtaccggcg
    32041 ttaattgtcg ccaccttcca ggccaccatt gtgctggcga ttggtatctg ggcgtatcaa
    32101 atccccttcg ccggatcgct ggcgctgttc tactttacga tggtgattta cggtttatcg
    32161 ctggtgggat tcggtctgtt gatttcatca ctctgctcga cacaacagca ggcgtttatc
    32221 ggcgtgtttg tttttatgat gcccgccatt ctcctttccg gttacgtttc tccggtggaa
    32281 aacatgccag tatggctgca aaacctgacg tggattaacc ctattcgcca ctttacggac
    32341 attaccaagc agatttattt gaaggatgcg agtctggata ttgtgtggaa tagtttgtgg
    32401 ccgctactgg tgataacggc cacgacaggg tcagcggcgt acgcgatgtt tagacgtaag
    32461 gtgatgtaac ttcttatctt tcgccagcaa agacactacc gccgggccgg caaggattgc
    32521 cagccctgca attgccagca aaaagttgtt ttccagtaca cgggacaaca gccacagcac
    32581 aatcagcgct gcgccaaaat accaggtcgt ggtcagctct tccagcacgt ctgccacctc
    32641 acgccagcgt tgctcggggt ggcgctgaaa caccaacatc atcactaccg tgacacaata
    32701 cagtgcgcat aatcccacgc cgacgtgaac cagagtctta ctcatccagc ccaccaccag
    32761 cagcgccact accagtaaat gcaacataat ctgccagcaa cttagaccag ttgcgacacg
    32821 aacacgttgt tgccacttca tggcttctct cctgaagaat ttatctctac ttactcagag
    32881 taccgaactt tacggaaaat tccgtaagac cgcacctttt tgtgacgccg actacactat
    32941 ttcaatgaga taagaggcaa agacaggaaa attatgcccg atcaaacaca acaattttcg
    33001 ttcaaggtgc tcaccatcaa cattcacaaa ggttttaccg cgtttaaccg acgcttcatt
    33061 ttgccggaac ttcgcgacgc ggtgcgtacc gtcagcgccg atattgtttg cctgcaggaa
    33121 gtgatgggcg cgcacgaagt tcatccgctg catgtggaaa actggcccga tacctcgcac
    33181 tacgagtttc tcgccgacac gatgtggagc gattttgcct acggtcgcaa tgccgtatac
    33241 ccgaaagggc atcacggcaa cgccgtactg tcgcgttatc ccattgaaca ttatgagaat
    33301 cgcgatgttt cggtcgatgg tgcggaaaag cgcggcgtgc tctactgccg cattgtgcca
    33361 ccgatgaccg gaaaagcgat tcatgtgatg tgcgtacatc tgggcctgcg tgaggcgcac
    33421 cgtcaggcgc agcttgcgat gctcgccgaa tgggtgaatg agctaccgga cggcgaaccg
    33481 gtattggtgg cgggtgattt caatgactgg cggcaaaaag ctaatcatcc gttaaaagtg
    33541 caggccggac tggatgagat ttttacccgc gcccacggac gcccggcgcg cacgtttccg
    33601 gtgcaatttc ctctactacg actggacagg atctacgtca aaaatgccag cgccagcgcg
    33661 ccaaccgcgt tgccgctgcg gacatggcga cacctttctg atcatgcccc tttaagtgcg
    33721 gagattcatt tatgaaatgt agctggcgcg aaggcaataa gatccagttg ctggaaaacg
    33781 gcgagcaata ttatcccgcg gtgtttaagg cgattggcga ggcacaagaa cgcatcattc
    33841 ttgaaacgtt tatctggttt gaggatgacg tcggcaaaca gctgcatgcg gcactactgg
    33901 cagcagcgca acgcggggtt aaagcggaag tcttgctgga tggctacggt tcgccggatc
    33961 tcagcgatga gtttgtcaat gaactgacgg cagctggcgt ggtatttcgc tactacgatc
    34021 cccgccctcg cctttttggt atgcgcacca atgtgtttcg ccggatgcat cgcaaaattg
    34081 tggtgatcga cgcgcgtatc gcctttattg gcgggctgaa ttactccgcc gagcatatgt
    34141 ccagctacgg tccagaggct aaacaggatt acgcggtacg ccttgaaggg ccgattgtcg
    34201 aagatattct ccagtttgag ctggaaaacc tgcctggaca gagcgcggca cgacgctggt
    34261 ggcgacgtca tcacaaagcg gaagagaacc gccagccggg agaagcgcag gtattgctgg
    34321 tctggcgcga taacgaagaa catcgcgatg atattgaacg tcactatctg aaaatgctca
    34381 ctcaggcgcg gcgagaagtc attatcgcca acgcctactt cttccccggc tatcgatttt
    34441 tacacgcctt gcgtaaagcg gcacggcgcg gggtgcggat caaactgatc attcagggcg
    34501 aaccggatat gccgattgtc agagtcggtg cgcgcttgct gtataactat ctggttaaag
    34561 gcggcgttca ggtatttgag taccgccgcc gcccgctcca cggcaaagtg gcattgatgg
    34621 acgatcactg ggcgacagta gggtccagta atctcgatcc gctcagtttg tcactgaatc
    34681 tcgaagcaaa tgtcatcatc cacgatcgtc attttaacca gacgctgcgc gataatctga
    34741 acggcattat tgccgcagat tgtcagcagg tcgatgagac catgctgccg aaacgcacct
    34801 ggtggaacct gaccaaaagc gtgctggcgt tccacttttt acgccacttc ccggcgctgg
    34861 ttggctggct tccggcacac acaccacgtc tggcgcaggt tgatccgccc gcacaaccga
    34921 caatggaaac gcaggatcgg gtagaaactg aaaacacggg ggtaaaaccc tgatgagtaa
    34981 atcacacccg cgctggcgct tagcaaagaa gatcctcacc tggctgtttt ttatcgcggt
    35041 gattgtgttg ctggtggtct acgccaaaaa agtggactgg gaagaggtct ggaaggtcat
    35101 ccgcgactac aatcgcgttg cgctgcttag tgcggtcggg ctggtggtcg tcagctatct
    35161 gatttacggc tgctatgacc tgctcgcccg cttttactgc ggtcacaaac tggcgaagcg
    35221 ccaggtgatg ctggtgtcgt ttatctgcta cgccttcaac ctgacgctca gtacctgggt
    35281 cggcggcatt ggtatgcgct atcgtttgta ctcccgacta gggttaccgg gcagcactat
    35341 tacgcggatt ttctcgctca gtattaccac caactggctg ggctatattt tactggcagg
    35401 gattatcttt accgcaggcg tggtggagtt gccggatcac tggtatgtcg atcaaactac
    35461 gctgcgtatt ctcggcattg gcttactgat gattatcgcg gtttatttgt ggttttgcgc
    35521 tttcgcgaag caccgccata tgaccatcaa agggcaaaag ctggtgctgc cttcatggaa
    35581 attcgccctc gctcagatgc tgatttccag cgttaactgg atggtaatgg gggcgattat
    35641 ctggctgtta cttggtcaaa gcgtgaacta tttctttgta ctgggcgtgt tactggttag
    35701 tagtattgct ggcgtcatcg tgcatattcc ggtggggatc ggtgtgctgg aagcggtgtt
    35761 tatcgcacta ctggctgggg agcatacctc gaaagggagc attatcgccg ccctgctcgc
    35821 ttatcgcgtc ctgtattact ttatcccgct gctgctggcg ctgatctgtt acctgctact
    35881 ggaaagccag gcgaagaagc tacgggcgaa aaatgaagcg gcgatgtgaa gtttagttaa
    35941 gttctttagt atctgcattt acgactaatg aaaaaaacgc gtatgccttt gccagacaag
    36001 cgttatagct atcgcggcaa gtatcccaaa gaagccgata aaatagtaat agagtgttaa
    36061 tgcacctgta acagcgcaac gaaccggact ccacaactct tcactatggc atttccccgc
    36121 caattcaagg agcttatttc gtccatgcga gataatgcct gaccataaca caatcattaa
    36181 gcaacttatt acccaaacgg gtcgttcact cattaatgcg gcattcatga tgatcaccag
    36241 ggccaaccct gtcaacgtca tcataatgat aaaacggtga gaaccaggat caacattaaa
    36301 taataaaccg gccagcatac tgattaaaaa catcgcgccc gcagtgctga acacgacgat
    36361 aatggcaggt acaggagcta aacttgcaac gacaatcgcc accaaagaga gtaaaaacag
    36421 atatattacg cctgcgatga cggcatttat cttgttgata tccttactta agccatacat
    36481 acaaactaat aataacccta tggcgacata cattgttggt gaatcatatt tggttgccgt
    36541 aatttcgaga tacttagctc cccaggccag tcctgcggtt gccaccaaac ctaaagcggt
    36601 aatgagccat ataccgttta atatcctggt ctcgtgctga aaatctaatt tattgctgtt
    36661 ttgtgagtat gattccattc ttttagttgt ccttagaaaa aatattaata ttcaccatca
    36721 ataa
//
LOCUS       DAZFWL020000037        35475 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFWL020000037 DAZFWL020000000
VERSION     DAZFWL020000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.037"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            44..586
                     /locus_tag="ACHKNP_RS09705"
                     /old_locus_tag="pgaptmp_001941"
     CDS             44..586
                     /locus_tag="ACHKNP_RS09705"
                     /old_locus_tag="pgaptmp_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4704114.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAAKKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            669..1370
                     /gene="elfD"
                     /locus_tag="ACHKNP_RS09710"
                     /old_locus_tag="pgaptmp_001942"
     CDS             669..1370
                     /gene="elfD"
                     /locus_tag="ACHKNP_RS09710"
                     /old_locus_tag="pgaptmp_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4704115.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1395..3995
                     /gene="elfC"
                     /locus_tag="ACHKNP_RS09715"
                     /old_locus_tag="pgaptmp_001943"
     CDS             1395..3995
                     /gene="elfC"
                     /locus_tag="ACHKNP_RS09715"
                     /old_locus_tag="pgaptmp_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGU4704116.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            3986..5056
                     /gene="elfG"
                     /locus_tag="ACHKNP_RS09720"
                     /old_locus_tag="pgaptmp_001944"
     CDS             3986..5056
                     /gene="elfG"
                     /locus_tag="ACHKNP_RS09720"
                     /old_locus_tag="pgaptmp_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4704117.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNEGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5068..5610
                     /gene="ycbU"
                     /locus_tag="ACHKNP_RS09725"
                     /old_locus_tag="pgaptmp_001945"
     CDS             5068..5610
                     /gene="ycbU"
                     /locus_tag="ACHKNP_RS09725"
                     /old_locus_tag="pgaptmp_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4704118.1"
                     /translation="MKKKTIYQYVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5618..6133
                     /gene="ycbV"
                     /locus_tag="ACHKNP_RS09730"
                     /old_locus_tag="pgaptmp_001946"
     CDS             5618..6133
                     /gene="ycbV"
                     /locus_tag="ACHKNP_RS09730"
                     /old_locus_tag="pgaptmp_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4704119.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6099..6836
                     /gene="ycbF"
                     /locus_tag="ACHKNP_RS09735"
                     /old_locus_tag="pgaptmp_001947"
     CDS             6099..6836
                     /gene="ycbF"
                     /locus_tag="ACHKNP_RS09735"
                     /old_locus_tag="pgaptmp_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU4704120.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6947..7957
                     /gene="pyrD"
                     /locus_tag="ACHKNP_RS09740"
                     /old_locus_tag="pgaptmp_001948"
     CDS             6947..7957
                     /gene="pyrD"
                     /locus_tag="ACHKNP_RS09740"
                     /old_locus_tag="pgaptmp_001948"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HGU4704121.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8131..8673
                     /gene="zapC"
                     /locus_tag="ACHKNP_RS09745"
                     /old_locus_tag="pgaptmp_001949"
     CDS             8131..8673
                     /gene="zapC"
                     /locus_tag="ACHKNP_RS09745"
                     /old_locus_tag="pgaptmp_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HGU4704122.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8670..9779)
                     /gene="ycbX"
                     /locus_tag="ACHKNP_RS09750"
                     /old_locus_tag="pgaptmp_001950"
     CDS             complement(8670..9779)
                     /gene="ycbX"
                     /locus_tag="ACHKNP_RS09750"
                     /old_locus_tag="pgaptmp_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HGU4704123.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PGAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10023..12131
                     /gene="rlmKL"
                     /locus_tag="ACHKNP_RS09755"
                     /old_locus_tag="pgaptmp_001951"
     CDS             10023..12131
                     /gene="rlmKL"
                     /locus_tag="ACHKNP_RS09755"
                     /old_locus_tag="pgaptmp_001951"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HGU4704124.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLGGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12143..14050
                     /gene="uup"
                     /locus_tag="ACHKNP_RS09760"
                     /old_locus_tag="pgaptmp_001952"
     CDS             12143..14050
                     /gene="uup"
                     /locus_tag="ACHKNP_RS09760"
                     /old_locus_tag="pgaptmp_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4704125.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14180..15433
                     /gene="pqiA"
                     /locus_tag="ACHKNP_RS09765"
                     /old_locus_tag="pgaptmp_001953"
     CDS             14180..15433
                     /gene="pqiA"
                     /locus_tag="ACHKNP_RS09765"
                     /old_locus_tag="pgaptmp_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HGU4704126.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15438..17078
                     /gene="pqiB"
                     /locus_tag="ACHKNP_RS09770"
                     /old_locus_tag="pgaptmp_001954"
     CDS             15438..17078
                     /gene="pqiB"
                     /locus_tag="ACHKNP_RS09770"
                     /old_locus_tag="pgaptmp_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HGU4704127.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17075..17638
                     /gene="pqiC"
                     /locus_tag="ACHKNP_RS09775"
                     /old_locus_tag="pgaptmp_001955"
     CDS             17075..17638
                     /gene="pqiC"
                     /locus_tag="ACHKNP_RS09775"
                     /old_locus_tag="pgaptmp_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HGU4704128.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASAWSQEAASIAQEIKRLP"
     gene            17894..18061
                     /gene="rmf"
                     /locus_tag="ACHKNP_RS09780"
                     /old_locus_tag="pgaptmp_001956"
     CDS             17894..18061
                     /gene="rmf"
                     /locus_tag="ACHKNP_RS09780"
                     /old_locus_tag="pgaptmp_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HGU4704129.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18131..18649)
                     /gene="fabA"
                     /locus_tag="ACHKNP_RS09785"
                     /old_locus_tag="pgaptmp_001957"
     CDS             complement(18131..18649)
                     /gene="fabA"
                     /locus_tag="ACHKNP_RS09785"
                     /old_locus_tag="pgaptmp_001957"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HGU4704130.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18718..20478)
                     /gene="ycbZ"
                     /locus_tag="ACHKNP_RS09790"
                     /old_locus_tag="pgaptmp_001958"
     CDS             complement(18718..20478)
                     /gene="ycbZ"
                     /locus_tag="ACHKNP_RS09790"
                     /old_locus_tag="pgaptmp_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4704131.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLSDFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20664..21116
                     /gene="matP"
                     /locus_tag="ACHKNP_RS09795"
                     /old_locus_tag="pgaptmp_001959"
     CDS             20664..21116
                     /gene="matP"
                     /locus_tag="ACHKNP_RS09795"
                     /old_locus_tag="pgaptmp_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HGU4704132.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21191..22231)
                     /gene="ompA"
                     /locus_tag="ACHKNP_RS09800"
                     /old_locus_tag="pgaptmp_001960"
     CDS             complement(21191..22231)
                     /gene="ompA"
                     /locus_tag="ACHKNP_RS09800"
                     /old_locus_tag="pgaptmp_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HGU4704133.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22588..23097)
                     /gene="sulA"
                     /locus_tag="ACHKNP_RS09805"
                     /old_locus_tag="pgaptmp_001961"
     CDS             complement(22588..23097)
                     /gene="sulA"
                     /locus_tag="ACHKNP_RS09805"
                     /old_locus_tag="pgaptmp_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HGU4704134.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23316..23945
                     /gene="sxy"
                     /locus_tag="ACHKNP_RS09810"
                     /old_locus_tag="pgaptmp_001962"
     CDS             23316..23945
                     /gene="sxy"
                     /locus_tag="ACHKNP_RS09810"
                     /old_locus_tag="pgaptmp_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HGU4704135.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23908..26070)
                     /gene="yccS"
                     /locus_tag="ACHKNP_RS09815"
                     /old_locus_tag="pgaptmp_001963"
     CDS             complement(23908..26070)
                     /gene="yccS"
                     /locus_tag="ACHKNP_RS09815"
                     /old_locus_tag="pgaptmp_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HGU4704136.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26080..26526)
                     /gene="yccF"
                     /locus_tag="ACHKNP_RS09820"
                     /old_locus_tag="pgaptmp_001964"
     CDS             complement(26080..26526)
                     /gene="yccF"
                     /locus_tag="ACHKNP_RS09820"
                     /old_locus_tag="pgaptmp_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HGU4704137.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26649..28703
                     /gene="helD"
                     /locus_tag="ACHKNP_RS09825"
                     /old_locus_tag="pgaptmp_001965"
     CDS             26649..28703
                     /gene="helD"
                     /locus_tag="ACHKNP_RS09825"
                     /old_locus_tag="pgaptmp_001965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HGU4704138.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28735..29193)
                     /gene="mgsA"
                     /locus_tag="ACHKNP_RS09830"
                     /old_locus_tag="pgaptmp_001966"
     CDS             complement(28735..29193)
                     /gene="mgsA"
                     /locus_tag="ACHKNP_RS09830"
                     /old_locus_tag="pgaptmp_001966"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HGU4704139.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29289..29951)
                     /gene="yccT"
                     /locus_tag="ACHKNP_RS09835"
                     /old_locus_tag="pgaptmp_001967"
     CDS             complement(29289..29951)
                     /gene="yccT"
                     /locus_tag="ACHKNP_RS09835"
                     /old_locus_tag="pgaptmp_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HGU4704140.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30124..30537
                     /gene="yccU"
                     /locus_tag="ACHKNP_RS09840"
                     /old_locus_tag="pgaptmp_001968"
     CDS             30124..30537
                     /gene="yccU"
                     /locus_tag="ACHKNP_RS09840"
                     /old_locus_tag="pgaptmp_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGU4704141.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30582..30899)
                     /gene="hspQ"
                     /locus_tag="ACHKNP_RS09845"
                     /old_locus_tag="pgaptmp_001969"
     CDS             complement(30582..30899)
                     /gene="hspQ"
                     /locus_tag="ACHKNP_RS09845"
                     /old_locus_tag="pgaptmp_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HGU4704142.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30957..32147)
                     /gene="rlmI"
                     /locus_tag="ACHKNP_RS09850"
                     /old_locus_tag="pgaptmp_001970"
     CDS             complement(30957..32147)
                     /gene="rlmI"
                     /locus_tag="ACHKNP_RS09850"
                     /old_locus_tag="pgaptmp_001970"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HGU4704143.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEIFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            32242..32520
                     /gene="yccX"
                     /locus_tag="ACHKNP_RS09855"
                     /old_locus_tag="pgaptmp_001971"
     CDS             32242..32520
                     /gene="yccX"
                     /locus_tag="ACHKNP_RS09855"
                     /old_locus_tag="pgaptmp_001971"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGU4704144.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMLWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32517..32846)
                     /gene="tusE"
                     /locus_tag="ACHKNP_RS09860"
                     /old_locus_tag="pgaptmp_001972"
     CDS             complement(32517..32846)
                     /gene="tusE"
                     /locus_tag="ACHKNP_RS09860"
                     /old_locus_tag="pgaptmp_001972"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HGU4704145.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32937..33596)
                     /gene="yccA"
                     /locus_tag="ACHKNP_RS09865"
                     /old_locus_tag="pgaptmp_001973"
     CDS             complement(32937..33596)
                     /gene="yccA"
                     /locus_tag="ACHKNP_RS09865"
                     /old_locus_tag="pgaptmp_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HGU4704146.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(34004..35023)
                     /locus_tag="ACHKNP_RS09870"
                     /old_locus_tag="pgaptmp_001974"
     CDS             complement(34004..35023)
                     /locus_tag="ACHKNP_RS09870"
                     /old_locus_tag="pgaptmp_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4704147.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(35001..35243)
                     /locus_tag="ACHKNP_RS09875"
                     /old_locus_tag="pgaptmp_001975"
     CDS             complement(35001..35243)
                     /locus_tag="ACHKNP_RS09875"
                     /old_locus_tag="pgaptmp_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGU4704148.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(35311..>35475)
                     /locus_tag="ACHKNP_RS09880"
                     /old_locus_tag="pgaptmp_001976"
     CDS             complement(35311..>35475)
                     /locus_tag="ACHKNP_RS09880"
                     /old_locus_tag="pgaptmp_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4704149.1"
                     /translation="DVRTMVALGLVMDFDARTTIPFEGERHNALNDARYQAKYVSAIW
                     QKLLPSQADF"
BASE COUNT         9122 a         8449 c         9191 g         8713 t
ORIGIN      
        1 ttaaaggcat tatcatattt acgaagagat caggatgatg acgatgaaaa aaagtgtatt
       61 gacggcgttt ataactgtgg tatgtgcaac gtccagcgtt atggcagctg atgataatgc
      121 tatcacggat ggcaaagtaa catttaatgg taaagttatt gctccagctt gtaccctggt
      181 agctgcgaag aaagattcag tagtcacatt gccaaatgtc agcgccacca agttgcaaac
      241 taatggagca gtctctggcg ttaaaactga tgtaccaatt gccttagaag gctgtgatgt
      301 tactgtaact aaaaatgcga cgtttacctt tagtggtacg gctgacggcg tgcaacctac
      361 agcatttgcc aaccaggcta ccaccgatgc agcgacgaat gttgctctac aaatgtatct
      421 gccagatggt tctacatcgg taactcccgg taccgaaacg tcgaatattc agttagcgga
      481 ttctgctgag caaacagtga cctttaaagt tgattatatc gctacgggga aagcgacttc
      541 tggtaatgtg aatgcggtaa caaatttcca tattaactat tattaataga actcattaat
      601 tgttttatta attagtaccc ctctagtgtt ctggagggga tattcatatt ttttaagagt
      661 gactatttat gaaaacttgc ataacaaagg gaattgtgac cgtaagttta acggcaatat
      721 tactcagctg ctcatccgca tgggcggcag gtaaaggcgg gattggactt gcagcaacac
      781 gtcttgttta ttcagaaggt gaagagcaaa tttcactggg tgtacgtaac accagtccgg
      841 atgttcctta tcttattcag tcatgggtga tgaccccaga taataaaaaa tcagcagatt
      901 ttattattac accaccgcta tttgtgctga atccggctaa tgagaatctg ttacgcatta
      961 tgtacattgg tgcgccgttg gcgaaagaca gagaaaccct tttcttcact agcgtacggg
     1021 cagtcccttc aacaacgaag cgggaagagg gaaataccct gaagattgcc acacaaagcg
     1081 tcatcaaact tttctggcga ccaaaaggtt tagcgtatcc cttaggcgag gctccggcga
     1141 aactgcgttg cacttcgtca gctgacatgg ttacggtcag taacccaaca ccttatttca
     1201 ttaccctgac agacctgaaa ataggtggaa aagtagttaa aaatcaaatg atttccccct
     1261 ttgataaata ccaattttct ctgccaaagg gggccaaaaa tagcagcgta acgtatcgaa
     1321 ccatcaatga ctacggggcg gaaacgccgc aactcaactg taaatcgtaa gccgtcttca
     1381 gttaagagag cgagatgtat agaactcatc gacaacacag cctgttaagc tctggtggag
     1441 tgccatcgtt tattggtggg ctggtggtgt ttgtgtcggc agcgttcaat gcacaagctg
     1501 aaacctggtt cgatcctgct tttttcaaag atgatccctc aatggtggcc gatttgtctc
     1561 gtttcgaaaa aggacaaaaa ataacgccag gggtttatcg tgtcgatatt gttctgaatc
     1621 agacaattgt agatacgcgc aacgtcaatt ttgttgagat aacgccagag aaggggattg
     1681 ccgcctgttt gacgactgaa agcctggatg caatgggtgt taatactgat gcgtttccgg
     1741 cttttaaaca actggacaaa caagcgtgtg tgccattggc ggagattatt ccggatgcca
     1801 gcgtaacgtt taatgtgaat aaactccgtc tggaaatttc agtaccgcaa atcgccatca
     1861 aaagtaatgc ccgtggttat gtcccccctg aacgttggga tgaagggatc aacgcgctat
     1921 tactgggata ttcgtttagc ggggctaaca gtattcatag cagcgcagac agtgattctg
     1981 gcgacagcta ttttctgaat ttaaacagtg gcgttaattt aggcccatgg agattgcgca
     2041 acaattcaac atggagtcgc agtagtggcc aaaccgcaga atggaagaat ctcagcagct
     2101 atttgcagcg ggcggttatt ccactaaaag gcgaactgac cgtaggtgat gattatactg
     2161 caggcgattt tttcgatagt gtcagctttc gtggtgtgca gctggcgtca gatgacaaca
     2221 tgctgccaga cagcctgaaa gggtttgcgc ctgtggtgcg tggtatcgcc aaaagcaatg
     2281 cccagataac gattaagcaa aatggttaca ccatttacca aacttatgta tcgcctggtg
     2341 cttttgaaat tagtgatctc tattccacgt cgtcgagcgg tgatttgtta gttgaaatca
     2401 aagaagcgga cggcagcgtc aatagctaca gcgtaccgtt ttccagcgtg ccattactcc
     2461 agcgtcaggg gcgaatcaaa tacgcggtga cactggcgaa atacagaacc aatagtaatg
     2521 aacagcagga gagcaaattt gcccaggcca cgttgcagtg gggcggaccg tggggaacga
     2581 catggtatgg tggtggacaa tatgctgaat attaccgtgc cgccatgttt ggtctgggat
     2641 ttaaccttgg cgatttcgga gcaatttcgt tcgatgcgac ccaggcgaag agtacgctgg
     2701 cagaccaaag cgaacataaa ggtcagtcat atcgttttct gtatgccaaa acgctcaacc
     2761 aattgggcac taattttcaa ttgatgggct atcgctattc gacgtcgggt ttctacaccc
     2821 tttccgacac catgtataaa catatggatg gctacgaatt taatgacggt gatgatgaag
     2881 atacgccgat gtggtcgcgt tattacaatt tgttttacac caaacgtggc aaactgcagg
     2941 tcaatatctc ccagcaatta ggcgagtacg gttcgtttta tttaagtggt agccagcaaa
     3001 cttactggca taccgatcaa caggatcggc tattacagtt tggctacaac acgcaaatta
     3061 aagatctctc gctgggggtt tcctggaact acagtaagtc ccgtggtcaa cctgatgctg
     3121 atcaggtgtt tgcactaaat ttttccctgc cgctcaatct gttgctcccc agaagtaatg
     3181 atagctatac caggaaaaaa aattacgcct ggatgacctc taacaccagt atcgataacg
     3241 aagggcacat tacacaaaac ctgggtttaa cggagacact actcgatgac ggtaacctga
     3301 gctacagcgt gcaacaggga tataacagcg aggggaaaac ggctaatggt agcgccagca
     3361 tggactacaa aggggcgttt gcagatgccc gagtgggcta caactacagc gataacggca
     3421 gtcaacaaca actgaactac gctctttcag gcagtttagt tgcccattca cagggcatta
     3481 ccctggggca atcgctgggg gaaactaacg tgctgattgc agcaccaggc gcagagaata
     3541 ctcgtgtggc gaacagcacc gggctgaaaa ctgactggcg cggatatacc gttgtcccct
     3601 atgccacttc ttatcgggaa aatcgaatcg cacttgatgc ggcatcgtta aaacgtaacg
     3661 tggatcttga aaatgcagta gtcaacgtgg ttcccaccaa aggggcgttg gttctggcgg
     3721 agttcaatgc ccatgcggga gcaagggtat taatgaaaac atcaaagcag ggtataccgc
     3781 tgcgttttgg cgcgatagcg acgctggacg gcatacagac taatagcggc attattgatg
     3841 atgatggctc gctctatatg tccggtttgc ccgctcaggg agcgataact gtacgctggg
     3901 gcgaagctcc cgatcaaatt tgtcatatca gttaccagct taccgaacaa caaattaact
     3961 ctgcgattac gcgaatggat gccatatgca gataatcttt ggagaaaaat gcgtgttatt
     4021 actacgacta ttttttgccg ccgtcttaat gctatggtgc gctcaaactg ctgcttatag
     4081 cgggcagtgt cataccactc aggggaatcc gtatattggc gtcaattttg gcgttaaaac
     4141 cctggaggaa gaagaaaata cggctggagt agttaaagac aaattttatc agtggaacga
     4201 atcgaatgat tattatgttt cctgtgattg cgataaagac aatgtcagaa gtggccgatg
     4261 ggcattcgcc gcggattcac cgttagtcta tttaggcgac aactggtaca aaattaatga
     4321 ctatcttgcc gccaaagttt tattgcaggt taaaggcagt tctcctactg cggttccttt
     4381 cgaaaacgtg ggcacagggg cagatacacg atggcatatt tgcgatcccg gcggtcaacg
     4441 tttaggtggc cagggggcta gcggtaatag cggtagcttt tccctgaaaa tattgcagcc
     4501 gttcgttggt tcggtcgtta ttcctcctat ggcgctggcg cgattatttg aatgctacaa
     4561 catacccgca ggtgattcct gcacgactac aggtacaccg gttttagtgt attacctgtc
     4621 tggtacgatc aattcacttg gctcatgttc cgtcaatgcc ggagagacta tcgaggtcga
     4681 tctgggcgac gtatttgcgg ctaactttcg tgttgtaggg cataaacccc ttggggccag
     4741 aacggcagaa ctcgcaattc cagtcaggtg taacacggga aacgaagggt tagttaacgt
     4801 caacctgagt ctgacggcaa cctcagaccc cagctatccc caggcgatta agacgtcacg
     4861 tcctggcgtg ggcgtggtgg tgaccgatag ccagaacaac attatttccc ctgctggtgg
     4921 gacactaccg ctctctattc ctgatgatgc agacagtatc gcgcgaatga atgtctatcc
     4981 agtcagcacg acaggcgtac caccagaaac cgggcgattt gaagccacgg caacggtgag
     5041 aataaatttt gattaacgcg tgaacgtatg aagaaaaaaa cgatatatca gtacgttatt
     5101 ttgttcttta gccttcttaa catccatgtc gggatggctg ggcctgaaca agtcagtatg
     5161 catatttatg ggaatgtggt cgatcagggc tgtgatgtcg ccaccaaaag tgcattacaa
     5221 aatattcata ttggcgattt taatatcagt gattttcagg ccgcgaatac cgtaagcact
     5281 gcagctgatt tgaatatcga tatcaccggt tgtgccgctg gtattactgg cgcggacgtc
     5341 ctttttagcg gcgaggctga cacccttgcg ccgacactgc ttaaactaac tgacacaggc
     5401 ggaagcggtg gtatggcaac ggggattgcc gtgcaaattc ttgatgcgca aagtcagcaa
     5461 gaaatcccgc tcaatcaggt ccagcctctt acgcccttaa aagccgggga taacacactc
     5521 aaatatcaac ttcgttataa gtccacaaag gcgggagcaa cgggcggtaa tgcgacggcg
     5581 gttctctatt ttgatctggt ttaccagtga aggagtgatg ttgaaacgga taatctggat
     5641 tctgtgctta ctgggattaa cggggagtag tgagttattt gctcatgatg gcacggtcaa
     5701 cattagcgga tcgtttcgtc gtaatacatg cgtgctggca caggatagca agcaaatcaa
     5761 tgtgcagtta ggcgatgtct cgctgacacg tttttctcac gggaattatg gcccggagaa
     5821 atctttcatc attaatttgc aggattgcgg aacagatgtc agcacggttg acgtcacctt
     5881 ttcaggaacc cccgacggag tgcagagcga aatgctgtcc attgaaagtg gtatggatgc
     5941 agccagcggg ctggcaattg cgattctgga tgatgcgaag atattgattc cgcttaacca
     6001 ggcgagtaaa gactacagcc tacatagcgg taaggtgcca ttaacttttt atgcgcaatt
     6061 gcgacctgtc aatagcgatg tgcagtcggg taaagtgaat gccagcgcga catttgtact
     6121 tcattatgac taatacgtgg aacagattgg cgctcttgat tttcgccgtt ttatcgctgc
     6181 tggtggcggg tgaattgcgg gcaggagtgg tggtcggcgg aacgcgattt atctttccgg
     6241 cagacagaga atcgatatct attttactga ctaataccag tcaggaatcc tggcttatta
     6301 acagtaaaat caaccgccca acgcgttggg ccggggggga agcgtcgaca gtgccagcac
     6361 cattactggc cgctccgcca cttattctcc tgaagcccgg tactacaggc acgttgcgct
     6421 tgctgagaac ggaaagcgac atcttgcctg tggatcgcga aacgctattt gagttaagca
     6481 ttgccagcgt gccatccggc aaagttgaaa atcagagcgt aaaagtggcg atgcgctcgg
     6541 tatttaaact gttctggcga cccgaaggtt tgccgggcga cccgctggaa gcttatcaac
     6601 aattacgctg gacacggaat tcgcagggtg tacaactcac taacccaacg ccttactaca
     6661 ttaacctgat tcagttgagc gtaaatggta aagcgttaag taatgcggga gttgtgccgc
     6721 ctaaaagcca gcgtcagaca agctggtgtc aggcaatcgc accgtgtcat gtcgcctggc
     6781 gggcgataaa tgattacggc gggttgagtg caaagaagga gcaaaatctg ccctgaaaca
     6841 ggttcggaaa acgtttgcgt tttttttgcc gcaggtcaat tcccttttgg tccgaactcg
     6901 cacataatac gcccccggtt tgcacaccgg gaatccagga gagttcatgt actacccctt
     6961 cgttcgtaaa gcccttttcc agctcgatcc agagcgcgct catgagttta cttttcagca
     7021 attacgccgt attacaggaa cgccgtttga agcactggtg cggcagaaag tgcctgcgaa
     7081 acctgttaac tgcatgggcc tgacgtttaa aaatccgctt ggtctggcag ccggtcttga
     7141 taaagacggg gagtgcattg acgcgttagg cgcgatggga tttggatcga tcgagatcgg
     7201 taccgtcacg ccacgtccac agccaggtaa tgacaagccg cgtctctttc gtctggtaga
     7261 tgccgaaggt ttgatcaacc gtatgggctt taataatctt ggcgttgata acctcgtaga
     7321 gaacgtaaaa aaggcccatt atgacggcgt cctgggtatt aacatcggca aaaataaaga
     7381 tacgccagtg gagcagggca aagatgacta tctgatttgt atggaaaaaa tctatgccta
     7441 tgcgggatat atcgccatca atatttcatc gccgaatacc ccaggattac gcacgctgca
     7501 atatggtgaa gcgctggatg atctcttaac cgcgattaaa aataagcaaa atgatttgca
     7561 agcgatgcac cataaatatg tgccgatcgc agtgaagatc gcgccggatc tttctgaaga
     7621 agaattgatc caggttgccg atagtttagt tcgccataat attgatggcg ttattgcaac
     7681 caataccaca ctcgatcgtt ctcttgttca gggaatgaaa aattgcgatc aaaccggtgg
     7741 cttaagtggt cgtccgcttc agttaaaaag caccgaaatt attcgccgct tgtcactgga
     7801 attaaacggt cgcttaccga tcatcggtgt tggcggcatc gactcggtta tcgctgcgcg
     7861 tgaaaagatt gctgcgggtg cctcactggt gcaaatttat tctggtttta tttttaaagg
     7921 tccgccgctg attaaagaaa tcgttaccca tatctaatta tttcttcgaa ttcctcttta
     7981 taagataatc agggctttat tttcagccct ggttgtttta tattcatcgc tgttgcttat
     8041 ttagacattt tgtactttta ttgttgaggt tattaagcga agcgacaatg gattgtgttg
     8101 ctgcggtttt atagggtagg ggagaggcag atgcgaatta aaccagacga taactggcgt
     8161 tggtattacg atgaagagca cgatcgaatg atgctcgatt tagccaatgg tatgctattt
     8221 cgctcacgtt ttgcgcgcaa gatgttgact ccagatgcct tttctcccgc aggcttttgc
     8281 gttgacgacg ccgcgctcta tttctctttt gaagaaaagt gccgcgattt taatttatct
     8341 aaagaacaaa aagccgaact ggtgctgaat gcactggtag caattcgcta tctcaaaccg
     8401 caaatgccga aaagctggca ttttgtttcc catggtgaaa tgtgggtgcc catgccgggc
     8461 gatgcggcct gcgtttggtt gagcgatact cacgagcagg tcaatttgct ggtcgttgaa
     8521 tctggcgaaa atgccgcact atgcctgctg gcacaaccct gcgttgttat tgcgggtcgc
     8581 gcgatgcagt tgggcgacgc cattaaaatc atgaacgaca ggctgaaacc gcaggttaat
     8641 gttgacagct tcagcctcga acaggcagtc taacgcgcca acttaagtgc agtcttcggt
     8701 acacagctac agcaaagaat ggtgccatca tcgcccattg ctgatttttt tagcggcgtg
     8761 acttcgcctt ctaaaagctg aacacggcaa cttccacaaa tgcccgcgcg gcaagaataa
     8821 gggatacgaa ttccctgatt ttctaattgc tccagcaaca cctgttggtt atttccacga
     8881 aatgcctgtc cctgccagtc aatatctaca tttgcgtccg gttgttgcgt gatgttggcg
     8941 gtatcatcag cggcagctgc gccgtaaatt tttgccggag ccgttgccag aatttccacc
     9001 tcatcgccaa cgcgaatcac gccgctatta cgggcaatta aattctgacc aaaatcgaca
     9061 tcgccgttat cctgggcagt gcggaaagat tgcaatgttt ttaatggttc gcctgccgga
     9121 tgtttttgcc ctttttctgg gctgacggtg gtgaaaatac agcggctaca aggtttaacc
     9181 acatcaaaca ccacatcacc aatgcgaatc actttccagc gatcttcttc ccaggctgac
     9241 gcgccggaaa ccaccagatt ggggcggaat tgctccattt ttacactggc cggacaacgt
     9301 tgttggagat cacgtaacga ggcttcgtta gcaagaaggt aaggatagcc atcagcaaat
     9361 gacagaggta cagtgttgtg gcgtttcacg cgccgggtca tttgtggccc cacccagcgt
     9421 aattgcactt cgcgggagaa aaatccactt agccatttgt tgatcgcgcc tggcgcaatt
     9481 cgcgcggtaa aatgtgtgcc ccaaacttcg gttggtgcgt cttgtgtggc gaaatcagca
     9541 aaacgaacat atgcgctact gccatctggt gcggtgagat gcaagccatc atgcacaggt
     9601 gaaggagtaa accgtaccat ttgcggaaac tggcgagcag taataaacgt accatcaggt
     9661 tccgtgatca taaagatgcg atcgaaggcc agaccactca catctgccag ggtatgtgta
     9721 agaccaatgc cgcgcatcga tttaacagga tgaataaaaa gccgggttaa tgtcgccacg
     9781 gtgcggtcct caaatgaaaa taagccctca actttatgac atgacgcgct tattagctat
     9841 aatgcgcaac aattttctta gcgcctgtcc cgttaggcta ttttatttgt cagtttggcc
     9901 cagggaagtg ctcgaaatcc tcacgtactt gtgtacgctc cggtttctcc gcgctgtcca
     9961 tgtccagact gactgcaaca attacgccta ctgcggtagg ctcttagagt aaaagtgacg
    10021 atatgaattc tctgtttgcc agtacggccc gtgggctgga agagctgtta aaaactgaac
    10081 tggaaaacct gggggccgtt gaatgccagg tggttcaggg tggggtccat ttcaagggcg
    10141 acacacggct tgtttaccag agcctgatgt ggagccgcct ggcctcgcgt attatgttgc
    10201 cgctgggcga gtgtaaggtt tacagcgatt tagacctcta tctcggtgtt caggcgatca
    10261 actggacaga gatgtttaat cctggcgcga ccttcgctgt ccacttcagt ggtttgaatg
    10321 acaccatccg caacagtcag tacggcgcga tgaaagtgaa agacgcgatc gtcgatgctt
    10381 tcacgcggaa aaatctgccg cgtccaaatg ttgatcgcga tgcgccggat atccgcgtta
    10441 acgtctggct gcataaagaa accgccagta tcgctcttga tctgagtggt gatggtttac
    10501 atctgcgtgg ctatcgcgat cgtgctggta ttgcgccgat caaagaaacc ctggcagccg
    10561 ctattgtgat gcgatccggc tggcagccag gaacaccgct gctcgatccg atgtgtggtt
    10621 ccggtacgtt gctgattgaa gcggcgatgc tggcgaccga tcgcgcacca ggcttgcacc
    10681 gtgggcgttg gggctttagc ggctgggcgc agcatgatga agctatctgg caggaagtga
    10741 aagcggaagc gcaaactcgc gcccgtaaag gcctggctga gtatagctct catttttacg
    10801 gttcggacag cgacgcacgg gtgattcaac gtgcacgcac taacgcccgt cttgcgggga
    10861 ttggtgaact gatcaccttt gaggtgaaag atgtcgcgca actgaccaat ccgctgccga
    10921 aagggccgta cggcacagtg ttgagcaacc cgccatacgg ggaacgtctg gacagcgaac
    10981 cggcgctgat tgcgctgcat agcctgctgg gtcggatcat gaaaaaccag tttggtggct
    11041 ggaatctctc tttgtttagt gcctcgccgg atctgctaag ctgcttgcag ctgcgtgcag
    11101 acaaacagta caaggcgaaa aacggcccgc tggactgcgt acagaaaaat taccacgttg
    11161 ccgaaagcac tccagacagc aaaccggcga tggtagcgga agactacgcc aaccgcctgc
    11221 gtaagaacct caaaaaattc gagaagtggg ctcgccagga aggaattgaa tgttaccgcc
    11281 tgtatgacgc cgatctaccg gaatataacg ttgccgttga ccgttatgcc gactgggtgg
    11341 tggtgcagga gtatgcgccg ccaaaaacta ttgatgctca caaagcgcgt cagcgtctgt
    11401 tcgatattat cgctgcaacc atttcggtac tggggattgc gccaaacaaa ctggtgctga
    11461 aaacccgtga acgccagaag ggcaaaaatc agtaccagaa actgggcgag aagggcgagt
    11521 ttcttgaagt taccgaatat aacgctcact tgttggtgaa cctgacggat tacctcgata
    11581 ccggtctgtt tctcgatcac cgtatcgccc gtcgtatgct cggccagatg agcaaaggca
    11641 aagatttcct caacctgttc tcttataccg gcagcgccac cgtgcatgcg ggattaggcg
    11701 gtgcacgcag caccaccacc gtggatatgt cgcgtactta tctggagtgg gcagaacgca
    11761 acctgcgtct gaatggcctg accgggcgtg cgcatcgcct gattcaggcc gattgcctgg
    11821 cgtggctgcg tgaggcaaat gaacagttcg atctgatctt tatcgatccg ccaaccttct
    11881 ctaactcaaa acgaatggaa gatgcgtttg atgttcagcg cgatcatctg gcgctgatga
    11941 aagatttgaa acgtctgctg cgtgcaggtg ggacgatcat gttctcgaac aacaaacgtg
    12001 gcttccgtat ggatctcggc ggcctggcaa aactgggact gaaagcacaa gaaattacgc
    12061 aaaaaacgct ctcccaggat ttcgcccgta accgccagat ccacaactgc tggctgatta
    12121 ccgcagcctg aaaggaatag taatgtcatt aatcagtatg catggcgcct ggctgtcgtt
    12181 cagcgacgcg ccgcttctcg ataacgcaga actgcatatc gaagataatg aacgtgtttg
    12241 tctggtaggc cgcaacggcg caggcaaatc gacgttaatg aaaatcctca accgtgaaca
    12301 agggctggat gacggtcgca ttatttacga gcaagatttg attgtagctc gtctgcaaca
    12361 ggatccgccg cgtaacgttg agggtagcgt ttatgatttc gttgccgaag gcattgaaga
    12421 acaagcggaa tatctgaaac gctatcacga tatttcgcgc ctggtgatga acgacccgag
    12481 cgagaaaaat ctcaacgaac tggcgaaggt tcaggaacag ctggatcacc acaacttgtg
    12541 gcagctggaa aaccgcatca acgaagtgct ggcgcaactg gggttagatc ctaatgttgc
    12601 gctgtcgtcg ctttccggcg gctggttgcg taaagcggca ttaggacgcg cgctggtgag
    12661 caatccgcgc gtattgctgc tggacgaacc gactaaccat ctggatatcg aaaccatcga
    12721 ctggctggaa gggttcctta aaaccttcaa tgggacgatt attttcatct cccacgaccg
    12781 ttcgtttatc cgcaatatgg cgacgcgcat tgttgatctc gatcgcggca agctggtgac
    12841 ctatccgggg aattacgacc agtacctgca ggaaaaagaa gaagccctgc gcgtggaaga
    12901 actacaaaat gccgagttcg atcgcaaact ggcgcaggaa gaagtgtgga ttcgccaggg
    12961 gatcaaagca cgccgtaccc gtaatgaagg ccgcgtacgc gccctgaaag caatgcgtcg
    13021 cgagcgtggt gaacgtcgcg aagtgatggg taccgcaaag atgcaggtgg aagaggccag
    13081 ccgctccggt aagatcgttt tcgaaatgga agacgtttgc taccaggttg acggtaagca
    13141 actggtgaaa gatttttcgg cccaggttct acgtggcgac aaaattgccc tgattggtcc
    13201 gaatgggtgc ggcaaaacca cgctgctgaa actgatgctc ggtcagcttc aagcggacag
    13261 cgggcgtatt cacgttggca ccaaactgga agtggcttat ttcgatcagc accgcgcgga
    13321 actggatccc gataaaacgg tgatggataa ccttgccgaa ggtaagcaag aggtgatggt
    13381 taacggcaag ccacgccacg tattgggcta tttgcaggac tttctgttcc atccgaaacg
    13441 ggcgatgacg ccggtacgtg cgctttctgg cggtgagcgg aaccgcttgc tgctggcgcg
    13501 tttgttcctc aaaccaagca acttattgat tcttgacgaa ccgaccaacg atcttgatgt
    13561 cgaaacgctg gaactgctgg aagaactgat cgacagctat cagggcacgg tattgctggt
    13621 tagccacgat cgtcagtttg tcgataacac cgttacagaa tgttggatct tcgaaggcgg
    13681 cggtaaaatt ggtcgttatg tcggcggtta tcatgatgcc cgtggtcagc aagagcagta
    13741 tgtggcgctc aaacagcctg cggtgaaaaa aaacgaagaa gccgctgcgg caaaagcaga
    13801 aactgtaaaa cgcagcagta gcaaactaag ctataaattg cagcgcgaac tggagcagct
    13861 accgcaattg ctcgaagatc tggaggcgaa gctggaagcc ctacagacgc aggtggcgga
    13921 tgcttccttc ttcagtcagc cacatgagca gacacaaaaa gtgctcgccg atatggctgc
    13981 tgcagagcag gagctggagc aagcctttga acgctgggag tatcttgaag cgttaaaaaa
    14041 tggtggctga tcgcaaaata gtcgatttaa acagagacag ctgacaacgg taaaattgcc
    14101 tgatgaactg ggtttatcag gcatgaaaac caacactttg ttagtcatct gtaaaatagc
    14161 gcatcattaa ggagtaccaa tgtgcgaaca tcatcatgcc gcgaagcaca tcctgtgctc
    14221 gcagtgtgac atgctggtgg cgttaccgcg ccttgagcat ggtcagaaag cggcatgtcc
    14281 ccggtgtggc acaacgttaa ccgtggcgtg ggatgcccct cggcagcgtc ccaccgccta
    14341 tgcgttggct gcactgttca tgctgttgct gtccaacttg tttccttttg tgaatatgaa
    14401 cgttgcagga gttaccagtg aaattacatt actggaaatt cccggcgtgc ttttttctga
    14461 ggactacgcc agcctcggca cctttttcct gttgtttgtg caactggttc ccgcgttttg
    14521 tctgataacc attctgttac tggtgaatcg cgcggaatta ccggtccgtt taaaagagca
    14581 actggcacgg gtgctttttc aactcaaaac ctggggaatg gcggagattt tcctcgctgg
    14641 tgtgctggtc agtttcgtta aactgatggc ttacggcagc attggggtag gcagcagctt
    14701 tctcccctgg tgtttatttt gtgtcctgca actgcgcgct tttcagtgcg ttgatcgtcg
    14761 ctggttatgg gacgacatcg ccccgatgcc agaactgcgc cagccgctaa aaccaggcgt
    14821 cacggggata cgtcaggggc tgcgttcgtg ctcctgttgt acggcaatcc ttcctgctga
    14881 tgaacccgtg tgcccgcgtt gtagtaccaa agggtacgtt cggcgtagaa acagcctgca
    14941 gtggacactc gcgctgcttg taacgtccat catgctgtat cttccggcta atattttgcc
    15001 catcatggtg acggatttat taggctcgaa gatgccgtcg acgattctcg ctggggtcat
    15061 tctgttatgg agcgaaggct cttatcccgt cgctgcggtg atctttctgg ccagtattat
    15121 ggtgccaacg ttaaagatga tcgccatcgc gtggctgtgt tgggatgcca aagggcatgg
    15181 caagcgcgac agtgaaagaa tgcatttgat ttatgaagtt gttgagtttg taggccgctg
    15241 gtcgatgatt gacgttttcg ttatcgcggt gctctcggcg ctggtgcgta tgggaggttt
    15301 aatgagtatt tatccggcaa tgggtgcatt aatgtttgct ttagtcgtca taatgacaat
    15361 gttttctgct atgacgtttg acccgcgttt gtcgtgggat cgtcaacctg aatcagagca
    15421 tgaggagtcc tgacagtatg gaatctaata atggggaagc caaaatccag aaagtgaaga
    15481 actggtctcc cgtgtggata tttcctatcg tcacggcgct cattggggcc tgggttcttt
    15541 tttatcatta cagccatcag ggaccggaag tgaccctgat caccgcgaat gcggaaggaa
    15601 ttgaaggtgg caaaaccacc attaaaagcc gtagcgttga cgtcggcgtg gttgaaagcg
    15661 ccacactggc tgatgatttg acgcacgttg aaatcaaagc gcggctgaat tccggtatgg
    15721 aaaaattgct gcataaagac accgtctttt gggtggtgaa accgcagatt ggtcgagaag
    15781 ggattagcgg tctgggaacc ctgctgtctg gggtttatat cgaactgcag ccaggcgcga
    15841 aaggcagcaa aatggataaa tacgatttgc tggactcgcc accgttggcc ccgcctgatg
    15901 cgaaaggtat ccgtgtgatt ctcgatagca aaaaagccgg gcagctctcg ccaggagatc
    15961 cggtgctgtt ccgtggctat cgggtaggtt cggttgaaac cagcaccttc gatacacaaa
    16021 aacgcaatat cagctatcaa ctgttcatca atgcacctta tgaccgactg gtgaccaaca
    16081 atgttcgctt ctggaaagat agtggcattg cggttgatct gacgtcagca gggatgcgtg
    16141 tggagatggg ctcattgaca acgctgctga gtggcggtgt cagctttgat gtgccggaag
    16201 gtctggattt agggcagcca gtggcaccga aaacagcttt cgttttgtat gatgatcaga
    16261 agagcattca ggattcgttg tacaccgatc acattgatta tctgatgttc tttaaagatt
    16321 cggtacgcgg tctgcaaccg ggagctccgg tagagttccg gggtattcgc ctgggtaccg
    16381 taagcaaagt gccattcttt gcgccgaata tgcgtcagac atttaacgat gattaccgta
    16441 ttccggtact gattcgtatc gagccagagc ggctgaaaat gcagcttggc gaaaatgcgg
    16501 atgttgttga gcaccttggc gaattgttga aacgtggttt acgcggatcg ctgaaaaccg
    16561 gaaacctggt cactggtgca ctgtatgttg atctcgattt ctatccaaat acgcctgcaa
    16621 taaccggtat tcgtgaattt aatggttatc agattatccc gaccgttagc ggcggcctgg
    16681 cgcaaatcca gcaacgactg atggaagcgt tggataagat caacaaactg ccattgaatc
    16741 cgatgattga acaggcaacc agtacgcttt ctgaaagtca gcgcacaatg aaaaacctgc
    16801 aaacgacgct ggatagcatg aacaagatcc tcgctagcca gtcgatgcag cagttgccga
    16861 cggatatgca gtcaacgttg cgtgaattga atcgcagcat gcagggcttc cagcctggct
    16921 ccgcagccta caacaagatg gtggcggata tgcagcgcct tgatcaggtg ttgcgagaac
    16981 tgcaaccggt gctgaaaacg ctcaatgaga agagtaacgc gctggtattt gaagcgaagg
    17041 acaaaaaaga tccagagccg aagagggcga aacaatgaaa aagtggctag tgacgattgc
    17101 agcactgtgg ctggccggat gcagctccgg cgaaattaat aaaaactatt accagttacc
    17161 tgtggtgcag agcggtacac aaagtaccgc cagccagggc aatcgtctgt tatgggtaga
    17221 gcaggtcact gttcctgact atctggcggg gaatggtgtg gtttatcaaa ccagtgatgt
    17281 gaagtatgtg attgccaata acaacttgtg ggccagcccg ttggatcaac agttgcgcaa
    17341 caccctggtt gccaacctga gtacgcaact gcccggctgg gtggttgcct cccagcctct
    17401 gggaagcgcc caggacacgc tcaatgttac cgtcacggag tttaacggtc gctatgatgg
    17461 caaggtcatt gtcagtggtg agtggctgtt gaaccaccag ggacaactga tcaaacgtcc
    17521 gttccgtctg gaaggggtgc aaactcagga tggttacgat gagatggtta aagtactggc
    17581 cagtgcctgg agtcaggaag ccgcttctat tgcgcaagag ataaagcgtc taccttaatt
    17641 ataaagattt gtaaatataa ccgcctccgg tatgttgcct gaggcggttt ttttgtctct
    17701 aacgtgcgga aaaagttgtt cctcttcaca ttttttgtac aaccggcata cccgtgtagc
    17761 tcacaaatat gacagtggcg tgaattttgc gcattgacgg cagttatgat tcgcggtatt
    17821 gcttaactgt gattgcacat ttagtaatca ctgttttctt ttccaccaga aaccagtatg
    17881 agggaaacga ggcatgaaga gacaaaaacg agatcgcctg gaacgggcac atcaacgtgg
    17941 ttatcaggcc ggcatcgccg gacgctcaaa agaaatgtgt ccctatcaga cgctgaatca
    18001 aaggtcacaa tggctgggag gctggcgaga agccatggcg gacagggtag taatggcctg
    18061 attctgtctc tttaaaaaga aacctccgca ttgcggaggt ttcgcctttt gatactctgt
    18121 ctgattataa tcagaaggca gacgtatcct ggaacagacc gactttcagg tcgctggcgg
    18181 tatagatcag acgaccatca accagcactt cgccatccgc caggcccata atcagacgac
    18241 ggttaacaat gcgtttaaag tgaatacggt aggtcacttt tttcgctgtc ggcagtacct
    18301 gaccagtgaa tttcacttcg ccaacgccca gcgcgcggcc tttaccttcg ccgcccagcc
    18361 agccgaggta gaaccctacc agctgccaca ttgcgtccag gcccaggcat cccggcataa
    18421 ccggatcgcc aataaagtgg catccgaaga accacagatc cggattgata tccagttctg
    18481 cttcaacata ccctttgtcg aagttaccac ccgtttcggt cattttgacc acacggtcca
    18541 tcatcagcat gttcggtgct ggcaattgcg ggcctttagc gccaaacagt tcaccgcgac
    18601 cagaggcaag aaggtcttct tttgtatagg attcgcgttt atctaccatg ttctctgtaa
    18661 gccttatttt attgaagcac gcaggatagc taacacgtgt acgctgaaca agtccgatca
    18721 gttcggaata aaccagttca gccaacgtaa tggccatgga aaacggtgac gtccttcctg
    18781 ttgcgatgct tgcgcgatac gttcctggat ggtttgcatc agcgtcgttt ggccttcgcc
    18841 atcccacacc agatttaata ataacggcag tgcgtcagtc acatcgtcta ctgcccagat
    18901 ggtgaatttg ccttcttcta ccgctttcac cagttcactg tgaagactta aatggcgaac
    18961 gttagctgtg gggataatga caccttgttt cccggttaac tcacgttgct ggcaaatagc
    19021 aaagaagcct tcgattttct catttaaacc accgaccggc tgggcgcgac cgaactgatc
    19081 gactgaacct gtgatagcga tactctgatt caccggcaca tcggcgaggg cgcttatcag
    19141 ggcgcagagt tcagccatcg aggcactatc tccatcaact tcactgtatg actgctcaaa
    19201 tgtcagcgat gctgagaagg ggatctgttg ctcaagctgt agttccgaca tcaggaacgc
    19261 ttgcatgatc atcatccctt tcgcatggat attgccgcca agctccgctt tgcgttcgat
    19321 gtcggtgaat tcaccatcgc caatatgcac aacgcagcta atgcgagaag gttcgccaaa
    19381 agcgcgtgga tgacccggaa attcaatgac cgaaagggcg ttaatttgcc cgatgcgttc
    19441 gccttcggtt tcaatcagga tttgctcctg aaggatctca tcctgcatac gttcagcgag
    19501 gaaaccttcg cgccattcac gctgctgcag cattaagttt agctgctcgc cggagaaggt
    19561 gtcgccatca cacagggagg cgacctcttt acactggcgg aggatccact gcgggctaag
    19621 cggaagtgtt tcttgttcac cggtgtagcg tgctgcttcg cggataagta ccggccaggc
    19681 atccgctccc ggtgcaggta agtgattatg tctggcggta aatgtcaccc agcgacacca
    19741 ctgggttact gactctgcat cgacaatctg cagagtatct tcaaattcgc tataaatagc
    19801 ctgctctgaa agctctggct ccatctcctg gaaatcagac aatgattcgc gttcgcctac
    19861 cagaatgact ttcagcttca atggcatcga aggcacagag acggggagag ggcgcgactc
    19921 atcaaacgca acccagtcaa aacgctcgcg gttaacgata tttttcagcc gcatccacag
    19981 cagaggttgc gccagcagtg tacgcaaaga gatgatgaga ataccgccat ttgcctgatg
    20041 caccagacca ggctgcaggg taatgtcgcc attaaactgg cgcaggcagc caaagagttg
    20101 ctccgcttct acccagtcgg cagcgacaac ttgcgttaaa gtcgcaaaat tatcatctgc
    20161 actcactgcg tgacgtaagc ggatggagtg gccggaaacc tcatagtgac cgcccaccag
    20221 ttggcctgca tcgctttgta gcgtacgcgc ggcattggca ataagattca gatactcaga
    20281 ctcttccggg gccttcgcca gcataaaaga ggaggatgct cgcgtatgca gcaactgctc
    20341 cagtgcgaat tgcagtcgcg gttgagtatc actgaataaa ggatcgtttt cgtcaatcaa
    20401 atgtggctga gcaaatattt cctgatagct atcggtatca ggaaccaggt cacgccatgc
    20461 aagtttcgta atggtcaaag ttgatgtttt ttagtctgtt gtcaaagccg cgattatacc
    20521 gtaaccggca ctacagcaca cgtagaaagc accgacaata ctcctggcat gggcgttaaa
    20581 gctcacagga tggagattct tttcttcact ggcctaaaaa gctgatattc tgtaaagagt
    20641 tacacggtaa cattgagatc gctatgaaat atcaacaact tgaaaatctt gaaagcggtt
    20701 ggaaatggaa gtatctggtg aagaagcatc gcgaagggga gttaatcacc cgttacatag
    20761 aagccagtgc cgcccaggaa gccgtcgatg tgttgctctc gctcgaaaat gaacccgtgc
    20821 tggtaaatgg ctggattgac aagcatatga atccggaact ggtcaatcgc atgaagcaaa
    20881 ccattcgggc aagacgaaag cgccatttta atgcggaaca ccagcatacg cgcaaaaaat
    20941 cgatcgatct ggaatttatt gtctggcaac gtctggctgg tcttgcgcag cgtcgcggta
    21001 aaacgctttc tgaaacgatt gttcagctga ttgaagatgc ggaaaacaaa gagaaatacg
    21061 cgaataaaat gtcttctctg aagcaggatc tgcaggcatt gctgggtaag gaataactga
    21121 cgaaagtcag ttcaatttac taaaggcaaa aaaaccccgc agcagcgggg tttttctacc
    21181 agacgagaac ttaagcttgc ggctgagtta caacgtcttt gataccttta acttcgatct
    21241 ctacgcgacg atccggagcc aggcagtcga tcagtgcagc acgctgtttc acgttgtcac
    21301 aggtgttgcc agtaaccggg ttggattcgc ccataccacg tgcggagatc ttgtctgccg
    21361 ggataccttt ggagatcagg taatcaacaa cagactgagc acggcgctcg gacagaccct
    21421 ggttgtaagc gtcagaaccg atgcggtcgg tgtaacccag aacaactacg gaaccgtctt
    21481 tcggatccag gttgctcagc tggctgtaca gctgatccag agcagcctga ccttccggtt
    21541 tcagggttgc tttgttgaag ttgaacagaa cgtcagactt cagagtgaag tgcttggtct
    21601 gtacttccgg tgccggagct ggagccggag caactactgg agctgcttcg ccctgaccga
    21661 aacggtagga aacacccagg ctcagcatgc cgttgtccgg acgagtgccg atggtgtgtg
    21721 cgtcaccgat gttgttggtc cactggtatt ccagacgggt agcgatttca ggagtgatcg
    21781 cgtactcaac accgccagcg aagaccggag aaacgccggt gtcgtggttt ttaccataaa
    21841 cgttggattt agtgtctgca cgccatacca taccacccag acgagtgtag atgtccaggt
    21901 cgtcagtgat tgggtaaccc agtttagcgg tcagttgaac gccctgagct ttgtatgcac
    21961 cgttttcaac gctgcctttg tacggcatac gacctaacca gtcgtaaccc atttcaaagc
    22021 caacatacgg gttaacctgg taaccaccaa aagcaccagc gcccagttgg ttttcatggg
    22081 tcgggccatt gttgttgatg aaaccagtgt catggtactg ggaccagccc agtttagcac
    22141 cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa ccagccagtg
    22201 ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca tccaaaatac
    22261 gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga tctgctcaat
    22321 attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta aagtctacaa
    22381 cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa gtcttgtata
    22441 aggtatgttt aatctttttt gtcagcgaca atttacagaa gaaaatcgcg gaaaccgctt
    22501 cagacaagcc tccgcaagga aaattagtca cgactgaaag cattggctgg gcgacaaaaa
    22561 aagttccagg attaatccta aatttactta atgatacaaa ttagagtgaa tttttagccc
    22621 ggaaagttgt ctcgtggcgt gagaggatgc gcttaccgga cgcataataa accccatagc
    22681 gttaccttca tttgccgcat caacaagttc agcatgctct tcttcagtca aatcatctgc
    22741 caaccaaccg atcaccacac tgtaattgcc cgtgcgtaaa gcgcgaacca ttgactccac
    22801 agtgtggcaa ggggagagct ggctaatctg cattactttc gttaagggta gcccagatgc
    22861 ctgaacccat tcccgactca gtttttgttg cggtgttaac cagagttgcc agcgcgattg
    22921 ctgaccgagt tgctgtaaca atggcaacag tagaagttgc gtcatcatgg gctgatcttc
    22981 gcgatagaca acttcactga taagcccggc tgtagtgttt tccgtagaga cacgcgcaat
    23041 tttacttgct gcggatgaga acgacgaaga acgatgtgca tagcctgaag tgtacataat
    23101 caatccagcc cctgtgagtt actgtatgga tgtacagtac atccagtgac aacaaagatc
    23161 aaccctattt tcggaaagag cctcgcaaat tttgtcgttg gtgacgggaa aacataaatt
    23221 aatcttgccc cttaagaata agttgcctat tttcgtagtt aacggatccg ttaatgtgaa
    23281 tcattctttt atgttatgat tttaaaagga attttatgaa aagcctctcc tataagcgga
    23341 tctataaatc acaagaatac ctggcaacgt tgggcacaat tgaataccga tcattgtttg
    23401 gcagttacag cctgaccgtt gacgacacgg tgtttgcgat ggtttctgat ggtgagttgt
    23461 atcttcgggc ttgtgagcaa agtgcacagt actgtgtaaa acatccgcct gtctggctga
    23521 catataaaaa gtgtggccga tccgttaccc tcaattacta tcgggttgat gaaagtctat
    23581 ggcgaaatca actgaagctg gtgcgtctgt cgaaatattc tctcgatgca gcgctgaaag
    23641 agaaaagcac gcgcaatacc cgggaaagac tgaaagattt gcccaatatg tcttttcatc
    23701 tggaagcgat tctcggggag gtggggatta aggatgtacg ggcgttacgt atacttgggg
    23761 caaaaatgtg ttggttgcga ctgcggcagc aaaacagtct ggtgacagaa aagattctgt
    23821 ttatgcttga aggtgccatt atcggcattc atgaagctgc gctcccggtg gcacgccgcc
    23881 aggagcttgc agaatgggct gactctctta cgccgaaaca ggagtttcct gcggaacttg
    23941 agtaatctgg cgttgcagac gaccaatctc aggcagtaat gcaatcaata atccaacttg
    24001 ttgtacgacc agaggttctt tgctgtctgc ccgtggttca agttgctgca tccgctgttt
    24061 caggctagct aatgcctcat tgacgcgttc ttcatcagca ggttgatgat gtaacgcgtc
    24121 atcaacatag caaactgcgt catcaagaaa cgccagaatt tcaggattag ttaactgctc
    24181 ccggtgagca ccgagggctg agatatagct ggtaaacgta tggttaaggc acagcaaccg
    24241 aaacgcggct tcgcgaattt gcggggtaac gttcggctcg ctggacatat ttgataccac
    24301 cgacgccagc tcagcatcac ggttgtgtgc atcgcggcgg gcaatacgat acgccagacg
    24361 gttatcacgc ccctgatggt attgctccag tatggcatcg agataccgac agttggcctc
    24421 tgtggcgcgt tcgagcatgc gcggcagatt gcgaaactgc cagtcaggcc agatgtagct
    24481 cactgccgcc cacgcaatgg cacaaccaat cagcgtatcg attacgcgag gtaacgctac
    24541 ttcaaaacct tcacccagta agttaaaaca cagtagcacc aaaagtgtga tgaacatcgt
    24601 tgcatgagcg tattgcacgt tacggaaggc aaaaaagagc acgccggtaa taaccagcag
    24661 caccagctgc ccttccagtg atggcacaaa ccacagcaca ggaatgccaa tggcgatacc
    24721 taccagcgta ccaataatcc ttaacttcag gcggtggcgc gtggcgttat agtttggctg
    24781 gcagacaaac aaacttgtca gcaagatcca atacccgtga tgcattccgg ttatttgaat
    24841 gatggcgtag ccgaagcaca acaccagcga cattcttacc gcatgacgga agagggcgga
    24901 ttccggcgtg aagtgacggc taagacgcag ccagatatca ctcaacccgt gcgggctgtc
    24961 atcagcgagc tcattttcgt cattattatg gggtagtgcc tgggcctgtt ctgattcaat
    25021 tgttgccagt tgggcatcaa tggcgcgtaa attgttcagc aaaaatccca gtgttttgag
    25081 taaatcggcg ggtgcgccgt tatcgcgcat ccgctccagc gcagcatcaa tatgcgtaaa
    25141 agcgcgctca aaatgcggat catgttgata aggctgacgc aacaaaatac agcgtgacag
    25201 ttgctggcac gcctggccct gcatcgacat cagccgctga aaacggaaca gcacgtcgct
    25261 gtggcgaaaa tgttcacgca atgtttgata ctgaatatga gaagagctgg cacgctcgtg
    25321 aatatcctgt gcgacaaagt aataatgcag cgtgcgacgc gttccccgtt gaccacgatc
    25381 gccacgtaag cgggtcagca gcgagagttt cgtctgattc aatgtcgcca tcagcagacc
    25441 gttggcgaga gccaaatcgt acagcggtgc ctggctttca tcttcaatat caggatcaaa
    25501 catgcgcgac ttgagctcaa gataacgcgc cagttgttca tagcaacgcg ccaggttgtc
    25561 ctgcagcggg cggaccggga acagcagatg accaataagt gtcaggacgt tgtaccagac
    25621 ggcaccggcc agcagataca tcggctgctg ataccagtgc tcatacagtg atgttcccaa
    25681 catagtgtaa atggcgatca gcaatgcacc gaaggcaatt gttgcatagc gttgacccag
    25741 accgccgagc aaaatgaagc cgctggtaga gagcgttaag ccaatcgcaa atagccaggg
    25801 ccagggaaac agcaattcta ctgaggccga ggcgataaaa aagcagaaca gcgtaatgat
    25861 gaggttacgc aaacgtcccg ccagtcggtc atcgagatcg gtcagcgccg ctgccaccat
    25921 ccccagcgtt agcggaatcg tcagttttac atcacccagc caccacggaa acgctgtggt
    25981 tccacaaagc gcaataaaaa tacgcgcgta atacagccag gcgctgttcc aggtatagcg
    26041 tttgagcaaa ggacttagca taaaggccat attagtggat tattcaaaac gacgacgtgc
    26101 attggcttca cgcgcagctt gcgctgtttc taccgatacc acgcgacgac caaccggcca
    26161 tagtgcaata gcggcaattt taaagttcgc aatgccgaca ggaatgccaa tgattgaaat
    26221 acattgtgcg atgcccgttg caatgtgcat caggcataac caccagccaa agaaaatcag
    26281 ccagaaaata ttcaataccg taccgccagt attcagcagt acatttttgc cagccgggtt
    26341 cagttcatcg acatgaatag cttcattgcc ataaggcacc agagacagtt tagtgatctc
    26401 ccagcaggat cgtgtcagcg gtaaggtaaa aatcagcaca atactgacca gagtcgccaa
    26461 cagccagccc agagtggtgg caaatccgcc aagcacaaag ttcagaatgt tcaaaacggt
    26521 acgcataaaa cctcgcttta ctgtggtttt cagtaatggg cggcaattgt aacgtttttt
    26581 tgggctggag cacgttttct ctgacggtta cactgataag aaataatttc gtgtggatct
    26641 acagagtcat ggaactgaaa gcgacaacgc ttggaaaacg tctggcacag cacccttacg
    26701 atcgggcggt gatcctcaat gccgggatta aagtctccgg cgatcgccac gaatacctta
    26761 ttcctttcaa tcaattactg gcgattcact gtaagcgcgg tctggtatgg ggcgagctgg
    26821 aatttgtact gccggacgaa aaagtggtgc gtctgcacgg caccgaatgg ggcgagacgc
    26881 agcgttttta ccatcatctt gatgctcact ggcggcggtg gagtggcgag atgagcgaaa
    26941 ttgcgtctgg tgttttacgc cagcaactgg atttgattgc cacgcgcact ggggaaaata
    27001 aatggctgac gcgtgagcaa acctctggcg tgcagcaaca aatccgccag gctttgtcgg
    27061 cgttgccgtt gccggttaac cgactggaag aattcgataa ctgccgtgag gcgtggcgta
    27121 aatgtcaggc ctggttgaaa gatattgaaa gcgctcggtt gcagcataac caggcgtata
    27181 ccgaagccat gcttaccgag tatgcggatt ttttccgcca ggtcgagtct tcaccgctga
    27241 atccggcgca ggcccgggca gtcgttaatg gcgagcattc tctgttagtg ctggcaggtg
    27301 caggaagcgg aaaaacgtcg gtgctggtgg cccgtgcagg ctggttgctg gcgcgtggtg
    27361 aagcgtcccc tgagcaaatt ttattgctgg cgtttggtcg caaagccgct gaagagatgg
    27421 acgagcggat tcgcgaacgg ctacataccg aagacattac cgcacgcacg tttcatgcgc
    27481 tggcgctgca tattattcag cagggcagca aaaaagttcc gatagtcagc aaactggaaa
    27541 atgataccgc tgcccgtcat gaactcttta ttgctgagtg gcgcaagcaa tgcagcgaaa
    27601 agaaagcgca ggcgaagggc tggcggcaat ggctgacgga agaaatgcag tggtcagtgc
    27661 cagaaggtaa cttctgggat gatgaaaaat tacagcgtcg ccttgcctct cgcctcgatc
    27721 gctgggtaag tctgatgcgg atgcacggtg gtgcacaggc agaaatgatt gccagtgcac
    27781 ccgaagagat tcgcgatctg ttcagtaaac gtatcaagtt gatggccccg ttattaaaag
    27841 cctggaaagg tgcgctgaag gcagaaaacg ctgtcgattt ttcgggcctt attcatcagg
    27901 cgattgtgat tctggagaaa ggtcgcttta tcagcccgtg gaagcatatt ctggttgatg
    27961 aatttcagga tatctcgccg cagcgggcag cgttgttagc ggcattacgc aagcaaaaca
    28021 gtcagacgac gttgttcgcc gttggtgatg actggcaggc gatttaccga ttcagcggcg
    28081 cgcaaatgtc gctcaccacc gctttccatg aaaactttgg tgaaggcgat cgctgtgatt
    28141 tagacacgac ttaccgtttt aacagtcgta tcggtgaggt ggcaaaccgg tttattcagc
    28201 agaacccagg ccagttgaaa aagccgctaa acagcttaac caatggagac gaaaaagccg
    28261 tcacgttatt ggatgagagt caacttgacg ctttgctgga taagctctct ggttatgcca
    28321 aaccggaaga gcgcattctt atcctggcgc gttaccatca catgaggcct gccagcctgg
    28381 aaaaagcggc aacacgctgg ccgaagttgc aaatcgactt tatgaccatt catgccagca
    28441 aagggcaaca ggcggattac gtcatcatcg ttggcttgca ggaggggagt gatggttttc
    28501 cggctgcggc gcgggagtcg attatggaag aggcgctact gccaccggtt gaggatttcc
    28561 cggacgctga agaacggcgg ttaatgtacg tggcgctgac ccgggcacgc catcgggtat
    28621 gggcactgtt taacaaagag aatccctctc cctttgtgga aatactgaaa aatctggatg
    28681 tgccggtggc gagaaaaccg taagaaacag gtggcgtttg ccacctgtgc aatattactt
    28741 cagacggtcc gcgagataac gctgataatc ggggatcaga atatcgaccg cgtcgttgaa
    28801 atgcggcgac tggataatga agtctgccgt tgccacgttg gtggcgaccg gaatgttcca
    28861 taccgtcgcc agacgcagca aggctttcac gtcaggatcg tgcggcacgg catttagtgg
    28921 atcccagaag aaaatcaata catcaatttt cccttctgag atcaatgcgc caacctgctg
    28981 gtcacccccc attgggccac tcaacatcgc gttgacgttc atgccggttg cacgggaaat
    29041 caagttaccg gtagtgcctg ttgcatacag tacgtgttgt tccagtaacg gttgatgccg
    29101 ttccacccag ctcatcagca tttgtttgca gtgatcgtgt gccaccagcg caatatgttt
    29161 ccgcgcaggt aaagtgcgag tcgtcagttc cataatgtac atccgtagtt aactttccta
    29221 cagattactg taagcactta tcgctgcaag ataaagaccg aaaaagcctg cgcacaggca
    29281 caaaaatctc aggaagatgg ttgtttttcc gcccactgca ggaaagtatt tcgcgtttgt
    29341 gggtcagcca gtttaaacca atacttcagc cgttgttctg tgagcacctg agactgcggt
    29401 ggaatagcat ccagctcgga aacacccgaa agcagcgtac tgtcatctgc catcatcgtg
    29461 gcaaattgcg gtagtgaagc gcgtttcgcg gatttgttat agcgttcaac ctcgacctca
    29521 taatcaatcg tttttgccgt tgaggtaatg gcgagaatat ccagctttac cggaatcggc
    29581 gtcgcatcgc catccaacaa ttcaaggcgc ggcgcggcat caaaatggtt agcttcccgc
    29641 tcattctcca ggcgaggcag gcgaaaattg acctggttga tgagctgggt attaaaactg
    29701 accaccagcg gtggggagat atacagccgt tcttcactat tggaaagatg aatcgtcttc
    29761 tcaacgcgaa acactaactg atgcggtcca ttatccagtt caatgctgtc cgcaccgcgc
    29821 agcagagagc tggaaacctt cttgccgtcg agtaccagaa gatcgacatc ggttgaaagc
    29881 cgcagcgtgg tggcaaaaac ggataccggc aaacataatg caatcaaggt ggtcacgatg
    29941 ccggttttca taggtagctc ctgtcaaaag accgcccgaa taataatgtg tcaaaatttt
    30001 tctttaatca tacttactaa catatagaca tatttcccgc ttttgctctc attcattcgt
    30061 attagctgca tggttggcat gtcggctttg tcgtacactt tgttaaacag tctggaggaa
    30121 ataatgaaag aaaccgatat tgcaggcatt ttaacgtcca ctcacaccat tgcgctggtg
    30181 ggggcaagcg acaaacccga tcgtcccagc tatcgcgtga tgaaatatct actcgaccag
    30241 gggtatcacg tcattccggt ttcgccaaaa gttgccggca aaacgctgct gggccagcag
    30301 gggtatggca cgctggcaga cgttccggaa aaggtcgata tggtcgatgt tttccgtaat
    30361 tcagaagcgg cgtggggcgt ggcacaggaa gctattgcca ttggcgcgaa aaccctctgg
    30421 atgcaactgg gggtgattaa tgagcaagcg gcagtactgg cacgggatgc cgggttgaat
    30481 gtggtgatgg atcgctgccc ggctattgag atccctcgcc tgggcctggc caaataaaaa
    30541 atccccggaa ggcaaaaacc ttccggggat tcgttcaggg atcagttacg cagacgcggg
    30601 gcctggagtt gtttgcggat ggtctgcgcc agctcatcca tggatggctg ttcaggatgt
    30661 tcatcctgca attcgctact tagctgggct tcagccaggt aggtatgaac cggtaggccg
    30721 ttatcgtcct ccatcaccac gtgataccac ggagcggcgc gaagctcgtc attcaccgcc
    30781 agctcatcag gcgacggttc agagagcgaa taaaccgggt cgatatccac gaccactccg
    30841 agataaccta acagggaatg gcggacctgc tggccgatac cgaatttgct ggcaatcata
    30901 gtcacctccc gggaaatctt tacctttgat ataagggtaa aattccacat ttcaagttac
    30961 atgacgcgac aggcaaaccc tttcagatat agcccttccg gataggtagc gatcaccgga
    31021 tgatcggctg cctgacggaa ctgctctata aattgtacat cacggccggc atcaattgcg
    31081 gcatccgcga tgattttctg aaataaatcg ctggtcatca gaccggaaca ggagaaagtc
    31141 aggagaatac cgccttcatt cagcaactga atcgccagca tgttgatatc tttatagcca
    31201 cgacacgcgc ccatcaactg gcttttattc tcaacaaact tcggcgggtc catcacgata
    31261 acgtcaaata tttcaccgcg atcgcgataa gtacgcagca atttaaagac atcatcacgg
    31321 acaaactcag ccttgctcag atccagtttg ttcagctcaa cgttctgccg tgcaatatcc
    31381 agcgcttcct gggaggtatc aacgctgaca acctggctgc aaccgcccat cagtgccgat
    31441 acggcgaaac caccggtata ggagaaacag ttcagcacac gtttattttc aacgtagcgg
    31501 cgggtagcca ggcggctatc acgctggtcc aggtagtagc ccgttttgtg tccgtgctga
    31561 atatccacca gcagcttcat gccgtgttct tcaatcggca gcagggcagg cggcaactcg
    31621 ccggtgacga ggccctgggt cagctccatc ccttcttttt tacgtaccgc aacatcgctg
    31681 cgatcgtaaa tcgcacattc cgggtacagc gtttgcaggg cactaactaa tgccgcgcgc
    31741 tgatattctg ccccagcact cagcagttgc agcaccagaa aattaccgaa acgatcgata
    31801 gtaatacccg gcaggccatc agattctccg gcgattaaac gatagctgtc gaggccatct
    31861 ttttgcgcca gccagtcacg ccatttttgt gcttgttgca aacggcggga aaaaaaagca
    31921 atgtcgatag actcagacgg gtcaaacgtc cagacgcgcg cccggatttg cgaagctggt
    31981 gaataagcgc cgcgtgctaa ccattttccc tgatgatcaa caatatcgat ggtttcaccg
    32041 aggctggctt taccttccat gcgggcaacg gccccggaaa agacccacgg atggcgacga
    32101 agtaatgatt tttcgcgccc tttggctaac actaaacgta cactcataat ttacttttct
    32161 gtcgatgcaa agaaatgggc gtcattgtcc ggagttttcc atggatttgc aacgcgtcca
    32221 ttaaggataa ggagagcagc aatgtcgaaa gtctgcataa ttgcctgggt ttacgggcga
    32281 gttcagggcg taggatttcg ctacaccaca cagtacgaag cgaaaagact gggtttaacc
    32341 gggtacgcca aaaatcttga cgatggcagc gtggaagtgg ttgcctgcgg tgaagaaggg
    32401 caggtggaaa aattaatgct gtggttaaag agtggcggcc cgcgttctgc gcgtgtagaa
    32461 cgggtgctca gcgagccgca tcatccctcg ggggaattaa ccgattttcg tatccgttaa
    32521 atacatttta ccggttttgg caggccagca attttggtgg cttgcttagc cggacctttc
    32581 ggaaacagtc ggtataaata gcggctgtta cctttttctt cgccaaattt attcgccatc
    32641 gcttttacca gcatacgaat cgccggagaa gtattgaatt ccagatagaa atcacgcaca
    32701 aaacgcacca cttcccagtg ttccggtgac agcgaaatcc cttcgttctc tgcaatcacc
    32761 actgccagcg gctcactcca ctggctgctt tctttgagat agccttcggt atccgtttct
    32821 atctctttac cttcgaagat cagcataatt attactacct taatcagact ggcggcagtg
    32881 taacaaaaaa caaagccccg cataaagcga ggctatgaaa gtgttagcgg gtgagattaa
    32941 tcgcggctgg cgaagcccag aatgctcagc aggctgacga agatgttgta cagcgaaaca
    33001 tacaggctaa ccgtggcacg aatatagttc gtttcaccgc catgaacgat gttgctggtt
    33061 tcaaacaaaa tagcgccaga ggagatcaaa atgaagaccg cgctgatcgc cagatgcaga
    33121 gcaggcagct gcaggaagat attagcaacc ataccaatca gcaccaccac aatacccgcc
    33181 atcagcatac cgccgaggaa cgacatatct ttgcgggtgg tcagcacata tgcagagcag
    33241 cagaagaaca ctaacgccgt tccgcccagt gccatagcga ttacgtcacc cattccggca
    33301 gacagatagg tgttcagaat aggcccgagg atataaccca gaaaaccggt aaaggcgaat
    33361 gcggagataa tcccggtcgg cttattcgcc gttttatagg tcaggaacat caaaccatac
    33421 atacccacca gcgtcagaat cagacccgga gatggcagca tcagcaccgt actggcagtt
    33481 gcggtaattg ccgaaaaggc cagcgtcagg ctcagcagaa aataggtatt acgcagcacc
    33541 ttatgggtgc taagcagtga tgtacggtca tgtgaagaac taacaatacg atccattgag
    33601 tcactctcta tgacagatgt aattaattaa gcagcataat gataatgcgt aagggcaccc
    33661 agaagtttta cccatcttta cgcatttgat ctggaacagg tttaacagcg gattatcagg
    33721 tcattaagca aatataacgc cctgagaatt tcgacaggca aaagaaaaag gggttagcat
    33781 ttagctaacc ccttatctta tttggcggaa gcgcagagat tcgaactctg gaaccctttc
    33841 gggtcgccgg ttttcaagac cgaagaaaaa caaataaaaa tcagtgcaat acgtgaaatt
    33901 gctgaaaagt taatcaaatt taacaccaag aaaaatcaag gtattaggaa gttgctcata
    33961 gctagtatac caagtctttt tgccagaaat ttttctggat tgtctacttg ctaaccacga
    34021 gcggcaaatc cagtgttggt gtgatttttg ttttacgatc gtaaatcaac acctgatttt
    34081 ctgttttatg cccgctgaaa atttgtttgt cgcgactgct gccttcgtaa tctgagatcc
    34141 ccttggcttt tatgtcgtga aaattgcacc cgaacggaac gccaactttt tgctcggctg
    34201 cgcgtttagc ctgattccac cagttattca gcgttttagc tatgactttc ccgcctttgg
    34261 ttgtattgat cacatattcg catgtacagg aagatacatt tcgggctaac tggatcgctg
    34321 tacgtaatcg cggtgaccat tccttgattt gtttggtgcc ggttttgttt tgctcaatgt
    34381 agatcccttt atccataata tcctgccatt tcaactcaag cacatcaccg agtcttgccg
    34441 cacagagata ggaaatctcc attgcaatgc gtaactgtgg aattgcttcc gcatatatcg
    34501 ccgcatactg ttcatcggtg atgtaaacag tgcgggcttt aagagagaat tttctgactc
    34561 ctttgcatgg gttattcttc acatacccac gctcatatcc ccacccgtat actcgactca
    34621 ggcttgccag ttcatgattt gcctgggtct tgctctcaag ccctcgttta tccatgaaaa
    34681 ttcttacctg ctcagttttg acattatcag caagcacttt tccgaatacc atcagcaacg
    34741 ccttctgatg ttgtcgataa tctttttggg ttcggggggc cagttctgta aatgcagggg
    34801 agtccataaa catgtgccat aatttagcta cggtcattat gttgtggagt tttgcttttt
    34861 ccagttcata attttgccag actttagcta cgctggtttt ccgcactctt cctagcccta
    34921 tacttcttgt acttccttcg ggtttccaca cgtaactgta accattcgat ctaacccgcg
    34981 gtggcagtac attatctttt ttattttttc ttggtcttcc cattgttcag cgcctcaaaa
    35041 tcgggttcag cagaaaccag ttcagatgtt tttggcatcg ttgtcagacc gtgtggaata
    35101 tccctgcgga gaactatggg ttcgttttta gggccgatta caaatgggat gccgtgcagt
    35161 cttaactggt gttgctgttt tgtgtatcgc tcgtatttcg tgatctcttt aatctctgct
    35221 ggagatagag ttaattcgta catgtggtca cgtgccttta cagcatgacc gccgccacta
    35281 tagctggtgg gcggcgatat gggttgaaca tcaaaaatca gcctgactcg ggagcagttt
    35341 ttgccagatg gctgaaacgt atttcgcctg gtaacgtgca tcgttcagag cgttatggcg
    35401 ttcaccttcg aatggaatag tcgttcttgc gtcgaaatcc atcaccagtc ccagagcaac
    35461 catcgttctt acatc
//
LOCUS       DAZFWL020000038        34931 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFWL020000038 DAZFWL020000000
VERSION     DAZFWL020000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.038"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            157..624
                     /gene="slyB"
                     /locus_tag="ACHKNP_RS09885"
                     /old_locus_tag="pgaptmp_001977"
     CDS             157..624
                     /gene="slyB"
                     /locus_tag="ACHKNP_RS09885"
                     /old_locus_tag="pgaptmp_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HGU4704150.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(671..1105)
                     /gene="slyA"
                     /locus_tag="ACHKNP_RS09890"
                     /old_locus_tag="pgaptmp_001978"
     CDS             complement(671..1105)
                     /gene="slyA"
                     /locus_tag="ACHKNP_RS09890"
                     /old_locus_tag="pgaptmp_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HGU4704151.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            1306..1542
                     /locus_tag="ACHKNP_RS09895"
                     /old_locus_tag="pgaptmp_001979"
     CDS             1306..1542
                     /locus_tag="ACHKNP_RS09895"
                     /old_locus_tag="pgaptmp_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HGU4704152.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            1545..2402
                     /locus_tag="ACHKNP_RS09900"
                     /old_locus_tag="pgaptmp_001980"
     CDS             1545..2402
                     /locus_tag="ACHKNP_RS09900"
                     /old_locus_tag="pgaptmp_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGU4704153.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            2402..4414
                     /locus_tag="ACHKNP_RS09905"
                     /old_locus_tag="pgaptmp_001981"
                     /pseudo
     CDS             2402..4414
                     /locus_tag="ACHKNP_RS09905"
                     /old_locus_tag="pgaptmp_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(4415..4936)
                     /gene="sodC"
                     /locus_tag="ACHKNP_RS09910"
                     /old_locus_tag="pgaptmp_001982"
     CDS             complement(4415..4936)
                     /gene="sodC"
                     /locus_tag="ACHKNP_RS09910"
                     /old_locus_tag="pgaptmp_001982"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU4704154.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(5017..5913)
                     /locus_tag="ACHKNP_RS09915"
                     /old_locus_tag="pgaptmp_001983"
     CDS             complement(5017..5913)
                     /locus_tag="ACHKNP_RS09915"
                     /old_locus_tag="pgaptmp_001983"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HGU4704155.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(5962..6201)
                     /locus_tag="ACHKNP_RS09920"
                     /old_locus_tag="pgaptmp_001984"
     CDS             complement(5962..6201)
                     /locus_tag="ACHKNP_RS09920"
                     /old_locus_tag="pgaptmp_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HGU4704156.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            6304..6903
                     /gene="nemR"
                     /locus_tag="ACHKNP_RS09925"
                     /old_locus_tag="pgaptmp_001985"
     CDS             6304..6903
                     /gene="nemR"
                     /locus_tag="ACHKNP_RS09925"
                     /old_locus_tag="pgaptmp_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HGU4704157.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            6940..8037
                     /gene="nemA"
                     /locus_tag="ACHKNP_RS09930"
                     /old_locus_tag="pgaptmp_001986"
     CDS             6940..8037
                     /gene="nemA"
                     /locus_tag="ACHKNP_RS09930"
                     /old_locus_tag="pgaptmp_001986"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HGU4704158.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGANEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            8118..8525
                     /gene="gloA"
                     /locus_tag="ACHKNP_RS09935"
                     /old_locus_tag="pgaptmp_001987"
     CDS             8118..8525
                     /gene="gloA"
                     /locus_tag="ACHKNP_RS09935"
                     /old_locus_tag="pgaptmp_001987"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HGU4704159.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            8628..9275
                     /gene="rnt"
                     /locus_tag="ACHKNP_RS09940"
                     /old_locus_tag="pgaptmp_001988"
     CDS             8628..9275
                     /gene="rnt"
                     /locus_tag="ACHKNP_RS09940"
                     /old_locus_tag="pgaptmp_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HGU4704160.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            9368..13984
                     /locus_tag="ACHKNP_RS09945"
                     /old_locus_tag="pgaptmp_001989"
     CDS             9368..13984
                     /locus_tag="ACHKNP_RS09945"
                     /old_locus_tag="pgaptmp_001989"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HGU4704161.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQECSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVSDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECTARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIIAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(14035..14382)
                     /gene="grxD"
                     /locus_tag="ACHKNP_RS09950"
                     /old_locus_tag="pgaptmp_001990"
     CDS             complement(14035..14382)
                     /gene="grxD"
                     /locus_tag="ACHKNP_RS09950"
                     /old_locus_tag="pgaptmp_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HGU4704162.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            14717..15544
                     /gene="mepH"
                     /locus_tag="ACHKNP_RS09955"
                     /old_locus_tag="pgaptmp_001991"
     CDS             14717..15544
                     /gene="mepH"
                     /locus_tag="ACHKNP_RS09955"
                     /old_locus_tag="pgaptmp_001991"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HGU4704163.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            15672..16253
                     /gene="sodB"
                     /locus_tag="ACHKNP_RS09960"
                     /old_locus_tag="pgaptmp_001992"
     CDS             15672..16253
                     /gene="sodB"
                     /locus_tag="ACHKNP_RS09960"
                     /old_locus_tag="pgaptmp_001992"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HGU4704164.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(16399..17568)
                     /locus_tag="ACHKNP_RS09965"
                     /old_locus_tag="pgaptmp_001993"
     CDS             complement(16399..17568)
                     /locus_tag="ACHKNP_RS09965"
                     /old_locus_tag="pgaptmp_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4704165.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(17734..17823)
                     /gene="cydH"
                     /locus_tag="ACHKNP_RS09970"
                     /old_locus_tag="pgaptmp_001994"
                     /gene_synonym="ynhF"
     CDS             complement(17734..17823)
                     /gene="cydH"
                     /locus_tag="ACHKNP_RS09970"
                     /old_locus_tag="pgaptmp_001994"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HGU4704166.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            18122..19147
                     /gene="purR"
                     /locus_tag="ACHKNP_RS09975"
                     /old_locus_tag="pgaptmp_001995"
     CDS             18122..19147
                     /gene="purR"
                     /locus_tag="ACHKNP_RS09975"
                     /old_locus_tag="pgaptmp_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HGU4704167.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(19144..20076)
                     /gene="punR"
                     /locus_tag="ACHKNP_RS09980"
                     /old_locus_tag="pgaptmp_001996"
     CDS             complement(19144..20076)
                     /gene="punR"
                     /locus_tag="ACHKNP_RS09980"
                     /old_locus_tag="pgaptmp_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HGU4704168.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            20189..21400
                     /gene="punC"
                     /locus_tag="ACHKNP_RS09985"
                     /old_locus_tag="pgaptmp_001997"
     CDS             20189..21400
                     /gene="punC"
                     /locus_tag="ACHKNP_RS09985"
                     /old_locus_tag="pgaptmp_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HGU4704169.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            21691..22839
                     /gene="cfa"
                     /locus_tag="ACHKNP_RS09990"
                     /old_locus_tag="pgaptmp_001998"
     CDS             21691..22839
                     /gene="cfa"
                     /locus_tag="ACHKNP_RS09990"
                     /old_locus_tag="pgaptmp_001998"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HGU4704170.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(22879..23520)
                     /gene="ribE"
                     /locus_tag="ACHKNP_RS09995"
                     /old_locus_tag="pgaptmp_001999"
     CDS             complement(22879..23520)
                     /gene="ribE"
                     /locus_tag="ACHKNP_RS09995"
                     /old_locus_tag="pgaptmp_001999"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGU4704171.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            23735..25108
                     /gene="mdtK"
                     /locus_tag="ACHKNP_RS10000"
                     /old_locus_tag="pgaptmp_002000"
     CDS             23735..25108
                     /gene="mdtK"
                     /locus_tag="ACHKNP_RS10000"
                     /old_locus_tag="pgaptmp_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HGU4704172.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            complement(25149..26405)
                     /locus_tag="ACHKNP_RS10005"
                     /old_locus_tag="pgaptmp_002001"
     CDS             complement(25149..26405)
                     /locus_tag="ACHKNP_RS10005"
                     /old_locus_tag="pgaptmp_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGU4704173.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEERA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            26713..26789
                     /locus_tag="ACHKNP_RS10010"
                     /old_locus_tag="pgaptmp_002002"
     tRNA            26713..26789
                     /locus_tag="ACHKNP_RS10010"
                     /old_locus_tag="pgaptmp_002002"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26747..26749,aa:Val,seq:gac)
     gene            26794..26870
                     /locus_tag="ACHKNP_RS10015"
                     /old_locus_tag="pgaptmp_002003"
     tRNA            26794..26870
                     /locus_tag="ACHKNP_RS10015"
                     /old_locus_tag="pgaptmp_002003"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26828..26830,aa:Val,seq:gac)
     gene            26978..27283
                     /locus_tag="ACHKNP_RS10020"
                     /old_locus_tag="pgaptmp_002004"
     CDS             26978..27283
                     /locus_tag="ACHKNP_RS10020"
                     /old_locus_tag="pgaptmp_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HGU4704174.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            27409..29013
                     /locus_tag="ACHKNP_RS10025"
                     /old_locus_tag="pgaptmp_002005"
     CDS             27409..29013
                     /locus_tag="ACHKNP_RS10025"
                     /old_locus_tag="pgaptmp_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HGU4704175.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDIGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(29025..29837)
                     /gene="ydhT"
                     /locus_tag="ACHKNP_RS10030"
                     /old_locus_tag="pgaptmp_002006"
     CDS             complement(29025..29837)
                     /gene="ydhT"
                     /locus_tag="ACHKNP_RS10030"
                     /old_locus_tag="pgaptmp_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HGU4704176.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(29841..30626)
                     /gene="phsC"
                     /locus_tag="ACHKNP_RS10035"
                     /old_locus_tag="pgaptmp_002007"
     CDS             complement(29841..30626)
                     /gene="phsC"
                     /locus_tag="ACHKNP_RS10035"
                     /old_locus_tag="pgaptmp_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HGU4704177.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(30623..31291)
                     /locus_tag="ACHKNP_RS10040"
                     /old_locus_tag="pgaptmp_002008"
     CDS             complement(30623..31291)
                     /locus_tag="ACHKNP_RS10040"
                     /old_locus_tag="pgaptmp_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU4704178.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(31355..31993)
                     /locus_tag="ACHKNP_RS10045"
                     /old_locus_tag="pgaptmp_002009"
     CDS             complement(31355..31993)
                     /locus_tag="ACHKNP_RS10045"
                     /old_locus_tag="pgaptmp_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HGU4704179.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(32006..34108)
                     /locus_tag="ACHKNP_RS10050"
                     /old_locus_tag="pgaptmp_002010"
     CDS             complement(32006..34108)
                     /locus_tag="ACHKNP_RS10050"
                     /old_locus_tag="pgaptmp_002010"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HGU4704180.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMYASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(34129..34755)
                     /locus_tag="ACHKNP_RS10055"
                     /old_locus_tag="pgaptmp_002011"
     CDS             complement(34129..34755)
                     /locus_tag="ACHKNP_RS10055"
                     /old_locus_tag="pgaptmp_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HGU4704181.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         8375 a         9148 c         8613 g         8795 t
ORIGIN      
        1 atcatcatga atgttttgtt tataattggt tgatcctact ttcattatga tttgctcata
       61 tttggtagaa catgtaacca tggattcaca tatgccatat actttgacca tgagggatgc
      121 ttgcgtggcg tttcatggtg aacaggagat ttttcaatga ttaaacgcgt attggttgtt
      181 tcaatggtag gtctgtctct tgtcggttgt gttaataacg acaccctgtc aggggatgtt
      241 tataccgctt ctgaagcgaa acaagtacag aatgtcagct atggcaccat cgttaacgta
      301 cgtccggtac agattcaggg cggtgatgat tccaacgtta tcggtgcaat tggcggtgct
      361 gttcttggtg gtttcctggg gaatactgtt ggtggcggaa ccgggcgttc tctggctact
      421 gcagcaggcg ctgttgcagg tggcgtagct ggtcagggcg tacagagtgc aatgaacaaa
      481 acgcagggtg tcgagctgga aattcgtaaa gacgatggta ataccatcat ggtggtacag
      541 aaacaaggca acactcgttt ctctccgggc caacgtgtcg tactggccag caatggcagt
      601 caggtgaccg tttctccgcg ctaaataagt ttgcgtgtgg tcaggttact gaccacacgc
      661 ccccttcatt tcaccctttg gcctgtaact caatgatatt atgctcaagt tttgcgatga
      721 gcgtaatcag ttgctccagt tcctctgcgg agatgccatg taatatttcc gcgcgggttt
      781 tgttaataac agcttccatt tcgctgatca gtggctctgc cttttccgtc agtttaatac
      841 gtttagcccg acgatcgctg gcacaagttt gacgcgaaat taaccctttt tcttccagtt
      901 ggtccagagt acggaccagt gatggctgct cgatgccaat cgcttttgcc agttgaattt
      961 gcgactggtc tggaggtaac tgatggatat tgtgtaacgt aacccaatgg gtttgtgtta
     1021 actccagcgg tttcaggcga tggtctatca gagcacgcca tatgcgcacc aaccgtgcca
     1081 gatcagaacc tagtggcgat tccaatttca tccccttata attagcttgc taagatatta
     1141 tgcggctttt agaatagtgt gcagcaattg tattgctaaa acaaatgtat tgctgcattt
     1201 ggttaccgtc agacatattt ttcagaaatt gcgcgtaaat ttttcgcact taaagaatat
     1261 ttattaatct aacgcaatat attcggtcgt aaaggaatct actttgtgaa gtttatgctc
     1321 aatgcaacag gattgccctt acaagatctg gtgttcggtg cgtccgtcta ttttcctccg
     1381 tttttcaaag ccttcgcgtt tggattcgtc atctggcttg tcgtacaccg cctgcttcgt
     1441 ggctggatct acgccggtga catctggcat cccttgttaa tggatttatc gctgtttgcg
     1501 atttgcgttt gccttgctct ggcaatactg attgcgtggt aactatgtca attaaaacaa
     1561 ttaagtattt ctcaacaatc attgtagcga tagttgcggt tcttgccgga tggtggctgt
     1621 ggaattatta catgcaatct ccgtggacac gcgacgggaa aatacgcgca gaaaaggttt
     1681 ctatcacccc acaggtgtcg ggacgtattg ttgagctgaa tataaaagac aaccaactgg
     1741 ttaacgcggg ggatcttctg ctcacgatcg ataaaacgcc ctttcagatc gccgagctga
     1801 acgcacaggc tcagctggcg aaagcgcaat ctgacctcgc aaaagccaat aacgaagcga
     1861 atcgtcgtcg tcatctctca caaaatttta tttctgccga agaactcgat accgctaacc
     1921 tcaatgttaa agcgatgcag gccagtgtta atgccgcaca ggccacgctg aaacagacac
     1981 aatggcaact ggcgcaaacg gaaattcgcg ccccggtgag tggttgggtg actaacctca
     2041 ctacccgcat cggcgattac gctgacaccg ggaaaccgct gtttgccctt gtcgacagcc
     2101 actcgtttta cgtcattggt tattttgaag aaaccaagtt gcgccatatc cgcgaaggtg
     2161 caccagcaca aattaccctc tatagtgata acaaaacgtt acggggtcac gtttccagta
     2221 tcggtcgggc gatttatgat caaagcgttg aaagtgactc aagccttatt ccggatgtga
     2281 aacctaacgt cccctgggtt cgtctcgccc aacgcgttcc cgtccgcttt gcgctggata
     2341 aagttccagg cgatgtcacg ctggtgtcgg gcaccacctg tagcattgcc gtaggtcaat
     2401 aatgaacgca tcgtcatggt ccttgcgcaa tttgccctgg ttcaaggcca cgctggcgca
     2461 atggcgttat gcgttacgca ataccattgc catgtgtctg gcgctgacgg ttgcctatta
     2521 tttgaatctg gatgaaccct attgggcgat gacctcggct gcagtggtta gctttcccac
     2581 cgttggcggt gtcatcagca aaagcctcgg acgcatcgct ggcagtttac tgggagccat
     2641 tgcggcactg cttcttgccg ggcatacgct caatgagccg tggttttttc tattgagcat
     2701 gtcggcgtgg cttggctttt gtacctgggc ctgtgcgcac ttcacgaata acgtcgcgta
     2761 tgcatttcaa ctggcgggct acacggctgc catcatcgcc tttccgatgg ttaatattac
     2821 tgaggccagc cagctgtggg atatcgctca ggcgcgcgtt tgcgaggtga tagtcggtat
     2881 tttgtgcggc ggcatgatga tgatgatcct gcctagcagt tccgatgcta cagccctttt
     2941 aaccgcattg aaaaacatgc acgcccgatt actggaacat gccagtttac tctggcagcc
     3001 tgaaacaaac gatgccattc gtgcagcaca tgagggggtg attgggcaga tactgaccat
     3061 gaatttgctg cgtatccagg ctttctggag ccactatcgt tttcgccagc aaaacgcgcg
     3121 ccttaatgcg ctactccacc agcaattacg tatgaccagt gtcatctcca gcctgcgacg
     3181 tatgttgctc aactggccct caccgccagg tgccacgcga gaaattctcg aacaattgct
     3241 gacggcgctc gccagttcgt aaacagatgc ttacaccgtc gcacgtatta tcgccccgct
     3301 acgcccgacc aacgtcgccg actaccggca cgtcgccttc tggcagcgac tacgttattt
     3361 ttgccgcctt tatctgcaaa gtagtcagga attacatcgt ctgcaaagcg gtgtagatga
     3421 tcatgccaga ctcgcacgga catccgctct ggctcgtcat accgacaacg ccgaagctat
     3481 gtggagcggg ctgcgtacat tttgtacgtt gatgatgatt ggcgcatgga gtattgcatc
     3541 gcaatgggat gccggtgcca atgcattaac gctggcagca attagctgcg tactctactc
     3601 cgccgtcgca gcaccgttta agtcgttgtc actgctgatg cgcacgctgg tgttactttc
     3661 gctattcagc tttgtggtca aatttggcct gatggtccag attagcgatc tgtggcaatt
     3721 tttactgttt ctctttccac tgctggcgac aatgcagctt cttaaattgc agatgcccaa
     3781 atttgccgca ctgtgggggc aactgattgt ttttatgggt tcgtttatcg ctgtcactaa
     3841 tcccccggtg tatgattttg ctgattttct taacgataat ctggcaaaaa tcgttggcgt
     3901 tgcgctggct tggctagcgt tcgccattct gcgtccagga tcggatgctc gtaaaagccg
     3961 ccgccatatt cgcgcgctgc gccgggattt tgtcgatcag ctaagccgcc atccaacact
     4021 gagtgaaagc gaatttgaat cgctcactta tcatcacgtc agtcagttga gtaacagcca
     4081 ggatgcgctg gctcgccgtt ggttattacg ctggggtgta gtgctgctga actgttctca
     4141 tgttgtctgg caattgcgcg actgggaatc gcgttccgat ccgttatcgc gagtacggga
     4201 taactgtatt tcactgttgc ggggagtgat gagtgagcgt ggcgttcagc aaaaatcact
     4261 ggcggccaca cttgaagaat tacagcggat ttgcgacagc cttgcccgtc atcatcaacc
     4321 tgccgcccgt gagctggcgg caattgtctg gcggctgtac tgctcgcttt cgcaacttga
     4381 gcaagcacca ccgcaaggta cgctggcctc ttaattactt aattacacca caggcatagc
     4441 gttcaccgcc accgcccagc ggtttaggtt gatcggacat attatcgccg ccaacgtgga
     4501 ccatcagcgc tttgtctttg atttcatcca gtgatttcag acgaggcgcg atgacggcat
     4561 cggtagcttt gccgtcatta ttgacgacca gtgcaggcag atcgcctaaa tgcccggcac
     4621 cttctggccc ttcatgttta ccggtatttt gtggatcaag atgcccgcct gcggattccg
     4681 cggcgctggc tttgccatct ttggtggctg gctggcagct tcctttggca tgaatatgga
     4741 agccatgttc accggggggt aatgctttca gatcgggcga aaactccaga cctttatcgg
     4801 tttcagtaat ggtgacgcta ccaattgact gccctacccc ttgcgacgtg acgaggttca
     4861 tctcgacttt ttcactggca gcttgtgcgc cggttgcaac aaccagcgcc agaatagcca
     4921 gactaaaacg tttcatagga cctccgttca ttgtgcattc ctgaataagt gtaaaccagt
     4981 gacacatttt tgaacggcgg ctattcctaa aagtgcttac ggtacgtcgt accccagtgc
     5041 cgctttacgg atacgaaacc attgttgacg ggtcattttc agtgtttctg cttcgacagc
     5101 tgcccgtacg cgctcaattt tacctgaacc gataattggc agcggctgcg atggtaaacg
     5161 taatacccag gcgtaaacca cctgttcaat cgagcccgcg tttaactcct ctgccaccac
     5221 agccagttca tcacgcagct gctggaaata atcatcatta aacagacgac caccaccaag
     5281 gcaggaccac gccatcggac gaacacgcag ttgttgtagt tggtcgagcg tgccatccag
     5341 cagtaacggc tgatgcaccg gggatatttc cacctgatta gtggcaaggg taaacggcag
     5401 acgtgattgc aacagggcaa attgcgcagg cgtaaagttc gatacgccaa aatgacgcac
     5461 tttgccgctc tgatgcagat gtttgaacgc gtccgccact tcatcggcat ccattaacgg
     5521 gtctggtcgg tggattaaca gcagatccag atgatcggtc gcgagattaa ttagcgactg
     5581 ttcggcgctc ttaatgatgt gatcgcggtc agtgatgtaa tgaccaatga cgttttcttc
     5641 acgcgcggtc gtcgcgatac cgcatttact gacgatttcc atccgttcac gcaggtgagg
     5701 tgccagtttc agtgcctcgc caaacgccgc ttcgcactga tagccaccat aaatatcagc
     5761 atggtccacg gtggtcacgc cgagatccag atgctcttca ataaaactga ccagctggcg
     5821 ggcggacata ttccagtcca tcaatcgcca gtagcccatc acaaaacggg aaaactccgg
     5881 gccttgcggc gcaatagtaa tacgctgaac cataatcact tcctcttatc agatatgaga
     5941 ggagtatacg caagattagg ttcaaaagag tgatggttgc tccggttcgt ctgatgacgc
     6001 tggcttattt gcgcgtaatt tgcgcattaa tcgctgccga caaaggcgca gcacctcttg
     6061 tttttcgcca tcgctcattt tattccagtt aaaacgctca tcacgactac gaaaacagcc
     6121 gcgacaaaac ccgcgttcgt cagactggca aattccccgg cacgggctct ggacgggaaa
     6181 gaactctaat tgctccgcca cttcgccctc ctcagataag attattacca ttattgaagc
     6241 tgttaatgtc caaagtagca actttgcttg cactagaccg actggtctac tacactccaa
     6301 cgcatgaaca aacacaccga acatgatact cgcgaacatc tcctggcgac gggcgagcaa
     6361 ctttgcctgc aacgtggatt caccgggatg gggctaagcg aattactaaa aaccgctgaa
     6421 gtgccgaaag ggtccttcta tcactacttt cgctctaaag aagcgtttgg cgttgccatg
     6481 cttgagcgtc attacgccgc atatcaccag cgactgactg agttgctgca atccggcgaa
     6541 ggtaactacc gcgaccgcat actggcttat taccagcaaa cactgaacca gttttgccaa
     6601 catggaacca tcagtggttg cctgacagta aaactctctg ccgaagtgtg cgatctgtca
     6661 gaagatatgc gcagcgcgat ggataaaggt gctcgcggcg tgatcgccct gctctctcag
     6721 gcgctggaaa atggccgtga gaaccattgt ttaacctttt gtggcgaacc gctacaacag
     6781 gcacaagtgc tttacgcact atggctgggt gcgaatctgc aggccaaaat ttcgcgcagt
     6841 ttcgagccac tggaaaacgc gctggcccat gtaaaaaaca ttattgcgac gcctgccgtt
     6901 tagcaggcat tttttatcac cagacgaccg ggagccttta tgtcatctga aaaactgtat
     6961 tccccactga aagtgggcgc gatcacggcg gcaaaccgta tttttatggc accgctgacg
     7021 cgtctgcgca gtattgaacc gggtgacatt cctaccccgt tgatggcgga atactatcgc
     7081 caacgtgcca gtgccggttt gattattagt gaagccacgc aaatttctgc ccaggcaaaa
     7141 ggatatgcag gtgcgcctgg catccatagt ccggagcaaa ttgccgcatg gaaaaaaatc
     7201 accgctggcg ttcatgctga aaatggtcat atggccgtgc agctgtggca caccggacgc
     7261 atttctcacg ccagcctgca acctggcggt caggcaccgg tagcgccttc agcacttagc
     7321 gcgggaacac gtacttctct gcgcgatgaa aatggtcagg cgatccgtgt tgaaacatcc
     7381 atgccgcgtg cgcttgaact ggaagagatt ccaggtatcg tcaatgattt ccgtcaggcc
     7441 attgctaacg cgcgtgaagc cggttttgat ctggtagagc tccactctgc tcacggttat
     7501 ttgctgcatc agttcctttc tccttcttca aaccatcgta ccgatcagta cggcggcagc
     7561 gtggaaaatc gcgcacgtct ggtactggaa gtggtcgatg ccgggattga agaatggggt
     7621 gccgatcgca ttggcattcg cgtttcgcca atcggtactt tccagaacac ggataacggc
     7681 gcgaatgaag aagccgatgc actgtatctg attgaacaac tgggtaaacg cggcattgct
     7741 tatctgcata tgtcagaacc agattgggcg gggggtgaac cgtatactga tgcgttccgc
     7801 gaaaaagtac gcgcccgttt ccacggtccg attatcggcg caggtgcata cacagtagaa
     7861 aaagctgaaa cgctgatcgg caaagggtta attgatgcgg tggcatttgg tcgtgactgg
     7921 attgcgaacc cggatctggt cgcccgcttg cagcgcaaag ctgagcttaa cccacagcgt
     7981 gccgaaagtt tctacggtgg cggcgcggaa ggctataccg attacccgac gttgtaatcc
     8041 aacattgcga gcggcgtaaa gccgccgcta tactaaaaca acattttgaa tctgttagcc
     8101 attttgagga taaaaagatg cgtcttcttc ataccatgct gcgcgttggc gatctgcaac
     8161 gctccatcga tttttatacc aaagtgctgg gcatgaaact gctgcgtacc agcgaaaacc
     8221 cggaatacaa atactcactg gcgtttgttg gctacggccc ggaaaccgaa gaagcggtga
     8281 ttgaactgac ctacaactgg ggcgtggata aatacgaact cggcactgct tatggtcaca
     8341 tcgcgcttag cgtagataac gccgctgaag cgtgcgaaaa aatccgtcaa aacgggggta
     8401 acgtgacccg tgaagcgggt ccggtaaaag gcggtactac ggttatcgcg tttgtggaag
     8461 atccggacgg ttacaaaatt gagttaatcg aagagaaaga cgccggtcgc ggtctgggca
     8521 actaatctcc tgccgggcgt gaactcatcg cgcccgcatc tttactgcat cgacaagtaa
     8581 tatttgtcat aatgcgcgct gcaatttatc cgtattaaga gaatcagatg tccgataacg
     8641 ctcaacttac cggtctgtgc gaccgttttc gtggttttta tcctgttgtg atcgatgttg
     8701 aaacagccgg atttaacgcc aaaaccgatg cgctgcttga gattgccgcc atcaccctga
     8761 aaatggatga acaaggctgg ctgatgccgg acaccacatt acatttccac gtcgaaccat
     8821 ttgtcggcgc aaatttgcag ccagaggccc tcgccttcaa cggcattgac ccgaacgatc
     8881 ccgatcgcgg cgcggtcagc gaatacgagg cgctgcacga aatttttaaa gttgtacgta
     8941 aaggtattaa agcgagcggc tgtaaccgcg ccattatggt ggcgcacaat gccaattttg
     9001 atcacagctt tatgatggcc gccgcagaac gcgcctcact gaaacgtaac ccgttccacc
     9061 ctttcgccac ttttgacact gctgcactgg ccgggctggc actcggacaa accgtattgt
     9121 caaaggcttg ccagaccgct ggcatggact tcgacagcac ccaggcgcac tccgcgctgt
     9181 acgacaccga acgcactgct gtgctgtttt gtgaaatcgt caaccgctgg aaacgtctgg
     9241 gaggctggcc gctacctgcc gccgaagagg tgtaatcgag tcgatgcctg atgacatgca
     9301 atgattcagg catctatagt gaggctattc cacgcatcaa gcatgatatt cacggggaat
     9361 agcgttaatg gcagataatc cagacccttc atcgctcctg ccggacgtgt tttcaccggc
     9421 gacccgcgac tggtttcttc gcgcctttaa acagccgacc gctgtccagt cgcaaacctg
     9481 gcatgtggcg gcgcgaagcg aacatgcgct ggtgattgca ccgaccggct ccgggaaaac
     9541 gctggcagca tttctctacg ccctcgatcg gctcttccgc gaaggcggcg aagatacccg
     9601 cgaggcgcat aagcgtaaaa cctcacgcat cctctatatt tcaccgataa aagctctggg
     9661 caccgacgtt cagcgcaact tgcagctccc gttgaagggt attgccgatg aacggcggcg
     9721 gcgcggcgaa acggaagtca atcttcgcgt agggatccgt actggcgata cgcctgcaca
     9781 ggaatgcagc aaactcaccc gtaatccgcc ggatattctg atcaccacac ccgaatcact
     9841 ctatctgatg ctgacctccc gcgcgcgcga aacgctacgc ggcgtcgaaa cggtaattat
     9901 tgatgaagtc cacgcggtag cgggcagtaa acgtggtgcg catctggcgt taagtctgga
     9961 gcggctcgat gcgctgctcc acacctcagc acagcgaatt ggcctttctg ccactgtgcg
    10021 ctcagccagc gatgtggcag catttcttgg tggcgatcgc ccggttacgg tagtcaaccc
    10081 gcccgcaatg cgccatccgc agatacgaat tgtcgtaccg gtcgccaata tggatgatgt
    10141 ctcatcggtc gccagcggca ccggcgaaga cagccatgcc ggccgggaag gctccatctg
    10201 gccatatatt gaaacgggta tccttgatga agtgttgcgc catcgctcga ccattgtctt
    10261 tactaattca cgtgggctgg cggaaaaact gacggcacga ttaaatgagc tttacgccgc
    10321 acgcttacag cgttccccgt ctatcgccgt tgatacggcc catttcgagt cgacctccgg
    10381 cgcaacctct aaccatgtac aaagtagcga cgtttttatt gcccgctcac accacggctc
    10441 cgtctctaaa gaacaacgag caatcaccga acaggcgctg aaatcgggtg aattacgctg
    10501 cgtggtcgca acctccagtc tcgaactggg gattgatatg ggcgcggtgg atctggtgat
    10561 tcaggtggca acgccgcttt ctgttgccag tgggttacaa cgcattggtc gcgccggaca
    10621 tcaggttggc ggtgtatcta aagggctgtt tttccctcgt acccggcgtg acttagtcga
    10681 ttccgcagtc attgtagagt gtatgttcgc aggcaggctg gaaaacctga caccgccgca
    10741 taatccactc gacgtccttg cgcagcaaac cgttgccgcc gcggcgatgg atgcattaca
    10801 ggtagacgaa tggtactccc gcgtacgccg tgccgcaccg tggaaagatc tgccaagacg
    10861 tgtttttgac gccacgctgg atatgctttc cgggcgctat ccctctggcg atttttctgc
    10921 ttttcgcccc aaactggtct ggaacaggga gaccgggata ttgaccgccc gacctggcgc
    10981 tcaattgttg gcggttacca gcggcggcac cattccggat cgtggcatgt atagcgtgtt
    11041 attacccgaa ggtgaagaac aggccggttc gcggcgggtg ggtgaactgg atgaggagat
    11101 ggtatatgag tcgcgggtga gcgacattat cactctcggc gctacctcat ggcggatcca
    11161 gcaaatcacc cgcgatcagg tgattgtgac tcctgctccg ggtcgttctg cccggctccc
    11221 cttctggcgt ggtgaaggta acggacgtcc ggctgaatta ggcgagatga tcggcgattt
    11281 tcttcattta ctggcggatg gcgcgttctt ttccgggact attcccccgt ggctggcaga
    11341 agaaaatacg aacgccaata ttcaggggtt gattgacgag caacgcaacg cgacgggcat
    11401 cgttccgggg agtcgccatc tggtcctcga acggtgccgt gatgaaattg gcgactggcg
    11461 tattattttg cactctccct atggaagacg ggtgcatgaa ccctgggcgc tggcgattgc
    11521 cgggcgaata catgccctat ggggcgctga cgcgtctgtg gtcgccagtg atgacggcat
    11581 tgttgcgcgc attcctgaca ctgatggtaa attgcccgac gccgcgattt ttttgtttga
    11641 accagaaaag ttgctgcaaa ttgtccgcga ggcggtaggc agctcggcac ttttcgccgc
    11701 ccgttttcgc gaatgcaccg cgcgggcatt attaatgccg gggcgcactc cgggccatcg
    11761 caccccgctt tggcaacaac ggctgcgcgc cagtcagttg ctggaaatcg ctcagggata
    11821 tccggatttt ccggtcattc tcgaaaccct acgcgaatgt ctgcaagatg tttatgatct
    11881 tcccgcactg gaacgtttga tgcgtcgcct gaacggtggc gaaattcaaa tatccgatgt
    11941 aacgaccact acgccctcgc ctttcgccac aagtttattg ttcggctatg tcgcggaatt
    12001 tatgtaccag agcgacgccc cgctggcaga gcgccgggca tccgtactgt cgctggacag
    12061 cgagttactg cgcaatctac tcggacaggt cgatccgggg gaattactcg acccgcaggt
    12121 cattcgccag gtggaagaag agttgcaacg actggctcct ggcagaagag cgaaaggtga
    12181 agaaggattg ttcgacctgc tgcgcgaact ggggccaatg accgttgaag acctggcgca
    12241 acggcataca ggcagcagtg aagaggttgc gtcgtatctg gaaaatcttc ttgcagtaaa
    12301 acgaatcttc ccagcgatga ttagcggaca ggagcgtctt gcctgtatgg atgatgccgc
    12361 caggctgcgt gatgccctcg gcgtacgact accagagtca ttgccagaga tttatttaca
    12421 tagagtcagt tacccgcttc gcgacctctt tctgcgctat ctccgggctc atgctctggt
    12481 cacggctgaa caactggctc atgagtttag tctcggtatt gccattgtcg aagagcagct
    12541 tcagcaactg cgtgaacagg gtctggtgat gaatctgcaa caagacatct gggtgagcga
    12601 tgaagtattt cgtcgtctgc gtttgcgctc gctgcaagcc gccagagaag cgacgcgtcc
    12661 cgttgcagcc acgacctatg cgcgattgct gctggaacgt cagggcgtat tacccgccac
    12721 cgatggtagc ccggcgctct ttgcctcaac atcgccaggc gtttatgagg gcgtagatgg
    12781 cgtgatgcgg gtgatcgaac agcttgccgg agtcggttta cccgcctcac tctgggaaag
    12841 ccagatcctg cctgcccgcg tacgcgacta ttcgccagaa atgctcgatg aattactggc
    12901 aaccggagcg gttatctggt cgggacaaaa aaagctgggt gaagatgacg gcctggtggc
    12961 actgcatcta caggaatatg ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa
    13021 tcgttcggcg ctgcagcaag cgataatcgc tgttctggct gacggaggag cctggtttgc
    13081 acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc tctctgccct
    13141 gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca tttgggcacc
    13201 gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg
    13261 ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat cttacaacac
    13321 accaaatctg gctggacgct ggtcgttatt gcaggtggag ccactaaacg ataccgaaag
    13381 gatgctggcg ctggcggaaa atatgctcga ccgctacggc atcatcagtc gtcaggcggt
    13441 gatagccgaa aatatccctg gcgggtttcc atcgatgcaa acgctttgtc gaagtatgga
    13501 agactccggg cgaattatgc gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc
    13561 tgaacgtctg actattgacc gattgcgcga tctggcgaca caagccacgc aaacgcgcca
    13621 ctatacacca gtggcgctct ctgccaacga tccggctaat gtgtggggaa atcttctgcc
    13681 ctggcctgca catccggcaa cgctggttcc aacgcgtcgg gcgggtgcgc tggtggtcgt
    13741 ttctggcggc aaattgttac tctatctggc gcaaggtggc aaaaaaatgc tggtctggca
    13801 ggaaaaagag gaattactcg ccccagaggt tttccacgcg ctgactaccg cactgcgtcg
    13861 cgaaccacgg ctgcgcttta cgctaacaga agtgaatgat ctaccggtcc ggcaaacgcc
    13921 gatgtttacg ctgctgcgtg aggcgggatt ttcaagttcg ccacaagggc tggattgggg
    13981 atagagaaag gactgacgga tgcccgttcg catccgtcag tattgcagga cggattattc
    14041 cgcgtccggc tcttcagact tgtatttagc ggcagtttct ttgatcagct gctgcagttc
    14101 gccacgctga tacatttcga tcacgatatc acaaccgcct accagctcgc cgtcaaccca
    14161 cagttgcggg aaggtcggcc agttagcata tttaggcagt tccgcacgaa tgtccgggtt
    14221 ctgcagaata tcaacatagg caaaacgttc gccacatgcg gcaagcgcct ggactgcctg
    14281 ggcagagaaa ccgcagctcg gcagtttcgg tgaacctttc atgtacagca ggatcgggtt
    14341 ttcagcaatc tggcgttgga ttttttcgat agtggtgctc attgtcttgc ttcctcaaac
    14401 ttctttacgg cagtaatctg acattgtagc gggtcagtgc ggcatcggaa aataacattt
    14461 tcatcacgct tttgctattt tatccctttg ctctatttgt tgcattttaa atattcgttt
    14521 cttcccagat gctcggcaaa ctggcgtatt tataaccatt tttagctttc actgctgcat
    14581 ttttttgcgc tcgccaacga aacgtatttt ttaacaataa aagctattaa ctttctcttc
    14641 ttctatgcat tagaatcatc aagttttgta aatcagacgc aggcatgata gacctgcctt
    14701 aacagaggga cgctcagtgg cgcggataaa ccgtatttcg atcacgctct gtgctttact
    14761 ttttaccacc ctgcctttaa cgcctatggc ccatgcttca aagcaagcca gggagagttc
    14821 tgctaccact catatcacca aaaaagcaga taaaaagaaa agcacggcaa ccaccaaaaa
    14881 gacccaaaaa gccagccaga aaacagcgaa aaaagccgcc agtaaaagta cgaccaaaag
    14941 caaaaccgct tcttccgtta aaaaatcttc cattaccgct tctaaaaacg ccaaaactcg
    15001 cagcaaacac gccgtcaata aaacggcctc agccagcttt accgaaaagt gtaccaaacg
    15061 taagggctat aaatcgcatt gtgtgaaagt caaaaatgcc gcgtcaggaa ctcttgccga
    15121 cgcgcacaaa gcgaaggtgc aaaaagctac gaaagtggca atgaataaac tgatgcagca
    15181 aattggtaag ccatatcgtt ggggtggcag ctcaccgcgt accggttttg attgcagcgg
    15241 cctggtttat tacgcttata aagatttggt gaaaattcgt attccgcgta cggcgaatga
    15301 aatgtatcac ctgcgtgatg cagcgccaat cgaacgtagt gaactgaaaa acggcgacct
    15361 ggtctttttc cgtactcagg gacgcggcac agccgatcat gtcggcgtgt atgtcggcaa
    15421 cggcaaattt attcagtcac cgcgcacagg tcaggaaatt caaatcactt ctctcagtga
    15481 agactactgg cagcgccact atgttggcgc tcgtcgggta atgaccccaa aaacacttcg
    15541 ctaaaacttt accctgttgt tacggcaaca gggtaagttc atcttttgtc tcacctttta
    15601 atttgctacc ctatccatac gcacaataag gctattgtac gtatgcaaat taataataaa
    15661 ggagagtagc aatgtcattc gaattacctg cactaccata tgctaaagat gctctggcac
    15721 cgcacatttc tgcggaaact atcgagtatc actacggcaa gcatcatcag acttatgtca
    15781 ctaacctgaa caacctgatt aaaggtaccg cgtttgaagg taaatcactg gaagagatta
    15841 ttcgcagctc tgaaggtggc gtattcaaca acgcagctca ggtctggaac catactttct
    15901 actggaactg cctggcaccg aacgccggtg gcgaaccgac tggaaaagtc gctgaagcta
    15961 tcgccgcatc ttttggtagc tttgccgatt tcaaagcgca gtttactgat gcagcgatca
    16021 aaaactttgg ttctggctgg acctggctgg tgaaaaacag cgatggcaaa ctggctatcg
    16081 tttcaacctc taacgcgggt actccgctga ccaccgatgc gactccgctg ctgaccgttg
    16141 atgtctggga acacgcttat tacatcgact atcgcaatgc acgtcctggc tatctggagc
    16201 acttctgggc gctggtgaac tgggaattcg tagcgaaaaa tctcgctgca taataactga
    16261 tggcaaatgc agcattgcct gatgcgcttc gcttaccatg ccaacgcgga tgaggcgtta
    16321 acagcgcatt cggcaagata aatcgcacgt tgtcagcaac tgtaacgcag aaggttatcc
    16381 ttctgcgttt ttgtttaatt agctgttagc aacgcaaact gtttcaggtt gttttctggc
    16441 tgacataaac accagcaata atgccagtcc cgcgacaatc gctcccatca ccggcacaaa
    16501 gctgtatccc agcccagcgg aaattaccgc accaccagca gctgctccca gcgcatttcc
    16561 aagattaaag gcaccaatat tgactgatga agacagacct ggcgcttcac tggcgacacg
    16621 catcacgcgc atctgtaacg gcggtacgac cgcaaaggtt gctgcgcccc acaccaccat
    16681 gctaatagct gcgccgaact cattgcgggc caggaacggg attgccagca taatcaccat
    16741 caacaacaac aaaaagcctt tcaacgtgcc gttaactgaa cgatctgcca gtttgccgcc
    16801 gagatagtta ccgatagaga atccgacacc aatcagcacc agcattgccg tgacgaacac
    16861 tggtgttgcg tgggtaatac tttgtagtac cggagagata taggtgtaga gagtaaacat
    16921 tgcaccagct cccagtaccg tcgtcagcaa tgcagacagc acctgcggac gcattaatac
    16981 cgccagctct tttttcactt caggtcgtgc ccctgcacca cctttaggta atgagaagaa
    17041 cagacttacc attgaaatca ctcccagccc cgccgttgcc agaaatgaca tccgccagcc
    17101 gatggtttca cccaaccagg tcgccgccgg cacgccaccg atatttgcca gggttaaccc
    17161 cataaacata gtggcaactg cgctggcctg tttatgtttt ggcaccacgc ttgcggccac
    17221 gactgaaccc aaaccaaaaa atgctccgtg attcaggctg gtcaaaatgc gtgaaagcat
    17281 cagggtcata taatccggcg cgatggcgga aagtacgttg ccgagcgtga aaattgccat
    17341 caggaaaatc aacgcactgc ggcgggcacg atgagaaagt agaagcgtca tcagcggcgc
    17401 gccaaccatt acgccaactg cataggcact gattaacatt ccggcagcgg gaatcgagac
    17461 atccacaccg cgcgcaatga cgggcaacaa acccattgga gagaactccg ttgtcccgat
    17521 accaaacgcg ccaatcgcca gcgccagcaa cggatagtta attttcatgc cttatctcca
    17581 cctcttcgcg tcattacgcg atattcatta aagtggcgaa agcatgacag caatcacaaa
    17641 aaaatgaaaa taacaaaaag agaaaacact tttgccattt tgctaacaaa caggaaggag
    17701 atgcgaggga gaacgcgctc cctcgagagg aaatcagtgc agcgcggcag tcaaacccac
    17761 ggccacgatc aaaccgagga cgataatcgt tgttaccagt gaaaatttaa ggtcggtgct
    17821 catcaagttt tctccttttt tattaccaca caaatagtga tattacgcat ttttacacac
    17881 tgtgatgaaa aaatctcccg tcatttataa tgataagtgt ttttaccact tccccttttc
    17941 gtcaagatcg gccaaaattc cacgcttaca ctatttgcgt actggccatt gaccccttcc
    18001 tgacgctccg tgtcgttttt ccggcgtacc gcaacacttt tgttgtgcgt aaggtgtgta
    18061 aaggcaaacg tttaccttgc gattttgcag gagctgaagt tagggtctgg agtgaaatgg
    18121 aatggcaaca ataaaagatg tagcgaaacg agcaaacgtt tccactacaa ctgtgtcaca
    18181 cgtaatcaac aaaacacgtt tcgtcgctga agaaacgcgc aacgccgtgt gggcagcgat
    18241 taaagaatta cactactccc ctagcgcggt ggcgcgtagc ctgaaggtta accacaccaa
    18301 gtctatcggt ttgctggcga ccagcagcga agcggcctat tttgccgaga tcattgaagc
    18361 ggttgaaaaa aattgcttcc agaaaggtta caccctgatt ctgggtaatg cgtggaacaa
    18421 tcttgagaaa cagcgggctt atctgtcgat gatggcgcaa aaacgcgtcg atggtctgct
    18481 ggtgatgtgt tctgagtacc cagagccgtt gctggcgatg ctggaagagt atcgccatat
    18541 cccaatggtg gtgatggact ggggtgaagc aaaagctgac ttcaccgatg cggtcattga
    18601 taacgcgttc gaaggcggct acatggccgg gcgttatctg attgaacgcg gtcaccgcga
    18661 aatcggcgtc atccccggcc cgctggaacg taacaccggc gcaggccgcc ttgccggttt
    18721 tatgaaggcg atggaagaag cgatgatcaa ggtgccggaa agctggattg ttcagggtga
    18781 ctttgaaccc gaatctggtt atcgcgccat gcagcaaata ctgtcgcagc cgcatcgccc
    18841 tactgccgtc ttctgtggtg gcgatatcat ggcaatgggc gcactttgtg ctgctgatga
    18901 aatgggtctg cgcgtcccgc aggatgtttc gctgatcggt tatgataacg tgcgcaacgc
    18961 ccgctatttt acgccggcgc tgaccacgat ccaccagcca aaagattcgc tgggtgaaac
    19021 agcgttcaac atgctgttgg atcgtatcgt caacaaacgt gaagaaccgc agtccattga
    19081 agtgcatccg cgcttgattg aacgccgctc cgtggctgac ggcccgttcc gcgactatcg
    19141 tcgttaatca cccgttgcgg gagtctcttc cggctcccgc agccactcct tattcagcgt
    19201 ctcactatcg ccgagatact caagcagcca ggttaacgca ggcgacatat cattttgctg
    19261 ccatgtcaga caacatgccg aatccggaaa ggggttttcc agttctaatg ccacccactt
    19321 cccctcatta agccacggtt tggcgaaatg tgttggcacc atccctatgc ataatcctgc
    19381 cgagatacag gttgccgatg attcccagtc aggcacgaca actctttttt ggttatccag
    19441 caaccaggta atacgtttag gtagcgttcg cgaggtgtct tcgcgcacca acgacggcca
    19501 gttgcgcaac gtatcatcgc tgaacgggcc atccatcaac gccagcgggt ggtggctggc
    19561 aacaacgcaa ctccagctta gcatccccat atcccggaag gcataacgac cgcctaccgg
    19621 aatcgcgcgt gttgcgccaa tcgccagttc cacgcgccca tcggaaagcg catcccagac
    19681 accgttaaac acttcctgaa agaccagaag ttcgacgtcg tcaaaatggc gataaaaatc
    19741 aacgatcatc tgccgtgtac gttctggcct gacaatatta tccactgcga tagctaactg
    19801 accgcgccag ccgttcgcta tctgctgaca ttgctggcgg gtgatctgca tttttttgac
    19861 aacagagcgc ccttctttga gaaaccacgc tccagcagcg gtcagctcca catcacggtg
    19921 ccgtcgttca aagagcggta ccgccagcca ctcttccagc tgacgcacgg tatagctgac
    19981 cgcagaagga acgcgatgca gctcctgtgc cgcagcgcta aaactaccat tacgcgctac
    20041 cgcatcaaca acttcgagtg aatattctga ccacatagtc tgcctgcaaa atttttgaaa
    20101 ccagtcatca aatattaccg tttcacaaca ctaatttcac tccctacact ttgcggcggt
    20161 gtttaattga gagatttaga gaatatacat gcaacctggg aaaagatttt tagtctggct
    20221 ggcgggtttg agcgtactcg gttttctggc aaccgatatg tatctgcctg ctttcgccgc
    20281 catacaggcc gacctgcaaa cgcctgcgtc tgctgtcagt gccagcctta gtctgttcct
    20341 tgccggtttt gccgcagccc agcttctgtg ggggccactc tccgaccgtt atggtcgtaa
    20401 accggtgtta ttaatcggcc tgacaatttt tgcgttaggt agtctgggga tgctgtgggt
    20461 agaaaacgcc gctacgctgc tggtattgcg ttttgtacag gctgtggggg tctgcgccgc
    20521 ggcggttatc tggcaagcat tagtgacaga ttattatcct tcacagaaag tcaaccgtat
    20581 ttttgcgacc atcatgccgc tggtgggtct gtctccggca ctggctcccc tgttaggaag
    20641 ctggctgctg gtccattttt cctggcaggc gattttcgcc accctgtttg ccattaccgt
    20701 ggtgctgatt ctgcctattt tctggctcaa acccacgacg aaggcccgta acaatagtca
    20761 ggatggtctg acctttaccg acctgctacg ttctaaaacc tatcgcggca acgtgctgat
    20821 atatgcagcc tgttcagcca gtttttttgc atggctgacc ggttcaccgt tcatccttag
    20881 tgaaatgggc tacagcccgg cagttattgg tttaagttat gtcccgcaaa ctatcgcgtt
    20941 tctgattggt ggttatggct gtcgcgccgc actgcagaaa tggcaaggca agcagttatt
    21001 accgtggttg ctggtgctgt ttgctgtcag cgtcattgcg acctgggctg cgggcttcat
    21061 tagccatgtg tcgctggtcg aaatcctgat cccattctgt gtgatggcga ttgccaatgg
    21121 cgcgatctac cctattgttg tcgcccaggc gctgcgtccc ttcccacatg caaccggtcg
    21181 cgccgcagcg ttgcagaaca ctctgcaact gggtctgtgc ttcctcgcaa gtctggtagt
    21241 ttcctggctg atcagtatca gcacgccatt gctcaccacc accagcgtga tgttatcaac
    21301 agtagtgctg gtcgcgctgg gttacatgat gcaacgttgt gaagaagttg gctgccagaa
    21361 tcatggcaat gccgaagtcg ctcatagcga atcacactga tctatatcga tatacttata
    21421 cttaggctgc taacaaaatt ttgttgtatg attgaaatta gcggcctata ctaatttcga
    21481 gttgttaaag ctacgataaa tattatgttt ttacggggac aggatcgttc ccgactcact
    21541 atggatggtc atttcggcaa gggttcctcc tttccctctg ttctacgtcg gattatagac
    21601 tcgcggtttt ttctgcgaga tttctcacaa agcccaaaaa gcgtctacgc tgttttaagg
    21661 ttctgatcac cgaccagtga tggagaaact atgagttcat cgtgtataga agaagtcagt
    21721 gtaccggatg acaactggta ccgtatcgcc aacgaattac ttagccgtgc cggtatagcc
    21781 attaacggtt ctgccccggc ggatattcgt gtgaaaaacc ccgatttttt taaacgcgtt
    21841 ctgcaagaag gctctttggg gttaggcgaa agttatatgg atggctggtg ggaatgtgac
    21901 cgactggata tgttttttag caaagtctta cgcgcaggtc tcgagaacca actcccccat
    21961 catttcaaag acacgctgcg tattgcaggc gcccgtctct tcaatctgca gagtaaaaaa
    22021 cgtgcctgga tagtcggcaa agagcattac gatttgggta atgacttgtt cagtcgcatg
    22081 cttgatccct tcatgcaata ttcctgcgct tactggaaag atgccgataa tctggaatct
    22141 gcccagcagg cgaagctcaa aatgatttgt gaaaaattgc agttaaaacc agggatgcgc
    22201 gtactggata ttggctgcgg ctggggcgga ctggcgcact acatggcatc taactatgac
    22261 gtaagcgtgg tcggcgtcac catttctgcc gaacagcaaa aaatggctca ggaacgctgt
    22321 gaaggcctgg atgtcaccat tttgctgcaa gattatcgtg acctgaacga ccagtttgat
    22381 cgtattgttt ctgtggggat gttcgagcac gtcggaccga aaaattacga tacctatttt
    22441 gcggtggtgg atcgtaattt gaaaccggaa ggtatattcc tgctccatac tatcggttcg
    22501 aaaaaaaccg atctgaatgt tgatccctgg attaataaat atatttttcc gaacggttgc
    22561 ctgccctctg tacgccagat tgctcagtcc agcgaacccc actttgtgat ggaagactgg
    22621 cataacttcg gtgctgatta cgatactacg ttgatggcgt ggtatgaacg attcctcgcc
    22681 gcatggccag aaattgcaga taactacagt gaacgcttta aacgaatgtt tacctattat
    22741 ctgaatgctt gtgcaggtgc tttccgcgcc cgtgatattc agctctggca ggtggtgttc
    22801 tcacgcggtg ttgaaaacgg ccttcgagtc gctcgctaaa ggctattcta tcgccccctc
    22861 ttcgggggcg agttcagatc aggcttctgt acctggttga ttcatggcat tttctcgtgc
    22921 cgccagcaca cgttctaccg tatctaccac tgcctgagtt tgtggatcaa tttcaatgtt
    22981 gacgcgtgcg ccgagttttt tcttcccaag agtcgtgcgt tccagtgttt ccggaattaa
    23041 atggacgcaa aaacgcgttg gcgtgacttc gccgacggtc aggctaatac cgtcgatgcc
    23101 aataaatcct ttgtacagaa tatatttcat caactgacta tcctggactt taaaccagat
    23161 ctggcgatta ttttctgagg ttaatatttt cgccacttca gcagtggtca taatatgacc
    23221 tgacattaag tgtccgccaa tttcatcact gaatttcgcc gcacgctcaa cgtttaccca
    23281 atcccccact tttaaatcgc caagattggt aatgcgtaac gtttctttca tcaggtcaaa
    23341 actgacatgg ttgccgttaa tttccgtcac ggtcaggcag caaccgttat gcgccacgga
    23401 cgcaccggtt tccaggccgt ccagcatgtg gtcgggtaac tccaccacat gcgtacgaaa
    23461 atttggtttc tcgtcaatcg acaccagttt tgcggtgccc tgtacaatcc ccgtaaacat
    23521 acttacaact cctgaaatca gttaagacat tctgttcagc acaatagcag gtggcaaacg
    23581 cccttaccag tgaaggggta agaatgacta ttttttcact ggagaattaa taaatcctcg
    23641 ctacaataga ctgaatttcc cctgcttctt ctttttgctg cccattcagg cggcttttta
    23701 gtctctcata taactacaaa taaaaggtgt tcacgtgcag aagtatatca gtgaagcgcg
    23761 tctgttatta gcattagcaa tcccggtgat tctcgcgcaa atcgcccaaa ctgcgatggg
    23821 ttttgtcgat accgtgatgg cgggcggcta tagtgccacc gacatggcgg cggtcgctat
    23881 cggtacttct atctggcttc cggcgatcct ctttggtcac ggactgctgc tggcattaac
    23941 gccggttatc gcgcaattaa atggttccgg tcgacgtgag cgcattgcgc atcaggtgcg
    24001 acaaggtttc tggctggcag gttttgtttc cgttctcatt atgctggtgc tgtggaatgc
    24061 aggttacatt atccgctcca tggaaaacat cgatccggct ctggcggaca aagccgtggg
    24121 ttatctgcgt gcgttgttgt ggggcgcgcc gggctatctg ttcttccagg ttgcccgtaa
    24181 ccagtgtgaa ggtctggcaa aaaccaagcc gggtatggta atgggcttta tcggcctgct
    24241 ggtgaacatt ccggtgaact atatctttat ttatggtcat ttcggtatgc ctgagctcgg
    24301 tggcgttggt tgtggcgtgg ctactgcggc ggtgtactgg gttatgttcc ttgccatggt
    24361 ttcttacatt aaacgcgccc gctccatgcg cgatattcgt aacgaaaaag gcaccgcgaa
    24421 acccgatcct gcggttatga aacgactgat tcaactcggt ttgccgattg cgctggcact
    24481 gttctttgaa gtgacactgt ttgctgtcgt ggctctgtta gtgtctccgc tcggtattgt
    24541 tgatgtcgca ggacaccaga ttgccctgaa ctttagttca ctaatgttcg tgcttcccat
    24601 gtcgctggcg gcagcggtaa ctatccgcgt aggttatcgt ctgggtcagg gctcaacgct
    24661 ggatgcgcaa accgctgcgc ggaccgggct tatggtgggt gtctgtatgg caaccctgac
    24721 ggccatattc acggtttcac tgcgggagca aatcgccctg ttgtacaacg acaatcccga
    24781 ggttgtaacg ctggctgcgc atttgatgtt gctggcggcg gtatatcaga tttctgactc
    24841 gatccaggtg attggcagtg ggattttgcg tggttataaa gatacgcgtt ccattttcta
    24901 tattaccttt acggcttact gggtgctggg cttgccaagc ggctatattc tggcactgac
    24961 cgatctggtc gttgaaccta tggggccagc aggcttctgg ataggcttta ttattggcct
    25021 gacgtcggca gccattatga tgatgctgcg tatgcggttc ctgcaacgtc tgccgtcagc
    25081 catcattctg caacgagcgt cccgctaata aagacaaggc gcaaccttca cgggttgcgc
    25141 ctgtgttttt acgcaggctg gagcgttgcg ccaatcccgt cttcgtctgg ctgtagtttc
    25201 agtgcgttac acagagtttg ccgactggca tctgttatct caacgcgtat atccccggta
    25261 acttcccctt tcacattgat gaagtcaggt gagatttctt tcgcactggt ttgcaggcaa
    25321 atcgctccct ctccgcgcag gcgtttcact ttcagttgca catgcgttgc cgcaccagtg
    25381 atcagccaca gttttccgcc attcagattg atgttaccaa cactaaaagt gacactgtca
    25441 ccttttaccg tactgccgtc gagaatcaac acaccgccct gctgtacagt aatcgttgat
    25501 tgttgcagat ctgcaccata gccaataatc attgaagcgc cgtccagtaa tgcaacactc
    25561 ccggtcagtt caaccggtgc gcgctcttcc tctacgacaa aattttcgtc cgcgctggtg
    25621 ggtgacaaca ggatcactgt tccaccttgt attaccgcat tttccagcgt accggcatgc
    25681 acttcagccc gaccacccac attcacggac aggttttgcg tcttaccgga actgtagagc
    25741 tgaaggccat ccgtccccag acgataaatg gatccattct caacaatagt gtcagagtct
    25801 tcaccaccga gattattcat ggtgccacca gcaagcaacg tatcactggc tttcccggcc
    25861 agcataaaaa cgccgccctg attaatttca caattgctgg cttccccacc tggatacacc
    25921 aacaattcac caccttcatc taccacgaca gctgaggttg tcccattgac caacaggccg
    25981 ccctcttgat cgagaatgat tttttccgcg cgatgtcctt ccagtacacg caggttaccg
    26041 ccattttcca gcaacaaacc gcaggcataa ccctgatcaa cgctgaattc accttcggga
    26101 tggcggccat taaccgtagc gagcgtagag agactaattg cgcctccctc ggactgaaca
    26161 atatctgtgg caatggcacc gtcgctgacc gatagcgtgc cattgttacc gtactgtgtg
    26221 catttggcga tcccaccggt tgccactttc aaccagccag cccccgtgac cacggtattt
    26281 tcggctcttc cgccagcttc aacaatcaac tcgccttcag taagttgcgt cgcgccaatg
    26341 acctcgccgg tcttaacaat ttgcacattc ccgtcgctca tcaagttttt cgcatcagat
    26401 cccatgattt attcctttgc tgcatctgtg tgcctttatt gctacctaag tgtaaaggct
    26461 acggaggatt tatccacgac agatttgaga tggtggcaaa caactctgtt taaactctga
    26521 tacacgaatt attgggttgt atcagatgta aatgcgatcc tgaataaaaa tcgcccttgc
    26581 aaatcaacaa aatatagcca atttgcttaa aagctctgca aacggtcgaa atagcgcaga
    26641 aaattacgtt ttgcctcttg ccaccttccc actctgccgc taatattcgt ccccgttgtc
    26701 acctacaacg ttgcgttcat agctcagttg gttagagcac caccttgaca tggtgggggt
    26761 cgttggttcg agtccaattg aacgcaccat cctgcgtccg tagctcagtt ggttagagca
    26821 ccaccttgac atggtggggg tcggtggttc gagtccactc ggacgcacca gattttctta
    26881 atctggtctt ctcctttttt cctctgtttc ttctctgtat ccaatacgtt aaaagattta
    26941 cactgtcttc gtatgcgtta tcagaaggag aattgctatg gcaactttgt tacaacttca
    27001 ttttgctttt aatggcccat ttggcgacgc aatggctgag cagcttaaac cacttgctga
    27061 gtcgattaat caggaacctg gttttctgtg gaaggtatgg acagaaagtg aaaagaacca
    27121 cgaagccggt gggatctatc tttttactga tgaaaaaagc gcccttgcct atctggaaaa
    27181 acatactgcc cgacttaaaa atctcggcgt tgaggaagtt gtcgccaaag ttttcgatgt
    27241 caatgagcca ctgagtcaaa tcaatcaggc aaaactcgcc tgacagaatt taatcaaggg
    27301 cggttagcgc ccttttcata tctgtctgaa atttctcaaa ttctaaaaat ctcaaccaaa
    27361 cttatctgat aacactaaat tcgaaagaat gcgtacaggt aagtaacaat gaaaaaaatt
    27421 gctattgtgg gtgccgggcc tacggggatc tacaccttat tctcgcttct acagcaacaa
    27481 actccacttt ctatttctat cttcgagcag gctgacgagg ccggtgtcgg gatgccatac
    27541 agtgatgagg aaaactcaaa aatgatgctg gcaaatattg ccagtattga aataccgccg
    27601 attaattgta cgtatctcga atggctacaa aagcaagaag ccagccatct ccagcgttat
    27661 ggcgttaaaa aagaaacctt gcacgatcgt cagtttttac cgcgaattct gctgggcgaa
    27721 tatttccgcg atcaattttt acgattagta gaccaggcac gacagcaaaa atttgcagtg
    27781 gctgtttatg aatcatgcca ggttaccgat ctgcaaatta caaatgctgg cgtcatgctc
    27841 gctacaaatc aggatttacc cagcgagacg tttgatttag cggtgattgc cacgggtcac
    27901 gtctggcctg atgaagaaga agcaaccaga acgtattttc ccagtccgtg gtcaggcctg
    27961 atggaagcaa aggtcgatgc gtgtaacgtg ggtattatgg gaacatcctt gagcggactg
    28021 gatgcggcaa tggcagtggc tattcagcat ggttcgttca ttgaagatga taaacaacac
    28081 gtcgttttta accgcgataa cgcaagtgaa aagctaaata ttacgttgat gtcgcgcacg
    28141 ggtattttac ccgaagccga tttctattgc cctattccct acgagccctt acacatcgtc
    28201 accgatcagg cattaaatgc tgagattcaa aaaggtgaag agggcctttt ggatcgggta
    28261 tttagattga tagtagagga aatcaagttt gctgatccag actggagcca acgcatagcc
    28321 ttagagagcc tgaatgtcga ttcctttgct caagcctggt ttgccgagcg caaacaacgc
    28381 gacccatttg actgggcaga aaaaaatctc caggaagtcg aacgcaataa acgagaaaaa
    28441 catactgttc cctggcgtta tgtcattctg cgcctgcatg aagccgtaca ggaaattgtt
    28501 cctcatctga atgaacacga ccataaacgg ttcagtaaag gccttgccag ggttttcatc
    28561 gataattatg cggcaatccc ttcagagtct attcgtcgcc tacttgcctt acgtgaagcg
    28621 ggaatcattc atattctcgc cctcggtgaa gactacaaaa tggaaattaa tgagtcgcgc
    28681 accgtcctga aaacggaaga caacagctac tcgtttgacg tttttattga tgcccgcgga
    28741 cagcgtccgc ttaaagtgaa agatattcct ttccctgggc tacgcgagca attacagaaa
    28801 acaggggatg aaatccctga tattggcgaa gattatacgt tacagcaacc cgaagatatt
    28861 cgtggacgcg tagcgttcgg cgcgttgccc tggttgatgc acgaccagcc tttcgttcag
    28921 ggacttacgg catgtgcaga aattggtgag gcgatggctc gggcggtcgt gaagcctgca
    28981 tcccgtgctc gtcggcgtct ttcgtttgat taaaggtaaa gctatcagca ctcaacaaac
    29041 tgatgctgtt cattaagacg atagcggact ccggttcgaa tattgttttc accttcaatg
    29101 gcgacggcga agcgaactct ttcgccgtca tgatatgcca gtgcggcgct tccccctggc
    29161 ccgagaataa ttgagtcgac cacgcccgtg ctcactaccg ttgaattttc accgctggca
    29221 atgatgtgta cattgtcgcc gctgttggca attctggcgt ttgcgccaaa gctggcgatt
    29281 tgcaccaggt caccattact ggcgacatga caccgttcac ccaatgtaca aacgcgtact
    29341 cgcattccgg tattcgcaat ccgactacta tctccagcgc tgcttatccg cgaagaatta
    29401 ccggttacag caacccgtgc gcgttcaccg gagctgccaa tgtgactgtt aaagccgaca
    29461 cttccaatcc gcacgctata acccatactg gcaatttgcg cggcataccc agcggttgcg
    29521 attcgggcgt tatcctcaca acagccgatg cgagcatgat cgtcgctgcg ggcaaactga
    29581 ctgcaaaatg gtatctctcc ggggtctgtg gccagttttt gcatagagga aacttcctga
    29641 tgcgcgaagt tttcatccgc cagccatttt ttccacgcat actcaaccag actctccgcc
    29701 cagtccgtat atccttcctc aatcagtgca tgatgaatat cggcataact accgccgcgt
    29761 gggaaatggc gcaggaacca gcgatacatc accgctccga tacgccattc tcgcaggtct
    29821 gctcgtgtaa tgatcattcg ttagtgccgg tgatagccat cgaccatgct tttaaaggtt
    29881 tcgtgtggcg tacgccccgt ggtgcaaaga taaagatgac cgaagataaa aaagaggctt
    29941 ataaatgcca gagcaaaatg cgcctgcaat aaccagtatc ttacgccagg aaacacatct
    30001 cccacggctt gcggatagag acacagcagc cccgtcaata gtaacaacgg cagcaatcca
    30061 tacataacac caacataggc gacctgctgt aagggattaa atttagactg ggttgttgcc
    30121 gggaaaggat gttcttcccc ctgcataatg ccaaacaaat aaaatcgcgt ttgttttgcc
    30181 gctcgctcca gccacccctg acgacgaatg cgatagtggt gaccattacc cccaacggca
    30241 ttgatcagca caaagccgag ccagcatgcg agtaacaaaa atccgcaaac ttcatgcacc
    30301 gcaaccagac ttttaaccgc agttgcgccc accagcgcaa agtgatttat cagcccactt
    30361 gccagcaaca atacaaagag taacgcattc gaccagtgcc ataatctgac cgctttgcag
    30421 taaagataaa tcttctcacc ataatcggtg actgatttct tcacgccacg agcacgaagc
    30481 aaggcatgca gccccagcac caaccacatc ccaaccagca atactccggc aatgatcaac
    30541 cacaccggcc aaaattcggg agtgaactgt ggcacatagt tcgctaactg gctctgaaac
    30601 tgttcagcat gttgcgacgg gttcatacat tttccttttt aatcaaatgt tgaccgaacc
    30661 ggcgatacag atgcggtttc ccggcaccag gtagctgata ttgatagtat ttattttgct
    30721 gcaaccacgc ctgaatttct gggctatctt cgcgaccaaa gatcaatgcg tgttccgggc
    30781 aggcgctgac gcaaatgggc ggaaagcctt ttgccagtcg ggactcagcg cagaaatcac
    30841 atttatctgc cactttagtg acagggttaa ggtagcgaac ctggtacgga caagcgccaa
    30901 tgcaataact gcagccgatg cactgcgatt tttctacccg cacgatcccc tgctcatcac
    30961 gccacgacgc ccctgtcggg catacgtcga tgcacggagc atcttcgcag tgctgacatg
    31021 actggcgaaa aaagtgatac tgcgtttcgt tgtcgttatc ggtgactgga atgtgtgcta
    31081 tcgataaacg gcttccctga gcggggacgt ggttagtttt acggcatgcg cgggcgcaaa
    31141 tattacagcc attgcaccgt gactcgtcat gaatcatggc ataccgaact tccttttctt
    31201 gcctcgtgtt cgctaacaga gaagacgcag aaccagtaaa aaagattact gttcccatcc
    31261 ccagaacaaa tttgcgtcga gtgaaggaca tctgattatc ccctaatgca gtggctgcaa
    31321 ctgtccaccc tatccatgac atctccctga aatttcaggg gttctgaaac tgctctacct
    31381 cgaaccattc gcataagcca taagccttgc cataggtcat aaatcgctcg ctaaacaagt
    31441 acaacaccgc cgtcgatgac tccacctgcc gtatatcggc atattcaggt gcaacatcca
    31501 tcgcggcgat cgcggcttca atctgcgctt cctggaagta atatggcgct tgcatcagca
    31561 tggcgacttt gtaaggacga ggatatgtct ggcactcaaa acgaacggcg tgtgcaatgg
    31621 cgcgacaaat gtcctgctcc acaacttgca aagacatcgc cgcgtagttt tcactcatgg
    31681 cctgcgttga gtaatagtaa tcatcctggc ttcccgtcat acaagcgata tccgcgtagt
    31741 ctccgttctg cttaatgctt tccagcaaaa ctgacagctc ctcttctgca accgaatagg
    31801 gtggcgagag aaacacggcg cgcgccacca gctgcccagc cgcagaatgc tggcgaatga
    31861 actctgccag gatttcagcc ggttctggtt cggttaccgt atcaacgggg tgacgcatcc
    31921 cctgtaacca ttgccgcttc gcctcgtcaa cttcgctgac tttcgctaac ggcaattcat
    31981 cccgatggtt catctcaccc attccttaca caggcagtag attgtgtgct gccagatcgg
    32041 cggcaatatc ttccagcccc agacgctgca atgtttcgcg ggtcggacaa ccaagctgtg
    32101 ggtcccagcc catttctttg tagaacatcg tgagcgaagc atacatatcg tcacgatcca
    32161 ttttgtcagt accttcagta aagaccggga tctgcggatc cttgtcgaat acccaggaac
    32221 agataagatc gtgttcatta cgcatatctt tggtttgcat cagttttacc gtgtaggcac
    32281 gatgcaacgt aaagatacgc tctgcagcta aatctaattt ttcctgagtc atctcttcgc
    32341 cggtgatcgc tttgaagaat ttggcttcaa gcgccagatc gccccggtaa ttacggcttt
    32401 tcagcggcga aacggtcatt ggccagaccc agttgcacag ggtgacggcg ttatgcaaac
    32461 agacccgcaa cagcgaccac tttgcatatt taatttttgc gtcattgatt ggcgtgtagt
    32521 ttttggtttc atcgtaggca tcttcagaac caaaaagctc tttcgccact tcacgctgca
    32581 gtttcaatgg caaacccgaa ccaataaaat tgatatgggt atgcgtcatg cagtcacggt
    32641 tgaacataca gttaacaatg gaaccgacct gcgctgacgc ttcattggca tggtgaaccg
    32701 gatagccaaa cggcgaccag agtttatttt ttgcgtagcc ccagtactct tcacccaaat
    32761 tccagcgttc tgcgatggca tatgaaccat cagccaggtg actcagctca cccacacgat
    32821 gcgccagacg gtagtaaaaa tctttaatga aattaacgtc acccgcttcc agttgatccc
    32881 agtgaatttc cgcatactct tcagctggca gaacacgctt gaacacccct ttgctgtaac
    32941 aataagtaaa gtcgcgatgc agctgcccgt agttacacca taggccgtag tcatcgaaca
    33001 aattcagacc aaccaggtta ccaatcacac gaccatcgtc tttatcttca aaatctttcg
    33061 gcccgttcgg gaagatggtg gtatggacaa agttagctac acaagtgtta ccacctgtgc
    33121 tgggcacgcc aaactctttc acccgaggaa tattcatttg ggtcatacaa cggatcgggc
    33181 aagagtggca gccactcatt tttactgtgt atttctctgc cgccggacct aagtcaaaaa
    33241 cggatttata ggtacgaaag ccgaccgtgt tctgattgcc tggcggaatt tcccccgttt
    33301 caatcggacc accttcagcc gcgccccaaa acagcccttt acgtgctgtc caacgtgact
    33361 tgggatctga atactctgcc cacgattgcg gcgtacttgg cacgacatgg ttgttattcg
    33421 caccaataag ttcagtcatc atgtaatcgt tgagacgctt catctcctga cgatcggcaa
    33481 tgttgacccc tttcgtccct tcaaccgcaa tcgctttcag gtttttcgaa cccataattg
    33541 cgccagttcc cgcaccgccg ctgtggttac ggctattcaa catgccagag agaggaacaa
    33601 ggttttcccc agcctgacca atagccgcca cacaggtttc cggactggtc aatcgacaaa
    33661 tttcttccgt cgttgcgcgc gtcccttttc cccataagaa atcggctttt tccaggctaa
    33721 ctttgtcatc tttaatcttc agccataccg gtgatttcgc cttcccttcg ataataatga
    33781 cgtcgtatcc agcgaatttc atttgcgctg caaaaaagcc acccatatgg gcatcgacaa
    33841 ctaaatttcc tttggtaaaa gtagaaagtg aggtgatatt tacgcgagaa ctacaggggg
    33901 caccagatcc agttaatggg ccggtagcaa agactaattt attcgcttca tcgaaaggtt
    33961 tcgtgcctgg cggtacttcg tcatacataa ttttgtagcc gaagcccatg ccaccgacaa
    34021 aacttttaaa cttactggaa tcttcgaggg taatatttcc tgtcgtgaga ttgactctta
    34081 atatattacc tgtccaaccg ttagccatga ttttttcctt tgcaagattc acacagtaat
    34141 atctttccac tcgataattt ttaacgcccc tgttgggcag gcgtttgcgc attcaccgca
    34201 taacacacat ttcgaggatt ttttactttc ggtatttacg gtggccatca tccacgggca
    34261 tgccgtagta caggcgctac agccaataca gcgtttatga tcgacggtaa tacagccttc
    34321 tttctgctgc caggtaatcg caccaatcgg gcagacgttc atgcattgcg gctctttgca
    34381 ttgacggcag gtgtccgcgg tatagttgag atcgccatac aggccgccgc cagagccaac
    34441 cccgttgtcg ccaaagaaat aattgcggtg gattttgata cgggagaaga atgtccctac
    34501 tgagccatcg ttgaagttgg tacaagagat ttcacagcgg tggcaacccg tgcatcgcgc
    34561 tcgctgggtc accagcaccc ctttgggtgt atttatcaac cctactgtgc cgctatcaat
    34621 atcttcctgt ttgcaaccca aaagtgacag taacgcaggg gcaattgtta gaccagcaag
    34681 gccttttcct gatatacgca agaattctaa tcgcgttaag ccaatatcta atagtggacg
    34741 atcaaccggg ttcatttatt tcgcatcctc ttcgcaaaca cagtgctgac aattcagcag
    34801 cctgatatta agcattcaac taatacgctg ctgactctgt ccccaacacc agatattttc
    34861 cctaacccga tagtgtatga gaaaagattt aacgccgata aactaatcct ttatggttag
    34921 gagaaaaaat a
//
LOCUS       DAZFWL020000039        34135 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFWL020000039 DAZFWL020000000
VERSION     DAZFWL020000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.039"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(310..1376)
                     /locus_tag="ACHKNP_RS10060"
                     /old_locus_tag="pgaptmp_002012"
                     /pseudo
     CDS             complement(310..1376)
                     /locus_tag="ACHKNP_RS10060"
                     /old_locus_tag="pgaptmp_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(1379..1741)
                     /locus_tag="ACHKNP_RS10065"
                     /old_locus_tag="pgaptmp_002013"
     CDS             complement(1379..1741)
                     /locus_tag="ACHKNP_RS10065"
                     /old_locus_tag="pgaptmp_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU4704182.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2278..4191
                     /gene="mtlA"
                     /locus_tag="ACHKNP_RS10070"
                     /old_locus_tag="pgaptmp_002014"
     CDS             2278..4191
                     /gene="mtlA"
                     /locus_tag="ACHKNP_RS10070"
                     /old_locus_tag="pgaptmp_002014"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HGU4704183.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSTILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGEYIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4421..5569
                     /gene="mtlD"
                     /locus_tag="ACHKNP_RS10075"
                     /old_locus_tag="pgaptmp_002015"
     CDS             4421..5569
                     /gene="mtlD"
                     /locus_tag="ACHKNP_RS10075"
                     /old_locus_tag="pgaptmp_002015"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU4704184.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5569..6156
                     /gene="mtlR"
                     /locus_tag="ACHKNP_RS10080"
                     /old_locus_tag="pgaptmp_002016"
     CDS             5569..6156
                     /gene="mtlR"
                     /locus_tag="ACHKNP_RS10080"
                     /old_locus_tag="pgaptmp_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HGU4704185.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6168..6377)
                     /gene="yibT"
                     /locus_tag="ACHKNP_RS10085"
                     /old_locus_tag="pgaptmp_002017"
     CDS             complement(6168..6377)
                     /gene="yibT"
                     /locus_tag="ACHKNP_RS10085"
                     /old_locus_tag="pgaptmp_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HGU4704186.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6662..7024
                     /locus_tag="ACHKNP_RS10090"
                     /old_locus_tag="pgaptmp_002018"
     CDS             6662..7024
                     /locus_tag="ACHKNP_RS10090"
                     /old_locus_tag="pgaptmp_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HGU4704187.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            7567..8295
                     /locus_tag="ACHKNP_RS10095"
                     /old_locus_tag="pgaptmp_002019"
     CDS             7567..8295
                     /locus_tag="ACHKNP_RS10095"
                     /old_locus_tag="pgaptmp_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU4704188.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKKINAVSTLITRNSEHH"
     gene            8295..13145
                     /gene="ehaG"
                     /locus_tag="ACHKNP_RS10100"
                     /old_locus_tag="pgaptmp_002020"
     CDS             8295..13145
                     /gene="ehaG"
                     /locus_tag="ACHKNP_RS10100"
                     /old_locus_tag="pgaptmp_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HGU4704189.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKTTNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITASTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TSGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            13514..15169
                     /gene="lldP"
                     /locus_tag="ACHKNP_RS10105"
                     /old_locus_tag="pgaptmp_002021"
     CDS             13514..15169
                     /gene="lldP"
                     /locus_tag="ACHKNP_RS10105"
                     /old_locus_tag="pgaptmp_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HGU4704190.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            15169..15945
                     /gene="lldR"
                     /locus_tag="ACHKNP_RS10110"
                     /old_locus_tag="pgaptmp_002022"
     CDS             15169..15945
                     /gene="lldR"
                     /locus_tag="ACHKNP_RS10110"
                     /old_locus_tag="pgaptmp_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HGU4704191.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            15942..17132
                     /gene="lldD"
                     /locus_tag="ACHKNP_RS10115"
                     /old_locus_tag="pgaptmp_002023"
     CDS             15942..17132
                     /gene="lldD"
                     /locus_tag="ACHKNP_RS10115"
                     /old_locus_tag="pgaptmp_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HGU4704192.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            17318..17791
                     /gene="trmL"
                     /locus_tag="ACHKNP_RS10120"
                     /old_locus_tag="pgaptmp_002024"
     CDS             17318..17791
                     /gene="trmL"
                     /locus_tag="ACHKNP_RS10120"
                     /old_locus_tag="pgaptmp_002024"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HGU4704193.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(17844..18665)
                     /gene="cysE"
                     /locus_tag="ACHKNP_RS10125"
                     /old_locus_tag="pgaptmp_002025"
     CDS             complement(17844..18665)
                     /gene="cysE"
                     /locus_tag="ACHKNP_RS10125"
                     /old_locus_tag="pgaptmp_002025"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGU4704194.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(18745..19764)
                     /gene="gpsA"
                     /locus_tag="ACHKNP_RS10130"
                     /old_locus_tag="pgaptmp_002026"
     CDS             complement(18745..19764)
                     /gene="gpsA"
                     /locus_tag="ACHKNP_RS10130"
                     /old_locus_tag="pgaptmp_002026"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGU4704195.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(19764..20231)
                     /gene="secB"
                     /locus_tag="ACHKNP_RS10135"
                     /old_locus_tag="pgaptmp_002027"
     CDS             complement(19764..20231)
                     /gene="secB"
                     /locus_tag="ACHKNP_RS10135"
                     /old_locus_tag="pgaptmp_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGU4704196.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(20294..20545)
                     /gene="grxC"
                     /locus_tag="ACHKNP_RS10140"
                     /old_locus_tag="pgaptmp_002028"
     CDS             complement(20294..20545)
                     /gene="grxC"
                     /locus_tag="ACHKNP_RS10140"
                     /old_locus_tag="pgaptmp_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HGU4704197.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(20687..21118)
                     /locus_tag="ACHKNP_RS10145"
                     /old_locus_tag="pgaptmp_002029"
     CDS             complement(20687..21118)
                     /locus_tag="ACHKNP_RS10145"
                     /old_locus_tag="pgaptmp_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGU4704198.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            21363..22907
                     /gene="gpmM"
                     /locus_tag="ACHKNP_RS10150"
                     /old_locus_tag="pgaptmp_002030"
                     /gene_synonym="pgmI"
     CDS             21363..22907
                     /gene="gpmM"
                     /locus_tag="ACHKNP_RS10150"
                     /old_locus_tag="pgaptmp_002030"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HGU4704199.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            22941..24200
                     /gene="envC"
                     /locus_tag="ACHKNP_RS10155"
                     /old_locus_tag="pgaptmp_002031"
     CDS             22941..24200
                     /gene="envC"
                     /locus_tag="ACHKNP_RS10155"
                     /old_locus_tag="pgaptmp_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HGU4704200.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            24204..25163
                     /locus_tag="ACHKNP_RS10160"
                     /old_locus_tag="pgaptmp_002032"
     CDS             24204..25163
                     /locus_tag="ACHKNP_RS10160"
                     /old_locus_tag="pgaptmp_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HGU4704201.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMRALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(25150..26184)
                     /gene="waaH"
                     /locus_tag="ACHKNP_RS10165"
                     /old_locus_tag="pgaptmp_002033"
     CDS             complement(25150..26184)
                     /gene="waaH"
                     /locus_tag="ACHKNP_RS10165"
                     /old_locus_tag="pgaptmp_002033"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HGU4704202.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(26435..27460)
                     /gene="tdh"
                     /locus_tag="ACHKNP_RS10170"
                     /old_locus_tag="pgaptmp_002034"
     CDS             complement(26435..27460)
                     /gene="tdh"
                     /locus_tag="ACHKNP_RS10170"
                     /old_locus_tag="pgaptmp_002034"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HGU4704203.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(27470..28666)
                     /gene="kbl"
                     /locus_tag="ACHKNP_RS10175"
                     /old_locus_tag="pgaptmp_002035"
     CDS             complement(27470..28666)
                     /gene="kbl"
                     /locus_tag="ACHKNP_RS10175"
                     /old_locus_tag="pgaptmp_002035"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HGU4704204.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(28941..29798)
                     /gene="yibB"
                     /locus_tag="ACHKNP_RS10180"
                     /old_locus_tag="pgaptmp_002036"
     CDS             complement(28941..29798)
                     /gene="yibB"
                     /locus_tag="ACHKNP_RS10180"
                     /old_locus_tag="pgaptmp_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HGU4704205.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            30102..31034
                     /gene="rfaD"
                     /locus_tag="ACHKNP_RS10185"
                     /old_locus_tag="pgaptmp_002037"
     CDS             30102..31034
                     /gene="rfaD"
                     /locus_tag="ACHKNP_RS10185"
                     /old_locus_tag="pgaptmp_002037"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HGU4704206.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            31044..32090
                     /gene="rfaF"
                     /locus_tag="ACHKNP_RS10190"
                     /old_locus_tag="pgaptmp_002038"
     CDS             31044..32090
                     /gene="rfaF"
                     /locus_tag="ACHKNP_RS10190"
                     /old_locus_tag="pgaptmp_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HGU4704207.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            32094..33086
                     /gene="rfaC"
                     /locus_tag="ACHKNP_RS10195"
                     /old_locus_tag="pgaptmp_002039"
     CDS             32094..33086
                     /gene="rfaC"
                     /locus_tag="ACHKNP_RS10195"
                     /old_locus_tag="pgaptmp_002039"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HGU4704208.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            33083..>34135
                     /gene="rfaL"
                     /locus_tag="ACHKNP_RS10200"
                     /old_locus_tag="pgaptmp_002040"
     CDS             33083..>34135
                     /gene="rfaL"
                     /locus_tag="ACHKNP_RS10200"
                     /old_locus_tag="pgaptmp_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGU4704209.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKN"
BASE COUNT         8783 a         8589 c         8533 g         8230 t
ORIGIN      
        1 tattttttaa atgtgactgt catccttcag ataataaagc catcatcgca atagatggaa
       61 aagcactcct gctctttttt gattagagcc gtcgcaggag gaacaactca tatcatcatt
      121 gcgatctcga cgatgtatag tctcatcctg cggcagaaca agacggataa aaaatccaat
      181 gagagcacac tcatccataa cttattaaca taatgaatat taaagaaaaa tcatcgcagc
      241 taatgaagta atatgcaaaa ataattagtc cttacaagga gcaaagttcc ataatctccc
      301 ccctccccct caatgatcca aataaagata atgcatccag ctggtcattc ttagcagcac
      361 tttccgcatc accgaaacat ggctgaaatg gtcggagtaa accgccacct gggcgtagat
      421 gccgtcgggt aaggcatcga tatcatcgtt agggtccagt tcaatggttc ccagcacacc
      481 gtccgtgccg ggcacgaccg ttaatgattg caataccccc tgcgcctgat aagaaccgcc
      541 tggcacgaca ggtaaaatac tggtcagttt gccgtggaac acctgcccag gtagcgcgtt
      601 aaacaccact tctgcatcat caccaggttt cagacgtaac agcgagtttt gccgaaattg
      661 ggcgacaatt tgccgttttt gctcggggat gaaaaccatc accggacgca acggcaaggc
      721 agctgcgtat gtacctgggc ggatcagtac ctgagtaatg tagccattgc tcggcgcgcg
      781 aatgacagtc tgctcaagat tatattttgc ttcagtaagt tgcgctctta agctcacaat
      841 ctgcgattgc tcgccgttaa ccatactgtc gagctggctc tggatctgcg cctgctccgc
      901 caccgagcct ttcaccagcg catcctgcgc gaggaaattt tgccgcgcat cgtcgatgtc
      961 acgttccgag aacggattca ccgccgcctg gctgcctttc agataacgtt gataattttt
     1021 aaacagacgg tcgcgctccg ctgaaacctg ggtggtgttg gcctgcgctt ctgtgagctg
     1081 cgcgcgcaga gtctttatat tatgcgtcgc cgtcatcagg tcagcctgaa gcctgtcaac
     1141 tcgcgcctgg taacgaaccg ggtcgagctt aaaaagcacc tcgccctttt gaataagctg
     1201 attattcttg tcagtgactt cagtaacaat tcccgtcacc tgtggcgtga tggggatcgc
     1261 tatcactgcc ttttgcgcgg taaaagtgta agggtggttg tagttcatca acaaaatcaa
     1321 accactcacc agaaacacgc cagcgccacg taagttaaaa caatcaatag atccataatt
     1381 agatctccgg gaacgtggaa tagtcagtct ttttctcggc ggcgagttga tgctccagcc
     1441 gggaaattcg gtcagaaagt gcggcgattt ccgggtcagt cgctttctcc tgcgacgcaa
     1501 catgtgactg cataccccag ccacgctccg gttgatagag cgtcgcccag atccacagaa
     1561 acggccagat aacatgcaga gtaaacaggc tcacccagcc cgccgtatga atagcgtcgg
     1621 catggggatg gttgcgcttt ttggcaatca gataagggat gtcatgtatc gcgatgattc
     1681 cataaaaaat caccagaaag acgaagatca gcactcccaa cgcgaaatag tttagaaaca
     1741 tatctgcctc ggattcacgt ttatcagtgt tgtttttggg ctggcagcca gaagggagtc
     1801 aggctgatat tttgacaata atccgggttc gcgattctcg ccataacacc aaagaataat
     1861 ttttagaggt gatgagttgc ttagttacat aacgattgta tgacgaaggc ataacatgct
     1921 gtagatcaca tcaggtgaac gccgtaagaa aatatcttgt gattcagatc acaaagattc
     1981 aacaaaccat caaaacaaaa atgtgacact actcacattt aaatgccatt tttagcgaaa
     2041 atcgccgcct tgttgctttt ttacacaagc gttttgtgat gaacgtcacg tcaattacct
     2101 ctctaccccc tatatttatg tgattgatat cacacaaaag gccgtcgact ggacagttaa
     2161 ccgattcagt gccagatttc gcagtatcta caaggtccgg ctacctctgc cgccacatta
     2221 acaaaaaacc tcgggcttcc agcctgcgcg acagcaaaca taagaagggg tgtttttatg
     2281 tcatccgata ttaagatcaa agtgcaaagc tttggtcgtt tcctcagcaa catggtgatg
     2341 ccaaatatcg gcgcgtttat cgcgtggggt atcatcaccg cgttatttat tccaacaggg
     2401 tggttaccga acgagacgct ggcgaagctg gtcgggccga tgatcactta tctcctgccg
     2461 ctgctgatcg gttataccgg tggtaagctg gtaggcggcg aacgtggcgg cgtagtcggt
     2521 gccatcacca ccatgggcgt tatcgtcggc gcagacatgc cgatgttcct cggttctatg
     2581 attgcaggtc cgctgggcgg ctggtgcatt aagcacttcg accgctgggt agacggtaag
     2641 atcaaatccg gttttgagat gctggtgaat aacttctccg caggcatcat cgggatgatc
     2701 ctcgctattc tggcattcct cggcattggc ccgattgttg aagccctgtc caaaatgctg
     2761 gctgcgggcg ttaacttcat ggttgtccat gacatgctgc cgctggcgtc tatctttgtt
     2821 gaaccggcga aaatcctgtt cctcaacaat gccattaacc acggtatctt ctcgccgctg
     2881 ggtattcagc agtcccatga actgggtaaa tcaatcttct tcctgattga agctaaccca
     2941 ggtccaggta tgggcgtgct gctggcgtac atgttctttg gtcgtggtag cgctaaacag
     3001 tctgcgggcg gtgcggcaat catccacttc ctgggtggta tccacgaaat ctacttcccg
     3061 tatgtgctga tgaatccgcg tctgatcctc gcagtcatcc tcggcggtat gactggcgtg
     3121 ttcacgctga ctatcctggg cggtggtctg gtttctccgg catctccggg ttctatcctt
     3181 gctgtactgg cgatgacacc aaaaggtgct tatttcgcta acatcgcggg tgtgtgtgcg
     3241 gcgatggctg tctccttcgt tgtctctact attttgctga aaaccagcaa agtgaaagaa
     3301 gaagatgata ttgaagcagc aactcgtcgt atgcaggaca tgaaagctga gtctaaaggc
     3361 acatctccgc tgtctgcggg cgatgtgact aacgacctga gccacgtacg taaaatcatc
     3421 gttgcctgtg acgccggtat gggttcaagt gcgatgggcg caggcgtgct gcgtaagaaa
     3481 attcaggatg caggtctgtc acagatttcg gtcactaaca gcgcgatcaa caacctgccg
     3541 ccagatgtgg acctcgtcat cactcaccgt gacctgaccg aacgcgctat gcgccaggtt
     3601 ccgcaggcgc agcatatttc gctgaccaac ttcctcgaca gcggcctgta caccagccta
     3661 accgaacgtc tcgttgctgc ccagcgccat actgaaaacg aagtgaaagt gaaagacagt
     3721 ctgaaagaca gctttgacga ttccagtgct aacctgttca aactgggcgc ggagaacatc
     3781 ttcctcggtc gcaaagcagc aaccaaagaa gaagcgattc gttttgctgg cgagcagctg
     3841 gtgaaaggcg gttacgttga gccggaatac gttcaggcga tgctggatcg tgaaaaactg
     3901 accccgactt atctgggtga gtatatcgcg gtgccacacg gtacggttga agcgaaagat
     3961 cgcgtactga aaacgggcgt ggtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa
     4021 gaagaagatg acattgcccg tctggtgatt ggtattgcag cccgtaacaa cgagcacatt
     4081 caggttatca ccagcctgac caatgcactg gatgatgagt ccgtcatcga gcgtctggca
     4141 cacaccacca gcgtggatga agtgctggaa ctgctggcag gtcgtaagta atccaatccc
     4201 accctctcca catggagaag gtggggttaa ttgcctgatg cgctacgctt atcaggccta
     4261 caggatgcat cacaatttgt tgaatttgca cgttcttgta ggccggataa ggcgcttacg
     4321 ccgcatccgg cgctgcccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc
     4381 ccagccctct cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg
     4441 cgcaggtaat atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact
     4501 gacgtttgcc gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca
     4561 ggttcatgtg gttggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag
     4621 cagcattggt gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt
     4681 tggcccggtt gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa
     4741 agaacaaggt aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggtac
     4801 cacgcagctg aaaggccatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga
     4861 agaacacgtt ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc
     4921 aactaacgat ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac
     4981 gcagttcaaa ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc
     5041 atttgtcgaa cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg
     5101 aaaactggcc ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt
     5161 ggtaaaaggt gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc
     5221 agacaaacat gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa
     5281 agatgatgta gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct
     5341 gatcaagcca ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg
     5401 gattgctggt gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc
     5461 actgatcgct gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa
     5521 cagcgaggtt gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag
     5581 gcgcaggaca ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa
     5641 acccaggcct ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc
     5701 tttctgatca ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta
     5761 ttccgcaaag acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt
     5821 ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc
     5881 caggaatacg aagatgcgga gctgctgatg gcattgcgtg aagaactaaa tcacgacggc
     5941 aacgagtacg cctttaccga cgacgagatc cttggaccct ttggtgaact gcactgcgtg
     6001 gcggcgttac caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa
     6061 attcagcgct atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt
     6121 tccagaatca gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct
     6181 acctgcttcg gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg
     6241 ctttcatcgg gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc
     6301 cggaacgcct ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt
     6361 tcacctaatt tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc
     6421 caattttagt ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat
     6481 aatcatccgc tgctgtttat tatctttttt ctatagattt cttaattaat caacgaattg
     6541 cgtagttttt gagcatgtta atgattacgt tgcagaacta ttacaatgtc atcagttgtg
     6601 aacaaagcac ctggtcgcgc atactagggg ctataaattt atctttatca gaagccatca
     6661 catgaaagaa gtcgaaaaaa acgaaatcaa acgtctcagc gatcgcctgg acgccatccg
     6721 ccaccagcag gccgatctgt cgctggttga agccgcagac aaatatgccg agctggaaaa
     6781 agagaaagcc acgctggaag cagaaattgc tcgcctgcgt gaggttcata gccaaaaact
     6841 gagtaaagaa gcacagaagc tgatgaagat gccgtttcag cgcgcaatta ccaaaaaaga
     6901 gcaggctgat atgggcaagc tgaagaaaag tgttcgcgga ctggtggttg tgcacccaat
     6961 gaccgcgctg ggccgtgaaa tgggcctgca ggagatgact ggattctcta agactgcgtt
     7021 ttaagcgcct cagacgactg gttcgatcat atcaacccgc atctggcttg ccttagctgg
     7081 tcaatgcggg gactctcctg tctactcgcc taattaccaa acaattcact ccctttacgt
     7141 cgatttaccg ctggttgata ttctgaaaat gcatttttac cgaaatagcc acctataaac
     7201 aataaaaata tatagaagat atcatttatt aattaacaag catttacgtc tctttttttc
     7261 ggatttaatt gtgaaatttt aatcatcata ttcatacaat aaattatctg taaatttttt
     7321 cccagacatg gtttctttca tatttgacat ttattaataa tcatcattag aatccacgaa
     7381 aataatatat tcatacaaac acagacatct tttgaaacga atgatacatt tttatgatga
     7441 atgcttaaaa caatatttaa tcgacacaca tcactgtcat taatgtaaag caacatagat
     7501 caccaataat acaatggaca actctaccgg cattgatcgc tggtcggagg aaataaggat
     7561 ttttacatga acttgaagaa gatttttttc agtgctgtca ctgtttcagt tctctgcgca
     7621 ttaacgggat gtgactacat tgaagaaggc aagccagaaa gtagtttatt gaaacaacaa
     7681 gaagaacata acaataaaat tgatctactt gaaaaacaac aagcacagct aaagagtcaa
     7741 ctggaaacta ttcaaaaaca acaaactggc ataataagca gtacaaagac tttaactcac
     7801 gtaattaaat ccgttaaaga tcaacaaaac acttttattt ttaccgagtt taacccagca
     7861 aaaacgaagt atttcatcct gaacaatggt tcggtggctt tagcgggtcg agtgttatcc
     7921 attgacgcca cagagaatgg cagtgttatt catatttcac tggtcaactt attaagtaca
     7981 cctatctcaa atattggttt taatgcgaca tggggtggtg aaaaacctgt cgatgccaaa
     8041 gagtttgccc ggtggcaaca attacttttc aacacatcaa tgaaatccac attgaaatta
     8101 ttaccaggtc aatggcaaga cattaatttg accctgaagg gtgtatcgcc caataacctg
     8161 ggatatctga agttagccat caacatggaa aatattcagt tcgacaatct tccttctgct
     8221 gaaaatcggc agaaaagaag caaaaaaata aatgcagtta gtacgcttat aacaaggaat
     8281 agcgagcatc attaatgaac aaaatattta aagttatctg gaaccctgcg acagggaatt
     8341 atactgttac cagcgaaacg gcaaaaagcc gtggcaagaa atctgggcgc agtaagctgt
     8401 taatttctgc gctggttgcg ggtggaatgt tgtcgtcgtt tggggcattg gcgaatgccg
     8461 ggaatgacaa cggtcagggt gttgattacg gtagtggatc agctggcgac ggctgggttg
     8521 ctataggcaa aggggcgaaa gcaaatactt ttatgaacac cagtggttcc agtactgctg
     8581 tgggttatga cgctatagct gaaggccaat atagctctgc catcgggtca aaaacccatg
     8641 cgattggtgg tgcatcaatg gcctttgggg ttagtgcaat atcagaaggc gatagaagta
     8701 tagcactggg tgcctcttcg tattcattgg gccaatactc aatggccctc ggccgttatt
     8761 caaaagcatt gggtaaattg tctattgcta tgggggactc ttccaaagcg gaaggagcaa
     8821 acgccattgc cctgggaaat gccactaaag ctactgagat tatgagtatt gctcttggcg
     8881 acaccgccaa tgcgtcaaaa gcgtattcaa tggcgctggg agcaagtagc gtcgcatctg
     8941 aagaaaacgc aattgccctg gggcgtagca gtgtagctag cggtactgac agcctcgcat
     9001 ttggcagaca atcacttgcc agcgcagcga acgctattgc gataggtgct gagaccgaag
     9061 ccgctgaaaa tgcaactgct attggcaata atgcgaaggc aaaagggact aatagcatgg
     9121 caatggggtt cggaagcctt gccgataaag tcaatactat cgcattagga aatggcagcc
     9181 aggctctggc agataatgca atcgccatag gccagggcaa caaagctgat ggcgtggatg
     9241 ccatcgctct gggtaatggt agccagtcga gaggcttaaa caccattgcc ttaggcacag
     9301 ccagtaatgc aactggtgat aagagtcttg cgcttggtag taatagcagt gccaacggta
     9361 ttaactctgt cgcgctgggc gcagattcca ttgcggattt agacaatacc gtctctgtcg
     9421 gcaatagttc attaaaacgc aagatcgtta atgtgaaaaa tggcgcgatc aagtctgaca
     9481 gttacgatgc cattaatggt tcacagcttt atgccattag cgactcggta gcaaaaaggc
     9541 ttggaggagg ggctgcagta gatgttgatg acggtactgt tacagcacca acctacaatt
     9601 taaaaaatgg tagcaaaaat aacgtagggg ctgcgctcgc tgtacttgat gaaaacaccc
     9661 tgcaatggga ccaaaccaaa ggcaaataca gcgctgctca tggtactagt agcccaactg
     9721 ccagcgtaat caccgatgtt gcggatggca cgatttcagc ctccagtaag gatgcggtta
     9781 acggttccca actgaaaact accaatgacg atgtcgaagc caacaccgcc aatatcgcta
     9841 ctaataccag caacattgcc acgaatacgg caaatattgc caccaatacc accaatatca
     9901 ccaacctgac ggattccgtt ggtgaccttc aggctgatgc cctgctctgg aacgaaacta
     9961 aaaaggcatt cagtgcagct cacggccagg ataccaccag caaaatcacc aacgttaaag
    10021 atgccgacct gacggctgac agcactgatg ctgttaacgg ctctcagctg aaaaccacca
    10081 acgatgctgt ggcgacgaat accaccaata tcgccaataa cacttccaat attgccacta
    10141 acaccaccaa catctctaac ctgactgaga cggtgactaa tcttggtgag gatgcgctga
    10201 aatgggataa ggacaatggt gtattcacgg cagctcatgg caccgagacc accagcaaaa
    10261 tcaccaacgt taaagatggc gacctgacga ctggcagcac cgatgccgtt aacggctctc
    10321 agctgaaaac caccaacgat gccgtggcga cgaataccac caatatcgcc actaacacca
    10381 ccaacatctc taatctgact gagacggtga ctaatcttgg tgaggatgcg ctgaaatggg
    10441 ataaggacaa tggtgtcttc actgcagctc atggcaacaa taccgccagc aaaatcacca
    10501 atatcctgga cggcacagtc actgcaacca gttccgatgc cattaacggt agccagcttt
    10561 atgacttaag cagcaatatc gccacctact tcggcggcaa tgcttctgtg aatactgacg
    10621 gtgtgtttac cggtccaacc tacaaaatcg gtgaaacaaa ttattataac gtcggcgatg
    10681 cactggctgc gattaactcc tcatttagca cgtctctcgg cgatgctctg ctttgggatg
    10741 ccaccgcagg taaattcagt gccaaacacg gtactaatgg tgacgcaagc gtgatcactg
    10801 atgtcgcaga tggtgaaatt tcagactcca gttctgacgc agtaaacggc tcacaactcc
    10861 acggcgtgag cagttatgtt gttgatgcgc tggggggtgg tgccgaagtc aatgcagacg
    10921 gcaccatcac tgcgccgacg tacaccattg ctaatgctga ttacgataat gtcggtgatg
    10981 ccctgaatgc tatcgatacc actcttgacg acgctctgct ctgggatgcg gacgccggtg
    11041 aaaatggtgc atttagcgcc gctcacggaa aagataaaac tgccagtgta atcactaacg
    11101 tcgctaacgg tgcaatctct gctgccagca gcgacgcgat taacggctca caactctata
    11161 ccaccaataa gtacatcgct gatgcgctgg gtggtgacgc agaagtcaac gctgacggca
    11221 ccatcaccgc atcgacttac accattgcga acgccgagta caacaacgtc ggtgacgccc
    11281 tggatgcgct tgatgataac gccttgctgt gggatgagac tgccaatggc ggtgctggag
    11341 cctacaatgc cagccatgac ggtaaagcca gcatcatcac taatgtcgct aatggcagta
    11401 ttagtgagga cagtaccgat gcagtgaacg gttctcagtt gaatgcgacg aatatgatga
    11461 ttgagcagaa cacccaaatt atcaatcagc tcgctggtaa caccgacgca acctatatcc
    11521 aagaaaacgg tgcgggtatt aactatgtgc gtactaacga cgacggctta gcgttcaacg
    11581 acgccagcgc acagggtgtt ggcgctacag ctataggtta taactctgtc gccaaaggcg
    11641 atagcagcgt agctattggt cagggcagct acagcgacgt tgatacgggt atcgccctgg
    11701 gtagcagctc tgtttccagc cgagtgattg ccaaaggctc ccgtgacacc agcataacgg
    11761 aaaatggcgt tgttattggt tacgacacca cggatggcga actgctcggt gcattgtcta
    11821 tcggtgatga cggtaaatat cgtcaaatca tcaacgtagc cgatggttcc gaagcccatg
    11881 acgccgttac ggttcgtcaa ttgcagaatg cgattggtgc ggtcgcaacc acgccgacta
    11941 aatacttcca cgctaattca acggaagaag attcactggc agtgggaact gactcgctgg
    12001 caatgggtgc gaaaaccatc gtgaatggcg ataaaggtat tggtatcggt tatggtgcct
    12061 acgtggacgc gaatgcactt aacggcattg ccattggtag caatgcgcaa gtcattcatg
    12121 tcaacagtat tgcgataggt aatggttcta cgaccactcg tggcgctcaa accaattata
    12181 ccgcctacaa catggacgca ccgcagaact ctgtcggtga attctcagtc ggtagtgcgg
    12241 atggtcaacg tcagatcact aacgtcgcag caggttcggc tgataccgat gcggtcaacg
    12301 tgggtcagtt gaaagtaacg gatgcgcagg tttcccagaa tacccagagc attactaacc
    12361 tgaacaatca ggtcactaat ctggatactc gcgttactaa tatcgaaaac ggtattggcg
    12421 acattgtcac ctccggtagc accaagtact tcaagaccaa caccgatggc gtagatgcca
    12481 acgcccaggg taaagatagc gttgctattg gttctggttc cattgctgcc gctgacaaca
    12541 gcgtcgcact gggtaccggt tccgttgcag aggaagaaaa tacaatctct gtaggttctt
    12601 ctactaacca acgccgtatc accaacgtag ctgccggtgt taatgccacc gatgcggtta
    12661 acgtgtcgca gctgaaatct tctgaagcag gcggcgtgcg ctacgacacc aaagctgatg
    12721 gttctatcga ctatagcaat atcaccctcg gtggtggcaa cggcagtacg actcgtatca
    12781 gcaacgtctc cgctggcgtc aacaacaacg acgcggtgaa ctacgcgcag ttgaagcaaa
    12841 gcgtgcagga aacgaagcaa tacaccgatc agcggatggt tgagatggat aacaaactgt
    12901 ctaaaactga aagcaagttg agcggtggta tcgcttctgc aatggcaatg accggtctgc
    12961 cgcaggctta tacaccgggt gccagcatgg cttctattgg tggcggtact tacaacggtg
    13021 aatcggcagt tgctttaggt gtatcgatgg tgagcgccaa tggtcgttgg gtctacaaat
    13081 tacaaggtag taccaatagc cagggtgaat actccgccgc actcggtgcc ggtattcagt
    13141 ggtaatgatc cattaagtta gtgtgactaa gggcaaacgc caaattgcct gatgcgttac
    13201 gcgcatcagg caagataagg cgctccgggt caacaatcta agggcaattc tctgatgagg
    13261 attgcccttt tctttaccag acatctcccc cctacaagaa ttggccctac caattcttcg
    13321 cttatctgac ctctggttca caattcccca attaaaactc acatcaatat tgccaataca
    13381 taacatttag ttaaccattc attgtcatta tccctacaca acacaattgg cagtgccact
    13441 tttacacaac gtgtgacaag gagatgagca acagactcat tacacgatgt gcgtggactc
    13501 caggagacct gcaatgaatc tctggcaaca aaactacgat cccgccggga atatctggct
    13561 ttccagtctg atagcatcgc ttcccatcct gtttttcttc tttgcgctga ttaagctcaa
    13621 actgaaagga tacgtcgccg cctcatggac ggtagcaatc gcccttgccg tggctttgct
    13681 gttctataaa atgccggtcg ctaacgcgct ggcctcggtg gtttatggct tcttctatgg
    13741 tttgtggccc atcgcgtgga tcattattgc agcagtgttc gtctataaga tctcggtgaa
    13801 aaccgggcag tttgacatca ttcgctcgtc tattctttcg ataacccctg accagcgtct
    13861 gcaaatgctg atcgtcggtt tctgtttcgg cgctttcctt gaaggagccg caggctttgg
    13921 cgcaccggta gcaattaccg ccgcattgct ggtcggcctg ggttttaaac cgctgtacgc
    13981 cgccggactg tgcctgattg ttaacaccgc gccagtggca tttggcgcga tgggcattcc
    14041 aatcctggtt gccggacaag taacaggcat cgacagtttt gagattggcc agatggtggg
    14101 gcgtcagcta ccgtttatga ccattatcgt gctgttctgg atcatggcga ttatggacgg
    14161 ctggcgcggt atcaaagaga cgtggcctgc ggtcgtggtt gcgggcggct cgtttgccat
    14221 cgctcagtac cttagctcca acttcattgg gccggagctg ccggacatta tctcttcgct
    14281 ggtatcactg ctctgcctga cgctgttcct caaacgctgg caaccagttc gcgtattccg
    14341 cttcggtgac ttgggggcgt cacaggttga tatgacgctg gcccacaccg gttacaccgc
    14401 gggtcaggtg ttacgtgcct ggacaccgtt cctgttcctg accgctaccg taacactgtg
    14461 gagcatcccg ccgtttaaag ccctgttcgc atcgggtggt gcgctgtatg agtgggtgat
    14521 caacattccg gtgccgtacc tcgataaact ggttgcccgt atgccaccag tggtcagcga
    14581 ggctacagcc tatgccgccg tgtttaagtt tgactggttc tctgccaccg gcaccgccat
    14641 tctgtttgct gcactgctct cgattgtctg gctgaagatg aaaccgtctg acgctatcag
    14701 caccttcggc agcacgctga aagaactggc tctacccatc tactccatcg gcatggtgct
    14761 ggcgttcgcc tttatctcga actattccgg actgtcatca acactggcac tggcactggc
    14821 gcacaccggt catgcattca ccttcttctc gccgttcctc ggctggctgg gggtattcct
    14881 gaccgggtcg gatacctcat ctaacgccct gttcgccgcg ctgcaagcca ccgcagcaca
    14941 acaaattggc gtctctgatc tgttgctggt tgccgccaat accaccggtg gcgtcaccgg
    15001 taagatgatc tccccgcaat ctatcgctat cgcctgtgcg gcggtaggcc tggtgggcaa
    15061 agagtctgat ttgttccgct ttactgtcaa acacagcctg atcttcacct gtatggtggg
    15121 cgtgatcacc acgcttcagg cttatgtctt aacgtggatg attccttaat gattgtttta
    15181 cccagacgcc tgtcagacga ggttgccgat cgtgtgcggg cgctgattga tgaaaaaaac
    15241 ctggaagcgg gcatgaagtt gcccgctgag cgccaactgg cgatgcaact cggcgtatca
    15301 cgtaattcac tacgcgaggc gctagcaaaa ctggtgagcg aaggcgtgct gctcagtcga
    15361 cgcggcggcg ggacgtttat tcgctggcgt catgacacat ggtcggagca aaacatcgtc
    15421 cagccgctaa aaacgctgat ggccgatgat ccggattaca gtttcgatat tctggaagcc
    15481 cgctacgcca ttgaagccag caccgcatgg catgcggcaa tgcgcgccac acctggcgac
    15541 aaagaaaaga ttcagctttg ctttgaagca acgctaagtg aagacccgga tatcgcctca
    15601 caagcggacg ttcgttttca tctggcgatt gccgaagcct cacataacat cgtgctgctg
    15661 caaaccatgc gcggtttctt cgatgtcctg caatcctcag tgaagcatag ccgtcagcgg
    15721 atgtatctgg tgccaccggt tttttcacaa ctgaccgaac aacatcaggc tgtcattgac
    15781 gccatttttg ctggtgatgc cgacggggcg cgtaaagcaa tgatggcgca ccttagtttt
    15841 gttcacacca ccatgaaacg attcgatgaa gatcaggctc gccacgcacg gattacccgc
    15901 ctgcccggtg agcataatga gcattcgagg gagaaaaacg catgattatt tccgcagcca
    15961 gcgattatcg cgccgcagcg caacgcattc tgccgccgtt cctgttccac tatatggatg
    16021 gtggtgcata ttctgaatac acgctgcgcc gcaacgtgga agatttgtca gaagtggcac
    16081 tgcgccagcg tattctgaaa aacatgtccg acttaagcct ggaaacgacg ctgtttaatg
    16141 agaaattgtc gatgccggta gcactggcac cggtgggttt gtgtggcatg tatgcgcgtc
    16201 gtggcgaagt tcaggcagcc aaagcggcgg acgcgcatgg cattccgttt actctctcga
    16261 cggtttccgt ttgcccgatt gaagaagtcg cgccagccat caagcgccca atgtggttcc
    16321 agctttatgt actgcgcgat cgcggcttta tgcgtaacgc gctggagcga gcaaaagcag
    16381 cgggttgttc gacgctggtt ttcaccgtgg atatgccgac accgggcgca cgctaccgtg
    16441 atgcgcattc aggtatgagc ggcccaaatg cggcaatgcg ccgctacttg caggcggtga
    16501 cgcatccgca atgggcgtgg gatgtgggcc tgaacggtcg tccacatgat ttaggtaata
    16561 tctcagctta tctcggcaaa ccgaccggac tggaagatta catcggctgg ctggggaata
    16621 acttcgatcc gtccatctca tggaaagacc ttgagtggat ccgcgatttc tgggatggcc
    16681 cgatggtgat caaagggatc ctcgatccgg aagatgcgcg cgatgcagta cgttttggtg
    16741 ctgatggaat tgtggtttct aaccacggtg gccgccagct ggacggtgta ctctcttccg
    16801 ctcgtgcgtt gcccgctatt gcagatgcgg tgaaaggtga tatcgccatt ctggcggata
    16861 gcggaattcg taacgggctt gatgtcgtgc gtatgattgc gctcggtgcc gacaccgtac
    16921 tgctgggtcg tgctttcctg tatgcgctgg caacagcggg ccaggcgggt gtagctaacc
    16981 tgctaaatct gatcgaaaaa gagatgaaag tggcgatgac gctgactggc gcgaaatcga
    17041 tcagtgaaat tacgcaagat tcgctggtgc aggggctggg taaagagttg cctacggcac
    17101 tggctccgat ggcgaaaggg aatgcggcat agtcgtttgc gccccctcac cctaaccctc
    17161 tccctcaggg agaggggacc gttcggcgct gtatgtactc cctcactctg aaacgacacc
    17221 gcactctttt tttctccctc gcccctccgg ggtgagggga aaaggccgca ctgctcacca
    17281 attttctgct atcctgcccc cgcactaagg gggcagtatg ctaaacatcg tactttacga
    17341 accagaaatt ccgccaaata ctggcaatat catccgtctt tgcgctaata ccggctttcg
    17401 tctgcatatc atcgaaccga tgggatttgc ctgggacgat aagcgcctgc gccgcgcggg
    17461 gctggactat cacgagttta ccgccgttac gcgtcatcat gactttcgcg cgttcctcga
    17521 agcagaaaat ccccagcgcc tgtttgccct cactacgaaa ggtacgcctg ctcacagcgc
    17581 cgtaaactat caggatggcg attatctgat gttcggcccg gaaacacgcg gcctgccagc
    17641 gagcattctt gatgccctgc ccgctgaaca aaaaattcgc attccaatgg tgccggacag
    17701 ccgcagcatg aacctgtcca atgcggtgtc ggtagtggtg tatgaagcct ggcggcagct
    17761 gggatatccg ggagcgttgt tgagagatta gttactgtat gccggataga tgattacatc
    17821 gcatccggca cgatcacagc acatcagatc ccatccccat actcaaatgt atggttaata
    17881 ccgttgaaat gctggtccat atccattgat ggcttatcgc tgtctggttt accgacaata
    17941 cgagccggaa caccagcggc ggtagtatgc ggcggcaccg gttgcagcac cacggaacct
    18001 gcgccaatct tcgcgccgcg accaacttca atattgccga gaattttcgc gcccgcgcca
    18061 atcatcacac cttcacgaat tttcgggtga cggtcaccac cagatttacc cgtaccgcca
    18121 agcgtcacag attgcagaat cgatacgtcg ttttcaatta ccgccgtttc accaacgacg
    18181 atgcccgtgg cgtggtcgag catgataccg cgaccaattt ttgctgccgg gtgaatatcg
    18241 acctggaacg tcacagaaac ctggttttgc agaaagattg ccagtgcgcg acgcccctga
    18301 ttccacaacc agtgaccgat gcgataggcc tgcaaggcat gaaaaccctt caggtataac
    18361 aacggggttg agtatttatc gactgccggg tcgcgggtac gcaccgcctg aatatcacag
    18421 gccgcagagg cgatcatttc cgggtcagcg gcgtaggctt cttcaaccac ttcacggata
    18481 gcaatagcag gcataattgg cgatgacagc ttgttcgcca gcatgtagct cagggcactg
    18541 ccaaggtttt cgtgcttgag tagcgtcgcg tggtaaaaac tggccagcat tggctcacag
    18601 tccgccagcg ttctggcttc ggctttaata ttgttccaga caatttccag ttcttcacac
    18661 gacattgctt actccacacg atgagataat gaccggcccg ttctgcgcgg gccgggtcat
    18721 agcggtaaca aaggttccct ggggttagtg gctgctgcgc tcgtccttgc gtgcacgacc
    18781 tagtaaagtc aatgctgcct cgcgcgcgtt ttttccgcaa tataatactt gataaatttc
    18841 ctcggttatt ggcatttcaa cgccgaagcg atgcgccagt tcgcggactt ctttcgtatt
    18901 gcggtagcct tccaccacct gaccaatctt ctcctgcgcg ctttgtacat ccatgccctg
    18961 accgagcatc atgccaaaac ggcggttacg cgactggttg tcggtacagg taagcaccag
    19021 atcgcccagc cccgccatgc ccataaaggt ggcaggatcg gcacccagcg ccgcaccaag
    19081 acgcgacatt tcagccagcc cacgggtgat cagcgccgta cgcgcattcg caccaaaacc
    19141 gataccgtca gacatccccg cgccaatggc aatgacgttt ttcaccgcgc cgccaagctg
    19201 cacgccaatg aaatccgggt tgctgtaaac gcggaaactt ttgccacagt gcagcaattg
    19261 ttggagatca tcggcaaagg tctggtcggt agaggccaac gaaatagccg tcggtaaacc
    19321 tgccgccagt tctttcgcaa acgttgggcc agagataacc gccagcggaa tttgatcgcc
    19381 taaggcctca cgcgccacgt cctgtaacag acgtccggtt tccgcttcca gccctttggt
    19441 cgcccacacc agacgcgcat caggacgcat cagcggttta atctggcgca gcacttcacc
    19501 aaagacatgg ctgggtacga cgacgagaat attacggctg gctgccagcg cagtggcgag
    19561 atcgctttca agatggagcg tatcgggaaa aggcacatcg gggagaaacg cggcgttaca
    19621 gcggtcgcgt tcaagcgttg cgatatgttc agggtcatgg ccccagagga caacctcgtg
    19681 gccatttctt gccagggtaa tggcaagagc ggtgccgtac gagccggcac cgatcacagt
    19741 cattgaagca ttacgttggt tcatcaggca tcctgatgtt cttcagtacc ttcgccagcc
    19801 tgctgctgca aatagttcat gaacagcgca tcgaagttaa ccggcgcaag gttcagttgc
    19861 gggaatgtac cgcgggatac catgctggtg atgcactcac gagcatacgg gaacagaatg
    19921 ttcgggcagt atgcgcccag gcaatgtgcc atctgggtgc cttcgatacc cgcgatggag
    19981 aaaataccgc cctgctgaac ttcacacagg aacgcggttt cttcgcccag ggaggccgtt
    20041 acggtaacac gcagtaccac ttcgtatacg tcatctgcca gttgggaaga tgccgtatcc
    20101 agatcaagtt taacttctgg ttgccaatct ttctggaaaa cgtgcggcgc gttcggcgct
    20161 tcgaaagaga tatccttggt ataaatacgt tggatctgga aagtcatttc agtgttgttt
    20221 tgttctgaca tgtgtagaaa acccttaagt gttgtcctta aatactgcgc aatgtaatgc
    20281 caacgttcac acgttatttc agcaggggat ccagtccacc acgtgcatcc aatgcataca
    20341 agtcatcaca gccgccaatg tgctgtgcgt caataaaaat ctggggaacc gtggtgcgac
    20401 cgctgcgttt gatcatctct tcacgcttgg cggcattgcc atcgatcggc aactcctgaa
    20461 aactcacgcc cttgctgctc agcagtgctt ttgcacgatg gcaatacggg caggtttctt
    20521 tggtatagat ttcaacattg gccataattt agctcctgtt tttttaccct gaaatttcat
    20581 gttgcagggc ggctgcaaaa tggagtgtcc ccagaagctt ataacaataa gtcgctgggg
    20641 gcctaattgc agctaacggc ctgcatcatg aaagacgaca ggtaaattat ttgccgcgca
    20701 ccagaggcaa gttttcgcca gcccagccag caacgccctc tttcagcacg aatacttgcg
    20761 caaaaccagc tttcgtcagc gcgtttgcag gctcctggca ctgcatgcca gaaccgtcta
    20821 ccacgataac cggtttgtct ttgtgcttct caagctcacc aacattgttg gctttgattt
    20881 cactcggcaa cagattgata gaacctgcga tatggccttt acggaagtca tcacgctgac
    20941 gtaaatccac aaccacagcg tcttctttgt tgatcagacg cgtagcttca ccacgagtaa
    21001 tcaccttcac tttcgaggtc aggctcttaa acgtagtcac aagaaccgcc accagtaacg
    21061 cgatccaggc gatactcagt atgggatgac ggccaacaaa ttgcataatt tcttgcatgg
    21121 ggggtaacaa ctcccgacgt agtgattaaa aaaaccagga aaggagtata cctgcgcggt
    21181 gcggcaaata cagccagcgc gttaactgga atgcaatttt gcggggcacg acgaaaaaaa
    21241 agcgcaaatc tggcatcggc ttgccgcgag cggtcgtata ttttgatctt cagaggctat
    21301 tttatcgatt cagctgtagt aaaattacgc aaattttgac tcttgagtat gaggttgtcg
    21361 caatgtcggt ttctaaaaaa cctatggtac tggtgattct ggatggctat ggctatcgtg
    21421 aagaacagca ggataacgcc atttttagtg ctaaaacccc ggtaatggat gcactgtggg
    21481 ccaatcgtcc gcataccctg atcgacgctt ccggtctgga agtcggtctg cctgaccgtc
    21541 agatgggtaa ctccgaagta ggccacgtta acctgggtgc cggccgcatc gtgtatcagg
    21601 acctgactcg tctggacgtt gaaatcaaag atcgcgcttt ctttgctaat ccagtgctga
    21661 ctggcgcggt agataaagcg aaaaacgcag gtaaagcagt acacattatg ggtctgctct
    21721 ccgcaggcgg cgtacacagc cacgaagatc acatcatggc gatggtagaa ctggcagctg
    21781 aacgcggcgc agaaaaaatc tacctgcacg cattccttga cggtcgcgac accccgccgc
    21841 gcagtgctga atcctcgctg aaaaaatttg aagagaaatt tgctgcgctg ggtaaaggcc
    21901 gcgtagcgtc catcattggt cgttactacg cgatggaccg cgataaccgt tgggatcgcg
    21961 ttgaaaaagc ctatgacctg ctgactctgg cgcagggtga gttccaggcc gatactgccg
    22021 ttgctggttt gcaggcagct tatgctcgcg acgaaaacga cgagttcgtg aaagcgaccg
    22081 ttattcgtgc agaaggtcag ccagatgcgg caatggaaga cggcgatgcg ctgattttca
    22141 tgaacttccg tgctgaccgc gcgcgtgaaa tcactcgtgc attcgtgaac gctgatttcg
    22201 atggcttcgc gcgtaagaaa gtggttaacg tcgatttcgt gatgctgacc gaatacgccg
    22261 ctgacatcaa aactgcggtt gcttacccac ccgcttccct ggttaacacc ttcggcgagt
    22321 ggatggcgaa aaacgacaaa actcagttgc gtatttccga aaccgaaaaa tatgcccacg
    22381 ttactttctt cttcaacggt ggcgtagaag agtcgttcaa aggcgaagat cgcattctga
    22441 tcaactcacc gaaagtggct acctacgatc tgcaaccgga aatgagctcc gcagagctga
    22501 ccgaaaaact ggttgcggcc atcaagagcg gcaaatacga caccattatc tgtaactatc
    22561 cgaacggcga catggtaggt cacaccgggg tgatggaagc ggcggttaaa gcggttgaag
    22621 cactggatca ctgcgtggaa gaagtcgcga aagcggttga gtccgtgggt ggacaactgc
    22681 tgatcaccgc tgaccacggt aacgctgaac agatgcgcga tccggcaacg ggtcaggcac
    22741 acacggcaca caccaacctg ccagttccgc tgatttatgt tggtgataag aacgtgaaag
    22801 cggttgaagg cggcaaactt tctgacatcg cgccgaccat gttgtcgctg atgggtatgg
    22861 aaatcccgca agagatgact ggtaagccgc tgttcatcgt ggaataatcc ctccccatga
    22921 ggggaaaggc gattaatacc atgacacggg ccgtgaaacc gcgcaggttt gcaatcaggc
    22981 ccatcatcta cgccagcgtt cttagcgctg gcgtattgtt gtgcgccttt tccgcccacg
    23041 cggatgagcg tgaccaactc aaatctattc aggccgatat cgccgcaaaa gagcgcgcgg
    23101 tacgccaaaa gcaacaacaa cgcgcaagcc tgctcgcaca attgaaaaag caggaagaag
    23161 cgatctctga agccacccgt aagctgcgcg aaacgcaaaa cacgctcaat caactcaata
    23221 aacagattga tgagatgaac gcgtcgattg ccaaactgga gcagcaaaaa gccgcccagg
    23281 agcgcagcct cgccgcgcaa ctggatgccg cattccgtca gggcgagcat accggtattc
    23341 agctaattct cagcggtgaa gaaagccagc gtggacagcg tttacaggct tatttcggct
    23401 atctcaacca ggcgcgacaa gaaaccattg cccagttgaa gcaaacgcgt gaagaagtcg
    23461 ccatgcagcg tgctgaactg gaagagaaac aaagcgagca acaaacgctg ttatatgaac
    23521 agcgcgccca acaggcgaaa ctgactcagg ctctgaacga gcgtaaaaag acgctggcgg
    23581 ggctggaatc ttccatccag caaggtcagc agcagctaag tgaactgcgt gccaacgaat
    23641 cccgtctgcg taacagcatt gcccgtgcgg aagccgcggc gaaagcgcgt gctgaacgag
    23701 aagcgcgtga ggcccaggcg gttcgcgacc gccagaaaga agcgacgcgc aaaggcacca
    23761 cctataaacc gaccgaaagc gaaaaatcgc tgatgtcccg aactggtggc ctgggtgcgc
    23821 cgcgtggtca ggcattctgg ccggttcgcg ggccgacgct gcatcgctat ggtgaacagc
    23881 tacagggcga actacgctgg aaaggaatgg ttatcggtgc ctctgaaggt actgaagtta
    23941 aagcgattgc cgacggtcgg gtgattctgg ctgactggct gcaaggttac ggtctggtgg
    24001 tggtggttga gcacggtaaa ggcgacatga gtctttacgg ctataatcag agcgcactgg
    24061 tgagcgttgg ttcgcaggtt cgcgcgggcc agccaattgc actggtgggc agcagtggcg
    24121 gtcagggtcg gccttcactc tatttcgaaa ttcgccgcca gggtcaggcg gtcaatccac
    24181 agccgtggtt gggaagataa gttttgtttc catttcgtcg taacgttctt gcatttgccg
    24241 ctctgttggc gctctcctcc cccgtacttg ctggcaaact tgccatcgtc attgatgatt
    24301 ttgggtatcg cccgcacaac gaaaaccagg tgctggcgat gccctccgct atctccgtcg
    24361 ctgtattacc cgattcaccg cacgccagag agatggcgac caaagcgcat aacagcgggc
    24421 acgaagtgtt gattcatctc ccgatggcac cactgagtaa acagccgctg gagaaaaata
    24481 cgctgcgccc ggagatgagc agcgacgaaa ttgagcgcat tattcgtagt gcggtcaata
    24541 acgtgcccta tgccgtgggg atcaacaacc acatgggtag caagatgacc tctaacctgt
    24601 ttggtatgca gaaagtgatg cgggcactgg agcgttacaa tctttacttc ctcgacagcg
    24661 taaccatcgg taatacccag gcgatgcgcg ccgcgcaagg tactggcgtg aaggtgatca
    24721 aacggaaggt gttcctcgac gattcgcaga acgaagcgga catccgtgtg caatttaatc
    24781 gcgcaattga tctggcgcgt cgcaacggtt caaccattgc cattgggcat cctcaccctt
    24841 caacggtacg cgtgctacaa cagatggttt ataacctgcc gccagacatt acgctggtga
    24901 aagccagcag cttgctgaat gaaccgcagg ttgatacttc tacaccgccg aaaaacgctg
    24961 tgcctgatgc accgcgtaat ccgttccgtg gcgtgaagct gtgcaaaccg aagaaaccga
    25021 tagaacctgt ttatgctaat cgcttctttg aagtattaag cgaaagcatc agccagagca
    25081 cgctgatcgt ttacttccag catcagtggc aaggctgggg caaacagccc gaagcggcga
    25141 agtttaacgc tagcgcaaat taaaggcgct acgcgtaatg cgatggtgcg accgcgtttt
    25201 gtcgcgccat tgccataagc ggaaagacca cagtaacgcc tggtaaccga ccttcacgct
    25261 gcgcacattg gtaatcaggc gcttatacat accggaagta aatatctcgg caatcatccg
    25321 ttggcgggta agaatatccg gctctttgcg caccgcatgg caaacacgca atgcttcgta
    25381 agttatttgc tgatgaaatt ccggataaat cgtaattttg tcggcataat ttcgatttaa
    25441 tttctccagc aggcgggtaa tcttaatata gtgacgttga taattaaggt ttttattccc
    25501 ttgtctatgc aaccgactca ctgacgtatt atgcagataa tatttatata atgattgctc
    25561 ggtatatcgc gcacgcagcg cgttaaacat gaattctgtt gtccagacaa tatcctgatg
    25621 atgtaatccg gcaataaatt taatgttatt tttaacaata acatcacggc gataaacccc
    25681 catccagaca acgtgagtcc aacggcgcga agaaagcccc atccgcagcc agtccgggcc
    25741 ggttaatacg ccggttgagc gaaggcgatc gctggggatg gattgccagg tttctcccgt
    25801 ttcacgaaaa caccagtcag cgttgcactg cgccacgtcg aggtcatcct ctaacgccat
    25861 ggtcatcagc gtttcgtaca tggtgggata gacctcatcg tcagcatcga caaaagcgac
    25921 atatttgccc gtcgccacct cgatcccacg attacgcgct accgatgcgc cagcattcgc
    25981 ctgatgcaac aaacgaacgt gcggatagtt ttctgcgtaa tgctttgcta tttcaacaga
    26041 attatccgtt gaaccatcgt taataataat gatttccaga gcagtccagg tttgcgtaat
    26101 taaagattcc atacaagtgc ggaaatcatc gcccgcatta tataacggaa taataacact
    26161 aagtttattg gtgctgttca tcataaatta ccgacgacct ctgaagttat cctgacgttt
    26221 tacaggcttt gtattaagaa actattaagc ctgtgagata ttatttttac aattattttt
    26281 acaggtgaga gagtcacttc agggtagaga gataatgaga gcagctatat aagagatcag
    26341 cgcaattacg atatctttat tacactgaca ggattttaca tatttaacgg attatcctca
    26401 ggcaaaaggg ctggaatacc agcccttgtt cgtgttaatc ccaactcaga attactttcc
    26461 cggactggcc cgaacgcata gcgtcaaagc ccttctggaa atcatcgata gagaaacgat
    26521 gggtaatgat cggcgagaga tccagacctg actgaatcag cgccgccatc ttgtaccagg
    26581 tttcaaacat ctcacgaccg taaatacctt taatgaacaa gcctttaaag attactttgg
    26641 tccagtcgat agacatatcg gacggtggaa tacccagcat cgcaatacga ccgccgtggt
    26701 tcatggtatc gagcatggta cgaaacgctg gcggcgcgcc ggacatttcc agaccgacat
    26761 caaagccttc ggtcatgccc agttcagcca tcacatcatt aaggttttct ttcgcgacgt
    26821 taaccgcacg ggtgataccc attttgcgcg ccagctcaag gcggtattcg ttaacatcgg
    26881 tgatcaccac attgcgtgca ccaacgtgtt tcgccaccgc cgcagccata atacctatcg
    26941 gacctgcacc agaaaccagc acatcttcgc ccaccagatc gaacgacagc gcggtatgca
    27001 cggcgttacc gaagggatca aaaatggaag ccaggtcgtc ggagatattg tctgggattt
    27061 tgaaggcgtt gaacgccggg atcaccagat attcggcgaa gcagcccgga cggttaacgc
    27121 cgacgccgat cgtgttgcgg cacagatggg tacgaccgcc acgacagttg cgacagtgac
    27181 cgcaggtgat atggccttcg ccagaaacgc gatcgccgat tttgaagcct ttcacttcct
    27241 gaccaatacc taccacttca ccgacatatt catggcctac gaccatcggc accgggatgg
    27301 ttttttgcga ccactcatcc cagttataga tgtgaacgtc agtcccgcag atggctgttt
    27361 tacggatttt aatcagcaga tcgttatgcc cgagttccgg tacaggaacg tcggtcatcc
    27421 agatgccctc ttccgctttc agtttggata acgctttcat ctcacatcct caggcgataa
    27481 cgcccagttg tttaccaata cgcgtgaatg cttctactgc acgcgtaatt tgctcagggg
    27541 tatgcgccgc agacatctgg gtacgaatac gcgcctgacc tttcggaacg accggataga
    27601 agaaaccggt gacgtaaatg ccctcttttt gcagctcacg ggcaaatttc tgcgccacta
    27661 ctgcatcgcc aagcatgacc ggaataatgg catgatcggc tcccgccagg gtaaagcccg
    27721 ccgccgacat ttgctcacgg aactggcgcg cgttcgccca cagacggtca cgcagttcgc
    27781 tgcccgcttc gaccatctcc agtactttga tggacgcggc aacaatggcc ggtgccagcg
    27841 agttggagaa caggtacgga cgagaacgct ggcgcagcca ctcaaccacc tctttgcgcg
    27901 ccgcggtata accaccagaa gccccgccca gcgctttacc aagcgtaccg gtgataatat
    27961 cgacccggcc catcacatcg cagtattcat gggaaccacg accattttca ccgacaaaac
    28021 cgaccgcgtg ggagtcgtct accatcacca gggcatcgta tttatctgcc agatcgcaaa
    28081 cgcccttcag attggcaatc acgccgtcca tcgagaatac accatcggtg gcaatcagca
    28141 catgacgcgc accggcttca cgcgcttctt tcagacgagc ttcaagctcc tgcatatcgt
    28201 tgttggcata gcgatagcgt ttagctttac acagacgcac accatcaata atagacgcgt
    28261 ggttcagtgc gtcggagata atggcgtctt ccgcgcccag cagcgtttca aacaggccac
    28321 cgttagcatc aaagcaggaa gagtagagaa tcgcatcttc catccccagg aaggccgcca
    28381 gtttttgttc aagctcttta tggctgtcct gagtgccgca aataaaacgc accgaagcca
    28441 tgccgaaacc gtgagaatcc attcccgcct ttgccgccgc aatcagatca ggatgattcg
    28501 ccagcccgag atagttgttg gcacaaaagt taatgacgtg gcttccatca gccacagtga
    28561 tatctgcttg ctgcgcagac gtaataatgc gctcttcttt aaacaaccct tccgcccgtg
    28621 cggtttccag atcgttggtt aactgctgat aaaattctcc acgcattgcg attctccaga
    28681 cagggcaaat tccagcacat attacccaaa cttataggtc gcgacgagat gacgcgttaa
    28741 cacttctgca aaattcagga taaataacgt gtaacttgat gtctctcaac acctacagat
    28801 agtagaaaaa ctgtacagtt ttggctgggc ataatgatga ttgattttac tgcattgata
    28861 taaaaagaaa aaatgatgcc aaagcatcat ttttcctatc tcagttggta tcaaaacgct
    28921 acccactaat ggagggaaac ctattttctt gaaaggaaaa ttctcagcgc ctgcattttt
    28981 gcccctaaag tgttactcct gaagcagcga aacaaatcga accattttcc tctccccaga
    29041 tagtttaggt tgaagagatc ggggcgtttg taatagcaca tgacaaaaat gccctggtca
    29101 tcatccacaa tgttattatt tagtgttatt ttttgacttt ccagaactaa tttatagaat
    29161 tctttccatt tatgctgtga accaacaatt gcgcccccaa taatatatac atgattgcca
    29221 atcatgaaat caaaaacctg ttgttgagat gtgacagtaa gccctttttt tatagtgaaa
    29281 agatgcatct tactttcatc aaatgggaaa tcccaaattt tcaatcctcg agttacattt
    29341 ggcttacggc aatagccaaa atctatccac gcaacaagag gcgttttcac taagcccatg
    29401 ttgatagctt tatttacaaa ataggcctta aggttacata ccaaaacata ctctggtgac
    29461 cagtactctg ggttttttaa ttgtcgaggc tcaagtctat ttgtaaacga ctcatctttt
    29521 tgaatttttt cgatacgact tctgatatat ctaaatttct tttttatatc gataacgata
    29581 accgttgttg gttttccgtt gcgaatcgct tcaacccttg gtttcaaatc gggagaagtg
    29641 aaaataatca tctcattttc aagtgcggcc agacgctcaa agtaactaaa ataaacatca
    29701 actgaacgag ctagtttttc acggaaccct ttattagcag tccaatcccc tctaccaatg
    29761 tcaaaatatg cagtgataat tgtggtggat gatttcatta ctaataagac cattaatata
    29821 aaaaaagaat tatatcataa atattaacag aatgtatact gtatattatc cattcaaatg
    29881 cttccgttat ttatcataca tcggaatatt gatactaaag cactattcac atgcaaaacc
    29941 aacatccgcc atgaaggact agctaaaacc caaactagtt tgttgcaatt agcatccttg
    30001 cacctctatg taaagggctg aagggattcg gatgtgatgg tatgattaca gacattcgtg
    30061 tctgagattg tctctgactc cataattcga aggttacagt tatgatcatc gttaccggcg
    30121 gcgcgggctt tatcggcagc aacatcgtta aagccctgaa tgataaaggc atcaccgata
    30181 ttctggtggt ggacaacctg aaagacggca ccaagtttgt gaacctggtg gatctgaata
    30241 tcgcagacta tatggataag gaagacttcc tgatccagat tatggctggc gaagagttcg
    30301 gcgatgtcga agcgattttc cacgaaggcg cgtgctcttc caccaccgag tgggacggca
    30361 agtatatgat ggataacaac tatcaatact ccaaggagct gctgcactac tgcctggagc
    30421 gtgaaatccc gttcctgtac gcttcttccg cagccaccta cggcggacgc acctccgact
    30481 ttattgaatc ccgcgagtac gaaaaaccgt tgaacgtcta cggttactca aaattcctgt
    30541 ttgatgaata tgttcgccaa atcctgccgg aagcgaactc gcagattgtt ggcttccgct
    30601 atttcaacgt ttatggaccg cgtgaaggcc ataaaggcag catggcgagc gtcgctttcc
    30661 atctcaacac ccagcttaat aacggtgaat caccgaagct gttcgaaggt agcgagaact
    30721 ttaaacgcga cttcgtctat gttggcgatg tggcagatgt aaacctgtgg ttcctggaaa
    30781 atggcgtttc cggcatcttc aacctcggca ccggtcgtgc ggaatccttc caggctgtag
    30841 ctgatgctac gctggcttat cacaagaaag gccagatcga atacattccg ttcccggata
    30901 agctgaaagg ccgctaccag gcgttcactc aggcagatct gacaaatctg cgcgcggcgg
    30961 gttacgacaa accgttcaaa accgttgctg aaggtgtaac ggaatacatg gcctggctga
    31021 atcgcgacgc ataagagctc tgcatgaaaa tactggtgat cggcccgtct tgggttggcg
    31081 acatgatgat gtcgcaaagt ctctatcgca cgctccaggc gcgctatccc caggcgataa
    31141 tcgatgtgat ggcaccggca tggtgccgtc cattattatc gcggatgccg gaagttaacg
    31201 aagctatccc tatgcctctc ggtcacggag cgctggaaat cggcgaacgc cgcaaactgg
    31261 gtcatagcct gcgtgaaaag cgctacgacc gcgcctacgt cttacctaac tccttcaaat
    31321 ccgcattagt gcctttcttc gcgggtattc ctcatcgcac cggctggcgc ggcgagatgc
    31381 gctacggttt actcaacgat gtacgcgtgc tcgataaaga agcctggccg ctaatggtgg
    31441 aacgctatat agcgctggcc tatgacaaag gcattatgcg cacagcacaa gatctgccgc
    31501 agccattgtt atggccgcag ttgcaggtga gcgaaggtga aaaatcatat acctgtaatc
    31561 aattttcgct ttcatcagaa cgtccgatga ttggtttttg cccgggtgcg gagtttggtc
    31621 cggcaaaacg ctggccacac taccactatg cggagctggc aaagcagctg attgatgaag
    31681 gttatcaggt ggttctgttt ggctcggcga aagatcatga agcaggcaat gagattcttg
    31741 ccgctttaaa taccgagcag caggcatggt gtcggaacct ggcgggggaa acacagcttg
    31801 atcaagcggt tatcctgatt gcagcctgta aagccattgt cactaacgat tctggcctga
    31861 tgcacgttgc ggcggcgctc aatcgtccgc tggttgccct gtatggtcca agtagcccgg
    31921 acttcacacc gccgctatcc cataaagcgc gcgtgatccg tctgattacc ggctatcaca
    31981 aagtgcgtaa aggtgacgct gcggagggtt atcaccagag cttgatcgac attactcccc
    32041 agcgcgtact ggaagaactc aacgcgctat tgttacaaga ggaagcctga cggatgcggg
    32101 ttttgatcgt taaaacatcg tcgatgggcg atgttctcca tacgttgccc gcactcactg
    32161 atgcccagca ggcaatccca gggattaagt ttgactgggt ggtggaagaa gggttcgcac
    32221 agattccttc ctggcacgct gccgttgagc gagttattcc tgtggcaata cgtcgctggc
    32281 gtaaagcctg gttctcggcc cccataaaag ctgaacgcaa agcgtttcgt gaagcgctac
    32341 aagcagagaa ctatgacgca gttatcgacg ctcaggggct ggtaaaaagc gcggcactgg
    32401 tgacacgtct ggcgcatggc gtaaagcatg gattggactg gcaaaccgct cgcgaacctt
    32461 tagccagcct gttttacaat tgtaagcatc atattgcaaa acagcagcac gccgtagaac
    32521 gcacccgcga actgtttgcc aaaagtttgg gctatagcaa accgcaaacc cagggcgatt
    32581 atgctatcgc acagcatttt ctgacgaacc tgcctacaga tgctggcgaa tatgccgtat
    32641 ttcttcatgc gacgacccgt gatgataaac actggccgga agaacactgg cgagaattga
    32701 ttggtttact ggctgattca ggaatacgga ttaaacttcc gtggggcgcg ccgcatgagg
    32761 aagaacgggc gaaacgactg gcggaaggat ttgcttatgt tgaagtattg ccgaagatga
    32821 gtctggaagg cgttgcccgc gtgctggccg gggctaaatt tgtagtgtcg gtggatacgg
    32881 ggttaagcca tttaacggcg gcactggata gacccaatat cacggtttat ggaccaaccg
    32941 atccgggatt aattggtggg tatgggaaga atcaagtaga gtgtcgttct acaagtatgt
    33001 ctcttgcaga tttgccagct caaaccgttt ttcaaaattt aaatcttgaa ataataacca
    33061 ataagttgac atcggagata agatgacctc aacattattt ttctctctcg agaaaaaaaa
    33121 ctggatagcg tactggaaca gagctctcgt attcttattc attaccacct attttttggg
    33181 tgggattaca aggtataaac atcttattgt tattcttatg acaataacga caatcgtcta
    33241 tctctgcaaa cggccaaaac actatctctc actatttaaa acatttcttt ttggtagtgt
    33301 tgccatatta actattgctg cattgctgtc acttcttcaa tcccctgatg caggtgctag
    33361 catgaaggaa attttcaaag ctattattga gaatacttta ctatgcacaa tagcaatacc
    33421 gatcatattg agagacgaga aaagagaaga tgtcgaaaaa atcgttttct tctcatttat
    33481 tagtgcgttg ggcttacgct gtttttctga attgattacc tattataagg actatcaaca
    33541 agggataatg ccattcgcag attatagaca ccgtagcatt tctgactcga tggtcttttt
    33601 attccctgca ttgttaaatc tctggcttat caaatcagca aaataccgca tttcttttgt
    33661 ggttctaagc gttattttta tttttctgat attaggaact ttatccagag gggcctggct
    33721 ttccgtgtta gtcattggat taatatggat tctgatgttt aaacaatgga agttactatt
    33781 agtaggagta atggttgcca tcattgcatt gtcggttatt ttcacacata aggagatgac
    33841 cgcaaagcta acgtataaac ttcaacaaac taatagttct tatcgctatg caaatggtac
    33901 tcaaggcagc gcactcgatc taatattaga aaatcctgtt attggttatg gttacggtaa
    33961 cgttgcatat aaagatgtct ataataaacg tgtcattgat tatccagaat ggacctttag
    34021 acaatcaata gggccacata attttgcgct attcatctgg tttggcactg gtttattagg
    34081 gctggtaagt cttatgatgc tatactgtgc aatattgaaa gagtgtataa aaaat
//
LOCUS       DAZFWL020000040        33727 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFWL020000040 DAZFWL020000000
VERSION     DAZFWL020000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 33727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.040"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(37..273)
                     /gene="yjjZ"
                     /locus_tag="ACHKNP_RS10205"
                     /old_locus_tag="pgaptmp_002041"
     CDS             complement(37..273)
                     /gene="yjjZ"
                     /locus_tag="ACHKNP_RS10205"
                     /old_locus_tag="pgaptmp_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HGU4704210.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMLMNLNQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="ACHKNP_RS10210"
                     /old_locus_tag="pgaptmp_002042"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="ACHKNP_RS10210"
                     /old_locus_tag="pgaptmp_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HGU4704211.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="ACHKNP_RS10215"
                     /old_locus_tag="pgaptmp_002043"
     CDS             complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="ACHKNP_RS10215"
                     /old_locus_tag="pgaptmp_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HGU4704212.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMTRISFAAVIFSFSAMRSERREGLSCLTELAIKLPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1875..2600)
                     /gene="yjjQ"
                     /locus_tag="ACHKNP_RS10220"
                     /old_locus_tag="pgaptmp_002044"
     CDS             complement(1875..2600)
                     /gene="yjjQ"
                     /locus_tag="ACHKNP_RS10220"
                     /old_locus_tag="pgaptmp_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4704213.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3219..3989
                     /gene="yjjP"
                     /locus_tag="ACHKNP_RS10225"
                     /old_locus_tag="pgaptmp_002045"
     CDS             3219..3989
                     /gene="yjjP"
                     /locus_tag="ACHKNP_RS10225"
                     /old_locus_tag="pgaptmp_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HGU4704214.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            3980..4453
                     /gene="yjjB"
                     /locus_tag="ACHKNP_RS10230"
                     /old_locus_tag="pgaptmp_002046"
     CDS             3980..4453
                     /gene="yjjB"
                     /locus_tag="ACHKNP_RS10230"
                     /old_locus_tag="pgaptmp_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HGU4704215.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4560..5099
                     /gene="dnaT"
                     /locus_tag="ACHKNP_RS10235"
                     /old_locus_tag="pgaptmp_002047"
     CDS             4560..5099
                     /gene="dnaT"
                     /locus_tag="ACHKNP_RS10235"
                     /old_locus_tag="pgaptmp_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HGU4704216.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5102..5839
                     /gene="dnaC"
                     /locus_tag="ACHKNP_RS10240"
                     /old_locus_tag="pgaptmp_002048"
     CDS             5102..5839
                     /gene="dnaC"
                     /locus_tag="ACHKNP_RS10240"
                     /old_locus_tag="pgaptmp_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HGU4704217.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            5888..6382
                     /gene="yjjA"
                     /locus_tag="ACHKNP_RS10245"
                     /old_locus_tag="pgaptmp_002049"
     CDS             5888..6382
                     /gene="yjjA"
                     /locus_tag="ACHKNP_RS10245"
                     /old_locus_tag="pgaptmp_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HGU4704218.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6636..8927
                     /gene="opgB"
                     /locus_tag="ACHKNP_RS10250"
                     /old_locus_tag="pgaptmp_002050"
     CDS             6636..8927
                     /gene="opgB"
                     /locus_tag="ACHKNP_RS10250"
                     /old_locus_tag="pgaptmp_002050"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HGU4704219.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGAGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9066..10088)
                     /gene="lgoD"
                     /locus_tag="ACHKNP_RS10255"
                     /old_locus_tag="pgaptmp_002051"
     CDS             complement(9066..10088)
                     /gene="lgoD"
                     /locus_tag="ACHKNP_RS10255"
                     /old_locus_tag="pgaptmp_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HGU4704220.1"
                     /translation="MSTMKVLICQEIKNLVWTVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNFHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            10227..11132
                     /locus_tag="ACHKNP_RS10260"
                     /old_locus_tag="pgaptmp_002052"
     CDS             10227..11132
                     /locus_tag="ACHKNP_RS10260"
                     /old_locus_tag="pgaptmp_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HGU4704221.1"
                     /translation="MSRSQNLRHNVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(11303..12955)
                     /locus_tag="ACHKNP_RS10265"
                     /old_locus_tag="pgaptmp_002053"
     CDS             complement(11303..12955)
                     /locus_tag="ACHKNP_RS10265"
                     /old_locus_tag="pgaptmp_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HGU4704222.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRTSTENLDNELIMETGARVIGYMRRSEDDMSENDNSLHMQRAKLERTFNL
                     AALNARRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
     gene            13635..14606
                     /locus_tag="ACHKNP_RS10270"
                     /old_locus_tag="pgaptmp_002054"
     CDS             13635..14606
                     /locus_tag="ACHKNP_RS10270"
                     /old_locus_tag="pgaptmp_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HGU4704223.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
     gene            14670..15143
                     /locus_tag="ACHKNP_RS10275"
                     /old_locus_tag="pgaptmp_002055"
     CDS             14670..15143
                     /locus_tag="ACHKNP_RS10275"
                     /old_locus_tag="pgaptmp_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HGU4704224.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVMTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVAVLSIALLLYMAWLG
                     YRLAEMAQFKVTQLLKVSWFWIDLAVPVGALATAIYTLYWLIHDIKQSDSGDKMQ"
     gene            15140..16411
                     /locus_tag="ACHKNP_RS10280"
                     /old_locus_tag="pgaptmp_002056"
     CDS             15140..16411
                     /locus_tag="ACHKNP_RS10280"
                     /old_locus_tag="pgaptmp_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HGU4704225.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAAAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLTLVTYIPAISLFLV"
     gene            complement(16454..18118)
                     /gene="tsr"
                     /locus_tag="ACHKNP_RS10285"
                     /old_locus_tag="pgaptmp_002057"
     CDS             complement(16454..18118)
                     /gene="tsr"
                     /locus_tag="ACHKNP_RS10285"
                     /old_locus_tag="pgaptmp_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HGU4704226.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSGENWETF"
     gene            complement(18237..18683)
                     /gene="hpaR"
                     /locus_tag="ACHKNP_RS10290"
                     /old_locus_tag="pgaptmp_002058"
     CDS             complement(18237..18683)
                     /gene="hpaR"
                     /locus_tag="ACHKNP_RS10290"
                     /old_locus_tag="pgaptmp_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HGU4704227.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            18955..20244
                     /gene="hpaG"
                     /locus_tag="ACHKNP_RS10295"
                     /old_locus_tag="pgaptmp_002059"
     CDS             18955..20244
                     /gene="hpaG"
                     /locus_tag="ACHKNP_RS10295"
                     /old_locus_tag="pgaptmp_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HGU4704228.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGCLVNRIVSEETAK"
     gene            20241..21707
                     /gene="hpaE"
                     /locus_tag="ACHKNP_RS10300"
                     /old_locus_tag="pgaptmp_002060"
     CDS             20241..21707
                     /gene="hpaE"
                     /locus_tag="ACHKNP_RS10300"
                     /old_locus_tag="pgaptmp_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4704229.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            21709..22560
                     /gene="hpaD"
                     /locus_tag="ACHKNP_RS10305"
                     /old_locus_tag="pgaptmp_002061"
     CDS             21709..22560
                     /gene="hpaD"
                     /locus_tag="ACHKNP_RS10305"
                     /old_locus_tag="pgaptmp_002061"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HGU4704230.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            22570..22950
                     /locus_tag="ACHKNP_RS10310"
                     /old_locus_tag="pgaptmp_002062"
     CDS             22570..22950
                     /locus_tag="ACHKNP_RS10310"
                     /old_locus_tag="pgaptmp_002062"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HGU4704231.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQHDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            23061..23864
                     /gene="hpaH"
                     /locus_tag="ACHKNP_RS10315"
                     /old_locus_tag="pgaptmp_002063"
     CDS             23061..23864
                     /gene="hpaH"
                     /locus_tag="ACHKNP_RS10315"
                     /old_locus_tag="pgaptmp_002063"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HGU4704232.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            23875..24663
                     /gene="hpaI"
                     /locus_tag="ACHKNP_RS10320"
                     /old_locus_tag="pgaptmp_002064"
     CDS             23875..24663
                     /gene="hpaI"
                     /locus_tag="ACHKNP_RS10320"
                     /old_locus_tag="pgaptmp_002064"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HGU4704233.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAIKPDVY"
     gene            24838..26214
                     /gene="hpaX"
                     /locus_tag="ACHKNP_RS10325"
                     /old_locus_tag="pgaptmp_002065"
     CDS             24838..26214
                     /gene="hpaX"
                     /locus_tag="ACHKNP_RS10325"
                     /old_locus_tag="pgaptmp_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HGU4704234.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVATLLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            26224..27114
                     /gene="hpaA"
                     /locus_tag="ACHKNP_RS10330"
                     /old_locus_tag="pgaptmp_002066"
     CDS             26224..27114
                     /gene="hpaA"
                     /locus_tag="ACHKNP_RS10330"
                     /old_locus_tag="pgaptmp_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HGU4704235.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            27365..28927
                     /gene="hpaB"
                     /locus_tag="ACHKNP_RS10335"
                     /old_locus_tag="pgaptmp_002067"
     CDS             27365..28927
                     /gene="hpaB"
                     /locus_tag="ACHKNP_RS10335"
                     /old_locus_tag="pgaptmp_002067"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HGU4704236.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            28945..29457
                     /locus_tag="ACHKNP_RS10340"
                     /old_locus_tag="pgaptmp_002068"
     CDS             28945..29457
                     /locus_tag="ACHKNP_RS10340"
                     /old_locus_tag="pgaptmp_002068"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HGU4704237.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSIT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            29850..32000
                     /gene="btsT"
                     /locus_tag="ACHKNP_RS10345"
                     /old_locus_tag="pgaptmp_002069"
     CDS             29850..32000
                     /gene="btsT"
                     /locus_tag="ACHKNP_RS10345"
                     /old_locus_tag="pgaptmp_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HGU4704238.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            32131..32334
                     /gene="yjiX"
                     /locus_tag="ACHKNP_RS10350"
                     /old_locus_tag="pgaptmp_002070"
     CDS             32131..32334
                     /gene="yjiX"
                     /locus_tag="ACHKNP_RS10350"
                     /old_locus_tag="pgaptmp_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU4704239.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            32345..33301
                     /gene="yjiA"
                     /locus_tag="ACHKNP_RS10355"
                     /old_locus_tag="pgaptmp_002071"
     CDS             32345..33301
                     /gene="yjiA"
                     /locus_tag="ACHKNP_RS10355"
                     /old_locus_tag="pgaptmp_002071"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGU4704240.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(33388..33579)
                     /locus_tag="ACHKNP_RS10360"
                     /old_locus_tag="pgaptmp_002072"
     CDS             complement(33388..33579)
                     /locus_tag="ACHKNP_RS10360"
                     /old_locus_tag="pgaptmp_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HGU4704241.1"
                     /translation="MSMVKHKRGTLYTLDVQQEAELMALVNKSDEDIDYSDIPASGSE
                     AWSDAKRGNFLPDPGIHAQ"
     gene            complement(33619..>33727)
                     /locus_tag="ACHKNP_RS10365"
                     /old_locus_tag="pgaptmp_002073"
     CDS             complement(33619..>33727)
                     /locus_tag="ACHKNP_RS10365"
                     /old_locus_tag="pgaptmp_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="membrane domain protein"
                     /protein_id="HGU4704242.1"
                     /translation="GKWGVNELKCKKGSDRRDAYSIENVSEYNVPGWLK"
BASE COUNT         8320 a         8866 c         8446 g         8095 t
ORIGIN      
        1 tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattag
       61 catcagcatg atgccagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
      121 ctgtttgtga tacagcatca ccggcgtcaa tatcaatccc atcagaatga caatccgcca
      181 ttgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aaccaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      421 atcgttccgc accgctctat gaagatgtca tctggcgaac gcatctccag ccgcaggatg
      481 ctgggcttgc acaagccgta cgggcgacga tcgccgaaca tcgtgaacat ttgctggagt
      541 ttatccgtct ggatgaaccc gccccgcttc gcgccatgac gctggcgcaa tggtcatcac
      601 cgaatgcgct aagttctcta ctggcggtct attccgatca tatctatcgc aaccaaccaa
      661 cgatgatccg cgagaacaag ccgctgatct cactatgggc gcaatggtat atcggcctga
      721 tggtgccacc attaatgctg gcgctgctga cgcaggaaaa agcgttagat gtgtcgccgg
      781 aacatttcca cgttgagttt cacgaaaccg gccgtgccgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg ttaatcagcc
      901 aggcgctggt tccggttgtg caggcactag aagcgacggg agagatcaac ggcaaactta
      961 tctggagtaa taccggttat ttgatcaact ggtatctgac tgagatgaaa cagctgctcg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg cttactaccg
     1081 gtgaagataa tccactctgg cgtaccgtgg tactgcgcga cggcctgctg gtgcgccgca
     1141 cttgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1201 gaaagcactg ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt
     1261 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca accctgcatc
     1321 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1381 acgtttaagc tgctcggcaa tttgtgtcat tgagtaacca cgcgacataa agcgcaatat
     1441 ttctctctcc gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg
     1501 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1561 cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg
     1621 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaagct taatcgccag
     1681 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaaat
     1741 gaccgccgca aacgagatcc gcgtcatggc atcctgaaat gactcctgac tggtataggt
     1801 atggagctta taagggttac ccgcaaactt tctgaataaa ctctcaatac cgatactact
     1861 catgacgcat ttttctatga gtgcgacatt tctcttctta attcggctgt gttccattct
     1921 gcggcatctc cataaacaga aggcaagtta agttctcttg caccctgcgc gctatttatc
     1981 catgcataca tttcagcgtt gctgtgaatt gccagtcgac gcatcgcact gtttttttgg
     2041 gcgctgatag ttttattact tttcttaagc aatgaagcta tctggttgat gccccatcct
     2101 ttacctaata gccgcagtac cttgcgctct gaaagagtga gaatgacgga gcgatagcta
     2161 aaatcatgag tttcagtcat tgccggagag gtcagcatgg cgctgatacg ctctgcgtgc
     2221 gtattgccgg aacgtacggt cttgaccaga ttctcaatgg gttcaacatc agacaataac
     2281 gtcgccgtag ggcacatgag cagttctact gcctgggaat accaggagcg tgacaccatg
     2341 aaaacccagt gaatttcccg atattgtgag attaaagaat aataatgttc acaaacagaa
     2401 cgtggatctt cactttcacc ggctaaatca gcaatgacta atccggaacg gcgtaattgt
     2461 aataaggtaa ggtcctccgc agaggcgctg gatattattt catattcagg gaagtgagtc
     2521 ctcatgacct cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt
     2581 ttgcagcatc ctggcaacat attccctcca catccactgt ctaaaaatta tgatggttta
     2641 ttcaggatat tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat
     2701 ccttagcatt cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt
     2761 cagaaggtta gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta
     2821 cattaagttg ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt
     2881 aaatgatatc ccttcctgca gaatgcaaaa tcacacaacc aatatttatt caatgaaatc
     2941 tgatggtttt aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt
     3001 aaaggccaaa caataaattc attatgatga taatattcat gtgatagttt gtttgcggcg
     3061 agagataatt cgctttttat caccgagctt ctcccgataa ccaggattaa tcagcgcaat
     3121 tttgatctta acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc
     3181 tgaacaatac gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag
     3241 ccgtaacacg gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg
     3301 cgttggttga tgagctttcc tcacgactgg gtcgggcgct gggaatggac agcgtcgaaa
     3361 gttctatctc ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat
     3421 cgacacgtaa aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca
     3481 ttgtgattct tgcggagcat catctgctgg attacaaagg cgtagagaaa cgatttagcc
     3541 aaattcagcc attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg
     3601 cctgtttctg taaactcaat aacggtggct gggatggtgc cgtcatcacc ttctttgcca
     3661 gtacgaccgc gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccacagatca
     3721 acttttgcct taccgctttc gccgccacca ccatttccgg attgcttttg caactcccca
     3781 ctttcagcga tacacccacc attgcgatgg ccgccagcgt tctgctgctg gtgccgggct
     3841 ttccgttgat taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac
     3901 gctgggcgat cgccagtctg ctgacgctgg ctacctgcgt cggcgtggtg atggcactga
     3961 cgatttgggg gctacgcgga tgggtgtgat cgaatttctg ttagcgttgg cgcaggatat
     4021 gatcctcgcc gccattcctg cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc
     4081 gttacgctgg tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac
     4141 cagcgggctg aatattgagt ggtcaacttt tatggcttct atgctggtcg gcaccattgg
     4201 tattcaatgg tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat
     4261 ccctatgttc ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca
     4321 gttaggttac agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc
     4381 gattgttggt gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg
     4441 ccctcgcgta taaaattgcc tcgcttaagc acacggatga gagacagcct cctctcctcc
     4501 gtgtgttact ataaaagtta tctcccttct cgttcatcgt tccatatttg agaaacagta
     4561 tgtcttccag agttttgacc ccggacgtcg ttggtattga cgccctggta cacgatcacc
     4621 aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt atttgctaac aatgccccgg
     4681 cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa gaaaagctgg
     4741 cgcgtccggg aagcgatgtc gctctggacg atcaactcta tcaggaaccg caagccgctc
     4801 ccgttgctgt ccccatgggg aaattcgcca tgtatccgga ctggcaaccc gatgccgact
     4861 ttatccgcct ggcggcgcta tggggcgtgg cgctaagaga gccggtgacc accgaagaac
     4921 tggcctcatt cattgcctac tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc
     4981 aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac
     5041 gagatgtgaa tacggtcagc gaacctgaca gccaaattcc accaggattc agagggtaac
     5101 gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa
     5161 acctgcattc aaaacgggtg aagagctact ggcctggcag aaagaacaag gggcgatccg
     5221 ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc
     5281 cggtattcgt ccactgcatc agaactgctc ctttgagaac tatcgcgttg agtgtgaagg
     5341 gcagatgaat gcgttaagca aagcgcgcca gtatgtcgaa gagtttgacg gcaacatcgc
     5401 cagcttcatc ttttctggta agccaggaac cggcaaaaac catctggcgg cggcaatctg
     5461 caacgagctg ctgctacgcg gtaaatccgt attgatcatc accgtggccg atattatgtc
     5521 ggcgatgaaa gataccttca ggaatagcgg taccagcgaa gaacaactgc ttaacgatct
     5581 gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg cagaccgaat cgaaatacga
     5641 aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat
     5701 gctgaccaac agtaatatgg aagagatgac caagttgctg ggcgaacgcg tgatggaccg
     5761 tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg gatagctacc gtagccgggt
     5821 aacaggtaaa gagtattaat ccccgtttct ggcggtatac tgcgacacaa ttccaccttg
     5881 agtaatgatg atgaaaactg ttaaacatct tctgtgctgt gccattgccg ccagcgcatt
     5941 aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga
     6001 acttggcaac caaaacagca cgacacagga aggcggttgg tcgctcgcgt cattaactaa
     6061 cttgcttagc agcgggaacc aggccttaag cgcagataac atgaacaacg ccgcaggcat
     6121 tctgcaatac tgcgcgaagc aaaagctggc gtcggtaacc gatgccgaaa acatcaagaa
     6181 ccaggtgctg gaaaagctgg gcctgaacag tgaagaacaa aaagaagaca ccaactatct
     6241 ggatggcatt cagggtttgc tgaaaaccaa agatggtcag caactcaatc tggataacat
     6301 cggaacgact ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca
     6361 ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta cccggctgac
     6421 gcggctctct cctgcccttt tccctgctcc tggacggttt tccccctgtt acacgggaag
     6481 cgaccgcgat caaaaaagtc tgtttataaa ctaattccta acaacatcgc atttttttga
     6541 cactagaatt gcagcaatat ggcgacgcca ttacattgtg cttctcaaaa agcttaaacg
     6601 ttggctaaac ggcccggttt agtgaggatc gcccgttgtc agaactactc tctttcgccc
     6661 tttttctcgc ctctgtgctg atttacgcat ggaaagcggg acgtaacacc tggtggtttg
     6721 cagccacatt aacggtgctg gggctatttg tcgttttaaa tatcaccctg tttgccagcg
     6781 actattttac tggcgctggt attaacgacg cggttctcta taccttaacc aacagcctga
     6841 ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg cattgtgctg gggctgacag
     6901 cggtgttcgg tgcgctgggc tggatcctgc gccgtcgtcg ccatcatccg caccattttg
     6961 gttacagcct gctggcgctc ttactggcgc tgggttcggt ggacgccagc ccggcatttc
     7021 gtcagataac ggaactggtg aaatcccagt cacgcgatgg cgacccggac tttgcggctt
     7081 attataaaga gccgtcgaaa actatccctg acccgaaact caacctggtt tatatctacg
     7141 gcgaaagtct cgagcggacc tattttgata acgaagcttt cccggatctc acgcctgaac
     7201 tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac gcagcagctg ccgggaacag
     7261 attacacgat tgcgggcatg gtggcttctc agtgcggcat tccgcttttt gccccatttg
     7321 aaggtaacgc ctccgcttct gtttccagct tcttcccgca gaacatctgt ctgggcgata
     7381 tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg cgcgaatctg cgttttgccg
     7441 gtaaagatgt gttcctgaag tcgcatggct tcgaccactt atacggctca gaagagctga
     7501 aaagcgtggt agccgacccg cactatcgca acgactgggg attctacgac gataccgttc
     7561 tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc aggtcagcga ttctcactgt
     7621 ttaccctgac agtcgatacc catcacccgg atggttttat ctcgcgtacc tgtaaccgca
     7681 aaaaatatga ttttgacggt aagccgaacc agtcattcag cgcggtaagt tgcagccagg
     7741 agaatatcgc ggcgtttatc aacaaaatca aagcgtcacc gtggtttaaa gatactgtca
     7801 tcgtcgtctc ttctgaccat ttagcgatga acaacacggc gtggaaatac ctcaataagc
     7861 aggatcgcaa taacctgttt tttgtcattc gtggcgacaa gccgcagcaa gagacgctgg
     7921 cagtgaagcg taacacgatg gataacggcg cgacggtgct ggacattctc ggtggcgata
     7981 actatctcgg acttggtcgt agcagtttat ccgggcagtc aatgtcggaa atcttcctca
     8041 atatcaaaga gaaaacattg gcgtggaagc cggatatcat ccgcctgtgg aaattcccta
     8101 aagagatgaa agagttcacc atcgaccagc agaaaaacat gattgccttc tcgggtagcc
     8161 atttccgttt gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg ctgccggaaa
     8221 gcgaatactc agcaccgctg cgtttccagc tggccgattt cgctccacgc gacaatttcg
     8281 tctgggttga ccgttgctac aagatggcac aactctgggc tccggaactg gcactttcca
     8341 ccgactggtg tgtctcgcag gggcagcttg gcggtcagca aattgttcag catgttgaca
     8401 aaacaacatg gcagggcaaa acggcattta aagatacggt catcgacatg gcgcgttaca
     8461 aaggcaatgt cgatacgctg aagattgttg ataacgatat tcgctacaaa gccgacagtt
     8521 tcatcttcaa cgtcgccggt gcgccagaag aggtgaaaca gtttagtggg atttcccgtc
     8581 cagagtcgtg gggccgctgg tccaacgcgc agctgggcga tgaagtaaaa atcgagtaca
     8641 agcatccgct gccgaagaaa tttgacctgg tgattaccgc caaagcatac ggcaataacg
     8701 ccagccgtcc tattccggta cgcgtaggca atgaagaaca aacccttgtg ctgggcaatg
     8761 aagtgaccac caccacactg catttcgata acccaaccga tgccgacaca ctggtaattg
     8821 tgccgccgga acctgtctca accaacgaag ggaatatcct cggacactcg ccgcgtaagc
     8881 tcgggatcgg catggtggaa attaaagttg tggaacgtga agggtaattc ctgactgatg
     8941 tgtaggtcgc ataaggtgcg acctacacac cttctccggc ctacaaaaca tcattgagaa
     9001 acagaaaaaa acagcgccca acacttcccc ggacgctgca tggcaacttt aataatctcg
     9061 ccttatcaga aagtaattac acctttaatt aactcacgat tgttaatcac atcgcgctcg
     9121 taggtttctg ccagcgtggc gaacggatag cgatgggtta acatcatgtc agcagtgatt
     9181 tttccttccg ccatcagtcg accgacttta gcaaaatctt ccggcgtggc gttgcggctg
     9241 cccatcatcg tcgtttcttt tttatggaat tccggatcgg agaactgcaa ctcaccttta
     9301 aacaggccga caaataccac cgtgccgccg tgacgaatca gattcacggt gttattcatc
     9361 gcatgttgat tacctgtcgc gtcgatcact ttctgcgcca gcgaaccacc aaactgcgcc
     9421 cgcaactgcg cgtcaaaatc ttcggctgac gggtccagca cgggcaactc cagacgcgtt
     9481 gccacatgtt cacggcgcgc cggactggta tccgccacca ccacctgtgc gccatcggct
     9541 ttagcgattg ccgccgcgcc cagaccgatt ggccccgccc cgaccaccag cacctgctcg
     9601 ccgggagcaa tggctgcgcg acgcaccgca tgagcgctaa tagcgaaagg ttcaatcaat
     9661 gcggccgcct gcgggtcaat accgtctgcg ggcaaaatgt tcgccaccgg cacgctcaaa
     9721 tactcactaa aaccgccatc ctgatgcacg ccaatgactg aaattttttc acagcaattg
     9781 gtacgcccgc ttttacacgc cgggcattgc tgacaagaaa tatacggaat taccgcaact
     9841 tgctgtccga tattaaaatt gtgaaagttt tttcctgatt caataatctc cccacatatt
     9901 tcatggccta aaacgcgtgg atagctaaaa aatggttgat tgcccgccca ggcatgaata
     9961 tctgtaccgc aaatcccgac ggttttaatt ttaattaatg cttcattatc gcctggcacc
    10021 ggtctttccc ggactgtcca tactaaattc tttatttcct gacaaattaa tactttcatt
    10081 gtggacatga ttcaccgctc cttttatttt atgactttca gatatcgtcg aatttataag
    10141 agcacctgaa tttacccata aaatatgcgg atgatcacgc ataaaaatgt tttaaatcgg
    10201 tttttaatgc cggactggag ggaagaatga gtcgttcgca gaatttacgt cataacgtca
    10261 taaatcaggt gattgaagat atggcgaggg gcaatattcc ttctccgctg ccttcacaaa
    10321 gtgggctggc agaaatgtat aacatcagtc gcactacggt acgccacata ctccagcatt
    10381 taagcgcatg tggcgtgctc accctggtgg ggaaaaatta tgttatcgcc cgcaaacccg
    10441 aacagaacga tggatttgat tgcatcacgg cctcgctgaa agagcaaaac cgtcttttcg
    10501 agcaggcatt tttcaccatg attaacctgc gccagttacg cgcgggagaa agcttttccg
    10561 aacttcagct ggcgcgagcg gcaggcgtca gtccggtagt agtacgagaa tatcttttga
    10621 aattcggtcg ttataatctt attcaaagcg aaaagcgcgg ccagtggagc atgaagcagt
    10681 tcgatcaatc ctacgccgag cagctgtttg agctacgcga aatgctcgaa acacattccc
    10741 tgcaacactt tctcaacctt cccgatgacg atccccgctg gttgcaggcg aaaaccatgc
    10801 tcgaacgtca caggacattg cgcgacagca ttggcagcaa ctttcggatg ttttctcaat
    10861 tagaccgtga ttttcatgcc atgctgttat ccgctgctga caacatcttt tttaatcaat
    10921 cacttgaaat tatctcggtg atctttcatt ttcactatca atgggatgaa agcgatctca
    10981 agcagcgcaa tatcattgct gttgatgagc atatgaccat tctcagcgcc ctgatttgcc
    11041 gtagcgattt agatgcaata acggcattgc gaaaccatct ggataccgcc aaacaatcaa
    11101 tgattcgttc catcaggcaa catcaccgat aaattaagta cagattaaaa acattgattc
    11161 ttggtttata tcactatgtt gtattaatgc agtattaatc caggcgattg ttctgcccat
    11221 aaggaaatac atagggaata aacgatgctg gaagtattac tctcagccat catttaactc
    11281 cctattactg gtagcgataa aattatcggc tatccatgat taacgtttcc agcacatcta
    11341 attcactgac tatttttgcc agtgtttcat cactgacttc ttgccgtgca cgcatctggt
    11401 aaatagccgc acgcctggca tttaacgctg ccagattaaa tgttctttcc agcttcgcgc
    11461 gctgcatatg taaagaatta tcattttccg acatatcatc ttcgctacgc cgcatataac
    11521 cgataactct ggcccctgtt tccataatca actcgttgtc gaggttttct gtactggtgc
    11581 gtagcaaacg ttgctcagtc ttttgcacac tgagtatggc cattttggcc ataaacagat
    11641 cggcagcttc ctcttctttt tgccggttat cgctgaaaat gcccacattt ttccccagta
    11701 gtaacggcag aataataatg ccgcaaacca gcgaaaagag gatcacacct gtggcgagaa
    11761 ataccagtaa gtaacgcccg ggaaaaggtg tgccatccgg cagcaataac gggatagaaa
    11821 gcacccccgc aagcgtgatg gctccgcgca cccccgcaaa ggtcgccagc agcatatgac
    11881 gcgttttata tccagcaaac agcatcggat gtttgccggg tcgtttacgg ctatacagac
    11941 gcatcagcca aagccagccc aggcgcacaa ccattaacgc caggtagatc caaatgacat
    12001 caaggagcag cgatccggtg tctaccagtg gatcgttttc tgcacggatc agtgattccg
    12061 aaataatgcc cggcagttgt aagcctaaaa tcagaaacac catgccgtta aagacatatt
    12121 caagcatcga ccagctactt ttagctttaa ttctcaggcg gataggcgcg tggtggacta
    12181 cgccaatgcg actggttgtt aaccccgcgg cgacagcggc aagaatgcca gataagccaa
    12241 aatgttccgc aaccagataa acaataaacg gcaacagaag catcaataca atctgaatgg
    12301 aaggttcatc gtgagcatat cgactgatca acagcagccc tttgccatat aaccaggtaa
    12361 cagcaatccc cacggcaata ccgcccaacg caacgacaaa aaacgcaatg gttgcgccat
    12421 gaacgctgaa ttccatcgtt cccatcgtca ctgcaacagc aaatttcaac gccaccaggc
    12481 cggaagcatc attcatcaac gcttcgcctt cgagcacttc ctggatgttt ttgctgatac
    12541 gccctttccc gacaatcccc agcaacgcca cggcatcggt aggcgacaac acggcggcta
    12601 atgcgaaagc agcgatgagc ggcacattcg gtaagagtaa atgaatcaca tagccgacac
    12661 caaaaatagt gattagcacc aacacaagcg ccaggctgat aatttcccga ctgtgcttga
    12721 tcaattcttt aggttgtatt tttgagcttt cagcaaacaa caaggggggt ataaacaata
    12781 agagaaaaag ttcaggattg aattccacat gcagaccaaa tatgggctgg gccagtacag
    12841 cgccagcggc aatctggatc aaaggaagtg gtatctgaac aggagacaga cgcgtaagca
    12901 cgctggttaa tgatactacc aacgtaagaa ttaaaattgt taaaagtatt tccatataat
    12961 aaacaggctt cctcattcat aaataatggg tattattaat ttaacttcat aaaaagcgcc
    13021 ctggttagca cttaacgtta atcattaatc acagcgttca ctcacatgct caggaggaaa
    13081 ccagtgagat gagttttcca gttgataaca aattcaccat gtgaatattt actaccttta
    13141 accgttgtaa cgaatcatcc gttactcttt gatctccttg ccgaacaagt tatttagtga
    13201 atgatggctt tcttttaacg ttagcatgaa ttatacgttc agcaaatgta ttaaatcagc
    13261 actaaataaa tttaatggat accaccattc tgcgtcaaca cttagttttt atctgtaaaa
    13321 atagccgcag ccaaattaat gcttttattt taataaatca tcaaccgaat aagagatctt
    13381 atcagcggta actaaactca tcagtgggtg ttccttgtaa cgtcatacat tacataactt
    13441 taagatatac aatcaaaaaa tgcattaaaa accgattaaa agcagcaaag tattacccgg
    13501 tgcacgaatt tatgacacat ttgcagccat tccgctattc gctgactact tttcagtaat
    13561 gactgctttt tcataaggtt tcactcccga caacaatgtt ggagttttat ttccttagca
    13621 tggagtaagt aaagatgagc aaaataatag cgatgctaat tactgtcagt accctttttt
    13681 taccgtcaat aatccaggca aaacctattt cgataaaagt tgcctatgaa aataaccctg
    13741 gagaacccct ggatgtggtc atgcgttact gggcagacct attaaataaa aaaagtaatg
    13801 gcgaaataac acttgcgctc tatccaagtt cccaattggg ctcaaaacag gatgtcaccg
    13861 agcaagccat gatgggcatg aatgtcataa ccttgagtga tgtggccttt cttgccgatt
    13921 atgaacccga tttgggtatt ttgtttggac cgtatctgac tgacgatccg caaaaactgt
    13981 tcaaaatata tgaaagcgac tggtttaaac aaaaaaatga aagtctgaag aaaaagggaa
    14041 ttcatgtggt aatgaataat tatctctatg gcactcgaca gattatttcg aaaaaaccca
    14101 ttcgcactgt tgacgatctc gcaggcttaa aaattcgtgt tcctaataac gtcatgcaaa
    14161 taaaagccat tcaggcgatg ggggcaacac caactcccat gccattaggc gaagtttatc
    14221 ccgcattaac ccagggcgtt attgacggcg tagaaaaccc aatttcagtg ctgcaagggc
    14281 aaaaactgta tgagcaggca agatatctga caatggttaa ctacctgaca aatacctcag
    14341 tatggatagg aggtgaagcc ttctttagca ccctttcacc agaacaactg gagatgatcc
    14401 accagaccgg ctatgaagcg ggtgtctata gccagaaact cactcttgaa cgtgatgccg
    14461 aaatgttgaa aaccatgcag gcagcgggcg tcgaagtgat ctatcccgat actggaccat
    14521 tccagaagaa agcacgcgaa gtttactctc aattcccgga atggacacca ggtctgtacg
    14581 aaaccattca acagcaactg caataagcat taatgctcag cgtcctgcct catctctctt
    14641 aggcaggaca cgcactaaca ggaggtttca tgcgtctttt tggcaaagtg gcagaattta
    14701 gtgctgcctt tgcgctattc gttctggttg tgatgacaat tggagcggta tttatgcgtt
    14761 atttcatcgg ccaaccgctt caatggacag aagaaatgtc gggaatgctg atgatttggg
    14821 tggtgatgtt aggtggagtg gtggcggaac gtgacagggc gcacctgacc attcccttcc
    14881 tgatggagat gcttccaggc aagctcaggc gtgttattgc agtactggtc gccgttctct
    14941 ccatcgcact gctgctgtat atggcctggc tgggctaccg actagccgaa atggcgcagt
    15001 ttaaagtgac acaacttctg aaggtttcct ggttctggat cgatctggcg gtccctgtcg
    15061 gtgcactggc aacggctatt tataccctct actggttaat acacgatata aaacaatctg
    15121 atagcggaga caaaatgcaa tgagttggtt attgattatt ccgctaatga tcgtctgttt
    15181 gtttctcaac attcgggtct atctggcgat gtttattggc atcatcgctt actttttatt
    15241 cttctccagc gtaccgattg aaattgccgt gcaaagactg atcgcgccaa cgcaaagccc
    15301 atcattgctg gcaattccgt tttttatctt gctcggcaca ctaatgagct acaccggcat
    15361 tgccgaacgc atcctgcaca ttgccaacgt tctggtgggg aaaatgcgcg gcggacttgg
    15421 cgtggcaaac attctcgtca gcaccatgat gggcggcgtc agcgcctcta accttgctga
    15481 cgcggcgatg ctgtcgcgca tgatggtgcc ggagatggag cgtaaaggct acaaccgcgc
    15541 gtttgccgcc gccattaccg ctggtggctc gttagtcacc ccgatcatac cgccgggaat
    15601 agccctgatt atttacggtc tggttgccga cgtctctatc ggaaaaatgt ttatcgctgg
    15661 gctggtacct ggtttgctat gcgcagtcgt tctgatgttt accgtctacc tggtcgcccg
    15721 caaaactaac gctaaaccct cgcgtgaaag ctggcctacg ggcaaagagg tggtgttctc
    15781 gctgggacag gcctggcctg cgctgttcct catttttgct gtggtgggtg gtattcgcgc
    15841 taacattttc accccaacag aagcaggtgc agcagcggtg ctaatcgtgc tggcgattgg
    15901 cttttttatc tatcgtgaaa tgcggatttc tcatgtcgtc aaagcattgg gcgaaacggc
    15961 acgcgcaacc gcatcggtga tgctggtaat catggccagc gccgcgttgg gatggatttt
    16021 ctcaatggaa catgctggtg tcgcagtggc gaattttgtg acttcgctga ccgaaaataa
    16081 atatatgttc ctgttaatta tcaacatatt gctgctgacg ctggggatgt ttcttgaagg
    16141 aaacgccatt ttgctgatcc tggtaccact attaaaaccg gtggtcgaac actttggcat
    16201 cgatccggtg cattttggca tcatcatgat cttcaacctg tcgattggcg cattcacgcc
    16261 accggtaggt accgtgatgc tgctggtatg taacatcacg caagtttcgg tgggaaattt
    16321 ctttaaacaa tccctgcctc tactggcggc tctactatta atgctaacgc tggtgacata
    16381 tatcccggcg atttcactgt tcctggttta ataataaaaa cgccggatga aatactcatc
    16441 cggcgtcatt gcgttaaaac gtttcccagt tctccccgct atctgccacg gccattttac
    16501 gcggcgcagc tggcgtcacg gtttttacca cagccgatgt ttcacgctgc tgttgctgct
    16561 gttgctgaat ccggaacact gccacagctt cggtcaggcg actggcctgc tcttccagcg
    16621 cggcggcggc ggcggcagat tcttccacca gcgcggcgtt ctgttgagtt acccggtcca
    16681 tctcagcaac cgctaagcca acctgatcga taccacggct ctgctcatca gaagcagaag
    16741 caatttcgcc cataatgtcc gtcacgcggg tcacagcact gacaatctcc gccattgttt
    16801 ccccggcgct ttcgaccagc gtagagccaa catccacttt tcccaccgag tcttcaatca
    16861 ggcttttaat ttcacgagcc gcctgagcgc tacgctgggc cagattacgc acttctcccg
    16921 cgaccaccgc aaaaccgcgt ccttgctcac ccgcacgcgc agcctcaacc gccgcgttca
    16981 aagccagaat attggtctgg aaggcaatgc cgtcaattac gctgataata tcggcgattt
    17041 tctgcgaact ggtggagata tcgcgcatag tctgcaccac gttatctacc actttgccgc
    17101 cgcgttgcgc cgtttcagaa gcacttaatg ccagatggct ggcctggcgc gcattctcgg
    17161 cgttctgttt caccgttgcg gtcagttgct ccatgctggc tgccgtctct tccagcgaag
    17221 cggcctgttg ctcggtgcgc gaagagagat cgttattgcc ggtagcgatt tcgctggcac
    17281 cgctatagat ggcattggcc ccgttgcgca catcaccgac ggtacgcatc agctctccct
    17341 gcatatggcg caaactctct gccagttgcc ccatctcatt agagccatcc acctcaatcg
    17401 gtttcaccag atcgccgcct gcaatatgac gaatgctgtc aatcaggcga ttcattggcg
    17461 ctaccagcga ggctttaata ccgaaccaga cggcgaagat gaccgccagt acgacgatca
    17521 tcacgcccac cagaatccac atcgcctggc tgtaggaggc attgttatcg ctgacggcga
    17581 tatcatagag ccgatcgttt tgctccatgt aagccacata ctgcttctcg aaaccgtcct
    17641 gatatccctg ggtcggctga tcaaagaact cgttgatttt gcctgcacct aatagttgga
    17701 tcagctccgc cagcgcattg tgataaatat cgtaattacg tttgatctct gccgctgcgg
    17761 cggtgctctg acgcgggtca cgcggcaacg cttcgtaatc cgcccagttt ttttccgcct
    17821 gtttcagcga aatactggca ctctgcatca gctcagcaac ggttgaaccg ctaccaatat
    17881 tattctgatc catcatgtag cggatacccg cgcggttgag ggtgttacgc gtctgcaaca
    17941 acgcgaccca gctgccattc agcgtggatt gctgctggcg aatggtttgt aaaacagtga
    18001 aattttcttt gtcattcttt aaggcattaa agaacagacc gcctgatgtc agttgtaaaa
    18061 ggccaaaaac ggccaaaacc agcagtaagc tggtcacaat tttgatacgt tttaacatgg
    18121 tttctctttc ctgtggaccg atgcaagatt ttcggcctgg aaagcaaaaa ctttatgaaa
    18181 aggtgtaacc cgccatccat ggaaaaacca ggcgctggtt atcagatact aaaaagttat
    18241 tcattatcgc ccgggatgtc ttcttgtcgg gaattaccca gagcaataaa ttcctctaac
    18301 agatgggtta actgttgcat tttctcggca gtaaactgag cttcaatctg ccgataggct
    18361 tcttcaatct gtgtctgagc gcggttatac agcgcctgcc cctctttggt cagcgagata
    18421 tagagcttcc gctgatcatt gatcggcttt aatcgcaaca ctaaaccgtc gcgttccatc
    18481 cgcgtgagga tcccggtcag gcttgggcgc aaaatgcagg cgcgatacgc cagatcgtga
    18541 aaatccattg atgggctttc cgccagaata cgcacgatgc gccactgttg ctcagtcaaa
    18601 ttatgccgtt taacgattgg gcgaaaataa ctcatcgccg cttctcgcgc ctggagcaac
    18661 gcaatggtta gagagtcgtg cattatcttt cccctgaaaa attcaaaatc attaatatag
    18721 aaacagttaa tcatcacgtt accgggactg cgctgcccct gcaattcttc attttatcaa
    18781 aattgagcat gttttattaa ctttctgata tttaatattt tattatcttt aaatgtaaat
    18841 agtttgttaa ttagatcaca tttacatcac ttacttttgc caaactacgc aatcgggaat
    18901 aaaagcaatt attaacatat taatgattaa gatgatcgaa tccgaggagt ggtaatgaaa
    18961 ggcactatct tcgccgtagc gttgaaccat cgcagccaac ttgatgcatg gcaggaagcg
    19021 ttccagcaat ccccctacaa agccccgcct aaaactgcgg tctggtttat taaaccgcgc
    19081 aatacggtga ttggttgcgg tgaaccgatt ccctttccac agggtgaaaa ggtactgagc
    19141 ggtgcgactg ttgcgctgat tgtgggaaaa acggcgacga aagtacgtga agaagatgcg
    19201 gcagagtaca tcgccggata tgcgctggct aacgacgtca gcctgccgga agagagcttt
    19261 taccgcccgg caatcaaagc aaaatgtcgt gatggattct gccccattgg cgaaaccgtg
    19321 gctctcagca atgtcgataa tctgaccatc tataccgaga tcaacgggcg tcctgccgat
    19381 cactggaata ccgccgattt acaacgtaac gccgcgcagt tgctgagcgc cctgagcgaa
    19441 tttgccacac tgaatccagg cgatgccatt ctgctcggca cgccacaggc gcgcgtggaa
    19501 atacagccag gcgatcgcgt tcgtgttctc gcagaaggtt tcccgccgct ggaaaatccg
    19561 gtagtggacg aacgtgaagt gaccacgcgc aagagcttcc caacgcagcc acacccgcac
    19621 ggcacgctgt ttgccctcgg cctgaactac gccgaccacg ccagcgaact ggaatttaag
    19681 ccaccggaag aaccgctggt gttcctgaaa gcgccaaata ccctcactgg cgataaccag
    19741 acctccgtgc gtccaaacaa tattgaatac atgcactatg aagcggagct ggtggtagtt
    19801 attggcaaac aggcgcgtaa cgtcagcgaa gccgatgcca tggattatgt cgcgggctac
    19861 accgtgtgta acgactacgc cattcgcgac tatctggaaa actactaccg ccctaacctg
    19921 cgggtaaaaa gccgcgacgg actgacgccg atgctttcaa ccatcgtgcc gaaagaggcg
    19981 atcccggacc cgcataatct gacccttcgc accttcgtca acggcgagtt acgccagcaa
    20041 ggcaccaccg ccgatctgat cttcagcgtg cccttcctga tcgcctactt aagcgaattt
    20101 atgaccctga atccgggcga catgatcgcc accggcacac caaaaggctt atctgacgtg
    20161 gtgcctggcg atgaagtagt ggtggaagta gaaggcgtgg gctgcctggt gaaccgaatt
    20221 gtgagtgagg aaacagcgaa atgaaaaaag taaatcattg gatcaacggc aaaaatgttg
    20281 caggtaacga ctacttccag accaccaatc cggcaacggg tgaagtgctg gcggatgtgg
    20341 cctctggcgg tgaagcggag atcaatcagg cggtagcggc agcgaaagag gcgttcccga
    20401 aatgggccaa tctgccgatg aaagagcgtg cgcgcctgat gcgccgtctg ggcgatctga
    20461 tcgaccagaa cgtgccagag atcgccgcga tggaaaccgc ggacaccggc ctgccgatcc
    20521 atcagaccaa aaatgtgttg atcccacgcg cttcccacaa ctttgaattt ttcgcggaag
    20581 tctgccagca gatgaacggc aagacctatc cggttgacga caagatgctc aactacacgc
    20641 tggtgcagcc ggtgggcgtt tgtgcgctgg tatcgccgtg gaacgtaccg tttatgaccg
    20701 ccacatggaa ggtcgcgccg tgtctggcgc tgggcaatac tgcggtgctg aagatgtcag
    20761 aactctcccc gctgaccgct gaccgcctgg gtgagctggc gctggaagcc ggtattccgg
    20821 cgggcgtgct gaacgtggta cagggctacg gcgcaaccgc aggggatgcg ctggtccgtc
    20881 atcatgacgt gcgtgccgtg tcgttcaccg gcggtaccgc caccgggcgc aacatcatga
    20941 aaaacgccgg gctgaaaaaa tactccatgg aactgggcgg aaaatcgcca gtgctgattt
    21001 ttgaagatgc cgatattgaa cgcgcgctgg acgccgccct gttcaccatc ttctcgatca
    21061 acggcgagcg ctgcaccgcc ggttcgcgca tctttattca gcaaagcatc tacccggaat
    21121 tcgttaaacg ctttgccgaa cgcgccaacc gtctgcgcgt gggcgatccg aacgatccga
    21181 atacccaggt tggggcactt atcagccagc aacactggga aaaagtctcc ggctatatcc
    21241 gtctcggcat tgaagaaggc gcaaccctgc tggcgggcgg cccggataaa ccgtctgacc
    21301 tgcctgcgca cctgaaaggc ggcaacttcc tgcgcccaac ggtgctggca gacgtagata
    21361 accgtatgcg cgtcgcccag gaagagattt tcgggccggt cgcctgcctg ctgccgttta
    21421 aagacgaagc cgaaggctta cgcctggcaa acgacgtgga gtacggcctc gcgtcgtaca
    21481 tctggacaca ggatgtcagc aaagtgttac gcctggcgcg tggcattgaa gcaggcatgg
    21541 tgttcgtcaa tacccagaac gtccgcgacc tgcgccagcc gtttggcggc gtaaaagcct
    21601 ccggcaccgg gcgtgaaggc ggtgagtaca gcttcgaagt gttcgcggaa atgaagaacg
    21661 tctgcatttc catgggcgac catccaattc cgaaatgggg agtctgatat gggtaagtta
    21721 gcgttagccg ccaagatcac gcacgtaccg tcgatgtatc tctctgaact accaggaaaa
    21781 aaccacggtt gtcgccaggg tgcgatcgac gggcataaag agatcagcaa gcgttgccgg
    21841 gaaatgggcg tcgataccat tatcgttttc gacacccact ggctggtcaa cagtgcttat
    21901 cacatcaact gtgcagacca ttttgaaggc gtctacacca gtaacgaact gccgcatttt
    21961 attcgtgaca tgacctacaa ctacgagggc aacccggagt tggggcagct aattgccgat
    22021 gaagccttaa agctcggcgt gcgggcaaaa gcgcacaata ttcccagcct gaaactggaa
    22081 tacggcacgc tggtgccgat gcgctacatg aatgaagaca agcacttcaa agtggtctcc
    22141 atttcggctt tctgcacggt tcacgatttt gccgacagcc gcaagctggg cgaagcgatt
    22201 ctgaaagcga tcgaacagta cgacggcacc gtggcggtcc ttgccagcgg ttcgttatcg
    22261 caccgcttta ttgacgatca gcgtgcggaa gaagggatga acagctacac ccgcgagttc
    22321 gaccgccaga tggacgagcg cgtggtgaag ctgtggcgcg aaggccagtt caaagagttt
    22381 tgcaatatgc tgccggagta cgccgactac tgctacggcg aaggcaatat gcacgacacg
    22441 gtgatgctgc tggggatgct cggctgggat aaatacgacg gcaaggtgga gtttatcacc
    22501 gagctattcc caagctctgg caccggtcag gttaacgctg ttttcccgct tcccgcgtaa
    22561 ggacgtttta tgccgcactt tatcgttgaa tgcagtgata acatccgcga agaagccgac
    22621 ctgccggggt tgttcgccaa agtgaatccg acgctggcag ccacgggtat ttttccgctg
    22681 gcgggtattc gcagccgcgt gcattgggtc gatacctggc agatggccga cgggcagcat
    22741 gattatgcct tcgtgcatat gacgttgaaa atcggcgcag gtcgcagcct ggaaagccgc
    22801 cagcaggcgg gtgaaatgct gtttgaactg attaaaacgc acttcgccgc cctgatggag
    22861 agccgcctgc tggcgttgtc gtttgagatt gaagagctgc atccgacgct gaattttaaa
    22921 caaaacaacg tgcacgcatt gtttaagtag cgcgcagatt gcccggtggc gctgcgctta
    22981 ccgggcctac aaaaccccaa accgtacacc gtaggccgga taaggcgcag ccgcatccgg
    23041 caatgccaca ggatatcgct atgttcgata aacacaccca caccctgatc gcccagcgtc
    23101 tggatcaggc agaaaaacag cgcgaacaga tccgcgcgat ctcgctggat tacccggaga
    23161 tcaccatcga agacgcttac gcggtgcagc gtgaatgggt tcgcctgaaa atcgccgaag
    23221 gtcgcacgct gaaaggccac aaaatcggcc tgacctcgaa agcgatgcag gcaagctcgc
    23281 agatcagcga accggattac ggcgcgctgc tggatgacat gttcttccac gatggcagcg
    23341 atatcccgac cgatcgcttt atcgtgccgc gcattgaagt ggagctggct ttcgtgctgg
    23401 caaaaccgct gcgtgggcca aactgcacgc tgttcgacgt ctacaacgcc acggactacg
    23461 tgatcccggc gctggagctg atcgacgctc gctgccacaa catcgatccg gaaacccagc
    23521 gcccgcgcaa agttttcgac accatttctg ataacgccgc caacgccggg gtgatcctcg
    23581 gaggtcgtcc gatcaaacca gacgaactgg atctgcgttg gatctccgcc ctgatgtatc
    23641 gcaatggcgt gattgaagaa accggcgtcg ccgctggcgt gctgaatcat ccggcaaacg
    23701 gcgtggcctg gctggcgaac aaactcgcac cgtatgacgt acaactggaa gccgggcaaa
    23761 tcattctcgg tggttcgttc acccgcccgg ttccggcgcg taagggcgac accttccacg
    23821 tcgattacgg caacatgggt tccattagct gccgctttgt ttaaggagag aacgatggaa
    23881 aacagcttta aagcggcgct gaaagcaggc cgtccgcaga tcggattatg gctggggctg
    23941 agtagcagct acagcgcaga gttactggcc ggagcaggat tcgactggtt gttgatcgac
    24001 ggtgagcacg cgccgaataa cgtgcaaacc gtgctcaccc agctacaggc gattgcgccc
    24061 taccccagcc agccggtagt acgtccgtcg tggaacgatc cggtgcaaat caaacaactg
    24121 ctggacgtcg gcacacaaac cttgctggtg ccgatggtac aaaacgctga cgaagcccgt
    24181 gaagcggtac gcgccacccg ttatcccccc gccggtattc gcggtgtggg cagtgcgctg
    24241 gcccgagcct cgcgctggaa tcgcattcct gattacctgc aaaaagccaa cgatcaaatg
    24301 tgcgtgctgg tgcagatcga aacgcgtgag gcaatgaaga acttaccgca gattctggac
    24361 gtggaagggg tcgacggcgt gtttatcggc ccggcggatc tgagcgccga tatgggttat
    24421 gccggtaatc ctcagcaccc ggaagtacag gccgcgattg agcaggcgat cgtgcagatc
    24481 cgcgaagcgg gcaaagcgcc ggggatcctg atcgccaatg agcaactggc aaaacgctat
    24541 ctggaactgg gcgcgctgtt tgtcgccgtc ggcgttgaca ccaccctgct cgcccgcgcc
    24601 gccgaagcgc tggcagcacg gtttggtgcg caggctacag cgattaagcc cgacgtgtat
    24661 taatgccgga tgcgctgcgc ttatccggcc tacactcgca ccaaaccgta ggccggataa
    24721 ggcgtttacg ccgcatccgg caaaaagccg taccaaatcg cggaccggat aaggcgtttg
    24781 tgcgcatccg ccataaacct tgtcgtaccc tacaaaaatc ccattagagg aagtaaaatg
    24841 agcgacacct cacctgccat accggagagt atcgatccgg cgaatcagca taaagcgctg
    24901 actgccggac aacaggcggt tattaagaag ctatttcgcc gcctgatcgt ctttctgttc
    24961 gtgctgttta tcttctcgtt ccttgatcgc atcaacatcg gctttgccgg actcacgatg
    25021 ggacgcgacc tcggtctgag cgccaccatg tttggcctcg ctaccacctt gttctacgcc
    25081 gcttacgtca tcttcggcat tcccagcaac attatgctga gtattgtcgg tgcacggcgc
    25141 tggatcgcca ccatcatggt gctttggggc atcgcctcta ctgccaccat gtttgccact
    25201 ggccccacca gcttatacgt actgcgtata ctggttggca ttaccgaagc cggctttctg
    25261 cctggcattc tgctgtattt aaccttctgg tttccggcct atttccgcgc ccgtgccaac
    25321 gccttgttta tggtagcaat gccggtaacg acagcgttgg gatcgatcgt ttccggctac
    25381 attttgtcgc tggatggcgt aatggcatta aaaggctggc agtggctgtt tttgctggaa
    25441 ggcttcccgt cggtattact cggcgtcatg gtgtggttct ggcttgatga ctcaccggac
    25501 aaagctaagt ggctgacgaa agaagacaaa aaatgcctgc aagagatgat ggataacgat
    25561 cgtctgacgc tggttcagcc agagggagcc atcagccacc acgccatgca acaacgcagt
    25621 atgtggcggg agatcttcac tccggtggtg atgatgtata ccctggcgta tttctgcctg
    25681 accaacacac ttagtgcgat cagcatctgg acaccgcaga tcctgcaaag ctttaatcag
    25741 ggcagcagta atatcaccat cggcctgctg gccgccgtac cgcagatttg taccattctc
    25801 gggatgatct actggagccg tcactcagat cgccgccagg aacgaaggca tcacaccgcc
    25861 cttccttatt tgttcgctgc cgctggttgg ttactggctt cggcaactga tcacaacatg
    25921 atccagatgc tggggatcat tatggcttcg accggatcat tcagcgcaat ggcgattttc
    25981 tggacaacac cggatcagtc catcagcctg cgggcacgag cgatcggtat tgcggtgatc
    26041 aacgccactg gcaacattgg ttcagcgtta agtccgttta tgatcggctg gttgaaagat
    26101 ctgaccggca gctttaacag tggattgtgg tttgttgcca cgctgctggt gattggtgcg
    26161 gggattatct gggcgattcc aatgcagtcc tcccgtccgc gagcgacccc gtaaggaacg
    26221 acgatgtgtg accgtcagat tgccaatatt gatatcagca aagagtacga tgaaagcctg
    26281 ggcacggacg atgtgcatta tcagtcgttc gcccgcatgg cggccttttt tggccgccat
    26341 atgctgccac atcgccacga acagtacttt cagatgcatt tcctcaatag cggacagatt
    26401 gagctacagc ttgacgatca tcgctactcg gtggaagcgc ccctgtttgt cctgacgccg
    26461 ccgtcagtac ctcatgcgtt tattacggag tctgatgctg acggtcatgt attgacggta
    26521 cgggaagatc tgatctggcc cctgctggaa gttctttatc cgggcactcg ggaaaccttc
    26581 ggcctgccgg ggatttgcct gtcactggca gataaacccg acgaactggc ggcgctggaa
    26641 cactattggc aactgataga gcgggaatcg gtagaacaac tgcctggtcg ggaacacacc
    26701 ctgacgttac tggcacaggc agtgttcacc ctactgctgc gtaacgcaaa actcgacgac
    26761 catgccgcca gcggaatgcg cggagaatta aaactgttcc agcgttttca tatgctgatt
    26821 gaaagccatt ttcatcagca ctggacagta ccggattacg ctaacgaatt gcatatcacc
    26881 gaatcacgcc tcactgacat ctgccgccgc tttgccaacc gtccgccaaa acggttgatt
    26941 ttcgacaggc agctgcgaga agccaagcgg ctgctgctgt tttctgataa cgccgtgaac
    27001 aatattgcct ggcaactcgg ttttaaggat ccggcttatt ttgcgcgctt ttttaatcgc
    27061 ttagtcggtt gctcgcccag tgcttatcgc gccaaaaaag tacctgtgac gtgaaaatcc
    27121 cttagcctta acgggaaacc aggcaccacc tgctattccc ctttctccgc ctggagcaag
    27181 gggatttagt aacgttaaaa atcgctaaaa gcgacctcga tcacaaaacc gtacccgatc
    27241 tgaaaagtac cagccattgt cccaaaagtg tcttcccgta atcgcccctg caagcttcaa
    27301 ttgaataaca aaaacaacac acaaaattaa caatcgtatt ccgattaata ctgtagaggt
    27361 cgacatgaaa ccagaagatt tccgcgccag tacccaacgt ccgttcaccg gggaagagta
    27421 tctgaaaagc ctgcaggatg gtcgcgagat ctatatctat ggcgagcgag tgaaagacgt
    27481 caccactcat ccggcatttc gtaatgcggc agcgtctgtt gcccaactgt acgacgcgct
    27541 acacaaaccg gagatgcagg actctctgtg ctggaacacc gacaccggta gcggcggcta
    27601 tacccataaa ttcttccgcg tggcgaaaag tgccgacgac ctgcgccagc aacgcgacgc
    27661 catcgctgag tggtcacgcc tgagctatgg ctggatgggc cgtaccccag actacaaagc
    27721 cgctttcggt tgcgcactgg gcgcgaatcc gggcttttac ggtcagttcg agcagaacgc
    27781 ccgtaactgg tatacccgta ttcaggaaac tggcctctac tttaaccacg cgattgttaa
    27841 cccaccgatc gatcgtcatt tgccgaccga taaagtgaaa gacgtttaca tcaagctgga
    27901 aaaagagact gacgccggga ttatcgtcag cggtgcgaaa gtggttgcca ccaactcggc
    27961 gctgactcac tacaacatga ttggcttcgg ctcggcacaa gtgatgggcg aaaacccgga
    28021 cttcgcactg atgttcgttg cgccaatgga tgccgatggc gtgaaattaa tctcccgcgc
    28081 ctcttatgag atggtcgcgg gtgctaccgg ctcgccgtac gactacccgc tctccagccg
    28141 ctttgatgag aatgatgcga ttctggtgat ggataacgtg ctgatcccat gggaaaacgt
    28201 actgatctac cgcgatttcg atcgctgccg tcgctggacg atggaaggcg gttttgcccg
    28261 tatgtatccg ctgcaagcct gtgtgcgcct ggcagtgaaa ctagacttca ttacggcact
    28321 gctgaaaaaa tcactcgaat gtaccggcac cctggagttc cgtggtgtgc aggccgatct
    28381 cggtgaagtg gtggcgtggc gcaacacctt ctgggcattg agtgactcga tgtgttcaga
    28441 agcgacacca tgggtcaacg gtgcttattt accggatcat gccgcactgc aaacctatcg
    28501 cgtactggca ccaatggcct acgcgaagat caaaaacatt atcgaacgca acgttaccag
    28561 tggcctgatc tatctccctt ccagtgcccg tgacctgaac aatccgcaga tcgaccagta
    28621 tctggcgaag tatgtgcgcg gttcgaacgg tatggatcac gtccagcgca tcaagatcct
    28681 caaactgatg tgggacgcca ttggcagcga gtttggtggt cgtcacgaac tgtatgaaat
    28741 caactactcc ggtagccagg atgagattcg cctgcagtgt ctgcgccagg cacaaagctc
    28801 cggcaatatg gacaagatga tggcgatggt tgatcgctgc ctgtcggaat acgaccagaa
    28861 cggctggact gtgccgcacc tgcacaacaa cgacgatatc aacatgctgg ataagctgct
    28921 gaaataacgc agcaggaggt taagatgcaa ttagatgaac aacgcctgcg ctttcgtgac
    28981 gcaatggcca gcctgtcggc agcggtaaat attatcacca ccgagggcga cgccggacaa
    29041 tgcggaatta cggcaacggc cgtctgctcg atcacggata caccaccgtc gctgatggtg
    29101 tgcattaacg ccaacagtgc gatgaacccg gtttttcagg gcaacggtaa gttgtgcgtc
    29161 aacgtcctca atcatgagca ggagctgatg gcccgccact tcgcgggcat gacaggcatg
    29221 gcgatggaag agcgttttag cctctcatgc tggcaaaaag gtccgctggc acagccggtg
    29281 ctgaaaggtt cgctggccag tcttgaaggt gagatccgcg atgtgcaggc aattggcaca
    29341 catctggtgt atctggtgga gattaaaaac atcatcctca gtgcagaagg tcacggactt
    29401 atctacttta aacgccgttt ccatccggtg atgctggaaa tggaagctgc gatttaagct
    29461 ccagcccctt cacgcattcg tcgaaggggc atttttattg tgatctcaat cacatatcca
    29521 cccgatgaat aattcttacc caaccgctca ccgccattcc tgaccgctta catccctaaa
    29581 ataaccactc agttatttac cttactttac gcgtgcgtaa ctctggcaac atcactgcag
    29641 gatagcggtc aatttacctc ctcaaacgca acgcaaacct agaacggctt cggcctatga
    29701 ttaacaaatt actctatgga ttttggtttg caggaaggca gcaagtgagt gaatccccgg
    29761 gagcttataa cagtaagtga caggggtgaa cgaacgcagc tgccgcacct gtaagccaaa
    29821 agacgacgag taaaatgcca ggttttacta tggatacgaa aaaactattc aagcacatac
    29881 cctgggtgat tctcggaatc atcggtgcat tctgcctcgc ggtagttgca ttacgtcggg
    29941 gggagcacgt cagcgccctg tggatcgtgg tcgcctctgt gtcggtgtat ctggtggcgt
    30001 atcgctacta cagtctgtat atcgcccaga aggtgatgaa actcgacccc acgcgcgcga
    30061 cgcctgcggt tattaacaac gacggtctga actacgttcc gaccaaccgt tacgtgttgt
    30121 ttggtcacca cttcgccgct atcgccggtg ctggtccgct ggttggtccg gttctcgccg
    30181 cgcagatggg ctacctgccc ggcacgctgt ggctgctggc gggggtagtg ctggccggtg
    30241 cggtacagga ctttatggtg ctgtttatct cctctcgccg taacggcgca tctctcggtg
    30301 agatgatcaa agaagagatg ggaccagtac cggggactat cgcgctgttt ggctgtttct
    30361 taatcatgat catcatcctt gccgtcctgg cgctgattgt ggttaaagcc ctggcagaaa
    30421 gtccgtgggg tgtcttcacc gtttgctcaa ccgtaccgat tgcgctgttt atgggtatct
    30481 acatgcgctt tatccgtccg gggcgtgtgg gtgaagtctc tgtaatcggt atcgtgctgc
    30541 tggttgcctc tatctacttc ggtggcgtga ttgctcacga tccgtactgg ggtccggcac
    30601 tgacctttaa agacaccacc attaccttcg cgctgattgg ctatgcgttt gtttccgcac
    30661 tgctgccggt gtggctgatc ctcgcaccgc gcgactatct ggcaaccttc ctgaaaatcg
    30721 gcgttatcgt cggcctggcg ttgggtatcg tggtgctgaa cccggaactg aaaatgcctg
    30781 cgatgaccca gtacattgac ggtactggcc cgctgtggaa aggcgctctg ttcccgttcc
    30841 tgttcatcac catcgcctgt ggtgcggtat ctggcttcca cgcgctgatc tcttccggta
    30901 cgacgccgaa actgctggct aacgaaaccg acgcgcgttt cattggctac ggcgcaatgc
    30961 tgatggagtc cttcgtggcg attatggcgc tggttgcagc gtccatcatc gaaccgggtc
    31021 tttacttcgc gatgaacacc ccgcctgcgg gccttggcat caccatgcct aacctgcatg
    31081 aaatgggtgg cgagaacgcg ccgatcatca tggcgcagct gaaagacgtt accgcacacg
    31141 cggcggcgac cgtcagctcc tggggcttcg tgatttcgcc agagcagatc ctgcaaaccg
    31201 cgaaagacat tggtgagcct tctgtcctga accgtgcagg tggtgcgccg acactggcgg
    31261 taggtatcgc gcacgtgttc cacaaagtgc tgccgatggc tgacatgggc ttctggtatc
    31321 acttcggtat tctgttcgaa gccctgttca tcctgaccgc gctggatgcg ggtacccgtt
    31381 ctggccgctt tatgctgcag gacctgctgg gtaacttcat cccgttcctg aaaaaaaccg
    31441 actctctggt tgctggtatc atcggtactg cgggctgtgt gggtctgtgg ggctacctgc
    31501 tgtatcaggg cgtggtcgat ccgctgggcg gcgttaagag cctgtggccg ctgttcggta
    31561 tctccaacca gatgctggca gccgtagcgc tggtactggg caccgttgtg ctgattaaga
    31621 tgaagcgcac ccaatacatc tgggtaactg ttgttccggc tgtatggctg cttatctgca
    31681 ccacctgggc gctgggcctg aaactgttca gcaccaaccc gcagatggaa ggcttcttct
    31741 acatggcaag ccagtacaaa gagaagattg ctaacggtac tgacctgacg gcacagcaga
    31801 ttgccaacat gaaccacatc gttgtgaaca actacaccaa cgcgggcctg agtattctgt
    31861 tcctgattgt ggtgtacagc atcatcttct acggtttcaa aacctggctt gcggtgcgta
    31921 acagcgacaa acgtactgac aaagaaacac cgtacgttcc aatcccggaa ggcggcgtga
    31981 agatctcttc gcaccactaa ccggttgccg gatgcgttgc gcgtatccgg cctacaaagt
    32041 gtctatcgta ggccggataa agcgtttacg ccgcatccgg caaatcccta gcccggacct
    32101 tccgggcttt acgcatatct ggaaggcaaa atgtttggta acttaggaca ggcaaaaaaa
    32161 tatctcggcc aggcggcgaa gatgttgatt ggcattccag actacgacaa ctacgtcgag
    32221 catatgaaga ccaaccatcc cgacaagccg tacatgagct atgaagaatt cttccgcgaa
    32281 cgccagaacg cgcgctacgg cggcgatggt aaaggcggta tgcgctgttg ttaatggaga
    32341 gagcatgaac ccgattgcag ttaccctact caccggtttt ttaggcgcgg gcaaaaccac
    32401 cctgctgcgc catattctta acgaacaaca cggctacaag attgccgtga ttgaaaacga
    32461 attcggcgaa gtctccgttg atgatcaact gattggcgac cgcgccacgc agatcaaaac
    32521 gctgaccaac ggctgcatct gctgttcgcg ctccaacgag ctggaagacg cactactcga
    32581 cctgctggat aatctcgaca agggcaatat tcagttcgac cgtctggtca ttgaatgcac
    32641 cggcatggcc gatcccggtc cgattattca gacctttttc tcccatgaga ttttatgcca
    32701 gcgttacctg ctggacggcg tgattgcgct ggtggatgcg gtacatgccg atgagcagat
    32761 gaaccagttc accatcgccc agtcccaggt tggctacgcc gaccgtattc tgctgaccaa
    32821 aaccgacgtc gcaggcgaag cagaaaaact gcgtgaacgc ctggcgcgca tcaacgcccg
    32881 tgcaccggtc tacaccgtca cccacggcga catcgacctg ggcctgctat tcaacaccaa
    32941 cggttttatg ctggaagaga acgtcgtcag caccaaaccg cgcttccact ttatcgcgga
    33001 taaacaaaac gatatttcgt cgattgtggt ggaactggat tacccggtag atatcagcga
    33061 agtttcccgg gtgatggaaa acctgctgct ggaatcggca gataaactgc tgcgttacaa
    33121 agggatgctg tggattgacg gcgaacctaa ccgcctgcta ttccagggcg tccagcgcct
    33181 ctacagcgcc gactgggacc gcccgtgggg cgatgaaaaa ccgcatagca cgatggtgtt
    33241 tatcgggatt cagctgccgg aggaagagat tcgggcggcg tttgcggggt tgaggaagta
    33301 atcgtgaaag gttgagtgta aagcagctca atgccccatt tagcagtgag gggggaaaca
    33361 gaacgacgtg ctcccctccg ctaagtttta ttgcgcgtga atacctggat ctggcaaaaa
    33421 attgccgcgt ttggcgtcag accatgcttc gctaccagaa gcaggaatgt cgctgtaatc
    33481 aatgtcttcg tcggatttat taaccaaagc catgagttca gcttcttgct gcacatcaag
    33541 tgtgtagagt gtgccccgtt tatgtttaac catgctcatt tatgaccaga acctttacga
    33601 tttaattata aagatgactt atttcagcca tccaggcacg ttatattctg agacattctc
    33661 aatggaatat gcatctctcc tgtcagaacc tttcttacac ttcaactcat tgacccccca
    33721 tttaccc
//
LOCUS       DAZFWL020000041        32775 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFWL020000041 DAZFWL020000000
VERSION     DAZFWL020000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.041"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..282)
                     /gene="tccP2"
                     /locus_tag="ACHKNP_RS10370"
                     /old_locus_tag="pgaptmp_002074"
     CDS             complement(<1..282)
                     /gene="tccP2"
                     /locus_tag="ACHKNP_RS10370"
                     /old_locus_tag="pgaptmp_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HGU4704243.1"
                     /translation="MINSINSFFSSIPHSISSVTRNSSFTASQHKSTPNTVKTSSPLS
                     PSNSPASATTIFKVKNSYTESGLQRSTSYTQSSIEKNALHRPLPDVAKRL"
     gene            complement(728..1132)
                     /locus_tag="ACHKNP_RS10375"
                     /old_locus_tag="pgaptmp_002075"
                     /pseudo
     CDS             complement(728..1132)
                     /locus_tag="ACHKNP_RS10375"
                     /old_locus_tag="pgaptmp_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
     gene            complement(1215..1302)
                     /locus_tag="ACHKNP_RS10380"
                     /old_locus_tag="pgaptmp_002076"
     tRNA            complement(1215..1302)
                     /locus_tag="ACHKNP_RS10380"
                     /old_locus_tag="pgaptmp_002076"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1266..1268),aa:Ser,seq:tga)
     gene            1729..2847
                     /gene="hyaA"
                     /locus_tag="ACHKNP_RS10385"
                     /old_locus_tag="pgaptmp_002077"
     CDS             1729..2847
                     /gene="hyaA"
                     /locus_tag="ACHKNP_RS10385"
                     /old_locus_tag="pgaptmp_002077"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HGU4704244.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2844..4637
                     /gene="hyaB"
                     /locus_tag="ACHKNP_RS10390"
                     /old_locus_tag="pgaptmp_002078"
     CDS             2844..4637
                     /gene="hyaB"
                     /locus_tag="ACHKNP_RS10390"
                     /old_locus_tag="pgaptmp_002078"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HGU4704245.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            4656..5363
                     /gene="hyaC"
                     /locus_tag="ACHKNP_RS10395"
                     /old_locus_tag="pgaptmp_002079"
     CDS             4656..5363
                     /gene="hyaC"
                     /locus_tag="ACHKNP_RS10395"
                     /old_locus_tag="pgaptmp_002079"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HGU4704246.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWMGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5360..5947
                     /gene="hyaD"
                     /locus_tag="ACHKNP_RS10400"
                     /old_locus_tag="pgaptmp_002080"
     CDS             5360..5947
                     /gene="hyaD"
                     /locus_tag="ACHKNP_RS10400"
                     /old_locus_tag="pgaptmp_002080"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HGU4704247.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            5944..6342
                     /gene="hyaE"
                     /locus_tag="ACHKNP_RS10405"
                     /old_locus_tag="pgaptmp_002081"
     CDS             5944..6342
                     /gene="hyaE"
                     /locus_tag="ACHKNP_RS10405"
                     /old_locus_tag="pgaptmp_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HGU4704248.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVLLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            6339..7196
                     /gene="hyaF"
                     /locus_tag="ACHKNP_RS10410"
                     /old_locus_tag="pgaptmp_002082"
     CDS             6339..7196
                     /gene="hyaF"
                     /locus_tag="ACHKNP_RS10410"
                     /old_locus_tag="pgaptmp_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HGU4704249.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            7330..8875
                     /gene="appC"
                     /locus_tag="ACHKNP_RS10415"
                     /old_locus_tag="pgaptmp_002083"
                     /pseudo
     CDS             7330..8875
                     /gene="appC"
                     /locus_tag="ACHKNP_RS10415"
                     /old_locus_tag="pgaptmp_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
     gene            8887..10023
                     /gene="appB"
                     /locus_tag="ACHKNP_RS10420"
                     /old_locus_tag="pgaptmp_002084"
     CDS             8887..10023
                     /gene="appB"
                     /locus_tag="ACHKNP_RS10420"
                     /old_locus_tag="pgaptmp_002084"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU4704250.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPSAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            10036..10128
                     /gene="cbdX"
                     /locus_tag="ACHKNP_RS10425"
                     /old_locus_tag="pgaptmp_002085"
     CDS             10036..10128
                     /gene="cbdX"
                     /locus_tag="ACHKNP_RS10425"
                     /old_locus_tag="pgaptmp_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HGU4704251.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            10208..11506
                     /gene="appA"
                     /locus_tag="ACHKNP_RS10430"
                     /old_locus_tag="pgaptmp_002086"
     CDS             10208..11506
                     /gene="appA"
                     /locus_tag="ACHKNP_RS10430"
                     /old_locus_tag="pgaptmp_002086"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HGU4704252.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11621..13801)
                     /gene="etk"
                     /locus_tag="ACHKNP_RS10435"
                     /old_locus_tag="pgaptmp_002087"
     CDS             complement(11621..13801)
                     /gene="etk"
                     /locus_tag="ACHKNP_RS10435"
                     /old_locus_tag="pgaptmp_002087"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HGU4704253.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQAQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVAHFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13821..14267)
                     /gene="etp"
                     /locus_tag="ACHKNP_RS10440"
                     /old_locus_tag="pgaptmp_002088"
                     /gene_synonym="yccY"
     CDS             complement(13821..14267)
                     /gene="etp"
                     /locus_tag="ACHKNP_RS10440"
                     /old_locus_tag="pgaptmp_002088"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HGU4704254.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(14255..15394)
                     /gene="gfcE"
                     /locus_tag="ACHKNP_RS10445"
                     /old_locus_tag="pgaptmp_002089"
     CDS             complement(14255..15394)
                     /gene="gfcE"
                     /locus_tag="ACHKNP_RS10445"
                     /old_locus_tag="pgaptmp_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HGU4704255.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(15440..17536)
                     /gene="gfcD"
                     /locus_tag="ACHKNP_RS10450"
                     /old_locus_tag="pgaptmp_002090"
     CDS             complement(15440..17536)
                     /gene="gfcD"
                     /locus_tag="ACHKNP_RS10450"
                     /old_locus_tag="pgaptmp_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU4704256.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(17536..18282)
                     /gene="gfcC"
                     /locus_tag="ACHKNP_RS10455"
                     /old_locus_tag="pgaptmp_002091"
     CDS             complement(17536..18282)
                     /gene="gfcC"
                     /locus_tag="ACHKNP_RS10455"
                     /old_locus_tag="pgaptmp_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU4704257.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(18279..18923)
                     /gene="gfcB"
                     /locus_tag="ACHKNP_RS10460"
                     /old_locus_tag="pgaptmp_002092"
     CDS             complement(18279..18923)
                     /gene="gfcB"
                     /locus_tag="ACHKNP_RS10460"
                     /old_locus_tag="pgaptmp_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HGU4704258.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(19030..19335)
                     /gene="gfcA"
                     /locus_tag="ACHKNP_RS10465"
                     /old_locus_tag="pgaptmp_002093"
     CDS             complement(19030..19335)
                     /gene="gfcA"
                     /locus_tag="ACHKNP_RS10465"
                     /old_locus_tag="pgaptmp_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HGU4704259.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(19777..19989)
                     /gene="cspH"
                     /locus_tag="ACHKNP_RS10470"
                     /old_locus_tag="pgaptmp_002094"
     CDS             complement(19777..19989)
                     /gene="cspH"
                     /locus_tag="ACHKNP_RS10470"
                     /old_locus_tag="pgaptmp_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HGU4704260.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            20275..20487
                     /gene="cspG"
                     /locus_tag="ACHKNP_RS10475"
                     /old_locus_tag="pgaptmp_002095"
     CDS             20275..20487
                     /gene="cspG"
                     /locus_tag="ACHKNP_RS10475"
                     /old_locus_tag="pgaptmp_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HGU4704261.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            20498..20686
                     /gene="ymcF"
                     /locus_tag="ACHKNP_RS10480"
                     /old_locus_tag="pgaptmp_002096"
     CDS             20498..20686
                     /gene="ymcF"
                     /locus_tag="ACHKNP_RS10480"
                     /old_locus_tag="pgaptmp_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU4704262.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            20661..20891
                     /gene="ymcE"
                     /locus_tag="ACHKNP_RS10485"
                     /old_locus_tag="pgaptmp_002097"
     CDS             20661..20891
                     /gene="ymcE"
                     /locus_tag="ACHKNP_RS10485"
                     /old_locus_tag="pgaptmp_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HGU4704263.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            20881..21054
                     /gene="gnsA"
                     /locus_tag="ACHKNP_RS10490"
                     /old_locus_tag="pgaptmp_002098"
     CDS             20881..21054
                     /gene="gnsA"
                     /locus_tag="ACHKNP_RS10490"
                     /old_locus_tag="pgaptmp_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGU4704264.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(21102..22175)
                     /gene="yccM"
                     /locus_tag="ACHKNP_RS10495"
                     /old_locus_tag="pgaptmp_002099"
     CDS             complement(21102..22175)
                     /gene="yccM"
                     /locus_tag="ACHKNP_RS10495"
                     /old_locus_tag="pgaptmp_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU4704265.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(22247..24991)
                     /gene="torS"
                     /locus_tag="ACHKNP_RS10500"
                     /old_locus_tag="pgaptmp_002100"
     CDS             complement(22247..24991)
                     /gene="torS"
                     /locus_tag="ACHKNP_RS10500"
                     /old_locus_tag="pgaptmp_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HGU4704266.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISTHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            25074..26102
                     /gene="torT"
                     /locus_tag="ACHKNP_RS10505"
                     /old_locus_tag="pgaptmp_002101"
     CDS             25074..26102
                     /gene="torT"
                     /locus_tag="ACHKNP_RS10505"
                     /old_locus_tag="pgaptmp_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HGU4704267.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(26075..26767)
                     /gene="torR"
                     /locus_tag="ACHKNP_RS10510"
                     /old_locus_tag="pgaptmp_002102"
     CDS             complement(26075..26767)
                     /gene="torR"
                     /locus_tag="ACHKNP_RS10510"
                     /old_locus_tag="pgaptmp_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HGU4704268.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            26897..28069
                     /gene="torC"
                     /locus_tag="ACHKNP_RS10515"
                     /old_locus_tag="pgaptmp_002103"
     CDS             26897..28069
                     /gene="torC"
                     /locus_tag="ACHKNP_RS10515"
                     /old_locus_tag="pgaptmp_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HGU4704269.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            28069..30615
                     /gene="torA"
                     /locus_tag="ACHKNP_RS10520"
                     /old_locus_tag="pgaptmp_002104"
     CDS             28069..30615
                     /gene="torA"
                     /locus_tag="ACHKNP_RS10520"
                     /old_locus_tag="pgaptmp_002104"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HGU4704270.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            30612..31211
                     /gene="torD"
                     /locus_tag="ACHKNP_RS10525"
                     /old_locus_tag="pgaptmp_002105"
     CDS             30612..31211
                     /gene="torD"
                     /locus_tag="ACHKNP_RS10525"
                     /old_locus_tag="pgaptmp_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HGU4704271.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVGRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(31304..31609)
                     /gene="cbpM"
                     /locus_tag="ACHKNP_RS10530"
                     /old_locus_tag="pgaptmp_002106"
     CDS             complement(31304..31609)
                     /gene="cbpM"
                     /locus_tag="ACHKNP_RS10530"
                     /old_locus_tag="pgaptmp_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HGU4704272.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(31609..32529)
                     /gene="cbpA"
                     /locus_tag="ACHKNP_RS10535"
                     /old_locus_tag="pgaptmp_002107"
     CDS             complement(31609..32529)
                     /gene="cbpA"
                     /locus_tag="ACHKNP_RS10535"
                     /old_locus_tag="pgaptmp_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HGU4704273.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         7780 a         8311 c         8605 g         8079 t
ORIGIN      
        1 taggcgtttg gccacatcag gtagaggccg atgtaaagcg tttttttcta tacttgactg
       61 agtataagat gtggaacgtt gaagtcctga ttccgtataa gaatttttta ccttaaaaat
      121 agttgttgca ctggcaggac tgttgctggg agaaagaggt gagctggttt ttaccgtgtt
      181 aggtgtgctt ttgtgctgtg aggcagtgaa gctactgttg cgcgtaacac ttgatatact
      241 gtgaggaatg ctggaaaaaa aagaattaat gctatttatc atatagataa ccttataaga
      301 actttatgtg atagtctaat ttatccgttt gcatgaaatc taccttttgt gaaattctcg
      361 tcaatatttt actcaaaatg atcggctgta ggaataaact atttgatatt attgcgtttt
      421 tagagttgtt ttcaatcagc tatagatagg gcttggcgcc agaatggaga atatttaacg
      481 agaaatagaa gctttgtatc agacgggctt ccctgaatag agaagttaaa taatcaggat
      541 gatgctaatg aatatcagga acaaggtgag tgtcttcatg ttcactgaaa cagggtgtca
      601 caaaaaatat ggctataaga tgatcaatgt gatttgaaat atttataggt ttacaggggg
      661 ctgggcatgc atatcttact gcaatgcaga atatgtgctg cttggcgcag ctctttattg
      721 agaatgttca atcttttgtt tcagtttcgt gaaggatacg gacggtttct tgtgtaatat
      781 tatacatcgc ctgtttatct ttactgcata ctcctgcaag tgttaatgct actccagcat
      841 aaatttctgg ataactatgt gtaaatatat ccagtgagtt tacatgtttc tctttcattc
      901 ctgcatcaca acgaacctta tttatacctg tcgccattga ttggggaggg ctagagcctt
      961 ctgcacgaaa atagtgatgt gacagatctg agatagcttt gtatccttct gcctggttca
     1021 ggcctggagc agtccatgtt ttacaaaaat actcggcagc gtaacgtgtt gcaagagcgg
     1081 catcagacga ggaacctgag gttccgctca tgaccgataa tctcatccga ataaaaatat
     1141 accaattggt ataccaagtt tataccaaga gccaaaaaac aaaggggcta ccatttggta
     1201 accccttgat tatttggcgg aagcgcagag attcgaactc tggaaccctt tcgggtcgcc
     1261 ggttttcaag accggtgcct tcaaccgctc ggccacactt ccggaatgac gcgcactata
     1321 aacatcccga tgcggcgtgt aaacccctaa tttgtttgtt tgcctgaaaa acagccaaaa
     1381 gtgcattgat agcgtgaaat aacagcagat tgatcatttc atcaccatga attccttctc
     1441 ttttactcgt ttagcaaccg gctaaacatc cccaccgccc ggccaaaaga aaaataggtc
     1501 catttttatc gctaaaagat aaatccacac agtttgtatt gttttgtgca aaagtttcac
     1561 tacgctttat taacaatact ttctggcgac gtgtgccagt gcagaaggat gagctttcgt
     1621 tttcagcatc tcacgtgaag cgatggtttg ccttgctaca gggacgtcgc ttgccgacca
     1681 taagcgcccg gtgtcctgcc ggtgtcgcaa ggagaagaga cgtgcgatat gaataacgag
     1741 gaaacatttt accaggccat gcggcgtcag ggcgttaccc ggcgcagctt tctcaaatat
     1801 tgtagtctgg ctgccacgtc gctgggatta ggcgcgggaa tggcaccaaa gattgcctgg
     1861 gcgctggaga acaaaccccg cattccggtg gtatggatcc acggtctgga atgcacctgc
     1921 tgtaccgaat cttttatccg ctccgctcac ccactggcga aggacgtcat cctttccctg
     1981 atttccctcg attatgacga tactttgatg gctgccgccg gaacccaggc ggaagaagtc
     2041 ttcgaagaca tcatcacgca atacaatggc aaatatatcc tcgcagtaga aggtaatccg
     2101 ccgctgggcg agcaggggat gttctgtatc agcagcggtc gaccgtttat tgagaaactc
     2161 aaacgtgccg ctgccggagc cagcgcgatt atcgcctggg gaacctgcgc gtcctggggc
     2221 tgcgtgcagg ccgcgcggcc caatccgacg caggcaacgc ctatcgacaa agtcatcacc
     2281 gacaaaccca ttatcaaagt acctggctgc ccgccgatcc cggatgtgat gagcgccatc
     2341 attacttaca tggtgacctt tgatcgcttg ccagatgtcg acagaatggg ccgtccgctg
     2401 atgttctatg gtcagcgaat ccacgataaa tgctatcgcc gcgcccactt cgacgccgga
     2461 gagttcgtcc agagttggga tgatgacgct gcccgcaaag gttactgcct gtacaaaatg
     2521 ggctgcaaag ggcctaccac ctataacgcc tgttcttcca cacgctggaa tgatggcgtt
     2581 tctttcccaa tccagtctgg tcacggctgc ctgggctgtg cggaaaatgg tttctgggat
     2641 cgcggttcgt tctacagccg cgtggtcgat attccgcaaa tgggtactca ttccaccgcc
     2701 gataccgtcg gtttaaccgc gcttggcgtg gtggcagcgg ctgttggtgt gcacgcagtc
     2761 gccagcgccg ttgaccagcg cagacgtcat aaccagcaac ctacagaaac cgaacatcag
     2821 ccaggcaatg aggataaaca ggcatgagca ctcagtacga aactcaggga tacaccatca
     2881 ataatgccgg acgccgcctg gtggtcgacc cgattacgcg catcgaaggc cacatgcgct
     2941 gcgaagtgaa tattaacgat cagaatgtga tcaccaatgc cgtctcctgc ggcaccatgt
     3001 ttcgcgggct ggagatcatc ctacaagggc gcgacccgcg cgatgcgtgg gcgttcgttg
     3061 aacgtatctg cggcgtctgt actggcgtac acgccctggc ttcggtttac gccatcgaag
     3121 atgctatcgg tattaaagtg ccggacaacg caaatatcat ccgcaacatt atgctggcaa
     3181 cgctctggtg ccacgatcat ctggtgcact tctatcagct tgccgggatg gactggatcg
     3241 atgtgttaga tgcgctgaaa gccgacccgc ggaaaacctc cgaactggcg caaagtctct
     3301 cctcttggcc gaaatcatcc cctggctatt tcttcgacgt acaaaaccgc ctgaaaaaat
     3361 ttgttgaagg cgggcagttg gggatcttcc gcaatggcta ctgggggcac ccgcagtaca
     3421 aactgccgcc agaagctaac ctgatgggct ttgcccacta tctcgaagct ctcgatttcc
     3481 agcgtgaaat tgtcaaaatc cacgcggtct ttggcggtaa aaacccgcat ccaaactgga
     3541 ttgtcggcgg gatgccttgc gccatcaaca ttgacgaaag cggcgcggtc ggggcagtca
     3601 atatggaacg cctgaacctg gtgcagtcaa ttatcacccg cacggcggac ttcattaaca
     3661 acgtgatgat ccccgacgcc ttagccatcg gtcagttcaa caaaccgtgg agcgaaatcg
     3721 gcactggtct ttctgataaa tgcgttctca gctacggcgc attcccggat attgccaacg
     3781 actttggcga gaaaagtctg ctgatgcctg gcggcgcggt gattaacggc gacttcaaca
     3841 atgtgctgcc agtggatttg gttgatccgc agcaggtgca ggagtttgtc gaccacgcct
     3901 ggtatcgata tcccaacgat caggtcgggc gtcatccgtt cgatggcatc accgacccgt
     3961 ggtacaaccc cggcgatgtc aaaggcagcg ataccaacat tcagcagctg aatgaacagg
     4021 aacgctactc gtggatcaaa gcgccacgct ggcgcggtaa cgcgatggaa gtggggccgc
     4081 tggcgcgcac gttaatcgct tatcacaaag gcgatgctgc gaccgttgag tcggtcgatc
     4141 gcatgatgtc ggcgttgaac ctgccgcttt ccggtatcca gtcaacgtta ggccgcattt
     4201 tgtgccgcgc gcacgaagcg cagtgggccg caggtaagtt gcagtatttc ttcgacaagc
     4261 tgatgaccaa cctgaaaaac ggcaatctcg ccactgcttc cacggaaaaa tgggaacctg
     4321 caacctggcc gacagagtgc cgtggtgtcg gttttaccga agcgccgcgc ggggcgttag
     4381 gccactgggc cgccattcgc gatggcaaga ttgatctcta ccagtgcgtg gtgccgacca
     4441 cctggaacgc cagcccgcgc gatcccaaag ggcagattgg cgcttatgaa gcggcgctga
     4501 tgaacaccaa aatggcgatc cccgagcaac cgctggagat cctgcgtact ctgcacagct
     4561 ttgacccgtg cctcgcctgt tcaacacacg tgctgggcga cgacggtagc gagctgatct
     4621 ctgtgcaggt gcgttaacag cgaaggagaa tcatcatgca acagaaaagc gacaacgttg
     4681 tcagccacta tgtctttgaa gcgccagtcc gcatctggca ctggttgacg gtgttatgca
     4741 tggcggtgtt gatggtcacc gggtacttta tcggcaagcc gctaccttcc gtcagcggcg
     4801 aggcgacgta tctgttctat atgggctaca tcaggttaat tcacttcagc gccgggatgg
     4861 tttttaccgt ggttttgctg atgcggatct actgggcttt tgttggcaat cgatactccc
     4921 gcgagctgtt tatcgtgccg gtatggcgta aaagctggtg gcagggcgtg tggtatgaaa
     4981 tccgctggta tctgtttctg gcaaaacgtc cgagtgccga tataggccat aatcccatcg
     5041 cccaggcggc gatgttcggc tatttcctga tgtcggtctt tatgatcatc actggttttg
     5101 cgctgtatag cgaacacagc cagtacgcta tttttgcgcc gttccgttat gtggtggaat
     5161 ttttctactg gatgggtggc aactcaatgg acattcacag ctggcatcgg ctggggatgt
     5221 ggctgattgg cgcgtttgtg atcggtcatg tctacatggc gctgcgtgaa gacatcatgt
     5281 ccgacgacac ggtgatctcc accatggtca acggctaccg tagccacaaa tttggcaaaa
     5341 taagtaacaa ggagcgttca tgagcgagca acgcgtggtg gtcatggggc tgggcaacct
     5401 gctgtgggcc gatgaaggct tcggcgtgcg ggtggcggaa cggctgtatg cccattacca
     5461 ctggcccgag tatgtggaga ttgtcgatgg cggtactcag ggactgaact tgctggggta
     5521 tgtcgaaagc gccagccatc tgttgattct cgatgccatt gactacgggc tggaacctgg
     5581 aacgctgcga acctatgccg gagaacgcat tccggcttat ctcagcgcga agaaaatgag
     5641 cctgcatcag aacagtttct ccgaagtgtt ggcgctggcg gatatccgcg gacatctgcc
     5701 agcacatatt gccctcgtcg gtctgcaacc cgcaatgctc gacgactacg gcggtagcct
     5761 gagcgaactg gcacgggagc aactgcccgc tgcggaacag gcggcgctgg cgcagctggc
     5821 tgcgtgggga attgtgccgc aaccggctaa tgaatcgcgc tgtctcaatt atgactgtct
     5881 gtcgatggaa aattacgaag gcgttcgctt gcgccagtac cggatgacac aggaggagca
     5941 gggatgagca acgacacgcc atttgatgcg ttgtggcaac gaatgctggc gcgcggctgg
     6001 acgccagtca gtgaatcccg tcttgacgac tggcttacgc aagcgccaga cggcgtgctg
     6061 ttattaagca gtgacccgaa acgcacgcca gaggtcagcg ataatccggt aatgattggc
     6121 gaattactgc gcgagtttcc cgactataca tggcaggtgg cgattgctga ccttgagcag
     6181 agcgaagcca tcggcgatcg ctttggcgtc tttcgctttc ctgccacttt agtgtttacc
     6241 ggcggaaact atcgcggcgt gctgaatggt attcacccgt gggcggaact gataaacctg
     6301 atgcgcgggc ttgtcgaacc gcagcaggag cgtgcctcat gagcgaaaca tttttccatc
     6361 tgctggggcc aggaacgcaa ccgaacgatg acagtttcag catgaatcca ctgccgatca
     6421 cctgtcaggt gaatgatgaa ccgagtatgg cggccctgga gcaatgtgct cacagcccgc
     6481 aggtgattgc gctgttaaac gagttacaac atcaactaag cgaacgccaa ccgccgttgg
     6541 gcgaggtgct ggcagtcgat ctgttaaatc tcaacgccga cgatcgtcac tttatcaata
     6601 cgcttctcgg ggaaggggaa gtgtcagtgc gcattcagca ggctgacgac agtgaaagtg
     6661 aaatacagga ggcgatcttc tgcggattat ggcgggtgcg cagacgtcgc ggcgaaaagt
     6721 tgctggagga caaactggag gctggctgcg cgccgctggc gttgtggcag gcggcaacgc
     6781 aaaatctctt gccgacagat tcgctgttac cgccgcccat tgatggcctg atgaatggcc
     6841 taccgttggc gcatgagtta ctggcacatg tacgtaaccc cgacgcgcag ccgcacagca
     6901 ttaatctgac gcaattaccc atcagcgagg ctgatcggct ttttctctca cgtctctgtg
     6961 ggccgggaaa tattcagatt cgtaccattg gctatggcga gagctatatc aacgccacgg
     7021 ggttacgcca tgtctggcat ttacgctgta cggacacctt aaaaggcccg ttactggaaa
     7081 gttatgaaat ctgcccaata ccggaagtgg tgctggcagc gccagaagat ttggtcgact
     7141 ctgcgcagcg gcttagcgag gtatgtcagt ggctggcgga agctgcaccg acgtaaaaag
     7201 acggtaagta tcgctttcag tcttatgaat atcgcaatcg gcgaatacct ctggtcgtag
     7261 agtttcagga taaagaggga gatctaccat tatcgggtta tttttctctc ttcgcctaca
     7321 ggagtgcgca tgtgggatgt cattgattta tcgcgctggc agtttgctct gacctgcgct
     7381 gtatcacttt ttatttgtac cccttaccct ggggctgatt tttttgctgg ctatcatgga
     7441 aaccatttac gtggtcaccg gcaaaacaat ctaccgcgat atgacgcgct tctggggtaa
     7501 gctcttcggt atcaattttg ctcttggcgt ggctaccggc ctgaccatgg agtttcagtt
     7561 tggtactaac tggtcattct attccaacta tgtgggcgat atttttggcg caccactggc
     7621 gatggaagca ttaatggcct tcttcctcga atccaccttt gtcgggctgt tcttcttcgg
     7681 ctggcaacgg ctgaataaat accagcacct gctggtgacg tggctggtgg cgttcggttc
     7741 aaatctctct gcgttgtgga tattgaatgc caacggttgg atgcaatacc cgaccggtgc
     7801 gcattttgat atcgacaccc tgcgtatgga gatgaccagc ttcagcgagc tggtctttaa
     7861 cccggtcagc caggtgaaat ttgtgcacac cgtaatggcg ggctacgtga ccggggccat
     7921 gtttattatg gcgatcagcg cctggtattt actgcgcgga cgggagcgcg atgtcgcatt
     7981 acgttcgttt gccatcggtt ccgtcttcgg tactctggcg attatcggta ccctgcaact
     8041 cggagacagt tctgcgtatg aagtcgcgca agtacaaccg gtaaaactgg cggcgatgga
     8101 aggggagtgg caaacggaac ctgcacctgc accgttccat gtggttgcct ggccggaaca
     8161 ggatcaagag cgtaacgcct ttgccatcaa aattcccgcg ctgctaggga tcctcgccac
     8221 tcactcatta gataaacccg tgccgggtct gaagaatttg atggctgaaa cctacccacg
     8281 cttgcaacgc ggacgtatgg cctggctgtt aatgcaggaa atatcgcaag gcaatcgtga
     8341 gccgcatgtg ttgcaggcat tccgggaact ggaaggtgac ctgggctacg gcatgttgct
     8401 ctcccgctat gcgccggata tgaatcatgt cacagccgca cagtaccagg cggcgatgcg
     8461 tggcgcgata cctcaggttg cgccggtatt ctggagtttc cgcatcatgg tgggctgtgg
     8521 ttccctgctg ctactggtga tgctgattgc gcttgtccag acgctgcgtg gcaaaatcga
     8581 ccagcatcgc tgggtgctga aaatggcgct ctggagtttg ccgttgccgt ggattgcgat
     8641 tgaagccggg tggtttatga ccgagtttgg tcgtcagccg tgggcgatac aggacatctt
     8701 accgacatac tccgcgcact ccgctttaac cacaggacaa ctggctttct cactgatcat
     8761 gatcgtaggg ctttacaccc tgttcttaat cgccgaagtc tacctgatgc agaaatatgc
     8821 ccgtctgggg ccgagcgcga tgcagagtga acaaccgacg cagcaacagg ggtaaaggag
     8881 aaaatcatgt ttgattatga aacattgcgc ttcatctggt ggctgctgat tggcgtgatc
     8941 ctggtggtct ttatgatctc cgacggattt gacatgggga tcggctgtct gctgccgctg
     9001 gtggcgcgta atgatgatga acgccggata gtgataaaca gcgttggtgc acactgggaa
     9061 ggcaaccagg tctggttgat cctcgctggt ggggcattat ttgccgcctg gcccagagtg
     9121 tatgcagcgg cgttttccgg cttttatgtg gcgatgatcc tggtgctgtg ctcactgttc
     9181 ttccgcccgc tggcctttga ttatcgcgga aaaatcgccg atgcacgctg gcgtaaaatg
     9241 tgggacgccg gtctggtcat cggcagtctg gtgccgccgg tagtcttcgg tatcgccttc
     9301 ggcaacttgt tgctcggcgt gccgtttgcc ttcacaccgc aattacgcgt ggagtatctc
     9361 ggcagcttct ggcaactgct gacgccattc cctttattgt gcggattgct cagccttggg
     9421 atggtgattt tgcaaggtgg cgtctggtta caactgaaaa ctgttggtgt gattcatctg
     9481 cgttcacagc tggccaccaa acgcgctgca ctgttggtga tgctgtgctt tttgctggcg
     9541 ggttactggc tgtgggtcgg tattgatggc tttgtactgc tcgcccagga tgctaacggt
     9601 ccttccaatc cgttaatgaa actggtggca gtgctaccta gtgcctggat gaataatttc
     9661 gtcgaatcgc ccgttttgtg gatcttcccg ctgctggggt tcttctgccc attgctgacg
     9721 gtgatggcga tttatcgtgg tcgcccgggt tggggatttt taatggcgtc attgatgcaa
     9781 tttggcgtga ttttcacggc aggcatcacg ctgttcccct ttgtcatgcc gtcaagcgtg
     9841 agtccaatct ccagcctgac gttgtgggac agcacttcca gtcagctgac gctgagcatt
     9901 atgttggtaa tcgtgctgat atttttgccc attgtgttgc tctacactct ctggagctac
     9961 tacaaaatgt ggggacgcat gacaacagaa actctccgcc gtaacgaaaa cgagttgtac
    10021 taaggagcag gaacaatgtg gtatttactt tggttcgtcg gcattttgtt gatgtgttcg
    10081 ctctccaccc ttgtgttggt atggctggac ccgcgtctga aaagttaacg aacgtaggcc
    10141 tgatgcggcg cattagcatc gcatcaggca atcaataatg tcagatatga aaagcggaaa
    10201 catatcgatg aaagcgatct taatcccatt tttatctctt ctgattccgt taaccccgca
    10261 atctgcattc gctcagagtg agccggagct gaagctggaa agtgtggtga ttgtcagtcg
    10321 tcatggtgtg cgtgctccaa ccaaggccac gcaactgatg caggatgtca ccccagacgc
    10381 atggccaacc tggccggtaa aactgggttg gctgacaccg cgcggtggtg agctaatcgc
    10441 ctatctcgga cattaccaac gccagcgtct ggtagccgac ggattgctgg cgaaaaaggg
    10501 ctgcccgcag tctggtcagg tcgcgattat tgctgatgtc gacgagcgta cccgtaaaac
    10561 aggcgaagcc ttcgccgccg ggctggcacc tgactgtgca ataaccgtac atacccaggc
    10621 agatacgtcc agtcccgatc cgttatttaa tcctctaaaa actggcgttt gccaactgga
    10681 taatgcgaac gtgactgacg cgatcctcag cagggcagga gggtcaattg ctgactttac
    10741 cgggcatcgg caaacggcgt ttcgcgaact ggaacgggtg cttaattttc cgcaatcaaa
    10801 cttgtgcctt aaacgtgaga aacaggacga aagctgttca ttaacgcagg cattaccatc
    10861 ggaactcaag gtgagcgccg acaatgtctc attaaccggt gcggtaagcc tcgcatcaat
    10921 gctgacggag atatttctcc tgcaacaagc acagggaatg ccggagccgg ggtggggaag
    10981 gatcaccgat tcacaccagt ggaacacctt gctaagtttg cataacgcgc aattttattt
    11041 gctacaacgc acgccagagg ttgcccgcag ccgcgccacc ccgttattag atttgatcaa
    11101 gacagcgttg acgccccatc caccgcaaaa acaggcgtat ggtgtgacat tacccacttc
    11161 agtgctgttt atcgccggac acgatactaa tctggcaaat ctcggcggcg cactggagct
    11221 caactggacg cttcccggtc agccggataa cacgccgcca ggtggtgaac tggtgtttga
    11281 acgctggcgt cggctaagcg ataacagcca gtggattcag gtttcgctgg tcttccagac
    11341 tttacagcag atgcgtgata aaacgccgct gtcattaaat acgccgcccg gagaggtgaa
    11401 actgaccctg gcaggatgtg aagagcgaaa tgcgcagggc atgtgttcgt tggcaggttt
    11461 tacgcaaatc gtgaatgaag cacgcatacc ggcgtgcagt ttgtaatgca taaaaaagag
    11521 cattcagtta cctgaatgct ctgaggctga tgacaaacga agaactgtct aatgcgtaga
    11581 ccggaaaagg cgttcacgcc gcatccggcc actttcagtt ttactctttc tcggagtaac
    11641 tataaccgta atagttatag ccgtaactgt aagcggtgct ggcgcgttta atcacaccat
    11701 tgaggatagc gcctttaata ttgacgcctg cctgttccag acgctgcatt gacaaactca
    11761 cctctttggc ggtgttcaag ccaaaatgcg caaccagcag gctggtgcca acagaacgcc
    11821 ccacgaccgc ggcatcactc accgccagca tcggcggcgt atcgacaatc accagatcgt
    11881 aatggtcgtt cgcccattcc agtaattgac gcatccgatc gcgcatcagc agttcagacg
    11941 ggttaggtgg cacctgaccg cgagtaatca catcaaagcc tcctttgcca aaatgctgga
    12001 tcactttgtt gagctcatct ttacctgcca gatattccga caagccatgt tcattactca
    12061 cggtaaacag gttatgcgaa taaccacggc gtaagtcggc atcaataaat aacacttttt
    12121 gatcggactg ggcgatcacc gctgccagag ttgaactgac aaacgtttta ccactgtctg
    12181 gcgtcgcacc ggtgatcatc agaatgttat tctccgtctc catcatagcg aaatgcagac
    12241 tggttcgtag cgcacgtacg gcttccacag cagaatccgc cgggttatcc accgccagga
    12301 aggggatatt tttagtacga tggcgctgct gattagaaaa taaatttttc ttacgcagac
    12361 gggtgcgttt atccagccac tcggacattg ggatagtggc ataaacgctg atgccgtgct
    12421 cttccagttg ttccggggct tctacaccac gacgcaacat cgcacgcgcc agcacggcac
    12481 ccacagaaat aaacaggcca agaataaaac caagcaccac attcaacgct tttttcggtt
    12541 tcactggctg cggctgagtg actgccgggt cgataatccg cacgttacca atggcactgg
    12601 atttcgaaat actcaactcc tgctggcggt taagtaattg cagatatacc gcacggcccg
    12661 cttctacgtc acgacttaaa cgcaacactt cctgttgggt ggaaggcatt gccgataccc
    12721 gcttattcag gcgtttgcgt tcttgctcca gcgtctggcg tttttccagc agcgcacgat
    12781 aagttgggtg atctttctta tacagctggg agatctctgc ctcgcggaag gtcagctcat
    12841 tgagttgatt atcaacgttc acaatctgct caagaacggc tttggcttcc aggttaaggt
    12901 caaccgaatc gcgctgctgg cgataaacgt tgagtttttc ttccgcctgg tccagctcgc
    12961 tgcgcacttc aggtaactgg cgctgtaaga attcaaggct ttgtgaatcc tgcgccgcct
    13021 ggcgagcgat attctgttgc aaatagttgt tagcgatgct gttcagaata cgagtaatca
    13081 actggggatc atcaccagtc atggtaagtt ccagcatccc gctttcttta ctgcgttcgc
    13141 taacggtaaa ggtttcctgc aatgcgttaa tcgcttccag ttcggtacgc tggctcagga
    13201 caaactgtgt tcctggtttg gccttaatgt ccgcgatagt cagcgcaacg ccatcttttt
    13261 ccagacgctg gccgaccata ccattgacgg tgaactcttc accttccagt gtatagtggc
    13321 cgttttcccc aaccgtgagt gtcagttgct gagcctgacc attcagttgt ggaatatgca
    13381 tccagctgat cgccagctca cctggttttt ctttggttaa tctcgcccag ccgcgaccca
    13441 caatcggaaa atacttctgc tcaactatgt cgcgcagatt cagttcagca atggttttac
    13501 cgagaatcat gcgcgattgc agcagttgga tctccggtgc agactcgggc gatgagttag
    13561 ggatcatatc gctcaggccg ctgagaatgg cgttgccctg tttttgctca acctggacca
    13621 gagtatctgc ctgataaatt ggtgtgctta acagcgagta agcgacagcg atcagcgtga
    13681 ataacgcggt cacgctgata ataaacttac ggtgatccca taactcgccg accagacgaa
    13741 gcagatcgat ctcattttcc tgagtgctgc ctggtggcgt attcatattt ttagttgtca
    13801 tcgtatttta taaactcaaa ttaccggctg aggcgcttcg cccattcctg actggcgcgc
    13861 tccaacatac cgtagacatg ttcaaatgcg tcctgacttt tacgataggg atccgggatc
    13921 tctttctgtt ccagccattg cccaaacagc attgtcttcc cgcggacctc gggagcaatt
    13981 gcggtaacct gagcaatatg ttccgactcc attgccagaa tcagatcgta atttcgtgcc
    14041 atctcagcag tgagcttacg tccggcatgt ccttcaagag atacgccatg attagcggca
    14101 acatctgccg ctgtcgcatc tgcagggtgt tttaccagac catgaacacc tgccgatttg
    14161 acttttacgc ccggcagacg tttacgcagc aagcgctcgc caatgggcga acggcaaatg
    14221 ttgccggtac aaaccaccag gattgagtta aattttagtt gggccatgtt tgaatccacc
    14281 gtgaagtctc tgtcaggtca tgcaccccgg aaatcgtcgg taccagcaga gaaatcacac
    14341 ggttccaacg agccagcggc gcggtagtga cataaacaat gtcgtaaggt tcaagctgga
    14401 attccgttcc gagtatcatt gccgatgcgt ccttcgcatt cagctgataa atattggcga
    14461 ttttgccatt ctgcttgttt tgcgttgcac gaataacaaa aatccccgtc gcatcagcga
    14521 catcctgatt cataccctca gcgttaccca gcgcttccgc cagggtcata ccgctgcggt
    14581 ccattttcag cgtgctctgt ttacccactt cacccatcac gaagactttc agatcatcgt
    14641 tgcgcggaat aaataaaata tcgcccgggt gcagcagttt gttctggcgc aagtcgccac
    14701 gctgcatcag cgcatagagg ttcactttgg tctttacgcc attctgggtg agcaccacgt
    14761 tgcgccagtc tgcatctgcg gtcagaccgc ccgcggcgtt aatcgcatcc atgatggtca
    14821 gaggaatatt ggtaatcggc tgctgacccg atttgctgac ttcgccggtg acgtacgctt
    14881 tttgcgagcg gaacgccgcg acgctgacat cgacttgcgg gctttcaatg actgaatcca
    14941 gacgcgctgt aatctcgttg cgtacctggg taagtgtttt cccggccact ttcagacgac
    15001 caatataggg gtagaaaatc gcaccatcgg cgttaaccca gttgccggta tcactggcgc
    15061 tacggtattg ccctgcgggg gtggttagtt ccgggtgatc ccagaccgta accatcaaca
    15121 catccccaat gccgatacga tattcatagt ttgcgatttg ctgctccagc tccgggttag
    15181 cctgcgacat aatcggtttg gcgcgcaact gatcaatcaa tcctggcgtg acgggataga
    15241 tattgaccat tttgtccagg tcatactgat tgtcaggtag ttcaatgaca tccttgttac
    15301 tggtcgataa attctgacca ggaacaaggg tacatgctgt caaagacaga acagccaatg
    15361 tcagtaccga aaacttaaaa atgttctttt tcattggttt atatttatca gtcaatgtaa
    15421 tgttatgaat tacgtcaggt tattgtccaa ccggtacttc tcgctctgcc gtcattggat
    15481 agagctggta tttgcgtgac agcatttgtc caccatcacg cgtcaatggt gtccacgaaa
    15541 ccaccgcgcg gttgcggtta ggtccaatgg tcatcaagtc gaatggaatc gagatataaa
    15601 aacctttact aaagccgcct tcgccgtagt catctttaga tacgttactg attgccgccc
    15661 atacccctac cgccacgccg ctgtcaaagc gtttggcgac gtcgatcgtt gcgcctttat
    15721 cttttgccag atattgccca acgctaagtt tcatcagtac gccattgagc gtcggcgggt
    15781 tccagtaagc cgtcacgaaa cctgttggcg tggaataatc ggtgaaacgc atcatgttgt
    15841 cccagtcacg ttgcttcacg tagttaacgt ccacacccag cgcccagctg gcatctagcg
    15901 ggcgataaag cagctcggaa ccgacaccgg cgtacatcgt ttccagataa ccgccataca
    15961 cctggccata gaaaccattg cctaagtcgg caaagtagtt cgcctgcaag ttgttgagat
    16021 aaacgtcatt gcgaacgtaa tcacggatat gcgtgcgcac gcggggcagg gtagagtccg
    16081 cgggcaacag cgaagactta aacttgtcgt agttgttgta aatattggtg aaaataccgc
    16141 catcaagcag caggtggtcg gtaaaccagt agcgggcact ggacatcagc cccagctgga
    16201 acatatagaa atcttccggg ccgcccagcg actgtgaaag tgttgggttg aaagagtagc
    16261 taaagcgatc ttcacgaata cggtagcccc gaccaaaggc agaaagatct tctgcttcaa
    16321 cacgttgttg ttgaagttgc tctgattgac caggcgctgt accagccagc tgtttgcgca
    16381 ggctggctac gtcggtttcg gtagtcacca tcgccatatg ctcgcgcttt tgcgtcacgc
    16441 taatcttctc aacgccttgc ggcaggttat tcaccagaat ccggttggca cgatcgaccg
    16501 cttcgcgaga atcacggtat ttgtattgtt gaccagacat atacagcgtc ttatcgcgca
    16561 gctgaatttc cggtgcttca aaacctgcgt tgtacttcag cgcggtaagt tggttcgcta
    16621 ctgtggtgta ctgcaatcct tcagattcag gcgcaggttg atatgccggt tttggcgtat
    16681 cgcgcagggc agggcgcaga tcgttgaaat tggtccgtaa cgtgaagcca aacatcaacg
    16741 tgttaccgcg ttcataactc aggttgagat ctgcccagct ggcagcgcga taaactgcgc
    16801 cgacgttgaa atggcttgcc tgaggcagtt tgccagcgaa atcattctgg taattgttgc
    16861 catcgtattc gagtttcaga cgcaggggat tccacggcgt ttgatactca atgccgccaa
    16921 agatggaagc cgggccacga aagatatcgc taaagctgat atcgcccgca tcgtgagact
    16981 ctgcgcgatg acaatattta tcgctcaccc ggcaaaacgg gttggtaata ttgcccgcat
    17041 taccggcgta gccccatgcc atcccgaggg tgaaatcaaa tggccccgct tgcttgctgg
    17101 ccaccagata ctcaccgtca aacagacccg taccagcaat atcacgttta ccaaacgcca
    17161 cttgcggtag ccagtaacct tcttcccaca ggcgaagttt aaaatcgaat gatttgtctt
    17221 tgtatgactg atcgccgctg aaatcttccc actggctata tttgcgtgtg cgcacatccg
    17281 tataacgaat ggtgccttcc agccatggga acagcgccac ggaggtggag tagaaccggt
    17341 attgatcgtt atcccggtag ttgacgctga attcaccttc cggtgcgatg cgcgcatttg
    17401 gcatctgcaa caatcctgtg ccgccaaagt ctgattgcga cgaacccagc ggatccgggt
    17461 aggttaatac ttcagcatga caggcactgg agacggcaat ggccaggcag cttaaaagat
    17521 aagaattctt cttcatcagt caggaacacg ttgcgttaaa acagacacaa tctggtcatt
    17581 taaatcagca tatttttcgg gcagtacatg cgcagaaaag cccaaccaaa gctggctgcc
    17641 aggcggtggt tccacatgtc ttttattcca cagggcaaca ggggcgacga cagtctcacc
    17701 ctcaggcgta atcaccataa cgttattctt gtccgcgcct gcaaggcggg ggtgatcttg
    17761 cagataatcg gtaacgctac ggcctgcctg ccagggcaat tgcccggccc cagaaaccgc
    17821 acccagcaga gtaatagtca ccggacgctg tacggtatag agcgtatagt ccccgaccaa
    17881 cgggggattg ctgttctcat ccactcgtac aaaatcaggg tccagtttga ccggtaagcg
    17941 accagtgata ttgagattga gtaactgctg gcgcacggat ttaatcgttg ccgccacatc
    18001 gtcgtcagct tctgcttccc aggaagcaag ctgtgccatg acatgttggt agtctttaag
    18061 cgctttagct ttcgccgcag agtcggtcag caaggcacca ggccaccaga ggcgatcgcg
    18121 cagttgcggt tgtgtcacca actggacaac attttccatg gggccaacgg aaagtgtctg
    18181 ttgttcgcca ggcagataaa tagtcaccgt tccttgcgca aaggcatggg gtgtcattac
    18241 gtaaagtacg ctggcaatga aatacgactg taatttattc atggtgcggg tttaagaaac
    18301 gtcatttcta cgggaataac ccctgcgcct aacatttgcc gactttgacg aacctggcca
    18361 gtagccgaat cgacccagaa tgtgttttgc cactgttttt ctggccgcgt ggaagtcacc
    18421 tcttcctgcc agacattaca cagcacattg cgacccgcaa tattcagcgt ctctaatcca
    18481 gcaaaggaaa aagtagaact cagggtcgca gagcgaaaat ggttgtcttc actccagaga
    18541 atatcgcgcg tccagcggct gccatctttt attgccagcg cgttacgcag agggtcctgc
    18601 ccggaatttg tcacttccag cagattatta ttaagtttga cggtttttaa aagacgtcca
    18661 ttgtgggtaa ccagcatggc gttatcctgg gataaccatt tgctttgttc ttgttctata
    18721 tatcccagta ccacaaagat tcgttgccct tcattaaggc gtaaatacat cgtggaataa
    18781 ggcaacgcct ggatctgctg atccgctacg gtgatatcct gattatcgaa aaggctggcg
    18841 cgaaatgtat cgaccatact ttgctggctg tgcgtacatc ccgcaagaaa tagtgcgaaa
    18901 atcgataaaa taagagggcg cacctttaat ccttgaaagg ttaattaaaa aaatgcaaat
    18961 ctctaaagcc cggtacgaat atcgcaaccg ggtatcattt aactttaatt tcgacgcgtt
    19021 aaagcagtat tagcgtgtag tggacgtggt ggttgaggtg ttatgagaac cgccatcgcc
    19081 actgtcattt gctgcaacaa ctgccaacgt cgccacacca acagccgttg ccacaccgac
    19141 aactgtcgcc gtgccgacag cgccagcagc cacgccacca gtggtagtag cagtcgtggt
    19201 tgcgccggta gtggtcgttg tggtgccagt agtacccgtc gttgcctcgg tcccgtttgt
    19261 tgcttcaggg gcagcaaacg cagctggcgt cgtcaacatg aaggccataa ggattgcaga
    19321 aagcttgtgt ttcataactt ttcctttatt catcgcatgg acaatacgta cgcttgagat
    19381 caggattgtc tactttgagt ataacatcct tgtgaaatct ccctttcctc ataccgcttg
    19441 cactatatac tgcgggtagg aaaggcgcgc agagggaaat ataagattgt taactatccc
    19501 cctctggaaa tacgacttgt gagcataaga taaatcttag caaaatacaa attaaagata
    19561 aggaaattgt tacgaaagct attaattatt gttggtaata tagtttcaag tggtactata
    19621 gctggctgtt aaatggcatt tataactatt aggtgcagag atattcgctt aaatggatta
    19681 gtttctttgt ggaaaagtaa ctggtgttat aatgtatgat gttgactatt gcactattcc
    19741 aataaaacca gtcagcttta aacaagcagc gtcatattaa gagagataaa catttgccgc
    19801 tgttggtcct cgcaggccat ttacgcggca aaattccaca cgtaatcctg gtataagcac
    19861 ttctgcgtcg cggggagtga atgcggaaat atggacctga acttctttac gaccgtcgga
    19921 ggggataatg aatcctttgc cgcttttgcg atcaaaggtt ttgacaattc ctgtcatttt
    19981 acgggacaaa caaattcctt actgaaaata ctgcgctgca ctatacgggg ttaataaaat
    20041 aaagccagcg atatttaaga ccgccggacg gctaaaataa aatttgctta atctcaatta
    20101 tcatgcgtta atagctgcgt cggtttgaaa gacagacagc atacaaagta gtttactaaa
    20161 gcagttctca ttatcaggca ttatcccctt cttttgagtc tctctcctga acactaagta
    20221 gtttctgtat taaagccctg tttgccgaaa ggcccaaaat gaaggaagta aaatatgtct
    20281 aataaaatga ctggtttagt aaaatggttt aacgcagata aaggttttgg ctttatcact
    20341 cctgatgatg gcagcaaaga cgttttcgtc catttcactg ccatccagag caatgaattc
    20401 cgcacgctga acgaaaatca gaaagttgaa ttttctattg agcaggggca acgtggcccc
    20461 gcggcagcga acgttgttac gctctaaggt tgccgttatt actcaacatc tccatttccg
    20521 ctgtccatgt tgtcatggtt cacagtaccg tacatcggca ttcgatgtga cggagcgaaa
    20581 ccctttgggc gctaagtgta ttttttgtaa atcgacgatg atcacctttg ataacgtcgc
    20641 gctgcaaata cgcacggacc atgcgccgct ggatttcaca aaataatatc aggctccctc
    20701 gtggagcctt ttttatatct gccttatttt tcttcaacgc tgtatgtata gtaagcgata
    20761 acctgttgat tattgaatct ttcggggaga tggcttataa catttcttac ctgaccaggg
    20821 taccgggaac caacacctta ctggcgtgtt gctgtctttc aagaccagaa gaggttaaca
    20881 gtgaatattg aagagttaaa aaaacaagcc gaaacggaaa tcgccgactt tatcgcgcaa
    20941 aaaatcgccg agctgaacaa gaatacaggg aaagaagtct ctgaaattcg cttcaccgca
    21001 cgagaaaaaa tgaccgggct tgaaagttat gatgtcaaaa tcaaaataat gtgattttgt
    21061 gaacatcacc cgtgcgaggt gatgttccgc ttgttgctaa tttagtgacc aatcattggc
    21121 gcttgtggaa ttaagcgtcg gtacaattca tccggtaccg ggctttgcca tactcccgca
    21181 tacattgcgt aaccaatcac cgcaaacata atccccagaa ccagtagcgt cattaaccag
    21241 ccagacaacg caaaggcttt tttatttgcc gcaggttttt gcagtgaaaa ggtcaatgtt
    21301 gaggctaccg gacatgactc tacgcaagtc atacagccgg tacattccac tgttcgtacc
    21361 tgaattaatt tatcgaccgg gatccgtgat gggcaatttt ttgcgcattt gccacagtcg
    21421 atacaacttt cggcattgcg acgaatctta aacggcgaca atagcgaaac cacgcccatc
    21481 agcgcgccat atgggcaaag ataacgacac caggcatggc gaataaacag gctggcaatc
    21541 agcaaaacgg tcacgctgat taatgtcgcg gtccccatat gacgaaagaa atcgagcatt
    21601 ttaacgtcca tcaccacgct gtagggcgac aacataaaat agtgaatcgc ctgagcgggc
    21661 atcaataatg cgatatagag aaaaaaactc aacagcaaat acttcacgcc gcgcagagga
    21721 atatccagcc agcggggaag gacacattgc cgaccaaaca gtttgttacc gagatcgccg
    21781 attaattcag aaagcgtacc aaccgggcat aaccatgagc aaaaggcctt tttgagtaat
    21841 agactgatga cgataaaagc gaccaataac agcatcgcgg cggcgtggac ggacggtaac
    21901 tgacctgttg taaggctata tttcagattc atcagaccgg caatcggtag ccagccttcg
    21961 atacctcccg gtctggcgac aaatgtcgtg ctacttgccg tttcgtaata gcgcacccaa
    22021 taccagaacg tgatggcaat ataaatattc attgccaaca gtaataattg cgtcgcttta
    22081 cgccaggtcg tggcattgcg ccagtcattc cacggtaatt tgccgcccgt cgtgcctggc
    22141 cgccgctgcc agcgggttct ttttttctct gccatgattt tgccagtccg ttaagttgca
    22201 taccaaatgc cactattcta gttgttctta actggctgat attgattcaa atcgcgttca
    22261 ggtctttctt attcaaccat gcttccagat cggcaacact gcgtgtaatt tcttcgtgtg
    22321 gaaggggggc agataatggc tgctgctcca gttgtgcgca tagctggctg gcgatacgca
    22381 ttcccaaact cgagcaactg ctttttagct gatgcgcggc acgctttatt ttctcgctat
    22441 cctgactggc gcgggcaatg tcgatttcat cgagaagcgg cagggcatgt tgtgtaaata
    22501 ataccagcca ttcgtggatc ttctccgtcc ccattaactg agcatcttca ttgagttgcg
    22561 atacatccag cgattgatcg ttattgactt gcagttggag atagtgcgcc agtaactgac
    22621 cgagcacttc acgcggcacc ggtttaggga taatcccgcg gaataatgaa ctggtacgct
    22681 ggcgcagcgt ttcgtcaatg acatgggcgc taaagccaat caaaaccagc gacggatatt
    22741 gctgtgccag ttgtcgggca agcgtaatgc cgtcgacatc cggcagatca aaatccacca
    22801 gtgcggcagc aaacggttcg ctattttgca gtgtctctaa agcctgcgcg gcattgccaa
    22861 cagcaacaac ctgcgcacca ctggtgttca gcatctcaac ggtaattcgc tgggttagcg
    22921 ggttatcttc aattaacagc aaacgtaaac cgtcaagacg caccgcctga ttgactgttt
    22981 ttggcacggg tgccgtggca acacgtaacg gcaagcgtaa acaaaaacag cttccaacct
    23041 ccggcgtgct ggtggcgctc agttcgccgc ccatcgcctg ggccagacgg ctactgatag
    23101 tcagccccag cccggtgccg ccgcgtttgc cgcttacctg cacaaatggc tggaagattt
    23161 ctgccagttt cgcgggatca ataccgcagc cgctgtcttc cacttcgacc agccattgct
    23221 cgccatcagt gcgactacgc aggataatgt acccttcgtc agtaaaacgc agggcgttgc
    23281 tcaacaggtt ggttataacc tgacgaatac gtcgtggatc gcccattaac gcgcacggca
    23341 tatcatcggc aattgccgtt gccaggcgaa tcgggcgacc tttcacccgt ccgctcatta
    23401 attgcagggt actttccagc agcgggcgcg gttcaaaggg ctcatcgctg accgaaacat
    23461 tcttgccacc tgcttcgata gcggaataat cgagaatatc gttgaggatg gtcagcaacg
    23521 attcaccaga atcagtaatt gcccgcaaat catcacgctg ggcgttaagt gcggggttat
    23581 ctgccagcag ttgagcagtg ccgagaatac cgtacagcgg tgtgcggatc tcatggctca
    23641 tcgccgccag aaacgccgat tttgcctggc tggctttttc tgcttccgcc cgtgcctgtc
    23701 ggtgttctat caccagttcc tgcaattcag ctgtacgcgc tttgacctgc gccgccagct
    23761 gttcgcggtg gcgattcagt gcatgaacat tgctgcgaaa cgcatccatc agccgcccga
    23821 tggtatccag ctcccgtacg ccagcggttt ccgggaaagg ggagtcaata tcaccgtcca
    23881 gcagccgttg cagcgcctgc gtttgttcgg caagtggacg cgtgactgag cgataaacca
    23941 cgcgccagag gatcagaatc agtgcgcaaa gagaaactat tcccagcaat aacaggctgt
    24001 attgcccgcg tgcactcgct ttttccagat gcgccagtcc gtgctgatta cgcagctcaa
    24061 tggtatcgac cagctgactg acttcgctac taaactgcgc gaactgggcg atgttatttt
    24121 gggcgagagt ttgcaggtga gtgctgattt cactgtcctg ctgatacagc gccagtagat
    24181 cgctatattg gctaacggta gttaacgttg ttgcgacctg cgcacgaact cccggatctt
    24241 caatgcgtat ttgccgacgt tgcagaattt tcaccgcatt attgagctgc ttttccagcg
    24301 ttggtgcatt tttctggatc tgctccagcc ccagattcat caccatttgc tgcacccgca
    24361 gagcgctaag gcgcagttca ttcatctggt taacatactc aagatcgata tcaatcagcc
    24421 gatcgagtgc actttcagca gcctgacgct gatcttgttc gatcaaatcg taaatcccgg
    24481 cctgggtcgc tccagcggaa gttgtcgcat tattcgcctg accttgcgcc aggcgtgcga
    24541 tctcatcggc ggcagcgact atctgctgac tgagttgccg ttgttgctgg cgtagttgca
    24601 gacgccgccc caccagttcc ccttgctgac gtaatgaacg ggagatctcc tgctcctgtt
    24661 gttcaatagc ggtggtatca aaaccttgtt cccgtaacgc ttgcagcaac gcattaatct
    24721 tcaggctttg tgcggtgagc attcgcccct gcgcctgcca catcttttcg ttatcggcac
    24781 tggtcaggtt ctgcgcggcg aaaagttccc aggcgctggc ttcgctcaac tggcgcgcca
    24841 tattcatggt aggaatcaat gcctgagtgt tgtctttttc cacctggctg ataaagcgca
    24901 ggttgtacca tcccaccagg gtactggtca gggttaacag cgccatcagg gcaaagccca
    24961 tccagagtct tcgggtcagg gttaaattca cggtcggtgc acttaaggtg aaaaaggttg
    25021 agtcgcaaag cggaatgcat ctagcataaa gccttattat tgatgaggct atcatgcgcg
    25081 tactgctatt tttacttctt tcccttttca tgttatcggc attttcggct gataacctgt
    25141 tgcgctggca tgatgcgcag cattacacgg tgcaagcctc tacgccgctt aaagccaaac
    25201 gcgcatggaa actgtgcgcg ctttatccca gcctgaaaga ttcatactgg ttatcgttga
    25261 actatggtat gcaggaggca gctcgccgct atggtgtgga tttaaaagtg ctggaggcag
    25321 gcggctacag ccagttggct acccagcaag cacaaatcga ccagtgtaaa cagtggggcg
    25381 cagaggccat tttgctcggt agtagcacta cgtcatttcc cgacctgcaa aagcaggtag
    25441 caaatctgcc ggtgatcgaa ctggtaaatg ctcttgatga tcccctggtg aaaagccgcg
    25501 ttggtgtacc ctggtttcag atgggctatc aacctgggcg atatttggtg caatgggcgc
    25561 acggtaaacc actgaacgtg ctgttgatgc ccggacccga taatgccggg ggcagtaaag
    25621 agatggtcga gggttttcgc gcagccattg ccggaagccc ggtgcgtatt gttgatattg
    25681 cgcttggtga taacgatatt gaaatccagc gtaacctgtt gcaggagatg ctggaacgcc
    25741 acccagaaat cgacgtcgtt gccggaacgg ccattgcggc agaggcggca atgggtgaag
    25801 ggcgtaacct gaaaacgccg cttaccgtgg tgtcgtttta tctttcacat caggtgtatc
    25861 gcgggctgaa gcggggaaga gtgattatgg ctgccagcga tcaaatggtc tggcaggggg
    25921 aactggcggt tgagcaggcc atcaggcaat tacaggggca atcggtgtct gataatgtca
    25981 gcccaccgat tttagttctg acgccgaaaa atgccgaccg tgaacatatt cgccgctcgc
    26041 tgtcaccagg gggatttcgt ccggtctatt tttatcagca cacatcagcg gctaagaaat
    26101 aaccttcacc atgttgcgtc accagtaaat ccgcgctgag tttatgacgt aaacgacgaa
    26161 ttaacacatc gacggtgcgc aggtcagggt tttccacccg acgcgcagaa agcatacgta
    26221 gcagacgttc acggctgaga atttcgcccg gattcgtcac aaatgccacc aacatttcat
    26281 actctgcgcg ggtcagttta atcggctcgc catcccgctc cagcgtatgg cgcgacacat
    26341 tcaggcaata accggcaaag cgatagcagt tgtcctgagt gtgcggttga gcttgtcgcg
    26401 cgaggtcgat tcgccagagc agatttttca cccgtactac cagttcgcgc agttccagcg
    26461 gtttggtgac gtaatcgtct gcgcccattt ccagcccaac aatacggtca atccgatcgc
    26521 tgcgtccggt aaccagaata atccccaccg ttgagcgttc tcgcagggcg cgggttaaca
    26581 tcaggccatt ttcatcgggt aagttgatat ccagcagaat taaatctacc ggctgattct
    26641 gcataatttc ccgcagcccg gcaccgctcg ctgtaacgga aacggtatac ccctcctgag
    26701 tgaagtaaga ttgtaatcgc gcctgggtaa ccggctcatc ttcaacaata acaatgtgat
    26761 gtggcatcag agggttttac tcattctgtt catatctgtt catattctgc cgtaagccgt
    26821 tcatcctgac cagtgccgct gttcatattt gctcattaag atcgcttcac taaaccataa
    26881 ttttacaggg gctattatgc ggaaactctg gaacgcgcta cgccgaccca gtgctcgttg
    26941 gtcggtactg gcgctggtcg caattgggat tgtgattggc attgcgctga ttgtattgcc
    27001 acacgttggg atcaaagtca ccagcacaac cgaattttgt gtcagttgcc acagtatgca
    27061 accggtgtat gaagaatata aacagtcggt gcatttccag aacgcctccg gcgtgcgagc
    27121 tgaatgccat gactgtcata tcccgccgga tattccaggc atggtgaagc gcaaactgga
    27181 agcgagcaat gatatctacc agacctttat tgctcactcc attgatacac ctgaaaaatt
    27241 cgaagccaaa cgcgcggaac ttgccgagcg tgaatgggcg cgaatgaaag aaaacaactc
    27301 ggcaacctgc cgctcctgcc ataactacga tgcgatggat catgcgaagc agcatcctga
    27361 agcggcacgt cagatgaagg tggcagcgaa agataatcaa tcctgcatcg actgtcataa
    27421 aggtattgcc caccagttac cggatatgag tagcggcttc cgtaagcagt tcgatgagct
    27481 gcgcgccagt gctaatgaca gtggtgacac gctgtactct attgatatta agccaattta
    27541 tgcggcgaaa ggcgataaag aagcctctgg ttctctgctg cctgcttcgg aagtgaaagt
    27601 ccttaaacgt gacggcgact ggctacaaat tgaaattacc ggctggacgg aaagcgccgg
    27661 acgtcagcgt gtactcaccc aattcccagg taaacgcatc tttgttgcct cgattcgtgg
    27721 tgatgtgcag cagcaggtaa aaacgctgga gaaaaccacc gttgccgaca ccaataccga
    27781 gtggagcaag ttgcaggcca ctgcgtggat gaagaaaggc gacatggtga acgatatcaa
    27841 accgatctgg gcttatgcgg attcgttgta caacggcacc tgtaaccagt gccacggtgc
    27901 accggaaatc gcccactttg acgctaacgg ttggatcggc acgctcaacg gcatgattgg
    27961 ctttaccagt ctcgataaac gtgaagaacg caccttgttg aaatatctgc aaatgaatgc
    28021 gtctgacacc gcaggtaagg ctcacggcga taagaaggaa gaaaaataat gaacaataac
    28081 gatctctttc aggcatcacg tcggcgtttt ctggcacaac tcggcggctt aaccgtcgcc
    28141 gggatgctgg ggccgtcatt gttaacgccg cgccgtgcga ctgcggcgca agcggcgact
    28201 gacgctgtca tctcgaaaga gggcattctt accgggtcgc actggggggc tatccgcgcg
    28261 acggtgaagg atggtcgctt tgtggcggca aaaccgttcg aactggataa atatccgtcg
    28321 aaaatgattg ccggattgcc ggatcacgta cacaacgcgg cgcgtattcg ttatccgatg
    28381 gtacgcgtgg actggctgcg taagcgccat ctcagcgata cctcccagcg cggtgataac
    28441 cgttttgtgc gcgtgagctg ggatgaagcc ctcgacatgt tctatgaaga actggaacgc
    28501 gtgcagaaaa ctcacgggcc gagtgccttg ctgaccgcca gtggttggca atcgacgggg
    28561 atgttccata acgcttcggg gatgctggcg aaagctattg ccttgcatgg taatagcgtt
    28621 ggtacgggcg gagattactc taccggtgct gcgcaggtga tcctgccgcg cgtggttggc
    28681 tcaatggaag tgtatgaaca gcaaacctcc tggccgctgg tattgcagaa cagcaaaacc
    28741 attgtgctgt ggggctctga tttgctgaaa aaccagcaag cgaactggtg gtgcccggat
    28801 cacgatgttt atgaatatta cgcgcagcta aaagcgaaag tcgccgccgg tgaaattgag
    28861 gtcatcagca tcgatccggt tgtcacatcc acccatgagt atctggggcg cgagcatgtg
    28921 aagcacattg cggttaaccc gcaaactgat gtgccgctgc aactggcgct ggcgtatacg
    28981 ctgtacagtg aaaacctgta cgacaaaaac ttcctcgcta actactgtgt gggttttgag
    29041 cagttcctgc cgtacctgct gggtgagaaa gacggtcagc cgaaagatgc cgcatgggct
    29101 gaaaaactga ccggcattga tgccgaaacc attcgtgggc tggcgcggca gatggcggcg
    29161 aacagaacgc aaattattgc tggctggtgc gtgcagcgta tgcagcacgg tgaacagtgg
    29221 gcgtggatga ttgtggttct ggcggcgatg ctggggcaaa ttggcctgcc aggtggtggc
    29281 tttggttttg gctggcacta taacggcgca ggcacgccgg ggcgtaaagg cgttattctg
    29341 agtggtttct ccggctctac gtcgattccg cctgttcacg acaacagtga ttacaaaggc
    29401 tacagcagca ccattccgat tgcccgtttt atcgatgcga tcctcgaacc ggggaaagtg
    29461 atcaactgga acggtaaatc ggtaaaactg ccgccgctga aaatgtgtat ttttgccgga
    29521 actaacccat tccatcgcca tcagcagatc aaccgcatta ttgaaggctg gcgcaagctg
    29581 gaaacggtta tcgccataga taaccagtgg acctcaacct gccgctttgc cgatatcgtg
    29641 ctgcctgcga ccacgcagtt tgagcgtaac gatctcgacc agtacggcaa ccactccaac
    29701 cgtggcatta tcgccatgaa acaggtggtg ccgccgcagt tcgaggcgcg caacgacttt
    29761 gatattttcc gcgagctgtg ccgtcgcttt aatcgcgaag aagcctttac cgaagggctg
    29821 gacgaaatgg gctggctgaa acgcatctgg caggaaggtg tacaacaagg caaaggacgc
    29881 ggcgttcatc tgccagcgtt tgatgacttc tggaataaca aagagtacgt cgagtttgac
    29941 catccgcaga tgtttgttcg ccaccaggca ttccgcgaag atccggatct cgaaccgctg
    30001 ggcacgccga gtggcctgat tgagatctac tcgaaaacca tcgccgatat gaactacgac
    30061 gattgtcagg ggcatccgat gtggtttgag aaaatcgaac gctcccacgg tgggcccggc
    30121 tcgcagaagt atccgttgca tctgcaatct gtgcatccgg atttccgact tcactcgcag
    30181 ttatgtgagt cggaaactct gcgtcagcaa tatacggtag cgggtaaaga gccagtgttc
    30241 attaacccgc aggatgccag cgcgcgcggt attcgtaacg gtgatgtggt acgcgtcttt
    30301 aacgctcgcg gtcaggtgtt ggcaggggca gtagtttctg accgctatgc acccggcgtg
    30361 gcacgaattc acgaaggggc atggtacgat ccagataaag gcggcgagcc tggtgcgctg
    30421 tgcaaatacg gtaaccccaa cgtgctgacc atcgacatcg gtacttcgca gctggcgcag
    30481 gcgaccagtg cgcacactac gctggtggaa attgagaagt acaatggagc agtggagcag
    30541 gtgacggcgt ttaacggccc tgtggagatg gtggcgcagt gcgaatatgt tcccgcgtcg
    30601 caggtgaaat catgaccacg ctgacagcac aacagattgc ctgtgtttac gcctggctgg
    30661 cgcagttgtt ctcccgtgaa ctggacgatg aacaactgac gcaaatcgcc agtgcgcaga
    30721 tggctgaatg gttttcgttg ctgaaaagcg aaccgccgct ggctgcggcg gtgaatgagc
    30781 tggaaaactg tattgccacg ctgacagtac gtgacgatgc ccgtctggaa ctggccgctg
    30841 acttttgcgg cctgtttctg atgaccgaca aacaagcggc gctgccgtat gcatcggcct
    30901 acaaacagga cgagcaagag attaaacgct tgttagttga ggcagggatg gaaaccagcg
    30961 gcaatttcaa cgaaccggca gatcatctgg cgatctatct cgaattgctc agccatctgc
    31021 atttttcgct gggagagggg accgttcctg cgcgaagaat cgacagtttg cggcaaaaaa
    31081 cactgacggc gctgtggcaa tggttaccag agtttgttgg gcgttgtcgt cagtatgaca
    31141 gctttggttt ttacgcggca ctaagccagt tattgctggt gttagtggag agcgaccacc
    31201 aaaacagata acgtcgtttg tgcgcctgaa aagacgcgtt tagcgtcgca tcaggcatta
    31261 tggggcagtt gccggatgcg tcatcacatc cggcaaacac acatcacgga tgagttacaa
    31321 accgggaaag tcgctggcgc agcaggcggt tttcctgctt caggtgcgca atatcatcca
    31381 ttaacgtcag cgccaccgcg atcccaggcc agtccagagc cagttcatga cgcaggcgta
    31441 ctgcgcgttg caccacgatg gcggcatggt cgtcaaatac ccaggttgtt tcctggatct
    31501 cacgcggttc aaccaccccc aaaccgacaa tttcattcaa ctcctcttca gagatgccgg
    31561 tatgcaggca aaattcggta atagtaaaag tcaccgtaac attagccatt atgctttccc
    31621 ccaatcttta cgtggatcaa aagacgactg ggcgtctgcc agttgctgcc acagcgcggc
    31681 agtgttttca tccggtttcg gcggcatcac gattttcagt accgcataca gatcgccggt
    31741 ctgttttttg ctcaccagac ctttgccttt aacgcgcaat cgttgcccgg cctggctgcc
    31801 tggcgggata gtcagcaaaa tgctttcttt cagtgttgga acggtgactt tagcacccag
    31861 cgccgcttcc cacgggctaa ccggcactac aatttccaga tcatggccga caatatcgaa
    31921 cagcggatgt ggcgcaatat gaatcaccag ccacaaatcg ccatttggac cgccgttttc
    31981 gcccggcgtc ccctggcctt tcagacggat gcgttgacca ttgccgacgc ccgccgggat
    32041 cttgacattc agcgttttcg gaatttcctg ttcgatcatg ccaaaggcgt tataaaccgg
    32101 caggttatag ctgatggtac gcttatgctc agtaagcgtt tcttcgagga ataccgccac
    32161 ttcgatttca atatcgtggc cgcgtgtggc ggggcgttga cggctctggc gggcatgctg
    32221 accgaaaatt gacgagaaga tatcgtcaaa atcttcggcg ttaaaactct gaccgtcgct
    32281 atgatggaac tgacggttaa attgcggatc gttgcgatgt tgccacatct gatcatactc
    32341 agcgcgacgt tgttcatcac ttaacacttc ccaggcttca gcgacctctt tgaagcgggc
    32401 ttcggcatcc ggttctttgc tgacatcagg atggtatttg cgggccagtc gacgataggc
    32461 ggtcttgatt gtcttgagat cgtccgtcgg tttcacgccc atgatggcgt aataatcctt
    32521 taattccata gcgttatctc gcgtaaatca acacaaattg aaggaacccc tgtaaggtaa
    32581 ctcctataag tgtagggtaa tcctcaaaat ttcatatgcc aacacagaat atgttattga
    32641 aatcatcgcg gagaggaggt cgcctcaagg tggggtgctg aacatatttt aaacaggtga
    32701 aaaagggtga gcgatttttg atagtggaac caggcacttt aagtttaact ggggcgtcat
    32761 tatttattaa atttt
//
LOCUS       DAZFWL020000042        32531 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFWL020000042 DAZFWL020000000
VERSION     DAZFWL020000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.042"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..78
                     /locus_tag="ACHKNP_RS10540"
                     /old_locus_tag="pgaptmp_002108"
     CDS             <1..78
                     /locus_tag="ACHKNP_RS10540"
                     /old_locus_tag="pgaptmp_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704274.1"
                     /translation="LACGEMVQSGRSLKQEPTEMIQATA"
     gene            122..229
                     /locus_tag="ACHKNP_RS10545"
                     /old_locus_tag="pgaptmp_002109"
                     /pseudo
     CDS             122..229
                     /locus_tag="ACHKNP_RS10545"
                     /old_locus_tag="pgaptmp_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(213..851)
                     /gene="ytfB"
                     /locus_tag="ACHKNP_RS10550"
                     /old_locus_tag="pgaptmp_002110"
     CDS             complement(213..851)
                     /gene="ytfB"
                     /locus_tag="ACHKNP_RS10550"
                     /old_locus_tag="pgaptmp_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HGU4704275.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1070..1690
                     /gene="fklB"
                     /locus_tag="ACHKNP_RS10555"
                     /old_locus_tag="pgaptmp_002111"
     CDS             1070..1690
                     /gene="fklB"
                     /locus_tag="ACHKNP_RS10555"
                     /old_locus_tag="pgaptmp_002111"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4704276.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            1999..3402
                     /gene="cycA"
                     /locus_tag="ACHKNP_RS10560"
                     /old_locus_tag="pgaptmp_002112"
                     /gene_synonym="dagA"
     CDS             1999..3402
                     /gene="cycA"
                     /locus_tag="ACHKNP_RS10560"
                     /old_locus_tag="pgaptmp_002112"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HGU4704277.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(3444..3542)
                     /locus_tag="ACHKNP_RS10565"
                     /old_locus_tag="pgaptmp_002113"
                     /pseudo
     CDS             complement(3444..3542)
                     /locus_tag="ACHKNP_RS10565"
                     /old_locus_tag="pgaptmp_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3669..4097
                     /locus_tag="ACHKNP_RS10570"
                     /old_locus_tag="pgaptmp_002114"
                     /pseudo
     CDS             3669..4097
                     /locus_tag="ACHKNP_RS10570"
                     /old_locus_tag="pgaptmp_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4202..5269
                     /locus_tag="ACHKNP_RS10575"
                     /old_locus_tag="pgaptmp_002115"
     CDS             4202..5269
                     /locus_tag="ACHKNP_RS10575"
                     /old_locus_tag="pgaptmp_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704278.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDSVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(5516..6178)
                     /gene="ytfE"
                     /locus_tag="ACHKNP_RS10580"
                     /old_locus_tag="pgaptmp_002116"
     CDS             complement(5516..6178)
                     /gene="ytfE"
                     /locus_tag="ACHKNP_RS10580"
                     /old_locus_tag="pgaptmp_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HGU4704279.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6286..7251)
                     /gene="ytfF"
                     /locus_tag="ACHKNP_RS10585"
                     /old_locus_tag="pgaptmp_002117"
     CDS             complement(6286..7251)
                     /gene="ytfF"
                     /locus_tag="ACHKNP_RS10585"
                     /old_locus_tag="pgaptmp_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4704280.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7359..8219)
                     /gene="qorB"
                     /locus_tag="ACHKNP_RS10590"
                     /old_locus_tag="pgaptmp_002118"
     CDS             complement(7359..8219)
                     /gene="qorB"
                     /locus_tag="ACHKNP_RS10590"
                     /old_locus_tag="pgaptmp_002118"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU4704281.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            8308..8688
                     /gene="ytfH"
                     /locus_tag="ACHKNP_RS10595"
                     /old_locus_tag="pgaptmp_002119"
     CDS             8308..8688
                     /gene="ytfH"
                     /locus_tag="ACHKNP_RS10595"
                     /old_locus_tag="pgaptmp_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGU4704282.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8817..10760)
                     /gene="cpdB"
                     /locus_tag="ACHKNP_RS10600"
                     /old_locus_tag="pgaptmp_002120"
     CDS             complement(8817..10760)
                     /gene="cpdB"
                     /locus_tag="ACHKNP_RS10600"
                     /old_locus_tag="pgaptmp_002120"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HGU4704283.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            10950..11690
                     /gene="cysQ"
                     /locus_tag="ACHKNP_RS10605"
                     /old_locus_tag="pgaptmp_002121"
     CDS             10950..11690
                     /gene="cysQ"
                     /locus_tag="ACHKNP_RS10605"
                     /old_locus_tag="pgaptmp_002121"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HGU4704284.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(11680..12237)
                     /gene="ytfJ"
                     /locus_tag="ACHKNP_RS10610"
                     /old_locus_tag="pgaptmp_002122"
     CDS             complement(11680..12237)
                     /gene="ytfJ"
                     /locus_tag="ACHKNP_RS10610"
                     /old_locus_tag="pgaptmp_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU4704285.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            12562..12768
                     /gene="ytfK"
                     /locus_tag="ACHKNP_RS10615"
                     /old_locus_tag="pgaptmp_002123"
     CDS             12562..12768
                     /gene="ytfK"
                     /locus_tag="ACHKNP_RS10615"
                     /old_locus_tag="pgaptmp_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HGU4704286.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(12830..14173)
                     /gene="paeA"
                     /locus_tag="ACHKNP_RS10620"
                     /old_locus_tag="pgaptmp_002124"
     CDS             complement(12830..14173)
                     /gene="paeA"
                     /locus_tag="ACHKNP_RS10620"
                     /old_locus_tag="pgaptmp_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGU4704287.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14496..15134)
                     /gene="msrA"
                     /locus_tag="ACHKNP_RS10625"
                     /old_locus_tag="pgaptmp_002125"
     CDS             complement(14496..15134)
                     /gene="msrA"
                     /locus_tag="ACHKNP_RS10625"
                     /old_locus_tag="pgaptmp_002125"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HGU4704288.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            15340..17073
                     /gene="tamA"
                     /locus_tag="ACHKNP_RS10630"
                     /old_locus_tag="pgaptmp_002126"
     CDS             15340..17073
                     /gene="tamA"
                     /locus_tag="ACHKNP_RS10630"
                     /old_locus_tag="pgaptmp_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HGU4704289.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            17070..20849
                     /gene="tamB"
                     /locus_tag="ACHKNP_RS10635"
                     /old_locus_tag="pgaptmp_002127"
     CDS             17070..20849
                     /gene="tamB"
                     /locus_tag="ACHKNP_RS10635"
                     /old_locus_tag="pgaptmp_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HGU4704290.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTVQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     NRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            20852..21193
                     /gene="ytfP"
                     /locus_tag="ACHKNP_RS10640"
                     /old_locus_tag="pgaptmp_002128"
     CDS             20852..21193
                     /gene="ytfP"
                     /locus_tag="ACHKNP_RS10640"
                     /old_locus_tag="pgaptmp_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HGU4704291.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            21405..21656
                     /gene="chpS"
                     /locus_tag="ACHKNP_RS10645"
                     /old_locus_tag="pgaptmp_002129"
     CDS             21405..21656
                     /gene="chpS"
                     /locus_tag="ACHKNP_RS10645"
                     /old_locus_tag="pgaptmp_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HGU4704292.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            21650..22000
                     /gene="chpB"
                     /locus_tag="ACHKNP_RS10650"
                     /old_locus_tag="pgaptmp_002130"
     CDS             21650..22000
                     /gene="chpB"
                     /locus_tag="ACHKNP_RS10650"
                     /old_locus_tag="pgaptmp_002130"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HGU4704293.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(22080..22610)
                     /gene="ppa"
                     /locus_tag="ACHKNP_RS10655"
                     /old_locus_tag="pgaptmp_002131"
     CDS             complement(22080..22610)
                     /gene="ppa"
                     /locus_tag="ACHKNP_RS10655"
                     /old_locus_tag="pgaptmp_002131"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HGU4704294.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            22920..23876
                     /gene="ytfQ"
                     /locus_tag="ACHKNP_RS10660"
                     /old_locus_tag="pgaptmp_002132"
     CDS             22920..23876
                     /gene="ytfQ"
                     /locus_tag="ACHKNP_RS10660"
                     /old_locus_tag="pgaptmp_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HGU4704295.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            24016..25518
                     /gene="ytfR"
                     /locus_tag="ACHKNP_RS10665"
                     /old_locus_tag="pgaptmp_002133"
     CDS             24016..25518
                     /gene="ytfR"
                     /locus_tag="ACHKNP_RS10665"
                     /old_locus_tag="pgaptmp_002133"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HGU4704296.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            25532..26554
                     /gene="ytfT"
                     /locus_tag="ACHKNP_RS10670"
                     /old_locus_tag="pgaptmp_002134"
     CDS             25532..26554
                     /gene="ytfT"
                     /locus_tag="ACHKNP_RS10670"
                     /old_locus_tag="pgaptmp_002134"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HGU4704297.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            26541..27536
                     /gene="yjfF"
                     /locus_tag="ACHKNP_RS10675"
                     /old_locus_tag="pgaptmp_002135"
     CDS             26541..27536
                     /gene="yjfF"
                     /locus_tag="ACHKNP_RS10675"
                     /old_locus_tag="pgaptmp_002135"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HGU4704298.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(27569..28567)
                     /gene="fbp"
                     /locus_tag="ACHKNP_RS10680"
                     /old_locus_tag="pgaptmp_002136"
     CDS             complement(27569..28567)
                     /gene="fbp"
                     /locus_tag="ACHKNP_RS10680"
                     /old_locus_tag="pgaptmp_002136"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HGU4704299.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            28743..30116
                     /gene="mpl"
                     /locus_tag="ACHKNP_RS10685"
                     /old_locus_tag="pgaptmp_002137"
     CDS             28743..30116
                     /gene="mpl"
                     /locus_tag="ACHKNP_RS10685"
                     /old_locus_tag="pgaptmp_002137"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HGU4704300.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(30272..30823)
                     /gene="yjgA"
                     /locus_tag="ACHKNP_RS10690"
                     /old_locus_tag="pgaptmp_002138"
     CDS             complement(30272..30823)
                     /gene="yjgA"
                     /locus_tag="ACHKNP_RS10690"
                     /old_locus_tag="pgaptmp_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HGU4704301.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWHDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            30917..32269
                     /gene="pmbA"
                     /locus_tag="ACHKNP_RS10695"
                     /old_locus_tag="pgaptmp_002139"
     CDS             30917..32269
                     /gene="pmbA"
                     /locus_tag="ACHKNP_RS10695"
                     /old_locus_tag="pgaptmp_002139"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HGU4704302.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            32452..>32531
                     /locus_tag="ACHKNP_RS10700"
                     /old_locus_tag="pgaptmp_002140"
     CDS             32452..>32531
                     /locus_tag="ACHKNP_RS10700"
                     /old_locus_tag="pgaptmp_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="electron transport protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU4704303.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLE"
BASE COUNT         7703 a         8110 c         8881 g         7837 t
ORIGIN      
        1 cttgcctgtg gagagatggt gcagtcaggc cgctcgttga agcaggaacc caccgaaatg
       61 attcaggcga cagcctgaac gaagcaggga tccacgtcct tcagggcgtg gaggatgtca
      121 acatatcagt atggtttccc cccaggcact ggaagccaga cgtaaggtca tcaaagagac
      181 aatccttagc tggcttttgg ttgacccttc atctaccgcg cacgaataaa actgccatcc
      241 ggctggcggg taaacaggac ctgttgatta ttccccgtat caatggttaa gcccgtcacc
      301 acgccgctgg cgttctggcg gattttcacc atttgcccgt tttgcaaatt actcagcggc
      361 ttacccgcgc cttctacctg cgccattgca tagacatcgg tagcgggcag tccgtggtcg
      421 cggaacagct gcgccatcgt tttaccgggt tcgacacggt aagagcgcca ttgattatca
      481 attccgctat ctggctggaa tggttgcgtt tgggtagttt gcggctgctc ttctggctgg
      541 ccttcctgta taggttctgg agcgaccgga gccacctgat caggatcatt ttgtggggtg
      601 actaattgcg cccgcagctg ttcttctgtc ggcggttgtg attgcgactg aatatctaac
      661 tgcgcttcac gcgtcaccac cggcgcgttg ggcgtatcat cagatggaag cagaaatccg
      721 accaccaata caatggcggc aataatgatc ccccgacgat gcatcggcgg cagcgggtcc
      781 atgaagcgaa aattgtccgg cgcgtgccag actttctcca gggttggttt tagttcaaag
      841 cgcccgggca tggtttcctc ctgctccgcg tcttgttcct caatcatagc ctatgaataa
      901 gctaacgcta tgatgtccgt ggtaaacccg cctttattat attcatacgg gatattgcta
      961 ttgtttcttt ttccccggga tttgtcatca ttcctgcgac aaagttttac ccaaagaagt
     1021 gtggctgata tgctgcccgc tacttttata ccctaagaaa ggaaatacga tgaccacccc
     1081 aacttttgac accatcgaag cgcaagcaag ctacggcatt ggtttgcagg tagggcaaca
     1141 actgagtgaa tctggcctgg aagggctgct gccagaagca ctggttgcag gtattgccga
     1201 tgcgctggaa ggcaaacatc cggctgttcc ggttgatgtg gtgcatcgcg cgctgcgtga
     1261 aatccacgag cgcgccgatg ccgttcgtcg tcagcgtttc caggcgatgg ctgctgaagg
     1321 tgtgaaatac ctggaagaaa acgccaaaaa agaaggtgtg aatagcaccg aatctggcct
     1381 gcaattccgc gtgatcaacc agggtgaagg cgcaattccg gcacgtaccg accgcgttcg
     1441 tgttcattac accggtaaac tgatcgacgg caccgtgttt gacagctccg ttgctcgtgg
     1501 tgaacccgct gaatttccgg ttaatggcgt gatccctggc tggattgaag cactgactct
     1561 gatgccggta ggttctaaat gggaactgac tatcccgcag gaactggcat atggcgagcg
     1621 cggcgcaggc gcatccatcc ctccgttcag caccctggtg tttgaagtcg aactgctgga
     1681 aatcctctaa gcagcgcatt ctgttcccct cgaaccagag gggagcaggc attcagcaat
     1741 aaacccttca gtttgccaaa cggcgctatt ttgtgttgca aagaccccgt aagcgtgtat
     1801 ttttgtgagc tgtttcgcgt tatcaccgtg atatgacact cactttaaac ataaaattaa
     1861 cattagatct aaatcttagt attcatcccg cgtattgtta cctaatatcg atgagtcccg
     1921 atacagattc gtcgtatcat aggctgacta aaggccgtag agcctgaaca acacagacag
     1981 gtacaggaag aaaaaaacat ggtagatcag gtaaaagtcg ttgccgatga tcaggctccg
     2041 gctgaacagt cgctacggcg caatctcaca aaccgacata ttcagcttat tgccattggc
     2101 ggtgccattg gtacggggtt gtttatgggg tctggcaaaa cgattagcct tgccgggccg
     2161 tcgatcattt tcgtttatat gatcatcggt tttatgctct ttttcgtgat gcgggcaatg
     2221 ggggaattgc tgctttcgaa tctggaatac aaatctttta gtgacttcgc ttccgattta
     2281 ctcgggccgt gggcaggata tttcaccggc tggacttact ggttctgctg ggttgtaacc
     2341 ggtatggcag acgtggtggc gatcacggct tatgctcagt tctggttccc cgatctctcc
     2401 gactgggtcg cctcgctggc ggtgatagtg ctgctgctga cgctcaatct cgccaccgtg
     2461 aaaatgttcg gtgagatgga gttctggttt gcgatgatca aaatcgtcgc cattgtgtcg
     2521 ctgattgtcg tcggcctggt tatggtggcg atgcactttc agtcaccgac tggtgtggaa
     2581 gcgtcattcg cgcatttgtg gaatgatggc ggctggttcc cgaaaggttt aagtggcttc
     2641 tttgccggat tccagatagc ggttttcgct ttcgtaggga ttgagctggt aggtacaacc
     2701 gctgcggaaa ccaaagatcc ggagaaatca ctgccgcgtg caattaactc tattccgatc
     2761 cgtatcatta tgttctacgt cttcgcgctg attgtaatta tgtccgtgac gccgtggagt
     2821 tcggtagtcc cggagaaaag cccgtttgtt gaactgttcg tgttggtggg tttgcctgcg
     2881 gctgccagcg tgatcaactt tgtggtgctg acctctgcgg cgtcttccgc taacagcggc
     2941 gtcttctcta ccagccgtat gctgtttggc ctggcccagg aaggtgttgc accgaaagcg
     3001 ttcgctaaac tctctaagcg cgcagtaccc gcgaaagggc tgaccttctc ctgcatctgt
     3061 ctgctcggcg gcgtggtgat gctgtatgtg aatcccagcg tgattggcgc gttcacgatg
     3121 atcacgacgg tttccgcgat tctgtttatg ttcgtctgga cgattatcct ttgctcgtac
     3181 ctggtgtatc gcaaacagcg tcctcatctg catgagaagt cgatctacaa gatgccgctc
     3241 ggcaagctga tgtgctgggt atgtatggcg ttctttgtgt tcgtgatcgt gctgctgaca
     3301 ctggaagatg acactcgcca ggcgctgctg gttaccccgc tgtggtttat cgcgctgggg
     3361 ttgggctggc tgtttattgg taaaaaacgc atggcgaagt aatgccgcta aggcacctgc
     3421 gggtgtcttt caggctgcaa aattgcctga tgcgctacgc ttatcaggcc tacagaacct
     3481 ctgcaatata ttgagtttgc gtgcttttgt aggccgggta aggcgtttac gccgcatccg
     3541 gcatgaacaa agcacacttt gtcagcaatc tcctttgtct ttatacattc ctaaaaatac
     3601 ttcccgtcat cgacagctct caggcgcggg cttaccgtgg atctttgcca ctcattgcag
     3661 agttcactat ggctaagcac agttggttat taatcgccgc attacctctc tccatttcac
     3721 cttcctgggg cgcagatttt tattaccgcc agcaggagaa aggcacggtt tatgttgccg
     3781 aacagaaggg agaaaaggat gagatcctct ccgaactgcc gggcgttaat ttttcccgcc
     3841 tctggcgtat tgccaattta gccaataacc aagaaacccg cttactgtcc gattttaatc
     3901 ccgataagtt cgattgcgat gatcggaata attgccagca tacctggctc accgatggac
     3961 gctctgttct ttggtctggc aaagtcctga aaaatccgcc cggtgaacct aatgtcgatg
     4021 ccgccagttt tcaggcattc ggtgctttcg ctgccgataa acgcagcgtc tattttgacg
     4081 gtcagcgtac cgatgataga tgaagtggat atgtatatag ctgtgtctca gttgcaaaca
     4141 aatatttatg taccgaaata aaagtatcaa tagacaatta attaataaat caggagataa
     4201 agtggctata ttacatccac aggagtgctg gttgctggaa agaataatgt cgccggaata
     4261 ttatcggcgt cgttttgagg gctggcagga gtttgtcgag ctgtgcgagc gacaggtggc
     4321 agagtggagc aagaccatgc cgctggatgt gcgccgtcgt ccgctgtgcg agcagattga
     4381 cgtcgtctgg ggcgggcgag tgttgcccaa tatccgcagt actttaaagt cggtacagta
     4441 tgactttatt cagctacagc agggggacct gcgggtactg caatccgggg gcaatattag
     4501 cagtgatatg aaaggactaa ttgattatcc gtcggactgg atgtcactcg ccgcgcaaaa
     4561 acagtatgac agactgaaat ggcgaggtgc acattataat aatttaatca gacgtacctc
     4621 gggcggatac tggtatgacg gcgaactgac ttattattat gaggaaagtc tgcacggccc
     4681 gctggccctg ccgatgcaat taccgttgta tgagctggat agtagcgtat acctgcgtga
     4741 ggatgactcg gtaacggtgg ctggcctgta tctgccggat atcccagatg ccagcgccca
     4801 actgttgtac cggagtgaac atattcccga ggcttggcag gggcgggtac gaacgaagta
     4861 tgtcaacgag gctgggatac aagaatatta ctgggaaagc ggggcgtggg caaaatgcaa
     4921 ttggaaacgt atacggcggg tggcaaaccg atttatcaat gtgccgccgg agggattttt
     4981 cccacagggc atgccggagg agttgtacaa ctggccacag cgcgaagcgc agtatgttac
     5041 agaccggcag cggatagcgg cctttagcgg tgaagcttgt cctcatagcg gggagtggtc
     5101 gatttttgta gaaggtagac aagcgacggt gacactggag cagggagagc agatgccaga
     5161 atggacggac aggaaaatgg agggggagta taaaagaggc gagaaattcc atgtgctgtg
     5221 gtcgctgatg aaccgccatg atggcgggtc ggtgtgggtg gaagcgtagc ggtagctgga
     5281 tgtacacgta aaaaccaggg aatgggtgat tttattctgg cttatacagg agcgatgaat
     5341 aagtaattat attaatgaaa aaaataagtt agtaaccgtt gaaggaactt attattggct
     5401 gaataatgaa ttttcccagt tcagtagata caaaagattg tcccggagtg gtgaataact
     5461 caaagcattt gaaaacgcac ctgattgtgt aaaccaggtg cgttgatcga acaaattact
     5521 cacccgccag cgcgcgtggg aacagtacat tgttttccag gctgatgtga tccatcaggt
     5581 catcaatcag ttcattaata ccgttataca tcgctttcca ggtggtgcag gcttccggcg
     5641 gcggtgtgac gttattggtg gtgtgtttaa tcacttccag cagttcgccc gcttcatcgt
     5701 gctcgctttc catcacgctg attggtccca ttgcctggct gcccatgcct tgtttgatca
     5761 tcgggaagag gatctgctct tctttcatca tgtggctgga aagctcttca tgcagcatag
     5821 tcaggtattt tgtcagccct tttggcacgc tcggtttgtc ggcgtgaacg cgctcgactt
     5881 tagtcgcttg caggatcagc tccggcagct gctcgcggtg acgatcgtgg tagcgcacga
     5941 tgatatggtc aatgatttct gccagcgggg cgctacgcca gtctttctca atcggttgtt
     6001 cagcaagctt tgccagttca gcttcaatga cctcaacatc cagttcttta cgtgccgccg
     6061 cgcgcgccag cgtctgctta ccgccacagc agtaatccat atcatattta cgaaacagag
     6121 ccgaagcgcg agggatagag agcgccagtt cacctaaagg ttggtcgcga taagccatag
     6181 ctgatacctc attcttaata agataagatg tattttaaat gcatctttaa ggcaaaagct
     6241 ataaccctta cgtagcagag gcttatttaa ctcactgcca gcgtgtcaac tttctgagac
     6301 agattcagtt aaaggttttt ctggttttgc tctgaccgca atgaccacgc caatcactaa
     6361 cagcgcgata ccgctcagcg tcattagcgg cggcatttgc tggcggagta aaaaggtgta
     6421 cagcaaacct gccagcgttt cgaaaacaat cagcggcccg agaatcactg tcggtaatcg
     6481 ctggctggcg acgttccagc agagtgcgcc aacccatgag caaagcacgg ctatcgcaac
     6541 catcagacta ataaacacca gcggacgggg gccaaaaggt aaggagaagt ccggcgtttg
     6601 tatattcagc cagtaacagg cgacgagata gccgatgaga gaaaccggca gcgtgaccag
     6661 cgcctgcgcc gtcgcccaca tcatcggatg tttgtcggga ttttcccgca gccagcgggc
     6721 gttgcgcagg gcataccatg cccagcagac cacggaaact aacgctagca cgatgccaga
     6781 ggtataacgt gcccagtcaa aatcggggag tccgtggttt aactcagcaa tattcacact
     6841 cgccaggccg atgccaatac aaatcagtgc cggggcgagt tttccccacg cgagtttgcc
     6901 gtcgcgctgg ctataaagca gattggcaaa gacaggaatg accaccggca gggtgccgat
     6961 aatcatcgtg gaaacaggcg cgccagtacg ttgaatggca ctggcaaggc agaagtaata
     7021 aatgaggttg cccatcatag tgagcatcaa ggcggtaagc cagtcccgac gcgccaactg
     7081 acgcagacgc acgcgtccca gccaggcaat gggcagcgca attaacccta acgccagata
     7141 acgccccatc gactgcaaca tcgccgggta ttccggtacg atcaacggcc cgacaaaaat
     7201 aagcccccac atcaaccctg ctaacagggc gtacagcacg ccgctaatca ttactggcat
     7261 ccattggtct gtcagaagaa agttcaggaa gcagacatga ttgctcccgg catcccgtca
     7321 ttattagaga ggatgcggga ggatgccact ttaattaact agttattaac attaaaaaga
     7381 tggcttacgc tttcggctaa cgttgtcgtt gggcggccaa tcaatttgct aagcgttttg
     7441 ctgttatcaa acagaccacc tttcgatgcg ccaacgtcag aatccgccag catatccgcc
     7501 agtccgtcgg gcagtccgac gcttttcagt gccgcggcga aatcggcttc gctcagattt
     7561 tgataggtaa cctgtttgcc gctctgtttg gtcagctctg ccgctaactg tgtcaatgtc
     7621 caggcactat cgcccgccag ttcgtaaacc ttgccttcgt gaccggcttc gctaatcacg
     7681 cgtgccgcag ctgccgcata atctgcccgc gttgctgagg cgattttgcc atcgcccgcc
     7741 gcaccgataa atacgccgtg ttccagtgct gccggggcgc tggcgaggta gttttcgctg
     7801 taccagccgt tgcgcagcag ggtgtaaacg atgccagaat cagccaacat tttctccgtc
     7861 tcgatgtgct catcggcgag gccgagcggg gaggtatctg catgtagcag gctggtataa
     7921 gcgataaatt tcacaccagc cgcctttgcg gcattaataa cattacgatg ctgcggggca
     7981 cgttgaccca cttcgctgga agagatcagc agtagttttt ccactccctg aagtgcagat
     8041 gtcagtgcgg cttcatcgcc gtagtcagcc tgacgcacgg taatgccttg tgctgccaga
     8101 gcctgggctt ttgccggatt acgaacgata gccactattt ggctgacagg aaccgttttc
     8161 ttcaaggatt caataacata gtgaccaagt tggccagtgg caccagtaat agcgatcatg
     8221 ggaagtctcc atcgttttgt cttgtgttta aatacgctaa cacctaaact tacttttagt
     8281 aagtacgcac aaaaaggtaa gtatgaaatg agtcaggtta gcctgtcgca acaactgaaa
     8341 gagggtaatc tctttgcgga acagtgcccg tcgcgcgagg tgttgaaaca cgtcaccagc
     8401 cgttgggggg tgttgattct ggtggcgcta cgcgaaggta ctcatcgctt tagcgacctg
     8461 cggcgcaaaa tcggcggggt gagcgaaaag atgcttgcgc agtcgttaca ggcgttagaa
     8521 caggatggtt ttcttaaccg tatcgcgtat ccggtggtgc cgccgcatgt ggaatatagc
     8581 ctcacgccgc tgggcgagca ggtgagcgaa aaggttgctg cactggcgga ctggattgag
     8641 ttgaatttgc ccgaggtgtt ggcggtgcgg gatgaacgtg cggcataact gccggatgcg
     8701 ctgagcttat ctggtctacg gtgcatggct tgtagggcgg ataagatgcg ccagcatcgc
     8761 atccgccaat aaatgccgga tgcggttgat gatttatagg ccgaaaaaga agtgttttac
     8821 ttactcaaat ccacctgata aatcgcaaac ccgatatcat cggtcgcgac tttattcatc
     8881 ggatattgcc ctttctcttt aataaacgct gcggctttat ctgacggaga agtttcgaaa
     8941 cggatatcca gtttcttatc gccagctatc ggtgctaaac gccagttgtt atctgccgcc
     9001 gggtgaattt cccccgcacg tttcgactca tcagcaatcc acgctgccag caccgagcgg
     9061 ttctcatccg gtgaagcaaa agcgatatgg ctgtcgcccg taccggcaaa tttgccgccg
     9121 taagcgcgat agttattggt ggcaacgagg aacatggcgt tcggatcaat cggcttgcca
     9181 ttaaaggtca ggttcttaat cctttccgca ttggcattaa tcatctggca ctcgccgtca
     9241 taacgggcag gctgggtaac atcaatctga taattcacac catcaatcac atcaaagtta
     9301 taggtgcgga aaccatccca gttgatgagt gattgcggtt tcgtgctgtt aggatcaatc
     9361 tggttaaact gtccggcgga gcactccagc cactctttca cctctttacc gctggctttc
     9421 accacaatca gcgtattggg gtagagataa agatcggcgg cattacggaa ggtcaactgg
     9481 cctttttcca cctccacata gcttgccggg tcatttttgc gaccaccaac tttaaacggt
     9541 gcggcagctg aaagcaccgg cagttttgcc agatccggat cgccctgaat gtaatgttcg
     9601 acatacgctt tttgcgcgtt gttcactact tgcacggtcg gatcgtcctg caccagcgcc
     9661 agatagctat acatattgtc ggcagattta ccgattggct tgctgacgaa ctggcgtgtg
     9721 gcatcgtgat cggctttgag tgtttctacc agcttgctgt cttccgccgc gagggatttt
     9781 ttattggcga tgtcgtaaat cggccgagct tccgctttcg cctgcgtcac ctgccattta
     9841 ccgctgtcat tactgagttg taagtcgacc accccaagat gatcgcccca catgcccggc
     9901 attaccgccg gaacaccatt cagcgtgcct ttggtgatat cagccccttc gatatcagca
     9961 aaatctttac ccgggaaaac ggcgtgagca tgaccaaaca taatggcgtt aacgcccgga
    10021 atttcactga ggtaataaac tgagttttcc gccattactt tatacggatc ggcagacagc
    10081 ccggaatgcg ccagaacgac aacgacatcg gcacctttct cgcgcatttc aggcacgtat
    10141 ttgcgcacgg tttcggtaat atcattcacc gtcacttttc cggataaatt agctttatcc
    10201 cagcccatga tttgcggcgg cacgacgcca atatagccaa tcttcagcgt ctgttttttt
    10261 ccgtctttat cgaccacttc ggtatcttta attaaatacg gtgtaaacat tggctgtttg
    10321 gttctggcgt caatgacgtt ggcatttaca taagggaatt tcgctcccgc cagcgcattt
    10381 ttcaggtaat ccagaccgta gttaaattca tgattgccga gtgtaccgac cgtataatcc
    10441 agcgtattca gcgccttata aaccggatga acatcacctg cttttaatcc tttcgccgac
    10501 atgtaatcgg ccagcggact cccctgaatc aaatcgccgt tatcgaccag tacgctgttt
    10561 ttcacttcat tgcgggcatc gttaatcagg cttgccgtac gtaccagtcc gaatttttcc
    10621 gtggcggtgt ctttgtaata atcgaaatcc atcatgttgc tatgcagatc agtggtttcc
    10681 atgatacgca gatcgaccgt cgctgcattc acactggcgg caatcagcgt ggccaggagc
    10741 gttgcgctaa acttaatcat cagggacatc cttttatcat cgggaatacg aaagaaaagg
    10801 gagaataaac gtcttactta tagaacagtg aagaatgcca caattttacg ctttgaaaat
    10861 gatgacacta tcacagttgg cgcattcatt aacgataggg tataagtaaa acaataagtt
    10921 aacaccgctc acagagacga ggtggagaaa tgttagatca agtatgccag cttgcacgga
    10981 atgcaggcga tgccattatg caggtctacg acgggacgaa accgatggac gtcgtcagca
    11041 aagcggacaa ttctccggta acggcagcgg atattgccgc tcacaccgtt atcatggacg
    11101 gtttacgtac gctgacaccg gatattccgg tcctttctga agaagatcct cccggttggg
    11161 aagtccgtca gcactggcag cgttactggc tggtagaccc gctggatggt actaaagagt
    11221 ttattaaacg caatggcgaa ttcaccgtta acattgcgct cattgaccat ggcaaaccga
    11281 ttttaggtgt ggtgtatgcg ccggtaatga acgtaatgta cagcgcggca gaaggcaagg
    11341 cctggaaaga agagtgcggt gtgcgcaagc agattcaggt ccgcgatgcg cgcccgccgc
    11401 tggtggttat cagccgttcc catgcagatg cggagctgaa agagtatctg caacagcttg
    11461 gcgaacatca gaccacgtcc atcggctctt cgctgaaatt ctgcctggtg gcggaaggac
    11521 aggcgcagct gtacccgcgc ttcggaccaa cgaatatttg ggacaccgcc gctggacatg
    11581 ctgtggccgc agctgccgga gcgcacgttc acgactggca gggtaaaccg ctggattaca
    11641 ctccgcgcga gtcgttcctg aatcctgggt tcagagtgtc tatttattaa ttaatttatg
    11701 cagcaggtcc atcacttgct gcacctcttc ctgagtaagc gccccgtctt tggcccattg
    11761 cacgcgaccg tctttatcca gcaccactac agcggaactt ttttcatcca gctgccaggc
    11821 accgcgtgcg acgccattgc tatcaacaat aaactgcgac cagggataaa gctttttatt
    11881 actctccaga ctgctgcgca caaacatgcc ggaacccgga attgcgtcgt cggtgttaac
    11941 aatggtggtg gtctggtaac gatcgtgcgg taacttcgct gatttaatcg cttcaatcag
    12001 cgtcgcgttt ttctcttttg cagaggtgcg accagcaata tgttgcagta ctcgcacttt
    12061 tcccactaac tgcgcgctgt tccaggtttt gtagctaaac tgatctttat caagcaccaa
    12121 ctcgccacga tcggtgatgc caatcggcgg cactcgctga ccggtttcga actgatgtgc
    12181 ggaagccatc atcggcaaca gcaggcaggt gagtgccaga atcttgcgta gggtcatggt
    12241 gtttccttct tatgatatgc aggtgatccg accacttggg tctgagtttt aatcatatgt
    12301 gctatttatc gaatttcccg caagtgtgat gccagtttgc ggtcaagcgc acaaatcata
    12361 tgaaaaatga atgcttatac tgaagaccgc gcttcggtaa aaagataatt ctgaataatt
    12421 gtaaccttta ggtaaaaaaa gttatacgcg gtggaaacat tgcccggata gtctatagtc
    12481 actaggcatt aaaatttgcg cctcataata gttgggccga ttgtggcacc gcacaggcgt
    12541 aatactcagc aggagataac aatgaaaatt ttccaacggt acaacccact tcaggtggcg
    12601 aagtacgtaa agatcctgtt ccgtggacgg ttatacatca aggacgttgg cgcttttgaa
    12661 tttgataagg gtaagattct tatcccaaaa gtgaaggata agctgcattt gtcagtgatg
    12721 tccgaagtta accgtcaggt tatgcgtctg caaacagaga tggcttaacc aaagtgctat
    12781 gcagtaataa gacggctcct gattcaggag ccgttgatgt ttctgggggt tacgcgacgc
    12841 tttcttcttt atctttagcg tcaggcagtt ttggcgaaag ggcggtggcc ttgctgtcga
    12901 tccgggtcac cagcagctgg tcgatgcggt agttatcgat atccaccact tcaaatttgt
    12961 agccggcgaa tttcaccgaa tcggtgcgtt tcgggatctt acgcagcata aacatcataa
    13021 agcctccgat ggtttcgtag ttgcccgact gcgggaactc gtcaatatcc agcacgcgca
    13081 tgacgtcgtc aattggggtg ccgccgtcaa tcagccatga gttctcatca cgggcgacaa
    13141 tctgctcttc cagcccctga ccgaccagat cgcccatcag cgtggtcatc acgtcgttga
    13201 gggtgatgat ccccaccacc agcgcgtact cgttcatgat caccgcgaag tcttcacctg
    13261 cggttttaaa actttccaac gcctctgaaa gggttaacgt atccggcaca atcagcgtgt
    13321 tgcgaatttg tacgccgctg ttcagtgcca ggctttggtt agccagcacg cggttcagca
    13381 ggtctttaga atcgacataa ccgatgatgt ggtcaatatc ttcattacag acgaggaact
    13441 tagagtgcgg atgttccgcc accttattct tcaggctttg ctcatcttcg tggagatcaa
    13501 accaaatcac gttttcacgc ggtgtcattg aagacggaac ggtacgggat tccagctcaa
    13561 agacgttttc aatcagctcg tgttcctgtt tacgtaacac gcccgccagc gcaccggctt
    13621 ccactaccgc gtagatgtca tcagaagtga tgtcatcttt acgtaccatt ggcagtttga
    13681 aaatacggaa gatcatgttc gccaggccgt tgaagaacca caccagcggg gtgcaaacgt
    13741 acaggcagaa gcgcatcggg ttgatgatac gcaaagccac cgcttctggc gcaatcatac
    13801 cgatgcgttt cggggttaaa tccgcaaaca ggataaacat gccagtcact aacgagaaag
    13861 agagaataaa gctcagttgc tcagagagtt ctgccgacat atagcgggag aacaggctgt
    13921 gaaaagctgg agaaaatgcc gcatcaccga cgataccgcc gagaatcgca actgcgttca
    13981 gaccgatttg gaccaccgta aagaacatgc cgggattttc ctgcatattc agaacgcgtt
    14041 gggcatttat attgccttca tcagccagca gtttaagttt gattttgcgt gaggcggcaa
    14101 gtgagatctc agacatcgag aagaacgcac ttacagcgat caagcagagt atgactaaaa
    14161 tactgtttaa catatcttat ccgacctttc aggccggatc ctcggaaggg gaagttgatt
    14221 aatttgtgtg aaatacacat tgaaagccga ttcgaagaaa gtgaaccggc attttcagtg
    14281 gctagtatag cgtaaggtac tgtaaagtcg ccagagggtt aaaattcaga ctgctgataa
    14341 atgcaatact gcctgatgcg ctatgccgat caggcctacg cagtccctgc aatatattga
    14401 atttggacga ttttgtaggc ctggtaaggc attcacgccg cattcggcat aaacaaaacg
    14461 cactttatca acaatctaca tttgtacccg taacgctatg cttccggtgg cagacagacg
    14521 ccaattccgc caattccaca gtaaccatac gggtttttat gcagatattg ctggtggtca
    14581 tcttcggcat aataaaacgg tgtggcgtta gcgatttccg tggtgatgtg acgatcgtca
    14641 tcggcggcaa gcatcgccgc ctgaaaacgt tccagactgg cgcgagctgc ggcatcctgt
    14701 tctggggtca gcggataaat cgctgaacga tactgcgtgc cgtggtcatt gccctgacgc
    14761 attccctggg cgggatcgtg attctcccaa aatacctgta gcaactgctc atagctgatg
    14821 acagaaggat cgtaaacaat gcgtaccgct tcggcatgac ccgtatcacc ggagcacact
    14881 tcccgataag tcggatttgg cgtatagccg ccggtatagc ctgcggcggt gctgtaaacg
    14941 ccgggtaact gccagaacag acgctccaca ccccagaaac aacccatcgc aaaaatggca
    15001 atctccattc cgtcaggtac attggtcatt gagtgaccgt tgaccgcatg cagcgtggct
    15061 acgggcatcg gggtgttacg tccaggcagg gcatcggcgg gggaaaccag atgcttttta
    15121 tcaaataaac tcatggtgtc gctctcccga aaatcggtca ttggggttaa ggttgtaaca
    15181 agaggcgtat ttgcacacaa taaccaccgt gaataggtct aaagtaaaac ataagaaata
    15241 tttggggttt agtctgcttt ttaatccata ttactggatt tttgttaagc cgtttaacgg
    15301 cgttccaggg gcaggaaaaa aggatattca ggagaaaatg tgcgctatat ccgacagtta
    15361 tgctgtgtaa gcttactctg cttaagcgga tctgccgtcg ccgcgaacgt ccgtctacag
    15421 gtcgaggggt tatcgggaca gctggaaaag aacgttcgtg cgcagctttc tacgattgaa
    15481 agtgatgaag tgacgccaga ccgtcgcttt cgcgcacgcg tcgatgatgc catccgcgaa
    15541 ggtctgaaag cgctgggtta ttaccagccg accattgaat ttgatctccg tccaccgcca
    15601 aagaaagggc ggcaggtatt gatcgccaaa gtcacgccag gcgtgccggt gttaattggc
    15661 ggcaccgatg tggtattgcg cggcggcgcg cggaccgata aagactattt gaaattgctc
    15721 gatactcgcc cggctattgg cacggtactg aaccagggcg attatgaaaa tttcaaaaag
    15781 tccttaacca gcattgcgtt gcgtaaaggt tatttcgata gcgaatttac caaagcgcag
    15841 ctgggcattg cgctcggcct gcataaagcc ttctgggata ttgattataa cagtggcgaa
    15901 cgttaccgct ttgggcatgt gacctttgaa ggatcacaaa tccgcgatga atacctgcaa
    15961 aatctggtgc cgtttaaaga gggcgatgag tacgaatcga aagatctggc ggaacttaac
    16021 cgtcgacttt ctgctaccgg ctggtttaac tcggtcgtag tggctccaca atttgataaa
    16081 gcgcgcgaaa cgaaagtatt acccttgacg ggcgtggttt cgccgcgaac tgaaaacacc
    16141 atcgaaaccg gggtcggtta ctctacggac gtgggaccgc gcgtgaaagc tacgtggaaa
    16201 aagccgtgga tgaactctta tggtcacagt ctgaccacca gtaccagtat ttccgcgccg
    16261 gaacagaccc tcgacttcag ctataaaatg ccgctgctga agaatccact ggaacaatat
    16321 tatttggtgc agggcggttt taagcgcact gacctgaacg ataccgaatc tgactccact
    16381 acgctggtgg cttctcgcta ctgggatctc tccagcggct ggcagcgtgc cattaacctg
    16441 cgctggagtc tcgaccactt tactcagggt gaaattacca acaccacgat gctgttttat
    16501 cctggggtga tgattagccg cacgcgttct cgtggtggcc tgatgccaac ctggggcgac
    16561 tcgcaacgct actctatcga ctactccaac acggcctggg gctcagatgt cgatttctcc
    16621 gttttccagg cgcagaacgt ctggatccgc acactgtacg atcgccatcg tttcgttaca
    16681 cgcggcacgc tgggctggat tgaaaccggt gatttcgaca aagtaccgcc ggatctgcgt
    16741 tttttcgccg ggggcgatcg cagtattcgc ggctacaaat acaaatctat cgccccgaaa
    16801 tacgccaacg gtgacctgaa aggggcctcg aagttgataa ccggatcgct ggaataccag
    16861 tacaacgtga ccggaaaatg gtggggcgcg gtgtttgtcg atagtggcga agcggtaagc
    16921 gatattcgcc gcagcgactt taaaaccggt accggggtcg gcgtgcgctg ggaatcgccg
    16981 gtcgggccaa tcaaactcga ttttgccgta ccggtcgcgg ataaagacga acacgggtta
    17041 cagttttaca tcggtctggg gccagaatta tgagtttatg gaaaaaaatc agcctcggcg
    17101 tggttatcgt tatcttactg ttgctgggat cggtggcgtt tctggtgggc accaccagcg
    17161 gcctgcatct tgtatttaaa gcggcggatc gctgggtgcc aggactggat attggcaagg
    17221 tcaccggcgg ctggcgcgat ctcaccttgt ctgacgttcg ttatgagcag ccaggcgtgg
    17281 cggtaaaagc gggtaatctg catctggcgg tcggtcttga gtgcctgtgg aacagtagcg
    17341 tttgtattaa tgacctggcg ctgaaagaca ttcaggtcaa catcgacagt aaaaaaatgc
    17401 ctccttctga acaggttgaa gaagaggaag atagcggtcc gctggatctc tccacgccgt
    17461 atcccatcac cctgacacgg gtggcgctgg acaacgtcaa catcaagatt gatgacacca
    17521 cggtgtcggt gatggacttc acctccggcc tgaactggca ggagaaaacc ctgaccctga
    17581 aaccgacgtc gctgaaaggc ctgctgattg ctctgccgaa agtggcggaa gtggcgcagg
    17641 aagaagtggt cgaaccgaaa attgaaaatc cgcagccgga ggaaaagccg ctcggcgaaa
    17701 cgctgaaaga tctcttttct cgcccggtat tgccggaaat gaccgacttg catttgccgc
    17761 ttaacctgaa cattgaagag tttaaaggcg agcagttacg cgtgacgggc gacacggaca
    17821 tcaccgtgcg caccatgctg ctgaaagtga gcagcattga cggcaatact aaactggacg
    17881 ccctggatat cgattccagt caagggatcg tcaacgccag cggcacggcg cagctgtcag
    17941 acaactggcc ggtggatatc accctcaaca gcacactgaa cgtggagccg ttgaaaggtg
    18001 aaaaagtgaa gctaaaagtg ggcggcgcgc tgcgcgaaca gctggagatt ggcgttaatc
    18061 tttccggtcc ggtggatatg gatttacgcg cccagacgcg actggcggaa gccggattgc
    18121 cactcaacgt ggaagtgaac agcaaacagc tttactggcc gttcaccggt gagaagcagt
    18181 atcaggcgga tgatctgaaa ctgaaactta ccggcaaaat gaccgattac acgctctcta
    18241 tgcgtacggc agtgaaggga ctggagatcc cgccagccac cattaccctt gatgccaaag
    18301 gtaatgaaca gcaggtcaat ctcgacaaac tcaccgtcgc ggcgctggaa gggaaaactg
    18361 aactcaaggc gttgctcgac tggcagcagg ccattagttg gcgcggtgag ctaacgctta
    18421 acggcattaa caccgccaaa gagatcccag agtggccgtc gaaactcaat ggcttgatta
    18481 aaacccgcgg tagcctgtac ggcggcacct ggcagatgga ggtgccagaa ctgaagctga
    18541 ccggtaacgt caaacagaac aaagtgaacg ttgacggcac gctgaaaggc aacagttata
    18601 tgcagtggat gatcccaggg cttcatctgg aactggggcc aaacagtgcc gaagtgaaag
    18661 gcgagctggg ggtaaaagat ctcaatcttg atgccaccat caacgcgccg gggctggata
    18721 acgcgctgcc ggggcttggc ggtacagcga aagggctggt gaaagttcgc ggcacggtgg
    18781 aagcgccaca actactggcg gacatcaccg cgcgcggcct gcgctggcag gagctaaccg
    18841 tggcgcaggt tcgcgtggaa ggcgatatca aatccaccgt tcagattgcg ggtaaactcg
    18901 acgtgcgcgt tgagcaaatt tcacagccgg atgtaaatat caacctcgtc accctgaatg
    18961 ccaaaggcag cgaaaaacag cacgagttac agttgcggat tcagggcgag ccggtttccg
    19021 ggcagcttaa tctggcagga agttttgatc gcaaagaaga acgctggaag ggaactctta
    19081 gcaatacccg cttccagacg ccggtcggcc cgtggtcgct gacccgcgat attgcgctgg
    19141 attaccgcaa taaggagcaa aaaatcagca tcgggccaca ctgctggctt aacccgaatg
    19201 cggaactgtg cgtgccgcaa actatcgatg cgggggccga agggcgtgcg gtggtgaatc
    19261 tcaaccgctt cgacctcgcc atgctgaaac cgtttatgcc agaaaccact caggccagcg
    19321 gtatcttcac gggtaaagcg gatgttgcct gggacaccac gaaagagggg ctgccgcagg
    19381 gcagtatcac cctttcgggg cgtaacgtgc aggtaacgca aaccgtcaac gatgcggcgc
    19441 tgcccgtggc gtttcagaca ctgaatctga cggcggaatt gcgtaacaac cgtgccgaat
    19501 tgggctggac catccgcctg accaataacg gccagtttga tggacaggtg caggtgaccg
    19561 atccgcaagg ccgccgtaat cttggtggca acgtcaatat ccgtaacttc aaccttgcga
    19621 tgataaaccc catctttaac cgtggggaaa aagcagcggg gatggtgagt gccaacttgc
    19681 gtctgggtgg tgatgtgcaa agcccgcagt tgtttggtca gcttcaggtt acgggtgtgg
    19741 atatcgacgg caactttatg ccgtttgata tgcaaccgag ccagcttgcg gtcaacttta
    19801 atggtatgcg ctcgacgctt gccggtacag tacggaccca gcagggtgaa atctacctga
    19861 acggtgatgc cgactggagc caaattgaaa actggcgggc gcgggtaacg gcgaaaggca
    19921 gtaaagtgcg gatcaccgtg ccgccgatgg tacgaatgga tgtatcgcca gatgttgtct
    19981 tcgaggctac accaaacctg tttaccctcg atggtcgcgt ggatgtcccg tgggcgcgca
    20041 tcgtggtgca cgatctgccg gaaagcgcag taggcgtctc cagcgatgtg gtgatgctta
    20101 acgataacct gcaacctgaa gagccgaaaa cggcgtcgat tccgattaac agtaacctga
    20161 ttgtccacgt tggcaacaat gtgcgcattg acgcctttgg cctgaaagcg cggctgacgg
    20221 gcgatcttaa cgtcgttcag gacaaacaag ggctgggcct gaacgggcag atcaacatcc
    20281 ctgaagggcg cttccatgcc tatggtcagg atctgattgt gcgtaaaggt gagttactgt
    20341 tctctggtcc gccggatcaa ccgtatctta atattgaagc tattcgtaac ccggatgcta
    20401 cagaagacga cgtaatcgcc ggagttcgcg tcactggtct ggcggacgaa ccgaaagcgg
    20461 agatcttctc tgacccggcg atgtcgcaac aagctgcatt gtcttatttg ctacgtggac
    20521 aagggctgga gagcgatcag agcgacagtg cggcaatgac ctcgatgctg attggtctgg
    20581 gggttgcgca aagtggccag attgtgggta aaatcggcga gacgtttggc gtaagcaatt
    20641 tagcgctcga cacccaggga gtaggcgact cctcccaggt agtggtcagc ggctatgtat
    20701 tgccaggtct gcaagtgaaa tatggcgtgg gtatatttga ctctatagca acactcacgt
    20761 tacgttatcg cctgatgcct aagctatatc tggaagccgt gtctggtgta gaccaggcac
    20821 tggatttgct ctatcagttc gagttttagc aatgcgaata tttgtctacg gcagtttacg
    20881 ccacaaacaa ggcaacagtc actggatgac caatgcccag ttactgggcg atttcagtat
    20941 cgataactac cagttgtata gcctgggcca ctatccaggc gcagttccgg ggaacggaac
    21001 agtacacggt gaagtttatc gtattgacaa cgccacgctg gccgaacttg atgccttgcg
    21061 caccaggggc ggtgaatacg cgcgccagtt gattcagacg ccgtacggga gtgcatggat
    21121 gtacgtttat caacgacccg tcgatggatt aaagctaatt gaaagcggcg actggttaga
    21181 cagggataag taaccatatg catacgccac cttcgggtgg cgttgttttt tgcgagacga
    21241 ctcgcattct gttttgtaat tccctcaccc tttgcttttc tctccgagcc gctttccata
    21301 tctgttaacg cataaaaaac tctgctggcg ttcacaaatg cgcaggggta aaacgtttcc
    21361 tgtagcaccg tgagttatac tttgtataac ttaaggaggt gcagatgcgt attaccataa
    21421 aaagatgggg gaacagtgca ggtatggtca ttcccaatat cgtaatgaaa gaacttaact
    21481 tacagccggg gcagagcgtg gaagcgcagg tgagcaacaa ccaactgatt ctgacaccca
    21541 tctccaggcg ctactcgctt gatgaactgc tggcacagtg tgacatgaac gccgcggaac
    21601 ttagcgagca ggatgtctgg ggtaaatcca cccctgcggg tgacgaaata tggtaaagaa
    21661 aagtgaattt gaacggggag acattgtgct ggttggcttt gatccagcaa gcggccatga
    21721 acagcaaggt gctggtcgac ctgcgcttgt gctctccgtt caagccttta atcaactggg
    21781 aatgacgctg gtggccccca ttacgcaggg cggaaatttt gcccgttatg ccggatttag
    21841 cgttccttta cattgcgaag aaggcgatgt gcacggcgtg gtgctggtga atcaggtgcg
    21901 gatgatggat ctacgcgccc ggctggcaaa gcgtattggt ctggctgcgg atgaggtggt
    21961 ggaagaggcg ttattacgct tgcaggcggt ggtggaataa ggtgtgttta tttatcgcgg
    22021 gcataaaaaa acccttacta accgaagctc ggcgttcagg gttattgcgc tagaagaact
    22081 tatttattct ttgcgcgctc gaaggaagca acgatttcag ctttagcggc ttctgcgttt
    22141 tcccaacctt caactttcac ccacttgcct ttttcgaggt ctttgtagtg ctcgaagaag
    22201 tgagcgatct gcgctttcag cagctctggc agatcgttaa cgtctttaat gtgatcgtat
    22261 tctttgctca gcttgctgtg cggaaccgca accagtttcg catcttcacc ggcttcgtcg
    22321 gtcattttca gaacgccaac cggacggcaa cggatcacag aacccggctg cagcgggtac
    22381 ggagtcggga ccagtacgtc aaccgggtca ccgtccagag acagggtgtg gttgatgtaa
    22441 ccgtagttgc acggatagaa catcgcggtg gacatgaagc ggtcaacgaa cagtgcgccg
    22501 ctctctttgt cgatttcgta tttgatcgga tctgcgttag ccgggatctc aataacaacg
    22561 tagatgtctt ccggcagatc tttacccgca gggacgttga gtaagctcat gtctgtttcc
    22621 tttaaaaata tgtggcaaac aagtgccgag tattatagcc aactcgcgcc gaatgtcttt
    22681 gcttgttttc gtcttcattt tcctttttcg ccagttttaa gacggtatac agagcaggaa
    22741 aatccataac cgtagccgca tttttcatag tgagatgaaa gcgattacaa acttgtgatt
    22801 aacgttttat ttactttttt gaagtgtgat gtaacgcaat ccgttacata acgaattgtc
    22861 tatagttttt tcgcgaacat cttttcacca ataataatta ccccgacgag gacaacccta
    22921 tgtggaaacg cttacttgta gtctctgcag tctcggcagc catgtcgtct atggcgttgg
    22981 ccgctccatt aaccgtagga ttttcgcagg tcggatcgga atccggctgg cgcgctgcag
    23041 aaaccaatgt ggcgaaaagt gaggccgaaa aacgcggaat tacgttgaaa attgccgatg
    23101 gtcagcaaaa gcaggaaaac cagattaaag cggtacgttc cttcgttgca caaggggtgg
    23161 atgcgatctt tatcgctcca gtggttgcga cgggttggga accggtatta aaagaggcga
    23221 aagatgccga aatcccggtc ttcttgcttg accgttccat cgatgtgaaa gacaaatctc
    23281 tctatatgac caccgtcact gccgacaaca tcctcgaagg caagttgatt ggtgactggc
    23341 tggtaaaaga agtgaatggc aaaccatgca acgtggtgga actgcagggc accgttgggg
    23401 ccagcgtcgc cattgaccgt aagaaaggct ttgccgaagc cattaagaat gcgccaaata
    23461 tcaaaatcat ccgctcgcag tcaggtgact tcacccgcag taaaggcaaa gaagttatgg
    23521 agagctttat caaagcggaa aacaacggca aaaacatctg catggtttac gcccataacg
    23581 atgacatggt gattggtgca attcaggcaa ttaaagaagc gggcctgaaa ccgggcaaag
    23641 atatcctcac gggttccatt gacggcgtac cggatatcta taaagcgatg attgatggcg
    23701 aagcgaacgc cagcgttgaa ctgacgccga acatggcagg ccccgctttt gacgcgctgg
    23761 agaaatacaa aaaagacggc accatgcctg aaaagctgac cctgaccaaa tccacccttt
    23821 atctgcctga taccgcaaaa gaagagttag agaagaagaa aaatatgggg tattgagggt
    23881 tgctatgcct gatgccgatt cgtaggccgg ataaggcgct cgcgccgcat ccggcgatgg
    23941 tgcactgaag cctgatgcga cgcttaccgc gtcttatcat gcctactggg agcacgcttt
    24001 acaccggggg aaaccatgac gaccgaccaa caccaggaga tcctccgcac cgaaggatta
    24061 agtaaatttt tccccggcgt caaagcgtta gacaacgttg atttcagcct gcgccgtggc
    24121 gaaatcatgg cgctgcttgg tgaaaacggg gcgggaaaat caacgctaat caaagcatta
    24181 actggtgtat accacgccga tcgcggcacc atctggctgg aaggccaggc tatctcaccg
    24241 aaaaataccg cccatgcaca acaactcggt atcggcaccg tctatcagga agtcaacctg
    24301 ctacccaata tgtcggttgc tgataatcta tttataggcc gcgaacccaa acgtttcggc
    24361 cttctacgcc gtaaagagat ggaaaagcgc gccaccgaac tgatggcatc ttacggtttc
    24421 tccctcgacg tgcgcgaacc gcttaaccgt ttttcggtgg cgatgcagca aatcgtcgcc
    24481 atttgccggg ctatcgatct ctccgccaaa gtgctgatcc tcgatgaacc caccgccagc
    24541 ctcgacaccc aggaagtgga gctattattt ggcctgatgc gtcagttgcg cgatcgcggc
    24601 gtcagcctga tctttgtcac tcactttctc gatcaggtct atcaggtcag cgatcggatc
    24661 accgtcttac gcaacggcag tttcgtaggc tgtcgggaaa cgcgcgagct accgcagatc
    24721 gaactggtaa aaatgatgct ggggcgcgag ctggataccc acgcgctaca gcgtgccggg
    24781 cgaacattgt tgagcgacaa acccgttgcc gcgttcaaaa attacggcaa aaaaggaacg
    24841 atagcaccgt ttgatctcga agtgcgcccc ggcgagatcg tcggtctggc tggcttgctg
    24901 ggatccggac gtaccgaaac cgccgaagtg atcttcggta tcaaacctgc tgacagcggc
    24961 acggcgttga tcaaaggcaa accgcaaacc ctgcgatcgc cacatcaggc ttcagtactg
    25021 ggcatcggat tctgcccgga agacaggaaa accgatggca tcatcgctgc cgcctcggtg
    25081 cgggaaaata tcatcctcgc tctccaggcc cagcgcggct ggctacgacc catttcccgc
    25141 aaagagcagc aagagattgc cgaacgcttt atccgccagc ttggcattcg cacaccttca
    25201 actgaacaac cgattgaatt tctctctggc ggcaatcagc aaaaagtgtt gctttcacgt
    25261 tggctactga cacgaccgca atttctgatc ctcgacgagc caacccgcgg cattgatgtt
    25321 ggtgcccacg ccgagatcat ccgcctgatt gaaacgctgt gtgctgacgg tctggcgctg
    25381 ttggtgatct cctccgaact ggaagagctg gtgggctatg ccgatcgggt gattatcatg
    25441 cgcgatcgca aacaggtggc ggagatcccg ctggcagagc tttccgttcc ggcgatcatg
    25501 aacgccattg cggcgtaagg agaacagtgt gatgcctcaa tctctcccgg acactacgcc
    25561 gccgaaaagg cgctttcgct ggccaacggg aatgccgcag ctggcggcgc tattgctggt
    25621 gctgctggtc gatagcctgg tagccccgca tttctggcag gtggtgcttc aggatgggcg
    25681 tttgttcggt agccctatcg acattcttaa ccgtgcagct cccgttgcgt tactggcgat
    25741 tggtatgacg ctggtgatcg ccaccggtgg gattgatctc tccgtagggg cggtgatggc
    25801 tatcgccgga gccacaacgg ctgcgatgac ggtcgcggga ttcagcctgc cgattgtttt
    25861 gttaagcgcc ctgggcaccg gcatcctggc gggattgtgg aacggcatac tggtagcgat
    25921 cctcaaaatt cagccgtttg tcgccaccct gatcctgatg gtcgccgggc gcggcgtggc
    25981 gcaactgatc accgccgggc agatcgtcac gtttaactcg ccggatctct catggtttgg
    26041 cagcggatcg ctgttgttcc tgccaacgcc ggtcattatc gcggtgctga cgcttctcct
    26101 gttctggctg ttgacccgca aaacggcgct ggggatgttt atcgaagccg ttggtatcaa
    26161 cattcgggcg gcaaaaaatg ccggggtaaa cacgcggatc atcgtcatgc tcacctacgt
    26221 gttgagcggg ctgtgtgcgg cgattgcggg cattatcgtg gcggcggata ttcgcggtgc
    26281 cgatgccaat aacgccgggt tatggctgga gctggacgcc attcttgcgg tggtgattgg
    26341 cggcggatcg ctgatgggcg ggcgctttaa cctgctgctc tcagtcgtgg gggcgctgat
    26401 tattcagggg atgaacaccg gaattttact ttctggcttt ccgccggaga tgaaccaggt
    26461 cgtaaaagcg gtggtggtgc tttgtgtgct gattgttcag tcgcaacgct ttatcagtct
    26521 gattaaagga gtacgtagcc atgataaaac gtaacttgcc gctgatgatc accatcggcg
    26581 tctttgtgct gggctatctc tactgcctga cccagtttcc cggttttgct tccacaagag
    26641 tgatctgcaa tatcctgacc gataacgcct ttcttgggat aatcgccgtt ggcatgacct
    26701 ttgtgatcct ctctggcggt atcgatctct ccgtcggttc ggtgatcgcc tttactggcg
    26761 tgtttctggc aaaagtgatt ggcgatttcg gcctctcgcc gctgctggcg tttccgctag
    26821 tgctggtgat gggctgtgcc tttggcgcat ttatggggct tttgatcgac gccctgaaga
    26881 tcccggcatt tatcattacc ctggcaggaa tgttcttttt gcgcggcgtc agctatctcg
    26941 tttcagaaga gtcgatcccg ataaaccatc ccatttatga cacgctctca agccttgcgt
    27001 ggaaaatccc tggcggcggt cgcttaagtg cgatgggact gctgatgctg gcggtggtgg
    27061 ttatcggcat attcctcgcg catcgtaccc gttttggtaa tcaggtatac gccattggcg
    27121 gcaacgcaac gtcggcaaac ctgatgggga tttctacccg cagcaccact attcgcattt
    27181 atatgctctc caccggactg gcaacgctgg cggggattgt cttctcgatt tatacccagg
    27241 ccggatatgc gctggcgggc gtaggtgtgg aactggatgc tatcgcctca gtggtaattg
    27301 gcggtacgct tttgagcggt ggcgttggaa cggtattagg gacgcttttt ggcgtggcga
    27361 ttcaggggct aattcagact tatatcaact ttgacggtac gctgagttcc tggtggacga
    27421 aaatcgccat cggcattttg ctgttcattt ttattgcctt acagcgtgga ttaacggtgc
    27481 tgtgggagaa tcgtcagagt tcgccagtga caagagtcaa tattgcgcag cgataaaaac
    27541 gcctctccgt gtggagaggc gcaggagatt acgcgtccgg gaattcacgg ataaagcgtt
    27601 cgacatcttc aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt
    27661 ccgggatgat atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg
    27721 ccaggaatgc catcgggttg cactcataca gcaaacgcag tttgccgtcc gggtggctgg
    27781 cggtgcttgg gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcgacca
    27841 gtgaaccgat ataacgtgag gtatacgggc ggttggtgga tttatcttct tcctggcaga
    27901 atttaatgta cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt
    27961 aggttttgcc tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg
    28021 aaggatcgta agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg
    28081 agccgtatac cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt
    28141 ccgttaccgg cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag
    28201 agacgttaac atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg
    28261 cgtgttcaca gccttcaaag acgacaatct catcttcttc ttcagaggca atgcccgcaa
    28321 cgatatcgcg tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagtttct
    28381 gctgaacctc gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg
    28441 ctttgttgat atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag
    28501 tgagctcacc ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg
    28561 ttttcataaa actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa
    28621 ataatctcaa tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg
    28681 cgtttctcac ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac
    28741 gtatgcgcat tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg
    28801 cgcgccagtt aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca
    28861 ccttacttga gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgatc
    28921 cgcagccgga tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag
    28981 cggtactgga aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc
    29041 tgcgcgaccg ctgggtgctg gccgttgccg gtacacacgg caaaactacc accgcgggaa
    29101 tggcgacctg gattctggaa cagtgcggtt acaaaccggg atttgtgatc ggcggtgtgc
    29161 cggggaactt tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg
    29221 atgagtatga ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta
    29281 cgctgatcct caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga
    29341 tccagaaaca gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc
    29401 cggaaaatga catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc
    29461 tggtgggtga gcagggtcac tggcaggcga aaaagctgac cactgacgct tctgaatggg
    29521 aagtcttgct ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggtg ggcgaacata
    29581 atatgcacaa tggcctgatg gcgattgcgg cggctcgcca tgttggtgta gcgcctgcag
    29641 atgccgctaa cgcgctgggt tcgtttatta acgctcgtcg ccgtctggag ttgcgtggtg
    29701 aagcgaatgg cgtgacggta tatgacgatt ttgcccatca cccgacggca attctggcaa
    29761 cgcttgcggc gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggagc
    29821 cgcgctcgaa taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc
    29881 gtgccgatga agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg
    29941 cagaagcctg cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg
    30001 tggtgaaaac cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg
    30061 ggatccatca gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg
    30121 gcctcagcct gatatagtgt gcgtaggccg gataaggcgt tcacgccgca tccggcattt
    30181 gagcatagtg cctgatgcga cgcttgatgc gtcttatcag gcctacaggt gcaccgcatc
    30241 cggcaaacca tactcacatc aacaacgaaa attacccttc attctccgcc agttcgcgca
    30301 gatactggaa aatctggcgt gcggatttcg gcggcttatt cccttctttc tctttcttcg
    30361 cgttgcggat cagagtacgc agctgctgac gatcggcatc gtgccacaga tttaatacct
    30421 cagcgatggc gtcatcacct tgatcgatca gacgatcgcg caggttttcc agtttatgga
    30481 acagcaccac ctgctggttg tgacggtttt tcagcttatc cagcgcctga cggatcggtt
    30541 ctacatcgcg ctggcgcagc atcttaccga tgagctgcaa ctggcggcgg cgaccttcca
    30601 tcttaatacg ctgggcgagc tcaatagccg cgcgcagatc cgcatctaac gggatcttat
    30661 ccagcgcgtt tttccccaga tcaacaattt ccgcgccaag gcgttttagc tcctcggcat
    30721 cacgtttaat ttcactttta ctgacccaga taatttcatc gtcttcgtct tcgatgtcat
    30781 caccgggaac gtcgtcgagc cagtcttcgg gctgcttagt catctcaggc tccttaaaaa
    30841 aagaggctaa tgttaccagt taagatgcgc actgaaaaac ggttctctgt tagacttcag
    30901 agaaactctc tacattatgg cacttgcaat gaaagtaatc tctcaagttg aagcgcagcg
    30961 caagattctg gaagaagcag tttccactgc gctggagttg gcctcaggca aatcggacgg
    31021 tgcggaagtt gccgtcagca agaccaccgg cattagcgta agcacgcgtt atggtgaagt
    31081 ggagaatgtt gaattcaata gcgatggcgc gctggggatc actgtttatc accagaaccg
    31141 caaaggtagc gcatcatcca ccgatttaag cccgcaggcc attgcccgta ctgtacaggc
    31201 ggcgctggat attgcccgtt atacctcgcc agatccctgt gccggcgtgg cagacaaaga
    31261 gctgctggcc tttgacgcac cagatctcga cttgttccac cctgcggaag tttccccgga
    31321 tgaagccatt gaactggcgg cccgcgcaga acaggcggca ttgcaggcgg acaaacgcat
    31381 taccaatacc gaaggtggca gctttaacag ccactacggt gtcaaagttt ttggcaacag
    31441 ccacggcatg ttgcagggtt actgctcaac gcgtcattcg ctctccagct gtgtaattgc
    31501 cgaagaaaat ggcgatatgg agcgtgatta cgcctacacc attggccgcg cgatgagcga
    31561 tctgcaaacg ccagagtggg tcggggccga ctgtgctcgc cgtactttat cgcgcctgtc
    31621 accgcgtaaa ctctccacca tgaaagcgcc agtcattttt gccaatgaag tggcaaccgg
    31681 gctttttggc catctggtgg gggcgatagc gggtggctcg gtttaccgta aatctacctt
    31741 tctgctggat tcgctgggta aacaaattct gccggactgg ctgaccattg aagagcatcc
    31801 gcatctgctg aaagggctgg cgtcgacgcc attcgacagc gaaggtgtgc gcaccgagcg
    31861 tcgcgatatt attaaagatg gcatcctgac tcagtggctg ctgaccagct actcggcgcg
    31921 gaaactgggg ctgaaaagca ccggacatgc gggcggtatt cacaactggc ggattgccgg
    31981 acaagggcta agcttcgaac aaatgctcaa agagatgggc accgggctgg tggtgacgga
    32041 gctgatgggc cagggcgtga gtgccattac cggtgattat tcccgtggtg cagcgggctt
    32101 ctgggtagag aatggcgaaa ttcagtatcc cgttagtgaa atcaccatcg caggtaattt
    32161 aaaagatatg tggcgcaata ttgtcaccgt cggtaacgat attgaaacac gcagtaatat
    32221 acagtgtggt tctgtgctgt tgccggagat gaaaatcgcc gggcagtaaa aaactggcgc
    32281 gacctgccgc gctataaact aaaaaattac acaaaatcat tcgcactgca tcgagacgga
    32341 aagtgaatga gggccgggga gcgtactcgc agtacgtgac ccgggcgaat gagcgtagcc
    32401 gtcgatgagg cagtgtgaag gatgaagtgt aaataaaaaa ggaagtgagc aatgcgtaaa
    32461 agcctgttag ctattcttgc agtctcctcg ttggtattca gttctgcgtc gtttgccgct
    32521 gatcttgaag a
//
LOCUS       DAZFWL020000043        32197 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFWL020000043 DAZFWL020000000
VERSION     DAZFWL020000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.043"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            90..389
                     /locus_tag="ACHKNP_RS10705"
                     /old_locus_tag="pgaptmp_002141"
     CDS             90..389
                     /locus_tag="ACHKNP_RS10705"
                     /old_locus_tag="pgaptmp_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704304.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(436..1323)
                     /gene="rarD"
                     /locus_tag="ACHKNP_RS10710"
                     /old_locus_tag="pgaptmp_002142"
     CDS             complement(436..1323)
                     /gene="rarD"
                     /locus_tag="ACHKNP_RS10710"
                     /old_locus_tag="pgaptmp_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HGU4704305.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1375..1842)
                     /gene="yigI"
                     /locus_tag="ACHKNP_RS10715"
                     /old_locus_tag="pgaptmp_002143"
     CDS             complement(1375..1842)
                     /gene="yigI"
                     /locus_tag="ACHKNP_RS10715"
                     /old_locus_tag="pgaptmp_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HGU4704306.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2007..2876
                     /gene="pldA"
                     /locus_tag="ACHKNP_RS10720"
                     /old_locus_tag="pgaptmp_002144"
     CDS             2007..2876
                     /gene="pldA"
                     /locus_tag="ACHKNP_RS10720"
                     /old_locus_tag="pgaptmp_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HGU4704307.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3009..4838
                     /gene="recQ"
                     /locus_tag="ACHKNP_RS10725"
                     /old_locus_tag="pgaptmp_002145"
     CDS             3009..4838
                     /gene="recQ"
                     /locus_tag="ACHKNP_RS10725"
                     /old_locus_tag="pgaptmp_002145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HGU4704308.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4902..5522
                     /gene="rhtC"
                     /locus_tag="ACHKNP_RS10730"
                     /old_locus_tag="pgaptmp_002146"
     CDS             4902..5522
                     /gene="rhtC"
                     /locus_tag="ACHKNP_RS10730"
                     /old_locus_tag="pgaptmp_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HGU4704309.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5584..6204)
                     /gene="rhtB"
                     /locus_tag="ACHKNP_RS10735"
                     /old_locus_tag="pgaptmp_002147"
     CDS             complement(5584..6204)
                     /gene="rhtB"
                     /locus_tag="ACHKNP_RS10735"
                     /old_locus_tag="pgaptmp_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HGU4704310.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6315..7337
                     /gene="pldB"
                     /locus_tag="ACHKNP_RS10740"
                     /old_locus_tag="pgaptmp_002148"
     CDS             6315..7337
                     /gene="pldB"
                     /locus_tag="ACHKNP_RS10740"
                     /old_locus_tag="pgaptmp_002148"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HGU4704311.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7345..8145
                     /gene="yigL"
                     /locus_tag="ACHKNP_RS10745"
                     /old_locus_tag="pgaptmp_002149"
     CDS             7345..8145
                     /gene="yigL"
                     /locus_tag="ACHKNP_RS10745"
                     /old_locus_tag="pgaptmp_002149"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HGU4704312.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8221..9120
                     /gene="bioP"
                     /locus_tag="ACHKNP_RS10750"
                     /old_locus_tag="pgaptmp_002150"
     CDS             8221..9120
                     /gene="bioP"
                     /locus_tag="ACHKNP_RS10750"
                     /old_locus_tag="pgaptmp_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HGU4704313.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9008..9961)
                     /gene="metR"
                     /locus_tag="ACHKNP_RS10755"
                     /old_locus_tag="pgaptmp_002151"
     CDS             complement(9008..9961)
                     /gene="metR"
                     /locus_tag="ACHKNP_RS10755"
                     /old_locus_tag="pgaptmp_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HGU4704314.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10079..12340
                     /gene="metE"
                     /locus_tag="ACHKNP_RS10760"
                     /old_locus_tag="pgaptmp_002152"
     CDS             10079..12340
                     /gene="metE"
                     /locus_tag="ACHKNP_RS10760"
                     /old_locus_tag="pgaptmp_002152"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGU4704315.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(12379..13194)
                     /gene="ysgA"
                     /locus_tag="ACHKNP_RS10765"
                     /old_locus_tag="pgaptmp_002153"
     CDS             complement(12379..13194)
                     /gene="ysgA"
                     /locus_tag="ACHKNP_RS10765"
                     /old_locus_tag="pgaptmp_002153"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HGU4704316.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13456..14217
                     /gene="udp"
                     /locus_tag="ACHKNP_RS10770"
                     /old_locus_tag="pgaptmp_002154"
     CDS             13456..14217
                     /gene="udp"
                     /locus_tag="ACHKNP_RS10770"
                     /old_locus_tag="pgaptmp_002154"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HGU4704317.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14358..15785
                     /gene="rmuC"
                     /locus_tag="ACHKNP_RS10775"
                     /old_locus_tag="pgaptmp_002155"
     CDS             14358..15785
                     /gene="rmuC"
                     /locus_tag="ACHKNP_RS10775"
                     /old_locus_tag="pgaptmp_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGU4704318.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFSLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15880..16635
                     /gene="ubiE"
                     /locus_tag="ACHKNP_RS10780"
                     /old_locus_tag="pgaptmp_002156"
     CDS             15880..16635
                     /gene="ubiE"
                     /locus_tag="ACHKNP_RS10780"
                     /old_locus_tag="pgaptmp_002156"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HGU4704319.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16649..17254
                     /gene="ubiJ"
                     /locus_tag="ACHKNP_RS10785"
                     /old_locus_tag="pgaptmp_002157"
     CDS             16649..17254
                     /gene="ubiJ"
                     /locus_tag="ACHKNP_RS10785"
                     /old_locus_tag="pgaptmp_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HGU4704320.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17251..18891
                     /gene="ubiB"
                     /locus_tag="ACHKNP_RS10790"
                     /old_locus_tag="pgaptmp_002158"
     CDS             17251..18891
                     /gene="ubiB"
                     /locus_tag="ACHKNP_RS10790"
                     /old_locus_tag="pgaptmp_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HGU4704321.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18970..19239
                     /gene="tatA"
                     /locus_tag="ACHKNP_RS10795"
                     /old_locus_tag="pgaptmp_002159"
     CDS             18970..19239
                     /gene="tatA"
                     /locus_tag="ACHKNP_RS10795"
                     /old_locus_tag="pgaptmp_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HGU4704322.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19243..19758
                     /gene="tatB"
                     /locus_tag="ACHKNP_RS10800"
                     /old_locus_tag="pgaptmp_002160"
     CDS             19243..19758
                     /gene="tatB"
                     /locus_tag="ACHKNP_RS10800"
                     /old_locus_tag="pgaptmp_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HGU4704323.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19761..20537
                     /gene="tatC"
                     /locus_tag="ACHKNP_RS10805"
                     /old_locus_tag="pgaptmp_002161"
     CDS             19761..20537
                     /gene="tatC"
                     /locus_tag="ACHKNP_RS10805"
                     /old_locus_tag="pgaptmp_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HGU4704324.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20579..21361
                     /gene="tatD"
                     /locus_tag="ACHKNP_RS10810"
                     /old_locus_tag="pgaptmp_002162"
     CDS             20579..21361
                     /gene="tatD"
                     /locus_tag="ACHKNP_RS10810"
                     /old_locus_tag="pgaptmp_002162"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HGU4704325.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21358..21846)
                     /gene="rfaH"
                     /locus_tag="ACHKNP_RS10815"
                     /old_locus_tag="pgaptmp_002163"
     CDS             complement(21358..21846)
                     /gene="rfaH"
                     /locus_tag="ACHKNP_RS10815"
                     /old_locus_tag="pgaptmp_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HGU4704326.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22013..23506
                     /gene="ubiD"
                     /locus_tag="ACHKNP_RS10820"
                     /old_locus_tag="pgaptmp_002164"
     CDS             22013..23506
                     /gene="ubiD"
                     /locus_tag="ACHKNP_RS10820"
                     /old_locus_tag="pgaptmp_002164"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HGU4704327.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23552..24253
                     /gene="fre"
                     /locus_tag="ACHKNP_RS10825"
                     /old_locus_tag="pgaptmp_002165"
     CDS             23552..24253
                     /gene="fre"
                     /locus_tag="ACHKNP_RS10825"
                     /old_locus_tag="pgaptmp_002165"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HGU4704328.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24445..25608)
                     /gene="fadA"
                     /locus_tag="ACHKNP_RS10830"
                     /old_locus_tag="pgaptmp_002166"
     CDS             complement(24445..25608)
                     /gene="fadA"
                     /locus_tag="ACHKNP_RS10830"
                     /old_locus_tag="pgaptmp_002166"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HGU4704329.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25618..27807)
                     /gene="fadB"
                     /locus_tag="ACHKNP_RS10835"
                     /old_locus_tag="pgaptmp_002167"
     CDS             complement(25618..27807)
                     /gene="fadB"
                     /locus_tag="ACHKNP_RS10835"
                     /old_locus_tag="pgaptmp_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HGU4704330.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALKRPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27997..29328
                     /gene="pepQ"
                     /locus_tag="ACHKNP_RS10840"
                     /old_locus_tag="pgaptmp_002168"
     CDS             27997..29328
                     /gene="pepQ"
                     /locus_tag="ACHKNP_RS10840"
                     /old_locus_tag="pgaptmp_002168"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HGU4704331.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29328..29942
                     /gene="yigZ"
                     /locus_tag="ACHKNP_RS10845"
                     /old_locus_tag="pgaptmp_002169"
     CDS             29328..29942
                     /gene="yigZ"
                     /locus_tag="ACHKNP_RS10845"
                     /old_locus_tag="pgaptmp_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HGU4704332.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29981..31432
                     /gene="trkH"
                     /locus_tag="ACHKNP_RS10850"
                     /old_locus_tag="pgaptmp_002170"
     CDS             29981..31432
                     /gene="trkH"
                     /locus_tag="ACHKNP_RS10850"
                     /old_locus_tag="pgaptmp_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HGU4704333.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFLVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31444..31989
                     /gene="hemG"
                     /locus_tag="ACHKNP_RS10855"
                     /old_locus_tag="pgaptmp_002171"
     CDS             31444..31989
                     /gene="hemG"
                     /locus_tag="ACHKNP_RS10855"
                     /old_locus_tag="pgaptmp_002171"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HGU4704334.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7476 a         8170 c         8803 g         7748 t
ORIGIN      
        1 aagtttttat tgatggggta tattttaatt aaatataatt gaaatgtatt tttattctat
       61 aaacaatatc ttgggtgaat ggattctata tgggaaatgg taacttttgg cagttgttct
      121 tgggtaaaga accttacagc aaaaaatctg attttgttaa ggcatggtta ttgtggtggg
      181 ctattaccat ccttgttcta tttattttaa gcctgactac gacattaaat ttaagaagta
      241 taggtatagg gttaagtttt gggttttgtc aaatccttgg tagatatacg atgtctctag
      301 gatggagtaa gtactgggga gttttggggg tatttcctgt aacaaatatt gttctttttc
      361 ttatcttaat ttttttcaaa aaaagataaa taagtcagtg ggtaggctct ttaaacggcc
      421 tacccatgtt aattcttaac tttttctacg ctgcgtataa atcgcatcca tcacaaaaat
      481 tgccagcgcc acccaaataa aggcgaaagt caccatctta tcggcacccg gtttttcacc
      541 ataaaacgtc acagccagca ggaacatcag cgtcgggcca atgtactgga aaaagcctaa
      601 cgttgagaga cgcaagcgcg tggcagcggc ggtaaaacac aacagcggta cggtagtgac
      661 aataccggcg gcgatcagca gtaaattcag cgacatcggg ttttgcccca tatggctggt
      721 tgagctgtcg gcaatagcaa acaggtaaat tgccgccacg ggcagcagcc acatggtttc
      781 gattaacatg ccggtttgcg cttcaacggc aatcttcttg cgtaccagac cgtagaaggc
      841 aaaactaaat gccagtccca gcgcgataat aggtagcgaa ccaaaagtcc acagctggac
      901 taacacgcca catatcgcca gaatcaccgc cagccattgc atccggcgga atcgctcgcc
      961 gaggaaaatc atccccagca caatgttcac cagcgggtta ataaagtaac caaggctcgc
     1021 ttccagcata tggtgattgt tcaccgccca gataaacagt agccagttgc caccaatcag
     1081 cacggcagag actgccagca taaaaatttt ctgtggcgtc tgaatcagcg tttttaaata
     1141 ggaccactgg cggcaaatgc tcatcagcac caccataaag aaaaacgacc agatcacgcg
     1201 atgcgtcagg atttcatcgg caggcacgta gtaaatcaac ttgaagtacg ctggcgctat
     1261 accccaaata aaataagcgg caagagcgag taatacgccc tgccgcgttt gttttgcatc
     1321 catcgggaat actcattttt aattggtaac agcagtttac ctgcttttat gtcttcaacc
     1381 taccatatag gtggcggtgg cactggcaat ataaagctgt tcttcattgt gtaattcaac
     1441 gcgggcgacg gcgactttat tgcctgcacg caacaggcta ctggtagcag taaaacgctc
     1501 gcccctgcct gggcgcagat aatcaacgcg aagatcaatg gtccccatcc gcgatagccg
     1561 ctggcgtagt tcatcttcac tgatggtttc gtggcgggtt aaggtacttc ccacgcacac
     1621 cagacctgcg gcgacatcca gcgctgacgc aataaccccg ccgtgcaaaa tgctttgcgc
     1681 ccagttaccc accatcattg gctgattttt aaaggccagc tgtgcgaact ctttttcgta
     1741 acgctccagc tccatcccta acgcgcggtt aaatggcatg tgataaacaa acatctcacc
     1801 cactaatttc agggcttgtt cagcggtcag tacggcagac atatcatcct tacacttcat
     1861 tggttaatga aatgttgatt ttatgcttct ttgttgttgg tttctacttt aggaagggat
     1921 aactaacggc tatggagtta agtatgtaaa atagcccgca gaaaaatatt caccttatca
     1981 ataattcgtt acggagaaca cgaccgatgc ggactctgca gggctggttg ttgccggtgt
     2041 ttatgttgcc tatggcagta tatgcacaag aggcaacggt gaaagaggtg catgacgcgc
     2101 cagcggtgcg tggcagtatt atcgccaata tgctgcagga gcatgacaat ccgttcacgc
     2161 tctatcctta tgacaccaac tacctcattt acacccaaac cagcgatctg aataaagaag
     2221 cgattgccag ttacgactgg gcggaaaatg cgcgtaagga tgaagtaaag tttcagttga
     2281 gcctggcatt tccgctgtgg cgtgggattt taggcccgaa ctcggtgttg ggtgcgtctt
     2341 atacgcaaaa atcctggtgg caactgtcca atagcgaaga gtcttcaccg tttcgtgaaa
     2401 ccaactacga accgcaattg ttcctcggtt ttgccaccga ttaccgtttt gcaggttgga
     2461 cgctgcgcga tgtggagatg gggtataacc acgactctaa cgggcgttcc gacccgacct
     2521 cccgcagctg gaaccgcctt tatactcgcc tgatggcaga aaacggtaac tggctggtag
     2581 aagtgaagcc gtggtatgtg gtgggtaata ctgacgataa cccggatatc accaaatata
     2641 tgggttacta ccagcttaaa atcggctatc acctcggtga tgcggtgctc agtgcgaaag
     2701 gacagtacaa ctggaacacc ggctacggcg gcgcggagtt aggcttaagt tacccgatca
     2761 ccaaacatgt gcgcctttat actcaggttt acagcggcta tggcgaatcg ctcatcgact
     2821 ataacttcaa ccagacccgt gtcggtgtgg gggttatgct aaacgatttg ttttgatgaa
     2881 cggttgagtg gttggcaaat ctggaaccca gcatccagga ttaccctctc agagactaaa
     2941 agcattgcag tttctcgcgc aggcgctgaa aatagcgcct gtttttattt caggcaatcg
     3001 gggtgaatgt ggcgcaggcg gaagtgttga atctggagtc cggagctaaa caggttttac
     3061 aagaaacctt tggctaccaa cagtttcgcc ccggccagga agaaattatc gacactgtgc
     3121 tttccggtcg cgattgcctg gtcgtcatgc ccaccggtgg cggaaaatcc ctttgctatc
     3181 aaattcctgc cttattgcta aacggcctta ccgtggttgt ttcaccgctg atttcgttga
     3241 tgaaagatca ggtggatcaa ctgcaagcca acggcgtggc ggcggcgtgc cttaactcga
     3301 cgcaaacccg cgaacagcaa cttgaagtga tgacaggctg ccgcaccggg caaattcgtc
     3361 tgctttatat cgccccggaa cgcctgatgc tggataactt tcttgagcat ctggcgcact
     3421 ggaatccggt gttattagcc gttgatgaag cgcactgtat ctcccaatgg ggccacgatt
     3481 tccgcccgga atatgccgcg ctcggtcagt tgcgccagcg gttcccgacg ctgccgttta
     3541 tggcgctgac cgccacagcc gacgacacca cgcgccagga tatcgtgcgc ctgctggggc
     3601 tgaacgatcc gctgattcaa atcagcagtt ttgaccgtcc gaatattcgc tacatgctga
     3661 tggagaagtt caaaccgctc gatcagttga tgcgctacgt gcaggaacag cgcggtaagt
     3721 ccggcattat ctactgcaac agccgggcga aagtagaaga caccgctgcg cgcctgcaaa
     3781 gcaagggtat tagcgcggcg gcctatcatg ccgggctgga aaataatgtc cgcgccgacg
     3841 tgcaggagaa attccagcgc gatgacctgc aaattgtggt ggcgacggtg gcgttcggca
     3901 tgggcatcaa taaaccaaac gttcgcttcg tggtccactt tgatattccg cgcaatatcg
     3961 aatcctatta tcaggaaacc ggtcgcgccg ggcgtgatgg cctgcccgcg gaagcgatgc
     4021 tgttttacga tccggccgat atggcgtggc tgcgccgttg tctggaagag aagccgcagg
     4081 ggcagttgca ggatatcgaa cgtcataaac tcaatgcgat gggcgctttt gccgaagcgc
     4141 aaacttgccg tcgtctggta ctgctgaact attttggcga agggcgtcag gagccgtgcg
     4201 ggaactgcga tatctgcctc gatccgccga aacagtacga cggttcaacc gatgctcaga
     4261 ttgccctttc caccattggt cgtgtgaatc agcgttttgg tatgggctat gtggtggaag
     4321 tgattcgcgg cgctaataac cagcgtatcc gcgactatgg tcatgacaaa ctaaaagtct
     4381 acggtatggg ccgtgataaa agtcatgaac attgggtgag cgtgatccgc cagctgattc
     4441 acctcggtct ggtgacgcaa aatattgccc agcattctgc cctacaactg acagaggctg
     4501 cgcgtccggt gctgcgcggc gaatcctctt tgcaacttgc cgtgccgcgt atcgtggcgc
     4561 tcaaaccgaa agcgatgcag aaatcgttcg gcggcaacta tgatcgcaaa ctgttcgcca
     4621 aattacgcaa actgcgtaaa tcgatagccg atgaaagtaa tgtcccgccg tacgtggtgt
     4681 ttaacgacgc aaccttgatt gagatggctg aacagatgcc gatcaccgcc agcgaaatgc
     4741 tcagcgttaa cggcgttggg atgcgcaagc tggaacgctt tggcaaaccg tttatggcgc
     4801 tgattcgtgc gcatgttgat ggcgatgacg aagagtagtc agcagcataa aaaagtgcca
     4861 gtatgaagac tccgtaaacg tttcccccgc gagtcaaatg tatgttgatg ttatttctca
     4921 ccgtcgccat ggtgcacatt gtggcgctta tgagccccgg tcccgatttc ttttttgtct
     4981 ctcagaccgc tgtcagtcgt tcccgtaaag aagcgatgat gggcgtgctg ggcattacct
     5041 gcggcgtaat ggtttgggct gggattgcgc tgcttggcct gcatttgatt atcgaaaaaa
     5101 tggcctggct gcatacgctg attatggtgg gcggtggcct gtatctctgc tggatgggtt
     5161 accagatgct acgcggtgca ctgaaaaaag aggcggtttc tgcacctgcg ccacaggtcg
     5221 agctggcgaa aagtgggcgc agtttcctga aaggtttact gaccaatctc gctaatccga
     5281 aagcgattat ctactttggc tcggtgttct cattgtttgt cggtgataac gttggcacta
     5341 ccgcgcgctg gggcattttt gcgctgatca ttgtcgaaac gctggcgtgg tttaccgtcg
     5401 ttgccagcct gtttgccctg ccgcaaatgc gccgtggtta tcaacgtctg gcgaagtgga
     5461 ttgatggttt tgccggggcg ttatttgccg gatttggcat tcatttgatt atttcgcggt
     5521 gatgccagac gcgtcttcag agtaagtcgg ataaggcgtt tacgccgcat ccgacattat
     5581 ttttcacgca tgcctcgccg atgctaacag cgctcccacc agcataaaca acgagccgaa
     5641 aatcttattc agcgccttca tctgctttgg tcctttaatc catagagcaa tccgttgagc
     5701 aagggtggcg taaccgatca tcacaataat atcgaccaca atagtggtga cgccgagcac
     5761 gatatactgc atcagttgcg gctgttgcgg catgatgaat tgcggaaata gcgccgccag
     5821 aaacacaata cttttgggat tggtgagatt cacaaaaact gcgcgctgga acaaatgtcg
     5881 acgcgattga gtagaggcca gcgatttaag gtcaattgca ccagcggcgc gccactgctg
     5941 gattcccagc caaatcaagt aagccgcgcc tgcccacttc aacacttcaa acgcaatcac
     6001 tgagcgggaa aatagcgtcc ccaaccccac gccaaccagc acaatatgaa tcgccagtcc
     6061 ggtctgaagc ccagcaatag acgccaccgc gccgcgataa ccgtggttga gcgaggtggt
     6121 catagtgttg attgcaccag agcctggcga caggcttaaa atgatcgatg tcagcaggta
     6181 ggcaaaccac cattctaagg tcatgatgaa ctcccggtgt gtctattttt gtgccacaat
     6241 acgctactgt cgcagcgttg tgtcaggcac gctaaaaaaa acgattttac gtggtttaag
     6301 aggcagatta cccgatgttt cagcagcaaa aagactggga aacaagagaa aacgcgtttg
     6361 ctgcttttac catgggaccg ctgactgatt tctggcgtca gcgtgatgaa gcagagttta
     6421 ctggtgtgga tgacattccg gtgcgctttg tccgttttcg cgcacagcac catgaccggg
     6481 tggtagtcat ctgcccgggg cgtattgaga gctacgtaaa atatgcggaa ctggcctatg
     6541 acctgttcca tttggggttt gatgtcttaa tcatcgacca tcgcgggcag ggacgttccg
     6601 gtcgcctgtt agccgatccg catctcgggc atgttaatcg ctttaatgat tatgttgatg
     6661 atctggcggc attctggcag caggaggttc agcccggtcc gtggcgtaaa cgctatatac
     6721 tggcacattc gatgggcggt gcgatctcca cattatttct gcaacgccat ccaggtgtat
     6781 gtgacgccat tgcgctaact gcgccaatgt ttgggatcgt gattcgtatg ccgtcattta
     6841 tggcacggca gatcctcaac tgggccgaag cgcatccacg tttccgtgat ggctatgcaa
     6901 taggcaccgg gcgctggcgc gcgttgccgt ttgctatcaa cgtactgacc cacagcagac
     6961 agcgatatcg acgtaactta cgcttctatg ctgatgaccc aacgattcgc gtcggtgggc
     7021 cgacctacca ttgggtacgc gaaagtattc tggctggcga acaggtgtta gccggtgcgg
     7081 gtgatgacgc cacgccaacg cttctcttac aagctgaaga ggaacgcgtg gtggataacc
     7141 gcatgcatga ccgtttttgt gaactccgca ccgccgcggg ccatcctgtc gaaggaggac
     7201 ggccgttggt aattaaaggt gcttaccatg agatcctttt tgaaaaggac gcaatgcgct
     7261 cagtcgcgct ccacgccatc gttgattttt tcaacaggca taactcaccc agcggaaacc
     7321 gctctacaga ggtttaaatt tcttatgtac caggttgttg cgtctgattt agatggcacg
     7381 ttactttctc ccgaccatac gttatcccct tacgccaaag aaactctgaa gctgctcacc
     7441 gcgcgcggca tcaactttgt gtttgcgacc ggtcgtcacc acgttgatgt ggggcaaatt
     7501 cgcgataatc tggagattaa gtcttacatg attacctcca atggtgcgcg cgttcacgat
     7561 ctggatggta atctgatttt tgctcataac ctggatcgcg acattgccag cgatctgttt
     7621 ggcgtagtca acgacaatcc ggacatcatt actaacgttt atcgcgacga cgaatggttt
     7681 atgaatcgcc atcgcccgga agagatgcgc ttttttaaag aagcggtgtt caaatatgcg
     7741 ctgtatgagc ctggattact ggagccggaa ggcgtcagca aagtgttttt cacctgcgat
     7801 tcccatgaac aactgctgcc gctggagcag gcgattaacg ctcgctgggg cgatcgcgtc
     7861 aacgtcagtt tctctacctt aacctgtctg gaagtgatgg cgggcggcgt gtcgaaaggc
     7921 catgcgctgg aagcagtggc gaagaaactg ggctacagcc tgaaggattg tattgcgttt
     7981 ggtgacggga tgaacgacgc cgaaatgctg tcgatggcgg ggaaaggctg cattatgggc
     8041 agtgcgcacc agcgtctgaa agaccttcat cccgagctgg aagtgattgg tactaatgcc
     8101 gacgacgcgg tgccgcatta tctgcgtaaa ctctatttat cgtaatcgtt ctttatttgg
     8161 tcagttgtca acctgatact tcgctacaat ggatactcgt taatcaaaga gttttccatt
     8221 gtggcgctac ttatcatcac cacgattctg tgggccttct cctttagctt ttatggcgag
     8281 taccttgcgg ggcacgtcga tagctatttt gcggtgctgg tgcgcgttgg cctggcggca
     8341 ctcgtttttc tgccgtttct gcgtacccgt ggcaatagcc tgaaaacggt cggcctgtat
     8401 atgttggtgg gcgcgatgca gctcggcgtg atgtatatgc tgagtttccg cgcgtatctc
     8461 tacctgacgg tctccgaact gctgctgttc accgtactga cgccgctcta catcacgctg
     8521 atttatgaca tcatgagtaa gcgccgtctg cgctggggct atgcctttag cgccttgttg
     8581 gcggtgattg gtgccgggat tattcgctat gatcaggtca ccgaccattt ctggactggc
     8641 ttgctgctgg tgcaactctc caatatcact tttgccatcg gcatggtggg ttacaaacgc
     8701 ctgatggaaa ctcgcccgat gccacagcat aacgcctttg cgtggttcta tcttggcgcg
     8761 tttctggtgg cggtaattgc atggttcttg ctgggaaatg cgcagaaaat gccgcaaacc
     8821 acgttgcaat ggggcattct ggtgtttctt ggcgtggtgg cttccgggat tggctacttt
     8881 atgtggaact acggcgcgac gcaggtggac gccggaacgc tgggcattat gaataatatg
     8941 cacgttccgg cagggctgct ggtaaacctg gctatctggc accaacagcc gcactggcca
     9001 acgttcatta caggcgcgct ggtgatcctg gcctcactgt gggtgcatcg taagtgggtc
     9061 gctccgcgct cttcacaaac ggcagatgat cgcaggcgtg attgcgcgct gagcgaataa
     9121 atgcttctgt aatcggctga cgctgctcgc catcgcgcac ggcggcgtac agtcggctcc
     9181 acaagccttc gcccagggtt ttggtcacca ccagaccctg gcgctcaaaa ctctctacta
     9241 cccaatgcgg tagcgcggca atacccatcc gcgcggcaac catctgaatc aacaataagg
     9301 tgttatcgac gcttttcagt gacgggctga cgcctgccgg ctgaagaaaa tgccgccaga
     9361 catccagtcg actacgctgc accggataaa tcaacagcgt ctcgctggcg agatcttccg
     9421 gtgtaattcg cgttttcgcc gccagtggat ggtcaggtgc taacaccaga cgcacttcat
     9481 agtcgaacat cggcgaataa tgcaggccac tgcgcggcag gatatcggag gtcatcacca
     9541 gatccagctc gccctgttgc aacgcgggct gcgggtcaaa cgtcacgccc gatttaaaat
     9601 ccatctctac ctgcggccag ttcttatgga aattttctaa cgcgggcgtc agccactgaa
     9661 tacagctatg gcactcaatg gcaatgcgca gacgcgtctg ctgcggttca ttgcaggctt
     9721 gcagtgcctg gctaatttgc ggcagcacct ggtttgccag ttgcaacagg atttctccct
     9781 gcggtgtaaa gcgtagcggc tggctcttac gcacaaatag ccggaagcca aggcgttgtt
     9841 ccagatcgct aaactggtga gacagggcgg attgcgtctg atgcaacgtc gccgcagcgg
     9901 ctgcgagaga gccgcagttc cgcaacgctt gtagcgtttt caggtgtttt acttcgatca
     9961 tgaaagtcct tcacttcggc atgaataatt tgcgcttgag gaatatacag taaccgccaa
    10021 ttatggatgt gtaaacatct ggacggctaa atactaaaaa ttccataagg ggcatataat
    10081 gacaattctt aatcacaccc tcggtttccc tcgcgttggc ctgcgtcgcg agctgaaaaa
    10141 agcgcaagaa agttattggg cggggaactc cacgcgtgaa gaactgctga cagtagggcg
    10201 tgaactgcgt gctcgtcact gggatcaaca aaagcaagcg ggtatcgacc tgctgccggt
    10261 gggcgatttt gcctggtacg atcatgttct gaccaccagt ctgctgctgg gtaatgttcc
    10321 gccacgtcat cagaacaaag acggttcggt agatatcgac accctgttcc gtattggtcg
    10381 tggacgtgcg ccgactggcg aacctgcggc ggcagcggaa atgaccaaat ggtttaacac
    10441 caactatcac tacatggtgc cggaattcgt taaaggccaa cagttcaaac tgacctggac
    10501 gcagctgctg gaggaagtgg acgaggcgct ggcgctgggc cacaacgtta agcctgtgct
    10561 gctggggccg gttacttacc tgtggctggg gaaagtgaaa ggtgaacagt ttgaccgcct
    10621 tagcctgctg aacgacattc tgccggttta tcagcaagtg ctggcagaac tggcgaaacg
    10681 cggcatcgag tgggtacaga ttgatgaacc cgcgctggta ctggaactac cacaggcgtg
    10741 gctggacgca tacaaacccg cttacgacgc gctccaggga caggtgaaac tgctgctgac
    10801 cacctatttt gaaggcgtaa cgccaaatct cgacacgatt actgcgctgc ctgttcaggg
    10861 tctgcatgtt gacctcgtac atggtaaaga tgacgttgct gaactgcaca agcgcctgcc
    10921 ttctgactgg ttgctgtctg cgggtctgat caatggtcgt aacgtctggc gcgccgatct
    10981 taccgagaaa tatgcgcaaa ttaaggacat tgtcggcaaa cgtgatttgt gggtggcatc
    11041 ttcctgctcg ttgctgcaca gccccatcga cctgagcgtg gaaacgcgtc ttgatgcaga
    11101 agtgaaaagc tggtttgcct tcgccctaca aaaatgccat gaactggcac tgctgcgcga
    11161 tgcgctgaac agtggtgaca cggcagctct ggcagagtgg agcgccccga ttcaggcacg
    11221 tcgtcactct acccgcgtac ataatccggc ggtagaaaag cgtctggcgg cgatcaccgc
    11281 ccaggacagc cagcgtgcga atgtctatga agtgcgtgcc gaagcccagc gtgcgcgttt
    11341 taaactgcca gcgtggccga ccaccacgat tggttctttc ccgcaaacca cggaaattcg
    11401 taccctgcgt ctggatttca aaaagggcaa tctcgacgcc aataactacc gcacgggcat
    11461 tgcggaacat atcaggcagg ccattgttga acaggaacgt ttgggactgg atgtgctggt
    11521 acatggcgag gccgagcgta atgacatggt ggaatacttt ggcgagcacc tcgacgggtt
    11581 tgtctttacg caaaacggtt gggtacagag ctacggttcc cgctgcgtga agccaccgat
    11641 tgttattggt gacgttagtc gcccggcacc gattaccgtg gagtgggcga agtatgcgca
    11701 atcgctgacc gacaaaccgg tgaaagggat gctgacgggg ccggtgacca tactctgctg
    11761 gtcgttcccg cgtgaagatg tcagccgtga aaccatcgcc aaacagattg cgctggcgct
    11821 gcgtgatgag gtggccgatc tggaagcggc agggattggt atcatccaga ttgacgaacc
    11881 ggcgctgcgc gaaggtttac cactgcgtcg tagcgactgg gacgcctatc tccagtgggg
    11941 cgtagaggcc ttccgtatca acgccgccgt ggcgaaagat gacacacaaa tccacactca
    12001 catgtgttat tgcgagttca acgacatcat ggattccatt gcggcgctgg atgcagacgt
    12061 catcaccatc gaaacctcgc gttccgacat ggagttgctg gagtcgtttg aagagtttga
    12121 ttatccgaat gaaatcggtc caggcgtcta tgacattcac tcgccaaacg taccgagcgt
    12181 ggaatggatt gaagccctgc tgaagaaagc ggcaaaacgc attccggcag agcgcctgtg
    12241 ggtcaaccca gactgtggcc tgaaaacgcg cggctggcca gaaacccgcg cggcactggc
    12301 gaacatggtg caggcggcgc agaatttgcg tctggggtaa atccaaaccg ggtggtaata
    12361 ccacccggtc ttttctcatt acagcgactt cttcccacca tactgcttaa accattccag
    12421 catacgctgc cagccatctt tcgcagattc ggcatgatag ctcgggcgat aatcagcgtt
    12481 gaatgcatgc ccggcatccg ggtagacgat aatctctgct ttcgcattag cagcccgcag
    12541 cgcctggcgc atcgtttcaa cgctctcctg cggaatgctg ttatcctgac cgccatataa
    12601 gccgagaacc ggcgcgttaa gatcggttgc gatatcaaca ggttgtttcg gtgaattcag
    12661 cgacttatcg cccgtcagtt tgccgtacca cgccactgcg gcttttagct gtggattatg
    12721 cgcggcatac agccaggtga tacgtccacc ccagcagaat ccggtgatca ttaaacgatg
    12781 aacatcgccg ccgttgcggg aagcccaact ggcgacatga tcgagatcgg ccagcacctg
    12841 cgagtcaggc acttttgcta ccagaccgct aagcaacgtg gggatatcgg caaaatcatt
    12901 cggatcgcct tcgcggaagt aaagttcagg tgcgatagcc agatacccct ccagcgccag
    12961 acggcgacaa atgtcgcgga tatgttcatg cacgccaaaa atttcctgca ctacaatgac
    13021 cactggcagt gggccatcgc tttgctttgg tctggcatgg taagcaggca tgttatcccc
    13081 ttgtgaaggg atagaggtga agcccgccac aatcgcgtcg tccggggtct gaacgatggt
    13141 cgaagcgaga ggcgatgcag caggtgcaaa tccagattgt tgtgttgttg ccatggtatt
    13201 ctccgtaccc ttataaaaat gttgcgcaat gttaactata gtcagcatgc aacaaatcac
    13261 attgcctgaa tcggctcatc ttttatgcag tcctgcagaa tgaagagtga tttatgtgat
    13321 ttgcatcact tttggtgggt aaatttatgc aacgcatttg cgtcatggtg atgagtatca
    13381 cgaaaaaatg ttaaaccctt cggtaaagtg tctttttgct tcttctgact aaaccgattc
    13441 acagaggagt tgtatatgtc caagtctgat gtttttcatc tcggcctcac taaaaacgat
    13501 ttacaagggg ctacgcttgc catcgtccct ggcgacccgg atcgtgtgga aaagatcgcc
    13561 gcgctgatgg ataagccggt taagctggca tctcaccgcg aattcaccac ctggcgtgca
    13621 gagctggatg gtaaacctgt tatcgtctgc tctaccggta tcggcggccc gtctacctct
    13681 attgctgttg aagagctggc acagctgggc attcgcacct tcctgcgtat cggtacaacg
    13741 ggcgctattc agccgcatat taatgtgggt gatgtcctgg ttaccacggc gtctgtccgt
    13801 ctggatggcg cgagcctgca cttcgcaccg ctggaattcc cggctgtcgc tgatttcgaa
    13861 tgtacgactg cgctggttga agctgcgaaa tccattggcg cgacaactca cgttggcgtg
    13921 acagcttctt ctgatacctt ctacccaggt caggaacgtt acgatactta ctctggtcgc
    13981 gtagttcgtc actttaaagg ttctatggaa gagtggcagg cgatgggcgt aatgaactat
    14041 gaaatggaat ctgcaaccct gctgaccatg tgtgcaagtc agggcctgcg tgccggtatg
    14101 gtagcgggtg ttatcgttaa ccgcacccag caagagatcc cgaatgctga gacgatgaaa
    14161 caaaccgaaa gccatgcggt gaaaatcgtg gtggaagcgg cgcgtcgtct gctgtaattc
    14221 tcttctcctg tctgaaggcc gacgcgttcg gccttttgta tttttgcgta gcgcctcgca
    14281 ggaaatgcct ttccaactgg acgtttgtac agcacaattc tattttgtgc gggtaagttg
    14341 ttgcgtcagg aggcgttgtg gatttctcaa tcatggttta cgcagttatt gcgttggtgg
    14401 gtgtggcaat tggctggctg tttgccagtt atcaacatgc gcagcaaaaa gccgagcaat
    14461 tagctgaacg tgaagagatg gtcgcggagt taagcgcggc aaaacaacaa attacccaaa
    14521 gcgagcactg gcgtgcagag tgcgagttac tcaataacga agtgcgcagc ctgcaaagta
    14581 ttaacacctc tctggaggcc gatctgcgtg aagtaaccac gcggatggaa gccgcacagc
    14641 aacatgctga cgataaaatt cgccagatga ttaacagcga gcagcgcctc agtgagcagt
    14701 ttgaaaacct cgccaaccgt atttttgagc acagcaatcg ccgggttgat gagcaaaacc
    14761 gtcagagtct gaacagcctg ttgtcgccgc tacgtgaaca actggacggt ttccgccgtc
    14821 aggttcagga cagcttcggt aaagaagcac aagaacgcca taccctgacc cacgaaattc
    14881 gcaatctcca gcaactcaac gcgcaaatgg cccaggaagc gatcaacctg acgcgcgcgc
    14941 tgaaaggcga caataaaacc cagggcaact ggggcgaggt agtattgacg cgggtgctgg
    15001 aggcttccgg tctgcgtgaa gggtatgaat atgaaaccca ggtcagcatc gaaaatgacg
    15061 cccgctcgcg gatgcagccg gatgtcatcg tgcggctgcc gcagggaaaa gatgtggtga
    15121 tcgacgccaa aatgacgctg gtcgcctatg aacgctattt taacgccgaa gacgactaca
    15181 cccgcgaaag cgcgctacag gaacatatcg cgtcggtgcg taaccatatc cgtttgctgg
    15241 gacgcaaaga ttatcaacag ctgccggggc tgcgaactct ggattacgtg ctgatgttta
    15301 ttcccgttga acccgctttt tcactggcgc ttgaccgcca gccggagctg atcaccgaag
    15361 cgttgaaaaa caatatcatg ctggttagcc cgactacgct gctggtggcg ttgcgtacta
    15421 ttgccaacct gtggcgttat gagcatcaaa gccgcaacgc ccagcaaatc gccgatcgtg
    15481 ccagcaagct gtacgacaag atgcgtttgt tcatcgatga catgtccgcg attggtcaaa
    15541 gtctcgacaa agcgcaggat aactaccgcc aggcaatgaa aaaactctct tcaggtcgcg
    15601 gaaatgtgct ggcgcaggca gaagcgtttc gcggtttagg agtagaaatt aaacgcgaga
    15661 ttaatccgga tttggctgaa caggcggtga gccaggatga agagtatcga cttcggtcgg
    15721 ttccggagca gccgaatgat gaagcttatc aacgcgatga tgaatataat cagcagtcgc
    15781 gctagcccat taggggtagt taagccgggt agaaatctag ggcatcgacg cccaatctgt
    15841 tacacttctg gaacaatttt ttgatgagca ggcattgaga tggtggataa gtcacaagaa
    15901 acgacgcact ttggttttca gaccgtcgcg aaggaacaaa aagcggatat ggtcgcccac
    15961 gttttccatt ccgtggcatc aaaatacgat gtcatgaatg atttgatgtc atttggtatt
    16021 catcgtttgt ggaagcgatt cacgattgat tgcagcggcg tacgccgtgg gcagaccgtg
    16081 ctggatctgg ctggtggcac cggcgacctg acagcgaaat tctcccgcct ggtcggagaa
    16141 actggcaaag tggtccttgc tgatatcaat gaatccatgc tcaaaatggg acgcgagaag
    16201 ctgcgtaata tcggtgtgat tggcaacgtt gagtatgttc aggcgaacgc tgaggcgctg
    16261 ccgttcccgg ataacacctt tgattgcatc accatttcgt ttggtctgcg taacgtcacc
    16321 gacaaagata aagcactgcg ttcaatgtat cgcgtgctga aacccggcgg ccgcctgctg
    16381 gtgcttgagt tctcgaagcc aattatcgag ccgctgagca aagcctatga tgcatactcc
    16441 ttccatgtgc tgccgcgtat tggctcactg gtcgcgaacg acgccgacag ctaccgttat
    16501 ctggcagaat ccatccgtat gcatcccgat caggataccc tgaaagccat gatgcaggat
    16561 gccggattcg aaagtgtcga ctactacaat ctgacggcgg gggttgtggc gctgcatcgt
    16621 ggttataagt tctgacagga gaccggaaat gccttttaaa cctttagtga cggcaggaat
    16681 tgaaagtctg ctcaacacct tcctgtatcg ctcacccgcg ctgaaaacag cccgctcgcg
    16741 tctgctgggt aaagtattgc gcgtggaggt aaaaggcttt tcgacgtcat tgattctggt
    16801 gttcagcgaa cgccaggttg atgtactggg cgaatgggca ggcgatgctg actgcaccgt
    16861 tatcgcctac gccagtgtgt tgccgaaact tcgcgatcgc cagcagctta ccgcactgat
    16921 tcgcagtggt gagctggaag tgcagggcga tattcaggtg gtgcaaaact tcgttgcgct
    16981 ggcagatctg gcagagttcg accctgcgga actgctggcc ccttataccg gtgatatcgc
    17041 cgctgaagga atcagcaaag ccatgcgcgg aggcgcaaag ttcctgcatc acggcattaa
    17101 gcgccagcaa cgttatgtgg cggaagccat tactgaagag tggcgtatgg cacccggtcc
    17161 gcttgaagtg gcctggtttg cggaagagac ggctgccgtc gagcgtgctg ttgatgccct
    17221 gaccaaacgg ctggaaaaac tggaggctaa atgacgccag gtgaagtacg gcgcctatat
    17281 ttcatcattc gcactttttt aagctacgga cttgatgaac tgatccccaa aatgcgtatc
    17341 accctgccgc tacggctatg gcgatactca ttattctgga tgccaaatcg gcataaagac
    17401 aaacttttag gtgagcgact acgactggcc ctgcaagaac tggggccggt ttggatcaag
    17461 ttcgggcaaa tgttatcaac ccgccgcgat ctttttccac cgcatattgc cgatcagctg
    17521 gcgttattgc aggacaaagt tgctccgttt gatggcaagc tggcgaagca gcagattgaa
    17581 gctgcaatgg gcggcttgcc ggtagaagcg tggtttgacg attttgaaat caagccgctg
    17641 gcttctgctt ctatcgccca ggttcatacc gcgcgattga aatcgaatgg taaagaggtg
    17701 gtgattaaag tcatccgccc ggatattttg ccggtcatta aagcggatct gaaacttatc
    17761 taccgtctgg ctcgctgggt gccgcgtttg ctgccggatg gtcgccgtct gcgcccaacc
    17821 gaagtggtgc gcgagtacga aaagaccttg attgatgaac tgaatttgct gcgggaatct
    17881 gccaacgcca ttcagcttcg gcgcaatttt gaagacagcc cgatgctcta catcccggaa
    17941 gtttaccctg actattgtag tgaagggatg atggtgatgg agcgcattta cggcattccg
    18001 gtgtctgatg ttgcggcgct ggagaaaaac ggcactaaca tgaaattgct ggcggaacgc
    18061 ggcgtgcagg tgttcttcac tcaggtcttt cgcgacagct ttttccatgc cgatatgcac
    18121 cctggcaaca tcttcgtaag ctatgaacac ccggaaaacc cgaaatatat cggcattgat
    18181 tgcgggattg ttggctcgct aaacaaagaa gataaacgct atctggcaga aaactttatc
    18241 gccttcttta atcgcgacta tcgcaaagtg gcagagctac acgtcgattc aggctgggtg
    18301 ccaccagata ccaacgttga agagttcgaa tttgccattc gtacggtctg tgaacctatc
    18361 tttgagaaac cgctggccga aatttcgttt ggacatgtac tgttaaatct gtttaatacg
    18421 gcgcgtcgct tcaatatgga agtgcagccg caactggtgt tactccagaa aaccctgctc
    18481 tatgtcgaag gggtaggacg ccagctttat ccgcagctcg atttatggaa aacagcgaag
    18541 cctttcctgg agtcgtggat taaagatcag gtcggtattc ctgcgctggt gagagcattt
    18601 aaagaaaaag cgccgttctg ggtcgaaaaa atgccagaac tgcctgaatt ggtttacgac
    18661 agtttgcgcc agggcaagta tttacagcac agtgttgata agattgcccg cgagcttcag
    18721 tcaaatcatg tacgtcaggg acaatcgcgt tattttctcg gaattggcgc tacgttagta
    18781 ttaagtggca cattcttgtt ggtcagccga cctgagtggg ggctgatgcc cggctggtta
    18841 atggcaggtg gtctgatcgc ctggtttgtc ggttggcgca aaacacgctg attttttcat
    18901 cgctcaaggc gggccgtgta acgtataatg cggctttgtt taatcatcat ctaccacaga
    18961 ggaacatgta tgggtggtat cagtatttgg cagttattga ttattgccgt catcgttgta
    19021 ctgctttttg gcaccaaaaa gctcggctcc atcggttccg atcttggtgc gtcgatcaaa
    19081 ggctttaaaa aagcaatgag cgatgatgaa ccaaagcagg ataaaaccag tcaggatgct
    19141 gattttactg cgaaaactat cgccgataag caggcggata cgaatcagga acaggctaaa
    19201 acagaagacg cgaagcgcca cgataaagag caggtgtaat ccgtgtttga tatcggtttt
    19261 agcgaactgc tattggtgtt catcatcggc ctcgtcgttc tggggccgca acgactgcct
    19321 gtggcggtaa aaacggtagc gggctggatt cgcgcgttgc gttcactggc gacaacggtg
    19381 cagaacgaac tgacccagga gttaaaactc caggagtttc aggacagtct gaaaaaggtt
    19441 gaaaaggcga gcctcactaa cctgacgccc gaactgaaag cgtcgatgga tgaactacgc
    19501 caggccgcgg agtcgatgaa gcgttcctac gttgcaaacg atcctgaaaa ggcgagcgat
    19561 gaagcgcaca ccatccataa cccggtggtg aaagataatg aagctgcgca tgagggcgta
    19621 acgcctgccg ctgcacaaac gcaggccagt tcgccggaac agaagccaga aaccacgcca
    19681 gagccggtgg taaaacctgc tgcggacgct gaaccgaaaa ccgctgcacc ttccccttcg
    19741 tcgagtgata aaccgtaaac atgtctgtag aagatactca accgcttatc acgcatctga
    19801 ttgagctgcg taagcgtctg ctgaactgca ttatcgcggt gatcgtgata ttcctgtgtc
    19861 tggtctattt cgccaatgat atctatcacc tggtatccgc gccattgatc aagcagttgc
    19921 cgcaaggttc aacgatgatc gccaccgacg tggcctcgcc gttctttacg ccgatcaagc
    19981 tgacctttat ggtgtcgctg attctgtcag cgccggtgat tctctatcag gtgtgggcat
    20041 ttatcgcccc agcgctgtat aagcatgaac gtcgcctggt ggtgccgctg ctggtttcca
    20101 gctctctgct gttttatatc ggtatggcat tcgcctactt tgtggtcttt ccgctggcat
    20161 ttggcttcct tgccaatacc gcgccggaag gggtgcaggt atccaccgac atcgccagct
    20221 atttaagctt cgttatggcg ctgtttatgg cgtttggtgt ctcctttgaa gtgccggtgg
    20281 caattgtgct gctgtgctgg atggggatta cctcgccaga agacttacgc aaaaaacgcc
    20341 cgtatgtgct ggttggtgca ttcgttgtcg ggatgttgct gacgccgccg gatgtcttct
    20401 cgcaaacgct attggcgatc ccgatgtact gtctgtttga aatcggtgtc ttcttctcac
    20461 gcttttacgt tggtaaaggg cgaaatcggg aagaggaaaa cgacgctgaa gcagaaagcg
    20521 aaaaaactga agaataaatt caaccgcccg tcagggcggt tgtcatatgg agtacaggat
    20581 gtttgatatc ggcgttaatt tgaccagttc gcaatttgcg aaagaccgtg atgatgttgt
    20641 agcgcgcgct tttgacgcgg gagttaatgg gctactcatc accggcacta acctgcgtga
    20701 aagccagcag gcgcaaaagc tggcgcgtca gtattcgtcc tgttggtcaa cggcgggcgt
    20761 acatcctcac gacagcagcc agtggcaagc tgcgactgaa gaagcgatta ttgagctggc
    20821 cgcgcagcca gaagtggtgg cgattggtga atgtggtctc gactttaacc gcaacttttc
    20881 gacgccggaa gagcaggaac gcgcttttgt tgctcagcta cgcattgccg cagaattaaa
    20941 catgccggta tttatgcact gtcgcgatgc ccacgagcgg tttatgacat tgctggagcc
    21001 gtggctggat aaactgcctg gtgcggttct tcattgcttt accggcacac gcgaagagat
    21061 gcaggcgtgc gtggcgcgtg gaatttatat cggcattacc ggttgggttt gcgatgaacg
    21121 acgcgggctg gagctgcggg aattgttgcc gttgattccg gcggagaaat tgctgatcga
    21181 aactgatgcg ccgtatctgc tcccgcgcga tctcacgcca aagccatcat cccggcgcaa
    21241 cgagccagcc catctgcccc atattttgca acgtattgcg cactggcgtg gagaagatgc
    21301 cgcatggctg gctgccacca cggatgctaa tgtcaaaaca ctgtttggga ttgcgtttta
    21361 gagtttgcgg aactcggtgt tcttcacact gtgcttaatc tctttattaa taagattaag
    21421 caatagcatg gagcgagcct caccatcggg ttcggtgaaa atggcctgaa agccttcgaa
    21481 cgcgccttcg gtaataatca ccttatcacc cggataaggg gttgccggat cgacaatgtc
    21541 tttcggttta tataccgata gctgatgaat caccgccgat gggactatcg ctggcgacgc
    21601 gccaaagcgc acgaagtggc tgacaccgcg ggtcgcgttg atagtcgtgg tatgaatcac
    21661 ttctggatca aattcgacaa acaggtagtt gggaaacaat ggctcactga ctgcagtacg
    21721 ttttccacgc acgatttttt ccagggtgat catcggtgcc aggcaattca cagcctgtct
    21781 ttcgaggtgt tcctgggcac gttgaagttg cccgcgcttg cagtacagta aataccagga
    21841 ttgcataatg actcttatcc gtttaatcgg ggcgcaagga tagcaaaagc tttacgctaa
    21901 gttaattata ttccccggtt tgcgttatac cgtcagagtt cacgctaatt taacaaattt
    21961 acagcatcgc aaagatgaac gccgtataat gggcgtagat taagaggcta caatggacgc
    22021 catgaaatat aacgatttac gcgacttctt gacgctgctt gaacagcagg gtgagctaaa
    22081 acgtatcacg ctcccggtgg atccgcatct ggaaatcact gaaattgctg accgcacttt
    22141 gcgtgccggt gggcctgcgc tgttgttcga aaaccctaaa ggctactcaa tgccggtgct
    22201 gtgcaacctg ttcggtacgc caaagcgcgt ggcgatgggc atggggcagg aagatgtttc
    22261 ggcgctgcgt gaagttggta aattattggc gtttctgaaa gagccggagc cgccaaaagg
    22321 tttccgcgac ctgtttgata aactgccgca gtttaagcaa gtattgaaca tgccgacaaa
    22381 gcggctgcgt ggtgcgccct gccaacaaaa aatcgtctct ggcgatgacg tcgatctcaa
    22441 tcgcattccc attatgacct gctggccgga agatgccgcg ccgctgatta cctgggggct
    22501 gacagtgacg cgcggcccgc ataaagagcg gcagaatctg ggcatttatc gccagcagct
    22561 gattggtaaa aacaaactga ttatgcgctg gctgtcgcat cgcggcggcg cgctggatta
    22621 tcaggagtgg tgtgcggcgc atccgggcga acgtttcccg gtttctgtgg cgctgggtgc
    22681 cgatcccgcc acgattctcg gtgcagtcac ccccgttccg gatacacttt cagagtatgc
    22741 gtttgccgga ttgctgcgcg gtaccaagac cgaagtggtg aagtgtatct ccaatgacct
    22801 tgaagtgccc gccagtgcgg agattgtgct ggaagggtat atcgaacaag gtgaaacggc
    22861 gccggaaggg ccgtatggcg accacaccgg ttactataac gaagtcgata gtttcccggt
    22921 atttaccgtg acgcatatta cccagcgtga agatgcgatt taccattcca cctataccgg
    22981 gcgtccgcca gatgagcccg cggtactggg cgtggcgttg aacgaagtgt ttgtaccgat
    23041 tctgcaaaaa cagttcccgg aaattgtcga tttttacctg ccgccggaag gctgttctta
    23101 tcgtctggcg gtagtgacga tcaaaaaaca gtacgccgga cacgcgaagc gcgtcatgat
    23161 gggcgtctgg tcgttcttac gccagtttat gtacactaaa tttgtgatcg tttgcgatga
    23221 tgacgtcaac gcacgcgact ggaacgatgt gatttgggcg attaccaccc gtatggaccc
    23281 ggcgcgggat actgttctgg tagaaaatac gcctattgat tatctggatt ttgcctcgcc
    23341 tgtctccggg ctgggttcaa aaatggggct ggatgccacg aataaatggc cgggggaaac
    23401 ccagcgtgaa tggggacgtc ccatcaaaaa agatccagat gttgtcgcgc atattgacgc
    23461 catctgggat gaactggcta tttttaacaa cggtaaaagc gcctgatgcg cgtttgtttt
    23521 gccctattta tcgatccgac agagaaagcg catgacaacc ttaagctgta aagtgacctc
    23581 ggtagaagct atcacggata ccgtatatcg tgtccgcatc gtgccagacg cggccttttc
    23641 ttttcgtgct ggtcagtatt tgatggtagt gatggatgag cgcgacaaac gtccgttctc
    23701 aatggcctcg acgccggatg aaaaagggtt tatcgagctg catattggcg cttctgaaat
    23761 caacctttac gcgaaagcag tcatggaccg catcctcaaa gatcatcaaa tcgtggtcga
    23821 tattccccac ggagaagcgt ggctgcgcga tgatgaagag cgtccgatga ttttgattgc
    23881 gggcggcacc gggttctctt atgcccgctc gattttgctg acagcgttgg cgcgtaaccc
    23941 aaaccgtgat atcaccattt actggggcgg gcgtgaagag cagcatctgt atgatctctg
    24001 cgagcttgag gcgctttcgt taaagcaccc tggtctgcaa gtggtgccgg tggttgaaca
    24061 accggaagca ggctggcgtg ggcgtactgg taccgtgtta acggcggtat tgcaggatca
    24121 tggtacgctg gcagagcatg atatctatat tgccggacgt tttgagatgg cgaaaattgc
    24181 ccgtgacctg ttttgcagtg agcgtaatgc gcgggaagat cgcctgtttg gcgatgcgtt
    24241 tgcatttatc tgagatataa aaaaacccgc ccctgacagg cgggaagaac ggcaactaaa
    24301 actttattca gtggcattta gatctatgtc gcatccggca atcggtgcac gatgcctgat
    24361 gcgacgctgg cgcgtcttat caggcctaca ttggtgcagg atcggtagac cggataaggc
    24421 gttcacgccg catccggcaa gtggttaaac ccgctcaaac accgtcgcaa taccctgacc
    24481 cagaccgata cacatcgtcg ccagaccaaa ctgaacgtct ttgcgttcca tcagattcag
    24541 cagcgtggtg ctgatacgcg caccggaaca gcccagcgga tgacccagcg cgatcgcacc
    24601 accgttgagg ttgatcttct cgtcaatctg ctcaatcagt cccagatctt taatacatgg
    24661 caggatctgc gcggcaaagg cttcgttcat ctcaaacacg ccgatatcgc tggcagaaag
    24721 ccccgctttt ttcagcgcca gcttcgaggc cggaaccggg ccgtaaccca taatcgatgg
    24781 gtcacaacca acgaccgcca tcgaacgcac acgagcgcgc ggcttaagac ctaattcatg
    24841 ggcgcggctt tcactcatca ccagcatggc agctgcgcca tcggaaagtg cagaagatgt
    24901 gcccgccgtt accgtaccgt ttactggatc aaacgccgga cgcagcgtgg cgagggcttc
    24961 cacggtggtt tccgggcgaa tcacttcgtc gtaattaaac tgcttcagga cgccgtcggc
    25021 atcgtgacca ccagttggga tgatttcatt tttaaatgcg gccgactgcg tggcggccca
    25081 ggcgcgggcg tgtgaccgcg cggcaaaggc atcctgcatt tcacggctga taccgtgcat
    25141 acgcgccagc atttctgctg ttaagcccat catgcccgcc gctttggcga cattgcggct
    25201 caggccgggg tgaaaatcga cgccgtgact catcggcaca tggcccatat gctccacgcc
    25261 gccaaccaga catgcctgcg catcgccggt cataatcatc cgtgctgcgt catgcagtgc
    25321 ctgcatggat gaaccacaca agcgattgac ggtaaccgcc gggacagagt gtggtacttc
    25381 tgccagcagc gccgcgttac gggcgatgtt aaaaccctgc tccagcgtct gctgcacaca
    25441 accccagtaa atatcgtcga gggccgccgc ttccagcgcc gggttacgcg ccagcaggct
    25501 acgcattaaa tgagcggaga gatcttctgc acgcacgtta cgaaaagcac cgcccttcga
    25561 acggcccatc ggggtgcgaa ttgcatcgac aatgacaacc tgttccattg tgactcctta
    25621 agccgttttc aggtcgccaa ccggacgggc tggctcaacc ggaggatagt acggttcgtt
    25681 atgacgcgct ttattacgca gaccttccgg cacttcatac agcgggccga ggtgctgata
    25741 ttgctgtgcc atatcgaggt attttgcgct accgagggtg tccagccagc ggaacgcgcc
    25801 gccgtggaac ggagggaagc ccaggccgta gaccagcgcc atatccgctt ccgccggagt
    25861 ggcgataatg ccttcctcca gacagcgcac cacttcgttg accatcggga tcatcatgcg
    25921 ggcgataatc tcctcttcgc tgaaatcgcg cttcggctgg ctcacttctg ccagcaggtc
    25981 ttcaacggcg gcgtcttctt ctttcttcgg cttacctttg ctgtcttctt tataacgcca
    26041 gaaaccgagg ccgttcttct gaccaaagcg gttggcatca aacagcgcgt cgatggcatc
    26101 gcggtaatct ttctgcatcc tctgcgggaa gcctgctgcc atgacagcct gggcatgatg
    26161 cgcggtgtca atgcccacga catccagcag atatgccgga cccatcggcc agccaaactg
    26221 tttttccatc actttgtcga tcttgcggaa atccgcgccg tcgcgcagca gctggctgaa
    26281 accggcgaaa tacgggaaca gcacgcggtt aacaaagaag ccggggcagt cgttaaccac
    26341 aatcggcgtc ttgcccatct tgctcgccca ggcgacaact ttcgcgatgg tttcgtcgga
    26401 gcttttctcg ccgcgaataa tttctaccaa cggcattcgg tggaccgggt taaagaagtg
    26461 catcccgcag aagttttccg ggcgtttcag cgcgttggcc agttcgctga taggaatggt
    26521 tgaagtgtta gacgccagca cggtatcctg gcgtactttt tgttcggttt ctgccagtac
    26581 ggcttttttc actttcgggt tttcaacaac cgcttctacc acaatatcca cgcggtcaaa
    26641 tccggcgtag tcgagcgttg ggtggattgt ggagatcacg ccagccagtt tcagaccatc
    26701 gatcttgccg cgctcaagct gcttgttcag cagtttcgcg gcttcggtca tgccgagggt
    26761 taacgacttg tcgttgatat ctttcatgac aaccggcacg cctttccacg cagactggta
    26821 agcgatgccg ccgcccataa tgcctgcacc cagcaccgcg gcctgtttcg gggtttcaac
    26881 gtctttggtg agtttcttcg ctttgccttt tacatattga tcgttaagga aaatgccgac
    26941 cagtgcgcgg gcttcgttgg tatgcgccag cgggacaaaa cttttgtttt ccaggtttaa
    27001 ggcttcttca cgaccaaaac gggccgcagc ttcaatggtt tttactgcgg tgatgggggc
    27061 cggataatgt ttccccgctg tttgtgcgac catcccttta gcgatggtga agctcatggt
    27121 ggcttcaatc ttgctcagtt ttagtggttc cagcttcggc tgacgttttg ctttccagtc
    27181 gaggtcgccg ttaatggcct ggcgtaaaac cgcctttgcg ccttcaacca gtttttctgc
    27241 tttgactacg ccatccacca gaccgatttt cagcgcctga tccgcgccga catctttacc
    27301 ggcggcaatg atttccagcg cactgtctgc gcccagcata cgtggcatac gtacagaacc
    27361 gccaaagccc ggcatgatgc ccagtttggt ttccggcaga ccgatgcgca gatccggcgt
    27421 cgccagacga taatcggtcg ccagcacgca ttcgcagcca ccgcccagcg catagccgtt
    27481 gacggcagca atggtcggca ccggcagatc ttccaggcga ttaaacacgc tattggcaaa
    27541 gtgcagccac tgacttaact gttcttcagg aacgaggaac agggacaaaa attcggtgat
    27601 atcagcaccg acgataaagg ctgctttgtt cgaacgcagc agcagccctt ttagatctga
    27661 ttgctgttcc agcacgccga tggcctcgcc gaggctggcg acggtcgcag tgtcgagttt
    27721 attaactgaa cctggggcat caaataccag ttcggcaatg ccatcttcca gccagtcaag
    27781 gtacagggtg tcgcctttgt aaagcatgtc agtctcctga atccgcaagg tgatctggtc
    27841 gtaccagatg catcgaagtg tgtattttgt gttaaaaata tgcaaataaa agattaaaga
    27901 aatgccgatc tgatcacgct cggcagaaat cacgctctgg atgaacgatg tgctaagatg
    27961 cggagactta aggtcaaaaa aacagaaggg taaaaaatgg aatcactggc ctcactctat
    28021 aaaaatcata tagctacctt acaggaacgg actcgcgatg cgctggcgcg cttcaagctg
    28081 gatgcgttac ttattcactc cggcgaactg ttcaacgttt ttctcgacga tcatccctat
    28141 ccgtttaaag tgaacccgca attcaaagcg tgggtaccgg taactcaggt gccaaactgc
    28201 tggctgctgg tggatggcgt gaacaagccg aaactgtggt tctatctgcc ggttgattac
    28261 tggcacaacg tcgaaccgct gccgacctct ttctggactg aagacgtgga agtgatcgcg
    28321 ctgccgaaag ccgatggcat tggtagcctg ctgcctgctg cgcgcgggaa tatcggttat
    28381 atcggtccgg tgccggaacg tgcgctgcaa ctgggtattg aggccagcaa tatcaacccg
    28441 aaaggggtga tcgactacct gcattactac cgctccttca aaaccgagta cgaactggcc
    28501 tgtatgcgtg aagcgcagaa aatggcggtc aacggtcatc gcgcggcaga agaagcgttc
    28561 cgttctggca tgagcgagtt cgatatcaac attgcctatc tgaccgcgac cggtcatcgt
    28621 gataccgacg taccttacag caacattgtg gcgctcaacg aacacgctgc ggtgttgcat
    28681 tacaccaaac tggaccacca ggcaccggaa gagatgcgca gcttcctgct ggatgccggg
    28741 gccgaatata acggctatgc cgctgacctg acccgtacct ggtcggcaaa aagtgacaac
    28801 gattacgcac agctggtgaa agacgtaaat gatgaacaac tggcgctgat cgcgaccatg
    28861 aaagctggcg ttagctatgt ggattaccac atccagttcc atcagcgcat cgccaaactg
    28921 ctgcgtaaac atcaaatcat caccgatatg agtgaagaag cgatggtcga aaacgatctc
    28981 accggaccgt ttatgccgca cggtatcggc catccgctgg gcctgcaggt gcatgacgtc
    29041 gccggtttta tgcaggatga tagcggtacg cacctcgcgg caccggcaaa atatccgtac
    29101 ctgcgctgca cccgtattct ccagccgggc atggtgttaa ccatcgaacc gggtatctac
    29161 ttcattgaat cgctactggc accgtggcgt gaagggcagt tcagcaagca cttcaactgg
    29221 cagaaaattg aagcactgaa accgttcggc ggcattcgta tcgaagacaa cgtggtgatc
    29281 cacgaaaata acgtggaaaa catgacccgg gatctgaaac tggcgtgatg gaaagctggt
    29341 taattcctgc ggcaccggtc acggtcgttg aagagatcaa aaagagccgc ttcataacga
    29401 tgttggcgca taccgatggc gttgaggcgg cgaaagcgtt tgttgaatcg gtgcgggcag
    29461 aacaccccga tgcccgccac cattgcgtgg cgtgggtggc gggtgcgccg gatgattcac
    29521 aacagctggg tttctctgac gacggggagc cggcgggaac ggcaggtaaa ccgatgctcg
    29581 cccagctaat gggcagcggc gttggggaaa ttaccgctgt ggtggtgcgc tactacggcg
    29641 gcatattgct tggcaccggt gggttagtga aagcgtatgg cggcggcgtg aatcaggcgc
    29701 tgcgccagct aacgacccaa cgcaagacgc cattaaccga atatactttg caatgtgaat
    29761 atcatcagtt aaccggcatt gaagcgttgc tggggcagtg tgacggcaaa attatcaaca
    29821 gtgattatca ggcattcgtt ctgctgcggg tggcgcttcc ggcggcgaaa gtggctgaat
    29881 tttccgcaaa gctggcggat tttagccgtg gttcattgca attgttagcg attgaagaat
    29941 aatccccact tcgttttgca gaactaagga agcggcagag atgcattttc gcgccattac
    30001 ccgaatcgtt ggactactgg tcatcttatt ttcagggacc atgattatcc ctgggctggt
    30061 agcactcatc taccgggatg gagcgggccg cgcttttacc cagacctttt ttgtcgccct
    30121 cgccattggc tctatgctgt ggtggccgaa ccgcaaagag aaaggcgaac ttaaatcccg
    30181 tgaggggttt ctgatagtgg tgctgttctg gaccgtgctg ggtagcgtcg gtgcgctccc
    30241 ttttatcttc tcggaaagcc cgaacctcac gattaccgat gcgttttttg aatctttctc
    30301 tggcctgacc accaccgggg ccactacgct ggtggggctg gattcgctcc ctcacgccat
    30361 cctcttttat cgccagatgc tgcaatggtt tggcgggatg gggatcatcg tgttagcggt
    30421 tgcgatactg cctatcctcg gcgtgggtgg gatgcagctc tatcgcgcag aaatgcccgg
    30481 cccgctgaaa gataacaaaa tgcgcccgcg aattgcggaa acggcgaaaa ccctgtggtt
    30541 gatttatgtc ttgctgaccg tcgcctgtgc gctggcgttg tggtttgccg gaatggatgc
    30601 ctttgatgcc atcggccata gctttgcgac tatcgctatt ggcggcttct cgacacatga
    30661 tgccagtatc ggttatttcg atagcccgac tattaacact atcattgcta tcttcctgct
    30721 gatctccggc tgtaactacg gtctgcactt ttcactgtta agtgggcgta gtctgaaggt
    30781 ttattggcgc gatccggaat ttcgcatgtt tatcggcgta cagtttacgc tggtggttat
    30841 ttgtaccctc gtactgtggt ttcataatgt ctacagttcg gcgctgatga caattaacca
    30901 ggcgtttttc ctggtggtgt cgatggcgac aaccgccggg tttacgactg acagcattgc
    30961 ccgctggccg ctctttttgc cggtactgct tttatgttcg gcttttatcg gcggttgtgc
    31021 cgggtcaacg ggcggtggcc tgaaagtgat ccgcatcctg ctgctgttta agcaggggaa
    31081 ccgtgagctg aaacgactgg tgcatccgaa cgccgtctat agcattaagc tggggaatcg
    31141 cgcactgccg gaacgtatcc tcgaagccgt ttggggattt ttctccgcct atgcattggt
    31201 gtttattgtc agtatgctgg cgattatcgc cacgggcgtg gatgactttt ctgcctttgc
    31261 ctcagttgtt gcgacattga ataacctggg gccagggctt ggcgtggttg ctgataactt
    31321 taccagtatg aacccggtgg ctaaatggat cctgattgcc aacatgctgt ttggtcgtct
    31381 cgaggtcttt acattgctgg tgctctttac cccgactttc tggcgtgaat gatggagtaa
    31441 tacgtgaaaa cattaattct tttctcaaca agggacggac aaacgcgcga gattgcctcc
    31501 tacctggctt cggaactgaa agaactgggg atccagacgg atgtcgccaa tgtgcaccgc
    31561 attgaagaac cacaatggga aaattatgac cgtgtggtca ttggtgcttc tattcgctat
    31621 ggtcactacc actctgcgtt ccaggaattt gtcaaaaaac atgcgacgcg actgaattcg
    31681 atgccgagcg ccttttattc cgtaaacctg gtggcgcgca aaccggagaa gcgtactcca
    31741 cagaccaaca gctacgcgcg gaagtttctg atgaactcgc aatggcgtcc cgatcgctgc
    31801 gcggtcattg ccggggcgct gcgttaccca cgttatcgct ggtacgaccg ttttatgatc
    31861 aagctgatta tgaagatgtc aggcggtgaa acggatacgc gcaaagaagt tgtctatacc
    31921 gattgggagc aggtggcgaa tttcgcccga gaaatcgccc atttaaccga caaaccgacg
    31981 ctgaaataag cacaaagaat aaaaaatgcg cggtcagaaa attattttaa atttcctctt
    32041 gtcaggccgg aataactccc tataatgcgc caccactgac acggaacaac ggcaaacacg
    32101 ccgccgggtc agcggggttc tcctgagaat tccgacagag aaagcaaaaa taaatgcttg
    32161 actctgtagc gggaaagcgt attatgcaca ccccgcg
//
LOCUS       DAZFWL020000044        30997 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFWL020000044 DAZFWL020000000
VERSION     DAZFWL020000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.044"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHKNP_RS10860"
                     /old_locus_tag="pgaptmp_002172"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHKNP_RS10860"
                     /old_locus_tag="pgaptmp_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGU4704335.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHKNP_RS10865"
                     /old_locus_tag="pgaptmp_002173"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHKNP_RS10865"
                     /old_locus_tag="pgaptmp_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGU4704336.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHKNP_RS10870"
                     /old_locus_tag="pgaptmp_002174"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHKNP_RS10870"
                     /old_locus_tag="pgaptmp_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGU4704337.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHKNP_RS10875"
                     /old_locus_tag="pgaptmp_002175"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHKNP_RS10875"
                     /old_locus_tag="pgaptmp_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGU4704338.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHKNP_RS10880"
                     /old_locus_tag="pgaptmp_002176"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHKNP_RS10880"
                     /old_locus_tag="pgaptmp_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGU4704339.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHKNP_RS10885"
                     /old_locus_tag="pgaptmp_002177"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHKNP_RS10885"
                     /old_locus_tag="pgaptmp_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGU4704340.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHKNP_RS10890"
                     /old_locus_tag="pgaptmp_002178"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHKNP_RS10890"
                     /old_locus_tag="pgaptmp_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGU4704341.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHKNP_RS10895"
                     /old_locus_tag="pgaptmp_002179"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHKNP_RS10895"
                     /old_locus_tag="pgaptmp_002179"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGU4704342.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHKNP_RS10900"
                     /old_locus_tag="pgaptmp_002180"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHKNP_RS10900"
                     /old_locus_tag="pgaptmp_002180"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGU4704343.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHKNP_RS10905"
                     /old_locus_tag="pgaptmp_002181"
     CDS             12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHKNP_RS10905"
                     /old_locus_tag="pgaptmp_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HGU4704344.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHKNP_RS10910"
                     /old_locus_tag="pgaptmp_002182"
     CDS             complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHKNP_RS10910"
                     /old_locus_tag="pgaptmp_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HGU4704345.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHKNP_RS10915"
                     /old_locus_tag="pgaptmp_002183"
     CDS             complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHKNP_RS10915"
                     /old_locus_tag="pgaptmp_002183"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HGU4704346.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHKNP_RS10920"
                     /old_locus_tag="pgaptmp_002184"
     CDS             complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHKNP_RS10920"
                     /old_locus_tag="pgaptmp_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HGU4704347.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHKNP_RS10925"
                     /old_locus_tag="pgaptmp_002185"
     CDS             complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHKNP_RS10925"
                     /old_locus_tag="pgaptmp_002185"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HGU4704348.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHKNP_RS10930"
                     /old_locus_tag="pgaptmp_002186"
     CDS             complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHKNP_RS10930"
                     /old_locus_tag="pgaptmp_002186"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGU4704349.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHKNP_RS10935"
                     /old_locus_tag="pgaptmp_002187"
     CDS             complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHKNP_RS10935"
                     /old_locus_tag="pgaptmp_002187"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HGU4704350.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19283..19383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHKNP_RS10940"
                     /old_locus_tag="pgaptmp_002188"
     CDS             complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHKNP_RS10940"
                     /old_locus_tag="pgaptmp_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HGU4704351.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20006..20779
                     /gene="nudC"
                     /locus_tag="ACHKNP_RS10945"
                     /old_locus_tag="pgaptmp_002189"
     CDS             20006..20779
                     /gene="nudC"
                     /locus_tag="ACHKNP_RS10945"
                     /old_locus_tag="pgaptmp_002189"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HGU4704352.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20819..21883
                     /gene="hemE"
                     /locus_tag="ACHKNP_RS10950"
                     /old_locus_tag="pgaptmp_002190"
     CDS             20819..21883
                     /gene="hemE"
                     /locus_tag="ACHKNP_RS10950"
                     /old_locus_tag="pgaptmp_002190"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HGU4704353.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21893..22564
                     /gene="nfi"
                     /locus_tag="ACHKNP_RS10955"
                     /old_locus_tag="pgaptmp_002191"
     CDS             21893..22564
                     /gene="nfi"
                     /locus_tag="ACHKNP_RS10955"
                     /old_locus_tag="pgaptmp_002191"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HGU4704354.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHKNP_RS10960"
                     /old_locus_tag="pgaptmp_002192"
     CDS             22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHKNP_RS10960"
                     /old_locus_tag="pgaptmp_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HGU4704355.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGDSNIGIIFQQ"
     gene            23384..23656
                     /gene="hupA"
                     /locus_tag="ACHKNP_RS10965"
                     /old_locus_tag="pgaptmp_002193"
     CDS             23384..23656
                     /gene="hupA"
                     /locus_tag="ACHKNP_RS10965"
                     /old_locus_tag="pgaptmp_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HGU4704356.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHKNP_RS10970"
                     /old_locus_tag="pgaptmp_002194"
     CDS             23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHKNP_RS10970"
                     /old_locus_tag="pgaptmp_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HGU4704357.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHKNP_RS10975"
                     /old_locus_tag="pgaptmp_002195"
     CDS             complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHKNP_RS10975"
                     /old_locus_tag="pgaptmp_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HGU4704358.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25029..26405
                     /gene="zraS"
                     /locus_tag="ACHKNP_RS10980"
                     /old_locus_tag="pgaptmp_002196"
     CDS             25029..26405
                     /gene="zraS"
                     /locus_tag="ACHKNP_RS10980"
                     /old_locus_tag="pgaptmp_002196"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HGU4704359.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26402..27727
                     /gene="zraR"
                     /locus_tag="ACHKNP_RS10985"
                     /old_locus_tag="pgaptmp_002197"
     CDS             26402..27727
                     /gene="zraR"
                     /locus_tag="ACHKNP_RS10985"
                     /old_locus_tag="pgaptmp_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HGU4704360.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCVALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTCGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHKNP_RS10990"
                     /old_locus_tag="pgaptmp_002198"
     CDS             complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHKNP_RS10990"
                     /old_locus_tag="pgaptmp_002198"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGU4704361.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHKNP_RS10995"
                     /old_locus_tag="pgaptmp_002199"
     CDS             complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHKNP_RS10995"
                     /old_locus_tag="pgaptmp_002199"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGU4704362.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7302 a         8042 c         8483 g         7170 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcat tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgacagacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gaggggggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaagtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaata ctgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt acgcgtcaga cagacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga ttccgggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtta ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac
    12781 tcataatcaa tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact
    12841 cggcaaggat tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa
    12901 gtggaaaagt ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt
    12961 gatgctcatc aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc
    13021 ttttgggtta cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa
    13081 gaaaaacaat cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt
    13141 tataacttag cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt
    13201 attcatgccg ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag
    13261 gcattttact ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta
    13321 actagcgctt atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt
    13381 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13441 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13501 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13561 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13621 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13681 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13741 gacggcacta ccataaaaaa acacaacgct ttcaacaacc ttgcgttttt catcttccgt
    13801 agcggctcgg aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcccgtc
    13861 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    13921 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat
    13981 cggcatagcc acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa
    14041 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14101 caagcagtcg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14161 gctcaatgcc gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc
    14221 agtaatgctg ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagt
    14281 tgtcggaaag gccaattagc gcgccgaggc ctatcttatc aatccccgca cgccccagcc
    14341 gatccggcgt ttccagccgc cagaagaagt cctgtttttt tcctttcaga tgatggcggg
    14401 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagctgct
    14461 ttaactcggc gtattccgtc tccgccagcg gttgcacttc catttgtagt gaagagaact
    14521 gttcacgcag ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14581 caccagtgac taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14641 caatatccgc ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14701 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    14761 tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac
    14821 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gagttgcgag gcatttagcg
    14881 cccgttctac gtcagcagcc gttttgccgt tgatacgcag gcggatgtcg tcccagtcca
    14941 gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cagtcagcgg
    15001 gctggtggca tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15061 tgcttctacc gccagacgaa atgccttcgc catgttgacg ggatcgtccg cgacggcaat
    15121 tgccgtattc actaacaccg cgtcggcccc catttccagc gcctgcgcgg catggctggg
    15181 aacgccgatg ccagcatcga caaccaccgg cactgtggcc tgctggataa taatctccag
    15241 catggcgcgg gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15301 tgcacagccg acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag
    15361 cacgacaaat ccctgttgta ccagcgtttc ggcggctttc agggtttcga tgggatcggg
    15421 caacagccag cgggcgtcag ggtgaatctc taattttaac cagtttgtgc ctaacgcttc
    15481 acgagccaga tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg
    15541 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15601 gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac
    15661 catcagttgt gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt
    15721 gtccgcaata cgtaacattt caaccccctg caataacctg aaaaagcagg atctggtcgc
    15781 catcctgcac gatatgttgc gcccactgct cacgcgggac gatttgctga ttaatcgcca
    15841 gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg
    15901 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    15961 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgatttacc gtcgaacagt
    16021 cggagttctc ccgcaggtgt ctctataccg cttaataact taatggcttc cagtgcctgc
    16081 aaagtgccca taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16141 ggctcctggt tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt
    16201 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc
    16261 acgcaggcgg cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16321 tcggcccgtg caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc
    16381 agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc tgacctgcga tttcgggcga
    16441 tcgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16501 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca ggtacagcgc agcaggtgta
    16561 cccagcccgc ccagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc
    16621 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc
    16681 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt
    16741 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc
    16801 cagactgata ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc
    16861 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    16921 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    16981 tgtcgaaacg cccagccgca ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa
    17041 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc
    17101 gttgataaac aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcggc
    17161 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17221 caacagacgt tcgatccact gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17281 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17341 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17401 gcgtaatcac gcacttcctg gctgattttc atcgagcaaa atttcggtcc acacatggag
    17461 caaaaatggg cgactttgcc ggactcttgg ggcagggttt catcgtgata agcgcgggcg
    17521 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17581 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17641 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17701 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    17761 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    17821 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    17881 tgcatcggca cgtggcctgg gccttcaatc atcacctgca cgtcatattc ccaggcaatt
    17941 ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18001 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18061 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18121 ttcgccataa tcgaaccgcc gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc
    18181 acatagcgca gcagtacgcc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18241 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18301 tccagcgcct ggtagatcgg cactgtaccg atcggcaccg ggctgttacg caaaatccac
    18361 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18421 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgcagagttg
    18481 ccgatattgg cgttaacttt taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc
    18541 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18601 tccgcagtga tattttccgg cagacgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18661 aaaacttcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18721 tccggtgtga tgatgccctg gcgggcgtag tgcagttggg tgacgcggcg tcctgctttg
    18781 gcgcgttttg gtgttagtac gccgctaaaa cgcagttcgt cgaggccatc atctgccagc
    18841 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    18901 atccacggct ggcgtagttt tgccagccct tgctgcacgt taatggcaat ctgcggatca
    18961 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19021 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19081 ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg
    19141 gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac
    19201 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19261 atcgccgcag gggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19321 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19381 attagccccg caaatggggc attgaatgta aattacgcgt taacagcgca gaactcaagc
    19441 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    19501 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    19561 atgatcgatg gcggtttcca gactggaatc gtagtaatcc ataatctgtt gagtattggc
    19621 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    19681 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    19741 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    19801 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    19861 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    19921 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    19981 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    20041 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    20101 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    20161 acctgtttgg ttagtacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    20221 tgatctcgat gttgggctgt ttcaactggc cggacgaggc gtacaactgg cggagtttta
    20281 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    20341 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    20401 tgttgctatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    20461 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    20521 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    20581 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    20641 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    20701 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg
    20761 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    20821 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    20881 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    20941 ccaggcaggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    21001 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    21061 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    21121 gccagtcacc tgcaaagctg acgtcgataa actgccaatt ccggacccgg aagatgaact
    21181 gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    21241 gattggtttt tccggcagcc cgtggacgct ggcgacctac atggtggaag gcggcagcag
    21301 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    21361 gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    21421 tcaggctgtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    21481 gttctcgctc tattacatgc ataaaattgt tgatggttta ctgcgtgaaa acgacggtcg
    21541 ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag tggctggaag cgatggcaga
    21601 aaccggttgc gatgcgctgg gcctcgactg gacaacggat atcgccgatg cgcgccgccg
    21661 cgtgggcaat aaagtcgcgt tgcagggcaa tatggatccg tcgatgctgt acgcgccgcc
    21721 agcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    21781 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    21841 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taaggagtga ttatggatct
    21901 cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct gtgatccgcg aggatcgact
    21961 cgataaagat ccaccggatc tgatcgccgg agccgatgtc gggtttgagc agggcggaga
    22021 agtgacgcga gcggcgatgg tgctgctgaa atatccctcg cttgagctgg tggagtataa
    22081 agtggcccgc atcgccacca ccatgcctta cattccaggt tttctttcct tccgcgaata
    22141 tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag ccggatttag tgtttgtcga
    22201 tggtcatggg atctcgcatc cccgccgtct tggcgtcgcc agccattttg gcttattggt
    22261 ggatgtgccg accattggcg tggcgaaaaa acggctctgc ggtaaattcg aaccgctctc
    22321 cagcgaaccg ggcgcgctgg ccccactgat ggataaaggc gagcagctgg cctgggtctg
    22381 gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc ggccatcggg tcagcgtgga
    22441 cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat cgtctgccgg agccaacgcg
    22501 ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc
    22561 ctaattcagg taaactgcgg ataatttccg tatttgagaa ctcaacatgt tacaaaaccc
    22621 aattcatctg cgtctggagc gcctggaaag ctggcagcat gtcactttca tggcttgctt
    22681 atgcgaacgc atgtacccca attacgccat gttctgccag caaaccggtt ttggtgatgg
    22741 gcaaatttac cgtcgcattc tcgatctcat ctgggaaacg ctgaccgtta aagacgcaaa
    22801 agtaaatttc gacagccaac tggagaaatt tgaagaagcg attccttcag ccgacgattt
    22861 cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca
    22921 ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac
    22981 gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa atgagcgatg aagagcttaa
    23041 agaaaaccca gctgtagagc aagaatggga cattcagtgg gaaatattcc gacttttagc
    23101 tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc
    23161 gggtgatagc aatattggta taatttttca gcaataagac cagaaaacgt gatttaacgc
    23221 ctgatttgtc gtacctggag tcttcccttt cgccccccgt ctggtctaca tttggggggc
    23281 gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc tattctggca tttccgtcgc
    23341 actcgatgct tagcaagcga taaacacatt gtaaggataa cttatgaaca agactcaact
    23401 gattgatgta attgcagaga aagcagaact gtccaaaacc caggctaaag ctgctctgga
    23461 gtccactctg gctgcaatta ctgagtctct gaaagaaggc gatgctgtac aactggttgg
    23521 tttcggtacc ttcaaagtga accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg
    23581 taaagaaatc aaaattgccg cagctaacgt accggcattt gtttctggca aggcactgaa
    23641 agacgcagtt aagtaagatt gcgtggcagt gaacagtttt aacgaagggg tggtttcacc
    23701 ccttttgtct ttctggcgtc gatcattgat gctggcaggt gcgctgtttc tcactgcctg
    23761 tagccacaac tcttcacttc ctccctttac cgccagtgga tttgctgaag accagggcgc
    23821 ggtacgcatc tggcgaaaag acagcggcga taatgtgcat ctgcttgccg tgtttagccc
    23881 gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg cagggcgata acctcacgct
    23941 catcaatatc aatgtttaca gcaaaccgcc ggtgaacatt cgcgcgcgtt ttgacgatcg
    24001 cggtgatctg agctttatgc aacgtgaatc cgatggggaa aagcagcagc tttctaacga
    24061 ccaaatcgat ttataccgtt atcgtgctgc tcagatccgc cagattagcg atgccttacg
    24121 tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg atggaacaga ccgtgaccac
    24181 ctgcgaaggg caaaccatta aacctgattt agattcgcag gcgatagcgc atatcgagcg
    24241 ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc
    24301 gcaattactg ttagtggcaa actctgattt ctgtcgctgg caacccaacg agaaaacgtt
    24361 ctgatttacc agtggcccat acccatatga ccgccaccac cgcagccgcc gtagcccatg
    24421 cccattccgg ctccgcgcgg aatacccgct tcagccatcg cgatatcccg tttcacccgt
    24481 aactcatcta acgactgacg caaattctcc atctctttgg cgaccgcgtt aattttgctg
    24541 ctatccggtg ggttcgcggc taacagggca ttgtattcat aacgcttcgt caccagttgc
    24601 tgttgcagtg cgctgctttg agcgtaaaag tcattatgga ttttctgcca cgctgtctgt
    24661 tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac cgtgtccgcc gtgagcaaat
    24721 gcagatgtcg atcccatcgc cattgctgaa agcgccatca ttaccagggc aattttcgtg
    24781 ttccgtttca tggttaatcc tccagtggtt gtcttacttc gggtattgca tcttccgtgc
    24841 caacgatgaa acgctgatat gacgggtaat ctggcatgat aaacgagtaa aaatgactcg
    24901 cctgctgcgg gtagcgagtc atttttactc attgaaactt aagcctttgt gttacagcgc
    24961 agggtaagcg ctgataaaag atggcatgat ttctgctgtc agaaagggat gagcaggcaa
    25021 agaagaagat gcgttttatg caacgttcta aagactcctt agctaaatgg ttaagcgcga
    25081 tcctccccgt ggtcattgtt gggctggtag ggttgtttgc ggtgacggtg attcgcgatt
    25141 atgggcgcga gactgccgcc gccagacaaa cgctgctgga aaaaggcagt gtacttattc
    25201 gcgctcttga atccggctcg cgcgtcggca tggggatgcg catgcatcat gcgcagcagc
    25261 aggccttact ggaagaaatg gccgggcagc ctggagtacg ttggtttgcg gtcacggatg
    25321 aacaaggaac aatcgtgatg catagcaact ccggcatggt gggaaaacag ctttattccc
    25381 cgcaggaaat gcagcagtta catccgggag atgaagaagc gtggcggcgg atcgatagcg
    25441 cagacggcga gcctgttctg gaaatttatc gccagtttca accgatgttt gctgctggaa
    25501 tgcaccggat gcgccatatg cagcagtatg ccgcgacacc acaagcaatt ttcatcgctt
    25561 tcgacgccag taatattgtg agtgccgaag atcgtgagca gagaaacacc ctgattatcc
    25621 tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc attcttctgg tatcgccgct
    25681 atctgcgctc gcgccagctt ctacaagatg aaatgaagcg caaagagaag ctagtggcgc
    25741 tggggcatct tgcggcaggc gttgcccacg aaatccgtaa cccactttcc tcgattaaag
    25801 gactggcgaa atactttgcc gagcgcgcgc ctgcaggggg agaagcgcat caactggcgc
    25861 aggtgatggc gaaagaggcc gaccgtttaa accgcgtggt aagcgagctg ctggaactgg
    25921 ttaagccaac gcatctggct ttgcaggcgg tggatctcaa cacgctgatt aaccactcat
    25981 tacagctggt aggccaggat gcaaacagcc gggagatcca gttacgcttt accgccaacg
    26041 acacattacc ggaaattcag gccgatccgg acaggctgac tcaggtcctg ttgaatctct
    26101 atctcaatgc tattcaggcg attggtcagc atggcgtgat tagcgtgacg gtcagcgaaa
    26161 gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa gggaattgcg gcagatcagc
    26221 ttgaagccat cttcactccg tacttcacca ccaaagccga aggcaccgga ttggggctgg
    26281 cggtcgtgca taatattgtt gaacaacacg gtggtacaat tcaggtcgca agccaggagg
    26341 gaaaaggctc aacgttcacc ctctggcttc cggtcaatat tacgcgtaag gacccacaag
    26401 gatgacgcac gataatatcg atattctggt ggtggatgat gacattagcc actgcactat
    26461 tttgcaggct ttactgcgcg gctggggcta taacgtcgcg ctggcgaaca gcgggcgaca
    26521 ggcgctggag caggtgcggg agcgggtttt tgatcttgtg ctttgcgatg tgcgaatggc
    26581 agagatggac ggcattgcca cgctaaaaga gatcaaagcg ttaaatccgg cgattccggt
    26641 actgattatg accgcatact ccagcgtcga gacggcggta gaggcactga aaaccggggc
    26701 gctggattat ctcatcaagc cgctggattt cgacaccctg caggcgacgc tggaaaaggc
    26761 gctcgcgcat acgcacagta ttgatgctga aacgcctgcg gtgactgcca gccagttcgg
    26821 tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt gaaatcgccc tcgtcgcgcc
    26881 atcggaagcc acggtgctga tccacggcga ttccggcacc ggtaaagagc tggtcgccag
    26941 ggcgattcac gccagtagcg cacgtagcga aaaaccgctg gtaacgctca actgtgtggc
    27001 actcaacgaa tccttgctgg aatctgaatt gttcggtcac gaaaaagggg cgtttactgg
    27061 agccgataaa cgccgggaag ggcgctttgt tgaggcggac ggcggcacgc tgtttctcga
    27121 tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg ctgcgtgcga ttcaggagcg
    27181 cgaagttcag cgtgtcggta gcaaccagac tatctcggtt gatgtccggc tgattgcggc
    27241 gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt tttcgccagg atctctacta
    27301 tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg caacggcggg aggatatccc
    27361 tctgctggct agccattttc ttcagcgttt tgccgagcgt aatcgcaagg cggtaaaagg
    27421 ctttacgcca caggcgatgg atttgctgat ccactacgac tggccgggaa acattcgtga
    27481 actggaaaac gcggtggaaa ggtcggtggt gctgctgacc ggggaatata tttccgaacg
    27541 cgagctgccg ctggcgattg ccagtacgcc gatcccgctg gggcaaagtc aggatattca
    27601 gccgctggtg gaagtcgaaa aagaagtgat tctggcggca ctggagaaaa cgtgcggcaa
    27661 caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa acgctattgg caaaactgtc
    27721 gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata tctttccggc agaagcagtc
    27781 gtcccagtga atatcggtca ttaaggcata agcgcgtttc tgcgcttctg ccacggtatg
    27841 acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc actacctgct cgttatccgc
    27901 cagtttcgtg cccgcgtgga acactttgcc gcctgccact tcttccagcg gcaggccgtg
    27961 gatcacgtca ccggtgcggt aatcacccgg atatccaccc gcagccatca ccacgccgag
    28021 agaagcgcgt tcatcccact ctgacgtttt ctcgtccagt ttgccttcac aggccgccag
    28081 gcagagctca accagatcgg acttcatgcg cagcataatc ggctgggttt ctggatcgcc
    28141 aaagcggcag ttaaattcga taaccttcgg attgccctgt ttgtcgatca tcaggcccgc
    28201 gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg cctttcacgg ttggccagat
    28261 gatacgttcc atggtgcgct gatgaacttc atcggttact accggcgcgg gggagtaagc
    28321 gcccatcccg cccgtgtttg gtccggtatc tttatcgcct acgcgtttgt gatcctggct
    28381 ggtggccatc ggcagcacat gctcgccgtc taccatcacg ataaagctcg cttcttcgcc
    28441 atcgaggaac tcttcgataa cgatgcgatg acccgcgtcg ccaaaagcgt tgcccgccag
    28501 catatcgtga acagccgctt ccgcttcttc cagcgtcatc gccacgataa cgcctttccc
    28561 ggcagccaga ccgtccgctt taatgacgat tggcgcgcct ttctcacgca gatacgccag
    28621 cgcaggttct acctcggtga agttctggta ttccgccgta gggatcttat ggcgggccag
    28681 gaaatctttg gtaaacgctt ttgagccttc cagttgggcc gcacctgcgg ttgggccgaa
    28741 gattttcagc ccggcggcgc ggaaggtatc gaccacgcct ttcaccagcg gcgcttccgg
    28801 gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa tccaacagcg ccgggatatc
    28861 ggtcacgcca atagcaacgt tttgcagcgc gggttccagt gcagtgcctg cattacccgg
    28921 agcaacaaaa acagtctcaa ccagcggcga ctgggccgct ttccaggcca gcgcgtgctc
    28981 gcgcccgccg ttaccaataa ctaatacttt catctattgc tccattaatg gcggaagtgg
    29041 cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg cggcggcaat cacttcgtca
    29101 tcacggatgg aaccgccagg ctggattacg caggtcacgc ccgcagcggc ggcggcatca
    29161 ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg aggaaccttt cacttccagg
    29221 ccttcatcgg cagctttaat accggcgatt ttcgcggagt acacgcggct catctggccc
    29281 gcgccaatac cgatggtcat attgtttttg gcatagacga tagcattgga tttcacgaac
    29341 ttcgccactt tccagcagaa cagcgcatcg cgcagttcct gttcggtcgg ctgacgttgg
    29401 gtgacgacgc gcagttcttc cgcaccgacc atccccaggt cacgatcctg aaccagcaga
    29461 ccgccgttca cgcgtttgaa atcaagaccc ggaacacgct cgccccactg accgcaggtc
    29521 agaacgcgta cgttctgctt ggcggcggtg attttcaggg cttcttcgct ggcggaaggc
    29581 gcaataatca cttcgacaaa ctgacgagaa atgatggcct gtgcggtttc cgcatctagc
    29641 tcgcggttaa aggcaatgat gccgccgaat gcggaggttg ggtcggtttt gtacgcgcga
    29701 tcgtaagcat caagaatgga attgccgata gctacaccgc aagggttggc gtgcttcaca
    29761 atcacacatg ccggctcggc gaactctttc acgcactcca gcgccgcatc ggtatcggcg
    29821 atgttgttat aagagagggc tttaccctga acctgggttg cggtagcaac ggaggcttct
    29881 ttcacattct cttctatata gaaggcagcc tgctggtggc tgttctcgcc gtaacgcata
    29941 tcctgcttct taatgaagtt caggttcagc gtgcgtggga agcgaccagc ggcttctttg
    30001 ctttcaccgt ggtacgccgg aaccatgctg ccgaagtagt tggcaatcat gctgtcgtag
    30061 gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac gggttgcaag cgtcagcgat
    30121 ccttcgttgt catccatctc tttaataatg gcgtcgtagt cgctgctctt caccacgatt
    30181 gcgacatctt tatggttctt agcggcggag cgcaccatgg tcgggccgcc gatatcgatg
    30241 ttctcaaccg catcttccag cgagcaacct tcacgggcca cggtctgggc gaacggatac
    30301 aggttaacaa ccaccatatc gataggctgg atctgatgtt cttccataat ggcatcgtcc
    30361 tggccgcgac ggcccagaat gccaccatgt actttcggat gcagggtctt cacgcgtcca
    30421 tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg ttaccggcag acctttttct
    30481 gctaacagac gggcagtgcc ccctgtagac agcagctcca caccgcgtgc ggaaagtgcc
    30541 tgggcgaatt cgacgatacc ggctttgtca gaaacactga gcagagcgcg gcggactgga
    30601 cgacgttgtt gcatggtaaa tcccctggat ttgactatta cagagagcgt tagctgaatt
    30661 tttcgcgaaa aactcagcta acgcccctaa cggggcatcc ttatttttcg cccacattgt
    30721 aacgaaaacg tttgcgcaac gctcgcgaat ttttctcttt caatggtgat cacaattttg
    30781 acagtggtta ccgtgggaaa aatacagaaa ttacattgat gattgtggat aactctgtgc
    30841 gtaaaaaggt ataaagcggg gttttgctgg ggaatgcagc agtcagtcat ttttctgcaa
    30901 tttttctatt gcggcctgcg gagaactccc tataatgcgc ctccatcgac acggcggatg
    30961 tgaatcactt cacacaaaca gccggttcgg ttgaaga
//
LOCUS       DAZFWL020000045        30209 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFWL020000045 DAZFWL020000000
VERSION     DAZFWL020000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.045"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHKNP_RS11000"
                     /old_locus_tag="pgaptmp_002200"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHKNP_RS11000"
                     /old_locus_tag="pgaptmp_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HGU4704363.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHKNP_RS11005"
                     /old_locus_tag="pgaptmp_002201"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHKNP_RS11005"
                     /old_locus_tag="pgaptmp_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU4704364.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHKNP_RS11010"
                     /old_locus_tag="pgaptmp_002202"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHKNP_RS11010"
                     /old_locus_tag="pgaptmp_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HGU4704365.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLSVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="ACHKNP_RS11015"
                     /old_locus_tag="pgaptmp_002203"
     CDS             complement(1087..1581)
                     /locus_tag="ACHKNP_RS11015"
                     /old_locus_tag="pgaptmp_002203"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HGU4704366.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHKNP_RS11020"
                     /old_locus_tag="pgaptmp_002204"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHKNP_RS11020"
                     /old_locus_tag="pgaptmp_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HGU4704367.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHKNP_RS11025"
                     /old_locus_tag="pgaptmp_002205"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHKNP_RS11025"
                     /old_locus_tag="pgaptmp_002205"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HGU4704368.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHKNP_RS11030"
                     /old_locus_tag="pgaptmp_002206"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHKNP_RS11030"
                     /old_locus_tag="pgaptmp_002206"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HGU4704369.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHKNP_RS11035"
                     /old_locus_tag="pgaptmp_002207"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHKNP_RS11035"
                     /old_locus_tag="pgaptmp_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HGU4704370.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHKNP_RS11040"
                     /old_locus_tag="pgaptmp_002208"
     CDS             complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHKNP_RS11040"
                     /old_locus_tag="pgaptmp_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HGU4704371.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHKNP_RS11045"
                     /old_locus_tag="pgaptmp_002209"
     CDS             complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHKNP_RS11045"
                     /old_locus_tag="pgaptmp_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HGU4704372.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="ACHKNP_RS11050"
                     /old_locus_tag="pgaptmp_002210"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="ACHKNP_RS11050"
                     /old_locus_tag="pgaptmp_002210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HGU4704373.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="ACHKNP_RS11055"
                     /old_locus_tag="pgaptmp_002211"
     CDS             complement(9275..9769)
                     /locus_tag="ACHKNP_RS11055"
                     /old_locus_tag="pgaptmp_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HGU4704374.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIINVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="ACHKNP_RS11060"
                     /old_locus_tag="pgaptmp_002212"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="ACHKNP_RS11060"
                     /old_locus_tag="pgaptmp_002212"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU4704375.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHKNP_RS11065"
                     /old_locus_tag="pgaptmp_002213"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHKNP_RS11065"
                     /old_locus_tag="pgaptmp_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HGU4704376.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHKNP_RS11070"
                     /old_locus_tag="pgaptmp_002214"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHKNP_RS11070"
                     /old_locus_tag="pgaptmp_002214"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU4704377.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="ACHKNP_RS11075"
                     /old_locus_tag="pgaptmp_002215"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="ACHKNP_RS11075"
                     /old_locus_tag="pgaptmp_002215"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGU4704378.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /gene="yghB"
                     /locus_tag="ACHKNP_RS11080"
                     /old_locus_tag="pgaptmp_002216"
     CDS             13861..14520
                     /gene="yghB"
                     /locus_tag="ACHKNP_RS11080"
                     /old_locus_tag="pgaptmp_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HGU4704379.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHKNP_RS11085"
                     /old_locus_tag="pgaptmp_002217"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHKNP_RS11085"
                     /old_locus_tag="pgaptmp_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HGU4704380.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHKNP_RS11090"
                     /old_locus_tag="pgaptmp_002218"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHKNP_RS11090"
                     /old_locus_tag="pgaptmp_002218"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HGU4704381.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHKNP_RS11095"
                     /old_locus_tag="pgaptmp_002219"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHKNP_RS11095"
                     /old_locus_tag="pgaptmp_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HGU4704382.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17948..18874
                     /locus_tag="ACHKNP_RS11100"
                     /old_locus_tag="pgaptmp_002220"
     CDS             17948..18874
                     /locus_tag="ACHKNP_RS11100"
                     /old_locus_tag="pgaptmp_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HGU4704383.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHKNP_RS11105"
                     /old_locus_tag="pgaptmp_002221"
     CDS             18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHKNP_RS11105"
                     /old_locus_tag="pgaptmp_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HGU4704384.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19225..21444)
                     /locus_tag="ACHKNP_RS11110"
                     /old_locus_tag="pgaptmp_002222"
     CDS             complement(19225..21444)
                     /locus_tag="ACHKNP_RS11110"
                     /old_locus_tag="pgaptmp_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HGU4704385.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHKNP_RS11115"
                     /old_locus_tag="pgaptmp_002223"
     CDS             complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHKNP_RS11115"
                     /old_locus_tag="pgaptmp_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HGU4704386.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHKNP_RS11120"
                     /old_locus_tag="pgaptmp_002224"
                     /gene_synonym="parF"
     CDS             complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHKNP_RS11120"
                     /old_locus_tag="pgaptmp_002224"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HGU4704387.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHKNP_RS11125"
                     /old_locus_tag="pgaptmp_002225"
     CDS             complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHKNP_RS11125"
                     /old_locus_tag="pgaptmp_002225"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGU4704388.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLEQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26816..27298)
                     /locus_tag="ACHKNP_RS11130"
                     /old_locus_tag="pgaptmp_002226"
     CDS             complement(26816..27298)
                     /locus_tag="ACHKNP_RS11130"
                     /old_locus_tag="pgaptmp_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGU4704389.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPIMFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHKNP_RS11135"
                     /old_locus_tag="pgaptmp_002227"
     CDS             complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHKNP_RS11135"
                     /old_locus_tag="pgaptmp_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HGU4704390.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27895..28554
                     /gene="qseB"
                     /locus_tag="ACHKNP_RS11140"
                     /old_locus_tag="pgaptmp_002228"
     CDS             27895..28554
                     /gene="qseB"
                     /locus_tag="ACHKNP_RS11140"
                     /old_locus_tag="pgaptmp_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HGU4704391.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28551..29900
                     /gene="qseC"
                     /locus_tag="ACHKNP_RS11145"
                     /old_locus_tag="pgaptmp_002229"
     CDS             28551..29900
                     /gene="qseC"
                     /locus_tag="ACHKNP_RS11145"
                     /old_locus_tag="pgaptmp_002229"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HGU4704392.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29946..>30209)
                     /locus_tag="ACHKNP_RS11150"
                     /old_locus_tag="pgaptmp_002230"
     CDS             complement(29946..>30209)
                     /locus_tag="ACHKNP_RS11150"
                     /old_locus_tag="pgaptmp_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HGU4704393.1"
                     /translation="DAFFAFNKYDWMLESGDNICSIPSVSGEDRILQAMIAAFFLLTP
                     LIILILRKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY"
BASE COUNT         7455 a         8076 c         7631 g         7047 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagtag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcgacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca agcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagag
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tgacagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catgctgtgc gaaagcggcg acatcagcga acaggagaga
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tagtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgccagatt cacgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcacc
     1261 ggtgaagcgc agggccgaca atacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgtgttctt
     1381 tttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt aatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggtg
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc
     2041 acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacc accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt gaccccattg
     3901 aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac atcaccttgt
     3961 tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccccccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtaccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgcta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagctggtt
     9061 gttgaaagat gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg agcaactaga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg ttaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcacaa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc ctatctggac ggcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagaaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcggcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac atgttgcaat agatattgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 atccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aagtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaatgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tacgcagtgt ggtgccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttctattca agccgccacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tacgggaaaa cgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc aataatgaag
    13501 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggcg aatcaaccag aacatatcgc cgccattcgc ccccaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac ttttggacaa
    13741 ttttgcagac atttaattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagt
    14341 ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgtaca tcgggcaaca gttctgacaa actattttct ttaattttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggaggaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctgatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg gcggcgacgc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgatg atgagcgtat
    16621 tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgttg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    17881 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    17941 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    18001 acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga actataaatc
    18061 agatgccact gccccttatt ttggtgaaac cggagagcgg gcgataactt ctgcgcgtat
    18121 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    18181 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgttgcgat
    18241 aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    18301 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    18361 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    18421 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    18481 tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    18541 ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat
    18601 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt
    18661 ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gtattcatgt
    18721 tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac agcggcgcga
    18781 aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    18841 tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga
    18901 acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg
    18961 gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct ggcgagcaat
    19021 gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca
    19081 caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgctcaggac
    19141 tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag
    19201 catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc
    19261 accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg
    19321 acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc ggttctggcg
    19381 acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc
    19441 aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg
    19501 atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa
    19561 gacgtcttcg ctcttataac caatcttcgc cagcgggttt ttcccggtgt aatacatggt
    19621 ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg
    19681 ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg ggtgcgcgga
    19741 gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag tatcgaacag
    19801 ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata acggcccttc
    19861 ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt
    19921 gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga tgaggatttt
    19981 tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt
    20041 gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc
    20101 gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat
    20161 tacgcccgta aaaccaggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc
    20221 ttccgaacgg ctctgaataa tgcgcccttc gtgctcggtg atagaacaga aagagcagcc
    20281 gccaaagcag ccgcgcataa tgttgaccga gaaacggatc atttcgtaag ccggaatacg
    20341 ggcattgcca taggccggat gcggcacgcg cttgtacggc agcgcaaaaa cgctgtccat
    20401 ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt
    20461 ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg
    20521 agcgtacagg actttatcgc ccttcacttt ctcgaaagaa ggcagcaata cgtaggtttt
    20581 ttcccacggt ttcgggcgcg gcggctgcac ggttacagct ttcgcttcct gctttttcgg
    20641 cgcaacaggt ttgttatccg cgcacggcag atcttcgcca tacggatgcg ggattgggtc
    20701 gattttccct ggggtatcaa gacgagtaga atccacaccg ctccagccag gcagcgcctc
    20761 ttttacgata attgcggtat tacgcacatc gcggatttcg ctaattggct cgcccatcgc
    20821 cagacgatgc gctacttcca ccagcggacg ttcaccgtta ccaaacatca gcatgtcggc
    20881 tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat aatgcgcggt
    20941 acggcgcaga ctagcctcaa taccgccaag gatcaccggc acatctttcc acgcctcttt
    21001 acaacgctgg gtataaacca gtgtggcgcg atccggtcgc ttacccgcga cgttatccgg
    21061 tgtgtaggca tcgtcatgac gtaaacggcg atcggcggta tagcggttga tcatcgaatc
    21121 catgttgcca gcagtaacac caaaaaacag attcggttta cccagacgca taaagtcgtc
    21181 tttgctgctc cagtccggct gggcgatgat cccgacgcga aagccctgcg cttccagcat
    21241 acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa
    21301 aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa
    21361 cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg
    21421 ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc
    21481 cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc gccctcctcg
    21541 ggcgagtatg aagattacgg taccggattg accaacagtt gcccaatcga gccacggtcc
    21601 gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg
    21661 aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct
    21721 tccgggaacg gcatcgcgcc attcacgtta cggatctgga acatcacgcc ttcaatatgg
    21781 aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg
    21841 acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc acccagacta
    21901 atatcgcgac tgcgaatcgg cgaaccggcc atgatttcag ttggcagcaa gcgcatcgga
    21961 agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac cagggtagaa
    22021 accaaaatgc tggatggctc aaagaagcca cgaatacgat caacaatgct cgccgcttcg
    22081 ccacaggtga tcgacacttc atcaccgttg ctcatatcca ccagaatctc gcggcgctcg
    22141 cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc ctgatcgcca
    22201 gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg agagttcgac
    22261 gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg gctttgtaca
    22321 ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt gtattctggc
    22381 gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc cacaccataa
    22441 tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat tccggcaagg
    22501 ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct
    22561 gcgttctggc gaatgggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc
    22621 ggaccgccca tcagcggacc tggtacctgt agcccggcca ccgtcattga gacattttct
    22681 gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc
    22741 cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt aaatgaccag
    22801 tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag tagcggcgga
    22861 acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa
    22921 agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt
    22981 aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac agattgccga
    23041 cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt
    23101 gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg ccatactgac
    23161 tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt cgattaagat
    23221 taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc gccgcaattg
    23281 ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg cgggttcctt
    23341 ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact tccgcaatgg
    23401 tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccagtaca
    23461 actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc gtcggcggtt
    23521 gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg atatagatag
    23581 cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg ccgaacagcg
    23641 gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta cgcgggctga
    23701 aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg gtaataataa
    23761 gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac
    23821 ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc
    23881 aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc
    23941 tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg aaacaggccc
    24001 tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga gtcgatctca
    24061 acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg ttcgccagtg
    24121 actttctgta actcttccgg gcgcagttta attttgcgtt tcccaacatg aatggtcagc
    24181 gtgctttgcg gcggcagaac gtacaattgc gccagaccat cttctccacg cgcggcttct
    24241 gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg cagatcgctt
    24301 accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc ggaagcatct
    24361 tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa agccttacct
    24421 gcacggttac gcgccaccag atcgttaaag gtgcagacga aaccgtaacc cgcatcggaa
    24481 gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt cgccccaggc
    24541 ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg cagcgtaatc
    24601 gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg gttgctctta
    24661 cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg cgcgtcgata
    24721 tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt gacaggttca
    24781 gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg caacggcgaa
    24841 cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag caggttattc
    24901 attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc cagctcactc
    24961 tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt cagttcgagg
    25021 atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg tttcggttca
    25081 tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac cagcaaacct
    25141 tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg gcggcgcacg
    25201 gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt caccgccgga
    25261 cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag atcggtggta
    25321 gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg cacaatcacc
    25381 agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat cggcagcttt
    25441 ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac ctgatgcggt
    25501 aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat acgcactgaa
    25561 ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg aagtgataat ttccgcttca
    25621 gtcggataat ccggcccctg cacgatatcc agcagctgct cgagcgtggt tttcggctgg
    25681 tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg aatatcggtc
    25741 gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag acgagcaggc
    25801 agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc cgtcccctgc
    25861 cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt gtaacgcatt
    25921 gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc aaccagcgga
    25981 taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc gctatcgccg
    26041 tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt aaatttggcg
    26101 ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac aggtttaaga
    26161 ccatcgccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta gtttaagtag
    26221 gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat taattctgat
    26281 tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact acattatctg
    26341 ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc cgacggcgat
    26401 aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg gcatagaggg
    26461 cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc gggcgattcg
    26521 agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc gactgtaagt
    26581 atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc tgccataagt
    26641 gcggcttgct catggatacc cccgagacgg cttacggcgc accggataat aaatttcgcg
    26701 tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc ctgtggcatt
    26761 gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt ccccatcacg
    26821 ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg gcaagattgg
    26881 tgtagtgaaa cataatcggt gctttacgca ttttctcgcc gcttgcaggc agccagtggc
    26941 gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg cgggccacgg
    27001 cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg ggaatcggtt
    27061 cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg gcagggtcgt
    27121 cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc tctttacgcc
    27181 acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg tgcggcaaca
    27241 tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg tttgtcattt
    27301 tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa ttacggattt
    27361 actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc gacttcaccc
    27421 tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt gtggtcgata
    27481 tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga gatgcgttca
    27541 acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc gctttctacg
    27601 gtcgttacgc tgccgttcgg cccctggaat cctccggcct gactttgcgt tgccgatggg
    27661 ccagaaaaac cgccctgctc tgctgccatc accggtgcgc tgcacagggc cattactgcg
    27721 attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt ccgagcattt
    27781 aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttagtg ctgtacagag
    27841 cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga aaaaatgcga
    27901 attttactga tagaagatga catgctgatt ggcgacggca tcaaaacggg ccttagtaaa
    27961 atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc gctttatagc
    28021 gcgccttatg atgcggtgat cctggattta accttaccag gcatggatgg tcgcgatatt
    28081 ttgcgcgaat ggcgagaaaa aggtcagcgt gagccggtac tgatcctgac cgcgcgcgat
    28141 gcgctggagg aacgtgtaga agggctgcgt ctgggagctg acgattatct gtgtaaacct
    28201 tttgcgttga tagaagtcgc cgccaggctg gaagctctga tgcgccgaac caacggccag
    28261 gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg tatcgccacg
    28321 ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga attactgatg
    28381 cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta tacctgggac
    28441 gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg caaactcggt
    28501 agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa atgaaattta
    28561 cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg gcctcggtga
    28621 cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc gatgaattgt
    28681 tcgacaccca actgatgctg tttgccaagc ggttaagtac gctcgatctc aacgaaatca
    28741 acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat gttgatgacg
    28801 atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaac gatggcgata
    28861 acggagaaga tattccttat agctatcaac gggaaggttt tgctgacggg caactggtgg
    28921 gcgataaaga tcaatggcgt tttgtctgga tgacctcacc tgatggcaaa tatcgcatcg
    28981 ttgttggtca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt gccgggcaat
    29041 tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta ctattgggtc
    29101 gtgaactcgc gccgctgaac aaactggcgc tggcgctgcg tatgcgtgac cctgactcgg
    29161 aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg tccgttggtt gagtcgctaa
    29221 atcaactgtt cgcccgcaca catgcgatga tggttcgtga acgacgcttt acctccgacg
    29281 cagctcacga actacgtagc ccgttaacgg cgctgaaagt gcaaaccgaa gttgcgcagc
    29341 tttctgacga tgatccgcaa gcgcggaaaa aagcgctgct tcaattacat tacgggatcg
    29401 atcgcgctac tcgtctggtt gatcaactgc tcactctatc gcggctggac tcactggata
    29461 accttcagga cgtcgcggag atcccgcttg aagatctcct gcaatcgtcg gtgatggata
    29521 tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat gcccacggca
    29581 tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg ctggataacg
    29641 ccgtgcgcta cagtccacag ggcagcgtga tagacgtcac gctgaatgct gataatttca
    29701 tcgtgaggga taacggcccc ggtgtgacgc cagaggctct ggcgcgaatt ggcgaacgct
    29761 tctatcgccc ccccggacaa accgctaccg gcagtggact tgggctatcg attgtccagc
    29821 gaatcgccaa attgcatggc atgaatgttg agtttgggaa tgcggaacaa ggtggatttg
    29881 aggcgaaggt aagctggtaa cttctctgtc tatgagagcc gttaaaacga ctctcataga
    29941 ttttactaat agcaaaatat aaaccgtccc caaaaaagcc accaaccaca aaccagacaa
    30001 ataccgagcg agaatacata tacccaaaac tcgaacattt cccgcataaa gagttttctt
    30061 aagataagaa taataagtgg cgtaagaaga aaaaatgctg caatcattgc ttgcaatata
    30121 cgatcttcgc ctgaaacgga aggtatactg caaatattgt ctcccgactc aagcatccag
    30181 tcatacttat tgaaagcaaa aaaagcatc
//
LOCUS       DAZFWL020000046        29712 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFWL020000046 DAZFWL020000000
VERSION     DAZFWL020000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.046"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(114..1943)
                     /gene="glmS"
                     /locus_tag="ACHKNP_RS11155"
                     /old_locus_tag="pgaptmp_002231"
     CDS             complement(114..1943)
                     /gene="glmS"
                     /locus_tag="ACHKNP_RS11155"
                     /old_locus_tag="pgaptmp_002231"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGU4704394.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2105..3475)
                     /gene="glmU"
                     /locus_tag="ACHKNP_RS11160"
                     /old_locus_tag="pgaptmp_002232"
     CDS             complement(2105..3475)
                     /gene="glmU"
                     /locus_tag="ACHKNP_RS11160"
                     /old_locus_tag="pgaptmp_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGU4704395.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3826..4245)
                     /gene="atpC"
                     /locus_tag="ACHKNP_RS11165"
                     /old_locus_tag="pgaptmp_002233"
     CDS             complement(3826..4245)
                     /gene="atpC"
                     /locus_tag="ACHKNP_RS11165"
                     /old_locus_tag="pgaptmp_002233"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGU4704396.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4266..5648)
                     /gene="atpD"
                     /locus_tag="ACHKNP_RS11170"
                     /old_locus_tag="pgaptmp_002234"
     CDS             complement(4266..5648)
                     /gene="atpD"
                     /locus_tag="ACHKNP_RS11170"
                     /old_locus_tag="pgaptmp_002234"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGU4704397.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5675..6538)
                     /gene="atpG"
                     /locus_tag="ACHKNP_RS11175"
                     /old_locus_tag="pgaptmp_002235"
     CDS             complement(5675..6538)
                     /gene="atpG"
                     /locus_tag="ACHKNP_RS11175"
                     /old_locus_tag="pgaptmp_002235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGU4704398.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YADTMRKVIGHLASGNLEYKHPYLEERDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6589..8130)
                     /gene="atpA"
                     /locus_tag="ACHKNP_RS11180"
                     /old_locus_tag="pgaptmp_002236"
     CDS             complement(6589..8130)
                     /gene="atpA"
                     /locus_tag="ACHKNP_RS11180"
                     /old_locus_tag="pgaptmp_002236"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGU4704399.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLVDVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8143..8676)
                     /gene="atpH"
                     /locus_tag="ACHKNP_RS11185"
                     /old_locus_tag="pgaptmp_002237"
     CDS             complement(8143..8676)
                     /gene="atpH"
                     /locus_tag="ACHKNP_RS11185"
                     /old_locus_tag="pgaptmp_002237"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGU4704400.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8691..9161)
                     /gene="atpF"
                     /locus_tag="ACHKNP_RS11190"
                     /old_locus_tag="pgaptmp_002238"
     CDS             complement(8691..9161)
                     /gene="atpF"
                     /locus_tag="ACHKNP_RS11190"
                     /old_locus_tag="pgaptmp_002238"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGU4704401.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9223..9462)
                     /gene="atpE"
                     /locus_tag="ACHKNP_RS11195"
                     /old_locus_tag="pgaptmp_002239"
     CDS             complement(9223..9462)
                     /gene="atpE"
                     /locus_tag="ACHKNP_RS11195"
                     /old_locus_tag="pgaptmp_002239"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGU4704402.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9509..10324)
                     /gene="atpB"
                     /locus_tag="ACHKNP_RS11200"
                     /old_locus_tag="pgaptmp_002240"
     CDS             complement(9509..10324)
                     /gene="atpB"
                     /locus_tag="ACHKNP_RS11200"
                     /old_locus_tag="pgaptmp_002240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGU4704403.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10333..10713)
                     /gene="atpI"
                     /locus_tag="ACHKNP_RS11205"
                     /old_locus_tag="pgaptmp_002241"
     CDS             complement(10333..10713)
                     /gene="atpI"
                     /locus_tag="ACHKNP_RS11205"
                     /old_locus_tag="pgaptmp_002241"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HGU4704404.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11330..11953)
                     /gene="rsmG"
                     /locus_tag="ACHKNP_RS11210"
                     /old_locus_tag="pgaptmp_002242"
     CDS             complement(11330..11953)
                     /gene="rsmG"
                     /locus_tag="ACHKNP_RS11210"
                     /old_locus_tag="pgaptmp_002242"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGU4704405.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGH
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(12017..13906)
                     /gene="mnmG"
                     /locus_tag="ACHKNP_RS11215"
                     /old_locus_tag="pgaptmp_002243"
     CDS             complement(12017..13906)
                     /gene="mnmG"
                     /locus_tag="ACHKNP_RS11215"
                     /old_locus_tag="pgaptmp_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGU4704406.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14284..14727)
                     /gene="mioC"
                     /locus_tag="ACHKNP_RS11220"
                     /old_locus_tag="pgaptmp_002244"
     CDS             complement(14284..14727)
                     /gene="mioC"
                     /locus_tag="ACHKNP_RS11220"
                     /old_locus_tag="pgaptmp_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HGU4704407.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14817..15275)
                     /gene="asnC"
                     /locus_tag="ACHKNP_RS11225"
                     /old_locus_tag="pgaptmp_002245"
     CDS             complement(14817..15275)
                     /gene="asnC"
                     /locus_tag="ACHKNP_RS11225"
                     /old_locus_tag="pgaptmp_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HGU4704408.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15427..16419
                     /gene="asnA"
                     /locus_tag="ACHKNP_RS11230"
                     /old_locus_tag="pgaptmp_002246"
     CDS             15427..16419
                     /gene="asnA"
                     /locus_tag="ACHKNP_RS11230"
                     /old_locus_tag="pgaptmp_002246"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGU4704409.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16424..17875)
                     /gene="viaA"
                     /locus_tag="ACHKNP_RS11235"
                     /old_locus_tag="pgaptmp_002247"
     CDS             complement(16424..17875)
                     /gene="viaA"
                     /locus_tag="ACHKNP_RS11235"
                     /old_locus_tag="pgaptmp_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HGU4704410.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17869..19365)
                     /gene="ravA"
                     /locus_tag="ACHKNP_RS11240"
                     /old_locus_tag="pgaptmp_002248"
     CDS             complement(17869..19365)
                     /gene="ravA"
                     /locus_tag="ACHKNP_RS11240"
                     /old_locus_tag="pgaptmp_002248"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HGU4704411.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19588..21456
                     /gene="kup"
                     /locus_tag="ACHKNP_RS11245"
                     /old_locus_tag="pgaptmp_002249"
     CDS             19588..21456
                     /gene="kup"
                     /locus_tag="ACHKNP_RS11245"
                     /old_locus_tag="pgaptmp_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HGU4704412.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDTVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21623..22042
                     /gene="rbsD"
                     /locus_tag="ACHKNP_RS11250"
                     /old_locus_tag="pgaptmp_002250"
     CDS             21623..22042
                     /gene="rbsD"
                     /locus_tag="ACHKNP_RS11250"
                     /old_locus_tag="pgaptmp_002250"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HGU4704413.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            22050..23555
                     /gene="rbsA"
                     /locus_tag="ACHKNP_RS11255"
                     /old_locus_tag="pgaptmp_002251"
     CDS             22050..23555
                     /gene="rbsA"
                     /locus_tag="ACHKNP_RS11255"
                     /old_locus_tag="pgaptmp_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HGU4704414.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23560..24525
                     /gene="rbsC"
                     /locus_tag="ACHKNP_RS11260"
                     /old_locus_tag="pgaptmp_002252"
     CDS             23560..24525
                     /gene="rbsC"
                     /locus_tag="ACHKNP_RS11260"
                     /old_locus_tag="pgaptmp_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGU4704415.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24550..25440
                     /gene="rbsB"
                     /locus_tag="ACHKNP_RS11265"
                     /old_locus_tag="pgaptmp_002253"
     CDS             24550..25440
                     /gene="rbsB"
                     /locus_tag="ACHKNP_RS11265"
                     /old_locus_tag="pgaptmp_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HGU4704416.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25566..26495
                     /gene="rbsK"
                     /locus_tag="ACHKNP_RS11270"
                     /old_locus_tag="pgaptmp_002254"
     CDS             25566..26495
                     /gene="rbsK"
                     /locus_tag="ACHKNP_RS11270"
                     /old_locus_tag="pgaptmp_002254"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGU4704417.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26499..27491
                     /gene="rbsR"
                     /locus_tag="ACHKNP_RS11275"
                     /old_locus_tag="pgaptmp_002255"
     CDS             26499..27491
                     /gene="rbsR"
                     /locus_tag="ACHKNP_RS11275"
                     /old_locus_tag="pgaptmp_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HGU4704418.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27457..28884)
                     /gene="mdtD"
                     /locus_tag="ACHKNP_RS11280"
                     /old_locus_tag="pgaptmp_002256"
     CDS             complement(27457..28884)
                     /gene="mdtD"
                     /locus_tag="ACHKNP_RS11280"
                     /old_locus_tag="pgaptmp_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HGU4704419.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPVMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28907..29599)
                     /gene="yieP"
                     /locus_tag="ACHKNP_RS11285"
                     /old_locus_tag="pgaptmp_002257"
     CDS             complement(28907..29599)
                     /gene="yieP"
                     /locus_tag="ACHKNP_RS11285"
                     /old_locus_tag="pgaptmp_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HGU4704420.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7163 a         7614 c         7572 g         7363 t
ORIGIN      
        1 cagacattaa tgataattca gaatatattt atatagaaag atagatgaat ttattgatgt
       61 cagttataaa aaagggccac cggtcggcag ccctttttta tctatgatag tgattactca
      121 accgtaaccg attttgccag gttacgcggc tggtcaacgt cggtgccttt gatcagcgcg
      181 acatggtaag ccagcagctg cagcggaacg gtgtagaaga tcggtgcaat cacctcttcc
      241 acatgcggca tctcgatgat gtgcatgtta tcgctactta caaaacccgc atcctgatcg
      301 gcgaagacat acaactgacc gccacgcgcg cgaacttctt caatgttgga tttcagtttt
      361 tccaacagtt cgttgttcgg tgcaacgacg ataaccggca tatccgcgtc aatcagcgcc
      421 agcgggccgt gtttcagctc gcctgcagcg taggcttcag cgtgaatgta agagatctct
      481 ttcagcttca atgcgccttc cagcgcgatt gggtactgat cgccacggcc caggaacagc
      541 gcgtgatgtt tgtcagagaa atcttctgcc agagcttcaa tgcgtttgtc ctgagacagc
      601 atctgctcaa tacggctcgg cagcgcctgc agaccatgca caatgtcatg ttcaatggag
      661 gcatccagac ctttcaggcg agacagcttc gccaccagca tcaacagcac agttaactga
      721 gtggtgaatg ctttagtgga tgccacgccg atttccgtac ccgcgttggt catcagcgcc
      781 agatcggatt cgcgcaccag agaagaaccc ggaacgttac agattgccag cgaaccaagg
      841 taacccagct ctttcgacag acgcaggccc gccagggtat ccgcggtttc gccagactgt
      901 gacaaggtga tcatcaggct gttacgacgc acggcagatt tgcgatagcg gaattcagag
      961 gcgatttcga cgtcgcacgg aatacctgcc agcgattcaa accagtaacg ggaaaccata
     1021 ccggagttat aagaagtacc acaggcgagg atctgaatat gctcaacctt cgacagcagt
     1081 tcgtcggcgt tcggtcccag ctcgcttaaa tcaacctgac cgtggctgat gcgcccggta
     1141 agggtgtttt tgatcgcgtt cggctgttcg tagatctctt tctgcatgta gtgacggtaa
     1201 atacctttat cgcccgcgtc atattgcaga ttggattcga tatcctgacg ttttacttcc
     1261 gcgccagttt tatcgaagat gtttaccgaa cggcgagtaa tttccgcaat atcgccctct
     1321 tcaaggaaga taaagcgacg ggtcaccggc agcagcgcca gctggtcaga agcgataaag
     1381 ttttcgccca tacccagacc aatcaccagc ggactaccag aacgtgccgc cagcagggta
     1441 tccgggtgac gggagtccat gatcactgta ccgtacgcac cacgcagttg cgggatagca
     1501 cgcagaacag cctcacgcag agtaccgccc tgtttcagct cccagttcac cagatgggca
     1561 attacttcgg tgtctgtttc agaaacgaag gtatagccac gcgcttttaa ctcttcgcgc
     1621 agcggttcat ggttttcgat gatgccgtta tgcaccacca caatgtgttc agaaacatgc
     1681 ggatgcgcat tcacttctga aggttcaccg tgggtcgccc agcgagtatg agcaataccc
     1741 gtaccgccat gcagaggatg ttcttccgct gcctgagcca gcatctggac tttaccgagg
     1801 cgacgcaggc gggtcatatg accttccgca tcaacaacgg ccagaccggc agagtcatat
     1861 ccgcggtatt ccagacgacg taaaccttca agaaggattt ctgctacatc acgttgcgcg
     1921 atcgcgccaa caattccaca catagttttt gattccgatt tatatcgttg tcggtcaacc
     1981 tgtatgcccg ttttttcggg cgccccgagc cttgtagaga gtggggttat ttttatagtt
     2041 actgcttgtg ggagggagat tatgttatct cctcatccca tgtgaccgga ttacccggcc
     2101 agaatcactt tttctttacc ggacgacgcc agccttcttt ctgagtctgc ggcacacggc
     2161 tgatagctaa tgcattttcg ccgacattac gcgtcacagt tgtacccgca gcaatggtcg
     2221 cgcctttgcc tactgttacc ggggccacca gctgagtgtc ggaaccgaca aacacatcgt
     2281 cgccgataat ggtcttaaat ttattcgcac catcgtagtt gcaggtaatg gttcccgcgc
     2341 cgatgttaac gttatcgcca atttccgcat cgcccaggta agtcagatga ccagctttcg
     2401 agcctttacc cagacgcgct tttttcatct caacaaagtt accgacgtgt gcaccttcca
     2461 gcaactcagc accaggacgc agtcgggcaa acgggccaat agtacaggcc gctgccagat
     2521 tcgcgtcttc cacaacggta tacggactga tttcgcaatc atcaccaatc acgctgtttt
     2581 taatcacgca accggtgccg attttcacgc gatgaccgag agtcacgttg ccctcgatga
     2641 taacgttagt atcaatttca acatcgcgcc cgtgagtaag cgtaccgcgc aaatcaaaac
     2701 gcgccggatc gcgcagcata acgcctgcta acagcagttt ttcagcctgt tcggactggt
     2761 aaacgcgctc cagacgggag agttgcaggc ggttattcac accttctact tcgcttaaac
     2821 gttgcggatg aacggcgacg atttcacgcc cttcctgata cgccagcgca ataatgtcgg
     2881 tgatgtagta ttcgccctga gcattattgt tggtcagctt cgccagccag cgtttcatat
     2941 ctgcgccgtt agcgatcaga atgccggtgt tgatctcctg aatctgacgc tgctcgtcgg
     3001 tggcatcttt gtgctcaaca atgccggtaa ctttgccatt ttcacgggtg atacgtccat
     3061 aaccggtcgg atcatccagt ttcaccgtca gcagaccaat gccaccctgc ggtttagcat
     3121 cacgcaggcg ctggagtgtt tcgacagaga tcagcggcac gtcgccgtag agcattaaaa
     3181 tgtcttcatc atcggcaaag aaaggtgcgg cctgctgcat tgcatgaccc gtacccagct
     3241 gctctgcctg aagcacccag ttaaggttgt cgtctttcag cgcctgttta agcagatcgc
     3301 cgccgtgacc gtacaccagg tgaacgtgcg ctgcgcctaa ttcattcgca gcatcaatga
     3361 catgctgaac catcgctttc ccggcaaggg tatgcagcac tttcggaaga tcggaataca
     3421 tgcgcgtgcc tttgcctgcg gcaaggatca ctacgctcat agcattattc aacatacgcg
     3481 tcctgactgt aatttgagaa cgaatttaaa ccgcttcacc ttgaaaaaac tacatttttt
     3541 tcatcgtgaa atggacagag gataaattgt tcaataacgg attatccccg ctgacgaaag
     3601 tgccattttc gaccatcgtc acgccttttg tctgcagaaa attaaggcag taccttcatt
     3661 taagcgtcag gtggatgttt ttgctcttat ttcgatcaat gaataaacgg gaaaacaggg
     3721 gtgttttata tcagcaggat ctatgtgaac gctcttcagg acggatcacg cgtaaaaaaa
     3781 agccagcctg tttccagact ggcttttgtg cttttcaagc cggtgttaca tcgctttttt
     3841 ggtcaactcg ataacgcgca gctgcgcgat cgctttggcc agttccgcag acgcctgagc
     3901 gtaatctacg tcgccgtgag agctgctaat gtgctcttca gccttacgtt tcgcttccat
     3961 ggctcgcgct tcgtcgagat cctggccgcg aattgcggtg tcggccagaa cggtcacgtt
     4021 gccaggctgc acttcaagaa tgccgccaga cagatagata aactcttcgt gaccgtgctg
     4081 tttcacgatg cgaatcatac caggcttaat ggcggtgagc agcggtgcgt ggccagggta
     4141 gatccccagt tcaccttcgc tacccgttac ctggattttc tcgaccagac cagagaacat
     4201 ttgttgctct gcgctgacga cgtccaggtg gtaagtcatt gccatatcac cctccgatta
     4261 aggcgttaaa gttttttggc tttttccaca gcttcttcga tggaaccgac catgtagaac
     4321 gcctgctccg gcaggtgatc gtattcgcct tccatgatgc ctttaaagcc acggatggtg
     4381 tctttcaggg agacgtattt acccggagaa ccggtgaata cttctgccac gaagaacggc
     4441 tgggacagga agcgctggat cttacgagca cgcgctacca ccagtttgtc ttcttcagac
     4501 agttcatcca tacccaggat ggcgatgatg tctttcagtt cctgataacg ttgcaggatg
     4561 gactgaacgc cacgcgcggt gtcgtagtgt tcctgaccaa ccaccagcgg gtccagctga
     4621 cggctggtgg agtccagcgg gtcaacggcc gggtagatac ccagagacgc gatctgacgg
     4681 ctcagtacca cggttgcgtc aaggtgcgca aaggtggttg ccggagacgg atcagtcaag
     4741 tcatccgcag gtacgtatac cgcctgtacg gaggtgatag aaccagtttt ggtggaggtg
     4801 atacgttcct gcagaacgcc catctcttcc gccagggtcg gctgataacc taccgctgaa
     4861 ggcatacggc ccagcagtgc ggatacttcc gtaccggcca gggtgtaacg atagatgttg
     4921 tcaacgaaca gcagaacgtc acgaccttcg tcacggaatt tctcagccat ggtcagaccg
     4981 gtcagagcaa cgcgcagacg gtttcccggc ggctcgttca tctggccata caccagggat
     5041 actttgtcga taacgttgga gtcggtcatt tcgtggtaga agtcgttacc ctcacgagta
     5101 cgttcaccta cgcccgcaaa cacagagtaa ccggagtgct cgatcgcgat gttacgaatg
     5161 agctccatca tgtttacggt tttacctaca cccgcaccac cgaacagacc aactttaccg
     5221 cccttagcga acggacacat caggtcgata actttgatac cggtttccag cagttcctga
     5281 gagtttgaca gctcttcgta ggaaggtgct gcgcggtgaa tcgcccaacg ctcttcttca
     5341 ccgatctcgc ctttcatgtc gaccggttca cccagtacgt tcatgatacg gcccagagtc
     5401 gctttaccta ccgggacttc gatcgggtgt tcgaggtctt ttacatccag accgcgacgc
     5461 agaccgtcgg aggaacccat tgcgatggta cgcacgatac cgccgccgag ctgctgctga
     5521 acttccagca ccagacgctc attaccattt tgcacctcaa gagcatcgta cacgcgcggt
     5581 acggcatcct gagggaattc gacgtcaact acggcgccga ttacctggac aatctttcca
     5641 gtagccatct taaatcctct acgaaataac ctgtttaaac cgcggcggcc cccgagacga
     5701 tctcggtgag ttcctgagta atgctggcct gacgagcttt gttgtatacc aactgcagct
     5761 ctttaatcag gctgccgcca ttgtcggtcg cggctttcat cgccaccata cgggcggcct
     5821 gctcgctggc caggttttca accacgccct gataaacctg agattcgaca taacgacgca
     5881 gcagggtatc cagcaacgcc ttcggatcgg gttcgtacag gtaatcccag gatttatgtt
     5941 tcagatcatc atcatctgat gccggtaacg gcagcagctg gctgatggtc ggaacctgag
     6001 acatggtgtt aataaatttg ttgctgacaa tgtaaagctt gtccagacgg ccttcgtcgt
     6061 aggcctgcaa catcactttt accggaccga tcagttcgga cagggaaggg ttatccccca
     6121 tgccggtgac ctgggcaaca acattgccgc ccacggagtt gaagaacgac acgcctttcg
     6181 agccgatcat tgcgaggtcg cattgaacgc ctttgtcggt ccaggtcttc atttccgcca
     6241 gcagtttttt gaacaggtta atgttcaaac caccgcacaa accacggtcg gtcgacacca
     6301 ccaggtagcc cacgcgttta acgtcgcgtt cttccaggta tgggtgctta tattccagat
     6361 taccgctcgc aaggtgacca atcactttgc gcatggtatc tgcataagga cggctggccg
     6421 ccatgcgatc ctgcgattta cgcattttgg aagcggcgac catctccatc gctttagtga
     6481 tcttttgcgt gttctggacg cttgcgatct tactacgtat ctcttttgcg ccggccatga
     6541 gcttctcctc aatgccttgc ggcctgccct aaggcaagcc gccagacgtt accaggattg
     6601 ggttgctttg aaggaatcga ggatgccttt cagcttgcct tcgatttcgt cgttgtagcc
     6661 accggtctgg ttgatctctt gcatcaacgg agcgtgatca cggtcgacgt aagccagcag
     6721 agcggcttcg aagctgccga ttttcgacag ttcaacatcc accaggtaac cacgttctgc
     6781 tgcgaacaga accagagact gttgcgctac ggacatcggc gcatactgtt tctgtttcag
     6841 cagttcggtc actttctgac cgtggtcaag ctgcttacgt gttgcatcgt caaggtcgga
     6901 tgcaaactga gagaacgctg ccagttcacg atactgtgcc agagcggtac ggataccacc
     6961 ggacagtttt ttcatgatct tggtctgtgc tgcaccacca acacgggata cggaaatacc
     7021 cgggttaacc gcaggacgaa taccggcgtt gaacaggttg gtttccagga agatctgacc
     7081 atcggtaatg gagattacgt tggtcggaac gaacgcagaa acgtcacccg cctgagtttc
     7141 aataatcggc agtgcggtca gagaaccggt tttccctttc acttcacctt tggtgaaggc
     7201 ttcaacgtat tcggcgttaa cacgcgcagc acgctccagc agacgagagt ggaggtagaa
     7261 tacgtcgccc gggaatgctt cacgtcctgg cggacgacgg agcagcaggg agatctgacg
     7321 gtaagcaaca gcctgtttag acaggtcatc gtaaatgatc agcgcatctt caccgcggtc
     7381 acggaagtat tcgcccattg cgcaaccggc atacggagcc agatattgca gtgcagcgga
     7441 ttcagacgcg gttgctacca caacgatggt gttagccagt gcgccgtgct cttccagttt
     7501 acgtaccacg ttagaaatgg tggacgcttt ctggccgata gcgacataga tacatttgat
     7561 accggaatcg cgctggttga tgatggcatc gatagccagt gcggttttac cggtctgacg
     7621 gtcaccgatg atcaattcac gctgaccacg accaattggg atcatggagt caacggcttt
     7681 ataaccggtc tgtaccggct gatctacgga ctgacgttcg ataacgcccg gagcgattgc
     7741 ttctacagca gagaagccgt cgtgatccag cggaccttta ccgtcgattg gtgcacccag
     7801 agtgttaacc acacggccca gcaggccacg gccaaccgga acttccagga tacggccagt
     7861 acacttaact ttcatgcctt cggcaaggtc agcgtacgga cccataacaa ccgcacctac
     7921 agagtcgcgc tcgaggttca gtgcgatagc gtaacggtta cccggcaggg agatcatttc
     7981 accctgcata caatcggcca ggccgtgaat gcggataaca ccgtcactta cagaaacaat
     8041 agtaccttcg ttgtgagctt cactcacaac attgaactga gcaatgcgct gcttgatcag
     8101 ttcgctgatt tcggtggaat tcagttgcat gctccagtcc ccttaagact gcaagacgtc
     8161 tgcaaggcgc tcaagacgac cgcgtacgct gccatcaatg accatatcac ccgctcggat
     8221 gataacgcct gccattacag acttatcgat tttgcaattc agcttaactt tgcgtgacag
     8281 acgtttttcc atcgcagcag aaattttcgc gagctgttgt tcactcagtg cggcagcgga
     8341 aatgacgtct acctcagcgg tagcctcact cacggcacga aggtgaataa actgctccag
     8401 aacatccggg agcgcgttaa gacgaccatt ttcagccatt acccgaatca ggttctgacc
     8461 gttttcgtcc agttgctcac cacaaactgc gataaacgac tcggcgagcg tttctggcgc
     8521 aagcgcgcca gagagaagct ctgccatttg ttcgtttttg gttacctcgg cggcaaacgc
     8581 cagcatgtcc tgccagcgtt ctacactttg gtgttcgacg gcaaagtcaa aagctgcttt
     8641 ggcgtagggg cgagctaccg taataaattc agacatcagc ccctccctcc ttacagttca
     8701 gcgacaagtt tatccacgat gtcgctgtta gcagcttcat ccacggaacg ttcgatgatc
     8761 ttctcggcgc cagcaacagc caggatagca acttgcttac gcagctcttc acgggcacgt
     8821 ttacgctcgg cttcaatttc tgcctgcgcc tgggccacga ttttagtacg ttcctgttct
     8881 gcctcagctt tcgcttcgtc cagaatctgc gagcggcgtt tgttcgcctg ctcgatgatt
     8941 acctgggctt ccgctttcgc ttttttcagc tggtcggtcg cgctggcctt tgcaaggtca
     9001 aggtccttat gtgctcgttc tgcggaagca aggccgtcag caatttcttt ttgacgtttt
     9061 tcgatggctg ccattaatgg cggccatacg tacttcatgc agaacagaac gaacaggaca
     9121 aacgcgatgg cctggccgag gattgttgcg ttaagattca cagcacaatg cctctattta
     9181 gttaacgttc tgatattgct ctttaaataa aagcaacgct tactacgcga cagcgaacat
     9241 cacgtacaga cccagaccta cagcgatcat cgggatagca tccaccagac ccataacgat
     9301 aaagaactga gtacgcagca gaggaatcag atcaggttga cgcgctgcgc cttccaggaa
     9361 tttacccccg aggatgccga taccgatcgc agcaccgatt gccgccagac ccatcatcac
     9421 agcggcagcc atgtacagca gatccatatt caggttttcc atgacagtct ccagtttgtt
     9481 tcagttaaaa cgtagtagtg ttggtaaatt aatgttcttc agacgccatc gacagataga
     9541 cgatcgtcag aaccatgaag atgaaggctt gcagcgtaat gatcaggatg tggaaaatgg
     9601 cccacggcac attcaggatc cactgtgacc accacggcaa cagaccagca atcagaatga
     9661 aaatcagctc accggcatac atgttaccga acagtcgcaa accaagtgaa actggtttgg
     9721 acagcaggct taccccttca aggattaagt tgacaggaat gaacgcccag tgattgaacg
     9781 gctgcagcgt caactctttc gtgaagccgc cgatgccttt cattttgatg ctgtagaaca
     9841 gaatcaggat aaatacgccc aatgccatag acagcgttac gttcacgtcc gcagacggaa
     9901 ccacacgcag tgcaggcaga cccagtacat gttcagcaat gtacggcagc aggtcgatag
     9961 gcagtaaatc catcaggttc atcaggaata cccagacgaa gatcgtcagg gccagcggag
    10021 caatcagctt gcttttgcca tggtacatgt ctttcacgct accattaaca aagccgatca
    10081 ccagctcaat cgcggtctga aacttacctg gcacaccgct ggtcgccttt ttggctacgc
    10141 tacggaataa aaccaggaac aacagaccca gcaccaccga gaagaacatg gagtcaatat
    10201 tgattgtcca gaaggtggct ggggggtttt gtggatccac cagcgagaat gtacgcaggt
    10261 ccagctgaag gttattcagg tggtgtccta tgtaatcctg cggcgtcata ttttctgaag
    10321 ccatgatgcc ttttaccctt tgttgttaat tacagccggt gccagtatct gaaccaccag
    10381 caccaaaacc cacgtaacga tcagcggcaa gaataccgcc tttaaaaccg ccaacgccac
    10441 caccagtaac accaacatcg ccagaacttt gaaagcttcg ccaaatgcga atgtccaggc
    10501 cacccggcct ttcgctggtg tatgcgcctg gtgacgccag gcaaatatca taaacaaaac
    10561 gttaggcaga aagactgcca ggcccccgct tattgcagag acgccccaga aggggtcttt
    10621 gaggctgaac agcaatccac ttgctatcac caccagtaac tgaacgagca gaagcttccg
    10681 agcaacgttt cgactcacga gcgacacaga catcacgttt ttcactcctg ctcccttcga
    10741 ggtatgccgc gtgtcgtata aaactttctt taaggcttag agtcaagcat caaaaagcgg
    10801 tcaaattata cggtgcgccc ccgtgatttc aaacaataag tagccaaaag gtgaataaat
    10861 gtttaaatat ttttccagtg catacaattg cgacttttct gctaaccctg ttcgatcatg
    10921 aaaaactgta aataacgcgt aaacactggt gataaagcgt gcttcagatc acatattgcg
    10981 catgttcgcg cacagcatat ttatttactt ggcaaatgat gcctttgcaa gtttatgata
    11041 tttcagtcta aaaacagata ctgttttaat aaatgacatt tacacaacaa aaaccaccca
    11101 ttgacatttt taataatgtt ttaacagcca atgatggttc ttagcgccga tttttagcag
    11161 actgatattt tcactaatga cttattttct gcttaccaaa aaaagccacg ttatcttgtt
    11221 gatgcaaaag agtgaacgtg gcgttaaatg taaccagtta tatcagtaga aaacctggtt
    11281 gttgttaaca gtctaaccgg tcaatttttt atgatttttt tgataaaaat taaattttat
    11341 ttgctttaat caccaccaga tgacgttcgc catccaaggc tggaacctga agtttaacca
    11401 ctgattcgac ctgatattct tcgggcaaca aagcgatttc atcttccggc atttgccctt
    11461 tcagcgcgta gaaatggcct tgctcaccag gaagatggtg gcaccagctc accatatcgt
    11521 tcagagaggc aaaagcgcgg ctaattacgc catcaaatgg cggctctgaa ggaaactctt
    11581 ctaccctgct ctgtactggt tcaatattct ccagtttaag ctcatgttgc acctgacgaa
    11641 ggaaacgcac gcgtttacca aggctatcca acagagtgaa atgggcttca ggacgcacga
    11701 tagagagtgg aatgcctggc agtcctggtc cggtgccgac atcgataaac cgttcacctt
    11761 gcagatacgg tgccaccaca atgctatcga gaatatggcg taccagcatc tcattaggat
    11821 cgcggaccga agtcaggttg tacgctttgt tccatttatg cagcatattc acgtaggcaa
    11881 taagctggtt tttctggtga tcggtaagcg aaatacctgc gtctttcagc agtaaggaga
    11941 gtttgttgag cacggtgatt acctgttctt gatgcgttgc ctggtaagcg ggtgcttacc
    12001 aggcattttt aatgcgttat gcgctacgac gcagcatacc ctgttttttc agccacacca
    12061 gcagaatgga gatggccgca ggcgtgacgc cagaaatacg cgaagcctgg ccgatagagg
    12121 ctggtttgtg atcgttaagt ttggcgatca cttcgttaga aagaccggat acctggcggt
    12181 aatccagtgt cgcgggtagc agggtgttct cgttacgcag ctgcttttcg atctcatctt
    12241 gctggcgcgc gatataacct tcgtatttaa cctgaatctc aacctgttcc gccgcctgtt
    12301 cgtctgtcaa cgcaggggca aacggcgtca gcgtggttaa tttttcataa gtcatttccg
    12361 gacgacgcag cagatcttca ccactggctt cacgggaaag cggcgcagtc aggtgagcat
    12421 tcacttcggc tgcagcttcc gccgacgggg ttacccaggt cgatttcaga cgctgacgct
    12481 cacgctcgat attctcaagt ttctcgttaa agcgcgccca acgttcgtca tccaccaggc
    12541 ccagttcacg accgatttca gtcaaacgca gatccgcatt atcttcgcgt agcatcagac
    12601 gatattctgc gcgcgaagta aacatacgat acggttcttt ggttcctaaa gtgcacaggt
    12661 catcaactag tacgccgaga tacgcctgag aacgtgccgg agcccaacct tctttgtcag
    12721 cagacagacg ggcagcgtta agaccggcca gcaaaccttg cgcagcggct tcttcgtaac
    12781 cggtagtgcc gttaatctga ccagcaaaga acagcccctg gataaacttg ctctccagcg
    12841 tcggtttcag gtcgcgagga tcgaagaagt catactcaat ggcataaccc ggacgcacga
    12901 tcttcgcgtt ttccatcccc tgcatagagc ggacgatttg catctgcaca tcgaacggca
    12961 ggctggtgga gataccgttc ggataaattt cattagaggt cagtccttcc ggttcaagga
    13021 agatctgatg ctgatttctg tcggcgaagc gcataacttt gtcttcgatc gacgggcagt
    13081 agcgtgggcc gacaccttcg atcacccctg catacattgg gctacgatcg aggttactgc
    13141 ggatcacatc atgggttttc tcgttggtat gagtgatata acacggcacc tgctggggat
    13201 gctgggacgc attgcccata aacgagaata ccggcattgg gttatcgcca tgctgttgcg
    13261 ccagcacact aaagtcgata gttcgagcat caatacgcgg tggtgtcccg gttttcagac
    13321 gaccaacgcg cagcggcagt tcacgcaaac ggcgagaaag cggaatggac ggcggatcac
    13381 cagcacggcc accgctgtaa ttatccagac cgatatgaat tttaccgtcg aggaacgtcc
    13441 caacggtgag cacgacggct ttggcacgga acttcagtcc catttgggta acagcaccga
    13501 ccacgcgatc gttttcgaca ataagatctt caaccgcctg ctggaagatc atcaggttcg
    13561 gttggttctc cagcgccgta cgtaccgcct gacggtagag cacacgatcc gcctgagctc
    13621 gggtagcgcg aaccgccggt cctttgcttg cgtttagtat cctaaactgg atacccgctt
    13681 gatcgatcgc tttcgccatc agaccgccga gtgcatccac ttcttttacc agatgtccct
    13741 tcccaatacc gccgatcgcc gggttgcagc tcatctgccc cagagtgtcg atattgtgtg
    13801 tcaaaagcag agtctgttga cccatacgcg ccgcggccat cgcggcctcg gtgcctgcat
    13861 gacccccgcc aatgatgatg acgtcaaaag gatccggata aaacatggtg attgcctcgc
    13921 ataacgcggt atgaaaatgg attgaagccc gggccgtgga ttctactcaa ctttgtcggc
    13981 ttgagaaaga cctgggatcc tgggtattaa aaagaagatc tatttattta gagatctgtt
    14041 ctattgtgat ctcttattag gatcgcactc cctgtggata acaaggatcc ggcttttaag
    14101 atcaacaacc tggaaaggat cattaactgt gaatgatcgg tgatcctgga ccgtataagc
    14161 tgggatcaga atgaggggtt atacacaact caaaaactga acaaccgttg ttccttggat
    14221 aactaccggt tgatccaagc ttcctgacag agttatccac agtagatcgc acgatctgta
    14281 tacttatttg agtaaattaa tccacgatcc cagccattct tctgccggat cttccggaat
    14341 gtcgtgatca agaatgttga tcttcagtgt ttcgcctgtc tgttttgcac cggaattttt
    14401 gagttctgcc tcgagtttat cgatagcccc acaaaaggtg tcatattcac gactaccaat
    14461 accgattgcg ccaaagcgga ccgcagaaag atcgggcttc tgttcctgca atgcttcata
    14521 gaaaggagaa aggttgtccg gaatatctcc ggcaccgtgg gtggagctga taatcagcca
    14581 gatccccgag gcagataaat cttccaacag cggaccgtgc agcgtttcgg tggtaaaacc
    14641 cgcctcttcc agcttttcag ccaggtgttc tgctacatat tcggcaccgc cgagggtgct
    14701 gccgctgata agagtgatat ctgccataaa ccgccacctt tattaagagt ggcgtattgt
    14761 acgctgtgaa cgcgttggga tctacctgtg gaaaagtatg ggattaaaaa agccgattat
    14821 ggcttgatgg tacgcatgat cgggttttgc aggacgatca atgtctcggt ggactgaatt
    14881 tcatcaattg tttggatctt gttgataagt acatgctgga gagcgtcgat cgaacggcac
    14941 atcactttta taaagatgct gtagtggccg gttgtgtaat aggcttcagt gacttcatca
    15001 aggctttcca gctttgccag cgcggaaggg tagtctttgg cgctctttaa tataatgccg
    15061 ataaagcagc ctacgtcata accgagctgc ttcgggctga catcaatacg cgccccggta
    15121 atgatccccg cctgcttcat tttctctact cgaacgtgaa tcgtccccgg actgacgcca
    15181 aattgtttcg ccagttcggc gtaagcggtg cgcgcattgc ccattaatgc ttccaggatg
    15241 ccacggtcca gattgtcgat cagataattt tccataggat tttcttatgc agattgatga
    15301 ttcattctat tttagccttc ttttttaatg aatcaaaagt gtgttaggct ttttattgaa
    15361 tgattattgc atgtgtgtcg gtttttgttg cttaatcata agcaacagga cgcaggagta
    15421 taaaaaatga aaaccgctta cattgccaaa caacgccaaa ttagcttcgt gaaatctcac
    15481 ttttctcgcc aactggaaga acgtctgggg ctgatcgaag tccaggcgcc gattcttagc
    15541 cgtgtggggg atggcacgca ggataacttg tcgggctgtg aaaaagcggt gcaggtaaaa
    15601 gtgaaagctc tgcctgatgc ccagttcgaa gtggttcatt cactggcgaa gtggaaacgt
    15661 cagaccttag ggcaacacga cttcagcgcg ggcgaagggc tgtacacgca catgaaagcc
    15721 cttcgccccg atgaagagcg tctttctccg ttgcactcgg tctatgttga ccagtgggac
    15781 tgggaacgcg taatgggcga cggtgagcgt caattctcga ctctgaaaag cacggtagag
    15841 gcgatctggg cgggaattaa agcaaccgaa gctgcagtta gcgaagagtt tggtttggca
    15901 ccgttcctgc cggatcagat ccacttcgta cacagccagg agttactgtc tcgttatccg
    15961 gatctcgatg ccaaagggcg tgagcgggcg attgcgaaag atcttggcgc ggtattcctc
    16021 gtcgggattg gcggcaagct gagtgatggt catcgtcacg acgtgcgcgc accggattat
    16081 gatgactgga gcaccccgtc agagctgggc catgcgggtc tgaacggcga tattctggta
    16141 tggaaccctg tactggaaga tgcgtttgag ctttcttcca tggggatccg cgtggatgcc
    16201 gacacgttga agcatcagtt ggcgctgacc ggtgatgaag atcgtctgca gctggaatgg
    16261 catcaggcgc tgctgcgcgg tgaaatgccg cagaccatcg gcggcggtat cggccagtct
    16321 cgtttgacca tgctgctgct gcaactgccg catatcggcc aggttcagtg tggagtatgg
    16381 ccagctgctg ttcgcgagag cgtcccttct ctgctgtaat aatttatcgc cgccagcgtc
    16441 tgagcaggcg gcttcgcatc ccggtatcaa agcgccagat atgatcgaaa atgcgcatga
    16501 tgccgggttt gccgtgtgcc gacatcgcca cggcatgaaa gcgatgctga tgtacccgct
    16561 gcagctcttt cactttactc gtcacgtcgt caggcaaccg ctgagcgata aaatcagaaa
    16621 tcaccaccgc atcggcatca aaccattccc tgctttgcaa gcgttccata atggcgcgaa
    16681 aacaactggc aagatcggtg ccgccacgaa actgctggct taaaaaacgg attgcttgtt
    16741 cgatgccttg tgggcctgaa agctcataac ggacgatctc ggtggaaaat agcataatat
    16801 agcagcgccg gttttcagcg agagcaatgc gcatcaaggc caggcagaac gctttcgcac
    16861 actgttcatt aaagccgccc attgagccgg aagtatccac acagacaata aacggcccgc
    16921 gcggctgttc atcgtaatct ttatgtacca ccggacgttc gatcactttt tcacgccacg
    16981 actcaccgtg caggcgatag gtgagcaact gtttttccac cagccgacgg taaaactcat
    17041 actccagttc cgttatccct agtgtcgcca gttctggcgg cagaagacgt aaaatatcat
    17101 cgctttgttg cagaccatca acctgctcag gaaccgtcgc cggttcgcgc accatggtgc
    17161 ggaaggtttc catctgcgca tcgttgcgcg gtattgattt ggcttcccga gaacgcccca
    17221 gctgctctgc caggcgtttc agttccggct gttcgttaag aaattcaccg tatttcacaa
    17281 tcaactgata gtcgccacgt ttaagctgac cggcgctcat atcccacaga cgaccagctg
    17341 cagtattgtt atctgcgaga atcggttcaa gttgtccgct cagcgtcatg cgttcctgaa
    17401 cttcactcaa cagttgttcg cgttcttctt ctaatagctg ttgatttaac gtcgttgctt
    17461 gcacgatcag acttaaacgc caacgctgga gaaaaagcgt gtgtaacgct gaagtgatcg
    17521 tgctgttagc atcaaccaac tgtcgggctt gttctgccca tggagaattc agacgatgca
    17581 gtaagtccag gatctgtggt agctgcacaa taaactgtgg cgtggagagg agctggcttt
    17641 gctgatagca catcacctct tcggtgagtt ccggcgggac tcgggcatct ttcagccgac
    17701 tgcgcagcgc ttcacgccag cggggaacat catcagtgat tgccgccttc aggcgtggga
    17761 atttttcaaa gaagaccgcc agctgcggtg aggccagcag tgcgatgatc atctcttcga
    17821 tcaatccctc ttcgctgacg gccagcatca cattaagcgt atccagcgtt agcattgttg
    17881 tgcctggcga atctgtgcac cgacatcctg taggctggct tcaatgcgac ctaaccagtc
    17941 gccagggata aacagacatt tttgctgttc gctgaataaa gcgtgttgct tgcgccagtc
    18001 gctttcaagc tcttccagtt gttgttttat ttcaccaggc agaccttcag ccgatgaacc
    18061 gggaagtgcc agcgtactgc cttgtaaact tacatcgcga acgacaagat gttgggcgct
    18121 atcaacttcc agattcagtt tctgggcaaa gccgataccg ttcagtttac cgcgaatttc
    18181 accacctttg ctcagccact gttccagcgc gctacgctca aaggagatat gaaccacttc
    18241 catatcatgc agttttaacg gtttttgcag cagtagagtc agagtggaag cagtaacgtt
    18301 aacaggaagt tgatactgct gacgacggct gaaaatgccg cccagacgaa ttaccgttaa
    18361 ggctgtttta tcgctttgtt gctgctggag ttgcaggtga cgttgcacaa tcgcgccaag
    18421 gcgagtcaac atcccttgct gttgccaggc gtgaccggtc atcaatacat caatttgttg
    18481 ttgtatcaaa ttcaggcttt gcgcgtcata ccacaggcaa tctttcagca aaatgagatc
    18541 taccggggca acagcactgc gaccgctaaa aaaggcgctg gcctgcaata atcggatcgc
    18601 ttttttccag cgacgatccg agacataagg cgcatccggt aatttatcca gttgctggcg
    18661 cagcataaaa atcagctcaa atacatgatc gggcagcgta atttcaccaa tctctttctg
    18721 ccagcgttca tattcttcat ctgtgacctg caaggcatca ggaaccggat tgtcgttttc
    18781 atcctgttga ctggtcagca tggagcggaa attcgcttta tcctgcactt tatctaacca
    18841 cagacgaatc agcatgcggt catataacgc ttccagactg ctgtctgctt ccggcagctc
    18901 gttggaggcc gccaccagca gacgcatcgg gattttttct acgtgtgcac cgttgcggaa
    18961 ctggcgctcg ttaatggcgg tgagcaaggt attaagaatt gccgggcccg ctttccagat
    19021 ctcatccaga aagacaattt ctgcttccgg caggtaaccg ctggttaaac gttcatagcg
    19081 cccttcatct tttagcgcct gaatagaaag gggaccaaaa acttcttccg gcgtggagaa
    19141 gcgggtcatc agatattcaa acgcgcgggc attctgaaag gcgaatttta agcgccgggc
    19201 gatcaaactt ttggcaatac ctggcgggcc aaggaggaac acactttcac cacttaatgc
    19261 cgctaataaa cacaagcgga tggcgtggct acgttcataa agcccctttt ccaacgaact
    19321 gctcaggcgg gaaattcttt ccgctaataa atgagggtga gccataatga agtggcgtcc
    19381 tttcgtcaaa agttctgcgt aaattgcgag tatagacgtt tcttgctggt ggctaaaata
    19441 gtctcaaagg gggggtattt ttctttgagc caggttaatg tggccgcatt taggagtacg
    19501 attttgccgt taatcgtgca tactgtgcgc ttttttgtgg gccaagggac taagcacaca
    19561 tttcatattt caacgaaaga ctagtctatg agcactgata ataagcaatc attgcccgcg
    19621 attaccctcg cggcgattgg agttgtctac ggcgatattg gtaccagccc gttatataca
    19681 cttcgtgaat gtttgtccgg ccagtttggt tttggcgttg aacgcgatac cgtgtttggc
    19741 tttttatcgc tgatcttctg gctgctaatc tttgtggttt ccattaaata tctcaccttc
    19801 gtgatgcggg cagataacgc cggtgagggg gggatcctga cgttgatgtc gcttgccggg
    19861 cgtaatacgt cggcgcgaac cacatcaatg ctggtgatta tggggctaat cggcggcagc
    19921 tttttctatg gtgaagtcgt cataacaccc gctatttcgg tgatgtcagc cattgaaggt
    19981 ctggaaatcg tcgccccgca gctggatacc tggatagttc ccctctcaat tatcgttctc
    20041 actttattat ttatgattca aaaacatggc acggctatgg tcggtaagct gtttgcgccg
    20101 atcatgctga cctggttttt gattctggca ggactggggt tacgtagcat tattgctaac
    20161 ccggaagtgc tgcatgcgct gaatccaatg tgggcggtgc atttcttcct tgaatacaaa
    20221 acggtttctt ttattgcatt aggggcagtg gtgttgtcga ttacgggggt cgaggcgctg
    20281 tatgctgata tggggcactt tggtaagttc cctattcgcc tggcgtggtt caccgtcgta
    20341 ttaccttcct tgacccttaa ttacttcggt cagggagcgc tgttgttaaa gaacccggaa
    20401 gcgattaaga acccgttctt cctgttggca ccggactggg cgctgatccc gctgctgatc
    20461 atcgccgcac tggcgacggt aattgcctcg caggcggtta tctctggcgt cttctcattg
    20521 acgcgtcagg cggtacgtct gggatatttg tcgccgatgc gcattattca cacctccgaa
    20581 atggagtcag ggcaaatcta tattccgttt gtgaactgga tgctctatgt cgcggtcgtg
    20641 attgtgattg tcagctttga gcactccagc aacctggcgg cggcgtacgg gattgcggtg
    20701 accggaacca tggtgctgac gtctattctc tcgactaccg tggcacgtca gaactggcac
    20761 tggaataagt attttgttgc gctgatcctg attgctttcc tttgtgtcga tattccattg
    20821 ttcaccgcta acctcgataa actgctctcc ggcggctggt tgccattgag cctcggtact
    20881 gtgatgttta tcgtgatgac cacctggaag agcgagcgtt tccgcttgct gcggcggatg
    20941 catgaacatg gtaactctct ggaagcgatg attgcttcgc tggagaaatc accgcctgtt
    21001 cgcgtgcccg ggaccgcggt gtatatgtcg cgtgcaatca acgtcattcc ctttgcgctg
    21061 atgcataacc ttaaacataa caaggtattg catgagcggg tgattctgtt aactctgcgt
    21121 accgaagacg cgccatatgt ccataatgtc cgtcgggtac agattgaaca actgtcgccc
    21181 actttctggc gcgtggtggc aagttatggt tggcgagaaa cgccaaacgt ggaagaagtt
    21241 ttccaccgct gcggtctgga aggattaagt tgccggatga tggaaacctc cttctttatg
    21301 tcgcatgagt cgttgatcct cggcaaacgc ccgtggtatt tgcgtctgcg cggcaagctg
    21361 tacttgctgc tgcaacgtaa tgcgctgcgt gcaccagatc aatttgaaat tccgccaaac
    21421 agggttatcg aactgggtac tcaggtcgaa atctaacgcc agacgcctcc tttctccata
    21481 agggggcgtt tttgttttca tggttaatca ccatgtaaaa cgtttcgagg ttgatcacat
    21541 ttccgtaacg tcacgatggt tttcccaact cagtcaggat taaactgcgg gtcagcgaaa
    21601 cgtttcgctg atggagaaaa aaatgaaaaa aggcaccgtt cttaattctg atatttcatc
    21661 ggtgatctcc cgtctgggac ataccgatac gctggtggtg tgtgatgctg gtttacctat
    21721 ccccaaaagt acaacgcgta tcgatatggc attaacccag ggtgtacctt cttttatgca
    21781 ggtgctgggc gtcgtcacaa atgaaatgca ggtcgaggct gccattatcg cggaagagat
    21841 caaacaacat aatccgcaac tccacgaaac gttgctcact caccttgagc agctgcaaaa
    21901 acaccaggga aataccattg aaattcgtta caccacgcat gaacaattca aacaacaaac
    21961 cgcagaaagt caggcggtaa ttcgcagcgg agaatgttct ccgtatgcga atatcattct
    22021 ctgtgctggc gtgacgttct gaggccgtca tggaagcatt acttcagctt aaaggcatcg
    22081 ataaagcctt cccgggcgta aaagccctct cgggcgcagc gttaaatgtc tatccgggcc
    22141 gcgtgatggc gctggtgggc gaaaacggcg cgggtaaatc caccatgatg aaagtgctta
    22201 ctggtatcta tgctcgcgat gccggcacgc ttttatggct ggggaaagaa acgacattta
    22261 ccgggccgaa atcttcccag gaagccggga ttgggattat ccatcaggaa ctgaacctga
    22321 tcccgcagtt gaccattgcc gaaaacattt tcctcggtcg tgagtttgtt aatcgctttg
    22381 gcaaaattga ctggaaaacc atgtatgccg aagcggataa attgctggct aaacttaacc
    22441 tgcgctttaa aagcgacaag ctggtgggcg atctttccat cggtgaccag caaatggttg
    22501 aaatcgccaa agtgctgagc tttgagtcga aagtcatcat tatggatgaa ccgaccgatg
    22561 cgctgaccga taccgaaacc gaatccctgt tccgcgtcat ccgcgaacta aaatcgcaag
    22621 gccgcggtat tgtctatatc tcccaccgca tgaaagaaat cttcgagatt tgcgatgacg
    22681 ttaccgtttt tcgtgacggg caatttattg ctgagcgcga agtggcatca ctgaccgaag
    22741 attcgctgat tgagatgatg gtgggccgca agctggaaga tcaatatcca cacctggaca
    22801 aagcgccggg agatatccgc ctgaaagtcg ataatctctg cggacctggc gttaacgatg
    22861 tctcttttac tttacgcaaa ggcgaaattc ttggcgtctc tggtttgatg ggcgcaggtc
    22921 gtaccgaact gatgaaagtg ctctacggcg cactaccgcg caccagcggt tacgtcaccc
    22981 tggatgggca tgaagtcgtt acccgttcac cgcaggatgg cctggcaaac ggcattgtgt
    23041 atatctccga agaccgtaaa cgtgacggtt tagtgttggg catgtcagta aaagagaaca
    23101 tgtcgctgac agcgctgcgc tacttcagcc gcgctggcgg cagtttgaag catgccgatg
    23161 aacagcaggc tgtgagtgat ttcattcgtc tgtttaatgt gaaaacaccg tcgatggaac
    23221 aggcaattgg tctgctttcc ggtggcaatc agcaaaaagt ggcgattgcc cgcggtctga
    23281 tgacacgccc caaagtgttg atccttgatg aacctacccg tggcgtagat gtcggcgcga
    23341 aaaaagagat ctatcaactg attaaccagt tcaaagccga tggcttgagc atcattctgg
    23401 tgtcatcgga gatgccagaa gtattaggca tgagcgatcg catcatcgtc atgcatgaag
    23461 ggcatctcag cggggaattt actcgtgagc aggccaccca ggaagtgtta atggctgccg
    23521 ctgtgggcaa gcttaatcgc gtgaatcagg agtaaaaaaa tgacaaccca gactgtctct
    23581 ggtcgccgtt atttcacgaa agcgtggctg atggagcaga aatcgcttat cgctctgctg
    23641 gtgctgatcg cgattgtctc gacgttaagc ccgaactttt tcaccatcaa taacttattc
    23701 aatattctcc agcaaacctc ggtgaacgcc attatggcgg tcgggatgac gctggtgatc
    23761 ctgacgtcgg gcatcgactt gtctgtaggt tctctgttgg cgctaaccgg cgcagttgct
    23821 gcatctatcg tcggcattga agtcaatgcg ctggtggctg tcgctgccgc tctcgcgtta
    23881 ggtgccgcaa ttggtgcggt aaccggggtg attgtagcga aaggtcgcgt tcaggcgttt
    23941 atcgctacgc tggttatgat gcttttactg cgcggcgtga ccatggttta taccaacggt
    24001 agcccagtga ataccggctt tactgagaac gccgatctgt ttggctggtt tggtattggt
    24061 cgtccgctgg gcgtaccgac gccagtctgg atcatgggga ttgtcttcct cgcggcctgg
    24121 tacatgctgc atcacacgcg tctggggcgt tacatctacg cgctgggcgg caacgaagcg
    24181 gcaacgcgtc tttctggtat caacgtcaat aaaatcaaaa tcatcgtcta ttctctttgt
    24241 ggtctgctgg catcgctggc cgggatcatt gaagtggcgc gtctctcctc cgcacaaccc
    24301 acggcgggga ctggctatga gctggatgct attgctgcgg tggttctggg cggtacgagt
    24361 ctggcgggcg gaaaaggtcg cattgttggg acgttgatcg gcgcattaat tcttggcttc
    24421 cttaataatg gattgaattt gttaggtgtt tcctcctatt accagatgat cgtcaaagcg
    24481 gtggtgattt tgctggcggt gctggtagac aacaaaaagc agtaataacg actacaggac
    24541 atcttgaata tgaacatgaa aaaactggct accctggttt ccgctgttgc gctaagcgcc
    24601 accgtcagtg cgaatgcgat ggcaaaagac accatcgcgc tggtggtctc cacgcttaac
    24661 aacccgttct ttgtatcgct gaaagatggc gcgcagaaag aggcggataa gcttggctat
    24721 aacctggtgg tgctggactc ccagaacaac ccggcgaaag agctggcgaa cgtgcaggac
    24781 ttaaccgttc gcggcaccaa aattctgctg attaacccga ccgactccga cgcagtaggt
    24841 aatgctgtga agatggctaa ccaggcgaac atcccggtta tcactcttga ccgccaggca
    24901 acgaaaggtg aagtggtgag ccacattgct tctgataacg tactgggtgg caaaatcgcc
    24961 ggtgattaca tcgcgaagaa agcgggtgaa ggtgccaaag ttatcgagct gcaaggcatt
    25021 gctggtacat ccgcagcccg tgaacgtggc gaaggcttcc agcaggccgt tgctgctcac
    25081 aagtttaatg ttcttgccag ccagccagca gattttgatc gcactaaagg tttgaacgta
    25141 atgcagaacc tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa
    25201 atggcgctgg gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc
    25261 ggatttgacg gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact
    25321 atcgctcagc tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg
    25381 aaaggcgaga aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag
    25441 ttttaatcag gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt
    25501 aatataaaga aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc
    25561 cgaatatgca aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc
    25621 ttaatcttca atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg
    25681 catttggcgg caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg
    25741 cgtttattgc ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca
    25801 ctgataacat tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc
    25861 tgatttttgt taatggcgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg
    25921 ccctttcccc ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat
    25981 taatgcagct ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa
    26041 ataagactat cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg
    26101 cgctggtgga cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg
    26161 ttgaaaatga tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta
    26221 ctgtactgat tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc
    26281 gcgttcctgg attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg
    26341 gcgcgttaat cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc
    26401 atgctgccgc tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg
    26461 aagagatcga cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg
    26521 cccgcctggc gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg
    26581 tcagtgaagc gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat
    26641 cagccctggc gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg
    26701 ccagtaccaa tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac
    26761 gcggttatag tctcgtcctt tgcaataccg aaggagatga acagcggatg aatcgcaatc
    26821 tggaaacgct gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc
    26881 aaccttcgcg tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg
    26941 ctccgttcga tggcgacagc gatcttattc aggataactc gttgctgggc ggagacttag
    27001 caacgcaata tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg
    27061 ataaaactcc ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc
    27121 tcaacattcc tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg
    27181 ccatgcgcca actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg
    27241 ctatggctgt tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata
    27301 tcgcggtgat tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca
    27361 ctatccacca accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga
    27421 taacccagcc gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg
    27481 gttcggctta gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag
    27541 catgaacatt gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa
    27601 ttgttcgaca gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac
    27661 agcaacgcct aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct
    27721 ggcgttgtca tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga
    27781 catagccatc cctaatataa acaacggcaa gatcagcatc catatcgcca ttaccggtga
    27841 ttgcaaagag aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg
    27901 gcgatagccc agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa
    27961 cgctgtcggt gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa
    28021 catcaatggc ataaggaacg gtacacaacc ggtccccaga cgggttgcaa tattgcctac
    28081 gataccgatc gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt
    28141 gtgtcgcgca tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa
    28201 ggcaatccag ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac
    28261 aaggctgagg ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa
    28321 attgggcata tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa
    28381 aaatatccag tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat
    28441 tggccccacc agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg
    28501 ataagcgcgc agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg
    28561 aataacccgg aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc
    28621 caatgtgaac agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag
    28681 ccatccgctt accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg
    28741 catcgcgaga ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat
    28801 agtggcatca agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat
    28861 actgcgcttc tttttatcgc tcattcaatg tcctattatc gggttgtcac ttatcaggtg
    28921 agcgtagcag cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat
    28981 cgacaatcgc ctggtgcaga tccagcttta tcactgtgtc gctggtaatt gacgtgaagt
    29041 aagtgtgata aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca
    29101 tttcatagat atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt
    29161 tctcttttaa tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg
    29221 tgccaaccgt tgcagccagt aggcaagctt gcggctccag acagatacgc ataaccagaa
    29281 agtgatcgat gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat
    29341 caagaaaatt ccagtttgat tgtggcatga cccgggtacc aattcgcggt cgcggtaaaa
    29401 ccatcccttt tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa
    29461 attgctcgcc cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta
    29521 agatgcgttg cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact
    29581 gttgtgcgct taaaggcatt actaatcttc ctttttcttt ttattcctcc ttagtatgcc
    29641 accaggaagt gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gcacattaac
    29701 ggggcttttg ct
//
LOCUS       DAZFWL020000047        29625 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFWL020000047 DAZFWL020000000
VERSION     DAZFWL020000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.047"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHKNP_RS11290"
                     /old_locus_tag="pgaptmp_002258"
     CDS             complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHKNP_RS11290"
                     /old_locus_tag="pgaptmp_002258"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HGU4704421.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(804..1352)
                     /locus_tag="ACHKNP_RS11295"
                     /old_locus_tag="pgaptmp_002259"
     CDS             complement(804..1352)
                     /locus_tag="ACHKNP_RS11295"
                     /old_locus_tag="pgaptmp_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HGU4704422.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHKNP_RS11300"
                     /old_locus_tag="pgaptmp_002260"
     CDS             complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHKNP_RS11300"
                     /old_locus_tag="pgaptmp_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4704423.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHKNP_RS11305"
                     /old_locus_tag="pgaptmp_002261"
     CDS             complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHKNP_RS11305"
                     /old_locus_tag="pgaptmp_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HGU4704424.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLANYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVELDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQITEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHKNP_RS11310"
                     /old_locus_tag="pgaptmp_002262"
     CDS             complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHKNP_RS11310"
                     /old_locus_tag="pgaptmp_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HGU4704425.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHKNP_RS11315"
                     /old_locus_tag="pgaptmp_002263"
     CDS             complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHKNP_RS11315"
                     /old_locus_tag="pgaptmp_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HGU4704426.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHKNP_RS11320"
                     /old_locus_tag="pgaptmp_002264"
     CDS             complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHKNP_RS11320"
                     /old_locus_tag="pgaptmp_002264"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HGU4704427.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11026..11805
                     /gene="elyC"
                     /locus_tag="ACHKNP_RS11325"
                     /old_locus_tag="pgaptmp_002265"
     CDS             11026..11805
                     /gene="elyC"
                     /locus_tag="ACHKNP_RS11325"
                     /old_locus_tag="pgaptmp_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HGU4704428.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGDGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11782..12675)
                     /locus_tag="ACHKNP_RS11330"
                     /old_locus_tag="pgaptmp_002266"
     CDS             complement(11782..12675)
                     /locus_tag="ACHKNP_RS11330"
                     /old_locus_tag="pgaptmp_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HGU4704429.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHKNP_RS11335"
                     /old_locus_tag="pgaptmp_002267"
     CDS             complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHKNP_RS11335"
                     /old_locus_tag="pgaptmp_002267"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HGU4704430.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHKNP_RS11340"
                     /old_locus_tag="pgaptmp_002268"
     CDS             complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHKNP_RS11340"
                     /old_locus_tag="pgaptmp_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HGU4704431.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHKNP_RS11345"
                     /old_locus_tag="pgaptmp_002269"
     CDS             complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHKNP_RS11345"
                     /old_locus_tag="pgaptmp_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HGU4704432.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHKNP_RS11350"
                     /old_locus_tag="pgaptmp_002270"
     CDS             complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHKNP_RS11350"
                     /old_locus_tag="pgaptmp_002270"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HGU4704433.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHKNP_RS11355"
                     /old_locus_tag="pgaptmp_002271"
     CDS             complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHKNP_RS11355"
                     /old_locus_tag="pgaptmp_002271"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HGU4704434.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17843..20107)
                     /locus_tag="ACHKNP_RS11360"
                     /old_locus_tag="pgaptmp_002272"
     CDS             complement(17843..20107)
                     /locus_tag="ACHKNP_RS11360"
                     /old_locus_tag="pgaptmp_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HGU4704435.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHKNP_RS11365"
                     /old_locus_tag="pgaptmp_002273"
     CDS             complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHKNP_RS11365"
                     /old_locus_tag="pgaptmp_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HGU4704436.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHKNP_RS11370"
                     /old_locus_tag="pgaptmp_002274"
     CDS             complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHKNP_RS11370"
                     /old_locus_tag="pgaptmp_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGU4704437.1"
                     /translation="MTESFAQLFEDSLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHKNP_RS11375"
                     /old_locus_tag="pgaptmp_002275"
     CDS             complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHKNP_RS11375"
                     /old_locus_tag="pgaptmp_002275"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGU4704438.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHKNP_RS11380"
                     /old_locus_tag="pgaptmp_002276"
     CDS             complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHKNP_RS11380"
                     /old_locus_tag="pgaptmp_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HGU4704439.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHKNP_RS11385"
                     /old_locus_tag="pgaptmp_002277"
     CDS             complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHKNP_RS11385"
                     /old_locus_tag="pgaptmp_002277"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGU4704440.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHKNP_RS11390"
                     /old_locus_tag="pgaptmp_002278"
     CDS             complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHKNP_RS11390"
                     /old_locus_tag="pgaptmp_002278"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HGU4704441.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26972..27664)
                     /locus_tag="ACHKNP_RS11395"
                     /old_locus_tag="pgaptmp_002279"
     CDS             complement(26972..27664)
                     /locus_tag="ACHKNP_RS11395"
                     /old_locus_tag="pgaptmp_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGU4704442.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHKNP_RS11400"
                     /old_locus_tag="pgaptmp_002280"
     CDS             27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHKNP_RS11400"
                     /old_locus_tag="pgaptmp_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HGU4704443.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         7194 a         8057 c         7192 g         7182 t
ORIGIN      
        1 tcccctgcaa tattttgaat ttgcacgatt ttgtaggccg gataaggcgc tcgtgccgca
       61 tccggcataa acaaagcgca ctttgccaga aatccaggcc cgcattaaaa gcgggctttt
      121 ttatgtgcct taccagacgg gcatttcgtc ttgcaggaag ggattatgca ggcgttcata
      181 accaagtgtg gataatggtc cgtgacccgg aataaatgtc acgtcatccc ccagtggcag
      241 caatttatct ttgattgaag aaatcagttg attatgatcg ccacgcggga agtcactgcg
      301 ccctactccg cctttgaaaa taacatcgcc agaaatcagc agctttgccc gatcatcaaa
      361 aaacacgaca tgacccggcg tatgcccagg gcaatgtaac acctgtaaag tcacattccc
      421 tatgctgatg gtatcgcctt cgttcagcca acgatctggc gtcagcggct ggcactcttc
      481 cagaccaaac atacgacttt gcgcaggcaa gccttgcagc cagaactcat cttctttttc
      541 cgggccaaaa accggcacgc cgtaatgttg cgccagttcc gccgctgcgc caacgtggtc
      601 cagatgacca tgcgtcagca ggatctgcat cagtgtcagg ccgctgtcat caacttcctg
      661 tttgattttt tccgcatcgc cgccaggatc gaccagtgcg gccagacggg tttgttcaca
      721 ccagattaat gaacagttct gggagaatgc ggtgaccgga ataatacgat agttcatact
      781 gcccctgttt cgttaagcaa ttactaccag tgccgtgctg gcccggtatc aatatgcaca
      841 aagttactac gtggataata tcctacacca cctgcgcgca tagataacgc ggctttgcga
      901 atattgctta acgcgatacc ttcaatatgg aaatccatcg cctggccttt agtgtgatag
      961 cttttcttcg ctactccacg gctgcgggcg cgtagttcat tgttggtatc aatagaacga
     1021 taaccggaaa tgagttgcac cggtttgcgc gtgcctaaca acccttgcag gcgatacaac
     1081 tggtcgaata atcctgggtc gatggacttt attttgttcg cgcggtaatc gcggaaaaaa
     1141 tggttaagtt ttgccaattc ttcctgaata tagcctctgc catcgaaaaa ctccgctttg
     1201 attgactctc cggtatgaag attattgagt gtcaaaatgc gcgggcgtgg ggtagagagt
     1261 gttgcaaacg caggggtcgg caggatggcg gcaccgagtg caacgccacc aagcgccagc
     1321 aatttgcggc gattagcgtc gaatttgtcc atgataatca agtctacagg tcaatgttat
     1381 cgtttatatg cacttctggc gcacaagagg caccttaacc gcccgtaaag cagacgtcaa
     1441 gatgactcaa ccccagcagt gacgggggct gcagagaatc gcccccaatc atgctaatta
     1501 ttacgacaac tgatttcccc gaactacttc atttacctga ttaattgttc cgctttcgat
     1561 acgatttgcg agctggatcg cgcaggcaga tcataattgt aaatatctgt acgatactgg
     1621 gtacgaccat ctgcaccaac aaaggccgtc aggtagtaga gattcaccgg aatcgactgc
     1681 cgaatattga cgtaacgtgt atcaccctgc ttcagcgcat cagaaatacg tttgtcattc
     1741 cagcctgcat cctgcaacag catattcgcc agatcggaag ctttattcac tcgtacacag
     1801 cctgagctca atgcgcgtgt atcacgcttg aacagattgt ggttcggcgt gtcatgcaaa
     1861 taaatggcct ctgaactcgg catattgaat ttatagcgcc ccagcgagtt ccgtgggcct
     1921 ggagcctgct ggaagcggaa cggtaaattc gaggccgtga ttgtagacca gtcaacctgc
     1981 catgggtcaa tcgcttctct gctgttccag ccgcgcatca ccgtatagcc atggctttcg
     2041 agatatcccg gatcgttgcg cacttttggc agaatatctt tgcgtgccag agttggaggt
     2101 acgttccacg gcgggtttac cactacgttg ttaagggcac tgctcatcat cggcgttttg
     2161 cgatcggggc gaccgacaat gactcgcgaa tccagcacct gattgccgtt ctgatagtag
     2221 accagcgaat aggccggaat gttaaccatg atcccggtag aaagctctgt tggcagcaag
     2281 cgcaatcgct ggatgttgag agccaacacg ccagcacgct gggcgggcgt tacgtttaac
     2341 cagtcacgtg ttgccgggcc aatagcacca tctgccccca atccttgcca tgcctgaaaa
     2401 cgtttaacgg cttccaccag ttcattatcg taggcggcac gaacggcagg cgcaggttta
     2461 ctacgagacg tcgtttgctt gtccatcggc ttagtttctg ctgtttcaac agtaacagcg
     2521 gatgggctga ctaccgcgtc agttggcgtg tcatcgccag gtagagtaat tttcggcccc
     2581 ccgtccaaca tgcctgtgcg ttgcaatatt tcgcgcaacg ccggtacgtc gttactccac
     2641 tgccctgggc gcaacgttgc tttgccggtc agttggggcc acggtttggt gtcactgagt
     2701 aaggccagta acgattcatg catcgccgca tattgcggat gctgcggtgc cagtcctgca
     2761 acaaacgtag gcaattgacc tttatccagc gccagctgcc attggttaat caccgagagc
     2821 ggcggcgttg caagtgcata aggtttactg ctatatagcc agcgagtgcc tttgaccgga
     2881 atatttgcaa tgaaatggag atagcccatc atcgcatcag agagcactac gtcgcgtgcc
     2941 atcccgttaa caccaggatc ggtcagtaac tctacccatt tattaaactg cggctggaaa
     3001 ccggcaatcg ccacctctgc cagctgttgc tggaaggctt taacagcatc acggttttcc
     3061 cacatgggtt gcatatcgcg tgcggcatac aacagttgaa gctggttaag ataaaccggc
     3121 ttgtaacctg cgggtaattg agattcgatt tgcgtgcggg ctttttctgc cgcaccttca
     3181 ggcaatggct ggatccccgc cattattgcc gtggcttgcg cctgcggcag tgcctcgccc
     3241 tgttcactga cagccaccgg gctgtcgcca gggattactt caggctcatc ggcctgcgca
     3301 ttgaacagtg gagcgaatgt tacggccagg cacaaactga ttgccgacag ccgacgacca
     3361 cacatcatat taagcaacat cccttgcccc ctgtttttat catactctga atagccaggc
     3421 tgttatttta cgacttgcct gtacaacgcc aatactcacg aaagtatgac gagcaagcct
     3481 gttactcatc aggcttatct tcagtatata aacgccgata cgtttttaca taacctaatg
     3541 taaagaagtt taaagaaaag cgcgccgtaa tgtgccactt ttttagtgcg gaaacggagt
     3601 tttcggaaaa agaaaaggcg gcattgctgc cgccttaatt ttaactcgcc tgagaaggcg
     3661 cttcgtcact tcctggaagc gtttcaggga gttgcggcgc aaatcctcgc aggccgacga
     3721 cgtgaacgtg ttcggtattc tggaagactt tacgcaccag tttataggtg gtgcctttct
     3781 ccgggctgat attttccggc gctgcgatga tgagctgcat ttgcaaacgc tcacacaatt
     3841 caaacagcgt ggcgatagaa cgagcatcca gtcgcgctgc ttcatcgagg aacagcaggc
     3901 ggcaaggaga gatatcttta ccgcgcaggc ggcgagattc atcttcccag ctttgtacca
     3961 ccatcaccag aatcgacata ccggtaccaa tcgcctcacc ggtcgacaat gcaccagact
     4021 ctgcgcgcaa ccaaccatcg gaaccacggt taacctcaac ttccatttcc agatagttgc
     4081 ggtaatccag cagttcttcg ccaatggtct gcggcgtgcg ctgtcccata tcaatctgcg
     4141 gattaagacg ctgatacaat ttcgccaacg cttccgagaa ggtcagacgg ttgctgttaa
     4201 acagatcctg atgctgctcg tgctgttcag agagcacatc cagcagcatg gcgtgcgttt
     4261 cacgcacgtt cacgttgagg cgcacgctgt tcacctgacc aaacgatacg ttctgcaacc
     4321 cctggttgag catacggata cggttctgct cgcgctgaat ggttttgcga atgatgttcg
     4381 ccacgctgcg ggaactgatc gccagtttct gttcacggga ggttaattct tcggtcagac
     4441 ggctaagttc gatctccatc tgttcgatag cttccaccgg atcatcggta cgaataatat
     4501 cctgacgaat acgttcacgc agatgctgat aaaccgccac gaagaactga attttacgct
     4561 ccggacgttt cggatcttcc gacatgcgca gcacgtcgcg cagatgttcg ttatccgcca
     4621 ccgccagacg cagcgcaccc aatgccttat ccgacataga gcgcaaatca tcagcggaga
     4681 gataagccag ctcacgacgg tgtaagcggc gctcaacgcc gttatctttc accatgcgca
     4741 tcaccgcaca ccagcccgct ttggcggtca ctacctgctc gcgcatctca aagtaatccc
     4801 gctccagctt gcgcagtttg cgggtcaggt tgtccatctc cgcttcgcag aaggtaagcg
     4861 ctttttccag ttgattgcgg cgtgaacggt tattgctcag ttgcgcatgc agctcgtcac
     4921 ggcgaatacg cgcccgctct tctgccccgc tatcagcacg cacgccgata tcctgcaatt
     4981 cacgttgcag atcgttgagt agctcttttt tggtgtcgta agaacttttc agcgaagcca
     5041 gcacctggtt gtactgactc aactgcgctg cgtgaccacg caacgcttca cgagcgcgag
     5101 tacgctccgc ttccgcctgt tccagacgtt cacgcagctt ttcgttgaga tcgctattac
     5161 cgctcagcat ttctgccgag tcagaatagc taaagtgcgc acgacgctgc accacttccg
     5221 tcagggcaaa cgcctgctga cgggcatcgc gctgcatctg ctgagagtac gcgtaatctt
     5281 cttttagctg ttcgaactgt tccgggtcgc tctgcaatac cgaaacgatc ggttccagtt
     5341 tcgccagttg attgccaaac tgctgaacaa aacgcgcggc ttcctgggct tcatccagac
     5401 gttcgcggat ttcatcgacg cgatccgcca ggctgtcatc agccaacagg ttgagacgcg
     5461 gcagaatgcg gttcagcgcc gtaacgcctt ctttcgcctg ctcaaactga atacgctgct
     5521 gctggttatc attttcatga ttactcaacg cccgctccag ttcgacgcga cggctgttca
     5581 gttgacggat ttctgcttcc gggtcagact caaacgcaac cgccagatga ctgccgataa
     5641 agcggctgaa cgcctgatgc agacgctgag ttttctgtac atcaaaggag agcgtggcga
     5701 agcgttcgga aagcacttca cgctcggcat ggaggctttc aatacggctt tcacgcgcag
     5761 cacgaccaaa cagcggcact tccgggaaac gtgaataacg ccactgacga tcggcgattt
     5821 tcaccactac cgctttttcc agctcatcaa cgctgaacac gctgtcatcg aatgactgcg
     5881 gatctccttc gatcagatag agatcttccg ggcaatcggt caagccttcc aggtgttcag
     5941 ttatctgtga cagatctggc accacgatgg cgtggcgtga cgggccatac agcgctgaga
     6001 agtacggcgc atcttccagg ctaacgtcgt cataaatttc tgacagcagc acaccaccaa
     6061 aacgctccgc cagcgcgttc agacgctgat cttcagagcc gccaggctgg cttaaacgtt
     6121 cgatctcttc atcgacggcg tttttgcgcg cgcccacttc atcgagttca acaatcgcct
     6181 ctcgctcacg ctccagcaac tgttgcagat attcggtgac gtcctggctg gaggtaaact
     6241 cttcgccgca ctgttcgctc aactggttga gactgttttg cgctgccagc caaaccggcg
     6301 cacgctgcat caaactctga atgcgagact gcagctgttc ctgttcctgg cgcagtgcca
     6361 tgcgctcttc acgggcgtta gacacgctat cggaaagaga ggcaatgcgc gcttccagct
     6421 cctgatgcag agcttccagc tcgtcaatgt caaaattctt gccctgacgt ttgcagaaat
     6481 ctgccagcag acgctcagct tcttgctgtt cgcgcagacg ctgttccagt tcgcttaagc
     6541 gcatccgcaa cggctgaacc tgctctgcca ggtgacgctg atcgacccct tcgcgcaata
     6601 gttcgcgagc gacatcccac gcctcgttac gcgccagcgg gccgttgatt gccaccacca
     6661 gctgataagc ctgctcaaac tggctgtgcg cggtttgcgc catgctcatt ttctgctcaa
     6721 gagagagcat tttttcagtc gcttccagct ctttcgcctg gaaggtttcc agccattcgg
     6781 cggcgctgtc ggcggttaag tccggcagat ggcacagttc tttggcgcga ttaagcgcag
     6841 caatcgcctg gttatactgg atcgcgcgcg tttgctggac gtccagcgcc tgctggtagt
     6901 tagcaagctg gcttttcagc tcgtccactt ccagttcggc agcttccgcg cgagcctcgt
     6961 tctcttcctg gcgttcgatg gcttctgcca ccacttcgtt ttgctcttcc agacggatct
     7021 gcagctcatc gagatccgct tcgtagcgtt cgatcttctc ctgctgacgc agtgcggttt
     7081 gcaccaggtt caggtgatcg ctggccgcct gataatccgc ttccagatcg ccttcggcac
     7141 cgttgtgctc tgccagctca cgcgccatat cgacgtgttt gtactgctca gccgccagtt
     7201 gctgacgcga agtatgtaac tcgcgacgaa actccagggc tttgtcgaga tggacacggc
     7261 gctcgttggc gtgacgcatg tagtccgccg ccacgtagtt ggtagcttcg ctgatcagat
     7321 gcttaaacag gtcacggtcc gactgggtga cacgaatcgc ttccagcgtc atgcggtttt
     7381 cacgcagcgc cgcttccatg tcctggaacg ctttacgcac gccgctgttt tccggcaaca
     7441 ggtagtcgcg cagagaacgg gtaatggcac tggagatccc gccatacagc gaagcttcga
     7501 tcagacgata gaatttgcta cggtcagatg cggagcgcag acgacgcgcg atgatgccca
     7561 gatcgaacat cagcgagtgg taatcggtaa tggagttgaa ctgtttaaac tgcacgcctt
     7621 ccatcgcctc gagcttgtct ttcagctcgt taagcggcag cacgcgcgcc tggcgttcgt
     7681 tcagggtttc ggtcaccagc tgtgtcggct gcaccgacat cggcagtccc tgaatggcaa
     7741 atggtttgat atccacttta cgatcgcgtc cggcaacctg ttgcagacgc acaccgacca
     7801 ccacgcgctg gtggcgcgag ttaatggtgt cgagcatcga ataacagaca cccgctttca
     7861 gcttaccgtg cagaccttta tcgcgcgaac cgctggtggc cccggcttcc gtagtgttac
     7921 ggaaatgcag cagggtcagg tcggggatca gcgccgtaac gaacgccgcc atggtggtgg
     7981 atttacccgc cccgttaccg ccagaaagcg tcgtgaccag ctcgtcaagg tcaaaagttc
     8041 gggcaaaaaa gccgttccag ttaatcagcg tcagtgagcg aaatttaccg cgttcaatca
     8101 ttattcttcc tctccgctat ctggctgatt ttcttcggtt tcatcgttga gttgcagatg
     8161 attttcaatc ggcattgctt cgccatcacg aatcaggcga cgctgtgctt cacggggatc
     8221 gtcgccagca cgcacatcgg ctccgaagcg gaacaccgat tcggtaatgc gaaacttgct
     8281 gctgtcgtgg cccataaacc acaccatgcc taaacgacgc agacggttga gcgaagaacg
     8341 tactttctcc tgcaacttct gacggtcaac gtctgaaccg gttgaacggt tgttcaccag
     8401 tttcagcagt tttgcttcat cggccagggt gagcagttcg tcgtacagtt cctgctgggt
     8461 gaaaatcccc tcattcgcca gccgttccgg gctgagatag agataacaga ggattttccc
     8521 gaccatcata tccagttccg acaagacgga acgagggatc agcgtggtgg aacgtgggcg
     8581 taaatagaag aacccttctg gtgcgcgaat aagctcaacg ttataacgcg cgtaaaactc
     8641 ttccagatat tcctgaaaat ccatcaggaa tgcatgatta tccagttcgt cgaggccaat
     8701 atggcggcct gaacgtaagg cgctgtccag cgccggaaat aacggattcg ccagcgcctg
     8761 cgccagctta accggcatca cttgttcaat atttgtcgat gacatgcgcc tgtaccttgg
     8821 ctccgtaatc attaatcggc tgccatttcg ctggcagtcc ggtgaaatct gcttgcgcta
     8881 cgccaagacg taccgcctga tcaataacaa tacgcgcaac gtcaaagtga cgtgcacgcg
     8941 gatactgtga cagatattcg cgtaccacca gaccaagatc cagcggcact tgtctggttt
     9001 tgtacacggc aagttgttct tcgatgatcg ccgccagctg ttcgcggatc tcgttaaact
     9061 cttcgtattc cagatcctca ggaagttccc cagtcacttc ttcatcgcgc agtgccatct
     9121 cttcgtcacg catatccagc agacgatcgg cattggcata agttagcgcc cacggctcat
     9181 caaaataggt ttgtaccgac tgacgtaacc gctgagcaaa gacgcggttt ttatccatat
     9241 cgatcgcggt acgaataaat ttgtgtacgt ggcggtcgta gccaatccac aagtcgatgg
     9301 attgctggcc ccaactgata atacgatcga gtttgctctg cagatcgaac accagacgat
     9361 cgacgaaatg cagatcgtca tgggtcatcg tcgcatcctg aatgcgcaac agattagcct
     9421 gcaatttgtc gcctgccgct tccagcgtat cctgcaattc acgcagcgtc ccggaagttt
     9481 ccgaaagcaa caattcacag ctggaaatcg ccgcccgcca gtctttgttc agcaactggg
     9541 cgatatcgtc cttcacctgc tgctgctgtt cgtccatcag acgttgcgtc aggtcgatac
     9601 tgtcgaaaat ttctgctacc gaatatttca gtggcgcata gacattacgg tgccagtgaa
     9661 attcatcacc gccctcttcg gcggcatctg ctgcgcgttt gagctcaccc gccacaatcg
     9721 acaactgcat agaaagacgc agcgtagaaa actcgcgctg acggatgtag tagtcagtaa
     9781 tgccgatgcc gagcggcgtc agacggtaaa ttgcgttccc ttccgcctgc tcgctggtaa
     9841 agcggttcag cagacgttga cgcaccatgt cgttgatcgc gttattggcg cgcacgccga
     9901 tggtttcgct ggtttgctca aacgcatcac tcacatggcg gaatgcatcc accagctcgc
     9961 cttcactcat ctcaccatcc agacgctcgc cgttcagcgt ggcaaccgcc agcagaaaag
    10021 agagtcggtc taccggcagc gagatggaga agtcattttt tctggcccag gcaaccagtt
    10081 cggggactgt ctgggaaaat tcactcatac tttatccttg ctctgcggtt tgcgcgcggt
    10141 gacatgaata taacgcccca gggtaatata cggttcctga cggcaataac gcgtttctaa
    10201 ttcaagtaat gcttcatagc agtcgcgctg ctggtgcttc tcgcgcagat aatcatgaaa
    10261 cacgcgaacg cctgtcttac ccataatttg ccaaccagct tcttccagcc acagataaac
    10321 ctgcgccggg tcgcgtggat aatctggcga aagcgtccgt tttttctttt tcggcattcc
    10381 cgcctgcacg taatcaaaat tcccggcgac catgttatgc atcaacaaac catgcgcatt
    10441 gtagaacatt aacgacaaca cgccgcctgg acgcaacact gaccacaggg tctgcaatac
    10501 actgcgggga tcggccaccc actcgagcac cgcatggaac aatatcagat caacgggcgt
    10561 ttccaaatgc gaagcaacat cctgagcggc gcaatgtata aattgcatgt tgtcgctcac
    10621 accttttgct tctgccgcct gttttgcgcg gtcgatcatc tgcgcagaaa gatcgcataa
    10681 aatgacctga tgcccacgct cggccatttt gattgcggtc tgcccttctc caccgccagc
    10741 atccagcaca cgcagttttt gcgggcccat ttccgccagc acgcgatcga gatcctgcca
    10801 cagaatagcc tgtcgaagct gccctttggt ggtgccgtaa atgttacggg aaaacttttc
    10861 cgcaatatca tcaaaattgc gatcctgcat tgctttctcc accggttggc taagacgttt
    10921 tctgataaag acgctatttt gtcacagcca cggcgagaat gaacccgtct ggtgtaatat
    10981 cgctggcgat cccttgctat atggttaaaa aaggaaccag aaaggatgct ttttacactg
    11041 aaaaaagtga ttggcaatat gttgctgcca cttccgctga tgttgttaat tattggcgct
    11101 ggcctggcgc tgctgtggtt tagccgtttt caaaaaaccg ggaagatttt tattagcatt
    11161 gggtggctgg cactcttgtt attgagtttg caaccggtag ccgaccgcct gctgcgtccc
    11221 atcgaaagca cctatccgac atggaataat tcgcaaaaag tggactatat cgtggtgctc
    11281 ggagatggtt atacctggaa cccgcagtgg gcaccgagct ctaatttaat caataacagt
    11341 ctgccacgcc tgaatgaagg tattcgctta tggcgcgaaa atccgggatc aaaactgatc
    11401 ttcacgggag gcgtagcaaa aaccaatacg gtgagtacag cggaagtagg tgccagagtt
    11461 gcgcaatcgc tgggcgtgcc gcgcgagcaa attatcaccc tggatttgcc aaaagatacc
    11521 gaagaagaag ctgcggcagt gaaacaggcg attggtgacg cccctttcct gctggtgacc
    11581 tccgcatcac atctgccgcg ggcgatgatc ttttttcagc aggaagggtt aaatccgcta
    11641 ccagccccgg cgaatcagtt ggccatcgac tcgccgctta acccgtggga acgggcaatc
    11701 ccttctccgg tctggctgat gcatagcgat cgtgttggct atgaaacgct tggccgcatc
    11761 tggcagtggc tgaaaggtcc gtcaggcgag ccacggcaag agtgattttg atgccagatc
    11821 gaagttgcgc cgactaaatc gtccggtatt aaccagttgc gcaacttcat cccataacac
    11881 atacaaccaa cgccgccaga tgaacgactc cgccactggc gcgcgttgca ggtaactcca
    11941 gagcaaatct tccgccagag aattatccat tagtcggaac agttcgtatt ctcgcggtgc
    12001 ccaaagcatt agtcccggcc cgaccatcgc cagtaactga tcgctgcgcg aatctttcag
    12061 catgctgcgt aaacagaagt taccgtgaat caacacacaa ttgtcgttaa agccttcaaa
    12121 taatgccggg agacattcgc gagtgcgaaa caggatccgc ttatcctgca tcgtcagacc
    12181 ggtgttattg aactgattga gcgtggtcca tagcacttca acatgttgca ggtaccatga
    12241 gggccagaaa ttttcctgag tattgtcgac cgcgccgacg caaccgcgac tgtcctgacg
    12301 gtgccaggcc agtaaggctt caacgatttg gtctttgagc tgttcccagc gttctggtgt
    12361 tcgggctggt gcctccaccg aaacaccacg catccgctcc agtagcagga catcagggcc
    12421 ggggtgttct tcatgcgtca tcacaccata aatagtcggc atacggacag tcccacttct
    12481 tgccagcatg gtggttttcc atgccagttg tcgggctttt ccgggcgtac taaagcttct
    12541 tgccattaac ggcattgggt ttccctggct gtcatacaaa gcccacaacg ccgtatccgc
    12601 tttttcattg acgcactcaa tacgactgag tttttcgccc agtaaatggc ttaattcggc
    12661 acgcagctgt tccattcaga ttaccctcat aaaagctact gctttcataa tgagcgccaa
    12721 tataaaagat gtcacctgaa tgagatcaat tgttgaaaag aaaaattgag gaaaatcgta
    12781 acggtacgac actcctccca aaattggctg aagtgtcgtg aagtgaaatt agcgcatttc
    12841 agcgcgaacg cgctcaagat cttcaggggt atccacacct gtgccaggaa cttcctgagc
    12901 aacagcaaca tggatttttt cgccgtacca cagaacacga agctgctcta acatttcgat
    12961 gtgttctaac ggacttgcct gccagttgac gtaacgacgg ataaagcctg cacggtagcc
    13021 ataaatacca agatgacgca ggaagttatc gccaacggtt tcaaggcctt ctgcaaaacg
    13081 atcacgatcc caaggaatgg tggcgcgtga gaagtacagt gcataccctt cagcgtcgag
    13141 aaccactttc accgcattcg ggttaaacgc ttcttccgca ttgtggattg gcaccgccag
    13201 agtcgccata cccacctgac gctgagcgag gttatcagca acctgacgaa tgattgtcgc
    13261 agggatcatc ggttcatcac cctgcacatt aacgatcacc gtgtcgtcgc tgaatgcgca
    13321 tttttcgaca acttccgcca gacgttctgt tcctgactga tgatcggcgc gcgtcataca
    13381 tacttcaccg ccagcggctt caacggcgcg ggcaacatcc tcatgatcgg ttgccacgat
    13441 gatgcgctcg gcacctgatt cacgcgcgcg ttcaagaaca tgaacaatca tgggtttgcc
    13501 gttaatatca accaatggtt taccgggcag acgcgtggac gcgtagcgcg cgggaataat
    13561 gaccacaaaa ctcatgattt actctcatca gcagtcagca cgcgggcttc cgtttccagc
    13621 aacactggaa tgccatcgcg gagcgggaaa gccaggttat ccagtttgca aataagctct
    13681 tgtttttcct ggttatacca aagttttccg ttgcaaaccg ggcaggcaat gatttcaagc
    13741 aaacgatgat ccatagttcc tccagatgga tcgggttatg aatgcataaa tcttatcata
    13801 atcatttatg cgacggggtc tatttcccag ccagtgcggc aatcagtaaa gagaccttgc
    13861 gggcagcgtc ctaatgtcac ccgcgttgcc tgctgccagc tcgcgaaatc agtaatcgcc
    13921 tgacgtaacc ctttttgcag cattgtcgtt ggtttaatgc cttcctgtaa ccacagagag
    13981 ataacttcaa ggatgcctgt ctggcgatgc attttggcat ccattcgccc aactaattgc
    14041 ccacgatgta ataatggcag aacaaaatag ccatactggc gtttcggcgc tggggtatag
    14101 cactccagcc ggtagctaaa atcaaaaagc tgctctgcgc gtttgcgatc ccagacaaca
    14161 ggatcaaaag gcgaaagtac cgcgctgtgc gttgcagtga gctttccggc cagcgcacgc
    14221 tcgagtagcg gcagcaaatc agcatgcagc caaagattgc ccaatttttc aacatgcaca
    14281 gcaatgattt gctgctgttc agccctcgct tcgcgccacg ccgccagtgc cggacgtttc
    14341 agccgatagt aatctgccag ccactgttcg cggaatattc ccaggctacg cgcactgtta
    14401 tccagcatga tgatttctgc ttctgtttgc gaaacgagat cgcgctcatc atcccagtca
    14461 ggcatgacac ggtgggttaa atcataaacg cgctggaagt tgcgccgttc aatcaccatc
    14521 acctttccgg cagtaaataa accttccaga tgccgtttat gcggcttcca ttcccaccag
    14581 ccgcttgcac ctttacgagg atgctcaaaa tcggctgaac gtaccggccc cttatcatga
    14641 atatgctgaa ttaactgtgc aatttccgcc tcatgttcct gcatccaggc gtctttgtat
    14701 ttccagccca ttttttcagg tgccagcatg cggtggcgaa taagacgaaa gtcgctacgc
    14761 ggcataaagc aggcttcatg cgcccagtat tccattaatt cgccacgcgc cagagactca
    14821 tccagccact gggcaggata atttcccaga cgactgaaaa gcaccagata tggactacgg
    14881 gcaacaatat tgatggtatc gatttgcagc aaggacatgc gggagatcgt tgccggaata
    14941 tcctccaacg acgcttgacg gcggggtttg tttaacaggc cttgtgcggc aaggtgaaga
    15001 ttacgcgcat cagcaaggga gaggtgcggc agcgacattc atgactccat caatcgaacg
    15061 ctgccgcggc gtaactagtt gccagaagcc agcgaggtta gttgcgtaag cagtttcgct
    15121 ggttcatcac ctgaaagctg tgcgtctaca ggcaaatacc accaattttc ttctgcaaag
    15181 gcccggcatt tcaccgcatc tttttcagtc attaccagcg tttgcccggc gcttaccaac
    15241 gcactgacat ccgcatggtt caaagactga tgatcggcca gcggtacaca tttttccggt
    15301 tgtacgccac acatcttcag cgtggcaaaa aagcgcggcg gatgcccaat ccccgccatc
    15361 gccactacat gttcaagctg agcaacgtca caacgcgtac cggtacgtaa attcaccgcc
    15421 tgacccggca gcagatgcat ggggatttca ccgctgcgag ggacaccgcc gttgacgatt
    15481 accgcatcaa ccgactttaa gcgccccgct cgctcacgca ttggccccgc cggcaaccac
    15541 cagccattgc caaagcgacg cacaccatca ataacgacaa tttccacatc acgcgccaga
    15601 cggtaatgct gtaaaccgtc gtcggttacg atgatctgca catcagggtg ttgcgccaga
    15661 atggctttta ccgcatcaga acgaacggga gaaaccgcaa caggcgcatc agtgcgttga
    15721 taaatcaaca caggttcatc acccgcctgt gctgttgtgg tatctgccga caataacagc
    15781 ggataagatt cagccttacc accatatccc cgcgatacga cccccacgcg aataccgcgc
    15841 tgttgcaact gttccaccag ccagacaacg accggggttt ttccgttgcc gcctgcggtg
    15901 agattaccaa ccacgacaac cggtacgggg gcacgccagg cgcgcttcag ttttagttta
    15961 tagcaaagac ggatcgcgcc acttaccagg ccatacaacc aggagagtgg cagcaatagc
    16021 cgccacaaag gggattcacc agaccagatt ttttcgatca ttggccaaac tgcattttgt
    16081 gaagttgcgc gtaaacgccg cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa
    16141 tgacaccatc ctcgacgacc acgatttcgt ctgccttttc aatggtagac aagcggtggg
    16201 caatcaccag agaggtacgg tttttctgca actcatccag tgccgcctga atcgcacgtt
    16261 cggattcggt atccagagcc gaggtagctt catccagaat cagaatcggg ctatcacgca
    16321 gcaaggctcg agcgatagca atacgctgac gctgaccgcc agagagcagc acgccgtttt
    16381 caccaatcac tgtatcgaga ccgttatcca tcttattgat gaagtccatg gcgtaggcca
    16441 tacgcgccgc ttcttcaatt tgctcacggc tgtactgttc agtccgtgcg taagcaatgt
    16501 tgttagcaac cgtatcgtta aacagatgga cattctgcga caccagagca acctggttac
    16561 gtaacgacgc cagggtatac tcgcgcagat cgtgaccatc catcaggatt tcgccttcat
    16621 caatatcgta aaaacgagtg atcaggctgg cgatggttga tttacctgaa ccagagcgtc
    16681 caaccagagc aaccgtcttc cctgccggaa ttttcaggtt gatgttacgc aatgcaggta
    16741 cgtcacgtcc cggataagta aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct
    16801 cgatcacgcg cttaccttcg tctttctcct gctcactgtc cagaatggta aacagcgtct
    16861 gacaagccgc cataccgcgc tggaactggg cgttaacgtt ggtcagtgat ttcagcggac
    16921 gcatcagtgc aatcattgaa gagaaaacaa cggtaatcgt accggcagtc aggctatcca
    16981 tgacacttgg gaagctcgcc gcatacagaa caaacgccag cgccaaagag gcgatcagct
    17041 gaatgatcgg atcagagatg gaagaggctg aaaccatttt catcccctga agacgcattc
    17101 ggttgctgac tttatcaaag cgtttcgttt ccacttcctg accaccgaaa atcaatactt
    17161 ctttgtggcc cttcagcatt tgttcggcgc tggtggtcac ctgccccatg gtgttctgca
    17221 tgtttttact gatgttgcga aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg
    17281 gtgccagcac aatcaaaatg atcgacagtt gccaactgta atagaacatc atgatgaaca
    17341 ggccgatgat tgacgcacct tcacgcacaa ccgttatcag tgcgccggaa gaagaagaag
    17401 caacctgttc ggaatcgtag gtgatacgtg acaacagcgt ccccgttgac tgtttgtcaa
    17461 aaaatgaaac cggcattccc atcatgtgac caaacaggcg gcgacgcatg gtcattacca
    17521 cctttcctga tacccaggag atacagtagc tggagacata gctggtgata ccacgtaaaa
    17581 tcatcagccc gatcaccacc agcggcatcc acaccagcac ggagcgatct gttttaccaa
    17641 agccatcatc aagaagtggc ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga
    17701 ggattaacgc tacgcccgcc acgatcagac ccgctttaaa aggcgcaatg gttggccaca
    17761 gtcggcggaa cgtctgccac gtagagagat ctttgtcgtt atgcattcaa aaaaccagca
    17821 tttgttgaaa tagccgcata ttctacccgt tatccactgg cacgccaaac cactgatgat
    17881 accaacgagg taaaatttgc tctctgaggc tgctaatccg ccagccttgc gctgaaaaat
    17941 tgaccgtcac ttgaccctga tgtggagtat caagccattg atatccttgc tgttgatagc
    18001 gatgcttaac tttattagag ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg
    18061 ccacttttcc attcactcgc tgaattaatg gcaacgatga tgaggtatta ctgccatggt
    18121 gaggtacctg aagcaatgtt gtctgcactt gctgccagta acggcttagc atcttttgtt
    18181 cagcggggac ttcaatatca ccggttagaa gaatgctatt cgtcccgtca tcaaccttaa
    18241 ccacacagga atggttattt cctttatcgt tgctagcttg taaaggccag tgcacgctga
    18301 aacgcaatcc ttgccattgc cacgcttcgc cacgcacaca gggctgatga tgttcccagt
    18361 ttaacggact tctgatccat aacatcggcc atgtgtgcaa tattgagtcc agccctcccc
    18421 ggtgatccag atgttcatga ctcagaataa cgccttccgg ttcaagatta tgccagtgga
    18481 gccaggggat aatcagttgt tgcccactat ccccttcggg ccaggccagt cctgtgtcat
    18541 agagaatcgc tttgccgttt ctggctatca ccattgccag cccttgcccg acatcaagca
    18601 tgtacacctg ccactcgtca ggtcgaggtt tttgccacag cggccagcac atcagcaagc
    18661 ctacagccac acacattgct ggcaacgttc gccaggcgtt taatcgccat accaccagta
    18721 agaaccatgg ggaaaatgat agccattgcc aacgttcagc aatgttgatc cacccttctg
    18781 gcaaactctt taacccccag aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc
    18841 cttgctccag gattaacggc ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa
    18901 ccgtgataaa tgtcaccaag ggaattgcta atagatttgc aataaacgag gtcagactaa
    18961 tgccatgaaa tatgacgatt tgcacaggca taagcaggag tgtgattccc agttgcaaat
    19021 gaatgaggga aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc
    19081 actggtacca aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga
    19141 gaatggcaac aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc
    19201 cactccactg tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac
    19261 gcaatgcagg aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccac
    19321 caattaatgg catttgccag tggatccagc gcccaggcag ataaacttgc ccaccgcgaa
    19381 tgagtccggc agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat
    19441 gcgccgttcc agtatcacgc atgatatttt tgatttcttt ggggacggat aaccgttccc
    19501 ccatacctaa accaagaata accgcattcc acatataggg ttgcagcgtt gtttgtagtg
    19561 acgccagata ctgtgcacgc aggctacaat tcggttcaat gacacttgcc tgcagaaagc
    19621 ggccggtgag cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat
    19681 taagttggcc atgaactgca cgaactttga gtttcattga ccattgttgt ccagcacaaa
    19741 ccgcttgcgg aagatattcg ccatacagca caaggcctgg cgcagggaat atacgtcgac
    19801 cttgtagatg agtaatttga ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa
    19861 caattgcatc ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg
    19921 ataatatgcc ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag
    19981 ggataaatgc cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcccaggca
    20041 attggggcaa aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta
    20101 ttttcatgca cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt
    20161 gggaattttg cgttccccga ggcacattca gaagaattta acgaaagggt gaaaactgct
    20221 caaaactctg cgaggcagct tccagttcct gattagagaa atgaaaaaag cacccgacag
    20281 gtgcttttct cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg
    20341 cagttcttta ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc
    20401 agtcttcgga ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc
    20461 gcggatttca atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt
    20521 tactgcatct tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat
    20581 caattctgac ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt
    20641 gctgcattga ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg
    20701 caatctgtca agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta
    20761 ctcgccttta gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc
    20821 ctgtttgtta acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga
    20881 caggctgatt gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc
    20941 aacgctcaga accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag
    21001 gtaaccttca acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac
    21061 tttaccggtt acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc
    21121 tgcgagctgt ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac
    21181 tgcagcgatt tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca
    21241 ggagatgtca gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc
    21301 gaagtcagtg atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc
    21361 gaactgctgc cacgggttag ctttgcactg tttcagaccc agggagatac gacgacgttc
    21421 ttcgtcgata tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg
    21481 gtggatgttt ttgttggtcc aatccatttc ggaaacgtgt accaggcctt caacgccttc
    21541 ttcgatttca acgaagcagc cgtagtcggt caggttggtc acgcgaccag tcagtttggt
    21601 accttccgga taacgtttag cgatagctac ccacggatct tcgcccaact gtttcaggcc
    21661 cagggataca cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc
    21721 cacgttgacg atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag
    21781 caggccgtca acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat
    21841 acctttaact tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt
    21901 ttcggattcg ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt
    21961 gattacttta aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac
    22021 gtctaccaga gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc
    22081 cttaactttg ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag
    22141 cgtgatccag gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc
    22201 gtcttctact gcgtccagag caacgtcaac ttcatcacct acctggattt ccagctcgcc
    22261 ctgggcgttt ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac
    22321 cagtactacg tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt
    22381 ttcgatttct tttaaggagt cttcaaagag ttgagcaaaa gattcagtca tgtttaatct
    22441 tcaggtttat atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg
    22501 ctgtcattcc attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc
    22561 tggcgcgcgt attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc
    22621 aacactaaag catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg
    22681 tcgcgttctt tgatctcggc caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc
    22741 aactgtagca tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact
    22801 ggcgcatcag ggaataccac cgttcccatg tcgcggccat cggcaatcag gccgggtaat
    22861 tcgcgaaacg cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact
    22921 tgtgaagctg cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg
    22981 aggatcactt ccagattgcc attggttgac acaaaacgta catccagatg ggatgccagc
    23041 ggtaccagcg catcttctga cgcaacatca acatggtgat gtaatgccgc caatgccagt
    23101 acgcgataaa ttgcacccga atccagcaga tgccattgca atgcttccgc catagcctta
    23161 cacaaggtgc ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc
    23221 gtcatcttta tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc
    23281 cgacgccaca aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg
    23341 atttattgcg caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta
    23401 cttaccagag gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga
    23461 gtcaaacatg gactgggtgc gaccaccatc cagactagcc agtttttcaa agctgccaac
    23521 cagcccctgg gtgctgatgc cacgtttctt caacagatca aaggagaaat catctgcttc
    23581 ggactcttta tcacgggagt atttagcatt aatagcgcct tctgcgatat cacccaattg
    23641 tgagcgggaa agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac
    23701 gatcgcataa gaagctttca tttcagccag cgagtgaccc aacgcgacgt ggcccagttc
    23761 atggcccaga acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac
    23821 acggacacag ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata
    23881 gttgaccggc gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt
    23941 accgtacttg ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg
    24001 attggcaatc gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc
    24061 ggagctgata gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc
    24121 tgcagaggtc gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt
    24181 aatcatatat aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc
    24241 aaaatgactc acaaggtccg aaaaaaaacg ccgacaatgt gccgacgttt tgataaagaa
    24301 agatttggct atttattgcc cgttgttcat tcaggctgcc tggctaatcc gcgccaactg
    24361 ctcgaaataa tccggaaatg ttttggccgt acatttggga tcaagaatcg tcactggtgt
    24421 atctgacaac gccaccagcg agaaacacat cgccatccgg tgatcattgt acgtcgcgat
    24481 ctcggcaaag ttcagttttt ccggtggagt gatacgaatg taatcgtgcc cctcttctac
    24541 ttccgcaccg actttacgca gttctgttgc catcgcaaac aggcgatcgg tttctttaac
    24601 acgccagtta tagatattgc gcagcgtggt ggtgcctttt gcaaataacg ccgccgtggc
    24661 aatggtcatc gccgcatcgg gaatatggtt catatccata tcaatagcgt tcagttcacc
    24721 acgcgtgcag gaaatataat catcgcccca gcaaatggtc gcgcccattt tttccagcac
    24781 atcagcaaag cgaatatcac cctgcatact gttacgtcca ataccggtca cttttacagt
    24841 gccgcctttg attgctgctg ctgccagaaa gtaagaagcc gaagatgcat cgccttcgac
    24901 caaataagta cccggagact gataagactg cccgcctttt acgacaaatt gttgatagtg
    24961 ctgattttca atttcaacac caaacgtctt catcagattg agtgtgatgt cgatataagg
    25021 tttagaaacc agatcgcctt taatacgaat caccgtatct tccggcgcaa gaggcgcagt
    25081 cattaacagt gcggtgagga attggctgga aacggagcca tcaacgtcaa cgttgccgcc
    25141 agtaaagccg ccctgtaaac gcaacggcgg ataattttct tgttccaggt aagtgatctt
    25201 cgccccgccc aggcgcagcg catccaccag atgaccaatc gggcgttctt tcatacgcgg
    25261 ctcaccggtc agcacaatat cattgctacc cagacaaaga gctgccgcca gcggacgcat
    25321 tgccgttccg gcgttaccga ggaacaactc cagggcacct tctgcgtgta atggaccgcc
    25381 gttaccgata atttcgcaac gcgtacgatc ggctgaaagc gtatagctta cccctaacgc
    25441 tgttaatgca ttcagcatat ggcgcacgtc atcgctatcc agcagattgg ttaatactgt
    25501 tttgccgtgt gctaatgccg ccagcaataa agcgcggtta gaaacgctct tggaaccggg
    25561 cagattaata gtgccatcga cacgagcgat gggttgtaac gtcagggatt ccatgaactc
    25621 aactctctac aacagaaata aaaaccccac aggctggctg tggggattaa gcaaaatttc
    25681 ggcattaacc gtgacggcgt tcgaactcaa ccatgaagtc tgtcagcgct ttaacgcctt
    25741 ccagcggcat ggcgttataa atagaagcgc gcattccgcc gaccacacgg tgacctttca
    25801 gtgcatgaag gccagcagca aaagactctt caaggaacaa tttgtcaagc gcactgtccg
    25861 ccaactggaa cggcacgttc atccgcgaac ggttagcttt cgccacgtca ttgcggtaga
    25921 aatcgctgtt atcaatcacc ccatatagca gttctgcttt ttgctgattg attttatcca
    25981 tttcagctac accgccgttc gctttcagcc atttaaagac cagaccagat agataccagg
    26041 caaatgtcgg cggcgtgtta aacatggagc cgttatcgtt gaggatggaa taatccagaa
    26101 tcgacggaca cgcgatattc gctttgccca gcaaatcttc acgaacgatg acgattgtca
    26161 ggccagccgg gccgatattt ttctgcgcgc cagcgtaaat cacgccataa cggctgacgt
    26221 caatcggacg ggaaagaatg gttgaagaga agtcggcggc gaccaccaca tctttgccga
    26281 agtctggcgt ttcgtcgatg gcgataccgt cgatggtttc attcgggcag taatgcatat
    26341 aagcagcatt atcagagagt tgccattcac gcattggctt aaccgcgcgc agaccatcaa
    26401 cagtcacttt ggcgtcaaag acattaggcg tgcagtattt tttcgcttct ttaatggcac
    26461 ttgccgccca gtaaccggca tcaacataat ctgcggtggt tttatcaccg agaatattca
    26521 gcggtaccgc agcaaactga ccgcgaccac cgccatggca gaataatacc ttgtagttgg
    26581 aggggacatt aagaagatcg cgaaaatcct tctcggcttc ctctgcaacc tgaatgaact
    26641 ctttgccacg gtgactcact tccatcaccg acgtaccaag accgttccag tcgcgcagtt
    26701 cctgttgagc ctgtttaagc acctctgccg gtagcattgc cggaccagaa ctaaaattga
    26761 agatttgagc catttcccct caccacgttg cgttgctatt aacccgctgt caatcgattg
    26821 cgaccacagg taatacatta ttcctgtggc tatcggtttt atcattcagt gacacacatc
    26881 gcaatgagta aaaccgttga gggcaaaaat gtggcttgca tcacacaaaa gaatctctcg
    26941 gattattagc aaaaagccga tgtttttact gtcaggtcac ccgttttgcc cgacttgcct
    27001 tcgtccattc tggatttgca cagcgcggac ataattgttt ttcccccgct ttcatatgaa
    27061 tgatttcact acaacgaata caggcatagt ccgccgactc cggcaccact ttgtaacgct
    27121 gcgtacaatc actgggtaaa ataagtaaac ccggtttcac cttgtcatct tcaaagaaat
    27181 agacactgat ttgcccgttg gtttcgagaa tcgccagacg tacctgcccc agctgctcca
    27241 cgccacgcaa tcgtagctcc ataaagaact caaattccgt catgttggag ttattgagtt
    27301 tcgaccaggc cagctcgcca tcttcaataa taacaaccgg cttaccttcc agtagatctt
    27361 ccagtttttc actgtgcgcc atcaaccaca ttaccaggcg gtataacaac gccagagtga
    27421 taaagacgat aagtaccggc accatcggca catcatcata aaacgccaca tctcccgccg
    27481 ctgaacccag cgtgagaatg attaacactt caaacagcga catctgtcgc acaccgcgtc
    27541 ttccggtcat tttgaggaac aaaaaaacca gtacaaaggt ataaagacta cgtagtgcaa
    27601 cttcgccaag gaaatcaaaa ggcactttat caaatgccat acggtggaga tcgaacgcct
    27661 tcatttttat gctcttatcg tggtttatcc gctaaaagca tagtcaacag ttcatcggca
    27721 tggcggcggt gatagcgcct ttgagataaa acccgtatta ttgcgcgctt tccgtacgac
    27781 taaagtgatt ttcatgacgc aaacatttat ccccggcaaa gatgccgctc tggaagattc
    27841 catcgctcgc ttccagcaaa aactttcaga cctcggcttt cagattgaag aggcctcctg
    27901 gctgaatcct gtgcctaacg tctggtctgt acatattcgc gacaaagagt gcgcactgtg
    27961 ttttaccaac ggaaaaggcg caaccaaaaa agcggcgctg gcttctgcac ttggtgaata
    28021 tttcgagcgt ctctcaacca actacttttt tgctgatttc tggctgggcg aaaccatcgc
    28081 caacggtccg ttcgtgcatt atcccaacga aaaatggttc ccactgaccg aaaatgacga
    28141 tgtgccagaa ggactactcg atgaccgtct gcgcgcgttt tatgatccgg agaatgaact
    28201 gaccggcagc atgctgattg acctacaatc cggtaacgaa gatcgtggta tttgcggcct
    28261 gccgtttacg cgtcagtccg acaatcagac cgtttatatt ccgatgaata tcattggtaa
    28321 cctgtacgtc tccaacggta tgtccgcagg taatacccgc aacgaagcac gcgttcaggg
    28381 attgtctgaa gttttcgaac gctacgtgaa aaaccgcatt attgctgaaa gcatcagcct
    28441 gccagagatc ccggcagacg tgctggcgcg ttacccagca gtagttgaag ccatcgaaac
    28501 gctggaagcg gagggtttcc caatcttcgc ttatgacggt tcgcttggcg gccagtatcc
    28561 ggtgatttgc gtggtactgt tcaatcctgc taacggtacc tgctttgcct ctttcggtgc
    28621 gcatcctgat tttggcgtag cactggaacg taccgtgacc gagctgctgc aaggtcgtgg
    28681 cctgaaagat ttggatgtgt ttactccgcc aaccttcgat gatgaagaag tcgctgaaca
    28741 taccaacctc gaaacgcact ttatcgattc cagcggttta atctcctggg acctgttcaa
    28801 gcaggatgcc gattatccgt ttgtggactg gaatttctcc ggcaccacgg aagaagagtt
    28861 tgctacgctg atggctatct tcaacaaaga agataaagaa gtttatattg ccgattacga
    28921 gcatctgggc gtttatgctt gccgtattat cgtgcctggc atgtccgata tttatccggc
    28981 tgaagatctg tggctcgcga ataacagtat gggcagccat ttacgtgaaa cgattctttc
    29041 gctaccaggc agcgagtggg aaaaagaaga ttacctgaac ctcatcgagc aactggatga
    29101 agaaggtttt gatgacttta cccgcgtgcg tgagctgttg ggtctggcga ccgggtcgga
    29161 taacggttgg tacaccctgc gtatcggtga attaaaagcc atgctggcgc tggctggtgg
    29221 cgatctggaa caggctctgg tctggaccga atggacgatg gagtttaact catcagtatt
    29281 tagtccggaa cgcgccaact attatcgctg cctgcaaacg ttgttattac tggcacagga
    29341 agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt cgcatgtacg gcgcagatgc
    29401 cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg ttttacggcc tgcaaccggt
    29461 agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg ttgaaggcct acgaaaagct
    29521 gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa atagcctacg caatgtaggc
    29581 ttaatgatta gtctgagtta tattacgggg cgttttttta atgcc
//
LOCUS       DAZFWL020000048        29230 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFWL020000048 DAZFWL020000000
VERSION     DAZFWL020000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.048"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            97..1050
                     /gene="ybhD"
                     /locus_tag="ACHKNP_RS11405"
                     /old_locus_tag="pgaptmp_002281"
     CDS             97..1050
                     /gene="ybhD"
                     /locus_tag="ACHKNP_RS11405"
                     /old_locus_tag="pgaptmp_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4704444.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1091..2086)
                     /gene="pgl"
                     /locus_tag="ACHKNP_RS11410"
                     /old_locus_tag="pgaptmp_002282"
     CDS             complement(1091..2086)
                     /gene="pgl"
                     /locus_tag="ACHKNP_RS11410"
                     /old_locus_tag="pgaptmp_002282"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HGU4704445.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2241..3059
                     /gene="ybhA"
                     /locus_tag="ACHKNP_RS11415"
                     /old_locus_tag="pgaptmp_002283"
     CDS             2241..3059
                     /gene="ybhA"
                     /locus_tag="ACHKNP_RS11415"
                     /old_locus_tag="pgaptmp_002283"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HGU4704446.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3060..4118)
                     /gene="modC"
                     /locus_tag="ACHKNP_RS11420"
                     /old_locus_tag="pgaptmp_002284"
     CDS             complement(3060..4118)
                     /gene="modC"
                     /locus_tag="ACHKNP_RS11420"
                     /old_locus_tag="pgaptmp_002284"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HGU4704447.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4121..4810)
                     /gene="modB"
                     /locus_tag="ACHKNP_RS11425"
                     /old_locus_tag="pgaptmp_002285"
     CDS             complement(4121..4810)
                     /gene="modB"
                     /locus_tag="ACHKNP_RS11425"
                     /old_locus_tag="pgaptmp_002285"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HGU4704448.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4810..5583)
                     /gene="modA"
                     /locus_tag="ACHKNP_RS11430"
                     /old_locus_tag="pgaptmp_002286"
     CDS             complement(4810..5583)
                     /gene="modA"
                     /locus_tag="ACHKNP_RS11430"
                     /old_locus_tag="pgaptmp_002286"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4704449.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEEHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5749..5898)
                     /gene="acrZ"
                     /locus_tag="ACHKNP_RS11435"
                     /old_locus_tag="pgaptmp_002287"
     CDS             complement(5749..5898)
                     /gene="acrZ"
                     /locus_tag="ACHKNP_RS11435"
                     /old_locus_tag="pgaptmp_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HGU4704450.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            6027..6815
                     /gene="modE"
                     /locus_tag="ACHKNP_RS11440"
                     /old_locus_tag="pgaptmp_002288"
     CDS             6027..6815
                     /gene="modE"
                     /locus_tag="ACHKNP_RS11440"
                     /old_locus_tag="pgaptmp_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HGU4704451.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6883..8355
                     /gene="modF"
                     /locus_tag="ACHKNP_RS11445"
                     /old_locus_tag="pgaptmp_002289"
     CDS             6883..8355
                     /gene="modF"
                     /locus_tag="ACHKNP_RS11445"
                     /old_locus_tag="pgaptmp_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HGU4704452.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            8616..9632
                     /gene="galE"
                     /locus_tag="ACHKNP_RS11450"
                     /old_locus_tag="pgaptmp_002290"
     CDS             8616..9632
                     /gene="galE"
                     /locus_tag="ACHKNP_RS11450"
                     /old_locus_tag="pgaptmp_002290"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGU4704453.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9642..10688
                     /gene="galT"
                     /locus_tag="ACHKNP_RS11455"
                     /old_locus_tag="pgaptmp_002291"
     CDS             9642..10688
                     /gene="galT"
                     /locus_tag="ACHKNP_RS11455"
                     /old_locus_tag="pgaptmp_002291"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGU4704454.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10692..11840
                     /gene="galK"
                     /locus_tag="ACHKNP_RS11460"
                     /old_locus_tag="pgaptmp_002292"
     CDS             10692..11840
                     /gene="galK"
                     /locus_tag="ACHKNP_RS11460"
                     /old_locus_tag="pgaptmp_002292"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGU4704455.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYYLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11834..12874
                     /gene="galM"
                     /locus_tag="ACHKNP_RS11465"
                     /old_locus_tag="pgaptmp_002293"
     CDS             11834..12874
                     /gene="galM"
                     /locus_tag="ACHKNP_RS11465"
                     /old_locus_tag="pgaptmp_002293"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HGU4704456.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            13077..13829
                     /gene="gpmA"
                     /locus_tag="ACHKNP_RS11470"
                     /old_locus_tag="pgaptmp_002294"
     CDS             13077..13829
                     /gene="gpmA"
                     /locus_tag="ACHKNP_RS11470"
                     /old_locus_tag="pgaptmp_002294"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HGU4704457.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13988..15040)
                     /gene="aroG"
                     /locus_tag="ACHKNP_RS11475"
                     /old_locus_tag="pgaptmp_002295"
     CDS             complement(13988..15040)
                     /gene="aroG"
                     /locus_tag="ACHKNP_RS11475"
                     /old_locus_tag="pgaptmp_002295"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HGU4704458.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGETAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15356..15736
                     /gene="ybgS"
                     /locus_tag="ACHKNP_RS11480"
                     /old_locus_tag="pgaptmp_002296"
     CDS             15356..15736
                     /gene="ybgS"
                     /locus_tag="ACHKNP_RS11480"
                     /old_locus_tag="pgaptmp_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HGU4704459.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15850..16791
                     /gene="zitB"
                     /locus_tag="ACHKNP_RS11485"
                     /old_locus_tag="pgaptmp_002297"
     CDS             15850..16791
                     /gene="zitB"
                     /locus_tag="ACHKNP_RS11485"
                     /old_locus_tag="pgaptmp_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HGU4704460.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16788..17507)
                     /gene="pnuC"
                     /locus_tag="ACHKNP_RS11490"
                     /old_locus_tag="pgaptmp_002298"
     CDS             complement(16788..17507)
                     /gene="pnuC"
                     /locus_tag="ACHKNP_RS11490"
                     /old_locus_tag="pgaptmp_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGU4704461.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRLAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17545..18588)
                     /gene="nadA"
                     /locus_tag="ACHKNP_RS11495"
                     /old_locus_tag="pgaptmp_002299"
     CDS             complement(17545..18588)
                     /gene="nadA"
                     /locus_tag="ACHKNP_RS11495"
                     /old_locus_tag="pgaptmp_002299"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HGU4704462.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18837..18961)
                     /locus_tag="ACHKNP_RS11500"
                     /old_locus_tag="pgaptmp_002300"
     ncRNA           complement(18837..18961)
                     /ncRNA_class="other"
                     /locus_tag="ACHKNP_RS11500"
                     /old_locus_tag="pgaptmp_002300"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(18993..19068)
                     /locus_tag="ACHKNP_RS11505"
                     /old_locus_tag="pgaptmp_002301"
     tRNA            complement(18993..19068)
                     /locus_tag="ACHKNP_RS11505"
                     /old_locus_tag="pgaptmp_002301"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19033..19035),aa:Lys,seq:ttt)
     gene            complement(19102..19177)
                     /locus_tag="ACHKNP_RS11510"
                     /old_locus_tag="pgaptmp_002302"
     tRNA            complement(19102..19177)
                     /locus_tag="ACHKNP_RS11510"
                     /old_locus_tag="pgaptmp_002302"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19142..19144),aa:Lys,seq:ttt)
     gene            complement(19181..19256)
                     /locus_tag="ACHKNP_RS11515"
                     /old_locus_tag="pgaptmp_002303"
     tRNA            complement(19181..19256)
                     /locus_tag="ACHKNP_RS11515"
                     /old_locus_tag="pgaptmp_002303"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19221..19223),aa:Val,seq:tac)
     gene            complement(19406..19481)
                     /locus_tag="ACHKNP_RS11520"
                     /old_locus_tag="pgaptmp_002304"
     tRNA            complement(19406..19481)
                     /locus_tag="ACHKNP_RS11520"
                     /old_locus_tag="pgaptmp_002304"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19446..19448),aa:Lys,seq:ttt)
     gene            complement(19484..19559)
                     /locus_tag="ACHKNP_RS11525"
                     /old_locus_tag="pgaptmp_002305"
     tRNA            complement(19484..19559)
                     /locus_tag="ACHKNP_RS11525"
                     /old_locus_tag="pgaptmp_002305"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19524..19526),aa:Val,seq:tac)
     gene            complement(19695..19770)
                     /locus_tag="ACHKNP_RS11530"
                     /old_locus_tag="pgaptmp_002306"
     tRNA            complement(19695..19770)
                     /locus_tag="ACHKNP_RS11530"
                     /old_locus_tag="pgaptmp_002306"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19735..19737),aa:Lys,seq:ttt)
     gene            complement(19935..20726)
                     /gene="cpoB"
                     /locus_tag="ACHKNP_RS11535"
                     /old_locus_tag="pgaptmp_002307"
     CDS             complement(19935..20726)
                     /gene="cpoB"
                     /locus_tag="ACHKNP_RS11535"
                     /old_locus_tag="pgaptmp_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HGU4704463.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20736..21257)
                     /gene="pal"
                     /locus_tag="ACHKNP_RS11540"
                     /old_locus_tag="pgaptmp_002308"
     CDS             complement(20736..21257)
                     /gene="pal"
                     /locus_tag="ACHKNP_RS11540"
                     /old_locus_tag="pgaptmp_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HGU4704464.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(21292..22584)
                     /gene="tolB"
                     /locus_tag="ACHKNP_RS11545"
                     /old_locus_tag="pgaptmp_002309"
     CDS             complement(21292..22584)
                     /gene="tolB"
                     /locus_tag="ACHKNP_RS11545"
                     /old_locus_tag="pgaptmp_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HGU4704465.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22717..23982)
                     /gene="tolA"
                     /locus_tag="ACHKNP_RS11550"
                     /old_locus_tag="pgaptmp_002310"
     CDS             complement(22717..23982)
                     /gene="tolA"
                     /locus_tag="ACHKNP_RS11550"
                     /old_locus_tag="pgaptmp_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HGU4704466.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(24047..24475)
                     /gene="tolR"
                     /locus_tag="ACHKNP_RS11555"
                     /old_locus_tag="pgaptmp_002311"
     CDS             complement(24047..24475)
                     /gene="tolR"
                     /locus_tag="ACHKNP_RS11555"
                     /old_locus_tag="pgaptmp_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HGU4704467.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24479..25171)
                     /gene="tolQ"
                     /locus_tag="ACHKNP_RS11560"
                     /old_locus_tag="pgaptmp_002312"
     CDS             complement(24479..25171)
                     /gene="tolQ"
                     /locus_tag="ACHKNP_RS11560"
                     /old_locus_tag="pgaptmp_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HGU4704468.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(25168..25572)
                     /gene="ybgC"
                     /locus_tag="ACHKNP_RS11565"
                     /old_locus_tag="pgaptmp_002313"
     CDS             complement(25168..25572)
                     /gene="ybgC"
                     /locus_tag="ACHKNP_RS11565"
                     /old_locus_tag="pgaptmp_002313"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU4704469.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25722..26015)
                     /gene="ybgE"
                     /locus_tag="ACHKNP_RS11570"
                     /old_locus_tag="pgaptmp_002314"
     CDS             complement(25722..26015)
                     /gene="ybgE"
                     /locus_tag="ACHKNP_RS11570"
                     /old_locus_tag="pgaptmp_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HGU4704470.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(26015..26128)
                     /gene="cydX"
                     /locus_tag="ACHKNP_RS11575"
                     /old_locus_tag="pgaptmp_002315"
     CDS             complement(26015..26128)
                     /gene="cydX"
                     /locus_tag="ACHKNP_RS11575"
                     /old_locus_tag="pgaptmp_002315"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HGU4704471.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(26143..27282)
                     /gene="cydB"
                     /locus_tag="ACHKNP_RS11580"
                     /old_locus_tag="pgaptmp_002316"
     CDS             complement(26143..27282)
                     /gene="cydB"
                     /locus_tag="ACHKNP_RS11580"
                     /old_locus_tag="pgaptmp_002316"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU4704472.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(27298..28866)
                     /gene="cydA"
                     /locus_tag="ACHKNP_RS11585"
                     /old_locus_tag="pgaptmp_002317"
     CDS             complement(27298..28866)
                     /gene="cydA"
                     /locus_tag="ACHKNP_RS11585"
                     /old_locus_tag="pgaptmp_002317"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HGU4704473.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7275 a         7736 c         7188 g         7031 t
ORIGIN      
        1 aataacattt tttcattaat ttaagttggc taaatgcagt taaaattttt aacgaccagc
       61 cacccaaaat cgtcgcttcc attttccgga gtaataatga agcatgaatt atcaagtatg
      121 aaggcatttg tcatactggc agagtccagt tcatttaaca atgctgctaa attactcaat
      181 attacgcaac ctgcattaac gcgaagaata aaaaagatgg aggaagattt acatattcaa
      241 ctatttgagc gtacaactcg caaggttacg ttaacaaaag caggaaaaag gttgctcccg
      301 gaggcgcggg aattaataaa gaaattcgat gaaacgcttt ttaatattcg tgatatgaat
      361 gcttatcatc gtggtatggt gacgttagcg tgtattccga ccgcagtatt ttatttttta
      421 ccgctggcaa tcggtaaatt taatgagctg tatcccaata ttaaagtgcg gattctggaa
      481 caaggcacga ataattgcat ggaatcggtg ctgtgtaacg aatcagactt tggcataaac
      541 atgaacaacg tcacaaattc atccattgat tttaccccac tggtcaacga gccattcgtg
      601 ctggcctgtc ggcgtgacca tccattggcc aaaaagcagc ttgtagaatg gcaggagtta
      661 gtgggttaca aaatgattgg cgtacgttcc tccagcggca accgattgtt gatagaacaa
      721 cagctagccg acaagccctg gaagctggac tggttttacg aagtgcgtca tctttcgacg
      781 tcattaggac tggtagaggc gggactgggg atttcggcgc ttcctggtct ggcaatgccc
      841 catgcgccct attcttcgat tattggtatt ccactggtgg aacccgttat acgtcggaca
      901 ttggggatta ttcgccgcaa agatgccgtg ctttctccgg cagcagaacg cttttttgcc
      961 ttgctgatta atttgtggac tgacgataaa gacaatttgt ggaccaatat cgtcgaacgc
     1021 cagagacatg cgttacagga gataggttaa tacaccggcg caggagagac tgcgccggtt
     1081 aaatcagcgg ttagtgtgcg ttaaccacca cccacattgg tccctgcccg accgcataac
     1141 ggcctttttc atgcagtagt ccctgctcgc caacaatttc gtataccgag atgtggtgag
     1201 atttttgccc ggcggcaatc agatacttgc cgctgtgatc aacattgaag ccgcgcggct
     1261 gggtttccgt tggctggaag ccttctttac tcaacacgct gccatcttcc gaaacgctga
     1321 aaacggtaat caggctggcg gtacggtcgc aggcgtataa atggcgacca tccggggtga
     1381 tatgaatatc agccgcccaa cgggtgtcgg agaagttttc cggcatcata tccagcgtct
     1441 ggacacattc gatattaccg tgcggatctt tcagttccca gacatccact gagctgttta
     1501 actcattgac gcaatacgca tattgttcgt ttggatggaa taccatatga cgcgggccgg
     1561 ccccttcaac ggtggtcact tccgcagggt cctgcgccac gagatgacca tcatcgctga
     1621 ccgtaaacag gcaaatgcga tcctgcttta atgccggaac ccacagcgta cggttgtccg
     1681 gtgagatatt ggcggaatgg caaccgtcca gcccctcgac cacatcgacg acgcccactg
     1741 gcaggccatc ttccagacgc gttacgctca cgttacccgc attgtaagaa cctacaaaga
     1801 caaactgccc ctggtgatcg gtggaaatat gcgtcggact acccggcagc gcagactctg
     1861 cggcaaaggt cagtgcgcca tcgtccgggg cgatactata cgccaggacg cgaaactcag
     1921 ggcgaacacc aacatagaga taacgtttgt ccgggctgac caccatcggc tgcacctgcc
     1981 ccggcacatc gacaacctgt gtcagcgtca gtgcgccttc atgattcagg ttccagacgt
     2041 gaatttgctg gctctcaggg ctggcgatat aaactgtttg cttcatgaat gctcctttgc
     2101 atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat gctgaattaa
     2161 aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg aaaattctta
     2221 accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta gacggcacct
     2281 tattgacccc gaaaaagacc ctgcttcctt catcgataga agccctggcc cgcgctcgcg
     2341 aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt catccttttt
     2401 atcaggcgct ggcgctggat acacctgcta tttgctgtaa tggcacctat ttgtatgatt
     2461 atcatgcaaa aaccgtgctg gaagcggacc caatgcccgt taataaagcc ctgcaactca
     2521 ttgagatgct gaatgaacac cacattcacg gtctgatgta tgtggatgat gcaatggtct
     2581 atgagcaccc gaccgggcat gtcattcgca cgtctaactg ggcgcaaacc ctgccgccgg
     2641 aacagcgtcc gactttcaca caagtcgctt ctctggctga aacggcgcaa caagttaacg
     2701 ccgtatggaa gttcgccctg acgcacgatg acctgccgca attgcagcat tttggtaagc
     2761 atgtcgaaca tgaactggga ctggagtgtg aatggtcctg gcacgatcag gttgatattg
     2821 cacgcggcgg caacagcaaa ggtaaacgtt tgacgaaatg ggttgaggcg caaggctggt
     2881 cgatggaaaa cgtcgtggcg ttcggcgata actttaatga tatcagtatg ctggaggccg
     2941 ctggtacagg cgtggcgatg ggcaacgccg atgacgcggt aaaagcgcgc gccaacattg
     3001 tgattggtga taacaccacc gacagcattg cccagttcat ttatagccac ctgatttaat
     3061 caggcggtta tcgacacact tttaatttgc gcgtacagcc acaggccagg tttgatcacc
     3121 agttcatccc tggcccacgg gctgatacgc gcccacagcg ttttacctcc gacttccagt
     3181 tccacttcca cctggccgtt gtcgtcataa ctattaacaa cttttgcccg caatacgtta
     3241 cgaatgctgg tttgctgcgg cggttgtaaa accagagaaa catcggaagc ctgaatgcgg
     3301 atacgtagcg cagcttgcag cggttcgtcc agcttattga cccacaaatg ctgatcgccc
     3361 agcgccagcg cagtcatcgc gtaatgcgga tgatgctcaa gtaccgtcac tttcagaatg
     3421 ctactttgtt gctctttcgg cagccacgga ttcatcacgc tactacccca gacttcttcc
     3481 agtgcgccaa aggctttcac ctgaccgttt tccagtacca tcactctgtc tgccagatgg
     3541 aggatctcat ccagcgaatg gctgacatac aacatcggga tgttgatttc ccgcgtcaga
     3601 cgttgcagat aaggcaacag ttcgcgttta cgcggaatat ccagtgacgc cagcggttca
     3661 tccagcaaca gcaattctgg tgccgtcagc aaagcccgcc caatcgctac gcgctgtttt
     3721 tcgcctccgg acaggctgcc tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc
     3781 accagcttat cgaactgatc gaccatgctt ttcgccatgc cgtagcgcag attgccacgc
     3841 actttgtaat gcggaaacag ccgcgcatcc tgaaaaacat agccaacgcg acgcttttct
     3901 ggcgtcaggc agataccttt ttcggcatca tttagtaccc gaccattgag gacaatcctc
     3961 cctttttgcg ggcgcgtcag tccactgatg gcgttaatca gcgaagtttt tccggcaccg
     4021 gagacgccaa agatagcagt gatgccattg gcgggcagcg tttcattaat ggtcaggcaa
     4081 tggttgccca aggtctggga aaaattcagt tccagcatga ttagcgcccc gcccgttcac
     4141 ggctgattct ggccagccat tctgaaatca acagggagat catcgccagc gcaatagaaa
     4201 taatgcacag tctcgccgcg ccactttccc cgccgggggt ctggatcagg gtatacatgg
     4261 cagaaggaat ggttcgcgtt tcaccaggaa tgttcgacac aaaggtgatg gttgcaccaa
     4321 actcaccgag agaacgagca aaagccagta ccgtaccaac aataattccc ggtaaggtca
     4381 gcggtaacgt gatagtaaag aaaacgcgcc agcgcccggc ccccagtgtt cttgcggcct
     4441 gttcgagttt gacatcaacc ccttccagcg ccagacgaat tgcccgcacc atcagcggaa
     4501 acgacatgac ggcggcagcg agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa
     4561 accagtcata cagacgttca ccgataaatc cgcgccgtcc catcgaaact aataataagt
     4621 aaccgacgac cacgggcggt aacaccagcg gtagatgcag tacgctgtcg agcagagctt
     4681 tgcccggaaa cgtgcaacgc accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa
     4741 acagcacagc cagggaagaa actttcaggc ttaataaaac tgcctgccat tctggatcgg
     4801 tcagtatcat tacttggttg taaatccgta acgtttaaag atttccgcag cctgcggccc
     4861 tttcaaataa tcataaaatg ctttcactgt cgcattgtta tgctcttcca caacagcaac
     4921 cggatattcc acttttttat gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt
     4981 gctggcaact gcgtcagaac cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa
     5041 cgccagcgcg ccacgaacat cttccgccgg agccagtttc ggggagagcg tatcccatgc
     5101 gcccagtttt tgcagtgctt ctttcgcata aatgccagca gggacatgtt ccggatcgcc
     5161 aaccgcgagg cgaccgccat tcagcagcga agtccagttg gttttgctgt cgatggtgaa
     5221 atctttctgc tcgctggctt tcggtgctac aacgaccagg ctattgccga gcagtgtctg
     5281 gcgcgaagcc gtatcgatcg cttttttatc aaccgcataa tccatccatt tctgatcggc
     5341 agagataaac agatccgcag gcgctcccgc ttcaatctga cgggccagag tagatgacga
     5401 agcgaaagaa gaaaccacat ccacgccttt ttctttttta tactgcgtag caatgtcctg
     5461 cattgcgtta gtcagtgatg cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt
     5521 attgccagca acagcgaaag agagtgccgc cccggcaaac aagttcaacc atttacgagc
     5581 cattggtaac cccttaatgt aacgttatgt aggcgacaat ataacggcta gctcagaatt
     5641 ttcccagcag ttattgctaa cctttgtgag gtagataaga aaaaatatcg gctggaaaag
     5701 caggaagttg agaaaaagaa atgcccgact aagcgggcat tcagggaatc aatgattttg
     5761 tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac caagaccgta
     5821 aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga ataccagact
     5881 ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc taaccgctaa
     5941 gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac
     6001 aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc
     6061 aacaaaaatt attcgccgac ccgcgccgga tttcgctact aaaacacatt gcgctttccg
     6121 gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca
     6181 ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag
     6241 gtggcggcgg cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac
     6301 tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc ctgccgctga
     6361 acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt
     6421 tcggcaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg
     6481 ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgcg cgtctggggc
     6541 tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggt attactcagg
     6601 acgaggcggt cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc
     6661 gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca
     6721 cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg gcctacttta
     6781 atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac
     6841 acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt
     6901 ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta
     6961 aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc
     7021 cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac
     7081 atcactcgtc tctccttcga gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat
     7141 aacaccgata tgctcggccc tggcgaagat gacaccggac gcactacggc tgagatcatt
     7201 caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc
     7261 gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg
     7321 tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg
     7381 gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt
     7441 actctggtac tggtgctcaa tcgcttcgat gagatcccgg agtttgtcca gtttgctggc
     7501 gtgctggcgg attgcacgtt agcggaaact ggcgctaaag aggaactgct ccaacaagca
     7561 ctcgtcgcgc aactggcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat
     7621 gaaccttcag cacgtcacgc cttacccgcc aacgaaccgc gcattgtgct gaacaatggc
     7681 gtggtttctt ataacgatcg ccccattctt aataacctta gctggcaggt gaatccaggt
     7741 gaacactggc aaattgtcgg gccaaatggt gcaggaaaat cgacgttatt aagcctggtt
     7801 actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggacg acgtcgcggc
     7861 agcggcgaaa ccatctggga tatcaaaaag catatcggtt acgtcagcag tagtttgcat
     7921 ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg
     7981 attggcattt atcaggccgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat
     8041 attctcggca ttgataaacg tacggctgac gctccgttcc atagtctttc ctggggacag
     8101 cagcgtctgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat
     8161 gaaccactac aggggcttga tccgctcaat cgccagctta tccgccgttt tgttgatgtg
     8221 ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct
     8281 gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg
     8341 acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag cgccctctcc
     8401 ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa
     8461 tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct
     8521 tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat
     8581 ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt accggtggta
     8641 gcggttacat tggaagtcat acctgtgtgc aattactgca aaacggtcat gatgtcatca
     8701 ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg
     8761 gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatac
     8821 tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcggcgaat
     8881 cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta
     8941 gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg
     9001 gcgatcagcc caaaattcca tacgttgaaa gcttcccgac cggcacacca caaagccctt
     9061 acggcaaaag caagctgatg gtggaacaga tcctcaccga tctgcaaaaa gcccagccgg
     9121 actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata
     9181 tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc caggttgctg
     9241 taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg
     9301 gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa
     9361 aactggcgaa caagccaggc gtacacatct acaacctcgg cgctggcgta ggcaacagcg
     9421 tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaat tatcattttg
     9481 caccgcgtcg cgagggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg
     9541 aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc
     9601 agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatcccg
     9661 ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc
     9721 accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg
     9781 cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc
     9841 ccgattacac caggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc
     9901 cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc
     9961 gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat
    10021 tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt
    10081 gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg
    10141 gacagatttg ggcaaatagc ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa
    10201 aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg
    10261 cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg
    10321 ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt
    10381 tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg
    10441 acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg
    10501 gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg
    10561 ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga
    10621 ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg
    10681 gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct
    10741 accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg
    10801 actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg
    10861 cgccacgcga tgaccgtaaa gttcgcgtaa tggcagccga ttatgaaaat cagctcgacg
    10921 agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc
    10981 gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga
    11041 tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg
    11101 tcggaaccgt attgcagcag ctttattatc tgccgctgga cggcgcacaa atcgcgctta
    11161 acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa
    11221 tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgtcgctca ctggggacca
    11281 aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta
    11341 ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct
    11401 tccagcagcc agccctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc
    11461 tggacccaat cgtggcgaaa cgcgtgcggc atatcctgac tgaaaacgcc cgcaccgttg
    11521 aagctgccag cgcgctggag cagggcgacc tgaaacgtat gggcgagttg atggcggagt
    11581 ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag
    11641 aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg
    11701 gcggctgtat cgtcgcgttg atcccggaag agctggtgcc tgccgtacag caagctgtcg
    11761 ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac
    11821 aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg
    11881 actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac
    11941 tttactttca gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg
    12001 tgccagcccg gaatgctatc aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta
    12061 tgccaaccgc atcgccaata gccgttatac ctttgacggt gaaaccgtga ccctttcgcc
    12121 aagtcagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca
    12181 gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca
    12241 gggcttcccg ggtaatctcg gcgcaacggt gcaatatcgt ctgaccgacg ataaccgtat
    12301 ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt
    12361 ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc
    12421 ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc
    12481 cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga
    12541 cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg
    12601 caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac
    12661 caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg
    12721 aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag
    12781 cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc
    12841 cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt
    12901 atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa
    12961 caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg
    13021 tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt aagtatatgg
    13081 ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt
    13141 tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag
    13201 gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac
    13261 gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg
    13321 agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcag
    13381 aaaccgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtca
    13441 ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatcca cgttacgcga
    13501 agctgagcga gaaagaactg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga
    13561 tcccttactg gaatgaaact attctaccgc gtatgaagag cggtgagcgc gtgatcatcg
    13621 ctgcacacgg taactcttta cgtgcgctgg tgaaatatct tgataacatg agtgaagaag
    13681 agattcttga gctgaatatc ccgaccggcg tgccgctggt gtatgagttc gacgagaatt
    13741 tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcagcg aaagcagcgg
    13801 cggttgctaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaatga gaaagccgac
    13861 tgcaagtgag ccggcttttt tgttgctaac aatggagcac attgcctgat gcgacgctgc
    13921 gcgtcttatc aggcctgtgg tgattcatcg gatacgccac tctgacggcg catccgacaa
    13981 ttaaacctta cccgcgacgc gcttttactg cattcgccag ttgacgtaac agagcatcgg
    14041 tatcttccca gccgatgcag gcatcggtga tgctcttacc gtaggccagc ggctccccgc
    14101 tctcgaggct ctgattgcct tccaccagat ggctttccac catcacgcca ataatggctg
    14161 tttcgccacc ggcaatctgc tggcaaacgt cagcacacac atccatctgc tttttgaatt
    14221 gtttggacga gttagcatgg ctgaaatcga tcatcacctg tgctggcagg cctgctttgt
    14281 tcagcccttc tttcacttca gcaacgtgct tcgcgctgta gttaggctct ttaccgccgc
    14341 gcagaatgat atggcaatca ccgttaccgc tggtattcac aatcgccgaa tgcccccatt
    14401 tagttacgga caggaagcag tgcggcgcac cggcggcatt aatggcatcg atagccactt
    14461 taatcgtacc gtctgtgcca tttttaaaac ctaccggaca agaaagacca gacgccagtt
    14521 cgcggtgcac ctgcgattcg gtggtacgtg caccaattgc gccccagctc atcaggtcag
    14581 cgagatattg tggggtgatc atatcgagaa actcacccgc cgctggcaga ccgctgtcgt
    14641 taatatcaag cagcaattta cgggctatac gcagaccgtc gttgatctgg aagctgttat
    14701 ccatatgcgg atcgttaatc agccctttcc agcccaccgt ggtacgcggc ttttcaaaat
    14761 agacgcgcat tacgatttcc agctcatctt tcagctcttc acgcagcgcc agcaagcgag
    14821 tggcatactc ttttgctgcg acaggatcat gaattgagca tgggccaatc accaccaaca
    14881 ggcgatcatc attacctttc aggatcttat ggatcgcttt tcgggcatgg gcaaccgtat
    14941 tcgcggcatt ttcagtagcg gggaattttt ccagcaatgc gacaggagga agtaactctt
    15001 tgatttcttt gatgcgtaaa tcgtcgttct gataattcat gtctgttcca gtgttgccat
    15061 acttatctta gtgaatgcaa tacttccaat ctatatcttc cgtcagaatg tgtaaacggg
    15121 gttttacact atgaacggat taatcctgga attagcaaga aaaacgccag attgtcgcga
    15181 aaaacgagat ctctcctaca atttctaact gtaactcctt tgtttgttaa ttatttcaag
    15241 attctctgct gcgtttcata acctggctga aatcttaaac caatgcctta tattcacctg
    15301 caaatgcact gttggaagag gttatccgac ataacgacca taacaggagc atcctatgaa
    15361 aatgacaaaa ctggccacat tatttctgac tgccactcta agccttgcca gcggtgccgc
    15421 actggctgcc gatagcggag cgcaaactaa taacggccag gcaaacgccg cagctgatgc
    15481 gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca aataacgtcg acaataacgg
    15541 ggtaaatacc ggttctggtg gcacaatgct gcattcggat ggttcttcaa tgaacaatga
    15601 cggaatgacc aaagatgaag agcacaagaa caccatgtgc aaagatggtc gctgtccgga
    15661 tatcaataaa aaggtacaaa ccggtgatgg catcaataat gatgtcgata ccaaaaccga
    15721 cggtaccacg cagtaaccct gcaaaatagc caataatggg agagcggagt ctctcccttt
    15781 taatttttca cttctaaaag cagtatgatg tcataaagtt aagtgagaat aattaaggga
    15841 tgactcgtca tggcgcactc acactcacac acgtcctcac acctgccaga agataataat
    15901 gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt ttatgctggt agaagtcgtt
    15961 ggtggttttc tttctggttc tctggcattg ctggccgatg cgggtcatat gttgaccgat
    16021 actgccgccc tgcttttcgc cctactcgcc gtgcaatttt cacgtcgtcc tcccaccatt
    16081 cgccacactt tcggttggct aagactgacc acgctcgcgg cgtttgtgaa tgccatagcc
    16141 ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag aacgcttccg cacgccgcgt
    16201 ccggtcgagg gcggcatgat gatggcaatt gccgtggctg ggttgctggc aaatatactt
    16261 tctttctggt tacttcatca cggcagcgaa gagaaaaacc tcaacgtacg agcagcggca
    16321 ctgcatgtgc tcggggacct gctgggttcg gttggggcta ttattgccgc gttgattatt
    16381 atctggactg gctggacacc tgctgacccc attctctcga tactggtgtc gcttctggtt
    16441 ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg aattacttga aggtgcaccg
    16501 gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc gggaaatccc ggaagtccgc
    16561 aatgtgcacc atgtacatgt atggatggta ggcgagaagc cggtgatgac gttgcatgtg
    16621 caggtgatcc cgccacacga tcacgatgcc ttgttggatc agatccaaca ctacctgatg
    16681 gatcactatc agattgagca cgccaccatt cagatggaat atcaaccttg tcatgggcca
    16741 gactgccacc tgaatgaagg ggtgtctggc cattcacatc atcaccatta atgggacagc
    16801 gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc ggctgccgtt gagcgcaata
    16861 aaggtcagga tgatgtactc cagagacatg gcgtaaacgc cctgaagtgc aaaaataacg
    16921 acgctaatca cgttaataat cacccacaac agccagtttt ccacatactt acgcgtcatc
    16981 agaatcattg ccacgataga taacaccatc atgcatgaat cccagaacgg gaaagcgtcc
    17041 ggttgcagtt caggcatcac aacctgtaat cctaatgctt gcatgatcat gactgccagg
    17101 cgggtcaaaa atgcaaacac cggattgata aataccgtca tcagaccaat cgaaacaacg
    17161 caaaccgcca accagctgag tgccttcggc aatggcaacc agcgaatttt caactccgcc
    17221 tcgttctgac tggtttgtcg cgaccacgca taccaaccgt aaatattcgc ggcaaagaaa
    17281 aacacctgta atagcaggct ggcatacagc tgaatctgaa agaaaataat gccaaacaag
    17341 gtgacgttga tcaggccaaa gaagtagttg ctgatcttct ccagactggc aaggccaata
    17401 cacagcaacc cggcgatcgt gcctaccgct tcgatccatg agagatcata accgcctgcc
    17461 cctattggta tatgtaccag gatattctgc acactaaaaa aatccatctt tcccccaaag
    17521 cgtaacgtta cgccttatta ttcgttatcc acgtagtgta gccgcaaaat ccagcatacg
    17581 attgagcggc accagcgccc tctctcgcag cctttcatca acatgaacct cgtgattgct
    17641 tccttcctgt tctaatgcct ctgcgatgga ctgaaggcca ttcatggcca tccacggaca
    17701 atgcgcgcag ctgcggcagg ttgcaccctc acctgcggtt ggtgcttcca gtaactcttt
    17761 atctggcact gcctgctgca ttttgtagaa aatgccccga tcggttgcca cgataagccg
    17821 ctgatgtggc aatgttttcg cagcagcgat cagttgactg gtggaaccga ccgcatccgc
    17881 catatcgaca atagcttgtg gtgattctgg atgcaccagt atggcagcat ccgggtattc
    17941 ttcttgcaag cgggttaacg cctgagtctt aaattcatca tgcacaatac aggcaccctg
    18001 ccagcataga atgtctccac ccgtctgttt ttgcacgtaa cgccccagat gtttgtcggg
    18061 tgcccagatg attttttcac ccaaactatc aagatgatca ataagttcga cggcaatgct
    18121 tgaagttacc acccaatctg cgcgcgcttt taccgcagca gaagtgttgg cgtagacgac
    18181 gacagtacga tcgggatggg catcgcaaaa tgcgttaaat tcttcaacag ggcagccgag
    18241 atccagtgaa cattcagcct gaagtgtcgg catcagaatt gttttttccg gactgagaat
    18301 tttggcggtt tctcccataa atctcacccc agcgactaac aaagtagaag cgggatgctt
    18361 tgcaccgaag cgcgccattt ccagagaatc agaaatacag ccaccggttt cttctgccag
    18421 ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc accgcattac gttcttttag
    18481 cagacgtttt atcttctcgc ggtaatacgc tttttcatca atgcttaacg gcgtcggctt
    18541 cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt acgctcatct taccatctcg
    18601 ttttacaggc ttaacgttaa aaccgacatt agcgtaatat tcgctgttat cggtgtaatt
    18661 tgttttatat gctaaacaag atagtcgaat ggtggggaaa agtcactcga attacgccta
    18721 attccaccga cgtagagttg cttgcacagt aagctctgac gatgtcatca ggtttacaaa
    18781 gtgaaattga ggatgcctga atagcaaaaa agcgccttta gggcgctttt ttacattggt
    18841 gggtcgtgca ggatgactcg cttcgctagc ccttcgggcc gtcgccgcga gcggctccgt
    18901 tgtttcgctg cgctcaactc aaacctgctg caggttcgaa tctttaagta tcgcagatag
    18961 caaaaaagcg cctttagggc gcttttttac attggtgggt cgtgcaggat tcgaacctgc
    19021 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccct tgcgggatta
    19081 tgctgcttta atcattcagg atggtgggtc gtgcaggatt cgaacctgcg accaattgat
    19141 taaaagtcaa ctgctctacc aactgagcta acgacccgag tggtgggtga tgacgggatc
    19201 gaaccgccga ccccctcctt gtaagggagg tgctctccca gctgagctaa tcacccgata
    19261 ctgcgctgga tactacaaac tacttccacc gaacccaccg taattagtgg tgggtcgtgc
    19321 aggatgactc gcttcgctcg ccctacgggc cgttgctgac gcaacgttat cctacacgct
    19381 taacatctgt cttcgatgct aaaactggtg ggtcgtgcag gattcgaacc tgcgaccaat
    19441 tgattaaaag tcaactgctc taccaactga gctaacgacc cggtggtggg tgatgacggg
    19501 atcgaaccgc cgaccccctc cttgtaaggg agatgctctc ccagctgagc taatcaccca
    19561 atactacgct ggatactaca actaactatg ttagtggtgg gtcgtgcagg atgactcgac
    19621 ttcgtctcgc ccttcgggcc gttgctgatg caacgttatc cttcacgttt tactaccgct
    19681 tccaccttag cgattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    19741 caactgctct accaactgag ctaacgaccc actttttcgt tgctttcggt ttgtttgata
    19801 tcccgtggca acggcggcat atattactga tttcagactt gagcgcaaca aaaatttcga
    19861 tgcagatcac tcaactgctt atgattcgca cgacacgacc agaaataatg cgacttctgg
    19921 tcgtgtgtta tgcattacat cgcgttcaga cgtttttgtg cctgtttagc gccatcagta
    19981 ccagggtatt tactgataac ctgctggtat acggctttcg cttttgcggt gtcacctttg
    20041 tcctgcatga tgacgccgac tttaaacatc gcatcagcag cctttggtga cttcggatag
    20101 tttttcacta ccgaagcaaa atagtacgcc gcatcatctt ttttaccctt gttgtagttt
    20161 aactgaccca gccaataatt ggcgtttggc aggtaagttg aatcagggta atttttgatg
    20221 aaattctgaa atgccaccat tgcgtcatcc tggcgggatt tatcctgcac cagcgcaata
    20281 gctgcattgt aatccgtgtt tgcatcgccg cttttcaccg gcgcgccagc attcgcagta
    20341 ccagcatcag ctgtcggcgt cgttgatgcc gccgcaccgc tttgatcgct gctggttgat
    20401 tgcgccgctg caccaccgct gctgaggctg tcgatctgca acaggatctg cttttgccgc
    20461 tccacgacct gattcagttg atactgattt tcctgaatct gaccacgcag ggaatcaata
    20521 tcggattgat tatcagaaag ttgttgctgg agttgggtta aaagctggct gtgagcatta
    20581 gaaatacgct caagttgagt gacgcggtct tcgaccgagc ctgagccgac actactgatt
    20641 ggtgcctgag caaaagcggc ccagggggcc gctataccaa ccagtaacga cagactcaat
    20701 agttgatgtc tgaagttact gctcatgcaa ttctcttagt aaaccagtac cgcacgacgg
    20761 tttttggagt atgccgcttc gtcatgaccc agtactgcag gtttttcttt accgtaagaa
    20821 acgatggaga tctggtctgc agaaacgcct ttaccctgca ggtacatctt aacggcgttc
    20881 gcacgacgtt cacccaggga gatgttgtat tccggagtac cacgttcgtc cgcgtgacct
    20941 tctacggtga ctttgtaaga cgggttgcta cgcaggaagt ttgcatgtgc atccagcatt
    21001 tgagcgaagt cagaacggat atcgtacttg tccagatcga agtaaacgat gttgttctgc
    21061 tgcagctgtt gcatttgcag acgagcctgc tcttcggaag acatgttgcc gttgccgccg
    21121 ttcgcatcca taccagtgcc ggcacccagc atgccttcgc tgccgtcatt gctggcgttc
    21181 ttgttggaag aacatgccgc aattgccata acaggcagag caatcatcag ccctttcagc
    21241 actttgttca gttgcatttc aatgattcct ttactattca attaattatt atcacagata
    21301 cggcgaccag gcagggaatt tgacctgtcc atcagttgcc ggaagacgcg ctttgaaacg
    21361 cccatctgta gaaaccaaat tcagcacgga tcccatcccc tgagaagagc tgtagattac
    21421 catagtgccg ttaggtgcca gacttggcgt ttcatccagg aacgtggacg acagaacttg
    21481 tacgcctccc gttgccagat cttgtttggc aatgtgctgc tgcccaccat tggagctgac
    21541 cattaccata aatttaccgt cgctgctgac atccgcatcc tggttctgcg aaccttccca
    21601 ggtaatacgt tgtggcgcac cgccgttgat attcacttta taaacctgcg gacgaccggc
    21661 ctggtcagaa gtaaatgcca ggttctggct atccgggaac caggtcggtt cggtattgtt
    21721 actgcgacca tcagtcacct ggcggatctg accagaagcc aaatccatta cgtacaggtt
    21781 cagactaccg gttttcgaca aggcgaatgc cagtttgctg ccgtctggcg agaatgcagg
    21841 tgcaccgttg tgacgcggga atgaagccac ctgacgtaca gcgccatttg ccagcgtctg
    21901 aataaccagc gcggaacgac cgctttcgaa ggtcacataa gccagtttag aaccgtctgg
    21961 tgaccacgcc ggagacatca gcggctgcgg tgaacggtga acgacaaact ggttgtaacc
    22021 gtcatagtca gatacgcgca gttcatacgg gaactgaccg ccgttggtct gaacaacgta
    22081 ggcaatacga gtacggaacg cacctttaat gccggtcagt ttttcaaaca cttcatcact
    22141 ggcggtatga ccagcataac gcagccactg cttgttcact ttgtacgagt tctgagcaag
    22201 tacagtaccc ggtgcgccgc cagtgtcaac aagttgataa gcaacattgt aagaaccatc
    22261 cggattcgga gtgacctgac cgacaactac agcgtcaatg cccagtgcgg accatgcagc
    22321 tggttgtact tcctgcgcac tacccggctg ctgtggcaga cgagcgcgat ctaacggatt
    22381 aaatttaccg ctgttacgca agtcagcagc aacgatgccg ccaatatctt caggtgccgc
    22441 accaggcccc gcccactgga aaggaacaac accaatagga cgaccggaat ctacaccgct
    22501 gtcgatcaca atgcggactt cagcatgcag aactgatgcc cacagtatga gaaaaccaaa
    22561 tgctactcgt aatgcctgct tcatcatatc tcccttatct ggaccgatgg cccacgataa
    22621 tttagcagaa tgttaacaaa ctaaaataca caaaactacc agaaccccgt ggcaaccggt
    22681 gcctgatgtt gaccgtccga acagtcaaca tcgcgattac ggtttgaagt ccaatggcgc
    22741 gtttttgaac acttcatata ctgcctggct tggtggtttc gggatcttcg caagtttagc
    22801 tgctgccaac gcagcctgac aaagtgcggg atcgccacct tcaggtttga tatccagtaa
    22861 cataccatcg ggtgccagtt ttatgcgcag cgtacaggtt ttgcctgcat aggacgatgc
    22921 gtcatagaac ttactttcga tagcagattt aatctgcccg gcatagttat tgatatcggc
    22981 ccctgatgcg ccattgtttt tagtattacc actcccggca ggcgaagcat tgttcccttt
    23041 cgcccctccc cccgttttcg gtgcattctt accagagctt agctcaccga aaatatcatc
    23101 tgcctctgcg gccgcttttg ctgcagcggc tttttcagct gcagcttttg ccgctgccgc
    23161 ttttttgtct gcggcggctt tttctgccgc tgctttctta tcagctgctg ccttttcagc
    23221 agccgctttt tctgctgctt ttttgtcggc tgcagctttc tctgctgccg ctttcttatc
    23281 agctgcagcc ttttcagcag ccgctttctt ctcagcttct gctttggctt tttcagcagc
    23341 ttcagttgcc gctttctttc ttgcttcagc tgcagctgct tctgccgctt ccgctttctt
    23401 cttcagtgcc gcagcggctg cctcggcttt tttctgcgct tcggctgcgg ctttggcggc
    23461 ttctgcttct gcttttttct ttgcgtctgc agccgctttc ttcgctgctt cttccgcagc
    23521 tttagcatct gcttcggcct tcgctttagc atctgccgcc gctttcgctg ccgcctcttc
    23581 agcttgcttc tgctttaact cggcctgttt tgcggcttct tcagcctgct ttttctgctc
    23641 ctgagccgct aaccgctctt tctcaagttg cttcaggcgt tcctgttcag ccgcttgttt
    23701 ctcacgcagt tcttcagcag cctgctgttc cttcatcttg cgctgttcat cagaacgctt
    23761 cgcgcttgat tcctggcttt gcatgcgttt gtactgctca actaccgcac ctgaatcaac
    23821 catgacagcg tcgatggacg aaccaccgcc gcctccggct gaagcttcta tattctcatc
    23881 gaacgaactc cagatcagcg ccgcaaataa gatgacatgc agcactgctg aaataattat
    23941 cgcccgcttg agcttgtcgt tttgttcggt tgcctttgac actctcggtt tccaaaaact
    24001 gttcgcctgt tacccgctct ctttcaagca agggaaacgc agatgtttag ataggctgcg
    24061 tcattaaacc aaccgatttc acacccgcac tatgtaacaa gttcagtgct ttaattattt
    24121 catcgtaagg cacatctttt gcgccaccga tcagaaagac cgttttcggg ttggccttga
    24181 aacggctgga cacttccgcc accacctgct ctggtggtaa acgctccagg cgatctttct
    24241 caaccaccac ggtgtactga ccaataccag acacttcaac aatcactggc ggattatcgt
    24301 tactgctcac cgcctgtgat tcagtagcgt ctggcagatc gacctccacg ctctgggtga
    24361 tgatgggcgc tgtcgccatg aagatcagca acagcaccag cagtacgtcc agcaacggta
    24421 caatgttgat ttcggactta agttcgcgac gacctcgtcc acgcgctctg gccatgactt
    24481 accccttatt gctctcgcta acggtaaacg cctggcggtg cagaatcgcg gtaaactctt
    24541 ccataaagtt gtcgtaattc agttccagtt tgtttacgcg ctggttgagg cggttgtagg
    24601 ccataactgc cgggatagcg gcaaacagac caattgcagt cgcaatcaac gcttctgcga
    24661 taccgggcgc aaccatttgc agtgttgctt gttttaccgc cccgagggcg ataaaggcgt
    24721 gcatgatccc ccagaccgta ccaaacagac caatatacgg gctgatggag ccaaccgtac
    24781 cgaggaacgg aatgtgcgtt tccagatttt caagttcacg gttcatggag atacgcatag
    24841 cacgcgacgc cccttccact acggcttccg gcgcatggct attggcacga tgcaggcgca
    24901 caaactcttt gaacccgctg taaaatattt gttccgaacc agtcagatta tcccgtttcc
    24961 cctgactctc ttgatagagg cgagagagtt cgattccaga ccagaattta tcttcaaacg
    25021 cttcggcttc gcgcgccgct gcgttaagaa tacgggtccg ctggataata atggcccagg
    25081 atgcgattga aaaaccaatc aaaatcaaca tgataagttt aaccagaagg ctagccttca
    25141 ggaacaaatc aaggatattc atgtcagtca ctgcttaaac tccgcgacaa tagacttggg
    25201 aagcgcacga ggcttcattt tgagtgggtc aacgcaaaca accagaacct ctgcttcatt
    25261 cagcaaagta ttctcggcgt tgacaatacg ttgcgtgaaa accaaagagg tgccacgcat
    25321 tgatgttatt tcagtctgta tttcgagcat atcgtcgagc cgcgcaggtg cgtaatattc
    25381 caccgtcatt ttacgtacca caaaggcaac gcgttcagcc atcagcgcct gctgactgaa
    25441 gtgatgatga cgcagcatct ctgtgcgtgc tctttcataa aaagcgacgt aactggcgtg
    25501 gtacaccaca ccaccggcat cggtatcttc atagtagacg cgaaccggcc atcgaaacag
    25561 cgttgtattc actttacatc ccggtaatgc aacaaaagtt agagctttta aacgctgcta
    25621 ctatacgcgc gcgattgagg tttgggaatg agggcaagtt aagggagcgt aaaaatttat
    25681 gggcctggag cggcccataa ttttttaaag agaattctga tttaaaagaa gaagaaaatc
    25741 agcccgacaa tgagaacaat atcggcaagc aatgggcaaa aaatcccttg ccaaagaacc
    25801 ttctgcggac gaaagcccac gccgtgaatc acaccagcac agacggccca catcagcaat
    25861 aaaccatgcc agatttccag ttcactggtc ttcgcggcaa aacgagatgg gtcccaaaac
    25921 atacatcctg ctaacagaag cgccatcacg aaggaaagcg cccgcagggg gcgcttgtcc
    25981 attaccgcat acaaagtcgc gataatctta ctcatcagat gtcttcttga ccggctttgc
    26041 ctgattcgac gtgttcaagc gccagcgcgg tgattacccc aaacgaacag gcaagaagcg
    26101 ttcccagaat ccatgcgaaa taccacattt ttagctcctt acttagtaca gagagtgggt
    26161 gttacgttca atatcttctt tggtgatacg accgaacatt ttccagtaac accaggcggt
    26221 gtagagcaga atgatcggta ccagaaccac cgcaacccag gtcatgacgt taagcgtcag
    26281 ctggctggaa gttgcatccc acattgtcag acttgcgttc atcatggtgc tggacggcat
    26341 cacaaacggg aacattgcga taccggctgt caggatgatg caggccagcg tcagggagga
    26401 gaacacaaac gcccacgcgg ctttatccat acgtgcagtc aggatggtca gcagcggcag
    26461 aaccacaccc agtgccggaa tagcccacag aattggcgtg ttgttgaagt taaccagcca
    26521 tgcgccagct tcacgaacca cttctttatt cagtgggtta gaggctgcgt aatggtccat
    26581 tgtcgatttc acgacataac catcgatacc gtacatcacc catacgccag ccagtgcgaa
    26641 acagaccagt gtcaccagcg cagccacctg agccgttgca cgggtacgca ggtgcagttc
    26701 gcccacggta cgcatttgca gataggttgc gccctgagta atgatcatcc ctacgctcac
    26761 cacgcctgcc agcaggccga acgggttaag caactggaag aagttaccgg tgtagtacag
    26821 acgcagatat tcatcaacgt tgaacggtac gccctgcaac aggttaccga acgctacacc
    26881 aattaccagc ggcggaacga agctaccaat gaagatgccc cagtcccaca tgttacgcca
    26941 gcgggtttct tcaatcttgg agcggtagtc aaaaccgacc ggacggaaga acaaagacgc
    27001 cagcacgagg atcatcgcca catagaagcc ggagaacgca gcggcataga ccatcggcca
    27061 ggcagcaaag agtgcgccgc ccgcggtgat cagccaaacc tggttaccgt cccagtgtgg
    27121 tgcaatggag ttaatcataa ttcgacgctc ggtgtcgtta cgaccgagga aacgggtgag
    27181 catgcccacc cccatgtcga aaccgtcagt gactgcaaaa ccaatcagca gaacgccaac
    27241 cagcagccac cagataaaac gcaatacttc ataatcgatc atttgacgac tcctgtctta
    27301 gcgtgccggc tgagtagtcg tggaagactg ctcaaagtga tagcgaccgg ttttcaggct
    27361 gcttgggccg aggcgtgcaa atttgaacat taagaacaat tctgccacca ggaacagggt
    27421 atacaggccg caaatcagca ccattgagaa gatgagatcg cctgcggtca gtgacgagtt
    27481 cgccacagct gtcggcagca cttcaccgat agcccacggt tggcggccat attcagccac
    27541 gaaccagccc gcttctacag caatccacgg cagcggaata ccgtacagcg cggcgcgcag
    27601 aagccatttt ttctcgccaa tgcggttgcg gatgacactc cagaaagaga gcgcgatgat
    27661 tgccagaagc aggaagccac acgccaccat gatacggaac gcaaagtaca gcggcgctac
    27721 acgcgggatg gagtctttgg ttgcctgttg aatctgcgct tcagtcgcat cagccacgtt
    27781 tggcgtatag cgtttcagca gcagaccgta accgaggtct ttcttcatgc tattgaactg
    27841 gtcacgaacc gcctggtcgg tagaaccaga acgcagttgt tcgagcagag agtacgcctt
    27901 catcccgtta cgaatgcgtt cttcatgctg caccatcagc tctttcaggc cgataaccgg
    27961 ggtatccacg gaacgcgttg caatgatgcc cagtgcgtaa gggatctgaa tcgcaaattt
    28021 gttcgtctcc tcttcctgat caggaatgcc gaacagagta aaggcagcag gcgcaggttg
    28081 cgtttcccac tcggcttcaa tagcagccag tttggttttc tgcacgtcgc ccatttcgta
    28141 gccggattca tcacccagaa caataacaga cagaacagca gccataccga agctggcagc
    28201 gatagcaaag gagcgtttag cgaaggcgaa gtcacgacct ttcagcatat accatgcgct
    28261 gataccgagg atgaacatcg cgccagtcac ataaccagac gctacagtgt gaacgaattt
    28321 cacctgagca accgggttaa gcaccagctc ggagaagctc accatctcca tacgcatagt
    28381 ttcaaagttg aaatcggacg cgattgggtt ttgcatccag ccgttcgcaa ccagaatcca
    28441 cagtgcggac aggtttgaac cgagcgccac cagccaggtg acacacatat gctgaacttt
    28501 acccagacga tcccaaccga agaagaacag acctacaaag gtggattcga ggaagaaggc
    28561 catcagacct tcgattgcca gcggcgcacc gaagatatcc cctacatagt gggaatagta
    28621 agaccagtta gtcccgaact ggaactccat ggtcagaccg gtagccacac ccagagcgaa
    28681 gttgatacca aacaacttgc cccagaactt ggtcatatct ttataaatct gtttgccgga
    28741 gaggacgtag accgtttcca taatggccag caggaacgcc ataccgagcg tcagtggcac
    28801 aaaaaggaag tggtacatcg cggtcaaggc aaactgtaag cgcgacagtt cgactatatc
    28861 taacatcatg actccttgct catcgcatga agactccgag agtgaacccc gttagaaagg
    28921 gtcacacgca tgccccaata gaaatttgtt tgaacacttt catcatacca tgtgaatgca
    28981 cacaaaaacg gcgtaaagaa aggttgcaaa catgttaata aaaactcaaa ttgatcccac
    29041 gtatatatta cgccgcaaaa tccttacaat aaacaggttt ttgttgaagc aaatttgcat
    29101 ttttcgacgg tgatcaattt atagctaaat taccgccttt cagccaattt gatcgagaac
    29161 aatttatctc tttttgatgc ccatttccaa gacttataca ttgataaata tcaattccta
    29221 caaattatgt
//
LOCUS       DAZFWL020000049        28944 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFWL020000049 DAZFWL020000000
VERSION     DAZFWL020000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.049"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            279..1145
                     /gene="folD"
                     /locus_tag="ACHKNP_RS11590"
                     /old_locus_tag="pgaptmp_002318"
     CDS             279..1145
                     /gene="folD"
                     /locus_tag="ACHKNP_RS11590"
                     /old_locus_tag="pgaptmp_002318"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HGU4704474.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1147..1359
                     /gene="ybcJ"
                     /locus_tag="ACHKNP_RS11595"
                     /old_locus_tag="pgaptmp_002319"
     CDS             1147..1359
                     /gene="ybcJ"
                     /locus_tag="ACHKNP_RS11595"
                     /old_locus_tag="pgaptmp_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HGU4704475.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVEVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1467..1988
                     /gene="ybcI"
                     /locus_tag="ACHKNP_RS11600"
                     /old_locus_tag="pgaptmp_002320"
     CDS             1467..1988
                     /gene="ybcI"
                     /locus_tag="ACHKNP_RS11600"
                     /old_locus_tag="pgaptmp_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4704476.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHREFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(2024..3409)
                     /gene="cysS"
                     /locus_tag="ACHKNP_RS11605"
                     /old_locus_tag="pgaptmp_002321"
     CDS             complement(2024..3409)
                     /gene="cysS"
                     /locus_tag="ACHKNP_RS11605"
                     /old_locus_tag="pgaptmp_002321"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGU4704477.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYIALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3583..4077
                     /gene="ppiB"
                     /locus_tag="ACHKNP_RS11610"
                     /old_locus_tag="pgaptmp_002322"
     CDS             3583..4077
                     /gene="ppiB"
                     /locus_tag="ACHKNP_RS11610"
                     /old_locus_tag="pgaptmp_002322"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HGU4704478.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4080..4802
                     /gene="lpxH"
                     /locus_tag="ACHKNP_RS11615"
                     /old_locus_tag="pgaptmp_002323"
     CDS             4080..4802
                     /gene="lpxH"
                     /locus_tag="ACHKNP_RS11615"
                     /old_locus_tag="pgaptmp_002323"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HGU4704479.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4920..5429
                     /gene="purE"
                     /locus_tag="ACHKNP_RS11620"
                     /old_locus_tag="pgaptmp_002324"
     CDS             4920..5429
                     /gene="purE"
                     /locus_tag="ACHKNP_RS11620"
                     /old_locus_tag="pgaptmp_002324"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGU4704480.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5426..6493
                     /gene="purK"
                     /locus_tag="ACHKNP_RS11625"
                     /old_locus_tag="pgaptmp_002325"
     CDS             5426..6493
                     /gene="purK"
                     /locus_tag="ACHKNP_RS11625"
                     /old_locus_tag="pgaptmp_002325"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGU4704481.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6630..7523)
                     /gene="ybcF"
                     /locus_tag="ACHKNP_RS11630"
                     /old_locus_tag="pgaptmp_002326"
     CDS             complement(6630..7523)
                     /gene="ybcF"
                     /locus_tag="ACHKNP_RS11630"
                     /old_locus_tag="pgaptmp_002326"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU4704482.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFTKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7520..8335)
                     /gene="ylbF"
                     /locus_tag="ACHKNP_RS11635"
                     /old_locus_tag="pgaptmp_002327"
     CDS             complement(7520..8335)
                     /gene="ylbF"
                     /locus_tag="ACHKNP_RS11635"
                     /old_locus_tag="pgaptmp_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU4704483.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8346..9605)
                     /gene="ylbE"
                     /locus_tag="ACHKNP_RS11640"
                     /old_locus_tag="pgaptmp_002328"
     CDS             complement(8346..9605)
                     /gene="ylbE"
                     /locus_tag="ACHKNP_RS11640"
                     /old_locus_tag="pgaptmp_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU4704484.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9615..11282)
                     /gene="fdrA"
                     /locus_tag="ACHKNP_RS11645"
                     /old_locus_tag="pgaptmp_002329"
     CDS             complement(9615..11282)
                     /gene="fdrA"
                     /locus_tag="ACHKNP_RS11645"
                     /old_locus_tag="pgaptmp_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU4704485.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELTNQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11597..12646
                     /gene="allD"
                     /locus_tag="ACHKNP_RS11650"
                     /old_locus_tag="pgaptmp_002330"
     CDS             11597..12646
                     /gene="allD"
                     /locus_tag="ACHKNP_RS11650"
                     /old_locus_tag="pgaptmp_002330"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HGU4704486.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12668..13903
                     /gene="allC"
                     /locus_tag="ACHKNP_RS11655"
                     /old_locus_tag="pgaptmp_002331"
     CDS             12668..13903
                     /gene="allC"
                     /locus_tag="ACHKNP_RS11655"
                     /old_locus_tag="pgaptmp_002331"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HGU4704487.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13914..14699
                     /gene="allE"
                     /locus_tag="ACHKNP_RS11660"
                     /old_locus_tag="pgaptmp_002332"
     CDS             13914..14699
                     /gene="allE"
                     /locus_tag="ACHKNP_RS11660"
                     /old_locus_tag="pgaptmp_002332"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HGU4704488.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14828..15973)
                     /gene="glxK"
                     /locus_tag="ACHKNP_RS11665"
                     /old_locus_tag="pgaptmp_002333"
     CDS             complement(14828..15973)
                     /gene="glxK"
                     /locus_tag="ACHKNP_RS11665"
                     /old_locus_tag="pgaptmp_002333"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HGU4704489.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(15995..17295)
                     /gene="ybbY"
                     /locus_tag="ACHKNP_RS11670"
                     /old_locus_tag="pgaptmp_002334"
                     /pseudo
     CDS             complement(15995..17295)
                     /gene="ybbY"
                     /locus_tag="ACHKNP_RS11670"
                     /old_locus_tag="pgaptmp_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
     gene            complement(17352..18713)
                     /gene="allB"
                     /locus_tag="ACHKNP_RS11675"
                     /old_locus_tag="pgaptmp_002335"
     CDS             complement(17352..18713)
                     /gene="allB"
                     /locus_tag="ACHKNP_RS11675"
                     /old_locus_tag="pgaptmp_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HGU4704490.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18773..20227)
                     /gene="ybbW"
                     /locus_tag="ACHKNP_RS11680"
                     /old_locus_tag="pgaptmp_002336"
     CDS             complement(18773..20227)
                     /gene="ybbW"
                     /locus_tag="ACHKNP_RS11680"
                     /old_locus_tag="pgaptmp_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HGU4704491.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIHFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(20396..21274)
                     /gene="glxR"
                     /locus_tag="ACHKNP_RS11685"
                     /old_locus_tag="pgaptmp_002337"
     CDS             complement(20396..21274)
                     /gene="glxR"
                     /locus_tag="ACHKNP_RS11685"
                     /old_locus_tag="pgaptmp_002337"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU4704492.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21374..22150)
                     /gene="hyi"
                     /locus_tag="ACHKNP_RS11690"
                     /old_locus_tag="pgaptmp_002338"
     CDS             complement(21374..22150)
                     /gene="hyi"
                     /locus_tag="ACHKNP_RS11690"
                     /old_locus_tag="pgaptmp_002338"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HGU4704493.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYTPQTTTEAGLRWMDPYR"
     gene            complement(22163..23944)
                     /gene="gcl"
                     /locus_tag="ACHKNP_RS11695"
                     /old_locus_tag="pgaptmp_002339"
     CDS             complement(22163..23944)
                     /gene="gcl"
                     /locus_tag="ACHKNP_RS11695"
                     /old_locus_tag="pgaptmp_002339"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HGU4704494.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(24034..24849)
                     /gene="allR"
                     /locus_tag="ACHKNP_RS11700"
                     /old_locus_tag="pgaptmp_002340"
     CDS             complement(24034..24849)
                     /gene="allR"
                     /locus_tag="ACHKNP_RS11700"
                     /old_locus_tag="pgaptmp_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HGU4704495.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(24927..25409)
                     /gene="allA"
                     /locus_tag="ACHKNP_RS11705"
                     /old_locus_tag="pgaptmp_002341"
     CDS             complement(24927..25409)
                     /gene="allA"
                     /locus_tag="ACHKNP_RS11705"
                     /old_locus_tag="pgaptmp_002341"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HGU4704496.1"
                     /translation="MKLQVLLLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25639..26565
                     /gene="allS"
                     /locus_tag="ACHKNP_RS11710"
                     /old_locus_tag="pgaptmp_002342"
     CDS             25639..26565
                     /gene="allS"
                     /locus_tag="ACHKNP_RS11710"
                     /old_locus_tag="pgaptmp_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HGU4704497.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26634..27728
                     /gene="mnmH"
                     /locus_tag="ACHKNP_RS11715"
                     /old_locus_tag="pgaptmp_002343"
     CDS             26634..27728
                     /gene="mnmH"
                     /locus_tag="ACHKNP_RS11715"
                     /old_locus_tag="pgaptmp_002343"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HGU4704498.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            27844..28209
                     /gene="ylbG"
                     /locus_tag="ACHKNP_RS11720"
                     /old_locus_tag="pgaptmp_002344"
                     /pseudo
     CDS             27844..28209
                     /gene="ylbG"
                     /locus_tag="ACHKNP_RS11720"
                     /old_locus_tag="pgaptmp_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(28730..>28944)
                     /locus_tag="ACHKNP_RS11725"
                     /old_locus_tag="pgaptmp_002345"
     CDS             complement(28730..>28944)
                     /locus_tag="ACHKNP_RS11725"
                     /old_locus_tag="pgaptmp_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704499.1"
                     /translation="RFKKTLTVRAREQVAKIVSKNDPDTKKVWCKYGKIPGQGDGVNL
                     FFVGEINVTHYFITNIGAGLPDACAE"
BASE COUNT         7137 a         7828 c         7191 g         6788 t
ORIGIN      
        1 cggtaaatta ccaggctgaa aattcttttt atattgtcag gtatttctta aattatctta
       61 atccttaaac aaggaaataa atcagttcca gatttacaac gccatcatgg acgaaaaatg
      121 aagctttcaa tctcagcgac ctcagcgacg gtgcgcctca ccttcgcaag aggtcgcttc
      181 acgcgataaa tctgaaacga aacctgacag cgcgccccgc ttctgacaaa ataggcgcat
      241 ccccttcgac ctacgtaaca gatggaatcc tctctctgat ggcagcaaag attattgacg
      301 gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca aaaagttcag gcgcgtattg
      361 cagccggact gcgggcacca ggactggccg ttgtgctggt gggtagtaac cctgcatcgc
      421 aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt cgggttcgtc tcccgctctt
      481 atgacctccc ggaaaccacc agcgaagcgg agctgttgga gcttatcgat gcgctgaatg
      541 ccgacaacac catcgatggc attctggttc aactgccgtt accggcgggt attgataacg
      601 tcaaagtgct ggaacgtatt catccggaca aagacgtgga cggtttccat ccttataacg
      661 tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg caccccgcgc ggtatcgtca
      721 cgctgcttga gcgttacaac attgatacct tcggcctcaa cgccgtggtg attggcgcat
      781 cgaatatcgt cggtcgtcca atgagcatgg aactgttgct cgcaggttgc accactaccg
      841 tgactcaccg tttcactaaa aatctgcgtc accacgtaga aaatgccgat ctgttgatcg
      901 ttgccgtcgg caagccgggc tttattcctg gtgactggat caaagaaggc gcaattgtga
      961 ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt gggcgacgtc gtgtttgaag
     1021 acgcggctaa acgcgcctca tacattacgc ctgttccggg cggcgttggc ccgatgacgg
     1081 ttgccacgct gattgaaaac acgctacagg cgtgcgttga atatcatgat ccacaggatg
     1141 agtaacatgg cgacattttc tttaggtaaa catccgcacg ttgagctgtg cgacttgctg
     1201 aaactggaag gctggagcga aagcggcgcg caggcgaaaa tcgcgattgc cgaaggccag
     1261 gtggaagtcg acggtgcggt tgaaacgcgc aaacgctgca aaatcgtcgc cggtcagaca
     1321 gtgagttttg caggtcacag cgtacaggtt gttgcctgat ccctttcccg gcctgtatca
     1381 acaggccggt tctcttttca gacttttgcc ctctacgccc cgttcatcca tgctgatagc
     1441 atcgacattt tgccgaagcg accatcatgc caaccgttat tacgcacgcc gccgttcccc
     1501 tatgcattgg tttagggttg ggttcgaaag tcatccctcc gcgtctgtta tttgccggaa
     1561 tcatcctggc gatgctgccc gacgccgacg tattgtcgtt taaatttggc gttgcttacg
     1621 gcaatgtttt tggtcatcgc gagtttaccc attcgctggt gtttgcgttt gttgtcccgc
     1681 tgttatgtgt gctgattgga cgccgatggt tcagggctgg gctgattcgc tgctggctgt
     1741 ttttaaccgt ctcgttgcta tcgcacagct tgctggattc ggtaaccact ggcggtaagg
     1801 gcgttggctg gctgtggccg tggtcagatg aacgcttttt cgctccctgg caggtgatta
     1861 aagtcgcgcc gtttgcattg tctcgttaca ccacgccgta cgggcatcag gtgattattt
     1921 ctgaattgat gtgggtgtgg ttgccgggga tggtgcttat gggaatgttg tggtggcgca
     1981 ggcgataacc ggatgcgaaa actcgcatcc ggcaacagcg caattactta cgacgccagg
     2041 tggtcccttg cgggccatct tccagcacga tccccatctc gttaagacgg tcacgcgccg
     2101 catctgccgc cgcccagtct ttcgctttac gggcatccag acgctgttga attaacgctt
     2161 caatctcagc cacttcgctg tcgtctgcct gcgcaccgct ttgcaggaac gcttccggtt
     2221 cttgctccag caggcccaat acagcggaaa gtttacgcag gtgagatgcc attgcattcg
     2281 ccgctgccat atcttctgct ttcagacggt tcacttcacg cgccatatca aacagcacgg
     2341 aataggcttc cggggtgttg aaatcgtcgt ccatcgcttc aataaagcgc gcttcaaacg
     2401 cttcgccacc ggcaggcgca acggttttat ctgtgccgcg cagcgcaatg tagaggcgct
     2461 ccagcgccgc acgcgcctgc ttcaggttct cttcgctgta gttcagttgg ctgcgatagt
     2521 ggcccgacat caggaagtaa cgcacggttt ccgcgtcgta gtatttcagc acatcgcgca
     2581 cggtaaagaa gttacccagc gatttggaca tcttctcgcg gtcaaccatc accatccccg
     2641 agtgcatcca gtagttcaca tactggccat catgggcaca ggtggactgc gcgatttcgt
     2701 tctcgtggtg cgggaacatc aggtctgaac cgccgccgtg gatatcaaag tggttaccca
     2761 gctgcttgca gttcattgcc gaacattcaa tgtgccagcc tggacggccc gcgccccacg
     2821 gagacggcca gctcggttcg ccctctttcg acatcttcca cagaacgaag tccatcgggt
     2881 tgcgtttgtc gtcgaccacg tcaacgcgcg cacctgcctg cagctggtcg agatcctgac
     2941 gcgacagcac gccatatgtc ggatcggtcg ggacgtcgaa catcacgtcg ccgttgtccg
     3001 ccacataagc gtgacctttg gcgatcagtt gttcagtgag ttcaataatt tctgcgatat
     3061 ggtgcgtcgc gcgcggctcc atatccgggc gcagaatgtt caaagcatca aaatctttgt
     3121 gcatttcggc gatcatgcga tccaccagcg ccacaaagct ttcgccattt tcattggcgc
     3181 gtttgatgat tttgtcgtcg atatcggtaa tgttgcgcac atacttcagt ttatagccga
     3241 ggaaacgcag atagcgcgca accacgtcaa aagcaacaaa ggtacgcccg tgaccgatat
     3301 gacagagatc gtaaacggtg attccacaca catacatgcc gacttccccg gcgtgaatag
     3361 gcttaaattc ctctttttgg cgtgtcagag tattgaagat ttttagcatc gaagattccg
     3421 tttagacatg tgtgggtaat tgagttgcgt ataataccca tatttcccgc gcgaatcagc
     3481 atacattgcg tgatgatccg atcgtgcggt tatgctataa caccacccta tatatgaccc
     3541 gaactgggtt gaagcaccaa tcaaacggaa caggatgcaa aaatggttac tttccacacc
     3601 aatcacggcg atattgtcat caaaactttt gacgataaag cacctgaaac agttaaaaac
     3661 ttcctggact actgccgcga aggtttttac aacaacacca ttttccaccg tgttatcaac
     3721 ggctttatga ttcagggcgg cggttttgaa ccgggcatga aacaaaaagc caccaaagaa
     3781 ccgatcaaaa acgaagccaa caacggtctg aaaaataccc gtggtacgct ggcaatggcc
     3841 cgtactcagg ctccgcactc tgcaactgca cagttcttca tcaacgtggt tgataacgac
     3901 ttcctgaact tctctggcga aagcctgcaa ggttggggct actgcgtgtt tgctgaagtg
     3961 gttgaaggca tggacgtggt agacaaaatc aaaggtgttg caaccggtcg tagcggtatg
     4021 caccaggacg tgccaaaaga agacgttatc attgaaagcg tgaccgttag cgagtaatcg
     4081 tggcgacact ctttattgca gatcttcatc tctgcgtgga agaaccggcg atcaccgccg
     4141 gttttctgcg ttttttagcg ggggaagccc gcaaggccga cgcgctgtat attcttggcg
     4201 atctgtttga agcatggatt ggcgacgacg atcccaaccc actccatcgc cagatggcgg
     4261 cagcgatcaa agcggtgtcc gattccggcg ttccctgtta tttcattcat ggcaaccgtg
     4321 attttctgct cggcaaacgc tttgcccgtg aaagcggcat gacattattg ccggaagaaa
     4381 aggtgctcga actttatggt cgccgggtgt tgattatgca tggcgacacg ctgtgcaccg
     4441 atgacgcggg ttatcaggct tttcgcgcca aagtccacaa accctggctg caaatgctat
     4501 tcctcgccct gccgttgttt gtgcgcaaac gcattgccgc gcgaatgcga gcgaacagta
     4561 aagaagccaa cagcagtaaa tcgctggcga tcatggacgt taaccaaaac gcggtagtca
     4621 gtgcgatgga aaaacatcag gtgcaatggc tgatccacgg acatacccat cgcccggcgg
     4681 tacatgaact tatcgccaac cagcaacctg cttttcgcgt ggtactcggt gcctggcata
     4741 cggaaggttc gatggtgaaa gtcacggcgg atgacgttga gctgattcat tttccgtttt
     4801 aaaaaacccg caactttgct gatttcacag ccacgcaacc gttttccttg ctctctttcc
     4861 gtgctattct ctgtgccctc taaagccgag agttgtgcac cacaggagtt ttaagacgca
     4921 tgtcttcccg caataatccg gcgcgtgtcg ccatcgtgat ggggtccaaa agcgactggg
     4981 ctaccatgca gttcgccgcc gaaatcttcg aaatcctgaa tgtcccgcac cacgttgaag
     5041 tggtttctgc tcaccgcacc cccgataagc tgttcagctt cgccgaaagc gccgaagaga
     5101 acggttatca ggtgattatt gcgggcgcag gcggcgcagc gcacctgcct ggtatgattg
     5161 ccgccaaaac gctggtgcca gtgctgggcg tgccagtaca gagcgccgca ctgagcggtg
     5221 tcgatagcct ctactccatc gtacaaatgc cgcgcggcat tccggtgggt acgctggcga
     5281 ttggtaaagc tggcgcggca aacgcggcgt tactggcagc acaaattctt gcgactcatg
     5341 ataaagaact gcaccagcgt ctgaatgact ggcgcaaagc ccagaccgac gaagtgctgg
     5401 aaaacccgga cccgcgaggt gcggcatgaa acaggtttgc gtcctcggta acgggcagtt
     5461 aggccgtatg ctgcgtcagg caggtgaacc gttaggcatt gctgtctggc cggtcgggct
     5521 ggacgctgaa ccggcggcgg tgccttttca acaaagcgtg attaccgctg agatcgaacg
     5581 ctggccggaa accgcattaa cccgcgagct ggcgcgtcat ccggcctttg tgaaccgcga
     5641 tgtgttcccg attattgccg accgtctgac tcagaagcag cttttcgata agctccacct
     5701 gccgaccgca ccgtggcagt tacttgccga tcgcagcgag tggcctgcgg tgtttgagcg
     5761 tttaggtgaa ctggcgattg ttaagcgtcg cactggtggc tatgacggtc gcggtcaatg
     5821 gcgtttacgt gccaatgaaa ccgaacagtt accggcagag tgttacggcg aatgtattgt
     5881 cgagcagggc attaacttct ctggtgaagt gtcgctggtt ggcgcacgcg gatttgatgg
     5941 cagcaccgtg ttttatccgc tgacgcataa cctgcatcag gacggtattt tgcgcaccag
     6001 cgtcgctttt ccgcaggcca atgcgcagca gcaagcgcaa gccgaagaga tgctgtcggc
     6061 gattatgcag gagctgggct atgtgggcgt gatggcgatg gagtgttttg tcaccccgca
     6121 aggtctgctg atcaacgaac tggcaccgcg tgtgcataac agcggtcact ggacacaaaa
     6181 cggtgccagc atcagccagt ttgagctgca tctgcgggcg attaccgatc tgccgttacc
     6241 gcaaccggta gtgaatagtc cgtcggtgat gatcaacctg attggtagcg atgtgaatta
     6301 tgactggctg aaactgccgc tggtgcatct gcactggtac gacaaagaag tccgtccggg
     6361 gcgtaaagtg gggcatctga atttgaccga cagcgacaca tcgcgtctga ccgcgacgct
     6421 ggaagccttg atcccgctgc tgccgccgga gtatgccagc ggcgtgatgt gggcgcagag
     6481 taagttcagt taagcaggtg ctcatttgat tgccggatgc ggcgtaaacg ccttatccgg
     6541 cttaccgctc cggtacccat tgtaggcctg ataagacgcg tcaagcgtcg catcaggcat
     6601 ggtgcaccga ttgccggatg ctctcatcac tacagcgaaa tacaggtccc cgcttcgccc
     6661 gccagcgtct cttcaattcg cgataacgcc ccaatccacg cgggtttacc acggcttctg
     6721 acataaccac ttaccgccgt taccttcggc cccatcgaac catcggcttt ggtaaatggc
     6781 gctaactcat ccggtgtggc atggcgaatg gcgcgttgct gcggcgttcc ccagttttca
     6841 taaaccgcgt cagcatcggt gaggatcacc agtccatcgg cattaatctg ctcggcgagc
     6901 aacgcagcgg cgagatcttt atcaatcacc gcttcactcc ctgccccatc ttccgtcaca
     6961 ggcacaccgc cgccgccact gcaaatcacc acatgcccct ctttgagcaa caactcgatg
     7021 gcttcgctgt cgagaatttt acgcggttgc ggagacgcca ccacccggcg caaatattta
     7081 ccatcacgtt tcatctgcca gccgtaagcc gcttccagtg ccttttgttc ttctggctga
     7141 taaaccggac caataaattt ctctggctgc aaaaacgccg gatcatcagg cgaaacctca
     7201 atgcgcgtca gcaccgtcgt gacaggcggc atctgcggct gtgcgctcaa actctgcgcc
     7261 agcatatagc caatcatccc ctggctttcc gccaccagca catccagcgg atacggatct
     7321 acctctttcc acgccagatt ctgcaatgcc agcagcccca cctgcggccc attgccgtga
     7381 acaatcgcca gccgataaga acgggccagg cgcgccagcg cgggtacagc actggcgata
     7441 ttgcgatatt gattttctgc cgtcagcgcc tcaccgcgct ggagtaaggc gttgccccca
     7501 agagctacaa ccagtgtttt catggttttc cttgtaataa ttgttggcca agccagaacc
     7561 ccagcagcgt atctgcccct gacgtatgcc ccagcgccag cagcgaatca atcgcaacag
     7621 cggtacgctt cgggcaactc agagcatgga caaagtgcag gagtggcgag gcgaaatatc
     7681 cttgcgcggc ataacgtaaa taactgacgc tcaccgctgt ggtaacgagt tgaagattgt
     7741 cggaacaggc aaaaaacggg cgaccggcgc gcgcatctaa agcgccataa taccaggccg
     7801 ccagcagcat tccgctcaac gtgtcatcat gacttggcgt taatccgggg cctttaccca
     7861 gccagtgccg ccagtcggtc ttaacgccat tgagcgcggc ctgaaaacag tgacgaaact
     7921 ggcgtaattc agcgggcagc ggatcgcttg ccgccatcgc cagtggtccg aaaagtccgg
     7981 tttcctccgc gcgttgcatc catgcagctg caagcggttg aggatgcgaa ggcggcgtaa
     8041 tacgcagcaa acaataacgc tgtggctgtt taaccgtgaa acgcccgagg cgaatccctt
     8101 gagccacaac ctgtggtcgt tcattgccgc ataatccacc gcataacgca tcgaattgcg
     8161 cgcggcgaag cacccatcct ccggggccga aaccactacc ctgacgatgc agcgttaaca
     8221 gttcgccgct ttccgtcatc aggttaatcg cccgtcgcca cactcccgct aaccgccagg
     8281 actggcgata attcggtgca ctacggctgg caagcagagg atggatgatc gtcattgcgc
     8341 gttcctcaac caatccccat gctttcagcc agtgcttcca gcgcctgttc aaaacacgcc
     8401 agcggtgccc gcacagtgcc tgcgccaatc tgcccgatcc ccgcctcttt atgggcgata
     8461 ccggtattga tgagtggcgt aatgccggtt tctaccacgc gacgaatatc cagccccagg
     8521 cacgcgccct ggaaatccca gccggggatc tgcaactgca tattgcgctc aaggtaaatt
     8581 tccgccatct cttcagatac cgctctggcc gcttccatgc cacccgcacc gacaaagcgc
     8641 gttacgccag gcgctgcgat cattgccgca cctccgatac caaaggtttc ggtaatcgcg
     8701 ctatcgccca tatccgggtt cgcctgctcc tgcgagaagc cggtgaaaaa cagaccttgc
     8761 ggagtgttta caggcgcagt aaaccagcgt tcgcccagcc cgcttacccg aatcccgaac
     8821 atattgccgt tgcgggtcat tgccgtgacg atgctgcctg cgcggatcat cgcgccagca
     8881 tccatcgccg ccttgcagta agccatcgcg aggttgagga agaactgatc ggtcacgctg
     8941 aggaaatcca tcacttcggc gatgtgctgt ttatcatgat cgaggcgagc aatttgtggg
     9001 gccagcgcac gcattaacag tgcagaggaa gcaatattgc gttgatggaa ctcatcgccc
     9061 atcgtaatgc cctgcgccat catcgccgtg agatcgatac cgcgctccat gcgccccagc
     9121 gccgcgctta atactggcat taacacatcg cgcatccagc gatggcgagt caggacatct
     9181 tcgccgtaag cgccaaaacg catcactttg ccgatacctt cgttgaggtt gcagtacgcc
     9241 cggttgccgt cggtcacgtt ctcgaccacc agcatcggca tactggcaga agtaataccg
     9301 cccattggac cgacggcatt cacatggtga caaggaatga agttcacttc cccctgctcc
     9361 agtattgcca gcgcctgcgc ttcatctttc gcccagcctt cgaacagaca tgcgcccacg
     9421 cacgcccctt tcatgggtcc ggtcatctcc tgccagcgca ttggcggccc ggcgtgaagc
     9481 agtgttttgc cctcgtttag ttcgctgata agtgaagaag ccggttgcac atccagccag
     9541 tgtggacgag cacgacgaat ttgttcgata accgcagcat tggcttgcgc cactgatgta
     9601 aacatgggaa ccccttattg caaacgttct aataaacgag ccagtttttt attgccgccg
     9661 gcgactggcg accattggta atgcacaacc ggtttgctgg cgctttgtag ctccagcgca
     9721 aagctgcgta atccgatgtt aatcacggcg acgttttcca gtaatgacgg tgtgtgttgc
     9781 tgtgtggcag acgagagcgg atgaattaac gctgccgcca gcaaggtggc ttccggtagc
     9841 gaactcacta ccgcgatccc cgcatcttcc agcgtggcga tttgctgcga gcggcattgc
     9901 gggtcacgtt cggtacctgt caccgtggca atggcgtaca gcggttggct gtctgaacgc
     9961 gcggcacagg ctttttgcca ggcgctcacc agcgaggcag caggatcggc ggtcgcaccg
    10021 aagccaatca cgacatcaag cagcaacacg cgcacttgcg gtttagcgcc gagatcggca
    10081 attaactggt tgcgtaaggc tgggtcgatc atcggatggg gacgcccgac ggtgtagaaa
    10141 tcatcgccca gatcaatgat ctggtgacca tcggcgtcca gcatcatgcc gtgatgatgg
    10201 gtatcgtcgg cttccacgcc aaggtgcccg gcaagtaatc ccgccgcttc ggcagcaagc
    10261 gtaccgccgg tatacaaacc gcaaataaat ccgctgctga caggcgctat tgcgttacgt
    10321 cgcgccgtga cgcgtgaaag caggcaagcc aggcgtgcgg cctcatccag cgaggaggca
    10381 aaccagacat tctcgtcgcg ggccaccgcc ggggtataac ctaaaaacag cgccaccgtc
    10441 ggtttgccgg ttgctttcat ggcattaaca attttcagac gcacagcttc ggcaggtggt
    10501 tttgaaacaa atgccagcac ttcgcttttc tcgtctgcac tgagcatttc cagcgctgtt
    10561 agcgcactga tgccgcccac ttcacggctg aggtcgcgcc cgccaaggcc aatcgcgtga
    10621 gtaattccct cccctgccag cgcaatctgc gaacacagct cctgaatccc ggtaccggaa
    10681 gcgccaatga cgccaatatt gccttctggc atcacgttag caaaagccag cggtgtgccg
    10741 gcaatcatcg acgtaccgca gtccggcccc atcaccagca agcctttttc ccgcgcgcgg
    10801 gttttaagtt ggatttcatc ttccagcgtg acgttatcgg agaacatcat cacgttgagg
    10861 ttgcgatcca gcgcctggtt tgtcagctcc gccgcatact cgccagccac tgaaatcagc
    10921 gccagattgg catcgggtaa tttctgacag gcgctgtccc agcgacgcac ctgcgtcaac
    10981 gcctggctgc tgcctgaccc ctgcgccagt tgttttagcg cctcttcaag ctgctgcata
    11041 atcgcctgcg cgatccccgc atccgccgct tcgctacgaa ttgccacgca aatatcgttc
    11101 ggcgtggcgt tattaaaatc gtcatgccag aaacctgtgg tatctaataa cgctttattg
    11161 gcgggcgtac ccatcattac ggaaacatca tcaacatttt ctgattcgct gagttttcgt
    11221 gaaataatca ttaaactgac cgaatcctga aaacaccctt ttttaataaa ggcgtggatc
    11281 ataacaactc cttaaaaatt gcacccatgg caattgcacg agcagtgtta atgtggcgat
    11341 tacggtatcg atgacaacga gtaaataagg tgatgaatat cacacaggga ttattaacaa
    11401 aagaattcaa ataaccgcca ggctattacg cattattttt aataagcgtg gttgttttaa
    11461 aaatcaggtt aaaagcgcga agattatcaa ataaattcta actatatttt ttgcctgtct
    11521 ggatcacata atccagatat tttccctgta tgttaattgc agtcatgctt cacaccgtcg
    11581 ttaaaaagga agacagatga aaatcagtcg ggaaacactc caccagctaa ttgagaataa
    11641 actctgccag gctgggttaa aacgtgagca cgctgcaacc gtggctgaag tattggttta
    11701 cgccgatgcc agagggatcc actctcatgg cgcggtgcgc gtggaatact acgcggaacg
    11761 catttcaaaa ggcggcacca accgcgaacc ggagtttcgt cttgaggaaa ccgggccgtg
    11821 ctcggcaatt ttacatgccg acaatgccgc cggacaggtc gcggcgaaaa tgggtatgga
    11881 acatgccatc aaaaccgccc agcaaaatgg cgttgcggtg gtcggtatca gccggatggg
    11941 tcacagcggc gcaatctctt attttgtgca gcaggcagcc cgcgccggat taattggtat
    12001 ttcgatgtgc cagtccgatc caatggtggt gccgtttggc ggcgcggaaa tttactacgg
    12061 tactaaccca ctggcctttg ccgcgccggg agaaggcgac gagatcctta cctttgatat
    12121 ggcgactacc gtacaggcat ggggaaaagt gctcgacgcc cgctcgcgta atatgtctat
    12181 cccggatacc tgggcggtcg ataaaaacgg tgcaccaaca accgatccgt tcgcggtaca
    12241 tgctctgctc cccgccgccg ggccaaaagg gtatggcctg atgatgatga ttgacgtcct
    12301 ctcaggcgtc ttactcggct taccgttcgg gcgacaggtt agttcgatgt atgacgattt
    12361 acacgccggg cgtaatttgg ggcaattaca tatcgttatt aacccgaact ttttcttctc
    12421 cagcaaatta tttcgtcaac atcttagcca gaccatgcgc gaattaaatg ccattacccc
    12481 cgcgcccggt tttaatcagg tttattatcc cggacaggat caggatatta aacaacgcaa
    12541 agccgccgtc gaaggcatcg aaattgttga tgatatttac cagtatttaa tttccgacgc
    12601 gctttataac acgtcatacg aaacgaaaaa tccctttgcg caataattat tgagacagga
    12661 ctttcttatg attacacatt ttcgtcaagc catagaagaa acgctgccct ggctttcctc
    12721 ttttggcgct gacccaacgg gtggtatgac ccgtttactt tattcgccgg aatggctgga
    12781 aactcagcag caatttaaaa aaagaatggc agcaagcggg ctggaaacac gtttcgatga
    12841 agtggggaat ttatatggtc gcctgagtgg caccgaatat ccacaagaag tggttctgag
    12901 cggttcgcat atcgataccg tggttaacgg cggtaacctc gacgggcaat tcggcgcgct
    12961 ggcggcgtgg ctggcaattg actggctgaa aacgcaatac ggcgcgccgc tacgtacggt
    13021 cgaagtggtg gcgatggcag aagaagaagg cagccgcttc ccgtatgtct tctggggcag
    13081 taaaaatatc tttgggctgg cgaatcctga cgacgtgcgg aatatctgtg atgccaaagg
    13141 aaatagtttt gtcgatgcga tgaaggcttg cggatttact cttccgaacg ccccactaac
    13201 tccgcgtcag gatattaaag cctttgttga actgcatatt gaacagggct gtgtgctgga
    13261 aagtaatggg caatcaattg gcgtggtgaa tgcaattgtc gggcagcgtc gttatacggt
    13321 aacgctgaac ggcgaatcaa accatgcagg caccacgccg atgggttatc gtcgtgatac
    13381 agtttacgct ttcagtcgca tttgccatca gtcggtcgaa aaagcgaaaa ggatgggcga
    13441 tccgctggtt ctgacctttg gcaaagtaga gccgcgcccg aatacggtaa atgtggtgcc
    13501 gggtaaaacc acgttcacca ttgattgtcg tcataccgac gctgccgtgc tgcgcgattt
    13561 cacccaacag ttagaaaacg acatgcgggc gatttgcgat gaaatggaca ttggtattga
    13621 tatcgattta tggatggacg aagaacccgt gccgatgaat aaggagctgg tcgccaccct
    13681 gacagaattg tgtgaaagag aaaaactgaa ttaccgggtg atgcacagtg gtgccgggca
    13741 cgacgcgcaa attttcgcgc ctcgcgtacc aacctgcatg attttcattc ccagcatcaa
    13801 cgggatcagc cataacccgg cggaacgcac caatattacc gaccttgccg aaggggtcaa
    13861 aacgttggca ctcatgcttt atcaacttgc ctggcagaaa taaggagtca taaatgggat
    13921 atttaaataa cgtcaccggt taccgcgatg atttactggc taaccgtgcg attgttaaac
    13981 acggtaattt cgcactgtta accccggatg gtctggtaaa aaatattatt ccgggctttg
    14041 aaaattgtga cgcgacaatc ctctccacgc caaagctggg tgcctctttt gttgattatc
    14101 tggtcacact gcatcaaaac ggtggcaacc aacagggctt cggtggcgaa ggcattgaaa
    14161 cgttcctgta tgtgatctct ggaaatatca ctgccaaagc cgaaggcaaa acatttgcct
    14221 taagcgaagg tggctatctt tattgcccac caggctcctt aatgacgttt gttaacgccc
    14281 aggccgaaga cagccaaatc tttttatata agcgccgcta tgttccggta gaaggccatg
    14341 caccgtggct ggtttctggt aatgccagcg aactggaacg cattcactat gaaggtatgg
    14401 atgatgtgat cctgctggat ttcctgccca aagagttagg ttttgatatg aacatgcata
    14461 tcctctcttt tgcgccaggt gccagccacg gttatatcga aacacacgtt caggaacatg
    14521 gtgcctatat tctttccggt cagggggttt ataacctcga caataactgg atcccggtga
    14581 aaaaaggcga ttacatcttt atgggcgctt attctttaca ggctggttat ggcgtagggc
    14641 gtggtgaagc gttcagctat atttactcga aagattgcaa ccgcgacgta gagatttaag
    14701 tcataccact cgcctgatta tttttagcta tgttggattt tgccacggtt tataccgtgg
    14761 ctttttttgt agtaaattca ggtaaaaaaa gatgcgaaat aaatcgcatc cgctttaaag
    14821 gtaagggtca gtttttaatt ccctgaccta ttttaatggc gcaggcaata tttcgcgcgc
    14881 tgttgaagag attggtttca ccgctggcga gcacttcggc taaaggtgcc agacgaggca
    14941 aaatgctgaa taccgcgtca atgccgtact ggtgcaccac ttccacgcca tcacccaata
    15001 cgccagcaat cccaatcacc gggacattaa actgcttcgc caccgacgca acgcccagcg
    15061 gcgctttacc gcctgccgtt tgcgagtcaa tgcgcccttc cccggtgatc accagtgctg
    15121 cgccctgcac cgcctgctcc aggttgaccg cccgcagtac aatctcaata cccggtttaa
    15181 tatccgcatt gaggaatacc gccgccgcaa tacccatacc gcccgcagcg ccgccgcccg
    15241 ccatctggca gacattaatt tcagtttgct gttgtaaaac acgggcgtaa ttttgcagcc
    15301 cctgttcaag ttcttcgacc atctccggcg ttgccccttt ttgcgggcca aacaccgccg
    15361 ccgcgccgcg tgcccctacc agcgggttat caacgtcaca tgctacttca atatggcaat
    15421 tcgccagacg cggatcgcat tcatccatct caatgcttgc cacgcgcgct aaattaccgc
    15481 cgttagccgc cagcacctgg ccgtcggcat caaggaaacg cacaccaagc gcctgagcca
    15541 tgcccatacc gccgtcgacc gtcgcactgc cgccaatgcc gagaataata tggcgaatgc
    15601 cgttatccag cgcatgacga attaactccc ccgtaccaaa actggaggcc agcaacggat
    15661 tacgcttttc aggcgcgacc agcatcaggc cacttgctgc cgccatctca atcaccgccg
    15721 ttttcccgtc gccggtaagg ccataaaaag cattcactgt ttcgcccatc ggcccgcaga
    15781 cttcaagcgt cacgatgttg ccgcccgtcg cggcgaccat cgcatccacc gtcccttcgc
    15841 cgccatccgc tatcggcaaa cagatatagt tcgcatcggg aaagagggtc gaaaacccgg
    15901 ctttaattgc ctgacaacat ttttctgcac ttaagctctc tttaaaagag tctggcgcaa
    15961 tgacaatctt cattatcaat cctttttcaa caaattattc gatgcgttcc cacggaataa
    16021 gattatccat aagaacagcc agtaaaattc cgaccagtaa gccgttactg agcagaggac
    16081 gcagcgttaa tggaaggtct tgcagataca caggcggtaa tgccattaaa aatatgccga
    16141 caaataacgg caatgcgagt cgataaatat tgcgagcggt aaaggttatt tgctggctaa
    16201 acaccagcgc ggaaaagagt aaaggcagat aagaaaccag catgaccgcg ctactcacgg
    16261 gtaaagggat actgcaaaac agtcgtgtga gtgccggaac cagcgccacc agcaggcaaa
    16321 taacgctgcc ataaataaac gaacgccgcg tgtaatcgcc tgtctgggtt aataaaccaa
    16381 tggatgaaac aaacggtgaa aatggaatta ccgccagcgg tacggttatc agcgtcataa
    16441 atccggtcgc cacaaagctg cgacgataac gcgtattccc gcgccctgct gcggataaaa
    16501 aacatccgtg ccccgaatcg caccgtaggt attgctgata ttcaccagac ctgtaatcac
    16561 cgccgtcaga ataattcccg gagacaaagc acctccactg ccgagcggga accactgcca
    16621 gtgcaactca ccggagagcg agtgcgaaga aggaaagcaa aagtaccaca acaaccagcc
    16681 ggttatggtg ccgaccagca ggccataacg ggcaaaacgt tgcggcagga agataatcat
    16741 ccccagtacc aggcacatca ccgccaccga gagcgcgaac ggcggtaact gaattttaaa
    16801 atttgggtcg gctatgccaa acggcagccc gagcatacct ttgaaaaaga tagtggtcag
    16861 ctgcgcgccc agcatcaaca taaacaagac catcaccgac ggcgtaaata accgtgccag
    16921 gcgatggcct aatccgctaa aaccaatcaa catcgtcagc acgccggaga gcgcaatccc
    16981 caccgccagg ctggtagcga tatcgttgat cggtgtcccg cgcgatgctt cgccaagggt
    17041 gatagtaagg atggttcccc accacaggcc acccggccct tccataatag cgcgacgatg
    17101 accgcagaac gcctgagcga agcaggccag tgcggtagca agaaaagcat attgcgtgag
    17161 cgtaagcagg ctactttgcg gcaactgaaa agcagaaagt agcgtaggag gaaccacaac
    17221 cgtgttgcaa aaaataaaga aaaaccactg aaatcctgat aacaggcttt cgcggctcac
    17281 tgcaaaattg aacataaaca caccttaacc ggagaatgcc cgccgcaagg acgggcattg
    17341 caggggccag attactgctg atgtttaagg ataaattgac ctttcggcgc aacagggaag
    17401 ccctgttcaa tgtcgtaaat cacatcacca cgtaagatgg ttttcgtgat acgcgcgcca
    17461 atggtacggc caacatacgg gctgactttg tggcgatatt ccagatcgtc attggtaaga
    17521 acatagctgc tattcggctg aatgaagacg aagtcggcat cttttcctgg ggcgatacgg
    17581 cctttttgct gcagaccgaa aatatctgct gcgttagtcg ccattaattt gccgaacatt
    17641 ggcagagaca ttccgcgttt ctgtaccgct tcatcgaaca tcacgtccat acagttttgc
    17701 agaccggcaa taccgcccca tgcttccatg atgttgccgg ctttcatttc cggagggcat
    17761 ggtgagtggt cggaaaccag gcagtcgatt tcaccgttaa acagtttttc ccacatgcct
    17821 ttctggtttt ccagatcgcg gatcggcggt gaacacttcg ccagagtgcc gatttcttcg
    17881 aactgatcgg tatccagtac aaagtaatgc gggcaggatt cacaagtaac gtcctgacct
    17941 tcctgacgtg cacgagtcac ttcttcaacg ccttccgggc tgctgatatg gcaaacgtgc
    18001 agacggcaac cggcaacttt cgccagatac agcacgcggc gaatcgcttc cacttcggta
    18061 aataccggac gcgaagccac atagtcatgg gcagttacgc gaccttcacg tttcgcttct
    18121 tcgcccagtg cgtcacagat cagcgcgttt tcgcagtgca ccagcaccgg ctgccccagt
    18181 tcgcccagct tctgcgcacc tttgaagaac tgccagtcgt ttacatcacg gaagtcgttg
    18241 tcgataccgc gatcgccaca ggtcgcaacg aagcatttga agccgacaac gcccacttca
    18301 tccagctcat gcagacggtc gatgttgtaa gacaccaggc caccgagttg tgccgcatcg
    18361 atagtcagct tgcctttagc ggcatcgaac ttcagttcaa ttgaagcgcg gtcaaccgtt
    18421 gcaggcagct ggttgagcgg catttcgatc atggtggtga taccaccttt tgccgctgcg
    18481 cgagtaccgg tttcataacc ttcccagtgg ctacgacccg gttcagaaat atgggtgtgc
    18541 gcatcaacca tgcccggcga aaccaccaga ccagacgcat ccataacttc ttttgcatcg
    18601 cccagatcct gaccgatagc agcaattttc ccgcctttaa cggcgatatc tacaacgcga
    18661 gcttcgtttt ctaaaataac ggtgccgttt ttaatgatta aatcaaaaga cataacaaac
    18721 tccttgtaaa atacagattg gtttttaact caatgttcaa tatcgggatt aattaaccta
    18781 tggttttttg ctctcctgtt ttttctgctg ttgtacgttt ctttaataag gcgtaggccg
    18841 caaaggcgac gatgacgccg acaaaccatg aaacacgcga taacggttcc ataaagtgaa
    18901 taaacttacc gccaagagat aaaataacgg ccaccagagt tactgaaaac gcagtgaggt
    18961 taaaaccgtt atcgtaatat ttataatcgc caggtgcggt atacagttca tcaagattaa
    19021 tttgtccgcg catcaccaca aaataatgcg ccatcatgac accaattacc ggaccaagca
    19081 ttccgccgat aatatcgagg aaaagataaa tgctgtcctg attttccatt aatttccacg
    19141 ggcagatcag caagctgata atactggcaa tcagtacgcc gtttttatag gtcagttttg
    19201 tcggtgcaat ggcggcaatc tgataaccgg ctggaataat attaccggtc gcgttagtgg
    19261 agatagttgt catcagaatg accagtaccg caaagaacga ggcgaacagg ctgtcccaac
    19321 gctgaacaat atccagcacg ttccaggtat cagcgccgta gtgaatactg gctccggcaa
    19381 taatacatac cccggcgacc gcaaacagaa tataggccac aactaaaccc agcgtttgcc
    19441 ccagcgcctg ctcacgaaac gagtgggcgt tttgcgtaaa gtcggatgcg ctcaccgccg
    19501 gtgccgccca gaccgcaact accgcgttaa tcaccaccag gaacaggaag ccaccgtttt
    19561 ctgctttctg aataccgctc ggaatgtagt caaagattgg accgatcccg accagtgaaa
    19621 tcgcccaaat cgccataccg ccgaaaacga tatagatgca cgggttaaga atggcagtga
    19681 atttatttaa aactttgcca ccgccaaaac ctataccaac gttgaccagc cagaagatta
    19741 agaaagtaat taagcccggt agagaaaggc ctaacagagt gaaatcacca ccgagagtta
    19801 aaaatcccgg ccagattttg ccaatcagaa tcaagcaggc cagtgacccc gcgtaacatt
    19861 gcaaaccaaa ccacatgatg gcggcaattc cgccccttaa taatccggga aacagtgcac
    19921 cacgtacacc gtaagaagca cgcaggatca tggcaaaagg cacaccgtat ttactgcccg
    19981 cagcaccgtt taataccatt accgcggcaa tgaaaaaggc gctgaggata attgccagca
    20041 taatactaaa ggtagacaag ccgagaataa aaaagccgcc gaccatcaca taattgggaa
    20101 cgttatgaac cgaacccatc cataaggtaa aatagttaaa tgttttccag gtccgctggc
    20161 tttgcgtttt cggcaataga tcttcgctat agccgcgttg ctggaatagt tttctctgat
    20221 gttccataaa tccctcttaa tttcacgtat cgaataacgt tgttttaatc tagctatgag
    20281 gaacaaataa aataaaaata ttatttttaa tactgaagca acatctttgg ctgctttctt
    20341 ttttaatatt gctggccgat tcattttctg atatcggcca tttttattgc gggtatcagg
    20401 ccagtttatg gttagccatt aattccagcg cctgcactaa cgcagagtga tccaactggc
    20461 tgccaccgtt tgccgcgcag gtattaaata actcctggca ggtcgcagtg tttggcaggt
    20521 tcagcgctag cgctttcgca ctttgcagtg ccaggttgag atctttctgg tgcagcgcaa
    20581 ttttgaaacc tggattaaaa gtgcgtttaa tcatgcgctc accgtgaact tccagaatac
    20641 gtgaggaagc aaagccgccc atcagcgcct ggcgcacacg taccgggtcc gcaccggctt
    20701 ttgaagcaaa tagcagggct tcagaaaccg cttcaatatt gagcgccaca ataatctgat
    20761 ttgccacttt gcaggtttga ccatcgccgt taccgcccac gagggtgata tttttaccga
    20821 gcagttcaaa cagcggttta acacgttcaa ataccgcttc atcaccgcca accataatcg
    20881 acaacgtccc ttcacgcgca ccgatttcac cgccggagac tggcgcatcg agataatcgc
    20941 cgcccagttc attcacctga cgagcgaaac gcttagtttc aatcggggaa atggagctca
    21001 tatcaacaat ggttttgccc ttcagcgagg ctttggtaca accattttca ccgaacagaa
    21061 cttcttcaac ctgtggtgta tctggcacca taataaaaat gatgtccgat gcttccgtta
    21121 cctggcgcgc agtttcaaca ctgacggcac ccagtgacag taattcatca gcaaccggac
    21181 caatggttgt gacatgcagt tgatgaccgg cacgcgccag attaatggcc atcggtgtac
    21241 ccataatgcc taagccaata aatcccagtt tcataattaa cctcttttaa atttcgcttt
    21301 tcctgaattc agacaacacg atcccctgcc aacttagcgg cctaaaaaag cattgcctga
    21361 acagcgttac gttttaacgg tacggatcca tccagcgtaa accggcttcc gtggtggttt
    21421 ggggtgtata ttcacaccca acccaaccgt tgtagtcaga attttcgatt accttaaaga
    21481 gataatcata attaatttct ccggtccccg gttcgccgcg atgcggatta tcagcaattt
    21541 gcaggtgacc aattttatcg gcccactgag tcatggtgtt ggttaattca ccttccatcc
    21601 gctgcatgtg ataaatgtca tactgaattt tcaaattgca gcaaccaaca tcatcaatca
    21661 atttcagcgc ctgccgagtt ccggtgagat ggaaaccagg aatatcaaaa tggttaatag
    21721 gttcaatcag taataaaata tcttctttca tcagcatatt cgcggcataa cgcaggtttt
    21781 ctacaagcgt tgcgtgaatc tgttcactgc tgaaaccagc cggcgtttta ccgaccagac
    21841 agttaatttt tttattaccc agcgcacggg cataacgaat cgctgctgct acgccatccc
    21901 gaaactcttc ttcacggcca ggaatacagg caataccgcg ctcccccgcc gcccagtcac
    21961 ctgccggtaa attgtgcagc gtatgttcga gtttattact cgccagcacc tgttttaatt
    22021 cttcaatgtc gtagtcataa ggaaacataa attcaacgcc gcgaaaacca cactgcgcag
    22081 ctttctcaaa acgggcgaga aaatcatatt caccaaataa catcgataaa ttagcagaga
    22141 aacgtaacat tatttatctc ccttattcat agtgcatgaa gcaggtttca gtcggtgcgt
    22201 ccgctgcgtt atcggcgata tcttcaaatt ccatgacgtt atccagttcg ctgcccatcg
    22261 aaatattggt cacacgctca agaataactt ccacgactac cggtacccga tattgcgcca
    22321 ttaaggcttt cgcctgttca aaggctggcg caatatcttc cggtttgaag acccgaatag
    22381 ctttacaacc taaaccttcc gctactttta cgtggtcgac gccgtaaccg ttcacttcac
    22441 tggagttaat attttcgaac gcgagctgta cgcagtaatc catgtcaaag gcgcgttgtg
    22501 actgacgaat cagccccaga taagcgttat tgaccagtac atggatgtac ggaatgttga
    22561 actgtgcgcc aacggctaat tcttcaatca ggaactggaa gtcgaagtca ccggaaatcg
    22621 ccaccacatt ccgttctgga tcagcagcac acacgcccag cgccgccgga atggtccagc
    22681 ccagcgggcc tgcctgacca cagttgatcc agtgacggtc tttaaagaca tgcagcattt
    22741 gtgccgcagc gatttgtgac aggccaatgg tggtgacata acaaacatcg cgaccaaagg
    22801 ctttgttcat ctcttcatac acgcgctgcg gtttcaccgg cacgttgtcg aagtgggttt
    22861 tgcgcagcaa agtgcgtttg cgctgctggc agtcggcgac ccattcttta cgacacggca
    22921 gacgacccgc tttttgcatc tcctgcgcca cttcaaccag cagtgtcagc gccgctttag
    22981 catcagagac aatgccgaga tccggacaca gcacgcgacc aatttgcgtc ggctcaatat
    23041 cgatatgaac gattttgcgc ccttcggtgt atttctctac cgaaccggta tgacggttag
    23101 caaaacggtt accgatacca aacaccatgt cggacgccag cagcgttgcg ttaccgtaac
    23161 gatgcgcggt ttgcagaccc accatcccgg ccatcagttc atgatcgtcc gggatacagc
    23221 cccagcccat cagcgttggg atcaccggaa cgctggtcag ttcagcaaac tgttgtaaca
    23281 gtgcagctgc gtcagcatta attaccccgc ccccggcaac aatcaccgga cgttcggcct
    23341 ggattaacat ttctacagct ttttcgatct gcatacggct ggcagcaggt ttgtagaccg
    23401 gcagcggttc gtacatatca ggatcaaact cgatttccgc aacctgaacg tcgaacggta
    23461 aatccaccag taccggaccc ggacgaccag aacgcatcag gtgaaatgcc tgttgcaaca
    23521 cacgaggcac cagcgccgct tcacgtactg taaccgccat tttacttacc ggcttggcga
    23581 tagcttcgat atcaacagcc tggaaatctt ctttatgcag acgggcgcgc ggagcctggc
    23641 cagtaataca cagaatagga atggaatcag cagaagcaga atagagcgcg gtgatcatgt
    23701 ccgtgcccgc aggaccggaa gtccccagac atacgccgat attccctgcc gttgcgcggg
    23761 tataaccttc cgccatgtgc gaagcacctt ccacatgacg cgccagaatg tgacgaatac
    23821 cgccgtgctt acgcatcgct gagtagaacg gattgattgc agctcccgga acgccgaagg
    23881 cggtggtgat accttctttc tccagcacat acattgccgc gtcaacggct ctcatttttg
    23941 ccattttatt ccaacctcta ttaattggaa aaattttcca actttcaaat tttgtcaaaa
    24001 aaacccgctc cgcagagcgg gatgcgacag acactatgga tgtgctttca gtcccaacgc
    24061 cgtgctgata tcgcgggcgg tgtctctgac cagctcaccc tgactgacaa aacgatcttc
    24121 tgtcagtctt gatgaaggcc cggagatgga gatagcggca acaacgctac cgacatcatc
    24181 gtaaattgct gaagctatgc aattcagacc tacaacatgc tcttctttat ctacggtata
    24241 gcccagttca cgcgcttgtt ccaggtcctt cagcaaggtg ggcatatcca caagcgtagt
    24301 tggcgtaaac tgctgcaaac cggtttgcag aatgatgctc atcaactcct cttccgccag
    24361 cggataaagc aacgccttgc ccgcaccgga agcatgcagc ggcagacgac tgcccagtgg
    24421 cgcacacatc ctgaccatcg atttacactc taactgacca attaataccg cttcattgcc
    24481 gttacggatc gcgacattga ccgtttcgcc ggaaagtaac atcaggcggc gcataaacgg
    24541 cccggcgacg gagaggacat cgcggttatg gatgtacgcc gcaccgacgt taaagacacc
    24601 taatcctata tgccaccagc ctaactgact gtcctgatag acaaaatccg ctgcctgtaa
    24661 aaccttcagc aagcgaaagg tcgtagagag cggcaaatcc agattgagag aaatatcgct
    24721 aaccgacgaa cttcccccac ttttttccag atattgcaga atcgcaattc cccgctctaa
    24781 cgcctgtgcg cccttctgtg ccacaggctc cgcctgtcct ggcctgccgc gccgtctaac
    24841 ttccgtcata cctacgttaa ctccgaccta caagtgagca taaacaatga acaattagtt
    24901 tatcttatgc aagtcggttg caggcgtcac gcaaaacaga gttcctgttc aggaatactt
    24961 tcaacatcac agttgtcact gccgccgcga tcgatggtca gaaaatcggt gacgcgctgc
    25021 caggcgaaaa gtgggtgatg ccagacgtta cggtggtaat tcactccctg ttcgccgttg
    25081 gtaataaacg cccgcagggt tgacaggtct ggtttgtcgt cacctaacgc cacgaccacc
    25141 acaaacacct cacctttcat cgggataaag gcctgagtac ccagcggatg acgttcgagt
    25201 tcgtgaatgg tcagcggcag attcgccggt tgcgcgcggt taatgctgat aagcgtacgg
    25261 tcttgctcaa gaatctcaac cagcgccaaa tcgtggtaac gctccaccag gccattgtta
    25321 atatggaaaa aatcccgttg ctgcgtttcg attacgtcgc cataagcact aaaggcttcc
    25381 tgacttaaca gtaatacctg aagtttcatc tttttgtcct tgtaacataa ttccgttcaa
    25441 ccaccgccga ccggtcagct tggtttcaat aacaggcaat ctaaaacctt aagagaacaa
    25501 catcaataca aaagttggaa tatttttcca aaactagatt tagtccacaa attgaacagg
    25561 gttatcatgt aaaaatactg ataaatcaga ggataagaaa atcacgcaac ttgcggattt
    25621 aaaaggggat acctgaagat gttcgatcca gaaaccttgc ggactttcat tgcggttgct
    25681 gaaacaggaa gtttttcaaa agcggcagaa cgattatgta aaaccacggc gacgatcagt
    25741 tatcgcatta aacttctgga agagaatacc ggagtagcgc tgtttttccg cacgactcgc
    25801 agcgtgacgt tgacagcggc tggcgagcat ctactttccc aggccagaga ctggctgagc
    25861 tggctggaaa gtatgccaag cgagctgcaa caggtgaatg atggcgtgga acgccaggtg
    25921 aatattgtca tcaacaacct gctctacaac ccccaggccg tcgcccagtt gctggcgtgg
    25981 ctgaatgaac gttacccctt tacccagttt cacatctccc gacaaatcta tatgggcgtc
    26041 tgggattcgc tattatacga aggtttttca ctggctatcg gcgtcacggg aaccgaggcg
    26101 ctggcaaata cctttagtct tgatccctta ggatcggtgc aatggcgctt tgtcatggcg
    26161 gcggatcatc cgctggcgaa cgttgaagag ccgctaacag aagcgcagtt gcggcgcttt
    26221 ccggcggtca atattgaaga cagcgcccgc accttaacca aacgcgtcgc ctggcgattg
    26281 cctgggcaaa aagagattat tgttcctgat atggaaacga aaatcgccgc ccatcttgcg
    26341 ggcgttggca ttggtttttt gccaaaatcg ctttgccagt caatgatcga taatcaacaa
    26401 ctagttagcc gggtaatccc aacgatgcgc cctccttcgc cattgagtct ggcatggcgc
    26461 aaatttggca gcggcaaagc ggtagaagat attgtgacct tgtttaccca gcgcaggccg
    26521 gaaatcagcg gatttttaga aattttcggc aacccacgca gttaaaaatc aagtttcctt
    26581 gcgcgttggc gcgtaaaata gcgcgcaagc tgatttgagg ttcactttta actatgcaag
    26641 agagacacac ggaacaggac tatcgtgccc tgctgattgc tgatacgccc attattgatg
    26701 ttcgcgcccc tatcgagttt gagcaaggcg caatgcccgc cgctatcaat ctgccgttaa
    26761 tgaataacga tgaacgcgcc gccgttggca cctgctataa acagcaaggc tcagacgcag
    26821 cgctggcgct ggggcataaa ctggtggcgg gtgaaattcg tcagcagcgc atggacgcct
    26881 ggcgggcagc gtgcctgcaa aatccgcaag gtattctctg ctgcgcccgt ggcggtcagc
    26941 gctcacatat tgtgcaaagc tggttgtatg cagcggggat tgattatccg ctggtggaag
    27001 gcggttataa ggcactgcgc cagaccgcga ttcaggcgac tattgaactg gcacaaaaac
    27061 cgatagtgct gattggcggt tgtactggca gcggtaaaac gctgttagtg cagcaacagc
    27121 cgaacggtgt tgatctggaa gggctggcgc gccatcgcgg ttcggcgttt ggtcgcacgt
    27181 tacaaccaca acttagccag gcgagttttg aaaacctgct ggctgccgaa atgctgaaaa
    27241 ccgacgcccg tcaggatttg cgcctgtggg tgctggaaga cgaaagccgg atgatcggtt
    27301 cgaatcacct gccggaatgc ctgcgcgagc gaatgactca ggcggcgatt gcggtggtag
    27361 aagatccgtt tgagatccgt cttgagcgcc tgaacgaaga gtatttcttg cgtatgcatc
    27421 atgattttac ccacgcttat ggcgacgaac agggctggca ggaatattgc gaatacctgc
    27481 atcacggact ttcggcgatt aagcgtcggc tggggttaca gcgctataac gaactcgctg
    27541 cccagctgga tacggcgctg acaacgcaac tcaccaccgg cagcaccgac ggtcatctgg
    27601 cctggctggt gccgttactt aaagaatatt acgacccaat gtatcgctat cagcttgaga
    27661 aaaaagcgga aaaagttgtc tttcgcggtg agtgggcgga agtggcggaa tgggttaagg
    27721 cgcggtaatg agggacgtat ttgtattagt cgcgacttga tctgacacat ggccttgaaa
    27781 ggttgagagt taccggtttt gatatgagtg tcgaatcctt atccaaaaca agaggtaact
    27841 ctcatgcttc atactgccaa tcccgtcatc aaacacaaag ccggtttgct caatctggct
    27901 gaagaactca gcaacgtatc aaaagcctgt aaaatcatgg gcgtttcgcg tgatacgttt
    27961 tatcgttacc gcgaactggt cgctgaaggc ggtgtggatg cgcagattaa tcgtagtcgc
    28021 cgcgctccta accttaagaa ccgtaccgat gaggcaactg aacaggctgt tgttgattac
    28081 gccgtcgctt tcccgacaca tggtcagcac cgggccagca acgagctgcg taaacagggg
    28141 gtttttatct ccgacagtgg tgtccgttct gtctggttac tccataacct tgagaatctc
    28201 aaaagacgat actgaacaaa ttttatcagg tgacgttccg taaaaagttg tatggagttc
    28261 tcgatgcagt cggcgcgact atcagtaccc ccgctgaacc ctggactatc gggccgccgt
    28321 actggggcat gtcaccctgg cgtgccgctg gttttccgct aatggtttat ttcctctttc
    28381 tttcccggca agaatttatt ttaatccgga aggattattt attttcagat agggactcac
    28441 attaaacacg taacatttta attaatgcat ggctgaatat gtattacaca atgtcagata
    28501 tcgtaaaaga ccagtgcaat tctatgtaaa ctctctgact gttcatttta tttgttgttt
    28561 cagggtcgga actcaccctc aataaacaac tgaatacgag tagatattca tcgtctgagc
    28621 tatatggctt tacacaatag ccgacatgtc ttttctcagt caggaatttt acttttcctc
    28681 actacgaagt tgacgacttc gatatgggat agactcttaa ttcaagcaat tactctgcac
    28741 aagcatcagg caatccagct ccaatatttg ttataaaata atgcgtaaca ttaatttcac
    28801 caacaaaaaa aaggtttaca ccatcccctt gccctggtat cttaccatat ttacaccaca
    28861 ctttttttgt atctggatca ttctttgata cgattttcgc cacttgttcc ctggccctca
    28921 ctgtcaatgt tttcttaaac ctac
//
LOCUS       DAZFWL020000050        28392 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFWL020000050 DAZFWL020000000
VERSION     DAZFWL020000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.050"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..729
                     /gene="ybjI"
                     /locus_tag="ACHKNP_RS11730"
                     /old_locus_tag="pgaptmp_002346"
     CDS             <1..729
                     /gene="ybjI"
                     /locus_tag="ACHKNP_RS11730"
                     /old_locus_tag="pgaptmp_002346"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HGU4704500.1"
                     /translation="YQQMKAQGIRFVVASGNQYYQLISFFPEIANEIAFVAENGGWVV
                     SEGKDVFNGELSKDAFATVVEHLLTRPEVEIIACGKNSAYTLKKYDDAMKTVAEMYYH
                     RLEYVDNFDNLEDIFFKFGLNLSDELIPQVQKALHEAIGDIMVPVHTGNGSIDLIIPG
                     VHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVVAAAKYRA
                     GSNNREGVLDVIDKVLKHEAPFDQ"
     gene            815..1099
                     /gene="ybjH"
                     /locus_tag="ACHKNP_RS11735"
                     /old_locus_tag="pgaptmp_002347"
     CDS             815..1099
                     /gene="ybjH"
                     /locus_tag="ACHKNP_RS11735"
                     /old_locus_tag="pgaptmp_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HGU4704501.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(1140..2372)
                     /gene="mdfA"
                     /locus_tag="ACHKNP_RS11740"
                     /old_locus_tag="pgaptmp_002348"
     CDS             complement(1140..2372)
                     /gene="mdfA"
                     /locus_tag="ACHKNP_RS11740"
                     /old_locus_tag="pgaptmp_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HGU4704502.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            2657..3253
                     /gene="ybjG"
                     /locus_tag="ACHKNP_RS11745"
                     /old_locus_tag="pgaptmp_002349"
     CDS             2657..3253
                     /gene="ybjG"
                     /locus_tag="ACHKNP_RS11745"
                     /old_locus_tag="pgaptmp_002349"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU4704503.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            3311..4069
                     /gene="deoR"
                     /locus_tag="ACHKNP_RS11750"
                     /old_locus_tag="pgaptmp_002350"
     CDS             3311..4069
                     /gene="deoR"
                     /locus_tag="ACHKNP_RS11750"
                     /old_locus_tag="pgaptmp_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HGU4704504.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(4116..5318)
                     /gene="dacC"
                     /locus_tag="ACHKNP_RS11755"
                     /old_locus_tag="pgaptmp_002351"
     CDS             complement(4116..5318)
                     /gene="dacC"
                     /locus_tag="ACHKNP_RS11755"
                     /old_locus_tag="pgaptmp_002351"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU4704505.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            5565..6191
                     /gene="gstB"
                     /locus_tag="ACHKNP_RS11760"
                     /old_locus_tag="pgaptmp_002352"
     CDS             5565..6191
                     /gene="gstB"
                     /locus_tag="ACHKNP_RS11760"
                     /old_locus_tag="pgaptmp_002352"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HGU4704506.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(6188..7303)
                     /gene="yliI"
                     /locus_tag="ACHKNP_RS11765"
                     /old_locus_tag="pgaptmp_002353"
     CDS             complement(6188..7303)
                     /gene="yliI"
                     /locus_tag="ACHKNP_RS11765"
                     /old_locus_tag="pgaptmp_002353"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HGU4704507.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(7414..7797)
                     /gene="bssR"
                     /locus_tag="ACHKNP_RS11770"
                     /old_locus_tag="pgaptmp_002354"
     CDS             complement(7414..7797)
                     /gene="bssR"
                     /locus_tag="ACHKNP_RS11770"
                     /old_locus_tag="pgaptmp_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HGU4704508.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            8010..9335
                     /gene="rimO"
                     /locus_tag="ACHKNP_RS11775"
                     /old_locus_tag="pgaptmp_002355"
     CDS             8010..9335
                     /gene="rimO"
                     /locus_tag="ACHKNP_RS11775"
                     /old_locus_tag="pgaptmp_002355"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HGU4704509.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(9382..10710)
                     /gene="dgcI"
                     /locus_tag="ACHKNP_RS11780"
                     /old_locus_tag="pgaptmp_002356"
     CDS             complement(9382..10710)
                     /gene="dgcI"
                     /locus_tag="ACHKNP_RS11780"
                     /old_locus_tag="pgaptmp_002356"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU4704510.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYYAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(10718..13066)
                     /gene="pdeI"
                     /locus_tag="ACHKNP_RS11785"
                     /old_locus_tag="pgaptmp_002357"
     CDS             complement(10718..13066)
                     /gene="pdeI"
                     /locus_tag="ACHKNP_RS11785"
                     /old_locus_tag="pgaptmp_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4704511.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVLERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(13244..14155)
                     /gene="gsiD"
                     /locus_tag="ACHKNP_RS11790"
                     /old_locus_tag="pgaptmp_002358"
     CDS             complement(13244..14155)
                     /gene="gsiD"
                     /locus_tag="ACHKNP_RS11790"
                     /old_locus_tag="pgaptmp_002358"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HGU4704512.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(14158..15078)
                     /gene="gsiC"
                     /locus_tag="ACHKNP_RS11795"
                     /old_locus_tag="pgaptmp_002359"
     CDS             complement(14158..15078)
                     /gene="gsiC"
                     /locus_tag="ACHKNP_RS11795"
                     /old_locus_tag="pgaptmp_002359"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HGU4704513.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(15096..16634)
                     /gene="gsiB"
                     /locus_tag="ACHKNP_RS11800"
                     /old_locus_tag="pgaptmp_002360"
     CDS             complement(15096..16634)
                     /gene="gsiB"
                     /locus_tag="ACHKNP_RS11800"
                     /old_locus_tag="pgaptmp_002360"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HGU4704514.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYIVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(16654..18525)
                     /gene="gsiA"
                     /locus_tag="ACHKNP_RS11805"
                     /old_locus_tag="pgaptmp_002361"
     CDS             complement(16654..18525)
                     /gene="gsiA"
                     /locus_tag="ACHKNP_RS11805"
                     /old_locus_tag="pgaptmp_002361"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HGU4704515.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(18512..19477)
                     /gene="iaaA"
                     /locus_tag="ACHKNP_RS11810"
                     /old_locus_tag="pgaptmp_002362"
     CDS             complement(18512..19477)
                     /gene="iaaA"
                     /locus_tag="ACHKNP_RS11810"
                     /old_locus_tag="pgaptmp_002362"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU4704516.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            19681..20916
                     /gene="moeA"
                     /locus_tag="ACHKNP_RS11815"
                     /old_locus_tag="pgaptmp_002363"
     CDS             19681..20916
                     /gene="moeA"
                     /locus_tag="ACHKNP_RS11815"
                     /old_locus_tag="pgaptmp_002363"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HGU4704517.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRHGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            20916..21665
                     /gene="moeB"
                     /locus_tag="ACHKNP_RS11820"
                     /old_locus_tag="pgaptmp_002364"
     CDS             20916..21665
                     /gene="moeB"
                     /locus_tag="ACHKNP_RS11820"
                     /old_locus_tag="pgaptmp_002364"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HGU4704518.1"
                     /translation="MTELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(21741..22403)
                     /gene="fsa"
                     /locus_tag="ACHKNP_RS11825"
                     /old_locus_tag="pgaptmp_002365"
     CDS             complement(21741..22403)
                     /gene="fsa"
                     /locus_tag="ACHKNP_RS11825"
                     /old_locus_tag="pgaptmp_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU4704519.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGNKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            22534..23433
                     /gene="ybiY"
                     /locus_tag="ACHKNP_RS11830"
                     /old_locus_tag="pgaptmp_002366"
     CDS             22534..23433
                     /gene="ybiY"
                     /locus_tag="ACHKNP_RS11830"
                     /old_locus_tag="pgaptmp_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGU4704520.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            23439..25871
                     /gene="ybiW"
                     /locus_tag="ACHKNP_RS11835"
                     /old_locus_tag="pgaptmp_002367"
     CDS             23439..25871
                     /gene="ybiW"
                     /locus_tag="ACHKNP_RS11835"
                     /old_locus_tag="pgaptmp_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HGU4704521.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            26017..26832
                     /gene="ybiV"
                     /locus_tag="ACHKNP_RS11840"
                     /old_locus_tag="pgaptmp_002368"
     CDS             26017..26832
                     /gene="ybiV"
                     /locus_tag="ACHKNP_RS11840"
                     /old_locus_tag="pgaptmp_002368"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HGU4704522.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            26984..28249
                     /gene="ybiU"
                     /locus_tag="ACHKNP_RS11845"
                     /old_locus_tag="pgaptmp_002369"
     CDS             26984..28249
                     /gene="ybiU"
                     /locus_tag="ACHKNP_RS11845"
                     /old_locus_tag="pgaptmp_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HGU4704523.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVISPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         7155 a         7520 c         6903 g         6814 t
ORIGIN      
        1 tatcagcaaa tgaaagcaca aggcattcgc tttgtggtcg ccagcgggaa tcaatattat
       61 cagttgatct ctttcttccc tgaaattgcg aatgaaattg cctttgtggc tgaaaacggc
      121 ggctgggtag tgagtgaagg caaagatgtt tttaatggcg agctgtcgaa ggatgcgttt
      181 gctactgtcg tggaacattt gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa
      241 aatagcgcct atacactcaa aaaatatgac gatgccatga aaacggtggc ggaaatgtat
      301 tatcaccgtc tggaatacgt cgataacttt gacaacttag aagatatttt ctttaagttt
      361 ggcctgaatc tttccgatga actgattcca caagtacaaa aagcattaca tgaggccatc
      421 ggcgatatta tggtgccagt ccacaccggc aacggcagca tcgatctgat tatccccggc
      481 gtacataaag ccaatggcct tcgccaactg cagaaattat ggggaataga cgacagcgaa
      541 gtggtggtct ttggcgatgg cggtaacgat attgagatgc tgcgtcaggc aggctttagt
      601 tttgcaatgg aaaatgccgg cagcgcggtc gtcgcagcgg caaaataccg cgcaggctcc
      661 aataaccgtg aaggcgtact ggatgtgatc gataaagttc ttaaacacga agcgccattt
      721 gaccaataaa aacggcagca cgcccggcaa cacaatgcct gaatgaaggc tgcataattc
      781 gcggaaatta acccgctgca ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt
      841 gggcgcgctt ttaatgggct ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt
      901 gccgagtgga tacaaagtgg ttgtggttcc tgattcagtc agcgttccac aagcggtcag
      961 cgtcgcgact gtgccgcaaa ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc
     1021 gcatccttat gttcgccatc ttgccagcgt aggtgaaggg atggttatcg aacaccagat
     1081 tgacgaccat caccattaat tcgagaatgc ctgatcgcac aaagcaatca ggcatttttt
     1141 tacccttcgt gagaatttcc catctgttta tcttttaaaa agataaccat cagcgacagc
     1201 cacaaaattc cgttgacaag gttgaagaga ttaaacagtc cgttgccccc gttcagccag
     1261 gcatgtttgc tgatttcaat accgacggta aagatcagca tttgcagcat tcccatcgcc
     1321 gcagaaaccg tacctttact catatcgctg gcaaacaggg ttaatcgcac cagtcccgca
     1381 ttcgccagac caatgccgaa agcataaata cttaacccgg cggtcatcca taaatacgcg
     1441 tgcgatgaga taaccgttgc cgcagcagcg accaacagac caatcataat cggccagccg
     1501 cccataatta tcagcgaacg tacggtgcgg cgcgaggtca aacgcgctaa caacaagtta
     1561 cccgcaatta acgccccgaa aataggcact tgcagcaagc catattcata gctgctcaac
     1621 tgctcgccgg taatgatgat aatcggcgac tgggcgatcc acgccagcaa tggcaggcta
     1681 acgaatccca gcgccagcgc ccccgccaca aagcggccgt tcttcagcac cagcttatag
     1741 tcacgaccga gttctttcag tgacagtttc tcgcctatac gcgtggcggt ttcaggcatg
     1801 gcttgttgca gaccgaaaaa ggagatcgct gccaatgcgg caaacaagac aaacatccct
     1861 tcccagggca gcacatggat ccacgccgcg cccaccagcg gaccaagtag cggagcaatc
     1921 agcgccacgt tcgccatcag cgcggtgatc ttgatacaaa ccgcctcttc gaaggattcc
     1981 tgaattgcgg cgtatcccac agcgccaatg aaacagaggc ttatgccctg caagaagcgc
     2041 aacagagtga attgttcaat gttttgcgcc agtaatattg ccagacaggt gatgataaac
     2101 cacaccaccc ccgccagcat caccggacgg cgaccaatac gatccgacag cggccccagg
     2161 agccattgta aaaacatccc gcccgccaga tatgcggtca ttgaagtagg aacccaatca
     2221 atgcccgcct gatattgttc caccacggcc aacataccgg gttgaatcat atcgttgccg
     2281 atataggttg aaaattcgta aagcaccaga cagagaggga aaagtaacgc ctgacgtcca
     2341 agcctggcac cggaagctaa tttattttgc atgcaatttc ttcgccaata ataatcgcgc
     2401 agagtttaat aaaagcacag ctaacgagaa agcgaatttt gtagctgaaa ccacggttaa
     2461 gcacattctt acattattgc gagtatagct acgctttctt taagttttat ttaacctatg
     2521 cccgttacaa ttacccaccg taaacaggcc gcttgaggga aataagacga tgccgcttta
     2581 cccagtttaa cctgcacttt attctcaacg acttgcctgt attggctccc ttttaatcac
     2641 tttgcgtcgg gaagttatgc tggaaaattt gaatctctct ctattctctc ttattaacgc
     2701 gacgccagac tcggctccgt ggatgatctc gttggcgatt tttattgcta aagatttgat
     2761 taccgtggta ccgctgctgg ccgcggtgct ttggttgtgg gggcttacag cacaacggca
     2821 actggtgata aaaatcgcta tcgcgctggc ggtcagcctg tttgtgtcct ggacgatggg
     2881 acatcttttt ccgcacgacc gaccctttgt cgaaaatatc ggctataact tcctgcatca
     2941 tgcggcggat gactcattcc caagcgacca cggtacggtg attttcacct ttgcactggc
     3001 atttttatgc tggcatcgcc tgtggtccgg ctcactttta atggtgctgg ccgtcgtcat
     3061 tgcctggtcg cgcgtttatc ttggcgtgca ctggccgctg gatatgctcg gtggattgct
     3121 ggctggtatg attggctgcc ttagcgctca gattatctgg caagcgatgg ggcataaact
     3181 ctatcaacgt ctgcaatcgt ggtatcgctt ctgttttgca ttaccgatcc gcaaaggctg
     3241 ggtgcgtgac tgatctctgc taaaaagtgt agtattgagc ggctcgcttc aataactatt
     3301 cagagggatt atggaaacac gtcgcgaaga gcgcatcggg cagctgctgc aagaattaaa
     3361 acgcagcgat aagttacatc ttaaagacgc cgccgccctg cttggggttt cggagatgac
     3421 gattcgtcgc gatctgaaca accacagtgc gcccgtcgtt ttgctcggcg gctatattgt
     3481 tctggaaccg cgcagtgcca gccattacct gttaagcgat caaaaatccc gcctggtgga
     3541 agaaaaacgc cgggcggcaa aaatggctgc gacactggta gaacccgatc agactctctt
     3601 ttttgactgt ggcaccacca cgccgtggat tattgaagcg attgataatg aaatcccttt
     3661 taccgccgtt tgttattcgc taaatacctt tctggcgctg aaagagaaac cccattgccg
     3721 cgcatttctg tgcggtggtg aatttcacgc cagcaacgcc attttcaaac ccatcgattt
     3781 tcagcaaacg ttgaataatt tttgcccgga tatcgctttt tattccgcgg cgggcgtgca
     3841 tgtcagtaaa ggcgctacct gttttaatct tgaagagttg ccggtaaaac actgggccat
     3901 gtcgatggcg caaaagcatg tgctggttgt cgaccacagt aaatttggca aggtgcgtcc
     3961 ggcgcgcatg ggtgacctga aacgctttga tattgtggtg agtgattgtt gcccggaaga
     4021 tgagtatgtg aagtacgcgc agacgcaccg tattaagttg atgtattaat gacgtataac
     4081 cggatgacgt ttcgcgccat ccggttatca gaagattaag agaaccagct gccgaaccac
     4141 tgatggaatt tcatcatcac gaaatcccac atccgaccaa agaatccgcc ctcttccaca
     4201 ttttccatca cgatcagcgg acgctgctca atggatttac cgttaagctg gaaatcaatg
     4261 gtcccgacaa cctgaccttt tttcagcggt gcggtaagct gcggttccgt taacgtgtag
     4321 ctcgccttca ggtttttcag ctgcccacgc ggtatggtca ctgagcccgc ttcgcctgcc
     4381 ccaagattca cttcgctctt atcaccaaac cagacgcgct gagtcacaaa ggtggcatca
     4441 ggtttaattg gcgtcacggt ttcaaagaag cggaaacccc aggtcaataa tttctcagac
     4501 tcattaaaac ggatacggtc agttttcgcc cccagcacta cggagattaa acgcatatcg
     4561 ccctgggtag ccgaagcaac cagattatat cccgcgcctg ccgtggttcc tgtcttcatg
     4621 ccatcaacat tcagattgct gctccacagc agacggttac ggttaggctg acgaattttg
     4681 ttgaaggtga actctttctc tttatgaatg gcgtactctt ccggcacatc gtggatcaat
     4741 gctttaccca gcaatgccat atcgcgcgcg gtactgaact gccccggcgc atccagaccg
     4801 tgcaccgtct ggaaggtagt gttggtcaga cccagttttt tggcataacc attcatcaaa
     4861 ccaatgaatg actcctggct cccggcaacg taatcggcca gcgcaataca ggcgtcatta
     4921 ccggactgga taatcacacc tttgttcaag tctgccaccg aaacctgatc gcccggtttg
     4981 aggaacatta ccgatgaacc acgcagtgcc ggatttcccg tcgcccaggc atctttaccg
     5041 accgtcacca tatcggtgag tttaatctta tcggccttaa gcgcctgccc aaccacatag
     5101 ctggtcatga ttttagtcag gctcgcggga tccagtttct catccgcgtt gccttctgcc
     5161 agcactttac cgctggcgta atccattaaa atccatgcac gcgcatccac gctcggcgct
     5221 tcaacggttt gttccgccgc gaatgccgtt ggggcaaaaa ggaataaaaa tgcagaaccc
     5281 gctgcaagac cacgaaggag agaggagtat tgcgtcataa atgccacccg agtatccatt
     5341 caaaaaaaat acgttatatc gccgtatgat aagttcggtg attaataacg cacacacaaa
     5401 cttaaagaaa ccgtaagttg gtaaagtttt taaagtttat gcaatatcct gccgcttcgc
     5461 tgcttctggt gtcatttttt tgccagctgt tgcttaaaag ttaactttac ttcaccatcg
     5521 gagtcctgtt cagcctgtgg ctgcctgtcg gaaaaggggt gagtatgatt acgctgtggg
     5581 gtcggaataa ttcaaccaac gtaaaaaaag tattgctgac gctcgaagaa ctggaactac
     5641 cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct gattttctgg
     5701 cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat ctcattcttt
     5761 gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa cgtctgtgga
     5821 tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca aaccagacgc
     5881 tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg gaagagcgcg
     5941 atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc ctgctcgatg
     6001 cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc gatatcgcta
     6061 tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg cgtccaaatc
     6121 tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg gtgatgattc
     6181 ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg tcggtgagaa
     6241 cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct ctgtccgtta
     6301 aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg acatctttat
     6361 ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg ctgttataga
     6421 aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc tcggtcccgg
     6481 cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt ccccaggttg
     6541 ccagcggcca gccgtagttt ttgccttttt gcgggatatt aatttcatca ccaccgcgcg
     6601 ggccatgttc attcagccac agtgcattac tccacggatt cattgccatt ccttgcggat
     6661 tacgaatgcc ataagaccag atctcggcgc gcacaccgga ttcctttata aaaggattat
     6721 catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat ttatccagat
     6781 cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa cctttaccgt
     6841 cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc tggcggaaga
     6901 cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa cccacagcag
     6961 ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg cgagactgag
     7021 caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc caaacgtccg
     7081 gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc aactcgccgc
     7141 cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc cagggatggt
     7201 cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag agagcggaag
     7261 aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct ccggattgac
     7321 tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa aaacttaaac
     7381 atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca tggcgtgctt
     7441 ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct tatcggcgtt
     7501 gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg cggctgtcgc
     7561 taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat gaagattcaa
     7621 ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc gtagatgatt
     7681 gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg cagcgaggtc
     7741 aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa cgaacatact
     7801 tcgatcctcc tctttccagc cttgttgcaa ttaaatagta tggctatttt ttgagcaaaa
     7861 tgctgtccag gatcaattct tcacgccgaa ttaacaaaat ttacatttcc ttcgcctccg
     7921 ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc ctgccctgac
     7981 ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag ccgaaaatcg
     8041 gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt ctcaccgaac
     8101 tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg gtgatcgtca
     8161 acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt ggtgaagcgt
     8221 tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa gatcagatcc
     8281 gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag caggttctgg
     8341 agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc ctggtgccag
     8401 aacaaggtgt gaaactgacg ccgcgtcatt atgcctatct gaaaatttcg gaaggctgta
     8461 atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg agccgtccga
     8521 ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa gagattctgg
     8581 tgatctcgca ggatacttct gcctatggcg ttgatgttaa acatcgtact ggcttccaca
     8641 acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg aaactgggga
     8701 tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc atcccactga
     8761 tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc agcccgcgca
     8821 ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc atcaaacagt
     8881 ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc ttccctggcg
     8941 agacagaaga agatttccag atgctactcg acttcctgaa agaagcgcgt ctggatcgcg
     9001 ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg cctgaccagg
     9061 ttccggaaga agttaaagaa gaacgctgga accgtttcat gcagctgcag cagcagattt
     9121 ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc gacgaagtgg
     9181 acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac ggcgcggttt
     9241 atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa gtcgagcacg
     9301 ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg caaaatcatt
     9361 tgtccggcct gcgaataatg attactgcca gctaatctga gccctgccgt ttttcttact
     9421 cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac attcatttga
     9481 atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat gaacgatttc
     9541 ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg caaattcgtc
     9601 accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa ggattttcac
     9661 cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa tgtttttgaa
     9721 tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt ttaaatcttc
     9781 gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg agttatacat
     9841 actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca gtacgcgttc
     9901 aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca ctctgcgtac
     9961 cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga aaataatcaa
    10021 tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc cggtcggcaa
    10081 ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag cataatattc
    10141 atcaccggtc accggtttac cgttcttaaa tagagagaac atcaggttct cgctgtccat
    10201 cgccagcgat tttgacagta aagaaaaacg ctccagacca agcaaaaata tcagactacg
    10261 attgtgagtt accggataga ggtaatagac gaaggtttta ccgtcaaaag agataaaccc
    10321 tgtatttttg acatgtcgat acttcacaac ctgcataata tctgcacaat aatttttctc
    10381 gaactccttt acctgaatac attcgtcact ggaaatcaga taaggaccga catcgtacaa
    10441 ataagagggg gaaaccgagt cggcgtaagc tgtgcgcaaa ccaaccaaca gctgttcctc
    10501 cgtgggattt agcgtgttga taagatcaat tgccacgcta cgccgatatt cagcaatatt
    10561 tgccaccgca aaagcagtat caataacgga ctgtttcagg ctatacaccc gttcctttac
    10621 catttgagtg tagatcccgc aggcaactgc tgaaatcatg ataaaaatca gcagactgac
    10681 tgtaagtacg aacttattga ttctggacat acgtaaacta ctcttttact aattttccac
    10741 ttttatccca ggcggagaat ggcatgggtt tctggtacag atacccctga gcctgaacac
    10801 atcctaattt aattaattcg cgcgcaacgt cttcgctctc tacgccttcc gcaatcaccg
    10861 ggatccggtt atagcgtgcc agatcgaaaa taatgcgcac aatttttccc gcagtaccga
    10921 aaatatcgcc ggtaaattgc gagtccacct ttatgctgtc tggattaatt tcataaaaac
    10981 gtttcaggtt tgacaaaccg gtgccaaagt catcaatcga tatccgtacc ccttgtttac
    11041 gcagttgttc aatattgaga taaaaatgtt cattaattcg ctcgatcacg gtttcagtga
    11101 tctcaacaca gaggctgttc gccttcaggc cattttcttt catgatggtg gtaaaacgct
    11161 catgaaagtg cggttcgttt aactgccagg gcgaaacatt aatatgcagt aagaaatcat
    11221 ccttcagcga cgcacgctga ctaaaatgtc tgaattcacc cattgccgtt ttaataatct
    11281 gataacccag ctcattgata aacccgatat cttcagcaat ggtaatgaac ttcagcggtg
    11341 aaatgatccc ccgctccgca gaaacccaac ggcacaacgc ctccgcgccc agaatggcgc
    11401 gggtgttaat atcaacgatc ggttgataga cgaggaatag ttcgccaatt tccattgcat
    11461 ggcgaatatt acgcgcaatc cagatattct cttctatttc gttgagtttc gcgtcatcac
    11521 agaaaataaa ctcggagaac atatggtcac ggatactgga aatcgccagt ttcagttttt
    11581 ctgcgtaact ccaggcgttt tccccctgca aaggttcaca aatggcagac ttatggatgc
    11641 gcagattgcg gtagccaaag ctgctgtcaa aatcgtttac catcgctatt ttacgggtaa
    11701 atgtctgtac attttcccga cacagaagta aatagagatc ggcattgaat gtcacgatat
    11761 cgcagcattg cgcatatttc tcggcaattc gctgacacaa tttcctgacg attttattgc
    11821 gttcatcaac accaaacacc gcttccacgg cttccagacc acacagtttg atgatcccca
    11881 cagccagata ctgattattg ttggactcaa taatcgccat atttttaata aaaccgcttt
    11941 cctgatcgta agagatcagt gagagcaatt tatttgaccg ttcaatttcg gcatctttca
    12001 tttccgcgat cgaatttttg atgctgataa tctctttcgc atcgccttcg cgaattttca
    12061 gagcctcgat ctgatccgtt gagtcgggca aaaacgccag cgtattaacc agctcggtca
    12121 tttcagaaaa ttgccgacgg aacacacgag tggtcatgaa atataccacc agcaacacga
    12181 taataatgaa cgcaaacggt agataaaaaa gtttatctga tgcgttcaat aaattaccgc
    12241 caggataata aaaatagatt tcgaacaatg cattatttgg cactgctgat gtctttgcaa
    12301 tatactccgt atcgacagaa tcaataatat tataattacc ttcgctatta taaataatca
    12361 gtttttcacg agcgagttga ttcttggcaa tgatctcatg accttgtttc aaaaatagaa
    12421 cttcatcatt agcgctgatt tgatcaagat actcgttcag ataccgccag tttaaatcga
    12481 catgaatttt atctaccgct aacaaatcgt gatctttgcc gttgatacgt agcgtaaaag
    12541 agatacattt tttacccgga cgatcaaagc aattgtatgc cggattccag ctgttgttag
    12601 agtcatctga agccaccgac cacggtcgtt tcgatgggtt aaaatcggca aaaataaccc
    12661 gccctgactc gtcgacaagc ggtccattta cttgttcctg atgataaaca acgattttat
    12721 cattcgccct ccgtgaaaag agataatatt tattatcatc aagtaccagt cccaccgagt
    12781 ctaaaaatgg catcatttcc attttcagaa tcacggcgcg ttgaatctcc tctgtatttt
    12841 ttaataaata cagtgagtcc tttaatgtca gggccgaacg ttcaatatct ttaaagaaat
    12901 agccaacgtt tttctgaata agttcaaagc ggctatcggc acgttttacc gcgcgttcca
    12961 ataccagttg atagttaatg atgaaaaaaa gaccaataaa tgacagtgct gccaataaaa
    13021 ataaaattat cagccttatc tttatctttt cgtataaact cagcatgtta atacatttaa
    13081 caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc ggctttttaa
    13141 ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta gggcaataat
    13201 ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct ttaattttcg
    13261 gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg gtcagaaaaa
    13321 tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct cgtgcctcat
    13381 tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga aatgacaggc
    13441 tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg atagacgaga
    13501 cggtcccagg caagatatga cgcaataaaa tggtcatatc gctggcacca atactgcgtg
    13561 ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc agtcgggcaa
    13621 acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg cttcccaaca
    13681 ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca tcgcaaatgc
    13741 gcatgatcaa ccgatcccac cagccttcat aatatccagc caacaagccc agcaacgtcc
    13801 cgatcgccgc accgataaac acggcaaaca cgcccgccgc cagcgagatt tgcgcaccaa
    13861 ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag tgctgcaaag
    13921 aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag ggagcgatcc
    13981 agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg gtcatcgcca
    14041 tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgcacc tggtcaggtt
    14101 tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt cgcatcctta
    14161 cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta agttgataag
    14221 aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat cgcgcatttc
    14281 tacggagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct ccaccacgat
    14341 agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg ggatcatcgc
    14401 gttacgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg ccctcgcggt
    14461 acgcatataa tcttcgctta aaacatcgac aaacgacgcg cgggtaaagc gcgccatcac
    14521 ggctgccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc aactgtctgc
    14581 tcccacggta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa gcatccccag
    14641 cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc gatccggcca
    14701 acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca ccgcccagac
    14761 catactggtt atggtcagcc acagcgttgg cataaagcgg ctggcaatct catcggcaac
    14821 cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc tgatatagtg
    14881 ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca gttctataac
    14941 ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca gcatatgcac
    15001 aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca gtaagcgttt
    15061 gataacgtaa ttaagcattc cactccctgc gttgcttatt gcaaatccgc gtcttcaaag
    15121 ctgaagccgg tgtctggcat gatccaaaaa ccggtcaggt ttttactgtg tgccgacacc
    15181 agtttttcta ccaccagcgg gatccacggc gactcttgcc agatgatatc ctgcgccgcc
    15241 ttatacaagc gggtcttttc cgccggatca ttagttttca gtgcctgagc caggaagtcg
    15301 tccacctgtt tattgctgta aaatgcggta ttaaacagcg tcggtggcca gttttgcgag
    15361 gcgaacagtg gcgatagcgc ccagtccgct tcgccggttg aagccgacca gccagtgtag
    15421 aacatccgca cgccgctctc tttttgccct ttaccttcaa cttctgccgc ccgctgtccg
    15481 gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg ctgggtaaat
    15541 tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt actgaaaccg
    15601 ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta aggccacggt
    15661 ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg cgttgcatag
    15721 cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag cgcctcacgg
    15781 accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg ctgcataatt
    15841 gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc ctgctcgtag
    15901 ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg ggtgttgtta
    15961 tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg ccagtaaccc
    16021 gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc atacggtccg
    16081 gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgc cggtgaaatc
    16141 atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg tttgagggta
    16201 atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata caggttatag
    16261 cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac cgccactgcg
    16321 ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacgat gtaagtaatg
    16381 ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat ctctttatcc
    16441 agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt gtcatttgcg
    16501 tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc tttggcagcg
    16561 aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc actacggtgt
    16621 acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg cgtattctga
    16681 ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg ctgccacctc
    16741 ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac gctgcggtcg
    16801 ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat aaggatgctg
    16861 cgggttttcg aagaccgcgc gccgtggacc aatttcaaca atttgcccga gatacatcac
    16921 cgccacacga tgactaatcc gctctaccac ggccatatcg tgggagataa acagatacgc
    16981 aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc gaatagaaac
    17041 atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg ccaacgcgcg
    17101 agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac gccaggcatg
    17161 ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg cgtctttacc
    17221 tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg tctgacgtgg
    17281 gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca atgcctgaag
    17341 tttgccgggt gacaaggtat cgattctctg accgttaaag ataatttcgc cgccctgcga
    17401 ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag actcgcccac
    17461 cagcgatagc gtttcgccag gccagagatc aaaactaact ttctcaacgg catgcacttc
    17521 ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa gattacgcac
    17581 tcgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atgggggggg cctgtttcgc
    17641 tggatgttca agcgatatca acgggaaacg tcggggataa tctaaccctt tcatcgcacc
    17701 aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg catgaaaaat
    17761 ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta cccgatcggc
    17821 aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg acatctcttt
    17881 ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca gcgcggtggt
    17941 tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa tcatcactcg
    18001 ctggcgcatc ccgccagaga gttgatgcgg ataacgtgaa agaatggttt gtgcctcagg
    18061 aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac gactggcgtt
    18121 ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaataccg ggttcagcga
    18181 tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgaacatggc gcatttgtgc
    18241 agcgctctgc tcgctaagtt caatcacttc gcgactgcgc cgctgcaaca gcattttatc
    18301 gcactgtact aaaccgcccg cctgttccaa caggcgcatc aatgccaacg cagtcactga
    18361 cttaccggag ccggattcgc caacaattgc cagcgtctca ccgcgttgca gactaaaaga
    18421 gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat tcagattttc
    18481 aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg tccccttttt
    18541 cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga tacattcctt
    18601 cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag ccaccgctac
    18661 cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg agacttaatc
    18721 cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg aagacttcgc
    18781 ccgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg gcacccacta
    18841 agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg gacgtggctg
    18901 ccgccaaatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt ttctgttttt
    18961 catccagtgg cgcaccgcta tggtcgagga cggttgcccc ttccttgcgc gctgccagta
    19021 gttgttcata acgcaaaggc gtggagaaaa tctccggcga gacgcgctcc atgccacgag
    19081 caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt tgctccatca
    19141 ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc gcaccggctt
    19201 tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca cgcgtaaaga
    19261 cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc gcttccgtca
    19321 ccacatccag cgcactttcg cccgcttcca acattttctg cccggtttca acaatggcag
    19381 acaacgcctc aatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg ctaattgcac
    19441 ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat taagggtggc
    19501 tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat ttttgaatat
    19561 agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg tggcatacag
    19621 tctgtggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag gagtgttttc
    19681 atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat gctttctcgc
    19741 gtcaccccac tgaccgccca ggaaacgctg ccactggtac agtgttttgg tcgtattctg
    19801 gcgagcgatg tcgtttcgcc gcttgatgtt ccggggtttg ataactccgc aatggacggc
    19861 tacgcggtgc gtttagccga tattgcctcc aggcaaccgc tgcccgttgc cggtaaatcc
    19921 tttgccggtc agccgtatca tggtgaatgg cctgcgggca cctgcattcg tattatgacc
    19981 ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac tgaacaaacg
    20041 gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg ccgtcacggt
    20101 gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac taccgcagag
    20161 ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa agtgcgtgta
    20221 gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg cgacggccaa
    20281 atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg ttgcgaggta
    20341 atcaacttag ggattatccg cgacgatccc catgccctgc gcgccgcatt tattgaagcc
    20401 gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga agcggattac
    20461 accaaaacga ttcttgaaga gctgggggag atcgccttct ggaagctggc gattaaacca
    20521 ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct gccgggcaac
    20581 ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc aaaactaagc
    20641 ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc ccgcctgaaa
    20701 aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc cgacggtgaa
    20761 cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt tagtctcggc
    20821 aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg aagtgggcga atgggtggaa
    20881 gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatgac ggaactcagc gatcaggaga
    20941 tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt caggaggcgc
    21001 taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca gcttcgcagt
    21061 atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgactcg gtttcgctct
    21121 cgaatctgca acgccagacg ctgcacagtg atgctacggt cgggcaaccg aaggtagaat
    21181 ccgcccgtga cgccatgacg cggatcaacc cacatatcgc gattacgcca gtcaatgcac
    21241 tgcttgatga cgctgaactt gctgcgatga ttgcaaaaca cgatctggta ctcgactgta
    21301 cggataacgt tgcggtgcgt aatcaactga acgctggctg ttttgccgcg aaggtgccgc
    21361 tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact tatcaggacg
    21421 gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcatta acctgcgtag
    21481 aagcaggcgt gatggcaccg ttgatcggcg taattggttc gttgcaggca atggaagcga
    21541 tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg tacgatgcaa
    21601 tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag gtgtgcgggc
    21661 agtaattgcc acctccaggc cagataagac gcggcactcc gccacgtcat cagcaattta
    21721 tgaggatgca gaacgtccgg ttaaatcgac gttctgccaa acgctcccag ccagtcctgt
    21781 tcaaacttcg ccacagcggc ttccaccgcc ggatagctaa tcatctgttg tgccacatcc
    21841 agtggcagcg taattgattc acatcctgcc agtaagcagt ccagcgcctg acgcggggtt
    21901 ttgaaactcg ctgccagcac tttcgcctgc ggcgcatgca ttttcaataa ctggtgtaag
    21961 tcggtcacag tctgaatgcc gctaccaccc tgagcatcaa tacgattaac gtaaggcgca
    22021 acatattccg cacctgccag cgccgacagc agcccttgtg ctgcgccata taccgcggtt
    22081 cccagcgttg gaatcccttc cgcttttaac atcttaatag ctgccagccc ctcggcggtc
    22141 accggaactt tcaccacgat atccgcaata atagaacgca atttacgtgc gtcattcacc
    22201 atcccttcag cagtggtagc cattacctgg gcaaacagac gcccctgacc gcccatcgct
    22261 tcatgaagtt gcggaagcac aacatccagc ggtttgttac ccgcggcgat aatgcttggg
    22321 ttagtggtca cacccgccag cggaaaaata cgtgacagcg ccttcaccgc aacaacgtct
    22381 gaagtatcca gatacagttc cataaccatc ctcaaaatgt actttttgtc tgaatgtaga
    22441 ctatcacggg aaaactctct gaacagttga cgcacatcaa tctaactttc attcgaaagc
    22501 attttaatgt ttgaacgaaa cagagaggca actatgattt tcaatattca gcgctactcg
    22561 acccatgatg gccccggtat ccgcacggtc gtatttctta aaggctgttc gctgggctgc
    22621 cgctggtgtc agaacccgga aagccgcgcc cgcacgcagg atctgctgta tgacgcacga
    22681 ctgtgtctgg aaggctgcga gctgtgcgct aaggccgcgc cggaagtgat tgagcgcgcg
    22741 ctgaatggtt tgcttattca tcgggaaaag ttaaccccgg agcatctgac ggcgttaacc
    22801 gactgctgtc cgacacaggc attaaccgtg tgtggtgaag tgaaaagcgt tgaggagatc
    22861 atgacgaccg ttctgcgcga taaaccgttt tatgatcgca gcggcggcgg tttaacgctt
    22921 tcgggtggtg agccctttat gcagccggaa atggcgatgg cgctactgca agccagccac
    22981 gaggcaggca ttcatactgc ggtagaaacc tgtctgcatg tgccgtggaa atatatcgcc
    23041 ccttctctgc cctatatcga tctgtttctt gccgatttaa aacacgttgc cgacgcgccg
    23101 tttaaacagt ggaccgacgg taacgccgcc agagtgctgg ataacctgaa aaaactcgcc
    23161 gcagcgggca aaaaaatcat tatccgcgtg ccgctgattc agggctttaa tgccgacgaa
    23221 acctctgtaa aagccattac cgattttgcc gccgacgagc tgcacgttgg cgaaattcat
    23281 tttctgccct accacacgct gggcatcaac aaatatcact tacttaatct gccctatgac
    23341 gccccggaaa aaccgcttga tgcgccagaa ctgctcgact ttgcccagca gtatgcctgc
    23401 cagaaagggt taaccgcgac cttacgagga taacaaccat gaccacactg aaactggaca
    23461 cgctcagcga ccgcattaaa gcgcacaaaa atgcgctggt gcatattgtg aaaccgccag
    23521 tctgtaccga gcgcgcgcag cactataccg agatgtatca acaacatctc gataagccga
    23581 tcccggtacg tcgcgcgctg gcactggcgc atcacctggc gaatcgcacc atctggatca
    23641 aacacgatga gttgatcatt ggcaaccagg caagcgaagt tcgcgccgcg ccgatcttcc
    23701 cggaatatac tgtctcgtgg atcgaaaaag agattgatga tctggcagat cgtcccggtg
    23761 ctggctttgc ggtgagcgaa gagaacaaac gcgttctgca tgaagtgtgc ccgtggtggc
    23821 gcggtcagac cgtacaggat cgctgctacg gcatgtttac cgatgagcaa aaaggtctgc
    23881 tggcgaccgg aatcattaaa gcggaaggca atatgacctc cggcgatgcg cacctggcgg
    23941 tgaatttccc gctgctgctg gaaaaaggtc ttgatggtct gcgtgagaaa gtagcggaac
    24001 gtcgctcgcg catcaatctg acggtgctgg aagatttaca tggcgaacaa ttcctgaaag
    24061 cgattgatat cgtgctggtg gcagtcagtg aacacattga acgtttcgct gccctggcgc
    24121 gtgaaatggc tgctaccgaa actcgcgaaa gccgtcgcga tgaactgctg acgatggcag
    24181 aaaactgcga tcttatcgcc caccagccgc cgcagacttt ctggcaggcg ctgcaactgt
    24241 gttacttcat ccaattgatt ttgcagatcg aatctaacgg tcactcggta tcgtttggtc
    24301 gtatggacca gtatctctac ccgtactatc gccgcgacgt tgaactcaac cagacgctgg
    24361 atcgcgaaca cgccatcgag atgctgcata gctgctggct gaaactgctg gaagtgaaca
    24421 agatccgctc cggctcacac tcaaaagcct ctgcgggaag tccgctgtat cagaacgtta
    24481 ccattggcgg acaaaatctg gttgatggtc aaccaatgga tgcagtgaat cccctctctt
    24541 acgcgatcct cgaatcctgc ggtcgcctgc gttccactca gcctaacctc agcgtgcgtt
    24601 accatgcagg aatgagtaat gatttcctcg acgcctgcgt acaggtgatc cgttgcggct
    24661 tcgggatgcc ggcgttcaac aacgacgaaa tcgtgatccc ggaatttatt aaactcggta
    24721 ttgaaccgca ggacgcttac gactacgcag cgattggttg tatcgaaacc gccgtcggtg
    24781 gcaaatgggg ctatcgctgt accggcatga gctttatcaa cttcgcccgc gtgatgctgg
    24841 cggcgctgga aggcgggcgt gatgccacca gcggcaaagt gttcctgcca caagaaaaag
    24901 cgttgtcggc aggcaacttt aacaatttcg atgaagtaat ggacgcgtgg gatacgcaaa
    24961 tccgttatta cacgcgtaaa tcaatcgaaa tcgaatatgt cgtcgacacc atgctggaag
    25021 agaacgtgca cgatattctc tgctcggcgc tggtggatga ctgtattgag cgagcgaaaa
    25081 gtatcaagca aggcggcgcg aaatatgact gggtttctgg cctgcaggtt ggcattgcca
    25141 acctcggcaa cagcctggcg gcagtgaaga aactggtatt tgaacagggc gcgattggtc
    25201 agcaacagct ggctgccgca ctggcggatg acttcgatgg cctgactcat gagcaactgc
    25261 gtcagcggct gattaacggt gcgccgaagt acggcaacga cgatgatact gtcgatacgc
    25321 tgctggctcg cgcttatcag acctatatcg acgaactgaa acagtaccat aatccgcgct
    25381 acggtcgtgg tccggttggc ggcaactatt acgcgggtac gtcatctatc tccgctaacg
    25441 taccgtttgg cgcgcaaact atggcaacgc cggacgggcg taaagcccac accccgctgg
    25501 cagaaggcgc tagtccggcc tctggtactg accatctcgg ccctactgcg gtcattggct
    25561 cggtgggtaa actgcctacg gcagcgattc tcggcggcgt gttgctcaac cagaaactga
    25621 atccggcaac gctggagaac gaatctgaca agcagaagct gatgatcctg ctgcgcacct
    25681 tcttcgaggt gcataaaggc tggcatattc agtacaacat cgtttcccgc gaaacgctgc
    25741 tggaggcgaa aaaacatccg gatcagtacc gcgatctggt agtgcgtgtc gcgggttatt
    25801 ccgccttctt caccgcgctc tctccagacg ctcaggacga tatcatcgct cgtactgaac
    25861 atatgctgta atctcttcgg cccggctgcg gcgtcgccgg gctaaatcat tcacatcatc
    25921 aatttcatcc ttactttcat tcgaaatata atttgtgctc tgcgtcacat tgttattaca
    25981 ctgttacggt ttcattttcg agccaggagc acacctatga gcgtaaaagt tatcgtcaca
    26041 gacatggacg gtacttttct taacgacgcc aaaacgtaca accaaccacg ttttatggcg
    26101 caatatcagg aactgaaaaa gcgcggcatt gagttcgttg tcgccagcgg taatcagtat
    26161 taccagctta tttcgttctt tcctgagcta aaggatgaga tctcttttgt cgcggaaaac
    26221 ggcgcactgg tttacgaaca tggcaagcaa ctgttccacg gcgaactgac ccgacatgaa
    26281 tcgcggattg ttattggcga gttgctaaaa gataagcaac tcaattttgt cgcctgcggt
    26341 ctgcaaagtg catatgtcag cgaaaatgcc cccgaagcat ttgtcgcact gatggcaaaa
    26401 cactaccatc gcctgaaagc tgtaaaagat tatcaggaga ttgacgacgt actgttcaag
    26461 ttttcgctca acctgccaga tgaacaaatc ccgttagtga tcgacaaact gcacatagcg
    26521 ctcgatggca ttatgaaacc cgtcaccagt ggttttggct ttatcgacct gattattccc
    26581 ggtctacata aagcaaacgg tatttcgcgg ttactgaaac gctgggatct gtcaccgcaa
    26641 aatgtggtag cgattggcga cagcggtaac gatgcggaga tgctgaaaat ggcgcgttat
    26701 tcctttgcga tgggcaatgc tgcggaaaac attaaacaaa tcgcccgtta cgctaccgat
    26761 gataataatc atgaaggcgc gctgaatgtg attcaggcgg tgctggataa cacatcccct
    26821 tttaacagct gacctctcta ccggagccgt gcgctccggt tttcccttct ttttacatcc
    26881 tgtacatcac atcaaatcat taaacttgca ttaacttatt tataacttat attgtcacat
    26941 gcaaatcaga atggtttcga atgaaagtta atgagaggtg gttatggctt ctacttttac
    27001 cagcgacaca ttgcctgccg atcacaaagc agctatccgt cagatgaagc acgcgctgcg
    27061 ggcgcagctt ggcgacgtcc agcagatctt taatcagcta agcgatgaca ttgccacgcg
    27121 agtggctgaa atcaacgcac tcaaagcaca gggcgatgcc gtctggccgg tgctgtctta
    27181 tgccgatatc aaagcaggtc atgttactgc agagcagcgc gaacagatta aacgtcgcgg
    27241 ttgtgcggtg ataaaaggcc atttcccccg cgaacaagcg ctaggctggg atcagtcgat
    27301 gctggactat ctggaccgca accgctttga cgaggtctac aaaggccccg gcgataattt
    27361 cttcgggacg ctcagcgctt cacgtcccga gatttacccc atctactggt cgcaggcgca
    27421 aatgcaggcc cgccagagtg aagaaatggc gaatgcgcag tcgtttctca atcgtctgtg
    27481 gacatttgaa agtgatggaa agcaatggtt taacccggat gtgagcgtca tctcccctga
    27541 ccgtatccgc cgccgtccgc ccggaacgac ctccaaaggt cttggagcgc ataccgactc
    27601 cggggcgctg gaacgctggc tgcttccagc gtatcagcgc gttttcgcca acgtctttaa
    27661 tggcaatctg gcgcaatatg atccctggca tgcggcacat cgtacggaag ttgaagagta
    27721 cacggtggac aacaccacca aatgttccgt gtttcggaca ttccagggct ggacagcgct
    27781 ctctgatatg ctgcctggtc aggggctgct gcacgttgtg cccattcctg aagccatggc
    27841 gtacgtactg ttacgtccgc tgcttgatga tgtgccggag gatgaactgt gcggcgtagc
    27901 gcccggaaga gtgttgccgg tatcagagca atggcatcca ctgttaattg aggcgttaac
    27961 cagcattcca aaactcgagg ccggagactc cgtctggtgg cactgcgacg tcatccattc
    28021 cgttgccccc gttgaaaatc aacagggttg gggcaacgtg atgtacattc ctgcggcacc
    28081 gatgtgcgag aaaaatcttg cctacgcgca caaggtgaag gccgcactgg aaaaaggcgc
    28141 atcgccgggc gacttcccgc gcgaggacta tgaaacaaac tgggaaggac gctttacgct
    28201 tgccgacctc aacattcacg gtaagcgagc gttgggcatg gatgtttgag agttaatgga
    28261 agtgaattga tgtgggatta agaaaatcga ttcgacgtta ctcagattgc tgacaagtgc
    28321 gctttcttaa tgcctgatgc gctacgctta tctggcctac aaagtcctgc agattcaata
    28381 tattacaggt ac
//
LOCUS       DAZFWL020000051        28314 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFWL020000051 DAZFWL020000000
VERSION     DAZFWL020000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.051"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(70..1173)
                     /gene="gldA"
                     /locus_tag="ACHKNP_RS11850"
                     /old_locus_tag="pgaptmp_002370"
     CDS             complement(70..1173)
                     /gene="gldA"
                     /locus_tag="ACHKNP_RS11850"
                     /old_locus_tag="pgaptmp_002370"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HGU4704524.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(1184..1846)
                     /gene="fsa"
                     /locus_tag="ACHKNP_RS11855"
                     /old_locus_tag="pgaptmp_002371"
     CDS             complement(1184..1846)
                     /gene="fsa"
                     /locus_tag="ACHKNP_RS11855"
                     /old_locus_tag="pgaptmp_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU4704525.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAISDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(1858..4359)
                     /gene="ptsP"
                     /locus_tag="ACHKNP_RS11860"
                     /old_locus_tag="pgaptmp_002372"
                     /pseudo
     CDS             complement(1858..4359)
                     /gene="ptsP"
                     /locus_tag="ACHKNP_RS11860"
                     /old_locus_tag="pgaptmp_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            4668..5747
                     /gene="frwC"
                     /locus_tag="ACHKNP_RS11865"
                     /old_locus_tag="pgaptmp_002373"
     CDS             4668..5747
                     /gene="frwC"
                     /locus_tag="ACHKNP_RS11865"
                     /old_locus_tag="pgaptmp_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HGU4704526.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            5762..6082
                     /gene="frwB"
                     /locus_tag="ACHKNP_RS11870"
                     /old_locus_tag="pgaptmp_002374"
     CDS             5762..6082
                     /gene="frwB"
                     /locus_tag="ACHKNP_RS11870"
                     /old_locus_tag="pgaptmp_002374"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU4704527.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            6133..8430
                     /gene="pflD"
                     /locus_tag="ACHKNP_RS11875"
                     /old_locus_tag="pgaptmp_002375"
     CDS             6133..8430
                     /gene="pflD"
                     /locus_tag="ACHKNP_RS11875"
                     /old_locus_tag="pgaptmp_002375"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU4704528.1"
                     /translation="MTNRISRLKTALFSNTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            8396..9274
                     /gene="pflC"
                     /locus_tag="ACHKNP_RS11880"
                     /old_locus_tag="pgaptmp_002376"
     CDS             8396..9274
                     /gene="pflC"
                     /locus_tag="ACHKNP_RS11880"
                     /old_locus_tag="pgaptmp_002376"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HGU4704529.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDVPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            9276..9617
                     /gene="frwD"
                     /locus_tag="ACHKNP_RS11885"
                     /old_locus_tag="pgaptmp_002377"
     CDS             9276..9617
                     /gene="frwD"
                     /locus_tag="ACHKNP_RS11885"
                     /old_locus_tag="pgaptmp_002377"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU4704530.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(9604..10455)
                     /gene="yijO"
                     /locus_tag="ACHKNP_RS11890"
                     /old_locus_tag="pgaptmp_002378"
     CDS             complement(9604..10455)
                     /gene="yijO"
                     /locus_tag="ACHKNP_RS11890"
                     /old_locus_tag="pgaptmp_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4704531.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10604..12337)
                     /gene="eptC"
                     /locus_tag="ACHKNP_RS11895"
                     /old_locus_tag="pgaptmp_002379"
     CDS             complement(10604..12337)
                     /gene="eptC"
                     /locus_tag="ACHKNP_RS11895"
                     /old_locus_tag="pgaptmp_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU4704532.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLHPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(12520..15171)
                     /gene="ppc"
                     /locus_tag="ACHKNP_RS11900"
                     /old_locus_tag="pgaptmp_002380"
     CDS             complement(12520..15171)
                     /gene="ppc"
                     /locus_tag="ACHKNP_RS11900"
                     /old_locus_tag="pgaptmp_002380"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGU4704533.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(15523..16674)
                     /gene="argE"
                     /locus_tag="ACHKNP_RS11905"
                     /old_locus_tag="pgaptmp_002381"
     CDS             complement(15523..16674)
                     /gene="argE"
                     /locus_tag="ACHKNP_RS11905"
                     /old_locus_tag="pgaptmp_002381"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HGU4704534.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            16828..17832
                     /gene="argC"
                     /locus_tag="ACHKNP_RS11910"
                     /old_locus_tag="pgaptmp_002382"
     CDS             16828..17832
                     /gene="argC"
                     /locus_tag="ACHKNP_RS11910"
                     /old_locus_tag="pgaptmp_002382"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HGU4704535.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPCCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            17840..18616
                     /gene="argB"
                     /locus_tag="ACHKNP_RS11915"
                     /old_locus_tag="pgaptmp_002383"
     CDS             17840..18616
                     /gene="argB"
                     /locus_tag="ACHKNP_RS11915"
                     /old_locus_tag="pgaptmp_002383"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HGU4704536.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            18677..20050
                     /gene="argH"
                     /locus_tag="ACHKNP_RS11920"
                     /old_locus_tag="pgaptmp_002384"
     CDS             18677..20050
                     /gene="argH"
                     /locus_tag="ACHKNP_RS11920"
                     /old_locus_tag="pgaptmp_002384"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGU4704537.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            20317..21234
                     /gene="oxyR"
                     /locus_tag="ACHKNP_RS11925"
                     /old_locus_tag="pgaptmp_002385"
     CDS             20317..21234
                     /gene="oxyR"
                     /locus_tag="ACHKNP_RS11925"
                     /old_locus_tag="pgaptmp_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HGU4704538.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(21217..22617)
                     /gene="sthA"
                     /locus_tag="ACHKNP_RS11930"
                     /old_locus_tag="pgaptmp_002386"
     CDS             complement(21217..22617)
                     /gene="sthA"
                     /locus_tag="ACHKNP_RS11930"
                     /old_locus_tag="pgaptmp_002386"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HGU4704539.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            22948..23598
                     /gene="fabR"
                     /locus_tag="ACHKNP_RS11935"
                     /old_locus_tag="pgaptmp_002387"
     CDS             22948..23598
                     /gene="fabR"
                     /locus_tag="ACHKNP_RS11935"
                     /old_locus_tag="pgaptmp_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HGU4704540.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFTSLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            23598..23957
                     /gene="yijD"
                     /locus_tag="ACHKNP_RS11940"
                     /old_locus_tag="pgaptmp_002388"
     CDS             23598..23957
                     /gene="yijD"
                     /locus_tag="ACHKNP_RS11940"
                     /old_locus_tag="pgaptmp_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HGU4704541.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(23997..25097)
                     /gene="trmA"
                     /locus_tag="ACHKNP_RS11945"
                     /old_locus_tag="pgaptmp_002389"
     CDS             complement(23997..25097)
                     /gene="trmA"
                     /locus_tag="ACHKNP_RS11945"
                     /old_locus_tag="pgaptmp_002389"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HGU4704542.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      25211..25401
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            25466..27310
                     /gene="btuB"
                     /locus_tag="ACHKNP_RS11950"
                     /old_locus_tag="pgaptmp_002390"
     CDS             25466..27310
                     /gene="btuB"
                     /locus_tag="ACHKNP_RS11950"
                     /old_locus_tag="pgaptmp_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HGU4704543.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            27255..28112
                     /gene="murI"
                     /locus_tag="ACHKNP_RS11955"
                     /old_locus_tag="pgaptmp_002391"
     CDS             27255..28112
                     /gene="murI"
                     /locus_tag="ACHKNP_RS11955"
                     /old_locus_tag="pgaptmp_002391"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGU4704544.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         6470 a         7121 c         7806 g         6917 t
ORIGIN      
        1 actgataagc gtagcgcatc aggctatttt gcgttcaaac tcccggacaa gccgggagtt
       61 tggagtaggt tattcccact cttgcaggaa acgctgaccg tactggtcgg ctaccagcag
      121 agcggcgtaa acctgatctg gcgtcgcgcc gccaggcatg ttgtggatgg tttcaccttc
      181 tgcacatgcc gcttctgcca caattcgcat tttcgccggg acatcttctt taatatccag
      241 ttgagcgaga gttattggca aacctaccgc atggctaagt gcagctacgg tttcgatttc
      301 ctcaaccggc gcgttttcca gtaccagctg cgtcagcgta ccgaatgcca ctttttcacc
      361 gtgataatag tgatgcgcgt ccgggatagc ggtcaggccg ttatgcaccg catgagccgc
      421 cgccagtccg ccgctttcaa aaccaacgcc gcttaaatag gtgttcgctt caatcacgcg
      481 ttccagcgca ggggttacta catgctgttc ggcggcaagc atcgcttttt cgccttcttc
      541 cagcagagtg ttgtagcaca gttctgccag tgccagcgcc gcctgggtgc acttgccgcc
      601 cgccatagtg gtagcgccgc tacgggaaca ggcgcgtgct tcaaaccagg ttgccagcgc
      661 atcgccgata cccgccgcta acagacgtgc aggagcgccc gcgacgattt tggtgtcgac
      721 aatgaccata ttcgggttat ttggcaacag tagatagcgg tcaaactcac cctcatcggt
      781 gtagataaca gacaatgcgc tgcacggtgc atcggtagag gcgatagtcg gtgcgatcgc
      841 taccggaaca cccatgaaat gtgccagtgc tttggcagta tcgagggttt ttccgccacc
      901 gataccgaga attgcgccac actgcgcagt ctccgcgatg ccacgcagac gatcgatctc
      961 attttgcgaa cattcaccgc caaacggcgc aatttctact accagtccag catctttaaa
     1021 gcttttctcg acagtggatt gagcaaaacc taaaacaaat ttgtcaccca ccactaacca
     1081 gcgttctgcc agcggcttca ggtattcgcc cagacgatta atcacatcag cgccctggat
     1141 gtatttaccc ggtgattgaa taatgcggtc cataattgct cctttagaga tgagtagtgc
     1201 caaatgcggc attccagtcg tgttcgaact tctctatagc tgactctacc gcaggggtgt
     1261 tgagcatttg ttgcgctaca tctaaaggca gggtgatgga ctcacatcct gccagcaaac
     1321 aatccagtgc ctgacgcggc gttttaaagc tggctgcaag caccatgctt tctggcgcgt
     1381 gcatttctaa cagcgcttgc agctcctgaa ccgtacgaat gccgtccccg ccctgggcat
     1441 ctacgcggtt aacatacgga gcaacgtatt ttgcccctgc cagcgcggcc agtaaccctt
     1501 gtgcagcact gtacactgcg gtgccaagcg tagtaatacc ctcttttttc agcatcttaa
     1561 tggcggccag accttcggaa gtcaccggga ttttcaccac aatgcccgga atggcgtcgc
     1621 gcaggcgctt cgcttcttcc accataccct gcgcgtcacg actcatagtc tgagcaaaca
     1681 gaatgccctc atcactaatc gctttttgca gacgcggcag aacttcccat atggactcct
     1741 tgctggcagc gataatgctc gggttagttg tcaccccggc aatggggaat atgcgtgcca
     1801 gacgttcgac ttctgcgacg ttagcggtgt ccagatacag ttccatgatg tttcctttta
     1861 cagttccagt tcatgttgca gcaggctggc gatagcgtcg gcagaggcgg cgttaaccag
     1921 cgcgttacgg aattcttcgt gcatgatgcg gcgagcgagg cgcgagaaaa tgcgcatgtg
     1981 ctgatcgccc gcagcgtgtt tgttcagggt taacataatg atgaattgcg cttcatcatc
     2041 gccccagcgc accggcgctt gcagacgcgc tacactgatg gtggattgct caatgtgttc
     2101 tgatttgctg tggggaatgg caaaactaaa gcccagaccg gtagagaaaa cggcctcgcg
     2161 cgcccacaag tcggcttcca gtttacgcgg atagcggcag cggcccgcca gcagcaggtt
     2221 atcggtcatg cctttgagca cttcttcttt gctgcgtcag tcgctttcca gcgtgatgca
     2281 ctcggcggtg accagcggtg cgtcctgttg ggtcatgcgg aattgcgcca gcaggtgttc
     2341 cacttccagc gaagtacggc aggccattgc ctggttgagc aactggcggc attcgcggct
     2401 atcaagttgc gccattcgcg ctttcgccgc cggaattgac ggtgcgctca tgctcagctc
     2461 atccagccct aaaccgacca gcaacggcag cacggaacct ttcgctccca gctcaccgca
     2521 cagaccaatc catttgccct ggcgatgcac cgcttgcacg gcgtaatcga gcgcccgcaa
     2581 gaatgccgga ttcaggctgt tgtagtgacg agtaacctta gcattatcgc gatccaccgc
     2641 cagcagatat tgcgtcaggt cattactacc aatactaaag aaatcaatct cttcgcagca
     2701 ttgatcgatg atgaacatca ccgacggcac ttccagcata atgccgagct ggatcttctc
     2761 atcaaacgga atgtgttcgt tacgcagttg ctgtttggct tccgccagtt tttctttcac
     2821 ccataagatc tcttccattg aagagatcat cgggatcatg attttcaggc tgccgtgagc
     2881 ggaggcgcgc aggatcgacc gtagttgtgt ggtgaacagc gaggcgtatt cttcataaat
     2941 acgcacagcg cgataaccga ggaacgggtt agcttctgcc ggaatgttca gataatcaac
     3001 gggtttgtcg ccgccaatgt ccatagtgcg cacaataatg ctgcgtccgt tggcagactc
     3061 cagcgcctga caaaaaatgt tgtacagctc gctttcgccc ggtgcactgg tgcgatccat
     3121 atagagcatt tcagtgcgga acaaaccaac gccttccgca ccattgccga atgcggcctg
     3181 cgcttccacg gagtgagcga tgttagcggc aatttcaatg cggataccgt cagcggtacg
     3241 ggcttgttgg gtcagccaga cacgctgttg ctcacgcagg gcatcctgta cgcgggcttc
     3301 ttgctgataa taacgggcta cggcttcccc tggctcaacc acaatcgctc cggcgttgcc
     3361 gtcgatataa atcgtttgat gttgccacgg ggtaagggca tcaatatcca caccaaccag
     3421 cgtcggaatg ttgaacgaac gggcaaggat caccgtatgt gaggtggtgc cgccgctttt
     3481 gagcaacaat cctttgaggt gatttttatc cagttcgagg aactggctgg gggtcagttc
     3541 atcagccata caaatggcgg gctgcgtcag tttgcccggt gccgggaagc gttgctcacc
     3601 gtagatttgc tggagtaact ggaagcagac gtcgcgtacg tccagggcac gttcttgcag
     3661 gtagctgctg ctggaacggg aaaactcttc acaaaagtga ttcgcactgg cgacaatcgc
     3721 ttcggcgcag cttaatccgg cgctgacgcc tgccagtaaa tgttcgtgca gggaagtatc
     3781 gccagccagg gatcggtgcg cttccagaat cgcgctggtg gcaccgtcgc tatccagcag
     3841 acgaaactca atgtttttca gtaccagcgt caggccgttt tccagtgcgg attgctcggc
     3901 gtcaacgtct ttggctgcgg gaagattact cagcgcattg agatctaaag aagagatcgg
     3961 cgtcagaatg ccgcctgcgc taccgctgca cacggtgcgg gcgcggataa tttgcggatt
     4021 cagattggtc agtgaaaccg gcagtggttc cagttcgtca gatttaactt ccgccagcgg
     4081 tgcgtcgcag tgggggaatt catcgcgcag ccattggctt aaacgctggt gcgcttcctg
     4141 ttcgtcggcc ccggaaatca gtaaccggca gttatcgccc gccagcgtgt cggtgccaat
     4201 cagcgcaagg gcgcttttgg cgttgccctt gcggtcagtg cgcaggttat gccactcaat
     4261 ttgtgatgaa aaagtattac acagcgtttc aacgtggctt gccggacgtg catgtacgcc
     4321 gttaggtagc tcacaaataa attccacaat cagggccatt gcctctctcc cggtacggtt
     4381 ttctgtttac aggataagag cgcacggcaa cggcctgcca tgtgacaaat ctgccaaaag
     4441 ctggacaaat gtaatgtaac cgtcaatttg cgacgcgtct cacaagacgc cgttttgcgg
     4501 catgcttccg gattatcgca agttatgagg cggatcgcat ttttgtactg atattacaaa
     4561 agtccagtaa atggcctttt tatccactgt ttgctccgcc tgcgattgcc tattgtttcc
     4621 tccatcaaaa atatggacat gggctttcgc aagcccggga gcagcttatg aatgagttgg
     4681 tgcagatcct gaaaaatacc cgtcagcatt taatgacggg cgtttcacac atgattccct
     4741 tcgtggtatc gggcggtatt ttgctggcgg tttccgtcat gttgtatggc aaaggcgcag
     4801 tgccggatgc cgtagccgat ccgaatctga aaaaactgtt tgatatcgga gttgcaggtt
     4861 tgacgctgat ggtgcctttc ctcgccgctt acatcggtta ctccattgca gagcgttctg
     4921 cgctggcccc gtgcgctatc ggtgcctggg ttggtaacag ctttggtgcg ggcttctttg
     4981 gtgcgctgat cgccgggatt atcggcggca tcgtggtgca ttacctgaag aaaattccgg
     5041 tgcataaagt tctgcgctcg gtgatgccta tcttcattat tcctatcgtc ggcacactga
     5101 ttaccgcagg catcatgatg tggggcttgg gcgagcctgt aggggcgttg accaacagcc
     5161 tgactcagtg gcttcagggg atgcagcagg gcagcattgt tatgctggcg gtgatcatgg
     5221 gtctgatgct ggcgttcgat atgggcggtc cggttaacaa agtggcctat gccttcatgc
     5281 tgatttgcgt tgctcagggt gtttataccg tggtggccat tgctgccgtg ggtatttgta
     5341 tcccgccgct ggggatgggg ctggcgacgc tgattggtcg taaaaatttc tccgcagaag
     5401 agcgcgaaac cggcaaagcg gcgctggtga tggggtgcgt tggggttact gaaggggcga
     5461 ttcctttcgc cgctgccgat ccgctgcgtg tcattccttc catcatggtc ggttctgttt
     5521 gtggtgcggt aactgcggcg ctggtcggtg cgcagtgcta tgcaggctgg ggtggtctga
     5581 ttgtgctgcc ggtggttgaa ggcaagctgg gttatatcgc agcagtggct gtcggagcag
     5641 tggtgacggc tgtttgcgtg aacgtgctga aaagtctggc gcgtaaaaat gggtcttcga
     5701 ctgatgagaa ggaagacgac ctggatttgg attttgaaat taattaattg aggaacgagc
     5761 catgacgaaa attattgcag taaccgcgtg tccatctggt gttgcccata cctatatggc
     5821 ggcagaggcg ctggaaagcg ccgcgaaagc gaaaggctgg gaagtgaaag tagaaactca
     5881 ggggtcaatt ggtctggaaa acgaactgac tgcggaagac gtggcgagcg ccgatatggt
     5941 gattctgacc aaagatatcg gcatcaagtt tgaagagcgt tttgctggca agaccatcgt
     6001 gcgcgtcaac atcagcgatg cggtaaaacg tgctgacgcc attatgagca aaattgaagc
     6061 tcacctggcg caaaccgctt aagttcttct cccgctcgca agggcgggtt cgctttccca
     6121 caggagttcc tcatgacgaa tcgtatctct cgcctcaaaa ctgcactgtt ttccaatacc
     6181 cgtgaaatct cgctggagcg ggcgctgctt tataccgcca gccatcggca aaccgaaggc
     6241 gaaccggtga tattgcgccg ggcgaaagca acagcgtata tccttgaaca tgttgaaatt
     6301 tcgattcgtg atgaagaact gattgccggt aaccgcaccg taaaaccgcg cgccgggatt
     6361 atgtcgccgg aaatggaccc ttactggctg ctgaaagagc tggatcaatt cccgacgcgt
     6421 ccgcaggacc gctttgctat cagcgaagaa gataaacgta tctaccgcga agagttgttc
     6481 ccgtactggg aaaaacgttc gatgaaagat ttcatcaacg ggcagatgac agatgaagta
     6541 aaagccgcga ccagcacgca gattttcagc attaaccaga cggataaagg ccaggggcac
     6601 attattattg attacccacg cctgctgaat cacgggctgg gggagctggt ggcgcagatg
     6661 cagcaacatt gtcagcaaca gccggagaat cacttttatc aggccgcgct gttactgctg
     6721 gaagcctcgc agaaacacat tctgcgttac gctgaactgg cggaaacaat ggcggcaaac
     6781 tgcacagatg cccagcgtcg cgaagaactg ctgactattg cggagatctc ccgccataac
     6841 gccgaacata agccgcagac gttctggcag gcgtgccagt tattctggta catgaacatc
     6901 attctgcaat acgaatccaa cgccagttcg ctgtctttag ggcgcttcga ccagtatatg
     6961 ttgccgttct atcaggcttc tttaacccag ggcgaagatc cggcgttcct gaaagaactg
     7021 ctcgaatctt tatgggtgaa atgcaacgac atcgtgctgt tgcgctccac cagcagcgcg
     7081 cgttatttcg caggtttccc gaccggctat accgcactgc tcggcgggtt aaccgagaac
     7141 ggacgtagcg cggtgaacgt gctttcgttc ctttgccttg acgcctatca aagcgtgcaa
     7201 ttaccgcaac cgaacctcgg cgtgcgcact aacgccttga tcgacacgcc gttcctgatg
     7261 aaaaccgccg aaaccattcg cctcggcacc ggtattccgc aaatctttaa cgatgaagtg
     7321 gtggtaccag cgttcctcaa ccgtggcgtt tcgctggaag atgcgcgcga ctattccgta
     7381 gtgggctgtg tggaattatc tattcccggc agaacctacg gcttgcatga catcgcgatg
     7441 tttaatctgc tgaaagtgat ggaaatctgc ctgcatgaaa atgaaggcaa tgctgcgctg
     7501 acttatgaag gtttactgga gcagatccgc gccaagatca gccactacat caccctgatg
     7561 gttgagggca gcaatatttg tgatatcggc catcgcgact gggcacctgt accgctgctc
     7621 tcgtctttta tcagcgattg tctggaaaaa ggtcgcgata ttaccgatgg cggcgcgcgt
     7681 tataacttct ccggcgtaca ggggatcggt atcgccaacc tgagcgattc tctccatgcg
     7741 ttgaaaggga tggtttttga gcaacagcgt ttaagttttg acgaattgct gtcggtatta
     7801 aaagccaact ttgcaacgcc agaaggcgaa aaagtccgtg ctcgcttaat taaccgcttc
     7861 gagaaatacg gtaacgatat cgacgaggtg gataacatca gcgccgaact gttacgccac
     7921 tactgcaaag aagtggaaaa ataccagaac ccgcgcggtg gttacttcac gccgggatcg
     7981 tataccgttt ctgctcacgt cccgttggga tcggtggttg gcgcgacgcc agacggtcgt
     8041 tttgccgggg aacaactggc agacggcggc ttgtcaccta tgctgggcca ggacgcacaa
     8101 gggccaacag cggtactgaa gtcagtcagt aagctcgata atacgctgct gtctaacggt
     8161 acgttgctga acgtgaaatt cactccggcg accctggaag gtgaagcggg attacgcaaa
     8221 ctggccgact tcttacgggc gtttacccag cttaagttgc agcatattca gtttaacgtg
     8281 gtgaacgccg acacgttgcg ggaagcgcaa cagcgcccac aagattatgc cgggctggtg
     8341 gtgcgcgttg ccggatacag cgccttcttt gtcgaactgt cgaaggagat ccaggatgac
     8401 atcatccgcc ggacagcgca tcagctgtaa cgttgtggaa acgcgccgca atgatgtggc
     8461 gcgcattttc aacattcagc gttattcact gaatgacggc gagggcattc gtacggtggt
     8521 cttttttaaa ggctgtccgc atctttgccc atggtgtgct aatccggagt cgatctccgg
     8581 caaaatccag acggtacgca gagaggcgaa atgtctgcac tgtgcgaaat gtttgcgtga
     8641 tgcggatgaa tgcccctccg gggcgtttga acggattggt cgcgatatca gccttgacgc
     8701 gctggaacgg gaagtgatga aagatgacat tttctttcgc acgtccggcg gcggcgtcac
     8761 gctttctggc ggcgaagtgt taatgcaggc ggagtttgct acccgttttt tacagcggct
     8821 gcggttgtgg ggcgtgtcat gcgccattga aactgccgga gacgtgccag ccagcaagct
     8881 gttaccactg gcgaaattgt gcgatgaagt gctgttcgat ttaaaaatta tggacgcgac
     8941 tcaggcgcgg gatgtggtga agatgaacct gccacgcgtg ctggagaatc tgcgtttgct
     9001 ggtgagtgag ggtgtcaacg tgatcccgcg tttaccgttg atccctggtt tcacgctcag
     9061 ccgggagaat atgcagcagg cgctggatgt actgatcccg ctgaaaatca ggcagatcca
     9121 tctgttaccg tttcatcagt acggcgaacc gaaataccgc ctgctgggga aaacatggtc
     9181 gatgaaagag gtgcctgcgc cgtcgtcagc cgatgtgaca acgatgcgcg aaatggcaga
     9241 acgggccgga tttcaggtta ccgtgggagg ttaaaatggc atacctggtg gcagtaaccg
     9301 cctgcgtaag cggcgtggcg catacttata tggcggcgga acggctggaa aagttgtgcc
     9361 agttagagaa gtggggcgtt agcattgaaa ctcagggcgc gctgggaacg gagaatcgtt
     9421 tagcggacga ggatatccgt cgggcggatg ttgctctgtt gattacggat atcgagcttg
     9481 ccggtgccga gcgatttgaa cattgccgct atgtgcaatg cagcatctac gcattcctgc
     9541 gtgagccgca gcgggtaatg agcgcggtgc gcaaagtgct ttctgcgccg cagcaaaccc
     9601 atcttattct ggagtagtcg gtttttctgt cagttggctg tggtactgcc gacgatattc
     9661 cgacggcgag cgttcggtgt ttttacgaaa cagacggcag aagtagttgc tgtcgacaaa
     9721 gccgcaggcg tgcgccactt cttttacctt caggtcatag cctttcaata acgtcttagc
     9781 gtgctccagt cgcgtgtgat tcaggtattc gttaaaacca atggcccccg ttttttgaaa
     9841 caggtgcgag agatagtttg gtgaaatata gaatgcctgt gcgacagatt cgcgggtaag
     9901 cgcggaggca tagcgttcgt cgatataatc gcgaatagct tcaaatagtg cctggctgcg
     9961 tgaggcggtc tggatttggc tgccgagcaa atcgcggcag tggctaagca ggctggcgac
    10021 gataagcctt gccgtttgct gctcctgcgg ctgcatttgc atttcattga gcgtttgtag
    10081 cagaaaagaa cctatgcgtg ggccgcgccg ggcgacgtgt tgcttcgcca gattttgata
    10141 ttgtttgcca tcccattgca cgacgctgaa gccgagttgc tgtttgccaa acagcacgct
    10201 aaaggtagta gcgggggctt gccattgtgg aaaattccag ccaccagcag gaacgtacag
    10261 cacatcgccg ggaactaacg ttgctccagc gccggtatca acaaactcac cttccagcac
    10321 aatttccagc cgtggaaaat cgacctgata cgcgagatcg ggcaccgggc cgttactgct
    10381 ggcaaagtaa atctggcgca gggacagcgg gccgttgatc aggcgggaga gcagatagct
    10441 gacgtcgtga tacatgttat tcccttaggt atgtagacgg gaaaacagta actggcatta
    10501 ttaaaaaagg ccacctgaaa agtgtggcct gaggggagtt cgatgtaaac attgcggcag
    10561 tatcgcatcc ggcaaatagc gccagacgcg atacaaaggt catttactga ttacccacct
    10621 gatcgccata cggcagcgtg tcgtaatcga tcagcgcgtt tttcttgtac gggttaccaa
    10681 tccagcgggt agtttctttg aactgcggat tcaccactga acgggttgga tcgtaaccgt
    10741 cgtaagataa gcctgctaaa tctgaccagg tgtggatcag ttccgccagg ctgtatttac
    10801 gatcaacatc ctgcgagaaa tcacgcggat gagtcgcttg ccatttttcc gacgtccaca
    10861 gcaggaacgg aatggtgtac atgtggcgcg tcgggttgtc ttcgttacgc ccctgagttt
    10921 tgtgcggcgg cgtgtcgtaa acctcttcac cgtggtcaga gaaataaacc aggaaaccgt
    10981 tcgggtttgc tgctttaaag tctttaatca ggctggcaac cacatgatcg ttatacaggt
    11041 tagcgttgtc ataatcgtta tatgactcca gctcttccgc gtttaatcca ggcggaacat
    11101 gatcggtatt gccatcaaac ttgccctgat tttccgggta gcggtatttg tatttgatat
    11161 gcgtacccag cagatgaacg atgatcagtt tcttcggcgc cgggtccttc agcacttcct
    11221 ggaatggttt cagcacgttg gtgtcgtatt cacgcgcact ctgcgtacgt tgctggttca
    11281 tatagaactg tttgtcggtc tgcttcgaaa ataccgtcag catggtattg cgggcggtca
    11341 tcgtctgctg gttggtgatc cagaaggttt tataacctgc ctgtttcatc atgttcatca
    11401 gcgacggttg tgtcaggtac aggtccgggt tcttttcatt agcaaaggtc agcgcctgtt
    11461 gcaggatttc aatggtgtac ggacgagacg taaccacgtt attaaacacg gtcagattcg
    11521 gatcggtttt atgcagcgca tccagctccg gcgtggtttc acgcggataa ccgtacagac
    11581 tcatgcgccc gcgttgggtc gattcgccaa tcaccagcac taaggtacgc ggttcgttac
    11641 ccgattcatc tttgaaatta gccagaggcg gcaaggcatt attttcattc agtaactttg
    11701 ttagcgagtt tagttgctga cgatactgat aatagccggt caggaattgc cacggtgcgg
    11761 caggctccat acgcgaggcc aggttatcca acgttttctc aaacggcttg tttttgataa
    11821 acgtattcat ggcgatcgga tgcagaatca agccataaag cagggcaaaa gagacaacat
    11881 aacgccacgg ctttggaata tagaccgggt gcaggcgtgt ccacagcaga actgccaccg
    11941 ccgtataggc cagcgcgata agcacaattt tcaggctgaa atactggctt aaatactcgc
    12001 tggcttcgtt ggtgttggtt tcgaacatca caaacagaac gctctgcgag aactcctgcc
    12061 cgtagatgac gtagtagcat agcgccgcca gagaggccgc ccatagcacc acgccgatta
    12121 ctgcggcaat aattttaatc cgcttcggaa agaggaatac cgggatcaac cacagcgagc
    12181 tgaataacag cgagtcgcga atgccgttgg tgccactata accactgatg taaataatgg
    12241 cctgtagcag agtagagaaa aaccaaaagt agagcagtgc ccaacccagg gctttccagc
    12301 taaaaagggg tttagcctgg acttctgtgg aatgcatagt aagaacctgt cttgaaaaaa
    12361 atatcgccga atgtaacgac aattccttaa ggatatctga aggtatattc agaatttgaa
    12421 taaaatgcag acagaaatat attgaaaacg agggtgttag aacagaagta tttcagaaaa
    12481 ccctcgcgca aaagcacgag ggtttgcaga agaggaagat tagccggtat tacgcatacc
    12541 tgccgcaatc ccggcaatag tgaccattaa cgcttgttcg acgcgaggat ccggttcctg
    12601 gccttctttt tctgcctggc gggagcggtg cagtaactcg gcctgcaata cgttcagcgg
    12661 gtcggtgtaa atattccgta gctgaataga ctctgcaatc cacggcagat cggccattag
    12721 atgggaatcg ttggcaatcg ccagcaccac tttgatgtct tcttcttgca ggttgcgtaa
    12781 ctctttacct aacggccaca gtgctttgtc taccaggcgt tggtcatagt attccgccag
    12841 ccacaggtct gctttggcga agaccatctc cagcatgccg agacgcgtcg agaagaatgg
    12901 ccaatcgcgg cacatggctt ccagctcgct ctgtttgcca tcttcgacca ctttttgcag
    12961 cgccgtacct gcacccagcc aggcggggag catcaggcgg ttttgcgtcc aggcaaaaat
    13021 ccacggtatg gcgcgcagcg actcgacgcc gccggttggg cgacgtttcg ccggacgtga
    13081 acccaacggc agtttgccca attcttgttc cggcgtagcg gagcggaagt aaggcacaaa
    13141 atctttgttt tcacgtacgt agccacggta gagatcgcag gagatgactg acagttcatc
    13201 cataatgcga cgccagctct ctttcggctc aggtggtggc agcaggttgg cttccagaat
    13261 agccccggtg tacagcgaca ggctgctgac ggtgatttct ggcagaccat atttaaagcg
    13321 gatcatctcg ccctgttcag tcacgcgcag gccacctttc aggcttcctg gcggttgtga
    13381 cagcagcgcc gcatgagcag gtgcgccgcc gcgaccaatg gaaccgccgc gaccgtggaa
    13441 caacgtcagc tcaatacccg ctttttcgca ggttttgatt aatgcatcct gtgcctgata
    13501 ttgcgcccag gaagctgcca tcactcccgc atcttttgct gagtcggaat agccaatcat
    13561 caccatctgt ttgccctgaa tcaggccacg ataccagtca atattgagca gctgggtcat
    13621 gacatcgttg gcgttgttca gatcatcgag ggtttcaaac agcggagcaa ccggcatcgc
    13681 aaacccgata cccgcttctt tcagcagcag gtggacagcc agtacgtcgg acggcgtttt
    13741 cgccatcgag atcacgtagg cggcaatgga gccttgcggt gcttcggcaa tcacctggca
    13801 ggtatcgagc acttcgcgcg tttcggcgct tggttgccag ttgcgcggca gaagcggacg
    13861 tttggagttc agttcgcgga tcaggaacgc ctgtttgtcg gcctctgacc agctttcgta
    13921 gtcgccgata ccgaggtagc gggtcagctc gcccagcgct tcggtatgac gcgtgctctc
    13981 ctgacggata tcaatacgga ccagcggtac gccgaaacat ttcacgcggc gcagggtgtc
    14041 gagcagatcg ccgttggcga taatacccat gccacacgcc tgaagtgact ggtagcaagc
    14101 gtagagcggt tcccacagtt cttcgttttg tgtcagcagg ccttctggtt ttggcagttc
    14161 ttcgcctttc aggcgcgctt ccagccatgc ctgtgtcgcc atcaggcgag aacgcaggtt
    14221 tttcatcaga tagcgatacg gttctgcggc accttcttcg ccaaccagcg ccagcagttc
    14281 aggggtcgct tcaaccatcg acagttcaga aaccagcacc tgaatatctt tcaggaacaa
    14341 atcggtggct ttccagcggc tgagtagcag gacgtggcgg gtgatatcgg cagtgacgtt
    14401 cgggttgccg tcgcggtcgc cgcccatcca cgaagtaaaa cggaccggaa caaattcgac
    14461 gggcagtttg tagccgaggt tctcttccag ttgttcgttc agttcgcgca ggtaatttgg
    14521 tacgccttgc cacaggctgt tttccactac ggcaaagccc catttggctt catctaccgg
    14581 gcttggacgc agcttacgga tttcatcggt atgccatgac tgggcgatta actggcgcag
    14641 gcggcgcatc agctggttgt gttcgtagtc agcgatatct ttgttatcga gctgttttaa
    14701 acaggcgttc acttccacca ttttgtggat cagtgtacga cgggtaattt cggttgggtg
    14761 agccgtgagg accagttcca gcgacagcga ttccactgct tttttgatgg tgtcttcgct
    14821 cagttccggc tggtttttca gtttacgcag ggtgcgggcg atcacttccg ggttgctggc
    14881 agcttcgcct ttcggcgaaa tgctgtggta ttgctcggcg gtgttggcca ggttcaggaa
    14941 ctgactaaac gcacgcgcaa cgggcagcag ctcgtcgttc gacaaatttt gtaaggtggt
    15001 gagcaactcc tggcggttag catcattgcc agcgcgtgaa gatttcgaca acttacggat
    15061 agtttctacg cgttcaagaa tgtgttctcc caacgcatcc ttgatggttt ctcccagcac
    15121 tttgccgagc atactgacat tactacgcaa tgcggaatat tgttcgttca tattacccca
    15181 gacaccccat cttatcgttt gatagccctg tatccttcac gtcgcattgg cgcgaatatg
    15241 ctcgggcttt gcttttcgtc gtcttttata aagccacgta aaagcggtga cgtcaaatgc
    15301 tgcgaaatcg cttcagcaaa cgaataaata gcaggaattt acgccattaa attcacgacg
    15361 ctttaaataa gcgtaactta tggaaatgtt aaaaaatcgc catacataac accaaaggtg
    15421 taggtcggat aagatgcgca agcatcgcat ccgacattat tgcggcacag gagtttggca
    15481 acggtgccgg atgcggcgcg agcgccttat ccggcctacg ttttaatgcc agcaaaaatg
    15541 gtgaattacc tgggttatca gttcgcgggt gggcttgata aaccgtgttt ccagatattc
    15601 atcaggttga tgagcctgat taattgagcc aggccccaac accagcgtcg ggcataacgt
    15661 ttgaataaac ggcgcttcgg tacagtagtt caccacttcg gtctttgctc cgagcaattt
    15721 ctcaactact tcaaccagtt gatgattcgg tgggcattca tagccaggga tcggcggatg
    15781 cagctcgtcg accgtcagac gacccggcca gcgttcgctc accggagcca atgcatcgtt
    15841 gagcaaacca ttaagttcat tgagtgtcat gccaggcagc ggacgaatat ccatatgcaa
    15901 ctcacagcaa gcgcaaatac ggttagaagc gtcgccaccg tgaatatgcc cgaggttgag
    15961 cgtagggtat ggcacggtaa acgcttcgta gtgataacgt tctttcaggt tatcgcgcaa
    16021 ttgcaaaata tgcccgatgg cgtcgtgcat tagttcgata gcgttaactc cgcgtgctgg
    16081 atcgctggag tgccccgact ggccctgaat acggatggcg ttagagatat gacctttatg
    16141 tgcgcgtacc ggttgtagtg acgtcggttc gccaatgatg gcgcaatccg ggcgcagggc
    16201 ggtagtttcg gcaaaataac gcgctccggc catactggtt tcttcatcag cagtcgccag
    16261 aatgtagagc ggttttttca gtttcgtgac gtcgacatcg cgtagcgcat caaggataaa
    16321 cgcaaaaaag cctttcatgt cggcggtgcc taagccgtaa agcttgccgt catgctccgt
    16381 cagtgtaaac ggatcgcgcg tccagcgacc gtcatcaaat ggcaccgtat cggtatgccc
    16441 cgccagcaac aagccgccag ccccctgtcc gatacttgcc agcatattga atttgttgcg
    16501 agttcctggc acaggctgca cttccacatt gaagcctaaa tctttaaacc agtccgccag
    16561 cagagtgatt aaatctgcat tgctttgatc gagtgcctct tccgtggcgc ttattgaagg
    16621 tgtggcaatc agagcacggt aaatctcgat aaatggcggt aatttgtttt tcattgttga
    16681 cacacctctg gtcatgatag tatcaatatt catgcagtat ttatgaataa aaatacacta
    16741 acgttgagcg taataaaacc caccagccgt aaggtgaatg ttttacgttt aacctggcaa
    16801 ccagacataa gaaggtgaat agccccgatg ttgaatacgc tgattgtggg tgccagcggc
    16861 tacgctggcg cagagctagt gacctatgta aatcgccatc cgcatatgaa cataaccgct
    16921 ttgactgttt cagcgcaaag caatgatgcg ggaaagttaa tctccgattt gcatccgcag
    16981 ctaaaaggca tcgttgatct gccgttgcag ccgatgtcgg atatcagcga gtttagccca
    17041 ggggtggacg tagtgtttct cgccaccgcc catgaagtta gccacgattt agcgccgcag
    17101 tttcttgaag cgggctgcgt ggtgttcgac ctttccggcg cgtttcgtgt taacgacgcc
    17161 accttctatg aaaaatatta cggctttacc catcaatacc cggaactgtt ggaacaggca
    17221 gcctacggtc tggcggagtg gtgcggtaat aaattaaaag aagcgaattt gattgcggtg
    17281 ccgggctgtt atccgacggc ggcacagctg gcgctgaaac cgttgattga tgccgatctt
    17341 cttgacctca atcagtggcc ggtgatcaac gccaccagcg gcgtgagcgg tgcaggacgt
    17401 aaggcagcga tttcaaatag tttttgtgaa gttagcctgc aaccgtatgg cgtctttacc
    17461 catcgccatc aaccagagat cgccacacac ctcggtgctg acgttatctt caccccgcat
    17521 ctgggcaatt tcccgcgcgg cattcttgaa accattacct gccgcctgaa accgggtgtg
    17581 acccaggcgc aagtggcgca agcgttacag caggcgtatg cccataaacc gctggtgcgg
    17641 ctgtatgaca aaggcgttcc ggcgctgaaa aatgtcgttg ggctgccatg ttgcgatatc
    17701 gggtttgccg ttcagggcga gcatctgatt attgtggcga ccgaagacaa cttactgaaa
    17761 ggcgcggcgg cacaagcggt acagtgcgcc aatattcgtt tcggctatgc ggaaacgcag
    17821 tctcttattt aagggtgcaa tgatgaatcc attaattatc aaactgggcg gcgtactgct
    17881 ggatagtgaa gaggcgctgg aacgtctgtt tagcgcactg gtgaattatc gtgagtcaca
    17941 tcagcgtccg ctggtgattg tgcacggcgg cggttgcgtg gtggatgagc tgatgaaagg
    18001 gctgaatctg ccggtgaaaa agaaaaatgg cctgcgggtg acgcctgcgg atcagataga
    18061 cattatcacc ggagcactgg caggaacggc aaataaaacc ctgctggcat gggcgaagaa
    18121 acatcagatt gcggccgtag gtttgtttct cggtgacggc gacagcgtca aagtgaccca
    18181 gcttgatgaa gagttaggtc atgttggact ggcgcagcca ggttcgccta agcttatcaa
    18241 caccttgctg gagaacggct atctgccggt ggtcagctcc attggcgtaa cagacgaagg
    18301 gcaactgatg aacgtcaatg ccgaccaggc ggcgacggcg ctggcggcaa cgctgggcgc
    18361 ggatctgatt ttgctctctg atgtcagcgg cattctcgac ggcaaagggc aacgcattgc
    18421 cgaaatgacc gccgcgaaag cagaacaact gattgagcag ggcattatta ctgacggcat
    18481 gatagtgaaa gtgaacgcgg cgctggatgc tgcgcgcacg ttgggccgtc cggtagatat
    18541 cgcctcctgg cgtcatgcgg aacagcttcc ggcactgttt aacggtatgc cgatgggtac
    18601 gcggatttta gcttaagttt tgttggccgg aggcgcagct ttccggcatt gaatttcaaa
    18661 ataaggaaac agagttatgg cactttgggg cgggcgtttt acccaggcag cagatcaacg
    18721 gttcaaacaa ttcaacgact cactgcgctt tgattaccgt ctggcggagc aggatattgt
    18781 tggctctgtg gcctggtcca aagccctggt aacggtcggc gtgttaaccg cagaagagca
    18841 ggcgcaactg gaagaggcgc tgaacgtgtt gctggaagat gttcgcgcca ggccacaaca
    18901 aatccttgaa agcgacgccg aagatatcca tagctgggtg gaaggcaaac tgatcgacaa
    18961 agtgggccag ttaggcaaaa agctgcatac cgggcgtagc cgtaatgatc aggtagcgac
    19021 agacctgaaa ctgtggtgca aagataccgt tggcgagttg ctgacggcta accggcagct
    19081 gcaatcggcg ctggtggaaa ccgcacaaaa caatcaggac gcggtgatgc caggttacac
    19141 tcacctgcaa cgcgcccagc cggtgacgtt cgcgcactgg tgcctggcct atgttgagat
    19201 gctggcgcgt gatgaaagcc gtttgcagga tgcgcttaag cgtctggatg tcagcccatt
    19261 aggctgtggc gcgctggcgg gaacggccta tgaaatcgac cgtgaacagt tagcaggctg
    19321 gctgggcttt gcttcggcga cccgtaacag tctcgacagc gtttctgacc gtgaccatgt
    19381 gttggaactg ctttctgctg ccgctatcgg catggtgcat ctgtcgcgtt ttgctgaaga
    19441 tctgattttc tttaacaccg gcgaagcggg gtttgtggag ctttctgacc gcgtgacttc
    19501 cggttcatca ttaatgccgc agaagaaaaa cccggatgcg ctggagctga ttcgcggtaa
    19561 atgcggtcgg gtgcaggggg cgttaaccgg catgatgatg acgctgaaag gtttgccgct
    19621 ggcttacaac aaagatatgc aggaagacaa agaaggtctg ttcgacgcgc tcgatacctg
    19681 gctggactgc ctgcatatgg cggcgctggt gctggacggc attcaggtga aacgtccacg
    19741 ttgccaggaa gcggctcagc agggttacgc caacgccacc gaactggcgg attatctggt
    19801 ggcgaaaggc gtaccgttcc gcgaggcgca ccatattgtt ggtgaagcgg tggtggaagc
    19861 cattcgtcag ggcaaaccgc tggaagatct gccgctcgac gagttgcaga aattcagccc
    19921 ggtgattgac gaagatgtct atccgattct gtcgctgcaa tcgtgcctcg acaagcgtgc
    19981 ggcaaaaggc ggcgtctcac cgcagcaggt ggcgcaggca attgcttttg cgcaggcgcg
    20041 gttggggtaa gaacatttat atgtataaat ttgagcctgg cttatcgccg ggctttttta
    20101 tggcaaaaaa aagcggatcc tggagatccg caaaagttca cgttggcttt agttattcga
    20161 gttgagaaac tctcgaaacg ggcagtgact tcaagggtta aaagaggtgc cgctccgttt
    20221 ctgtgagcaa ttatcagtca gaatgcttga tagggataat cgttcattgc tattctacct
    20281 atcgccatga actatcgtgg caatggagga tggataatga atattcgtga tcttgagtac
    20341 ctggtggcat tggccgaaca ccgccatttt cggcgtgcgg cagattcctg ccacgttagc
    20401 cagccgacgc ttagcgggca aattcgtaag ctggaagatg agttgggcgt gatgttgctg
    20461 gagcggacca gccgtaaagt gttgttcacc caggcgggaa tgctgctggt agatcaggcg
    20521 cgtaccgtac tgcgtgaggt gaaagtcctt aaagagatgg caagccagca gggcgagacg
    20581 atgtccgggc cgctgcacat tggtttgatt cccacagttg gaccgtacct gctaccgcat
    20641 attatcccta tgctgcacca gacctttcca aagctggaaa tgtatctgca tgaagcacag
    20701 acccaccagt tactggcgca actggacagc ggcaaactcg attgcgtgat cctcgcgctg
    20761 gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg atgagccaat gttgctggct
    20821 atctatgaag atcacccgtg ggcgaaccgc gaatgcgtac cgatggccga tctggcaggg
    20881 gaaaaactgc tgatgctgga agatggtcac tgtttgcgcg atcaggcaat gggtttctgt
    20941 tttgaagccg gggcggatga agatacacac ttccgcgcga ccagcctgga gacgctgcgc
    21001 aacatggtgg cggcaggtag cgggatcact ttactgcctg cgctggctgt gccgccggag
    21061 cgcaaacgcg atggggttgt ttatctgccg tgcattaagc cggaaccacg ccgcactatt
    21121 ggcctggttt atcgtcctgg ctcaccgctg cgcagccgct atgagcagct ggcagaggcc
    21181 atccgcgcac gaatggatgg ccatttcgat aaagttttaa aacaggcggt ttaaaccgtt
    21241 taacgcagct acccgatagg cttccgccat cgtcgggtag ttaaaggtgg tgttgacgaa
    21301 gtactcaata gtgttgccgc cacctttctg ttccataatc gcctgaccga tatgaataat
    21361 ttcggcagcg cgctcgccaa agcagtgaat acccagaatc tcttttgttt cccgatggaa
    21421 caaaattttc agcgtgccca cgttcatgcc gacgatttgt gcgcgtgcca gatgtttaaa
    21481 ctgggcgcgg cccacttcat atggcacttt cattgcggtc agctgctgtt cggttttgcc
    21541 cacagagctg atttccggga tggtgtaaat gccggtaggg atatcttcaa tcagatgtgc
    21601 ggtggcttcg ccttttacca gcgcctgcgc ggcaatgcgc ccctggtcat aggccgctga
    21661 tgccaggctc ggataaccaa tcacgtcgcc caccgcgtaa acgtgcggct gtgcggtctg
    21721 atacatgctg ttgaccttca gctgtccgcg gctgtcagtt tccagcccaa tgttctgtaa
    21781 cgccagcgaa tcggtattac cggtgcgacc gttggcatag agcaggcagt cagctttcag
    21841 ttttttaccc gacttcaaat gcatgatcac accatcgtca cagccttcga tcttctcgta
    21901 ctcttcgttg tgacgaatca ctacgccact gttccagaag tgataggaga gagaatctga
    21961 catctcttga tcgagaaatg ccagcaggcg atcgcgggtg ttgatcagat ccacttttac
    22021 atccataccg cggaagatcg acgcatattc acagccgatc actccagcac catagataag
    22081 tacatggcgc ggttcgtggt gcatgctaag aattgagtcg ctgtcgtaaa tgcgtggatg
    22141 ggtgaaatca acatctgttg gatgatatgg acgagagccg caggcaataa caaatttttc
    22201 agcggttagt gtttcaacgc tgccgtccgg gcaatccagc gccaacgtat gctcgtcaac
    22261 aaagcgagcg tttccctgca atatttcaca gtgattacgt tcgtaaaatc cctgacgcat
    22321 gcgcgtttgt tgattaatca cgttatcggc atggttaagg atatcggcaa aagaagagcg
    22381 gagcagtcgg gaatggtcgc tgtaaagtgg gttttgattg aattctataa tgcggctgac
    22441 ggcgtgacgg agagctttcg acgggatggt gccccagtgg gtgcaaccgc cgccaacatt
    22501 ttgataacgc tcgataactg cgacgcgcgc accttgctta accaggccca ttgcagcgcc
    22561 ttcgccgccg gggccggaac ctattactat ggcatcgtaa tcgtaggaat gtggcatggt
    22621 agggcttacc tgttcttata cataaaagca acagaatggt aacattttat cgcgggtaag
    22681 ccaattgatc cccgtcattt atctggctat atcctgagcg gcctttgctt tgtctgtttc
    22741 ttacttttgc cctgacgttt tattggattt ttatcgacga tactctccgt ttaagcggca
    22801 ggtttccgct gtacgtaaaa gaaccggcca aagaattgca gtaaatatgt tttattgcgt
    22861 taccgttcat tcacaatact ggagcaatcc agtatgttca ttctctggta tagtgccagc
    22921 agtacttttg gcaaggattc agacatcgtg atgggcgtaa gagcgcaaca aaaagaaaaa
    22981 acccgccgtt cgctggtgga agccgcattt agccaattaa gtgctgaacg cagcttcacc
    23041 agcctgagtt tgcgtgaagt ggcgcgtgaa gcgggcattg ctcccacctc tttttatcgg
    23101 catttccgcg acgtagacga actgggtctg accatggttg atgagagcgg tttaatgcta
    23161 cgccaactca tgcgccaggc gcgtcagcgt atcgccaaag gcgggagtgt gatccgcacc
    23221 tcggtctcca catttatgga gttcatcggt aataatccta acgccttccg gttattattg
    23281 cgggaacgct ccggcacctc cgctgcgttt cgtgccgccg ttgcgcgtga aattcagcac
    23341 ttcattgcgg aacttgcgga ctatctggaa ctcgaaaacc atatgccgcg tgcgtttact
    23401 gaagcgcaag ccgaagcaat ggtgacaatt gtcttcagtg cgggtgccga ggcgttggac
    23461 gtcggcgtcg aacaacgtcg gcaattagaa gagcgactgg tactgcaact gcgaatgatt
    23521 tcgaaagggg cttattactg gtatcgccgt gaacaagaga aaaccgcaat tattccggga
    23581 aatgtgaagg acgagtaatg aaacaagcaa atcaagatag aggtacgctg ctgctggcgt
    23641 tagttgctgg cttatcgatt aatggtactt tcgcagcgct gtttagctcc attgtgccat
    23701 tttctgtatt cccgattatt tccctggtgc tgacggttta ctgcctgcat caacgttatc
    23761 ttaatcgcac tatgccggta ggcttgccgg gtctggcagc tgcctgtttt attctcggcg
    23821 tactgctgta cagcacggta gttcgtgcgg aatatccgga tatcggctct aacttcttcc
    23881 cggcagtact ctccgtcatt atggtgttct ggattggcgc gaagatgcgt aaccgtaagc
    23941 aggaagttgc tgaataatcg gtattatgcc ggatgctggc gcatccagca tgggttttac
    24001 ttcgcggtca gtaatacgcc gcactccata tggtgcgtgt aggggaactg atcaaacaga
    24061 gccagacgtt cgaccttgtg cgtctggctt aatgtttcca gattcttgca taacgtttcc
    24121 gggttacagg agatgtacaa aatacacgga tacgcctgca ccattttctc ggtttcactg
    24181 tccagaccgc tgcgcggtgg gtcgacaaaa atggtttcgc actgataact ctttaagtcg
    24241 atcccttgca ggcggttaaa ctcgcgcaca ccattcatcg cctgagtaaa ttcttctgcc
    24301 gccatacgaa taatttgtac gttatcaata tggttagctg cgatgttgta ttgcgcagca
    24361 gcaaccgacg gcttagcgat ttcggtggct aatacccgat caaaattgcg cgccagcgct
    24421 aatgaaaagt taccgttgcc gcagtacagc tccagtaaat cgccttttga gcctttggtt
    24481 acgtccagcg cccattccag catctgaata ttcatcgccg cgttcggctg ggtaaagctg
    24541 ttttctacct gacggtagat catctctttc cctgcgaccg gcagacgctc gtcgatgtaa
    24601 tcttgatcca gctcgatttt ggttttcgtt gcccgaccaa tcagatgcac attcagattc
    24661 tgcgcgcgca gtgcatcgcg cagggcctcc gcttcctgac gccactcatc atccagcttc
    24721 ttatggtata gcagggaaac caccgcctga ttactcagcg tggtgaggta atcaatctgg
    24781 aacaacttgt ggcgcagaac gggattatta cgcacacccg caatcatcgc cgtcatcaac
    24841 tggttgataa gttcactggc ggcggggaag ctatccacgc ggatgcggct tttggtttgt
    24901 tgatcgaaaa tgatgtgata caggtcatcg ccatcgtgcc agatgcggaa ctccgcgcgc
    24961 atccggtaat gactgaccgg cgagcgaaac acttccggaa ccaggtcaga aaacggtgcc
    25021 atcatacttt gcaaacgtac cactttttcg gctaactgcg cttcatactg ttctgttgga
    25081 aggtgttcgg gggtcatgat gtatcctgaa aaattaaagt acgcggcgat tgtagggatt
    25141 gctcatcaga tgtccagatc ttgatgaatt cctatttgtg agctacgtct ggacagtaac
    25201 ttgttacaac ctgtagcatc cacttgccgg tcctgtgagt taatagggaa tccagtgcga
    25261 atctggagct gacgcgcagc ggtaaggaaa ggtgcgatga ttgcgttatg cggacactgc
    25321 catccggtgg gaagtcatca tctcttagta tcttagatac ccctccaagc ccgaagacct
    25381 gccggccaac gtcgcatctg gttctcatca tcgcgtaata ttgatgaaac ctgcggcatc
    25441 cttcttctat tgtggatgct ttacaatgat taaaaaagct tcgctgctga cggcgtgttc
    25501 tgtcacagcc ttttccgctt gggcacagga taccagcccg gatactctcg tcgttactgc
    25561 taaccgtttt gaacagccgc gcagcactgt gcttgcacca accaccgttg tgacccgtca
    25621 ggatatcgac cgctggcagt cgacctcggt caatgatgtg ctgcgccgtc ttccgggcgt
    25681 cgatatcacc caaaacggcg gttcaggtca gctctcatct atttttattc gcggtacaaa
    25741 tgccagtcat gtgttggtgt taattgatgg cgtacgcctg aatctggcgg gggtgagtgg
    25801 ttctgccgac cttagccagt tccctattgc gcttgtccag cgtgttgaat atatccgtgg
    25861 gccacgctcc gccgtttatg gttccgatgc aataggcggg gtggtgaata tcatcacgac
    25921 gcgcgatgaa cccggaacgg aaatttcagc agggtgggga agcaatagtt atcagaacta
    25981 tgatgtctct acgcagcaac aactggggga taagacacgg gtaacgctgt tgggcgatta
    26041 tgcccatact catggttatg atgttgttgc ctatggtaat accggaacgc aagcgcagac
    26101 agataacgat ggttttttaa gtaaaacgct ttatggcgcg ctggagcata actttactga
    26161 tgcctggagc ggctttgtgc gcggctatgg ctatgataac cgtaccaatt atgacgcgta
    26221 ttattctccc ggttcaccgt tgctcgatac ccgtaaactc tatagccaaa gttgggacgc
    26281 cgggctgcgc tataacggcg aactgattaa atcacaactc attaccagct atagccatag
    26341 caaagattac aactacgatc cccattatgg tcgttatgat tcgtcggcga cgctcgatga
    26401 gatgaagcaa tacaccgtcc agtgggcaaa caatgtcatc gttggtcacg gtagtattgg
    26461 tgcgggtgtc gactggcaga aacagactac gacgccgggt acaggttatg ttgagaatgg
    26521 atatgatcaa cgtaataccg gcatctatct gaccgggctg caacaagtcg gcgattttac
    26581 ctttgaaggc gcagcacgca gtgacgataa ctcacagttt ggtcgtcatg gaacctggca
    26641 aaccagcgcc ggttgggaat tcatcgaagg ttatcgcttc attgcttcct acgggacatc
    26701 ttataaggca ccaaatctgg ggcaactgta tggcttctac ggaaatccga atctggaccc
    26761 ggagaaaagc aaacagtggg aaggcgcgtt tgaaggctta accgctgggg tgaactggcg
    26821 tatttccgga tatcgtaacg atgtcagtga cttgatcgat tatgatgatc acaccctgaa
    26881 atattacaac gaagggaaag cgcggattaa gggcgtcgag gcgaccgcca attttgatac
    26941 cggaccactg acgcatactg tgagttatga ttatgtcgat gcgcgcaatg cgattaccga
    27001 cacgccgttg ttacgccgtg ctaaacagca ggtgaaatac cagctcgact ggcagttgta
    27061 tgacttcgac tggggtatta cttatcagta tttaggcact cgctatgata aggattactc
    27121 atcttatcct tatcaaaccg ttaaaatggg cggtgtgagc ttgtgggatc ttgcggttgc
    27181 gtatccggtc acctctcatc tgacagttcg tggtaaaata gccaacctgt tcgacaaaga
    27241 ttatgagaca gtctatggct accaaactgc aggacgggaa tacaccttgt ctggcagcta
    27301 caccttctga accacgtccc accgtgctgg tgtttgactc cggcgtcggt gggttgtcgg
    27361 tctatgacga gatccggcat ctcttaccgg atctccatta catttatgct ttcgataacg
    27421 tcgcttttcc gtatggcgaa aaaagcgaag agtttattgt tgagcgagtg gtggcaattg
    27481 tcaccgcagt gcaagaacgt tatccccttg cgctggctgt ggtcgcttgc aacaccgcca
    27541 gtaccgtttc acttcctgca ttacgcgaaa agttcgactt cccggttgtt ggtgtcgtgc
    27601 cggcgattaa acctgctgca cgtctgacgg caaatggcat tgtcggatta ctggcaaccc
    27661 gcggaactgt taaacgttct tatactcatg agctgatcgc gcgtttcgct aatgaatgcc
    27721 agatagaaat gctgggctcg gcagagatgg ttgagttggc tgaagcgaag cttcatggcg
    27781 aagatgtttc tctggatgca ctaaaacgta tcctacgccc gtggttaaga atgaaagagc
    27841 cgccagatac cgttgtattg ggttgcaccc atttccctct actacaagaa gaactcttac
    27901 aggtgctgcc agagggaacc cggctggtgg attctggcgc agcgattgct cgcagaacgg
    27961 cctggctgtt agaacatgaa gctccggatg caaaatctgc cgatgcgaat attgcctttt
    28021 gtatggcaat gacgccagaa gctgaacaat tattgcccgt tttacagcgt tacggcttcg
    28081 aaacgctcga aaaactggca gttttaggct gatttggttg aatgttgcgc ggtcagaaaa
    28141 ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc accactgaca
    28201 cggaacaacg gcaaacacgt cgccgggtca gcggggttct cctgagaatc tcaacagaga
    28261 aaagcaaaaa atgcttgact ctgtagcggg aaagcgtatt atgcacaccc cgcg
//
LOCUS       DAZFWL020000052        27941 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFWL020000052 DAZFWL020000000
VERSION     DAZFWL020000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.052"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..461
                     /locus_tag="ACHKNP_RS11960"
                     /old_locus_tag="pgaptmp_002392"
     CDS             <1..461
                     /locus_tag="ACHKNP_RS11960"
                     /old_locus_tag="pgaptmp_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4704545.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRG
                     QAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
     gene            830..3676
                     /locus_tag="ACHKNP_RS11965"
                     /old_locus_tag="pgaptmp_002393"
     CDS             830..3676
                     /locus_tag="ACHKNP_RS11965"
                     /old_locus_tag="pgaptmp_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HGU4704546.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNGGTLTNHDNQIVFGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATVTVINEKGWQAVKSGAMATDTVVNTGAEGGPDAENGDTGQNVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATDVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIHFDNQTTQDAVLSRAVAKGDSPVTFHK
                     LTTTNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATTGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGGYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            4004..4822
                     /locus_tag="ACHKNP_RS11970"
                     /old_locus_tag="pgaptmp_002394"
     CDS             4004..4822
                     /locus_tag="ACHKNP_RS11970"
                     /old_locus_tag="pgaptmp_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4704547.1"
                     /translation="MRLASRFGHVNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSERY
                     AYIPTITVLESLQREGFQPFFAYQIRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPSRQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRTIHSIDTDIKLNRALWVMAETLLESMR"
     gene            4914..5399
                     /locus_tag="ACHKNP_RS11975"
                     /old_locus_tag="pgaptmp_002395"
     CDS             4914..5399
                     /locus_tag="ACHKNP_RS11975"
                     /old_locus_tag="pgaptmp_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4704548.1"
                     /translation="MTTVTHNSTTPSVSVTAASGNNPPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRMENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            5415..5891
                     /locus_tag="ACHKNP_RS11980"
                     /old_locus_tag="pgaptmp_002396"
     CDS             5415..5891
                     /locus_tag="ACHKNP_RS11980"
                     /old_locus_tag="pgaptmp_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGU4704549.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            5954..6175
                     /locus_tag="ACHKNP_RS11985"
                     /old_locus_tag="pgaptmp_002397"
     CDS             5954..6175
                     /locus_tag="ACHKNP_RS11985"
                     /old_locus_tag="pgaptmp_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU4704550.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            6249..6656
                     /locus_tag="ACHKNP_RS11990"
                     /old_locus_tag="pgaptmp_002398"
     CDS             6249..6656
                     /locus_tag="ACHKNP_RS11990"
                     /old_locus_tag="pgaptmp_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4704551.1"
                     /translation="MSDTLHGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTITLYAKGLTCEAD
                     TLGSCSFVYMAVYATRFSNQLPKASVHQLTHYF"
     gene            complement(7197..8444)
                     /locus_tag="ACHKNP_RS11995"
                     /old_locus_tag="pgaptmp_002399"
     CDS             complement(7197..8444)
                     /locus_tag="ACHKNP_RS11995"
                     /old_locus_tag="pgaptmp_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HGU4704552.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(8516..9430)
                     /locus_tag="ACHKNP_RS12000"
                     /old_locus_tag="pgaptmp_002400"
     CDS             complement(8516..9430)
                     /locus_tag="ACHKNP_RS12000"
                     /old_locus_tag="pgaptmp_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HGU4704553.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            9646..11079
                     /gene="cscA"
                     /locus_tag="ACHKNP_RS12005"
                     /old_locus_tag="pgaptmp_002401"
     CDS             9646..11079
                     /gene="cscA"
                     /locus_tag="ACHKNP_RS12005"
                     /old_locus_tag="pgaptmp_002401"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HGU4704554.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(11087..12082)
                     /locus_tag="ACHKNP_RS12010"
                     /old_locus_tag="pgaptmp_002402"
     CDS             complement(11087..12082)
                     /locus_tag="ACHKNP_RS12010"
                     /old_locus_tag="pgaptmp_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4704555.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            12325..12543
                     /locus_tag="ACHKNP_RS12015"
                     /old_locus_tag="pgaptmp_002403"
                     /pseudo
     CDS             12325..12543
                     /locus_tag="ACHKNP_RS12015"
                     /old_locus_tag="pgaptmp_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            12561..13889
                     /gene="dsdA"
                     /locus_tag="ACHKNP_RS12020"
                     /old_locus_tag="pgaptmp_002404"
     CDS             12561..13889
                     /gene="dsdA"
                     /locus_tag="ACHKNP_RS12020"
                     /old_locus_tag="pgaptmp_002404"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HGU4704556.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKMTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(13997..15535)
                     /gene="emrY"
                     /locus_tag="ACHKNP_RS12025"
                     /old_locus_tag="pgaptmp_002405"
     CDS             complement(13997..15535)
                     /gene="emrY"
                     /locus_tag="ACHKNP_RS12025"
                     /old_locus_tag="pgaptmp_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HGU4704557.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(15535..16698)
                     /gene="emrK"
                     /locus_tag="ACHKNP_RS12030"
                     /old_locus_tag="pgaptmp_002406"
     CDS             complement(15535..16698)
                     /gene="emrK"
                     /locus_tag="ACHKNP_RS12030"
                     /old_locus_tag="pgaptmp_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HGU4704558.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            17114..17728
                     /gene="evgA"
                     /locus_tag="ACHKNP_RS12035"
                     /old_locus_tag="pgaptmp_002407"
     CDS             17114..17728
                     /gene="evgA"
                     /locus_tag="ACHKNP_RS12035"
                     /old_locus_tag="pgaptmp_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HGU4704559.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            17733..21326
                     /gene="evgS"
                     /locus_tag="ACHKNP_RS12040"
                     /old_locus_tag="pgaptmp_002408"
     CDS             17733..21326
                     /gene="evgS"
                     /locus_tag="ACHKNP_RS12040"
                     /old_locus_tag="pgaptmp_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HGU4704560.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(21382..22527)
                     /gene="yfdE"
                     /locus_tag="ACHKNP_RS12045"
                     /old_locus_tag="pgaptmp_002409"
     CDS             complement(21382..22527)
                     /gene="yfdE"
                     /locus_tag="ACHKNP_RS12045"
                     /old_locus_tag="pgaptmp_002409"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HGU4704561.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(22601..23545)
                     /gene="yfdV"
                     /locus_tag="ACHKNP_RS12050"
                     /old_locus_tag="pgaptmp_002410"
     CDS             complement(22601..23545)
                     /gene="yfdV"
                     /locus_tag="ACHKNP_RS12050"
                     /old_locus_tag="pgaptmp_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HGU4704562.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATLLVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(23615..25309)
                     /gene="oxc"
                     /locus_tag="ACHKNP_RS12055"
                     /old_locus_tag="pgaptmp_002411"
     CDS             complement(23615..25309)
                     /gene="oxc"
                     /locus_tag="ACHKNP_RS12055"
                     /old_locus_tag="pgaptmp_002411"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HGU4704563.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(25363..26613)
                     /gene="frc"
                     /locus_tag="ACHKNP_RS12060"
                     /old_locus_tag="pgaptmp_002412"
     CDS             complement(25363..26613)
                     /gene="frc"
                     /locus_tag="ACHKNP_RS12060"
                     /old_locus_tag="pgaptmp_002412"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HGU4704564.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(27126..27758)
                     /locus_tag="ACHKNP_RS12065"
                     /old_locus_tag="pgaptmp_002413"
     CDS             complement(27126..27758)
                     /locus_tag="ACHKNP_RS12065"
                     /old_locus_tag="pgaptmp_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HGU4704565.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNASVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         7678 a         6766 c         6429 g         7068 t
ORIGIN      
        1 acatcctgca ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacgg
       61 agccctgcca tgaatgggat atggccggta ttcagccttc tcccgcacag gcacagaata
      121 ttcgcgaaaa aacggaggcg gtattccgtc tgttccggcc cgtacatccg gttgtggccg
      181 tatcggcccg caccggctgg gaactggata cgctggtcag tgcgctcatg acagcgcttc
      241 ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgagctgcgc acggaatctg
      301 tccggggtca ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt gacacggcgg
      361 agagcgtctg tattgcctct gttgcacgca cggttctgcg ggccgtccgt gactcggtgg
      421 tctctgttgc ccgcgcggta tggaactgga ttttcttctg aacactctgc caggggaaat
      481 gcctgattac attgcgttaa ccaactgaaa acatgccccc ccctcatacc cacctctttc
      541 tcttgcgtga cttccctgct gttaatagaa taaaacgatc gataaaacag gtatcgatcg
      601 ttactatcga tcgtttatag tggttaatgg tctgcgaaaa ggcagctgaa tctcttcatc
      661 atgcagaacg gaattgcaca caacggactg atacttctct ggcggatgag agaggggaaa
      721 catttatgtc cgggaaaaac cactttatct gaccatgctg ttttgtacct gccggtatcc
      781 acttttgtgg gtaccggctt ttttattcac cctctgtaag gaaaagctga tgaaacgaca
      841 tctgaacacc agctacaggc tggtatggaa tcacattacg ggcaccctgg tggtggcctc
      901 cgaactggcc cgctcacggg gaaaacgcgc cggtgtggcg gttgcgctgt ctcttgctgc
      961 tgtcacatca gtcccggcac tggctgctga cacggttgta caggcgggag aaaccgtgaa
     1021 cggcggaaca ctgacaaatc atgacaacca gattgtcttc ggtacggcca acggaatgac
     1081 catcagtacc ggtctggagt atgggccgga taacgaggcc aataccggcg gacaatggat
     1141 acaaaatggc ggtatcgcca acaacactac tgtcaccggt ggtggtcttc agagagtgaa
     1201 tgccggagga agcgtttcag acacggttat cagtgccgga ggcggacaga gccttcaggg
     1261 gcaggcagtg aacaccactc tgaacggcgg tgagcagtgg gtacatgaag gcgggattgc
     1321 aacggttacc gtcattaatg agaagggctg gcaggccgtc aaatccggcg caatggcaac
     1381 cgacacggtt gtgaataccg gcgcggaagg gggaccggat gcagaaaatg gtgataccgg
     1441 gcagaacgtc tacggagatg ccgtacgcac caccatcaat aaaaatggtc gtcagattgt
     1501 ggctgctgaa ggaacggcaa ataccactgt ggtttatgcc ggcggcgacc agactgtaca
     1561 tggtcacgca ctggatacca cgctgaatgg ggggtaccag tatgtgcaca acggcggtac
     1621 ggcgtctggc actgttgtaa acagtgacgg ctggcagatt atcaaggaag gtggtctggc
     1681 ggatttcacc accgttaacc agaaaggcaa actgcaggtg aacgccggtg gtaccgccac
     1741 acatgtcacc ctgaaacagg gtggagcact ggtcaccagt actgcggcaa cggttcttgg
     1801 cagcaaccgt ctgggcaatt tcacggtgga aaacggtaag gctgacggcg ttgttctgga
     1861 gtccggcggt cgcctggatg tactggaagg ccattcagcg cagaaaacac gggtggatga
     1921 cggcggtacc ctggcagtgt ctgccggtgg taaggcaaca gatgtcacca tgacatccgg
     1981 tggtgccctg atagcagaca gtggtgccac tgttgagggg accaatgcca gcggtaagtt
     2041 cagtattgat ggcatatccg gtcaggccag cggcctgcta ctggaaaatg gcggcagctt
     2101 tacggttaat gccgggggac aggcaggcaa caccactgtc ggacatcgtg gaacactgac
     2161 gctggctgcc gggggaagtc tgagtggcag aacacagctc agtaaaggcg ccagtatggt
     2221 actgaatggt gatgtggtca gtaccggcga tattgttaac gcaggagaga ttcactttga
     2281 taatcagacg acacaggatg ccgtgctgag ccgtgctgtt gcaaaaggcg actccccggt
     2341 aacgttccat aaactgacca ccaccaacct cactggtcag ggcggcacca tcaatatgcg
     2401 tgttcgcctt gatggcagta atacctcaga ccagctggtg attaatggtg gtcaggcaac
     2461 cggcaaaacc tggcttgcgt tcacaaatgt cggaaacagt aacctcgggg tggcaaccac
     2521 cggacagggt atccgggttg tggatgcaca gaatggtgcc accacagaag aaggtgcgtt
     2581 tgccctgagc cgcccgcttc aggccggcgc ctttaactac accctgaacc gtgacagcga
     2641 tgaagactgg tacctgcgca gtgaaaatgc ttatcgtgct gaagtccccc tgtatgcctc
     2701 catgctgaca caggcaatgg actatgaccg gattctggct ggctcccgca gccatcagac
     2761 cggtgtaaac ggtgaaaata acagcgtccg tctcagcatt cagggcggtc atctcgggca
     2821 cgataacaac ggcggtattg cccgtggggc cacgccggaa agcagcggca gctatggctt
     2881 cgtccgtctg gagggtgacc tgctcagaac agaggttgcc ggtatgtctc tgacgacagg
     2941 ggggtatggt gctgcaggcc attcttccgt tgatgttaag gatgatgacg gctcccgcgc
     3001 cggcacggtc cgggatgatg ccggcagtct gggcggatac ctgaatctgg tacacacatc
     3061 ctccggcctg tgggctgaca ttgtggcaca gggaacccgc cacagcatga aagcatcatc
     3121 ggacaataac gacttccgcg cccggggctg gggctggctg ggctcactgg aaaccggtct
     3181 gcccttcagt atcactgaca atctgatgct ggagccacaa ctgcagtaca cctggcaggg
     3241 actctccctg gatgacggcc aggataacgc cggttatgtg aagttcgggc atggcagtgc
     3301 acaacatgtg cgtgccggct tccgtctggg cagccacaac gatatgaact ttggtaaagg
     3361 cacctcatcc cgtgacaccc tgcgcgacag tgccaaacac agtgtgcgtg aattgccggt
     3421 gaactggtgg gtacagcctt ctgttatccg caccttcagc tcccggggag acatgagcat
     3481 gggtacagcc gcagccggca gtaacatgac gttctcaccg tcccggaatg gcacgtcact
     3541 ggacctgcag gccggactgg aagcccgtgt ccgggaaaat atcaccctgg gcgttcaggc
     3601 cggttatgcc cacagtgtca gcggcagcag cgctgaaggt tataacggtc aggccacact
     3661 gaacgtgact ttctgataat caggtcactt cccttcaggc cccggtcatt atgaccgggg
     3721 ccttttactt tcagggatca acagatatgc cgggttgtgc atcacgttta ttgcccgaag
     3781 acccttttac tgccaaatgg catctgaatt gatcacagtc tggccagtta ttacccgatc
     3841 ccgatgggga tattgggtga tctccttcca tgaaaatacg cagctcacca gtggagggct
     3901 tctcagacaa taaacgtcat tcatttcatc tgtcaggccg cgtcttctcc gggagacgcg
     3961 gctttttcat ttataccgct aactcattca taaggagcaa ataatgcgat tagcaagtcg
     4021 ttttggtcat gtaaaccaga tacgccgtga ccgcccgctg acacatgaag aactgatgca
     4081 ctatgtgccc agtatttttg gggaagaccg gcatacctcc cgcagtgaac gctatgcgta
     4141 cattcccacc atcaccgtac tagaaagcct gcagcgggag ggctttcagc ccttcttcgc
     4201 ctaccagatc cgcgtgcgcg acccgggccg ccggggatac accaaacaca tgctgcgtct
     4261 gcgccgtgac ggggagataa acggacaaca tgtccctgaa atcatcctgc tgaactccca
     4321 tgacggtact tccagctacc agatgctgcc gggttacttc aggttcgtct gccagaacgg
     4381 gtgcgtctgt ggccagtctc tgggggaagt gcgtgttcca caccggggaa atgtggtgga
     4441 gaaagttatc gaaggggctt acgaggtggt gggcgtgttt gaccggatag aggagaagcg
     4501 tgatgccatg cagtcgctgg tcctgccgcc accgtcacgc caggcgctgg cacaggcggc
     4561 actgacttac cgttatggtg acgaacatca gcccgtcacc accgccgaca ttctgacgcc
     4621 acgacgccgg gaggattacg gtaaggacct gtggagtgct tatcagacca tccaggagaa
     4681 tatgctgaaa ggcgggattt ccggtcgcag tgccagagga aaacgtatcc atacccgtac
     4741 cattcacagc atcgacaccg acattaagct caaccgggcg ttgtgggtga tggcagaaac
     4801 actgctggag agcatgcgct gatgcctttt ccctgaaagc gcagtcctga ctcacttccg
     4861 tccctgcctc cggacattct tccattcatt tactttttat aaggaataat ctcatgacaa
     4921 ccgttacgca taattccacc acaccttctg tttccgtaac cgctgcatca gggaataacc
     4981 cacctcagct ggttgctact ctcgtccctg atgaacagcg catcagcttc tggccgcagc
     5041 attttggcct cattccacag tgggtgaccc tggagccccg tgtcttcggc tggatggacc
     5101 gtctgtgcga agactactgc ggtggtatct ggaatctgta caccctgaac aacggcgggg
     5161 catttatggc acccgaaccg gatgacgatg atgacgaaac atgggtactg ttcaatgcca
     5221 tgaacggtaa ccgcgctgaa atgagcccgg aagccgccgg tattgccgcc tgtctgatga
     5281 cgtacagtca tcatgcctgt cgtatggaga attatgccat gacggtccat tattaccggt
     5341 tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc atcgactgaa
     5401 tggagagaag cacaatgcaa caactttcct ttctgcccgg agagatgacg cccggcgagc
     5461 gcagcctcat tcaacgggcc ctgaaaaccc tggaccgcca tcttcatgaa cccggcgtgg
     5521 ccttcacctc cacccgtgca gcacgggaat ggctgattct gaacatggcg ggactggagc
     5581 gtgaagaatt ccgggtgctg tatctgaaca accagaatca gctgattgcc ggtgaaaccc
     5641 tcttcaccgg caccatcaac cgcacggaag tccatccccg ggaagtgatt aaacgcgccc
     5701 tgtaccacaa tgccgctgcc gtggtactgg cacacaatca cccgtccggt gaagtcacac
     5761 ccagtaaggc agaccggctt atcaccgaac gtctggtaca ggcactgggc ctggtggata
     5821 tccgggtgcc ggaccatctg atagtcggtg gcaaccaggt tttctccttt gccgaacatg
     5881 gtctgcttta acccgtcaca accacatcac acctgttttc acttttatct tctgtcttca
     5941 gaggtatccc attatgaaaa ttatcacccg tggtgaagcc atgcgtattc accaacaaca
     6001 tccgacatcc cgtctttttc cgttctgtac cggtaaatac cgctggcacg gcagtgctga
     6061 agcgtatacc ggtcgtgaag tgcaggatat tcccggtgtg ctggccgtgt ttgctgaacg
     6121 ccgtaaggac agttttggtc cgtatgtccg gctgatgagc gtcaccctga actgagtggg
     6181 aattctgatg agcagaatta tcgccactac cggaccattc ttagccgatt ttctgtaagg
     6241 attttatcgt gtcagacaca ctccacggga caacacttcc cgacgacaat cacgaccgcc
     6301 cctggtgggg gctgccctgc accgtgacgc cctgtttcgg ggcacgtctg gtgcaggagg
     6361 gtaaccggtt gcattacctt gccgaccgcg ccggtatcag aggccggttc agcgatgcgg
     6421 atgcatacca tctggaccag gcctttccac tgctgatgaa acaactggaa ctcatgctca
     6481 ccagcggtga actgaatccc cgccatcagc ataccatcac gctgtatgca aaagggctga
     6541 cctgtgaagc tgatactctc ggcagttgta gtttcgttta tatggctgtt tatgctaccc
     6601 gattctcaaa tcaattaccg aaagcttctg ttcatcaact aactcattac ttttaatgat
     6661 ggggcccttt ctggggccca agaaattaac aacgctaaca aacaacataa ataacaataa
     6721 attaaataaa aaatttcatt cctgcagggg acaccagata cccttcaaac gaaatctacc
     6781 ttcaccccgt aaaagatagg tttggcagca cacttgcctt atatctactc atttttactg
     6841 caacaggttg aaatctcagc actgtcagaa agcgctgatg actaaacagc cctgggccgg
     6901 gcgatgtaac catcacacag aatcctgata gcgaaatatg gcgtgactcg atacttcact
     6961 ccgcaatgca ttccttgatg aattcgcagg accgtgatac acgggacagg tcgctgaatg
     7021 acgacaatgt cctggaaatc agcgaaccgc gcatctgaag tacatttgag cgactgtacc
     7081 agaacatgaa tgaggcgttt ggattaggtg attattagca gggctaagca ctttagtatt
     7141 attattttcc ggttgaggga tagggagata tcgacaacaa ccggaaaagt ttacgtctat
     7201 attgctgaag gtacaggcgt ttccataact atttgctcgc gttttttact caggaagaaa
     7261 atgccaaata gcaacatcag gcagacaata cccgaaattg cgaagaaaac tgtctggtag
     7321 cctgcgtggt caaagagtat cccagtcggc gttgaaagca gcacaatccc aagcgaactg
     7381 gcaatttgaa aaccaatcag aaagatcgtc gacgacaggc gcttatcaaa atttgccacg
     7441 ctgtatttga agacggatat gacacaaagt ggaacctcaa tagcatgtaa cagcttcact
     7501 aatgaaataa tccaggggtt aacgaacagc gcgcaggaaa ggatacgcaa cgccataatc
     7561 acaacaccga taagtaatgc attttttggc cctacccgat tcacaaagaa aggaataatc
     7621 gccatgcata gcgcttcgag taccacctgg aatgagttga gataaccata caggcgcgtt
     7681 cctacatcgt gtgattcgaa taaacctgca taaaagacag gaaaaagttg ttgatcaaaa
     7741 atgttataga aagaccacgt ccccacaata aatatgacga aaacccagaa gtttcgatcc
     7801 ttgaaaactg cgataaaatc ctcttttttt acccctcccg catccgccgc tacgcactgg
     7861 tgatccttat ctttaaaaca catgttgatc atcataaata cagcgccaaa tagcgagacc
     7921 aaccagaagt tgatatgggg actgatacta aaaaatatgc cggcaaagaa cgcgccaata
     7981 gcatagccaa aagatcccca ggcgcgcgct gttccatatt cgaaatgaaa atttcgcgcc
     8041 attttttcgg tgaagctgtc aagcaaaccg catcccgcca gataccccag gccaaaaaag
     8101 agcgccccca gaattagacc tacagaaaaa ttgctttgca gtaacggttc ataaacgtaa
     8161 atcataaacg gtccggtcaa gaccaggatg aaactcatac accagatgag cggtttcttc
     8221 agaccgagtt tatcctgaac gatgccgtag aacatcataa atagaatgct ggtaaactgg
     8281 ttgaccgaat aaagtgtacc taattccgtc cctgtcaacc ctagatgtcc tttcagccaa
     8341 atagcgtata acgaccacca cagcgaccag gaaataaaaa agagaaatga gtaactggat
     8401 gcaaaacgat agtacgcatt tctgaatgga atattcagtg ccataattac ctacctgtcg
     8461 ttaaaaaatt cacgtcctat ttagagataa gagcgacttc gccgtttact tctcactatt
     8521 ccagttcttg tcgacatggc agcgctgtca ttgccccttt cgccgttact gcaagcgctc
     8581 cgcaacgttg agcgagatcg ataattcgtc gcatttctct ctcatctgta gataatcccg
     8641 tagaggacag acctgtgagt aacccggcaa cgaacgcatc tcccgccccc gtgctatcga
     8701 cacaattcac agacattcca gcaaaatggt gaacttgtcc tcgataacag accaccaccc
     8761 cttctgcacc tttagtcacc aacagcatgg cgatctcata ctcttttgcc agggcgcata
     8821 tatcctgatc gttctgtgtt tttccactga taagtcgcca ttcttcttcc gagagcttga
     8881 cgacatccgc cagttgtagc gcctgccgca aacacaagcg gagcaaatgc tcgtcttgcc
     8941 atagatcttc acgaatatta ggatcgaagc tgacaaaacc tccggcatgc cggatcgccg
     9001 tcatcgcagt aaatgcgctg gtacgcgaag gctcggcaga caacgcaatt gaacagagat
     9061 gtaaccattc gccatgtcgc cagcagggca agtctgtcgt ctctaaaaaa agatcggcac
     9121 tggggcggac cataaacgta aatgaacgtt ccccttgatc gttcagatcg acaagcaccg
     9181 tggatgtccg gtgccattca tcttgcttca gatacgtgat atcgactccc tcagttagca
     9241 gcgttctttg cattaacgcc ccaaaaggat catcaccgac ccgacctata aatccactta
     9301 ttccgcctaa tctggcgatt cccaccgcaa cgttagctgg cgcgccgcca ggacaaggca
     9361 gtaggcgccc gtctgattct ggcaagagat ctacgaccgc atcccctaaa acccatactt
     9421 tggctgacat ttttttccct taaattcatc tgagttacgc atagtgataa acctctttct
     9481 cgcaaaatcg tcatggattt actaaaacat gcatattcga tcacaaaacg tcatagttaa
     9541 cgttaacatt tgtgatattc atcgcattta tgaaagtaag ggactatatt tttataaaag
     9601 ttaacgttaa caattcacca aatttgctta accaggatga ttaaaatgac gcaatctcga
     9661 ttgcatgcgg cgcaaaacgc actagcaaaa cttcatgagc accggggtaa cactttctat
     9721 ccccattttc acctcgcgcc acctgccgga tggatgaacg atccaaacgg cctgatctgg
     9781 tttaacgatc gttatcacgc gttttatcaa catcacccga tgagcgaaca ctggggacca
     9841 atgcactggg gacatgccac cagcgacgat atgatccact ggcagcatga gcctattgcg
     9901 ctagcgccag gagacgataa tgacaaagac gggtgttttt caggtagtgc tgtcgatgac
     9961 aatggtgtcc tctcacttat ctacaccgga cacgtctggc tcgatggtgc aggtaatgac
    10021 gatgcaattc gcgaagtaca atgtctggct accagtcggg atggtattca tttcgagaaa
    10081 cagggtgtga tcctcactcc accagaagga atcatgcact tccgcgatcc taaagtgtgg
    10141 cgtgaagccg acacatggtg gatggtagtc ggggcgaaag atccaggcaa cacggggcag
    10201 atcctgcttt atcgcggcag ttcgttgcgt gaatggacct tcgatcgcgt actggcccac
    10261 gctgatgcgg gtgaaagcta tatgtgggaa tgtccggact ttttcagcct tggcgatcag
    10321 cattatctga tgttttcccc gcagggaatg aatgccgagg gatacagtta ccgaaatcgc
    10381 tttcaaagtg gcgtaatacc cggaatgtgg tcgccaggac gactttttgc acaatccggg
    10441 cattttactg aacttgataa cgggcatgac ttttatgcac cacaaagctt tttagcgaag
    10501 gatggtcggc gtattgttat cggctggatg gatatgtggg aatcgccaat gccctcaaaa
    10561 cgtgaaggat gggcaggctg catgacgctg gcgcgcgagc tatcagagag caatggcaaa
    10621 cttctacaac gcccggtaca cgaagctgag tcgttacgcc agcagcatca atctgtctct
    10681 ccccgcacaa tcagcaataa atatgttttg caggaaaacg cgcaagcagt tgagattcag
    10741 ttgcagtggg cgctgaagaa cagtgatgcc gaacattacg gattacagct cggcactgga
    10801 atgcggctgt atattgataa ccaatctgag cgacttgttt tgtggcggta ttacccacac
    10861 gagaatttag acggctaccg tagtattccc ctcccgcagc gtgacacgct cgccctaagg
    10921 atatttatcg atacatcatc cgtggaagta tttattaacg acggggaagc ggtgatgagt
    10981 agtcgaatct atccgcagcc agaagaacgg gaactgtcgc tttatgcctc ccacggagtg
    11041 gctgtgctgc aacatggagc actctggcta ctgggttaac ataatatcag gtggaacaac
    11101 ggatcaacag cgggcaaggg atccgcgtca ctcttccccc ttcacgacct tcaataatat
    11161 gcaatgcagc ttcccgcccg ataatgtcat gtggaagctg aattgtggtc agcggcggta
    11221 aaaacagatg cccgacgcca accagattat caaagcccat tacggcgaca tcctgcggga
    11281 ttcgtacccc cttcgccaga agaacctgat aagccacaaa ggctgcgcga tcgttaccac
    11341 atatcagaac atcaaaatct ggtttgcccg atttgaagtg gtcattgagt aaacttgcga
    11401 gatcggtgta gtgatcatca cctgttgcca tgtgaaattg tttcacctca gccagatctc
    11461 gtccagcatc acgccaggcc tgctcaaatc cctgccgacg ataccctgtt gccaacgcac
    11521 tttccggtag ccagaagcat aacggttgac gatagcccgc cgcgagcaaa tgctgtgttg
    11581 attcatattg tgcagtgtaa tcatcaggga tataactggg taacgctggg tcatccgcca
    11641 cacagttcgc caatacaata ttttcaccat acagagactc aggcagcgtg atatgtcgca
    11701 gccccattgt agtatagata atgccatccg gacggtgggc aagcagctga cgtgccgcgc
    11761 gggcagcgtc atcttcagaa aaaatattga ttaaaaaact attccagccg aactcgctgg
    11821 cggtttgctc aatggcaagc agaatatcaa cagagaaagg agtggtagcc gtgtcctgcg
    11881 ccagcacggc gagagtcgac ggcttacgtc cttgagcgcg catcttacgg gcggaaagat
    11941 caggaacata attcagggtc tggattgcct gcaatacgcg gtcacgcgtt gcaggacgca
    12001 cagattctgc attatgcatc acccgggaga ctgtcatcat cgacactccc gctaggcgtg
    12061 cgacatcctt taatgaagcc atacccaagc cgtttgccgt aaaacgggca ctgtagcaga
    12121 aacagacgtc actggcgaga tccaacgata ttctcaacgc cctatcaccc gacacggcaa
    12181 tacaataaaa aataacaata attgccggat aattgtactc aattccgcct ctgttctcgc
    12241 caacgagtaa ccatataggc aagtaagtaa aacatgccaa ctggcatcgc caacctggag
    12301 aagaaaatat aaatatggat cattgtgctg ccgctgtatc ccgacatcag cccggaaatt
    12361 attgcgattg ctatcggttc aggtgcaatt ggctgcacga tcgttacgga ctcgcttttc
    12421 tggctagtga agcaatattg cggcgctacg ctcaatgaaa catttaaata ctatacgaca
    12481 gcgacattta tcgcttcagt cgtcgctctg gcgggcacat tcctgctgtc atttatcatc
    12541 taagcgcaaa gagacgtact atggaaaacg ctaaaatgaa ctcgctcatc gcccagtatc
    12601 cgttggtaaa ggatctggtt gctcttaaag aaaccacctg gtttaatcct ggcacgacct
    12661 cattggctga aggtttacct tatgttggcc tgaccgaaca ggatgttcag gacgcccatg
    12721 cgcgcttatc ccgttttgca ccctatctgg caaaagcatt tcctgaaact gctgccactg
    12781 gggggattat tgaatcagaa ctggttgcca ttccagctat gcaaaaacgg ctggaaaaag
    12841 aatatcagca accgatcagc gggcaactgt tactgaaaaa agatagccat ttgcccattt
    12901 ccggctccat aaaagcacgc ggcgggattt atgaagtcct ggcacacgca gaaaaactgg
    12961 ctctggaagc ggggttgctg acgcttgatg atgactacag caaactgctt tctccggagt
    13021 ttaaacagtt ctttagccaa tacagcattg ctgtgggctc aaccggaaat ctggggttat
    13081 caatcggcat tatgagcgcc cgcattggct ttaagatgac agttcatatg tctgctgatg
    13141 cccgggcatg gaaaaaagcg aaactgcgca gccatggcgt tacggtcgtg gaatatgagc
    13201 aagattatgg tgttgccgtc gaggaaggac gtaaagcagc gcagtctgac ccgaactgtt
    13261 tctttattga tgacgaaaat tcccgcacgt tgttccttgg gtattccgtc gctggccagc
    13321 gtcttaaagc gcaatttgcc cagcaaggtc gtatcgtcga tgctgataac cctctgtttg
    13381 tctatctgcc gtgtggtgtt ggcggtggtc ctggtggcgt cgcattcgga cttaaactgg
    13441 cgtttggcga tcatgttcac tgcttttttg ccgaaccaac gcactcccct tgtatgttgt
    13501 taggcgtcca tacaggatta cacgatcaga tttctgttca ggatattggt atcgacaacc
    13561 ttaccgcagc ggatggcctt gcagttggtc gcgcatcagg ctttgtcggg cgggcaatgg
    13621 agcgtctgct ggatggcttc tataccctta gcgatcaaac catgtatgac atgcttggct
    13681 ggctggcgca ggaagaaggt attcgtcttg aaccttcagc actggcgggt atggccggac
    13741 ctcagcgcgt gtgtgcatca gtaagttacc aacagatgca cggtttcagc gcagaacaat
    13801 tgcgtaatgc cactcatctg gtgtgggcga cgggaggtgg aatggtgccg gaagaagaga
    13861 tgaatcaata tctggcaaaa ggccgttaat aacgtttcaa cgcagcatcg caatcctttc
    13921 cctgggtgag cgatgctgcc gatggcgcag acttaagatc cccgctctta cccgctataa
    13981 ccccctcctt ttatcatcac ccaacgcctt tcgctgtaaa cggcggtttc gcaaaccaaa
    14041 ccaaaaccgt aagtaagata aaagcaatag ccgccatacg gaaaatctca tttgcagaaa
    14101 tagaaagtga ctgctgggtt atttcattat taatttcatt aagaactcct gaaagcgaac
    14161 catagtattt atccataatt tgtgacgatg aattaaacac ggggttaaat tgatcgatgg
    14221 ttgctgtcaa ctgactatgg tgtaacgatt cgcgtcgtcc ccacagcgtc attgtcaacg
    14281 acgtaccaac tgatcctgac aaggtacgaa aaaaattact catactcgag gcattggcaa
    14341 atttattatc tggcaagcct gaaaacgaaa tcgttgttaa gggtaaaaag aaacaggcaa
    14401 cggcgaatcc ctggaaaaac tgtggcaaaa tgatgcctgt aaaatcaatc gttggcataa
    14461 atgtcacaga acgccagtaa tagcaaaccg catacatcaa aaaactaaat gtcactaaca
    14521 accgcatgtc tattttgttg ccataacgtc ctatcaaagg tgaaattaat agtggcatga
    14581 tgccgatggg cgcataagca agtccggccc atatcgcatt ataccccatc gtttcctgga
    14641 gtaactgcgg cataaggacg atcgctccag agtaaaataa atacgcgcat gtgatactca
    14701 caataccaat ggtgaagtta cgggacttaa acaaactgag atcaagaatc gggttctctg
    14761 aggtcgactc ccaaatgact aaagagatca gagaaataac tgatactact gttaatatta
    14821 ttattgtact cgagttgaac caatccagat cgcgcccttt atcaagcata atttgcaagc
    14881 caccaacacc gagcactaac agggtcagtc ctggtagatt cattttgacc ggtgaagttt
    14941 cagtttctct tcctttaagt aaggttaagc ataatgtcag gacgataatc cccataggga
    15001 cattgattaa aaatatccaa ccccagctaa agttatcgca aatataaccg cccaatatcg
    15061 gcccacatat cggagcgata atcacggtca ttgaccataa tgccagagca aatgttcttt
    15121 tttctggtgg ataattcctt aataataaac tctgtgacag tggaattaac ggccccgcca
    15181 ttaatccctg aacgactcta aaaaatatca gcacatcaag attggtcgat aaactacaca
    15241 ttaaggaaga cagcgaaaaa aaagtgactg aaagtaaaaa taaccttaat tcgcctattc
    15301 tttgtgccag cctgccggta acagggatcg caatggcatt tgctacgcca aacgaggtga
    15361 taacccaggt gccttcgtct gttgatgctc ccagaaagcc agatattgtc ggtattgcga
    15421 cgttagaaat agtggaatcc aacatttgca taaacgtcgc taatgacaat gcaatagtaa
    15481 cgcaccataa cgtcccaccg gttaatggtg ccggagtcga tttagtgatt gccattaaag
    15541 ttgtccatta tgcgaaataa tgttgctaat ttctttttct atcggactgg tatcgataac
    15601 taaagcctta ctggtataag ccggcatgga cgtcacggtt gaagccagat caggcatctc
    15661 agcaatgtct tcgttcttcg tatcaatagt tgcagtcatc gataaaccaa tgcgtaaggg
    15721 gtgttccatg agttcttttg gatcaagaga aacttcaacc ggtacacgct gaacgatttt
    15781 gatccagttc cctgtcgcat tttgtgcagg taataaggag aacgcattgc cggttcccat
    15841 attgatccct gtcacccgac catgaaacac aacattttca ccataaagat cgctgataat
    15901 attgactgat tgaccaatcc gtacatccgt gagttgtgtt tctttaaagt tggcattaac
    15961 ccacatttga cgtgccggta cgacagccat taacgattgt ccgggactca ctgtttcgcc
    16021 gacctgaaca ctccgctggg caatatagcc agtaaccgga ctccttatat ccgtacgttt
    16081 aagcgccaac caggcttctt tagttgcatc cgccgcttcc acgacttgtg gctgacggtt
    16141 taatggtgtg ttcattacta aagctttatt cgctttataa gcctggatag cggcattcag
    16201 tgccgctttg ctacttatta acgtatcttt ggtatgctcc agcgtttctt ttgaaataac
    16261 cccctgcttc gctaacggta ctcgacggtt ataatcttct aaagattgtt gatactgaat
    16321 acgtgctgaa gcgacttcgg cactgtattg tttatcctgt aagtacagtt tattcgtttg
    16381 ccgaacaata tttgccagat tatttttagc tttattgagt gcgatagtgg catcagtttt
    16441 atccagtgaa actaaaatgt caccttgtcg aacgtagttc gtatctttat gattaacgac
    16501 agtgacacta cctgagactt gtgcagaaat tggatctgca ttccccgtga cataggcgtc
    16561 atctgtacta atcatgtctt ctaattccat tgaccaatag gcataggcac ctgaaaacgc
    16621 aataaataaa actactgcca ataaagaaaa gtattttctt ctgttagaat gttttttatt
    16681 tgaattaatc tgttccacta ttatctctca tttctcatag atgaaattta tgaattgaat
    16741 gaaaagagca ttcattcttc atatcaaaca cagtgcatgt gttgcacatt atcccaggaa
    16801 cgttggaggc ctaatttaaa tgaggtgcaa aaaaaacaac agaggactaa accgtggctt
    16861 ttgcaataca attcttacgc ctgtaggatt agtgagaaga cttatagtgc taacttgaaa
    16921 ctataaatca tcggtataat ccctgatttt attgttgaca tttcatttat gccgactatt
    16981 tatatggtat acttgtcgaa ttatcttaaa ggaagctcag attttcttat ttttattgag
    17041 aaaatgagat gacgccttat gtctgtatta ctacagggag aagggagatg cttcattgca
    17101 aagggaataa tctatgaacg caataattat tgatgaccat cctcttgcta tcgcagcaat
    17161 tcgtaattta ttgatcaaaa acgatattga aatcttagcg gagttgactg aagggggaag
    17221 cgccgttcag cgggtggaaa cacttaagcc tgatatcgtc atcattgatg tcgatatccc
    17281 cggagttaac ggtatccagg tgttagaaac gctgaggaag cgccaatata gcggaattat
    17341 tattattgtc tccgctaaaa atgaccattt ttacgggaaa cattgtgctg atgctggcgc
    17401 taatggattc gtgagtaaaa aagaaggcat gaacaatatc attgcggcta ttgaagctgc
    17461 aaaaaatggc tactgctatt tccccttctc tctcaaccgg tttgttggaa gtttaacgtc
    17521 cgaccagcaa aaactcgact ccttatcgaa acaagaaatt agtgtcatgc ggtatattct
    17581 tgatggcaag gataataatg acattgctga aaaaatgttc atcagcaaca aaactgtcag
    17641 cacttataaa agtcgcctga tggaaaaatt agaatgtaaa tcactgatgg atctttacac
    17701 attcgcgcaa cgtaacaaaa tcggctaatc acatgaagtt tttaccctat ttttttcttc
    17761 tctgttgtgg tctttggtcg accataagtt tcgcagacga agattacatc gaatatcgtg
    17821 gcatcagtag taacaaccgt gtcacacttg atccagtacg tctgagcaac aaggaattac
    17881 gttggttagc gagcaaaaaa aatcttgtga ttgcagtaca caagtcccaa acggctacct
    17941 tgttgcatac cgattcgcag caacaggttc gtggtattaa tgctgattat ttaaatcttt
    18001 taaaaagagc gttaaatatc aaattaacac tccgggaata cgccgatcat caaaaagcaa
    18061 tggacgcgct tgcagaaggt gaagtcgata tagtgttatc acatttagtt acttcgccgc
    18121 ctcttaatag tgacattgct gcaaccaaac cattgataat tacctttccg gcgctggtaa
    18181 ccacccttca cgactcaatg cgaccgctta cctcaccaaa accagtaaat attgctcggg
    18241 tagcaaatta ccccccagac gaggtaattc atcaatcgtt tccaaaagca acaattatct
    18301 cttttacaaa tttatatcag gcattagcat ccgtatcagc cggacagaat gattacttta
    18361 ttggtagtaa catcattacc agcagtatga tttcccgcta tttcactcac tccttaaatg
    18421 tagtgaaata ttataactcg ccgcgtcaat ataatttttt cttgaccaga aaagaatccg
    18481 tcattcttaa tgaagtactc aatagatttg ttgatgcttt aacaaatgaa gttcgctatg
    18541 aagtttcaca aaattggctt gatacaggaa acctggcctt tctgaacaaa ccattagaac
    18601 tcactgaaca tgaaaaacag tggattaagc aacatcccga tttaaaggtg ctggaaaatc
    18661 cttactctcc tccctattct atgacggatg aaaatggctc ggttcggggc gttatggggg
    18721 atattcttaa tattattacc ttgcaaacag gtttaaattt ttctccgatc accgtttcac
    18781 acaatatcca tgccggaaca cagcttaacc ccggtggatg ggatataata cctggcgcta
    18841 tttatagtga agatcgagaa aataatgttt catttgcaga agctttcata acaacgccat
    18901 acgtttttgt catgcaaaaa gcacctgaca gtgaacaaac attaaaaaaa ggaatgaaag
    18961 ttgccattcc atattattat gagctgcatt cgcaattaaa agagatgtat ccggaggttg
    19021 aatggataca ggtcgataat gccagcgctg catttcacaa ggttaaggaa ggtgaacttg
    19081 atgctctggt cgcgacacag ttaaattcgc gttacatgat cgatcattac tatcctaatg
    19141 aactttatca ttttcttatt cctggcgttc cgaatgcatc gctttcgttc gcttttcctc
    19201 gcggagaacc ggaacttaag gatattatta ataaagcact gaatgcaatt cccccaagcg
    19261 aagttctgcg cctgacggaa aaatggatta aaatgcccaa tgtgaccatt gacacatggg
    19321 acctatatag cgagcaattt tatattgtta cgacattatc cgttttatta gttggcagta
    19381 gccttttatg gggattctac ctgttacgct cagttcgtcg tcgtaaagtc attcagggtg
    19441 atttagaaaa ccaaatatca ttccgaaaag cactctcgga ttccttaccg aatccaactt
    19501 atgttgtaaa ctggcaaggt aatgtcatta gtcataatag tgcttttgaa cattatttca
    19561 ctgcggatta ctacaaaaat gcaatgttac cattagaaaa cagtgactca ccctttaaag
    19621 atgttttttc taatgcgcat gaagtcacag cagaaacgaa agaaaatcga acaatataca
    19681 cacaggtatt tgaaattgat aatggcatcg agaaaagatg cattaatcac tggcatacat
    19741 tatgcaatct tcctgcaagt gacaatgcag tatatatttg tggttggcaa gatattactg
    19801 aaacgcgtga tctaattaat gcactcgagg tagaaaaaaa taaagcgata aaggctaccg
    19861 tagcaaaaag tcagtttctg gcaacgatga gtcacgaaat aagaacacca ataagctcta
    19921 ttatgggctt cctggaactt ctgtcgggtt ctggtcttag caaggagcaa cgggtggagg
    19981 cgatttcact tgcctacgcc accggacaat cactcctcgg cttaattggt gaaatccttg
    20041 atgtcgacaa aattgaatcg ggtaactatc aacttcaacc acaatgggtc gatatcccta
    20101 ctttagtcca gaacacttgt cactctttcg gtgcgattgc tgcaagcaaa tcgatcgcat
    20161 taagttgcag cagtacattt cctgatcatt acctggttaa gatcgaccct caggcgttta
    20221 agcaggtctt atcaaattta ctgagcaatg ctctcaaatt taccaccgag ggggcagtaa
    20281 aaattacgac ctccctggtt cacattgatg acaaccacgc tgttatcaaa atgacgatta
    20341 tggattctgg aagtggatta tcgcaggaag aacaacaaca actctttaaa cgctacagcc
    20401 aaacaagtgc aggtcgccag caaacaggat ctggcttagg cttaatgatc tgcaaagaat
    20461 taattaaaaa tatgcagggc gatttgtcat tagaaagtca tccaggcata ggaacaacat
    20521 ttacgatcac aatcccggta gaaattagcc agcaagtggc gactgtcgag gcaaaagcag
    20581 aacaccccat cacactacct gaaaagttga gcatattaat cgcggatgat catccgacca
    20641 acaggctatt actcaaacgc cagctaaatc tattaggata tgatgttgat gaagccactg
    20701 atggtgtgca agcgctacac aaagtcagta tgcaacatta tgatctgctt attactgacg
    20761 ttaatatgcc gaatatggat ggttttgagt tgactcgcaa actccgtgag caaaattctt
    20821 ccttacccat ctgggggctt acagccaacg cacaggctaa cgaacgtgaa aaagggttaa
    20881 gttgcggcat gaacttatgt ttgttcaaac cgttgaccct ggatgtactg aaaacacatt
    20941 taagtcagtt acaccaggtt gcgcatattg cacctcagta tcgccacctt gatatcgaag
    21001 ccctgaaaaa taatacggcg aacgatctac aactgatgca ggagattctc atgactttcc
    21061 agcatgaaac gcataaagat ctacccgctg cgtttcaagc actagaagct ggcgataaca
    21121 gaactttcca tcagtgtatt catcgcatcc acggtgcggc taacatcctg aatttgcaaa
    21181 agttgattaa tattagccat cagttagaaa taacacctgt ttcagatgac agtaagcctg
    21241 aaattcttca gttgctgaac tctgtaaaag aacacattgc agagctggac caggagattg
    21301 ctgttttctg tcagaaaaat gactaaatag cggctcccac aatgttcaaa tgtgggagct
    21361 atttaccagc acatcttaca tttatgatga gaactcctgg cgaatttgtt ccccatgctg
    21421 gtcgagcgtt gccgctcccg gcataacatg cgggtccgcg cagccgctga tttttatcgg
    21481 attacccggc atcattattc ccccggcttc aatcaacata tttctcgcct gagtttgtgg
    21541 caatttaatg gcctcagcca cacttaataa cggcgcgacg ggtacaccaa cttcatgtat
    21601 tctggctaac caaacttcag ctgcctgcgt ttttaacgtc cgctcaatat attgtttaag
    21661 aatagcctgg ttttgtacgc gtaaaatatt gctgctaaat cggggatcat taaccagttc
    21721 cgtaagctcc agtgcctggc ataacgcaga aaaaagcttg tcattaccac aacaaatcgt
    21781 aatcggctta tcctgagtat tgaaaacatc aaaaggtgcc atgtagggat ggcgatttcc
    21841 cagacgttgt ggtgacttcc ctgtcgcgat atatgccatc agaccatgct ccagaaaact
    21901 cagcgtggca tcaaacatcg ctatatcgac atgcgcccct ctctggctct tttcgcggcc
    21961 ataaagtgca ctcactattc cgctgaataa atagacaccg ccgcatagat ccgcaagaga
    22021 tgtaccaacg cgcactggcg gagcatcagg atatcctgtt tccatcatta tcccgctcat
    22081 tgcctgaatg atggtatcgt aggcaggagc atcttttagc ggaccggtat gtccgaaacc
    22141 tgacgatgaa gcatatatga ggcgcgggtt gatttcttga agcgtttccc atgaaaaccc
    22201 cagtttttcc attgtacctg ggcgaaaatt ctcagctaat acatcagctt gtttgagcat
    22261 atttataaat atacttttat cgtgatcatt ctttaaatca agaaccacac tctctttgcc
    22321 atgattaata aaactgtaat agagtgactg tccatccaca tagggaccaa atgtgcgggt
    22381 atcatcacca tgacccggcg gctcaacttt aattaccctt gcgcccatat tacaaagaag
    22441 ttgagttccg aaaggtccat taaggacatg tgtcatatcg ataactaata agccttcaaa
    22501 cggccctttg ctttcattat ttgtcatttg cttatgcctt gaaaaaagcc gaaggtgaaa
    22561 agacacttcg gcatttatgt aaaatcaata acatgattaa ttatgaaacc agtcgactga
    22621 cataaatcca caagggagcc gtgacgacaa agcccagtac gctcacagcc aatgacgctg
    22681 taccagtacg ggtgtagaca ttaaaccggc tggcaataat gattccggag aatgccggcg
    22741 gtaatgcgcc tgccagtacc atcatctgca gatgttcgct gttcaaatga catgccatac
    22801 caacgagaag cagtgccagt ggcatcagaa tcagcttcag gaaggtgtta taagcaattt
    22861 cggcactgaa ctcgaattta tgtgcagcca gagtcaaccc cgcagcgaat accgctaccc
    22921 ctgagttagc cttcgcaatc agattaaagg ttgggtccca tgctgccgga atttttaccc
    22981 caaccaacac caggagcgtt gccagaacag gtgcccatac tactggctcc tttgccgcag
    23041 aaattaatgc gctcagatta ctattcttct taccatccgc tcctgaggaa ggattcagca
    23101 aatacagacc aataggaata gtaattgcgt taacaataat agaaataatt gctaccacta
    23161 aacctgttga tacggaatca ccataaatag gatcgagaac tgcaaacccc aggaatccaa
    23221 tggtaggtga acccgcaatt aatgcacata cagctgcttc tgcatgggta cgtttaaaaa
    23281 atttgtagca accaaaccag gagaaaaaga aacatccgac aataaccaca agtgatacaa
    23341 gggtcagacg agtgtccgca aaaatcattt ccctgtttgc ccgggtaata gaaacaaata
    23401 gagccgcagg aagagcgtag tttaatacca gtttattaaa tgcgcgggct tgatcttctg
    23461 aaaaagtttc tcgtctgccg ctaaaataac ccaacagcat gattacgata ataggcaata
    23521 aatcgccaat aaaaaatgtt agcataatag tatttccgat aagggtcccc tcaccatcgc
    23581 ataatgagtt ggcaagagga gacgatgtca gtatttaatt accagcgact tgttttgggt
    23641 taagtttggt aatatggcca ctttcagttc ctgctgcagg gtcgatgacc acattaataa
    23701 tggtcggttt gcgcgactga ataccggtgg ttaaagcatg acgaagttca tctgtcgtgg
    23761 tgacgttata gccaacgcca cgaaacgcat ccattaattt gtcatacctt gcatggtgca
    23821 acagatccgt tggtgatggt gcaccagcgc cactgagatc aacaccgtct cctctgtaga
    23881 tgccgccatt attaaaaata acgatcgtca ccggcaggtt atatcgacaa atcgtttcaa
    23941 tttccatccc actgaaacca aaagcactat caccttcaat ggcgacaacc ggagaaccag
    24001 aagtcacgct agcaccgatg gcatagccca taccgatgcc catgacaccc caggtaccac
    24061 aatccagacg acgacgtggt ttatacatat caataatatt tcgtgcatta tccagggtat
    24121 ttgcaccttc attaactaaa taaatatcct ggttctcgcg caatacgtcg cgcacagcac
    24181 ttaatgcatt aaagtaattt aatggttgag tatctgtact taatttttca tgcatttttt
    24241 gtgcattttg ctgcttgtgg atatttaaaa tatcgcgcca taccagtgga gtcgtaaatg
    24301 tgttttgttt cagttctgcc agcatacctt gcatactgga tgcaatatca ccaacgactg
    24361 gcacagcaat ggggcggttg ctgtcaattt cctgcggttc aatatccagt tgaataaact
    24421 gtgtatctgc cgcccatcct tttttaccgt gtgccaataa ccaattcagt cgtgcaccaa
    24481 caagcatgac aacgtcagca tttgccaggg caaacgaacg cgcagctgcc gcagaaagtg
    24541 gatgcgtatc ttcaaggatc cctttcgcca tagacattgg caggaatgga atctgagtac
    24601 tttcaataaa ctcacgaagc tgttcatcag cttgtgaata cgccgcgcct ttgccaagga
    24661 taattaatgg ccgttcagct tttgctaaaa gcgaaattgc gctagtgact gacttcgggc
    24721 atggcaataa tgctggcgac ggattttcaa ctttaacaat cgtggttaac gcttcgtctt
    24781 tttccatcgt cgcggccagg acatttgctg gcaaatcaag ataaactccg ccagggcgac
    24841 ccgatacaga gacccggata gcgcgtgcca atgcaatgcc aagatcctgc ggctgattaa
    24901 cgcgaaatgc tgctttggca tacggttttg ccgcattcat ttggtccagc tcttcataat
    24961 caccttgctg taggtcgacg atcgcgcggt cgctggagcc gctaatcatg atcatcggaa
    25021 aaccatttac cgttgcgttg gccaatgcgg tcaaaccatt gaggaatcct ggcgcagaaa
    25081 ctgtcaggca gatccccggt ttttgggtaa gaaaaccgct tgccgcagcg gcatagcctg
    25141 ccgactgctc atgacgaaaa ccaatataac gaatgccttc cgcctgggca tggcgtgcca
    25201 tatccgtcac aggaatacct acaacaccat aaatagtgtc aatattattc tgttttaatg
    25261 cttcaacgat gatatgcata ccatctgtca tttgaagttg atctgacatg attaataacc
    25321 tcgaaatttt tataaattgg gagccagaag cccccgttaa tatcagatgg cgtggttttg
    25381 cttcattgca gcaatttcat cgtcgctata acccagctcc tgcaatacag cagcggtatg
    25441 ttcacctaat agcggcgcag ctttaatatc cggcgtaaag gcagagaatt tcattggaca
    25501 accaacggtc agatattttc cacgcaacgg ttgttccact tcaacaacac tgccactttg
    25561 gcgcaaagag ggatcaagtg aaatttcttt catacttaaa accggtgcac aaggaatatc
    25621 aaactgagtc aaataggcca ccgcttcatg tttatcaata gtgacagtgt atttttcgat
    25681 ttcagcaaaa atatcgaaaa tatgtggctg tcgtgcatgg gctgtactgt atgccggatc
    25741 ggtaatccat tctggtttgc cgatggcttt acaggtgttt tcccagtttt gctcctgaat
    25801 agtgaaataa atataggcgt taggatcggt ttcccagcct ttacatttca ggatccagcc
    25861 aggctgaccg ccaccacctg cattaccacc gcggggaact gcatcaccaa atgtaccatt
    25921 cggatactgc gggtattctt ccagataacc caatttatcg agacgctgct ggtcacgtaa
    25981 tttcacgcgg caaaggttca atacggcatc ctgcattgac atggtgactc gttgcccacg
    26041 ccccgttttt tcgcgatgca gcaaagcagc aagtaaaccg atcagcaaat gcattccggt
    26101 gttgctgtca cccaacgctg cagcgcttac cagcggcgga ccatcccaaa aacccgtagt
    26161 ggatgccgcg ccacccgctg cctgagcaac gttttcatag gcttttacat tcacataagg
    26221 cgaacactca tcaaaccctt tgatcgaacc aaaaatcaga cgtggattga tttcttgaat
    26281 atgctcccag gtgaagccca tgtgatcaat ggcccctgga tgaaagttct cgactaagat
    26341 atcagcttcg cggatcagct tttccattac ctctttgcct tccgctgttt tggtatttaa
    26401 ctcaatagaa cgtttgttac tgttaagcat ggtgaagtaa agcgcatcga tatcaggaat
    26461 atctcgcagc tggtgacgcg ttacgtcacc aacgccggga cgttcaattt taataacgtc
    26521 agcgccaaac caggccagca tttgagtaca agatgggcca gattgcacac cggtgaaatc
    26581 gagaacttta attccttgaa gtggagttga catagcaata cctattagtt aattaaatat
    26641 gtgttaattt agcgtttatt taaggcgtaa tatcttctat aaataacccg caaaatatcg
    26701 tcgtagctgc actcgatatt tttaaaatcc tgtatcaagt gctgaaaatc atttcatcgc
    26761 cacttccaat tcctttctgc atggtaagta gtgaggacga aaagaagaat atgataaata
    26821 ttgattcgtg gtaatcagag agagtcgcga catttgtgat gttattctta caactgtaag
    26881 aagcgccggt gatttacaag taagggtatg attacgcttt ttaaagcgta ccataaaaca
    26941 taacataata aatgatttgt gatgacacgg gattattggg atttactttg aggaagataa
    27001 aatgcgtgtt tatagaaccg cgagcgccaa ataattttat gaccacaacc tgtcgtattg
    27061 tggtcataaa aaattgtcgg ctggagggag ttaaatcctt tacctcaata tactgcctgt
    27121 cagcattagt tcacaaacag tgcttcgctg tcgacgatga ttccatcttc cgcaccttta
    27181 agcgccaggt ttgcctcgta gtattgcttt ttatcaagca gtttctgcgc atcagcaagt
    27241 gcattacgag tctgtttaag cggcatcaga tattggtttt ccattacgcc aacaccggcc
    27301 aggcgaagtt cttccattgc gcccttttta tcccctttcg ccattttttc attggcaatt
    27361 tttatcgcgg cttctttttc gggagttgct acataactct cagaaattcc gacagatgca
    27421 ttaatgacaa tatattggtc atcattgaga ttggtctttt tacctggctt agcgaacttt
    27481 gcccattcgg tactgtcatc tgacagcaaa gcggaagctt cattggtcag ttcttttgcc
    27541 ttttctggat cgccatgaaa cagagccagg cgcgctacct gcacgtcacg catcgcatat
    27601 aaaccttgtt cagagatacg ctctgctgct gccgttttct gggtttgctg aacttgctga
    27661 gtttgctgtt gtgccgctac cgatgcatta tcagcagccc atacagttga agatgccaga
    27721 attgctgtga ccatcgtagc cataattaaa cgtttcatac ttacctcatt ctttaacagt
    27781 tttagaaaac gccgccatca tattgggtga gcatgaaatg agaaatcaga gagcaacggt
    27841 cattttgaaa gggttatctt acagttgtag ttattatcgc aagaagtatt ttagtcttac
    27901 aattgtagga atctgcttcc tgtcgcagag aactattctt a
//
LOCUS       DAZFWL020000053        27588 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFWL020000053 DAZFWL020000000
VERSION     DAZFWL020000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.053"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            40..1638
                     /gene="rtcR"
                     /locus_tag="ACHKNP_RS12070"
                     /old_locus_tag="pgaptmp_002414"
     CDS             40..1638
                     /gene="rtcR"
                     /locus_tag="ACHKNP_RS12070"
                     /old_locus_tag="pgaptmp_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HGU4704566.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETQVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYTRINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1620..2378)
                     /locus_tag="ACHKNP_RS12075"
                     /old_locus_tag="pgaptmp_002415"
     CDS             complement(1620..2378)
                     /locus_tag="ACHKNP_RS12075"
                     /old_locus_tag="pgaptmp_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HGU4704567.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2395..3225)
                     /gene="glpG"
                     /locus_tag="ACHKNP_RS12080"
                     /old_locus_tag="pgaptmp_002416"
     CDS             complement(2395..3225)
                     /gene="glpG"
                     /locus_tag="ACHKNP_RS12080"
                     /old_locus_tag="pgaptmp_002416"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HGU4704568.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3270..3596)
                     /gene="glpE"
                     /locus_tag="ACHKNP_RS12085"
                     /old_locus_tag="pgaptmp_002417"
     CDS             complement(3270..3596)
                     /gene="glpE"
                     /locus_tag="ACHKNP_RS12085"
                     /old_locus_tag="pgaptmp_002417"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HGU4704569.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3786..5291
                     /gene="glpD"
                     /locus_tag="ACHKNP_RS12090"
                     /old_locus_tag="pgaptmp_002418"
     CDS             3786..5291
                     /gene="glpD"
                     /locus_tag="ACHKNP_RS12090"
                     /old_locus_tag="pgaptmp_002418"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HGU4704570.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5343..5948)
                     /locus_tag="ACHKNP_RS12095"
                     /old_locus_tag="pgaptmp_002419"
     CDS             complement(5343..5948)
                     /locus_tag="ACHKNP_RS12095"
                     /old_locus_tag="pgaptmp_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704571.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     AWGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5951..6718)
                     /locus_tag="ACHKNP_RS12100"
                     /old_locus_tag="pgaptmp_002420"
     CDS             complement(5951..6718)
                     /locus_tag="ACHKNP_RS12100"
                     /old_locus_tag="pgaptmp_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704572.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6721..7431)
                     /locus_tag="ACHKNP_RS12105"
                     /old_locus_tag="pgaptmp_002421"
     CDS             complement(6721..7431)
                     /locus_tag="ACHKNP_RS12105"
                     /old_locus_tag="pgaptmp_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704573.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANYKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCTRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7447..8955)
                     /locus_tag="ACHKNP_RS12110"
                     /old_locus_tag="pgaptmp_002422"
     CDS             complement(7447..8955)
                     /locus_tag="ACHKNP_RS12110"
                     /old_locus_tag="pgaptmp_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU4704574.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVC
                     NILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLIL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLE
                     SSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMITRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(9081..11528)
                     /gene="glgP"
                     /locus_tag="ACHKNP_RS12115"
                     /old_locus_tag="pgaptmp_002423"
     CDS             complement(9081..11528)
                     /gene="glgP"
                     /locus_tag="ACHKNP_RS12115"
                     /old_locus_tag="pgaptmp_002423"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HGU4704575.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11547..12980)
                     /gene="glgA"
                     /locus_tag="ACHKNP_RS12120"
                     /old_locus_tag="pgaptmp_002424"
     CDS             complement(11547..12980)
                     /gene="glgA"
                     /locus_tag="ACHKNP_RS12120"
                     /old_locus_tag="pgaptmp_002424"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGU4704576.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(12980..14275)
                     /gene="glgC"
                     /locus_tag="ACHKNP_RS12125"
                     /old_locus_tag="pgaptmp_002425"
     CDS             complement(12980..14275)
                     /gene="glgC"
                     /locus_tag="ACHKNP_RS12125"
                     /old_locus_tag="pgaptmp_002425"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGU4704577.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14293..16266)
                     /gene="glgX"
                     /locus_tag="ACHKNP_RS12130"
                     /old_locus_tag="pgaptmp_002426"
     CDS             complement(14293..16266)
                     /gene="glgX"
                     /locus_tag="ACHKNP_RS12130"
                     /old_locus_tag="pgaptmp_002426"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HGU4704578.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16263..18449)
                     /gene="glgB"
                     /locus_tag="ACHKNP_RS12135"
                     /old_locus_tag="pgaptmp_002427"
     CDS             complement(16263..18449)
                     /gene="glgB"
                     /locus_tag="ACHKNP_RS12135"
                     /old_locus_tag="pgaptmp_002427"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HGU4704579.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18722..19825)
                     /gene="asd"
                     /locus_tag="ACHKNP_RS12140"
                     /old_locus_tag="pgaptmp_002428"
     CDS             complement(18722..19825)
                     /gene="asd"
                     /locus_tag="ACHKNP_RS12140"
                     /old_locus_tag="pgaptmp_002428"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU4704580.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            20017..20610
                     /gene="yhgN"
                     /locus_tag="ACHKNP_RS12145"
                     /old_locus_tag="pgaptmp_002429"
     CDS             20017..20610
                     /gene="yhgN"
                     /locus_tag="ACHKNP_RS12145"
                     /old_locus_tag="pgaptmp_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HGU4704581.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20667..22007)
                     /gene="gntU"
                     /locus_tag="ACHKNP_RS12150"
                     /old_locus_tag="pgaptmp_002430"
     CDS             complement(20667..22007)
                     /gene="gntU"
                     /locus_tag="ACHKNP_RS12150"
                     /old_locus_tag="pgaptmp_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU4704582.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(22011..22538)
                     /gene="gntK"
                     /locus_tag="ACHKNP_RS12155"
                     /old_locus_tag="pgaptmp_002431"
     CDS             complement(22011..22538)
                     /gene="gntK"
                     /locus_tag="ACHKNP_RS12155"
                     /old_locus_tag="pgaptmp_002431"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HGU4704583.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(22677..23672)
                     /gene="gntR"
                     /locus_tag="ACHKNP_RS12160"
                     /old_locus_tag="pgaptmp_002432"
     CDS             complement(22677..23672)
                     /gene="gntR"
                     /locus_tag="ACHKNP_RS12160"
                     /old_locus_tag="pgaptmp_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HGU4704584.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(23896..24591)
                     /gene="yhhW"
                     /locus_tag="ACHKNP_RS12165"
                     /old_locus_tag="pgaptmp_002433"
     CDS             complement(23896..24591)
                     /gene="yhhW"
                     /locus_tag="ACHKNP_RS12165"
                     /old_locus_tag="pgaptmp_002433"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HGU4704585.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(24714..25751)
                     /gene="yhhX"
                     /locus_tag="ACHKNP_RS12170"
                     /old_locus_tag="pgaptmp_002434"
     CDS             complement(24714..25751)
                     /gene="yhhX"
                     /locus_tag="ACHKNP_RS12170"
                     /old_locus_tag="pgaptmp_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU4704586.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            26084..26572
                     /gene="yhhY"
                     /locus_tag="ACHKNP_RS12175"
                     /old_locus_tag="pgaptmp_002435"
     CDS             26084..26572
                     /gene="yhhY"
                     /locus_tag="ACHKNP_RS12175"
                     /old_locus_tag="pgaptmp_002435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU4704587.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            26809..>27588
                     /gene="tssD"
                     /locus_tag="ACHKNP_RS12180"
                     /old_locus_tag="pgaptmp_002436"
     CDS             26809..>27588
                     /gene="tssD"
                     /locus_tag="ACHKNP_RS12180"
                     /old_locus_tag="pgaptmp_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HGU4704588.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGI"
BASE COUNT         6984 a         7289 c         6812 g         6503 t
ORIGIN      
        1 accgataatg aaatatcgtc ttttataagg atatctaaga tgcgtaaaac agtggctttt
       61 ggctttgtcg gtaccgtgct ggattatgcc ggacgcggca gtcagcgctg gtcgaaatgg
      121 cgtccgacgc tttgtttatg ccagcaagaa tcgttagtca tcgatcggct ggaattgttg
      181 cacgacaccc gctcgcgctc gctattcgaa acgcttaaac gcgatatcgc cagcgtttcg
      241 ccagaaacac aagtcgtggg cgttgagatt gaactgcata acccgtggga tttcgaagag
      301 gtttacgcct gcctgcatga tttcgcccgt ggttacgagt ttcagccaga aaaagaagac
      361 tatttaattc acatcaccac cggcacccac gtcgcgcaga tttgctggtt tctgctggcg
      421 gaagcgcgtt acctgcccgc ccggctgata caatcttcac ctccgcgcaa aaaagaacag
      481 ccgcgcggcg caggtgaagt gacgattatc gatctcgatt taagccgtta taacgccatc
      541 gccagccgct ttgccgagga acgccagcaa acgcttgatt ttcttaagtc cggcattgcc
      601 acgcgtaacc cccacttcaa ccgcatgatt gagcagatcg aaaaagtggc gatcaaatcc
      661 cgcgcgccga ttctgcttaa cggtccaacc ggcgcgggca agtcatttct ggcgcgacgc
      721 atcttcgagt taaaacaggc gcggcatcag tttagcggcg cgtttgtgga agtgaactgc
      781 gccaccctgc gcggcgatac cgccatgtcg acgctgtttg gtcatgtaaa aggcgcgttt
      841 accggggcgc gggaatctcg tgaaggttta ttacgcagcg ccaacggcgg aatgttgttt
      901 cttgatgaga ttggcgaact gggcgcagac gaacaggcaa tgctgctaaa agccattgaa
      961 gagaaaacct tttacccgtt tggcagcgat cgccaggtga gcagtgattt tcagcttatc
     1021 gccggaacgg tgcgcgattt gcgccagctg gttgccgaag gcaaatttcg cgaagatctg
     1081 tacacgcgga tcaatctctg gaccttcacc ctgccgggtc tacgccagcg ccaggaagat
     1141 attgaaccga acctggatta tgaagtggag cgccacgcct cactcactgg cgacagcgtg
     1201 cgttttaaca ccgaagcgcg gcgcgcctgg ttggcttttg cgacctctcc ccaggcaaca
     1261 tggcgcggta actttcgcga actttctgcc agcgtcacgc ggatggccac ctttgccact
     1321 agcggacgca tcactctgga cgtggttgaa gatgagataa accgtctgcg ctataactgg
     1381 caggagagcc gcccctccgc gcttacggcg ttgctgggcg cagaggcaga aaacatcgat
     1441 ctcttcgacc gtatgcaact ggaacacgtt atcgctatct gccgccaggc aaagtcgctt
     1501 tccgcagccg gacgccagct ttttgacgtt tcgcgccagg gcaaagccag cgtcaatgac
     1561 gcggatcggc tacgcaaata cctggcgcgt tttggtctga cgtgggaagc cgtgcaggat
     1621 cagcacagct ccagttgaat atggtggtcc gtcagcacct gcatcacgct tactggcggc
     1681 ggggcgtcgg tgtagacggc atctaccatg ctgatgctgc ccatattgac catcgcgtta
     1741 cggccaaatt tcgagtgatc gacaaccagc ataacgtggc gcgagttctc aataatggcg
     1801 cgtttggtgc gaacttcgtg gtaatcgaac tccagcagcg agccgtcgct atcgatgccg
     1861 cttatcccca gaatgccgaa atcaaggcgg aactgggaga taaaatcgag cgtcgcttcg
     1921 ccaatgatcc cgccatcgcg gctgcgtaat tcgccaccgg cgagaataat gcggaaatct
     1981 tcttttacca tcaacgtgtt agcaacattg agattgttgg tgacaatacg caaattgctg
     2041 tgattaagca gcgcgtgcgc caccgcttcc ggcgtggtgc cgatatcgat aaacagtgtc
     2101 gagccattgg ggatttgctc cgccactttg cgggcaatcc tctctttttc ttcggtctgg
     2161 gtggctttac gatcgtgcca cggcgtgtta accgaactgg aaggcagcgc cgcaccgcca
     2221 tggtgacgca ggatcaggtt ttgctccgcc agctcattga ggtcgcggcg aatagtctgc
     2281 gggctgacgg agaaatgctc taccagctct tcagtactga cataaccctg ctgtttaacc
     2341 agttcgataa taccgttgtg acgttgtgtt tgtttcattt ataaatccct ggaattattt
     2401 tcgttttcgc gcattgagcg aatcaacaaa agccatcgct aaacccacgg ctaacccggc
     2461 gatgtgtgct ccgttcgcca tcgacatccc aaacaaatca aaccatccgg cgacaatcca
     2521 gatcagcgca aagataatta acccacgttg caggtaaatg ccactttgcg gatcgcgttc
     2581 gccacgtagc cagacgtagc ccatcagcgc atacaccacg ccagaaagcc cgccaaacca
     2641 cggcccgctg aatttttgct gcacatagcc gcttaacagg gcgctaatga gagtaataac
     2701 aattagctta ccgctaccga ggcgtttttc caccgcaccg ccgagatacc accaccagag
     2761 caggttaaag aggatatgca tcagcgagaa gtgcattaac gcgtgggtga agtaacgcca
     2821 gaactcaaat ttcagtgttg gatcgaatgg ccaggccagc cataacatca cttcctgatc
     2881 gccgagaatt tgcatggcga taaacaccac cacgcaggcg atcatcacca cccaggttac
     2941 cggacctgcg cgttcacgca aggcagcaaa gaaaggataa cggcgataat gcagaccact
     3001 gccggtatgg cccgcctgcc agctggccgc cagataacgc ggatctgccg ggttttcgag
     3061 aaaacgcgcc agctccgccc gcacgcgctc ggcctgggac tcatccgcca gccagacatc
     3121 gctttggtta tgttgttgaa tcgtgaggat aacaccctgc gtcgccatat aatcaacaaa
     3181 cgcctgcgcc acgcgggggt tagcaaaaga ggtaatcatc aacatcgttg ctgtcgctta
     3241 ttccacacaa aaggggacag tataaagcgt tacgcgccgt acgccacctc tgcgggaaac
     3301 tgacgctgcc aggcttcaaa accgccgtca atgctataga ccacatcgta gccctgttgc
     3361 agcagatact gcgccgcgcc tttgctgcta ttgccgtgat aacacatcac catcaccgga
     3421 gtgtcaaagt cgttatcacg cataaacgcg cccagcgtgt cgttggttaa atggaaagcc
     3481 tgcaccgcat gccccatagc gaaactctgt ggatcgcgaa tatcgaccag caccgcctct
     3541 ttttcctgca acttctggtg cgcgtcggca acgttaatac attcgaacag atccatgcgt
     3601 ctctctttct ttacaaacaa gtgggcaaat ttaccgcaca gtttacgtcg aagcggcaga
     3661 taaacgccat aatgttatac atatcactct aaaatgtttt ttcaatgtta cctaaagcgc
     3721 gattctttgc taatatgttc gataacgaac atttataagc tttaacgaaa gtgaatgagg
     3781 gcagcatgga aaccaaagat ctgattgtga taggtggcgg catcaatggt gctggtatcg
     3841 cggcagacgc cgctggacgc ggtttatccg tgctgatgct ggaggcgcag gatctcgctt
     3901 gtgcgacctc ttccgccagt tcaaaactca ttcacggtgg cctgcgctac cttgagcact
     3961 atgaattccg cctggtcagc gaggcgctgg ctgaacgtga agtgctgctg aaaatggctc
     4021 cgcatatcgc cttcccgatg cgttttcgcc tgccacatcg tccgcatctg cgcccggcgt
     4081 ggatgattcg gattggtctg tttatgtacg atcatctggg taaacgcacc agcctgccgg
     4141 gatcaaccgg tttgcgtttt ggcgcaaact cagtgttaaa accggaaatt aagcgcggat
     4201 tcgaatattc tgactgttgg gtagacgacg cccgtctggt actcgccaac gcccagatgg
     4261 tggtgcgtaa aggcggcgaa gtgcttactc ggactcgcgc cacctctgct cgccgcgaaa
     4321 acggcctgtg gattgtggaa gcggaagata tcgataccgg caaaaaatat agctggcaag
     4381 cgcgcggcct ggttaacgcc accggcccgt gggtgaaaca gttcttcgac gaggggatgc
     4441 atctgccttc gccttatggc attcgcctga tcaaaggcag ccatattgtg gtgccgcgtg
     4501 tgcataccca gaagcaggcc tacattctgc aaaacgaaga taaacgtatt gtgttcgtga
     4561 tcccgtggat ggacgaattt tccatcatcg gcaccaccga cgtagagtat aaaggcgatc
     4621 cgaaagcggt gaagattgaa gagagtgaaa tcaactatct gctgaaagtg tataacgcgc
     4681 actttaaaaa acagcttagc cgtgacgata tcgtctggac ctactccggt gtgcgtccgc
     4741 tgtgtgatga tgagtccgac tcgccgcagg ctattacccg tgattacacc cttgatatcc
     4801 atgatgaaaa cggcaaagca ccgctgctgt cggtattcgg cggtaagctg accacctacc
     4861 gaaaactggc agaacatgcg ctggaaaaac tgacgccgta ttatcagggt attggcccgg
     4921 cgtggacgaa agagagcgtg ctgccgggtg gtgccattga aggcgaccgc gacgattacg
     4981 cagctcgtct gcgccgtcgc tatccattcc tgactgaatc gctggcgcgt cattacgcac
     5041 gcacttacgg cagcaacagc gagctgctgc tcggcaatgc gggggcgata agcgacctcg
     5101 gggaggattt cggtcatgag ttctacgaag cggaactgaa atacctggtg gatcacgaat
     5161 gggtccgccg caccgacgat gccctttggc gtcgcacaaa acaaggcatg tggctgaatg
     5221 cggatcaaca atctcgtgtg agtcagtggc tggtggaata tacgcagcag aagttgtcgc
     5281 tggcgtcgta aggatgatgc ccggttgaag tgcaaccggg ctatttggat tccttagact
     5341 attcattgta aataattgaa taacggcccc tgagctaaaa tcgccaaact taagcacgtt
     5401 acccaatcgc cccacttctt cctctggttt ctattttttc tgcaccaatg gcaataatat
     5461 ccccatgcaa aaccaagctg tgcactaatc gggccgacaa aatcagtcca aggctgcggc
     5521 caaaaagggg aaaaattcag gggaggccag acaaacgtca gaaagaaatt tcggatattt
     5581 tccagcaacg ctgatttctc tggagaaata taaagaatga aattcgagag aattaaccag
     5641 aaatagaccg gcgaaaagac cagccagcaa tgcagtctgt tttttaattc aggaggcgtt
     5701 ctgcgggaga attgccaacc gatgccagcc attacactga aaaaaagtgc atattcaacc
     5761 attaactcaa gttgtaattc ctgaagagaa atacgcacaa ccaggagcgg aaaggagaat
     5821 attatttctg ccagaaaagt taacgtgaat ataagtacgg ctaagacaat aaccgtcggc
     5881 cataccaata atggcacgat aacaaaaccg agaattacag ggggaatata aagccggaat
     5941 ttaatcattt ttattttcct ggtgattcgg atgatgcgtc atacatcgcg cataaacact
     6001 catcaattat ccagtcatct tcccagacct gatattctgt cattggttcg ggctgtttta
     6061 gcgggttctc aatcgcttgc cgcgctcttt ctttcagtaa aggatgatta tttctagccc
     6121 aggcattgat ttgggtggta atggcgttat cgatatcgtc ctggttatct ctgatataat
     6181 cctcacaata tctgttcaat aaacaggcaa gagaaacggt cgaccaaaag ccgggaactg
     6241 aattaatggc atccagcaat ggcgcaagag cgatatcggc acggaaaaac atttccagca
     6301 tatactgtaa ttcgacagct tcatcgccgg atacccaatc agtcatatac tggcaggcaa
     6361 atcgctgtaa aattgcccgt tcatccgcac gccagttttc tttgttggca aggttgatac
     6421 ggcgcagaga aaaaactacg tgaatattgt caaaatgccc ctgtgcaaca aattccagta
     6481 aacgcggcag aaaatagcgg acctcatcgc tattttgcgg atcaggccct gggctactat
     6541 tccaggcatt gattaaggaa tacgggatcg ctcgtaacga tgtactccgt aatgcctttt
     6601 gttcctgctg actcaggcaa tattcacaac aaaccacaaa tttctgcggc acccgataac
     6661 gacgaaaggt gtgataaatc tgctcaatta atgcctgcat ggcggggctg ggttgcatag
     6721 ttaccccctc gcccgctgat cgcgtgtaaa cggttgatag acaaaacgag cattcgccgc
     6781 gttcaggcga ataagggcgc tacgcgaaag ggaaaagctg gggcgagtgc aactattgca
     6841 gtctcgacag ctttcctctg cgcaattttc cactgagtaa tgacgcgtgt agagtttcag
     6901 ttcggcctgc ttatcgcgaa atccgcgtaa actgttattg ttatgaaatt tccaggcatc
     6961 tttaaagacc tgaaagatat agacatcctc aatccaggag tcacattttt taagcgggcg
     7021 ctttgctatt atcacccgct catccggatt tagcatcgcc gtaaattgta cggtgacgtc
     7081 atttgtcgtg ttcaggtcac attgaacatc tggtacatca ctgatatagg ggtcaaatcc
     7141 aaaatcagca ccacaggcac aaagacggct aatggtggcg gtatcgaggg aaaatcccgg
     7201 ccataccttg ctggaggtta agccaatatt taatgtgaca tcatatcgaa gcaatccctc
     7261 aagaccgtca gcaccgacga acttttcccc ttcatccagg gcctggttaa taagttgctg
     7321 aacaatgcct tcatcccagt atgccgattt gtagttggca atcgtaatat ccacgcctgt
     7381 gaagggatca gtaccggcaa ggcttgcaaa taatgtatta actaattcca tttttattat
     7441 ccagctttaa ggtagcgtct tatacatcgg gacataacct acatcttcca ccaaactctg
     7501 tccctgcggc gttaaaaacc actccaacaa tttttgtgtc tctgacgtcg tattttcccg
     7561 cgttatcata aaggcatcga cgatataggg atatttgccg ttgcgaatgt tttcggcagt
     7621 cggtgcaata ccgttaatcg ctagcagttt tatattttta tcggcgttca tttgtgttgc
     7681 gtaataacgg aaggtgtaac ctattgcgtt gtttgtatta cggtattcgg caacgacttt
     7741 aatcattccc tccatcatac ttgccacttc tgtttcctgc ggcgagatca tgcggacatt
     7801 tttcatgacc tgggattgca tcaccgtctg gctaccagag tcttccgggc gttgccaggt
     7861 ctggatctcc tgatcgttac cgccaacagt acgccagttg gtaattgcac cactgaagat
     7921 gtcacgcacc tgttgttcag tcagggaatt aaccggatta tccgcattga cgatgaaaac
     7981 aaacgcttca cgggcaaaag gcgtgtacag caaagtaacg cccgattcct cagcgcgttt
     8041 tttctgccca ccggaaggtt gcgccacaaa aataatatca gcatcacctt taacaatcct
     8101 gttatacgca tcgggggtac gagagctctc cagatattcc ctggtgtgaa aatcttccgg
     8161 tataacgctt aaagcataaa atgcagaagc ataaattggg tacgccgccg tagcaccatc
     8221 gagtcgcggc cagttttgcg taaactgaat ttgcggttta ccacgcagtg gtgtcagttg
     8281 attattgagt ttatcgggtc gccaggccca ggtatcaata ttttcgcgca caccgacgcc
     8341 gggattgagc tgtttatata aataaccctg ataaccagcg acaaaaccaa gcatgagaag
     8401 gataaacagg agggaattac ggcaacgtag cccggatgta ccggttaaag gatgtctggt
     8461 tggccggccg taccccagcg aaaaactgat atgcgcgcca acgggtataa tgactatcca
     8521 ccagtactca cctccgacta ctgagactaa caaaacgccc agccagggga gcataagatc
     8581 agccaaccaa cccgatccca tctggtgaaa ttgataatct gccagatgca gcgcgattat
     8641 ccagccaacc aggattaata aaccgggtac taaaaaaaga cattgtcgcc agacggctat
     8701 ttctcttttt tctggtgcac tatcgcctgc ccatactcgc cccgagaaaa atagcattaa
     8761 tgcgcccagc gccagcataa aaatgttgag cagaaggtaa cttgttggcg tgataaggat
     8821 attacaaact tcaataatac cggcaagtcc gacacccagc atagcaacaa ccgccgccaa
     8881 aaattgcgtc agtatgggga tgccgaaaaa agtcattacc agcgaggaca aaatccattt
     8941 tctgttttgc attattcttt ccattctttt tgaatggtga aattatactc cccgagtccc
     9001 cttgcccctt ctggacactt ttccgaaatg atggcggaaa aaaacgggag cctttggccc
     9061 cgttctattt attggtgaac ttacaatctc accggatcga tatgccagat atgatcggcg
     9121 tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt cagcatcgct
     9181 tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg acaatcgaca
     9241 tagctgcgat aatccgccag aacctgatag tgatcgccga agttgatcag cgaatcaacc
     9301 agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat ttgcgtcagc
     9361 acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc ctgacgacgc
     9421 agttcttcca cttcttccgc agtgttacca aagataaaga tattgtcagc accgacatga
     9481 tccagcatct cgacgttcgc accgtccagc gtaccgatag tcagcgcgcc gttaagcgca
     9541 aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg ttcagacaga
     9601 tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat gaacacgact
     9661 ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc attgatcaaa
     9721 tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa aatattcacg
     9781 cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt aatcacatgc
     9841 aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac atcgaacaac
     9901 gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag acgctttttg
     9961 ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca gtgttgttgc
    10021 agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc gtccagcacg
    10081 gctgaaagcg atgggttcgc taccgctagc cagcgacgcg gcgtcacacc gttggtgacg
    10141 ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg caccatcaga
    10201 ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc cagccaggcc
    10261 atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag cagatcggta
    10321 tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc aaagatgatc
    10381 tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc cagcgcctcg
    10441 ctcatcagcg tatggttagt gtaggagaag acctgacaac acacctcaaa cgcgtcgtcc
    10501 cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga cagtaccgga
    10561 tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt tttatgcaac
    10621 tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccaggaagta ttcctgacgc
    10681 aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg agatacgttc
    10741 tcggagtggt ttttatcttc cactgccgcg aagtagtcac cctggttgaa tttaccgagg
    10801 ttaatttcgc tactggcttg cgcactccac aaacgcaacg tgttggtcgc gtcggtgtcg
    10861 taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc aatccagcgc
    10921 gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg cgtgttgtgg
    10981 cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc tttctggcta
    11041 ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta accgcgcccc
    11101 ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc caggccaccg
    11161 ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa ccccatcgct
    11221 tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt ggagagcgta
    11281 cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg cgacaactgg
    11341 gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa taacgttgcg
    11401 ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa catcagcttg
    11461 taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata tgtaaacgga
    11521 gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc acggtacgac
    11581 ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac aaaccgccac
    11641 agtgaaggac gggaccacag tacaaaagca cgtcgaatag cccgtaacag cgaccaggca
    11701 ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc aagagaacag
    11761 tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt accgtacttc
    11821 aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag aatgacgtcc
    11881 gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac gcccacctgg
    11941 ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc gcccgcgccg
    12001 agtagcgcca gctgcccgcc ctgctccaga aggcccggta aggcttccag caccaggtcg
    12061 agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt atcatcaacc
    12121 ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc caacgtatcg
    12181 cgggtgtagc gcgaggccaa cagtaagtcc gtctctggac tccagatttt ctcgtccacg
    12241 ccgttcagta cgccggaaag acgcccttca cggtgacgct gttgcaacag accttccata
    12301 ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactaac cgccgtaatg
    12361 tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa ttccagccca
    12421 tgaatattaa agaatgacca tggcaattgg atgtcattca tatgatgtgc ataaaacatg
    12481 ccttgatagg ccaggttgtg cacagtaaac accgacttcg ccggacgccc gcgcgccgcc
    12541 agatacgcag gcgcaaggcc tgcatgccag tcgtgcgcat gcaccacatc aggacgccag
    12601 aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc aaaacgcaat
    12661 acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg acgatcatag
    12721 agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa caacagcgtg
    12781 atatgtccgg cgaaggtatc acgacgggat actacctgcg catcggtaac gccacggcga
    12841 atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat ttgtgctgcg
    12901 ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa catctctgaa
    12961 catacatgta aaacctgcat tatcgctcct gtttatgccc taacttccgt agcatttcgc
    13021 gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct tcctctgcgt
    13081 tttcaccaat caccatgcct tccggaataa cacaagcacg atcgatgacg cagcggcgca
    13141 gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca atgttgcaga
    13201 atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa ccggagatca
    13261 cacagccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag cgatcctgca
    13321 cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg cgatcgtaca
    13381 tatccagttc cggcaccaca gaggccagat cgaggttcgc tttccagtaa gcttccagcg
    13441 tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa gagagcggga
    13501 acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct ttgccaaagt
    13561 cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga tagtcggcgt
    13621 caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc attgacggtg
    13681 ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc gccataacgc
    13741 caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa cgtgcgcctt
    13801 tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg tcgcccgcca
    13861 ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg gtgaccgcat
    13921 ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc agcagatcga
    13981 caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc tgcaccagag
    14041 tgtgggactg gtactgggtg atcacgccca tacgacggat cccggagttg atgcagttag
    14101 acagcgcaaa gtcgataatg cggaacttac cgccgaagtg tacggccggt tttgctcgct
    14161 tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg gcaacagatt
    14221 tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta accatgacta
    14281 actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct gccagacagc
    14341 cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact ccccagcagg
    14401 taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc gatccgagag
    14461 cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag gttgagcata
    14521 tcgatttagc caacggacat tgccgtcgcc ttcttcccac cagcgattct ccaccaaagc
    14581 gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac cactgcttgc
    14641 ctgcgaccag tccaaccagg ttaattggtt atcctgacag taggcattgt tattgccatg
    14701 ctggctgtga ccatgttcgt caccggccag taacatcggc gtaccctggg agagcaacaa
    14761 cgttgttaac agggcgtgaa tgctgtcgcg ccgccgttca accaggtcaa gagaaccgcc
    14821 taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc gattttcttc
    14881 tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg taaaaccgtc
    14941 atgcgcggtg acgagattaa tcgcggcact cggcagacga ccattacgtt taaaaacatc
    15001 gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat gtagccagaa
    15061 acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg ggaaatttcc
    15121 cacctgataa ccaccaggag cgatatccca cggttcagca attaacttca cctgcgagag
    15181 caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact ctggcgtacg
    15241 gcccatgact gccgccagat caaagcggaa accatcgacg tggcaggttt ctacccaata
    15301 acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga gcgtgttgcc
    15361 gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc tacggttatc
    15421 gatcccacgc agcgagaata acgggccgtc gaggtccagt tccgcactat ggttgagcac
    15481 gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat cgcgaaactc
    15541 atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca tcgccaccgg
    15601 gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac tggcaaactg
    15661 cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt tgatcatcac
    15721 cggatgcccg agggctttat aagtgccacg gatctcgacc gggatctccg ggtgcaagta
    15781 cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg tgcgcggcgg
    15841 ggcatcatct tcccagtcat agtgatcaac caccactacg catttcggcg caatggcggc
    15901 gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat ctttaaactc
    15961 cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa agcgatgccc
    16021 ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg ggcgcgcatc
    16081 cggcagataa ccgtgccaaa tgtcgccact gtgccctggc aagtcatagc gatgttcctg
    16141 gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga aaagtgtgaa
    16201 gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa tggcgagttg
    16261 tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagcgt caggcttagt
    16321 gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc attgcctgca
    16381 ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt gcccggctgg
    16441 tttatgccga agcgataatc atgacgcggt accggcgtaa agttactggc aacgatgatt
    16501 tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt gtcatccacc
    16561 accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt atggtggcgg
    16621 taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt atcgccgcct
    16681 tccaacagat gccagtcgag gctggcgtca tggttccact cgcggccctg ggcaaattcg
    16741 ttacccatga acagtagttt cttgcccggg aatgcccaca tccagccata gtaggcgcgc
    16801 aggttcgcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga ttttttaccg
    16861 tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt gtagagaatc
    16921 ccgaaggtca gtttatcgtg atgatactga cgataaaccg ggtcgagctt catgtagtcc
    16981 agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc gcccatatcc
    17041 tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac cgcaccggaa
    17101 acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc aagattctcg
    17161 cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc gcgataaatc
    17221 attgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat ccagtaaagc
    17281 gcgttaccga cgaggaagtt actgacttca cggcgaccat agttgtagat cagcgtgttc
    17341 cagtcctgat gatagccttc acgcggatcg ctgtgttcat acaagttcgt gccatcaaat
    17401 tcggcaagcg caaagtcatc agtcgggaag tggcctggca cccagtcgag aatcacgttc
    17461 agaccagctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt accaaaacgg
    17521 cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa gggatgctcg
    17581 ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg caccagttga
    17641 tcggccagct cgcggtagct caaccagaaa ttgttgtcgg tgtggcgacg ccaggaaccc
    17701 aggtgaactt cataaataga gattggcgca tcaaactgat tcgctttttt gcgctcttca
    17761 gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg gcgcatttgc
    17821 gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc aatcatctcg
    17881 tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccagat gccgctctct
    17941 ttacgcaggc gcatcgggtg acggcgaccg tcccagtagt tgaattgccc aaccaccgag
    18001 acccgacggg cgtttggagc ccagacagag aaacgcgtac ctgtgacgcc atccatagta
    18061 tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga taatagccag
    18121 gcatccattt cctggattag cggaccaaaa cggtaaggat catcaatcag gttttgctga
    18181 ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg aatgacgccg
    18241 ctaaagaatc cccgtgagtc gagacactcc agttttgcga gtttgcgccc ggttttcggt
    18301 tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag tcccgcggtg
    18361 gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat tagcgcgtta
    18421 atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg tgtcacccca
    18481 tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg cgcagcactc
    18541 tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc atgtattcag
    18601 ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga aacatctgcg
    18661 cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta atgaataaag
    18721 attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac agcagctggt
    18781 cgcccacggt aaaggctgac aggaactctg gtcccatatt cagcttacgc aggcggccta
    18841 ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata gtgatttccc
    18901 gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct tccacggtcg
    18961 gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag cgcaatgccc
    19021 cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg atcttgttgg
    19081 tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt ttgtcgatcc
    19141 acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc tcaccgctac
    19201 gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg gtcgcgagtt
    19261 catctgccac atggccatac agatggccca tctgggttaa taactcacgc atatgtcgcg
    19321 caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca agatcattgg
    19381 cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg ccaacaaaag
    19441 tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg tcaagaatga
    19501 tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa ccttgccatc
    19561 cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga caggtcacaa
    19621 tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg ccagtggttc
    19681 cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca gggcgaatgg
    19741 cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc ataccgcgcc
    19801 agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg tgctgtataa
    19861 atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa agtcgcaagt
    19921 gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt tgttagcacg
    19981 cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat ttctgcagca
    20041 gttttattga tcctgattat ggatccgctc ggaaacctac ctattttcat gtccgtactg
    20101 aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct tattgctctc
    20161 ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct cagcctgcga
    20221 gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat taagatgatt
    20281 ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc atttatcgtg
    20341 ccgttggcta ttccgttagt cgccgggccg actattctcg ccacgctgat gttgctgtct
    20401 caccagtacc caaatcagat gggacatctg gtgattgctc tgctgctggc ctggggcggc
    20461 acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga gaaaggggtg
    20521 aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaaccca gatgttcctc
    20581 gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa gaacccggta
    20641 gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat catcccaacg
    20701 atggcaccga cagtgccgag gatggtttcc atcatggtcc aggttttcag cgtttcggct
    20761 tcggtcgcgc cggtaaattt accgaacaac cagaaaccgg cgtcgttaac gtggctgaca
    20821 acaatcgaac caccagcgat acaaatcgac agcgccgcca tttgcgcacc ggagtagttc
    20881 agttgttcaa taaccggcat caccagtcct accgccgtta aacaggctac ggtggcagaa
    20941 ccctgaatga tgcgcactgc agcggccagc acgaagcagg tgatggctat cggcaggccc
    21001 atgccagtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag cacctgtttg
    21061 aacacgccac ctgcaccaat caccagcaga atgatccccg ccggttgcag cgcgtgaccg
    21121 caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc ataaatcgct
    21181 accagacaag caaccagaat cgcggtaaac ggatgaccaa taaactcgaa ccattcgtaa
    21241 gcggtagagc cttctggcac aaaacgcgcg gcaatggttt tcagccccac cagcaccagc
    21301 ggcaacagga tcagcgacag gctgaatccg aaagatggca ttttgccttc gccgagatgc
    21361 ggttcgctga tgtcgtcagg aatatgcaac tccacgtagc ggctgatgaa attaccccac
    21421 agcggcccgg caataatcat tcccggaatt gccgcacaca ggccaatcag gatcatccag
    21481 ccaaaatcgg cgttcatctg cgatgccagc agcattggcg ctggccctgg caccaggaac
    21541 gccgccgctg ccgccacgcc tgcgaataat gggattacca gcttcaccag gttcgtaccg
    21601 gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa caatcgccac ttcaaagaac
    21661 agcggtagcg cacagaccag ccccgcaagg ccgatggcat aatgcgcgcg gctgtgaccg
    21721 aaggatttaa gcattttgac ggcaatctga tcgactgcgc cggtttcatg taagatcttg
    21781 ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggtgcc tcccatccct
    21841 ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc agcccccatg
    21901 gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa aaacagcagc
    21961 agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac ttatttgcct
    22021 tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc gatatccacc
    22081 accagtacat cagtttcgtc cgcacccggc tcctgcagcg tttcaaactg cgtcaccaac
    22141 atttgggttt taaagaaatg gcctttgcgc gctttcaggc ggctttcaat cacgtcaaaa
    22201 tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa gtcgcgatag
    22261 tgttttttca atgcagaaca gacgatcagt gacactttat tagtgcgctg catagcaaac
    22321 gcggcgtcgt tcagcgcctg caaccacggt ttgcgatcgt cgtcattcag cggttcgcca
    22381 gacgccattt tttcgatatt gcgccgtgga tggaggaaat cgccatcaag aaacgcggca
    22441 tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga tacgcccatc
    22501 aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc acaatacaag
    22561 aattaaaaat gttacgggta actgttatcg gtaacattgt ccagccggac aatatcagaa
    22621 gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt gtaggcttaa
    22681 atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt cacagattcg
    22741 ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc acgcggcgtc
    22801 agcacgctcg ctaaccgcgg ctccatcacc tggccaatgt catgaccgtg gaaaccggca
    22861 atcgccatat cgtcaggaac cttcaatccc agacgctgac attcaaacgc cgcgccgacc
    22921 gccaggtcat cgttggtaca gaacacgcca tccagctgcg gatattcccg ccgcgcctgg
    22981 cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac gctatacggc
    23041 accagacctg catccagcat cgcctgttcg tatcccttct gtttgatgat agtacgttcg
    23101 tcgagacgcg cgccgagata ggcaatgtgg caatgcccgc gagcaataat ggcggtggtc
    23161 atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca cggcgactgg
    23221 ctgtccatga gttccaccac cgggatcccc gccacttcaa tcatctttaa ggtgcgcggc
    23281 gtgtgggtac gttcggtgag gatcagtccg tcgatattcc atgagagcat ggattcgaga
    23341 cgttcttgct ccatttccgg tttataaccg tagtgcgcca gcatggtctg ataaccgtgc
    23401 gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt ggtgagagaa
    23461 ggtaacagga cgccaatcgc ccggctggtg gcgttagaga ggatatcggg cgcacgattg
    23521 ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc gacggaaacc
    23581 tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac acggtcagcc
    23641 acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa gagatagatt
    23701 ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct tgtttacaat
    23761 tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc aaaaccccgg
    23821 aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg caggcttcaa
    23881 aaatgccgtg gagctttaaa ccggcggcag atcgaacagt aacacttcgc tgtcgctatc
    23941 cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg tcgaggcttt
    24001 cacgccgtta atggtgacat tgcctttcac cacctggatc cagacgcggc gttcagcggc
    24061 aatctgatgc accgactgct catctttcag caacgcccag cggtacagtt ccatatcctg
    24121 atgcactttc aatgaaccat ctcgcgcatc cggcgagagc accagctgtt tgccctgtac
    24181 ggcatcgaag cgacgctgtt cataacgcgg cgtaataccg ttttcttcag gcatgatcca
    24241 gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt gacgaatacc
    24301 cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat tgcccatgct
    24361 gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat ctttatgcgg
    24421 gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc gcagcgcgga
    24481 gaagcccata aagttcggat cgtaatagtt ggcaaaagag aaagtatgcc aggagtccag
    24541 ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca tttgtattcg
    24601 ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat gatagagggt
    24661 gaaaattgac ccctctgttc aaaaaaattg aactattcga ggggccatca aacttaattc
    24721 gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat ttccagattg
    24781 gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt ttgatacaac
    24841 gcatcataaa cgcgcccgta atcgcccatc tccggcttca tctcttctct gaccgtcacg
    24901 ccctcgtcat tgacatactc cagcacaccg accgaattat ccgctgcgaa tcccggttcg
    24961 cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta tttaataaac
    25021 gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag atggctggtt
    25081 ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc gtcaggattg
    25141 gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc gaacagagaa
    25201 ataatctggt ccatcgtatg cacaccaagg ccataaaacg cgccatcctg cggcagccca
    25261 ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc aacaatctct
    25321 cccaacttgc cactttcaat cgcttttttc gctgtcagga agcaggagtc aaagcgacga
    25381 ttctgatacg gcgtgacggt cagccctttg cttttcgcca acgcaaacag ctctttcgcc
    25441 tgcgcaagtg tcggagtgaa cggtttttcg accagcacat ttttcccggc ttccagcgcg
    25501 cgcttcgcgt actcgaagtg gctgtccgcg tgggtgcaga caacaaccag cttaacatcg
    25561 ggatcgttta gtacttcgtc gagatcgctg gtgaaatgga tatgggaata aatgggagcc
    25621 tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct atccttgcgg
    25681 ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat aaaggcgcag
    25741 ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg caaagcggac
    25801 gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa attgagaacg
    25861 aaagatcaaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc aatgtgagca
    25921 atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat aataatcatt
    25981 ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc atttgaaagt
    26041 caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg agatagtaat
    26101 acgccacgca gaaacacggg attacgaggc catcaggcag attcacgccc agccggaggt
    26161 gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg agcgactcgc
    26221 cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg tgggccatct
    26281 caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg gtatctgtgt
    26341 cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga tgattgaaat
    26401 gtgcgacaac tggttgcggg tagatcgcat tgaactaacc gtgtttgtcg ataacgcccc
    26461 ggcaattaag gtctataaaa aatacggctt tgaaattgaa gggactggta agaagtacgc
    26521 attgcgtaat ggtgaatatg tcgatgcata ttatatggcg cgggtgaagt aagatagtgc
    26581 cctttttctg agatggaaaa agggtgtcat tcaaaatcga cataccttcc tttaaggtat
    26641 ttatttgccc aatacatata ttgagattta actcataatt acttcctaaa gtgtaatatt
    26701 ttatttttta atatatacgc ctacaatttc ctggagtaaa taaataacaa ttaacaagca
    26761 taatattgcc attgataaaa tagcatgcca taaaaggact tttcagggat gagtaatatt
    26821 gtttacctga cagtaacggg agaacaacaa ggaagcatct ccgcaggttg tgggacttct
    26881 gagtctacag gtaatcgttg gcagagcggg catgaggatg aaatatttac attctcactc
    26941 ttaaataata ttaataatac ggggcttggt tcacagttcc atggtataac attttgtaaa
    27001 ttaattgata aaagcactcc attatttatt aattccatta acaataatga acaattattt
    27061 atgggatttg acttctatcg aataaataga tttggtagat tggaaaagta ttattatata
    27121 caactaagag gcgctttttt atcggctatt catcaccaga tcattgaaaa ccaactggat
    27181 acagaaacaa taactattag ttatgaattt atcctctgtc aacatcttat cgcaaatacc
    27241 gagttcagct atttggcact ccctgaaaat tataaccgtt tgtttttacc aaattcaaaa
    27301 aaccaaacaa ataatcgttt caaaacgtta aacagcaaag ctattggcag gctacttgct
    27361 gctggtggcg tatacaatgg gaacattgaa ggattcagag atactgcgga aaaactgggt
    27421 ggagatgcaa taaaaggcta tgatcaaata ctaaatgaaa aaacagcggg catagcgata
    27481 gcaacagcat ctattctttt aacaaagcgt tctaatgttg atacatatac agaaataaat
    27541 agttacttag gcaaacttag aggtcaacaa aaacttcttg atggtata
//
LOCUS       DAZFWL020000054        27206 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFWL020000054 DAZFWL020000000
VERSION     DAZFWL020000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.054"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..403
                     /locus_tag="ACHKNP_RS12185"
                     /old_locus_tag="pgaptmp_002437"
     CDS             <1..403
                     /locus_tag="ACHKNP_RS12185"
                     /old_locus_tag="pgaptmp_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGU4704589.1"
                     /translation="SIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFEPSDVEYY
                     FEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSN
                     QIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(442..1341)
                     /gene="nhaR"
                     /locus_tag="ACHKNP_RS12190"
                     /old_locus_tag="pgaptmp_002438"
     CDS             complement(442..1341)
                     /gene="nhaR"
                     /locus_tag="ACHKNP_RS12190"
                     /old_locus_tag="pgaptmp_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HGU4704590.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(1407..2573)
                     /gene="nhaA"
                     /locus_tag="ACHKNP_RS12195"
                     /old_locus_tag="pgaptmp_002439"
     CDS             complement(1407..2573)
                     /gene="nhaA"
                     /locus_tag="ACHKNP_RS12195"
                     /old_locus_tag="pgaptmp_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HGU4704591.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3159..3311
                     /gene="mokC"
                     /locus_tag="ACHKNP_RS12200"
                     /old_locus_tag="pgaptmp_002440"
     CDS             3159..3311
                     /gene="mokC"
                     /locus_tag="ACHKNP_RS12200"
                     /old_locus_tag="pgaptmp_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HGU4704592.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3415..4545)
                     /gene="dnaJ"
                     /locus_tag="ACHKNP_RS12205"
                     /old_locus_tag="pgaptmp_002441"
     CDS             complement(3415..4545)
                     /gene="dnaJ"
                     /locus_tag="ACHKNP_RS12205"
                     /old_locus_tag="pgaptmp_002441"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGU4704593.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4634..6550)
                     /gene="dnaK"
                     /locus_tag="ACHKNP_RS12210"
                     /old_locus_tag="pgaptmp_002442"
     CDS             complement(4634..6550)
                     /gene="dnaK"
                     /locus_tag="ACHKNP_RS12210"
                     /old_locus_tag="pgaptmp_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGU4704594.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6927..7331
                     /gene="yaaI"
                     /locus_tag="ACHKNP_RS12215"
                     /old_locus_tag="pgaptmp_002443"
     CDS             6927..7331
                     /gene="yaaI"
                     /locus_tag="ACHKNP_RS12215"
                     /old_locus_tag="pgaptmp_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HGU4704595.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7357..8070
                     /gene="msyB"
                     /locus_tag="ACHKNP_RS12220"
                     /old_locus_tag="pgaptmp_002444"
     CDS             7357..8070
                     /gene="msyB"
                     /locus_tag="ACHKNP_RS12220"
                     /old_locus_tag="pgaptmp_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU4704596.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELAELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8219..8785
                     /gene="satP"
                     /locus_tag="ACHKNP_RS12225"
                     /old_locus_tag="pgaptmp_002445"
     CDS             8219..8785
                     /gene="satP"
                     /locus_tag="ACHKNP_RS12225"
                     /old_locus_tag="pgaptmp_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HGU4704597.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8820..9407)
                     /gene="mog"
                     /locus_tag="ACHKNP_RS12230"
                     /old_locus_tag="pgaptmp_002446"
     CDS             complement(8820..9407)
                     /gene="mog"
                     /locus_tag="ACHKNP_RS12230"
                     /old_locus_tag="pgaptmp_002446"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HGU4704598.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9522..10475)
                     /gene="tal"
                     /locus_tag="ACHKNP_RS12235"
                     /old_locus_tag="pgaptmp_002447"
     CDS             complement(9522..10475)
                     /gene="tal"
                     /locus_tag="ACHKNP_RS12235"
                     /old_locus_tag="pgaptmp_002447"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU4704599.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10754..12184
                     /gene="yaaJ"
                     /locus_tag="ACHKNP_RS12240"
                     /old_locus_tag="pgaptmp_002448"
     CDS             10754..12184
                     /gene="yaaJ"
                     /locus_tag="ACHKNP_RS12240"
                     /old_locus_tag="pgaptmp_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGU4704600.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     IQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            12254..13030
                     /gene="yaaA"
                     /locus_tag="ACHKNP_RS12245"
                     /old_locus_tag="pgaptmp_002449"
     CDS             12254..13030
                     /gene="yaaA"
                     /locus_tag="ACHKNP_RS12245"
                     /old_locus_tag="pgaptmp_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGU4704601.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(13183..13479)
                     /gene="yaaX"
                     /locus_tag="ACHKNP_RS12250"
                     /old_locus_tag="pgaptmp_002450"
     CDS             complement(13183..13479)
                     /gene="yaaX"
                     /locus_tag="ACHKNP_RS12250"
                     /old_locus_tag="pgaptmp_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HGU4704602.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(13693..14979)
                     /gene="thrC"
                     /locus_tag="ACHKNP_RS12255"
                     /old_locus_tag="pgaptmp_002451"
     CDS             complement(13693..14979)
                     /gene="thrC"
                     /locus_tag="ACHKNP_RS12255"
                     /old_locus_tag="pgaptmp_002451"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGU4704603.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(14980..15912)
                     /gene="thrB"
                     /locus_tag="ACHKNP_RS12260"
                     /old_locus_tag="pgaptmp_002452"
     CDS             complement(14980..15912)
                     /gene="thrB"
                     /locus_tag="ACHKNP_RS12260"
                     /old_locus_tag="pgaptmp_002452"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGU4704604.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAEIF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15914..18376)
                     /gene="thrA"
                     /locus_tag="ACHKNP_RS12265"
                     /old_locus_tag="pgaptmp_002453"
     CDS             complement(15914..18376)
                     /gene="thrA"
                     /locus_tag="ACHKNP_RS12265"
                     /old_locus_tag="pgaptmp_002453"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HGU4704605.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDRFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18403..18522)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18458..18523)
                     /gene="thrL"
                     /locus_tag="ACHKNP_RS12270"
                     /old_locus_tag="pgaptmp_002454"
     CDS             complement(18458..18523)
                     /gene="thrL"
                     /locus_tag="ACHKNP_RS12270"
                     /old_locus_tag="pgaptmp_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HGU4704606.1"
                     /translation="MKRISTTIITTITITTGNGAG"
     gene            complement(18737..19423)
                     /gene="yjtD"
                     /locus_tag="ACHKNP_RS12275"
                     /old_locus_tag="pgaptmp_002455"
     CDS             complement(18737..19423)
                     /gene="yjtD"
                     /locus_tag="ACHKNP_RS12275"
                     /old_locus_tag="pgaptmp_002455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU4704607.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19823..19963)
                     /gene="yjjY"
                     /locus_tag="ACHKNP_RS12280"
                     /old_locus_tag="pgaptmp_002456"
     CDS             complement(19823..19963)
                     /gene="yjjY"
                     /locus_tag="ACHKNP_RS12280"
                     /old_locus_tag="pgaptmp_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HGU4704608.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20059..20775
                     /gene="arcA"
                     /locus_tag="ACHKNP_RS12285"
                     /old_locus_tag="pgaptmp_002457"
     CDS             20059..20775
                     /gene="arcA"
                     /locus_tag="ACHKNP_RS12285"
                     /old_locus_tag="pgaptmp_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HGU4704609.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20835..22187)
                     /gene="creD"
                     /locus_tag="ACHKNP_RS12290"
                     /old_locus_tag="pgaptmp_002458"
     CDS             complement(20835..22187)
                     /gene="creD"
                     /locus_tag="ACHKNP_RS12290"
                     /old_locus_tag="pgaptmp_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HGU4704610.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(22245..23669)
                     /gene="creC"
                     /locus_tag="ACHKNP_RS12295"
                     /old_locus_tag="pgaptmp_002459"
     CDS             complement(22245..23669)
                     /gene="creC"
                     /locus_tag="ACHKNP_RS12295"
                     /old_locus_tag="pgaptmp_002459"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HGU4704611.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23669..24358)
                     /gene="creB"
                     /locus_tag="ACHKNP_RS12300"
                     /old_locus_tag="pgaptmp_002460"
     CDS             complement(23669..24358)
                     /gene="creB"
                     /locus_tag="ACHKNP_RS12300"
                     /old_locus_tag="pgaptmp_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HGU4704612.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQAPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTIDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24371..24844)
                     /gene="creA"
                     /locus_tag="ACHKNP_RS12305"
                     /old_locus_tag="pgaptmp_002461"
     CDS             complement(24371..24844)
                     /gene="creA"
                     /locus_tag="ACHKNP_RS12305"
                     /old_locus_tag="pgaptmp_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HGU4704613.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25055..25924
                     /gene="robA"
                     /locus_tag="ACHKNP_RS12310"
                     /old_locus_tag="pgaptmp_002462"
     CDS             25055..25924
                     /gene="robA"
                     /locus_tag="ACHKNP_RS12310"
                     /old_locus_tag="pgaptmp_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HGU4704614.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25921..26568)
                     /gene="gpmB"
                     /locus_tag="ACHKNP_RS12315"
                     /old_locus_tag="pgaptmp_002463"
     CDS             complement(25921..26568)
                     /gene="gpmB"
                     /locus_tag="ACHKNP_RS12315"
                     /old_locus_tag="pgaptmp_002463"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HGU4704615.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26620..27141
                     /gene="yjjX"
                     /locus_tag="ACHKNP_RS12320"
                     /old_locus_tag="pgaptmp_002464"
     CDS             26620..27141
                     /gene="yjjX"
                     /locus_tag="ACHKNP_RS12320"
                     /old_locus_tag="pgaptmp_002464"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HGU4704616.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
BASE COUNT         6635 a         7215 c         6614 g         6742 t
ORIGIN      
        1 atccatcgac actggacgat ttgtactgtc tcgtatcaat aatgaaaatt atcagtttca
       61 tcatataaga tttgactgca tccagggcga gcaaggtgaa ttagtttttg aacccagtga
      121 tgttgaatat tactttgaac cagtgacgat acaggagtca ggaaccagca tattaaaaaa
      181 tgagttagaa aacagtgagg acggtgcggg tcaaataggc tttcaactct ccaatgatgg
      241 tacgaatgaa atccagtatg gtaaaagcaa ttactactct tttcagcatc cccatgaagg
      301 aagtaatcag attcctctat ttattcgccc gcgtacatat ggaaataatg tctcaagcgg
      361 gcaaataatg agccgagtca aaatcgtggt tatgtacaac taatctatct cccccacctt
      421 aactttgggg ggaggtgccg attaacgcgc cgctggacta aaaagcgcag aataatccgt
      481 attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa tagcatggta
      541 ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg cataaaagtc
      601 atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa aagctttcat
      661 caacgcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg agttaaacca
      721 gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc gccgttcttc
      781 cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac tcacgccaca
      841 ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg gacagtcaga
      901 aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt gggtggattc
      961 gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta cgctactgac
     1021 caggcgtttg gaaagtgcat cagccacacc aacgtcaaac aataaattgg attctttgcg
     1081 atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg catagcgata
     1141 gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa ataatttgcc
     1201 ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag ttaaataaag
     1261 cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt aatacaagtg
     1321 gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa agggagccgt
     1381 ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac gcgtaaccag
     1441 ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt cgcccagtta
     1501 atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga catagtaaaa
     1561 ccgataccgc acaggatccc caccgccata atttgctgat aagtcgttcc ctcaggcaga
     1621 tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag cggtttgcca
     1681 atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc cagcgtgaca
     1741 ccttgcagtg aaacgccagc attagcaaat gcaaacagcg gcaaaatcag atacgccacc
     1801 cacgggtgta acacatgctc cagtcgcttc gctggagaac gcccatgctt ctctttcaaa
     1861 ggaataaaga agccgacaat tacccccgcc agggttgcgt gaacccccga tttcaacacc
     1921 gcagtccaca acactacgcc aaccagaata tagacgcccg tgcggcgtac accacacaga
     1981 ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagagaggc catcgataag
     2041 tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat aatagccaga
     2101 gccatcaaaa agatcttcag cgctaacgga acacgacttc ccaacagcgc cagcacacca
     2161 agtgcaaagg caatgtcagt agccgccggg atcgcccacc cttcgcgggt aatcggatcg
     2221 gcatagttaa aggccagata gagtaatgcc ggaacaatca tcccaccaat agcggcgata
     2281 actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc acgtttaact
     2341 tccagaccga ctaacaggaa aaataccgcc atcagcgcgt catttatcca taacagcatg
     2401 tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag aaagtcgtga
     2461 taccatccac tggttgcgcc gctattggcc ataatcatcg ccaggatagc ggcaatgata
     2521 agaataatgc ctcccgaggc atcactgcta aagaatcgat gcagatgttt cactttttat
     2581 ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacaaatc atcgttaaaa
     2641 tcagattata gtgtaggaat aactcgtttt ttacgatcca ttgaagaatt tgcgccatca
     2701 gcggtgagat gagtttttga cggctgtaag cgctcacgag tgaccctaag cccagataat
     2761 catagcttaa ggtgaggatt tctgagggat taattaggaa aagtaataat taatttcttg
     2821 tcagtcggcg ttatttgtta ggacaatatt tcaaaaataa tccagcttgt cgtcaataat
     2881 tgcgataaag agataaatta aagttaaata attaaatcat taaaaaatta cgccacaaaa
     2941 atgcgaggcg tcttcaggat tagaatcgat aaaacagaat gggttaaaga gagcgatata
     3001 atagcggcgg gtacttgagg ctatctgtct caggcattag ctgaacggca gatagagaaa
     3061 agccccgagt gatattttac tatcaacccg aggcccccta tatgctgaac acatgtagag
     3121 tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat aaggcgatga
     3181 ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta acgagaaaag
     3241 acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc acggcttacg
     3301 aatccgagta agtgcaacgg cggggagtga tccccgccac ttttcaggtg ctcgcatatc
     3361 ttcaacgcac cctaatttta cccaagcctg cccacgggca ggcttttggg gaggttagcg
     3421 ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc gcgggctgtt
     3481 gtgctcaccg gttgggccac cgaagctttc ttgcagctct tgcagcagct gtttctgctt
     3541 ctcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac cctgtgcgcc
     3601 accgcggaca gacttgacgc ctttaccgcg catacggaaa agcttaccgg tctgggtttc
     3661 gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt cgccacccag
     3721 cgccgccata gcgaagttga tcgggacttc gcaatagagg ttgttgcctt cacgctcgaa
     3781 aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg cgccatgctc
     3841 gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc ctgccgggat
     3901 tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt tgttgcacgg
     3961 atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta cagcgaagaa
     4021 tccctgacgc atctgcacct gaccagaacc atgacaggtc ggacaggtct gcggctgtgt
     4081 acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg gaatgcggat
     4141 ctctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat agcgtaaatc
     4201 agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc cgaaaacgtc
     4261 accaaaaata tcgctgaagt ctgcgccgcc gccaaaaccg ccgccgccca taccaccttg
     4321 ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt cggtcagaac
     4381 ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac cctggttacg
     4441 gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ttgatttcac gctcttccgc
     4501 tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt ttaaattgcc
     4561 cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt ataattaccc
     4621 gtttataggg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg acaacatcgt
     4681 catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg gcatgttgct
     4741 gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ctcctgcatt ttcgcttcga
     4801 tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagtgca gactcgatag
     4861 cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta cgggtgctgt
     4921 gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta cggtcagctt
     4981 cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc agaccagaag
     5041 aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg gaaacgtgca
     5101 ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg cgcggtgccg
     5161 ggttgatacc atccaggttg aactgaccca gagatttgtt atcagccgca cgtttacgtt
     5221 caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta gagaacacct
     5281 ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc acaccgccca
     5341 tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct ttcacgtcac
     5401 cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc gggttaacgt
     5461 ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt ggcatacgag
     5521 tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca gcgtcctgca
     5581 gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg ctttccagtt
     5641 tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcatctgca gtgatgtacg
     5701 gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt tctgccgctt
     5761 ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga tctttcttga
     5821 attcttcaac caggtagttg atcagacggc tgtcgaagtc ttcaccaccc aggtgggtat
     5881 caccgttggt agccagaact tcgaaggttt tttcgccgtc aacttcgtcg atttcgataa
     5941 tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg ttgccagtac
     6001 ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata cgttttactt
     6061 ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg ttaaagtatg
     6121 ccggtacggt gataacagct tcagttaccg gttcacccaa gtaatcttca gcggttttct
     6181 tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg cctttaactt
     6241 cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg gaaacatcac
     6301 gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca aacagggtgt
     6361 tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt tcaccatcct
     6421 gggtataggc aataatagaa ggcgtggtgc gatcgccttc ggcgttctcc agcacgcgag
     6481 gagtggtgcc atccataatc gctacacaag agttggtagt acccaggtcg ataccaatta
     6541 ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt gagtctgtaa
     6601 taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac tcactcactg
     6661 cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc aaaaattttt
     6721 tgtgcttttg cgcagaaatt tcgcgatata gcaggacaag acgtgaaact gacagcaatt
     6781 ttattcatat aaagaatgaa tcgcagccaa gacattcatc aaaaaattgt aataaaaaga
     6841 aaacattacg cacctgaatc ttctctttat cagcagtaaa ctagtgggta tccatccccc
     6901 tacctctccc cactaagaga atccttatga aatccgtttt tacgatttcc gccagcctgg
     6961 cgattagtct gatgctgtgc tgcacggcgc aggcaaacga ccataaaatc ctcggcgtca
     7021 ttgcgatgcc acgtaacgaa accaacgatc tggcgctgaa acttcctgtt tgccgaattg
     7081 ttaaacgcat acaactctct gccgaccatg gcgatttaca gttaagcggt gcatcggttt
     7141 atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa ataaaagaag
     7201 ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc tgcgtctcaa
     7261 aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg aaaattatcg
     7321 gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta cctgaatgat
     7381 tcagatctgg attttctcca gcattgcagt gaggaacagt tggcaaattt cgcccgattg
     7441 ctcacccata atgaaaaagg caaaactcgc ctctccagcg tactgatgcg caacgaactg
     7501 tttaaatcta tggaagggca tcccgagcaa catcgccgca actggcagct gattgctgga
     7561 gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca cggtaaattg
     7621 tatcgggcca ttttgctcga tgtttcaaaa cgattgaagc tgaaagccga caaagagatg
     7681 tctacgtttg agattgagca acagttactg gaacaatttc tgcgtaacac ctggaagaat
     7741 atggacgagg aacataaaca ggagtttctg cacgcggtcg atgcgagggt gaatgagctg
     7801 gcagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggcgt gtcgcatttg
     7861 ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt acttgggcat
     7921 ggtttgctgc gcggcgcggg gctgggaggc cctgtaggtg cggcactaaa tggggttaaa
     7981 gcggtcagcg gcagcgccta tcgcgtgacg attccagccg tactgcaaat cgcctgcctg
     8041 cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat tttcattttc
     8101 ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc cctgaaaaca
     8161 ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt gaggaattat
     8221 gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg gcatgaccac
     8281 cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta ttcttgccat
     8341 gggcattttc tacggcggca tcgcgcaaat ttttgccggt ctgctggagt acaaaaaagg
     8401 caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga cgttggttgc
     8461 gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt tccttggtgt
     8521 ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcacgc tgaaaggcgc
     8581 acgcgttctg caattcgttt tctttagcct gactgtgctg tttgccctgc tggcgatcgg
     8641 taacattgcc ggtaacgccg caatcatcca ctttgccggc tggattggtc tgatctgtgg
     8701 tgccagtgca atctatctgg cgatgggtga agtactgaac gagcagtttg gtcgcaccgt
     8761 tctgccgatt ggtgaatccc actaattgtt ttgagatccc cccgctcggg gggatttttt
     8821 tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac ttctggtgcc
     8881 gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc aaaaataccg
     8941 tgtaccacaa cgttacccgc agcgtccttc acaccttcca gcgtctcttt aatagacttc
     9001 ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacaccaac ctgacgcgaa
     9061 aggatcgcag ttggtacaaa atgcaggctt atctggcgca tctgttcacc aaagccaggc
     9121 atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg cgccgggcca
     9181 gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccactagctc acacaacgtt
     9241 tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa cggcgtggtt
     9301 agcgccgatg tcagccattc ttccagcgca gggatgcctt tatcctgata aacgccgctg
     9361 gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa gatttttccg
     9421 tttaaacagt ctcgttaaac agaatgatac actgcgaagg gagtgacaga caggcttcag
     9481 aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag atcgccgatc
     9541 attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc cagtttatct
     9601 actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat acgcgccgga
     9661 cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc gctctcagcc
     9721 agctctttca gcagtgtagg tgcgatggta agacggtcgc agcctgccag ttccagaatt
     9781 tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg ctctttgtag
     9841 tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc gtactctttc
     9901 ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga gatcaggaac
     9961 acgcccgctt ccgcacaagc acgagcctga gcgaaggaga acagcagggt caggttacag
    10021 ttgatgcctt ctttttccag ctgttctgca gcacggatac cctgccaggt agaagccagt
    10081 ttgatcagaa tacgatcgtt gctaatacca gcatcgttgt agagtttgat caggcgtttt
    10141 gctttcgcaa ttgacgcttc ggtgtcatag gaaagacgcg catcaacttc agttgagata
    10201 cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt gtcggtcgcg
    10261 tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac agcatcatca
    10321 atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg gttggttgtg
    10381 gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc cactacggtg
    10441 gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt taaacagctt
    10501 gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca gcgcaaccgc
    10561 cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc gttgatatcg
    10621 cccgccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag tacctcgtag
    10681 cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc gaccggcaat
    10741 aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt ggggatcggt
    10801 aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg gatttgtgca
    10861 gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc cacagccagg
    10921 cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg gtagcggcaa
    10981 cctggccggc gttgcgctgg caattaccgc cggtggacct ggagccgtct tctggatgtg
    11041 ggttgccgcg tttatcggca tggcgacctc gtttgccgaa tgttccctcg cacagcttta
    11101 taaagaacgt gatgccaatg ggcagtttcg tggcggaccg gcatggtata tggcgcgcgg
    11161 gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg cctacggcat
    11221 aattttcagc ggaattcagg cgaacgccgt tgcgcgagcc ctgagttttt cttttgattt
    11281 tcccccgctg gtgacaggta ttattctcgc tgtctttgct ctgctggcga tcactcgcgg
    11341 tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga taatctgggt
    11401 actgaccagc ctggtgattt gcgtaatgaa tatcgggcaa cttccccacg tcatttggtc
    11461 tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg gatatacctt
    11521 aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg
    11581 gtcgacgcca aacgcggcag cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg
    11641 gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat
    11701 gctgatatta ctggcgggta acggcacaac ctacatgccg ctggaaggta ttcagcttat
    11761 ccagaaggcg atgcgggtgc taatgggttc ctggggtgct gagtttgtta ccctcgtggt
    11821 tattctgttt gccttcagct ccatcgttgc caactacatt tacgccgaaa acaatctctt
    11881 ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac
    11941 ggtcatcggc ggcaccttgc taagtcttcc gctgatgtgg caactggcag atatcataat
    12001 ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac
    12061 cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt
    12121 gcgttatccg gatatcggcc gccagctttc tccggacgcg tgggatgatg tttcgcagga
    12181 gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct
    12241 gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc
    12301 gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc agttgatcca
    12361 tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact
    12421 ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc cggagaatgc
    12481 ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacctt
    12541 cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg
    12601 cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga
    12661 gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa
    12721 cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt
    12781 taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga
    12841 gaagaacggc aaatttaaga taatcagctt ctacgctaag aaagcacgcg gtctgatgag
    12901 tcgttttatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga
    12961 aggttacttc tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga
    13021 gcagcgttaa gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa
    13081 agcatgcaga ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg
    13141 catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc
    13201 tggaccatga ccgccgtgat gatcatgatg agctttctta tggtgacgcg gcggtggcgg
    13261 cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg
    13321 gtcgcgccag tgaccgccat cccagtaata accgcgatta tcacgatcgc ctatctgtaa
    13381 ttttactgac gggactaacg taatttccgc agcctgcgct gccatgggag cgaccagaac
    13441 cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg
    13501 cccgttatgg gtcgatatcc ggagattacg cgatgagaac ggcggttgtt attctccgca
    13561 atcctaatct ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttctc
    13621 tccatatttt cttcacaaaa aagccgggct gcataaaagc aaacccggcc tgattgagat
    13681 aatgaataga tgttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg
    13741 cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa
    13801 cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag
    13861 gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg ctacggcagc
    13921 gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc
    13981 atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc
    14041 ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg
    14101 agtcgctttg ggggaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt
    14161 ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt
    14221 caaatcgccg aagtttccgc ttggtaccga aacaaccagc tggttgcgcg cctcctgcgg
    14281 cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat
    14341 cgagttagct gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt
    14401 caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc
    14461 caatgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac
    14521 cactttcaca ttcggcaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga
    14581 ggtcgcggtc agaatggtca ctggcttatc acccgcaata tgggtcagca tttgtgccat
    14641 aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc
    14701 gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc
    14761 ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg
    14821 ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc
    14881 atgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct
    14941 gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc
    15001 gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt
    15061 acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacacagag cgaacaaggt
    15121 cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc
    15181 ctgccggaag cctggcagta atcgttcacg gtagggttca gcgataacat ctttcatcag
    15241 cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagcctg ccagatgtcg
    15301 cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc
    15361 cgtcgagact ttaatccccg gatacgccag cacccacagc cactcatcaa acccaggcac
    15421 ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccta gaaaacacgg
    15481 tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc ccatcaaagc
    15541 cagcaaacga gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca tcagcgccgc
    15601 gaccaccgaa caggcgctgg agcctaagcc cgaaccgatc ggcatattct tttccagagt
    15661 catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc actgataaac
    15721 gatattttcc cgtggttctg acggcagctt atcggcaaag cgcccgaggt tgttgagact
    15781 gaatatctct gccgcctcaa ccgtaactac atctccgagc aatgcaccat caacgggtgt
    15841 caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata
    15901 aactttaacc atgtcagact cctaacttcc atgagagggt acgcagcaga tcggcaaaga
    15961 caccagcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct
    16021 gataatagtg gctatagaag gccagggcgt tttcgccatt tttcactttg aacagcggat
    16081 cattaccatc cacttcggca atcttcacgc gacagacgcc atcttcatca atattgccaa
    16141 catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagcgatcgt
    16201 cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg
    16261 gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt tcacgggcga
    16321 gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcgga tccggttcgg
    16381 tataacccat ttcccgtgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact
    16441 tgccgaagat ataagaaagc gatcctgaaa gaatgccgga gaacttcatc aattcatcac
    16501 cagcattgag cagattttgc aggttttcaa taaccggtaa tccagcccca acgttggtgt
    16561 catagaggaa tttccgccgc gatttttccg ccgcatgacg caacagatgg tagtaatcca
    16621 tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt
    16681 cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca
    16741 gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgcg
    16801 ccagttcttc ctgccagttt tccagattta ggccatgcac attggtgagc agtgccttcg
    16861 agttggcaac accgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgttgac
    16921 gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga
    16981 taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat
    17041 cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaata gcgacaatgt
    17101 tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtacgca
    17161 taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc ggctccagta
    17221 agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagcc cgcacacagt
    17281 cgctttgcgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca
    17341 cggaaatacg ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt
    17401 tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga
    17461 ccggtaattc gtcttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc
    17521 cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt
    17581 gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca
    17641 acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc
    17701 aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac
    17761 cgttgcgtcc aagtaccacc agttcgcctt tttcattacc ggcggtgaag cctgccatca
    17821 gcaccatgtg atcagccgga atacgacttg ccgcaatacg gcgggtggac tcagcaatat
    17881 cgacggtaga ttcgaggtaa tgccccactg ccagtagttt ttcgaccgga tcgataacgg
    17941 taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc
    18001 cacggcaaat cagcgcagcg ttaatgctat ccgggcactg ccctaacaaa ctaatgccat
    18061 gcaggacatg ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg
    18121 ggaatcccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat
    18181 cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt
    18241 ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc
    18301 tttccagaat atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga
    18361 acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa agcccgcact
    18421 gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg
    18481 taatggtgat ggtggtgata atggtggtgc taatgcgttt catggatgtt gtgtactctg
    18541 taatttttat ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc
    18601 aataaaattt taatttactc acggcaggta accagttcag aagctgctgt cagagactct
    18661 ttttttaatc cacacagaga catattgccc attgcagtca gaatgaaaag ctgaaaaata
    18721 cttactaagg cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca
    18781 acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt
    18841 ttatgtcatc tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt
    18901 gcagttgatg ttggtctgcc gttgtgtcac ttttcgtcgg ttgttgtatt aatgttgcta
    18961 attgatagca atagaccatc actgcctgcc ccagattgag cgaaggataa tccgccacca
    19021 tcggcacacc agtaagaacg tcagccaacg ctaactcttc gttagtcaac ccggaatctt
    19081 cgcgaccaaa caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca
    19141 ccagttcaac tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga
    19201 cagtgaaatc gacatcgtgt aacgattcaa ccagtgtcgg gaaaacttta atattatcaa
    19261 taatatcacc agatccatgt gcgacccagc gagtggctgg ctccagatgt gcctgactat
    19321 cgacaatccg cagttcgcta aaccccatcg ttttcattgc ccgcgccgct gccccaatat
    19381 tttctgctct ggcgggtgcg accagaataa tcgttatacg catattgcca ctcttcttga
    19441 ccaaatgacc gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat
    19501 ccttaacgta agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc
    19561 tgactgtact aacggttgaa ttgttaaaaa atgctacata tccttctgtt tacttaggat
    19621 aattttataa aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc
    19681 aactagttgg attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat
    19741 caaactgtta acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg
    19801 gtgtccggcc cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt
    19861 ttgacactgt cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt
    19921 tgatggaaag tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt
    19981 aatttgcagc atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg
    20041 gcaatttagg tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta
    20101 acacgcaaca cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca
    20161 gatggcgcgg aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat
    20221 atcaatctgc cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat
    20281 gttgcgttga tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa
    20341 atcggtgcag atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca
    20401 cgcaacctgc tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt
    20461 gaaagctaca agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct
    20521 gatggcgagc agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa
    20581 aacccaggca aaattcagtc ccgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg
    20641 aaaccacacg accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct
    20701 acgccggata cgccggaaat catcgccacc atccacggtg aaggttatcg cttctgcggt
    20761 gatctggaag attaatcggc gttaccaccg tcaaaaaaaa tggcgctttt tagcgccgtt
    20821 tttatttttc aaccttattt ccagatacgt aattgatcgt ccattgtaac ttctttactg
    20881 gctttcattt tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg
    20941 ccggccagcg ccaccaccag cacactggtt cccaacaaca gcgcgctatc ggcagagttg
    21001 agcagtcccc acatcacacc atccagcaac aacagcgcga gggtaaacaa catgctgttg
    21061 cgccagcctt tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg
    21121 gcgattatcc atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa
    21181 aacatcacca atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg
    21241 agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta
    21301 gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg
    21361 gggaagtttt cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca
    21421 aaccagctgc tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggc
    21481 aaaaaatcac ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta
    21541 cgcccggcag gcaccacaga aagatcgccg gtaccgctta aattcagggc catattcagc
    21601 ttcaggttct gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct
    21661 tgctctaacc cggtgccggg ttcaatggtc agcgccgttc cgttaacttc aggcgctttc
    21721 accacaccaa taccacgcgc atccccgacg ctaatcacaa taaagggctt gcctaagatg
    21781 atatttggcg cgttgagctc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa
    21841 tcactgtgcc agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg
    21901 ccatcaacca tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg
    21961 gttttatcct cttcctgcac cgtataaagc tcggtcacag gaatagcgat aagcggccca
    22021 acgagttttt gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa
    22081 tcggcacgtt cgacaatcac ttgccgaatc agcattatcg gaatcaacaa cagcaatact
    22141 gcaccaaaca aggtggtcat tttccagaac aggggggatt tcaacatagt cttctccttt
    22201 gcaatggcag caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg
    22261 tgaagtcgaa gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg
    22321 ttaaacaaac gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca
    22381 tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata
    22441 ccactaccgg tatccagcac cttaagggcg acgtgttcct gatccacttc ggcgcttagc
    22501 gttatgcgac cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc
    22561 tgctccagta acgccggttc agcagcaaca ttaacctcag tgggcataac atgtagagtg
    22621 atgttttttt ctgccaactg cacggtgcgc gcttcgctga cgcggcgaaa taatgccgcc
    22681 acatcaacaa cagtcagaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac
    22741 gtttctacca atgcctgcat acgcgcattt tgcgtcagaa tgttgtcggt aaaacgagcc
    22801 accacttccg gtggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt
    22861 gggcttttta gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc
    22921 agcttcacgc gcatactttc cagcgcctgc gcaagcttac gcaactcgct actgccgagt
    22981 tcaggaagag gaacgggctt attgtcagtg acggaatcgg catagcgagt gagccgggca
    23041 atggagcggt tgatccacca aaccatgcct gcgccaatca ccagtgcaat ccccagcaaa
    23101 atggcgctgg cccataaaat tcgccgctcg ctacgcttaa tgaccggagc catcgccgcg
    23161 ttcggtttgc ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca
    23221 acatacatca ccgaactttc gggatcggca ggattttgca acgtgctgcg cgcaccatac
    23281 tgaccacgca acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta
    23341 tttgccgaat cgaacaatac tttaccctgc gaatcggtca tatagacatg atattcattg
    23401 cgcactttgt taatgccacc gatattggcg cgaaacgggc gatgttgtag ctgattaaac
    23461 gcctgcgcca gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc
    23521 gccagcaacg ttgcggtatc gattaacgtc ccctccgttg ctcttcgcac gcccggttta
    23581 acttctttga caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag
    23641 cccagcagca accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc
    23701 gatgagtatt aatcggtgaa agatcggggt tgatggcgcg cagcttggca cgcaacgttt
    23761 taatgtgggt atcaatggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca
    23821 ttagttgctg gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa
    23881 actcataccg cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat
    23941 tcaattcaaa atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac
    24001 gcagtaaggt gcgcaccctt gcgcacactt cgcggggtga aaacggttta gccacatagt
    24061 cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga
    24121 acagtacagg taacgccgga tggagcgcca gtaactggcg gcataattca aagccgctga
    24181 tatcaggcaa accaacatcg agaatcatga cgtcgggagc ctgctggcga gctttatcca
    24241 gcaccggcaa gcctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga
    24301 ccagcgtgtc ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa
    24361 atcgcctctg ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg
    24421 aaccttctac aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat
    24481 aaaagcgcac gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct
    24541 cgccctgagc tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac
    24601 aagaaatagc cgcatcggag gtatcttccg ccagacccaa tcccccttta atacccccgg
    24661 ttttcgcccg gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca
    24721 caacaatttt gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctcct
    24781 ctgcatgagc caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt
    24841 tcatattgtt accattttta tctattacac gagcaattag ttcgtcacgg taaataacat
    24901 attgattaag aacaaaatct caatactttt atttccgtca agccctaaaa catactctac
    24961 taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt
    25021 caggtgctcg ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga
    25081 ccttttaatc tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc
    25141 gaaagcaggt tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc
    25201 tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac
    25261 tgcgcgtccg attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac
    25321 ccgcgcattc aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg
    25381 gagcgccttt ggtattcgcc cgccgctacg tctgggtgaa ttcactatgc cagagcacaa
    25441 atttgtcacc ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct
    25501 ggagcaaatc tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg
    25561 caacgcgccg accattccgc cggtgctcta cggcctgaat gaaacgcgtc cgagtcagga
    25621 taaagacgac gaacaagaag tattctatac caccgcgtta gcccaggatc aggcagatgg
    25681 ctatgtactg acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta
    25741 tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg gtatatggaa catgcatgcc
    25801 aatgcttaac ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga
    25861 tgccaaagca ggagatcgcc caattaatct gcgctgtgaa ctgctgattc cgatccgtcg
    25921 ttaacgctgc aactcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc
    25981 aacaacccag ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca
    26041 gttacgcaga cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag
    26101 gcatcccagt gcaataccat gactcaccag cagcggtcgg cttccctgcg gtaagtcccg
    26161 gcaagattcc agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg
    26221 aatacgcccg tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt
    26281 cagagaatcg atatgtcttt tttccagcac acccatgttt aattcacgca ggcgagaatc
    26341 aaagatgatg tcgcagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc
    26401 taaatcgcta ctgatgatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat
    26461 cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc
    26521 ggcgttccac tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg
    26581 tatactgctg taacgttaat tttctgagta atgctgatta tgcaccaagt tgtctgtgcg
    26641 accaccaatc ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa
    26701 ggatcctgcc atattgcatc cgttgccgtc gagagcggtg taccggaaca accctttggc
    26761 agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca
    26821 gaggctgatt tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg
    26881 gtggtgattg aaaacgccag ccagcgcggc gaggcgcgtt cggctacttt gccgttgcct
    26941 gcggtgattc tggaaaaagt gcgtgaaggc gaggcgctcg gccccgtgat gtcgcgttat
    27001 accggtattg atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa
    27061 ctcacccgcg ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcataat
    27121 gccgtgtatc aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc
    27181 acgatgcctg atgcgacgct gccgcg
//
LOCUS       DAZFWL020000055        26590 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFWL020000055 DAZFWL020000000
VERSION     DAZFWL020000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.055"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            39..1361
                     /locus_tag="ACHKNP_RS12325"
                     /old_locus_tag="pgaptmp_002465"
     CDS             39..1361
                     /locus_tag="ACHKNP_RS12325"
                     /old_locus_tag="pgaptmp_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGU4704617.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFDLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            1389..1700
                     /locus_tag="ACHKNP_RS12330"
                     /old_locus_tag="pgaptmp_002466"
     CDS             1389..1700
                     /locus_tag="ACHKNP_RS12330"
                     /old_locus_tag="pgaptmp_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGU4704618.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            1756..3429
                     /locus_tag="ACHKNP_RS12335"
                     /old_locus_tag="pgaptmp_002467"
     CDS             1756..3429
                     /locus_tag="ACHKNP_RS12335"
                     /old_locus_tag="pgaptmp_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HGU4704619.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            3454..4893
                     /locus_tag="ACHKNP_RS12340"
                     /old_locus_tag="pgaptmp_002468"
     CDS             3454..4893
                     /locus_tag="ACHKNP_RS12340"
                     /old_locus_tag="pgaptmp_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGU4704620.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(4950..5168)
                     /gene="marB"
                     /locus_tag="ACHKNP_RS12345"
                     /old_locus_tag="pgaptmp_002469"
     CDS             complement(4950..5168)
                     /gene="marB"
                     /locus_tag="ACHKNP_RS12345"
                     /old_locus_tag="pgaptmp_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HGU4704621.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(5200..5583)
                     /gene="marA"
                     /locus_tag="ACHKNP_RS12350"
                     /old_locus_tag="pgaptmp_002470"
     CDS             complement(5200..5583)
                     /gene="marA"
                     /locus_tag="ACHKNP_RS12350"
                     /old_locus_tag="pgaptmp_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HGU4704622.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(5603..6037)
                     /gene="marR"
                     /locus_tag="ACHKNP_RS12355"
                     /old_locus_tag="pgaptmp_002471"
     CDS             complement(5603..6037)
                     /gene="marR"
                     /locus_tag="ACHKNP_RS12355"
                     /old_locus_tag="pgaptmp_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HGU4704623.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            6249..6914
                     /gene="marC"
                     /locus_tag="ACHKNP_RS12360"
                     /old_locus_tag="pgaptmp_002472"
     CDS             6249..6914
                     /gene="marC"
                     /locus_tag="ACHKNP_RS12360"
                     /old_locus_tag="pgaptmp_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HGU4704624.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(6939..8129)
                     /gene="ydeA"
                     /locus_tag="ACHKNP_RS12365"
                     /old_locus_tag="pgaptmp_002473"
     CDS             complement(6939..8129)
                     /gene="ydeA"
                     /locus_tag="ACHKNP_RS12365"
                     /old_locus_tag="pgaptmp_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HGU4704625.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8342..9393)
                     /gene="yneK"
                     /locus_tag="ACHKNP_RS12370"
                     /old_locus_tag="pgaptmp_002474"
                     /pseudo
     CDS             complement(8342..9393)
                     /gene="yneK"
                     /locus_tag="ACHKNP_RS12370"
                     /old_locus_tag="pgaptmp_002474"
                     /inference="COORDINATES: protein motif:HMM:NF033404.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            complement(9471..10352)
                     /gene="yneJ"
                     /locus_tag="ACHKNP_RS12375"
                     /old_locus_tag="pgaptmp_002475"
     CDS             complement(9471..10352)
                     /gene="yneJ"
                     /locus_tag="ACHKNP_RS12375"
                     /old_locus_tag="pgaptmp_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4704626.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            10453..11841
                     /gene="sad"
                     /locus_tag="ACHKNP_RS12380"
                     /old_locus_tag="pgaptmp_002476"
     CDS             10453..11841
                     /gene="sad"
                     /locus_tag="ACHKNP_RS12380"
                     /old_locus_tag="pgaptmp_002476"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HGU4704627.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            11905..12831
                     /gene="glsB"
                     /locus_tag="ACHKNP_RS12385"
                     /old_locus_tag="pgaptmp_002477"
     CDS             11905..12831
                     /gene="glsB"
                     /locus_tag="ACHKNP_RS12385"
                     /old_locus_tag="pgaptmp_002477"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HGU4704628.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            12831..13190
                     /gene="yneG"
                     /locus_tag="ACHKNP_RS12390"
                     /old_locus_tag="pgaptmp_002478"
     CDS             12831..13190
                     /gene="yneG"
                     /locus_tag="ACHKNP_RS12390"
                     /old_locus_tag="pgaptmp_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HGU4704629.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            13329..14747
                     /locus_tag="ACHKNP_RS12395"
                     /old_locus_tag="pgaptmp_002479"
     CDS             13329..14747
                     /locus_tag="ACHKNP_RS12395"
                     /old_locus_tag="pgaptmp_002479"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU4704630.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            14974..16425
                     /gene="uxaB"
                     /locus_tag="ACHKNP_RS12400"
                     /old_locus_tag="pgaptmp_002480"
     CDS             14974..16425
                     /gene="uxaB"
                     /locus_tag="ACHKNP_RS12400"
                     /old_locus_tag="pgaptmp_002480"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HGU4704631.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLARQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            16632..17546
                     /gene="yneE"
                     /locus_tag="ACHKNP_RS12405"
                     /old_locus_tag="pgaptmp_002481"
     CDS             16632..17546
                     /gene="yneE"
                     /locus_tag="ACHKNP_RS12405"
                     /old_locus_tag="pgaptmp_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HGU4704632.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDIQFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(17550..18044)
                     /locus_tag="ACHKNP_RS12410"
                     /old_locus_tag="pgaptmp_002482"
                     /pseudo
     CDS             complement(17550..18044)
                     /locus_tag="ACHKNP_RS12410"
                     /old_locus_tag="pgaptmp_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(18043..18879)
                     /gene="lsrF"
                     /locus_tag="ACHKNP_RS12415"
                     /old_locus_tag="pgaptmp_002483"
                     /pseudo
     CDS             complement(18043..18879)
                     /gene="lsrF"
                     /locus_tag="ACHKNP_RS12415"
                     /old_locus_tag="pgaptmp_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462959.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
     gene            complement(18906..19928)
                     /gene="lsrB"
                     /locus_tag="ACHKNP_RS12420"
                     /old_locus_tag="pgaptmp_002484"
     CDS             complement(18906..19928)
                     /gene="lsrB"
                     /locus_tag="ACHKNP_RS12420"
                     /old_locus_tag="pgaptmp_002484"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HGU4704633.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            complement(19940..20932)
                     /gene="lsrD"
                     /locus_tag="ACHKNP_RS12425"
                     /old_locus_tag="pgaptmp_002485"
     CDS             complement(19940..20932)
                     /gene="lsrD"
                     /locus_tag="ACHKNP_RS12425"
                     /old_locus_tag="pgaptmp_002485"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HGU4704634.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            complement(20932..21960)
                     /gene="lsrC"
                     /locus_tag="ACHKNP_RS12430"
                     /old_locus_tag="pgaptmp_002486"
     CDS             complement(20932..21960)
                     /gene="lsrC"
                     /locus_tag="ACHKNP_RS12430"
                     /old_locus_tag="pgaptmp_002486"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HGU4704635.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            complement(21954..23489)
                     /gene="lsrA"
                     /locus_tag="ACHKNP_RS12435"
                     /old_locus_tag="pgaptmp_002487"
     CDS             complement(21954..23489)
                     /gene="lsrA"
                     /locus_tag="ACHKNP_RS12435"
                     /old_locus_tag="pgaptmp_002487"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HGU4704636.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLEALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASPKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETFYGLRTLRSGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIS
                     AQNVAVLLISSDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            23738..24691
                     /gene="lsrR"
                     /locus_tag="ACHKNP_RS12440"
                     /old_locus_tag="pgaptmp_002488"
     CDS             23738..24691
                     /gene="lsrR"
                     /locus_tag="ACHKNP_RS12440"
                     /old_locus_tag="pgaptmp_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HGU4704637.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            24770..26360
                     /gene="lsrK"
                     /locus_tag="ACHKNP_RS12445"
                     /old_locus_tag="pgaptmp_002489"
                     /pseudo
     CDS             24770..26360
                     /gene="lsrK"
                     /locus_tag="ACHKNP_RS12445"
                     /old_locus_tag="pgaptmp_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
BASE COUNT         6785 a         6269 c         6643 g         6893 t
ORIGIN      
        1 tgggatgtat aaatatccaa aacaacaggt ataaaaaaat gggattaatg gcctcattcg
       61 aacgtggaat ggaacgtttt cttgttccag ttgctatcaa gttaaactca caaaaacatg
      121 ttgcagcggt gagagatgga ttcgttttta cgtttccaat tatcatggca agttcattaa
      181 ttatattaat taactttgcc atattatcgc cagacggttt tattgccgga ttactgcatc
      241 tgaatagcat tttccccaac cttgaaaaag cacaagctat ttttactccg gtaatgaatg
      301 gttctgtaaa tatcatgtca attatgattg ctttcctggt cgccaggaat atggcgatta
      361 gctatgagca agatgatctt ttatgcggat taacggcaat aggagcattt tttattgttt
      421 atacgccata tcagatgata gatgggcaag cattcctgac gaccaaatat ctcggcgcgc
      481 aggggttgtt tgttgctgtt atcgttgcat tgatcaccag tgaaatattt tgtcgcttag
      541 ctcgaaaccc aaaaatcacc ataacgatgc cggcagctgt accgcctgcg gtagcgcgtt
      601 catttaaagt tttattgcca atattttttg tcatggtgtt cttttccgca cttaattatt
      661 gcctgacact gatatccccg gcaggactaa acgatcttat ttacacatta atccagacgc
      721 cgctcaaaca tatgggaacg aatatctttg cggtaattat cctgggggct gtgggtaatt
      781 tcctgtgggt gctggggatc cacggaccta ataccacatc ggcaattcga gaaaccgttt
      841 tttctgaggc taatctggag aatctctcct gggccgctca acatggcact acctggggcg
      901 cgccatatcc gattacctgg acttctatta atgatgcatt cgccaactgc ggcggttcag
      961 gtatgacgtt ggggttattg ttggctattt ttatcgcttc taagcgtgcg gaataccgtg
     1021 atctggcaaa aatgtcattt atccccggta ttttcaatat caatgaaccg ataatgtttg
     1081 accttcctat tgtacttaac cccatcatga tggtgccgtt tattatggtt cccattgtta
     1141 actgtgccat tggttacttc tttgtttcga tggaaattat tccaccggtt gcttatgccg
     1201 tgccctggac tacgcccgga cctttaattg ctttcctcgg aaccgggggg aactggctgg
     1261 ctttactggt tggtttttta tgtttaggtg tggcgacaat gatctattta ccttttgtta
     1321 ttgccgccaa caaagtcaat aacatgacaa ctaacggata attccattta cccaggaagg
     1381 agattgtgat gtttgcagat gaagaacttg ttatggagtt actggtcaac gcggggcagg
     1441 cacgttctga cgcgatggaa gccatacgtt gtgccgggca aaaagactgg cagggcgcaa
     1501 ctcagctaat ggccagttct gaatctgcct gtcttcaggc acataagatt cagaccgcat
     1561 taattagtca ggatgaaggc tgtggcaaaa taaaagttaa tttaattctg attcatgcac
     1621 aggatcattt gatgaatgca atattatgcc aggatttagc cagggaaatt atttcattaa
     1681 gaaaagaact ccatgcctga ttttttcctc cagtgcgata gctggaggaa attattatac
     1741 tcttaaggat aagtcatgaa tataaagacg cttaacgtca gccttttgtc tttttctatt
     1801 attacagcat tgtttccatt gaacgcgatg gcaacaaaat taaccataga gcagcgcctt
     1861 gaattgcttg aaaatgaatt gtcgcaaaat aaacaagagc tgaaagcaac acagaatgaa
     1921 ctgggagtat ataaatcccg actttcgacc ttacaaaaaa gcatcacgga aaacaaatat
     1981 aaatcagcct cgcttgctga aatatcagcg acatctcccg tcgctgataa catcaaaaat
     2041 gaaaacggtg aacagaactc gtttaccgca gcgcatacta taaatggatc gcagcaagtt
     2101 gccgttattg aaagtaaagg cgacaaaacc actatcgaaa gcgtgaccct gaaagatatc
     2161 agtaaatata taaaggatga tattgggttc agctatcagg ggtacttccg ctcaggctgg
     2221 ggtactggta atcacggctc accacaaact tatgcagcgg gttctctggg acgttttggt
     2281 aatgagatga gcggttggtt tgacctgacc ttaaatcagc gtgtttataa tcaggacggt
     2341 aaaacggcaa atgcggtcgt tacctatgat ggcaacgtgg gtgagcagta taacgatgcc
     2401 tggtttggtg acagtgccaa tgaaaatatc atgcagttca gcgatattta cctgacaacg
     2461 cgaggttttt tacccttcgc accagaggca gacttctggg tcggcaaaca taaactcccg
     2521 caatatgaga tccagatgct ggactggaaa accttaacca cggatgtcgc cgcgggtgtg
     2581 gggattgaaa actgggcact tggtgtaggg ctgtttgata tgtccttaag ccgtgatgat
     2641 gtcgatgttt actctcgtga tttttcgcgt accagccaga tgaataccaa ttctgtggat
     2701 gttcgttatc gcaatatccc gttatgggat gatgcgacat tatcattaat ggctaaatat
     2761 tccgcaccta ataaaacgga tcaacaacaa gataacgaaa atgacaacag ttattttgaa
     2821 atgaaagata gctggatgct gacttctgtt ttacggcaaa acctgcaacg cgatacgttt
     2881 aatgaattta cgttacaggt tgccaataat tcctatgcca gcagttttgc cagtttctca
     2941 gatgccagta atacgatggc gcatggtcgc tattactatg gtgatcatac caatgggatc
     3001 gcctggcgtt taatctctca gggcgagatg tatcttactg acaatattat tatggctaac
     3061 gcgcttgtct attctcacgg cgaagatgtt tatagttatg aaagtggtgc tcatagtgat
     3121 tttgacagta ttcgcactgt aataagaccg gcctggatct ggaatacatg gaatcagacg
     3181 gggcttgaat taggctggtt taagcaacag aacaaaactc agcagggtgt aacactaaat
     3241 gaatcggctt ataaaacgac actctggcat gcattgaaag tgggtgaaag cattttaggt
     3301 tcacgaccag aaattcgctt ctatggaacg tatatcaata ttctggataa cgaattatcg
     3361 aattttaagt ttaatgagaa cagcaaagac gagtttatgg ccggcatcca ggcggaagtc
     3421 tggtggtaat ctattttagt taaggagttg tgtatgtcag gatttaaaaa aggtttttta
     3481 tggggcggtg cggtagccgc gcatcagttg gaaggtggct ggaatgaagg aggaaaaggc
     3541 atcagcatcg ctgatgtgat gactgctggc gctcacgggg tgccgcgtga agtgacagaa
     3601 ggcgttatcg acgggcttaa ttatcccaat catgaagcaa ttgattttta tcatcgctat
     3661 aaaacagata ttcagttatt tgccgagatg ggattcaaat gctttcgaac ttccattgcc
     3721 tggacacgaa tctttccgca aggtgacgaa caggagccga atgaagaggg tttacaattt
     3781 tatgatgatc tgttcgatga atgccttaag cagggaatgg aacctgtggt gacgctttca
     3841 cattttgaga tgccttatca tctggtgaca aaatatggtg gctggcgaaa ccgtaaactg
     3901 atcgactttt ttatccgctt cgcatcaacg gtcttcacgc gctataaaga aaaagtaaag
     3961 tactggatga cgtttaacga aatcaataat caggtgaatt tcagcgaaag cctgtgtcca
     4021 tttactaatt ccggtatctt gtattcgcca gaggaagata tcaatgagcg cgaacaaata
     4081 atgtaccagg cggtacatta cgagttagtt gccagtgccc tggcggtaca gactggaaaa
     4141 tcgatcaatc ctgaatttaa cattggctgt atgatcgcca tgtgtcccat ctatcctctg
     4201 acgtgtgcac ccaacgatat gatgatggcc acgaaagcaa tgcatcgtcg ttactggttt
     4261 actgatgttc atgctcgtgg atattatccg caacatatgc tgaattactt tgccaggaaa
     4321 ggattcaacc tcgatatcac accagaagat aacgcgattc ttgccagtgg ttgtgtcgac
     4381 tttatcggct ttagctacta catgtctttt acgacacaat tttcgcccga taacccgcaa
     4441 ctggattatg ttgaaccacg agatttggtc agcaaccctt atatcgatac atccgaatgg
     4501 ggatggcaaa ttgatccggc agggctacgt tattcactca actggttctg ggatcatttc
     4561 cagttgccgc tgtttattgt cgaaaatgga tttggtgcgg ttgaccagag acaagctgac
     4621 ggcacggtga acgatcacta tcgcattgat tactttgctt cccatattcg ggaaatgaaa
     4681 aaagccgtag ttgaagatgg tgttgactta attggctaca ccccgtgggg ctgcattgac
     4741 ctggtttctg ccggaacagg ggaaatgaaa aagcgctacg gaatgattta tgtcgataaa
     4801 gacaacgaag ggaagggaac gctggaacgg atacgtaaag cgtcgtttta ctggtatcgg
     4861 gatctcatcg ccaacaatgg cgaaaatatt tgagacttga ggaagatatt acggagatgt
     4921 caggctctgt gatcagagcc tgacaataac tacatagcgt gttgattata atagggcacg
     4981 ccgagcgcat ccgacttatc actgccagta cccatgtgat ttaaatcaaa cggtggttgt
     5041 tcctgcgatg ggggaacaac cacgacattg gcacaagaag taacaactgg ctgcgtggtt
     5101 tgttccgcaa cgccctgcgc ggaaaagaga ataagcgcag ctgctattgc ggatgaaagt
     5161 ggtttcatga ttgcctcagt gacgttgtca cgttatcaac tagctgttgt aatgatttaa
     5221 tggatgtaaa aagcgcgatt cgccctgcat attggtcatc cggtatttat gcggcggaac
     5281 atcaaagtaa tttttgaagg ttcgggtcag agtttgttgc gactcgaagc catatcgttc
     5341 tgccagatag agtatcggct cgttactttc cttcagcttt tgcgcgattt ccgtcatctt
     5401 acggctgcgg atgtattggc ctaatgaatg accggtttct tttttaaaca tccgttgcag
     5461 gtgccattta gagtaacctg aacgctctga cactttctcc agtgacagtg gcgattccag
     5521 gttgtcctcg atccagtcca aaatgctatg aatggtaata gcgtcagtat tgcgtctgga
     5581 catcgtcata cctctttttt gtttacggca ggactttctt aagcaaatgc tcaagtgttg
     5641 ccacttcgtc cgccgtcagg ttttttgtta attcttgatg caggtcctgg ccaactaatt
     5701 gatggcattg ttcgcatatt gccgcgccgc tggtggtaag ttttaccagt acgccgcgct
     5761 tatcattcgg gttcggcaac ctttcaaccc agcctttaca gaccaggcga tccagcatac
     5821 gggtcagtgc tccaaggtcg acagacaaca cttttttcag ttcaaccgga gtaatacacg
     5881 ccgcgcagcg gatagagcag agcaccttaa actgtgccgc ggtaatatcc agcggagaca
     5941 gatactcgtt aagcagacga tctttcttct gattaaccat atggattaag cgacccaatg
     6001 gaataatttc attgaacaga tcgctggtac ttttcacatt agttgccctg gcaagtaatt
     6061 agttgcaggg gataatattg cccaggcaag tataagtcaa ataaatgaat tgaccgatgc
     6121 cacgttttgc taaatcggtt caagttttgg tcaatgcgat gcaaggttag ctaaagagtg
     6181 acactttatc cttgtttacc ccttatactt ttcgctgata acccagatac acaggataac
     6241 aaccaccaat gttagatttg tttaaagcaa ttggcctggg gctggtggtg ttactgccgt
     6301 tagctaaccc attaacaact gtcgcgttat ttcttggcct ggcaggcaac atgaacagtg
     6361 ccgaacgtaa tcgtcagtcg ttgatggcct cggtgtacgt atttgccatc atgatggtgg
     6421 cgtattacgc cgggcaactg gtgatggata catttggcat ttcgattccc ggtctgcgaa
     6481 ttgcaggcgg cttaatcgtc gcgtttatcg gttttcggat gctctttccg caacagaaag
     6541 cgattgattc accggaggcg aaaagcaagt cggaagagct ggaagatgaa cccagtgcca
     6601 atattgcctt tgtgccgcta gcaatgccaa gtaccgctgg tccgggaacc atagcgatga
     6661 ttattagctc ggcgtccaca gtgcgtcaga gttcaacctt cgctgattgg gtactgatgg
     6721 ttgcgccgcc gctgatcttt ttcctggtgg cggttatttt gtggggaagc ctacgcagct
     6781 ccggcgcaat tatgcggctg gtgggcaagg gtggcattga agcgatctcc cgtctgatgg
     6841 gattcctgct ggtatgtatg ggtgtgcagt ttattattaa cggcatcctg gaaatcatta
     6901 aaacgtacca ttaaaaaagg cccgaatggg cctttcaact attgcgtctg ttcttcgagt
     6961 gtcactggcc agcggcgaaa tataatgatt gaccaaatta acgcggcaaa agcaggcacc
     7021 gcgcccacat aaccaatcat cgacattgac cagtgcaaac tcacctgatt acctaccaac
     7081 gcacccgccc cgattccaat attaaatatc ccggagaata gcgccatcgc gacgtcggta
     7141 gcatctggtg ccagcgccag cactttaacc tgcataccaa gcccgatgat catcatcgcg
     7201 atcccccaga aaatactcag caccccgagg tgtatttcac tgttcgccgc aggtaacagc
     7261 aatgccaggc acaccagcaa cagcgcaatc gccgtactca ccaacgcaga cgcatactga
     7321 ttacccagtt taccgaaaat cacgctgcca ataatgcccg caccaccgag taataacagt
     7381 aatgccgtgg caaagttggc gctgaatccc gcaatgtttt gtacaaaagg ctcgatatag
     7441 ctgtatgccg tgtaatgggc ggtgacaacc accacagtta acaaataaat gctcatcaat
     7501 gccgggcggc ggaacaatag cgggaggctt ttcagtgaac cggaatgctc actgggcagt
     7561 aagggaagta acttaatcag gcacaaaagg gtgataagcg ccccaatacc aatcgcgaag
     7621 aaggtcattc gccaaccgaa atactggccc acaatgcgcc cgagaggtaa acctaagacc
     7681 atcgccagtg ctgtaccggt ggcaattaaa ctcaatgcct gtgctcgctt cccggccgga
     7741 gccatacgga tcgccagaga cgccgtaatc gaccagaaaa tcgcatgtgc aaaagccaca
     7801 ccaatgcgac tgatcaccag aacggtaaag ctccacgata aaaacgacag tacgtggctg
     7861 gcaataaaca ccacaaacag gcagatcagt aatttgcgcc gttcaacctg gctggtcatt
     7921 aacataaaag gcaatgacat tagcgccact acccatgcat aaatggtcaa cataatgccg
     7981 acctgagcgg tttgcatgtg aaaactttgc gcaatgtcag agagcaggcc aacagggaca
     8041 aattcggtgg tgttgaagat gaaggcggcg actgccagcg taacgacccg tagccacgcc
     8101 actttgcggg aaacagtgtt tgttgtcata acagattatc gggtgagttg cgaaaaagtg
     8161 atgaaatgat cttaaaacgg ttaatgatgt gaatacaatg catttgtgat ctggatcgcg
     8221 ttttccgttt ctctgacgct gattctgttc cgcgtcagag agtcggtaac atcacggttt
     8281 aatgacgttc agggaggtct ggcgttttgg cggttgcggg tataggtggt ctgacagggg
     8341 agcgaggcct gtttttcacc aggtcttcgt gagtaacttt tggcggcgtt tcatttttta
     8401 caaacagttt gagggtcctg cgcgccatat gtttgataat tttatcatca cttaatgatg
     8461 ctggcatcaa cacgtcattc agatcacttt cattgggatt cataaattgg tgatagtgca
     8521 ataacatatt ggcgaaaaat aaaaaaaagt cctgaaaatg aggagattct tgttctgcta
     8581 aaagggataa aaagcagatg cagacttcgg gtagtacatg tttgttagaa cctaatgaat
     8641 ccatcaggct ttgcaataaa tcctctttta attcgccgtc tgttatgtcg agaatggcgg
     8701 tgatcaagtt aggatcttta ttgacgaacc atctgtgaac ctcagagtta ctccataatc
     8761 gggggagtat gacaaaatta ccgcgagaga taaaattcag gacaagcaaa tcaaactggc
     8821 gtaagtaatc tgcagcggta tggcgatccg gaggcagggg ttcaatatgt ggtgtatgcg
     8881 ctcttttgat gggaacgcca aggttgatca ttggaaattg ttcaataaaa tcattcagaa
     8941 acagaaatag cacacgctcg acgccggtct gaaatacgcg ccccatagtg acaggctttg
     9001 ggttgggagc gcaaaaatat taatttctcc atcatggcga aaattatcta aataaatagg
     9061 gcttaaattt attactacct gaaattcaat attttctttg tgtcttccat tcttgacggg
     9121 ataagtgagt ttaaactgaa ccttttcatt gtatagcgtg tcgttacccc gaaaagaagt
     9181 cctctgaata tatgataagt tatcaaggtt attaaatatc ttttttgtgg ctgagttgat
     9241 gtttaaagac gtgcgccact cttcatcaca tattattgta cttagcgcat caataaagtc
     9301 ctttaaaact tcttttacct gaggtgcaat attacgtacg ggaaaatcat caggaagaga
     9361 tatgtaatta ctgatgctta ctggcgtaac catatttgcc ccatatagtc ataagtcatc
     9421 ggaacatcac tatatagggc atttgagctg caagaactat caaaaatagc tcattggtaa
     9481 ccctgttttg ccgagtcagg cacatccagt agttgaataa atgcctcaag cggacgcgtt
     9541 ttcgcaccgc gacgccagac cagccaggtt gttaaccaac gccattgctc agctaacggc
     9601 cacgcttcaa cctggtgatg ccccggcata ctttccagca tagagcgtgg aataagcgca
     9661 atgcctgctc ctgcgaccac acaggccaac atcccgtgat acgactccat ctcgtgaata
     9721 gttcccggag cggcaccgtc agcatgaaac cagctctcga agtggcgacg atacgaacaa
     9781 ttggcgcgga aggcataaat gttactgccg ttaacctgac tggcgcggat tactggcgca
     9841 tgaccttgtg gcgtgacgat catcagctcc tcgcggtata ccggtatccc gtcaatggca
     9901 gtatggttaa tgggcccatc aataaatgcc gcattcagtt ttccctccag cactccttcc
     9961 agcatagtgc cggaagggcc ggtggaaagt gaaaactgaa ttttgggata acgatggttg
    10021 aattccgcca gcgtggccgg aatgcgcact gctgcggtgc tttccagcga accaagagaa
    10081 aataaacctt gcggctcatc gccagcgaca acgctccgcg cttcatccac taacgcgaga
    10141 atttgttggc tatagcgtaa aaagttatgc ccggcaggag agagacgtaa acgctgattc
    10201 tcgcgaataa acagatctac ccccagttct gtttccagct ggcgtagacg ggtagtgaga
    10261 ttcgacggta cgcgatgcac ttttgctgca gcctgggtaa tgctgccagc ttcggcaacc
    10321 gcgttgaaca tctccagttg ggtcagatcc ataacattct cgattcgtga ataagtggct
    10381 taatattatt cattttaaag caagagtaaa tctgcgtatc ttcataccat gactcataaa
    10441 ggagataccc cgatgaccat tactccggca actcatgcaa tttcaataaa tcctgccacg
    10501 ggtgaacaac tttctgtgct gccgtgggct ggcgctaacg atatcgaaaa cgcacttcag
    10561 ctggcggcag caggctttcg cgactggcgc gagacaaata tagattatcg tgctgaaaaa
    10621 ctgcgtggta tcggtaaggc tctgcgcgcc cgtagcgaag aaatggcgca aatgatcacc
    10681 cgtgaaatgg gcaaaccaat caatcaggcg cgcgctgaag tggcgaaatc ggcgaatttg
    10741 tgtgactggt atgcagaaca tggtccggca atgctgaagg cggaacctac gctggtggaa
    10801 aatcagcagg cagttattga gtatcgaccg ttggggacga ttctggcgat tatgccgtgg
    10861 aactttccgt tatggcaggt gatgcgtggc gcggttccca tcattcttgc aggtaacggc
    10921 tacttactta aacatgcgcc gaatgtgatg ggctgtgctc agctcattgc ccaggtgttt
    10981 aaagatgcgg gaatcccgca aggcgtatat ggctggctga atgccgacaa cgacggtgtc
    11041 agtcaaatga ttaaagattc gcgcattgct gctgtcacgg tgaccggaag tgttcgtgcg
    11101 ggagcggcta ttggcgcaca ggctggagcg gcactgaaaa aatgcgtact ggaactgggc
    11161 ggttcggatc catttattgt gcttaacgat gccgatctgg aactggcggt taaagcggcg
    11221 gtagccggac gttatcagaa taccggacag gtttgtgcag cggcaaaacg ctttattatc
    11281 gaagagggaa ttgcttctgc atttaccgaa cgttttgtgg cagctgcggc agccttgaaa
    11341 atgggcgatc cccgtgatga agagaatgct ctcggaccaa tggctcgttt tgatttacgt
    11401 gatgagctgc atcatcaggt ggagaaaacc ctggcgcagg gtgcgcgttt gttactgggc
    11461 ggggaaaaga tggctggggc aggtaactac tatccgccaa cggttctggc gaatgttacc
    11521 ccagaaatga ccgcgtttcg ggaagaaatg tttggccctg ttgcggcaat caccgttgcg
    11581 aaagatgcag aacatgcgct ggaactggct aatgatagtg agttcggcct ttcagcgacc
    11641 atttttacca ccgacgaaac acaggccaga cagatggcgg cacgtctgga atgcggtggg
    11701 gtgtttatca atggttattg tgccagcgac gcgcgagtgg cctttggtgg cgtgaaaaag
    11761 agtggctttg gtcgtgagct ttcccatttc ggcttacacg aattctgtaa tatccagacg
    11821 gtgtggaaag accggatctg acgcgacata ttcagctctg atatactcgc aggtcttttc
    11881 agacctgcgg tccaggagta gaaagtggca gtcgccatgg ataatgcaat tttagaaaac
    11941 atcttgcggc aagtgcggcc gctcattggt cagggtaaag tcgcggatta tattccggcg
    12001 ctggctacag tagacggttc ccgattgggc attgctatct gtaccgttga cggacagctt
    12061 tttcaggccg gagacgcgca agaacgtttt tccattcagt ctatttccaa agtgctgagt
    12121 ctcgttgtcg ccatgcgtca ttactccgaa gaggaaatct ggcaacgcgt cggcaaagat
    12181 ccgtctggat caccgttcaa ttctttagtg caactggaaa tggagcaggg tattccgcgt
    12241 aatccgttta ttaatgctgg tgcgctggtg gtttgcgata tgttgcaagg gcgattaagc
    12301 gcaccacggc aacgtatgct ggaagtcgtg cgcggcttaa gcggtgtgtc tgatatttca
    12361 tacgatacgg tggtagcgcg ttccgaattt gaacattccg cgcgaaatgc ggctatcgcc
    12421 tggctgatga agtcgtttgg caatttccat catgatgtga caaccgttct gcaaaactac
    12481 tttcattact gcgctctgaa aatgagctgt gtagagctgg ccaggacgtt tgtctttctg
    12541 gctaatcagg ggaaagctat tcatattgat gaacctgtgg tgacgccaat gcaggcgcgg
    12601 caaattaacg cgctgatggc gaccagtggt atgtaccaga acgcggggga gtttgcctgg
    12661 cgggtggggc taccggcgaa atctggcgtt ggtggcggta ttgtggcgat tgttccgcat
    12721 gaaatggcca tcgctgtctg gagtccggaa ctggatgatg caggtaactc gcttgcgggt
    12781 attgccgtcc ttgaacaatt gacgaaacag ttagggcgtt cggtttatta atgcagtctc
    12841 tcgatccact cttcgcgcgt ttatcccgtt caaaatttcg ctctcgcttt cgtctgggca
    12901 tgaaagagcg tcagtattgc ctggagaaag gcgcgccagt catcgaacaa catgcggcgg
    12961 attttgtcgc taaacgtctt gctccggcat taccggctaa tgacggtaag caaaccccca
    13021 tgcgcgggca tccggtgttt atcgctcagc acgccaccgc gacctgctgt cgcggttgtc
    13081 tggctaagtg gcataacatt ccgcagggtg tttcgttaag cgaagaacaa caacgttata
    13141 tcgtggcggt aatctatcac tggctggttg tacagatgaa tcagccatga gaattgcgga
    13201 aaacgataaa atagcattcc cagggtacct ttatttgccg ccgctgttag cgaacatgct
    13261 atcctttatg agaatttact tattacgttg agataattgc cacccgcaat tgtatggaca
    13321 gataattaat gcatgtacac cccatttcaa ctttccgttt gttccaggaa ggtcatctgc
    13381 tacgtaatag catcgctatt tttgcgctaa ccacgctgtt ctattttatt ggtgcagagt
    13441 tacggctggt tcacgaactt tctcttttct ggccgctgaa tggcgtaatg gcgggggtgt
    13501 ttgcccgcta tgtctggctt aatcgactgc attactatgc gatcagttat gtggcgatgc
    13561 tggtttatga tgccataacc accgaatggg gactggtttc actggttatc aatttctcca
    13621 atatgatgtt tattgttacc gtcgccttac tggtcgtgcg ggataagcgt ctggggaaaa
    13681 ataagtatga gccagtaagt gccttacggc tatttaatta ctgtctgatt gccgcattat
    13741 tatgcgctat tgtcggggcg attggttcgg tcagtattga tagtctggat ttctggcctt
    13801 tgcttgcgga ctggttcagt gagcaattct caacgggcgt gttgatcgtg ccttgtatgc
    13861 tgacgttggc tattcctgga gtgctgccgc gctttaaagc agaacagatg atgcccgcca
    13921 tcgcgcttat tgtgtcggtt attgcctcgg tagtcattgg cggagcgggg agtctggcgt
    13981 tcccgctgcc tgcattaatc tggtgtgcag tgcgctatac gccgcaggta acatgtctgt
    14041 tgacctttgt caccggtgcg gtggaaatcg tactggtggc aaattcggtg attgatatct
    14101 cggtcggttc gccgttctcc attccggaaa tgttctccgc acgtctcggt attgccacga
    14161 tggcgatatg cccaattatg gtttctttta gcgtggcagc gatcaattcg ctaatgaagc
    14221 aagttgcgct gcgagccgac tttgattttc tgactcaggt ttactcacgg tccggtcttt
    14281 atgaggcgtt gaaaagtcca tcgctgaaac agacacaaca tctgacggtc atgctgcttg
    14341 atatcgacta tttcaaaagc attaacgata actatggaca tgaatgtggc gataaagtgt
    14401 taagcgtgtt tgcccggcat attcagaaga ttgtcggtga taaggggctg gtggcgcgca
    14461 tgggcggcga agaatttgct gttgcagtgc cgtcggtgaa tcctgtcgat ggtctgctaa
    14521 tggcggaaaa aatccgtaaa ggcgttgaac tgcaaccatt cacctggcaa caaaaaacgc
    14581 tttatctcac ggtaagtatt ggcgtcggta gtggtcgcgc atcgtaccga acgctgaccg
    14641 atgattttaa taaattgatg gtcgaagccg atacgtgtct gtatcgctcg aagaaagatg
    14701 ggcgcaaccg taccagcact atgcgttacg gtgaggaagt tgtctgacat ctttattcat
    14761 tccgcgcgat ttgaatgcaa tgtgttctac cactttatga tccaggaaaa ggcgtatttc
    14821 ctcgattaaa ccatgatccg cgccacactt ttctcataaa actgctcatt aatcatcagg
    14881 agttgatcaa aacaggggtt cggcgtatta aagtttgcta acctgtcgta ccaattcaaa
    14941 caggttgtat gactaatcag aagggaaccc attgtgaaaa cactaaatcg tcgcgatttt
    15001 cccggtgcac agtatccaga acgtatcatt cagtttggtg aaggtaactt cctgcgcgcc
    15061 tttgttgact ggcaaatcga tctcctgaat gagcacaccg atctgaattc tggtgtggtc
    15121 gttgttcgtc cgattgaaac ttcattccca ccgtcactga gcacgcagga tggtctgtat
    15181 accaccatta tccgtggcct gaatgagaag ggggaagcgg tcagcgacgc gcgtctgatt
    15241 cgctcggtta atcgtgaaat cagcgtctac agtgaatacg atgaattcct gaaactggct
    15301 cacaacccgg aaatgcgctt tgttttctct aataccaccg aagcgggtat cagctaccat
    15361 gcgggcgata aattcgatga cgcgccagcg gtaagttatc cggcaaaact gacccgtctg
    15421 ctgttcgaac gttttagcca tttcaacggt gcgctggata aaggttggat catcatcccg
    15481 tgtgagttga tcgactacaa tggcgacgcg ttgcgtgaac tggtgctgcg ctatgcgcaa
    15541 gagtgggcac tgccagaagc atttattcaa tggctggatc aggctaacag cttctgttct
    15601 acgctggtgg accgtatcgt taccggttat ccgcgcgatg aagtggctaa actggaagaa
    15661 gagctgggtt atcacgatgg ttttctcgac accgctgaac acttttacct gtttgtgatt
    15721 cagggaccga aatccttagc gactgaactg cgtctggaca aatatccgct caacgtgctg
    15781 attgttgacg atattaaacc gtacaaagaa cgcaaagtgg caatcctcaa cggtgcgcac
    15841 accgcgctgg tgccggtggc gttccaggca gggctggata ccgtaggtga agcgatgaac
    15901 gatgctgaaa tttgcgcatt cgttgaaaaa gctatctacg aagaaattat tccggtactg
    15961 gatttgcctc gtgatgaact ggaatctttc gccagtgcag tcaccgggcg tttccgtaac
    16021 ccgtacatta agcatcagct gctgtctatc gcgctcaacg gtatgaccaa gttccgcact
    16081 cgcatcctgc cgcagctgct ggcacggcag aaggcaaaag gcacacttcc ggcgcgcctt
    16141 actttcgcat tagcggcatt gattgccttc tatcgtggtg agcgtaacgg agaaacatat
    16201 ccggtacagg acgatgcaca ctggctggaa cgttatcagc aactctggag ccagcatcgt
    16261 gaccgtgtta tcggtaccca ggaactggta gcgattgtgt tggcagaaaa agaccactgg
    16321 gagcaggacc tgacgcaagt gcctggtctg gtcgagcagg ttgctaatga cctggatgcg
    16381 attcttgaaa aagggatgcg cgaagctgta cgtccgttgt gctaataaca ccgttacttc
    16441 tgaacccggt ttcgaccggg tttcttattt cactatatta gttacaacat cctatgtgcc
    16501 tttatcatta acagatgcca catgcagcgc ctgttaatgt tgtaagcttg taggtaattt
    16561 gtaagtagcg tcttgagaat gtgatcgtaa tcacgtttaa tagggcgact ttttcacgcc
    16621 tgaagataac catgattgtt cgtccacaac aacactggct gcgccgtatt tttgtctggc
    16681 acggctcagt attatccaag atatcatcgc gcttactact caattttctc ttttctatcg
    16741 ctgttatttt catgctgccc tggtacacgc atctgggcat caaattcacc ctcgcaccgt
    16801 tcagcattct cggtgtcgcc atcgccattt ttcttggttt tcgtaataat gccgggtacg
    16861 cccgttacgt tgaagcgcga aaactttggg ggcagttgat gattgcctca cggtcgttac
    16921 tgcgcgaggt aaaaacgaca ttgccggatt cggcaagtgt aagggagttt gcccggttgc
    16981 aaatcgcctt cgcccactgt ttacgcatga cattacgcaa acagccacag gcggaagtgc
    17041 tggctcatta tctcaagact gaagatcttc agcctgtact ggcttcgaac tctccagcta
    17101 accgtatctt gttaataatg ggggagtggt tggcggttca gcgccgcaat gggcagcttt
    17161 cagatatcca gtttattagc ctcaacgatc ggcttaatga tatttcagcg gttctggcag
    17221 gctgtgagcg cattgcctac acgccaattc cctttgccta caccctgatt ttgcatcgta
    17281 ccgtttatct gttttgtatc atgctgccgt tcgcgctggt cgtggacctg cattacatga
    17341 cgccttttat ctctgtgctg atttcctaca cttttatttc gctggattgt ctggcggaag
    17401 aactggaaga tccgttcggt actgaaaaca atgatttacc gctggatgcc atctgcaacg
    17461 ctattgaaat tgacctgtta cagatgaacg atgaagcaga aattccagca aaaattcttc
    17521 ccgatcgtca ttaccagctg acgtgataat tactccgtac ggcgggcaac aataaacaga
    17581 cgcggaaatg ccagcagtat ctgtccgttc tcttgcagtg gatactgctc ttccagcatc
    17641 tgatggtagc gcgtaagaaa aagctgctgt tcgctctcgg tcagatcctg taaccacgga
    17701 cgtaatccag tggcggtcac ccaatcgata atcgcctggt gcgacggcat ctggtgatag
    17761 taagtggttc gccagatatc gacctcacat ccggcttcgc tcaaaatatc gtagtaagca
    17821 tgaacgccag ccaacggctc acgcccgcga tccgggtagt tttgttccca ggcaacttcg
    17881 cgcatgagca catgggtcgg ctccagccag ttatctggca tctgtactgc cagcacgccc
    17941 tgcggattaa gtaaagaaac cagatgagga aacaattcgt agtggtcggg cagccattgc
    18001 agtgaggcat tagcaaaaat cagatcgagt gcctgttccg gttgccgttt cgttatggtg
    18061 aaccaccgcc tgtacggctt tcatcatcgc caccggatgg tcagactgga aaatattacg
    18121 ccccatatcc acaccagaag cgccctgatc gatagcctgc cagcacattt ccagcgcctc
    18181 gcgctccggt aattttttac cgccagcaat aacaatgggt accggacatc cggcaacaat
    18241 ccgttcaaaa cctttttcga cataataggt tttgataatt tgcgccccca tttcagcggc
    18301 gattcgagtc gcgagcgaga aataacgctg atcgcgcacc atatctttgc ccacgccagt
    18361 cacggccatg gtcggcattc ccactttcat tccggcatca accagctgaa taatattttt
    18421 gatcgactga tgttcatatt cgctgccgat ataaacctgc gccgccaccg cgcaactgtt
    18481 caggcgcacg gcatcatcca tcgataacgc cacggcttca ttacttaatt ccgccagaat
    18541 agagttcgct cctgacgccc gcagtaccac cggcttattg gtcgctgggg gaactacgct
    18601 gcgcaaaatg ccgcgcgtac acattaatac atcggcatgt tcaaacagcg gggcgatatt
    18661 tatatcaatg cgttcaagtc cggtagtcgg cccctgaaaa taaccatggt caaaggccag
    18721 catcacggtt ttacccgttt tcggattaaa tatccgcgat aagcgtgact gcattcccca
    18781 atccagcgca ccgcaacctt tcaggataaa agggatattt tgttgcgatt gatcggtacg
    18841 aaaatcttta ccatctttaa tatcgtctaa atctgccata acttactccg gttaagtaat
    18901 gcacatcaga aatcgtattt gccgatattc tctttgttga atatcacgcg ctccggtaac
    18961 agtacgatgc cattaccatc cgcttcgtag tcatagccct gaacgctgtt tggcgagact
    19021 tccacctgac ctacgccctg gatatccagc ttgtcgcccg ttttcattga tccttttttc
    19081 aataatgcat ccgcgacata cactgaaatt ttgccttgct gaaccacatc ccacaggcca
    19141 aattctttca ccgtgccgcg ttccacatat ggacgcatca cgtttggcgt actgaatccg
    19201 acaatcgcaa ctttgtcatt tttcaggttt tctgcggctt gtgcggcagc gggcagggcg
    19261 ttggcgtcgg gggcgataat ggcgtcgaga tcgctatacg cttttaatat tccttctgcg
    19321 gtttgtaatg atttagtggc atcgttatag ccaaactgcg tagtgacaat ttcccagcca
    19381 ggatgctctt tggcgatttt cgctttcgct tctttcaccc actggttttg gtccgtaacg
    19441 gtggggcttg agtagaaaaa cgcgactttg gctttgtctt tattcacctg acgcgccgcc
    19501 atatccacca acatacctcc caactgggcg ggcgttccct gattaatgta gtaagagcgg
    19561 cactccggtt tagtatcaga gtcccaggtc agcactctca caccacgttg catggcgcgt
    19621 ttcagtgccg gacacaagcc atcaggcgaa accgcagaaa cgataatggc gttataacct
    19681 tgattgacga agttattaat caactgtacc tgaccagaaa cactgggttc tgtcggcccg
    19741 tcgtaggtca catcaacgcc cagctcttta cccgcttgtt gtgcgccgtt gccaccgctg
    19801 gtaaaaaatc ccacgccaac cagtttggga ataaatgcaa tacgctctgc ggcctgcaca
    19861 ttcatagaga ttgcggcaat gccaagagcg ctaagtaagg cgattttctt aaagcgatga
    19921 agtgtcatga agatatcctt tatggcaatg ggttattggc ccgacgcgcc agccactctt
    19981 taatttgctg gcgatgcagg ctaacggaac gacctacgac aacgacgata agtagcgcac
    20041 cggaaagggc gctggacacc tgatttggca ctcctgccat ttgcaaacct tgctgcaaat
    20101 atcccactaa taaaaccgca atggcggtgc cgataatgga accggaacca ccataaatat
    20161 tggccccgcc aagcaccacg gcggtgatgg cgggcatcag aaacgacgca ccgagatcgg
    20221 aacgtgctga accaaaatac gataccagca gcacagcggc gaccgcagac gccagccccg
    20281 tcatggcata gagcgcacat aaggtgcggt taactggaat cgcgctataa agcgccacgc
    20341 gcgggctttg cccaatcaaa aacacattac gtccggcatg ggttttatgc agccagagcc
    20401 agaaaacgag gagacatatc aggaagataa tcagcggaac ggggagtccc agcacatcca
    20461 ggttagcgaa atctgtaaac gccatcggga atccaccaat accttcgtac cccgtcgctc
    20521 cggccatacc ggaaagcagc agagcgcttc cggcaaacag atacagcgtg ccaagcgtaa
    20581 tcaccagcgg gttaactttg gtatagataa ttaatccggc gttgatcagc ccgcacaatg
    20641 cgccgagcag taaggtcagg agtatcgcca gcggcatcgg cacaccactt tgaaacagta
    20701 cgcccaatgc aatggcgcag aggccgatgg tcgaaccaaa cgaaatatcg atcccgccac
    20761 tgacaatcac catcgtcagc ggtagggcga caatgccaat gcagataaag tcactggtgc
    20821 tgaacagcaa catattgaga tctaacattc gcgggttaat tgcaccaaat gcgacaatct
    20881 caataacgag cagtgcggca agagccagtt cccaaccgta gcgaatacgc attatgcggc
    20941 ctcccgtttt ttacctgacg aagcgggttt aacggatggc ggtggcgtca taaagcgggc
    21001 atatttttgc cgccgtagat tacgttccag cgcacaacgc aggcgtccat caaacaccag
    21061 caccgccagc agcacgaggc ccgcgataaa atcgttccac catgccggaa tgcgcaacag
    21121 caccagtacg ctatcgatct gcgtcaggaa ccatgcgccg agtaccgcac cgatgatcgc
    21181 accggaaccg ccgagcaaac taatgccgcc cagcacgcag gctgcaattg ctttcatctc
    21241 cagcccggta ccggtctggt tggggataaa accaatctgc gaagcaaaca caattcccgc
    21301 cagtgccgcc atgcagccgt tcaacgaaaa tgccacaatg cgaatggctt ccgtacgaac
    21361 acccagttga cgagcgccct gtaaattatc gcccgtggca taaaaactgc gtccaaacgc
    21421 cgtctttgcc agcagccagg ccataaatgc caccagaatt atcgtcaacc aaccaatcgc
    21481 ggaaacgcca aacagcagcg gtgcggagag ctgtttcagt tcggcgggta acccttcaat
    21541 ccatttaccg ccagtccaca gcaacatgat gcctctgtac aaccctaacg tgccaagggt
    21601 ggcaacaatg gcagggatct ttagccacgc gaccaggaca ccgttgaaaa atcccgcgag
    21661 caaaccaagc agtaaagtcg cgacacaggc aacaggtagt gaatatcctg cgttcagtaa
    21721 catccccaac agcaccgcgc acattccggt aatcgaaccc actgaaacat caatattgcg
    21781 cgtaagcatt accagtgtcg cgcccattgc cagcaggatc aggatttgcg cgctgctata
    21841 aaccatggtc agcgtttgca cacttaaata ctgacggtcg agaaaaccgg gtaatacaaa
    21901 cagcagcacc accgccagca atgccgtgat ttcacggttg ttctgaataa acttcagcat
    21961 gatgcctcct gacgctgact atcgccgaag gcaacgcgca taatagtctc gacattaata
    22021 tcgcgcccgg tcagtgcaga gtgggtaatt tcgccctgat gcatcacata cacacgatct
    22081 gtcatcagtt cgatctcttc cagatcagag gagataagca gcacagccac attctgtgcg
    22141 gagatgctgc gcaacagctg gtagatatca ttacgggccg agacatccac gccgcgcgtc
    22201 ggctcatcga caatcaatgc ttgcggcgaa gcttccaggc atttggcaat gaggattttt
    22261 tgctggttgc caccggataa tgtccgtgca gcttgttccg gttggttaaa tttaatattc
    22321 agcgcccgac gataacgttc cagggtggca ttatctttcg cggttttcgc ccagaatcca
    22381 cgaaggttat gagtaagggc gcagacgttc caggccagcg aagcatcgag attcagtccg
    22441 gatgactggc gatcttccgg cagataaacc agaccgcgca gtaaacgttc tccagtggat
    22501 aatttattga tctctttacc attcagcata atgcgtccgc tacgcaaagt acgcagacca
    22561 tagaacgtct cggccagttc tgtgcgtccg gcccccacca gcccagccag gcccagaatt
    22621 tctccggcat tgagcgtcat gctgacattc ctgaaacctt cgccggtcag attttccagt
    22681 gtcagcaccg gcgttccggc agcatgttgt gggcggttac caggtaactc cagccataat
    22741 tttgggctgg cagagagcga tttttcccgt accactggcg tgatggcctg aataatgtcg
    22801 tcggtagaca gttcgctggt tttgccgctt aaggcgatgg ttccgtcgcg catcacgcta
    22861 attcgatcgg caatctggcg aatttccggc agcttatgcg agataaaaac aatacccacg
    22921 ccagtagcaa gcagctcttg caagcgagta aacaagcgtt cggtttccgc aggggtaagc
    22981 gaggcggtag gttcatcgag gatcagaatc cgcgagtcgc gcattagccc gcggaggatt
    23041 tccaccattt ggcgatcggc gacatccagc gatcctgcca gactatgcag atcaaactgg
    23101 cagcccagcg cctccagcaa gttcttcatt ttttgcatgg agagctgttt ttttgccatc
    23161 ccaaacagga tgttttcttt tatcgacagg cttgggaaaa gcagcggttc ctggggaacg
    23221 agataaatgc ccagctgatg agcatgaact ggcgttaatc tggcgtagtt gttgccccca
    23281 atctccagcg taccgctatc agcaggggta ataccggcaa taatcttcat taacgtcgat
    23341 ttaccggcac cattgccgcc gagcagggcg tggacctccc cctgatgcaa cgtaaaatcg
    23401 atgcctttca ggacattgac ccctgaatac tgtttataaa ccgagcgggc gcaaagtagc
    23461 ggtaacgcgc gggtatcact cgtttgcata tttcccccgt tcagttttgc aggtgagttt
    23521 tgaacaaatg tatttctgct tttaatttgt tcataacctt aggtggacat tgcacatatt
    23581 tccgacgaat agatcacaat ttatgctatt ttgattttca cggttgcgtt tgttcatgct
    23641 cgtagagtca aactgtggct gccatcacag atataaatgc gcaagaactg aacaattgca
    23701 ttaaagattt aaatatgttc aaagtgaaga atgaattatg acaatcaacg attcggcaat
    23761 ttcagaacag ggaatgtgtg aagaagaaca ggtcgcgcgg atcgcgtggt tttactatca
    23821 cgacgggctg acccagagcg agatcagcga tcgtctcggc ctgacccgtt tgaaagtgtc
    23881 gcgcttgctg gagaaagggc atcagtccgg cattattcgc gtacagatta attctcgctt
    23941 tgaaggctgt ctggaatatg aaactcaatt acgtcgtcag ttttcgctgc aacatgtccg
    24001 ggtgatccct gggcttgcgg atgctgatgt cggcgggcga ctagggatag gcgcggcgca
    24061 tatgttgatg agtttacttc aaccacaaca gatgctggcg attggttttg gcgaggcaac
    24121 catgaatacg ctgcaacgct taagtggttt tatttcgtca cagcaaattc gcctggtcac
    24181 gctctccggt ggcgtcggtt cttatatgac gggtatcggg cagcttaacg cggcgtgcag
    24241 tgtgaatatt attccggctc cgttgcgggc atcctccgct gacattgccc gtacgctaaa
    24301 aaatgaaaat tgcgtcaaag atgttctgtt agccgcgcaa gcagcggatg tggcgattgt
    24361 cggcattggg gccgtcagtc aacaggacga cgcgacgatc attcgctccg gttatatcag
    24421 ccagggcgaa cagttaatga ttggtcgaaa aggggcggtt ggcgacattt taggctactt
    24481 ttttgatgca aaaggtgacg ttgtcacgga tattaaaata cataacgaac tgattggctt
    24541 acctttaagc gcgctgaaga ccatacccgt ccgggttggc gtggcagggg gagaaaataa
    24601 agccgaagca attgccgctg caatgaaagg cggttatatc aacgcactgg ttaccgatca
    24661 ggatacagca gcggcgattt tacgtagtta aatttgcatg accttattac cgggaatgat
    24721 ggagaacgta tagaggaaca gacagaacgc ataagccgag gataatctaa tggctcgact
    24781 ctttacccct tcagaatcaa agtattacct gttggcgctg gatgcaggca ccggaagtat
    24841 tcgggctgtg attttcgacc tgaaaggcaa tcaaatagca gtgggacagg cggagtggcg
    24901 gcatctggca gtaccggacg ttcctggttc tatggaattt gatctcaaca aaaactggca
    24961 actggcgtgt gagtgtatgc gccaggcgct gcacaacgcc ggcatagacc cggagtatat
    25021 cgctgccgtt tcggcatgtt ctatgcgtga aggcattgtt ttatataaca atgaaggaac
    25081 accgatctgg gcctgcgcca atgtggatgc cagagcagcg cgcgaagtta gcgaacttaa
    25141 agaactgcac aacaatacct ttgaaaacga agtttatcgc gcgaccggac aaacactggc
    25201 tttaagtgcc atccccagat tactttggct ggcgcaccat cgttccgata tttaccgtca
    25261 ggcatcaacc atcaccatga tcagcgactg gctggcctat atgctcagcg gcgaactggc
    25321 ggtggatccc tctaacgctg gcaccacggg actttgatct aaccacccgt gactggaaac
    25381 ctgcattgct ggatatggct ggcctgcgtg ccgatattct ttctcctgtc aaagaaaccg
    25441 gcacattgct gggcgtggta agttcacaag cggcggaact ctgcggtctg aaggcgggca
    25501 ctccggtggt cgttggagga ggcgacgtgc agcttggttg ccttgggtta ggcgttgtgc
    25561 gtccggcaca aaccgcggtt cttggcggca cattctggca gcaagttgta aatttagccg
    25621 cgccggtgac agacccagaa atgaacgtgc gcgttaatcc tcatgttatt cctggcatgg
    25681 tacaagctga atctataagc ttttttaccg gactcaccat gcgctggttc cgcgatgctt
    25741 tctgtgccga agaaaaactg attgccgaac gtttaggcat cgacacctat acgctgctgg
    25801 aagagatggc cagtcgggtg ccgcctgggt cgtggggcgt aatgccgatc ttctccgaca
    25861 gaatgcgctt taaaacctgg tatcacgctg cgccttcctt tattaacttg tccattgacc
    25921 cggataaatg taacaaagcg acattgttcc gtgcgctgga agaaaatgcg gcgattgtat
    25981 cagcgtgtaa cttgcagcaa attgctgatt tctcgaatat tcatcccacc tcgctggttt
    26041 ttgctggcgg aggttcaaaa gggaaattat ggagtcaaat tctcgctgat gtctcgggat
    26101 tacccgtcaa tattccggta gtcaaagaag ccaccgcatt aggatgtgcc attgcagctg
    26161 gcgtcggtgc cggaattttt tcatcaatgg cagaaaccgg agaacgcctg gttcgctggg
    26221 aacggacgca cacaccagac ccggaaaagc atgaacttta tcaggattca cgcgataagt
    26281 ggcaggcagt ttatcaggat cagctggggc tggttgatca tggactgacg acgtcgttat
    26341 ggaaagcgcc tgggttatag tttcattgac ggccacatca tgatgaccgt ggctgttagt
    26401 ttttgagtat agtttttctt atttcccttc ttttagagct ggcttgttaa atgccagctt
    26461 gcagattcat cgttccatca ccagtactat attcaaggga aaattgctat ttttcttaaa
    26521 gcattaagca taacccttat tttatagcga atattcctaa agacattaat ggatgcaaaa
    26581 ggggtttaat
//
LOCUS       DAZFWL020000056        26560 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFWL020000056 DAZFWL020000000
VERSION     DAZFWL020000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.056"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1074
                     /locus_tag="ACHKNP_RS12450"
                     /old_locus_tag="pgaptmp_002490"
     CDS             <1..1074
                     /locus_tag="ACHKNP_RS12450"
                     /old_locus_tag="pgaptmp_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HGU4704638.1"
                     /translation="MRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDY
                     VVKGIIPAVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGI
                     GVPRRVKAWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIIL
                     DTLARCFGGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRA
                     SLDAEYRIRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVV
                     DDPRPPVELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNF
                     RRWLYKLEKDGVIRIDGDDVAPL"
     gene            1275..1526
                     /locus_tag="ACHKNP_RS12455"
                     /old_locus_tag="pgaptmp_002491"
     CDS             1275..1526
                     /locus_tag="ACHKNP_RS12455"
                     /old_locus_tag="pgaptmp_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704639.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            1523..1828
                     /locus_tag="ACHKNP_RS12460"
                     /old_locus_tag="pgaptmp_002492"
     CDS             1523..1828
                     /locus_tag="ACHKNP_RS12460"
                     /old_locus_tag="pgaptmp_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704640.1"
                     /translation="MSEPKFGEKLYKHNGLITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            1838..2248
                     /locus_tag="ACHKNP_RS12465"
                     /old_locus_tag="pgaptmp_002493"
     CDS             1838..2248
                     /locus_tag="ACHKNP_RS12465"
                     /old_locus_tag="pgaptmp_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4704641.1"
                     /translation="MTAQIAAYGRLVDDPLVKQSSKGTPMTLARMAVSLPCSQAHDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGDDDPYGDDIPF"
     gene            2259..2531
                     /locus_tag="ACHKNP_RS12470"
                     /old_locus_tag="pgaptmp_002494"
     CDS             2259..2531
                     /locus_tag="ACHKNP_RS12470"
                     /old_locus_tag="pgaptmp_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4704642.1"
                     /translation="MVHDRIAEELEAKGFYRRASARWGEVMLLVETDKERHQVTMRRL
                     ECSRKAQRPPEPPDNYGDLKKAVDRTYAEMGMDGAGDEIWRNYQDS"
     gene            2488..2616
                     /locus_tag="ACHKNP_RS12475"
                     /old_locus_tag="pgaptmp_002495"
     CDS             2488..2616
                     /locus_tag="ACHKNP_RS12475"
                     /old_locus_tag="pgaptmp_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGU4704643.1"
                     /translation="MVLVMKYGEITRTANHTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            2657..2881
                     /locus_tag="ACHKNP_RS12480"
                     /old_locus_tag="pgaptmp_002496"
     CDS             2657..2881
                     /locus_tag="ACHKNP_RS12480"
                     /old_locus_tag="pgaptmp_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704644.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKGIRD"
     gene            3173..4330
                     /locus_tag="ACHKNP_RS12485"
                     /old_locus_tag="pgaptmp_002497"
     CDS             3173..4330
                     /locus_tag="ACHKNP_RS12485"
                     /old_locus_tag="pgaptmp_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4704645.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGATFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            4370..4942
                     /locus_tag="ACHKNP_RS12490"
                     /old_locus_tag="pgaptmp_002498"
     CDS             4370..4942
                     /locus_tag="ACHKNP_RS12490"
                     /old_locus_tag="pgaptmp_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGU4704646.1"
                     /translation="MGEKIMKSMEIRSSEITTSGSNTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            4944..6155
                     /locus_tag="ACHKNP_RS12495"
                     /old_locus_tag="pgaptmp_002499"
     CDS             4944..6155
                     /locus_tag="ACHKNP_RS12495"
                     /old_locus_tag="pgaptmp_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4704647.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPAALHPYPPQSVNMEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            6152..6490
                     /locus_tag="ACHKNP_RS12500"
                     /old_locus_tag="pgaptmp_002500"
     CDS             6152..6490
                     /locus_tag="ACHKNP_RS12500"
                     /old_locus_tag="pgaptmp_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU4704648.1"
                     /translation="MNIGRLRDRVTIQTLKQTRAMTGEILETWLDGHTLWASVNMISS
                     KEAISSGAELATGTVRIWIRYRKDINATSRIKVNTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            6487..6780
                     /locus_tag="ACHKNP_RS12505"
                     /old_locus_tag="pgaptmp_002501"
     CDS             6487..6780
                     /locus_tag="ACHKNP_RS12505"
                     /old_locus_tag="pgaptmp_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4704649.1"
                     /translation="MTELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRRF
                     DDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            6780..7220
                     /locus_tag="ACHKNP_RS12510"
                     /old_locus_tag="pgaptmp_002502"
     CDS             6780..7220
                     /locus_tag="ACHKNP_RS12510"
                     /old_locus_tag="pgaptmp_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGU4704650.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPIDGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREDEERAANRNNWMYDADE"
     gene            7204..7386
                     /locus_tag="ACHKNP_RS12515"
                     /old_locus_tag="pgaptmp_002503"
     CDS             7204..7386
                     /locus_tag="ACHKNP_RS12515"
                     /old_locus_tag="pgaptmp_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704651.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            7509..7865
                     /locus_tag="ACHKNP_RS12520"
                     /old_locus_tag="pgaptmp_002504"
     CDS             7509..7865
                     /locus_tag="ACHKNP_RS12520"
                     /old_locus_tag="pgaptmp_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HGU4704652.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            7849..9510
                     /locus_tag="ACHKNP_RS12525"
                     /old_locus_tag="pgaptmp_002505"
     CDS             7849..9510
                     /locus_tag="ACHKNP_RS12525"
                     /old_locus_tag="pgaptmp_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4704653.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLVSRHYLPEFQLQNPANKNRA
                     IYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            9524..9805
                     /locus_tag="ACHKNP_RS12530"
                     /old_locus_tag="pgaptmp_002506"
     CDS             9524..9805
                     /locus_tag="ACHKNP_RS12530"
                     /old_locus_tag="pgaptmp_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704654.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRALELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(10564..10707)
                     /locus_tag="ACHKNP_RS12535"
                     /old_locus_tag="pgaptmp_002507"
                     /pseudo
     CDS             complement(10564..10707)
                     /locus_tag="ACHKNP_RS12535"
                     /old_locus_tag="pgaptmp_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
     gene            11119..11484
                     /gene="mdtJ"
                     /locus_tag="ACHKNP_RS12540"
                     /old_locus_tag="pgaptmp_002508"
     CDS             11119..11484
                     /gene="mdtJ"
                     /locus_tag="ACHKNP_RS12540"
                     /old_locus_tag="pgaptmp_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HGU4704655.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            11471..11800
                     /gene="mdtI"
                     /locus_tag="ACHKNP_RS12545"
                     /old_locus_tag="pgaptmp_002509"
     CDS             11471..11800
                     /gene="mdtI"
                     /locus_tag="ACHKNP_RS12545"
                     /old_locus_tag="pgaptmp_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HGU4704656.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(11839..12660)
                     /gene="ydgD"
                     /locus_tag="ACHKNP_RS12550"
                     /old_locus_tag="pgaptmp_002510"
     CDS             complement(11839..12660)
                     /gene="ydgD"
                     /locus_tag="ACHKNP_RS12550"
                     /old_locus_tag="pgaptmp_002510"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HGU4704657.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(12760..12843)
                     /locus_tag="ACHKNP_RS12555"
                     /old_locus_tag="pgaptmp_002511"
     CDS             complement(12760..12843)
                     /locus_tag="ACHKNP_RS12555"
                     /old_locus_tag="pgaptmp_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704658.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(12936..13244)
                     /gene="asr"
                     /locus_tag="ACHKNP_RS12560"
                     /old_locus_tag="pgaptmp_002512"
     CDS             complement(12936..13244)
                     /gene="asr"
                     /locus_tag="ACHKNP_RS12560"
                     /old_locus_tag="pgaptmp_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HGU4704659.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            13515..13619
                     /locus_tag="ACHKNP_RS12565"
                     /old_locus_tag="pgaptmp_002513"
     CDS             13515..13619
                     /locus_tag="ACHKNP_RS12565"
                     /old_locus_tag="pgaptmp_002513"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HGU4704660.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(13668..14921)
                     /gene="ynfM"
                     /locus_tag="ACHKNP_RS12570"
                     /old_locus_tag="pgaptmp_002514"
     CDS             complement(13668..14921)
                     /gene="ynfM"
                     /locus_tag="ACHKNP_RS12570"
                     /old_locus_tag="pgaptmp_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4704661.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            15028..15921
                     /gene="ynfL"
                     /locus_tag="ACHKNP_RS12575"
                     /old_locus_tag="pgaptmp_002515"
     CDS             15028..15921
                     /gene="ynfL"
                     /locus_tag="ACHKNP_RS12575"
                     /old_locus_tag="pgaptmp_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4704662.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRYTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            16056..17276
                     /gene="mlc"
                     /locus_tag="ACHKNP_RS12580"
                     /old_locus_tag="pgaptmp_002516"
                     /gene_synonym="dgsA"
     CDS             16056..17276
                     /gene="mlc"
                     /locus_tag="ACHKNP_RS12580"
                     /old_locus_tag="pgaptmp_002516"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HGU4704663.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            17401..18096
                     /gene="bioD"
                     /locus_tag="ACHKNP_RS12585"
                     /old_locus_tag="pgaptmp_002517"
     CDS             17401..18096
                     /gene="bioD"
                     /locus_tag="ACHKNP_RS12585"
                     /old_locus_tag="pgaptmp_002517"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU4704664.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(18049..19341)
                     /gene="clcB"
                     /locus_tag="ACHKNP_RS12590"
                     /old_locus_tag="pgaptmp_002518"
     CDS             complement(18049..19341)
                     /gene="clcB"
                     /locus_tag="ACHKNP_RS12590"
                     /old_locus_tag="pgaptmp_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HGU4704665.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(19500..20114)
                     /gene="dmsD"
                     /locus_tag="ACHKNP_RS12595"
                     /old_locus_tag="pgaptmp_002519"
     CDS             complement(19500..20114)
                     /gene="dmsD"
                     /locus_tag="ACHKNP_RS12595"
                     /old_locus_tag="pgaptmp_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HGU4704666.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(20157..21011)
                     /gene="ynfH"
                     /locus_tag="ACHKNP_RS12600"
                     /old_locus_tag="pgaptmp_002520"
     CDS             complement(20157..21011)
                     /gene="ynfH"
                     /locus_tag="ACHKNP_RS12600"
                     /old_locus_tag="pgaptmp_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HGU4704667.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(21013..21630)
                     /gene="ynfG"
                     /locus_tag="ACHKNP_RS12605"
                     /old_locus_tag="pgaptmp_002521"
     CDS             complement(21013..21630)
                     /gene="ynfG"
                     /locus_tag="ACHKNP_RS12605"
                     /old_locus_tag="pgaptmp_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HGU4704668.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(21641..24064)
                     /gene="ynfF"
                     /locus_tag="ACHKNP_RS12610"
                     /old_locus_tag="pgaptmp_002522"
                     /pseudo
     CDS             complement(21641..24064)
                     /gene="ynfF"
                     /locus_tag="ACHKNP_RS12610"
                     /old_locus_tag="pgaptmp_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
     gene            complement(24125..26550)
                     /gene="ynfE"
                     /locus_tag="ACHKNP_RS12615"
                     /old_locus_tag="pgaptmp_002523"
                     /pseudo
     CDS             complement(24125..26550)
                     /gene="ynfE"
                     /locus_tag="ACHKNP_RS12615"
                     /old_locus_tag="pgaptmp_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
BASE COUNT         6791 a         6512 c         7108 g         6149 t
ORIGIN      
        1 atgcgggaga actggcgcga atacctggaa cgggtgcgtg ggaaagagcc ggcggaagaa
       61 aaaaaccacc agcgaaaaac cacgctaccg atgagcgttg gatctaccgg atacgacacg
      121 caactggatt acgtggttaa ggggattatt ccggcggtat cgctatgcag catatacggg
      181 gctagcgggt cctataaatc attccttgcc ggatcgtggg cgtgccatgt tgccactggt
      241 cgccagtggg gaggccgcag ggttgcgcat ggtgcggttc tctatgtggt tggtgaaggc
      301 ggtataggtg ttccgcgtcg tgtaaaagcc tgggaggttg tgcacgatga gcaggtgaaa
      361 aatctgtatc tggtaaaccg ccccatcttt ccggctgccc cgcttgatgt tgatgaaatg
      421 gttatcgctg cccgtcaggt ggagcgggaa acgggtaaac ctgtacgcat gattattctg
      481 gatacgctgg cgcgttgctt tggtgggaat gatgaaaatg attcccgtga tatgggggcg
      541 tttatccgtg gttgtgacga actgaaacga cgcacagggg ccacggtgct ggtggttcac
      601 cattccggca aggatgagac gaaaggcgcg cgcggttcca gtgcatttcg tgcttcgctg
      661 gatgctgaat accggatacg cagggaggac gcaggaagcg aagcgctggt tatctcatgc
      721 accaaaatga aggacgcgga ggaactcaaa gaagccgcat atgacttacg cgtggtggag
      781 ctttttaccg acgctgacgg tgaattaatc acgtcgctgg tggtggtgga tgatccgcgc
      841 cctcctgttg aactggagcg catcgaggag gcagggaaca agacggaaaa ccataccgcg
      901 ctatgggggt gcatccgttc acgcacacag aacggcgaca agtgcacgat cccgctgtta
      961 cgtgatgaca tgaaaaagct ggggtatgaa atgaaaaact tccggcgctg gctgtacaag
     1021 ctggaaaaag atggggttat tcgtatcgat ggggatgatg tagcgccgct ataaaagtga
     1081 ggagcaaaag cgaggtggat agaaagaggg ccaaaattag cctgctctcc ctcacttttc
     1141 gacctgtata catcctcaaa agtgaggggt aaaaaaatac ttatgaaaca cacacataga
     1201 aaaaccgaaa atcccagctg cgacgaagtg agacgcttga aaaagtgagg cgaaaaagtg
     1261 agaggttgcg agaaatgacc caaaaacgca gagacagaac agagccaaaa tataaagcgt
     1321 tagacatgac tgagcacacc ttaaaggtgg caatcagaac gatagaccgc cacacgcggg
     1381 aaggatacgc gaaggaacat cccgacctga taagcgcatt catgaccacg gcggcggcaa
     1441 actttgccac gctgacagaa cgggagattg ccgaagcgga acaggtaaca accatcaacg
     1501 ttaaaaccgg agaggtggaa tcatgagcga accaaaattt ggtgaaaaac tttacaagca
     1561 caacggactg atcacgattc tgcaaataag cgcagctaca ccaggctggt gggttgaaac
     1621 agacgaagga tcctcacccg tggcatcatg ggcgctttgt gtgcttagtt accctgaccg
     1681 cgatgtgtat caggatattc tgcctgtgat atccactgac aaaggcatga aacccgtaga
     1741 catcaaaaac atgggttttc agtgcgtaat gctcactgaa aaaatgatgg aagagatgaa
     1801 aaagaacaac gccggatcgc ttcactgagg tggcaatatg acagcacaaa ttgcagctta
     1861 cgggcgcctg gtggacgacc cgctggtaaa acagagcagc aagggcacac cgatgactct
     1921 ggcgcgtatg gcggtatcgt tgccatgcag ccaggcacat gacggacagg cgacgttatg
     1981 gttatcggtt atcgcatttg gcaaacaggc cgatttcctg gctaaacatc aaaaaggcga
     2041 tgttgccagc gtatccggca cgatgcaggt aagccagtgg accggacaga acggggaaac
     2101 acggcagggg tatcaggtta ttgcagacag cgtaatcagt gcccgtgcgg cacgtcctgg
     2161 cgggaacaga cgcaaaacca caggcacaca gggtaatcag ccaccagcgg gagacgatga
     2221 cccctacggt gatgatattc cgttctgagg gggtgacgat ggtacatgac cgcatagcgg
     2281 aggaactcga ggcaaaaggc ttttaccgga gggcgtcggc gcgatggggt gaagtcatgc
     2341 tgctggtgga gacagacaag gaacggcatc aggttacgat gcgacggctg gaatgttcca
     2401 ggaaggcaca gaggccaccg gagccgccgg ataattacgg agacctgaaa aaggcggtcg
     2461 atcgcactta tgccgaaatg ggtatggatg gtgctggtga tgaaatatgg cgaaattacc
     2521 aggacagcta atcacacagc cggagaaatc cggctttttt tgcaccagtt gaaacggtat
     2581 ggcgcattac cgggttttcg tcacggtcag gcatagttac tatctgaaac aaacagacac
     2641 aacagaggaa aaaacaatgc cgatgaaatt tgatgagata ttaaaacagc gtgataaata
     2701 ccatgctgac aacatggaga cgatgagcat caatgattac cgcgcattcc tggagacggg
     2761 cgcactgatt gaaaaggatc agcatggttt tgtgagatgt gctctgtccg gtgaaatgct
     2821 ggcggtaaat cctgaacaga tagatgcatt gatagaattt ctgaaaggga tcagggactg
     2881 agcacgcata cagccggagc aatccggctt tttgtcattt tttgtaaatt atttgttcgc
     2941 ggttgttcca cgttgttcac tgacaggatc ggcatatttt acccgaactg aatcatgatt
     3001 attctcgccc gtggtgccag gacgctgggg ccacttttcc gcctgttaat gttctcgcca
     3061 atattcatta ccaggcggga aaacgatcgg tgcgattgct gatttcctta tgaaaaacgg
     3121 ttgagttttt gccgcgtcct ggagttcctt acttaacccc aggacttttt ttatgccgag
     3181 aataatcgaa ttacgccagc agaaaaccgc cattaaaaat cagatgcgcg acatgctgga
     3241 gaacgcggaa aaagaaaacc gcagtcttaa cgatgctgag ggcgcaacat ttgacgaatt
     3301 acgcgctaaa gctgaatccc tcgataaaga catttcccgc cttgaagcca ttgcagacga
     3361 agagcgcagc aagccaggta aaagcagtca gaccactgac cccgcagaac tccgcaacta
     3421 cattctgaca ggtgaaaccc gcgcattaag tacaggcgtt cccgctgatg gtggttatac
     3481 cgttatccct gaactgaaca ccgaaatcat gcgaatgctg gcggatgaat ccaccatgcg
     3541 ccgcatctgt accgtgaaga aaatcagcag caacgagttt aagcagcttg tttccgctgg
     3601 cggtgcgacc gttaaccacg gtgaagaggg taaggcacgc gaacagacca gcaccccgca
     3661 gattaacgag gtgagcatta agctgtatcc ggtctatgcg tacccgcgca ccacacagga
     3721 aatcgtggat ttttccgatg tggacatcct ttcatggctg acgggtgaga ttggcgacac
     3781 cttcacggaa accgaagaaa gcgatctggt tgtgggcgat ggtgacaaaa aagcaaaagg
     3841 ctttttatcc gtaccccgtg cagagaagaa cgacaaagag cgtgattttg gtacgttaca
     3901 ggtaattaaa ccttccgaat ctctggcgtg gacatctgcg gacccgctga tcgacctgaa
     3961 atttgcatta cgtaaaaaat accgcaaaaa cgcggtctgg gtggttaact ccacgacggc
     4021 ggcaaaactc cagaaggtga agaacgcgaa cggtgattat atctggcgcg accgtttaca
     4081 ggcgggtgat cctgatacgt tgctgggcct tccggtcgaa tatctggagt ttatgcctga
     4141 taacgttatt gccctgggtg acttcaaacg cggttactac attgttgatc acgaaacagg
     4201 tgttcgcacc agaccggaca acctcacaga gccgggcttc atcaaaattt tcacgcagaa
     4261 atatttaggc ggtggcgtgg tggattcgaa cgcgatcaag attctggaac tgccacagga
     4321 cgacgattaa cagcatacag aaggggctta aaagcccctt tagtgtttta tgggtgaaaa
     4381 aattatgaag agtatggaaa tccggtcatc ggaaatcacc accagcggca gcaacacgct
     4441 tacaggctac gttgttcgct gggataagct ttcagaactg ttatggggtg agttttacga
     4501 aaaattccag cggggggcgt ttactgaatg gcttgcggcg ggtaatgacg ttcgcggcct
     4561 gtatgagcat gaccacagta tgttactggg gcgcacccgt tccggcacgc tgaaactgga
     4621 agaggatgaa acagggttac gctttgaact gaccccaccg gataccagta cagggcgcga
     4681 cgttatcgaa ctggttaagc gtggtgatat atccggcatg agctttggct ttcgttcccg
     4741 taaggatgta tgggatacca caacagatcc atgcgtgcgc accgtgctgg ttgcggaact
     4801 gtacgaaatt accgtcacat ccgtaccggc ttaccctgat tccggcgtgg agctggcccg
     4861 ccgctccctg tacgagcagc accccgaaaa aatgccgcgt gcggataatc gccgctggtg
     4921 ggcggattta gcgggggtgt gatatgtggc ctttcagaag aaaaaaagag cagcgcagca
     4981 tgacgcttga tgagtttatg gcgctggctg gcacatcgaa cacgggggcg ggtgagtacg
     5041 tatcatcggg gacagcggaa tcactgcccg ccgtcatgaa cgccgtgacg gtcatctctg
     5101 aggcggtggc taccatgccg tgttacctgt acctggtacg caatgagaag gggaaggagg
     5161 cccgcgagtg gcttgattct catccggtcg atcacatcct caacgagcgc ccgaacgcgt
     5221 ggcagactcc ctaccagttt aagcgaatga tgatccgcca ctgcctgtta aacggtaatg
     5281 cttacgcggt gattcagtgg gggcgtgatg gttttccggc ggctttgcat ccttacccgc
     5341 cgcagtcggt gaacatggag cagacaggag aacacaactg gcgctactgc atcactgacg
     5401 cttacaccgg aaacacccgc aactatttac cgtgggaggt tctccacctt cgttactcca
     5461 cggatgacgg ttttatgggg cgctcacctg taaccatctg ccgcgaatcg ctggggcttg
     5521 ggctggccca acaacgccac ggcgcgagcg tgatgcgtga tggcatgatg gcggcagggg
     5581 ttatcacgtc aggcgaatgg ctggacggcg tgaagggcaa acaggcatta gccgcactgg
     5641 aacgctacaa aggggccaga aacgccggaa aaacgcccat ccttgaaggg ggtatgagct
     5701 atcagcagct gggcatgagt aatcaggatg ctgaatggct ggcctcccgt cgcttcacca
     5761 ttgaagacat cgccagaatg ttcaacgtct cgccgatttt tttgcaggaa tacagcaaca
     5821 gcacctacag caatttcagc gaggcaagcc gcgcatttct caccatgacg atgcgcccgt
     5881 ggctggcgaa ctttgagcag cagataaaaa acgccctgct ggtggcctcg cctgtacctg
     5941 gtatccggta tcaggtggag tttgacagcg cggacctgtt acgggccaca cctggcgaac
     6001 gctttgccac ctatgagcgc ggcatcaaat ccggcgttat gtgtccgaac gaagcccgcg
     6061 aacgtgaagg actgtcaccg cgtgatggtg gtgatgagtt cagccaggca tggaaacagg
     6121 aagtaaaaat cagcgaggga gaaaaaccgg aatgaacata gggcgactgc gtgacagggt
     6181 aacgattcag accctgaaac agaccagagc catgacgggt gaaatactcg aaacgtggct
     6241 ggacggtcac acactctggg caagcgtgaa catgatcagc agcaaggagg ccatttcatc
     6301 aggtgcagag ctggcgactg gaactgtaag gatatggata cgctaccgga aggacatcaa
     6361 cgccaccagt cggataaagg tcaatacggg gccgctggcg gggcgtgtac tgaatatcat
     6421 cgggcaaccg ctgccagatg ccgccaggac acgccttgaa attctttgtc gtgagggcgc
     6481 ggaaaaatga cagaacttat caccctggaa gaagtgaaac tccattgccg catcgatggc
     6541 gacgatgaag accagttaat cagcggatac attgccgcat cgcttgaggc gtgccagata
     6601 cacataggca ggcgctttga tgacgggctg gagttcacgc cagccataaa gattggctgc
     6661 atgatgttta tcgctcacct gtatgaaaat cgccagattg tcgcggataa cgcaaaaacg
     6721 cgcgtaccca tgacgatagg cgcactctgg acggcttacc gtgatgtggg ggtgtactga
     6781 tgccgtggca accattaaga cgatgtacag aaccaggctg taacaggcgc gttaagtccg
     6841 gtaagtgtga ggagcacagg cgggcggcat ggcgtgcaga ggatgccaga cgaggacacc
     6901 gccgcgcgcg tgggtactcc agacagtggg acaaataccg cgccatgtat ctgagtaaaa
     6961 acccgttatg cgtgcgatgc cttgagaagg ggatatatac gcccgccgtg gtggtggatc
     7021 acattatccc catcgatggc ggcgatgatg ttctcttctg gcccgaatgg aaccatcagc
     7081 cattgtgtca ggcgtgtcat aaccagaaaa cgaaatggct tgatccggca acaaaaagca
     7141 agcgtgccgc aggtggattt cgtgaagatg aagaacgggc cgctaaccgc aataactgga
     7201 tgtatgacgc tgatgaatga gcgggaacaa aaccgcctta tccgtggact tataaggcag
     7261 cgtgacgcat ggaagacaca ggaaacaggg cataaagata aagcgtcagg acgcgcagaa
     7321 cgcatcacag cgaagcgatt aaccgaccgt gaccgcgagg ttatggaatg tttccgcaat
     7381 cgctgatgag gctgtatggc ggggtggggg gcgttttcag gacaaacccg accccgccgg
     7441 gcaccgaacg cctccccaaa tttttatgca cgggaatttt ttgaaaaata atctgacgaa
     7501 aaataagcat ggcaagacca ccgaaagccc ccgcctacct ggatgacatc gccgtgaagc
     7561 agtggcggga aaaatcgcgg cagcttgcgg aacggggaga cctgaccccc gccgactgga
     7621 gcaatctgga actgtattgc gtcaattact cgatataccg caaagccgtc gcagaccttg
     7681 cggcgcgcgg gttcagcatt gttaacagtc agggcggcga gagcagaaac cccgcattaa
     7741 gcgcgaaatc cgacgctgaa agagtgatga taaaaatggc ctccttgctt ggttttgacc
     7801 cgataagccg ccgtaaaaat ccaccggaaa cagaagaaga ggacgagctt gaccgcctgg
     7861 aataagtacg cagaagacgt aaaaacgggc aaaattccgg cctgtaaacg gctgaaacag
     7921 gccgttaaac ggtacttttc ggatcttaaa agcccccttt acacgttcga tcgtgaggtt
     7981 gtggagcggt ttattgcctt ttccagggtg tgcccgcacg taaaaggacc gatgcgtggc
     8041 agacccattg agctggagcc gtggcagcag ttcgcctttg cgtgcatcct cggctttaag
     8101 gttaaggcca ccggacggcg caaatacacg agcgccttta tcgaagtgcc gcgcaaaaat
     8161 gcgaaatcaa cgaccgccgc gattctggct aactggtttc tgattatgga gaacggtcag
     8221 caggatattt acaccgcagc ggtgagccgt gaccaggcgc ggatcgtctt tgatgatgcg
     8281 cgtcagatgt gccttttatc ccgaccgtta cgcaggcggg tgaatattca ggcgcataag
     8341 gtgatacacc cgaaaagcaa cagcctgtta aagccgctgg cagcaaaagc ggcaaccatc
     8401 gagggcacaa accccagcct ttccatcgtg gacgaatacc acctacaccc cgataacggc
     8461 gtttattctg cgcttgagct ggggatgggc gcacgtcccg aagggctgtt atttgccatt
     8521 accacatcag gcagtaacgt cgtttcagcc tgtaagcagc actatgatta ttgctgccag
     8581 atcctggacg gcgaagaggt caacgattca atttttgtac tgatttacga actggacgac
     8641 gaaaacgagg ttgatgatcc ggcgatgtgg ataaaagcga accctaatat tgatgtttcc
     8701 gtggatcgtg aaaaactggc ctcaaccatc cagaaagcgc ggggtattcc gtcgcagtgg
     8761 gtggagatga tgacaaagcg atttaatatc tggtgtcagg ggactacccc gtggatgggt
     8821 aacggcgcat gggctgagtg tgccggaacg ttcacggagg aagatttaca cggccaggag
     8881 tgttacgcgg ggctggactt atcatcaacc agcgatattt ccagcgtgtg ctatgccttt
     8941 ccggtcggta aaaccattat gctggtttcc cgtcactatc tgccggaatt tcagctacag
     9001 aaccccgcca ataaaaaccg cgccatctat cgtcagtggg ctaaagcagg ctggatacgc
     9061 acaacgcctg gcgactgcat tgattacgac cggatcagag atgacatcat gcaggacgcg
     9121 gagaaattta atatcaggct ggtgggcttt gatacgtgga acgccacgca tctcagaacg
     9181 caattacagg gggcaggttt tgaggtggag ccgttcccgc aaacctacct cagattcagt
     9241 ccggcggcga aatcgttcga agtttttgtt aaccgcaggg tgattgtgca tcgtggcgat
     9301 ccggtgttgt cctggtcgat gagtaacgtc gtgatgcaga gtgacgcgaa cgccaatatc
     9361 aagccgaaca agaaaaaatc accgaacaag atagacccga gcgtggcggc gctgatggcg
     9421 tttggtacat tccaggcaga gcatgaggat tttgcttttg atataagcga cagccaccgc
     9481 cagaaactgg aagaatttag cggggtgtaa tgaggtcagc agcatgacag aagccgaaat
     9541 aacgggatta atccgccgcg ttgccggaat cagccagcag gctgacgaac aggccacgca
     9601 gccggacagc gtgacagccg aaaactatgc gcgtgttgtt gctgaggtga tgcgccgtga
     9661 tggtatagaa cttaatggcg tggatatgcg caacatacga accagagctc ttgagttgct
     9721 ggcctaccgt cgccgcgtgg agatgtatcg ggagaaggag aaaataacgt accactggaa
     9781 gaagccggag cggttgcggc ggtaactggt tgatattccc gacggaccaa aaatgggtgg
     9841 ctacatccct cgcttctgag gactgttaac aagctgcctg aaagacagtc acaaactacg
     9901 aaattttcgc agtttaatta atgatcagct taatcgctaa cccgctgata ttttcggaaa
     9961 cctcaatttg aggaagtcgg cgcggtaact cgctgaactt taagcaaagt gcaaaattgc
    10021 gcttgctgaa taatcattat gattacgcag atgattaagg aatgaccgaa ggcggaaatt
    10081 cgcctgtggt taatggggga gttgcagatc tgcaactcga ccatgaaact acggaaacta
    10141 cccgtagttt gggtagtaag agtaacaccc agattttggg tcttactcgt gatacccaaa
    10201 tttggggtat cagtggcaac cataacgact ttcgttacgg ttgatgcttt taccccattg
    10261 ggggaaaggt attacgataa tcataacacc taccgaacag gtaaagccca ccagcctgat
    10321 taacgtttaa ccggaaaaaa attccggtag gtggggatcc ccatatcgac attaacgccc
    10381 ctcatgaatt ggtgcgcttc cccctggaaa gatgatccgc ctgtatattt cttgtgtcta
    10441 tttgttccac gttgtttcat acagtgcacc gaacggtgta gtcactggtg tagtcacttt
    10501 gcgatttttg gcactttttg cggtgaaaat tatttaatta aatcaacgtg ttatgtgaaa
    10561 taagttatat ataacagcaa taaataatgc gagaataaac ggcacgatga tctcggcggc
    10621 aaaacggata ccgcagagaa taatgaccag cattcccaac ataatgacga tttttaggcc
    10681 attgagcgtg atgatcggct ttgccatgct tgtccccgtt ttttcttcct ttgctaataa
    10741 taatgtcttt ttcgctccgt catcataacg aaaatcaaac cgctcaggta aagaagtgaa
    10801 aatattttga gttaattctt aagctatgat acaaatcagg cgtgttcaac taccgaggac
    10861 aattatcatc cgcgatgacg agaagcaaca ctgcggataa ttgtaatatt atggacaatt
    10921 tgttcaggac gttattttca acatttaccc atcttcgtac ctcatccact atcttgctcg
    10981 taggtgagca gcactggcgc aacgcgcttt agtcctttct tctgcctaag cttgccctat
    11041 gcgcggggct tgaccacttt actttggttt cgctgaatta agcgaaaatt aaaataattc
    11101 tcttgcagga gaaggacaat gtatatttat tggattttat taggcctggc tattgctaca
    11161 gaaattaccg gtacgctgtc aatgaaatgg gcgagcgtca gtgagggaaa tggcggcttt
    11221 attttaatgc tggtgatgat ttctctgtcg tatatatttc tctctttcgc cgttaaaaag
    11281 atcgccttag gcgtagctta tgcgctgtgg gaaggtatcg gtattttatt tattaccttg
    11341 tttagcgttt tgttattcga cgaaagttta tcgctgatga aaattgccgg gttaaccacc
    11401 ctggtcgccg ggattgtgtt gatcaaatca ggtacccgta aagcgcgtaa acctgaactg
    11461 gaagtgaacc atggcgcagt ttgaatgggt tcacgccgcc tggctggcat tggcaatcgt
    11521 gctggaaatc gttgctaacg tctttttgaa attttctgac ggctttcgtc gcaaaatatt
    11581 tggcttgctc tccctggcgg cggtgctggc tgcctttagt gcgctttctc aggccgtaaa
    11641 agggatcgac ttgtctgtcg cttatgcatt gtggggcggg tttggtattg ccgccacgtt
    11701 agccgcaggt tggatcttgt ttggtcaacg gttaaatcgt aaaggctgga ttggcctggt
    11761 cttgctgttg gctggaatga tcatggtgaa acttgcctga tgaacacgct gcccgcactg
    11821 tcgggcagcg tttgaacatt atttttgcga cagttgatcc agcttgtcgc gaaaaccggt
    11881 aacagaaatg gcccggttat cggcgcgcca gcgatctttc gcggcaggag ccgaactttg
    11941 caccccaatt aattgccagc cgtcatcggt atgcaacatc aaaggcgaac cgctgtcacc
    12001 cggcaaggta tcgcactgat gtgacatcac cgacgtttgc gcccagccag tcacttcaca
    12061 gttttgatga ctgtataacg tatcgagatg atcttcagga tatcccgcct gagtcacttt
    12121 acgaccagcc gattttaatg cggcagtaag cgcggcttta tctccctcaa ataacggcaa
    12181 cggtgtaatg ccagaagggg gattacgcag cacaatcaag ccgaagtccc atggtgcagc
    12241 tgcgggagga acaatccaac catccccatc tgcttttaac cgctttccca gtgtcggatc
    12301 aacgcggcct tctatgtcgt ggatgtcata gcgccaaaga cctttatttg acacaaaacg
    12361 cagcgctacc gccttatccg ctttaccctt aggcggcgtc aataaacagt gtcctgccgt
    12421 taatgccaga ttgggtgcaa tcagcgtcgc cgtacataaa ttgccgctgg ccgtttccag
    12481 ttgcccaacc gcatcccacg gtgattgggt cgtgtcattc actggcacac gatcatcatg
    12541 accaaaaaac agggtgctga cctcatcgtt tgccgatttg gcaacgtctg gtttatctgc
    12601 aaacacaaaa gcagacgtca aactaattgc acccaacact acagcaatgg ttgtacgcat
    12661 atcacactct ggtgggtaat tatgattatt aaaagcaaac cctcataaat actatagacg
    12721 ggacgggatg aaagtgggag taaaatcaga taactacagt caggaaagat aaaaacgggc
    12781 ggcaatcagt gcgcataaaa taaggatgat aagaatgaac tcaaagcgat aacggcccac
    12841 catactaccc tccgctaaag gcggtgctaa acgttacctg cttagcaccg taattttcag
    12901 gcgcgagggg gcgcgccagc attactgttg aaaacttacg ctgcgggttg tgcagcaggt
    12961 tttgccggtt gctgatggct gtgtttcccg gcgtgtttct tcgctgcctg cgctttttgt
    13021 tcaggggctt tctgttcagc tttcgtattt ttatgatgct ttttagccgc ctgcgctttc
    13081 tgggcaggtg ctgctttatg ctgtttttta tgatgtgtcg ttttcgccgg cgctgctttg
    13141 gtggtcgtcg cagtcggagc aggtgtggtc gcagtctctg cagcaaaggc ggcagaagac
    13201 agacccatag cagcggcaac aaccagagct aatacttttt tcattgtcat accctcaatt
    13261 tgttttttca tttaacccca ctgcggggcc gttgaaataa ctatatccct gggaatggca
    13321 gacttccgtg agtggttggt ttcagcgtgt aacgatatgt acaaacgctg aataaattac
    13381 agcgttgata ataacgtatt gtgacttaag ggaaatttag ctaccaataa tagtagtctt
    13441 gatcgggtta actattttac tacttacaaa gcggtagaat aactgcgcat cagtaataaa
    13501 tgacacacag caaaatgaat ccgtttattt gggtacttat aatcctgatg acgctagacg
    13561 cgctgcggga attggctggc gcttcgtcta ttttaggatg gctattaacg ttggtttgag
    13621 caagcaataa gtccggacgt gtagttaccg cagtccgggc ttatttttca ggcgtgcaga
    13681 cgacgatgca aacgcgtccc gaccagcaga gcaatgacca gcatcagagc aataaatgcg
    13741 ccgacgccgt tccagccata gttatgccag aaaacaccac ccagcgtccc ggcaatactc
    13801 gaccccagat agtaactgaa cagatacagc gaggaggcct ggcctttagc gcgttttgcg
    13861 cgggggccaa tccagctgct ggcaactgag tgggctgcga agaatcctgc tgagaagagt
    13921 aacattccgg caaagatcag ccacagcgag ctgaataagg tcatcagtaa accaaacagc
    13981 ataacccccg tcgaaaacaa catcaccgga ccgcgcccgt agcgggtggt catggttccg
    14041 gctttgggtg agctccatgt accggtcaaa taagccagcg ataataagcc aaccacggcc
    14101 tgactgacat gccaggggga gagcatcaac cgatatccga tgtaattaaa cagcgtgacg
    14161 aacgacccca tcagcaaaaa gccttctgcg aacaataacg gtaatccccg gtcacgccag
    14221 tgcagacgaa agttgataaa caacgtttta ggtcgcagcg aagtcgggcg aaaatggcgt
    14281 gattcaggga ggattttcca gaacatcaac gccgaggcta gcgcgaaaca accgattgcc
    14341 gccagagcaa ttcgccagtt gaaaaagtcc gtgaagacac cactaattaa gcgtccgctc
    14401 atgccgccaa ttgagttgcc gctgatatac aaccccatcg aaaaggccac gaaactgggg
    14461 tggatttcct cgctaagata agtcatgcca acagctgcca cgccacttaa cgaaagccca
    14521 atcaaggcgc gcataatcaa aatgccgtgc cagctggtca tcattgtcga aagtaacgta
    14581 caaatggagg ccaacagtag cgccgtgacc atcactggtt tgcgaccaat ggcatcggat
    14641 agggggccag taaacagcaa accaatagcc aacatcgccg tggaaatgga cagtgaaata
    14701 ctactgttcg cgggcgttaa gccaaactcc tgcgaaagca ccggaaggat aggctgcaca
    14761 caatagagaa gtgcaaatgt tgccagtccg gcagagaaca gcgccagggt gacgcgcata
    14821 aattgcggcg taccgcgttt aataaattga tttggctgag aaatgctttg cttgtcagtg
    14881 tcgcttgccg gagcgccatc aacagttgta gtacggctca cttgaaatcc ttgctaaata
    14941 tgcctgtgga tcaggcttat acataggtta ggaaaatcga attgttctgt ctaatatatt
    15001 aataatctca aataagatgt tttaaatatg aatattgaac ttcgtcatct gcgttacttt
    15061 gttgctgttg cggaagagct gcatttcggg cgcgccgctg cccgcctgaa tatttcgcaa
    15121 ccaccgctaa gtcagcagat tcaggcactg gagcaacaaa ttggtgcccg actgctggca
    15181 cgaactaatc gcagtgtatt gctgacggca gcaggaaaac agtttcttgc agatagtcgg
    15241 caaatcctgt ctatggtaga tgacgctgcc gctcgcgctg aaagactgca tcagggggaa
    15301 gcgggggagt tgcgcattgg ttttacttcg tccgctcctt ttattcgggc ggtgtccgat
    15361 acgttatcgc tgtttcgccg tgattatccc gatgtccatt tacaaacccg cgaaatgaac
    15421 actcgtgagc aaatcgctcc gctcattgaa ggaacgctgg atatgggatt gctgcgttac
    15481 acagcgttac cggagacgct tgaacacgca gtcatcgtcc atgaaccgct tatggcgatg
    15541 atcccgcacg atcatcccct ggcaaataac ccgaatgtaa cgctggctga actggcgaaa
    15601 gaacccttcg tcttttttga tccgcacgtc gggacagggc tgtatgacga tattctcggg
    15661 ctgatgcgac gttaccattt gacgcccgtc atcactcagg aggtgggcga ggcaatgacc
    15721 atcatcggtc tggtttccgc cggtctgggt gtttcaattt tgcctgcttc atttaaacgt
    15781 gttcagctca acgaaatgcg ctgggtgccg attgctgaag aggatgcggt ttctgaaatg
    15841 tggttggtct ggccgaaaca tcatgaacaa agtccggctg cgcgtaactt tcgtattcat
    15901 ctgctgaatg ctctcaggtg agggaaattt cagcgaaaaa gcccgaaaaa tgtgctgtta
    15961 atcacatgcc taagtaaaaa tttgacgaca cgtattgaag tgcttcacca tagcctacag
    16021 attatttcgg agcgcgaaaa tatagggagt atgcggtggt tgctgaaaac cagcctgggc
    16081 acattgatca aataaagcag accaacgcgg gcgcggttta tcgcctgatt gatcagcttg
    16141 gtccagtctc gcgtatcgat ctttcccgtc tggcgcagct ggctcctgcc agtatcacta
    16201 aaattgtccg tgagatgctc gaagcacacc tggtgcaaga gctggaaatc aaagaagcgg
    16261 ggaaccgtgg ccgtccggcg gtggggctgg tggttgaaac tgaagcctgg cactatcttt
    16321 ctctgcgcat tagtcgcggg gagattttcc ttgctctgcg cgatctgagc agcaaactgg
    16381 tggtggaaga gtcgcaggaa ctggcgttaa aagatgactc gccattgctg gatcgtatca
    16441 tttcccatat cgatcagttt tttattcgcc accagaaaaa acttgagcgt ctaacttcga
    16501 ttgccataac cttgccggga attattgata cggaaaatgg tattgtacat cgcatgccgt
    16561 tctacgagga tgtaaaagag atgccgctcg gcgaggcgct ggagcagcat accggcgtac
    16621 cggtttatat tcagcatgat atcagcgcat ggacgatggc agaggccttg tttggtgcct
    16681 cacgcggggc gcgcgatgtg attcaggtgg ttatcgatca caacgtgggg gcgggcgtca
    16741 ttaccgatgg tcatctgcta cacgcaggca gcagtagtct cgtggaaata ggccacacac
    16801 aggtcgaccc gtatgggaaa cgctgttatt gcgggaatca cggctgcctc gaaaccatcg
    16861 ccagtgtgga cagtattctt gagctggcac agctgcgtct taatcaatcc atgagctcga
    16921 tgttacatgg acaaccgtta accgtggact cattgtgtca ggcggcattg cgcggcgatc
    16981 tgctggcaaa agacatcatt accggggtgg gcgcgcatgt agggcgcatt cttgccatca
    17041 tggtgaattt atttaaccca caaaaaatac tgattggctc accgttaagt aaagcggcag
    17101 atatcctctt cccggtcatc tcagacagca tccgtcagca ggcccttcct gcgtatagtc
    17161 agcacatcag cgttgagagt actcagtttt ctaaccaggg cacgatggca ggcgctgcac
    17221 tggtaaaaga cgcgatgtat aacggttctt tgttgattcg tctgttgcag ggttaacatt
    17281 ttttaactgt tctaccaaaa tttgcgctat ctcaatttgg gccaggaaag cataacttag
    17341 actttcaagg ttaattattt tcctggttta tatttgtgaa gcataacggt ggagttagtg
    17401 atgctgaagc gtttctttat taccggtaca gacacttctg tagggaaaac ggtggtttcc
    17461 cgcgcattgc tacaagcgtt agcctcccag ggaaaaacgg ttgcgggata taaacccgta
    17521 gcgaagggga gcaaagagac acccgaaggg ctgcgtaata aagatgccct ggtgttgcag
    17581 agtgtttcaa ccatcgaact gccttatgaa gcagttaatc ctatcgcgtt aagcgaagaa
    17641 gaaagtagcg tggcgcacag ttgcccaatc aattacaccc tcatttcaaa cggcctggca
    17701 aacctgaccg ataaagtcga tcatgtcgtg gtagaaggga ctggcggctg gcgcagtctg
    17761 atgaatgatt tgcgtccact ctctgaatgg gtggtgcagg aacagttgcc ggtgttgatg
    17821 gttgtcggta ttcaggaagg ttgcattaac catgcactgc taacagctca ggcgattgcc
    17881 aacgacgggc tgccgctcat tggctgggtg gctaaccgaa tcaacccagg actggcgcat
    17941 tatgcggaaa tcattgatgt gctgggtaaa aaacttccgg caccgctcat tggtgaactg
    18001 ccttatctgc cgcgcgccga acagcgagaa ctggggcaat acatccgctt agctatgctg
    18061 cgcagtgtgc tggcggtaga tagagtcacg gtgtaacgtc cgcgaaatta ccgacgcaat
    18121 gacgcaggca atcaataaac cggggagtag ctgatactcc ccggtcattt cacatatcat
    18181 caacgtcgac ataatcggcg cgtgcgtggt tgccgccaac agtgtcgcca tcccggtcaa
    18241 tccgagtaaa agcgtaattt cttcgccatc agggaaccat aatcccaggc tacgaccata
    18301 caacatgcca atggcaagac cgataaatag cgtcggtgta aagacaccac cgggtgcgcc
    18361 ggaaccgcta ctcgccagca cggcacacag tttacaaagg aagatcccgg caatgatcat
    18421 taacagtggt ggggcggtta aaaaggattg tacggtgcta tagccgttgc cccacactgc
    18481 aggtgtgaac agggaaagca gacccacgat caacccgcct agtgccagtt gccagggcgg
    18541 cgcaagtttg agactcacaa atccacgatg acaggcgttc attaacgtta acaacagtgg
    18601 tccgcacaga cctgccagca cacctgtact gataatcagc gcatagtcac gagcctgaac
    18661 cgtcactgag agttgtacgt tgtagagtaa cgcgtcgcta tgattaatca gattgctaac
    18721 cagcaatgcc acgacggcgg aaataatcac cgggccgaga gaggccaaca tcatagtgcc
    18781 aaacagcact tcggctataa ataaactgcc agcaagcggg gcacgatagg ccgcagccat
    18841 tcccgccgcg gccccacagg cgatccataa tttccactcc tggcgtggcg taaaacgttg
    18901 ggcaaaacag gaggcggcaa gggcagctaa aagaatcatc gcaccttcgc gaccaattgc
    18961 actgccgctg gttactacca gcagagaggc aagcgattta accaggcttg ctgcgtaatc
    19021 gaactgtcca tcggtttgca acgcttccat gtaatcggtc ggcgcatgag ggcgttgttg
    19081 ggtaaatttc tgccagccca tcagcaacaa acccgccgcc agtccgccga gcgccggagt
    19141 tagcaaccgt cgccaggggg aaaggtttgt cgctgcattg accagactgc cggagtcatt
    19201 attgaggaac aaccactcca gtagcagcat cgcatgacga aacccggcaa cggcaaaggc
    19261 cgcgagaata ccgacgactg ttgcgataag cagacggcgg aacatagtgc gcagatccgg
    19321 atagatgtgt agatgatgca tgagcagaaa tgggcaatga aacacgggcg actattttgc
    19381 ggcaaaagag gccagccgca aaagcagtgc gtgctttaag agataaaaaa acgtgacact
    19441 gtaacctatt attgccggat tgcgcgtaat cgtcaccatc cggcaatatt acggtgatcc
    19501 tatcgaaaca gcggtttaac cgcgacagga attaacagtt gtgattgcca ttgcgccagc
    19561 gttaagcgag ccagttcacc cagtgcacgg tagaaagggt gttctgcttt ttcgataaaa
    19621 acatcaagaa aacgtgttga ccacggaaaa aggtgccatg ccagcaattc ttcgcattct
    19681 gtctggcgac cattctccgc caaccacgca gccatcaaca gcagtgaacc aaaatgatct
    19741 tccggttcgt tttgcttcat ttcaaactga atgcctttct cgcgcatcca ctgacgaagt
    19801 gccaatgttg aatcgccaaa cagcacagat tcgcgatcca gccagaccga cccccacggc
    19861 ggagacggca gtgcccacgg gccgacaaac aaacgctgcc aggcctgggc gtgagtctct
    19921 tcacactgtg tctgaaaagc cgtcaccagc ggcgctaatg acgcttctgg tagaggccac
    19981 tgagtttccc agccgtcact ggtgagtacc gcaaccagag gtgcggcttc cgcactctct
    20041 ggagcgtaat aaaacagcgc gcccaacacg cgcgccgcga cagaaaaatt atcttgctgt
    20101 gaaaaatggg tcattcctaa catcctgaaa tgtgcgggtt gccccgcacc aatttgttaa
    20161 cctgcaatag ccattcctac tgtcatgtgc aggccataaa agagcacacg cccgatcatc
    20221 tcaccgccaa gtatgaggat tagccccaga ataagtccgg caacgtgagg ttcacggcga
    20281 cgaatgagcg ggcaaagcca gcatccaaga cctgcgcata acaataccac acgccagacc
    20341 tgtaaggagg cgtaatctgg taccagtgcg ctggcttgtt gcacggatga gtgaatagac
    20401 gccagagaca aaccttgcag cacaatcacc ccggcacaag cgatcagcgc cagaacgcta
    20461 atgatggcaa atggtgtggt attaaatgta acgcgggctg cccgcagaat tgcggcagca
    20521 agtattggac cgctcaacaa taccgtcagg aagaaagcca gcgtagtgta accgttatgc
    20581 caggttggca cagtgtcgat ttgatacacg caggtcatca tccagacgaa aatgacgccg
    20641 agcgccatgc tgacaagtaa ccagagtttc cccaacgctt gcggcatttt accgatgacc
    20701 gccaccagcc accatagtcc gccaacggca aaaaatatgg aaccggcagc aatctcgtta
    20761 ctcagaccag aagctccgat tcgattcagc gagttaaacg cccgcagtgg cgatcccagg
    20821 tgcatgacag aggcaataaa cccgatgccc atcaacagcc agaggaaaaa catgccacgg
    20881 acaatacgct gacggtctgc gtcatttttg gcggcgaacc agccaattcc gctaacaatc
    20941 agcgcgccta ctacgcattg acccaatacg gtaaagataa ccagtggcca ttcatgccat
    21001 ccatttccca ttttacactt cctccggatt agccagataa ccagtggtat cgccggtcgg
    21061 gcggctgttg gcgttaggtt tgataacgat attgggtttt gtgaaatgcg cgcgcggcag
    21121 tggcgcgacg gcagccagag tgccgtgctt ctggcgcagc tcttcaattg gaccgaactc
    21181 aagtgcacgc agcgggcagg attcgacgca tatgggttgt ttcccctcgg cgacgcgcga
    21241 ataacaacca tcgcacttcg tcatgtgccc tttttcagca ttgtactgtg gtgcgccgta
    21301 cggacaagcc atgtggcagt agcggcagcc gatacaaaca tcttcatcaa ccaccacaaa
    21361 gccatcttca cgcttatgca ttgcgccgct ggggcagact tttgtacatg cggggtcatc
    21421 gcaatggtta caggagatgg agagataata agcgaacacg ttctggtgcc agacgccatt
    21481 atcttcctgc cagtcgccgc cagcgtattc ataaatacgg cggaaactga cttccgggcc
    21541 aagatcttta aaatctttgc acgccagttc gcaggtttta cagccagtac agcggctgga
    21601 atcgataaaa aatccatatt gtgtggtcat gggctactcc ttaaaccttt tcgatctgga
    21661 caagattgct gtgcgacggg tttccctttg ccagcggtga agggcggtga gaggtcagaa
    21721 tattgatact gccgccgtga tcgacccggt caccaaacat atccgcttta agccacgcac
    21781 cttgcccgat ggcggtaacg ccaggcagaa tacgcggagt cacttttgcg gcaatcagca
    21841 tttctccatt attgttaaat acccgcacgg tatcgccatg acggataccg cgtgcctgag
    21901 catcaatggg gttgatccac acctcttgtg ggcaggcctg ctgtaacaca tcaatattgc
    21961 cgtagctgga gtgggtacgc gctttgtaat ggaagcccgt taactgcagt ggataggttt
    22021 tccgcagggg atcgtcccag ccatcaaaac ctggggtata cgcaggaagg ggatgaataa
    22081 tttcatcttt tttcaattcc caggtatctg caatcttcgc cagtcgttca gaataaattt
    22141 cgattttccc cgaaggtgtt ttcaacgggt ttgcctgtgg atcttcacgg aatgcgcgga
    22201 aagcgacgta gtgttcttcc gggcattttt tcttaaagat cccggtcgtt ttcatctcct
    22261 cgtagtcggg catctcaggg ttacgttcct tcgttttcgc atggagatat ttgacccatt
    22321 catgctgact gcgaccttca gtaaaggttt gataaacgtc tggtcctaag cgtttggcga
    22381 cttcactcag catccagtag atgggtttgc gttcaaattt tgctgaggtt gcgggttggg
    22441 cgaggatcac atagcccata ttccctgcag attcatgaga gataaggtct tcttgctctg
    22501 ttggcatcag gtcgggcaac aggatatcgc aatacttagc cgaggccgtc atgaagtggt
    22561 caatgccaac aatcatctcg cacttgctgt catcctgaag cacctcatgg gtgtgattga
    22621 tgtcgccatg ttgattgatc aatgtgttac tggcgtagca ccataaaaac ttgatgggga
    22681 catccagttt ttcttttcca cgaacaccat cacgggtcgc ggtcatttcc gtaccatggt
    22741 cgatggcatc tgtccatgta aagacggaaa tctgcgtttt aacaggattc tcaagcatcg
    22801 ggaaccattc tacccccaga tcccagctac cttcgcgtac acctgagttg ccgccgttta
    22861 tgccgacgtt accggtgaga acggaaagca tggcaatagc gcgggacgtt tgctcgccgt
    22921 tggaatgtcg ttgtggcccc caaccctgac aaatataagc aggttttgct gaaccgatct
    22981 ctcgtgccaa ctggataatt ttttctgccg ggatgctggt gatttttgct gcccattccg
    23041 gcgttttagc tatgccgtca ggcccttcgc ccagaatata ggctttataa tgcgcgttac
    23101 gtggtgcgtt ggcgggcagc gttttttcat cgtaaccaac acaatatttg tcgagaaatg
    23161 gctgatcgac catgttttca gtaatcagta cccaggcaat cgcacaggcc agtgcgccat
    23221 cggtgccagg gcgaatgggc agccattcat cttcacgccc ggcagcagtg tcgttataac
    23281 gtggatcgat gacgatcatg cgtgcgtttg aacgttcgcg ggcttgctcg acgtagtaag
    23341 tgacaccacc gccgctcatc cgcgtttctg ccgggttatt tccgaacata acgaccagtt
    23401 tcgtattggc gatatcatcc gggctgttgc catcattggc accgaacata taactcattg
    23461 cggcactgat ctgtgcggta ctgtagctgc catagcgact gagaaaacca ccgcaagagt
    23521 tcatcagacg gtacgggacg tttgagttgg tgatgtttcc gccatctacg cctgttccgt
    23581 acaggacatg tacagcctca ttgccgtaat ctttcaggat ccgccgaaga ttatcactga
    23641 tggtatccag ggcttcgtcc caacttatcc gttcaaatta accttcaccg cgcttgccga
    23701 cgcgcttcat gggatatttc aacctatcag gatgattcat ccgtcggcgg atagagcgcc
    23761 cgcgtaaaca cgctcgaacc tgatggttac cgtagacgtc gtcacctgtc gtatcagact
    23821 ccacccagta cacggtgtca tctttcacat gcaaacgtaa cagacagcgg ctcccgcagt
    23881 taacggtgca ggaactccag accgctttct cttctaccgg agcctctgcc gcccggacca
    23941 tttgggaaaa tggcagagtg aaagcactgc ttgccagcgc aagactgcca agtgcggagg
    24001 ttttcatcag acttctacgg ctgatttcag ccttcatgag cgcctctgtg gtatggattt
    24061 tcatcattac tcacttattg cttttcaaac aaaatgtcat gccagaattt atggttgtcg
    24121 tgggttatat tttttcgatc tcgaccagat tagtgtgctg cgggtttccc ttcgccagtg
    24181 gtgaagggcg cagagtggtt agcgtattca cacagccgcc atggtcgatt ttatcgccag
    24241 acatattggc ctcgtgccag gctccctggc ccatagcgct aactccaggg agaatacgtg
    24301 gtgttacttt ggctggtagc cgaacttcgc cacgatggtt aaacacccgc accatatcgc
    24361 cgttggcaat cccacgtttc tgcgcatcta tagggttgat ccacacctcc tgacggcagg
    24421 cagccttcag gagatcaata ttgccgtagg tcgagtgagt acgggatttg taatggaaac
    24481 caaacagttg cagtgggaag gttctacgtt caggggagtt ccagccttca aaggttgagg
    24541 cataaactgg caatgggctt atcacttcat ctttttccag ttcccaggta cgggcaattt
    24601 ccgccagcct gctggaataa atttcaatct taccggaagg cgttttaagt ggatttgcct
    24661 cggggtcgtc acgaaatgct ttgtaggcga caaaatggcc attgggatct ttacgcttat
    24721 agatacccat tttttcagtt cgtcgtaaga cggtaacgcc ggatctttgg caagcatttt
    24781 ggcgtacaga tgttgtaacc attgttcctg cgtgcgacct tctgtgaact tttgatagac
    24841 gtcaggtcca agacgtttcg cgacttcact caggatccag taaatcggtt tgcgttcgaa
    24901 tttttcgctg gtgacaggct ggaggaaaat gagatatccc atgttaccgg cgtagtcgtt
    24961 aggaataata tcttcctgct caacggtcat caggtctggc agcagaatgt cggcatattt
    25021 tgccgatgag gtcataaagt tttcgatgat cacaatcatt tcgcatttcg attcgtcctg
    25081 cagaatttca tgcgttttgt tgatgtcaga atgctgatta acgagggtat ttcccgcgta
    25141 gttccagatg aacttaatgg gcacatccag tttatctttg ccgcggacgc cgtcgcggat
    25201 tgccgtcatt tgcggaccat gatcgatagc atctgtccag ctgaagcagg agattgacgt
    25261 tttgaccgga ttatccagca ccggcaggcg ttctatggta atggtatagg tcgattcacg
    25321 cgcgccacta tttccgccgc tgatgccgac attgcccgtc aaaataggta acatagcaat
    25381 agcgcgtgca gtcagttcgc cgtttgcctg gcgttgcggc ccccagccct ggcagatata
    25441 agcgggtttt gctgtgccaa tttcacgcgc cagtttgatg atacggtcta ccgggatacc
    25501 ggtaatttgc gaagcccact gcggcgtttt cgctgtgtta tcatcacctt caccaagaat
    25561 ataggcttta tagtgaccat ttttgggtgc atctgcgggt aaggtttttt cgtcatagcc
    25621 gacgcagtat ttatcgagaa aaggttgatc aacgagattt tcgttaatca atacccaggc
    25681 aatacccgca accagcgcgg catcggtgcc cgggcgaata gggagccatt catcttcacg
    25741 accagcagcc gtatcggtat atcgcggatc gataacaatc attttggcgt tcgatttctc
    25801 gcgcgctttt tcaagaagat aagtgatgcc accgccgctc atgcgggttt ctgccgggtt
    25861 gttaccaaac atcacgacca gcttgctgtt ttcaatatcc gtggtgctgt tgccatcatt
    25921 actgccgtag gtgtagggca tggcacagga aatttgcgcg gtgctgtagg agccatactg
    25981 attgagtgaa ccgccgtagc agttcatcag gcgtttgacc gccgaggctg atggcgaaga
    26041 gcgggtcata ttgccgccaa caatccccga agagtactga atatatacag cctcattgcc
    26101 atattgttcg acggtttttt tcaggctact ggcgatagta tccagggctt catcccagct
    26161 aatccgttcg aatttgcctt caccgcgtgt acccacgcgt ttcattgggt aattcaagcg
    26221 atcgggatga ttaatacgcc ggcggatgga gcgaccgcgc aaacaggcgc gtacctgatg
    26281 gttgccgtac tcatcgctgc cggtattgtc agtttccacc caggtcactt cattatcttt
    26341 aacatgtaga cgaagtgcac agcggctacc acagttgacg gaacaggcac cccagatcac
    26401 tttttcgctg gcctgttgta ccgttgccgc tgcactgcgc agggtgaacg gtaaagaaaa
    26461 accgcctgca gccagcgcca gagaacctat cgcggttgat ttaacgagtg ttctgcggct
    26521 gatgcccacc attcggtcat ttttggacat aactcactcc
//
LOCUS       DAZFWL020000057        26503 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFWL020000057 DAZFWL020000000
VERSION     DAZFWL020000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.057"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHKNP_RS12620"
                     /old_locus_tag="pgaptmp_002524"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHKNP_RS12620"
                     /old_locus_tag="pgaptmp_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGU4704669.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            414..608
                     /gene="bfd"
                     /locus_tag="ACHKNP_RS12625"
                     /old_locus_tag="pgaptmp_002525"
     CDS             414..608
                     /gene="bfd"
                     /locus_tag="ACHKNP_RS12625"
                     /old_locus_tag="pgaptmp_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HGU4704670.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            681..1157
                     /gene="bfr"
                     /locus_tag="ACHKNP_RS12630"
                     /old_locus_tag="pgaptmp_002526"
     CDS             681..1157
                     /gene="bfr"
                     /locus_tag="ACHKNP_RS12630"
                     /old_locus_tag="pgaptmp_002526"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HGU4704671.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1154..1621)
                     /locus_tag="ACHKNP_RS12635"
                     /old_locus_tag="pgaptmp_002527"
     CDS             complement(1154..1621)
                     /locus_tag="ACHKNP_RS12635"
                     /old_locus_tag="pgaptmp_002527"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGU4704672.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHKNP_RS12640"
                     /old_locus_tag="pgaptmp_002528"
     CDS             2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHKNP_RS12640"
                     /old_locus_tag="pgaptmp_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGU4704673.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2344..2973
                     /gene="rplC"
                     /locus_tag="ACHKNP_RS12645"
                     /old_locus_tag="pgaptmp_002529"
     CDS             2344..2973
                     /gene="rplC"
                     /locus_tag="ACHKNP_RS12645"
                     /old_locus_tag="pgaptmp_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGU4704674.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2984..3589
                     /gene="rplD"
                     /locus_tag="ACHKNP_RS12650"
                     /old_locus_tag="pgaptmp_002530"
     CDS             2984..3589
                     /gene="rplD"
                     /locus_tag="ACHKNP_RS12650"
                     /old_locus_tag="pgaptmp_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGU4704675.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3586..3888
                     /gene="rplW"
                     /locus_tag="ACHKNP_RS12655"
                     /old_locus_tag="pgaptmp_002531"
     CDS             3586..3888
                     /gene="rplW"
                     /locus_tag="ACHKNP_RS12655"
                     /old_locus_tag="pgaptmp_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGU4704676.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3906..4727
                     /gene="rplB"
                     /locus_tag="ACHKNP_RS12660"
                     /old_locus_tag="pgaptmp_002532"
     CDS             3906..4727
                     /gene="rplB"
                     /locus_tag="ACHKNP_RS12660"
                     /old_locus_tag="pgaptmp_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGU4704677.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHKNP_RS12665"
                     /old_locus_tag="pgaptmp_002533"
     CDS             4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHKNP_RS12665"
                     /old_locus_tag="pgaptmp_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGU4704678.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5037..5369
                     /gene="rplV"
                     /locus_tag="ACHKNP_RS12670"
                     /old_locus_tag="pgaptmp_002534"
     CDS             5037..5369
                     /gene="rplV"
                     /locus_tag="ACHKNP_RS12670"
                     /old_locus_tag="pgaptmp_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGU4704679.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHKNP_RS12675"
                     /old_locus_tag="pgaptmp_002535"
     CDS             5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHKNP_RS12675"
                     /old_locus_tag="pgaptmp_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGU4704680.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6101..6511
                     /gene="rplP"
                     /locus_tag="ACHKNP_RS12680"
                     /old_locus_tag="pgaptmp_002536"
     CDS             6101..6511
                     /gene="rplP"
                     /locus_tag="ACHKNP_RS12680"
                     /old_locus_tag="pgaptmp_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGU4704681.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHKNP_RS12685"
                     /old_locus_tag="pgaptmp_002537"
     CDS             6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHKNP_RS12685"
                     /old_locus_tag="pgaptmp_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGU4704682.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHKNP_RS12690"
                     /old_locus_tag="pgaptmp_002538"
     CDS             6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHKNP_RS12690"
                     /old_locus_tag="pgaptmp_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGU4704683.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7121..7492
                     /gene="rplN"
                     /locus_tag="ACHKNP_RS12695"
                     /old_locus_tag="pgaptmp_002539"
     CDS             7121..7492
                     /gene="rplN"
                     /locus_tag="ACHKNP_RS12695"
                     /old_locus_tag="pgaptmp_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGU4704684.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7503..7817
                     /gene="rplX"
                     /locus_tag="ACHKNP_RS12700"
                     /old_locus_tag="pgaptmp_002540"
     CDS             7503..7817
                     /gene="rplX"
                     /locus_tag="ACHKNP_RS12700"
                     /old_locus_tag="pgaptmp_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGU4704685.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7832..8371
                     /gene="rplE"
                     /locus_tag="ACHKNP_RS12705"
                     /old_locus_tag="pgaptmp_002541"
     CDS             7832..8371
                     /gene="rplE"
                     /locus_tag="ACHKNP_RS12705"
                     /old_locus_tag="pgaptmp_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGU4704686.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHKNP_RS12710"
                     /old_locus_tag="pgaptmp_002542"
     CDS             8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHKNP_RS12710"
                     /old_locus_tag="pgaptmp_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGU4704687.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHKNP_RS12715"
                     /old_locus_tag="pgaptmp_002543"
     CDS             8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHKNP_RS12715"
                     /old_locus_tag="pgaptmp_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGU4704688.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9130..9663
                     /gene="rplF"
                     /locus_tag="ACHKNP_RS12720"
                     /old_locus_tag="pgaptmp_002544"
     CDS             9130..9663
                     /gene="rplF"
                     /locus_tag="ACHKNP_RS12720"
                     /old_locus_tag="pgaptmp_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGU4704689.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9673..10026
                     /gene="rplR"
                     /locus_tag="ACHKNP_RS12725"
                     /old_locus_tag="pgaptmp_002545"
     CDS             9673..10026
                     /gene="rplR"
                     /locus_tag="ACHKNP_RS12725"
                     /old_locus_tag="pgaptmp_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGU4704690.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHKNP_RS12730"
                     /old_locus_tag="pgaptmp_002546"
     CDS             10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHKNP_RS12730"
                     /old_locus_tag="pgaptmp_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGU4704691.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHKNP_RS12735"
                     /old_locus_tag="pgaptmp_002547"
     CDS             10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHKNP_RS12735"
                     /old_locus_tag="pgaptmp_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGU4704692.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10731..11165
                     /gene="rplO"
                     /locus_tag="ACHKNP_RS12740"
                     /old_locus_tag="pgaptmp_002548"
     CDS             10731..11165
                     /gene="rplO"
                     /locus_tag="ACHKNP_RS12740"
                     /old_locus_tag="pgaptmp_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGU4704693.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11173..12504
                     /gene="secY"
                     /locus_tag="ACHKNP_RS12745"
                     /old_locus_tag="pgaptmp_002549"
     CDS             11173..12504
                     /gene="secY"
                     /locus_tag="ACHKNP_RS12745"
                     /old_locus_tag="pgaptmp_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGU4704694.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHKNP_RS12750"
                     /old_locus_tag="pgaptmp_002550"
     CDS             12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHKNP_RS12750"
                     /old_locus_tag="pgaptmp_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGU4704695.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHKNP_RS12755"
                     /old_locus_tag="pgaptmp_002551"
     CDS             12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHKNP_RS12755"
                     /old_locus_tag="pgaptmp_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGU4704696.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHKNP_RS12760"
                     /old_locus_tag="pgaptmp_002552"
     CDS             13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHKNP_RS12760"
                     /old_locus_tag="pgaptmp_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGU4704697.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHKNP_RS12765"
                     /old_locus_tag="pgaptmp_002553"
     CDS             13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHKNP_RS12765"
                     /old_locus_tag="pgaptmp_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGU4704698.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHKNP_RS12770"
                     /old_locus_tag="pgaptmp_002554"
     CDS             14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHKNP_RS12770"
                     /old_locus_tag="pgaptmp_002554"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGU4704699.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHKNP_RS12775"
                     /old_locus_tag="pgaptmp_002555"
     CDS             15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHKNP_RS12775"
                     /old_locus_tag="pgaptmp_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGU4704700.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15761..16129
                     /locus_tag="ACHKNP_RS12780"
                     /old_locus_tag="pgaptmp_002556"
     CDS             15761..16129
                     /locus_tag="ACHKNP_RS12780"
                     /old_locus_tag="pgaptmp_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HGU4704701.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16140..16565
                     /gene="zntR"
                     /locus_tag="ACHKNP_RS12785"
                     /old_locus_tag="pgaptmp_002557"
     CDS             16140..16565
                     /gene="zntR"
                     /locus_tag="ACHKNP_RS12785"
                     /old_locus_tag="pgaptmp_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HGU4704702.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16621..16839
                     /gene="arfA"
                     /locus_tag="ACHKNP_RS12790"
                     /old_locus_tag="pgaptmp_002558"
     CDS             16621..16839
                     /gene="arfA"
                     /locus_tag="ACHKNP_RS12790"
                     /old_locus_tag="pgaptmp_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HGU4704703.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHKNP_RS12795"
                     /old_locus_tag="pgaptmp_002559"
     CDS             complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHKNP_RS12795"
                     /old_locus_tag="pgaptmp_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGU4704704.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHKNP_RS12800"
                     /old_locus_tag="pgaptmp_002560"
     CDS             complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHKNP_RS12800"
                     /old_locus_tag="pgaptmp_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGU4704705.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHKNP_RS12805"
                     /old_locus_tag="pgaptmp_002561"
     CDS             complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHKNP_RS12805"
                     /old_locus_tag="pgaptmp_002561"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGU4704706.1"
                     /translation="MKKQRNLRSMAAQAVEQIVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGVYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAGFYASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHKNP_RS12810"
                     /old_locus_tag="pgaptmp_002562"
     CDS             complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHKNP_RS12810"
                     /old_locus_tag="pgaptmp_002562"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGU4704707.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHKNP_RS12815"
                     /old_locus_tag="pgaptmp_002563"
     CDS             complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHKNP_RS12815"
                     /old_locus_tag="pgaptmp_002563"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGU4704708.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21710..22834
                     /gene="dprA"
                     /locus_tag="ACHKNP_RS12820"
                     /old_locus_tag="pgaptmp_002564"
     CDS             21710..22834
                     /gene="dprA"
                     /locus_tag="ACHKNP_RS12820"
                     /old_locus_tag="pgaptmp_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HGU4704709.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22806..23280
                     /gene="smg"
                     /locus_tag="ACHKNP_RS12825"
                     /old_locus_tag="pgaptmp_002565"
                     /pseudo
     CDS             22806..23280
                     /gene="smg"
                     /locus_tag="ACHKNP_RS12825"
                     /old_locus_tag="pgaptmp_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
     gene            23309..23851
                     /locus_tag="ACHKNP_RS12830"
                     /old_locus_tag="pgaptmp_002566"
     CDS             23309..23851
                     /locus_tag="ACHKNP_RS12830"
                     /old_locus_tag="pgaptmp_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HGU4704710.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23856..24428
                     /gene="tsaC"
                     /locus_tag="ACHKNP_RS12835"
                     /old_locus_tag="pgaptmp_002567"
     CDS             23856..24428
                     /gene="tsaC"
                     /locus_tag="ACHKNP_RS12835"
                     /old_locus_tag="pgaptmp_002567"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HGU4704711.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24433..25251
                     /gene="aroE"
                     /locus_tag="ACHKNP_RS12840"
                     /old_locus_tag="pgaptmp_002568"
     CDS             24433..25251
                     /gene="aroE"
                     /locus_tag="ACHKNP_RS12840"
                     /old_locus_tag="pgaptmp_002568"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGU4704712.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25248..25505
                     /locus_tag="ACHKNP_RS12845"
                     /old_locus_tag="pgaptmp_002569"
     CDS             25248..25505
                     /locus_tag="ACHKNP_RS12845"
                     /old_locus_tag="pgaptmp_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HGU4704713.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25481..26035)
                     /locus_tag="ACHKNP_RS12850"
                     /old_locus_tag="pgaptmp_002570"
     CDS             complement(25481..26035)
                     /locus_tag="ACHKNP_RS12850"
                     /old_locus_tag="pgaptmp_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HGU4704714.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6817 a         6295 c         6864 g         6527 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gttgctaaag ttctgggcta attacacgtt
      241 aattagtttt gaattgaaaa gggcgcttcg gcgccctttt tgctgcccgt aatttgagct
      301 cgaaatattt agtcgtaatt ttgctcgctg aactatggcg caaataaaac tgtgctattg
      361 taatcataac cattctcatt tacactttgc gcagaatttg aacgggagca atcatgtacg
      421 tttgtttgtg taatggtgta agtgacaaaa aaattcgcca ggcagtccgt cagtttcatc
      481 cgcaatcttt ccaacaattg cgtaagttta ttcctgtggg aaatcaatgc ggtaagtgca
      541 ttcgcgccgc gcgcgaagtg atgcaggacg agttgatgca gatgccagaa tttaaagaga
      601 ttgcctgaag accacgctta ttttttgaca tcccgctagc cccatctacg ctctaatagt
      661 ggaagcggag ggactataaa atgaaaggtg atgttaaaat cataaattat ctcaataaac
      721 tattgggaaa tgagcttgtc gcaatcaatc agtattttct ccatgcccgg atgtttaaaa
      781 actggggcct gatgcgtctc aatgacgtcg aataccatga atccatcgat gagatgaaac
      841 acgctgataa atatatcgag cgtattttgt ttttagaagg aatccccaac ttacaggatt
      901 taggcaaact ggggattggt gaagatgtcg aggagatgct gcaatccgac ctgcgattag
      961 aactggaagg cgcgaaggat ctccgtgagg ctatcgcgta tgccgacagc gttcatgact
     1021 atgttagccg tgatatgatg atcgagatcc ttgccgatga agaaggtcat attgactggc
     1081 tcgaaactga gctggatctc atcggtaaaa tcggtttaca aaattacctg caatcacaaa
     1141 taaaagttaa agattaacgg cctgctagca aactatccca acctacaacg aaacccgcag
     1201 ccgccagaaa tggcccaaac ggcagcgggt tttttaatga ctcttttcct ctcatgagca
     1261 gaccaacaac tacggctccg caagcgaatg aggcagcaag gaaaaccagt cgtggcaaga
     1321 aggtccaggt atgccaggca ccaagggcgg cgagaaactt cacatcacca tagcctaatc
     1381 cttctttatg acgcagtatg cgataccccc agtaaatgac agcgaatgtg ccgtaaccaa
     1441 taatcgcccc ccataacgca tcggctaaac cgtcaggatt acaaacctga gagaacaaaa
     1501 gacctgacca gagtaacggg caggtaaatc tgtcaggtaa caagccatgc tttgcatccc
     1561 ataaaaaaag taggacagat aaacaagcgt acaaaatcag aaagggaagg ttagcgtcca
     1621 taaaacatcc tctgtaattg gagtatcacc atcgaataac tccggatgct cagcaacaga
     1681 gaaatcgctg atttgttaac ctggttccgg tgaaaaacgc tatcttcaca accttacaaa
     1741 tttcgtggaa tacagctcgc aatctgcgcg ttgcgattaa tttctacccg atgcttgcgt
     1801 caggctcaga tttgcgtata atgcgcgggc ttgtcgtagt tgacagcagg ttcaatctga
     1861 acctcagtta gccgaatttg gctacctaac aatgctccca atcggggagc tacgtaagaa
     1921 cggttacact ctcccatcaa tcgtaatggg tctgaggagt aatcattttc gtttataaaa
     1981 taattggagc tctggtctca tgcagaacca aagaatccgt atccgcctga aagcgtttga
     2041 tcatcgtctg atcgatcaag caaccgcgga aatcgtcgag actgccaagc gcactggtgc
     2101 gcaggtccgt ggtccgatcc cgctgccgac acgcaaagag cgcttcactg ttctgatctc
     2161 cccgcacgtc aacaaagacg cgcgcgatca gtacgaaatc cgtactcact tgcgtctggt
     2221 tgacatcgtt gagccaaccg agaaaaccgt tgatgctctg atgcgtctgg atctggctgc
     2281 cggtgtagac gtgcagatca gcctgggtta atcaggtcat tgagcgattg agaggttgaa
     2341 acaatgattg gtttagtcgg taaaaaagtg ggtatgaccc gtatcttcac agaagacggc
     2401 gtttctatcc cagtaaccgt aatcgaagtt gaagcaaacc gcgttactca ggttaaagac
     2461 ctggctaacg atggctaccg tgctattcag gtgaccaccg gtgctaaaaa agctaaccgt
     2521 gtgaccaagc ctgaagctgg ccacttcgct aaagctggcg tagaagctgg ccgtggtctg
     2581 tgggaattcc gcctggctga aggcgaagag ttcactgtag gtcagagcat tagcgttgaa
     2641 ctgtttgctg acgttaaaaa agttgacgta actggcacct ctaaaggtaa aggtttcgca
     2701 ggtaccgtta agcgctggaa cttccgtacc caggacgcta ctcacggtaa ctccttgtct
     2761 caccgcgttc cgggttctat cggtcagaac cagactccgg gcaaagtgtt caaaggcaag
     2821 aaaatggcag gtcagatggg taacgaacgt gtaaccgttc agagccttga cgtagtacgc
     2881 gttgacgctg agcgcaacct gctgctggtt aaaggtgctg tcccgggtgc aaccggtagc
     2941 gacctgatcg ttaaaccagc tgtgaaggcg taaggagata gcaatggaat tagtattgaa
     3001 agacgcgcag agcgcgctga ctgtttccga aactaccttc ggtcgtgatt tcaacgaagc
     3061 gctggttcac caggttgttg ttgcttatgc agctggtgct cgtcagggta ctcgtgctca
     3121 gaagactcgt gctgaagtaa ctggttctgg taaaaaaccg tggcgccaga aaggcaccgg
     3181 ccgtgcgcgt tctggttcta tcaagagccc gatctggcgt tctggtggcg tgacctttgc
     3241 tgctcgtccg caggaccaca gtcaaaaagt taacaagaag atgtaccgcg gcgcgctgaa
     3301 aagcatcctg tccgaactgg tacgtcagga tcgtctgatc gttgtcgaga agttctctgt
     3361 agaagcgccg aaaactaagc tgctggcaca gaaactgaaa gacatggctc tggaagatgt
     3421 gctgatcatc accggtgagc tggacgaaaa cctgttcctg gctgcgcgca acctgcacaa
     3481 ggttgacgta cgcgatgcaa ctggtatcga cccggttagc ctgatcgcct tcgacaaagt
     3541 cgtaatgact gctgatgctg ttaagcaagt tgaggagatg ctggcatgat tcgtgaagaa
     3601 cgtctgctga aggtgctgcg tgcaccgcac gtttctgaaa aagcgtctac tgcgatggaa
     3661 aaatccaaca ccatcgtact caaagttgct aaagacgcga ccaaagcaga aatcaaagct
     3721 gctgtgcaga aactgtttga agtcgaagtc gaagtcgtta acaccctggt agttaaaggg
     3781 aaagttaaac gtcacggaca gcgtatcggt cgtcgtagcg actggaaaaa agcttacgtc
     3841 accctgaaag aaggccagaa tctggacttc gttggcggcg ctgagtaagt cggaggagta
     3901 atacaatggc agttgttaaa tgtaaaccga catctccggg tcgtcgccac gtagttaaag
     3961 tggttaaccc tgagctgcac aagggcaaac cttttgctcc gttgctggaa aaaaacagca
     4021 aatccggtgg tcgtaacaac aatggccgta tcaccactcg tcatatcggt ggtggccaca
     4081 agcaggctta ccgtattgtt gacttcaaac gcaacaaaga cggtatcccg gcagttgttg
     4141 aacgtcttga gtacgatccg aaccgttccg cgaacatcgc gctggttctg tacaaagacg
     4201 gtgaacgccg ttacatcctg gcccctaaag gcctgaaagc tggcgaccag attcagtctg
     4261 gcgttgatgc tgcaatcaaa ccaggtaaca ccctgccgat gcgcaacatc ccggttggtt
     4321 ctactgttca taacgtagaa atgaaaccag gtaaaggcgg tcagctggca cgttccgctg
     4381 gtacttacgt tcagatcgtt gctcgtgatg gtgcttatgt caccctgcgt ctgcgttctg
     4441 gtgaaatgcg taaagtagaa gcagactgcc gtgcaactct gggcgaagtt ggcaatgctg
     4501 agcatatgct gcgcgttctg ggtaaagcag gtgctgcacg ctggcgtggt gttcgtccga
     4561 ccgttcgcgg taccgcgatg aacccggtag accacccaca tggtggtggt gaaggtcgta
     4621 actttggtaa gcacccggta actccgtggg gcgttcagac caaaggtaag aagacccgca
     4681 gcaacaagcg tactgataaa ttcatcgtac gtcgccgtag caaataattt tagaggataa
     4741 gccatgccac gttctctcaa gaaaggtcct tttattgacc tgcacttgct gaagaaggta
     4801 gagaaagcgg tggaaagcgg agacaagaag cccctgcgca cttggtcccg tcgttcaacg
     4861 atctttccta acatgatcgg tttgaccatc gctgtccata atggtcgtca gcacgttccg
     4921 gtatttgtaa ccgacgaaat ggtcggtcac aaactgggtg aattcgcacc gactcgtact
     4981 tatcgcggcc acgctgctga taaaaaagcg aagaagaaat aaggtaggag gaagagatgg
     5041 aaactatcgc taaacatcgc catgctcgtt cttctgctca gaaggttcgc cttgttgctg
     5101 acctgattcg cggtaagaaa gtgtcgcagg ctctggatat tttgacctac accaacaaga
     5161 aagcggctgt actggtcaag aaagttctgg aatctgccat tgctaacgct gaacacaacg
     5221 atggcgctga cattgacgat ctgaaagtta cgaaaatttt cgtagacgaa ggcccgagca
     5281 tgaagcgcat tatgccgcgt gcaaaaggtc gtgcagatcg catcctgaag cgcaccagcc
     5341 acatcactgt ggttgtgtcc gatcgctgag actctggaga ctagcaatgg gtcagaaagt
     5401 acatcctaat ggtattcgcc tgggtattgt aaaaccatgg aactctacct ggtttgcgaa
     5461 caccaaagaa ttcgctgaca acctggacag cgattttaaa gtacgtcagt acctgactaa
     5521 ggaactggct aaagcgtccg tatctcgtat cgttatcgag cgtccggcta agagcatccg
     5581 tgtaaccatt cacactgctc gcccgggtat cgttatcggt aaaaaaggtg aagacgtaga
     5641 aaaactgcgt aaggtcgtag cggacatcgc tggcgttcct gcacagatca acatcgccga
     5701 agttcgtaag cctgaactgg acgcaaaact ggttgctgac agcatcactt ctcagctgga
     5761 acgtcgcgtt atgttccgtc gtgctatgaa gcgtgctgta cagaacgcaa tgcgtctggg
     5821 cgctaaaggt attaaagttg aagttagcgg ccgtctgggc ggcgcggaaa tcgcacgtac
     5881 cgaatggtac cgcgaaggtc gcgtaccgct gcacactctg cgtgctgaca tcgactacaa
     5941 cacctctgaa gcgcacacca cttacggtgt aatcggcgtt aaagtgtgga tcttcaaagg
     6001 cgagatcctg ggtggtatgg ctgctgttga acaaccggaa aaaccggctg ctcagcctaa
     6061 aaagcagcag cgtaaaggcc gtaaataagg agcgtcgctg atgttacaac caaagcgtac
     6121 aaaattccgt aaaatgcaca aaggccgtaa ccgcggtctg gcgcagggta cggatgttag
     6181 cttcggcagc ttcggtctga aagctgttgg ccgtggtcgt ctgactgccc gtcagatcga
     6241 agcagcacgt cgtgctatga cccgtgcagt taagcgtcaa ggtaagatct ggatccgtgt
     6301 gttcccggac aaaccgatca ctgaaaagcc gctggcagtg cgtatgggta aaggtaaagg
     6361 taacgtggag tattgggttg ccttgattca gccgggtaaa gtcctgtatg aaatggacgg
     6421 tgttccggaa gagctggccc gtgaagcatt caagctggca gcagcgaaac tgccgattaa
     6481 aaccaccttt gtaactaaga cggtgatgta atgaaagcaa aagagctgcg tgagaagagc
     6541 gttgaagagc tgaacaccga gctgctgaac ctgctgcgtg agcagttcaa cctgcgtatg
     6601 caggctgcaa gtggccagct gcaacagtct cacctgttga agcaagtgcg tcgcgatgtc
     6661 gcacgcgtta agactttact gaacgagaag gcgggtgcgt aatgaccgat aaaatccgta
     6721 ctctgcaagg tcgcgttgtt agcgacaaaa tggagaaatc cattgttgtt gctatcgaac
     6781 gttttgtgaa acacccgatc tacggtaaat tcatcaagcg tacgaccaaa ctgcacgtac
     6841 atgacgagaa caacgaatgc ggtatcggtg acgtggttga aatccgcgaa tgccgtccgc
     6901 tgtccaagac taaatcctgg acgctggttc gcgttgtaga gaaagcggtt ctgtaataca
     6961 gtacactctc tcgatacgaa taaacggctc agaaatgagc cgtttatttt ttctacccat
     7021 atccttgaag cggtgttata atgccgcgcc ctcgatatgg ggatttttaa cgacctgatt
     7081 ttcgggtctc agtagtagtt gacattagcg gagcactaaa atgatccaag aacagactat
     7141 gctgaacgtc gccgacaact ccggtgcacg tcgcgtaatg tgtatcaagg ttctgggtgg
     7201 ctcgcaccgt cgctacgcag gcgtaggcga catcatcaag atcaccatca aagaagcaat
     7261 tccgcgtggt aaggtcaaaa aaggtgatgt gctgaaggcg gtagtggtgc gcaccaagaa
     7321 gggtgttcgt cgcccggacg gttctgtcat tcgcttcgat ggtaatgctt gtgttcttct
     7381 gaacaacaac agcgagcagc ctatcggtac gcgtattttt gggccggtaa ctcgtgagct
     7441 tcgtagtgag aagttcatga aaattatctc tctggcacca gaagtactct aaggagcgaa
     7501 tcatggcagc gaaaatccgt cgtgatgacg aagttatcgt gttaaccggt aaagataaag
     7561 gtaaacgcgg taaagttaag aatgtcctgt cttccggcaa ggtcattgtt gaaggtatca
     7621 acctggttaa gaaacatcag aagccggttc cggccctgaa ccaaccgggt ggcatcgttg
     7681 aaaaagaagc cgctattcag gtttccaacg tagcaatctt caatgcggca accggcaagg
     7741 ctgaccgtgt aggctttaga ttcgaagacg gcaaaaaagt ccgtttcttc aagtctaaca
     7801 gcgaaactat caagtaattt ggagtagtac gatggcgaaa ctgcatgatt actacaaaga
     7861 cgaagtagtt aaaaaactca tgactgagtt taactacaat tctgtcatgc aagtccctcg
     7921 ggtcgagaag atcaccctga acatgggtgt tggtgaagcg atcgctgaca aaaaactgct
     7981 ggataacgca gcagcagacc tggcagcaat ctccggtcaa aaaccgctga tcaccaaagc
     8041 acgcaaatct gttgcaggct tcaaaatccg tcagggctat ccgatcggct gtaaagtaac
     8101 tctgcgtggc gaacgcatgt gggagttctt tgagcgcctg atcactattg ctgtacctcg
     8161 tatccgtgac ttccgtggcc tgtccgctaa gtctttcgac ggtcgtggta actacagcat
     8221 gggtgtccgt gagcagatca tcttcccaga aatcgactac gataaagtcg accgcgttcg
     8281 tggtttggat attaccatta ccactactgc gaaatctgac gaagaaggcc gcgctctgct
     8341 ggctgccttt gacttcccgt tccgcaagta aggtagggtt actaaatggc taagcaatca
     8401 atgaaagcac gcgaagtaaa acgcgtagct ttagctgata aatacttcgc gaaacgcgct
     8461 gaactgaaag cgatcatctc tgatgtgaac gcttccgacg aagatcgttg gaacgctgtt
     8521 ctcaagctgc agactctgcc gcgtgattcc agcccgtctc gtcagcgtaa ccgctgccgt
     8581 caaacaggtc gtccgcatgg tttcctgcgg aagttcgggt tgagccgtat taaggtccgt
     8641 gaagccgcta tgcgcggtga aatcccgggt ctgaaaaagg ctagctggta attgtcacca
     8701 attgaatcac gggagtaaag acagatgagc atgcaagatc cgatcgcgga tatgctgacc
     8761 cgtatccgta acggtcaggc cgcgaacaaa gctgcggtca ccatgccttc ctccaagctg
     8821 aaagtggcaa tcgccaacgt gctgaaggaa gaaggtttta ttgaagattt taaagttgaa
     8881 ggcgacacca agcctgaact ggaacttact ctgaagtatt tccagggcaa agctgttgta
     8941 gaaagcattc agcgtgtcag ccgcccaggt ctgcgcatct ataaacgtaa agatgagctg
     9001 ccgaaagtta tggcgggtct gggtatcgca gttgtttcta cctctaaagg tgttatgact
     9061 gatcgtgcag cgcgccaggc tggtcttggt ggcgaaatta tctgctacgt agcctaatcg
     9121 gaggaaaaaa tgtctcgtgt tgctaaagca ccggtcgttg ttcctgccgg cgttgacgta
     9181 aaaatcaacg gtcaggttat tacgatcaaa ggtaaaaacg gcgagctgac tcgtactctc
     9241 aacgatgctg ttgaagttaa acatgcagat aataccctga ccttcggtcc gcgtgatggt
     9301 tacgcagacg gttgggcaca ggctggtacc gcgcgtgccc tgctgaactc aatggttatc
     9361 ggtgttaccg aaggcttcac taagaagctg cagctggttg gtgtaggtta ccgtgcagcg
     9421 gttaaaggca atgtgattaa cctgtctctg ggtttctctc atcctgttga ccatcagctg
     9481 cctgcgggta tcactgctga atgtccgact cagactgaaa tcgtgctgaa aggcgctgat
     9541 aagcaggtga tcggccaggt tgcagcggat ctgcgcgcct accgtcgtcc tgagccttat
     9601 aaaggcaagg gtgttcgtta cgccgacgaa gtcgtgcgta ccaaagaggc taagaagaag
     9661 taaggtaaca ctatggataa gaaatctgct cgtatccgtc gtgcgacccg cgcacgccgc
     9721 aagctccagg agctgggcgc aactcgcctg gtggtacatc gtaccccgcg tcacatttac
     9781 gcacaggtaa ttgcaccgaa cggttctgaa gttctggtag ctgcttctac tgtagaaaaa
     9841 gctatcgctg aacaactgaa gtacaccggt aacaaagacg cggctgcagc tgtgggtaaa
     9901 gctgtcgctg aacgcgctct ggaaaaaggc atcaaagatg tatcctttga ccgttccggg
     9961 ttccaatatc atggtcgtgt ccaggcactg gcagatgctg cccgtgaagc tggccttcag
    10021 ttctaaggta gaggtgtaag atggctcaca tcgaaaaaca agctggcgaa ctgcaggaaa
    10081 agctgatcgc ggtaaaccgc gtatctaaaa ccgttaaagg tggtcgtatt ttctccttca
    10141 cagctctgac tgtagttggc gatggtaacg gtcgcgttgg ttttggttac ggtaaagcgc
    10201 gtgaagttcc agcagcgatc cagaaagcga tggaaaaagc ccgtcgcaat atgattaacg
    10261 tcgcgctgaa taacggcact ctgcaacacc ctgttaaagg tgttcacacg ggttctcgcg
    10321 tattcatgca gccggcttcc gaaggtaccg gtatcatcgc cggtggtgca atgcgcgccg
    10381 ttctggaagt cgctggggtt cataacgttc tggctaaagc atatggttcc accaacccga
    10441 tcaacgtggt tcgtgcaact attgatggcc tggaaaatat gaattctcca gaaatggtcg
    10501 ctgccaagcg tggtaaatcc gttgaagaaa ttctggggaa ataaaccatg gcaaagacta
    10561 ttaaaattac tcaaacccgc agtgcaatcg gtcgtctgcc gaaacacaag gcaacgctgc
    10621 ttggcctggg tctgcgtcgt attggtcaca ccgtagagcg cgaggatact cctgctattc
    10681 gcggtatgat caacgcggtt tccttcatgg ttaaagttga ggagtaagag atgcgtttaa
    10741 atactctgtc tccggccgaa ggctccaaaa aggcgggtaa acgcctgggt cgtggtatcg
    10801 gttctggcct cggtaaaacc ggtggtcgtg gtcacaaagg tcagaaatct cgttctggcg
    10861 gtggcgtacg tcgcggtttc gagggtggtc agatgcctct gtaccgtcgt ctgccgaaat
    10921 tcggcttcac ttctcgtaaa gcagcgatta cagccgaagt tcgtctgtct gacctggcta
    10981 aagtagaagg cggtgttgta gacctgaaca cgctgaaagc ggctaacatt atcggtatcc
    11041 agatcgagtt cgcgaaagtg atcctggctg gcgaagtaac gactccggta actgttcgtg
    11101 gcctgcgtgt tactaaaggc gctcgtgctg ctatcgaagc tgctggcggt aaaatcgagg
    11161 aataagtagc agatggctaa acaaccggga ttagattttc aaagtgccaa aggtggctta
    11221 ggcgagctga aacgcagact gctgtttgtt atcggtgcgc tgattgtgtt ccgtattggc
    11281 tcttttattc cgatccctgg tattgatgcc gctgtacttg ccaaactgct tgagcaacag
    11341 cgaggcacca tcattgagat gtttaacatg ttctctggtg gtgctctcag ccgtgcttct
    11401 atctttgctc tggggatcat gccgtatatt tcggcgtcga tcattatcca gctgctgacg
    11461 gtagttcacc caacgttggc agaaattaag aaagaagggg agtctggtcg tcgtaagatc
    11521 agccagtaca cccgttacgg tactctggtg ctggcaatat tccagtcgat cggtattgct
    11581 accggtctgc cgaatatgcc tggtatgcaa ggcctggtga ttaacccagg ctttgcattc
    11641 tacttcaccg ctgttgtaag tctggtcaca ggaacgatgt tcctgatgtg gttgggcgaa
    11701 cagattactg aacgaggtat cggcaacggt atttcaatca ttatcttcgc cggtattgtc
    11761 gcgggactcc cgccagccat tgcccatact atcgagcaag cgcgtcaagg cgacctgcac
    11821 ttcctcgtgt tgctgttggt tgcagtatta gtatttgcag tgacgttctt tgttgtattt
    11881 gttgagcgtg gtcaacgccg cattgtggta aactacgcga aacgtcagca aggtcgtcgt
    11941 gtctatgctg cacagagcac acatttaccg ctgaaagtga atatggcggg ggtaatcccg
    12001 gcaatcttcg cttccagtat tattctgttc ccggcgacca tcgcgtcatg gttcgggggc
    12061 ggtactggtt ggaactggct gacaacaatt tcgctgtatt tgcagcctgg gcaaccgctt
    12121 tatgtgttac tctatgcgtc tgcaatcatc ttcttctgtt tcttctacac ggcgttggtt
    12181 ttcaacccgc gtgaaacagc agataacctg aagaagtccg gtgcatttgt accaggaatt
    12241 cgtccgggag agcaaacggc gaagtatatc gataaagtaa tgacccgcct gaccctggtt
    12301 ggtgcgctgt atattacctt tatctgcctg atcccggagt tcatgcgtga tgcaatgaaa
    12361 gtaccgttct acttcggtgg gacctcactg cttatcgttg ttgtcgtgat tatggacttt
    12421 atggctcaag tgcaaactct gatgatgtcc agtcagtatg agtctgcatt gaagaaggcg
    12481 aacctgaaag gctacggccg ataattggtc gcccgagaag ttacggagag taaaaatgaa
    12541 agttcgtgct tccgtcaaga aattatgccg taactgcaaa atcgttaagc gtgatggtgt
    12601 catccgtgtg atttgcagtg ccgagccgaa gcataaacag cgccaaggct gattttttcg
    12661 catatttttc ttgcaaagtt gggttgagct ggctagatta gccagccaat cttttgtatg
    12721 tctgtgcgtt tccatttgag tatcctgaaa acgggctttt cagcatggaa cgtacatatt
    12781 aaatagtagg agtgcatagt ggcccgtata gcaggcatta acattcctga tcataagcat
    12841 gccgtaatcg cattaacttc gatttatggc gtcggcaaga cccgttctaa agccatcctg
    12901 gctgcagcgg gtatcgctga agatgttaag atcagtgagc tgtctgaagg acaaatcgac
    12961 acgctgcgtg acgaagttgc caaatttgtc gttgaaggtg atctgcgccg tgaaatcagc
    13021 atgagcatca agcgcctgat ggatcttggt tgctatcgcg gtttgcgtca tcgtcgtggt
    13081 ctcccggttc gcggtcagcg taccaagacc aacgcacgta cccgtaaggg tccgcgcaaa
    13141 ccgatcaaga aataatcggg gtgattgaat aatggcaaag gcaccaattc gtgcacgtaa
    13201 acgtgtaaga aaacaagtct ctgacggcgt ggctcatatc catgcttctt tcaacaacac
    13261 catcgtgact atcactgatc gtcagggtaa cgcgttgggt tgggcaacag ccggtggttc
    13321 cggtttccgt ggttctcgca aatccactcc gtttgcagct caggttgcag cagagcgttg
    13381 cgctgacgcc gtgaaagaat acggcatcaa gaatctggaa gttatggtta aaggtccggg
    13441 tccaggccgc gaatctacta ttcgtgctct gaacgccgca ggtttccgca tcactaacat
    13501 tactgatgtg actccgatcc ctcataacgg ttgtcgtccg ccgaaaaaac gtcgcgtata
    13561 acgcctcgtt ttccaggttt gttggagaaa gaaaatggca agatatttgg gtcctaagct
    13621 caagctgagc cgtcgtgagg gcaccgactt attccttaag tctggcgttc gcgcgatcga
    13681 taccaagtgt aaaattgaac aagctcctgg ccagcacggt gcgcgtaaac cgcgtctgtc
    13741 tgactatggt gtgcagttgc gtgaaaagca aaaagttcgc cgtatctatg gtgtgctgga
    13801 gcgtcagttc cgtaactact acaaagaagc agcacgtctg aaaggcaaca ccggtgaaaa
    13861 cctgttggct ctgctggaag gtcgtctgga caacgttgta taccgtatgg gcttcggtgc
    13921 cactcgtgca gaagcacgtc agctggttag ccataaagca attatggtaa acggtcgtgt
    13981 tgttaacatc gcttcttatc aggttagtcc gaatgacgtt gtaagcattc gtgagaaagc
    14041 gaagaagcag tctcgcgtga aagccgctct ggagctggct gagcagcgtg aaaagccaac
    14101 ctggctggaa gttgatgctg gcaagatgga aggtacgttt aagcgtaagc cggagcgttc
    14161 tgatctgtct gcggacatta acgaacacct gatcgtcgag ctttactcca agtaaagctt
    14221 agtaccaaag agaggacaca atgcagggtt ctgtgacaga gtttctaaaa ccgcgcctgg
    14281 ttgatatcga gcaagtgagt tcgacgcacg ccaaggtgac ccttgagcct ttagagcgtg
    14341 gctttggcca tactctgggt aacgcactgc gccgtattct gctctcatcg atgccgggtt
    14401 gcgcggtgac cgaggttgag attgatggtg tactacatga gtacagcacc aaagaaggcg
    14461 ttcaggaaga tatcctggaa atcctgctca acctgaaagg gctggcggtg agagttcagg
    14521 gcaaagatga agttattctt accttgaata aatctggcat tggccctgtg actgcagccg
    14581 atatcaccca cgacggtgat gtcgaaatcg tcaagccgca gcacgtgatc tgccacctga
    14641 ccgatgagaa cgcgtctatt agcatgcgta tcaaagttca gcgcggtcgt ggttatgtgc
    14701 cggcttctac ccgaattcat tcggaagaag atgagcgccc aatcggccgt ctgctggtcg
    14761 acgcatgcta cagccctgtg gagcgtattg cctacaatgt tgaagcagcg cgtgtagaac
    14821 agcgtaccga cctggacaag ctggtcatcg aaatggaaac caacggcaca atcgatcctg
    14881 aagaggcgat tcgtcgtgcg gcaaccattc tggctgaaca actggaagct ttcgttgact
    14941 tacgtgatgt acgtcagcct gaagtgaaag aagagaaacc agagttcgat ccgatcctgc
    15001 tgcgccctgt tgacgatctg gaattgactg tccgctctgc taactgcctt aaagcagaag
    15061 ctatccacta tatcggtgat ctggtacaac gtaccgaggt tgagctcctt aaaacgccta
    15121 accttggtaa aaaatctctt actgagatta aagacgtgct ggcttcccgt ggactgtctc
    15181 tgggcatgcg cctggaaaac tggccaccgg caagcatcgc tgacgagtaa ccggatcaca
    15241 ggttaaggtt ttactgagaa ggataaggtc atgcgccatc gtaagagtgg tcgtcaactg
    15301 aaccgcaaca gcagccatcg ccaggctatg ttccgcaata tggcaggttc actggttcgt
    15361 catgaaatca tcaagacgac tctgcctaaa gcgaaagagc tgcgccgcgt agttgagccg
    15421 ctgattactc ttgccaagac tgatagcgtt gctaatcgtc gtctggcatt cgcccgtact
    15481 cgtgataacg agatcgtggc aaaactgttt aacgaactgg gcccgcgttt cgcgagccgt
    15541 gccggtggtt acactcgtat tctgaagtgt ggcttccgtg caggcgacaa cgcgccgatg
    15601 gcttacatcg agctggttga tcgttcagag aaagcagaag ctgctgcaga gtaatctgaa
    15661 gcaacgtaaa aaaacccgcc ccggcgggtt tttttatacc cgtagcatcc ccacttatct
    15721 acaatagctg tactcttttt gttcatcccc tggagtattt atgtggttac ttgaccagtg
    15781 ggcagagcgt catatagcag aagcgcaagc gaaaggtgag tttgataacc tggcaggtag
    15841 cggcgaacca ttgatactgg atgatgattc tcatgtgcca ccggaattac gtgcggggta
    15901 tcgcttgctg aagaatgccg gttgcttacc gccagaactt gagcagcgga gggaagcaat
    15961 tcagcttctg gatatcctga gaggtatccg tcacgatgat ccgcaatatc aagaggttag
    16021 ccgtcgattg tcattactgg aattgaagct gcgacaagct ggattgagta ccgatttttt
    16081 acgcggcgat tatgctgaca agttgttgga caaaatcaac gataactagt ggagtatgta
    16141 tgtatcgcat tggtgagctg gcaaaaatgg cggaagtaac acctgacacg attcgttatt
    16201 acgaaaaaca gcagatgatg gagcatgaag tgcgtactga aggcgggttt cgcctgtata
    16261 ccgaaagcga tctccagcga ttgaaattta tccgccatgc cagacaacta ggtttcagtc
    16321 tggagtcgat ccgcgagttg ctgtcgatcc gcatcgatcc tgaacaccat acctgtcagg
    16381 agtcaaaagg cattgtgcag gaaagattgc aggaagtcga agcacggata gccgagttgc
    16441 agagtatgca gcgttccttg caacgcctta acgatgcctg ttgtgggacc gcccatagca
    16501 gtgtttattg ttcgattctt gaagctcttg aacaaggggc gagtggcgtt aagagtggct
    16561 gttgattttt tgcactgaca ggattacact cgcgccgtaa aataaccaac tggagttttt
    16621 atgagccgat atcagcatac taaagggcag ataaaggata atgcgataga agcattacta
    16681 catgatccct tattccgaca gcgagtagag aaaaataaga aagggaaagg cagttacatg
    16741 cgaaaaggaa aacatggcaa tcgggggaac tgggaggcca gtggcaagaa agtaaatcac
    16801 ttttttacca ctggtcttct gctttcaggc gcttgttaag agcggttatt ctgctctttc
    16861 agcaaatcac gaatttctgc cagtaatact tcttctttag ttggtgcagg tgcggcagct
    16921 ggttcttctt ttttccgatt cagtttgttg attagcttaa tcgccataaa gatggcaaag
    16981 gccacaatca gaaaatcaaa gacgttttga atgaagacac cgtaatgcat cacaacagca
    17041 gggatatccc cctgcgcatc gcgtagcgtg acagcaaact gtttaaaatc gatcccgcca
    17101 attaataagc ccagaggagg catgatgata tcggcaacca gtgaagagac aatcttcccg
    17161 aatgccgcac cgataatgac acccaccgcc aaatccacca cgttcccgcg catcgcaaat
    17221 tcgcgaaatt ctttaataat gctcatgtta ttctccctat gaagccgaca accataagtc
    17281 taacaaatgt taagccattt tcctgccagg aatgaattta attaatcctt gataatcaaa
    17341 gggaaataat aaggcgtcat tagacgcctt attaattaca agaagaaagg gcttggttgg
    17401 aagagtcttt cgacgtcggt aataaacttt ttatctgtga ggaacataat tacgtgatcg
    17461 ccttgctcaa tgcgcagatt gtcattggca atcatcacgt cgtttccacg taccaccgct
    17521 ccaataatcg ttcctggcgg tagcttgatt tcgtcaatga ctctgccgac aacgcgtgag
    17581 gtgctttcat caccgtgagc aacggcttca atagcttctg ctacgccgcg gcgcaatgag
    17641 gaaacaccaa caatatctgc ttttcgcaca tggctaagca acgcagaaat agttgcttgt
    17701 tgtggtgaaa tcgcaatatc gatcacactc ccctgaacca gatccacata agcgcgacgc
    17761 tggatcaata ccatcacctt tttcgcaccc atacgtttgg caagcatggc ggacatgata
    17821 ttggcctcgt catcgttggt gacggcaata aacagatcaa cttgatcgat atgttcttcg
    17881 gccagcagtt cttgatccga cgcatcacca aaaaagacga tcgtattctg caacttttcc
    17941 gccagttcgg cagcgcgctg ctgattacgt tcgatgagtt taacgctgta atctttttcc
    18001 agacgacgcg ccagccctgc accgatatta ccgccaccaa ccagcatgat ccgcttatac
    18061 ggtttttcca gtcgctgtaa ttcactcatc accgcacgga tatgctgtga agcggcaata
    18121 aagaacactt catcaccagc ttccacaatg gtcgaacctt gtggacgaat ggggcgatcg
    18181 tggcggaaaa ttgctgccac acgagtatcg atatgtggca tatgttcgcg catggtcgaa
    18241 agtgcgttac caatcagcgg gccgccataa taggctttaa ccacagcgag gctgacttta
    18301 ccctcagcga agttcaccac ctgcaatgcg ccgggatact caatcagtcg gtaaatatta
    18361 tcgataacca actgctctgg tgcgatcaga tgatcaatcg gtacagcatc tgaatgaaat
    18421 agcttatcgg catcgcgcac gtagtctggt gagcggatgc gagcgatgcg attaggggtg
    18481 ttgaaaagtg agtaggctac ctggcaggca accatattgg tttcatctga actggttaca
    18541 gcaaccagca tatcggcgtc gtcggcacct gcctcccgca atacgcgtgg atgagagcca
    18601 tgcccctgca cgactcgcag gtcaaattta tcctgcaagg tccgcagacg ctcaccgttg
    18661 gtatcgacaa cagtgatatc gttgttctcg ccaaccaggt tttccgccag tgtgccgcca
    18721 acctggccgg cacccagaat gataattttc atcagtcgcg acccgttatc tcatcacttt
    18781 ttgattagct tagcgtaaaa gaagccgtcg ccctcttcgg taccaggcag attttgttta
    18841 cccggttgct ctggtgttcc tgtttcgcaa agttcggcat cagcggtacg ttgcaaaaag
    18901 gctttaatct gcaggctatt ctcttccggt aacaccgaac aggtggcata gaccagggtt
    18961 ccaccggatt ttaaatgcgg ccaaatggcg tcgagaattt cagactgcaa ttgcgcgagt
    19021 tccgggatat cgcgatcgcg acgtaaccat ttaatatccg gatggcgacg aatcacaccg
    19081 gttgctgaac aaggcgcatc taataaaatg cgatcaaact gttgctcgcc acaccattgg
    19141 gaagggtagc ggccatcacc ttgtttcacg gtcgctttca taccaaggcg ttttaaattg
    19201 tcgtaaacgc gagagaggcg ctgttcgtca atatcaaccg caacaacctg cgcttctggt
    19261 gccacctcaa ggatatgcgt tgttttaccg ccgggggcgg cacaaagatc cagaatgtgt
    19321 tcaccgtttt gtggcgcaag ccaggtcatg caaccttgtg ctgatgcatc ctgaacggta
    19381 acccatccgt cttcaaaacc aggtagcgca tgaacaggtg caggtgtttc cagccgtaca
    19441 gcatcagggt aatccgcatg cgggaaacct ttcattcctg cttcatccaa caatgcaagc
    19501 cagctgtcgc gggaatgatg cgtacgatta acgcgcagcc acattggcgg acgctggtta
    19561 ttagcttcga cgatggattg ccactgctct ggatacgctt tttgcagacg cttcagcaac
    19621 caggaaggat gcagataacg tgcatcactg gcataaaacc cggctaataa ctcttcttgc
    19681 tgacgctgga actggcgtaa tacaccgtta atcagcccct taagttgcgg gcgcttaatt
    19741 gcgacagcgc cttcaaccgt ttcagccagc gcagcatgag gtggaatgcg ggtataaagc
    19801 agttgataca caccaaccat aatcaggtaa tgcacggtcc gctgtttgcc ggtcatcgga
    19861 cgggccatta acttattaat cagccagtct aactgtgaaa gcgtacgcag tacgccaaag
    19921 cacaactctt gaagaagtgc tttgtcttta tcggaaactt tttgctggag cggtggcaga
    19981 atgttgctta atgattgccc ttgctcgacg atttgttcaa cggcctgtgc cgccatgcta
    20041 cgtaaattac gttgtttttt cataagtata aaaatgcccg gtaagaccgg gcttagaaga
    20101 gtggactatc agaccagacg gttgcccgga acaaaccatt cccgacgaga attcaggagg
    20161 tcttgcgcgc tcatcgcttt cttacccgca ggttgtaacg agagcaggtt caggatgcca
    20221 tcaccagtcg caacctgaat gccttgttta ttggcttcaa ggatcgttcc tggtgcagcg
    20281 ttggttgccg tatcaatgac cgacgctttc cagactttaa ccggttgtcc ttcaatttcc
    20341 agccagctca ttggccatgg gttgaaagcg cgaatgcagc gttcaagctg cgctgccgaa
    20401 agcgaccagt caatacgcgc ttcttcttta ctcaacttct cggcgtaagt gacaagagtt
    20461 tcgtcctgaa cttctggttt cgccgtgccg tctgctagtt gtttcaacgt ggtgataagc
    20521 ccttgtgggc caagctctgc cagcttgtcg tacagcgtac cactggtatc ttctgcagta
    20581 atcgggcagg agagcttata aagcatgtca ccggtgtcta aaccaacatc catttgcata
    20641 atggtcacac cagtttctgc atcacccgcc catagtgagc gttggattgg tgcagcaccg
    20701 cgccagcgtg gcagcagtga accatgaacg ttgatacagc caagacgcgg catctccagc
    20761 actgctttag gcagaattaa gccataggcg acgacgacca taacatcagc ctgcagatcg
    20821 gcgaccagtt gctggctttc ttgtggacgc agggaaacag gttgaaaaac gggcagacct
    20881 ttttcctcag ccagaacttt aaccgggctg ggcatcagtt ttttaccgcg tcctgccggt
    20941 cggtctggct gggtgaacac gccaacgacg ttatgaccag aagacaacag cgcgtcgaga
    21001 tgacgcgctg caaagtcagg tgtacccgca aaaataatac gtagtgattc tgacacgtta
    21061 gttcttatcc ttaagcccgg gctttcagac gatctagttt ttcaactttc tgacgaatac
    21121 gttgttgttt cagcggtgac agataatcca taaacagttt gccgaccagg tgatccatct
    21181 catgctgaat acagatggct aacagcccct ctgcttccag ttcaaatggt ttaccgtcgc
    21241 ggtcaagggc gcgaatttta actttctctg cgcgcggcac taaagcacgt tgttcaggga
    21301 tcgacaggca accttcttca atgcctgttt cgccgctttt ttctaaaagc tccggattga
    21361 ttaacaccag ccgttcgtca cggttttccg aaacatcaat aacaatgata cgttgatgga
    21421 tatcaacctg ggttgccgcc aggccaatac cttcttctgc gtacatcgtc tcgaacatat
    21481 catcgacgat acgctgtatt tctgcattca cttcttctac cggtttagca actttgcgaa
    21541 gccgctcgtc cggaatatgt aacacttgca aaactgacat aaatctccag agatgtgttc
    21601 aggagttaga aagattattt cttctattct agacaaatcc ccctctgatt gacagcatca
    21661 ctgaccaatc gcaaagattg ctaaggctgc ttatggcagg gagataagga tggtcgatac
    21721 agaaatttgg ctgcgtttaa tgagtatcag cagcttgtac ggcgatgata tggtccgtat
    21781 agctcactgg ctggcaaaac agtcgcaaat tgatgcggtt ggattgcagc aaacagggct
    21841 tacattgcgg caggcacaac gctttctttc atttccgcga aagagtatcg aaagctcact
    21901 ttgttggttg gagcaaccca accatcattt aatccctgcg gacagcgaat tttatcctcc
    21961 tcaacttctg gcgacgacag attaccccgg cgcactgttt gttgaaggag aactgcacgc
    22021 gctgcattca tttcagcttg ccgtagtggg gagtcgggcg cattcatggt atggcgagcg
    22081 atggggacgg ttattttgcg aaactctggc gacgcgtgga gtgacaatta cgagtggact
    22141 ggcgcgtgga atcgatggtg tggcgcataa agcggcctta caggtaaatg gcgtcagcat
    22201 tgctgtattg gggaatggac ttaataccat tcatccccgc cgccatgccc gactggctgc
    22261 cagtctgctt gaacatggcg gagctctcgt ctcggaattt cccctcgatg ttccacccct
    22321 tgcttacaat ttcccacgaa gaaatcgcat tatcagtggt ctaagtaaag gtgtactggt
    22381 ggtggaagcg gctttgcgca gtggttcgct ggtgacagca cgttgtgcgc ttgagcaggg
    22441 gcgtgaagtt tttgccttgc caggtccaat agggaatccg ggaagcgaag ggcctcactg
    22501 gttaataaaa caaggtgcga ttcttgtgac ggaaccggaa gaaattctgg aaaacttgca
    22561 atttggattg cactggttgc cagacgcccc tgaaaattca ttttattcac cagatcagca
    22621 agacgtggca ttgccatttc ctgagctcct ggctaacgta ggagatgagg taacacctgt
    22681 tgacgtcgtc gctgaacgtg ccggccaacc tgtgccagag gtagttactc aactactcga
    22741 actggagtta gcaggatgga tcgcagctgt acccggcggc tatgtccgat tgaggagggc
    22801 atgccatgtt cgacgtacta atgtatttgt ttgaaaccta tattcacaca gaagctgagt
    22861 tgcgtgtgga tcaagacaaa cttgaacagg atcttaccga cgcaggattt gatcgagaag
    22921 atatctacaa tgccctgctt tggctggaaa agcttgcgga ttatcaggaa ggactggcag
    22981 aaccgatgca actggcctct gatccactct ccaatgcgta tttatacccc tgaggagtgt
    23041 gaaagactgg atgccagctg tcgtggtttt ctgcttttcc ttgagcaaat tcaggtgctt
    23101 aaccttgaaa cccgtgaaat ggtgatagag cgagtgctcg cgctggatac cgcagagttc
    23161 gacctggaag acctgaaatg ggtaatcctg atggtgttgt tcaatattcc gggctgcgaa
    23221 aatgcgtacc agcaaatgga agaattactc tttgaagtga atgaaggtat gctgcattaa
    23281 tcatttcttt aattcagcat aagttgttat ggcgaaatca gcactgttca cggtgcgtaa
    23341 taatgagtcc tgcccaaagt gcggggctga actggttatt cgatccggga aacacggtcc
    23401 gtttcttgga tgctcacagt atccggcgtg tgactacgtc cgtcctctga aatcttcagc
    23461 ggatggacat attgtcaaag ttctggaggg gcaggtttgc cttgcatgtg gcgcaaatct
    23521 ggtattacgc cagggacgct ttggtatgtt tattggttgc agtaactacc ctgaatgcga
    23581 acataccgaa cttatcgata aaccggacga aacagcaatt acatgccccc aatgccggac
    23641 gggccatctg gtccagcgcc gctcccgtta tggcaaaaca tttcactcct gtgatcgcta
    23701 cccggagtgt caatttgcca ttaacttcaa acccatagct ggagaatgcc ctgagtgtca
    23761 ttatccacta ctcatcgaaa agaaaaccgc gcagggtgta aaacactttt gtgccagtaa
    23821 acaatgtgga aagccggttt cggcggaata ataacgtgaa taataacctg caaggagacg
    23881 ctatcgcagc tgcgatagat gtcctcaatg aagaacgtgt catcgcctat ccaacggaag
    23941 ccgttttcgg tgttgggtgc gatcctgata gcgaaacagc agtgatgaga ctgttggagt
    24001 taaaacagcg tccggttgat aaggggctga ttttaatcgc ggcaaattac gagcagctta
    24061 aaccctatat tgatgacacc atgctgactg atgcgcagcg tgaaaccatt ttttcccgct
    24121 ggccaggtcc tgtcaccttt gtctttcccg cgcctgcgac aacaccgcgc tggttgacgg
    24181 gccgctttga ttcgcttgct gtacgagtca ccgaccatcc gttggtggtt gctttgtgcc
    24241 aggcttatgg taaaccgctg gtttctacca gtgccaactt gagtggattg ccaccttgtc
    24301 gaacagtaga cgaagttcgc gcacaatttg gcgcggcgtt cccggttgtg cctggtgaaa
    24361 cgggggggcg tttaaatcct tcagaaatcc gcgatgccct gacgggtgaa ctgtttcgac
    24421 aggggtaaca taatggaaac ctatgctgtt tttggtaatc cgatagccca cagcaaatcg
    24481 ccattcattc atcagcaatt tgctcagcaa ctgaatattg aacatcccta tgggcgcgtg
    24541 ttggcaccca tcaatgattt catcaacaca ctgaacgctt tctttcgtgc tggtggtaaa
    24601 ggtgcgaatg tgacggtgcc ttttaaagaa gaggcttttg ccagagcgga tgagcttact
    24661 gaacgggcag cgttggctgg tgctgttaat accctcaagc ggttagaaga tggacgcctg
    24721 ctgggtgaca ataccgatgg tataggtttg ttaagcgatc tggaacgtct gtcttttatc
    24781 cgtcctggtt tacgtattct gctcatcggc gctggtggag catctcgcgg cgtactactg
    24841 ccacttcttt ccctggactg tgcggtgaca atcactaatc ggacggtatc ccgcgcggaa
    24901 gagttggcta aattgtttgc gcacactggc agtattcagg cgttgggtat ggacgaactg
    24961 gaaggtcatg agtttgatct cattattaat gcaacatcca gtggcatcag tggtgatatt
    25021 ccggcgatcc cgtcatcgct cattcatcca ggcatttatt gctatgacat gttctatcag
    25081 aaaggaaaaa ctccttttct ggcatggtgt gagcagcgag gctcaaagcg taatgctgat
    25141 ggtttaggaa tgctggtggc acaggcggct catgcctttc ttctctggca cggtgttctg
    25201 cctgacgtag aaccagttat aaagcaattg caggaggaat tgtccgcgtg aatcaggcca
    25261 tccagtttcc ggacagagaa gagtgggacg agaataaaaa atgtgtatgt tttcccgctc
    25321 tcgtgaatgg catgcaactg acatgtgcga tctctggcga gagtctggcg tatcgtttta
    25381 ctggagatac gccagaacag tggttagcga gttttcgtca gcatcgctgg gatctggaag
    25441 aagaagcgga aaacttaatt caggaacaaa gtgaagatga tcaaggctgg gtctggttac
    25501 cctgatccag atattcgtcc ttccatttca cgtaattatt cgcggaatag cgtaacccag
    25561 ccttctcttc atcacttaac gggcggatct gtttgacggg gctaccgaga tacagatatc
    25621 cgctctccag ccgtttattt tgtgggacca gactacccgc accaatcatc acatcatctt
    25681 ctactattgc gccatcaagt aaaattgagc ccatcccaac caaaactcga ttgccaatgg
    25741 tgcagccgtg gagcatcacc ttgtgaccaa cagtgacatc ttcgccaatg gttaatgggt
    25801 tgccatctgg gttgtacgag gatttatgag tgacatgcaa catactgcca tcctggatat
    25861 tggtgcgtgc tccgatctgt acataatgta catctccacg aatcacaacg agcggccaga
    25921 tccccacatc atcagccaga cgaacgtcac caatcacgac actgctatcg tcgatcatta
    25981 cgcgctgacc gatttgtgga aaaagatcgc ggtatgggcg taaaacatca gacatactta
    26041 cctcagcaat aaatgattta ctaatgactt tgggggcatt attggccttg tgcaagtctt
    26101 ttagtatgca aaaaagcacc gttttgtgtg cgaatgcagc aaaaagggtg aaaaaacaac
    26161 aaacagaaaa aaagatcaaa aaagtacttg tgcaaaaaat tgggatccct ataatgcgcc
    26221 tccgttgaga cgacaacgtg aaacacttca caagatggtc gtaacaacga agagaaaaaa
    26281 tcctgaaatt aagggttgac tctgaaagag gaaagcgtaa tatacgccac ctcgcgacag
    26341 tgagctgaaa gccgcgtcgc aactgctctt taacaattta tcagacaatc tgtgtgggca
    26401 ctcgaagata cggattctta acgtcgcaag acgaaaaatg aataccaagt ctcaagagtg
    26461 aacacgtaat tcattacgaa gtttaattct ttgagcatca aac
//
LOCUS       DAZFWL020000058        26216 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFWL020000058 DAZFWL020000000
VERSION     DAZFWL020000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.058"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(114..2687)
                     /gene="clpB"
                     /locus_tag="ACHKNP_RS12855"
                     /old_locus_tag="pgaptmp_002571"
     CDS             complement(114..2687)
                     /gene="clpB"
                     /locus_tag="ACHKNP_RS12855"
                     /old_locus_tag="pgaptmp_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HGU4704715.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2817..3548)
                     /gene="yfiH"
                     /locus_tag="ACHKNP_RS12860"
                     /old_locus_tag="pgaptmp_002572"
     CDS             complement(2817..3548)
                     /gene="yfiH"
                     /locus_tag="ACHKNP_RS12860"
                     /old_locus_tag="pgaptmp_002572"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HGU4704716.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="ACHKNP_RS12865"
                     /old_locus_tag="pgaptmp_002573"
     CDS             complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="ACHKNP_RS12865"
                     /old_locus_tag="pgaptmp_002573"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HGU4704717.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGELVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4660..5397
                     /gene="bamD"
                     /locus_tag="ACHKNP_RS12870"
                     /old_locus_tag="pgaptmp_002574"
     CDS             4660..5397
                     /gene="bamD"
                     /locus_tag="ACHKNP_RS12870"
                     /old_locus_tag="pgaptmp_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HGU4704718.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5668..6009
                     /gene="raiA"
                     /locus_tag="ACHKNP_RS12875"
                     /old_locus_tag="pgaptmp_002575"
     CDS             5668..6009
                     /gene="raiA"
                     /locus_tag="ACHKNP_RS12875"
                     /old_locus_tag="pgaptmp_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGU4704719.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6112..6237
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6113..6160
                     /gene="pheL"
                     /locus_tag="ACHKNP_RS12880"
                     /old_locus_tag="pgaptmp_002576"
     CDS             6113..6160
                     /gene="pheL"
                     /locus_tag="ACHKNP_RS12880"
                     /old_locus_tag="pgaptmp_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HGU4704720.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6259..7419
                     /gene="pheA"
                     /locus_tag="ACHKNP_RS12885"
                     /old_locus_tag="pgaptmp_002577"
     CDS             6259..7419
                     /gene="pheA"
                     /locus_tag="ACHKNP_RS12885"
                     /old_locus_tag="pgaptmp_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HGU4704721.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="ACHKNP_RS12890"
                     /old_locus_tag="pgaptmp_002578"
     CDS             complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="ACHKNP_RS12890"
                     /old_locus_tag="pgaptmp_002578"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HGU4704722.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="ACHKNP_RS12895"
                     /old_locus_tag="pgaptmp_002579"
     CDS             complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="ACHKNP_RS12895"
                     /old_locus_tag="pgaptmp_002579"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HGU4704723.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9874..10239
                     /gene="yfiL"
                     /locus_tag="ACHKNP_RS12900"
                     /old_locus_tag="pgaptmp_002580"
     CDS             9874..10239
                     /gene="yfiL"
                     /locus_tag="ACHKNP_RS12900"
                     /old_locus_tag="pgaptmp_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HGU4704724.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10389..10907
                     /gene="yfiR"
                     /locus_tag="ACHKNP_RS12905"
                     /old_locus_tag="pgaptmp_002581"
     CDS             10389..10907
                     /gene="yfiR"
                     /locus_tag="ACHKNP_RS12905"
                     /old_locus_tag="pgaptmp_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HGU4704725.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10897..12123
                     /gene="dgcN"
                     /locus_tag="ACHKNP_RS12910"
                     /old_locus_tag="pgaptmp_002582"
     CDS             10897..12123
                     /gene="dgcN"
                     /locus_tag="ACHKNP_RS12910"
                     /old_locus_tag="pgaptmp_002582"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HGU4704726.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAKKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12139..12621
                     /gene="yfiB"
                     /locus_tag="ACHKNP_RS12915"
                     /old_locus_tag="pgaptmp_002583"
     CDS             12139..12621
                     /gene="yfiB"
                     /locus_tag="ACHKNP_RS12915"
                     /old_locus_tag="pgaptmp_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU4704727.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12698..13045)
                     /gene="rplS"
                     /locus_tag="ACHKNP_RS12920"
                     /old_locus_tag="pgaptmp_002584"
     CDS             complement(12698..13045)
                     /gene="rplS"
                     /locus_tag="ACHKNP_RS12920"
                     /old_locus_tag="pgaptmp_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGU4704728.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13087..13854)
                     /gene="trmD"
                     /locus_tag="ACHKNP_RS12925"
                     /old_locus_tag="pgaptmp_002585"
     CDS             complement(13087..13854)
                     /gene="trmD"
                     /locus_tag="ACHKNP_RS12925"
                     /old_locus_tag="pgaptmp_002585"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGU4704729.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13885..14433)
                     /gene="rimM"
                     /locus_tag="ACHKNP_RS12930"
                     /old_locus_tag="pgaptmp_002586"
     CDS             complement(13885..14433)
                     /gene="rimM"
                     /locus_tag="ACHKNP_RS12930"
                     /old_locus_tag="pgaptmp_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGU4704730.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14452..14700)
                     /gene="rpsP"
                     /locus_tag="ACHKNP_RS12935"
                     /old_locus_tag="pgaptmp_002587"
     CDS             complement(14452..14700)
                     /gene="rpsP"
                     /locus_tag="ACHKNP_RS12935"
                     /old_locus_tag="pgaptmp_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGU4704731.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14949..16310)
                     /gene="ffh"
                     /locus_tag="ACHKNP_RS12940"
                     /old_locus_tag="pgaptmp_002588"
     CDS             complement(14949..16310)
                     /gene="ffh"
                     /locus_tag="ACHKNP_RS12940"
                     /old_locus_tag="pgaptmp_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGU4704732.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTIKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16477..17268
                     /gene="ypjD"
                     /locus_tag="ACHKNP_RS12945"
                     /old_locus_tag="pgaptmp_002589"
     CDS             16477..17268
                     /gene="ypjD"
                     /locus_tag="ACHKNP_RS12945"
                     /old_locus_tag="pgaptmp_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HGU4704733.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17289..18575
                     /gene="yfjD"
                     /locus_tag="ACHKNP_RS12950"
                     /old_locus_tag="pgaptmp_002590"
     CDS             17289..18575
                     /gene="yfjD"
                     /locus_tag="ACHKNP_RS12950"
                     /old_locus_tag="pgaptmp_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HGU4704734.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18630..19223)
                     /gene="grpE"
                     /locus_tag="ACHKNP_RS12955"
                     /old_locus_tag="pgaptmp_002591"
     CDS             complement(18630..19223)
                     /gene="grpE"
                     /locus_tag="ACHKNP_RS12955"
                     /old_locus_tag="pgaptmp_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGU4704735.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19346..20224
                     /gene="nadK"
                     /locus_tag="ACHKNP_RS12960"
                     /old_locus_tag="pgaptmp_002592"
     CDS             19346..20224
                     /gene="nadK"
                     /locus_tag="ACHKNP_RS12960"
                     /old_locus_tag="pgaptmp_002592"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HGU4704736.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20310..21971
                     /gene="recN"
                     /locus_tag="ACHKNP_RS12965"
                     /old_locus_tag="pgaptmp_002593"
     CDS             20310..21971
                     /gene="recN"
                     /locus_tag="ACHKNP_RS12965"
                     /old_locus_tag="pgaptmp_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGU4704737.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22120..22461
                     /gene="bamE"
                     /locus_tag="ACHKNP_RS12970"
                     /old_locus_tag="pgaptmp_002594"
     CDS             22120..22461
                     /gene="bamE"
                     /locus_tag="ACHKNP_RS12970"
                     /old_locus_tag="pgaptmp_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HGU4704738.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22523..22813)
                     /gene="yfjF"
                     /locus_tag="ACHKNP_RS12975"
                     /old_locus_tag="pgaptmp_002595"
     CDS             complement(22523..22813)
                     /gene="yfjF"
                     /locus_tag="ACHKNP_RS12975"
                     /old_locus_tag="pgaptmp_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HGU4704739.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22803..23240)
                     /gene="ratA"
                     /locus_tag="ACHKNP_RS12980"
                     /old_locus_tag="pgaptmp_002596"
     CDS             complement(22803..23240)
                     /gene="ratA"
                     /locus_tag="ACHKNP_RS12980"
                     /old_locus_tag="pgaptmp_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HGU4704740.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23411..23893
                     /gene="smpB"
                     /locus_tag="ACHKNP_RS12985"
                     /old_locus_tag="pgaptmp_002597"
     CDS             23411..23893
                     /gene="smpB"
                     /locus_tag="ACHKNP_RS12985"
                     /old_locus_tag="pgaptmp_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGU4704741.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24108..24470
                     /gene="ssrA"
                     /locus_tag="ACHKNP_RS12990"
                     /old_locus_tag="pgaptmp_002598"
     tmRNA           24108..24470
                     /gene="ssrA"
                     /locus_tag="ACHKNP_RS12990"
                     /old_locus_tag="pgaptmp_002598"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24741..24989
                     /locus_tag="ACHKNP_RS12995"
                     /old_locus_tag="pgaptmp_002599"
                     /pseudo
     CDS             24741..24989
                     /locus_tag="ACHKNP_RS12995"
                     /old_locus_tag="pgaptmp_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
     gene            complement(25098..25255)
                     /locus_tag="ACHKNP_RS13000"
                     /old_locus_tag="pgaptmp_002600"
                     /pseudo
     CDS             complement(25098..25255)
                     /locus_tag="ACHKNP_RS13000"
                     /old_locus_tag="pgaptmp_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            25584..>26216
                     /gene="nleG7"
                     /locus_tag="ACHKNP_RS13005"
                     /old_locus_tag="pgaptmp_002601"
     CDS             25584..>26216
                     /gene="nleG7"
                     /locus_tag="ACHKNP_RS13005"
                     /old_locus_tag="pgaptmp_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HGU4704742.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGVFVKNALNSNICTL
                     YDKSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFIIMDADQQ"
BASE COUNT         6789 a         6855 c         6056 g         6516 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca tttttcaggc aaagcatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat catttactgg
      121 acggcgacaa tccggtcttc attaacttcc aggcgaatca ctttacccgg aaccaattca
      181 ccagacagta tttgctgtgc cagcgggttt tcgatctgct gctggatagc acgtttcaaa
      241 ggacgtgcac catagactgg atcgtaaccg ttctcgctca gcagtttcag cgcctcgtca
      301 gaaatgtgga tttcataacc acgttcttcc agacgtttgt acagacgttt caactgaatc
      361 tgcgcaatcg aggcaatgtg ctgttcaccc agcggatgga agaccaccac ttcatcaata
      421 cggttaatga attccggacg gaagttatgg cttaccacac cgagcaccag ctctttcatg
      481 tgcgcataat ccagttcacc gaagcgttcc tgaatcaaat cggaaccgag gttagaggtc
      541 ataatgacaa ccgtattacg gaagtcgacc gttctcccct gcccgtcagt cagacgacca
      601 tcatccagta cctgcaacag aatgttgaag acatccggat gcgctttttc cacttcatcc
      661 agcaggatga cggaatacgg acgacgacgc acagcttcgg tcaggtagcc accttcttca
      721 taaccgacat atcccggagg cgcaccaacc agacgagaca ccgagtgttt ctccataaac
      781 tcggacatat cgatacggac catcgcctcg tcgctatcaa acataaagtt cgccagcgcc
      841 ttacaaagct cagttttccc cacacccgtt gggccgagga acaggaatga accaatcggg
      901 cgatttggat ccgccaaccc cgcacggcta cggcgaatag cgttagatac cgcatcaacc
      961 gcttcgttct gaccaatgac gcgatggtgc agttcttgct ccatacgcag cagtttttcg
     1021 cgctcgcttt ccatcatgcg agaaaccgga atccccgtcc aacgcgccag cacttcagca
     1081 atttcggcgt cggtcacttt attacgcaac agacgcatag ttttgccttc gagctgcgtt
     1141 gcggcttcca gttgcttttc cagttccggg attttgccgt attgcagttc agacatccgc
     1201 gccaggtccc ccacacggcg agcctgttca atagcgattt tcgcctgttc cagttccgct
     1261 ttaatggtct gcgtaccaga aagcgatgcc ttctctgctt tccactcttc ttctaactcg
     1321 gagtactgac gttctttgtc gctcagttct tcgttgagca tatccaggcg ttttttactg
     1381 gcttcatcag actctttcat taacgcctgt tgttccagtt tgagctggat gatacgacga
     1441 tcgagtcggt cgagttcttc tggttttgag tcaatctgca tacgaatgct ggatgctgct
     1501 tcatcgatca ggtcgatggc tttatccggc agctgacggt cagcaatgta gcgatgagac
     1561 aacgtcgccg ctgcaacaat tgccgggtca gtaatttgca catggtggtg caattcgtaa
     1621 cgttctttca ggccacgcag aatcgcaatg gtatcttcaa cagaaggctc ggcaacaaac
     1681 actttctgga aacgacgttc cagcgcagca tctttttcga tgtactggcg atattcgtca
     1741 agcgtcgtgg cacctacgca gtgcaattca ccacgcgcca gcgccggttt cagcatgttt
     1801 ccggcgtcca ttgcgccatc ggctttaccc gcgccgacca tggtatgtaa ttcgtcgata
     1861 aacaggatga cgttgccttc ctgtttggca agatcgttaa gcacgccttt taaacgttct
     1921 tcaaactcac cgcgatattt cgccccagcc accagcgcgc ccatatccag cgccagtacg
     1981 cggcggcctt tcaatccttc cggcacttcg ccgttgataa tacgctgcgc cagaccttca
     2041 acgatggcag ttttaccgac gccgggttca ccaatcagta ccgggttatt tttagtacga
     2101 cgttgcagca cctgaatggt acggcgaatt tcttcatcac gaccaatcac cggatcgagt
     2161 ttgccctgtt cggctcgttc ggtaaggtcg atggtatatt ttttcaaagc ctgacgttgg
     2221 tcttcagcac cttgatcgtt cacgctttca cctccacgca tttgttcaat cgcttgagta
     2281 atgttggcgg tggtcgcccc tgctgctttc aggatgtcgg ccagcgtgcc gcgagactca
     2341 agtgccgcca gaacgaacag ttctgacgag ataaagttat caccacgttt ttgcgccagc
     2401 ttgtcgcaaa gattaagaac gcgcaccaga tcctgtgatg gctggacatc accaccagta
     2461 ccttcaacct gcggtaaacg atttaatgcc tgattgatat ctgtgcgcaa ctggccagca
     2521 tttatgccag cggatgttaa taaaggacta accgaacccc cttcctgatt cagcagggcg
     2581 ctcattaaat gaagtggttc gataaattgg ttgtcgtgcc cgagtgcaag tgattgggca
     2641 tcggcaagag caagctggaa tttattagta agacgatcca gacgcataac tcctcccata
     2701 acggatcaaa gttgctactg gagattaaat gaggtcatcc ctcaattatt caaggttatt
     2761 gaccagatta atgtgaaaag aaaatcacgc gtaccggatc gtcttgattc tttaggttat
     2821 atcagccaaa tgaaacttgc catacgaccg gtggtcttgt cgcgacgata agagaagaaa
     2881 gtctcatttt ccgtatatgt acaacggtcg ccgccgaaaa tttgctcaac acccacgttc
     2941 gccagacgct gccgggcaag ctgataaata tccgccagat acttatcacc atgctgaatg
     3001 aaagctgcac ttgctttagc gtctactgcc ataaacgcct cgcgaacctc cacccccact
     3061 tcgaacgcgc gtggaccaat tgccggccct aaccaggcga gaatattttc cggattatca
     3121 gcaaaacagg aaaccgtctc ttccagcacg cctgcgcaca gtccacgcca gccagcatga
     3181 gcggcggcga cttccgttcc cgctcgattg caaaacagca cagggaggca gtcggcagtc
     3241 atcactgcgc aaaccgtgcc gggcgtattg ctataagagg catctgctcg ttttgaggca
     3301 taaggttcgc cagtaagctt aagcacatct ttgccgtgta cctgctcaag ccagaccggt
     3361 ttagaaggca aattgcccgc agcaaaaagt cgcttgcgat tctcctcaac gtgatccggg
     3421 ttatcgccac aatgggcacc gaggttgagc gagtcatacg ggggcaagct cacgccgccg
     3481 atacgagaag agctacaggc cgcaacacct tttggtagcg gccactgcgg gacaatcagc
     3541 ttactcataa ccagtccact tcatccttat gctcttcgaa atcggcgcgc atcacctcaa
     3601 tcagctccac catatcttgt ggaataggcg catgccattc catttcgatg ccggagatcg
     3661 ggtgataaag acgcagcatg gttgcatgaa gcgcctggcg gtcaaactta cgcagcatgg
     3721 agataaatgc ttccgaagca ccttttggcg gacgcggacg gccaccataa accggatcgc
     3781 ccaccagcgg atgagtgata tgggccatat gcacgcggat ctggtgcgta cgtccagttt
     3841 ccagacgcaa ccgcagacgc gtgtgcacac ggaagtgttc catgatgcga tagtgagtca
     3901 ccgccggttt gcccatcgga tgcaccgcca tgtgagtacg tttggtcggg tggcggctga
     3961 tgggttcatc tactgtaccg ccagcggtca tatgaccaat cgccaccgct tcatactcac
     4021 gagtaatttc acgccgttgc aaagattcga ctaaacgcgt ctgagccgga acggtttttg
     4081 ccacaaccat caggccagtg gtgtctttat ccagacgatg gacgatgccc gcacgtggta
     4141 catcggcaat gggcggatag taatgaagca acgcattcag taccgtgcca tccgggttac
     4201 ccgcgccagg atgtaccacc aggtcgcgcg gtttattaat gacgatgata tcttcatctt
     4261 catagacgat atccagcggg atatcctgcg gttcaaaacg cgcttcttct tcaatctcag
     4321 cattgatggc aaccagctcg ccacccaata ctttttcttt cggcttatca caaactttgc
     4381 cgtttaccag cacgcgctgg tcgaggatcc attcttttat tcgcgaacgt gaataatccg
     4441 ggaacatttc ggccaaagcc tgatctaagc gttgaccgag ttggttttcg gacaccgttg
     4501 cagtgagctg tactcgttgt gccatatata ctgcttcttc gtttatcgtt ggggttttac
     4561 ggctttgccg tttaatatag tgtgctattg tagctggtct taaccgggag caggaacaga
     4621 gaatctcccg tattacattt tgaggaaagt caaaacgtca tgacgcgcat gaaatatctg
     4681 gtggcagccg ccacactaag cctgtttttg gcgggttgct cggggtcaaa ggaagaagta
     4741 cctgataatc cgccaaatga aatttacgcg actgcacaac aaaagctgca ggacggtaac
     4801 tggagacagg caataacgca actggaagcg ttagataatc gctatccgtt tggtccgtat
     4861 tcgcagcagg tgcagctgga tctcatctac gcctactata aaaacgccga tttgccgtta
     4921 gcgcaggctg ccatcgatcg ttttattcgc cttaacccga cccatccgaa tatcgattat
     4981 gtcatgtaca tgcgtggcct gaccaatatg gcgctggatg acagtgcgct gcaagggttc
     5041 tttggcgttg accgtagcga tcgcgatcct caacatgcac gagctgcgtt tagtgacttt
     5101 tccaaactgg tgcgcggcta tccaaacagt cagtacacca ccgatgccac caaacgtctg
     5161 gtattcctga aagatcgtct ggcgaaatat gaatactccg tggccgagta ctatacagaa
     5221 cgtggcgcat gggttgccgt cgttaaccgc gtagaaggaa tgttgcgcga ctacccggat
     5281 acccaggcta cgcgtgatgc gctgccgctg atggaaaatg cataccgtca gatgcagatg
     5341 aatgcgcaag ctgaaaaagt agcgaaaatc atcgccgcaa acagcagcaa tacataacag
     5401 aaacctgaaa tacaaaacgg cagcccttga gctgccgttt ttttattctg ttagttgtga
     5461 aactgaagcg atttagtcac tatcggtctc atcaaatatg gctcgctttg agatattcct
     5521 caagtaaaaa aacacctctt cctgcgattt ctcacaaaaa agattcgttg acaaaaagtg
     5581 acaaaattat gagattccca tcacacattt tgacatcagg agcggtatgc tgaattcacc
     5641 aagacgggaa gacaagaggt aaaatttatg acaatgaaca ttaccagcaa acaaatggaa
     5701 attactccgg ccatccgcca acatgtcgca gaccgtctcg ccaaactgga aaaatggcaa
     5761 acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt tgttgctgac
     5821 gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac
     5881 actgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa
     5941 ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa
     6001 gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg
     6061 acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca
     6121 cacaccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg tttcgtcgtg
     6181 tgaaacagaa tgcgaagacg aacaataagg cctcccaaat cggggggcct tttttattga
     6241 taacaaaaag gcaacactat gacatcggaa aacccgttac tggcgctgcg agagaaaatc
     6301 agcgcgctgg atgaaaaatt attagcatta ctggcagagc ggcgcgaatt ggccgtcgag
     6361 gtaggaaaag ccaaactgct ctcgcatcgc ccggtacgag atattgatcg tgaacgcgat
     6421 ttactggaaa gattaattac gctcggtaaa gcgcaccatc tggacgccca ttacattact
     6481 cgcctgttcc agctcatcat tgaagattcc gtattaactc agcaggcttt gctccagcaa
     6541 catctcaata aaattaatcc gcactcagca cgcatcgctt ttctcggccc caaaggttct
     6601 tattcccatc ttgcggcgcg ccagtatgct gcccgtcact ttgagcaatt cattgaaagt
     6661 ggctgcgcca aatttgccga tatttttaat caggtggaaa ccggccaggc cgactatgcc
     6721 gtcgtaccga ttgaaaatac cagctccggt gccataaacg acgtttacga tctgctgcaa
     6781 cataccagct tgtcgattgt tggcgagatg acgttaacta tcgaccattg tttgttggtc
     6841 tccggcacga ctgatttatc cactattaat acggtctaca gccatccgca gccattccag
     6901 caatgcagca aattccttaa tcgttatcct cactggaaga ttgaatatac cgaaagtacg
     6961 tctgcggcaa tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc gttgggaagc
     7021 gaagctggcg gcactttgta cggtttgcag gtactggagc gtattgaagc gaatcagcaa
     7081 caaaacttca cccgatttgt ggtgttggca cgtaaagcca ttaacgtgtc tgaccaggtt
     7141 ccggcgaaaa cgacgttgtt aatggcgacc gggcaacaag ccggtgcgct ggttgaagcg
     7201 ttgctggtgc tgcgcaacca caatctgatt atgacccgtc tggaatcacg cccgattcac
     7261 ggtaatccat gggaagagat gttttatctg gatattcagg ccaatcttga atcagcggaa
     7321 atgcaaaaag cattgaaaga gttaggggaa attacccgtt caatgaaggt attgggctgt
     7381 tacccaagtg agaacgtagt gcctgttgat ccaacctgat gaaaaggtgc cggatgatgt
     7441 gaatcatccg gcactggatt attactggcg gttgtcattc gcctgacgca ataacacgcg
     7501 gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct tgcggaaact
     7561 gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc cgaaacgctt
     7621 atagtaacgt ttgattaacg ccagattacg ctctgacgac ataataatgt cggcataaag
     7681 ctgcggatcc tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt aaatcggcga
     7741 agagagcgcc agaagttgct caagctgaac attttcttct gccagatgca gcccataagc
     7801 aaaagtagca aagtggcgca gagcctgaat aaacgccata ttctgatcgt gctcgacagc
     7861 gctaatacga tgcagccgag cgccccagac ctgaatttgc tccagaaacc attggtatgc
     7921 ttccggctta cgtccatcac accagaccac aacttgcttt gccaggctac cgctgtccgg
     7981 gccgaacatc gggtgtaacc ccagtaccgg gccatcgtgc gccgccagca tggcctgtaa
     8041 tggtccattt ttcactgatg ccagatcaac cagaatacaa tctttcggta aaggcggtaa
     8101 tttgccgata acttgctcag taacgtggat tggcacacta acaatcacca ttccggcatc
     8161 ggcaacaata tcagccgctc gatcccagtc atgttgctcc agaatccgca cctgataacc
     8221 cgatagtgtc agcatcttct cgaacaggcg tcccatctga ccgccaccgc cgacgataac
     8281 caccggacgc agtgacggac aaagtgtttt aaatcctttg tcgttttcac tggagtaaga
     8341 ttcacgcatc acccgacgca aaacatcctc aatcagatct ggcggtacac ccagagcttc
     8401 cgcctctgca cgacgcgagg ccaacataga tgcctcgcgc tccggaacat aaataggcag
     8461 tccaaagcgg cttttcacct cgcccacttc agcaaccagt tccagacgct tcgctaataa
     8521 attcagcagc gctttatcga cttcatcaat ttgatcgcgt aatgcggtca attcagcaac
     8581 cataataaac ctcttaagcc acgcgagccg tcagctgccc gttcagatcc tgatgaattt
     8641 cacgcagcaa ggcatcggtc atttcccagc taatgcaggc atcggttacg gatacaccgt
     8701 atttcatttc actgcgcggt tgctcagaag actgattgcc ctcgtggata ttactttcga
     8761 tcatcagacc aataattgag cgattgccat ctttgatttg agcaaccacg gattctgcca
     8821 ccgcaggctg acggcgataa tctttattgg aattaccgtg gctgcaatct accatcagag
     8881 acgggcgcag tcccgcctgt tccatctctt tttcacattg cgcaacatcc gcagggctat
     8941 agttcggcgc tttaccaccg cgcaggatga catgcccgtc cggattcccc tgagtttgta
     9001 gcaacgcaac ctgccctgcc tggttaatgc caacaaaacg gtgcggctgg gcggcggcgc
     9061 gcatagcgtt aattgctgtt gccagactgc cgtcggtgcc gtttttaaaa ccaaccggca
     9121 tggaaagccc ggaggccatt tcacggtgag tttgcgattc cgttgtacga gcaccaattg
     9181 ctgaccagct aaacagatcg cccaggtatt gcgggctatt cggatctaac gcttccgtcg
     9241 ccagtggcag tcccatattc accagctcaa gcagcaattt acgcgcgatc tgcagcccgg
     9301 cttctacatc aaaagagcca tccatatggg gatcgttaat taaccctttc cagccgacag
     9361 tggtacgggg tttttcaaaa tagacgcgca ttaccagata gaggctatcg ctgacctctg
     9421 cggcaagggc tttaaatcga cgagcatatt ccagagcagt ttccggatca tgaatggaac
     9481 aaggaccaca tactaccagc agacgaggat cgcgcccggc gataatatct gaaatggttc
     9541 tacgcgagtc agcaatctgg gcttcttgtt gcaggctcaa tggaaaagcg gccttcagtt
     9601 gttccggagt cattaaaacc tgttcgtcgg taatatgtac gttattcagc gcgtcttttt
     9661 gcatgatggc gatcctgttt atgctcgttt gcgatagttg atcctcagcg aggatgacgt
     9721 aacgataaca cataaagtaa agttttcaat ccatatttcg tacattttta tttacacagg
     9781 caatttagtc gcgatttcaa cccttacttc tgtatagata aatttacact ccctttgaaa
     9841 acaatccgct atgctttgaa aaaggagaaa gaaatgatga aaaagtttat cgcccccttg
     9901 ttggctttac tggttagcgg atgtcagatt gatccttata ctcacgcgcc aaccttgacc
     9961 agcaccgact ggtatgatgt tggtatggaa gatgcgatat cgggcagcgc cataaaagat
    10021 gacgatgcat ttagcgattc acaggcggat cgcggtctat accttaaagg atatgccgaa
    10081 ggacaaaaga aaacttgcca gaccgatttt acttatgccc ggggactttc cggtaaaagc
    10141 tttcctgcca gctgcaacaa tgttgaaaat gccagccaac tgcatgaagt atggcaaaaa
    10201 ggggctgatg aaaatgccag cacaatacga ttaaattaat taaaacccca caaataaatt
    10261 aaacataaga ttttactcat tgtgttttat ttctcacatt gatgacgatc gcatgaaaaa
    10321 atgatatttt aatgagcatt ctgaaaaaat atcgatgcat ttcgagcgaa gatggtgggg
    10381 atccctgaat gcgtttttct caccgactgt tcttacttct gattctcctg ctgacgggag
    10441 caccaatact cgctcaggag ccttctgacg ttgcgaaaaa cgtgcgtatg atggtttccg
    10501 gtattgtcag ttacacccgc tggcctgcgc tatcagggcc accaaagtta tgtatctttt
    10561 catcttcacg ttttagtacc gccctacaag agaatgccgc gacatctttg ccttatctgc
    10621 ctgtcatcat tcacactcaa caagaggcga tgatttcagg ctgcaatggt ttttattttg
    10681 gcaacgagtc accgacattc cagatggaat taaccgaaca atatccgtca aaggcgttat
    10741 tattaattgc cgaacaaaat accgagtgca ttattggtag cgccttttgt ctgatcatcc
    10801 acaataatga cgtcagattt gccgtaaacc tggatgcctt atcgcgtagc ggtgtaaagg
    10861 tcaacccgga tgtcttaatg ctcgcacgga agaaaaatga tggataacga taattctctt
    10921 aataagcgcc ccacgtttaa aagagcatta cgcaacatca gtatgaccag catatttatc
    10981 actatgatgc tgatctggtt gctgctttcc gtgacctcgg tgctgaccct gaaacagtac
    11041 gcgcaaaaaa acctggcact gacagcagca acaatgactt acagtctgga agcagctgtc
    11101 gtttttgccg atggccctgc agcaactgaa acactggcag cgctgggcca gcaagggcaa
    11161 ttttcaactg cagaagtacg tgataagcag caaaatattc tggcgtcctg gcattacacc
    11221 cgtaaggatc caggcgatac tttcagcaat ttcataagcc actggctctt ccccgccccc
    11281 atcattcagc cgattcgtca caatggtgaa accattggcg aagtacgctt aaccgctcgc
    11341 gacagttcaa tcagccattt tatctggttt tcgctcgccg tactgaccgg ttgtattctg
    11401 ctggcatcag gcatcgcaat taccctcacc cgccatttgc acaatggcct ggtagaagcg
    11461 ctgaaaaata tcaccgatgt cgtacatgat gtgcgttcca accgcaattt ttcccgacga
    11521 gtttcggaag aacgtatcgc tgagtttcac cgcttcgctc tcgacttcaa cagtctgctg
    11581 gatgaaatgg aagagtggca gcttcgttta caggccaaaa atgcgcagct tctgcgtacc
    11641 gcgctacatg acccattaac cgggctggct aaccgcgcag cgtttcgtag cggcatcaat
    11701 acgttgatga acaattccga tgcccgaaaa acgtcggcgt tactatttct tgatggcgat
    11761 aatttcaaat atatcaatga tacctggggt catgcgacgg gcgatagagt cttgattgaa
    11821 atcgcaaaac ggttagctga atttggcggg ctgcgacata aagcataccg cctgggcggc
    11881 gatgaattcg ctatggtgct ctatgatgta cagtcggaat ctgaagtgca gcagatatgc
    11941 tcagcactga cacaaatctt taatctcccg tttgatcttc ataatggcca tcagaccacc
    12001 atgacattaa gcattggtta cgcgatgacc attgagcacg cctctgcgaa aaaattacaa
    12061 gagcttgccg atcacaatat gtatcaggcc aaacaccagc gtgccgaaaa gctggtgaga
    12121 taacaaggat atatcgatat gataaagcac ctggtagcac ccctgatttt cacctcacta
    12181 atactgactg gctgccagtc ccctcaggga aagtttactc ctgagcaagt cgccgctatg
    12241 caatcttatg gatttactga atccgccggc gactggtcgc tgggcttatc agatgccatt
    12301 ctgttcgcaa aaaatgacta caaattgctc ccggaaagcc agcaacagat ccaaaccatg
    12361 gcagccaaat tggcctcgac agggctaaca catgcccgta tggatggaca caccgataac
    12421 tatggtgaag acagttacaa cgaaggctta tcattgaaac gggcgaatgt cgtggccgat
    12481 gcatgggcta tgggtggaca aattccgcgc agcaatctca ccacacaggg tttaggaaaa
    12541 aaatatccca tagccagtaa caagaccgcc cagggccgcg ccgagaaccg ccgcgtcgca
    12601 gtggtgatta ctacccctta aaacaccagc aaacagataa aaaaagggcc agccaattgg
    12661 ccagcccttc ttaacaggat gtcgcttaag cgaaatctta gttaagacgc tctttgatac
    12721 gagcagcctt accagtacgc tcacgcaggt agtacagttt agctttacga acagcaccac
    12781 gacgtttgac agaaatgctg tcaactaccg gagagtgagt ctggaagaca cgctcaacgc
    12841 cttcgccgtt ggaaatttta cgaacagtga atgcagagtg cagaccgcgg ttacgaatag
    12901 cgataaccac gccctcgaat gcctgcagac gttttttgga accttcaaca acccatactt
    12961 tcacttccac ggtatcaccc ggacggaagg aaggtacgtc ctgcttcatc tgctcttgtt
    13021 caagttgctt aataatgttg ctcataattt aatctcttat cctgggtaaa ctgatatctc
    13081 gggggcttac gccatcccat catgtttatg ttgctgttgt gcgtgttccg ttttgaactc
    13141 cgccagcaac cttgcttgct cttcagtcag agccaggttt tccagaagtt caggtcttct
    13201 aagccaggta cggcccagcg actgtttcaa acgccagcga cgtatctcgg catggttgcc
    13261 cgacagtaac actggcggaa cttccatccc ttctaacacc tcaggccgcg tatagtgcgg
    13321 gcaatccagc aatccttcag caaaggaatc ttccgttgcc gaggcttcat gtcccagtac
    13381 tcccggaata aaccgggaaa cggagtcaat cagcgtcatt gctggtaact caccaccact
    13441 gagaacgtaa tcgccgattg accattcttc gtcaatttcg gtttggatca cgcgctcatc
    13501 tataccttcg tagcgaccgc acaccagaat caatttttga ttcgttgcca gttcgctgac
    13561 gcccgcttga tcaagcttgc gtccctgtgg tgacagataa atcacctttg cgccttcacc
    13621 cgccgcggct tttgctgcat gaatggcgtc ccgcaagggt tgcaccatca ttaacatccc
    13681 cggtccgccg ccgtaaggac gatcgtccac ggtacggtgc cggtcatgcg tgaagtcgcg
    13741 aggactccaa ctctggatgc tcagcaggcc atttttaact gcccggccag ttaccccgta
    13801 atcggtaatt gcgcggaaca tttcaggaaa caggctaatt atgccaatcc acatagcgcc
    13861 gtcttttacc gtttatccgg tggtttaaaa accaggatcc caatctactt cgattgaacg
    13921 agtagtgaga tcgactttct tgataacctg cccatcgagg aacggtacga gacgttcctt
    13981 gataccaaac gcatctttca ggtttgcctt aatgacgaga acgtcattag atccggtttc
    14041 catcatatcg acgactttac cgagatcgta gccttcagtg gttactacct ggcagcccat
    14101 caggtctttc cagtagtagt ccccctcttc aagctgaggc agctgcgatg aatccacgac
    14161 aatttcacaa ttcgtcagca ggttcgccgc atcacgatcg tcaacgcctt tcagcttgat
    14221 gatcatgtcc tgattgtggt gcttccagct ttccagctgg acttgctgcc actgacccgc
    14281 cttctggata aaccagggct gatagtcaaa aatgctttcg gcgtcttcgg tggaagaaaa
    14341 cactctgagc cacccacgaa taccgtaaga cgaacccatt tttcccaaaa cgatgggatc
    14401 aacaggtgct tgcgcggtga gttgtttgct catcatgacc accgtgacag attaagctgc
    14461 tttgtttact tctttgatca gcgcagcaac gcgatcagaa atagttgcgc cctggccaac
    14521 ccagtgagcg atgcgatcca gatccaggcg agtgccttct tctttttcgc tagcgattgg
    14581 gttgaagaaa ccaacgcgct cgatgaagcg accgttgcgt gcattacggc tgtcagcgac
    14641 aacaacctgg tagaacggac gctttttagc gccgtgacgt gctaaacgaa tagttaccat
    14701 aacatcctct tgtgtgaata aaacaaccgg accccatcga ggaacggagt ccggtgtcat
    14761 attaaaagcc cgaaaatttt actcattttt gcgggaattg caatcaacag ttgctaactc
    14821 tgctgtaaaa ggccgtcggc ggtgcagcca gtttggtgcc ggagtgcgcg cagtcaccgg
    14881 agcgtacacg cagtacgtga ggatgacgag cacatcccgg tgccaaaatg gcaaacaagc
    14941 caggccgatt agcgaccagg gaagcctggg ggcatcatac ccttcatgct tctcatcatc
    15001 ttcgccattc cgcctttctt cattttcttc atcatgcgct gcatgtcgtc gaactgcttc
    15061 agaaggcggt tcacgtcctg cacctgcata ccgcaaccgg cagcgatacg acgcttacgc
    15121 gaacctttga tgatttctgg cttagcgcgc tcttttatcg tcatcgagtt gatgatggct
    15181 tccatacgca ccagcacttt atcgtccatc tgcgacttaa cattatccgg gatctgcccc
    15241 atgcccggca gcttgcccat caggctagcc atgccgccca tatttttcat ctggcgcagt
    15301 tgctcaagaa aatcgttgag atcgaagccg tcgccttttt tcagcttgct ggctaatttc
    15361 tctgcctgcg cgcggtcaac tttgctttcg atatcttcga tcagcgacag tacgtcgccc
    15421 atgccgagaa tacgcgacgc gatgcggtcc ggatggaacg gctccagcgc ctcagttttc
    15481 tcgccaacac cgagaaactt gatcggtttg ccagtgatgt gacgaataga gagcgccgca
    15541 ccgccgcggg catcgccgtc cactttggtc aacactacgc cggtaagcgg taacgcttca
    15601 ttgaatgctt ttgccgtatt ggccgcatcc tgaccggtca tggcgtcaac cacaaacagg
    15661 gtttcaaccg ggttaatcga cgcatggact tgtttgatct cgtccatcat cgcttcgtca
    15721 acgtgcagac gaccagcggt atccaccagc agcacgtcgt agaatttcag tttggcttct
    15781 ttcagcgccg cgttaacgat atctaccggc ttctgaccaa catcagaagg gaagaaatca
    15841 acgcccacct gctctgccag cgtctcaagc tgtttgattg ccgccgggcg ataaacgtcg
    15901 gcagaaacca ccagcacttt cttcttgtgc ttctcgcgca ggaacttacc gagtttacca
    15961 acgctggttg ttttaccggc accttgcagg cccgccatca gtacgaccgc aggcggttgc
    16021 gcagccaggt tcagggtctg gttctcttcg cccatcgccg caaccagttc gttacggact
    16081 attttgacga actcctgccc cggcgtcagg ctcttattaa cttcatgacc aaccgctttc
    16141 tcttttacgc gattgataaa ctcacgcact accggcagag cgacgtccgc ctccagcagc
    16201 gccatgcgca cttcgcgcag cgtatctttt acgttgtctt cagtgaggcg tccacggcca
    16261 ctgatattgc gcagcgtgcg cgacaaacga tcggttaaat tatcaaacat tgtctctcgc
    16321 ctggggtgga aacggttggc cgcaatcgcg acacatcatc agtattttgc cgcagtataa
    16381 catgaaggcg tctttgttgt tatgcaacgg ttggagcagc gttcacctga cgctatactg
    16441 cttctctttc ttattgctca aactgtcgac attactatgc ccgtttttgc tctgctcgcg
    16501 cttgtcgcct actccgtcag tcttgcgctg attgttcccg gtctgctgca aaaaaacggc
    16561 ggctggcggc gcatggctat tatttctgcg gtcattgcgc tggtctgcca cgcaatcgct
    16621 ctggaagccc gcatcctgcc cgacggtgat agcggacaaa acctcagcct gctgaacgtt
    16681 ggttcattgg tcagtttgat gatctgtacg gtaatgacca ttgtggcttc tcgcaatcgt
    16741 ggctggttgc tgctacccat tgtctatgcc tttgcgctta tcaacctggc gctggcgacc
    16801 ttcatgccca atgaatacat cacccatctg gaagcaacgc ctgggatgct ggtgcacatt
    16861 ggcttatcgc tcttttccta tgccacgctg ataattgccg ccctgtacgc gctacaactg
    16921 gcgtggattg attaccaact gaagaacaag aagctggcgt ttaaccaaga aatgccgcca
    16981 ttgatgagta tcgagcgtaa aatgttccac atcacgcaga ttggcgtggt gctactaacg
    17041 ctcacgcttt gcactggcct gttctacatg cacaacttat ttagcatgga aaatatcgac
    17101 aaggccgtgc tctctatcgt ggcgtggttt gtctatattg tgctgctgtg gggacattat
    17161 catgaaggat ggcgtggacg ccgcgtcgtc tggtttaacg ttgcgggtgc agtcatcctg
    17221 acactggcct acttcggcag ccgaattgtc cagcagttaa tcagctaaac ccagaaaagg
    17281 agtttcccct ggaacacatt tctactacta cgttgatcat tattctgatc atcatggtgg
    17341 tcatttcagc ctatttttcc gggtccgaaa ccggaatgat gaccctcaac cgctatcgtc
    17401 tgcgacatat ggcgaaacag ggtaatcgct cggccaaacg cgtcgaaaaa ttgctgcgta
    17461 agccagaccg cctgataagc ctggtgttaa tcggcaataa cctggtcaat attcttgcct
    17521 ccgcgctcgg cactattgtt gggatgcgtt tgtacggcga tgcgggcgtg gcaattgcga
    17581 ctggtgtgct gacttttgtc gtactggttt ttgctgaggt attgccgaaa accattgccg
    17641 cgctgtaccc ggaaaaagtc gcttatccga gtagttttct gctggctccg ctgcaaattt
    17701 tgatgatgcc gctggtctgg ttgctgaatg ctatcacccg tatgctgatg cgcatgatgg
    17761 gtatcaaaac cgatatcgtg gttagcggct ctttgagcaa agaagagttg cgcactatcg
    17821 tgcacgaatc acgctcacaa atttcccgtc gcaatcagga tatgctactg tctgtgctcg
    17881 atctggaaaa gatgaccgtt gatgacatca tggtgccgcg cagtgaaatt atcggtattg
    17941 atatcaacga tgactggaaa tcgattctgc gccaactctc ccactcacct cacgggcgca
    18001 tcgtgctcta ccgtgattcg ctggacgacg ccatcagtat gctgcgagta cgtgaagcct
    18061 ggcggttgat gtcggagaaa aaagagttca ccaaagaaac catgctgcgc gccgcggacg
    18121 agatctattt tgtgccggaa ggtacgccgc tcagcacgca gttggtaaag tttcagcgca
    18181 acaaaaagaa agtcggcctg gtcgtcaacg agtatggaga cattcagggg ctggtgacgg
    18241 ttgaagatat tctggaagag attgtcggcg atttcactac gtcgatgtcg ccaacacttg
    18301 ccgaagaggt cacgccgcaa aacgacggtt cggtgattat cgatggcacc gccaacgtgc
    18361 gggaaatcaa caaagccttt aactggcatc taccggaaga tgatgcccgc acggttaatg
    18421 gcgtcattct tgaggcactg gaagagatcc ctgtcgcagg cacccgcgtg cgtattggcg
    18481 agtacgatat agatattctc gacgtacagg acaatatgat taagcaggta aaagtttttc
    18541 ctgtgaaacc gctgcgcgag agtgtggcgg agtaacgaaa acggccctgc attcgcatgc
    18601 agggccgtga attattacga aaacagaaat tacgcttttg ctttcgctac agttaccatc
    18661 gccgcacgaa tcgtacgacc attcagcgta taacccttct gcataatgcc cagtacgtta
    18721 cctggcgcaa cgtcatcaga ttccaccatt gcgatagcct gatgcacatt cgggtccagc
    18781 ggcacgttag tttcggcgat cacttcaaca ccaaacttac gcacaacatc cagcatcgac
    18841 ttcagcgtca gctcaatgcc ttcaaccatc gcagacatat ccgggttagc tttatcagcc
    18901 acttccagcg cacgatccag gctatcaatc accggcagca attcgttgat gaatttctcc
    18961 agcgcgaatt tgtgggcttt ttcaatatcc agttcagtac gtcgacgcag gttttccatt
    19021 tcggctttta cacgcaaaat gccgtcacgt tcacgggtct gggcttcagc cagctgagct
    19081 tcgagattcg caattttttc atcgcgcgga tccacctgct cagcagaagc ttctggctca
    19141 actgcctcaa tctcttcgtg ctgatccatg ataatttctt ccggggcttg cccctcaggc
    19201 gttttctgtt ctttactact catgaatttc tccgcgtttt tttcgcattc atctcgctaa
    19261 cttcgcttat tatggggatc agtttcaggg tttcaaggga agcactcaca ttgtcatcaa
    19321 tcttcgcaac aaggacctcg gaaaaatgaa taatcatttc aagtgtattg gcattgtggg
    19381 acacccacgg caccccactg cactgacaac acatgaaatg ctctaccgct ggctgtgcac
    19441 aaaaggttac gaggtcatcg ttgagcaaca aatcgctcac gaactgcaac tgaagaatgt
    19501 gaaaactggc acgctcgcgg agattgggca actagctgat ctcgcggtag tcgttggtgg
    19561 cgacggtaat atgctgggcg cggcacgcac actcgcccgt tacgatatta aagttattgg
    19621 aatcaaccgt ggcaacctgg gtttcctgac tgaccttgac cccgataacg cccagcaaca
    19681 gttagccgat gtgctggaag gccactacat cagcgagaaa cgttttttgc tggaagcgca
    19741 agtctgtcag caagattgcc agaaacgcat cagcaccgcg ataaatgaag tggtgcttca
    19801 tccaggcaaa gtggcgcata tgattgagtt cgaagtgtat atcgacgaga tctttgcgtt
    19861 ttctcagcga tctgatggac taattatttc gacgccaaca ggctccaccg cctattccct
    19921 ctctgcaggc ggtcctattc tgaccccctc tctggatgcg attaccctgg tgcccatgtt
    19981 cccgcatacg ttgtcagcac gaccactggt cataaacagc agcagcacga tccgtctgcg
    20041 tttttcgcat cgccgtaacg acctggaaat cagttgcgac agccagatag cactgccgat
    20101 tcaggaaggt gaagatgtcc tgattcgtcg ctgtgattac catctgaatc tgattcatcc
    20161 gaaagattac agttatttca acacattaag caccaagctc ggctggtcaa aaaaattatt
    20221 ctaattttac gccagcctct ttactgtata taaaaccagt ttatactgta cacaataaca
    20281 gtaatggttt ttcatacagg aaaacgacta tgttggcaca actgaccatc agcaactttg
    20341 ctatcgttcg tgagcttgag attgattttc atagcggcat gaccgtaata actggcgaga
    20401 ccggcgcggg taaatctatt gcaatagatg ccctcggtct ttgtctcggt ggtcgcgctg
    20461 aagccgacat ggtgcgtacc ggcgctgctc gcgctgacct gtgcgcccgt ttttctctga
    20521 aagatacgcc agcggctctg cgctggctgg aagaaaatca gcttgaagac gggcatgaat
    20581 gtttgcttcg tcgagtgatc agcagcgatg gtcgctcccg tggtttcatc aacggtacag
    20641 ctgttcctct gtcacaactg cgcgaactgg gtcagttgct gattcagatc catggtcagc
    20701 acgctcatca attactcacc aaacctgagc accaaaaatt cctgcttgat ggctatgcca
    20761 atgaaacctc tctactgcag gaaatgaccg cacgttatca gttgtggcat caaagctgcc
    20821 gtgacctcgc gcatcatcaa cagttaagtc aggaacgcgc cgcccgtgcg gaactgctgc
    20881 aataccaatt aaaagaactt aacgaattta atccgcagcc cggagagttt gaacaaatcg
    20941 acgaagagta caaacgtctg gcgaacagcg gtcaattgct gaccaccagc cagaatgcat
    21001 tggcattaat ggccgacggt gaagacgcaa acctgcaaag tcagctttac acggctaaac
    21061 aactggtgag cgaattgatt ggcatggaca gcaaactgtc cggcgtactt gatatgctgg
    21121 aagaagctac catccagatt gctgaagcca gcgatgaact gcgccactac tgcgatcgtc
    21181 tggatctcga tcccaaccga ctatttgaac ttgaacagcg catctcaaaa cagatttcgc
    21241 tggcacgtaa acatcacgtc agccctgagg cattgccaca gtattaccag tcgctactgg
    21301 aagaacagca gcaactggac gatcaggccg actcacaaga aacgcttgcg ctggcggtaa
    21361 cgaaacatca tcagcaggca ctggaaatcg cgcgcgcatt acaccaacaa cgccagcaat
    21421 atgcagaaga acttgcacag ctgatcaccg acagtatgca tgcgctctca atgccgcatg
    21481 ggcagtttac gatcgatgtt aaatttgacg agcatcacct gggcgctgac ggtgccgatc
    21541 gtattgagtt tcgggtaacc accaacccag gtcagccaat gcagcctatt gccaaagtcg
    21601 catccggtgg tgaattgtcc cgcatcgcac tggcaatcca ggtcatcacg gcgcgtaaaa
    21661 tggaaacccc ggcactgatt tttgatgaag tggatgtagg gattagcggt ccaacagcgg
    21721 cagttgtcgg caaactgctg cgtcaactcg gcgaatcaac tcaggtgatg tgtgttaccc
    21781 acctgccaca agtcgcggga tgtggtcatc aacactattt tgtcagcaaa gaaaccgatg
    21841 gtgcgatgac agaaacgcat atgcaatccc tgaataaaaa agcgcggtta caagagctgg
    21901 cgcgcctgct tggtggcagt gaagtcacac gtaatacact ggcgaatgcg aaagaactgc
    21961 ttgcagcgta aacttttttc ctgcttcacg gtcagagtaa acagcaaaac gccgtaagac
    22021 cggaaagcaa aaggttttaa agtgatgaaa ggtctattat catcggcata ttacagatga
    22081 gccacgtact gctcgggccc gaaaaggaat caaatcacta tgcgctgtaa aacgctgact
    22141 gctgcagcag cagtactatt gatgttgacc gcaggctgtt ccactctgga gcgagtggtt
    22201 taccgtcctg acatcaacca ggggaactat ctgaccgcta acgacgtatc caaaatacgt
    22261 gttggcatga cgcaacaaca agttgcgtac gcattgggta caccgctgat gtccgatcca
    22321 tttggtacga atacctggtt ctatgtcttc cgccagcaac caggtcatga aggtgtaacg
    22381 cagcaaacgc tgacgctgac ctttaacagt agcggtgtgt tgaccaatat tgataacaaa
    22441 cctgcgctga gtggtaacta ataataaagt tgctctcaaa gacgttaaaa aaggtgctca
    22501 atgagcacct tttttctgtc tgttatttat tcgctgattt ttctgctcgt tgcctgcgaa
    22561 gctctttcgg atcggcaatg agagggcgat aaatctccac ccgatcgcca tcatgcacac
    22621 tatcgcttag ttttgccgga cggctgtaaa tgccgacttt gtttttagtt aaatcgatat
    22681 cggtacgcaa ttccagcaag ccactggcgc gaatagcttc ttcaaccgtc gcgccctcct
    22741 gcagcgtcac gcgctgcagg tactgcttct caggtagcgc ataagccacc tcaacggcaa
    22801 ttttacctgg cactgtaaac ctctttcgca cgaaccgtaa aagcctggac catattagac
    22861 gccagctctt taaacacgcg accaaaggcg agttcaatca acttattggt aaactcaaag
    22921 tcgagatgaa actcgatacg acacgcctcc tggctcagcg gcgtaaactt ccatccacca
    22981 atcaatttct tgaacggccc atccaccaga ttcataagaa tactttggtt actggtcaac
    23041 tggttgcggg tagtaaacgt tttgctgatc ccagccttag agacatctac cgccgcagtc
    23101 atctgcccag gagtggactc cagaatccga cttccggtac aacccggcaa aaactgagga
    23161 taagactgaa cgtcattcac taactgatac atttgctccg cgctgtaggg taccagtgcg
    23221 gtccggctaa tctgaggcat aacaatttcc atcaacaaaa atccaacaaa taatatcatt
    23281 tatcctgctg taaaaaaaac gctatcccgg cgctgggtaa catcgggttc atgctaagat
    23341 agagccttgt cccccgcagg attgatatgg ggtgttttcg atttcagatt accgatgatt
    23401 cacgacgctt atgacgaaga aaaaagcaca taaacctggt tcagcgacca tcgcgcttaa
    23461 caagcgcgcc cgtcacgaat actttatcga agaagagttc gaagcgggac ttgccctgca
    23521 aggctgggag gttaaatccc tgcgcgcagg aaaagccaat atcagcgaca gctacgtcct
    23581 tctgcgtgac ggagaggcat ttctgtttgg cgctaacatc acgccaatgg ccgtggcctc
    23641 cacgcatgtg gtgtgcgatc ctacccgtac ccgcaagtta cttctcaacc agcgcgaact
    23701 ggactcattg tacggtcgcg tcaatcgaga aggctatacc gtagtggcgc tctccctgta
    23761 ctggaaaaat gcctggtgca aagtgaaaat cggcgtcgcc aaaggtaaaa aacagcacga
    23821 taaacgttca gatatcaaag aacgcgaatg gcaggtggat aaagcacgta tcatgaaaaa
    23881 cgcccaccgt taaacctgca ctccaattat tgaccagttc ctcaccgcgc ctccctctcc
    23941 ggcggcgcga atgaacatct tattggctat cacatccgac acaaatgttg ccatcccatt
    24001 gcttaatcga ataaaaatca ggctacatgg gtgctaaatc tttaacgata acgccattga
    24061 ggctggtcat ggcgctcata aatctggtat acttaccttt acacattggg gctgattctg
    24121 gattcgacgg gatttgcgaa acccaaggtg catgccgagg ggcggttggc ctcgtaaaaa
    24181 gccgcaaaaa atagtcgcaa acgacgaaaa ctacgcttta gcagcttaat aacctgctta
    24241 gagccctctc tccctagcct ccgctcttag gacggggatc aagagaggtc aaacccaaaa
    24301 gagatcgcgt ggaagccctg cctggggttg aagcgttaaa acttaatcag gctagtttgt
    24361 tagtggcgtg tccgtccgca gctggcaagc gaatgtaaag actgactaag catgtagtac
    24421 cgaggatgta ggaatttcgg acgcgggttc aactcccgcc agctccacca cttttgatag
    24481 gactgcaatc ggacagcggc aataaaaaca gccacttacg gactctgacc agacagagcg
    24541 cagaccgaga aaagacaaaa aaatgcacgt aaaatgcacg tgcactttaa aagaacccca
    24601 gatctcacgg tctggttttt ttctgtttgt aactaatggt aacaaaaaca catcacctct
    24661 tggcgttcct tctccttgat actgtttatc catacagtta aaaataacac tgtatacaaa
    24721 cacagtatag agagattttt atgcgtattg aaatttgcat agccaaagaa aaaatcagca
    24781 aaatgccaaa aggatctgtt cctgccctac agcaagaaat gccgtgacgt gccagtaaac
    24841 gttatgacga tgtagaagtg atcatcaaat ccaccagcaa cgatggcctt tcagttactc
    24901 tcaccgccga taaagattct gcaaaaactt ttgttcagga gacgctgaaa gatacctggg
    24961 agtctgctga cgagtggttt gttcgctaat taacgagtaa aatcagtaac ggctggaaat
    25021 cattcaatac tcacactatc taaaagttca ccagccaaca gcggtaagtt ctttcatacg
    25081 acgtgccgcg gatcctatta ttctagtatg aaatccctaa ccgatttcaa attctccaga
    25141 ctgctcagat tctcttttat cccacgctgg cgctgataaa tctcgtcatt gcaatcgacc
    25201 tgcgctatcg ctgccgcagt tcttccagtt tctgcatcga taactccatc ttctgagcaa
    25261 taaacctcac ctatgcaata ttttatgtca attatttcaa ttgcattaat atcttgaatg
    25321 gattacatag agttaatgta tccagtactc cctattctct tgcatataat acatgttgca
    25381 acttacatct cagcgctatg aaaaaacacc acctctctct ttatgaaatt ctggatttac
    25441 caagcgctaa tttatcattt caatctacct ttaagcattg catttatctc cctacgaggt
    25501 catactttag gaagttaaat atgaatgata atatacctac agcgcgaaat cacaaacaat
    25561 cgacttgtat tacagaaaaa aacatgccta tctttttaaa cttcacagca ggcagtatct
    25621 tacctgagaa tgagctagca tctttacgtt atattgtgca gcaaaatcaa aatgatactg
    25681 taatcataaa agaacgttat aaaatggata tccgttatat cgaatcagtc aatggtttta
    25741 cagtaaatcc tgtatgcagt aatcatttct ccatatttat ggcgagacaa aacactattg
    25801 ctcgcaacct ggaacagcag atcaacaacg gacgaagttt tgcacaaata tctcaggatt
    25861 ttatgcttca attatcttca aatataggat ggaaaaaagg ggccgaaaac gcccttaaaa
    25921 ataaaatcca ttctcattca tttgttgtaa atcctgatga attctcttgc gacacacaat
    25981 ttcttaagtg cccaataaca ttatgcgttc cagaaaaagg ggtttttgtc aagaacgcac
    26041 tgaactccaa catatgcact ctttatgata agtctgcgtt catgaatctc acaagagaac
    26101 atctacccca ccctctcagc agggaaaaga tagtaaaaga aatgattatt gaaaggaata
    26161 tgtgttattt tgacaccata agtcagcatt tcataattat ggatgcagac caacag
//
LOCUS       DAZFWL020000059        25816 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFWL020000059 DAZFWL020000000
VERSION     DAZFWL020000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.059"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(318..1349)
                     /gene="rsmC"
                     /locus_tag="ACHKNP_RS13010"
                     /old_locus_tag="pgaptmp_002602"
     CDS             complement(318..1349)
                     /gene="rsmC"
                     /locus_tag="ACHKNP_RS13010"
                     /old_locus_tag="pgaptmp_002602"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HGU4704743.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVAKADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1452..1865
                     /gene="holD"
                     /locus_tag="ACHKNP_RS13015"
                     /old_locus_tag="pgaptmp_002603"
     CDS             1452..1865
                     /gene="holD"
                     /locus_tag="ACHKNP_RS13015"
                     /old_locus_tag="pgaptmp_002603"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HGU4704744.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTDLRANPTARAALWQQICTYEHDFFPRND"
     gene            1834..2280
                     /gene="rimI"
                     /locus_tag="ACHKNP_RS13020"
                     /old_locus_tag="pgaptmp_002604"
     CDS             1834..2280
                     /gene="rimI"
                     /locus_tag="ACHKNP_RS13020"
                     /old_locus_tag="pgaptmp_002604"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGU4704745.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2295..2972
                     /gene="yjjG"
                     /locus_tag="ACHKNP_RS13025"
                     /old_locus_tag="pgaptmp_002605"
     CDS             2295..2972
                     /gene="yjjG"
                     /locus_tag="ACHKNP_RS13025"
                     /old_locus_tag="pgaptmp_002605"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HGU4704746.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3063..4652
                     /gene="prfC"
                     /locus_tag="ACHKNP_RS13030"
                     /old_locus_tag="pgaptmp_002606"
     CDS             3063..4652
                     /gene="prfC"
                     /locus_tag="ACHKNP_RS13030"
                     /old_locus_tag="pgaptmp_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGU4704747.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5045..5650
                     /gene="osmY"
                     /locus_tag="ACHKNP_RS13035"
                     /old_locus_tag="pgaptmp_002607"
     CDS             5045..5650
                     /gene="osmY"
                     /locus_tag="ACHKNP_RS13035"
                     /old_locus_tag="pgaptmp_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HGU4704748.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5777..5938
                     /locus_tag="ACHKNP_RS13040"
                     /old_locus_tag="pgaptmp_002608"
     CDS             5777..5938
                     /locus_tag="ACHKNP_RS13040"
                     /old_locus_tag="pgaptmp_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HGU4704749.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6060..7133
                     /locus_tag="ACHKNP_RS13045"
                     /old_locus_tag="pgaptmp_002609"
     CDS             6060..7133
                     /locus_tag="ACHKNP_RS13045"
                     /old_locus_tag="pgaptmp_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HGU4704750.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7130..7912
                     /locus_tag="ACHKNP_RS13050"
                     /old_locus_tag="pgaptmp_002610"
     CDS             7130..7912
                     /locus_tag="ACHKNP_RS13050"
                     /old_locus_tag="pgaptmp_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4704751.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(8429..9292)
                     /locus_tag="ACHKNP_RS13055"
                     /old_locus_tag="pgaptmp_002611"
     CDS             complement(8429..9292)
                     /locus_tag="ACHKNP_RS13055"
                     /old_locus_tag="pgaptmp_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HGU4704752.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAVFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(9264..10814)
                     /locus_tag="ACHKNP_RS13060"
                     /old_locus_tag="pgaptmp_002612"
     CDS             complement(9264..10814)
                     /locus_tag="ACHKNP_RS13060"
                     /old_locus_tag="pgaptmp_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HGU4704753.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            11072..11851
                     /gene="deoC"
                     /locus_tag="ACHKNP_RS13065"
                     /old_locus_tag="pgaptmp_002613"
     CDS             11072..11851
                     /gene="deoC"
                     /locus_tag="ACHKNP_RS13065"
                     /old_locus_tag="pgaptmp_002613"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGU4704754.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            11978..13300
                     /gene="deoA"
                     /locus_tag="ACHKNP_RS13070"
                     /old_locus_tag="pgaptmp_002614"
     CDS             11978..13300
                     /gene="deoA"
                     /locus_tag="ACHKNP_RS13070"
                     /old_locus_tag="pgaptmp_002614"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HGU4704755.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            13352..14575
                     /gene="deoB"
                     /locus_tag="ACHKNP_RS13075"
                     /old_locus_tag="pgaptmp_002615"
     CDS             13352..14575
                     /gene="deoB"
                     /locus_tag="ACHKNP_RS13075"
                     /old_locus_tag="pgaptmp_002615"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGU4704756.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            14655..15374
                     /gene="deoD"
                     /locus_tag="ACHKNP_RS13080"
                     /old_locus_tag="pgaptmp_002616"
     CDS             14655..15374
                     /gene="deoD"
                     /locus_tag="ACHKNP_RS13080"
                     /old_locus_tag="pgaptmp_002616"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGU4704757.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(15535..15798)
                     /locus_tag="ACHKNP_RS13085"
                     /old_locus_tag="pgaptmp_002617"
     CDS             complement(15535..15798)
                     /locus_tag="ACHKNP_RS13085"
                     /old_locus_tag="pgaptmp_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4704758.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(15830..16846)
                     /gene="lplA"
                     /locus_tag="ACHKNP_RS13090"
                     /old_locus_tag="pgaptmp_002618"
     CDS             complement(15830..16846)
                     /gene="lplA"
                     /locus_tag="ACHKNP_RS13090"
                     /old_locus_tag="pgaptmp_002618"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HGU4704759.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEVITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            complement(16874..17518)
                     /locus_tag="ACHKNP_RS13095"
                     /old_locus_tag="pgaptmp_002619"
     CDS             complement(16874..17518)
                     /locus_tag="ACHKNP_RS13095"
                     /old_locus_tag="pgaptmp_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HGU4704760.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            17624..18592
                     /gene="serB"
                     /locus_tag="ACHKNP_RS13100"
                     /old_locus_tag="pgaptmp_002620"
     CDS             17624..18592
                     /gene="serB"
                     /locus_tag="ACHKNP_RS13100"
                     /old_locus_tag="pgaptmp_002620"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HGU4704761.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            18641..20023
                     /gene="radA"
                     /locus_tag="ACHKNP_RS13105"
                     /old_locus_tag="pgaptmp_002621"
     CDS             18641..20023
                     /gene="radA"
                     /locus_tag="ACHKNP_RS13105"
                     /old_locus_tag="pgaptmp_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGU4704762.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            20044..21276
                     /gene="nadR"
                     /locus_tag="ACHKNP_RS13110"
                     /old_locus_tag="pgaptmp_002622"
     CDS             20044..21276
                     /gene="nadR"
                     /locus_tag="ACHKNP_RS13110"
                     /old_locus_tag="pgaptmp_002622"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HGU4704763.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21583..23250)
                     /gene="ettA"
                     /locus_tag="ACHKNP_RS13115"
                     /old_locus_tag="pgaptmp_002623"
     CDS             complement(21583..23250)
                     /gene="ettA"
                     /locus_tag="ACHKNP_RS13115"
                     /old_locus_tag="pgaptmp_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HGU4704764.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            23461..25398
                     /gene="sltY"
                     /locus_tag="ACHKNP_RS13120"
                     /old_locus_tag="pgaptmp_002624"
     CDS             23461..25398
                     /gene="sltY"
                     /locus_tag="ACHKNP_RS13120"
                     /old_locus_tag="pgaptmp_002624"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HGU4704765.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     HIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSTTEWGRRY"
     gene            25488..25814
                     /gene="trpR"
                     /locus_tag="ACHKNP_RS13125"
                     /old_locus_tag="pgaptmp_002625"
     CDS             25488..25814
                     /gene="trpR"
                     /locus_tag="ACHKNP_RS13125"
                     /old_locus_tag="pgaptmp_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HGU4704766.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
BASE COUNT         5976 a         6729 c         7140 g         5971 t
ORIGIN      
        1 aattccgcca ccttcgcata ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat
       61 tctagtggtt ttcagctttt cgtcaatagt taattatcgc cggaggtgta attgctggaa
      121 aaatgtccat caggaaacta acgtgcaggt tttgtaggtc ggataaggcg ttcacgccgc
      181 atccgacacg gtatttgggt gcagatgcca gatgcgacgc gggcgcgtct tatctggcct
      241 acgaagggct aacgtgcagg ttttgtaggt tgtataaggc gagcacgccg catccgacaa
      301 ggcttgcggc gggatactta acctttcttc gcctggcggg tcataatggc gcgatacacc
      361 ttgaagcgcc cggtttgcgc gataacttca tggaagccaa atgtctcatc cagcacgtcc
      421 gggtaaggca ggaaggcgtt cgctacaatt cgcagctcgc cgccgctatt aagatgacgc
      481 accgcgccgc gaatcagcgt ttgcgccgca tccaggctgg tttgcatccc atcgtggaac
      541 ggcgggttgg agatgatcat atcaaaacga cctttcacct cggaaaagac gttgctggca
      601 aagacttcac cttcaacacc gttggccgca agcgtcgcac ggctggcttc taccgccggg
      661 gcagagacat cgcacaaggt gagacgaatt ttcggcgaat ggcgcgcaaa ggcaactgaa
      721 agcacacccg cgccacagcc gacatccagc actttacctt tcgtgtgcgg ggttaacgtc
      781 gagagcagca actggctacc gacatccaga ccgtcgcggc taaacacgcc aggcagcgtt
      841 ttgaccgtca ggccatcgac gctgtattcg ccccagaatt tatccgcatc aaatactggc
      901 tgtttttcca gacgaccaaa atagaggcca cagcgacgag cgctgtcgac tttattcaac
      961 ggcgcataat ctgccagcat ctgctcggcg ctgcgcacgc cgctgcggtt ctcgccaacg
     1021 acaaaaatat ccgtccccac cggcagcaga gaaagtaaat tcatcaactg gaactgggct
     1081 tccggtttgt tcttcggcca gtagtaaatc agcgtatcgc aatctgcgac gtcatccgcc
     1141 ttggcgacca gactaaaacg ggcgttatcc cccatctggc ggcttaatac ctgccagtgg
     1201 tggaattgct gggtatgagc acggctggcc gcggtatcta aacgcgcggg caggtcatcc
     1261 tgtaagtctc cggcaaacag aatacggctt tgttcgaaat catcactgtg acgcagcaag
     1321 acttcacttg ccggggtaaa tgcagacatg gaatgctcct caattgatac tggcggcgat
     1381 tatagccata tgttggcgcg gtatcgacga atttgctata tttgcgcccc tgacaacagg
     1441 agcgattcgc tatgacatcc cgacgagact ggcagttaca gcaactgggc attacccagt
     1501 ggtcgctgcg tcgccctggc gcgttgcagg gggaaattgc cattgcgatc ccggcacacg
     1561 tccgtctggt gatggtggca aacgatcttc ccgccctgac tgatccttta gtgagcgatg
     1621 ttctgcgcgc attaaccgtc agccccgatc aagtgctgca actgacgcca gaaaaaatcg
     1681 cgatgctgcc gcaaggcagt cgctgcaaca gctggcggtt gggtactgac gaaccgctat
     1741 cactggaagg cgctcaggtg gcatcaccgg cgctcaccga tttacgggca aacccaacgg
     1801 cacgcgccgc gttatggcaa caaatttgca catatgaaca cgatttcttc cctcgaaacg
     1861 actgatttac cagcggctta ccacattgaa caacgcgccc acgcctttcc gtggagtgaa
     1921 aaaacctttg ccagcaacca gggcgagcgt tatctcaact ttcagttaac gcaaaacggc
     1981 aaaatggcgg cgtttgcgat tacgcaagtg gtgctggatg aagctacatt gttcaatatt
     2041 gcggtcgatc ctgactatca gcgtcaggga ttgggaaggg cgctgctgga acatctgatc
     2101 gacgaactgg aaaaacgcgg cgtggcgacg ctctggctgg aagtccgtgc ctcaaatgct
     2161 gccgccattg ccctgtacga aagtttaggc tttaacgagg cgacgattcg ccgcaattac
     2221 taccccacca cggacggtcg cgaagacgcc atcatcatgg cgttgccaat cagtatgtaa
     2281 gacaaggtgg aataatgaag tgggactgga ttttctttga tgccgatgaa acgctgttta
     2341 cctttgactc attcaccggc ctgcagcgga tgtttcttga ttacagcgtc acctttaccg
     2401 ctgaagattt tcaggactat caggccgtta acaagccact gtgggtggat tatcaaaacg
     2461 gcgcgatcac ttcattacag cttcagcacg ggcgtttcga gagctgggcc gaacggctga
     2521 aagttgaagc aggcttgctt aacgatgcct ttattaatgc gatggcggaa atctgcacgc
     2581 cgctgccggg cgcggtttct ctgcttaacg ccattcgtgg caacgccaaa atcggcatca
     2641 tcaccaacgg ctttagcgcc ttgcagcaag tgcgtctgga acgcacgggc ctgcgtgatt
     2701 acttcgattt gctggtgatt tccgaagaag ttggcgttgc taaaccgaat aagaaaattt
     2761 tcgattatgc gctggaacag gcgggcaatc ctgaccgttc acgcgtgctg atggttggcg
     2821 acactgccga gtccgatatt ctcggtggca tcaacgccgg gcttgcgacc tgctggctga
     2881 atgcgcacaa tcgcgagcaa ccagaaggca tcgcgcccac ctggaccgtt tcttcgttgc
     2941 acgaactgga gcagctcctg tgtaaacact gattgcctcc cccccgttga tgggtaaaat
     3001 agccgcaatt tttcgttttc aacaagcgcg gcgcgatgcc gcttactcaa gaagaaagaa
     3061 ttatgacgtt gtctccttat ttgcaagagg tggctaagcg ccgcactttt gccattattt
     3121 ctcacccgga cgccggtaag actaccatca ccgagaaggt gctgctgttc ggacaggcca
     3181 ttcagaccgc cggtacagta aaaggccgtg gttccaacca gcacgctaag tcggactgga
     3241 tggagatgga aaagcagcgt gggatctcca ttactacgtc tgtgatgcag tttccgtatc
     3301 acgattgcct ggttaacctg ctagacaccc cggggcacga agacttctcg gaagatacct
     3361 atcgtaccct gacggcggtg gactgctgcc tgatggttat cgacgccgca aaaggtgttg
     3421 aagatcgtac ccgtaagctg atggaagtta cccgtctgcg cgacacgccg atcctcacct
     3481 ttatgaacaa acttgaccgt gatatccgcg acccgatgga actgctcgat gaagttgaga
     3541 acgagctgaa aatcggctgc gcaccgatca cctggccgat tggctgcggc aagctgttta
     3601 aaggcgttta ccacctttat aaagacgaaa cctatctcta tcagagcggt aaaggtcaca
     3661 ccattcagga agtccgcatt gttaaagggc tgaataaccc ggatctcgat gctgcggttg
     3721 gtgaagatct ggcgcagcag ctgcgtgacg aactggaact ggtgaaaggc gcgtctaacg
     3781 agttcgacaa agaattgttc cttgcgggcg aaatcactcc ggtattcttc ggtactgcgc
     3841 tgggtaactt cggtgtcgat catatgctgg atggcctggt ggagtgggct cctgcgccga
     3901 tgccgcgtca gactgatacc cgtaccgtag aagcgagcga agacaaattt accggcttcg
     3961 tatttaaaat tcaggccaac atggacccga aacaccgcga ccgcgtggcg tttatgcgcg
     4021 tggtatccgg taaatatgaa aaaggcatga agctgcgcca ggtgcgcacc gcgaaagacg
     4081 tggtgatctc tgacgcgctg acctttatgg cgggtgaccg ttcgcacgtt gaagaagcgt
     4141 atcctggcga tatcctcggc ctgcacaacc acggcaccat tcagatcggc gacaccttta
     4201 cccagggtga gatgatgaag ttcaccggta ttccgaactt cgcaccagaa ctgttccgtc
     4261 gtatccgcct gaaagatccg ctgaagcaaa aacagctgct caaagggctg gtacagcttt
     4321 ccgaagaggg cgcggtgcag gtgttccgtc cgatctccaa caacgatctg atcgttggtg
     4381 cagttggtgt gctgcagttt gatgtagtgg tatcgcgcct gaagagcgaa tacaacgttg
     4441 aagcagtata tgaatcagtc aacgttgcca ccgcccgctg ggtagaatgt gcagacgcga
     4501 agaaattcga agagttcaag cgtaagaacg aaagccaact ggcgcttgat ggcggcgata
     4561 acctcgctta catcgctacc agcatggtca acctgcgcct ggcgcaggaa cgttatccgg
     4621 acgttcagtt ccaccagacc cgcgagcatt aattcttgcc tccagggcgc ggtagccgct
     4681 gcgccctgtc aatttccctt ccttattagc cgcttacgga atgttcttaa aacattcact
     4741 tttgcttatg ttttcgctga tatcccgagc ggtttcaaaa ttgtgatcta tatttaacaa
     4801 agtaatgaca tttctgacgg cgttaaatac cgttcaatgc gtagatatca gtatctaaag
     4861 ccgtcgattg tcattctacc gatattaata actgattcag aggctgtaat ggtcgttatt
     4921 catcacccat cgcttttgtg atggcgacca ttgacttctg tagagggtga agtctctccc
     4981 tattcagcaa tgcaacctcg tgttgccagg ctcaaattac gagcaaacat acaggaataa
     5041 atcgatgact atgacaagac tgaagatttc gaaaactctg ctggctgtaa tgttgacctc
     5101 tgccgtcgcg accggctctg cctacgcgga aaacaacgca cagactacca atgaaagcgc
     5161 agggcaaaaa gtcgatagct ctatgaataa agtcggtaat ttcatggatg acagcgccat
     5221 caccgcgaaa gtgaaggcgg cgctggtgga tcatgacaac atcaagagca ccgatatctc
     5281 tgtcaaaacc gatcaaaaag tcgtgaccct gagcggtttc gttgaaagcc aggctcaggc
     5341 cgaagaggca gtgaaagtgg cgaaaggcgt tgaaggggtg acctctgtca gcgacaaact
     5401 gcacgttcgc gacgctaaag aaggctctgt gaagggctac gcgggtgaca ccgccaccac
     5461 cagtgaaatc aaagccaaac tgctggcgga cgatatcgtc ccttcccgtc atgtgaaagt
     5521 tgaaaccacc gacggcgtgg ttcagctctc cggtaccgtc gattcccagg cacagagcga
     5581 ccgtgctgaa agtatcgcca aagcggtaga tggtgtgaaa agcgttaaaa atgatctgaa
     5641 aactaagtaa ttcgtcgtaa ttcgtcctcc cgaactttgt cgggaggcgc gatgtgcacc
     5701 acactaaaaa tatcgcattg agtgattttc acgctcatat caagcggtga cattaactat
     5761 ggtaaaggag acgcttatgt ttcgttgggg catcatattt ctggttatcg cgttaatcgc
     5821 cgccgcactt gggtttggtg gtctggccgg taccgctgca ggcgcagcta aaattgtctt
     5881 tgtcgtcggg attattctgt tcctggtgag tttgttcatg ggccgaaaac gaccctagat
     5941 ttcagaccat taagaaaatt ttatccaaag ccagtccagc ggactggctt ttgcggtttt
     6001 agcgaataat aaattgcgtt actttgtcat tctttcacaa caaaaacagg aaggcagagg
     6061 tggggcagcg aatacctgta acgcttggta atattgcgcc gttgtcgcta aggccgttcc
     6121 agcctggacg aatagctctg gtgtgcgaag gcggcggaca gcgtggaatt ttcacggctg
     6181 gcgtgctgga tgagtttatg cgcgcgcagt ttaatccttt cgatctttat ctcggcacat
     6241 ctgccggggc gcagaacctc tcggcgttta tctgcaatca gcccggttac gcgcgcaaag
     6301 tcatcatgcg ctataccaca aaacgcgaat ttttcgatcc attgcgcttt gtccgtggag
     6361 gaaatcttat cgatctcgac tggctggtgg aggccaccgc aagccagatg ccgttgcaaa
     6421 tggataccgc cgcgcggttg tttgacagcg gcaaatcgtt ttatatgtgc gcctgtcgtc
     6481 aggatgacta cgcgcctaat tactttttac caaccaaaca aaactggctg gatgtgattc
     6541 gcgcctccag tgcgatacct ggcttttatc gtagcggagt gtcgctggaa ggcattaact
     6601 acctggatgg cgggatcagt gatgcgattc cggttaaaga ggcggcaagg cagggcgcta
     6661 aaacgttggt cgtcatccgc actgtgccgt cacaaatgta ctacacgccg cagtggttca
     6721 aacgcatgga acgctggctg ggtgacagta gcctgcagcc gctggtcaat ctggtgcagc
     6781 atcatgaaac cagctatcgt gacattcagc aatttattga gaaaccaccg ggcaagctgc
     6841 ggatattcga aatttatcca ccgaaaccat tacatagtat cgcgcttggc agtcggattc
     6901 cggcgctgcg tgaagactat aaacttgggc gtttatgcgg tcgttatttc ctcgccacgg
     6961 ttggcaagct attaactgaa aaagcgccgc ttacccgcca tctggtgcca gtggtgacgc
     7021 cggaatcgat cgttattccg cctgcgccag tcgccaacga tacgctggtt gccgaagtga
     7081 gcgatgctcc gcaggcgaac gacccgacat ttaacaatga ggatctggct tgatttgccg
     7141 ttttatcgat acccactgcc attttgattt cccgccgttt agtggcgatg aagaggccag
     7201 cctgcaacgc gcggcacaag cgggcgtagg caagatcatt gttccggcga cagaggcgga
     7261 aaattttgcc cgcgtgcagg cgttagcgga aaaatatcaa ccgctgtatg ccgcattggg
     7321 gttgcatccg gggatgttgg aaaaacatag cgatgtgtct cttgatcagc tacagcaggc
     7381 gctggaaagg cgtccggcga aggtggtggc ggtgggggag atcggtctgg atctcttcgg
     7441 cgacgatccg caatttgaga ggcagcagtg gttactcgac gaacagctga aactggcgaa
     7501 acgctacgat ctgccggtga tccttcattc ccggcgtaca cacgacaaac tggcgatgca
     7561 tcttaaacgt cacgatttac cgtgcactgg cgtggttcac ggtttttccg gcagcctgca
     7621 acaggccgag cggtttgtac agctgggcta caaaatcggc gtaggcggta ctatcaccta
     7681 tccacgcgcc agtaaaaccc gcgatgtcat cgcaaaatta ccgctggcat cgttgttgct
     7741 ggaaaccgac gcgccggata tgccgctcaa cggctttcag gggcagccta accgaccgga
     7801 acaggctgtg cgggtgtttg acgtgctttg cgagttgcgc ccggaaccag aagatgagat
     7861 tgccgaagtg ttgcttaata atacgtatgc gttgtttagc gttagtgggt agtttaccct
     7921 tcgtaggcag catccggcag tcggcgcata atgcctgatg cgacgcttgt cgcgtcttat
     7981 catgcctaca ggtttgtgcc gaaccgtagg ccggataagg cgttcacgcc gcatccggca
     8041 gttggcgcac aatgcctgat gcgacgcttg tcgcgtctta tcatgcctac aggtttgtgc
     8101 cgaaccgtag gccggataag gcgttcacgc cgcatccggc agtcggcgca taatgcctga
     8161 tgcgacgctt gtcgcgtctt atcatgccta caggtttgtg ccgaaccgta ggccggataa
     8221 ggcgttcacg ccgcatccgg cagttggcgc acaatgcctg atgcgacgct tgacgcgtct
     8281 tatcatgcct acaagtctgt gccgaaccgt aggccggata aggcgttcac gccgcatccg
     8341 gcagtcggcg cataatgcct gatgcgacgc ttgtcgcgtc ttatcatgcc tacaagtctg
     8401 tgccgaaccg taggccgtat ccggcatgtc acaaatagag cgccggaaat atcaaccggc
     8461 tcaccccgcg cacctttaac gcatcagcca acggctcaac gtcttccggc gtggcgctcg
     8521 cccagctttg cgcctcgcca tacacgccgt gggcatgaaa cgcgttcagg cgtaccggaa
     8581 catcgccaag tcccttgata aacaccgcca gttcttcgat gtgttgcaaa taatccacct
     8641 ggccagggat taccagcaaa cgcagttccg ccagcttacc gcgctctgcc agcaaacaga
     8701 tgctgcgctt aatctgctga ttatcgcgtc cggttaattg ttgatgacat tcgctccccc
     8761 acgctttgag atcgagcatt gcgccgtcac acaccgggag taatttttcc cagccggttt
     8821 cgctcaacat accgttactg tccacgaggc aggtgaggtg gcgcagttgc ggatcgtttt
     8881 tgatggctgt aaacagcgcc accacaaacg gcagctgggt cgtggcttcg ccgccgctta
     8941 ccgttatccc ttcgataaac agcaccgctt tgcggatatg gctaagcacc tcatccacgc
     9001 tcatggattg cgccattggc gtggcatgtt gcggacacat cttcaggcag gtatcacact
     9061 gctcgcagac cacagcgttc cacagcactt tgccgtcaac aatctgcaac gcctgatgcg
     9121 gacactgtgg cacgcactct ccgcaatcat tgcagcgtcc catcgtccac ggattgtgac
     9181 agtttttgca gcgcagattg cagccctgca aaaacagagc cagacgactg cctggcccgt
     9241 caacgcagga gaaggggata atcttactga ctaaagcgca tctgctgttc atggcttatc
     9301 acgcgcggct ggcgttccag aatacgagtg ttgcgtgcgg cttcttcgcc cagccaggtg
     9361 gtgttggtgc gtgaaccttc ggcgcgatat ttttctaaat ccgacaaacg caccatataa
     9421 ccggtaacgc gaaccagatc gttaccgctg acattggcgg taaattcacg cattccggct
     9481 ttaaaggcac cgaggcaaag ctgtaccagt gcctgcgggt tacgtttgat ggtttcgtcg
     9541 agggtcagaa tgtcgctgat gccggaataa taataagcat gatgcggggc gacagtttgc
     9601 agatgggtga tcggatctgg ctcatcgcca tacggcaaac gcgcgcccgg cgtggtgccg
     9661 atatcggaac tgatccccga ctgtgcgtgt aacatggcgc gtttttgcca gccatatttc
     9721 acgggggtat tggcgacaaa ctccgccagt tgcgcgctga tgcgataacc cacttcattt
     9781 gcggcggctt ctttaccgta gcgcgcggca atcccttctt tctcacacag caagttaacc
     9841 gcttccgcca gcccatacat gccaaacatt ggcacaaaac gttcagggtt aatcagccct
     9901 tctttcacca ggaagctatt ctcaaagaag tgtgattgtt gatagaggaa ttcacaccgc
     9961 gcatcgatga tggcgatctg ctgctggcag tagtgcggta gagtgcgcgt aaagaagtca
    10021 tccagcgatt cgctgcgctc ggcaatggct ttcaggttaa ggcgtaccag cgtgctgcca
    10081 ccacccgcca gcggcagtga gttgtaacag ctcacaatcc cgtagccccc ttttgtgaaa
    10141 attttatcat gcaccggacc gttggcgatg tgcggtttgc tacattcaca gatgttcttc
    10201 gccacttcca gcagcaggtc atcaggggtg atttctggat cgtagataaa ggtcaggttc
    10261 ggtgaaacct gcttcaactc tgcatctgca cgtaatatcg cacgggtgat gggcgaatca
    10321 gacgggccga tattggcgtg cataaaggcg tctggcaggg ttctgtcgag gtaacgccag
    10381 aaacgtttta ttcgaatatc gatctcgtct tgtgttagaa ttctaacata cggttgcaac
    10441 aacgcatcca gttgccccag gtagaccggc atcgatgtga ccgacggtac gtggtggtaa
    10501 agaatggtca gcagagagag tgcgtcatca agatctttcg cgccttccag ctccagccat
    10561 tcggaaccgt tcgccagaaa acgggcgtaa tcgggtaaga catagcgcgg tttgtacggc
    10621 gcatgacctt caaacatatc gcagattaca ccctcatcca gcgcgcggcg ggcctcggca
    10681 ggcagctgcg gataaggcag attgttttct gcttccagtg ccagaaaatg gcgcttctgc
    10741 tccgggctaa gcactgggct ggtgacaatt tgctggcaac gttgttgcag tgcattttca
    10801 tgagaagtgg gcatcttctt ttccttttat gccgaaggtg atgcgccatt gtaagaagtt
    10861 tcgtgatgtt cactttgatc ctgatgcgtt tgccaccact gacgcattca tttgaaagtg
    10921 aattatttga accagatcgc attacagtga tgcaaacttg taagtagatt tccttaattg
    10981 tgatgtatat cgaagtgtgt tgcggagtag atgttagaat actaacaaac tcgcaaggtg
    11041 aattttattg gcgacaagcc aggagaatga aatgactgat ctgaaagcaa gcagcctgcg
    11101 tgcactgaaa ttaatggacc tgaccaccct gaatgacgac gacaccgacg agaaagtaat
    11161 tgctctgtgt catcaggcca aaaccccggt cggcaatacc gccgctatct gtatctatcc
    11221 tcgctttatc ccgattgctc gcaaaacgct gaaagagcag ggcaccccgg aaatccgaat
    11281 cgctacggta accaacttcc cacacggtaa cgatgacatc gaaatcgcgc tggcagaaac
    11341 ccgtgcggca atcgcctacg gtgcagatga agttgacgtt gtgttcccgt accgcgcgct
    11401 gatggcgggc aacgagcagg ttggttttga cctggtgaaa gcctgtaaag aggcttgcgc
    11461 ggcagcgaat gtactgctga aagtgatcat cgaaaccggc gaactgaaag acgaagcgct
    11521 gatccgtaaa gcgtctgaaa tctccatcaa agcgggtgcg gacttcatca aaacctctac
    11581 cggtaaagtg gctgtgaacg cgacgccgga aagcgcgcgc atcatgatgg aagtgatccg
    11641 tgatatgggc gtagaaaaaa ccgttggttt caaaccggcg ggcggcgtgc gtactgcgga
    11701 agatgcgcag aaatatctcg ccatcgcaga tgaactgttt ggtgctgact gggctgatgc
    11761 gcgtcactac cgctttggtg cttccagcct gctggcaagc ctgctgaaag cgctgggtca
    11821 cggcgacggt aagagcgcca gcagctacta agtaagatgc tttacgcctg atgcgctgcg
    11881 cttatcaggc ctacgagacg tatctacccg taggccggat aaggcgtaga cgcatccggc
    11941 aaaagccgcc tcatactctt ttcctcggga ggttaccttg tttctcgcac aagaaattat
    12001 tcgtaaaaaa cgtgatggtc atgcgctgag cgatgaagaa attcgtttct ttatcaacgg
    12061 tattcgcgac aacactatct ccgaagggca gattgccgcc ctcgcgatga ccattttctt
    12121 ccacgatatg acaatgcctg agcgtgtctc gctgaccatg gcgatgcgag attcaggaac
    12181 cgttctcgac tggaaaagcc tgcatctgaa tggcccgatt gttgataaac actcgaccgg
    12241 cggcgtcggc gatgtgactt cgctgatgtt ggggccgatg gtcgcagcct gcggcggcta
    12301 tattccgatg atctctggtc gcggcctcgg tcatactggc ggtacgctcg acaaactgga
    12361 atccatccct ggcttcgaca ttttcccgga tgacaaccgt ttccgcgaaa ttattaaaga
    12421 cgtcggcgtg gcgattatcg gtcagaccag ctcactggct ccggcggata aacgtttcta
    12481 cgcgacccgt gatattaccg caaccgtgga ctctatcccg ctgatcactg cctcgatcct
    12541 ggcgaagaaa ctggcggaag gtctggatgc gctggtgatg gacgtgaaag tgggtagcgg
    12601 cgcgtttatg ccgacctacg aactctctga agcccttgcc gaagcgattg ttggcgtggc
    12661 taacggcgct ggcgtgcgca ccaccgcgct gctcaccgac atgaatcagg tactggcctc
    12721 cagtgcaggt aacgcggttg aagttcgtga agcggtgcag ttcctgacgg gtgagtatcg
    12781 taacccgcgt ctgtttgatg tcacgatggc gctgtgcgtg gagatgctga tctccggcaa
    12841 actggcgaaa gatgacgccg aagcgcgcgc gaaattgcag gcggtgctgg acaacggtaa
    12901 agcggcagaa gtctttggtc gtatggtagc ggcacaaaaa ggcccgaccg acttcgttga
    12961 gaactacgcg aagtatctgc cgacagcgat gctgacgaaa gcagtctatg ctgataccga
    13021 aggttttgtc agtgaaatgg atacccgcgc gctggggatg gcagtggttg caatgggcgg
    13081 tggacgccgt caggcatctg acaccatcga ttacagcgtc ggctttactg atatggcgcg
    13141 tctgggcgac caggtagacg gtcagcgtcc gctggcggtt atccacgcga aagacgaaaa
    13201 cagctggcag gaagcggcga aagcggtgaa agcggcaatt aaacttgccg ataaagcacc
    13261 ggaaagcaca ccaactgtct atcgtcgtat cagtgaataa cggtatactg atctgatcat
    13321 ttaaatttga agcactgagt acggagaaca tatgaaacgt gcatttatta tggtgctgga
    13381 ctcattcggc atcggcgcta cagaagatgc agaacgcttt ggtgacgtcg gggctgacac
    13441 cctgggtcat atcgcagaag cctgtgccaa aggcgaagct gataacggtc gtaaaggccc
    13501 gctcaatctg ccaaatctga cccgtctggg gctggcgaaa gcacacgaag gttctaccgg
    13561 tttcattccg gcgggaatgg acggcaacgc tgaagttatc ggcgcgtacg catgggcgca
    13621 cgaaatgtca tccggtaaag ataccccgtc tggtcactgg gaaatcgccg gtgtcccggt
    13681 tctgtttgag tggggatatt tctctgatca cgaaaacagc ttcccgcaag agctgctgga
    13741 taaactggtc gaacgcgcta atctgccggg ttacctcggt aactgccact cttccggtac
    13801 ggtcattctg gatcagctgg gcgaagagca catgaaaacc ggcaagccga ttttctatac
    13861 ctccgctgac tctgtgttcc agattgcctg ccacgaagaa acctttggcc tggataaact
    13921 ctacgaactg tgcgaaatcg cccgtgaaga gctgaccaac ggcggctaca acatcggtcg
    13981 tgttatcgct cgtccgttta tcggtgacaa agccggtaac ttccagcgta ccggtaaccg
    14041 tcacgacctg gctgttgagc cgccagcacc gaccgtgctg cagaaactgg ttgatgaaaa
    14101 acacggtcag gtggtttctg tcggtaaaat tgccgacatc tacgccaact gcggtatcac
    14161 caagaaagtg aaagcaactg gcctggacgc gctgtttgac gccaccatca aagagatgaa
    14221 agaagcgggt gataacacta tcgtcttcac caacttcgtt gacttcgact cttcttgggg
    14281 ccaccgtcgc gacgtcgccg gttatgctgc gggtctggag ctgttcgacc gccgtctgcc
    14341 ggagctgatg tctctgctgc gcgatgacga catcctgatc ctcactgctg accacggttg
    14401 tgatccgacc tggaccggta ctgaccacac gcgtgaacac attccggtac tggtttacgg
    14461 cccgaaagta aaaccgggct cactgggtca ccgtgaaacc ttcgcggata tcggccagac
    14521 tctggcaaaa tattttggta cttctgatat ggaatatggc aaagccatgt tctgataatg
    14581 ggtgcggctt gatgccctca ccccggccct ctcccacagg gagagggtga aaacatgtag
    14641 ataaggataa aacaatggct accccacaca ttaatgcaga aatgggcgat ttcgctgacg
    14701 tagttttgat gccaggcgac ccgctgcgtg caaagtatat tgctgaaact ttccttgaag
    14761 atgcccgtga agtgaacaac gttcgcggta tgctgggctt caccggtact tacaaaggcc
    14821 gcaaaatttc cgtaatgggt cacggtatgg gtatcccgtc ctgctccatc tacaccaaag
    14881 aactgatcac cgatttcggc gtgaagaaaa ttatccgcgt gggttcctgt ggcgcagttc
    14941 tgccgcacgt aaaactgcgc gacgtcgtta tcggtatggg tgcctgcacc gattccaaag
    15001 ttaaccgcat ccgttttaaa gatcatgact ttgccgctat cgctgacttt gacatggtgc
    15061 gtaacgcggt agacgcggct aaagcactgg gcgttgatgc tcgcgtgggt aacctgttct
    15121 ccgctgacct gttctactct ccggacggcg aaatgttcga cgtgatggaa aaatacggca
    15181 tcctcggcgt ggaaatggaa gcggctggta tctacggcgt ggctgcagag tttggcgcga
    15241 aagccctgac catctgcacc gtgtctgacc acatccgcac tcacgagcag accactgccg
    15301 ctgagcgtca gaccaccttc aacgacatga tcaaaatcgc actggaatcc gttctgctgg
    15361 gcgataaaga gtaattgtgt ttcgctgaaa ggcgaacgtc gtgttaagcc ggagcaggag
    15421 actgctccgg ctttttagtt tctattcatc aatagcaact gatttataga tttactatta
    15481 tttttcatat ggatagaaaa taaaatccat tcaagtatct attcacgaat ctattcattt
    15541 atgttcagcc gtcatccagg aagggcatat cagcaactct ggtggacaac cataaatttc
    15601 tgccattttg cttttgatgt ttgagctaag ccgttccgtc ttttcgatct gttttagaga
    15661 attaatcgag atacctaaca ttttacttac ttcagcaagc gataatttat gcctgatacg
    15721 cacggcggct agccaggaaa tacgctggct taacattaaa ttgataatct cccgctcctg
    15781 gttttccaga tcgatcatat tcccttcctt tggtatttta atcagcgagt taccttaccg
    15841 cccccgctat ccacgtcgac aactcccgta gctctttttc ctggtccggg aagtcaacca
    15901 acagcgcttc gcactcttgt tgcagcatat ccgcgcggta caggcagcct tgcagtcgcc
    15961 cggcgagggc ttccagcggc gcggggttga ggctgtcggt aaacacctgg gcgcgggtga
    16021 tatggccttt ttcaacgtcg aaatgcagtt ccacgccgcc ccagctaaag cgttcatcca
    16081 gcagatgcga gaatgccgga gcctgaccga agttccattc ccagctactc tgacgggcaa
    16141 aggtttcggc gaagtttggc aagtctggcg ttttgtccgg ggagatgatt tccgcttcta
    16201 cacgctcgcc ataatgggcg aaaaaggcct tggttatgac ctcgcaaacc tgctcatggg
    16261 tgatccccgg cagcagctcg gtgaggttgg tcacgcggga acgcactgag gtaatgcctt
    16321 tcgccgccag tttcttttta tccggattga gatagtttgc caggcggcta aggtcggcat
    16381 tgagcagcaa agtgccgtgg tggaagccac gatctttggt ttcgcgatag gccgagcctg
    16441 agactttgcg gtcgccttcg actgttttca ccaccagatc gttacgcccg gacgcttcgg
    16501 cgctgacgcc gagcgcgttc agcgcattga gcacaatcga cgtagagatg gttttatcgt
    16561 actccggctt gccagccata aaggtaaagc aggtattgcc gagatcgtgg aacaccgcgc
    16621 cgccaccgct actgcgccgc gccaggcgga cgttatcttc ttccatccgc cgggtattac
    16681 actctttcca cgggttctgc gcgcgaccaa tcactaccgt gtcggcattg cgccagagaa
    16741 acagaacgcg ctgcgtggcg ggcatttggc gaaaaataca ctcttccacc gccaggttaa
    16801 accacgggtc gtaagagtca gagatgagca ggcgtaatgt ggacataacg atttcctttc
    16861 ttgtagtaat gggtcactct tttttctcgc tttcttcctc ttccggcacc ggtttgctgg
    16921 cggttaacag gaagggcgat tgctgccagc gggtgcgttt accctgtagc agcgtgcggg
    16981 tcagcactac gccgattgcc agtgagagca gcaacatcag gcgtaaaatg ttagtggtgt
    17041 tatccacctg ttgggcttcg gtggcgagcg tatgggtgtc gagtgtcagg cgcagatagc
    17101 cgagcggtcc gtttttaccc gcaattggct cgacaatctg ctggttaaaa tagccgcctg
    17161 cttttttacc gtcgagcgcc agccggtcgc gcacttcgac gctttcgcct gaacgggcga
    17221 taagatcgcc ttgttcgtca tacacacccg cgtcgaggat gcggctttca tccgttaact
    17281 gatcgaggat cgcctgaatg cgtttttcat ctggtgagtc ggtacgcatc agcggtgcaa
    17341 cgttcagcgt cacctgacgc gccagggtgc gggccagttc ttccagctgt ggattacgct
    17401 gtcgctggtg gttttgacta aaccatgacg ccccctgcat cagtgccact aacaaggcaa
    17461 gacagaacag gacaatcact gcccgatgca gccggaattt cagttttgtg cgagccatct
    17521 tccacccttt gaaaatttga gacttaatgt tgccagaagc aatggataca aggtagcctc
    17581 atgcgttatt ttccctgctt cgaacgattt tacaggagcc ttaatgccta acattacctg
    17641 gtgcgacctg cctgaagatg tctctttatg gccgggtctg cctctttcat taagtggtga
    17701 tgaagtgatg ccactggatt accacgcagg tcgtagcggc tggctgctgt atggtcgtgg
    17761 gctggataaa caacgcctga cccaatacca gagcaaactg ggcgcggcga tggtgattgt
    17821 tgccgcctgg tgcgtggaag attatcaggt gattcgtctg gcaggttcac tcaccgcgcg
    17881 cgctacgcgc ctggcccatg aagcgcaact ggatgtcgcg ccgctgggga aaatcccgca
    17941 cctgcgcacg ccgggtttgc tggtgatgga catggattcc accgccatcc agattgaatg
    18001 tattgatgaa attgccaaac tggccggaac gggcgagatg gtggcggagg taaccgaacg
    18061 ggcgatgcgc ggcgaactcg attttaccgc cagcctgcgc agccgcgtgg cgacgctgaa
    18121 aggcgctgat gccaatattc tgcaacaggt gcgtgaaaat ctgccgctga tgccaggctt
    18181 aacgcagctg gtgctcaagc tggaaacgct gggctggaaa gtggcgattg cctccggcgg
    18241 ctttactttc tttgctgaat acctgcgcga caagctgcgc ctgaccgccg tggtagccaa
    18301 tgaactggag atcatggacg gtaaatttac cggcaatgtg atcggcgaca tcgttgacgc
    18361 gcagtacaaa gcgaaaactc tgacccgcct cgcgcaggag tatgaaatcc cgctggcgca
    18421 gaccgtggcg attggcgatg gagccaatga cctgccgatg atcaaagcgg cagggctggg
    18481 gattgcctac catgccaagc caaaagtgaa tgaaaagacg gaagtcacca tccgtcacgc
    18541 tgacctgatg ggggtattct gcatcctctc tggcagcctg aatcagaagt aattgcctgc
    18601 ccgtcatcct gcgggcggca cagcattaac gaggtacacc gtggcaaaag ctccaaaacg
    18661 cgcctttgtt tgtaatgaat gcggggccga ttatccgcgc tggcaggggc agtgcagtgc
    18721 ctgtcatgcc tggaacacca tcaccgaggt gcgtcttgct gcgtcgccaa cggtggcgcg
    18781 taacgagcgt ctcagtggct atgccggtag cgccggggtg gcaaaagtcc agaaactctc
    18841 cgacatcagc cttgaagagc tgccgcgttt ttccaccgga tttaaagagt tcgaccgcgt
    18901 attaggcggc ggcgtggtac cgggaagtgc cattctgatt ggcggtaacc caggcgcggg
    18961 gaaatccacg ctgttgctgc aaacgctgtg caaactggcc cagcagatga aaacgctgta
    19021 tgtcaccggc gaagagtcgc tgcaacaggt ggcaatgcgc gctcatcgcc ttggcctgcc
    19081 gactgacaat ctcaatatgt tgtcggaaac cagcatcgag cagatctgcc tgattgctga
    19141 agaagagcaa ccgaagctga tggtaattga ctccatccag gtgatgcata tggcggatgt
    19201 acagtcatcg cctggtagcg tggcgcaggt gcgtgaaacg gcggcttatt tgacacgctt
    19261 cgccaaaacg cgcggtgtgg cgattgtcat ggttggacac gtaaccaaag atggctcgct
    19321 ggcgggccct aaagtgctgg aacactgtat cgactgttcg gtgcttttgg atggcgatgc
    19381 cgactcccgt tttcgcacct tgcgcagcca taaaaaccgc ttcggcgcgg tgaatgagct
    19441 gggcgtcttc gcgatgaccg aacaggggct gcgtgaagtc agcaaccctt cggcaatttt
    19501 cttaagtcgc ggagatgaag tgacctccgg cagctccgtg atggtggtgt gggaaggaac
    19561 gcgtccgttg ctggtggaga ttcaggcgct ggtcgatcac tcgatgatgg cgaatccgcg
    19621 ccgcgtggcg gtaggtctgg agcaaaaccg tctggcaatc ctgctggctg tcttgcaccg
    19681 tcacggtggt ctgcaaatgg ccgatcagga tgtatttgtg aacgtggtcg gtggtgtgaa
    19741 ggtaaccgaa accagtgccg acttagcgtt actgctggcg atggtttcca gcctgcgtga
    19801 cagaccgcta ccgcaggatc tggtggtgtt tggtgaagtc gggctggcag gggagatccg
    19861 cccggtgccc agcggtcagg agcgaatctc tgaagcggcg aaacacggtt ttcgccgggc
    19921 gattgttccg gcggctaacg tgccgaaaaa agcgccggaa gggatgcaga tttttggcgt
    19981 taaaaaactc tccgacgcgc ttagcgtgtt cgacgactta taatgagaga tacggaggga
    20041 gatatgtcgt catttgatta cctgaaaact gccatcaagc aacagggctg cacgttacag
    20101 caggtagctg atgccagcgg tatgaccaaa gggtatttaa gccagttact gaatgccaaa
    20161 attaaaagcc ccagcgcgca aaagctggag gcgttgcacc gttttttggg gcttgaattt
    20221 ccccggcaga agaaaaccat tggtgtcgta ttcggtaagt tctacccgct gcataccgga
    20281 catatctacc ttatccagcg cgcctgtagc caggttgacg agctgcatat cattatgggt
    20341 tttgacgata cccgcgaccg cgcgttgttt gaagacagcg ccatgtcgca gcagcccacg
    20401 gtgccggatc gtctgcgctg gttattgcaa acttttaaat atcagaaaaa tattcgcatt
    20461 catgctttca acgaagaggg catggagccg tatccgcatg gctgggatgt gtggagcaac
    20521 ggcatcaaaa agtttatggc tgaaaagggg atccagccgg acctgatcta cacctcggaa
    20581 gaagccgatg cgccacagta tatggaacat ctggggatcg agacggtact ggtcgatccg
    20641 aaacgtacct ttatgagtat cagcggtgcg cagatccgcg aaaacccgtt ccgctactgg
    20701 gaatatattc ctacggaagt gaagccgttc tttgtgcgta ccgtggcgat ccttggcggc
    20761 gagtcgagcg gtaaatccac cctggtaaac aaacttgcca atatcttcaa taccaccagt
    20821 gcgtgggaat atggtcgcga ttatgtcttt tcacacctcg gcggtgatga gatcgcattg
    20881 cagtattccg actacgataa aatcgcgctg ggccacgcac aatacattga ttttgcagtg
    20941 aaatacgcca ataaagtggc gtttattgac actgattttg tcactaccca ggcgttctgc
    21001 aaaaagtacg aagggcgtga gcatccgttc gtgcaggcgt tgattgatga ataccgtttc
    21061 gatctggtga tcctgctgga gaacaacacg ccgtgggtgg cggatggttt acgcagcctc
    21121 ggcagttcgg tggatcgcaa agagttccag aacttgctgg tggagatgct ggaagagaac
    21181 aatatcgaat tcgtgcgggt tgaagaggac gattatgaca gccgtttcct gcgctgcgtg
    21241 gagctggtgc gggagatgat gggggagcag agataaccgc gatgaaacgg ctcaaaggcg
    21301 aggtataaaa taagtttttt gatgagttga aaacctttct ggaagaggct gtcgagatta
    21361 aacaaggttt gaaaaaacct gcacgggtga cccgctacga aattgcggat actaaggctg
    21421 ttgtaggccg gaaaaggcgt tcacgccgta tccggcattc ggtgctcaat gcctgatgcg
    21481 acgcttaccg cgtcttatca ggcctggaga ttcacaacgt attgaaattt cttggtttgt
    21541 aggccggata aggcgttcac gccgcatccg gcattttacg cattacttcg caatacgctt
    21601 gtacttgata cgcttcggct ccagtgcgtc tgcgcccagc gtgcgtttct tgtactcttc
    21661 gtactcagta aagttacctt cgaagaactc aactttacct tcatcctggt agtccaggat
    21721 gtgcgtggcg atacggtcga ggaaccaacg gtcgtgcgag ataaccatcg cacagcccgg
    21781 gaactccagc agggcgtttt ccagcgcgcg cagggtttcg atatccaggt cgttggttgg
    21841 ttcgtcgagc agcagcatgt tgccgccaac ctgcagcagc ttcgccagat gcagacgacc
    21901 gcgctcacca ccggaaagtt caccaacgcg tttaccctga tcaacccctt taaagttaaa
    21961 gcggccaacg taggcgcggc ttggcatctc ggtgttgccg atcttcataa tatccagccc
    22021 gccggaaact tcttcccaaa cggttttgct gttatccatt gagtcacgga actgatcaac
    22081 cgatgccagt ttcaccgttt cacctaaagt gatggtgccg ctgtccggct gttcctgacc
    22141 agagatcata cggaacaggg tcgatttacc cgcgccgttc ggaccgatga tcccgacgat
    22201 tgcccctttc gggatcgaga agctcaggtc atcaatcagc aggcgatcac cgtaggattt
    22261 acgcaggttg ctgacttcca gcactttatc gcccagacgc ggtccaggtg gaataaacag
    22321 ttcgttggtt tcgttacgtt tctgatattc ggtgctgttc agctcttcaa agcgtgccag
    22381 acgtgcttta cctttcgact ggcggccttt agttccctga cgtacccact ccagctcttt
    22441 ctcgatcgac ttacgacgcg ccgcttcttg tgaagcttcc tgcgccaggc gctgatcttt
    22501 ctgctccagc caggaggagt agttgccttc ccacggaata ccttcaccgc ggtcaagttc
    22561 gaggatccag cctgcaacgt tatcgaggaa gtaacggtcg tgggtaatcg ccaccacggt
    22621 gccctcgaag tcgtgcagga agcgttccag ccaggccacg gattcggcat ccaggtggtt
    22681 ggtcggttcg tcgagcagca gcatgtctgg tttttccagc agcaggcggc acaacgctac
    22741 gcgacgacgc tcaccaccgg agaggttagc gattttcgcg tcccagtccg gcagacgtag
    22801 cgcatccgcc gcacgctcca gctgtacgtt caggttatga ccgtcgtgag cctgaatgat
    22861 ctcttccaga cggccttgtt cagcggccag cttgtcaaaa tcggcatccg gatcggcgta
    22921 cagcgcatac acttcatcca ggcgtttcag ggcgttaacc acttcagaaa ccgcttcttc
    22981 aatggactca cgcacggtgt gttccgggtt cagctgcggt tcctgcggca ggtaaccaat
    23041 cttgatgtct ggctgcggac gcgcttcacc ttcgatgtct ttatcaatgc ccgccataat
    23101 gcgcagcagg gtagacttac ccgcgccgtt cagacccagg acaccaattt ttgccccagg
    23161 gaagaaactc agagagatgt ttttcaaaat atgacgtttc ggcggaacaa ctttgccgac
    23221 acgatgcatg gtataaacga attgagccac gttggacttc gcctctatgt ttatcgtgat
    23281 gatgagtttt caaaggcgaa gtgtagcctt tttccccgcc taatcccagt tggtcgatca
    23341 ccttcgcagt aaaagtaaaa aagtgtccgt aacgtggcgt aaacggcaat gactggttag
    23401 cataaatcta ttacgcggca tgacgctgca ttgatgtatt tacacttaga ggatgcgctt
    23461 gtggaaaaag ccaaacaagt tacctggcgg ctgttggctg ccggtgtctg tctgctgacg
    23521 gtcagcagcg tggcgcgagc cgactcactg gatgagcagc gtagccgtta cgcgcaaatc
    23581 aagcaggcct gggataatcg acaaatggat gtggtcgaac aaatgatgcc tggactgaag
    23641 gattatccgc tttatcccta cctggaatac cgccagatca ccgatgatct gatgaatcaa
    23701 ccggcggtga cggtcactaa ctttgttcgc gctaacccca cgcttcctcc cgctcgcacg
    23761 ctgcaatctc gtttcgtcaa tgaactggcg cggcgtgaag actggcgtgg cttgttagcc
    23821 tttagcccgg aaaagcccgg aactaccgaa gcgcaatgta attactacta tgcgaaatgg
    23881 aacaccgggc agagtgaaga agcctggcaa ggggcgaaag agctgtggct aaccggcaag
    23941 agccagccta acgcctgtga caagttattt agcgtctggc gtgcgtcagg taaacaagat
    24001 ccgctggctt atttagagcg tatccgtctg gcgatgaaag cgggtaacac tggcctggta
    24061 acagtgctgg cagggcagat gcctgccgat taccagacta tcgcctcggc aatcatttca
    24121 ctggcgaaca accctaatac ggtactgacc ttcgcgcgta caaccggagc gaccgatttt
    24181 acccgtcaaa tggcggcggt ggcgtttgcc agtgtggcgc ggcaggatgc tgaaaatgca
    24241 cggctgatga tcccgtcgct tgcccaggcg cagcagctta atgaagatca tattcaggag
    24301 ctgcgcgata tcgtcgcctg gcgtttgatg ggcaacgatg tcaccgacga gcaggcgaaa
    24361 tggcgcgatg acgccattat gcgctcgcaa tccacgtcgt tgattgaacg ccgtgtgcga
    24421 atggcacttg gcaccggcga tcgtcgcggc ctgaatacct ggctggcgcg tctgccgatg
    24481 gaagcaaaag agaaagatga atggcgttac tggcaggcgg atttattgct ggaacgcgga
    24541 cgtgaagctg aagcgaaaga gattttgcat caactcatgc aacagcgtgg tttctacccg
    24601 atggttgcag cacaacgcat cggagaagag tatgagctga agattgataa agcgccgcag
    24661 aatgttgaca gcgccctgac tcaggggccg gagatggcgc gcgtgcgcga gttgatgtac
    24721 tggaatctcg ataacaccgc gcgtagcgag tgggccaatc tggtgaagag caagtcaaaa
    24781 acagagcagg ctcaactggc tcggtatgct ttcaataacc aatggtggga tcttagcgtt
    24841 caggcaacga tcgccgggaa gctttgggat catctggaag agcgattccc gctggcttac
    24901 aacgatcttt tcaaacgtta caccagtggg aaagagatcc cgcaaagcta tgcgatggcg
    24961 attgcccgtc aggagagcgc ctggaatccg aaagtgaaat caccggtagg ggccagcggc
    25021 ctgatgcaga ttatgcctgg tacagcgacc catacggtga agatgttctc tattcctggt
    25081 tatagcagcc ctgggcaatt gctggatccg gaaacgaata tcaacattgg caccagttac
    25141 ctgcaatatg tttatcagca gtttggcaat aaccgtattt tctcctcagc agcttataac
    25201 gccggacctg gacgggtgcg aacctggctt ggcaacagcg ccgggcgtat cgacgctgtg
    25261 gcatttgtcg agagtattcc gttctccgag acgcgcggtt atgtgaagaa cgtgctggct
    25321 tatgacgctt actaccgcta tttcatgggg gataaaccga cgttgatgag caccacggaa
    25381 tggggacgtc gttactgatc cgcacgttta tgatatgcta tcgtactctt tagcgagtac
    25441 aaccggggga ggcattttgc ttcccccgct aacaatggcg acatattatg gcccaacaat
    25501 caccctattc agcagcgatg gcagaacagc gtcaccagga gtggttacgt tttgtcgacc
    25561 tgcttaagaa tgcctaccaa aacgatctcc atttaccgtt gttaaacctg atgctgacgc
    25621 cagatgagcg cgaagcgttg gggactcgcg tgcgtattgt cgaagagctg ttgcgcggcg
    25681 aaatgagcca gcgtgagtta aaaaatgaac tcggcgcggg catcgcgacg attacgcgtg
    25741 gatctaacag cctgaaagcc gcgcccgttg agctgcgcca gtggctggaa gaggtgttgc
    25801 tgaaaagcga ttgatt
//
LOCUS       DAZFWL020000060        25502 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFWL020000060 DAZFWL020000000
VERSION     DAZFWL020000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.060"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            270..689
                     /locus_tag="ACHKNP_RS13130"
                     /old_locus_tag="pgaptmp_002626"
     CDS             270..689
                     /locus_tag="ACHKNP_RS13130"
                     /old_locus_tag="pgaptmp_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HGU4704767.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            689..1957
                     /gene="umuC"
                     /locus_tag="ACHKNP_RS13135"
                     /old_locus_tag="pgaptmp_002627"
     CDS             689..1957
                     /gene="umuC"
                     /locus_tag="ACHKNP_RS13135"
                     /old_locus_tag="pgaptmp_002627"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HGU4704768.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(2003..2533)
                     /gene="dsbB"
                     /locus_tag="ACHKNP_RS13140"
                     /old_locus_tag="pgaptmp_002628"
     CDS             complement(2003..2533)
                     /gene="dsbB"
                     /locus_tag="ACHKNP_RS13140"
                     /old_locus_tag="pgaptmp_002628"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HGU4704769.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2679..4220)
                     /gene="nhaB"
                     /locus_tag="ACHKNP_RS13145"
                     /old_locus_tag="pgaptmp_002629"
     CDS             complement(2679..4220)
                     /gene="nhaB"
                     /locus_tag="ACHKNP_RS13145"
                     /old_locus_tag="pgaptmp_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HGU4704770.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4442..5161
                     /gene="fadR"
                     /locus_tag="ACHKNP_RS13150"
                     /old_locus_tag="pgaptmp_002630"
     CDS             4442..5161
                     /gene="fadR"
                     /locus_tag="ACHKNP_RS13150"
                     /old_locus_tag="pgaptmp_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HGU4704771.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5213..6745)
                     /locus_tag="ACHKNP_RS13155"
                     /old_locus_tag="pgaptmp_002631"
     CDS             complement(5213..6745)
                     /locus_tag="ACHKNP_RS13155"
                     /old_locus_tag="pgaptmp_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HGU4704772.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7075..8373
                     /gene="dadA"
                     /locus_tag="ACHKNP_RS13160"
                     /old_locus_tag="pgaptmp_002632"
     CDS             7075..8373
                     /gene="dadA"
                     /locus_tag="ACHKNP_RS13160"
                     /old_locus_tag="pgaptmp_002632"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HGU4704773.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVHPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8383..9453
                     /gene="dadX"
                     /locus_tag="ACHKNP_RS13165"
                     /old_locus_tag="pgaptmp_002633"
     CDS             8383..9453
                     /gene="dadX"
                     /locus_tag="ACHKNP_RS13165"
                     /old_locus_tag="pgaptmp_002633"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HGU4704774.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9839..11575)
                     /locus_tag="ACHKNP_RS13170"
                     /old_locus_tag="pgaptmp_002634"
     CDS             complement(9839..11575)
                     /locus_tag="ACHKNP_RS13170"
                     /old_locus_tag="pgaptmp_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HGU4704775.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11670..12584)
                     /gene="ldcA"
                     /locus_tag="ACHKNP_RS13175"
                     /old_locus_tag="pgaptmp_002635"
     CDS             complement(11670..12584)
                     /gene="ldcA"
                     /locus_tag="ACHKNP_RS13175"
                     /old_locus_tag="pgaptmp_002635"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HGU4704776.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12684..13295
                     /gene="emtA"
                     /locus_tag="ACHKNP_RS13180"
                     /old_locus_tag="pgaptmp_002636"
     CDS             12684..13295
                     /gene="emtA"
                     /locus_tag="ACHKNP_RS13180"
                     /old_locus_tag="pgaptmp_002636"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HGU4704777.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13297..14031)
                     /locus_tag="ACHKNP_RS13185"
                     /old_locus_tag="pgaptmp_002637"
     CDS             complement(13297..14031)
                     /locus_tag="ACHKNP_RS13185"
                     /old_locus_tag="pgaptmp_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HGU4704778.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14232..14486
                     /locus_tag="ACHKNP_RS13190"
                     /old_locus_tag="pgaptmp_002638"
     CDS             14232..14486
                     /locus_tag="ACHKNP_RS13190"
                     /old_locus_tag="pgaptmp_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU4704779.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            14664..15104
                     /gene="ycgY"
                     /locus_tag="ACHKNP_RS13195"
                     /old_locus_tag="pgaptmp_002639"
     CDS             14664..15104
                     /gene="ycgY"
                     /locus_tag="ACHKNP_RS13195"
                     /old_locus_tag="pgaptmp_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HGU4704780.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLAPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15183..16880)
                     /gene="treA"
                     /locus_tag="ACHKNP_RS13200"
                     /old_locus_tag="pgaptmp_002640"
     CDS             complement(15183..16880)
                     /gene="treA"
                     /locus_tag="ACHKNP_RS13200"
                     /old_locus_tag="pgaptmp_002640"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU4704781.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17200..18618)
                     /gene="dhaM"
                     /locus_tag="ACHKNP_RS13205"
                     /old_locus_tag="pgaptmp_002641"
     CDS             complement(17200..18618)
                     /gene="dhaM"
                     /locus_tag="ACHKNP_RS13205"
                     /old_locus_tag="pgaptmp_002641"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HGU4704782.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18629..19261)
                     /gene="dhaL"
                     /locus_tag="ACHKNP_RS13210"
                     /old_locus_tag="pgaptmp_002642"
     CDS             complement(18629..19261)
                     /gene="dhaL"
                     /locus_tag="ACHKNP_RS13210"
                     /old_locus_tag="pgaptmp_002642"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HGU4704783.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19272..20342)
                     /gene="dhaK"
                     /locus_tag="ACHKNP_RS13215"
                     /old_locus_tag="pgaptmp_002643"
     CDS             complement(19272..20342)
                     /gene="dhaK"
                     /locus_tag="ACHKNP_RS13215"
                     /old_locus_tag="pgaptmp_002643"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HGU4704784.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20570..22489
                     /gene="dhaR"
                     /locus_tag="ACHKNP_RS13220"
                     /old_locus_tag="pgaptmp_002644"
     CDS             20570..22489
                     /gene="dhaR"
                     /locus_tag="ACHKNP_RS13220"
                     /old_locus_tag="pgaptmp_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HGU4704785.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22589..25474)
                     /locus_tag="ACHKNP_RS13225"
                     /old_locus_tag="pgaptmp_002645"
     CDS             complement(22589..25474)
                     /locus_tag="ACHKNP_RS13225"
                     /old_locus_tag="pgaptmp_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4704786.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINEGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
BASE COUNT         6295 a         6342 c         6582 g         6283 t
ORIGIN      
        1 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
       61 gaatgcttct attctatttt ttagccgggt aatatttttc atttctgctg gatgagcgta
      121 gtcgccagaa ggccgcgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
      181 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac tactgtatat
      241 aaaaacagta tagcttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
      301 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
      361 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
      421 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
      481 tactgattgt cgatagcgct attaccgcca gccatggtga tattgtcatc gctgctgttg
      541 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
      601 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
      661 tgatccacgt cgttaaggcg atgcgctgat gtttgctctc tgtgatgtaa acgcgtttta
      721 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
      781 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gtgttaaaat
      841 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
      901 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
      961 atcgccccgc gtcgagattt acagtattga tgaggcattc tgcgatctga caggtgtgcg
     1021 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     1081 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ctggctaagc ttgccaatca
     1141 tgcggcaaaa aaatggcagc ggcagacggg gggggtggtg gatttatcaa atctggaacg
     1201 ccagcgtaaa ttaatgtctg ctctccccgt ggatgaagtc tgggggattg gacggcggat
     1261 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctt gatttggcgg atacagatat
     1321 ccggtttatc cgtaaacatt ttaatgtcgt gctcgaaaga acggtgcgtg aactgcgcgg
     1381 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     1441 ctcgtttggt gaacgcatca cggattatcc atcgatgcgg caggccattt gtagttacgc
     1501 tgcccgggcg gcggaaaaac ttcgcagcga gcatcaatat tgtcggttta tctccacatt
     1561 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     1621 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     1681 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     1741 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     1801 gcaattgatg acggtaatgg atacactgaa tgctaaagag ggcagaggaa cactctattt
     1861 tgccgggcag gggatccagc aacaatggca gatgaagcgt gccatgcttt caccacgtta
     1921 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     1981 tcccgcagga gcgccgaatg gattagcgac cgaacaggtc acgttttttc gctttaaacg
     2041 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     2101 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     2161 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     2221 cacaggtggc aaacggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     2281 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     2341 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     2401 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     2461 gtgccagagc agtaaacgcc atcaacaacc acgcgccccg gccttgtgaa cattggttca
     2521 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     2581 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa ttggacaatt atcgggcgaa
     2641 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     2701 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     2761 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     2821 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     2881 gcgacggagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     2941 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     3001 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     3061 gcatgttccg atgcctgcaa cacaaactga ataattggcg aaaacagttg ttggtcgata
     3121 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     3181 aaggctttac cgatagcatg ttcatcggtg acaccggtca atgatgtcgc cagaataatg
     3241 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     3301 acgccaataa tcgcctggac aatcagacgg attttatcct gacgagtacg ctggtggcgg
     3361 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     3421 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     3481 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     3541 atgatcaggt tctgtggttc gcccaccatg gtcatcacgc cgcctaatgc agtgccgaca
     3601 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cactttgtaa
     3661 tgcttgtcga tatgactatc gtcttgcagg tcggtatctt cagtacggga agaggctacg
     3721 cggtgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     3781 gcatcgagga acgcggagag gaacgccgcc gccacgcaaa aagagagcga cagcagcatt
     3841 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     3901 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     3961 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     4021 ccggggagca gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     4081 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     4141 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     4201 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     4261 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     4321 gaaagagatg cggaaaatga agccttgatc ccttttttct tctttttgtc tgctatcagc
     4381 gtagttagcc ctctggtatg atgagtccaa ctttgttttg ctgtgttatg gaaatctcac
     4441 tatggtcatt aaggcgcaaa gcccggcggg tttcgcggaa gagtacatta ttgaaagtat
     4501 ctggaataac cgcttccctc ccgggactat tttgcccgca gaacgtgaac tttcagaatt
     4561 aattggcgta acgcgtacta cgttacgtga agtgttacag cgtctggcac gagatggctg
     4621 gttgaccatt caacatggca agccgacgaa ggtgaataat ttctgggaaa cctctggttt
     4681 aaatatcctt gaaacactgg cgcgactgga tcacgaaagt gtgccgcagc ttattgataa
     4741 tttgctgtcg gtgcgtacca atatttccac tatttttatt cgcaccgcgt ttcgtcagca
     4801 tcccgataaa gcgcaggaag tgctggctac cgctaatgaa gtggccgatc acgccgatgc
     4861 ctttgccgag ctggattaca acatattccg cggcctggcg tttgcttccg gcaacccgat
     4921 ttacggtctg atccttaacg ggatgaaagg gctgtatacg cgcattggtc gtcactattt
     4981 cgccaatccg gaagcgcgca gtctggcgct gggcttctac cacaaactgt cggcgttgtg
     5041 cagtgaaggc gcgcacgatc aggtgtacga aacagtgcgt cgctatgggc atgagagtgg
     5101 cgagatttgg caccggatgc agaaaaatct gccgggtgat ttagccattc aggggcgata
     5161 atcccttccg tttaaagagc aaacccctca aacgaggggt tttttgttgt ttttacagat
     5221 ttcccattct tggcgggcaa cgttccagca actcgatgct gccgtcttcg ttttgctgtt
     5281 cgagcatcac atcaaatccc cacaggcgat gcacatgctt cagcacttct ttgcgccccc
     5341 gatccagcgg tgcgcgatta tgtggaatgt aacgcagcgt cagcgaacgg tcgccgcgca
     5401 aatccacgtt ccagatctga atattcggct ccagattact taagttatat tgcgacgata
     5461 accggttacg gatctcccga taaccttctt cattatgaat agcggaaatc tccagataat
     5521 tatgccgatc atcatccagc acggtgaaga agcggaaatc acgcatcact ttcggtgaca
     5581 gaaactggct gataaaactc tcatctttga aatcacgcat cgcgaaatgc agcgtttcca
     5641 gccagtcgga accggcgata tccgggaacc agtatttgtc ttcttccgtt ggcgactgac
     5701 aaatccgttt aatatcctgg aacatggcga acccgagggc atacgggttg atgccgctgt
     5761 accacgggct gttatagggg ggctggaaga ccacattggt gtggctgtgc aaaaactcca
     5821 gcataaaacg ttccgttact ttcccttcat catacagatg gttaaggatg gtatagtgcc
     5881 agaaggtcgc ccagccttcg ttcatcacct gagtctgttt ttgcggataa aaatactggc
     5941 tcaccttacg cacaatacgc aggatttcac gctgccatga ttccagcagt ggcgcatttt
     6001 tctccataaa atagagcagg ttttcttgtg gttcggacgg atagcggcgc gcttcagcaa
     6061 ccgttttctc ttcctcgcgc ttcggcaggg tacgccagag catattgacc tgactttgta
     6121 gatactcttc gcgacttttc tgccgggctt tctcttcttg cagtgagatt ttttgcgggc
     6181 gtttgtaccg gtccacgccg tagttcatca gcgcgtggca cgagtccaga agccgttcta
     6241 cttcatcaac gccataacgt tcttcgcact cggtaatata tttacgggca aaaatcagat
     6301 aatcgacaat cgaactggcg tcggtccaac tgcggaataa gtaattgttt ttaaagaaag
     6361 agttatgccc atagcaggcg tgtgccatca ccagcgcctg catggtaatg gtgttctctt
     6421 ccatcaggta agcgatacac gggttagagt taatgacgat ttcataggcc agtccttgct
     6481 gaccgtgctt atacagccgt tcagtttcga taaacttttt accgaatgac cagtgcggat
     6541 agttaattgg catgccgacg ctggagtagg catccatcat ctgttctgag gttatcactt
     6601 caatctggtg cgggtaggta tccagccggt agagtttcgc cacccggtct atctctgcca
     6661 gataaacatc cagcaggtcg aacgtccagt cgggtccatc gctcaaacgt gtggtgtcct
     6721 tattcataga atcgatcgtc gccatacgcg cacctcattg ttgtcggcgc tctctgtgtg
     6781 gagcacctca tttcaagcat agaacacctg ttaaaaaccg cgtcgccgga gaattttttt
     6841 ctttgcgatt tcttattatc agagtgccac taatccgctt ctgaacggaa ttttatgctg
     6901 gataaaaagg gcgttcagca ggagatacta aagacgccat attgccgcag agtcagggag
     6961 atgtgagcca gctcaccata aaaaagccgt atgttgaata atattttcaa ctgagttatc
     7021 aagatgtaat tagattatta ttcttttact gtatctaccg ttatcggagt ggctatgcga
     7081 gttgtcatac tgggaagtgg tgtggtaggc gttgccagcg cctggtactt aaatcaggca
     7141 ggacatgagg tcaccgtcat tgatcgggag ccgggggccg ccctggaaac cagcgccgcg
     7201 aatgccgggc aaatctcccc cggatatgct gcaccgtggg cggcaccagg tgtgccttta
     7261 aaagcgatca aatggatgtt ccagcgccat gcgccgctgg cggtccgtct cgacggtacg
     7321 cagtttcagt tgaaatggat gtggcaaatg ttacgtaact gcgacaccag ccactacatg
     7381 gaaaacaaag ggcggatggt gcgtctggcg gaatacagcc gtgattgcct gaaagcattg
     7441 cgcgccgaaa ccaatattca gtatgaaggg cgtcagggtg ggacgctgca actgttccgt
     7501 accgaacaac agtatgaaaa tgcgacccgc gatatcgccg tgctggaaga tgccggcgta
     7561 ccgtatcagc tgctggaatc cagccgcctg gcggaagtgg agcccgcgct ggcagaagta
     7621 gcgcacaaac tgacgggtgg tctgcaactg cccaatgatg aaaccggaga ctgccagctg
     7681 tttacccaga atctggcgcg gatggcagag caggcggggg ttaaattccg ctttaatacg
     7741 cccgttgacc aactgctttg cgacggcgag caaatctacg gcgtgaagtg tggcgatgaa
     7801 gtgattaagg ccgatgcgta tgtgatggcg tttggttctt actcgacggc gatgctcaaa
     7861 ggcattgttg atattccggt gcatccgctg aaaggctact cgctgactat tccaattgcg
     7921 caggaagatg gtgcgccagt atccactatt cttgatgaaa cctacaaaat cgccattacc
     7981 cgtttcgata accgcattcg tgttggcgga atggcggaaa ttgttggttt taacaccgag
     8041 ctgttgcaac cgcgtcgtga aacgctggaa atggtggttc gcgatctcta tccacgcggc
     8101 ggtcatgtcg agcaggcgac tttctggacg ggtttgcgcc cgatgacgcc agacggtacg
     8161 ccggttgtcg ggcgtacacg ctttaaaaat ctgtggctga ataccggtca cggcacgctc
     8221 ggctggacga tggcttgcgg ttccggtcag ttgttaagcg atctgctctc tggtcgcacg
     8281 ccagcgatcc catatgagga tctaagcgta gcgcgctaca gccgtggatt tacgccatca
     8341 cgtccgggcc atttacatgg cgcacacagc taaggaaacg agatgacccg tccgatacag
     8401 gccagcctcg atctgcaggc attaaaacag aatctgtcca ttgtccgcca ggccgcgccg
     8461 cacgcgcgcg tctggtcggt ggtaaaagcg aacgcttacg ggcatggtat tgagcgtatc
     8521 tggagcgcgc tcggggccac cgatggcttt gcattactta acctggaaga ggcaataacg
     8581 ttacgtgagc gcggctggaa ggggccgatc ctgatgctgg aaggattttt ccatgctcag
     8641 gatctggaga tttatgacca gcaccgcctg accacctgcg tacacagcaa ctggcagctc
     8701 aaagcactgc aaaatgcgcg gctaaaagca ccgttggata tttatcttaa agtgaacagt
     8761 gggatgaatc ggttgggctt ccagcccgat cgcgtgctta ccgtctggca gcagttgcgg
     8821 gcaatggcga atgttggcga gatgaccctg atgtcgcatt ttgccgaagc ggaacatcct
     8881 gatggaattt ccagcgcgat ggcgcgtatt gagcaggcgg cggaagggct ggagtgtcgg
     8941 cgttcgttgt ccaattcggc ggcgactctg tggcacccgg aagcgcattt tgactgggtt
     9001 cggcctggca ttattttgta tggcgcttcg ccgtccggtc agtggcgtga tatcgccaat
     9061 accggattac gtccggtgat gacgctaagc agtgagatta ttggtgtcca gacgctaaaa
     9121 gcgggtgagc gtgtgggcta cggcggtcgc tatactgcgc gcgatgaaca gcgaatcggc
     9181 attgtcgccg cagggtacgc cgacggttat ccgcgccacg cgcctaccgg tacccctgtt
     9241 ttagtggacg gcgtgcgcac catgacggtg gggaccgtct cgatggatat gctagcggtc
     9301 gatttaacgc cttgcccgca ggcggggatt ggtacgccgg ttgagctgtg gggcaaggag
     9361 atcaaaattg atgatgtcgc cgccgctgcc ggaacggtgg gctatgagtt gatgtgcgcg
     9421 ctggcgttac gcgtcccggt tgtgacagtg taacttgttg taagccggat cggaggcagc
     9481 gtcttctggt tgcgaaaaaa tcatccatcc ggctggtcag caactgtagt tgttaatgtg
     9541 acagagccat tgcccatgat agtgtccatt aaaagaatgg acactatttc cccggaacct
     9601 gaactcaccg cacaggcgtt ctacataaaa cgcttacgct tcattgttga ctcgaactcg
     9661 acttcagata aatcacgctt tacccttgat ggagcctgta catagatttg tgtaattgcc
     9721 tgattttgat atgttcaatt caacatcaaa tgaaggttaa attatggacg acaaacaatt
     9781 gcaggctcag gctgcgttca gcaaagcatc gcaaccggcg atagatgctt cattaaattt
     9841 aagattcagc ttcctcttca gccacccgta cgccaatctt caacacttca ttatcttctt
     9901 tctcggccac cgtccagatc atcccggcaa actctacctg gtcaccgaca accggtgccg
     9961 cgcctaacaa ctgctggaca atttcaccca gcgtttgctg cttatcacga tattctcgcc
    10021 cgtcttccag gccatatatc agcgcaacat cagcatattt ggcgctggct tcgagaatga
    10081 agtcaccaaa gaagcgttga tccagcgcga ccggcggcga ctggctgaac agtttaccga
    10141 gcgccggaag atcgcgttcc cgaccaatta cacacaacac atcgccttcg cgcagtcggg
    10201 tgctgccggt aggatgaagc aactggttat cacgaaacag tgccgcaata cgcgtctctt
    10261 ttggcatatg caaatcgcgc agtgccgcgc ccacgcacca tttatcggca ctcaattgat
    10321 aaacaaactg ctcccacgga ttttccggat gaatatccag gccaacgcgt gacaccggac
    10381 gtcccactgg cggaaccacc actttggctt ttttagccgc ccacgagagt gatgttccct
    10441 gcaagagcag tgaaaccaga accacaaaga aggcgacatt aaagaacaga cgtgcattct
    10501 ccagccccgc catcatcggg aacacagcca ggatgattgg caccgcgccg cgtaatccta
    10561 cccagctgat aaacacgcgc tcacgcagat tgaaaccgcg gaaggggagc aatccagcaa
    10621 ataccgaaag gggacgggcg aagaatatca tccatgccga caaaatgagc gccggaatgg
    10681 caataggcag cagatcgctt gggttaacca acagccccag caccaggaac atggcgattt
    10741 gcgccagcca ggcgaggccg tcgaaatttt gcaggatgcc gtagcggttg cgaatcggcc
    10801 gattacccag cagaaaaccg cacagataaa cagccagaat accgctgcct tccagcgccg
    10861 tggttaaagc aaaaattaaa ataccgccgc ttaatgccag caatggatat aatccggcgg
    10921 gcagggcgat gcgattaatc atttgcagca gtaaataacc accaccaagt ccgatgacaa
    10981 tcccgaggcc aaattgctgc agaatatcga ccacgaacat ccaactgaca ctgctttcat
    11041 gttgctggat catcgcaatc agggtaatcg tcagaaagac cgccattgga tcattactgc
    11101 cggattcaat ttccagcgtc gagccaacac gttcgttaag ccccttacca cccagcagag
    11161 aaaagaccgc tgcagcatcg gtagagccga cgatagcgcc gattaataag ccttcaatca
    11221 aatcaagatt aaacagccac gccgccatca tgccggttaa accagaggtg ataagcacgc
    11281 ccagcgtcgc cagcgacagt gccggtccta acgccacacg aaaggaactg gcctgagtgc
    11341 gcatcccgcc gtcgagcaaa ataatcgcca gtgccaggtt acttaccatg taggcgaagg
    11401 ggtaattatc aaacgggata ccgccgacgc catcgactcc tgccagcatg ccgatcgcca
    11461 gaaaaataac cagaatagga atgccaagac gggaagaaaa tgaactaagt aatatactgc
    11521 tggtgacaag gatggatcct aagatgaaaa ggctaattat tgttgtggca tccaacggtc
    11581 ggttactcct gattacgctg tctcttatat aaaccctacc atattagcgg cagagacagc
    11641 gttttactta gccctgaagc gtgatttttt tacattttaa gaacaggatg accgctaatc
    11701 gtcaactgag tgccttcccg ggtgttattc agaatggcat gtgcgcccaa cggcagcgta
    11761 accgtgcgtt gttcatgacc aaaatcgagg ccggtaatga gtggaatcga caggcgggaa
    11821 cgcaaaaacg cgtacactga ctccaggttg taacctgcgt cataatcatt gggcgtgctg
    11881 ccgctaaagc taccgagaat aatcgccttc tgacgtggca aaattcccgc atgatagagc
    11941 tgcaacagca tacgttcgac gcggaaaggg tgctcgttaa tatcttccag caccagaata
    12001 ccgttctcaa tttttggcat ccacggtgta ccaatcagtg aaatcagcat cgcaagattg
    12061 cctccccaca acgtgccttc ggtctgacat gtcgggcctt ccccttgcca ttcaatggtg
    12121 aaggtttcat tgcgtaacgc cagccagaaa tggtgctcgg taaaggcgtt cagttcatcc
    12181 gcgccaaagt ttgctaccag catggggccg ctaaaggtga tgacattgcc ttgcgccaga
    12241 agaccgcact gaatggcggt aaaatcgcta tgcccgcata tgagcaacgg gtcatattgt
    12301 tggcgagcaa ccagtgcctg ccagtcaata tctgccagta aacgactggc accgtaaccg
    12361 ccgcgtacag ccagcacgat ggtgttgggg gtggtcagtc tggcaaggga attgagatct
    12421 tccaggcgct ccgtttccgt acccgcaaaa cgctcacaac gacgggcaat aacctcgacg
    12481 ttattgacct gatgcccctc gtcggtaagg cgctggatac cacgcagcgc ggcgtgctgt
    12541 ttaatgcagt aacccgatgg ggcaattaag tgaaacagag acatggcaat tccttgctga
    12601 caacagaaac gaaatgtata tcatgccgct taggtgtgcc gttgtcacct caacggcgat
    12661 tccaggctat aaggatagaa gaagtgaaat tgagatggtt tgcctttttg attgtgttat
    12721 tagcgggttg ttcatcaaag catgactata cgaacccgcc gtggaacgcg aaagttccgg
    12781 tgcaacgtgc gatgcagtgg atgccaataa gccagaaagc cggtgcagcc tggggcgtcg
    12841 atccacaatt gatcacggcg attatcgcta tcgaatcggg tggtaatccg aatgcggtga
    12901 gtaaatcgaa tgccattggt ttgatgcagt taaaagcttc aacctccgga cgtgatgttt
    12961 atcgccgtat gggctggagt ggtgagccga cgaccagcga gctgaaaaat ccagagcgta
    13021 atatttcaat gggggcggct tacctgaata ttctggaaac cggcccgctg gcaggcattg
    13081 aagatccgaa ggtactgcaa tatgcgctgg tggtgtcata cgctaacggg gcaggtgcgc
    13141 tgctacggac tttctcgtca gatcggaaaa aggcgatcag caaaatcaac gatttggatg
    13201 ctgacgagtt cctcgatcac gtagcgcgaa atcatcctgc gccgcaggct ccgcgctata
    13261 tctacaaact tgagcaggca ctggacgcga tgtaaatcag tcgcgcactt tgtccgcttt
    13321 ttcccgggct tctcgctcga gagagaaaat aatccgctgt aattgccgct ccaccgtcgg
    13381 gctgacgtta agaaaacgga agctcagacg gggagtggtg atggtttcat tcttgccatc
    13441 aatcactttg cgctcgctga tggagattaa ctgggcgtca aagtgaaaaa caccccattg
    13501 ccccatgttg acttcaatct gagcgaagcg catgccttct tgtaattcgg caggctttgc
    13561 tgtttccagt aatgcgccca tgccgcctaa cgacaaatca tacaggcgga aacgtaacgt
    13621 actgttatcc gccagtttgg tctggcaaaa ataaggcgga tggagtgggg cggagatgcg
    13681 gaaatatcgg cgtcgttgta caaaccataa ggtgggaggc ggtacggtga taaatgccgg
    13741 aagctgcaag tattcactct gctgtagttg ttcaacagta aactcgactt tcgcaccctg
    13801 agtttcggcg gtaatggtaa tgtgctgtgc ctttagcacg gcgatgttgt cttcggcttg
    13861 actgccgaaa tccagcacca gtttatccgg ggttattgcc agtattttgc tgatcagctg
    13921 cccgccattc caactgagac gcaaaggaat tgcggctttg tgcaaatcgc gtaacacgcc
    13981 caggacggct aacggatttt gtttcaggaa ctgctcatgg taatgactca cgcggaaaaa
    14041 ctcctgactg acaaactgtc tttggtttat cggtcacagt taacaaaact taatacaaat
    14101 gcgtgaatat tttttacatg ttgttcttaa atcagccgac atacgcccag catcgatccc
    14161 gcccttgcct atacttagag cgttgacgta agcatttctt gcgtccgatt catcgaaacg
    14221 agggcctgaa catgggaatt attgcctgga ttatttttgg cctgatagcc ggcattatcg
    14281 ccaagctaat catgccgggg cgtgatggtg gtggattttt cctgacctgt attctcggga
    14341 tagtcggtgc ggtggtcggc ggctggctgg cgaccatgtt tggcattggc ggctccatca
    14401 gtggttttaa tctgcacagc ttcctggtgg cggtggtggg agctattctc gttctgggcg
    14461 tattccgcct cctgcgaaga gaataagatt ttcataaggc ggatagcgat acagatgccg
    14521 ctatccgctt tcacatcaga acgtatactc gacacctgct ttacgggtgg aacaaatcaa
    14581 tatagcactt agatgcccga taaagtcggt tcccggtaac aacaacagaa tcctttttta
    14641 attattgttt cgttgttttt ggtgtgattc agaaaataaa tattttagac agtagccagt
    14701 caggcgttaa aaatggggcg gatagtgagg cggttttggc tgcgcttatt gaacatttag
    14761 aattgatcaa tccttctggc agattaagcc aaaatacccg ttcggcaatg cttcagttac
    14821 gcgaagagga atggtctgaa ttcttttttt ggttgctaaa ttctctggag tgtctggatt
    14881 atgtgattat taatttagct ccagaaagca aaaaaacgtt gatgagtgaa caccgtaaca
    14941 atattcaggt agcaatcgat gctttatata ggcaacgtcg gtgtaaatca ccgggtgatg
    15001 aaagtgaaac acttacccgt agaaatgacg ctatcttcgg caaccacgta tggcaaacat
    15061 ttgcgcaata cttcccgcca ggacttgaaa aaccgtctgt gtagaaaaca ccatagctat
    15121 ggtgttatct gccgacaacg ccatccggag aagccgaatg acgttgtcgg agtaagcgct
    15181 ggttaaggtg tgggttgtgc ctctttggtt gagggttgcg tcgttgctga cttaacggtc
    15241 ggacgcgtcg ccggaacatt gtcacacggt tgctctttcg ggcagatcaa atccagcatt
    15301 ttcagcgtca cgccattggt ccagccaaag ccatcctgta atggatattc gccaccgccg
    15361 ccccccgttc cggtggtgct gacatcatat ttttccacca gctttttctc ccggtcatag
    15421 gtgtgctgaa cattggtcag gaagtgccag ctaatgtcca tcgccacctc tttttgcccg
    15481 tagttttgta atccttctgt cgcgacccac tgtaacggtg cccagccatt tggcgcatcc
    15541 cattgttgcc cacttttcac cgacgtggtg ttcaggccgc cgggttgcag cagatgtgtt
    15601 ttcgtcgccg tcgccatttt gttggcgcga tctttcgctg ccgcattgac gtacagcggg
    15661 aacagggcgg ccgcggttaa ctgattgcgc actttatgac ttttcaggtc gtaatcggca
    15721 taccagcctt gttgatcgtt ccacatgtat ttttcgatcc ctttttgacg ggcatttgcc
    15781 agcgtttcgt actggtttgc catcgcgtta tctccggcag ctttgctggc gcgggcgagg
    15841 attttttcca ttttaaacat caggctgttc agatcgaccg gtacgatgct ggtggtgcgt
    15901 aaggtattta gttgatgcgg gttgtccatc cagcgcgagc tgaaatccca gccagacgca
    15961 gcggcagagc gcaggtcgcg gtaaatttca gtggcaggtc gattcggatt gcttttggcg
    16021 gtggcaatat cttccaccca tgactctggt cgtggcgtat cgcgatcgtc ccagtagcgg
    16081 ttgagaaggg taccatcctg aagttttaca acgcgttttt cctgttgtcc ggcttgcagg
    16141 ttttcaacac cgtccatcca gtaagcatat tctttttgca tttgcggcag gtattgcttc
    16201 aacgcggcat cgccttcatg ctgcgccagt aactctacca tcagggcaaa gaagggcggt
    16261 tgcgagcggc ttaaatagta actgcggttg ccgttgggaa tatgaccgta agtgtctatt
    16321 tcatgagcaa aattggccac catatccgcg actttatccc agtgaccgct ttcggcaagt
    16381 cctaacatgg tgaagtaact gtcccagtaa tatacctcgc gaaaacgtcc gcccggcacg
    16441 acataaggtt ccggcagcgg taacagagaa tcccattttt cggtgttttc ggtagaacgc
    16501 gttaataccg gccaaagtcc gtcaatatgt tcgcgcagtg actgcccctc tggcggaaca
    16561 tatttctcgc cctctttcgg cagggtgaaa ttgacgttaa cgaaatggcg cagatcaaat
    16621 ccgctctggt tttgctgcat ccgataatca gcaaggatca tcagcggatc gctgttcggc
    16681 acggcatcgg caaaggtttt ttggtccgga aaaagtttgg cgttttgcac atcattaaac
    16741 agcggcccta ataaaatatc aggcggctgt ggtgttaccg gtgtttcttc tgcctgcacc
    16801 gatagcgcag cgaaacacaa aaagatacag gctggaatta acgccatttt ttgcgggcga
    16861 gaaggtgcgg gggatttcat caatcattct cctttggcga aaccgaataa aacggtattc
    16921 aagtatcaga aaaccttagt tcaggatcgc actagctgca tgatcgaaat ctcattctgc
    16981 gaactgtcag gcattttaga aaagggaatt aaagggataa ataacggtga aatagagtga
    17041 tcaaataacg tctgtttatt agccagtcag cgacgttggg ttcgcgatta gcgaagaaaa
    17101 atcatcataa attggtaaaa aatataacag gtagttaaaa aacatcagtg ctgggtaaat
    17161 tgccggatga catcagaacg atgccatccg aacggtggct taaccctgac ggttgaaacg
    17221 ttgcgtttta acgtccagcg ttagcgtttc ttctggttgt atcgcataca gtttctcacc
    17281 ctgctggcaa atccagccaa tccccagttc acgggcgatt agggcgctgt gggataccgg
    17341 acttccggcg ctaaggcaga taccttttac aaccgccgga tccagttgca gtactgtgga
    17401 aggataaatg ttctccgcca gtagaatagt tggcgagtta aactgcggga gttcttcttt
    17461 cgtttgggtc aggtggacca gggtgcgatg cagaagatcg tccacatcaa tatagcgagc
    17521 ttgtagatat tcatcatcca gttgctggta ttgctggcta agttctttaa gaacttgctg
    17581 ccaggcatat tctgccgtgc aatgttcatg ctgaaggagt tcgcttgccg ccgccagcag
    17641 ttccggatca tctaacagtg tatggtgacc agaaaagatt gcggcaatat cgtcaagccc
    17701 gctggcttct gctttcgctg ttaacgtcat cagatctaat aacgtgaagt caatagcctg
    17761 gcgtaatcga tcttgttctt cttccacggt cagggttgat tttgcctgta ccgtacataa
    17821 aactggttga taataaaagg ctttacccga aacaggcgga acgggacgca gagtaggtgg
    17881 agcgacttcc tccgtttcac caaagttatc ttcagccagc tgacggaaag cgatcagtgc
    17941 ctcttcagct tctggccctt tcgcaatcag gcgcagcgta tcgttatagc gaacttgtag
    18001 taacgcaatc tggttaatac tctctggtgt gacgcatttg ccgttttttt ccagcaacat
    18061 atcggcatta aatgtcgata aggtataaac cagccgggag gccggacgta catgcaggcc
    18121 gttacggttt tttatgacca ccgccagaga acgggcttct tcatcgtacg caggacatgt
    18181 gtcagagatt tcagtgtcgg aggacggtaa acccagttgt tcacgtttgg cttccagcgc
    18241 atgcatagcg tcaaagataa ctttgtcgat atccgctccc gaggccgcgc tgaccgttgc
    18301 tgccagtgta ccttcgacca acggcgcagc acacaaacgt acttttgcgg cgatcttggg
    18361 agccagcaat tccagcgcag tttcagcact caataatgcg ctacccatat ccatcatgac
    18421 cagcacatgg tcggcatcag caacagattc gatggcctcc atcactttga cggcatcggt
    18481 accaatagga ttttgtggat cgtcaattcc cgcggcaatg gcgattttac aactatcact
    18541 cattaacatc tgacgggcta attcaccgac accttctccc agtcggctgc tatgtgaaac
    18601 tatgaccagg tttaccatca ccaattcctt actcttttgc ggctaacgcc aacatttgca
    18661 tcataaacat caccgaggtc gcgccgggat cctggtgacc aatactgcgt tcaccgagat
    18721 aactggcgcg gcctttgcgg gcttgcatcg taatcgtact ttgtgcagcg gattcggcga
    18781 ttctactggc agcttcgagc gccaccggaa cagagagatt ttgctcgctg gactgacgta
    18841 acgattccac caccggcacc cacacatcac acatggtttt atcgccaggt tcggctttcc
    18901 cgcgactgat tacgccgtcc gcgccatcgc ggaacatctg ataaagctct tccagtgtca
    18961 ggctttgccg tgcctgggtc gcctgtgcgg cgcggataaa gaaggtaccg aacagcggac
    19021 cactggcacc accgacgctg gaaagcagcg tcataccggt attcttgaga atgaaaccga
    19081 tatctttatc tgcgatagca gggagttttt ccaccacttt gctaaagcct cggttcatat
    19141 ttagcccgtg gtcagcgtcg ccaatttcgc gatccagtcc ggtaagatac tcgctctcgg
    19201 tgctgaaaat atcgccacaa cgagtgagcc agttaacaat ttgagttctg ctcagtgaca
    19261 ttgctttctc cttatttacc ccagttaagg gccggggtgt ggaccggggc gtcccagagt
    19321 gccagcgttt cgtcatcaac tttcagtaag gtgattgaga aaccggtcat atccagtgag
    19381 gtgcagtacg cgccaattaa attacgttcg atagtcaatc ccgcttgctg gcaacgtgtg
    19441 gtcaggcggt tatagacgcc gtacagctca gaaagcggag ttgcgccaag attgttaacc
    19501 agcgcaatca cccgatcgcc agactggagc ggttgtttgg tttgttgttc ttcctgccaa
    19561 ctgccttgtt gataatccca gaaacgcaaa gtgcgatggt atgagccatt ttccagcagg
    19621 gtgtcgaaca tttcatcgac ggtttgatca agggaagaga aggagcggcg gtcaataccc
    19681 ggctcaccat gaatgccgac gccaaactcc atctcattat ccgccagggt aaaagaaggt
    19741 ttgcccgcgg caggaacggt acaggcaccg agagcgatac ctattgagtg gccttgatta
    19801 ttcaacttac gccccagttc cgcacaggcg tccagtgagt cgccacgctc cgccgctgcg
    19861 cctacgagtt tttcaattaa tacggtgttg gcaacgccgc gtcgcccggc ggtataaaga
    19921 ctgtctttta ccgcaacgtc gtcatcaatg accacagtgg tcacttttac gccgctatcg
    19981 tgcagtaact cggtcgctgt ttcaaagtta agaatatcgc cggtgtaatt tttgataatc
    20041 aacagtacac cttcgccgcc atcaatttgc atggagcatt caaagatttt atcgggcgtc
    20101 ggtgaggtga aaatttcgcc cggacaggcc cccgaaagca tcccctgacc gatataacca
    20161 cagtgcatcg gctcgtgtcc gctgccgcca cccgacagca gggcgacttt tcctgcaaca
    20221 ggggcatcag ctcgggtgac atacaccgga tcctgatgca gtgtcagcga tggatgcgct
    20281 ttcgccagtc ctgccagttg ttcgtccagt acgtcttgca catcattgat caattttttc
    20341 attattttgc tccagcaatt acggtagggc atggatgatg ttcaacgaca cggcgaccac
    20401 tgactgccga tgaatccatt gtgcatcagg caagggaaaa gaaaattagc ccaaattatg
    20461 tttcatagtg aaacatatgc tttaatgaat gttccatatt gaaactttta cgtgtattaa
    20521 tacttaaaat tgcgagccgg aacacctttt gtcataaggg atgcgggata tgagtggcgc
    20581 ttttaacaac gatggtcggg gcatatctcc cttaattgca acctcctggg agcgatgcaa
    20641 taagctgatg aaacgggaga catggaacgt accacatcag gcccagggcg tgacatttgc
    20701 ttctatttat cggcgtaaga aagcgatgct gacgctcggg caggctgcgc tggaagatgc
    20761 ctgggaatat atggcaccgc gagagtgtgc gctgtttatc ctcgatgaaa ccgcctgcat
    20821 tctcagccgt aatggcgatc cgcaaacctt gcagcagcta agtgcactgg gattcaatga
    20881 cggcacgtat tgcgccgagg gaattattgg tacttgtgcg ctatcgttag cggctatctc
    20941 tggtcaggcc gtgaaaacga tggccgatca acatttcaaa caggcactct ggaactgggc
    21001 cttttgtgca acgccgttgt ttgacagcaa gggccgattg acgggaacaa tagcgctggc
    21061 gtgtccggtt gagcaaacta ccgcagctga tttgccgttg acgttggcaa tcgcccgcga
    21121 ggtcggaaat ttactgctga cggacagttt gctcgctgaa actaaccgtc atttaaatca
    21181 acttaatgcc ctgttagaaa gtatggatga tggcgtgatt agctgggacg agcagggtaa
    21241 tttgcaattt attaatgccc aggcggcgcg ggtcttgcgc cttgacgcga cggcaagtca
    21301 gggaagggca atcactgaac tcttaacgtt acccgccgta ttgcaacaag caataaaaca
    21361 ggcacatccg ctcaaacacg tagaagcaac ctttgaaagt cagcatcagt ttattgatgc
    21421 ggtgataacc cttaaaccga taatagaaac gcagggaacc agctttattt tgttgctcca
    21481 tcctgtggaa cagatgcggc agttgatgac cagtcaatta ggaaaagtca gccatacctt
    21541 cgctcatatg ccacaggacg atccgcaaac ccgccgcttg attcattttg gtcgccaggc
    21601 ggcgcgcagt agctttcctg tcctgctttg tggagaagag ggcgtgggaa aggcactgct
    21661 aagtcaggca attcataatg aaagcgagcg tgctgcaggt ccttatatcg ccgtcaattg
    21721 tgagttatat ggtgatgcgg cgctggcgga agaatttatt ggtggcgatc gcacggacaa
    21781 tgaaaatggc cgtctgagtc ggctggaact ggcacacggc ggcacgctgt ttcttgaaaa
    21841 gattgaatat ctggcggtgg agttacagtc tgctttgctt caggttatca agcagggtgt
    21901 tatcacgcga ctggatgcgc ggcgtttaat accagttgat gtcaaagtga ttgccacaac
    21961 gaccgcggac ctcgcaatgc tggtggaaca aaatcgtttt agtcgccagc tgtattacgc
    22021 gttgcatgca tttgaaatta ccatcccgcc tctgcgtatg cggcgtggca gcattccggc
    22081 gctggtgaat aacaaattac gcagtcttga aaaacgcttc tctacgcggc tgaaaattga
    22141 tgacgatgcc ctcgctcgcc tggtttcctg tgcatggcct ggcaacgatt ttgaacttta
    22201 cagcgtcatc gagaatcttg ctctgagtag tgataatggg cgcattcgcg tcagtgattt
    22261 gccggaacat ctgtttaccg agcaggcaac agatgatgtc agcgccacac gcctttccac
    22321 cagtctgtca tttgcggaag ttgaaaaaga ggcaattatt aacgcagccc aggtcacagg
    22381 cggtcgcatt caggaaatgt cggctttact tgggatcggc cgcactacgc tgtggcggaa
    22441 aatgaagcaa catggcattg atgcagggca gtttaagcgc cgggtatgaa agacagagac
    22501 gatttctgat acatcagagt gatctatatt tcattccggc gcacgctaac atttttcagc
    22561 atcgtttaag ggctggtcta tcccgcactt aaaagctgaa gcgatatcct ccgttgactt
    22621 gcttctgatc aaagaggtta ctctgcgtgt aatccgcttc gaggtagaat gtgtgctgtt
    22681 tgttatactg tgcactgact cccacgccgt tattccagcc attacctttg aaactgtact
    22741 tctcccggga gttgttcaac agatagtctg tatcccctga aaactcgcgg atagctccag
    22801 tcttcacata catattcagc tggctgttgc ctgcggtgat gtcatatccc agtatcatgc
    22861 tggcacgccc cagcagcgat tcgtagtgat tcagatgtat attgagacca ttactcgcct
    22921 tcatagccat ctcattctgg tggctgtatg tgagctgggt ttgcggctct atatagaacc
    22981 cataaccagt aggggtcagg ttgaacctct gcccggcttc cagggagatg ctcaatccat
    23041 tcgcagtgcc gttggcgtta acgccgttgt tctgactgtc cagtacgtga aaactatttt
    23101 tctggcgcga tgcttttaca acgagatcgc tgtaaaaacc gttgtgtgcc atgtaactgg
    23161 catacattcc catgtagtct gaacgtgcgg taccgtcgcc tccgctatag tccggcgatg
    23221 catgtgttga gccaatatac agaccgacat acaacggcat tacatcagag agacgtttat
    23281 ccccaccaaa ctggataccg ctgtaaccca tgtcaaagcc gctcagtttg ccactggcga
    23341 aggagtccag gcttccccca taactgcgca gccagatatt accgtcttta ctctgatttc
    23401 gcaggtcacc cattcgttgc atcagcgtac ggttttcaac atagtttaat aagtaaccga
    23461 cattgagata attgccgcca gcatctgcgg tcgtggtggg tttaggtgtg ggagtggggt
    23521 taggcgtagg atcgggttca ggcgtaggat cggggttgac tatgggaggc tgagcgggag
    23581 ccggtgttgg ttcaggatta ggagtcggtt ccggaactgt cccggaagcg taaagctccc
    23641 agttagtgcc atttttacgc acatcgtaca gatatccccc caactcaacc tgagaagacg
    23701 cgctgaacga ggccgcaccg tcagtggttt ttaccactgt cagaacttca tttcccgtag
    23761 tggcctcgct gccctggttg cggatagcca atacgtgatt accagcacta cttccgctga
    23821 tattcaataa atccccttta ttattaacgc cattaccctc gccaacaaca tcagcacgca
    23881 taataaaggt actgttaccg ctcaggttct ctacgtttaa tgtggcaaat gtgccggcag
    23941 ttgacccgtg gctggcaaaa tcgacagttg aatggctcag cgccagcgtg tcgagattag
    24001 aattacttgt tacgttccag acgctgttat tcattgcaac gtccagtttc ccgccattga
    24061 cattatcgct gagggaggaa cctgtccaaa ccgaccctgg gtgcatatct agattgatta
    24121 gcccaccttt ggaaagaacg ctaccgttga taagcatacg acccgaggcg ttgatgcggc
    24181 tggcggcata accatcgtca tgttgcgttg caatggccat ctggtcgggt gtactcatat
    24241 caatgaccag atcgctcgtg aggtcgatct ggctgttggt catggcataa attcccctgg
    24301 ctcctgccgc gccggtgata gccaaactgt ctccggttat tctaccgccg ctgagcgccc
    24361 acaaacccag cgccagactg ccattacgat caactgtaat atcggtattt tgcatgttga
    24421 caacagccgt tgccgtctgg gccgaggcac cataagaacc gccggaaaag atgctgtttc
    24481 gttgcgctgc cgtgccagaa aaattgattg tcgcgtctga accactggtg acgaggccac
    24541 cgccctgcgc ggaagaaata tggctatctg caccgatagt ggttgtacca ccacgaactt
    24601 cgacgccatt ggccgcggct ccagttacat caacagtgag tgcattcgcg ctaacctggc
    24661 caaagctcca gaggccgtgt gcattatcgc cattggtttt aatggaactg tttgttccga
    24721 gatcgacaac agagtttttc tgtacgttta tccccatggc gctgtagccc tgaacatcga
    24781 ttgtcaggtc tgtagccgta aaacgcgcag caccattggc gttattgccg ttgagaccac
    24841 cgatataaac acctgtactt ccatcggtcg tgattttact tccgcttcca agatcgacac
    24901 tggtgccata gtcattgatg gttaggccta taccgttcga gttttcaatg gtgaattgag
    24961 tggctgttag tgttgagcta tgcccaataa tgatgccgtc atcgttactc ttaatggtgc
    25021 tgcctgtgcc taagtcagca tgggtatagt caccaattaa gttaatgccg atggcagagg
    25081 tttgaccaac aacatctact gtgagtcggt tagctgtcag ggagctgttt ttgccctgaa
    25141 taatgattcc ttttgcatat ccagaggcgt cgttgacatt gacggtgaca tcattaccca
    25201 ggttgatatt gccaggcgtg tttcctgctg gggtcattac accatagagg tttcctgatg
    25261 gatcggcggt atctcctgta attctgtcac cttcattaat agtaatattg gtgccatatt
    25321 gacttcctgt atcagcagct atcagcaggg gggaaaagat aattgcattc aaaccaatag
    25381 cgccaaattt tatcagttga attgagggcg aacggatttt taattcagcg agacgatctg
    25441 ctttggtatt cccattgtgt tgtttgatgc ccataccact cctatatagt acccagacta
    25501 tg
//
LOCUS       DAZFWL020000061        25379 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFWL020000061 DAZFWL020000000
VERSION     DAZFWL020000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.061"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            119..952
                     /gene="ppsR"
                     /locus_tag="ACHKNP_RS13230"
                     /old_locus_tag="pgaptmp_002646"
     CDS             119..952
                     /gene="ppsR"
                     /locus_tag="ACHKNP_RS13230"
                     /old_locus_tag="pgaptmp_002646"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HGU4704787.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKLLDIMGLSRRM
                     Y"
     gene            1109..2155
                     /gene="aroH"
                     /locus_tag="ACHKNP_RS13235"
                     /old_locus_tag="pgaptmp_002647"
     CDS             1109..2155
                     /gene="aroH"
                     /locus_tag="ACHKNP_RS13235"
                     /old_locus_tag="pgaptmp_002647"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HGU4704788.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLEMARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNSRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            2287..2478
                     /gene="hemP"
                     /locus_tag="ACHKNP_RS13240"
                     /old_locus_tag="pgaptmp_002648"
     CDS             2287..2478
                     /gene="hemP"
                     /locus_tag="ACHKNP_RS13240"
                     /old_locus_tag="pgaptmp_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HGU4704789.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2482..3918)
                     /gene="selO"
                     /locus_tag="ACHKNP_RS13245"
                     /old_locus_tag="pgaptmp_002649"
     CDS             complement(2482..3918)
                     /gene="selO"
                     /locus_tag="ACHKNP_RS13245"
                     /old_locus_tag="pgaptmp_002649"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HGU4704790.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(3981..4694)
                     /gene="ydiV"
                     /locus_tag="ACHKNP_RS13250"
                     /old_locus_tag="pgaptmp_002650"
     CDS             complement(3981..4694)
                     /gene="ydiV"
                     /locus_tag="ACHKNP_RS13250"
                     /old_locus_tag="pgaptmp_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HGU4704791.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            complement(4941..5405)
                     /gene="nlpC"
                     /locus_tag="ACHKNP_RS13255"
                     /old_locus_tag="pgaptmp_002651"
     CDS             complement(4941..5405)
                     /gene="nlpC"
                     /locus_tag="ACHKNP_RS13255"
                     /old_locus_tag="pgaptmp_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU4704792.1"
                     /translation="MRFCLILITALFLAGCSYHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(5483..6232)
                     /gene="btuD"
                     /locus_tag="ACHKNP_RS13260"
                     /old_locus_tag="pgaptmp_002652"
     CDS             complement(5483..6232)
                     /gene="btuD"
                     /locus_tag="ACHKNP_RS13260"
                     /old_locus_tag="pgaptmp_002652"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HGU4704793.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(6232..6783)
                     /gene="btuE"
                     /locus_tag="ACHKNP_RS13265"
                     /old_locus_tag="pgaptmp_002653"
     CDS             complement(6232..6783)
                     /gene="btuE"
                     /locus_tag="ACHKNP_RS13265"
                     /old_locus_tag="pgaptmp_002653"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HGU4704794.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(6846..7826)
                     /gene="btuC"
                     /locus_tag="ACHKNP_RS13270"
                     /old_locus_tag="pgaptmp_002654"
     CDS             complement(6846..7826)
                     /gene="btuC"
                     /locus_tag="ACHKNP_RS13270"
                     /old_locus_tag="pgaptmp_002654"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HGU4704795.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            complement(7927..8226)
                     /gene="ihfA"
                     /locus_tag="ACHKNP_RS13275"
                     /old_locus_tag="pgaptmp_002655"
     CDS             complement(7927..8226)
                     /gene="ihfA"
                     /locus_tag="ACHKNP_RS13275"
                     /old_locus_tag="pgaptmp_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HGU4704796.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(8231..10618)
                     /gene="pheT"
                     /locus_tag="ACHKNP_RS13280"
                     /old_locus_tag="pgaptmp_002656"
     CDS             complement(8231..10618)
                     /gene="pheT"
                     /locus_tag="ACHKNP_RS13280"
                     /old_locus_tag="pgaptmp_002656"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGU4704797.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(10633..11616)
                     /gene="pheS"
                     /locus_tag="ACHKNP_RS13285"
                     /old_locus_tag="pgaptmp_002657"
     CDS             complement(10633..11616)
                     /gene="pheS"
                     /locus_tag="ACHKNP_RS13285"
                     /old_locus_tag="pgaptmp_002657"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGU4704798.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(11826..11950)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(11900..11944)
                     /gene="pheM"
                     /locus_tag="ACHKNP_RS13290"
                     /old_locus_tag="pgaptmp_002658"
     CDS             complement(11900..11944)
                     /gene="pheM"
                     /locus_tag="ACHKNP_RS13290"
                     /old_locus_tag="pgaptmp_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HGU4704799.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(12067..12423)
                     /gene="rplT"
                     /locus_tag="ACHKNP_RS13295"
                     /old_locus_tag="pgaptmp_002659"
     CDS             complement(12067..12423)
                     /gene="rplT"
                     /locus_tag="ACHKNP_RS13295"
                     /old_locus_tag="pgaptmp_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGU4704800.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(12476..12673)
                     /gene="rpmI"
                     /locus_tag="ACHKNP_RS13300"
                     /old_locus_tag="pgaptmp_002660"
     CDS             complement(12476..12673)
                     /gene="rpmI"
                     /locus_tag="ACHKNP_RS13300"
                     /old_locus_tag="pgaptmp_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGU4704801.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(12770..13312)
                     /gene="infC"
                     /locus_tag="ACHKNP_RS13305"
                     /old_locus_tag="pgaptmp_002661"
     CDS             complement(12770..13312)
                     /gene="infC"
                     /locus_tag="ACHKNP_RS13305"
                     /old_locus_tag="pgaptmp_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGU4704802.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(13316..15244)
                     /gene="thrS"
                     /locus_tag="ACHKNP_RS13310"
                     /old_locus_tag="pgaptmp_002662"
     CDS             complement(13316..15244)
                     /gene="thrS"
                     /locus_tag="ACHKNP_RS13310"
                     /old_locus_tag="pgaptmp_002662"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGU4704803.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            15768..17667
                     /gene="espL1"
                     /locus_tag="ACHKNP_RS13315"
                     /old_locus_tag="pgaptmp_002663"
                     /pseudo
     CDS             15768..17667
                     /gene="espL1"
                     /locus_tag="ACHKNP_RS13315"
                     /old_locus_tag="pgaptmp_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            17839..17946
                     /gene="yniD"
                     /locus_tag="ACHKNP_RS13320"
                     /old_locus_tag="pgaptmp_002664"
     CDS             17839..17946
                     /gene="yniD"
                     /locus_tag="ACHKNP_RS13320"
                     /old_locus_tag="pgaptmp_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HGU4704804.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(17999..18757)
                     /gene="ydiY"
                     /locus_tag="ACHKNP_RS13325"
                     /old_locus_tag="pgaptmp_002665"
     CDS             complement(17999..18757)
                     /gene="ydiY"
                     /locus_tag="ACHKNP_RS13325"
                     /old_locus_tag="pgaptmp_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HGU4704805.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGHQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            19044..19973
                     /gene="pfkB"
                     /locus_tag="ACHKNP_RS13330"
                     /old_locus_tag="pgaptmp_002666"
     CDS             19044..19973
                     /gene="pfkB"
                     /locus_tag="ACHKNP_RS13330"
                     /old_locus_tag="pgaptmp_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HGU4704806.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            20074..20364
                     /gene="ghoS"
                     /locus_tag="ACHKNP_RS13335"
                     /old_locus_tag="pgaptmp_002667"
     CDS             20074..20364
                     /gene="ghoS"
                     /locus_tag="ACHKNP_RS13335"
                     /old_locus_tag="pgaptmp_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU4704807.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            20470..21330
                     /gene="yniA"
                     /locus_tag="ACHKNP_RS13340"
                     /old_locus_tag="pgaptmp_002668"
     CDS             20470..21330
                     /gene="yniA"
                     /locus_tag="ACHKNP_RS13340"
                     /old_locus_tag="pgaptmp_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HGU4704808.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(21371..21907)
                     /gene="yniB"
                     /locus_tag="ACHKNP_RS13345"
                     /old_locus_tag="pgaptmp_002669"
     CDS             complement(21371..21907)
                     /gene="yniB"
                     /locus_tag="ACHKNP_RS13345"
                     /old_locus_tag="pgaptmp_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HGU4704809.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            22054..22722
                     /gene="hxpB"
                     /locus_tag="ACHKNP_RS13350"
                     /old_locus_tag="pgaptmp_002670"
     CDS             22054..22722
                     /gene="hxpB"
                     /locus_tag="ACHKNP_RS13350"
                     /old_locus_tag="pgaptmp_002670"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HGU4704810.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            22885..23475
                     /gene="ydjM"
                     /locus_tag="ACHKNP_RS13355"
                     /old_locus_tag="pgaptmp_002671"
     CDS             22885..23475
                     /gene="ydjM"
                     /locus_tag="ACHKNP_RS13355"
                     /old_locus_tag="pgaptmp_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4704811.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            23608..24999
                     /gene="tcyP"
                     /locus_tag="ACHKNP_RS13360"
                     /old_locus_tag="pgaptmp_002672"
     CDS             23608..24999
                     /gene="tcyP"
                     /locus_tag="ACHKNP_RS13360"
                     /old_locus_tag="pgaptmp_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HGU4704812.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(25003..25379)
                     /gene="ydjO"
                     /locus_tag="ACHKNP_RS13365"
                     /old_locus_tag="pgaptmp_002673"
                     /pseudo
     CDS             complement(25003..25379)
                     /gene="ydjO"
                     /locus_tag="ACHKNP_RS13365"
                     /old_locus_tag="pgaptmp_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
BASE COUNT         6491 a         6469 c         5922 g         6497 t
ORIGIN      
        1 ttttcataaa aaataaaatt gaaggttcat tttataaacc agacattacg tcctacgctt
       61 ctgtgcgttt ttaatttatg ctttcataga attatgtctg catcacggga agaacaaaat
      121 ggataatgct gttgatcgcc acgtttttta tatttctgat ggtacggcaa taactgcgga
      181 ggtattagga cacgcagtaa tgtcacaatt tcccgtcact atcagcagca ttacgctgcc
      241 gtttgtcgaa aatgagagcc gtgcacgggc agtgaaggat cagattgacg caatttatca
      301 ccagacaggt gtgcgcccgc tggtcttcta ctccatcgtg ttgccggaga ttcgcgccat
      361 catcttgcaa agtgaaggct tttgccagga tattgttcag gcgctggttg ccccgctaca
      421 acaagagatg aaactggatc caacgccgat tgctcatcgt acccatggcc ttaaccctaa
      481 taatctcaat aaatatgatg cgcgcattgc ggcgattgat tacaccctcg cccacgatga
      541 cggcatttcg ttgcgcaatc tggaccaggc tcaggtgatc ctgctcggtg tttctcgctg
      601 tggtaaaacc cccaccagtc tgtatctggc aatgcagttt ggtatccgcg cggcaaacta
      661 cccctttatt gccgacgata tggacaatct ggtgctaccc gcgtcgctca aaccgcttca
      721 gcataaattg ttcggcctga ctatcgaccc ggaacgtctg gcggcgattc gcgaggaacg
      781 tcgggagaac agccgctatg cctcgcttcg tcagtgcagg atggaagtcg cggaagtaga
      841 agcgttgtac cgtaaaaatc agatcccgtg gattaacagt accaattatt cggtagaaga
      901 gattgccacc aagctcctcg atatcatggg gcttagccgc cgaatgtact agagaactag
      961 tgcattagct tatttttttg ttatcatgct aaccacccgg cgaggtgtga cacacctcgc
     1021 acttgaaatc agctgcgatt ggtttatcgt gatgcgcatc acttcccggt agtcctgccg
     1081 tagaagcaac aaatttctga gacttgtaat gaacagaact gacgaactcc gtactgcgcg
     1141 tattgagagc ctggtaacgc ccgccgaact cgcgctacgg tatcccgtaa cgcctggcgt
     1201 cgccacccat gtcaccgact cccgccgcag aattgaaaaa atactgaatg gtgaagataa
     1261 gcgactgttg gtcattattg gcccctgctc gatccacgat ctcaccgctg caatggagta
     1321 cgccacccgt ctgcagtcgc tgcgcaacca gtaccagtca cggctggaaa tcgtaatgcg
     1381 cacctatttt gaaaaaccac gaactgttgt cggctggaaa gggctaatct ccgatccgga
     1441 tttaaacggc agctatcggg taaatcacgg tctggagatg gcgcgcaaat tacttttaca
     1501 ggtaaatgag ctgggtgtcc ctaccgcgac tgagttcctc gatatggtga ccggtcagtt
     1561 tattgctgat ttaatcagtt ggggcgcgat tggcgcacgt actaccgaaa gtcagatcca
     1621 ccgtgaaatg gcttcggcac tctcctgtcc ggtaggtttt aaaaatggta ccgatggcaa
     1681 ctcgcggatt gccgtggatg ctatccgcgc agcccgcgcc agccatatgt ttctctcgcc
     1741 agacaaaaat ggtcagatga ccatctatca gaccagcggc aacccgtatg gccacattat
     1801 tatgcgtggc ggcaaaaaac cgaattatca tgccgatgat atcgccgcag cctgcgatac
     1861 gctgcacgag tttgatttac ctgaacatct ggtggtggat ttcagccacg gtaactgcca
     1921 gaagcagcac cgtcgccagt tagaagtttg tgaggatatt tgtcagcaaa tccgcaatgg
     1981 ctctacggcg attgctggaa ttatggcgga aagtttcctg cgcgaaggaa cgcaaaaaat
     2041 cgtcggcggt cagccgctca cttacggtca atccattacc gacccgtgtc tgggctggga
     2101 agataccgaa cgcctggtcg aaaaacttgc ctctgcggta gatacccgct tctgaatgcg
     2161 tgcccattcc tgatgggatg ggcatttctg cgcaacttgt tgtcttctca gcacattact
     2221 gcttgctctg gtcagccata atattgataa taagaatcat tgttatatca attattatta
     2281 atttttatgc gttatacgga tagcagaaaa ctcacgcctg aaacggatgc caatcacaag
     2341 accgcttccc cgcagcctat tcggcgaatt tccagccaga cactgttagg tccggatggc
     2401 aaactgatta tcgatcatga cgggcaagaa tatctgctcc gtaaaactca ggctggcaag
     2461 ctgctgttga ccaagtagcc tttaactcga gcagctgact tccagccgtt taccccagtc
     2521 aggtggacga ctgacatagt catcagctct gtcgctgaaa ggatttcgca acgcctcatg
     2581 caggcggtgc aattccgtca tatcaccctt ttctgccgct tcaatcgccc gttgcgccaa
     2641 ccagttgcgc aacaccagag ctgggttaac gctttgcatc agctgctgac gctcactatc
     2701 gctaacctcg tcttgttgca aacgcccccg ataacgggca aaccagtcat caaatgccgc
     2761 acgatcaata aactcatcac gtagcggtga cgccgcgctg tgctgctcgg tcagactcag
     2821 catgcggaat gtgcgggtat aatcgctgcg ctctcgcgcc atcagactga ataattcatt
     2881 cagtagcgcg ttatcctctt tttgctccgt catgaagccc agtttctgcc gcatccgttg
     2941 tccataatgc gtcaacaaaa cctgctgata gctgtccagg gcctcattca gggcatctac
     3001 ggcaacaaat ggtgacaatg tctgcgccag acgctgtaaa ttccacaacg cgacagcagg
     3061 ttgattatca aagctataac gcccttgatg atccgagtga ttacaaataa aaccgggttc
     3121 gtaatcatcc agaaaaccaa acggcccgta atcaagcgtc agccccagca gcgacatgtt
     3181 atcggtattc atcaccccat gagcaaagcc gaccgtctgc cattgggcaa ttaacgatgc
     3241 ggtacgtgcg acaacatcgc taaaccagag gcggtatttg tcctcatcat cttcaagata
     3301 tgaccagtaa tgacgaatgg caaagtcagc taactgacga accttttccg gctcgcggcg
     3361 atagtaaaaa tgttcgaaat gaccaaagcg cagatgactt ggtgccacac gcatcagcat
     3421 cgcgcctggc tccaccgttt cccgatacac tggcgaatcg ctggtgacga tacttaacgc
     3481 gcgggtcgtc ggaatgccca gataatgcat cgcctcactg gcgagacttt ctcgtatcgt
     3541 cgaacgtaaa accgcccgtc catcacccat tcgcgaataa ggcgtcaggc cagcaccttt
     3601 cagatgccag tccattgtag tgccatcagc aagcagttgt tcgccgagta aaataccgcg
     3661 cccatcaccc agttggcccg cccagacgcc gaactgatga ccactgtaaa cctgcgccag
     3721 tggtgacata ccaggcagta gggtttcgcc gccccagaca cctgcgccat ttttaaacag
     3781 cgacgatgga atactcagcg tgttagcgag ttcggtatta tgccaaatca gccgggcatt
     3841 atttaaaggc gtaggggaaa gtgctgtata ggttgctggc aattcatcgc gccagcgggt
     3901 aataaaagac agggtcatag atcctcctgt ttaatagtgt agacggttac tctcgtctta
     3961 aacaccagca aacaggaggg ttatcgctga accaacgtcg ttatctgatt tataggaacg
     4021 gcaggccaca ggcatccctg gagagcatgg aaatcgaagg gggtaatctg tgccaatatt
     4081 tctgtcgtat caatgccccc cgcgatgatg caattgcaac aaggggaaat ttgcgcctgg
     4141 atcgcacgaa taaacggttc gaaggagcga tgcgtgattt gttgctgaat aaaactttta
     4201 tccagcatga cgcgggtgaa aagaccatca aaaaccgctt tcattgtgct attacctgct
     4261 cccaaattgc ctaacacaag gggatacatt tgcgataagg ataaaagatc gcgattatct
     4321 tttccttcat tgagatgcgg gtaattctca ttaatcaaca gttcaataaa cggatatttt
     4381 aatagttcgc ctgcgtaatt atccctctct aataataatg tagccacttg tggcgttaaa
     4441 ttaagccagg caaagagttt atgttgaata aaaaaatgtt ggcatgattt tagtaattcc
     4501 aactgctctg aaaataattg ccaatgttgc tcctcggtaa gttgcgctat aacccgactc
     4561 gtcgggatac gaaccgtacc atcttcactg gagaaatggg taattaactc aacgccgaca
     4621 aggtcttgct ggttatctct tatcggcagg aaatagcaat cagaatgata aagattctcc
     4681 aaaaaaatct tcatggtaac cgtccctctc gaaggatgtt tttcagtatc cggctaaaag
     4741 ggatgaggcc ataagacata acaattacat aaggagtaac tttcatttgt tccatgttaa
     4801 ccactttttc aggggtcctt tttttagatt atcctgatta taaacgaata atccatttac
     4861 ggaatttttg tctgcaaaat actactgtat tcaggagtaa atggacggcg atgtatcatc
     4921 gccgtccatt tttcgacata ctaaattcgt ctcgcctgcc agaaattttt ctgccaatag
     4981 acattatcaa gtgaggaacg catcactccc ttgctggtag aggcgtggat aaattggttg
     5041 ttggtatcgt aaatacctac atgcaaacca ttttgtccgg aacccgtttt gaaaaagacc
     5101 aggtcaccag gcagcaactc gtctttatca atttgcgtgc cgatagaggc ttgttctttg
     5161 gtttctcggg gcagctgcaa atcgaaacga tcgcgcatcg ttacaaccac aaatcccgaa
     5221 cagtccacac cgcgccgcgt catgccacca taacgatacg gcgtgccatg ccagctttgt
     5281 agctggtcgt tcaaaccggc aataacggta atcgaatcag aaagtctggc atttggcggc
     5341 ggtgctttat ggtagctaca cccggccaga aacagtgctg tgatcaaaat aaggcagaaa
     5401 cgcattccgt acggttcctc tgttttttat tcttgcatta atttagcgtc gtaattaccc
     5461 gattttcaag atactaatga aatcagatgg tcgaaatcag cattctgtga ccttcgatat
     5521 ccagacggcg aaaattcatc ccataggcct gcgccagatt tggcggcgtg agcacctctt
     5581 ctctgcgtcc actggccagc atttttccac cttttagcaa ccacgcccga tgcgcatgac
     5641 gcaatgtgtg gttgagatcg tgactgctca tcacaatcgc cagtccttgc tgacacagag
     5701 cgctcagaat tttgtctaac gcactttgtt gcgcaacatc aagactgttc atcggctcat
     5761 caagaagcag caattgacct gcgggattgg cttgtggtgt gatttgcagc accaccgcag
     5821 caagacgtac gcgttgccat tcaccgccgg aaagttgatt ggtgctacgt ccgagtttgt
     5881 catcaagagc cagcgcccct gcgacatcat tcagtagttc ggtacgcgtt ttatcgtgct
     5941 gatgcagtgt caggtagtgc cagaccggca ttgcaaacgg cggcgtctgc tgttgtgaaa
     6001 ggtaggcgcg atgcagcgcg agttttgttg cggaccatgc ttccagtggt tgccccgcga
     6061 actgaatgct tcccttaccg ctggtcattc cggccattcg tgcaagcaag gtactcttac
     6121 ccgcgccatt cggccccacc aggtgcagga tctccccagc ccgaacctcg ccagaaagcg
     6181 gccccaggcg ggtagtttcc gcaacatcct gtaactgcat cacaatagac attattttgc
     6241 caacgccagt ttaatgcttt ccatcacaat gggatcttca ggcgtcatat ccggggaaaa
     6301 acgctggatg accaacccgt ccctgccaac taaaaatttc tcaaaattcc ataaaatatc
     6361 atcagggtac agcggtgcac gacctttgct gaccatacgg gcatagaatc cgctctcttc
     6421 cggcgcgact gcggtcggcg ctgcggcaat caatttttga tacagcggat ggcgtccttc
     6481 gccattaact tcaatcttac tgaacatcgg gaacgtcacc ccccatgtgg tggtacagta
     6541 agttttaatc tcttcatcgc tgcccggttc ttgttccaga aactggttgc acgggaatcc
     6601 cagcaccaca aaacctcgat cggcccaggc tttctgaata ttctccaact gctcatattg
     6661 cggcgttaag ccacactttg aggcgacatt gacaatcaac agcacattac cagcgtactt
     6721 ctccagcgtg gtcacttcac cgtcgatatc tttcactacg gtcgtcagaa tggaatcttg
     6781 catcgtttct cctgggtgtg gtcagtaaaa atcttagctt ttaatcatag accgtctttt
     6841 tgcggctaac gtcttgcttt taacaataac cagataaaca ccggcgcacc caacgttgcg
     6901 gtgaccacgc caataggcag ctctgcggca gctaatgcca ggcgcgctac aatatcggcc
     6961 agcagcaatg cgctcgcccc tgccagcgcg cagccgggaa gtaatacgcg atgatcggtt
     7021 aaaccacaca accgaagaat atgtgggatc accagaccaa taaagccgat agcacccgcc
     7081 agcgccacac tgacgccaac catccagccg gtcgcggcca ccagcacatt gcgccagaac
     7141 cacaggggta aacccagttg ccgcgccgag atctcgccaa gtgctaacat attcatcggc
     7201 ctggactgac aacagatcca caacaacacg gggatcaatg ccagcatcag ccagctttgc
     7261 cgccagtcta cgccgccaaa accgcccatc atccagtaca tcagctgacg taaatcaaca
     7321 gaggtggaaa agtagatagc ccacgtcatt agtgcgctac agataatccc taatgcaacg
     7381 ccagccagca ataaccgact ggtcgaaaga tgacgacggg cgaaacgtaa gagtattaaa
     7441 gtgatgataa gcgcgccagc aatcgcacac agccctagcg cccagttggg gagttgccct
     7501 tgcccaagca ataccgcggc gataagcccc acgcctgcgc cgttagagac gccaagtagt
     7561 ccaggttctg ccagaggatt ttcaaacaac gcctgcatta cagcgccgga tatagccagc
     7621 gccgcaccaa ccagcaatac agccagcgta cgtggcaggc gaatttgcca gacgaacagt
     7681 tcgccacgag gagtaaacca gtcacctggc gagatccatt gttcaccggc gcaaaggctt
     7741 aagagaagcg ccagcagcat caaaactgac aggcataata accagcgaat attttgtcgc
     7801 tgttgttggc gggcaagtgt cagcatggta tccgttctgc tgaagtgtca tggcgttgat
     7861 tttacggtga ctcttcgaca gtgaaaagaa aaaaggccgc agagcggcct ttttagttag
     7921 atcagattac tcgtctttgg gcgaagcgtt ttcgacccgg ctttttaact tctgcccggg
     7981 tctgaaggtc accacgcgcc gtgctgtaat gggaatatcc tcgcccgttt tcgggttacg
     8041 tcccgggcgt tgattcttat cacgcagatc gaagttacca aaaccagaga gtttcacctg
     8101 ttcgccgttt tccagagcgc gacggatctc ttcgaaaaac agttcaacca gttctttggc
     8161 atcccgcttg ctaagcccaa gcttatcaaa cagatattct gacatttcag cttttgtaag
     8221 cgccataggt tcaatccctc aatgatgcct ggaatcgctc ttttaatgcc tctacacatt
     8281 tggcgacggt agcggcaatc tcctcttctt cgagtgtacg gctggtatct tgcaggatca
     8341 ggcttatggc gaggctctta tacccctccg caacaccctt accgcggtac acgtcaaata
     8401 agtttacgcc aactacctga tttacgccaa ctttcttaca ttcggataaa atatccgctg
     8461 cgggaacgtt ttctgcgacc accaccgcga tgtcacgacg gttcgccggg aagcgagaaa
     8521 tctcgcgcgc ctgaggcacc acgcggtctg cgagcttgtt ccactccagt tcgaacacca
     8581 gagtgcgacc gttaagatcc agtttacgtt ccagttcagg atgaacaacc ccaacaaaac
     8641 caatacgttc acctttcaga taaatcgctg cggattgccc cggatgcagt gccggattcg
     8701 cttctgcacg gaactcaacc tcattcagtt taccggtcag gtcgagaacg gattcaagat
     8761 cgcctttcaa atcatagaaa tcaacggtct cttttgccag gttccagtgc tcttcgtaac
     8821 ggttaccgca aatcacaccg gctaacatca gatcctgacg aatgcccaac ggtgcctgag
     8881 tatctggtac gaaacgcaga ccgctttcga aaatgcgcac acggttctgc tgacggttct
     8941 ggttgtacac cacggttgcc agcaggccgg tccacagaga aagacgcatt gctgacattt
     9001 caacagagat cgggcttggc agcagtaagg cttcaacgcc tggatggatc atctgctgca
     9061 ctttcggatc aacgaagctg taggtgatca cttcctgata gcctttgtcg ttgagcagcg
     9121 ttttcacgcg cttgagcgac aggtcagctt cacggtgagt acccataatc aggcttgcct
     9181 gtaccggctc atccgggatg ttgttgtagc cgtaaacacg cgcgacttct tcgaccagat
     9241 cttcttcaat ctccatatcg aaacgccagc tcggcgcaac tgcctgccac tcgtctttgc
     9301 cttcggtcac ttcgcagccg agacgacgca gaatgtcagt tacctgctca tccgcaatat
     9361 gatggccgat caggcgatcc agtttgctac gacgtaaagt gatggttgca cgcttcggca
     9421 gcgttgcttc gttggtgata tcaattaccg gaccagcctc accaccgcag atgtcgatca
     9481 gcagacgggt cgcacgttcc atcgctttgt gctgcagtgc cggatcaacg ccacgctcat
     9541 aacggtgaga cgcatcagta tgcaggccat gacgacgagc acgaccggtg atagacagcg
     9601 ggctgaagaa agcgcattcc agcaagacgt tttgcgtttc gtcattcacg ccagagtgtt
     9661 cgccaccaaa gatgccgccc atcgccagcg ccttgttgtg gtcggcgatg accagagtgt
     9721 cagcattcag cttcgcttca gtgccatcaa gcagtaccaa cgtttcgccc tctttcgcca
     9781 tccgcaccac aatgccgcct tcaatgcgat ctttatcgaa agcgtgcatc ggctggccca
     9841 gttcgagcag tacatagttg gtgacgtcaa caactgcatc gatagaacgg atcccgcaac
     9901 gacgcagttt ttctttcatc cacagcggag ttggcgcttt aacgttaatg ccttttacca
     9961 cacggccaag ataacgcggg caggcttccg gcgcttcgac tgtaatcggc agcgtgtcgt
    10021 cgatggtcgc accaaccgga acgatttccg gttcaaccag cggcagctga ttcagcacgg
    10081 caacgtcacg cgcaacacca atgataccta aacagtcggc acggtttggc gtcacgctga
    10141 tttcgatggt gttgtcatca agtttcaggt attcacggat gtcggtgcca atcggcgcat
    10201 ccgcaggcag ctcgataatg ccgctatgat cgtcagaaat tcccagctca gaaaaggagc
    10261 acagcatccc ttcagacggt tcaccacgca gtttcgccgc tttaattttg aaatcacccg
    10321 gcagaacagc accaatggta gctaccgcca cacgcaggcc ctgacggcag tttggcgcac
    10381 cgcagacgat gtccagcagg cgatcgccgc caacattcac ttttgtcaca cgcagtttgt
    10441 cagcgttcgg atgctgcgca cactcaacca cttcaccaac gaccacgccg tggaagctac
    10501 cggcaaccgg ttctacaccg tcaacttcca ggcccgccat agtgatttga ttcgccagcg
    10561 catcgctatc aatcgccggg ttcacccatt cgcgtaacca cagttcactg aatttcataa
    10621 tctattcctg ccttatttaa actgtttgag gaaacgcaga tcattttcga agaatgaacg
    10681 caggtcggtg acgccgtaac gcaacatagt cagacgctcc atccccatcc cgaaggcgaa
    10741 accagagtaa acttccgggt cgatgccaac gttacgcagc acgttcggat gcaccatccc
    10801 gcaacccagc acttccagcc atttaccgtt tttacccatg acgtcaactt ctgcagaagg
    10861 ctcggtgaac gggaagtagg aaggacggaa gcgaatctgc aaatcttcct caaagaagtt
    10921 acgcaggaaa tcgtgcagag tgcctttcag gttggtaaag ctgatgttgg tatcaacaat
    10981 cagaccttcc atctgatgga acatcggcgt gtgagtctgg tcgtagtcgt tacgataaac
    11041 gcggccaggc gcaatgatac gaatcggtgg ctgctgggct ttcatggtgc ggatctgtac
    11101 gccagaggtc tgggtacgca gcaggcgggt agtgtcaaac cagaaagtgt cgtggtcagc
    11161 gcgcgccggg tggtgaccag gaatgttcag agcatcgaag ttatgataat cgtcttcgat
    11221 ttccggcccg gttgccacgg taaagccaag ctcaccgaag aaactttcga tacggtcgat
    11281 agtacgggta accggatgca gaccgccgtt ttcaatgcga cgacctggca gagagacatc
    11341 aatcgtttcc gccgccagac gcgcattcag tgcagcgctt tccagttccg ctttacgcgc
    11401 attcagcgcc tgctgaacct gctctttcgc ttcgttgata accgcaccag ctgccggacg
    11461 ctcttctggc ggcagctcac gcagggtcgt catctgaagg gttaagtgcc cttttttacc
    11521 caaatattcg acgcgcacat tatctaacgc ggcaacatct gacgcctggc taatggccgc
    11581 cttcgcactg gcaaccagtt ctgcgagatg tgacatggtt ttcctcattg tgtcagtggt
    11641 gacactggtt cgttggactt agagcctatc ccatcaggct attttacttg ccattttggt
    11701 cccgggcagt gctcgaaatt ctcacgtact taaatacgct ccggtttctc cgcgctggcc
    11761 gtgtccagac tggctgcgac aattacacct gatgagacag gctttttatt tttcaaaacg
    11821 cgcatacaaa aaaagcctcc actgggaggc tttcaggcgc tgttttccgt ttctcttctc
    11881 acgcgctagc ctcctggatt caggtgctaa agtaaaaaaa gaagcggaaa atagcagcat
    11941 tcattgcttg cgttaccttt tggtactctt caaaagacct ttattgaaaa ggctacggcg
    12001 ataaaagtca atgttttgat ggcgttgaaa cgaaaagagg gagactagct ccctctttca
    12061 actggcttat gccagagctg ctttcgcttt ttcaaccaga gcggtgaacg ctactttgtc
    12121 gaatactgcg atatcagcca ggatcttacg gtcgatttca acagaggctt ttttcaggcc
    12181 attgatgaat ttgctgtaag aaataccgtt ctgacgtgct gctgcgttga tacgcgcaat
    12241 ccacagttga cggaactgac gcttacgttg acgacggtca cggtaagcat actgaccagc
    12301 tttgataaca gcctggaagg caacgcggta tacgcgagaa cgcgcaccgt agtagccttt
    12361 agcttgtttc aaaattttct tgtgacgtgc gcgtgcaata acaccacgtt ttacgcgagc
    12421 catatgtgct ctcctgtatc tatattctaa ttaaaaagtt aaaaacgtta acggcttatg
    12481 cgtacggcag gcacgcgatt accaggccca gatcgccttt ggaaaccatg gctttcggac
    12541 gcaggtgacg tttacgtttg gtcgcttttt tggtcagaat gtgacgcagg ttagcgtgct
    12601 tgtgcttaaa accaccttta ccggtttttt tgaagcgctt agcagcaccg cgtacggtct
    12661 taatttttgg cattttaata acttccactt cgcattgtta ataaacgaaa caaaggcgaa
    12721 caaagcctgt gaagcccgaa ggctccacag acagtgctac ttgaaggcct tactgtttct
    12781 tcttaggagc gagcaccatg atcatctgac ggccttcgat cttcgttggg aaggattcga
    12841 ccactgccag ttcttgcaaa tcgtctttca cgcgattaag cacttccata ccgatttgct
    12901 ggtgcgccat ctcacgaccg cggaaacgca gcgtgatttt ggctttatca ccctcttcga
    12961 gaaagcgaat caggctgcgg agttttacct gatagtcgcc ttcatctgta ccaggacgga
    13021 atttaatttc cttaacctgg ataacttttt gctttttctt ctgttcctta gaagacttgc
    13081 tcttttcata gaggaatttg ccgtaatcca ttatacgaca aaccggcggc tcggcgttag
    13141 ggctgatctc gactaagtct actccggctt cttctgcttt ctccagagct tctctcagac
    13201 tcacaatacc aagctgctcg ccttccagac ctgttaagcg aacttcctgg gcgcgaattt
    13261 cgccattgat acggttaggg cgcgccgttt gaactcgttt tccgccttta ataccttatt
    13321 cctccaattg tttaagactg cggctgcgaa tctcttgttg cagcttctcg atcacttcat
    13381 ttacgtccat gcttcccagg tctttaccac ggcgggtgcg aacggcaact ttgcctgatt
    13441 ccacctcttt atcaccacag accagcatat atgggacgcg acgcaaagtg tgctcgcgga
    13501 ttttaaagcc aatcttctca tttctcaagt ctgctttaac acgaatgccc gcatttgata
    13561 gtttttgcgt caattcgtta acgtattcag actgtgaatc ggtaatattc atgataacaa
    13621 cctgaactgg cgcaagccag gtcgggaaga aaccagcgaa ttcttcggtc aggataccga
    13681 tgaaacgttc catcgatccc agaattgcgc ggtgaatcat taccggtact ttacgttcat
    13741 tgtcttcgcc tacataagaa gcgctcagac gagaaggcaa agagaagtcc agctgtactg
    13801 taccgcactg ccatgcacga tcgaggcagt catacagggt aaattcaatt ttcggaccgt
    13861 agaaagcgcc ttcacccagt tgatattcaa acgggatgtt gttttcttcc agcgcaaccg
    13921 ccaggtccgc ctcagcacga tcccacattt catcactgcc aatacgtttt tcaggacgag
    13981 tggagagttt gacgacgatc ttctcgaagc caaaagtgct gtacatatca tagactaaac
    14041 ggatacatcc gttaacttca tcgcgaattt gttcttcagt acagaagata tgcgcgtcat
    14101 cctgggtaaa tccacgcacg cgcatcaggc catgcagcga acctgacggc tcattacggt
    14161 ggcagctacc aaactcggcc atacgcagcg gcagatcgcg ataagacttc agcccctggt
    14221 tgaaaatttg tacgtgaccc gggcagttca tcggcttaat gcagtattca cggttctcag
    14281 aagacgtggt gaacattgca tctttgtagt tgtcccagtg accggttttt tcccacagga
    14341 cacggtccat catgaacgga cctttaactt cctgatactg gtactctttc agtttagaac
    14401 gaacaaacac ttctagttca cggaagatgg tccagccgtc gttgtgccag aataccatac
    14461 ccggcgcttc ttcctgcata tggtacaggt cgagctgttt accgatttta cggtggtcgc
    14521 gtttcgcggc ttcttccagg cgctgcaggt aagcgttaag cgcttttttg tctgcccacg
    14581 ccgtaccgta aatacgttgc aacattttgt tgttgctgtc gccacgccag taagcccctg
    14641 ccgttttcat tagtttgaaa tgatggcaga aacgcatgtt cggtacgtgc ggaccgcggc
    14701 acatatcgac atattcttca tggaagtaca gacctggctt gtcatcatgg gcgatgtttt
    14761 cgtcaagaat ggagactttg tagctctccc cacggttggc gaaagtttca cgcgcttcgt
    14821 gccagctgac tttcttctta atgacgtcgt agtttttctc agcaagctca tgcatccgct
    14881 tctcgagtgc ttcgacatct tcctgggtta acgtgcggtc aagatcaacg tcgtaataaa
    14941 aaccgttgtc aataaccggg ccgattgcca ttttggtatg cggccaaagt tgtttaatcg
    15001 cgtgccctaa caggtgcgca caggagtgac gaatgatctc cagaccgtct tcgtctttgg
    15061 cggtaatgat ggacagttgt gcgtcgtttt caatcagatc gcaagcatca accagttcgc
    15121 cattaacgcg ccctgcgata caggctttcg ccagacctgg accaatgtcc agcgcaacat
    15181 ccatggggct tacagcgtgg tcgtaatggc gttggctgcc atcaggaaga gttataacag
    15241 gcattttata tccttatttg cagtggtgac ccacacgaaa gatcacatac aaagaaaaat
    15301 ttgtttatta acagttcatt gcgaaaccat ctagccaaca aatgctaaat tggttcgcaa
    15361 tcaggtacac aaatgaaaga cattaacctc tcaccttcca cccggttgag cgatgttaac
    15421 actaaaaaaa gggagattgt acctttccgt ttcacattga ttcgattcgc aattcgtttg
    15481 tagtgagtac accagcaaaa cgcttaaaga accactgata acatcacggt gcgtctgaaa
    15541 ggtttactgc gagcggattt acagcagcat aaactgaatc cagcaaagtt cgttgcgtga
    15601 tttggcggta gtattttaaa cgctcatgag attaatgacg aagtggtcat atcacaatga
    15661 taaaagtgac acaattctta taacaatttt tcgtgcacat ttcgttctgg cgataataat
    15721 taatcatcat cctcaaaacc tccagatata tataaggcga atagattatg agtcaaaacg
    15781 atatcattat cagaactcat tataagtctc ctcatagatt gcacatcgat agcgacatac
    15841 caacaccttc atcagagcct attaataaat ttgcgcgcca gctcatcacc ctacttgata
    15901 cctctgactt aagttcgatg ctgtcatact gtgttactca ggaatttacc gcaaactgtc
    15961 gaaaaatatc acaaaattgt tattccactg ccctttttac cattaacctt gccacttcac
    16021 ccatccatgc agaaaatata ctcattacat tacactataa aaaagatatc atttccttat
    16081 tactggaaac cacgcctatt aaagctaacc atttgcgaag catactggat tatattgaac
    16141 aggaacagtt aactgctgaa gatcgtaacc attgtatgaa actgtctaaa aaaatccata
    16201 gagaaaaaac tatacaccca acagtaaatc tcaatggtag tgcatgtttt ttgcaatctc
    16261 cttctgacgc tattttttgt cgccatctgt cattgcaata cgcccttgat tcattgagaa
    16321 atggaaaagg caaagtcaac ctgattaaac attactcctc cgttgaatcc atacagcagc
    16381 atgtcccctt agtccgggac gcggagttca gagcattact tcgccatcct cctgcaggga
    16441 gtcgcgttat cgcgagtaag gattttggct tcgctttaga ttttttcttt tgtcgaatga
    16501 tggcaaacaa tatcagtcat atgtccgcga ttttatatat agacaatcat actttgtcag
    16561 taaggctacg aataaagcag tcagtgtatg gacaattaaa ctatgttgtg tccgtttacg
    16621 acccgaacga taccaacgtt gccgtcagag acacccacag gacagcacgg ggctttcttt
    16681 cgcttgataa gttcatcagt tcaggtcccg atgctcagac ctgggctgat aggtatgttc
    16741 gcaactgtgc aattgctatt ctgcccctat tacctgtggg agttccaggg gctattttcg
    16801 cgggtattgc atcacgaatg ccatttgccc ctatacatcc atcggcaatg ttgttaataa
    16861 tggctacagg ccagactcaa cagcttatta cattattcaa acagttgccc atactccctg
    16921 aaaaagaaat cattgaaata ataactgcgc agaatagcgt tggtacacct gctttatttt
    16981 tggctatgat gaacggacat actgacaacg tgaaaatatt tatgcaagaa attcagtcac
    17041 tggtagataa tcacatcatt catgaagata acctggttaa attactgcaa actaaaagtg
    17101 ctaacgaaac acctggactc tatatctcca tgttgtatgg attcgatgaa ataatcgata
    17161 tctttctgaa tgcattaacc actccaatag cacaagaact tttaaacaaa aaaatggtga
    17221 tggatatttt agccatgaaa acacgtgatg gtgaaccagg gttatttgct gcaatggaaa
    17281 ataatcaccc tttgtgtgtc acacggttcc tctctaaagt ttatgggatc gccgttaaat
    17341 ataaactcag caaaatcaac atcatggact tattaaaagg cgcgacagca cagggaaccc
    17401 ctgcattata catcgccatg agcaagggta atgaagacgt cgtgttatct tatatatcga
    17461 cgctgggtgc ttttgcaaaa aaaacattct tttagtcaac atcagttatt tacactattg
    17521 gccgctaaaa atcatgacaa catgtcagct gttcatatag ccattcatca taagcattat
    17581 aaaactgtag aaacatatta tgctgctata aatgtcatca gccaaagcct gagttttagt
    17641 gctgatgaaa taaagacgta tttataacca gctatttacg atatatatcg atacataatt
    17701 ttattttttt cataaaacgc agaaacagtg accaggtgta caacgccacc caacggctgt
    17761 aataacaaaa aatcccttga tgcccgtccc ttttgttaca ctccgttatc acgcacaaga
    17821 gatatgcagg acactggtat gccgactaaa cgctttgata aaaaacactg gaagatggtg
    17881 gtggtgctac tggcaatctg tggcgctatg ttgttgctac gttgggcagc aatgatttgg
    17941 ggctgagcag tgacaaacga agacagccag accgcataac acggtccggc agataatttt
    18001 acatgctgta gcccagcgat aaggttgtcc gacggtcggt atgttccggt gcagactctg
    18061 gaggttcaga gttccatgtg acgttgtaag ccaccttcag tccaaaatgt tcattaatag
    18121 caacatttaa tgcgctttcg gagttcagcg ttgtgtcttc cgcgccaaag accgaaacac
    18181 cctgcgtaaa tttagcgttg tcagtcaact gccatgcata agccccggag gcatagccca
    18241 gcggctgggt ttcactggca ttgtcggtat atttgtcgta acgcacacct ggaccgaatt
    18301 caaagcggaa actgtgtacc gggccattca aaaactgatg accataaccc gcggtcaaca
    18361 catcgcgctc tcgatagccg ttataacggt ctgtcagcca gcttgcctga ccaaataaat
    18421 agtcataatc agttaaatta aaacggctac gcccgcccgc cgcatatttt tctgaagaac
    18481 gctcatcatt agaagaagta ttactggcgt tcccccacag cgaccaggca gtggtttgtc
    18541 cataccaggt catggtggtg tcagccgtaa gtgaggagct tttcgtattg cctgattgtg
    18601 caagatatcc tgcgttcaga ttaccttcga aaggtttttt agcgctggca ggatcgtcca
    18661 tgacagtaaa aacggaatca tcggcagctg cattcagtga cgcaaacatg ccccccgcca
    18721 acataacgat ggcaggaact gtcttcaaaa gcttcattta tcaagagtcc gtacaacaaa
    18781 aaaagagacc atcgcggtcc cggaaacttt cttaaggatc aaagattagc gtccctggaa
    18841 aggtaacgaa ttataaaaag gcgcgaataa cttagcaatg tattcttatt tcattttttg
    18901 aataagcatg tggcgaaaac agatttttat ttatatatat ttatctgcaa aattttaaat
    18961 aaagctctaa taaatcatat tgttaatttc ttcactttcg gctgattcgg tgccagactg
    19021 aaatcagcct ataggaggaa atgatggtac gtatctatac gttgacactt gcgccctctc
    19081 tcgatagcgc aacaattacc ccgcaaattt atcccgaagg aaaactgcgc tgtaccgcac
    19141 cggtgttcga acccgggggc ggcggcatca acgtcgcccg cgccattgcc catcttggag
    19201 gcagtgccac agcgatcttc ccggcgggtg gcgcgaccgg cgaacacctg gtttctctgt
    19261 tggcggatga aaatgtcccc gtcgctactg tagaagccaa agactggacc cggcagaatt
    19321 tacacgtaca tgtggaagca agcggtgagc agtatcgttt tgttatgcca ggcgcggcat
    19381 taaatgaaga tgagtttcgc cagcttgaag agcaagttct ggaaattgaa tccggggcca
    19441 tcctggtcat aagcggaagc ctgccgccag gtgtgaagct ggaaaaatta acccaactga
    19501 tttccgctgc gcaaaaccaa gggatccgct gcatcatcga cagttctggc gaagcgttaa
    19561 gtgcagcact ggcgattggt aacatcgagt tggttaagcc taaccaaaaa gaactcagtg
    19621 cgctggtgaa tcgcgaactc acccagccag acgatgtccg caaagccgcg caggaaatcg
    19681 ttaatagcgg caaggccaaa cgggttgtcg tttcccttgg tccacaagga gcgctgggtg
    19741 ttgatagtga aaactgcatt caggtggtgc caccaccggt gaaaagccag agtaccgttg
    19801 gcgctggtga cagcatggtc ggcgcgatga cactgaaact ggcagaaaat gcctctcttg
    19861 aagagatggt ccgttttggc gtagctgcgg ggagtgcagc cacactcaat cagggaacac
    19921 gtctgtgctc ccatgacgat acgcaaaaaa tttacgctta cctttcccgc taacaaaaac
    19981 attcccccag cattggggga atcaccacca acctgtcggc aacgcgtttc tccgactatg
    20041 ctcaaaagtc atgtgataac aaaggggtga actatggcca gtggcgatct tgtccgttat
    20101 gtcataaccg tcatgttgca tgaggataca ttgactgaaa ttaacgagtt gaataattac
    20161 ctgactcgcg acggtttttt gctcaccatg acggatgatg agggaaatat ccatgagctg
    20221 gggactaaca cttttggact tatcagtacc caaagtgaag aagaaattag agaactggtt
    20281 tcggggctta cccaaagtgc aaccggcaaa gatcctgaaa tcaccatcac gacctgggag
    20341 gaatggaata gcaacagaaa ataaatggtt tttgggcaat aatcagtctg tggtgtgcgt
    20401 tagctcgtgt ttttacaccg cattcttgcg ctaaccttat gatctggcag acaacatggg
    20461 agagacatca tgtggcaggc aatcagtcgt cttttgagcg agcagttagg tgaaggcgaa
    20521 atcgaactgc gtaatgaact gcctggcgga gaagtccatg ccgcatggca tttgcgctat
    20581 gcaggacatg actttttcgt caaatgtgat gaaagggaac tgcttcccgg ttttaccgcc
    20641 gaagccgacc aactggagtt actgtcgcgt agtaaaaccg tcaccgtgcc taaggtttgg
    20701 gcagtaggcg ctgaccgtga ctacagtttt ctggtgatgg attatctccc acctcgtccg
    20761 ctggatgcgc atagcgcatt tattcttggt cagcaaattg cgcgtttaca tcaatggagt
    20821 gaccaaccac aatttggcct cgattttgat aactcgctct ccacaactcc acagcccaac
    20881 acctggcaac gtcgctggtc aacgtttttt gctgaacaac ggattggctg gcagttggaa
    20941 ctggcagcag agaaagggat cgctttcggc aatatcgacg ccatcgtcga gcatattcag
    21001 cagcgtctgg cctcgcatca accgcagcct tctctgttgc acggcgattt atggtccggc
    21061 aactgtgcgc tgggtccgga tggcccgtac attttcgacc ctgcctgcta ctggggtgac
    21121 cgagagtgcg acctggcgat gttaccgctg catactgaac agccgccaca aatctatgac
    21181 ggctatcagt cagtatcccc gctacctgcc gatttccttg aacgtcaacc ggtttaccaa
    21241 ctctacacgc tgttaaatcg tgcaagatta tttggcggtc agcatttggt tattgctcag
    21301 cagtcattgg atagattatt agcagcatga tatgggttga ggataatggc cgctccgtgc
    21361 ggccttttga ttaaataaac ccaagcagag aaaagaacac ataacctgca gcaatacaaa
    21421 taaccggcag gatatatagt gagaaaaact gcaggaaaat agtatggtgc ggaacaacaa
    21481 tacgggactc aatttgttca cgcgttagcc cctcttcccc tttggctttt tccagaatga
    21541 gttgatcttc aacaccttcc cgcaagaagc gcgcctggcg actcatccgg gcaccggaat
    21601 cctgcaacgc cagtccgaca aaaatcagga tgaaaatcac ccagaacata acgttcacgc
    21661 caccattaaa attgggcgtc ggggagttat accagaacag attcaaaaaa ggcgtattcg
    21721 cctgcatcat atcgatcatg acatgagtaa agtcgagcat gaccgcatta atgccttcct
    21781 ggttttcctg ccgggtattc ataaatttca gcaaagaaat caatgtagag atcaatgcag
    21841 gtataaaaat cacccaaccc aaaatccttt tcaaaacagc aatgcgtcca gcttgttgat
    21901 acgtcatgag ttctccttga ttaagacgcg tcgtttcact tagtttacct gtagatatct
    21961 gttttcgccc attctttaaa ggcgatatga taggcgctta atcataagca cggcttaata
    22021 ccttacacat aatgctctaa aggagaggtt gtaatgtcaa ccccgcgtca gattcttgct
    22081 gcaatttttg atatggatgg attacttatc gactcagaac ctttatggga tcgagccgaa
    22141 ctggatgtga tggcaagcct gggggtggat atctcccgtc gtaacgagct gccggacacc
    22201 ttaggtttac gcatcgatat ggtggtcgat ctttggtacg cccggcaacc gtggaatggg
    22261 ccaagccgtc aggaagtagt agaacgggtt attgcccgtg ccatttcact ggttgaagag
    22321 acacgtccat tattaccagg cgtgcgcgaa gccgttgcgt tatgcaaaga acaaggttta
    22381 ttggtgggac tggcctccgc gtcaccacta catatgctgg aaaaagtgtt gaccatgttt
    22441 gacttacgcg acagtttcga tgccctcgcc tcggctgaaa aactgcctta cagcaagccg
    22501 catccgcaag tatatctcga ctgcgcagca aaactgggcg ttgacccact gacctgcgta
    22561 gcgctggaag attcagtaaa tggcatgatc gcctctaaag cagcccgcat gcgttccatt
    22621 gtcgttcctg cgccagaagc gcaaaatgat ccacgttttg tattagcaaa cgtcaaactc
    22681 tcatcgctga cagaactcac cgcaaaagac cttctcggtt aatgaccagg ggcagtgatc
    22741 tcgctgcccc tggttcttta tctgaatttg cattcaactg acggattaat cgtcaattta
    22801 agagaaagag ttacaccgtc accacttccg tgcactgtat aaaaatccta tactgtacgt
    22861 atcgacagtt tagtgagttt tatcatgacg gcggaaggtc accttctctt ttctattgct
    22921 tgtgcggtat ttgccaaaaa tgccgagctg acgcccgtgc tggcacaggg tgactggtgg
    22981 catattgtcc cttccgcaat cctgacgtgt ttgttaccgg acatcgatca cccaaagtcg
    23041 tttcttgggc agcgattaaa gtggatatca aaaccgatcg cccgcgcttt tgggcatcgt
    23101 ggttttaccc acagtctgct ggcggtattt gcgctgctgg caacctttta ccttaaggtt
    23161 cctgaaagct ggttcattcc ggctgatgcg ctacaaggaa tggtgctggg ttatttaagc
    23221 cacatacttg ccgatatgct gacacccgcc ggtgttcccc tgctctggcc atgtcgctgg
    23281 cgtttccgct tgcctatcct ggttccccaa aagggcaacc aactggaacg ttttatctgc
    23341 atggcattat ttgtctggtc ggtatggatg ccccattcat tacccgaaaa cagcgcggtt
    23401 cgttggtcat cgcaaatgat caataccttg cagatccagt ttcatcggct tattaagcat
    23461 caggttgaat actaaaaagg caaaaatcac ctttctggaa taagcaattc catttgaata
    23521 taagagccag ctcacagttc tgttaatctt gcgccaacac tatgactgct acgcagtgat
    23581 agaaataata agatcaggag aacggggatg aactttccat taattgcgaa catcgtggtg
    23641 ttcgttgtac tgctgtttgc gctggctcag acccgccaca aacagtggag tctggcgaaa
    23701 aaagtgctgg tgggtctggt gatgggtgtg gtttttggcc ttgccctgca taccatttat
    23761 ggttctgaca gccaggtact taaagattct gtacagtggt ttaacatcgt tggtaacggc
    23821 tatgttcaac tgctgcaaat gatcgttatg ccgttagtct tcgcctctat tctgagcgcg
    23881 gttgcccgtc tgcataacgc atctcagtta ggcaaaatca gttttctgac catcggtacg
    23941 cttttgttta ccacactgat tgcggcgctg gtcggtgtgc tggtcaccaa cctgtttggt
    24001 ttgacggctg aaggtctggt tcagggaggt gcagaaactg cacgtctgaa cgccatcgaa
    24061 agtaactatg ttggtaaagt ctctgacctg agcgttccgc agctggtctt gtcctttatc
    24121 ccgaaaaacc cgtttgccga tctgaccgga gccaatccga cgtcaattat cagcgtggta
    24181 atttttgccg cattcctcgg tgtagctgcg ctgaaactgc tgaaggatga tgcgccgaaa
    24241 ggtgaacgcg tcttaaccgc tatcgatacc ctgcaaagct gggtgatgaa actggttcgc
    24301 ctggtcatgc agttgacccc ttacggcgtt ctggcactaa tgaccaaagt ggttgcaggt
    24361 tctaacctgc aagacatcat caaactggga agtttcgttg tcgcgtccta cctcggtctg
    24421 ctgattatgt ttgcagtgca tggcattctg ctgggcatta atggcgtgag tccgctgaag
    24481 tacttccgta aggtatggcc tgtgctgacg tttgccttta ccagccgttc cagtgctgcg
    24541 tctatcccac tgaatgtgga agcacaaacg cgtcgtctgg gcgttcctga atccatcgcc
    24601 agtttcgccg cctctttcgg tgcaaccatt ggtcagaacg gctgcgccgg tttgtatccg
    24661 gcaatgctgg cggtgatggt tgcgcctacg gttggcatta acccgctgga cccgatgtgg
    24721 attgcgacgc tggtcggtat tgttaccgtt agttccgcag gcgttgccgg tgtcggtggt
    24781 ggtgcaactt tcgccgcact gattgtgctg cctgcgatgg gcctgccagt aaccctggtg
    24841 gcgctgttaa tctccgttga accgcttatc gacatgggcc gtacggcgct aaacgttagt
    24901 ggctcgatga cagctggcac gctgaccagc cagtggctga agcaaaccga taaagccatt
    24961 ctggatagcg aagacgacgc cgaactggca caccgttaat tcttatgctg gcaacggtcc
    25021 gttttgtata ggggccgttg ctttactttt taaatgttcc atcgcggcga tgcatactga
    25081 ctcatattta aacagatcgt cagcgaaaaa aatgtccaac ttatcaatac attcctggct
    25141 caatgaatgt gcctgtagtg agaagcttaa aacctcatac ccgagacgtt ttctaagtga
    25201 actatctgga atactcataa tatcttcaag cacaccactt cccattcccg gaagatttag
    25261 tggattaaaa agaagattga tattaagcat tgatattatc gaaggaaact ttaggagact
    25321 ctcatttttt ttgaatcgca gcaaaaatgc tcccggcagg atgtatataa tttttttca
//
LOCUS       DAZFWL020000062        25332 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFWL020000062 DAZFWL020000000
VERSION     DAZFWL020000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.062"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..384)
                     /gene="sbmC"
                     /locus_tag="ACHKNP_RS13370"
                     /old_locus_tag="pgaptmp_002674"
     CDS             complement(<1..384)
                     /gene="sbmC"
                     /locus_tag="ACHKNP_RS13370"
                     /old_locus_tag="pgaptmp_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HGU4704813.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVFYDNPDETPAEKLRCDIVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPK"
     gene            complement(502..1668)
                     /gene="dacD"
                     /locus_tag="ACHKNP_RS13375"
                     /old_locus_tag="pgaptmp_002675"
     CDS             complement(502..1668)
                     /gene="dacD"
                     /locus_tag="ACHKNP_RS13375"
                     /old_locus_tag="pgaptmp_002675"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HGU4704814.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRTIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHDEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            1877..3304
                     /gene="sbcB"
                     /locus_tag="ACHKNP_RS13380"
                     /old_locus_tag="pgaptmp_002676"
     CDS             1877..3304
                     /gene="sbcB"
                     /locus_tag="ACHKNP_RS13380"
                     /old_locus_tag="pgaptmp_002676"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HGU4704815.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQETRAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(3347..3574)
                     /gene="tsuB"
                     /locus_tag="ACHKNP_RS13385"
                     /old_locus_tag="pgaptmp_002677"
     CDS             complement(3347..3574)
                     /gene="tsuB"
                     /locus_tag="ACHKNP_RS13385"
                     /old_locus_tag="pgaptmp_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HGU4704816.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(3588..4646)
                     /gene="tsuA"
                     /locus_tag="ACHKNP_RS13390"
                     /old_locus_tag="pgaptmp_002678"
     CDS             complement(3588..4646)
                     /gene="tsuA"
                     /locus_tag="ACHKNP_RS13390"
                     /old_locus_tag="pgaptmp_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HGU4704817.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(4825..6183)
                     /gene="plaP"
                     /locus_tag="ACHKNP_RS13395"
                     /old_locus_tag="pgaptmp_002679"
     CDS             complement(4825..6183)
                     /gene="plaP"
                     /locus_tag="ACHKNP_RS13395"
                     /old_locus_tag="pgaptmp_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HGU4704818.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(6173..6235)
                     /gene="yoeI"
                     /locus_tag="ACHKNP_RS13400"
                     /old_locus_tag="pgaptmp_002680"
     CDS             complement(6173..6235)
                     /gene="yoeI"
                     /locus_tag="ACHKNP_RS13400"
                     /old_locus_tag="pgaptmp_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HGU4704819.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(6450..7379)
                     /gene="yeeY"
                     /locus_tag="ACHKNP_RS13405"
                     /old_locus_tag="pgaptmp_002681"
     CDS             complement(6450..7379)
                     /gene="yeeY"
                     /locus_tag="ACHKNP_RS13405"
                     /old_locus_tag="pgaptmp_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4704820.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(7425..8249)
                     /gene="yeeZ"
                     /locus_tag="ACHKNP_RS13410"
                     /old_locus_tag="pgaptmp_002682"
     CDS             complement(7425..8249)
                     /gene="yeeZ"
                     /locus_tag="ACHKNP_RS13410"
                     /old_locus_tag="pgaptmp_002682"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4704821.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(8332..8586)
                     /gene="yoeB"
                     /locus_tag="ACHKNP_RS13415"
                     /old_locus_tag="pgaptmp_002683"
     CDS             complement(8332..8586)
                     /gene="yoeB"
                     /locus_tag="ACHKNP_RS13415"
                     /old_locus_tag="pgaptmp_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HGU4704822.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(8583..8834)
                     /gene="yefM"
                     /locus_tag="ACHKNP_RS13420"
                     /old_locus_tag="pgaptmp_002684"
     CDS             complement(8583..8834)
                     /gene="yefM"
                     /locus_tag="ACHKNP_RS13420"
                     /old_locus_tag="pgaptmp_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HGU4704823.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            9117..9164
                     /gene="hisL"
                     /locus_tag="ACHKNP_RS13425"
                     /old_locus_tag="pgaptmp_002685"
     CDS             9117..9164
                     /gene="hisL"
                     /locus_tag="ACHKNP_RS13425"
                     /old_locus_tag="pgaptmp_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HGU4704824.1"
                     /translation="MTRVQFKHHHHHHPD"
     misc_feature    9140..9263
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            9310..10209
                     /gene="hisG"
                     /locus_tag="ACHKNP_RS13430"
                     /old_locus_tag="pgaptmp_002686"
     CDS             9310..10209
                     /gene="hisG"
                     /locus_tag="ACHKNP_RS13430"
                     /old_locus_tag="pgaptmp_002686"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HGU4704825.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            10215..11519
                     /gene="hisD"
                     /locus_tag="ACHKNP_RS13435"
                     /old_locus_tag="pgaptmp_002687"
     CDS             10215..11519
                     /gene="hisD"
                     /locus_tag="ACHKNP_RS13435"
                     /old_locus_tag="pgaptmp_002687"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HGU4704826.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRQDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            11516..12586
                     /gene="hisC"
                     /locus_tag="ACHKNP_RS13440"
                     /old_locus_tag="pgaptmp_002688"
     CDS             11516..12586
                     /gene="hisC"
                     /locus_tag="ACHKNP_RS13440"
                     /old_locus_tag="pgaptmp_002688"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGU4704827.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPAAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            12586..13653
                     /gene="hisB"
                     /locus_tag="ACHKNP_RS13445"
                     /old_locus_tag="pgaptmp_002689"
     CDS             12586..13653
                     /gene="hisB"
                     /locus_tag="ACHKNP_RS13445"
                     /old_locus_tag="pgaptmp_002689"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HGU4704828.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGDSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            13653..14243
                     /gene="hisH"
                     /locus_tag="ACHKNP_RS13450"
                     /old_locus_tag="pgaptmp_002690"
     CDS             13653..14243
                     /gene="hisH"
                     /locus_tag="ACHKNP_RS13450"
                     /old_locus_tag="pgaptmp_002690"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HGU4704829.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFALPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            14243..14980
                     /gene="hisA"
                     /locus_tag="ACHKNP_RS13455"
                     /old_locus_tag="pgaptmp_002691"
     CDS             14243..14980
                     /gene="hisA"
                     /locus_tag="ACHKNP_RS13455"
                     /old_locus_tag="pgaptmp_002691"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HGU4704830.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     LLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVESY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            14962..15738
                     /gene="hisF"
                     /locus_tag="ACHKNP_RS13460"
                     /old_locus_tag="pgaptmp_002692"
     CDS             14962..15738
                     /gene="hisF"
                     /locus_tag="ACHKNP_RS13460"
                     /old_locus_tag="pgaptmp_002692"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HGU4704831.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCRVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            15732..16343
                     /gene="hisIE"
                     /locus_tag="ACHKNP_RS13465"
                     /old_locus_tag="pgaptmp_002693"
     CDS             15732..16343
                     /gene="hisIE"
                     /locus_tag="ACHKNP_RS13465"
                     /old_locus_tag="pgaptmp_002693"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HGU4704832.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(16440..17417)
                     /gene="wzzB"
                     /locus_tag="ACHKNP_RS13470"
                     /old_locus_tag="pgaptmp_002694"
     CDS             complement(16440..17417)
                     /gene="wzzB"
                     /locus_tag="ACHKNP_RS13470"
                     /old_locus_tag="pgaptmp_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HGU4704833.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(17564..18730)
                     /gene="ugd"
                     /locus_tag="ACHKNP_RS13475"
                     /old_locus_tag="pgaptmp_002695"
     CDS             complement(17564..18730)
                     /gene="ugd"
                     /locus_tag="ACHKNP_RS13475"
                     /old_locus_tag="pgaptmp_002695"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HGU4704834.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPNTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTKNIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPHVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(18979..20385)
                     /gene="gndA"
                     /locus_tag="ACHKNP_RS13480"
                     /old_locus_tag="pgaptmp_002696"
     CDS             complement(18979..20385)
                     /gene="gndA"
                     /locus_tag="ACHKNP_RS13480"
                     /old_locus_tag="pgaptmp_002696"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGU4704835.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(20496..20903)
                     /locus_tag="ACHKNP_RS13485"
                     /old_locus_tag="pgaptmp_002697"
     CDS             complement(20496..20903)
                     /locus_tag="ACHKNP_RS13485"
                     /old_locus_tag="pgaptmp_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HGU4704836.1"
                     /translation="MRLVDDNIMDELFRTAANSERLRAHYLLHASHQEKVQRLLIAFV
                     RDSYVEPHWHELPHQWEMFVVMQGQLEVCLYEQNGELQKKFVVGDGTGISVVEFSPGD
                     IHSVKCLSPKALMLEIKEGPFDPLKAKAFSKWL"
     gene            complement(20894..22102)
                     /locus_tag="ACHKNP_RS13490"
                     /old_locus_tag="pgaptmp_002698"
     CDS             complement(20894..22102)
                     /locus_tag="ACHKNP_RS13490"
                     /old_locus_tag="pgaptmp_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU4704837.1"
                     /translation="MKLALIIDDYLPHSTRVGAKMFHELGLELLSRGHDVTVITPDNT
                     LQAIYSVSMTDGIKVWRFKSGPLKDIGKAKRAINETLLSFRAWHALKHLIQHDTFDGI
                     VYYSPSIFWGGLVKKIKERCQCPSYLVLRDMFPQWVIDAGMIKAGSPIEKYFRYFEKK
                     SYQQADWIGLMSDKNLEIFRQANKGYPCEVLRNWASMTPVSAGDDYHSLRQKYALKDK
                     IIFFYGGNIGHAQDMANLMRLARNMMRHHDAHFLFIGQGDEVDLIKSLSAEWNLTNFT
                     HLPSVNQEEFKLILSEVDVGLFSLSSRHSSHNFPGKLLGYMVQSIPILGSVNGGNDLM
                     DVINKHRAGFIHVNGEDDKLFESAQLLLSDSVLRKQLGQNANVLLKSQFSVESAAHTI
                     EVRLEAGECV"
     gene            complement(22102..23232)
                     /gene="wecB"
                     /locus_tag="ACHKNP_RS13495"
                     /old_locus_tag="pgaptmp_002699"
     CDS             complement(22102..23232)
                     /gene="wecB"
                     /locus_tag="ACHKNP_RS13495"
                     /old_locus_tag="pgaptmp_002699"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGU4704838.1"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKLDEYCDHLIVHTGQNYDYE
                     LNEVFFKDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVFEQEKPEAMLVLGDTNSCI
                     SAIPAKRRRIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINMTYSDIAREYLLAEGV
                     PADRIIKTGSPMFEVLTHYMSQIDGSDVLSRLNLTPGNFFVVSAHREENVDTPKQLAK
                     LANILNTVAEKYDIPVVVSTHPRTRNRINENGIQFHKNILLLKPLGFHDYNHLQKNAR
                     AVLSDSGTITEESSIMNFPALNIREAHERPEGFEEGAVMMVGLESARVLQALEIIATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIIHSYTDYVKRVVWKQY"
     gene            complement(23229..24335)
                     /locus_tag="ACHKNP_RS13500"
                     /old_locus_tag="pgaptmp_002700"
     CDS             complement(23229..24335)
                     /locus_tag="ACHKNP_RS13500"
                     /old_locus_tag="pgaptmp_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HGU4704839.1"
                     /translation="MKILVTGAAGFIGRNLVFRLKEAGYNELIAIDRNSSLTDLEQGL
                     KQADFIFHLAGVNRPVKESEFEEGNCDLTQQIVDILKKNNKDTPIMLSSSIQAECDNA
                     YGKSKAVAEKIIQQYGETTNAKYYIYRLPNVFGKWCQPNYNSFIATFCHRIANDEAIT
                     INDPSAVVNLVYIDDFCSDILKLLEGANETGYRTFGPIYSVTVGEVAQLIYRFKESRQ
                     TLIIEDVGNGFTRALYSTWLSYLSPKQFAYTVPSYSDDRGVFCEVLKTKNAGQFSFFT
                     AHPGITRGGHYHHSKNEKFIIIRGSACFKFENIVTGERYELNVSSDDFKIVETVPGWT
                     HDITNNGSDELVVMLWANEIFNRSEPDTIARVLS"
     gene            complement(24337..>25332)
                     /gene="fnlA"
                     /locus_tag="ACHKNP_RS13505"
                     /old_locus_tag="pgaptmp_002701"
     CDS             complement(24337..>25332)
                     /gene="fnlA"
                     /locus_tag="ACHKNP_RS13505"
                     /old_locus_tag="pgaptmp_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     4,6-dehydratase/5-epimerase"
                     /protein_id="HGU4704840.1"
                     /translation="GSFGNAVLKRFLDTDITEIRIFSRDEKKQDDMRKKYNNSKLKFY
                     IGDVRDYNSVLNATRGADFLYHAAALKQVPSCEFHPMEAVKTNVLGTENVLEAAIANG
                     IKRVVCLSTDKAVYPINAMGISKAMMEKVIVAKSRNLDSSKTVICGTRYGNVMASRGS
                     VIPLFVDLIKAGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNNGDIFVQKAPAATI
                     QTLAIALKELLNAHEHPINIIGTRHGEKLYEALLSREEMIAAEDMGDYYRVPPDLRDL
                     NYGKYVEHGDRRISEVEDYNSHNTERLDVEGMKELLLKLPFIRALRSGEDYELDS"
BASE COUNT         6773 a         6331 c         5890 g         6338 t
ORIGIN      
        1 ctttggtaac atttcataag cactgtcctg caagaggcta ttaaagaact gataccaggg
       61 tttagcaaaa tcatcaccga ctacacgagc caccgcaacc gcatactgac cacctgaaat
      121 ttctgtcaga atgacgccct cactgttttc ggggagcgta aagttattcg gcaccgtcac
      181 gacgatgtcg cagcgtaatt tttcggcggg tgtttcatct ggattgtcgt aaaagacagc
      241 aacccactcc ttcggcacaa tatttttgct atctacccac atcatcaact gctcaaagcc
      301 tttctttacc gtctgttccc acgggccaac gagatggaaa cctgcaacgg tacgtttatc
      361 ttcctgctta atctcgtagt tcatgacgtc ccattaacac tgtttttata tacagtataa
      421 ttgcgatatt aaaaccacaa ggaatgagtg ttgattatgc gagcagactc gcactcctgc
      481 cagtctgctg caaaagaaag gtcaggcctt atggtggaaa taatcactca ggcgagaaaa
      541 catgctgcct tccccgacag attccagggt aaccagcggc cagtgcgcca cctgtttatc
      601 acggtcgtaa agttcaattt cccctacccg ctgatgggcg ctaattggcg cggtgagctc
      661 tttaccatca agggtatatt tggctttgat atgtggaatt tcggctttcg gtagcaccat
      721 ccagaactct tgttccgttc ccaggtcgat attttcttta tcgccatacc agatgcgttc
      781 cgtaccgacc tttttcccac ggtgcaaaat ttgcaccgta gtaaagtttt gttgccccca
      841 acgcagtaat tttcttgcct cttcctcacg accttttgca ctgtcagccc ccattaccac
      901 tgcaatgaga cgacgctgcc catcgacagc cgaagcaatg agattaaacc cggcaccaga
      961 agtatgaccc gttttcaggc cgtcaacatt catggtttta tcccacaata acccgttacg
     1021 gttttgctgg gtgataccgt tccaggtgag acttttctca ctgtacatat gataaaactc
     1081 gggctcgtcg tggatgatag cgcgagaaag cacagctaaa tcataagccg agctatgctg
     1141 acctggtgca tccagaccat gcactgtttc aaaatgcgta tccttgagat gcagcttctc
     1201 ggcatagttg ttcatcattt caacaaactg ccgttgccca ccggcaatat agtcagccag
     1261 tgcaacacaa gcgtcatttc cggaatccac aattaaacca cggcttaaat cacgtaccga
     1321 tacgcgatcg ccctctttca aaaacatcag tgaagaaccg acaaacactg gattatcttt
     1381 cgcccacgca tcgcgcccca cggtgacaat atcgtctggc gtaatgcgat gactatcgat
     1441 agtgcgatcc acgacatagc ccgtcatcag ctttgtcagg ctggcgggat tgcgctgttg
     1501 atgctcatta cccgcggtga ggatctgacc ggtggtgtaa tccatcagta cccaggagcc
     1561 ggcatgaatc tctggaggct gaggtgaaaa aggaatgttt tccgccgcaa aaccagacga
     1621 taagttaaaa atgaacaaag aagcagcaat aataagacgg cgtttcaaca gcaaaccctc
     1681 aggagtttca aatagctgtt ctttttacgg aaatacttat gaactggctg gaataaagtg
     1741 caagaaaatg tgactaccct ctcattttta tctgacatga tctgttgtca ctcgctgtca
     1801 aattgtcgcg ctaaagctga ttagcacggt gatatttgat actctggcag acagcagaaa
     1861 taacggattt aacctaatga tgaatgacgg taagcaacaa tctacctttt tgtttcacga
     1921 ttacgaaacc tttggcacgc accccgcgtt agatcgccct gcacagttcg cagccattcg
     1981 caccgatgac gaattcaatg tcatcggcga acccgaagtc ttttactgca agcccgcgga
     2041 tgactattta ccccagcctg gagcagtatt aattaccggt attaccccgc aggagacacg
     2101 agcgaaagga gaaaacgaag ccgcgtttgc cgcccgtatt cactcgcttt ttaccgtacc
     2161 gaagacctgt attctgggct acaacaatgt gcgtttcgac gacgaagtca cacgcaacgt
     2221 tttttatcgt aatttctacg atccttacgc ctggagctgg cagcatgata actcgcgctg
     2281 ggatttactg gatgttatgc gtgcctgtta tgccctgcgc ccggaaggaa taaattggcc
     2341 tgaaaatgat gacggtctac cgagctttcg ccttgagcat ttaaccaaag cgaatggtat
     2401 tgaacatagc aacgcccacg atgcgatggc tgatgtgtac gccactattg cgatggcaaa
     2461 actggtaaaa acgcgtcagc cacgcctgtt tgattatctc tttacccatc gtaataaaca
     2521 caaactaatg gcgttgattg atgttcctca gatgaaaccc ctggttcacg tttccggaat
     2581 gtttggtgca tggcgcggca atacaagctg ggtggcaccg ctggcgtggc atcctgaaaa
     2641 tcgcaatgcc gtaattatgg tggatttggc aggagacatt tcgccattac tggagctgga
     2701 tagcgacaca ttgcgcgagc gtttatatac cgcaaaagcc gatcttggcg ataacgccgc
     2761 cgttccggtt aagctggtac atatcaataa atgtccggta ctggcccagg cgaatacgct
     2821 acgcccggaa gatgccgacc gactgggaat taatcgtcag cattgcctcg ataacctaaa
     2881 aattctgcgt gaaaatccgc aagtgcgtga aaaagtggta gcgatattcg tggaagccga
     2941 accgtttaca ccttcagata atgtggatgc acagctttat aacggctttt tcagtgacgc
     3001 cgatcgtgca gcaatgaaaa ttgtgctgga aaccgagccg cgtaatttac cggcactgga
     3061 tatcactttt gttgataaac gaattgaaaa gctgttgttc aattatcggg cgcgcaactt
     3121 cccggggacg ctggattatg ccgagcagca acgctggctg gagcaccgcc gccaggtctt
     3181 cacgccagag tttttacaag gctatgcaga agagattcag atgctggcgc agcagtatgc
     3241 cgacgataaa gaaaaagtgg cgctgttaaa agcgctttgg cagtacgcgg aagagattgt
     3301 ctaatgtgaa gcctccgccg ctggtacggc ggaggacaat tttgagtcag gctttttgaa
     3361 cggtgatgct ccacgctgca tcgccaatct gctgataatc ggtgatggca tgtccttctt
     3421 ctgccgccca ctgtgggatt gcttcagttg cctgggtgca atcaaactca attaccaact
     3481 catcgccgct ggccatttct gccagtgctg cttttgcttc tatgagtgga aacggacaga
     3541 cctgcgtcac cacatccagc tttttaatcg ccatctttac accttactta atttgccgca
     3601 gcagttgcca gtcgtgcctt acgctgcggt cgaacataca caagccagga cgcagtccag
     3661 actccgagaa tcataaatac aaggccaatc cagccctgcc aggtcatcat tgcagtcatg
     3721 accagtccat taccgataga gcaaccacct gcaatgctgg caccgaagcc catcagtaca
     3781 ccacctaacc cgctacgtaa tgttgtttgt gcatcagcag cgcgaacgcg gaactcacgg
     3841 ctcgctttgg ctgcaataaa tgaccccacg aagatcccta acaccaggaa aacgccccag
     3901 ttaatgtatt tcacgtcacc cgcgaccaga aattgcagaa tattggccgt tggggaagtg
     3961 attcctaacc caaacatgcg tccggttgct tcactcaggg gccaagctaa aagcgcaatc
     4021 aaaccgatga gtacagcagt gacaaaggga tgccagcgtt tttcaaacag aatatgagcg
     4081 atcccggttc ggcgcggcgg taaggtcgcg actttgagtt ttggcttctt caactctttc
     4141 atcaccaccc agagcgttat caccagcaaa acggcaacca acggccacac agacaaatta
     4201 aaagtatcag caatagagtt atgttcagta gtgtagtgct gcaaggtttg atttaaacca
     4261 ctggcgtgtg gagaacgcat caccgcactc atcaccatat aagtgaaaag cgcgatccaa
     4321 ctgccgatca atccctcacc cgcgcgatac caggtcccgg tggcacatcc acccgccaga
     4381 acaattccca gcccgaaaag atacccacct ataacagtac caagccacgg gaacgccccg
     4441 gcttcgtaag tcagtaggcc cgcctgaatt aacgcaaaaa cccccacgct ttgtaccgaa
     4501 attgcaatca gcaaggcgta aaacatgcga ttatttttcg cgatatacat atcgcgaaaa
     4561 ccacctgtca ggcaaaaacg cccacgctgc atgacaaatc ccagcagagc accacaaatt
     4621 agcccgctta atatcattga aaacataaat taattaacca gatgaatgtt aatggggaaa
     4681 ttattcatga ctggtggaat gcagaccaat aaccaaattc tctaaattag aacaaatggt
     4741 tattaatgag gatttcgata aaaaagaata aaaaaaccgg agccaaatgt tcattcgact
     4801 ccggtttatt attagaaaag atgattatgc tacgtcttcg tactgcggaa ccggattacg
     4861 gaaactcttg gttacgcaag ccaggtaaat cagaccgata gcagcccaga tcagacccag
     4921 aaccattgag ctttcttcca ggttaaccca cagcgcacca acggtcagcg caccacacat
     4981 cggcaggaac agatactgga agtgatcttt cagcgtcttg ttacgcttct cacggatcca
     5041 gaactgcgaa ataaccgaca ggttaacgaa ggtgaacgcc accagcgcac caaagttaat
     5101 cagcgccgta gccattacca ggtcgaagtt gattgccagc aaggcaatcg cgccaaccag
     5161 gatgatgttc attgccggag tacgccattt cgggtgtaca taaccgaaga agcttttcgg
     5221 gaacacaccg tcacgcccca ttacgtacat cagacgcgct acgcctgcat gcgccgccat
     5281 accggacgcc agtacggtaa tggtggagaa gatcagcgcg ccaacctgga acgctttacc
     5341 cgcaacgtac agcatgattt caggctgtga cgcatccgga tctttaaagc gagagatatc
     5401 cgggaagtac agctgcagga agtaagttgc aaagatgaag atcatgccgc caatcagtgc
     5461 ggtcaggaaa atcgcacgcg ggatcacgcg ctctgcatct ttggtttctt ccgacaggtt
     5521 gctgatgccg tcaaagccgg taaaggagaa gcacaggatt gtcgccccgg taatcatcgg
     5581 gataacatgc gcatcgccag accagaacgg acgagtgctc gccagcgtac ccgcgccttc
     5641 accttcaaat acgccataaa caaccatgcc cagaatcacc gcgatcagca ctacctgcaa
     5701 cacgacgatt acagtgttga agttcgctac ggatttcaga ctacgcaggt taaaggcggt
     5761 cataaaggcc accagcgcca ccacaaacat ccacgatggg atggaaggca ccagagcttc
     5821 aaaatagatt ttcgccagca gaatgttgat catcggcgcg aacagatagt cgagcagaga
     5881 agaccaaccc accataaagc cgacagtcgg gctaatggat ttctgggcgt aagtgtatgc
     5941 agagccagca gaaggatagc ggcgaaccag cttcccgtag ctcagagccg taaacaggat
     6001 cgcaatcaat gcgaacgcat aggctgtcgg cacatgacca tccgtgaggc ctgaaacgat
     6061 accaaatgta tcaaacagcg tcatcggctg catataggca agacccatca ttacaaccgg
     6121 aactaacgta agcgttttac gcaattccac gcgagaggtg tttggagtaa cgttatgcga
     6181 catggtcatt ctcctttacg gtgataaccg tcgcgtaagc aaaaaattgc cccatttttt
     6241 tggattcctc agcgacaaca actgtcgatt tttagtaaat atctatccgg tacgaagccc
     6301 ggcctcttgg tatgaatgat tggtttgaag caaaaaataa ccgacgctaa ttaaacgtcg
     6361 gtttttagtc attttttgac agcggcgcat tgtgcctaaa tgggggggaa atgacaagag
     6421 aatgagaggc ttgtcataat aatttttctt taaatggctg attttccgtc atcagatttt
     6481 gcataaacac cggcgatcgc actatttgct aaaatttcgt cccgccacca accactggca
     6541 agccccgcgg tctgttcatt ccagccaatc cagacgggtt caaagagggt ttgggcgacc
     6601 actttcttct cgattaacgc accactatcg agaaaacgtt gcgccagata acggggtaaa
     6661 tagccgcagc cgaggccgct aatttgcagc tccagcttgg ttttaaaatc gaaaacggta
     6721 atagcttctt gttcgtcgag aagttgtgag gccgtagaag cgccggcctg tgcagtgtcc
     6781 cccaccacaa tcgcccgata tcgtttaatg atacgacggt ttaacggttc ttcttccagg
     6841 gctaacggat gatgaggcgc gaccgcaaag acttgttcca gatcgcccaa ccgagagaaa
     6901 ccaaactcac tggaagatgg tggctcatgc atcgcgccaa caataatatc cgctcgcccc
     6961 tgagtcaggg catcccatga accaccgagt acaccattga taaacttcag gcgcgtcacg
     7021 ctatgatgtt gataaaaagc ctcaatgagt ggcgcaagca gagaaaaagg gaaggtgtcg
     7081 tccacgccaa tcaccagctc gttttcccag ccttcatgca gtttgatcgc ctgcttttcc
     7141 agttctcgca cggtatgcag aacttcccgc cctttctcta ataacatttt tccggtgcgg
     7201 gtgaatttag cgcggtgtcc gctgcgatca agcaattgaa tattaaggtc gctttccagc
     7261 ttgtgaacgg tgtaactcag agcggacggc gtcttgtaga gtttggccga cgccgccgca
     7321 aaactgcctt ctttttctaa ggcatcgagg atcatcaaca catccagcag tggtttcata
     7381 cacgccccct tgcagtgtat gcggcgtcct gctggcgtga tcgtttactc cagcggcatt
     7441 accagcggat cgggatactg gtattcaaat cccagttcat tacaaatccg actgccatca
     7501 ataatcttac ctttgccgct gtccagacta tttctgaact gcggaggttc cagccccaat
     7561 aaacgggcca tctgcggata gaaaacatta cgcgcagggt gagctggcgc acatatatta
     7621 tagatgtgtc cgcctttagg tgcctgtaac aacagagtga tagcgccaat aacatcttct
     7681 aaatggacta aattaacacc atgttcacca tcaggggcgg tttttccggc aaagaagcgt
     7741 ccgggatgac gtcccggtcc gaccaggccc gcaagacgca gaatatcgac cgaagtaccg
     7801 ggtaaattgt gcagccagtc ttcgagttct tctaatactc gtccactgtt ggtcaccgga
     7861 ttacgcgggg tggtttcttt caccgtgcct tgcgcgtcgc catagacaga tgtcgagctg
     7921 gtaaaaataa tgcggggaat acgatgggcc agcgcgctat ccactaactc ttgtaccgct
     7981 tgtaaataga actcatcgcc ggggccgcta cgacgtgccg gaagcgtaat gaccagcgca
     8041 tcggcatcca tcagggcatc cagatcgtca gaatcgcaaa ctaactcagg ctccatgcga
     8101 agcagatagc tatcaatgcc actcattcgg gccgcttcga caccatcttg tgtggtttta
     8161 ctcccggtga cttgccagcc tcgcgctgaa agtgacatcg ccagcggcat gcctaaccac
     8221 cctaacccga caattgcgac ctttttcatc cgttatctcc tgatacacct tagatctata
     8281 aggctacgct agcgtatcaa aactgacaat tcattctatg aatgaatctg ttcaataatg
     8341 ataacgacat gctgcaatga gcagtgaatc atcagtaacc gcgtatacca gacggtgctc
     8401 ctctgtaatg cgtcgggacc agaaacctga caaattatgt ttcaggggtt ctggtttccc
     8461 cttaccttca aatggtgttc tgcgggtatc tttgataagt tcattgatct ttttaacaat
     8521 tcgcttatct gtttcctgcc agtacagata atcatcccat gattcctcag accagattag
     8581 tttcactcaa taatgtcctt ttccgttcct ttgcctgatt tcaggctatc gattgagtcc
     8641 atcaatctcc gggcgttagc gggggaacgc agtagataag ccgtctcttc cagcgagttg
     8701 tattcttcga gtgacatcag aacacaagcc tctccattct gacgagtaat gaggatcggg
     8761 gcatgatctt caacggcttt catcattgtt gccgacaaat tctgacgcgc ttcgctgtag
     8821 ctaattgtac gcatgtaaat ctcctctttt gtacagttca ttgtacaatg atgggtgtta
     8881 attcactatt tattaattag tttgtagatc aagacattgc cagcgagacg aaaaatcaga
     8941 ctttgctctt tttggtgctg tcaagttaga ggacagtcct cttagccccc tccttttccc
     9001 gctcattcat taaacaaatc catcgccata aaatatataa aaaagccctt gctttctaac
     9061 gtgaaagtgg tttaggttaa aagacatcag ttgaataaac attcacagag acttttatga
     9121 cacgcgttca atttaaacac catcatcacc atcatcctga ctagtctttc aggcgatgtg
     9181 tgctggaaga cattcagatc ttccagtggt gcatgaacgc atgagaaagc ccccggaaga
     9241 tcaccttccg ggggcttttt tattgcgcgg ttgttaacgg ttcagacagg tttaaagagg
     9301 aataagaaaa tgacagacaa cactcgttta cgcatagcta tgcagaaatc cggccgttta
     9361 agtgatgact cacgcgaatt actggcgcgc tgtggcatta aaatcaatct tcacacccag
     9421 cgcctgatcg cgatggcaga aaacatgccg attgatattc tgcgcgtgcg tgacgatgac
     9481 attcccggtc tggtaatgga cggcgtggta gaccttggga ttatcggcga aaacgtgctg
     9541 gaagaagagc tgcttaaccg ccgcgcccag ggtgaagatc cgcgctactt taccttgcgt
     9601 cgtctggatt tcggcggctg ccgcctttcg ctggcaacgc cggttgatga agcctgggac
     9661 ggtccgctct ccttaaatgg taaacgtatc gccacctctt atcctcacct gctcaagcgt
     9721 tatctcgacc agaaaggtat ctcttttaaa tcctgcttac tgaacggctc tgtcgaagtt
     9781 gcgccgcgtg ccggactggc ggatgcgatt tgcgatctgg tttccaccgg tgccacgctg
     9841 gaagctaacg gcctgcgcga agtcgaagtt atctaccgct cgaaagcctg cctgatccag
     9901 cgcgatggcg aaatggaaga atccaaacag caactgatcg acaaactgct gacccgtatt
     9961 cagggtgtga tccaggcgcg cgaatcaaaa tacatcatga tgcacgcacc gaccgaacgt
    10021 ctggatgaag tcatcgccct gctgccaggt gccgaacgcc caaccattct gccgctggcg
    10081 ggtgaccaac aacgcgtagc gatgcacatg gtcagtagcg aaaccctgtt ctgggaaacc
    10141 atggaaaaac tgaaagcgct gggtgccagt tcaatcctgg tcctgccgat tgagaagatg
    10201 atggagtgat cgccatgagc tttaacacaa tcattgactg gaatagctgt actgcggagc
    10261 aacaacgcca gctgttaatg cgcccggcga tctccgcttc tgaaagcatt acccgcaccg
    10321 ttaacgatat tctcgataac gtgaaagcac gcggcgatga cgccctgcgg gaatacagcg
    10381 cgaagtttga taaaaccacg gttaccgcgc tgaaggtgtc tgctgaggag atcgccgccg
    10441 ccagcgaacg ccttagcgac gagctaaaac aggcgatggc ggtggcagta aaaaatattg
    10501 aaaccttcca cactgcgcaa aaactgccgc cggtagatgt tgaaacacag ccaggcgtgc
    10561 gttgccagca agtcacgcgc ccggtagctt cagttgggtt gtatattcct ggcggctccg
    10621 ccccactctt ctcaacggta ttaatgctgg caactcctgc gcgtattgcg ggctgtaaaa
    10681 aagtggtgtt gtgctcaccg ccgccgattg ccgatgagat cctttatgcg gcgcagctgt
    10741 gcggtgtgca ggacgtgttt aacgtcggcg gcgcacaggc cattgccgct ctggcgtttg
    10801 gtacggaatc tgtgccgaaa gtggacaaaa tcttcgggcc gggtaacgcc tttgtcaccg
    10861 aagcgaaacg tcaggtgagc cagcgtcagg acggtgcggc gatcgatatg cccgcaggcc
    10921 cgtcggaagt gctggtgatt gctgacagcg gcgctacgcc agatttcgtg gcttctgatt
    10981 tgctttctca ggctgaacac ggcccggact cacaggtgat tttactgacg cccgctgctg
    11041 atatggcgcg tcgcgttgcc gaggccgtcg aacgccagtt ggcagaactg ccgcgtgccg
    11101 aaaccgcccg ccaggcactg aacgccagcc gcctaatcgt gaccaacgat ttagcacagt
    11161 gcgtggagat ctccaaccag tacggcccgg agcacctgat cattcagacc cgcaacgctc
    11221 gcgatctggt cgatagcatc accagcgccg gttcggtatt tcttggtgac tggtcaccgg
    11281 aatcggcagg tgattacgcc tccggcacca accacgttct gccgacttac ggttacaccg
    11341 ccacctgttc cagcctcggg ctggcagatt tccagaagcg catgaccgtg caggaactgt
    11401 cgaaagaagg cttttctgcg ctggcttcaa ccatagaaac actggccgcc gccgagcgcc
    11461 tgaccgccca taaaaatgcc gttactttgc gtgttaacgc ccttaaggag caagcatgag
    11521 caccgtgact attaccgatt tagcgcgtga aaacgtccgc aacctgacgc cgtatcagtc
    11581 ggcgcgtcgt ctgggcggta acggcgacgt ctggctgaac gccaacgaat atcccgcagc
    11641 cgtggagttt cagcttactc aacaaacgct caaccgctac ccggaatgcc agccgaaagc
    11701 ggtgattgaa aattacgcgc aatatgcagg cgtaaaaccg gagcaggtgc tggtcagccg
    11761 tggcgcggac gaaggtattg aactgctgat tcgcgctttt tgcgaaccgg gtaaagacgc
    11821 catcctctac tgcccgccaa cgtacggcat gtacagcgtc agcgccgaaa cgattggcgt
    11881 cgagtgtcgc acagtgccga cgcaggacaa ctggcaactg gacttacagg gcatttccga
    11941 caagctggac ggcgtaaaag tggtctatgt ttgcagcccc aacaacccga ccgggcaact
    12001 gatcaatccg caggattttc gcactctgct ggagctaacc cgcggaaaag ccattgtggt
    12061 tgccgatgaa gcctatatag agttttgtcc acaggcatcg ctggctggct ggctggcgga
    12121 atatccgcac ctggctattt tgcgcacact atcgaaagct tttgctctgg cggggcttcg
    12181 ttgcggattt acgctggcaa acgaagaagt catcaacctg ctgatgaaag tgatcgcccc
    12241 ctacccgctc tcgacgccgg ttgccgacat tgcggcccag gcgttaagcc cgcaggggat
    12301 cgtcgccatg cgcgaacggg tggcgcaaat tattgcagaa cgcgaatacc tgattgccgc
    12361 actgaaagag atcccctgcg tggagcaggt ttttgactct gaaaccaact acattctggc
    12421 gcgctttaaa gcctccagtg cagtgtttaa atctttgtgg gatcagggca ttatcttacg
    12481 tgatcagaat aaacaaccct ctttaagcgg ctgcctgcga attaccgtcg gaacccgtga
    12541 agaaagccag cgcgtcattg acgccttacg tgcggagcaa gtttaatgag tcagaagtat
    12601 ctttttatcg atcgcgatgg aaccctgatt agcgaaccgc cgagtgattt tcaggtggac
    12661 cgttttgaca aactcgcctt tgaaccgggc gtgatcccgg agctgctgaa gttgcaaaaa
    12721 gcgggctaca aactggtgat gatcactaat caggatggtc tgggaacaca aagtttcccg
    12781 caggcggatt tcgatggccc gcacaacctg atgatgcaga tcttcacctc gcaaggcgta
    12841 cagtttgatg aagtgctgat ttgtccgcac ctgcccgccg atgagtgcga ctgccgtaag
    12901 ccgaaagtaa aactggtgga gcgttatctc gctgagcaag cgatggatcg tgccaatagt
    12961 tatgtgattg gcgatcgcgc gaccgacatt caactggcgg aaaacatggg tattaatggt
    13021 ttacgctacg accgcgaaac cctgaactgg ccaatgattg gcgagcaact cactaaacga
    13081 gaccgttacg cccatgtagt gcgtaacacc aaagagacgc aaattgacgt ccaggtgtgg
    13141 ctggatcgcg aaggtgacag caagattaac accggcgttg gcttctttga tcacatgctg
    13201 gatcagatcg ccacccacgg cggtttccgt atggaaatca acgtcaaagg cgacctctat
    13261 atcgacgatc accacacagt cgaagatacc ggcctggcgc tgggtgaagc gttaaaaatt
    13321 gcccttggcg acaaacgcgg tatttgccgc tttggttttg tgctaccgat ggacgaatgc
    13381 cttgcccgct gcgcgctgga tatctctggt cgcccgcacc tggaatataa agccgagttt
    13441 acctaccagc gcgtgggcga tctcagcacc gaaatgatcg agcacttctt ccgttcgctc
    13501 tcttacacca tgggcgtgac gctacacctg aaaaccaaag gtaaaaacga tcatcaccgt
    13561 gtagagagcc tgttcaaagc ctttggtcgc accctgcgcc aggccatccg cgtggaaggc
    13621 gacaccctgc cctcgtcaaa aggagtgctg taatgaacgt ggtgatcctt gataccggct
    13681 gcgccaatct gaactcggtg aagtctgcca ttgcgcgcca cggttatgaa cccaaagtca
    13741 gccgtgaccc ggacgtcgtg ttgctggccg ataaactgtt tttacccggc gtaggcaccg
    13801 cacaagcagc gatggatcag gtgcgtgagc gcgaactgtt tgatctcatc aaagcctgta
    13861 cccaaccggt gctgggaatc tgcttaggga tgcaactgct ggggcggcgc agcgaagaga
    13921 gcaacggcgt cgacttgctg ggcatcattg acgaagacgt gccgaaaatg accgactttg
    13981 ctctgccact gccacatatg ggctggaacc gcgtttatcc gcaggcaggc aaccgcctgt
    14041 ttcagggaat tgaagacggc gcgtactttt actttgttca cagttacgca atgccggtca
    14101 atccgtggac catcgcccag tgtaattacg gcgaaccgtt caccgcggcg gtacaaaaag
    14161 ataacttcta cggcgtgcag ttccacccgg agcgttctgg tgccgctggc gctaagttgc
    14221 tgaaaaactt cctggagatg tgatgattat tccggcatta gatttaatcg acggcactgt
    14281 ggtgcgtctc catcagggcg attacggcaa acagcgcgat tatggtaacg acccgctgcc
    14341 gcgattgcag gattacgccg cgcagggtgc agaattgttg catctggtgg atctgaccgg
    14401 ggcaaaagat ccggctaaac gtcaaatccc gctgattaaa accctggtgg cgggcgttaa
    14461 cgtgccggtg caggttggcg gtggcgtgcg tagcgaagaa gacgtggcgg cgttactgga
    14521 agcaggcgtt gcgcgcgtgg tggttggctc caccgcggtg aaatcaccag agatggtgaa
    14581 aggctggttt gaacgtttcg gtgccgatgc cttagtgctg gcgctggatg tccgtattga
    14641 cgagcaaggc aacaagcagg tggcagtcag cggctggcaa gagaactcgg gcgtttcact
    14701 ggaacaactg gtggaaagct atctgcccgt cggcctgaaa catgtgctgt gtaccgatat
    14761 ctcgcgcgac ggcacgctgg caggttctaa cgtctcttta tatgaagaag tgtgcgccag
    14821 atatccgcag gtggcgtttc agtcctccgg cggtattggc gacattgatg atgtggcggc
    14881 cctgcgtggt actggcgtgc gcggcgtaat agttggtcgt gcattactgg aaggtaaatt
    14941 caccgtgaag gaggccatcg catgctggca aaacgcataa taccgtgtct cgacgtccgt
    15001 gatggtcagg tggtgaaagg cgtacagttt cgcaaccatg aaatcattgg cgatatcgtg
    15061 ccgctggcaa aacgctacgc tgaagaaggt gcagacgaac tggtgttcta cgatatcacc
    15121 gcttccagcg atggccgtgt ggtagataaa agctgggtat ctcgcgtggc ggaagtgatc
    15181 gacattccgt tctgcgtggc gggtgggatt aagtctctgg aagatgccgc gaaaattctt
    15241 tcctttggcg cggataaaat ttccatcaac tcccctgcgc tggcagaccc aacattaatt
    15301 actcgcctgg cggatcgttt tggcgtgcag tgtattgtgg tgggtattga tacctggtac
    15361 gacgctgaaa ccggtaaata tcatgtgaat caatataccg gcgatgaaag ccgcacccgc
    15421 gtcactcagt gggaaacact cgactgggta caggaagtgc aaaaacgcgg tgccggagaa
    15481 atcgtcctca acatgatgaa tcaggacggc gtgcgtaacg gttacgacct cgagcagctg
    15541 aaaaaagtgc gagaagtttg ccgcgtcccg ctgattgcct ccggtggcgc gggcaccatg
    15601 gaacacttcc tcgaagcctt ccgtgatgcc gacgttgacg gcgcgctggc ggcttccgta
    15661 ttccacaaac aaataatcaa tattggtgaa ttaaaagcgt acctggcaac acagggcgtg
    15721 gagatcagga tatgttaaca gaacaacaac gtcgcgaact ggactgggaa aaaaccgacg
    15781 gactgatgcc ggtgattgtg caacacgcgg tatctggcga agtgctaatg ctgggctata
    15841 tgaacccgga agccttagac aaaaccatcg aaagcggcaa agtcactttc ttctcgcgca
    15901 ctaaacagcg actgtggacc aaaggcgaaa cgtcgggcaa tttcctcaac gtagtaaata
    15961 ttgcgccgga ctgcgacaac gacacgctac tggtactggc gaatcccatc gggccaacct
    16021 gccacaaagg caccagcagc tgcttcggcg acaccgctca ccagtggctg ttcctgtatc
    16081 aactggaaca attgctcgcc gaacgcaaat ctgccgatcc ggaaacctct tacacagcca
    16141 aactgtatgc cagcggcacc aaacgcattg cacagaaagt gggcgaagag ggcgtggaaa
    16201 ccgcactagc agcaacggta catgaccgct ttgaactcac caacgaggcg tctgacttga
    16261 tgtatcacct gttggtgttg ttgcaggatc aggatctgga tttaacaacg gtgattgaga
    16321 acctgcgtaa acggcatcag tgagttgcgg ggtaagcgga tgcgatgttg ttgtcgcatc
    16381 cggcaaaaaa aacgggcaag gtgtcaccac cctgcccgtt ttctttaaaa ccaaaaagat
    16441 tacttcgcgt tgtaattacg cagagcgtta cgccccagca caatccccgc gccaaccatg
    16501 ccgcccagca gcaccgccag aatcaaagtg attgcctttt tcgggctatc gcgacgaata
    16561 ggtaacgtcg gtttcatcac atagcggtaa gcatgaatat caagatcatc aacttttaag
    16621 ctttcgatat ccagcaggtt ttgacgtgtc tgatagtagt ttggtgagaa caccaacgga
    16681 cgagtcgctt catgcttaat catcgactcc agcgcttcgc tccccaaaag gaacaacgta
    16741 tcttgtgtca catcttcagt ctgctgaacc tgtggctttg tcacctgcgc ctgattcgca
    16801 tactgcaacg cttcctgaat ctgacggata cgcagatctt tctgctcctg cgcaactact
    16861 tcctgagttt tcagagaatc ctgcaagttt ttcatacgca gcgcgatgtt gtctttcagg
    16921 tcttttccca gttcttcatt tacctggtcg tcaacttgct ggatatactt cgccagctgc
    16981 ttctgagcac cttcaggtgt ttcaccaaca taagacaccg ctaaaggtaa tgactgattt
    17041 ttgacggtag gctcaatggt cagcttttct gcttcttcct ggttatccag cgtttccgct
    17101 aatgctgaga atgcagtgct gtaacgtccg ataagtgaag cctgaatgtc ggagactttc
    17161 ggtacagccg atccgtagat cacattcatc gcgttggtat aaccagcgat ttgccccaca
    17221 tcaggttgag taacgatagc tgtggaagtc cacttttctt tggcaacggc cagataccct
    17281 atagccaggg caatagcaac aatgacagag atgataattg tcatcttgcc acgccacaac
    17341 tgcaccagta aatcaatcaa atcaatctgt tccgggtcat ggttttgccc agaaacatta
    17401 ttattttcta ctctcatcat taccctaacg gacgaaataa ttttaagtcg ccatagttta
    17461 gcgataatcg ccatgaatgc tataggataa atgataaaaa taatgaatta ttacaaaggg
    17521 aacataagag aatcagggcg aaaatcgccc tgataacagg atgttagtca ctgccaaaga
    17581 gatcgcgggt gtagactttg tctgccacat ccttaagctc ttctgccata cggttggaga
    17641 taattacgtc ggcttgttgt ttgaaagtgg cgagatcacg ttccagccga gagttgaaga
    17701 atgagtcttc tttcatcact ggctcgtaga tgattacttc aacacctttc gccttgatac
    17761 gcttcataat cccctgaata gaagacgcac ggaagttatc tgaaccgctc ttcataatca
    17821 ggcgataaat acccaccaca tgcggtttgc gtgacaaaat ggcatcggca ataaaatctt
    17881 tacgcgtgcg gtttgcatcg acaattgccg agatcaggtt attcggcaca gactgatagt
    17941 tcgccagtaa ctgcttggta tctttcggca gacaataacc accataacca aacgacggat
    18001 tgttgtaatg gttgccaata cgcggatcga gacaaacgcc ttcgataatc tggcgagtat
    18061 tcagacctaa actttctgcg tagctatcca gttcattaaa gtacgctacg cgcatcgcca
    18121 gataggtatt agcgaaaagt ttaatcgctt ctgcttcggt tgagtcggta aacagggtcg
    18181 ggatattttg cttaatcgcg ccttcctgta acagagcagc aaaacgttct gcgcgttctg
    18241 aacgctcacc gatgacaata cgtgaaggat ggagattatc gtaaagggct ttaccctcac
    18301 ggagaaattc cggagagaaa ataatatttt tagtacgata tttcttatgc atcgctgcgg
    18361 taaagccaac gggtaccgtt gatttgatga ccataaccgc ataaggattt atctctacta
    18421 cgtccttaat tactgattct acactcgatg tattgaaata attagtgtta ggatcatagt
    18481 cggttggagt ggcgatgatg acataatcag catcccggta ggcttcattt ttatctaatg
    18541 tggcattaaa gtgtatttta tctgattgca aaaactgctg aatttcctta tcaacaatag
    18601 gagatatccg atcattcagc atagcaacgc gtgacggtaa aatatctaat gccacaacct
    18661 catgattttg tgcgattaga agcccgtttg acaagcctac atagccggta ccggaaatgg
    18721 tgattttcat ttcatgctct cagaattaac ttaactgtga atcatgatgt ttttagcatc
    18781 ctgataagag ctaaaagttt taacgctacg ttgttcgatg gtcaacgcaa cgaaaaacaa
    18841 ttcagatttt ctttataaga atagctgata tttattaagt taatattaag cagtgaaatt
    18901 tagtctataa gataattggc aaaaaaaaag cccggcgtaa tatacgccgg gcctcaattt
    18961 attgttggtt aaatcagatt aatccagcca ttcagtatgg aacacacctt ctttatcaat
    19021 gcgcttataa gtatgcgcac caaaatagtc acgctgagcc tggatcaggt tcgcaggcag
    19081 aacagcggca cggtagctgt cataataggc aaccgcagcg gcgaaggtcg gaaccgggat
    19141 accgttctgt actgcataag caacgacatc acgcagcgcc tgctggtagt catcggcaat
    19201 ttgcttgaag tacggagcca acagcaggtt agcgatctga ggattttcgg cataagcatc
    19261 agttattttc tgcaggaact gcgcacggat gatgcagcca gcacggaaaa tcttcgcgat
    19321 ttcgccgtag ttcagatccc agttgtactc ttcagacgct gcgcgcagct gagagaagcc
    19381 ctgagcgtaa gaaacgattt tgcccagata cagcgcacgg cgaacttttt cgatgaactc
    19441 agccttgtcg ccagctggct gcgcttgcgg gccagagaga actttagatg cggcaacacg
    19501 ctgatctttc agagaagaga tataacgtgc aaacacagac tcggtaatca gcgacagcgg
    19561 ttcaccgaga tccagcgcgc tctggctggt ccatttaccg gtacctttgt tagccgcttc
    19621 atccaggatc acatcaacca ggtagttacc gtcttcatct tttttggtga agatgtcttt
    19681 ggtgatgtcg atcaggtagc tactcagttc accgttattc cactcggtaa aggtctgtgc
    19741 cagttcttcg ttagagagat tcaggccgcc tttcaggaga gaataggctt cagcaatcag
    19801 ttgcatatca ccgtattcaa taccgttgtg aaccatcttc acatagtgac ccgcgccatc
    19861 agcaccaata taggtaacgc acggttcgcc atcttcagca acggcggcga ttttagtcag
    19921 gatcggtgca accagttcat aggcttcttt ctgcccacca ggcataatgg aaggaccttt
    19981 cagtgcgcct tcttcaccac cggagacacc ggtaccaatg aagttaaaac cttctgcaga
    20041 aagctcacga ttacgacgaa tggtgtcctg gaagaaggta ttaccaccat caatgatgat
    20101 gtcaccttta tcgaggtatg gcttaaggga atcaatagca gcatccgtgc ctgcacctgc
    20161 tttcaccatt aacaggatgc gacgaggcgt ttccagagat tcaacaaact ctttcaccgt
    20221 atagtaagga accagtttct tgcctggatt ttcggcaatc acttcttccg tcttttcacg
    20281 ggaacggttg aaaatagaga cggtataacc acggctttcg atgttgagcg caaggttgcg
    20341 ccccatcact gccataccga ctacgccgat ctgttgcttt gacattgttt actcctgtca
    20401 ggggtggtca cgcggggtta cgcggcttga agaagctaag atggtaaccc agcatgtata
    20461 gaataagata gaagaataaa cggtggtgta tcgccctata accacttaga aaaagcctta
    20521 gctttcagtg ggtcaaatgg cccctccttt atctctaaca taagggcttt tggtgacagg
    20581 catttgacac tatgtatatc tccaggggaa aattccacga cacttattcc cgtaccgtct
    20641 ccaacaacaa actttttttg gagctcacca ttttgctcat acaaacaaac ttctaattgc
    20701 ccttgcatga cgacaaacat ttcccactga tgcggtaact catgccaatg gggttcaaca
    20761 tagctgtcgc gtacaaatgc aataagtaaa cgttgaacct tctcctgatg agatgcgtgc
    20821 aataaataat gagcgcgcaa acgttcagaa tttgctgcag tgcgaaaaag ttcatccata
    20881 atattgtcat caactaaacg cattctcctg cctccagtcg gacttcgata gtatgtgccg
    20941 ccgattcaac cgaaaattga gactttaaca acacattggc gttctgacct aactgttttc
    21001 ttaaaactga atcactaaga agcaattgtg cagattcaaa cagtttatca tcttcaccat
    21061 taacatgaat gaacccagcc ctgtgcttat taattacatc cattagatca ttaccgccat
    21121 tcacactccc aaggatcggg attgattgaa ccatataccc tagtaatttt ccggggaaat
    21181 tatgtgaaga atggcgagac gaaagggaga acaggccaac atcaacttca gataaaatta
    21241 atttaaactc ttcctggttc actgaaggta gatgagtgaa attagttaaa ttccattctg
    21301 cagaaagaga tttaatcagg tcaacttcat caccctgccc tataaacagg aaatgagcat
    21361 catgatgacg catcatatta cgcgcaaggc gcattaagtt tgccatatcc tgagcatgcc
    21421 caatattacc gccatagaaa aaaatgattt tatcttttag agcgtatttt tgacgaagtg
    21481 aatgataatc atcgccggca gacacaggag tcattgaggc ccaattacgt aaaacttcac
    21541 acggataacc tttattggcc tgacgaaata tctcaagatt cttatcagac attaacccaa
    21601 tccagtcagc ctgttgatat gatttttttt caaaatacct gaaatatttt tcaattggcg
    21661 agccggcttt tatcatacca gcatcaatga cccactgtgg gaacatatcc cttaggacca
    21721 gatagcttgg gcactggcat cgctctttaa tttttttaac caagcctccc caaaaaatag
    21781 agggggaata ataaacgata ccatcaaatg tatcatgttg gatgagatgc tttaatgcgt
    21841 gccatgcacg aaaagataaa agagtttcat ttatggcacg tttagcctta cctatatcct
    21901 ttaaaggtcc acttttgaaa cgccaaacct ttataccatc agtcatgcta acagaataga
    21961 ttgcctgtaa tgtgttgtca ggcgtaatta cagttacatc atggcctctg ctcagcaatt
    22021 ccaggcctaa ctcatgaaac attttagccc caactcgtgt gctatggggc aaataatcat
    22081 caatgattaa tgcaagtttc attagtattg cttccagaca acccgtttaa cgtagtcagt
    22141 atatgaatgg ataatacgca caactttatc tgaaacattt ggcatactat agtcactgac
    22201 ctgacgaagt aagcgtactt ctccacgagg ctgtgttgca ataatttcta atgcctgtaa
    22261 cacacgggca gactcaagac caaccatcat tacagcccct tcttcgaagc cttcagggcg
    22321 ttcgtgcgct tctcgtatat tgagcgcagg gaagttcata atggaggact cctctgtaat
    22381 agtcccgcta tccgataaaa cagcacgtgc atttttttgc agatggttgt aatcgtgaaa
    22441 tcctaatggc ttaagtagca agatattttt atgaaattga ataccgtttt cgttgatgcg
    22501 gttacgagtg cgcggatgag tagaaacaac taccgggata tcatattttt cagccacggt
    22561 attaagtata tttgccagtt tcgcaagctg tttaggggta tcaacatttt cttctctgtg
    22621 ggcacttacc acaaagaaat tcccaggtgt taaattcagg cgagaaagta catcggaacc
    22681 atcaatctgc gacatataat gagtgagtac ttcaaacatt gggctaccgg ttttaataat
    22741 cctatcggct ggtacacctt cagccaaaag atattcacgc gcgatatcac tatatgtcat
    22801 attgatatca gcggtgtggt caactatttt tctgttagtt tcttccggta cgcgttgatc
    22861 aaaacaacga ttcccagcct ccatatggaa aatcggaatt cttcgacgct ttgctggtat
    22921 tgctgaaata caggagttag tatcgccaag aactaacatg gcttctggtt tttcctgttc
    22981 aaagacctca tcaactttaa tgataacttg tccaatagtc tctgctgcat ttttacctgc
    23041 ggcattaaga aaataatcag gtttgcgaac acccaaatcc ttgaaaaaaa cctcattcaa
    23101 ttcataatcg taattttgcc cagtatgaac aataaggtga tcacaatatt catctaattt
    23161 tgcaaggaca cgcgagagtc gaataatttc tggacgagtc ccaacaaccg acatgacttt
    23221 caattttttc acgataaaac tctcgctata gtatctggtt cagaacgatt aaatatttca
    23281 tttgcccaaa gcataacaac tagctcatcc gagccattat tagtaatgtc atgcgtccat
    23341 cccggaactg tttcaacaat tttaaaatca tctgatgaaa cattaagttc atatcgttca
    23401 cccgtgacaa tattttcaaa tttgaaacaa gcacttcctc ggatgataat aaatttctca
    23461 tttttggaat gatgataatg ccccccccga gtaattcctg gatgcgcagt aaaaaacgaa
    23521 aactggcccg cgtttttcgt tttcaatact tcacagaata cccctctgtc atcactataa
    23581 gaaggaaccg tatacgcaaa ctgtttagga gaaaggtaac ttaaccatgt tgagtacaat
    23641 gctcgagtga atccattacc tacatcttcg atgattaatg tttggcgact ttctttaaat
    23701 cgataaatta attgtgccac ctcaccaaca gtaacagaat aaattggacc aaatgtcctg
    23761 taaccagttt cgttcgctcc ttctaatagc tttaatatgt cagaacaaaa gtcatctata
    23821 tacaccaaat ttacaactgc tgaaggatca ttaattgtaa tagcttcatc atttgcaatg
    23881 cgatggcaga aagttgctat aaaggagtta taatttggtt gacaccactt accgaataca
    23941 ttcggcaagc gataaatata atatttagcg tttgtcgttt ccccatactg ctgaatgatt
    24001 ttttccgcaa ctgctttact ctttccataa gcgttatcac attcagcttg gatggatgaa
    24061 ctaagcatga taggagtatc tttattattt tttttcagaa tatcaacaat ctgttgagtt
    24121 agatcgcaat tcccctcttc aaactcactc tccttcacag gacgatttac tccagcaaga
    24181 tgaaaaatga aatctgcctg cttaagtccc tgctctaaat ccgtcaaaga agagttacga
    24241 tctatcgcaa taagttcgtt atatccagct tccttaaggc ggaaaaccaa atttcgaccg
    24301 ataaaccctg cagcgccagt aactaaaatt ttcatattat gaatccaact cataatcttc
    24361 accagaacga agtgcccgga taaaaggaag ttttagcagt aattctttca ttccctcaac
    24421 atctaatctc tcagtattat gagaattata atcttccact tccgagatac gacggtcacc
    24481 atgttccaca tattttccat agttcaaatc gcggagatct ggtggaacac gataataatc
    24541 acccatatct tccgctgcaa tcatttcctc tcggctcaat aacgcttcgt aaagtttttc
    24601 cccgtgtcga gttccaataa tattgattgg atgctcatgg gcatttagca attccttaag
    24661 tgcaatggct aatgtttgaa ttgttgccgc aggtgctttc tgaacgaaaa tgtcaccatt
    24721 atttccatgt tcgaaagcat aaaggaccag atcgacagca tcctcaagcg tcatcatgaa
    24781 acgagtcata ttgggatcgg ttatggtcaa tggtttacca gctttgatta gatcaacaaa
    24841 taatgggatg accgatccac gtgaagccat tacatttcca taacgagttc cacagataac
    24901 tgtttttgaa ctgtcaagat tacgtgattt tgcaacaata actttttcca tcattgcctt
    24961 agatatgccc attgcattga taggataaac ggctttatcg gtactcaagc acaccacgcg
    25021 tttaatccca ttcgcaatag cagcctccag aacattttcc gtacccagaa catttgtctt
    25081 aaccgcctcc atagggtgaa attcacatga aggaacttgt ttaagggctg ctgcatgata
    25141 cagaaaatcg gcaccacgcg ttgcatttag aacggaatta tagtctcgca catcacctat
    25201 ataaaatttt aattttgagt tattatattt tttccgcata tcatcttgtt ttttttcatc
    25261 cctgctaaat attcgtattt cagtaatatc tgtatctaga aaacgcttaa gtacggcatt
    25321 accaaaagaa cc
//
LOCUS       DAZFWL020000063        24732 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFWL020000063 DAZFWL020000000
VERSION     DAZFWL020000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.063"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            74..445
                     /locus_tag="ACHKNP_RS13510"
                     /old_locus_tag="pgaptmp_002702"
     CDS             74..445
                     /locus_tag="ACHKNP_RS13510"
                     /old_locus_tag="pgaptmp_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704841.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGVSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            466..657
                     /locus_tag="ACHKNP_RS13515"
                     /old_locus_tag="pgaptmp_002703"
     CDS             466..657
                     /locus_tag="ACHKNP_RS13515"
                     /old_locus_tag="pgaptmp_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HGU4704842.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            659..937
                     /locus_tag="ACHKNP_RS13520"
                     /old_locus_tag="pgaptmp_002704"
     CDS             659..937
                     /locus_tag="ACHKNP_RS13520"
                     /old_locus_tag="pgaptmp_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4704843.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
     gene            1224..1376
                     /locus_tag="ACHKNP_RS13525"
                     /old_locus_tag="pgaptmp_002705"
     CDS             1224..1376
                     /locus_tag="ACHKNP_RS13525"
                     /old_locus_tag="pgaptmp_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4704844.1"
                     /translation="MDTIDLGNNASLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LAKNTS"
     gene            1797..2018
                     /gene="kilR"
                     /locus_tag="ACHKNP_RS13530"
                     /old_locus_tag="pgaptmp_002706"
     CDS             1797..2018
                     /gene="kilR"
                     /locus_tag="ACHKNP_RS13530"
                     /old_locus_tag="pgaptmp_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HGU4704845.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            2018..2188
                     /locus_tag="ACHKNP_RS13535"
                     /old_locus_tag="pgaptmp_002707"
     CDS             2018..2188
                     /locus_tag="ACHKNP_RS13535"
                     /old_locus_tag="pgaptmp_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HGU4704846.1"
                     /translation="MTKKIKCAYHLCNKGIEESKSIKRPLHFMRGVIPTTEMKKYCSE
                     NCAEKDQMAHEL"
     gene            2262..2537
                     /locus_tag="ACHKNP_RS13540"
                     /old_locus_tag="pgaptmp_002708"
     CDS             2262..2537
                     /locus_tag="ACHKNP_RS13540"
                     /old_locus_tag="pgaptmp_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704847.1"
                     /translation="MITNYEVTVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVTKMSLVHIGKNLPAEKKAGFLATLIAMKLKGEI"
     gene            2636..5287
                     /gene="recE"
                     /locus_tag="ACHKNP_RS13545"
                     /old_locus_tag="pgaptmp_002709"
     CDS             2636..5287
                     /gene="recE"
                     /locus_tag="ACHKNP_RS13545"
                     /old_locus_tag="pgaptmp_002709"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HGU4704848.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTCATLDYLIVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQE
                     DAPETEEPTGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRSMAEPTTLDNAALLRLTDAIKAVFSITKKHQ
                     PYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRITRTASGANAGGGNLTDRGEGFVH
                     DLTSLARDVATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGL
                     DYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMP
                     QRVTEEGKQDDEEKPQPSGAMADEQATAETVEPNATEHHQNTQPLDAQSQVNSVDAKY
                     QELRAELHEARKNIPSKNPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDSVYQN
                     QPETEKISPDAKQPEPVVQQEPEIVCNACGQTGGDNCPDCGAVMGDATYQETFGEENQ
                     VEAKEKDPEEMEGAEHPHNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNEN
                     YHAGPGVSKSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRF
                     IVAPEFNRRTNAGKEEEKAFLMECASTGKTVITAEEGRKIELMYQRVMALPLGQWLVE
                     SAGHAESSIYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQ
                     DAFYSDGYEAQFGVLPTFVFLVASTTVECGRYPVEIFMMGEEAKLAGQQEYHRNLRTL
                     ADCLNTDEWPAIKTLSLPRWAKEYAND"
     gene            5280..6089
                     /gene="recT"
                     /locus_tag="ACHKNP_RS13550"
                     /old_locus_tag="pgaptmp_002710"
     CDS             5280..6089
                     /gene="recT"
                     /locus_tag="ACHKNP_RS13550"
                     /old_locus_tag="pgaptmp_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HGU4704849.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            6146..6340
                     /gene="ralR"
                     /locus_tag="ACHKNP_RS13555"
                     /old_locus_tag="pgaptmp_002711"
     CDS             6146..6340
                     /gene="ralR"
                     /locus_tag="ACHKNP_RS13555"
                     /old_locus_tag="pgaptmp_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HGU4704850.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            6333..6521
                     /locus_tag="ACHKNP_RS13560"
                     /old_locus_tag="pgaptmp_002712"
     CDS             6333..6521
                     /locus_tag="ACHKNP_RS13560"
                     /old_locus_tag="pgaptmp_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HGU4704851.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
     gene            6628..6909
                     /locus_tag="ACHKNP_RS13565"
                     /old_locus_tag="pgaptmp_002713"
     CDS             6628..6909
                     /locus_tag="ACHKNP_RS13565"
                     /old_locus_tag="pgaptmp_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4704852.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLLEKLKHAAEKIRSQFT"
     gene            6875..7951
                     /locus_tag="ACHKNP_RS13570"
                     /old_locus_tag="pgaptmp_002714"
     CDS             6875..7951
                     /locus_tag="ACHKNP_RS13570"
                     /old_locus_tag="pgaptmp_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4704853.1"
                     /translation="MQRKKYDPNLPKNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRVN
                     QLETIKEKLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPTWFPLAMDLALVTGQRREDITN
                     MRFSEIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGTVVEQCRMVSRGDYLISAGI
                     RKNSPDGSLHPDGLTKKFVAARKLTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(8344..8685)
                     /gene="yebY"
                     /locus_tag="ACHKNP_RS13575"
                     /old_locus_tag="pgaptmp_002715"
     CDS             complement(8344..8685)
                     /gene="yebY"
                     /locus_tag="ACHKNP_RS13575"
                     /old_locus_tag="pgaptmp_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HGU4704854.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(8698..9570)
                     /gene="copD"
                     /locus_tag="ACHKNP_RS13580"
                     /old_locus_tag="pgaptmp_002716"
     CDS             complement(8698..9570)
                     /gene="copD"
                     /locus_tag="ACHKNP_RS13580"
                     /old_locus_tag="pgaptmp_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HGU4704855.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(9574..9948)
                     /gene="yobA"
                     /locus_tag="ACHKNP_RS13585"
                     /old_locus_tag="pgaptmp_002717"
     CDS             complement(9574..9948)
                     /gene="yobA"
                     /locus_tag="ACHKNP_RS13585"
                     /old_locus_tag="pgaptmp_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HGU4704856.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            10087..10317
                     /gene="holE"
                     /locus_tag="ACHKNP_RS13590"
                     /old_locus_tag="pgaptmp_002718"
     CDS             10087..10317
                     /gene="holE"
                     /locus_tag="ACHKNP_RS13590"
                     /old_locus_tag="pgaptmp_002718"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HGU4704857.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            10419..11075
                     /gene="yobB"
                     /locus_tag="ACHKNP_RS13595"
                     /old_locus_tag="pgaptmp_002719"
     CDS             10419..11075
                     /gene="yobB"
                     /locus_tag="ACHKNP_RS13595"
                     /old_locus_tag="pgaptmp_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HGU4704858.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            11099..11761
                     /gene="exoX"
                     /locus_tag="ACHKNP_RS13600"
                     /old_locus_tag="pgaptmp_002720"
     CDS             11099..11761
                     /gene="exoX"
                     /locus_tag="ACHKNP_RS13600"
                     /old_locus_tag="pgaptmp_002720"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HGU4704859.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(11758..13818)
                     /gene="ptrB"
                     /locus_tag="ACHKNP_RS13605"
                     /old_locus_tag="pgaptmp_002721"
     CDS             complement(11758..13818)
                     /gene="ptrB"
                     /locus_tag="ACHKNP_RS13605"
                     /old_locus_tag="pgaptmp_002721"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HGU4704860.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCIDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(14027..14686)
                     /gene="yebE"
                     /locus_tag="ACHKNP_RS13610"
                     /old_locus_tag="pgaptmp_002722"
     CDS             complement(14027..14686)
                     /gene="yebE"
                     /locus_tag="ACHKNP_RS13610"
                     /old_locus_tag="pgaptmp_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU4704861.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDKPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(15013..15369)
                     /gene="yebF"
                     /locus_tag="ACHKNP_RS13615"
                     /old_locus_tag="pgaptmp_002723"
     CDS             complement(15013..15369)
                     /gene="yebF"
                     /locus_tag="ACHKNP_RS13615"
                     /old_locus_tag="pgaptmp_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HGU4704862.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(15436..15726)
                     /gene="yebG"
                     /locus_tag="ACHKNP_RS13620"
                     /old_locus_tag="pgaptmp_002724"
     CDS             complement(15436..15726)
                     /gene="yebG"
                     /locus_tag="ACHKNP_RS13620"
                     /old_locus_tag="pgaptmp_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HGU4704863.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDSADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            15860..17038
                     /gene="purT"
                     /locus_tag="ACHKNP_RS13625"
                     /old_locus_tag="pgaptmp_002725"
     CDS             15860..17038
                     /gene="purT"
                     /locus_tag="ACHKNP_RS13625"
                     /old_locus_tag="pgaptmp_002725"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HGU4704864.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEQLQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGLQIRLFGKPAINGSRRLGVALATAESVVDAIKRAKHAAGQVKVQG
                     "
     gene            complement(17094..17735)
                     /gene="kdgA"
                     /locus_tag="ACHKNP_RS13630"
                     /old_locus_tag="pgaptmp_002726"
                     /gene_synonym="eda"
     CDS             complement(17094..17735)
                     /gene="kdgA"
                     /locus_tag="ACHKNP_RS13630"
                     /old_locus_tag="pgaptmp_002726"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGU4704865.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(17772..19583)
                     /gene="edd"
                     /locus_tag="ACHKNP_RS13635"
                     /old_locus_tag="pgaptmp_002727"
     CDS             complement(17772..19583)
                     /gene="edd"
                     /locus_tag="ACHKNP_RS13635"
                     /old_locus_tag="pgaptmp_002727"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HGU4704866.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(19818..21293)
                     /gene="zwf"
                     /locus_tag="ACHKNP_RS13640"
                     /old_locus_tag="pgaptmp_002728"
     CDS             complement(19818..21293)
                     /gene="zwf"
                     /locus_tag="ACHKNP_RS13640"
                     /old_locus_tag="pgaptmp_002728"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGU4704867.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            21631..22500
                     /gene="hexR"
                     /locus_tag="ACHKNP_RS13645"
                     /old_locus_tag="pgaptmp_002729"
                     /gene_synonym="yebK"
     CDS             21631..22500
                     /gene="hexR"
                     /locus_tag="ACHKNP_RS13645"
                     /old_locus_tag="pgaptmp_002729"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HGU4704868.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            22628..24070
                     /gene="pyk"
                     /locus_tag="ACHKNP_RS13650"
                     /old_locus_tag="pgaptmp_002730"
     CDS             22628..24070
                     /gene="pyk"
                     /locus_tag="ACHKNP_RS13650"
                     /old_locus_tag="pgaptmp_002730"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGU4704869.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(24130..>24732)
                     /locus_tag="ACHKNP_RS13655"
                     /old_locus_tag="pgaptmp_002731"
     CDS             complement(24130..>24732)
                     /locus_tag="ACHKNP_RS13655"
                     /old_locus_tag="pgaptmp_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HGU4704870.1"
                     /translation="EFSWARKIMDYTRGELSYLKIIEQKKEFLHKNNLVIIRCHDVMD
                     REPTFGMSKALAQQLELDVTNIVASDDMYHVYAIEPDSAINITKRFAKNLKIYNLPGI
                     QFYGDRARIVRTIGIGAGCFCDPIQYMEYQADYYITINDSIKTWVQTQYSKDSGLPMA
                     VIGYSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDYDWIE"
BASE COUNT         6681 a         6023 c         5972 g         6056 t
ORIGIN      
        1 tgcaagttta gtgtttaata aaccattaag tcaagtattt gcttgtttag tgattactaa
       61 agataattac cacatgcaaa aaaaagaaat tcgccgttta cgtctcaagg agtggtttaa
      121 agataaaact ctgccaccca aagagaagag ctacctatct caactaatga gtgggagagc
      181 ctcgtttgga gaaaaggctg ccagaagaat agagcaaaca tacgggatgc cggaagggta
      241 tctggatgcg gaatacgcag aacaaccggg ggtttctcca ccacatgcag ggttaacgtc
      301 taatcaactg gaattattgc agattttttc agccttccct gaggatgagc aacgccagat
      361 aatcagcgag ttaaagcaga aaaaagaatc aatggaagat ctcatagcga gatggattgc
      421 ggcgcaaaaa tgccgccgcg cctgagttat aaaaccggag gaaacatgaa tagagccctt
      481 tcaccaatgg tttctgaatt tgaaaccatt gaacaagaaa acagttacaa cgaatggctg
      541 cgtgcgaaag tagcaacgag ccttgcagat ccgcgcccag caattcccca tgacgaagtt
      601 gagcgcagaa tggcagaacg ctttgctaag atgcgcaagg aacggagcaa gcagtaaaat
      661 gttacccgtg ttatggcttg aaagcgcaga taccgaccta gatgatataa ctagttatat
      721 tgctcgtttc gacatagatg cggctgaacg cttatggcag cgattaaggg gttgtgtgct
      781 gccgttatcc gaacatccgt atttataccc accaagcgac agagtacctg gcttgcgtga
      841 gattgtagcc caccctaact atataattct ataccgcgta acaacatcaa gcgttgaagt
      901 agtaaacgtg atccacgcaa gacgccagtt tccctaactt tcactaccaa tagaaacata
      961 acaaccgcaa cgactttatc aaaagcgttg tgtttgttat gtcccgcggt ttagttttta
     1021 cttgacttaa gtttaatgtt tattaaacta aaaataccac cccgccccac agaacgcagg
     1081 gaaatacttc gagttacccg gcagtggtca ggggttaagt agccagcccg aggcgtatga
     1141 acatgacggc agggttcaac tttaataact atgcagcagg tttttgttcc gctaccccgg
     1201 cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacaac gcatctctgg
     1261 tgtacggcgt gtttccaaac caggacggca cgttcaccgc aatgacgtat accaaaagca
     1321 aaacgtttaa aaccgaaaat ggtgcccgtc gctggcttgc caaaaatacc agctaaacca
     1381 tttattggat taattcaata ttcttgctgt aggggtatag ccgaggccac caaagcccgg
     1441 aggtggtgaa ataaaaccgg gcacaacacg aaggcgcatt tccggtatcc ataaagagtc
     1501 ggtcttgtct gttaaattta aatggtggga gtgcgcctcc ggttgtgaat aacaacactg
     1561 ctgtgtgtag tcttggcggc atcagttttt tcttgaagtt cgactgatgt ccgccctttt
     1621 taaagtgaat tttgtgatgc ggtgaatgcg gctaagcgca cgcggcacag ttaaaagtca
     1681 tgttagtcct tattggtttg ggtgggaaag ccgactgtaa ttgttaactg gttgcagtca
     1741 cctggaggca ccaggcaccg catcaacaaa gttcatttgt aaaaatggag ataattatga
     1801 ttgcacatca cttcggaact gatgaaatac cacgtcagtg tgtgactcct ggtgattatg
     1861 ttcttcatga aggccggaca tatatcgcct cggcaaacaa tattaaaaag cgaaaacttt
     1921 atattcgtaa cctgactaca aaaacatgca tttctgactg catgattaaa gtcttcctcg
     1981 gtcgtgatgg tttacctgta aaggcggagt catggtgatg actaagaaaa taaaatgtgc
     2041 ttaccacctt tgcaataaag gaattgaaga aagcaaaagc attaaaagac cacttcattt
     2101 catgcgtgga gttatcccaa cgacggaaat gaaaaaatat tgtagtgaaa attgtgccga
     2161 aaaagaccag atggcacacg aactttaatt aactgactat gcgaaactga atttatgcca
     2221 gcaatggcag ggattcgctc aaccttaatt aaggagaaaa aatgattacc aattatgaag
     2281 tcactgttgt aactaccgat gacattgttc acgaggttaa tctggaagga aagcgtattg
     2341 gctacgtgat taaaacagaa aataaagaaa ccccattcac tgtggttgat attgacggtc
     2401 catcaggcaa cgtaaaaaca cttgatgaag gtgtcacaaa aatgagtctg gttcacatcg
     2461 gaaagaatct gcccgcagaa aaaaaagccg gatttctggc aactctgatt gcaatgaaat
     2521 taaaaggtga aatctgaaaa aagaaagcct gcacaacgtg caggcctgag tgaagaacct
     2581 gggacattta ttcatcactc gcagtaattt taatctgagt tgaggttaaa aaacaatgag
     2641 cacaaaacca ctcttccttt tacggaaagc gaaaaaatca tccggtgaac ctgacgtcgt
     2701 cctgtgggca agcgacgatt ttgaatcaac ctgtgccact ctggactacc tgatcgttaa
     2761 gtcaggtaaa aaactgagca actattttaa agctgttgcc acaaattttc ctgtcgttaa
     2821 tgaccttccc cctgaaggtg agatcgattt tacctggagt gaacgctatc aactcagcaa
     2881 agactccatg acctgggaac taaaaccggg agcagcgcca gacgacgttc accatcagga
     2941 ggatgcaccg gaaaccgaag aaccgacggg aggccaggaa gaaaacgcgc aggcagacgc
     3001 ccacgaggat tgccaggatt gcgaagtctc tgtagccact ttgcggttca cacagcgtct
     3061 tctgcacatt tttacgtatg cagccgggga tcgaaaatac ctgcatcatg ccacccgtga
     3121 acaacgcaaa cacattactg ctcttgagat ggatcaggaa aacagctatg tccagaatct
     3181 gctgttggcc atacgcagca tggcagaacc gacaactctg gataatgccg ccctgctccg
     3241 cctgactgat gcaattaagg cagtgttctc tatcacgaaa aaacatcagc cctatgaatt
     3301 taagaatttc atttcagcct ggctggatac cgaacacatt gatcgcggtc ttctgacaaa
     3361 agaatggcga aaagggaatc gtgtttcacg catcactcgc acggcttccg gtgctaatgc
     3421 tggcggcggg aacctcaccg atcgcggcga aggtttcgtc cacgatctga catcactggc
     3481 gcgcgatgta gccactggcg tactggcccg ttcaatggac gtggacatct ataaccttca
     3541 tccggcacac gctaaacgca ttgaggaaat tatcgctgaa aataaaccac ccttttctgt
     3601 tttccgcgac aaattcatca ccatgcctgg cgggctggat tattcccgcg ccatcgtggt
     3661 tgcgtccgtg aaagaagcac caattgggat cgaggtcatc cccgcacacg tcactgaata
     3721 tctgaacaaa gtactgactg aaaccgatca tgccaaccct gatccggaaa tcgtggatat
     3781 tgcctgcggt cgctcctcgg ccccgatgcc gcaacgagta acagaagaag gaaaacagga
     3841 cgatgaagaa aaaccacaac catctggcgc aatggcagat gaacaggcaa cggctgaaac
     3901 agtggaaccg aatgcaactg aacatcatca gaacacgcag ccgctggatg ctcagtcaca
     3961 ggtaaattct gttgatgcga aatatcagga actgcgggca gaactccatg aagcccggaa
     4021 aaacattcca tcaaaaaatc ctgtcgatgc cgataaattg cttgctgcat cacgtggtga
     4081 atttgttgac ggaattagcg acccgaacga tccgaaatgg gttaagggga tccagactcg
     4141 cgattctgtg taccagaacc agccagaaac ggaaaaaatc agcccggatg cgaaacaacc
     4201 tgagccagta gtgcaacagg aaccggaaat agtctgcaat gcctgcggtc agactggcgg
     4261 ggataactgc cctgactgtg gtgcggtgat gggcgacgca acataccagg aaacattcgg
     4321 tgaagagaat caggttgaag ctaaggaaaa agatccggag gaaatggaag gcgctgaaca
     4381 tccgcacaat gagaatgctg gcagcgatcc gcatcgcgat tgcagtgatg aaactggcga
     4441 agtcgcagat cccgtaatcg tagaagacat agagccaggt atttattacg gaatttcgaa
     4501 tgagaattac cacgcgggtc ccggcgtcag taagtctcag ctcgatgaca ttgctgatac
     4561 tccggcactg tatttgtggc gtaaaaatgc ccccgtggac actacaaaga caaaaacgct
     4621 cgatttagga accgctttcc actgccgggt acttgaaccg gaagaattca gtaaccgctt
     4681 tatcgtagca cctgaattta accgccgtac aaacgccgga aaagaagaag agaaagcgtt
     4741 tctgatggaa tgcgcaagca caggaaaaac ggttatcact gcggaagaag gccggaaaat
     4801 tgaactcatg tatcaacgcg ttatggcttt gccgctgggg caatggcttg ttgaaagcgc
     4861 cggacacgct gaatcatcaa tttactggga agatccggaa acaggaattt tgtgtcggtg
     4921 ccgtccggac aaaattattc ctgaatttca ctggatcatg gacgtgaaaa ccacagcgga
     4981 tattcaacga ttcaaaacgg cttattacga ctaccgctat cacgttcagg atgcattcta
     5041 cagtgacggt tatgaagcac agtttggcgt gctgccaact ttcgtttttc tggttgccag
     5101 cacaactgtt gaatgcggac gttacccggt tgagattttc atgatgggcg aagaagcaaa
     5161 actggcaggc cagcaggaat atcaccgcaa tctgaggacc ctggctgact gcctgaatac
     5221 cgatgaatgg ccagctatta aaacgttatc actgccccgc tgggctaagg agtatgcaaa
     5281 tgactaagca accacctatc gcaaaagccg atctgcaaaa aacccaggga aaccgtgcac
     5341 cagcagcagt aaaaaataac gacgtgatca gctttattaa tcagccatca atgaaagagc
     5401 aactggcagc agctctccca cgccatatga cggctgaacg aatgatacgt atcgccacca
     5461 cagaaattcg taaggttccg gcgctaggaa actgtgacac catgagtttt gtcagtgcga
     5521 tcgttcagtg ttcacagctc ggccttgagc caggtagcgc cctcggccac gcatatttac
     5581 tgccttttgg taataaaaac gaaaagagcg gtaaaaagaa cgttcagcta atcattggtt
     5641 atcgcggcat gattgatctg gctcgccgtt ctggtcaaat cgccagcctg tcagcccgtg
     5701 ttgtccgtga aggtgacgag tttagttttg aatttggcct tgatgaaaag ttaatacacc
     5761 gcccgggaga aaacgaagat gcaccagtga cccacgtcta tgctgtcgca agactgaaag
     5821 acggagggac tcagtttgaa gttatgacgc gcaaacagat tgaactggtg cgcagccaga
     5881 gtaaggctgg taataacggg ccatgggtaa ctcactggga agaaatggca aaaaaaacgg
     5941 ctattcgtcg cctgtttaaa tacctgcctg tctcaattga aatccagcgt gcagtatcaa
     6001 tggatgaaaa ggaaccactg acaatcgatc cggcagactc ctctgtatta accggggaat
     6061 acagtgtaat cgataattca gaagaataat tcagcctggc ggtgtaatgc accgccaacg
     6121 tgagacagtt tttatgacaa aaattatgag atatgacgat gttaaaccat gtccgttttg
     6181 tggttgtcca tctgttacag tgaaagcaat ttcaggatat taccgggcaa aatgcaacgg
     6241 atgcgaatcc cgaactggct atggtggaag tgaaaaagaa gcactcgaaa gatggaataa
     6301 acgaactact ggaaataata atggaggtgt tcatgtataa aataactgcc actattgaaa
     6361 aagaaggtgg cactcctact aactggacaa gatattcaaa atctaaatta acgaaatcag
     6421 aatgcgaaaa aatgctctca gggaaaaaag aagcaggcgt gtccagagag cagaaagtaa
     6481 agctgataaa ttttaattgc gagaaacttc tgtcctcgtg agttgcatta tatacaaatt
     6541 agaacttcat agctgattat taaaaatcaa ccacacccgc cagtattctg tatatttact
     6601 ggcggtcata tcgtaagagg tatggcaatg aatcttgtga cactcaaaac gtggggaaaa
     6661 ctcagatatc cggataaccc accatcaata tcaacgctga gacgatgggc aaggaatgga
     6721 aacatttatc ctgcacctga actacacggg aggagttaca gggtggttcc ggaagctttc
     6781 tatatcaacc caaataaggt tgataccgat ataacacacc atcagcctaa tgggcgacaa
     6841 gggagagaca gtccgttact ggagaagtta aaacatgcag cggaaaaaat acgatcccaa
     6901 tttacctaaa aacttaacat atcgaaggag ggacaaagca tattactggc gcaaccctct
     6961 gacgaaagaa gaatttacac taggtaaaat ttcaagaaga gatgcagtag cgcaggcaat
     7021 tgaagcaaat cattatatat acaaaaacta ctctcctgct gccttaattg aaaaactaaa
     7081 agggttcgac tcatttacta tggcagactg gattgaacgt tacaaaacga ttcttataag
     7141 gagaaaagtg tccagaaata cttataaaat tcgggtaaat caactggaga caataaaaga
     7201 aaaattagga ggggttttac tgacagaaat aaccactcgc catattgccg agtttcttga
     7261 tttgtggatt gaaggaggga aaaacacgat ggcaggatca atgcgttctg tgttatctga
     7321 tatgttccgc gaggccattg ttgaaggacg tatatctcaa aatccagtaa cgccaacaag
     7381 agcaccgaaa atagtagtta caagagaacg gctgaaacta aagacataca actgcatcag
     7441 ggaggcagca gatcaacttc cgacatggtt cccattagct atggacttag cccttgtaac
     7501 aggacaacgt cgcgaagaca taacaaatat gcggttcagt gagatttatg atgatcgtct
     7561 ccacatcagg caaattaaga caggaatgat gattgctatc cccctgtcac tcagccttcc
     7621 tgtcgctggt ttacgacttg gtacagtagt tgaacagtgc cgcatggtaa gtagggggga
     7681 ttatctaatc agtgccggga ttagaaaaaa cagccctgac ggcagccttc acccggacgg
     7741 cctgacaaag aaatttgtcg cagccagaaa attaacaggt atccagttca gtgaaaaccc
     7801 accaactttt cacgagatca gaagcctggc tggacgattg tacaaagaaa catgtgggga
     7861 agaatttgct caacgtctac ttggccacac atcggagaag acaacaaaaa tgtatcttga
     7921 tgagagagaa aaaacgtact tactgctctg attttaacgt aaatggattg ttaaatgtgt
     7981 tttggttgtg atataaccaa aaaagaccgg aatacagaaa ttcgagtaaa tttcgagtaa
     8041 tttcggggag acgtttgcaa ctaattgatt ttaaatacaa ttaaaaaaag accgaatacg
     8101 attcctgtat tcggtccagg gaaatggctc ttgggagaga gccgtgcgct aaaagttggc
     8161 attaatgcag gcttagttgc cttgcccttt aagaatagat gacgacgcca ggttttccag
     8221 tttgcgtgca aaatggtcaa taaaaagcgt ggtggtcatc agctgaaatg ttaaaaaccg
     8281 cccgttctgg tgaaagaact gaggcggttt ttttattgga aatcaaaagg ctattttagg
     8341 taattaacag agtttttcag ctcgttctat aaacggtgcc agactcattt tttcgccggg
     8401 attgttagga tcatcaatct gaatcaccga aatgggttgg gctttagtct tcccactggc
     8461 aacttccttt tgtgcgatat cgtttaaagg atactgcacg agggtacttg ggttaatgac
     8521 atacaaagca ttacccggtc tgcaagtcag catcacctct tcgcgattaa acgcccattt
     8581 gtctttaccc acttcaaaac gactgacggt aatcacctgc ggtgcagcca gcgccgctgc
     8641 agaactggtg agtaacagaa acgtcagaat actttttttc atcattttcg taatccatca
     8701 aaaaggttcg agagtcgcaa gcaggctgat gatcaccagc actaccgcac ctatcgccca
     8761 ttcgagttta gtcatccact caaaccaggg tgccgcacga tcttcatctt gcctcatgcg
     8821 cggtacaaga acataacgat tagccagcgc aatgaccacc ataatcatca ccaggatggc
     8881 tttgagcaat aagagttggc cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa
     8941 caatgcatta agcacgccgc ttgccagtac gccgatcacc gcaaaatgcc cgcaccagga
     9001 aaaacgcatc agcgcctgaa tggcctggtg tcgccaacgg cctttgatga gttgcataca
     9061 ccagagcacc ggcagtaatc ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg
     9121 attagtctga tggattttcg ccgttacccc ttcattcagt gtcgcatgcc cgattcccgc
     9181 cagcagaata aattgcgcgg tagtgagcat aaacagcaaa cgtggcatat tccgcggttg
     9241 cataagggcg acgatcaacg ttacgagggc gagaacaatt tgccataacc agacgccacc
     9301 aaactgcgtt tgcaaaacag cctgccaaat gtttggcgaa aatacatctg cccatcccgt
     9361 tcccatcaac ccgccctgaa cggcgagcat tgcagtggca ctgatcaaac tccagacggc
     9421 agcgtgctgt tgtaaacgca gaaaacgctt cgccagcaag cgacgaatcg ttaagggggc
     9481 cagccaggca ccgtacattg cgaagccaaa gaccagcatc agcgaggtga aatggataaa
     9541 tcgcagcgcg atccaggtaa acgccagcat gatttatttc acgctaaagg tgtagtgtcc
     9601 tttcgtttta tgcccgtcca ccgaaacaac atgccagtca acgatgtagg ttccgggttt
     9661 cagcgaatcg gccagtggaa caatcagttg cttttggtcc tgctcatttc gcttcgccgg
     9721 taatgtttta atattttcgt tttttggccc cgtgattttt gcaccactga atcctgtttc
     9781 aacaccttcc gagaagttta aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc
     9841 cgcaggatac tgatgcgtta aatgcgcatg tgcccagacg gaaggtgtca ccaacgaagt
     9901 ggtcaggatt gccagcgcgt agcgaaggga gcgtgcagtt gaagtcatat tatctattcc
     9961 tttttgtaat aactttttta cagagcataa ccttgtctaa tgtctgagtc gaggatcatc
    10021 aattccggct tgccatcctg gctcactctt agtaactttt gcccgcgaat gatgaggaga
    10081 ttaagaatgc tgaagaatct ggctaaactg gatcaaacag aaatggataa agtgaatgtc
    10141 gatttggcgg cggccggggt ggcatttaaa gaacgctaca atatgccggt gatcgctgaa
    10201 gcggttgaac gtgaacagcc tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc
    10261 caccgtttgg cttcggtcaa tctgtcacgt ttaccttacg agcccaaact taaataaaac
    10321 ttatacagag ttacactttc ttacataacg cctgctaaat tatgagtatt ttctaaaccg
    10381 cacccataat ttgcagtcat tttgaaaagg aagtcattat gtcgttctgg aaagttgcag
    10441 cagcgcaata tgagcctcgt aaaacctcac tcaccgaaca ggtagctcat catctggagt
    10501 ttgtgagggc cgccgccagg caacaatgtc agctgttagt ttttccatcg ctttctttac
    10561 tggggtgtga ttattcgcga cgtgcccttc ctgccccacc cgatctctca ctgttagacc
    10621 cgctttgtta tgccgcaacg acctggcgaa tgaccatcat tgccggcctt cccgttgaat
    10681 ataacgatcg ctttattcgt ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga
    10741 tctaccatca gagtcatggt gcatgtctgg gtcgtcgttc cagaaccatc acggtggtcg
    10801 atgaacaacc gcaaggtatg gatatggacc cgacctgttc actgtttact acagggcaat
    10861 gtctgggaga acccgacctg ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt
    10921 actcgattgc cgtgctgatg gcaaatgccc gtggtaacag tgcactatgg gatgaatatg
    10981 gtcgtctcat cgttcgcgcc gatcgtggtt cattattgtt agtcggtcag cgttcatcac
    11041 agggttggca aggcgatatc attccattac gctaggcttt ttcggcctgg agcatgccat
    11101 gttgcgcatt atcgatacag aaacctgcgg tttgcaggga gggatcgttg agattgcctc
    11161 tgttgatgtc attgacggaa aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg
    11221 tcctattagt ccacaagcga tggcgattca tcgcatcacc gaagccatgg tcgccgataa
    11281 accgtggatt gaagatgtga tcccacacta ttacggtagt gaatggtatg tcgcgcataa
    11341 cgccagcttt gaccgccgcg tactgcctga gatgcccggt gagtggattt gcactatgaa
    11401 actggcccgt cgtttgtggc ctgggatcaa gtacagcaat atggcgttat ataaaacacg
    11461 caagctcaat gtacagacgc cgccgggcct gcatcatcac cgcgcgttgt atgactgtta
    11521 tatcaccgcc gcgttgctta tcgatattat gaacacctcc ggctggacgg cagaacagat
    11581 ggccgatatc accggacgtc cgtcgttgat gacgaccttc acctttggca aataccgtgg
    11641 caaagcggtt tccgacgttg ccgaacgcga tccgggctat ctgcgctggt tatttaataa
    11701 cctggacagc atgagcccgg agctgcgttt aacactgaaa cattatctgg aaaatactta
    11761 gtccgcaggc gtagcgggta atgttccctg cgccagcgcg accagaaaag catattccat
    11821 cgctacgcct tcgtacgatt taaagcgacc agatttaccg ccatggcctg agtccatgtc
    11881 gatacagagc aataaaagat ggtcatcggt tttcagctcg cgcaatttag cgacccattt
    11941 tgccggttcc caatattgca cctgagaatc gtgcaaaccg gtcgttacca gtaaatgcgg
    12001 ataagcctgt gcggtgacgt tgtcatacgg gctgtagctt ttcatgtact cgtaatattg
    12061 cggatcctgc ggattacccc actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc
    12121 aagcatcgtt gttacaacat caacaaacgg tacctgggcg ataacgccgt ggaataattc
    12181 cgggcgttga ttaattgcaa cgcccatcaa catgcccccc gcactcccgc ccatcgcata
    12241 acaaagcgaa ggagagccat agcccagttt taacaatgca tcgcaggcat caagataatc
    12301 attaaacgta tttttcttct tcagaaattt tccgtcttcg taccattgtt gccccagctc
    12361 accaccgccg cgaacatgga caatggcgta gacaaagcca cgatctaaca aactcaagcg
    12421 gctaaaactg aaatcggcat caatacttgc gccgtaagaa ccatagccat acaccagcaa
    12481 cgggttgtgt cctttgcgaa aatgtttgcg atggtagacc aacgaaaccg gaacttcgac
    12541 gccatcacgg gcgactatcc acaggtgttc actgcggtaa ttcgccgcat caaaaccggg
    12601 cacttctgtt tgttttaata cacgacgctc accggtatcc atatccagtt caaacaaagt
    12661 gtctggtgtg gtcatggaag aataaccata acgcaatcgc gcggtttcag gttctggatt
    12721 gtaggcaatc caggtcacat aggctggatc atcaaaggca ataccaatga cttcccgggt
    12781 cttgcggtta atttggcgca aactggttaa cccgcgctga cgctcttcaa ccaccagcca
    12841 gtcggtaaac agcgtaaacc cttccagcat gatgttttcg cgtggcggaa ttaactcttc
    12901 ccactgttgc tcatcacgca tacgggtacg gtataagcca aagtttttgc cgtggcggtt
    12961 ggaacgtaga taaaaacgat gctggtagtg atcaaggctg tattcgtgat ctttgcggcg
    13021 cggcagaaaa acaaacggct cggcatcggc catttccgcg tccagcaggc gaacttcact
    13081 ggtggtggcg ctggccaaat gaatgactac atagtgcttc gaggtcgttt tatgcaggct
    13141 gacgtaatag gtatcgtctt tttcttcgta gatcagttta tcttgcgatg ctggcgtacc
    13201 gatggcgtga cgccagacct gataaggcag cagcgtcacc ggatgcttgc gaacatagta
    13261 gaaagtccag gagtcatttg cccagacaaa gctgggttca acgttatcca gcagttccgg
    13321 gtaccagtta ccagtttcca gattacgaaa acgaatgccg tactggcgtc gggaaagaaa
    13381 atcttctgcc agcgccataa tggtgttatc gggcgtaatc gccattccgc ccatcgaata
    13441 aaactcacta tgagctgcgc gcttattggc atcgagcaat gtttcccact catcccactc
    13501 ttcactgaat gccgattgac gctggtagat agcatattca cagcctggtt cataaatatg
    13561 ccgatagcgg tagccatttt tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc
    13621 gatgatttcc tttaagatgc gatcctgcaa ggcttgttgt gaggccatca cccgatgacc
    13681 gtaactattt tcttgttgca ggtagtccag gacttctggc tgagaacgcg tatcgtcccg
    13741 cagccagtag taattatcga tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg
    13801 ggcggctttt ggtagcatgt tattgttctt tctggttgaa acatcttata agggtggcaa
    13861 aactcaccgg ggatgcaagc gaaacagggg agtcattgct tagatgatga caggtaatgg
    13921 cgcggatatc gaatgttatg cagacagaga aaatcagcct gttcaaatgg ctgtgcgatt
    13981 ctggatagcc cgaaatagtc aacttcaggc tatccagaga gcggaattat tccgccaaag
    14041 tgcgtttttg ctgttcgaga tcgcgttcaa tgccgtcacg aacatcctgg gggattttca
    14101 gcgcgtcacc cagtgcattc aggtaactgc gttccataaa atggtcaata tcaatagccg
    14161 cgcaactcag gaaatagatt tccagcgcct cttcttcatt gcggaccccg gtagccaggc
    14221 gttgtggatc cagcggttgt tcgattgcct gctcaatgag tacacgcccc tgctcttcca
    14281 cgccggcttc acgcaattgc tggtcgattg ccgcacgttc tttggcatca atatgaccat
    14341 cacttttagc ggcaaagacc agcgcaagga tcaaacgttc tgtacgctca tccagcggcg
    14401 tactttgcgc gccaaactgc ggtttgtcct gatgcgccgc gcgaatttta tctttgtatt
    14461 tattccacag caccgtaccc gctaccgctc cgccgccaac cagtaacgcg ttggtgccat
    14521 atttcgtaag aagtttacgt gctgatttat ttgcgaccag cagcccagcc agtccgccta
    14581 atgcgcctgg cactaacagt ttgcccaatc cctgatccgc agacgaggag gtagaagaac
    14641 tgctttgccc aagcagggat tgcagttgat ttaaccagtt agccatattt gctcctcaat
    14701 aaccattttt agtgcctgta gcatagcgga tgaggatgtc agaaaatgta tatcgagaca
    14761 aaagatgcgc aaataagcgg ggcggtaggc cgttattcaa agaaattcgc gccaggtaag
    14821 gtatttactg tatatcaggt gataagggta ttttaggtga aagtagtttg tcgttttgag
    14881 tggcattgtt tgatgctgat tgcccgatgc gacgctgttg cgtcttctca ggcctacgat
    14941 tgtggtgcgt tctggggcgg acaaggcact cgcgccgcat ccgccaacgg tcaatcgaat
    15001 tatcagaagc cattaacgcc gctgatattc cgccattccc gctttgcagt ccacgctgac
    15061 ctggtaatga atatcggcac ttttaccacg cacggttagc ggtactgacc atttatcatc
    15121 tttaccctga atgtcctgca aactgaccca agccacggga tcggcctgac cgacaatttt
    15181 ttgatcatct gcccaacgcg ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc
    15241 aattccggca gcatccagac cttcacactt tgggaaagtg accgacttgc tggtttcatt
    15301 attggcagcg aaaactgatg cgcaggcaga aaccaacaac agccctaaaa acgcccctct
    15361 ttttttcatg tttttctcca tagcacaatg attcaggaga aagcatggta caaattgtca
    15421 ggagcgcaag ttgcttcagg ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac
    15481 gacctgtggg gacggtaatt tgccggtttt cagaatggtg ctcagcacat ctttttgttc
    15541 tgccagccat agcgaaaggg cttcacgttg ctcgtcttcc atttgcactg gagaatttgt
    15601 cagccaggtg tcaagcagat ccgccgaatc aagcatttta tcataagcat cggcttcctt
    15661 tttgctggta aacgacattt tctcttcgcc ctcacgaatg actacgtatt taacttcaac
    15721 cgccatttgc agcctctcat aataactgtg attttataca gtatatttct tttcggttga
    15781 gaaatcaaca tcagcaataa agacacacgc aaacgttttc gtttatactg cgcgcggaat
    15841 taatcagggg atattcgtta tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg
    15901 cgtgatgtta ttaggctccg gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct
    15961 cggcgtagag gtgattgccg tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca
    16021 tcgctcccat gtcattaata tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga
    16081 aaaaccacat tatatcgtgc cggagatcga agctattgcc accgatatgc tgatccaact
    16141 tgaagaggaa ggactgaatg ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg
    16201 cgagggtatc cgtcgcctgg cggcagaaca gctgcagctg cccacttcca cttatcgttt
    16261 tgccgatagc gaaagccttt tccgcgaggc ggttgccgcc attggctatc cctgcattgt
    16321 aaaaccggtg atgagctctt ccggcaaggg gcagacgttt attcgctctg cagagcaact
    16381 tgctcaggca tgggaatacg ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt
    16441 tgaaggcgtc gttaagtttg acttcgaaat taccctgcta accgtcagcg cggtggatgg
    16501 cgtccatttc tgtgcaccag taggtcatcg ccaggaagat ggcgactacc gtgaatcctg
    16561 gcaaccacag caaatgagcc cgcttgccct tgaacgtgcg caggagattg cccgtaaagt
    16621 ggtgctggca ctgggcggtt atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga
    16681 ggtgattttc agtgaggtct cccctcgtcc acatgatacc gggatggtga cgttaatttc
    16741 tcaagatctc tcagagtttg ccctgcatgt acgtgccttc ctcggacttc cggttggcgg
    16801 gatccgccag tatggtcctg cagcttctgc cgttatcctg ccacaactga ccagtcagaa
    16861 tgtcacgttt gataatgtgc agaatgccgt aggcgcaggt ttgcagattc gtttatttgg
    16921 taagccggca attaatggca gccgtcgtct gggggtggca ctggctactg cagagagtgt
    16981 tgttgacgcc attaaacgcg cgaagcacgc cgccggacag gtaaaagtac agggttaaac
    17041 ccggcaaaaa aacgctacaa aaatgcccga tccgcgatcg ggcattttga cttttacagc
    17101 ttagcgcctt ctacagcttc acgcgccagc ttagtaatac ggtcgtaatc gcccgcttcc
    17161 agcgcatctg ccggaaccag ccaggaacca ccgatgcaca gcacgctttt cagcgccagg
    17221 tagtcacggt agttagccgg agaaatacca cctgtcgggc agaaacggac ctgggagaac
    17281 ggacccgcga tcgcctgcag ggctttcacg ccgccgttag cttcagccgg gaagaatttg
    17341 aactctttca aaccgtagtc catacccagc atcagttcgg aaacagtgct gatccccgga
    17401 atcagaggaa tagtcccttc ggtagcagct ttcagcagcg gctcggtcag acccgggcta
    17461 attgcgaact gtgcacccgc ttcggtgact tctgccagct gctgtggatt cagcaccgta
    17521 ccggcaccca caatcgcttc aggcacttct ttggcgatag cacggatagc gtcaactgca
    17581 cactcggtac gcagagtcac ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc
    17641 atcggcactg cgtgttccag tttttttacc acgataaccg gtacaaccgg gccggtggtc
    17701 aggattgatt ctgcacttgt tttccagttt ttcatcagag ttttctctcg cctgattaca
    17761 aatttgtcgt cttaaaaagt gatacaggtt gcgccctgtt cggcaccgga cagtttttca
    17821 cgcaaggcgc tgaataattc acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg
    17881 tgcggttcgc gagcagccag ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt
    17941 ccattcacac gaatgatgtc cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct
    18001 tctggtgtta cgtggatagc tgacggcact ttacctgaag cgccggagag tcgtccatcg
    18061 gtaactaacg caattttgaa acaccggtcc aataatacac caagtggcgg catgagttta
    18121 tgtaattctg gcattccgtt cgcttttggc ccctgatgac ggacaacaac gacacaatcg
    18181 cggtccagca aacccgcttc aaaggccggc ataacgtcat gctggctttc aaaaacaacc
    18241 gctggcgctt caatcacctg gttctcaacc ggcacggcag aggttttcat aaccgcacgg
    18301 cccaggttac cgcttaacac ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa
    18361 gcgatcacat tgctgtcgag tgatttttcc gccccttccc gccagtccag ttcaccatta
    18421 ttcagccatg gttcaagggt ataacgagac agaccaaagc ctgccaccgt attgacatct
    18481 tcatgcagca ggcctgcttt gagcagttca cgcaccagaa ccggtacgcc acctgccgcc
    18541 tggaagtggt taatatcggc cggaccgttc gggtagagac gtgccatcag cggtacaaca
    18601 tcagaaaggt cagagaagtc atcccagtta atctgaatac cggccgcgcg cgccatcgcc
    18661 accaggtgca tggtgtggtt agtggaacca ccggtcgcca gcagtgcaac gataccgttc
    18721 accaccactt tctcatcgat catcttaccg atcggcatcc attcattacc attaccggtc
    18781 atgcgtgtaa cctgacgcgc agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga
    18841 tgaacaaaag aagagcctgg caactgcatc cccataaatt ccaccaccat ctggttggtg
    18901 ttggcagtac cgtagaaagt acatgttccc ggcgcatggt aagacgcggc ttctgactcc
    18961 agtaaggcca tgcggtccac tttaccttcg gcataaagct ggcgaatacg cactttttct
    19021 ttatttggca aaccgcttgc catcggtcca gacggcacaa acaccgcagg caaatgacca
    19081 aacgacaggg ctgccatcgt cagacccggg acaatcttgt cgcacacacc gaggaacaga
    19141 gcaccatcaa acatgttatg ggacagcccg accgccgcag acatcgctat cacttcgcgg
    19201 cttagcagcg acaattccat tccatcctgc ccctgggtga caccatcaca catcgccgga
    19261 acaccgcccg caacctgacc aaccgcattc gcttcatgca gggctttacg aatgatttct
    19321 ggatagtgtt cataaggctg gtgcgcggag agcatgtcgt tataggaggt gatgatggcg
    19381 atattgttac gcaacatgct tttcaaagag gctttgtctt ctggctggca ggcagcgaaa
    19441 ccgtgtgcca ggttaccgca tgccaactgc gaacgatgaa cggtcgaagt tttcgcttgt
    19501 tctatccggg cgagataagc agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt
    19561 acgcgtaaca attgtggatt cataaaggct cctgaaattg agttgtcaga gcaggatgat
    19621 tcacaacgcg tttcattcag aggatttatg actgaaacgc ctgtaaccgg agctcatagg
    19681 gcaaaaacgt ttcagtcagt gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct
    19741 gaaagtgtaa aaattgttct acaatctgtg caagatcatg ttaccggtaa aataaccata
    19801 aaggataagc gcagatatta ctcaaactca ttccaggaac gaccatcacg ggtaatcatc
    19861 gccaccgagg caacgggtcc ccaggttccg gcctgatacg gtttcggcgc atcattgtcc
    19921 atcgcccacg cctcagtaat ggagtctacc catttccagg cttcttccac ttcgtcgcga
    19981 cgaacaaaca gtgcctgaat accacgcatg gtttccagca gcaaacgttc ataggcatcc
    20041 gccagatgcg tctgattaaa ggtttctgaa tagctcagat ccagcttggt gatttgcagg
    20101 ttatgtttgt ggtcaaggcc aggaacttta ttcagtacct ggatatccac gccttcatca
    20161 ggttgcagac ggatagtcag tttattctgc ggcagatcct gccacgattc tttaaacaga
    20221 ttcagttcag gtgttttgaa atagaccacg acttcagaac atttggtcgg cagacgttta
    20281 ccagtacgca ggtagaatgg cacaccggcc cagcgccagt tatcaatgtc gacgcggatc
    20341 gctacgaagg tttctgtatt gctgctcttg ttcgcgccct cttcttccag atatcccggc
    20401 acttttttgc cctgggcgaa gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg
    20461 ttggagcggt cgatgcggcg cagagacttc agtactttca ctttttcatc gcggatgctg
    20521 tctgcgctca ggtcagacgg cggagacatc gcaatcatgc agagaatttg cagcaggtgg
    20581 ttttggatca tatcgcgcat ctgaccggct ttatcaaaat agccccagcg cccttcgatc
    20641 cccacttctt ctgccacggt aatctcaaca tgatcaatgg tgcgattgtc ccagttattc
    20701 acaaacaggg agttagcaaa acgcagcgcc aacaggttca gcaccgtttc tttaccaaga
    20761 tagtggtcga tacggtaaac ctggcactcc tcgaagtatt cgccaacctg atcattgatt
    20821 tcctgcgagg tcgccagcga cgtccccagc ggtttctcca tgactacgcg tgccggttta
    20881 gcattcagtt tagcctcgcc aagccctttg caaattgcgc caaaagtgct gggcggcatg
    20941 gcaaagtagt taatggtgat acgatttttt tgatccagca tcgcgccgag acggctgaat
    21001 gcagcagtgt cattgacatc gagattacaa aaatccagac gtgcactcag ggtgtcccat
    21061 aaaccttcat caatggtttc tttcatgaaa gtttcgagcg cctcgcggac aactttggta
    21121 tatgccgctt tatcccagtc agcacgccct acgccgataa tccgggtgtc cgggttgagc
    21181 tgaccggctt tttccagttg atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct
    21241 ttcgcgccga aaatgaccag gtcacaggcc tgggctgttt gcgttaccgc catgtcattc
    21301 tccttaagtt aactaacccg gtacttaagc cagggtatac ttgtaatttt cttacggtgc
    21361 actgtactgc ttttacgagc ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg
    21421 gtaaaagcgc gcaaagtgcg gcaaaactga taaaaaaatc atcgttttcc attgggtaaa
    21481 aatctgacac tgatcatgtt atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt
    21541 aacacagcac acaggcgtaa tatctgacaa aactacattt cgatttcttt cagtgcggaa
    21601 atcgtcatta cccgtgagtt tctttacatc atgaatatgc tggaaaaaat ccagtctcag
    21661 ctggaacatt tgagcaaatc agagcgcaaa gttgccgagg tcattctggc ttcgcccgat
    21721 aacgcgatcc attcgagtat tgctgctctg gcgctggaag ccaatgttag cgaaccgacg
    21781 gtgaatcgtt tctgtcgcag catggacacg cgcggttttc ctgattttaa acttcatctg
    21841 gcacaaagtc tggcgaatgg cactccctat gttaatcgca atgtcaatga agatgacagc
    21901 gttgaatcat acacagggaa aatatttgag tccgcaatgg caacgcttga tcatgtccgt
    21961 cattcactgg ataaatctgc catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa
    22021 atcgcctttt tcggattagg ctcttcagcc gccgttgccc acgatgcgat gaataagttc
    22081 tttcgtttta atgttccggt ggtgtactcc gatgatatcg tgctgcaacg catgagttgt
    22141 atgaattgta gcgacggaga cgtggtggtg ctgatttctc acactggaag aacaaaaaat
    22201 ctggtcgagc tggcgcagct ggctcgcgaa aacgacgcca tggtgattgc cctcacctct
    22261 gcgggtaccc cgctcgcccg ggaagcaacg ctggcaatta ccctcgacgt accggaagat
    22321 actgacattt atatgcccat ggtttctcga cttgcacagc tgaccgtgat agatgtgctg
    22381 gcgacaggat ttactttgcg acgcggtgca aaattcagag ataacttgaa gcgggtcaag
    22441 gaagcgctga aggaatcgcg ttttgataag cagttactta atttaagtga cgatcgctaa
    22501 aaacgactgt cactgtccta atcttatacg acatccgaat gagattaatt tatcgccatc
    22561 gcggcgttat ttcattcgga tttcatgttc aagcaacacc tggttgtttc agtcaacgga
    22621 gtattacatg tccagaaggc ttcgcagaac aaaaatcgtt accacgttag gcccagcaac
    22681 agatcgcgat aataatcttg aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa
    22741 cttttctcac ggctcgcctg aagatcacaa aatgcgcgcg gataaagttc gtgagattgc
    22801 cgcaaaactg gggcgtcatg tggctattct gggtgacctc caggggccca aaatccgtgt
    22861 atccaccttt aaagaaggca aagttttcct caatattggg gataaattcc tgctcgacgc
    22921 caacctgggt aaaggtgaag gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc
    22981 tgctgacgtt gtgcctggcg acatcctgct gctggacgat ggtcgcgttc agttaaaagt
    23041 actggaagtt cagggcatga aagtgttcac cgaagtgacc gtcggtggtc ccctctccaa
    23101 caataaaggt atcaacaaac ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga
    23161 caaagcagac attaagactg cggcgttgat tggcgtagat tacctggctg tctccttccc
    23221 acgctgcggc gaagatctga actatgcccg tcgcctggca cgcgatgcag gatgtgatgc
    23281 gaaaattgtt gccaaggttg aacgtgcgga agccgtttgc agccaggatg caatggatga
    23341 catcatcctc gcctctgacg tggtaatggt tgcacgcggc gacctcggtg tggaaattgg
    23401 cgacccggaa ctggtcggca ttcagaaagc gttgatccgt cgtgcgcgtc agcttaaccg
    23461 cgcggtaatc acggcgaccc agatgatgga gtcaatgatt actaacccga tgccgacgcg
    23521 tgcagaagtc atggacgtag caaacgccgt tctggatggt actgacgctg tgatgctgtc
    23581 tgcagaaact gccgctgggc agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg
    23641 cctgggtgcg gaaaaaatcc cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt
    23701 cgacaatgtg gaagaagcta ttgccatgtc agcaatgtac gcagctaacc acctgaaagg
    23761 cgttacggcg attattacca tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat
    23821 cagctctggt ctaccaattt tcgccatgtc gcgccatgaa cgtacgctga acctgactgc
    23881 tctctatcgt ggcgttacgc cggtgcactt tgatagcgct aatgacggcg tagcagctgc
    23941 cagcgaagcg gttaatctgc tacgcgataa aggttacttg atgtctggtg acctggtgat
    24001 tgtcacccag ggcgacgtga tgagtaccgt gggttctact aataccacgc gtattttaac
    24061 ggtagagtaa tctcacctgc cccggccgct gatgaccggg gtatttagca acacataaat
    24121 taggactact cattcaatcc aatcataatc acagccccct gtaaaatgaa tacagggata
    24181 gcctgaatgg agatctaaat agcttgcaag acgtcgcata cccgcttcct ctgcaacact
    24241 atatccaata actgccatcg gtaagccact atctttggag tattgtgtct gcacccatgt
    24301 tttaattgaa tcgttaatcg taatataata atcagcctga tattccatat attgtattgg
    24361 atcacaaaaa cagcctgccc caatcccaat agttctaact attctggctc tatctccata
    24421 aaattggatc ccaggaagat tataaatttt caaatttttg gcaaaacgtt tggtaatatt
    24481 aatggcactg tcaggttcta tagcatatac atggtacata tcatcagaag caacaatatt
    24541 tgtaacatcc agttctaact gctgagccaa agccttagac attccgaacg taggctctcg
    24601 atccatgaca tcatggcaac gaatgattac caaattattt ttatgcaaaa actccttttt
    24661 ttgctcaatt atttttaaat aactgagttc accgcgcgta taatccatta tttttctggc
    24721 ccatgaaaac tc
//
LOCUS       DAZFWL020000064        24482 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFWL020000064 DAZFWL020000000
VERSION     DAZFWL020000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.064"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..363
                     /locus_tag="ACHKNP_RS13660"
                     /old_locus_tag="pgaptmp_002732"
     CDS             <1..363
                     /locus_tag="ACHKNP_RS13660"
                     /old_locus_tag="pgaptmp_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HGU4704871.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIAQQNSFKHT"
     gene            519..2054
                     /gene="gadC"
                     /locus_tag="ACHKNP_RS13665"
                     /old_locus_tag="pgaptmp_002733"
     CDS             519..2054
                     /gene="gadC"
                     /locus_tag="ACHKNP_RS13665"
                     /old_locus_tag="pgaptmp_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HGU4704872.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2185..3504
                     /locus_tag="ACHKNP_RS13670"
                     /old_locus_tag="pgaptmp_002734"
     CDS             2185..3504
                     /locus_tag="ACHKNP_RS13670"
                     /old_locus_tag="pgaptmp_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HGU4704873.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLPQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVQELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3881..5263
                     /gene="dosC"
                     /locus_tag="ACHKNP_RS13675"
                     /old_locus_tag="pgaptmp_002735"
     CDS             3881..5263
                     /gene="dosC"
                     /locus_tag="ACHKNP_RS13675"
                     /old_locus_tag="pgaptmp_002735"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HGU4704874.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5288..7687
                     /gene="dosP"
                     /locus_tag="ACHKNP_RS13680"
                     /old_locus_tag="pgaptmp_002736"
                     /gene_synonym="pdeO"
     CDS             5288..7687
                     /gene="dosP"
                     /locus_tag="ACHKNP_RS13680"
                     /old_locus_tag="pgaptmp_002736"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HGU4704875.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7945..8526
                     /gene="ddpX"
                     /locus_tag="ACHKNP_RS13685"
                     /old_locus_tag="pgaptmp_002737"
     CDS             7945..8526
                     /gene="ddpX"
                     /locus_tag="ACHKNP_RS13685"
                     /old_locus_tag="pgaptmp_002737"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HGU4704876.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8540..10090
                     /locus_tag="ACHKNP_RS13690"
                     /old_locus_tag="pgaptmp_002738"
     CDS             8540..10090
                     /locus_tag="ACHKNP_RS13690"
                     /old_locus_tag="pgaptmp_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4704877.1"
                     /translation="MKRSISFRPTLLALVLATNFPVSHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTNVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10092..11114
                     /locus_tag="ACHKNP_RS13695"
                     /old_locus_tag="pgaptmp_002739"
     CDS             10092..11114
                     /locus_tag="ACHKNP_RS13695"
                     /old_locus_tag="pgaptmp_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4704878.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11111..12007
                     /gene="ddpC"
                     /locus_tag="ACHKNP_RS13700"
                     /old_locus_tag="pgaptmp_002740"
     CDS             11111..12007
                     /gene="ddpC"
                     /locus_tag="ACHKNP_RS13700"
                     /old_locus_tag="pgaptmp_002740"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HGU4704879.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LALAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12004..12990
                     /locus_tag="ACHKNP_RS13705"
                     /old_locus_tag="pgaptmp_002741"
     CDS             12004..12990
                     /locus_tag="ACHKNP_RS13705"
                     /old_locus_tag="pgaptmp_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4704880.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12983..13909
                     /locus_tag="ACHKNP_RS13710"
                     /old_locus_tag="pgaptmp_002742"
     CDS             12983..13909
                     /locus_tag="ACHKNP_RS13710"
                     /old_locus_tag="pgaptmp_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HGU4704881.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTVPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(13965..14396)
                     /gene="osmC"
                     /locus_tag="ACHKNP_RS13715"
                     /old_locus_tag="pgaptmp_002743"
     CDS             complement(13965..14396)
                     /gene="osmC"
                     /locus_tag="ACHKNP_RS13715"
                     /old_locus_tag="pgaptmp_002743"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HGU4704882.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14742..14957
                     /gene="bdm"
                     /locus_tag="ACHKNP_RS13720"
                     /old_locus_tag="pgaptmp_002744"
     CDS             14742..14957
                     /gene="bdm"
                     /locus_tag="ACHKNP_RS13720"
                     /old_locus_tag="pgaptmp_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HGU4704883.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15059..15196
                     /gene="sra"
                     /locus_tag="ACHKNP_RS13725"
                     /old_locus_tag="pgaptmp_002745"
     CDS             15059..15196
                     /gene="sra"
                     /locus_tag="ACHKNP_RS13725"
                     /old_locus_tag="pgaptmp_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HGU4704884.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15353..17050
                     /gene="maeA"
                     /locus_tag="ACHKNP_RS13730"
                     /old_locus_tag="pgaptmp_002746"
     CDS             15353..17050
                     /gene="maeA"
                     /locus_tag="ACHKNP_RS13730"
                     /old_locus_tag="pgaptmp_002746"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU4704885.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17184..18194
                     /gene="adhP"
                     /locus_tag="ACHKNP_RS13735"
                     /old_locus_tag="pgaptmp_002747"
     CDS             17184..18194
                     /gene="adhP"
                     /locus_tag="ACHKNP_RS13735"
                     /old_locus_tag="pgaptmp_002747"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGU4704886.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            18341..18625
                     /locus_tag="ACHKNP_RS13740"
                     /old_locus_tag="pgaptmp_002748"
     CDS             18341..18625
                     /locus_tag="ACHKNP_RS13740"
                     /old_locus_tag="pgaptmp_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4704887.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(18676..19329)
                     /gene="fdnI"
                     /locus_tag="ACHKNP_RS13745"
                     /old_locus_tag="pgaptmp_002749"
     CDS             complement(18676..19329)
                     /gene="fdnI"
                     /locus_tag="ACHKNP_RS13745"
                     /old_locus_tag="pgaptmp_002749"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HGU4704888.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19322..20206)
                     /gene="fdnH"
                     /locus_tag="ACHKNP_RS13750"
                     /old_locus_tag="pgaptmp_002750"
     CDS             complement(19322..20206)
                     /gene="fdnH"
                     /locus_tag="ACHKNP_RS13750"
                     /old_locus_tag="pgaptmp_002750"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HGU4704889.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20219..23266)
                     /gene="fdnG"
                     /locus_tag="ACHKNP_RS13755"
                     /old_locus_tag="pgaptmp_002751"
     CDS             complement(20219..23266)
                     /gene="fdnG"
                     /locus_tag="ACHKNP_RS13755"
                     /old_locus_tag="pgaptmp_002751"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22679..22681),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU4704890.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23498..24379
                     /gene="yddG"
                     /locus_tag="ACHKNP_RS13760"
                     /old_locus_tag="pgaptmp_002752"
     CDS             23498..24379
                     /gene="yddG"
                     /locus_tag="ACHKNP_RS13760"
                     /old_locus_tag="pgaptmp_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HGU4704891.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5992 a         6210 c         6190 g         6090 t
ORIGIN      
        1 taccaggttg ccgcttatct ggcggatgaa atcgccaaac tggggccgta tgagttcatc
       61 tgtacgggtc gcccggacga aggcatcccg gcggtttgct tcaaactgaa agatggtgaa
      121 gatccgggat acaccctgta cgacctctct gaacgtctgc gtctgcgcgg ctggcaggtt
      181 ccggccttca ctctcggcgg tgaagccacc gacatcgtgg tgatgcgcat tatgtgtcgt
      241 cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc ctccctgaaa
      301 tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt taaacatacc
      361 tgataacgtt taacggtaac ggtgtcccga aacgaacccg tttcgggaca atttccaaag
      421 tctgttcact ggcattagca acggaaaata ttgttctgaa tacgcttcag aacaaaacag
      481 gtgcggttcc gacaggaata ccgttttagg gggataatat ggctacatca gtacagacag
      541 gtaaagctaa gcagctcaca ttactcggat tctttgccat aacggcatcg atggtaatgg
      601 ctgtttatga ataccctacc ttcgcaacat cgggcttttc attagtcttc ttcctgctat
      661 taggcgggat tttatggttt attcccgtgg gactttgtgc tgcggaaatg gccaccgtcg
      721 acggctggga agaaggtggt gtcttcgcct gggtatcaaa tactctgggg ccgagatggg
      781 gatttgcagc gatctcattt ggctatctgc aaatcgccat tggttttatt ccgatgctct
      841 atttcgtgtt aggggcactc tcctacatcc tgaaatggcc agcgctaaat gaagacccca
      901 ttaccaaaac tattgcagca ctcatcattc tttgggcgct ggcattaacg cagtttggtg
      961 gcacgaaata cacggcgcga attgctaaag ttggcttctt cgccggtatc ctgttacctg
     1021 catttatttt gatcgcatta gcggctattt atctgcactc cggtgccccc gttgctatcg
     1081 aaatggattc gaagaccttc ttccctgact tctctaaagt gggcaccctg gttgtatttg
     1141 ttgccttcat tttgagttat atgggcgtag aagcttccgc aacccacgtc aatgaaatga
     1201 gtaacccagg gcgcgactat ccactggcta tgttactgct gatggtggcg gcaatctgct
     1261 taagctctgt tggtggtttg tctattgcga tggtcattcc gggtaatgaa atcaacctct
     1321 ccgcaggggt aatgcaaacc tttaccgttc tgatgtccca tgtggcaccg gaaattgagt
     1381 ggacggttcg cgtgatctcc gcactgctgt tgctgggtgt tctggcggaa atcgcctcct
     1441 ggattgttgg tccttctcgc gggatgtatg tcacagcgca gaaaaacctg ctgccagcgg
     1501 cattcgctaa aatgaacaaa aatggcgtac cggtaacgct ggtcatttcg cagctggtga
     1561 ttacttctat cgcgttgatc atcctcacca ataccggtgg cggtaacaac atgtccttcc
     1621 tgatcgctct ggcgctgacg gtggtgattt atctgtgtgc ttatttcatg ctgtttattg
     1681 gctacattgt gttggttctt aaacatcctg acttaaaacg cacatttaat atccctggtg
     1741 gtaaaggggt gaaactggtc gtggcaattg tcggtctgct gacttcaatt atggcgttta
     1801 ttgtttcctt cctgccgccg gataacatcc agggtgattc taccgatatg tatgttgaat
     1861 tactggttgt tagtttcctg gtggtacttg ccctgccctt tattctctat gctgttcatg
     1921 atcgtaaagg caaagcgaat accggcgtca ctctggagcc aatcaacagt cagaacgcac
     1981 caaaaggtca cttcttcctg cacccgcgtg cacgttcacc acactatatt gtgatgaatg
     2041 acaagaaaca ctaaccatgt ctgaatggtt ataagacaag ggagcgataa gtcatcgctc
     2101 cctttttcgt gcttgctgtc gtattgactt aaccgggata agtacgagaa tgagcgcaca
     2161 tctgtttacc ggaaaccagc acatatggat atctgctccc gaaacaagaa attaacgatt
     2221 agaagaccag cgatactagt tgcactggca cttttactgt gtagttgtaa aagcacgcct
     2281 ccagagtcca tggtgacacc accagcaggt tcaaagccac cagccacgac gcaacaatcg
     2341 tcacaaccga tgcgtggcat ctggctggcc acggtttctc ggctcgactg gccaccggtt
     2401 tcctcggtta acattagtaa ccccaccagc cgggcccgtg tacaacaaca ggcgatgatc
     2461 gacaaactgg atcatctgca acgtctcggc ataaacacgg tctttttcca ggtcaagccg
     2521 gacggtaccg ccctgtggcc atcgaaaatt ttgccgtggt ccgatcttat gaccggtaag
     2581 attggtgaaa atccgggtta cgatccgctg caatttatgc tcgacgaagc ccacaagcgt
     2641 gggatgaaag tacacgcctg gtttaacccc tatcgcgtat cggttaatac gaagcccggt
     2701 actatcaggg aactgaatag cactctgcct caacaaccgg cgagcgtcta tgtgcaacac
     2761 cgcgactgga tcagaacgtc tggcgatcgc tttgtcctcg acccgggcat ccctgaggtt
     2821 caggactgga tcacatcaat agtcgcagaa gtggtttccc gctatcccgt agatggcgtg
     2881 cagtttgacg actatttcta tacggagtca ccgggttcac ggctaaatga taacgaaacg
     2941 taccgtaaat acggaggcgc atttgcgtca aaagcagact ggcggcgcaa caatactcag
     3001 cagttaattg caaaggtatc gcacaccatt aaaagcatta agccgggagt cgaatttggt
     3061 gttagcccgg caggcgtgtg gcgtaaccga tcacacgatc cgctcggttc cgatacccga
     3121 ggcgcggcag cctatgacga atcctacgct gacacccgtc gatgggtgga acaaggattg
     3181 ctggattaca ttgctcccca aatttactgg ccgttctcac ggagtgccgc gcgttatgac
     3241 gtgttggcaa aatggtgggc ggatgtcgtt aaaccgacca ggacccgcct gtatatcggt
     3301 atcgccttct ataaagtggg tgaaccttca aagatagagc cagactggat gattaacggc
     3361 ggcgtacagg aactgaaaaa gcagctcgat cttaacgatg cggtaccaga aattagcggc
     3421 accatcttgt tccgtgagga ctatctgaat aaaccacaga ctcaacaagc ggtcagctat
     3481 ctgcaaagtc gttggggcag ttaaactccc attcttactt tgtaacaagc cggtgcttta
     3541 cccaccggct tgttaccctc ttgaactatc ccactactaa tcatgcttac taaagtcaaa
     3601 ttaaccacgc aattaaccac acttagttaa acagccataa atcccctaat aaaaatgtat
     3661 gaatatgaaa aaacatacga tattcgctgt caccgaaaaa taattacgat gggctatact
     3721 cttctgaaga acagtagcgt agattaaaac tgtcctaagt cgcttatttc ttttcagtat
     3781 atcttcatat ttcaggagaa tatggtgcag ttagtcgatc tcgcacgttg cgtttcgttt
     3841 tgattccgtc tgatttccca gccttataag ggtgtgaatt atggagatgt attttaaaag
     3901 aatgaaagat gagtggaccg gacttgtcga acaggcagat cctctcattc gtgctaaagc
     3961 cgcagaaatt gccgttgcgc atgctcatta tctgagtatc gagttttatc gaattgtccg
     4021 catcgacccg catgccgaag aattcttgag taatgaacaa gttgagcggc agttgaaaag
     4081 tgcgatggaa cgctggatta ttaacgtgct ttctgcccag gttgacgatg tcgaaaggct
     4141 aatacaaatc cagcataccg tcgcggaagt gcatgcccgc ataggaattc cggtagaaat
     4201 tgtcgagatg gggtttcggg tgctgaaaaa aatcctctat ccggtaatct tctcttcgga
     4261 ttattctgcc gcagaaaaac ttcaggtcta ccatttctcg attaacagta ttgatatcgc
     4321 tatggaagtg atgacccgcg cgtttacctt tagtgacagt agtgcctcaa aggaagatga
     4381 aaactatcgt atcttctcgt tactagaaaa cgccgaagaa gaaaaagaac ggcaaatagc
     4441 ctcaatactt tcatgggaaa tagatattat ctataaaatc ctgctggatt ctgatttagg
     4501 cagtagtttg cctttaagcc aggctgattt tggcctgtgg tttaaccata aaggtcgaca
     4561 ttattttagt ggcattgccg aagtaggcca tatctcccgt ctgattcagg atttcgacgg
     4621 tatattcaat caaaccatgc gtaacaccag gaatttgaat aacagaagtc tgcgggtgaa
     4681 atttttatta cagataagaa atactgtatc gcaaattatt accttgctgc gtgaattgtt
     4741 tgaagaagta tcgcgccacg aagtcggtat ggatgtactg acgaaattac ttaaccgccg
     4801 tttcctgccg actatcttca aacgcgaaat tgcccatgcc aaccggaccg gtacaccact
     4861 gtcagtgctg attattgacg ttgataaatt caaagagatc aacgatacgt ggggccataa
     4921 cactggcgat gaaattctgc gtaaagtctc tcaggccttt tatgacaacg tccgcagtag
     4981 tgattatgtt tttcgctacg ggggcgatga atttatcatt gttttgactg aagcttctga
     5041 aaacgaaacg ttacgtaccg cagaacgtat tcgcagtcgg gtggagaaaa ccaaactgaa
     5101 agccgcaaac ggcgaggata ttgccctctc actttccatc ggtgccgcca tgtttaatgg
     5161 tcatcctgac tatgagcgcc tcattcaaat agccgatgaa gctctgtata tcgccaaaag
     5221 acgaggtaga aaccgtgttg aactctggaa agccagtctt tagatgcgcc aggatgcaga
     5281 ggtaatcatg aagctaaccg atgcggataa tgccgccgat ggcatttttt tccccgccct
     5341 tgagcaaaat atgatgggtg cggtgttaat taacgaaaat gatgaagtga tgtttttcaa
     5401 ccccgccgca gagaagctct gggggtacaa acgtgaagaa gtcattggca ataacattga
     5461 tatgctgatt ccgcgggatt tgcgtcctgc gcatcctgaa tacattcgtc ataaccgtga
     5521 aggcggtaaa gcgcgtgtcg aggggatgag tcgggagctg cagctggaga aaaaagacgg
     5581 cagtaaaatc tggacccgtt ttgcgctatc gaaagtgagc gccgagggga aagtttatta
     5641 cctggcgctg gtacgggatg ccagcgtaga aatggcgcaa aaagaacaga ctcgacaatt
     5701 gattattgcc gttgaccatc tcgaccgacc ggtgattgtc ctcgatccgg aacgccatat
     5761 tgtgcagtgc aatcgcgcat ttaccgaaat gtttggttac tgcattagcg aagccagcgg
     5821 tatgcagccc gatacactcc tgaacattcc tgaattccct gccgataacc gcattcgttt
     5881 acaacagttg ctatggaaaa ccgcccgcga tcaggacgaa tttctgctgt tgacgcgcac
     5941 cggtgaaaaa atctggatta aagcctctat cagcccggtt tatgacgtgc tcgcgcatct
     6001 gcagaacctg gtaatgactt tctcggatat caccgaagaa cggcaaatcc gccagcttga
     6061 aggcaatatt ctcgccgcca tgtgcagcag cccgccattt catgaaatgg gggaaatcat
     6121 ttgtcgtaac atcgaatctg ttctcaacga atcgcatgtt tcgctgttcg cactgcgcaa
     6181 cgggatgccg atacactggg cgtcatcttc gcacggtgca gaagttcaaa atgcgcaaag
     6241 ctggtcagcg accattcgtc agcgtgatgg cgcgcctgcg gggatcctgc aaattaaaac
     6301 ctcgtcagga gcagaaacca gcgcctttat cgaacgcgtg gcagatatca gtcagcatat
     6361 ggccgcgctg gcgctggaac aggaaaaaag ccgccagcat attgaacaac tcatccaatt
     6421 tgatccgatg accggtctgc caaatcgcaa taacctgcac aattacctcg atgacctggt
     6481 cgacaaagcc gtctctccgg tggtgtatct catcggtgtt gaccatattc aggatgtgat
     6541 tgatagcctt ggctatgcgt gggccgatca ggcattgctg gaagtggtca atcgctttcg
     6601 tgaaaaactc aaaccggatc agtatctctg tcgtatcgaa ggtacgcagt ttgtcctcgt
     6661 gagcctggaa aacgacgtca gtaacattac ccagatcgcc gatgagctac ggaatgtggt
     6721 cagcaagccg ataatgattg acgataaacc attcccgctt accttgagta ttggcatcag
     6781 ctacgacgtg ggtaaaaacc gcgattacct actctccact gctcacaatg caatggatta
     6841 tattcgcaag aatggcggta acggctggca gttcttcagc ccggcgatga acgaaatggt
     6901 aaaagagcgt ttggttttag gcgcagcgct gaaagaagcg attagcaata accaactgaa
     6961 actggtttac cagccgcaaa tcttcgcaga aacgggtgaa ctgtacggca tcgaagccct
     7021 tgctcgctgg cacgatcccc agcatggtca tgtaccccct tcacggttta ttcctctcgc
     7081 agaagagatt ggtgaaatcg aaaatattgg gcgctgggtc atcgcggaag cttgccgtca
     7141 gttagcagaa tggcgtagcc agaatattca tatcccggcg ttatccgtga acttgtcggc
     7201 gctgcacttt cgcagtaatc agctgcctaa tcaggtgtct gatgcaatgc aagcctgggg
     7261 tattgacggc caccagctga cggtagaaat cacggaaagc atgatgatgg aacacgatac
     7321 cgaaatcttt aagcgcattc agatcctgcg tgatatgggc gtgggcttat cggtagatga
     7381 ttttggtacg ggcttttccg gattatcccg cttagtcagt cttccggtaa cggaaatcaa
     7441 aattgacaaa agttttgtcg atcgttgtct gaccgaaaaa cgcatccttg ccttacttga
     7501 agccattacc agcattgggc aaagcctcaa tttaaccgtc gtggcggaag gcgtcgaaac
     7561 caaagagcaa tttgagatgc tacgcaagat ccactgtcgc gttattcagg gatatttctt
     7621 ttcccgcccc ctacccgccg aagaaattcc aggctggatg agcagcgtgt taccgctgaa
     7681 aatctgacaa attcctctcg cccgcactcg cgggttttca tttaacgtga cactgtcact
     7741 taaatactgt gatttcagct acgattccgg gagattcctt ccttaacttc ccctcttgca
     7801 ccaaaactgc actacgctgc acaaatgttg atcaaaagtt aaaaaatata actttaagcc
     7861 agcattctgg cgcttatccc ggcatggcat gagatctgca taagcggaaa gcgcagcaat
     7921 ttttgtctta tacaggaacc gtttatgtcg gataccaccg aactggttga tttagccgta
     7981 atcttccctg atctggagat cgaattgaaa tacgcctgcg ctgataacat cacaggtaaa
     8041 gctatttatc agcaagcgcg ttgtctgcta cacaaggatg cgattaccgc gctggcgaaa
     8101 agtatcagta tcgcccagct gtcaggctta caactggtga tttacgatgc gtatcgccca
     8161 caacaagcac aggcgatgtt gtggcaagcc tgcccagacc cgcaatatgt tgttgatgtg
     8221 acggtcggtt ctaatcacag ccgtggcacg gcgatcgacc tgacgcttcg tgatgagcac
     8281 gggaacatcc tcgatatggg cgcagggttc gatgaaatgc acgagcgctc ccatgcctat
     8341 cacccttccg tcccgcccgc cgctcagcgc aatcggctgt tgctgaatgc gataatgact
     8401 ggtggcggtt ttgtcggtat ctccagcgaa tggtggcact tcgaattacc ccaggcagcg
     8461 agttaccctc tgctcgctga tcaattctct tgttttatat cccctggcac acagcacgtc
     8521 agttaatcag gaaactgtca tgaagagatc gatatcgttt cgtcccacat tgctcgcgct
     8581 cgtccttgcc acaaatttcc cggtttcgca cgccgccgta ccgaaagata tgctggtgat
     8641 tggtaaggcc gccgatccac aaaccctcga cccggcggta acaatagata ataacgactg
     8701 gacggtgacc tacccgtctt atcagcggct ggttcagtac aaaacggacg gtgataaagg
     8761 ctcaaccaac gttgaaggcg atctggcaag tagctggaaa gcgtctgacg atcaaaaaga
     8821 gtggacgttc accctgaaag ataatgctaa atttgccgat ggcacacctg tcactgccga
     8881 agcagtaaaa ctttcttttg agcggctact aaaaatcggc caggggccag cagaagcatt
     8941 tcccaaagat ttaaagattg atgctcccga cgaacatacg gtgaagttta cccttagcca
     9001 accattcgca ccgttcctct acacgctggc gaatgacggt gcatccatta tcaatccggc
     9061 ggtcttaaag gaacatgcag cggatgatgc tcgcggcttc ctcgcgcaaa ataccgccgg
     9121 ttccggacca tttatgctga aaagctggca aaaaggtcag caattagttc tggtgccaaa
     9181 tccgcattac cccggcaata aaccgaactt taagcgagta tcggtaaaaa ttatcggtga
     9241 aagtgcctcc cgtcgcctgc agctctcccg tggcgacatt gacattgccg atgcgctgcc
     9301 ggtggatcaa ctcaacgccc tgaagcagga aaataaagtc aatgtggcag agtatccgtc
     9361 actgcgcgtt acctatctgt atctgaataa cagcaaagcg cctcttaatc aggcggatct
     9421 acgtcgggcc atttcctggt ctaccgatta tcagggcatg gttaacggca ttctgagtgg
     9481 taacggaaaa cagatgcgcg gcccgattcc ggaaggcatg tggggctacg atgcgacggc
     9541 aatgcaatac aaccatgacg aaacgaaagc caaagccgaa tgggataaag tgacgagcaa
     9601 acccaccagc ctgacgtttc tctactccga taacgatccg aactgggagc ctattgcttt
     9661 ggcgacacaa tccagtctca acaagctggg tatcaatgtg aagctggaaa agctggcgaa
     9721 cgccaccatg cgcgacagag tgggtaaagg tgattacgac attgcgattg gcaactggag
     9781 tccggatttt gccgacccgt atatgtttat gaattactgg tttgagtcag acaaaaaagg
     9841 tctgccgggt aaccgctcgt tctatgaaaa cagtgaggtc gataagttac tgcgcaatgc
     9901 gcttgcgacc accgaccaga cgcagcgtac ccgggactac cagcaggcac agaaaatcgt
     9961 cattgatgac gctgcttatg tgtacctgtt ccagaaaaac taccaactgg cgatgaacaa
    10021 agaggtgaaa ggctttgtgt tcaatcccat gctggaacag gtcttcaata tcaataccat
    10081 gagtaaataa cgtgaccttc tggagtattt tacgccaacg ctgctggggg ctggtgctcg
    10141 tggtggcggg cgtctgcgtg attacgttta ttatctcgca tctgatccct ggcgatccgg
    10201 cgcggttact ggcgggtgac cgcgccagcg atgctatcgt ggaaaatatt cgccagcaac
    10261 tgggactgga ccagccactg tatgtacagt tttaccgcta cgtcagcgac ctgtttcatg
    10321 gtgacctggg aacatccatt cgtaccgggc gtccggtgct ggaagagttg cgtatatttt
    10381 tcccggcgac gctggagctg gcttttggtg ccctgctgct ggcactcctg attggcatcc
    10441 cgctgggcat actctctgca gtctggcgaa atcgctggct ggatcatctg gtgcgaataa
    10501 tggccattac cggaatctcc acacctgcgt tctggcttgg gctgggcgtc attgtgctgt
    10561 tttatggtca tctgcaaatt cttcccggcg gcggaaggct tgatgactgg ctggatccac
    10621 caacgcacgt taccggcttt tatctgctcg atgcgctgct tgaaggcaac ggtgaagtct
    10681 tcttcaatgc gttgcaacat ctcatcttac cggcattaac gctggcgttc gttcacctgg
    10741 gaattgtcgc tcgccagatc cgctcagcga tgctggaaca attgagtgaa gactacattc
    10801 gtaccgcccg ggccagcggc ttgcccggct ggtatatcgt tttatgttat gcgctaccca
    10861 atgcgttgat cccatcgatt accgtattgg gtttggcgct gggcgatttg ttgtatggcg
    10921 cagtgctcac cgaaaccgtt tttgcctggc ccggaatggg tgcatgggta gtaacatcaa
    10981 tacaggcgct cgacttcccg gcagtgatgg gctttgccgt cgtggtttca tttgcttatg
    11041 tgctggtcaa cctggtggtg gatttgctct atttgtggat tgatccgcgt atcggacgtg
    11101 gaggtggtga atgatgctaa gcgaggaaac gtccgccgta cgcccacaaa aacaaacgcg
    11161 atttaacggt gcaaaactgg tatggatgct gaaaggcagt ccgctcaccg tgaccggcgc
    11221 agtcatcatt gtattaatgc tattgatgat gattttttca ccgtggctgg cgacgcatga
    11281 tcccaacgcc attgatttaa ccgcccgcct tttgccgcct tctgcggcgc actggtttgg
    11341 caccgatgaa gtgggacgcg atctgttcag ccgcgtactg gtcggcagtc agcaatcaat
    11401 tctcgccgga ttagtcgtgg ttgccattgc gggtatgatt ggttcgctac tcggatgtct
    11461 atccggtgtg cttggcggac gcgcagacgc catcattatg cgcatcatgg acattatgct
    11521 gtcgattcct tcgttggtac tgacaatggc actggctgcc gctctcgggc cgagtttatt
    11581 taacgccatg ctggcgcttg ctattgtgcg aattcccttt tatgtgcgcc tggcgcgggg
    11641 gcaagcatta gttgtacgcc agtataccta tgttcaggcg gcgaaaacct ttggtgcgtc
    11701 gcgttggcat ctgatcagct ggcatatttt acgtaactcc ctgccgccgc tgatcgtgca
    11761 ggcatcgctg gatatcggta gcgcgatttt aatggccgcc acgttgggat ttattggcct
    11821 gggtgctcaa caaccgagtg ctgaatgggg cgcgatggta gcgaatggtc gcaactatgt
    11881 actcgatcag tggtggtatt gcgcatttcc gggggcagcg attttgctta ccgccgttgg
    11941 gtttaatctc tttggtgatg gtattcgcga tctgcttgac ccgaaagcag gaggaaagca
    12001 gtcatgaccc aacccgttct ggacattcaa cagctgcatt tgagtttccc cggttttaac
    12061 ggtgatgttc acgcgctcaa caatgtgtcc ttgcagatta accgcggcga aattgtcggt
    12121 ctggtgggag aatccggctc aggtaaatca gtcaccgcaa tgctgattat gcgtctgcta
    12181 cagacgggca gttattgcgt acatcggggg cagatttcac tgctgggaga cgatgttctt
    12241 aacgctcggg aaaaacagct tcgtcagtgg cgcggcgcac gagtggcgat gatctttcag
    12301 gaaccgatga ccgccctcaa tccgacactt cgaataggtc ttcagatgat ggacgtgatc
    12361 cgccatcatc aaccaataag tcgtcgggaa gccagagcta aagcgattgc cctgctggaa
    12421 gagatgcaaa tcccggatgc cgtggaagtt atgtcgcgct atccgtttga gctttcaggt
    12481 ggtatgcgcc agcgggtaat gattgcgctg gcattctcct gcgagccgca attgattatt
    12541 gccgacgaac cgactacggc gctggacgtc acggtgcagt tgcaggtact gcgtttgctt
    12601 aaacataaag cccgcgccag tggaactgcg gttctgttca tcagccatga tatggccgtg
    12661 gtgtcgcaac tgtgcgatag cgtttacgtg atgtatgccg gaagcgtaat tgaaagcggc
    12721 gtgacggcag atgttatcca tcatccccgg catccgtata ccattggttt gctccaatgc
    12781 gcaccagaac atggagtacc acgccagcta ttacccgcca ttccagggac ggtaccaaac
    12841 ctcacccatt tgcctgaagg ctgcgctttt cgcgatcgtt gctatgcggc aggtgcacag
    12901 tgtgaaaacg tcccggcgct gacagcgtgt ggtgacaaca accagcgctg cgcctgttgg
    12961 tatccgcagc aggaggtcat aagtgtctga cacgttatta acgttacgcg acgtccatat
    13021 caatttcccg gcccgtaaaa actggcttgg taaaactacg gaacatgttc atgccattaa
    13081 tggtattgat ttacagatcc gccgtggtga aaccttaggg atcgtcggcg agtcaggctg
    13141 cggcaaaagc accctcgcac agcttttaat gggcatgctg caaccgagcc acgggcagta
    13201 catccgttct ggctcacaac gcattatgca gatggtgttt caggacccgc tctcttcgct
    13261 caatccgcgc ttaccggtgt ggcgcatcat cacagaaccg ctctggatag ctaagcgtag
    13321 tagtgaacaa cagcggcgag cgttggcaga ggagctggct gtgcaggtgg gtattcgtcc
    13381 ggagtatctc gaccgcctgc ctcatgcgtt ctccggcggg cagcggcaac gcatcgccat
    13441 tgccagagca ctctcttcgc agcctgacgt gattgtgctt gatgagccaa cctcggcgct
    13501 ggatatctcc gtgcaggcgc agatcctcaa tttactggta acgctacagg aaaatcacgg
    13561 gctgacttat gtgctgattt cacacaatgt ctcggtgata cgtcatatga gcgatcgggt
    13621 ggcggtgatg tatctcgggc agattgtgga actaggggaa gcgcaacagg tgttgactgt
    13681 tccggcacat ccatataccc gattattgct ggattccctc cccgccattg ataaaccgct
    13741 ggaggaagaa tgggcattac gtaaaacgga tctgccggga aaccgcacgt tgccgcaagg
    13801 ctgttttttc tacgaacgtt gccctctggc aacccacgga tgtgaagtcc ggcaatcatt
    13861 aacaataagg gaggacggac gtgagatccg gtgctggcgg gcgctgtaga gatgctaaca
    13921 acgcatcagg cattcacgcg cctgacgcgt catccggcaa tgctttacga tttcaactgg
    13981 taatcaagcg taatttccgc tttcagtacc tgagaaaccg ggcatcctgc ttttgctttc
    14041 tggatgatgc cgtcaaaggt agaggcatca atacccggca ccgcaacttc actcttcagt
    14101 gcgattttcg taatcgcaaa accggcatcc actttatcca gcgacacatc ggcggtggta
    14161 tcaatcgatg ttggcgtaaa tcccgcttcc cccagcatta atgaaagcgc cattgagaaa
    14221 catgcggcat gcgctgcgcc aatcagttct tcagggttag ttcctttttc gccttcaaaa
    14281 cgcgtgttaa atccatacgg ctgttggttc agcacaccac tctcggtgga tactgttccc
    14341 ttcccgcgtt tgatatcgcc ttcccagtgt gcctgacctt tcttatggat tgtcattgtt
    14401 gctctcctgt gggcttaaaa aggaaattat agactacgtg agaaacagca cgaggataag
    14461 caggatattc cgaataaaaa tccggcagaa atattacttc tgcacttaaa ttaaatttcc
    14521 acaatagata accgggaata tataacccaa gagatattgc attaatatta atcaactcac
    14581 cacaataata atacctatca atatcatgag gttaaaatat aaaaatcatc aaccaggact
    14641 aatcttaaca acgaagcggc aaatatttgc catgctgaat gtgctttata aatctgcgat
    14701 ccgtagcaga caccataaat acacagacac ggagaatcac tatgtttact tattatcagg
    14761 cagaaaattc aacagcagaa cccgctctgg ttaatgccat tgagcaaggc cttcgggcag
    14821 agcacggtgt tgtcactgaa gatgacattc tcatggagct gaccaaatgg gtggaagctt
    14881 ctgataacga catcctcagt gatatctacc agcaaacgat caattatgtg gtcagtggcc
    14941 agcaccctac gctttaaggt gctatgcttg atcggcaacc taatttaggg gtttagcacg
    15001 tgtttcttcg ctacggcgat gttgtcctta aaactagcaa caggattgag gagttaaaat
    15061 gaaatcgaac cgtcaggcac gtcatattct tggactggac cataaaattt ctaaccagcg
    15121 caaaatagtt accgaaggtg acaaatccag cgtggtaaat aatccaaccg gcagaaaacg
    15181 ccccgctgaa aagtaattca taaccatcag tcctcaatga cgattaaaca ccattgcctg
    15241 cgcaatggtg tttttgtttt tatctgctct atacttgggg ccgacgccct ggcggtaaag
    15301 caaagacgat aaaagcgtgc cagggatgga tattcaaaaa agagtgagtg acatggaacc
    15361 aaaaacaaaa aaacagcgtt cgctttatat cccttacgct ggccctgtac tgctggaatt
    15421 tccgttgttg aataaaggca gtgccttcag catggaagaa cgccgtaact tcaacctgct
    15481 ggggttactg ccggaagtgg tcgaaaccat cgaagaacaa gcggaacgag catggatcca
    15541 gtatcaggga ttcaaaaccg aaatcgacaa acacatctac ctgcgtaaca tccaggacac
    15601 caacgaaacc ctcttctacc gtctggtaaa caatcatctt gatgagatga tgcctgttat
    15661 ttatacccca accgtcggcg cagcctgtga gcgtttttct gagatctacc gccgttcacg
    15721 cggcgtgttt atctcttacc agaaccggca caatatggac gatattctgc aaaacgtgcc
    15781 gaaccataat attaaagtga ttgtggtgac tgacggtgaa cgtattctgg ggcttggtga
    15841 ccagggcatc ggcgggatgg gcattccgat cggtaaactg tcgctctata ccgcctgtgg
    15901 cggcatcagc ccggcgtata cccttccggt ggtgctggat gtcggaacga acaaccaaca
    15961 gctgcttaac gatccgctgt atatgggctg gcgtaatcca cgtatcactg acgatgaata
    16021 ctatgaattc gttgatgaat ttatccaggc tgtgaaacaa cgctggccgg acgtgctgtt
    16081 gcagtttgaa gactttgctc aaaaaaatgc gatgccgtta cttaaccgct atcgcaatga
    16141 aatttgttct tttaacgatg acattcaggg cactgcggca gtaacagtcg gcacactgat
    16201 cgcagccagc cgcgcggcag gtggtcagtt aagcgagaaa aaaatcgtct tccttggcgc
    16261 aggttcagcg ggatgcggca ttgccgaaat gatcatcgcc cagacccagc gcgaaggatt
    16321 aagcgaggaa gcagcgcggc agaaagtctt tatggtcgat cgctttggcc tgctgacgga
    16381 caagatgccg aacctgctac ctttccagac caaactggtg cagaagcgcg aaaacctcag
    16441 tgactgggat accgacagcg atgtgttgtc actgctggat gtggtgcgca atgtaaaacc
    16501 agatattctg attggcgtct caggacagac cgggctgttt acggaagaga tcattcgtga
    16561 gatgcataaa cactgtccgc gtccgatcgt gatgccgctg tctaacccga cgtcacgcgt
    16621 ggaagccaca ccgcaggaca ttatcgcctg gaccgaaggt aacgcgctgg tcgccactgg
    16681 cagcccgttt aatccagtgg tatggaaaga taaaatctac cctatcgccc agtgtaacaa
    16741 cgcctttatt ttcccaggca tcgggctggg tgttattgct tccggcgcgt cacgtatcac
    16801 cgatgagatg ctgatgtcag caagtgaaac gcttgctcag tattcgccgc tggtgctgaa
    16861 cggcgaaggt ctggtactac cggaactgaa agatattcag aaagtctccc gcgcaattgc
    16921 gtttgcggtt ggcaaaatgg cgcagcagca aggcgtggcg gtgaaaacgt ctgccgaagc
    16981 tctgcaacaa gccattgacg ataatttctg gcaagccgaa taccgcgact accgccgtac
    17041 ctccatctaa gcctgcgccc ggtagtgaag gctaccgggc tatttccctc tccctttttc
    17101 agatctcatc catactgggt agtggcgaat aaatctcatt tgcctcacct gctatgcaga
    17161 acatcatccg aaaaggagga actatgaagg ctgcagttgt tacgaaggat catcatgttg
    17221 acgttacgga taaaacactg cgctcactga aacatggcga agccctgctg aaaatggagt
    17281 gttgtggtgt atgtcatacc gatcttcatg ttaagaatgg cgattttggt gacaaaactg
    17341 gggtaattct gggccatgaa ggcatcggtg tggtggcaga agtgggtcca ggtgtcacct
    17401 cattaaaacc aggcgatcgt gccagcgtgg cgtggttcta cgaaggatgc ggtcattgcg
    17461 aatactgtaa cagtggtaac gaaacgctct gccgttcagt taaaaatgcc ggatacagcg
    17521 ttgatggcgg gatggcggaa gagtgcatcg tggtcgccga ttacgcggta aaagtgccag
    17581 atggtctgga ctcggcggcg gccagcagca ttacctgtgc gggggtcacc acctacaaag
    17641 ccgttaaact gtcaaaaatt cgtcccggtc agtggattgc tatctacggt cttggcggtc
    17701 tgggtaacct cgccctgcaa tacgcgaaga atgtctttaa cgccaaagtg atcgccattg
    17761 atgtcaatga tgagcagtta aaactggcaa ccgaaatggg tgcagattta gcgattaact
    17821 cacgcaccga agacgccgcc aaaattgtgc aggagaaaac cggtggcgct cacgctgcgg
    17881 tggtaacagc agttgctaaa gctgcgttta actcggcagt tgatgctgtc cgtgcaggcg
    17941 gtcgtgttgt ggctgtcggt ctgccgccgg agtctatgag cctggatatc ccacgccttg
    18001 tgctggatgg cattgaagtg gtcggttcgc tggtcggcac gcgccaggat ctaaccgaag
    18061 ccttccagtt tgccgccgaa ggcaaagtgg tgccgaaagt cgccctgcgt ccgttagcgg
    18121 acatcaacac catctttacc gagatggaag aaggcaaaat ccgtggccgt atggtgattg
    18181 atttccgccg ttaagaggcc tttgctgcga gtgccagtcc tggtgtcgca gcatcgcact
    18241 ctctccgaca ttaaatggct aaatggatta ttgttccttc ctacgcaaca ataatgatag
    18301 tgttcctgat gaaggaacac tactgagagt aaagacactg atgaaaatgg ccaatcatcc
    18361 ccgcccgggg gacattattc aggaatcact ggacgaactt aatgtcagcc tgcgcgagtt
    18421 tgccagagca atggaaattg ctccctcaac ggcaagccga ttgctgaccg gaaaagcagc
    18481 tttgacacca gaaatggcga ttaaactttc cgtggtgatc ggcagttcgc cgcaaatgtg
    18541 gctgaatctg caaaacgcct ggagtctggc agaggcagaa aaaacggtgg atgtgtcgcg
    18601 actacgccgt ctggttacgc aataaaaaaa tggcgcttct taaagcgcca ttttcagctt
    18661 aaagttcaaa agagattata tcccttcttc actctctttt ttcgcttctg ccttctcgat
    18721 ttcacgatac cagcgcggat ggtgtttctt cgcccagcga cggctcacct tcccttcgat
    18781 catcccttta atcgaccctt tcacccagaa tgccatatac atatggatca ggatggcgtg
    18841 gatcaggatg atacccgcag ccgcgtggat cagcaggctg tagcgaacaa cctgcatcgg
    18901 gaagtactgc gcaaagtacg gacgccagat aatcaccccg gtcaccagca gcacgaaaat
    18961 catgctcatg atcgaccaga acatcatctt ttgcccggcg ttgtacttac cgacatccgc
    19021 cactttatgc tcattgcctt tcaatacttc gacaatgttc aacagccacg gaatatcttt
    19081 cttatccggg atgttgtgat gcacaaaacg cacaaacata aacatcagtg cgacgaaaat
    19141 cgcaatgccg aaaaacgggt gcaaaatgcg tcccatctgc ggcgtaccga aggtttgcgt
    19201 cagccattgc agcgtcggga agaagaacga aatcccggac agcgccacca ggaagaagca
    19261 aatcaccacg gtccagtgac aggcgcgatc aataaatttg gtgcgcacaa tcattttcga
    19321 cttactcatg atgatcctcc tcgtcatcgt ccacttcctt attcgggcca ataccgatgt
    19381 agtggaaaat caacccggca aaagtggcaa taaagccagc cgctgccagc ggtttcaacg
    19441 cgcctttcca caggcttacc gaggtgtcga tcttcggatc tttcggcaga ccgtgataca
    19501 gctccggctg atcggcgtga tgcagcacgt acataacgtg cgtaccaccg accccttccg
    19561 ggttgtagac gccagcatgt tcgtaaccac gcgctttcag tttcgccacg cgctgttccg
    19621 ccagctccag catctccttc ttggtgccga agtggatagc cccggtcgga caggttttca
    19681 cacaagccgg ttcctggccg acgctgacgc gatcgacgca gagcgtgcat ttatataccc
    19741 ggttatcctc tttgttgagg cgcggaatat taaacggaca cccggcaatg cagtaaccac
    19801 agccgatgca gttttccgac tggaaatcga caatcccgtt agcgtactga atgattgcac
    19861 cagcagacgg gcacgccttc aggcagccgg gatcttcaca gtgcatacag ccgtctttac
    19921 ggatcagcca ctccagcttg ccgttctgtt cggtttcgct aaagcgcatc accgtccagg
    19981 acttggcgct cagatcggcg gggttatcgt aaaccccgac gcagtgcccc acttcatcac
    20041 ggatgtcgtt ccactccgaa cacgccacct gacaggcttt acagccgata caggtggaaa
    20101 cgtcgataag ttttgcgact tctgctttgt aatcacgcac ctgagaaggc ggcgtgatgg
    20161 agttagttgc ggaccttttg ataatgtcct gcgtttccat agccatctgt tcgccccctt
    20221 acgccttctc gatgttgact aagaacgctt tatattccgg cgtttgcgag tttgcatcac
    20281 cgacattcgg cgtcagagtg ttagcgatat aacctttacg cgcgacaccc tcaaagcccc
    20341 agtggattgg aatacccacc gtttcaacct gctgaccatt tacattcagt ggtttcagac
    20401 gacgcgttac cacagccacc gcgcggataa agccacgctt gcttgagaca gtgacacgat
    20461 cgccattatt aatgcctttc gccgccgcca gcgtttcgct gatttccaca aactgttccg
    20521 gctgagcaat tgcgttgagc aatgcgtgct tggtccaggt gtggaagtgc tcggtcagac
    20581 gataggtcgt acccacatac gggaactgct cttttttacc catccgcagt gcgtcttgtt
    20641 catacagacg aacaaccggg ttagacacca cgttcggatg cagcgggtta gtgcccagcg
    20701 gcgtttcaat cggctcgtag tgttccggga acggaccttc cgccattttg ttgatggcaa
    20761 acaggcgtcc catcccttcc ggctgcatga taaacggccc ggttggcgta cccggtgcgg
    20821 cattgccgaa gtcaggaata tcgttacccg tccacttgct gccgttccac tggatcagca
    20881 tccgtttcgg atcccacggt ttaccgttga tatccgccga agcacggttg tacagcacgc
    20941 gacggttgag cggccacgcc caggcccatc ccagcgtatt ccccagaccg gacgggtcgg
    21001 agttatcgcg gttagccatc tggttgccct gctctgtcca gctaccggtg tagatccagc
    21061 aagaagatgc ggttgtaccg tcatcacgca gatgcgcaaa gctactcagc aactgacctt
    21121 tcttcgcaat cagcacgcca ttagcatcat agagatcttc cagcgcatag ccgttgttct
    21181 ctttagctac ttcgtcagat tgcggttcgt gcggctgctt gtagttccag ctcatcttca
    21241 tcagcggttc tacgccttta ccaccttcgg cctggtacag ctcgcgcagg tgatggtaga
    21301 tacccgccag aatttcaccg tcgttacgcg cttcgcccgg cgcatcctga cctttccagt
    21361 gccactgcag ccagcgaccg gagttagcaa tagaaccatc ttcttcagca aagcaggtcg
    21421 aaggcagacg gaatacttca gtctgaatag acgccggatc gacatcgttc gactcgccgt
    21481 ggttctgcca gaaggttgag gtttcagtca ccagcggatc gataaccacc atgtacttca
    21541 gcttgcttag gcagctcacc actttgtttt tgtccgggaa ggacgcaacc gggttaaagc
    21601 cctggcagaa ataaccggtg actttgcctt catccatcat gttgaaatac ttgatgacgt
    21661 cgtaggtctg gtcccacttc ggcagccagt catagcccca gttgttctct ttctgcgcgg
    21721 catcgccata gaaagatttc atcaggctaa cgaagaactt cggatagttg ctccagtagt
    21781 tcacctgacc agccagcgtc gctttcggcg tgttcgcttc cagatacgac tgcaaatcaa
    21841 cctgtttttc tgacggcagc gtcagataac ctggcaggct ggtagagagc agacctaagt
    21901 cagtcagacc ctgaatgttg gagtgaccac gcaatgcatt cacgccgcca ccggccatac
    21961 ccatgttacc gagcagcaac tggatcatcg ccatagtacg gatgttctgc gcacccacgg
    22021 tgtgctgcgt ccagcccagc gcgtacagga aggtggttgt gcgatccggt gcgctggtgg
    22081 aggccagcac ttcacacact ttcaggaagt cggcttttgg cgtaccacag atgttttcaa
    22141 caacgtccgg cgtgtagcgg gaaacgtgcg ctttcagcag gttccacaca cagcgcggat
    22201 gagtcagtgt ttcatcgcgt ttcgcatagc cgttttcatc gaactgatag ttccaggacg
    22261 atttatcgta ttgccgtttt tcagcgtcgt agccgctgaa cagaccgtct tcgaaagcaa
    22321 aatcatcacg caccagcagg ctggcgttgg tgtaatgctt aacgtattcg gcgttgattt
    22381 tgtcgttttc gatcaggtag cgcaaaacgc cagacaggaa cgtaatgtcc gtaccggaac
    22441 gaataggtgc gtaaatatcc gccacagaag cggtacgcgt aaaacgagga tcgacaacga
    22501 tcaaggttgc atcgttgttg tttttcgctt ccatcgccca gcggaaaccg acgggatgcg
    22561 cttcagcagc gttaccgccc attaccatta cgacgttagc gtttttgata tccacccagt
    22621 ggttggtcat cgcaccgcga ccaaatgttg gagcaagact tgctaccgtt ggtccgtgtc
    22681 agacgcgcgc ctggttgtct accgccagca tcccgaggga gcgggcaaat ttctgtgtca
    22741 gcatcccggt ttcgttgctg gcaccggagg cacacagcat accggtagaa agccaacggt
    22801 ttaccgttac gccctgctcg ttcttttcaa taaagttagc gtcacggtca gctttcatca
    22861 gctttgcaat acgggagaat gcttcttccc agctaatgcg ctgccatttg tcagaacctg
    22921 gcgcacgata ttccgggtag cgcagacggt tttcgctgtt gacgtaatcc agcaaaccgg
    22981 ccccttttgg gcacagcgca ccacggctta ccggatgatc cgggtcacct tcaatgtgat
    23041 aaatcgcttc tctggcgttt ttcgcgccat cacccaggct atacatcaat agcccgcaac
    23101 ctacggaaca gtatgtgcag gtgttacgga tctctttagc gcgtaataat ttgtagtttc
    23161 gcgcctgagc cagtgcttgc ttcggggcaa agcccaatgc cgctaccgtt gttccagcca
    23221 taccgcccgc gcagatttta aaaaattgtc tgcgactgac gtccattgct ttcctctttt
    23281 ttcaggggta ttactgcgcg aggaaactaa cagtaaaatt ctgaatcttt ttgcgcgaaa
    23341 tcaaaaagaa cacgtgtttg ccacaaaata atccgcctgc gcaaattgac ctacctcaat
    23401 agcggtagaa aaacgcacca ctgcctgaca ggccagttaa aaaaatgcta taaaattcag
    23461 cttaattttt aacggcaaga gagacaaaac agcgagcatg acacgacaaa aagcaacgct
    23521 catagggcta atagcgatcg tcctgtggag cacgatggta ggattgattc gcggtgtcag
    23581 cgaggggctc ggcccggtcg gcggcgcagc tgctatctat tcattaagcg ggctgctgtt
    23641 aatcttcacg gttggatttc cgcgtattcg gcagattccg aaaggctatt tactcgccgg
    23701 gagtctgtta ttcgtcagct atgaaatctg tctggcgctt tccttagggt atgcggcgac
    23761 ccgtcatcag gcgattgaag tgggtatggt gaactatctg tggcccagcc tgacaattct
    23821 ctttgccatt ctgtttaatg gtcagaaaac caactggctg attgtaccag gattattatt
    23881 agccctggtc ggcgtctgct gggtgttagg cggtgagaat gggttacatt atgatgaaat
    23941 catcaataat atcaccacca gcccattgag ttatttcctg gcgtttattg gtgcgtttat
    24001 ctgggcagcc tattgcacag taacgaataa atacgcacgc ggatttaacg gaattaccgt
    24061 ttttgtcctg ctaacgggag caagtctgtg ggtttactat tttctcacgc cacaaccaga
    24121 aatggtattt agcacgcccg tcatgattaa actcatctct gcggcattta ccttaggatt
    24181 tgcttatgct gcatggaatg tcggtatact gcatggcaat gtcaccataa tggcggtagg
    24241 ctcgtatttt acgcccgtac tttcctcagc gcttgcagcc gtgctgctca gcgccccgct
    24301 gtcgttctca ttctggcaag gcgcgctaat ggtctgcggc ggttccctgc tctgctggct
    24361 ggcgacacgt cgtggttaaa ataattgccg ggtctacggc ccggcagtta atcagataaa
    24421 ttccgacaat attaaaaaat aaatttcaaa taccagtaat tcaccttata tctcatttcg
    24481 ac
//
LOCUS       DAZFWL020000065        23832 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFWL020000065 DAZFWL020000000
VERSION     DAZFWL020000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.065"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..289
                     /locus_tag="ACHKNP_RS13765"
                     /old_locus_tag="pgaptmp_002753"
     CDS             <1..289
                     /locus_tag="ACHKNP_RS13765"
                     /old_locus_tag="pgaptmp_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4704892.1"
                     /translation="SVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQAEIS
                     WRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(332..1483)
                     /gene="ydiO"
                     /locus_tag="ACHKNP_RS13770"
                     /old_locus_tag="pgaptmp_002754"
     CDS             complement(332..1483)
                     /gene="ydiO"
                     /locus_tag="ACHKNP_RS13770"
                     /old_locus_tag="pgaptmp_002754"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HGU4704893.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1497..3091)
                     /gene="ydiF"
                     /locus_tag="ACHKNP_RS13775"
                     /old_locus_tag="pgaptmp_002755"
                     /pseudo
     CDS             complement(1497..3091)
                     /gene="ydiF"
                     /locus_tag="ACHKNP_RS13775"
                     /old_locus_tag="pgaptmp_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
     gene            complement(3235..3993)
                     /gene="aroD"
                     /locus_tag="ACHKNP_RS13780"
                     /old_locus_tag="pgaptmp_002756"
     CDS             complement(3235..3993)
                     /gene="aroD"
                     /locus_tag="ACHKNP_RS13780"
                     /old_locus_tag="pgaptmp_002756"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGU4704894.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4024..4890)
                     /gene="ydiB"
                     /locus_tag="ACHKNP_RS13785"
                     /old_locus_tag="pgaptmp_002757"
     CDS             complement(4024..4890)
                     /gene="ydiB"
                     /locus_tag="ACHKNP_RS13785"
                     /old_locus_tag="pgaptmp_002757"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HGU4704895.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4902..6167)
                     /gene="ydiN"
                     /locus_tag="ACHKNP_RS13790"
                     /old_locus_tag="pgaptmp_002758"
     CDS             complement(4902..6167)
                     /gene="ydiN"
                     /locus_tag="ACHKNP_RS13790"
                     /old_locus_tag="pgaptmp_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4704896.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6394..7608)
                     /gene="ydiM"
                     /locus_tag="ACHKNP_RS13795"
                     /old_locus_tag="pgaptmp_002759"
     CDS             complement(6394..7608)
                     /gene="ydiM"
                     /locus_tag="ACHKNP_RS13795"
                     /old_locus_tag="pgaptmp_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4704897.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVDSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7708..8064)
                     /gene="ydiL"
                     /locus_tag="ACHKNP_RS13800"
                     /old_locus_tag="pgaptmp_002760"
     CDS             complement(7708..8064)
                     /gene="ydiL"
                     /locus_tag="ACHKNP_RS13800"
                     /old_locus_tag="pgaptmp_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HGU4704898.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8200..8307)
                     /gene="rprA"
                     /locus_tag="ACHKNP_RS13805"
                     /old_locus_tag="pgaptmp_002761"
     ncRNA           complement(8200..8307)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKNP_RS13805"
                     /old_locus_tag="pgaptmp_002761"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8493..9605)
                     /gene="ydiK"
                     /locus_tag="ACHKNP_RS13810"
                     /old_locus_tag="pgaptmp_002762"
     CDS             complement(8493..9605)
                     /gene="ydiK"
                     /locus_tag="ACHKNP_RS13810"
                     /old_locus_tag="pgaptmp_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HGU4704899.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            9994..13050
                     /gene="ydiJ"
                     /locus_tag="ACHKNP_RS13815"
                     /old_locus_tag="pgaptmp_002763"
     CDS             9994..13050
                     /gene="ydiJ"
                     /locus_tag="ACHKNP_RS13815"
                     /old_locus_tag="pgaptmp_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HGU4704900.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13047..13457
                     /gene="menI"
                     /locus_tag="ACHKNP_RS13820"
                     /old_locus_tag="pgaptmp_002764"
     CDS             13047..13457
                     /gene="menI"
                     /locus_tag="ACHKNP_RS13820"
                     /old_locus_tag="pgaptmp_002764"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HGU4704901.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13557..13745
                     /gene="ydiH"
                     /locus_tag="ACHKNP_RS13825"
                     /old_locus_tag="pgaptmp_002765"
     CDS             13557..13745
                     /gene="ydiH"
                     /locus_tag="ACHKNP_RS13825"
                     /old_locus_tag="pgaptmp_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HGU4704902.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14293..14661
                     /gene="sufA"
                     /locus_tag="ACHKNP_RS13830"
                     /old_locus_tag="pgaptmp_002766"
     CDS             14293..14661
                     /gene="sufA"
                     /locus_tag="ACHKNP_RS13830"
                     /old_locus_tag="pgaptmp_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HGU4704903.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14670..16157
                     /gene="sufB"
                     /locus_tag="ACHKNP_RS13835"
                     /old_locus_tag="pgaptmp_002767"
     CDS             14670..16157
                     /gene="sufB"
                     /locus_tag="ACHKNP_RS13835"
                     /old_locus_tag="pgaptmp_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGU4704904.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16167..16913
                     /gene="sufC"
                     /locus_tag="ACHKNP_RS13840"
                     /old_locus_tag="pgaptmp_002768"
     CDS             16167..16913
                     /gene="sufC"
                     /locus_tag="ACHKNP_RS13840"
                     /old_locus_tag="pgaptmp_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGU4704905.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYRGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16888..18159
                     /gene="sufD"
                     /locus_tag="ACHKNP_RS13845"
                     /old_locus_tag="pgaptmp_002769"
     CDS             16888..18159
                     /gene="sufD"
                     /locus_tag="ACHKNP_RS13845"
                     /old_locus_tag="pgaptmp_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGU4704906.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18156..19376
                     /gene="sufS"
                     /locus_tag="ACHKNP_RS13850"
                     /old_locus_tag="pgaptmp_002770"
     CDS             18156..19376
                     /gene="sufS"
                     /locus_tag="ACHKNP_RS13850"
                     /old_locus_tag="pgaptmp_002770"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HGU4704907.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19389..19805
                     /gene="sufE"
                     /locus_tag="ACHKNP_RS13855"
                     /old_locus_tag="pgaptmp_002771"
     CDS             19389..19805
                     /gene="sufE"
                     /locus_tag="ACHKNP_RS13855"
                     /old_locus_tag="pgaptmp_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HGU4704908.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19954..20958
                     /gene="ldtE"
                     /locus_tag="ACHKNP_RS13860"
                     /old_locus_tag="pgaptmp_002772"
     CDS             19954..20958
                     /gene="ldtE"
                     /locus_tag="ACHKNP_RS13860"
                     /old_locus_tag="pgaptmp_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HGU4704909.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21021..21257)
                     /gene="lpp"
                     /locus_tag="ACHKNP_RS13865"
                     /old_locus_tag="pgaptmp_002773"
     CDS             complement(21021..21257)
                     /gene="lpp"
                     /locus_tag="ACHKNP_RS13865"
                     /old_locus_tag="pgaptmp_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HGU4704910.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(21568..22980)
                     /gene="pykF"
                     /locus_tag="ACHKNP_RS13870"
                     /old_locus_tag="pgaptmp_002774"
     CDS             complement(21568..22980)
                     /gene="pykF"
                     /locus_tag="ACHKNP_RS13870"
                     /old_locus_tag="pgaptmp_002774"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HGU4704911.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(22988..23182)
                     /locus_tag="ACHKNP_RS13875"
                     /old_locus_tag="pgaptmp_002775"
     CDS             complement(22988..23182)
                     /locus_tag="ACHKNP_RS13875"
                     /old_locus_tag="pgaptmp_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704912.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            23537..23746
                     /gene="fumD"
                     /locus_tag="ACHKNP_RS13880"
                     /old_locus_tag="pgaptmp_002776"
     CDS             23537..23746
                     /gene="fumD"
                     /locus_tag="ACHKNP_RS13880"
                     /old_locus_tag="pgaptmp_002776"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HGU4704913.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         6202 a         5738 c         6006 g         5886 t
ORIGIN      
        1 cagcgtcagt tatatttgcc atgaatttac caaagagtat cgtatttccc ctattaacta
       61 tgttattcaa cggcgtatga cggaagcaaa atggtcactc actaatactg aattatcaca
      121 ggcagagatc tcctggcgtg tgggttatga aaatgtcgat cactttgcca aactgttttt
      181 gcgccatgtc ggttgttcgc ccagcgatta ccgtagacaa tttaaaaact gttttgcgga
      241 acaagaaatc ctatctgaat ttcctcaacc ggtaagtctt gttggataaa tctggcggcg
      301 cagtttgtta agaagctgcg ccgcctgcag attatttgtt ctgatagtct ttcaggatct
      361 gccgacctgc tacgtaaatc ataatttcgt ctgtaccgcc gccgatacgt tcacaacgga
      421 catcacgcca gaagcgggag acgcgcgctt catcggtata gcccagaccg cccatgattt
      481 gaatcgcatc atcaatgact tccattgcgg tacgtgcgca atacagtttt gccagcgccg
      541 cgctggtgcg cagtgactga tgctgatcgg cttgccatgc cactttcagc accatgttgc
      601 gcatgttgtc gatcttaatc gccatcagcg ccagtttttc ctggatcatc tggttatgac
      661 caatgggctt accaaaagcg atacgttggt tggcatagcg ggcggcatct tcaaaggcgc
      721 attcggcaaa gccggtgctg cgcgcggcgt tgatcaggcg ctccatctca aagttgtaca
      781 tcacattgag gaaacccatt ccttcttcgc ccaccatgtc gctctcttca acttccacat
      841 tgtcgagata gacttcgcag gtgctgagca tatgccagcc gattttatgc agcgggttaa
      901 tcttaatgcc gggcttactg gagtcgaccc accacagggt gaaggctttt ttgggatctt
      961 tcggttgcgg atcgcgcgcc agcaccagca tatacgggta ctctttcgca ccggtaataa
     1021 aggttttttg tccgttgatg tagaccttgc cgtttttacg cgtgtaagtg gtagtggcac
     1081 tgttgttatc tgagccagca cctggttccg tcaacgccag ggcataggcg ggatcacccg
     1141 tttccagggt gctctctgcc gttttacgta gctgctctgc agaaccgaaa cggcgcatac
     1201 tgtgaataca ttgaccgttg gtaattaaaa atgccggagc accgcatttt gacacttcca
     1261 tcagcgccag catttgggtg acgtaatccg cagggatacc accaaattct tccggcacgc
     1321 caagcatgga aataccgtta tccgccagcg cccgcataaa ctcacgcgga tatgtcccgt
     1381 tttgatcgca ggtgcggaaa tactcttccg gaaagttagt cgtaatcagt tcgcgaatac
     1441 tggccagcag cagttcttgt tcttcagtta aagaaaaatc catcattata ctcctgttaa
     1501 tgagccgctt caggcaggac aaaacccatc gccgcatcga taaataatct ttcgtccatc
     1561 agtttgagtt ctggcgaaat cactggggtg aagtccattt tgtcgagaat atctttttgt
     1621 aaatcgacgc caggggcgat ttcgattaaa tgcaggccgt cttctttcag cgtgaataca
     1681 gcacgttcgg tgatgtaacg aacatccagt ccccgttcga gcgcgatttt tccgctaaaa
     1741 gtaatttccg gtagttcccg aataaattta tttacccgtc cttcctggac gatatgcaat
     1801 ttgccatcgg caatttctgt ttttaaactg cccgcagtta acgtgccgca gaaaataatt
     1861 ttcttcgagg tggcactgat atcaataaat ccaccggtac ccatgatttt gccattgaat
     1921 ttatgcacgc cgacgttacc gtgctggtcg acttcagcaa aactcaaata acaaacatcc
     1981 agaccaccac cgtgataaaa atcaaactgg gacgtcatat ccagaatggc gcgggtattc
     2041 acgttcgcgc caaaggcgat accctgtgaa gtaattccgc caatcggacc tgtttctacc
     2101 gtcagaataa agtcatcagc acaaccttct tcccgggcga ccaggccaat accgtcagca
     2161 ataccgacgc cgacattccc caccgcgcct ttacgcattt cgaataatgc acgccgcgca
     2221 actaatttac gttggtttag gggcaggcta agtttgtgct gtcatcaagg gtgaagtcac
     2281 cagaaataaa acggttaacc ggcgcaccgc catacagttg gctttgatcc ggatcgacca
     2341 ccacaatatc caccagataa cccggaatac ggacagattt aggatgcagc gtggctttct
     2401 taaccatttt ctgcacctgc atcatcacaa taccgccgtt attgtgcacc gcctgggcaa
     2461 taaccaatgc gtcgagatac atcacctcat cttcaaaagt ggcgtagcct tcactgtcgc
     2521 aggtggtagc gcgaatgaag gcgatatctg gcgcaatcgc tttgtaatag agatattctt
     2581 tgttatcaaa ctcgaccagt ttaatcaggt cttctttcgt gacttcattc agtttgccgc
     2641 cttgctggcg tggatcgaca aatgtcccga tgccaatatc actaataata ccaggctggt
     2701 gggctgcggc ggcgcgtaag gtttgtgtaa gtacaccttg tggatagtta taagcaataa
     2761 ttttattttg ttctgcgagt tcagaaatac gcggcgattg tccccagtga ccacataatg
     2821 cccatttcac cagaccttct tgcgccagag gactaatacc acggtcggcg cgatcgccaa
     2881 gccctgtcgg actaataatc gataaattac gtggcgtttg agtctgttta tatttatcag
     2941 caagagctgt aattaacgtg gtggcttcca gaataccgcc gccagcgcct aacacacaaa
     3001 gtgttgcttc gtcgggaata taattcaccg cttcctgtgc cgacaggacc ggcactcgtc
     3061 cattaatacg aggtggtttt acaggtttca ttgttaattc ctgattcaga tgtgattttc
     3121 cctacgccag atggagaatt agcgcacaga gactcacgaa atatttttat taaatacggt
     3181 taaatatatt ttttaagttc ggcggggagg gtgttcccgc cgaaatatta ttgcttatgc
     3241 ctggtgtaaa atagttaata ccgtgcgtaa atcatttacc gagatttgcc ctggtgcaga
     3301 ggcttttttt accgcaccaa aagttgccgc cgagccaaat acttcaccag ccagacgaga
     3361 aattacgcca gtttttgcca tcgacatcgt gatgattgga cgatcggcat actgctcctg
     3421 catctccagg gtcgcggtaa gcaacgtcag cacatcgctg gtactttgcg gcatcagcgc
     3481 aatcttagga atatcggcgt cgaaggactg cattttgcgc agacgggcaa tgatttcttc
     3541 ggcttccggc gttttatgga agtcatggtt ggacatgaca actttcacat catgcgcgtg
     3601 ggcgtaggcg acggtttctt taacctgatc atcgccggta aataactcca gatcgatcat
     3661 atcaaccagg ccgctgtcga tggctgcacg attgagagca atataagcct cggtggaaat
     3721 cgcctgctcg ccgccttctt tggcactgcg gaaggtaaac agcagcggtt tttctggcat
     3781 ggtttcacgg agaatttttg ccgccgccat gacagactcc acattggaga ggtcggcaaa
     3841 gtggtccaca cgccattcca gaatatcaaa gtccgcttca cgataggcga gagcttcgga
     3901 tttcacgcgg gcgatatctt tcgccatcag cgagacgatg attttaggtg cgcccgtacc
     3961 aatgacgaga tcttttacag ttacggtttt cattttttac cctttctgca cgcggtcagc
     4021 ctgtcaggca ccgaacccca tgacctgttt aacatattcc agagggaaat ctttgccagt
     4081 ccataacgtg aactgttcag ccccttgcca caacaacatg ccgtatccat caatcgtttt
     4141 gcaaccagct tgttgcgcct gctgcaataa cttcgtcata tgcgggttat acacgcattc
     4201 agtgaccagc agtcccggat gtaacagact gatatcatta actaatgatt cattctcaag
     4261 gggtttcata cccacttttg tgccattggt taaaatgtcg gcggaagcca gggcttcagc
     4321 aaaggcttgc tgatcggcga gatcggtaac cgtgacgaca caatcggtgt tttcgttaac
     4381 ccgctgcgcg aaggcgaggg ctttatcgaa gaactcatcc cgacggttaa agagtttaat
     4441 ttcttttaaa ccttcaattg ccccctgcgc gccaatcgcc gttgaggcac ctccggcccc
     4501 taacagcacc attgttttgc ctttgatatc aaaaccgctc tctttaatgg cgcgaatatg
     4561 gcccgtgccg tcggtgttat agccgcgcag gtagccatca tcattaacga tggtgttgat
     4621 ggcacccacc agtttggctg ctggcgttaa ttcatcaaca tattcacacg ccagttgttt
     4681 gttcggcatt gatataccag ttccgcgcat tttgagggct tttaatcctt caattgctcc
     4741 gggaaaacta tcgttatcca cttcaaaggc catataggta aatggcaatc ccgctttttc
     4801 taaggcttta ttctgcattt caggcgataa actgtggcgg ataggatagg ccatcagccc
     4861 aatcaattcg tattttgcgg taacatccat aattaactcc tttaacctct atgcttaatt
     4921 gtgtttaacc gggtgctaaa tttacgctcg ccaaaccgca catcattctc aggaataata
     4981 aaaacgcggt aatagcggat aaaaacaata attgcggtaa tcagggccag cagcgcgaaa
     5041 gtaaaatcga gaacaatgat atattgaagg ccgatgttcg acagataacc ggtaatcagc
     5101 ggaataacaa agttagccag tccacccatc atcatataaa tactggtgac tttggctttg
     5161 ctttttggga aaaactctga catgaccgaa acaccgagct gtaaaatgcc gccagctgct
     5221 gagaaaccga taacaaaggc gccggcatta cacaccagtg gagaagggta caggtagata
     5281 atggctgctg ttattgttgc cagtgcagag ttaaatacat tagcccagat gggccggacc
     5341 atttttttca gtagtgcggc aaaaataaag acacagacca acgagcccat actgtaataa
     5401 gagatggttt ttaatgcctc agcttctgac ataccagcaa aagccatcgc atatttgggc
     5461 atccacacca caatcacata aaaggtcgag aatgcggcta caccgaacag taccgatgaa
     5521 acaccttcca gccagactaa cggtttgctg ttcatttgcg gtaattcatt agctacgctg
     5581 gcgtccacca actggctggg gaatttgctt ttcaacagca tcagcgtgat cagtacaaac
     5641 agaatacccg gaataatcag cccatagccg taccagatat tattgagcaa catatagctc
     5701 accagcattg ggtagaacat ttgcccaaat gacaccatcg ctttaaccag tatgaccgcc
     5761 gaaccagagg ctttcggaaa gcattccatg agcgcggggt agccacccgt atccagcgct
     5821 gagttagcga tacctacgca cactgccaga ccgtaggcga gagttaaatt cgggcaagcg
     5881 ggaataccaa agaagaatag cagatacatt attactgcca ttaatatcac cgcccgacga
     5941 ccaaacttat cggagatcac accgaagaat aaaatactga tcaatcgccc caaaccaata
     6001 ccggaaatta agtaggcaat gcccgcgttg tcagtggaaa acttttccgc cagagatgac
     6061 atgttttggg caagcgtaat aacactaatg ccgtgcagga agtagctgaa gtaaatacaa
     6121 agaacagcca ggataaatgg cgtgctgaaa gccttatttt gagacatttt cacagctcct
     6181 tgctgaaaga taatttcgtt accgtacgta ttgttcttcg acggtttaca taattactat
     6241 actgaagatg attttccctg ggtacatggc ataaagttta tgtttccagg aatattgcat
     6301 gtcatagttt gtttttgatc gcgattttcc ttagttagcg cagaaagagt tatttatccg
     6361 taagatcttg catgacctga aaagaatatt gcattaccca cccggagcga cattttcctt
     6421 tagcgagtga tgccgcgttt ttttgcggct gcgtaagccg ataaacagtg ccagtaaaaa
     6481 accgatggca gcgatggcgg tatcgaacca cataatatcg gcaatacttc tttgggacag
     6541 atgagccgta atcaacggaa tagtaaaggt cgcaatactg cccgcactgt aatagatccc
     6601 tgtagcttta cctttagcgt aagggaaacg ttcagccatt aacgtcaggc caatttgcac
     6661 aacacctcca gcagaggtaa aaccaatgac aaaagcaaat attatcacca cataaaatgt
     6721 gggatgcagg cagacggtaa acagagcaat aaatgagata aaggtgtaca gcatcagtaa
     6781 tgttgttggg cgaacggtat tacgaatgag tggagcggta ataaatacac aaagcagcga
     6841 atccacggtg tagatactga gtagtttaat cgacatagtg tatgacatgc ctgcaacaaa
     6901 ttgtccgtac tgtgccagcc actggctaac cagataaaac gttgccattg agatatagcc
     6961 atataaggta taactggcta aatcgataat tgaacagcga tgttccgtag agctggtggt
     7021 tttctttatg acaggtaagc gatgacccgg atggggtggg aacgtacaac ggtataaaaa
     7081 cagagcgtta ataaacataa tgcctgcagc aatcataaag gaccaaccga accacagttc
     7141 agcccacact aacaggctaa tgattagcgg taataaaaat tgtccgctgg aaacaaatgc
     7201 tttaattaaa atattggctg tcccaggtga gcgtggaaaa gcttccatca aactgggata
     7261 agtgcctgca tcgagaaaac tgtttgccat tcctgccaga aagccaaaaa cataagcgat
     7321 aatgatgtta ttggtgtgca ggatgccaaa aaagaaggcc atatagcagc acatcccgag
     7381 catgataaaa gggcggcgac caaagcgatc ggataataat cctgcaaaaa gcaagacact
     7441 taatcgacca atgcccagcg atgagataac tatcgagaca cccgcggcat tagtctgcca
     7501 aagtgtctcc agcgaggcca tattcaggct catcaaaatg acgcccatac catgcactag
     7561 gtaattaaaa tacaacccaa gtgcggtagg gtaataggga tttttcatag gttctactta
     7621 gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta tccgttcatt attgttgtaa
     7681 caacgacaat tcctttttgt ggagaaatta acaaagccgc tccagatcgt gtgcatataa
     7741 ttcagcggct accgattgat agatcttcca gtcaataaaa ttgccatcag ggtagacttg
     7801 ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg ttgccgatgc ggttatttat
     7861 tttctctatg atagcatgaa gatgtttttt tcgttgctga cgcatagcca acagttgctc
     7921 gactacacga tcaggaatgg cgcatttgcc attttcccac tgacgccagc tttcactgtc
     7981 accggtctgg gcaatccagg tcgcgcattc atcaatagtc atggcaaaaa tatggcgaag
     8041 tgcctggagt tcataagcgt tcatcgtgta atggctgcat gaaataaaca ataaaatata
     8101 aataacatta tcagcctgct gacggcttga agagagtcac agtatcttgt gcaacattat
     8161 tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt taaaaaaaag cccatcgtgg
     8221 gagatgggca aagactacac acagcaattc gttgtttcac tcaggggatt tccatgctta
     8281 taaatcaata tgttgattta taaccgtaag ctaatagtag gcatacggac tttttgcgtc
     8341 gatcagattc gtctcattag tttaaaaact gtaaagaatt tgcgacaggt actaccagct
     8401 gaatggcggt aagcaggaaa atatggggaa gagcaagcaa atatcaatta gcgggggtta
     8461 acaatcaggc ggcagagccg cctgatgaga aattacttat tcggtttctc gatttcgcca
     8521 agttcttcga gaatttcttc cggttggtcc gtcggcggcg ggacttcttc cacccacgcg
     8581 gcaaacagac gccaggaaac ggctaacaga accggaccaa taaacagacc gatcatcccg
     8641 aaagcaatca aaccaccaat aacaccagag aggatcagga tcagcggtaa atcggcaccc
     8701 atgcgaatta acattgggcg gatgacgtta tccagcgtgc caaccacacc gctccacact
     8761 aacaataccg ttccccaggt ggtatcgcca gtccagtaga gccagataat cgccggaatc
     8821 agtaccggca acgggccaag ctggacaagg caggagagga tcattaacac cgttagcaac
     8881 gttgcataag gtacgccgga tacggcgagg ccgataccgc caagcactgc ctgtactaac
     8941 gccgtcacca ccacacccag cgccaccgcg cggatagcct gtgccgccag cagtacggcg
     9001 gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc gaatgccttg tgccacctgt
     9061 tcaccgcgcc agtacagcag ggcactgaag agaagcatca gcgcacaatg caccataaag
     9121 cgcccgatat gcgccgcctg cccaacgaac caggtggtgg tggtgccaat ataagggcgg
     9181 actttcgcca tgatcgccgt cccccccata tccagcaagt tgtgccagcc tgcatacagc
     9241 ttcgcgccaa tcaccggaat ggtattaagc cacgccagat cgggtaacgt catgtcgccg
     9301 ctggaaatgg ctttaattag cgggccgctg ccgtcgacga tactgttaac cagcaaagca
     9361 atagggatga taaacaccat cactaataac agcgtcatca ccagaacggc gagagagcgg
     9421 cggccaaaca tgatcttttg caaacgtaac aataccggcc aggtggcgat aaccaccgta
     9481 ccggcccatg caaagccgag aataaagggt tgaacaatcc acagacatgc cacaatcatg
     9541 atggctaaaa acagcaccga aagcagaatt tgtgcgacat ccctgggctg acgaacattt
     9601 accataacta cttttcacct tttttgtgcg tcaaatcgtc gacgcgacgt gaacacgcga
     9661 aactcacctg cataatgatg agcaatttca gcggttttta acaggccgat tctgccctta
     9721 attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac tcaggcaaca cgcaaacgat
     9781 ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt tcgttcaccc cagtcactta
     9841 ctattgtaag ctcctggagt tcactcgctt gccgccttcc tgcaactcga aatccataga
     9901 gtaaataaac atcacctttc agtgcgctat tgcagccgcc tgaaagaata gtgtatcgac
     9961 ctacatcaca gaccgcagga aagggtcaat ataatgattc cacagatttc ccaggcaccc
    10021 ggcgtcgttc aactggtgct taattttttg caagagctgg agcaacaagg ttttaccggc
    10081 gatacggcga caagttatgc cgatcgtctg acaatgtcga ccgacaacag tatttaccaa
    10141 cttctccccg atgcggtggt atttccgcgt tcaaccgcag atgtggcgct gatcgcccgt
    10201 cttgccgcgc aggaacgcta ttcatcgctg atctttaccc cccgtggcgg cggcaccggc
    10261 actaacggtc aggcgctcaa ccaggggatt attgttgata tgtcccgcca tatgaaccgc
    10321 atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg aggccggggt gataaaagat
    10381 caactcaatc agtacctgaa accgttcggc tacttttttg cgccggaact ttcgaccagc
    10441 aaccgggcaa cgctcggtgg gatgatcaat accgatgctt ctggtcaggg atcgctggtc
    10501 tatggcaaaa cgtcggatca cgtacttggc gtacgtgcgg tgttgttggg gggcgatatt
    10561 ctcgatacgc aacctttacc cgtcgaactg gcggaaacgc tgggtaaatc caataccaca
    10621 atcgggcgaa tttataacac ggtttatcaa cgttgccgtc agcaacgcca gttaatcatc
    10681 gacaacttcc ccaaacttaa ccgctttctt accggttacg atctgcgtca tgtctttaac
    10741 gatgagatga ccgagttcga cctgacgcgc attctgacgg gttcagaagg gacgctggcc
    10801 tttattaccg aagcgcggct ggatattacg cccttgccta aagtgcgccg tctggtgaac
    10861 gtcaaatatg actcttttga ctccgcgctg cgtaacgcgc cgtttatggt tgaggcgcgg
    10921 gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc tggcgcggga agatattgtc
    10981 tggcattccg tcagcgagtt gattaccgat gtgcctgaca aagagatgct cgggctgaac
    11041 attgtggagt ttgctggtga tgatgaggcg ctgattgacg agcgagttaa tgctctctgt
    11101 gtgcggcttg atgagctgat tgccagccaa caggcaggtg tgattggctg gcaggtgtgc
    11161 cgcgagctgg cgggtgttga acgtatctat gcgatgcgta aaaaagccgt tggtctgctt
    11221 ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg aggatacctg cgtaccgccg
    11281 gaacatctgg ccgattatat tgctgaattt cgcgcgctgc tcgacagcca cggcttaagc
    11341 tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg tccgtccagc gctggatatg
    11401 tgcgatcctc aacaagagat tttgatgaag caaatctctg atgacgtagt ggcgctgact
    11461 gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag gttttcgcgc tgaatacagc
    11521 ccggcgtttt tcggtgagga actttttgca gaactgcgca aagtgaaagc ggcatttgac
    11581 ccgcataacc gactcaaccc agggaagatt tgcccgccag aaggtctcga tgcgccgatg
    11641 atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc agatccccat tgcggtacgc
    11701 cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt tatgcttcaa ctttgatgcc
    11761 cgtagtccga tgtgtccgtc gatgaagatc acccagaacc ggattcattc accgaaaggg
    11821 cgcgcaacgc tggtgcgtga atggctgcgt ttgttggcgg atcgcggcgt tgatccactc
    11881 aaactggaac aagaactgcc tgaatccggc gtcagtttgc ggacgttaat tgcccgcacg
    11941 cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct cacacgaagt caaagaggcg
    12001 atgtcgggct gtctggcctg taaagcgtgt tcgacccagt gccccatcaa aattgatgtg
    12061 ccggagtttc gctctcgttt tctgcagctc tatcacaccc gttatttacg cccgctgcgc
    12121 gaccacctcg tcgctacggt cgagagctac gcgccgctga tggcacgcgc gccgaaaacc
    12181 tttaacttct tcattaacca gccgctggtg cgcaaactct cggaaaaaca tatcggcatg
    12241 gttgatttgc cgctgctgtc ggtcccctcg ctacaacaac aaatggtggg gcatcgctcg
    12301 gcaaacatga cgctggaaca gcttgaatct ctcaatgcag agcagaaagc gcgcacagtg
    12361 ttggtggtgc aggacccctt taccagctat tacgatgcgc aagtggtggc ggattttgtc
    12421 cgtctggtcg aaaaattagg tttccagcct gtgttactgc cattttcgcc aaatggcaaa
    12481 gcccagcata ttaaaggttt tcttaatcgt tttgcgaaga cggcgaaaaa gacggcggat
    12541 ttcctcaacc gtatggcgaa gctgggtatg ccaatggtgg gcgtcgatcc ggcgctggta
    12601 ctttgttatc gcgatgaata taaactggcg ctgggcgagg aacgtggcga gtttaacgtc
    12661 ttactggcga atgaatggct ggcaagcgca cttgagtcac agccagtggc tacagtcagc
    12721 ggtgaatcat ggtatttctt tggtcactgt accgaagtta ccgccttgcc gggtgcgcca
    12781 gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac tggaaaatgt cagcgtgggt
    12841 tgctgcggca tggcagggac ttacggacat gaagcgaaaa accataaaaa ttcgctcggg
    12901 atctatgagt tatcctggca tcaggctatg cagcgactgc cgcgtaaccg ctgtctggcg
    12961 accggatatt cctgccgtag ccaggtaaaa cgggttgaag gcacgggggt acgccatcct
    13021 gtgcaggctt tactggagat tattaaatga tatggaaacg gaaaatcacc ctggaagcac
    13081 tgaatgctat gggtgaagga aacatggtgg ggttcctgga tattcgcttt gaacatattg
    13141 gtgatgacac ccttgaagcg acaatgccag tagactcgcg gacaaagcag cctttcgggt
    13201 tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat cggttccgtt gccggttatt
    13261 tatgtaccga aggtgagcaa aaagtggttg gtctggaaat caatgctaac cacgtccgct
    13321 cggcacgaga agggcgggtg cgcggggtat gcaaaccgtt gcatctcggt tcgcgtcacc
    13381 aggtctggca gattgaaatc ttcgatgaga aagggcgttt gtgctgttcg tcacgattga
    13441 cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac gcatattgcc tttggtcatt
    13501 atctgcacag taaacggtgg tatactgtgc aggtcttgtc taaaagcgag gatgctatgt
    13561 ctactcaact tgatcccacc cagctggcca ttgaattttt acgccgtgac cagagcaatc
    13621 tttcacctgc ccaatatctc aaacgtctga aacaactaga acttgaattt gctgacctgc
    13681 ttactctctc ttctgctgaa ttaaaagaag aaatctactt tgcctggcgt ctgggcgtgc
    13741 attgatcctg acttaatgcc gcaagttcgc caaatcgacc tggattaacc tccgtccagg
    13801 ttcgatgacc ttcttaatac tgcttatcta ccgcctgata ttgttcaaaa ataatactaa
    13861 tcgcagtttg tgttaaaacg gcgggttagc tttatgagat tattcttatc gccccttcaa
    13921 gagctaagcc actgagagtg ccggagataa gcgccggatg gggtagaaac ccttaagcct
    13981 gtgttgcaca gacttaaggg ttttcttatt tctgaacgct gcactttttt acctatctct
    14041 taaatagtca ttttcaatac aactttttct ttttcatcac agccttaatg ctccttagat
    14101 aataaccatt atctaacaat gagataccta attcttagcg tgcctgttaa cccacacatc
    14161 agggtctatg cttattaaaa gcaggcaaaa agcatgtttt acggtaaagc ccctgcgttt
    14221 gctgggttgt actgataatc attatcacta acatgctgtt atacgctgaa agcgatgaag
    14281 tgaggtaaat cgatggacat gcattcagga acctttaacc cacaagattt cgcctggcaa
    14341 ggcttaacgc tgacacccgc agcggcgata cacatccgtg agctggtggc aaagcagccg
    14401 ggtatggtcg gcgtgcgctt aggcgtgaag caaacgggct gcgcgggctt tggctatgtg
    14461 ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt ttgaacacga cggcgcgaag
    14521 ctgtttgtcc cgctgcaagc gatgccgttt attgatggca cggaagtcga tttcgttcgt
    14581 gaaggactta atcagatatt caaatttcac aaccctaaag cccagaatga atgtggctgt
    14641 ggcgaaagct ttggggtata ggcggtacta tgtctcgtaa tactgaagca actgacgatg
    14701 tcaaaacctg gaccggcggc ccgctgaatt ataaagaagg attcttcacc cagttagcca
    14761 ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg cgcaatttcg gcgaagcgta
    14821 atgagccgga gtggatgctg aagtttcgtc taaacgccta tcgcgcatgg ctggagatgg
    14881 aagaaccgca ctggttgaaa gcgcactacg acaagctgaa ttatcaggat tacagctact
    14941 actcagcacc atcgtgcggt aattgtgacg acacttgcgc gtctgaacct ggcgcggtgc
    15001 agcaaactgg cgcgaacgcc tttttaagta aagaggtgga ggcggcgttt gagcagttgg
    15061 gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc cattttcgac tcagtttcgg
    15121 ttgccactac ttatcgcgaa aaactggcgg agcagggaat tattttctgt tcctttggtg
    15181 aggcgatcca cgatcacccg gaactggtgc gtaaatatct cggcaccgtg gtgccgggga
    15241 atgacaactt ctttgccgcg cttaatgcgg cggtagcctc tgacggtacg tttatttatg
    15301 tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta ttttcgcatt aacgcggaaa
    15361 aaaccgggca gtttgagcgc accattctgg tggccgacga agacagctac gtcagctaca
    15421 ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt acacgcggca gtggtggaag
    15481 tcatcatcca taaaaacgcc gaggtgaaat attccacggt acaaaactgg ttccctggcg
    15541 ataacaacac cggcggtatt ctcaacttcg tcaccaagcg tgctttgtgc gaaggcgaaa
    15601 acagcaaaat gtcatggacg caatcagaaa ccgggtccgc gattacgtgg aaatatccca
    15661 gctgcatttt gcgcggcgat aactccattg gtgagtttta ctcagtggcg ctgaccagcg
    15721 gtcatcagca agcggatacc ggcaccaaga tgatccacat cggtaaaaac accaaatcga
    15781 ccattatctc gaaagggatc tctgccggac atagtcagaa cagttatcgc ggcttagtga
    15841 aaatcatgcc gacggcaacc aatgcgcgca atttcactca gtgcgactca atgctgattg
    15901 gcgctaattg tggggcgcat accttcccgt atgtcgaatg tcgcaataac agcgcacaac
    15961 tggagcacga ggcaacgaca tcacgtattg gtgaagatca actgttttac tgcctgcaac
    16021 gcgggatcag cgaagaagac gccatctcga tgattgttaa cggtttctgc aaagacgtgt
    16081 tctcagaact gccgctggaa tttgccgttg aagcacaaaa actcctcgcc atcagtcttg
    16141 aacacagcgt cggataagga ataaacatgt taagtattaa agatttacac gtcagcgtgg
    16201 aagataaagc tatcctgcgt ggattaagcc tcgccgttcg tcccggcgaa gttcacgcca
    16261 ttatggggcc aaacggttcg ggcaaaagta ccttatcggc aacgcttgcc gggcgcgaag
    16321 attatgaagt gacgggcggc acggttgagt tcaaaggcaa agatttgctt gcactgtcgc
    16381 cggaagatcg tgcaggcgaa ggcatcttta tggccttcca gtatccggtg gagattccag
    16441 gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc agtgcgcagc tatcgcggcc
    16501 aggaaacgct cgaccgcttt gattttcagg atttgatgga agagaaaatc gctctcctga
    16561 agatgccgga agatttatta acccgttcgg taaacgttgg tttttccggc ggcgagaaaa
    16621 agcgcaacga tattttgcaa atggcggtgc tggaaccgga gttatgcatt cttgatgagt
    16681 cggactccgg gctggatatt gacgcattaa aagtggtcgc cgatggcgtg aactcgctgc
    16741 gtgatggcaa gcgctcattc atcattgtta cgcactacca acgcattctc gactacatca
    16801 agcctgatta cgtccatgtg ctatatcggg gacgaattgt gaaatccggc gatttcacgt
    16861 tggtcaaaca actggaggag cagggttatg gctggcttac cgaacagcag taacgcgctg
    16921 caacagtggc atcacttgtt tgaagctgaa gggacaaaac gctcaccgca agcacagcag
    16981 catttacaac aattgctgcg taccggactg ccgacacgta aacatgaaaa ctggaaatat
    17041 acgccgctgg aagggctgac caatagccag tttgtcagca ttgcgggaga gatatcccca
    17101 cagcagcgag atgccttagc gttaacgtta gacgctgtgc ggctggtatt tgtcgatgga
    17161 cgttacgtgt cagcactgag cgatgcgact gaaggcagcg ggtatgaagt gagcattaac
    17221 gacgaccgtc agggggtacc cgacgctatt caggcggaag tgtttctgca tttgacggaa
    17281 agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg gtcaacggcc ggcaaagcca
    17341 ttgctgttaa tgcatattac ccagggcgtg gcaggtgaag aggtcaacac tgcccattac
    17401 cgacatcatc tggatctggc ggaaggtgcc gaagcaacgg tgatcgaaca ttttgtcagc
    17461 cttaatgatg ctcgtcactt caccggcgca cggttcacta tcaatgtcgc agcgaacgcc
    17521 cacttgcagc atatcaagct ggcgtttgaa aacccggtca gtcaccactt tgcccataac
    17581 gatttgttgc tggctgacga tgccaccgca tttagccata gtttcctgct gggtggcgca
    17641 gtgttacgac acaacaccag tacgcaactc aatggcgaaa acagcacgct gcggatcaat
    17701 agcctggcga tgccggtgaa aaacgaggtg tgtgataccc gtacctggct ggaacacaat
    17761 aaaggttttt gtaacagccg acagttgcac aaaactatcg tcagcgacaa aggccgcgcg
    17821 gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca aaacggatgg tcagatgacc
    17881 aacaataatc tgctgatggg caaactggca gaagtggata cgaaaccgca gctggaaatc
    17941 tatgcagatg atgtgaaatg cagccacggc gcgacggtgg ggcgtattga tgatgaacag
    18001 atgttctatc tgcgctcgcg cgggatcaat cagcaggatg cccagcagat gatcatttac
    18061 gcctttgctg ccgaactgac ggaagcactg cgtgatgagg ggcttaaaca gcaggtgctg
    18121 gcccgaatcg gtcaacggct gccaggaggt gcaagatgac tttttccgtc gacaaagtgc
    18181 gggccgactt tccggtgctt tctcgtgagg taaacggttt gccgctggct tatctcgaca
    18241 gcgccgccag tgcgcagaaa ccgagccagg tgattgacgc cgaggccgag ttttatcgtc
    18301 atggctacgc ggcggtgcat cgcggtattc ataccttaag cgcccaagcg accgagaaaa
    18361 tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc ccgttcggcg gaagagctgg
    18421 tgttcgtccg cggcacgacg gaagggatca atctggtcgc caatagctgg ggtaacagca
    18481 atgtgcgggc gggcgataac atcatcatca gtcagatgga gcaccacgct aacattgttc
    18541 cctggcagat gctttgcgca cgcgttggcg cagagctgcg tgtgatcccg ctcaatcccg
    18601 acggtacgtt gcaactggag acgctgccta cgctgtttga tgagaaaact cgcctgctgg
    18661 caattactca tgtctccaac gtgcttggca cagaaaatcc actggcggaa atgatcacgc
    18721 ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc tcaggcggtg atgcatcatc
    18781 cggtggatgt tcaggcgctg gattgcgact tttacgtgtt ctccgggcat aaactgtatg
    18841 gccccaccgg aattggcatt ctttatgtga aagaagcctt gttgcaggag atgccgccgt
    18901 gggaaggggg cggttctatg atcgccaccg tcagcctgag tgaaggcaca acctggacca
    18961 aagcaccttg gcggtttgaa gccggtacac ccaataccgg gggcatcatt ggtcttggcg
    19021 cggcgctgga gtatgtttcg gcgctggggc ttaataacat agccgagtat gaacagaatc
    19081 tgatgcacta cgcgctatca cagctggaat ctgtaccgga tctcactctc tatggtccgc
    19141 agaacaggct tggcgttatt gcttttaatc ttggtaaaca ccacgcctat gatgttggca
    19201 gttttctcga taactacggc attgctgtgc gtaccggaca tcactgcgct atgccattaa
    19261 tggcctatta caacgtccct gctatgtgtc gggcgtcgct ggccatgtat aacacccatg
    19321 aagaagtgga tcgtctggtg accggactgc aacgtattca ccgtctgctg ggataacagg
    19381 gaggcactat ggctttattg ccggataaag aaaaattgct gcgtaatttt ttacgctgcg
    19441 ccaattggga agagaaatat ctctacatta ttgagctggg ccagcgtctg ccagaattac
    19501 gcgacgaaga cagaagccca caaaatagca ttcagggctg ccagagtcag gtgtggattg
    19561 tcatgcgcca gaatgcgcag ggaattattg aattgcaggg cgacagcgat gcggcgattg
    19621 tgaaagggct tattgcggtc gtctttattc tctacgatca gatgacgccg caggatattg
    19681 tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct cacccaacat ctcaccccat
    19741 ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg cgccaaagcc gctgcactta
    19801 gctaaactaa agggacagct ttcatccggt tggctcacat tgccggatgg tcaatgttta
    19861 cactgacctg cacaggagtg tttcggtttc aggtgaacat aagcgccatc cgacgtttca
    19921 gcgtgagtct ccggcaaata caggaggttt acaatgaaac gcgcgtcttt gcttacactc
    19981 acgcttatcg gcgcttttag cgccatccag gctgcctggg cggttgatta tccgctacca
    20041 ccaaccggaa gccgactggt tgggcaaaat caaacgtata cggtgcagga aggggataaa
    20101 aaccttcagg ccatcgcccg acgttttgat actgcggcaa tgttgatcct tgaagccaat
    20161 aacactatcg ccccggtgcc aaaacccggt acgacgataa ctattccttc gcaactgtta
    20221 ttacctgatg cgccgcgtca ggggattatc gttaaccttg cagagctgcg cctttattat
    20281 tatccgccgg gagaaaatat cgtgcaggtt tatccgatag gtattggctt gcaggggctg
    20341 gaaacgccgg tgatggaaac gcgtgtgggg cagaaaatcc ctaacccaac ctggacgcct
    20401 actgcaggca ttcgtcaacg ttcgctggag cgtggtatta aattaccgcc agtcgttcct
    20461 gccggaccaa ataacccgct aggacgttac gcactgcgcc tcgcgcatgg taatggcgaa
    20521 tacctcattc atggcaccag tgcgccggac agcgtcggtt tgcgcgtcag ttcagggtgt
    20581 attcgcatga atgcaccgga tattaaagcc ttgttctcca gcgtgcggac gggaacgccg
    20641 gtgaaagtga tcaacgaacc tgtgaaatat tccgtggagc ctaacgggat gcgttatgtt
    20701 gaagtacatc gaccactatc ggcagaagaa cagcagaacg ttcagacaat gccatacaca
    20761 ctgccagcag gctttacgca atttaaagac aataaggctg tagatcagaa gttagtcgat
    20821 aaagcgttgt atcgtcgggc agggtatccg gtttcggtga gcagtggagc aactcccgca
    20881 gccagcaatg cgccttcagt agagtcagcg cagaatggtg aaccagagca agggaatatg
    20941 ttacgcgcga cgcagtagcg gtaaacggca gacaaaaaaa tggcgcacaa tgtgcgccat
    21001 ttttcacttc acaggtacta ttacttgcgg tatttagtag ccatgttgtc cagacgctgg
    21061 ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg aacgcattgc gttcacgtcg
    21121 ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt cagaagacag ctgatcgatt
    21181 ttagcgttgc tggagcaacc tgccagcaga gtagaaccca ggattaccgc gcccagtacc
    21241 agtttagtag ctttcattat taataccctc tagattgagt taatctccat gtagcgttac
    21301 aagtattaca caaagttttt tatgttgaga atattttttt gatgggaagg cacttatttt
    21361 tgatcgttcg ctcaaagaag catcgaacgc tgggttttag ataaaaaaat tgagagaaaa
    21421 caggatgctt ccatcggatt catcttagat aaagtgagat tatatagtga aatccgattt
    21481 gattttttat tgcttctggt tatcgattaa ataaaaaaag cgcccatcag ggcgcttcga
    21541 tatacaaatt aattcacaaa agcaatatta caggacgtga acagatgcgg tgttagtagt
    21601 gccgctcggt accagtgcac cagaaaccat aactacaacg tcacctttgt gtgccagacc
    21661 gctctgcaga gccagttctt tacccagacg gtagaaatca tcagtagaag tgatctcttt
    21721 aaccagctgc ggcacaacgc ctttgctcag taccaactga tgagccgttt tttcgttggt
    21781 ggtcagtgcc aggatggtgg catccgggaa gtatttacgt actgcgcgag cagatttacc
    21841 gccctgagta gcaaccacga tcagcggagc atccagtttt tcagcagttt caacggcacc
    21901 acggcatacc gcttcggtaa tgcgcagttt acggttgtca ttgttgaact cgagacggct
    21961 gttcatcacg cggtcggtac gttcgcaaat ggtcgccatg atagaaaccg cttccagcgg
    22021 gtatttacct tttgcggatt caccagacag catcactgca tcagtaccgt cgaggatggc
    22081 gtttgcaacg tcaccggctt ctgcgcgagt cgggcgtggg tttttgatca tggaatccag
    22141 catctgggtc gcagtgataa cgactttacg tgcacggata catttttcga tcatcatctt
    22201 ctgggcgaag ataacttctt ctaccgggat ttctacaccc aggtcgccac gcgcaaccat
    22261 gatgccgtca gaggcttcga ggatttcgtc gaagttgttg aggccttcct ggttttcgat
    22321 tttggagatg atgtggatgt tttcgccgcc gtgcgctttc aggtgctcac ggatttcgat
    22381 aacgtcagaa cgcttacgaa taaaggaagc agcaacaaag tctacgcctt gttcgcaacc
    22441 aaagatcagg tcctgtttgt ctttttcagc cagtgctggc agagcaatgg aaacgccagg
    22501 caggttcaca cctttgtttt cgcccaggtc accgttgttc agcactttac agataacttt
    22561 gttaccttca atggcggtaa cttccatacc gatcagacca tcgtcaacca gtacggtgtt
    22621 gccaactgac aggtcagtag tgaaaccttc atacgttacc gcaaccattt cgctgttgcc
    22681 gataacagat ttatcagtgg tgaaagtaaa ggtctgacca gctttcagag aaacgtcgtt
    22741 accgccttcc agtttcatgg tgcggatttc cggacctttg gtatcaagca ggatagcggc
    22801 ggttttacca gttttgctca tcacgttgcg cagattctga atgcgctgac cgtgttctgc
    22861 atagtcacca tgagagaagt tcagacgcat aacgttcatg ccagcgtcca gcattttagc
    22921 taacatctct tcagattcgg ttttcggtcc gatggtgcaa acaattttgg tctttttcat
    22981 gacagtctta gtctttaagt tgagaaggat gggagaaact tgctttctgg gcgcactgcc
    23041 ggaaagcgaa tccggtatta cgaaagtggt ggtgcctcgc tctaaggata ggtgacatcg
    23101 aaaaagcgtg cagaggaatg tgtgcttgtg tctcagccca acgtgagtgc ctgtttttga
    23161 gtcgttgagt tctacagtcc aaagtgctga aaccattcaa gagtcaattg gcgcgcatta
    23221 tacgctgaaa ttaatccaaa aggaaaacaa aaacagcgtt tcaaaaaata tacaacttgt
    23281 tagacaataa attgttatca aagatttaac gttccaggga aaaggttacg caaaaacttc
    23341 gctgtttgcg caacgtctgg tgccaaagtt gttagtgtga ggataagtga aagttcatcc
    23401 ggtgtataaa tatatcatcg ctcaattgct atagttaaaa taatttatcg cgttatttgt
    23461 ctgattttgt ttgatttttg tttaattggt tagattgata tatgctgata gttggcatga
    23521 taaataggta taaagaatgg gaaatcggac aaaagaggat gaactgtacc gagaaatgtg
    23581 cagagttgtc ggtaaagttg tgctggaaat gcgcgatctg gggcaggaac caaagcatat
    23641 tgttatcgca ggcgtattac gtacagcatt agccaataaa cgcattcagc gcagtgagct
    23701 ggaaaagcag gcgatggaaa ccgtcattaa cgcactggta aaatgaccat tcaggatgag
    23761 gcggtaagct tcatcctgat attcttgctt cagttgcggt ccagttaatc attctaaata
    23821 acaactgctg aa
//
LOCUS       DAZFWL020000066        23266 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFWL020000066 DAZFWL020000000
VERSION     DAZFWL020000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.066"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(480..1571)
                     /gene="ychF"
                     /locus_tag="ACHKNP_RS13885"
                     /old_locus_tag="pgaptmp_002777"
     CDS             complement(480..1571)
                     /gene="ychF"
                     /locus_tag="ACHKNP_RS13885"
                     /old_locus_tag="pgaptmp_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGU4704914.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1688..2272)
                     /gene="pth"
                     /locus_tag="ACHKNP_RS13890"
                     /old_locus_tag="pgaptmp_002778"
     CDS             complement(1688..2272)
                     /gene="pth"
                     /locus_tag="ACHKNP_RS13890"
                     /old_locus_tag="pgaptmp_002778"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGU4704915.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2550..2828
                     /gene="ychH"
                     /locus_tag="ACHKNP_RS13895"
                     /old_locus_tag="pgaptmp_002779"
     CDS             2550..2828
                     /gene="ychH"
                     /locus_tag="ACHKNP_RS13895"
                     /old_locus_tag="pgaptmp_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HGU4704916.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2883..4562)
                     /gene="dauA"
                     /locus_tag="ACHKNP_RS13900"
                     /old_locus_tag="pgaptmp_002780"
     CDS             complement(2883..4562)
                     /gene="dauA"
                     /locus_tag="ACHKNP_RS13900"
                     /old_locus_tag="pgaptmp_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HGU4704917.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4687..5634)
                     /gene="prs"
                     /locus_tag="ACHKNP_RS13905"
                     /old_locus_tag="pgaptmp_002781"
     CDS             complement(4687..5634)
                     /gene="prs"
                     /locus_tag="ACHKNP_RS13905"
                     /old_locus_tag="pgaptmp_002781"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGU4704918.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5785..6636)
                     /gene="ispE"
                     /locus_tag="ACHKNP_RS13910"
                     /old_locus_tag="pgaptmp_002782"
     CDS             complement(5785..6636)
                     /gene="ispE"
                     /locus_tag="ACHKNP_RS13910"
                     /old_locus_tag="pgaptmp_002782"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HGU4704919.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHASFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6636..7259)
                     /gene="lolB"
                     /locus_tag="ACHKNP_RS13915"
                     /old_locus_tag="pgaptmp_002783"
     CDS             complement(6636..7259)
                     /gene="lolB"
                     /locus_tag="ACHKNP_RS13915"
                     /old_locus_tag="pgaptmp_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HGU4704920.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7473..8729
                     /gene="hemA"
                     /locus_tag="ACHKNP_RS13920"
                     /old_locus_tag="pgaptmp_002784"
     CDS             7473..8729
                     /gene="hemA"
                     /locus_tag="ACHKNP_RS13920"
                     /old_locus_tag="pgaptmp_002784"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HGU4704921.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8771..9853
                     /gene="prfA"
                     /locus_tag="ACHKNP_RS13925"
                     /old_locus_tag="pgaptmp_002785"
     CDS             8771..9853
                     /gene="prfA"
                     /locus_tag="ACHKNP_RS13925"
                     /old_locus_tag="pgaptmp_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGU4704922.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9853..10686
                     /gene="prmC"
                     /locus_tag="ACHKNP_RS13930"
                     /old_locus_tag="pgaptmp_002786"
     CDS             9853..10686
                     /gene="prmC"
                     /locus_tag="ACHKNP_RS13930"
                     /old_locus_tag="pgaptmp_002786"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU4704923.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10683..11075
                     /gene="sirB2"
                     /locus_tag="ACHKNP_RS13935"
                     /old_locus_tag="pgaptmp_002787"
     CDS             10683..11075
                     /gene="sirB2"
                     /locus_tag="ACHKNP_RS13935"
                     /old_locus_tag="pgaptmp_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HGU4704924.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11079..11888
                     /gene="sirB1"
                     /locus_tag="ACHKNP_RS13940"
                     /old_locus_tag="pgaptmp_002788"
     CDS             11079..11888
                     /gene="sirB1"
                     /locus_tag="ACHKNP_RS13940"
                     /old_locus_tag="pgaptmp_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HGU4704925.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYVQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11924..12778
                     /gene="kdsA"
                     /locus_tag="ACHKNP_RS13945"
                     /old_locus_tag="pgaptmp_002789"
     CDS             11924..12778
                     /gene="kdsA"
                     /locus_tag="ACHKNP_RS13945"
                     /old_locus_tag="pgaptmp_002789"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HGU4704926.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12927..13034)
                     /locus_tag="ACHKNP_RS13950"
                     /old_locus_tag="pgaptmp_002790"
     CDS             complement(12927..13034)
                     /locus_tag="ACHKNP_RS13950"
                     /old_locus_tag="pgaptmp_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4704927.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13462..13569)
                     /locus_tag="ACHKNP_RS13955"
                     /old_locus_tag="pgaptmp_002791"
     CDS             complement(13462..13569)
                     /locus_tag="ACHKNP_RS13955"
                     /old_locus_tag="pgaptmp_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4704928.1"
                     /translation="MTLAQFAMIFWHDLAASILAGIITAAIVGWWRNRK"
     gene            complement(13998..14105)
                     /locus_tag="ACHKNP_RS13960"
                     /old_locus_tag="pgaptmp_002792"
     CDS             complement(13998..14105)
                     /locus_tag="ACHKNP_RS13960"
                     /old_locus_tag="pgaptmp_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4704929.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(14510..15610)
                     /gene="chaA"
                     /locus_tag="ACHKNP_RS13965"
                     /old_locus_tag="pgaptmp_002793"
     CDS             complement(14510..15610)
                     /gene="chaA"
                     /locus_tag="ACHKNP_RS13965"
                     /old_locus_tag="pgaptmp_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HGU4704930.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            15880..16110
                     /gene="chaB"
                     /locus_tag="ACHKNP_RS13970"
                     /old_locus_tag="pgaptmp_002794"
     CDS             15880..16110
                     /gene="chaB"
                     /locus_tag="ACHKNP_RS13970"
                     /old_locus_tag="pgaptmp_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HGU4704931.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            16268..16963
                     /gene="chaC"
                     /locus_tag="ACHKNP_RS13975"
                     /old_locus_tag="pgaptmp_002795"
     CDS             16268..16963
                     /gene="chaC"
                     /locus_tag="ACHKNP_RS13975"
                     /old_locus_tag="pgaptmp_002795"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HGU4704932.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(17007..17360)
                     /gene="ychN"
                     /locus_tag="ACHKNP_RS13980"
                     /old_locus_tag="pgaptmp_002796"
     CDS             complement(17007..17360)
                     /gene="ychN"
                     /locus_tag="ACHKNP_RS13980"
                     /old_locus_tag="pgaptmp_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HGU4704933.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            17545..18939
                     /gene="ychO"
                     /locus_tag="ACHKNP_RS13985"
                     /old_locus_tag="pgaptmp_002797"
                     /gene_synonym="iatA"
     CDS             17545..18939
                     /gene="ychO"
                     /locus_tag="ACHKNP_RS13985"
                     /old_locus_tag="pgaptmp_002797"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HGU4704934.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(18940..19590)
                     /gene="narL"
                     /locus_tag="ACHKNP_RS13990"
                     /old_locus_tag="pgaptmp_002798"
     CDS             complement(18940..19590)
                     /gene="narL"
                     /locus_tag="ACHKNP_RS13990"
                     /old_locus_tag="pgaptmp_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HGU4704935.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(19583..21379)
                     /gene="narX"
                     /locus_tag="ACHKNP_RS13995"
                     /old_locus_tag="pgaptmp_002799"
     CDS             complement(19583..21379)
                     /gene="narX"
                     /locus_tag="ACHKNP_RS13995"
                     /old_locus_tag="pgaptmp_002799"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HGU4704936.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            21718..23109
                     /gene="narK"
                     /locus_tag="ACHKNP_RS14000"
                     /old_locus_tag="pgaptmp_002800"
     CDS             21718..23109
                     /gene="narK"
                     /locus_tag="ACHKNP_RS14000"
                     /old_locus_tag="pgaptmp_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HGU4704937.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         5713 a         5819 c         5990 g         5744 t
ORIGIN      
        1 aaacaatatg cgccattttt gcattactaa taagaaaaag caaaaaacat cgatattcat
       61 ttttggtcaa tgggttttct ggcctatcgt tgttattgtg ctactggtta tcattttcat
      121 cttgccagga tcgcacgtaa tcacgttatt cggggacttt atctgcggga tattaccctt
      181 caggtaagtc cttttctcgc cagcatattg gagcgtgatc aatattgatc agctgtgaag
      241 ggggtgatgt gtagtggacc aaaacttacg ttatatcact actaatgaga tatccgtcat
      301 ctgacactga tgtctttaat tattctcgtg gttcactgta ggcaataagc acaaaagtgt
      361 aggatgttac aagaatgatt aggactcggt gaaatgaaaa atccacgcaa ttgcgtggat
      421 tatatatact tttgcgctct tcatgagatt tagcgaaacc tcatgagaca acaaattaat
      481 tagacgttga aaaggaagtt catcacatcg ccatctttaa cgatgtaatc tttaccttct
      541 gcacgcattt tgcctgcttc tttcgcgcct tgttcacctt tgtaagtgat gaaatcttca
      601 aacgagatgg tttgtgcacg gataaagcct ttttcaaaat cagtatggat tttgcccgct
      661 gcctgcggcg cggttgctcc aaccggaatg gtccatgcac gcacttcttt caccccagcg
      721 gtgaagtaag tttgcaggtt cagcagctta taaccggcac ggatcacacg gttcaggccc
      781 ggctcttcca gcccaagctc ctgcataaac tcgtcacgtt cttcgtcgtc cagttcggca
      841 atgtctgctt caacagcagc acaaaccgga accacaacag aaccttcttt cgccgcgatt
      901 tcacgcacct ggtcaagata tgggttgttt tcaaaaccgt cttcgttgac gttggcgatg
      961 tacattgttg gtttcagcgt caggaagctc aggtaacgaa tagccgcttt ctcttcagcg
     1021 cttaaatcca gcgcgcgcag catacctgca ttttccaact ggggcaggca tttttccagg
     1081 accgccagct cagctttcgc gtctttatcg ccacctttgg ctttcttctg tacgcgatga
     1141 atcgcacgtt cgcaggtgtc gaggtctgcc agcgccagtt cggtgttgat aacttcaata
     1201 tcgtcagccg ggttaacttt gccggaaacg tgaatgatgt tgtcattttc aaagcagcga
     1261 acaacgtgac cgatcgcttc ggtttcacgg atgttggtca ggaactggtt acccagacct
     1321 tcgcctttcg atgcgccttt taccagaccg gcgatatcga caaattccat ggtcgtggga
     1381 agcgtacgtt gcggttttac gatttcagcc agttgatcta ggcgagggtc aggcattggt
     1441 acgacgcctg tgttcggctc aatggtgcag aatggaaagt tggccgcttc aataccggct
     1501 ttggtcagcg cgttgaacag ggtagatttc ccgacgttgg gcaaaccgac gataccgcat
     1561 ttgaatccca tgatttaaat caccttaata tcttaataat caacctgtta ttgctaacag
     1621 attgcagaaa tggaaataac tttgcctatt atacacggca ctcggcaaaa atgccgcaga
     1681 caacgactta ttgcgcttta aaggcgtgca atcggttcgt tgctttggtc aagccatctg
     1741 taaaccacat ttcagtacaa cgcgccgctt cgtcgatggc ttcatcaatt aacttctgtt
     1801 cactaacagg cggtttgcct aacacaaaac cgacaacttt atttttatcg cccggatgac
     1861 cgattccgat gcgtaaacgg tgaaagttag ggttattacc caatttactg atgatgtctt
     1921 tcagtccatt gtgaccacca tggccaccgc ccaatttaaa tttggcgacg ccaggaggca
     1981 gatccagttc gtcgtgggcc accagaattt cgtccggatt aatgcggaaa aaactggcca
     2041 tcgccgcaac ggctttgccg ctgagattca taaatgtagt cgggactaac aggcggacat
     2101 cttcgcctcc aagagtgact cgcgaagtat aaccaaagaa tttagcctct tcgcgcagcg
     2161 gagcgcgcaa acgctctgcc agtaagtcaa cgaaccaggc accagcatta tgtcgcgttg
     2221 cggcgtattc agcaccgggg ttcgccaggc cgacaatcaa tttaatcgtc acgttttttt
     2281 gtcctgagtg tgtacataac tggcgcgtag tttactggtt gcggccccgc ttgacaaaaa
     2341 actgcgtatc aaatgcagat aacgtaataa ttgcatgagt ggactattag aaagtcaagg
     2401 tgttcaggcg tttatttgta aagttttgtt gaaataaggg ttgtaattgt gatcacgccc
     2461 gcacataacc cacagggtgt tgtctatact ttacacataa ggaagagggg tattccctgt
     2521 tacaacccag aaagttccgg aggtgacata tgaaacgcaa aaacgcttcg ttactcggta
     2581 acgtgctcat ggggttgggt ctggtggtaa tggtggtcgg cgtggggtat tcaatcctca
     2641 accagttacc acagtttaat atgccccagt atttcgcaca tggtgcagtg ctaagtattt
     2701 tcgtcggtgc cattctctgg ctggcgggtg cccgtgttgg cgggcatgaa caggtgtgcg
     2761 accgttactg gtgggttcgc cactatgaca aacgttgccg ccgtagcgat aatcgccgtc
     2821 atagctaaca aataatgcag tttgctgacc agtcagtttg cgctgactgg tcaatctcgt
     2881 acttataaat ccgccatcgc cgcgcgacga ttcgggaaga acgccaggcg tcccgggatc
     2941 ggttgaatgc cagcgcgagc catagtgcgc agtggctgga attccacgtt gcacacgcgc
     3001 agttcacatc cctcgggcag acgcttcaca aaacgctgga acgcatcaag accaccagca
     3061 tcaagtaccg gaaccgcatc ccacttcaga atcacaatcc gtttgccttc aagacgtgac
     3121 tccaggtccg tgaacaagcc ttcagcagca gcaaaaaaca gcgggccaat aacgcgcaga
     3181 accaggacat cgtctggaac atctacgact accggtgcca ggcgagtcat acgtgcgata
     3241 cgacgcataa acagcagcga tgccagcacg atccccacgc tgatggcaat aaccatatca
     3301 aacagcacgg tcagcgacat gcacagcagc atgacaatga tgtcatcttt cggcgcatga
     3361 cgcagcaagt cgaccacttt gtgcgcttca ctcatgttcc acgccaccat caacagcagg
     3421 gcagccatgg cggaaagcgg cagccaggag agcagcggtg ccagtaccag cagggcaaga
     3481 ataaccagaa tagagtggat caccgccgag ataggggaag ttgccccggc acggacgtta
     3541 gcggcagaac gcgcgatggc agctgtagcg gtaatgccac caaagaacgg agcgatgata
     3601 ttccccagtc cctgtccaac cagttcgcta ttcgccttgt gtttcgtccc ggtcatacca
     3661 tccagcacca cggcgcagag cagagattcg attgcgccga gcattgccat tgagaatgcc
     3721 gcaggcagca gtgtgcgaat agaatcccag gttagcgtga attctgaatt aggcagatcc
     3781 cacggcagca ccagttgcgg cagcagttgc ggaataccgt taccctgaga accatcggcc
     3841 aggacgtagt ggaattgcga accgatggta gcaacatgtc cgccgagcag gttaacaatc
     3901 cccatcaccg cgcaaccagc cagcaaggcc ggaaggtgac cgggtaaacg aatgcccaga
     3961 cgcggccaga aaacaagaat acctagcgtc acaatgccaa tggcagcatc acccacatta
     4021 atggtcggca gcgccataaa taatgcgccg actttttgta gataatgttc cgggacatgg
     4081 gccatttgca gaccgagaaa atctttaatc tgcatggtac cgatggtgat cccgataccc
     4141 gaggtgaaac ctaaggtgac ggaaaccgga atatactcaa tcaggcgacc aaagcgtgcc
     4201 agacccatca gaatcaaaaa aatccccgac agcaaggtcg caaccagcag tcctgccagt
     4261 ccaaactgtt gcgacacggg atagagaatt accacaaatg ccgcagtcgg accggaaacg
     4321 ctaaagcgtg acccacccgt cagagcaatg acaatccccg caacagctgc ggtatataaa
     4381 ccgtactggg gtgccacacc actaccaata gccaacgcca tcgccagcgg gatagcaata
     4441 atcccgacgg ttatcccggc aatcaggtca cgggtaaacc gtgcggcagt atatttttct
     4501 ttccagcaag cgtcgatcag agcgcggaaa ggcatcacat gtgaggaaaa tattttgttc
     4561 acaataatgt ttcatccgtg agcgcatcat ctgtcaacta aatggcaggt gaaggaggca
     4621 taggtcatac aaatggatat tacagacaaa aaaacccgcc gcagcgggtc tttgagccgg
     4681 gttcgattag tgttcgaaca tggcagagat cgattcttcg ttgctgatac gacgaatcgc
     4741 ttcggccagc atacctgaca gggtcagagt acgcacgttc ggcagtgatt tgatttcatc
     4801 gctcagcgga atggtatcgc agacaacgac ttcatcaatt acagagttac gcaggttatt
     4861 cgccgcgttg ccagagaaga tcgggtgagt cgcgtacgca aatacacgtt tagcaccacg
     4921 ttctttcaga gcttcagcag ctttacacag cgtaccgcca gtgtcgatca tatcatcgac
     4981 cagtacgcag tcacgacctg caacgtcacc gatgatatgc atcacctggg aaacgttcgc
     5041 acgcggacga cgtttgtcga tgattgccat atcggtatcg ttcagcagct tagcgatagc
     5101 gcgggcacgc acaacgccgc cgatgtccgg agaaaccaca attgggttat ccagattcag
     5161 ctgcagcatg tcttccagca ggatcgggct accaaatacg ttatcaaccg gaacgtcgaa
     5221 gaaaccctga atctgttcag cgtgcagatc cactgtcagc acacggtcaa caccgacgct
     5281 ggagaggaag tctgcaacca ctttcgcagt gattggtaca cgagcggaac ggacgcgacg
     5341 gtcctggcgc gcatagccaa agtaggggat aacagcggtg atacgacctg cggaagcacg
     5401 acgcagggca tcaaccataa cgactaattc catcaggttg tcgttagtag gggcacaagt
     5461 ggactggatg atgaaaatat caccaccgcg tacattttcg ttgatttgta cactgacttc
     5521 gccgtcgcta aagcgaccta cagcggcgtc gccgagtgaa gtgtacaggc ggttggcaat
     5581 acgttgtgct agttccgggg tggcgttacc agcaaaaagc ttcatatcag gcacgagaag
     5641 aacctcaggc atgcgtccat tggtggaaag aatctgccga aaactgtgcg ggccaggcat
     5701 gatcctttcc aggcggtgta ttaaagagcg cgatgcaacg tctggaacaa ggtgacgttg
     5761 tcaccgaaac tcagcttgcc cggcttaaag catggctctg tgcaatgggg aaagattaac
     5821 gcctttcgcc acaaagccat tgagccattc cggggcttgc tctagcacct ggcgggcttc
     5881 agactctgta tcaaattcag caaagacaca ggcccctgtc ccagtcaggc gcgacggggc
     5941 gtattctaac agccaggaaa gcaccgcatc aacctcgcga aaacgttttc ttgcgataac
     6001 ctcgcaatca ttgctgaatt cacattttag caacgtttct attgaccttt ttggcgtatt
     6061 gcgcgggagt tcaggatctt taaaaatcac cggagtcgga atacttacac cagggtgcgc
     6121 caccagatac cacttctctg gcggatccac cggcgttagt atttcaccaa cgccttcggc
     6181 aaacgaggca tgcccccgaa caaagacagg aacatctgcg cccagcgtca gccccatttc
     6241 cgccagctca tccatgctta gcccgcattg ccagagatga tttaatgcca ccaggaccgt
     6301 cgcggcattg gatgaaccac cgccgagacc gccgcccatc ggcaaacgct tgtcaatgct
     6361 gatattcgca ccgcttcccg tcggaagacg cccgctgtct gccgcagttt tcatcaacaa
     6421 tcgcgctgcg cgaacgatca ggttatcttc atgttccacg ccttcaacgg gcgttaacag
     6481 acgaatatcc ccatcgtcac gaagctcaat gctgatggtg tcgccgtaat caagaaactg
     6541 aaacagcgtt tgcagcgtgt ggtaaccatc cgcacgctga ccggtaatgt ataaaaacag
     6601 attaagtttt gccggagagg gccactgtgt ccgcattatt tcactatcca gttatccatt
     6661 tttaacttga tgcgttgacc accgtcggtg agttccatat tggctggcat cgcaggttgc
     6721 gttttggtgt cataaccacc ataaacaacc ttccagtttt tgccattctg gctgtaggta
     6781 atttcgctca ggcggtactg gtcgtccagt ttgtagtcgg ttgcatcacc cggtaaacct
     6841 aaaatccact ggcgcaagct gttgagcgga attggcattc cggtcaattt gccaatcatc
     6901 tcttcggcgt catcggcggt ataacgctga cctttattgt cgactaactg cacgttaccc
     6961 ggttgagcat tcagctccag ttccgtgctg cccaatgggt tagtgagcag cagacggtag
     7021 cgatcctggc cggtttgctg ccagaaaaag cgggcgtaca ctttttgttg gtcagaaata
     7081 taagcgaacg cgccgcgagt ctgatactga ttaagattgc gcacgtcttg ctgatgctga
     7141 cgccattgtg gcgaatccgg gcttttgcca ggacctttgg gcgtggtaac ggaacaggca
     7201 gtgagcacaa gagcagccag cggtagcagg cggataagac gaaaatcggg caggggcata
     7261 gtgatgacaa gtccttgaga tacgttgcag ttataaccct taatgctagc gttaccgtcc
     7321 gctatcgtct atgttcaagt tgtcttaatt gccagaatct aacggctttc ggcaattact
     7381 ccaaaagggg gcgctctctt ttattgatct tacgtatcct gtatgatgca agcagactaa
     7441 ccctatcaac gttggtatta tttcccgcag acatgaccct tttagcactc ggtatcaacc
     7501 ataaaacggc acctgtatcg ctgcgagaac gtgtatcgtt ttcgccggat aagctcgatc
     7561 aggcgcttga cagcctgctt gcgcagccga tggtgcaggg cggcgtggtg ctgtcgacgt
     7621 gcaaccgcac ggaactttat cttagcgttg aagagcagga taacctgcaa gaggcgttaa
     7681 tccgctggct ttgcgattat cacaatctta atgaagaaga tctgcgtaaa agcctctact
     7741 ggcatcagga taacgacgcg gttagccatt taatgcgtgt tgccagcggc ctagattcac
     7801 tggttcttgg ggagccgcag atcctcggtc aggttaaaaa agcgtttgcc gattcgcaaa
     7861 aaggtcatat gaaggccagc gaactggaac gcatgttcca gaaatctttc tctgtagcga
     7921 aacgcgttcg cactgaaaca gatatcggtg ccagtgctgt gtctgtcgct tttgcggctt
     7981 gtacgctggc gcggcagatc tttgaatcgc tctctacggt cacagtgttg ctggtaggcg
     8041 cgggcgaaac catcgagctg gtagcgcgtc atctgcgcga acataaagta cagaagatga
     8101 ttatcgccaa ccgcactcgc gaacgtgccc aaattctggc agatgaagtt ggcgcggaag
     8161 tgattgccct gagtgagatc gacgaacgtc tgcgcgaagc cgatatcatc atcagttcca
     8221 ccgccagccc gttaccgatt atcgggaaag gcatggtgga gcgcgcatta aaaagccgtc
     8281 gcaaccaacc aatgctgttg gtggatattg ccgttccgcg cgatgttgag ccggaagttg
     8341 gcaaactggc gaatgcttat ctttatagtg tggacgatct gcaaagcatc atttcgcaca
     8401 acctggcgca gcgtaaagcc gcagcggttg aggcggaaac tattgtcgct caggaaacca
     8461 gcgaatttat ggcgtggctg cgagcacaaa gcgccagcga aaccattcgc gagtatcgca
     8521 gccaggcaga gcacgttcgc gatgagttaa ccgccaaagc gttagcggcc cttgagcagg
     8581 gcggcgacgc gcaagccatt atgcaggatc tggcatggaa actgactaac cgcttgatcc
     8641 atgcgccaac gaaatcactt caacaggccg cccgtgacgg ggataacgaa cgcctgaata
     8701 ttctgcgcga cagcctcggg ctggagtagc agtacatcat tttctttttt tacagggtgc
     8761 atttacgcct atgaagcctt ctatcgttgc caaactggaa gccctgcatg aacgccatga
     8821 agaagttcag gcgttgctgg gtgacgcgca aactatcgcc gaccaggaac gttttcgcgc
     8881 attatcacgc gaatatgcgc agttaagtga tgtttcgcgc tgttttaccg actggcaaca
     8941 ggttcaggaa gatatcgaaa ccgcacagat gatgctcgat gatcctgaaa tgcgtgagat
     9001 ggcgcaggat gaactgcgcg aagctaaaga aaaaagcgag caactggaac agcaattaca
     9061 ggttctgtta ctgccaaaag atcctgatga cgaacgtaac gccttcctcg aagtccgagc
     9121 cggaaccggc ggcgacgaag cggcgctgtt cgcaggcgat ctgttccgta tgtacagccg
     9181 ttacgccgaa gcccgccgct ggcgggtaga aatcatgagc gccagcgagg gtgaacatgg
     9241 tggttataaa gagatcatcg ccaaaattag cggtgatggt gtgtatggtc gtctgaaatt
     9301 tgaatctggc ggtcatcgcg tgcagcgtgt tcctgctacg gaatcgcagg gtcgtattca
     9361 tacttctgct tgtaccgttg cggtaatgcc agaactgcct gacgcagaac tgccggacat
     9421 caacccagca gatttacgca ttgatacttt ccgctcgtca ggggcgggtg gtcagcacgt
     9481 taacaccacc gattcggcaa ttcgtattac tcacttgccg accgggattg ttgttgaatg
     9541 tcaggatgaa cgttcacaac ataaaaacaa agcgaaggcg ctttcagtgc tcggtgctcg
     9601 catccacgct gctgaaatgg caaaacgcca acaggccgaa gcgtctaccc gtcgtaacct
     9661 gctggggagt ggcgatcgca gcgaccgtaa ccgtacttac aatttcccgc aggggcgcgt
     9721 taccgatcac cgcatcaacc tgacgctcta ccgcctggat gaagtgatgg aaggtaagct
     9781 ggatatgctg attgaaccga ttatccagga acatcaggcc gaccaactgg cggcgttgtc
     9841 cgagcaggaa taatggaata tcaacactgg ttacgtgaag caataagcca acttcaggcg
     9901 agcgaaagcc cgcggcgtga tgctgaaatc ctgctggagc atgttaccgg cagagggcgt
     9961 acttttattc tcgcctttgg tgaaacgcag ctgactgacg aacaatgtca gcaacttgat
    10021 gcgctactga cacgtcgtcg cgatggtgaa cccattgctc atttaaccgg ggtgcgagaa
    10081 ttctggtcgt tgccgttatt tgtttcgcca gcgaccttaa ttccgcgccc ggatacggag
    10141 tgtctggtgg agcaggcact ggcgcggttg cctgaacaac cttgccgtat tcttgatctc
    10201 gggacgggta ccggggcgat tgcgcttgcg ctggctagcg agcgcccgga ctgcgaaatt
    10261 atcgctgtag atcgtatgcc tgatgctgtc tccctggcac aacgtaatgc ccagcatctg
    10321 gcgatcaaaa atatccacat tctgcaaagc gactggttta gcgcgctagc cgggcagcag
    10381 tttgcgatga ttgtcagcaa tccgccgtat attgacgagc aggacccaca tcttcaacaa
    10441 ggcgatgtcc gctttgagcc gctcactgcg ctggttgcgg cagacagtgg aatggcagac
    10501 atcgtgcata tcatcgaaca gtcgcgtaac gcgctggtat ccggcggctt tctgcttctg
    10561 gaacatggct ggcagcaggg cgaagcggtg cgacaagcat ttatcctcgc ggggtatcat
    10621 gacgtcgaaa cctgccgtga ctatggtgat aacgagcgcg taacgctcgg ccgctattat
    10681 caatgacaag tttttctaca ctgcttagtg ttcatcttat tagtatcgcg ctttctgttg
    10741 ggctattaac cttacgtttc tggctacgtt atcagaagca tccacaggca tttgctcgct
    10801 ggacgcgcat tgtgccgccg gttgtcgata cggtgttatt gttaagcggc attgcgttga
    10861 tggctaaagc gcacatcctg ccattttccg ggcaggcaca gtggctgact gaaaagctgt
    10921 ttggagttat catttatatc gttttgggtt ttattgcact cgattatcgt cgtatgcaca
    10981 gtcagcaggc gcgcattatt gccttcccgc tggcgttggt ggtgctgtac atcatcatta
    11041 aactcgccac cacaaaagta ccgttactgg ggtaagtcat gagatcgtta gctgatttcg
    11101 aatttaataa agcgccattg tgcgaaggca tgatcctggc ttgcgaagca atccgccgcg
    11161 attttccctc gcaagatgtt tacgacgaac tggagcgtct cgttagtctg gcgaaggaag
    11221 aaatcagcca gcttctgcct ttagaagagc agctggaaaa actgatcgcg ctgttttacg
    11281 gcgactgggg atttaaagcc tcacgcggcg tttatcgtct ttccgatgca ttatggctgg
    11341 accaggtgtt aaagaatcga cagggcagtg cggtatcatt aggtgcggtt ttattatggg
    11401 tcgcgaatcg tctcgatttg ccgctgctgc cggtgatttt ccctacgcag ctgatattgc
    11461 gcattgaatg tccggatggc gaaatttggc tgattaatcc ttttaacggt gaatcgttaa
    11521 gcgaacatat gctggacgta tggttaaagg gaaatatcag cccgtcggcg gaactgtttt
    11581 atgaagacct tgatgaagct gataacattg aggtaatccg caaattgctg gatacactca
    11641 aagcctcgtt gatggaagaa aatcagatgg agctggcgtt acgcaccagc gaagctttat
    11701 tacaattcaa ccctgaagat ccctatgaaa ttcgcgatcg cgggttgatt tatgtgcaac
    11761 tggattgcga acacgttgcg ttgaacgatt taagttattt cgttgaacag tgtccggaag
    11821 acccgatcag cgaaatgatc cgtgcgcaaa taaataacat cgcgcataaa catattgtgc
    11881 tgcattaatt aatcgacatt ttactcaaga ttaaggcgat cctatgaaac aaaaagtggt
    11941 tagcattggc gacatcaacg tagcaaatga cctgccgttc gtactgtttg gcggtatgaa
    12001 cgtgctggaa tctcgcgatc tggcgatgcg catttgcgag cactacgtaa ctgtgaccca
    12061 gaaactgggt atcccttacg tgttcaaagc ctcttttgac aaagccaacc gctcctccat
    12121 ccactcttat cgtggaccgg gcctggaaga agggatgaaa atcttccagg agttgaagca
    12181 gacttttggc gtgaaaatta tcaccgacgt tcacgaacca agtcaggcac agcccgttgc
    12241 tgatgtcgtg gatgtgattc agttgccggc gtttcttgct cgccagactg acctggttga
    12301 agccatggcg aaaaccggtg cggtaattaa cgtcaagaaa ccacagtttg tcagcccggg
    12361 acagatgggt aatatcgttg ataaattcaa agaaggcggc aacgaaaaag tgattctttg
    12421 cgatcgtggt gctaacttcg gctatgacaa cctggttgtc gatatgctgg gcttcagcat
    12481 tatgaagaaa gtgtctggta actcgccggt gattttcgac gtgacccacg cactgcaatg
    12541 ccgcgatccg tttggcgcag cttccggtgg tcgtcgtgct caggtggctg agctggcacg
    12601 agccggtatg gcggtaggtc tggcggggct gtttattgaa gcgcatccgg atccggaaca
    12661 tgcgaaatgt gatggtccat ccgcgctgcc gctggctaaa ctggaaccgt tcctcaagca
    12721 gatgaaagcg attgatgatc tggtgaaagg tttcgaagaa ctggatacca gcaagtaatc
    12781 ttttttgctt gaaaaataaa gtcttagcgt tctgcgttaa gacttttttc atgggtgcca
    12841 gatacaaaaa aggccgcagg ctgttacccc tgcggccggt ttcgggcgca tattgccatc
    12901 acggcagcct gacgcccgtt ttcaccttac ttccggttac gccaccagcc gacaatcgct
    12961 gcggtaataa ttcccgccag gatcggtgct gccaggtcgt gccagaaaat catggcaaac
    13021 tgcgcgagcg tcatatagcc gccttgttgt aatgacaaca ttttgcggct attcttgaat
    13081 tgttctggtt caagattagc ccccgttctg ttgtcaggtt aaacctctca acgtgcgggg
    13141 gttttctctt tccagcaacc aatgccacca gggataaagc ccccgcaaca ttgcgcctca
    13201 ccggataacg ccggcttgat gtggatacta cgtctcaatt catcttcact tcatccctga
    13261 aatgtttgca atgaagagtg cattccggtt tttcaacagc tgttacagtc gtttcatgag
    13321 ggctctggat gatgcttcca gctcgggttg ccaatgttta ctggcggaag agataaagac
    13381 aaaaaaggcc gcaggctgtt acccctgcgg ccggtttcgg gcgcatattg ccatcacggc
    13441 agcctgacgc ccgttttcgc cttacttccg gttacgccac cagccgacaa tcgctgcggt
    13501 aataattccc gccaggatcg atgctgccag gtcgtgccag aaaatcatgg caaactgcgc
    13561 gagcgtcata tagccgcctt gttgtaatga caacattttg cggctattct tgaattgttg
    13621 atggttcaag attagccccc gttctgttgt caggttttac ctctcaacgt gcgggggttt
    13681 tctctttcca gcaaccaatg ccaccaggga taaagccccc gcaacattgc gcctcaccgg
    13741 ataacgccgg cttggtgtgg atactacgac tcaattcatc ttcacttcat ccctgaaatg
    13801 tttgcaatga agagtgcatt ccggtttttc aacagctgtt acagtcgttt catgagtgct
    13861 ctggatgatg cttccagctc gggttgccaa tatttacttg tggaagagat aaagacaaaa
    13921 atggccgcag gctgttaccc ctgcggccgg tttcgggcgc atattgccat cacggcagcc
    13981 tgacgcccgt tttcacctta cttccggtta cgccaccagc tgacaatcgc tgcggtaata
    14041 attcccgcca ggatcggtgc tgccaggtcg tgccagaaaa tcatggcaaa ctgcgcgagc
    14101 gtcatatagc cgccttgttg taatgacaac attttgcggc tattcttgaa gtgtctggtt
    14161 tcaagattag cccccgtttt gttgtcaggt tttacctctc aacgtgcggg ggttttctct
    14221 ttccagcaac caatgccacc agggataaag cccccgcaac attgcgcctc accggataac
    14281 gccggcttgg tgtggatact acgtcgcaat tcatcttcac ttcatccctg aaatgtttgc
    14341 aataaagagt acattccggc ttttcaacag ctgttgcagt ggtttcgtgc aggttgtggt
    14401 acaggctcgc aattctgatt aacgagctgt ccaggtagta tgaagaaata caataagcag
    14461 gaatttatct tatctccccc gatttccgtc atcggaggag atataactgt caggcaaata
    14521 tcgtcatcaa ataggcggca aacagtgcca gatgcgctgc gccattgagc acgttagtac
    14581 gtccggtgga gaaggagata tggcacagca ctaaagaggc caccatcacc accatttctg
    14641 gcgcaccaag tgcaaactgt aattcgttac ccgtcataaa ggcaattagc gtgacgacag
    14701 gtacggtaag cgaaatggtt gctaacactg aaccaaagaa cagattcatc gcgcgctgga
    14761 cctggttgtt caacactgct tttaacgcac ctaaaccttc cggcgacaga atcaacagag
    14821 ccaccaggaa gccagtaaag gcgacagggg cattcatgct gtcgagcaat gtctccagcg
    14881 ggctggcatt cattttggtg actgcaataa cggcaatcag atggataatc aaccagatag
    14941 catgccacac gctgctatgg gcagacggtt taccgtgatg cgggtcatcg tcatcactgt
    15001 catcttcgtg ctcgtagaca aacaaacttt gatgcgtttt ggtctggatc agcaaaaata
    15061 cgccatacat tgccgcagaa attaatgcta ccagtaacgc ctgaccggtt gaaaaattcg
    15121 ccgcaggcag agccattgga aataccagta cgattatcgc cagggggaac agcgcaatta
    15181 aatactgctt gataccaaac agattcatat attgggtggc aaacttacga ccgcccaaca
    15241 ataatgaaaa gccaaccagc ccaccggtaa caatcataat gattgaatag agcgtatcac
    15301 gcattagcgt tggcgcggcg tcgccggttg ccattaaagc tgaaatcaaa ctgacttcaa
    15361 gaataaccac tgaaaggcta agaataagcg aaccgtaagg ttctcccagg cgatgggcta
    15421 atacgtccgc atgacggaca acactaaagg cgctacttaa aataccaata agcgcaagaa
    15481 gattgatggc aatgaccact ggtagtgtct ggctgcttcc ccacaggaac agcactacca
    15541 gcgccagaac cgggaaaata agcgaagtct ccttgtggcg ggtttttacc gcctcttgag
    15601 catttgacat tatggttatc cctttgcaga tgaatttatc gaaaatgtaa aaaataggta
    15661 ggaaaaataa cagaaagtgt ctggatatcg gtaacatttt acgaattttt accccactgt
    15721 cgatttttta ctcattggga cataaaaata agtattacgt ttagacaatg tttgttttag
    15781 cgtttcttaa gaacagtctg acatgaaaat tcttatgttt tggcaagagt agatattgtt
    15841 gaccacactt aatgttcaac tttgtaaaag gagtcaacga tgccgtataa aacgaaaagc
    15901 gatctgccgg aaagcgtaaa gcacgttcta ccgtctcatg cccaggatat ctataaagaa
    15961 gcgttcaaca gcgcatggga tcaatataaa gataaagacg atcggcgtga tgacgccagt
    16021 cgcgaagaaa ccgcgcataa agtggcctgg gctgctgtga agcatgaata tgccaaaggg
    16081 gatgatgata aatggcataa aaaatcgtaa aaccggtagc ttagttaaag ctattcgtgc
    16141 ggtgttgcct tgcaagtggt ccgtggattg catattgtcc cgttagtggc ttcaaaatga
    16201 gcagtaaaaa tgtccggaag acaccaaaaa gttgtcgcag ggaagtatgc agtggcggag
    16261 gtgtaaggtg ataacgcgtg atttcttgat gaatgccgat tgtaaaacgg catttggtgc
    16321 cattgaagaa tcactcttat ggtcagcaga gcaacgggcg gcttcgctgg cggcgacgct
    16381 ggcttgtcga cctgatgagg gaccggtgtg gatcttcggt tatggatcgt tgatgtggaa
    16441 tccggcactg gagtttaccg agtcgtgcac cggtacactg gttggatggc atcgcgcgtt
    16501 ttgcctgcgc ctgaccgccg ggcgcgggac tgcgcaccag ccgggacgga tgcttgcact
    16561 gaaagagggc ggacgcacta caggcgtcgc ctatcgactg ccagaagaga cgctggagca
    16621 ggaactaacc ctgttgtgga agcgcgagat gattaccggc tgttatctgc caacctggtg
    16681 tcagcttgat cttgatgatg gatgcacagt aaacgccatt gtgtttatta tggacccgcg
    16741 acatccagaa tatgaatctg acactcgcgc tcaggtcatc gcgccgttga ttgcggcggc
    16801 gagcggtccg ctgggaacca acgcacaata cctgttttca ctggaacagg agcttatcaa
    16861 actgggaatg caggacgatg ggctgaatga tttgctggta tcggtaaaaa aactgctggc
    16921 ggagaattat ccggatggtg tgttacggcc gggattcgcc tgagtaaact tccctcatag
    16981 tggggcgtca gacgccccct caaacattaa aatgtgagca ctttatcggc tgacagcgtc
    17041 cattgcgcca gttccacaag agtaccgatt tccaccccat caatcagagg aagtgtacta
    17101 atcccgcgcc cgtcggtaca ggttttgcac aatttcaccg gtacattctg agcggtaagg
    17161 atctccagca tttgctgaat gttgtagcct tcccctggtt tttgcccgcg caacccggct
    17221 gtgaccgcat cagacatcag gaacagacgc agatccagat tgctctcctg ctctcgtaac
    17281 gcaatggcta gccgcaagct gttaaacaag gattcgctcc cgtaaggtgc gccattggca
    17341 acgatcacga ttttttgcat tatttactcc tgtattcagg gaattagaca ttcatcttct
    17401 atcttactgc ttctgcagcg tctgaccaat cggtcacatt tttaaggatt ttcctgaaag
    17461 cgcgagaaaa tacgacaaaa gttgccagta atcgttattc tttaaggcta tggtttttca
    17521 ttattaccgg aagttaccga cgttttgagc cgtttcgttc ctcgcattat cccgttttat
    17581 ttactcttgc ttgtagcagg cggtacagct aacgcacaat ctaccttcga gcaaaaagcg
    17641 gcaaatccct ttgataataa caatgatggt ctgccggatt taggcatggc tcccgaaaat
    17701 catgatgggg aaaaacactt tgctgaaatt gtgaaagatt tcggcgaaac cagtatgaat
    17761 gataacgggc tggatactgg cgagcaggca aaagctttcg cattgggaaa agtccgcgac
    17821 gcgcttagtc aacaggttaa tcagcacgta gagtcctggc tatcaccgtg gggaaatgcc
    17881 agtgttgacg tcaaagtgga taacgaagga catttcaccg gcagtcgtgg aagctggttt
    17941 gtgccgttac aagataatga tcgttatctc acctggagcc agcttggtct tactcagcag
    18001 gatgatgggt tggtgagcaa tgtgggcgtt gggcaacgct gggcgcgcgg caactggctg
    18061 gtgggttata acacttttta tgacaacttg ctggacgaaa atcttcagcg agcgggcttt
    18121 ggtgccgaag cgtggggcga atatttgcga ctatcggcaa acttttatca gccgtttgct
    18181 gcatggcatg aacagacagc cacgcaggaa caacggatgg cgcgcgggta tgacctgaca
    18241 gcccggatgc gcatgccgtt ctatcaacac ctcaatacca gtgtcagcgt agaacagtat
    18301 tttggtgatc gtgttgattt gtttaactct ggtacgggtt atcacaatcc cgtcgcgttg
    18361 agtctgggat taaattacac ccctgtgcca ttagtcactg tgacggccca gcataaacag
    18421 ggtgaaagtg gcgagaatca aaataacctc gggctgaatc ttaattaccg ctttggtgta
    18481 ccgctcaaaa aacaactttc tgcgggtgag gttgccgaaa gtcagtcgtt acgtggtagt
    18541 cgctatgaca atccgcagcg aaataatcta ccgactcttg agtaccgaca gcgaaaaacg
    18601 ttaacggtgt ttctggcgac accgccgtgg gatctaaaac ctggcgaaac agtgccgctg
    18661 aaattacaaa tccgcagtcg ttacggtatt cggcaactga tttggcaggg cgatacgcag
    18721 atattaagtt tgacgccggg cgcacaagcc aacagcgcgg agggctggac gctgatcatg
    18781 cctgactggc agaacgggga aggggcaagc aatcactggc gattgtcggt ggtggtggaa
    18841 gataaccagg ggcagcgtgt ctcctccaat gagatcacgc taacgcttgt cgaaccgttc
    18901 gatgcattgt caaacgacga actgcgctgg gaaccgtaat cagaaaatgc gctcctgatg
    18961 cacccatacc gctgcttcca cgcgagactt gagcttcatt ttcttcagca tgtgcttgac
    19021 gtgcactttt actgtgcttt cggtgatatc caggcggcgg gcaatcatct tgttcggcaa
    19081 accctgggca atcagcttga gaatatcgcg ctcgcgtggg gttaactggt taacatcgcg
    19141 ctcagtagtg gcacggttag cgcgcaagct ggcggccaga acaggcgtta atgcttcgct
    19201 taataccatt tcgccagcag ctgcctgatg caatgctttc agcagatctt ccggttccat
    19261 atcttttaac agataaccat ccgcgccgcg tttcagtgcg gtgaccacat cttcttcatg
    19321 gttagagacg ctgaatacca caatgcgccc tgagagggac ttttcgcgca gtttatccag
    19381 cgtttccaga ccgttcatgc cgggcatatt gagatctaac aggatcagat cgggatcaag
    19441 agactccgcc agttcaatac cctgttcgcc attactcgct tcgccaacca cggtgatatc
    19501 tggtgccata ctgataagct gttttacgcc agttcgcagc atcgggtgat cgtcaatcag
    19561 caggatagta gccggttcct gattactcat gggtatctcc ttggacgtct gtgaaagttt
    19621 tttcgggaat aaaggtgacc accacttcgg tgccacctga ttcacgacgg cggacgcggc
    19681 aatcgcctcg taaactttgc gcacggtcgc gcattattat catgccgtag tgattgctgc
    19741 ggatggcatt ttcaggcacg ccgcagccgt tatcctggac ggtcagtttg acctgattat
    19801 cgttttgcgc caccgtcacc acgacttcac tcgcttgcga gtgtttgagg gcgttactta
    19861 atgcctcacg ggcaatttgc aacaagtgga ttgcctgatg cgaaggcacc aggcgaggcg
    19921 gcaattgata atccagcttc accgggaagc caaatttggc gctgtactct tcgcaactcg
    19981 cctccagcgc cggacgtaat ccaggctcgg tgagctgcaa gcggaatgtg gtgagcaatt
    20041 cacgcaactg cgcccaggat gcattcagtt cgttacggat ctgacttaac agttcgcggc
    20101 tgctttctgg cagcgcatcg ccctgcatct gtaaacaact cacctgcatc ttcatgcaag
    20161 agagagattg ggcaatagaa tcatgcagtt cgcgcgcaat ggtggcacgc tcttccatca
    20221 cgatcaactg ttgctgacgt tcctgatggc gatccagcgc cagcgtggcg gtgagttgtt
    20281 caaccagggt atccaccagt tgttgttgat catggctaag atgacgcccc tgcggcaggg
    20341 tcgccagcaa aataccgtac tgcgtatgag agtcagccag ccgccacttc agggttgtgc
    20401 cgcgatcacc aacgggtaat acgccgcgcg ggcagagctg gcagccttta tcatcacaag
    20461 tcatatctgg ttggcaggta aactcctgat gattctcttc atcatcagta tcataaaccc
    20521 gcagttcgat atcacgtagc agggttaaat tctgtaagcc gttgagtaca ggtgacaggc
    20581 gttcacacag cggggcttgg gaatgcaaac ggcggttagc ctgccataaa aaagagagga
    20641 tctgattttt atgctccagc ccggcggttt tctcctgaac ccgctgctca agtacggcat
    20701 aactttcggc cagttctgca gacatattgt tcaacgcagt tccaagcatc gccatttcgt
    20761 tgcgcccgct gatgtttgcg cgttgggtaa aatcgcgatg actgacggca ctcgccattg
    20821 ccagcagttg ccgccacggt tgtagcagtc gcgcccgcaa ccagataata gtgaacacca
    20881 gtaaaagtgc cataaatacc gccattaccc gatggaccag taccactgtc tcgatgcgca
    20941 tttccgtggt gcggtcaaaa ccagatacca gttgatcaag cccggcaaca aactggctga
    21001 catccgctga caccgtttct cggttttgtg cacgcatcaa cgcagggatc agttcattac
    21061 gccagtaatc ttgtaaaccc tgtaattgcg ccagttgtcc gtctcgttct gctgctcgag
    21121 tcaactcggc gctaaatgcc gtttgttcca tctctttaat taagggcttg tctttctcgc
    21181 ttaatggcac tgctgccaac agacggtaac tttgcatgcg cagcgatccc gctttgttga
    21241 tcgcatgggc gctgccctga acgccttgca ccagccagcc agaaaccgcc atccctgcca
    21301 gtccaatagc agtagaaagc aacacaataa gcgcaacctg attaaccagg gtgagcggag
    21361 agagacaacg tttaagcatg taaacctctt ccttcaggct ttaaatgagc aataacctta
    21421 atgaatgtga cgatacattc tggaatggca gtattctcgg ctattggctg aagtataccc
    21481 atacctggaa agagttactc cttatttgcc gtgtggttag tcgctttaca tcgttaaggg
    21541 tagggattct acagcaccgt gaaaaatctc ataattttta tgaaatcact gtactcacta
    21601 tgggtaatga taaatatcaa tgatagataa agttatctta tcgtttgatt tacatcaaat
    21661 tgcctttagc tacagacact aaggtggcag acatcgaaac gagtatcaga ggtgtctatg
    21721 agtcactcat ccgcccccga aagggctact ggagctgtca ttacagattg gcgaccggaa
    21781 gatcctgcgt tctggcaaca acgcggtcaa cgtattgcca gccgcaacct gtggatttcc
    21841 gttccctgtc tgctgctggc gttttgcgta tggatgttgt tcagcgctgt tgcggtgaac
    21901 ctaccgaaag tcggctttaa ttttacgacc gatcagctat ttatgttgac tgcgctgcct
    21961 tcggtttctg gcgcgttatt acgtgttcca tactccttta tggttcctat cttcggtggt
    22021 cgtcgctgga cggcgttcag caccggtatt ctgattattc cttgcgtctg gctgggtttt
    22081 gccgtgcagg atacctccac gccttatagc gtcttcatca tcatctctct gctatgcggc
    22141 tttgctggcg cgaacttcgc ctccagtatg gcaaacatca gcttcttctt tccgaaacag
    22201 aagcagggtg gcgcgctggg tctgaatggt ggtctgggca acatgggcgt cagcgtcatg
    22261 cagttggttg ctccgctggt ggtatcactg tcgattttcg cagtatttgg tagccagggc
    22321 gtcaaacagc cggatgggac tgagctgtat ctggcgaatg cgtcctggat atgggtgccg
    22381 ttccttgcca tcttcactat tgcggcgtgg tttggcatga acgatcttgc tacctcgaaa
    22441 gcctccatca aggagcagtt gccggtactc aaacggggtc atctgtggat tatgagcctg
    22501 ctgtatctgg caaccttcgg ctccttcatc ggcttctccg cgggctttgc aatgctgtca
    22561 aaaacgcagt tcccggatgt tcagattctg caatacgctt tcttcgggcc gtttattggt
    22621 gcgctggcgc gttctgcagg tggtgcatta tctgaccgtc tgggcggaac tcgtgtcacg
    22681 ctggtgaact ttattctgat ggcgattttc agcggcctgc tgttcctgac cttaccgact
    22741 gacgggcagg gcggaagctt catggcgttc ttcgcggtct tcctggcgct gttcctgaca
    22801 gctgggctgg gtagtggttc cactttccag atgatttcag tgatcttccg taaactgaca
    22861 atggatcgcg tgaaagcaga agggggttct gacgaacgtg cgatgcgtga agcggcaacc
    22921 gacacggcag cggcgctggg tttcatctct gcgattggcg ctattggtgg cttctttatc
    22981 ccgaaagcgt ttggtagctc gctggcatta acgggttcgc cagtcggcgc aatgaaggta
    23041 tttttgattt tctatatcgc ctgcgtagtg attacctggg cggtatatgg tcggcattct
    23101 aaaaaataaa ccgttactcg tcatacttcg ggttacatgt gctgcggctg cgttcattca
    23161 ccccagtcac ttactttagt aagctcctgg gattcattca cttgccgcct tcctgtaaac
    23221 cgaattatat agagtaaaat atttgattat cctttgcgcg gcatga
//
LOCUS       DAZFWL020000067        23213 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFWL020000067 DAZFWL020000000
VERSION     DAZFWL020000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.067"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            114..1478
                     /locus_tag="ACHKNP_RS14005"
                     /old_locus_tag="pgaptmp_002801"
     CDS             114..1478
                     /locus_tag="ACHKNP_RS14005"
                     /old_locus_tag="pgaptmp_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4704938.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(1606..2097)
                     /gene="yajQ"
                     /locus_tag="ACHKNP_RS14010"
                     /old_locus_tag="pgaptmp_002802"
     CDS             complement(1606..2097)
                     /gene="yajQ"
                     /locus_tag="ACHKNP_RS14010"
                     /old_locus_tag="pgaptmp_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HGU4704939.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2265..3176
                     /gene="panE"
                     /locus_tag="ACHKNP_RS14015"
                     /old_locus_tag="pgaptmp_002803"
     CDS             2265..3176
                     /gene="panE"
                     /locus_tag="ACHKNP_RS14015"
                     /old_locus_tag="pgaptmp_002803"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HGU4704940.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3139..3729
                     /gene="yajL"
                     /locus_tag="ACHKNP_RS14020"
                     /old_locus_tag="pgaptmp_002804"
     CDS             3139..3729
                     /gene="yajL"
                     /locus_tag="ACHKNP_RS14020"
                     /old_locus_tag="pgaptmp_002804"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HGU4704941.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3783..5231)
                     /gene="thiI"
                     /locus_tag="ACHKNP_RS14025"
                     /old_locus_tag="pgaptmp_002805"
     CDS             complement(3783..5231)
                     /gene="thiI"
                     /locus_tag="ACHKNP_RS14025"
                     /old_locus_tag="pgaptmp_002805"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGU4704942.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5437..5679
                     /gene="xseB"
                     /locus_tag="ACHKNP_RS14030"
                     /old_locus_tag="pgaptmp_002806"
     CDS             5437..5679
                     /gene="xseB"
                     /locus_tag="ACHKNP_RS14030"
                     /old_locus_tag="pgaptmp_002806"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGU4704943.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5679..6578
                     /gene="ispA"
                     /locus_tag="ACHKNP_RS14035"
                     /old_locus_tag="pgaptmp_002807"
     CDS             5679..6578
                     /gene="ispA"
                     /locus_tag="ACHKNP_RS14035"
                     /old_locus_tag="pgaptmp_002807"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HGU4704944.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6603..8465
                     /gene="dxs"
                     /locus_tag="ACHKNP_RS14040"
                     /old_locus_tag="pgaptmp_002808"
     CDS             6603..8465
                     /gene="dxs"
                     /locus_tag="ACHKNP_RS14040"
                     /old_locus_tag="pgaptmp_002808"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HGU4704945.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8645..9619
                     /gene="yajO"
                     /locus_tag="ACHKNP_RS14045"
                     /old_locus_tag="pgaptmp_002809"
     CDS             8645..9619
                     /gene="yajO"
                     /locus_tag="ACHKNP_RS14045"
                     /old_locus_tag="pgaptmp_002809"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HGU4704946.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFVSMQDHYNLIYREEESEMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9673..10191)
                     /gene="pgpA"
                     /locus_tag="ACHKNP_RS14050"
                     /old_locus_tag="pgaptmp_002810"
     CDS             complement(9673..10191)
                     /gene="pgpA"
                     /locus_tag="ACHKNP_RS14050"
                     /old_locus_tag="pgaptmp_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HGU4704947.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10169..11146)
                     /gene="thiL"
                     /locus_tag="ACHKNP_RS14055"
                     /old_locus_tag="pgaptmp_002811"
     CDS             complement(10169..11146)
                     /gene="thiL"
                     /locus_tag="ACHKNP_RS14055"
                     /old_locus_tag="pgaptmp_002811"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HGU4704948.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11224..11643)
                     /gene="nusB"
                     /locus_tag="ACHKNP_RS14060"
                     /old_locus_tag="pgaptmp_002812"
     CDS             complement(11224..11643)
                     /gene="nusB"
                     /locus_tag="ACHKNP_RS14060"
                     /old_locus_tag="pgaptmp_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGU4704949.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11663..12133)
                     /gene="ribE"
                     /locus_tag="ACHKNP_RS14065"
                     /old_locus_tag="pgaptmp_002813"
     CDS             complement(11663..12133)
                     /gene="ribE"
                     /locus_tag="ACHKNP_RS14065"
                     /old_locus_tag="pgaptmp_002813"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGU4704950.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12222..13325)
                     /gene="ribD"
                     /locus_tag="ACHKNP_RS14070"
                     /old_locus_tag="pgaptmp_002814"
     CDS             complement(12222..13325)
                     /gene="ribD"
                     /locus_tag="ACHKNP_RS14070"
                     /old_locus_tag="pgaptmp_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGU4704951.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13329..13778)
                     /gene="nrdR"
                     /locus_tag="ACHKNP_RS14075"
                     /old_locus_tag="pgaptmp_002815"
     CDS             complement(13329..13778)
                     /gene="nrdR"
                     /locus_tag="ACHKNP_RS14075"
                     /old_locus_tag="pgaptmp_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGU4704952.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13929..14468
                     /locus_tag="ACHKNP_RS14080"
                     /old_locus_tag="pgaptmp_002816"
     CDS             13929..14468
                     /locus_tag="ACHKNP_RS14080"
                     /old_locus_tag="pgaptmp_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU4704953.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14767..15651
                     /gene="tsx"
                     /locus_tag="ACHKNP_RS14085"
                     /old_locus_tag="pgaptmp_002817"
     CDS             14767..15651
                     /gene="tsx"
                     /locus_tag="ACHKNP_RS14085"
                     /old_locus_tag="pgaptmp_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HGU4704954.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15828..16175)
                     /gene="yajD"
                     /locus_tag="ACHKNP_RS14090"
                     /old_locus_tag="pgaptmp_002818"
     CDS             complement(15828..16175)
                     /gene="yajD"
                     /locus_tag="ACHKNP_RS14090"
                     /old_locus_tag="pgaptmp_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HGU4704955.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16304..17275)
                     /gene="secF"
                     /locus_tag="ACHKNP_RS14095"
                     /old_locus_tag="pgaptmp_002819"
     CDS             complement(16304..17275)
                     /gene="secF"
                     /locus_tag="ACHKNP_RS14095"
                     /old_locus_tag="pgaptmp_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HGU4704956.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17286..19133)
                     /gene="secD"
                     /locus_tag="ACHKNP_RS14100"
                     /old_locus_tag="pgaptmp_002820"
     CDS             complement(17286..19133)
                     /gene="secD"
                     /locus_tag="ACHKNP_RS14100"
                     /old_locus_tag="pgaptmp_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HGU4704957.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19161..19493)
                     /gene="yajC"
                     /locus_tag="ACHKNP_RS14105"
                     /old_locus_tag="pgaptmp_002821"
     CDS             complement(19161..19493)
                     /gene="yajC"
                     /locus_tag="ACHKNP_RS14105"
                     /old_locus_tag="pgaptmp_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGU4704958.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19516..20643)
                     /gene="tgt"
                     /locus_tag="ACHKNP_RS14110"
                     /old_locus_tag="pgaptmp_002822"
     CDS             complement(19516..20643)
                     /gene="tgt"
                     /locus_tag="ACHKNP_RS14110"
                     /old_locus_tag="pgaptmp_002822"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGU4704959.1"
                     /translation="MKFELDTTDGRARRGRLVFDCGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20699..21769)
                     /gene="queA"
                     /locus_tag="ACHKNP_RS14115"
                     /old_locus_tag="pgaptmp_002823"
     CDS             complement(20699..21769)
                     /gene="queA"
                     /locus_tag="ACHKNP_RS14115"
                     /old_locus_tag="pgaptmp_002823"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGU4704960.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21862..22443
                     /gene="acpH"
                     /locus_tag="ACHKNP_RS14120"
                     /old_locus_tag="pgaptmp_002824"
     CDS             21862..22443
                     /gene="acpH"
                     /locus_tag="ACHKNP_RS14120"
                     /old_locus_tag="pgaptmp_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HGU4704961.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22448..>23213)
                     /locus_tag="ACHKNP_RS14125"
                     /old_locus_tag="pgaptmp_002825"
     CDS             complement(22448..>23213)
                     /locus_tag="ACHKNP_RS14125"
                     /old_locus_tag="pgaptmp_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HGU4704962.1"
                     /translation="MQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAMNYR
                     GFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARF
                     KTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQDTAL
                     FALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEVVLP
                     ASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
BASE COUNT         5623 a         6212 c         5909 g         5469 t
ORIGIN      
        1 aaacatacct gacacacaaa ttactctcct ctgtaatatt ttctcattaa cactctgttt
       61 acccaacatg cgctccgcac tacactatgt gggcattttt ttaaggggtg gtaatgaacg
      121 attataaaat gacgccaggt gagaggcgcg cgacctgggg tttagggacc gtattctcgt
      181 tgcgcatgct gggcatgttt atggttctgc cggttctgac cacgtacggc atggctctgc
      241 aaggtgccag cgaagcatta atcggtattg ccattggtat ttatggtctg actcaggccg
      301 tttttcagat tccgtttggc ctgctttccg accgcattgg tcgcaaacca ttaattgtcg
      361 gtgggctggc ggtgtttgcc gccggtagcg ttatcgctgc gctttccgac tccatctggg
      421 gaattattct gggccgggcg ctacaaggct ctggagcaat tgccgccgcc gttatggcgc
      481 tgctttccga tctcacgcgc gaacaaaacc gcaccaaagc gatggcgttt atcggcgtga
      541 gctttggcat tacctttgcc attgcgatgg tgcttggccc gatcatcact cacaaacttg
      601 ggctgcacgc gctgttctgg atgatcgcta ttctggcaac aaccggcatt gcgttgacca
      661 tttgggttgt acccaacagt agcactcacg tacttaatcg tgagtccgga atggtgaaag
      721 gcagtttcag taaagtgctg gcggaaccgc ggctgctaaa actcaacttt ggcattatgt
      781 gtctgcacat gcttctgatg tctacgtttg ttgccctgcc cggacagcta gctgatgcgg
      841 ggttcccggc ggctgaacac tggaaggtct atctggcgac gatgttaatc gcctttggct
      901 cggtcgtgcc tttcattatc tacgctgaag ttaagcgcaa aatgaagcaa gtctttgtct
      961 tctgcgtcgg gttgatcgtg gttgcggaaa ttgtgttgtg gaacgcccaa acgcagttct
     1021 ggcaactggt ggtcggcgtg cagcttttct ttgtggcgtt taatttgatg gaagcccttc
     1081 tgccctcact tatcagtaaa gagtcgccag caggttacaa aggtacggcg atgggtgtct
     1141 actccaccag ccagtttctt ggcgtggcga ttggcggttc gctgggcggc tggattgacg
     1201 gcatgtttga cggtcagggg gtatttctcg ctggcgcaat gctggccgca gtgtggctgg
     1261 cagtcgccag taccatgaaa gaaccgccgt atgtcagcag tttgcgcatt gaaatcccgg
     1321 cggacattgc cgcaaacgaa gcgttaaaag tgcgtttgct ggaaactgaa ggcgtcaaag
     1381 aagtgttgat tgcagaagaa gaacattcag cttatgtgaa aatcgacagc aaagtgacga
     1441 atcgctttga gatagaacag gcaattcgtc aggcataaaa aacgcggcga attgcctgct
     1501 gcgctacgct tatcaggcct acaaagtttc tgaattacaa acctttgtag gccgaatcag
     1561 gcgttcatgc cgcatctgac atgaacaaaa cgcacatagt cgcgattaat cgcggaagtt
     1621 tttgaactgg aacggctgac cgagatcgcc accacgtacc atcgccatga cagcctgcaa
     1681 atcatcacga gatttgcccg ttacgcggat ttcatcgccc tgaatttgcg cctgcacttt
     1741 cagtttgctg tctttgatca tcttgacgat tttcttctgg gtcgcgctct caatgccctg
     1801 tttcagtttc gcttccacaa accaggtttt accgctatga acgatattct ccggtacatc
     1861 cagcgaactg ccttcaatgc cgcgcttcag cagcttggca cgcagaatat ccagcaactg
     1921 attgacctgg aaatcggact cgctcaacac tttgatggtt ttgctggcgt cgttcagctc
     1981 aaatgaggct tcaacgttac ggaagtcaaa acgggactcc acttcgcggc tcgcgttatc
     2041 gaccgcgtta cgtgcttcct gaagatcaac ttcagagaca atatcgaaag atggcatctt
     2101 ttcctctccc ttcatttttg atgcgaagca taatacccgc aaagttaaat aaccgacagt
     2161 cctgcaccag gacgtatccc ttaattgtgc atagtcgcaa cagcatgcac aattaaggga
     2221 tagccttatt gaacacccgg agtggttgcg ggtgaggagg aacaatgaaa attaccgtat
     2281 tgggatgcgg tgccttaggg caattatggc ttacagcact ttgcaaacag ggtcatgaag
     2341 ttcagggctg gctgcgcgta ccgcaacctt attgtagcgt gaatctggtt gagacagatg
     2401 gttcgatatt taacgaatcg ctgaccgcca acgatcccga ttttcttgcc accagcgatc
     2461 tgctcctggt gacgctgaaa gcatggcagg tttacgatgc cgtcaaaagc ctcgcgtcca
     2521 cactgcctgt aaccacgcca atactgttaa ttcacaacgg catgggcacc atcgaagagt
     2581 tgcaaaacat tcagcagcca ttactgatgg gcaccaccac ccatgccgcc cgccgcgacg
     2641 gcaatgtcat tattcatgtg gcaaacggaa tcacgcatat tggtccggca cggcaacagg
     2701 atggcgatta cagttatctg gcggatattt tgcaaaccgt gttgcctgac gttgcctggc
     2761 ataacaatat tcgcgccgag ctgtggcgca agctggccgt caactgtgtg attaatccac
     2821 tgaccgccat ctggaattgc ccgaatggtg aattacgtca tcatccgcaa gaaattatgc
     2881 agatatgcga agaagtcgcg gcagtgatcg agcgcgaagg gcatcatact tcagcagaag
     2941 atttgcgtga ttacgtgatg caggtgattg atgccacagc ggaaaatatc tcgtcgatgt
     3001 tgcaggatat ccgcgcgctg cgccacaccg aaatcgacta tatcaatggt tttctcttac
     3061 gccgcgcccg cgcgcatggg attgccgtac cggaaaacac ccgcctgttt gaaatggtaa
     3121 aaagaaagga gagtgaatat gagcgcatcg gcactggttt gcctcgcccc tggtagtgaa
     3181 gagactgaag ccgtcaccac tatcgatctg ctggttcgcg gcggtatcaa agtcaccact
     3241 gccagcgtcg ccagcgatgg caacctggcc attacctgct cgcgcggcgt gaagctgctg
     3301 gcggatgcgc cgctggtcga ggtggctgat ggcgaatatg acgtgatcgt actgcctggt
     3361 ggcattaaag gcgcagagtg ttttcgcgat agtaccctgc tggttgaaac cgttaaacag
     3421 ttccaccgtt ccgggcgtat cgtcgcggct atttgcgccg cgccagccac cgtgctggtg
     3481 ccgcacgata tcttcccgat tggtaatatg accggcttcc cgacgctgaa agacaaaatt
     3541 cccgctgaac aatggcagga caagcgcgtc gtctgggatg cacgggtaaa attgctgacc
     3601 agccaggggc cggggacagc tatcgacttt ggtctgaaaa ttatcgacct gctggttggg
     3661 cgcgagaaag cccatgaagt ggcatcacaa ctggtgatgg cggcagggat ttataattat
     3721 tacgagtagt gtcggatgcg gtaagcgtcg catctgacca gatgcgacgt tacaaccaca
     3781 aattacgggc gatatacctt cacattgtta aagccctgct cgcgcagata gagcgcctgc
     3841 agacggctca tcaccccgcg ctcacaccac agtagccagg ttctgttctg gtcgagatcg
     3901 ccaaacttgg tgctcagttt atagaacggc agagaaacta catcaatccc ttcgactttc
     3961 agtggcttat cttcctgttc atcgatagaa cggatatcga ggatcacgtc gttcgggccg
     4021 aagccattga cggtttccac ttccaccact tcctgctcgg tctgctgggc gatttcgcgg
     4081 atatcaacgt tattcgcttc ttcaaccact ttatcgagaa tgctgaagtc gaatttctct
     4141 tcttccgctt caatcttcga cttaaccgct ttcaccgtcg ggcttttgga gataacaccg
     4201 caatattccg gcatcgtgcg agcaaagtct tcggtgccaa tctggcgagc caggttgatg
     4261 atgtgctctt tgtcgtaaga aatcagcgga cgcaggatca gcgtatcgga gacgttatca
     4321 atcaggcgca ggttggtcag cgtctgactg gacacctggc cgagcgcttc gccggtgacc
     4381 agcgcctgta cgccgtaacg ttcagccact ttagacgcgg cacgcaccat catacgtttg
     4441 aggataacgc ccatctgacc gtcgtcgatt ttctcgagaa tttccccgac gaccggttcg
     4501 aaattaatag cgacaaaacg cacgcggtgg gagctgccaa aacggttcca cagataatgc
     4561 gccacctgac gcacgccaat ttcatgcgcc gcgccgccaa ggttaaagaa acagtaatgc
     4621 acgcggcagc cgcgacgcat caacatataa ctggaaacac cggagtcgaa accaccggaa
     4681 atgagcgaca gcacatcttc ctgggtgccg atcgggaaac cgccaatacc ttcgtagcgg
     4741 cctttaatca gcaggagacg atcgtcttcc acttccagat ggacagtcac ttccggattg
     4801 gtcagcttca cgcgcgcgga ctcaatatgc tgatttaaac cgccgccgac gtaacgttcc
     4861 acatcaatcg agctaaaatc atgtttgcca cggcgcttca cgcgtacgca gaaggttttg
     4921 ccttccagct gatcgcgata ctgaaccaac gctttctcaa aaatatcgtg catgtcggta
     4981 aacggcacgt cttcgacttc gagaatatgg tggatacccg gaatacgggt cagggcgtcg
     5041 cgaatagcca gacgctggtt ttcatctttt gcgcgaactt cgatgttatc ccagtggcgg
     5101 acgacagcga gcgtctcatc atagtgcttt aaaacgttac gaatgttccc ggtaaggatt
     5161 tttataaagc gcaagcgcac agattggctt ttgatggtga tttccgggaa caatttaatg
     5221 ataaacttca tggcggcaat ggttcgttgg caagccttaa gcgacttgta tagggaaaaa
     5281 tacagcagcc cacacctgcg gctgcatcca ggcgcggaag tataccacta acatcgcttt
     5341 gctgcgcaca tcaccttacc attgcgcgtt atttgctatt tgccctgagt ccgttaccat
     5401 gacggggcga aaaatattga gagtcagaca ttcattatgc cgaagaaaaa tgaggctccc
     5461 gccagctttg aaaaggcgct gagcgagctg gaacagattg taacccgtct ggaaagtggc
     5521 gacctgccgc tggaagaggc gctgaacgag ttcgaacgcg gcgtgcagct ggcacgtcag
     5581 gggcaggcca aattacaaca agccgaacag cgcgtacaaa ttctgctgtc tgacaatgaa
     5641 gacgcctctc taaccccttt tacaccggac aatgagtaat ggactttccg cagcaactcg
     5701 aagcctgcgt taagcaggcc aaccaggcgc tgagccgttt tatcgcccca ctgccctttc
     5761 agaacactcc cgtggtcgaa accatgcagt atggcgcatt attaggtggt aagcgtctgc
     5821 gacccttcct ggtttatgcc accggtcata tgtttggcgt tagcacaaac acgctggacg
     5881 cacccgctgc cgccgtagag tgtatccacg cttactcatt aattcatgat gatttaccgg
     5941 cgatggatga tgacgatctg cgtcgcggtt tgccgacctg ccatgtgaag tttggcgaag
     6001 caaacgcgat tctcgctggc gacgctttac aaacgctggc gttctcgatt ctaagcgatg
     6061 ccaatatgcc ggaagtgtcg gatcgcgaca gaatttcgat gatttctgaa ctggcgagcg
     6121 ccagcggtat tgccggaatg tgcggtggtc aggcattaga tttagacgcg gaaggcaaac
     6181 acgtacctct ggacgcgctt gagcgtattc atcgtcataa aaccggcgca ttgattcgcg
     6241 ccgccgttcg ccttggtgca ttaagcgccg gagataaagg gcgtcgtgct ctgccagtac
     6301 tcgacaagta cgcagagagc atcggccttg ccttccaggt tcaggatgac atcctggatg
     6361 tggtaggaga tactgcaacg ttgggaaaac gccagggtgc cgaccagcaa cttggtaaaa
     6421 gtacctaccc tgcacttctg ggtcttgagc aagcccggaa gaaagcccgg gatctgatcg
     6481 acgatgcccg tcagtcgctg aaacaactgg ctgaacaatc actcgatacc tcggcactgg
     6541 aagcgctagc ggactacatc atccagcgta ataaataaac aataagtatt aataggcccc
     6601 tgatgagttt tgatattgcc aaatacccga ccctggcact ggtcgactcc acccaggagt
     6661 tacgactgtt gccgaaagag agtttaccga aactctgcga cgaactgcgc cgctatttac
     6721 tcgacagcgt gagccgttcc agcgggcact tcgcctccgg gctgggcacg gtcgaactga
     6781 ccgtggcgct gcactatgtc tacaacaccc cgtttgacca attgatttgg gatgtggggc
     6841 atcaggctta tccgcataaa attttgaccg gacgccgcga caaaatcggc accatccgtc
     6901 agaaaggcgg cctgcacccg ttcccgtggc gcggcgaaag cgaatatgac gtattaagcg
     6961 tcgggcattc atcaacctcc atcagtgccg gaattggtat tgcggttgct gccgagaaag
     7021 aaggcaaaaa tcgccgtacc gtctgtgtaa tcggcgatgg cgcgattacc gcaggcatgg
     7081 cgtttgaagc gatgaatcac gcgggcgata tccgtcctga tatgctggtg gtcctcaacg
     7141 acaacgaaat gtcgatttcc gaaaatgtcg gcgcgctcaa caaccatctg gcgcagctgc
     7201 tttccggtaa gctttactct tcactgcgcg aaggcgggaa aaaagttttc tctggcgttc
     7261 cgccaattaa agagctgctc aaacgtaccg aagaacatat taaaggcatg gtagtgcctg
     7321 gcacgttgtt tgaagagctg ggctttaact acatcggccc ggttgacggt cacgatgtgc
     7381 tggggcttat caccacgctg aagaacatgc gcgacctgaa aggcccgcag ttcctgcata
     7441 tcatgaccaa aaaaggtcgt ggttatgaac cggcagaaaa agaccccatc actttccacg
     7501 ccgtgcctaa atttgatccc tccagcggtt gtttgccgaa aagtagcggc ggtttgccga
     7561 gctattcaaa aatctttggc gactggttgt gcgaaacggc agcgaaagac aacaagctga
     7621 tggcgattac tccggcgatg cgtgaaggtt ccggcatggt cgagttttca cgtaaattcc
     7681 cggatcgtta cttcgacgtg gcaatcgccg agcaacacgc ggtgaccttt gccgccggtc
     7741 tggcgattgg tgggtacaaa cccattgtcg cgatttactc cactttcctg caacgcgcct
     7801 atgatcaggt gctgcatgac gtggcgattc aaaagcttcc ggtcctgttc gccatcgacc
     7861 gcgcgggcat tgttggtgct gacggtcaaa cccatcaggg cgcttttgac ctctcttacc
     7921 tgcgctgcat accggaaatg gtcattatga ccccgagcga tgaaaacgaa tgtcgccaga
     7981 tgctctatac cggctatcac tataacgacg gcccgtccgc ggtgcgctac ccgcgcggta
     8041 acgcggttgg cgtggaactg acgccgctgg aaaaactgcc aattggcaaa ggcattgtga
     8101 agcgtcgtgg cgagaaactg gcgatcctta actttggtac gctgatgcca gacgcggcga
     8161 aagtcgctga atcgctgaac gctacgctgg tcgatatgcg ttttgtgaaa ccgcttgatg
     8221 aagcgttaat tctggaaatg gccgccagcc atgaagcgct ggtcaccgta gaagaaaacg
     8281 ccattatggg cggcgcaggc agcggcgtga acgaagtgct aatggcccat cgtaaaccag
     8341 tacccgtgct gaacattggc ctgcctgact tctttattcc acaaggaact caggaagaaa
     8401 tgcgcgccga actcggcctc gatgccgccg gtatggaagc caaaatcaag gcctggctgg
     8461 cataatccct actccactcc tgctatgctt aagaaattat tcatagactc taaataattc
     8521 gagttgcagg aaggcggcaa acgagtgaag ccccaggagc ttacataagt aagtgactgg
     8581 ggtgaacgaa tgcagccgca gcacatgcaa cttgaagtat gacgagtata gcgggagtgg
     8641 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8701 ctgtatgacc tttggcgagc cagatcgcgg taatcacgca tggacactgc cggaagagag
     8761 cagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8821 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8881 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8941 attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgccgtc tcggcatgga
     9001 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     9061 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     9121 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9181 tgtcagtatg caggatcact acaatctgat ttatcgcgaa gaagagagcg agatgctgcc
     9241 actgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9301 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9361 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacag gcgtcagcga
     9421 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttgttgagta aaccgggcat
     9481 tgccgcaccg attatcggaa cttcgcgcga agaacagctt gatgagctat tgaacgcggt
     9541 ggatatcact ttgaagccgg aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9601 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9661 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9721 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9781 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9841 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9901 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9961 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
    10021 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
    10081 aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact
    10141 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10201 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10261 tttgcccgat acaggtaaac ggtacgccca ggtgtccgag agccacatcc agcgcgccac
    10321 ggttcagttc cggcacagtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10381 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca
    10441 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10501 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10561 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10621 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10681 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10741 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10801 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10861 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10921 gatcgcttag gttcactgcc agtgctttat acgccagatc agcaggatcg atatcatgga
    10981 ggaagtggtt gcccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    11041 tattgagaag agcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    11101 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    11161 caggtaaaaa agaaaaggcc ggcgatcgcg gaacggtctt ccgtgaatct accggcctgg
    11221 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11281 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11341 tttgtatggc acatcgctac gtttagacag ctcgtacagc gcaatgcgca gtactgcttt
    11401 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11461 gtatgcggtg ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11521 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11581 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11641 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11701 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11761 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11821 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11881 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11941 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    12001 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    12061 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    12121 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12181 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12241 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12301 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12361 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12421 ggccccgctt ccacccagat actgttaatt tgctgtttac ccagttgcat catcagtaca
    12481 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12541 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12601 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12661 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12721 ggatcatccg ccagcaccgt ggcgctgcta gttaaaatgg catgactttg tgcgcgctgt
    12781 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12841 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12901 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12961 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    13021 tcttgcatcg aggcaaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    13081 ggcgtacgac catgatggct acagggttcg agtgtgacat aggcggtcgc acctttggct
    13141 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc acgttggtgg
    13201 taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttggga
    13261 tgcgtggtaa aacgtcctcg ttgcgccagc tttagcgctc gcgccatgta atactcgtcc
    13321 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13381 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13441 atcaccagat tgccaatcat cttgctcggc acttcgcgct caccggtggc gcgcagctgc
    13501 gatttaatat gattgattgc catttcgacg tcatcggaac tcaccggacg tttttccagc
    13561 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13621 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13681 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13741 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13801 aacagaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13861 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13921 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13981 gcgcctgtgt gcagcagagc gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    14041 aagagatgac gcaactcaat aaagaaacgg tcaaaatcac tcagcaaaac aggctgaatg
    14101 caaaatccag cagtggggtt taccttctgc caggagcgaa aacaccggca agactggaaa
    14161 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgatgca gatggcacca
    14221 ccctgacgct acgtattcag ggcgagtcga atgatccctt gcccgcattc agcggaactg
    14281 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14341 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacagt
    14401 taaagggtat ttctgtagaa cagttagact tcgtacgtat ccacgatatt caacctgtta
    14461 tgcagtaaac gtatttcggg gcgattttgt gcgtctcgca acatctttcc ccgtcatttt
    14521 gttactccgc ttacatcacc ctgattgata gtaaaagttt gcaacaaggg tgaaagtcag
    14581 tacaatccct gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14641 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14701 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14761 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14821 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14881 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14941 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    15001 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    15061 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    15121 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15181 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15241 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatgg cgcagcgaac
    15301 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15361 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15421 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15481 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15541 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15601 gttcgctcta ccggctgggg tggttacctg gttgtaggtt acaacttctg attatgaaaa
    15661 tgccgggatt tattcccggc atttcagatt gttaacaacg tgcgctttgt tcatgccgga
    15721 tgcggcgtaa acgctttgtc cgacccacaa aagcacgtaa attcaatata ttgctgaaat
    15781 aacgtagtcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15841 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15901 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15961 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    16021 gtatggtcgt gatcaatgtg gtgaacagta agttcacgca ggttggaata aacaaactcg
    16081 cgggaacagc gaccgcagac ccacggatat atttttaatg ctttttcgcg atagccgctt
    16141 tctaaccgcg catagttttt tgggatgata gccatgaaga gttacctgga tgaaatgtag
    16201 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16261 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16321 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16381 gtttcagagc caacgcagat gccacataga tggaagatgc agtaccgatg gaaacaccga
    16441 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagaggtac agcatcagga
    16501 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16561 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaatacggt
    16621 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16681 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16741 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcgaaaa cctacgtaca
    16801 caaggataga cagcagcgcc gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16861 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16921 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16981 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    17041 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    17101 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    17161 cgatagcggc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17221 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17281 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17341 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17401 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17461 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17521 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17581 accgccaccg caagggttaa gacgataccc gcgatacctg gcatactcag cgttgcgcct
    17641 ggcaacagcg acataatgcc gacgattaag atcaagttgg caatcagagc actggtcgca
    17701 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17761 aggcaagcct ccagcccctg ttcaatgttc tgcataccca gggttgggcc aatggtacgt
    17821 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17881 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17941 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    18001 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    18061 ttagtgaagt tagacatgat gttaccacca gcgctatcga gcgagatgtt aacctgcggt
    18121 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18181 cgtttgtaca gcacaactgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18241 acgcgaccgg atgccgcagc ggcctggtca acgttggtgt ttaccagacg gaattccagc
    18301 gttgccgtcg cacccagaat ctctttcgca cgcgcagtgt cctgaatacc tggcagttca
    18361 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18421 acacggttac gcaggatatt gatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18481 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctactaat caccaggtcc
    18541 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagta
    18601 atgctcaggc cgtagttgtt ttctttacga acggtggtat acgggatgcc cttttcacgc
    18661 agatcgctgc gcaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18721 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18781 gcgtgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18841 ttgtcaccca taacgcccat taatgcttca cgagcgcgca gctgggtgtc agtggagtcg
    18901 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18961 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    19021 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    19081 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    19141 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19201 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19261 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19321 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19381 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19441 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19501 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19561 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19621 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19681 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19741 ctcaggatcg agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19801 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19861 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19921 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19981 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    20041 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    20101 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    20161 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20221 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20281 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20341 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20401 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20461 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20521 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20581 acaatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20641 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccgcggaatt
    20701 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20761 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20821 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20881 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20941 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    21001 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    21061 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    21121 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21181 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21241 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21301 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21361 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21421 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21481 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21541 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21601 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21661 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21721 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21781 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21841 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21901 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21961 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    22021 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    22081 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    22141 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22201 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22261 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22321 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22381 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22441 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22501 tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22561 gtagcaccac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22621 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22681 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22741 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22801 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22861 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22921 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22981 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    23041 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    23101 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    23161 cttccggctg ggtttctttc gccgcttcgg tgatcccagc aacgtgctgc ata
//
LOCUS       DAZFWL020000068        22931 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFWL020000068 DAZFWL020000000
VERSION     DAZFWL020000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.068"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..912)
                     /gene="stxA2"
                     /locus_tag="ACHKNP_RS14130"
                     /old_locus_tag="pgaptmp_002826"
     CDS             complement(<1..912)
                     /gene="stxA2"
                     /locus_tag="ACHKNP_RS14130"
                     /old_locus_tag="pgaptmp_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU4704963.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAA"
     gene            complement(1003..1079)
                     /locus_tag="ACHKNP_RS14135"
                     /old_locus_tag="pgaptmp_002827"
     tRNA            complement(1003..1079)
                     /locus_tag="ACHKNP_RS14135"
                     /old_locus_tag="pgaptmp_002827"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1043..1045),aa:Arg,seq:tct)
     gene            complement(1093..1169)
                     /locus_tag="ACHKNP_RS14140"
                     /old_locus_tag="pgaptmp_002828"
     tRNA            complement(1093..1169)
                     /locus_tag="ACHKNP_RS14140"
                     /old_locus_tag="pgaptmp_002828"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1133..1135),aa:Arg,seq:tcg)
     gene            complement(1179..1254)
                     /locus_tag="ACHKNP_RS14145"
                     /old_locus_tag="pgaptmp_002829"
     tRNA            complement(1179..1254)
                     /locus_tag="ACHKNP_RS14145"
                     /old_locus_tag="pgaptmp_002829"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1219..1221),aa:Ile,seq:cat)
     gene            complement(1295..2353)
                     /locus_tag="ACHKNP_RS14150"
                     /old_locus_tag="pgaptmp_002830"
     CDS             complement(1295..2353)
                     /locus_tag="ACHKNP_RS14150"
                     /old_locus_tag="pgaptmp_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGU4704964.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRATGVELET
                     ERFEQTVREVQDLASQNG"
     gene            complement(2505..2702)
                     /locus_tag="ACHKNP_RS14155"
                     /old_locus_tag="pgaptmp_002831"
     CDS             complement(2505..2702)
                     /locus_tag="ACHKNP_RS14155"
                     /old_locus_tag="pgaptmp_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4704965.1"
                     /translation="MLKQQDMTETARVVFNELSATAPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(2918..3298)
                     /locus_tag="ACHKNP_RS14160"
                     /old_locus_tag="pgaptmp_002832"
     CDS             complement(2918..3298)
                     /locus_tag="ACHKNP_RS14160"
                     /old_locus_tag="pgaptmp_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4704966.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSTTKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(3317..4306)
                     /locus_tag="ACHKNP_RS14165"
                     /old_locus_tag="pgaptmp_002833"
     CDS             complement(3317..4306)
                     /locus_tag="ACHKNP_RS14165"
                     /old_locus_tag="pgaptmp_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4704967.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVLPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            complement(4358..4615)
                     /locus_tag="ACHKNP_RS14170"
                     /old_locus_tag="pgaptmp_002834"
     CDS             complement(4358..4615)
                     /locus_tag="ACHKNP_RS14170"
                     /old_locus_tag="pgaptmp_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704968.1"
                     /translation="MNNRTTTVSPEQLRRQAQEMLRCAEQMEKTSVAKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFETLVKEFTA"
     gene            complement(4612..6012)
                     /locus_tag="ACHKNP_RS14175"
                     /old_locus_tag="pgaptmp_002835"
     CDS             complement(4612..6012)
                     /locus_tag="ACHKNP_RS14175"
                     /old_locus_tag="pgaptmp_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU4704969.1"
                     /translation="MTSQNTPAWRNDDLEGAVIGAFFLRGADPEVMDILATLSADVFS
                     ARQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMASLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKTERLF"
     gene            complement(6009..6887)
                     /locus_tag="ACHKNP_RS14180"
                     /old_locus_tag="pgaptmp_002836"
     CDS             complement(6009..6887)
                     /locus_tag="ACHKNP_RS14180"
                     /old_locus_tag="pgaptmp_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4704970.1"
                     /translation="MMTFNLREQQTRLQARMDELRAEIAFAQKGEKPWPYRSCWMREG
                     RGCCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSLKAEELTDNA
                     GIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVSMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADDVIEHYASLDLLIIDEV
                     GVQFGSAAEMAILQEIINARYEGILPTILISNLSPEELWAFISPRIADRITNGGRNWL
                     SFNWPSYRSRIGGVAA"
     gene            complement(6898..7806)
                     /locus_tag="ACHKNP_RS14185"
                     /old_locus_tag="pgaptmp_002837"
     CDS             complement(6898..7806)
                     /locus_tag="ACHKNP_RS14185"
                     /old_locus_tag="pgaptmp_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4704971.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETTRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARI
                     REGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFEDFWQHPAAPGGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(7793..8026)
                     /locus_tag="ACHKNP_RS14190"
                     /old_locus_tag="pgaptmp_002838"
     CDS             complement(7793..8026)
                     /locus_tag="ACHKNP_RS14190"
                     /old_locus_tag="pgaptmp_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704972.1"
                     /translation="MIRRVVNSLYHRYNRCPRVGQWFTTSNGHVLRVCLVNTESQMVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGGYASV"
     gene            complement(8023..8685)
                     /locus_tag="ACHKNP_RS14195"
                     /old_locus_tag="pgaptmp_002839"
     CDS             complement(8023..8685)
                     /locus_tag="ACHKNP_RS14195"
                     /old_locus_tag="pgaptmp_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HGU4704973.1"
                     /translation="MIISRPNIDSAISGRYTVSAPYKAGAGGRSPELLEAIYDAPASF
                     LSSMLAHARIMVGWAGEPKGSPVSLSAGTANPVQFTTHEFRSSGGGSFSLLKEAAIMA
                     TVPTSPYLKIEVVNGKAVIFSLHVACHFKRMHQNIVDKIEYLNCSREFFTRNFIPGTY
                     HIYGDSLRGYYITLDGLMMLQLGLSLRTMRYYESCIEAFHEAEISLSHTAFRRNQWEV
                     RP"
     gene            complement(8794..9501)
                     /locus_tag="ACHKNP_RS14200"
                     /old_locus_tag="pgaptmp_002840"
     CDS             complement(8794..9501)
                     /locus_tag="ACHKNP_RS14200"
                     /old_locus_tag="pgaptmp_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4704974.1"
                     /translation="MNTAIFNDKASMTSVEIAELVGSQHKDVKRSIERLMGKGVIRSA
                     PMANFEIINNLGLKRNVAAYVFEGEQGKRDSIIVVAQICPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRITQVEEKVIHVAETVEQIKKGTIREGYAGYRQLKAK
                     TGLSDDKCCNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFYRVMEEAEPRGT
                     RWYHPKMGLFQVIGWQR"
     gene            complement(9583..9816)
                     /locus_tag="ACHKNP_RS14205"
                     /old_locus_tag="pgaptmp_002841"
     CDS             complement(9583..9816)
                     /locus_tag="ACHKNP_RS14205"
                     /old_locus_tag="pgaptmp_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4704975.1"
                     /translation="MLRKLIPQNAIARYIGVTPQAVNLWFSKNSVPSRFVLRVCELVE
                     WKVTPHGLRPDLYPYPEDGIPDSLRKSNGITRD"
     gene            9973..10662
                     /locus_tag="ACHKNP_RS14210"
                     /old_locus_tag="pgaptmp_002842"
     CDS             9973..10662
                     /locus_tag="ACHKNP_RS14210"
                     /old_locus_tag="pgaptmp_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4704976.1"
                     /translation="MKNVKSTENRIAMMLKTKGWSQAELARKLGVSAQSVQYWTTGKT
                     FPRSDKLAQLSVISGYPQSWFLGEDASSTFSSAEKHHTREDSVVFNVLDVEFSCGDGT
                     HVRGDLIDVVRSIELDPEYARRLVGNRAFKNIEIGNARGDSMAPTISPGDLLFLDKTV
                     TYFDGDGIYAFCFDGECYVKRLQKIGSKIMVLSDNPNYQPWSIEKEGMALLYIQSKVI
                     SSVPFNINRFG"
     gene            10810..11511
                     /locus_tag="ACHKNP_RS14215"
                     /old_locus_tag="pgaptmp_002843"
     CDS             10810..11511
                     /locus_tag="ACHKNP_RS14215"
                     /old_locus_tag="pgaptmp_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4704977.1"
                     /translation="MEALQTTPKTCSLNTYNKVLCDDLDLDSFALTIANLLSAVRTFN
                     LLDDTRLKEVGFDVLEFTHEYALAIASTKQQHSIRSGNKIACIRTKREACGLTTTELA
                     RLLDLDEEIILQWESGEFEPTISMLIPLANVLGCDPLSLLSEKNSESAIRVNTTEVHA
                     ESIGARIKSARTKLGLTESDLARMIHTYSDPINDWECGICEVPADHIVPLASALNCDL
                     MWLLTGKSEAKEQQQ"
     gene            11508..11708
                     /locus_tag="ACHKNP_RS14220"
                     /old_locus_tag="pgaptmp_002844"
     CDS             11508..11708
                     /locus_tag="ACHKNP_RS14220"
                     /old_locus_tag="pgaptmp_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704978.1"
                     /translation="MTGNIHDKYEGLCLAPDSFANNIHNLLCAVIVLQMSDNDAIKRT
                     GDEVLEFARCYAEAAAEKELSS"
     gene            11907..12088
                     /locus_tag="ACHKNP_RS14225"
                     /old_locus_tag="pgaptmp_002845"
                     /pseudo
     CDS             11907..12088
                     /locus_tag="ACHKNP_RS14225"
                     /old_locus_tag="pgaptmp_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12094..12666
                     /locus_tag="ACHKNP_RS14230"
                     /old_locus_tag="pgaptmp_002846"
     CDS             12094..12666
                     /locus_tag="ACHKNP_RS14230"
                     /old_locus_tag="pgaptmp_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704979.1"
                     /translation="MQYAEFQAEATANGIQTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDVPNEHGTTDRA
                     VIYNGKHGVMTIYPGPIRFALQQYIEWNLIQKYGEAEGMGRALFLYQKMLITYPDKGF
                     IVSDMGREGLELLLDEMINDLNTHGMPEGQ"
     gene            12851..13039
                     /locus_tag="ACHKNP_RS14235"
                     /old_locus_tag="pgaptmp_002847"
     CDS             12851..13039
                     /locus_tag="ACHKNP_RS14235"
                     /old_locus_tag="pgaptmp_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4704980.1"
                     /translation="MRLYFTIVILLAIIACIYGLLVPFLISMKDTIAVTSGFALAFLT
                     PPCIYAIYKGLSFTKDKK"
     gene            13036..13863
                     /locus_tag="ACHKNP_RS14240"
                     /old_locus_tag="pgaptmp_002848"
     CDS             13036..13863
                     /locus_tag="ACHKNP_RS14240"
                     /old_locus_tag="pgaptmp_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGU4704981.1"
                     /translation="MKKIIFALAIVLPTIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITATDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGVQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKALAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSGANTPFIQIK"
     gene            13904..14275
                     /locus_tag="ACHKNP_RS14245"
                     /old_locus_tag="pgaptmp_002849"
     CDS             13904..14275
                     /locus_tag="ACHKNP_RS14245"
                     /old_locus_tag="pgaptmp_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HGU4704982.1"
                     /translation="MNNQNTQPQIMNYDPNLTSCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVINCAIESLYATLPYEEVLDERTGKTDIMATINIGELICQDEDLSGELW
                     LAGMLISAEIISIEPATNIRL"
     gene            14307..14411
                     /locus_tag="ACHKNP_RS14250"
                     /old_locus_tag="pgaptmp_002850"
                     /pseudo
     CDS             14307..14411
                     /locus_tag="ACHKNP_RS14250"
                     /old_locus_tag="pgaptmp_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            14467..14721
                     /gene="xisR"
                     /locus_tag="ACHKNP_RS14255"
                     /old_locus_tag="pgaptmp_002851"
     CDS             14467..14721
                     /gene="xisR"
                     /locus_tag="ACHKNP_RS14255"
                     /old_locus_tag="pgaptmp_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4704983.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            14755..16041
                     /locus_tag="ACHKNP_RS14260"
                     /old_locus_tag="pgaptmp_002852"
     CDS             14755..16041
                     /locus_tag="ACHKNP_RS14260"
                     /old_locus_tag="pgaptmp_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4704984.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEIGSN
                     AFSRYQSVVRNMLPRIGEKRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPIKGRSVV
                     TVNYYMTTIAGMFQFAADNGYIVSNPFNGLTPLKRSRTEPDPLTRDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAVDALKSQAEMTRLGPQYQIDVKLREFGRTARHECTFVFNPQLVKKCQQVGH
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALSAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKQLTESVPYMPHRARL"
     gene            16025..16822
                     /gene="yecE"
                     /locus_tag="ACHKNP_RS14265"
                     /old_locus_tag="pgaptmp_002853"
     CDS             16025..16822
                     /gene="yecE"
                     /locus_tag="ACHKNP_RS14265"
                     /old_locus_tag="pgaptmp_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HGU4704985.1"
                     /translation="MEPDSEIISLNISCFSGSCRHINCVEGNTTLYALPKPEVVLRWR
                     EQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPR
                     ELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHA
                     ACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQ
                     TTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            16875..17270
                     /gene="yecN"
                     /locus_tag="ACHKNP_RS14270"
                     /old_locus_tag="pgaptmp_002854"
     CDS             16875..17270
                     /gene="yecN"
                     /locus_tag="ACHKNP_RS14270"
                     /old_locus_tag="pgaptmp_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HGU4704986.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            17311..18054
                     /gene="cmoA"
                     /locus_tag="ACHKNP_RS14275"
                     /old_locus_tag="pgaptmp_002855"
     CDS             17311..18054
                     /gene="cmoA"
                     /locus_tag="ACHKNP_RS14275"
                     /old_locus_tag="pgaptmp_002855"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HGU4704987.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            18051..19022
                     /gene="cmoB"
                     /locus_tag="ACHKNP_RS14280"
                     /old_locus_tag="pgaptmp_002856"
     CDS             18051..19022
                     /gene="cmoB"
                     /locus_tag="ACHKNP_RS14280"
                     /old_locus_tag="pgaptmp_002856"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HGU4704988.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(19187..21616)
                     /gene="torZ"
                     /locus_tag="ACHKNP_RS14285"
                     /old_locus_tag="pgaptmp_002857"
     CDS             complement(19187..21616)
                     /gene="torZ"
                     /locus_tag="ACHKNP_RS14285"
                     /old_locus_tag="pgaptmp_002857"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HGU4704989.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIRVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(21641..22741)
                     /gene="torY"
                     /locus_tag="ACHKNP_RS14290"
                     /old_locus_tag="pgaptmp_002858"
     CDS             complement(21641..22741)
                     /gene="torY"
                     /locus_tag="ACHKNP_RS14290"
                     /old_locus_tag="pgaptmp_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HGU4704990.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         5891 a         5866 c         5313 g         5861 t
ORIGIN      
        1 tgcagctgta ttactttccc ataatgtatt gtttattttt ataacgggcc tgtcgccagt
       61 tatctgacat tctggttgac tctcttcatt cacggcgcga acagaacgcg ccccctgatg
      121 atggcaattc agtataacgg ccacagtacc cagtatcgct gatatattat taaaggatat
      181 tctccccact ctgacaccat cctctccccg atactccgga agcacattgc tgattcgccc
      241 ccagttcaga gtgaggtcca cgtctcccgg cgtcatcgta tacacaggag cagtttcaga
      301 cagtgcctga cgaaattctc tctgtatctg cctgaagcgt aaggcttctg ccgtgacagt
      361 gacaaaacgc agaactgctc tggatgcatc tctggtcatt gtattaccac tgaactccat
      421 taacgccaga tatgatgaaa ccagtgagtg acgactgatt tgcattccgg aacgttccag
      481 cgctgcgaca cgttgcagag tggtataact gctgtccgtt gtcatggaaa ccgttgtcac
      541 atcgggcact gatatatgtg taaaatctga aaaacggtag aaagtatttg ttgccgtatt
      601 aacgaacccg gccacatata aattattttg ctcaataatc agacgaagat ggtcaaaacg
      661 cgcctgatag acatcaagcc ctcgtatatc cacagcaaaa taactgcccg gcggggtgtg
      721 gttaataaca gacaccgatg tggtcccctg agatatatgt tcaagagggg tcgatatctc
      781 tgtccgtata ctatttaacg aagagacata actttgttga gtcgaaaagt ctatcgtaaa
      841 ttcccgggaa taggataccg aagaaaaacc cagtaacagg cacagtaccc atttaaataa
      901 tatacacttc atatacaggt gttccttttg gctgaagtaa tcagcaccag acccggcgca
      961 gatataaaaa aggcccgcaa aagcgagcct ggtaaataaa tatggcgcgt tgtactggat
     1021 tcgaaccagt gaccgattgc ttagaaggca attgctctgt ccggctgagc taacaacgct
     1081 gaataccgat aatggaccgc catcgaggac tcgaaccccg cgcagccagc ttcgaaggct
     1141 ggcgctctgt cccgatgagc taatggcggt atgtgatatg gtggcccttg ctggatttga
     1201 accagcgacc tggcgattat gagtcgctcg ctctcaccac tgagctaaag ggccgggcgc
     1261 aggataataa cggtacgtaa ctaatcctgc aatatcatcc gttctgactg gctaaatcct
     1321 gaacttccct gaccgtctgc tcaaaacgtt cagtctccag ctcaacgcca gttgcacgac
     1381 gccccagcgc cagtgcagct ttgactgtcg aacccgaccc catgaaaaaa tctgcaacca
     1441 ggtcacccgg acgactgctc gcgctgatta tctgctgcag catttctgcc ggtttttcgc
     1501 acggatgttt cccgggatag tactgcaccg gtttatgcgt ccacacatcc gtgtacggca
     1561 cctgcgccgt cacgccaaaa taccgccgca gatgcttata ttcactctgc agttccgcat
     1621 actgccggtt cagtgaagta tacgtctcca gcagctggtg gtggggcttt tccagttcac
     1681 cgcgctgatg cttctcttct gccacccggg caaacagcga ctgtaatttc agataatcgc
     1741 tttcgttcgg tagctgccac tgactggcac tgaaccagtg cgacaccatg tttttctttc
     1801 ctgtggcatc cactatctgt tttgccgtta tccccagggc agcacgcgca tcacgaaagt
     1861 aagcaatcag cggagccatc acatgctgtt tcagtgccct gcccttcgcc tcatacccgg
     1921 catctttcgg acgatacggc ccctgataat gttccgcgaa cagaatgcgc tctgtggcgg
     1981 ggaaatacgc cctcaggctt tccttgttgc acccgttcca gcgtccggac ggcttcgccc
     2041 agataatatg gttcagcaca ctgaagcgtt cacgcatcat gatttcaatg tcagatgcca
     2101 ggcgatggcc acagaacagg taaagacttc cggcaggttt cagcacccgc cagaactgcg
     2161 ccagacactg gtccagccat ttcaggtaat cctcatcacc cgtccactgg ttatcccagc
     2221 cctcaggctt cactttaaag tacggcgggt ccgtgactat caggtcaaca gaattttcgg
     2281 gtaacgaccg gataaattcc aggcagtcgg cgttgattaa ctcacaactg gatattttta
     2341 cagtattaaa catggatcat taagcctgtc tctgataggc tcataccgct tttgcgcaaa
     2401 gcagatgggc ctgaggtttg cttgtgaccc caacgcatga gcagatggct ggtgagtgcc
     2461 cctaacaccc accagccgcc catttaccac aaataaaaaa gccttcagga ctgaaggcgt
     2521 ctgtaacaac caaactgata gtctgccaga cccgccataa caagctgggt cagtattagc
     2581 tggcagcgtt cgcgtgaaag gtacgtattc tgtgcaatct ccccgactgt cgccggtgcg
     2641 gtggcactta attcattaaa caccactctg gcggtttctg tcatatcctg ctgtttcagc
     2701 atgtcttttt cccttttccg gttaacgtga cacaccaata actcttgtcg aaaaagccag
     2761 caagctgaaa gaccggtatt caccgccacc agcgcgttta acgtactggt ccgatttcag
     2821 gcataaaaaa cccgcctgac ggcggattta agctgtgtgg caaagtaacc actcttaaca
     2881 tactgacata ctttttgcgg accgcactaa tcatttttta cttttttagc agccagtcgt
     2941 ccatctccag tcttaccccc agcacagaca aacatccgtc aataaaccct tcggctatct
     3001 gcatctcaat tcgtattgct ttttcgcttt tctttctcgt cctggctatc tgtctttttg
     3061 atattcgcaa caaataatga gcaatgagaa gcgaatactc ctcaggtttt ttctgcttca
     3121 gacgtgcaag acagttttca atgataagtc cgtcatcatc gcagcaggct ggacgtggtt
     3181 tagtggtaga tggtaaaagg cctttgaatc cggcagcgat cggagaatag tccaccccgg
     3241 tgttaccact tgccgcccat gccccccagc gttcaagaac catctgaata tcacgcatca
     3301 actttctcca caaaaatcag gacagcacac caatcgccag tgcgcgatcg ataaaacgaa
     3361 atatcagctc cagttgggaa ccatacttct cttcaaatgc cacggtatcc gcatgcagtt
     3421 cgtcatggtg ttttctgcac aaaggcaaca caaaaaggtc atgcgctttt gtacccattc
     3481 caccctgacc atggccaatc aggtgatgtg gatcgtcggc tggcttacca caacacgcgc
     3541 aaggctgtgt cttaacccag cgcgtgtact tttcattaac ccagcgacga cgtttggggc
     3601 gtaacataaa agactccggc gactccggat ccactttcag cgccagcacc tttttcgctt
     3661 tatcctggat aatgctggtg gcaggaaccg aaggcacaag gtcactctcc cgggtgacag
     3721 acggcaaaac aggcttcggt aatctcaggg ccttacgggc tgcactttcc ggtaaggcat
     3781 ccgccaggtc attacgaacc agccaccagc acagttcagg cattgtcaca acgtgactgt
     3841 catcaaaacc gagatcacga cggactacgg acaacaccca gcgggcacag ttatccgttg
     3901 ccattgactc cagacgttcc gtgaactgat cgcgaagcag gttatcgcag tgccagcaca
     3961 gacggattgc gcccggcgcg tgtcgcattg tggtcatgtt ctcgctgtgc catccggaat
     4021 gaggccactg gcagcctttt tcacgaagta accagctctc aaggcattcc acgccaccag
     4081 cacgacggat caccgcctca tggcggaaca cggcccgaac ggcaggatca tccgccagcg
     4141 gttgtgatgc tgccggaacg gcaccactgg caaaagatga ataacgttcc ggctcaggct
     4201 ccagcaggac acgcccctgc ataaacaggg gcatcagctc tgaaccgggt ctgaacaaga
     4261 cgatccccat acgcggggca atttcagggg tcagcagtgc tctcacggtc acctcagcga
     4321 acggtattgc atgaacgcag gagaaaaaat tcagccatca cgcagtaaac tctttcacca
     4381 gcgtttcaaa ctggcttacc tggccttcca gttccgccac gcaatccacc agctcatcca
     4441 ccgccttttg tgtgcgatgt tttgcctgca gcagatcacg aagcgccgga gtaagctgct
     4501 tgcggagcgt atcttttgcc acgctcgttt tttccatctg ttcagcacaa cgaagcatct
     4561 cctgcgcctg ccgacgaagt tgttccggtg aaacagtggt ggttctgttg ttcaaaataa
     4621 acgctccgtt ttactacccg acatgcggtt attgctgtat ctgcgcggat tgcccggcgt
     4681 catgggagtg gaaagaaccc gggcactctc ctggtccaca ggcagaaaat gcccgttatg
     4741 aaaacgccgg taaatggtac ccagcgtgcc attacgctgt ttcgtgatgt tgatttcagc
     4801 tatgcctctc gcctgtgtct ccgggttgta cacctcatcc ctgtaaagca tcagaatgat
     4861 gtctgcatcc gcctctattt cccctgagtt tttcaggtcc gagttcatgg ggcgtttatt
     4921 gggtctggat tccacgccac gggagagctg gctcagagca atcagcggaa aaccgccgga
     4981 ttttgccagg cttttaagtc cctttgagat ttcccccaca gcaaggtcgt gacgccccgt
     5041 gctgcgggtt ttaatcaggc cgaggtaatc gaccaccacc agcgccgttt ccgggtgttt
     5101 catccggtgg tgcctcgtgg ttgcacatat ctcatcaatg gtcaggtttg cctggtccac
     5161 catccagata ttacgccccg tcattcgtcc cacgccctgt gagaaacgcg cccagtcttc
     5221 atcttcaaaa cgggcaacag acttaagacg ggataccggc attcccccgg cagcagacac
     5281 catacgttca ccaatctgaa tgttcgccat ctccatggtg aacagaagca cgccatgccc
     5341 ctgctcagtc accttgtcga tgatatccag cgcaagttcg gttttcccca tcgaaggacg
     5401 ggccgcaatg aataccaggt cgcctggctc cataccgccc gtttttgcgt ccagttcatc
     5461 aataccggtc atcagcgccc tggatttctc cagtccctga ttgcggcatt caacacggtc
     5521 gaccacttcc ggaaggacat catcaatgtg aaccggctga atgacgccct ttccggtcga
     5581 cagtgaggcc atcatgttct gcgcatcctt cagggcatcc tcggctgctt cacaggtatg
     5641 cgcatcccgt aaattctgta atgcttcagt cagtgttttt tctgcatcgc gcagtgcggc
     5701 attacgccgc aacgctgcga catagtgctc cagtgaagac ttcacccagg ttttgcgtcc
     5761 ggtgtcggta atcaccgggg caagttccgg catctcattg cacagcagta cggggtcaat
     5821 gacgccggat acacgtgcct gtctgcaaat tcccgcgtaa atatcccggt actgtcgtgc
     5881 agaaaaaacg tccgccgaca atgtggccag aatatccatc acttccggat cagccccacg
     5941 cagaaaaaac gcgccaatga cagcgccttc caggtcatcg ttacgccatg ccggggtgtt
     6001 ctggctggtc atgcggcaac acctccgata cgagaacggt agctgggcca gttaaacgac
     6061 aaccagttgc gcccgccatt ggtgatcctg tcggcaatcc ggggactgat gaacgcccac
     6121 aattcttccg gtgaaaggtt gctgatcaag atagttggca aaataccctc ataccgggca
     6181 ttgataattt cctgcaaaat ggccatttca gccgcactgc caaactgaac gccgacttcg
     6241 tcgatgatca gcaaatccag tgacgcataa tgctcaatga cgtcatccgc tgttttttca
     6301 ctgtcattcc gccagcagtt tttcacagca cgggtaaggc gcatcacgtc ggtgatctcc
     6361 acactggcca gatagttacg gatgatgtgt tttgccattg ataccgccag atgatttttc
     6421 ccggtaccgc aactgccggt cataacaaga ctggtaccgt tctccagcat atctggccag
     6481 ttctccgcat agcggcgaca ggccgcaaga tttctggctg cgtcaggatt aacctccaga
     6541 taattatcaa actcgcagtc ccgaaaacgc agagcaattc cggcgttatc agtcagttct
     6601 tccgccttga gggacgacag ctccatggtc aaatcgttgg cctcagctat caagcagtca
     6661 gggcagcatg aaattttttc tctgtcctcg ccattacgat cgctccacac cagaatatgt
     6721 gtgtggtatt cgccatgttt ttcgcaacac ccgcgccctt cacgcatcca gcaggaacga
     6781 taaggccatg gtttttcgcc cttctgagca aatgcaatct ctgcccgtaa ctcatccatc
     6841 cgcgcctgta gtcttgtttg ttgttcacgc aggttaaacg tcatcatcgc tgtcacctca
     6901 gaatgtcaat ttgtcactgg atttaccgaa tttgtcagac atggctccca ggccagccag
     6961 gacatcgacc tgtcgctgtc gcccacctcc gggagctgct ggatgttgcc agaaatcttc
     7021 gaagtgacga tcgggtccaa agaacgtcga cgcctgcttc acgaactggg tgccggtatt
     7081 tcctgagaca cgcacccagg cagcatagcg tttcacaccg ttgagcatgg tttctggtgt
     7141 cacaccttcc ctgattcggg ctttccaggc tttgaaggct gctgacttgg aattaccacc
     7201 agcacgtttg ggatattcct gccaggcctg ttcaaattcc ggtgaatatt cctgtcgggc
     7261 agaacgcgct ggtgcagacg cgtcagcgga tgcgccaata gtgtttttac tctctgtagt
     7321 attctctgaa gtaatctctg ttgtattctc tgtaagatcg aaattggttt tcccttctcc
     7381 gcggcgagtg gtttcccgtg tccgcggtga aggctttccc tcctccgcga aattgggttt
     7441 tacagtttcc cgaaaacggg tttccccatt tcgggaaaac tgattgtttt cattgataat
     7501 ttcattaagg cgctcacaat ctatacggta gaacattttg tgctcaagac gcttgttggt
     7561 ttcaaccaaa atgcctctgg acacaagatg cttacgcgct acagcctgtt gttcaaatgt
     7621 aagtccagtt tcgtgttgta tctcttcacg cgttttatgt acgccttccg ctgcatgtgc
     7681 tttatcctgc cagtaaaaaa tctgaccaaa gaaaataaca gcgtgcggac ttcccatgta
     7741 tttaacgagc ccagggtaat aagcaaccgg atgcccaaaa tcgagcagaa gatcagacgg
     7801 acgcatagcc acctcccaga cgcttaaaca ttttcccgga ctgaaaagcc accagcggat
     7861 aactcagggt atgagtacgt ccctgaacct ggcagacaac catctggctt tctgtattga
     7921 ccaggcaaac ccgcagaacg tggccgttgc tggtggtgaa ccactgcccc acacgggggc
     7981 aacggttgta tcggtgatac agggaattaa cgacgcggcg aatcatgggc gcacctccca
     8041 ttgattacgg cggaaagcgg tgtgactgag gctgatttct gcctcatgga atgcttcaat
     8101 gcagctctcg tagtaccgca ttgtgcgtag acttaaccca agctgaagca tcatcagacc
     8161 atcaagggtg atgtaataac cacgcaggga gtcaccgtag atgtgataag tacccggtat
     8221 gaaattgcgg gtaaaaaact cgcgtgagca gttcagatac tcgattttgt cgacgatgtt
     8281 ctggtgcatg cgcttaaagt ggcaggcaac atgcagggag aaaataacgg ccttgccgtt
     8341 gacaacttca attttcaggt atggggaagt tgggactgta gccatgatgg cagcctcctt
     8401 gagcagtgaa aaacttccac caccggagct gcgaaactca tgggtggtga actgaacagg
     8461 gttcgcagta ccggcgctca aggaaaccgg cgagcctttc ggctcccctg cccagcccac
     8521 cataattctg gcgtgcgcga gcatggacga taaaaaagac gctggcgcgt cgtatatcgc
     8581 ctcgagcaat tccgggctgc gacccccggc acccgcttta taaggtgcgg agacagtgta
     8641 acgtcccgaa attgcagaat caatatttgg tcttgaaatg atcatatagc tgctgatatc
     8701 tttagaactg ttcttggatg tttcggagcc gttttatgcg aaacagctcc ccgttattga
     8761 tgttgagtga gccgggttac tcccggcttt ttttcaccgc tgccaaccaa taacctgaaa
     8821 taaccccatt ttcgggtgat accagcgagt ccctcgcggt tctgcttcct ccataacccg
     8881 ataaaaagca gccataaacg gttccacagc aacaattgcg cgacgtgaca acaatccgtc
     8941 cggcgtcatg aactcatgtg tgtctgtagg aatttgatag gcgttcacca gattgcagca
     9001 tttatcatct gacaaaccgg tttttgcttt cagttggcga tatccggcat agccctcacg
     9061 aatagtgccc tttttaattt gctcaactgt ttcagcaacg tggatgactt tttcttccac
     9121 ctgagtgatc cgtttctgtt ggcgaacggc ttcaagagcc atcgcggcaa ccatttcgat
     9181 ttcgctcatt ggcttacgga tctgttcttc cagttcacgc cagcggtcta ccaagcgggc
     9241 ggtgaattca gggcaaatct gcgcgacaac aatgatactg tcgcgtttgc cttgttcacc
     9301 ttcgaatacg taagcagcta catttctttt caatcctaag ttgttgatta tttcgaaatt
     9361 cgccattggc gcacttcgga taactccctt ccccatgagt ctttcaatgc tgcgtttaac
     9421 atctttgtgt tggctgccca ccagctctgc aatctcaacg ctggtcatgg atgctttatc
     9481 gttaaaaatt gcggtgttca tctgattgct ccttgaggat gacatttcag aactcacgcc
     9541 aaaacgtatt catcgtgaat tactgcttgt gacagtcagt ctttaatctc tggtaatacc
     9601 gtttgatttt cgtaacgaat caggaattcc atcttcggga taagggtata gatcaggtct
     9661 taacccatgc ggagtaacct tccattcaac caattcacac acccgtaaaa caaaacgaga
     9721 aggaacggag tttttagaaa accacagatt caccgcttgt ggcgtaacac cgatgtatct
     9781 cgctatggcg ttttgaggga tcaatttacg caacatgtcg taatcattca gttttatcac
     9841 agcgcagctc caacattaac tttacaaatc aagaatacat caagaataaa ttaacatgca
     9901 agtttcaaaa ggatcgaata cactaaaatc aagttaatat ttatgtgtat aaagcttcga
     9961 caggaactca ccatgaagaa tgtaaaaagc acagaaaatc ggatagcaat gatgctgaaa
    10021 acgaaaggct ggagtcaggc tgaactagcc cgtaagctgg gtgtaagcgc gcaatcagta
    10081 caatactgga ctacaggaaa gacgtttccc agaagcgata aacttgcaca gctatcagta
    10141 attagtggtt atccacaatc ctggtttttg ggtgaagatg cctcatcgac attctcttca
    10201 gctgaaaaac accatacaag agaagacagc gttgtgttca atgtactgga tgttgaattc
    10261 agttgcggcg acggaactca tgtacgggga gatctaattg atgtagtacg ctcaatagaa
    10321 cttgatcctg aatatgcccg ccgcctggtc ggtaacagag cattcaagaa catagaaatc
    10381 ggtaacgcca gaggggacag catggcccca acaatctccc cgggagactt actgttttta
    10441 gataaaacag taacttactt tgacggcgat ggcatttatg cattttgctt tgatggcgaa
    10501 tgttacgtca aacgacttca aaaaattgga agcaagatca tggtcttatc agacaatccc
    10561 aactatcagc catggagcat cgaaaaagag ggaatggcgc tgctctatat ccagtcaaaa
    10621 gtaatctcat ctgtaccatt caatatcaac agatttggtt agttattgat tttaaattaa
    10681 attattggtc atccatcaca aaaaaatcaa gtttatcaat ttttaattga cagtcgtttt
    10741 cctgacacat aatatctcac catcaattat atattgatta acttcaactt agaattgcat
    10801 ggtgatgata tggaagcctt acaaacaaca ccaaaaacat gtagcctcaa tacttacaac
    10861 aaggttttat gtgatgattt agatctcgat tcttttgcat taaccatcgc aaacctgctc
    10921 agtgctgttc gcaccttcaa cctcctagat gatacgcgat taaaagaggt tgggtttgat
    10981 gtgctagaat ttactcatga atatgcttta gcgatcgcat cgacaaaaca acaacattct
    11041 atccgatcag gcaacaagat agcctgcata cgcaccaaac gtgaagcctg cggcttgacg
    11101 actaccgaac tagccagact actcgatctt gacgaagaga ttattctgca atgggagagt
    11161 ggagagttcg agccaaccat cagcatgctt atccccctgg caaacgtcct gggatgcgat
    11221 ccgctttctc tactaagtga aaaaaacagc gagtcagcta ttcgtgtaaa tacgactgaa
    11281 gtccatgcgg aaagtattgg cgcacgcatc aaaagcgccc gtacaaagct gggattaacc
    11341 gaatctgatc ttgcccgcat gattcatacc tacagtgacc ccataaacga ctgggaatgc
    11401 ggcatctgtg aagttcctgc tgatcatata gtaccactgg ccagtgcgct taattgtgac
    11461 ctgatgtggt tgttaacggg aaaatcagaa gcaaaggagc agcaacaatg actggcaata
    11521 tccatgataa gtatgaaggc ttatgcctgg caccggattc ctttgcaaac aatatccata
    11581 atttattatg cgcagttatc gtattacaaa tgtcagacaa cgacgcaata aaaagaacag
    11641 gtgatgaagt tcttgaattt gcacgttgct atgctgaagc agcagctgaa aaagaactat
    11701 ccagttaaat agaacaagtc atctccagat actatattac ggcttaatcg ccggggatta
    11761 tcacacactt aatccacagg aggttttatt atgactttta taaaacataa ggcatcacac
    11821 aaaacagcct gccttattgc acaacacggg aaaaatcaca tgcatattgc ctgcttgttt
    11881 ctgcgtaaag catacgggag ataataatgc atcagaaaac agcagaacac gaacaaacca
    11941 gagtattgct gaccatcaaa aacgggaaag taatattaat tcgtcatgtt catgacgatg
    12001 aatttgtaga aatctttcaa cattcctgtt tattgcagaa aaggcaggat atgacgttat
    12061 tgcaccagca gatgaagatg aggaataaat ttcatgcaat acgctgaatt ccaggctgaa
    12121 gcaacagcca atggtataca aacaggcagt atgactattg attatcacga cgccatacgc
    12181 cgtctggatg ctggagaatt cgatactcct aatgtgcgag gtttacgtat ccttcagtgt
    12241 ctcgcgcaag ctgacgaagc aggattactg ggaaaacttc cggttgagat gaaggttgcc
    12301 cagtggcgat ggttgtatgt gacgacattc atcaacgaag aagaagacaa gaacggcaca
    12361 attgatgtcc cgaatgaaca cggaacaaca gatcgcgccg taatatataa cgggaagcat
    12421 ggggttatga cgatatatcc cggccccatt cggtttgcct tacagcagta tattgaatgg
    12481 aatttaattc aaaaatacgg cgaggcagaa ggaatgggta gagcgctgtt tctttatcag
    12541 aaaatgctca ttacataccc agataaaggt ttcattgttt cagacatggg gcgagaaggg
    12601 cttgaactcc ttctggatga aatgattaac gacctgaata ctcatggtat gccagaaggc
    12661 cagtgacaca ttaaatatta agaagaatat aattcttccg ttttttacta accatttata
    12721 tgaaaaacaa ccgtgaatta agcagagtaa aactgctttt aatccttgcc acagtactga
    12781 caataacaga aatcattatt ctctttattg cgctgtctgt cagttaaaaa tatcgggata
    12841 ccacatacca atgagactgt atttcacaat agtaatttta ctggcaatta tcgcatgcat
    12901 ttacggatta ctcgttccgt tccttatatc catgaaggat acgatagcag ttacttctgg
    12961 ctttgcactg gcgtttctga ccccgccctg catttatgcc atttacaagg gtctttcttt
    13021 cactaaggat aaaaaatgaa aaaaattatt tttgctttag ccattgttct gccgaccatt
    13081 ggccttgtcg gctgcgatcg cgttgaaccc ggtaatgttg gcatcaaagt aaataaactg
    13141 ggcgacgata aaggcgtcgg tgaggtggtc ggtgttggtc gctactggac tggctggaat
    13201 actgaagttt acatcttccc aaccttcaaa caaatgaaga cctacgatga gccgttcagc
    13261 ttccagatga gtgacggcac aaccatcggc tatcacatcg gtgttgccta caaagttgat
    13321 ccatccaaag ttaccacggt gtttcagacc taccgcaaag gcgtggatga cattaccgcc
    13381 actgacctgc gccagaagat cgccgacgca ctcaatcgac tggccagcaa aatgaccacc
    13441 gacaaattta tcgacggcgg caagtctgaa ctactggatg ccgcacttaa agacattcag
    13501 gaagagatga cccccatcgg cgttcaggta atgagcctct cttatgtagg taaaccggaa
    13561 tacccgccaa ccgttatcga cagcattaac gccaaagtca cggcaaacca gaaaaccctg
    13621 cagcgcgaac aggaagtcaa gcaacgtgaa gcggaggcca acatgctgcg tgcagaagct
    13681 gccggacagg ctgatgccat tcgaacaaaa gccctggcag aagccgatgc cattcgttta
    13741 cgcggcgaag ctctgcgcca gaaccctggc gtcatggagc tggaagccat caacaagtgg
    13801 aacggtacac tgccgcaata catgaccagt ggtgccaata caccatttat ccagattaaa
    13861 taacttacac gcccggcaaa ccgccgggtt aatggaaaat cagatgaaca accagaatac
    13921 tcaaccgcaa ataatgaact atgacccgaa tctgacgtcg tgcggacgca tggcaaaaca
    13981 aaccgttcga ttaactttcg gactatggga ataccgcgaa acattcgaag ttactgtcgg
    14041 cggcaatctg accggactgg atgttatcaa ttgcgctatt gaaagcctgt acgcaacgct
    14101 gccttatgaa gaagtcctgg atgagcgcac agggaaaacg gatatcatgg ccaccattaa
    14161 tattggcgaa ctgatatgcc aggatgaaga cctgtccggg gaactctggc ttgccgggat
    14221 gcttatctca gcagaaatta tcagcattga acccgctaca aacatacggc tctgaagttc
    14281 tcactattca gagagcagga gaaaaaatgt tcgctctgat taatcaggga caactgtata
    14341 ccgacagtgc cggttacccg gtaaaaattg ttcgctgcat aaacaacacc gtgttgtaca
    14401 gaagatgtga agcgaatgtt tgaagtgtgg tgccactgcc gtggatgaaa gttttatgag
    14461 gttggcatgc agacaatcat ctatcagata acccccagca aatggtgtac ggagagagtc
    14521 ctcattgcat caacagggct aaagcctggc accatcgagc gagccagaag aaagtcatgg
    14581 atgcagggaa aagaataccg ccattacgct gtagaaggtg atccggggca ttacagtgaa
    14641 tgcctgtaca acatcgaaga aattatgcga tggatcgaaa accagaaaca accaggtgcc
    14701 aaaaatgcaa gttccggtta acctgttaat gctcctggac gtctgggagg ttttatgagt
    14761 aacgcatcat acccgacagg cgttgaaaac catggaggat cactccgtat atggtttcac
    14821 tataatggca aacgtgtcag agaaaacctc ggtgttcctg acaccgccaa aaaccggaag
    14881 atcgctggtg agcttcgcac ttctgtttgt tttgcaatca gaatggggag tttcgactac
    14941 gccgcgcagt tccctaattc ccctaacctg aaacactttg gtctgggaaa aagagagata
    15001 accgttaagg cactttcgga aaaatggttg gaccttaaga aaattgagat aggaagtaat
    15061 gcattcagtc ggtatcaatc cgtggtgaga aacatgcttc ctcgcatagg ggaaaaacgt
    15121 cttgcttcgt cggtaacaaa ggaagattta ctgtttatca ggaaagattt attgaccggg
    15181 tatcataatc tttctaacgg aaaaacaacg ccgattaaag ggaggtcagt agttacggtt
    15241 aattactaca tgacgacaat tgcaggaatg tttcaatttg cggctgataa cggctatatc
    15301 gtgtcaaacc catttaacgg cctgacacca ttaaagagat ccagaacaga accagatccg
    15361 ctcacacgtg acgaatttat tcgttttatt gatgcctgtc accatcaaca aacaaaaaac
    15421 ctgtggtcct tagcagtata cacaggcatt cgtcacggtg agctaatatc tctcgcctgg
    15481 gaggatattg atttaaaagc taaaacaatg actatccgtc gtaattatac aaaactcggg
    15541 gagttcactc taccaaaaac agaagcggga actgatcgtg ttattcatct tgttcaacca
    15601 gctgttgatg ccctgaaaag ccaggctgaa atgacaagac ttggccctca gtatcaaatt
    15661 gacgtcaagc ttcgggagtt cggtcgcacc gcacgccatg aatgcacgtt tgtttttaat
    15721 ccgcaactgg taaaaaaatg ccagcaagtc ggtcaccact ataaagcaga ttccatcaga
    15781 gattcctggg catctgcatt aaggcgagca ggactgcggc acagaaaagc ctatcagtct
    15841 aggcatactt atgcctgctg ggcattatcg gcaggagcga atccaagctt catagcaaac
    15901 caaatgggcc atgcaaatgc acaaatggta ttcaacgttt acggagcatg gatgaaagat
    15961 aacaatatcg ggcaaataga actactcaat aagcagttga cggagagtgt cccatacatg
    16021 ccccatagag ccagactctg aaataatatc tttaaatatc agttgtttta gtggtagttg
    16081 ccgtcacatt aactgcgtgg agggcaacac cacgctttac gccctgccga aacccgaggt
    16141 tgtcctgcgc tggcgtgaac agaccacaga tgacttccgc ttctgtttta agtttccggc
    16201 gaccatttcg catcaggcag cattacgaca ttgcgatgat ttagtgactg aatttttgac
    16261 ccgcatgtca ccgttggctc cgcgcattgg gcaatactgg ctgcaactgc ctgccacatt
    16321 cggcccacgg gagctgcctg cgctttggca ttttctcgat tctctccctg gcgaatttaa
    16381 ttatggtgtg gaagtccgcc atccacagtt tttcgccaaa ggggaagagg aacaaacgct
    16441 taatcgcggt ttacatcagc gcggcgttaa tcgggtgatt ttagacagcc gcccggttca
    16501 tgcagcatgt ccacacagtg aagctattcg cgacgctcaa cgaaaaaaac ctaaagttcc
    16561 ggtacatgct gtactgacgg cgacaaatcc gctgatccgt tttatcggta gtgatgatat
    16621 gacgcaaaac cgggaattat ttcaggtctg gttacagaaa ttagcgcagt ggcatcagac
    16681 cactacgcct tatctttttt tacatacgcc agacattgcc caggccccgg aactggtaca
    16741 taccctgtgg gaagacttac gtaaaacgct tccagagatc ggagcagttc cggctattcc
    16801 acagcaatct tctcttttct gaatttgcca cctatcatag acaggtgcca tcggccattt
    16861 taaagggagt ttgtatggta agcgcgctat atgccgtttt aagtgcgttg ttattaatga
    16921 agttctcttt tgatgtcgtt cgcttgcgtt tgcagtaccg cgttgcctat ggtgacggtg
    16981 gttttagcga actgcaaagc gctattcgca ttcatggtaa cgcggtggaa tatattccca
    17041 tcgcgattgt gctgatgctg tttatggaaa tgaatggcgc agaaacctgg atggtgcata
    17101 tttgcggcat cgttttgctt gctggtcgtc tgatgcatta ttacggtttt catcaccgtc
    17161 tgtttcgctg gcgacgttcc ggcatgagcg ccacctggtg tgcgctgttg ctgatggtgc
    17221 tggcgaatct ttggtatatg ccctgggagt tggttttctc cctgcgttag cgcacaatac
    17281 gccactttct ttttcccgga tttttacgtt atgtctcacc gcgacacgct attttctgcc
    17341 cctatcgcca gactgggcga ctggaccttt gatgaacggg tagctgaagt cttcccggat
    17401 atgatccagc gttccgttcc cggctattcc aatattattt ccatgattgg tatgttagcc
    17461 gagcgcttcg ttcaacctgg tacgcaggtt tacgatctgg gttgttctct gggcgcggcg
    17521 acgctctcgg tgcgtcgcaa cattcatcat gataattgca aaattattgc catcgacaac
    17581 tccccggcga tgattgaacg ctgccgtcgt catattgacg cctataaagc ccctacgcca
    17641 gtagacgtca ttgaaggtga tattcgcgat atcgccattg aaaacgcatc gatggtggtg
    17701 ctgaatttta ccctgcaatt cctggaacct tccgagcgcc aggcattact ggataaaatt
    17761 tatcaaggac tgaacccggg cggcgcgctg gtgctttcgg aaaaattcag tttcgaagat
    17821 gccaaagttg gtgaactgtt gttcaacatg caccacgact ttaaacgtgc caacggttac
    17881 agcgaactgg agatcagcca gaagcgcagc atgctggaaa acgtgatgct gaccgattcc
    17941 gtggaaaccc ataaagcacg cctgcataaa gccggttttg agcatagcga gctgtggttc
    18001 cagtgcttta actttggttc actggtggca ttaaaagcag aggacgctgc atgatcgact
    18061 ttggtaactt ttattctctg attgccaaaa atcatctttc acactggctc gaaacgctgc
    18121 ccgcgcagat tgctaactgg cagcgcgagc agcagcacgg gctgtttaag cagtggtcca
    18181 acgcggtgga atttctgcct gaaattaaac cgtatcgtct ggatttattg catagcgtaa
    18241 ccgccgaaag cgaagagcca ctgagcaccg ggcaaattaa acgcattgaa acgcttatgc
    18301 ggaacctgat gccatggcgc aaagggccgt tctcactgta tggcgtcaac atcgataccg
    18361 aatggcgttc cgactggaaa tgggatcgtg ttctgcccca tctttctgat ttaaccggac
    18421 gcaccattct ggatgtcggc tgcggcagcg gttatcacat gtggcgcatg attggcgcag
    18481 gcgcgcacct cgcggtgggg atcgacccta cgcaactgtt tctgtgccag tttgaagcgg
    18541 tgcgtaaact actgggtaac gatcagcgcg cacatttgtt accgttaggt attgaacagc
    18601 ttccggcact gaaagccttt gataccgtct tttcgatggg cgtgctttac catcgccgtt
    18661 cgccgctgga gcatctctgg cagttgaaag atcaactggt gaatgagggt gaactggtgc
    18721 tggaaacgct ggttattgat ggcgatgaga acacggtgct ggtaccaggc gatcgttacg
    18781 cgcaaatgcg taatgtctat ttcatccctt ccgcgctggc gctgaaaaac tggctgaaga
    18841 agtgtggttt tgtagatatc cgcgttgtgg atgtgtgcgt taccactact gaagagcagc
    18901 gacgcactga atggatggtt accgaatctc tgtccgattt cctcgaccca catgatccga
    18961 gtaagacagt tgaaggttat cctgcgccta aacgcgcggt gctgattgcg cgcaagccgt
    19021 aaaggtctgg cattactgcc ggatgcggct tgaatgcctt atccggccta caaacccttg
    19081 ctaattcaat ctattgcagg gactatgtag gcctgataag catagcgcat caggcagttt
    19141 tacgtttgca taacctcagc gcccgtttcc gggcgctatt cacttcttac gcctgaacag
    19201 caggcttatc aaatgccgtt aacttcggtg cgttacccgt atatttttcg atatacacca
    19261 gcgcagagtt accggcacag gcatttgcca gctgcgagct ggggatatcc gccgttaaca
    19321 cgttcgcact gccgttttta cacaagccgt tttccagatc tggccatgca ccttcatgca
    19381 cgcataccac gccttttttg atcccgtcag tcaccaccgc gcctgtcaaa atctgaccac
    19441 gtttgttcca gacacgcacc agatcgccat tcgcaatacc aaatcgagca gcatcttcgg
    19501 tgtgaatagt gatcggttca cgatcggcaa ccgcatattt tttacgcagt tccgcatagt
    19561 taagctgact atgtaaacgg tgtgccggat gtgcggtcag aagctgcaac tgcttctcgt
    19621 cggcggtacc cttccactca tcaggcgcaa gccaggttgg atgtgccggg caatccttat
    19681 agccaaattt ttccagcgtt ttggagtaaa tctcaatttt gccgcttggc gtacccagcg
    19741 catttttcac cggatcggcg cggaaatcag cataacgaac gtactgttcg ttcttctcgc
    19801 tgcggcgcat ttcgatcagt ttattttgct gccagaaggc attaaacatc ggcatagtca
    19861 cgcgttgcgc acgagcacct ttctgagcgg catcatagaa aaatttcagc cacgccattt
    19921 catctttacc ttcggtatag atctcttttc cgccaggttt cagtaattca gcaagatcgg
    19981 caaatacgtc aaaatcgtta cgcgcttcaa attgcggagc aacagcctgc ttcatcggca
    20041 caatatgctg gttgctgtaa tcaccggtca tcgtcaagtc attgcgctca aacgatgtgg
    20101 tgatcggtaa tacgatatct gcatgtttag cagctgcggt ccagtagcat tcagaaacga
    20161 cgatcatctc cggtttctgc catgctttga tcagacgatt agtatcctgg tgatgggtaa
    20221 agttaccgcc acccgcccac cagatcattt tgatattcgg atacgtttgt tctttaccgt
    20281 tatgctgata tttgccacca ggattttcca gcgcatcgac gatacgggca acacgaatcg
    20341 ccgtcattcc gccatcatca gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt
    20401 ccggcagcac gccgccaaca cgcgtcggat tgccaccgtt ggagtaatga taagagaaac
    20461 caaaaccgcc gcccggtgta ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc
    20521 agtgtttctg ttcaccatat tgctggcgct gaattcccca gccagccatc agcattgtgc
    20581 gattcgccgc catcagttca gcgagtttga ctatctgcgc ttccggtacg ccagtaattt
    20641 ccgccgccca ggccgcgctc ttcggagtat tatcgctctt acctgtcaga tactcttcaa
    20701 actgcggata cccggtggtg tatttttcaa ggaacacttt atcgtgttta ccttgtgtca
    20761 tcagggtatg cgcaatccct aacatcagtg ccacgtcggt gcccatattc ggcgcgatcc
    20821 aggtggcatt atcaccaaag aactcgatgg tttcggagcg gataggatca atggcaatca
    20881 ctggtttgcc agattttttc agctgatgga agtattccag cccttgctca tcggtactgc
    20941 tccaggcaat ttttaaggta ttcagcgggt tcatccccca cagcaccaca gcctggctgt
    21001 tttccagaat cagcggccag gaggtctgct gttcatacac ctctacagaa ccgaccacat
    21061 gcggcatgat cacctgtgct gccccggttg aataatcgcc gctatgcccg gaataaccgc
    21121 ccgccaggtt catataacgt tgcagtaagg tttgcgcttt atgcaacacg ccagaagagc
    21181 gccagccgta tgatccggca aaaatagcag atggtccgtt agctttgcga atgcgatcgt
    21241 gttgctcatg aatcagtttt aatgcttgct cccagctcac ctgtacataa gtatcttcgc
    21301 cacgaccttt cgccggttgc agtggattat cgagatagct ttttcttacc atcggatgct
    21361 gaatacgtgc cgtggtgtgt acctgatccg ccgccgtaga ctgtaaggag ttcggtatgg
    21421 ttttcgccag cgcgcctgtt gaagaaacaa tcttgccgtc cttcacttct acgttcatcg
    21481 ctccccaacg tcccgcggtg aggattttac cgcccttctc ttctgcccat gcggggagcg
    21541 gtgctgccga tgtcaccacc agcgctccag cggcaatacc gctgtgttta ataaattcac
    21601 gtcttgttaa tgtcataact tcctccctga tcaacgagga tcactgtttc tcggtaatat
    21661 ctttggcgtt gtactggaaa taccgcgtta aaatgtccag ttcgttttcg ctcatgctgg
    21721 ttcgcgcacc cattcctttg gcaatggacg gccatgcatt gacggtgtaa tggtcagcgg
    21781 caataggcgc atggcaacca gcgcaatagg tatcgtcaag tttttcagcg tattgccata
    21841 gcggtttacg gtccgctaat gcgggatcgg taagcgcacc ctgtaaagac gcctgacgcc
    21901 attgattgcc gtattcgtca gcctgccatt ccccgtttac cgtgagcgcc ttgatacctt
    21961 cttcacttaa tgtggctagc gccagccgtt gacctgccgc caggtagagc gtgttttcac
    22021 tgccctgcat ttgataaccc tgcaacagaa cgatcggctg tttgccactg gcatcaacga
    22081 cggtgagatc ggtaccagga ttcacggtag ccagctcgcc tatgcgagaa gttttgaaag
    22141 gataaatatg tgcgccatta gtcactgaag tagcggcctg actttccagc tcatgcgccg
    22201 cgttgtcatc catttttatt tctggcggaa aatgggcaat gcctttatga caatcgatac
    22261 aggtttcgcc gcccttttgt gctttgttat gcattttctg cgcagattca ctttgcgagg
    22321 caatatccat ggcatcaaaa gaatggcaac tacggcacgt tgcagagtca gttgctttta
    22381 attctttcca tactgtttcg gccatttcct ggcgatgagt ttcgaactta tcgtcactgt
    22441 ctattttgcc gctaacaaat tcatgataaa tatctttaga tgcttttaat ttagcaaata
    22501 aataatccat ccctgacttt ggaatatggc aatctgcaca ttccgcacgt atccctttct
    22561 ggttcgaaaa gtggacagtt ccctgatatt cctcaaaagg tttactcatc gagtggcagg
    22621 aaaggcaaaa tgctgtatcc gacgttttat gtaaggcttt ttgcgccagc aataacccgc
    22681 cgccaccaac cacaaccgct atcagcaaaa acaataaccc aatgcgtttt ttccctcgca
    22741 tagattcctc taaataaatt ccagaacacc gaaatcattg tataattaaa tacacaacga
    22801 attaaccgct tgcataactc ggcacaacac tgcctgaaat aatcgataaa gaatatgatt
    22861 tattacaatg taatcattaa ttgctaagga ataacccagt tgctatttag aatatatatt
    22921 tatcagtttt a
//
LOCUS       DAZFWL020000069        22892 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFWL020000069 DAZFWL020000000
VERSION     DAZFWL020000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.069"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            210..305
                     /locus_tag="ACHKNP_RS14295"
                     /old_locus_tag="pgaptmp_002859"
                     /pseudo
     CDS             210..305
                     /locus_tag="ACHKNP_RS14295"
                     /old_locus_tag="pgaptmp_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            329..4843
                     /locus_tag="ACHKNP_RS14300"
                     /old_locus_tag="pgaptmp_002860"
     CDS             329..4843
                     /locus_tag="ACHKNP_RS14300"
                     /old_locus_tag="pgaptmp_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HGU4704991.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            4844..6493
                     /gene="yfaQ"
                     /locus_tag="ACHKNP_RS14305"
                     /old_locus_tag="pgaptmp_002861"
     CDS             4844..6493
                     /gene="yfaQ"
                     /locus_tag="ACHKNP_RS14305"
                     /old_locus_tag="pgaptmp_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HGU4704992.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            6498..7274
                     /gene="yfaP"
                     /locus_tag="ACHKNP_RS14310"
                     /old_locus_tag="pgaptmp_002862"
     CDS             6498..7274
                     /gene="yfaP"
                     /locus_tag="ACHKNP_RS14310"
                     /old_locus_tag="pgaptmp_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HGU4704993.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            7549..10398
                     /gene="rcsC"
                     /locus_tag="ACHKNP_RS14315"
                     /old_locus_tag="pgaptmp_002863"
     CDS             7549..10398
                     /gene="rcsC"
                     /locus_tag="ACHKNP_RS14315"
                     /old_locus_tag="pgaptmp_002863"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HGU4704994.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(10484..11134)
                     /gene="rcsB"
                     /locus_tag="ACHKNP_RS14320"
                     /old_locus_tag="pgaptmp_002864"
     CDS             complement(10484..11134)
                     /gene="rcsB"
                     /locus_tag="ACHKNP_RS14320"
                     /old_locus_tag="pgaptmp_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HGU4704995.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(11151..13823)
                     /gene="rcsD"
                     /locus_tag="ACHKNP_RS14325"
                     /old_locus_tag="pgaptmp_002865"
     CDS             complement(11151..13823)
                     /gene="rcsD"
                     /locus_tag="ACHKNP_RS14325"
                     /old_locus_tag="pgaptmp_002865"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HGU4704996.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            14562..15653
                     /gene="ompC"
                     /locus_tag="ACHKNP_RS14330"
                     /old_locus_tag="pgaptmp_002866"
     CDS             14562..15653
                     /gene="ompC"
                     /locus_tag="ACHKNP_RS14330"
                     /old_locus_tag="pgaptmp_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU4704997.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDYK
                     INLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            15765..16820
                     /gene="apbE"
                     /locus_tag="ACHKNP_RS14335"
                     /old_locus_tag="pgaptmp_002867"
     CDS             15765..16820
                     /gene="apbE"
                     /locus_tag="ACHKNP_RS14335"
                     /old_locus_tag="pgaptmp_002867"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HGU4704998.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQTAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            16894..17958
                     /gene="ada"
                     /locus_tag="ACHKNP_RS14340"
                     /old_locus_tag="pgaptmp_002868"
     CDS             16894..17958
                     /gene="ada"
                     /locus_tag="ACHKNP_RS14340"
                     /old_locus_tag="pgaptmp_002868"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HGU4704999.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADSEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANACEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            17958..18608
                     /gene="alkB"
                     /locus_tag="ACHKNP_RS14345"
                     /old_locus_tag="pgaptmp_002869"
     CDS             17958..18608
                     /gene="alkB"
                     /locus_tag="ACHKNP_RS14345"
                     /old_locus_tag="pgaptmp_002869"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HGU4705000.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            18684..20327
                     /gene="yojI"
                     /locus_tag="ACHKNP_RS14350"
                     /old_locus_tag="pgaptmp_002870"
     CDS             18684..20327
                     /gene="yojI"
                     /locus_tag="ACHKNP_RS14350"
                     /old_locus_tag="pgaptmp_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HGU4705001.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGQTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            20545..22191
                     /gene="mqo"
                     /locus_tag="ACHKNP_RS14355"
                     /old_locus_tag="pgaptmp_002871"
     CDS             20545..22191
                     /gene="mqo"
                     /locus_tag="ACHKNP_RS14355"
                     /old_locus_tag="pgaptmp_002871"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HGU4705002.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(22340..22849)
                     /gene="eco"
                     /locus_tag="ACHKNP_RS14360"
                     /old_locus_tag="pgaptmp_002872"
     CDS             complement(22340..22849)
                     /gene="eco"
                     /locus_tag="ACHKNP_RS14360"
                     /old_locus_tag="pgaptmp_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HGU4705003.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
BASE COUNT         5535 a         5624 c         6137 g         5596 t
ORIGIN      
        1 gctcttctga ttggctgtat gtttgatgac aaaaaattag cgcaagaaga aaaaattacc
       61 ttgcgctaat gctctgtctc aggtcactaa tcacacctaa ctcattgatt aattgtgact
      121 ctaaatgtag tgttttgtcg cgttttgcag tatgttattt aatctattat atgaataaca
      181 taatgatttt taatgtattt ttgtaattta ctctccttct gacatggaag gacacccgat
      241 ggatacccaa cgattccaat cccaatttca ttggcattta tcgtttaaat tttctggcgc
      301 gatagcagcg tgcttgtccc tgtctcttgt gggaacaggg cttgctaatg ctgatgattc
      361 gcttccttcc agtaactatg cgccgcccgc cgggggaaca ttctttttgc ttgctgacag
      421 cagttttagc agcagtgaag aggcgaaagt gcgactggaa gcgccggggc gtgattatcg
      481 gcgctatcag atggaagagt acggcggcgt ggacgttcgc ctgtatcgta ttcctgaccc
      541 gatggcattt ttgcgccagc agaaaaacct gcatcgcatt gtggtgcaac cgcaatatct
      601 gggcgacggg ctgaacaata cgctgacctg gctgtgggat aactggtacg gcaaatctcg
      661 ccgcgtaatg cagcgtactt tctcttctca gtcacggcag aatgtgactc aggcattacc
      721 cgaattacat ctcggcaatg ccattattaa accttcccgt tatgtacaga acaaccagtt
      781 ttccccgctg aaaaaatatc ccttggtgga acagttccgt tatccactat ggcaggctaa
      841 accggtcgag ccgcagcaag gggtaaaact ggaaggcgca tccagcaatt tcatctcgcc
      901 gcagccgggt aacatttata ttcctctcgg ccaacaagag ccgggactgt acctcgtcga
      961 ggcgatggtt ggtgggtatc gggcgacgac ggtggtgttt gtttccgata ccgtggcgct
     1021 tagcaaagtg tcaggcaacg agcttctggt gtggaccgcg ggtaaaaaac agggtgaagc
     1081 gaagcccggc tcagagatct tgtggactga cggtcttggc gtgatgaccc gcggtgtgac
     1141 cgatgacagc ggtaccttgc agttacaaca tatatcgcca gaacgttcat acattctggg
     1201 taaggatgct gaaggcggtg tttttgtctc cgagaacttc ttctacgaaa gcgaaatcta
     1261 caacacccgc ttgtatattt ttaccgatcg cccgctatat cgcgcaggcg atcgtgtcga
     1321 tgttaaagtg atgggccgcg agttccacga tccgttgcat tcatccccca tcgtcagcgc
     1381 cccggcgaag ctttcggtgc tggacgctaa cggcagtctg ttgcaaaccg tcaatgtcac
     1441 gctggatgcg cgcaatggcg ggcagggaag tttccgcctg ccagaaaatg ccgtagccgg
     1501 aggttatgag ttacgtcttg cttaccgcaa tcaggtctat agcagcagtt ttcgcgtggc
     1561 aaactacatc aagccacatt tcgagattgg tttagctctc gacaaaaaag agttcaaaac
     1621 tggcgaagcg gtcagcggca aactgcaact gctttacccg gatggtgagc cggtaaaaga
     1681 tgcccgcgtg cagttaagtt tgcgcgctca gcaattatca atggtcggta acgatttgcg
     1741 ttatgccgga cgttttcccg tgtcgctgga aggcagcgaa acggtgtccg acgccagcgg
     1801 tcatgtggcg ttaaatctcc ccgccgccga taaaccgagc cgctatttgt taaccgtctc
     1861 cgccagtgac ggcgcggcgt atcgcgtcac caccaccaaa gagatcctca ttgaacgcgg
     1921 cctggcgcat tactctttaa gtaccgccgc acaatacagt aatagcggcg agtcggttgt
     1981 gttccgttat gccgcgctgg aatcttcaaa acaggttcct gttacgtatg aatggttgcg
     2041 tctcgaagac cgcacgagcc atagcggaga gctaccgtca ggcggcaaat cctttaccgt
     2101 caatttcgct aaacctggca actacaatct gacattacgc gataaagacg gcttaattct
     2161 cgctgggtta agtcatgccg tcagcggtaa gggcagcacg gcgcatactg gtacggtaga
     2221 tatcgtggcg gataaaacgc tgtaccagcc aggcgaaact gcgaagatgc tgattacctt
     2281 tccggagcca attgatgaag cattattgac gctggaacgc gatcgcgtgg aacagcagtc
     2341 gctgctttcg catccggcaa actggctgac gttacaacgt ttaaacgata cccagtatga
     2401 agcccgggtt ccagtgagca attcctttgc gcctaacatc actttttcgg tgctgtatac
     2461 ccgtaatggt cagtacagtt ttcagaacgc cgggatcaaa gttgccgttc ctcagcttga
     2521 tatccgggtg aaaacggaca aaacccatta ccagcctggt gaactggtca atgtcgaatt
     2581 aacctcgtcg ctgaaaggta aacctgtttc tgcgcagcta acggtaggcg tggtcgatga
     2641 aatgatctac gcgctgcaac cagaaatcgc gccgaatatc ggcaaatttt tctatccgct
     2701 ggggcgtaac aatgtgcgta ccagctccag tctgtcgttt atcagctacg accaggcgct
     2761 ctccagcgag ccggttgcgc ctggcgcaac taaccgcagc gagcggcgag taaaaatgct
     2821 tgaacgtcca cggcgtgaag aggtggatac cgcggcatgg atgccgtcac tcacaaccga
     2881 taaacaaggc aaagcgtatt tcacgttcct gatgcctgat tcgttaaccc gctggcgtat
     2941 caccgcgcgt gggatgaacg gcgacgggct ggtcgggcag gggcgtgctt atctgcgttc
     3001 ggaaaaaaat ctctacatga agtggagtat gccaacggtg tatcgcgtgg gcgacaaacc
     3061 ggcggcagga ctgtttatct tcagtcagca ggataacgaa ccggtggcgc tggtgactaa
     3121 atttgcaggc gctgagatgc gccagacgct gacgctgcac aaaggggcga attatatttc
     3181 gctgacgcag aacattcagc aatctggctt gttaagtgca gaactgcaac aaaatgggca
     3241 agtgcaggac agcattagca caaaactgtc ttttgtggac aacagctggc ccgttgaaca
     3301 gcagaaaaat gtcatgctcg gcggtggcga gaacgcgctg atgttgcccg agcaggcgag
     3361 taatatccgg ctacaaagta gtgaaacgcc gcaggagatt ttccgcaaca atcttgatgc
     3421 gttagtcgat ggaccatggg gtggcgtgat caacaccggt agccgtctga tcccgctcag
     3481 tctcgcctgg cgttcgcttg ccgatcatca aagtgccgcc gctaacgaca ttcgtcagat
     3541 gattcaggat aaccgtctgc ggctgatgca actggcgggg cccggagcgc gctttacctg
     3601 gtggggtgaa gatggcaatg gtgacgcctt ccttacggca tgggcatggt acgccgactg
     3661 gcaggccagc caggcgctcg gcgtaacgca acaaccggaa tactggcagc atatgctcga
     3721 cagttatgcc gagcaggcgg ataacatgcc gttattgcat cgggcgctgg tgctggcgtg
     3781 ggcacaggag atgaatctgc cgtgcaaaac gttgttgaaa gggttggatg aagctatcgc
     3841 ccggcgcgga actaaagatg aagatttctc tgaggaagac atccgcgata tcaatgacag
     3901 cctgatcctc gatacaccgg aatctccact ggcagatgcg gtggcaaacg tcttaaccat
     3961 gacgttgctg aaaaaagcgc agttgaagtc cacggtgatg ccacaggttc agcaatatgc
     4021 gtgggataaa gcggcaaaca gcaatcagcc gctggcgcac acggttgtgc tgctcaatag
     4081 cggtggtgac gctacccaga cggccgctat tttaagtggt ttgaccgctg agcaatccac
     4141 tattgagcgc gcgctggcca tgaactggct ggcgaaatat atggcgacaa tgcctccagt
     4201 tgttttgcct gcgcctgcgg gcgcatgggc taaacataag ttaactggag ggggcgaaga
     4261 ctggcgttgg gttggtcagg gcgtgccgga cattctctct tttggtgacg aattatcgcc
     4321 gcaaaatgtg caggtccgct ggcgtgagcc ggcaaaaatg gctcaacaaa gtaacattcc
     4381 ggtgaccgtt gaacgccagt tgtatcggct tatccctggt gaagaagaga tgagctttat
     4441 tctgcaaccg gtgaccagca atgagattga cagcgatgcg ctgtatctcg atgaaattac
     4501 gcttaccagc gagcaggatg cagttctgcg ctatggtcag gtggaagtac cgctcccgcc
     4561 gggagccgac gttgagcgca caacatgggg catttcagtc aataaaccca acgccgcgaa
     4621 acagcagggg caattgctgg aaaaagcgcg taatgaaatg ggcgaactgg cctatatggt
     4681 gccggtgaaa gaactgacgg gaacggtcac tttccgccat ttgctgcgct tctcgcaaaa
     4741 agggcaattc gttctgcctc ctgctcgtta tgtgcgttcc tatgcacctg cgcagcaaag
     4801 tgttgcggca gggagtgaat ggaccgggat gcaggtgaaa taagtgaact ggcgcagaat
     4861 cgtctggcta ttggcgttgg ttactctgcc aacgcttgcg gaggagacgc cgttacaact
     4921 ggtgctgcgc ggtgcgcagc acgatcaact gtatcagctg tcgtcatcag gcgtgaccaa
     4981 agtctcagca ttacctgact cgctcaccac gccgttaggg agtttgtgga aactgtatgt
     5041 ttacgcctgg ctggaggata cccatcaacc ggagcagccc taccagtgtc gcggaaactc
     5101 cccggaagag gtttattgtt gtcaggctgg ggagagcata acgcgcgata ccgcgctggt
     5161 acgttcctgt gggctctatt ttgcgccgca gcggttgcat attggcgcag atgtgtgggg
     5221 gcaatactgg caacagcgtc aggcacctgc gtggttagcg tctcttacaa cgctgaagcc
     5281 agaaacctcg gtaacggtta aatcgctgct cgattcatta gcgacgttac ctgcgcaaaa
     5341 caaagctcag gaagtcttac tggatgtggt gctggacgag gcgaaaatcg gtgtcgcatc
     5401 catgctcggt agtcgggtac gggtgaaaac ctggagctgg tttgccgatg ataaacagga
     5461 aatccgccag ggtggttttg ctggctggtt aaccgatggt acaccgctgt gggttactgg
     5521 cagtggcacc agcaaaacgg tactaacccg ttatgcgacg gtgcttaatc gggttttgcc
     5581 agtgccgacg caggttgcca gtgggcaatg tgtagaggtg gagctgtttg cccgctatcc
     5641 gctgaagaaa atcacagcgg aaaagagtac gacggcagta aaacccggcg tacttaatgg
     5701 gcgctatcgt gtgacgttta ctaatggtaa ccacattacg tttgtcagcc acggtgagac
     5761 gaccttactg agcgaaaagg gcaaactgaa attgcagtcg catctcgatc gtgaagagta
     5821 cgttgccaga gttctggatc gcgaggcgaa aagtacgccc cctgaggcag cgaaagccat
     5881 gaccgtggcg attcgcactt ttttacagca aaacgccaat cgggaaggcg attgcctgac
     5941 catccccgac agcagcgcca cgcagcgtgt ttctgcttcg ccagcgacca cgggggcgcg
     6001 aacgatgacc gcctggacgc aggatctcat ctatgcaggc gatccggttc attatcatgg
     6061 cagtcgagct accgaaggga cactttcctg gcggcaagcc acggcccagg cggggcaggg
     6121 agagcgttac gaccagatac tcgcgtttgc ttatcccgac aacagcctta gccgctgggg
     6181 agcgccacgc tcaacctgcc agttattacc caaagcgaaa gcctggctgg cgaaaaaaat
     6241 gccgcagtgg cggcgtatat tacaaggcga gacggggtac aacgaaccag acgtgtttgc
     6301 ggtctgtcgt ttagtctcag gtttccccta taccgatcgg cagcagaaac ggctgtttat
     6361 tcgtaacttc tttacgcttc aggatcggct ggatttaacc catgagtatc tgcacctggc
     6421 ctttgatggt tatcccaccg ggcttgatga gaactatatc gaaacgctga cccgacaatt
     6481 attaatggac taatgctatg cgaaagattt ttcttccgtt gttactggtg gcactttcgc
     6541 ctgtcgctca cagtgaaggt gtgcaggaag tcgagattga cgcaccgctc tctggctggc
     6601 atccggcaga gggcgaagat gccagttttt cacaatcgat caattacccg gcgtcgtccg
     6661 tcaacatggc agatgatcaa aatatctcag cgcagatccg cggcaaaata aagaattatg
     6721 ctgcggcggg taaagttcag cagggtcggc tggtggtcaa cggtgccagt atgccgcaga
     6781 gaattgaatc cgatggttca tttgcacgcc cttatatttt cactgaaggc agcaacagcg
     6841 tgcaggtcat cagcccggat gggcaaagcc ggcaaaaaat gcagttttac tcaacgccgg
     6901 gtacaggaac gattcgtgca cgtttacggc tggttctctc gtgggatacg gacaataccg
     6961 atctcgatct ccatgttgtc acgcccgatg gcgaacacgc ctggtacggt aacaccgtgc
     7021 tgaaaaacag tggtgcactg gatatggatg tcacgacggg gtacggaccc gagattttcg
     7081 ccatgccagc accagttcac gggcgctatc aggtgtatat caactactat ggtgggcgca
     7141 gcgaaacgga attgacgacc gcccaactga cgctgatcac cgatgaagga tcggtcaatg
     7201 agaaacagga aacatttatt gtaccgatgc gtaatgctgg cgagctgacg ctggtgaaaa
     7261 gttttgactg gtgatccatc ccttcacaca acctggctct ctcttttttc agataagact
     7321 gctgggcgat ctgccgacgc caggatataa ccattaacaa gcgtcggcag attggtcatt
     7381 tcttattttt cgtactgaga actgtccatc tactccggct ttcctggtta aggcaatggt
     7441 ttctaagcga tacgcattat tgcaatggtg acagaacatt gcggtaacac gcttttaccg
     7501 ctaccttaac cacactccat cggtcacctg aggcggagct tcgccccttt gaaatacctt
     7561 gcttcttttc gtacaaccct gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg
     7621 ctctggctgt tgattgcttt ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga
     7681 gaatcggaaa ttcgtcagga atttaatctg agttccgatc aggctcagcg ctttattcaa
     7741 cgcacctctg atgtgatgaa agagctgaag tacatcgccg aaaatcgctt atcggcagaa
     7801 aacggtgtgc tttccccgcg tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg
     7861 ctgtttgccg actccgattg ttccgcaatg agtaacacct ggcgaggttc tctggagtca
     7921 ttggcgtggt ttatgcgcta ctggcgcgat aatttttctg cggcttacga tctcaaccgg
     7981 gtatttttaa tcggcagtga taacctctgc atggccaatt tcggtctgcg tgatatgcca
     8041 gtggaacgcg ataccgcgtt gaaagcgttg catgaacgca tcaataaata tcgaaatgca
     8101 ccacaagatg atagcggcag taacctctac tggatcagcg aaggtccgcg ccctggcgtc
     8161 gggtattttt acgcgttgac gccagtttat ctggcgaacc ggttgcaggc gcttttgggt
     8221 gtcgagcaga ccatccggat ggaaaacttt ttcttaccgg gtacgttgcc gatgggggtt
     8281 accattcttg atgaaaatgg tcataccctg atttcgctta ccggaccaga aagtaaaatt
     8341 aagggcgatc ctcgctggat gcaggaacgc tcctggtttg gctatacgga agggttccgg
     8401 gagctggtgc tgaagaaaaa tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg
     8461 gttgataagg tgctggaacg cattcgcatg ttgatcctta acgcaatttt gctgaatgtg
     8521 cttgccggag ctgcattgtt tactctcgca cggatgtacg agcgacgtat tttcattccg
     8581 gcggaaagcg acgccctgcg actggaagaa catgagcagt tcaatcgcaa gattgtcgcc
     8641 tccgcgccag tgggtatctg cattttgcgt accgctgatg gcgtcaatat tttaagtaac
     8701 gaactggcgc atacctatct caatatgctt acgcatgagg accgccaacg actgacgcaa
     8761 attatctgtg ggcagcaggt caattttgtt gatgtcctga ccagcaacaa taccaatctg
     8821 caaatcagct tcgtccattc gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg
     8881 gatgtttctt cgcgcgtgaa gatggaagag tcgttgcagg agatggcaca agcagcggaa
     8941 caggcgagcc agtcaaaatc gatgttcctt gccaccgtca gtcatgagct gcgaacgccg
     9001 ctgtatggca ttatcggtaa cctggatctg ttgcaaacca aagagttacc gaaaggcgtc
     9061 gatcggctgg tgacggcaat gaacaactct tccagcctgt tgttgaaaat tatcagcgat
     9121 attctcgatt tctcgaagat tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca
     9181 ccgcgtgaag tgatgaacca catcaccgcc aactatttac cgctggtggt acgcaagcag
     9241 ttaggcttgt actgctttat tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg
     9301 cgtttacagc aggtcatctc caacctgctg agtaacgcca taaaattcac cgataccggc
     9361 tgtatagttt tgcatgttcg cgctgatggc gattatctct ctatccgtgt tcgcgatacc
     9421 ggcgtgggca ttccggcgaa agaagtggtg cgcttgtttg atcccttctt ccaggtcgga
     9481 acgggcgtac agcgtaactt ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg
     9541 atcagcatga tggacggcga tatctcggta gattcagaac cgggaatggg cagccagttt
     9601 accgtgcgta ttccgttgta cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg
     9661 agtggtaaac gctgctggct ggcggtccgc aatgcgtcgc tctgtcagtt cctggaaacc
     9721 agtttgcagc gcagcggcat cgtcgttaca acatacgaag ggcaggaacc gactcccgaa
     9781 gatgtgttaa tcactgacga ggtagtgagt aaaaaatggc agggtagagc ggtagtgacc
     9841 ttctgtcgtc gccatattgg tattccgctg gagaaagcgc caggggagtg ggtacacagt
     9901 gtggctgctc cgcatgagct accggcattg ttggcgcgta tttatttgat cgagatggag
     9961 agcgacgatc ctgctaacgc tctgccgtcg acggacaaag cggtcagcga taacgacgat
    10021 atgatgattc tggtcgtgga tgatcatccg attaaccgac gtctgctggc agatcagttg
    10081 ggatcgttgg gctatcaatg taaaaccgcg aatgatggcg tcgatgcgct taatgtactt
    10141 agcaagaatc atattgatat cgtgcttagc gacgtcaaca tgccaaatat ggatggttac
    10201 cgcttgacgc aacgcattcg tcagttggga ctgacgttgc cggtaatcgg agtaactgct
    10261 aatgcgttgg ctgaagagaa gcagcggtgt ctggagtccg gtatggacag ctgcctgtcg
    10321 aagccggtaa cgctggatgt gataaaacag acgctgacgg tatatgccga gagggtcagg
    10381 aaatcgcggg aatcgtagga cggataaggc gtttacgccg catccggcat tcagtgcaaa
    10441 tgccagataa gacactaacg cgtcttatct ggcctacagg tgattagtct ttatctgccg
    10501 gacttaacgt cacagaagag agataattca gcagggcgat atcgttctcg acacccagct
    10561 tcatcatcgc agatttcttc tggctactga tggttttaat actgcggttc agctttttag
    10621 cgatctcggt caccaggaag ccttccgcaa acaggcgcag aacttcactc tcttttggcg
    10681 agagacgctt gtcaccgtaa ccaccagcac tgattttttc caacaggcga gaaacgcttt
    10741 ccggggtaaa tttcttccct ttctgcagcg cggcgagagc tttcggcaga tcggtcggtg
    10801 caccttgttt cagcacgatc ccttcgatat ccagatccaa taccgcacta agaatcgccg
    10861 ggttgttgtt catggtcaga acaataatcg acaggcttgg gaaatggcgc ttgatgtact
    10921 tgattaaggt aatgccatcg ccgtacttat cgccaggcat ggagagatcg gtaatcaaca
    10981 catgcgcatc cagtttcggc aggttgttga tcagtgctgt agagtcttca aattcgccga
    11041 caacattcac ccactcaatt tgctcaagtg atttgcgaat accgaacaag actatcggat
    11101 ggtcatcggc aataattacg ttcatattgt tcatgtaata ggctaccttg ctacagcaag
    11161 ctcttgacat aactgtcaat gtcgctgatg tatttttcta ttcctggaac atccttctca
    11221 cgaatcagat gttccagcgt ttcacataac tgcttgccgg gtaccagatt tagcatggca
    11281 aatacgcctt taagacgatg agccgtttgg gctaacgcag caaagtcact ggttgctgct
    11341 tcagtataca gcctcttaac atcatccggt actgtgtcta caaagagcgc ataatagccg
    11401 ctggcatgga gttgcgcatt ttcatctccg cccagaggcg attctgtcac ctcttcctgc
    11461 gccagttgca cttcaattaa ttgtaagacc gcttcctgca tagcgttgct catattgaag
    11521 ttgacgcaca attgaccagg cccaatttcc cgtacgccag actcatcatc gcttaaaagc
    11581 aagccagagg cagtaagatt agacggatta tccgttaaaa agatatcata atcttgacta
    11641 attaatcttt catcgggtgt gatacaggtt gcaccccaat tttctaactg gcgagtgaca
    11701 atattccgaa tttctgccga agtaacatcc accattacgc agacatcatc cagtaaacgc
    11761 tcttcttcct cttcaacttc cgggtcagcg gcgagcattt tgatatgcac agagtagcgt
    11821 gtaccaagcc catcccgcgt tttgatgttt aaatgaccgc ccagtttacg cgccagttga
    11881 tcgctcagcc agaatgccag cgggtccgcc ttgccatagc gatcgttttg ggtctggttg
    11941 ataaacggga agtgcaaatt atccatttca tgaatactta cgccttctcc ggtgtccaga
    12001 atgcggaatg tcaggcggtc ttcagaggac tcatcctgat caacctcaag ggtgattttt
    12061 cccaattgcg ttgaggtcac ggcatattgc atcagtagca gcaaaatacg tcgtaaggca
    12121 tcgcgatcgc cgcggcgcat atcgtgtgct ttcagatgat tgttaatcag cagttgcaga
    12181 cctttacgct tgatggcagg caacactgaa ggcacaactt catcaattaa atcctgcacg
    12241 gagaacagca ccgtctcact tttccagcta tcatccgcaa gcatgttcgc taactgtatt
    12301 tcatcaacca gccgcaccag cacatctgcc tgattcgcca gttgtttgct ttccggggcg
    12361 ttgagtttag ccgcgctctc cgccagggac tgtgcaggtt ctttcagcgc atcgccaatg
    12421 tttttcataa aggtcatccg gccctgctgg tttttctcat acagacgctg cgcctgcttg
    12481 agtttcttgt ttaccagcac ttcgcgatcc tgatcgcgaa taatgaaaat ttgtgtgcgc
    12541 ggcgcgacct ggctgcggaa catgcggatc tcatacagct cgttattgat cgtcgcctga
    12601 ataatcccct gatgctgttc cgccatggtg gtgatgtttt gcagattcaa atgcggcagc
    12661 aaatgatcgg caattttgtt acttatgaca gtgcggttcg attcctgatc gtgaaccagc
    12721 aggccgagcg gcagcagtga gactatctct tcattaattg cccgtaaaat gcgcaattcg
    12781 ttattgaccg ccgtgctggg tagactttct gtactgcggc tggagaaatg gcggaatgtg
    12841 gtatagccaa ataacgccag cgccagcaaa ccgatgttca gcagcagtgg cagcagaatg
    12901 ttttgcaacg tatccagcaa taaggtgcca taaggaacct gccagaccaa gcgcatatcg
    12961 gtagagttga gtgccgagga gatttcaatc ttcgtactgt taaagtggat actgacgcta
    13021 tccgtccctt ctttctcatt atcattgttt cccgttgccg tcgcgtctgg ctcaaggcgg
    13081 aaactgtcca gcggcatacc cggtgggatc aaatcattaa tcggcagatc aaaagccacg
    13141 accgttgcca gatgtcctgg ctggttaaag gtagtacgca aggtaaagta atgaccgttc
    13201 tgccaggcca ggcggcgcat gttagaaaag ctttcgcgtt catcgagggc gttggcctgt
    13261 tgcaacatct ctgcacgacg tgaatcaaca atgtcgctga cggtcgattc tttaaatccg
    13321 gaggtgagat ctttaagggg tagggttgag atcagcacca gactgttatc ctgaccattc
    13381 aggtaataca tcgaccacgg tacattttct gcgccccaca atgtatccag ataagtggac
    13441 atccgctgag tcatctcaag cgttgagttg tcgtgagagc caaagattaa cgcttccgtt
    13501 ttgcggcgtg gtttctccag atagtagaca tcctgtttca ggcgcgtctc ttgtaaacct
    13561 tcgccggagg agggggaggt cgtcgcggca atgttgtcgt agatctgcca ggtcacgtaa
    13621 cgccaggtat cgacgcgctt ttggatagcg tgggtaatgt cgacaatctg gtaactttta
    13681 tctttcagcc atgcgttaac ggcgctttgt accattacac ccatcgtcac cagtaacaca
    13741 atgatcaaca gtaaaaagaa gcgggtaatg ctccccggta ggagtgaaaa gcgggtcgtg
    13801 gccgttgtct ctttctgacg cattcgtgtt tatgacctgt taaaacttcg cgaagtgata
    13861 gggcagtata aagggtacag tgtgaaattc cagactctta cctgattgat gattctctat
    13921 tctcccgtga tatttatcat cctgcattgg gggaaataga tgtacaagcg ccattttgct
    13981 gtacaaataa cctacaaaaa gcccaacgaa aataattgca aataaacaaa gattgctgga
    14041 aattatgctg atgttaatta tttgtgaaat agttaacaag cgttatagtt tttctgtggt
    14101 agcaaagaat aatgaaaagt gtgtaaagaa gggtaaaaaa aaccgaatgc gaggcatccg
    14161 gttgaaatag gggtaaacag acattcagaa atgaatgacg gtaataaata aagttaatga
    14221 tgatagcggg agttattcta gttgcgagtg aaggttttgt tttgacattc agtgctgtca
    14281 aatacttaag aataagttat tgattttaac cttgaattat tattgcttga tgttaggtgc
    14341 ttatttcgcc attccgcaat aatcttaaaa agttcccttg catttacatt ttgaaacatc
    14401 tatagcgata aatgatacat cttaaaagtt ttagtatcat attcgtgttg gattattctg
    14461 catttttggg gagaatggac ttgccgactg attaatgagg gttaatcagt atgcagtggc
    14521 ataaaaaagc aaataaaggc atataacaga gggttaataa catgaaagtt aaagtactgt
    14581 ccctcctggt cccagctctg ctggtagcag gcgcagcaaa cgctgctgaa gtttacaaca
    14641 aagacggcaa caaattagat ctgtacggta aagtagacgg cctgcactat ttctctgaca
    14701 acaaagatgt agatggcgac cagacctaca tgcgtcttgg cttcaaaggt gaaactcagg
    14761 ttactgacca gctgaccggt tacggccagt gggaatatca gatccagggc aacagcgctg
    14821 aaaacgaaaa caactcctgg acccgtgtgg cattcgcagg tctgaaattc caggatgtag
    14881 gttctttcga ctacggtcgt aactacggcg ttgtttacga cgtaacttcc tggaccgacg
    14941 tactgccaga attcggtggc gacacttacg gttctgacaa cttcatgcag cagcgtggta
    15001 acggtttcgc gacctaccgt aacactgact tcttcggtct ggttgacggc ctgaactttg
    15061 ctgttcagta ccagggcaaa aacggtagcg taagcggcga aggcatgact aacaacggtc
    15121 gtgaagcact gcgtcagaac ggcgacggcg ttggcggatc tatcacttat gattacgaag
    15181 gcttcggtat cggtgctgca gtttccagct ccaaacgtac tgatgatcag aacagcccgc
    15241 tgtacatcgg taacggcgac cgtgctgaaa cctacactgg tggtctgaaa tacgacgcta
    15301 acaacatcta cctggctgct cagtacaccc agacctacaa cgcaactcgc gtaggttccc
    15361 tgggttgggc gaacaaagca cagaacttcg aagctgttgc tcagtaccag ttcgactttg
    15421 gtctgcgtcc gtccctggct tacctgcagt ctaaaggtaa aaacctgggt cgtggctacg
    15481 acgacgaaga tatcctgaaa tatgttgatg ttggtgcgac ctactacttc aacaaaaaca
    15541 tgtccaccta tgttgactac aaaatcaacc tgctggacga caaccagttc actcgtgacg
    15601 ctggcatcaa cactgataac atcgtagctc tgggtctggt ttaccagttc taatctcgat
    15661 tgatatcgaa caaagggcct gcgggccctt ttttcattgt tttcagcgta caaacacagt
    15721 tttttggtgt actcttgcga ccgttcgcat gaggataatc acgtatggaa ataagcttta
    15781 cccgcgtggc actgctggct gccgcgctct tctttgttgg ttgcgatcaa aaaccacaaa
    15841 ccgccaaaac ccacgctact gaagttaccg ttcttgaagg caaaactatg ggtaccttct
    15901 ggcgtgccag catcccgggc attgacgcca aacgcagtgc cgaacttaaa gaaaagattc
    15961 agacccagct ggacgccgac gatcagctgc tttcgaccta taaaaaagat tccgcgctga
    16021 tgcgctttaa cgattcgcaa agtttatcgc cgtggccggt aagtgaagcg atggccgata
    16081 tcgtcaccac ctcgctgcgc attggcgcaa ggaccgatgg tgcgatggat ataaccgtcg
    16141 ggccgctggt gaatctgtgg ggctttggcc cggaacaaca gccggttcaa attccgagcc
    16201 aggaacagat cgatgcgatg aaagccaaaa ccggcttaca gcacctgacg gtcattaatc
    16261 agtcgcatca gcaatatctg caaaaagacc taccggattt atatgtcgat ctctccaccg
    16321 tcggtgaagg ttatgcggcg gatcacctgg cacgcttgat ggagcaggaa gggatttccc
    16381 gctatctggt gtcggtgggc ggcgcgctga acagccgtgg tatgaacggt gaaggcctgc
    16441 cgtggcgggt agcgattcaa aaaccaaccg ataaagaaaa cgcggttcag gctgtggtgg
    16501 atatcaacgg tcatggtatt agcacctctg gcagttaccg taactattac gaactggacg
    16561 gcaaacgtct ttctcatgtt atcgatccgc aaaccgggcg tcccatcgaa cacaatctgg
    16621 tatccgtgac ggtgattgct ccgacggcgc tggaagccga tgcctgggat actggcttga
    16681 tggtactcgg gccggagaaa gccaaagagg ttgttcgccg ggaagggctg gcggtttata
    16741 tgatcaccaa agaaggcgat agctttaaaa cctggatgtc accacagttt aaaagcttcc
    16801 ttgtcagcga aaaaaattaa agcgcaagat tgctggtttt tgcgtgatgg tgaccgggca
    16861 gactaaaggc tatccttaac cagggagctg attatgaaaa acgccacatg cttaactgac
    16921 gatcaacgct ggcaatctgt cttagcccgc gacccgaatg ccgacagcga attcgttttc
    16981 gccgtgcgca ccacaggcat cttttgccgt ccgtcttgcc gcgccagaca tgccttgcgg
    17041 gaaaatgtct ccttctacgc aaatgcctgc gaggcactcg ccgccggatt tcgcccctgc
    17101 aaacgctgcc aacctgataa agccaatgcc cagcaacatc ggttggataa aatcacccac
    17161 gcgtgtcgac tgctggaaca ggaaacgcct gtaacgctgg aagccttagc cgaccaggtg
    17221 gcgatgagtc catttcatct acatcggttg tttaaagcga ctaccggaat gacgcctaaa
    17281 gcctggcaac aggcctggcg cgctcgccgt ttgcgcgaat cgctggcgaa aggggagagc
    17341 gtgacgacgt ctattcttaa cgccggattc cccgacagca gcagttacta tcgcaaagct
    17401 gacgaaacgc tgggcatgac ggctaaacaa ttccgtcacg gtggcgaaaa tctggcggtg
    17461 cgttacgcgc tggctgattg tgagctgggt cgttgcctgg tggcagaaag cgagcggggg
    17521 atttgcgcga tattgctggg cgatgatgac gcgacactaa tcagcgagtt gcagcagatg
    17581 tttcccgctg ccgacaacgc gcctgccgat ctgatgtttc agcaacatgt gcgtgaagtg
    17641 atcgccagcc tcaatcaacg cgatacgccg ctgacgttac cgctggacat tcgcggcact
    17701 gcttttcagc aacaagtctg gcaggcactg cgcacgatac cttgcggtga aaccgtcagt
    17761 tatcagcaac tggctaacgc catcggcaaa ccgaaagcgg tacgggccgt tgccagcgcc
    17821 tgtgccgcca acaagctggc tatcataata ccctgtcatc gggtggtccg tggtgatggc
    17881 acactttccg gttaccgctg gggcgtgtcg cgtaaagcgc aactgctgcg ccgcgaagct
    17941 gaaaatgagg agaggtaatg ttggatctgt ttgccgatgc tgaaccgtgg caagagccac
    18001 tggcggctgg agcggtaatt ttgcaccgtt ttgcttttaa cgctgcggag caactgatcc
    18061 gcgatattaa tgacgttgcc agccagtcgc cgtttcgcca gatggtcacc cccgggggat
    18121 ataccatgtc ggtggcgatg accaactgtg ggcatctggg ctggacgacc catcggcaag
    18181 gttatcttta ttcgcccatt gatccgcaaa caaataaacc gtggcccgcc atgccacaaa
    18241 gctttcatga tttatgtcaa cgtgcggcta cggcggcggg ctatccggat ttccagccag
    18301 atgcttgtct tatcaaccgc tacgctcctg gcgcgaaact gtcgctgcat caggataaag
    18361 acgaaccgga tctgcgcgcg ccgattgttt ctgtttctct tggcttaccc gcgatttttc
    18421 aatttggtgg cctgaaacgt aatgatccgc tcaaacgttt gttgttggaa catggcgatg
    18481 tggtggtatg gggcggtgaa tcgcggctgt tttatcacgg cattcaaccg ctgaaagcgg
    18541 ggtttcatcc actcaccggc gactgccgct acaacctgac attccgtcag gcaggtaaaa
    18601 aagaataaaa ataagaatta ttattgctgt gcgcgcgaag atgtttaaac tgcgggctgt
    18661 aattcattgt ccgggttttc tgcatggaac ttcttgtact tgtctggcgg cagtatcgct
    18721 ggccatttat cagtgtgatg gcgctaagcc tcgccagtgc ggcattaggc attggcttaa
    18781 ttgcttttat caatcagcgc cttatcgaaa cggcggatac cagtctgctg gtattgccgg
    18841 agtttctggg attattgctg ctgttgatgg cagtcactct cggatcgcaa ctggcgctca
    18901 ccactttggg gcatcacttc gtttaccgac tgcgtagtga atttatcaag cggattctgg
    18961 ataccaacgt cgagcgcatt gaacaactcg gtagcgcctc gttgctggcg ggattaacca
    19021 gcgatgtgcg caatatcacc attgcttttg tgcgtcttcc ggaactggtg caggggatca
    19081 ttctcactat cggttccgcg gcgtatctgt ggatgctgtc gggcaaaatg ttgctggtaa
    19141 cggctatctg gatggcgatc accatctggg gtggttttgt actggtggcg cgggtgtaca
    19201 aacatatggc gaccctgcgt gaaaccgaag acaggctgta tacggatttt caaactgtac
    19261 tcgaagggcg caaagagctg actctgaacc gggaacgcgc cgagtatgtg tttaacaacc
    19321 tctacattcc tgatgcgcaa gagtatcgcc accatattat ccgcgcagac accttccatc
    19381 ttagcgccgt gaactggtca aacatcatga tgttgggcgc aatcggcctg gtgttctgga
    19441 tggcgaacag tctcggttgg gctgatacca acgttgccgc gacctattcg ttgacgcttt
    19501 tattcctgcg tacgccgctg ctttcggcgg ttggcgcatt gccgacgctg ctgacggcgc
    19561 aggtggcgtt taacaagctg aacaaattcg cgctcgcgcc tttcaaagca gagtttccgc
    19621 gcccgcaggc gtttcccaac tggcaaacgc tggagctgcg taacgtgacg tttgcttatc
    19681 aggataacgc gttttccgtt ggtccgatta atctcaccat caaacgtggc gagctgctgt
    19741 ttctgattgg cggcaacggt agcggaaaat cgacgctggc gatgttgttg acgggcttgt
    19801 atcagccaca aagcggcgaa atcttgctgg atggcaaacc tgtcagcggc gaacaaccgg
    19861 aagattatcg caaactgttt tcggcagtgt ttaccgatgt ctggctgttt gatcaactgc
    19921 tggggccgga gggtaaaccc gctaacccgc aactggttga gaagtggctg gcgcagctga
    19981 aaatggctca taagcttgag ttaagcaacg ggcgtattgt taacctgaag ttatcaaaag
    20041 ggcagaaaaa acgcgtggcg ctgttgctgg cgctggcaga agaacgcgat attatcctgc
    20101 tggatgaatg ggcggcggat caggatccac acttccgtcg tgagttttat caggtgttgc
    20161 tgccgctgat gcaggagatg ggtcaaacta ttttcgccat cagtcatgat gatcattact
    20221 ttatccacgc cgaccgcctg ctggaaatgc gcaacgggca acttagcgag ctgacgggcg
    20281 aagagcgcga tgccgcttcg cgtgatgccg ttgcccggac ggcataatat tacgacgctg
    20341 acctggcgga ctgctgccgt caggtcaata tgctaaaaat cccttcattt ctaataaccc
    20401 tataattaat caacgaaatt ataatgtttc taaaattaga atataattta taaacattat
    20461 ttaaatgttg ttacttaagt gttaccgttg atgccgcgca aatctcacct gcaataaagc
    20521 gactaaaagt aaggcattaa caagatgaaa aaagtgactg ccatgctctt ctcgatggcc
    20581 gtggggctta atgccgtttc gatggcggca aaagcgaaag cgtccgagga gcaggaaact
    20641 gatgtactgt tgattggcgg cggcattatg agcgccacgt tggggaccta tttacgcgag
    20701 ctggagcctg aatggtcgat gaccatggtg gagcgcctgg agggtgtcgc gcaggagagt
    20761 tcgaacggct ggaataacgc cggaaccggg cattctgcac tgatggaact gaactacacc
    20821 ccgcaaaacg ccgatggcag catcagtatt gaaaaagcag tcgccattaa cgaagcattt
    20881 cagatttccc gccagttctg ggcgcaccag gttgagcgcg gcgtgctgcg tactccgcgt
    20941 tcatttatca ataccgttcc gcatatgagc tttgtctggg gcgaggataa cgttaatttc
    21001 ctgcgcgccc gttacgccgc gttgcaacaa agctcgctgt ttcgcggtat gcgttactct
    21061 gaagatcacg cgcagatcaa agagtgggca ccgttagtga tggaagggcg cgatccacaa
    21121 cagaaagtag cggcaacacg cacagaaatt ggcactgatg tgaactatgg cgagatcacc
    21181 cgccagttaa ttgcttcctt acagaagaaa tctaacttct cgctgcaact cagcagcgaa
    21241 gtccgcgccc taaagcgtaa tgacgataac acctggaccg ttaccgttgc cgatctgaaa
    21301 aacggcactg cacagaacat ccgcgccaaa tttgtcttta tcggcgcggg cggtgcggcg
    21361 ctgaagctat tacaggaatc ggggattccg gaagcgaaag actacgccgg tttcccggtg
    21421 ggcggacagt tccttgtttc ggaaaacccg gacgtggtta atcaccatct ggcgaaggtt
    21481 tacggtaaag catccgttgg cgcaccaccg atgtcggttc cgcatatcga tacccgcgtt
    21541 ctggacggta aacgcgtagt gctgtttggg ccatttgcca ccttctcaac caaattcctc
    21601 aaaaacggtt cattgtggga tctgatgagt tccaccacca cctctaacgt gatgccgatg
    21661 atgcacgtcg ggctggataa tttcgatctg gtgaaatatc tggtgagtca ggtgatgttg
    21721 agtgaagagg atcgttttga agcgttgaaa gagtactatc cgcaggcaaa aaaagaggac
    21781 tggcgtttgt ggcaagcggg gcagcgcgtg cagattatca agcgtgatgc cgagaaaggt
    21841 ggcgtactgc gtctgggtac tgaagtggtc agtgaccagc aagggaccat tgccgcgctg
    21901 ctgggggcat cgccaggggc gtcaaccgcc gcgccgatta tgttggatct gctggaaaaa
    21961 gtatttggcg atcgcgtttc cagcccgcaa tggcaggcta cgttgaaagc gatcgttccg
    22021 tcttatggac gcaagctgaa cggtgatgta gcggcaacag aacgcgagtt gcagtacacc
    22081 agcgaagtgc tggggctgaa atacgacaag ccgcaggcag cagatagtac gccaaaaccg
    22141 cagttgaaac cgcaacccgt tcaaaaagaa gtggcggata ttgcgttgta atgatgtgcc
    22201 acatccggca tggtatgccg gatgtggcgt atgctgataa gacgcgccag cgtcgcatca
    22261 ggcaccggct cgggcgttag atgtcggatg cggcgtgacc gccttattcg gcctacgggg
    22321 tgccgcactt cacagcattt tagcgaacta ccgcgttgtc aattttctct tccgccttcc
    22381 agacgcggta cttcacatct acattgtctg gcgtatacac cacgatcggc agcttgctgt
    22441 tgtaacgcag cattccagca tcgcccagat acgcggtgac aaatttcttc tctttcttgc
    22501 catccgggca ggccatcatc gtggaaaccg gcgagctaac tttatcaaag acataataat
    22561 catagcccca gccttccagc gttttgcttt ccagcttccc gccgagacga tgcaaattgc
    22621 aatcgacttc cagcgtctga ccgattaaca gttctacttt cagggtagat tcatcttctt
    22681 gcggggttaa ctgaatcacc tgacgcttca tccctttttc agcttgtgga taaggcgcga
    22741 ttttttccag tggctggacg ctttctgccg cccaggcgga agtggtagcg aaagcggcaa
    22801 acaatactgc aggtagaatg gtcttcattt ttttgctttc cttcttcaat ttataggttt
    22861 tcaccgatga tgtcgctgaa ggttaacata tt
//
LOCUS       DAZFWL020000070        22689 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFWL020000070 DAZFWL020000000
VERSION     DAZFWL020000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.070"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            22..158
                     /locus_tag="ACHKNP_RS14365"
                     /old_locus_tag="pgaptmp_002873"
     ncRNA           22..158
                     /ncRNA_class="other"
                     /locus_tag="ACHKNP_RS14365"
                     /old_locus_tag="pgaptmp_002873"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            202..338
                     /locus_tag="ACHKNP_RS14370"
                     /old_locus_tag="pgaptmp_002874"
     ncRNA           202..338
                     /ncRNA_class="other"
                     /locus_tag="ACHKNP_RS14370"
                     /old_locus_tag="pgaptmp_002874"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(429..1115)
                     /locus_tag="ACHKNP_RS14375"
                     /old_locus_tag="pgaptmp_002875"
     CDS             complement(429..1115)
                     /locus_tag="ACHKNP_RS14375"
                     /old_locus_tag="pgaptmp_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HGU4705004.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(1696..2484)
                     /gene="phoH"
                     /locus_tag="ACHKNP_RS14380"
                     /old_locus_tag="pgaptmp_002876"
     CDS             complement(1696..2484)
                     /gene="phoH"
                     /locus_tag="ACHKNP_RS14380"
                     /old_locus_tag="pgaptmp_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HGU4705005.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3103..4374)
                     /gene="efeB"
                     /locus_tag="ACHKNP_RS14385"
                     /old_locus_tag="pgaptmp_002877"
     CDS             complement(3103..4374)
                     /gene="efeB"
                     /locus_tag="ACHKNP_RS14385"
                     /old_locus_tag="pgaptmp_002877"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HGU4705006.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(4380..5507)
                     /gene="efeO"
                     /locus_tag="ACHKNP_RS14390"
                     /old_locus_tag="pgaptmp_002878"
     CDS             complement(4380..5507)
                     /gene="efeO"
                     /locus_tag="ACHKNP_RS14390"
                     /old_locus_tag="pgaptmp_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HGU4705007.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5565..6395)
                     /gene="efeU"
                     /locus_tag="ACHKNP_RS14395"
                     /old_locus_tag="pgaptmp_002879"
     CDS             complement(5565..6395)
                     /gene="efeU"
                     /locus_tag="ACHKNP_RS14395"
                     /old_locus_tag="pgaptmp_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HGU4705008.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(7061..8569)
                     /gene="putP"
                     /locus_tag="ACHKNP_RS14400"
                     /old_locus_tag="pgaptmp_002880"
     CDS             complement(7061..8569)
                     /gene="putP"
                     /locus_tag="ACHKNP_RS14400"
                     /old_locus_tag="pgaptmp_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HGU4705009.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(8728..8937)
                     /locus_tag="ACHKNP_RS14405"
                     /old_locus_tag="pgaptmp_002881"
     CDS             complement(8728..8937)
                     /locus_tag="ACHKNP_RS14405"
                     /old_locus_tag="pgaptmp_002881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705010.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            8992..12954
                     /gene="putA"
                     /locus_tag="ACHKNP_RS14410"
                     /old_locus_tag="pgaptmp_002882"
     CDS             8992..12954
                     /gene="putA"
                     /locus_tag="ACHKNP_RS14410"
                     /old_locus_tag="pgaptmp_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4705011.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPLSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(12994..13632)
                     /gene="rutR"
                     /locus_tag="ACHKNP_RS14415"
                     /old_locus_tag="pgaptmp_002883"
     CDS             complement(12994..13632)
                     /gene="rutR"
                     /locus_tag="ACHKNP_RS14415"
                     /old_locus_tag="pgaptmp_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HGU4705012.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            13920..15011
                     /gene="rutA"
                     /locus_tag="ACHKNP_RS14420"
                     /old_locus_tag="pgaptmp_002884"
     CDS             13920..15011
                     /gene="rutA"
                     /locus_tag="ACHKNP_RS14420"
                     /old_locus_tag="pgaptmp_002884"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HGU4705013.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            15011..15703
                     /gene="rutB"
                     /locus_tag="ACHKNP_RS14425"
                     /old_locus_tag="pgaptmp_002885"
     CDS             15011..15703
                     /gene="rutB"
                     /locus_tag="ACHKNP_RS14425"
                     /old_locus_tag="pgaptmp_002885"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HGU4705014.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            15715..16101
                     /gene="rutC"
                     /locus_tag="ACHKNP_RS14430"
                     /old_locus_tag="pgaptmp_002886"
     CDS             15715..16101
                     /gene="rutC"
                     /locus_tag="ACHKNP_RS14430"
                     /old_locus_tag="pgaptmp_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HGU4705015.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            16109..16909
                     /gene="rutD"
                     /locus_tag="ACHKNP_RS14435"
                     /old_locus_tag="pgaptmp_002887"
     CDS             16109..16909
                     /gene="rutD"
                     /locus_tag="ACHKNP_RS14435"
                     /old_locus_tag="pgaptmp_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HGU4705016.1"
                     /translation="MKLSLSPPPYADTPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            16919..17509
                     /gene="rutE"
                     /locus_tag="ACHKNP_RS14440"
                     /old_locus_tag="pgaptmp_002888"
     CDS             16919..17509
                     /gene="rutE"
                     /locus_tag="ACHKNP_RS14440"
                     /old_locus_tag="pgaptmp_002888"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HGU4705017.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            17520..18014
                     /gene="rutF"
                     /locus_tag="ACHKNP_RS14445"
                     /old_locus_tag="pgaptmp_002889"
     CDS             17520..18014
                     /gene="rutF"
                     /locus_tag="ACHKNP_RS14445"
                     /old_locus_tag="pgaptmp_002889"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HGU4705018.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            18035..19363
                     /gene="rutG"
                     /locus_tag="ACHKNP_RS14450"
                     /old_locus_tag="pgaptmp_002890"
     CDS             18035..19363
                     /gene="rutG"
                     /locus_tag="ACHKNP_RS14450"
                     /old_locus_tag="pgaptmp_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HGU4705019.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(19446..19619)
                     /gene="ymdF"
                     /locus_tag="ACHKNP_RS14455"
                     /old_locus_tag="pgaptmp_002891"
     CDS             complement(19446..19619)
                     /gene="ymdF"
                     /locus_tag="ACHKNP_RS14455"
                     /old_locus_tag="pgaptmp_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGU4705020.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            19992..20588
                     /gene="wrbA"
                     /locus_tag="ACHKNP_RS14460"
                     /old_locus_tag="pgaptmp_002892"
     CDS             19992..20588
                     /gene="wrbA"
                     /locus_tag="ACHKNP_RS14460"
                     /old_locus_tag="pgaptmp_002892"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU4705021.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            20609..20836
                     /gene="yccJ"
                     /locus_tag="ACHKNP_RS14465"
                     /old_locus_tag="pgaptmp_002893"
     CDS             20609..20836
                     /gene="yccJ"
                     /locus_tag="ACHKNP_RS14465"
                     /old_locus_tag="pgaptmp_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HGU4705022.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(20874..22115)
                     /gene="agp"
                     /locus_tag="ACHKNP_RS14470"
                     /old_locus_tag="pgaptmp_002894"
     CDS             complement(20874..22115)
                     /gene="agp"
                     /locus_tag="ACHKNP_RS14470"
                     /old_locus_tag="pgaptmp_002894"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HGU4705023.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(22407..>22689)
                     /locus_tag="ACHKNP_RS14475"
                     /old_locus_tag="pgaptmp_002895"
     CDS             complement(22407..>22689)
                     /locus_tag="ACHKNP_RS14475"
                     /old_locus_tag="pgaptmp_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705024.1"
                     /translation="DTDPEQKVNEVKNFLTALSDSICSLFIVNGNVASISLGNESFST
                     DDDLEYGYLINTGNHYDVYLPPELFAQAYELNNKERNAQIDFLTRYAIY"
BASE COUNT         5411 a         6069 c         5814 g         5395 t
ORIGIN      
        1 aagtacgggc ttttttttcg tatatttcac acaccggggg gatgagaagc cccgaccggg
       61 gttcgacaac tggcaacagc cagttggaca gaccgtgaac gcagtgagcg ggctgcccgc
      121 agggcgagcg aagcgagtca atccccccct caccgccata tttaaagaag agctcgtacg
      181 aaagtacggg ctttttttcg tatattgcac acaccggggg gatgagaagc cccgaccggg
      241 gttcgacaac tggcgacagc cagttggaca gaccgtgaac gcagtgagcg ggctgcccgc
      301 agggcgagcg aagcgagtca atccccccct caccgccatc tttaaagaag agctcgtacg
      361 aaggtacggg ctttttttcg tatattgcac acaccggggg gatgagaagc cccgaccggg
      421 atgcttagtt aatgcaccgg ttcactcttt gtcgccgcga ctaatgggcg aattttgtca
      481 cgagtctccc agaccggagg aatttcctcc aatttagtga cagaagctcg tacaaaaggc
      541 acccgtgcaa gagtcataat tgtatccaac caacggtcac gaataatgcg atccgccctt
      601 tcatgactcg ggtcatccag ctcaataaca cagactattt tattgttctt ctttgaaacc
      661 agaacataat ccattgccat gccattataa attgcaaaga aacgctttct ttcttcttcg
      721 ctaaactcag gttgttctgg aaagttgatc aagcgcccca gagagacctg gcaatggata
      781 tgacaataat gtccaaacca tttttccaga ttatcgaaat acattttttc acaacgcgtg
      841 attaaccact cttttctttc caataaaata ccttcggcaa ccattccctg aaataactca
      901 tctcgctctt gccaacgaga gtcacgatag gttttctgcg gtatatagct ccttgtcgac
      961 gccttagcgg caggggaagt tgcagatgaa gggacgggtt gcgatttagc gatgattacc
     1021 gatgaggcaa cttttcggag tgtcaatttt ctggcaacag caaaaccggc gataaatgca
     1081 attgtcaaac aagttacaaa agccataaag ctcataatta attttcacct atccatgttg
     1141 attatatttc attaccaatg ttactcgctg cagcgtgtta catgccaaca atgcaacgat
     1201 caatatatag ttagtatcat gttttgatta ttacaaccat ccctatttag ccattgcggc
     1261 ttgccttcca caaaaaaata acaacacaca atattagcaa agaattaatt aagattaacc
     1321 ttacatttat aatattatcc ctaaagaata aatcagagga atatattcgg tgttaaacag
     1381 tcagtcaaag tatagcccat tgttgattaa acctcataag caaaattggc accagatctc
     1441 ctgtacaggt tgaatggccg aaaaaagata ggcagatgct catttttttg gcttctggaa
     1501 gaaaaactct ccgcgctggc agtattacac tggtctgatg ctatgacgaa aaatgcctgg
     1561 taagagtatc taaaccagac tccagttcag cttatcaaca cgctcaaagt cgcccccctg
     1621 aacgctaacc tgaagtgaaa atctgttgaa ataaaacaaa gcccggttcg cccgggctct
     1681 gcaccgataa tacacttaac tgtaggcatg cagcgtacgt tggcaaagtg ccgaacgtac
     1741 gcagtcctct ttaccgaacc ggacgatccc aaccatttca tcttcttcga aacgttccag
     1801 cgcgtcactt aatccggagc acacgccgcg aggcaaatcg cattgcgtga tatcaccgtt
     1861 gacgataacc gtcacgttct ccccgaggcg ggttaaaaac attttcattt gcgcggcagt
     1921 cacattctgc gcctcgtcaa gaatgacgac tgcattttca aaggtacgtc cacgcatata
     1981 ggcgaacggc gcaatttcca ccttccctat ttccggtcgc aggcagtact gcataaagga
     2041 agcccctaag cgccggacca gcacgtcgta gaccgggcga aaatagggag caaacttttc
     2101 tgcgatatct ccaggtaaga agccaagatc ttcatcagct tgcagaactg gacgggtgac
     2161 gataatcctg tcgacatcct tatgtatcag ggcttctgcc gcttttgctg cgctgatcca
     2221 ggtttttccg cacccggctt cgcccgtggc gaatatcagc tgcttactct caatagcctt
     2281 cagatagtgc aattgcgctt catttcgcgc gaggatgggc gaagtatcgc gactgtcgcg
     2341 ggccatacca atggcttcta cgccgcccat ctgcacaagc gaggtgaccg attcttcttc
     2401 acgctgctta tggctgcgcg aatcccgtct cagcacacgt tttgcctcgc gacgagcttt
     2461 gatcactgct ttttgtcttc ccatggagag caccttgagt tgtttgtatt catcacacgc
     2521 gccgttggca gcgcgattat gcgcacgaac atcagagggt tggcttcctt gtaagccata
     2581 gtttgctttt ggataaaatg ccgaaaaacg gctacgcaca ccgtttgcgg cgtcggtaac
     2641 acgagtaaag agaggaagtg agaaaaatac agagtgacga agttgctacc ggaggagaaa
     2701 tctccgcaag tggtggagcg tagcttaaca agacattgtc cagaacagga cgttaccatc
     2761 cgcgatctcc atagtgactg actatcactg ccgggaactt ccgctgctac ttaataatta
     2821 caacagatct cgcattcatt gcaacaatat attcacttat atttaaccat aaaacaccat
     2881 ttcagtgaca ttagtttcta ctggaaagat gacagagtga tgacagatat gaaaaaagct
     2941 gtgtgctttc agcaggattc gcatcaggca tctgcgcacg aacgccggat gcggcgtgaa
     3001 cgccttatcc gcctaccgaa cccgcaccga ttgtaggcat aagaagacgc gcaagcatcg
     3061 catcaggcat ctgcgcaaga acgccttatc cgtctataaa cattaaaccc gcaataacgc
     3121 gcttccgaga taatcgttcg cgtccttcac tcccggcagc gcaaaaaaat aaccgccgcc
     3181 gataggttta acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa
     3241 gcctttttcc agatcgtgtt ggtaacagac aaacagtagc cccatatcca gttgcccgga
     3301 gttggtgacg cccagtgaat aactgtagcc acgacgcagc atcaggctgg actcactctc
     3361 cgccgtgcgg ggattcgcca gccggatatg gctgtccagc gcaatcacct tcccttccgg
     3421 atcgctggcg taatcgggaa catcatgctc atgctgcatt cccagcggcg caccggtttg
     3481 cttatcacga ccaaaaatcg tctgctgttc tttcagcgga gttctgtccc aaaattccac
     3541 tcgaaactga atcaagcgta ctgcctgata gctgccaccg attgtccacg ctggctcctg
     3601 ctgatctgcc gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt
     3661 ggcagtgccg tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc
     3721 cgcgtgatcg gaaataaacc cttcccgctt ccagcgcacg ctgagcaaat ccggcgtatg
     3781 tttgatgata tcgcgcagtg catggatcac cgtgtcctgg gtgttggcgc aaatttgtag
     3841 caacacatca ccatgacata acgccgcatc cagcgagtcg ttgggaaaac gcgtcatctt
     3901 ctgcaacttt ttcggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc
     3961 caccgataac gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc
     4021 gagtggtggc aggcgcggat ttggcgtttc tggtgccgct ccgccctgag tcagaaaagc
     4081 aaaacgctga gtcaacaagc gaaacaaccg ttcaagatcg gctttatcgc tggcaagcac
     4141 atcaaacgcc accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc
     4201 accataaaac ggctgtttct cattgcgtgc atccggtgaa agcgtacccg gcgcactttg
     4261 cgttttttgt gcatgagcga ccggacaact tcccgccagc gccagcgcac caatgccttt
     4321 cagtaaacgt cggcgtgacg gttcattcac gccgttttta tcttcatact gcataatgct
     4381 taatccaatc ccagcacacc gcgaagttgc gccagatctt ccgccagcgc agtaatcggt
     4441 cctttcagtg cattccggtc ggcatcggtc aatttgtcgt aggtttcaaa accgtcttta
     4501 gtacggtatt tcgccagaat ggtatcgacc tttttaaagt tggcatcgac ttttgccaac
     4561 agttccgggt tggctttttg cagttgtgga cgcagcagat cgacaatttt ctgcgagcct
     4621 tcaacgttag cctggaaatc ccacagatcg gtgtggctgt agcgatcttc ttcaccgctg
     4681 attttgctgg ctgccacttc ctcaatcagt ccggctgcac cgccgaccac ttttgaaggt
     4741 gggaaagcca gttcactgat gcgtttttgc aaatcgacca catcggtata aagctggtca
     4801 gcgtactgat ccatgccttt ggtggtgttg tcgccaaaca atgctttttc cagacggtgg
     4861 aaaccggtga attttggatc agcggctttt tgctcgtaat catcttcacg ggcgtcaatg
     4921 ctgccatcca gatcggagaa cagttcagca atcggttcaa tgcgctcata gtgctggcgc
     4981 gtcggcgcat acagtgcttt cgctttttcg atatcgcctg ctttaatcgc gtcggtaaag
     5041 gctttggtgt cggtcaccag ctgcgtggtt tccgccatga catacgcttt atatgcagta
     5101 attgcaccac caagacttaa cagcgcatca ctttgcgccg catccgccgt tgcctcacct
     5161 ttgacgatca acttcccttt cgggttagtc agcagaccgc aggtcatatc gtattcgcca
     5221 ggctgtaaat tcgccgtcat tttctggcta aaaccagggg cgatattttc ccgctcttcc
     5281 accaccatca cgcctttgag gatctcccac tccagcgcct tctggctatg gttctgaata
     5341 atgaactgtg ttttcccggc gttaaccgta atggtcatcg gttcgcactg cttatccgtc
     5401 acggtcactt tgacctgcgt cacatcagcg gcgttagcca taaaagcaga agaaaacagc
     5461 gcagccacgc tcaactgcaa tgcgttacgg cggaagttaa tggtcatgac gagactatcc
     5521 ctttaaagta tgttgtaact aagcaagagt ttgcgtcgta tttgttacgc ggagcgagac
     5581 gctgtcgccc ctgcgcgtgg tggcagagca aatgccacca gcgccgggat gagataaata
     5641 aaccagacgg cgacttcgct gacgctcggc gcttcctgat agccaaaaat cccttccatc
     5701 agcgtgccaa acagcgagtg agttgagagc accgcactca tatcgaaggc gatttcctga
     5761 aagtggttcc acaatccggc ttcatgaaat gcgcgaatgg caccagctgc cagccctgcg
     5821 gcgacgaaga gaataaacag gctggtccat ttaaaaaatg caccaagatt gaggcgaata
     5881 ccgccccagt agagcaagaa gcctagcacc acggcagtag caagaccgag cattgcaccc
     5941 agcggcggcc agatcccgac atcttgttga aatgccgcca gcaggaaaaa gaccgactcc
     6001 agcccttccc ttgcaacggc aaaaaagacc atcatcacca gcgcccagcc atgatgattt
     6061 ccacgctgca atgcgctatc gactgcctgc tccagttgta ctttgacgtt gcgcgacact
     6121 ttgcgcatcc agaaaaccat ccaggtaagg atcaccacgg cgatcaccgc cacgatacct
     6181 tcaaacagtt cctgctcttt ttgcggaaat tcgccggtgg tttcgttaat gaagatcccc
     6241 aagcccaggc acaacgcagc ggcaagcaac acgccaatcc acatcacacc aatccatcgg
     6301 cctcgctggg tacgcttaag atagctggca atcaaactga caatcagcgc ggcttcaagt
     6361 ccttcgcgca acataatgag aaacggaaca aacatgccac ccatccttaa acgtttttct
     6421 gagtcaatct atgcaaagaa aagtaaatcg cagtgatagc gattatcatt acggtaaaga
     6481 aaaatacaag cggaatgttt ataaaatgat gttgaaatgt aaacaaagag aggtaatgcc
     6541 aggggatata aacgattatt ccaggtacaa agatgggatt gtttacaacg agccatgtgt
     6601 tcttgccgga tgccgcgtaa acgccgtatc cagccgacaa aaccgtcctg actcaataca
     6661 tcaaaacttc catgtaaaac tgataagcgg gtgtatcaag caatttcgtc catcctcgat
     6721 tatcaaatct aactttttgc ccggaacata agaacagcgc gggtgttggt ggcgtcaaaa
     6781 tcgctgaaac gttccccgaa ggccggggca gcccacatgg atgtgggctg agggcgcgtc
     6841 ttacaggacg ttacctcgcg cccgacccgg tagccgaagg ggataagctg aaggtaccac
     6901 gcagcggcga ttttgttcgc cagagcccgg gggtgcaggg ggccgcggcg attggccgcc
     6961 ccctgcgcgc tccctgcacc attcaattta tatggcgagg cacattaagg gagcgcaacc
     7021 cgctgctgaa gccaaacaaa accgccgcag gctaagtccc ttagctttcc tgcaaccgtg
     7081 acggcggagc cgaatgatag tgcgcatcgg cctcggcaaa gcgtttttgc atcgccgctg
     7141 acggcgcttt acccagcaaa ctaaacacta caatcccgat actgccgaag atgaagcccg
     7201 gaataatttc gtagagtccc agccagccga actgcttcca gacgataacc gtcagcgcac
     7261 cgatgatcat ccccgccagc gcaccgttgc gcgtcatgcg tgaccacatc accgagaaca
     7321 gcaccaccgg accaaacgcc gcgccaaagc ctgcccacgc gtagctcact aagcccagca
     7381 cgcggttttc cgggtttgcc gccagcgcaa tcgccaccag cgccaccacc agcaccatca
     7441 cacgtcctac ccacaccagc tctttctggc tggcctgttt acgcagaaac gctttgtata
     7501 aatcttcggt aattgcactg gaacatacca gcagctggca acttaaggtt gacattaccg
     7561 ccgccagaat cgccgacagc agaatcccgg caatccacgg gttaaacaga atttgcgcca
     7621 gttcgataaa cacgcgctcg gcgttctggt ttaccgcgcc tgctaccgcc gggtgctcat
     7681 taaagtaagc aatcccaaag aagccaacag ccaccgcccc tgccaggcag aggatcatcc
     7741 aggtcatact gatacgacgc gcatggacaa tgctgtggtg agagtctgcc gccataaaac
     7801 gcgccaggat gtgcggctgc ccgaagtaac ccagccccca acccatcagt gagataatgg
     7861 cgacaaagtt cagccctttg agcatatcaa cgttttcgat gctcttttgt ttgatcactt
     7921 ccagcgagtc accaaagcca ccgacactga taatgacgat aaccggcgtc aggatcaggg
     7981 caaaaatcat caggctggcc tgtacagtgt cagtccagct caccgcgagg aaaccgccaa
     8041 taaaggtgta aaggatcgtc gccgcagctc ccgcccacag agccgtttcg tagctcatgc
     8101 caaaggtact ttcaaacaga cgcgcgcctg ccacaatgcc cgaagcgcaa taaatggtga
     8161 agaacagcaa aatcaccagc gcggagataa tgcgcaaaat gcggctttta tcttcaaagc
     8221 gcccggtgaa ataatccggc aatgttaagg cgttattgtt gtattcggta tgcacacgca
     8281 accgccccgc caccagcttc cagttgatcc acgcgcctaa tgtcaggcca atggcgatcc
     8341 aactttcgga aatcccggaa agaaaaaccg cgcccggcaa ccccattaac agccagccgc
     8401 tcatatccga cgcacccgcc gataatgccg tcacgaatgg cccaaggcta cgaccgccca
     8461 gaatatagtc gtcaaagttt ttcgttgatc gccaggcgat aaacccaatc aatatcatgc
     8521 caaagatata gacacaaaat gtcaccaaca tcggtgtgct aatagccatc taaagtctcc
     8581 aaaaaattat tatcggcaat gtcgaaactt gccgttatat ctgccaccgg aacggggtaa
     8641 cagagtttat gttttaccag ggcgaccgta tcctgccgga agcgctggtt attcacaatc
     8701 gatttaacac accatttaca ttaaatttta gtgctcagcg acactatttt tcatcaggtt
     8761 gcactctctc acattttttg cggttgcacc tttcaaaaat gttaactgcc gcagagaaaa
     8821 agtctgagtt atttttttaa tccctgtcat atcgatttct tttattaaca tttcattcat
     8881 ttttaagctt gctacgcatg tcacatttaa catggttgca caaagttgca acatcatgga
     8941 tatttcacga taacgttaag ttgcaccttt cagaacaaca ggagtaatgg catgggaacc
     9001 accaccatgg gggttaagct ggacgacgcg acgcgtgagc gtattaagtc tgccgcgaca
     9061 cgtatcgatc gcacgccaca ctggttaatt aagcaggcga ttttttctta tctcgaacaa
     9121 ctggaaaaca gcgatactct gccggagcta cctgcgctgc tttctggcgc tgccaatgag
     9181 agcgatgaag caccgactcc ggcagaggaa ccacaccaac cattcctcga ctttgccgag
     9241 caaattttgc ccctgtcggt ttcccgcgcc gcgatcacag cggcctatcg ccgcccggaa
     9301 accgaagcgg tttctatgct gctggaacaa gcccgcctgc cgcagccagt tgctgaacag
     9361 gcgcacaaac tggcgtatca actggccgat aaactgcgta atcaaaaaaa cgccagtggt
     9421 cgcgcaggta tggttcaggg gttattgcag gagttttcgt tgtcatcgca ggaaggcgtg
     9481 gcgctgatgt gtctggcgga agcgttgttg cgtattcccg acaaagccac ccgtgatgcg
     9541 ttaattcgcg acaaaatcag caacggtaac tggcaatcac acattggtcg tagcccgtct
     9601 ttgtttgtta atgccgccac ctgggggctg ctgtttactg gcaaactggt ttccacccat
     9661 aacgaagcca gcctctcccg ctcgctgaac cgcattatcg gtaaaagcgg tgaaccgctg
     9721 atccgcaaag gtgtggatat ggcgatgcgc ctgatgggcg agcagttcgt cactggcgaa
     9781 accatcgcgg aagcgttagc caatgcccgc aagctggaag agaaaggttt ccgttactct
     9841 tacgatatgc ttggcgaagc tgcactgact gccgcagatg cacaagcgta tatggtttcc
     9901 tatcagcagg cgattcacgc cattggtaaa gcgtccaatg gtcgtggcat ctatgaaggt
     9961 ccgggcattt caatcaagct gtcggcgctg catccgcgtt acagccgcgc ccaatatgac
    10021 cgggtaatgg aagagcttta cccacgtctg aaatcactca ccctgctggc gcgtcagtac
    10081 gatattggta tcaacattga cgccgaagag gccgatcgtc tggagatctc cctcgatctg
    10141 ctggaaaaac tctgtttcga gcctgagctg gcaggctgga acggtatcgg ttttgttatt
    10201 caggcttatc aaaaacgctg cccactggtg attgattacc tgattgatct cgccacccgc
    10261 agccgtcgcc gtctgatgat tcgcctggtg aaaggcgcgt actgggatag tgaaatcaag
    10321 cgtgcgcaga tggacggcct tgaaggctat ccggtttata cccgcaaggt gtataccgac
    10381 gtttcatatc tcgcctgtgc gaaaaagctg ctggcggtgc cgaatctaat ctacccgcag
    10441 ttcgcgacgc acaacgccca tacgctggca gcgatttatc aactggcagg tcagaactac
    10501 tatccgggtc agtacgagtt ccagtgcctg catggtatgg gcgagccact gtatgagcag
    10561 gtcaccggga aagttgccga cggcaaactt aaccgtccgt gtcgtattta tgctccggtc
    10621 ggtacccatg aaacgctgtt ggcgtatctg gtgcgtcgcc tgctggaaaa cggtgctaac
    10681 acctcgtttg ttaaccgtat tgccgacacc tctttgccac tggatgaact ggtcgccgat
    10741 ccggtcactg ctgtagaaaa actggcgcaa caggaagggc aaactggatt accgcatccg
    10801 aaaattcccc tgccgcgcga tctttacggt cacgggcgcg acaactcggc agggctggat
    10861 ctcgctaacg aacaccggct ggcctcgctc tcttctgccc tgctcaatag tgcactgcaa
    10921 aaatggcagg ccttgccaat gctggaacaa ccggtagcgg caggtgagat gtcgcccgtt
    10981 attaaccctg cggaaccgaa agatattgtg ggctttgtgc gtgaagccac gccgcgtgag
    11041 gtagaacagg cgctggaaag tgcggttaat aacgcgccaa tctggtttgc cactcctccg
    11101 gttgaacgcg cggcgatttt gcatcgcgct gccgtgctaa tggaaagcca gatgcagcaa
    11161 ctgattggta ttctggtgcg tgaagccgga aaaaccttca gtaacgccat tgccgaagtg
    11221 cgcgaagcgg tcgattttct ccactactat gccggacagg tgcgggatga tttcgctaac
    11281 gaaacccacc gtccattagg gcctgtggtg tgtatcagtc cgtggaactt cccgctggct
    11341 attttcaccg ggcagattgc tgccgcactg gcggcaggta acagcgtgct ggcaaaaccg
    11401 gcagaacaaa cgccgctgat tgccgcgcaa gggatcgcca ttttgctgga agcgggtgta
    11461 ccgccaggcg tggtgcagtt gctgccaggt cagggggaaa ccgtgggcgc gcaactgacg
    11521 ggtgatgatc gcgtacgcgg ggtgatgttt accggttcaa ccgaagtcgc tacgttactg
    11581 cagcgcaata tcgccagccg cctggacgct cagggtcgcc ctattccgct catcgctgaa
    11641 accggcggca tgaacgcgat gattgtcgat tcttcagcac tgaccgaaca ggtcgtcgtg
    11701 gatgtactgg cctcggcgtt cgacagtgcg ggtcagcgtt gttcggcgct gcgcgtgctg
    11761 tgcctgcaag atgagattgc cgaccacacg ttgaaaatgc tgcgcggcgc aatggccgaa
    11821 tgccggatgg gtaatccggg tcgcctgacc accgatatcg gtccggtgat tgatagcgaa
    11881 gcgaaagcca atattgagcg ccatattcag accatgcgta gcaaaggccg tccggtgttc
    11941 caggcggtgc gggaaaacag cgaagatgcc cgtgaatggc aaagcggcac ctttgtcgcc
    12001 ccgacgctga tcgaactgga tgactttgcc gaattgcaaa aagaggtctt tggtccggtg
    12061 ctgcatgtgg tgcgttacaa ccgtaaccag ctaccagagc tgatcgagca gattaacgct
    12121 tccggctatg gtctgacgct tggtgtccat acgcgtattg atgaaaccat cgcccaggtc
    12181 actggctcgg cccatgttgg taacctgtat gttaaccgta atatggtggg cgcagtggtg
    12241 ggtgtgcagc cattcggcgg cgaagggttg tccggtaccg ggccgaaagc aggcggtccg
    12301 ctctatctct accgtctgct ggcgaatcgc ccggaaagtg cgctggcagt gacgctcgcg
    12361 cgtcaggatg cagagtatcc ggtcgatgca cagttgaaag ccgcattgac tcagccgcta
    12421 aatgcactgc gggaatgggc agcaaatcgt ccagaattgc aggcgttatg tacgcaatat
    12481 ggcgagctgg cgcaggcagg aacacaacga ttgctgccgg ggccgacggg tgaacgcaac
    12541 acctggacgc tgctgccgcg tgagcgcgtg ttgtgtattg ccgatgatga gcaggatgcg
    12601 ctgactcagc tcgccgccgt gctggcggtg ggcagccagg tactgtggcc ggatgacgcg
    12661 ctgcatcgtc agctagtaaa ggcattgcca tcggcagtca gcgaacgtat tcaactggcg
    12721 aaagcggaaa atataaccgc tcaacccttt gatgcggtga tcttccacgg tgattcggat
    12781 cagcttcgcg cattgtgtga agccgttgct gcgcgggatg gcgcaattgt ttcggtgcag
    12841 ggttttgccc gtggcgaaag caatatcctt ctggaacggc tgtatatcga acgttcgctg
    12901 agtgtgaata ccgctgccgc tggcggtaac gccagtttaa tgactatagg ttaaacaggt
    12961 agccggagga tgttacaacc tcctccggca tctttaacgt ggtcgaatcc cctcaataat
    13021 aatccgctgc acgttttcaa ccgtttgatt gaaaaatatc tcatcgcgca acgtcgcgcc
    13081 tgtcaccgcc tccacctgag gggcgaaatc ggcgtaatgt tgagtggaag cccaaatcat
    13141 gaaaatcaaa tgctgcggat caatcggcgc gagtttgccg cttttgaccc aaccggcaat
    13201 cagcgccgat ttctcatcaa ttaatgcctt caaatcgccc gtcagttcat ccattaacag
    13261 cggcgcgcct gccagcatct ccatacagaa caggcgcgaa gcctgcggat aatcgcggga
    13321 gacttccagc ttcagacgga tgtactcttt tatcgccgcc agcggagcga aatcttcacg
    13381 aaacgctttt aacggtgcca gccagatatc gagaatctgc cgcagcacgg caatatacag
    13441 cgcctctttt gacggaaagt aatacagcag attggttttt gaaacacccg ccaactctgc
    13501 gatctgctcc agccttgtgc cgtgaaaacc gaattgtgaa aaagtgtcca gtgctgcgct
    13561 aagaatcgct tttttcttcg cgcttactgc gcgcgaacgt ttacccgttg ttttcactgc
    13621 gccttgcgtc atgcgctctc ccctcttgta gattggcaac aggatagcaa aacccccgcc
    13681 cggcctcgac cttctgcact ctcatcgcgc acagatgcat gttttatgtg caactgtttt
    13741 gaccgtttag tccacttttt accagatatt ttagacaaca caatgttaac acattaataa
    13801 cattcacttt ttaaaaactg gcatccgctt tgcaaacaag ccattacgcg gctgaaaagg
    13861 atatgcagga tgcagcgccc cgtctgactt tcactttacg agacgaagag aggttgatga
    13921 tgaaaattgg cgtattcgta cctattggca acaacggctg gctcatttcg acccacgcgc
    13981 cgcagtacat gccgaccttt gaactgaata aagccattgt gcaaaaagcg gagcactacc
    14041 atttcgattt cgccctgtcg atgatcaaac tgcgtggctt tggcggcaaa actgagttct
    14101 gggatcacaa ccttgagtcg ttcaccttga tggcggggct ggcggccgtc acctcgcgca
    14161 ttcagattta cgccactgct gccaccttaa cgttacctcc ggcaatcgtc gcccgtatgg
    14221 ccgcaaccat cgactccatc tctggcgggc gttttggcgt caacctcgtg actggctggc
    14281 aaaagcccga gtatgagcag atgggtatct ggcctggcga tgactatttc tcccgtcgtt
    14341 acgactatct caccgaatat gttcaggtgc tgcgcgacct gtggggcacg gggaaaagcg
    14401 attttaaagg cgattttttc accatgaatg attgtcgcgt cagtccgcaa ccgagtgtcc
    14461 ccatgaaagt gatctgcgcc gggcaaagcg acgctggcat ggcgttctcc gcccagtatg
    14521 ccgatttcaa cttctgtttc ggcaaaggcg taaatacgcc cacggctttc gccccgaccg
    14581 ctgcgcggat gaaacaggcc gcagagcaaa ccgggcgcga cgttggttct tatgtgttgt
    14641 ttatggtgat tgccgatgaa accgacgatg ccgctcgcgc caaatgggaa cactacaaag
    14701 cgggcgcgga tgaagaggcg ttaagctggc taaccgaaca aagtcagaaa gatacccgct
    14761 ccggtactga taccaacgtc cgtcagatgg ccgatcccac ttcggcggta aacatcaata
    14821 tggggacgtt agtcggttct tacgccagtg tcgcgcgcat gttagatgaa gtcgcaagcg
    14881 tgcctggtgc cgaaggcgtg ctgttgacct tcgatgattt tctgtcggga atcgaaacct
    14941 tcggcgagcg cattcaacca ctgatgcagt gccgcgccca tctacctgcg ctgactcagg
    15001 aggtggcatg atgacaacct taaccgctcg accggaagcc attaccttcg atccgcagca
    15061 aagtgcgctg atcgtggtgg atatgcaaaa cgcttatgcc acgccaggcg gctacttaga
    15121 tctcgccggg tttgatgtct caaccactcg cccggtcatt gccaacattc aaaccgccgt
    15181 gaccgcagcg cgagcggcag ggatgctgat catctggttt caaaatggct gggatgaaca
    15241 gtatgtcgaa gctggcggcc ccggctcacc gaattttcat aaatcgaacg ccctgaaaac
    15301 catgcgtaag cagccgcagc tgcaggggaa attgctggcg aaaggctcct gggattatca
    15361 actggtggat gaactggtgc cgcagcctgg cgatattgtg ctgccgaagc cgcgctacag
    15421 cggtttcttc aatacaccgc tggacagcat tttgcgcagc cgtggaatac gccatctggt
    15481 tttcaccggt atcgctacca acgtctgcgt cgaatcgacg ctacgcgatg gcttttttct
    15541 ggagtatttc ggcgtggtgc tggaagacgc aacacaccag gcggggccgg aatttgcaca
    15601 gaaagccgcg ttgttcaata tcgaaacctt ttttggctgg gtcagcgacg tcgaaacgtt
    15661 ctgcgacgcg ctttctccca catcctttgc tcgtatcgct taaggagttt aacgatgcca
    15721 aaatccgtaa ttattcccgc tggcagcagc gcaccgctgg cccccttcgt tcccggcacg
    15781 ctggctgatg gcgtggtgta tgtctccggt acgctggctt ttgatcaaca taataacgtg
    15841 ctgtttgccg atgacccaaa ggcgcaaacc cgccacgttc tggaaattat ccgcaaggtg
    15901 atcgagacgg cgggtggcac gatggcggat gtgaccttca acagcatctt tattaccgac
    15961 tggaaaaatt acgccgcgat taacgaaatc tacgccgagt tttttccggg tgataaaccg
    16021 gcgcgattct gcattcagtg cggactggta aaacctgacg cgctggtgga aatcgccaca
    16081 attgcgcata tcgccaagtg aggccgcgat gaaactttca ctctcacctc ccccttatgc
    16141 tgatacgccc gtagtggtgt tgatttcggg tcttggcggt agcggcagtt actggttacc
    16201 gcaactggcg gtgctggagc aggagtatca ggtagtctgt tacgaccagc gcggcaccgg
    16261 caataatccc gacacgctgg cagaagatta cagtatcgcc cagatggcag cggaactgca
    16321 tcaggcgctg gtagccgcag ggattgagca ttacgcggtg gtcggccatg cgctcggtgc
    16381 gctggtggga atgcagctgg cgctggatta tcccgcgtcg gtaactgtgc tgatcagcgt
    16441 taacggctgg ctacgaataa acgcccatac gcgccgctgt tttcaggttc gcgaacgatt
    16501 actgtatagc ggcggcgcgc aggcatgggt ggaagcgcag ccgttgttcc tctatcccgc
    16561 cgactggatg gcggcccgcg cacctcgcct ggaggcagaa gacgcgctgg cactggcgca
    16621 ttttcagggc aaaaataatt tactgcgtcg acttaacgcc ctcaaacgcg ctgactttag
    16681 tcaccatgcg gatcgcatcc gctgcccggt gcaaatcatc tgcgccagtg atgatctgct
    16741 ggtgccatca gcatgttcca gtgagcttca tgccgccctg cccgatagcc agaaaatggt
    16801 gatgccctat ggcggacacg cctgcaacgt gaccgatccc gaaacgttta atgctctgtt
    16861 actcaacggg cttgccagcc tgttacatca ccgtgaagcc gccctgtaag gaattgctat
    16921 gaacgaagcc gttagcccag gtgcgcttag caccttgttc accgatgccc gcactcacaa
    16981 cggctggcgg gagacacccg tcagcgatga gacgttacgg gagatttatg ccctgatgaa
    17041 atgggggccg acatcagcta actgttctcc ggcacggatc gtgtttatcc gcacggcaga
    17101 aggaaaagaa cgtctgcgcc cggcactttc cagcggcaat ctgcaaaaaa ccctgaccgc
    17161 gcccgtcacc gctatcgtcg cctgggacag tgaattttat gaacggttac cgcaactgtt
    17221 tcctcacggc gatgcccgca gttggtttac ctccagccca caacttgccg aagaaacagc
    17281 gtttcgcaac agttccatgc aggcggccta tctgatcgtc gcctgccgtg cgctgggact
    17341 ggataccggc ccgatgtcgg gttttgaccg tcaacacgtt gacgacgcct tttttgcggg
    17401 cagcacgctg aagagcaatc tgctgattaa tatcggctat ggcgatagca gcaaactttt
    17461 tgcgcgcctg ccacgtctgt cctttgaaga agcctgcggg ctgttgtaag gagcaaacca
    17521 tgaacattgt cgatcaacaa acctttcgcg atgcgatgtc ctgtatgggc gcggcagtca
    17581 atatcatcac cacggatggt ccagccgggc gcgccgggtt caccgccagc gcggtctgca
    17641 gcgtgaccga cacgccgcct acattactgg tgtgcctgaa tcgtggggcg tccgtctggc
    17701 cggtattcaa tgaaaatcga acgctgtgtg taaatacact gagcgccggg caggagccgc
    17761 tttcaaatct ttttggcggc aaaacgccca tggaacaccg ctttgccgcc gcccgctggc
    17821 agacgggtgt gaccggatgc ccgcaactgg aagaggctct ggtttcgttt gactgccgta
    17881 tcagccaggt ggtgagcgtc ggcacccacg acattctgtt ttgcgccatc gaagcgattc
    17941 atcgtcacgc cacaccctac gggctggtgt ggttcgatcg cagttatcac gcgctgatgc
    18001 gccctgcttg ttaacctcct aaaggagaca gctgatggca atgttcggtt ttcctcactg
    18061 gcagttaaaa tcgacctcta cagagagcgg cgtggtcgcg ccgtatgaac gattgccgtt
    18121 tgctcagacg gcagtaatgg gcgttcaaca cgcggtggcg atgtttggcg cgacggtgtt
    18181 aatgccgatt ctgatggggc tggatcccaa tctttccatt ttaatgtcgg ggatcggcac
    18241 gttactgttt ttctttatta ccggcgggcg cgtccccagt tatctcggtt ccagcgcggc
    18301 ttttgtcggc gtggtgattg ccgcgacggg ctttaacggt cagggcatta acccgaatat
    18361 cagcattgcg ctgggcggga ttatcgcctg tgggctggtt tataccgtca ttggcctggt
    18421 ggtgatgaaa attggtacac gctggattga acgactaatg ccgccggtgg tgacgggtgc
    18481 ggtggtgatg gcgattggcc tgaacttagc gcctatcgca gtgaaaagtg tatcggcttc
    18541 ggcctttgat agctggatgg cggtaatgac ggtgctgtgt attgggctgg tggcggtgtt
    18601 tacccgtggc atgatccagc ggttattgat tctcgtaggt ttaattgtcg cctgtctgct
    18661 gtatggcgtg atgaccaacc ttttagggct gggaaaagcc gtcgatttta ctctcgtcag
    18721 ccatgctgcc tggttcggtt tgccacattt ttccactcct gcttttaata gtcaggcaat
    18781 gatgctgatt gcgcccgtgg cggtgattct ggtggcggaa aacttaggtc atctcaaggc
    18841 ggtcgccggg atgaccggac gcaatatgga cccgtatatg gggcgggcat tcgtcggtga
    18901 tgggctggcg acgatgcttt ctggttctgt cggtggcagc ggcgtaacca cctacgccga
    18961 gaatatcggt gtaatggccg tgacaaaagt ctactcgacg ctggtgtttg tcgctgctgc
    19021 cgtcatcgcc atgctgttgg gcttttcacc gaaatttggc gcgctgattc ataccattcc
    19081 ggccgcggtg attggtggtg catcaattgt tgtgttcggg ctgattgccg tcgcaggcgc
    19141 aaggatatgg gtacaaaacc gtgtcgattt aagccagaac ggtaatttga ttatggtcgc
    19201 agtaacctta gtgctgggcg cgggtgattt tgctctcacg ctgggcggtt ttacgttggg
    19261 agggattggt acagcaacct ttggcgcgat tttactcaat gcgttgttaa gcagaaagtt
    19321 ggttgacgtt ccaccgccag aagttgttca tcaggagcca taaacagaaa accgccggga
    19381 ttaccggcgg tgatgacttt ttgacgagcc taagaaaaaa atgcattcgt cattgattag
    19441 cccggctagt tgtcgctttt gccgtgactg ctcttgccac cttttttacc tgcttcagat
    19501 gcgcgctgcg ggtcattctt gaaattaccc ccgctgtgct gtccaccttt tttacctgct
    19561 tctgatgctc tttcgcggtc ttctgcaaaa ttgccggaac cgcctcgatg gtttgccatt
    19621 actgacctcc gtctgggtat agacatcata ttgcatagaa ttaacgcggc atatggcggc
    19681 tgccatattt tcgctacgtg ggactaagct tagtgaactg acgcgggcag acccgcgttt
    19741 ggtaatatcc tgcaacagct tcttagcgag aatagatatc atctcaaata attatccata
    19801 agccgctctt atgattaaaa aaacgcgatg agataaaaat gtgtcatttt gcgacaaaat
    19861 tacgtgcttg tgaaagttgt tataaatcaa ataagtggtt gtgaaatttg cactctgaaa
    19921 aggacgtctt atctttaaat aagtggtagc gaatcgctac ggaatagaga taacacgagg
    19981 agtggttaga aatggctaaa gttctggtgc tttattattc catgtacgga catattgaaa
    20041 cgatggcacg cgcagtcgct gagggtgcaa gcaaagtcga tggcgcagaa gttgtcgtta
    20101 agcgtgtacc ggaaaccatg ccgccgcaat tatttgaaaa agcaggcggt aaaacgcaaa
    20161 ctgcaccggt tgcaaccccg caagaactgg ccgattacga cgccattatt tttggtacac
    20221 ctacccgctt tggcaacatg tccggtcaaa tgcgtacctt cctcgaccag acgggcggcc
    20281 tgtgggcttc cggcgcacta tacggaaaac tggcgagcgt ctttagttcc accggtactg
    20341 gcggcggtca ggaacaaact attacttcaa cctggacgac ccttgcgcat cacggcatgg
    20401 taattgtccc cattggctac gcagcgcagg aattatttga cgtttcacag gttcgcggcg
    20461 gtacgccgta cggcgcaacc accatcgcag gcggtgacgg ctcacgccag ccaagccagg
    20521 aagaactgtc tattgctcgt tatcaagggg aatatgtcgc aggtctggca gttaaactta
    20581 acggctaatc ttcaacagga ggatacgcat gccaactcaa gaagcgaaag ctcatcacgt
    20641 cggtgaatgg gcatctctgc gcaatacgtc gccggaaata gccgaggcca ttttcgaagt
    20701 cgccgggtac gacgaaaaaa tggcggaaaa gatttgggaa gaaggtagcg atgaagtgtt
    20761 agtcaaagcc tttgctaaga ccgataaaga ttcgcttttt tggggcgaac agaccatcga
    20821 acgtaaaaac gtttaaccag cgactccccc acttctcgcg ggggagtttt ctgttatttc
    20881 accgcctcat tcaacacgct atcaaactta tccattgggc agaaaccgtt ggcatctatc
    20941 gggcaaccgc ttaattccag cgtcacacgc tgagcaggtg cctgcagggt taacgcatcg
    21001 gcattacgta actgttccgc actctgatac acatattcaa ttttcatcaa atcgcgattg
    21061 gctttgctgt catgccaacg ctggaaaacg attttgccgc caatcggcgt gcgttcgttc
    21121 tggtcatgca actgatacgg tttgaaatcc agcgccgtta acagagaggc aatgttggag
    21181 tcgtgcccaa ccaacactgt aattttcggt gcgctggtgc gatcggtgac cagagctttg
    21241 tcgatataac tgaccagcgg tttcgcaaca ttgcgcgcca cttccggtga ggtaaacagg
    21301 ctgtcctggt agccgttttt aagcttcgac aacaccttcc actgctggtc agatttgatt
    21361 tctccccagg ccacctgatc catcggaaaa ccttcgtaat attgcaaagt aaacgcatct
    21421 accagcgagt tgccgacttt cagcggcccg gaaacacctg gttcttgttg atacttggcg
    21481 ctaaaggtat ttttgccatc caccagcgaa cactgttgtt tctctttaca ggcaggggaa
    21541 tctttatagt taacgatttt ttccagtagt tggtagctgt cggtaagctg gagtttgctg
    21601 agctctttct ccattgccgc caccgcctgt tcactgaatg cggcggaatc atcggtgatc
    21661 accgggttaa aggttgggtc catagtgccc attttttcct ggtgatgcac aggaatatca
    21721 caccccggga atgcgccggt aataaagaac tgtgcggtcg caacggtacg ttgcagacta
    21781 ttggcatagg cataaacggt gtccggcggc gggcattccc ccgatttcac catcccctgc
    21841 tgtgccagcc attcacgcat gtaatggccc atatacactt cgagcacgcc acctttggtg
    21901 gtgagttgcc caccggggac gtcccattcg ggccatttat tcggcgtaga ctgctccagc
    21961 acactgccat tgttcgccag cggcgcacgt aagttatggc ggctcatcat gagcacttgc
    22021 tgtagctgat agccttccgg tacggtttgt gcctgagcat ttgaagcgag taaaactatc
    22081 cctgccacag ttgcggcgat tagcgttttg ttcattcctg gcacctcttt tgttatcaat
    22141 cactaaagag tgtgacagaa atatgacatt tttccgggaa caggttcgca aaatggcttg
    22201 ttgaatgcct gtttttctat atacatcttt aaaaacaggc aaggtttgac gcaaactatg
    22261 cctgattagc tccctgcctc gcgtacgccg ctgatgatta cacctgtggc taaacagaat
    22321 atcagcggcg ttactaatac agaggcaata gtaccaataa taagagaagt gaggctaata
    22381 acaatggttt ttagcacatg cctttgtcag taaattgcat aacgagttaa aaaatcaatt
    22441 tgcgcattcc tttccttatt gtttaactcg taagcctgag caaaaagttc aggagggagg
    22501 taaacgtcat aatgattgcc agtgtttatt aaatacccat actcaaggtc gtcatctgtt
    22561 gaaaaggatt cattccctaa ggagattgat gccacattac cattaacaat aaataatgag
    22621 caaatgctat ctgaaagagc agtgaggaaa tttttcacct cattaacctt ctgctctggg
    22681 tcggtgtct
//
LOCUS       DAZFWL020000071        22355 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFWL020000071 DAZFWL020000000
VERSION     DAZFWL020000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.071"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(38..199)
                     /gene="rsmS"
                     /locus_tag="ACHKNP_RS14480"
                     /old_locus_tag="pgaptmp_002896"
     CDS             complement(38..199)
                     /gene="rsmS"
                     /locus_tag="ACHKNP_RS14480"
                     /old_locus_tag="pgaptmp_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HGU4705025.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(213..740)
                     /gene="priC"
                     /locus_tag="ACHKNP_RS14485"
                     /old_locus_tag="pgaptmp_002897"
     CDS             complement(213..740)
                     /gene="priC"
                     /locus_tag="ACHKNP_RS14485"
                     /old_locus_tag="pgaptmp_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HGU4705026.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            810..1187
                     /gene="ybaN"
                     /locus_tag="ACHKNP_RS14490"
                     /old_locus_tag="pgaptmp_002898"
     CDS             810..1187
                     /gene="ybaN"
                     /locus_tag="ACHKNP_RS14490"
                     /old_locus_tag="pgaptmp_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HGU4705027.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            1340..1891
                     /gene="apt"
                     /locus_tag="ACHKNP_RS14495"
                     /old_locus_tag="pgaptmp_002899"
     CDS             1340..1891
                     /gene="apt"
                     /locus_tag="ACHKNP_RS14495"
                     /old_locus_tag="pgaptmp_002899"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGU4705028.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            2020..3951
                     /gene="dnaX"
                     /locus_tag="ACHKNP_RS14500"
                     /old_locus_tag="pgaptmp_002900"
     CDS             2020..3951
                     /gene="dnaX"
                     /locus_tag="ACHKNP_RS14500"
                     /old_locus_tag="pgaptmp_002900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGU4705029.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    3284..3348
                     /gene="dnaX"
                     /locus_tag="ACHKNP_RS14500"
                     /old_locus_tag="pgaptmp_002900"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            4004..4333
                     /gene="ybaB"
                     /locus_tag="ACHKNP_RS14505"
                     /old_locus_tag="pgaptmp_002901"
     CDS             4004..4333
                     /gene="ybaB"
                     /locus_tag="ACHKNP_RS14505"
                     /old_locus_tag="pgaptmp_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGU4705030.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            4333..4938
                     /gene="recR"
                     /locus_tag="ACHKNP_RS14510"
                     /old_locus_tag="pgaptmp_002902"
     CDS             4333..4938
                     /gene="recR"
                     /locus_tag="ACHKNP_RS14510"
                     /old_locus_tag="pgaptmp_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGU4705031.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            5048..6922
                     /gene="htpG"
                     /locus_tag="ACHKNP_RS14515"
                     /old_locus_tag="pgaptmp_002903"
     CDS             5048..6922
                     /gene="htpG"
                     /locus_tag="ACHKNP_RS14515"
                     /old_locus_tag="pgaptmp_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HGU4705032.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVELEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            7103..7747
                     /gene="adk"
                     /locus_tag="ACHKNP_RS14520"
                     /old_locus_tag="pgaptmp_002904"
     CDS             7103..7747
                     /gene="adk"
                     /locus_tag="ACHKNP_RS14520"
                     /old_locus_tag="pgaptmp_002904"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGU4705033.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            7983..8945
                     /gene="hemH"
                     /locus_tag="ACHKNP_RS14525"
                     /old_locus_tag="pgaptmp_002905"
     CDS             7983..8945
                     /gene="hemH"
                     /locus_tag="ACHKNP_RS14525"
                     /old_locus_tag="pgaptmp_002905"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGU4705034.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKMYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(8942..9901)
                     /gene="aes"
                     /locus_tag="ACHKNP_RS14530"
                     /old_locus_tag="pgaptmp_002906"
     CDS             complement(8942..9901)
                     /gene="aes"
                     /locus_tag="ACHKNP_RS14530"
                     /old_locus_tag="pgaptmp_002906"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HGU4705035.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            10053..11357
                     /gene="gsk"
                     /locus_tag="ACHKNP_RS14535"
                     /old_locus_tag="pgaptmp_002907"
     CDS             10053..11357
                     /gene="gsk"
                     /locus_tag="ACHKNP_RS14535"
                     /old_locus_tag="pgaptmp_002907"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HGU4705036.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(11490..13166)
                     /gene="ybaL"
                     /locus_tag="ACHKNP_RS14540"
                     /old_locus_tag="pgaptmp_002908"
     CDS             complement(11490..13166)
                     /gene="ybaL"
                     /locus_tag="ACHKNP_RS14540"
                     /old_locus_tag="pgaptmp_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HGU4705037.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(13404..14624)
                     /gene="fsr"
                     /locus_tag="ACHKNP_RS14545"
                     /old_locus_tag="pgaptmp_002909"
     CDS             complement(13404..14624)
                     /gene="fsr"
                     /locus_tag="ACHKNP_RS14545"
                     /old_locus_tag="pgaptmp_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HGU4705038.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            14842..16494
                     /gene="ushA"
                     /locus_tag="ACHKNP_RS14550"
                     /old_locus_tag="pgaptmp_002910"
     CDS             14842..16494
                     /gene="ushA"
                     /locus_tag="ACHKNP_RS14550"
                     /old_locus_tag="pgaptmp_002910"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HGU4705039.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGKMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGRLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(16531..17010)
                     /gene="ybaK"
                     /locus_tag="ACHKNP_RS14555"
                     /old_locus_tag="pgaptmp_002911"
     CDS             complement(16531..17010)
                     /gene="ybaK"
                     /locus_tag="ACHKNP_RS14555"
                     /old_locus_tag="pgaptmp_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HGU4705040.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(17214..18008)
                     /gene="ybaP"
                     /locus_tag="ACHKNP_RS14560"
                     /old_locus_tag="pgaptmp_002912"
     CDS             complement(17214..18008)
                     /gene="ybaP"
                     /locus_tag="ACHKNP_RS14560"
                     /old_locus_tag="pgaptmp_002912"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HGU4705041.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            18146..18487
                     /gene="ybaQ"
                     /locus_tag="ACHKNP_RS14565"
                     /old_locus_tag="pgaptmp_002913"
     CDS             18146..18487
                     /gene="ybaQ"
                     /locus_tag="ACHKNP_RS14565"
                     /old_locus_tag="pgaptmp_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4705042.1"
                     /translation="MKQATRKPTTPGDILLYEYLVPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(18601..21105)
                     /gene="copA"
                     /locus_tag="ACHKNP_RS14570"
                     /old_locus_tag="pgaptmp_002914"
     CDS             complement(18601..21105)
                     /gene="copA"
                     /locus_tag="ACHKNP_RS14570"
                     /old_locus_tag="pgaptmp_002914"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HGU4705043.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            21367..22299
                     /gene="glsA"
                     /locus_tag="ACHKNP_RS14575"
                     /old_locus_tag="pgaptmp_002915"
     CDS             21367..22299
                     /gene="glsA"
                     /locus_tag="ACHKNP_RS14575"
                     /old_locus_tag="pgaptmp_002915"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HGU4705044.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT         5496 a         5973 c         5906 g         4980 t
ORIGIN      
        1 acaatcttag gggacattta gcggccccat ttatttctca cttttccgcc tcatcatcgc
       61 gcgttaattt cttttcataa tcgcgtttta caatatccag cgcgcgcagc acggtgcggg
      121 ctgggatctg attttcttcc agcagcacaa tcaaatcgac agccagtttg acatcgtcag
      181 gggcattttc cagtgacata ctctctccat tgctagcggg ttaagcgcgc tagcctgttt
      241 tcgatttttt ccagcgcatg gcggcagcgt gccaggcgcg cttcataggc ttccacttca
      301 cgatgcagcg tttgctgttc cacgagatcg gtcacccgcg ccaggcgggc tctgcgttcg
      361 gcaaccatct cacgtagccg ccgctcaaaa tcctgatgct gaatacgttt acgctgccag
      421 cgggcaattt tcggtggcgc actgtcccac tcgcgcaatg accaggcgga ggcttcacgc
      481 gcgatggctt ccagttgtgc cgccagatgt tccgccagcc aggccacttg cggcagctgt
      541 tgctgctcaa ctgcatgacg aagcgcagcc agattatcgc ccgcctcatc gagacaagcc
      601 tgcagtgttg tcgcacgggt ttgaaaaagg tgtctgtcaa aacgggcact tagcgtggca
      661 aattgcgcta ccggggcaca acgctgacgc agcgtagcga gctgtccttc cagtttttcc
      721 agcagcaggg cggttttcac gataagacct caatgaaaat gataattgtt atgctaaagt
      781 agccactctg taagctgaca acactcaata tgcaacgaat cattttaatc atcattggct
      841 ggctggcggt agtgctgggt acgctgggcg tggtattacc ggtattaccg acgacgccgt
      901 ttatcctgct ggcggcctgg tgctttgccc gttcttcccc gcgctttcac gcctggttgc
      961 tgtaccgctc atggtttggc agctatctac gtttctggca gaaacatcat gcgatgccgc
     1021 gcggcgtcaa accgcgggcg attttgctta ttttgctcac gtttgccatt tctctgtggt
     1081 tcgtccagat gccatgggtg cgcatcatgt tgctggtaat tctcgcctgt ttgcttttct
     1141 atatgtggcg aattccggtg attgatgaaa agcaagaaaa gcactgaagc acaacaatcg
     1201 cagttgcaat tattgcgtac agccagtaca ttctggcgtt ttcgagcaca ggcgcaggcg
     1261 gtcaaaggtt aaacaactgt tacttttgat acgtttaaaa cgcgccgtga gtaccaccgt
     1321 aacaagcagg catacactta tgaccgcgac tgcacagcag cttgagtatc tcaaaaatag
     1381 catcaaaagc attcaggact acccaaaacc cggcattctt ttccgcgatg tcaccagctt
     1441 actggaagac ccgaaagctt acgctctcag catcgacttg ctggttgagc gttacaaaaa
     1501 tgcgggcatt accaaagttg tcggcaccga agcgcgtggc ttcttgtttg gcgctccggt
     1561 agctctgggt ctgggcgttg gctttgtacc ggtccgtaaa ccgggcaaac tgccgcgtga
     1621 aaccatcagt gaaacttacg acctggaata cggcaccgat cagctggaga tccacgttga
     1681 tgccatcaaa ccgggcgaca aagttctggt ggtggacgat ctgctggcaa ccggcggcac
     1741 tatcgaagcg accgttaaac tgatccgtcg tctgggtggt gaagtggctg acgctgcgtt
     1801 cattatcaac ctgttcgatc tcggcggcga acagcgtctc gaaaaacagg gcattaccag
     1861 ctacagcctt gtcccgttcc cgggccatta attatcgcca gtcttgtgct gcccacgcta
     1921 cggacagcac aagatgtgca ttcagcctcg ccgttctgac ggggctgtgt tagcattacc
     1981 ccttcgtgaa tccaccttcc agcgtttcag agcctgccaa tgagttatca ggtcttagcc
     2041 cgaaaatggc gcccacaaac ctttgctgac gtcgtcggcc aggaacatgt gctgaccgca
     2101 ctggcgaacg gcttgtcgtt agggcgtatt catcatgctt atcttttttc cggtacccgt
     2161 ggcgtcggaa aaacctctat cgcccgactg ctggcgaagg ggctaaactg cgaaaccggc
     2221 attaccgcga cgccgtgcgg cgtgtgcgat aactgtcgtg aaatcgagca ggggcgcttt
     2281 gtcgatctga ttgaaatcga cgccgcctcg cgcaccaaag ttgaagatac ccgcgacctg
     2341 ctggataacg tccagtacgc tccggcgcgt ggtcgtttca aagtttatct gatcgacgaa
     2401 gtgcatatgc tgtcgcgcca cagctttaac gcactgttaa aaacccttga agagccgccg
     2461 gagcacgtta agtttctgct ggcgacgacc gatccacaga aattgccggt gacgattttg
     2521 tcgcgctgtt tgcaatttca tctcaaggcg ctggatgtcg agcaaattcg ccatcaactt
     2581 gagcacatcc tcaacgaaga acatatcgct cacgagccgc gggcgttgca attgctggcg
     2641 cgcgccgctg aaggcagcct gcgagatgcc ttaagtctga ccgaccaggc gattgccagc
     2701 ggtgacggcc aggtttcaac ccaggcagtc agtgcgatgc tgggtacgct tgacgacgat
     2761 caggcgctgt cgctggttga agcgatggtc gaggccaacg gcgagcgcgt aatggcgctg
     2821 attaatgaag ccgctgcccg tggtatcgag tgggaagcgt tgctggtgga aatgctcggc
     2881 ctgttgcatc gtattgcgat ggtacaactt tcgcctgctg cacttggcaa cgacatggcc
     2941 gccattgagc tgcggatgcg tgaactggcg cgcaccatac cgccgacgga tattcagctt
     3001 tactatcaga cgctgttgat tggtcgcaaa gaattaccgt atgcgccgga ccgccgcatg
     3061 ggcgttgaga tgacgctgct gcgcgcgctg gcgttccatc cacgtatgcc tctgcctgag
     3121 ccagaagtgc cacgccagtc ctttgcgccc gtcgcgccaa cggcagtaat gacgccaacc
     3181 caggtgccgc cgcaaccgca atcagcgccg cagcaggcac cgactgtacc gctcccggaa
     3241 accaccagcc aggtgctggc ggcgcgccag cagttgcagc gcgtgcaggg agcaaccaaa
     3301 gcaaaaaaga gtgaaccggc agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg
     3361 gaaagactgg cttcggtcac cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa
     3421 aaagcgccag ccaaaaaaga agcgtatcgc tggaaggcta ccactccagt gatgcagcaa
     3481 aaagaagtgg tcgccacgcc gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg
     3541 gaactggcgg cgaagctggc ggcagaagcc attgagcgcg acgcgtgggc ggcacaggtg
     3601 agtcaacttt cgctaccaaa actggtcgaa caggtggcct taaatgcctg gaaagaggag
     3661 agcgacaacg cagtatgtct gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt
     3721 gcacagcaaa aactggctga agcgttgagc acgttaaaag gttcaacggt tgaactgact
     3781 atcgttgaag atgataatcc cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac
     3841 gaagaaaaac ttgcgcaggc gcgcgagtcc attattgcgg ataataatat tcagaccctg
     3901 cgtcggttct tcgatgcgga gctggatgaa gaaagtatcc gccccatttg atcgtaagca
     3961 cagcttacgt tcgtcatcct taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg
     4021 tctgggtaac ctgatgaagc aagcccagca gatgcaagaa aaaatgcaga aaatgcagga
     4081 agagatcgcg cagctggaag tcaccggcga atctggcgca ggtctggtaa aagtgaccat
     4141 caacggtgca cacaactgcc gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa
     4201 agagatgctg gaagacctgg tggctgcagc attcaacgac gcagcacgtc gtattgaaga
     4261 aacgcagaaa gaaaaaatgg cctctgtatc ctccggaatg cagctgccgc ctggctttaa
     4321 gatgccgttc tgatgcaaac cagcccgctg ttaacacagc ttatggaagc actgcgctgt
     4381 ctgccgggcg ttggcccgaa gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat
     4441 cgtagcggcg ggatgcgtct ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac
     4501 tgcgccgatt gccgcacttt caccgaacag gaagtctgta acatctgttc gaatccgcgt
     4561 cgtcaggaaa acggtcaaat ctgtgtggtg gagagtccgg cggacatcta cgccattgag
     4621 cagacggggc agttttcagg ccgttatttt gtgttgatgg ggcacctgtc accgctggac
     4681 ggcatcggtc cggatgatat cggccttgat cgtctggaac agcgtctggc agaggaaaaa
     4741 atcactgaag tgatcctcgc caccaatccc acggttgaag gtgaagctac cgctaactac
     4801 attgccgagc tttgcgcgca atatgacgtg gaagccagcc gaatcgctca tggcgtaccg
     4861 gttggcggcg aactggaaat ggtcgatggc accacgctgt cacactccct tgccgggcgt
     4921 cataagattc gtttttaagc aaacgagagc aggatcacct gctctcgctt gaaattattc
     4981 tcccttgtcc ccatctctcc ctcatcctgt ttttaacctt aaaatggcat tattgaggta
     5041 gacctacatg aaaggacaag aaactcgtgg ttttcagtca gaagtgaaac agcttctgca
     5101 cctgatgatc cattctctct attccaataa agaaatcttc ctgcgtgagc ttatctctaa
     5161 cgcctccgat gcggcggaca agctgcgttt ccgtgcgcta tctaacccgg acctgtacga
     5221 aggtgatggc gaactgcgcg ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat
     5281 ctccgataac ggcgtgggga tgacccgcga cgaagtgatt gaccatctgg ggactatcgc
     5341 taaatccggt accaaatcat tcctcgaatc cctgggttct gaccaggcga aagacagcca
     5401 gctgatcggt cagtttggtg ttggtttcta ctctgcgttt atcgtggccg acaaagtgac
     5461 cgtgcgtact cgcgcggcag gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg
     5521 cgaaggtgaa tataccgttg ccgacatcac caaagaagat cgtggtactg aaatcaccct
     5581 gcatctgcgt gaaggcgaag acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag
     5641 caaatactcc gaccatatcg cgctgccggt agagctcgaa aaacgcgaag agaaagacgg
     5701 cgaaaccgtt atctcctggg agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa
     5761 gtcggaaatc accgatgaag agtacaaaga gttctacaaa cacatcgccc acgactttaa
     5821 tgatccgctg acctggagcc acaaccgcgt tgaaggtaag caggagtaca ccagcctgct
     5881 gtacatcccg tcccaggctc cgtgggatat gtggaaccgt gatcataaac acggcctgaa
     5941 actgtacgtc cagcgtgtgt tcatcatgga cgacgcagaa cagttcatgc cgaactatct
     6001 gcgcttcgtg cgtggtctga ttgactccag cgatctgccg ctgaacgttt cccgtgaaat
     6061 cctccaggac agcacggtaa cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca
     6121 aatgctggaa aaactggcga aagacgacgc ggacaaatac cagaccttct ggcaacagtt
     6181 tggcctggta ctgaaagaag gtccggcgga agatttcgct aaccaggaag cgatcgccaa
     6241 actgctgcgt tttgcttcta cttataccga ttcttctgcg cagaccgtgt ctctggaaga
     6301 ctacgtttcc cgcatgaaag aagggcagga gaaaatctac tacatcactg cagacagcta
     6361 tgcggcagcg aagagcagcc cgcacctgga actgctgcgt aagaaaggca tcgaagttct
     6421 gctgctttcc gaccgcatcg atgagtggat gatgaactat ctgactgagt tcgacggtaa
     6481 accgttccag tcggtgtcta aagttgacga gtcgctggaa aaactggctg acgaagttga
     6541 tgagagcgcg aaagaagcgg agaaagcact gactccgttc atcgaccgtg tgaaagccct
     6601 gctcggcgag cgcgtgaaag atgtccgtct gactcaccgt ctgaccgata cgccagcgat
     6661 cgtttcgacc gacgcggacg aaatgagcac tcagatggcg aaactgttcg ctgcggcggg
     6721 ccagaaagtg ccagaagtga aatacatctt cgaactgaac ccggatcacg tactggtgaa
     6781 acgtgcggca gatactgaag atgaagcgaa gttcagcgag tgggtagaac tgctgctgga
     6841 tcaggcgctg ctggcagaac gcggcacgct ggaagatccg aacctgttta ttcgtcgtat
     6901 gaaccagctg ctggtttcct gatgtcatgc cggatgacct ttgtgtcatc cggcattttt
     6961 cttttcatca tctgcacttt ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg
     7021 cctttcttga ggcaatcgcc tgttggtggt atcgtttatc gctttttcaa aaaattcgac
     7081 acattttaag gggattttcg caatgcgtat cattctgctt ggcgctccgg gcgcggggaa
     7141 agggactcag gctcagttca tcatggagaa atatggtatt ccgcaaatct ccactggcga
     7201 tatgctgcgt gctgcggtca aatctggctc cgagctgggt aaacaagcaa aagacattat
     7261 ggatgctggc aaattggtca ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc
     7321 tcaggaagac tgccgtaatg gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc
     7381 agacgcgatg aaagaagcgg gcatcaatgt tgattacgtt ctggaattcg acgtaccgga
     7441 cgaactgatt gttgatcgta tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta
     7501 tcacgttaaa ttcaatccgc cgaaagtaga aggcaaagac gacgttaccg gtgaagaact
     7561 gactacccgt aaagacgatc aggaagaaac cgtgcgtaaa cgtctggttg aataccatca
     7621 gatgactgca ccgctgatcg gctactactc caaagaagcg gaagcgggta acaccaaata
     7681 cgcgaaagtt gacggcacca agccggttgc tgaagttcgc gctgatctgg aaaaaatcct
     7741 cggctaattc gaaagcgcgc acggacagtc ccctcgcccc ctcggggaga gggttagggt
     7801 gaggggaaca ggcccgcaca agcaaactta tcagcaatct caggccggat attcattcgg
     7861 ccttttacaa aaacctcaat ctgcgctatt cctttttctg atttgacctc tcacaacaat
     7921 tagttcttct tcctcacttt tccgctacaa ttatcaacaa gttgaatcga taagaggcgg
     7981 taatgcgtca gactaaaacc ggtatcctgc tggcaaacct gggtacgccc gatgccccca
     8041 cacctgaagc ggtaaaacgc tatctgaaac aatttttaag cgacagacgc gtggttgata
     8101 cctcacggtt gttatggtgg ccgttgctgc gcggcgtgat tttgccgctg cgctcgccgc
     8161 gtgtggcgaa gatgtacgcc tcggtctgga tggaagacgg ctcgccgctg atggtttaca
     8221 gccgccagca acagcaggcg ttggcacaac gtttaccgga tacgcccgta gcgctgggca
     8281 tgagctacgg ctcgccatca ctggaaagcg ccgtcgatga actcctggca gagcatgtag
     8341 atcatattgt ggtgctgccg ctttatccgc aattctcctg ttctacggtc ggtgcggtat
     8401 gggatgaact ggcacgcatt ctggcgcgca aacgtagcat tccggggata tcgtttattc
     8461 gtgattacgc cgataaccac gattacatta atgcactggc gaacagcgta cgcgcttctt
     8521 ttgccaaaca tggcgaaccg gatctgctgc tgctctctta tcatggcatt ccccagcgtt
     8581 atgcagatga aggcgatgat tacccgcaac gttgccgcac aacgactcgc gaactggctt
     8641 ccgcactggg gatggcaccg gaaaaagtga tgatgacctt tcagtcgcgc tttggccggg
     8701 aaccctggct gatgccttat accgacgaaa cgctgaaaat gctcggagaa aaaggcgtag
     8761 gtcatataca ggtgatgtgc ccgggctttg ctgcggattg tctggagacg ctggaagaga
     8821 ttgccgagca aaaccgtgag gtcttcctcg gtgccggcgg gaaaaaatat gaatatattc
     8881 cagcgcttaa tgccacgccg gaacatatcg aaatgatggc taatcttgtt gccgcgtatc
     8941 gctaaagctg agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg gcggttttca
     9001 tcattcgtga ataatgcaaa aaggcatgca gcgtacctgg gtagagtttg aactcacagg
     9061 gctgctgatg cgccgctaac gtctggtaaa gcagacggct gtcatccagc agcggatcga
     9121 actccgcccc ggcaataaaa cagggcggaa cttcgcgagt gagatcatta ttaaacagac
     9181 agtaatacgg tgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca tacatctgca
     9241 aatcctgttg cgttaagcca tcccagacac cgcccaacag acgacgagtc acggaatccc
     9301 gtaatccgta aagcccatac cacagcaaaa cacccgcaat tttaccgcaa tcgatctgtt
     9361 tatcacgcaa ccacaacgca ctggcgagcg ccagcatggc acctgcggaa tcaccggcaa
     9421 agccaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa taacaacaag
     9481 cagccacaat ttcctctatc gcttgcggaa aacgcgcttc aggtgaaagg gggtaatcaa
     9541 taccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga tcgtgggtat
     9601 cgagattgcc gagaataaaa ccgcctccat gcaaataaaa tagcgtcgcc gggctatctg
     9661 gctgcggaca aaagagacgt gtttccacct gcccatattt tgttggaacc atgtaagctc
     9721 tggttgccat ttctggagcg cccgcattcc agaatcggcg ctcaagcgtg taatactgtc
     9781 gttgctcagc aatcgttccc gttgcgggcc aggacggtaa atccggctga agagtattca
     9841 caacggtctt catttcagca gaaataaggt ccagaacagg tagtttgttt tccggcttca
     9901 tacaaaaact cctttcaaat tacgtcattg taaggaacca ctgccatgaa aatgcgatcc
     9961 cgcctgctga tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg tgttaacatt
    10021 cgccgctcag ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa cgtaaatcca
    10081 aacattactt ccctgtaaat gcacgcgatc cgctgcttca gcaattccag ccagaaaacg
    10141 aaaccagcgc cgcctgggtc gtaggtattg accagacgct ggtcgatatt gaagcgaaag
    10201 tggatgatga attcattgag cgttatggat taagcgccgg gcattcactg gtgattgagg
    10261 atgacgtagc cgaagcgctt tatcaggagc taaaacagaa aaacctgatt acccatcagt
    10321 ttgcgggtgg caccattggt aacaccatgc acaactactc ggtgctcgcg gacgaccgtt
    10381 cggtgctgct gggcgtcatg tgcagcaata ttgaaattgg cagttatgcc tatcgttacc
    10441 tgtgtaacac ttccagccgt accgatctta actatctaca aggcgtggat ggcccgattg
    10501 gtcgttgctt tacgctgatt ggcgagtccg gggaacgtac ctttgctatc agtccaggcc
    10561 acatgaacca gctgcgggct gaaagcattc cggaagatgt gattgccgga gcctcggcac
    10621 tggttctcac ctcatatctg gtgcgttgca agccgggtga acccatgccg gaagcaacca
    10681 tgaaagccat tgagtacgcg aagaaatata acgtaccggt ggtgctgacg ctgggcacca
    10741 agtttgtcat tgccgagaat ccgcagtggt ggcagcaatt cctcaaagac cacgtttcta
    10801 tccttgcgat gaacgaagat gaagccgaag cgttgaccgg agaaagcgat ccgttgttgg
    10861 catctgacaa ggcgctggac tgggtagatc tggtgctgtg caccgccggg ccaatcggct
    10921 tgtatatggc gggctttacc gaagacgaag cgaaacgtaa aacccagcat ccgctgctgc
    10981 cgggcgctat agcggaattc aaccagtatg agtttagccg cgccatgcgc cacaaggatt
    11041 gccagaatcc gctgcgtgta tattcgcaca ttgcgccgta catgggcggg ccggaaaaaa
    11101 tcatgaacac taatggagcg ggggatggcg cattggcagc gttgctgcat gacattaccg
    11161 ccaacagcta ccatcgtagc aacgtaccaa actccagcaa acataaattc acctggttaa
    11221 cttattcatc gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat caggtactga
    11281 accagcattc acctcgttta acgcgcggct tgccggagcg tgaagacagc ctggaagagt
    11341 cttactggga tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt cacgccgcat
    11401 ctggcatttg gctctcgatg cctgatgcga cgctggcgcg tcttatcatg cctacatatt
    11461 tttcatattt tacatccggc aaccaccgtt taccccgtca ccacctcacc cgccggtggc
    11521 gtttccagca gttccagcat ggtacgggcg atttcacgct cgcccatcac tacctgattc
    11581 gcaccacgtt cggtgatata cgccacttca tcgtcataat gggcgcgggc aataatctca
    11641 atatccggat ttttcgcgcg ggcagatgcc acaatctcac ccgcttcata accgttggga
    11701 atcgtcagga tcagccattt tgcacattcc agatgcgcca gttgcataat ttcttcgttc
    11761 gccgcattgc ccaatactgc gcggaccccg cgctctcgca gctcatcaac acgggttcgt
    11821 gacgtctcaa tcaccaccag cggaatatca gaggcgagca atttctcccc cagcaggctg
    11881 cctacacgac cgtaacccac cagtagcgca tggttgcaaa tatccactgg gatctgcttc
    11941 tcttcttcga ttgcctcttc cagcgtctgc tcttccagcg tttcggtctt cgccagatat
    12001 ttctccagta gtgcgaacag taccgggttg agcataatcg acaggatcgc ccctgccagt
    12061 actaggtttt gtccggcctg cggcagtaaa ttcaatgcca ttcccagtcc cgccaggata
    12121 aacgcgaact caccaatctg cgccaggctg gcggcgatgg ttaatgccgt acgttgggag
    12181 tgaccaaaca gtcgcaccag gaaaaatgcg gctaacgact taccaaacag aataatcgcc
    12241 agcgtcgcca gcactgccag cggttgctga atcagaatta acggatcaaa caacatcccg
    12301 acggagacaa aaaacagcac cgcaaacgcg tcgcgcaatg gcagcgtatc gtgggcggca
    12361 cggtgactca gttcagactc gttcagtacc atcccggcaa agaacgcacc gagtgcaaag
    12421 gagacatcaa acagctctac cgcaccaaag gcaatcccta acgccagcgc cagcaccgac
    12481 agggtaaaca gctcgcgaga accggttgcc gcgctgcgtg ccataatcca cggcaccaga
    12541 cggcgaccta ccagcatcat aatggcgata aatgcgatca ctttgccgat ggtgatcccc
    12601 atatcgactg caagagtggc aaagcccaca tcgccctgtt ccatcattcc tgccactgcg
    12661 ggcagcaaca ccagcgtcag aaccattacc aggtcttcca caatcaacca accgatggcg
    12721 atttgcccac gctgactgtc aattaattgc cgttcttcaa gtgcgcgcag taacaccacg
    12781 gtactggcgg tggaaagaca taaaccgaac acgataccgg tcattaacga ccagcccagc
    12841 acggcggaga gcgccatacc cagcagcgtc gccacggcta tctgggcgat cgcgccggga
    12901 atggcgatgg actttaccgc catcaaatcc ttgagcgaaa aatgcaaacc gacgccgaac
    12961 atcagcaaaa taacgcccag ttcagccagt tccggcgcaa gcttggtatc ggcaacaaag
    13021 cccggagtga atggtcctgc cagcacaccc gctaacagat atcccaccag aggagaaata
    13081 cgtagtttat tggccagcat gccgaggata aaggcgagca caaggccgcc aacaatggtg
    13141 gtgataagcg gggtggcgtg atgcattccg tctccttttc ctggtggtta ttgtccattt
    13201 ttggccggga aaaccaaaat tacaggtaat agtttatgac aatttcattg atgatgttca
    13261 tgaataatta ttgaattttg cagaaaaatg gaattagctg caaaaaaagc acggatcgga
    13321 aaacagaagg cggtttaaca gaggaaaggc ttatggcgac tgtaaaacga tgcagccaaa
    13381 gtttgccttt ggctgcaatg aaatcagtct ttatgccggt tatcaggcag gaatatggtc
    13441 aacatcccca atagtggcag gaaagcacag attttataga ctaactcgat actggtgtga
    13501 tcggcgataa gccccagaac tgccgctccc agacctccca taccaaaagc aaaaccgaaa
    13561 aagagtccag aaaccatacc gatacgtccc ggaagcagct cctgagcgta aaccagaatg
    13621 gcagagaatg ccgaagcgag gataaatcca ataatcaccg ttaaaacccc cgtccagtgc
    13681 aggctggcgt agggtaaaat cagcgtaaac ggcgcaacgc cgaggataga gccccaaatc
    13741 acatatttcc gcccaatttt atcccctaca ggcccgccga tcaccgtacc tgccgcaacg
    13801 gcaaacagga aggcaaacag atgaagctga gcattctgga tagataatcc gaatttttgc
    13861 atcagataaa aggtgtaata gctgctgatg ctcgccatat agaaatattt cgagaaaatg
    13921 aggattaaca gaatgctgac cgccagtacc actttgttgc gcggcagtgg attgataatc
    13981 gtcgctttgg gttttccttt attcattcgg tgctgtgccg agtaccaacg gctgatttgc
    14041 gccaacacca cgatcgccag cagtgccgca agcacaaacc aggcaacgtt gcctttgcca
    14101 taaggcgcga taatcaccgc cgccagcaag ggtcccaggg aactgccaaa gttgccgccg
    14161 acctgaaaga tagattgcgc caggccatgc cgcccgccgg aagccatacg ggctacgcga
    14221 gaagattccg gatgaaagac cgatgaaccg gtaccgacca gcgccgccgc cagcagaact
    14281 gcgccaaaac tgcccgccag cgcaagcagc accagaccac ttaaggtaaa gcacatgcca
    14341 attggcaacg accacggcat cggatattta tcggtccagt agccgaccac tggttgcagt
    14401 agcgaagagg cgagctggaa ggtgagggtt atcatgccaa tctgcataaa tgtcagagaa
    14461 aattctgact gaagcagcgg ataaatcgcc agaatcagcg attggatcat gtcgttcagc
    14521 agatgtgaga ggctgatagc acctaaaata ccaaacgatg ttcgggcctt ggtcgttgac
    14581 gcagccgcgc ccgccacagg ctggggttgt tcactcattg ccataggaaa gtcacttttt
    14641 cagggttgcg atgtaaagaa tgatcttatt tgtgattatt acccgactaa catacctgta
    14701 tgcgtcgcct gaaggaagtc tcaacgccga atacagaatt tctaatctgg atgcagattt
    14761 atcttcaccg gacgcagact tgtctatgat gtcgcgtcat actatttttc aacacgttga
    14821 aatcaggtca gggagagaag tatgaaatta ttgcagcggg gcgtggcgtt agcgctgtta
    14881 accacattta cactggcgag tgaaactgct ctggcgtatg agcaggataa aacctacaaa
    14941 attacagttc tgcataccaa tgatcatcat gggcattttt ggcgcaatga atatggcgaa
    15001 tatggtctgg cggcgcaaaa aacgctggtg gatggtatcc gcaaagaggt tgcggctgaa
    15061 ggcggtagcg tgctgctact ttccggtggc gacattaaca ctggcgtgcc cgagtctgac
    15121 ttacaggatg ccgaacctga ttttcgcggt atgaatctgg tgggctatga cgcgatggcg
    15181 atcggtaatc atgaatttga taatccgctc accgtattac gccagcagga aaagtgggcc
    15241 aagttcccgt tgctttccgc gaatatctac cagaaaagta ctggcgagcg cctgtttaaa
    15301 ccgtgggcgc tgtttaagcg tcaggatctg aaaattgccg tcattgggct gacaaccgat
    15361 gacacagcaa aaattggtaa cccggaatac ttcactgatg tcgaatttcg taagcccgcc
    15421 gatgaagcga agctggtgat tcaggagctg caacagacag aaaagccaga cattattatc
    15481 gcggcgaccc atatggggca ttacgataat ggtgagcacg gctctaacgc accgggcgat
    15541 gtggagatgg cacgcgcgct gcctgccgga tcgctggcga tgatcgtcgg tggtcactcg
    15601 caagatccgg tctgcatggc ggcagaaaac aaaaaacagg tcgattacgt gccgggtacg
    15661 ccatgcaagc cagatcaaca aaacggcatc tggattgtgc aggcgcatga gtggggcaaa
    15721 tacgtgggac gggctgattt tgagtttcgt aatggcaaaa tgaaaatggt taactaccag
    15781 ctgattccgg tgaacctgaa gaagaaagtg acctgggaag acgggaaaag cgagcgcgtg
    15841 ctttacactc ctgaaatcgc tgaaaaccag caaatgatct cgctgttatc accgttccag
    15901 aacaaaggca aagcgcagct ggaagtgaaa ataggcgaaa ccaatggtcg tctggaaggc
    15961 gatcgtgaca aagtgcgttt tgtacagacc aatatggggc ggttgattct ggcagcccaa
    16021 atggatcgca ctggtgccga ctttgcggtg atgagcggag gcggaattcg tgattctatc
    16081 gaagcaggcg atatcagcta taaaaacgtg ctgaaagtgc agccattcgg caatgtggtg
    16141 gtgtatgccg acatgaccgg taaagaggtg attgattacc tgaccgccgt cgcgcagatg
    16201 aagccagatt caggtgccta cccgcaattt gccaacgtta gctttgtggc gaaagacggc
    16261 agactgaacg accttaaaat caaaggcgaa ccggtcgatc cggcgaaaac ttaccgtatg
    16321 gcgacattaa acttcaatgc caccggcggt gatggatatc cgcgccttga taacaaaccg
    16381 ggctatgtga ataccggctt tattgatgcc gaagtgctga aagcgtatat ccagaaaagc
    16441 tcgccgctgg atgtgagtgt ttatgaaccg aaaggtgagg tgagctggca gtaatccgaa
    16501 agtgccggat gtttgcatcc ggcacaatgc ttaatcgcgg cgggcgatat cagcaaattt
    16561 ggcatcgagg atctttgcca gatcgcctgc cgccagttcg atatccagtc cgcgcttgcc
    16621 gccggaaaca taaatagtgg caaattcttg tgcgggggcg tcgataatcg ttggcagacg
    16681 ttttttctgc cccagtgggc taatcccccc aaccaggtat cccgtcgaac gctgcgcgac
    16741 catcggatcg gccatctcaa ctttcttggc acccagcgct tttgctactt ttttaagatc
    16801 cagttgaccg gcgaccggcg taacggccac ggcaaggtgt ttcatatcac cgttcactgc
    16861 caccagcagc gttttgtaga cctgatccgg attcaaacct aattttttga cgacttcatc
    16921 gccaaaattg gtttcagccg gatcgtgctc gtaggtatgg atttgaaacg aaatcttgtt
    16981 tttttcgagt aatttaactg cgggtgtcat ggtaattttc ctgccttaaa caaaaaaacg
    17041 aagcaagcat acgcctgatg gcggtccaaa aaaaagagtc atcttgccta agagtattgg
    17101 caggatggtg agattgagcg acaatcgagt tacaccgtcg cttaaagtga cggcataata
    17161 ataaaaaaat gaaattcctc tttgacgggc caatagcgat attggccatt tttttagcgc
    17221 aacatttgcg gtaaattccc ttctccatac aggtgtagtg caccgaccgc gaccacatat
    17281 cgccccggcg gcatggcgcg taatttatcc cgccaggcga gatttcgctg atgcatcagc
    17341 acatcgtaca gcgactgact gaacgtattg ggcagcgtta tttcattatt ttgcggcggt
    17401 gcattcagcc accagctcat catttgttgc agcaaccgtg cattggtgtg ccagtgggtc
    17461 agtgtatcgt ccagcagcgc cagtcctttg tcagggagct ggagcaacat ggcaatctgg
    17521 ttttcagccc cttccagttc aatcacgggt ttatgttgtt gcttcgccgc ctgcaatagc
    17581 tggtaatcga taccgtattc tgcccgcagc cccagttttt gtgcctgcgt cgcctgaaga
    17641 accatcgcga tttgccacag cggttgggta gaaaagagtg agggagaaat gcccatctcc
    17701 tggctaatgt gctgcagatt ttggagttgc tcctcgctaa tgcgctcttc cagcgcctcg
    17761 caggcaggca aattggcaaa aggcgtatcg ctggtggaaa catccgcctc gacgatcagc
    17821 gcatcggcgt ttttgagctt tttgagcaaa cgggtgggca ggggagccat atcgtggcta
    17881 cccatatgaa tactgccaat cagatgaaaa tggcgattgc cgggaagggt gatatcgatg
    17941 gcaggccagg tgtaatgatt accgcgcagc gcggcccaaa gcgtttttac ccggtacaac
    18001 agatccatac gacctccctt tgtgaaatat catgctagcg cgcggggagg gatggtgcaa
    18061 atggagttgg ggacttgttt aatgtgtttg tatgattcag tatattcttg tatcgctatt
    18121 cacagggaag caacagttaa aaactatgaa acaggcaaca agaaaaccga cgacacccgg
    18181 agatattctt ctctatgaat atctggtacc gctcgatttg aaaatcaatg agttagcaga
    18241 gttgctgcat gttcatcgta atagcgtcag tgcactgatc aataacaatc gtaaactcac
    18301 cactgagatg gcattccgtc tggcgaaagt ttttgatacc acagtcgatt tttggctaaa
    18361 cctccaggcg gcagttgatc tttgggaagt tgaaaacaac atgcgcaccc aggaagaatt
    18421 gggacggatt gaaacagtgg ctgaatactt ggcacgccgt gaagagcgtg caaaaaaggt
    18481 cgcgtaaggc caaaacatcg cttcatctcg taggccggat aaggcgttta cgccgcatcc
    18541 ggcagtcatg cgtcgatgcc aaatgtgcca ccctaaagca gcgcatccgc aatgacatac
    18601 ttattccttc ggtttaaacc gcagcaaccg gttggcgtta ctcactacgg taatcgacga
    18661 gagcgccatt gccgctccgg caactaccgg gttaagcagt gttccagtaa acggccacaa
    18721 aataccggcg gcgaccggaa taccgatact gttgtagata aaggcaccga gcaggttctg
    18781 cttcatgttg cgcagcgttg cgcgggaaat cgcgagcgca tccgcaacgc ccatcaggct
    18841 atggcgcatc agggtaatcg ccgcggtttc aatggcaaca tcactgccgc cccccatcgc
    18901 aataccgaca tccgcctggg ccagcgctgg cgcatcgtta atgccgtcgc ccaccattgc
    18961 cacctgacgt ccttcacttt gcaggcgttt aatcgcttcg gctttaccgt ccggcagcac
    19021 cccggcgatc acttcatcaa tcccggcttc tttggcgatc gcattggcgg tggttgggtt
    19081 atccccggtc aacatcacca gacgatatcc cgctttatgc aggcgttgca gcgccgccac
    19141 gctatcacta cgcaatggat cgcgtactgc cagcagggct accgctttcc cgtcaaccgc
    19201 cagcagcaca ggcgttgccc cttgcgatgc ctgagcagta atctccgctt cgatagcttt
    19261 ggtaccaacc tgttgctcat ttaacagcgc ctgattgccc agcaataacg catgaccttc
    19321 agcttcaccg ctcacgccca gcccgcgcaa tgtgcggaaa ccgttgacct gcggcagctg
    19381 catatcacct gctttatcga ggatcgcccg tgccagcgga tggctggaac cttgctccag
    19441 tgccgccgcc agacgcaatg cctgcgcttc atcaacatca gcaaacgttt tcactgcgac
    19501 aacctgcggc ttcccttcgg tcagcgtccc ggttttatcg aacacgacgg tatcgagtgt
    19561 actggcgcgt tgcagcgcgt cggcgtcccg caccagcacg ccaaactcag ccgcccgccc
    19621 gacgccggaa ataatcgaca tcggcgtcgc cagccccagc gcacacggac aggcgataat
    19681 cagtaccgtg gtggcaatca ccagggtata aacaatttgc ggtgccggac caaagaagta
    19741 ccagattgcc gcactgacca gcgcaatcac caccaccaca ggtacaaata cggctgagat
    19801 tttatccgcc agctgaccga tttctggctt gctgctctgg gcctggcgca ccatgcgaat
    19861 gatccgtgac agcgtggtat ggctgccaac cgcactggca cgaaacagca cgctaccgtc
    19921 ctgtaccact gtcccggcat ggacgctttc gccttcgcct ttttgctgcg ggattggttc
    19981 gcccgtcagc atcgcttcat ccagccatgc ttcgccctgg gtaatctcgc catctaccgg
    20041 cacgcgatcg ccggtcgtca ggcgcagcaa catacctggc tgcacttccg ccagaggcac
    20101 gcttttttca ccttcgtcag taaccaggcg cgccgtcggt ggggttaaat cgagtaattt
    20161 ctccagcgcc ttagaagaac gctgacgtgc gcgtgcttcc agcatatggc cgagattgat
    20221 cagaccgata atcatcgcgc tggcttcgta ataaagatgt cgcgcttcca tcgggaacca
    20281 ctgcggccac aggttgacgc tcatcgaata gagccacgcc acgccagtac ccagcgccac
    20341 cagcgtatcc atcgtcgctg caccgttcag caggcttttc catgcactgc ggtaaaaatg
    20401 gccgccggcg aaaaccatca ccgccagggt tatcaggccg ataaccaacc acaggctgcg
    20461 gttgtcagcg gtgaccatca tgttatcgcc aatcatcccc cagaccatca ccgggatacc
    20521 caccgccagt gcgacaattg cctgccagcg gaagcgcttc atcgtagcaa cggcggtttc
    20581 ctgctggcgc tcacggcgtt tcgcgtcatc ttcaatcgct tccgcgccgt agcccgcttt
    20641 ttccaccgcc tgcactaaat cttgtgggga ggcactgccc atcaccagcg cagtacgctc
    20701 cgccaggttt acccgtgcct gagtgacgcc cggtacgctt tgcagcgcat tttgtacgcg
    20761 ggtgacacag ctggcgcagc tcatgccgct cagcagcaac tgctggctgt catcgtcatc
    20821 ggcggtcgct gccggaagcg cctcagaaac cgctgtcagt gcttccgacg ggattgatga
    20881 ctccgccagc ggtttagcct ttgggtggct tacagatgcg tcataacccg cttgtttgat
    20941 ggtttcgatc agctgttctg cactggcagt cccggtaacg tgcgcttcag tgatagacac
    21001 atccgcttgt tccacatccg ggcgctgttc gagactttct ttcacgcgtt taacgcagtg
    21061 accgcaggac aggccgtcca gggtcaggtc gataatttgt gacataaaac actcctttaa
    21121 gacagttttg actggctgtg ataaaggtta aaccttccag caaggggaag gtcaagaaat
    21181 taataaacca ggcgagtaaa agtccgtaaa gattaaaaaa ttggctcaat ttgcatcagg
    21241 attagacatt tatctctttg ttttcctgta gttaagttgc gggtgctaag ttaaatcaag
    21301 atgcctgaaa atcggcaccg gggtgaggaa ttacctcccg catctataaa aaggagttaa
    21361 caaaagatgt tagatgcaaa caaattacag caggcagtgg atcaggctta cacccaattt
    21421 cactcactta acggcggaca aaatgccgac tacattccct ttctggcgaa tgtacctagt
    21481 caactggcag cagtggctat tgtgacctgc gatggcaaca tctatcgcgc gggtgatagt
    21541 gattaccgct ttgcgctgga atccatctcg aaagtctgta cgttagccct tgcgttagaa
    21601 gatgtcggcc cgcaggcggt acaggacaaa attggcgctg acccgaccgg attgcccttt
    21661 aactcagtta tcgccttaga gttgcatggc ggcaaaccac tgtcgccact ggtaaatgct
    21721 ggcgctattg ccaccaccag cctgattaac gctgaaaata ttgaacaacg ctggcagcga
    21781 attttacata tccaacagca actggctggt gagcaggtag cgctctctga tgaagtcaac
    21841 cagtcggaac aaacaaccaa cttccataac cgggccattg cctggctgct gtactccgcc
    21901 ggatatctct attgtgatgc aatggaagcc tgtgacgtgt acacccgtca gtgctccacg
    21961 ctcatcaata ctgttgaact ggcaacgctt ggcgcgacgc tggcggcggg tggtgtgaat
    22021 ccgctgacgc ataaacgcgt tcttcaggcc gacaacgtgc cgtacattct ggccgaaatg
    22081 atgatggaag ggctgtatgg tcgctccggt gactgggcgt atcgcgttgg tttaccgggc
    22141 aaaagcggtg taggtggcgg tattctggcg gtcgtccctg gcgtgatggg aattgccgcg
    22201 ttctcaccac cgctggacga agaaggcaac agtgttcgcg gtcaaaaaat ggtggcatcg
    22261 gtcgctaagc aactcggcta taacgtgttt aagggctgat catgatgaac acggaaggta
    22321 ataacggtaa caaacctctc ggtctatgga acgtc
//
LOCUS       DAZFWL020000072        22059 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFWL020000072 DAZFWL020000000
VERSION     DAZFWL020000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.072"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(134..2401)
                     /gene="adiA"
                     /locus_tag="ACHKNP_RS14580"
                     /old_locus_tag="pgaptmp_002916"
     CDS             complement(134..2401)
                     /gene="adiA"
                     /locus_tag="ACHKNP_RS14580"
                     /old_locus_tag="pgaptmp_002916"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HGU4705045.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2600..3508)
                     /gene="melR"
                     /locus_tag="ACHKNP_RS14585"
                     /old_locus_tag="pgaptmp_002917"
     CDS             complement(2600..3508)
                     /gene="melR"
                     /locus_tag="ACHKNP_RS14585"
                     /old_locus_tag="pgaptmp_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HGU4705046.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCKSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRAMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3791..5146
                     /gene="melA"
                     /locus_tag="ACHKNP_RS14590"
                     /old_locus_tag="pgaptmp_002918"
     CDS             3791..5146
                     /gene="melA"
                     /locus_tag="ACHKNP_RS14590"
                     /old_locus_tag="pgaptmp_002918"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGU4705047.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALHDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5249..6670
                     /gene="melB"
                     /locus_tag="ACHKNP_RS14595"
                     /old_locus_tag="pgaptmp_002919"
     CDS             5249..6670
                     /gene="melB"
                     /locus_tag="ACHKNP_RS14595"
                     /old_locus_tag="pgaptmp_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HGU4705048.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6809..7438)
                     /gene="yjdF"
                     /locus_tag="ACHKNP_RS14600"
                     /old_locus_tag="pgaptmp_002920"
     CDS             complement(6809..7438)
                     /gene="yjdF"
                     /locus_tag="ACHKNP_RS14600"
                     /old_locus_tag="pgaptmp_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HGU4705049.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7561..9207)
                     /gene="fumB"
                     /locus_tag="ACHKNP_RS14605"
                     /old_locus_tag="pgaptmp_002921"
     CDS             complement(7561..9207)
                     /gene="fumB"
                     /locus_tag="ACHKNP_RS14605"
                     /old_locus_tag="pgaptmp_002921"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU4705050.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9285..10625)
                     /gene="dcuB"
                     /locus_tag="ACHKNP_RS14610"
                     /old_locus_tag="pgaptmp_002922"
     CDS             complement(9285..10625)
                     /gene="dcuB"
                     /locus_tag="ACHKNP_RS14610"
                     /old_locus_tag="pgaptmp_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HGU4705051.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11196..11915)
                     /gene="dcuR"
                     /locus_tag="ACHKNP_RS14615"
                     /old_locus_tag="pgaptmp_002923"
     CDS             complement(11196..11915)
                     /gene="dcuR"
                     /locus_tag="ACHKNP_RS14615"
                     /old_locus_tag="pgaptmp_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HGU4705052.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11912..13543)
                     /gene="dcuS"
                     /locus_tag="ACHKNP_RS14620"
                     /old_locus_tag="pgaptmp_002924"
     CDS             complement(11912..13543)
                     /gene="dcuS"
                     /locus_tag="ACHKNP_RS14620"
                     /old_locus_tag="pgaptmp_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGU4705053.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13724..13954
                     /gene="yjdI"
                     /locus_tag="ACHKNP_RS14625"
                     /old_locus_tag="pgaptmp_002925"
     CDS             13724..13954
                     /gene="yjdI"
                     /locus_tag="ACHKNP_RS14625"
                     /old_locus_tag="pgaptmp_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HGU4705054.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            13966..14238
                     /gene="yjdJ"
                     /locus_tag="ACHKNP_RS14630"
                     /old_locus_tag="pgaptmp_002926"
     CDS             13966..14238
                     /gene="yjdJ"
                     /locus_tag="ACHKNP_RS14630"
                     /old_locus_tag="pgaptmp_002926"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4705055.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14465..14761
                     /gene="ghoS"
                     /locus_tag="ACHKNP_RS14635"
                     /old_locus_tag="pgaptmp_002927"
     CDS             14465..14761
                     /gene="ghoS"
                     /locus_tag="ACHKNP_RS14635"
                     /old_locus_tag="pgaptmp_002927"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU4705056.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14789..14962
                     /gene="ghoT"
                     /locus_tag="ACHKNP_RS14640"
                     /old_locus_tag="pgaptmp_002928"
     CDS             14789..14962
                     /gene="ghoT"
                     /locus_tag="ACHKNP_RS14640"
                     /old_locus_tag="pgaptmp_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HGU4705057.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15081..16598)
                     /gene="lysS"
                     /locus_tag="ACHKNP_RS14645"
                     /old_locus_tag="pgaptmp_002929"
     CDS             complement(15081..16598)
                     /gene="lysS"
                     /locus_tag="ACHKNP_RS14645"
                     /old_locus_tag="pgaptmp_002929"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU4705058.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QAQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(16835..18292)
                     /gene="dtpC"
                     /locus_tag="ACHKNP_RS14650"
                     /old_locus_tag="pgaptmp_002930"
     CDS             complement(16835..18292)
                     /gene="dtpC"
                     /locus_tag="ACHKNP_RS14650"
                     /old_locus_tag="pgaptmp_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HGU4705059.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMAVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(18351..20498)
                     /gene="cadA"
                     /locus_tag="ACHKNP_RS14655"
                     /old_locus_tag="pgaptmp_002931"
     CDS             complement(18351..20498)
                     /gene="cadA"
                     /locus_tag="ACHKNP_RS14655"
                     /old_locus_tag="pgaptmp_002931"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HGU4705060.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(20578..21912)
                     /gene="cadB"
                     /locus_tag="ACHKNP_RS14660"
                     /old_locus_tag="pgaptmp_002932"
     CDS             complement(20578..21912)
                     /gene="cadB"
                     /locus_tag="ACHKNP_RS14660"
                     /old_locus_tag="pgaptmp_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HGU4705061.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
BASE COUNT         5575 a         5293 c         5531 g         5660 t
ORIGIN      
        1 cggggtgggg ccgcgcaaaa agtatgacat ttttgtttgc agcaattttt atgaaaacgt
       61 tgccatactc aaaaggccta aggccaggcg gcgtaatgtt atttaaacaa ttacgccttc
      121 agcggaatag tggttacgct ttcacgcaca taacgtggta aataccgtca ataatttcag
      181 tcccttcagt ttcgtgttca aatccaggga aatggtggtc ccaggattgc agcgagcgta
      241 aataacttac ttgcggactg tttttatcgc cgaagttttc accagacagc agcatcggga
      301 ttcctggcgg atacgggata actgagtttg ccgcgatgcg tcctggcaga ttttcaatgg
      361 ataccagttc gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg gtgatttccg
      421 ccatcggcag gccggaatag gcttcgttca accgtgcgcc agggttgttt tctttcagcc
      481 aggcaaacat ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt
      541 gttcaacaag ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag tggcgtttga
      601 aggaacaaag ggtgttaacc agagtccccc atttcccacg ggttacgccc atagagaaca
      661 ggaacataat ttggaagtca gtggtgcggg taggcacaat gccgtggcga ccaagccagg
      721 cagtgaccag cgccgccgga acaccggttt cttccagttc accatcttca cccattcccg
      781 gagcaaggat gctgacttta attgggtcga gcatactcca gttatccgga atatctttga
      841 agccgtgcca gctttcgccc ggatgcatta cccagcagtc ctgaacggtg gtcagcagtt
      901 tggttggtgc gtcagcaaag tcataggttt tgccggtttg tgggtcggtg acgacttctt
      961 tgttccacgg tttgaagaac cagctaccgt cagcggtgaa ctctttatat agccgcgcca
     1021 tcgcctgacg gaaatcaacc gcttcgtcaa tcacttcctg tgtcagtgac aggccgctgt
     1081 tgccgtccat catcgacacc gccacgtcgt tggatgcgca gatggcatac agcggggagg
     1141 tggtggcatg catcatgtag gcctggttga agcgggagaa gttaatcgcc ccacgacctt
     1201 cacgtacatg aatataagaa gcctgtgaca gcgcattcag cagtttgtgg gtggagtggg
     1261 tggcgaaaac ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg
     1321 catagatcgg gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc agacgatcgg
     1381 aggttttttc cagcagatcc tgcgcttctt tagcgttata acacacgccg tcataggtgc
     1441 agttggtcac cacgcagtaa gacggttttt gcccggcttt gtctttggtc agcgggcttt
     1501 cactgatttt cttctgcaag gtttcaggtt gcatttcctg cggatagatt ggcccgataa
     1561 tgccgtagcg gttgcggctt ggcaccatat agaccggttt cgcgcctgtc agcatcaagc
     1621 cttgttcgat ggatttatgg caattacggt caacgaccac gacatcgtta tcggtcatgc
     1681 aagcctgcat gatggtgcgg ttagagccgg aagtaccgac gactaccgac caggagcgat
     1741 cggcaccaaa tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca gtatggtcaa
     1801 gcaaagaacc gagggaagtt cgttcgatgc ccatgtcggt gcggaacaga ttttcaccat
     1861 agtagtcatg gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg ccctggtggc
     1921 ctggcgctgc ccaggaatat tcatggatgt cactatattt catcagcgcg ctgaacagtg
     1981 gcggcaacag ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg gcgataaagt
     2041 cggcggtatc ttccagaatc caggcgaatt catcgacaag ctccagcaga tcccggtcca
     2101 ttgcggcgag ggctttttcc cgatcgccca acaggaagac cggcacgttt tgttggcgct
     2161 catgaagctt accgatcaat tgtctgacgt tttgatgttc gtccggatgt tccatttgat
     2221 agctgaacat caggcagtca atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa
     2281 aggaggtgga tttaatcacg gtaacatttt gctggcttaa agcatctgcc agacgctcaa
     2341 cggcgttacc gacccaggtg tcttgatgga gaaactcgct ttcaacaatt aatactttca
     2401 tcattgctta cccggttatg aaggaatctt cgttgcgggc aagtatcttc cggccttttc
     2461 gggctgtaaa gcgcgtgaaa aacaaacatt tttgaaatat ttagttttat aaacaatacg
     2521 ttatgtgatt attttaaatt attttcactg cttattatcg acggctaaac tatttttttg
     2581 gctgatactg atatcgtctt tagccgggaa acgtctggcg gcgctgttgg ctaagtttgc
     2641 ggtattgttg cggcgacatg ccgacatatt tgccgaacgt gctgtaaaaa cgactacttg
     2701 aacgaaagcc tgccgtcagg gcaatatcga gaatactttt atcggtatcg ctcagtaacg
     2761 cgcgaacgtg gttgatgcgc atcgcggtaa tgtactgttt catcgtcaat tgcatggccc
     2821 gctggaatat ccccattgca tagttggcgt taagtttgac gtgctcagcc acatcgttga
     2881 tggtcagcgc ctgatcatag ttttcggcaa taaagcccag catctggcta acataaaatt
     2941 gcgcatggcg cgagacgctg tttttgtgtg tgcgcgaggt tttattgacc agaatcggtt
     3001 cccagccaga gaggctaaat cgcttgagca tcaggccaat ttcatcaatg gcgagctggc
     3061 gaatttgctc gttcggactg tttaattcct gctgccagcg gcgcacttca aacgggctaa
     3121 gttgctgtgt ggccagtgat ttgatcacca tgccgtgagt gacgtggtta atcaggtctt
     3181 tatccagcgg ccaggagaga aacagatgca tcggcagatt aaaaatcgcc atgctcttac
     3241 aggttccggt atctgttagt tggtgcggtg tacaggccca gaacagcgtg atatgaccct
     3301 gattgatatt cactttttca ttgttgatca ggtattccac atcgccatcg aaaggcacat
     3361 tcacttcgac ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg cgaaactcaa
     3421 tctccatccg ctggtattcc gaatacagcg acagcgggct gcgggtctgt ttttcgtcgc
     3481 tgctgcacat aaacgtatct gtattcatgg atggctctct ttcctggaat atcagaatta
     3541 tggcaggagt gagggaggat gactgcgagt gggagcacgg ttttcaccct cttcccagag
     3601 aggcgagggg agcgtccgag catcatgagg ccgaaaactc tgcttttcag gtaacttatt
     3661 accataaact cagatttact gctgcttcac gcaggatctg agtttatggg aatgctcaac
     3721 ctggaagccg gaggttttct gcagattcgc ctgccatgat gaagttattc aagcaagcca
     3781 ggagatctgc atgatgactg cacccaaaat tacatttatc ggcgctggtt cgacgatttt
     3841 cgttaaaaat attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc
     3901 cctgatggac attgatccca cccgcctgga agagtcgcat attgtggtgc gtaagcttat
     3961 ggattcagca ggggccagcg gcaaaatcac ctgccacacc caacagaaag aagccttaca
     4021 tgatgccgat tttgtcgtgg tggcatttca gattggcggt tatgaacctt gcacggtgac
     4081 tgatttcgag gtctgtaagc ggcatggtct ggaacaaacc attgccgata cgttggggcc
     4141 gggcggtatt atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt gcgaggacat
     4201 gacggaagtc tgccccgatg ccaccatgct caactatgtt aacccaatgg cgatgaatac
     4261 ctgggcgatg tatgcccgct atccgcatat caaacaggtc gggctgtgcc attcggtgca
     4321 gggaacggcg gaagagctgg cgcgtgacct caatatcgac ccagctacgc tgcgttaccg
     4381 ttgcgcaggt atcaaccata tggcgtttta cctggagctg gagcgcaaaa ccgccgacgg
     4441 cagttatgtg aatctctacc cggaactgct ggcggcttat gaagcagggc aggcaccgaa
     4501 gccgaatatt catggcaata ctcgctgcca gaatattgtg cgctacgaaa tgttcaaaaa
     4561 gctgggctac ttcgtcacgg aatcgtcaga acattttgct gagtacacac cgtggtttat
     4621 taagccaggt cgtgaggatt tgattgagcg ttataaagta ccgctggatg agtacccgaa
     4681 acgctgcgtc gagcagctgg cgaactggca taaagagctg gaggagtata aaaacgcctc
     4741 ccggattgat attaaaccgt cacgggaata tgccagcaca atcatgaacg ctatctggac
     4801 tggcgagccg agtgtgattt acggcaacgt ccgtaacgat ggtttgattg ataacctgcc
     4861 acaaggatgt tgcgtggaag tagcctgtct ggttgatgct aatggcattc agccgaccaa
     4921 agtcggtacg ctaccttcgc atctggccgc cctgatgcaa accaacatca acgtacagac
     4981 gctgctgacc gaagctattc ttacggaaaa tcgcgaccgt gtttaccacg ccgcgatgat
     5041 ggacccgcat actgctgccg tgctgggcat tgatgaaata tatgctcttg ttgacgacct
     5101 gattgccgcc cacggcgact ggctgccagg ctggttgcac cgttaaaacg cgactaaacg
     5161 ctactgcgcc gggggattta ttccggcgca cacctctgac gataccaata acagaaggcg
     5221 ggcgttggta acagcgaccc gataccctat gagcatttca atgactacaa aactcagtta
     5281 tggatttgga gcgttcggga aggattttgc gatcggcatt gtgtatatgt acctcatgta
     5341 ttactacacc gatgtcgttg ggctgtctgt gggtttggtc ggtactttgt ttctggtggc
     5401 gaggatctgg gatgctatta acgatccgat tatgggatgg attgtaaatg ctacgcgatc
     5461 gcgatggggt aagttcaaac cctggatcct gatcggtacg ttggcaaact ctgtaatctt
     5521 atttctcctc tttagtgcgc atctgtttga aggtactact cagattgtct ttgtttgcgt
     5581 gacctacatc ctctggggca tgacttacac cattatggat attcccttct ggtcgctggt
     5641 tccaaccatc acgctcgata aacgtgagcg cgaacaactg gttccttatc cgcgtttttt
     5701 tgccagtctg gcaggctttg ttacggcagg tgtgacgcta ccatttgtta attatgtcgg
     5761 cggtggcgat cggggatttg gctttcagat gttcactctg gtactaatcg ccttttttat
     5821 tgtttcaacc atcatcactc tgcgcaatgt gcatgaagtc ttttcgtcag acaatcaacc
     5881 gtctgctgaa ggaagccatc tgacacttaa agccatcgtt gcgctaattt ataaaaacga
     5941 tcagctttca tgcctcttgg gtatggctct tgcttataat gtagccagca acattattac
     6001 cggatttgct atctattatt tctcatatgt tatcggtgat gcggatttgt tcccctatta
     6061 tctgtcgtat gcgggagctg ctaacctggt gacgttagta ttcttcccac gcttagttaa
     6121 atcattatcc cgacgcattt tatgggccgg agcatctatt cttccggtgt taagctgtgg
     6181 tgttctcctg ttaatggcat taatgagcta tcacaacgtc gtcctcattg tgattgcggg
     6241 tattttgctg aatgtgggaa cggcgctttt ctgggtatta caggtcatca tggtggcaga
     6301 taccgttgat tacggtgaat ataaactgca cgtacgctgt gaaagtatcg cttactccgt
     6361 gcagactatg gtggtgaagg gcggttcagc ctttgcggct tttttcattg cggttgtgtt
     6421 agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat taggtatgca
     6481 gtttattatg attgctctac caactctgtt tttcatggta acgctgattc tctacttccg
     6541 tttctatcgc ctcaatggcg acacgctgcg caggatccag attcatctgc tggataaata
     6601 tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag tgagtgatgt
     6661 gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt ttatcaggcc
     6721 acgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg aattcacgcc
     6781 gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag cgccgtagct
     6841 ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc agcgcgcaga
     6901 acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca tccgctccct
     6961 ggcccattgc cagcgccgcc caccactcga ttaattcata catcgcgctt atcgccagcg
     7021 ccacgcagca gaccaggaac gccaccattt tacgtccgcg cacgtacatc ccgcgcacga
     7081 gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaaatgcccc agcttgtcat
     7141 acggattacg gctcaacccc agccattcct gcacctcaaa accgatgggg actttcgcgt
     7201 aggtgtattg cccgccgacc atcaggatga tggcgtgaaa gaaaatgagc gtatagagca
     7261 gcggcgttaa cggataacgt ttagcggtgg caagcagtag cggcacgaca ataatcaccg
     7321 gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta taaatcagga
     7381 ttaacgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgta cgggtcatgg
     7441 tgagttcgca agtcagggaa aatactgact attcaccagc aagcgtgaaa ttacaatcgc
     7501 aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg gccgaagagg
     7561 ttacttagtg cagttcgcgc actgtttgtt gacgatttgc tggaagaagt cgttaccttt
     7621 gtcatcgacc aggataaacg ccgggaaatc ttctacttcg attttccaga tagcttccat
     7681 acccagctcc ggataagcga cgcactccag atgcttgatg ctctgctgcg ccagtaccgc
     7741 cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg cgtcggtaac
     7801 ctgctgactg cggttacctt tcgccagcat gatcatgctg ccgccgtggg attgcagcag
     7861 atccacgtag gagtccatac ggcctgcggt ggttgggcca agtgaacctg atggataacc
     7921 ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt actgcggaag
     7981 ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc ggcccacgat
     8041 aatggtgccg gtgagcgaca aacgagtgga taccgggtat tgcgaaagct gggcgaggat
     8101 ctctttcatc gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg cctggcgcag
     8161 ttcttgtgga atgtactggc ctgggttgtg ttccagtttt tcgatccaga taccttcgcg
     8221 gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc cgaccgggca
     8281 ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt atttaccgcc
     8341 aaactgcgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt ccagctggac
     8401 atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt aatagtgagc
     8461 gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa tcacaaacgc
     8521 gatatggtac ggcgggcagg ctgcagtacc gagggtacgc attttctcga cgaggaagtt
     8581 tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatacg ttttgttggc
     8641 agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta ccgcgtacag
     8701 gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt ccagcgccgc
     8761 attctgtgaa tagcgcaggt tatcttcgat ataggtgtta tagacgcctt tcgacagcgc
     8821 ttcttcatca ccgccaccgg tccacacgcg ctggcctttt ttaccgacga tgatcgcggt
     8881 gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggagt ttcttaagaa
     8941 ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa tagccgcaac
     9001 ctgtttctgg tgtgccggac ggagcatgaa agaagcgtcg tgaaaggctt gctgcgccag
     9061 tagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt cggcaacgct
     9121 aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg ggaaaggtgc
     9181 ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta tttgttattc
     9241 gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa gaacccgtac
     9301 atcgcggcga agatccagcc gaagacgcac gatacgctca caccaatcaa ccccggcaga
     9361 ataaaactgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg gtcaaactga
     9421 atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca agccggtgct
     9481 gaagccacga tgtatgccgg atcaacgccg atcgccagcg cgaccggaac aatcgccgcc
     9541 agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat ggcatacgcc
     9601 cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat atgcgcaccg
     9661 aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat cataccggaa
     9721 cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat aataatcagc
     9781 gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg tttgccgccg
     9841 aaggatggac gcaggtccga atcagcacca agaagggcga ctacagcgat tgcaccgagg
     9901 aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatcgagcag cgtcgcggta
     9961 tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa ctcttcgtct
    10021 ttatccagat ctttaccgcg gaaccagctg aagataccga tcgccaggat accgattaac
    10081 gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc atcaaaggtg
    10141 acattaccca gcattgcaac cagagacacg accgcaaccg acaccggact ggcgataatc
    10201 cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat gttgttctta
    10261 atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt accgcaaaga
    10321 atggtcagtg tacaggtcac aaacggcgcg acaattgaga catatttcgg gttgcggcgc
    10381 agcagcttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg caaggtcgcc
    10441 gatgccgcca ccaccgcaat gataaccagc atgacatcaa ccggtggttt acctggctga
    10501 aggtggaaga cgaagaccag aatgaccaga ccgataccgc ctaataaacc cagcgcgata
    10561 ccaccctttc tggcaccata aaacagacat atcagtatta tgataagttg gatagtaaat
    10621 aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga tcacagtcac
    10681 gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg tcgttaccgg
    10741 ctttagcaaa tacctcacag taaatattgg ctgattaaag cagcggcaaa tttatgcact
    10801 gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata gtttgtactg
    10861 cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc tattttttgt
    10921 tctcagggca gatatcatta ggtttaaaga ttaatctgat ctacccattt gtgggtaaaa
    10981 atacacataa tgcgggtgac ataatagtta attaactttt gttatcgttt tgaaattaaa
    11041 aacaccgttc acctgaagag atattaattt ttagcgatga tggagggata attatatttg
    11101 atctggcaca agttttactg atgaaggatg taacttgtgc caggggtatt ttgcattacg
    11161 gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg tttcagtagt
    11221 gagtagtgtt ctgcctgaat acggtaacgg taaaccggac gccccgtgac gccataatgg
    11281 atactggtga acaagatgtg gcagttgacc agccagatga ggtatttacg gcaggaaaca
    11341 cgcgagatgt taacctcgtt agccagctcg tcggttgaaa actcatagtc ctgatgcgcg
    11401 tcaatccact ggcacagcgt gcgtaaagtc tgcggcgtta agccttttgg caagcgacga
    11461 ggatcctgtt cgttggagct gctgccgtgg attagctgat caagctcggc ctggtcataa
    11521 tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc ctcttcaaag
    11581 cgggaagcct ggaagggttt gatcaggtaa tccacgacac cgtaatgcag cgaatcttta
    11641 atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca acgcgcgtta
    11701 tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc gagcaatatc
    11761 aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag cgtcgaggct
    11821 gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag ctccgcgacc
    11881 attgcgtcgt catcgataat taatacattg atcatctgtt cgacctctcc ccgtcccagg
    11941 gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg atgctgccgc
    12001 cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg cttccttttg
    12061 tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgattccc ggtccatcat
    12121 cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta atttcgccac
    12181 cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat gtggtaatca
    12241 gcgtcgcgac ctggtcctca ctgccgctgt ctggcagctg gctttcactg tttaaaatca
    12301 gcgtatggcc taaatcggtc gcgcggttaa tcttgctgat taaaaaacca gcgataaccg
    12361 gagatttgat cttacccagc agagagccaa tctcttcctg atagttattg gctgttttga
    12421 gaatgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga atcacatgca
    12481 atttattcat aaattcgtgg gatcgttcac gaagtgcatc agcatagttg accagaccgt
    12541 cgagtcgctg catcagttta cgtacttcag ttttgtccct gaaggttgaa atggcaccga
    12601 tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccgg tctttaatcg
    12661 taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca tctaccacct
    12721 gtgaccatga gtggctgagc gtcgacagtt tctcatcgtc ctgcgactta cggtaattca
    12781 gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc acggcaacga
    12841 cgccttcttt gatagactgc aacatggcct ggcgctgctc aaacagcgtg gagatttcgt
    12901 agggttccag gccgaaaagg atttttttca gtaccttaac cagaatgcag gtgccaatca
    12961 gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg tcattgattt
    13021 gttgggtcac gcggcttaac tcaaggccga tcgccaccac gccaatttgt ttatgatttt
    13081 catcgtagat gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga ttgatagcga
    13141 cattttcttt gccattcagc gctttaagga tgtcatcacc tttaaatggc tgaccaatac
    13201 gctgggcttc aggatgcgag tagcgaagac tttgcatatc ggtaacgaca ataaacagca
    13261 gatcgttgcg tttgcgcacg gcttccgcga tggcctggat tccgctctcc tgcggttttt
    13321 tctgcaagcc ctgacggatt tccggcgagt cggcgagggt acgcgccact gccagtgcct
    13381 tgttggccag cccgtctcgc gtcatatcac tgatttgcga gaagtaaatc agatgcacca
    13441 ccaatagcac cgagaacagt accgcgctga ccattaagat cactgtggta ctcaatttca
    13501 tcggacgttt gcgtaacatg cggtagggca atgaatgtct catcagcttc cttgtgtgac
    13561 aaatttctta agcattatct ctgatgaggc gggtaattca aagggagtaa gaatgattgg
    13621 ctatataggg gaagagactc tggcaacgga aactgccagt gctgtatgaa gattccaggg
    13681 ctatgcttat agcgataatc atactgatga gagagggaaa gtcatggatc aggcgctact
    13741 ggacgagggt tatcgctgtt ataccggcga aaagatcgat gtctatttca acactgcgat
    13801 atgtcagcat tctggcaatt gcgtacgtgg caacggcaag ttatttaatc tcaaacgaaa
    13861 gccgtggatc atgccggatg aagtcgacgt cgtcactgtg gttaaagtga ttgatacgtg
    13921 cccgagcggc gcgctgaaat accgtcataa ataagcgagg gtaaaatgga aatacgcgaa
    13981 ggccacaata aattttacat taatgacgaa caaggcaagc aaatcgctga aattgtcttt
    14041 gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga aagcctgaaa
    14101 gggcagggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg tcgggaaaaa
    14161 cgaaaaatta tcccactatg cccatttgcg aaacacgaat ttgataaaac gcgggagtat
    14221 gatgatattc gcagttgatg ggagagtaca gagtcacgat atttttcatg ctctccgcgg
    14281 agtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatgggc agagagcgcg
    14341 tttttttgtc ccaagtcatc ccctttactg agcaaaaaaa agaatatctc ctatatgaga
    14401 atcatcaatc ggggttaata atttttgcgt ccccagagca ttcaatattg ataggagtca
    14461 tattatggaa ggtaaaaaca agttcaatac ttatgttgtt tcttttgatt atccatcatc
    14521 ttattcctca gtgttcttaa ggttaagatc attgatgtat gatatgaatt tctcttctat
    14581 cgtggctgat gaatatggga taccacgaca attgaatgaa aattccttcg caataacgac
    14641 atcgttagcc gcaagtgaaa tcgaagattt aatcaggctt aaatgcttag acttaccgga
    14701 tattgatttt gacctcaaca ttatgacagt tgatgactat ttccgtcagt tttacaagta
    14761 gccaggaagg ataagaagaa cataaaaaat ggcactattc tctaaaatat taatttttta
    14821 tgtgattggt gtgaacatat cctttgtcat tatctggttt atctcacatg agaaaacaca
    14881 tattcgttta cttagtgcat tcctggtcgg aataacctgg ccaatgagtc tgcctgtggc
    14941 attacttttt tctctctttt aggagctgta gttgagtgga cccgctggtt atcgtgaaac
    15001 gaagcgtctc tcctcaaaac tggatttgcg gaacgacgaa cgaagcagtc gcctgactgc
    15061 ttcgttcatt aaagtgaaat ttatttctgt gggcgcatcg ccgggaagag aataacgtcg
    15121 cgaatagtat gactgtttgt aaacagcata atcattcggt cgataccaat acccagacca
    15181 gcggttggcg gcagaccata ttccagcgca gtcacgtaat cttcgtcata gaacatggct
    15241 tcgtcgtcac ctgcagcttt agcgttaacc tgttcctgga aacgttcagc ctgatcttct
    15301 gcgtcgttta attcgctaaa accattaccg atttcacgac caccgatgaa gaattcaaaa
    15361 cggtcggtga tttctgggtt aacatcatta cggcgtgcca gcggtgacac ttctgccgga
    15421 tattccgtaa taaaggttgg ctgaatcaga tgtgcttctg ccacttcatc aaagatctcc
    15481 gtgacaatac gtcccagccc ccagcttttc tctaccgtaa taccgataga ttcagctaat
    15541 gctttagcag catcaaaatt atccaggtcg gccatatcgg tttctggacg atattttttg
    15601 attgcttcgc gcatggtgag tttttcaaac ggtttgccga aatcaaacac atgctcgcca
    15661 taagtgactt tagtggtacc cagaacctct tgtgccaggg tgcggaacag ggactctgtc
    15721 agttcaatca aatcgtggta atccgcatac gccatgtaga gttccatcat tgtgaactca
    15781 ggattatggc gaacagaaat accttcatta cggaagttac ggttgatttc gaatacccgt
    15841 tcaaaaccgc ctacaaccag acgtttcaga tacagctccg gcgcgatacg caggtacata
    15901 tctaaatcca gagcgttatg atgggtaata aacgggcgag cagatgcccc acctggaatt
    15961 acctgcatca tcggggtttc tacttccata aagccgcgcg cgaccatgaa ctggcggata
    16021 gcggccagaa tttttgaacg gacaacaaac gtttgacggg atttatcgtt agcgatgagg
    16081 tccagataac gttgacgata acggacttcc tgagcctgca gaccatggaa tttatctggt
    16141 aaaggacgta gtgcttttgt cagcaggcgc aactcagtac agtgaatgga aagctcaccg
    16201 gtttgcgtct taaacagcgt accgcgggca ccgataatgt cacccagatc ccatttttta
    16261 aactgatcgt tataaacacc ttctggcagg ctatctcttg caacgtacag ttgaatacgg
    16321 ccaccgacat cctgcaacgt tacaaaggag gctttcccca tgatacgacg ggtcatcatt
    16381 cggccagcaa ccgagacttc aatgtttaag gattccagtt cctggttatc cttcgcatca
    16441 aactcttcgt gcagctggtc agaggtatgg tcgcggcgaa aatcattggg aaacgccaca
    16501 ccttgctgac gtagtgccgc cagtttttcg cggcgatttc tcagttcatc gttaaaatca
    16561 atagcctcat tggctccccg tgtttcttgt tcagacattt tggttcctct aaatccagct
    16621 ttcaatttag cgtagataaa gagacagatc ggtacactgt attatctgcc tcgactatta
    16681 ataactcagt ttatcttatc gaaactaacg cttacctcaa gttgatatca ctaataaata
    16741 accatcaaaa tggtttaaat gacttattaa tctagatttg ctagtgttat aaactgaaag
    16801 ttaatttatt cataaccaat tgtttttacg acagttaatc gttgctctcc tgtatcatat
    16861 tcgtcggcgt gctaaacaga aaacgggtag caaaggccaa tacgacgatg atcgcgacac
    16921 aagccaacgt ccactctccc atctgagaaa agaatcgctg gtaagctgca attgccatac
    16981 cgctaatttg cgactctgtc gtctgctgtg ccacgacgcc tgccagccag ttggcgaccg
    17041 cgcctgttgc cagcatataa ataccggtta atacgccaga cattttcaga cgcgtgattt
    17101 gcgcaatcgc caccgggtca ataaagagtt cggcaaagcc cattagcgcc agcccggata
    17161 tcatcacggc cattgacgct tgaccgccag ccgctgcatg tcgggcatca aatgccaaca
    17221 acataaagcc acaagccatc agcagtaagc caaaggcaaa cttcagccag acgcgcaatg
    17281 ttgagttgcc gcggctttct ggcgacgcca gccaggccag tacaacccca gcgagcatca
    17341 ctgcaattgc attcaccgac tggaatagtg ctgtaggtac ttcaatattg aatgcctgac
    17401 gattcacaaa gcgatcgata aacaagctga tggtactgcc gccctgttgt gccagtaccc
    17461 agaacaacgt cccgacaaac atcaacaata caatttgcca aagagcacgg cggtgttcgg
    17521 ggaatttgat catcatgcgg gcaatgattt gtgcggcaat gaggcaaacg atcgccagca
    17581 aatatccgga ccagtcgttc tccagcagca gggtaaaaaa tactggggct aaacagagca
    17641 tcaccactaa ccagctccat actggtaagg caaatttcac gctagtgagc gcttttttat
    17701 ccatactacg cgtggactgg aaatgacgat gcccgcttaa gaaaatcaac aaaccgataa
    17761 acatgccgcc acccgcaagg gcaaagccaa catgccatcc ataccactga gcagccaggc
    17821 cgcaggcgat tggggcggcg atagaaccga tattgcccgc agcatacagc agcgaaaaac
    17881 cgccatcacg tcgatgatcg ttctcgtcgt agagctcgcc aagcaaacag ctgatgtttg
    17941 atttgaataa accgtagcca caaataatga ttgccagcgc cagatacagg ctaaaggttg
    18001 aatttgtatc aatgcccagc accacatggc caagggtcat taacagcgcg ccggcaatca
    18061 ctgcagtgcg gttgccgagc aggcggtcgg caagccagcc gccgagaata ggggtaacgt
    18121 aaaccagaga agcatatgcg ctgaacaggc tgatggcatg gttatcatca aaaccaagct
    18181 gatgggtgag atagagaatg agtaaggcac gcatgccgta aaaactgaag tactcccaga
    18241 tttggatcgc cacgatatag tatatcgcgc gcggctgtga gggtgttttc atgtgttctc
    18301 cttatgagca aaaaagggaa gtggcaagcc acttcccttg tacgagctaa ttattttttg
    18361 ctttcttctt tcaatacctt aacggtatag cggccatcag cctgacggta tgcaccgtga
    18421 atatcggttt caaagcccgg atagtgagcg ccgatttcac acagcatctg caggaactcc
    18481 agaaccggac ggctttcttc ggtgatcatt tcacccggca ttaccagagg aactcccggc
    18541 ggatacggaa ggatcatatt ggcgttaata cgacctacca tttcgtcgag gtaaacttct
    18601 tcggtcatac cgtgcagctc tttctggaat gcagcatacg gagtcattac catcgtcggc
    18661 agcacttcaa atgcgcggta catcagatcc ggcagattgt ggtgaacaat cagtttgtgg
    18721 atattctgag ccagttcctg aatacgcatg ttttcataga attcaggatc ttcacgatac
    18781 agagacggca gcatgttttt cacacgcagg ttcaggtcga acgcacgttt gaagtcagtc
    18841 agagcacgca gcaggctcag tgctttggtc ttatcgatac cgatgctgaa caggaacagc
    18901 aggttatacg gaccggtttt ctcaacaacg atgccatgtt cgtcgaggta tttcgccacg
    18961 atgctggccg gaataccaaa gtcgctcatg gtgccgtctt tttccatccc cggagtcagc
    19021 agggtgactt tgatcgggtc aagatacatg tgctcgttat cgatgttttt gaagccgtgc
    19081 caggtgctgt cagaacgcag cggccagcat tcagtcgtat cgatatgatc cggctgccat
    19141 acatcaaaga accagccatc agattccgtt ctcagacgtt tgatctcttt acggaatttg
    19201 atcgcacgtt caatggaacc gttgatcaga cgtttacctg cattgccttt catcatcgcc
    19261 gcagcggttt cagtggacgc cacgataccg tagtgcggag aagtggtggt gtgcatcatg
    19321 taggcttcgt taaaggtttc ttcgtttacg tcacctttaa cgtggatcat ggaagcctga
    19381 gagaacgccg ccagcagttt gtgagtggac tgggtttcgt aaatcacttt cccttctaca
    19441 cggccaccgc tcataccgca tttaccttcg taaatcggtg agaagttggt gtaaggcacc
    19501 cacgcggagt caaagtggat ggatttcaca tccagtgttt tcttgatgaa gtcggtgttg
    19561 tacagcagac catcataggt agagttggta attacagcat gtaccggcca ggttgcgttt
    19621 ggtgtttctt tcacgcgctt agcaatggta gcgtgctgga attcactctg tgggatacca
    19681 ccaagaatac cgtaagcgtt acgggtcggg cggaaataga ttggcgtaac atcgctcatc
    19741 atcatcaggt gggtcagcga tttgtggcag ttacggtcaa tcagaatggt gctgcctgcc
    19801 ggagcagagt acatgccaac aattttgttc gcagtggaag taccgttggt caccatgtag
    19861 ctgcggtctg cgttaaagac gcgagcgata tactgttctg cttctttgtg tggaccactg
    19921 tgatccagca gagaacccag ttcagatact gaaatggaaa tatcagattt catggtattc
    19981 ggaccaaaga aatcatagaa caggctacct accgggcttt tctggaatgc agtaccgccc
    20041 atgtgaccag gagtacagaa agtatattta ccttcacgaa catatttaaa cagtgcttta
    20101 gtcagcggag gcagaatagt gttgatatat tcgtcagtgg tctgcttgat cttattagca
    20161 atatcttcag cagcacccag cgcatattca aagaagctaa tctgtaaacg caggtcattc
    20221 aggcttacat cgagagtgga atacgtatta gcgaacgcgt acaacggcag gttctcgttc
    20281 attttgctaa tttcttcgca cagctcgaga ttatatttat cccagtcaaa aataacgccg
    20341 cacagacgcg cattgttttc gatcagtttt aataagtcgt cacggtcgtt cgggtaaaca
    20401 atctggaagt tcagacgttc aagcgcgcga tgaagttcac ggatgggttc ttctttaaaa
    20461 taaaccccca tgtgattcaa tattgcaata acgttcatag tcatatctcc aggtaaaaaa
    20521 ggcccctccc aacacatggg acaaaatgaa aggaggagcc tcggaaaata cttttaatta
    20581 atgtgcgtta gacgcggtgt ggttatccat tgagtggctc tggcgctcgt gcattttgcg
    20641 agcgtagaac atcaggataa tcaggctgac gatgaaggta cctgccagct cgaaggagct
    20701 tgcgcccatc agcgcgatga agcagaacac acaacccagt acagagcaga tcaggctgac
    20761 aaagttgcgg atgttaacgc cttcaaaacg aatcaggtca acgcaagagt agaagtacgg
    20821 cagcatagtc agcagtactg cgataccggt cagttcaccg aacaggtcag atgctttacc
    20881 accggcagag ttcatcagag tgatgaggat catcagggca gtcattttca ctgcagccag
    20941 cagcagacct tttttcggaa taccgttgct gtcgacttca ccataaactt tcgggaagtt
    21001 accgtcgtta gcggcacgta cacctgcctg gcctaccaac atcatccagg agcccagaga
    21061 agtcaggcac gcaaaggcgg tgaatgcaga aaccagcggc gcagcccagt taccgaggat
    21121 ggttgaagca ctgattgcaa acggagcacc ggaagctgcc attacagaag atggatacat
    21181 accggaaagc acctgagtcg cagcgatgta aacaatacct gctaaaccag tacccagcat
    21241 ggttgccagc ggaacggtac gtttcgggtt tttaaccata ccagtactta ctgctgcgga
    21301 ttcaacaccc acgaaggccc acaggcagag cagaatactt ttaatgatcg catgaccatc
    21361 agtggtatcc gcagtattcc agttagctgc ataagttgcc gcatcaaacc aatgccagcc
    21421 aacaatagcg gtcattacta ccggaataag aaccagcacc agaccaatag tggttaaacg
    21481 gcttacccaa gtaccgccga gcatatttac aaaggtaaat acccagacga tagcaataca
    21541 ggcgataccc gccggaacag gatcatttaa tactgggaag aaggtggaaa gataagatac
    21601 cgcggtaata ccaatcgcca ggttaccaat ccagttagca tggtaataaa gaacacctgt
    21661 ctgaaaacca aatgcagggg aaatttctcc ggcataagca attgggccac cttgttgcgg
    21721 gttttttgtt gccagtcggg catatacata cgccagcgac attgcaccaa taatagagat
    21781 aatccaaccc cagatagcaa taccaccgat acttgctagg ttcgcaggta ataatgcaat
    21841 accgctcccc atcatattac cggcaacaac accggtacag gcaaatagcc cgatcttctt
    21901 ggcagaactc atgctcttct cctaatttca tttttgaatt tggagtccgg gtcatgatgt
    21961 ataactattt cctgaccaga ccaaactggc gctaagaata ctcacgaaaa aaggattaat
    22021 cctaaagatt aggtgaaata acacaaaagt ttctgtaag
//
LOCUS       DAZFWL020000073        21994 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFWL020000073 DAZFWL020000000
VERSION     DAZFWL020000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.073"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..670)
                     /locus_tag="ACHKNP_RS14665"
                     /old_locus_tag="pgaptmp_002933"
     CDS             complement(<1..670)
                     /locus_tag="ACHKNP_RS14665"
                     /old_locus_tag="pgaptmp_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HGU4705062.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWVLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFH"
     gene            complement(873..1274)
                     /gene="nikR"
                     /locus_tag="ACHKNP_RS14670"
                     /old_locus_tag="pgaptmp_002934"
     CDS             complement(873..1274)
                     /gene="nikR"
                     /locus_tag="ACHKNP_RS14670"
                     /old_locus_tag="pgaptmp_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HGU4705063.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1280..2086)
                     /gene="nikE"
                     /locus_tag="ACHKNP_RS14675"
                     /old_locus_tag="pgaptmp_002935"
     CDS             complement(1280..2086)
                     /gene="nikE"
                     /locus_tag="ACHKNP_RS14675"
                     /old_locus_tag="pgaptmp_002935"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HGU4705064.1"
                     /translation="MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(2083..2847)
                     /gene="nikD"
                     /locus_tag="ACHKNP_RS14680"
                     /old_locus_tag="pgaptmp_002936"
     CDS             complement(2083..2847)
                     /gene="nikD"
                     /locus_tag="ACHKNP_RS14680"
                     /old_locus_tag="pgaptmp_002936"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HGU4705065.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2847..3680)
                     /gene="nikC"
                     /locus_tag="ACHKNP_RS14685"
                     /old_locus_tag="pgaptmp_002937"
     CDS             complement(2847..3680)
                     /gene="nikC"
                     /locus_tag="ACHKNP_RS14685"
                     /old_locus_tag="pgaptmp_002937"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HGU4705066.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMTATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3677..4621)
                     /gene="nikB"
                     /locus_tag="ACHKNP_RS14690"
                     /old_locus_tag="pgaptmp_002938"
     CDS             complement(3677..4621)
                     /gene="nikB"
                     /locus_tag="ACHKNP_RS14690"
                     /old_locus_tag="pgaptmp_002938"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HGU4705067.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4621..6195)
                     /gene="nikA"
                     /locus_tag="ACHKNP_RS14695"
                     /old_locus_tag="pgaptmp_002939"
     CDS             complement(4621..6195)
                     /gene="nikA"
                     /locus_tag="ACHKNP_RS14695"
                     /old_locus_tag="pgaptmp_002939"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4705068.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6306..6893)
                     /gene="acpT"
                     /locus_tag="ACHKNP_RS14700"
                     /old_locus_tag="pgaptmp_002940"
     CDS             complement(6306..6893)
                     /gene="acpT"
                     /locus_tag="ACHKNP_RS14700"
                     /old_locus_tag="pgaptmp_002940"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HGU4705069.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWH
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6948..7997)
                     /locus_tag="ACHKNP_RS14705"
                     /old_locus_tag="pgaptmp_002941"
     CDS             complement(6948..7997)
                     /locus_tag="ACHKNP_RS14705"
                     /old_locus_tag="pgaptmp_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU4705070.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            8129..9346
                     /locus_tag="ACHKNP_RS14710"
                     /old_locus_tag="pgaptmp_002942"
     CDS             8129..9346
                     /locus_tag="ACHKNP_RS14710"
                     /old_locus_tag="pgaptmp_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4705071.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9350..9907)
                     /gene="dcrB"
                     /locus_tag="ACHKNP_RS14715"
                     /old_locus_tag="pgaptmp_002943"
     CDS             complement(9350..9907)
                     /gene="dcrB"
                     /locus_tag="ACHKNP_RS14715"
                     /old_locus_tag="pgaptmp_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HGU4705072.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9980..10645)
                     /locus_tag="ACHKNP_RS14720"
                     /old_locus_tag="pgaptmp_002944"
     CDS             complement(9980..10645)
                     /locus_tag="ACHKNP_RS14720"
                     /old_locus_tag="pgaptmp_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HGU4705073.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10866..11111
                     /gene="tusA"
                     /locus_tag="ACHKNP_RS14725"
                     /old_locus_tag="pgaptmp_002945"
     CDS             10866..11111
                     /gene="tusA"
                     /locus_tag="ACHKNP_RS14725"
                     /old_locus_tag="pgaptmp_002945"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HGU4705074.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(11213..13411)
                     /gene="zntA"
                     /locus_tag="ACHKNP_RS14730"
                     /old_locus_tag="pgaptmp_002946"
     CDS             complement(11213..13411)
                     /gene="zntA"
                     /locus_tag="ACHKNP_RS14730"
                     /old_locus_tag="pgaptmp_002946"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HGU4705075.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEEKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13485..14111)
                     /locus_tag="ACHKNP_RS14735"
                     /old_locus_tag="pgaptmp_002947"
     CDS             complement(13485..14111)
                     /locus_tag="ACHKNP_RS14735"
                     /old_locus_tag="pgaptmp_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HGU4705076.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14252..14611
                     /locus_tag="ACHKNP_RS14740"
                     /old_locus_tag="pgaptmp_002948"
     CDS             14252..14611
                     /locus_tag="ACHKNP_RS14740"
                     /old_locus_tag="pgaptmp_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HGU4705077.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14614..14883)
                     /locus_tag="ACHKNP_RS14745"
                     /old_locus_tag="pgaptmp_002949"
     CDS             complement(14614..14883)
                     /locus_tag="ACHKNP_RS14745"
                     /old_locus_tag="pgaptmp_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HGU4705078.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14873..15469)
                     /gene="rsmD"
                     /locus_tag="ACHKNP_RS14750"
                     /old_locus_tag="pgaptmp_002950"
     CDS             complement(14873..15469)
                     /gene="rsmD"
                     /locus_tag="ACHKNP_RS14750"
                     /old_locus_tag="pgaptmp_002950"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HGU4705079.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15619..17115
                     /gene="ftsY"
                     /locus_tag="ACHKNP_RS14755"
                     /old_locus_tag="pgaptmp_002951"
     CDS             15619..17115
                     /gene="ftsY"
                     /locus_tag="ACHKNP_RS14755"
                     /old_locus_tag="pgaptmp_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGU4705080.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            17118..17786
                     /gene="ftsE"
                     /locus_tag="ACHKNP_RS14760"
                     /old_locus_tag="pgaptmp_002952"
     CDS             17118..17786
                     /gene="ftsE"
                     /locus_tag="ACHKNP_RS14760"
                     /old_locus_tag="pgaptmp_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGU4705081.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17779..18837
                     /gene="ftsX"
                     /locus_tag="ACHKNP_RS14765"
                     /old_locus_tag="pgaptmp_002953"
     CDS             17779..18837
                     /gene="ftsX"
                     /locus_tag="ACHKNP_RS14765"
                     /old_locus_tag="pgaptmp_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGU4705082.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILMLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            19082..19936
                     /gene="rpoH"
                     /locus_tag="ACHKNP_RS14770"
                     /old_locus_tag="pgaptmp_002954"
     CDS             19082..19936
                     /gene="rpoH"
                     /locus_tag="ACHKNP_RS14770"
                     /old_locus_tag="pgaptmp_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HGU4705083.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            20207..21310
                     /gene="livJ"
                     /locus_tag="ACHKNP_RS14775"
                     /old_locus_tag="pgaptmp_002955"
     CDS             20207..21310
                     /gene="livJ"
                     /locus_tag="ACHKNP_RS14775"
                     /old_locus_tag="pgaptmp_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HGU4705084.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21509..21892)
                     /gene="panM"
                     /locus_tag="ACHKNP_RS14780"
                     /old_locus_tag="pgaptmp_002956"
     CDS             complement(21509..21892)
                     /gene="panM"
                     /locus_tag="ACHKNP_RS14780"
                     /old_locus_tag="pgaptmp_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HGU4705085.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGL
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5202 a         5831 c         6098 g         4863 t
ORIGIN      
        1 ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc cggtacggcg
       61 gcagcggcgc aggcagcacc tcatccgctt ccggcacccg ctcacaccag ccgagtaccc
      121 accacggccc ctgcggactg tttgtcgcca gataacgatg cggactcagg cgaagctctt
      181 ccggcagcgc ctgccagagt gcggccagcc ggtgaccttc atccagtttc gccacaccgc
      241 cgcgcaccag ccagagtgat tcgctgcggc tgtaaccgtc ctcaccggga aacaggtgct
      301 caaaatacag gcttctgccg ccgttatcac tgagtatcag tgtgttatcg cgcagctgta
      361 agcggatatc cgcaggcatt ttccagccgg ggccgaggct ccccaccggc gcgggcgttt
      421 ttgtccggta actgctgtag gtgcgggaga gaatgaacgg cagcgggccg ggcagggcga
      481 tgtcggtttc accgggaagg acctttgcac ccagcagcgg attgaccgga tggccggacg
      541 tcaccccgcc ggggcacacc gaacaggcca cgccggtggg ggcaccaata cgtaccccgg
      601 ctgaaccctg aacaatgcta ccgccatact gcgtcatgtc accctgacgc gccgccggtt
      661 ttccgctcat cgtaaaatcc ttttctatta ttttaatgac agtatcttca tacgaaaaag
      721 cgaagaaaaa cttgtggtca caatgaattg aagattttta ggaatatatt tagaacgtta
      781 caaaagagaa ggttcggata gggtatttag agaggaatca ggtgtgctag tggccctttg
      841 ttgatcgttc accaaaggac ccaataatag actcaatctt ccttcggcaa gcactgcaaa
      901 tgcccgtgcc gcacgccgcg ctgggcgata acgtcatcgg caaaatgctg cacgtcgccc
      961 atatcacctt tcagcacggc gatttccaga cagtcgtcgt gattgatatg cacatgcagc
     1021 gtggcgacgg agagatcgtg gtgatggtgc tgggtggaga caatgcggct ggctaagtcg
     1081 cgtttttcgt gttcatacac ataggagagc accgcgaaac cttgcgtgcc gtgctgctga
     1141 gtggcctcct gggccagagc gctacgcaga atatcgcgga tagcttcgga acgattgtta
     1201 taaccacgac gctggctcag gctgtccagc gtctccagta aatcgtcatc aagcgtgatg
     1261 gtgactcgtt gcatttgcgt taaacctttt ctgtggtgcg acggcgcacg gggaatgcgg
     1321 gtaataccgc gttttgtagc acacgtccgg cgtcagagga aaaggttaat ttatctccca
     1381 ccacctgggt ttcgacgatt tgtccgttgt ccatcaccat cacccgctga cagaagcgtt
     1441 ccaccaggcg taagtcgtgg gtgatgaaca ggcaggcggt gccaaactgt tgttgtagct
     1501 ttttcagcag gcgaatgacg cccgcctgta acacgagatc aaggttagaa acggcttcat
     1561 ccagaatcag tagtttcggt tcgaccgcca gcgcgcgagc caggcagacg cgctggagct
     1621 ggccgccgct taactgcggt gggcgtttgt cgagaacgct gtcatcgaga tcgaccgcct
     1681 tcagcatttc gctggcgcgc gccagttgtt cggatttttt cagtgatagc aggtggcgca
     1741 tcggttcacg caggatctcg cgcacggttt tgcgcggatt cacggcgctg atggagtcct
     1801 gaaataccat ctgaatatcg cggcggaacg ctttacgctg ggcgcgattg agtttcgcca
     1861 gcggttcgcc acgccagcta atattcccct gcgcgggcga ttctaaaccc accagcaacc
     1921 gcgcgagggt acttttcccg cagccgctgc gccccagcag ggcgacggtt tcgccgcttt
     1981 tcagggtcag ggaaacgtta ttcagcaccg cctgatgttg atgttttccg ttaaatccac
     2041 cgtgcgcata gtgatgggaa aggccggaga tgttaagtaa agtcatgatg ccagctccat
     2101 accgtagagg gcgagatgag cggaaaccag gctgcgcgtc actgtatgtt tgggggcgtt
     2161 aaacagcgtt tctacatcgc cctgttcgac aatcttaccg tcagacatca ccgccacgtc
     2221 atccgccaga cgcgccacaa cgcccatatc atgggtcacc agcagcattc ccggcgcttg
     2281 tttttgcata atgctttcca gcagatcgag gatgcgcgcc tgtgctacca cgtcgaggtc
     2341 ggtggtcggt tcatcggcga tgataaacgg tgattcacac agcaccgcca tcgcaatcat
     2401 catgcgctgc aacatgccgc cgctcatctc gaacgggtac agcttcagca cgcgcgcggc
     2461 gttttccagc cccaccgctt ctatggcagc ggtaagcgta gcgtcatcgg cgggtttccc
     2521 taacgccagg caggtttccc gcgcgtgggt gtgcatggtg tgcagtggat taaaggcgct
     2581 gcgcgggttc tgcatgatgg tggcaatttt gatgccgcgc agggcgcaag gcgaaaccgg
     2641 tttgccatcg gctaaaattt cccccgccgt ctggcgaacg ccagcgggca aaatgcccag
     2701 cgtcgcggcg caggttaatg atttcccgct gccgctaccg ccgactaacg ccagcacgcg
     2761 cccgcgttgc agggttaacg atacaccgtg taccagcggc tgcgcggcct gtagcgcgat
     2821 attacgtagt tcaatctgtt gcggcattag tgtgcgtgct ccgtcaccag atgagggtcc
     2881 agatgatcgc gcagtgcgtc acccaccagg ttaaaggcca tcacgctgat aaacagcgcc
     2941 agccccggcc agaacatttg cagcggctgg gtccagatat actggcgcgc gtcgttaatc
     3001 atcacgcccc attcggcggt cggcgcggtc acaccgaggc caaggaaaga catccccgcg
     3061 acgtgcagca tcatatggcc gatatccagc gttgccagca ccagcagcga agggatcacc
     3121 gcgcctgcca gatgatcgac aaacacccgc acatggcccg caccggaaag ccgtgacgcc
     3181 agcacaaact cgcgttggcg tagtgaaatc accaggctgc gcaccatgcg tgcataccac
     3241 gcccagtgcg acagggcgat ggcgataatt acgttggtca gcccggtgcc gagcacgcca
     3301 accataaaga acgacagaat cgaggtcggg aaggtcataa acatatcggc gacgcgcatg
     3361 gtggcctgat caacgcgccc gccaatcaac ccggcgctgc cgccaataac cagccctaat
     3421 gtcagcacca gcagcaggca ggccattacc gaaccgagcg acacgcgggt cgctgtcatc
     3481 agccgcgaga aaatatcgcg acctaagtga tcggtgccca gccagtgctg cgcatccggc
     3541 gaaagcaggc gcgacggcaa atcaatcgcc tgggggtcat acggcagcca ccactggctg
     3601 gtgagcgcaa tcagcgccag cagggcgata atgatcagcg ccaggcgtac cgaccagcgg
     3661 gaagagagga aaaagttcac gcgtgcgctc cttcatgacg acgaatgcgc gggtccagcg
     3721 cggcgttgag caaatcgaca atcaaattac agaccacaaa aaccaccacc atcatcagcg
     3781 taaagcactg gatcaccgga tagtcacggt taaaaatcgc cgacaccgca tagcgcccga
     3841 cgcccggcca ggcaaagatg ttttcgataa tcatcgtccc gccaatcagt tcgccgatat
     3901 gcatccccac ggcggtgatc atcggcagcg aggcattgcg caggatgtgg cgacgttcgg
     3961 tctgtttgtc gttcaggccg cgcagacgcg cccaggtgac gtgacgctga ccggcgacgt
     4021 ccagcatact ggcgcgcagt aaacgcgcgt taatcgccag cgacataaag gcaatggaaa
     4081 ccgcaggcaa aatgatgtgc tgccagccgc cgtaacccat cgcgggtagc cattgcagat
     4141 acaccgaaaa cgccatcacc agtaaaaacg ccagccagaa gttaggcatc gacacgccaa
     4201 gaaacgcgat gaaacgtacg gcgaaatccg gcagacggtc gcgatggcgc gccgcccaga
     4261 taccgagcgg tacggaagtg agcagaatta ataccagcgc cgcacctgca agttccagcg
     4321 tggcgggcag gaagttcagc atatcgtcca gtaccgggcg ttggctggcg aatgagatac
     4381 caaagtcaag atgcagcgcc ttccacaacc aggtaccgta ctggacgtac agcggctgat
     4441 ccagcccgag catggtgcgg gtagaggcca gcatctccgg cgtcggcggc aggttagata
     4501 aacgcagata atcgagcgcc gggtcgccgg taccgaggcg cagcatcaga aaaatgatca
     4561 ccgaggcggc gagcaccatc gggatcagca gcagaaagcg gcgtaatacg taacgcaaca
     4621 ttaaggtttc accggtttaa tctgttcgaa cggaatttcg gtggcgatcg gcgcgtaggg
     4681 gatgttaccc agctccggtt ttgataccac catcattgag atgtaactga taggcagata
     4741 aaccgcctcg tcatgcagac gggtcagaat gtcgcgatac agcgcctgac gttgcgtttc
     4801 gtcatgggtc gccagcactt cgccgatctc tttatcaatc agcggtttgt cggctaatcc
     4861 ttgctgtgcc tggaagtcag cgtgtgacgg tacgcgcatt gaactgagga aggcgtgtgg
     4921 atcatatggc gcgccccagg tgcggtggaa aatcatgcca aaacgaccgt cgcgctgacg
     4981 agcatagata ctgctctctt cttcgccaat cagcgagaca tctgcgccaa tctggcgcat
     5041 atcagcctga atgatttccg ccatcgattt gcttaacgca tcggtgccga tgaacgacag
     5101 ttcaatgcgc agcggctgac catttttctc gcggatgtct ttgcccgcag gcagcgtcca
     5161 accggctttt tccagcaacg ctttcgcttt ctgcggatcg tactggcgcg gtttcaggcc
     5221 gaggttggcg tagggcacag aaggggcaaa cagggtgtcg gcgacctgct gggtgccata
     5281 caacgcgtta tcaatcagcg attttttgtt taccgcgtaa ttaagagctt cacgtactgc
     5341 cagctcgttg gtgggggctt tggcggtatt gagcgccagc atcacggttt cgatcggctg
     5401 tgacagttgg gtgtggtaag ccggattctg gctaaagcgg gcgaaggtat cgagcggtaa
     5461 taacccttcg tttccgtaca gcaggtcgat atcgccagtt tcaaacgcca ccgcgcgggt
     5521 agtcgggtcc gggatgacgt taaaggtgat ctttttaatc gctggctttt cgccccagta
     5581 gttttcgtta cggacgaaga catcgtactg attcagtttc gattcctgca aaatccacgg
     5641 tccggtgcca atcggcgctt taattccgtt catggtttca tggtttttaa actgcgaggg
     5701 agcgataaaa cggaaaggac ggggcagggc cagttcttgc aggaaaggat agtaggcgct
     5761 tttcagggta atttgcagct ctgttttact gagtgcttta acatcaacaa tctggtttgc
     5821 cagctccagc caggcgtgac gttgacggtt atcgagcact gcgcggaagt tttctgccgc
     5881 cgcctcggca tcgaacggtt caccgttgga gaatttcacg tcatcacgca gggtgaaggt
     5941 ccaggtttta ccatcttctg aatgagtcca gctttttgcc agccacggga tcaccgaacc
     6001 gtctgcctga tatttcacca atggttcata aaccatgctc tgggcgaaca tctggttagg
     6061 cgtgtaaagg tgcgggttta gtggcccgac attcaccggc caggcggtgg tgatttcatc
     6121 tggtgcagcg gcatggacga taaaagacgc acaagccagc agcgcaaata gagtgcggcg
     6181 gagtgtggag agcatggtaa ccccaatgga ttaaaataga tggcggaaat aagtatgacg
     6241 attttaagta ttcgtcatac tgattacctg ttctggatca atagatgggc agtcgggtgg
     6301 gcggatcagt taactgaatc gatccattgc acactgtcgg cggtgagcgt aaagggagta
     6361 ggggtgcaga tcgccaggct taaattttca agctgacaat ggctgactga cagcgaggag
     6421 tgataggtgc tgtctacgct gacgatttgc caggcgctgc cgccacgctg tttaacgatg
     6481 gcttctttgc gcgtccagat gcgccagaac atttccagtt gctgatcagg atgcacggcg
     6541 tccatctcag cgtgttcccc gaggctgaat acggcgttcg ccagccagtg ccagttggcg
     6601 cgcgggcgaa tcacttcgat atcgcagccg acttcgcctt catcactcaa cagcagggcg
     6661 atatcgtcac cgctatggct taagttgaac catagcggca tttccggcgc aaatgcaggt
     6721 ttgccttgtt cgccatagat gatctccggt agcggggaaa gcgtgtgcga aagcaatgca
     6781 cgccccgcca gccagcgttc gcgtcgtgga ccttgcggtg cttgctcgcg taaacccggt
     6841 ggcagtggag ctgcgcttaa ggtcgaaact ttccccagaa ctatccgata catatcaggg
     6901 ccaacgttta atggaaaatg aaagtgcgta tcgtatcact tgtcgcctca tcccggtaac
     6961 cgacttttcg gtctgcctgg ccccagtaaa atcgccagtt tgctaccgcc tttggtggtt
     7021 tccatccaga ttttacacac actggttaat ggcaccgaaa gtagcatccc taccgggccg
     7081 agcagccatc cccaaattaa caatgaaaga aataccacca tggtggacat ccccaggcga
     7141 tggcccatca tccgtggttc taaaatattg ccgatgacca tatggaccac taaaaacaat
     7201 gcgccgacca gaatacattc gtaaacacca ttaaacagca gcacctgaat cattggcggt
     7261 acggcggaaa ttaccgcgcc gatattgggc acgtagttga gcaaaaacgc cagtactgcc
     7321 cacatcagcg caaactgcac ccccatcagc tccagcccca gccagacgat gacacctgtc
     7381 cataaactga gtagcgtctt caatgcaaga tagtgcgaaa cgcctttaag tgcgcggtgt
     7441 aatcctgcga tgtgaatctg tgggttattc agcgcaaaac gcattttgta agggacgtgg
     7501 cgcacttcaa acagcataaa aactacggtc atcaccagca aaagcacgct cgccattgcc
     7561 ccggaaagcc cggtcattag cgctgtggtg aaggtaacca ctttttccga gtccatccgc
     7621 tgcagcattc gctccggcga catatgcaaa ttaagaaaag gcaacatctc ctgcaattta
     7681 aaaagtttgc gcgtcaactc cttattaaac ttcggcagca tagagataaa ttcgttaaac
     7741 gatgccgcca gtacgccgac cagcgcagtt agtgcgatca gcatcaccac caccacaatc
     7801 gtaatggcaa tggggcgttg tactccccga cgaataaacc aggtgacgag cgggttgagg
     7861 acgatggcaa aaaacagcgc cagtaacagc tgcacaatga tatctgccgc tgcgtgaatg
     7921 cccgcgagga tcactaccag cgaggccagc ttgagcagaa tgtgcatgcc cgttttatcg
     7981 ggttgagggg tttccattgg ggcttccttg tgactttttg tattaagtgt agcgggagtc
     8041 acgccagcaa tattctgatt cttactgctg attttcacgt cagcacgtgg taaaaatgaa
     8101 acactgttgt aaaaatgtgg tgatcctcat gcccgaaccc gtagccgaac ccgcgctaaa
     8161 cggattgcgc ctgaatttgc gcattgtctc cattgtcatg tttaacttcg ccagctacct
     8221 caccatcggg ttgccgctcg ctgtattacc gggctatgtc catgatgtga tgggctttag
     8281 cgctttctgg gcaggattgg ttatcagcct gcaatatttc gccaccttgc tgagccgtcc
     8341 tcatgccgga cgttacgccg atttgctggg ccccaaaaag attgtcgtct tcggtttatg
     8401 cggctgcttt ttgagcggtc tgggatatct gacggcagga ttaaccgcca gtctgcccgt
     8461 catcagcctg ttattacttt gcctgggacg cgtgatcctt gggattgggc aaagttttgc
     8521 cggaacggga tcgaccctgt ggggcgttgg cgtggttggc tcgctgcata tcgggcgagt
     8581 gatttcgtgg aacggcattg tcacttacgg ggcgatggcg atgggtgcgc cgttaggcgt
     8641 cgtgttttat cactggggcg gcttgcaggc gttagcgtta atcattatgg gcgtggcgct
     8701 ggtggccatt ttgttggcga tcccgcgtcc gacggtaaaa gccagtaaag gcaaaccgct
     8761 gccgtttcgc gcggtgcttg ggcgcgtctg gctgtacggt atggcgctgg cactggcttc
     8821 cgccggattt ggcgtcatcg ccacctttat cacgctgttt tatgacgcta aaggttggga
     8881 cggtgcggct ttcgcgctga cgctgtttag ctgtgcgttt gtcggtacgc gtttgttatt
     8941 ccctaacggc attaaccgta tcggtggctt aaacgtagcg atgatttgct ttagcgttga
     9001 gataatcggc ctgctactgg ttggcgtggc gactatgccg tggatggcga aaatcggcgt
     9061 cttactggcg ggggccgggt tttcgctggt gttcccggca ttgggtgtag tggcggtaaa
     9121 agcggttccg cagcaaaatc agggggcggc gctggcaact tacaccgtat ttatggattt
     9181 atcgcttggc gtgactggac cactggctgg gctggtgatg agctgggcgg gcgtaccggt
     9241 gatttatctg gcggcggcgg gactggtcac aatcgcgtta ttactgacgt ggcgattaaa
     9301 aaaacggcct ccggaacacg tccctgaggc cgcctcatca tcttaaaact tactgaataa
     9361 ccagcgtatt aatgatgttt tctgcggtgg tctgcgcttt ttgctgatcg tcagctggca
     9421 gagtaatttg catggtcagc agttgattac ccacgttacc cagaataacg gaagagtacg
     9481 ccgtctggcc tttcgcggag ataatactgt ctaactgctg cattttgtga cctttcagct
     9541 caatggcttt attggttacc acttgcagct gcggatcgcg gctacgttgc tgatcttcca
     9601 gacgcttcgc cagcaccgcc agatcttctt ttggatcatc gcccatgatg acgatgactg
     9661 ctttctgccc ggtggcgtcg gaccagacat gcatgttatt ggcctgcgtt cccagcttac
     9721 cgctctggtc ggtcatatcc gctggcagcg agaaacttaa cttgccatca agcaggttga
     9781 cgggattccc ggtagcgtta ctttccgcga ccgaaccctg cgccgtagcg ttagtgtctt
     9841 tatcatcaca ggccgcaagc cccataacca gcaggccaat tccgacatat ttaaccagat
     9901 tgcgcattga cttcttcctt tcgataaacg gccataacgg ctcattcatc catcttatca
     9961 caactctgat aacgaacctt taactcgcct gcaaagcgtt gatttcggat ttatctgcca
    10021 gtcttttcaa cagcatattg agtaatacgc catacattgg caggaagaaa acgatactga
    10081 ttaacacttt gaaacagtaa tcgaccagcg cgatttccat ccagtgttca gccataaagg
    10141 catccgggct acgccagaag gcaatgaaga aaaaggccag cgtgtcgctg acgttaccga
    10201 acagtgtgga cgctgtcggt gccagccacc agcggcgact ttgacgcagg cggttaaaaa
    10261 cgtgcacgtc gaggatttgc cccagcgcgt aggccatgaa actggcggtg gcgatacggg
    10321 cgacaaacag gttgaagtgg gcgagtgcgc cgaatccctg ccatgaaccc atatagaaca
    10381 gcgacgagat gacataggag attaataacg cagggatcat taccgcgaag ataatgcgtc
    10441 gggccagcgg tgcgccaaaa atacgcacgg tcaggtcggt agcaagaaaa ataaacggaa
    10501 agctaaacgc gccccaggtg gtatggaaac caaaaatgga gacgggaagc tgaaccagat
    10561 agttactgga ggtgatcacc agcagatgaa ataacgataa ccagaacaac gccttatagc
    10621 gttgagtttg cgagaaaacg ttcatattgt acctttttga ttaaccattg gggtgaggga
    10681 acccaatacg tacgacacgt ctgttacctt aacataataa cgtgcttacc cttttttcga
    10741 gccgccgcat gatactgctt tacgttgaca atgcaatggt taattttcac gcaatcgtta
    10801 acctggtttg cttacggact cacagggcgt aaactagcgc cgttttttta tgtgatgaga
    10861 agaaaatgac cgatctcttt tccagccctg accacacact cgacgcactt ggcctgcgct
    10921 gcccggaacc cgtaatgatg gtgcgcaaaa ctgtgcgcaa tatgcagccc ggcgaaacgt
    10981 tgctgattat cgccgacgat ccggccacta cccgcgatat tcctgggttt tgtaccttta
    11041 tggaacacga actggttgcg aaagagacgg atggactgcc ttatcgttat ttgattcgta
    11101 aaggcggttg atgggggctg attggcttcg atgccgcctt ttcccctcac cctaaccctc
    11161 tccccagagg ggcgagggga ccgatcgcgc tcaatgttgc gatcggtttg ccttatctcc
    11221 tgcgcaacaa tcttaacgca ttcgctgtca ccagcaccgt cgcacccgta tctgccagca
    11281 ccgccagcca tagtccggtc atccctaaca gcgtggtgac gaggaagatc cctttcagtc
    11341 ccagcgcaat ggtgatgttc tggcggatat tggcgtgagt ggcgcgtgcc agttcaatca
    11401 tttgcaccag gccgcgcagg tggttatggg ttaatgctgc atcggcggtt tccagcgcca
    11461 cgtctgtgcc gctgcccatt gcaatcccaa tggcggcagc tttcatcgct ggcgcgtcgt
    11521 taataccatc accgaccatc gccagcggcg catgttgatt cagcttggtc accgctttga
    11581 ctttctcttc cggcaatagg cccgctttaa actccagccc cagctccccg gcgattgccg
    11641 ccgctgcgcg tggattatcg ccggtgagga tcactccttt aactcccagc gcgttcagtt
    11701 cactgatggc agttgcagcg tcggcgcgca gggtatcctg caatgcaatg atacccagca
    11761 cgtcatcatt acgtactacc agcacaaccg tttgcccggc gctttccagt tcattaatca
    11821 aaccagcaaa tgcatcagcg ggatgtttcc cggcagcgca aatcaatacg cgctcaccgt
    11881 taacctgcgc ttcaatgcca gacccgacca gcgcccgctg tgattcggcg gtgggaatgg
    11941 cgagttcagc aacctgtgct tctcgcacga tggcctgcgc cagtggatgc gtcgcacctt
    12001 gctcgaccgc cgctgccagt gtcagcagtt cagattcact aatacccgtt gccggatgaa
    12061 tcgcggtaac gcgcggttta ccgacggtca gcgtaccggt tttatcaaac gccacctggg
    12121 taacacgacc tagttgttcc agcgccgctc cgcctttaat taacgcccca cgacgcgctg
    12181 ctgccgccag cccggaggta atcgccgcgg gcgtggagat aactaacgca cacgggcagc
    12241 caatcagcag cagcgtcagg cctttataaa tccactcttg ccagctggcg gcaaacagca
    12301 gcggtggcac cagcgtcacc agcagagcga cggccataat cgcgggcgta tagatacggc
    12361 tgaaacggtc gataaaccgc tcaatgggtg cgcgacgctc ttcggcttct tcgatcagtt
    12421 tcagaatccg gtcaatggcg ctggcacccg gttctgacag cacttccagc gttaccagac
    12481 ggtctacgct ggtggctcca gcagggactt tatcgcccgt tgcgcgctcc accggaatag
    12541 attcgccggt cagggcgctt tcatcaaaac tggcaaacgg tgagagcagt ttaccgtcgg
    12601 caggcaaacg tccacctgcg gcgacttcaa tcacatcgcc agggcgcagg ctgttaatcg
    12661 ccacctcttc ccgctcaccg ttacgcaggc gcgtggcggt ttctggtttc agcgccatta
    12721 acgcgctgac cccttgacgt gcgcggctgg cggcccagcc ttccagtcgt tcgccaatca
    12781 aaaacagcag caacaccatc gcagcttcag ccgttgcgcc aataaaaagc gcaccaatag
    12841 cggctacgct cattaaggtt tcaatggcga agtagctgcc ggatttgatc aaccgtaatg
    12901 cctgacgagc aatcgggtac agcccaacca gtgtggtcgc gataaacgcc agttgcccga
    12961 acggatgatt gaactgctcc agaccccagc tgattgccat catcacgatt agcgtaatca
    13021 gcggcagatt ctctttcagg cgtgatgctt gcggttcgtc ggcggactgt tcatcgcgca
    13081 gggagtagcc cgctttttgc accgcagatt caacctgggc acggatgtca ttgtcggcat
    13141 caaccaccag tttttcggtg gcgaacaaca cctgtacctg attcacgcct gcaagctggc
    13201 gcacggcatt ttctaccttg cgcgcacagg cggcgcagtc catgccgctg actttccagc
    13261 tatagcgggt gccggagacg ttttcagaga gagttggcgt gctggaacat gcgccgtcgc
    13321 agcaacagtc gttggcgttc tgtaccgtgg ttagcggttt gaacgcagca aattgagggg
    13381 ctttcttgcc gtgattgtca ggagtcgaca tggcatcctc cggttaagtt ttttctcatt
    13441 aaccgaagga tacactctgg agtcgactcc agagtcaagt tttatcagag atacagcgag
    13501 cggacgatca ggaagtgacc ggcaaagtag caggccgcag caatcgcgtt atccgcacgg
    13561 aagcgtcggc gatagtgact acccagccag acaaagttac tgataaacag caacgaagcg
    13621 ccgacaaacg cagagagcgc cggagcggtc ggacggaaga accacagttc acctgccagc
    13681 cacaccatca ccagcgtcat gccgataaac gtgcagatag gccaacgata ctcttccagc
    13741 cgcgtccaga taatcgccag taacagcgcg cccagaacca gcagcaccag tggcagaggc
    13801 cagaagaaag agagcgtcat ctgactggcg aaatagatgg tgtacagcag gtgcgagagg
    13861 aaaaacgcgc cgatggcgta catcagacgt tgacgtggca acagggttag cgcatcgccc
    13921 agcagtgagg cgcacagccc cgccagcacc agatagctaa tggcgtcgaa catcggcgct
    13981 tgccaggcca gtaacagcag gagaagaagg gttaatggtt taaataccca gcgttgccag
    14041 gttggcccac gatacgatgc atccacagat agccatgcgg aaagacagac agcgataaac
    14101 gaccaaagca tcttagttcc ttgaattcgt tattttttcg taagcggaat gcctacggtc
    14161 ttgcttccag tttagtggcg agggtggacg gatgacaaca ccataactgg cagggtatgc
    14221 ttaattccgc attttccgat gagggataaa gatgagcaaa ccacctcttt tctttattgt
    14281 tatcattggc ttaatcatcg tcgccgcatc gtttcgtttt atgcaacaac gacgggaaaa
    14341 agctgataat gatatggcac cactccagca aaagctggtg ttggtgagca acaagcggga
    14401 aaaaccgatt aacgatcgcc gttcgcgcca gcaggaagtg actccggcag gcaccagtat
    14461 gcgctatgag gcaagcttca aaccgcaaag cggaggaatg gagcagacgt ttcgcctcga
    14521 cgcccagcag taccacgccc tgacagtggg cgataaaggt acgctgagct ataaaggaac
    14581 gcgctttgtc agctttgtag gcgaacaata aggttatttt ttaactttaa atttcttctg
    14641 ccagaccagc agttcaaaca cgccgaaaag gaaaatccgt accttttcgg cggtggtcat
    14701 ctgcgggcca tctttcggta aagtggattt caatagcgcc agctgcatac catgcatcag
    14761 cacggtaaaa atcagcgcca cgttaacgaa aatattcagc ggacgtggga agggatgcac
    14821 caggttgagg attaaaaatc cccaaacaca gagcattaac aaacgaccaa tattaatcag
    14881 catcactttc tccttgtgct tcgcgttgat acagccgata agccacctga cccgccactt
    14941 tttcccgatg taatgaccag tttgccggaa cagtgggcag accgttttcg acttcgcttt
    15001 cgacataaat cagggcttcg tcagccagcc agccgttatc ttccagtaaa tttatcgtct
    15061 cttctaacaa gccacggcgg aacggtggat cgacaaacac gatattatgc ggtgtacctt
    15121 tttgcgccag gaatgacatc gcgttgctgt tcaccacgcg tgcattgcct gcttttagtg
    15181 tcgccagatt cttaattaac tgctgagaaa ccgcgcgatc catctcaatc aacgttgccc
    15241 ccgcagcata gcgcgacaac gcttccagcc ccagcgcgcc gcttccggcg aagcaatcca
    15301 gacattgggc gtcaacaatg accggagcca gccagttaaa caacgtttcg cgtacgcggt
    15361 cggtggtggg gcgcagacct gggctatcag gaaccgggag tttacggcct cgccactgcc
    15421 cgccaataat gcggatttgg ccgctgccgg aatgattcgg ttttttcatg ataaattgct
    15481 caatccgcca gataacacat acttgcaggc ggtgatgtga attaagttaa gtgatagact
    15541 atttcatcat ttttttagct gctatgtaca tagcgttaac gctgtgccat gaagcaacag
    15601 cgaggagtgt agtcgcaaat ggcgaaagaa aaaaaacgtg gctttttttc ctggctgggc
    15661 tttggtcaaa aagagcagac cccggaaaaa gagacagaag ttcagaatga acaaccggtt
    15721 gtagaagaaa tcgttcaggc gcaagagcct gtgaaggctt cggaacaagc cgttgaagag
    15781 cagccgcagg cgcatactga agccgaggcg gaaacttttg ctgccgacgt tgtggaagtc
    15841 actgaacagg ttgctgaaag tgaaaaagcg cagcctgaag cggaagtcgt tgcacagccg
    15901 gaaccggtcg cagaagaaac gccggagcca gtggctatcg aacgtgaaga gctgccgttg
    15961 ccagaagacg tcaacgccga agaggtttcg ccagaagagt ggcaggctga agcggaaacc
    16021 gtagagattg tcgaagcggc agaagaagaa gcggctaaag aagaaatcac cgacgaagag
    16081 ctggaagcac aggcgctggc tgccgaagcg gcagaagagg cggtgatggt agttcctccg
    16141 gcagaagaag agcagccggt ggaagaaatt gcccaggagc aagaaaaacc gaccaaagaa
    16201 ggtttcttcg cgcgcctgaa acgcagcctg ttaaaaacca aagaaaatct cggttccgga
    16261 tttatcagcc tgttccgcgg taaaaaaatc gacgatgatc tgtttgagga gctggaagaa
    16321 cagctgttga tcgccgatgt gggcgtggaa accacacgta aaattatcac caatctgacg
    16381 gaaggcgcat cccgcaagca gcttcgtgat gccgaggcgc tctatggcct gctgaaagaa
    16441 gagatgggcg agattctggc gaaagtcgat gagccgctga atgttgaagg taaaacgccg
    16501 ttcgtgatcc tgatggtggg cgtcaacggt gtgggtaaaa ctacgacgat tggtaagctg
    16561 gcacgtcagt ttgagcagca gggtaaatcg gtgatgctgg cggcgggcga tactttccgt
    16621 gcagcagcgg ttgaacagct tcaggtctgg ggtcagcgca acaatattcc ggtgattgcc
    16681 cagcataccg gtgcggattc cgcctctgtt atcttcgacg ccattcaggc agctaaagcg
    16741 cgtaatatcg acgtcctgat tgccgatacc gccggacgcc tgcagaacaa atcgcacctg
    16801 atggaagagt tgaagaaaat cgtccgcgtg atgaagaaac tcgacgttga agcgccgcat
    16861 gaagttatgc tgactattga tgccagtacc gggcagaatg cggtaagcca ggccaaactg
    16921 ttccacgaag ccgttggctt aaccggcatc acgctaacga aactggacgg cacggcgaaa
    16981 ggcggggtaa ttttctcggt ggctgaccag tttggtatcc ctatccgcta cattggtgtc
    17041 ggcgaacgta ttgaggattt gcgtccgttt aaggcggacg actttataga ggcacttttt
    17101 gcccgagagg attaacaatg attcgctttg aacatgtcag caaggcttat ctcggtggga
    17161 gacaggcgct gcagggcgtt acgttccata tgcagccggg tgagatggcg tttctgaccg
    17221 gtcattccgg cgcagggaaa agtaccctcc tgaagctgat ctgtgggatt gagcggccca
    17281 gcgccgggaa aatctggttt agcggccatg acatcacgcg tctgaaaaac cgtgaagttc
    17341 cgtttctgcg ccgccagatt ggcatgattt tccaggatca ccatctactg atggaccgta
    17401 ctgtctacga taacgtggcg atcccgctga ttatcgctgg tgccagcggt gacgatattc
    17461 gtcgccgggt gtcggcggca ctggataaag tcgggctact ggacaaagcg aagaacttcc
    17521 ctattcagct ttccggcggt gaacaacagc gtgttggcat tgcccgcgcg gtggtgaata
    17581 agcccgcggt actgctggcg gacgaaccga ccggtaacct ggacgacgcg ttgtcggaag
    17641 ggattctacg tctgtttgaa gagtttaacc gcgttggggt aaccgtattg atggcaacgc
    17701 acgacatcaa cctgatttca cggcgttcct atcgcatgct caccctgagc gatggtcact
    17761 tgcatggagg cgtgggccat gaataagcgc gatgcaatca atcatattcg gcagtttggc
    17821 gggcgtcttg atcgcttccg taaatcggtc ggcggctctg gcgacggcgg tcgtaacgcc
    17881 ccgaaacgcg cgaaatcctc gccaaaaccg gtaaatcgta aaaccaacgt tttcaacgaa
    17941 caggtgcgct atgccttcca cggcgcattg caggatctga aaagcaaacc gttcgccacg
    18001 tttttaacgg tgatggttat cgccatctcc ctgacgctgc ccagcgtctg ttatatggtg
    18061 tacaaaaacg ttaatcaggc ggcgacgcag tattatccgt caccgcaaat cactgtttac
    18121 ttgcaaaaaa cgctggacga tgacgccgct gcgggcgtgg tggcacagtt gcaggccgag
    18181 caaggcgtgg agaaagtgaa ctatctttct cgtgaagacg cactgggcga gttccgtaac
    18241 tggtctggtt ttggtggtgc gctggatatg ctggaagaaa acccgcttcc ggcagtggcg
    18301 gtggtgatcc cgaaactcga ttttcagggg acggaatcac tcaatacgct gcgtgatcgt
    18361 atcacgcaga ttaacggcat tgacgaagtg cggatggatg acagctggtt tgcccgtctg
    18421 gcggcgttga ccgggctggt cgggcgcgtt tcggcgatga tcggcgtgtt gatggtggcg
    18481 gcagtgttcc tcgtcatcgg caacagcgta cgtctgagca tctttgctcg ccgtgactcc
    18541 atcaacgtgc agaaactgat tggtgcgacg gatggtttca tccttcgccc gttcctgtat
    18601 ggtggcgcac tgctgggatt ttctggcgca ttgttgtcat taattttgtc agaaattctg
    18661 atgctgcgat tgtcatcggc ggttgcggaa gtggcacagg ttttcggaac gaagtttgat
    18721 atcaatggtt tatcgttcga tgaatgcctg ctattgctgc tggtatgctc gatgattggc
    18781 tgggtggcag cgtggcttgc cacggtacaa catttacgcc actttacgcc tgaataataa
    18841 aagcgtgtta tactctttcc ctgcaatggg ttccgtagca gggaaagaga tcccgttgtc
    18901 tcttcccggt atttcatctc tatgtcacat tttgtgcgta atttattcac aagcttgcat
    18961 tgaacttgtg gataaaatca cggtctgata aaacagtgaa tgataacctc gttgctctta
    19021 agctctggca cagttgttgc taccactgaa gcgccagaag atatcgattg agaggatttg
    19081 aatgactgac aaaatgcaaa gtttagcttt agccccagtt ggcaacctgg attcctacat
    19141 ccgggcagct aacgcgtggc cgatgttgtc ggctgacgag gagcgggcgc tggctgaaaa
    19201 gctgcattac catggcgatc tggaagcagc taaaacgctg atcctgtctc acctgcggtt
    19261 tgttgttcat attgctcgta attatgcggg ctatggcctg ccacaggcgg atttgattca
    19321 ggaaggtaac atcggcctga tgaaagcagt gcgccgtttc aacccggaag tgggtgtgcg
    19381 cctggtctcc ttcgccgttc actggatcaa agcagagatc cacgaatacg ttctgcgtaa
    19441 ctggcgtatc gtcaaagttg cgaccaccaa agcgcagcgc aaactgttct tcaacctgcg
    19501 taaaaccaag cagcgtctgg gctggtttaa ccaggatgaa gtcgaaatgg tggcccgtga
    19561 actgggcgta accagcaaag acgttcgtga gatggaatca cgtatggcgg cacaggacat
    19621 gacctttgac ctgtcttccg acgacgattc cgacagccag ccgatggcac cggtgctcta
    19681 tctgcaggat aaatcatcta actttgccga cggcatcgaa gatgataact gggaagagca
    19741 ggcggcaaac cgtctgaccg acgcgatgca gggtctggac gaacgcagcc aggacatcat
    19801 ccgtgcgcgc tggctggacg aagacaacaa gtccacgttg caggaactgg ctgaccgtta
    19861 cggtgtttcc gctgaacgtg tgcgccaact ggaaaagaac gcgatgaaaa aacttcgcgc
    19921 cgctattgaa gcgtaatttc cgctattaag cagagaaccc tggatgagag tccggggttt
    19981 ttgttttttg ggcctctaca ataatcaatt ccccctccgg caaaacgtca atccccacgc
    20041 agattgttaa taaactgtca aaatatctat tccaatatca taaaaatggg gtatgtttta
    20101 gcagagtatg ctgctaaagc acgggtagtc atgcataaaa cgaaataaag tgctgaatca
    20161 caacaccaca acacacgtaa taaccagaag aatggggatt ctcaggatga atataaaggg
    20221 taaagcgtta ctggcaggat gtatcgcgct ggcattcagc aatatggctc tggcagaaga
    20281 tattaaagtc gcggtcgtgg gcgcaatgtc cggtccggtt gcgcagtacg gtgaccagga
    20341 gtttaccggc gcagagcagg cggttgcgga tatcaacgct aaaggcggca ttaaaggcaa
    20401 caaactgcaa atcgtaaaat atgacgatgc ctgtgacccg aaacaggcgg ttgcggtggc
    20461 gaacaaagtc gttaacgacg gcattaaata tgtgattggt cacctctgtt cttcatcaac
    20521 gcagcctgcg tctgatatct acgaagacga aggcatttta atgatcaccc cagcggcaac
    20581 cgcgccggag ctgaccgccc gtggctatca gctgatcctg cgcaccaccg gcctggactc
    20641 cgaccagggg ccgacggcgg cgaaatatat tcttgagaaa gtgaaaccgc agcgtattgc
    20701 tatcgttcac gacaaacagc aatacggcga aggtctggca cgagcggtgc aggacggcct
    20761 gaagaaaggc aatgctaacg tggtgttctt tgatggtatc accgccgggg aaaaagattt
    20821 ctctacgctg gtggcgcgtc tgaagaaaga gaatatcgac ttcgtttact acggcggtta
    20881 ccacccggaa atggggcaaa tcctgcgtca ggcacgcgcg gcagggctga aaactcagtt
    20941 tatggggccg gaaggtgtgg ctaacgtttc gctgtctaac attgcgggcg aatcagcgga
    21001 agggctgctg gtgaccaagc cgaagaacta cgatcaggtt ccggcaaaca aacccattgt
    21061 tgacgcgatc aaagcgaaaa aacaggaccc aagcggcgca ttcgtttgga ccacctacgc
    21121 cgcgctgcaa tctttgcagg cgggcctgaa tcagtctgac gatccggctg agatcgccaa
    21181 atacctgaaa gcgaactccg tggataccgt aatgggaccg ctgacgtggg atgagaaagg
    21241 cgatctgaaa ggctttgagt tcggcgtatt tgactggcac gccaacggca cggcgaccga
    21301 tgcgaagtaa tcattaatcg gcaactttgg gttgccgcca aattgctaat atcgagtacg
    21361 ttgcttcatg ccggatgcgg cgtaaacgcc ttatccggcc tacaagatcc aaagaaatca
    21421 gtaaattgca acacacattg taggcctgat aagcgtagcg catcaggcaa tttctcgttg
    21481 gtcatcactt ttgaaatcaa cttgacgatt aacgcttctc ccagccgccc tgttgcgccg
    21541 taaaccccag cgcctgcata aacgccgtca tcacaccgcg atcttccaag cctgcatccg
    21601 ccatccacca gcatgaaacg ccaggattgt tacgcaaaac ctcttccagc agatattgcc
    21661 ccacaccgcg acgtcgggtg acttcccgca cacgcaggga atccagtgct ccctcggtgc
    21721 cgcttaaggt tacccgtacg gcagcgagca ggcgctcgtt aaaacgcgcg gcgtagatac
    21781 ggtggttatc gtcaacctgt aaagaggaag gggaatactc cggccagatc ttttgcaggt
    21841 caatccggtc ctggtcgcta aagttttcta atcgaatgat ggtcagcttc atgggtaacc
    21901 cgtgtaaatc acaaaagtgg aaccagtgta gcgaaataat ttaatcggag gctttctctt
    21961 ttttatttct tttggcaggt gattaatttt ttaa
//
LOCUS       DAZFWL020000074        21750 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFWL020000074 DAZFWL020000000
VERSION     DAZFWL020000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.074"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(19..1665)
                     /gene="fumB"
                     /locus_tag="ACHKNP_RS14785"
                     /old_locus_tag="pgaptmp_002957"
     CDS             complement(19..1665)
                     /gene="fumB"
                     /locus_tag="ACHKNP_RS14785"
                     /old_locus_tag="pgaptmp_002957"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU4705086.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            1864..3039
                     /gene="manA"
                     /locus_tag="ACHKNP_RS14790"
                     /old_locus_tag="pgaptmp_002958"
     CDS             1864..3039
                     /gene="manA"
                     /locus_tag="ACHKNP_RS14790"
                     /old_locus_tag="pgaptmp_002958"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HGU4705087.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSILQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            3140..4648
                     /gene="ydgA"
                     /locus_tag="ACHKNP_RS14795"
                     /old_locus_tag="pgaptmp_002959"
     CDS             3140..4648
                     /gene="ydgA"
                     /locus_tag="ACHKNP_RS14795"
                     /old_locus_tag="pgaptmp_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU4705088.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(4693..5958)
                     /gene="uidC"
                     /locus_tag="ACHKNP_RS14800"
                     /old_locus_tag="pgaptmp_002960"
     CDS             complement(4693..5958)
                     /gene="uidC"
                     /locus_tag="ACHKNP_RS14800"
                     /old_locus_tag="pgaptmp_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HGU4705089.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5997..7370)
                     /gene="uidB"
                     /locus_tag="ACHKNP_RS14805"
                     /old_locus_tag="pgaptmp_002961"
     CDS             complement(5997..7370)
                     /gene="uidB"
                     /locus_tag="ACHKNP_RS14805"
                     /old_locus_tag="pgaptmp_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HGU4705090.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(7367..9178)
                     /gene="uidA"
                     /locus_tag="ACHKNP_RS14810"
                     /old_locus_tag="pgaptmp_002962"
     CDS             complement(7367..9178)
                     /gene="uidA"
                     /locus_tag="ACHKNP_RS14810"
                     /old_locus_tag="pgaptmp_002962"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HGU4705091.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     AEQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(9567..10157)
                     /gene="uidR"
                     /locus_tag="ACHKNP_RS14815"
                     /old_locus_tag="pgaptmp_002963"
     CDS             complement(9567..10157)
                     /gene="uidR"
                     /locus_tag="ACHKNP_RS14815"
                     /old_locus_tag="pgaptmp_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HGU4705092.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAERMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(10378..11145)
                     /gene="hdhA"
                     /locus_tag="ACHKNP_RS14820"
                     /old_locus_tag="pgaptmp_002964"
     CDS             complement(10378..11145)
                     /gene="hdhA"
                     /locus_tag="ACHKNP_RS14820"
                     /old_locus_tag="pgaptmp_002964"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HGU4705093.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(11257..12285)
                     /gene="malI"
                     /locus_tag="ACHKNP_RS14825"
                     /old_locus_tag="pgaptmp_002965"
     CDS             complement(11257..12285)
                     /gene="malI"
                     /locus_tag="ACHKNP_RS14825"
                     /old_locus_tag="pgaptmp_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HGU4705094.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            12460..14052
                     /gene="malX"
                     /locus_tag="ACHKNP_RS14830"
                     /old_locus_tag="pgaptmp_002966"
     CDS             12460..14052
                     /gene="malX"
                     /locus_tag="ACHKNP_RS14830"
                     /old_locus_tag="pgaptmp_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HGU4705095.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVLALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            14062..15234
                     /gene="malY"
                     /locus_tag="ACHKNP_RS14835"
                     /old_locus_tag="pgaptmp_002967"
     CDS             14062..15234
                     /gene="malY"
                     /locus_tag="ACHKNP_RS14835"
                     /old_locus_tag="pgaptmp_002967"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HGU4705096.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRLLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            15338..16339
                     /gene="add"
                     /locus_tag="ACHKNP_RS14840"
                     /old_locus_tag="pgaptmp_002968"
     CDS             15338..16339
                     /gene="add"
                     /locus_tag="ACHKNP_RS14840"
                     /old_locus_tag="pgaptmp_002968"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HGU4705097.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16374..17414)
                     /gene="ydgJ"
                     /locus_tag="ACHKNP_RS14845"
                     /old_locus_tag="pgaptmp_002969"
     CDS             complement(16374..17414)
                     /gene="ydgJ"
                     /locus_tag="ACHKNP_RS14845"
                     /old_locus_tag="pgaptmp_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU4705098.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            17657..17782
                     /gene="blr"
                     /locus_tag="ACHKNP_RS14850"
                     /old_locus_tag="pgaptmp_002970"
     CDS             17657..17782
                     /gene="blr"
                     /locus_tag="ACHKNP_RS14850"
                     /old_locus_tag="pgaptmp_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HGU4705099.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            18055..18270
                     /gene="ydgT"
                     /locus_tag="ACHKNP_RS14855"
                     /old_locus_tag="pgaptmp_002971"
     CDS             18055..18270
                     /gene="ydgT"
                     /locus_tag="ACHKNP_RS14855"
                     /old_locus_tag="pgaptmp_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HGU4705100.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            18356..18796
                     /gene="ydgK"
                     /locus_tag="ACHKNP_RS14860"
                     /old_locus_tag="pgaptmp_002972"
     CDS             18356..18796
                     /gene="ydgK"
                     /locus_tag="ACHKNP_RS14860"
                     /old_locus_tag="pgaptmp_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HGU4705101.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            18873..19454
                     /gene="rsxA"
                     /locus_tag="ACHKNP_RS14865"
                     /old_locus_tag="pgaptmp_002973"
     CDS             18873..19454
                     /gene="rsxA"
                     /locus_tag="ACHKNP_RS14865"
                     /old_locus_tag="pgaptmp_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HGU4705102.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAIGFSLVMVLFAAIRERLAV
                     ADVPAPFRGHAIALITAGLMSLAFMGFSGLVKL"
     gene            19454..20032
                     /gene="rsxB"
                     /locus_tag="ACHKNP_RS14870"
                     /old_locus_tag="pgaptmp_002974"
     CDS             19454..20032
                     /gene="rsxB"
                     /locus_tag="ACHKNP_RS14870"
                     /old_locus_tag="pgaptmp_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HGU4705103.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            20025..>21750
                     /gene="rsxC"
                     /locus_tag="ACHKNP_RS14875"
                     /old_locus_tag="pgaptmp_002975"
     CDS             20025..>21750
                     /gene="rsxC"
                     /locus_tag="ACHKNP_RS14875"
                     /old_locus_tag="pgaptmp_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HGU4705104.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQ"
BASE COUNT         5314 a         5619 c         5323 g         5494 t
ORIGIN      
        1 cccgaagggc ggctctgttt atttcacaca gcgagtgcac tgtgtgagtt gtatctgctg
       61 gaagaagtca tttcctttat catccacaag gataaacgcc gggaaatctt ccacttcaat
      121 tttccagatg gcttccattc ccagttccgg atattcaaca cattccaggc tcttaatact
      181 tccctgcgcc aatacagcgg ccggaccacc gatactgcca aggtagaagc cgccgtgttt
      241 tttacaggca tccgtcacct gctggctgcg gttgcctttc gccagcatga tcatacttcc
      301 gccctgcgct tgcagttgat cgacataaga atccatccgt ccggcggtcg ttgggccaag
      361 agaaccggag gcataacctt ccggcgtttt ggccggaccc gcgtagtaaa tcggatgatc
      421 tttaatgtac tgcggcagcc cttcaccgtt atccatccgc tctttcagtt tggcgtgagc
      481 aatatcacga ccgacgataa tcgtgccgtt aagcgataag cgtgtagaaa cgggatactg
      541 cgacaactgt gcgaggatct ctttcatcgg acggttaagg tcaacgcgca ccgcttcgcc
      601 ttctcccgct ttacgcagct cttcagggat atatttacct ggattatgtt ccagtttttc
      661 gatccagatc ccctgacggt tgatcttcgc tttgatatta cggtcagcag agcaggaaac
      721 acccatacca accggacagg atgcgccgtg acgtggcagg cgaatcacgc gtatgtcgtg
      781 agcgaagtat ttaccaccaa actgcgcacc cagaccgaga ttttgcgctt cgatcagcaa
      841 ttctttttcc agttccacat cgcggaatgc ctgaccgtgc tcattccctt ccgttggcag
      901 ttcgtcatag tatttagcgg aagccagttt cactgtttta aggttagttt ctgcagaagt
      961 tccaccaatt acgaacgcaa tatgatacgg aggacaggcc gccgtaccca gcgtgcgcat
     1021 cttctcaacc aggtaatttt tcagtttccc cggcgttagt aacgctttgg tttcctgata
     1081 gagatacgtc ttgtttgccg aaccaccacc tttggcgata cagaggaatt tgtactcgtc
     1141 gccatcaacg gcataaagat cgatctgcgc tggcagattg gtgccggtat tcacctcttt
     1201 atacatatcc agcggcgcgt tttgcgagta gcgcaaatta tcttcgatat aagtgttata
     1261 gacaccgcgc gccagcgccg cttcatcaca gccaccggtc catacacgct gccctttttt
     1321 accaacaata atcgcagtgc cggtatcctg acaggttggc agaacgcctt tcgccgcgat
     1381 gtcggagtta cgcaggaatt gcagcgccac atatttatca ttttcgctgg cctccgggtc
     1441 acgcagaatg tcggccacct gttgttggtg agccggacgc agcataaacg acgcatcatg
     1501 aaacgcctga cgcgccaaca gagttaacgc ttcgggtgcg actttcaaaa tctcctgccc
     1561 ttcaaattca gatacgctaa cgtgttcgct ggttagcagg taatactcag tatcatcttt
     1621 tttgagtgga aaaggagcct gataatgaaa gggtttgttt gacattgttc tctcacttac
     1681 tgcctggttt ggttatgctc tgggcgggtg ttccgttgcc ctgttaaaag cgagtaacaa
     1741 tatcctacac acttttttaa caaaaactga gactagtacg actttttgcg gctccaggtt
     1801 acttcccgta ggattcttgc tttaatagtg ggattaattt ccacattaaa acagggattg
     1861 atcatgcaaa aactcattaa ctcagtgcaa aactatgcct ggggcagcaa aacggcgttg
     1921 actgaacttt atggtatgga aaatccgtcc agccagccga tggccgagct gtggatgggc
     1981 gcacatccga aaagcagttc acgagtgcag aatgccgccg gagatatcgt ttcactgcgt
     2041 gatgtgattg agagtgataa atcgactctg ctcggagagg ccgttgccaa acgctttggc
     2101 gaactgcctt tcctgttcaa agtattatgc gcagcacagc cgctctccat tcaggttcat
     2161 ccaaacaaac gcaattctga aatcggtttt gccaaagaaa atgccgcagg tatcccgatg
     2221 gatgccgccg agcgtaacta taaagatcct aaccacaagc cggagctggt ttttgcgctg
     2281 acgcctttcc ttgcgatgaa cgcgtttcgt gagttttccg agattgtctc catactccag
     2341 ccagtcgcag gtgcacatcc ggcaattgct cactttttac aacagcctaa tgccgaacgt
     2401 ttaagcgaac tgttcgccag cctgttgaat atgcagggtg aagaaaaatc ccgcgcgctg
     2461 gcgattttaa aatcggcgct cgacagtcag cagggtgaac cgtggcaaac gattcgttta
     2521 atttctgaat tttacccgga agacagcggc ctgttctccc cgctgttact gaatgtggtg
     2581 aaattgaatc ctggcgaagc gatgttcctg ttcgctgaaa caccgcacgc ttacctgcaa
     2641 ggcgtggcgc tggaagtgat ggcgaactcc gataacgtgc tgcgtgcggg tctgacgcct
     2701 aagtacattg atattccgga actggttgcc aatgtgaaat tcgaagccaa accggctaac
     2761 cagttgttga cccagccggt gaaacaaggt gcagaactgg acttcccgat tccagtggat
     2821 gattttgcct tctcgctgca tgaccttagt gataaagaaa ccaccattag ccagcagagt
     2881 gccgccattt tgttctgcgt cgaaggcgat gcaacgttgt ggaaaggttc tcagcagtta
     2941 cagcttaaac cgggtgaatc agcgtttatt gccgccaacg aatcaccggt gactgtcaaa
     3001 ggccacggcc gtttagcgcg tgtttacaac aagctgtaag agcttactga aaaaattaac
     3061 atctcttgct aagcttatta aaggcttata acaccttcag gcggccagtc cgcctgattt
     3121 cattttatgg ataatcatta tgaataaatc gctggtagcg gtaggcgtca ttgttgcgct
     3181 aggcgtagtc tggacaggcg gcgcatggta tacaggcaag aagattgaaa cccatctcga
     3241 agacatggtc gcgcaggcga acgcgcaact caaactgaca gctcctgaat ccaacctgga
     3301 agtgagttat caaaactatc atcgcggcgt attcagcagc cagttgcaac tgttggtgaa
     3361 acccattgcc gggaaagaaa atccgtggat taaaagcggt cagagcgtca tcttcaacga
     3421 atcggttgat catggtccct tcccgcttgc ccagcttaaa aaactgaacc tgatcccgtc
     3481 gatggcatca attcaaacca cgctggttaa taacgaagta agcaaaccac tgtttgatat
     3541 ggcaaaaggt gaaacgcctt ttgagattaa ctcgcgcatt ggttacagcg gtgattccag
     3601 ttccgatatt tcgctcaagc cactgaatta cgagcaaaag gatgaaaaag tcgcctttag
     3661 cggcggcgag ttccagttaa atgctgacag agacggcaaa gccatctccc tttccgggga
     3721 ggcgcaaagt ggtcggatag acgcagttaa cgaatacaac cagaaagtgc agttgacctt
     3781 taataatctg aaaaccgacg gttccagcac gctggcaagt tttggtgagc gcgtaggaaa
     3841 ccaaaaactg tcactggaaa aaatgaccat ttcagtggaa ggcaaagaac tggcactgct
     3901 ggaaggcatg gagatcagcg gtaaatcgga tctggtcaat gacggtaaaa cgatcaatag
     3961 ccaactggat tactcgctaa acagcctgaa ggtacagaat caggatctgg gcagcggcaa
     4021 gctgacttta aaagtcggcc agattgatgg tgaagcctgg catcagttta gccagcaata
     4081 taacgcgcaa actcaggcgc tgctggcaca gccagaaatt gccaacaatc ccgaacttta
     4141 tcaggagaaa gtgacggaag ccttctttag cgccctgccg ctgatgttga aaggcgatcc
     4201 ggtgattact atcgcgccgc taagctggaa aaacagtcag ggtgaaagtg cgctgaatct
     4261 gtcgctgttc ctgaaagatc cggcaacgac taaagaagcg ccgcaaacgc tggcgcagga
     4321 agtagatcgt tcggttaaat ctctggatgc gaaactgacc attccggtgg atatggcaac
     4381 tgagtttatg actcaggtag cgaagctgga aggttatcag gaagatcaag cgaaaaaact
     4441 ggcgaaacag caagttgaag gtgcatcagc aatggggcag atgttccgtc tgaccacctt
     4501 gcaggacaat accatcacca ccagcctgca atatactaac ggtcagataa cgttaaacgg
     4561 gcagaaaatg ccactggaag atttcgttgg tatgtttgca atgccggcat taaatgttcc
     4621 ggtcgtaccc gctattccgc agcagtaatt cacagacaaa acgccggaga tttttctctc
     4681 cggcgttatt ttttacttca gcataaagtc atactgaata ctcattttca catcccagta
     4741 attcaggcga tcgcccagcg tactgccatc gcgcttttcc cgccctttat cggcagcaag
     4801 aaagaaattc atcccttgta acggcccttc gctgacccga tagttcatac cacctaagat
     4861 acccgtgcct tcaatccgca catctcgttt actggattca tagccgcgag tcacaatcgg
     4921 tgcaaatgtc agcgtaaatt gcggcgttaa acgatagttg acgcccaatt gccaggactg
     4981 cattccttca cggttacgat caagacttaa tgaaaaggga tagccaatgt cggtatccac
     5041 cacggcacca ttcattgcat ggcgcaaata accaccactg ccaaatacgc ttccccacgg
     5101 cgcattccag gtcaattgtc cactaaccaa cgccgtttca ttgggctggc tggtattacg
     5161 acttaaacct tcgagctgcg cgtaaaagcc gagcaactcc cctttcaacg tataattcgg
     5221 gtcgagcggt atgcttttct cgagaatact ttgtatctga ttggaaacat ctttctgtac
     5281 cgatgccccc agagaatagc gactggtatc atcatgccaa ctggcggcta aaaaagaacg
     5341 tgcgcgatgt ttatcgtatt ttcccgtctg gctgttatac accccttcat cacgaatacc
     5401 ggttgttgaa tcagtccata aaaatactcg tgaacgccac attccccaca gatcaatatt
     5461 tggtgttaaa gcccaataac ctaatgctcc ttcagaaact gtcggtaatg tacgttgcga
     5521 actgccagcc attaacggaa tacgataagg taagctacca taactataat ccgtcccgaa
     5581 gcgacctagt ttaagcaggc tattttcact gggttttatt tttactgccc ccagggcaaa
     5641 gccaaaactt ttatcaccat caagatacca ccgggtactc atatcagcac gagcacctaa
     5701 tttataaaca taatatgccc cgccttcaac gccaataata ttggaataat aaccactatt
     5761 gaaatcgagc aatcctccct gtacccatgc gtaaatatct cttcccgcgc cagcactggg
     5821 cttatccgcc ctgcgcaatt cgtttttcag tctgatgcgt agtcccgctt catcgtcagc
     5881 cagttgcgcc gcataagcag aggtaaggcc agaggcagcc gtcaggcaaa taacggccat
     5941 ggccactatt tttctcatag ttgcaccttt gatgttctga ttatttttat tgaatattaa
     6001 ttagtgatat cgctgattaa ttgctgctgc acttttttac gattatcaat ttcaaccacg
     6061 atttctttga attttttatc cgtgagcgga taaaaccaga taataacgaa tgccagtagc
     6121 ataaatccgc aaggtactaa ggcaattgat gtgcggatgc ccataataac ttccggcgtt
     6181 tgcacctgat tggcgatata tccgcttaat cccaaaataa aggcaggaat tgaacctccg
     6241 attgcctgac cgcatttacg ggtaaatgag aatagtgaat aggtgagccc ttcaattcgc
     6301 acgccggtca ggtattcacc gtattctacg gtatcagctt ccagtgccca catcacagtc
     6361 atagtaacgc cctgaccaat tgaagcgatg gccaacgcaa ccaacgccac cggcagtgac
     6421 cagacggaaa cccagaagaa tagcagataa ccgcaggttc ccagcaaagc gccaatcagg
     6481 aaggtattct ttttaccgat cctcgcgacc atccccggca ccagcggtgc cgatgccaca
     6541 gtaccaacca ggttttgcac cagtaccagc acagtgaaca gcccggtatc atttaacaca
     6601 tagcgcacgt agaacaacga cgaggcgctg accgcgaagg tcgaaatcag cacacacagc
     6661 gcaccgatgc acaacataaa cagcgggcga ttccgtttca gggtttgcag actgatcttc
     6721 aatgacggct gcgccacgat acgtaccaca ttctcacgcg tcgatttgaa gcagatgaag
     6781 taaagcacca ttccggcaat cgccagcaca atcgtccaga aatggtatac cgacaccatc
     6841 tcttccgggc tggagttctt aatgctcggt cctatcagaa atgccaggca gacaaaggtt
     6901 aatgaagcgg caatcccgcg agccgcgccc agacgggcgc gggattgtgg ttgttgggtc
     6961 atcgcggtag caagtgaacc ataaggaata ttcaccaggc tgtagcaaag cccgaggccc
     7021 atgtaggtca aatatgcata caccactttg ctaccatggc tccagtcggt cggcacccag
     7081 aataccagca cgctgaagat cattaacggc gcagtaccga agagtaaaaa cgggcggaat
     7141 tttccccagc gggtattcac actgtccacc actcgtccgg caaagacgtc ggcgaaggca
     7201 tcgaataccc gcaccagtaa cagcatggtg cccgccgcag cggcaccgac gccagcgacg
     7261 tcggtgtagt aactcaatag gaagagcgcc cccattgcga aggcgaagtt attggcgacg
     7321 tcaccgaggc tgtagccgac gatggtgcgc caggagagtt gttgattcat tgtttgcctc
     7381 cctgctgcgg tttttcaccg aaattcatgc cagtccagcg tttttgcagc agaaaagccg
     7441 ccgacttcgg tttgcggtcg cgagtgaaga tccctttctt gttaccgcca acgcgcaata
     7501 tgccttgcga ggtcgcaaaa tcggcgaaat tccatacctg ttcaccgacg acggcgctga
     7561 cgcgatcaaa gacgcggtga tacatatcca gccatgcaca ctgatactct tcactccaca
     7621 tgtcggtgta cattgagtgc agcccggcta acgtatccac gccgtattcg gtgatgataa
     7681 tcggctgatg cagtttctcc tgccaggcca gaagttcttt ttccagtacc ttctctgccg
     7741 tttccaaatc gccgctttgg acataccatc cgtaataacg gttcaggcac agcacatcaa
     7801 agagatcgct gatggtatcg gtgtgagcgt cgcagaacat tacattgacg caggtgatcg
     7861 gacgcgtcgg gtcgagttta cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac
     7921 cttgcggacg ggtatccggt tcgttggcaa tactccacat caccacgctt gggtggtttt
     7981 tgtcacgcgc tatcagctct ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga
     8041 ctgcctcttc gctgtacagt tctttcggct tgttgcccgc ttcgaaacca atgcctaaag
     8101 agaggttaaa gccgacagca gcagtttcat caatcaccac gatgccatgt tcatctgccc
     8161 agtcgagcat ctcttcagcg taagggtaat gcgaggtacg gtaggagttg gccccaatcc
     8221 agtccattaa tgcgtggtcg tgcaccatca gcacgttatc gaatcctttg ccacgcaagt
     8281 ccgcatcttc atgacgacca aagccagtaa agtagaacgg tttgtggtta atcaggaact
     8341 gttcgccctt cactgccact gaccggatgc cgacgcgaag cgggtagata tcacactctg
     8401 tctggctttt ggctgtgacg cacagttcat agagataacc ttcacccggt tgccagaggt
     8461 gcggattcac cacttgcaaa gtcccgctag tgccttgtcc agttgcaacc acctgttgct
     8521 ccgcatcacg cagttcaacg ctgacatcac cattggccac cacctgccag tcaacagacg
     8581 cgtggttaca gtcttgcgcg acatgcgtca ccacggtgat atcgtccacc caggtgttcg
     8641 gcgtggtgta gagcattacg ctgcgatgga tcccggcata gttaaagaaa tcatggaagt
     8701 aagactgctt tttcttgccg ttttcgtcgg taatcaccat tcccggcggg atagtctgcc
     8761 agttcagttc gttgttcaca caaacggtga tacgtacact tttcccggca ataacatacg
     8821 gcgtgacatc ggcttcaaat ggcgtatagc cgccctgatg ctccatcact tcctgattat
     8881 tgacccacac tttgccgtaa tgagtgaccg catcgaaacg cagcacgata cgctggcctg
     8941 cccaaccttt cggtataaag acttcgcgct gataccagac gttgcccgca taattacgaa
     9001 tatctgcatc ggcgaactga tcgttaaaac tgcctggcac agcaattgcc cggctttctt
     9061 gtaacgcgct ttcccaccaa cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg
     9121 cccacaggcc gtcgagtttt ttgatttcac gggttggggt ttctacagga cgtaacataa
     9181 gggactcctc attaagataa aaatactggt caacctttaa tctgattagt taaccaattc
     9241 tgtgttgcac acagttatct gtgagaaacc catggaagag aaatggaaat aaaaagaact
     9301 gacaccgcga tcaaaaaacc aaaaactgca atacccacag cgtgatattg atcgcattaa
     9361 tctttaaaac ggcgttgaca gaagccataa aataagcaac cgaacgttcg ttcgagtata
     9421 ttgggaacga taaactctca tgacatatgg ctacggtgaa tatttttggg ggggaattct
     9481 ccgatcccgg ctagattgta ttttgtacag ggaaattaaa gtatgaaacg cgggcacagt
     9541 tatcaccagc ccgcgaaatg agaggatcag gatgcggtta agataccgcc aatcatcgcg
     9601 cgtaatccct gagcaaacga cgcttcacgc gccagatctt ccgcttcgat atcggccagt
     9661 acaccgtagg tcagatccag cagtaaacgt gaagtcattg acgtgttgat gtctgggctt
     9721 atctcgcctt tttgctgggc atcacgcatc cgctcggcaa caaattcagt gatcgtcata
     9781 tgcttatttt taagcatggc ggcgacctgt gggttacgca tcccttccgc cataatttcg
     9841 accaccagcg cccgttgccc aaaggcttca tgggtgagag agacaataga ctcgaccata
     9901 tagtcaacaa aatgaatccc ttcaatcggt tcccggaaac gggccagcgc cctctcctgg
     9961 tcctgtaaga taatcgcctg aatcaaggct tctttggaga tgaaatggtg atagagcgtc
    10021 ccgggactaa tggcgcagga tttacagatg gctttcatcg aggcactgtg aaatccattt
    10081 tctgaaaaaa tctctctggc agcattgagg atccgggtcc gtgtcggttg tgcttcagtc
    10141 tgcatgttat ccatcataaa accattatag ttagcgtgaa taactagttt accgcgttgt
    10201 caggtgagcg cctatcattg ctgttgaata gtgcgaaggc acactctatt cgtatacttt
    10261 tcactctgaa attggttaac caatagccgt gatttggctg ccagttattt agcagagacg
    10321 ttgatgagtt tatcccggta aacaacacgc acggttgttt accggtccgt tagtgtatta
    10381 attgagctcc tgtaccccac caccggagac ggtgagaatt tgtccgctta cccagctcgc
    10441 agcaggcgag caaaggaaca gcgctgcgtt agcaatatct tgcggttggc ccagacgtct
    10501 gatcggcgtg tgctgtaaca ttttttgttc aatttctggt gtaataacgg atttcagggc
    10561 atcggttaat attgcccccg gcgcaatgcc atttacccga atattttttt cacccaggtc
    10621 aaacgccata tttctgacca gatgactggc tgcagcttta gatgatgcat aggaagtcat
    10681 gtttatattt ttattttctg ccgccataga agtgatggtc agaataacgc caccgccatt
    10741 tttttccatt tctggcgcaa caagttgtga cagatggaaa aaagaaaaca cattcagttc
    10801 ataagcacgg cgaaaatccg ccattggcat atcaaacggt ttaggtccac cgccaccggc
    10861 gttgttaacc agaatatcaa ctttacccag cttactgata gcaaagtctg ccagtgcaga
    10921 gagttcctgt tcggaagtaa tatcacaacg gcaggcaaat gcctgaccac ccagttgttg
    10981 aatttcgtct acaacatggt tagctgcgtc ggcgttaata tcactgacca ccacagatgc
    11041 gccagctgtc gcgaatgtaa tggcgatttc tttaccaata cctgcacccg cacctgtgat
    11101 gatggcgcat tttccgtcga gtctcaggtt gtcagaatta aacacataaa cctcctgtga
    11161 acttcagtaa aaataaaagg caccgcctgt gtgccacatt ttttatcatt aaccttgagg
    11221 aaaggatact accaggtata gcagataaag agaggattat ttcgccgcaa tgagccgggc
    11281 gggaataata agattgcgtg aatgcgtctc ttcatgggtg attttttgca tcatgcgatc
    11341 cgcaagtgtg ataccaagtt cccgcgctgg cgtgctggcc caggtaacag gtatatcatc
    11401 aagtgtggtt ggtgtcgcat cggtaaatgc cgccagcgaa acctgttgct caaagtaacg
    11461 atcgactccg ctttccccgc tttgccgccc tgctttgagc aaaccaaacc atgcccccat
    11521 cgcaatagtt tcgttatagc aaaccacggc actgatggtc gggttatgac gtaaaagcgc
    11581 cgtgatagct tccgcggctt gcttctggct ggaagtgcac tccaacaccc aatcgctatg
    11641 aaacggcagg ccaaatttta gtagagttgc acaatagccc cccacccgtt ctgcacgggt
    11701 taatgaggaa ctttgccctc ccagccaggc gatccgctga tgcccattgc gaatgagatg
    11761 ctccgtcaac aactgtgcag cctgcatgtt gtccgggcga accgtatcaa catcatcaag
    11821 ataactggca cgggaagcga aaatcaccgg gatagctttt tcttctgcca tccgtcgcag
    11881 gtcatcgctg cttcctgcag ccccggcaat taccacacca tccacaccct gattcagtaa
    11941 cagtgaaaac cgctgtgcca gctgctcgcc gtctttaccg ccgtgaagca aaaaaaccat
    12001 ccgtccctgc gcttccagag cttccgtcaa tccggccgtc aattcggcgt aaaacggcgc
    12061 agataaatca cggacgatca aaccaatgac gccgctttgc ccgccgcgca gcgccgacgc
    12121 ctggcgattg cgcacaaatc ccagctcttc aatggcggcg ttaacgcgtt ctcctgtggc
    12181 ggtagagatt cgccctttgc cactaagcac cagcgaaacg gtacttaccg acacgcccgc
    12241 agccagcgca acatcatgaa tggttatttt tttggcggta gccatgcaga tgacctactc
    12301 cctgattatg tgacagataa aacgttttac cttttattta tcttatactc gctattatcg
    12361 ttgcgtaatg tgatttatgc ctcactaaaa tttgataaaa cgttttatct tctcgcgcaa
    12421 tttactgaat ccagattgtt ctctacgagg agtcgtttta tgacggcgaa aacagcaccg
    12481 aaagtcacgc tgtgggagtt cttccagcag ttaggcaaaa ccttcatgtt acccgtggca
    12541 ttattgtcgt tctgcggcat tatgctcggc attggtagtt ctcttagtag ccacgatgtc
    12601 atcaccctga tcccggtcct gggcaacccc gtgttgcagg ctatctttac ctggatgagt
    12661 aagattggct cgtttgcttt tagtttcctg ccagtcatgt tctgtatcgc catcccgctg
    12721 ggtctggcac gcgaaaacaa aggcgtagcg gcattcgctg gcttcgtcgg ttatgcggta
    12781 atgaacctcg cggtaaactt ctggttgacc aataaaggca ttctgccaac cacggacgcc
    12841 gcggttctga aagccaataa catccagagc attcttggga tccagtcgat cgacaccggt
    12901 atcctcggtg cggtgatcgc cggtattatc gtctggatgc tgcatgagcg attccataat
    12961 atccgcctgc cggatgcgct tgcattcttc ggcggtacgc gcttcgtacc aatcatctcc
    13021 tcgctggtga tgggccttgt cggcctggtg attccattag tctggccgat tttcgcgatg
    13081 ggtattagcg gcttgggcca tatgataaac agcgcgggtg atttcggacc gatgctgttt
    13141 ggtaccggtg aacgtctgct gttgccgttt ggtctgcatc acattctggt ggcattaatt
    13201 cgctttaccg acgcaggcgg cacgcaggaa gtctgcggtc aaaccgtcag cggcgcattg
    13261 accatcttcc aggcgcaatt gagttgcccg accactcacg gtttttctga aagcgccacg
    13321 cgtttccttt cgcaaggtaa aatgcctgcg tttctcggcg gtctgccagg tgcagcgtta
    13381 gctatgtatc actgcgcgcg cccggaaaat cgccataaaa ttaaaggtct gctgatttct
    13441 ggcctgatcg cctgcgtcgt tggcggcact accgaaccgc tggaattcct gttcctgttc
    13501 gtagcgccag ttctgtatgt catccacgcg ctgttaaccg gcctcggctt caccgtcatg
    13561 tctgtgctcg gcgtcaccat cggtaatacc gacggcaata tcatcgactt cgtggtgttc
    13621 ggtattttgc atggtctgtc aaccaagtgg tacatggtgc cagtggtggc ggcaatctgg
    13681 tttgtcgttt actacgtcat cttccgtttc gctatcaccc gcttcaatct gaaaaccccg
    13741 gggcgcgata gcgaagttgc cagctcaatc gaaaaagccg ttgccggtgc gccgggtaaa
    13801 tcaggttaca acgttcctgc aatcctcgaa gcattaggcg gtgccgacaa tattgtcagc
    13861 ctcgataact gcattacccg tctgcgtttg tctgtgaaag atatgtcgct tgttaatgtg
    13921 ctggcactga aggacaatcg ggcaattggc gtagtacaac ttaatcaaca taacctgcag
    13981 gttgttatcg ggccacaagt tcagtcagta aaagatgaaa tggccggtct gatgcatact
    14041 gtccaggcat aaggataaga tatgttcgat ttttcaaagg tcgtggatcg tcatggcaca
    14101 tggtgtacac agtgggatta tgtcgctgac cgtttcggca ctgctgacct gttaccgttc
    14161 acgatttcag acatggattt tgccactgcc ccctgcatta tcgaggcgct gaatcagcgc
    14221 ctgatgcacg gcgtatttgg ctacagccgc tggaaaaacg atgagtttct cgcggctatt
    14281 gcccactggt tttccaccca gcattacacc gccatcgatt ctcagacggt ggtgtatggc
    14341 ccttctgtca tctatatggt ttcagaactg attcgtcagt ggtctgaaac aggtgaaggc
    14401 gtggtgatcc acacacccgc ctatgacgca ttttacaagg ccattgaagg taaccagcgc
    14461 acagtaatgc ccgttgcttt agagaagcag gctgatggtt ggttttgcga tatgggcaag
    14521 ttggaagccg tgttggcgaa accagaatgt aaaattatgc tcctgtgtag cccacagaat
    14581 cctaccggga aagtgtggac gtgcgatgag ctggagatca tggctgacct atgcgagcgt
    14641 catggtgtgc gggttatttc cgatgaaatc catatggata tggtctgggg cgagcagccg
    14701 catattccct ggagtaatgt ggcgcgggga gactgggcgt tgctgacgtc gggctcgaaa
    14761 agtttcaata ttcccgcgct gaccggtgct tacgggatta tagaaaatag cagcagccgt
    14821 gatgcctatt tatcggcact gaaaggacgt gatgggcttt cttccccttc ggtactggcg
    14881 ttaactgccc atatcgccgc ctatcagcaa ggtgcaccgt ggctggatgc cttacgcgtc
    14941 tatctgaaag ataacctgac gtatatctca gataaaatga acgccgcgtt tcctgaactc
    15001 aactggcaga tcccgcaatc cacttatctg gcctggcttg atctacgtct gttgaatatt
    15061 gacgacaacg cgttgcaaaa agcgcttatc gagcaagaaa aagtcgcgat catgccgggg
    15121 tatacctatg gtgaagaagg tcgtggtttt gtccgtctca atgccggctg cccacgttcg
    15181 aaactggaaa aaggtgtggc tggattaata aacgccatcc gcgctgttcg ctaaccccaa
    15241 ttgcgcaacg taaaaaatcg ttgcgcaatc gtggattttt accctgcttt gtttttataa
    15301 tggtgcgcac ttttatattc agaaaaagag tgcgaccatg attgatacca ccctgccatt
    15361 aactgatatc catcgccacc ttgatggcaa cattcgtccc cagaccattc ttgaacttgg
    15421 ccgccagtat aatatctcgc ttcctgcaca atccctggaa acactgattc cccacgttca
    15481 ggtcattgcc aacgaacccg atctggtgag ctttctgacc aaacttgact ggggcgttaa
    15541 agttctcgcc tctcttgatg cctgtcgccg cgtggcattt gaaaacattg aagatgcagc
    15601 ccgtcacggc ctgcactatg tcgagctgcg tttttcacca ggctacatgg caatggcaca
    15661 tcagctgcct gtagcgggtg ttgtcgaagc ggtgatcgat ggcgtacgtg aaggttgccg
    15721 cacctttggt gtgcaggcga agcttatcgg cattatgagc cggaccttcg gcgaagccgc
    15781 ctgtcagcaa gagctggagg cctttttagc ccaccgtgac cagattaccg cacttgattt
    15841 agccggtgat gaacttggtt tcccgggaag tctgttcctt tctcacttca accgcgcgcg
    15901 tgatgcgggc tggcatatta ccgtccatgc aggcgaagct gccgggccgg aaagcatctg
    15961 gcaggcgatt cgtgaactgg gtgcggagcg tattggacat ggcgtaaaag ccattgaaga
    16021 tcgggcgctg atggattttc tcgccgagca acaaattggt attgaatcct gtctgacctc
    16081 caatattcag accagcaccg tagcagagct ggctgcacat ccgctgaaaa cgttccttga
    16141 gcatggcatt cgtgccagca ttaacactga cgatcccggc gtacagggag tggatatcat
    16201 tcacgaatat accgttgccg cgccagctgc tgggttatcc cgcgagcaaa tccgccaggc
    16261 acagattaat ggtctggaaa tggctttcct cagcgctgag gaaaaacgcg cactgcgaga
    16321 aaaagtcgcc gcgaagtaac aaaatggatg gtgcaaattg caccatccat ttttcatgca
    16381 aggcacaaag tcgcgcgatg tttggcggat tcgatgccca gctcaatcaa ctccattacc
    16441 tggattgcct ggcttgccgg aaccggattt tcaccatcgc catttaacgc atcacgaata
    16501 gccgcatagt aagccggata attcccaggt accgtcaaca gcgtttcttc gacacgttcc
    16561 tcaccttcca cgcgggtaag tacgccatca cgcatatcgt agccccagtc ttcctgcggc
    16621 agacgctcgc catttttcag acgttcttcc tgtggatcga ggccatattt cacataactg
    16681 cctcgggatc catgcacgat ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt
    16741 aaaatgactc gccgctgtgg ataggacaag atggcgtgga aataatcggt cgactgcgct
    16801 ccgggccgta actgtgccaa atctaccgtc atgctgaccg gtaaaccaaa aagcgtaatg
    16861 gcctgatcaa gaagatgtgg tgctaaatcg taccagatac cgctgcctgg gccgccctgt
    16921 tcacgccaac gatcgcgcac ctgcggacgg aagcggtcaa aatgagactc aaagtaagca
    16981 acttcaccca gcacgccttc cgcgagtaaa ccttttagcg tcaagaaatc gctatcccag
    17041 cgacggttat ggaatacaga cagcacacgc cccaggcttt ttgccagcgc atccagctct
    17101 cgcgcttgtg acagtgtcac ggtaaagggt ttatcaacga ccacatgttt acccgcctca
    17161 agcgccgctt tggctaacgg gaaatgggta tcgttgggtg taggaatgac aatcaggtct
    17221 atgttgggat cgttaaacag atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg
    17281 gcttttactt ttgtttcatc actgctggag attaccgcca gttccagccc gggcgtgccc
    17341 gcaatcaggg gcgcatggaa ggttttgctc gcataaccat acccaatcaa cccaacacgg
    17401 atgttgtcgc tcatgtcatt gcctctcatt gaagtatgat ggctatttga cactatcctt
    17461 tacccacgct caacagttta ataacctgcc agcaataagg gatgttgttt aacttaagtc
    17521 aaaaaaatag cgaattttcc aacgacaaaa gctaaatatc gcaaaaacct cagtaaaaat
    17581 cttgctggag ctattattgc taagtaacat ttaccccctg aagttaatgg atcaatcaag
    17641 agagatgtgg gctgtaatga atcgtcttat tgaattaaca ggttggatcg ttcttgtcgt
    17701 ttcagtcatt cttcttggcg tggcgagtca cattgacaac tatcagccac ctgaacagag
    17761 tgcttcggta caacacaagt aagctctgca cttgtggagc gacatgctgc ccgtccgggt
    17821 gcatgttttc acttgtcgga tattaaacca ggaatttatt atcttgttcg atgttgttgg
    17881 tgattgtcag ggatagtaaa gcgcgttact cttttcagag cagtttttca tccctgaata
    17941 tttgccatca gcgcattctt attattgtaa taacacaagc gttcactttt ggtgaaattt
    18001 tatcatcact aataatacct acccctttgg tattaattat tttatggacc ttttatgact
    18061 gttcaggact acttattaaa atttcgcaaa atcagttcac tcgaaagtct ggaaaaactc
    18121 tacgaccatc ttaattacac cctgacggac gatcaggaac tgatcaatat gtatcgtgct
    18181 gccgatcacc gtcgcgcaga gctggtttct ggcgggcgtt tgtttgacct cggccaggta
    18241 ccgaagtccg tctggcacta tgtccaataa agaaagtagc gatcggttat aaaagcctta
    18301 taataacgcc cctaaaatta gatttgcctg tttgcgtcgc tattctggag aacctatgac
    18361 caccacaacg ccacaacgta ttggaggctg gttgcttggc ccgttggcct ggcttttagt
    18421 cgctctgttg agtacgacgc tggcgttgtt gctgtatacc gctgcgttat cttctccgca
    18481 aacatttcaa acgctcggcg gacaagcact gacgacacaa atcttatggg gcgtttcttt
    18541 tattaccgct atcgccatgt ggtattacac actgtggttg accatcgctt tctttaaacg
    18601 tcggcgttgt gttcctaaac actatattat ctggctgctt atttccgtat tgctggcagt
    18661 aaaagcattc gctttttcac ccgtggaaga tggcatagcc gttcgccagt tgctgtttac
    18721 tttgttggca accgcattga tcgtccccta tttcaaacgc tcgtcgcggg taaaagcgac
    18781 gtttgtgaat ccgtaataac cttacagtta acctgttgtc gcctgctctg gattaacgga
    18841 taataggcgg cttttttatt tcaggccgaa aaatgactga ctacctgtta ctgtttgtcg
    18901 gaactgtact ggtcaataac tttgtactgg tcaagtttct cggtctctgt ccgtttatgg
    18961 gggtttccaa aaaactggaa accgcgatgg gcatggggct ggcaacaacg tttgtgatga
    19021 cgctggcgtc tatttgcgcc tggcttatcg atacgtggat tttgatccca cttaatctga
    19081 tttacctgcg caccctggca tttattctgg tgattgctgt ggtcgtgcag ttcaccgaga
    19141 tggtggtgcg caaaaccagc ccggtgcttt accgtctgct ggggattttt ttgccgctta
    19201 tcaccaccaa ctgtgcagtg ctcggcgtgg cgttgctgaa tatcaatctc gggcacaatt
    19261 tcttgcagtc ggcgctgtac ggtttttccg ccgctatcgg tttctcgctg gtgatggtgc
    19321 tcttcgccgc catccgcgaa cgccttgctg tggctgatgt cccggcacct tttcgcggtc
    19381 atgccattgc gttaattacc gcaggtctta tgtctctggc ctttatgggc tttagtggtt
    19441 tggtgaagtt gtaatgaatg ctatctggat tgccgttgcc gccgtgagcc tgctgggcct
    19501 ggcgtttggc gccattctgg gttatgcctc ccgccgtttt gcggtggaag acgatccggt
    19561 cgttgagaaa attgacgaaa tcttaccgca gagccagtgt ggtcagtgcg gttatcccgg
    19621 ctgtcgcccc tacgcggaag ccatcagctg taacggtgaa aaaatcaacc gttgcgcccc
    19681 aggtggcgaa gctgtgatgc taaaaattgc cgagttgctt aatgtcgagc cgcagccgct
    19741 ggatggcgaa gcgcaagaga taacgcctgc gcggatggtg gcggttattg atgaaaataa
    19801 ctgtattggc tgcactaaat gtattcaggc gtgtccggta gacgccatcg ttggcgctac
    19861 ccgtgccatg catacggtaa tgagtgatct ctgtacgggc tgcaatttat gtgttgatcc
    19921 gtgcccgacg cactgcatct cgttgcaacc ggtcgcagaa acacctgact cctggaaatg
    19981 ggatctgaac accattcccg tgcgtatcat tcccgtggaa caccatgctt aagttattct
    20041 ctgcattcag aaaaaataaa atctgggatt tcaacggcgg catccatcca ccggagatga
    20101 aaacccagtc caacggtaca cccctgcgcc aggtaccact ggcgcagcgt tttgttattc
    20161 cactgaaaca gcatattggc gctgaaggtg agttgtgcgt tagcgtcggc gataaagtat
    20221 tgcgcggcca gccgcttacc cgtggtcgcg gcaaaatgct gcctgttcac gcgcccacct
    20281 cgggtaccgt tacggctatt gcgccccact ctacggctca tccttcagct ttagctgaat
    20341 taagcgtgat tattgatgcc gatggtgaag actgctggat cccgcgcgac ggctgggccg
    20401 attatcgcac tcgcagtcgc gaagagttaa tcgagcgcat acatcagttt ggtgttgccg
    20461 ggctgggcgg tgcaggattc ccgacaggcg ttaaattgca gggtggcgga gataagattg
    20521 aaacgttgat tatcaacgcg gctgagtgcg agccgtacat taccgccgat gaccgtttga
    20581 tgcaggattg cgcggctcag gtcgtagagg gtattcgcat tcttgcgcat attctgcagc
    20641 cacgcgaaat tcttatcggc attgaagata acaaaccgca ggcgatttcc atgctgcgcg
    20701 cggtgctggc ggactctaac gatatttctc tgcgggtgat tccaaccaaa tatccttctg
    20761 gcggtgctaa acaattaacc tacattctga ccgggaagca ggttccacat ggcgggcgtt
    20821 catccgatat cggcgtatta atgcaaaacg tcggcactgc ttatgcagtg aaacgtgccg
    20881 ttattgatgg cgagccgatt accgagcgtg ttgtaaccct gactggcgaa gcaatcgctc
    20941 gcccgggcaa cgtctgggca cggctgggga cgccagtgcg tcatttattg aatgatgccg
    21001 gattctgccc ctctgccgat caaatggtga ttatgggtgg cccgctaatg ggctttacct
    21061 tgccatggct ggatgtcccg gtcgtaaaga ttaccaactg tctgttggct ccctctgcca
    21121 atgaacttgg cgaaccacag gaagaacaaa gctgcatccg gtgtagcgcc tgtgctgacg
    21181 cctgccctgc ggatcttttg ccgcaacagt tgtactggtt cagcaaaggt cagcaacacg
    21241 ataaagctac cacgcataac attgctgatt gcattgaatg tggggcttgc gcgtgggttt
    21301 gcccgagcaa tattcccctg gtgcaatatt tccgtcagga aaaagctgaa attgcggcta
    21361 ttcgtcagga agaaaagcgc gccgcagaag ccaaagcgcg tttcgaagcg cgccaggctc
    21421 gtctggagcg cgaaaaagcg gctcgccttg aacgacataa gagcgcagcc gttcaacctg
    21481 cagccaaaga taaagatgcg attgctgccg ctctggcgcg ggtgaaagag aaacaggccc
    21541 aggctacaca gcctattgtg attaaagcgg gcgaacgccc ggataacagt gcaattattg
    21601 cagcacggga agcccgtaaa gcgcaagcca gagcgaaaca ggcagaactg cagcaaacta
    21661 acgacgcagc aaccgttgct gatccacgta aaactgccgt tgaagcagct atcgcccgcg
    21721 ccaaagcgcg caagctggaa cagcaacagg
//
LOCUS       DAZFWL020000075        21674 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFWL020000075 DAZFWL020000000
VERSION     DAZFWL020000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.075"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1010)
                     /gene="malZ"
                     /locus_tag="ACHKNP_RS14880"
                     /old_locus_tag="pgaptmp_002976"
     CDS             complement(<1..1010)
                     /gene="malZ"
                     /locus_tag="ACHKNP_RS14880"
                     /old_locus_tag="pgaptmp_002976"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HGU4705105.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFDGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RL"
     gene            complement(1166..2539)
                     /gene="proY"
                     /locus_tag="ACHKNP_RS14885"
                     /old_locus_tag="pgaptmp_002977"
     CDS             complement(1166..2539)
                     /gene="proY"
                     /locus_tag="ACHKNP_RS14885"
                     /old_locus_tag="pgaptmp_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HGU4705106.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(2615..3934)
                     /gene="brnQ"
                     /locus_tag="ACHKNP_RS14890"
                     /old_locus_tag="pgaptmp_002978"
     CDS             complement(2615..3934)
                     /gene="brnQ"
                     /locus_tag="ACHKNP_RS14890"
                     /old_locus_tag="pgaptmp_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HGU4705107.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(4341..5636)
                     /gene="phoR"
                     /locus_tag="ACHKNP_RS14895"
                     /old_locus_tag="pgaptmp_002979"
     CDS             complement(4341..5636)
                     /gene="phoR"
                     /locus_tag="ACHKNP_RS14895"
                     /old_locus_tag="pgaptmp_002979"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HGU4705108.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWLRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(5694..6383)
                     /gene="phoB"
                     /locus_tag="ACHKNP_RS14900"
                     /old_locus_tag="pgaptmp_002980"
     CDS             complement(5694..6383)
                     /gene="phoB"
                     /locus_tag="ACHKNP_RS14900"
                     /old_locus_tag="pgaptmp_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HGU4705109.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            6573..7775
                     /gene="sbcD"
                     /locus_tag="ACHKNP_RS14905"
                     /old_locus_tag="pgaptmp_002981"
     CDS             6573..7775
                     /gene="sbcD"
                     /locus_tag="ACHKNP_RS14905"
                     /old_locus_tag="pgaptmp_002981"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HGU4705110.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            7772..10915
                     /gene="sbcC"
                     /locus_tag="ACHKNP_RS14910"
                     /old_locus_tag="pgaptmp_002982"
     CDS             7772..10915
                     /gene="sbcC"
                     /locus_tag="ACHKNP_RS14910"
                     /old_locus_tag="pgaptmp_002982"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HGU4705111.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQRIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASETQEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            11041..12225
                     /gene="araJ"
                     /locus_tag="ACHKNP_RS14915"
                     /old_locus_tag="pgaptmp_002983"
     CDS             11041..12225
                     /gene="araJ"
                     /locus_tag="ACHKNP_RS14915"
                     /old_locus_tag="pgaptmp_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HGU4705112.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCCGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(12368..13276)
                     /gene="mak"
                     /locus_tag="ACHKNP_RS14920"
                     /old_locus_tag="pgaptmp_002984"
     CDS             complement(12368..13276)
                     /gene="mak"
                     /locus_tag="ACHKNP_RS14920"
                     /old_locus_tag="pgaptmp_002984"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HGU4705113.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            13401..14312
                     /gene="rdgC"
                     /locus_tag="ACHKNP_RS14925"
                     /old_locus_tag="pgaptmp_002985"
     CDS             13401..14312
                     /gene="rdgC"
                     /locus_tag="ACHKNP_RS14925"
                     /old_locus_tag="pgaptmp_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HGU4705114.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(14390..14473)
                     /locus_tag="ACHKNP_RS14930"
                     /old_locus_tag="pgaptmp_002986"
     CDS             complement(14390..14473)
                     /locus_tag="ACHKNP_RS14930"
                     /old_locus_tag="pgaptmp_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705115.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(14470..14811)
                     /locus_tag="ACHKNP_RS14935"
                     /old_locus_tag="pgaptmp_002987"
                     /pseudo
     CDS             complement(14470..14811)
                     /locus_tag="ACHKNP_RS14935"
                     /old_locus_tag="pgaptmp_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(14959..15243)
                     /gene="ppnP"
                     /locus_tag="ACHKNP_RS14940"
                     /old_locus_tag="pgaptmp_002988"
     CDS             complement(14959..15243)
                     /gene="ppnP"
                     /locus_tag="ACHKNP_RS14940"
                     /old_locus_tag="pgaptmp_002988"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HGU4705116.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(15315..15992)
                     /gene="aroM"
                     /locus_tag="ACHKNP_RS14945"
                     /old_locus_tag="pgaptmp_002989"
     CDS             complement(15315..15992)
                     /gene="aroM"
                     /locus_tag="ACHKNP_RS14945"
                     /old_locus_tag="pgaptmp_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HGU4705117.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(16250..16441)
                     /gene="yaiA"
                     /locus_tag="ACHKNP_RS14950"
                     /old_locus_tag="pgaptmp_002990"
     CDS             complement(16250..16441)
                     /gene="yaiA"
                     /locus_tag="ACHKNP_RS14950"
                     /old_locus_tag="pgaptmp_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HGU4705118.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(16491..17015)
                     /gene="aroL"
                     /locus_tag="ACHKNP_RS14955"
                     /old_locus_tag="pgaptmp_002991"
     CDS             complement(16491..17015)
                     /gene="aroL"
                     /locus_tag="ACHKNP_RS14955"
                     /old_locus_tag="pgaptmp_002991"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HGU4705119.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(17198..17656)
                     /gene="yaiI"
                     /locus_tag="ACHKNP_RS14960"
                     /old_locus_tag="pgaptmp_002992"
     CDS             complement(17198..17656)
                     /gene="yaiI"
                     /locus_tag="ACHKNP_RS14960"
                     /old_locus_tag="pgaptmp_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HGU4705120.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            17776..18585
                     /gene="proC"
                     /locus_tag="ACHKNP_RS14965"
                     /old_locus_tag="pgaptmp_002993"
     CDS             17776..18585
                     /gene="proC"
                     /locus_tag="ACHKNP_RS14965"
                     /old_locus_tag="pgaptmp_002993"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGU4705121.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(18602..19717)
                     /gene="adrA"
                     /locus_tag="ACHKNP_RS14970"
                     /old_locus_tag="pgaptmp_002994"
                     /gene_synonym="dgcC"
     CDS             complement(18602..19717)
                     /gene="adrA"
                     /locus_tag="ACHKNP_RS14970"
                     /old_locus_tag="pgaptmp_002994"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HGU4705122.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(19819..20139)
                     /gene="psiF"
                     /locus_tag="ACHKNP_RS14975"
                     /old_locus_tag="pgaptmp_002995"
     CDS             complement(19819..20139)
                     /gene="psiF"
                     /locus_tag="ACHKNP_RS14975"
                     /old_locus_tag="pgaptmp_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HGU4705123.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(20258..21673)
                     /gene="phoA"
                     /locus_tag="ACHKNP_RS14980"
                     /old_locus_tag="pgaptmp_002996"
     CDS             complement(20258..21673)
                     /gene="phoA"
                     /locus_tag="ACHKNP_RS14980"
                     /old_locus_tag="pgaptmp_002996"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HGU4705124.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITASGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
BASE COUNT         5419 a         5838 c         5450 g         4967 t
ORIGIN      
        1 tccagccgcc agccgtccat actccacggc gctttcagcc aatggcggac aatactgtct
       61 tccccgcgat aaatttcatt caccagactt tccgactgat aatccagctt cggcaagctg
      121 gcatagccaa gccagtcgag cgccgtgcca tcatcactaa acgagtacca gtcgcgccag
      181 ggcgattcag ggttgtgaca agctcccccc gtgccacgat tgtgcctgtc aaaccaggca
      241 tgggaatcgc cactgtggtt aaacacgcca tccagcacca gccgcattcc cagctgctgc
      301 gtattgtggc gtaagcgcag caacgcccca tcaccgtcaa actgcggatc gacatggcga
      361 taatcctcgg tatcgtattt atgtacgctg ggagctttaa acaccggatt gagatacagc
      421 gccgtcacgc caagcttttt cagatacggc agtttttcgc ttatcccgtc cagatcgccg
      481 ccatagaacg ttgatccgcc cgcctgcgcc gtgaccggtt catcccagtc acgcaagatg
      541 atctcttgtc cggctgcatg atggtaatag acatgatcct gttcagcttc acgaggaaga
      601 ctacgcgcaa aacgatcagg gaagatctga taaaaaatct gatccgcagc ccattgtggg
      661 ccgatatccg gtacatcgac ggcaaactgc tccagtcgtg ccggcggcat tcggctgaag
      721 ccctgcggtg taaaccaacg ctggcgatcg tgccacagca gtttgaaact gtaacgccgc
      781 cggggttgtc cgctggagag atcaatcgcc gcacgccatg cggtgacgcc aggctgctgc
      841 tgactgcgct gcttatgcat cgatactgac atttcttcgt tatcgtgttc tgtacgcagc
      901 ataatgcgct gcggtgggtc ttcgcccgtc agccacagtg taatgagcag ttgatctttg
      961 ctttgtttaa caaatggggg caccggcagg tgccatgcat ttaacatcat aattcccctg
     1021 tgatgaaaat ctgatcacct tgccatagcc taatgcagga tcactcatca tctgaaggat
     1081 atttggggga ggaggacggg ggcggataac tttcggaata gccggatcag gatttcctcc
     1141 tgaaccggct tacagaacgc aagtattatt ggttttcagc cagctgacga tcgtggcggc
     1201 gtttaaacat ccagccaatc aacagcacaa caatccacgc gaaaccgaca tacagcgaga
     1261 tacgcgtatc cgggtgataa ccaatcaacc cgataataaa gagcaggaaa atcagcccgc
     1321 cgatggtcgt tgctacccca cccggcactt taaatttcag cgccttaact tcttctggcg
     1381 gcaaacggcg acggaaggca atttgcgaca gcaggatcat aatccacacc cacaccgtgg
     1441 cgaaggttgc cagcgaagcg atcaccagga agacgttttc cggcatgatg tagttcagat
     1501 acaccgcaaa cagcagcgcg gtagtcatca ccagcaccgt aacccacgga ataccgcgac
     1561 gcgacgtttt gctgaaaatt ttcggcgcgc tgccctgctc tgccataccg tggagcatac
     1621 ggcctacgcc aaatacgtca ctgttaattg ccgacagcga agcagtcagc acaacaaagt
     1681 taagaatgct ggcggcaaag gtaatgccca tatgctggaa cgtcagcacg aacgggctac
     1741 cggcagtgcc aacctgattc cacgggtaga tagacataat gacgaacagc gtaccgacgt
     1801 agaacaccag aatacgcatc ggcacggagt taatcgcacg cggtatcgat ttctcaggat
     1861 ctttcgcttc accggcggta atcccgataa tttcgatccc accgtaagca aacatcacca
     1921 tttgcaacga catcaccatc ccaagccagc cgttactgaa gaagccgccg ttgctccaca
     1981 ggttatgaat accggtcggt tgcccgccgt tgccaatccc ccagatgatg atgccgaaac
     2041 cggcgacaat catgatgatg atggtggcga ctttaaagaa cgagaaccag aattccagct
     2101 caccgaagac cttcacgctc atcaggttta cggcgcaaat gatcagcacc acgctcagta
     2161 cccaaatcca gtgcggcacc gtcgggaacc agacacccat atagatacca aaagcggtca
     2221 catcggcgat ggcgacaata aggatttcaa agcaataggt ccagccggta atgtaacctg
     2281 ccagcgggcc gaggttttcc tgcgcataac gcgagaaaga gctggctgcc gggttatgta
     2341 ccgacatttc ccccagcgca cgcataatga tatacgccgc gataccaccg ataatatagg
     2401 ccaacaacac gctcggaccg gccattttga tggcgtctgc cgaaccgtaa aacagcccgg
     2461 tgccaattgc tgaacccagt gccataaagc gtatgtggcg ggtacttagc ccacgcttta
     2521 gcttgttctt actttccatc aattcctaac ccatcaacac aataaaaaac cacggggcat
     2581 cgcagccccg tggttaaaac aaatgttcag tgatttagtg agcgctggag gtcacctgac
     2641 gacctgccgc gcgatcccag ataatggcca gaaccaccat caccactgtt ggcattaacc
     2701 acgccagacc ttgttcggcc agcggtaaac gctgcgccca ggacggtaag atatcgctga
     2761 atgcagatgc tttgatcccg tcgagaatac caaaaagcag gctgataaac atcggcggag
     2821 caatcacgcg ggacgaatta tgccaccatg agcgtgtaaa acttaataca accagtgcga
     2881 tacacggcgg ataaatagcg gtcagcaccg gtacggagat ctggatcagc tggcttaagc
     2941 cgaggttaga aaccaccatc gagaagccgc cgaggataaa caccagcgta cgataagaga
     3001 gcggtacgta ctgggcaaag aattctgcac aagcacaggt caggccaact gccgttacca
     3061 ggcaggcgat gaagattaac gccgccagca ggaagctacc gccgccgcca aaggtgtgct
     3121 gaacgtaagc atgcagaata gcagcgccgt ttgcagactg atcgaccagc gacgcgctgt
     3181 ctgaacccag acggaacagc gccaggtaca gcagagtcag accaacaccc gccatcaggc
     3241 cagcccagac ggtataacgg gtcagcagac gcgcttcggt aacgccacga gaacgcgccg
     3301 cgttaacaat aacgatacca aacaccattg cgcccagcgt atccatggtc agatagccgt
     3361 taacgaagcc gttagaaaac gcagcgtttt gataagcctc agtcgccgtg ctgatagaac
     3421 ccgccggcca gacaatcgcg gcaacagaca ggatgaccag cgcgataatt ttcagcggcg
     3481 caaggaagtt gcccacggta tccagcagct tgcccggata gagcgaaacc agaataacga
     3541 tagcgaaata gaccaggctg tagataaaca gcggcagcgc ggaatcaccc gtcagcggcg
     3601 caatccccac ttcaaaggaa acggtagctg tacgcggcgt agcgaaaagc ggccccaccg
     3661 ccaggtaaca aaccgttgcc agcagtacgc cagcgacttt accgattggc gtgctgaggc
     3721 tgtcaacacc gccgccaact tttgccagcg ccactaccgt taataccggc aggccaacgg
     3781 cagtaatgag gaagccgaat gccgcagtcc agacgtgttc gcctgcctgt aagccgacca
     3841 ttggagggaa aataatgtta cctgcgccga cgaacaacgc aaatgtcata aagcccagag
     3901 cgatgatatc gcgcgatctt aattgatggg tcataaatca tactgcctgt ggatgtggtg
     3961 ttgtaacgtt aaatttttgt gccaatccct ggggatgata cagcggtata tttaaacaat
     4021 tttagcggga aatactccca atcaggcgcg gcgaagaata gtttttcgat ttaccgcgtt
     4081 aatgcagtca gtatgacagc acctgaagcg caatttaaac gcttataacg ttttaaagca
     4141 aggagggatc taaaaaccag aggtatatag cgataagaaa tcaccaacca gtataaaaca
     4201 ctatttccgt gaaaaagaca tggctaatca tgcgaacaaa aaatcaccaa ccgttgtaaa
     4261 atctaacgcc cgtattttga ctgcaaagaa aataagccag catcggctgg cttatggcaa
     4321 taaaagatga caaaggcgga ttaatcgctg tttttggcaa ttaaacgttc cgggataaca
     4381 aaactgaaac gtgttccttt tcctactgta ctctcaatat tcaggcgact ttcgtgatga
     4441 ttcaccgcat gtttcacgat cgctaaccct aatccgctac cgccggtttg ccgggaacgc
     4501 gctttatcaa cgcgataaaa acgctcggtc aggcgcggaa tatgctccgg tgcaatgccc
     4561 ggtccgttat cttcaacgct aaattcggca ccgtgcggca ctcgcagcca gcgtacggtg
     4621 atatgcgtgc cttccggcgt atgattcacg gcgttataga ccaggttcga aatcgcactg
     4681 cgtagctgat cttcgttgcc agacaccttg aggccgttat ctatctcaaa ggtaaatgtc
     4741 tgttttttct gactcagagt ctgagcctcg cgctcaacaa cgcgcagcat catcggcaca
     4801 tcaacctttt cattgagcaa ttgcgtcggc gcggcttcaa ttttcgacag cgttagcaac
     4861 tgcttcacca gcccttccat ccgctgggtc tgctcgcgca tggtgtgcaa cgctttttcg
     4921 cgtaccgcgc cttccagcgg ctgctcatcc atcatctcca ggtaaccctg taacacggtc
     4981 aatggcgtac gtaactcatg gctcacgttg gcaaaaaagt tacgccgcgc cccttccagt
     5041 tgatgcattt gcgtgacatc acgcgccacc atcagcaact gtttgtgggt ataaggcatg
     5101 acgcgaattt ccagatgccg cccggtgttg agcaccagat tgagcgggcg agaaaaatca
     5161 cgcgttttca gatattgcgt aaactccggg taacgcagca ggttaaggat gttctgcccg
     5221 ttatcttccg gccagcgcaa accaagaatt tgttgcgcca gaccgttaca ccagaaaata
     5281 ccgccctctt ccgtggtcag caccactgcg tcgggcagcg actccgcgcc gctacgaaag
     5341 cgtttaatca gattgcccag ttcacggcgg cgttttttat ttcgcagctg catctggtgt
     5401 aagccgtata gtagcggttc ccagctacca cgccccggtg gcggggtcat actacgatcc
     5461 acccacagcc accatgaaag gcgcaataaa ttccagaaat gccagataag cagtcccgtt
     5521 accgatgcca gcaaaaacca gggcaggtaa ccaaaaaatg cacccaggat gaaagccggg
     5581 aggcagcaaa gtagcagctc cagcaccagc cttttccacg acagccgttc cagcacgtaa
     5641 gatactccag ttaagaaatc ataagccctg ctctgcgtcc gatgagcaag gcgttaaaag
     5701 cgggttgaaa aacgatatcc tgtaccgcgc acggtctgca ccatgcggtc atgcccgccg
     5761 ggctccagtg ctttacgcag gcgacgaatg tggacatcga ccgtgcggtc ttccacatac
     5821 acgttagttc cccagacgtg gtttaacagc tgctcgcggc tgtacacgcg ctcaggatgc
     5881 gtcataaaaa agtgcagcag tttaaattct gtcggcccca tctccagcgg ctcttcgccc
     5941 gccatcactc ggtgagatgt cgggtcgaga cttaatccct gcatctcaat cacctcttcc
     6001 accgccattg gcgaaatacg gcgcattacc gctttgattc gcgccaccag ctccttcggc
     6061 gaaaacggct tggtgatata gtcatccgcg ccggtttcaa ggccgcgcac gcgatcttct
     6121 tcttcccctc tggcggtcaa catcaccact ggaatatccc gggtcatcga ctcgcgcttg
     6181 aggtgtttga tgaactggat accggagccg ccaggtaaca tccagtcaag gagaattaaa
     6241 tccggccagg gttcattcag ttgattcaca gcactgtcat aatcttccgc ttcgaccggc
     6301 tgaaagccat tttgttcgag cacgaagcag accatttcgc gaattggagc ttcatcttct
     6361 acgaccagaa tacgtctcgc catgatttgc cctgttgtaa taaataggtt gcgatcatta
     6421 atgcgacgtc attatgcgtc agatttatga cagatttatg aaaagctcgt cgcacatatc
     6481 ttcaggttat tgatttccgt ggcgcagaaa aaagcaaatg gcacatctgt ttgggtataa
     6541 tcgcgcccat gctttttcgc cagggaaccg ttatgcgcat ccttcacacc tcagactggc
     6601 atctcggcca gaacttctac agtaaaagcc gcgaagctga acatcaggct tttcttgact
     6661 ggctgctgga gacagcacaa acccatcagg tggatgcgat tattgttgcc ggtgatgttt
     6721 tcgataccgg ctcgccgccc agttacgccc gcacgttata caaccgtttt gttgtcaatt
     6781 tacagcaaac tggctgtcat ctggtggtac tggcaggaaa ccatgactcg gtcgccacgc
     6841 tgaatgaatc gcgcgatatc atggcgttcc tcaatactac cgtggtcgcc agcgccggac
     6901 atgcgccgca aatcttgcct cgtcgcgacg ggacgccagg cgcagtgctg tgccccattc
     6961 cgtttttacg tccacgtgac attattacca gccaggcggg gcttaacggt attgaaaaac
     7021 agcagcattt actggcagcg attaccgatt attaccaaca acactatgcc gatgcctgca
     7081 aactgcgcgg cgatcagcct ctgcccatca tcgccacggg acatttaacg accgtggggg
     7141 ccagtaaaag tgacgccgtg cgtgacattt atattggcac gctggacgcg tttccggcac
     7201 aaaactttcc accagccgac tacatcgcgc tcgggcatat tcaccgcgca cagattattg
     7261 gcggcatgga acatgttcgc tattgcggct cccccattcc actgagtttt gatgaatgcg
     7321 gtaagagtaa atatgtccat ctggtgacat tttcaaacgg caaattagag agcgtggaaa
     7381 acctgaacgt accggtaacg caacccatgg cagtgctgaa aggcgatctg gcgtcgatta
     7441 ccgcacagct ggaacagtgg cgcgatgtat cgcaggagcc acctgtctgg ctggatatcg
     7501 aaatcactac tgatgagtat ctgcatgata ttcagcgcaa aatccaggca ttaaccgaat
     7561 cattgcctgt cgaagtattg ctggtacgtc ggagtcgtga acagcgcgag cgtgtgttag
     7621 ccagccaaca gcgtgaaacc ctcagcgaac tcagcgtcga agaggtgttc aatcgccgtc
     7681 tggcactgga agaactggat gaatcgcagc agcaacgtct gcagcatctt ttcaccacga
     7741 cgttgcatac cctcgccgga gaacacgaag catgaaaatt ctcagcctgc gcctgaaaaa
     7801 cctgaactca ttaaaaggcg aatggaagat tgatttcacc cgcgagccgt tcgccagcaa
     7861 cgggctgttt gctattaccg gcccaacagg tgcggggaaa accaccctgc tggacgccat
     7921 ttgtctggcg ctgtatcatg aaactccgcg tctctctaac gtttcacaat cgcaaaatga
     7981 tctcatgacc cgcgataccg ccgaatgtct ggcggaggtg gagtttgaag tgaaaggtga
     8041 agcgtaccgt gcattctgga gccagaatcg ggcgcgtaac caacccgacg gtaatttgca
     8101 ggtgccacgc gtagagctgg cgcgctgcgc cgacggcaaa attctcgccg acaaagtgaa
     8161 agataagctg gaactgacag cgacgttaac cgggctggat tacgggcgct tcacccgttc
     8221 gatgctgctt tcgcaggggc aatttgctgc cttcctgaat gccaaaccca aagaacgcgc
     8281 ggaattgctc gaggagttaa ccggcactga aatctacggg caaatctcgg cgatggtttt
     8341 tgagcagcac aaatcggccc gcacagagct ggagaagctg caagcgcagg ccagcggcgt
     8401 cgcgttgctc acgccggaac aagtgcaatc gctgacagcg agtttgcagg tacttactga
     8461 cgaagaaaaa cagttactta ccgcgcagca gcaagaacaa caatcgctaa actggttaac
     8521 gcgtcaggac gaattgcagc aagaagccag ccgccgtcag caggccttgc aacaggtgtt
     8581 agccgaagaa gaaaaagcgc aacctcaact ggcggcgctt agtctggcac aaccggcacg
     8641 aaatcttcgt ccacactggg aacgcatcgc agaacacagc gcggcgctgg cgcatattcg
     8701 ccagcggatt gaagaagtaa atactcgctt acagagcaca atggcgcttc gcgcgagcat
     8761 tcgccaccac gcggcgaagc agtcagcaga attacagcag cagcaacaaa gcctgaatac
     8821 ctggttacag gaacacgacc gcttccgtca gtggaacaac gaactggcgg gttggcgtgc
     8881 gcagttctcc caacaaacca gcgatcgcga gcatctgcgg caatggcagc aacagttaac
     8941 ccatgctgag caaaaactta atgcgcttgc ggcgatcacg ttgacgttaa ccgccgatga
     9001 agttgctacc gccctggcgc aacatgctga gcaacgccca ctgcgtcagc gcctggtcgc
     9061 gctgcatggg cagattgttc cccaacaaaa acgtctggcg cagttaatgg tcactatcca
     9121 gaatgtcact ctggaacaga cgcaacgtaa tgtcgcactt aacgaaatgc gccagcgtta
     9181 taaagaaaag acgcagcaac ttgccgatgt gaaaaccatt tgcgagcagg aagcgcgcat
     9241 caaaacgctg gaagcccagc gtgcacagtt acaggcgggt cagccttgtc cactttgtgg
     9301 ttccaccagc catccggcgg tcgaggcgta tcaggcgctg gagcctggcg ttaatcagtc
     9361 tcgattactg gcgctggaaa acgaagttaa aaagctcggc gaagaaggtg cggcgctgcg
     9421 cgggcaactg gatgcattaa cgaagcagct tcagcgcgat gaaaacgaag cgcaaagcct
     9481 ccgacaagat gagcaagcac ttactcaaca atggcaagcc gtcacggcca gcctcaatat
     9541 caccttgcag ccacaggacg atattcaacc gtggcttgat gcacaagatg agcacgaacg
     9601 ccagctgcgg ttactcagcc aacgacatga attacaaggg cagattgccg cgcataatca
     9661 gcaaattatc cagtatcaac agcaaattga acaacgccag cagcagctct taacggcatt
     9721 ggcgggttat gcactgacat tgccacagga agatgaagaa gagagctggt tggcgacacg
     9781 tcagcaagaa gcgcagagct ggcagcaacg ccagaacgaa ttaaccgcgc tgcaaaaccg
     9841 tattcagcag ctgacgccga ttctggaaac gttgccgcaa agtgatgatc tcccgcacag
     9901 cgaagaaact gtggcgctgg ataactggcg gcaggttcat gaacaatgtc tcgcattaca
     9961 cagccagcag cagacattac agcaacagga tgttctggcg gcgcaaagtc tgcaaaaagc
    10021 ccaggcgcag tttgacaccg cgctacaggc cagcgtcttt gacgatcagc aggcgttcct
    10081 tgcggcgcta atggatgaac aaacactaac gcagctggaa cagctcaagc agaatctgga
    10141 aaaccagcgc cgtcaggcgc aaacgctggt cactcagaca gcagaaacgc tggcacagca
    10201 tcaacaacac cgacctgacg ggttggctct cactgtgacg gtggagcaga ttcagcaaga
    10261 gttagcgcaa actcaccaaa agttgcgtga aaacacgacg agtcaaggcg agattcgcca
    10321 gcagctgaag caggatgcag ataaccgtca gcaacaacaa accttaatgc agcaaattgc
    10381 tcaaatgacg cagcaggttg aggactgggg atatctgaat tcgctaatag gttccaaaga
    10441 gggcgataaa ttccgcaagt ttgcccaggg gctgacgctg gataatttag tccatctcgc
    10501 taatcagcaa cttacccggc tgcacgggcg ctatctgtta cagcgcaaag ccagcgagac
    10561 gcaggaagtc gaggttgttg atacctggca ggcagatgcg gtacgcgata cccgtaccct
    10621 ttccggcggc gaaagtttcc tcgttagtct ggcgctggcg ctggcgcttt cggatctggt
    10681 cagccataaa acacgtattg actcgctgtt ccttgatgaa ggttttggca cgctggatag
    10741 cgaaacgctg gataccgccc ttgatgcgct ggatgccctg aacgccagtg gcaaaaccat
    10801 cggtgtgatt agccacgtag aagcgatgaa agagcgtatt ccggtgcaga tcaaagtgaa
    10861 aaagatcaac ggcctaggct acagcaaact ggaaagtacg tttgcagtga aataactatt
    10921 cagcaggata atgaatacag aggggcgaat tatctcttgg ccttgctggt cgttatcctg
    10981 caagctatca ctttattggc tacggtgatt ggtagccgtt ctggtagttg tgatggtggt
    11041 atgaaaaaag tcattttatc tttggctctg ggcacgtttg gtttggggat ggccgaattt
    11101 ggcattatgg gcgtgctcac ggagctggcg cataacgtag gaatttcgat tcctgctgcc
    11161 gggcatatga tctcgtatta tgcgctggga gtggtggtcg gtgcgccaat catcgcactc
    11221 ttttccagcc gctactcact caaacatatc ttgttgtttc tggtggcgtt gtgcgtcatt
    11281 ggcaacgcca tgttcacgct ctcttcgtct tacctgatgc ttgccattgg tcgcctggtt
    11341 tccggctttc cgcatggcgc attttttggc gtcggcgcga tcgtgttatc aaaaattatc
    11401 aaacccggaa aagtcaccgc cgccgtggcg gggatggttt ccgggatgac agtcgccaat
    11461 ttgctgggca ttccgctggg aacgtattta agtcaggaat ttagctggcg ttacaccttt
    11521 ttattgatcg ctgtttttaa tattgcggtg atggcatcgg tctatttttg ggtgccggat
    11581 attcgcgacg aggcgaaagg aaagctgcgc gaacaatttc actttttacg cagcccggcc
    11641 ccgtggttaa ttttcgccgc cacgatgttt ggcaacgcag gtgtgtttgc ctggttcagc
    11701 tacgtaaagc catacatgat gtttatttcc ggtttttcgg aaacggcgat gacctttatt
    11761 atgatgttag ttgggctagg gatggtgttg ggaaatatgc taagtggcag gatttcagga
    11821 cgttattcac cactgcgcat tgcagcagtg actgacttta taattgtact ggcactgctg
    11881 atgctctttt tctgctgcgg catgaaaaca acgtcgctta tttttgcttt tatttgttgc
    11941 gcgggattat ttgccctttc agcacctctg caaatattgt tactacaaaa cgccaaaggc
    12001 ggagagttat taggtgccgc aggtgggcaa atagcgttta acctcggtag cgccgtcggc
    12061 gcatattgcg gaggtatgat gctgacgctg gggctggcat ataattacgt ggcgctgcct
    12121 gccgccctgc tttcgtttgc tgcgatgtca tcgttgctgc tgtatggtcg ctataagcgc
    12181 cagcaagcgg cggatagtcc ggtgctggcg aaaccactgg ggtagattac tgtcacggtg
    12241 cgcgttgctt attgccggat gcggcgtgaa cgccttatcc gccctacgcg gttctggcac
    12301 attttgtagg cctgataaga cgcggcaagc gtcgcatcag gcatgatgcg ccaattgcct
    12361 acgtttttta ctcttgtggc cataaccacg cagcgccgcg tacgccgctg gaatcaccgt
    12421 gcttcgcctt acgcaccggc gtttcacatt cgccgccgaa gacaaattgt ttaatcaact
    12481 gcggaaccgt ttgatataaa cggtctacat tgctcatccc gccccccagg acaatcacat
    12541 ccggatcgag aatattcacg acatgtgcca gcgattttgc cagccgcagc tcgtagcgac
    12601 gcaatgccag ttccgctacc ggatcgcttt cttcaaccag gctgataatt tcactgcctt
    12661 tcagcgcatg tccgctcaaa cgacgataat ccgtcgcgaa tccagtgccc gaaataaagg
    12721 tttcaataca accttgttta ccgcaataac aagggacttc ctcgcgataa cgcagttcgt
    12781 cttcgtccat ccacggtagc ggattgtgtc cccactcacc tgccgtgcca ttgccgccga
    12841 tatgcgcccg cccattgaat gccacgcccg cgccgcatcc cgtgccgata atcacggcaa
    12901 ataccgtctg cgctcccgct gccgcgccat ctaccgcttc tgaaaccgcc agacagttgg
    12961 cgtcatttgc cagccgcact tcccgctgca accgcgcgct taagtcttta tcgaatggct
    13021 gaccgttgag ccaggttgaa ttggcattct tcaccacacc ggtgtagggc gaaatcgaac
    13081 ctggaatgcc catacctacc gttccgcgct gccctgtcgc ctgctccgcc atatcaacca
    13141 acgtggcgat cgtttcaata gtttgccggt aatcatcacg cggcgtgggc agacgatggc
    13201 ggtacaactg ctcccctgca tcgcccagtg caatcacttc agttttggtg ccgcctaaat
    13261 cgatacctat acgcacggta ctctccttat ttttttcaat atcaatagcg tagagacgga
    13321 caaccggatt ggcaatgcaa ggccgccgac aattcgttat catgcccgct aaatttaacg
    13381 acaaggccgt ggaaattatc atgctgtggt tcaaaaattt aatggtttac cgtcttagcc
    13441 gcgagatttc gctgcgtgca gaagagatgg aaaaacagct agcctcgatg gcatttaccc
    13501 catgcggcag ccaggacatg gcgaagatgg gctgggttcc tccgatggga tcgcacagcg
    13561 atgcgttaac gcacgttgcc aatggtcaaa ttgttatctg cgcgcgcaaa gaagaaaaaa
    13621 tcctcccgtc tccggtgatt aaacaggcgc tggaagcgaa aatcgccaaa ctggaagcgg
    13681 aacaggcgcg taagctgaag aaaaccgaaa aagattcgct gaaagacgaa gtgctgcact
    13741 ctctgctgcc gcgtgctttc agccgtttta gccagacaat gatgtggatc gacacggtta
    13801 acggtttgat tatggtggac tgcgccagtg ccaaaaaagc ggaagatacg ctggcattac
    13861 tgcgtaaaag cctggggtcg ttaccggttg taccattgag catggaaaac ccgattgaac
    13921 tgacgctgac cgaatgggtt cgctccggta gtgcggcaca gggcttccag ctgcttgatg
    13981 aagccgagct gaaatcgttg ctggaagatg gcggcgtgat ccgcgcgaag aaacaagatc
    14041 tgaccagcga agagatcacc aatcacattg aagccggaaa agtggtgact aaactggcgc
    14101 tcgactggca gcagcgcatt cagtttgtga tgtgcgacga tggttcgctc aagcgtctga
    14161 agttctgcga cgagctgcgc gatcaaaacg aagatatcga ccgtgaagat ttcgcccagc
    14221 gttttgatgc cgatttcatc ctgatgactg gtgaactggc agcgttaatt caaaacctga
    14281 ttgaaggatt aggtggcgaa gcacaacgtt aattgctgat tttcctttaa tgccggatgc
    14341 gacgcctgcc gcgtcttatc cggcgtacga agccacacca ggcatataat tattcgctac
    14401 ggcgggcaat aatttttagc gcagcaatat tatgcgtttt atgctgtaac ttgctccatg
    14461 gacgttgtgt cattgttttt cctcaagcca gtgacgcgct tcactgcgaa ttttccgcat
    14521 tgccccggtt ttcactaaat cccgtaacag cgaaagatcg ggttgttcga caaataagag
    14581 taatgatgga tcctctttta accacgtcgt tttcacatcg gcagcgagct gtggattatt
    14641 catagctagc gaccgatgcc gagactcggt caatgtcgtt aacaacgacg ccggggtatg
    14701 tgcgttgcgt aatgccgttt ggcgcaaatg atcattgcga ctttgcagca atgcacgcca
    14761 ccattcatca tctgatttat cttttatcgt agccagttcc gcaacaatat ctggagatct
    14821 tcactgatat ttatatcaac cttgatgaat tatgaatata ccggaaattg aaaaaatcgc
    14881 agcaaaggaa atattattat gggttaaaga gaggatattt cagaaaaatg ccctcccgtt
    14941 caaggggaag gcgaggaatt acagatagcg gcacagataa gaggtgggtt cggcaacttg
    15001 cagatgaaac tcactgtgac cgggaacatt aaacaccgaa ccggcttcat acacctgcca
    15061 gtcggtcgcg tcaggcagta acacattcag cgcgccactg attaccgtca tctcttccgg
    15121 ctcagcggtg ctgaaggtgt attcgccttc aaccataaca cccacgctgg cgcgaccagt
    15181 gctgctgctg gaaaagccga ttgatttcac tttgccggaa aagtactcat tactttgaag
    15241 cataaactgg ccctgtctgg tagttgaaca tctacttcac tataggggcc agaggcgctg
    15301 gctgtcacgc aaaattacac cagtaattcc gcagccagcc gtgcaatcaa tacgttagac
    15361 agcaagacag gaacatcgag ctgtttttgc agtaaatcgc gatgacgttg gtgaaatcct
    15421 aaacaatcca gcatgatgac atcagcccct tttgccagta attctttccc ggcatcaatg
    15481 attttttgtt ctgaatcatg aatggggttg cccaatgaaa ataccggcga tttctgcaaa
    15541 atttgccatt tttgcgcctg aacggtcagc atctcctcaa ccggaacgat aacccccacc
    15601 tgatgatctt caacaataga ggaaaccagt ggaggcaata ttcgcgacgg ctcaagaaag
    15661 atcgtattac gcgcagtcat actactaatg tttgctgtac tcattaataa aatgacgtca
    15721 taaccctgat tatcgagcac ttcaaccaca ccttgcaggt ctcgctccac tttacgacgc
    15781 gaaacatggg ccagctggtt gtcatttaat aatgtgagaa tggtgtcttc gcctgcttct
    15841 ggcgcgtact ccgccatcac ttcttcacga cttaacttcc ccagcaagct atgatgggaa
    15901 atattatctt cgtcaatgta ttccgtcagg agcggcaaaa cctcctgcat aggtacaatg
    15961 ccgatggtca ggatcgccaa cgacgcactc attgttcatc caccttactt ttctttcgtc
    16021 gttaattacc gggcaagtgt gaagcaccac tgctgacact tacttgtgct acaaatgaca
    16081 aaaagcgtag cagcagacgt gcacggcata atgtaaagat tccaaatgat tccagtaatg
    16141 gattgttatt gtttaatatt ctaattatta gaaaaacatg aattatgaaa aatgtgacgc
    16201 agatcccttg tagtggcact cagaatccct tccgagcagt ctgatgcggt cactctttgt
    16261 cagggtcctc atagcgtttt ttcgcatcca tcgcttcctg agccgtcgga tgttcactga
    16321 tcaacgagtc tggtttagga tgatcggctc tgagttggta ccaggttacc gtctgtcctg
    16381 gctttccttt ttcaatcgtc actatatatg cttcacgagg ataaggcggt ttcgttggca
    16441 tcgttcttcc ttatttcacg ggatgaacgt taagtatagg cgctctaaaa tcaacaattg
    16501 atcgtctgtg ccagggcgct acgaatttca gaaatcacct ggctgggttc gtttgttgcg
    16561 tcgatgataa tatgcgcaac ttcgcgatat agcgcatcgc gttcttccag cacttcctga
    16621 acttcttcgc tcagcggttt tcccgttaag gttggccgta aatcttcttc cggtgcagct
    16681 tgcagtcggt taaccaggac tgatactggc gcacacaaat aaaccacgat cccgttattt
    16741 tgcatgaagt gacgattaaa ttccgtcaga ataatgccgc cgcctgtagc gataacggtg
    16801 gatggcgcag ttaccgcttc cagcgccgcc gtttctctgg cgcgaaatcc cgcccactct
    16861 tccctttcga cgatctccgc gaccgtcata ttgagctgtg attgcaacca ctgatcggta
    16921 tcgacaaacc gacggttaag cgaatcggca agggccattc cgaccgttgt tttaccacag
    16981 ccccgaggcc cgatcagaaa aagaggttgt gtcatcgtgg gttttcccca ataggtcgca
    17041 atgcggcgaa agccggtgtc atgagaatag cgatcatgcc atcaaactag tacaatttcg
    17101 attgtaaaga aaaaattcca cttaaagtga aaatctcaat acatccctta ctataccaat
    17161 aaatattcaa gaatgaagtg taaataataa attacattta gccacgacta cgttgcactt
    17221 ccagccacca cttctccagc tccgcggcaa aggcctggcg gtcacgttgt gaaaggctat
    17281 ctggtccgcc ggtctggatc ccactggcac gtaaggtatc cataaaatcg cgcatcgtca
    17341 ggcgctcacg aatggtcgct ggcgtgtaac gttcgccgcg cggattaagc gccgctgcgc
    17401 ctttctcgat ggcttcagca gccaaaggta tatctgcggt gatcaccaaa tcgcccgctt
    17461 cacactgccg gacaatttcg ttatcggcaa cgtcgaaacc tgccgcgacg cgcagcgtac
    17521 gaataaatcg cgatggcggc acgcgtaaac tctggtttgc taccagtacc agcggcatct
    17581 gcatacgttc cgccgcgcga tacaaaatct ctttaattac attgggacac gcgtcggcat
    17641 ccacccaaat tgtcataaag tcatcctttg ttgggtaatc ctctattgtg tcgcgctttt
    17701 gccttccggc atagttctgt ttatgcttct gccagcgatt atcaaaacaa tgaatttcac
    17761 ggcaggagtg aggcaatgga aaagaaaatc ggttttattg gctgcggcaa tatgggaaaa
    17821 gccattctcg gcggtctgat tgccagcggt caggtgcttc cagggcaaat ctgggtatac
    17881 accccctccc cggataaagt cgccgccctg catgaccagt tcggcatcaa cgccgcagaa
    17941 tcggcgcaag aagtggcgca aatcgccgac atcatttttg ctgccgttaa acctggcatc
    18001 atgattaaag tgcttagcga aatcacctcc agcctgaata aagactctct ggtcgtttct
    18061 attgctgcag gtgtcacgct cgaccagctt gcccgcgcgc tgggccatga ccggaaaatt
    18121 atccgcgcca tgccgaacac tcccgcgctg gttaatgccg ggatgacctc cgtaacgcca
    18181 aacgcgctgg taaccccaga agataccgct gatgtgctga atattttccg ctgctttggc
    18241 gaagcggaag taattgctga gccgatgatc cacccggtgg tcggtgtgag cggttcttcg
    18301 ccagcctacg tatttatgtt tatcgaagcg atggccgacg ccgccgtgct gggcgggatg
    18361 ccacgcgccc aggcgtataa atttgccgct caggcggtaa tgggttccgc aaaaatggtg
    18421 ctggaaacgg gagaacatcc gggggcactg aaagatatgg tctgctcacc gggaggcacc
    18481 accattgaag cggtacgcgt actggaagag aaaggcttcc gtgccgcagt gatcgaagcg
    18541 atgacgaagt gtatggaaaa atcagaaaaa ctcagcaaat cctgatgact ttcgccggac
    18601 gtcaggccgc cacttcggtg cggttacgtc cggctttctt tgctttgtaa agcgccaaat
    18661 ctgccgattt caaccactca cgatagtgac tcatttgtgg gttcagcggc gcaaccccca
    18721 cactaatccg taaagttacc tgtggcgtat tcggcaagcg taacgtgttt agcccttcac
    18781 gcacccgtaa catggcggta atggcgctct cagctggcgt accggacatg attactgcaa
    18841 actcatcgcc gccaaaccga ccaatcacat cgctaccgcg cagggtaatt tgtaactgtc
    18901 gggtaagcgc cacaatcgct tcatcgccca catcatgacc ccaggtatcg ttgatgctct
    18961 tgaaatggtc gatatcgata atcagtaacg ttgcatcgcg attatgccgc cgacagttat
    19021 caaattcatt gcgtaacata gtttcccaat gacgtcggtt atacacgccc gtcatgccgt
    19081 cgcgggtact catgacctgc aacctgcgtt tatgttccgc cagtttggtt gccgtctggt
    19141 agctgaccca gccaaacagc agaggataaa cgacaataat gggaagggag agccaccatt
    19201 ccagcggcgc actattgaac gacacggtaa tgcccgtaag ctcgagggtg acaaggcagg
    19261 aaaccaccat caacaccaga cccgcgacaa acagacgggg gccgcctgcc cccatcaaat
    19321 tcagacacat aatcatcaac atagcggtgg aaggcagcac gtttacgccc attacgccta
    19381 cccacattcc cgctaatact gcatcggttt ttaagttgta aatttcccgg ctaagcggat
    19441 cgacggccct gctcgctatc tgccaggcta aatgcggcca gacgaacgcc cagccgacca
    19501 acaccagcca ccaccagccc ggcggcgggt gcgaaaccag cgttgaagca atcggtaaga
    19561 acatgccagc caggccaacc gcacggggta gtctgacgcg acgggcgaag cgcagcccgg
    19621 accgctgatg ttcgttttga ggagttaaag gaggttcctc cccatgcgcc gccgcttttt
    19681 tgaaaaagtt ttcatcattc attatttttg ggaacattca gaaacaattt tcccaaatta
    19741 tagagacggg gctaaaggca gaagtatgaa atttcgggtg agccactttc gtagctcacc
    19801 cgttagcact atcaggtatc aggcggcttt cttgaggcag gcactcataa acttattacg
    19861 atcatcacct ttcagagatt gttgcgttgc ttgattatta cattcgcgca tcttttgctg
    19921 ctgtggcgtc aaactttttt caccaggcgc agacttgctg ttcttcaggc aatcactcat
    19981 gtaggtctta cgagcatccc ctttcaacgc ctgcgccgtc gcctgctgat tacaggatgt
    20041 catacgctgt tgttgtgggg ttaaggttct ctcggcagcg ccgacggtgg ttaaaaaaac
    20101 cagaccaaaa agcaaggtaa ccagtaatgt tattttcata gcaccatccc tcttcatgtt
    20161 ttaaccatga gcgtatgcgc ccgtgatctg ccattaagtc tggttgctaa cagcaaaaaa
    20221 accacccggc agcgaaaatt cactgccggg cgcggtttta tttcagcccc agggcggctt
    20281 tcatggtgta gaagagatcg gtctggtcgg tcagtccaac aacattggcg gcatgcgggc
    20341 catacgccgc aatacgcaac tgactgccgg tatgttcttg tgaatcctct tcggagttcc
    20401 cgtagctcat caccatcact gcgccatctt tggtatttag cgcctgggtg aggcccggag
    20461 ctttggtatc cggcgcgaca atctggctgg cgtgggcgtg atcagcggtg actatgacca
    20521 gcgtgttgcc atccttttta gcgaattcca gcgcccgttg tacggcttca tcgagatcga
    20581 ccgtctcgcc aatttgccca caaggattcg cagcgtgatc ctgtttatcg attgacgcac
    20641 cttcaacttg caggaaaaat cctttctcat ttttactcaa caattcaatg gctttgtcgg
    20701 tcatctgcgc cagggttggt acactgtcat tacgttgcgg attaggcgta caggtgactg
    20761 cgggcttatc gatattgccg tggtacgttg ctttcggtcc tagccagcgc actggcatat
    20821 tgccgtcagc aaacagtcca agcagaggtt tttgctgatt cgcttccgtc accgaattca
    20881 gtgaggcagc atcgctgacc aactgataac cacgcgcctg tgcctgttca cgcagcgttt
    20941 ttccctgcca ttcaccggcg gttgccgttt cagcaaaggt ttttgcgcca ccgccaagcg
    21001 taacgtcggc acgagcgtta agcagctgtt cggtaatcga tccttttccg cctttttcca
    21061 gagcgttacc cggacatttt tcactggtcg cgctcggacc gtagcatttg cgcgaggtca
    21121 catgcgccac cagcgcagcg ggcgtggcat cctgcaactc tgcggtagaa acgttaccgg
    21181 tcgccagacc tgcggctttt gccatttcca gaatcgttgg gtgatctttt tcgtgaatat
    21241 cgacgcccag cgcgccgtta taggttttga caccggttga ccaggcggtt gctgatgcag
    21301 ccgaatcggt gacgtagtcc ggtttgccgg tttttttatt cagcgcatag tgagtgtatt
    21361 gcccggtaag cggtaaggca tcgatacctt taaaaaagcc gcccgcacct tcggcataat
    21421 tacgtgcggc tgtaatttcc gaatccccca tcccatcgcc aatcagcaaa ataatatttt
    21481 ttgcaggttt atcgctaaga gaatcacgca gagcggcagt ctgatcaccc gttaaacggc
    21541 gagcaccgcc ggatgcagta atatcgccct gagcagcccg gttttccaga acaggcattt
    21601 ctggtgtccg ggcttttgtc acaggggtaa acagtaacga taagagtgcc agtgcaatag
    21661 tgctttgttt cact
//
LOCUS       DAZFWL020000076        21659 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFWL020000076 DAZFWL020000000
VERSION     DAZFWL020000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.076"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(42..2165)
                     /locus_tag="ACHKNP_RS14985"
                     /old_locus_tag="pgaptmp_002997"
     CDS             complement(42..2165)
                     /locus_tag="ACHKNP_RS14985"
                     /old_locus_tag="pgaptmp_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tailspike protein"
                     /protein_id="HGU4705125.1"
                     /translation="MSDITANVVVSMPSQLFTMSRSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAQQFY
                     FPNVLKYDPDQFKGNLLSEDHSLGDSLVMHNPQFVDSTSTILSKKLTIDLRTLTDYGF
                     AEGNTGAQNKAAFQKAIDDATLPTEIVIPEGAFIVDPGITIKNTVTMIRGAGAYQSRI
                     FSTGTAAPIITQQDGVITFCEFRDFGLDGNGYAANGISLTEANHIKIENIDVVNTNNN
                     AILVNGYSIDIIGCRLFQNAGNGINVGGYCNNINIINSRIYGNGAGGVLLTPAYAEGG
                     MSVRVNGNNIEQNKFYGLLAYGVKGLNLDANYWERNGEIGYPYSVPESITVRADIHLI
                     ANNFTLIPDLSKINDTVSIRGNQQTAIGYASALPNQDGFIFTNYAKNLTIENNQLLDA
                     SKVNNLLAMYHNNLSSKVTDRLYLANNTVNSIGYVGSYDPATQNPDTAHLIDIANREL
                     TANYLDRNMLLWTAASGTTGTLIKTQNIYAGNYSFLVTTGDRVWGRTIDLNKSPELKG
                     KFVWFGAWVNDQGSASKLMFIINGAGQTDSTAPLAGNGKWSYVSCGVYIYETDTAINV
                     GIRNYGSGNVLINSPSLCAYGMPSNALQVEKTTFYLSSVPTSGFWDIGERVINSAPAS
                     GQPKAWTCNIPGGPGTYSFLSEGNF"
     gene            2287..2553
                     /locus_tag="ACHKNP_RS14990"
                     /old_locus_tag="pgaptmp_002998"
     CDS             2287..2553
                     /locus_tag="ACHKNP_RS14990"
                     /old_locus_tag="pgaptmp_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU4705126.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKMVFETLKTMYSNNKKET"
     gene            2624..3109
                     /locus_tag="ACHKNP_RS14995"
                     /old_locus_tag="pgaptmp_002999"
     CDS             2624..3109
                     /locus_tag="ACHKNP_RS14995"
                     /old_locus_tag="pgaptmp_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705127.1"
                     /translation="MKKSLLIIPLLLVGCAKVSDYQASCEQRYQKLSDMANCLDASVK
                     NDSRMASAPTPKLYVLAAKMLGKGVDEGKISDAQARLELQNLYVQLQSQEQAQQIAQS
                     QAFQQALLNYQAVNTMQAIEQKARQPVITQPYPTRVDTYTNCNSGFGNTVTCNSSSNI
                     R"
     gene            complement(3124..4968)
                     /locus_tag="ACHKNP_RS15000"
                     /old_locus_tag="pgaptmp_003000"
     CDS             complement(3124..4968)
                     /locus_tag="ACHKNP_RS15000"
                     /old_locus_tag="pgaptmp_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HGU4705128.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTRDEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDKPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDSLHEVLQKTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNALLRLANTKAGTPAYERALNNAANAIRPILSSQITAEQQ"
     gene            complement(4968..6374)
                     /locus_tag="ACHKNP_RS15005"
                     /old_locus_tag="pgaptmp_003001"
     CDS             complement(4968..6374)
                     /locus_tag="ACHKNP_RS15005"
                     /old_locus_tag="pgaptmp_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HGU4705129.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIDRS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAKELTRVGVDPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            complement(6384..7076)
                     /locus_tag="ACHKNP_RS15010"
                     /old_locus_tag="pgaptmp_003002"
     CDS             complement(6384..7076)
                     /locus_tag="ACHKNP_RS15010"
                     /old_locus_tag="pgaptmp_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HGU4705130.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            complement(7079..7534)
                     /locus_tag="ACHKNP_RS15015"
                     /old_locus_tag="pgaptmp_003003"
     CDS             complement(7079..7534)
                     /locus_tag="ACHKNP_RS15015"
                     /old_locus_tag="pgaptmp_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HGU4705131.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VYGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(7534..8382)
                     /locus_tag="ACHKNP_RS15020"
                     /old_locus_tag="pgaptmp_003004"
     CDS             complement(7534..8382)
                     /locus_tag="ACHKNP_RS15020"
                     /old_locus_tag="pgaptmp_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HGU4705132.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHESRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNSATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            complement(8382..9800)
                     /locus_tag="ACHKNP_RS15025"
                     /old_locus_tag="pgaptmp_003005"
     CDS             complement(8382..9800)
                     /locus_tag="ACHKNP_RS15025"
                     /old_locus_tag="pgaptmp_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HGU4705133.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPADSGFMQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDPEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYGLQQEHLLFTPLFKADNARCF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            complement(9809..10291)
                     /locus_tag="ACHKNP_RS15030"
                     /old_locus_tag="pgaptmp_003006"
     CDS             complement(9809..10291)
                     /locus_tag="ACHKNP_RS15030"
                     /old_locus_tag="pgaptmp_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HGU4705134.1"
                     /translation="MATVLTKGEIVLFALRKFAIASNASLTDVEPQSIEDGVNDLEDM
                     MSEWMINPGDIGYAFATGDEQPLPDDESGLPRKYKHAVGYQLLLRMLSDYSLEPTPQV
                     LSNAQRSYDALMTDTLVVPSMRRRGDFPVGQGNKYDVFTSDRYYPGDLPLIDGDIPNA
                     "
     gene            complement(10266..10451)
                     /locus_tag="ACHKNP_RS15035"
                     /old_locus_tag="pgaptmp_003007"
     CDS             complement(10266..10451)
                     /locus_tag="ACHKNP_RS15035"
                     /old_locus_tag="pgaptmp_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705135.1"
                     /translation="MDRMSVFLAADNESGHVQAVIAEKDFQFFERLGFVASVDELKPT
                     SKRGRKAADNGNSTDKG"
     gene            complement(10494..11765)
                     /locus_tag="ACHKNP_RS15040"
                     /old_locus_tag="pgaptmp_003008"
     CDS             complement(10494..11765)
                     /locus_tag="ACHKNP_RS15040"
                     /old_locus_tag="pgaptmp_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HGU4705136.1"
                     /translation="MPNNLDSNVSQIVLKKFLPGFMSDLVLAKTVDRQLLAGEINSST
                     GDSVSFKRPHQFSSLRTPTGDISGQNKNNLISGKATGRVGNYITVAVEYQQLEEAIKL
                     NQLEEILAPVRQRIVTDLETELAHFMMNNGALSLGSPNTPITKWSDVAQTASFLKDLG
                     VNEGENYAVMDPWSAQRLADAQTGLHASDQLVRTAWENAQIPTNFGGIRALMSNGLAS
                     RTQGAFGGTLTVKTQPTVTYNAVKDSYQFTVTLTGATASVTGFLKAGDQVKFTNTYWL
                     QQQTKQALYNGATPISFTATVTADANSDSSGDVTVTLSGVPIYDTTNPQYNSVSRQVE
                     AGDAVSVVGTASQTMKPNLFYNKFFCGLGSIPLPKLHSIDSAVATYEGFSIRVHKYAD
                     GDANVQKMRFDLLPAYVCFNPHMGGQFFGNP"
     gene            complement(11777..12661)
                     /locus_tag="ACHKNP_RS15045"
                     /old_locus_tag="pgaptmp_003009"
     CDS             complement(11777..12661)
                     /locus_tag="ACHKNP_RS15045"
                     /old_locus_tag="pgaptmp_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HGU4705137.1"
                     /translation="MENELIIDGQVIDLSETQENAEETIIQTESQPENESQDDNGKEM
                     ATDPEKTEETPEDYALRIGDEEIQLNADDNDHIDGQPAPQWVKDLRKGFKETQKENRE
                     LRRQLEEALAKPAEHQQPQPDAIPPKPTLESCDYDEQAFEQALTDWHEKKGRVEQQQQ
                     QKLRQQQEYQQRFQQRVEAHKQRAAKLPVKDYQEMEAIVLSELPPIQQEIIIHCADEG
                     SELLAYGLGKSQQLRQRVAAETDPIRAAFLLGQISKQVSLAPKPKKAIKPEPEVRGGG
                     ADAKQDEFNKLCPGAKIE"
     gene            complement(12675..14801)
                     /locus_tag="ACHKNP_RS15050"
                     /old_locus_tag="pgaptmp_003010"
     CDS             complement(12675..14801)
                     /locus_tag="ACHKNP_RS15050"
                     /old_locus_tag="pgaptmp_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGU4705138.1"
                     /translation="MAETLEKKHERIMLRFDRAYSPQQEVREKCIEATRFARVPGGQW
                     EGATAAGTKLDEQFEKYPKFEINKVATELNRIIAEYRNNRITVKFRPGDREASEELAN
                     KLNGLFRADYEETDGGEACDNAFDDAATGGFGCFRLTSMLVNEYDLMDDRQRIAIEPI
                     YDPSRSVWFDPDAKKYDKSDALWAFCMYSLSPEKYEAEYGKKPPASLDVTSMTSWEYD
                     WFDEDVIYIAKYYEVRKESVGVISYRHPITGEIATYDSDQVEDIEDELAIAGFQEVAR
                     RSVKRRRVYVSVVDGDGFLEKPRRIPGEHIPLIPVYGKRWFIDDIERVEGHIAKAMDP
                     QRLYNLQVSMLADTAAQDPGQIPIVGMEQIRGLEKHWEARNKKRPAFLPLREVRDKSG
                     NIIAGATPAGYTQPAVMNQALAALLQQTSADIQEVTGGSQAMQQMPSNIAQETVNNLM
                     NRADMASFIYLDNMAKSLKRAGEVWLSMAREVYGSEREVRIVNEDGSDDIAVLSAQVV
                     DRQTGAVVALNDLSVGRYDVTVDVGPSYTARRDATVSVLTNVLSSMLPTDPMRPAIQG
                     IILDNIDGEGLDDFKEYNRNQLLISGIAKPRNEKEQQIVQQAQMAAQSQPNPEMVLAQ
                     AQMVAAQAEAQKATNETAQTQIKAFTAQQDAMESQANTVYKLAQARNIDDKAVMEAIR
                     LLKDVAESQQQQFQSPPQSPADLMPS"
     gene            complement(14804..16216)
                     /locus_tag="ACHKNP_RS15055"
                     /old_locus_tag="pgaptmp_003011"
     CDS             complement(14804..16216)
                     /locus_tag="ACHKNP_RS15055"
                     /old_locus_tag="pgaptmp_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HGU4705139.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHTKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDGP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPALVREETEIYVPSSSGW"
     gene            complement(16213..16638)
                     /locus_tag="ACHKNP_RS15060"
                     /old_locus_tag="pgaptmp_003012"
     CDS             complement(16213..16638)
                     /locus_tag="ACHKNP_RS15060"
                     /old_locus_tag="pgaptmp_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin carboxyl-hydrolase"
                     /protein_id="HGU4705140.1"
                     /translation="MATEKKKGGRPSDYMPEVANDICALLSSGESLRKVCERPGMPSK
                     TSVFRWLAEHQEFRDQYAKATETRADSIFEEIFEIADDVIPDAAEVAKARLRVDTRKW
                     ALARMNPRKYGDKVTNELVGKDGGAIQIETSPMSTLFGK"
     gene            complement(16718..16960)
                     /locus_tag="ACHKNP_RS15065"
                     /old_locus_tag="pgaptmp_003013"
     CDS             complement(16718..16960)
                     /locus_tag="ACHKNP_RS15065"
                     /old_locus_tag="pgaptmp_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HGU4705141.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            complement(17064..17435)
                     /locus_tag="ACHKNP_RS15070"
                     /old_locus_tag="pgaptmp_003014"
     CDS             complement(17064..17435)
                     /locus_tag="ACHKNP_RS15070"
                     /old_locus_tag="pgaptmp_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HGU4705142.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPDHIEGIIAQEAYFTAEDGAFGK
                     AIKAKHTGGEVNYQSHESLSLLTFCVLVLRNGFTVTGESACASQENFDAEIGRKIARQ
                     NAVNKIWMLEGYLLKQKLSEQ"
     gene            complement(17719..18237)
                     /locus_tag="ACHKNP_RS15075"
                     /old_locus_tag="pgaptmp_003015"
     CDS             complement(17719..18237)
                     /locus_tag="ACHKNP_RS15075"
                     /old_locus_tag="pgaptmp_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4705143.1"
                     /translation="MNHQLANLDFRDMVVVSGDRVITTSRKVAAYFDKQHHHIIQKIE
                     KLDCSDEFLTSNFSRVTYEHKGNQYVEYEISKDGAMYIIMSFTGKKAAAIKEAFIKAF
                     NWMRDRLMEMAHSYQREHNELMLEFMKEKDVASMSGRLLNRWGRIKKPQLIARIKRLE
                     QQAQISIPGLPK"
     gene            complement(18443..18880)
                     /locus_tag="ACHKNP_RS15080"
                     /old_locus_tag="pgaptmp_003016"
     CDS             complement(18443..18880)
                     /locus_tag="ACHKNP_RS15080"
                     /old_locus_tag="pgaptmp_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4705144.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            complement(18877..19353)
                     /locus_tag="ACHKNP_RS15085"
                     /old_locus_tag="pgaptmp_003017"
     CDS             complement(18877..19353)
                     /locus_tag="ACHKNP_RS15085"
                     /old_locus_tag="pgaptmp_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU4705145.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            complement(19337..19660)
                     /locus_tag="ACHKNP_RS15090"
                     /old_locus_tag="pgaptmp_003018"
     CDS             complement(19337..19660)
                     /locus_tag="ACHKNP_RS15090"
                     /old_locus_tag="pgaptmp_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HGU4705146.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            complement(20733..21356)
                     /locus_tag="ACHKNP_RS15095"
                     /old_locus_tag="pgaptmp_003019"
     CDS             complement(20733..21356)
                     /locus_tag="ACHKNP_RS15095"
                     /old_locus_tag="pgaptmp_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4705147.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(21353..>21659)
                     /locus_tag="ACHKNP_RS15100"
                     /old_locus_tag="pgaptmp_003020"
     CDS             complement(21353..>21659)
                     /locus_tag="ACHKNP_RS15100"
                     /old_locus_tag="pgaptmp_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HGU4705148.1"
                     /translation="ELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQLIWNRER
                     ISNSQDGIVKEIKGADMFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIHVQGEGA"
BASE COUNT         5186 a         5393 c         4942 g         6138 t
ORIGIN      
        1 aacataccag atcaaagtaa tcatttcaat acataggtct tttaaaagtt tccctctgat
       61 aagaaagaat aggttcctgg cccacctgga atattgcatg tccatgcctt tggttgtcct
      121 gaagcaggtg cgctattgat aacacgctca cctatatccc agaaaccgga tgtaggtact
      181 gacgaaagat aaaatgttgt tttttcaact tgcaacgcgt ttgatggcat tccatatgca
      241 catagagaag gtgagtttat gagtacattt cctgaaccat aatttcttat accaacattt
      301 atagctgtat cagtttcgta tatatacaca ccacaactaa catagctcca tttcccatta
      361 ccagctaaag gtgctgtaga gtctgtttgt cctgcaccat taattataaa cataagtttt
      421 gacgctgatc cctggtcatt gacccacgca ccaaaccata caaatttccc ttttagttct
      481 ggtgatttgt tgaggtctat cgttcgtccc caaactctat ctcctgtcgt aactaagaat
      541 gaataattac cagcatagat gttttgagtt tttatcaatg ttcctgtagt tccagatgcc
      601 gcagtccaga gcaacatatt tcgatcaaga tagttagcag ttaactctct gttcgcaata
      661 tctattaaat gcgcagtgtc agggttttgt gtcgccgggt cataactacc aacgtatccg
      721 atggagttca ctgtgttgtt ggcaagatat agcctgtcag taactttgga cgaaagatta
      781 ttgtggtaca ttgctaataa attattcact ttcgatgcgt ctaataactg attattttca
      841 atcgtcaggt ttttcgcata gttcgtgaaa ataaatccgt cttggttagg tagcgcgctg
      901 gcgtagccaa tcgcggtctg ctgattcccg cgaatagata cggtatcatt aatttttgaa
      961 aggtcaggaa ttaatgtaaa attgttcgcg ataaggtgga tatccgcacg tactgtaata
     1021 gactcaggta cactatacgg gtaaccaatc tcaccgttgc gttcccaata gttagcatca
     1081 aggttaaggc ctttaacccc gtaagccaaa agtccgtaga acttattttg ctctatattg
     1141 ttgccattaa ctctgacgct cataccgccc tcagcgtaag ctggcgtaag taaaactcca
     1201 ccggctccgt taccatagat acggctgttg attatattaa tattattgca gtaaccacca
     1261 acattaatac cgttaccggc gttttgaaac aaccggcacc caatgatgtc aatggagtaa
     1321 ccgttaacta atattgcgtt gttattagta ttaaccacgt caatattttc tattttaatg
     1381 tggttggctt cagtaagact gatgccgtta gctgcgtagc catttccgtc aagaccaaag
     1441 tccctgaact cgcagaacgt aattacccca tcttgttgcg tgattatcgg tgccgctgta
     1501 cccgtagaga aaattctgga ctgatatgcg ccagcacctc gtatcatggt gacagtattc
     1561 ttaatcgtaa taccagggtc aacaatgaac gccccctctg gtataacaat ctcagttggc
     1621 aacgtagcat catcaatagc cttctgaaat gcagctttgt tctgagcacc ggtgtttccc
     1681 tcagcaaacc cgtagtctgt aagcgtcctc aggtcgatag ttagcttttt tgagagaatg
     1741 gtcgatgtgc tatcgacaaa ctgtgggtta tgcatgacta gagagtcgcc aagtgagtga
     1801 tcttcgctca ggagattgcc cttaaactga tctggatcat actttagcac attcggaaaa
     1861 tagaactgct gcgcaccgta tgcatcataa acagccatag aatggccttg cacagttacg
     1921 aacttggcaa tctgtccgtt atatacaggg taaccagcag cgttaatgat gattggttgc
     1981 gaaacaggaa cgtgagaacc gtcttcgttt tccacataaa cctgaatctg gttttctgga
     2041 tttaccgggt cagtgtcaat tttaccgata taaatttttc cattggctac ggctttaaaa
     2101 gagcgagaca tagtgaagag ttgcgaaggc atgctgacca caacatttgc ggtgatatct
     2161 gacatttcat tgctccagaa gaatgatatg atgcaaccat gatgtgattg cataccgaaa
     2221 tggtactatt gagtatttat ccagtaggtt acgatgccat tccacccaac tggtgaggca
     2281 tcaaggatgt acagcaaata cgacgaggcg cagtttcact tgagacttcc gcatgaactc
     2341 cacgcgaaaa ttaaacagcg cgcgaagatg aataacaggt cgctgaactc agagataatt
     2401 gcagcgattg aagaatcgtt ggctaaacaa agctctgcat ccgtttacat tgatgatgca
     2461 gagcgtatgg cagaacaaca atctgatatg gttaagaaaa tggtttttga aacgcttaag
     2521 accatgtata gcaataataa aaaggaaaca tagaaatcta gtttccggct aaaatggcat
     2581 tgccttcatg atatcctgtg aaaaactaag gagagttaac catatgaaaa aatcactgtt
     2641 aattatcccg cttctgctgg ttggatgcgc aaaagtaagt gactatcaag caagttgcga
     2701 acaacgctat caaaagctta gcgatatggc taattgcctt gatgccagtg tgaagaacga
     2761 ctcacgcatg gcatcagcac caacacctaa gctgtatgtc cttgctgcaa agatgctcgg
     2821 gaaaggtgtc gatgaaggca agataagtga cgcacaggca agacttgagc ttcagaatct
     2881 ttatgttcaa ttacaaagcc aagaacaagc ccaacaaata gcacaaagcc aagcattcca
     2941 gcaggcttta ttgaattatc aggctgtaaa cacaatgcaa gcgatcgagc aaaaagcgcg
     3001 ccagcctgtt ataactcaac cttacccaac acgcgttgac acatatacaa actgcaattc
     3061 aggatttgga aacacggtaa catgcaacag tagcagtaat atcaggtagt tacatcttat
     3121 cttttattgc tgttctgctg taatttggct tgagagtata gggcgtatcg catttgcagc
     3181 attatttagc gctctttcat aggctggtgt tcctgctttg gtgtttgcca aacgtaagag
     3241 agcattcctt gctgctttgg actcatacaa gcgcatcatt gcaccgaaac cagcctcaag
     3301 ccccattgat acgccaagag tcgcagttgc gccaatcgtc cttatcctgt tggcttgcga
     3361 ttgcccagtc tgagttacta catttgcggt gtctgacctt gctgttttct gtagaacttc
     3421 atgaagagaa tcaagctctt tcatgtgctt tccagaaaaa atagtgttgt aaatttcacc
     3481 acctgactga gatttcagct tattaacttc agtgatgaac ttggctggag agtcaccggc
     3541 cttttccgct attttgctga cgtaagctgc acgcatagca tctttccctt tatcatccaa
     3601 tgcgctccag attcgtttca cgtcagatgg ttttctgctt aatacaacag tatttataag
     3661 ttcaggactg gcttcactgc ttgccttgtt gagcttgtta gcaatgtttt tattaagcac
     3721 cttattataa acgtttgcat aatcggaatt tgctttaagg tattttgctg cgtctgacgc
     3781 accgaggttt tttgcaactg cgttacgaag gtcttttgac attgcattct ctaccatatt
     3841 ggtagctgct tttgcctggt tggggaagac catagcatct ccctgaacat tagatctaaa
     3901 tgctgttctg tgctgacgca agagatcaaa cgtaacatcc aaatcagttg cagggtttgc
     3961 taattcttca cgcaggttac gcaaggatgt aagcaggctt tgattggctg aagtcccaag
     4021 ccgttcctgt cttgcgatcg ctgtattcag agcattcatg gtatttgtag tatcaactgc
     4081 ggcattaccc attttattgg tgacgtcatt gataacagcg ccagcagcat ccttccgccc
     4141 ccttaacgtg gtggtcagag atttcaccac atcatcaggg ttgtactcac caaaacggtc
     4201 aaaataattg cttaccagct tactacgcgt tgcatattgc tctgctcgct ttgagcccgt
     4261 cccgagcaaa gccccctcgg catcctgagt aaggccgcga gtgaaagcat ttttcggcgg
     4321 gataacatca gatgtcattg gtgtcacgcc catcgattct gatgtggcaa ttttcttcgc
     4381 cacttctggc gcaatatcac cttttatagc cgttattcca cgccctattc cctttgctgc
     4441 tgcggaaaga acaccctgag cggcaaggtt aactccggca tttttagctg cattttgtgc
     4501 gaaatcgcct ttctgatttg cggcctctgc cagcgatcca atagccatgc ttcctgccgt
     4561 tccaactcct ggaactaaat acccaccaat tgtttcaccg gcttgtgcgt atgggtctgt
     4621 cggtttgtct actggacgat aaacatcatc caaaactttt ggcccaccaa gcccctgact
     4681 gattgcatta atcagacttg cgccaccctg caatacgtca aatggtatgt ttaccagacc
     4741 acgaccagcc tgttctgcaa tttgccctgc actttgacca ccagtgagcc aatcgccagc
     4801 ttgttgcatc aatgatggtt cgtcccgtgt tggtgcatta ttggcctgat taactgtttg
     4861 ttgctgaaca gcctgaccag caaaatactc atcaatggcg gtgccaatat cttcggtgct
     4921 cgtaccatca ggaaaggtaa atgtcttacc gtttgcagtt actttcatca ttccaccgta
     4981 aattgaatgc ctgattttga ggtatatgat ccaacctgat tccgtggttc tcctgaaggt
     5041 gtcgaatctt gtgctggcgc tgcgtcagta ttcattgaca tataccgctt aacggcactc
     5101 cccaatgatt cacctttttt aacatccaac cccaatatct gaccgccatt acgcgattgt
     5161 ccagggttgc cattcgcgct catccactcg gctttaaact cattaaactg cgcgtttcgt
     5221 cgctcaaggt ttgccattgc atcaagccat cttgcgaccg tctcagggtt atccatgtca
     5281 gttggcgcac cctgtcgaac gatctcaacg tctttatccg ttgctgggcc gggaggtagg
     5341 aatttaagaa cctgactgtt aacaagggca ttttggcgaa tgcgcaaatc acgcaatgtt
     5401 gtatcgcttc cggtaagttt tgcgaacatg ttctgtgcgt taccgaacaa acctgtcgtt
     5461 ggtttttctg ctctgaactg ttgagcaagc gcactcatag aattggctga gtttgatgat
     5521 gctgtagcat tgtttacagc cgtctcgatg cctttttcca tgtttactga cagcttaggt
     5581 gcttcgctaa tcaactgctg agccttttcc tgcgcttgct gcatcttaaa cccgaactct
     5641 tgctgatcca gagccaagcg ttgtgctgcg atattgtgcc cagtcattgc tgactgatag
     5701 gaaaggtttt gccctctcgc ctgaagtgct tctccagcct gattgctgcg gattgtctct
     5761 gccagtctgc ctcggtcaat ttcacgacca gccatcttgt cctgaacatt gaagtaatca
     5821 attggaccaa gagcagccat tccaaggtga tcaacaaact caccaaatcc tgaagggttc
     5881 tgctgataca tctgagcaac gctgttaggg tcaacaccga cgcgagtcag ttccttggcg
     5941 ttgttttgca gccatgattg cattgcttct ggagacgatg acgcaaggcg tgcgccagcc
     6001 gctaaggtgc cgatagaatt acgctggtct tcatcaatga atcccatgcc tttacgaacg
     6061 gattcaatct ggtctggata ttgagtagcc aactgacgca aagcaccgcg atcaccagac
     6121 gcataagcat tagcgtacgc ctgctgaaat tctttctgcc gctgagcctg cttttcctgc
     6181 tgaaacaccc ctgcaatacc tgaaaggcct tgcaaagcag tcagcccaac attgttagcg
     6241 cctgaacgat caatatcatt gttctgcctg ataagctgaa gcgtattgcc gatgtcattt
     6301 acgctcggag cgtttgagtt gacgccgccg ataccagcca acaatccgcc gtttgttcct
     6361 tgccaagtag ccatgattac cccttaaaac aacgagccaa gcaatccgat accagcacca
     6421 atgccagcgc cccaaggcgt tgatgttccc aaaaggctgg caagacctgc accggcaatc
     6481 gcaccagatg tgccgccgct aattgcagtc tgaagacttg atggtttatt ggcattagca
     6541 gcggcaagtg ctgcgctttg ctgtgcaatg ctgctcatgt tgttggcgta cgtctgcccg
     6601 gcgtttgcct gaccttgcag tgcaccaagc ccaacgtttg ccagattgtt gtaattgctc
     6661 atctgatttg ataaccaaga ctgaccgagt gtcggcgcga tcgtagccag ttgattgctt
     6721 gtggctgtcg aaccaagtcc acccgtcgcc tccgcagcag caagactctg gtaacgagcc
     6781 tgacctgcaa ggtctttata ctgctgagag ttgtaatact gattaagtgc ctgcccctga
     6841 ccttctaaac tggaaagatt ctgaagctgg ttaacatact gctccgcaag cggcgtgaac
     6901 ggagcaaggt ttttcatgat cgtctgccac tgctgatttt gcaggtctgc ggcatacttc
     6961 tgagcttctg ctgcatactt tgcgctttta tcagagctgc cacctttccc gcctttttca
     7021 gggcaataag gttcctcgcc gcgcagtttt ctgcccagct taaatgcata taacatagct
     7081 atctcccgtg attcaggaag tcgattagtt cttcgcgtgt ggcgctgtaa aacgtcacgt
     7141 catccacgcc tttgaagtat ttcttgatgg ttcctacacg cttaaggcca atcattgcgc
     7201 agtacatctg accgtggcgg aatttgcgcg cagcgaacga tgtgacgcac tgaacggtgg
     7261 tgttagtcag aatgtatcgc cagaacgcca gcccgatttc cttgctgaat ccacgaacct
     7321 ctggcaggta catggcgtgg caatcgaatg taagcggctg aatttcctga tagtaaacaa
     7381 tgccgccaaa ctgaccgtac acgttaacct caaagtaacg gcattcaggc ttgtagtcgt
     7441 atccatcacc gttgttgctt ccggcaataa tgtcagggtg atttccgact gcttctatca
     7501 ggtcgatgtt tcgcgttggt ttgaactgaa tcattactgc tccgcgatta tcttgatggt
     7561 tgtggcagta aacgccgcac catttgactg aatggttaac gtgctgccat ttgtggcaag
     7621 aaagccgtct ttatccacgc tgaagaacgt agctaacagg atgttatcgg tcgttgtcgc
     7681 cgagttgcga ctgcttacca gtgtgtcagg aacagagccg gaaaatgtta gttgcattga
     7741 cctgttggcg gttccgctgg gccacgtccc gacgatcgac agcttgaaga acaaggtttt
     7801 gttctcgttg aacacaacca tcttgttgtt aacggtgtcg aagaatggtg ccaacgagcc
     7861 tgatgacggc gtgagcgttt tcagcaggct aacaaggttg gtcggcgctg tcgggatggt
     7921 tacagatacg ccagagtaaa caacctctga cttcttgcga gtagtggcat actccagagc
     7981 atcaatgcgc gattcatggt ctgaaacctg cgattccagc gactgaactc tggtatcaag
     8041 cgacgcaata tcgctttcat tctgagcgat tcgcgtttca tgttcctgaa gagttgattc
     8101 tgcctggctg attcgctcct catgattaac aagcgttgct tccgcagcag aaattcgctg
     8161 ctcatggtca gcgagaatca catcctgctc atcgttcctg acttgtgcgt cataagcgcc
     8221 ctgtccggcc tcgttggcct tgttagccac attaccaaca tcagtaccct gtgcgataac
     8281 gtaaagcaga tacgactgcg agaagatatt gcgtggaagg actgatgtgt cgagccgtgt
     8341 agcctgaatg attaccggca cattgagatt cgaatccgcc attactcaat ccttatctga
     8401 gcgccagaca gagtgacagg cgacttagtg ataacgcgca gtttgaagcc aacatttttc
     8461 ctgatgcgcc ctactcgctt ccacaaaacg cgtttgtcgt aaacgaacgg ttcattctgc
     8521 tcaatcatct gctcacgccc gtaatttatg ccgtcagtgg ttgcagagag gaacaggcgg
     8581 tcagcgtact gagcaacacc cgtcgatgat tccacctcca gatcaaagca tctggcgtta
     8641 tccgctttga acaacggagt aaacagcagg tgttcttgct gtagcccata ctggctgctg
     8701 atatcgaact gcaatttccc ggtaaccgat tccagcttat cgccgcacgt tatctgattt
     8761 ccttcgtaaa tgaagtcgat agcgcggtac acatcgtcat acaggcctgt tttcaacaca
     8821 caccattgcg gaccattggc gcttgaagat gcgtcgtaca cgaggacgtg gcgcggaagg
     8881 tggataatca gcagttcatg cgcatcaaat cgcagcgatt ccatcacacc atcagccagt
     8941 tcatcagcag tgtaggagcg aagaattttc tcaatgctcg cgctggcgat tggtgatacc
     9001 tgaccggagc cgatgatata cacagacggc gcacctgttg ccggattgct gatgaacgca
     9061 tacgaatcgg cgaacggcgt tttgcagtaa gtcccggcaa tgcctttctg caccatcagc
     9121 gatggctgtg cgacatacaa agcggcacca acggtggttg cccccgtcag ggagaaatat
     9181 tcaatcgtcg atgaaccaaa gcagacgatg aagtctcgcc atgttccgat gccgatgatg
     9241 ccgtcaggct gagactcggc tcgatattgt gcgctgtagc ggtcaggatg cgattcgtct
     9301 tcagggtcag tgataaacca tgaatcagtg ccgtcttttg accacgcata acgcccacgt
     9361 aaacgcgtaa tgtcgcgaac agaacctaac tcatactgca taaacccgct gtctgcaggc
     9421 cagtttgaga cggttttaac cgtgccatca tagcggtatt cgaccagttg cccgttaacg
     9481 cctaccgcct gtgatgttcg accatgcgcc attgatacgc gaccacttcc ggcaacatca
     9541 ccgacctcac tttcgccctt atacagtttg ccaccacaca cgcgataaac agcattctgt
     9601 gccatgttgt actcgacgcc tcgcgatacg ccgttcacat cagaacgttt ggcaatgccc
     9661 gggaatgagc gaagatatcc gctgctgttc aggatttctt tgggtgtagc caacatattc
     9721 actggcagat agtcgatata gtcggcgttt ctaaagtctt tgccgacacc tttcataagc
     9781 ggaagttgct gaataggcat ttattcacct atgcgtttgg gatatcgcca tcaatcagag
     9841 ggagatcgcc tggataatat cggtcagatg tgaacacgtc atatttatta ccctgcccta
     9901 caggaaaatc tccacgtcgt cgcattgaag gaacaaccag agtgtcggtc atcaaggcat
     9961 catatgagcg ttgggcgtta ctgagaactt gcggagttgg ttcaaggctg taatcagata
    10021 gcattctcag caataactga tagcctactg cgtgtttgta ttttcttgga agacctgact
    10081 catcatctgg taatggctgc tcatctccag ttgcgaaagc gtaaccaatg tcgccggggt
    10141 taatcatcca ctcggacatc atatcttcca gatcatttac accatcttca attgattgcg
    10201 gctcaacatc agtaagcgat gcattagaag caatagcaaa cttacgaagc gcaaaaagga
    10261 cgatctcacc ctttgtcagt actgttgcca ttgtccgccg ccttacgacc tcgcttactg
    10321 gtcggtttca attcatcaac tgaggcaaca aagcccaacc tttcgaaaaa ctggaagtct
    10381 ttttctgcga taacggcctg tacatgcccg gattcgttat ctgcggcaag gaatacactc
    10441 atccgatcca tattgtttcc ttaaaacata aaaggggcgt aagccccttg ttattacgga
    10501 ttaccgaaga actgaccgcc catgtgtggg ttaaagcaca catatgcagg cagtaagtcg
    10561 aagcgcattt tttgcacgtt ggcatcgcca tctgcgtatt tatgtacgcg gatggagaaa
    10621 ccttcatatg ttgcaacagc agaatcaata ctgtgcagtt tcggcagtgg gatagatccg
    10681 agtccacaga agaacttgtt atagaacagg tttggcttca ttgtctggct agcagtgcct
    10741 actacagata cggcatcgcc tgcctctacc tgacgactta cagagttgta ctgcgggttt
    10801 gtagtgtcat aaatcggaac accagaaagc gtaaccgtca catcgccact gctgtctgaa
    10861 ttagcatcag cagtaaccgt tgcagtgaag ctaattggtg tggctccgtt atacaacgcc
    10921 tgtttggtct gctgttgcag ccagtaggta ttggtgaatt tgacctgatc accagctttc
    10981 agaaaacctg taacgctggc tgtcgctccg gtcaatgtta cagtgaactg gtatgagtct
    11041 ttaactgcgt tataggtaac agttggctgt gttttgactg tcagtgttcc gccaaatgcc
    11101 ccctgcgtac gagaggcaag cccattagac atcagtgcgc gaatgccgcc aaaattggtt
    11161 gggatctgtg cgttctccca tgcagtacga accaattgat ctgaagcatg caaaccagtc
    11221 tgcgcatcag caagtcgctg tgcagaccat ggatccatta cagcatagtt ttcaccttca
    11281 ttaacgccga ggtctttcag gaaagatgcc gtctgcgcaa catcagacca tttggtgatt
    11341 ggagtattgg ggctaccaag tgacaacgca ccgttattca tcatgaagtg agcaagctct
    11401 gtttcaaggt cggtaacgat tcgctggcga accggcgcga gaatttcttc cagctggtta
    11461 agcttgatcg cttcctccag ttgctgatat tcaacagcaa cagtgatgta gttacctaca
    11521 cgccccgtag ctttacctga gatcaggttg tttttatttt gccctgaaat atcaccagtg
    11581 ggagtacgga gggatgagaa ttgatgcgga cgtttaaagc taacgctatc gccagtgctg
    11641 gagttgattt cacctgccag caactgacgg tctacggttt tcgccagaac taaatctgac
    11701 ataaaacccg gaaggaattt tttcagaacg atttgactga cgttactgtc gagattgtta
    11761 ggcatttatc ttttccttat tcgatttttg cgccggggca taatttgttg aattcgtctt
    11821 gtttcgcatc agcaccgcca ccacgtactt ccggctctgg cttgatggct ttctttggtt
    11881 ttggagcaag gcttacctgt ttgctaatct gccccaagag gaatgctgcg cgaattggat
    11941 ctgtctcagc ggctacacgc tggcgtaatt gctggctctt acctaagcca taggcgagta
    12001 gttcagagcc ttcgtctgca cagtgaatga tgatttcctg ctgaattggt ggtagctcac
    12061 taagaacaat ggcttccatt tcctgataat ctttcacagg aagtttggct gcccgttgtt
    12121 tatgcgcttc taccctttgc tggaaacgct gctggtattc ctgttgctga cgtagttttt
    12181 gttgctgctg ctgttcgaca cggccttttt tctcatgcca atcagtcaat gcctgttcaa
    12241 acgcctgttc gtcataatca cacgactcaa gagtcggttt tggtggaata gcgtctggtt
    12301 gtggttgctg atgttccgca ggcttggcta atgcttcctc aagctggcgt cgcaactcac
    12361 ggttttcttt ctgtgtttct ttgaagcctt tgcgaagatc tttcacccat tgcggtgcag
    12421 gttgcccgtc aatgtgatca ttatcgtcag cgttaagctg aatttcttca tcaccaatac
    12481 gcaaggcgta atcttctggt gtctcttcgg ttttttcagg atcagttgcc atctcttttc
    12541 cgttgtcatc ctggctttca ttctcaggct gtgactctgt ttggatgatg gtttcttctg
    12601 cattttcctg tgtttcagac aggtcaataa cctgaccgtc gatgatcagt tcgttttcca
    12661 ttgattactc ctggttaact cggcattaag tctgccggtg actgtggtgg tgactggaat
    12721 tgctgttgtt gtgactcggc gacatctttc agaaggcgta ttgcctccat cactgctttg
    12781 tcatcgatgt ttctggcttg agccagttta tagacagtgt ttgcctgact ctccatcgca
    12841 tcctgctggg cagtaaatgc tttgatttga gtttgagcag tttcgttagt tgctttttgc
    12901 gcttctgcct gcgctgctac catttgcgcc tgagcgagaa ccatttcagg atttggctgg
    12961 ctttgtgctg ccatttgcgc ctgttgaaca atctgctgct ctttctcatt gcgtggtttt
    13021 gcaataccag atatcagcag ttggtttcgg ttgtactctt tgaagtcatc aaggccttcg
    13081 ccatcgatat tgtccagaat aataccctga attgccgggc gcattgggtc tgttggaagc
    13141 atagagctaa ggacatttgt cagtacagaa accgttgcat cacgtcgtgc tgtgtagctt
    13201 ggtccaacat caaccgtcac atcgtatcga ccgacagaaa ggtcatttaa cgcaacaaca
    13261 gcccctgttt gcctgtcaac aacctgtgcg ctcaggaccg cgatatcatc acttccatct
    13321 tcgttaacga tgcgcacttc acgctctgaa ccgtacactt cacgcgccat tgacagccat
    13381 acttcaccag cgcgtttaag acttttcgcc atattgtcca gatagataaa cgaagccata
    13441 tctgctctgt tcatcaagtt gttaaccgtt tcctgagcaa tattacttgg catctgctgc
    13501 atggcctgac tgccgcctgt aacctcctga atatcagcac tggtttgctg tagtaatgca
    13561 gccaatgcct gattcataac cgcaggctgt gtatatcctg ccggggtagc tccagcgata
    13621 atgttgccag atttatctct cacttcgcgc aacggcaaga acgctgggcg tttcttgttg
    13681 cgagcctccc agtgcttctc aagtccacga atttgctcca tgccaactat agggatctga
    13741 ccggggtctt gcgctgcagt atcagccagc attgatacct gaaggttata caaacgctgt
    13801 ggatccattg cttttgcaat atgtccttcg acacgctcaa tgtcatcaat gaaccagcgt
    13861 tttccataaa ccgggatgag ggggatatgc tcaccaggaa tacgtcgagg tttctcaagg
    13921 aaaccatcac catccactac ggatacatac acacgacggc gcttcactga gcgccttgcc
    13981 acttcctgaa atccagctat tgccagttca tcttcaatat cttcaacctg atcactgtcg
    14041 tatgttgcaa tctctccagt gattggatgt cgataactga tgacgccaac agactcttta
    14101 cgaacttcgt aatacttcgc tatgtaaata acatcttcat caaaccagtc atattcccaa
    14161 ctggtcatag acgttacatc cagagaagca ggaggtttct ttccgtattc agcctcatat
    14221 ttttcaggtg acaacgaata catgcagaac gcccacaacg cgtcagattt gtcgtacttc
    14281 ttagcgtcag ggtcaaacca cacagagcgc gacgggtcgt atattggttc aatagcaata
    14341 cgctgacgat cgtccatgag gtcgtattca ttgaccagca tcgacgtcaa acggaagcaa
    14401 ccgaaaccac cagtagcagc gtcgtcaaat gcattatcgc aagcctcacc gccatcggtt
    14461 tcttcgtagt cagcacggaa cagaccattt aatttattgg ctaactcttc gcttgcctct
    14521 ctgtcaccag gacgaaactt aacagtgatt ctgttattgc ggtattctgc aatgatgcgg
    14581 ttaagttcag ttgctacctt attgatttca aacttaggat acttctcgaa ctgctcatca
    14641 agcttagttc cagccgccgt tgctccttcc cattgacctc ctgggacacg agcaaacctc
    14701 gtagcttcaa tgcacttttc gcgcacttcc tgctgtggag aataggcgcg gtcaaacctg
    14761 agcatgatcc gctcatgttt tttctctaat gtctctgcca tgtttaccaa ccggaggatg
    14821 agggaacgta tatttctgtt tcttcgcgga ccaatgccgg gcaatgcata cacatcatca
    14881 gcgcatcagc caggttagga gatgggatac cgagcttctg cttcatttcg accttagtca
    14941 taagctccag cttcccgtta ttattgaatt tgcgctgaat ctgcgtcagt tctgcaaaca
    15001 gcttctccag catcttctcg cctatcgctt ctttgtcgaa gctcagcatg tcgtcggggt
    15061 ctgcatactc accgtggaca accgcccgat atgtcagata cagcctgtca gccagcgcgt
    15121 aatagaattg cgctcgctta ttgcggaata catcgccaat agtgcgaacg ttgtcgccct
    15181 gtacgacttc atcagcccat gctccggcct gataaggccc atcttcatcg aatggcgatt
    15241 cgctgccctt gaacatcgtg gcggtaattt tcttgccgga gaatgcttcc gttgtctgtc
    15301 tgcgtaggcc cgcaccgacg ccatcaccgt cccacaggta atggtcagcg ccgtcttcaa
    15361 tcgccagcga agtagcccag tcagcaccct cgttgatgtc catcagcaga ccttcggcaa
    15421 tgcgcttaac taccgaaccg tgacgcgatg cataaccttt agcatctggc cctgtatctg
    15481 atgggtcatg cgcagaaaca accgcacctt tcgctttcca cccgagtttc gtgtgcgcat
    15541 cggttgcggc ttcaagccat tcacgtttga tgattgccat atcacttgcg cttaccggct
    15601 caccaagcca gatgtgacga tacagtgtcg gatttctgcg tttacactct tccatctcca
    15661 gacggaggac ttcaggaaaa tgcgggttgt cggtgtagtt caccgtcagc aggcaaatat
    15721 catcgggagg atttacgacg aatcgctgat aggtatcgtc gaggatgttt ttcgggttga
    15781 aacttaccca tatttcagag aacggcttac ggatggttgg aatcaggata tcccacgatt
    15841 ccttcgttac cgcttccgct tcttccaccc agcagatatc aatgccttcg agcgatttaa
    15901 tcttcgtcgg gttgtttttg atgccgtaga acatgaattc agcattcgtt ccgagatgac
    15961 gaatcattga acgctgaatt tcaaactcag ccgaataccc ttcacgctcg atggtgtctt
    16021 caagcaacct gattaccgaa tcgctgatac tgttttgcag ctcacgagcg caaagaatac
    16081 gcacaggctg ccgacgcgcc gcttcaacaa gaagcctcgc aattgcccat gacttaccgc
    16141 tacctcgacc gcctttggcg actttgtagc gatgagcctc aatgaacggt tcaaagatag
    16201 gattaatcga ggtcattttc cgaatagagt gctcatcggt gatgtttcaa tctggattgc
    16261 gccgccgtct ttgccgacaa gctcgttagt taccttgtcg ccatacttac ggggattcat
    16321 tcgggccagc gcccatttgc gggtatcaac gcgaagtctt gcctttgcca cctcggcggc
    16381 atcagggatt acgtcgtcag caatttcgaa tatctcttcg aaaatagagt cggcccgagt
    16441 ctctgttgcc ttcgcgtact ggtcacgaaa ctcctgatgt tcagccagcc agcgaaaaac
    16501 tgatgttttg ctcggcattc ctgggcgttc gcaaactttg cgcagactct caccggagga
    16561 aagcaatgcg caaatgtcat tagccacctc cggcatataa tcagaggggc gaccaccttt
    16621 ctttttctca gtcgccatat tgattatttc ccttctgctt gcttatccca ttcatcgcgg
    16681 aatttggatg ggttgtcgaa accttgagtt gccatgttta cgctccggta gtgaacaggt
    16741 ctaacgcttc cttcgattta cgcaccgctt cgattgtgcg ggtcgtgata tctgaattag
    16801 cgccacctga ctggaagtga attttgaata gctcaagctt cagctcgtca gtgccaatga
    16861 actgaaatgc ttcctccgcg gctgcgttct ggttcatgac cagtttgtaa atctctaact
    16921 ggaatttctg ttcttcagtc atgggaataa tctctgccat tgttggctcc gtttatccgt
    16981 taaaagggat atcagttaag ttatcccgtg tagggtataa gccattatca aagccactca
    17041 gtaaggaatg gcttttgtaa taactactgt tcgcttagct tctgcttcag caagtaacct
    17101 tcgagcatcc agattttgtt tacagcattc tgccgggcaa tcttccgacc aatttctgca
    17161 tcaaaatttt cctgacttgc acaggcactc tctccggtga cggtgaagcc gttgcgcagc
    17221 accaggacgc agaacgtcag cagagaaagt gattcgtgcg actggtagtt tacctctccg
    17281 ccagtatgtt tcgcttttat ggctttgcca aaggcaccat cctctgctgt gaaatatgcc
    17341 tcctgagcaa taattccttc gatatggtct ggcgtaactc gcggtgccgt tttgcctttc
    17401 tcaacgattt ctttttcgat ttgctggtcg ttcataatta tgaccctgta gagtggttgc
    17461 ttgattagga tgtctttcca tcagttcgcc accacaaaga atcttttttg ccataaggct
    17521 ggaggttcat ctttcagtgg ctgccagtgt tatttcccca cttactggct tgggttgttt
    17581 cgcggtactg ccgtaactgg tggtgcacag atttagttaa atctgttctc gcctgaacta
    17641 tcttttacat acccggattg tggggatgta aatcacggtt tcattatcaa gcccacccgt
    17701 agatgggctt tggaatggtc actttggcag tccggggatc gatatttgcg cctgctgctc
    17761 aagccttttg attcttgcta tgagttgcgg ttttttgatc ctgccccagc ggttcagcaa
    17821 gcgtcctgac atactggcaa catccttttc cttcatgaac tccagcatta actcgttgtg
    17881 ctctctttgg tatgagtgag ccatctccat cagcctgtca cgcatccaat taaatgcttt
    17941 gataaacgcc tctttgatgg cggcagcttt tttgccggta aacgacatga tgatgtacat
    18001 cgcaccgtct ttggaaattt catattcaac atactgatta cccttgtgtt cataggtaac
    18061 ccgcgaaaag ttgctggtta gaaattcatc cgaacagtct agcttttcga ttttctgaat
    18121 gatgtggtga tgctgcttgt cgaagtaagc tgctaccttg cgggaggttg tgatcacgcg
    18181 atcaccagaa acaaccacca tgtcccggaa atcgagatta gccaattgat gattcatagc
    18241 gtctttacct tttagaaagt gagcctgtct cacagaaaag ccgccccgag atggtcgcca
    18301 ccatatacgg cagttctcag gctcaacttt ctgaaaggct cgggtgatgt aatatgcgcg
    18361 tgagatgcgc tgtgaaattc agatgtaaaa aaagccccgc atcgcgaggc tcattaaatg
    18421 gactttgtga tttgcaaaaa aattatttca ggcactgagt cctgatgtac tcctgcaggt
    18481 agttaacctg cgcggttatc ctgtcgattc cacttcggag acggtaataa ttgagttcag
    18541 catctgctgt aagtcttggg ctttctccat cgcccatgct gctggctccg gtcgttgact
    18601 ttgcacaggt ggcggcgact tgcaggcgct tacgaccagc agaaacatca gcacgaaggc
    18661 tttcgatagt agcgttagca tcagcaagct cctttgtgta tctggcgtcg agttctgcta
    18721 catcacgttg acgcttctgc atatcagcga tgatggatgt ggctttatcg cgctgctctt
    18781 tgtaggtcat ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc
    18841 agataaccag agcggagata atcgcggtga ctctgctcat acctcaatct ctctgaccgt
    18901 tccgcccgct tctttgaatt ttgcaatcag gctgtcagcc ttatgctcga actgaccata
    18961 accagcgccc ggcagtgaag cccagatatt gctgcaacgg tcgattgcct gacgaatatc
    19021 accgcgatca atcatcggca aagcgccacg ctctttaatc tgctgcagcg caacagcgtc
    19081 ctggcttttg ggagagaagt ctttcaggcc aagctgctta cgataggcat cccaccaacg
    19141 ggaaagaagc tggtaacgtc cggcggctgt tgatttgagt tttgggttta gcgtgacaag
    19201 tttgcgagga tgatcggagt aatcagtgaa tagctctccg ccaacaatga cgtcataacc
    19261 atgatttctg gttttctgcc gtccattatc tgttccctct gaccacgcca gcatatcgag
    19321 gaacgccttg cgttgattat tgatttccac catcttctac tccggctttt ttagcagcga
    19381 agcgtttgat aagcgaacca atcgagtcag taccgatgta gccgatgaac acgctcgtta
    19441 tataagcgag attgctactt agtccggcga agtcgagaag gtcacgaatg aaccaggcga
    19501 taatggcgca catcgttgcg tcgattactg tttttgtaaa cgcaccgcca ttatatctgc
    19561 cgcgaaggta cgccattgca aacgcaagga ttgccccgat gccttgttcc tttgccgcga
    19621 gaatggcggc taacaggtca tgtttttctg gcatcttcat gtcttacccc cagaagggga
    19681 tctgttcaaa ttaggaatta tggatatggt cgcttgaaca aatccgggtt acggttgatt
    19741 tgtaacgggt ttgttcgtga ccgcattcat gagcaaatca ggcgtggatt gcgccaacaa
    19801 tacatgccgc tcatatcacg aagcccagcc attgatgctg ggttttcttt tttaaagcgt
    19861 actagacaac cgtatccaca gagtgtcagc aatgagttgg ttaggtctgg ttcttggtgg
    19921 aagtacgctt taaaaaatgg gctgatggtg tagcccaaaa tactgagtga atggtaagga
    19981 tgaacaacgg ttttgctctg ggtggatttg gctgtggtgg ccggcgctga tctccggctt
    20041 gtatacaggc accttgttct tccgaagctc tcctgcgcgc atcagcctgc gcattcacca
    20101 caccggaaag agcactcagt tgtaccggcc agttgtgcca ctaagaaatg ctttcgcaga
    20161 ccgttaagct ctttgccagt tctttaatgc tcttacctgt tgtgtgccca ttattaatca
    20221 caccgggcca gtgcgccgaa tttgtttaca aggagtcgga agaccttgct gacttacagg
    20281 ctattacgcc gccatcagaa caacatcatc gtttgcattt atctttgtgg tcagtttcta
    20341 aaaaaccgca aagtcgccaa ctctgacgaa aactatcgtt gtgctgccac aacgataaga
    20401 gcactcggtg catttaagcc aagccccata agggagaatg ctcttacctg ttacacagat
    20461 ataaaaaatc ccgaaaccgt tatgcaggct ctaactatta cctgcgaact gtttcgggat
    20521 tgcattttgc agacctctca gcctgcgatg gttggagttc cagacgatac gtcgaagtga
    20581 ccaactgggc ggaatcggta gtaagcgccg cctcttttta tctcaatacc acaacgagcg
    20641 aattaaccca tcgttgggtc aaatttaccc aactttattc aataagtcaa tatcatgccg
    20701 ttaatatgtt gccatccgtg gcaatcatgc tgctaacgtg tgaccgcgtt caaaatgttg
    20761 tctgcgattg actcttcctt gtggcattgc accaccagag cgtcatacag cggcttaaca
    20821 gtgcgtgacc aggtgggttg agtaaggttt gggattagca tcgttacagc gcgatatgcg
    20881 gcacttgctg gcatccttga atagccgacg cctttgcatc ttccgcactc tttctcaaca
    20941 actctccccc actgctctgt ttttgctata tcaaccgcac ggcctgtacc gtgacaatct
    21001 ctgcatcttg cgcccggcgt cgcggcacta cggcaataat ccgcataagc gaatgttgcg
    21061 agcacttgca gtacctttgc cttagtattt ccttcaagct ttgccacgcc acggtatttc
    21121 cccgatacct tgtgtgcaaa ttgcatcaga tagttgatag ccttttgttt gtcgttctgg
    21181 ctgagttcat gcttaccgca gaatgcagcc attccgaatc cggcttgtga ttgcgccatc
    21241 cccatagcag ccatcacatc agtaccggaa agagagtcag aagccgtagc ccgtggtgag
    21301 tcgctcatca tcgggctttt tggcgaatga aatttagcta cgctttcgag tctcatgcgc
    21361 cttctccctg tacatgaatc aatgtgaggt ttccgcagaa cactgcgccg gtatcgatat
    21421 acatctggtt ggcaaacttg agtggtttca ctgctggcgt atgaccaaag atgaacatgt
    21481 ccgctccttt gatttctttc acgatcccgt cttgtgagtt gctgattcgt tcgcggttcc
    21541 aaattaactg ctgatgatca actggctttc caaactcgta ttcgtcacaa ggataatcgg
    21601 cgtggcagat gacatatttt ttatctttgc tcaccagttc gatgattaac ggaagttca
//
LOCUS       DAZFWL020000077        21638 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFWL020000077 DAZFWL020000000
VERSION     DAZFWL020000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.077"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..307
                     /gene="cybC"
                     /locus_tag="ACHKNP_RS15105"
                     /old_locus_tag="pgaptmp_003021"
     CDS             <1..307
                     /gene="cybC"
                     /locus_tag="ACHKNP_RS15105"
                     /old_locus_tag="pgaptmp_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU4705149.1"
                     /translation="NMETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLE
                     DKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(352..816)
                     /gene="nrdG"
                     /locus_tag="ACHKNP_RS15110"
                     /old_locus_tag="pgaptmp_003022"
     CDS             complement(352..816)
                     /gene="nrdG"
                     /locus_tag="ACHKNP_RS15110"
                     /old_locus_tag="pgaptmp_003022"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HGU4705150.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(975..3113)
                     /gene="nrdD"
                     /locus_tag="ACHKNP_RS15115"
                     /old_locus_tag="pgaptmp_003023"
     CDS             complement(975..3113)
                     /gene="nrdD"
                     /locus_tag="ACHKNP_RS15115"
                     /old_locus_tag="pgaptmp_003023"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGU4705151.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(3507..5162)
                     /gene="treC"
                     /locus_tag="ACHKNP_RS15120"
                     /old_locus_tag="pgaptmp_003024"
     CDS             complement(3507..5162)
                     /gene="treC"
                     /locus_tag="ACHKNP_RS15120"
                     /old_locus_tag="pgaptmp_003024"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGU4705152.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(5212..6633)
                     /gene="treB"
                     /locus_tag="ACHKNP_RS15125"
                     /old_locus_tag="pgaptmp_003025"
     CDS             complement(5212..6633)
                     /gene="treB"
                     /locus_tag="ACHKNP_RS15125"
                     /old_locus_tag="pgaptmp_003025"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HGU4705153.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(6752..7702)
                     /gene="treR"
                     /locus_tag="ACHKNP_RS15130"
                     /old_locus_tag="pgaptmp_003026"
                     /pseudo
     CDS             complement(6752..7702)
                     /gene="treR"
                     /locus_tag="ACHKNP_RS15130"
                     /old_locus_tag="pgaptmp_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
     gene            7884..7937
                     /gene="mgtL"
                     /locus_tag="ACHKNP_RS15135"
                     /old_locus_tag="pgaptmp_003027"
     CDS             7884..7937
                     /gene="mgtL"
                     /locus_tag="ACHKNP_RS15135"
                     /old_locus_tag="pgaptmp_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HGU4705154.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8078..10774
                     /gene="mgtA"
                     /locus_tag="ACHKNP_RS15140"
                     /old_locus_tag="pgaptmp_003028"
     CDS             8078..10774
                     /gene="mgtA"
                     /locus_tag="ACHKNP_RS15140"
                     /old_locus_tag="pgaptmp_003028"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGU4705155.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(10957..11310)
                     /gene="yjeI"
                     /locus_tag="ACHKNP_RS15145"
                     /old_locus_tag="pgaptmp_003029"
     CDS             complement(10957..11310)
                     /gene="yjeI"
                     /locus_tag="ACHKNP_RS15145"
                     /old_locus_tag="pgaptmp_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HGU4705156.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11448..13094)
                     /gene="groL"
                     /locus_tag="ACHKNP_RS15150"
                     /old_locus_tag="pgaptmp_003030"
     CDS             complement(11448..13094)
                     /gene="groL"
                     /locus_tag="ACHKNP_RS15150"
                     /old_locus_tag="pgaptmp_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGU4705157.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13138..13431)
                     /gene="groS"
                     /locus_tag="ACHKNP_RS15155"
                     /old_locus_tag="pgaptmp_003031"
     CDS             complement(13138..13431)
                     /gene="groS"
                     /locus_tag="ACHKNP_RS15155"
                     /old_locus_tag="pgaptmp_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGU4705158.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13707..14963
                     /gene="yjeH"
                     /locus_tag="ACHKNP_RS15160"
                     /old_locus_tag="pgaptmp_003032"
     CDS             13707..14963
                     /gene="yjeH"
                     /locus_tag="ACHKNP_RS15160"
                     /old_locus_tag="pgaptmp_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HGU4705159.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(14979..15455)
                     /gene="fxsA"
                     /locus_tag="ACHKNP_RS15165"
                     /old_locus_tag="pgaptmp_003033"
     CDS             complement(14979..15455)
                     /gene="fxsA"
                     /locus_tag="ACHKNP_RS15165"
                     /old_locus_tag="pgaptmp_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HGU4705160.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            15792..17228
                     /gene="aspA"
                     /locus_tag="ACHKNP_RS15170"
                     /old_locus_tag="pgaptmp_003034"
     CDS             15792..17228
                     /gene="aspA"
                     /locus_tag="ACHKNP_RS15170"
                     /old_locus_tag="pgaptmp_003034"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HGU4705161.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17346..18647
                     /gene="dcuA"
                     /locus_tag="ACHKNP_RS15175"
                     /old_locus_tag="pgaptmp_003035"
     CDS             17346..18647
                     /gene="dcuA"
                     /locus_tag="ACHKNP_RS15175"
                     /old_locus_tag="pgaptmp_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HGU4705162.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            18763..19101
                     /gene="cutA"
                     /locus_tag="ACHKNP_RS15180"
                     /old_locus_tag="pgaptmp_003036"
     CDS             18763..19101
                     /gene="cutA"
                     /locus_tag="ACHKNP_RS15180"
                     /old_locus_tag="pgaptmp_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HGU4705163.1"
                     /translation="MLDEESSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            19077..20774
                     /gene="dsbD"
                     /locus_tag="ACHKNP_RS15185"
                     /old_locus_tag="pgaptmp_003037"
     CDS             19077..20774
                     /gene="dsbD"
                     /locus_tag="ACHKNP_RS15185"
                     /old_locus_tag="pgaptmp_003037"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HGU4705164.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEVKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20811..21386
                     /gene="yjdC"
                     /locus_tag="ACHKNP_RS15190"
                     /old_locus_tag="pgaptmp_003038"
     CDS             20811..21386
                     /gene="yjdC"
                     /locus_tag="ACHKNP_RS15190"
                     /old_locus_tag="pgaptmp_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4705165.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21493..21568
                     /locus_tag="ACHKNP_RS15195"
                     /old_locus_tag="pgaptmp_003039"
     tRNA            21493..21568
                     /locus_tag="ACHKNP_RS15195"
                     /old_locus_tag="pgaptmp_003039"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21526..21528,aa:Phe,seq:gaa)
BASE COUNT         5046 a         5630 c         5578 g         5384 t
ORIGIN      
        1 caatatggaa accctcaacg acaatttaaa agtgatcgaa aaagcggata acgcggcgca
       61 agtcaaagac gcgttaacga agatgcgcgc cgcagcgctg gatgcgcaaa aagcaacgcc
      121 gccgaagctc gaagataaat caccggacag cccggaaatg aaagatttcc gccacggttt
      181 cgacattctg gtcggtcaga ttgacgacgc gctgaagctg gcaaatgaag gtaaagtaaa
      241 agaagcgcag gctgctgcag agcaactgaa aacgacccgc aacgcctatc accagaagta
      301 tcgttaattc ctcatttccc tgttgcctgc actcaggtaa cagggaactt atcatcgcaa
      361 atgatgcacc acctgattgc tgctgccgcg ccagatcagg gaagggtctt ttaaatcctg
      421 cacaaatttg ccgtcgacca gtacgttaat caaatcaaca acctgcattt gcgcagcgtt
      481 gagttcgtcg agtttatagc ctgtccacac ccagatgtct ttacccggac actcggcgcg
      541 gatgcgtttt accagtttca gaatatccgg cacgttttgc ggatgcagcg gatcgccgcc
      601 ggagagggaa ataccctggc gtttgatacg agtgtcattc agatcgttaa taatctggtc
      661 ttccattgct ttagtaaatg tctgaccgga atttacccgc catgtgcttt tgttatagca
      721 accggggcat tcatgaacac acccggagac aaacagggtg cagcgagtgc cggggccgtt
      781 gacgatgtcg acaggatagt actgatgata attcattgtt tcgcctggct tatcagtccg
      841 cgtataggat gcatccggta atcaacgcct gatgcgacgc tgtcgcgtct tatcagacct
      901 acaatcgttg ttgccatgta ggccggataa ggcgtttacg ccgcatccgg catttgctta
      961 acgtagagtg aagattaacc tatctgccca ttccccaaat gtttaacgcg gcgcttaact
     1021 tcttcctgct taccggcgtt aaatggacgt gcatccgggc tacctaaata tccgcacacg
     1081 cggcgagtca ccgacacacg ggaggcgtca tggttaccac atttcgggca agtgaagcct
     1141 ttgctggtgc actcgaactc accggtaaag ccacactcgt agcactcatc aatcggtgta
     1201 ttggtgccgt aatacggtac atgctgatag ctgtaatccc agacatcttc cagcgccttc
     1261 aggttgtgct gaatgtttgg atactcgccg tagcaaatga aaccaccgtt cgccagcggc
     1321 gggtaaggcg cttcaaagtc gatcttgtcg tacgggttca ccttcttctc cacatcgagg
     1381 tggaaactgt tggtgtagta acctttatcg gtcacgcccg gcaccacgcc aaactcagca
     1441 gtatcgagac ggcagaagcg gtcgcacagg ttttcactcg gcgtgctgta gagactgaaa
     1501 ccataacccg tttcttcttt ccactgatcc actgcctggc gcagacgttc aacaatggcg
     1561 atacctttcg cgcgaagctg ctcgttgtcg taaacatgct cgccgccgaa cagcgcgtta
     1621 atggtttcgt ggatgccgat gtaacccagc gaaatagacg cacgaccgtt tttgaagatt
     1681 tcagaaacat catcgtcagc attcagacgt acgccacaag caccttccat atagaggatc
     1741 ggggccacgc gcgctttcac gccttcgaga cgagcgatac gggtcatcag cgccttacgt
     1801 gccagcacca gacgttcatc cagcagcttc cagaaggtgg cttcatcgcc ttttgcttcc
     1861 agagcaatac gcggcaggtt caggctgatc acgccgaggt tgttacgacc atcgtggatc
     1921 tgctcgccgt tttcattttc ccacacgccg aggaagctgc ggcagcccat cggggtttta
     1981 aacgaaccgg tgactttcac tacctgatcg tagttcagga tatccggata catgcgcttg
     2041 cttgcgcact ccagcgccag ctgtttgatg tcgtagttcg gatcgccttt tttatggttc
     2101 agaccatcgc gaatcgcaaa caccagtttc gggaacaccg cagttttacg gtttttaccg
     2161 aggcctgcga tacggttacg caggatcgat tcctgaatca ggcgcgattc ccagctggtg
     2221 cccagaccaa aaccaaaggt tacaaacggc gtctgaccgt tggcggtgtg cagggtgttt
     2281 acttcgtact ccagcgactg gaaggcatcg tagcactctt tgatggtacg agagtttgcg
     2341 tagccttcgg cgtccgggat gttccactct tctgcggtct tgcgatgttt gttgtagctg
     2401 gcggtaacaa acggcgccag cacttcatcg atacggttaa tggtggtgcc gccataaata
     2461 tggctggcga cctgagcaat aatctgcgca gttaccgcgg ttgccgtaga gatcgacttc
     2521 ggcggttcaa tctcggcgtt ccccatctta aagccctggg tcagcatgcc tttcaggtcg
     2581 atcaacatgc agttgaacat cgggaagaac ggcgagtaat cgagatcgtg atagtgaata
     2641 tcgccacgct catgtgcctg caccacgtca cgcggcagca ggtgctgacg tgcatagtgt
     2701 ttagccacga tcccggccag caggtcgcgc tgggttggaa tcaccttgct gtctttgttg
     2761 gcgttttcgt tgagtaacga ggcgttggtc tgctcgacca gaccacggat ctcctggttc
     2821 aggcgaccgc gtttttcacg ttcaatgtcg cgatcgtgac ggtactcgat gtaagcacga
     2881 gccagttgtt tgtatggacc cgacatcagc tggttttcaa ctgcggtctg gatctcgttg
     2941 atatccacct ggttgcggcc ctgcatctgc tcgctgacaa ccgcggcaac agtggcgcaa
     3001 taatcggcat catcgacttc cgctgcttta gctgcacgca gaatcgcttc tttgatgcgc
     3061 tctgatttaa acggcacttt gcagccgtct cgtttcatca catgcggtgt catgatcgct
     3121 ccatattttt aagaacaggt tatccacaga aattgggaaa ggcattttcc ggttttttcg
     3181 tttcgttttc cggtgctttc cgcaatcgcc atccgcttat ccacaacgcc gcaccttttt
     3241 cgtggggagc tgtagtagca attatagtcg attaatacaa catattgggt tgggacgcat
     3301 tttaaagtct atatatagtg ctttgcatca aggatgtttt aacttttttt gatgtagctc
     3361 aaagtaaaaa gcagagcgta cggatgacgg gcgctacagc gttatgtaaa ttttttaatg
     3421 aatttgctgg ttgaaaaatc aacaaaaaca acatgctgac agacaaaacc ccggaatgac
     3481 cggcattacc ggggcttagg gaagatttac ttctgtaacc accagacagc ctcaaaaggc
     3541 cgtaaattca tggcacaggg ttgtggtgag gcttcttcgt agttatgcat cacaagctgc
     3601 cagttgccgc gcatttgccc tggctgccag ggttggatct cacggctaag gttggcaatg
     3661 accagcaagg tttgcccctt ccattcacgg cgatagcacc acaatacagg gctgtttggc
     3721 agcagatcct ggtaattgcc ccatgtcagg atggcttctt gcttacgcag tgcgattaac
     3781 ttttggtagg tgtaaaacac cgaggagtca tcggccagcg cggcttctac gttgatttgt
     3841 tgatagttat cgcccaggcc aatccacggt tcgccagccg taaacccggc attatcgccg
     3901 ttgctccatt gcatgggcgt gcggctgttg tcacgggatt tactggcgag gattgccaat
     3961 aactcgtcgg catcacgacc atcgttgcgc agctcggcaa acatattgag gctctccacg
     4021 tcgcgatagt cagtaatgcg cgtgaaatgc gggttggtca tgccaatctc ttcgccttgg
     4081 tagatatacg gcgttccctg catgccatgc agcaccatcg ccagcatttt tgccgcaggc
     4141 acgcggtatt caccttcatc accaaagcga gaaacaatgc gcggctgatc gtggttacac
     4201 cagaacaagg cattccatgc cacgttgtgc attccttgtt gccagtggcg gaacaatgtt
     4261 ttcaacgcca caaagtcagg tttagccagc gtccattttt caccaccggg ataatcgacc
     4321 ttcaggtgat gaaaattaaa ggtcatcgac aattcactgc ctgtcagtgc cgcgtatcgc
     4381 tggcaatgct caaggctggt ggaggacatt tcacctacgg tcattaaccc gcgtggcgta
     4441 aacacatcgc ggttcatctc gtgcaaaaac tcgtgtgctc gtggcccgtc ggtgtagaag
     4501 cgacgcccgt cgccatccag gtcttcaggg aaacgcgggt ctttggagat cagattcacc
     4561 acatccaggc gcaacccgtc gaccccacga tcggcccaga attcacagac ttttttcagc
     4621 tctgcgcgta ccgctggatt ctcccagttg agatccgcct gttctggtgc aaagagatgc
     4681 aaatagtact gttcgctttc cgcatgccag cgccacgcac taccgccaaa ttttgaacgc
     4741 cagttgttcg gtggcgtttc tggttcgcca tcgcgccaga tataaaactg gcggtaaggg
     4801 ctttctttgt tcagcgcctc gcgaaaccag gcatgttggg tagaggtatg gttaaacacc
     4861 atatcgagaa tgatacgaat cccgcgcgat tttgcctgcg tcaccagttc gtcaaaatcg
     4921 tccagcgtgc cgtagatggg atcaatcgcc gtatagttcg ctacgtcgta accgttatcg
     4981 acctggggag agatataaaa gggggttagc cagatggcat caacgcccag tttatgcaga
     5041 tagtccaggc gttggataac gccacgtaaa tcgccggtgc cgctacccgt ggtgtcctga
     5101 aaactctttg gataaatctg gtagataacg ccgttttgcc accagtgggg aagattagtc
     5161 attacgttat tcctgcaaat gcgagggggc gcaattgcgc cccgaagaaa attaaacaat
     5221 atccagcgtg cccaggcggt atttccgctg atagataaac gaggtgagta caatcgggat
     5281 gatgatggcg ataaccattg ccagcgcaaa tacctgccag tagctcggtt gaatcgagag
     5341 aattcccggc aggccgccta cgccgatacc attcgccata acgccgttca ggccgcatag
     5401 taatcctgcc agaccagaac caatcatcgc gcacagcatc gggaagcgat atttcaggtt
     5461 gatgccgtac attgcaggct cagtgacccc aagccaggcg gagatagcgg caggcacgga
     5521 gatctcgcgt tcattgtgct tgcggctgga aatgataatg cctatcacgg cggagccctg
     5581 agcgatattc gacagcgcaa tcagcggcca cactggcgtg ccacccatgc tttgaatcat
     5641 ctgcaaatca atagcaaggg tggtctggtg tacaccggtg atcaccagcg gggcgtacag
     5701 gaagccaaac aatgctgcgc caatcggagc aaagctgccg gtcatcaggt gacgtaccgc
     5761 aaaggcaacg ccatcgccaa tcatgcgacc aaacggacca atcagcgcat gggcgaggaa
     5821 caccgcgagg atcagcgaac agacgggcac caccaccaga tagaggtaat ccggcacgat
     5881 gcgtttaagg cgagtttcaa taacgcccag cgccagcccg gctaacagtg ccgggatcac
     5941 ctgcgcctga tagcctactt tggcgatgct gaacatgcca aagtcccaca cttccggcag
     6001 ctgctgcccg agcagataag cgttcatcag ctggggagaa accagtgtca cgccaagcac
     6061 gataccaagg atcggcgtgc cgcccatttt tttcaccgct gaccagcaaa taccgaccgg
     6121 caggtagaag aagatcgctt caccgatcaa ccacagaaaa tcgtagatcg tttgcaggga
     6181 agggtacatt tgcgccagcg tctgaccgtt gctcatgggc aaatcgccga tcacattgcg
     6241 aaaaccgagg atcaaaccgc cgctaatcaa cgcgggcagc agcgggaaga agatctccgc
     6301 gaaatgagag atcaactgct catgccattt catattctgc cgggcggctt tttttacctg
     6361 ctctttatca acctgcgcct gtccggttga cgcaatcagt gcttgatagt aatcacccac
     6421 attggtgcca atcaccacct gaaattgccc ggcattggtg aaacagcctt tcaccatggg
     6481 gagttgctca atttctttcg gtctggcatt ggccggttgg ttgaggacaa agcgcaggcg
     6541 agtaatacag tggctcaccg tcgcaatatt gccgcgcccg ccgaccagtt caatcaaccg
     6601 atcgatatcc gtttggttta ttttgctcat cataaagccc catggcagat gacatttttg
     6661 gttggctgca gaatatattg cgcggaaaaa tttaaaaacg ggaacgttcc cgaaacacag
     6721 cgaagatcac aatttatcgt tcaggaaacg atcaggacag ggtggcgggg atgatgattt
     6781 gttgcggttc gctgcgcccg gttacctgtg cgatcaattg gcaggccgcc tggcgtccag
     6841 cttcggcgta accaggatct acggtgacga tctccggatg aaggaatttc attaacggcg
     6901 tattaccgac gctcgccagt tgcaaggtgt cgatgcgttg ctcttgcagg tatttacttg
     6961 cgccaagtgc cagcgtgtcg gttgcgcaca gtaaggcggt agtttcaggc gtaatcactt
     7021 ttgctacgtt ctcatagcct tgcttcatag caagccctgg cagagcggca acgggatgca
     7081 gtttatgcgc tttgcagaac gccaggtagg cttcgtgacg tcgcttaccg gtcgtcacgt
     7141 cactgtgcgg cacgccgaga taactgatat tacgatgccc ctggtcatag agccgttgca
     7201 tcaggatctt gattgcccct tcgtcgtcat aacagaccga agcaaagcct tttgcgtcac
     7261 gcgccagcag aaccagcgat gactgccagt gggctaacat ttcttctgtt atgccggtaa
     7321 aaccgaacag cactacgccg tcgatattac gccgttttag gatccccaaa tgttcggcaa
     7381 ctaattgcgg ggaaaactga ctttccatca tgattgggtc gtaaccttgt tcatagaacg
     7441 ctggcagcat ggtttgaacg gcgagatttt ctgacaacga atccaggcgg gtaacaatga
     7501 tggcgaccac tttatcgctt tgcccacgca tagcgcgcgc agagcgggaa ggggaaaatc
     7561 catgctgatt catcactgct tccacgcgct cgcgcgtgcg ctggctcacg ccgctttcgt
     7621 tattcagcac ccgggaaacc gtagatttcc ccacgccgct taagcgcgcg atgtctttga
     7681 tggtcagccg attttgcatc ctgttgtccc gtaacgtgtt gtttaattat ttgaggctaa
     7741 ctttacccgt gcattcagca atgggtaaag tctggtttat cgttggttta gttgccagca
     7801 ggtattatat cgccataaat gctacgaata ttattggatt ctccttatta tttgcggcgc
     7861 ttttttcact taccggaggt tatatggaac ctgatcccac gcctctccct cgacggagat
     7921 taaaactttt tcggtaagcc cgtcttttca cggcgttacc ggatgcgtaa ggccgtgacg
     7981 ttttaacgtc cctgctcagc tttattacct tcaggtaagg cttcgccacg cctgaagaca
     8041 tttctgtact gtttcagaca gtgcggaggg actccttatg tttaaagaaa tttttacccg
     8101 gctcattcgc catttacctt cccgtctggt tcatcgtgat ccattgcctg gcgcgcagca
     8161 gacagtgaat acggtggtcc cgccgtcctt aagtgcgcat tgcctgaaaa tggcggtgat
     8221 gcccgaagaa gaattgtgga aaacgttcga cacccatccg gaagggttaa atcaggcgga
     8281 agtggaatct gcccgcgaac aacatggtga aaataaatta cccgcacaac aaccgtcgcc
     8341 gtggtgggta catttatggg tctgctatcg caaccccttt aatattttac tcaccattct
     8401 cggcgctatt tcttacgcca cggaagattt atttgccgcg ggcgttatcg cgctaatggt
     8461 cgccatttct acgttgctga actttattca ggaagcacgt tccactaaag cggcagatgc
     8521 cctgaaagcg atggtcagca atactgcgac ggtgctgcgc gtaattaacg acaaaggcga
     8581 aaatggctgg ctggagatcc cgatcgacca gctggtgccc ggcgatatta taaaactggc
     8641 ggcgggggat atgatcccgg cagatttacg tatcttgcag gcgcgggatc tgttcgtcgc
     8701 tcaggcgtcg ttaaccggtg agtctctgcc cgtagaaaaa gcagctacca ctcgccagcc
     8761 ggagcacagc aatccgctgg agtgcgacac cctgtgtttt atgggcacca ccgtggtgag
     8821 cggcacggca caagcaatgg tgattgctac aggtgccaat acctggtttg gtcaactggc
     8881 ggggcgtgtt agtgagcagg aaagcgagcc gaatgccttt cagcaaggga tcagccgcgt
     8941 cagtatgctg ctgattcgct ttatgctggt gatggctccg gtggtgctgt taatcaatgg
     9001 ttacaccaaa ggcgactggt gggaagcggc actgtttgcg ctttcggtcg cggtaggctt
     9061 aacgccggaa atgttgccga tgattgttac ctcgacgctg gcgcgcggcg cagtaaagct
     9121 gtcgaaacag aaagtgatcg tcaaacatct ggatgctatt cagaactttg gcgcaatgga
     9181 tattctgtgc actgataaaa ctggcaccct gacacaggac aaaattgtgc tggagaatca
     9241 taccgatatc tccggtaaaa ccagcgaacg cgtgctgcat agcgcgtggt tgaacagtca
     9301 ttatcagacc ggacttaaaa acctgctcga tacagcggtg ctcgaaggta cggatgaaga
     9361 gtcagcgcgc tcgctggcca gtcgttggca aaaaatcgat gagattccgt ttgatttcga
     9421 gcgtcgccgg atgtcggtgg tagtggcaga aaataccgag caccatcagc tggtttgcaa
     9481 aggtgcattg caggaaatcc tcaatgtgtg ttcgcaggtg cgtcacaatg gcgagattgt
     9541 gccgctcgat gacatcatgc tgcgtaagat taagcgggtt actgatacgc tgaatcgtca
     9601 ggggctgcgc gtggttgcgg tggcgacgaa atacctgcca gcgcgtgaag gagattacca
     9661 gcgggcggat gaatccgacc tgatcctcga aggatatatt gctttccttg atccgccaaa
     9721 agagacaact gctccggcac tgaaggcatt aaaagcgagt ggaattaccg taaaaatcct
     9781 tactggcgac agtgagttag ttgcagcgaa agtgtgccat gaagtggggc tggacgcggg
     9841 agaggtggtc attggtagtg atattgaaac gctatctgac gacgaactgg caaatctcgc
     9901 acagcgcacc acgctgtttg ctcgtctgac gccgatgcat aaagaacgca ttgtgacctt
     9961 actgaagcgc gaagggcatg tggttggctt tatgggcgat ggtattaatg atgcgcccgc
    10021 cttacgcgct gcggatatcg gcatttctgt ggacggcgcg gtagatattg cccgtgaagc
    10081 ggctgatatc atcctgctgg aaaaaagcct gatggtgctg gaagaggggg ttattgaggg
    10141 acgtcgcact ttcgccaaca tgctgaaata catcaaaatg acggcgagct ctaacttcgg
    10201 taatgtgttc agcgtgctgg tggcgagtgc tttcttgccc ttcctgccga tgttgccgtt
    10261 acacttgctt attcagaacc tgctgtacga tgtgtcacag gtggcgatcc cgtttgataa
    10321 cgtcgacgac gagcaaattc aaaagccgca gcgttggaat ccggcggatc tggggcgctt
    10381 tatgatcttc ttcggaccga tcagctcgat cttcgatatt ctgacgtttt gcctgatgtg
    10441 gtgggtattc catgccaaca cgccggaaac gcaaacgctg ttccagtcgg gatggtttgt
    10501 ggtgggctta ctgtcgcaaa cgctgattgt gcatatgatc cgtacccgcc gtgtgccgtt
    10561 tattcagagc tgtgcatcgt ggccgttaat gatcatgacc gtgatcgtga tgattgtcgg
    10621 gatcgcattg ccgttttcac cgctggccag ttatctgcag ctgcaggcgc tgccgttaag
    10681 ctatttcccg tggctggttg cgattctggc agggtatatg acgttaaccc agttggtgaa
    10741 agggttctat agccgtcgtt acggctggca gtaaagaata aactgggcac gatagccccg
    10801 attcagattg cagacaaagt gagcgttgtt catgccggat gcggcgtaaa tgccttatcc
    10861 ggcctacaaa ttcaatcaat tgcaggaatc acgtagatac tgataagcga agcgcatcag
    10921 gcaatgatac gtttgtcacc cagcctctgg atcacatcag ttcgggcact tataaacctg
    10981 accgataatc tggctatccg tcgggacaaa actggacaac attccctgcg acgggctgct
    11041 gatgccataa atcacgttac cgcccattgc agccgcctgg ttgcgcagat cgtttgctgc
    11101 gccgcgcata gaaccgccct cttctccgtg ttgcccggaa agccagttgc tttgcttacc
    11161 tgtcgcagta ccaatcagct ggcactctgc gcctggctgc tcgtccacaa tgcgtacact
    11221 ctgaccggca gcactcaatt cgttgctgga gctacaaccc gccatcagta gcgcggcacc
    11281 gacaatccct gctaagtatt ttacgtgcat gttattcccc ataatcaatg agctggacgc
    11341 cactcgcgtc gtccgtgtct gaatcttata ctagaaagat gaccaaaaga aaaaccccca
    11401 gacatttctg cccgggggtt tgtttatttc tgcgaggtgc agggcaatta catcatgccg
    11461 cccatgccac ccatgccgcc cataccgcca gcagcgccta agtcagctgc atcatttttc
    11521 ggcaggtcgg taaccatgca ctcggtggtg atcatcaggc cagccacaga agccgcgtac
    11581 tgcagagcag aacgggttac tttggttggg tccaggatac ccatgtcgat catgttgccg
    11641 tattcttcgg ttgctgcgtt gtaaccgtag ttgccgtcgc cgcctttaac ggtgttagca
    11701 acaacagacg gttcttcgcc gcagttcagg acgatctgac gcagcggagc ttccattgca
    11761 cgcagtgcaa ctttgatacc cacgttctgg tcttcgttct gaccacgcag gtcagccagt
    11821 ttagacgcta cgcggatcag cgcaacacca ccaccagcaa ccacgccttc ttctaccgca
    11881 gcacgggtcg cgtgcagggc gtcttcaacg cgtgcttttt tctctttcat ttcaacttcg
    11941 gtagcagcac ctactttgat aactgcaacg ccgcctgcca gtttcgctac gcgttcctgc
    12001 agtttttcac ggtcgtagtc agaagttgct tcttcaatct gctgacggat ctgagcaaca
    12061 cggccctgga ttgcagcttc ttcacccacg ccatcgatga tagtggtggt gtctttgttg
    12121 atcacaacac gtttagcctg acccaggtct tccagggttg ctttttccag ctccataccg
    12181 atctcttcag agattacggt accgccagtc agggttgcga tatcctgcag catagcttta
    12241 cgacgatcgc cgaagcccgg agctttaacc gcagcaactt tcacgatgcc acgcatggtg
    12301 ttaacaacca gagttgccag cgcttcgcct tcaacatctt cagcgatgat cagcagcggt
    12361 ttgcctgctt tggcaacagc ttccagaacc ggcagcattt cgcggatgtt agagattttc
    12421 ttgtcagcca gcaggatgaa cgggctttcc agttctactg cgccagtttc cggcttgttg
    12481 atgaagtaag gagacaggta gccacggtcg aactgcatac cttcaaccac gtccagttcg
    12541 tcctgcagac cggtaccgtc ttcaacggtg ataacgcctt ctttaccgac tttgtccatc
    12601 gcttcagcga tcagtttacc tacggtttcg tcggagttag cggagatggt accaacctga
    12661 gcaatcgctt tagagtcaga gcacggtacg gacagcgctt tcagttcttc aactgcagcg
    12721 gtaacggctt tgtcgatacc acgtttcagg tccatcgggt tcatgcccgc agcaacagct
    12781 ttcagaccct cagtgatgat agcctgagcc agtacagttg cagtggtggt accgtcgcct
    12841 gcagcgtcgt tcgctttaga ggcaacttct ttcaccatct gcgcacccat attttcgaac
    12901 ttgtcttcca gttcgatttc acgagcaacg gaaacaccat ctttggtgat ggtcggtgca
    12961 ccgaaagatt tatccagaac tacgttacgg cctttcggac cgagggtaac tttcactgca
    13021 tctgccagta cgtttacgcc gcgcagcatt ttcacacgag cgtcgttacc gaattttacg
    13081 tctttagctg ccattatctt tattccttaa attcgtatgt tcagtgtcgt gcgaggatta
    13141 cgcttcaaca attgccagaa tgtcgctttc ggacatgatc aacacttctt cattgtcgat
    13201 cttctcagat ttcacaccgt agccatcgtt gaaaataacg atgtcgccaa ctttcacatc
    13261 cagcggcttc acttcgccat tttcaaggat acggccattg ccgacagcca gcacttcgcc
    13321 gcgggtggat ttagccgctg cagagccggt cagaacgatg ccgccagcag atttagtttc
    13381 aacttcttta cgcttgacga tcacgcgatc atgcaatgga cgaatattca ttgataactc
    13441 tcctttgaga aagtccgtat ctgttatggg tgacgccgga gcttacgtgg tttcccggct
    13501 ggtgaccaga gaaatgggga tgaggattcg ccccttcaag ggggaaaaag aaaaaaaatt
    13561 ctgatcagca caaaatcggg tgaaaaccct gattcacctc acatttcatc gcaatttctt
    13621 catcgccgtc ataagcgaat ctgattgtgc taccatcgaa aatctacgca gttgcccaaa
    13681 atttgggcgc aatcggatat caacccatga gtggactcaa acaagaactg gggctggccc
    13741 aaggcattgg cctgctatcg acgtcattat taggcactgg cgtgtttgcc gttcctgcgt
    13801 tagctgcgct ggtagcgggc aataacagcc tgtgggcgtg gcccgttttg attatcttag
    13861 tgttcccgat tgcgattgtg tttgcgattc tgggtcgcca ctatcccagc gcaggcggtg
    13921 tcgcgcactt cgtcggtatg gcgtttggtt cgcgacttga gcgagtcacc ggctggttgt
    13981 ttttatcggt cattcccgtg ggtttgcctg ccgcgctaca aattgccgcc gggttcggcc
    14041 aggcaatgtt tggctggcat agctggcaac tgttgttggc agaactcggt acgctggcgc
    14101 tggtgtggta tatcggtact cgcggtgcca gttccagtgc taatctacaa accgttattg
    14161 ccggacttat cgtcgcgctg attgtcgcta tctggtgggc gggcgatatc aaacctgcga
    14221 atatcccctt cccggcacct ggtaatatcg aacttaccgg gttatttgct gcgttatcag
    14281 tgatgttctg gtgttttgtc ggtctggaag catttgccca tcttgcctcg gaatttaaaa
    14341 atccagagcg tgattttcct cgcgcattga tgattggtct gctgctggca ggattagtct
    14401 actggggctg tacggtagtc gtcttacact tcgacgccta tggtgaaaaa atggcggcgg
    14461 cagcatcgct tccaaaaatt gtagtgcagt tgttcggtgt aggagcgtta tggattgcct
    14521 gcgtgattgg ctatctggcc tgctttgcca gtctcaacat ttatatacag agcttcgccc
    14581 gcctggtctg gtcgcaggcg caacataatc ctgaccacta cctggcacgc ctctcttctc
    14641 gccatatccc gaataatgcc ctcaatgcgg tgctcggctg ctgtgtggtg agcacgttgg
    14701 tgattcatgc tttagagatc aatctggacg ctcttattat ttatgccaat ggcatcttta
    14761 ttatgattta tctgttatgc atgctggcag gctgtaaatt attgcaagga cgttatcgac
    14821 tactggcggt ggttggcggg ctgttatgcg ttctgttact ggcaatggtc ggctggaaaa
    14881 gtctctatgc gctgatcatg ctggcggggt tatggctgtt gctgccaaaa cgaaaaacgc
    14941 cggaaaatgg cataaccaca taatccggcg tttcgacatt aatcctggcg atcgtcttta
    15001 tgatcaaggc ggtcgcgctc atcatccttt cgctggtact caccatcaaa agtattaccg
    15061 ccaccggtcc cggcgctaaa accgccgcct ggcatacgag aaaagcgcaa atgcggcatc
    15121 aacttcactg tcagatgctt ttgcaccggc ggtaataaaa gcagaagacc gaggaagtcg
    15181 gtgaaaaagc ccggtaataa aagcagcaaa ccggcaatga tcagcgaaac acttttaatc
    15241 atctccgccg ctgggttttc acccgccgcc attttttgct gcatcagcac gaaattctta
    15301 aagccctggt tacgtaccag tgacatcccg ataactgacg tgaatataac cagcacgagg
    15361 gtcagcaata cccccaatac atgggcaacc tgaataaaga ttgaaatctc aatatagaca
    15421 taaaggaaaa tggcaataaa aggtaaccag cgcaaaggtt tctcctgtaa tagcagccgg
    15481 taaaacccgg ctacctgaat gggttgcgaa tcgcgttcag cttatattgt ggtcattagc
    15541 aaaatttcaa gatgtttgcg caactatttt tggtagtaat cccaaagcgg tgatctattt
    15601 cacaaattaa taattaaggg gtaaaaaccg acacttaaag tgatccagat tacggtagaa
    15661 atcctcaagc agcatatgat ctcgggtatt cggtcgatgc aggggataat cgtcggtcga
    15721 aaaacattcg aaaccacata tattctgtgt gtttaaagca aatcattggc agcttgaaaa
    15781 agaaggttca catgtcaaac aacattcgta tcgaagaaga tctgttgggt accagggaag
    15841 ttccagctga tgcctactat ggtgttcaca ctctgagagc gattgaaaac ttctatatca
    15901 gcaacaacaa aatcagtgat attcctgaat ttgttcgcgg tatggtaatg gttaaaaaag
    15961 ccgcagctat ggcaaacaaa gagctgcaaa ccattcctaa aagtgtagcg aatgccatca
    16021 ttgccgcatg tgatgaagtc ctgaacaacg gaaaatgcat ggatcagttc ccggtagacg
    16081 tctaccaggg cggcgcaggt acttccgtaa acatgaacac caacgaagtg ctggccaata
    16141 tcggtctgga actgatgggt caccagaaag gtgaatatca gtacctgaac ccgaacgacc
    16201 atgttaacaa atgtcagtcc actaacgacg cctacccgac cggtttccgt atcgcagttt
    16261 actcttccct gattaagctg gtagatgcga ttaaccaact gcgtgaaggc tttgaacgta
    16321 aagctgtcga attccaggac atcctgaaaa tgggtcgtac ccagctgcag gacgcagtac
    16381 cgatgaccct cggtcaggaa ttccgcgctt tcagcatcct gctgaaagaa gaagtgaaaa
    16441 atatccaacg taccgctgaa ctgctgctgg aagttaacct tggcgcaaca gcaatcggta
    16501 ctggtctgaa cacgccgaaa gagtactctc cgctggcagt gaaaaaactg gctgaagtca
    16561 ctggcttccc atgcgtaccg gctgaagacc tgatcgaagc gacctctgac tgcggcgctt
    16621 atgttatggt tcacggcgcg ctgaaacgcc tggctgtgaa gatgtccaaa atctgtaacg
    16681 acctgcgctt gctctcttct ggtccacgtg ccggcctgaa cgagatcaac ctgccggaac
    16741 tgcaggcggg ctcttccatc atgccagcta aagtaaaccc ggttgttccg gaagtggtta
    16801 accaggtatg cttcaaagtc atcggtaacg acaccactgt taccatggca gcagaagcag
    16861 gtcagctgca gttgaacgtt atggagccgg tcattggcca ggccatgttc gaatccgttc
    16921 acattctgac caacgcttgc tacaacctgc tggaaaaatg cattaacggc atcactgcta
    16981 acaaagaagt gtgcgaaggt tacgtttaca actctatcgg tatcgttact tacctgaacc
    17041 cgttcatcgg tcaccacaac ggtgacatcg tgggtaaaat ctgtgccgaa accggtaaga
    17101 gtgtacgtga agtcgttctg gaacgcggtc tgttgactga agcggaactt gacgatattt
    17161 tctccgtaca gaatctgatg cacccggctt acaaagcaaa acgctatact gatgaaagcg
    17221 aacagtaatc gtacagggta gtacaaataa agaaggcacg tcagatgacg tgcctttttt
    17281 cttgtgagca gtaacttaaa aataacaacc taatatcaac ttgttaaaaa acaaggaagg
    17341 ctaatatgct agttgtagaa ctcatcatag ttttgctggc gatcttcttg ggcgccagat
    17401 tggggggaat aggtattggt tttgcaggcg gattgggggt gctggttctt gccgctattg
    17461 gcgttaaacc cggtaacatc ccgttcgatg tcatttccat tatcatggcg gttatcgccg
    17521 ctatttctgc catgcaggtt gctggcggtc tggactatct ggttcatcag acagaaaagc
    17581 tgctgcgccg taacccgaaa tacatcacga tcctcgcacc gatcgtgacc tatttcctga
    17641 ctatctttgc tggtactggc aacatatctc tggcgacact gccagttatc gctgaagttg
    17701 cgaaggaaca aggcgtcaaa ccttgccgtc cgctgtctac tgcagtggta tccgcgcaga
    17761 ttgcgatcac cgcatcgcca atctcagcgg cagtggttta catgtcttcc gtgatggaag
    17821 gtcatggcat cagctacctc catctgctct ccgtggtcat cccgtccacc ctgctggcgg
    17881 ttctggtgat gtcctttctg gtcactatgc tgttcaactc caaactctct gacgatccga
    17941 tttatcgcaa gcgtctggaa gagggcctgg ttgaactgcg cggtgaaaag cagattgaaa
    18001 tcaaatccgg tgcaaaaacg tccgtctggc tgttcctgct gggcgtagtt ggcgtggtta
    18061 tctatgcaat catcaacagc ccaagcatgg gtctggttga aaaaccgctg atgaacacca
    18121 ccaacgcaat cctgatcatc atgctcagcg ttgcaactct gaccaccgtt atctgtaaag
    18181 tcgataccga caacatcctc aactccagca ccttcaaagc aggtatgagc gcctgtattt
    18241 gtatcctggg tgttgcgtgg ctgggcgata ctttcgtttc caacaacatc gactggatca
    18301 aagataccgc tggtgaagtg attcagggtc atccgtggct gctggccgtc atcttcttct
    18361 ttgcttctgc tctgctgtac tctcaggctg caaccgcaaa agcactgatg ccgatggccc
    18421 tggcactgaa cgtttcaccg ctgaccgctg ttgcttcttt cgctgcggtg tctggtctgt
    18481 tcattctgcc gacctacccg acgctggttg ctgcggtaca gatggatgac acgggtacaa
    18541 cccgtatcgg taaattcgtc ttcaaccatc cgttcttcat cccgggtact ctgggtgttg
    18601 ccctggccgt ttgcttcggc ttcgtgctgg gtagcttcat gctgtaatga ccaatcgcgg
    18661 ggcgttcacg ccccgctttc tttcccgccg actaacatcc ttcccccgtc cgttgtatag
    18721 tgacctctct cttgcggttc catctgttct tgcgaggtgt ttatgcttga tgaagaaagt
    18781 tcgaataccg cgtctgtcgt ggtgctatgt accgcaccgg atgaagcgac agcccaggat
    18841 ttagccgcca aagtgctggc ggaaaaactg gcggcctgcg cgaccttgat ccccggcgct
    18901 acctctctct attactggga aggtaagctg gagcaagaat acgaagtgca gatgatttta
    18961 aaaactaccg tatctcacca gcaggcactg ctggaatgcc tgaagtctca tcatccatat
    19021 caaaccccgg aacttctggt tttacctgtt acacacggag acacagatta cctctcatgg
    19081 ctcaacgcat ctttacgctg atcctgctac tttgcagcac ttccgttttt gccggattat
    19141 tcgacgcgcc gggacgttca cattttgtcc ccgcggatca agcctttgct tttgattttc
    19201 agcaaaacca acatgacctg aatctgacct ggcagatcaa agacggttac tacctctacc
    19261 gtaaacagat ccgcattacg ccggaacacg cgaaaattgc cgacgtgcag ctgccgcaag
    19321 gcgtctggca tgaagatgag ttttacggca aaagcgagat ttaccgcgat cggctgacgc
    19381 ttcccgtaac catcaaccag gcgagtgcgg gagcgacgtt aactgtcacc taccagggct
    19441 gtgctgatgc cggtttctgt tatccgccag aaaccaaaac cgttccgtta agcgaagtgg
    19501 tagccaacaa cgcagcgtca cagcctgtgt ctgttccgca gcaagagcag cacaccgcgc
    19561 aattgccctt ttccgcgctc tgggcgttgt tgatcggtat tggtatcgcc tttacgccat
    19621 gcgtgctgcc aatgtaccca ctgatttctg gcatcgtgct gggtggtaaa cagcggctct
    19681 ccactgccag agcattgttg ctgaccttta tttatgtgca ggggatggca ctgacttaca
    19741 cggcgctggg tctggtagtt gccgccgcag ggttacagtt ccaggcggcg ctacagcacc
    19801 catacgtgct cattggcctc gccatcgtct ttaccttgct ggcgatgtca atgtttggct
    19861 tgtttactct acaactcccc tcttcgctgc aaacacgtct cacgttgatg agcaatcgcc
    19921 aacagggcgg ctcacctggc ggtgtgtttg ttatgggggc gattgccgga ctgatctgtt
    19981 caccatgcac caccgcaccg cttagcgcga ttctgctgta tatcgcccaa agcgggaaca
    20041 tgtggctggg cggcggcacg ctttatctct atgcgttggg catgggcctg ccgctgatgc
    20101 taattaccgt ctttggtaac cgcttgctgc cgaaaagcgg cccgtggatg gaacaagtca
    20161 aaaccgcgtt tggttttgtg atcctcgcac tgccggtctt cctgctggag cgagtgattg
    20221 gtgatgtatg gggattacgc ttgtggtcgg cgctgggtgt cgcattcttt ggctgggcct
    20281 ttatcaccag cctacaggct aaacgcggct ggatgcgcgt ggtgcaaata atcctgctgg
    20341 cagcggcatt ggttagcgtg cgcccacttc aggattgggc atttggtgcg acgcataccg
    20401 cgcaaactca gacgcatctc aactttacac aaatcaaaac ggtagatgag ttaaatcagg
    20461 cgctcgttga agtcaaaggc aaaccggtga tgttagatct ttatgccgac tggtgcgtcg
    20521 cctgtaaaga gtttgagaaa tacaccttca gcgacccgca ggtgcaaaaa gcgttagcag
    20581 acacggtctt acttcaggcc aacgtcacgg ccaacgacgc acaagatgtg gcactgttaa
    20641 agcatcttaa tgtccttggc ctaccgacaa ttctcttttt tgacggacaa ggccaggagc
    20701 atccacaagc acgcgtcacg ggctttatgg atgctgaaac cttcagcgca catttgcgcg
    20761 atcgccaacc gtgaacgaca cttgcagtgg gaaagacgga ggaaaatagc gtgcaacgtg
    20821 aagatgtact gggagaagcc ctgaaattat tagaattaca agggattgcc aacaccacgc
    20881 tggagatggt tgctgaacgt gtggattatc cactggacga gctacgccgc ttctggccag
    20941 acaaagaggc aatcctctac gatgcgctgc gttatcttag ccaacagata gatgtctggc
    21001 gtcgtcagct gatgctggac gaaacgcaaa ccgccgaaca aaagctgctg gcacgttatc
    21061 aggcgttatc ggagtgcgtt aaaaacaacc gctatccggg ctgtctattt atcgctgcct
    21121 gtacgtttta tcccgatcct ggccacccta ttcatcaact ggccgatcag caaaaaagcg
    21181 cggcctacga tttcacccac gaactgttaa ccacgctgga agttgacgat ccggcgatgg
    21241 tagcaaagca gatggaactg gtgctggaag gctgtttaag ccgaatgctg gtgaatcgta
    21301 gccaggcgga tgtcgacact gcacatcggc tggcggaaga tatcctgcgc ttcgcccgct
    21361 gccgccaggg tggtgcattg acctgacaga aacacagaaa agaagcgatt tgccgcaatc
    21421 ttaagcagtt gaatcgcttt tactgaaatt aggttgacga gatgtgcaga ttacggttta
    21481 atgcgccccg ttgcccggat agctcagtcg gtagagcagg ggattgaaaa tccccgtgtc
    21541 cttggttcga ttccgagtcc gggcaccaaa ttcatatcaa cggacctcca cggaggtccg
    21601 tttttcgttt cagaacacca tgatttaagt gttcttcc
//
LOCUS       DAZFWL020000078        21477 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFWL020000078 DAZFWL020000000
VERSION     DAZFWL020000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.078"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..85)
                     /locus_tag="ACHKNP_RS15200"
                     /old_locus_tag="pgaptmp_003040"
     CDS             complement(<1..85)
                     /locus_tag="ACHKNP_RS15200"
                     /old_locus_tag="pgaptmp_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGU4705166.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMA"
     gene            complement(85..1293)
                     /gene="ybjJ"
                     /locus_tag="ACHKNP_RS15205"
                     /old_locus_tag="pgaptmp_003041"
     CDS             complement(85..1293)
                     /gene="ybjJ"
                     /locus_tag="ACHKNP_RS15205"
                     /old_locus_tag="pgaptmp_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4705167.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            1377..1913
                     /gene="rcdA"
                     /locus_tag="ACHKNP_RS15210"
                     /old_locus_tag="pgaptmp_003042"
     CDS             1377..1913
                     /gene="rcdA"
                     /locus_tag="ACHKNP_RS15210"
                     /old_locus_tag="pgaptmp_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HGU4705168.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(2088..3773)
                     /gene="ybjL"
                     /locus_tag="ACHKNP_RS15215"
                     /old_locus_tag="pgaptmp_003043"
     CDS             complement(2088..3773)
                     /gene="ybjL"
                     /locus_tag="ACHKNP_RS15215"
                     /old_locus_tag="pgaptmp_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HGU4705169.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            4043..4420
                     /gene="ybjM"
                     /locus_tag="ACHKNP_RS15220"
                     /old_locus_tag="pgaptmp_003044"
     CDS             4043..4420
                     /gene="ybjM"
                     /locus_tag="ACHKNP_RS15220"
                     /old_locus_tag="pgaptmp_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HGU4705170.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(4450..4707)
                     /gene="grxA"
                     /locus_tag="ACHKNP_RS15225"
                     /old_locus_tag="pgaptmp_003045"
     CDS             complement(4450..4707)
                     /gene="grxA"
                     /locus_tag="ACHKNP_RS15225"
                     /old_locus_tag="pgaptmp_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HGU4705171.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            4867..5154
                     /gene="ybjC"
                     /locus_tag="ACHKNP_RS15230"
                     /old_locus_tag="pgaptmp_003046"
     CDS             4867..5154
                     /gene="ybjC"
                     /locus_tag="ACHKNP_RS15230"
                     /old_locus_tag="pgaptmp_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HGU4705172.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            5138..5860
                     /gene="nfsA"
                     /locus_tag="ACHKNP_RS15235"
                     /old_locus_tag="pgaptmp_003047"
     CDS             5138..5860
                     /gene="nfsA"
                     /locus_tag="ACHKNP_RS15235"
                     /old_locus_tag="pgaptmp_003047"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HGU4705173.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            5921..6823
                     /gene="rimK"
                     /locus_tag="ACHKNP_RS15240"
                     /old_locus_tag="pgaptmp_003048"
     CDS             5921..6823
                     /gene="rimK"
                     /locus_tag="ACHKNP_RS15240"
                     /old_locus_tag="pgaptmp_003048"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HGU4705174.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            6911..7387
                     /gene="ybjN"
                     /locus_tag="ACHKNP_RS15245"
                     /old_locus_tag="pgaptmp_003049"
     CDS             6911..7387
                     /gene="ybjN"
                     /locus_tag="ACHKNP_RS15245"
                     /old_locus_tag="pgaptmp_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HGU4705175.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            7738..8850
                     /gene="potF"
                     /locus_tag="ACHKNP_RS15250"
                     /old_locus_tag="pgaptmp_003050"
     CDS             7738..8850
                     /gene="potF"
                     /locus_tag="ACHKNP_RS15250"
                     /old_locus_tag="pgaptmp_003050"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HGU4705176.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            8945..10078
                     /gene="potG"
                     /locus_tag="ACHKNP_RS15255"
                     /old_locus_tag="pgaptmp_003051"
     CDS             8945..10078
                     /gene="potG"
                     /locus_tag="ACHKNP_RS15255"
                     /old_locus_tag="pgaptmp_003051"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HGU4705177.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGIDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            10088..11041
                     /gene="potH"
                     /locus_tag="ACHKNP_RS15260"
                     /old_locus_tag="pgaptmp_003052"
     CDS             10088..11041
                     /gene="potH"
                     /locus_tag="ACHKNP_RS15260"
                     /old_locus_tag="pgaptmp_003052"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HGU4705178.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            11038..11883
                     /gene="potI"
                     /locus_tag="ACHKNP_RS15265"
                     /old_locus_tag="pgaptmp_003053"
     CDS             11038..11883
                     /gene="potI"
                     /locus_tag="ACHKNP_RS15265"
                     /old_locus_tag="pgaptmp_003053"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HGU4705179.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            11943..12431
                     /gene="ybjO"
                     /locus_tag="ACHKNP_RS15270"
                     /old_locus_tag="pgaptmp_003054"
     CDS             11943..12431
                     /gene="ybjO"
                     /locus_tag="ACHKNP_RS15270"
                     /old_locus_tag="pgaptmp_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HGU4705180.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            12472..13599
                     /gene="rlmC"
                     /locus_tag="ACHKNP_RS15275"
                     /old_locus_tag="pgaptmp_003055"
     CDS             12472..13599
                     /gene="rlmC"
                     /locus_tag="ACHKNP_RS15275"
                     /old_locus_tag="pgaptmp_003055"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HGU4705181.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(13774..14505)
                     /gene="artJ"
                     /locus_tag="ACHKNP_RS15280"
                     /old_locus_tag="pgaptmp_003056"
     CDS             complement(13774..14505)
                     /gene="artJ"
                     /locus_tag="ACHKNP_RS15280"
                     /old_locus_tag="pgaptmp_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HGU4705182.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(14797..15465)
                     /gene="artM"
                     /locus_tag="ACHKNP_RS15285"
                     /old_locus_tag="pgaptmp_003057"
     CDS             complement(14797..15465)
                     /gene="artM"
                     /locus_tag="ACHKNP_RS15285"
                     /old_locus_tag="pgaptmp_003057"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HGU4705183.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(15465..16181)
                     /gene="artQ"
                     /locus_tag="ACHKNP_RS15290"
                     /old_locus_tag="pgaptmp_003058"
     CDS             complement(15465..16181)
                     /gene="artQ"
                     /locus_tag="ACHKNP_RS15290"
                     /old_locus_tag="pgaptmp_003058"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HGU4705184.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(16188..16919)
                     /gene="artI"
                     /locus_tag="ACHKNP_RS15295"
                     /old_locus_tag="pgaptmp_003059"
     CDS             complement(16188..16919)
                     /gene="artI"
                     /locus_tag="ACHKNP_RS15295"
                     /old_locus_tag="pgaptmp_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HGU4705185.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(16937..17665)
                     /gene="artP"
                     /locus_tag="ACHKNP_RS15300"
                     /old_locus_tag="pgaptmp_003060"
     CDS             complement(16937..17665)
                     /gene="artP"
                     /locus_tag="ACHKNP_RS15300"
                     /old_locus_tag="pgaptmp_003060"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HGU4705186.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(17883..18398)
                     /gene="ybjP"
                     /locus_tag="ACHKNP_RS15305"
                     /old_locus_tag="pgaptmp_003061"
     CDS             complement(17883..18398)
                     /gene="ybjP"
                     /locus_tag="ACHKNP_RS15305"
                     /old_locus_tag="pgaptmp_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4705187.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            18524..18847
                     /gene="ybjQ"
                     /locus_tag="ACHKNP_RS15310"
                     /old_locus_tag="pgaptmp_003062"
     CDS             18524..18847
                     /gene="ybjQ"
                     /locus_tag="ACHKNP_RS15310"
                     /old_locus_tag="pgaptmp_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HGU4705188.1"
                     /translation="MQFSTTPTLEGQIIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            18844..19674
                     /gene="amiD"
                     /locus_tag="ACHKNP_RS15315"
                     /old_locus_tag="pgaptmp_003063"
     CDS             18844..19674
                     /gene="amiD"
                     /locus_tag="ACHKNP_RS15315"
                     /old_locus_tag="pgaptmp_003063"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HGU4705189.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(19671..20684)
                     /gene="ybjS"
                     /locus_tag="ACHKNP_RS15320"
                     /old_locus_tag="pgaptmp_003064"
     CDS             complement(19671..20684)
                     /gene="ybjS"
                     /locus_tag="ACHKNP_RS15320"
                     /old_locus_tag="pgaptmp_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU4705190.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWTV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(20783..>21477)
                     /locus_tag="ACHKNP_RS15325"
                     /old_locus_tag="pgaptmp_003065"
     CDS             complement(20783..>21477)
                     /locus_tag="ACHKNP_RS15325"
                     /old_locus_tag="pgaptmp_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HGU4705191.1"
                     /translation="LNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDDA
                     VRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVNQI
                     GGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQLT
                     LLFGMKAPGLGRLCFSLEDKGDYSTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMA
                     KQIARLAEQSTD"
BASE COUNT         5197 a         5415 c         5648 g         5217 t
ORIGIN      
        1 gagccataaa ccgctcacgg ttataggttt tttgatcgct taagaaagta ccatccatgt
       61 ctaccgcaat taatttaatg ctcatcaact attctccatc gccgtctgcg ttttggtatc
      121 gggtttggcg acggctttcg cgacaatagc cgcgagaata accagcgcca gtacaaccag
      181 cattgcacta cgtaatccat aatgttcgcc gagatagccc agcagcggcg gcccgacgag
      241 gaaagccaga taaccggtcg ttgctaccac actgacgcgg gttggtgcat cggggccggt
      301 atcgctggcg gcagaaatgg tcagcgggaa gcccagcgag gcacccagtc cccagagtac
      361 aacagacacc ccagcgaccc aggcgctatc gacaaaaata atcagcccaa tacccaacgc
      421 tcccattagt gcactggccc gaaccacggc aacgcgactg taacggtcga tgaaccaacc
      481 gccggtaaag cgtccaacag tcatccccag ggtaaaaccg gcataaatca gcgagccgga
      541 agtagggcta aaaccgtgac cgtcaaccat taataagggt aaccagtcgt tggcagaacc
      601 ttcggcaaag gccatcgcca gcaccacaac accaatcagc agcaactgga tatcgcgata
      661 aaaaggtacg cctttttcac catgctgggt gccatcggca gcatttttgc ccgtaccgtc
      721 agggattgcc tgaatggcga tataaatagg cgcgattcct accagcgccg ccaataaaat
      781 gtgcaccgtt gccggaacgc caaaggccgt cagtgccatc ccgacaccag cgcctgccag
      841 cgtgcccagg ctataaaaac cgtgcatcat cggcaaaacc gttttattca tttctcgctc
      901 aacggcggca ccttcgacgt ttatcgccac ttccgcagaa ccaaaacttg ccccaaagac
      961 gccgaggcca accgcgaaga gcaggggtga tgtcagccag agtgccagac ttaatatcat
     1021 catcccgatc aatgcgcagg acatcgtgac caggatgaca ttacgtgtcc caaagcgttt
     1081 cactaaccac gccgagcaga gaataccgct catcgaaccg atcgacagac caaagagaac
     1141 accgcccatt tcagcgatcg agacagagag aatatcgcgg atagcaggcg tacgggttgc
     1201 ccaggacgcc attaacaggc ccggcaaaaa gaagaacata aacagcgccc aggttcggcg
     1261 tttcaatgca ttacgtgaag aatttacggt catagatcac gtcaaaataa gaagagggaa
     1321 gacaacatta gcaaggttgt gtacatttgt acacaattgc agacagagga aatgacatgc
     1381 gtcgcgctaa cgatccgcaa cggcgagaaa aaattatcca ggccacgctg gaggcggtga
     1441 aactttacgg aatacatgct gttacgcacc gcaaaattgc tacccttgcc ggggtaccgt
     1501 tgggatcgat gacctactat ttttcaggaa ttgatgagtt gttgctggag gcgttcagca
     1561 gttttactga gattatgtcc cggcaatatc aggcattttt tagcgatgtt agtgatgctc
     1621 aaggcgcatg ccaggctatc accgatatga tctacagctc acaggttgca acgccggata
     1681 acatggagct gatgtaccag ctctacgcgc tggctagccg aaaaccgcta ttaaaaacgg
     1741 taatgcaaaa ctggatgcag cgcagtcagc aaacgctcga acaatggttt gaacccggaa
     1801 ccgcccgcgc gcttgatgcg tttattgagg ggatgacgct gcattttgtc accgaccgta
     1861 agccgctatc gcgcgaggag attttgagga tggttgagag ggttgcaggg tagtagataa
     1921 attttagata acaaaaaacc catcaacctt gaaccgaaat ggcggggttg atgggctcca
     1981 caaaatgggg acatcaaaga aaagcagtgg cactaattaa gactgatgcc ctgcggaaaa
     2041 gttctgcggt tgtgcaaaaa aatttcattt tcagggcaac ttcagtttta tcctaatcct
     2101 ggccatacca tgacgatgat tgtccctgcc agcgtcagca ggacgttggc gattgcgtag
     2161 gtgcccgcat agcccagcgc agggatgtta ctgcgagctg tatcactgat gatctccatt
     2221 gccggcgcgc aggtgcgtgc gcccatcatt gcgccgaaca acagtgcgcg gttcattcgc
     2281 aatacataag caccgaacaa gaaacagata accacgggca caagactgac aattaatccg
     2341 gcaatcaaca tctgaccgcc aatcgcgccc aggccgttat taataccgct accggcgctc
     2401 agaccaacgc ctgccataaa caccatcaag ccgaactctt tcaccatgct taatgcacct
     2461 tgcggaatgt aaccgaaggt cgggtggtta gcacgcataa agcccagcat aattccggcg
     2521 aataacaacc cggcagcgtt ccccatgccg aaactgaatg tgctgaactg gaaggtgatc
     2581 atcccgatca tcagcccaat aacaaagaag gcgcaaaatg ccagcaggtc agtgacctgg
     2641 ctgtgaatcg agataaagcc gatgcgatcg gcgatggttt ttacgcgacg ggcatcaccg
     2701 ctgacttgta aaacgtcacc tttgttaagc acgacgttgt catctatcgg catctcaatc
     2761 tggctacgaa tgacgcggtt aaggaagcaa ccgtgatcgg tcaacttcag ttgtgcgaga
     2821 cgtttaccta cagcgttatg gtttttaacg accacttctt cagtgacgat acgcatgtcg
     2881 agaaggtcac gatcgaaaac ttctttaccg ttacggaagc tgggatcgag tcgggcatgg
     2941 gcgtcgggat agcctaccaa cgctatttca tcgcccattt gtagcacggc atcaccgtct
     3001 ggatttgcca gaatcccgtt acgtcgaata cgttcaatgt agcagccggt ttgtcgataa
     3061 atacccagtt cacgcagatt tttgccgtcg gtccaggcca ccagctccgg gccgacgcga
     3121 taggcgcgga tcaccggtaa ataaacctta cggttggcat cagtgtccag gccacgttcg
     3181 cgggcgattt gctgggcgct ggtctgtaag tcctgatgct gcaatttcgg caagtaacgc
     3241 gcaccaacaa tcaaactcac cagaccgatt aaataggtta aggcataccc gaggctcaga
     3301 ttatccagtg ccagtgagag ctgcctgctt tccatgccgg aatgacgcag tgtatcgcca
     3361 gcaccgacca gaaccggtgt cgacgtcata gagcctgcta acataccggc cgtcaggcca
     3421 atatcccagc caaacagctt acctaaccct aaggcgatca ccagcgcact gccaaccatc
     3481 accagtgcta acattaggta atttttccca tcgcgaaaaa aaatggaaaa aaagttcggt
     3541 ccggcttcga ccccgacgca gaaaataaac agcataaagc caagattaag cgcatcggtg
     3601 ttaatgctga aatgttgttg gcctaataac agcgatacga ctaaaacgcc aatggaatta
     3661 cccagttgga tcgaaccaag tcgtaacttt ccgagacata gcccaagcgc gaggaccaca
     3721 aataataaca gaatgtaatt cccatttaac aattcggcga cgtttatatt cacggaggct
     3781 aacttcttgt ttactagtaa gctgttgaaa gaaatggtaa tttacgataa tgttttttac
     3841 cagaattcag ggcgcagatt cattcagcgc acctaaacga tagtaaagta acaatatatt
     3901 ttactagtgt aatcacatta ggtatcaacg gctatatgaa ttgcgttggc ctatattagc
     3961 atggaatgcg aagcggcttt atcttactga acgccacact ggcgaaaaat gtgttcgata
     4021 gacgcagtgt caggaggaac gagtgaaaca taaacaacgt tgggcggggg caatctgctg
     4081 ttttgtcctc ttcattgtgg tgtgcctttt tctggcgacg cacatgaaag gcgcttttcg
     4141 ggctgccggg catcctgaaa tcggcttgct atttttcatt cttcctggag cagtcgccag
     4201 cttcttttca cagcgtagag aagtcctgaa acctctattt ggcgcaatgc tggcggcacc
     4261 ctgttcaatg ctcattatgc ggctgttttt ttcaccgacg cgctcattct ggcaagagct
     4321 ggcatggtta ctaagcgcgg tgttctggtg tgcgctgggg gcactgtgtt tcttatttat
     4381 cagtagtttg tttaaaccac agcacagaaa aaatcagtaa agccctcaac gcgagggctt
     4441 gtcagacgat caggcgtcca gattttcttt cacccatgca gcaaaatcgg tatagccgcc
     4501 gatatgttgc tgatcgacaa aaatctgcgg cacggtttct acgggtttac ctgccttttg
     4561 ttgtagatct tctttagtga tcccttccgc acgaatatct acatactgat actgaaaatc
     4621 atcgcgttca ttgctcaatt tctcagccag atcttttgca cgcacacagt aagggcaacc
     4681 cgaacgacca aaaataacgg tttgcattat ttctctcctc atagatttat gcctgtaatg
     4741 atcacgctaa aatgtattcg ctgaaagtag gtttaacctg ttgcattaat tgctaaaagc
     4801 tataactgtt aaacacaata cagtgaaaag ttttagactg aaggctcact ttgcagaggg
     4861 aagcgtatgc gcgcgatcgg taaattgcct aaaggcgtgt tgatactgga atttatcgga
     4921 atgatgctac tggcggtggc gctgctgtcg gtaagcgact ccctgtcgct gcctgagcca
     4981 ttttctcggc cagaagtgca gattctgatg atttttctcg gtgttttgct catgcttccc
     5041 gctgcggtgg tggttattct tcaggtggca aaacgtcttg ccccacagct gatgaaccgt
     5101 ccaccgcaat attcacgttc agaaagagaa aaagataatg acgccaacca ttgaacttat
     5161 ttgtggccat cgctccattc gccatttcac tgatgaaccc atttccgaag cgcagcgtga
     5221 ggcgattatt aacagcgccc gtgcgacgtc cagttccagt tttttgcagt gcagtagcat
     5281 tattcgcatt accgacaaag cgttacgtga agaactggtg acgctgaccg gcgggcaaaa
     5341 acacgtagcg caagcggcgg agttctgggt gttctgtgcc gactttaacc gccatttaca
     5401 gatctgtccg gatgctcagc tcggcctggc ggaacaactg ttgctcggtg tcgttgatac
     5461 ggcaatgatg gcgcagaatg cattaatcgc agcggaatcg ctgggattgg gcggggtata
     5521 tatcggcggc ctgcgcaata atattgaagc ggtgacgaaa ctgcttaaat taccgcagca
     5581 tgttctgccg ctgtttgggc tgtgccttgg ctggcctgcg gataatccgg atcttaagcc
     5641 gcgtttaccg gcctccattt tggtgcatga aaacagctat caaccgctgg ataaaggcgc
     5701 actggcgcag tatgacgagc aactggcgga atattacctc acccgtggca gcaataatcg
     5761 ccgggatacc tggagcgatc atatccgccg aacaatcatt aaagaaagcc gcccatttat
     5821 tctggattat ttgcacaaac agggttgggc gacgcgctaa aaccgccacg tcgatgtatg
     5881 atacgcgggc ttttgaccag gtctgacaga gaggtgcagg gtgaaaattg ccatattgtc
     5941 ccgggatgga acgctctatt cgtgtaagcg gctgcgtgaa gccgctatac agcgcggtca
     6001 cctggttgaa attcttgatc cgctttcttg ctacatgaac ataaatcctg cggcgtcttc
     6061 tattcactac aaaggccgca agttacccca ttttgacgca gtgatcccgc gtattggcac
     6121 cgccattacc ttttatggga cggcggcact gcgccagttc gagatgctgg ggagctatcc
     6181 gctcaatgag tcggtcgcca ttgcccgggc gcgtgacaaa ttgcgttcca tgcaactgct
     6241 ggcgcgtcag ggcatcgacc tgcctgtcac gggcattgcg cattcgccgg atgataccag
     6301 cgatttaatc gacatggtcg gtggtgcgcc gctggtggtc aagttggttg aaggcacgca
     6361 gggaattggc gtcgtgctgg cggagacgcg tcaggcggcg gaaagcgtga ttgacgcttt
     6421 ccgcggtctg aacgcgcata ttctggtgca ggaatatatc aaagaggcgc aagggtgcga
     6481 tatccgctgt ctggttgttg gcgatgaagt ggtcgctgcg attgaacggc gggcgaaaga
     6541 gggcgatttt cgttccaatt tgcatcgtgg cggcgcggca agcgtcgcca gtatcacacc
     6601 acaggagcgt gaaatcgcga taaaagccgc gcgaacgatg gcgctggacg ttgctggtgt
     6661 ggatattctg cgtgctaatc gcgggccgtt ggtgatggag gtgaatgcgt cgccggggct
     6721 ggaaggaata gaaaaaacca ccggtatcga catcgcgggt aaaatgatcc gctggatcga
     6781 acgccacgct acgacagaat attgcctgaa aacgggtggt tagtcgcaat cacattactg
     6841 atcatggttt tgcctgcgct ttttgcgtaa gctgtgccgg tctttttatc gaaagaggtt
     6901 gtacaaaatt atgacatcgc tggtcgttcc tggtctggat acgctgcgtc aatggctcga
     6961 tgacctgggg atgagttttt ttgaatgtga taactgtcag gctctgcatc tgccccatat
     7021 gcagaatttc gacggtgtct ttgatgccaa aatcgatctg atcgataaca cgatcctgtt
     7081 ttctgccatg gcggaagtcc gaccttcagc cgtattgccg ctggcggcgg atttatctgc
     7141 catcaatgcc agttcgctga ccgtgaaagc atttcttgat atgcaggatg ataatctgcc
     7201 aaagctggtg gtttgccagt ctttatccgt tatgcagggc gtaacctatg agcagtttgc
     7261 atggttcgtg cgtcagagcg aagagcagat ttcgatggtc attcttgaag ctaatgccca
     7321 tcaactgctg ttaccgactg acgatgaagg gcaaaacaac gttaccgaaa actatttcct
     7381 ccactgataa ctcctttcga gcacgcagtc gctggtgcag tggctgcgcg ctgcaaaatt
     7441 atctgctgtt tttaaccttt tcttaaagat tatttcactt ctcttgtgtc gatttggctt
     7501 tatcacatag agcaaatacg cataaaaatt tgttaaatac cgttttttaa tccgagctat
     7561 agtctcaaac cctggctaaa gttattcttg cgatgctttt atatagcgag cagtgctggc
     7621 cgggagagag ttctcttttc ttacaccccg ccgataaaaa atatgcacgt ttattgcata
     7681 tcttttagtg tgacaacttt tgttcgtttg ttaacgaact ttcagaagga aagagatatg
     7741 accgccttaa ataaaaaatg gctttcgggt ctggctgcgg gtgctctgat ggccgtctct
     7801 gtcggcacgc tcgcggctga acaaaaaaca ctccacgttt ataactggtc tgattatatc
     7861 gccccggaca cggtggccaa ttttgaaaaa gaaaccggta ttaaagtcgt ctacgatgtt
     7921 ttcgactcta acgaagtact ggaaggcaaa ttaatggcgg gtagcaccgg ctttgacttg
     7981 gtggttccat ctgccagctt tcttgaacgc cagttgactg cgggagtttt ccagccgctg
     8041 gacaaaagca aattgccgga gtggaagaat ctcgacccgg agctgctgaa gctggtcgcc
     8101 aaacacgatc ccgacaataa atttgctatg ccctatatgt gggcgacgac cgggattggc
     8161 tataacgttg ataaagttaa agctgtgctg ggcgaaaacg cgcccgtcga tagctgggac
     8221 ttgatcctca aacctgaaaa tctggaaaaa ctgaaaagct gcggtgtctc tttcctggat
     8281 gcgccagaag aagtttttgc taccgtgttg aattatctcg gcaaagatcc caacagcact
     8341 aaagcggatg attacaccgg accggcaacc gatctgctgt taaagctgcg cccgaacatt
     8401 cgttatttcc attcatctca atacattaac gacctggcaa acggcgatat ttgcgtcgct
     8461 atcggctggg caggtgatgt ctggcaggcg tcaaaccgcg cgaaggaagc gaagaatgga
     8521 gtgaatgtct cgttctcgat tccaaaagaa ggggcgatgg cgttctttga tgtattcgcc
     8581 atgcctgcgg atgccaaaaa caaagacgaa gcctatcagt tcctgaatta cctgctgcgc
     8641 ccggatgtag tagcgcatat ttccgacaat gtgttctatg ccaacgccaa taaagcagcc
     8701 acgccgctgg tgagtgcgga agtccgtgag aacccgggta tttatccgcc tgcggatgtt
     8761 cgtgcgaagc tgttcactct gaaagtgcag gacccgaaaa tcgaccgtgt gcgcacccgc
     8821 gcgtggacca aagtgaagag cggaaaataa tccgcagcct tagatgccgg gcgggcgcac
     8881 cacacccgcc ggcaattcgc accatcatgg tgcgcttgca cacattcaat gccggagagc
     8941 agccgtgaat gacgctatcc ctcgcccgca ggcgaaaacc cgtaaggcgc tgacgccgct
     9001 attagaaatc cgcaacctca ccaaatccta cgatggtcaa catgcggtgg atgatgtcag
     9061 cctgaccatc tacaaaggtg aaatcttcgc gctgctgggc gcatccggct gtggcaagtc
     9121 cacgctgctg cgtatgctgg caggtttcga acaaccttct gccggacaga taatgcttga
     9181 tggcatcgat ttgtcacagg tgccgcctta cctgcgcccc atcaatatga tgtttcagtc
     9241 ttacgcgctg tttccccata tgactgtgga gcagaacatc gcctttggcc tgaaacagga
     9301 caaactaccg aaagcggaaa ttgccacccg ggtcaacgag atgcttgggc tggtgcacat
     9361 gcaggagttc gccaaacgca aaccgcatca gctttccggt ggtcagcgac agcgtgtggc
     9421 cctggcccga agccttgcca aacgcccgaa actattactg ctcgatgagc cgatgggcgc
     9481 gctggataaa aagctgcgtg accggatgca gcttgaagtg gtggatattc tggagcgcgt
     9541 cggtgtgact tgtgtgatgg tcacccacga tcaggaagag gcgatgacca tggcggggcg
     9601 tatcgccatt atgaatcgcg ggaaatttgt ccagattggc gagccggaag agatctacga
     9661 gcatccaact acccgctaca gcgctgaatt tatcggttcg gttaatgtct ttgaaggtgt
     9721 actcaaagag cgtcaggaag atggcctggt gcttgattcg ccggggctgg tgcatccact
     9781 gaaagtcgat gcggatgctt cagtggtcga taacgtgccg gtacacgtag cgctgcgccc
     9841 ggaaaaaatc atgctttgcg aagagccgcc cgccaatggt tgtaacttcg cggtggggga
     9901 ggtgatacac attgcctatc tcggcgatct ttcggtgtat cacgttcgtc tgaaaagtgg
     9961 gcagatgatt agcgcccagc tacaaaacgc ccatcgtcat cgtaaagggt taccgacctg
    10021 gggcgacgaa gtgcgtttgt gctgggaagt ggacagctgt gtggtgctga cggtttaagg
    10081 agcaaagatg aatacacttg aacctgctgc ccagtcgaaa ccgccgggcg gatttaagct
    10141 gtggttgtcg cagctgcaaa tgaagcatgg gcgcaaactg gtcattgcgt tgccatatat
    10201 ctggttgatc ttgctgtttc tgctgccatt tctgattgtc tttaaaataa gcctggcgga
    10261 gatggcgcgc gctattccac cttataccga actgatggag tgggctgacg ggcaactgtc
    10321 catcactctt aatctcggta attttctgca actgaccgac gatccgctct atttcgatgc
    10381 ttatctccag tcgctacagg tggctgcgat ttcgacattt tgctgtttac tgatcggcta
    10441 tccgctggcg tgggcggtgg cgcacagtaa gccttcgacc cgtaatattt tattactact
    10501 ggtgatcctg ccgtcgtgga cctcgtttct gatccgcgtt tatgcctgga tgggaatatt
    10561 aaaaaacaac ggtgtgctga ataattttct gctgtggctg ggggttatcg atcaaccgct
    10621 gaccattctt cataccaatc tggcagttta tattggcatt gtttacgcct acgtgccgtt
    10681 tatggtactg ccgatttata ccgcgttgat tcgtattgat tattcgctgg tggaagcagc
    10741 gctggatctc ggtgcacgac cgctgaaaac gttctttact gtgatcgtgc cgctgactaa
    10801 aggtgggatt attgccggat cgatgctggt gtttatcccg gctgtgggcg agtttgtgat
    10861 cccggaactg ctcggtggcc cggacagcat catgatcggg cgcgtgctat ggcaagagtt
    10921 tttcaataac cgcgactggc cggtggcctc ggcggtagcg atcatcatgt tgctgctgct
    10981 aattgtgccg ataatgtggt tccacaaaca ccagcaaaaa agcgtgggag aacacggatg
    11041 aataatttac cggtagttcg ttcgccctgg cggattgtga ttttgctgct gggctttacc
    11101 tttctctacg cgccaatgct gatgctggtt atctattcgt ttaacagctc gaagctggtg
    11161 acggtgtggg ccggctggtc aacgcgctgg tatggtgagt tattgcgcga tgacgcgatg
    11221 atgagtgcgg ttggtttaag cctgacaatt gctgcctgtg cggcaacggc ggcggcgatc
    11281 ctcgggacta ttgcggcggt ggtgctggtg cgctttggca ggtttcgcgg atcaaatggc
    11341 tttgccttta tgatcaccgc gccgctggtg atgccagatg tcatcacggg cttgtcgctg
    11401 ttgttattat tcgtcgcgct tgctcatgcc attggctggc ctgcggaccg cggtatgctc
    11461 accatctggc tggcgcatgt cactttttgt acggcttatg tggcggtcgt tatttcttca
    11521 agattgcggg aactggatcg ctcgatagaa gaagcagcga tggatctcgg tgcgacgccg
    11581 ctgaaagtgt ttttcgtcat tacgctaccg atgatcatgc ccgcgatcat ttctggctgg
    11641 ttactggctt ttactttgtc gcttgatgat ctggtgatcg ccagctttgt ttccgggccg
    11701 ggagccacca cgttaccgat gctggtcttt tccagcgtgc ggatgggggt gaatccggaa
    11761 atcaacgccc ttgcaacgtt aattctcggt gcggtcggaa ttgtcggatt tatcgcctgg
    11821 tatctgatgg ctcgcgcaga aaaacagcgg atacgcgata tccagcgtgc aagacatggc
    11881 tgaagacact aaaatttgcc aacctggcta cataatgccg cgcatgccgc ggcattgttt
    11941 tcatggaaga cgaaacgttg ggatttttta agaaaacatc ttcatctcat gctcgcctga
    12001 atgtgcctgc gctggtgcag gtggcggcgc tcgccattat tatgatccgt ggcctcgacg
    12061 tgctgatgat tttcaatacg ctgggcgtgc gcggtattgg cgagttcatt catcgcagcg
    12121 tacaaacctg gagtttaacg ctggtctttt taagcagtct ggtgctggtt ttcattgaga
    12181 tctggtgtgc gttttcactg gtgaaagggc gtcgctgggc gcgctggcta tatctgctga
    12241 cacaaatcac cgccgcaagt tacttgtggg cggcttcgct ggggtacggt tatccggagc
    12301 tgttcagcat tcccggtgaa tcaaaacgtg aaatcttcca tagcctgatg ctgcagaagc
    12361 tgccggatat gctcatcctg atgctgctgt tcgttccctc gaccagtcgg cggttcttcc
    12421 agttgcaata atgtgtataa tcgtcgcccc tgatgatgtg aaggtcaatg tatgcagtgc
    12481 gcactttacg acgcgggtcg ctgtcgttcc tgtcagtgga taacgcagcc gattccagag
    12541 caactctccg ctaaaaccgc cgatcttaaa aatctgctcg ccgattttcc ggttgaggaa
    12601 tggtgcgcgc cggtgtcagg cccggaacaa gggtttcgta ataaagccaa aatggtggtg
    12661 agtggtagcg ttgaaaaacc actgctcggt atgctgcatc gcgacggcac accagaagac
    12721 ctttgtgact gcccgcttta tcctgcctca tttgcgcccg tttttgcggc gctaaaaccg
    12781 tttatcgccc gtgcggggct gacaccctac aacgtggcgc gtaagcgtgg cgaactgaaa
    12841 tacattctgc tgactgaaag ccagagcgat ggcggcatga tgctgcgctt tgtactgcgt
    12901 tctgaaacca aactggcaca actgcgtaag gcgctgccgt ggttacagga acaactaccg
    12961 caactgaaag ttattaccgt caatattcag ccggtacata tggcgattat ggaaggggag
    13021 acggagatct accttactga acaacaggcg ctggcagagc gttttaatga cgtaccgctg
    13081 tggatccgtc cgcaaagttt cttccagact aatccggcgg tcgccagcca gctgtacgct
    13141 accgcgcgcg actgggtacg gcagctgcca gttaaacata tgtgggatct gttctgcggt
    13201 gtgggtggct ttggtttaca ctgcgcgacg cctgacatgc agttaaccgg gatcgaaatt
    13261 gcatcagagg ccattgcctg tgcaaagcag tcagccgctg aactgggctt aacgcgtttg
    13321 caatttcagg cgctggactc cactcagttt gccaccgctc agggggaggt gccggagctg
    13381 gtgctggtta atccgccgcg ccgcggcatt ggtaaaccgc tatgtgatta tctctcaaca
    13441 atggcaccgc gttttattat ctactccagc tgtaacgccc aaaccatggc gaaagatatc
    13501 cgcgaactgc caggttaccg catcgaacgg gtacagcttt ttgatatgtt cccgcacacc
    13561 gcgcactatg aagtgctgac gctgctggtg aagcaataaa aaagccgcag gtgcggcttc
    13621 agattgctga tcaggtgcgc tttgttcatg ccggatgcgg cgtgtacgcc ttatccggcc
    13681 tacaaaatca tgctaaatca atacattgca gagttgatgt gggccggata agcgcagcgc
    13741 atcaggcaat cttacttttg tcatcagtct caattactgc ggaaaccact ggtcattgat
    13801 tttctgatat gtaccgtcag ctttaattgc tgccagcgcg ttattcagtt tttccagcag
    13861 agctttgtta tccggacgta cagcgatgcc cagaccggtg ccgaagtatt gcggatcggt
    13921 cactttttca gtagcaacgc ccagttgcgg attggttttc agccattcgt ttaccaccgc
    13981 tgtgtcacca aataccccat caatacgacc atttttcaga tcgataaagg cattctgata
    14041 actgtcatag gagacagttt tcacttcggg gtgctgatcc tgaatatatt tctggtgcgt
    14101 ggtgccgttt tccatcccaa tacgtttgcc tttcagatcg gcaaacgttt tgtaggtatc
    14161 ttttttggca atcacgacgg ctgagttttc atagtagggc gtggtaaacg atacctgctt
    14221 gctacgctcg ggcgtgatat ccataccgga gattacggcg tcatattttc tgaatttcag
    14281 tgacgggatc aggctgtcga acgcgtgatt agtaaaagta cattctgcct gcatttgttt
    14341 gcacaaggct tttgccagat cgatatcaaa gccgacaatc tcattattag cacctataga
    14401 ttcaaagggt ggataggtgg ctgaaacgcc aaaattgatt ttctctgcgg cagaagcacc
    14461 gaaagtaaag gaagcaagta aagcggcaag aactaacttt ttcatgatgg aactcccgtc
    14521 tgtcaatctt atgatttttg gccgtgtctg cggcatggga taacaatgcc atcaagtgaa
    14581 tttatatgca ataaatatga ttaaataatt taaatgaaat aaaaaaagac ggatacctta
    14641 gtgagttgtc cgtcttcatt ataagaattt atgcactatg taggccggat aaggcgtcca
    14701 cgccgcatcc ggcacaggca ccgtgctgat gtctgatgcg acgctggcgc gtcttatcag
    14761 acctacaaac ccccccggcg aatgtacgca gctacattaa tttcgccgtt cgaatgccag
    14821 tgctttgcgc tcgatcagac gcatcatcag cgtcagcagg ccgttgacga ccaggtaaat
    14881 aatccctgcc gcaccgaaca ccattacatc gtaggtgcgt ccgtacaaca actggctgta
    14941 tcccatcact tccatcagcg taatggtgta agccagagag gtacttttga acaccagcac
    15001 cacttcgttg gaataagaag agagcgagcg tttaaaggca tacggcagca ggatcgccag
    15061 cgtatctttt ttgctcattc ccagggcgct acaggactgc cactgacctt ctgggatcgc
    15121 acgaattgca ccgtaaaaca gctgcgtggt atacgccgca ctgttcagcg ataacgcaat
    15181 cagcgcacat aaccacggtt ctgacaacaa atgccacagt gccggatact cctgcaaagt
    15241 cggaaactgg cccggcccgt aataaatcag gaagatctgc accagcagcg gcgtaccggt
    15301 aaacagcgtg atataacccc gcaccagcca caccagcacc ggcgttttca gcgtcaggat
    15361 gatggtaaaa atcaatgcca gaatcagtgc cacaatcagc gaggcaacgg ttagcgtcag
    15421 gctggtgtgt agccctttca tcagttcggg taaatactca aacattagct gggcctccgc
    15481 tcaaaacgtg tcgcgcgcag gtcaatgcgt ttgagaatgt actgactgag cagggtgatc
    15541 accaggtaaa tcgccgccgc cacaatgtac caggtaaatg gttcctgggt acgagtagcg
    15601 atgctttttg tttgcagcat taaatcattc acactaatca aactgaccag cgcggtatct
    15661 ttcagcagca ccagccactg gttaccgagg ccaggcagcg catgacgcca catctgcggc
    15721 atcaccagac ggaaaaagat agccgatttc gacagcccca gcgcctgacc agactcccac
    15781 tgacccaccg gcaccgcttt caacgcgccc cgaagcgttt gcgaggcata ggcggcatac
    15841 agcagtgaca gagcgatgac accacaaagg aacgggctaa cgtcgaagtt ctcaatgtcc
    15901 atctgcactg ggatctgcac gaacccaaga ttgatagtga agccatccga aagcgtcagc
    15961 agcagctgcg aggagccaaa atagataaac agcaccacca gaatttctgg caggccacgc
    16021 agaatggtta ccagcgctga acctgcccac gcgacaggac gccattttgc cgactcccat
    16081 accgcaaaga acatcgccag cgccagcccg acaatcaatg cacaaacggc aaggccgacg
    16141 gtcatcccgg cggcgcttgc taaaggaaaa aattcattca tcaggaatta cttctggaac
    16201 catttgttgt agatggtttc gtaagtgcca tctttcttca ctttttccag cgcagtgttg
    16261 agtttctgct gcagctcagt gttgccctga cgtaccgcga tgccgaggcc agtgccgaag
    16321 taatctttat cggtcacttt gtcgcccacc gccgccagtt tcgggttatc tttcagccac
    16381 tcagtgacca ctgcggtgtc accgaagacg ccgtcgatac gcccgttttg cagatccagt
    16441 tttgcgttct ggtagctgtc atacggaacg gtagtgattt ccgggtgctt atccataatg
    16501 aatttctggt gtgtcgtccc gttctgtacg ccgacttttt tgcctttcag ctgatcaaca
    16561 ctggtgtatt tgccttgctg acccacaaac agggcagagt tgtcatagta cggggtggta
    16621 aacagcacct gcttttcacg ctccggagtg atatccatgc ccgccatcac ggcttctacg
    16681 cgacggaatt tcaggcttgg gatcaggctg tcaaacgcct ggttagagaa agtgcaggtt
    16741 gcatcaatct ctttacacag cgcttgtgcc aggtcgacgt caaaaccaac gatctggttg
    16801 tttgcatcaa tcgattcaaa cggaggatag gatgcttcgg tagcaaaacg aatggtttcg
    16861 gcagctgtgg cggaaagact aaaacctgca attaacgcgg caatcagaac ttttttcatt
    16921 gttgttatcc cgaatcttag tgagagagat agtttttaaa tgcttcggtt tgcggctcgg
    16981 taaagcagct cgcgtcgcct tgttctacga tatgaccatt ttccatatac accactcggc
    17041 tggcggtttt acgcgccact tcaacttcgt gggtgacgat cacctgggta atattcgttt
    17101 ctgccagctc acgaatgatg ctgacgattt gtgccgtaat ttccgggtcc agtgcggcgg
    17161 tcggttcatc gaacagcagt acctgcggtt ccatcatcaa cgcacgggca atagcaacac
    17221 gctgctgctg accaccagaa agatgcagcg ggtaacgatc gctataaggt ttgagacgca
    17281 gacgttccag cagtttttct gcacgggcca gcgcctgatc tttactcaac cccagtacac
    17341 ggcagggcgc ttcaatcagg ttttgctgca cggtcagatg cggccacagg ttgtattgct
    17401 gaaacaccat gccaacgtta cgacgcaaat cgcgaatcgc tttgtcagag ggtgttttgg
    17461 tgaaatcgaa atggttgcct gcaatgttga gcgtaccgga gcgcggcatc tcaagcagat
    17521 tgagtacacg cagcagcgag cttttacccg cgccgctggg gccaagtaac accagcgttt
    17581 cgccctgtgg gcaatccagc gtgatatcga acagcgcctg atgcgcgccg tagaagcaat
    17641 taatgccgtt taattgaata ctcattgaca ctcgtatact ggcagtctga tagctattga
    17701 ggtcgaagat agtacctttg acagaataat tatgcaatat ttctgcttta aaagttaaaa
    17761 gcaaagcgca ttattcaata aacatagcac aaaataacgg gggcggtggt cggcgagcat
    17821 aaatgtcggc attcctcacg aaatgccgga caatttacgg ggtttattgg ttgatcaagg
    17881 cgttagcgat tctcgatgga ctgacggagc gtacccgccg tggcatgaac gctgccgcct
    17941 aaatagcgta catcgtcgat cacccaacac tggccttcct gaatcattaa cacttcatcc
    18001 tgccagctct gatcaccctg ttttagatcc acacgcagag gaatatttcg cgcatcacga
    18061 ttcgggattg ttgaggcact ggcaacacgg gcgctctctg gcaaggttgt gcgactggag
    18121 aatggatcgt tggtcagtag ttcccgatgg ttattatccc gggaggcatc gctaagcagt
    18181 gtcgccagtt tgtcgctcag ataagggcgc aaggcggtga tgtcgttgct gcggtgcaaa
    18241 atgcggtagt cataaaattg ctgggccacg ttatccgggc ctccttcaac gcaaggacca
    18301 ctgcgtgtgc cgttatcttt ataagctgga gtgactgtgg tgcaggcact gaggagcagt
    18361 gcgcagggga taagcattgt caatttgctg tagcgcataa tgatttcctt ataagcgatc
    18421 gctctgaaag cgttctacga taataatgat atcctttcaa taatagcgta tcagtctgat
    18481 aatgctttag agatcgaagg cttaacaaac aaggagatcg atcatgcaat tttctacaac
    18541 cccaactctg gaaggccaga tcatcgttga atattgcggt gtggtgaccg gcgaagcgat
    18601 tttaggtgcc aatattttcc gtgatttctt tgccggtatc cgcgatatcg ttggcggacg
    18661 ttccggtgcg tatgaaaaag aactgcgtaa agcacgggag atcgcctttg aggaattagg
    18721 ctcccaggcg cgggcgctgg gggccgatgc cgtcgtcggt attgatatcg actacgaaac
    18781 ggtcgggcaa aacggcagta tgctgatggt tagcgtcagc ggtacggcgg tgaaaacgcg
    18841 tcgatgagaa gagtcttctg gctgatcgct gccgctctgt tactggcagg gtgtgcaggc
    18901 gaaaaaggca ttgtcgagaa agagggatat cagcttgata cccgacgcca ggcgcaggcg
    18961 gcgtatccgc gcattaaagt gctggtgatc cactacaccg cagatgattt tgatagctcg
    19021 ctggcgacac tgaccgataa gcaggtcagc tcgcattatc tggtccctgc ggtaccaccg
    19081 cgatacaacg gtaaaccgcg catctggcaa ctggtgccgg aacaagaact ggcctggcat
    19141 gcggggatta gcgcctggcg cggggcaacg cgccttaacg acacctctat tggcattgag
    19201 ctggaaaacc gtggctggca aaaatcggcc ggagtgaaat attttgcccc gtttgaaccg
    19261 gcacagattc aggcgcttat tccactggcg aaagatatta ttgcccgtta tcacatcaag
    19321 ccggaaaacg tagtggcaca tgcggatatc gcaccgcagc gcaaagacga tccggggcca
    19381 ttatttccct ggcagcaact ggcgcagcag gggattggtg cctggccgga tgcgcagcgg
    19441 gttaactttt accttgccgg gcgcgcgccg cacacaccgg tagataccgc gtcattgctg
    19501 gagcttttgg cgcgctacgg ttatgacgtt aaacctgata tgacaccgcg cgagcagcgg
    19561 cgcgtgatta tggcattcca gatgcatttc cgcccgacgt tatataacgg cgaagcggat
    19621 gcagaaactc aggcgattgc cgaagcattg ctggagaaat acgggcagga ttagcgcggc
    19681 agttttccgt ggtcgcgtag ccaggcggca gttttctcga taccttcatc cagggtgatg
    19741 accggctgat aacctaactc ttcctgcgca cgcgtaatat ccagcgtaaa gtcaaaattc
    19801 aacttggaga cgccgtagtg ggtcagcggc ggctcttttg ctgacttgcg gcctaaacgc
    19861 tccatgctgc gggcgatcat atccagcatc gggtagggga cggaacgaat acgacagtca
    19921 atattcaact cgtcgatcag cttctgcacg atgctgcgca gtgtgcgatg ctcgccgttg
    19981 gtgatgttgt acacacgccc ggaaggtagc ttatcgcagg cttcctggct tgccagccac
    20041 attgcgtgca cggcattttc atagtaggtc atatcgacca gcgcactgcc gccatgcggt
    20101 aacagaatac tgccgtagtg gtgcatcata tgcgccagac ggggaataaa gactttatcg
    20161 tgcggtccga acagactttg tgggcgcaga atagtaaagc gcgtttgtgg attcgcctgc
    20221 gaaagcatat tgatcacttc ttcgctggct gctttgctgc gggcaaactc gttggcgaag
    20281 cggtgagggc gaaaatcttc tttaatatcg cgatggtggt gataatcgaa gtacagggag
    20341 ggggaagaga tatgaataaa gttacgcaca ccccaggcga cagtccattc acccaggcgg
    20401 cgagtggcgc gaacgttagc cagatcgaaa gcctgttgtg tcccccaggg tgaggtaaag
    20461 ctggagcagt gccacagcgt atcaatgccc gcgagcatca ctttagcttg tgatgaaacc
    20521 agctcggtca gatccgccgg aacaaactct gcgcccattt tttccagcaa tttgcccatt
    20581 gcctcgttgc gaccggtcgc tcgcacgctg atgcctttct ggcataaaaa ctctaccgcg
    20641 tttcgaccta agccgctggt ggcgccggta accagtacct tcatatcaat ccactgttgt
    20701 tgagaaaata acgtgcgcat tcttccgtga tttcccccat gatgcaatgg gaaacatgaa
    20761 agaataacgc aggttttgtc gattaatctg tgctttgttc tgccagtctg gcgatttgtt
    20821 ttgccattcc gcgaaaaata aacagatgcg cggggatcat caataaccag taaaacagcc
    20881 ccggcatacc gtgcggatgc cagaaagcgc ggacatcgat agtactatag tcgcctttat
    20941 cttccaggct aaaacacagt cgtcccagcc ccggcgcttt catgccaaat aacaacgtaa
    21001 gttgtttttc cggttcaacg acaatcactt tccagctatc caccgcatcg ccagtctgta
    21061 aatattcgcg ctccgggcgg cctttcgcca gcttatgacc gatcgcgcgg tccatcaacg
    21121 cccgtgtctg ccacaaaata ttgccaaaga aataacgctc tttaccgccg atttggttca
    21181 ctacctgcca taaagcagca aggctggcgg acgttttaac ggtaaacccc gcctgtttgg
    21241 caaaataacc gtactctggt cgccagcggg caaaggcctg agcgtcgtag ccccagtcgc
    21301 tggagttgac cagtttttcc tcctctttca acgtgctacg taccgcgtca tcgaaagcga
    21361 tcagccgttg tgggatgagt gcacgtagcg cggtatcatc cgccagcaga tcgtgtttca
    21421 gcccctgaat caacgccctg gcggtggtgg gcggtacgga agtaatcaca ttgagaa
//
LOCUS       DAZFWL020000079        21113 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFWL020000079 DAZFWL020000000
VERSION     DAZFWL020000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.079"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..247)
                     /locus_tag="ACHKNP_RS15330"
                     /old_locus_tag="pgaptmp_003066"
     CDS             complement(<1..247)
                     /locus_tag="ACHKNP_RS15330"
                     /old_locus_tag="pgaptmp_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGU4705192.1"
                     /translation="MRDNGLNNMFFSLCQINDNHSFTSSSHTKKTKSYNYSKHHKNTL
                     IDNKALSLFKMDDHEKVIGLIQKMKRIYDSLPSGKITK"
     gene            complement(425..2074)
                     /gene="actP"
                     /locus_tag="ACHKNP_RS15335"
                     /old_locus_tag="pgaptmp_003067"
     CDS             complement(425..2074)
                     /gene="actP"
                     /locus_tag="ACHKNP_RS15335"
                     /old_locus_tag="pgaptmp_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HGU4705193.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(2071..2385)
                     /gene="yjcH"
                     /locus_tag="ACHKNP_RS15340"
                     /old_locus_tag="pgaptmp_003068"
     CDS             complement(2071..2385)
                     /gene="yjcH"
                     /locus_tag="ACHKNP_RS15340"
                     /old_locus_tag="pgaptmp_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HGU4705194.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2586..4544)
                     /gene="acs"
                     /locus_tag="ACHKNP_RS15345"
                     /old_locus_tag="pgaptmp_003069"
     CDS             complement(2586..4544)
                     /gene="acs"
                     /locus_tag="ACHKNP_RS15345"
                     /old_locus_tag="pgaptmp_003069"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HGU4705195.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4936..6372
                     /gene="nrfA"
                     /locus_tag="ACHKNP_RS15350"
                     /old_locus_tag="pgaptmp_003070"
     CDS             4936..6372
                     /gene="nrfA"
                     /locus_tag="ACHKNP_RS15350"
                     /old_locus_tag="pgaptmp_003070"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HGU4705196.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6417..6983
                     /gene="nrfB"
                     /locus_tag="ACHKNP_RS15355"
                     /old_locus_tag="pgaptmp_003071"
     CDS             6417..6983
                     /gene="nrfB"
                     /locus_tag="ACHKNP_RS15355"
                     /old_locus_tag="pgaptmp_003071"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HGU4705197.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6980..7651
                     /gene="nrfC"
                     /locus_tag="ACHKNP_RS15360"
                     /old_locus_tag="pgaptmp_003072"
     CDS             6980..7651
                     /gene="nrfC"
                     /locus_tag="ACHKNP_RS15360"
                     /old_locus_tag="pgaptmp_003072"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HGU4705198.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7648..8604
                     /gene="nrfD"
                     /locus_tag="ACHKNP_RS15365"
                     /old_locus_tag="pgaptmp_003073"
     CDS             7648..8604
                     /gene="nrfD"
                     /locus_tag="ACHKNP_RS15365"
                     /old_locus_tag="pgaptmp_003073"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HGU4705199.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8624..10342
                     /gene="nrfE"
                     /locus_tag="ACHKNP_RS15370"
                     /old_locus_tag="pgaptmp_003074"
     CDS             8624..10342
                     /gene="nrfE"
                     /locus_tag="ACHKNP_RS15370"
                     /old_locus_tag="pgaptmp_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HGU4705200.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10335..10718
                     /gene="nrfF"
                     /locus_tag="ACHKNP_RS15375"
                     /old_locus_tag="pgaptmp_003075"
     CDS             10335..10718
                     /gene="nrfF"
                     /locus_tag="ACHKNP_RS15375"
                     /old_locus_tag="pgaptmp_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HGU4705201.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10715..11311
                     /gene="nrfG"
                     /locus_tag="ACHKNP_RS15380"
                     /old_locus_tag="pgaptmp_003076"
     CDS             10715..11311
                     /gene="nrfG"
                     /locus_tag="ACHKNP_RS15380"
                     /old_locus_tag="pgaptmp_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HGU4705202.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11653..12966
                     /gene="gltP"
                     /locus_tag="ACHKNP_RS15385"
                     /old_locus_tag="pgaptmp_003077"
     CDS             11653..12966
                     /gene="gltP"
                     /locus_tag="ACHKNP_RS15385"
                     /old_locus_tag="pgaptmp_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HGU4705203.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(13044..13733)
                     /gene="yjcO"
                     /locus_tag="ACHKNP_RS15390"
                     /old_locus_tag="pgaptmp_003078"
     CDS             complement(13044..13733)
                     /gene="yjcO"
                     /locus_tag="ACHKNP_RS15390"
                     /old_locus_tag="pgaptmp_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HGU4705204.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13827..15974)
                     /gene="fdhF"
                     /locus_tag="ACHKNP_RS15395"
                     /old_locus_tag="pgaptmp_003079"
     CDS             complement(13827..15974)
                     /gene="fdhF"
                     /locus_tag="ACHKNP_RS15395"
                     /old_locus_tag="pgaptmp_003079"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15555..15557),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HGU4705205.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(16172..17638)
                     /gene="mdtP"
                     /locus_tag="ACHKNP_RS15400"
                     /old_locus_tag="pgaptmp_003080"
     CDS             complement(16172..17638)
                     /gene="mdtP"
                     /locus_tag="ACHKNP_RS15400"
                     /old_locus_tag="pgaptmp_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HGU4705206.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALLQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVALFAGLDLDLWGV
                     HRSAVAAAIGAHNATLAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFQGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17635..19686)
                     /gene="mdtO"
                     /locus_tag="ACHKNP_RS15405"
                     /old_locus_tag="pgaptmp_003081"
     CDS             complement(17635..19686)
                     /gene="mdtO"
                     /locus_tag="ACHKNP_RS15405"
                     /old_locus_tag="pgaptmp_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HGU4705207.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLFIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19686..20717)
                     /gene="mdtN"
                     /locus_tag="ACHKNP_RS15410"
                     /old_locus_tag="pgaptmp_003082"
     CDS             complement(19686..20717)
                     /gene="mdtN"
                     /locus_tag="ACHKNP_RS15410"
                     /old_locus_tag="pgaptmp_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HGU4705208.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20736..21011)
                     /gene="ytcA"
                     /locus_tag="ACHKNP_RS15415"
                     /old_locus_tag="pgaptmp_003083"
     CDS             complement(20736..21011)
                     /gene="ytcA"
                     /locus_tag="ACHKNP_RS15415"
                     /old_locus_tag="pgaptmp_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HGU4705209.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4787 a         5557 c         5748 g         5021 t
ORIGIN      
        1 ccttcgtgat ttttcctgat ggtaaactat cataaattct tttcattttc tgaatcaaac
       61 ctatcacttt ttcatgatca tccatcttga aaagagagag ggctttattg tcaattaacg
      121 tgtttttatg atgtttgctg taattatatg attttgtttt ctttgtatgt gatgaacttg
      181 tgaaagagtg gttatcgtta atctggcaaa gagagaaaaa catattattt aaaccattat
      241 cgcgcataaa atatccttat aattaacaag aaagaaaagg catattctct gcattatcat
      301 tttctacgac tgtcaaaaat cgctcatttt ttaatgagtt tatttgttta atattatggg
      361 aaaaggtgat gcatgctgga gaggaagagt attccccggt caggcgaccg gggaagggga
      421 gagattaatg tgcgcggcct tgctcaacgc caaagccggt ctgggagcgg ataaactgtg
      481 cacggaacag ttcacgctcg cgcgcacctt ccgctgagtt atcggttgcc gagaagaacc
      541 agatgccgag gaatgccacg ctgatagaga acagcgccgg gtattcatac gggaagatgg
      601 ctttttcgtg accaaggatc tgtacccaaa tcgtcgggcc gaggatcatc agtaccactg
      661 cggtaatcag ccccagccag ccacccatca tcgcgccacg agtggtcagt ttcgaccagt
      721 acatagaaag cagaatgatc gggaagttac agctcgctgc gatggcaaac gccaggccta
      781 ccatgaaggc gatgttctga ttctcaaaca gcacgccgag gataatggca atcacaccta
      841 aaatcagcac ggtgattttc gatacccgca gttcttcacg ttcggtcgcg ccttttttga
      901 agacgttagc gtacaggtca tgcgaaaccg ctgatgcgcc cgccagcgtc agaccagcaa
      961 ccaccgcaag aatggtggca aaagcaaccg ctgaaataaa accgaggaac aggttgccgc
     1021 ccactgcatt cgccaggtga acggccgcca tgttgttacc accaatcaga tggcccgccg
     1081 cgtctttata ttccggattc gcaccaacca gcatgatcgc gccgaagccg ataataaagg
     1141 tcagaatata gaagtagccc ataaacccgg tggcgtagaa cacgctctta cgtgcttcgc
     1201 gggcatcgct gactgtaaag aagcgcatca gaatgtgcgg caagcccgcc gtaccaaaca
     1261 tcagtcccag acccagagag agcgcggaga tcggatcttt caccagcccg cccggcttca
     1321 tgatgtcgac acctttcggg tgtaccgcca tcgcttcact gaacagattg ttgaagctaa
     1381 agccgacgtg tttcatcacc ataaaggcca taaagctggc accgaacagc aacagcacgg
     1441 ctttgataat ctgtacccaa gtggtcgcca gcatgccgcc gaacaggacg tacatcatca
     1501 tcagcacgcc gaccagcacc accgcaatgt gatagttaag gccaaacagc agctcgatca
     1561 gtttacctgc gcccaccatc tgggcgataa ggtaaagcgc caccaccacc agagaaccac
     1621 aggccgaaag aatacgaatc ggcccttgtt tcagacggta agaggccaca tcggcaaagg
     1681 tgtagcgccc caggttacgc agacgttcgg caatcaggaa caaaatgatc ggccagccca
     1741 ccaggaagcc cagcgagtaa attaagccgt catagccgga ggtaaacacc agcgcggaga
     1801 tccccaagaa tgaggcggcg gacatatagt ccccggcaat cgccagcccg ttctggaagc
     1861 cagtgatatt gccgcctgcg gtgtagtagt cgctacgaga acgtacgcgt tttgatgccc
     1921 agtaggtaat gccgagcgta aacacgacga aaatcaggaa cataataatc gcctgccagt
     1981 tcgttggctg gcgctctacg gccccgctaa tagcatccgc ggcgttagct gcgaaaggga
     2041 gtgtggcggc aagcgccgtc agaactcttt tcatgatgct tgtacctcat gcaggacttc
     2101 attattaaga cggtcgaatt cgccgttcgc ccgccagatg tagataccgg tgagaacaaa
     2161 ggagatcaca atcactccaa caccaattgg aataccgcgt gtgacgctgg tgttcggatt
     2221 cagcggcgtg cccagccagc cgggcgcgaa ggcgatcagt aaaataaagc cgatataaac
     2281 tgccagcata ataatcgaca ggatggtggc aaaccgttgc cgtttttcga ctaactccct
     2341 gaaatgcgca ttgtcttcta tccgctgata aatcgtacca ttcatcacag attctccaga
     2401 ggtaatgtag ggattgtttt aattcccgct cccttatggg agaaggttaa cgctcgggta
     2461 acccttgccg aatgtaggcc ggataaggcg ttatacgccg catccggcaa tcaatgcctg
     2521 atgcgacgct gtcgcgtctt atcaggccta caaaccgtta ccgactcgca tccggcaatt
     2581 gtgggttacg atggcatcgc gatagcctgc ttctcttcaa gcagcttctc gactacgcca
     2641 ggatcggcaa gcgtcgaggt atcgcccagg ttgctggtat cgcccgccgc aattttgcgc
     2701 agaatacggc gcataatttt gccggagcgg gttttaggca gggagtcggt ccagtgcagc
     2761 acgtctggcg tcgccagcgg gccaatctct ttacgcaccc agttgcggac ttctgcgtac
     2821 agttctggtg acggttcctc cccgtgatta agcgtgacgt aggcgtagat cgcctgacct
     2881 ttaatattgt gcggaatacc tactacggcg gcttcggcaa tcttcggatg cgccaccagc
     2941 gccgactcaa tctctgccgt ccccagacgg tgaccggaga cgttcagcac gtcgtccaca
     3001 cgcccggtga tccagtaata gccatcttca tcgcgacgcg cgccgtcgcc gctgaaatac
     3061 atatttttga aggtggagaa gtaggtctgt tcaaaacgtt cgtgatcgcc aaacagcgta
     3121 cgcgcctgac ccggccagga gtcggtgatc accaggctgc cttcggtagc gccttccagc
     3181 gggttacctt cgttatcgac cagcgccggt tgcacgccga agaacggacg tgttgccgaa
     3241 ccggctttca gctcggtagc gccaggcagc ggcgtgatca tgaaaccgcc agtttcggtc
     3301 tgccaccagg tatcgaccac cggacatttc tcgttgccga tttttttcca gtaccactcc
     3361 cacgcttccg ggttaattgg ctcgcccacg gaaccgagaa tgcgcagcga cgaacggtcg
     3421 gtaccctcga tcgctttatc gccttccgcc atcagcgcgc ggatcgccgt gggtgcggta
     3481 tagagaatat tgacctgatg cttgtccacc acctgcgcca tacgggcagg cgtcggccag
     3541 ttgggtacgc cttcaaacat cagcgtggtc gcaccgcagg ccagcgggcc gtacagcaag
     3601 taactgtgtc cggtcaccca gcccacatcg gcggtgcacc agtagatatc acccggatga
     3661 taatcaaaga catatttaaa ggtcagcgcc gcgtacacca gataaccgcc ggtagtatgc
     3721 agcacacctt ttggcttacc ggtagaaccg gaggtgtaga gaataaacag cggatcttcg
     3781 gcgttcatct cttccgcctg gtgctgatcg ctcgcttgct caaccaggtc gtgccaccac
     3841 aggtcgcgcc cttcctgcca gtcaattttc ccgccagtac gcttcagtac caccacatgc
     3901 tctacgctgg tgacgttcgg gtttttcagc gcgtcatcaa cgtttttctt cagcggaata
     3961 ctgcgcccgg cacgcacacc ttcgtcggaa gtgatcacca gtcgtgagtt ggaatcaata
     4021 atgcgcccgg caacggcttc cggcgagaag ccgccgaaaa tcaccgaatg caccgcgcca
     4081 atgcgggcgc aggccagcat cgcaaccgcg gcttccggca ccatcggcat ataaatcgcc
     4141 accacatcac cttttttaat gcccagctcg agcagggtat tggcgaagcg gcacacgtcg
     4201 cggtgcagct ctttatagct gatatgtttg ctctggctgg cgtcgtcgcc ttcccagatg
     4261 atggcggtgc gatcgccgtt ttcttgcaga tggcggtcaa ggcagtttgc cgccagattc
     4321 agcgtgccgt cctcgtacca tttaatggac acattaccgg gggcaaagga ggtatttttc
     4381 accttctggt acggtttgat ccagtcgaga atttttccct gttcgcccca gaaggtatca
     4441 ggtacgttaa tagattgttg atacatcgcc tcgtactgct gagggtttat caggcaacgg
     4501 tctgcgatgt tggcaggaat ggtgtgtttg tgaatttggc tcatgctttt gttctccttg
     4561 taggatgtta ataatatgtg gcataagcgt taaatgtagg ggtattggca gttttgttta
     4621 gtatttgggc gacagatcac gcaaaagtag aattgtgcaa ataaacggca gggtaatttt
     4681 tgaaggtcac caacaaaagt tgattaattc tttgaggaac atgcagttat gcatgctgtt
     4741 gaaaaagagg aagatactga ctaactctaa agtggtattt tacatgcact tacaattgat
     4801 taaagacaac attcacagtg tggttatatg ttacacatag gggcgagcaa tgtcatgaca
     4861 gtgtaggtgc ggttactgtc gtgaaaacaa taaaaaccgc cattgcaaca atggcgcaat
     4921 tcggatgaag cccctatgac aaggataaaa ataaacgcac gccgtatctt cagcttattg
     4981 attccttttt tctttttcac ttctgttcac gctgaacaaa cggcagctcc cgcaaaacct
     5041 gtaactgtgg aagcgaagaa tgaaaccttt gccccgcagc atcccgatca atatctctcc
     5101 tggaaagcca cctcggaaca gtcagagcgt gttgacgccc tggcggaaga tccacggctg
     5161 gtgatcctgt gggcggggta tcccttctcg cgcgattaca acaagccgcg tggacatgcc
     5221 tttgctgtga ccgatgtgcg tgaaaccctg cgtaccggtg cgccgaaaaa cgctgaagat
     5281 ggtccgctac cgatggcgtg ctggagttgt aaaagcccgg atgtggcgcg tctgatccag
     5341 aaagacggcg aagatggcta cttccacggt aagtgggcgc gcggcggccc ggaaatcgtc
     5401 aacaacttag gttgtgccga ctgccataac accgcctcac cagagttcgc caaaggcaaa
     5461 ccggagttaa ccctttcccg tccgtatgcg gctcgcgcga tggaagccat tggtaaacct
     5521 tttgagaaag ccggacgttt cgaccagcaa tcgatggttt gcggtcagtg ccatgtggag
     5581 tattacttcg acggcaaaaa caaagcggtt aaattcccgt gggatgacgg catgaaagtc
     5641 gaaaatatgg agcagtatta cgacaaaatt gccttctctg actggactaa ctccctgtcg
     5701 aaaacgccaa tgctgaaagc gcagcacccg gaatatgaaa cctggacagc gggcattcac
     5761 ggtaaaaaca acgtgacctg tatcgactgc catatgccaa aagtgcagaa cgccgaaggc
     5821 aaactctaca ccgaccataa aattggtaat ccgtttgata acttcgccca gacttgtgcg
     5881 aactgccata cccaggacaa agctgctttg caaaaagtgg tcgcggaacg taagcagtcg
     5941 attaacgacc tgaaaatcaa ggttgaagat caactggttc acgctcactt cgaagcgaaa
     6001 gcagcgctgg atgcaggcgc gacggaagcc gaaatgaagc cgattcagga cgatatccgt
     6061 catgcccagt ggcgttggga tctggcgatc gcttcccacg gtattcatat gcacgcaccg
     6121 gaagaaggtc tacggatgct cggtacggcg atggataaag cggcggatgc acgcaccaaa
     6181 ctggcgcgcc tgctggcgac caaaggcatc acccatgaaa tccagatccc ggatatctcg
     6241 accaaagaga aagcccagca ggccattggc ctgaacatgg aacaaatcaa ggccgagaag
     6301 caggacttca tcaaaacggt gatcccgcag tgggaagaac aggcacgtaa aaatggtctg
     6361 ttaagccaat aaccccgttc cgcctcgcaa ggggcggaaa acacaatgga gtgaatatga
     6421 gcgtattacg ttcgttatta actgccgggg tgctggcgtc gggcttgttg tggagcctga
     6481 acggaattac cgctacccct gcggcgcagg catctgatga tcgctacgaa gttacccagc
     6541 agcgtaaccc ggatgccgcc tgtctggact gtcataaacc agataccgaa ggtatgcatg
     6601 gaaaacatgc ttccgttatc aacccgaata acaaactgcc ggtcacctgc accaactgtc
     6661 acggccagcc atcaccgcaa caccgcgaag gggtgaaaga tgtgatgcgc tttaacgagc
     6721 cgatgtacaa ggttggagag cagaacagcg tctgtatgtc ctgtcatctg ccggaacagt
     6781 tgcaaaaagc gttctggccg cacgatgtcc acgtaaccaa agtggcgtgc gccagctgcc
     6841 attccctgca tccacagcaa gatacgatgc agacgttaag tgacaaagga cggattaaga
     6901 tttgcgtcga ttgccacagc gatcagcgca ccaatccgaa ctttaacccg gcgtctgttc
     6961 cgttgcttaa ggagcagcca tgacctggtc tcgtcgccag tttctcaccg gcgtcggcgt
     7021 gctggcagcc gtcagcggca ccgcagggcg cgtggtggcg aaaacgttga atatcaatgg
     7081 ggtgcgttac ggcatggtgc atgacgagtc gttatgcatc ggctgtaccg cctgtatgga
     7141 tgcttgtcgg gaagtgaaca aggtgccgga aggcgtctcg cgcctgacga ttattcgtag
     7201 cgagccgcag ggcgaatttc ctgatgtgaa atatcgcttc ttccgtaagt cttgccagca
     7261 ctgcgatcat gcgccgtgcg ttgacgtctg cccgaccggt gcgtcttttc gtgatgccgc
     7321 tagcgggatt gtcgatgtta atcctgatct ctgcgtcggt tgtcagtact gcatcgccgc
     7381 ctgtccgtac cgcgtgcgct ttatccatcc ggtcacgaag acggcggaca aatgcgattt
     7441 ctgccgtaaa accaatttgc aggccggtaa gctgcccgcg tgcgttgagg cttgcccgac
     7501 gaaggcgctg acgtttggca atctggacga tcctaacagt gagatttcgc aactgctgcg
     7561 ccagaagcct acttaccgct acaagctggc gctgggaacc aaaccgaagc tgtaccgcgt
     7621 accgtttaaa tacggggagg tgagccaatg acgcagactt ccgcatttca ttttgaatcg
     7681 ctggtgtggg actggccgat tgccatctac ctgtttttga ttggtatttc tgccggtctg
     7741 gtgacgctgg ccgtgctgtt acgtcgcttc tacccgcagg cgggcggtgc agacagtacg
     7801 ttgctgcgca ccacgctgat tgtcgggccg ggcgcggtga tcctcggtct gttgatcctc
     7861 gtcttccacc tgacaagacc gtggaccttc tggaagctga tgttccacta cagttttacc
     7921 tcggtgatgt cgatgggggt gatgctgttt cagctctaca tggtggtgct ggtgctgtgg
     7981 ctggcgaaaa tctttgaaca tgatttgctt gccctgcaac aacgctggtt gccgaagctg
     8041 gggatcgtgc aaaaggttct gagcctgctg acgcccgttc atcgcggact ggaaacattg
     8101 atgctggtgt tggcggtgtt gttgggggct tataccggct ttctgctgtc ggcgctgaaa
     8161 tcgtatccgt tcctcaataa cccgatcctg ccggtgctgt tcctcttctc cggcatctcg
     8221 tccggtgcgg cggtggcgct gatcgccatg gcgatacgcc aacgcagtaa cccgcattcc
     8281 acggaagcgc agtttgtaca ccgtatggaa atccccgtgg tatggggtga aatcttcctg
     8341 ctggtggcat tttttgtcgg tctggcgctg ggcgatgacg gtaaagtgcg tgcgctggtg
     8401 gcggcattag gtggcggttt ctggacgtgg tggttctggc ttggtgtcgc cgggctgggg
     8461 ctgattgtgc caatgttgct caaaccgtgg gtcaatcgca gttccggcat tcctgccgtg
     8521 ctggcggcgt gtggggccag tctggtcggc gtgttgatgc tgcgcttttt cattctctac
     8581 gccggacagt tgacggtggc gtaagccaga aaagaggtgg tttctggacg tattccttcc
     8641 tgaagtcggt tttctggcgt tgttgttaag tctcggggtc aacgtgttga ccccgttgac
     8701 ggcctttgcg ggagtgcggt tgcgctggcc tgccatgatg cgactcactt gcatcggcat
     8761 tctggcgcag ttcgcgctcc tgctgctcgc ctttggcgta ctgacgtatt gttttctcat
     8821 cagcgatttc tcggtcattt atgtcgcgca acatagctat agcctgctgt cgtgggaact
     8881 caagctggca gcggtgtggg gcggccatga aggttcgctg ctgctttggg tgctgctgct
     8941 ttccgcctgg agcgcgctgt ttgcctggca ttatcggcag caaaccgatc cgctatttcc
     9001 gctgacgtta gccgttttat ctctcatgct cgccgcactg ctactgtttg tggtgctgtg
     9061 gtccgatccc ttcgtgcgga tatttccacc agcaatcgaa ggccgcgatc tcaatccgat
     9121 gctgcaacat cccggtctta tctttcatcc accgctgctt taccttggct atggcggttt
     9181 gatggtagcg gcgagcgtgg cgctggcgag tttactgcgc ggcgagtttg atggtgcctg
     9241 cgccagaatt tgttggcgct gggcactacc tggctggagc gcattaacgg cggggatcat
     9301 cctcggttcc tggtgggcct attgcgaact gggctggggc ggctggtggt tctgggatcc
     9361 ggtggaaaac gcctctttat taccctggct ttctgccact gcgctgctgc acagtttgtc
     9421 cctgacacgc cagcgggaga ttttccgcca ctggtcgctg ttgctggcga tagtaactct
     9481 gatgctgtcg ctactgggta ccttaattgt ccgttctggc attctggttt cggttcatgc
     9541 gttcgcactg gataacgtcc gcgccgtgcc gttgttcagc ctgtttgcac tgattagcct
     9601 tgcgtctctg gctctgtatg gctggcgagc gcgggacggt ggtgcggtgg tgcgtttttc
     9661 ggggttatcg cgggaaatgt taatcctcgc tacgctgttg ctgttttgcg cagtgctact
     9721 gatcgtgctg gtgggaacgc tttatccgat gatttacggc ctgctgggct ggggacgcct
     9781 ctccgttggc gcgccgtatt ttaaccgcgc gacgttaccg tttggcctgt tgatgctggt
     9841 ggtgattgtg ctggcgacgt ttgtctctgg caaacgcgtg cagcttccgg cgctggtagc
     9901 tcatgcgggc gtgctgttat ttgccgcggg gatcgtggtt tccagcgtca gccgtcagga
     9961 gatcagcctg aatttacagc cgggtcagca ggtgacgctg gcaggataca ccttccgttt
    10021 tgagcgcctc gatctgcaag ccaaaggcaa ttacaccagc gaaaaagcga tagtggcact
    10081 gtttgaccat cagcaacgca ttggtgaact gatgccggag cggcgttttt acgaagcacg
    10141 tcgtcagcaa atgatggaac cgtcaattcg ctggaacggc atccatgact ggtatgcggt
    10201 catgggtgaa aaaaccggag cggatcgtta cgcttttcgc ttgtatgtac aaagcggtgt
    10261 gcgctggatc tgggggggag gattgttgat gattgcgggc gcattgttaa gcggatggcg
    10321 ggggaggaag cgcgatgaat aaaggacttc tcacgctact gctgttattt acctgttttg
    10381 cgcgcgctca ggtcgtagac acctggcaat tcgccaatcc gcaacaacag caacaggcgt
    10441 taaatattgc cagccagtta cgttgtccgc agtgccagaa tcaaaactta ctggaatcca
    10501 acgcgccggt ggcagtcagt atgcgccatc aggtttacag catggtggcg gaggggaaga
    10561 acgaagtcga aatcattggc tggatgaccg aacgctacgg agattttgtt cgctataacc
    10621 caccgttaac gggtcagacg ctggtgttgt gggcgctgcc agtggtgttg ttactgctga
    10681 tggcactgat cctctggcga gtgagggcaa agcgatgaaa gagcccaaaa taccggtgaa
    10741 aatgctgaca acactcacga ttttgatggt atttctctgt gtcggcagtt atctgttaag
    10801 tccaaaatgg caggcggtac atgcggaata tcagcgtcag cgcgatccgc tacatcagtt
    10861 tgccagccag caaaccccgg aagcgcagct tcaggcattg caggataaaa tccgcgctaa
    10921 tccacaaaac agcgaacaat gggcgttact gggcgagtac tatctgtggc aaaacgatta
    10981 cagcaattcg ctgctggcgt accgtcaggc gttgcaacta cgtggtgaga acgctgaact
    11041 gtatgcggcg ctggcgacgg tgctttatta ccaggccagc cagcatatga ccgcccagac
    11101 tcgcgcaatg atcgacaaag ccctcgcgct ggactctaac gaaatcaccg ctctgatgct
    11161 gctggcttcc gatgcgttta tgcaggcgaa ttacgcgcaa gccatcgaac tatggcaaaa
    11221 agtgatggat ctcaactcac cccggatcaa ccggacacag ctggtcgagt cgattaatat
    11281 ggcgaaattg ttgcagcgga gatcagatta atcatctggt gatgaagatt atgttattga
    11341 gttgtataaa aaataatcga atgaacatat gccaaaaata atcaccaatc agtattattg
    11401 cagattaaac aaataaaaat ctttccataa caaatggtta ttcattaatc ctgccataaa
    11461 ctcgcgttat tatgcattaa tgcagcgaaa agctctgttg ttaaagggtt gcgcaacata
    11521 ccgcgcaaat gatactgatc ataagcgtta aaaaaatcta caaaccaacg caacacaatt
    11581 catgccctgg cagtatgtca cgttctcgcg tttctgaacg gggaacggcg ctccattgag
    11641 gaagttattc tgatgaaaag tataaaattc agcctggcct ggcagattct gtttgctatg
    11701 gtgctgggca ttctcctggg aagctacctg cactaccata gcgacagccg cgactggctg
    11761 gtcgtcaatt tgctctctcc ggcgggtgat atcttcatcc atctgatcaa aatgattgtt
    11821 gtgccgattg tgatctccac gctggtggtg ggtatcgcgg gtgttggtga tgccaaacag
    11881 ctcgggcgta ttggcgcgaa aaccattatt tacttcgagg tgatcaccac cgtcgccatc
    11941 attttgggga tcactctggc gaacgtcttc cagcccggtg ccggggtgga tatgtcgcag
    12001 ctggcgaccg tcgatatctc gaaatatcag agcactacgg aagcggtaca aagcagttcc
    12061 cacggcatta tgggcacgat tttgtcgctg gtgccgacga acattgtggc gtcgatggcg
    12121 aaaggcgaaa tgctgccgat catcttcttc tcggtgctgt ttggtctggg gctttcctcc
    12181 ctgcccgcga cgcatcgtga accgctggtg accgtgttcc gctccatctc tgaaaccatg
    12241 tttaaagtga ctcacatggt gatgcgttat gcaccggtgg gtgtgtttgc gctgattgcg
    12301 gtgacggtgg ctaactttgg tttctcttct ctgtggccac tggcgaaact ggtgctgctg
    12361 gtgcatttcg ccattctgtt cttcgcgctg gtagtgctgg gaattgtggc gcgtctgtgc
    12421 gggttaagcg tctggatcct gattcgtatt ctgaaagatg agctgattct ggcgtactcc
    12481 actgccagct ctgaaagcgt actgccgcga attattgaga agatggaagc ctacggagca
    12541 ccggcgtcga tcaccagctt cgtggtgccg accggttact cttttaacct tgatggttcg
    12601 acgctgtatc aaagtattgc cgctattttc atcgctcagc tgtatggcat tgacctgtcc
    12661 atctggcagg aaatcattct ggtgctgacg ctgatggtga cctcgaaagg gattgctggc
    12721 gtgccgggcg tgtcgtttgt ggtgctgctg gcaacgctgg gtagcgtagg tattccgctg
    12781 gaaggcctgg cgtttattgc tggtgttgac cgtatcctcg acatggcgcg tactgcgctg
    12841 aacgtggtgg gtaatgcgct ggcggtgctg gtgattgcca agtgggaaca caaatttgac
    12901 cgcaaaaagg cgctggctta tgagcgtgaa gtgctgggca aatttgataa aactgcggat
    12961 caataattga agattgccgg ggttatccac ccggcaatgt gtggatgcct gatgcgacgc
    13021 tggtgcgtct tatcaggcct accttaaccg ttggttaatt tttcaaactc ttcacacccg
    13081 gtatcaaacc cttccataca gctcaggttc aaccagtgca gcgccttttg tttgttcttc
    13141 tcgataaaac cttcctcacc gtttaagaac atcattcccg cccagtactc ggaataaccg
    13201 gttcgggaaa ttgcagagct gcgtttgaaa taccaggttg ccttgtcatc gtcggcctta
    13261 atgcccacgc cgttggcgta aatcaaacca agcagcattt gggcatcgac ggcggagtca
    13321 ttctccagat cttccgaggc gttttctaac agcgaaatcg cctttggata atccggttta
    13381 cccgcttgag tatttaccag aatatgcgcc agcgttactt caccttcttt actgcccgct
    13441 tgcgccgctt tctctgcaag aacttttgct tgtggatagt ccagactgac cggattggtg
    13501 attttgatct gcgccagcag cgcgcaggca tcagcatcac cgctgtcggc ggctttctgt
    13561 gcccaatatt cggctttgct caaatcgccg gagctaaacc agctgtcggc aagaaaatat
    13621 tgtgcgcgtc ggtccccggc ctctgctgcc tttaaatact ggctgccagg ctcggagtcg
    13681 ttcgcgtggg caaagaatgt caaaaacaac atcaatgcga taattttttt cattttgata
    13741 atacgattgg gtagggagta accagtataa agagttcgaa tggataaaaa aacagcctcc
    13801 gaaaggaggc tgtagaaagg actgtattac gccagtgccg cttcgcgcag gcgagttttc
    13861 aacttgttgt actcgtcaat cacgtactgc tcggcggcgc gctgatcggc gatcggctcg
    13921 acgcgcacgg cgcagtattt gtattccggc gttttcgtaa tcgggcttaa gttttcggta
    13981 accagctcgt tacaggcacc aatccaccac tggtaggtca tgtaaatcgc ccctttgttc
    14041 ggacgatcgc tgacctgcgc acgggtgata attttgcctt tacgcgagtg cacccaaacc
    14101 aatgcctcat cttcaatacc cagacgtttg gcgtcttcgg tattgatttg tgcgtagcca
    14161 ggttcatcag ccagcgccgc cagtgccgca cagttaccgg tcatcgaacg gcaagagtag
    14221 tgaccaactt cacgcaccgt tgacagtacc atcgggtact cgtcggtgag tttgtcgatt
    14281 ggcgctaccc agtcgcaggt gaagaactgt gccagaccgt tcggggtatc aaacttctct
    14341 ttaaacagat aagaagtccc ctgatcggca tctgaagtat cgcggcaagg ccactgaatg
    14401 aagcccagtt cgcccatttt ctcgtaagtc gcaccgtaga aatccgggca cagatgacgc
    14461 aactcatccc agatctcctg ggtgttgttg tagtgcatcg gataacccat acgggtggcg
    14521 atttcactga tgatttgcca gtccgttttc agatcccatt tcggttcaac tgctttgaag
    14581 aaacgctgga agccacggtc agccgcagta aacacgcctt catgctcgcc ccacgacgtt
    14641 gacggcaaaa tgacatccgc cgccgacgcg gttttggtca taaagatgtc ctgaacgata
    14701 accagttcca gatcttcaaa ggctttacgt actgccgaca gctccgcgtc agtttgcagc
    14761 ggatcttcgc ccataatgta cgcggcacgc acttcgccat gcgctgcgcg gtgcggcagc
    14821 tcgctgatgc gataaccggt atgagcaggc aggctttcca cgccccaggc tttggcgaat
    14881 ttctcgcggt tagccggatc tttcacgtac tggtatcccg gatacgtatc cggcagcgcg
    14941 cccatatcgc aggcaccctg cacgttgttc tgaccacgaa ccgggtttac acccgcatgc
    15001 ggcttaccga ggttaccggt cagcatcgcg aggctggtca gagaacgcac ggtttccacg
    15061 ccctggtaga actgggttac acccatgccc cacaggatgg cggcgctttt cgcctgggca
    15121 tacatccgtg ccgcctgacg aatctcactg gcgctgacgc cggtgatatc ttcaaccgac
    15181 tccggcgtgt agccttcaac gattttacga tactcttcaa agccttctgt acgggaagcg
    15241 acgaacgctt tgtcgtacag attttcttca ataatgacat ggcccatcgc attcaacagc
    15301 gcgatgttcg agccgttttt cagtgcaatg tgcatgtcag caatgcgcgc ggtttcaatt
    15361 ttgcgcggat cgcagacgat aattttcgcc ccgttacgtt tagcgttaat tacgtgattc
    15421 gccacgattg ggtgggaatc cgccgggttg tacccgaaaa cgaacactaa atcggtatta
    15481 tcaatttcgt taatagcatt gctcattgcg ccattaccga ccgattggtg cagacctgca
    15541 accgatgggc cgtgtcagac acgagcgcag cagtcaacgt tattggtacc aataacggcg
    15601 cgcgcaaatt tttgcattac atagttggtt tcgttacccg taccacgcga ggagccggtc
    15661 gtctggatgg catccggacc gtacttctct ttgatggcgc tcaggcgctc ggcaacgtaa
    15721 ttcagtgcct catcccagga aacaggttcg agtttgccgc cacgctggcg acggatcatg
    15781 ggggttttca ggcgcggggt caggatctgg gtatcgttaa tgaaatccca gccataataa
    15841 cccttcagac acagggtacc ctggttggtt ttcccctgcg ctgcctccgc ccggacgatt
    15901 ttgccgttat cgacgaccag gttgattttg caacctgatg cgcaataggg gcaaaccgtg
    15961 acgacttttt tcatcggtct cgctccagtt aatcaaatca cgcatacgcg ctctcgacta
    16021 cagtatgcat cttttatgcc acattttatt tggggtcatt ccctgatatt acgggcacta
    16081 tttattcaaa actctgacga aaaacaggct gtcgtcagtt ttgacgtgac gaaacgaaat
    16141 accgcgtgac agccatcacg cggcagacat tttatttttt ctcgacgacg ggagccgcct
    16201 gatacccgcc gcccagcgat ttcatcaact gaatgctttg gatcacccgg cggctgtcca
    16261 gcatcagtaa tgacatctct tcggcaagta ctggcaaccg ggcttcagtg gcctgtaagc
    16321 ggctggttaa gccgcgctga taggcggcct cggcagcgcg ctgggtgaag cgcgttgctt
    16381 ccacgcgttc agcctgcatt tctcgctcgt cgttgagcgt ttgcagacgc gtgccgttga
    16441 cggcaacgtc acgcaccgcg ttcagtactg actggttgta acgttcaatc atcatgttgc
    16501 tggcggcgcg cgtgccttcg agattggcat tcaaccgtcc accgtcaaac agcggcaatt
    16561 tcagacccgg gatgaagttg aactggcgac tggttttttt gaataaggta tccaggtgga
    16621 tggcatcaag gccgaaaaac gctttgatat caaagctcgg gtagaacagc gcccgcgcgg
    16681 aatccacctg atctaatgac gcctgaacat accagcgcat ggcttgcaga tccgggcgtc
    16741 tggcgagcaa ctcataagag agtgtcgccg gaatgccggt ctggactcgt ggtaatgcca
    16801 ccggtttgat ctccggcata tcgctggccc ccgcgccaat caatgcacgc agagattctc
    16861 gcgtttcggt gatttgccct ttgacggcag caatttgttt atcgaccgcc agaatctgtg
    16921 cccgcgcgcc ctggaaaggc acttgtgctt ccagaccgtg cgccacttta ctctggtgcg
    16981 ctttcaccgc gtaatcaatc acatcgcgag tttgttctaa cagatcgagc atctgatagc
    17041 tggcctgcat actgtaataa agctgcgcta cgcccgtggt cagcgatagc tctactgctg
    17101 cggtttctgc cagcgtggca ttatgcgcgc caatggcggc ggcaaccgct gagcgatgca
    17161 caccccacaa atcaagatcc agtccggcaa acaaagctac tgtggcttcc gtatagtacg
    17221 gcccgtccat acccagtgcg ggcgcatcca tcgcataagg gcttaaaaag ccgttcgccg
    17281 agacgcgttg gcggttgagc atccctaacg ctgcgacctg taattgtgaa ccggcatcta
    17341 acaaatcggc ctgggactgc gctttttctt cccgcagttt cgcttcggcg agggtgtgtg
    17401 aaccacttag cgtccgttgg agcagcgcat ccagctgcgg gtcattgagt tgtttccacc
    17461 actgcgcctg cggccagccg gagctggcaa gatgaatatc gtcggccagt ttgatttgtt
    17521 ccggtttgag ctgttgatga ggtgcagaat ccttacgtac cagggcacag ccggaaatca
    17581 gcgtcgtgct gccgagaatg ctgcacaaca gcagacgtga aagttgacga ttgatcattg
    17641 cgtggctcct tgtgcctgtt ccgtggcggg cgttaatgcc ggggctgtcc agtctggcaa
    17701 gcgggcgaaa agctggcaga cgtgttgctc ctgttttaat aaggtgggca ggatggcctg
    17761 cgaggcgggt gtctcttcaa gcgttatttg aggagaacgg ctgagtgcgg ttgccagacc
    17821 ggcagcgtat ttctccaggg cgtcggcaaa ctgagctgct ctgtccggct gtagtgcgtt
    17881 atccagcgcc tgcgccgagt tccaggtggc atcccaggcg tgaagaatat cgcggccctg
    17941 acgaataacc gtttgcgaac gttcaatcag taaggcgcgt tcttcgctgt ccagttgacg
    18001 ctccagcgcc acgcgttggc acatctcttc acaggcatta aacgccgcat gcagaccgat
    18061 acgaatttgc agataagtgc gcagagccgt gacttcctgc tggcgtggga tccgcattac
    18121 tttacttaac atacccagcg tgccagccag tttttgcggc agtgtgcgcg cttcactttc
    18181 aggccagaca aaggtgtaaa tcaccgcgga caccacggta ccaatgatga tacccagggc
    18241 gcgatcgcga atttccacca ggtcgtacac tgggccaaaa acgttttcga gcgtggcgag
    18301 cgcgaaggtg accaccatct gtgtgccgat ataagaagag cgttcagagc tggtggcaat
    18361 ccatgcgccc aacaggaaaa tcggtgccag cacaaacagc aattcgacaa tattgtccag
    18421 ccagggcatg accagtagcg tgaataacag cgccagaatc gcgccgcaaa aggccccgcc
    18481 aaaacgcagc accatcttct ggtacgacga accgacattt gggttagcga cgatcacgca
    18541 tgtcagcata caggtgtgaa tgccttccca gtccacgccg ctgtaaaagg tgtaacagat
    18601 caaacaggcg agcagcgttt ttaccgcgta gcgcatatag tctggattgg taaaagcatc
    18661 ggcggccatt gatggcggtt tggctgcggg cgttggcggc gtattcgggt ccatctgacc
    18721 cagttgtaac aacgtctggc agatattctc caggttacat tcccgtgccg ccatcgcttc
    18781 actttcactg atccgccagt cgctttgcca gcactgacct tcagcaacgg catgctgcag
    18841 cttattgatt tctgaggcta atttttgccg aaattcaata attgcctgag aatcagcaaa
    18901 agaggtggga tcgtagcgat tcagcgtcga gtaaatgtag gttaccgttg ccacgcagct
    18961 ttgccaccat gcgctttgag ttcgccagtt ggcatcgtcc gcgagacaaa agacattcag
    19021 tttttgcagc gccagcgcct ctctttcaat ccgcgtttcg ggtagcggtg cgaggctgtc
    19081 cgtcaggtgg ctaatggcat catcaagccg atcattaagc gcctgatgca tttgcgaaat
    19141 ggcacgactg ggaaaccaca gcacgccgat taacgtcatc agcaaggttg gataaaggcc
    19201 aacaacgata caccacagcg ttaagcgcac gaccacttcc ggatagtcga gcatggcggg
    19261 gaaggtttgc ccgtaaatgg cgacaatggc gactgcgaaa aagaccagtc ccagccgatg
    19321 ggtgcgcatc aaaaacatac agcccatcag gatcggcccg gcgatgaaca atcggatcaa
    19381 cggttcgccg tatgaccatt tgtagatcaa aaacaggctg ccgatctcca gcatcgtggc
    19441 gaccacaaat aagatcgcga caaatttggt gtaaaacgcg ttcgactgaa taccgtaaaa
    19501 cagcactgcc agcgataacg ccacgaaagg gatctcaaag gtcatcgaga tcagaatcac
    19561 cagcaggcag cctacccaga gttgcacggt ctgcggcacg cggcctggtc gccgctcgct
    19621 taactcttca tgaaagaacg ccagcagcct gaccaccggt aatggcaggg agttgagcgc
    19681 gctcattatt gcggctcaag attagcgact gccgaagcgc cgatgcggaa catttccggg
    19741 tcaggtttat cgaccatgat tttgaccgga aaacgctggg caacgcggac ccagttaata
    19801 gagcgagaaa ctttcggcag gccgcccagc accaggccgc cgtcatccgg tagcacgccg
    19861 tagccaatcg aatccacttt accctcgaag gttttgccgc tgtcactcat caggcgaatc
    19921 gttgcgggtg tacctgagcg aatatttttc agatcggttt cgcggaagtt ggcgatcacg
    19981 taccagtgac gagtgtcgat tagggtaaaa atagggcgca tggcagaagc aaattgcccg
    20041 acggaggttt tgagggaaat gacccggcca tcaaacggcg cgcgaacggt cgccatttcc
    20101 agatgcagtt tggtcagggc aatatccgct tcgaccgccg cacgctgggc aactaatgca
    20161 tccacgccgc tgacggcgct ggcggctgac tgcgcctgca acaatacagc attaagatcg
    20221 gcttctgcag cgcgctgcgc ggtcctcgca cggtcaacgt cttccgctga gacaaaacct
    20281 tctttcagta atggctcggt gcggcgtaat gtatctgtgg cctgtttcgc ggcggcacgg
    20341 gctttttcta ccgtggcgtt aaccgagtcg gcaccaaact gttgcgcgtc aacgctacgc
    20401 tgggtgagca taatttgctt atccagcgcc gcgagggagg cttcagcttt cgccagattg
    20461 gcttcgtacg ggcgcgggtc aatgcggaac agcaaatcgc cctgtttgac tgcctggttg
    20521 tcggtgaccg ccagttctac aatgcgaccg ctgacttccg gcaccacatc aatggtatct
    20581 gctgacgcgt aagcgtcatt agttgatggc gcactgtcta cgcgccagat aacgaaaaca
    20641 agggcaacca gcgccaacgc aaccactaac agagcaggga atttactgcg aggagctttt
    20701 ttcggcgtac tttccatgag cgcgtctcaa atagattaga aaaatgccag ccagaacagc
    20761 atggcgtaga gagcaaaaag ggcggtataa ataattccga caaatgccag attgatattt
    20821 gcccgctgaa taactcgcct cgtgatgagc atcaaaataa ggctggcaat ggcgcagaaa
    20881 aaccagctgg gataataagc accgatcacc gggattgccg gagataatga gcatcccgaa
    20941 accatgaaga cgggaattat ccaggctgtt tttttgagtt gcatcgccat gcgagagaga
    21001 actgttggca tattaaatac atcctgtctg ctgcgaacaa tatctgtagt gaatatcaat
    21061 atccattgaa ggcgtcagta tggctatttt ctcgatgaat aaaaatagct tta
//
LOCUS       DAZFWL020000080        20569 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFWL020000080 DAZFWL020000000
VERSION     DAZFWL020000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.080"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(381..695)
                     /gene="fliE"
                     /locus_tag="ACHKNP_RS15420"
                     /old_locus_tag="pgaptmp_003084"
     CDS             complement(381..695)
                     /gene="fliE"
                     /locus_tag="ACHKNP_RS15420"
                     /old_locus_tag="pgaptmp_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGU4705210.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHATLDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            910..2568
                     /gene="fliF"
                     /locus_tag="ACHKNP_RS15425"
                     /old_locus_tag="pgaptmp_003085"
     CDS             910..2568
                     /gene="fliF"
                     /locus_tag="ACHKNP_RS15425"
                     /old_locus_tag="pgaptmp_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HGU4705211.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2561..3556
                     /gene="fliG"
                     /locus_tag="ACHKNP_RS15430"
                     /old_locus_tag="pgaptmp_003086"
     CDS             2561..3556
                     /gene="fliG"
                     /locus_tag="ACHKNP_RS15430"
                     /old_locus_tag="pgaptmp_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGU4705212.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3549..4235
                     /gene="fliH"
                     /locus_tag="ACHKNP_RS15435"
                     /old_locus_tag="pgaptmp_003087"
     CDS             3549..4235
                     /gene="fliH"
                     /locus_tag="ACHKNP_RS15435"
                     /old_locus_tag="pgaptmp_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HGU4705213.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQMPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4235..5608
                     /gene="fliI"
                     /locus_tag="ACHKNP_RS15440"
                     /old_locus_tag="pgaptmp_003088"
     CDS             4235..5608
                     /gene="fliI"
                     /locus_tag="ACHKNP_RS15440"
                     /old_locus_tag="pgaptmp_003088"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGU4705214.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5627..6070
                     /gene="fliJ"
                     /locus_tag="ACHKNP_RS15445"
                     /old_locus_tag="pgaptmp_003089"
     CDS             5627..6070
                     /gene="fliJ"
                     /locus_tag="ACHKNP_RS15445"
                     /old_locus_tag="pgaptmp_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HGU4705215.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6067..7194
                     /gene="fliK"
                     /locus_tag="ACHKNP_RS15450"
                     /old_locus_tag="pgaptmp_003090"
     CDS             6067..7194
                     /gene="fliK"
                     /locus_tag="ACHKNP_RS15450"
                     /old_locus_tag="pgaptmp_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGU4705216.1"
                     /translation="MIRLAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAVPVLSAPLGSHEWQQSLSQHISLFTRQRQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLLVPVSIQGRVTGNSGVDIFA"
     gene            7299..7763
                     /gene="fliL"
                     /locus_tag="ACHKNP_RS15455"
                     /old_locus_tag="pgaptmp_003091"
     CDS             7299..7763
                     /gene="fliL"
                     /locus_tag="ACHKNP_RS15455"
                     /old_locus_tag="pgaptmp_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HGU4705217.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7768..8772
                     /gene="fliM"
                     /locus_tag="ACHKNP_RS15460"
                     /old_locus_tag="pgaptmp_003092"
     CDS             7768..8772
                     /gene="fliM"
                     /locus_tag="ACHKNP_RS15460"
                     /old_locus_tag="pgaptmp_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HGU4705218.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8769..9182
                     /gene="fliN"
                     /locus_tag="ACHKNP_RS15465"
                     /old_locus_tag="pgaptmp_003093"
     CDS             8769..9182
                     /gene="fliN"
                     /locus_tag="ACHKNP_RS15465"
                     /old_locus_tag="pgaptmp_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HGU4705219.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9185..9550
                     /gene="fliO"
                     /locus_tag="ACHKNP_RS15470"
                     /old_locus_tag="pgaptmp_003094"
     CDS             9185..9550
                     /gene="fliO"
                     /locus_tag="ACHKNP_RS15470"
                     /old_locus_tag="pgaptmp_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HGU4705220.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            9550..10287
                     /gene="fliP"
                     /locus_tag="ACHKNP_RS15475"
                     /old_locus_tag="pgaptmp_003095"
     CDS             9550..10287
                     /gene="fliP"
                     /locus_tag="ACHKNP_RS15475"
                     /old_locus_tag="pgaptmp_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGU4705221.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10297..10566
                     /gene="fliQ"
                     /locus_tag="ACHKNP_RS15480"
                     /old_locus_tag="pgaptmp_003096"
     CDS             10297..10566
                     /gene="fliQ"
                     /locus_tag="ACHKNP_RS15480"
                     /old_locus_tag="pgaptmp_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HGU4705222.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10575..11360
                     /gene="fliR"
                     /locus_tag="ACHKNP_RS15485"
                     /old_locus_tag="pgaptmp_003097"
     CDS             10575..11360
                     /gene="fliR"
                     /locus_tag="ACHKNP_RS15485"
                     /old_locus_tag="pgaptmp_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGU4705223.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLADTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLILTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            11650..12273
                     /gene="rcsA"
                     /locus_tag="ACHKNP_RS15490"
                     /old_locus_tag="pgaptmp_003098"
     CDS             11650..12273
                     /gene="rcsA"
                     /locus_tag="ACHKNP_RS15490"
                     /old_locus_tag="pgaptmp_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HGU4705224.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12317..12505)
                     /gene="dsrB"
                     /locus_tag="ACHKNP_RS15495"
                     /old_locus_tag="pgaptmp_003099"
     CDS             complement(12317..12505)
                     /gene="dsrB"
                     /locus_tag="ACHKNP_RS15495"
                     /old_locus_tag="pgaptmp_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HGU4705225.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            12668..12895
                     /gene="yodD"
                     /locus_tag="ACHKNP_RS15500"
                     /old_locus_tag="pgaptmp_003100"
     CDS             12668..12895
                     /gene="yodD"
                     /locus_tag="ACHKNP_RS15500"
                     /old_locus_tag="pgaptmp_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HGU4705226.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13193..14008
                     /gene="yedP"
                     /locus_tag="ACHKNP_RS15505"
                     /old_locus_tag="pgaptmp_003101"
     CDS             13193..14008
                     /gene="yedP"
                     /locus_tag="ACHKNP_RS15505"
                     /old_locus_tag="pgaptmp_003101"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HGU4705227.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQSSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAH"
     gene            complement(14005..15699)
                     /gene="dgcQ"
                     /locus_tag="ACHKNP_RS15510"
                     /old_locus_tag="pgaptmp_003102"
     CDS             complement(14005..15699)
                     /gene="dgcQ"
                     /locus_tag="ACHKNP_RS15510"
                     /old_locus_tag="pgaptmp_003102"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HGU4705228.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLHWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(15937..16119)
                     /gene="yodC"
                     /locus_tag="ACHKNP_RS15515"
                     /old_locus_tag="pgaptmp_003103"
     CDS             complement(15937..16119)
                     /gene="yodC"
                     /locus_tag="ACHKNP_RS15515"
                     /old_locus_tag="pgaptmp_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HGU4705229.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(16198..17115)
                     /gene="yedI"
                     /locus_tag="ACHKNP_RS15520"
                     /old_locus_tag="pgaptmp_003104"
     CDS             complement(16198..17115)
                     /gene="yedI"
                     /locus_tag="ACHKNP_RS15520"
                     /old_locus_tag="pgaptmp_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HGU4705230.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVAGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            17288..18208
                     /gene="yedA"
                     /locus_tag="ACHKNP_RS15525"
                     /old_locus_tag="pgaptmp_003105"
     CDS             17288..18208
                     /gene="yedA"
                     /locus_tag="ACHKNP_RS15525"
                     /old_locus_tag="pgaptmp_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HGU4705231.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIPDASSE"
     gene            complement(18197..18667)
                     /gene="vsr"
                     /locus_tag="ACHKNP_RS15530"
                     /old_locus_tag="pgaptmp_003106"
     CDS             complement(18197..18667)
                     /gene="vsr"
                     /locus_tag="ACHKNP_RS15530"
                     /old_locus_tag="pgaptmp_003106"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HGU4705232.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVLDEFRCVIFAHGCFWHHHHCYLFKVPATRTDFWLEKIGKNVERDRRDIS
                     HLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(18648..20066)
                     /gene="dcm"
                     /locus_tag="ACHKNP_RS15535"
                     /old_locus_tag="pgaptmp_003107"
     CDS             complement(18648..20066)
                     /gene="dcm"
                     /locus_tag="ACHKNP_RS15535"
                     /old_locus_tag="pgaptmp_003107"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HGU4705233.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(20133..>20569)
                     /locus_tag="ACHKNP_RS15540"
                     /old_locus_tag="pgaptmp_003108"
     CDS             complement(20133..>20569)
                     /locus_tag="ACHKNP_RS15540"
                     /old_locus_tag="pgaptmp_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HGU4705234.1"
                     /translation="EFVQFPAEKIEAVCHAIAAHSFSAQIAPLTTEAKIVQDADRLEA
                     LGAIGLARVFAVSGALGLALFDGEDPFAQHRPLDDKRYALDHFQTKLLKLPQTMQTAR
                     GKQLAQHNAQFLVEFMAKLGAELAGENEGIDHKVIDAFSPAG"
BASE COUNT         4949 a         5455 c         5328 g         4837 t
ORIGIN      
        1 attgatcaat aatactgcgt cataagaaat ctctattaga caatagtaga cgctccttcc
       61 acatgcgcca caagaaacct ctattccagt gacacaatta cgcctgatta tttccctagc
      121 ccggaatatg aataaaaaca tcaacaaaga ttaaattatt caaacgttac tgttgcgtct
      181 gcggtcgtag caacatccga cgtggtgatg agtgttaaaa gagacactct gatgttcaga
      241 gggattatcg cgtttacgga tcgtggatgg cagaaaactt tcaggatcag gtatccattt
      301 taaaccagaa attgagtgag tttgccccat ccatgcccca agcggtgaga ttggaagtaa
      361 ttaacaacag gttacaaaac ctacacctgc atggacatca cttcctgata cgccgccacc
      421 agcttattac gcacctgaat ccccatttgc atagaaactg aggctttttg catatcggtc
      481 atcacatcgt ttaacgccac gccgggttca ccgagggtga atttttctgc ctgcgtgcgg
      541 gcagctgttt gtgtatcgct aatgcgatcg agcgtggcgt gcagctgccc ggcaaaacta
      601 atggtcggtt gcggcagtga atcctgcgca cgcgcactca tcgccgtagc ctgtaactgg
      661 ctgataaccc cttcaatccc ctgtatcgct gacattttcg tctcctggat aatttctggt
      721 agcaaagcct accagtaagt caataagata aaggcgctaa ataacaacaa aaaaacgggt
      781 ttattggcgg atagaaaaaa acgaaagcac aaataatggc agcgtcaatt tttcgagttt
      841 gctgacccgg gagtgagtct tgttccactt tgccaataac gccgtccata atcagccacg
      901 aggtgcgcga tgaatgcgac tgcagcccag acaaaatctc ttgagtggct taatcgcctg
      961 cgtgcgaatc cgaaaattcc attgattgtt gccggttccg cggcagtggc ggtcatggtc
     1021 gcactgatcc tgtgggcgaa agcccccgac taccgcacat tattcagcaa tctttccgat
     1081 caggatggtg gcgcaattgt cagccaactg acgcaaatga atattcctta ccgcttcagc
     1141 gaagccagcg gcgctattga agttccggca gataaagttc acgaactgcg tctgcgcctg
     1201 gcacaacaag gtttgcccaa aggcggcgcg gtcggtttcg aactactcga tcaggaaaag
     1261 tttggtatca gccagttcag cgaacaggtg aattatcagc gggcgctgga aggcgagctt
     1321 tctcgtacca tcgaaactat cggcccggta aaaggggcgc gcgtacatct ggcaatgccg
     1381 aaaccgtctt tattcgtccg tgaacaaaaa tccccttctg catcggtgac ggtaaatctg
     1441 ttacccggcc gcgcactcga tgaagggcaa attagcgcca ttgtgcatct ggtttccagc
     1501 gccgttgctg gtctgccgcc gggaaacgtc acgctggtgg atcagggcgg acatctgtta
     1561 acccagtcca ataccagcgg gcgcgatctt aatgatgctc agttgaaata tgccagcgat
     1621 gtcgaaggcc gtattcagcg gcgtattgaa gcaatcctgt cgcctattgt tggtaacggt
     1681 aatattcacg cccaggtcac ggcgcagctg gacttcgcca gtaaagaaca aacggaagaa
     1741 cagtatcgcc ctaacggtga tgaatctcat gcggcgcttc gttcacgcca gcttaatgag
     1801 agcgagcaaa gcggttccgg ttatccgggc ggcgtaccgg gggcgttgtc gaatcaaccg
     1861 gcacctgcga ataacgcgcc aatcagcacg cctccggcaa atcaaaataa ccgccagcag
     1921 caggcgagca ccaccagcaa tagcgggccg cgtagcacac agcggaatga aaccagtaac
     1981 tacgaagtcg atcgcaccat tcgtcatacc aaaatgaacg tgggcgatgt gcaacgtctg
     2041 tcagtcgcgg tagtggtgaa ttacaaaacc ttgccagacg gtaaaccatt gcctctcagc
     2101 aacgaacaga tgaagcaaat tgaagatctg acccgcgagg cgatgggctt ttctgaaaaa
     2161 cgcggtgact cgctcaatgt cgttaactcg ccgttcaata gcagtgacga aagcggcgga
     2221 gaactgccgt tctggcaaca gcaagcgttt atcgatcagt tgctggctgc cggtcgctgg
     2281 ttgctggtac tgctggtggc gtggctgctg tggcggaaag cggtacgccc gcagttaaca
     2341 cgtcgcgccg aggcgatgaa agctgtacag caacaggcgc aggcccgtga ggaagtggaa
     2401 gatgcggtgg aagtccgcct gagcaaagac gaacaactcc aacaacgacg cgctaaccaa
     2461 cgtctggggg cagaagtcat gagccagcgt atccgtgaaa tgtctgataa cgatccgcgc
     2521 gtggtggcgc tggtcattcg ccagtggata aataacgatc atgagtaacc tgacaggcac
     2581 cgataaaagc gtcatcctgc tgatgaccat tggcgaagac cgggcggcag aggtgttcaa
     2641 gcacctctcc cagcgcgaag tgcaaaccct gagcgctgca atggcgaacg tcacgcagat
     2701 ctccaacaag cagctaaccg atgtgctggc ggagtttgag caagaagctg aacagtttgc
     2761 cgcactgaat atcaacgcca acgattatct gcgttcggta ttggtcaaag ctctgggtga
     2821 ggaacgtgcc gccagcctgc tggaagatat tctcgaaact cgcgataccg ccagcggtat
     2881 tgaaacgctc aactttatgg agccgcagag tgccgccgat ctgattcgcg atgagcatcc
     2941 gcaaattatc gccaccattc tggtgcatct gaagcgcgcc caggccgccg atattctggc
     3001 gttgttcgat gaacgtctgc gccacgacgt gatgttgcgt atcgccacct ttggcggcgt
     3061 gcagccagcc gcgctggcgg aactgaccga agtactgaat ggcttgctcg acggtcagaa
     3121 tctcaagcgc agcaaaatgg gcggcgtgag aacggcagcc gaaattatca acctgatgaa
     3181 aactcagcag gaagaagccg ttattaccgc cgtgcgcgaa ttcgacggcg agctggcgca
     3241 aaaaattatc gacgagatgt tcctgttcga aaatctggtg gatgtcgacg atcgcagcat
     3301 tcagcgtctg ttgcaggaag tggattccga atcgctgttg atcgcactga aaggggccga
     3361 gcagccactg cgcgagaaat tcctgcgcaa tatgtcgcag cgtgccgccg atattctgcg
     3421 cgacgatctc gccaaccgtg gtccggtgcg tctgtcgcag gtggaaaacg aacagaaagc
     3481 gattctgctg attgtgcgcc gccttgccga aactggcgag atggtaattg gcagcggcga
     3541 ggatacctat gtctgataat ctgccgtgga aaacctggac gccggacgat ctcgcgccac
     3601 cacaggcaga gtttgtgccc atggtcgagc cggaagaaac catcattgaa gaggccgaac
     3661 ccagccttga gcagcaactg gcgcaactgc aaatgcaggc ccatgagcaa ggttatcagg
     3721 cgggtattgc cgaaggtcgc cagcaaggtc ataagcaggg ctatcaggaa ggactggccc
     3781 aggggctgga gcaaggtctg gcagaggcga agtctcaaca agcgccaatt catgcccgga
     3841 tgcagcaact ggtcagcgaa tttcaaacta cccttgatgc acttgatagt gtgattgctt
     3901 cgcgcctgat gcagatggcg ctggaggcgg cacgtcaggt catcggtcag atgccaacag
     3961 tggataactc ggcactgatc aaacagatcc aacagttgtt gcagcaagaa ccgttattca
     4021 gcggcaaacc gcaactacgc gtgcaccccg atgatctgca acgtgtggat gatatgctcg
     4081 gcgcaacctt aagtttgcat ggctggcgct tgcggggcga tcccacactc catcctggcg
     4141 gctgtaaagt ctccgccgat gaaggcgatc tcgacgccag tgtcgccact cgctggcaag
     4201 aactctgccg tctggcagca ccaggagtgg tgtaatgacc acgcgcctga ctcgctggct
     4261 aaccacgctg gataactttg aagccaaaat ggcgcagttg cctgcggtac gtcgctacgg
     4321 gcgattaact cgcgctaccg ggctggtgct ggaagccacc ggattacaat tgccgctcgg
     4381 cgcaacctgt gtcattgagc gccagaacgg cagcgaaacg cacgaagtag aaagcgaagt
     4441 cgttggcttt aacggtcaac ggctgttttt aatgccgctg gaggaagtcg aaggtgtcct
     4501 gcccggcgcg cgtgtttatg ccaaaaacat ttcggcagaa gggctgcaaa gcggcaagca
     4561 gttgccgctc ggtccggcgt tattaggtcg tgttctggac ggcagcggta aaccgctcga
     4621 tggcctcccc tcccccgata cgacggaaac cggtgcgctg attaccccac catttaaccc
     4681 gttgcaacgt acaccgattg aacatgtgct ggacaccggc gtgcgcccaa tcaatgccct
     4741 gcttaccgtt gggcgtgggc agcgtatggg gctgtttgcc gggtccggcg ttggtaaaag
     4801 tgtgctgctg gggatgatgg cacgttacac ccgcgccgat gtcattgtcg tgggtttgat
     4861 tggtgaacgc ggacgcgaag taaaagattt tattgagaat atcctcggtg ccgaagggcg
     4921 tgcacgctcc gtggtgattg ccgctccggc ggatgtctct ccgcttctgc gaatgcaagg
     4981 tgccgcctat gccacgcgaa ttgccgaaga ttttcgcgat cgtggtcagc atgtgttgct
     5041 gattatggac tccctcaccc gctacgcgat ggcccagcgt gaaattgcgc tggcgattgg
     5101 cgaaccacct gccactaaag gttatccacc gtcggtgttt gccaaattac cggcactggt
     5161 cgagcgtgcc ggaaatggaa ttagcggcgg cggctcgatt accgcgtttt ataccgtgct
     5221 caccgaaggc gatgaccagc aggaccccat tgccgactct gcgcgggcaa tcctcgacgg
     5281 tcacattgtg ctgtctcgcc gactggcgga agccgggcac tatccggcta tcgatattga
     5341 agcgtcgatc agccgcgcaa tgacggcgtt gatcagtgag caacattacg cgcgagtgcg
     5401 caccttcaaa cagctgttgt cgagttttca gcgtaaccgc gatctggtta gcgtcggcgc
     5461 gtatgccaaa ggcagcgatc cgatgctcga caaagccatc gccctgtggc cgcagctgga
     5521 gggctatttg caacaaggca tttttgaacg cgcggactgg gaagcgtctc tccaggggct
     5581 ggagcgtatt ttcccgacag tgtcataacc caggagataa cggcagatgg cagaacatgg
     5641 cgcgctggcg accctgaaag atctggcaga aaaagaggta gaggatgccg cgcgcctgct
     5701 gggtgaaatg cgtcgcgggt gtcagcaggc ggaagaacag ctcaaaatgc tgatcgatta
     5761 tcagaatgaa tatcgcaata acctcaacag cgatatgagt gccgggatga ccagcaaccg
     5821 ctggatcaac tatcagcagt ttatccagac gctggaaaaa gccattactc agcatcgcca
     5881 gcaacttaat cagtggacgc agaaagttga cattgccctg aacagctggc gagaaaagaa
     5941 acaacgtttg caggcctggc agacactgca ggaacggcaa tccacggcgg cactgcttgc
     6001 agaaaaccgc ctcgatcaga aaaagatgga tgagttcgcc cagcgcgccg ccatgaggaa
     6061 acctgaatga ttcgcttagc gcccttaatt accgccgacg ttgataacgc cacattgcct
     6121 ggcggcaaag ccagcgatgc tgcacaagat tttctcgcgt tgttgagcga agcattagca
     6181 ggcgagacaa ctaccgacaa agcggccccc cagttgctgg tggcaacaga taagcccacg
     6241 acaaaaggcg agccgctggt cagcgagatt cttgccgatg cacaacaagc ggatttactg
     6301 atccctgtgg atgaaacacc gcctgtcatc aacgacgaac aatccacatc aacaccatta
     6361 accaccgctc aaacgatgac gttggctgcg gtggctgaca acaatacggc aaaagacgaa
     6421 aaagcggatg atctgaatga agacgtcacc gcaagcctga gcgccctttt tgcgatgttg
     6481 ccgggttttg acaatacgcc caaagtgact gatgtgccgt caaccgtgtt accggcagag
     6541 aaaccaacgc tcttcacaaa actgacttct gcgcaactca caacagcaca gcctgatgat
     6601 gcccccggca cgccagctca gccattaaca ccgctggtag cagaagccca gagtaaagcg
     6661 gaagtcatca gcacaccttc accggtgacc gctgccgcca gcccgctaat cactccacac
     6721 cagacacagc cactgcccac cgtcgccgtg cctgtgttga gtgcaccgct gggttctcac
     6781 gaatggcaac aatcattaag ccagcatatt tcgctgttca cccgccagag gcaacaaagt
     6841 gcagagttgc gtctgcaccc gcaggattta ggtgaagtgc aaatctccct caaagtggat
     6901 gataaccagg cacaaatcca gatggtttca ccgcatcagc atgtacgcgc cgcgctggaa
     6961 gcagcgcttc cggtactgcg tacgcagctg gccgaaagtg gcattcagtt agggcaaagc
     7021 aacatcagtg gcgaaagctt tagtggtcag cagcaggccg cttcccaaca acagcaaagc
     7081 caacgcacag taaaccatga acctctggcg ggggaagacg acgatacgct tctggttccc
     7141 gtctctatac aagggcgcgt aacaggcaac agcggcgttg atattttcgc ctaacgtcag
     7201 aggtagcacc gtaatccgcg tcttttcccc gctttgttgc gctcaagacg caggataatt
     7261 agccgataag cagtagcgac acaggaagac cgcaacacat gactgattac gcgataagca
     7321 agaaaagcaa gcgatcgctt tggatcccga ttctggtatt cattaccctc gcggcctgtg
     7381 ccagcgcagg ttacagctac tggcattcgc atcaggttgc cgctgacgac aaagcgcagc
     7441 aacgcgtcgt accctccccg gtcttctacg cgctggatac cttcacggtc aatctgggcg
     7501 atgcggatcg cgtgctttat atcggcataa ccctgcgcct gaaagatgaa gctacccgct
     7561 cgcggctgag tgagtatttg ccggaagtcc gtagccgttt gctgttactg ttttcgcgtc
     7621 aggatgctgc cgtactggcg acagaagaag gcaagaaaaa cctgattgcc gagattaaaa
     7681 ccacgctttc caccccgctt gttgccgggc aaccgaaaca ggatgtcacc gacgtgctgt
     7741 ataccgcttt tattctgcga taacgacatg ggcgatagta ttctttctca agctgaaatt
     7801 gatgcgctgt tgaatggtga cagcgaagtc aaagacgaac cgacagccag tgttagcggc
     7861 gaaagtgaca ttcgtccgta cgatccgaat acccaacgac gggttgtgcg cgaacgtttg
     7921 caggcgctgg aaatcattaa tgagcgcttt gcccgccatt ttcgtatggg gctgttcaac
     7981 ctgctgcgtc gtagcccgga tataaccgtc ggggccatcc gcattcagcc gtaccatgaa
     8041 tttgcccgca acctgccggt gccgaccaac ctgaacctta tccatctgaa accgctgcgc
     8101 ggcacagggc tggtggtgtt ttcaccgagt ctggtgttta tcgccgtgga taacctgttt
     8161 ggcggcgatg gacgcttccc gaccaaagtg gaaggtcgcg agtttaccca taccgaacag
     8221 cgcgtcatca accgcatgtt aaagctggcg cttgaaggct atagcgacgc ctggaaggcg
     8281 attaatccgc tggaagttga gtacgtgcgt tcggaaatgc aggtgaaatt taccaatatc
     8341 accacctcgc cgaacgacat tgtggttaac acgccgttcc atgtggagat tggcaacctg
     8401 accggcgaat ttaatatctg cctgcctttc agcatgatcg agccgctacg ggaattgttg
     8461 gttaacccgc cgctggaaaa ctcgcgtaat gaagatcaga actggcgcga taacctggtg
     8521 cgccaggtgc agcattcaca gctggagctg gtcgccaact ttgccgatat ctcgctacgc
     8581 ctgtcgcaga ttttaaaact gaaacccggt gacgtcctgc cgatagaaaa acccgaccgc
     8641 atcatcgccc atgttgacgg cgtcccggtg ctgaccagtc agtacggcac tctcaacggt
     8701 cagtatgcgt tacggataga acatttgatt aacccgattt taaattctct gaacgaggaa
     8761 cagcccaaat gagtgacatg aataatccgg ccgatgacaa caacggcgca atggacgatc
     8821 tgtgggctga agctttgagc gaacaaaaat caaccagcag caaaagcgct gccgacgcgg
     8881 tgttccagca atttggcggt ggtgatgtca gcggaacgtt gcaggatatc gacctgatta
     8941 tggatattcc ggtcaagctg accgtcgagc tgggccgtac gcggatgacc atcaaagagc
     9001 tgttgcgtct gacgcaaggg tccgtcgtgg cgctggacgg tctggcgggc gaaccactgg
     9061 atattctgat caacggttat ttaattgccc agggcgaagt ggtggtcgtt gccgataaat
     9121 atggcgtgcg gatcaccgat atcattactc cgtctgagcg aatgcgccgc ctgagccgtt
     9181 agtgatgaat aaccacgcta ctgtgcaatc ttccgcgccg gtttctgctg cgccactgct
     9241 gcaggtgagc ggcgcactca tcgccattat tgccctgatc ctcgctgctg cctggctggt
     9301 aaaacggttg ggatttgtcc ctaaacgcac tggcgttaac ggtctgaaaa ttagcgccag
     9361 tgcttcgctg ggcgcgcgtg aacgggttgt ggtggtcgat gtggaagatg cacggctggt
     9421 gctcggcgtt accgcagggc aaatcaatct gctgcataaa cttccccctt cagcaccaac
     9481 ggaagagata ccgcagcccg attttcagtc ggttatgaaa aacttgctta agcgtagcgg
     9541 gagatcctga tgcgtcgttt gttgtctgtc gcacctgtcc ttctctggct ggttacgccc
     9601 ctcgccttcg cgcaactgcc gggtatcacc agccagccgc tgcctggcgg tggacaaagc
     9661 tggtcgctcc cggtgcaaac gctggtgttc atcacctcgt tgacgtttat tccggcaatt
     9721 ttactgatga tgaccagttt cacccgcatc atcattgttt ttggtttatt gcgtaacgcg
     9781 ttgggaacac cctccgcgcc acctaaccag gtattgctgg gactggcgct gtttttgacc
     9841 ttttttatta tgtcgccggt gatcgataaa atttatgtcg atgcgtacca gccattcagc
     9901 gaagagaaaa tctcaatgca ggaggcgctg gaaaaagggg ctcagccgct gcgtgagttt
     9961 atgctgcgtc agacccgcga ggcggattta gggttgtttg ccagactggc gaatactggc
    10021 ccgttgcagg gacctgaagc tgtgccgatg cgcattttgc tcccggccta cgtgaccagc
    10081 gagttgaaaa ccgcatttca gataggcttc acgattttca ttcctttttt gattatcgac
    10141 ctggtgattg ccagcgtgtt gatggcattg gggatgatga tggttccccc agccacaatt
    10201 gctctgccct ttaaactgat gctgtttgta ctggtggatg gctggcaatt gctggtcggt
    10261 tcgctggcgc agagctttta cagctagaga ggcaaaatga cacctgaatc ggtcatgatg
    10321 atggggactg aagcgatgaa agtcgcgctg gcactggctg ccccactgtt gctggtggcg
    10381 ctggtcacgg gtcttatcat cagtattttg caggccgcca cccagattaa cgaaatgacg
    10441 ctgtcgttta ttccgaaaat catcgccgta tttatcgcca ttattattgc cggaccgtgg
    10501 atgctcaatc tgttgctgga ttacgtccgc accttgttca ctaacctgcc gtatatcatc
    10561 gggtaggccg tacaatgatg caggtaacaa gcgatcaatg gctttcctgg ttaagcctgt
    10621 acttctggcc gttactgcgc gtgctggcgc tcatttctac cgcaccaatt ctgagcgaac
    10681 gcagcgtgcc gaaacgggta aaactgggtc tggcaatgat gatcacgttc gctattgccc
    10741 catcattacc tgccaacgat gttcctgttt tttcgttctt tgctctgtgg ctggccgtgc
    10801 agcagatcct gatcggcatt gcgcttggtt ttaccatgca atttgccttt gccgctgtgc
    10861 gaaccgctgg tgaaattatc ggtctgcaaa tggggctgtc atttgcgacg tttgtcgatc
    10921 cggccagcca tctcaatatg cccgttttag cgcgtatcat ggatatgctg gcgttactgc
    10981 tgttcctgac atttaacggt catttatggt tgatttcact gctggccgat acctttcaca
    11041 ccctgccaat tggtggcgaa ccgttgaaca gcaatgcgtt tctggcactc accaaagcag
    11101 ggagtttgat tttccttaac gggctgatgc tggcattacc gctcattact ctgctgctga
    11161 ccctgaatct ggcattaggc ttacttaatc gtatggcccc gcaattatcc atttttgtta
    11221 ttggatttcc attaattctg actgtcggta tctctttaat ggcggcatta atgccgttaa
    11281 ttgcgccttt ttgcgaacat ttattcagtg agatgtttaa tttgctggct gatattatta
    11341 gtgaattgcc attaatataa ttccgtaacg tttatcatgt tatcctaagg attatccgaa
    11401 aaataatacc tacgaacatc ttccaggata ctcctgcggc gaaatatttg ttttaagctc
    11461 actcacatat cgcaacattt actttacttt aagacaattc caggcaaatt atacaacact
    11521 ttacgggata gtaagtccgc ctgaaaaatc gcgagagtgg cgcattaggt gacccatgtt
    11581 gttccgttta gtcatgatga aatattcagg taaggggaat tatcgttacg cattgagtga
    11641 gggtatgcca tgtcaacgat tattatggat ttatgtagtt acacccgact aggtttaacc
    11701 gggtatctgt tgagtagagg ggttaaaaaa agagaaatca acgacattga aaccgttgat
    11761 gaccttgcca tagcttgtga ttcacagcgc ccttcagtgg tgtttattaa tgaggactgt
    11821 ttcatccacg atgcttctaa cagtcagcgt atcaagctca tcattaatca acatcccaat
    11881 acgttattta tcgtttttat ggcaattgcc aatgttcatt ttgatgaata tctattggtc
    11941 agaaaaaatt tattgatcag ttctaaatcg attaaaccgg aatctctcga cgatatcctt
    12001 ggcgatattc tgaaaaaaga gacaacgata acctcgtttt taaatatgcc gacgttatca
    12061 ttgagccgaa ccgaatcgag tatgttgcga atgtggatgg caggtcaggg aaccattcaa
    12121 atctctgacc aaatgaatat caaagccaag accgtttcat cgcataaagg taatattaaa
    12181 cgtaagatca aaacgcataa taaacaggtt atctaccatg tcgtccgact gacggataat
    12241 gtgactaatg gtatttttgt caacatgcgc taacacattc tgactggtgg tttcccacca
    12301 gtcaggccga attagattac tctgctttct ccacaaagat accgtcctga tgccctgctt
    12361 cattaaagaa ccagatgccg agcgggtagt cttccagcga aaccaggtac attgtgcctt
    12421 cactaaactc ctcaactgcc agtaccacgc caggacgacg cggaccgcca tccgttttga
    12481 ctgttacccg atcattcacc ttcatcgttt tcctcctgtg gctttgtgcc agtgtagaac
    12541 aatttcgttt ttctggcagc gccaggcgcg cgcgagtgct gattttctcg acggtctata
    12601 cttaagagat gccaggcgga cttaacgact ggccgcaaca acagagtaac ggttgcgagg
    12661 aaagatgatg aaaaccgcaa aagagtacag cgataccgca aaacgtgagg tcagcgtcga
    12721 tgtcgatgcc ctgctggcgg cgatcaatga aattagcgaa agcgaagttc atcgcagcca
    12781 gaacgattct gaacacgtta gcgtcgatgg acgtgaatat catacatggc gtgaattggc
    12841 ggatgccttc gaactggata ttcatgactt cagcgtctct gaagtgaatc gttgaatgca
    12901 caataaaaaa atcccgaccc tgagggggtc gggatgaaac ttgcttaagc aagaagcact
    12961 taaaaaattc gttacaccag gaaatctgat gtgttcatca ccttatccgc aatttttttc
    13021 gctgacaaga aaatattcgt gatatgaatg attaacgcta cttatgaata gaaatgtgac
    13081 gcgcgtcacc ccttggtggc tctggcgtca acgggatcgc tcccgaaaaa atcatctttg
    13141 ataatttgtg ctgttttgag aatcatcctg gtaaggggag tactgcgcaa ccatgctttc
    13201 aattcaacaa ccactactgg tttttagcga tcttgatggc accctgctgg acagtcatag
    13261 ttatgactgg caaccagctg ccccctggct cagccgttta cgcgaagcga atgttcccgt
    13321 cattctctgt agcagtaaga catcagccga aatgctgtac ttgcaaaaaa cgttggggct
    13381 acaaggttta ccgctgattg ccgaaaatgg cgcagtgatc cagcttgctg agcaatggca
    13441 ggatatagac ggttttccac gcatcatctc aggtattagc catggcgaaa tcagccaggt
    13501 tttaaatacg ctacgcgaga aagaacattt taaattcaca acttttgatg atgtcgacga
    13561 tgcaaccatc gccgaatgga cgggattaag ccgtagccag gcggcgctga cgcagctaca
    13621 tgaggcgtcg gtaacgctaa tctggcgcga cagtgacgag cgtatggcac aatttaccgc
    13681 tcgtctgaac gaactgggct tgcagtttat gcagggtgcg cgcttctggc acgtcctgga
    13741 tgcctctgcc ggaaaagatc aggctgccaa ctggattatc gcgacctatc aacaatcgtc
    13801 aggcaaacgc ccaaccacac ttggcctggg cgatgggcca aacgatgcgc ccttactgga
    13861 ggtaatggat tacgcggtga ttgtgaaagg gttaaaccgt gaaggggtgc atctgcatga
    13921 tgaggatccg gcccgcgtct ggcgaacgca gcgtgaagga ccggaaggct ggcgtgaagg
    13981 gctggaccat tttttctccg cccattaagc gttatcgctc gcgaataccc gattgcgccc
    14041 ggcctgttta gcgagataaa gtcgacggtc ggccagtgac tggagttgtt caaaatcata
    14101 atcaccggtt tcctcgctgc tgcttacccc cagcgaggca ctgatgcgta tcgtcgtact
    14161 cttggcgatc aacatctctt tttcatttag cttcaggcga atacgttctg caacctccgc
    14221 agcctgcgtc aggctcgcgc ctggcagaat cacacaaaac tcctcaccac cgacccgccc
    14281 ggcaacatcc tgcgctcgca aggaactgct aattaatccg gcagcatgag aaagaacacg
    14341 gtcgcccgcc tgatgaccaa agcggtcatt aatcgcttta aaatggtcaa ggtcgacctg
    14401 aatgacagaa aaaggatgtt ggtgcgtctg acacaattta gcgagcggac gggctttttc
    14461 gaacagtgcg ccacggttat ataaacgcgt taaggtgtcg tgccacgcct gccagtgcaa
    14521 cgagctttgc aaaacataca tgttgctgac catccggcga atcacatacc aggagatgag
    14581 taacatggtg gtaaagagcg cccacagcag ggttaatgca atgctgatac tgccgaaatc
    14641 gccgcgcacg ccttcgctta gcgtatggac gcgcaccagc acaccgtcga aatggtccag
    14701 acgttcccag ctaacatagc gactgttcat acgaatgccg ccccgcgtgt catgttccat
    14761 tgcctgcgcc agcaaggcca gttcacgagg atcaaaaata ttccctgttg gatggtcagg
    14821 attggaagag gtcaataatc tcagcttact gtcatagagc tgatactcac catcgaggtt
    14881 tttatcgatg gcgtttctta aaaattgctg catggtacgc acgggaatac tcatccccag
    14941 cacgccatac cagtagtttt cactgtctac cggaacactg acggtaacct gcggttcagt
    15001 attgctggcg tgttccggtt gcgaagtgaa ccagcgtacc gcgcggtgac gattttctcg
    15061 ttgcgaatgg ccgacaaacc agggttgggt gacatagccg taataacgcg ttggtacatt
    15121 gcgcgtaaac aaggtcggct gcgtcgaaac gtaaaatccg gcacgcgaga catacatcgc
    15181 ctgttcaacc atcgacgagg agttgtgtgc cagtcgcagc aagtaaccaa cttccagtgc
    15241 agcggtaatt tcattgtcga ggctttcatt ttcgcgagac aggagattcc cctcgctgac
    15301 taatgcatcc gacacaccat tgactggcag ggtgcgtcgt cggttgagtt cgatttgcca
    15361 ggcgtgctcg tcgcgatgct gttcgaactc ggttacggca ttacgcagag aggtgaaatc
    15421 cagtggcgct acgagagctt cgcgcatgcc attacgcaaa aagatcagtt tgtcgacgtt
    15481 atactgcaaa tgcttatcga gcgcgttggc tacgttttcc agatgattac gctggctgga
    15541 gatataggca tcttccagca ccaccacttc acgccaggtg agcaaggtgg aaaaaagcaa
    15601 taccacgata aagcagagat taacgacatg accaggcccc aggcggcgtg cgagtttttt
    15661 caaccagctc tggttttcca tttttgtctc gtgctgcacc ctgacgactc ccaacctgct
    15721 tttttatgat tctggcataa cctggcgata gcgtaccgca aaacagcgat aattttataa
    15781 tggtggcatg ataagacgcg acagacatcg atagttttat aacgtaggca tgataagacg
    15841 cgatagcgtc gcatcaggca attcagtgca gacactggca cacccgaccg gatgcgacgt
    15901 gagcgccaca tccggtcggg tgttaatgat accctttcaa acttcttccg cagaacgact
    15961 cccctccccc ggaacaagct cggtttcatg aaaagcttcc cgcttgaccc cgtaaccgtc
    16021 ataccaacga cactcaacca taccgctgga gtatccggtg acaatcatcc gcgggccgcc
    16081 ctctttaacc gtaacttcct cactaaccat aaagctcata ctcgcctcct tttttcgagt
    16141 gaaacttgtt caccttagtt gaagatggcg aattttgcat aatagcctgt gcgagtatta
    16201 atgtgcctga ccgcgtattt tcgctacggc tttcactccc agcaccacga tgccgccgat
    16261 gataaatcca agaatcagat ttaaaacagt cggtaatatc atcgccacca ctgcactttg
    16321 ctgcccggcg aaatgttcaa tggcgtgatg cagcggcgca attccatgta ccacaatccc
    16381 accgccgacg aggaacatcg ccagcgtgcc gacaatcgat aacgccttca tcaaccaggg
    16441 cgcaatgatc aataaccctt tgcctaatgc ctgcatcagc gcgctggatt tttccgccag
    16501 ccaatacccc aggtcatcaa tcttaacgat aacccctacc agaccgtaga cgcccacggt
    16561 cactaccagc gcgatgcctg aaagcaccag cacctgatta agcaacggcg cttcggccac
    16621 aatccccagc gtgatggcga cgatttccgc agacaatata aaatcggtac gaatcgcccc
    16681 ttttattttg tctttttcaa acttcagcgg atcctgcgcc gccagcttct ccagacgctg
    16741 ctgactctgc gccggatctt ctttatgttt acgcgcctcc agcatatgca gcactttctc
    16801 taccccttca aagcagagaa acgcgccgcc aatcatcaac agtggcgtaa tcgcccacgg
    16861 gataaacgca ctgatgatca gcgccagcgg caccagaatc actttattga tcagcgatcc
    16921 tttcgccacg ccccagacca cgggaagttc ccggttggcg cgcacgcctg caacttgttg
    16981 cgcattgagc gataagtcat cccctaatac accggcggtt ttcttcgccg ccagtttgcc
    17041 catcacggag atatcgtcca gcagtgtggc gatatcatcg agcaacgtca gtaaactact
    17101 cccggccaaa agcactccct ctcattttta ttgttgaagg gtgtaagtat gaagcaaaaa
    17161 cggcagctct gatacttgct tcacaggtca acatttattt aacaatcaag tgcaattata
    17221 tctctcgcca gcagaatgat tttcacgttt actatgaacc acctttttat ttcagccgtg
    17281 agggattatg cgtttccgcc agttgttacc gctttttggc gcgctgtttg cgttgtatat
    17341 catttggggc tcaacctatt ttgtcattcg gattggcgtg gaaagctggc ctccgttaat
    17401 gatggcgggc gttcgattcc tggcagccgg tattttattg ctggcatttt tgctactgcg
    17461 cggacacaaa cttcccccgc tacgtccgct gctcaatgcc gcgctgattg gcctgttatt
    17521 gctggctgtc ggtaatggca tggtgacggt tgccgaacat caaaatgttc cttccggcat
    17581 cgccgccgtt gtggttgcaa ccgtgcccct ctttaccctg tgcttcagcc gcctgtttgg
    17641 cattaaaacg cgcaaactgg aatgggtggg tatcgccatt gggcttgccg gaatcatcat
    17701 gctcaatagc ggtggaaatt taagcggcaa tccgtggggc gcgattctga ttttaatcgg
    17761 ctcgattagc tgggcgtttg gctcggttta tggctcgcgc attaccttac ctgtagggat
    17821 gatggcgggt gcgattgaga tgctggcggc tggcgtggtg ttaatgattg cgtcgatgat
    17881 tgcgggtgaa aaactgacgg cgctcccttc cctttcaggc ttccttgcgg tcggctatct
    17941 ggcgctgttt ggttcgatta tcgccattaa cgcttatatg tatttaatcc gcaacgtcag
    18001 tccggctctc gccaccagct acgcctacgt taacccagtg gtcgcggtct tgctgggtac
    18061 ggggctgggt ggcgaaacgc tgtcgaagat tgaatggctg gcgctcggcg taattgtttt
    18121 cgcggtggta ctggtcacgt tgggaaaata tctcttcccg gccaaacccg ttgtcgcgcc
    18181 agttattccg gatgcatcaa gcgagtaaat gaatcccctg cgtgtcgatc tgcgcgctgg
    18241 caccttcgcc gcagatccac tcttccagac gctcggtaag cgccgcatcc gtcagcttct
    18301 cgcgcccgcg taacgcacat tcccagacaa tcaatacgcg ccaaccgagt tcctgcaaat
    18361 ggctgatatc acgacgatcg cgctcgacat ttttacctat cttctccagc caaaaatctg
    18421 ttcgcgtggc ggggacttta aacaggtagc aatggtgatg gtgccagaag caaccgtggg
    18481 caaatatcac acagcgaaat tcatcaagga caaaatccgg gcgtccgggc agactggcgt
    18541 cctgaacgcg aaacgccagg ccctgcccag ttaacaaact ggcgaggcgc ttctcaatcg
    18601 ccgtatcacg cgtggcaatc gcgcgcatat ttttgctgcg ggtggcctta tcgtgaacgt
    18661 cgaccatgtt gtgcctcttg ctgacgcaac gccaccgcct gtttgatttt tggctcaagc
    18721 agttttgcca ccgcggcaaa gaccggcacg accaccgagt taccgaactg gcgataagcc
    18781 tgagtgtccg aaaccggaat gcggaatttc gcttctcccg gcgcttcaaa acccattaag
    18841 cgcgcacatt cccgaggcgt taaccgacgt gggcgatgtt gctgattcag cggatcgtca
    18901 aagtctttct cacccgttgc catatcccag ccgcgatcga ttaaaatttc cgcgccatct
    18961 ttgtaataac gcgcagacag cgtacgcgtg acgctttgcg gattgttcgg ataaaccatt
    19021 ccataaccga agccgttacc gcgcgcctga tgttttttcg catatcgata gaggtacttc
    19081 cacagtaccg gcgtcaggat atatttcgcc tcgaccatcg ggtccagcag ctgcgccagc
    19141 gtcactcgct gtgcagggaa acatttgctg atatcacgca gagtaaaatc ggctttcaga
    19201 ttcagatcgc gacgaaaacc caccagcacg atgcgttcac ggtgctgcgg cagaaaatgt
    19261 ttgccgtcga tgattttcgg atcgtccggc ccgttatctt ctgcatcagc cacgtcatag
    19321 cccagttcgt ccagcgtctg catgatgatg cggaacgttt taccctggtc gtgacttttc
    19381 aggtttttga cgttttcgag cacaaacatc gccggacgac gcgcgtcgat aatgcgcacc
    19441 acatcaaaga acagcgtgcc ctgggtatcg caggcaaaac cgtgcgcccg cccgagcgag
    19501 ttctttttcg atacgccagc cagcgaaaat ggctgacaag ggaaaccggc cagtaaaaca
    19561 tcgtgttcag gaatgtgttg acgaatatgt tccgccgccg cctcatcact cacgccttct
    19621 tgatggctga gggtgatgtc gcggatatcc tcattaaaat gatgcgtcgc cgggtcgcaa
    19681 taatggttgg ctttataagt gcgtaccgca tgtttgttcc attcgctggt aaacacgcac
    19741 tgtccaccaa tcgattcaaa accgcgacgg atgccgccaa ttccggcaaa tagatcgata
    19801 aagcgaaacg cataatgcgg atgatgtgcc ggtggtttgg gtaacaacgt ttgcagatgg
    19861 gcgaactctt tctcacttaa acggtgccat gccgagtcat tcgccagcgc acgttttaaa
    19921 attgccgcgc tccagtgatt ctcacccaca ccattaagct gcgccaccag cgttttaaca
    19981 tcataaattt gcagcagttt ctccagcatt gcctgtgcgg cattcccggt gctgtatgaa
    20041 tcggttactg atatattttc ctgcatagat ttaaccggcc atctgagatg gccgacaggt
    20101 taacataatt actgccgttt agggccacgc gctcagccag caggtgaaaa cgcatctatc
    20161 accttgtgat cgataccttc attctccccc gccagttcgg caccgagctt cgccataaac
    20221 tcgactaaaa attgcgcgtt gtgctgtgcc agctgcttgc ctcttgcggt ttgcatggtt
    20281 tgcggtaatt tcagtaactt tgtctggaaa tgatccagcg cgtagcgttt atcatcaagc
    20341 gggcgatgct gtgcgaacgg atcttcgcca tcaaacagcg ccaagcccaa tgcccctgaa
    20401 acggcaaata cgcgcgccag gccaatcgcc cccagggctt ccagccgatc cgcatcctgg
    20461 acgattttag cttccgttgt taagggggcg atttgcgcgc tgaaactgtg agcggcaatg
    20521 gcatgacaaa cggcctcaat tttctccgcc ggaaattgca cgaactctt
//
LOCUS       DAZFWL020000081        20380 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFWL020000081 DAZFWL020000000
VERSION     DAZFWL020000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.081"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(152..898)
                     /gene="cutC"
                     /locus_tag="ACHKNP_RS15545"
                     /old_locus_tag="pgaptmp_003109"
     CDS             complement(152..898)
                     /gene="cutC"
                     /locus_tag="ACHKNP_RS15545"
                     /old_locus_tag="pgaptmp_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGU4705235.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(912..1478)
                     /gene="yecM"
                     /locus_tag="ACHKNP_RS15550"
                     /old_locus_tag="pgaptmp_003110"
     CDS             complement(912..1478)
                     /gene="yecM"
                     /locus_tag="ACHKNP_RS15550"
                     /old_locus_tag="pgaptmp_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4705236.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1694..3427
                     /gene="argS"
                     /locus_tag="ACHKNP_RS15555"
                     /old_locus_tag="pgaptmp_003111"
     CDS             1694..3427
                     /gene="argS"
                     /locus_tag="ACHKNP_RS15555"
                     /old_locus_tag="pgaptmp_003111"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGU4705237.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3469..3513)
                     /gene="yecV"
                     /locus_tag="ACHKNP_RS15560"
                     /old_locus_tag="pgaptmp_003112"
     CDS             complement(3469..3513)
                     /gene="yecV"
                     /locus_tag="ACHKNP_RS15560"
                     /old_locus_tag="pgaptmp_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HGU4705238.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3604..4092
                     /gene="yecT"
                     /locus_tag="ACHKNP_RS15565"
                     /old_locus_tag="pgaptmp_003113"
     CDS             3604..4092
                     /gene="yecT"
                     /locus_tag="ACHKNP_RS15565"
                     /old_locus_tag="pgaptmp_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HGU4705239.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4212..4604)
                     /gene="flhe"
                     /locus_tag="ACHKNP_RS15570"
                     /old_locus_tag="pgaptmp_003114"
     CDS             complement(4212..4604)
                     /gene="flhe"
                     /locus_tag="ACHKNP_RS15570"
                     /old_locus_tag="pgaptmp_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HGU4705240.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4604..6682)
                     /gene="flhA"
                     /locus_tag="ACHKNP_RS15575"
                     /old_locus_tag="pgaptmp_003115"
     CDS             complement(4604..6682)
                     /gene="flhA"
                     /locus_tag="ACHKNP_RS15575"
                     /old_locus_tag="pgaptmp_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HGU4705241.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMELQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHAPRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6675..7823)
                     /gene="flhB"
                     /locus_tag="ACHKNP_RS15580"
                     /old_locus_tag="pgaptmp_003116"
     CDS             complement(6675..7823)
                     /gene="flhB"
                     /locus_tag="ACHKNP_RS15580"
                     /old_locus_tag="pgaptmp_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HGU4705242.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8025..8669)
                     /gene="cheZ"
                     /locus_tag="ACHKNP_RS15585"
                     /old_locus_tag="pgaptmp_003117"
     CDS             complement(8025..8669)
                     /gene="cheZ"
                     /locus_tag="ACHKNP_RS15585"
                     /old_locus_tag="pgaptmp_003117"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HGU4705243.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8680..9069)
                     /gene="cheY"
                     /locus_tag="ACHKNP_RS15590"
                     /old_locus_tag="pgaptmp_003118"
     CDS             complement(8680..9069)
                     /gene="cheY"
                     /locus_tag="ACHKNP_RS15590"
                     /old_locus_tag="pgaptmp_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HGU4705244.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9084..10133)
                     /gene="cheB"
                     /locus_tag="ACHKNP_RS15595"
                     /old_locus_tag="pgaptmp_003119"
     CDS             complement(9084..10133)
                     /gene="cheB"
                     /locus_tag="ACHKNP_RS15595"
                     /old_locus_tag="pgaptmp_003119"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HGU4705245.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10136..10996)
                     /gene="cheR"
                     /locus_tag="ACHKNP_RS15600"
                     /old_locus_tag="pgaptmp_003120"
     CDS             complement(10136..10996)
                     /gene="cheR"
                     /locus_tag="ACHKNP_RS15600"
                     /old_locus_tag="pgaptmp_003120"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HGU4705246.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRCFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11015..12619)
                     /gene="tap"
                     /locus_tag="ACHKNP_RS15605"
                     /old_locus_tag="pgaptmp_003121"
     CDS             complement(11015..12619)
                     /gene="tap"
                     /locus_tag="ACHKNP_RS15605"
                     /old_locus_tag="pgaptmp_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HGU4705247.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNNDLEHQATWLESNQLSDFLTAPVQESQNAF
                     DVNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQP
                     LAIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHI
                     GIAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTA
                     QAGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVD
                     DIMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSR
                     VAVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12665..14326)
                     /gene="tar"
                     /locus_tag="ACHKNP_RS15610"
                     /old_locus_tag="pgaptmp_003122"
     CDS             complement(12665..14326)
                     /gene="tar"
                     /locus_tag="ACHKNP_RS15610"
                     /old_locus_tag="pgaptmp_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HGU4705248.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQAPNWETF"
     gene            complement(14471..14974)
                     /gene="cheW"
                     /locus_tag="ACHKNP_RS15615"
                     /old_locus_tag="pgaptmp_003123"
     CDS             complement(14471..14974)
                     /gene="cheW"
                     /locus_tag="ACHKNP_RS15615"
                     /old_locus_tag="pgaptmp_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGU4705249.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14995..16959)
                     /gene="cheA"
                     /locus_tag="ACHKNP_RS15620"
                     /old_locus_tag="pgaptmp_003124"
     CDS             complement(14995..16959)
                     /gene="cheA"
                     /locus_tag="ACHKNP_RS15620"
                     /old_locus_tag="pgaptmp_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HGU4705250.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16964..17890)
                     /gene="motB"
                     /locus_tag="ACHKNP_RS15625"
                     /old_locus_tag="pgaptmp_003125"
     CDS             complement(16964..17890)
                     /gene="motB"
                     /locus_tag="ACHKNP_RS15625"
                     /old_locus_tag="pgaptmp_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HGU4705251.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17887..18774)
                     /gene="motA"
                     /locus_tag="ACHKNP_RS15630"
                     /old_locus_tag="pgaptmp_003126"
     CDS             complement(17887..18774)
                     /gene="motA"
                     /locus_tag="ACHKNP_RS15630"
                     /old_locus_tag="pgaptmp_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HGU4705252.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQMTTEEA"
     gene            complement(18901..19479)
                     /gene="flhC"
                     /locus_tag="ACHKNP_RS15635"
                     /old_locus_tag="pgaptmp_003127"
     CDS             complement(18901..19479)
                     /gene="flhC"
                     /locus_tag="ACHKNP_RS15635"
                     /old_locus_tag="pgaptmp_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HGU4705253.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19482..19832)
                     /gene="flhD"
                     /locus_tag="ACHKNP_RS15640"
                     /old_locus_tag="pgaptmp_003128"
     CDS             complement(19482..19832)
                     /gene="flhD"
                     /locus_tag="ACHKNP_RS15640"
                     /old_locus_tag="pgaptmp_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HGU4705254.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4908 a         5597 c         4951 g         4924 t
ORIGIN      
        1 cacaacgcta tgttcactgt cacccgctca acagatcacg gttatcgttc gttttttata
       61 ctgctcaggg ataaaaaaag gcccctgttg aaattgcagg ggcctggtac gagcaagcat
      121 catattgggc gacatgatgc aacggtaaaa atcatttggc ctgatggcgt tcaatgattc
      181 ctttcatttc agcaaccgcc gccccgtcta cgatataacg cgaatactcg tccgcgtgtt
      241 catctgatga catggacaat ccttgattac gataacgcat cggtgaggct tgccacgctc
      301 ccgcggagct atggacttcc agcactccgg catcgaggaa gtggtgcagg ttttctgcac
      361 ggactcctgc tccggccata atgattggag catcacgatg ggcaataagt tccataattt
      421 ttgataaacc ttgcagtgcg tctgattttt gccctgatgt cagtacccgg gcaatgccca
      481 attccgcaag attattgagt gtatataaag ggttagcgca catatcgaag gcgcgatgaa
      541 aagtcactgc cagcggaccg gcagcagcca ttattttttc cattcgtggc atatcgacat
      601 tcccgtcaac atcgagaacg cccgtcacca gtccaggaaa acctaattcg cggaccgtgc
      661 gcacatcctc aagaatggcg gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg
      721 ggcgaattat cggatgcaca gggatcgtca cccgctggcg cacggatttc agtaccccca
      781 acgacggcgt taagcccccc tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt
      841 tttgctgcgc cgttagtgca cattccatgc tgtaacagca aatttccagt aatgccattt
      901 ttgctcctta attacgctga ctgctcgctg gcaacgatct cttcaatgga ccacggatga
      961 aacttaatag ttgttttgcc atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc
     1021 tcaccttttg gtgaactggt tttaactgag atccccggta ggctgagtcc ttcatcagaa
     1081 agcaatgcca gcgcacgggc attcagcgtt tctggatcac ccggcagaac gatttcaata
     1141 tgttcccaac cttcgtgtgg gtaacgtttt tccccgggcc acggtagctc cacaatagaa
     1201 aactgccagt gcgcaacctg taccggttca tgcaatttaa acagacaaat cggtctgcca
     1261 ttgatcatat tttctgacaa aagctcgcca cactgttcaa acccgcgacg ccagcgttca
     1321 gcagtggcgt tttgatggca acgcaaagaa atgtgatcgg cagtcagcgg agtgatattc
     1381 aaccccagac ggagggaaag ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca
     1441 atatcctgca gctcgtcgat agattgccag ttcgccataa tcactcttcg tctttcagta
     1501 aaagcgttaa tttaccctgt tgccctgcgc caaccaaccg ctgatttcac gccgcttctg
     1561 atgcaatagt gaaaacggca atacgccacg cgcacgttgc tgacgaaaac agccatttgc
     1621 agtatactcc cgccctaatt tctttaactg gtgcgggcaa tttttgctcg cttcatcaat
     1681 gtaaggtatt ccggtgaata ttcaggctct tctctcagaa aaagtccgtc aggccatgat
     1741 tgcggcaggc gcgcctgcgg attgcgaacc gcaggttcgt cagtcagcaa aagttcagtt
     1801 cggcgactat caggctaacg gcatgatggc agttgctaaa aaactgggta tggcaccgcg
     1861 acaattagca gagcaggtgc tgactcatct ggatcttaac ggtatcgcca gcaaagttga
     1921 gatcgccggt ccaggcttta tcaacatttt ccttgatccg gcattcctgg ctgaacatgt
     1981 tcagcaggcg ctggcgtccg atcgtctcgg tgtttctacg ccagaaaaac agaccattgt
     2041 ggttgactac tctgcgccaa acgtggcgaa agagatgcat gtcggtcacc tgcgctctac
     2101 cattattggt gacgcagcag tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc
     2161 aaaccacgtc ggcgactggg gcactcagtt cggtatgctg attgcatggc tggaaaagca
     2221 gcagcaggaa aacgccggtg aaatggagct ggctgacctt gaaggtttct accgcgatgc
     2281 gaaaaagcat tacgacgaag atgaagagtt cgccgagcgt gcacgtaact acgtggtaaa
     2341 actgcaaagc ggtgacgaat atttccgcga gatgtggcgc aaactggtcg acatcaccat
     2401 gacgcagaac cagatcactt acgatcgtct caacgtgacg ctgacccgtg atgacgtgat
     2461 gggcgaaagc ctctacaacc caatgctgcc aggaattgtg gcggatctca aagccaaagg
     2521 tctggcagta gaaagcgaag gggcgaccgt cgtattcctt gatgagttta aaaacaagga
     2581 aggcgaaccg atgggcgtga tcattcagaa gaaagatggc ggctatctct acaccaccac
     2641 tgatatcgcc tgtgcgaaat atcgttatga atcactgcat gccgatcgcg tgctgtatta
     2701 catcgactcc cgtcagcatc aacacctaat gcaggcatgg gcgatcgtcc gtaaagcagg
     2761 ctatgtaccg gaatccgtac cgctggaaca ccacatgttc ggcatgatgc tgggtaaaga
     2821 cggcaaaccg ttcaaaaccc gcgcgggtgg tacagtgaaa ctggccgatc tgctggatga
     2881 agccctggaa cgtgcacgcc gtctggtggc agaaaagaac ccggatatgc cagctgacga
     2941 gctggaaaaa ctggctaacg cggttggtat tggtgcggtg aaatatgcgg atctctccaa
     3001 aaaccgcacc acggactaca tcttcgactg ggacaacatg ctggcgtttg agggtaatac
     3061 cgcgccatac atgcagtatg cttacacgcg tgtattgtcc gtgttccgta aggcagaaat
     3121 taacgaagag caactggctg cagctccggt aatcatccgt gaagatcgtg aagcgcaact
     3181 ggcagctcgc ctgctgcagt ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc
     3241 gcatgtgatg tgtgcttacc tgtacgatct ggctggtctg ttctctggct tctacgagca
     3301 ctgcccgatc ctcagcgcag aaaacgaaga agtgcgtaac agccgtctga aactggcaca
     3361 actgacagcg aagacgctaa agctgggtct ggatacgctg ggtattgaga cagtagagcg
     3421 tatgtaatcg atttttcgtg agagtaaagc ctgatcaggg ttacctgatc aggctttttt
     3481 attgccatct aaatgtattc tgaaaatgga catgccattg ttttctgact gttggataag
     3541 aggccagaag cgtaatatcc ggccccaggg aaacgataac ggttgaattt aaggaatacc
     3601 gcagtgttta aatttcttgt attaacatta ggcattatct cttgccaggc ttacgcagaa
     3661 gatacggtta tagtaaacga ccatgacatt tcagccatca aagattgttg gcaaaaaaat
     3721 tcagatgatg atactgacat taacgtgatc aaatcatgcc tgcgacaaga atacaatctc
     3781 gtcgatgcgc aattaaataa agcctatggt gaagcttatc gttatataga acaagtgcca
     3841 cgcacaggtg taaaaaaacc tgataccgaa caacttaact tgcttaaaaa atcacagcga
     3901 gcctggctgg attttaggga caaagaatgt gaattaatcc tttcaaatga ggacgttcag
     3961 gatttaagtg acccttattc tgaatcagaa tggctctcat gtatgatcat acagaccaat
     4021 acgcgaactc gccagttgca gctataccgt aactctgaag atttttatcc aagccctttg
     4081 acaagaggat aattcacatc tttttggcat gttttgttgc aagctattcc tgataaataa
     4141 ttgcaacaag acatcgagcc tttttcactg agttattaaa catactcgcg agcgcgtaat
     4201 ttttttgtcc ttcagcgata attcacaatc acttcattac gttgtacctt tagcggtgga
     4261 attaaccgac caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata
     4321 ccggaaaagg ccacggtggt tccgctctgc ccctctaatt cgacacaacg agattgcgaa
     4381 cacaagcgaa cccgcagtcc tgaaggtgtc gggccgataa gctgataacg ccacgctacc
     4441 agcgtcatca atcctgaagc aggttgtcgc gtagaaagag gcgcagacga catcgactca
     4501 ccgcgatgat ttagcgtaat tcctacacta cttgcctgcc acatcccctc cccggcggct
     4561 tgcaccagca gcggaaacaa taatattgct aataaggttc tcattatttg ccgccaattg
     4621 tcgccgtcat gcggatatgt cggttatcag acagttccag attcgacagg accactaact
     4681 gcggcaagct gcggcgcagg aagcgagaca ataatggtcg cggcgcgtgg ttcaccaaca
     4741 atactggcgg cgcacccagc atctcctgac gggatagcgc ttcctgagtt tgcgccagta
     4801 aacgatccgc cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac
     4861 gttccagcgg tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct
     4921 gggtaatcgc ccgtcccaac gccacgcgca cgacggcggt taattcatgt ggatcgcttt
     4981 ggatgggcgc atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt
     5041 tttcatcgag gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa
     5101 cgagatcttc cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct
     5161 cctgacgacc aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta
     5221 ccaccgtgct ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac
     5281 tttcaatcca gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg
     5341 tcccggcagc ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc
     5401 caatctccac gcctttcatc aaaatgcgat agcgggcagg ttgcagctcc atattgtcgc
     5461 gaatgtgcac cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc
     5521 gtatacggcc caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac
     5581 ccacttccat tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg
     5641 tattattctc tgccattttt accggtttgg gttcgacagg cgctttttgt tcgcgtccgc
     5701 gtatccacca ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca
     5761 ttccaggcac caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt
     5821 tactgaaaag ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa
     5881 cgatgacccc cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc
     5941 caatggtcaa tagcgtataa ctttccgccg cgtgtcccat gctcatgcca tgttgcagca
     6001 cgccgaccag caacccgccg acaacgttaa tgaccatgat gaggatcccg gcgatggcat
     6061 cgccgcgaac aaacttactt gccccgtcca ttgagccgta aaaatcggct tcctgagtca
     6121 cttcggagcg gcgttttttc gcctcatctt caccaatcaa tccagcgtta aggtcggcgt
     6181 caatcgccat ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc acttctgcga
     6241 tacgcccggc acctttggta atgaccataa agttgatgat cacgagaatg acaaacacta
     6301 cgataccgat agcgaaattg ccaccaacga ggaagtgacc gaatgcttcg accaccttcc
     6361 cagccgccgc cgcgccggta tgcccttcca tcaaaatgat acgggttgaa gccacgttaa
     6421 gtgccagacg caacaacgta gtaaacaaca gaatggtcgg aaacgcagca aactcaagcg
     6481 tgcgctgggt aaacatcgcc accagcaaca ccatgatcga caaggcaata ttgaaggtaa
     6541 acaacaggtc gagtatgaat gcgggcagtg gcagcaccat catcgacaag atcaacagga
     6601 tcaaaatcgg tccggcaagg atctgccatt gtgtcgattt caggtttgcg ggcaggcgca
     6661 gcatcgcggc cagattactc atgggtcggt ttctcgttaa taaaatccag ggcttccggc
     6721 accggaagat gagtaggttg tacagggcgc tgtccaccag ccagacgcca gcgtttcagt
     6781 tgccagaccc aggccagcac ttccgccacc gcggcgtaca gttgacccgg gatttgttga
     6841 ccaatctccg catgtcgata cagcgctcgc gccagcggcg gcgcttcaag cgtcgggacg
     6901 ttattttcag cgccaatttc acgaatgcgc agcgcgacca gccctgcacc tttagcgacc
     6961 actttcggtg cgctcatttt gttttcgtca tactgcaacg ctaccgaata gtgggtcggg
     7021 ttattgacaa tgacatccgc tttcggcaca tcggccatca tccgacgccg tgcagcagct
     7081 cgctgcatct gacggatccg ccctttaaca tgagggtcac cttcgctttg tttgaactca
     7141 tcacgaatat cctgccgtga catacgcagc tttttcaggt ggctgaatat ttggaaaaag
     7201 acgtcaaatc ccaccattgg aatgacacca agcaccacca gcagtgcgca tagccctacc
     7261 aaatccatcg cattacccat ggcggtaatc ggagactcgg ccatcaagcg catcatctgc
     7321 ggccagtgat gccagagaaa aaaccccgtc acgctgccaa ccaggatggt tttcaaaatt
     7381 gctttaagca actccgcgcc agtctgagcc gagaacatcc gtttaatgcc cggtagcggg
     7441 ttgagtttgg aaaacttcgg ctgcaaggat ttgccgctaa ataccagccc tcccagcatg
     7501 accggagaaa tgagcgccac cagcaccacg ccgctaatca gcggcagcag cgccagcatg
     7561 gcttctctga tcagcagaat aatctgcccg aggatcagat tcgggtcatt gataatactg
     7621 tgatcaaaat gcagcccagc ggagagcatg cccgacaatc gacgggccag cgacacaccg
     7681 ccaaaccaga taacactaac gcccaccagc aaaatcagca gtgaggtcag ttcacgggaa
     7741 cgcgggattt gcccctcttc ccgcgctttt tctagtcggt gaggtgtggg ggcttctgtt
     7801 ttgtcgtcgc tctcgtcaga cacgtcgcca atcctggaaa gagtattaag cgtgaatgat
     7861 gccagagcgc aaagcgttca atggtttgag taaggggcaa aacaggcggg atttagggct
     7921 tttgctgcca cacatcaagc atagtgtgcg tttgtcggat gcggcgtcat cgccttatca
     7981 gaccgcctga tatgacgtgg tcacgccaca tcaggcaata caaatcaaaa tccaagacta
     8041 tccaacaaat cgtccacctg atcctgactg gctaccacac cggctttgct ggtatcgacc
     8101 tgaggtccat taagcaaact ctggttttca cgttttggac gcgactcctg ttccgggatg
     8161 ttttccaaca gcaccatcag caactggcgt tcgatctcct gaatgacatc catcatccgc
     8221 ttaatgacct gcccggtgag atcctgaaaa tcctgcgcca tcatgatttc cagcagttgc
     8281 gcgttagtaa agctggtatg cgcgggtaca tctgccagaa attgtcgtgt atctgttacc
     8341 agttcacggg cgtcggcaag gtcaatcgga tcggcaaacc agtcatccca acgttgggtt
     8401 aacgcttttg ctgatttctc catttgatcc tgatgcggtt gtgacgcctc aacactgttc
     8461 agcgcccgct ccgcagcctg ggcggtcatc tgcacaacat agtacaaacg atcgcgcgca
     8521 tcggggatgg cttccgccgc ttcggcaatg gcctgatcca gccccagttc ccgcaaactg
     8581 tcgcgcagca tacgcgtcag gctgccgatg cgcgcaatga tatcgccagc tgaatgctcg
     8641 tcagcaggtt tgattgatgg ttgcatcata gtcgcatcct cacatgccca gtttctcaaa
     8701 gattttgttg agtttttcct ccagcgtcgc ggcggtaaat ggcttcacca catagccact
     8761 ggcccccgct tgcgccgcag caatgatgtt ctctttcttc gcttctgcag tcaccattaa
     8821 cactggcaat gccgacatcg cgccatccgc acgaattgtt ttcagcaatt ccaggccatc
     8881 catattgggc atgttccagt cggagataac aaatccataa ccgcctgcct gcaacttatt
     8941 gagagcgtcg acgccatctt ccgcttcctc aacattattg aatcccagct ctttcagcag
     9001 gttacgcact atgcgtcgca tggtggaaaa gtcatccaca accaaaaatt taagttcttt
     9061 atccgccatt tcacactcct gatttaaata cgtatcgcct gtccggcact aatttttgcc
     9121 aacatttgct ggcttacctg gctaagatcg accacttcgc agacaccacc catattgatg
     9181 gcctcgcgcg gcatgccgaa caccacgcaa cttgcttcgt tttgcgcaag ggtccatgcc
     9241 cccgcctgac gcatcgccaa cattcccgcc gcgccgtcgt tgcccatacc ggtcaggatc
     9301 accccaaccg cattacgccc cgcctgtttg gcgacagaat ggaacaacac atctaccgaa
     9361 ggccgatgac ggttaaccgc cgggccatcg tgaattttga tttggtaatt tgcgccacta
     9421 cgcgacagct ccatatgccg atcgcccggc gcaatatagg catgccccgg caagacacgt
     9481 tctccgtctt cggcttcttt aaccccgatc tggcaaagct tattaagtct gtcggcaaaa
     9541 gagcgggtga aaccgggcgg catatgttgg gtaattaaca atgccgggct ggaaagcggc
     9601 aacggttgca gtacgtgacg aattgcctca gttccacccg ttgaagcacc aatcgcaatc
     9661 agtttttcag aactcaacaa cggacccgcc ttcagcgttg tcggtgctga caatggctta
     9721 tgtgctgcaa ggctcgcctt tgctgccgta cgcacctttt cagcaatcat ttcgctatac
     9781 gccagcattc cttcgcgaat acccagttgc ggtttggtga caaaatctat tgcccccagc
     9841 tccagcgcgc gcagcgtgac ttctgatcct ttgccggtca aggaagaaac catcacaacg
     9901 ggcattggac gcaaacgcat taatttttcg aggaaatcca gtccgtccat ccgcggcatt
     9961 tcaacatcca gcgtcagcac atcgggattg aatttcttaa tcaagtcacg cgcgaccagc
    10021 ggatcaggcg cggtcgccac catttccatg tcgctatggc tgttgatgat ttctgtcatg
    10081 atctggcgca tcagtgccga atcatcgaca gataacaccc tgattttgct catcgttaat
    10141 ccttacttag cgcatacacc gtctgaccac gcagcgtgaa gcggcgctca aggtggctaa
    10201 agttttcaga gtgacccgca aacagcaatc cgtcgggttt aaggagcgga acaaagcagc
    10261 gcaaaatctc ctgctgggta ttttgatcga agtagatcat gacgttacga cagaagatcg
    10321 catcaaacgg ccccggcacg gtgtactgtt tcgccagtag attcagcggg gcaaaatcaa
    10381 catagttcgc cagctcctga cgcacgcgta ccagcccttc atgcggtccc gtccctcgca
    10441 tgaaataccg ttgcagttgc tgcggcgtca ggtttttcaa ctcttcatgg cgataaatac
    10501 cgcttctggc tttttccagc acttcggtgt cgatatcact ggcaaacact ttccagcgtc
    10561 cgggcgcggt gcccaatgtg tcagccagcg tcatcgcaat gctgtacggc tcttcgccgg
    10621 tcgaagccgc cgcgctccat acgcgatact cgccagaacg gcgacgtgcg tgatccgcga
    10681 gcagagggaa atggtgtgcc tcacggaaaa atgccgtcag attcgtggtc agcgaattga
    10741 taaacgcctg ccactcaccg ctgtgctgat tagattccag caagttcaga taatgaccga
    10801 aatccgtcag tcccagcgaa cgcaaacgac gaaccagtcg gttgtaaacc atgtcgcgtt
    10861 tatggtcagc cagaacgatc ccggctcgtt gatagatcaa ttgacttatc cgccgaaaat
    10921 gcgcgtcgga aagcgccagg cgctcggtca tctgtaacaa taaagacgtt tgcccacagg
    10981 gcagagatga agtcatagcg ccttctcaat cacttcagga taccactggc gcaatttgta
    11041 actgcgccga ctcatgtcgt gctacttcat gttcttcaag ggtaaatacc gccacacgcg
    11101 acgaaagatg gtcggcctga ttcgccagtt gttccgttgc caccgccgct tcttctacca
    11161 gcgaggcgtt ctgctgagtc acctgatcca tctggctgac agcctgtgca acctgctcaa
    11221 tcccccgctg ttgttcttcc gacgcagagg caatttctcc cataatgtcg tccacgcggg
    11281 tcaccgaact gacaatatcg atcatggtcg cggcggcgtt attcaccagt ttcgaaccct
    11341 gctggacacg attgactgac tcttcgatca gccctttgat ctcttttgct gcctgcgcgc
    11401 tacggctggc aagattgcgc acttcacctg ccactaccgc aaaaccacgc ccctgctctc
    11461 cggcgcgagc cgcttccact gccgcattca gggccagaat attggtctgg aaagcaattc
    11521 cgtcgataac gctgataatg tcgccaattt tttgcgagct ggtggcgatc tcctgcatgg
    11581 tgtgagtcat ggtactgacc tgaacaccgc ccgcctgcgc cgttgtcgcg gcattttttg
    11641 ccagttccga cgcctgtcgt gcgttatcgg cgttttgccc taccgtggcg gttaattgct
    11701 ccatactggc ggccgtttgt gccagcgatg ccgcctgctg ttcggtacga cttgagagat
    11761 cgttattgcc tgcgacaatc tccgcgatac caatgtgcat ctcctggctt cccttacgca
    11821 catcactcac cgtcccacgc aaagcctgct gcatggtctt caggctggca aaaatggcgg
    11881 tgatctcatt acgaccatat accgcaatcg gacgcgccag attacccgca gcaatgctgt
    11941 caaaatggct accgataatg gccagtggtt gaacaatcat cttgcgcgtc caccacagcg
    12001 cactgctgat gtagatcgcc gcaacaataa tcatgctgat aaacaccagc gccgaaatct
    12061 gatagttacg ctggctttgc gcactggcgg cttccagcac atggttgatc tccagctgcc
    12121 aggcctcaaa gttaacgtca aacgcattct gtgattcctg caccggcgca gtgaggaaat
    12181 ccgaaagttg attactttca agccaggtcg cctgatgttc gagatcgttg ttgtgccagc
    12241 gggcaaaact tttctccgtc tctttttgca acgccctgac gtgctcgttg cccgcagtca
    12301 tcgccataaa acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca
    12361 tcaacgtttt aatgtcatcc gccggatagc taagtgccgt cagagtgccc gctttgttca
    12421 gcgcggtact ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat
    12481 tgctctgctc gacctgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc
    12541 cgttactgcc aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa
    12601 ttcgaatacg attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaata
    12661 ctcgtcaaaa tgtttcccag tttggagctt gttcagcaat tcgcagtcgt ggctgtgccg
    12721 gtggttgctc actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg
    12781 ccagacggaa cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagcgg
    12841 cggcggcagc tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg
    12901 aaaccgccaa tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct
    12961 cgcccataat gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg
    13021 cgctttcgac cagcaccgaa ccggtatcaa cgcgtgagac ggagtcttca atgagggctt
    13081 tgatctcttt tgccgcctgg gcgctgcgac tggcaagatt acgcacttca cccgccacca
    13141 cggcaaaacc acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga
    13201 ggatattagt ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg
    13261 aactatcggc gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct
    13321 gggcggtgtc ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt
    13381 gcttcactgt cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct
    13441 gctgttcagt acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat
    13501 agatggcatc tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat
    13561 gtgaaacgct ctgcgccagg tcgcccattt cactgcgccc gtcaatggtc agggtattcg
    13621 ccaggttacc accggcgatt tcgcgaatgt gagcaataat ttttgccagc ggagtaagca
    13681 acatacggcg aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa
    13741 ccgccagttg ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat
    13801 acagtttttc actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc
    13861 cctgggttgg ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt
    13921 cagttaacgc tgtgtgatag tttttatatt tttcatcaat attacgactg gtagcgacca
    13981 tttcaggtaa cggtgccatg cttttgaatt ttttataatg agtcgcggcc tgggccaatg
    14041 ttttcctggc gctatcgagc aattcaactt tggcgttact ttgttgattg gaggaatcca
    14101 tcatcatccg caccgctgaa cgactcaggt taatgcgcgt ttgcagcatt aaatcccagg
    14161 ttgacgtcag ctcgccctgc tgttcccgta attgattgga aaccacaaag ctcttctggc
    14221 tatggtgaag ggaagaaaaa aacagactgc cggaaataag ctgtaacagt gcgaataccc
    14281 ccagcaccat taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg
    14341 aaaacaagtt aatctcgtta tcggcaagaa ggggggaaac tttattgctg atgccacccg
    14401 ccgcgaaatt gaaataaaaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa
    14461 atcgggagaa ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt
    14521 tcagcagttt ttcgatgttc accagaatca acatccggtc gcccagtgcg cccagtccag
    14581 tgagatattc tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg
    14641 tcaatgaaag cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat
    14701 tcaggacgat aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc
    14761 gtaagtcaac aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg
    14821 gcgtgttcgc aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat
    14881 caataccgta ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc
    14941 tggccagctt tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc
    15001 ggcggtgttc gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat
    15061 cagtgccacg ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata
    15121 gttactttca aggtttttaa ccacaacctg gtgctgacca attaattgat ccaccagcaa
    15181 ggcgtagcgg cgaccgccac tttgtaagat caccacaatt ccctgggtgg cttcggtttt
    15241 cgcgcccgcg acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac
    15301 ttccagcacc cgctcgccgc cggccagtgg atgcagatcg gcttcacggg gttgcagtga
    15361 ttccataaca gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta cggacatgcc
    15421 gtcgaggatg gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt
    15481 cgactggatt tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc
    15541 gacgccgcgc ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat
    15601 cagcatcgcg acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc
    15661 ttttgccaga attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat
    15721 gttgccgccc tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc
    15781 gagccgtttt tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag
    15841 cgggtcgata atgcgttcta tcaggctttt gtcgagttca gtagaactgc ccaccagcgt
    15901 cagttctacc tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact
    15961 aaaaacatat tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc
    16021 gttacgttgt aactgcccca tgctggttat caaatcacca tgattaaccg ggtccagttc
    16081 gctggaacgc tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa
    16141 ttgatcaacc ttttctaccg ctacacggat gctggtggat tcattgctgc gcgtcgtttt
    16201 ttcccgctcc acgcggccgg ttggcgcttg ttcggctgcc agtttaagca ctggtggggt
    16261 ggatattttt ggcgagactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa
    16321 acagagtacc gctgtgatgt catcttcggc gatgtcgccc ggtaatattg ccgagagcga
    16381 atccgcccct ttcaccacgt cagttaacgt tgtcagatgt cccagttctt cttccagcag
    16441 gtcgacttcc ccggccttca ggcgcgaaag gataattcgt cgcggcgact gactgcgact
    16501 ctgctcatct tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt
    16561 ttcgcctttc gcttctaatg ccagttgacg caaggcctgg cagatataat cgaagctggc
    16621 ggcatccggc tcttgcgact gtttataagc gtcgagctgt tcttgcatga tgtccttcgt
    16681 ttccaaaaac agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc
    16741 gagcaggttt tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aagttcctgc
    16801 ccctcctttg atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc
    16861 ttccggctgc aaaaccagca gatgctgctc catgtcagcc aacagttcgt ccgcttcatc
    16921 aaaaaatgtc tgataaaaat cgcttatatc catgctcacg ctgtcacctc ggttcggctg
    16981 atggcattgt gggaacactg acctgtggtg caacctcagg tttttccagg gcgcttactg
    17041 gctcattctg gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagta
    17101 ccagcaggct gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg
    17161 ttgccgccat gccgacgaca cgtaacactt tgccgctatc caaccctccg accatcagtt
    17221 cgcggcggga tgcattggcc cgatcggcag aaagctccca gttgctatat cctttctcac
    17281 cgctggcgta ggggaaatca tcggtatgac ctgaaaggct aatacggttg ggaataccgt
    17341 tcagtacagg cgcaatggca cgcagaatgt cgcgcatata gggttcgaca tcggcactgc
    17401 cggttctaaa catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga
    17461 ccagatcgat tttgagatgg ggacgtaacg cccgcagttt cggatcggac tctatcaact
    17521 ggtcgagatc accccgcaat ttccgcaatc gactttgctc catgcgtttt ttcagctctt
    17581 cgatgttcgg ctgcttattc acttccccct ggctttgggt gtaatcatca ccaccgccgg
    17641 gaattgggct ttcactatta gaaatgcgat cgccgcccgt aaccgcagtc gccagtggag
    17701 tccggaagta ctccgcaatc tgaatcagct cttttgggct ggagatggag atcagccaca
    17761 tcaccagaaa aaaggccatc atcgcagtca taaagtcggc ataagcaatc ttccacgatc
    17821 catgtgctgc cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt
    17881 gattcttcat gcttcctcgg ttgtcatctg ttgttgcgga tttttcaccg cacgcacatg
    17941 ctcttccagt tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc
    18001 aacggcgata ggcggtgcgt aaccgttcag attagaaagc agagtgactt tgacgcactg
    18061 catcattttg ctggtttcgg cgcttttctg acgtaaaaca ctcgctaatg gggaaataaa
    18121 tccgtaagcc aataaaatgc cgaggaaagt ccccaccatc gcatgtgcga taagcgcacc
    18181 cagctcggcg gcaggacgat cggctgaacc taacgcgtga acgaccccca ttacagccgc
    18241 gacaatacca aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc
    18301 tgcctcgctt tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt
    18361 catatgaccg ctgataatca gacgcagata atcgacgata aaatcaagca tgacgctatc
    18421 cgcgaggatg cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg
    18481 ttccagcgaa aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag
    18541 cagatccata tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcctt
    18601 cagcgtgcct ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc
    18661 ggcaataatc accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa
    18721 ataaccgccg aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc
    18781 atccttccac tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg
    18841 gcaaaaaaaa gcagcggtac gtcgttaccg ctgctggaat gttgcgcctc accgtatcag
    18901 ttaaacagcc tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg
    18961 ggaaagttta cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct
    19021 gccaacaggc tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag
    19081 ttgcagtaat ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag
    19141 tggtccttct tctgcttgtg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc
    19201 atcgacgcca ttacacaaac cggttttcag taaaaactgc catgcattac agaacatcga
    19261 agcatgaacg ttttgttccc aggtcataaa ccagtcggtt gagaatggca gcatgccttt
    19321 cggcggtggg cttccgcgca gttctttata aagttttatc aggcgtccgc gacttaactg
    19381 tgtttcgctt tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg
    19441 aatatcccgc gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc
    19501 agcgcttctt caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta
    19561 tgaatttgct ggagatcgtc aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg
    19621 ctgtcaaaac ggaagtgaca aaccagttga ttggtttctg ccagcttaac catttgcgga
    19681 agagtcagtg ccgctaacgt tgtcgccatt tcttcattta tgccgagacg aaacatagcg
    19741 gacgctttgt cctgaacaat caaacgctgt gcaagtagta aatatgacaa gttgatgtca
    19801 taaatgtgtt tcagcaactc ggaggtatgc attattccca cccagaataa ccaactttat
    19861 ttttatgcgg tttcaccgca ccccgtgatg tcgccgggaa gccccggtaa aaaataatta
    19921 gcattagaat agttgcgata agctgcaata agcagaacca cctttttggt ttaatatgtc
    19981 cttacaaata gaaatgggtc tttacactta tctaagattt ttcctaaatc gacgcaactg
    20041 tactcgtcac tacacgcaca tacaacggag gggggctgcg attttcaata atgtgtgatg
    20101 cagatcacac aaaacactca attacttaac ataaatgtat aagtcatact tttgttttgg
    20161 gtgtatttca atctgttaaa aagtttttcg ctacgctagc aagcaaaaat gaaacaggaa
    20221 taatcgaaat gggatgttgc gcacagttaa aataactcac cgtaaataat catctgctat
    20281 aaataatcac tttcatgcaa taccagataa gctattttta aacagacact taccgcacaa
    20341 caaactaatt aaatacaaag aaaggcaaca atcaacttaa
//
LOCUS       DAZFWL020000082        20266 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFWL020000082 DAZFWL020000000
VERSION     DAZFWL020000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.082"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(404..1600)
                     /gene="hemY"
                     /locus_tag="ACHKNP_RS15645"
                     /old_locus_tag="pgaptmp_003129"
     CDS             complement(404..1600)
                     /gene="hemY"
                     /locus_tag="ACHKNP_RS15645"
                     /old_locus_tag="pgaptmp_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HGU4705255.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1603..2820)
                     /gene="hemX"
                     /locus_tag="ACHKNP_RS15650"
                     /old_locus_tag="pgaptmp_003130"
     CDS             complement(1603..2820)
                     /gene="hemX"
                     /locus_tag="ACHKNP_RS15650"
                     /old_locus_tag="pgaptmp_003130"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HGU4705256.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            complement(2842..3582)
                     /gene="hemD"
                     /locus_tag="ACHKNP_RS15655"
                     /old_locus_tag="pgaptmp_003131"
     CDS             complement(2842..3582)
                     /gene="hemD"
                     /locus_tag="ACHKNP_RS15655"
                     /old_locus_tag="pgaptmp_003131"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HGU4705257.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3579..4520)
                     /gene="hemC"
                     /locus_tag="ACHKNP_RS15660"
                     /old_locus_tag="pgaptmp_003132"
     CDS             complement(3579..4520)
                     /gene="hemC"
                     /locus_tag="ACHKNP_RS15660"
                     /old_locus_tag="pgaptmp_003132"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HGU4705258.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4907..7453
                     /gene="cyaA"
                     /locus_tag="ACHKNP_RS15665"
                     /old_locus_tag="pgaptmp_003133"
     CDS             4907..7453
                     /gene="cyaA"
                     /locus_tag="ACHKNP_RS15665"
                     /old_locus_tag="pgaptmp_003133"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HGU4705259.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDNDTPLLQQYFS"
     gene            complement(7493..7813)
                     /gene="cyaY"
                     /locus_tag="ACHKNP_RS15670"
                     /old_locus_tag="pgaptmp_003134"
     CDS             complement(7493..7813)
                     /gene="cyaY"
                     /locus_tag="ACHKNP_RS15670"
                     /old_locus_tag="pgaptmp_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HGU4705260.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7861..9186)
                     /locus_tag="ACHKNP_RS15675"
                     /old_locus_tag="pgaptmp_003135"
     CDS             complement(7861..9186)
                     /locus_tag="ACHKNP_RS15675"
                     /old_locus_tag="pgaptmp_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HGU4705261.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            complement(9183..10691)
                     /locus_tag="ACHKNP_RS15680"
                     /old_locus_tag="pgaptmp_003136"
     CDS             complement(9183..10691)
                     /locus_tag="ACHKNP_RS15680"
                     /old_locus_tag="pgaptmp_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HGU4705262.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            complement(11029..11583)
                     /locus_tag="ACHKNP_RS15685"
                     /old_locus_tag="pgaptmp_003137"
     CDS             complement(11029..11583)
                     /locus_tag="ACHKNP_RS15685"
                     /old_locus_tag="pgaptmp_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HGU4705263.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11933..12136
                     /gene="yifL"
                     /locus_tag="ACHKNP_RS15690"
                     /old_locus_tag="pgaptmp_003138"
     CDS             11933..12136
                     /gene="yifL"
                     /locus_tag="ACHKNP_RS15690"
                     /old_locus_tag="pgaptmp_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4705264.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            12173..12997
                     /gene="dapF"
                     /locus_tag="ACHKNP_RS15695"
                     /old_locus_tag="pgaptmp_003139"
     CDS             12173..12997
                     /gene="dapF"
                     /locus_tag="ACHKNP_RS15695"
                     /old_locus_tag="pgaptmp_003139"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HGU4705265.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12994..13701
                     /gene="yigA"
                     /locus_tag="ACHKNP_RS15700"
                     /old_locus_tag="pgaptmp_003140"
     CDS             12994..13701
                     /gene="yigA"
                     /locus_tag="ACHKNP_RS15700"
                     /old_locus_tag="pgaptmp_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HGU4705266.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            13698..14594
                     /gene="xerC"
                     /locus_tag="ACHKNP_RS15705"
                     /old_locus_tag="pgaptmp_003141"
     CDS             13698..14594
                     /gene="xerC"
                     /locus_tag="ACHKNP_RS15705"
                     /old_locus_tag="pgaptmp_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HGU4705267.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            14594..15310
                     /gene="yigB"
                     /locus_tag="ACHKNP_RS15710"
                     /old_locus_tag="pgaptmp_003142"
     CDS             14594..15310
                     /gene="yigB"
                     /locus_tag="ACHKNP_RS15710"
                     /old_locus_tag="pgaptmp_003142"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HGU4705268.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREVEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            15394..17556
                     /gene="uvrD"
                     /locus_tag="ACHKNP_RS15715"
                     /old_locus_tag="pgaptmp_003143"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             15394..17556
                     /gene="uvrD"
                     /locus_tag="ACHKNP_RS15715"
                     /old_locus_tag="pgaptmp_003143"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HGU4705269.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(17703..18467)
                     /gene="yigE"
                     /locus_tag="ACHKNP_RS15720"
                     /old_locus_tag="pgaptmp_003144"
     CDS             complement(17703..18467)
                     /gene="yigE"
                     /locus_tag="ACHKNP_RS15720"
                     /old_locus_tag="pgaptmp_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HGU4705270.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            18629..18685
                     /gene="ysgD"
                     /locus_tag="ACHKNP_RS15725"
                     /old_locus_tag="pgaptmp_003145"
     CDS             18629..18685
                     /gene="ysgD"
                     /locus_tag="ACHKNP_RS15725"
                     /old_locus_tag="pgaptmp_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HGU4705271.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18837..19787
                     /gene="corA"
                     /locus_tag="ACHKNP_RS15730"
                     /old_locus_tag="pgaptmp_003146"
     CDS             18837..19787
                     /gene="corA"
                     /locus_tag="ACHKNP_RS15730"
                     /old_locus_tag="pgaptmp_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HGU4705272.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19829..20266)
                     /locus_tag="ACHKNP_RS15735"
                     /old_locus_tag="pgaptmp_003147"
                     /pseudo
     CDS             complement(19829..20266)
                     /locus_tag="ACHKNP_RS15735"
                     /old_locus_tag="pgaptmp_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF943 family protein"
BASE COUNT         4739 a         5230 c         5259 g         5038 t
ORIGIN      
        1 atcattcata ataagtatgt gtaggatcaa gctcaggtta aatattcact caggaagtta
       61 ttactcagga agcaaagagg attacagaat tatctcataa caagtgttaa gggatgttat
      121 ttcccgattc tctgtggcat aataaacggg tagatgctca ttccatctct tatgttcgcc
      181 ttagtgcctc ataaactccg gaatgacgca gagccgttta cggtgcttat cgtccactga
      241 cagatgtcgc ttatgcctca tcagacacca tggacacaac gttgagtgaa gcacccactt
      301 gttgtcatac agacctgttt taacgcctgc tccgtaataa gagcaggcgt ttttttatgc
      361 atcagaaagg tcccggaggt gcttgcctcc gggtgagaag gaactactgt ggtgggttgt
      421 tctgcaacgt taacatcaaa ccgtcgcgac gcatcgctgc agcttcttcc ggcttgtgca
      481 gtctgtccag cgcgtcggca agccatgcgt aatcgtaggc gtccggacgt tgtttcagcg
      541 ccgcgcggaa ggcgagcgat gcttcctgcc attctccgtg cttcatcagt gactggccca
      601 gtgtgctcca caacagcggg cgatcgccga cgtttttgat ctgctggcgc agcacttttt
      661 ccagctgttc cggattgttg gttttcagtc gaggaatcgg cagcagtagg cgatcgtcgt
      721 actggcgttt caggccatcg atgataattt gctgggcagt atcatgatcg tcacattcaa
      781 taagatgttc cgccattgcc acctgcaacg ctacctgatg acgcgttttc cggctttggt
      841 ttttccacca gttacgcaaa ccttcactac cgttatcggc acgcgcctga tccatcaggc
      901 caatccatgc ctgttgttcc agcattgcac gatgttcttc atcaccaaca tgggctttcg
      961 ccattgatgg gatgatatcc agcagcgaac tccatgcacc tgtgcggata tacgcctgtt
     1021 ccgccagacg taatacttcc ggatggcgtg gcgtaacttc cagcagctta tccacgccgt
     1081 ggcgtgcagc atggttttca ttacgggcca gttgcagacg tacacgggtg atttctaccg
     1141 gaatggtgtc attgccggcc agctctgctg cgcgttccag atgttggttg gcgcgtgctt
     1201 catcaccacg ttgttgcgcg gcttcggcag ccagtagata gttcaccacc ggttgttccg
     1261 cgtgatcggc atttttcgcc atcagctttt caacttgctg ataatcgcct tccgccagtt
     1321 tcagcagcgc ctgttcggtc tgcttacggg cacggcgacg cttacgtccg acaaaccacc
     1381 cacgggtgtg tgcgcctgtg cggaagatcc gccgcagtag ccactcaatg gcaaacagca
     1441 ctaccatcgc cagaatcaaa ataatcgcca ggcccgtgac gctggtttcg atattgtagt
     1501 tgtcagtctg gatcagcacg taaccctgat gaccggcaat catcgggcca accactatcc
     1561 ccgcaatcag caaaacaaag agtaataaca cttttagcat ggttattctc cttgcggcgc
     1621 ggctgccgga gtatcacctt gcggtgcagg tgcaggtgca ggtgcaggtg caggtgcagg
     1681 tgcaggtgca ggtttagctt ccgttgtccc cgctgccggt tgtgccagca ggttacgcac
     1741 gcgggtctgc atcagttttt ccagcatcgc ctggctttgc agggtttccg gaagatccat
     1801 cgagatattt tgctggctta actggtccac ctcgtcgagg aacgctttgg tggtggcatc
     1861 atcagtatcg tagtaagctc gtacccaggt ggagacgttc tccagcgcct ggcgataagt
     1921 ctcttcctgg tgacgcggta cagcttgtgc tgcgaccagc aggcgagagc gaatattttc
     1981 gcgcagatag atatcctgat ttggcgctaa cagcggtacg gcggtgtcat cacgacggcg
     2041 aatcgtaatg aagttgtcca taaagttctg ccagcttttt tgcagattga tacgccattc
     2101 gctgatggaa ctggaaagct cttcaccgtc ggaatccatc ggcgaaccat cgctgtcatt
     2161 atcggccaga cgcaggttat ctacctgatt tgaaagctga ttaagcttaa ggatgatgcc
     2221 gtcataatcc acctgcgaaa ctgcagaaag gctggcgata tcatcggtaa ttgcccgacg
     2281 aacggtaatc agactcgggt cattcatatc cgccaggctg gcatctgcac ttttcagcaa
     2341 cgctgcagcg gtcgtgacgt cctgatcgct ccacagcttc cgtccggcga gtttcaccag
     2401 aaaatcggcc tgagccagca gccaggtttt agcatcgctg ccggaaatgg tggcgacctt
     2461 ttgttggact tcatccaact gttttgccag cgtttcttgc tgacgattcg cctgcttaag
     2521 ctgtgcagct tgttgcttaa taatgccttc cagctcggct ttttggctct cctgggcttt
     2581 ttgcaatgcc gtcagttggt tagccagggc atcgctggtg gcggtctgat tgacggcctg
     2641 ttgtttaccc cagccataca aaccgacacc cgccgccaga gcaatagcga tagccaccgc
     2701 gctgagaatc aatgcagtat tgttcttact ctttttttct gttgcgacag gttgtgacgt
     2761 ggtgtccacg gcctccctgg tctcttcaac cacggcggag gttttttctt gttccgtcat
     2821 tatggcttcc tgttatgaga gttattgtaa tgcccgtaaa agcgcatcgt tgtcagcgtt
     2881 atcggcgacc ttaatgtctt gccagcccag ttcccgggcg agtttcgcca aacgctcact
     2941 gacgaccaat agtcgacagt gtagtaacca gtgctcacga taccattgtg ggatcagcga
     3001 ccagagttgc tgcaacattt caccgctggt aacaacgacc atcgtcacct cgcgggattg
     3061 ccagcgcatc gcttcttctg caccatcgta atggattgcg catcgttgat aacattcaca
     3121 aaaagtgacc tcagcaccgc gcgctgtcag ggtatcccca attagctcac gaccgccatt
     3181 gccacgtaat atcagcgcac gtttgcccgc aatattttgt aattcaggta attgtagcaa
     3241 gacttcgctg atttcccgat cctgcgggta gagaatcttc tgtccgctta cggtatgtag
     3301 cgccagtgcg gtggtgcgtc caatggcgaa ataatcaggt agtcggggcc atttacgatc
     3361 ttgctgatgc agctgtgatt gggcaaaagc aaccgcgtgt tgcgagaggg caaacaacag
     3421 atcgctctcc cccagcgcag ccagttgatc agcaagttgc ggtaattgtc gacccggaga
     3481 aaactcaatc agcggaaaat gccaggccac ctgccccagt gtgcgcagac ggctcactaa
     3541 ctcttctcca gcgggagacg ggcgggtgac caggatactc atgccggggc gtctccgtta
     3601 tagacttcag cgaggatctc gcgcgcgccg ttattcagta gctcttctgc cagcgaaatc
     3661 cccatttgtt cggcattttg cggcgcaccg cggcgttcac cgcgaataat ctgcgaaccg
     3721 tccggcgcgc cgaccagcgc acgcagccag atttcaccat caataagctc ggcgtagcta
     3781 ccaattggca cctgacatcc accttcgaga cgggtattca tggcgcgttc tgcggtaacg
     3841 cgcagtgcag tttcgtggtg attcagcgcg gcaagcagct cgcgagtgcg cgtatcatca
     3901 aggcggcatt caataccgac tgcaccttgt cctaccgccg gaagagaaat ctcgggtggt
     3961 aacgcggcgc gaatgcggga ctccagacct aaacgtttta gtccggctac ggcaagaatg
     4021 atggcatcgt attcgccgtt atctagtttg ctcaggcgag tgccgacgtt gccgcgcagg
     4081 gagcggataa tcagatccgg gcggcgttca gccagttggc actggcgacg taaactggac
     4141 gtcccgacga tactgcctgc cggtaacgca tccagactgt cataattatt ggacacaaag
     4201 gcatcgcgag gatcttcacg ctcacaaata gtgaccaacc ccagaccttg cgggaattca
     4261 accggcacat ctttcattga atgtacggcg atatcggcgc gattctccag gagcgcgact
     4321 tccagctctt ttacaaataa gccttttccg cctactttcg ccagcggcgt atcaagaatc
     4381 acatcgccgc gcgtcaccat cggtaccagt tcaaccacca ggcccggatg gctcgccatc
     4441 aacttgtctt tgacatagtg tgcctgccag agtgcaagtg ggctttggcg tgtggcaatt
     4501 cttaaaacat tgtctaacat gcttgttacc gtcattatca tccgtggtcc atcctaacat
     4561 ccttgccaga gtgatgtcag tgttgtggtg aaacgtagac gcctgcgcaa accgtaaaat
     4621 gaggtctggc agtggatcct gacaggcgtt tcacgccgtc gtaataagga atttacagag
     4681 aataaacggt gctacacttg tatgtagcgc atctttcttt acggtcaatc agcaaggtgt
     4741 taaattgatc acgttttaga ccattttttc gtcgtgaaac taaaaaaacc aggcgcgaaa
     4801 agtggtaacg gttacctttg acatacgaaa tatcccgaat gccgcgtgtt accgttgatg
     4861 ttggcggaat cacagtcatg acgggtagca aatcaggcga tacgtcttgt acctctatat
     4921 tgagactctg aaacagagac tggatgccat aaatcaattg cgtgtggatc gcgcgcttgc
     4981 tgctatgggg cctgcattcc aacaggtcta cagtctactg ccgacattgt tgcactatca
     5041 ccatccgcta atgccgggtt accttgatgg taacgttccc aaaggcattt gcctttacac
     5101 gcctgatgaa actcaacgcc actacctgaa cgagcttgaa ctgtatcgtg gaatgtcagt
     5161 acaggaccca ccgaaaggtg agcttccaat tactggtgta tacaccatgg gcagcacctc
     5221 gtccgtaggg caaagttgtt cctctgacct ggatatctgg gtctgtcatc aatcctggct
     5281 cgatagcgaa gagcgccagt tactacaacg taaatgtagt ctgctggaaa gctgggccgc
     5341 ctcgctgggt gtggaagtca gcttcttcct gattgatgaa aaccgcttcc gtcataatga
     5401 aagcggcagc ctggggggcg aagattgtgg ctccacccag catatactgc tgcttgacga
     5461 attttatcgt accgccgtgc gtctcgccgg taagcgtatt ctgtggaata tggtgccgtg
     5521 cgacgaagaa gagcattacg acgactatgt gatgacgctt tacgcgcagg gcgtgctgac
     5581 gccaaatgaa tggctggatc tcggtggctt aagctcgctt tctgctgaag agtactttgg
     5641 tgccagcctt tggcagctct acaagagtat cgattcccca tacaaagcgg tactgaaaac
     5701 actgctgctg gaagcctatt cctgggaata cccgaaccca cgtctgctgg cgaaagatat
     5761 caaacagcgt ttgcacgacg gcgagattgt atcgtttggt ctcgatccat actgcatgat
     5821 gctggagcgt gttactgaat acctgacggc gattgaagat tttacccgtc tggatttagt
     5881 acgtcgctgc ttctatttaa aagtgtgcga aaagctcagc cgtgaacgcg cctgcgtagg
     5941 ctggcgtcgc gcagtgttga gccagttagt gagcgagtgg ggttgggacg aagctcgtct
     6001 ggcaatgctc gataaccgcg ctaactggaa gattgatcag gtgcgtgagg cgcacaacga
     6061 gttgctcgac gcgatgatgc agagctaccg taatctgatc cgctttgcgc gtcgcaataa
     6121 ccttagcgtc tccgccagtc cgcaggatat cggcgtgctg acgcgtaagc tgtatgccgc
     6181 gtttgaagca ttaccaggta aagtgacgct ggtaaacccg cagatttcac ccgatctctc
     6241 ggaaccgaat ctgaccttta tttatgtgcc gccgggccgt gctaaccgtt caggttggta
     6301 tctgtataac cgcgcgccaa atattgagtc gatcatcagc catcagccgc tggaatataa
     6361 ccgttacctg aacaaactgg tggcgtgggc atggtttaac ggcctgctga cctcgcgcac
     6421 ccgtttgtat attaaaggta acggcattgt cgatttgcct aagttgcagg agatggtcgc
     6481 cgacgtgtcg caccatttcc cgctgcgctt acctgcaccg acaccgaagg cgctctacag
     6541 cccgtgtgag atccgccatc tggcgattat cgttaacctg gaatatgacc cgacagcggc
     6601 gttccgcaat caggtggtgc attttgattt ccgtaagctg gatgtcttca gctttggcga
     6661 gaatcaaaat tgcctggtag gtagcgttga cctgctgtac cgcaactcgt ggaacgaagt
     6721 gcgtacgctg cacttcaacg gcgagcaatc gatgatcgaa gccctgaaaa ctattctcgg
     6781 caaaatgcat caggacgccg caccgccaga tagcgtggaa gtcttctgtt atagccagca
     6841 tctgcgcggg ttaatccgta ctcgcgtgca gcaactggtt tctgagtgta ttgaactgcg
     6901 cctttccagc acccgccagg aaaccgggcg tttcaaggcg ctgcgcgttt ctggtcaaac
     6961 ctgggggttg ttcttcgaac gcctgaatgt atcggtacaa aaactggaaa acgccatcga
     7021 gttttatggc gctatttcgc ataacaaact gcacggcttg tcagtgcagg ttgaaaccaa
     7081 tcacgtcaaa ttaccggcgg tggtggacgg ctttgccagc gaagggatca tccagttctt
     7141 cttcgaagaa acgcaagacg agaatggctt taatatctac attctcgacg aaagcaaccg
     7201 ggttgaggtg tatcaccact gcgaaggcag caaagaggag ctggtacgtg acgtcagtcg
     7261 cttctactcg tcatcgcacg atcgcttcac ctacggctca agctttatca acttcaacct
     7321 gccgcagttc tatcagattg tgaaggttga tggtcgtgaa caggtgattc cgttccgcac
     7381 aaaatctatc ggtaacatgc cgcccgccaa tcaggacaac gatacgccgc tattacagca
     7441 gtatttttcg tgatgagcgt gccggaaagc gaggcttatc cggcatgcaa tcttagcgga
     7501 aactgactgt ttcacccgct tgctgtgtcg ccgcctgttc cagcaaatcc cagaaggttt
     7561 cgccgctgcg atcgcaaatc cattcatcgc cttttaggtc aaaatggtag ccgccctgtt
     7621 tggttgccag ccacacctgg tgcagcggct cctggcggtt aataatgatt ttgctgccgt
     7681 tttcaaaggt aatggtcagt acgccgccgt tgatttcgca gtcgatatcg ctgtcgccat
     7741 cccagtcgtc caggcgttct tcaatggtca gccagagttg atcagccagg cgatgaaatt
     7801 cactgtcgtt cattgttgta tcctgtcttt aagtgatggc ggcagtatag cggtatgggg
     7861 tcagggcttc aaagtttgcc cgtctgttgc tgcgttccgg catgagtcat ccgtcaacgg
     7921 aataataatg tctctgttgt gaagttcacg cacattctct tctctgacgt gtgttttcgc
     7981 cgggcagggg gagggcgtaa tccttacctc tgtatcggtc catgtataaa cattgcgatc
     8041 gtcataagcg aagcgatcgt ttttagttag cggaccgtta actggaataa tttgcacgct
     8101 ctttgaccgt aattggcgga taaaataagc atgctgtttg ccgctattaa atgagcgatc
     8161 aatgaccagg tctggcgtat ccagcgggat tatctcaagc tggtcttcac caaatgtcga
     8221 taattttgtt acatacagcc gatctttata ttgcgcaata ttgccagtaa tcagatggaa
     8281 ttgttcaggt tcaatatctg gtaggtctat ccacttgctc cactgacggt cggacgtgag
     8341 ctgtttacgc cagcgaacgc gactttcccc tacttcaaaa gagacgtcat catctacggg
     8401 tatcgctttg ccagacaatt gcccaaaggg ataacgatgc atacccttat tgtcgcgaaa
     8461 aaccagcagt gaatgaggta tccagcgaat tatctcaaat gagtctgcat tcgccgtaag
     8521 gtgaacgcca tttaaataaa tattctgccc actgcggata agcagatcat tggaatacaa
     8581 atttctgttt acactcataa gacgtggatt aataccccac catctttttt ctaatacagt
     8641 aacattactg ctgctcccct gacgtcgacc ggacaaatag agatttttac tatccaccag
     8701 cgtggtagag ttaccttcga tagcttttag tgtcgccaga tcaacacgtg acgcgccgct
     8761 attactttca gtgcgctgtc cgtcaaaata gaggctatat ttatcgaccg cgaagcggcc
     8821 catagcctga aagctggcaa tatcgacagt gggtgttccc ggtggattgc taacgatttt
     8881 gccacgccag agaataacgc ggccgtctgt cacccagtgg tattcctcat tttttatgct
     8941 tatttcatcg gtgggtatat agccttccac caactcccgc tccccggata tatttgtcac
     9001 aaggcgcaaa tcggccctgc tcatctccgg aaactgggca atgattttct tatcccccca
     9061 ataaccttta ccgtaagaca taacaccgtg atcattgtaa tagtcgaacc ctatggcctg
     9121 tacagcaggc gtcagaaatg tgtgtcccaa caggatgagt gcaactgtga ttatttgacc
     9181 cttcattttg tcgcctcatt gcgcggggca ggaataaatt cgccgtcatc agtaattttt
     9241 ccgtccagat atcccgtttt gtaatttacg ccatagccat atcccgaatg ttgttccttc
     9301 cagattaagc gaacttgttt cggatcagcg gtgcggtatc gccagagctg cgcgccatca
     9361 aagctgtaaa tgtagcggtt atcgcgggca taccatctgc gcaaaaaggg atggtcatta
     9421 agatgggctt cgcgttcatc cggagaacgg ttatccagta agatcagcgg tccgtcagat
     9481 tcaaacacat gtaaaccgga atggtatggc cacccttcgg cacgcgtaag taaatagccg
     9541 tggcgtttcc cggcgttgaa acgtttgttg ataataagat tgggatcatc aagtttcacg
     9601 atttccagct tgcggtcacc agactctgtt tcgatgatgg tataaagcga gtcctgatac
     9661 tgggcgacag catcactgat gggttgaaac tgctccggat cgagtccggg taattcttcc
     9721 tgtttacaag caggtccttt acgccagaac actttctttt ccagcagaga aaaatccaga
     9781 cagtgctttg ttaaatcttc atcccactcc agattttcct tgtcgaggac tttacgctgc
     9841 agcccgtttt tatcacgcca ggtcaagagt gagccgggca tccagcgcac gacggaaaag
     9901 gtgtccgggt cggcatcaag ctgttcgccg ttgatcagga ttttggtccg tgtacgagcc
     9961 agggtatccc aggggccaaa tggcatagcc acacagggat taaacgcggt agaaaacttt
    10021 cctcgctgat cccatgactt ttgtgccagc acacggaaac tatcgggatc tgcgaggcga
    10081 tgcccattga tatacaagaa attagcgtca cgtaatatca gccccagtaa gtcaggatcc
    10141 cagggaggcc ggaattccac ctgatgcagt gttttgatat ctaagcgatt tcccccgcca
    10201 ttgttatcgg tgcgcttgcc ttcgaaatag agactgtgtt tatctgcggc aaaatcaccc
    10261 cacgccttga acgaggcgac atcgacggga ggtttgtctg gcgggttttg cacaacttca
    10321 cccgcgtaga ggatatagcg accatcgcta tgccaggagt aatgaggata ccaggcctgt
    10381 gcgccttcat agaggtagtc ggttgtcaga tcagtcagag gaggtacttt atcccatttg
    10441 tctagtgttg tcgggtctat taataaattg ggtaaacggc gcaaactttt gtaattcacg
    10501 tcaggaagag aaagatttgt ttctttataa gggcgcaggt ataccacgcc ttttccctgt
    10561 ttctggtaaa tattatgttc aggcggatca agccgacatc cccatgccga cgataaaaac
    10621 aatgaacaag acaacaaaca aaccacattg cgatagtgca taaagccatc ctggcgcgag
    10681 gtgccgatca cgaaactacc agcagaacat aattcctcgc acgtaagcgt aacttttgcg
    10741 gcaaatcgtc acttttcaga atctataaag gccatcgcta ttttctgata gttgctgggg
    10801 taaacttgag gttttcattc acgatataaa tacgagctac tgcgccatgg tgtgactgat
    10861 cacgttaaaa tgtgccggat tgacgccggg aagggtgtca acatgcgact ctgcccaccg
    10921 ttacctactt tattgtcttt cgcgtgttgt acttaggtcc caccaccggt atctaacatt
    10981 ccccacatta ttacgctttc tgccgaacgt gatttttata gaaatctatc aattcatttt
    11041 ccctgtacca taaagtttat ctttcacatc ctgccgaacg cgggtcttgt accccccgtt
    11101 gtgaagatct tcatacggag aaaaagaatc tttactccac cccagcagca ctttatcgcc
    11161 aggcataaaa tgcacatgca gaaaatcttc tccccattct cttttgggga gaggtaacgt
    11221 catattccgt ttctccagac gcattccagc attatactgt gtacgagtag tacttaatgt
    11281 ccagataacc tctgccgtat ccccactaac atcaccacaa caggttgttg aaccgccacc
    11341 atttgcaaaa gcattcgcgc ccgccacgcc gttaacactg aatcccagta tcgggcggtc
    11401 aatttcactg tggatttcca gaatcactcc acctcttggt cctccccata ctgcctgtcc
    11461 tatattccac aacacgacca gtaccggaac gacaattaac gtccgccata cccagcgccc
    11521 ccaccgggca taaccgcgcg taagcttttg gtcaaatttt tccagttggg taaatagccc
    11581 catcagattt tattccctct tatcaccagg ttggcaaagt tgtatgtcac tggcgactaa
    11641 ggtcagacaa caaaattgca tggtgcgcca ctcttttaaa agctccatgc cgacgataag
    11701 aacaacgaac aagccaacaa acaaactaca ttgcgatagt gcatagagcc atcctggcgc
    11761 gaggtgccga tcacgaaact accagcaaaa cataaatccc cacgagtaag cgttatactc
    11821 gcagcatttc ctcacttttc agacttcata aagagtcgct aaacgcttgc ttttacgtct
    11881 tctcctgcga tgatagaaag cagaaagcga tgaactttac aggcaatcca taatgaaaaa
    11941 cgtgtttaag gcactcactg tattaattac tctcttcagc ctgacgggct gcggtctgaa
    12001 aggtccgctc tatttcccgc ctgcagataa aaacgcaccg ccgccgacca aaccggtaga
    12061 gacgcaaacg caatccacgg tgccggataa aaacgatcgc gccactggcg atggtccatc
    12121 ccaggtgaat tactaaaagt cagtttctgt acccgcgtga ttggagtaaa tgatgcagtt
    12181 ctcgaaaatg catggccttg gcaacgattt tatggtcgtc gacgcggtaa cgcagaatgt
    12241 ctttttttca ccggagctga ttcgtcgcct ggctgatcgg cacctggggg tagggtttga
    12301 ccaactgctg gtggttgagc cgccgtatga tcctgaactg gattttcact atcgcatttt
    12361 caatgctgat ggcagtgaag tggcgcagtg cggcaacggt gcgcgctgct ttgcccgttt
    12421 tgtgcgtctg aaaggactga ccaataagcg tgatatccgc gtcagcaccg ccaacgggcg
    12481 gatggttctg accgtcaccg atgatgatct ggtccgcgta aatatgggcg aacccaactt
    12541 cgaaccttcc gccgtgccgt ttcgcgctaa caaagcggaa aagacctata ttatgcgcgc
    12601 cgccgagcag acaatcttat gcggcgtggt gtcgatggga aatccgcatt gcgtgattca
    12661 ggtcgatgat gtcgataccg cggcggtaga aacgcttggt cctgttctgg aaagccacga
    12721 gcgttttccg gagcgcgcca atatcggttt tatgcaagtg gttaagcgcg agcatattcg
    12781 tttacgcgtt tatgagcgtg gggcaggaga aacccaggcc tgcggcagcg gcgcgtgtgc
    12841 ggcggttgca gtagggattc agcaaggttt gctggccgaa gaagtacgcg tggaactccc
    12901 cggcggtcgt cttgatatcg cctggaaagg tccgggtcac ccgttatata tgactggccc
    12961 ggcggtacat gtctacgacg gatttattca tctatgaagc aaccagggga agaactgcag
    13021 gaaacactca cggagcttga tgaccgggcg gttgtcgatt atctgattaa aaatcctgag
    13081 ttttttatcc gtaatgcgcg cgcagtagaa gcgatacgtg tgccgcatcc ggtacgcggc
    13141 accgtttcgt tggtcgagtg gcacatggcc cgcgcacgta atcatattca tgttctggaa
    13201 gagaacatgg cgctgttgat ggaacaggct atcgccaacg aaggcctgtt ttatcgccta
    13261 ctctacctgc agcgcagtct caccgccgcc agcagtctcg acgatatgct gatgcgcttt
    13321 caccgctggg cgcgcgatct cggcctggca ggtgcgagtc tgcgcctgtt tccggatcgc
    13381 tggcgcttag gtgcgccgtc gaaccacact catctggcat taagccgtca gtctttcgaa
    13441 ccgctgcgta ttcagcgttt ggggcaggaa cagcactatc ttgggccgct taacggacca
    13501 gagctgctgg tggtgctacc ggaagcgaaa gcggtgggat cggtggcgat gtcgatgctg
    13561 ggaagcgatg ctgatttggg tgtcgtgctg tttaccagtc gcgatgccag tcactatcaa
    13621 caagggcaag gaacgcagtt acttcatgaa attgcgctga tgttgccgga gcttctggag
    13681 cgttggattg aacgcgtatg accgatttac acaccgatgt agaacgctac ctacgttatc
    13741 tgagcgtgga gcgccagctt agcccgataa ccctgcttaa ctaccagcgt cagcttgagg
    13801 cgatcatcaa ttttgccagc gaaaacggcc tgcaaagctg gcagcaatgt gatgtgacga
    13861 tggtgcgcaa ttttgctgta cgcagtcgcc gtaaagggct gggagcagca agtctggcgt
    13921 tacggctttc tgcgctacgt agcttttttg actggctggt cagccagaac gaactcaaag
    13981 ctaacccggc gaaaggtgtt tcggcaccga aagcgccgcg tcatctgccg aaaaacatcg
    14041 acgtcgacga tatgaatcgg ctgctggata ttgatatcaa cgatcccctc gctgtacgcg
    14101 accgtgcaat gctggaagtg atgtacggcg cgggtctgcg tctttctgag ctggtggggc
    14161 tggatattaa acacctcgac ctggagtctg gtgaagtgtg ggttatgggg aaaggcagca
    14221 aagagcgccg cctgccgatt ggtcgcaacg ctgtggcgtg gattgagcac tggcttgatt
    14281 tgcgcgacct gtttggtagc gaagacgacg cgctttttct gtcgaaactg ggcaagcgta
    14341 tctccgcgcg taatgtgcag aaacgctttg ccgaatgggg cataaaacaa gggctgaata
    14401 atcacgttca cccacataaa ttacgtcact cgttcgctac gcatatgctg gagtcgagcg
    14461 gcgatcttcg tggtgtgcag gagctactgg gtcatgccaa cctctccacc acgcaaatct
    14521 atactcatct tgattttcaa caccttgcct cggtgtacga tgcggcgcat ccacgcgcca
    14581 aacgggggaa ataatgcgtt tttaccggcc tttggggcgc atctcggcgc tcacctttga
    14641 cctggatgat accctttacg ataaccgtcc ggtgattttg cgcaccgagc gagaggcgct
    14701 tacctttgtg caaaattatc atccggcgct gcgcagcttc cagaatgaag atctgcaacg
    14761 cctgcgccag gcggtacggg aagtggaacc cgagatttat cacgacgtga cgcgctggcg
    14821 ttttcgttcg attgaacaag cgatgctcga cgccgggctg agtgccgaag aagccagtgc
    14881 aggcgcacac gcagcaatga tcaactttgc caaatggcgc agccgaatcg acgtcccgca
    14941 gcaaactcac gacaccttaa aacagctggc gaagaaatgg ccgctggtgg cgatcaccaa
    15001 cggtaacgcc cagccggaac tgtttggttt gggggattat tttgagtttg tgctgcgcgc
    15061 tggcccgcac gggcgctcaa aaccgttcag cgatatgtac tttctggctg cggaaaaact
    15121 caacgtgccg atcggcgaga tcttacatgt tggggacgat ctcaccactg acgtgggtgg
    15181 ggcaattcgc agcggaatgc aggcctgttg gatcagaccg gaaaatggcg atctgatgca
    15241 aacctgggac agccgtttac tgccgcatct ggaaatttcc cggttggcat ctctgacctc
    15301 gctgatataa tcagcaaatc tgtatatata cccagctttt tggcggaggg cgttgcgctt
    15361 ctccgcccaa cctattttta cgcggcggtg ccaatggacg tttcttacct gctcgacagc
    15421 cttaatgaca aacagcgcga agcggtggcc gcgccacgca gcaaccttct ggtgctggcg
    15481 ggcgcgggca gtggtaagac gcgcgtactg gtgcatcgta tcgcctggct gatgagcgtg
    15541 gaaaactgct cgccatactc gattatggcg gtgacgttta ccaacaaagc ggcggcggag
    15601 atgcgtcatc gtatcgggca gctgatgggc accagccagg gcggcatgtg ggtcggcacc
    15661 ttccacgggc tggcgcaccg tctgctgcgt gcgcaccata tggacgccaa tctgccgcag
    15721 gattttcaga tcctcgacag cgaggaccag ctacgcctgc tcaaacgcct aatcaaggcg
    15781 atgaacctcg acgagaagca gtggccgccg cgccaggcaa tgtggtacat caacagccag
    15841 aaagatgaag gcctgcgtcc acatcatatt caaagctacg gtaatccggt ggagcagacc
    15901 tggcagaagg tgtatcaggc gtatcaggaa gcgtgtgacc gcgcgggcct ggtggacttc
    15961 gccgagctgc tgctacgtgc tcacgagttg tggcttaaca agccgcatat ccttcagcac
    16021 tatcgcgaac ggtttaccaa tatcctggtg gacgaattcc aggataccaa caacattcag
    16081 tatgcgtgga tccgcctgct ggcgggcgat accggcaaag tgatgatcgt tggcgatgac
    16141 gatcagtcaa tctacggctg gcgtggagcg caggtggaga atattcagcg tttcctcaat
    16201 gatttccccg gtgctgaaac tatccgtctg gagcagaact accgctctac cagcaatatt
    16261 ctgagcgccg cgaacgccct gattgaaaac aacaacgggc gtttgggtaa aaaactgtgg
    16321 accgatggcg cggacggtga gcctatttcc ctctattgcg cttttaacga actcgatgaa
    16381 gcgcgttttg tggttaaccg catcaaaacc tggcaggaca acggcggggc gcttgccgag
    16441 tgcgccattc tctaccgcag taacgcccag tcgcgtgtgc tggaagaagc actgttgcag
    16501 gcgagtatgc cgtaccgtat ttacggcggg atgcgcttct tcgaacgcca ggaaatcaaa
    16561 gatgcgctct cgtatctgcg cctgattgcc aaccgtaacg acgacgcggc ctttgagcgt
    16621 gtggtgaata cgccaacgcg gggtattggt gaccggacgc tggacgtggt acgtcagaca
    16681 tcgcgcgatc gccagttaac actctggcag gcatgtcgtg aactgttgca ggaaaaagcc
    16741 ctcgccggac gtgctgccag cgccttacag cggtttatgg aactgatcga cgccttagcg
    16801 caggaaactg ccgatatgcc gctgcatgta cagactgacc gggtaattaa agactccggc
    16861 ctgcgtacca tgtacgagca ggagaagggc gaaaaaggtc agacgcgtat cgaaaactta
    16921 gaggaactgg tgacggcaac gcgccagttc agctacaacg aagaagacga agatttaatg
    16981 ccgctgcagg cgttcctatc ccatgcggcg ctggaagcag gcgaagggca ggcggatacc
    17041 tggcaagatg cggtacaact gatgaccatg cactcggcga aaggtctgga gttcccgcag
    17101 gtgtttatcg tcggtatgga agagggcatg ttcccaagcc agatgtcgct ggatgaaggc
    17161 gggcgtctgg aagaagaacg ccgtctggcc tacgttggcg taacccgcgc gatgcagaaa
    17221 ctgacgctga cctacgcgga aactcgccgt ctgtatggca aagaggttta ccatcgcccg
    17281 tcgcgcttta ttggcgagct gccggaagag tgtgtggaag aggtgcgcct gcgcgccacg
    17341 gtaagccgcc cggtcagcca tcagcggatg ggtacgccga tggtcgagaa cgacagcggc
    17401 tacaagctcg gccagcgcgt acgccacgct aagtttggtg aaggcaccat tgtcaatatg
    17461 gaaggcagcg gtgagcatag ccgtttgcag gtggcatttc agggccaggg tattaaatgg
    17521 ctggtggcgg cttatgcccg gctggagtcg gtgtaacgtt gccggatgcg gtgctgcgca
    17581 ccttacttgg cctaaaaaat cattcagatt caataaattg caacgtcatg taggccggat
    17641 agggcgttta cgccgcatcc ggcatctgcg ccatcttcag tatctgacac aaaactatcg
    17701 ttttaacctt tccgctctac ggaaatcatg gtgacaaaag gatagcgttg ccacgggatt
    17761 gccccgcctt tcatatacat atgtgaaatc gtgccatcaa gataaagcag ctgctcaacg
    17821 ttcagtttcg ctttggcata acaggcaaaa tcataaaaat ttgtcgcctg ctggctcaac
    17881 aaaaacacgg cattcccatg tttattaatc ccaacaccgt tacgaatttt acgtgaggcg
    17941 acgttgggat gaatacgcgg attaattaca ctgttttcca tcaacattgg ccctgactgc
    18001 accgcaaact gaatctcttt actggttttg aaggcatcca gacgaacgat gccgacttta
    18061 tctcccgcga cataaaacac gccgccagga cggataaaga aattcccttc acctgaagcg
    18121 agatttaacg ccaccttctg ctgaccgttt tcgatgtaca aaccgagcgg cgcatagctt
    18181 tcatcataga tgccgccgtt catcgccatc tgcacctgac cctgactatt catatccgcc
    18241 agcagagcat gtaaagttcc ccacgcttcg ccattggctt tttgccagta cattttcacc
    18301 cgctctgttt gaggattaac ggtatacgcc tgtacggtca gcgtcggatc tgagagtgcg
    18361 caatcatcag cggcaacagc aaacaaggga agtaaggtga gggtgagaaa aatccgtttg
    18421 agattcaagg tgatcattcc tttaccaatg agtagccgat gcgccattat aggtcctgga
    18481 tgttggattt ttttatcctg ttagcgacct tgacgagtac caaaaagcgc gaagttcaac
    18541 tattgttctg tggtgttctg ttgcgtgttg acggcaaaat tttgctggcg taacatgcgc
    18601 gcacgatcac tctaagagga cattcgcctt ggacacaccc agtagatact ggctcactat
    18661 cctgtcatcc aggatcaact cctaaggcta tccctttttg ctgatagcct tagcggttgt
    18721 cagcgacctc aatttttccc gtcgcgctga gtcaggctgt ttaatggtct gaaacccaat
    18781 ttgtttctgt gtgcccaccg aactgtccga tattttaagc attgggagtc ccggtcatgc
    18841 tgagcgcatt tcaactggaa aataaccgac tgacccggct ggaagtcgaa gagtcacaac
    18901 cccttgtaaa tgcagtatgg attgatcttg tcgaaccgga cgacgacgag cgactgcgcg
    18961 tacaatctga acttggccag agcctggcaa cccgcccgga actggaagac atcgaagcat
    19021 cggcacgttt ctttgaagac gacgacggcc tgcatattca ctccttcttc ttctttgaag
    19081 atgcggaaga tcacgccggt aactccactg tggcatttac catccgtgat ggtcgtctgt
    19141 ttactctgcg tgagcgtgaa ctgcccgctt ttcgtctgta tcgtatgcgt gcccgtagcc
    19201 agtcgatggt agacggtaac gcctacgagt tgctgctgga tctgttcgaa accaaaatcg
    19261 aacagttggc agatgaaatt gaaaatatct atagcgacct ggagcagttg agccgggtga
    19321 ttatggaagg gcatcagggc gatgagtacg acgaggcgct ctccacgctg gcggaactgg
    19381 aagatatcgg ctggaaagtt cgcctgtgtc tgatggatac ccagcgcgcg ctcaacttcc
    19441 tggtacgcaa ggcgcgttta ccgggtgggc aactggagca ggcgcgtgaa atcctgcgag
    19501 atatcgaatc cctgctgccg cataacgaat ccctgttcca gaaggtgaac ttcctgatgc
    19561 aggcggcgat gggctttatc aacatcgagc agaaccgcat catcaaaatc ttctcggtgg
    19621 tatccgtggt gttcctgcca ccgacgctgg tggcttccag ctacgggatg aactttgaat
    19681 ttatgccaga gttgaagtgg agtttcggct acccaggcgc gattatcttt atgatcctcg
    19741 cgggcctggc accgtatctg tactttaagc ggaagaactg gttgtaatat gagtgccgga
    19801 taaagctatt tttttatccg gcttatttct aaaaattgtc ttttttaaag tcatgcggat
    19861 tgaaaactcg ctctatttta ttttcttgtg ttaattgata ctcagtaaaa tcgttaatac
    19921 caatatgagt gttacgataa gggtattcat taataaccat taagtaattt ttcacgatgc
    19981 aattatcatt gctcttaatt gtcgggaaac agtagagatc ttcatacggc ccttcgatat
    20041 atttttttat agtcagtgaa gccattaccg atatcaatga cataccaggt atgccaacta
    20101 ccttctggta ggggatgttg atttttaatt tcattctgat gcttcagata ccaatttatt
    20161 ttatcgctgt cagtccatgg cagatgatca acgaccagaa agactgaatt gccagcatca
    20221 gagtatgtat acaaaatctg tacaggacgc aatgtccata aattat
//
LOCUS       DAZFWL020000083        19473 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFWL020000083 DAZFWL020000000
VERSION     DAZFWL020000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.083"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            129..2339
                     /gene="katP"
                     /locus_tag="ACHKNP_RS15740"
                     /old_locus_tag="pgaptmp_003148"
     CDS             129..2339
                     /gene="katP"
                     /locus_tag="ACHKNP_RS15740"
                     /old_locus_tag="pgaptmp_003148"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HGU4705273.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            2383..2772
                     /locus_tag="ACHKNP_RS15745"
                     /old_locus_tag="pgaptmp_003149"
     CDS             2383..2772
                     /locus_tag="ACHKNP_RS15745"
                     /old_locus_tag="pgaptmp_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU4705274.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            2886..3653
                     /locus_tag="ACHKNP_RS15750"
                     /old_locus_tag="pgaptmp_003150"
                     /pseudo
     CDS             2886..3653
                     /locus_tag="ACHKNP_RS15750"
                     /old_locus_tag="pgaptmp_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    3166..3276
                     /locus_tag="ACHKNP_RS15750"
                     /old_locus_tag="pgaptmp_003150"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(3733..4128)
                     /locus_tag="ACHKNP_RS15755"
                     /old_locus_tag="pgaptmp_003151"
     CDS             complement(3733..4128)
                     /locus_tag="ACHKNP_RS15755"
                     /old_locus_tag="pgaptmp_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGU4705275.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDNLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIG
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            complement(4128..5087)
                     /locus_tag="ACHKNP_RS15760"
                     /old_locus_tag="pgaptmp_003152"
     CDS             complement(4128..5087)
                     /locus_tag="ACHKNP_RS15760"
                     /old_locus_tag="pgaptmp_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HGU4705276.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNITHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5360..6262
                     /locus_tag="ACHKNP_RS15765"
                     /old_locus_tag="pgaptmp_003153"
     CDS             5360..6262
                     /locus_tag="ACHKNP_RS15765"
                     /old_locus_tag="pgaptmp_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGU4705277.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTYLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWYQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSHP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            6259..6570
                     /locus_tag="ACHKNP_RS15770"
                     /old_locus_tag="pgaptmp_003154"
     CDS             6259..6570
                     /locus_tag="ACHKNP_RS15770"
                     /old_locus_tag="pgaptmp_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705278.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            6646..7329
                     /locus_tag="ACHKNP_RS15775"
                     /old_locus_tag="pgaptmp_003155"
     CDS             6646..7329
                     /locus_tag="ACHKNP_RS15775"
                     /old_locus_tag="pgaptmp_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HGU4705279.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWHRIDRFMAVWIRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNSIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNRFEPEAA"
     gene            7329..7547
                     /locus_tag="ACHKNP_RS15780"
                     /old_locus_tag="pgaptmp_003156"
     CDS             7329..7547
                     /locus_tag="ACHKNP_RS15780"
                     /old_locus_tag="pgaptmp_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705280.1"
                     /translation="MNYSGFEDMREQVAELANTMCDLRTTMNEMEQRYSDADTLPERL
                     VRQTLFRASHLFMKAYNEILELDYCFKD"
     gene            7507..7993
                     /locus_tag="ACHKNP_RS15785"
                     /old_locus_tag="pgaptmp_003157"
                     /pseudo
     CDS             7507..7993
                     /locus_tag="ACHKNP_RS15785"
                     /old_locus_tag="pgaptmp_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            8038..8277
                     /locus_tag="ACHKNP_RS15790"
                     /old_locus_tag="pgaptmp_003158"
                     /pseudo
     CDS             8038..8277
                     /locus_tag="ACHKNP_RS15790"
                     /old_locus_tag="pgaptmp_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8272..8520
                     /gene="psiB"
                     /locus_tag="ACHKNP_RS15795"
                     /old_locus_tag="pgaptmp_003159"
                     /pseudo
     CDS             8272..8520
                     /gene="psiB"
                     /locus_tag="ACHKNP_RS15795"
                     /old_locus_tag="pgaptmp_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
     gene            8517..9236
                     /locus_tag="ACHKNP_RS15800"
                     /old_locus_tag="pgaptmp_003160"
     CDS             8517..9236
                     /locus_tag="ACHKNP_RS15800"
                     /old_locus_tag="pgaptmp_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HGU4705281.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            9233..9830
                     /locus_tag="ACHKNP_RS15805"
                     /old_locus_tag="pgaptmp_003161"
                     /pseudo
     CDS             9233..9830
                     /locus_tag="ACHKNP_RS15805"
                     /old_locus_tag="pgaptmp_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10292..10792
                     /locus_tag="ACHKNP_RS15810"
                     /old_locus_tag="pgaptmp_003162"
     CDS             10292..10792
                     /locus_tag="ACHKNP_RS15810"
                     /old_locus_tag="pgaptmp_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGU4705282.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPVNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(10794..11414)
                     /locus_tag="ACHKNP_RS15815"
                     /old_locus_tag="pgaptmp_003163"
     CDS             complement(10794..11414)
                     /locus_tag="ACHKNP_RS15815"
                     /old_locus_tag="pgaptmp_003163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705283.1"
                     /translation="MDLSDGAATATARGSCPGADAKVFDACGKKFCGWYIFPWTAAPC
                     CGRFSPRKVHSGKQAAGVAAEGLTAPSGAVALHTVRCAGSAVSGATGAARPERSLQAW
                     FFRHRTVAAARNRASGFGGDWRVRQQLPVGVGPTTGCRQAAGSRRVSPPPGIRTTETT
                     KQKNASHHDGQEGQRRRRGPPVFQAGVSLPGFSGGPAGHTPAGAGE"
     gene            11520..11954
                     /locus_tag="ACHKNP_RS15820"
                     /old_locus_tag="pgaptmp_003164"
     CDS             11520..11954
                     /locus_tag="ACHKNP_RS15820"
                     /old_locus_tag="pgaptmp_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HGU4705284.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKSAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            12047..12313
                     /locus_tag="ACHKNP_RS15825"
                     /old_locus_tag="pgaptmp_003165"
     CDS             12047..12313
                     /locus_tag="ACHKNP_RS15825"
                     /old_locus_tag="pgaptmp_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705285.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENIPLHRHTRVRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERACH"
     gene            12378..13268
                     /locus_tag="ACHKNP_RS15830"
                     /old_locus_tag="pgaptmp_003166"
     CDS             12378..13268
                     /locus_tag="ACHKNP_RS15830"
                     /old_locus_tag="pgaptmp_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGU4705286.1"
                     /translation="MSKKKNTTTPTPHDAAFWSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEEPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGALEKALAIACQLQKMGMT
                     PEQIKQATGLSEAELKNITH"
     gene            13268..13513
                     /locus_tag="ACHKNP_RS15835"
                     /old_locus_tag="pgaptmp_003167"
     CDS             13268..13513
                     /locus_tag="ACHKNP_RS15835"
                     /old_locus_tag="pgaptmp_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705287.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKKGALEV
                     AREAACRLRDMGWTPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(13544..13795)
                     /locus_tag="ACHKNP_RS15840"
                     /old_locus_tag="pgaptmp_003168"
     CDS             complement(13544..13795)
                     /locus_tag="ACHKNP_RS15840"
                     /old_locus_tag="pgaptmp_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4705288.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(13829..14024)
                     /locus_tag="ACHKNP_RS15845"
                     /old_locus_tag="pgaptmp_003169"
                     /pseudo
     CDS             complement(13829..14024)
                     /locus_tag="ACHKNP_RS15845"
                     /old_locus_tag="pgaptmp_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14374..15222
                     /locus_tag="ACHKNP_RS15850"
                     /old_locus_tag="pgaptmp_003170"
     CDS             14374..15222
                     /locus_tag="ACHKNP_RS15850"
                     /old_locus_tag="pgaptmp_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4705289.1"
                     /translation="MNQTLPTADLNTAGTTDIIPSMAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTYTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQELIDIWRKLSW
                     DYRTNNPCCLGKRIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNIGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     VLPPRV"
     gene            complement(15308..15643)
                     /locus_tag="ACHKNP_RS15855"
                     /old_locus_tag="pgaptmp_003171"
     CDS             complement(15308..15643)
                     /locus_tag="ACHKNP_RS15855"
                     /old_locus_tag="pgaptmp_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HGU4705290.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            15875..16207
                     /gene="mobA"
                     /locus_tag="ACHKNP_RS15860"
                     /old_locus_tag="pgaptmp_003172"
     CDS             15875..16207
                     /gene="mobA"
                     /locus_tag="ACHKNP_RS15860"
                     /old_locus_tag="pgaptmp_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HGU4705291.1"
                     /translation="MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRDLMGR"
     gene            16219..18939
                     /locus_tag="ACHKNP_RS15865"
                     /old_locus_tag="pgaptmp_003173"
     CDS             16219..18939
                     /locus_tag="ACHKNP_RS15865"
                     /old_locus_tag="pgaptmp_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HGU4705292.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEI
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELEIKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLATAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     TDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ITRPDVDLHRQRSEQYYREYIRQQTRLSVSHDDNYTLRQEKTWEPPSPGM"
     gene            complement(19160..>19473)
                     /locus_tag="ACHKNP_RS15870"
                     /old_locus_tag="pgaptmp_003174"
     CDS             complement(19160..>19473)
                     /locus_tag="ACHKNP_RS15870"
                     /old_locus_tag="pgaptmp_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HGU4705293.1"
                     /translation="KLRLPHPQYDTDIESRANELWKKAVHTINNTTREERRVCYITLN
                     RPEEEHSDPEDIPSVKAILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEK
                     Y"
BASE COUNT         4648 a         4661 c         5340 g         4824 t
ORIGIN      
        1 tgcggcttgg aaccccttag tattttttgt ctgtagtatc tatcccagca ataggtatat
       61 cctgttgcat caataaagtt gacttttgta tacaacatgc gaatttccct taatccggag
      121 ctattcgtat gataaaaaaa actcttcctg ttctgattct tctggcgcta tcggggagct
      181 tttctaccgc tgtagccgct gataaaaaag agactcaaaa tttctactat ccagaaacac
      241 tggatttaac tcctctgaga ttacacagcc ctgaatcaaa tccctggggg gctgattttg
      301 attatgccac cagatttcaa cagctggata tggaggctct gaaaaaagat atcaaagatt
      361 tgctgacaac ttcccaggat tggtggcctg cggattatgg tcattatggt cctttcttta
      421 ttcgtatggc ttggcacggt gccggaacat acaggacata tgatggccgg ggaggcgcca
      481 gtggtggtca gcaacgtttt gaaccgctga acagctggcc ggataacgtt aatctggata
      541 aagcccgtcg attgctgtgg ccagtcaaga aaaaatacgg ctccagtatt tcctggggag
      601 acctgatggt cctgactggt aatgttgccc ttgaatccat gggatttaaa acgctgggat
      661 ttgctggcgg aagagaagat gactgggagt cggacctggt atactggggg cctgacaaca
      721 agcctcttgc agataaccgg gataaaaacg ggaaacttca gaaacctctt gccgccacgc
      781 agatgggact tatttatgtc aatcctgaag gccccggtgg aaaaccagat cctctggctt
      841 ccgcgaaaga tatcagggaa gctttttcac gtatggccat ggatgatgag gagactgtgg
      901 ccctgatcgc gggagggcat acatttggta aagcacatgg tgcagcgtct cctgaaaaat
      961 gtattggcgc agggcctgat ggtgcacctg tggaggagca gggactggga tggaaaaata
     1021 aatgtggtac aggaaacggc aaatatacca tcaccagtgg cctggaagga gcctggtcga
     1081 catcgccaac ccagttcaca atgcagtatc tgaagaattt atataaatat gaatgggagc
     1141 tgcacaagag tcctgccggt gcttatcagt ggaagcctaa aaaagcggca aatatagttc
     1201 aggacgcgca tgatccgtct gtcctgcatc cgttgatgat gtttacgacg gatattgctc
     1261 ttaaagttga tcctgaatat aagaaaataa ccacccgttt cctgaatgat ccaaaagctt
     1321 ttgagcaggc attcgcaaga gcatggttta aactgaccca ccgggatatg ggaccggcag
     1381 cccgatatct tggtaatgaa gttcctgcag aatcatttat ctggcaggat cctcttcctg
     1441 cggcggatta tacaatgatt gatggtaaag acattaagtc gctgaaagag caggttatgg
     1501 atttgggtat ccctgcatct gagctgataa agacagcctg ggcttcagct tccacatttc
     1561 gtgtgactga ttatcgtggg ggaaataatg gtgcccgcat caggttacag cccgaaatta
     1621 actgggaagt taatgagcct gaaaaactga agaaagtact ggcatccctg acctcattac
     1681 agcgtgaatt taacaaaaaa cagtctgacg gaaagaaagt gtcgttggct gatttaattg
     1741 ttctttcggg taatgctgca atcgaagatg cggccagaaa agccggggtg gaacttgaga
     1801 ttccctttac tccgggaaga actgacgcct ctcaggagca gacggatgtt gcctcattca
     1861 gtgtactgga gccgacagca gatggattca gaaattatta ctcaaaaagc agaagtcata
     1921 tatcgccggt tgaaagcctc attgataaag ccagtcagct ggatctcacc gttcctgaaa
     1981 tgacggcatt actgggtggt ctgcgggtaa tggatattaa tacaaataat tcttcgttgg
     2041 gagtgtttac cgatacccct ggtgttctgg ataacaagtt ttttgttaat ctgctggata
     2101 tgtcaacacg atggagtaaa gcagataaag aagatacata caatggattc gatcgtaaaa
     2161 cgggagcatt aaaatggaaa gcatcctctg ttgatttaat cttcagttca aatcctgaat
     2221 tacgtgcggt ggcagaagta tatgcctcgg atgatgcgag aaataagttt attcatgatt
     2281 ttgttaaatc gtggaataaa gttatgaata gcgatcggtt tgatttaaac aataaatgat
     2341 aaaactgatg agggagggca cctccctcat gaaggagaaa ttatgcagaa aagcaataaa
     2401 gttataatca gctgtggtat ggcattatat tttttgtgtg gggggattac agtggcagaa
     2461 gaacttgatg aaagcatgca agttcttgca aggaatatga gtgtacttca gtcatcaatg
     2521 gataaaaagg agcttcttga agctcttgac agaatggatg atgctgtaga tgagtcaatg
     2581 aagcgtcttc ctgagagcat ttcaccggct gatgaagaag ggaagaatga ctatataaaa
     2641 gaacttcaaa agcttgaaaa agagattatg ctggcgaaac aaaaagtctt atcgggacag
     2701 ttatcagata tacccgattc ggtaaacaag atgaataaaa tcagagttga ggggcatgat
     2761 aaattcagat aaatcagttg gttgattcct gcgggacgaa tacatacgtc aggaacataa
     2821 ctttatggca gtgaaccgcc ccgggaatcc tggagactaa acttcctgag aaagaggtaa
     2881 acaggatgac taaaaatact cgtttttccc ccgaagtccg tcaacgggcg attcatatgg
     2941 ttctggaaag tcagggcgaa tatgactcac aatgggcggc aatttgttcc attgcccaaa
     3001 gattggctgt acaccggaga ctctgcgtgt ctggggacgc cagcatgagc gggataccgg
     3061 gagcggtgat ggtgggctca ccagcgctga acgtcagcgt ctgaaagagc tggaacgtga
     3121 aaaccgtgaa ctgcgccgca gtaacgatat ccttcgccag gcttccgctt attttgcgaa
     3181 ggccgagttc gaccgcctct ggaaaaaata atgccactgc tgctgcgtga gcagtacggg
     3241 gtcggaccgg tatgcagtga actgcatatt gccccgtcaa cgtattacca ctgtcagcaa
     3301 cagcggtatc atccttataa acgtagtgcc tgtgcacagc gcgatgactg gctgaagaaa
     3361 gagatactgc gcttatacga tgagaatcat caggtatacg gtggcgtaaa gtctgacgtc
     3421 agttgttaca ggaaggtatc agggtggcca gatgtatagt ggcgcgcctc atggcggtta
     3481 tgggacttgc cggtgttctc cggggtaaaa aggtccgtac taccgtcagc cggaaagccg
     3541 tttccgcagg cgaccgcgta aaccgtcagt tcgtggcaga acgtcctgac cagctgtggg
     3601 tggctgattt tacttacgtc agcacatggc agggcttcgt ctgtgtggcg ttcgatttag
     3661 ggcactaaaa ccgctgctgg tccctcataa taaaagaaga gcaggtaata gacttaacct
     3721 gctcttcttt tattattttt ttaacataga aagattgcgt ctggtttgtt cttcgaaatc
     3781 atcttttgaa gtgatgctct ctgatttgtt ttctgaagat gcccctatga tgttttttaa
     3841 cacacttata tcaattgatg tagatgtgta tcctgttgtt tgctctataa gtttcaccag
     3901 attttctgca gtgattttct cactgaaaaa accactgatt agcgttaaca gtcgggggtc
     3961 aacagaataa agagctgctc cgcaaataag agattgtctg tagaagtctc cccgcatttg
     4021 tatcgggaga gagtctattg tttgttgtgt caggttatcc tgcggataaa gtgtcgggtg
     4081 caggtatgcc acaactttct ttcttttttt cggatcactg ctttccatta cgcatgcacc
     4141 attgctttca ccaatgcaaa ctgaggttca tccaccttgt tcactttcag attcgggaaa
     4201 tattgcttta tgtgagggta aataagctcg gctcctccgc cagtgagata gattcggttc
     4261 attcctgaga atgttgaaat ttcctgcctg atgctttctg ccagagattt cacttccctg
     4321 tctattacat gagtaatgtt ttttatttca gctacatcat ttattaatgt ctggagatat
     4381 tcattatcat gtctgttttt tattaactca tcagcaataa aataattaca tggtgtggag
     4441 gctttcgtca gcgcattcat gacggtatga gtgatgcggg atgtcccgat ttctgaatac
     4501 ccacggattt cagaaatgcc ttcaaatgca ccgagaatta aaccacaatc cagagtggtt
     4561 cctccaagat cgataatcag gctgcgttca agtttgttta ctttatcttt ttttagtgat
     4621 tcaaatgcag ctggcaaaga ttcaggcatt acattaactt ttttaatttt aaatgtttcc
     4681 cctttattca gagatatctc acggagaaca tttgcctttt ttctttctat tctttcttca
     4741 tttggttgat tgtcattatc aaagaattcg gttacaggta atgtcactgt cagttctaca
     4801 tcctgtggct gaaggccgga tgtcagcagt gcatgatgta tggcaagcaa attggtggtg
     4861 ctgtattgat aactgacatg tgttgtacct atgacagcag tactgcccaa atcataggtg
     4921 tatttttttc catccacgac gtaattgtaa actttgccgg cattaaacag tcccgggttc
     4981 cagccttctt taaagctgtt tcctgacaga ctggttctta cctcgccatc ttcaagccag
     5041 gccagtttta cgttagttga accatcatcg catgatactt ttagcatttt ttcttgcctc
     5101 gttttgagtt tttatttatc tcaaattgag catcctttat ctagatgcat ctttttgagt
     5161 tttttgttgt tccttattgt gtctcatttt gggttcatgt caagcgatga ggctctttta
     5221 gagtctcttt gcattcttgt tgtgactcag tttgagtctc tatcgatttc tcattttgcg
     5281 tttcgaactg agacggaaac tacttaacca ggccgcatgt gagaggagaa ttcccatgcc
     5341 aaactggtgc tcaaatcgta tgcatttttc tggtgaaccg gcacagattg ctgagattaa
     5401 acgactggcc agcggtgccg tcacaccgct ttatcgccgc gccacaaatg aaggtattca
     5461 gctgtttctg gccggaagtg ccggacttct gcagaccact gaagatgtgc ggtttgaacc
     5521 atgccccgga ctgacggctg ccgggcgtgg cgttgtatcg ccggagaata tcgcgttcac
     5581 ccgctggctg acatacctgc agaacggtgt actgctggat gagcaaaact gcctgatgct
     5641 gcatgaactc tggctgcaga gcggtactgg acagcgtcgc tgggaaggat taccggacga
     5701 tgtcagggat accatcaccg cacttttcac cgcaaaaaga ggtgactggt gtggcttctg
     5761 gagtaacgag gatgtatcgg tgtggtggaa ccgtctgtgt gacaacgtac tgccggaaaa
     5821 aaccatgccg tttgacctgc tgacggttct gccgacccgc ctggatgttg aagtgaatgg
     5881 ctttaacggt ggtgttctga acggtgttcc ttctgcatat cactggtata cggaacagta
     5941 tggcgtgaag tggcctgtgg ggtatgaggt gaatatcagt agtcagggag acaacttcat
     6001 tcaggttgat ttcgacacgc cgtggtatca gccggaaagc gacgttattg cagaattaag
     6061 ccgccgtttc agctgcacac tggagcactg gtatgccgaa cagggctgca atttctgtgg
     6121 ctggcagcga tatgagcgtg gcgagcttgt tgatgtgctg tggggggagc tggaatggtc
     6181 acatccgaca gatgatgacg agttgccgga agtcaccgga cctgcgtgga tagtcgacaa
     6241 ggtggcgcat tatggcggat gaagtatgac ggagacgggc gggcaactgc ccgtttcttt
     6301 tccggtaaag gatgtcgccg tgctcctctt tttactgcgc cggctgacgc ggcgcggcat
     6361 aaacgctgtc tgtggtcagt gtcccgcgtc cggcgggcac gggacgggcg cttttaccgc
     6421 tcccggctgg cgtcggtgac agtgtacgcc agcccgtcgc ccttttctga tgaacgccct
     6481 tcaagccgct ttcgcggcat agccttgccg tcaaaaagac gaaggctgcg gtattccacg
     6541 gtcggcctga cccgttaccg gacgcggtga acagcccaca ggcagcgggt aacgggcacc
     6601 acaggggtgc cctcccggtg ccctctgtaa gagaaggagt tttgtatgtc ccgttttgtc
     6661 ctcggtaact gcatcgatgt tatggcccgt attcctgata acgccattga tttcatactg
     6721 actgacccgc cgtacctcgt cggtttccgt gaccgttccg ggcgcaccat tgccggtgac
     6781 gttaatgatg actggctgca acctgccagc aatgagatgt accgcgtact caaaaaagac
     6841 gcgctgatgg tcagcttcta cggctggcac cgtattgacc gctttatggc ggtctggata
     6901 cgcgccggat tcagcgtggc agggcacctc gtgttcacca aaaactacac atccaaatct
     6961 gcgtatgtcg cgtaccgcca cgaatgcgct tacatcctgg caaagggcag tccggcccgg
     7021 ccccgtaacc cgctgccgga tgtgctgggc tggaaatatt cgggcaaccg ccatcacccg
     7081 acggaaaagc ccgttaccag cctgcaaccg ctgattgaga gcttcacaca cccgaactca
     7141 attgtgctgg acccgtttgc aggcagcggt tcaacctgcg tggccgccct tcagtccgga
     7201 cgccggtata ttggtatcga gctgcttgaa cagtatcacc gtgccggaca gcaacgcctt
     7261 gccgccgtgc aacgggccat gcagcagggg gccgcaaatg ataacaggtt tgaaccggag
     7321 gcagcatgat gaattacagc ggatttgaag atatgcgtga acaggtggct gaactggcca
     7381 acaccatgtg cgacctgcgc acaaccatga atgagatgga gcagcggtac agcgatgcag
     7441 acaccctgcc ggaacgtctg gtgcgtcaga cgctctttcg tgccagccac ctgtttatga
     7501 aggcatataa cgaaatactg gaactggatt actgcttcaa agattgaggg gaaagagtat
     7561 gtacggaaca tgcgaaacgc tctgccgggc gctggcagca aagtatccgg gagacacgcc
     7621 gttgatgctg gttatctggt ccccggaaga gattcaggcc ctcgctgacg gaatggatat
     7681 ttccctggcc gatcatgaaa tcaggaccat cctggcgcgc ctggaggaca tcccggaaga
     7741 ccagcggact gagtccggta tttcttcctc cgcggcgatg gagattatca gcaacgtgag
     7801 cgaaaaccgc caggtgaccg tccctgctga actgctggcg tccctgattc agaccgctga
     7861 acaggcattg tggaagcgtg aatgggccgc ccgtgattac ggcctcgccg tcccggaatg
     7921 cgttacccgc cgtcaggcgg tggttaatca ggcccgcatc ctgctgaaaa acaacacgca
     7981 cgaaaacgac tgatgttatc gccgcctccg ggcggcgatt cagggagacc atgaatcatg
     8041 aataaaacgc tgaatgcact gatttgtcgt catgcccgcg acctgctgct ggcgcagggc
     8101 tggccggaag agacggatgt tgaccagcgt aacctgaagt atccgggctg gatcagcatt
     8161 tacgtgcggc tggatgcgcc ccggctggcg acgttactcg ttaaccgcca cgatggcgta
     8221 ctgccgccgc acctggcctc cgccattcag aaactgaccg gaaccggggc ggaacttggt
     8281 ttcacgccag cccacgaacg tttccacctg gcattatgtt cgccgggaga cgtctctcag
     8341 ctctggatgc tggttctggt gaattgtggt ggacagcctt tcgccgtcgt tcaggtgcaa
     8401 catatcttca cgcctgtcgc tatcagtcac acgctggcgc ttgccgcgac acttgatgcg
     8461 cagggataca gtgttaacga catcatccat atcctgatgg cagaaggagg tcaggcatga
     8521 gcgcacgttc acgggcactg attcccctca gcgcagaaca acaggccgcc atgcaggctg
     8581 tggctgtcac agaacaacgt cgtcgtcagg gacgcacact ttcagcatgg ccttatgcca
     8641 gcgctttctt tcgctgcctg aatggcagtc gccggatttc gctgaccgat ctccgctttt
     8701 ttgcccctgc gctgacgaag gaggaatttc atggcaaccg tctcctgtgg ctggctgccg
     8761 tggataaact gattgaaagt tttggtgaag tctgtgttct tcccctgcca tccgatgcgg
     8821 ggcatcgtct gttcccgtcc gttccttttc gtgaaggtga gcgtcgtcgt cagaaaacca
     8881 cgctgacaga gcagaaatac agccgccagc gggaacgtga ggcagaacga cgggaactgg
     8941 aataccagac atgttttgct caggcgcaga ttgaccttgc gtttcatact cccgccacgg
     9001 tcggaagctg gttgtcccgc tggtctggtg ttgttgagga gcatgatctg gaaacgattt
     9061 tctgggggtg gtgcgggcgt tttccatcac tgtcatcatt tgaccggttt ttctggcagg
     9121 aggaaccact ctggcggctg atttttgaag ccggtgaggc cggtcgtggt gcaccggtac
     9181 aggtacgtgc acttgagcag tggatgatcc cgaacaagct ggagaacgca atatgatgaa
     9241 atcagacgaa caatatcagg ttcccgtgtg gatgcgacct ctgttgccgt tgctctgcaa
     9301 taccggtggt aatgatccgg aggaacttct gaatgataca gaaaccaccg ccagtgcgaa
     9361 tattgtccgt tatgtactga tagttgcagt gcggtcgcag attggatctg ctgcagctac
     9421 tgtacaggaa aggactgttg cgcacagaga taccaggtgg cttttcaccg gaagaagcgc
     9481 aggaactcct ggataatctg gtgcgcagcc atatcagcaa ggcgctgtcg ggtgagcgaa
     9541 tggcagcccg tgacagatat gccgagctgg cctggattcg ccagcaactg gtggatgccg
     9601 cctggtttgt ccgtgccaca ctggaagcgc atggtacgag cgtcggaaat gagagtccct
     9661 ctgcacttcc agagacaatg ccggacatac agacacggga actggtgatg ctgatcaaac
     9721 gactggcatc ttctctgaaa gcggtgaaac cggacagtag cgtggtgcgt gcggcgcagg
     9781 actggctgtg cgacagaaaa cttgtggata tcacagatat tctccggtga accaggcatg
     9841 tatatttttc gcagggacac gccgatcatg atcgtttcat atcaggggtg gtctggaggt
     9901 catgcggcgt gtcctctgca ctcgccggaa taaggaagtc gccggcggat ccgcttttac
     9961 ccggccatgc ggggcatggc cttgtgggtt ttcagttatg tggcctcagc gtcgtgtgcg
    10021 ggctgtgccg tgcctccatc gtagccgggc ttctgcgggc gggatgcaag gtaagatgaa
    10081 ccgctgcggt cacccggtcc ctccttcccg tctgccgtga ttttccggtc gttcacccgc
    10141 tcagatttcg cggtctcacc ctccaactcc cgtcagccgt gcgcgtcggg ctgcggcttc
    10201 ccttgcgccc ttgcatccct gcaggcccgg caaagatgga ggcacggcac cgcccggtgt
    10261 cgcagaacat gtgactatgg aggattcggg aatgtctgtt gttgcacctg ctgtatacgt
    10321 tggaacctgg cataaataca actgtggaag catcgctggc cgctggtttg acctgaccac
    10381 gtttgatgat gaacgcgact ttttcgccgc ctgccgtgct cttcaccagg atgaagccga
    10441 tcctgaactg atgtttcagg attatgaggg gttcccggtg aatatggcct ccgaatgcca
    10501 tatcaactgg gcctgggttg aaggcttccg ccaggcacgg gatgaaggct gcgaagaggc
    10561 ttatcgtctc tgggtggatg ataccggtga gacggatttt gacaccttcc gcgatgcctg
    10621 gtggggcgag gctgacagtg aggaggcttt tgcggttgag ttcgtcagtg ataccggcct
    10681 gctggctgac gtgccggaga cggtggcgct ctattttgac tatgaggcgt atgcgcggga
    10741 tttattcctg gactccttca cctttattga cggtcatgtg ttccgtcggt gatttactcc
    10801 cccgctccgg cgggggtatg gcctgccggg ccgccggaaa acccaggcag gcttacgcct
    10861 gcctgaaata caggagggcc gcgcctgcgg cgctgccctt cctgcccgtc atggtgtgag
    10921 gcatttttct gttttgtcgt ttctgttgtt ctgatgcccg gaggcgggga cacccttcgg
    10981 ctcccggctg cctggcggca tccggtcgtc ggcccgactc cgacaggcag ctgctggcgc
    11041 acccgccagt ctccgccgaa cccgctggcg cggtttcggg ctgccgctac ggtccgatgt
    11101 ctgaaaaacc aggcctgcag cgaccgttcc ggtcgcgccg cccctgtcgc gccggaaacg
    11161 gcgctccctg cacacctgac ggtgtgtaag gccactgcgc ctgatggcgc agtcaaccct
    11221 tctgccgcca cccctgcggc ctgtttcccg ctgtggactt tccgggggct gaagcggcca
    11281 caacacgggg cagccgtcca ggggaagatg taccagccac aaaacttttt gccgcaggcg
    11341 tcaaaaactt ttgcgtctgc ccccggacaa ctgccccgtg ccgtggcagt cgctgcccca
    11401 tcggaaaggt ccactgcggg gagaaacccc gaagaagtgg acggcagaag gggctttcgc
    11461 ccctccgggg gagcaaccgg tcggcccggc gacctgattc agcagaagga gacgttttca
    11521 tgacagtcag cagcacgatt tccgtttttt gccgcgacgg ggtgttccgt accgtttact
    11581 gccacctgca cggggagccg acctggaacg gtcgcatcct gcatacccac tatgccaccg
    11641 gtcagctggc cgaagccctg gttgaacacg gagatatccg ttgcctcggt ccccgttgcg
    11701 acaaatccgc cgggcatacg cttcagaacc cggtggacgg tgtgacggct tattacggac
    11761 gtgacagtgg tttccggatg gacagtgagg cgcgtgagta ccgttctttc atggaggcta
    11821 ttgccactga aagcactgaa gaggtgcgct tccattatgt gttcatcgac ggctactgga
    11881 aggtgatgta ccgcacgccg gaaggctgga agatgaaagc gctcgcgctg gcactgcgcc
    11941 gctgtccgaa atgaaaaaag gcagggcgat aaaccctgcc tttctccgcc ggcgcttccc
    12001 cgtacggaag tccggcatct caatcactat ctatggagat tatgccatga gaccatccat
    12061 tatcttcgca accgccgagt atgtaaagcg tctgcgtgaa gagtgcctgc gggagaatat
    12121 acccctgcac cgccataccc gcgtcagacg tcaggagctg gcacaggatg agattaaccc
    12181 ggacgtcctg gcgatgagcg gtcatatcgc cagacgctgc agtgagcaga agcgggtacg
    12241 tatccccgcc atgaaagtca gcgaatgggg ccacctgctc cgcgcgcttg aaattgagcg
    12301 ggcctgccac tgattaaccc ttgccgtctg cctgtgcagg cggcaggata cgttcagaca
    12361 tctcaccagg aggaaccgtg agtaagaaga aaaacaccac aacgcccacg ccgcatgatg
    12421 ccgcgttctg gtcgttcctg gcgaatcccg acgtcgccag agattttctg gaactgcatc
    12481 ttccggcgga gtaccggcag ttgtgcgacc tgtccacgct gaagctggaa cccgccacct
    12541 ttgttgagcc ggacctgcat cagtacgcca gcgatatcct ctggagtgtg aaaaccaccg
    12601 ggggtgaaga cggctatgtt tatacgctca tcgagcacca gagcaccgaa aatctgtaca
    12661 tgcctttccg catgttacgt tacagtgtgg cggcgatgca gagacatctg gagcagcaca
    12721 aaacattgcc actggtgatt ccggtgctgt tctatcatgg tgagcgcagc ccgtacccgt
    12781 acagcatgaa ctggctggac tgttttgagg aaccggcgct tgcggctaaa atatacacaa
    12841 agccgtttcc gctggttgat atcactgtcg ttgatgacaa tgaaatcatg aaccatcgcc
    12901 ggatggccgc actgacgctg ctgatgaagc atatccgcca tcgtgacatg atggagctgc
    12961 tggacaaact cccgcaggtc atggtggaaa tttcagacga gcaggtgcgt gttctgattc
    13021 attacatcgt taacgcaggg gactctgtat caccggaatt tatgcgggcg ctggctgagc
    13081 gtctgccgca gcatgaggat aaactgatga ctatcgctga acgtcttgag caaaaaggtc
    13141 gccaggaagg agcgcttgaa aaagccctgg ctattgcgtg ccagcttcag aaaatgggga
    13201 tgacgccgga gcagattaag caggctaccg gactttccga agctgaactg aagaacatca
    13261 ctcactgatg agaaccacaa agcccggcaa aatgccgggt ttttttgtca gcaggagcag
    13321 agcaaacagc atgacgattg cagaacgtct tatacagaaa ggtttcgacg aaggttttaa
    13381 gaaaggtgcg cttgaagtgg cgcgggaagc agcctgccgg ctgcgggata tgggctggac
    13441 gccggaacgg attcaggagg caaccggact ttccggtgaa gaactgaaaa agctgtttcc
    13501 tgatgagcag tagcctggca ttctgccagg ctgtgaatca cgctcacaca ttccctgccg
    13561 tttcccccag ttcattttcc agacgcctga tgatatctgc cttgcgcatc ccctgcatgt
    13621 gaggcagcag atcacggact gaaagtgcct gggtggtcct ccagacggtg acatccacct
    13681 gttccgcatt ttcccaggcg tgccatgttc tctgtgtcac gccaaattct ctggccgctt
    13741 tttcgcgtga ccagagcatg ctttttcgcc agattcgcag ttcccagccg gtcataaaac
    13801 acctttgaaa aagtgaaaaa acttcacttt attttatcat cataatcacc aggtcagcgc
    13861 gataaataag agtgaaaaag tgaagaaatt tcacttttta gttgtaggtg tcgtgctgtt
    13921 ttctctgttt tcacctgtgc gggagtggtg cgccagtgac caccacatgc cattgttgtg
    13981 ctgtcgtttt tcttcagtcg cgccggatgc ggcgctccct gcacacctga cggtgtgtaa
    14041 ggccactgcg cctgatggcg cagctgtctc tgcctgtctg ccgccgcact gcggcccgtt
    14101 ctccgcagac ggactttccg ggggtacata cacgaccaca tcccgtttca gtcgtccagg
    14161 ggagagatgt accagccaca aaactttttg ccgcaggcgt caaaaacttt tgcgtctgcc
    14221 cccggacaac tgccccgtga cgtggtgagc gtcccccatc ggaaaggtcc actgcgggaa
    14281 gaaccccctg tgaagtggac ggcagaagga gcttcgctcc tccgggaaat gaccaggctg
    14341 acccggcagc cgtgacgatg aggagacaat atgatgaatc agactttacc cactgctgac
    14401 ctgaatactg ccggcacgac agatattatt ccgtctatgg ctatcgaccg catcatcgcg
    14461 cagcgtaacg aaggtattgc actgttcatg caggcgatgg aatgcctggc gacagcgcgc
    14521 aggattctgc tcgatgcttc cggtgacatt tttctctacg ggtttgaaga ctgcgtgact
    14581 gactccgttc gatgcatgga taaaccggaa gaagcaaaaa agaatatcac ccgtcttgcc
    14641 gaccggaaaa tctgggatcg cctgatgact tatacgggca tgtacacctt catgagttca
    14701 tgccagcgtg atgagtggaa cagccagctg atgagcgaca cctgtcctga aatcaccctg
    14761 gacaatgtac tggcaacatt ccgccatctg aatgccagta agatgcagac atttgaacag
    14821 gaactgattg atatctggcg gaaattgtca tgggattaca gaaccaataa tccctgctgt
    14881 ctgggcaaga gaatcattat tgaaaacctg ctgtaccgct ggagtaacgg gcgtgtgacg
    14941 ctggactgca gcggacggga ggcactggat gacctggtac gtccgtttta tctgctggag
    15001 ggacgcaacg ttcctgactt caggaacagc atcggggcgc agtatggtga atttctcggg
    15061 aacggcgaca atatcggtga gttgtttgaa ggggaatatt ttacggtgcg tggctaccag
    15121 aaagggaccg tacacattgt ctttaagcgt cctgaccttg ttgaaaaact gaatgatatt
    15181 attgcacggc attatcctgg tgtattgccg ccacgagtct gattaaacag aaaagccctg
    15241 gtatttatgc cggggctttt ttttctgtaa tcatcataat ctcagaatac gatgtgacat
    15301 tgtcacatca attgttgttc atatcgatgc catctttcct gcctgaatct gtctttttag
    15361 gtgctgtttt agaggaagaa cggcgggatg gttttttcga ttctgcttcc cgaataatgt
    15421 cacgaagtgc cttctctcct acttcatacc ctgcattttt gagcgtgtca cgaacatctg
    15481 ccagtgtata accctttgtc cttaccagga caatgatgtc atcacgaatg gcatcaagaa
    15541 aatcacgcaa tgtttttcgt tgagcggtga ggtccggcaa ttctgataaa gcagcttttg
    15601 ccagctggat gtcatcatct gagtaaaatt ttttagaagc cattggcact ttttctccgt
    15661 ttcatgttga agtctgattt tatcatcaaa aactgcctcc tggcagagct gcgccccgcc
    15721 tctgccgtct gcataagact atgatgcaca aaaataacag gctataatgc ctgaaaaacg
    15781 ggacaggatg tgtaatcgta taaccgcgca cgcacggcta tacgattacc acctggtcag
    15841 ggcttagccc cgacaccccg gtaaggagca gaccatgagt gagaagaaaa cccgaagtgg
    15901 cagtgagaaa cggcaaaaga atgtcctcat tgctgttcgt ttttcaccag aagaggctga
    15961 gattgttaaa gaaaaggcag aaaaaaacgg actgacagtt tcaactctga taagaaaaac
    16021 tgtgctggga aaacagataa atgcccgcat tgacgaagat tttttgaaag agctgatgcg
    16081 tcttggtcgc ctgcagaaac atctgtttgt ggaaggaaaa cgcacgggag ataaagagta
    16141 tgcagaggtt ctcgttgcta ttacagagct tgctaatact ctgcgaagag atttaatggg
    16201 acgctgagat aatcgctgat gaatgccatc attccgaaaa aaagacgcga cggaaagtct
    16261 tcttttgaag acctgattgc gtacacctca gtgcgggatg atgtgccgga agatgaactc
    16321 aggccagaca cggaaatgaa agctgagatt ccgcaccgga accgcttcag acgtcttgtt
    16381 gattatgcaa cacgtctgcg taatgaaaaa tttgtttccc tgattgatgt gatgaaagac
    16441 ggcagtcagt gggtgaattt ctatggtgtg acctgctttc ataactgttt gtctcttgaa
    16501 tctgcggctg aagaaatgca gaaagcagca gaccttgctc atttttccaa agatgataca
    16561 gatcctgttt ttcactatat actctcctgg cctgcgcacg aaagtccgcg ttctgagcag
    16621 ctttttgact gtgtgcgtca caccctgaaa tcccttgagc tgtcaaaaca tcagtatgtg
    16681 gcagctgttc atacagacac ggataacctg catgtacatg ttgcagtgaa tcgtgtccac
    16741 cctgaaaccg gctatatcaa ttgccttccg tggagccagg aaaaactcag tcgtgcatgc
    16801 cgtgaacttg aaattaagca cggattcgcg ccggataatg gctgctttgt gcatgcaccg
    16861 ggcaatcgta ttgtgcgcaa aacagcgctg gtacgtgagc gccgtaatgc ctggcgacgc
    16921 gggaaaaagc agacgttcag ggaatatatt gttcagatgt cgattgccgg gctgcgtgaa
    16981 gagcccgcac aggactggtt atctttgcat aaacggctgg ccagtgatgg tctgtatatc
    17041 acgatgcagg aaggggaact ggtggtgaag gatggctggg acagagcgcg ggaaggtgtt
    17101 gcactcagtt cgttcggacc gttatggaca gctgaaaaac tggggcggaa actgggggaa
    17161 tatcagcctg taccgacaga tattttcagt caggtgggga cacccggtcg ttatgatccg
    17221 gaagccataa acgttgatat ccggccggaa aaagtggcag aaacggagag tctgaaacag
    17281 tacgcctgcc gtcattttgc cgaacgctta ccggaaatgg cgagaaatgg tgagctggaa
    17341 agttgcctgg atgtgcacag aactctggca acggctggat tatggatggg gatccagcac
    17401 gggcatctgg tactgcatga tggatttgat aaacaacaga caccagtacg tgcagacagt
    17461 gtgtggccgt taatgacgct ggattatatg caggatctgg atggtggatg gcagcctgta
    17521 ccgaaggata tttttactca ggttataccc ggagaacggt tccggggacg taatcttggt
    17581 actcaggctg tcagtgatta cgaatggtat cgtatgcgga tggggacagg tccacaggga
    17641 gcaataaagc gcgaactgtt ttctgataag gaaagtctgt ggggatacgg ccttattcag
    17701 tgcaggagcc tgattgaaga gatgatagct gacggggatt tcagctggca ggcatgtcat
    17761 gagatgtttg cgcgtaaagg gcttatgctg cagaaacagc atcatggtct tgtgattgtg
    17821 gatgcgttta atcatgagct gacaccggtg aaggccagca gtatccatcc tgatttgacg
    17881 ttatcccgtg ccgagcctca ggccgggcca tttgaaattg cggctgcaga tattttcgaa
    17941 cgggtgaagc ctgaatgtcg ttacaatccg gaacttgctg ccagtgatga agtggaacca
    18001 ggcttccggc gcgatcctga gattcgtcgt gagcgccgtg aagcccgtgc agctgcgcgg
    18061 gaagatttgc gtgcgcgtta tcttgcatgg aaggagcact ggcgtaaacc cgacttacgt
    18121 tacggagaac ggttacggga aattcatgct gcctgccgtc ggcgcaaggc gtatatccgt
    18181 gtgcagttcc gggatccaca attgcgtaag ctgcattacc atattgcgga agtgcagcgt
    18241 atgcaggcgc tgatcaggct gaaggagagt gtgaaggaag agcggttgtc actgattgcg
    18301 gagggtaagt ggtatccttt gtcctaccgg cagtgggtgg aacaacaggc ggctcaggga
    18361 gacagggctg cagtatcgca gttacgtggc tgggattatc gtgatcgtcg ctcgcgtaat
    18421 aaggataaaa gacgcacaac taatgttgat cgttgtgtcg tactctgcga acccggagga
    18481 acgccacttt ttaataatgt tgcaaaactt gaagctcgtc ttcagaaaaa tggaagtgtt
    18541 catttcaggg atacgcggac aggaaaaaat gtctgtacgg attacggaga tcgggttgtg
    18601 ttctaccatc acacggaccg aaatgaactg gcagagaagc ttgatttaat tgcgccagtt
    18661 ctgttcagcc gcaacggaaa gcttggtttt gagccggaag gttcatacca gcagtttaat
    18721 gatgtgtttg cagaaatggt ggcctggcac aatgcagcgg gaataaccgg aaacggacac
    18781 ttcaccatta ctcgtccgga cgtggatttg caccgtcaac gcagtgagca gtattaccgg
    18841 gaatatatca gacagcagac ccgtttatcg gtgagccatg atgataatta tacgttacgg
    18901 caggagaaaa catgggagcc accatcacct ggaatgtaag cccggataag tgatggtgca
    18961 aagattcagc ccaaaaacgg caggaacctt cgtcaataag ggcgggagta gtcactgaat
    19021 tgagtttata cccggttaac tctgaattaa gggccgtgtc tgcggccctt atccagtgcg
    19081 ccgtaaaacc ccggacttag tgaacagggt tttacagtct gattgacaga tatggtcacc
    19141 agaaaatatt tgtattcctt cagtattttt cgtcagttgt tggttgtgct ccacatgatt
    19201 tctggggatg atcattattt attgtctgcg atttattatt ttcagcgacc ggtaacagta
    19261 tctccagggg gagcagggta ttcagaatcg ccttcactga aggtatatct tcaggatcag
    19321 agtgttcctc ttccggacga ttcagtgtga tataacatac ccggcgttct tccctggttg
    19381 tgttattgat agtgtgcaca gcctttttcc acagttcgtt tgcccgggat tcgatatctg
    19441 tatcatattg cggatgaggt agcctgagtt taa
//
LOCUS       DAZFWL020000084        19338 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFWL020000084 DAZFWL020000000
VERSION     DAZFWL020000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.084"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            88..738
                     /gene="csgD"
                     /locus_tag="ACHKNP_RS15875"
                     /old_locus_tag="pgaptmp_003175"
     CDS             88..738
                     /gene="csgD"
                     /locus_tag="ACHKNP_RS15875"
                     /old_locus_tag="pgaptmp_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HGU4705294.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            743..1132
                     /gene="csgE"
                     /locus_tag="ACHKNP_RS15880"
                     /old_locus_tag="pgaptmp_003176"
     CDS             743..1132
                     /gene="csgE"
                     /locus_tag="ACHKNP_RS15880"
                     /old_locus_tag="pgaptmp_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HGU4705295.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1157..1573
                     /gene="csgF"
                     /locus_tag="ACHKNP_RS15885"
                     /old_locus_tag="pgaptmp_003177"
     CDS             1157..1573
                     /gene="csgF"
                     /locus_tag="ACHKNP_RS15885"
                     /old_locus_tag="pgaptmp_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HGU4705296.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1600..2433
                     /gene="csgG"
                     /locus_tag="ACHKNP_RS15890"
                     /old_locus_tag="pgaptmp_003178"
     CDS             1600..2433
                     /gene="csgG"
                     /locus_tag="ACHKNP_RS15890"
                     /old_locus_tag="pgaptmp_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HGU4705297.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2497..2988)
                     /gene="ycdZ"
                     /locus_tag="ACHKNP_RS15895"
                     /old_locus_tag="pgaptmp_003179"
     CDS             complement(2497..2988)
                     /gene="ycdZ"
                     /locus_tag="ACHKNP_RS15895"
                     /old_locus_tag="pgaptmp_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGU4705298.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGSFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3090..3644)
                     /gene="ycdY"
                     /locus_tag="ACHKNP_RS15900"
                     /old_locus_tag="pgaptmp_003180"
     CDS             complement(3090..3644)
                     /gene="ycdY"
                     /locus_tag="ACHKNP_RS15900"
                     /old_locus_tag="pgaptmp_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HGU4705299.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3668..4405)
                     /gene="ycdX"
                     /locus_tag="ACHKNP_RS15905"
                     /old_locus_tag="pgaptmp_003181"
     CDS             complement(3668..4405)
                     /gene="ycdX"
                     /locus_tag="ACHKNP_RS15905"
                     /old_locus_tag="pgaptmp_003181"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HGU4705300.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4460..5398)
                     /gene="ghrA"
                     /locus_tag="ACHKNP_RS15910"
                     /old_locus_tag="pgaptmp_003182"
     CDS             complement(4460..5398)
                     /gene="ghrA"
                     /locus_tag="ACHKNP_RS15910"
                     /old_locus_tag="pgaptmp_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HGU4705301.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGADVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5632..5719
                     /locus_tag="ACHKNP_RS15915"
                     /old_locus_tag="pgaptmp_003183"
     tRNA            5632..5719
                     /locus_tag="ACHKNP_RS15915"
                     /old_locus_tag="pgaptmp_003183"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5666..5668,aa:Ser,seq:gga)
     gene            complement(5869..6711)
                     /locus_tag="ACHKNP_RS15920"
                     /old_locus_tag="pgaptmp_003184"
     CDS             complement(5869..6711)
                     /locus_tag="ACHKNP_RS15920"
                     /old_locus_tag="pgaptmp_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4705302.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6796..6993)
                     /locus_tag="ACHKNP_RS15925"
                     /old_locus_tag="pgaptmp_003185"
     CDS             complement(6796..6993)
                     /locus_tag="ACHKNP_RS15925"
                     /old_locus_tag="pgaptmp_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4705303.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7005..7496)
                     /locus_tag="ACHKNP_RS15930"
                     /old_locus_tag="pgaptmp_003186"
     CDS             complement(7005..7496)
                     /locus_tag="ACHKNP_RS15930"
                     /old_locus_tag="pgaptmp_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4705304.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7493..7867)
                     /locus_tag="ACHKNP_RS15935"
                     /old_locus_tag="pgaptmp_003187"
     CDS             complement(7493..7867)
                     /locus_tag="ACHKNP_RS15935"
                     /old_locus_tag="pgaptmp_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4705305.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7914..8288)
                     /locus_tag="ACHKNP_RS15940"
                     /old_locus_tag="pgaptmp_003188"
     CDS             complement(7914..8288)
                     /locus_tag="ACHKNP_RS15940"
                     /old_locus_tag="pgaptmp_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4705306.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(8451..8672)
                     /gene="yeeT"
                     /locus_tag="ACHKNP_RS15945"
                     /old_locus_tag="pgaptmp_003189"
     CDS             complement(8451..8672)
                     /gene="yeeT"
                     /locus_tag="ACHKNP_RS15945"
                     /old_locus_tag="pgaptmp_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU4705307.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8735..9211)
                     /gene="yeeS"
                     /locus_tag="ACHKNP_RS15950"
                     /old_locus_tag="pgaptmp_003190"
     CDS             complement(8735..9211)
                     /gene="yeeS"
                     /locus_tag="ACHKNP_RS15950"
                     /old_locus_tag="pgaptmp_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGU4705308.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9227..9712)
                     /locus_tag="ACHKNP_RS15955"
                     /old_locus_tag="pgaptmp_003191"
     CDS             complement(9227..9712)
                     /locus_tag="ACHKNP_RS15955"
                     /old_locus_tag="pgaptmp_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4705309.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9803..10621)
                     /locus_tag="ACHKNP_RS15960"
                     /old_locus_tag="pgaptmp_003192"
     CDS             complement(9803..10621)
                     /locus_tag="ACHKNP_RS15960"
                     /old_locus_tag="pgaptmp_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4705310.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(10961..12030)
                     /locus_tag="ACHKNP_RS15965"
                     /old_locus_tag="pgaptmp_003193"
                     /pseudo
     CDS             complement(10961..12030)
                     /locus_tag="ACHKNP_RS15965"
                     /old_locus_tag="pgaptmp_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
     gene            complement(12027..12932)
                     /locus_tag="ACHKNP_RS15970"
                     /old_locus_tag="pgaptmp_003194"
     CDS             complement(12027..12932)
                     /locus_tag="ACHKNP_RS15970"
                     /old_locus_tag="pgaptmp_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HGU4705311.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            complement(12929..15313)
                     /locus_tag="ACHKNP_RS15975"
                     /old_locus_tag="pgaptmp_003195"
     CDS             complement(12929..15313)
                     /locus_tag="ACHKNP_RS15975"
                     /old_locus_tag="pgaptmp_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HGU4705312.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRRAALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTSDLGW
                     ENYDEDVSRSVININKAREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            complement(15424..18273)
                     /gene="cah"
                     /locus_tag="ACHKNP_RS15980"
                     /old_locus_tag="pgaptmp_003196"
     CDS             complement(15424..18273)
                     /gene="cah"
                     /locus_tag="ACHKNP_RS15980"
                     /old_locus_tag="pgaptmp_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HGU4705313.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            complement(18645..>19338)
                     /locus_tag="ACHKNP_RS15985"
                     /old_locus_tag="pgaptmp_003197"
     CDS             complement(18645..>19338)
                     /locus_tag="ACHKNP_RS15985"
                     /old_locus_tag="pgaptmp_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4705314.1"
                     /translation="VTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDAEY
                     EALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPCHE
                     WDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDH
                     AASPLMTRLQDELCTESVRGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDTVV
                     SVARAVWNWIFF"
BASE COUNT         4527 a         5203 c         4834 g         4774 t
ORIGIN      
        1 cagtgcaaca tctgtcagta cttctggtgc ttctatttta gaggcagctg tcaggtgtgc
       61 gatcaataaa aaaagcgggg tttcatcatg tttaatgaag tccatagtat tcatggtcat
      121 acattattgt tgatcactaa accgtctttg caagcgacag ctctcttgca gcaccttaaa
      181 caatcgctgg caattacagg aaaattacat aatattcaac gttctctgga cgatatatct
      241 tcaggctcta ttattcttct ggatatgatg gaagcagata aaaagcttat ccattattgg
      301 caggatactt tgagcaggaa aaacaacaat atcaaaatat tgttgctaaa tacgcctgaa
      361 gattacccgt accgcgacat tgaaaactgg cctcatatca acggcgtttt ttatgccatg
      421 gaggatcaag aacgtgttgt caatgggttg caaggcgtcc tgcgcggcga atgctacttt
      481 acgcaaaagc ttgccagtta cctgattaca cattctggta actatcgtta taacagcacg
      541 gaatcagccc tccttactca tcgggaaaaa gagatcctga ataagctgcg tatcggcgcg
      601 tctaataacg agatcgctcg ttcgttgttc atcagcgaaa atacggttaa aacgcatctt
      661 tataatcttt tcaagaagat agccgtaaaa aaccggacac aagcggtttc ctgggcaaac
      721 gataacctca ggcgataaag ccatgaaacg ttatttacgc tggattgtgg cggcagaatt
      781 tctgttcgcc gcagggaatc ttcacgccgt tgaggtagaa gtcccgggat tgttaactga
      841 ccatactgtt tcatctattg gccatgattt ttaccgagcc tttagtgata aatgggaaag
      901 tgactatacg ggtaacttaa cgattaatga aaggcccagt gcacgatggg gaagctggat
      961 cactataacg gtcaatcagg acgttatttt ccagactttt ttatttccgt tgaaaagaga
     1021 cttcgagaaa actgtcgtct ttgcactgat tcaaactgaa gaagcactaa atcgtcgcca
     1081 gataaatcag gcgttattaa gtacgggcga tttggcgcat gatgaattct aaataaaaaa
     1141 ttgttcggag gctgcaatgc gtgtcaaaca tgcagtagtt ctactcatgc ttatttcgcc
     1201 attaagttgg gctggaacca tgactttcca gttccgtaat ccaaactttg gtggtaaccc
     1261 aaataatggc gcttttttat taaatagcgc tcaggcccaa aactcttata aagatccgag
     1321 ctataacgat gactttggta ttgaaacacc ctcagcgtta gataacttta ctcaggccat
     1381 ccagtcacaa attttaggtg ggctactgtc gaatattaat accggtaaac cgggccgcat
     1441 ggtgaccaac gattatattg ttgatattgc taaccgcgat ggtcaattgc agttgaacgt
     1501 gacagatcgt aaaaccggac aaacctcgac catccaggtt tcgggtttac aaaataactc
     1561 aaccgatttt taagccccag cttcataagg aaaataatca tgcagcgctt atttcttttg
     1621 gttgccgtca tgttactgag cggatgctta accgccccgc ctaaagaagc cgccagaccg
     1681 acattaatgc ctcgagctca gagctacaaa gatttgaccc atctgccagc gccgacgggt
     1741 aagatctttg tttcggtata caacattcag gacgaaaccg ggcaatttaa accctacccg
     1801 gcaagtaact tctccactgc tgttccgcaa agcgccacgg caatgctggt cacggcattg
     1861 aaagattctc gctggtttat accgctggag cgccagggct tacaaaacct gcttaacgag
     1921 cgcaagatta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa ccgaatcccg
     1981 ctgcaatctt taacggcggc aaatattatg gttgaaggtt cgattatcgg ttatgaaagc
     2041 aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga cacgcaatac
     2101 cagctcgatc agattgccgt gaacctgcgc gtcgtcaatg tgagtaccgg tgagatcctc
     2161 tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg ggttttccgc
     2221 tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa cgaacccgtt
     2281 atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa tgatggtatc
     2341 gaccgtggtc tgtgggattt gcagaacaaa gcagaacggc agaacgacat tctggtgaaa
     2401 taccgccata tgtcggttcc accggaatcc tgacagataa aaaagccgga attatcatcc
     2461 ggcattttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt tgatttcctg
     2521 ctcacggtga gccgtctttg cactacgcgc cgccagccac aggccgctgt ttttcattgc
     2581 gtaaccaaat accagcccaa gcgccagcga aggtaacacc agtttccagt caccctgccc
     2641 ggcaaacgtc gcgcatgcgc ctataaacga ccccggaaca aatgaaagca gcagctgttt
     2701 ggcctgaata cacatcagaa acgcgacaat accggttatg acataaccga gaatttccag
     2761 atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc cactaagcag
     2821 ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaggcaa agtaggccgt
     2881 acagcccagg aagcccgccc agcttagtaa gcccagggaa acagccaccc atccccagat
     2941 accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc cgcgcatgat
     3001 agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa caaattatct
     3061 gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc cagctcgtcc
     3121 cacattgcac taatggcatc acgggttagc ggtgccatgg tgcgccagaa aggcgtggtt
     3181 gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata ctcactgaac
     3241 agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca ggaagccgca
     3301 agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtggcatccc tcgttcggaa
     3361 agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg atatggcggc
     3421 acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg cgccatatca
     3481 cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt cgcagccagt
     3541 ttcccctcac gaatcagggt aaacagcggc accagtaaag gatcttgcgg ttggcggtaa
     3601 tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat atttccatta
     3661 ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat tcaaggaagt
     3721 tcagtaagcg gcgcggcgaa acattcaaaa tgcgctctgg cggaaaatct accgcgtcga
     3781 ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa tccgagccta
     3841 atgccaccca acctccagca tcacgtaccg ctgcggctac cgcacgacag ttgtcttcac
     3901 tgcccttacg tgagtgtaaa aatgaagaat tattgatttc cagcgccacc tgatgtttcg
     3961 cggctgcttc agcaacggct ttcacatcta tttcatattt gggatttccg ggatggctta
     4021 ttatatgcac attgccgctg gcgatagtgg cgatcatcgc ttgtgtattg gtcgctttgt
     4081 catgtggtgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc gagtcaaaca
     4141 ttttaccgct gcaatcaatt tcaccatcaa catttttaat gttagcttcg atgccgcgca
     4201 ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag tgccagtgat
     4261 gcggagcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta atgccctttt
     4321 gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg gcaacggtat
     4381 gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg ccaaggatag
     4441 caggaatcct gatgctttat tagtagccgc gtgcgcggtc gacttgcccg cagaccctct
     4501 cccctttttc gagctgggca atggtgcgag aaatgtactc cacagcttca gcgggacggg
     4561 taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc ggactttcag
     4621 gcggtaaggg ttcacgatta aaaacatcca acattgcgcc tttaactttg ccgctatcca
     4681 gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg aggagatacg
     4741 cgccatccgg taatttttcg agtaattgtt gattaataat gccgacggtt tcaggggtat
     4801 tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt tcttcccgtc
     4861 cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa cgcagcggaa
     4921 agcgccaggt ttgcagactc tgagcaactt tactgcccag tacgcctgcg cccaaaatgc
     4981 cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa ctattttgct
     5041 ggatgcgata atcgtcaaaa cgtcgaaacc aatgcagcac ctgactgaca gcatattcct
     5101 gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa gggttcagca
     5161 tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac atcggccccg agtgcgaaca
     5221 ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact aaagcataat
     5281 cagcagagtc attatctccg cttttccacg ctctgactct tgcctgagga atagctttgc
     5341 gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg atatccatac
     5401 tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat ctacaaaggc
     5461 caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt attaaaacca
     5521 cacattgatt gaagtttgaa taaacgcgcg attttttcaa aaagtttgtt gacctcaggt
     5581 catgatttcc ctaaattagc gcccgttcca gcaagacagg aacgacaatt tggtgaggtg
     5641 tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat cgggggttcg
     5701 aatccccccc tcaccgccag attataagcg catgttattg tttaacatgc gcttttttat
     5761 tttcccttcc agacagtatg ccatcgggta tgccatctga aaccggttta tatgccccgc
     5821 gatcatatct tatcggacaa tttccgatct ttgagcatac tggcaaagct atactgccag
     5881 catatcagga tagtgttttg cgataatatc attcagtctg tcaaccagtt ctggttttct
     5941 gaacgtgata tgcgcggagc ccttcttaaa gtatctgatg ctgaacatct catcttcata
     6001 gctctcttta ccctgaacag actgaatgtg atcatccaga cgaagggtga tgtttccccg
     6061 gttgtcgggg attggtttac cgctgaacag gtgcaacatt ctttccagat cggcgagtcg
     6121 gtcagcctgt tgtccggtga taagatgaaa tccccaccgg tcccatctca ccagattgtt
     6181 gacgataatt ttactgccaa atttacaggg gcaattggtt ttgtaattcc agttcagccc
     6241 tctgaagacg ttgatcaccc ctcgctcaaa cacctcatcc ttattctggt gcagttgttc
     6301 aaatgtgctc agaatgttcg cttcgctgat ttccggaaaa ttatcgtact ccagtgaccg
     6361 gtaccatgta tcacgggcct gcgtatccat taatgacagc atcccggaac gttgcatcag
     6421 gtcacgccag atttcgcgat caagattccg ggtaatggct ttcattgctg tttcgggttt
     6481 ctccatcagc cagcagccgc agcgaaaatc ctgtttcatg gcccagtccg gggcagtttt
     6541 acctccgatg ctttccgtca gcaccgagat ttcagccagt tgtcggataa gtgtgtcgat
     6601 ttgcaacagt gtactctttc gtacagccag aatgtggcga atgctggttg agcagataac
     6661 gtccgtatgc ccgttgacca cttccggacg ggagattgtg atatctgaca taatcgatat
     6721 tcctttttgc agataaatga aaacgccagc cctgtgcagg ctggcgtcag aggaatgaag
     6781 tctggtgagt gagcttcacc ggttctggtg cagaagctgc agatgacgca gggtacggac
     6841 atcctcccgg gcctgttcga tacagggcaa cagtgctgct gaatccgttt tatcctcatc
     6901 gttgtcaaag ataattcccg atccgcagtc gatattgtcc tgcagccacg caatcagaat
     6961 atccagcgct gtttccgtgg ttaatgattt catgttgttc ctggttacca gtcgaaagtg
     7021 ggtaaacccg gcaggcattc agcgctggca tccagatgaa tgagactgac gccataacgc
     7081 cggatgagtg tgacgaccag acgacggaac gtttttgaca gccccaggcg tttaagccgc
     7141 agaaccgggt atgaccacgc atcggtacgt aacagatatc cgctacctgt aaaatgaatc
     7201 cattctgatt caccgaagtc actggtctgg tgtgacagcg agtacaacca ggcgttgtcc
     7261 gtttccgtga tatgtgcggt actgcagcgt atgccggtaa gggtagcaaa tggtggcgtg
     7321 cattctgctg aggtctggtc agattcatca acaacacgaa gggagtagcc gttatcgcag
     7381 accatgttaa tcagttcagc gagctcaata ccatcgacgt caacgacaat gcgccccatg
     7441 ttcagtgcct gtacgttaac gctgtcggct tccagtgtca gggccagttt catcatttcg
     7501 cctccggata cttacccaag gtaatgttat ttaccgttct gtaattgtcg cgggtcatca
     7561 ggccggtcgc ccggcgagcc cggaggatat caatactgtt tattaactga gagcggggac
     7621 aggtgctgaa tcccggctgg tcggtacgca ccagcgcgta tttttccacg agaaaattaa
     7681 ctgcatcaca cagtgaaatg cctgcctcaa tatgctgctc aatcacacgt tcatcggcaa
     7741 acggtgtgtc attcagtgtg aggccataat gctggtccag cagtcggatg agcagtgtct
     7801 gccagatggt gacgggagac gggcagcgag atacctcccg tacatgtgtg tcggataatg
     7861 ttttcatctt aagttctctg taactggtag tgctaaaggt gagagcttcg gggttatgag
     7921 gtggttgcga gtgctggtgt cggataaaca gccagataaa cgtaaccaca ggagccgagg
     7981 gtgtcggctt cgcaggtcag cccttttgca ttcagcgtga cggtatgctg atatcgggga
     8041 ttcagttcac cgctggtgag catgagttcc agttgtttca tcagcagcgg aaaggcctgg
     8101 tccagatggt atgcatctgc gtcgctgaag cggcctctga taccggcgcg gtcggcaagg
     8161 taatgcaggc ggttaccctc ttgcaccaga cgagcgccaa aacagggcct cactgtgcag
     8221 ggaagccccc accaggggcg gtcgtgattg tcatcgggat gagttatccc ggagagtttg
     8281 tctgacacga taaaatcctc tctgaaaagt ggttaagaat ggtttaacga tgacaggtca
     8341 gccggcgcgc tgaccggttt cggtgaagat gtatgcattg atgagaatgg cctcatccac
     8401 agcggcgtct gaggtcatct ggtcgtactc gtttcccggg tgacggaaca tcagttcagg
     8461 gtgacactca tcagccggac atacgggcca aaactgtcct tacggcgttc tgcaaacacg
     8521 gcaagaacgc cgggaatatc ctgcacttca cgaccagtat acgcttcagc actgccgtgc
     8581 cagcgatact taccggtaca gaacggaaaa agacgggatg tcggatgttg ttggtgaata
     8641 cgcatggctt caccacgggt gataattttc ataatgggat acctctgaag acagaagata
     8701 aaagtgaaaa caggtgtgat gtggttgtga agggttaaag cagaccatgt tcggcaaagg
     8761 agaaaacctg gttgccaccg actatcagat ggtccggcac ccggatatcc accaggccca
     8821 gtgcctgtac cagacgttcg gtgataagcc ggtctgcctt actgggtgtg acttcaccgg
     8881 acgggtgatt gtgcgccagc accacggcag cggcattgtg gtacagggcg cgtttaatca
     8941 cttcccgggg atgaacttcc gtacggttga tggtgccggt gaagagggtt tcaccggcaa
     9001 tcagctgatt ctggttgttc agatacagca cccggaactc ttcccgctcc agtcccgcca
     9061 tgttcagaat cagccattcc cgtgccgcac gggtggaggt gaaggccacg ccgggttcat
     9121 gaagatggcg gtccagggtt tgcagggccc gcagaatgag actgcgctcg ccgggcgtca
     9181 tctctccggg cagaaaggaa agctgctgca ttgtccctgc ctccattcag tcaatgatgc
     9241 gcataatggc gctgcattcc ggatgctgca gggcgtaatc ccgcagccgg taatagtgga
     9301 ccgtcatggc ataacactcc gtacgacagg catgatgact gtacgtcatc agacaggcag
     9361 caataccggc ggcttccggg ctcatttcag cgcggttgcc gttcatggca ttgaacagta
     9421 cccatgtttc gtcatcatcg tcatccggtt cgggtgccat aaatgccccg ccgttgttca
     9481 gggtgtacag attccagata ccaccgcagt agtcttcgca aagacggtcc atccagccga
     9541 agacacgggg ctccagggtc acccactgtg gaatgaggcc aaaatgctgc ggccagaagc
     9601 tgatgcgctg ttcatcaggg acgggagtgg caaccagctg aggtgggtta ttccctgatg
     9661 cagtggttac ggaaacagaa ggtgtggtgg aattatgcga aacggttgtc atgaggttat
     9721 tccttataaa aagtaaatga atggaagaag ccccggggca ggaacagatg tgagtcagga
     9781 ctgcactttc agtgaaacgg catcagcgca tgctctccag cagcgtttct gccatcaccc
     9841 acaatgcgcg attgagctta atgtcggtat caatgctgtg aatggcccgg gtatggatac
     9901 gttttccttt tgcactgcga ccggaaatcc cgcctttcag catattctcc tggatggtct
     9961 gataagcact ccacaggtcc ttaccgtaat cctcccggcg tcgtggtgtc agaatgtcgg
    10021 cggtggtgac gggctgatgt tcgtcaccat aacggtaagt cagtgccgcc tgtgccagcg
    10081 cctggcgtgc cggtggcggc aggaccagcg actgcatggc atcacgcttc tcctctatcc
    10141 ggtcaaaaac gcccaccacc tcgtaagccc cttcaatgac tctgtccacc acatttcccc
    10201 ggtgtggaac acgcaattct cccagagact gaccgcagac cagtccattg gtacaaatgg
    10261 cacgaaaata tccgggtaac atctggtagc tggatgaacc gtcatgggag ttgagcagaa
    10321 taatttcagg gacatgctga ccggttatct gtccggcccg ccgcagacgc agcatgtgtt
    10381 ttgtgtattc ccggcggctc tggtcgcgca cgcgggtctg gcaggcaaag aacggctgaa
    10441 agccttcctg ctgcagattt tccaggacgg tgatagtggg aatgtacgca tagtgttcac
    10501 tgcgagaggt atgtcggttt tccccaaaaa tactcggtac atggcgcatc agttcttcgt
    10561 gtgtcagcgg acgatcacga cgtatctggt tcgcataacc aaaacgactg gctaatctca
    10621 tgatttgctc cttatgaatg attaagcgat ataaatgaaa aagccgcgtc tcccggagaa
    10681 gacgcggcct gatgggtgaa atgaatgagg tttattgtct gagaagccct tcactggcga
    10741 accacgtatt aagctgtatt ccggcatcgc cagtgcaact gaccttcagc attgcggata
    10801 acccgccgga aatatgttca ctggttgttt ttgtatcttc ccccaaaatt aaataaattc
    10861 atcttaacca tcacccagat aatatatatc tgaaaatatt ttcaataaca ccagacagga
    10921 aaaacaccac cacaggcggt ggcgtttctt tggggcgtct ttatttaatc cgggccatcg
    10981 cgtttctcag tgccaaatct gcctcttccg ttatatccag gcttttctgc aataccgcct
    11041 ctgaccgggt cagtgcctga cgtgctgcca gcggtttccg gatatgctcc atcgccagca
    11101 tcgtgtcttc atagccctgc tgacgccagg cgtcggtatg tgccgatgta aaactgagca
    11161 gaccgccgct gttgccacca tcacctgcat atttcagcct tttccgggga cggatctcaa
    11221 ggattgtggt gtccggaaaa gcctggcgat cccataaaga accgtcactc agatgcgtca
    11281 caatcaccat attgcatccg gcatcgacca gaggcgtcac aggggtattt ccctgcatat
    11341 ttcgccatcc tcccatacca ccatcaccga acattgtccc ctgaacctca cggggacgga
    11401 acagcagggg cagcgcggct gacgcaagca gagcctcttt ctgctgtccg cggggcaggc
    11461 tctggatatg ctgaaaaacg gcgtttttcg tggttccgac acccagttca gcacgaatgc
    11521 agtcaataat atcctgcatg ccccttctgt ggggtacagc gacacataca gcggtagccc
    11581 gtctgccaga gcatcagtat caagataatg atccatcagc gctgtcaggg gctcatctga
    11641 caacaacggc tgagccatca gattgtcaaa cccgttgatt gtcgatatat ggcgaagaag
    11701 cgtcgtaagc agtgctcctg cacgtccggg gatctggcag aggttcattg cctggaacaa
    11761 ttttttcagc aatgaaaagt aaaccaatct gtttaccgac agcacctgat tattccccag
    11821 atgctcccag agcgcctcca ggcgtacggc agcttctgac agatcgggag aggccgcgat
    11881 aatggcacca ttgaatgcgc caatgctggt ccctgaaacc atgctgatct gtgtaccaca
    11941 ctctgccagt gccttaacca ctcccgcctg ataagcgccc accgccccgc caccggacag
    12001 caccagcccc gttttcattt ctgtactcat gcagccccct tctgaaatat ttcatcgacc
    12061 atactttcag agaggcgttc tgcagagcag aggcgaggca tgcgaaccgg taagtgttct
    12121 gcagcctgcg tggttgacca gatcaccgga tgctgctctt cactgcacca gtctcccagc
    12181 cagttaatcg cgtcatgcca ctcgttatct gcctgctccc ggccctgacc accaagccag
    12241 tccagagtgt tcagacgcac ggttgcacta ccgccactct cctgtgccat ctgcgttaat
    12301 gctttatgtc tgagaatatc cagtagctgg ttaacctggc ctgtttcgcc ctgacgtatc
    12361 acatcaccaa acgcgcccca gcgaagctct tcaagtgcca cataacaacg tccggcaggg
    12421 gaaaatgacg catatcgccc ggcgctccac catgagaata tctgctcaag atgtaactgc
    12481 gcccgctgaa cctggtcaac acggtggagt ttttcttcaa gatgattcag tttatggtga
    12541 acctgatcgg ccagggtgag taactgctcc cgcgtatctg cggaataatg cgccagcctg
    12601 gctgtatcac tgaccagtga actgaccctg tcgtttaccc gggtaatcgc gtagtttgtg
    12661 gtggcaagtg acgttgtcat ctccgtcagc cagcgcaggg acgcctcaac gccctgtgca
    12721 agcgacgcat tgatggcatt ctgccgtgca gcgccttcgc cggtcagccc ctcttttaaa
    12781 cgccggaaga acgtgcgctc tccggccgtg cggatatgct cctgtgcaac atcgattccg
    12841 tttgcgaaat caaccacaaa tttttcagga agacaggtga gtacctgaca tacccggtta
    12901 tcagcaattt gttgaataaa tggtgaagtc acagcattgt ctccagctct tccctcagcg
    12961 ccttactgtc agtaatgagc tcaggaaccc tttcatttaa tgcctgaagt cgcccggtaa
    13021 gccgttcctg cgcctgctga tcgcgtttat gatcttcaga gctctgaatc agcgtgttac
    13081 gcagttgttc aactttctcc ctgaatgcgc agaaaaaggc atcgatttcc tggcggacgg
    13141 gctggttaat atcctgctca atggatgcct gcagctcccc gaaatatgcc cgggtcagtg
    13201 acataacttc ctctctggcc ttgttgatat tgatcacgct gcgactcaca tcctcgtcat
    13261 agttttccca gcccaggtca ctggtgccaa accatcgaca aacggttccc catacgccac
    13321 tctgccgacg gcgacgggtg acggtctccg ttctttcctc cataagcgca ctcagattta
    13381 tttttaccgc gagccgggtc tgtaactggc tgactgccgg aaaactgatt tttacggtaa
    13441 agccctcatc ctccaggcgg gcattgatgc gaccggcaat gttttctgtg gcgtacaggg
    13501 ccgtgccacg aaaacctgtt tcaatcccgc tgacgatttt ctccagctca ggccggaact
    13561 gtgcttcagc ctcacggtgc aggctggtca cggtcgattc gatttgcgtc atcagttcaa
    13621 gggctgccct gcgatcactg aattttattt cggggctgtc cggatcaaag tcccgcatcc
    13681 tgtcatgctg atttccggtt ccgaaaatat cgtgaaagaa agcgaatgcg tttctctccc
    13741 ggggctgcgc tgaattttct tcctccagca tttgctgttc tttgcgttta ccgcttctga
    13801 aatagtcgtt cagctcctcc tccagtgagg cacagacctt cgtcagtaat tccccggttt
    13861 tggtattgat gtcctccatg gccattctga catcaccggt cacctgattc tgacattcct
    13921 caatctcctg gatatccgcc agcaggctgg taatatgcgc ctgtagtgac tgaatgcttt
    13981 gctgcagccc ctgatgacgt aatgacagat attcgctgac attttctgca ttctgcatca
    14041 gttttgcggc ggcagagtca accgccagtg ccgccgctct ggaatgggag ctctgtatga
    14101 cttccgttat cagttgatcg atcagagata tattccagag tttttctgcc tttttattta
    14161 ccttagtgct gtctttccag tcgtcctctt ccaccatagg tccgaaagct tccctgataa
    14221 aatcatcgac ccaggcttca tcaaccggaa gcgcaccgtt ttttcgtaac tcatgaagcg
    14281 cacggttagc caggtacgcc tgacgtgatg agccgggata aacgcgggag gcgggcagca
    14341 catcgctgtt cagcattgct ggcaccttct ggcgtacggc atccgcacca tcaccattgc
    14401 gatctttttc atcgaattta ttgaccagca caaacagacg accggctgag acgtcagcaa
    14461 tggcattaag ctctttacga acctcctcat cggctttcga gttcatctgg gtgtaatcca
    14521 tcaccgccag caccgcagag gctttctgta gttgatcccg catcatcatt tccatctgtg
    14581 gttgtccggc ttcattaggg ccgggggtat ccagcagtgt gagtgttccc tgacatgcat
    14641 ccattcccac caggtggctg aacgccacgt cgatcaccgg tagtttctgc acttctgcgt
    14701 attcgtcaaa aggaaattca gtccccattg ccgcagcaag acgaaccagg tcatttaacg
    14761 atgccagtcc ggtaaagatt tcgtcttccc cgtggtattc atttttaagc cagacatcat
    14821 ccgccagaat atccagtagt tcgcgggtat ccccggtttg ctgcagggac tgtgcgactt
    14881 gctgccctgc cggggtggcg agtttttcct gcagtgtgat taataaattg cggacgggct
    14941 gaatatgttc cagatggagg accggttccg tttttccggg aacgtggcgg atcaatgtcg
    15001 gtaccgaggt catggggcgg ttacggttgg gcagaatttc ctgccccaca atggcgttga
    15061 tggtggttga tttgcccgcc ttcatcgtgc cgaccacagc cagcaccatc tccctgcggg
    15121 cggttttgat ttgttcgccc tccagctcct caattctttt cagcgctttc cgtttatcaa
    15181 aaagcagccc gttttcgttc ttactgtcag ataacacatc cggctcatca accatttgcc
    15241 ggagcagatt gatattcagt tgcaaaagcc ggtcggcttc atcacaaagc agggcgatgt
    15301 tcttttcgtg catcattcac ctttataaat tcaataattc agaactgcgg tgaacagcaa
    15361 tgagcgtcct ttcccattac tgcatgaatt ttcctgaaat cgtatttccg ggataccgta
    15421 taatcagaaa gtcatattca gcgtagcctg accgttatag ccttcagcgc tgctgccgct
    15481 gacgctgtgg gcataaccgg cctgaacgcc cagggtgata ttttcccgga tacgggcttc
    15541 cagtccggcc tgcaggtcca gtgacgtgcc attccgggac ggtgagaacg tcatgttact
    15601 gccggctgcg gctgtcccca tgctcatgtc accccgggag ctgaaggtgc ggataacaga
    15661 aggctgtacc caccagttca ccggcagttc actcacactg tgttttgcac tgtcgcgcag
    15721 ggtgtcacgg gatgaggtgc cttcaccaaa ggtcatatcg ttgtggctgc ccagacggaa
    15781 accggcacgc acatgttgtg cactgccatg cccgaacttc acataaccgg cgttatcctg
    15841 gccgtcatcc agggagagtc cctgccaggt gtactgcagt tgtggctcca gcatcagatt
    15901 gtcagtgata ctgaagggca gaccggtttc cagtgagccc agccagcccc agccccgggc
    15961 gcggaagtcg ttattgtccg atgacgcttt catgctgtga cgggttccct gggccacaat
    16021 gtcagcccac aggccggagg atgtgtgtac cagattcagg tatccgccca gactgccggc
    16081 atcatcccgg accgtgccgg cgcgggaacc gtcatcatcc ttaacatcaa cggaagaatg
    16141 gcctgcagca ccatacaccc ctgtcgtcag agacataccg gcaacctctg ttctgagcag
    16201 gtcaccctcc agacggacga agccatagct gccgctgctt tccggcgtgg ctccacgggc
    16261 aataccgccg ttgttatcgt gaccgagatg accgccctga atgctgagac ggacgctgtt
    16321 attttcaccg tttacaccgg tctgatggct gcgggagcct gccagaatcc ggtcatagtc
    16381 cattgcctgt gtcaacatgg atgtatacag ggggacttca gcacgataag cattttcact
    16441 gcgcaggtac cagtcttcat cgctgtcacg gttcagggtg tagttaaagg cgccggcctg
    16501 aagcgggcga ctcagggcaa acgcaccttc ttctgtggtg gcgccattct gtgcatccac
    16561 aacccggata ccctgtccgg tggttgccac cccgaggttg ctgtttccga catttgtaaa
    16621 cgcaagccag gttttgccgg ttgcctgacc accattaatc accagctggt cagaggcatt
    16681 gctgccatca aggcgaacac gcatattgat ggtgccgccc tggccggtga ggttcgtggt
    16741 ggtcagttta tggaacgtta ccggggagtt actttttgca acagcacggc tcagcgcggc
    16801 attcggtgtc gtctgattat caaagcgaat ctcccctgcg ttaacaatat cgccggtact
    16861 gaccacatca ccattcagta ccatactggc gcctttactg agctgtgttc tgccactcag
    16921 acttcccccg gcagccagcg tcagtgttcc acgatgtccg acagtggtgt tgccagcctg
    16981 tcccccggca ttaaccgtaa agctgccgcc attttccagc agcaggccgc tggcctgacc
    17041 ggatgtgcca tcaatactga acttaccgct ggcattggtc ccctcaacag tggcaccact
    17101 gtctgcaatc agggcaccac cggatgttat ggtgacactt gtcgccttac cgccggcaga
    17161 cactgccagg gtaccgccgt catccactag ggtattctgt gctgaatggc tctccagtac
    17221 atccagacga ccgccggatt ccagaacaac accgtcagcc ttaccgtttt ccaccgtgaa
    17281 attccccaga cggtttgtgc cgatgacagt tgcagcagta ctggtaacca gtgcaccgcc
    17341 cgtgttctgg gtgactgcag tggcttcccc gccggcatgt accctcagtt caccgttctg
    17401 atttatggtg gtgttaccgg cagcgccacc tgccttaaca acctgccagc ccccctcgtt
    17461 aattaccgtg ttcagcgcaa gtccgtccct gtgcacatac tggtaaccgc cattcagtgt
    17521 ggtattcagg gcccttccgt gtaccgactg gtcaccacct gcgtaaatga gagtgtcacg
    17581 ggctagcccg gaaaataaga taatctgccg tccatttttg ttgatggtgg tggattctgc
    17641 tgttccctgg accttctgac ccgtatacga gttgtcagaa tcagggccgc cttctgcgcc
    17701 ggtgttgatg atggttcctg ttgccagccc gccactttta acgctctggt aaccgtcgcg
    17761 gttgataatt gtaccggtgg caaccccgcc ctcatgcacc cactgctcgc ctctgttatt
    17821 cagtgtggtg ttcaccgcca gtccgttcag gctctgtccc ccgccgtcac gaataaccgt
    17881 atcactggct gttcccccct ccagcacgac ctgacgacca tttgtggtga cagtggtgtt
    17941 tccggctatc ccgccattct gtatccattg cccaccggtg ttttcttcac tgtccggccc
    18001 cagttccagc ccggtactga tggtcattcc gttggccgta ccgaagacaa tctggttgtc
    18061 atgatttgtc agtgttccat cgttcacggt ttctcccgcc tgtacaacct tgtcagcagc
    18121 cagtgccggg actgatgtga cagcagcaag agacagcgca accgccacac cggcgcgttt
    18181 tccccgtgag cgggccagtt cggaggccac caccagggtg cccgtaatgt gattccatac
    18241 cagcctgtag ctggtgttca gatgtcgttt catcagcttt tccttacaga gggtgaataa
    18301 aaaagccggt acccacaaaa gtggataccg gcaggtacaa aacagagtta tcgggcacag
    18361 cgatacgtcc cgggcgtaaa cgctctcccc cttcatcaga cagagaagta tcagtctgtt
    18421 gtgtgcaatt ccgttctgca tgatgaagag attcagctgc cttttcgcag gccattaacc
    18481 actataaacg atcgatataa acgatcgata cctgttttat cgatcgtttt attctattaa
    18541 cagtagagca gtcacgcaag agaaacagga ggggcatgag gtgtttgtat catgttttca
    18601 gttggttaac gcaatgtaat cagacatttc ccctggcaga ctgttcagaa gaaaatccag
    18661 ttccataccg cgcgggcaac agagaccacc gtgtcacgga cggcacgcag aactgtgcgt
    18721 gcaacagagg caacacagac gctctccacc gtgtcaaata tccggtccac cgcaccggta
    18781 aactgttcac gggcctgccc ccggacagat tccgtgcaca gctcgtcctg caggcgggtc
    18841 atcaggggac tggcggcatg gtcgggaagc gctgtcatga gcgcactgac cagcgtatcc
    18901 agttcccagc cggtgcgggc cgatacggca acaaccggat gtacgggccg gaacagccgg
    18961 aataccgcat ctgttttttc gcgaatgttc tgttcctgtg cgggagaagg ctgaatacct
    19021 gccatatccc attcatggca gggctctgtt ttgtcggcct gcgtcaccac aaacagcacc
    19081 tgctgatgtc cccggtgcag tatgtgtcgc cagaaatact catccacaga cagggcacgg
    19141 tcatcggctt taatcagcca cagtaccaag tccagttcag gcagaatgtc acggtacagg
    19201 gcttcatact ctgcatccct gtcccggctc tcgcccaccc cgggcaggtc agtgataacc
    19261 atgctgtgac catggccact cagacggaag cgccgcactt cccgggtgcc ggcgtgaaca
    19321 tcactgaccg gggtgacc
//
LOCUS       DAZFWL020000085        18973 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFWL020000085 DAZFWL020000000
VERSION     DAZFWL020000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.085"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            144..710
                     /gene="yqaB"
                     /locus_tag="ACHKNP_RS15990"
                     /old_locus_tag="pgaptmp_003198"
     CDS             144..710
                     /gene="yqaB"
                     /locus_tag="ACHKNP_RS15990"
                     /old_locus_tag="pgaptmp_003198"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HGU4705315.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKYHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            707..1135
                     /locus_tag="ACHKNP_RS15995"
                     /old_locus_tag="pgaptmp_003199"
     CDS             707..1135
                     /locus_tag="ACHKNP_RS15995"
                     /old_locus_tag="pgaptmp_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HGU4705316.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1208..2764
                     /gene="gshA"
                     /locus_tag="ACHKNP_RS16000"
                     /old_locus_tag="pgaptmp_003200"
     CDS             1208..2764
                     /gene="gshA"
                     /locus_tag="ACHKNP_RS16000"
                     /old_locus_tag="pgaptmp_003200"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HGU4705317.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            2914..3429
                     /gene="luxS"
                     /locus_tag="ACHKNP_RS16005"
                     /old_locus_tag="pgaptmp_003201"
     CDS             2914..3429
                     /gene="luxS"
                     /locus_tag="ACHKNP_RS16005"
                     /old_locus_tag="pgaptmp_003201"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGU4705318.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3493..5031)
                     /gene="emrB"
                     /locus_tag="ACHKNP_RS16010"
                     /old_locus_tag="pgaptmp_003202"
     CDS             complement(3493..5031)
                     /gene="emrB"
                     /locus_tag="ACHKNP_RS16010"
                     /old_locus_tag="pgaptmp_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HGU4705319.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5048..6220)
                     /gene="emrA"
                     /locus_tag="ACHKNP_RS16015"
                     /old_locus_tag="pgaptmp_003203"
     CDS             complement(5048..6220)
                     /gene="emrA"
                     /locus_tag="ACHKNP_RS16015"
                     /old_locus_tag="pgaptmp_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HGU4705320.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6347..6877)
                     /gene="emrR"
                     /locus_tag="ACHKNP_RS16020"
                     /old_locus_tag="pgaptmp_003204"
     CDS             complement(6347..6877)
                     /gene="emrR"
                     /locus_tag="ACHKNP_RS16020"
                     /old_locus_tag="pgaptmp_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HGU4705321.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(6968..7303)
                     /gene="ygaH"
                     /locus_tag="ACHKNP_RS16025"
                     /old_locus_tag="pgaptmp_003205"
     CDS             complement(6968..7303)
                     /gene="ygaH"
                     /locus_tag="ACHKNP_RS16025"
                     /old_locus_tag="pgaptmp_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HGU4705322.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7293..8030)
                     /gene="ygaZ"
                     /locus_tag="ACHKNP_RS16030"
                     /old_locus_tag="pgaptmp_003206"
     CDS             complement(7293..8030)
                     /gene="ygaZ"
                     /locus_tag="ACHKNP_RS16030"
                     /old_locus_tag="pgaptmp_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HGU4705323.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8154..9338)
                     /locus_tag="ACHKNP_RS16035"
                     /old_locus_tag="pgaptmp_003207"
     CDS             complement(8154..9338)
                     /locus_tag="ACHKNP_RS16035"
                     /old_locus_tag="pgaptmp_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4705324.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     LLGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFINDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9530..10522)
                     /gene="proX"
                     /locus_tag="ACHKNP_RS16040"
                     /old_locus_tag="pgaptmp_003208"
     CDS             complement(9530..10522)
                     /gene="proX"
                     /locus_tag="ACHKNP_RS16040"
                     /old_locus_tag="pgaptmp_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HGU4705325.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10580..11644)
                     /gene="proW"
                     /locus_tag="ACHKNP_RS16045"
                     /old_locus_tag="pgaptmp_003209"
     CDS             complement(10580..11644)
                     /gene="proW"
                     /locus_tag="ACHKNP_RS16045"
                     /old_locus_tag="pgaptmp_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HGU4705326.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11637..12839)
                     /gene="proV"
                     /locus_tag="ACHKNP_RS16050"
                     /old_locus_tag="pgaptmp_003210"
     CDS             complement(11637..12839)
                     /gene="proV"
                     /locus_tag="ACHKNP_RS16050"
                     /old_locus_tag="pgaptmp_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HGU4705327.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13194..14153)
                     /gene="nrdF"
                     /locus_tag="ACHKNP_RS16055"
                     /old_locus_tag="pgaptmp_003211"
     CDS             complement(13194..14153)
                     /gene="nrdF"
                     /locus_tag="ACHKNP_RS16055"
                     /old_locus_tag="pgaptmp_003211"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU4705328.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14163..16307)
                     /gene="nrdE"
                     /locus_tag="ACHKNP_RS16060"
                     /old_locus_tag="pgaptmp_003212"
     CDS             complement(14163..16307)
                     /gene="nrdE"
                     /locus_tag="ACHKNP_RS16060"
                     /old_locus_tag="pgaptmp_003212"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU4705329.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYPNQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16280..16690)
                     /gene="nrdI"
                     /locus_tag="ACHKNP_RS16065"
                     /old_locus_tag="pgaptmp_003213"
     CDS             complement(16280..16690)
                     /gene="nrdI"
                     /locus_tag="ACHKNP_RS16065"
                     /old_locus_tag="pgaptmp_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGU4705330.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(16687..16932)
                     /gene="nrdH"
                     /locus_tag="ACHKNP_RS16070"
                     /old_locus_tag="pgaptmp_003214"
     CDS             complement(16687..16932)
                     /gene="nrdH"
                     /locus_tag="ACHKNP_RS16070"
                     /old_locus_tag="pgaptmp_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGU4705331.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17180..17509)
                     /locus_tag="ACHKNP_RS16075"
                     /old_locus_tag="pgaptmp_003215"
     CDS             complement(17180..17509)
                     /locus_tag="ACHKNP_RS16075"
                     /old_locus_tag="pgaptmp_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HGU4705332.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            17661..18005
                     /locus_tag="ACHKNP_RS16080"
                     /old_locus_tag="pgaptmp_003216"
     CDS             17661..18005
                     /locus_tag="ACHKNP_RS16080"
                     /old_locus_tag="pgaptmp_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HGU4705333.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18042..18491)
                     /gene="alaE"
                     /locus_tag="ACHKNP_RS16085"
                     /old_locus_tag="pgaptmp_003217"
     CDS             complement(18042..18491)
                     /gene="alaE"
                     /locus_tag="ACHKNP_RS16085"
                     /old_locus_tag="pgaptmp_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HGU4705334.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPANILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         4757 a         4784 c         4896 g         4536 t
ORIGIN      
        1 ctcccaaaaa tttccttcaa aaatatctca tttctaaata actactttat ccatttcctg
       61 aattttcttc tccttcgcca tcagcgacaa aatcaagcaa ttacgctaaa gtagcgtcat
      121 atttttcgac ttgcgagaag accatgtacg agcgttatgc aggtttaatt tttgatatgg
      181 atggcacaat cctggatacg gagcctacgc accgtaaagc gtggcgcgaa gtattagggc
      241 actacggtct tcagtacgat gttcaggcaa tgattgcgct taatggatcg cccacctggc
      301 gtattgctca ggcaattatt gagctgaatc aggccgatct cgacccgcat gcgttagcgc
      361 gtgaaaaaac agaagcagta agaagtatgc tgctggatag tgtcgagccg cttcctcttg
      421 ttgaagtggt gaaaagttgg catggtcgtc gcccaatggc tgtaggaacg gggagtgaaa
      481 gcgccatcgc tgaggcattg ctggcgcacc tgggattacg ccgttatttt gacgccgtcg
      541 tcgctgctga tcacgtcaaa taccataaac ccgcgccaga cacatttttg ttgtgcgcgc
      601 agcgtatggg cgtgcaaccg acgcagtgtg tggtctttga agatgccgat ttcggtattc
      661 aggcggcccg tgcagcaggc atggacgccg tggatgttcg cttgctgtga gtgaagcgtt
      721 atcgcttttc tcgttgtttg ccagtagttt tctcagcgct acactattac ccggcaactc
      781 cgaagtcgtg ctggtggcaa tgttgctttc cgggatcagt tatccctggg ttttagtttt
      841 aacagcaaca atgggtaata gccttggagg gttaactaac gttatccttg ggcgtttctt
      901 tccattgcgt aaaacatcgc gctggcaaga gaaagctacc ggctggctga aacgctatgg
      961 tgcagtcacg ctattattaa gctggatgcc cgtggttggc gatttactgt gtctgttagc
     1021 gggatggatg cgcatctcct gggggccagt aatctttttt ttgtgccttg gtaaagcgtt
     1081 acgctatgtt gcagttgcag cagcgaccgt tcagggcatg atgtggtggc actaattgta
     1141 ggcctgcaca tatggtcacc attacagtta tgctaattaa aacgattttg acaggcggga
     1201 ggtcaatttg atcccggacg tatcacaggc gctggcctgg ctggaaaaac atcctcaggc
     1261 gttaaagggg atacagcgtg ggctggagcg cgaaactttg cgtgttaatg ctgatggcac
     1321 actggcaaca acaggtcatc ctgaagcatt aggttccgca ctgacgcaca aatggattac
     1381 taccgatttt gcggaagcat tgctggaatt cattacacca gtggatggtg atattgaaca
     1441 tatgttgacc tttatgcgcg atctgcatcg ttatacggcg cgcaatatgg gcgatgagcg
     1501 gatgtggccg ttaagtatgc catgctacat cgcagaaggt caggacatcg aactggcaca
     1561 gtacggcact tctaacaccg gacgctttaa aacgctgtat cgtgaagggc tgaaaaatcg
     1621 ctacggcgcg ctgatgcaaa ccatttccgg cgtgcactac aatttctctt tgccaatggc
     1681 attctggcaa gcgaagtgcg gtgatatctc gggcgctgat gccaaagaga aaatttctgc
     1741 gggctatttc cgcgttatcc gcaattacta tcgtttcggt tgggtcattc cttatctgtt
     1801 tggtgcatct ccggcgattt gttcttcttt cctgcaagga aaaccaacgt cgctgccgtt
     1861 tgagaaaacc gagtgcggta tgtattacct gccgtatgcg acctctcttc gtttgagcga
     1921 tctcggctat accaataaat cgcaaagcaa tcttggtatt accttcaacg atctttacga
     1981 gtacgtagcg ggccttaaac aggcaatcaa aacgccatcg gaagagtacg cgaagattgg
     2041 tattgagaaa gacggtaaga ggctgcaaat caatagcaac gtgctgcaga ttgaaaacga
     2101 actgtacgcg ccgattcgtc caaaacgcgt tacccgcagc ggcgagtcac cttctgatgc
     2161 gctgttacgt ggcggcattg aatatattga agtgcgttcg ctggacatca acccgttctc
     2221 gccgattggt gtagatgaac agcaggtgcg attcctcgac ctgtttatgg tctggtgtgc
     2281 gctggctgat gcaccggaaa tgagcagtag cgaacttgcc tgtacgcgcg ttaactggaa
     2341 ccgggtgatc cttgaaggtc gcaaaccggg cctgacgctg ggtatcggct gcgaaaccgc
     2401 gcagttcccg ttactgcagg tgggtaaaga tctgttccgc gatctgaaac gcgtcgcgca
     2461 aacgctggat agcattaacg gcggcgaagc gtatcagaaa gtgtgtgatg aactggttgc
     2521 ctgcttcgat aatcccgatc tgactttctc tgcccgtatc ttaaggtcta tgattgatac
     2581 tggtattggc ggaacaggca aagcgtttgc agaagcctac cgtaatctgc tgcgtgaaga
     2641 gccgctggaa attctgcgcg aagaggattt tgtagccgag cgcgaggcat ctgaacgccg
     2701 tcagcaggaa atggaaaccg ctgataccga accgtttgcg gtgtggctgg aaaaacacgc
     2761 ctgacagaaa agaaaaaggc cactcgtgag tggccaaaat ttcatctctg aattcaggga
     2821 tgatgataac aaatgcgcgt ctttcatata ctcagactcg cctgggaaga aagagttcag
     2881 aaaattttaa aaaaaattac cggaggtggc taaatgccgt tgttagatag cttcacagtc
     2941 gatcataccc ggatggaagc gcctgcagtt cgggtggcga aaacaatgaa caccccgcat
     3001 ggcgacgcaa tcaccgtgtt cgatctgcgc ttctgcgtgc cgaacaaaga agtgatgcca
     3061 gaaagaggga tccataccct ggagcacctg tttgctggtt ttatgcgtaa ccatcttaac
     3121 ggtaagggtg tagagattat cgatatctcg ccaatgggct gccgcaccgg tttttatatg
     3181 agtctgattg gtacgccaga tgagcagcgt gttgctgatg cctggaaagc ggcaatggaa
     3241 gacgtgctga aagtgcagga tcagaatcag atcccggaac tgaacgtcta ccagtgtggc
     3301 acttaccaga tgcactcgtt gcaggaagcg caggatattg cgcgtagcat tctggaacgt
     3361 gacgtacgca tcaacagcaa cgaagaactg gcactgccga aagagaagtt gcaggaactg
     3421 cacatctagt cagtaaacta tcttcacaat taattgaaaa aagccagttc aaatgaactg
     3481 gcttagttgt acttagtgcg caccgcctcc gccgccacct gcgccaaatg gcggtttagc
     3541 aaaccacacc agccccagca gtacgaggaa tattccggct gacatccaga agatctcatt
     3601 ggcggaaata atcagcccct gattggtgat ctgctgggca atccagcctg atgcctgctg
     3661 ttgcgtcatc ccaagccctt ccagttgact gtacatcgcc tgggcattcg ggttgaacgg
     3721 gtttaccgac tcagtcaact gcgcatggtg catcgactcg cggttggtcc acatggtcgt
     3781 ggttatcgac gtgccgatag accccgccag cgttcgcgta aagttagaga ggctcgatgc
     3841 cgccgccagt cgttccggtg gcaaaccaga cagcgtaatg gtggtcagcg gcataaagaa
     3901 gcaggccacc gcaaacccct ggataaactg cggccaggcc gacgcgccaa aatccatacc
     3961 tggttcaaag gtataggcac gccagtagaa gcagacggca tacataataa agctgaaggt
     4021 taccagccgc cgcatatcca gtttatgcgc gaagcggccg ataatcggcg acaggatcac
     4081 cggaataatc cctaccggcg cagaggccaa acctgcccag gtcgccgtgt aaccgtagac
     4141 ctcctgcaac aactgcggca gcagaacaat agcgccgaag tagagcatat acgcgaggct
     4201 gatacacaag cagccgatgg tgaagttgcg cgacttaaac aacgacagat cgactatcgg
     4261 gttatcgtcg gtcagctccc agacaatcag gaagcagata gccaccaccg ccaccacggt
     4321 aaggatgata atttcctgtg atgaaaacca atccagctct ttaccgcggt caagcataat
     4381 ctgcaggctg ccgataccaa taaccagcag tgccagcccc acggcatcaa tccgccgccg
     4441 ttcggtgcgg gtttcacgtc cgcgcagagt ttgcagtgtc atcaacacca ccgccacgcc
     4501 aatcggcacg ttgatgaaga atatccagcc ccagtggtaa ttatcgctga tataaccgcc
     4561 gaggatcggg ccgcaaattg gcgcgacaat caccgtcatc gaccacaacg ccagcgcgat
     4621 cgagcgtttg gctggcgggt agttattcag caatagactt tgcgaaagcg ggatcaacgg
     4681 cccggcgaca atcccctgaa tcacgcggaa gaagatcagc atattcaggc tgctggagac
     4741 accacacgcc cacgacgcaa tagcaaaggc gatggtggac caaaggaaca gtttcacttc
     4801 cccgacgcgc tttgccagcc agccggtaag cgggatcgag atggcattcg ccaccccgaa
     4861 agaagtgatt acccacgttc cctggctgag cgatgagccc agattcccgg cgatagtggg
     4921 gatcgccacg ttagcaatgg tggagtccag cacctgcatg aatgtcgcca gtgacagcgc
     4981 aatcgtcata atgaccagtt gcgcgccttc cagcggtttt tgctgttgca tcacacgcac
     5041 ctctggatta gccagcgtta gcttttacga tatcgtcgat cagtttattg acaggtgcca
     5101 ggctgatttc acgcgcggta cttaccgcga ccggtgttga acgtacttta tttgccagta
     5161 cctgaccgtc acggttagtg gtattgacgc tcaccagcgt ggacaaacca atgcgcagcg
     5221 ggtattgctc cagctgtttc tggtccagtt cgatacgcac aggtagacgc tgaacgactt
     5281 tgatccagtt accggtcgca ttttgcgccg gaagcagtga gaatgcgcta cctgtgccca
     5341 tatccagacc aaccacttta ccggtgtatt tcacatcatc gccgtaaata tccgtggtga
     5401 tagtgaccgg ctgaccgata cgcatattgg caatctgcgt ctctttaaag ttggcatcca
     5461 cccacatatt ggtggctgga acgaccgcca tcagcggcgt cgttgggcta atttgcgccc
     5521 caggctgtac cgcgcggcgg gagacataac cggtcatcgg actgacaata cgggtacgct
     5581 ccagcgccag ccaggcgtta cgtacttcgg tggcagcctg ttgcacggca ggctgatctt
     5641 ccagtttagt ccccagaatc atcgcctgat tggcattgta ttgttgaata gcgacgtcca
     5701 gttgcgcctg ggcactggtg acggcgtcgc gggcgtgttg cagctcttcg cgaccaatca
     5761 ggttggcatt gcccagcggc acacggcggt tgtagtcgct ttgtgctttc gcgagggcga
     5821 ttttttgcac ctcaatattc gcctgcaact gtttgctgtt aatcatcagc tggtgggttt
     5881 ggcgaacgct ggaagccagt gcagttttgg ctttttcaaa cgcctggcga gcatctgtcg
     5941 ggtcgagagt gaccagaaca tcgccttctt ttacaaaatc ggtgttatcg gcccagactt
     6001 tcgtcacgct gccagacacc tgagacataa tttgaatctg attccctgcc acgtatgcgt
     6061 catcggtttc ttcgaaatga cgcagtacca aaaaccaata aatccctatc gctacggcaa
     6121 taattataaa gagcaaggtg agaaggagga gcagacgctt acgtttgccg ctcttcttta
     6181 ccggttgctg cggggtttga gtctccgcat ttgcgctcat attgttctcc acgatcttct
     6241 taactcatcg gctgagccga gctgcttgcc aaaaaggcca gcatgttgcg atgctggccg
     6301 gtcatttttt cttttataaa tctggatttt tgagcgagat gacgcgttag ctcatcgctt
     6361 cgagaaccac accgtcttgt tccatctggt cgagacggga gagcaatttg cgggtgattt
     6421 gctcgagctg atctttttct gttgtgctga gcgcggacca gagttgatgc aggcagttat
     6481 gctgcggtgg taaaacctcg cgcaaaaact cgtgaccttt ttccgttaat tgcagatgca
     6541 ggcagcggcg atcgttatcg ctttcacgac gttcgatcca accgcgtttt tccagttcat
     6601 cggcaatacg cgtcgcgttg gtacgggatg atccaagagc acaacttaat tcagaaggct
     6661 gaatactgtg gttttcctga gactccagcg taatcaacgc cataaacaac gtctcgttaa
     6721 tcccctgagc cttcagcatt ttattgcggt tctccagcag cttgctttgc atgtgcatgc
     6781 aaagacgagt cagaaggatc tcctgataag gaaaatcttc gtggcggctg gcgcgaaatt
     6841 ttagcatttg ttcaatgggc gtaaacgaac tatccatttg ggtatgacct cattaatttc
     6901 agccgatata gtaacgacag tgacaaataa agtaaatgta ttgttttaga aaaatgattc
     6961 ttgtgggtta tataatcgcc atcactttcc aggcgagccc ataggccagc gcactaagca
     7021 gtgttgggat gataatgctg cgtgttttat agaaactggc acccagtacc gcgaagccga
     7081 ccagcgtggg cacgaaacgg cgtgtatcgt gcatcacttc tggtgcggta gagacaacca
     7141 gcagagcgca tatcgaggcg atgccaatgg tgtcgagcaa aatacctacc gcgccacgtt
     7201 tggttgggcg ggcattaccc acacgcaggc gcagcggcaa atagcggaag caataattcg
     7261 ccacgccaac cagtaaccca agcagcagaa cctcatagct catcgggcgc tccttgccag
     7321 aatgcctgga ttaacgcagt gaggcagcca cagacaatgc ctgccagaat ggcgacggga
     7381 atagaaaata gcgttacgcc tgcaagggca ccaactaacg ctgcggtaac gcaaagagat
     7441 tgtttgcgct ggaaagaggc gagcaggaaa ctcataaaga gtgccggaag cataaaacct
     7501 aatgcagctt caacggcggg ataaccttgc agcaagccgc tgccggagaa tgcccctatt
     7561 accgtaccaa atacccacga tgaccatgaa ctgaaggcaa tgccgatcat ccagttctcg
     7621 ctccagcggc gattattgcg taccagtttt gcggttgcgg cggcaaaaac ctcatccgtc
     7681 aggccaaatg cccacagggc ggttttcgat ttttgcagac gctgaataat acggctacgc
     7741 agtgacgggc catacaacac atggcgaaca tccattgcca tgacggtcag tgcagcaacc
     7801 cacaaactac tcccggctgc cagcatcgcg gtaatgacga actggctcgc gcctgcataa
     7861 atgatgcagg agaaaaaaac gctttcgaga ggagagaatc ccagacgggt cgcattcaga
     7921 ccgaacgcaa aggccaccgg aatataacta ataacaatcg gtaaactgtc tttgcatcct
     7981 tccatgaagg tcgccgaacc aggagcaggc tgtggagtag ggctttccat agagtgtacg
     8041 cttaacgatt gttaatatta taaatagact gaatgaatat cttaacctta tcagactgat
     8101 gggcttctta acacccttat aagtgtaaag ccacgaaaac ggttgctgat tgcttaattt
     8161 gcggcttcct gacgatgaaa acctcgccac cagaccagta agttaaccag ggcaatcgtc
     8221 gcgccagcca gacaaacgcc agcccaaccg ccatgttgcc aggctgaggc tgaaattagc
     8281 gaaccggcgg caccgccaat aaagtagctg gtcatgtaac ctgcggtcag gcgattacgc
     8341 gcatcagggt gtattcgata aattaccgtc tggttagtga tatgcacgcc ctgtacggtg
     8401 agatccagca ccaggattcc gataatcaac gccagtacgg aagtgtgacc aaaccagatc
     8461 gccagccatg aaagtaatag cagcagcaga ccgaaagttg tggtgtggtg cgatttaccc
     8521 ttatcggcaa aaccgcccgc cggacgagcg cccaacgcgc cggcagctcc cgcaagtcca
     8581 aacagaccaa tgacaccatc gctgtagtta aaaggtggag cggcaagcaa aaaggccatt
     8641 gaggtccaga gaatgctgaa gttggcaaag gtcaggcagc caagcaatgc gcgggtgcgc
     8701 aggatcttat cgttgataaa catgctgaaa acggaaccca acaactgtgg gtagttgagg
     8761 tgggtttctg atttcatttg tggcagacca cgccataatg ctagcgccat cagagccatt
     8821 aacaccgagg caacccaaaa gacggtgcgc cagccgccga ggtttgccag taacccggca
     8881 acggttcgtg ccagcaatat ccccaacagc agaccgctca taatggtgcc aaccactttg
     8941 ccgcgtttat ccggtgaagc cagcgtcgct gccagcggaa ccagaatttg tgccacgaca
     9001 gagaataaac cggttaatgc cgtaccgagg agcatcatcg ccagcgactg actgctggcg
     9061 gtaatcaaca taccgcccgc tgccagtaag gtcatcgaga caatcaggcg gcggcgttca
     9121 aacatatcac cgaggggaac aagaaacagc agacctgctg catagcccaa ttgcgcggcg
     9181 gtaacaataa agcctgccga actggcggaa agggaaaagt tacgcgcgat ggtgtcgagt
     9241 agcggctggg cgtaatagtt actggcgacc gccagaccgg tggcgataga catcagcacg
     9301 atcagcgccg ggctaagctc atgattaggt ttagtcatta tttttgttca tcaggtgatg
     9361 attaagctgg aaatcaataa tagcggatgt tgatgaatat gggatgaaag atagcttgtt
     9421 gaaatgtgcg atgtggtttg taggcatgat aagacgcgtc agcgtcgcat caggcattgt
     9481 gtacagagcg gcggatgagg caaaaacgcc ttatccgccc gaataaaaat tacttctgcg
     9541 ctgccagcgc ctcattcacc cagccatcga actgctgctg gtgggctttg atccagccat
     9601 caacatggcc ctgaatatcg ccttctgagg ctttgccgtc atgcataatg gcgttctggg
     9661 cgttaatatc tgccactggc aactgcataa tggcaaacag tttcgctgct gccgggtttt
     9721 tctcggccca ggctttgttg gcaacgatat gcatggtgct gaccgggaag ccataattcg
     9781 caccattcgg cagtttggta tcggcgtttt tatcgcccgg cagtgcggag aacggcacct
     9841 gcaaccagac cacatctttc cctggcttca gctcattact cacccagtac ggcgtccagg
     9901 tgtagtaaaa caccggtttg ccctctttgt agcgactgat ggtgtcggcc atcatcgccg
     9961 cgtagttccc ctgattatgc gtcacggtat gggtcagttc atacgcggca agctggtggt
    10021 tgatcgcacc ttcgcagccc cagccagggt tacaaccggt taaatccgct tttccgtcgc
    10081 cgttggtatc gaacagtttg gcgatcttcg gatctttcag ttgtgcgatg ttggtgattt
    10141 tgtactggtc ggcggttttc ttatcgatca ggtaaccctg tgccgcgccg ttaacaaata
    10201 ccccttcacg ataaaatttc ttatcgccac cggcagcttc gtacatgttg tcatgcagtg
    10261 gcgtccagtt cacggcggtg aaggttgcat cgccggaagc aagcgaggtg tagccaacgt
    10321 tgtaatccac ttcgctgggt ttgttgacgg tataacctaa tttctccagc gcacgactga
    10381 ccagcagcgt ctggaaggtt tcttcagtga tggtgctctg aactggatta acagtaatgc
    10441 ctttgcccgg cagatcggca gcaaaagttt gtgtagagat aagcgtggca aacgctgtcg
    10501 caaaaagtac gctatgtcgc attgttattc ctttttttgg cagggtgata atgcccggcg
    10561 tcaccgggca agtgcagagt tacttaatga atgggcgggt cagcagacca acagggccag
    10621 tggtgtacca gcgacggttg ccgcgactgc gtgagtcgcg cccaacggcc tgcgtcaggc
    10681 gatcgaggat aatggcgagg atcacaatcc cgacgccgcc aacggtggca agccccatat
    10741 ccagacgacc gataccgcga agtaccatct gacccagccc gccgacggca atcatcgagg
    10801 caatgaccac catagaaagg gccagcatca gcgtctggtt aacgcccgcc ataatggtcg
    10861 gcatcgccag cggtaactga actttgaaca gcatctggcg cgggctggca ccgaatgagc
    10921 gcgaggcttc aatcagatcc gccggaacct ggttaatccc cagaatggtc agacggataa
    10981 tcggcggcag agcaaagatg atcgtcacca ccacgcccgg cacgttaccg ataccaaata
    11041 gcatgacgat tggcaccaga taaacaaacg ctggcgtggt ctgcatggca tcaagcagtg
    11101 gacgaataat tttcgccgct cgcggacttc tcgccagcca gatccccagc ggcaaaccaa
    11161 tgacgataca gaacagcagg gcggttaaca ccagcgccag ggtaaccatg gcctgcgacc
    11221 aggcaccgat tgcgccgatg gcaatcagcg aaaccagcgt cgccacgccc attccgaccc
    11281 cggaaatctg ccaggcgatg agagcgaaaa cgataatcgc caccggtgcg ggcataccca
    11341 gcagcaattg ctggaaaccg ttgagaatgt aatcaaccgg aacgcgcacg ccctggaaga
    11401 cgggacggaa atgggtaaca acccagtcga tcccttcagt gacccaactg tcgagcggga
    11461 tcagcgtttt atggaacgga tcgagaatat taaaatgctc gacgtttggc gcaggcgtac
    11521 tggtcagcca gtcagcacca ccgccgtcag tcggtgcagt cgccggtgta ccccaggcgt
    11581 ctgcggattg cacggcactg tccgccgctg gcgtggtatc ccacggatta ttttgatcag
    11641 ccattattta ccccctcacg atctaaagcg cgcagcagca ttcctttcga aatgatgccg
    11701 acatactgtt ggtcctcgtc gaccacgggc accgcacagg gtgcctgtcc gacatgagag
    11761 agcaactcgc taagaggcgt ttgtgcatcg actgctaacg gcgcatcaat cagcgccgca
    11821 tcaagacctt gctgctgcgt taacgcggtt ttaagcgaat cgatggaaac tgcgccgaca
    11881 aacttattac cgcgttcgat aacgtagcca tattcgcgat cttcatcctg caataatttc
    11941 agtgccgaac gtgggccgaa gccaggggtt ttacgaatta agccattcgg tgtccggcgg
    12001 gcaatatctt tcgcactgaa tacctgacta atatcaacgc cacggaagaa ggtacggaca
    12061 taatcattcg ccggattatt gagaatttca tccggtgtgc cgacctgtac cacttcacca
    12121 ttttgcataa tggcaattcg gtcgccaata cgcatagcct catcgagatc gtgggaaata
    12181 aagacaatgg tgcgctgatg tttcgcctgt aattttacca gctcatcctg catctcggtg
    12241 cgaattaatg gatcgagcgc cgagaaggct tcgtccatta ataatatatc cggattaatc
    12301 gctaacgcgc gggctaatcc cacacgttga cgcatcccgc cagagagttc atccgggtag
    12361 ctgtgggcat aattttccag cccgacctga cgcagtgcat caagggcttt ttcccggcgt
    12421 tcttcggcat taattccggc caattccata ccgaacgcag tattgtccag cacggtcata
    12481 tgcggcatta aggcaaagga ctggaagacc atcgcaatct tttttctgcg cacctcacgg
    12541 agttcggcat cggatatttt ggcaatatcc acaccatcaa tcagcacttg cccgcgggtg
    12601 ggttcaatca ggcgattgag aaggcgtacc attgtggatt tacccgagcc ggataatccc
    12661 atgatgacaa atatctcgcc ttcttcaatg gccagactgg cgtcttttac gccaagcgat
    12721 agcccagttt tttccagaat ttgttctttc gaaagtcctt gttcgatata tttgaacgct
    12781 cgctgtggat gctcgccaaa tattttataa agatttttaa tttctaattt aattgccatg
    12841 caatagaaag attcctttat ttgtttatgt cgatatatta cccaatggaa atagtcactt
    12901 ttttctaccc taacatactg agaatctgag gcaacccctg agggcaaatg tggcatgaat
    12961 attgatgcga gcaaatcgcc ggaatttccc gtgatataag ggctgagagc aaatcgagaa
    13021 aagttgatat tttttgtcat agatagcgaa aaaacgcctg aatattgtag tttcaggcga
    13081 ataatgcaac agagtatcag tgtagatcac tacagaatat ttgcgtgata aaaagctatt
    13141 tggcgggaat tatttccctg ctgcgggtag tgatattttt gaaaataaca ccctcagaaa
    13201 ttccagtctt catcttctgt ttcaaccgct ttccccatca cataagagga gcctgaaccg
    13261 gaaaagaaat cgtgattttc atcggcattc ggcgaaagcg cggcgaggat tgccggattc
    13321 acttccgcca tttctgcggg aaataacggt tcgtagccca gattcatcaa agccttattg
    13381 gcgttgtaac agagaaacgc tttcacatcg tcagcccacg gggtttcggc gtacagctca
    13441 tcggtgtatt gcaactcgtt gtcgtagagt tccagcaaca aatcgaaggc gaaactcttc
    13501 aactcttcac gttgtcccag agatatcttt tccatgtttt tctgatattt atagcctatg
    13561 tagtaaccgt ggactgcttc atcgcggata atcagacgga tcagatctgc ggtattggtc
    13621 agctttccgc ggctggaaaa atacatcggc agccagaaac cggaatagaa caaaaaagat
    13681 tcaagaaaca cactggcgat tttctttttc agcggatcat caccgcgata atgttgctga
    13741 ataatctgag cttttcgctg caacggtgcg ttttcttcac tccaggcgta ggcggcatcg
    13801 acatctttgg tctgacacag cgtcgagaaa atcgaactgt aagagcgggc atgaaccgct
    13861 tccataaagc tgatattcga taatactgcc tcttcatgag gcgtgagcgc atcgggcatc
    13921 agcgaaggcg cgccgataac attttgcagc gtgtcgagca gcgtcaggcc agtaaaaacg
    13981 cgcattgtca gttgttgttc tacgacactt aatgtctgcc atgcaggtat atcgttcgac
    14041 agtggcacct tttctggtag ccagaaattg ctggtcaggc gattccacac ctccagatct
    14101 ttatcgtcag atatcttgtt ccagttgatg gcgctgatac gtgagagttt catagatatt
    14161 ccttaaagtg cacaggagac gcagccttca atttcagtgc cttccagcgc catctgacgc
    14221 aggcgaatgt aatagagcgt tttgatacct ttgcgccagg cgtaaatctg cgctttgttg
    14281 atatcgcgag tggtggcggt atcggggaaa aacagcgtca gcgacaaacc ctgatcgaca
    14341 tggcgagtcg cttccgcgta ggtgtcgatg atcttttctg cgccgatttc gtaagcatcc
    14401 tgatacagcg ccagattctc gttagtcata aacggggcag ggtagtaaac gcgtcctgtt
    14461 ttgccctctt tgcgtatctc tactttcgcc acaatcggat gaatactcga cgtagcatgg
    14521 ttgatataag agatagaacc ggttggcggc accgcctgga gattctggtt gtatatgccg
    14581 tagcgcatca cgtcgtcgcg cagttgcgcc cacatctcac gggtaggtaa cgtaataccg
    14641 ctgcgggcaa acagttcgcc aactttcgcc gttttcggct gccagttacc ttgcagatat
    14701 tggctgaaat attcaccact ggcatagcgt gactgtttga acccggcgaa ggtttcaccg
    14761 cgttcgcgcg ccagcaacat cgaggtacgc agtgcgtgcc aggtgatggt atagaaatag
    14821 agattggtga aatccagggc ttccggcgaa ccataagcga tgccttccct cgccagataa
    14881 ccatgtaaat tcatctgccc cagtccgatg gcgtgcgagg cggcatttcc ggcttcgatg
    14941 gacggcacgc tgcggatatg actcatatct gacaccgccg ttaaaccgcg tacggcagtc
    15001 tctaccgtgc gggcaaagtt gggggaatcc atggtgtggg caatattcaa cgaacctaaa
    15061 ttgcaggaaa tatcatggcc tgtacgggca tagtcgagat tctcgtcata ctctgaggcg
    15121 ctgttaacct gcaaaatttc tgagcagaga ttactcatat ttatgcgccc ggcgataggg
    15181 ttagcacggt ttaccgtgtc ttcatacatg atgtagggat agccggactc aaactggatt
    15241 tctgccagtc gctggaagaa atcacgggcg ttgaggtatt ttttgcggat gcgttcatcg
    15301 gcaaccagtt cgtcatagtg ttcgctaatg gcgatatcgg caaacggctt gccataaact
    15361 cgctcaacgt catagggcga aaacagcgcc atctgcgcat tctcttttgc cagatggaaa
    15421 gtgatatccg ggatcaccac gccaagcgac agtgttttaa tgcggatttt ttcgtcggca
    15481 ttttcccgtt tcgtgtcgag aaaacgcaga atatcgggat gatgagcatg taaatagact
    15541 gcaccagccc cctgacgcgc gccgagttgg ttgggatagg aaaatgcgtc ttccagcatt
    15601 ttcatcaccg gaattacgcc agaagattga ttttcaatac gtttaattgg cgcgcccgct
    15661 tcccgcagat tcgacagcaa aaatgccacg ccgccgccgc gtttcgacag ttgcagtgcg
    15721 gaatttaccg cccggccaat cgactccata ttgtcttcaa tacgcagcag aaaacaggaa
    15781 accagttcgc cgcgctgctg cttaccgcag ttgaggaatg ttggcgtggc tggctgaaag
    15841 cgtcctgaca gcatttcatc ggtcagttgc aacgccagcg tcgcatcgcc ttgtgccagc
    15901 gtcagcgcca ccatcgtgac tcgatcggca aaatcttcca gataacgttt accgtcgaat
    15961 gtcttcaacg tatagctggt gtaaaacttc catgccccga ggaatgtctg gaaacgaaaa
    16021 ccgctggtgt gtgcgtgggc aaacagcgta atgacaaagt cgcgagagta gcgattaaga
    16081 acgctttcat catagtaacc ttcgttgacc agccagttca ggcgctgctg ctggctactg
    16141 aaggtcacac tgttcggacg cacatgcgtc gcaataaagg cgtcaacggc ctggcggtct
    16201 ttatcgaact gaatgcgacc tgcgctatcg tagaggttaa gcatcgcatt cagcgcgtgg
    16261 taatccatcg tttcctgcgt caggcattct gcggttgtcg ttgccaaaat tcggttactc
    16321 ctttacgaac gttttcgata tcgctttgcg tacccatgag ttcaaaacgg tacagccacg
    16381 gcacgccgca tttccgggca atcacatctc cggcgcggcc atacgcctca ccaaagttgc
    16441 gattaccaga agcaataacg ccgcgaagca acgcccggtt gtgctcgtcg tttaaaaagc
    16501 gaattacctg tcgtggcacc gcgccagccg taccgccgcc gccgtaagag ggcacgatca
    16561 ggatgtaagg ctcgtctacc tgaatccgtt cccgctcatt gagcgggatg cgcaccgcgg
    16621 gcagacctaa acgttcgata aaacgctgcg tgttttcgga gctgctggag aagtagacga
    16681 gctggctcat gcactggccg cgtgtggcgc tggatgcaga cggttaatca tgtccggacg
    16741 gaaaccagac cagctaagat cgccagcaat cactaccggc aactgacgaa agccctgagc
    16801 acgcaacgct tccgctgctt caggaacgcg atcgacatta atcatttcaa aatcaaagcc
    16861 ccggttttcc atcgcccgtt tggtggcgtg gcactgaacg caatcgttac gagtgtaaat
    16921 agtaatgcgc atggttcgta tttccgttta aaatgaagat acggcgcgat gatacgcgtc
    16981 gggttgtctc tctgttgata cagagatact agatgtagtt gaaaaaagat tcaaccacac
    17041 aatatatagc aaatagtggt cgaaattacc ctggatatga gcgtgacggg gtagggggat
    17101 ttttgtgatt caccaggcaa aaagaaaccc cgaagccagg cttcggggtc aaagacgcgt
    17161 atttattatc atttttgcac tacgatttgc gcatgcttaa cagtgcgccg ataaaaatac
    17221 ctaccgcagc tgctgtaccc acgctacacc agggtctttc acgaacaaaa gaatccgcgc
    17281 agccaacggc atcgcgtgcg gcttgctgga cgcgagtacg accatgcatt cgcgccctgg
    17341 tttctttcag caatgcctga gctttactgc gtgcggcttc agcttcccct ttggcgtcgc
    17401 tgccccagga cttcaataca gattcgaggc tgtcagctaa ttgattgaca tcattctgga
    17461 tatcctgcac gccatcatca acgtcgttgc ggttcggtcg gttaaacata tgatccccca
    17521 ttagaattcg atgtaatttt cagtgtagac cataattttc ttaactgagg ttcatcgcgg
    17581 ctttatggac ttttctgaaa gcgttgtgaa tgcgcaatca ggtaaggtag gcccgcggtt
    17641 gataataacg aggatcaaat atgtatttac gaccagacga ggtggcgcgc gtacttgaaa
    17701 aagtcggttt tactgtcgat gtggtaaccc agaaagcgta tggttaccgc cgtggggaaa
    17761 attatgtcta tgttaatcgc gaagcgcgaa tgggacgtac cgctctggta attcatccaa
    17821 cattaaaaga acgcagttca acactggctg aacccgcttc cgatattaaa acttgcgatc
    17881 attaccagca atttccgctc tatttagcag gcgagcgaca cgagcattac ggtatcccgc
    17941 atggctttag ttcgcgtgtt gcgcttgaac gttatttgaa tggtttattt ggcgaagcca
    18001 gttaaagacg cgactggcga tgccagtcgc gaaaagaaga gtcaggcttt tacctgctgg
    18061 taacggctga ctttaaacag tcggcggcaa taatcgagga agtagccata aaccgccccc
    18121 atcaacatcg aaacaacgat gtttgaactg accgccgcca taatctgatg ccagtctgcg
    18181 cccaccacta acaagatcgc cacatacacc ggtgactgga acgtcacata agccaggata
    18241 tcagccagat tttttatcca gcccgacggg ctaactttgc gtgccgcgcg cataaacaga
    18301 tcgcggtaca taccgtatgg ccatgcaatt aagatgttcg ccggaatcgc taccaatctg
    18361 gaataaaaag actgttcgaa gctcattccg gagaggaaaa cttcaataca catgttcacg
    18421 acagaacagt aaacaaccat cgcgaacgta tctgcaactg catgacgcaa gcgtgactgc
    18481 ggtgagaaca tgctaatgct ccttaataaa agcgtaatgg gatgttaatg gagatgaaag
    18541 cagtggatct cattgccatc tgcattttcg catagcgtat atcgctggct atgactattc
    18601 aattagtgaa aaatttttat ttagtcagtt tttgtagata aaatattccg taacggctcg
    18661 ttttttgtat actgtggatt tttacatgcc gatatgattt attttcaatt aaatcaattg
    18721 gatatgacct tgcctttaat ttcactaatg aaagtttggg ggtgaggtaa cgctataagc
    18781 gtagagaatt cggtgtatgt cgtgaccggg tcatttacag cattttttat gccagaaaat
    18841 gtacattaac agcagaaagc accagtgagg ggtgttttct tttattctgt tatattgcat
    18901 ttttattcac ttcggttttg tcaggcttgc ggaattagcg agcagagagc accactctgt
    18961 tcaccaataa tct
//
LOCUS       DAZFWL020000086        18968 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFWL020000086 DAZFWL020000000
VERSION     DAZFWL020000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.086"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..658)
                     /locus_tag="ACHKNP_RS16090"
                     /old_locus_tag="pgaptmp_003218"
     CDS             complement(<1..658)
                     /locus_tag="ACHKNP_RS16090"
                     /old_locus_tag="pgaptmp_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU4705335.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     F"
     gene            complement(669..1670)
                     /gene="ltaE"
                     /locus_tag="ACHKNP_RS16095"
                     /old_locus_tag="pgaptmp_003219"
     CDS             complement(669..1670)
                     /gene="ltaE"
                     /locus_tag="ACHKNP_RS16095"
                     /old_locus_tag="pgaptmp_003219"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HGU4705336.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1707..3425)
                     /gene="poxB"
                     /locus_tag="ACHKNP_RS16100"
                     /old_locus_tag="pgaptmp_003220"
     CDS             complement(1707..3425)
                     /gene="poxB"
                     /locus_tag="ACHKNP_RS16100"
                     /old_locus_tag="pgaptmp_003220"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HGU4705337.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3558..4526)
                     /gene="hcr"
                     /locus_tag="ACHKNP_RS16105"
                     /old_locus_tag="pgaptmp_003221"
     CDS             complement(3558..4526)
                     /gene="hcr"
                     /locus_tag="ACHKNP_RS16105"
                     /old_locus_tag="pgaptmp_003221"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HGU4705338.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4538..6190)
                     /gene="hcp"
                     /locus_tag="ACHKNP_RS16110"
                     /old_locus_tag="pgaptmp_003222"
                     /gene_synonym="priS"
     CDS             complement(4538..6190)
                     /gene="hcp"
                     /locus_tag="ACHKNP_RS16110"
                     /old_locus_tag="pgaptmp_003222"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HGU4705339.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6334..7233)
                     /gene="lysO"
                     /locus_tag="ACHKNP_RS16115"
                     /old_locus_tag="pgaptmp_003223"
     CDS             complement(6334..7233)
                     /gene="lysO"
                     /locus_tag="ACHKNP_RS16115"
                     /old_locus_tag="pgaptmp_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HGU4705340.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTSLAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(7728..8423)
                     /gene="aqpZ"
                     /locus_tag="ACHKNP_RS16120"
                     /old_locus_tag="pgaptmp_003224"
     CDS             complement(7728..8423)
                     /gene="aqpZ"
                     /locus_tag="ACHKNP_RS16120"
                     /old_locus_tag="pgaptmp_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HGU4705341.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            8849..10507
                     /gene="ybjD"
                     /locus_tag="ACHKNP_RS16125"
                     /old_locus_tag="pgaptmp_003225"
     CDS             8849..10507
                     /gene="ybjD"
                     /locus_tag="ACHKNP_RS16125"
                     /old_locus_tag="pgaptmp_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HGU4705342.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HSSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(10504..11460)
                     /gene="ybjX"
                     /locus_tag="ACHKNP_RS16130"
                     /old_locus_tag="pgaptmp_003226"
     CDS             complement(10504..11460)
                     /gene="ybjX"
                     /locus_tag="ACHKNP_RS16130"
                     /old_locus_tag="pgaptmp_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HGU4705343.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            11611..12726
                     /gene="macA"
                     /locus_tag="ACHKNP_RS16135"
                     /old_locus_tag="pgaptmp_003227"
     CDS             11611..12726
                     /gene="macA"
                     /locus_tag="ACHKNP_RS16135"
                     /old_locus_tag="pgaptmp_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HGU4705344.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            12723..14669
                     /gene="macB"
                     /locus_tag="ACHKNP_RS16140"
                     /old_locus_tag="pgaptmp_003228"
     CDS             12723..14669
                     /gene="macB"
                     /locus_tag="ACHKNP_RS16140"
                     /old_locus_tag="pgaptmp_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HGU4705345.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(14742..14966)
                     /gene="cspD"
                     /locus_tag="ACHKNP_RS16145"
                     /old_locus_tag="pgaptmp_003229"
     CDS             complement(14742..14966)
                     /gene="cspD"
                     /locus_tag="ACHKNP_RS16145"
                     /old_locus_tag="pgaptmp_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HGU4705346.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            15116..15190
                     /gene="yljB"
                     /locus_tag="ACHKNP_RS16150"
                     /old_locus_tag="pgaptmp_003230"
     CDS             15116..15190
                     /gene="yljB"
                     /locus_tag="ACHKNP_RS16150"
                     /old_locus_tag="pgaptmp_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HGU4705347.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            15289..15609
                     /gene="clpS"
                     /locus_tag="ACHKNP_RS16155"
                     /old_locus_tag="pgaptmp_003231"
     CDS             15289..15609
                     /gene="clpS"
                     /locus_tag="ACHKNP_RS16155"
                     /old_locus_tag="pgaptmp_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HGU4705348.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            15640..17916
                     /gene="clpA"
                     /locus_tag="ACHKNP_RS16160"
                     /old_locus_tag="pgaptmp_003232"
     CDS             15640..17916
                     /gene="clpA"
                     /locus_tag="ACHKNP_RS16160"
                     /old_locus_tag="pgaptmp_003232"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HGU4705349.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            18433..>18968
                     /locus_tag="ACHKNP_RS16165"
                     /old_locus_tag="pgaptmp_003233"
     CDS             18433..>18968
                     /locus_tag="ACHKNP_RS16165"
                     /old_locus_tag="pgaptmp_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4705350.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWG"
BASE COUNT         4704 a         4724 c         5124 g         4416 t
ORIGIN      
        1 caaactgttg ctgataactg agcacctctg gtccggcggc ttcgaagatg cggtgttcgc
       61 tggccggatg atctaacaac gccaccagat agtgcagcaa gttttccagc gcgatgggcg
      121 tggtgcgtga acgtacccag cgtggcggcg ttaacactgg caggttgtag accatatcgc
      181 gcatgacttc gaacgccgct gaacctgcgc caacgataat tccggcccga agttcggtca
      241 caggtacatt cgcttcacga agaatgtccg ccgtagcctg acgagcacgc agatgatccg
      301 actgctcatg tggcggggcc tgcaacgaac tgagaaagat taattgctta actggtactt
      361 cacgtagcgc atcgcggacg ttgagagcca cctggcgctc ctgagcgata aaatcgccgc
      421 cttcgcccat gctgtgcacc agaaaataga cggtatcgat atcctgcaac agggccggaa
      481 ggttatccgg ccagctgaga tcgactttat ggcaactgac gtttgccagt tgcagctttg
      541 caagcctgtc gacatgacgt gccgccgcca ggatctgatg cccttgctgg ctgagtgtgc
      601 gcaccagatg ctgaccaatg tagccactgg caccgagaac taaaatgcgt tgcggcacgt
      661 ctctctcctt aacgcgccag gaatgcgcgc cagtgggcgg cgacttccgc cagttgttcg
      721 cgcgagacgt caagatgcgt caccaggcgg acaatcggcg aggcgttaat cagcacgttt
      781 ctcgctttca tgtattcgcc taacgcggca gcattttctt ccccgacgcg aacaaacagc
      841 atattagtgt cctgacgcat cacatccgcg cctgcttcac gcagttgctc cgccatccag
      901 gcggcgttgt cgtgatcttc ctgcaaccgt gcgacgttat ttttcagggc atacatcccg
      961 gcggcagcca gaatcccgga ctgacgcatc ccgccaccgg ccattttccg ccagcggatg
     1021 gcacgtttaa tgtaatcacg attaccgacg agtaatgaac cgactggcgt cccaagacct
     1081 ttcgacaggc aaatggtgaa cgaatcacaa tattgcgtga tctctttcag ttcgcagccg
     1141 taagccacca cggcattaaa gatgcgcgca ccgtcaacat gcagcgccag attgcgctcg
     1201 cgggtaaatt cccatgcttc tttcaggtat tcacgcggca gcactttgcc gttgtgggtg
     1261 ttttccagac tgagtaattt ggtgcgggcg aaatggatat cgtcgggttt gattttcatc
     1321 gccactttat ccagcggtag cgtgccgtcg gcagccgcgt cgatgggttg cggctgaata
     1381 ctgcccagca ccgctgcgcc accggcttca aacagatagt tatgcgcggc ctgaccgaca
     1441 atatactctt cgccacgttc gcagtgactg agcagagcga ccaggttggc ctgagtgccg
     1501 gtaggcagaa aaatggcggc ttctttaccg gaaagctctg ctgcatagtc ctgcagagca
     1561 ttaacggtag ggtcgtctcc gtaaacgtcg tccccaaccg gggcggccat catcgcttcg
     1621 agcatggcgc ggctcggtcg ggtaacggta tcactgcgta aatcaatcat ggcatgtcct
     1681 tattatgacg ggaaatgcca ccctttttac cttagccagt tcgttttcgc cagttcgatc
     1741 acttcatcgc cgcgtccgct gataattgcg cgcaacatat acaggctaaa acctttggcc
     1801 tgttcgagtt tgatctgcgg tggaatggct aactcttctt tggcgaccac cacatccacc
     1861 aacaccggac cgtcgataga gaaggcgcgt tgcagggctt catcaacttc agacgctttt
     1921 tctacacgga tacccgtaat gccgcacgct tcggcaatgc gggcaaagtt tgtgtcgtgt
     1981 agttcggtgc cgtcagtcaa atagccacca gctttcatct ccatcgccac aaagcccagc
     2041 acgctgttgt taaagacgac aattttcact ggcagtttca tctgcactac tgagaggaaa
     2101 tcgcccatca acatgctaaa accgccatcg ccgcacatgg cgaccacctg acgttctggc
     2161 tctgtcgcct gcgcacccag cgcctgcggc atggcgttag ccatcgaacc gtggttaaac
     2221 gaacctaaca ggcgacgctt gccgttcatt tttagataac gtgccgccca caccgttggc
     2281 gtaccaacgt cacaggtgaa aatagcgtca tcggcggcaa aatgactaat ttgctgcgcc
     2341 agatattgcg ggtgaatggc tttctcgctc ggtttagcta aatcgtccag ccctttgcgg
     2401 gcgtcgcggt aatcttccag cgctttatcc agaaacttgc gatcggcttt ttcttccacc
     2461 aatggaagca atgcacgcag agtcgacttg atatcgccga ccagtgccat atccaccttg
     2521 ctgtgagcgc cgatgctggc tgggttgata tcaatctgaa tgattttggc atcggtcggg
     2581 tagaaggcgc ggtagggaaa ttgcgtgccg agtagcacta acgtgtcggc gttcatcatg
     2641 gtatggaaac ctgacgagaa gccgattaac ccggtcattc caacatcata cggattatcg
     2701 tattcgacat gttctttacc gcgcagggca tgaacaatag gcgctttaat tttcccggca
     2761 aactcaacta actctttatg cgcccccgcg cagccgctgc cacacatcag ggcgatattg
     2821 ctggaataac gcagcagttg cgccagtttg cgtaactctt cttcttccgg cgtcacgact
     2881 ggttgtggcg catgatacca gtgcgtggtt gccccttctg gcgcaggttt taacgccacg
     2941 tcgcctggta acacgacaac cgaaacgcca cggttaagca ccgctttgcg catggcaatc
     3001 gccagtactt gtgggatctg ctccgggctg gaaaccagct cgcaatagtg actacattcg
     3061 cggaatagct cttgtgggtg ggtttcctgg aaatagccgc tgccaatttc gctggaggga
     3121 atatgagcgg caatcgccag taccggaacg tgattgcggt ggcaatcgaa caggccgttg
     3181 attaagtgca ggttgccggg gccgcacgat ccggcgcaga ccgccagttc tccgctaagt
     3241 tgtgcttcag cgccagcggc aaaggccgcc acttcttcgt ggcgggtgga catccactcg
     3301 atggtgccca tgcgattaag actgtcacta agaccgttca gagagtcgcc tgtgactccc
     3361 cagatgcgtt tcacccctgc cgattcgagt gttttggcga tataagctgc aaccgtttgt
     3421 ttcatggttc tccatctcct gaatgtgata acggtaacaa gtttagttca tctgacggag
     3481 ggggaaggga tgggagagaa aggaggcact aacggttaaa tagcccgatg aaaggaatat
     3541 catcgggcat aaggcgatta tgcgagaacc aaatccccct gcggatggca ggagcaggcc
     3601 agtacgtaac cttcagcgat ttcggcgtcg gtcagcgtca ttgtactgct caccgtatat
     3661 tcaccggaaa ccacttttgt cttacagcag ccgcaaacac ccgcacggca ggcagcgaca
     3721 accggaacgt tattgctttc cagcgcctcc agtagcgtgg tgccaaccgg ggcgtaaaat
     3781 tctcgtgccg gttgcagttt ggtgaatttc agaccgctgg tcgccgcttc tgctactggg
     3841 gtgaagaatt tctctttaaa gaaacgcgtc acgccgagcg ctttcacttc ctgctctacc
     3901 caatccatat acggagccgg gccgcaggtc atcacggtac gtgaagctaa gtcaggtaca
     3961 cctgccagca gttcgcgagt gagacgacca gcgataaagc cttcggtaac gttattttct
     4021 gccaccagcg ttaccggata gttacgccac tcatcggcga aaataacatc ctgcggcgta
     4081 cgcacgttgt agatcacccg cacatcggcc tgtggacggt tcttcgcaag ccagcgacgc
     4141 atcgacataa tcggcgtgac gccgcagcct gccgccagca acaggaattt atcttctgct
     4201 ttatcgtcgc aggtaaattc ccccatcgcg tccgaaagcc agagataatc accgcgtttt
     4261 acatcgcgcg tcagccactg ggagccgaca ccgtcatcaa tccgccgcac ggtcagggtg
     4321 atatattcac tcacgcctgg cgtggaggaa atggtgtaag cacgcagcgt ttccgctgag
     4381 tttcgcacgc tgaccagtgc atattgcccg gcgcgatatg ggtagtaatc gtggcaaatc
     4441 agggaaatcg tccacacatc cggcgtttct tgcgtaatgt gatgaacctg catccgccac
     4501 gggcattgat tcgttggcat cgtcatcgac aaactcctta cgcgctcaac agttgcttca
     4561 tgtcttcttc aacagtggtg atagaacgca ggccaaattt ctcgttcagc accgccagca
     4621 ggtctggtgt caggaaacca ggtgcagtcg ggccggtgac gatatttttc acgcccagag
     4681 aaagcagcgt cagcagaatg acgatcgctt tctgttcaaa ccaggagagc accagcgaca
     4741 gcggcagatc gttgacaccg cagcccagtt tctctgccag agtgacagcc agaataatcg
     4801 ctgagtaagc atcgttacat tgacctgcat ctaccagacg cggcagacct tcgatatcgc
     4861 caaactcaag tttgttaaag cgatatttac cacaggcgag ggtcaggatc aggcagtcat
     4921 ccggcacgct ggtggcgaaa tcggtgaagt agtggcgctc gccgcgtgcg ccgtcacagc
     4981 caccaagcag gaagatatga cgcagttttt cacggctcac cagatcaatc agcgtatcag
     5041 cagcgccaag cagcgtctgg cgaccaaaac ccacggtgat aaggtgcgga atttcgctgt
     5101 acgggaagcc tgccatctgt tgcgcctggg tgataaccgc agagaaatca tcaccgtcca
     5161 gatgacgcac gccaggccag ccaacaatgc tgcgggtcca gatacgatcg tcataagcgc
     5221 ctacggttgg gtcgatgatg cagttcgagg tcatcacgat ggggccaggg aaacgagcga
     5281 actccacttg ctgattctgc cagccactgc cgtagttacc gaccagatgc ttgaatttac
     5341 gcagctccgg gtagccatgc gcaggcagca tttcgccgtg ggtgtagaca ttaacgcccg
     5401 tgccttcggt ctgttccagc aggttgtaga gatctttgag atcgtgaccg gaaatcagaa
     5461 tgcatttacc cgccgtcgct ttgacgttga cctgggttgg cgtcgggtga ccgtatttac
     5521 cggtttcgcc tgcatccaga atgctcatca ctttgaagtt catctggccg atttccattg
     5581 aacactcaag aagcgcgttc atatcggcag gccaggtccc cagccacgcc atgattttat
     5641 gatactgggc ataaatatcg ttgtcgtatt gaccgagaac atgcgcgtgt tccatatagg
     5701 ccgccgcacc tttcaggcca tacaggcaca gcagacgcag gccgagaatg ttttcgccaa
     5761 tcgccgcttt atctttgtta ggggtaaatt ctgcagcctg acgttgcagc tcgccgagat
     5821 cgtcgctcac cagttgcagg tcagccatcg ggttatcgac gcgcgcgttg gcatctacag
     5881 ccaggcattg cgctttcagc gcctcgcgca gggcaatcgc ttcacgagcg tagccgacaa
     5941 tacgcggaga atcgaagtta acgttggtca gagttgagaa aaaggcacgt ggcgcgaagc
     6001 tgtcaacatc gtggttgatg atgccgtatt cacgcgcttt taccgcccag gcagaaagcc
     6061 cttgcagcgc cgcgatgagt aaatcctgaa ggtcagaagt ttccgccgtt ttaccacaca
     6121 tcccctgcgc gtatgagcag ccgtttcctg ccggagtacg gatagtttgt tcacattgca
     6181 cacaaaacat gatcacacct tttaaagtta tatttaatat acatgtttaa ggttaagacg
     6241 cttaacgcgg ggataaaagg gatttttcat gcaactttaa gggagattga tttagcgcaa
     6301 ttttggcggc aggaatctac cgccagagag gtattacgca gagaaaaagg cgatgaggat
     6361 cggcactaac aggctaagaa taaaaccgtg aacaattgcc gccgggacca tatccagccc
     6421 gccagtacgt tgaagaacgg gcagggtgaa atccattgat gtggcaccgc ataagcccag
     6481 tgcagtagag cggctgcggc gaatcagccc agggatcaac ataatagcaa tcagttcacg
     6541 ggccagatca ttaaaaaacg ccgcgctccc gattaccgga ccaaaagatt cggtcaataa
     6601 aataccggaa agagaatacc agccgaaacc ggaggccatt gccagcgagg tattgatggg
     6661 gagatcaaga ataaaggcgt taattaaacc accaattaat gaactgacaa ccaccaccac
     6721 ggcgacaatc attccccggc gattaaggac aatctgcttt aaggtcatgc cattattgcg
     6781 caactgaata ccaacgagga aaagtagcaa aattaacgtg tattcactgg cttcggtcgc
     6841 gtgttgtaag aaagccagtc cactcagacc aatggcaaaa ccaatcacta ctacgccgca
     6901 cagttttagc gactccagcg ccatcgcaat acgcgacggg agtttttctt gctgatggtg
     6961 gttgcgccac ggcaggcctc gctccagcca catcagggcg gcaatattac acagtaaaat
     7021 aacggtaata ctgacggcag aataatgcag aatcgccaac aggttactgg cgaggttatc
     7081 gaggaacgcc agacttatac ccataaaaaa gagaataagg taaaccatcc agcttaatag
     7141 ctgattaata acttttaacg cagcttgttg gcgaagcgga atgaggtaac ccacaatcag
     7201 gggaaccaga atgattaaca gcccagaaaa catgaaaacc cagtccttgc aaagatgaag
     7261 tcgaaatgcg cgatgacaca ctactgaaag cggaaggacg agtaaagttg caattaaaag
     7321 gaaatgttat gcataaggag cagtagagta ttcgttttca tttaaagata ttcttgcgct
     7381 ttaattacaa actgcaccga tgttggtggc gtcaaaatcg ccgaggcgtt ccctgaaggc
     7441 cggggcagcc cacatggatg tgggctgagg gcgcgtttta cagggatgtt acctcgcgcc
     7501 cgacccggta gccgtaaggg ataagtcgag ggcaccgcgc agcggcgatt ttgttcgcca
     7561 gagcccgggg gtgcatgggg cggcggcgat tggccgcccc ctgcgcgctc cttgcgccag
     7621 tggcaatatg tggcttagct catgaaagga gcgcaacaag atgatgaatc aacatataac
     7681 aacatcttaa aaaaaggcct gacattacgc caggccttct gcgttaatta atcacgcttt
     7741 tccagcaggg tccggtaaat cagaccaccg ataatgccgc cgacaattgg caccacccag
     7801 aagaaccaca gttgttctaa tgcccagccg ccctggaaga tagcaaccgc ggtgctgcgc
     7861 gccgggttaa cagaagtgtt agtcaccgga atactaatta agtgaatcag ggttaaggcc
     7921 agaccaatag cgatcggcgc aaaacctgcc ggcgcgaatt tgtcggttgc gccgtggatc
     7981 accaacagga aacctgcact caataccagt tcaactacca gcgcggaaag catggaataa
     8041 ccgcctggtg aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc cgcgtcaaaa
     8101 cccgttttac cactggcaat taaatacagc agcgccgctg caacaatacc gccgacaacc
     8161 tgggcaatta cgtagccaac gacttctttt gccggaaaac gtccgccagc ccataaacca
     8221 atagtgaccg ccgggttaaa atgaccacca gaaatatgac caacagcaaa ggccatcgtc
     8281 agaacggtca gaccgaacgc caacgccacg ccggcaaaac caatgcctaa ttccgggaag
     8341 cctgcggcca gtacagcact accacagcca ccaaaaacaa gccagaaagt accaaaacat
     8401 tcagctgcta attttctgaa catatccacc tcaattaaaa attgaccctg tgaaaaatat
     8461 ggtcgtttta tagggtcgtc gtaaaaaagt gacgacggaa ataatgcgcg gctattttaa
     8521 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
     8581 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
     8641 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgca
     8701 gacttatcag caagagggag tataacgcga ttattcgctc atttttcaga catttgccat
     8761 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg ccagcgtaaa
     8821 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
     8881 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
     8941 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
     9001 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
     9061 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
     9121 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
     9181 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
     9241 cgataaagac ggacatccga ttgatgtcga ggatattaac gatcaggcac gccatctggt
     9301 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
     9361 cacggtgcca aatgttccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
     9421 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     9481 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     9541 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     9601 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     9661 tactttattg caggcaaaag gcacattgag actggataaa gacgcgcgtc cgttattgct
     9721 aattgaagat ccagaaaccc gtttacaccc cattatgctt tcagttgcct ggcatctgtt
     9781 gaatcttctg ccattgcagc gcattgccac caccaactcg ggtgagttgc tttcgttaac
     9841 gccggtagag catgtttgcc gactggtacg tgagtcctcg cgcgttgccg cctggcgtct
     9901 ggggccgagt ggcttgagta ccgaagatag ccgacgcata tcctttcaca ttcgttttaa
     9961 ccgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
    10021 ggttatcaat gaactggcgc gccagtgtgg acaccatttc gatgccgaag ggatcaaggt
    10081 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
    10141 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
    10201 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
    10261 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
    10321 aatcccggaa aatgtaccga tgaatctgcg caaaattatc tcgaaagcga tccatagctc
    10381 ttccaaaccc gatcttgcca ttgaagtggc aatggaggcc ggacgtcgtg gtgtggactc
    10441 cgtaccgacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
    10501 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
    10561 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
    10621 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgatt cccagaaagc
    10681 gttgtaatcg gcatggatct tgccttcttt atcacgataa cgcaggctgc ggtaaatatg
    10741 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
    10801 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
    10861 ttcctgatgt gggatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
    10921 cccctgatat tcacacaggg taaaagtgag ctctgccaga ggaatacctt cgctgttgcg
    10981 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
    11041 gaactgctcg ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
    11101 taagctgaat tcttccgccg acatacatcc gcggagtaac gcataatggt aacgtaacgc
    11161 ctccagcaat tgcttacggc taagattcgc cgcaaggtaa gggcgatgca gacgcacagg
    11221 cagtcgcggt tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
    11281 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
    11341 acggcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
    11401 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
    11461 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
    11521 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
    11581 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
    11641 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
    11701 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
    11761 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
    11821 cagttgaaaa ctctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
    11881 gttattgatc ctgaacaggc tgaaaaccag attaaggagg tcgaagcaac gctgatggag
    11941 ctacgtgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
    12001 cgtcagcaac gtctggcaca aacgcaggcg gtttcacagc aggatctcga caccgccgcg
    12061 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
    12121 caggcctctc tcgatacggc taaaaccaat ctcgattaca ctcgcatcgt tgccccgatg
    12181 gccggggaag tcacgcaaat caccactctg caaggccaga cggtgattgc cgcacaacaa
    12241 gcaccgaaca ttctgacgct ggcagatatg agcaccatgc tggtaaaagc gcaggtttct
    12301 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt acttggcgat
    12361 ccactgacgc gctacgaggg gcaaatcaag gatgtactac cgacgccgga aaaggttaac
    12421 gacgctattt tctattacgc ccgttttgaa gtccccaacc ccaatggttt gctgcggctg
    12481 gatatgactg cgcaagtgca tattcagctc accgatgtga aaaatgtgct gacgatccct
    12541 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
    12601 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
    12661 gtcaaagggc ttgaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
    12721 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
    12781 aggttgaggt gctgaagggc atcaccctcg atatttatgc cggtgagatg gtagcgattg
    12841 ttggcgcttc aggttccggt aaatcgaccc tgatgaatat tctcggctgt ctggataagg
    12901 ccaccagcgg cacctatcgc gtcgccggtc aggatgttgc cacactggac gccgatgcgc
    12961 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttactttcgc
    13021 atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgctggtctt gagcggaaac
    13081 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
    13141 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
    13201 tgaacggtgg tcaggtaatt cttgccgatg aaccaaccgg cgcactggac agccattctg
    13261 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg tgggcatacg gtgattatcg
    13321 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
    13381 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
    13441 ttgtcaacac ggtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
    13501 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
    13561 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
    13621 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
    13681 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
    13741 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
    13801 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
    13861 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
    13921 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
    13981 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
    14041 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
    14101 ccgggcgagt tatgggccag tcgtggctga actccattac tgtcagggta aaagaaggat
    14161 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggaaaga
    14221 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
    14281 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
    14341 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
    14401 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
    14461 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
    14521 ttttcttacc cggctgggag attggttttt caccgttggc actgctgctg gcgtttctct
    14581 gctcgacggt caccgggatt ttatttggat ggttacccgc acgaaatgcg gcacgactgg
    14641 atccagtaga tgctctggca cgagagtaat ttttgagata aaaatgccag ccgatcgggc
    14701 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
    14761 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
    14821 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
    14881 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
    14941 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
    15001 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
    15061 aatttagaga attagggcga gccgtcaagc gtttgacagg ggccaggggg caggtatgaa
    15121 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
    15181 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
    15241 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
    15301 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
    15361 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
    15421 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
    15481 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
    15541 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
    15601 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
    15661 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
    15721 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
    15781 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
    15841 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
    15901 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggtaac cggtgcaaac
    15961 gttctggtcg ctatctttag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
    16021 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
    16081 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
    16141 cgtatggaga atttcacgac gaacctgaat cagcttgcgc gcgtgggcgg aatcgaccca
    16201 ctgattggtc gtgagaagga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
    16261 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc ggaaggtctt
    16321 gcctggcgaa ttgttcaggg cgatgtgccg gaagtgatgg ctgactgtac gatttactct
    16381 ctcgatatcg gttctctgtt agcgggcact aaatatcgcg gcgactttga aaaacgtttt
    16441 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
    16501 cacaccatta tcggtgcggg tgcagcgtct ggtggccagg tcgatgcggc taacctgatc
    16561 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
    16621 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
    16681 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
    16741 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcag cggtagagct ggcggtgaaa
    16801 tacattaacg atcgtcatct gccggataaa gccattgacg ttatcgacga agcgggcgct
    16861 cgcgcacgcc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
    16921 tccgtggtgg cccgtattgc gcgcattcca gagaagagtg tttctcagag tgaccgcgat
    16981 accctgaaaa acctcggcga tcgcctgaaa atgctggtct tcggtcagga taaagccatt
    17041 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
    17101 gttggttcgt tcctgtttgc cggccctacc ggggtcggga aaacagaggt gacggtacag
    17161 ctttcgaaag cgttgggcat tgagctgctg cgctttgata tgtccgagta tatggaacgc
    17221 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggaggt
    17281 ttgctgactg atgcggtcat caagcatcca catgcggtgc tgttgctgga cgaaatcgag
    17341 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
    17401 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac caccaacgcc
    17461 ggggtacgtg aaactgagcg taaatccatt ggtcttatcc accaggataa cagcaccgat
    17521 gcgatggagg agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
    17581 atctggtttg atcatctgtc aaccgacgtg atccatcagg tggtggataa attcatcgtc
    17641 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
    17701 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
    17761 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
    17821 ggcggtcagg tcaccgtcgc gctggataaa gagaaaaatg agctgactta cggattccag
    17881 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgttaggt aggttggtca
    17941 agtccgtaat ctcgaaagag gttacggact ttttgtttat ggggtggagg aggttcagac
    18001 ccttttttaa tgatgatggt aagttgttga taattagtgc tgcgggaagg taaggataaa
    18061 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
    18121 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
    18181 cgaacgcgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
    18241 cgagctcttc tttaaatatg gcggtgaggg ggaccgcctg aaataaatct gacttaatcc
    18301 aggggtgtta tataatttaa ttataaacaa tgtattacac tttttgttgt ccgatattat
    18361 ctaatgtatt cttagtgaag cgagagacta atggcatact ggatggcata ccagagtgac
    18421 gggaattagc gtatggcagt attgacggat acgaaagcaa gacatatcaa acctgatgac
    18481 aaaccattgc cccatggggg aattaccgga ctgacccttc atccttcttc agtaaagggg
    18541 cgggggaaat gggtttttcg ttatgtaagt ccggtgacac aaaaaagacg taatgctgga
    18601 ttgggaactt acccagaggt cagtattgct gaagctgcac gtactgcccg gataatgcga
    18661 gagcaacttg ctgcaggtga tgatcctctg gagattaaaa aggctgaatc tgagaaagtc
    18721 gctatcccaa catttgccga tgcagccagg cgtgtacatg cagaactgtc tcctggatgg
    18781 gaaaatccaa agcatgtaag gcagtggtta tcgacgcttg agaattacgc gtttcctcaa
    18841 ctgggagcaa aaacgctgga ttcgattacg gctgcggacg tggcagaaac actgcgtcca
    18901 gtctggttaa ccttgtcaga aacggcaagc cgggttaaac agcgcattca tgttgttatg
    18961 cagtgggg
//
LOCUS       DAZFWL020000087        18958 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFWL020000087 DAZFWL020000000
VERSION     DAZFWL020000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.087"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1023
                     /locus_tag="ACHKNP_RS16170"
                     /old_locus_tag="pgaptmp_003234"
     CDS             <1..1023
                     /locus_tag="ACHKNP_RS16170"
                     /old_locus_tag="pgaptmp_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4705351.1"
                     /translation="QNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGFYLTCVV
                     AVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQAKGIEP
                     LSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDIISKRRE
                     SLEDQWGIEDSESYCALMEYFLSGDHGANTFKANMEEAPEQVIALLNKFAVFPSDYIS
                     DCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKAHELFES
                     EEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIRNVPWGA
                     SSVKYS"
     gene            complement(1140..2483)
                     /gene="gdhA"
                     /locus_tag="ACHKNP_RS16175"
                     /old_locus_tag="pgaptmp_003235"
     CDS             complement(1140..2483)
                     /gene="gdhA"
                     /locus_tag="ACHKNP_RS16175"
                     /old_locus_tag="pgaptmp_003235"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGU4705352.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLERQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            2719..2991
                     /locus_tag="ACHKNP_RS16180"
                     /old_locus_tag="pgaptmp_003236"
     CDS             2719..2991
                     /locus_tag="ACHKNP_RS16180"
                     /old_locus_tag="pgaptmp_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HGU4705353.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(2957..3364)
                     /gene="nudG"
                     /locus_tag="ACHKNP_RS16185"
                     /old_locus_tag="pgaptmp_003237"
     CDS             complement(2957..3364)
                     /gene="nudG"
                     /locus_tag="ACHKNP_RS16185"
                     /old_locus_tag="pgaptmp_003237"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HGU4705354.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            3451..4071
                     /locus_tag="ACHKNP_RS16190"
                     /old_locus_tag="pgaptmp_003238"
     CDS             3451..4071
                     /locus_tag="ACHKNP_RS16190"
                     /old_locus_tag="pgaptmp_003238"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4705355.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            complement(4080..5387)
                     /gene="ynjE"
                     /locus_tag="ACHKNP_RS16195"
                     /old_locus_tag="pgaptmp_003239"
     CDS             complement(4080..5387)
                     /gene="ynjE"
                     /locus_tag="ACHKNP_RS16195"
                     /old_locus_tag="pgaptmp_003239"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HGU4705356.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(5454..6107)
                     /locus_tag="ACHKNP_RS16200"
                     /old_locus_tag="pgaptmp_003240"
     CDS             complement(5454..6107)
                     /locus_tag="ACHKNP_RS16200"
                     /old_locus_tag="pgaptmp_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU4705357.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            complement(6107..7642)
                     /locus_tag="ACHKNP_RS16205"
                     /old_locus_tag="pgaptmp_003241"
     CDS             complement(6107..7642)
                     /locus_tag="ACHKNP_RS16205"
                     /old_locus_tag="pgaptmp_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGU4705358.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPTLLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(7615..8781)
                     /locus_tag="ACHKNP_RS16210"
                     /old_locus_tag="pgaptmp_003242"
     CDS             complement(7615..8781)
                     /locus_tag="ACHKNP_RS16210"
                     /old_locus_tag="pgaptmp_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4705359.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(8791..9339)
                     /locus_tag="ACHKNP_RS16215"
                     /old_locus_tag="pgaptmp_003243"
     CDS             complement(8791..9339)
                     /locus_tag="ACHKNP_RS16215"
                     /old_locus_tag="pgaptmp_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGU4705360.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(9339..10046)
                     /locus_tag="ACHKNP_RS16220"
                     /old_locus_tag="pgaptmp_003244"
     CDS             complement(9339..10046)
                     /locus_tag="ACHKNP_RS16220"
                     /old_locus_tag="pgaptmp_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU4705361.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(10061..10738)
                     /gene="ydjY"
                     /locus_tag="ACHKNP_RS16225"
                     /old_locus_tag="pgaptmp_003245"
     CDS             complement(10061..10738)
                     /gene="ydjY"
                     /locus_tag="ACHKNP_RS16225"
                     /old_locus_tag="pgaptmp_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HGU4705362.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(10743..11453)
                     /locus_tag="ACHKNP_RS16230"
                     /old_locus_tag="pgaptmp_003246"
     CDS             complement(10743..11453)
                     /locus_tag="ACHKNP_RS16230"
                     /old_locus_tag="pgaptmp_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU4705363.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLLGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(11620..12426)
                     /gene="xthA"
                     /locus_tag="ACHKNP_RS16235"
                     /old_locus_tag="pgaptmp_003247"
     CDS             complement(11620..12426)
                     /gene="xthA"
                     /locus_tag="ACHKNP_RS16235"
                     /old_locus_tag="pgaptmp_003247"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGU4705364.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12872..14092
                     /gene="astC"
                     /locus_tag="ACHKNP_RS16240"
                     /old_locus_tag="pgaptmp_003248"
     CDS             12872..14092
                     /gene="astC"
                     /locus_tag="ACHKNP_RS16240"
                     /old_locus_tag="pgaptmp_003248"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HGU4705365.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQLGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            14089..15123
                     /gene="astA"
                     /locus_tag="ACHKNP_RS16245"
                     /old_locus_tag="pgaptmp_003249"
     CDS             14089..15123
                     /gene="astA"
                     /locus_tag="ACHKNP_RS16245"
                     /old_locus_tag="pgaptmp_003249"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HGU4705366.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            15120..16598
                     /gene="astD"
                     /locus_tag="ACHKNP_RS16250"
                     /old_locus_tag="pgaptmp_003250"
     CDS             15120..16598
                     /gene="astD"
                     /locus_tag="ACHKNP_RS16250"
                     /old_locus_tag="pgaptmp_003250"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HGU4705367.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            16595..17938
                     /gene="astB"
                     /locus_tag="ACHKNP_RS16255"
                     /old_locus_tag="pgaptmp_003251"
     CDS             16595..17938
                     /gene="astB"
                     /locus_tag="ACHKNP_RS16255"
                     /old_locus_tag="pgaptmp_003251"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HGU4705368.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            17931..18899
                     /gene="astE"
                     /locus_tag="ACHKNP_RS16260"
                     /old_locus_tag="pgaptmp_003252"
     CDS             17931..18899
                     /gene="astE"
                     /locus_tag="ACHKNP_RS16260"
                     /old_locus_tag="pgaptmp_003252"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HGU4705369.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         4470 a         4879 c         5132 g         4477 t
ORIGIN      
        1 caaaaccatc ctgggagcga gaagtattct tttaatggct gggaaatatt taatagtaat
       61 tttgaacgga tgattaaaga aaataaggcc atgctgcttt gtaagtgggg gttttattta
      121 acatgtgttg tcgctgtaat gtttgtgttc gcagcgataa catccaacgg tttgaatgaa
      181 agaggcctga ttaccgcggg atgctctttt ctttatctat taattatgat ggggcttatt
      241 gttcgggccg gttttaaagc aaaaaaagaa caactgcatt attatcaggc taaaggtatt
      301 gagccgctca gtatcgagaa gttacaggcg ctacaattga tcgcacctta tcggttctat
      361 cataagcaat ggtctgaaac gctggagttc tggccgcgaa agcctgaacc tggcaaagat
      421 accttccaat atcatgtgct tccctttgac tcgatcgata tcataagtaa aagacgcgag
      481 tctttagagg atcaatgggg tatcgaagat agcgaaagtt attgtgcctt aatggagtat
      541 tttctttctg gcgaccatgg agccaatacc tttaaagcaa acatggagga agccccagag
      601 caggttatcg ccttgttgaa taaatttgct gtttttccct cagactatat ctctgattgc
      661 gctaataatc gctccggtaa atcctcggcg aagctaatat gggcggcgga attatcatgg
      721 atgacctcga tatcaagcac agcttttcaa aacgggacaa ttgaagaaga actggcctgg
      781 cattatataa tgcttgcttc tcgaaaggcg cacgagttgt tcgaaagcga agaagattat
      841 caaaaaaata gtctaatggg gtttctttac tggcatatct gctgctatcg cagaaagtta
      901 acggatgctg aacttgaagc gtgttatcgt tacgacaagc agttttggga gcactacagt
      961 aagaaatgcc gttggcccat aagaaatgtt ccgtggggag catcatccgt taaatactca
     1021 taaacgcctg aaattttgcc gggggcgctg cgcttgcccg gcctacatta gagcgtaact
     1081 gcaatgaatt gtgcccattt gtaggcctga taagcgtagc gccatcaggc atttacaact
     1141 taaatcacac cctgcgccag catcgcatcg gcaaccttca caaaaccggc aatgttcgcg
     1201 ccctgcacgt agttggtttg ctcaccttca ccaccatgct caacacaggc atggtggata
     1261 tccagcatga tgtgatgcaa acgtgcgtca actttctcgg ctttccagcc caggcgcgca
     1321 gcgttttgcg ccatttcaag gcctgacgtt gctacaccgc cagcattagc cgctttaccc
     1381 ggtgcaaaca gtacgcctgc ctgctggaac agttcagtcg cttcgatggt ggtcggcata
     1441 tttgcccctt cggcgacggc tttaacgcca ttggcgataa gctgatgcgc ggcgtcaaca
     1501 tccagttcgt tctgggtggc gcaaggcagg gcgatatcaa ccggtacaga ccacggctgt
     1561 tggcgttcga gatagaccag accaaattct ttggcataat ctgccactcg accatcgcgg
     1621 ctggctttga tttcgataag acgtgccagt ttctctttcg tgaatccgct ttcatcaact
     1681 acagtgccgc tggagtctga cgcagtgatc acacgagcac caaattccat cgctttttcg
     1741 atagcgtact gggcgacgtt gccggagcca gaaacggaaa cgcgcatccc ttcaaaaccc
     1801 ataccgtggc gttttagcat tgcttctgtg aaataaacca gaccgtagcc ggtagcttcc
     1861 gggcgaataa gactgccgcc aaatgaaagg cccttaccgg tgaagacgca ggcggtattg
     1921 ttggagagct ttttcatcat ccccgccata aagccgactt cacgaccacc aaccccgata
     1981 tcacctgccg gaacgtcggt atccgcgccc aggtggcgat acagttcagt catcagcgcc
     2041 tggcaaaaac gcatcacttc accttcgctt tttcctttcg gatcgaaatc gctgccgcct
     2101 ttaccaccgc ccatcggcag ggtagtcagg gcatttttga aggtttgttc gaagccgagg
     2161 aatttaagaa tggagaggtt aactgacgga tggaagcgca taccgccttt gtacgggccg
     2221 atggcagagc tgaactgcac acgccatgca cggttgacct gtacctgatt gcgatcatca
     2281 acccatacca cgcgaaactg gatcacgcgc tccggttcaa ccagacgctc cagtaatgac
     2341 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
     2401 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
     2461 tccagagaat atgtctgatc catagatata aaacccttat atattaatac gattgtgctt
     2521 atgttgttta tgtgatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
     2581 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
     2641 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
     2701 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
     2761 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
     2821 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggataaa
     2881 aattattctg aaggcgcggt aattaaagcc gagggaatac tgctgcaatg ccagcgggat
     2941 gacaaaaccc ttagcactaa tccgctggtc tggcggcgcg taaagccata aacgcctcta
     3001 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
     3061 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
     3121 gaagatggat aatccggccc gaaacttctc gctgatggct ggtaacatac tcacccacag
     3181 ttgcttcgat gcccagttct tcgcgtaact cacgcaccag cgcctgccgc tggctttcat
     3241 ccggctcgac tttaccaccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
     3301 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     3361 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     3421 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     3481 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     3541 ttaacattag tgggatttgc catcggcgtg ctggcactgc cttttctggc gctgggctgg
     3601 tatctggcgg cgcttgtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     3661 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     3721 cttttttacg cgctggtacc ttttgggttt atcctcgccg caccgcagca aaacgccctg
     3781 gcaggcggct ggctgctgtt tgcgtttatc ggcacaggca gcagttttct cgcttttgct
     3841 gcgctggcgg cgaaacatca gattgataat cccggttatg cgcataaatc gttttattat
     3901 ctgggcgggt taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     3961 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     4021 acgcgggtgt ggagtgggta tctgacgctg aagtcgcacc agcgtcagta attgcaatgt
     4081 tatttgctac tgtccgggcc gcgttcaccg gttgctaccg gattttttgg atcgctgctc
     4141 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     4201 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgctctggt
     4261 ttgatattcc acgctttcca catagcggta atatcatcgg cgctgcgcat ggtgccatcc
     4321 gggttatgaa aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     4381 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     4441 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     4501 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     4561 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     4621 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     4681 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     4741 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     4801 tcgttggtat cgatgtagtc agcaccggga atatggctga taaggtaaag cttaggagcg
     4861 ccccaggccg cttcaatgac tttccagtca ccggcaggtt tcgccgtaac ctctttacct
     4921 tgttgcaggt cgtgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     4981 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     5041 tgcagtcgcg ttttgacggc gtcgacatct ttgtcattac cgtacagcgc caccggggca
     5101 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcg
     5161 agccagctgg cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     5221 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgt
     5281 tgaagctggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     5341 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     5401 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     5461 agtttgttgt aattttctga ccactgctcc atatccagaa cagaactatc agcaggaaca
     5521 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gttcgcgaac ttcgctgaac
     5581 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     5641 agtaacgctt ttggctgagc gagaagggcg cgcagcaggg ccacccgcgc tcgctgaccg
     5701 cctgataatg tcgcagggtc ctggtgataa gtttcagcca gtcccgctcg atcaagcgcg
     5761 tctttcacgg cattgcgcct ggcagtcccc ttgagcgtcg acggtagcgc cagcagtaaa
     5821 ttttgcccga cgctgaactg gtcaaataac agtgcatcct ggaaaagaat gccaatctga
     5881 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     5941 gaaaactgtc cagccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     6001 gacggcccca ttaacgtgac aatgtcacct ttatccaccg taaagttaac gtttgtcagc
     6061 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggagtcctt
     6121 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     6181 atagcagttg ccataaagcc tgggcggcga aaataccatt gctgccgccg ctgcttaatg
     6241 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     6301 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcgtaggg cgtagcataa
     6361 gcggacattt cacgtaaaag aagattttgg cccgcgacca gctcagtgtt tgcgcaatca
     6421 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     6481 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     6541 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagagtg ggcaaccaga
     6601 gccacaactg gcgacgctgt ggcccccatt ccagccacag cagtaacagg agcaaagcga
     6661 tgaatgtcgc caccagcccc attgtcaggc tgttgatgag tgcttcacta ttgatcgtcg
     6721 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     6781 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     6841 gaatagtgcg ccgccagctg cgccatagca gatagctcag caaaacgtag gcggcgagta
     6901 acagcatcag cagcagactg gcgagtgcgc ctttcgtttg ttgatccgcg tcgccctgag
     6961 ttaaccactg ccagctaatt actgccagcg tcggtggatt acctggccca agaataatcg
     7021 ccacatccac gaccgaaagc gaccaggcaa caatcgccaa catcgccatt gccagtgcag
     7081 gtgcgacgga gggcaagagc agccagttca ggcattgcca gcggctgtag cccagcgaat
     7141 ccagcacaat gacctgctgc aacagccgtt tttcgctcaa tactgccgct aagatccaca
     7201 gcagaaatgc gctttctttc actgcaaggg tgaggcccag cccaataccc aatttgtcca
     7261 ttagcggagt gaaatacggg aaatagtcat aaagcagccc tccgtcagca aagagcagaa
     7321 gggcgctggt ggcaaaagcc acatggggaa tggcgagcag ccacggcaga cgggcacaca
     7381 tacgctgcca tttcggccaa ggccacagcg ccacaatcac cagcagggca atcaacaacg
     7441 ccccgaccgc cgcgatggtt gtcgacacca gcgttgccag taatgcctgc ggtaactgtg
     7501 gatcggcaaa taacgcctgc cagtgtgtca acgacaaagc aggcgatatc agcgtgagag
     7561 ctgccgggat cagtggtgca taaatcaccg ccaccatcgc ccacagcaaa aagattaatg
     7621 cgtaccgtaa cggtgtagcc attcttgttc cagcgcattt acccaacctg cgtgcggttc
     7681 agccagtacc ggcggcagat cttgcggcat tcttgattgc aatgtttcgc gctgcccatc
     7741 aggcagtttt tgcggatcga gaacagaagg atcgccccag acagcgggat ccgctttacg
     7801 cagttgcgca ttgggtgaga gcaggaagtt ggcaaccacc ttcgccgcag cactggcatt
     7861 tgcgttagca ggaatggtga cgaaatgcac gttgccaatc atcccctggt taaaaccaaa
     7921 actgtaactg cttgcaggca aatcgccgct ggcgattttt tgctgggcat gagcagggtt
     7981 aaaggtcagc gacaggcgca atgtgccggc tttcagcaga gcatccatcc gcgcgggcga
     8041 aggcgggaaa tcctttcctt tgcgccataa atagggatgt agcgcgtcaa gatattgcca
     8101 caagggcgca gtgacgcggg cgaaagtggc tttatccggg gcaactttta acgcggcggg
     8161 atcgggcgtc agcataatca gcaactgttc aagaaacgcc gtgccggtaa agtccggtgg
     8221 gcgcggatag gtaaccgtgc cgggattggc tttagcaaac tccagtaagg cttgcggcgt
     8281 ttgtggtggc tgtggcgtaa catcgcggcg ggcgataaac gtcagttgtg cgccgcccca
     8341 gggcgattca gccccttctg taggcactga aaaatcttcc cgcaccggca gctgtgtgtc
     8401 gacatagcgc cagttgggca gagtctccgc ccagcccgtt tgtaataaat tggcctcttt
     8461 taaggtgcgg aagttttcgc cgttcaccca gagcagatcc accgagccgc ccgttttacg
     8521 tccggcagca gcttcggtct gaatgcgctt caccgcgtct gcggcatccg ccagacgaac
     8581 aatcttcagg tttatagcgt aatgggtttt catctcgccg ctaacccagt cgagatagcg
     8641 gttaattgcg gtatcgccgc cccaggcgtt aaaccagacg gtttgccctt tggcctcatt
     8701 tttaatttcc tgccagtcac tggcatgtgt tgccagagaa aacagcgata acaatcccag
     8761 caaccaccca caatggcgca tcttctctcc ttaagaacgt ttttccggaa tacggcacag
     8821 cccctgagcg ggaatgtcca tggcagaatt aaaacgagcc gacaggtttt gcaggccgat
     8881 caatgccgtc agttcggtga gcgcctgagc gtcaaaatgc gcagccagtc gggtgcgcag
     8941 ggcatcatcg accgttggcg gcgttacgct tgcggcttcg gcgtactcca gcgccagccg
     9001 ttcttcatcg ctgaagagcg ggctttgccg ccagtcagcc actgccagca gtttatcggt
     9061 gctgccggta cgctcggcga ctttcatact ggtgatatcc acgcaaaact cacacaggca
     9121 catttgcgca atgcgcgcgc tgacaagcga tcgcactacc ggatcgagcg gtgagcgttt
     9181 gcgctccagc cacccaacaa acattgacac caaataaaag ataaacggga tccgccccca
     9241 ccagcgaatc gggcttagca cctcgccgta atggcgacga tgcagccacg cctgcggacg
     9301 taaaatgaaa ggaattttgc taagcggcgg taaacccatc aggcattcct cttctggcgc
     9361 tcaagccata ttttcttcgc cataaaaatc accacggtca gggcaaacag aataaacagt
     9421 ccggtgacaa accagaaagt accgcctgtt aacatgctgc ccgcccagga ataaacaata
     9481 gtcgccggta attgaccaag cccggtggcg ataaaaaacg agcgaaaacg tattgaagtc
     9541 agaccggcag catagctgat tggatcgaaa gggacaaaag gcaataaccg acagaccagg
     9601 atggtgtgtt tgccgtagcg agtgaaaaag ccgtccatac tgtcaagcac ggttttgccg
     9661 gttaattttt ccaccacttc gcggcccatc actctggcga taaaaaagca cagcgccgcg
     9721 ccggccatcg aactggtcca cgacagcaag ccgccccaga acgcgccaaa cagcgacgca
     9781 ttggcaaagg tgatcaaaaa cgcaggtagc ggtgcagcaa tggcctgtaa aatcatcaac
     9841 aagaacgaga caactgccgc cagtgcgccg taagactgaa taaagcgttc tataccctgt
     9901 tggtccacgg cggcaaacgc cgcaacgctg cgattgataa actcatggac gccgggaagc
     9961 agcgcccatg cgagcagcat agcgaacaac aggatgatga gggttatacg gtagtaccag
    10021 attttacgcg attgtatctt catcatcctt tatcctggct ttattcggtg attttaaagg
    10081 taaccgttgc cagcgtgtta tccgccggga gaactgaggc attgcctttg aatttcactt
    10141 cgccgcgttt ttccaccgca ccataagtgt atgttgcatt gctgacgatg ccgaccgggc
    10201 agctatccag acacaccagg caacccgttt tcttctcttc tgctgccgtt aaattaccgc
    10261 caaagcgcat gtccagtttc ttgccattac tgtcaacaat cacttcatcg aaggaatacg
    10321 cttttgccgc cccttgccag ttgaccgaaa tatccagttt gctgcctgtg acatgagtcg
    10381 tttctttatt atccatcgtc atgttttcgc ccggcgtacc acctgcctct ttcagggctt
    10441 cataaaatgc tttcggggtc gcataagcca taaacagcga tttatgtccg ttggagccat
    10501 ctttaaacac aataccgtga cgagtgtcgt cggtgaggaa acgaccatta atttgcacca
    10561 gcatggttac cgtatggtta ttggcatcga cgcgcagcgg ttggctgttc gaaagtccgt
    10621 cgtattccac ttttgccgcc gcattctctt tttgatcgca gccgctaagc ccggcaacag
    10681 ccagtaagca gagtgcagcc agtccttttt tccatgacac tgaataatgt tgcgacatcg
    10741 ttctatcctt cattttttgg gtgagtaagt gggctgcggc gcgaagcaga cagatccaca
    10801 tgtttgtgtc gggcgtagag ttttgcgagc tggacgagaa taaacagcgc caggcccgcc
    10861 agacagagtt gtaaaataaa gcgcagcgta atgccttcat tggcgagatc gcttgccatc
    10921 acggtataaa taacaatacc gagcagggtc gtgagtgccg aaataagggt ataaggccag
    10981 aaggcgatgg tggttaatcc gtaagcgtaa ttttgaatat tgtaaggaaa caacgggatt
    11041 aagcgggtca gaataagaaa atcaataccg ttacgcgcaa tgcctttttc aatggcctgg
    11101 aaggtatggc tatggcccac gtatttcagc agtaaatcac gtcccagcca gcgcgccagc
    11161 aggaatgagc acgaggaggc cagcgttgcg gcaattaatg agagtagtgt ccctaagagc
    11221 gggccaaaaa ctattcctcc agcgatcacc agtatgcttc ctggtaacag aaagagggtg
    11281 gcaatgatga ataacagaat atagaggcta taaccgaaca atccgctctg gcgaatgagt
    11341 gtctgtaagt ggggtaaatc ggtgagcaga tcgaataaac cgaaagcgtg gatagcgtaa
    11401 atgaccagcg caaaaataag acaggcaaac agaaagttac gctcagcgtt catcgtggag
    11461 tttcctatat caaacttgat aatagagaag tggtcagaca actaaaaacg cagtgacggt
    11521 ataacaaagg acggcaggca acaaatcatt actgacagtt tgttgatttt tctcaaggtt
    11581 aattctcctg acccagtttg agccaggaga gctgctaaat tagcggcgga aggtcgccca
    11641 gacgggggcg tgatcggacg gtttttccat gctgcgaatt tcatagtcga tgccggtttc
    11701 tacgcaacat tctgccagcg gttggctggc gagcagcagg tcgatgcgca gaccacggtt
    11761 atcgtcaaaa ccttttgagc ggtaatcaaa ccatgagaaa cgatctgctg tttgcggatt
    11821 agcatggcgg aaggtatcga ccaaccccca gctcatcagc ctgtccatcc attcgcgctc
    11881 ttccggcagg aaagagcatt taccggtacg cagccagcgc ttacggtttt cttcgccaat
    11941 gccgatatcc agatctgtag ggctgatatt catatcgccc ataatcagta ccggattatc
    12001 acgtttgagt tcggtttcca ggtagttttg cagattctga taaaactgcg cttttgccgg
    12061 gaattttatc ggatggtcgc ggctttcacc ctgcgggaag taaccgttga tcacggtgac
    12121 attacccagc agtgagggga tttccgccat aataatccgc cgctgcgcct cttcgtcgtc
    12181 accgggaaag ccgcgacgca cggcaatcgg cgtctctttg gtcagcagcg ccacgccata
    12241 atggcctttc tgcccgtgat aaaacacgtt gtagccgagc ttcgccacct cttcgagcgg
    12301 aaacatatcg tcatgaactt ttgtctcctg caggccaatc acatccggtt ggtgcttttc
    12361 gacgatggct tcaagctggt gaggtctggc gcgcaggccg ttgatattaa aagagacaaa
    12421 tttcatagtc gctgccattt attcagataa agagtgcgtg gatggtagca gaatttcgcg
    12481 ttgcttaccg cctgttgtta agtgatttga tcgatggttg ttaatgatgt caacgatggc
    12541 gcaaaaaatg cccgcttttg gtgcgcgctg cgtcagaatg gcgcagtaat ttccagtaaa
    12601 tttcgtcaat gatcacaatt ccagaattat attttacttc tgcataacat tgcgtttttt
    12661 attcttttta ttcacccaca tcgcaaacgt attcacttta tatgcacttt aaatgcatat
    12721 ggtttggtta taactccttg atttccagtt agcctccgcc gtttatgcac ttttatcact
    12781 ggctggcacg aaccctgcaa tctacattta cagcgcaaac attacttatt gttaacatat
    12841 aaataacgaa ttatttactg tagaggtcgc tatgtctcag ccaattacgc gtgaaaactt
    12901 tgatgaatgg atgatacctg tttacgctcc ggcacccttt ataccggtac gtggcgaagg
    12961 ttcgcgcttg tgggatcagc tggggaaaga gtatatcgac ttcgcgggtg gcattgcggt
    13021 gaacgcgctg ggccatgcgc atccggaact gcgtgaagcg ctgaacgaac aggcgagtaa
    13081 gttctggcat accggcaacg gttacaccaa cgagccggta ctgcgactgg cgaaaaaatt
    13141 gatcgacgcc acgtttgccg atcgcgtctt cttttgtaac tccggtgcgg aagccaacga
    13201 agcggcgcta aaactggcgc gtaaattcgc tcacgaccgc tacggcagcc ataagagcgg
    13261 catcgtggcg ttcaaaaatg cgtttcatgg tcgcacgctg tttactgtca gtgcgggtgg
    13321 gcagccagcc tattcacagg attttgcgcc actgccggcg gatattcgtc atgctgcata
    13381 taacgatatt aactctgcca gcgcgctgat tgacgactct acctgtgcgg tgattgtcga
    13441 acccatccag ggggaaggcg gtgtggtgcc agccagcaac gcgtttttac aaggtctgcg
    13501 tgaattgtgt aaccgccaca atgcgctgtt gatttttgat gaagtacaaa ccggcgtcgg
    13561 gcgcaccggg gaactgtatg cctatatgca ctacggcgtg acgcctgatc tgttaactac
    13621 cgccaaagcg ctgggcggcg gtttcccggt cggtgcgttg ttggcaaccg aagagtgcgc
    13681 ccgcgtgatg accgttggca ctcatggcac cacctatggc ggtaatccgc tggcctcggc
    13741 ggtggcaggc aaagtgctgg agctcatcaa cacaccagag atgcttaatg gcgttaaaca
    13801 gcgtcacgac tggtttgttg agcgtcttaa tactattaat caccgctatg gtttgttcag
    13861 tgaagttcgc ggcttaggtt tgctgattgg ctgtgtactg aatgccgatt acgccgggca
    13921 agcgaaacag atctctcagg aagcggcgaa agcaggcgtg atggtactga ttgcgggtgg
    13981 caacgtggtg cgttttgcgc ctgcgctcaa tgtcagcgaa gaagaggtga cgaccggact
    14041 ggatcgcttt gcagctgctt gcgaacactt tgttagccga ggttcatcat gatggtcatc
    14101 cgtcccgttg agcgatcaga tgtctcggcg ctgatgcagc ttgccagcaa aacgggcggc
    14161 ggcctgacgt cgcttcccgc caatgaagcc acgctttcgg cgcgtatcga aagggcaatc
    14221 aaaacctggc aaggcgaact gcccaaaagt gagcagggct atgtgttcgt gctggaagat
    14281 agcgagacag gcaccgtggc ggggatttgt gccattgagg tggcggttgg gctgaacgat
    14341 ccctggtaca actatcgcgt cggcacgttg gttcacgcct caaaagagct gaatgtctat
    14401 aacgcattgc cgacgctgtt tctcagtaac gatcacaccg gcagcagcga gctgtgcacg
    14461 ctgtttctcg acccggactg gcgcaaagag ggcaacggct atttgctgtc gaaatcgcgc
    14521 tttatgttta tggcggcttt tcgcgacaag tttaatgaca aagtggttgc tgaaatgcgc
    14581 ggggtgattg acgaacacgg ctattcaccg ttctggcaaa gcctcggtaa acgcttcttt
    14641 tcgatggatt ttagccgcgc cgattttctc tgcggcaccg ggcaaaaggc atttattgca
    14701 gaactgatgc cgaaacatcc gatctatacc cactttttat cccaggaagc ccaggacgtc
    14761 atcggtcagg tacatccgca aaccgcgcct gcccgcgcgg tgctggagaa agaaggtttt
    14821 cgttaccgca actatatcga catcttcgac ggcgggccaa cgcttgagtg tgacatcgac
    14881 cgcgtgcgcg ccattcgtaa aagtcggctg gtggaagtgg cagaagggca gcctgcgcag
    14941 ggcgattttc cggcctgcct ggtcgccaat gaaaattatc accatttccg cgtggtgctg
    15001 gcgcgtaccg atccggcaac cgagcgtttg attttaaccg ccgcacaact ggatgccctc
    15061 aaatgccacg caggggatcg cgttcgtctg gtgcgcctgt gcgcagagga gaaaacagca
    15121 tgactttatg gattaacggt gactggataa cgggccaggg cgcatcgcgt gtgaagcgta
    15181 atccggtatc gggcgaggtg ttatggcaag gcaatgatgc cgatgccgct caggtcgaac
    15241 aggcttgtcg ggcagcccgt gcggcgtttc cgcgctgggc gcggctctca ttggctgaac
    15301 gtcaggtcgt tgtcgaacgc tttgccggat tgctggaaag gaataaaggc gaattaaccg
    15361 cgattattgc cagagaaacg ggtaagccgc gctgggaagc ggcaaccgaa gtgacggcga
    15421 tgatcaataa aatcgcgata tcaattaagg cgtatcacgt tcgtaccggc gagcagcgta
    15481 gtgaaatgcc ggacggtgcg gcgagcctgc gacatcgccc gcacggcgtg ctggcggtgt
    15541 ttgggccgta taatttccct ggtcatttgc cgaacggaca tatcgttccg gcattgctgg
    15601 caggtaacac cattatcttt aaacccagcg aactgacacc gtggagtggc gaagcggtaa
    15661 tgcgtttatg gcagcaggct ggcttgccgc cgggcgtact gaacctggtg cagggcgggc
    15721 gtgaaacggg tcaggcgctg agtgcgctgg aggatctcga cggtttgctg tttaccggta
    15781 gcgccaatac cggctaccag ctgcatcgcc agctctccgg tcagccggag aaaattctcg
    15841 cccttgagat gggcggtaat aatccgctaa ttatcgatga ggtggcggat atcgacgcgg
    15901 ctgtccatct gaccattcag tcggcgtttg tcacagccgg gcaacgctgc acctgcgccc
    15961 gccgtttatt gctcaaaagc ggagcgcagg gcgatgcgtt tcttgctcgt ctggttgccg
    16021 tcagccagcg attaacgccg ggcaactggg atgacgaacc gcagccgttt attggcgggc
    16081 tgatttctga acaggccgca cagcaggtgg ttactgcctg gcagcaactg gaagcgatgg
    16141 gcggacgaac cctgcttgcg ccgcgcttat tacaatcaga gacatcgttg ctgacgccgg
    16201 ggatcattga aatgacaggc gttgctggcg taccagatga agaggtgttc ggaccgttat
    16261 tgcgcgtctg gcgttatgat tctttcgagg aagcgattct aatggcgaat aacactcgct
    16321 tcggactctc ttgcggtctg gtttcccccg agcgggaaaa attcgatcaa ctgttgctgg
    16381 aggcgcgggc ggggattgtt aactggaaca aaccgcttac tggtgctgcc agtaccgcgc
    16441 cattcggcgg cattggtgca tccggtaacc atcgccccag cgcctggtat gccgcagatt
    16501 actgcgcatg gcctatggcg agcctggagt cggactcgtt aactttgccc gcaacgctta
    16561 accccgggct ggatttttcc gatgaggtgg tgcgatgaac gcctgggaag ttaatttcga
    16621 cgggctggta gggctgacgc atcattacgc tggcctgtcg tttggtaatg aagcctctac
    16681 ccgtcaccgt tttcaggtgt ctaacccgcg acaggcggca aaacagggct tactgaaaat
    16741 gaaagccctt gccgatgcgg gattccccca ggccgtgatc ccgccgcacg agcgtccgtt
    16801 tattccggtg ctgcgtcagt tgggattcag tggtagcgat gagcaggtac tggaaaaagt
    16861 tgcacgccag gcaccgcact ggctttccag cgtcagttcc gcttcgccaa tgtgggtagc
    16921 caatgcggca acgatcgcgc catctgccga tacgctggat ggcaaagtgc atctcaccgt
    16981 tgccaacctg aacaataaat ttcaccgctc gctggaagcg cacgtcaccg aatcactttt
    17041 aaaagcgatt tttaacgacg aagagaaatt tagcgttcat tcggcgttgc cgcaggtagc
    17101 gttgctcggt gatgaggggg cggcaaacca caatcgtctc ggcggtcatt acggtgagcc
    17161 gggtatgcaa ctttttgtct acgggcgaga agaaggcaat gatacccggc cttcccgtta
    17221 tccggcgcga cagactcgcg aagccagcga ggcggtggca aggctgaatc aggtgaatcc
    17281 ccaacaggtg attttcgccc agcaaaaccc ggacgttatc gaccagggcg tttttcataa
    17341 tgacgtgatt gccgtgagta accgccaggt gctgttttgc caccaacagg cgttcgctcg
    17401 ccagtcacag ttactggcaa acctgcgtgc gcgggtgaac ggttttatgg cgatagaagt
    17461 tccggcaact caggtttccg tgtcggatgc ggtgtctacc tatctgttta acagccaact
    17521 gttgagccgc gatgatggtt ctatgatgtt ggtgctgcct caggagtgtc gggaacacgc
    17581 cggagtatgg ggttatctca atgaactcct tgccgctgac aacccgatta gcgaactaaa
    17641 agtctttgat ttacgtgaaa gcatggcgaa tggcggcggc ccggcgtgcc tgcggttgcg
    17701 ggtggtattg acagaagaag aacgccgggc ggtgaatccg gcggtgatga tgaacgatac
    17761 gctgtttaat gcgctcaatg actgggtgga tcgttactac cgcgatcgcc ttactgctgc
    17821 cgatctggcc gacccgcaat tgctgcgcga agggcgggaa gcactggatg tattgagcca
    17881 attactgaat ctcggttcgg tttatccgtt ccagcgcgag ggagggggca atggataatt
    17941 ttcttgctct gaccttaacg ggtaaaaaac cggttatcac cgagcgagaa atcaacggcg
    18001 ttcgctggcg ctggctgggc gatggtgtgc tggaactgac gccattaacg ccaccgcaag
    18061 gcgcactggt gatttcagcg ggaatacacg gtaatgagac ggcacctgtg gagatgctgg
    18121 acgcgttgct tggcgcgata tctcacggcg agatcccgtt acgttggcgg ttgctggtga
    18181 tcctcgggaa tcctcctgcg ctgaagcaag ggaaacgtta ttgccatagc gatatgaatc
    18241 gaatgtttgg cggtcgttgg cagctatttg ctgaaagcgg agaaacctgt cgggcgcgcg
    18301 aactggaaca gtgcctggaa gatttttatg accagggcaa agaatctgtg cgctggcacc
    18361 ttgatctaca taccgcaatt cgtggctcct tgcatccgca gttcggtgta ttaccgcaac
    18421 gcgacattcc ctgggacgag aaatttctga cgtggctggg tgcggcgggg ctggaggcgc
    18481 tggtgttcca tcaggaacct ggtggtacgt ttacccattt cagcgccaga cattttggcg
    18541 cgctggcctg tacgctggaa cttggcaaag cgttgccctt tgggcaaaac gatcttcgcc
    18601 agtttgcagt aactgccagc gcaattgctg cgctgctatc tggtgagagt gtcggtatcg
    18661 tgagaacacc gccgctccgt tatcgggtgg tttcgcaaat tactcgccac tcgccgtcct
    18721 tcgaaatgca tatggcaagt gacacgctga attttatgcc gtttgagaaa ggaacattgc
    18781 tggcgcagga cggagaggaa cgttttaccg taacccatga tgtagagtat gtgttattcc
    18841 ctaatccgtt ggtagcgttg ggattacgcg cgggattaat gctcgaaaaa ataagctaat
    18901 acttacccgc agaaatcact ctgcgggtat taagttgctt tcttataaat taatacag
//
LOCUS       DAZFWL020000088        18747 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFWL020000088 DAZFWL020000000
VERSION     DAZFWL020000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.088"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            345..1244
                     /gene="yegS"
                     /locus_tag="ACHKNP_RS16265"
                     /old_locus_tag="pgaptmp_003253"
     CDS             345..1244
                     /gene="yegS"
                     /locus_tag="ACHKNP_RS16265"
                     /old_locus_tag="pgaptmp_003253"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HGU4705370.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1326..2099)
                     /locus_tag="ACHKNP_RS16270"
                     /old_locus_tag="pgaptmp_003254"
     CDS             complement(1326..2099)
                     /locus_tag="ACHKNP_RS16270"
                     /old_locus_tag="pgaptmp_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4705371.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2205..3245)
                     /gene="gatD"
                     /locus_tag="ACHKNP_RS16275"
                     /old_locus_tag="pgaptmp_003255"
                     /pseudo
     CDS             complement(2205..3245)
                     /gene="gatD"
                     /locus_tag="ACHKNP_RS16275"
                     /old_locus_tag="pgaptmp_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
     gene            complement(3293..4648)
                     /locus_tag="ACHKNP_RS16280"
                     /old_locus_tag="pgaptmp_003256"
     CDS             complement(3293..4648)
                     /locus_tag="ACHKNP_RS16280"
                     /old_locus_tag="pgaptmp_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGU4705372.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4652..4936)
                     /gene="gatB"
                     /locus_tag="ACHKNP_RS16285"
                     /old_locus_tag="pgaptmp_003257"
     CDS             complement(4652..4936)
                     /gene="gatB"
                     /locus_tag="ACHKNP_RS16285"
                     /old_locus_tag="pgaptmp_003257"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HGU4705373.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(4967..5419)
                     /gene="gatA"
                     /locus_tag="ACHKNP_RS16290"
                     /old_locus_tag="pgaptmp_003258"
     CDS             complement(4967..5419)
                     /gene="gatA"
                     /locus_tag="ACHKNP_RS16290"
                     /old_locus_tag="pgaptmp_003258"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HGU4705374.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5429..6691)
                     /gene="gatZ"
                     /locus_tag="ACHKNP_RS16295"
                     /old_locus_tag="pgaptmp_003259"
     CDS             complement(5429..6691)
                     /gene="gatZ"
                     /locus_tag="ACHKNP_RS16295"
                     /old_locus_tag="pgaptmp_003259"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HGU4705375.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6720..7574)
                     /gene="gatY"
                     /locus_tag="ACHKNP_RS16300"
                     /old_locus_tag="pgaptmp_003260"
     CDS             complement(6720..7574)
                     /gene="gatY"
                     /locus_tag="ACHKNP_RS16300"
                     /old_locus_tag="pgaptmp_003260"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HGU4705376.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7884..8936)
                     /gene="fbaB"
                     /locus_tag="ACHKNP_RS16305"
                     /old_locus_tag="pgaptmp_003261"
     CDS             complement(7884..8936)
                     /gene="fbaB"
                     /locus_tag="ACHKNP_RS16305"
                     /old_locus_tag="pgaptmp_003261"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HGU4705377.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9193..10470
                     /locus_tag="ACHKNP_RS16310"
                     /old_locus_tag="pgaptmp_003262"
     CDS             9193..10470
                     /locus_tag="ACHKNP_RS16310"
                     /old_locus_tag="pgaptmp_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HGU4705378.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10467..11471
                     /locus_tag="ACHKNP_RS16315"
                     /old_locus_tag="pgaptmp_003263"
     CDS             10467..11471
                     /locus_tag="ACHKNP_RS16315"
                     /old_locus_tag="pgaptmp_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGU4705379.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            11468..12433
                     /locus_tag="ACHKNP_RS16320"
                     /old_locus_tag="pgaptmp_003264"
     CDS             11468..12433
                     /locus_tag="ACHKNP_RS16320"
                     /old_locus_tag="pgaptmp_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGU4705380.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12407..13153)
                     /locus_tag="ACHKNP_RS16325"
                     /old_locus_tag="pgaptmp_003265"
     CDS             complement(12407..13153)
                     /locus_tag="ACHKNP_RS16325"
                     /old_locus_tag="pgaptmp_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4705381.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13205..14023)
                     /locus_tag="ACHKNP_RS16330"
                     /old_locus_tag="pgaptmp_003266"
     CDS             complement(13205..14023)
                     /locus_tag="ACHKNP_RS16330"
                     /old_locus_tag="pgaptmp_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGU4705382.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(14088..14888)
                     /gene="thiD"
                     /locus_tag="ACHKNP_RS16335"
                     /old_locus_tag="pgaptmp_003267"
     CDS             complement(14088..14888)
                     /gene="thiD"
                     /locus_tag="ACHKNP_RS16335"
                     /old_locus_tag="pgaptmp_003267"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGU4705383.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14885..15673)
                     /gene="thiM"
                     /locus_tag="ACHKNP_RS16340"
                     /old_locus_tag="pgaptmp_003268"
     CDS             complement(14885..15673)
                     /gene="thiM"
                     /locus_tag="ACHKNP_RS16340"
                     /old_locus_tag="pgaptmp_003268"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGU4705384.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15708..15804)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15896..16168)
                     /gene="rcnR"
                     /locus_tag="ACHKNP_RS16345"
                     /old_locus_tag="pgaptmp_003269"
     CDS             complement(15896..16168)
                     /gene="rcnR"
                     /locus_tag="ACHKNP_RS16345"
                     /old_locus_tag="pgaptmp_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HGU4705385.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16289..17113
                     /gene="rcnA"
                     /locus_tag="ACHKNP_RS16350"
                     /old_locus_tag="pgaptmp_003270"
     CDS             16289..17113
                     /gene="rcnA"
                     /locus_tag="ACHKNP_RS16350"
                     /old_locus_tag="pgaptmp_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HGU4705386.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVVSKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWHGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            17332..17670
                     /gene="rcnB"
                     /locus_tag="ACHKNP_RS16355"
                     /old_locus_tag="pgaptmp_003271"
     CDS             17332..17670
                     /gene="rcnB"
                     /locus_tag="ACHKNP_RS16355"
                     /old_locus_tag="pgaptmp_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HGU4705387.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17752..>18747)
                     /locus_tag="ACHKNP_RS16360"
                     /old_locus_tag="pgaptmp_003272"
     CDS             complement(17752..>18747)
                     /locus_tag="ACHKNP_RS16360"
                     /old_locus_tag="pgaptmp_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HGU4705388.1"
                     /translation="VMPVSYAACYSELSVQHNLVVQGDFALTQTQMATYEHNFNDSSC
                     VSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTSFTSGYSVTVTPAA
                     SNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGGKSWDACVNSYRNA
                     LAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATINSKQGDIIL
                     RCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFILESNGSSVTLLNIT
                     NSSKRYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALINVKYD"
BASE COUNT         4637 a         4773 c         4506 g         4831 t
ORIGIN      
        1 attgaatagg attataataa atgttctgta caacatttcc tacataagta ggaattacgg
       61 acattgaggc ccttcagggt aactccatgg gggctttaat atatttggaa tagtatattt
      121 aagatgccac tgtttggttg aatattaggc atatgctctt ttttgaaatt tatcggtggc
      181 agccgttagt attcgctgtc cccattgcaa gctcctggtg gtaaccactg aatcctccat
      241 acttgaactg actttttatc ctccgacttt catcctgttc tgactccacc ttttgttttc
      301 tgctctacac tatctacaga ccaatcataa aggcacatac gatcatggca gaatttcccg
      361 ccagcttact gattcttaat ggcaaaagta ctgacaatct acccttgcgc gaagcaatta
      421 tgctgttgcg tgaggaagga atgacgatcc atgtgcgggt cacctgggag aaaggcgatg
      481 ccgcacgata tgtagaggag gcccggaagt tgggcgtcgc aacggtgatt gccggtggtg
      541 gcgatggcac cattaatgaa gtttctacgg cgttgattca gtgtgagggg gatgacatac
      601 ccgcgctggg aattttgcca ttaggaaccg ccaatgattt tgccaccagt gtagggattc
      661 ctgaggcact ggataaggcg ctgaaactgg caattgccgg taacgccatt gcgatagata
      721 tggcgcaggt caacaaacaa acctgtttta ttaatatggc gacaggcgga tttgggacgc
      781 gtattaccac agaaacgccg gaaaaattaa aagccgcgct gggtggcgtc tcttacatca
      841 ttcatggctt aatgcgcatg gatactctgc aaccggaccg ttgtgaaatc cgcggtgaaa
      901 actttcactg gcaaggtgac gccctggtca ttggtattgg taacgggcgt caggccggtg
      961 gcggtcagca attgtgtccg aacgcgttaa ttaacgatgg cttgctgcaa ctgcgcattt
     1021 ttaccggcga tgaaattctt ccggctctcg tatcaacctt aaaatctgac gaagataacc
     1081 cgaatattat cgaaggcgct tcgtcgtggt ttgatataca agccccacac gaaatcactt
     1141 ttaatcttga tggcgaaccg ttgagcggac aaaactttca tattgaaata cttccggcgg
     1201 cgttgcgttg tcgattacca ccagattgtc cattattgcg ttaatcaaac tattctctct
     1261 tgaaccaggg agaatccgaa atgccggatg cggtgtaaat accttatccg gcatacagaa
     1321 caatactaca ccgtaattaa ttttacccgt gtcttctgca atgccagttt atccacctct
     1381 gatacagcat catcggtaat aataatgtca attttttcga ttggcagtac ctgattaaaa
     1441 ccgcgacggt taaatttcga tgaatcgaga acggcaatca ctttattagc ggcggtgacc
     1501 atcgcaccgc taatcgaata accttcatta aaggtcgtaa taccattaac cgcatcaata
     1561 ccatcagcac cgacaaacat taaatcggca ttaatatcct gcagggaacg ttcagcaata
     1621 gatccatgca tagatcgcgt tttatggcgg accgtgccac cgcagacgac cagagtaatg
     1681 tctttatttt cggaaagggc aaacgcagcc gggagactgt tggtaatcac cgtgatattt
     1741 ttggcggtca ttaatccttc ggcgataagc attgtggtac ttccgctgtc gagaataacg
     1801 gtcatccctt catggatcat tttgactgcc gcctgcgcta ttctgttttt cggcgcgctg
     1861 gcgagctgaa atcgctcttt aaacccgact tcctgggtct cggtttcact attaccggac
     1921 attattttcg ccgcaccgcc atgaaagcgc gtaaccacgc ctttttgttc gagaaagcgc
     1981 aaatcggcac ggattgtcgc ttccgaggca gcaaatactc ccgccagatc ctgaacaagc
     2041 acggttccct gttcattcac taattggatg atcttattcc ttcgctcgaa tgaattcatc
     2101 gtcatttacc catattcaat tgtggctagt gtaaacgaag ggggaccatt ggtgaatgat
     2161 ctgttttgca caataccggg ccagcatcac gctggcccgc ggtttcaggg aatgagcaac
     2221 actttgcccg gcatagcatt acgagcgatg tcacgcaccg cctgggcgaa gctttcaaag
     2281 cttccacggt gagcgattaa tggctccagg cttaacttac gttctgtcag caaccggctc
     2341 gccgtttccc actcctgccc cggccaaggg ctggagtagt tcatccaact gccgataacc
     2401 gtcagctctt tacgcaatat tttgccaaac gttgccgatg ttaaatgcag atcctgatgc
     2461 aacgtgccca ccagcgccag ttgggcatga ggaccggcaa tctctaccgc cagttcgaca
     2521 gtttgcggta cgccagccgt ctcgaggata agctgattaa agcgcagtta gcgtaaaacg
     2581 ctctgcattt gcggcgcgct catttcgcta ctgttaaatg tttgcatcgc accgaaagat
     2641 tttgccagtg ccagtttttc tgaactaatg tcgatcgccg tcacactctt tgctcccagc
     2701 gcgacagcgc actgaatggc cagcaggcca atggttccgg caccaataat aataacgttt
     2761 ttattctcac aaccttgcgc taaatgaaaa gcatgcaggc caacggtaat cggttcaata
     2821 aaagccccat cctcaatagg catatcagta ggtagagcaa agacattttt ccgcttaacg
     2881 acaatatatt cggcaaatcc accatcacgc cgtgagccaa taaaatcata ttttgcgcac
     2941 tgggaataaa accctttcaa acactctgga caagtaaaac agggtaataa cggcacacag
     3001 gcaaccgcat cgccaggatg taaatcatca acaccggatc ccaccgcatc aatatagccg
     3061 ctaaattcat ggcctaacgt tattggataa taatgtgcac catttttaaa tatcctgggt
     3121 aaatcggaac cacataagcc cgagctggca atttttaccc gcacctcatc ctgatgttta
     3181 atttcaggaa tgacgctttc tgcaacgcgc acgataccat cagtatcatt caccactgat
     3241 ttcataaaaa ctcctgattg ttaaggggga taacctcccc ctcaggtaaa aattattctg
     3301 cgagaacgac tttctcttgt ttaataaagc cacgcgctct acgccaggtc atgaaaatac
     3361 cggtaagata aattgcgccg ataataatga atccgggaat attttgcggg gagaaaacct
     3421 gaatcagtaa ccaggtaatg ggagaaccgc cctgatccat tgaagccacc atacccccgg
     3481 cttttaacgc cccagcatta gccgccagtt gggtgtgcaa accaatagtt tgcgtcgcga
     3541 tccacagagt gatgctcatg atgatgacac ccgagattaa ggtgcggaac agatttccac
     3601 gatgcacggc gactgccatc gccacaaaga agccgatggt ggcaagatcg ccaaacggca
     3661 gcacctgatt acccggaaca caaacagcaa ttaaaatggt gagcgggata aaaatcaggc
     3721 ttgccgatac caccgccgta tgccccagca gtaatgcggg atccaggcca atcaggaact
     3781 cctgaccgcc gaacttcgcc tgtaaacgac tacgggcctg cttagcgatg ggcgttaaac
     3841 catccatgat gggtttaatc acccgtggca ttagcagcat cactgccgcc gtttttaccg
     3901 ccagctgcaa tacacctttg acatcgtaac ccgcgaggat gccgataatc agccccatca
     3961 caaaacccac ggtgacaggc tcgccaaatg gaccaaattt gcgctgaata tcgtcggcgc
     4021 taaatttaat tcggttaacg cctgggattt tctcgatgat agcatcgacc agcaccgcaa
     4081 tcggccccat atacgccgac gtaccgtgcg gaatagcaat accttccagc tcaaagaaat
     4141 ttcgggtatc gcgggcaaac cagtcgccga gcttataaac aaacgccgcg tgaattacca
     4201 cacctgccat ccctatcatc catgaaccgg ttgccaggtg cagcaacgcg ccggtgaagg
     4261 tcatatgcca gatattccag atatcaacat ttaccacccg cgtcatacgg gtcagtaaca
     4321 tcgccacgtt aaccaatatc gcaatcggaa tcgccaccag cgcaatttgc gacgcccagg
     4381 tcattggtga agagcccggc cagccgacat cgaccacatg cagattcagg tcgaaatttt
     4441 ccgccatcgc tttcgccgcc ggaccaatgg aatccagcat taagccaatc acaaggccaa
     4501 tgccaacaaa gccaatcccg atatgcagac ccgctttaaa gcaatcgcct gccttcatgc
     4561 ctaatatttt agaaaaaata atgatgacaa tcggaagcat caccgtaggg ccgaggtcga
     4621 gaatataacg catgacttct gaaaacatag gtcacccctg taagatagtc agaattttat
     4681 tttgtaatgc ttcgataccg acaccagaaa taaaaggcat gccgtgaact aacggaatat
     4741 cgccaaaact acgatccact ttggcagtgg tgcatatcaa atgcacacca tccatatagg
     4801 tttctatttc attaacccga cactggatta attcaacagg aatattatga ttctgacaca
     4861 actctttaat ttcttccgcc gccatcgtag aggtcgcaac cgcgcctccg caagcgacaa
     4921 taatcttgcg tttcataaat agtccttttt attgttacag agggatttat tcgtctgaat
     4981 ctaaaacata ctttgtgaag tattccttta actgagtttc aggaagagta attagtgtct
     5041 cgacgatatc gggctgttgt aacttgccaa ataaacagcg taaaagtttc aattgctgct
     5101 gcggattttc cacaatcaac gcaataacca acgacacctc cacgtcgtta tcatcatccg
     5161 cttgctgaaa atgaacttta tttgttggcc ttaacagata aatggctgac gacttagcat
     5221 gaatcgcctc acaatgcggt atagcaatgg cgtgctgctc aagcattatc ccggtaggga
     5281 attctgcttc tctggcaatt aatgcctgtg gccaggtatc ataaaccaca cctttcgcga
     5341 gcatctcatt accgatatgg gttaaaactt cgctacgatc gacaaaagaa attccgctac
     5401 gaacaaacag gttagtcata taccgtcctt attccgcaca gccgtagcga taggcgcgca
     5461 aaacatcata aattttatcc ataatcagct gatgcggtat tgctgataat tccccggact
     5521 gaatgcgttc aaattgtttg ggaagatact gactaatcat gcccagtggg atgtccacgc
     5581 cttcaagatt caccatcatc gtttcgacgc tatttttaat ccgactatgc ggccagtaat
     5641 aacgaatacg atccgacagg ctgtaacgaa tatccagtaa tgaatcgtta aaacccgtac
     5701 gataatattt tttccagtat tgcggttcgt cgagcatcac ttcttcaatt accgccaggc
     5761 aaccgctgcg attttcaggg gcgataagtt cctgctcaat ttgtgccagt gcaaatatcg
     5821 cctcgcgtaa agcaaaggtt aatgcgggac cgactttcaa tattgcaaag tgatcgcgga
     5881 ctaattccca ataagccgtc cgggtctggt aatcggtaga atgtgcttca taaaccattc
     5941 gggtgctttc tatccattgc gccagcggct gcgcttcctg cggctgataa tggataatat
     6001 tgctgtgatc aaattccacg cccggctgca ccacgatggc aatcacgcgt gttaacgcct
     6061 ctgtcagccc acgggcaata aaggcctttt gatgcgtacg taaagtattg gcggcatctt
     6121 caacatgggt gatgtgtact gactgaatgg cgctggcctc accgcccgga accggaactt
     6181 cggtgccaat gacatagctc agttgctcac gctggcaatc tgtcgccaca ctttccgcag
     6241 caaagcaaag cacagcagct cgttccgcaa ccgtttctgg tgctaacggt atgggatccc
     6301 ccgcgcagga cattgacgca tcaagatgaa ttttactgaa accggcacga acatattcct
     6361 ttaccagctc gacggatttt tccatcgccg catccgcatt ttcttgctgc cagcagtttg
     6421 gccccagatg atcgccgccg agaataatgc gttcgcgtgc aaacccaact ttatcggcaa
     6481 tcgtaaaaac aaattcgcga aagtctgccg gtgtcattcc ggtataaccg ccaaattgat
     6541 tgacctggtt tgacgttgct tcaatcagca ctttgcgcgt gctgttgcga tcaaatgcca
     6601 gcgccgcttc gataaccaac ggatgggcag aacagactga acatatgccg atatgttcac
     6661 cagctttatg ccgggcaatt aacgttttca tgttttttcc ttattaaatg gcaggtgcgt
     6721 tatgccctgc cctcgcagcc acaatcggca atcactttgc tcaccacatc gcgcattgcg
     6781 gatttagccg actgcaaata atcccgggga tcggtcgctt cagggtgctc ggtcaggtaa
     6841 ttttttaacg cctgcgagaa ggcatttttc agctccgttg caacgttgat tttgcatatc
     6901 cccagtttga tggtttgctg aatatcctta gtcgataacc ctgacgcgcc atgcagcacc
     6961 agcggtaagt tcacccactg gcgaatgttc tccagtcgag aaaaatcaag cgccggtgcg
     7021 ctggcataca tcccatgagc cgtgccgatg gcgaccgcca gggaatcaat tccggttgcc
     7081 tcggcaaatt cacgcgcctg agcggggttg gtataaaaag catcggcttc attgacttgc
     7141 acatcatctt cctggccgcc aagttgcccc agctccgctt cgacgctgac atcaaagcga
     7201 tggcaaaaat ccaccacctc tttgacccgt gaaatatttt gcgcaaaagg caaatgcgag
     7261 gcgtcaatca tgactgagcg cacgccagaa cgaaccttct gagcgatatc gtcaaatttc
     7321 gtgtgatggt cgagatgaat tgccagtgga tggtgatatt gcttcgccat cgcgctgacc
     7381 agcgccaaca gattttctgt accagcatga gtaaatgtgc caggcgttcc ggcgatgatg
     7441 accggcgcat gcaggttggc agcggtttcc accaccactt gcatcgtttc gagattgtga
     7501 atattgaatg ccggaaccgc ataaccgccg cgctgtgcgt tgttcagcat ctgctttgtc
     7561 gataccacgt acattttcat atcctgtcgt ttgttttcga tttcaaaata taatgaaatg
     7621 atttgttttt aaatgtcgag ataacgatca caaaagcgac aatgtgaaaa ttgttcgagg
     7681 agtaaaaggc aaaaaaaaac ggcctcccga tagggaagcc gtagcaaagt gcgcgtgttt
     7741 ttatgccgga tgcggtgtaa acatacgtgg cagtgctatg taggcatgat aagacgcttc
     7801 cgcgtcgcat caggcatagg ttgccggatg cagtgtaaac gccttatccg tcctacgggg
     7861 tggtgctgtt cgtatgaggc gtatcaggca atagtaattt tgctatcgag ataaacgtcc
     7921 tgcacggcat taatcagttt cacgccgtca gccatcgatt tcttgaacgc cttacgtcca
     7981 agaatcagcc ccattccgcc agcgcgtttg ttgataaccg cagtacgcac tgcatcgctg
     8041 aggtcagttt caccgcccgc agcaccgccg gagtttatca acccagcccg acccatatag
     8101 cagttagcta actgataacg caccagatca atcgggtttt cgctggtcaa tttgctgtaa
     8161 acacgatcgt cggtgtaacc gtaattaatt gctttatagc cgccgttatt ttccgccatt
     8221 ttttgtttga cgatatctgc accgatggtt gccgccagat ggtttgcctg accggtcagg
     8281 tcggcggaaa catggtaatc aacgccatct ttcttaaagg cggagttacg caaataggcc
     8341 cacagcactg tcaccatacc cagctcgtgc gcacgttcaa aagccgcaga aatttcttca
     8401 atctggcgac gtgactcttc cgagccaaaa tagatagtcg caccaaccgc aaccgcgccc
     8461 atgttgaacg cctgctccac gctggcatac agcgtttgat cgtaggtatt cgggtaactt
     8521 agcgtctcgt tgtgattaag tttgacgagg aatggaatgc gatgcgcata acgccgcgat
     8581 accgacgcca gcacgccgta agttgacgcc acacagttac agcccgcttc gatcgccagt
     8641 tcaacaatgt ttttcgggtc aaagtagagc gggttagcag caaatgaagc tccggcagag
     8701 tgctcaacgc cctggtcaac cggcagaata gaaagatatc ctgtgccagc cagacgcccg
     8761 gtgttgtaca acgtctgcat attacgtaac accgctggcg ggcgattatt gtcaatcatt
     8821 acgcggtcta cgtagtcatg tccggggaga taaagctggt cagaaggaat tgtcatacaa
     8881 cggtgctgta aaaggttgtc ggcgtctttg ccaagcaact gcgcaatatc tgtcattact
     8941 atgctcccgt aaattccgat tggatatcgg ctatggattg tcctggcccg ccttttgcgg
     9001 gcaatcataa tcctggtcgt tacgggcgag tttttccatc ttttggatgt tttttcagcg
     9061 ttttctgttg gctcgattca tcagaaaaaa tgttagcgcg gtcaagtttt caccgcaaag
     9121 gtatttaaaa ggtattatta agtggtattg tcatcgcgta ccttacatta cctgtcatga
     9181 aggaattaaa agatgaaaac aacagcaaag ctgtcgttca tgatgtttgt tgaatggttt
     9241 atctggggcg cgtggtttgt gccattgtgg ttgtggttaa gtaaaagcgg ttttagtgcc
     9301 ggagaaattg gctggtcgta tgcctgcaca gccattgcgg cgatcctgtc gccaattctg
     9361 gttggctcca tcactgaccg ctttttctcg gcgcaaaaag tgctggcggt attgatgttc
     9421 gcaggcgcgc tgctgatgta tttcgctgcg caacagacca cttttgccgg gttcttcccg
     9481 ttactgctgg cctactcgct aacctatatg ccgaccattg cgctgactaa cagtatcgct
     9541 tttgccaacg tgccggatgt ggagcgtgat ttcccgcgca ttcgtgtgat gggcactatc
     9601 ggctggattg cctctggtct ggcatgtggt ttcttgccgc aaatgctggg gtatgccgat
     9661 atctcaccga ctaacatccc gctgctgatt accgccggaa gttctgctct gctcggtgtg
     9721 tttgcgtttt tcctgcccga cacgccgcca aaaagcaccg gcaaaatgga cattaaagtc
     9781 atgctcggcc tggatgcgct gatcctgctg cgcgataaaa acttcctcgt ctttttcttc
     9841 tgttcattcc tgtttgcgat gccactggcg ttctattaca tctttgccaa cggttatctg
     9901 accgaagttg gcatgaaaaa cgccaccggc tggatgacgc tcggccagtt ctctgaaatc
     9961 ttctttatgc tggcattgcc gtttttcact aaacgctttg gtatcaaaaa ggtattattg
    10021 cttggtctgg tcaccgctgc gatccgctat ggcttcttta tttacggtag tgcggatgaa
    10081 tatttcacct acgcgttact gttcctcggt attttgcttc acggcgtaag ttacgatttt
    10141 tactacgtta ccgcttacat ctatgtcgat aaaaaagccc ccgtgcatat gcgtaccgcc
    10201 gcgcaggggc tgatcacgct ctgctgccag ggcttcggca gtttgctcgg ctatcgtctt
    10261 ggcggtgtga tgatggaaaa gatgttcgct tatccggaac cggtaaacgg gctgactttc
    10321 aactggtccg ggatgtggac tttcggcgcg gtgatgattg ccattatcgc cgtgctgttc
    10381 atgatttttt tccgcgaatc cgacaacgaa attacggcta tcaaggtcga tgatcgcgat
    10441 attgcgttga cacaagggga agttaaatga aaacagaacg tattctcggt gctctttatg
    10501 ggcaggcgtt aggggatgcg atggggatgc cctccgagct ttggccacgc agccgcgtta
    10561 aagcacactt tggctggatt gaccgttttc tgcctggacc aaaggagaat aacgcggcct
    10621 gttattttaa ccgcgccgaa ttcaccgacg atacctcgat ggcgctgtgt ctggcggatg
    10681 cgttactgga acgtgaaggc aagatcgatc cggatctgat tgggcgtaat attctcgact
    10741 gggcgctgcg tttcgacgcc tttaacaaaa acgtactagg tccgacctcg aagattgcgc
    10801 ttaacgccat tcgcgacggt aaacccgttg ctgaactgga aaacaacggc gtgaccaacg
    10861 gcgcagcgat gcgcgtctcg ccattaggtt gtttgcttcc ggcgcgtgat gttgattcct
    10921 ttattgatga tgtggcgctg gcgtcgagcc cgacccataa atccgatctg gcagttgcag
    10981 gtgcggtagt catcgcatgg gcgatttctc gtgccattga cggagaaagc tggtcagcga
    11041 ttgttgattc cctgccttca attgcgcgac atgcacagca aaaacgcatc actaccttca
    11101 gcgcctcact ggcagcacgt ctggagattg cgctgaaaat tgtgcgcaat gccgacggca
    11161 ccgaatccgc cagcgaacag ctttaccagg tcgttggcgc aggtaccagc actattgagt
    11221 ccgttccgtg cgccattgcg ctggttgaac tggcacaaac cgacccgaat cgctgcgccg
    11281 tcctgtgcgc taaccttggc ggcgacacag acaccatcgg cgctatggcg acggcgattt
    11341 gcggcgcgtt gcatggcgtt aacgctatcg atcctgcatt aaaggcggaa ctggatgcgg
    11401 taaatcagct tgatttcaac cgctatgcca cagcgctggc gaaatatcgt caacaacggg
    11461 aggcggtatg agcggcgctc gattacacac gctgctgcct gaattaacct cgcgtcagcc
    11521 ggtgatggtc gtcggcgcgg cggtcattga tgtgatcgcc gacgcttatg ccctcccctg
    11581 gcgtgggtgc gatatcgaac tgaagcagca gagcgttaac gttggcggct gcgcactgaa
    11641 tattgccgtg gcgttaaagc gcctcggcat cgaagcgggt aatgccttgc cgctcggtca
    11701 gggcgtgtgg gcggagatta ttcgcaaccg gatggcgaaa gaggggttaa tcagcctgat
    11761 cgataacgcc gaaggcgata acggctggtg tctggcgctg gttgagccgg atggcgaacg
    11821 cacttttatg tcattcagcg gtgtggaaaa tcagtggaat cgccagtggc tggcgcgatt
    11881 aaccgttgcg cctggcagcc tgctctattt ttccggttat caactggcct cgccctgcgg
    11941 cgaattgtta gtggaatggc tggaaaagct gcaagacgtg acgccgttta tcgattttgg
    12001 cccacgtatc ggcgatatcc cggacgcatt actggcgcgg atcatggcct gtcgaccttt
    12061 agtatcactc aatcgtcaag aggctgagat tgccgccgaa cgttttgctt tatccgcaga
    12121 gataacaaca cttggcaagc aatggcagga gaaatttgcc gcgccgttga tcgttcgtct
    12181 cgataaagag ggtgcatggt atttcagcaa cgacgcttct ggctgcattc cggcatttcc
    12241 aacgcaagtt gtagacacca ttggggcggg cgacagtcat gcaggtggcg tacttgccgg
    12301 gctggcctct ggtctgccac tggcggatgc cgtattactg ggcaatgcag tggcgtcgtg
    12361 ggttgtcggg catcggggcg gtgattgtgc gccaacgcgc gaggaactac tcctcgcaca
    12421 caaaaacgta tagatcgctg cgacagtggc taatgctgta ctcaataggc cgttgctgtt
    12481 ggtcaagcgc cacttgcttg atcaccagca ccggtatttt gctatctaac tgaatatgtg
    12541 actgaaactc ggcatccggc atccgggcgc taacgcggga acgcgtacgt tgtgggtaaa
    12601 tgtgctggct gcggaaatag tcataaagtg aaatcccgat ggcatcaaca tcatgaatta
    12661 aatgcgccgg aacccacgat tcctcaatcg ataccgcttc ttcatcgaca tagcgaatac
    12721 gcttaagcaa aaagacatca cttcctgctt cgaccgccag ctgctgcgcg acttcttcgg
    12781 gacatttcac gacacgcttg ttaacccata gcgtatcggg ctttttcccg cgcaatacca
    12841 cctgctgaga aaaacctcgc gcttctttca acgagtattc gaagatgttg ttgatctgcg
    12901 tgccgtaacc gcgcgaacgc gtcaccacac cctcttcttc cagcgcctgc atcgccttgc
    12961 gcaccgtaat gcgcgaaacg ccggttaact gacttaaatc acgctcaccg ggcaaaatat
    13021 tgccatgctc cagcaccccg ctgcgcacgg cattttttac cgtttcggca aacttaatat
    13081 aaagcggcgt gttatccgcc gctaaaatac gctcgttcag ttgggcaatc agctgggtat
    13141 gcgcttgttc catttgtctt tttcctgccg gggagtctgg tgggagtata tcactgcttc
    13201 ttatttaagg cgtttcttta atcccatcaa cgaatgcctg caactcctcc accgtgccat
    13261 gaaatacatt aaaatccacc ggaccattga tgccatctac ctgtccacgg tcggaatgct
    13321 gccagaagcg ccaggccatg ccgtcattgt ccggacgacg ttgataatag tgagccaccc
    13381 accacggata ttcattgaaa tagcccgcca gattggtgtg ataaaaaacg gctcctgagt
    13441 aaataatcgg cttttttccc gtactttttt cgaccatttt tagccactga cttactcgtt
    13501 tgcgtaattc ttttgccgat aattttcccc gttcttctac gtccagtacc gcagggaaat
    13561 cgccttgtga gaaatccacc gtttgcagaa ataatcgtgc ctgaaccggc gctgctaccg
    13621 acggggagaa ataatgatac gccccgcgca gcaggccatt ttcgcggctt agttgccagt
    13681 tacgcgaaaa atacgggtcc accagctttt cgccttccgt cgccttaata aaagcaaact
    13741 gtaagcggat accgttgtcg cgcatttttg ccacccgctg ccagtcgatc cgctcctgcc
    13801 agcgagaaac atctattccg tgaatggtgt aactggcagg aatgcgaata gcgaaagatt
    13861 taaccgggcg ataaccgtag aaatggatgt agtctgtaat ctgaactgct gtcgaataga
    13921 aaaaattcac cgtctgacgc ggataaattg caacgatgga aattagccca agagcgcata
    13981 atagagaggt gagttttttt cgactggtaa ttcttagctg cattggtttc atccctgaat
    14041 gtcagtgcca aaggctgaca ataaccaaag cagctatagt acggtgctca ccaccaggcg
    14101 tggaagtggt gaaccggacc aataccgtga ccaacttcca gcgtgtcggc ctgggctaat
    14161 gctgatgaaa gccagctttt tgcctcctgt acggtgtcag cccagtttgt atggcgcggg
    14221 cgtagtgcag ccaacgccgc agagagcgta caaccagtgc cgtgggtgtt tttggtcata
    14281 atgcgcggtg cggtaaaccg ttgttcaccc tcgcgggtaa acagccagtc cgggctttgc
    14341 tcatcatcca gatgaccacc tttcattagc actgcgccac agcccatcgc caacagcgat
    14401 cgcccttgtt ccagcatttc ctgttcggtg cgcgcgtgtg gcgcgtcgag caaggcggca
    14461 gcttcgggca agtttggcgt tattaatgaa acctgtggca ataatcgact gcgcagcgta
    14521 gcaaccgccg aaggtgaaag cagcgggtcg ccgctttttg ccagcataac ggtgtcgagt
    14581 accacgtttt ggatctgata acgttgcaac cgttctgcca ccgcttcaac aatatcggtt
    14641 tccgccaaca taccgatttt agtggtgtcg attcgcacat cgctgaacac cgaatcgagc
    14701 tgggcggcga caaaatcagg ctcaatgcga tacaccgact gtacgccacg ggtattttgc
    14761 gccaccagtg cagtaataac tgagcaacca taagcgccaa gtgccgagaa ggttttaaga
    14821 tcggcctgaa tccccgcacc accactggga tcagtaccgg caatcgtcag agcgttaatt
    14881 cgtttcatgc ctgcacctcc tgcgtcaatt gccagagcgc atcaaggaaa tgtggaacaa
    14941 aactgcctgg cccctcgctt ctggcgactg cgcgctctcc ggcttgtttc atccagtgac
    15001 aggcagatgc gacattttcc agcgtatcgc ctggtaacgc acagcaggca gcgacaaccg
    15061 ccgataatgc acagccagtt cctaccactt tggtcattaa cggatcgcca ccgtgaatgc
    15121 caacggcgcg atggccatcg gtaacataat ccatctcgcc agtgaccacg acgattgcgc
    15181 cagtttcccg tgccagtgtt tgtgcagcgg gtatcgcgtt agctgcggcg tcagtggtat
    15241 ccactccccg tccaccatta gcaatgccag ctaatgccat gatttccgaa gcattaccac
    15301 gtatggcggt tggcttatga gacagaagtt ccagacaaaa acggcggcga taatcgagtg
    15361 caccaaccgc tactggatca agcgtccagg gtgtttgaga gctttttgct tgctcaacgg
    15421 cggcacacat cgactgtgcg cgtagctgcg tcagcgtgcc aacgttaatc agcaacgcac
    15481 tggcaatagc cgcaaactga ctggcctctt cggtttcgat aaccatcgct ggcgatgcac
    15541 cgagcgccag caaggtattg gcagtaaagg tttgcaccac atcgttggtc atgcagtgca
    15601 caagagggga atgttggtga aaaaggtgta acgcgtgcgc agattgcgct gaactcagca
    15661 ggtcgacttg catagtttgc tcctgccata acgtgaagaa gcaatgacct ggtggtccgt
    15721 gacttcccta cgctggcatt atccagatca ggtgatacgg gtatttctca gccttcacgc
    15781 agaagggcac cccgagtcgt ttggttgcga tgataaatcg cagaggagga tggtaatgtc
    15841 cagcgcacgc gttgtaaacg aggaaaaacg gcagttttac aatcgcgtta ataaattatt
    15901 tgatatatga atccagcacc ttcagaacga catccagatc ttcttcacgt tttagctcat
    15961 ccccctggtg aacgatgtgt tccgtcagat gacctttaat cacttcccgc atcagaccgt
    16021 ttaccgcgcc acggatagca gcaatctgtt gtaaaactgc agcgcattcg tgcggttcgt
    16081 cgagcatttt cttgagcgcc acgacctggc cctgaatctt actggcacgc gctttcagtt
    16141 tctgtttatc acggattgta tgagacatgg caacacctgg ttaacaagaa tatgaaaaat
    16201 catagcacta ttaatctact ggggggtagt atcaggtact gggggggagt agaatcagat
    16261 tgccgaatta atactaagaa ttattatcat gaccgaattt acaactcttc ttcagcaagg
    16321 aaacgcctgg ttcttcatcc ccagcgccat cttacttggt gcgcttcatg gcctggaacc
    16381 agggcactca aaaacgatga tggcggcgtt tatcatcgcc atcaaaggca ccattaaaca
    16441 ggcggtgatg ctcggactgg cagcaactat ttcgcatacc gcagtggtct ggttgattgc
    16501 ctttggcggg atggtggtca gcaagcgctt tactgctcaa tcagcagaac cgtggctcca
    16561 gttgatttcc gcagtgatca ttattagcac cgcgttctgg atgttctggc gtacctggca
    16621 cggcgagcgc aactggctgg agaatatgca cgagcatgat catgagcatc atcatcacga
    16681 tcacgaagat caccacgacc atggacatca tcaccatcac gaacatggcg agtatcagga
    16741 tgctcatgca cgagcccatg ctaatgacat taaacgacgc tttgatggta gagaggtcac
    16801 caactggcaa attttgttat ttggcttaac cggtggcctt atcccctgcc cggcagcaat
    16861 taccgtgctg ttgatatgca ttcagttgaa agccctgaca ctgggcgcaa cactggtcgt
    16921 cagtttcagc attggcctgg cgttaacgct tgtcaccgta ggcgttggcg cagcaatcag
    16981 cgttcagcag gtcgcaaaac gctggagcgg atttaacact ctcgctaaac gcgcccccta
    17041 tttttccagt ctgttgattg gcttagtcgg tgtgtatatg ggcgtacatg gcttcatggg
    17101 cataatgcga taactcaatc ctttaggctt gcatgttatc ttcagcccag gatgattctt
    17161 aaatcagcta tttccgctga ctctctgctc gctaaggatg cttttagggc atcctttcat
    17221 ttacactttt tacgaaatca tgggatcact aacaaaatat cgcttgtcag ttatattgta
    17281 tggcaggaaa gatatgcgac tgatattaca gatccccaaa gtggagagtt tatgaccatt
    17341 aaaaataaga tgttgctggg tgcgcttttg ctggttacca gtgccgcctg ggccgcacca
    17401 gccaccgcgg gttcgaccaa tacctcggga atttctaagt atgagttaag cagtttcatt
    17461 gctgacttta agcatttcaa accaggcgat accgtcccag agatgtaccg caccgatgag
    17521 tacaacatta agcagtggca gttacgtaac ctgcccgcgc ctgatgccgg gacgcactgg
    17581 acctatatgg gtggcgcgta cgtgttgatc agcgacaccg acggtaaaat cattaaagcc
    17641 tacgacggtg agatttttta tcatcgctaa aaaaagcccc ctcatcatga gggggaaatg
    17701 cagacacctt gttatttttt attattagcc acttgctcgt cttgcttgtt attagtcgta
    17761 tttcacgttg attaatgcgg ttgcctccag tgcgccagat ttaactttgt ttgtatcgta
    17821 gacgtagtaa ctggcagtta tcggaattga aaccgttgta tctgtaagtt ttgactttgc
    17881 cgcaatttct tttaaatttg tgtatcgttt actgctgtta gtgatattta aaagtgtgac
    17941 tgacgaacca ttactttcaa gaataaatcc tacgccatta tcttccgcac ctttcagtat
    18001 tgtgttgctg ttggttaaca agtcagaact tgataaatac acctgcattt tccgtgatgt
    18061 ttgattttgt tgacctaata aatttttaca acgcagaata atatcccctt gtttactatt
    18121 tattgttgct ttgttacctg tggctggtaa ttgtgaaacg ggaatactgt ctaaagtaat
    18181 taataaatcg tccggtttgc aggttgtggt tatcgggttg tatgacaatg tcagattaaa
    18241 ggaatagaca cctgcatttt gtgccaatgc atttctgtag ctatttacac atgcatccca
    18301 tgacttgcca cctaataaac acagtagaaa ttgtactgcg gcactcccgc gtgtcgatga
    18361 tgaagcactg gataatgtcg caactccatt aatcatcact gaaccgccgc cccccgcaga
    18421 aacattcact tttgcattac tggccgcagg tgtcaccgtt accgaataac cactggtgaa
    18481 actggtctga ttatttgata acgtgatgtt gtttttattg gtccattcaa aatgtaaatt
    18541 taatttaatg gtatcgttat aaagtccaac aataatatcc gacgggctca taggggtgat
    18601 agtatttgta ctcacgcatg acgaatcatt aaaattatgc tcatatgtcg ccatttgtgt
    18661 ttgagtaagt gcaaaatccc cctgaacaac caaattgtgc tgaacagata actcactata
    18721 acatgccgca tagctcacag gcataac
//
LOCUS       DAZFWL020000089        18519 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFWL020000089 DAZFWL020000000
VERSION     DAZFWL020000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.089"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            147..1367
                     /locus_tag="ACHKNP_RS16365"
                     /old_locus_tag="pgaptmp_003273"
     CDS             147..1367
                     /locus_tag="ACHKNP_RS16365"
                     /old_locus_tag="pgaptmp_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU4705389.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1340..1969
                     /locus_tag="ACHKNP_RS16370"
                     /old_locus_tag="pgaptmp_003274"
     CDS             1340..1969
                     /locus_tag="ACHKNP_RS16370"
                     /old_locus_tag="pgaptmp_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGU4705390.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1970..3130)
                     /gene="ybdL"
                     /locus_tag="ACHKNP_RS16375"
                     /old_locus_tag="pgaptmp_003275"
     CDS             complement(1970..3130)
                     /gene="ybdL"
                     /locus_tag="ACHKNP_RS16375"
                     /old_locus_tag="pgaptmp_003275"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HGU4705391.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3239..4327
                     /gene="hcxA"
                     /locus_tag="ACHKNP_RS16380"
                     /old_locus_tag="pgaptmp_003276"
     CDS             3239..4327
                     /gene="hcxA"
                     /locus_tag="ACHKNP_RS16380"
                     /old_locus_tag="pgaptmp_003276"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HGU4705392.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4337..4534)
                     /locus_tag="ACHKNP_RS16385"
                     /old_locus_tag="pgaptmp_003277"
     CDS             complement(4337..4534)
                     /locus_tag="ACHKNP_RS16385"
                     /old_locus_tag="pgaptmp_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU4705393.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4716..6821)
                     /gene="cstA"
                     /locus_tag="ACHKNP_RS16390"
                     /old_locus_tag="pgaptmp_003278"
     CDS             complement(4716..6821)
                     /gene="cstA"
                     /locus_tag="ACHKNP_RS16390"
                     /old_locus_tag="pgaptmp_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HGU4705394.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7002..7415)
                     /gene="entH"
                     /locus_tag="ACHKNP_RS16395"
                     /old_locus_tag="pgaptmp_003279"
     CDS             complement(7002..7415)
                     /gene="entH"
                     /locus_tag="ACHKNP_RS16395"
                     /old_locus_tag="pgaptmp_003279"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HGU4705395.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7418..8164)
                     /gene="entA"
                     /locus_tag="ACHKNP_RS16400"
                     /old_locus_tag="pgaptmp_003280"
     CDS             complement(7418..8164)
                     /gene="entA"
                     /locus_tag="ACHKNP_RS16400"
                     /old_locus_tag="pgaptmp_003280"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HGU4705396.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8164..9021)
                     /gene="entB"
                     /locus_tag="ACHKNP_RS16405"
                     /old_locus_tag="pgaptmp_003281"
     CDS             complement(8164..9021)
                     /gene="entB"
                     /locus_tag="ACHKNP_RS16405"
                     /old_locus_tag="pgaptmp_003281"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HGU4705397.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9035..10645)
                     /gene="entE"
                     /locus_tag="ACHKNP_RS16410"
                     /old_locus_tag="pgaptmp_003282"
     CDS             complement(9035..10645)
                     /gene="entE"
                     /locus_tag="ACHKNP_RS16410"
                     /old_locus_tag="pgaptmp_003282"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HGU4705398.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10655..11830)
                     /gene="entC"
                     /locus_tag="ACHKNP_RS16415"
                     /old_locus_tag="pgaptmp_003283"
     CDS             complement(10655..11830)
                     /gene="entC"
                     /locus_tag="ACHKNP_RS16415"
                     /old_locus_tag="pgaptmp_003283"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HGU4705399.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12199..13155
                     /gene="fepB"
                     /locus_tag="ACHKNP_RS16420"
                     /old_locus_tag="pgaptmp_003284"
     CDS             12199..13155
                     /gene="fepB"
                     /locus_tag="ACHKNP_RS16420"
                     /old_locus_tag="pgaptmp_003284"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU4705400.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(13159..14409)
                     /gene="entS"
                     /locus_tag="ACHKNP_RS16425"
                     /old_locus_tag="pgaptmp_003285"
     CDS             complement(13159..14409)
                     /gene="entS"
                     /locus_tag="ACHKNP_RS16425"
                     /old_locus_tag="pgaptmp_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HGU4705401.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14520..15524
                     /gene="fepD"
                     /locus_tag="ACHKNP_RS16430"
                     /old_locus_tag="pgaptmp_003286"
     CDS             14520..15524
                     /gene="fepD"
                     /locus_tag="ACHKNP_RS16430"
                     /old_locus_tag="pgaptmp_003286"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HGU4705402.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15521..16513
                     /gene="fepG"
                     /locus_tag="ACHKNP_RS16435"
                     /old_locus_tag="pgaptmp_003287"
     CDS             15521..16513
                     /gene="fepG"
                     /locus_tag="ACHKNP_RS16435"
                     /old_locus_tag="pgaptmp_003287"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HGU4705403.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16510..17325
                     /gene="fepC"
                     /locus_tag="ACHKNP_RS16440"
                     /old_locus_tag="pgaptmp_003288"
     CDS             16510..17325
                     /gene="fepC"
                     /locus_tag="ACHKNP_RS16440"
                     /old_locus_tag="pgaptmp_003288"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU4705404.1"
                     /translation="MNESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17322..18455)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKNP_RS16445"
                     /old_locus_tag="pgaptmp_003289"
     CDS             complement(17322..18455)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKNP_RS16445"
                     /old_locus_tag="pgaptmp_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HGU4705405.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4358 a         5245 c         4782 g         4134 t
ORIGIN      
        1 tatataacta cagacagcta ctataacttt catctattta ttcacagcgc gaatgaaaaa
       61 taaaacactc tgctttgcag tgtttacttt ataattaaca gacttatctc actgatcacc
      121 ctgtaacgtt cagagagcgt cttcagatgt ctatttataa aattcctctt ccgctcaata
      181 ttctggaagc tgcacgagag cgtattacct ggacactcaa caccctaccc cgcgtttgtg
      241 tctcattttc tggcggcaaa gactctggtt taatgctgca tctgacagcc gaacttgccc
      301 gacaaatggg caaaaaaatc tgcgttttgt ttatcgactg ggaggcgcaa ttctcttgca
      361 ccattaacta tgttcagtcc ctgcgcgagt tgtacaccga tgtcatcgaa gagttttact
      421 gggttgcgct cccgcttacg acgcaaaatt ccctttcaca ataccaaccc gaatggcagt
      481 gctgggaacc tgatgtcgaa tgggtgcgtc agcccccgca agatgcgata accgatcctg
      541 actttttctg cttttaccag ccaggcatga ccttcgaaca atttgtacgt gagtttgccg
      601 aatggttttc acaaaaacgt ccggcggcga tgatgatcgg catccgtgcg gatgagtcct
      661 acaaccgttt tgtcgccatc gccagtttaa ataaacaacg ttttgccgac gataaaccat
      721 ggactacggc cgcaccaggc ggtcatagct ggtacattta ccccatttac gactggaaag
      781 tggctgatat ctggacctgg tatgcaaatc atcaaaatct ctgcaaccca ctgtataacc
      841 taatgtatca ggcaggcgtt cctctgcgtc atatgcgaat ttgcgaacct tttggcccgg
      901 agcaacgaca aggattatgg ctctatcacg ttatcgaacc ggatcgctgg gctgctatgt
      961 gcgcacgagt cagcggggta aaaagtggcg gcatttacgc cggacatgat aatcatttct
     1021 atgggcatcg gaaaatcctc aagccagaac atttagactg gcaagaatat gcattattgc
     1081 tgctcaatag catgccggaa aaaacagctg agcattaccg caataaaatc gccatttatt
     1141 tgcactggta tcagaaaaaa ggcatcgagg ttccacaaac ccagcaaggg gacattggcg
     1201 cgaaagatat cccctcctgg cggcggatat gcaaagtttt actcaataac gattactggt
     1261 gtcgggcatt atcatttagt cctacgaaag cgaagaacta tcagcgttat aacgaacgga
     1321 taaaaggaaa acgtcaggaa tgggggatac tatgcaacaa cgattaacgc aggatttaac
     1381 gcaatttctc gccagcttgc cggaagatga tcgcatcaaa gcgattaatg aaatccgcat
     1441 ggcgatccat caggttagcc cctttcgtga agagccagtg gattgcgttc tgtgggttaa
     1501 aaacagccaa cttatgccta atgattacaa cccaaacaac gtggcaccac ccgaaaaaaa
     1561 actgctgaaa aaatccattg aaatagatgg ttttacccaa cccattgtgg tcacgcatac
     1621 ggataaaaat gccctggaaa tcgtggatgg ttttcaccgc catgaaattg gtaaaggctc
     1681 atcatcatta aaattacgct taaaaggtta tttgccggtg acctgtctgg aaggtacgcg
     1741 taatcaacgc atcgccgcga ctattcgtca taatcgcgct cgtggacgcc atcaaatcac
     1801 cgccatgtcg gaaatcgtcc gtgaacttag ccagttggga tgggacgata ataaaattgg
     1861 caaagagcta ggaatggaca gcgacgaagt gctgcgcctg aagcaaatta acggcctgca
     1921 agagctgttt gccgaccgtc aatattcccg cgcctggacg gttaaataac taaagctggc
     1981 gcaggcgttc agctgctgcc agcaacgtcg attccttctt ggcaaaacag agacgaatca
     2041 gtttatgtgg gaagggatcg gcgcaaaaca ccgacagcgg aatcgccgct acgccgtgct
     2101 cctgcgtcag ccactggcaa aactcaacat catccagggt agaaacctcg ctgtaatcca
     2161 ccagcaaaaa gtatgtacct tcacacggta aaatctccag ccggctttca tttaaagcat
     2221 tcaccagaat atcgcgcttc tggcgataaa agtccggtaa cgcaagataa tgctcaggtt
     2281 ctgcacgtag catatcagca agcgccagct gtgccggggt attcaccgaa aaggtcagat
     2341 actgatgtac cttgcgaatt tcggcgctga tgggcgctgg cgcaacacaa taacccactt
     2401 tccagccggt catatgatag gtcttgccaa atgaagaaac cgccactgcc cgctcacgca
     2461 gctgcggatg cgccagcaca ctggcatggc cctgttgtga aaagttgatg tgctcgtaga
     2521 cttcatcgct aatgacaaaa atctcgtgcc cggcgatcgc ctgccacaaa gcggcgaaat
     2581 cagcctgctg ccagacagtt gcactggggt tatgcggagt gttgaggatc accagtctgg
     2641 tgcgctcgct taataatgcg gcaaattcct gccagtcaac gcgaaaatgc ggtggttgca
     2701 gtgccatacg cttcactatt cccccagaaa gcgcgatggc gggggagtaa ctgtcatagc
     2761 tgggatcaaa acaaatcact tcatcgccat tgcgcaccag tgcggtaatc gccgcgtata
     2821 acgcttccgt cgcccctgcc gttacggtga tatcgctatc ggcatctggt tgatagccat
     2881 acaaacgttc cgttttctga gcaatcgcct cgcgcaaggc ctgcacgccg gtcatgggcg
     2941 cgtattggtt tgccccctgg gcaacgtggt acgccagccg ctcctgtaaa tagcgcggac
     3001 catcaaaatc aggaaagcct tgcgacaggt taatcgcctg gtgttgctgc gccagcgcgc
     3061 tcatctgggt gaaaatagtg gtgccaagtt gtggaagttt gctttgtgga atcagagggt
     3121 tatttgtcat ttttattgta cctgacgaag ttgcgtgttg aaggcactat aacacgatgc
     3181 tagtatttgg caatcaagac gtttagatgt ctaaataaaa caataaggac aacacaacat
     3241 gcctcacaat cctatccgcg tggtcgtcgg cccggctaac tacttttcac atccaggaag
     3301 tttcaatcac ctgcacgatt ttttcactga tgaacaactt tctcgcgcgg tgtggatcta
     3361 cggcaaacgc gccattgctg cggcgcaaac caaacttccg ccagcgtttg gacttccagg
     3421 ggcaaagcat attttgtttc gtggtcattg cagcgaaagc gatgtacagc aactggcggc
     3481 tgagtccggt gacgatcgca gcgtggtgat tggcgtcggc ggcggcgcac tgctcgacac
     3541 cgcgaaagcc ctcgcccgcc gtctcggtct gccgtttgtt gccgttccga cgatcgccgc
     3601 cacctgcgcc gcctggacac cgctctccgt ctggtataac gatgccggac aggcgctgca
     3661 ttatgagatt ttcgacgacg ccaattttat ggtgctggtg gaaccggaga ttatcctcaa
     3721 tgcaccgcaa caatatctgc tggcggggat cggtgacacg ctggcgaaat ggtatgaagc
     3781 ggtggtgctg gctccgcaac cagaaacgtt gccgctaacc gtgcgactgg ggatcaataa
     3841 tgcgcaggcc attcgcgacg tattgttaaa cagtagcgaa caggcgctga gcgatcagca
     3901 aaatcaacag ttaacgcaat cattttgcga tgtggtggat gctattattg ctggtggtgg
     3961 gatggtcggt ggtctgggcg atcgttttac gcgtgtggcg gcggctcatg ccgtgcataa
     4021 cggtctgact gtcctgccgc aaaccgagaa atttctccac ggcaccaaag tcgcttatgg
     4081 cattctggta caaagcgcct tgctgggtca ggatgatgtg ctggcgcaat taaccggggc
     4141 gtatcagcgt tttcatctgc cgaccacgct ggcggagctg gaagtggata tcaataatca
     4201 tgcggagatc gacaaagtga ttacccacac cctgcgtccg gtggaatcca ttcattacct
     4261 gccagtcacg ctgacaccag atacgttgcg tgcagcgttc gaaaaagtgg aatcgtttaa
     4321 agcctgacgt gcaaatttag cagcagcgcg cgccgccttt tccaccgtag cgcgcgtcct
     4381 gccgctcacg gaaaaactct tcgtaggtca tcggcgtttg atcgggatgg ttaacccgca
     4441 tatgttcgac atagttgtcg taatcaggca taccaatcat cagcttcgcc gcctgaccta
     4501 aatattttcc ggcttttgcc agtgaatcaa acatcttggc ctccgctaac catgttgctg
     4561 aaagagtgcc ggatgcggcg tgaacgcctt atccggccta caaatgctat caacgcgctc
     4621 ggtcagtccc ctcgccccat cggggagagg gttagggtga ggaggaaccc tcggcactat
     4681 gccaacattc gccaacatcc cccctcactc tgactttagt gtgcgccttt tgcctgtgcc
     4741 acgatctcct cgacattttc cggcattggc tcatacggcg tttctttcgc cgttggcttc
     4801 ggatctttca atgccgccag tgccgtctta atcgagaaca gcgccagaac caccacgacc
     4861 accataaaga agatggttaa cccggcatcc agacggttgt tgaacaccag ttgcgccagc
     4921 tgtgactcag tatactgcga cggaatattg ccgctgtcga tcattgcctg gaacttatta
     4981 gcaatggcca ggaagccgac tttcgcatcc gggctaaacg ctttctgcca gcctgcggtc
     5041 agggtacaaa tcagcagcca ggccgttggt accagcgcca cccaggcgta acgttgacgt
     5101 ttcatcttga acaacaccac ggcacagagc atcagcgcca tccctgccag catctggttg
     5161 gcaataccaa acagcggcca cagagtgtta atgccgccca gcggatcgac cacgccctga
     5221 tggaggaagt agccccacgc cagtacgcac agcgctgttg ccagcaggtt agcaggcagt
     5281 gaatcggtcc gcttcaggcc cggagaaacc acgcccagca gatcctgcaa cataaagcgc
     5341 gcagcacgcg tacccgcatc taccgccgtc agaataaaca gagcttcaaa cagaatggcg
     5401 aagtgatacc agaacgccac atccatcata ccacccagcg caccgtgcag gatgtaagcc
     5461 atccccaccg ccagcgtcgg cgcaccgccc gcacgggaaa tgatcgactg ttcacccact
     5521 tcgctggcaa tctggttgag cgtatctggt gtaatgctaa agccccagct actcaccacc
     5581 tgcgcggcag aagcgactac atccgccgtc cctgccggag ccagcaccgc cattgggctg
     5641 ttcatggcaa aatacacgcc cggatcgatg atacaggcag aaaccagcgc cataatcgcc
     5701 acgaaggatt ccatcaacat cccaccgtag ccgataaagc acgcctgccc ttcgttcgcc
     5761 agcatcttcg gcgtggtgcc ggaagagatc agcgcatgga agccagacac cgcaccacag
     5821 gcgatggtga taaacaggaa cgggaacagg ttaccggtcc ataccgggcc agtgccatcg
     5881 acaaatttgg tcagcgcagg catggtcagc gtcgggcgca taatcaaaat gcctaccgcc
     5941 agaccgacga tagtcccgat tttcaggaag gtcgagagat agtcacgcgg ggccagcagc
     6001 aaccacaccg gcagcaccgc cgccacaaaa ccgtagccca ccagcatcca ggtcagctgc
     6061 acgccggtaa agtcaaagta cggtgcccag gtcggacttt ctgccaccca gccgccagag
     6121 ataatggcga aaatcaggaa caccaggcca atcactgaca cttcgccaat gcggcccgga
     6181 cgcagatagc gcaggtagat tcccataaaa atcgccagcg gaatggtgaa cgcaacggta
     6241 tacgttcccc acgggctatg agtcagggct ttcaccacga tcatcgccag tactgcaagg
     6301 ataatgacca tgatcataaa gcaggctacc agcgcaatca ccccggcagt tggccccatc
     6361 tcttctttga ccagctcacc cagcgatcga ccgtcacggc gcgtagaaac aaacagcacc
     6421 atgaaatcct gcaccgcacc agcgagaacc accccggcga gcagccagat catccctggc
     6481 aggtaaccca tttgcgccgc cagtaccggc ccaaccaacg gacctgctcc ggcaatggcc
     6541 gcaaaatggt gaccgaacag cactttcttg tccgtcggca catagtccag cccgtcgtta
     6601 tggcgcaccg ctggcgtcat acgcgtcggg tcaaccgcca gcacattttt ggcgatatac
     6661 agcccataaa aacggtaagc gatcagatag atacagaccg acgccaccac aatccacagc
     6721 gcgttgatct gttccccacg atttaaagca atgtatccca gagcaaatgc tcccattaca
     6781 gagagcactg tccagacgag gtatttccct gatttgttca tagttgttat ccgtgtgcgt
     6841 gtccatagag atgttacatt ttgtttctat aacatctttg taagatttaa caacacggta
     6901 accatgtaaa tcggagttga aaccaatatt taacttaaca atgttaactc gatcactccg
     6961 ttaaccgagt tttcctgcaa tctcatttaa ttctgtcggg ctcatcccaa aactgccgta
     7021 cccagccgac aagtgcagca acgccgcccc tgttcatcga aaacgacgat ttcccagctc
     7081 tgattttgcc gcccaagatg cagcggctgg cagacgccgc gtaccttgcc ttcagacacc
     7141 gggcgatgat gggtggcgtt aagttccgta cccaccacac actgcccgtc acgggtcatc
     7201 ataaatccgg ccatcgatcc cagcgtttca gccagcgccg ccgacgcgcc gccatgcagc
     7261 aggccaaacg gctgatgagt acgggtatca accggcattt cggcttccag cacatcatct
     7321 cccagacggg tatacacaat tcccagatgc gccaccattg tgttatcgct ggtggcgttc
     7381 agttcgtcga gcgttaaatg gcgtttccag atcatgctta tgcccccagc gttgagccgc
     7441 catcgaccac aatatcctgt agggtgatat ggctggcgag gtcagaggcg aggaacaaaa
     7501 tcgtgttggc gatctcttgt ggacgggcga ttttccccag cggaatgccg agtttaaact
     7561 gctcgccaaa gccgcgaata cgctgttctt cggcgtcatc gctcacccac agcgtgcgtt
     7621 gcatatcggt gtcggtggag ccaggcgaaa ccacattaca gcgcacgccg ctacccgcca
     7681 gttccagccc gacgctcaac gccaggcttt tcagcgccgc tttcgatgcg ccataagcac
     7741 tcataccaat acgcggcgtg tgtgcggcgt cggacgccac agtgacaatc gccccgcccc
     7801 gctgacggcg aaactggttc atggtttgct ggaacaggtt aaacgcaccg ccgacgttaa
     7861 ccgcaaaagt ctgctgccag tcctctttac tgagctgatc ggtcgcgccc atgcgcaaaa
     7921 ttcccgccgc attgatcagc acgtccagtc gctccgtttc agctaacagt cgctgacata
     7981 cctgcgcgac ctgcgcagca tcggcaacat ccatcacttc ggtcgcaaag ggatattgct
     8041 cctgagcgaa cgcttgatca aaaccggtaa ctttcgctcc cgcctcaaca aacgccagcg
     8101 ccgtggcgta gccgatacct ttacctgcgc cggttaccca gacattttta ccgctgaaat
     8161 ccattatttc acctcgcggg agagtagctt ccaccaggcg tcgatggtcg ggtttttcgc
     8221 cagcatgaca aagtcgatat caccatgcac tttgcgccag cgcgccgcca gcgccatcat
     8281 gcgcaccgaa tccagaccgt agtcgatcag gttgtcgtca tcgaacggtt catcggactc
     8341 gtccagcaac ggcaggataa cctcacgcag cgccgctttg ctggcgggga caggtgctgg
     8401 cagtaattct tcagtcatca ccacccggcc agaccgcccg gccacatatt tcagcgacat
     8461 caaatgctcg tcacggctga aatcggccag cgcatccgcc accataaacg gtttaatatc
     8521 gcgcataaat gcgtcggttg cggtggtcat acagccaatg tgggcatata ccccggtaat
     8581 aatcagctgg ttacgtccac tctctttgag catttgctcc agcggagaac gatgaaacgc
     8641 gctgtagcgc cacttcacca gcaccgtgtc gtcggcatct ggcgtcaggc gatccaccac
     8701 cttttgctgt tccggcgaac gggtcaggcc cggcccccac atatcattca acagcgcccg
     8761 atcttcatcg ctctgctctt tcggctgggc ggtgtaataa accggaatat tgtgctgttt
     8821 gcagtagtcg cgcagcgcag caatattcgc gatcacctgc tccatcatcg ggcagttctc
     8881 gccccagaag ctgacaaaat agtcctgcat atcatggatt aacaacgcgg cacgttgcgg
     8941 ttcaaaggcc cagtcaactt tattcttagg gatatcgtga gactccggca gtgcgtaagc
     9001 ctgtaatttt ggaatagcca tcgtgttctc tccttcaggc tgatgcgcgt gacgccagcc
     9061 actgacgtaa ttgtttttta tcgactttcc cgaccgccgt cagcggaagt gaatctacac
     9121 actccacgcg atccggtaat ttaaattcgg caataccctg ttcacgcagg aaacgacgca
     9181 cctgcaccgc gcgcagcggc tcttttacca ccagataagc gcagcttttt tcgcccatca
     9241 gctcatcttc catgctcacc agtgcggcgt agatcaccgc cgggtggcgc agcagcaggt
     9301 tttcgatctc ttcggcagcg atcttctcac cgccacggtt gatctgatct ttctcgcgcc
     9361 cctgcacggt gatgtacccc tctggatcaa tagagatcag atcgccggaa cagtaaaaac
     9421 cgttggcatc aaaggcgctg gcattgtgct gcgggctttt gtaatagcca cggaaggtgt
     9481 acggaccgcg ggtcatcagg cgtccaactt ccccttgcgg cagtggattt ccttcggcat
     9541 cggcaaccca cacttcatcg tccggacaca ttgggtaacc ctgggtatgg ataattttct
     9601 ccgcgctatc atcaagacgg gtgtagttca ccagcccttc cgccatgcca aacacctgct
     9661 gcaactggca gccaatctca gcgggaatac gcgccgcaag ggtggcagaa agacgtgcgc
     9721 cgccgacctg taacagtttc agcgaggcaa gctgcgcccg gctttcacct tcggtcagcg
     9781 cctgcaacca caggctgact gccggtggca ccagcgcggt gacgttcacc tgatgttttt
     9841 caatcaatgg gaagcaaagc gtggcgctgg gatcggccgc cagaacaacc gttcctccgg
     9901 caagaaagac gcccagcgat cccggcgaac tcatggcgta gttatgagcc gccgggatcg
     9961 cgcacaggta gcgtgtctgt tgtgtgaact gacaaatctc gacgctacga cgcacgctgt
    10021 agtagtagtc gttatgagtg cgcgggatca gtttcggtgt gccggtggtg ccgccggaaa
    10081 gctggaaata ggccacttca tcagcaggtg atggcgtggc agtaaaatcc tcagccggat
    10141 ggttaatcgc atcctgcaag ttatgctcac cgctgtcgtt gtgcagttgc accacgcgaa
    10201 tggaggaatg ttctgtgacg aaagtattga ggaaatcatc cccgctaaac agcgcatgtt
    10261 ggcgatcggc aatcagcaat gcgggttcaa tctggctggc ataggcgttc agttcactac
    10321 gctgatggct gaacagcgcc agcaccggcg caacgcccag tttcagcagc gcgaaaaagg
    10381 taatatacaa ttcagcgacg ttacccagtt gtaccagcgc ggtttcacca ggtttaatgc
    10441 cctgacggcg taaactacac gcgaggttat ccgccgcctg attcagctcc cggtaactca
    10501 actgtcgctc gccgtcgata accgcgatgc tgtcactcgc agcgtggcga gtcagaatgt
    10561 cggtcagcgg caaatcctgc cagtagcctt tttcccgata gcgacgggca aactcttccg
    10621 gccagcgggt gaatggaatg ctcatcctcg ctccttaatg caatccaaaa acgttcaaca
    10681 tggtagaaag tttgacgcct gtttcgcgcc actcacccaa cggtgacgac gcaggcacaa
    10741 tccccgctcc ggcaaacaga cgcacctgat tttcccgcag cttcgcgcag cggatggtca
    10801 ccacccattc gccgttacct tcgctgtcac accaacccac aatgccgcca aacagttcgc
    10861 ggtcgaatgg ctccagttca gcaataacct gggtcgcggc ctgatgcggg aaaccgctca
    10921 gcgcgggggt tggatgcagc agacaggcca gagtcagtgc gttttcttgc gaattcgctt
    10981 taccttcaaa gggagttgcg agatgccaca gcgtcggcgt ggtaatcaat tgtggagagg
    11041 agggaacgtg taactcacta ctgcgttcgc gcagtacctc tttcatcgcc tgagtcacca
    11101 gttcatgttc atggcgatct ttttctgacg ccagcagacg attacccgct tcgcgatcga
    11161 gcacttcatc cggctgacga cgcgcggaac cggctaacgg aatggagcta aaacgctcgc
    11221 cgtctttacg tagcagcagt tccgggctgg cccccagcag gacgccacca tcagccagcg
    11281 ggacatggaa gttgtaacta accgggtttt gcgcaatcaa ccgttccagc aatacgccac
    11341 tatcaatggc ggcgtcagtg gtgatatcaa tcaatcgtga caacaccact ttgtcgacct
    11401 gcggcgtggc ggtaagtgcg gcggcgcggg caaccatctg ttcaaacgtg gtttgttcag
    11461 gaattgcctg gcgttccacc acgttcagcg actggctgcg ggtaaaacgg cgggctgagg
    11521 tctgtttttc ctggcgggag aacgactgcc aggattcggg aatatacagc gacgaaggct
    11581 gacgtggatc gaagggaatc gccccgacca tcaccggatt tttgatgccc tgcgctttgg
    11641 catcggcaaa cagcgcggcg agtttttgct ggaagggact gtcgggcgaa tccccgttca
    11701 cagccggttc atcgaagcgg gcgaaacatc ctgacgtcgt aaaactgcgg tacggcgaca
    11761 taaagaaaaa gcgattgggc gcaagtgttg ccatggtctg ctgtacttcc tcagccagtg
    11821 acgtatccat atcatcctcc acaaaatgat aaaggcttta ataatgatta tcatttatat
    11881 tttcggtcgc taacctaaaa gcaaacgcgc actatgtcaa ctcttgaggt aacgcaatct
    11941 gcaactcggg gttgcatctg gttgtggtta caatgaaaat gagaagcatt attgatggat
    12001 tcgcataagc gcaatgtgat gtcctgcgcc gttctgcccc ctctcccttc cagggtgagg
    12061 gctggggtga gggttaatgt tcgcaccagt gctggctgtt cccctcaccc tctccccaaa
    12121 ggggcgaggg gacggattgt gcgctttgtc gaatttgtca ttacgcccat aaccttatta
    12181 ataacaggaa gctgatttgt gagactcgcc ccgctctacc gcaacgccct tctattaaca
    12241 ggacttttgc tttcaggaat agccgcagtt caggccgctg actggccgcg tcagattact
    12301 gacagccgtg gcacacatac actggaaagc cagccgcagc gtattgtttc caccagcgtc
    12361 accctgaccg gctcactgct ggcgattgat gctccggtga tcgccagcgg cgcgaccacg
    12421 ccgaataacc gcgtcgcgga tgaccagggc tttttacgcc agtggagcaa ggtggcgaaa
    12481 gaacgcaaac tgcaacggct ctatatcggc gaaccgagca ccgaagccgt tgccgcgcaa
    12541 atgccggatc tgattttaat tagcgcaacc ggcggggatt cggcgctggc actgtatgat
    12601 cagctttcca ccatcgcccc gacattaatc atcaattacg acgacaaaag ctggcagtcg
    12661 ctgttaacgc agcttggcga aattaccggg catgagaaac aagcggcaga gcggattgca
    12721 cagtttgata agcaactggc agcggcgaaa gagcaaatca aattaccgcc gcagccggtc
    12781 actgccattg tctataccgc cgctgcacac agtgccaatc tctggacgcc ggaatcagca
    12841 caagggcaga tgctggaaca actcggcttt acgctggcga agttgcccgc agggttaaac
    12901 gccagccaaa gccagggcaa acgccatgac atcattcagc ttggtgggga aaatctggct
    12961 gctgggttaa atggcgagtc actattcctg ttcgccggtg atcagaaaga tgccgatgcg
    13021 atttatgcca atccactgct cgcgcacctt cccgcagtac aaaacaagca ggtttatgcg
    13081 ctgggaaccg agacgttccg tctggattac tacagcgcca cgcaagtgct ggaacgactt
    13141 aaggcgctgt tttaagcatt aactgtcaga cgctgtcacc tgcggcggcg tctggcgaaa
    13201 acgtcgcaac tccaccagca ccagcaataa caacacgccg ataatcaaca aaccaaaacc
    13261 gcttgcgctt gcggaagcaa ccggtgtcat catcgcgccc aaaccaccca gcagcgccgc
    13321 gcctatggca tcgcccgtca cgttctgcgc cgtccacaaa ccgttaatcc gccctaacat
    13381 cgcttccggg gtttgcgttt gcagcattgt atattgcagc aacgaactaa ccgcgctcag
    13441 ccagccgaac agcgccagac aaaccacgcc taaaatccac atcggcatca ggccaaacag
    13501 accaatggcg aggaacgatc ccagcgtgga gagcagcatc aataaccctg gtcgcgcact
    13561 atgtgccagc ttcccgctgg ttaacgcacc aatagccgcg ccgagcggga tcgccgcgta
    13621 gagaaaacca atctgtgccg ctgacatctg ccagttgtca gccagcgccg gatacagtac
    13681 ccgcaccgcg ctcgccatcg tcaataaacc acccagcagc gcaatcccgc ccaccagcgg
    13741 gctggcaagc agaaaacgaa atcctgccag taatgatttc aacggatgct cacgcggctg
    13801 cggtggcggt ggcaacgccg gaaggcttaa caacggtagc aaggtaataa acgtgcccgc
    13861 cgccgccagc ccgtagttcc aggctacgcc accggtcgcc agcaataaac cgccaatcat
    13921 gggcgaaatc accgacccca gacgcacggt caacatggtg atcgccccgg cctgcattaa
    13981 gttttcacgc cctaccagtg ctggtgtcgc cgccaatagc gccgtaacgc caagcgatgc
    14041 gaaaaaacca tcccataaac caagtaaata gattgccagc aatgacggct ccggcagcag
    14101 tgcattaagg cacagtccaa tgaagccaat gccacaggtg ccgcgcgcca gcaaaatcac
    14161 ttttttgcgc tcatagcgat ccgccagcac accgccaacc atcaggccaa caaacatcgc
    14221 gccgccggtc agcgtcaccg aaagccccac ctgccaggta gaatgcgtca tcatctggat
    14281 ctgcaccggc accgcgacgc cgagcaaacc cagagacaca attgagataa aacgagcgag
    14341 gaatactgcg cgaaacgccg ggtgcgtttt caacaggctg aggttaagca gccaggattg
    14401 tttattcatt acaatgcctt gccatcaaat attttaaata cctattctta ggctgcacat
    14461 gctaacatat ccaaataaga tagataacga taattaattt cattatcatg gaagttcgta
    14521 tgtctggttc tgttgccgtg acacgcgcca ttgccgtgcc cggattgctg ttattactga
    14581 ttatcgcgac ggcattaagc ctgctcattg gggcaaaatc actccccgct tccgtagtgc
    14641 tggaggccct ctccggcacc tgccagagcg ccgactgcac catcgtgctc gacgcgcgac
    14701 tgccgcgcac ccttgccggt ttacttgcag gcggcgcgct tggccttgcc ggggcgttga
    14761 tgcaaaccct cacccgaaat ccacttgccg accccggctt gcttggcgtg aatgccggag
    14821 ccagctttgc cattgtgctg ggtgcggcgc tgtttggtta ctcatccgcg caggaacaac
    14881 tggcgatggc cttcgccggg gcgctggtgg cctcattgat tgttgccttt accggcagtc
    14941 agggcggcgg gcagttaagt ccggtgcgtt taaccctggc gggcgtggcg ctggcggcgg
    15001 tgctggaagg gctgaccagc ggcatcgccc tgcttaatcc tgatgtctac gatcaactgc
    15061 gtttctggca agccggttcg ctggatattc gcaatctaca taccttaaaa gtggtgctga
    15121 tcccggtgct gatcgccgga gcaaccgcgc tattactgag ccgcgcgctg aacagtttaa
    15181 gcctcggcag cgacaccgcg acggcgctgg gcagtcgcgt ggcgcgcaca cagttaattg
    15241 gtctgctggc gattaccgtg ctttgtggta gtgcgacggc ggtagttggc ccgattgcct
    15301 ttatcggcct gatgatgccg cacatggcgc gctggctggt aggtgccgat catcgctggt
    15361 cgctgcccgt cacgctactt gctacccctg ccctgctgct gtttgccgat atcatcgggc
    15421 gggtgattgt gcccggcgaa ctgcgcgttt ctgtggtcag tgcgtttatt ggtgcaccgg
    15481 tgctgatctt cctcgtgcga cgtaaaacgc gaggtggcgc atgatttacg tctctcgccg
    15541 attaatcatc acctgtttgc tgttggtttc cgcctgtgtg gttgcaggta tctggggatt
    15601 acgcagcggt gccgtcacgc tggaaacctc gcaggtattc gccgcgctga tgggcgatgc
    15661 gccgcgcagt atgacgatgg tggtcaccga atggcgttta ccacgcgtgc tgatggcgct
    15721 gttgattggc gcggcactgg gcgtcagcgg cgcgattttt cagtcgctga tgcgtaaccc
    15781 gctcggcagc cctgacgtaa tgggctttaa caccggggcg tggagcggcg tgctggtagc
    15841 gatggtgctg tttggtcagg acctgacggc tatcgcgctg gcagcaatgg tgggcggcat
    15901 tatcacttcg ctgttggtct ggctgctcgc ctggcgtaac ggcatcgaca cctttcggtt
    15961 gattattatc ggtatcggcg ttcgtgccat gctggtggcc tttaatacct ggctgttgct
    16021 gaaagcgtct ttagaaacgg cgctaacagc gggtttgtgg aatgcaggat cgctcaacgg
    16081 cctgacgtgg gcaaaaacct cgccttccgc acccatcatt atattgatgc tcattgccgc
    16141 cgccttactg gtacgccgga tgcgcttgct ggaaatgggc gatgataccg cgtgtgcgct
    16201 gggcgtcagc gtcgaacgtt cgcgtctgtt aatgatgctg gttgcagtgg tgcttaccgc
    16261 tgcggcaacc gcgcttgccg ggccgatttc ctttattgct ttagtcgcgc cgcacattgc
    16321 ccgacgcatt agcggcaccg ctcgctgggg gctgacccag gcggcgctgt gcggggcgct
    16381 gttactgctg gcggccgatc tctgcgccca acaactgttt atgccgtatc aacttccggt
    16441 tggcgtcgtt accgtcagcc tcggcggtat ttaccttatc gtcttgttaa ttcaggagtc
    16501 tcgcaaaaaa tgaacgaatc tgtagcccgt ttgcgcggcg aacagttaac cctcggatat
    16561 ggcaaatata ccgttgcgga aaatctgact gtagaaatac ctgatggtca cttcacggca
    16621 attatcgggc caaatggctg cggtaaatcc actttactgc gtactttaag ccgcctgatg
    16681 acgcctgctc acgggcatgt ctggctggat ggcgagcaca ttcaacatta cgccagtaaa
    16741 gaggttgcac gccggattgg tctgttggcg caaaatgcta ccacgccagg cgatatcacc
    16801 gtgcaggagc tggtggcgcg tgggcgttac ccgcatcaac cgctatttac ccgctggcgc
    16861 aaagaagatg aagaagcggt aacgaaagcg atgcaggcca cgggaataac tcatctggca
    16921 gatcaaagcg tggataccct ttctggcgga caacgccagc gcgcgtggat cgcgatggtg
    16981 ctggcccagg aaacggcaat tatgctgctc gacgaaccca cgacctggct ggatatcagt
    17041 catcagattg atctactgga actgttaagc gaactgaacc gcgagaaagg ctataccctg
    17101 gcggcggtgc tgcacgatct taatcaggcc tgtcgttacg ccagccattt gattgcattg
    17161 cgggaaggga aaattgttgc tcagggagcg ccgaaggaga ttgtcactgc tgaactgatt
    17221 gagcgcattt atggtctgcg atgcatgatc attgacgatc cggtagccgg aacgccgctt
    17281 gtggtgccgc tcggacgaac ggcaccttca accgcaaata gttaaactaa gtggtctgcc
    17341 atcatggcat cctgttttct ggatgccatc gcatagcgca ataacacgct accacaagcc
    17401 accatcccgc cgatcagcgc ggaaaggatc acaataatcg ccttacccgg accgtctttt
    17461 ttcactggca atgacgggct taactgatat ttaaacggcg taaaattcac atcgttgata
    17521 tttgcttttg ttaactgctc aacaagatac tgacgattac gtaattcacc gttcagttcc
    17581 gcaacgtcag tgaccgcttt ttcaatttcc agtttgcgtt caataccgtc tgcgccgaga
    17641 gaaatagaga aatcggggtc atctttaacg gcctgaccat tactgtatac gggcttttta
    17701 attcccgccg cgttggcaat gtcgagtgaa taattgaggc gctgaatgtt tgcatcaagt
    17761 tgatttttcg ttttaatgcg atcctgagcc agtttttctt tttcaaactg ggttttgatt
    17821 tccagtttat tacggacgtt ttctagtgac tctttcacca ccaacgcaga gatataatcg
    17881 atatacccgc tcaaaacggt ctgcgcctct tcactggttg gcgcggtaaa acttagcgtc
    17941 caggaggtat acagtgacgg ttcatctttt ttcttactgg cattgtcatc aaccgctttc
    18001 attttttcgc tcaatgcaac aatagcgcga tgcaaatcca gttcatcgat tttcgcctct
    18061 tttaattggt ccatcacata aggtgatgaa cgcaggtact cttccagcaa gctaaccgac
    18121 tgaaacttct tgataaacag gttaaatgct tctgtacgat caattttgat atccagatcc
    18181 agcactcgaa gcttagtgaa tgttttctcc agctcttgcc actgaacagg ttctggaggc
    18241 gtgacaaccg ccgcgctggt ccatttttgc ggcaggatga aagagatcag caagcctgcg
    18301 caggcaaacg caaaaatgac cgccatgacc gtttttttgg cccgccataa aactgcgatt
    18361 agattaagta aatcaatttc attattactg ggcgacgcca gaggatactc aggaaaatga
    18421 gcgtcacttc cctgtttaat attcagtgat gacatggggt ataacctgaa aatgagtcca
    18481 ataggggaaa tctccacggc gctcacttta ccagttggt
//
LOCUS       DAZFWL020000090        18046 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFWL020000090 DAZFWL020000000
VERSION     DAZFWL020000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.090"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(308..1309)
                     /locus_tag="ACHKNP_RS16450"
                     /old_locus_tag="pgaptmp_003290"
     CDS             complement(308..1309)
                     /locus_tag="ACHKNP_RS16450"
                     /old_locus_tag="pgaptmp_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGU4705406.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1416..1712
                     /locus_tag="ACHKNP_RS16455"
                     /old_locus_tag="pgaptmp_003291"
     CDS             1416..1712
                     /locus_tag="ACHKNP_RS16455"
                     /old_locus_tag="pgaptmp_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGU4705407.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1846..2271)
                     /gene="arsC"
                     /locus_tag="ACHKNP_RS16460"
                     /old_locus_tag="pgaptmp_003292"
     CDS             complement(1846..2271)
                     /gene="arsC"
                     /locus_tag="ACHKNP_RS16460"
                     /old_locus_tag="pgaptmp_003292"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HGU4705408.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2284..3573)
                     /gene="arsB"
                     /locus_tag="ACHKNP_RS16465"
                     /old_locus_tag="pgaptmp_003293"
     CDS             complement(2284..3573)
                     /gene="arsB"
                     /locus_tag="ACHKNP_RS16465"
                     /old_locus_tag="pgaptmp_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HGU4705409.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3627..3980)
                     /gene="arsR"
                     /locus_tag="ACHKNP_RS16470"
                     /old_locus_tag="pgaptmp_003294"
     CDS             complement(3627..3980)
                     /gene="arsR"
                     /locus_tag="ACHKNP_RS16470"
                     /old_locus_tag="pgaptmp_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HGU4705410.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4720..4803
                     /gene="dinQ"
                     /locus_tag="ACHKNP_RS16475"
                     /old_locus_tag="pgaptmp_003295"
     CDS             4720..4803
                     /gene="dinQ"
                     /locus_tag="ACHKNP_RS16475"
                     /old_locus_tag="pgaptmp_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HGU4705411.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4857..6209)
                     /gene="gorA"
                     /locus_tag="ACHKNP_RS16480"
                     /old_locus_tag="pgaptmp_003296"
     CDS             complement(4857..6209)
                     /gene="gorA"
                     /locus_tag="ACHKNP_RS16480"
                     /old_locus_tag="pgaptmp_003296"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGU4705412.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6281..7123)
                     /gene="rlmJ"
                     /locus_tag="ACHKNP_RS16485"
                     /old_locus_tag="pgaptmp_003297"
     CDS             complement(6281..7123)
                     /gene="rlmJ"
                     /locus_tag="ACHKNP_RS16485"
                     /old_locus_tag="pgaptmp_003297"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HGU4705413.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7326..9368
                     /gene="prlC"
                     /locus_tag="ACHKNP_RS16490"
                     /old_locus_tag="pgaptmp_003298"
     CDS             7326..9368
                     /gene="prlC"
                     /locus_tag="ACHKNP_RS16490"
                     /old_locus_tag="pgaptmp_003298"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HGU4705414.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHEEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9376..10128
                     /gene="rsmJ"
                     /locus_tag="ACHKNP_RS16495"
                     /old_locus_tag="pgaptmp_003299"
     CDS             9376..10128
                     /gene="rsmJ"
                     /locus_tag="ACHKNP_RS16495"
                     /old_locus_tag="pgaptmp_003299"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HGU4705415.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10177..11646)
                     /gene="dtpB"
                     /locus_tag="ACHKNP_RS16500"
                     /old_locus_tag="pgaptmp_003300"
     CDS             complement(10177..11646)
                     /gene="dtpB"
                     /locus_tag="ACHKNP_RS16500"
                     /old_locus_tag="pgaptmp_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HGU4705416.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            11812..11865
                     /locus_tag="ACHKNP_RS16505"
                     /old_locus_tag="pgaptmp_003301"
     CDS             11812..11865
                     /locus_tag="ACHKNP_RS16505"
                     /old_locus_tag="pgaptmp_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705417.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(11964..12398)
                     /gene="uspA"
                     /locus_tag="ACHKNP_RS16510"
                     /old_locus_tag="pgaptmp_003302"
     CDS             complement(11964..12398)
                     /gene="uspA"
                     /locus_tag="ACHKNP_RS16510"
                     /old_locus_tag="pgaptmp_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HGU4705418.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12789..13124
                     /gene="uspB"
                     /locus_tag="ACHKNP_RS16515"
                     /old_locus_tag="pgaptmp_003303"
     CDS             12789..13124
                     /gene="uspB"
                     /locus_tag="ACHKNP_RS16515"
                     /old_locus_tag="pgaptmp_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HGU4705419.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13369..14868)
                     /gene="pitA"
                     /locus_tag="ACHKNP_RS16520"
                     /old_locus_tag="pgaptmp_003304"
     CDS             complement(13369..14868)
                     /gene="pitA"
                     /locus_tag="ACHKNP_RS16520"
                     /old_locus_tag="pgaptmp_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HGU4705420.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            15100..16302
                     /gene="yhiN"
                     /locus_tag="ACHKNP_RS16525"
                     /old_locus_tag="pgaptmp_003305"
     CDS             15100..16302
                     /gene="yhiN"
                     /locus_tag="ACHKNP_RS16525"
                     /old_locus_tag="pgaptmp_003305"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGU4705421.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(16618..17670)
                     /gene="yhiM"
                     /locus_tag="ACHKNP_RS16530"
                     /old_locus_tag="pgaptmp_003306"
     CDS             complement(16618..17670)
                     /gene="yhiM"
                     /locus_tag="ACHKNP_RS16530"
                     /old_locus_tag="pgaptmp_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HGU4705422.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     TVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4715 a         4506 c         4603 g         4222 t
ORIGIN      
        1 atgttttaga tgtgtaataa tgaaccaggt taaagtgtat ttaaaaatgt gttgtattag
       61 cgactgaaat ctgcctttta aaaaggagga gggtactgag acaaagagtc attgcttctc
      121 cgataaaatg tcagagaagc atgattacgt ggttattgtc tgttgatgac ttgttgaccg
      181 agccgtcatc attctcaatg cattagttaa atcatttgtg atttttaagg attatcctgt
      241 ctatgatttt ctacttagaa ggatatggca ctaactatgc atcttatagc ttactgctgg
      301 catctattta aaatagcaat tgagtcaaat agcccatcaa gatagccata cctaatataa
      361 ccgtcaggaa tgcagctatc atcggcattc tgaacattga tttaagtaat atcacttccg
      421 tcaggcttgc gccggcactg ccgatgatta atgccattaa tgctcccaga cccattcctt
      481 ttgtcatcag aacagatgcc agagggataa ctgcctcagc ccggatatac agcggaatac
      541 cgacaacggc gcttaatggg atggcgaagg gattatctgc tcctgcatga gcggcaatcc
      601 acgctgaggg aataaagcca taaataaaag atcctattaa tactcctaac aaaagatatg
      661 gcagtacatc tttaaactgt tgcaacgcat ctttggtggc tagttttatg ggattaatac
      721 ttactacagc tttggtactg caacaggtaa ctacggggtt ctcaatggct ttagccgctg
      781 gactacaaca actcactttt atgggcgtat atgtcgttcc cggcgaagtt tttgctggag
      841 cacaacaatt tgctgatgag cttttactgg caatgatatg acgttcaaac cccagagaat
      901 ccaggataat actggcaaga acggagacgc cggctgcgat aatcgcgtac aacaaggtga
      961 ctttccaacc aaaggtcatc cacattagtc cgacgataat gggattaagt aatggtgaaa
     1021 caaataaaaa agtgagagtc ggaccaaacc cggctttcgc tgacaacaat ccacgtaaca
     1081 tgggtattgt cgagcaacta cagaacgggg taacggctcc caacagagca gccaggagat
     1141 agcctctccc ttttctggcc cccatcatct gctggatttt atggtctggc accttttgtc
     1201 ttatcaggct gacaccggca ctaatcacta taaacagcaa cgaaagctcg acggcgagaa
     1261 acacaaacat ctctgcggca tcttgcagca tcgcaagcca actactcata cgtaacctca
     1321 ttggataaat ctatatttcc agaataatcg aaatgaaatt cagatcaaga tatttctggt
     1381 attatcgaaa tatcaaccag acagaggggt gtcatatgca gttggaagaa gtcgctaaag
     1441 cgctgaaaga gctaggtcac ccgacccgtt tgttcatatt taaacatctt gtcaaagccg
     1501 gggaacaagg gttgccggtc ggtgagttgc aaaagcagct tgggatacct ggctccacac
     1561 tcagccacca tatatcagca ctggtatcgg taggactggt caaacaaaac cgtgagagtc
     1621 ggacgctgat gtgtgtttcg caatacgcaa tgctggaagc gataattgaa ttcttacgcg
     1681 aggagtgctg cgtaaatagc aaaattgcct gatgcgctac gctctacata tttcatgcca
     1741 tatattgaat ttgcacggtt ttgtaggtcg gataagacgc gttagcgtcg catccgacat
     1801 aaacaaagcg cactttacca actatcggca gtggatatcg ccgctttatt tcaggcgctt
     1861 acccgcttca tcaaccactt tctcgccatc ttccttggag aatgcgcctt tttgcgcatc
     1921 tggcagaatt tccagcacca cttctgaagg gcggcacagg cgagttccca gcggcgtcac
     1981 cacaatcggg cgattaatca gaatcgggtg ctgaagcata aagtcgatta accgatcgtc
     2041 agtaaattta tcttccgcaa ggcccagctc ctcatacggt tcgacgtttt tacgcagcag
     2101 cgcgcgtacg gaaatcccca tatcggcaat gagtttgacc agttcatcgc gcgttggcgg
     2161 agtttccaga taatggataa tggtcggttc tgtgccgctg ttgcggatca tctccagcgt
     2221 attacgcgac gtgccgcagg ccgggttgtg ataaatggta atgttgctca tatcagtatc
     2281 tcattacaaa gtgaaagaga gacgtagcgc cagcgcagcc agcgtcacaa acagcacagg
     2341 cagggtcatg ataatccctg tacggaagta atatccccag ctgatagtca tattcttctg
     2401 cgaaagtacg tgcagccaga gtagcgtagc caggctacca attggggtaa ttttcggtcc
     2461 caaatcgcag ccaatcacat tggcataaac catcgcttct ttgataacgc cagatgccgt
     2521 gctgccatca atggacaacg cgccaaccag taccgtcggc atattgttca taatagaaga
     2581 gaggaaggcg gtgaggaatc cggtgccgag cgtcgcggcc cacaggccgt tatccgccag
     2641 cacgttgagt acgccagaaa gatattccgt taatccggca ttgcgcaggc cataaaccac
     2701 cagatacatg ccgagcgaga agatgacaat ctgccagggg gcaccgcgca ggactttacc
     2761 cgtattaatc gcatgaccgc gtttagcgac gacaaataat atcagcgcgc ccacagctgc
     2821 aatggcgctc accggaatgc cgagcggttc caggacgaaa aatcccacca gcagaagcag
     2881 taaaacaacc cagccagttt tgaacgtagc aggatctttg atcgcttctg cgggagattt
     2941 cagcagcgcc atatcgtagt tctgcggaat atctttgcga aaatagagat gtaacatcac
     3001 cagcgtggca acaatcgcgg cgatatccac cggcaccatc accgaggcgt attcgcgaaa
     3061 gccgaggcca aagaaatcag cggaaacgat attcaccagg ttggagacaa taagcggcag
     3121 gctggcggta tcggcaatga atccggccgc catcacgaac gccagcgtag tgcctttact
     3181 gaaccctaaa gccagcagca tggcgatgac aatcggtgtc aaaataagcg ccgcgccatc
     3241 attggcaaac agggcggcaa cggcagcacc gagcaggaca atccaggtaa acagcaagcg
     3301 accacgacca ttaccccagc gtgagacgtg cagcgccgcc cattcaaaaa agccggactc
     3361 atccagcagc aggctgatga taatgacggc gataaacgca gccgtcgcgt tccagacgat
     3421 attccacacc accggaatat cacccggatg gaccacgccc gtaactaacg ccagtactgc
     3481 gccgagcgtt gcactccagc cgatgcctaa acctttcggc tgccagataa ccaatacgat
     3541 ggtcaggaca aagatagcgc ctgccagtaa cataatgcct cccggataaa acacatctga
     3601 aaattcatat gtgtttagct aaatttttaa ctgcaaatgt tcttactgtc cccggaacag
     3661 ttttgtcgag ccaggttgcg gacaatcgcc tgaacctttt cctgttcaca tcgccaggcc
     3721 tcatcaataa ttttcgccgc ccatgctgga atatgcggtg ataagcggta atgaacccac
     3781 ttaccttgct tgcggtccag caatagcccg ctttcacgca gcaatgccag gtggcgggag
     3841 atcttgggct gcgactggtc gagagcagtg cagagatcgc agacgcataa ctctcccagt
     3901 tcgctgagca gtaaaacgat gcccagacgg gtttcatcag caagaatttt gaacaattgg
     3961 atgggtaaca gaaatgacat attgcgctcc tgattgttgc aggtagtgtc tctcttcgaa
     4021 gcggataagt caaaaacata tatgacttaa cgaatgtgta agtgcagagg aaggtaatag
     4081 gtgtgaattt tgagttggct attcatttga aaggaggtct gaatcaggac gtaaaaaaac
     4141 cgactttgcg tcggtttttt actttccagc cctgagttgg tggctctggc tggagtgaga
     4201 gagttcttat ctaacagctc aatactatta gccagtggct aacattgcaa gaattagcga
     4261 tttcttcagc tggcggaggg ggcacttcgg tatatatcgc aatgacgatt tatatacttt
     4321 cactgggtca tcgtcatatt aagccttgtc cgaatccgga tgtaaaaaaa ccgactttgc
     4381 gtcggttttt tactttccag ccctgagttg gtggcactgg ctggagtgag aatgccatta
     4441 tctaacgcat caatgctaat accatactga aactattgca aggacatgct ggttttataa
     4501 cctgaatgta ctgtatgatt atccagttag ctctgaggca ttttcactct ggtaatgcgc
     4561 ataaacgctt tcaaagtcct ggtcagaagt acgggtggtg ccgttaactg atgctctggc
     4621 cggagtgaga atgccattat ctaacaatag ggcatgcgcc gtgacaggca gtggatgagt
     4681 aagcggatgc attctcactc catcgcatgg agaaaacggg tgattgataa agcaatcatc
     4741 gttcttgggg cgttaattgc gctgctggaa ctgatccgct ttctgcttca gcttctgaac
     4801 tgatagcgga aacgtaatta agggctaaga gcacactact cttagccctt taacatttaa
     4861 cgcattgtca cgaactcttc tgccgccgtt gggtgaatgg cgacggtatt gtcgaagtct
     4921 tttttggttg cacccatctt cagcgccacc gcgaagccct gcaacatttc gtccatacca
     4981 aagccaatgc cgtgaatacc gacaatcttc tcttccgatc caacgcacac cagcttcatg
     5041 cggcacggct ggcggtgagt ggtgacggcg gtatacatcg cggtgaaaga ggatttatac
     5101 actttcacct gatcgtcgcc atactgctcg cgcgcctgcg gttccgttaa accaacagta
     5161 ccaatcggcg gatggctgaa gaccacggtc ggaatgttgc tgtaatccag atgctcatcc
     5221 ggcttgttat taaacaggcg ttcagagaga cggcgacccg ctgcaactgc caccggtgtc
     5281 agctccactg cacccgtgtt atcgcccacc gcgtaaatac cttcaatatt ggtgttttga
     5341 tatttatcga cgacgatata gcctttttcg ttagttttaa cgccagcggc ttccaggttg
     5401 atgttgtcat tggcaggctc gcgaccaatc gcccaaatca ggcaatccac cgtttcactg
     5461 cgaccatctt ccagctccag cgtcaggcta ccatcggcat ttttcactac cgctttcggg
     5521 atggcgttgg tgtgcagctg cgggccttcg gcgttcatca cttcgaccag cgtttcggaa
     5581 atcatcgggt cgaagctgcg cagcggcgca tgtttacgca caaacagatg cgttttcgcg
     5641 ccgaggccgt taatcacgcc cgccagctca acggcgatgt aacccgcgcc aacaaccgcc
     5701 acgcgctctg gcaaagcagg aagggcgaag aagccatcag aatcaatacc gtattccacg
     5761 cccggaatat ccgggtggct cggacgaccg cctgtggcga tcagaatatg atcggccgtg
     5821 atggtttcgc cgtttacctc cagcgttttg gcatcaacga agcgggcaaa gcctttgatt
     5881 acatcaacgt tatttttacc gagcacgttt tcataggaag tatgaatacg gtcgatatag
     5941 gcggtacggc tggcgatcaa cgtttcccag ttgaatttat tgatagtggt atcaaaacca
     6001 taatccgggc cgtacatatg gatcgcttca cggatttgcg ccgcgtgcca catcactttt
     6061 ttcggcacac agccaacatt tacgcaggtg ccgcccagct ctttggcttc aatcagcgca
     6121 catttctggc cgtacatagc cgcgcggttg atggaggcga taccgccgct gccgccgccg
     6181 atggcgatgt aatcatagtg tttagtcatg acaaagtgtc cttatcgttg attaccgcga
     6241 ttgtagcgcg agacgtaata ggtgccagca atggctgcaa ttactccggc acgatccagc
     6301 ttacggtggc gtgcccggtg cctgccggaa ccagtttgct gtgcagccac ggcagcacgt
     6361 tattcatctg ctgttccagt ttccacggcg ggttaatcac aatcatgccg gaagcggtca
     6421 tgccacggcg atcgctgtct ggcagtaccg ccagttcaat ttgcagaatt ttgcgaatac
     6481 cggtcgcttc cagatcgtgg atcatgcgct taatttgctg acgcagcacc accggatacc
     6541 acagtgcgta agtaccagtg gcgaaacgtt tgtaaccttc tgctatcccg ctgaccaccg
     6601 cctgatagtc ggttttcatt tcatacggcg ggtcgataag gattaaacca cggcgggaaa
     6661 ccggcggcag tttggcctta agctgctgga aaccatcggc tttttcgacg cgcgcacggc
     6721 tgtctttctg aaattcagaa cgcagcaacg ggtaatcgct cgggtgcagt tcggtcagtt
     6781 gcaggctgtc ttgttcgcgc agtagctggc gagcaatcaa tggcgaaccg gggtagtaac
     6841 gcaactgacc gctacggttg aagtgtttta ccacattgat gtacgcctcc agttctgcgg
     6901 gcaaatcgtc ctgctgccag atacgggcga tgccttcgag atattcgccg gtacgctcgg
     6961 catgttcgct gcctaactga taacgcccgg cccctgcgtg ggtgtcgaga tagagaaacg
     7021 gtttatcttt ctctttcagc gactcgatga tcaggctctg aacggtatgt ttaaggacgt
     7081 cggcgtggtt gccagcgtga aagctgtggc gataactgag catgggtaaa ggtgttccgg
     7141 taagtaaaaa tcggcccgta ttcggggcgc acaaaagcgt atcaggacag tataccgaaa
     7201 agaggccgcc gccgcgaaag cgtaacgttt ctcattgaaa ttcactacac ttaaccccat
     7261 gctacacaca ttatgtaaag cgcctgttga gcgcttcctt aacctcttta accaggactg
     7321 cgcgaatgac gaatccgtta ctgactccct ttgaattgcc tccgttttct aaaattctcc
     7381 cggaacatgt cgttccagcc gtgactaagg cgctgaacga ctgccgcgaa aatgtggagc
     7441 gcgtagtagc gcaaggagca ccgtacacct gggaaaatct ctgccagccg ttggcggaag
     7501 tggacgatgt gctggggcgt atcttctccc cggtcagcca cctgaactcg gtgaaaaata
     7561 gcccggaact gcgtgaagcg tacgaacaaa ccctgccgct gctgtcggaa tacagcacct
     7621 gggtagggca acatgaaggg ctgtataaag cgtatcgcga cctgcgcgat ggcgatcatt
     7681 acgccacgct gaacacggct cagaaaaaag cggttgataa cgcactgcgc gacttcgaac
     7741 tctctggcat cggtctgcca atagagaaac aacaacgcta cggtgagatt gccactcgtc
     7801 tttccgagct gggcaaccag tacagcaaca acgtcctcga tgcgacgatg ggctggacca
     7861 aactcgttac cgacgaagcg gagctggcgg ggatgcctga aagcgcgctg gctgcggcaa
     7921 aagcccaggc cgaagcgaaa gagctggaag gctacctgct gacgctggat atcccaagct
     7981 acctgccggt aatgacctac tgcgacaacc aggccttgcg tgaagagatg tatcgcgctt
     8041 acagcacccg cgcctccgat caaggcccga acgccggtaa atgggataac agcaaggtga
     8101 tggaagagat cctcgctctg cgtcacgaac tggcgcaact gctgggcttt gaaaactacg
     8161 cctttaaatc ccttgccact aaaatggcag aaaacccgca gcaggtgctg gatttcttaa
     8221 ccgatctggc aaaacgcgcg cgtccgcaag gcgaaaaaga gctggcacaa ctgcgcgcct
     8281 tcgccaaagc ggaatttggc gtcgatgagt tgcagccgtg ggatatcgcg tactacagcg
     8341 aaaaacaaaa acagcacctc tacagcatca gcgacgagca gctgcgtccg tacttcccag
     8401 aaaacaaagc ggttaacggc ctgtttgaag tggtgaaacg tatttacggc atcaccgcta
     8461 aagagcgtaa agatgttgat gtctggcatc cggatgtacg tttcttcgaa ctgtatgacg
     8521 aaaataacga actgcgcggc agcttctacc tcgatctgta tgcccgtgaa aacaagcgcg
     8581 gcggggcgtg gatggatgac tgcgtaggcc agatgcgtaa agctgatggt tcgctgcaaa
     8641 aaccggtcgc gtatttgact tgtaacttca accgcccggt aaatggtaaa ccggcgctgt
     8701 tcactcacga ggaagtgatc accctgttcc acgagttcgg tcacggcctg caccatatgc
     8761 tgacccgcat cgaaaccgct ggtgtttccg gtatcagcgg tgtgccgtgg gatgcggtcg
     8821 aactgccgag tcagtttatg gaaaactggt gctgggagcc ggaggcgctg gcgtttatct
     8881 ccggtcacta tgaaaccggc gaaccgctgc cgaaagagtt gctggataaa atgctggcgg
     8941 cgaagaacta ccaggcggcg ctgtttattc tgcgtcagct ggagttcggc ctgtttgatt
     9001 tccgccttca tgccgagttc cgcccggatc agggggcaaa aatcctcgaa actctggcag
     9061 aaatcaagaa actggttgcc gtggtgccat ctccgtcctg gggccgtttc ccgcacgctt
     9121 tcagccatat tttcgccggt ggttatgcgg caggttacta cagctacctg tgggctgacg
     9181 tactggcggc agatgctttc tcgcgctttg aggaagaggg cattttcaac cgtgaaaccg
     9241 ggcagtcgtt cctcgacaac attctgagcc gtggcggttc agaagagccg atggatctgt
     9301 tcaaacgctt ccgtggtcgt gaaccgcagc tggatgcgat gctggagcat tacggcatta
     9361 agggctgatc attcagtgaa aatctgctta attgatgaaa caggcgccgg agacggcgcc
     9421 ttatctgttc tggcggcccg ctgggggctg gagcacgatg aagacaacct gatggcgctg
     9481 gtgttaacgc cggaacatct ggaattgcgc aagcgtgatg agccaaaact tggcggcata
     9541 tttgttgatt ttgttggcgg agcgatggcg caccgacgca aattcggcgg tggtcgcggt
     9601 gaggcggtgg cgaaagcggt gggcattaaa ggcgattatt tgccggatgt ggtggacgcc
     9661 accgcagggc tggggcgcga tgcctttgtg ctggcttcag tcggttgccg cgtgcggatg
     9721 ctggagcgta atccagtcgt tgccgcattg ctcgacgacg gcctggcgcg tggttatgcg
     9781 gatgcggaaa tcggcggctg gttgcaggag cgtttgcagt taattcacgc ctccagcctg
     9841 acggcgctaa ccgatattac cccacgcccg caggtggtct atctcgaccc gatgttcccg
     9901 cataagcaga aaagcgcgct ggtgaaaaaa gagatgcgtg tctttcagtc gctggtgggg
     9961 ccggatcttg atgccgatgg attgctggag cctgcacgct tgctggcgac caaacgcgtg
    10021 gtagtaaagc gcccggacta cgcgccaccg ctagcgaatg tcgccacgcc aaacgcggtt
    10081 gtgactaaag ggcatcgctt tgatatctat gcaggtacgc ccgtgtaaaa gtaaagcagg
    10141 gtagcgataa cgctaccctg ctttctgcaa gaataattaa tggctttccg gcgtcgcaat
    10201 catgcgtttc agccacggca ccatcaacag catcactact gcaacgccca gcgtgaccag
    10261 accaatctta ccaaacacgt tggtatagac gggcaacgtc tcaagcggat cggtaatgtt
    10321 gtccggcacc gcggtaaatg ttgccacata gccgcccagc aagaacgcgg cagcctgcgt
    10381 caggaaccac atcccgagaa taaagcccat caaatgctgc ggcaccaggg cagcaatcat
    10441 cgccaggcca agggcgctaa taaacagttc acctaagctc tggaataagt acaccagcac
    10501 gataaaccat ggcgatgtca gcccttgtgc atccgcaaac cacattcccg cagctgccgc
    10561 cgtcaaaaag cccagtgagc acataaacat gccgagagta aatttcatcg gcatcgagag
    10621 gtctttgcct ttgttaccca gatgcgtgta aatgcctgcc agtattgggc tggcgagtac
    10681 cacccagaac gggttaagcg cctggaagct gaccgggttg atggaaaaac cgagaatttc
    10741 atgatgcacg ttgttgatgg caaagaagtt cagcgatgtt ggcatctggg cgtagagaat
    10801 gtaaaacacc accgcttcga gcatcaggac aaaggcgaca aacattttat tgcgcccggt
    10861 tttatccagc ttgaatgcct gacgaaagaa gatgatggtg acgacgatgg agagaacaat
    10921 cagcaccaga ttggcgactt ctacgttgtg catcagccat gcacatacga agatcatcac
    10981 cacgctgcca agtaatacgt acaacagttt gctgaagctc atcggcttga agtcgggttc
    11041 agaaccaatg tctttcacca ttccacgaca ggcgatgtaa accagtaatg cgataattaa
    11101 ccccgccccg cacaggttgt aggtgactga ataaccgaat ctatcagcga tcacaggggc
    11161 cagcgataac gctatcaacg agccgatatt gatcgacata tagaacaggg tgaatgcgcc
    11221 atcaagccgc ggatctttcg gcggatagca cttcgaaagc aagctggctg ggttagcttt
    11281 aaacaggccg ttaccgacag cgatagtccc cagggcgatg aaaatcaggt caggcttaag
    11341 tagcgacatg ccggtcatga agtagccaat cgccagcaca agtgctccga gaacaatggt
    11401 gcgtttggtc cccagcaggt ggtcgccgac atagccgcca atggaaatga ggccatagac
    11461 cagcgcagca aaagcaccaa aagtgacaaa agcctgctct tgcgagaatc caagctgttt
    11521 aacgaagaaa accgccagta cgccctgcac gccgtagtag ccgaatcgct cccataactc
    11581 gacaaaaaag atcatgaaaa atgggcgagg ttgctgcagc atccccatgg gtgttgttgt
    11641 attcatattt attaaccttc caataccatc ctgaaaagta tgaacgcgta agggataacg
    11701 cgcgaaaaag tttacatctg tcagagattc ttaatattga tgcggtgaat agattaatac
    11761 actattgcaa cagaaaataa ccagctgatc acactaatga tggaaaatat gatggtgtta
    11821 aatgttgtat tggcaggaga ttttgattgc gtacgcagat cgtaggccag ataaggggtt
    11881 tacgctgcat caggtaacaa ggcgcgccaa aaagcaaaac gcccgcatat gcgggcgttc
    11941 aggaacacgt cgtggaggga tgattattct tcttcgtcgc gcagcggaac aatcagcata
    12001 tcaacgtgaa cggtgttgat cagctgacgt gcggaagaca tcagtttgct ccagaagtcc
    12061 tggtggtgac cacaaaccac cagatccata tcgtatttct tgattgcatc gaccagaacc
    12121 tggcccaggt cgccgctgcc gctcagggtt tcagtgattg ggtagcctgc attagtggaa
    12181 agctcggtca gcgcatgatg tgtctcttca gagatgcgtt tctgcatatc acccagattc
    12241 acatcaataa gcccggtgta taggtcagag tagtttacat ctacgtggat cagagaaact
    12301 ttcgcattgt aggggcgagc catagagact gctttctcta ccagaacttt gctttccggg
    12361 gagagatcga ctgcgatgag aatgtgttta taagccatag tgttactcct tccataaagt
    12421 tgtcgatgac tggccagcta gcgtttcttg tgccgttacg tcacccgcgt cctgcgaact
    12481 acgtgcgcgg ggctaaccca gccgtaaaga ttcagtgtta agaccatcct taccttatag
    12541 cgacccggat gatccgtcaa tccgccttgc ttacccgtta gataaacaaa ttttcgacat
    12601 gaatatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
    12661 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
    12721 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
    12781 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
    12841 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
    12901 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
    12961 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
    13021 aatttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgaccagc gcattgtgtg
    13081 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
    13141 aacgcataaa aaaagcgggc cagtaaactg acccgctcga gactgattac aaacgcaaaa
    13201 ttgcctgatg cgctacgctt atcaggatct acataatctc tgcaatctat tgaatttgca
    13261 tgtttttgta ggccggataa ggcgttcacg ccgcatccgg catgaacaaa gcgcactatg
    13321 tcacaatctg aagcgggcca gctgacccgc tcgttttggt gtgtacgatt acaggaactg
    13381 caaggagagc cagtacagtc cgccggaaag cagtaccgca gccggaaggg taaacaccca
    13441 ggccatcaga atactggtta cggttttacg ctgtaagccg ccgccatcta ccaccatcgt
    13501 ccccgcgaca gaagaggaga gtacgtgagt agtggaaacc ggcatcccgg tataactcgc
    13561 caggccgata gacactgccg ccgtcatctg ggccgacatc ccctgagcgt aggtcatgcc
    13621 tttcttaccg attttctcac cgatagtcgt tgccacacgg cgccagccaa tcatcgtacc
    13681 gatacctaac gccagcgcga ccgccatgat gatccacacc ggtgcatact cgatggtgct
    13741 aagcatgtcg gacttcagtt tcttcaacag gcgctgatcg tcagcactca caccaggcat
    13801 cttcaccact ttgtcgatag tgtcagaaac gcacagcata atgcggcgca tctggctacg
    13861 ttgatcaagc gacagcttgt cgtagctttc cacatcggta gtcaacatgc ctttcaggcg
    13921 gttgagcgcg ttaatggtat tcgacggatg gcaatggaac tccgcgggtt gcgttgcgcc
    13981 agcttccgga gccggtacta actgatcagc accggtagcc tgtttgagca gcgcaggatg
    14041 ctgctcaaag taagcttcga cgttgttgat ggcatcacgg gtacgggtga tttcgtagcc
    14101 agtggcattc atgttcacca cgaagcctgc tggtgcgacg ccaatcaata ccaacataac
    14161 cagaccaatg cctttctgac catcgttcgc gccgtgcgaa aacgccacgc cgatagcgga
    14221 aaggatcagc gcaatacgcg tccagaacgg cggctttttc ttgccgtctt tcttttcacg
    14281 ctccgctggg gtcaggtgga tacgggcgcg tttcttggtg ccgctccagt agcgacgcag
    14341 caagaaaatc agaccgccag caaacaccag gccgacaata ggggaaacga tcagagaacc
    14401 gaaaatactt aatactttcg ggatattgag tgcatccacc actgacgtcc cggtcatcaa
    14461 cgcattggtt aaaccaatcc cgatgatcgc gccaatcagc gtatgagagc tggatgcagg
    14521 taaaccaaag taccaggtac ccaggttcca gataatcgcc gccagcaaca tagagaacac
    14581 catggcaagg ccatgagacg atcccatatt aagcagcaga tccgtcggca gcatatgcac
    14641 aatggcatag gcaacactca gaccacccag caaaacaccc aaaaagttga acaccgctgc
    14701 cataaccacg gcgagctgag aacgcatcgc gcgggtatag ataacggttg ccacggcgtt
    14761 ggctgtgtca tggaaaccat tgatggcttc gtagaacagc acaaaagcca gtgcaagcaa
    14821 taataacagc ccggtatgca aatccaggcc agcaaacaaa tgtagcatag gacgttacgc
    14881 cattttgagg acatgaacgc ggcgcattat cagtgacttt cgcggcgcgg gcaaagtgaa
    14941 atatagactt tttttgattt gcctcctgta tggatttcac tcaaaaaata attatcttat
    15001 ataattcagg caaatacttc cttttagtaa tattgatgct ggtgcgacca ctgaggaatc
    15061 tttacaattc acgcccgttt tttctaagag gagcgcaacg tggaaaggtt tgatgccatt
    15121 attataggcg ctggtgcggc gggtatgttc tgttctgcgc tggcaggtca ggcaggacgc
    15181 cgggttctgc tgatcgataa tggtaaaaaa ccagggcgca aaatccttat gtctggcggt
    15241 gggcgctgca actttaccaa cctttatgtc gaaccaggcg cttatctgag ccagaatccg
    15301 catttttgta agtctgcact cgcgcgtttt acccagtggg atttcattga tctggtcaat
    15361 aaacacggca tcgcctggca cgagaaaacg ttaggacaac tcttctgcga tgactccgcg
    15421 cagcagattg tcgacatgct ggtggatgag tgcgagaagg gcaatgtaac cttcagattg
    15481 cgtagcgaag tgctgagtgt ggcgaaggat gaaacaggct tcacgcttga actgaacggc
    15541 atgactgtcg gttgcgaaaa gctggtcatc gcgaccggtg ggctgtcaat gccggggctg
    15601 ggcgcgtcgc cgtttggtta taagattgcc gaacaatttg gcctcaacgt gctgccgacc
    15661 cgcgcgggtc tggtgccatt cactctgcat aaaccgttgc tcgaagagtt acaggtgctg
    15721 gcgggcgtgg cggtgccttc cgtgattacc gctgaaaacg gcatcgtttt ccgtgagaac
    15781 ttactcttca cccatcgcgg cttgtctgga ccggcggtgt tgcagatttc cagctactgg
    15841 caaccgggtg aatttgtcag tatcaatctg ctaccggatg tggatctcga aaccttcctg
    15901 aatgagcagc gtaacgcaca tccgaatcag agcctgaaaa acacactggc ggttcatcta
    15961 ccgaagcggt tggttgaacg cttacagcaa ctcgggcaaa tcccggatgt ttcgctaaaa
    16021 cagctcaacg tgcgtgacca acaggcactg attagcacat tgaccgactg gcgcgtacaa
    16081 cccaacggca ctgaaggcta tcgcactgcc gaagtgacgc tcggcggcgt ggacaccaac
    16141 gaactctctt cacggacgat ggaagcgcgc aaagtgcctg ggctgtactt catcggcgaa
    16201 gtgatggacg ttaccggctg gctggggggc tataacttcc agtgggcgtg gtcgagtgcg
    16261 tgggcttgtg cgcaggattt gattgcagcg aagtcgtcct gacctgttac ctaaagatga
    16321 aaattattag agccgcatta aaaatgagct aattttgata gtggctatct tgtgattatt
    16381 ttctaatgag cccgtgaact gaaaccctcc aggcttaata taaggtggaa ggaaaggtga
    16441 ttgaaaactc acccagtggc cagatcttta taaaatatga acaggttgaa aaaaacagta
    16501 actttgttgt ttttttatcc ttaaccacta tgcattaatg ctgcgttatt tcatgatgcc
    16561 taagaaaaac cagcgttacg caaatggtca acgctggttt tatccggtac gttgcaatta
    16621 ttttttagca gaacctgctt ctaatattga aacgattgag aacaacgtaa agcataccgc
    16681 tcctaaaccg accaaaaccc gcgaaggaat aaaatatccc atgtcggtct gcgccatttc
    16741 cgcaagaaac gatgccagga aaaggcaaaa caggcaggtg aagacgggaa tcatcggtat
    16801 tctgttggct aacgaacagg tacgtctcca taccagtgcc agtagccaga cttttgagaa
    16861 tatgctgtaa cagatcattc cgaggcaaat aagaataatg ccgggagcca gtcgggcgct
    16921 tgcatcggaa ctgactaaga cgtatatccc ctgcagtacc gtgattgagc cgagaaaaat
    16981 aacccaataa caccacagcc agtgttcttt agttgaaaac gtattacgtg tttgatggac
    17041 aattgtggca acaaggccaa ttagacaggc acagattgcg gttaacccaa gcaatacgtg
    17101 acccgcgaca taatgcggag ttatgtcagc actacgtaac agcgtaatag accaaatgaa
    17161 accgagcagg gtgagtaaaa cgggaacggt aatgaggcaa ttaccgatta atgaagaata
    17221 agcctgtacc ggcgttccat cgctcttgct ccccgctgca tttttgggaa tgagcagaaa
    17281 gtgaccggat gacgctgcca ccgtcgatac acaggcggca atcataccaa cgccgaaaat
    17341 aacatggccg gcgacaaact cgtctgccat cacatcattg cctgctaaca gtgcccaacc
    17401 ccatatcatg gtgataattg accccgcata gccaataatg gggaacaatg tattgtaaaa
    17461 cgtattaacg ccgcgcgtga gctgcgaaat gataataaat gcggtagtga ataatgccag
    17521 gcatatggct gccagagaaa tcagcacatg acccgcaacg aaatactcac tttgcccaga
    17581 actttccagc acaaagccac ctaacgcgat acaaattaag cccataatta aagggattaa
    17641 tttgaaaagc cacccgatat atatgttcac tttaaactcc tcaggtatcc ctgctaatat
    17701 tgaaatgtgc attaatgata atttcagcct gttaatataa tgacgagata tcattaaaga
    17761 caaatgcata gcataactat agcgggattt actttgcaga gcgctacttt tcagcgttca
    17821 aaaggccatg gtaaataaat atttatccta agattaattc ttattctgtt gttttttaca
    17881 attatcttcc agggagggat tctttttcat tatttggtaa aagaccggat ggtatcggtg
    17941 cttaaaatca gcgaacatat aagtattatt gttttatatc gtcgaaaata tgtctttcag
    18001 acatgtatta tttattttgt tactaacaag tcaaaaatat cgcagg
//
LOCUS       DAZFWL020000091        17873 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFWL020000091 DAZFWL020000000
VERSION     DAZFWL020000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.091"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..137)
                     /locus_tag="ACHKNP_RS16535"
                     /old_locus_tag="pgaptmp_003307"
     CDS             complement(<1..137)
                     /locus_tag="ACHKNP_RS16535"
                     /old_locus_tag="pgaptmp_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HGU4705423.1"
                     /translation="MAQAAIFKEIFDQVRKDLNCELFYSELKRHNVSLYIYYLATDNI
                     H"
     gene            438..644
                     /locus_tag="ACHKNP_RS16540"
                     /old_locus_tag="pgaptmp_003308"
     CDS             438..644
                     /locus_tag="ACHKNP_RS16540"
                     /old_locus_tag="pgaptmp_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705424.1"
                     /translation="MNKEQSADEVSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            1217..1744
                     /locus_tag="ACHKNP_RS16545"
                     /old_locus_tag="pgaptmp_003309"
     CDS             1217..1744
                     /locus_tag="ACHKNP_RS16545"
                     /old_locus_tag="pgaptmp_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HGU4705425.1"
                     /translation="MSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPVAATV
                     RGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQRKLI
                     PVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQEKSYE
                     ACAAELIPDEEGESL"
     gene            1753..3852
                     /locus_tag="ACHKNP_RS16550"
                     /old_locus_tag="pgaptmp_003310"
     CDS             1753..3852
                     /locus_tag="ACHKNP_RS16550"
                     /old_locus_tag="pgaptmp_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU4705426.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWSCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            3849..4061
                     /locus_tag="ACHKNP_RS16555"
                     /old_locus_tag="pgaptmp_003311"
     CDS             3849..4061
                     /locus_tag="ACHKNP_RS16555"
                     /old_locus_tag="pgaptmp_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705427.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            4061..5569
                     /locus_tag="ACHKNP_RS16560"
                     /old_locus_tag="pgaptmp_003312"
     CDS             4061..5569
                     /locus_tag="ACHKNP_RS16560"
                     /old_locus_tag="pgaptmp_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4705428.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLAP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGRKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            5514..7541
                     /locus_tag="ACHKNP_RS16565"
                     /old_locus_tag="pgaptmp_003313"
     CDS             5514..7541
                     /locus_tag="ACHKNP_RS16565"
                     /old_locus_tag="pgaptmp_003313"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGU4705429.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQSLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            7628..7951
                     /locus_tag="ACHKNP_RS16570"
                     /old_locus_tag="pgaptmp_003314"
     CDS             7628..7951
                     /locus_tag="ACHKNP_RS16570"
                     /old_locus_tag="pgaptmp_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HGU4705430.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGITEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSEPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            7944..8219
                     /locus_tag="ACHKNP_RS16575"
                     /old_locus_tag="pgaptmp_003315"
     CDS             7944..8219
                     /locus_tag="ACHKNP_RS16575"
                     /old_locus_tag="pgaptmp_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HGU4705431.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMIVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            8231..8809
                     /locus_tag="ACHKNP_RS16580"
                     /old_locus_tag="pgaptmp_003316"
     CDS             8231..8809
                     /locus_tag="ACHKNP_RS16580"
                     /old_locus_tag="pgaptmp_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4705432.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTVRIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGSLTQ
                     AFEDARDHIIAAEMPKQLGYALKQQLRLWLTR"
     gene            8806..9207
                     /locus_tag="ACHKNP_RS16585"
                     /old_locus_tag="pgaptmp_003317"
     CDS             8806..9207
                     /locus_tag="ACHKNP_RS16585"
                     /old_locus_tag="pgaptmp_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4705433.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            9218..9961
                     /locus_tag="ACHKNP_RS16590"
                     /old_locus_tag="pgaptmp_003318"
     CDS             9218..9961
                     /locus_tag="ACHKNP_RS16590"
                     /old_locus_tag="pgaptmp_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4705434.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVSPASASVKVGATTTLTFTVKPDGASDKAISVHSSDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVTAVTVSAAG"
     gene            10022..10408
                     /locus_tag="ACHKNP_RS16595"
                     /old_locus_tag="pgaptmp_003319"
     CDS             10022..10408
                     /locus_tag="ACHKNP_RS16595"
                     /old_locus_tag="pgaptmp_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4705435.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAVDDEHEALTPEKS"
     gene            10417..10746
                     /locus_tag="ACHKNP_RS16600"
                     /old_locus_tag="pgaptmp_003320"
     CDS             10417..10746
                     /locus_tag="ACHKNP_RS16600"
                     /old_locus_tag="pgaptmp_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4705436.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10718..13783
                     /locus_tag="ACHKNP_RS16605"
                     /old_locus_tag="pgaptmp_003321"
     CDS             10718..13783
                     /locus_tag="ACHKNP_RS16605"
                     /old_locus_tag="pgaptmp_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4705437.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRLNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARVAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            13783..14112
                     /locus_tag="ACHKNP_RS16610"
                     /old_locus_tag="pgaptmp_003322"
     CDS             13783..14112
                     /locus_tag="ACHKNP_RS16610"
                     /old_locus_tag="pgaptmp_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4705438.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCDHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            14122..14820
                     /locus_tag="ACHKNP_RS16615"
                     /old_locus_tag="pgaptmp_003323"
     CDS             14122..14820
                     /locus_tag="ACHKNP_RS16615"
                     /old_locus_tag="pgaptmp_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4705439.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVIWQGRQYQVYPIQGTGFELNGKGSAARPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14825..15568
                     /locus_tag="ACHKNP_RS16620"
                     /old_locus_tag="pgaptmp_003324"
     CDS             14825..15568
                     /locus_tag="ACHKNP_RS16620"
                     /old_locus_tag="pgaptmp_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4705440.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNLEAT
                     GLYQVALSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            15466..16113
                     /locus_tag="ACHKNP_RS16625"
                     /old_locus_tag="pgaptmp_003325"
     CDS             15466..16113
                     /locus_tag="ACHKNP_RS16625"
                     /old_locus_tag="pgaptmp_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4705441.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGSEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16174..>17873
                     /locus_tag="ACHKNP_RS16630"
                     /old_locus_tag="pgaptmp_003326"
     CDS             16174..>17873
                     /locus_tag="ACHKNP_RS16630"
                     /old_locus_tag="pgaptmp_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU4705442.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQNRPSDKAWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPEGVAEYSVWGLKLPTL
                     R"
BASE COUNT         4292 a         4161 c         5405 g         4015 t
ORIGIN      
        1 atgtgaatat tatctgtggc taaatagtaa atataaagtg agacattgtg acgttttagc
       61 tcagaataaa ataattcaca gtttaaatct ttacgcactt gatcgaatat ttctttaaaa
      121 atggcagcct gagccattgg taaaccttcc atgtgatacg atggcgcgta gttagcatta
      181 tcgtgtttat tgtttcaatc tggtctgacc tctttgtgtt ttgttgatga tttatgtcaa
      241 atattaagcc tgtttttaat gaatagtgtt gattgcgtaa caaaattgag ccttgctggc
      301 atccaggagg gatatgcaac cgacagatgt atgtaaggtc gatgtactca aactttcata
      361 cttttcctct tttatgcagt aagatttgaa gtaatatttt aaccgctaga tgacgagcaa
      421 acgcatggag cgacaaaatg aataaagaac aatctgctga tgaagtctcg ttggatctga
      481 ttcgtgtaaa aaatatgctt aatagcacca tttctatgag ttacccggat gttgtaattg
      541 catgtataga acatcaggtg tctctggaag cattcagggc aattgaggca gcgttggtga
      601 agcacgataa gaattcgaag gattattccc tggtggttga ctgagcacca taactgctaa
      661 tcattcaaac tatttcacct gtgacagagt caatatcgca ttctgtcact gtcaggctaa
      721 tacagagctg caattcaact actgcaatgt cctcgtaatt aggtgaattt acaatatcgt
      781 cctgttcgga tgccggttgc attgctgaag atgaggcatt tatggttcgc atattttccc
      841 ctcatgctcg tcagtcctgt gcgtaggaag aaacaggaca ctcacactaa tttgtgtggg
      901 catgctgtga tgtccttctg aattattcct atgccattat gtaaagcgct gtatcagatg
      961 ctcatcacgg ctgtcaggct gtcaggctgt cgggtcctcc cggtgggggc ccctgccacg
     1021 gggcgggagc gtcgcggaaa aaggctagtt tttgcatttc catggcggcg gcagcatgtt
     1081 tggtaattta ttgataatta aaagttattt ctcttttcac ctgtacaata tttctttctc
     1141 cctgtcatta gaccagtttg caattaattg aaatatataa ataaacatga ttttcacctg
     1201 ccagatggag ttgcttatgt caaatgtgag cgggatcggt gatgcttatt actggagtgt
     1261 ttttaaaatc gctgaggcct ttgggcttca ccgggacaca gtaaaaaaac ggctcctcgc
     1321 ggctaacact cctgtggctg cgactgtcag ggggaacccc gtttacgccc tgcagcatgt
     1381 cgggccagcc ctgtttagtg tgaagcatga ggcagcagac tctgttcatg atccatcccg
     1441 tatggagccg aaagagagaa aggactggta ccaatctgaa aatgaaagga tcaagctgga
     1501 aaaggagcag cgaaaactca tccccgttga tgaagtagtc atcgtctatt cgtccatgag
     1561 aaaggctgtc gtccaggttc tggagacaat tccggatgtt cttgaacgcg attgcgccct
     1621 gactcctcag gccgtcggcg ttgtacagca ggccattgat gacctgcgat acactcttca
     1681 ggaaaaatcc tacgaggctt gtgctgctga attaattcct gatgaggaag gagagagtct
     1741 ctaggaggaa taatgggttt ttcatcagcc cgaaatttgg gaagggacat atcggcagga
     1801 ttttccccac cacgtcgcat gccgatttcg gaggctgtta aaaaattcat gcgtgttccc
     1861 aagggggctg gtaactcggt gccatgggat cctgaactga caccctacat cattgagccc
     1921 atgaactgcc tggcatcgcg tgaatacgat gcggtgattt ttgttggtcc tgcgcgaaca
     1981 gggaagacca ttggtctgat cgatggatgg attgtctata ccatcgtttg cgatccttcg
     2041 gacatgctcg ttgtgcagat gaccgaagat aaggcccgcg agcattctaa aaagcgcctc
     2101 gacagaacgt tcagaagcag tgcggcggta aagaaaagaa tgagtccacg tcgtaacgac
     2161 aataatgtcc atgataagac gttcagggat ggctcgttcc ttaaaattgg ttggccctcg
     2221 gtcaacatta tgtcgtcgtc ggattaccgg tttgtcgcct taaccgatta cgaccgtttt
     2281 ccggagaata tcgacagcga gggtgatggt ttctccctgg cctcaaaacg taccaccaca
     2341 tttatgtccg ctgggatgac tctggtggag agctcgccgg gacgtgacat ctgcgacagc
     2401 aaatggcgac gtaagtcgcc tcatgaagcg ccaccgacga ctggtattct ttccctttac
     2461 aatcgtggtg accgccgccg ctggtactgg tcatgtccgc actgcggtga atattttcag
     2521 ccagccatgg atgccatgac cggctaccgt aatgaaccgg atccctttaa agccagtgag
     2581 gcggcgtatc tactttgccc gcactgcagc ggcattatca ctgcggagaa aaagcgtgag
     2641 ctcaatagtg caggagtctg gttgcgtgaa ggtcaggtca ttgatcgtaa cggcaacgtt
     2701 tccggtgaac cgcgccgctc ccgtatcgcc agtttctgga tggaagggcc agctgctgcg
     2761 tatcagacct gggcgcaact ggtttacaaa ttactgactg cagaacagga gtatgaagcg
     2821 acaggaagcg aagaaacact cagggcggtt atcaacaccg actggggatt gccttatctt
     2881 cctcgtgcca gcatggagca acgaaaaagt gaactgcttg agcagcgggc agagccagtt
     2941 ccttcccgca gtgtgccgga tggcgttaat ttccttgtgg cgacagtgga tgtgcaggcg
     3001 ggacgtcatc gccgttttgt ggttcaggta acgggctatg gcagtcgtgg cgaacgctgg
     3061 attattgatc gttacaacat cacgcagtca ttgcgcagtg actgcgacgg ggagagccag
     3121 cgaattgatc cggccagcta tccggaagac tgggatgtcc tgctgacgga tgtttttcat
     3181 aaaagctggc cgctggcctc cgatccttct caacaaatgc gactgatggc aatggcggtg
     3241 gactccggcg gtgaagacgg ggtcactgat aatgcctata aattctggcg tcgttgccgt
     3301 cgtgatggcc ttggtaaacg tatttacctg tttaagggtg acagcatccg gcgcgcaaaa
     3361 ctgatcagcc gtacattccc tgataacacc ggacgaacgg gccgccgggc gcaggccgca
     3421 ggtgatgtgc cgctctggct tcttcagacg gatgccctga aagaccgggt gaataacgcg
     3481 ttatggcgtg actcgccagg tccaggctat gtgcatttcc ctgactggct ggggagctgg
     3541 ttttacgacg aactgacgta tgaagagcgg agcagtgacg ggaaatggag taagccgggt
     3601 cgcggtgcca acgaagcttt tgacctgatg gtgtatgccg aggctctggt cattctgcat
     3661 ggatacgaaa agatccgctg gccggatgca ccggagtggg cgagccggga gacctggctg
     3721 gagtgtgtcc aggacagtac cgaaccgtca tcctcaccgg aaccggtatc cacgcctgtt
     3781 aaaaaacaaa aacggaagaa aacagtaact gacgatgtta acccctggct gacttccgga
     3841 ggatggttat gaaccagaat gatatcgaag ccatgattca gcgttatacg gaagctgaaa
     3901 tggcggtgct ggacggaaaa tccgtcacct ttaatggtca gcagatgacc atggaaaact
     3961 tatctgagat ccggcaggga cggcaggagt gggagcgccg ccttgcggct ctgattacac
     4021 gacgacgggg gcatcccggg taccggctgg cgaggttctg atggcaattc ttgatgatgt
     4081 gattggcgtt ttttcaccag gatggaaagc ggcaaggctg cgttcccgtg cggtgatcca
     4141 ggcttatgag gccgtaaaaa cgacgcggac acacaaagcc cgacgggaga accgaactgc
     4201 cgaccagtta agccagtacg gggccgtgtc gttacgtgag caggcccgtt accttgataa
     4261 caaccacgat ctggttattg gtgtatttga caagctggaa gaacgggtgg tggggaaaaa
     4321 cgggattatt gtcgagccac atccggtatt acgcaatggg gccattgccc gtgacctggc
     4381 tgcggagatt cgcacccgat ggagtgaatg gtctgtcagc ccggaagtca ccgggcagtt
     4441 tacccgtccg atgctggaac gtctgatgct tcgtacctgg ctgcgcgatg gtgaggtgtt
     4501 tgcccagatg gtttccgggc gcataaacag cctggcgcct tctgccggtg ttcatttctg
     4561 gctggaggcg ctcgagccgg actttattcc catgactagt gatgagagca acaggctgaa
     4621 tcagggcgtg tttgttgatg actgggggcg tcccgaaaaa tatctggtgt ataaaagccg
     4681 tcccgtatcc ggacggcaga tggaaaccaa agaagtggat gcagagcgaa tgctgcatct
     4741 taaatttgtt cgccgtctgc accagatgcg cgggacgtct ttgttgtccg gtgtgctgat
     4801 ccgcctcagt gccctgaaag aatatgaaga ttctgagctg actgcagcaa ggatcgccgc
     4861 tgctctgggg atgtacatcc ggaaaggcga cgggcagagc tatgaaccgg atggtaatgg
     4921 cagaaaggag aatgaacgtg agcttaccat tcagccgggc attatttacg acgatctgaa
     4981 acccggcgaa gaaatcggaa tggtgaagtc ggatcgcccc aatcctaacc ttgaaacttt
     5041 tcgtaatggt cagttgcgtg ccgtggcggc gggcagtcgt ctgagttttt ccagtacagc
     5101 gcgcaactat aacggcactt acagcgccca gcgtcaggag ctggttgaat ccactgatgg
     5161 ctacctgatc ctgcaggact ggtttattgg tgccgtcacc cgcccgatgt atcgtgcatg
     5221 gctgaaacag gctgtggcat ccggtgttat caggctaccc cgcgatcttg accgttcttc
     5281 actgtatacc gcggtgtatt ccggaccagt gatgccgtgg attgaccctg ttaaggaggc
     5341 tgaggcctgg aaaatccaga ttcgtggtgg agcggcgaca gaatcagact gggtacgtgc
     5401 tggtggtcgt aatccggatg atgtcaaacg tcggcgcaag gccgaaattg atgaaaaccg
     5461 caagctggat ctggtatttg ataccgatcc ggccagtgat aaaggaggca gtagtgccgc
     5521 aacgaaacga caggagccgc agcacaccga cgaccagtcc gaagaataat tcctggttca
     5581 ggatgcaggc tggtcaccag agtgacgcgg atatttatat ttatgacgag attggtttct
     5641 ggggtgttac agcgaagcag tttatcagtg atctgaatgc actgggcgat atcacccaca
     5701 ttaatctcca tattaattca ccgggtggcg atgtctttga aggcatcgcc atttttaatg
     5761 cgctgaaaac acatggtgcg tccattaccg tttatgtcga cggtgtggcg gcgtcaatgg
     5821 cgtcggtcat tgcgatggta ggaaacccgg tcattatgcc ggaaaactcc ttcatgatga
     5881 ttcataaacc atttggcttt acgggcggtg atgcggagga catgcgcacc tatgccgacc
     5941 tgctcgataa agttgaggcg gttctgttac ccgcttatgc acagaaaacc gggaaaacca
     6001 ccgatgaaat tgctgccatg ctggcggatg agacctggat gtccggtgcc gaatgtctgg
     6061 cacagggatt tgctgatcag gtgacaccag ccgttaaggc aatggcatgt attcagtcaa
     6121 aacgtacaga ggaatttaaa aagatgccgg aatccattcg aaatatgatt actccgccac
     6181 gcaacagtgc tccacgcgta caggatgatg gacctgcagc ctcccggacg ccagtgcagg
     6241 cagcagcacc cgtggtggat gaaaacagca tccgtgcgca ggtcctggca gagcaaaaag
     6301 cgcgtgtaaa cggtattaat gatctgtttg ccatgtttgg cgggcgttat cagtcgttgc
     6361 aggctcagtg tcttgccgat cctgaatgtt cgctggagca ggcccgcgaa aagctgttga
     6421 acgagatggg gcgcgagtcc acgccatcca ataaaaatac cccggctcat atttatgccg
     6481 gtaacggtaa ttttgtgggg gacgggatcc gccaggcgct gatggcgcgc gccgggtttg
     6541 aaaaaactga acgtgataat gtctacaacg ggatgaccct gcgtgaatat gcccgtatgt
     6601 cactgactga acgtggtatt ggtgtttcca gttataaccc gatgcagatg gtcggtgcgg
     6661 cgttcacaca cagtacgtct gacttcggta atattctgct ggatgttgcg aacaaagcca
     6721 ttctgcaggg ctgggaagat gcccctgaaa cctatgaaca gtggacgcgg aaaggtcagt
     6781 tgtctgattt taaaattgcc catcgtgtgg gtatgggggg cttcagtgct ctgcgtcagg
     6841 tgcgtgaagg ggcagaatat aaatacgtca ccaccggaga taaacaggcc actattgcac
     6901 tggcgaccta tggcgagctg ttcagtatca cccgtcaggc cattatcaat gatgatctga
     6961 atatgctgac cgatgtcccg atgaagctgg gccgtgcggc gaaatccact attgccgatc
     7021 tggtttatgc cattctgacg tctaacccga aaatctccac agataatgta agtctgttcg
     7081 ataaagcgaa acatgcaaac gtactggaga gcgcggcaat ggacgtggca tcgctggata
     7141 aagcccgcca gttgatgcgc gttcagaaag agggtgagcg tcatctgaat attcgtcctg
     7201 cgttcgtact ggtaccgacg gcgatggagt ctgttgctaa ccaggtcatt cgctcctcaa
     7261 gtgtcaaggg ggctgacatt aacgccggta ttattaaccc ggtgaaagat tttgcgaccg
     7321 ttattgcaga gcctcgtctt gatgataaca gccagaccac cttctacctg gctgcgtcca
     7381 aaggctccga tacgattgaa gtggcttatc tcaacggtgt ggatacgcca tatattgatc
     7441 agatggaggg cttcagtgtg gatggcgtga caacgaaagt gcgtattgac gctggtgttg
     7501 cgccagttga tcaccgcggc ctggtgaaat gtacggcgta aacgtcgcag acaacaactc
     7561 tgatggcccg taagggcttt ttttgtacct gaaatcagcc cctgaacggg gctgtgcgga
     7621 gacagttatg gcaaagaatt ttgtagaaga aggaaaaacg gtggcgattg ttgccagtgc
     7681 agccatcagc agcggagatc tggtgcaggt gggggatgtt tttgcggtgg cgctgaccga
     7741 tattccacag ggagaaacag gcgacggcat aaccgaaggt gtgtttatgc tgcctaagct
     7801 gaaaacggat gacatgaaaa cgggtaagaa ggtttatctg aagtcaggaa aagttcagct
     7861 gactaacagc ggctctgagc cgctggtcgg ggttgtctgg gcagatgccg gaaccagtgc
     7921 agaagaagtg ccggtaaaac tcaatgtctg atcccttttc ccggctggca gcgcgtatgg
     7981 atgcgatcac ggtcagaaag atgggaaaga cagcctcgat taatgatgtc gatatgattg
     8041 tgatcccggg agaaacactg gcagagctga atgctctgtc cggacctgcg gtctctctgg
     8101 tggtgttttc ttcgggatac cgcccacggc gcggggatcg cgttgtttat gacggacaac
     8161 aatggacggt cacacggcat gaacgtttta acggtaagcc aatgatcttt attgagtaaa
     8221 gaggtgtggg atgaaggggc ttgagaatgc catccgcaat ctgaacagcc ttgatacccg
     8281 tatggtgcca caggccagcg catgggcgat aaaccgtgtg gcacagaaag cggtctcggt
     8341 tgccacccgg caggttgccg ggaataccgt tgcgggagat aaccaggtga aagggatccc
     8401 cctgaaactg gtacgtcagc gtgtccgggt gtttaaagcc agtccgtcag gaaaaatgac
     8461 ggtcaggatc cgcgttaacc ggggcaatct gcccgccatt aagctgggga cagcccgggt
     8521 cagactggcc cggcgtggtg gaaaactgca gtaccgtggc agtgtgctga aggtgggtaa
     8581 atatcttttc cgggatgcgt ttattcagca actggcgaat ggtcgctggc atgtgatgcg
     8641 acgtattgat ggcaaaaatc gttaccccat tgatgtggtg aaaatcccgc tgtccggatc
     8701 gctgacacag gcatttgaag atgcccgcga ccacatcatt gctgcggaaa tgccgaaaca
     8761 gctggggtat gcactaaaac aacaactgag gttatggctg acccgatgaa ccgacataca
     8821 caaatccgcc aggccgtact ggcacgcctt cgggaacagt gtggagacag cgccacgttt
     8881 tttgacgggc ttccggcatt tattgatgcg caggaactgc ctgccgtggc ggtgtggctg
     8941 agtgatgctc agtacaccgg aaaaatgacg gatgaagatg actggcaggc tgttctgcat
     9001 attgccgtct tcatccgggc acaggcaccg gattcagagc tggatatgtg gatggagagc
     9061 accattttcc ctgccctgaa tgatataccg gcactttccg gactcatcga caccctgatc
     9121 ccactcggtt ttaactatca acgtgataat gagatggcca cctgggcgat ggcggaaatc
     9181 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
     9241 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
     9301 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
     9361 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
     9421 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
     9481 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
     9541 tcgtatcaaa tatcccaacg gcactgttga tgttttccgt ggctggctga gctcactggg
     9601 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
     9661 tccgtatctg gcagaggaag gcactgaaac cgtgagcgtt accgggctga cagtatcacc
     9721 ggcgtctgcc agtgtaaaag tgggagcaac caccacgctg acctttacag taaaacctga
     9781 cggagccagt gacaaagcga tcagtgtgca ttcgtcagat ccacagactg ccacggttac
     9841 cctgaacgga cttgtggcca cggtaaaagg cgtgaagcag ggcagtgtca gcattgtggg
     9901 catgaccgct gacgggaatt ttgtggctgt gactgcggtg actgtcagcg cagcaggtta
     9961 acaggacgat actcatcatt tgccccggtt attcggggct tttttgcagg tggagaacat
    10021 gatgtttctg aaacaggaca cgtttaatta tgaaaaacag tccgtggtgc tcagtgagct
    10081 gtccgggctg cagagaattg aatatctgac gtttgttcag cagcgaacgg caaagtttga
    10141 tgccggggag ggagaactgc cggaggctga acgacagatt gcttttctgc ggatggggat
    10201 ggatatcaat gcctggctgg tttcccgctc actgtggaat gcggatcagt ctaaggatgt
    10261 agagacgctt tgcgcatccg ttattacaac atggtcgtat gatgccctgg gtgcgggggc
    10321 ggagatggtt ctgtcgctga gcggtatggg ggccattgat aatgccgtgg atgatgagca
    10381 tgaggcgctg acgccggaaa agtcctgacg cgggaaatgc agtttgtcat gcggcttgcc
    10441 cgggagttcc ggcgggcaga ctggcggcgg atgctgtcgg aaatgtcggc cactgagctt
    10501 ggtgagtggg gcgattattt ccggatgcag agcttcagtg atgtgtggat ggatgcgcag
    10561 tttgcctcgc tgaaggcatt gatcgtgaga atggtgtccg gcagcagtga tgctgcggtg
    10621 gctgatttca gccttttacc ggaagagaac gggataccgg agcgaacgga cgaagaactg
    10681 atgcatcttg gggaaggtat ttccggaggt gtgcgttatg gaccagatag ccaacctggt
    10741 cattgatttg gggattgatg cggcagagtt taaaaatgaa attccccgta tcaaaaacct
    10801 tctgaatggt gcagccagcg atgcagaacg gtcttctgcc cgtatgcagc gttttatgga
    10861 gcgtcagact caggccgccc ggcagacaac gcaggcagct tcttcggctg caacagccgc
    10921 atccgtccat gcgcagacgg tggagaagaa cgcacaggct catgaacgca tggcccgcga
    10981 ggtggagaaa acccgccagc gcatggaggc actgagccag aaaatgcgcg aggaacaggc
    11041 gcaggccatg gctctggcgg aggctcagga taaagcggct gctgcgtttt atcgtcagat
    11101 tgacagtgtg aaacaggcca gtgcggggct gcaggaatta cagcgtattc agcagcagat
    11161 ccgacaggcc agaaacagtg gcgggattgg tcagcaggat tatctggcgc tgatttctga
    11221 ggttactgcg aaaacccgtg ttcttacaca ggctgaggaa gaggctaccc gacagaaagt
    11281 ggcgtttatc cgtcagctta aagagcaggc aacccgcctg aatctttcat cttctgagtt
    11341 gcttcgtgct aaggcagccc agctgggggt aagcagtgct gcagaagtgt atatccgcaa
    11401 aatggagcag gcaggaaaag ccacgcattc gctgggtctg aaaagtgcag cagcccgcca
    11461 ggagataggc gttctgatag gtgaactggc ccgcggcaat ttaggggcgc tgaggggatc
    11521 cgggataacg ctggctaacc gtgccggatg gatagacaca ctgatgtcac cgaaaggcat
    11581 gatgcttggc ggggttattg gcggtattgc cgcggccgtc tatggtctgg gtaaagcctg
    11641 gtatgacggt cagaaggagg gggaagaatt taaccgccag ttgtcgctga cggggcatta
    11701 tgccggagtc actgccgggc agctgtggac gctcagtcgt gctatttccg ggaatggtat
    11761 cacgcaacat gctgcagccg gtgcgctggc tcaggtggtg gggagtggtg catttcgtgg
    11821 aaacgatatc ggtatggtgg cgagagttgc cgcacagatg gagcgatcgg ttggccagtc
    11881 ggtcagcgat accataaatc agtttaagcg gctgaaggat gatcctgtaa atgccgcgaa
    11941 ggctctggac aatgagctgc attttcttac tgccactcag cttgagcaga tacgcgtcct
    12001 tggggagcag gggcggtcca gtgatgctgc acggatagcc atgtctgcac tggcagagga
    12061 aaccggtcgg cgtactgcgg atattgataa taacctcaat gcgcttggca gtacgctgaa
    12121 gtatctgtct gatttgtgga gtcgtttctg ggatgcggcc atgaatattg gtcgtgaaga
    12181 ctcgctggat gaacagattg ccgctttaca ggagaaagtg tcgcgggcga aaagactccc
    12241 ctggacggca tcatcttctc aggttgaata cgatcagcag cgtcttaacg atcttcagga
    12301 gaaaaaacgc cagaaggatt tgcaggatgc aaaagagcag gcagagcgga attatcagga
    12361 gcaacagaaa cgccgtaatg ctgaaaatgc tgcactgaac cggatgaatg aaacggaagc
    12421 agcacgacat cagcgtgaaa ttgcgcgtat taatgccatg cagtacgccg atcaggctgt
    12481 cagggatgcg gcgatacaac gtgaaaatga acgttacgag aaagccctgg catccggtaa
    12541 gaaaaaaaca cgcgaaaccc gtaatgatga ggccacccgg ttattgctgc agtacagtca
    12601 gcaacaggca caggtggaag gacagattgc tgctgccaga cagtcagcag gcattgccac
    12661 ggaaaggatg acagaagcgc ataaacagct tctggctctg cagcagcgca tcagcgacct
    12721 ggacgggaaa aaactgacgg cagatgaaaa gagtgtgctg gcccgtaaag atgaactgat
    12781 tcaggcactg acgctgctgg atgtaaaaca gcaggagctt cagaaacaga cggcactcaa
    12841 cgagctgaag aaaaaaacaa ttcagctgac cagtcaactg gctgaagaag agcgcgctca
    12901 gcgtcagcaa catgacctgg atatcgccac ggtgggtatg ggtgatcagc agcgacagcg
    12961 atatcaggta caactgagtc ttcgccagaa ataccagcaa cagctggagc agttgaggcg
    13021 ggatagtgag cagaaaggga catataacac ggatgactac agaaaggccg agcaggcgct
    13081 gacggagagc ctgaaccgac aactgaatga gaatcgccgt tactggcaac agcttgaagt
    13141 tgtgcagggt aactggaaaa acggagtcct gcgtgcattt caggatttta ccgtggatgc
    13201 agataatacg gcagaaacag cagaacaggt gttctcgtca gccttcagca acatgggaaa
    13261 tggcctggca acttttgtca ctaccggcaa actcaatttc aaatccttca cctcttctgt
    13321 gctgtcagat atggcgaaaa tcctggcgca ggcaaccatg atgaaatcga taaaagggat
    13381 tggcagtgta ctgggatttg atctcagcag cctttccctg aatgccaatg gggggattta
    13441 tcagtctgct gatttgagcc gttacagtgg cacggtggtt aaccgtccga cgttttttgc
    13501 ttttgcaaaa ggcgcgggtg tgatggggga agcgggacct gaagccattc tgccattgcg
    13561 tcgtggtgct gacggtaagc tgggggttgt ggcggatatt ggggggtcag gtatggcgat
    13621 gttttccccg cagtacaaca tcgagatcaa taacgatggc acgaacgggc agataggtcc
    13681 ggctgccctg aaggcggttt atgacctcgg gaaaaaagcg gcagcggact ttatgcaaca
    13741 gcaggcccgt gatggtggtc ggttaagtgg agcatatcgg taatggagac gtttcactgg
    13801 aaagtgcgcc cggatatgaa tgtggtatca gagccgaaag tggtgacagt gaagctgggc
    13861 gatggttatg aacagcgtcg tgcggcggga ctgaataacc agttgtcgac ttacagcgtg
    13921 acgatacgtg ttcgtaaatg tgaccaccca tctttaaaag cctttctgga acggcacggt
    13981 ggcgtccgtg catttcagtg gacgccacct tatgactgga aaccgatcag ggtggtttgt
    14041 cgtaaatggt cggcaagcgt gggggcgctg tgggtaacca taacggcaga ttttgaacag
    14101 gtcgtggcat aggaggctct gatgcaggat attccacagg aaacacatca tgagacgaca
    14161 cgcctcactc agtcagccca ggtggtgctc tgggaaatcg atctgacaga ggtcggtggt
    14221 gaacgttatt ttttctgtaa tgagcagaac gaaaaaggtg agccggttat ctggcagggg
    14281 cggcagtatc aggtataccc cattcagggg acgggatttg aactgaacgg caagggcagt
    14341 gctgcccgtc cgacactgac ggtttctaac ctgtacggta tggtcaccgg gatggcggaa
    14401 gatctgcaga gtctggtcgg cggaacggtg gtccggcgta aggtttacgc ccgttttctg
    14461 gatgcggtga acttcgtcaa cggaaacagt gacgccgatc cggagcagga ggtgatcagc
    14521 cgctggcgca ttgagcagtg cagcgaactg agcgcggtga gtgcctcttt tgtactgtcc
    14581 acgccgacgg aaacggacgg cgctgttttt ccgggacgta tcatgctggc caacacctgc
    14641 acctggacct atcgcggtga tgagtgcggt tatcacggtc cggcagtcgc ggatgaatat
    14701 gaccagccga cgtccgatat cacgaaggat aaatgcagca aatgcctgag cggctgtaag
    14761 tttcgcaata acgtcggcaa ctttggcggc ttcctttcca ttaacaaact ttcgcagtaa
    14821 tcccatgaca gagacagaat cagcgattct ggcgcacgcc cggcgatgtg cgccagcgga
    14881 gtcgtgcggc ttcgtggtga gagcgccgga gggggaaaga tattttccct gcgtgaatat
    14941 ttccggtgag ccggaggatt atttccggat ggctccggag gactggctgc aggcagagat
    15001 gcagggtgag attgtggcac tggtccacag tcatcccggt ggtctgccct ggctgagtga
    15061 ggctgaccgg cggctgcagg tgcagagtga tttgccgtgg tggctggtct gccggggggc
    15121 gattcacaag ttccgctgtg tgccgcatct caccgggcgg cgctttgagc acggggtgac
    15181 ggactgttac acgctgttcc gggatgctta tcatctggcg gggattgaga tgccggattt
    15241 tcatcgcggg gatgactggt ggcgtaacgg tcagaatctc tatctggata atctggaggc
    15301 cacagggctg tatcaggtgg cgttgtcatc agcacaaccg ggcgatgtgc tgctgtgctg
    15361 ttttggttca tcggtgccga atcatgccgc catttactgc ggcgacggtg agctgctgca
    15421 ccatattcct gaacaactga gtaaacgaga gaggtatacc gacaaatggc agcgacgcac
    15481 acactccctc tggcgtcacc gggcatggca cgcatctgcc tttacgggga tttacaacga
    15541 tttggccgcc gcatcgacct tcgagtgaaa acggggtctg aagccatccg ggcgctggcc
    15601 atgcagatcc cggcgtttcg tcagaaactg agcgacggct ggtaccaggt acgcattgcc
    15661 gggcgtgatg caggtgaaac cgaattgtct gcccgtctta atgagccgct ggcaaatggt
    15721 gccgtgatcc acatcgtgcc gcgtctggcg ggagctaaaa gtggcggtgt gtttcaggtg
    15781 gtgctggggg cggcgctgat tgcggtggca tggtggaacc ctgtgggctg gctgggtgcc
    15841 gcggctgtat cgggcatgta tgcggcaggg gccagtatga tcctgggcgg agtggcgcag
    15901 atgctggcac cgaaagccag gacgcccacg gcagcaagta cagataacgg caaacagaac
    15961 acctatttct cctcactgga taacatggtt gcccagggca atgttctgcc cgttctgtac
    16021 ggtgaaatgc gcgtggggtc gcgggtggtc tctcaggaga tcagcacggc agacgaaggg
    16081 gatggtggtc aggttgtagt gattggtcgc tgatgaaaaa cgttttatgt gaaaccgcct
    16141 ccgggcggtt ttgtcgttta tggagcgtta ggaatgggta aaggcagcag taaggggcat
    16201 actccgcgcg aagcgaagga caacctgaag tccacgcagt tgctgagtgt gatcgatgcc
    16261 atcagcgaag ggccgattga aggtccggtg gatggattaa aaagcgtgct gctgaacagt
    16321 acgccagtgc tggacactga ggggaatacc aacatctccg gcgtcacggt ggtgttccgg
    16381 gccggtgagc aggagcagac accgccggag ggatttgaat cctccggatc cgagacggtg
    16441 ctggggacgg aagtgaaata cgacacgccg atcacccgga ccatcacgtc ggcaaacatc
    16501 gaccgtctgc gctttacctt cggtgtgcag gcactggtgg aaaccacctc aaagggggac
    16561 aggaatccat cggaagtccg cctgctggtt cagatacaac gtaacggtgg ctgggtgacg
    16621 gaaaaagaca tcaccattaa aggcaaaacc acctcgcagt atctggcctc tgtggtggtg
    16681 gataacctgc cgccgcgccc gttcagtatc cggatgcgca ggatgacgcc ggacagcacc
    16741 acagaccagc tgcagaacaa aacgctctgg tcgtcataca ccgaaatcat cgatgtgaaa
    16801 cagtgctacc cgaacacggc actggtcggc gtacaggtgg attcggagca gttcggcagc
    16861 cagcaggtga gccgtaatta tcatctgcgc gggcgtattc tgcaggtgcc gtcgaattat
    16921 aacccgcaga cgcgacaata cagcggtatc tgggacggaa cgttaaaacc ggcatacagc
    16981 aacaacatgg cctggtgtct gtgggatatg cttacccatc cgcgctacgg catggggaaa
    17041 cgtcttggtg cggcggatgt ggataaatgg gcgctgtatg tcatcggcca gaattgcgac
    17101 cagtcagtgc cggacggctt tggcggcacg gagccgcgca tcacctgtaa tgcgtacctg
    17161 accacacagc gcaaggcgtg ggacgttctc agtgatttct gctcggcgat gcgctgtatg
    17221 ccggtatgga acgggcagac gctgacgttc gtgcagaacc gaccgtcgga taaggcgtgg
    17281 acctataacc gcagtaatgt ggtgatgccg gatgatggcg cgccgttccg ctacagcttc
    17341 agcgccctga aagaccgcca taatgccgtt gaggtgaact ggattgaccc gaacaacggc
    17401 tgggagacgg cgacagagct tgttgaagat acgcaggcca ttgcccgtta cggtcgtaat
    17461 gttacgaaga tggatgcctt tggctgtacc agccgggggc aggcacaccg cgccgggctg
    17521 tggctgatta aaacggagct gctggagacg cagaccgtgg atttcagcgt cggcgcagaa
    17581 gggcttcgcc atgtaccggg cgatgttatt gaaatctgcg atgatgacta tgccggtatc
    17641 agcaccggtg gtcgtgtgct ggcggtgaac agccagaccc ggacgctgac gctcgaccgt
    17701 gaaatcacgc tgccatcctc cggtaccacg ctgataagcc tggttgacgg aagtggcaat
    17761 ccggtcagcg tggaggttca gtccgtcacc gacggcgtga aggtaaaagt gagccgtgtt
    17821 cctgagggtg ttgctgaata cagcgtatgg gggctgaagc tgccgacgct gcg
//
LOCUS       DAZFWL020000092        17846 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFWL020000092 DAZFWL020000000
VERSION     DAZFWL020000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.092"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..619)
                     /locus_tag="ACHKNP_RS16635"
                     /old_locus_tag="pgaptmp_003327"
     CDS             complement(<1..619)
                     /locus_tag="ACHKNP_RS16635"
                     /old_locus_tag="pgaptmp_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705443.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKTLVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHTESSALPEVRLRASELIRLYPKEIIFFICVGVLVG"
     gene            complement(635..1039)
                     /locus_tag="ACHKNP_RS16640"
                     /old_locus_tag="pgaptmp_003328"
     CDS             complement(635..1039)
                     /locus_tag="ACHKNP_RS16640"
                     /old_locus_tag="pgaptmp_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4705444.1"
                     /translation="MKNDIGQRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(1129..1266)
                     /locus_tag="ACHKNP_RS16645"
                     /old_locus_tag="pgaptmp_003329"
     CDS             complement(1129..1266)
                     /locus_tag="ACHKNP_RS16645"
                     /old_locus_tag="pgaptmp_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705445.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            1338..1541
                     /locus_tag="ACHKNP_RS16650"
                     /old_locus_tag="pgaptmp_003330"
     CDS             1338..1541
                     /locus_tag="ACHKNP_RS16650"
                     /old_locus_tag="pgaptmp_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4705446.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            1563..1913
                     /locus_tag="ACHKNP_RS16655"
                     /old_locus_tag="pgaptmp_003331"
     CDS             1563..1913
                     /locus_tag="ACHKNP_RS16655"
                     /old_locus_tag="pgaptmp_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HGU4705447.1"
                     /translation="MKKNTNNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHTWLIEKAGIVVHRCPHNPFTGNRIFALSSGDNQFGQDFTLYEALRTVD
                     RLLRGQSFIKQAAL"
     gene            1924..2202
                     /locus_tag="ACHKNP_RS16660"
                     /old_locus_tag="pgaptmp_003332"
     CDS             1924..2202
                     /locus_tag="ACHKNP_RS16660"
                     /old_locus_tag="pgaptmp_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705448.1"
                     /translation="MTKDHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQIIREIISEAEKSRQVNGDEPYIEIICMDSEAEIQKAD"
     gene            2214..2456
                     /locus_tag="ACHKNP_RS16665"
                     /old_locus_tag="pgaptmp_003333"
     CDS             2214..2456
                     /locus_tag="ACHKNP_RS16665"
                     /old_locus_tag="pgaptmp_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HGU4705449.1"
                     /translation="MDKEYKTLINKALERFYFRLSASGAHSERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            2453..2566
                     /locus_tag="ACHKNP_RS16670"
                     /old_locus_tag="pgaptmp_003334"
     CDS             2453..2566
                     /locus_tag="ACHKNP_RS16670"
                     /old_locus_tag="pgaptmp_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705450.1"
                     /translation="MSIFISWIVLIISVVCAIGIMRIINSVKKIERFFTGE"
     gene            2653..2856
                     /locus_tag="ACHKNP_RS16675"
                     /old_locus_tag="pgaptmp_003335"
     CDS             2653..2856
                     /locus_tag="ACHKNP_RS16675"
                     /old_locus_tag="pgaptmp_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705451.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQDPRK"
     gene            3180..3569
                     /locus_tag="ACHKNP_RS16680"
                     /old_locus_tag="pgaptmp_003336"
     CDS             3180..3569
                     /locus_tag="ACHKNP_RS16680"
                     /old_locus_tag="pgaptmp_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HGU4705452.1"
                     /translation="MIDAHDFTRWVRTQDTRLAPILRGLFDLYIRGRDNRARTTKPEN
                     VDTLYFTVDDCYRVDFTPHGLALHCLTPHGESLLAYYDSPASVFAAMLAHRTAGGCVS
                     LSEYTAEFNRLSTIFLQEWQRVTGYQP"
     gene            3566..6406
                     /locus_tag="ACHKNP_RS16685"
                     /old_locus_tag="pgaptmp_003337"
     CDS             3566..6406
                     /locus_tag="ACHKNP_RS16685"
                     /old_locus_tag="pgaptmp_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HGU4705453.1"
                     /translation="MSAFAWSWNEPRPAIDPARFTERRQETETDLQRAIRYYLEANKR
                     AQEEQEAKEEAFFAQSTVGKKLMASLEEAGQREKLAQSIISKRQATEQDPVARAFATL
                     KVLPVYLREPLSRHLSFLRKKQEADRQKGKKSWQAERYARGTLRKIFERLDRTDSRWL
                     TPGYRSLAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSGTFEKLCDGFGATDGELTM
                     DVTLKAYQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRK
                     LWLLRCEWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFT
                     IDLETVYYAGVSNPIHRKAEMMATMKGLELLAEARGDRAVFLTVTCPSKYHATTENGH
                     PNPKWNGATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFA
                     HPEEIDTIVSHTRDIAIQEDRHELGNDITPRFKVEYVDGSKGTPTSYIATYIGKNLDS
                     RAVDGIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRR
                     LASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLA
                     DELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSTRENGFDLQGGP
                     AAPWTRGNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRS
                     LRDKQAKNSSDEMQNWTQSRKQPRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLR
                     GGSLYVDGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLE
                     QLGASDPEAAAHLASTLQQDQNTMKTPVTVLSDMLRAIRDAVHAQRISETTERAHRKA
                     DLLRGSLTSGNKKQTETGLTNPVNEQKTCRDI"
     gene            6483..7442
                     /locus_tag="ACHKNP_RS16690"
                     /old_locus_tag="pgaptmp_003338"
     CDS             6483..7442
                     /locus_tag="ACHKNP_RS16690"
                     /old_locus_tag="pgaptmp_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HGU4705454.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNIIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            7447..7758
                     /locus_tag="ACHKNP_RS16695"
                     /old_locus_tag="pgaptmp_003339"
     CDS             7447..7758
                     /locus_tag="ACHKNP_RS16695"
                     /old_locus_tag="pgaptmp_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGU4705455.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            7822..8412
                     /locus_tag="ACHKNP_RS16700"
                     /old_locus_tag="pgaptmp_003340"
     CDS             7822..8412
                     /locus_tag="ACHKNP_RS16700"
                     /old_locus_tag="pgaptmp_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4705456.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADEHYEKSRVTVLDVGDPEVLALLDWLETKDNRVAELEKIATDYA
                     LKFQKAQDALKYAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNRGWFSPLTTDKNNT"
     gene            8706..8834
                     /locus_tag="ACHKNP_RS16705"
                     /old_locus_tag="pgaptmp_003341"
     CDS             8706..8834
                     /locus_tag="ACHKNP_RS16705"
                     /old_locus_tag="pgaptmp_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705457.1"
                     /translation="MKGKAVKIALRRREHAVNAVGLRKSLTVDGGDKLEGVVKLSD"
     gene            complement(8903..9949)
                     /locus_tag="ACHKNP_RS16710"
                     /old_locus_tag="pgaptmp_003342"
     CDS             complement(8903..9949)
                     /locus_tag="ACHKNP_RS16710"
                     /old_locus_tag="pgaptmp_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4705458.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(9949..11700)
                     /locus_tag="ACHKNP_RS16715"
                     /old_locus_tag="pgaptmp_003343"
     CDS             complement(9949..11700)
                     /locus_tag="ACHKNP_RS16715"
                     /old_locus_tag="pgaptmp_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HGU4705459.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATVTGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKATHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            11855..12691
                     /locus_tag="ACHKNP_RS16720"
                     /old_locus_tag="pgaptmp_003344"
     CDS             11855..12691
                     /locus_tag="ACHKNP_RS16720"
                     /old_locus_tag="pgaptmp_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HGU4705460.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            12715..13767
                     /locus_tag="ACHKNP_RS16725"
                     /old_locus_tag="pgaptmp_003345"
     CDS             12715..13767
                     /locus_tag="ACHKNP_RS16725"
                     /old_locus_tag="pgaptmp_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HGU4705461.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNSSYDGIKRRNPNNVIDMK
                     SRQYQCEQVNYDTFISYPQLDTWAAHSDFQSRISTQIARQVALDRIMIGFNGTSHAYE
                     SDFHTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            13813..14613
                     /gene="gpM"
                     /locus_tag="ACHKNP_RS16730"
                     /old_locus_tag="pgaptmp_003346"
     CDS             13813..14613
                     /gene="gpM"
                     /locus_tag="ACHKNP_RS16730"
                     /old_locus_tag="pgaptmp_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HGU4705462.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAACTAHEEVLYRLRLAQA
                     RLKAIQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLWEI
                     VANQDIPDEVRAKLCKACAFARRGLTDADNMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKNKKTTRKPATRKTTATQKAKRG"
     gene            14715..15209
                     /locus_tag="ACHKNP_RS16735"
                     /old_locus_tag="pgaptmp_003347"
     CDS             14715..15209
                     /locus_tag="ACHKNP_RS16735"
                     /old_locus_tag="pgaptmp_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HGU4705463.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAIAHVTRELEAWQQEQQAAGYQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILGRPRMF
                     AELC"
     gene            15209..15409
                     /locus_tag="ACHKNP_RS16740"
                     /old_locus_tag="pgaptmp_003348"
     CDS             15209..15409
                     /locus_tag="ACHKNP_RS16740"
                     /old_locus_tag="pgaptmp_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HGU4705464.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTRGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            15412..15735
                     /locus_tag="ACHKNP_RS16745"
                     /old_locus_tag="pgaptmp_003349"
     CDS             15412..15735
                     /locus_tag="ACHKNP_RS16745"
                     /old_locus_tag="pgaptmp_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU4705465.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGIPEDAVTFFGASIGFMGAEKARDKVIAIFDRRV
                     KERNE"
     gene            15732..16124
                     /locus_tag="ACHKNP_RS16750"
                     /old_locus_tag="pgaptmp_003350"
     CDS             15732..16124
                     /locus_tag="ACHKNP_RS16750"
                     /old_locus_tag="pgaptmp_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HGU4705466.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVTTGKSQTMNSRHLTGHAVDVVAYVGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            16121..16528
                     /locus_tag="ACHKNP_RS16755"
                     /old_locus_tag="pgaptmp_003351"
     CDS             16121..16528
                     /locus_tag="ACHKNP_RS16755"
                     /old_locus_tag="pgaptmp_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HGU4705467.1"
                     /translation="MKLWPTLGVAFLLIAAWGTSMRLSWSLGRENARNEAQASTLKST
                     VDTLNIISAGVQDMQQVLAQLRADNQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHAGNASPLP"
     gene            16667..>17846
                     /locus_tag="ACHKNP_RS16760"
                     /old_locus_tag="pgaptmp_003352"
     CDS             16667..>17846
                     /locus_tag="ACHKNP_RS16760"
                     /old_locus_tag="pgaptmp_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4705468.1"
                     /translation="MTSKNFALITAMTQAELTQKVNEHLAKGWHLQGETRVAYEPGTP
                     WYLMQAMVADGTTDISPDSPQHGSVPEWYYVVVLAGQSNAMSYGEGMPLPDSYDAPHP
                     RIKQLARRNTVTPGGKACAFNDIIPADHCLHDVQDMSALNHPNADLSKGQYGCVGQGL
                     HIAKRLLPYIPQNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQD
                     LILRTKAALQKNPKNMLLAVCWMQGEFDMSAATYSQQPPLFAAMLKQFRADITEFNTQ
                     CHGGRAASVPWICGDTTYYWKNTYGTQYDTIYGAYKNRESDNVFFVPFLTDGSGNNTS
                     TNAPTEDPDAASEGYYGSASRTNKNWVSSNRQTHFSSWARRSIIPDRMATAILNVAGR
                     T"
BASE COUNT         4598 a         4817 c         4636 g         3795 t
ORIGIN      
        1 cgcctaccaa aacccctacg cagataaaga atattatttc ttttggataa agtcggatta
       61 attctgaagc acgaagtcga acttctggta aggccgaact ctctgtgtga cttgatgccg
      121 attgttgctc taaccacgac aatgcagact gtaactgaga acgagtaaga tcgtttaaac
      181 gtcctgtacc gaaattgata tggcaatacc gcagaagttt ttgtcgaagt ccacagtctt
      241 cactgttacg tagtaataaa cttacaagag tcttacaggc atcatgatct ttacatcgct
      301 caagcattgc atgcagaaaa ctctccgctg ttttatattg atttactgtc atatcatcaa
      361 taccagctac accaatctcc gcatgtactt tttgccaaat aataaacgct tcagtattgc
      421 tagcttctgc aatagcagca accaagctat ttagctcctt acgctgagcc ttaagcaaag
      481 ggcgatcgtc atcatcatta ttcgaaggga ttgcgatatt gacggtatga gaaccatcat
      541 atcgctctat ctgaatattc ttttcgtgaa aatcacgccc agcaacgcga ttgtttgaac
      601 cgtttgagtt gacggccatg tcacctccct actatcacct acccttagtt tcgttatagt
      661 cacgaccagc gatacggtta ttaccaccag aaatatttaa ctcacgtcct gatggctgag
      721 tttccttttc actgatcgca ccttttaaag ccccgatcac cgcgtttttc acatctaacg
      781 aagctgctcg aaagcgagta atcaactcct gctcatcatc gttataagtt tcaggtgagt
      841 gaattcccaa cacaacatac tgaacatcaa ggccaaaacg agacagcgct gccaaatacg
      901 cagcatcagg aaagctatct cctttctcat atctaagctg agttagcttt ttgactccac
      961 caatgtcgct catggcaact tgactaagtc ccaatctttc cctttcctca cgcaaccgct
     1021 gaccaatatc atttttcata caaaaacctt gacaggtatc ttttttgata ccaaaatgat
     1081 ttcacgagct attagatgat cacaatatac cactatgaaa caagttcttc acgataccag
     1141 atcacgcatt ccgcgtaaca ccgccacagg tccaagactg gcacttcggc tgtccctcga
     1201 ggagcgagcc gtcattgatg aaatggcagc taaagaacaa cgctcatcct ctaacatggc
     1261 gcgcatgatc ttccttcgcg gcctagagct aacccagaaa gaacaaaaca aatcttcctg
     1321 atcaggaggc tagtgggatg tcaggtataa ccatcaatat caatgtgaat gccccctatg
     1381 tatccctgca gaaatatgca gagataacag gtatccctct taatacatgc aaaaagatgt
     1441 tggctgacgg tcgaattatt atccgcccca aacgcgccaa aatggaaaag cctgaagtaa
     1501 accttgtggc gatgttaaaa gacgctttgg ctaacagcta aaacaatgaa cagagcatca
     1561 tcatgaaaaa aaacactaat aatccatact ccaaatttcg taatggcgta gaacgccatg
     1621 tacaccacgt cgctaccagt gcatcacgta gtaacagccg ctataacctg aacgagacgc
     1681 acgcaacacc agatgggcac gctgtaaaac aaatcggcga acacacctgg ctgattgaga
     1741 aagctggaat cgtggttcac agatgcccac acaatccgtt taccggaaac cgcatttttg
     1801 cattaagcag cggcgataat cagttcgggc aggatttcac attgtacgaa gcactacgca
     1861 cagtcgatcg tctgcttcgc gggcaaagtt ttattaaaca ggctgcttta taacaggtgc
     1921 tttatgacta aagaccatgc acaaggtgta tttatccgct ttattgattt tcgcggtgaa
     1981 ctgttattac gcgcatcagc tattgacgga gtagttccat ctgaaaaaaa cgcagctacc
     2041 tacgtttatc tgaacggtac acgcctgact gtagagcttc cgtaccagat cattcgcgaa
     2101 ataattagcg aagccgaaaa atctcgtcag gttaatggcg atgaacccta tatcgagatt
     2161 atctgcatgg attcagaagc tgaaattcag aaagcagatt aaagggtgtt gcgatggata
     2221 aagaatataa aactctcatc aacaaagcac ttgagcgttt ttattttcgt ttaagcgcat
     2281 ccggcgctca ttcagaacgt gcagcccgtg actcattgac cagggctatc cggagtctgt
     2341 atgacgtagc tttttacgct gacgatctgg atgcacttaa tgaactttct gagcttatct
     2401 gcgccgcaga atgcggggag catattgagc cgtacaaact gggaaatatc gcatgagtat
     2461 atttatatcc tggattgttc ttattatttc ggtggtttgt gcaattggta ttatgcgaat
     2521 tattaattca gtaaaaaaga ttgaacgctt tttcactggc gaataaccgc ataaataaaa
     2581 ccccagctta aataaaaaaa tgtgaacaca atctgcattc gcagaggtat tcacacacac
     2641 caaggaggcg taatggcaat taagcatttt cctgtcgttc gtttcacctc cagaggacgt
     2701 gaatacgaag tcgacgaacg tctgattacc acaatcgaca aacaccgctc agaaaaggat
     2761 gcacaccaca tctatctcac tgacggcact tacttttgcg ccactaatgt ggcgcgggtg
     2821 aatcttatcc gacaggtaca ggatccacgc aaatgagtag gagaagaatc actcgcagac
     2881 atcaccgaac acacctgagt tcctcagcaa cgctaaaggc acttattcaa agcgagatcg
     2941 gtgatttctt cgcgggagtt ggctcaccag gcgaaccaga aacaccagaa gcgatgcagc
     3001 gtgagctcat gatacgcata gataacactt ttgatttctt ctacagcatg cacggaatta
     3061 aacagaaatg aacctcaagc cagcaataac tactcgtaga acgtcaattc ctgtaaccga
     3121 ccgcttctga gttttttggc aggaagcctt cgcacatctg tagtaaagag aattgcagca
     3181 tgattgacgc tcatgacttc acaagatggg tgcgcacaca ggacacccgt ctggctccca
     3241 ttcttcgggg attatttgat ctctacattc gtggtcgtga caacagagca cgcaccacaa
     3301 aaccggagaa tgtggatacc ctttatttca cagtagacga ctgctaccgc gtggacttca
     3361 caccacacgg actggcgttg cactgcctga caccgcacgg cgaatcactg ctggcgtatt
     3421 acgactcccc ggcctccgta tttgcggcaa tgctggcgca tcgcactgct ggcgggtgtg
     3481 tctcgctgag tgaatacacc gctgaattta accgcctttc caccatcttc ttgcaggagt
     3541 ggcagcgcgt gacgggatac cagccatgag tgcgtttgca tggagctgga atgaaccacg
     3601 gccagccatt gatccggcca gatttacgga gcgcaggcag gaaactgaaa ccgacctgca
     3661 acgcgccatc cgttactacc ttgaggcaaa caaaagggca caggaagaac aggaagcgaa
     3721 ggaggaagcc tttttcgcac aatccaccgt gggtaaaaaa ctcatggcat cccttgagga
     3781 agccggacag cgtgaaaagc tggcacaaag catcatcagc aagcgtcagg caacagaaca
     3841 agacccggtg gcccgtgctt ttgccacact gaaggtgctt cccgtttatc tgcgtgaacc
     3901 tctgagccgc cacctctctt ttctgcgcaa gaagcaggaa gccgatcgtc agaaaggcaa
     3961 aaagagctgg caggctgaac gctacgcgcg cggaaccctg cgcaaaatat tcgaacgtct
     4021 ggaccgcacc gacagccgct ggctgacacc gggctatcgc tcccttgccg gacgcgaacg
     4081 cctggacgat ttgctttacc tgccgcagct caacaaacac cagatacaga cgctggccac
     4141 catgacggcg gcgatgttca gtggcacttt tgaaaaactc tgcgatggct ttggcgcgac
     4201 cgatggcgag ctgaccatgg atgtaacgct gaaggcgtat cagatgctgg cccgcatggc
     4261 gttacacctg cacgctatgc ctccacatta tgacgctctg acaacagaca aagaccggag
     4321 gaatgaaccg gacacggagc tgctgccggg cgcaatcctt cgcctgacct gtgcggaatg
     4381 gtggaaacgc aaactgtggc tgttacgttg cgagtggaga gaagaacaac tccgcgccgc
     4441 ctgtctggtt tccagaaaaa catcacccta tctgagccag gacgcgttaa gcgagtttcg
     4501 cgcacagcgc gagaaaacac gcgatttcct gaaaagtttc atgctggaaa acgaagacgg
     4561 gttcacgatt gatctcgaga cagtgtatta cgcgggagta agtaacccga ttcaccgtaa
     4621 ggcagaaatg atggccacca tgaaggggct ggaacttctg gccgaagccc gtggcgacag
     4681 agcggtgttt ctgactgtca cctgcccgtc aaaataccac gccacaacag agaacggtca
     4741 tccgaatccc aaatggaacg gggccaccat gcgcgactcc agcgattacc tggttaacac
     4801 gttttttgcg gcggtccgca agaaactgaa ccgcgacggc ctgcgctggt atggcatccg
     4861 cacggtggag cctcaccatg acggcaccgt gcactggcat atgatggtct ttgctcatcc
     4921 ggaagaaatc gacaccattg tgtcccacac ccgcgatatt gccattcagg aagaccgcca
     4981 cgagctgggc aatgatatta ctccgcgctt taaggtggag tatgtcgacg gctcaaaagg
     5041 cacgccaacc agctacatcg ccacctacat cggaaagaac ctggacagcc gcgccgtgga
     5101 tggcatcgac ccgaaaacgg gcaagccacg cgttgaccac gaaaccggaa aatcaatggc
     5161 cgagagcgtg gaacgcgcca tcggctgggc acgccttcac cgggtccgcc agttccagtt
     5221 ctttggcatc ccctcccgtc aggtatggcg tgaactccgc cgccttgcca gccagatggc
     5281 acgcaacccg gaaggcccac aacggctgaa ggatgatgca atggatgcgg tactcgctgc
     5341 cgctgatgcc gggtgttttg ccacctacat tgagaaacag ggcggcgtac ttgttccacg
     5401 caaagactac ctgattcgca ccgcctacga cctcgcagat gagctgaacg attacggcga
     5461 acagagcgta cagatttacg ggatctggtc accactcatc ggggaatcct cccgtgtgtg
     5521 cacgcacccg gataactgga agctggtaag acgtaaaccg gaagcggaag acagcacccg
     5581 cgaaaatggt tttgaccttc agggcggccc tgccgcccct tggactcgtg gcaataactg
     5641 tccccgtgta caggaaacgg acaacaacgg gacagaacag ccggaagaaa ggccagcacc
     5701 gtggccgcag cttcctgacg gcgttgacgt gaatgaatgg atgcgctcac tgaaacggca
     5761 cgaacgccgg gcgctgatgc gttcgctgcg tgacaaacag gcaaaaaaca gcagtgatga
     5821 aatgcagaac tggacacaga gccgcaaaca gccacggcct ttgcctgata accacgagtt
     5881 actcgctaaa gaatggcggg agtctgctga atctctcggc ctgcatatcg gtgaacagca
     5941 gatgcagcac ctgttacggg gcggcagtct gtacgttgac ggcagcatca ttgcaccgca
     6001 gggatttgaa attgtacgca aaccagatac ccgcccggac agccgaatca cgcaactctg
     6061 gcagcgcctg agccgtaatc acggcgtaag cagcacggag atccgccata acccggtcgc
     6121 cagctatctg gaacagctgg gggcatcaga ccccgaagcc gccgcacacc tggcatccac
     6181 acttcagcag gaccagaaca ccatgaaaac ccccgttacc gtgctttctg acatgctgcg
     6241 cgccatccgt gacgcagtgc acgcacagag aatcagtgaa accactgaac gcgcccaccg
     6301 caaagcagac ctgctgcggg gtagcctgac cagtggaaac aaaaaacaga cagaaacggg
     6361 actcacaaat cccgtaaatg agcaaaaaac gtgccgcgat atatgaagcg cgcacaaaac
     6421 aggcaaaagc gggatttaaa aatcctgtaa ccgattaatt aatcaacaca aggaaaagcg
     6481 acatgaaaat ttgtatcgac gacggctcca ccaacatcaa gctggcgtgg actgagaacg
     6541 gcgaacgccg caacgccatc agcccgaaca gcttcaagtc ggaatggtct gcgccgttcg
     6601 gtggcacgca gcccgcgaac tacatgcttg atggcgtgcg ctatggtttt gatccggtca
     6661 gcgatcgctt tgtccagacg accgacacgc aataccagta cagcgatgtg aatataattg
     6721 ccattcatca cgcgctggtc aaatcaggca tcacaccaca ggaagtggat gtggttgtca
     6781 ccctgccact gagcgagtat ttcgatacaa acgcacagcc ggacatggcc aacatcaacc
     6841 gcaaaaaagc gaacgtcatg cgcccggtgg agtaccagaa cggcgaagca ttcactatcc
     6901 gtaacgtacg ggttatgcct gaatccattc cggctggctt taaggcactg gctgacatga
     6961 gtccgtttga atccctgctg attgtggatt tgggcggaac cactctggat gtggcaaagg
     7021 ttcaggggca actggcaggt atcagccagg tgttttgcga tccacacgtg ggcgtttctc
     7081 tgatggccga tgccgtactg tcggtgatgg ccactaacgg tatgcgcacc agtcaccaca
     7141 tcgccaatac cattatcgaa catcgccatg atgaagcctg gctgcgccag cacatccaca
     7201 atgacgcgca ttacgccagc ctgatggcgg ttattcgtga aaaggaagaa acactgaaac
     7261 aacgcgtgat ccgcgcgctg gcgggttttt cgggttacgg gcgggtgatg gttgtcggtg
     7321 gaggggcgga gattgtggca cccgctatcc gcgaagcctg cggagttaat gcgactttca
     7381 tcgcggacgg ggtgccacag tttgctctgg ttaatgggct gtacgcaatg gacaaggagt
     7441 aaaccaatga cgactccaac cagacggata agtttctatc tgaagcccac cgccgtcaag
     7501 aacgaaggag aagcatgcgc ctggctggac agccttacac cagaagcccg caaaagcggc
     7561 caacgcgtgg cttttctggc cgggctggca cttctgaaaa tgaatccggc agaggcttat
     7621 cgactggctg catgggctgg tgatgaagcg ttatcagtga cacaaaccag gacagaacgc
     7681 cccgcatcac agccagtatc aaccgcacag ataaccagtc agatggccgg aaatatccgg
     7741 gcgttatttc ctgaataaca caacatcagg gcgagttcgc cctgctctcc acctcagaac
     7801 ataaacaagg agaacgactt aatgagcgaa atcaactatc aggcattacg tgaacgttat
     7861 tcacctgtgc cagtaccgaa atgccctatt tgcggcgagg aaatgtcaat tcagcgaata
     7921 tctggagcac aggttgttta tgcctgctcc ggttatggtg atgatggaga tttcaaaatt
     7981 ggccgaactc ttgccgacga acattatgaa aaatcacgcg taacagtgtt ggatgtcggg
     8041 gatcctgaag tattggcgct acttgattgg ctggaaacca aagacaaccg agtcgctgaa
     8101 ctggaaaaaa tcgccactga ctatgcactt aaattccaga aagcacagga cgcattaaag
     8161 tatgccgctt tgctgcatag caggacggcg caacaaacaa ataattttgc agtatcgctt
     8221 ccggacataa gcgaatattt cattaatgac gtatttcagc ccttgcgata cgagcgggat
     8281 gttgaaagag ccatcataaa ggctggcgga aaagcattgt ggcaggagaa acacgaggac
     8341 agaacgcatc agtcctgcga tgtaaatcgt ggatggttta gcccactgac gacagataaa
     8401 aataacacct gatccccctc aaaccagggc gataatcgcg catcgccctg ctgcacaata
     8461 gtgcacaagt ttgcacaatt tttttgaacg actttttacc cttccggccc gcatggcggc
     8521 tggatccgtc aaggatccgt gcgtgcacaa aaaaacgcgc tttttctgcg cgcaggtgac
     8581 gggggaacag cccgcgtttc agggggtaaa tagcatcccc tgaacgatgt cgcagcgaca
     8641 caacagaatg gctgtatttc tcacgctgag cgtgaagaat acgtgaggga ttctgatttg
     8701 atggggtgaa aggtaaggcc gtcaaaatcg cactgaggcg gcgagaacat gcagtcaacg
     8761 cggtgggatt gcgtaagagt ctgaccgtcg atggtggcga taaactggaa ggcgtcgtga
     8821 aattatctga ctgatacagg agctggagag tcggggcata aattttttat gccccggcga
     8881 agcagcagac aagcgaagcg cgtcaggatg tgggctgggt gtctaacagt gcgtaagggt
     8941 taaagcggat cacctcttcg ccaagccagt cattgatgtg cttcatggcc tccatgacgg
     9001 gcatcagctc gttaattgcg taaacccgcg cggccttctc cacatcacca aacgcacttt
     9061 tttcacccgg catcgccccc atcagttgcg gcggaacgcg gtgcgcagcc agcacatcat
     9121 cacgggatgc cgccttaaca ttcatgaact catcctttgc ggtgatctgc tggaacggca
     9181 aaatttgcac cccctctttg cccccgttgg gcgcatgaat gagcacgttt ttaaacgcac
     9241 caccaccacg cgcaccctgt agcgtttctt tcagggagtc catgctttcg cggtttacct
     9301 gcgctgcacc gatgtagatg atgcacccgg cgtgggatcc gttgtcgtaa tacagttttc
     9361 tgaacatgtc cgccgaatga gacaggctgg ccgagagtaa tgcgccgaga tattccggca
     9421 tgccgtagat ttcctggtta atgtccggat tcatcaggtg gcacactttg cctgggcgaa
     9481 actgaaacgc atccttgcca tcctgcacat accaccatga ttcaagatcg cttccgcgtc
     9541 gcatgtattt cgccagggcg tgccgtaatt taagcggttc gccgagcata ttgcttcgaa
     9601 gctcaaggaa tgcgttaccg aacacaaacc agtccagcgc cagcgccgag aaatcctgcc
     9661 gggaaagcag cgggtgcggg atgtagcaac cgagcaatac attgcgctta aagtaaagcg
     9721 cagactgatg ccaggacgtt tgccgggcgg ctcttgccag accgtaccag tccaccgggg
     9781 tttcatacca ccgcccgtta tcagcacagt acatattgtc cagcaggtca tgcccggtca
     9841 ggcgataagg accatcaaat gtgaatgcac tgagcgatga ttctttcctg agcgcatcag
     9901 cgagatcaat gcgtgaactc atgcgcactt ttttattttt tctgctcatc agaactccat
     9961 aaccgtgaaa cgctcgtttt ctccttcgcc gccaatcggt tcgttaatga cagcaagcat
    10021 ggttgcccac gcaaggtcgc cgtggctgat ccccctcgcg cggtccgttt cgtaagtgat
    10081 aaagccgccc ggtgttttca ccttacgcac ggcgttaaag gccgcgacca gctcgcgttc
    10141 ggcgcgatcg tattcccacc gcccggcacg cattatttgc agcattttca gtaccagcga
    10201 ccgttttgat gacagcgtga aggtgtacgg aatagcagca gggaaaaacc gtttcactat
    10261 ctgataaaca gcctccccgt tcccgcccgt cacatcaatg ccgatgtgtt ccacgttgta
    10321 gcgatacgtg aactcttcaa tgactctggc ctgttcttca aactccagcc cctgaacgcg
    10381 tcgcgtctcc accgttcgaa aacggccacc aggaacagcc ggaggaacca ccacggacac
    10441 agcgccgctg tcgccgttgc cactactgcc gtttgcgtca tacccaatcc ataccggacg
    10501 attccccatc gggcggggag caaaaggttt ccagtctttc cagtcgtcgt atccgtcaac
    10561 accgcagcca atcaggatat tcaggttaaa tgccgattcc ccttcgcgga caaactcaca
    10621 catatagaga ttgaggaact cgtcttcggt gttttcatca cgaatttcgt cgatatcggt
    10681 gtgtttccag ccgtgattga ccacatcttc cagcgtgaca atttgccgcc acgtccggtc
    10741 agggcagata agcccgttat gcagcgtttt ccagtccaca gaaaaacgct ggcgtttatg
    10801 cgtggccttt ttctcgttcc agcggtcgcc gttccagtag gcgtatgcct cgtgcgtttc
    10861 ggtggatggc gtggagaagt aggtgcgccg cagtccgctg agggttgcca tagcgccagc
    10921 caccttgcgc agttcagcaa agcgactgac ccagaaaaat tcatcaaaat aaaaattgcc
    10981 cgtataggac tgtgccgacg cagcagaagt gccgagaaaa tgcagctctg cgccgttgga
    11041 gaggatgatt ttatcgcccc ctttcagctc cacatcaact tcagccgcgg ccttctgaat
    11101 aatgctttta aactggaacg cctgacgacg cgacgcagac aaaaaaatct ggttacgctg
    11161 gtaaggttgc gccacatcgt cacgcagcgc catcagcaga gcttcctgtg caaaatacca
    11221 ggtcgcccca atctgtcggg atttcaggat catcctgtta cgtatcccgg cttccctgca
    11281 aagggtcagg gagtcaaacc agccccgctg atgccactcc agcctgctga tgattttttc
    11341 ccgcagtgcg gcaatctgtt ccggcgtgaa atgatttttg agttttttcg cccggccttt
    11401 ctttcctgtg accgtcgcat ccggctggcc atcatgcagt tttttaagct gccgggtcag
    11461 caggtctatt tccttaaagt caccgcctgt tttattctgt ttttcagtaa gctggatgag
    11521 gcgcgcatcg atggactgcg tgacacgctg cacgggtggc gtttcatccc actggtcgcg
    11581 ttttttccac gcataaatcg tgttcgggtt tattcccatc agacgtgata tttctgcggg
    11641 cggataaccc tgccagtaaa gttgccgcgc acgctggcgc acaaaagcgt cctgaatcat
    11701 tgctccccct gagtaattac aggaagatta cccgcgcgcg aaactgttct ccttaacccc
    11761 ctgttctggc cgttttctta caacaaaatc cctttgtatc agcctgttac gctttgccat
    11821 catgactgaa gaaccagtca gaggggcaaa aactatggct aatgaaaaaa agacatcccg
    11881 caaaaagttt cgcgtggctg tctccggatc aactgttgat ggccgtgaaa tcagtccggt
    11941 gcatctgcgt gaagccgccg agaacttcaa cccggatgtt tacgctgccc gcgtgaacgt
    12001 tgagcactat ctctcgccat gcccgtcaag cgaattttcc gcaatgggcg atgtcaccgc
    12061 gctgagtacg gaagatatta cggaaggccc gctggccgga cgtactgcgc tgtatgcaga
    12121 aatcgaaccg accgagcgca tgaagcagct tgtcgctgac ggcaagaaaa tctattccag
    12181 tatcgaactg cacccgcagt tctccgttaa cgggcgtgcc tatctggtcg ggctggcgat
    12241 gaccgacacc ccggcaagcc tgggcactga gcgcctgaaa ttcacggcac agcagcgtca
    12301 ggcggtgatg acgttcaaca gtatccaggg tgaagcaccg cttatctccg aagccatcga
    12361 gtctgaaatc atcgaaatgg cagaacaacg ccaggaagaa ggcacccagt ggtttaaccg
    12421 cgtaatgggg attattggtc gtggccgcaa agcggatgac gccagtttct cccgtattca
    12481 ggaagcggtg gaaggcgttg caacatcaca ggccgacatt atcgaccgtt ttaatgtgct
    12541 ggaaacccgc catcagcagg accgccagaa aatcacgtca ctgaccacag agctgacagc
    12601 actgaaggaa aaactgcgca cgcaggacgg cgatccgcag aaccgcttca ccgcaacggg
    12661 cgcagcctcc gaccagctgg ctgacttctg ataagacaaa ggagcaaatt ttttatgaat
    12721 ctggtgatgt cagatattac ccgcaacaag ctgggttgct atatggcgca gcaggcgtcg
    12781 cttaacaata tcccggtatc tgcactggta tcgcgattta ccgtggaacc cgcggtgcag
    12841 cagcgttttg aaaacgcctc aaaggaaagt accgaattta cgaaaagaat taacgtgatc
    12901 ggcgtgaccg accagaaagg cgaaaaaatc ctcctggaca ccaccgggcc aattgcgcgc
    12961 acgaatagca gttatgacgg catcaaacgc cgtaacccga ataacgtgat cgatatgaag
    13021 tctcgtcagt accagtgcga acaggtgaac tacgatacct ttatttcgta cccacagctt
    13081 gatacctggg cggcccacag cgattttcag tcccgtatca gtacacagat cgcccggcag
    13141 gtagcgcttg atcgcatcat gattggcttt aacggcacat cccacgccta cgagtctgat
    13201 tttcacacca acaagctgct tcaggacgtt aacgtgggct ggctggagca catcagaacc
    13261 gatgccagcg agcgcgtaat gaatgacgtg acgctgacct cccgcaacat ggacaacact
    13321 gtggcgcacg cgggtaagta tgcgaatgcc gatgctctgg tacaggatgc gcgctcatcc
    13381 ctgctggatg aatggcacaa ggaagctgac gacctcgtgg tgattatggg gcgcaacctg
    13441 tttaactcgc tgcgtctgcc cgtgctgaac agcatcagcg gccagaatcc caatgcggaa
    13501 ttacttgccg ggcagctcat cctgtcatcg cgcaccattg gcgggctggg cgtgttcctt
    13561 gcgccgttct tcccggatgc aacgatgctt atcacctcgt tcaacaacct gtcgatttac
    13621 tggcagaaag gttcaatgcg tcgtctgatg aaagacgagc cggaatacaa ccgcatcgcc
    13681 acctaccagt ccatcaatga cgcttatgtc gttgaagact atggcaagtg cgcgatggtc
    13741 actggcctga agttcgccga cagctaatca actcacggcg ggcatcatgc ccgcctgtaa
    13801 cggagagaaa aaatgattac tcctgcacaa caacactggc agaacgtgat ggcacagcgc
    13861 gcaggccggg cgaatgaagg tgtggaccac gccgcgtgta ccgcgcatga agaagtgctg
    13921 tatcgtctgc gtctggcaca ggccaggcta aaggccatac aggccagaag cgcgaaagcc
    13981 gccatcaaaa aagagttgtt gccggacttt tccggctgga ttgagggaac gctggaggct
    14041 gacggcgggc aacaggacga agtaattgcc acgctgatgg tgtgggcgat tgactgcggc
    14101 gatcttccgc tggcgctgcg tattggtgca tatgtggtcc gtcacaacct catcatgccg
    14161 gataactttg gccgtactgc tgccacggta ctgaccgaag aaatctgcaa cccggtactg
    14221 acgcaggctg ggacggatgc cgacgcggat ttatccgcct ttatcgaacc actggacaca
    14281 ctttgggaaa ttgtcgccaa ccaggacatt ccggacgaag tgcgcgccaa attatgcaag
    14341 gcgtgtgcct ttgcccgtcg tggcctgacc gatgcagaca acatggcctc atcactgaag
    14401 ctgctgcgcg aagcgatgca cctgaacccg aacgcaggtg tgaaacgcga gattgcaacc
    14461 ctttcccgcg ccctgaaaaa agccgattcc gcagccgaac cagaagacgc cagcgcacag
    14521 caggcgcagg acgaaagcag caaaaataaa aagacaacgc ggaagcctgc aacacgaaaa
    14581 accaccgcga cgcagaaggc aaagcgcggt taacgactga ccccgtcagc gggcggcgtg
    14641 cgcggtgttc cggtttgact ccgtgaccgt ttacaccgcg cacccaccgc ccgatttttt
    14701 caggagtgaa ccccatgagt atggttgcca gaactaaccc cggccccgca gaggacgaca
    14761 tcaccgatac cgatgatggc gacactcgca tttcagcggg tgcattctgg ccggatattg
    14821 tgctgcgtga gctgcgtctg gcggtacgac tgccgggccg cgtgaccacc tcccgcctgc
    14881 tgcataccgc caccggggcc atagcacacg ttacccgcga gctggaagca tggcagcagg
    14941 aacaacaggc ggctggctat cagacgctgg ccgatgttcc ggcccctgta attaacggag
    15001 aaagcgtcaa tctctggcac tggcgcaatg ctgtttatac cgccacacgc gccctgattc
    15061 tggagcgtta ccgcgatgcg gacacaacgg acaagggcga ccgccgggcg gacgcactgg
    15121 atatacagac atcggatttg tggcgcgatg tgagctgggc catctctgac attctgggac
    15181 gaccgcgaat gtttgcggag ctgtgctgat gaaagtgaag gcactggaag gcgacaccgt
    15241 ggattcgctc tgtttccggt actacggcac gacacggggc gtcaccgaaa aggtgctgga
    15301 tgccaacccc ggactctgtc agcaggtatt tctggacgcc gggcaggaag tggagatgcc
    15361 ggagccggag aagaagaaac gagaaatgat tcagttgtgg ggggagtagc agtgagcacc
    15421 attcaaacag ggatcacaga gcaggttatt gcgtggctct ttgaccacct gccaacggtg
    15481 tatgcagtag gcgcggctgt cagcatttcc gcgctgatga gtctttatga cggacgaaca
    15541 ctggttcaga ccgtaacggg atcgctggcg tgcggcgttc ttgccatggc cgtggccggg
    15601 tcgctgcgct tcttcgggat ccctgaggat gcagtgacgt tttttggtgc ctcaatcggt
    15661 tttatgggcg cagagaaagc acgcgacaag gttattgcaa tatttgatcg cagggtgaag
    15721 gagaggaacg aatgagcaac acatttaaat tcagcagccg aagcgaaaag aatttgcagg
    15781 gtgtaaatcc tgatctggtg aaagtgaccc gacgggcgct ggaaatctcg gaagtggatt
    15841 ttggtatcac cgaaggattg cgcagtcgtt atcgtcagaa gcagctcgtg accacaggca
    15901 agagccagac catgaacagt cgccatctca cagggcatgc cgtggatgtt gtggcttatg
    15961 ttggcagcca ggtgtcatgg gaatggccgc tgtacgaaaa aatcgcagca gcattcagac
    16021 aggccagccg ggaactgaat attccggtgg aatggggcgg cgactggaag accctgaaag
    16081 acggaccgca ttttcagtta ccacacggag cctatccggc atgaagctct ggcccacgct
    16141 tggcgtcgct ttccttctga ttgccgcatg gggaacatcc atgcgtctgt cgtggtcgct
    16201 gggccgggag aacgccagaa acgaagcgca ggccagcacc ctgaaaagta ccgtcgacac
    16261 actgaatatc atcagcgccg gggtacagga tatgcagcag gtgctggctc aactccgcgc
    16321 ggacaatcag caacgcaatc aggacggaga ggtaagacgt gaacagctac gcaacgatat
    16381 tgcaaaagat gaatgcgccc acgctttgcc tgacgctcgt tttactgaca ggttgcgcag
    16441 gcacgcagaa cgcgccacgg ccagcgccgt cagtccggct tataccgcag acgctgacca
    16501 tgccggtaac gcctcccccc ttccctgacc aacccacatg gggaaacctc ggaatatggg
    16561 gcgaccgcct tctggatgca ctggaaacct gtaacgcgga taaacgggcc attgctgaac
    16621 tggataagag aatagccgaa ctgacacacc agacgggagt aacacaatga ccagtaagaa
    16681 ctttgcactg attacagcca tgacacaggc tgaactgact caaaaggtga atgaacatct
    16741 tgcgaaaggg tggcatcttc agggggagac gcgggttgcc tacgaacccg gcaccccgtg
    16801 gtatctaatg caggcaatgg tggccgatgg cactacagac atctcacctg attcccccca
    16861 gcacggcagc gtgccggagt ggtattacgt ggtggtactt gctggtcaat ccaatgccat
    16921 gtcatatggt gagggaatgc cgctgccgga ttcttacgat gcgccccacc cacgcattaa
    16981 gcaactggcc cgtcgcaaca cagtgactcc cggtggtaaa gcatgcgcat ttaacgacat
    17041 cattccggca gatcactgcc tgcatgatgt tcaggatatg agcgcactga atcacccgaa
    17101 tgcagacctg agcaaagggc agtatggctg tgtcggacag ggcttgcata ttgccaaacg
    17161 cctgttgcct tacattccac agaatgccgg gattttactg gttccatgct gtcgtggtgg
    17221 ttcggcattc acccagggcg cggaggggac attcagcgag tccacaggag ccagtcagga
    17281 ttcggctcgc tggggtgtgg gtaaaccttt atatcaggac ctgattttgc gcacgaaggc
    17341 cgcattgcag aaaaacccaa aaaacatgct gctggccgta tgctggatgc agggcgaatt
    17401 tgacatgagc gccgctacgt actcacagca acctccgctg tttgcggcca tgctgaaaca
    17461 gtttcgtgcg gacattaccg agtttaacac gcagtgtcat ggaggcagag cggcaagtgt
    17521 gccatggatt tgtggtgaca cgacgtatta ctggaaaaac acctacggca cgcagtacga
    17581 caccatttac ggggcgtaca aaaacaggga gagcgacaac gttttctttg tgccattcct
    17641 gaccgatggt agtggcaaca atacctccac caacgcacca acggaagatc cggatgctgc
    17701 aagtgaggga tattacggtt cggcatcccg aacgaacaaa aactgggtat catcaaatcg
    17761 ccagacgcat ttcagttcat gggcgcgcag gagcattatt ccggatcgta tggcaaccgc
    17821 tattctgaac gtagccggtc gcacct
//
LOCUS       DAZFWL020000093        17631 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFWL020000093 DAZFWL020000000
VERSION     DAZFWL020000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.093"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..119
                     /locus_tag="ACHKNP_RS16765"
                     /old_locus_tag="pgaptmp_003353"
     CDS             <1..119
                     /locus_tag="ACHKNP_RS16765"
                     /old_locus_tag="pgaptmp_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705469.1"
                     /translation="IFDNDEDKADSAALLPCIEQAREDLHTLRQLQLLQQNR"
     gene            204..764
                     /locus_tag="ACHKNP_RS16770"
                     /old_locus_tag="pgaptmp_003354"
                     /pseudo
     CDS             204..764
                     /locus_tag="ACHKNP_RS16770"
                     /old_locus_tag="pgaptmp_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            836..1840
                     /locus_tag="ACHKNP_RS16775"
                     /old_locus_tag="pgaptmp_003355"
     CDS             836..1840
                     /locus_tag="ACHKNP_RS16775"
                     /old_locus_tag="pgaptmp_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HGU4705470.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            2122..2403
                     /locus_tag="ACHKNP_RS16780"
                     /old_locus_tag="pgaptmp_003356"
                     /pseudo
     CDS             2122..2403
                     /locus_tag="ACHKNP_RS16780"
                     /old_locus_tag="pgaptmp_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(2553..2640)
                     /locus_tag="ACHKNP_RS16785"
                     /old_locus_tag="pgaptmp_003357"
     tRNA            complement(2553..2640)
                     /locus_tag="ACHKNP_RS16785"
                     /old_locus_tag="pgaptmp_003357"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2604..2606),aa:Ser,seq:gga)
     gene            complement(2894..3112)
                     /gene="infA"
                     /locus_tag="ACHKNP_RS16790"
                     /old_locus_tag="pgaptmp_003358"
     CDS             complement(2894..3112)
                     /gene="infA"
                     /locus_tag="ACHKNP_RS16790"
                     /old_locus_tag="pgaptmp_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGU4705471.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(3397..4101)
                     /gene="aat"
                     /locus_tag="ACHKNP_RS16795"
                     /old_locus_tag="pgaptmp_003359"
     CDS             complement(3397..4101)
                     /gene="aat"
                     /locus_tag="ACHKNP_RS16795"
                     /old_locus_tag="pgaptmp_003359"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HGU4705472.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(4143..5864)
                     /gene="cydC"
                     /locus_tag="ACHKNP_RS16800"
                     /old_locus_tag="pgaptmp_003360"
     CDS             complement(4143..5864)
                     /gene="cydC"
                     /locus_tag="ACHKNP_RS16800"
                     /old_locus_tag="pgaptmp_003360"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HGU4705473.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFRLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(5865..7631)
                     /gene="cydD"
                     /locus_tag="ACHKNP_RS16805"
                     /old_locus_tag="pgaptmp_003361"
     CDS             complement(5865..7631)
                     /gene="cydD"
                     /locus_tag="ACHKNP_RS16805"
                     /old_locus_tag="pgaptmp_003361"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HGU4705474.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(7754..8719)
                     /gene="trxB"
                     /locus_tag="ACHKNP_RS16810"
                     /old_locus_tag="pgaptmp_003362"
     CDS             complement(7754..8719)
                     /gene="trxB"
                     /locus_tag="ACHKNP_RS16810"
                     /old_locus_tag="pgaptmp_003362"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGU4705475.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGSGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            9263..9757
                     /gene="lrp"
                     /locus_tag="ACHKNP_RS16815"
                     /old_locus_tag="pgaptmp_003363"
     CDS             9263..9757
                     /gene="lrp"
                     /locus_tag="ACHKNP_RS16815"
                     /old_locus_tag="pgaptmp_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HGU4705476.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            9892..13998
                     /gene="ftsK"
                     /locus_tag="ACHKNP_RS16820"
                     /old_locus_tag="pgaptmp_003364"
     CDS             9892..13998
                     /gene="ftsK"
                     /locus_tag="ACHKNP_RS16820"
                     /old_locus_tag="pgaptmp_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGU4705477.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQSYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            14153..14764
                     /gene="lolA"
                     /locus_tag="ACHKNP_RS16825"
                     /old_locus_tag="pgaptmp_003365"
     CDS             14153..14764
                     /gene="lolA"
                     /locus_tag="ACHKNP_RS16825"
                     /old_locus_tag="pgaptmp_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HGU4705478.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            14775..16118
                     /gene="rarA"
                     /locus_tag="ACHKNP_RS16830"
                     /old_locus_tag="pgaptmp_003366"
                     /gene_synonym="mgsA"
     CDS             14775..16118
                     /gene="rarA"
                     /locus_tag="ACHKNP_RS16830"
                     /old_locus_tag="pgaptmp_003366"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HGU4705479.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            16209..17501
                     /gene="serS"
                     /locus_tag="ACHKNP_RS16835"
                     /old_locus_tag="pgaptmp_003367"
     CDS             16209..17501
                     /gene="serS"
                     /locus_tag="ACHKNP_RS16835"
                     /old_locus_tag="pgaptmp_003367"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGU4705480.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
BASE COUNT         4533 a         4475 c         4581 g         4042 t
ORIGIN      
        1 ttatctttga taacgatgag gataaagcgg attcggcagc actgctgccc tgcattgaac
       61 aggccaggga agatctccat accctgcgcc agttgcagct tctgcaacag aaccggtgag
      121 tcttacccac cagacttcat tccactgacg tcagcctgaa cagggctggc gttttcgttt
      181 ataaaccaaa agggaaaccg ataatgtcag acatcacaat ctcccgtccg gaagtggtca
      241 ccgggcatac ggacgttatc tgctcaacca gtatccgcca cattctggct gtacgaaaga
      301 gtacactgct gcaaatcgac acacttatcc gacaactggc agaaatctca gctatgacaa
      361 aaagcatcgg aggtaaaact gccctggact gggccatgaa acaggatttt cgctgcggct
      421 gctggctgat ggagaaaccg gaaacggcaa tgaaagccat tacccgcaat ctcgatcgcg
      481 aaatctggcg tgacctgatg caacgttcag ggatgctttc cttaatggat gcacaggccc
      541 gtgatacatg gtaccggtca ctggagtacg ataactttcc ggaaatcagt gaagcgaaca
      601 ttctgagcac atttgaacaa ctgcaccaga ataaggatga ggtgtttgag cgaggggtga
      661 tcaatgtttt caggggactg agctggaatt acaaaaccaa ttgcccctgc aaatttggca
      721 gtaaaattat cgtcaacaat ctggtgagat gggaccggtg gggataatga tatggtcacc
      781 cccaccttaa cagcagtacg ctgttaaggt aaatcccttt aacggagaga ccatcatgca
      841 gaacatcaca cttattggta tcgatctcgg aaagcactcc ttccacattc attgtcaaga
      901 caagtctggt aaggctcttc tgcgtaaaaa atttacccgt actaaactga tgcagtttct
      961 ggcgacatgt ccgccctcag ttgttgtaat ggaagcctgt gcgggtgcac actttatggc
     1021 tcgctgtatc tctgattttg ggcatgaaac taagctgatt tctccacagt tcgttcgccc
     1081 gtttgtcaaa agtaataaga atgattttgt tgatgcagaa gccatatgcg aagcggcttc
     1141 acgaccatca atgcgttttg tccagccaag aactgagacc cagcaggcca tgagggcgtt
     1201 acaccgggtc agagaatcgc tcatcagaga taaggtgaaa acaaccaacc agatccacgg
     1261 tttcttgctg gaatttggaa tcagcctgcc gataggtgat gctgtaataa aacggttgtc
     1321 tctcgtgttg gctgaacacg aaatccccga atatctgagc cgccttttgg tgaggttgca
     1381 tacccactac ttttaccttg ttgagcaaat agcagagctg gaatcagaat taagtcagtc
     1441 aatcaacgct gatgataccg ctcagcgcat tatgacaatt cctggtgttg gtccgattac
     1501 tgccagctta ctatcgtcgc aacttggtga cgggaaacag ttctcatgca gcagggattt
     1561 tgcagcttcc acaggacttg ttccccggca gtatagcact ggaggaaaat cgactctgtt
     1621 gggtatcagt aagcgaggtg ataaaaattt acgccggtta ctggttcagt gtgcccgctc
     1681 attcataatg cgacttgaac gcaagcatgg aaaacttgct gagtgggttc gggagcaact
     1741 aaacaagaag cattcgaatg tggttacctg cgctctggca aataaactgg ctcgaattgc
     1801 ctgggcaatc actactcaga aaaaagaata tcaggcctga tatagatgct gatataactc
     1861 aattagttta ttttaaacaa taagatacaa gttacctttc tggttttgcg aagactgatt
     1921 attgatgaca tgaacggcaa atcgacctga ttaaaaacct aacagatgaa atggctcact
     1981 gaagccgagg ggatttttag gattatcagg tgcggaactc atcggggcgc gggcatcctt
     2041 gcccacagag acgccggata gatttaagca agccaaccat taatcaaaaa tcagtgttgc
     2101 aaaaacgggg gtgaccatag atttcatctt atcaccggac aacaggctga ccgactcgcc
     2161 gatctggaaa gaatgttgca cctgttcagc ggtaaaccaa tccccgacaa ccgggaaaac
     2221 atcactattc gtctggatga tcacattcag tctgttcagg gtcaagagag ctatgaagat
     2281 gagatgttca gcatcagata ctttaggaag ggctccgcgc atatcacgtt cagaaaaccg
     2341 gaactggttg accgactgaa tgatattatt gcagaacact atcctgacat gctgtcagta
     2401 tagctttgcc agtatgctca aagatcggaa attgtccgat aagatgtgat cgcggctcat
     2461 acaaaccggt ttcagatggc atacccggtg gcatactgtc tggaaggata agtaaaaaag
     2521 cgcatattaa acaataacat gcgcttgtaa tctggcggtg agggggggat tcgaaccccc
     2581 gatacgttgc cgtatacaca ctttccaggc gtgctccttc agccactcgg acacctcacc
     2641 aaattgtttt gctaccaaac ctcatgggtg gcaacggggc gctactatag ggagttggag
     2701 taaaacggtc aagaagaatt ttaatgataa ttattgtttg ctcatactgt aaacaagttg
     2761 tgcagtatat ctacatcgag acaagttacg gacttatact tccaaagtac ttcatacata
     2821 tcacaaaata aaaaggccgg ttaaaccgac cttttactcg ttctttctct tcgcccatca
     2881 ggcggtaaaa caatcagcga ctacggaaga caatgcggcc tttgctcagg tcgtacgggg
     2941 tcagttcaac agtcactttg tcgcccgtca ggatgcggat gtagtttttg cgcattttac
     3001 cggagatgtg tgcagtaacc acgtgaccgt tttctaactc tacgcggaac atggtattag
     3061 gcaacgtttc aagaacggta ccttgcattt caatattgtc ttctttggcc atctaatcct
     3121 ctggggtatc actaccgtaa tttgaaccgg caagataatg ccgaagttct gtaaataagt
     3181 aaagatttgc gcgctaaatc gcaacaaaca ggttcggcac attactccga aaacacacgg
     3241 ctaagccgca ccaaaagcgc aacgtataag ggagcggtga gataaacgat gggcgttacc
     3301 tgacgcgaaa aaatccttat cggcagcggg gtaatgagcg taaccaactc tgcgaccgca
     3361 attataacac tctggggaga aatgtgccga aaacattcat tcttgtggtg aaaacaagca
     3421 tcgtggtacc cagaaattat tcggcaatcg tccgaggcgc atttgattga gataattaag
     3481 gtaatcccgg cggggaattt cgcaggcacc aagcgatgct gtgtgatcgt taaggacctg
     3541 gcagtcgata agcttaccgc catgaccgat aaattcctca cagaatacca gaagcgccgt
     3601 tttagacgca ttttccatcc ggctgaacat ggactcgcca caaaatagcg ttccctgggc
     3661 cacgccgtac ataccgccga caagctcatc ttcacgccag acttcaatgg agtgggcatg
     3721 gccgagttcg tgaaggcgat ggtaggcttc gaccacgcca cgcgtgatcc aggttccttc
     3781 ttcgcgatcg ctggcacagc cttcaatgac ctgaccaaaa gcgtaattca tcgtgacacg
     3841 atagggcgag cgtttatgaa atcgcttcat actacggctg atatgcagtg attctggcca
     3901 tagcaccgcg cggggatcgg gcgaccacca gaggatgggg tcgcctggag aaaaccacgg
     3961 aaaaatacca cgctggtaag ccattaacag gcgcgcaggg ctaagatcgc ccccaagtgc
     4021 cagcaggccg ttaggctcac gtaatgcgcc ttccggggaa gggaaggcta ttgaatggcg
     4081 agaaagctga accaggcgca tgaccgcaaa actccacgca agtcggatcg ttcaataata
     4141 gcttacaaac cctgcttgaa ctggtaataa cgcccctgtc tggcaagcag ctctgcgtga
     4201 gtaccttgct caataatttg cccgttgtcc atcactatta tttgttggaa acgagagagt
     4261 ccgcgaagtc gatgggtgac cattaacacc gttttctcac gcatcatttc tgcaagcaat
     4321 tcaaggatct ggctttcggt tgtggcatct aagccttcgg taggttcatc cagcaacacc
     4381 agtggcgcat catgtaacag cgcacgggcg atagccagac ggcgcagttc accaccggag
     4441 agctggcgtc cgccttcacc taaccaactg ttgagacctg catcctcgag cagcttctcc
     4501 agaccaacgc gacgcaagct ctccgccaga gcctcatcac tactgccagg cgaggcgagt
     4561 aacagattat cacgcagcgt ggcgctaaac agatgcactc gctgaggaac aacgctgatg
     4621 gtctgtcgta gagccgcttc attcaggctg gctatggggc tatcgttaag caaaatctct
     4681 ccctgttgcg ggtcccaagc gcgggtcagc agttgtaaca gtgttgattt gccgcatccg
     4741 gttcgcccga gaatcgcaat atgttccccg gcgtttacct gaagagaaat ccctttaagt
     4801 gcctgttggg attgctccgg ataagtgaac tgaacatccc gtaatgtcag cgaaacgcga
     4861 tcggcaacac gagtttgggt atcagggaag gtgacttccg gtttttgatc cgttaaatca
     4921 gtgatccgaa cggcagaggc aatgacttgc cccagatgct gaaatgcacc cgttactggt
     4981 gccagtgctt caaacgcggc taacgcgcag aagacaaaca gggcaattaa cgcgccgggt
     5041 tgagcattgc cgccaacgcc gccagacgcc atccacagca tcaggatcac cgctaacgcg
     5101 ccaatgagca gcattatcgc ttgcgacaat gcggtcagtt cagattgacg gcgttgcgct
     5161 tccagccatt gaatttctgt attctctagt tgcgtgcgat aacgatcgct ggcgccaaaa
     5221 atggtcagct cagcttgccc ttgcagccag gccgtcagtt gttggcgata ctgtccgcga
     5281 agatgagtca gattttgccc ggtgcttttt cccgcacgat aaaacagcgg tggcatcagg
     5341 aaaagcgtca gtaacataat gccgcccagc ctaaaggcga gggtgaaatc aaggaaactt
     5401 aacccgattg tcaccaccat aatcaccaca aaagcgccca ccagcggcga gataacgcgc
     5461 agataaagat gatcgagcgt atcaacatcc gccaccacgc gattgagcaa ttcgccctga
     5521 cgatagcgcg ccagtccggc aggggagagg ggcagcaatt tgctgaaggt gtaaatgcgc
     5581 agatgctgca acacgcggaa agtcgcgtcg tgacttacca gacgttcaaa ataacgcccg
     5641 gcagtacggg tgattgctgc gccacgcacg cccgcagcgg gtagcatata gttgaagctg
     5701 tacagtccgg caacccccgc aaccgctgag gccgagagga accagccgga aagtgtcaac
     5761 agaccgatac tggcgagcag cgtgacaatt gccagcacga taccaagact taacatccat
     5821 ttatgacgtt tatacagtgc cagatagggt agcaaagcgc gcatttaaat ctcctcctga
     5881 cgatgggcca gtaatgtggc gaatgggcca ccagccacac ttaattccgc gtaacgtcct
     5941 tgctcaataa tccggccatc ctgcataacc caaatgacat cccagtcagc aagatcttct
     6001 aactggtggg tgaccattaa cgttgtctgg cgcagagagg cggcattcag cgcctccatt
     6061 acgcgctgtt cactgtgagc atcaaggctg gcagcgggtt catccaacag taatagcgaa
     6121 cagggattta gtaacgcacg ggccaccgcc acgcgctgcg cctgccccac ggaaaggcgg
     6181 gcagcctggt cgccaacagg tgtatcaacg ccttgtggca ggagcggtag aaactcgctg
     6241 acccaggcgt tatccagcgc tgcttgtaat tcttgttcgc tggcatcagg tcgcgccagt
     6301 agtacgttat cccgcaatgt tgctgccggt aattgtgggt tttgcccaac ccaggagaga
     6361 tgtttacgcc atgattctgg tgataaatcg cgtaattcta tcccgttgat tcgtaatgat
     6421 ccctgatatg agagaaaacc agaaagcgcg ttcagcagtg agcttttacc tgaaccgctg
     6481 cgaccaacca acactgcacg ttggcctgct ggcaaagtaa agttcagcgg tccggccagc
     6541 gttttacctt ccggcgacgt gataaacaga tcctcggcct caatggtcac cggatcggtc
     6601 gatgctaatt ccacctcgcc acgctgcgga tgggcgagcg gggtttccat aaacgttttc
     6661 agactgtcag ctgcaccaac agcctgggct ttagcatgat aaaacgtacc gagatcgcgc
     6721 aatggctgga aaaactctgg cgcaaggatc agggccagaa aacccgcagc cagcgtcaca
     6781 ccggtatcgt agtgaccaaa atccagctcg ccgagatagg aaaaaccaaa gtagaccgcc
     6841 accagagcaa ttgacagcga ggtaaaaaat tcgagaatgc cggaggataa aaacgccagc
     6901 cgtagcactt ccattgtccg ttggcggaaa tcttccgaag cagaacgaat actttcaatt
     6961 tcagcttcac cacgaccaaa aatacgcaat gtttccatgc cgcgcaggcg atcgaggaaa
     7021 tgcccactta agcgagcaag agcgagaaag ttacgccggt tagcatcggc agcccccatc
     7081 ccaaccagcg ccataaacaa cggaattaac ggtgctgtgc ccagcagaat gagcgccgca
     7141 gcccagttag aggggaagat cgccaccaca atcagcaacg gcaccgacac tgccagcgcc
     7201 atttgcggca ggtagcgtgc atagtaatca tgcatatcgt caatttgctc gagtaccaac
     7261 gtcgcccagc tccccgcagg tttaccctga atccacgctg gccctgcttg ttgcagacgg
     7321 tcgagaacct gacggcggat ggcaaagcgg atatgctgcc cggcgtgata acccacccgt
     7381 tcgcgtaacc agaccaccca tgcgcgcagt acaaaggtca gaaccagtaa cgtaaaggga
     7441 agcagcaggg cttcacgggg aatattctcc ataatcatat gttgcagaat tcgcgccatg
     7501 aaccaggcct gggcaatgat caatatgccg cttacaaagc ccagcagacg agaaatattc
     7561 agccaacgtt gggagatgac gctttgctgt tttaaccagc gggttaactc tttttgacgg
     7621 gatttattca ttgcacgctt agcaggtgag ttatcagaat tatttgcaga gcaatgttac
     7681 aacggggaaa aaataaaggc gacccatagt cgcatggtgt cgccttcttt acttttgtta
     7741 ctgatttgta aaattatttt gcgtcagcta aaccatcgag gtagcgttcc gcatcaagtg
     7801 ctgccatgca gcctgtaccg gccgaagtaa tggcctgacg ataaatatga tccatcacgt
     7861 cacccgcggc aaagacgcca ggaatgctgg tctgggtggc attaccatga atacccgact
     7921 gcactttgat gtagccgttt tccagttcca gctgcccttc gaaaatggca gtattcgggc
     7981 tgtgaccgat agcaacaaac agaccagcaa cgtcgagtga ctcgatgtta tcgctgtttt
     8041 gcgtatcgcg cagacgaacg ccagtgacac ccatttgatc gccggtcact tcttccagcg
     8101 tacggttggt gtgcagaatg atgttgccgt tctccacttt atccatcaga cgcttaataa
     8161 ggattttttc cgcgcggaaa ccgtcacggc ggtgaataag atgcacttcc gaagcgatgt
     8221 tagacagata cagcgcctct tcaaccgcgg tattgccgcc gccgatgacc gcaactttct
     8281 ggttgcgata gaagaaaccg tcgcaggttg cacaagcaga aaccccactg cctttaaagg
     8341 cttcttcaga gggcaggccg agatagcgtg cagaagctcc ggtggcaata atcagcgcgt
     8401 cgcaagtgta ttcgccgtta tcgccattca gacggaacgg acggttttgc agatccacct
     8461 tgttgatatg atcaaaaatg atctcagttt caaacttggt ggcatgttcg tgcatgcgct
     8521 ccattaataa cggaccggtc agatcgtttg gatcgccagg ccagttttcc acttccgtgg
     8581 tggtggtcag ttggccgcct ttttccatgc cggtaatcag cacaggttgc aggttggcgc
     8641 gcgccgcgta gacagcagcg gtgtatcccg ccgggcctga acccaggata agcagtttac
     8701 tgtgtttggt cgtgcccatg agatccccat agttgttggc agacaatggg caggattgta
     8761 gggaatttac agacgtaaaa aaagagtatg acgattttgt taacaatttg tgcaatcggc
     8821 agcatcgata agcaggtcaa attctcccgt cattatcacc tctgctactt aaatttcccg
     8881 ctttataagc cgattaaatg atgaataaac gcccctgtta atgaatatct ggcatgttgt
     8941 actaaaaatc gatgttttgc tttgacaatc ccctggtgtt ttgcgaaaac attcgaggaa
     9001 gaaaaaaaca gtattcttat atgcgcataa ccatgcatgt aaataccatg tttaccgtgc
     9061 tagtgaaatc tacgtatggc gtggacagac gccattcgtg atgtcgatag ctgccacaag
     9121 gcaacggtct tctcaccgta gacccaggca ttgcgcgccg tgaatcttca tgatttcggt
     9181 ctatcgtgac gggtagcgac tctgaacagt gatgtttcag ggtcagacag gagtagggaa
     9241 ggaatacaga gagacaataa taatggtaga tagcaagaag cgccctggca aagatctcga
     9301 ccgtatcgat cgtaacattc ttaatgagtt gcaaaaggat gggcgtattt ctaacgtcga
     9361 gctttctaaa cgtgtgggac tttccccaac gccgtgcctt gagcgtgtgc gtcggctgga
     9421 aagacaaggg tttattcagg gctatacggc gctgctgaac ccccattatc tggatgcatc
     9481 acttctggta ttcgttgaga ttactctgaa tcgtggcgca ccggatgtgt ttgaacaatt
     9541 caataccgct gtacaaaaac ttgaagaaat tcaggagtgt catttagtat ctggtgattt
     9601 cgactacctg ttgaaaacac gcgtgccgga tatgtcagct taccgtaagt tgctggggga
     9661 aaccctgctg cgtctgcctg gcgtcaatga cacacggaca tacgttgtta tggaagaagt
     9721 caagcagagt aatcgtctgg ttattaagac gcgctaacac ggaacaggtg caaaatcggc
     9781 gtattttgat tacactcctg ttaatccata cagcaacagt actggggtaa cctggtactg
     9841 ttgtccgttt tagcatcggg caggaaaagc ctgtaacctg gagagccttt cttgagccag
     9901 gaatacactg aagacaaaga agtcacattg acaaagttaa gtagcggccg ccgccttctg
     9961 gaggcgttgc tgatccttat tgtcctgttt gccgtctggt tgatggctgc cttactaagc
    10021 tttaaccctt cggaccccag ctggtcgcaa acggcctggc atgaacctat ccataattta
    10081 ggtgggatgc ccggtgcgtg gttggcagat acgctgttct ttatttttgg cgtgatggct
    10141 tacaccattc ccgtcattat tgtcggcggt tgttggtttg cctggcgtca tcagtccagc
    10201 gacgaataca ttgattattt tgccgtttcg ctacgcatca ttggcgtttt ggcgctcatc
    10261 cttacctcct gtggtctggc ggcaatcaac gctgacgata tctggtattt tgcctccggt
    10321 ggcgtcattg gcagcttact aagtactacg ctacaaccac tgctacacag tagcggggga
    10381 actattgcgc tgctctgcgt ttgggctgcg ggcctgacgc tgttcaccgg ttggtcatgg
    10441 gtgaccattg ctgaaaaact cggcggctgg attttaaaca ttctcacctt cgccagtaat
    10501 cgtacccgtc gcgatgatac ctgggtcgat gaagatgaat atgaagatga cgaagagtat
    10561 gaagatgaaa accacggcaa acagcatgaa tcacgccgtg cccgtattct tcgcggcgcg
    10621 ctagcgcgtc gtaaacggtt ggcggaaaaa ttcattaatc cgatggggcg gcaaacagac
    10681 gctgcgttgt tctccggtaa gcggatggat gatgacgaag agattaccta cactgcacgc
    10741 ggtgtggctg ccgacccgga cgacgtccta ttttcgggca atcgtgcaac gcagccagaa
    10801 tatgacgaat acgatccatt attaaacggt gcgccaatta ccgaacctgt cgctgtagca
    10861 gctgctgcta ccacggcgac acaaagctgg gctgcgccgg ttgaacctgt gactcagacg
    10921 ccgcctgttg cctctgttga tgttccaccc tcgcaaccta cagtagcctg gcagcctgta
    10981 ccgggtccac aaacgggaga gccggttatt gctcctgcac cggaaggtta cccacagcag
    11041 tcacaatatg cgcagcctgc agtgcaatat aatgagccgc tgcaacaacc agtacagccg
    11101 cagcagtcgt attatgcacc tgcagctgaa caacctgcgc aacaacctta ctacgctcct
    11161 gcggcggaac aacctgttca gcaaccgtat tacgcaactg cgccggaaca gcctgcacaa
    11221 cagccgtatt acgcccctgc gccagaacaa ccggtggcag gtaacgcctg gcaagccgaa
    11281 gagcagcaat ccacttttgc tccacagtct acataccaga ctgagcaaac ttatcagcaa
    11341 ccagccgctc aggagccgtt gtaccaacag ccgcaatccg ttgaacagca gcctgttgtg
    11401 gagcctgaac ccgttgtaga agagacaaaa cccgcgcgtc cgccgcttta ctattttgaa
    11461 gaagtggaag agaagcgagc ccgtgaacgt gaacaacttg cggcctggta tcaaccgatt
    11521 ccagaaccgg ttaaagaacc agaaccgatc aaatcttcgt tgaaagcgcc ttctgttgca
    11581 gcagtacctc cagtagaagc cgctgccgct gtttctccgc tggcatctgg cgtgaaaaaa
    11641 gcgacactgg cgacgggggc tgccgcaacc gttgccgcgc cagtcttcag tctggcaaat
    11701 agcggtggac cgcgtccgca agtcaaagag gggattggcc cgcagttgcc acgaccgaaa
    11761 cgtatccgcg tgccaactcg tcgtgaactg gcgtcttacg gtattaagct gccctcacag
    11821 cgtgcggcgg aagagaaagc ccgtgaagcc cagcgcaatc agtatgattc tggcgatcag
    11881 tataacgatg atgaaatcga tgcgatgcag caggatgaac tggcacgtca gttcgcccag
    11941 acacagcagc aacgctatgg cgaacagtat caacatgatg tgcccgtaaa cgcagaagat
    12001 gcagatgctg cggcagaggc tgaactggct cgtcagtttg cgcaaactca acaacaacgt
    12061 tattccggcg aacaaccggc tggggcgaat ccgttctcgc tggatgattt tgaattttcg
    12121 ccaatgaaag cgttgctgga tgatggtcca cacgaaccgt tgtttacgcc aattgttgaa
    12181 cctgtacagc agccgcaaca accggttgca ccgcagcagc agtatcagca gccgcaacaa
    12241 ccagttccgc cacagcagca atatcagcag ccgcaacagc cggttgcgcc gcagccgcag
    12301 tatcagcagc cacaacagca ggttgcgccg cagccacaat atcagcagcc gcaacaaccg
    12361 gttgcgccac agccgcaata tcagcagccg caacaaccag ttgctccgca gccgcagtat
    12421 caacagccgc aacaaccggt tgcaccgcag cagcaggata ccctgcttca tccgctgttg
    12481 atgcgtaatg gcgacagccg tccgttgcat aaaccgacga cgccgctgcc ttctctggat
    12541 ttgctgacac cgccgccgag cgaagtggag ccggtagata cctttgcgct tgaacaaatg
    12601 gctcgcctgg tggaagcgcg tctggctgat ttccgtatta aagccgatgt cgtcaattac
    12661 tctccggggc cggttatcac tcgctttgaa cttaacctgg ctcctggcgt aaaagcggcg
    12721 cgcatttcta acttgtcacg ggaccttgcc cgttcacttt cgacggtggc ggtgcgtgtc
    12781 gttgaagtta ttcctggcaa accctatgta ggtctggagt taccaaataa aaaacgacaa
    12841 accgtttatc tgcgcgaagt tttggataac gccaaattcc gcgataatcc gtcgccatta
    12901 accgtggtgc tgggtaaaga tatcgccggt gagccggtgg ttgccgatct ggcgaaaatg
    12961 ccgcacttgt tggttgcggg gactaccggt tccggtaaat ctgtcggtgt gaacgcgatg
    13021 atcctgagca tgctttataa agcacagcca gaagatgtgc gtttcatcat gatcgacccg
    13081 aaaatgctgg agctttcggt ttatgaaggc attccgcatc tgttaacgga agtcgttact
    13141 gatatgaaag atgccgccaa cgcgctgcgc tggtgtgtta acgagatgga gcgtcggtat
    13201 aaactgatgt ctgcgctggg tgtgcgtaat ctggcgggtt ataacgaaaa aattgctgaa
    13261 gccgatcgca tgatgcgtcc gattccagac ccgtactgga agccgggtga cagtatggat
    13321 gcccagcatc cggtgctgaa aaaagaacca tacattgtgg tgttggttga cgaatttgcc
    13381 gacctgatga tgacggtagg taaaaaagtg gaagagctga tagcacgtct ggcgcaaaaa
    13441 gcccgtgccg cgggtatcca cctcgtactg gcaactcagc gtccatcggt tgatgttatt
    13501 actggtctga ttaaagcgaa tattccgacc cgtatcgcct ttaccgtatc cagtaagatt
    13561 gactcacgta ccattcttga tcaggctggc gcggaatcac tgctggggat gggggatatg
    13621 ctctactctg ggccgaactc cacgttgccg gtacgtgtcc atggtgcttt tgttcgcgat
    13681 caggaagttc atgccgtggt gcaggactgg aaagcgcgtg gtcgcccaca gtatgttgat
    13741 ggcatcactt ccgacagtga aagcgaaggt ggcgcgggcg gtttcgatgg cgctgaagaa
    13801 ctggatccgt tgttcgatca ggcggtgcag tttgtcactg aaaaacgcaa agcgtcaatt
    13861 tctggcgtac agcgtcagtt ccgcattggt tataaccgtg cagcgcgtat tatcgaacag
    13921 atggaagcgc aggggattgt cagcgaacag gggcacaacg gtaatcgtga agtgctggcc
    13981 ccaccgccgt ttgactaatt aatgcattgc cggataaggc gcggtagcgt cgcatccggc
    14041 actctatcaa ctgaaaattc agtattttct tctttcctca agctgattat tagcctggaa
    14101 tagagagtag agggaactcc cgatcgggag tgacgtaatt tgaggaataa tgatgaaaaa
    14161 aattgccatc acctgtgcat tactctcaag cttagtagca agcagcgttt gggctgatgc
    14221 cgcaagcgat ctgaaaagcc gcctggataa agtcagcagc ttccacgcca gcttcacaca
    14281 aaaagtgact gacggtagcg gcgcggcggt gcaggaaggt cagggcgatc tgtgggtgaa
    14341 acgtccaaac ttattcaact ggcatatgac acaacctgat gaaagcattc tggtttctga
    14401 cggtaaaaca ctgtggttct ataacccgtt cgttgagcaa gctacggcaa cctggctgaa
    14461 agatgccacc ggtaatacgc cgtttatgct gattgcccgc aaccagtcca gcgactggca
    14521 gcagtacaat atcaaacaga atggcgatga ctttgtcctg acgccgaaag ccagcaatgg
    14581 caatctgaag cagttcacca ttaacgtggg acgtgatggc acaatccatc agtttagcgc
    14641 ggtggagcag gacgatcagc gcagcagtta tcaactgaaa tcccagcaaa atggggctgt
    14701 ggatgcagcg aaatttacct tcaccccgcc gcaaggcgtc acggtagatg atcaacgtaa
    14761 gtagaggcac ctgagtgagc aatctgtcgc tcgatttttc ggataatact tttcaacctc
    14821 tggccgcgcg tatgcggcca gaaaatttag cacagtatat cggccagcaa catttgctgg
    14881 ctgcggggaa gccgttgccg cgcgctatcg aagccgggca tttacattct atgatcctct
    14941 gggggccacc gggtaccggc aaaacaaccc tcgctgaagt gattgcccgc tatgcgaacg
    15001 ctgatgtgga acgtatttct gccgtcacct ctggcgtgaa agagattcgc gaggcgatcg
    15061 agcgcgcccg gcaaaaccgc aatgcaggtc gccgcactat tctttttgtt gacgaagtgc
    15121 accgattcaa caaaagccag caggatgcat ttctgccaca tattgaagac ggcaccatca
    15181 cttttattgg cgcaaccact gaaaacccgt cgtttgagct taattcggca ctgctttccc
    15241 gtgcccgtgt ctatctgttg aaatccctgg gtacagagga tattgagcaa gtactaactc
    15301 aggcgatgga agacaagacc cgtggttatg gtggtcagga tattgttctg ccagatgaaa
    15361 cacgacgcgc cattgctgaa ctggtgaatg gcgacgcgcg ccgggcgtta aatacgctgg
    15421 aaatgatggc ggatatggcc gaagtcaatg atagcggtaa gcgggtcctg aagcctgaat
    15481 tattgaccga aatcgccggt gagcgaagcg cccgctttga taacaaaggc gatcgctttt
    15541 acgatctcat ttccgcactg cataagtcgg tacgtggtag cgcacccgat gcggcgctct
    15601 actggtatgc gcgaattatt accgctggtg gcgatccgtt atatgtcgcg cgtcgttgcc
    15661 tggcaattgc ttccgaagat gtcggcaatg ccgacccacg ggcgatgcag gtggcaattg
    15721 cggcctggga ttgctttact cgcgttggcc cggcggaagg tgaacgcgcc attgctcagg
    15781 cgattgttta cctggcctgc gcgccaaaaa gcaacgctgt ctacactgcg tttaaagctg
    15841 cgctggcgga tgctcgcgaa cgtccggatt atgacgtgcc ggttcatttg cgtaatgcgc
    15901 cgacgaaatt aatgaaggaa atgggctacg ggcaggaata tcgttacgct catgatgaag
    15961 caaacgctta tgctgccggt gaggtttact tcccgccgga aatagcacaa acacgctatt
    16021 atttcccgac aaacaggggc cttgaaggca agattggcga aaagctcgcc tggctggctg
    16081 aacaggatca aaatagcccc ataaaacgct accgttaatg ttatcgttgc ggtaatgttg
    16141 ttactgtatc cctgtggtcg caggctgtgg ccacatctcc catttaattc gataagcaca
    16201 ggataagcat gctcgatccc aatctgctgc gtaatgagcc agacgcagtc gctgaaaaac
    16261 tggcacgccg gggctttaag ctggatgtag ataagctggg cgctcttgaa gagcgtcgta
    16321 aagtattgca ggtcaaaacg gaaaacctgc aagcggagcg taactcccga tcgaaatcca
    16381 ttggccaggc gaaagcgcgc ggggaagata tcgagccttt acgtctggaa gtgaacaaac
    16441 tgggcgaaga gctggatgca gcaaaagccg agctggatgc tttacaggct gaaattcgcg
    16501 atatcgcgct gaccatccct aacctgcctg cagatgaagt gccggtaggt aaagacgaaa
    16561 atgacaacgt cgaagtcagc cgctggggca ccccgcgtga gtttgacttt gaagttcgtg
    16621 accatgtgac gctgggtgaa atgcactctg gcctcgactt tgcagctgca gttaagctga
    16681 ctggttcccg ctttgtggta atgaaagggc agattgctcg catgcaccgc gcactgtcgc
    16741 agtttatgct ggatctgcat accgaacagc atggctacag tgagaactat gttccgtacc
    16801 tggttaacca ggacacgctg tacggtacgg gtcagctgcc gaaatttgct ggcgatctgt
    16861 ttcatactcg tccgctggaa gaagaagcag acaccagtaa ctatgcgctg atcccaacgg
    16921 cagaagttcc gctgactaac ctggtgcgcg gtgaaatcat cgatgaagat gatctgccaa
    16981 ttaagatgac cgcccacacc ccatgcttcc gttctgaagc cggttcatat ggtcgtgaca
    17041 cccgtggtct gatccgtatg caccagttcg acaaagttga aatggtgcag atcgtgcgcc
    17101 cggaagactc aatggcggcg ctggaagaga tgactggtca tgcagaaaaa gtcctgcagt
    17161 tgttgggcct gccgtaccgt aaaatcatcc tttgcactgg cgacatgggc tttggcgctt
    17221 gcaaaactta cgacctggaa gtatggatcc cggcacagaa cacctaccgt gagatctctt
    17281 cctgctccaa cgtttgggat ttccaggcgc gtcgtatgca ggcacgctgc cgcagcaagt
    17341 cggacaagaa aacccgtctg gttcataccc tgaacggttc tggactggct gttggtcgta
    17401 cgctggttgc agtaatggaa aactatcagc aggctgatgg tcgtattgaa gtaccagaag
    17461 ttctgcgtcc gtatatgaac ggactggaat atattggcta atacccaatt tttctgaatc
    17521 taaaaagcgc ctgcgggcgc tttttttgtc tccctttgat accgaacaat aattactcct
    17581 cacttacacg taatactact ttcgagtgaa aatctaccta tctctttgat t
//
LOCUS       DAZFWL020000094        17211 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFWL020000094 DAZFWL020000000
VERSION     DAZFWL020000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.094"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            12..596
                     /locus_tag="ACHKNP_RS16840"
                     /old_locus_tag="pgaptmp_003368"
     CDS             12..596
                     /locus_tag="ACHKNP_RS16840"
                     /old_locus_tag="pgaptmp_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4705481.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            676..1371
                     /locus_tag="ACHKNP_RS16845"
                     /old_locus_tag="pgaptmp_003369"
     CDS             676..1371
                     /locus_tag="ACHKNP_RS16845"
                     /old_locus_tag="pgaptmp_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4705482.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1401..3917
                     /locus_tag="ACHKNP_RS16850"
                     /old_locus_tag="pgaptmp_003370"
     CDS             1401..3917
                     /locus_tag="ACHKNP_RS16850"
                     /old_locus_tag="pgaptmp_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4705483.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQNLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3928..5019
                     /locus_tag="ACHKNP_RS16855"
                     /old_locus_tag="pgaptmp_003371"
     CDS             3928..5019
                     /locus_tag="ACHKNP_RS16855"
                     /old_locus_tag="pgaptmp_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4705484.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5062..5922)
                     /gene="rsmI"
                     /locus_tag="ACHKNP_RS16860"
                     /old_locus_tag="pgaptmp_003372"
     CDS             complement(5062..5922)
                     /gene="rsmI"
                     /locus_tag="ACHKNP_RS16860"
                     /old_locus_tag="pgaptmp_003372"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGU4705485.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5986..8022
                     /gene="lpoA"
                     /locus_tag="ACHKNP_RS16865"
                     /old_locus_tag="pgaptmp_003373"
     CDS             5986..8022
                     /gene="lpoA"
                     /locus_tag="ACHKNP_RS16865"
                     /old_locus_tag="pgaptmp_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HGU4705486.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7980..8375
                     /locus_tag="ACHKNP_RS16870"
                     /old_locus_tag="pgaptmp_003374"
     CDS             7980..8375
                     /locus_tag="ACHKNP_RS16870"
                     /old_locus_tag="pgaptmp_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HGU4705487.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8395..8985
                     /gene="diaA"
                     /locus_tag="ACHKNP_RS16875"
                     /old_locus_tag="pgaptmp_003375"
     CDS             8395..8985
                     /gene="diaA"
                     /locus_tag="ACHKNP_RS16875"
                     /old_locus_tag="pgaptmp_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HGU4705488.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            8995..9570
                     /gene="dolP"
                     /locus_tag="ACHKNP_RS16880"
                     /old_locus_tag="pgaptmp_003376"
     CDS             8995..9570
                     /gene="dolP"
                     /locus_tag="ACHKNP_RS16880"
                     /old_locus_tag="pgaptmp_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HGU4705489.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9684..10724)
                     /locus_tag="ACHKNP_RS16885"
                     /old_locus_tag="pgaptmp_003377"
     CDS             complement(9684..10724)
                     /locus_tag="ACHKNP_RS16885"
                     /old_locus_tag="pgaptmp_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGU4705490.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMLYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10797..11432)
                     /locus_tag="ACHKNP_RS16890"
                     /old_locus_tag="pgaptmp_003378"
     CDS             complement(10797..11432)
                     /locus_tag="ACHKNP_RS16890"
                     /old_locus_tag="pgaptmp_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU4705491.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11560..12078
                     /gene="yhbO"
                     /locus_tag="ACHKNP_RS16895"
                     /old_locus_tag="pgaptmp_003379"
     CDS             11560..12078
                     /gene="yhbO"
                     /locus_tag="ACHKNP_RS16895"
                     /old_locus_tag="pgaptmp_003379"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HGU4705492.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12058..12501)
                     /locus_tag="ACHKNP_RS16900"
                     /old_locus_tag="pgaptmp_003380"
     CDS             complement(12058..12501)
                     /locus_tag="ACHKNP_RS16900"
                     /old_locus_tag="pgaptmp_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HGU4705493.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12552..12854
                     /gene="yhbQ"
                     /locus_tag="ACHKNP_RS16905"
                     /old_locus_tag="pgaptmp_003381"
     CDS             12552..12854
                     /gene="yhbQ"
                     /locus_tag="ACHKNP_RS16905"
                     /old_locus_tag="pgaptmp_003381"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HGU4705494.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12841..13344)
                     /locus_tag="ACHKNP_RS16910"
                     /old_locus_tag="pgaptmp_003382"
     CDS             complement(12841..13344)
                     /locus_tag="ACHKNP_RS16910"
                     /old_locus_tag="pgaptmp_003382"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4705495.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13338..13862)
                     /locus_tag="ACHKNP_RS16915"
                     /old_locus_tag="pgaptmp_003383"
     CDS             complement(13338..13862)
                     /locus_tag="ACHKNP_RS16915"
                     /old_locus_tag="pgaptmp_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HGU4705496.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14071..15066
                     /locus_tag="ACHKNP_RS16920"
                     /old_locus_tag="pgaptmp_003384"
     CDS             14071..15066
                     /locus_tag="ACHKNP_RS16920"
                     /old_locus_tag="pgaptmp_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGU4705497.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15075..15953
                     /locus_tag="ACHKNP_RS16925"
                     /old_locus_tag="pgaptmp_003385"
     CDS             15075..15953
                     /locus_tag="ACHKNP_RS16925"
                     /old_locus_tag="pgaptmp_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGU4705498.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16159..17166
                     /locus_tag="ACHKNP_RS16930"
                     /old_locus_tag="pgaptmp_003386"
     CDS             16159..17166
                     /locus_tag="ACHKNP_RS16930"
                     /old_locus_tag="pgaptmp_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HGU4705499.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
BASE COUNT         4338 a         4561 c         4354 g         3958 t
ORIGIN      
        1 taaggttata aatgaataaa gttacaaaaa cagctattgt gggcttactt gcgcttttcg
       61 caggtaatgc cgctgcaacc gatggtgaaa tagtttttga tggcgagatt ttaaaatccg
      121 cttgtgaaat caatgactct gataagaaaa ttgaagttgc tcttggtcac tataatgccg
      181 aacagtttcg ttctgttggc gatcgtagcc caaaaattcc attcactatt cctttagtta
      241 actgcccagt gactgggtgg gagcacgata acggcaacgt tgaagcgtct ttccgtttat
      301 ggctggaaac gcgggataat ggcaccgtcc ctaacttccc taatctggca aaagtgggct
      361 cttttgctgg cacagcggca acaggtgtgg gtatccgtat tgacgatgcg gaaagcggaa
      421 accttatgcc actgaatgct atggggaatg ataacacggt ttatcagatc ccagcggact
      481 ccaatggtat tgtcaatgtt gacctcatcg cttactacgt atcaaccgtt gaagcatcag
      541 aaatcacccc aggggaagca gacgctgtcg ttaacgtaac gctggattac cgttaatacg
      601 ttacggcgtt atctgacctt tcagataacg cccttttcct tcctctttct cgttgtatca
      661 ggttgaaaaa taactatgtc aaaacgaaca ttcgcggtga taataacctt gttgtgtagc
      721 ttctgtatcg gccaggcgct tgcagggggt atcgttttac agcgaacgcg agtgatctat
      781 gatgccagcc gcaaagaggc tgcgttacct gtcgcaaaca aaggcgcaga aacgccttat
      841 ttactgcaat catgggtaga taatatagat ggtacaagcc gtgccccatt tattataacc
      901 ccaccgctat ttcgtcttga agccggcgat gactcatcac tgcgaattat taaaacagct
      961 gataacctgc ctgaaaataa agagtcgctg ttctacatta atgttcgtgc cattccagca
     1021 aagaaaaaat cagatgatgt taatgctaac gagttgacgc tggtatttaa aacacggatc
     1081 aaaatgtttt atcgccccgc acacctgaag ggacgggtaa acgatgcgtg gacatcactg
     1141 gaatttaaac gtagtgacca ttcactcaat atatataacc caactgaata ttacgtcgta
     1201 tttgccggac tggcagtcga taaaaccgat ctcacaagca aaattgaata tatcgcgccc
     1261 ggagaacata aacagttacc acttcctgca tctggcggaa agaacgtgaa atgggctgca
     1321 atcaatgatt atggcggcag ttccgggaca gaaactcgtc cactgcaata acaaatataa
     1381 aaaacacagg tcatcaggga atgccacaac gacaccacca gggacataaa cgcacaccga
     1441 aacagttggc gctcatcatc aaacgctgtt tgccgatggt gctcactggc agcggcatgc
     1501 tttgcactac cgctaacgcc gaagagtatt atttcgaccc cattatgctg gaaactacaa
     1561 aaagtggtat gcaaacaact gatctgtcac gtttttcaaa aaaatacgca caactaccag
     1621 gaacttatca ggttgatatc tggctgaata aaaagaaggt ttcacagaaa aaaattacat
     1681 ttaccgccaa tgcagagcaa cttctgcagc cacagtttac ggtagaacaa ctacgtgagc
     1741 tgggtattaa ggtggatgaa atcccggcgc tggctgaaaa agatgacgat agcgtgatca
     1801 actcgcttga acaaatcatt cccggtacag ctgctgaatt tgatttcaat catcagcgac
     1861 ttaatttgag cattccccaa attgcactgt accgtgatgc aagaggttac gtctcccctt
     1921 ctcgttggga cgatggtata ccaacgctgt ttaccaacta ctcgtttaca ggttctgata
     1981 accgttaccg ccagggcaat cgtagccaac gacagtacct gaatatgcaa aatggtgcta
     2041 attttggccc ctggcgatta cgtaactatt ctacgtggac acgcaacgat caggcgtcaa
     2101 gctggaacac tatcagtagt tatttacaac gtgatatcaa ggcgttgaag tctcagttgc
     2161 ttctgggaga aagcgccacc agcggcagta ttttttccag ctacaccttt actggcgtgc
     2221 aactcgcttc cgacgataat atgttgccaa acagccagcg cggatttgcc ccaacggtac
     2281 gcggtatcgc aaacagtagt gcaatcgtga ctatcaggca aaatggttat gtgatctatc
     2341 aaagcaacgt gccagcgggt gcctttgaaa ttaacgatct ctacccctct tccaacagcg
     2401 gcgatttaga agtcacgatt gaagaaagtg acggtacgca acgtcgcttt atccagcctt
     2461 attcttcatt acccatgatg cagcgacctg ggcatctaaa atatagcgcg accgctggac
     2521 gctatcgcgc tgatgcaaac agtgatagca aggaacccga atttgctgaa gccacggcaa
     2581 tatatggttt gaataatact tttacgctgt atggcggcct gctcggttct gaagattatt
     2641 atgcgctggg gatcggtatc ggcggcacac ttggcgcact gggcgcgttg tcgatggata
     2701 tcaacagagc tgacacccaa ttcgataacc ggcactcttt tcatggctat caatggcgta
     2761 cgcagtacat caaagatatc ccggaaacca acaccaatat cgctgttagc tactatcgct
     2821 ataccaacga tggctatttt agttttgatg aagccaatac ccgtaattgg gactataaca
     2881 gtcgccaaaa aagtgaaatt caattcaaca tcagccagac aatatttgat ggggtaagtc
     2941 tgtatgcctc cggttcgcag caagactatt ggggcaataa cgataaaaac aggaatatct
     3001 cagttggggt ttccggtcag caatggggaa ttggttacag cctgaattat caatacagtc
     3061 gctacacgga tcaaaataat gaccgcgcac tctctttgaa tctcagtatt ccgttagaac
     3121 gctggttacc gcgtagccgg gtttcctatc agatgaccag ccagaaagat cgcccaaccc
     3181 aacatgaaat gcgtcttgat ggctcactgc tggatgatgg tcgcctgagc tatagcctgg
     3241 aacaaaatct ggatgacgat aacaaccata acagtagcct gaacgccagt taccgttcac
     3301 cttatggcac cttcagtgcc ggatacagct acggtaatga cagcagccaa tacaattacg
     3361 gcgttaccgg cggcgtggtt atccatcctc atggcgtgac gctctcgcaa tatctgggca
     3421 acgcttttgc gcttatcgat gctaatgggg cttctggcgt gaggatacaa aactatccgg
     3481 ggattgctac tgatcccttt ggctatgcag tggttcctta tctcacgact taccaggaaa
     3541 accgtctctc ggtagatact acgcagctgc ccgataacgt cgatcttgag caaacaacac
     3601 agtttgtggt gcccaacaga ggtgcaatgg tagcggcgcg tttcaacgcc aatatcggtt
     3661 atcgcgtact tgttacagtc agcgatcgca acggtaaacc gttgcccttt ggcgctcttg
     3721 ccagcaacga tgagacgggg caacaaagta tcgtcgatga gggcggcata ctatatctct
     3781 ctgggatatc gagtaaatca caaagctgga ctgtacgctg gggaaatcag gcagatcaac
     3841 aatgtcagtt tgcttttagt acaccggatt cagaaccaac aacctctgta ttacaaggca
     3901 cggcgcagtg ccattaagga taaaaaaatg aaaagagcgc ctcttataac aggacttttg
     3961 ttgatatcca catcctgcgc ttatgcctcc tcaggagggt gtggagctga cagcactagc
     4021 ggtgcgacaa attacagcag tgtggttgat gatgttacgg tgaaccagac agataacgtg
     4081 acaggacggg agtttacctc tgccacgcta agtagcacta actggcaata cgcctgtacc
     4141 tgctctgcgg gtaaggcagt taaacttgtc tatatggtca gccctgtact taccaccact
     4201 ggacatcaag caggatatta caaactcaat gatagtctgg atattaaaac cacattacag
     4261 gcgaatgaca ttccaggact cgtgactgac cagaccgttt ctgttaacac ccgattcaca
     4321 cagataaaaa gcaacaccgt atattctgct gcaacccaaa cgggtgtttg ccagggtgac
     4381 acgtctcgtt atggacccgt taatattggt gcaaatacca cctttaccct gtatgtcacc
     4441 aagccatttc tcggctcgat gaccattccg aaaacggata ttgccgtcat taaaggcgca
     4501 tgggtcgatg gaatgggaag cccgtctaca ggtgacttcc atgatttagt caagttatcg
     4561 attcagggaa atctcaccgc cccacagtcg tgcaaaatta atcagggcga tgttattaag
     4621 gttaattttg gattcatcaa tggtcagaag tttaccaccc gcaatgccat gccagacggt
     4681 tttactccag tagactttga tatcacttat gactgtggtg atacttcaaa gattaaaaac
     4741 tcgttgcaaa tgcgcatcga cggtacaact ggggtagtag accagtacaa cctggtcgcc
     4801 agacgaagaa gttcagacaa tgcgcccgat gtcggtattc gtattgaaaa tctcggcggc
     4861 ggagttgcaa atattccttt tcagaacggt atccttcccg ttgatccttc cgggcatggc
     4921 accatcaaca tgcgcgcctg gccagttaat ctggtcggtg gtgagctgga aacaggaaaa
     4981 tttcagggca ccgccaccat taccgtcatc gtgcggtaaa agaagacgtt gcgttttgat
     5041 taacgcaacg cttcggcact cttacccctg ctgctccagc gcatacttat acagcgcatt
     5101 tttcttcacg ccgtgaattt ctgccgccag cgccgccgct tttttcaacg gcagttctgc
     5161 ctgtagcagc gccagcgtgc gcagggcatc ggcgggtaag tcttcttcct gtgctttatg
     5221 accttcgata atcagcacca tttcgccttt gcgacggttt tcatcttctt ttacccacgc
     5281 cagcagctcg ccaacgggcg cgccgtgaat ggtttcccag gttttggtca gctcacgcgc
     5341 cagaaccacg tagcgggatt cgcctaatac cgcaacgata tcttccaggc tatctaacag
     5401 acggtgggtg gattcataaa aaatcagcgt gcgcggctcc gcttcaatgg cttttagcgc
     5461 atcacggcgg ccttttgatt tggcaggtaa aaagccttcg taacagaaac ggtcagaggg
     5521 taaacccgcg gcgcttaacg cagtgatagc agcacacggc ccgggtagcg gcaccacgca
     5581 aatccccgct tcacggcagg tacgcaccag atggtagcca ggatcgttaa ttagcggcgt
     5641 tccggcatcg gaaaccagcg caatgttctg cccttcttgc agcttcgcca gcagcgtttc
     5701 ggctttttgt tgttcgttat ggtcgtgcag cgcaaacaac cgggcattaa tcccaaaatg
     5761 ttgcagcaat aaaccggtgt gacgagtatc ctcggcggca atcagatcaa cggcctgtaa
     5821 tacctctaac gcacgctggg tgatatccgc cagattgccg attggcgtcg gtacaatgta
     5881 aagctggccc tgagaattat ccgccgattg gtgttgtttc attgtgtcgt ccgtattgcc
     5941 gatttaatat tgagcattgc gtaaaaaaat atcactggat acattatggt accctcaaca
     6001 ttttctcgtt tgaaagccgc gcgttgtctg cctgttgttc tggcagccct gattttcgcc
     6061 ggttgtggca cccatactcc cgatcagtcc actgcttata tgcagggcac ggcgcaggct
     6121 gattctgcct tttatcttca gcagatgcag caaagctctg atgataccag gatcaactgg
     6181 caattactcg ccattcgtgc actggtgaaa gaaggtaaaa ccgggcaggc ggttgagttg
     6241 tttaaccaac taccgcaaga actgaacgat tctcagcgtc gcgagaaaac actgctggcg
     6301 gtagagatta aactggcgca gaaagatttt gctggcgcgc aaaacttgct ggcgaaaatc
     6361 acacctgccg atttagaaca aaaccagcaa gcgcgttact ggcaggcaaa aatcgatgcc
     6421 agccaggggc gtccttccat tgatttactg cgcgcgttaa ttgctcagga accgctgctc
     6481 ggcgcgaaag aaaaacagca gaatattgat gccacctggc aggcgctctc ctccatgact
     6541 caggaacagg cgaatacgct ggtgatcaac gccgacgaaa atattctgca aggctggctg
     6601 gatctgcagc gcgtctggtt tgataaccgt aacgatcccg acatgatgaa agccgggatc
     6661 gccgactggc aaaaacgtta tccgaacaat ccgggcgcga aaatgctgcc aacgcagttg
     6721 gttaacgtaa aagcgtttaa accagcctcg accaacaaaa tcgccctgct gttgccgctg
     6781 aatggccagg cagcggtatt tggtcgcact attcagcaag gctttgaagc ggcgaaaaat
     6841 atcggcactc agccagtggc ggctcaggta gctgccgcac ctgccgcaga cgtagctgaa
     6901 caacctcagc cgcaaaccgt ggatggcgtt gccagcccgg cacaagcctc ggttagcgat
     6961 ctgaccggtg aacagcctgc ggcacagcca gcgcctgtaa gcaccccggc agcaaccacc
     7021 acagcggtaa gcgcgccagc gaatccatcc gcagaactga aaatctacga tacctcatca
     7081 caaccactta gccagatctt aagccaggtt cagcaggatg gcgcgagtat tgtggttggt
     7141 ccgttgctga aaaataacgt tgaagagttg cttaagagca acaccccgct gaacgtgctg
     7201 gcactgaacc agccggagaa tatcgaaaac cgcgtcaata tttgttactt cgcgctttca
     7261 ccggaagacg aagcgcgcga tgcagcgcgt catattcgtg accagggtaa acaagcgccg
     7321 ctggtgctga tcccacgcag ttcattgggc gatcgcgtag ccaatgcgtt tgcgcaggag
     7381 tggcagaaac tgggcggcgg caccgttctg caacaaaaat ttggttccac cagcgaatta
     7441 cgcgcgggtg ttaacggcgg ttctggtatc gctttaacgg gtaccccgat tactcccaga
     7501 gcgacaaccg actccggcat gacgaccaac aatccaacgc tgcaaaccac gccaaccgat
     7561 gaccagttca ccaataatgg cggtcgtgtc gatgcggtgt acattgtggc aacgccgggt
     7621 gaaatcgctt ttatcaaacc gatgatcgcc atgcgtaacg gtagccagag cggtgcaacg
     7681 ctgtacgcca gctcccgcag tgcgcaaggg accgctggcc cggatttccg actggagatg
     7741 gaaggcttgc agtacagcga aatcccgatg ctggcaggcg gtaatctacc gttaatgcag
     7801 caggcactca gcgcggtgaa taacgattat tcactggctc gcatgtatgc gatgggcgtc
     7861 gatgcctggt cgctggcaaa tcatttctca caaatgcgcc aggttcaggg ttttgaaatc
     7921 aacggtaata ccggaagcct gacggcaaac ccggattgcg tgattaacag gaagttatca
     7981 tggctacagt accaacaagg tcaggtagtc cccgccagtt aaccaccaaa cagaccggcg
     8041 atgcgtggga agtacaagcg cgtcgctggc tggaaggcaa aggactgcga tttgtcgccg
     8101 ctaacgtgaa cgagcgtggc ggcgagatcg atctgataat gcgtgaaggc cggaccaccg
     8161 tttttgtcga ggtacgctat cgccgctctg cgctttatgg cggcgcggca gccagtgtga
     8221 cccgcagcaa acaacacaaa ttattacaga ctgcccgctt gtggctcgcg cgtcataatg
     8281 ggagttttga tactgtggat tgccggttcg atgtggtagc cttcaccggg aatgaggttg
     8341 agtggattaa ggatgccttt aatgaccact cataattaag gtttaaggat tagcgtgcaa
     8401 gaaagaatta aagcttgctt cactgaaagc attcaaactc aaattgcggc ggcagaggcg
     8461 cttccggatg ccatctcccg tgcagccatg acgctggttc agtctctgct caatggcaac
     8521 aaaatcctct gttgtggtaa tggaacttcc gctgccaatg cacagcattt tgctgccagc
     8581 atgatcaacc gtttcgaaac ggagcggccc agcttacctg ccattgcact aaatactgat
     8641 aatgttgtct taacggcgat tgccaacgat cgcttacatg atgaagtgta tgcaaaacag
     8701 gtgcgggcgc tgggtcatgc gggagatgta ttgttagcca tttccacccg tggcaacagc
     8761 cgcgatattg taaaagcggt agaagctgcc gttacgcgtg atatgactat tgtggcattg
     8821 accggctatg acggcggcga acttgcaggt ttgttagggc cacaagatgt ggagatccgc
     8881 attccttcgc atcgtagtgc tcgcattcag gaaatgcata tgctgacggt aaattgcctg
     8941 tgcgatctga tcgataacac gcttttccct caccaggatg tttaaggaga atacatgaag
     9001 gcattatcgc caatcgcagt ccttatttcc gcgctgctgt tgcaaggttg tgttgccgct
     9061 gccgtagtgg gtaccgctgc tgtgggtacc aaagccgcaa ctgacccacg cagtgtcggc
     9121 acccaggtgg acgatggtac cctggaagtg cgcgtgaaca gcgcattgtc gaaagacgaa
     9181 cagattaaga aagaagcccg tattaatgtc acggcgtatc agggcaaagt gctgctggtt
     9241 gggcagtcac caaacgctga actttcggct cgcgccaaac agattgctat gggcgtagac
     9301 ggtgccaacg aagtgtataa cgagattcgt cagggccagc cgattggtct gggcgaagcg
     9361 tctaacgata cgtggatcac caccaaagtg cgttcgcagc tcttaaccag cgacctggtg
     9421 aaatcgtcca acgtgaaagt gaccaccgaa aacggtgaag tgttcctgat ggggctggtg
     9481 actgaacgtg aagcgaaagc ggcggcagat attgccagcc gggtgagcgg cgtaaagcgg
     9541 gtaaccacag cgtttacgtt tattaaatag cagccccttg taatgcctga tgcgacgctt
     9601 gccgcgtctt atcaggccta caacgatgca aaccgtaggt cggataaggc gtttacgccg
     9661 catccgacat gttttccctc aaatcagaac aacagcgcca gcccgccgac aatcacacca
     9721 gacactgcca ccatcgcccc tgtcagccat aaggctttcg ctgggaacgc tttgcgcagc
     9781 ataatcagtg acggcaaact caccgccggg agcgtcatca acaatgccag cgccggagca
     9841 gttcccatac ctgccagcat catcgtttgt acaatcggaa tttctgctgc cgtgggaatg
     9901 acaaacaagc atcctgctac cgccatcgcc atcacccaca tcaggctgtt atcgacagca
     9961 ccatcggcat gggggaataa ccagacgcga gcggcaccca acaccagtac tgcaaggatg
    10021 taaaccggga tcgtactcca gaaaagcgtc catagcgccc tgccccagcg gctaaaaaaa
    10081 ccgccctgtg cttccggtat gtcaatttcg accggtgcct gcgtttgcgg tgtttcacgc
    10141 acccattttt gcaccagcgt cgcaatcagc aacaccatca ccagcccggc caccagacga
    10201 atcgccgcaa aaccccagcc gaggacaaac cccataaaca ccagcgtcgc cgggtttaac
    10261 accggattgc ccatccagaa tgccagcgca ccgcccatcg acacctgttg gcgacgcatt
    10321 cccgccgcga ccggagccgc acagcaggta cacatcatgc ccggcagcga aaacagcgtt
    10381 cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac gcaacaacca gtcacgcggg
    10441 atcagcacct gaatcaacga accgaggatc acccccagca ctgccgcttt ccataccgcg
    10501 aggaaataga gcatcgcgta atccaacgcc gcctgccacg gattagcatc cgcctgcgca
    10561 aggatagatt taccgatact gtgggtttcg gcagcagtaa acgctttgcc gtagtaaggt
    10621 tgccatttca cataccagag accggcaatg acaacgagaa agaaaagcgc gggtttccac
    10681 cactgaatgg gcgttgccgc ctgagatgaa gactgaccag tcatagcatt ccccaggaat
    10741 aagttatgat aattgagcgc gtgaatatta cgctcactat caattcttgg gaataattat
    10801 tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca caccttcatg cccgggccgc
    10861 atcgcctctg ccagcatgac ccgcgccacg tctctcgcat caatggattt ccagttaccg
    10921 ggtaataaac ggaacagcgg cgcaaaaagc gtttcgttca tccgctgttt gctacgatcg
    10981 cccagtaaca tcgacgggcg agcaatggtc aatttcggcc agttctgggc gattaatgct
    11041 tcttccatct cccctttgac gcggttatag aaaaacggcg agtgggcatt ggcacccata
    11101 gcactgacca ccagcatatg ctgcgcgccc agtcgccgcc cggttaatgc ggtatccact
    11161 accagcgtgt aatcggcatg aataaacgcc tctttgctcc ccgcttctcg ccgcgtggtg
    11221 ccgagacaac aaaacacaat gtcgatggga tcggtgacct gcgccagcgc gtcagtcagt
    11281 tgcggatcat ggggattaaa caccccgggc atatcgccca acggacgtcg cgtcggcgcg
    11341 gctatggcgt taactttcgg ttcgttaatc aacatccgca gcaggtgacc gcccaccagc
    11401 cccgttgcgc ctgtaatcag tacctgactc atcttcgctc ctttacagaa ttgtctgcct
    11461 tgcgctccac ggctcatgca ccaggcttaa tagaccatga ggtaattatc cccgattgtg
    11521 ggaaattcgc ctcatccaat gcaacaacgc ggaggaagca tgagtaagaa aattgccgtt
    11581 ttaatcactg atgaatttga agattcagaa tttacttcac ccgcagacga gttccgtaaa
    11641 gccggacacg aagtgattac cattgaaaaa caagcgggta aaacggtgaa aggcaaaaaa
    11701 ggagaagcca gcgtgaccat cgataaatcc atcgatgaag tgacgccagc ggagtttgat
    11761 gccctgctgc taccgggcgg ccattcaccg gattatctgc gtggcgacaa ccgttttgtc
    11821 acctttaccc gtgattttgt gaatagcggc aaacctgttt tcgccatctg tcacggtccg
    11881 cagctgctga tcagcgccga tgtgattcgg gggcgcaaac tgaccgcggt taaaccgatc
    11941 attattgatg ttaaaaacgc gggcgcggaa ttttacgatc aggaagtcgt ggtcgataaa
    12001 gatcagctgg ttaccagccg gacaccggac gatctgccag cgtttaaccg cgaggcgtta
    12061 cgcctgctcg gtgcctgagc cgcgtagcca aatcattttt ttaccaaagc ccagcgtgtt
    12121 gtcggtgaat ttgatttcat cgagccggat ttcccacacc ggggccgaca gcattctggc
    12181 aaccggaaag cgacgattgt acgccttgcg cgcgaggtcg ctttcctcac cttccagcct
    12241 gcggatctca cctttaaact gcacgccgcg aattaacgct accgttttcg gctgaccgtt
    12301 taccgttccg gcaaccgcag cctgcggccc actcatctgc gcgtggcgcg ttttttcttc
    12361 cgtcaaaatg tagaaggcga ctttctgcgc atcaaacaga taaaaagcat tagcgcacca
    12421 gagttcccct tcctgctgca cacaccaggt gacaacatgt tgttttgcca accaacggct
    12481 gatggtgatg agtgtttcca ttgctgttct cttttatact gtgggcctga ccttaacatg
    12541 cgacgcagac gatgacaccc tggtttcttt acttgatccg taccgccgac aataagcttt
    12601 ataccgggat caccacggat gtcgaacgcc gctatcagca gcaccaaagc ggcaaagggg
    12661 cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt ttctgcgcca gtgggcgatc
    12721 gttcgctggc gttacgggcg gaatatcgcg ttaagcaact gaccaaacgg caaaaagagc
    12781 gtctggtagc ggaaggcgca gggtttgcag agctgttaag cagtctgcaa accccggaga
    12841 ttaaaagcga ttgaaatgct cgtgatactc aaccagacca gttacgccat tcaacgcgtc
    12901 atccgctaaa cgatgtacct ggaaggcgct ttcagtgcct ggccagcggc aacgcaggtc
    12961 gtgatgtgcc gccagttcaa agccaaaacg gctgtacagc gccggatcgc ccagcgtcac
    13021 cactgcggca tagccgaact cattaagcga atcgagtcct tcatagacca gttggcgtgc
    13081 cagcccctgt ccacggtatt tttcatcaac cgccaacggt gccatgccga cccattgcag
    13141 gtcttcgccc tgcacatcaa ccggactaaa tgccacatag ccaatgacct gaccttcgtc
    13201 atctgtcgcc accagcccca gcgtcagaaa gccatcttca cgcagatcgt gaaccagctt
    13261 cgcttccgca tcactttcga atgagcgacg tagcagggca tcaatacccg gcgcatcaat
    13321 gggaatttct actcgaatta gcatggttca cctaccgatg tctgtttggt ttcaggcgcg
    13381 gttttcattc ccgcctcaac aaaatccgcc agttgcagca gcatcatgcg taaggctttc
    13441 ggcatttgct ccagttcaat ggcgtccatc aggtttttca catacagccc cagctccgta
    13501 tcgccttcaa tcaccagccg acgctggaag aagagcgtat ccggatcttg tttacgcgcc
    13561 gcaatcatca gcagatcgct ggcgtcggca ctaaaactca catcagcttg cgcgttctgg
    13621 ctgaccacca gtttgccatt caccaccgag gtaaaccatt gcaggtcaat atcacgcaca
    13681 tgaatactta accagcggcc ttcaagaaac tccagctcgc catcatccag cgcctgacgg
    13741 aattgccagc ttaagacctg ctcaagaacc tggcgtttta gcgcaaatgg cgtcagtttt
    13801 accggtacac tcaacagaga tggccccaaa tgcacaatac gggaacgcag tttatccaac
    13861 acgagtttta ctccctgttt caacaatcat cctattttgc catatcagaa aaataacata
    13921 gcggtataaa tcaacaattc catatgaaat tgctgctacc accaatacaa ctttaactgc
    13981 cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc cagttcgtta acatttttgc
    14041 gttttgatag cgcaaccttc aggaaaaatt atggagctgc tctgccctgc cggaaatctc
    14101 ccggcgctta aggcggccat cgaaaacggc gcagatgctg tttatatcgg gctaaaagat
    14161 gataccaatg cccgtcactt cgccggcctt aactttaccg agaaaaaatt gcaggaagcg
    14221 gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg cgattaacac ttttgcgcat
    14281 ccggacggtt acgcccgttg gcagcgtgcc gtggatatgg cggcgcagct gggtgccgac
    14341 gcgctgatcc tcgccgacct cgccatgcta gagtacgccg ccgagcgtta cccgcatatt
    14401 gagcgtcatg tgtcagtgca ggcttcggcg accaatgaag aggcgattaa cttttatcat
    14461 cgccattttg acgttgctcg cgtggtgctg ccgcgcgtgt tgtcgattca tcaggtgaaa
    14521 caactggcac gggtcacacc tgtaccactg gaagtctttg ctttcggcag cctgtgcatt
    14581 atgtcggaag gtcgttgcta tctgtcgtcg tatctgacgg gtgagtcgcc caacaccgta
    14641 ggcgcgtgtt ctccggcccg tttcgtgcgc tggcaacaaa cgccgcaggg gctggaatcc
    14701 cgcctgaacg aagtgctgat cgaccgttat caggacggcg aaaacgcagg ttatccgacg
    14761 ctatgtaaag ggcgttatct ggtggacggc gagcgctatc acgcgctgga agaaccaacc
    14821 agtctcaata ccctggaact gctgccggag ttaatggcgg cgaatattgc ttcggtgaaa
    14881 attgaaggcc gccagcgtag cccggcgtat gtcagccagg tggcgaaagt ctggcgtcag
    14941 gctatcgacc gttgtaaggc cgatccgcaa aacttcatac cgcaaagcgc gtggatggag
    15001 acgctcgggt cgatgtccga aggcacgcag accacccttg gcgcgtatca ccgtaaatgg
    15061 cagtgagaag agcaatgaaa tattccttag ggccagtgct gtggtactgg ccaaaagaga
    15121 cgctggaaga tttttatcag caggccgcca ccagcagcgc cgacgtgatt tatcttggtg
    15181 aagcggtatg cagcaagcgc cgggcaacca aagttggcga ctggctggag atggcaaaat
    15241 cgctcgccgg aagtggcaag cagattgtgc tctccacgct ggcgctggtg caggcatcct
    15301 ctgaactggg cgaactgaaa cgctatgttg agaacggtga gtttctgatt gaagccagcg
    15361 atctcggcgt ggtgaatatg tgcgctgaac gcaaactgcc gttcgtcgcc gggcacgcgc
    15421 tgaactgcta caacgcggtg acgctgaaaa tattgctcaa acagggcatg atgcgctggt
    15481 gtatgccggt ggagctttcc cgcgactggc tggtgaatct gcttaatcag tgcgatgagc
    15541 tgggcattcg caaccagttt gaagtggaag tcctgagcta cggtcatcta ccgctggcct
    15601 actccgcccg ctgctttacc gcgcgttcgg aagaccgccc gaaagatgag tgtgaaacct
    15661 gctgcattaa gtatccgaac gggcgcaacg tgctgtcgca ggaaaaccaa caagtgtttg
    15721 tactcaatgg cattcagacc atgagcggct acgtttacaa cctcggtaac gagctggcat
    15781 ccatgcaggg gctggttgat gtggtccgcc tgtcaccgca gggtactgac actttcgcga
    15841 tgctcgacgc cttccgcgct aatgaaaatg gcgctgcgcc actaccgttg acggcaaaca
    15901 gcgactgtaa cggctactgg cggcggctgg ccgggctgga gctgcaagcc taagtaaata
    15961 tgctcgtcat acttcaagtt gcatgtgctg catctgcatt cgttcacccc agtcacttac
    16021 ttatgtaagc tcctggggat tcactctctt gtcgccttcc tgcaactcga attatttaga
    16081 gcacagctca ctttgttaac aactttaact actctttaat gcagtattaa agattaatcg
    16141 gtaacaaagt gagctgttat gactgataaa accattgcgt tttcgctact cgatctggcc
    16201 cccattcccg aaggttcttc agcgcgagaa gcattctccc actctctcga tctcgcccgt
    16261 ctggctgaaa agcgcggcta tcatcgctac tggctggcag aacaccacaa tatgactggc
    16321 attgccagtg ctgccacgtc ggtattgatt ggctatctgg cggcgaatac caccacgctg
    16381 catctggggt ctggcggcgt gatgttgcct aaccactcac cgttggtcat tgccgaacag
    16441 ttcggcacgc tcaatacact ctatccgggg cgaatcgatt tggggctggg tcgtgcgcca
    16501 ggtagtgacc aacggacaat gatggcgctg cgtcgccata tgagcggcga tattgataat
    16561 ttcccccgcg atgtcgcgga gctggtggac tggtttgacg cccgcgatcc caatccgcat
    16621 gtgcgcccgg taccaggcta tggcgagaaa atccccgtgt ggttgttagg ctccagcctt
    16681 tacagcgcgc aactggcggc gcagcttggt ctgccgttcg cgtttgcctc acacttcgcg
    16741 ccggatatgt tgttccaggc gctgcatctt tatcgcagca acttcaaacc gtcggcacga
    16801 ctggaaaaac catacgcgat ggtgtgcatc aatattatcg ccgccgatag caaccgcgac
    16861 gctgagtttc tgtttacctc aatgcagcaa gcctttgtga agttgcgccg tggcgaaacc
    16921 gggcaactgc cgccaccgat tcaaaatatg gatcagttct ggtcgccgtc cgaacagtat
    16981 ggtgtgcagc aggcgctgag tatgtcgttg gtaggtgata aagcgaaagt gcgtcatggc
    17041 ttgcagtcga tcctgcgcga aaccgacgcc gatgagatta tggtcaacgg gcagattttc
    17101 gaccaccagg cgcggctgca ttcgtttgag ctggcgatgg atgttaagga agagttgttg
    17161 ggatagtgtg tcttaacgcg ggaagcctta tccgagctgg caacgctgtc c
//
LOCUS       DAZFWL020000095        17078 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFWL020000095 DAZFWL020000000
VERSION     DAZFWL020000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.095"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            75..1214
                     /gene="arnB"
                     /locus_tag="ACHKNP_RS16935"
                     /old_locus_tag="pgaptmp_003387"
     CDS             75..1214
                     /gene="arnB"
                     /locus_tag="ACHKNP_RS16935"
                     /old_locus_tag="pgaptmp_003387"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HGU4705500.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTPMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTTIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1218..2186
                     /gene="arnC"
                     /locus_tag="ACHKNP_RS16940"
                     /old_locus_tag="pgaptmp_003388"
     CDS             1218..2186
                     /gene="arnC"
                     /locus_tag="ACHKNP_RS16940"
                     /old_locus_tag="pgaptmp_003388"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HGU4705501.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2186..4168
                     /gene="arnA"
                     /locus_tag="ACHKNP_RS16945"
                     /old_locus_tag="pgaptmp_003389"
     CDS             2186..4168
                     /gene="arnA"
                     /locus_tag="ACHKNP_RS16945"
                     /old_locus_tag="pgaptmp_003389"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HGU4705502.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4165..5055
                     /gene="arnD"
                     /locus_tag="ACHKNP_RS16950"
                     /old_locus_tag="pgaptmp_003390"
     CDS             4165..5055
                     /gene="arnD"
                     /locus_tag="ACHKNP_RS16950"
                     /old_locus_tag="pgaptmp_003390"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HGU4705503.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5055..6707
                     /gene="arnT"
                     /locus_tag="ACHKNP_RS16955"
                     /old_locus_tag="pgaptmp_003391"
     CDS             5055..6707
                     /gene="arnT"
                     /locus_tag="ACHKNP_RS16955"
                     /old_locus_tag="pgaptmp_003391"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HGU4705504.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6704..7039
                     /gene="arnE"
                     /locus_tag="ACHKNP_RS16960"
                     /old_locus_tag="pgaptmp_003392"
     CDS             6704..7039
                     /gene="arnE"
                     /locus_tag="ACHKNP_RS16960"
                     /old_locus_tag="pgaptmp_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HGU4705505.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7039..7425
                     /gene="arnF"
                     /locus_tag="ACHKNP_RS16965"
                     /old_locus_tag="pgaptmp_003393"
     CDS             7039..7425
                     /gene="arnF"
                     /locus_tag="ACHKNP_RS16965"
                     /old_locus_tag="pgaptmp_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HGU4705506.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7419..7685)
                     /gene="pmrD"
                     /locus_tag="ACHKNP_RS16970"
                     /old_locus_tag="pgaptmp_003394"
     CDS             complement(7419..7685)
                     /gene="pmrD"
                     /locus_tag="ACHKNP_RS16970"
                     /old_locus_tag="pgaptmp_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HGU4705507.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7795..9150)
                     /gene="menE"
                     /locus_tag="ACHKNP_RS16975"
                     /old_locus_tag="pgaptmp_003395"
     CDS             complement(7795..9150)
                     /gene="menE"
                     /locus_tag="ACHKNP_RS16975"
                     /old_locus_tag="pgaptmp_003395"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HGU4705508.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9147..10109)
                     /gene="menC"
                     /locus_tag="ACHKNP_RS16980"
                     /old_locus_tag="pgaptmp_003396"
     CDS             complement(9147..10109)
                     /gene="menC"
                     /locus_tag="ACHKNP_RS16980"
                     /old_locus_tag="pgaptmp_003396"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HGU4705509.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10109..10966)
                     /gene="menB"
                     /locus_tag="ACHKNP_RS16985"
                     /old_locus_tag="pgaptmp_003397"
     CDS             complement(10109..10966)
                     /gene="menB"
                     /locus_tag="ACHKNP_RS16985"
                     /old_locus_tag="pgaptmp_003397"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HGU4705510.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10981..11739)
                     /gene="menH"
                     /locus_tag="ACHKNP_RS16990"
                     /old_locus_tag="pgaptmp_003398"
     CDS             complement(10981..11739)
                     /gene="menH"
                     /locus_tag="ACHKNP_RS16990"
                     /old_locus_tag="pgaptmp_003398"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HGU4705511.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11736..13406)
                     /gene="menD"
                     /locus_tag="ACHKNP_RS16995"
                     /old_locus_tag="pgaptmp_003399"
     CDS             complement(11736..13406)
                     /gene="menD"
                     /locus_tag="ACHKNP_RS16995"
                     /old_locus_tag="pgaptmp_003399"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HGU4705512.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13495..14790)
                     /gene="menF"
                     /locus_tag="ACHKNP_RS17000"
                     /old_locus_tag="pgaptmp_003400"
     CDS             complement(13495..14790)
                     /gene="menF"
                     /locus_tag="ACHKNP_RS17000"
                     /old_locus_tag="pgaptmp_003400"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HGU4705513.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14869..15174)
                     /gene="elaB"
                     /locus_tag="ACHKNP_RS17005"
                     /old_locus_tag="pgaptmp_003401"
     CDS             complement(14869..15174)
                     /gene="elaB"
                     /locus_tag="ACHKNP_RS17005"
                     /old_locus_tag="pgaptmp_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HGU4705514.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15229..15690)
                     /locus_tag="ACHKNP_RS17010"
                     /old_locus_tag="pgaptmp_003402"
     CDS             complement(15229..15690)
                     /locus_tag="ACHKNP_RS17010"
                     /old_locus_tag="pgaptmp_003402"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4705515.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15755..16672
                     /gene="rbn"
                     /locus_tag="ACHKNP_RS17015"
                     /old_locus_tag="pgaptmp_003403"
     CDS             15755..16672
                     /gene="rbn"
                     /locus_tag="ACHKNP_RS17015"
                     /old_locus_tag="pgaptmp_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HGU4705516.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16860..>17078
                     /locus_tag="ACHKNP_RS17020"
                     /old_locus_tag="pgaptmp_003404"
     CDS             16860..>17078
                     /locus_tag="ACHKNP_RS17020"
                     /old_locus_tag="pgaptmp_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HGU4705517.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVR"
BASE COUNT         3969 a         4618 c         4355 g         4136 t
ORIGIN      
        1 aaatggcaaa atatacacaa cactctttat agcaaatata agtggacagg tattcaatgg
       61 cggaaggaaa agcaatgtca gaatttttgc ctttttcgcg accagcaatg ggcgtggagg
      121 aactcgctgc agttaaagag gttctcgaat ccggttggat cacaaccggt ccgaagaatc
      181 aggcgcttga gcaagccttt tgccagttga cgggaaatca gcatgccatc gcggtcagtt
      241 cagccaccgc cggaatgcat atcacgctaa tggcgttgga aattggcaag ggcgatgaag
      301 tgattacgcc ttccctgacc tgggtttcaa ccctcaatat gatttccttg ttgggtgcaa
      361 cgccggtaat ggtggatgtc gaccgcgata cgccgatggt cacgcctgaa gcgatcgagt
      421 cagccataac gccacgcact aaagccatca ttccggtgca ttatgccggt gcgccagcag
      481 atattgacgc cattcgcgcc attggcgaac gttacggcat cgcagttatc gaagatgctg
      541 cccatgccgt cggtacgtat tacaaagggc gacatattgg cgcaaaaggt accactattt
      601 tttcatttca tgccattaaa aatattacct gtgcggaagg tggcctgatt gtaactgata
      661 atgaaaacct tgcccgccag ctacggatgc tgaaatttca cggtctgggt gtcgatgcct
      721 atgacagaca aacctggggc cgtgcaccgc aggctgaagt cttaacaccg ggctataagt
      781 acaatctgac cgatattaac gccgcaattg ccctgacaca gttagcaaaa ttagagcacc
      841 tcaacacccg tcggcgcgaa attgcccagc aatatcagca agcactggca gctctcccct
      901 ttcagccatt aagccttccc gcctggccgc acgttcacgc ctggcatctg tttattattc
      961 gtgtcgatga acaacgttgt ggtatcagtc gcgatgcgtt gatggaagcg ttaaaagaaa
     1021 gaggcattgg taccgggtta catttccgcg ccgctcacac acaaaaatat tatcgcgagc
     1081 gttttcccac gctgtcgtta ccgaataccg aatggaatag cgaacgcatc tgttctttgc
     1141 cgctgttccc ggatatgact accgccgatg ccgaccgtgt catcacagcc cttcagcaac
     1201 tcgcaggaca ataagccatg tttgaaatcc accctgttaa gaaagtctcg gtggttattc
     1261 ccgtttataa cgagcaggaa agcttaccgg aattaatcag gcgcaccacc acagcctgtg
     1321 aatcgttggg gaaagagtat gagatcctgc tgattgatga cggcagtagc gataattccg
     1381 cgcatatgct ggtcgaagcc tcacaagcgg agaacagcca tattgtgtct attttgctta
     1441 accgcaatta cgggcaacat tcagcgatta tggcgggatt cagtcacgtt accggcgact
     1501 taattattac ccttgatgcc gatctccaga atccgccaga agaaatcccc cgcctggtgg
     1561 caaaagccga tgaaggttac gacgtggtag ggactgtacg ccagaaccgc caggacagct
     1621 ggtttcgtaa aacagcttcg aagatgatta accggcttat tcagcgcacc accggcaaag
     1681 cgatgggtga ctacggttgt atgctgcgcg cctatcgccg tcatattgtc gatgcgatgt
     1741 tgcactgcca tgaacgtagc acctttatcc cgattctggc gaatatcttc gcccgccgtg
     1801 ccattgaaat tccagtacat cattccgagc gtgagtttgg tgaatccaaa tacagtttta
     1861 tgcgcctgat taatttgatg tacgacctgg tgacctgcct taccacaacg ccgctacgta
     1921 tgctgagtct gctcggcagc attattgcga ttggaggttt tagcattgcg gtgctgctgg
     1981 tgattttacg cctgaccttc ggaccacaat gggcggcaga aggcgtcttt atgctatttg
     2041 ccgtgctgtt tacttttatt ggcgctcagt ttatcggcat gggattactc ggtgaatata
     2101 tcggcaggat ctacaccgat gtccgcgccc gcccccgcta ttttgttcag caagttatcc
     2161 gtccatccag caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc acgatatggg
     2221 atgcctcggt attgaagctc tgctggctgc cggttacgaa attagcgcca tttttaccca
     2281 taccgataat cccggtgaaa aagcctttta tggttcggtg gctcgtctgg cagcggaaag
     2341 aggcattccg gtttatgcgc cggatgacgt taatcatccg ctgtgggtgg aacgcattgc
     2401 ccaactgtcg ccagatgtga ttttctcttt ttattatcgc catcttattt gcgacgaaat
     2461 tttgcagctc gctcccgcag gtgcatttaa tctgcatggt tcgctgttac caaaatatcg
     2521 tggtcgcgcg ccgctgaact gggtgctggt caacggtgaa acggaaactg gcgttacatt
     2581 gcaccgaatg gtgaaacgtg ccgatgccgg ggccattgtg gcgcaactgc gcattgccat
     2641 tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgccg cgcgccagct
     2701 actggaacag acattacccg ccattaaaca cggtaatatt ctggaaatcg cccagcgcga
     2761 aaacgaagcc acctgttttg gtcgcagaac gccggatgac agtttccttg aatggcataa
     2821 accggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc cgggtgcctt
     2881 cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag
     2941 caaagcacag ccggggagcg tgatttctgt tgcgccactg ctgattgcct gtggcgatgg
     3001 cgcgctggaa atcgtcaccg gacaggcggg cgacggcatt actatgcagg gctcgcaatt
     3061 agcgcagacg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg cctgcaccgc
     3121 ccgacgccgt acccgggtac tcatcctcgg ggtgaatggc tttattggca accatctgac
     3181 agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg gcagtgatgc
     3241 gataagccgt tttctgaatc atccgcattt tcactttgtt gaaggcgata tcagtattca
     3301 ttccgaatgg attgagtatc acgtcaaaaa atgtgatgtc gtcttgccgc tggtggcgat
     3361 agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg attttgaaga
     3421 gaatctgcgc attatccgct actgcgtgaa gtaccgtaag cgaatcatct tcccgtcaac
     3481 ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggaccatt ctaatttaat
     3541 cgtcggcccg gtgaataaac cacgctggat ttattcggta tcaaaacaat tacttgatcg
     3601 ggtgatctgg gcctatggcg aaaaagaggg tttacagttc accctcttcc gcccgtttaa
     3661 ctggatggga ccgcgactgg ataaccttaa tgcggcacga atcggcagct cccgcgctat
     3721 tacgcaactc attctcaatc tggtagaagg ttcaccgatt aagctgattg atggcggaaa
     3781 acaaaaacgc tgctttactg atattcgcga tggtatcgag gcgttatacc gcattatcga
     3841 aaatgcggga aatcgctgcg acggtgaaat tatcaacatt ggcaatcctg agaacgaagc
     3901 gagcattgag gaactgggcg agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca
     3961 tcatttccca ccgtttgcgg gctttcgcgt tgtcgaaagt agcagctact acggcaaagg
     4021 atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccaccgct gcctggactg
     4081 ggagccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct tcctgcgcac
     4141 cgttgatctt acggataaac catcatgacc aaagtaggct tacgcattga tgtcgatacc
     4201 tttcgtggca cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa gcataatatt
     4261 caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct ctggcgactg
     4321 gtgaagccac agtttttgtg gaagatgctg cgctcaaatg cggcatcgct ttatggctgg
     4381 gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc caatgccgat
     4441 atcattcgtg aagcggctaa acatcacgaa gtcggcctgc acgcctggga tcaccatgcc
     4501 tggcaagccc gtagcggtaa ctgggatcgg caaacaatga tcgacgatat tgcacgcggt
     4561 cttcgcactc tggaagagat tatcggtcaa ccggtaacct gttctgccgc tgcgggctgg
     4621 cgtgccgacc agaaggtgat cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat
     4681 tgtcgtgggg ccatgccgtt ccgtccattg ctcgaatcag gaaaccctgg cactgcgcaa
     4741 attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa agcagaagat
     4801 tttaacggtt ggttactcaa ccgcatcctg cgagataaag gcacgccggt ttataccatt
     4861 catgcagaag ttgaaggctg cgcttatcag cataattttg tggatctcct caaacgcgca
     4921 gctcaggaag gagtgacatt ttgcccttta agcgaactgt tatcagagac gttgccgctc
     4981 ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg ccaacaaatt
     5041 gcgggtagtc gctgatgaaa tcggtacgtt accttatcgg cctcttcgcg tttattgcct
     5101 gctattacct gttaccgatc agcacgcgtc tgctctggca acccgatgaa acgcgttatg
     5161 cggaaatcag tcgagaaatg ctggcatccg gcgactggat tgtgccccat ctgttagggc
     5221 tacgttattt cgaaaaaccc attgccggat actggattaa cagcattggg caatggctat
     5281 ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg
     5341 cgctggtgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct ctactcgcca
     5401 cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc gtgctcgatc
     5461 cgtttattgc attctggctg gtggcgggaa tgtgcagctt ctggctggca atgcaggcac
     5521 agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc ggcatggggg
     5581 tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg ccatgggtag
     5641 cgacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt atcagttgcg
     5701 tactgacggt tctcccttgg ggactggcga tagcgcagcg ggagcctgac ttctggcact
     5761 attttttctg ggttgagcat attcaacgct ttgcactgga tgatgcccaa catagagctc
     5821 cgttctggta ctacgtgccg gtcatcattg ccggtagcct gccgtggctg ggattactcc
     5881 ccggtgcact gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc tatttgttga
     5941 gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggtaaattg cccacctata
     6001 ttctttcctg ctttgcatct ctggcaatgc tgatggcgca ttacgctttg ctggcagcaa
     6061 aaaataatcc tctggcgctg cggattaatg gctggattaa catcgctttt ggcgtcactg
     6121 gcattattgc cacatttgtg gtctccccgt ggggaccaat gaacacgccg gtgtggcaaa
     6181 ccttcgagag ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg gcatttttcg
     6241 gctggtacac cttaacaaac gtcgaaaaga cctggccttt tgccgcgctt tgcccgctgg
     6301 ggctggcgtt gctggtagga ttttcaattc ctgacagagt tatggaagga aaacatccgc
     6361 aattttttgt cgagatgaca caagaatcac ttcagccaag ccgctatatt cttactgata
     6421 gcgtcggtgt tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc atcatgtatc
     6481 gccagacagg tgagttgaaa tacggcctta attatccgga tgcgaaaggg agatttgtca
     6541 gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt attactctcg
     6601 tgctttcggt tgaccgcgat gaagatatca acagtctcgc cattccgccc gcagatgcca
     6661 tcgatcgtca ggagcgtctg gtgctgattc agtatcgtcc caaatgatct ggctaacatt
     6721 agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg caacctgctt
     6781 tgtggcgata aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg
     6841 tcttggtctt gccatggtgc tctggctgct ggtcttgcag aacgtaccgg taggcattgc
     6901 ctacccgatg ttaagtctga attttgtctg ggtgacgctg gctgcagtaa aactgtggca
     6961 cgaaccggta tcgccgcgtc actggtgtgg ggtggcgttc attattggtg gcattgtgat
     7021 cctcgggagt acggtgtaat gggcctgata tggggattat tcagcgtcat tattgcctca
     7081 gttgcgcaat taagcctggg ttttgcggca agtcatctgc cgccgatgac gcacttatgg
     7141 gattttatcg cggctctgtt agcttttggc ctggatgccc ggatactgtt actcgggctg
     7201 ctgggttatc tgctttccgt tttttgttgg tataaaacgc tacataaact tgcgctaagc
     7261 aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc aatggtactt
     7321 cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg tattatgagc
     7381 gggttgatgc tgattttcct gcccacgaca aaacaacgtt actgagtttt ccctgccgct
     7441 gtacactgcc gttcccactc atcggggcta taactgcttg ccgagaggac tttcaccgta
     7501 tgcagctttt cacgattaat acaataaagc gcattctgca aaggcgagag taaatccccc
     7561 actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg
     7621 cagagcataa gtacatgtct gttatcttgt ttattgcagc acgatttttt gaccagccat
     7681 tccattgcat tatcctgttt gctaagagtt ttcacatcaa tttacagatt aacagataaa
     7741 ctctttatca acggcaatgg aatggcagaa atgagaagtg aaacggaggg ttaattattg
     7801 ctgacgttgc acccactctt ttagcgcctg acgtgaaatt ttaataccgc cgtttttcag
     7861 ctccggcggt aaagtcagcc agcgcaccgg ttgttgaaaa cgagccagct tatctttcac
     7921 ccattcgcca agatcaacgg tttgctggtc atactccacc accgctaccg gccgatgacc
     7981 aaactccttg tcggcaacgg gaacgataaa cacctgcaaa accgcaggat gtgcagcaat
     8041 aacacgctcg acttcttccg gctgaatacc ctctccgcca ctgaagaata gattgtctaa
     8101 acgtccgaca atggtcagct tgccattatg catctcaccg cgatcgcgcg tagcgtacca
     8161 gccttcgtca ttaaccagtg aaaccagttg cccgttacgc cagtaacctt ctgccatact
     8221 ggcagcccgc agccacactt cattattaac gattttcact tcccgacccg gcagcggcga
     8281 accaacgtct gccaggccgt cggcttcttt cgcacacacc gtggaggcaa actcggtcag
     8341 accatagccg caaaagcaac gaatcccctg ctcgcgcgcc tgttccgtca actcgaccgg
     8401 gatagccgcg ccgccaagta acaccgcttt cagggaaacg gaactgcggt taaccagcaa
     8461 acgccagagt tgtgttggca ccagtgaagc atgagtacag cctgccagca tttgctccaa
     8521 tggctgttta tcacgcaccg tcatccgcgc accagcgtat aaccagcgcc acataattcc
     8581 ctgaccggag acgtgaaaca gcggtaaaga gagcaaccag tcatcgtgat cgccaaacgg
     8641 aatcaacgat aacacccctt cggcactggc aagatgggcc tgataagtat ggacagcggc
     8701 tttcggcaac ccggtagaac ccgaggtcaa cgtcattgag cacagacgcg tcggctgcca
     8761 cgtagcggca tgtgcgcctt caacccgctg aatgtgcagc gacgttaacg ccggaaacgt
     8821 gttttcccca tccggcacca gcgcaaattg cagcgtcaga ttgggcagca attcttcaag
     8881 caacggttgc ggcagctgag ggttcacggg caacacccgc gccccgcatt gcagtaacgc
     8941 cagccaggcg agcagcgttt gcggcgtatt ccacgcccgc aacatcacgc cgctgccctc
     9001 aacaacgccc tgcgccgcaa agcctgaggc taattcatcg acgcgagcac aaagctcgcg
     9061 ccagttaagt tgctcgtcat taagacgtaa ggcgatggct tctccccgca cttgccgcca
     9121 gtgacgccac ggccagtcag agaagatcat aacaaccgct ccagtgcatc aacttccacg
     9181 acaggcagcg tgctacccgg ccagcgacgt acctgctgcg cctgcatcag atccagcgtg
     9241 tccagccctg gaatggtgtc cggcgttaac caggcggcaa tccgcgccag ttgcgttaag
     9301 cctaagctcg attcaatgga agaactgatc accgccgtca gccccagcgc gtgcgccgcc
     9361 tgtacctgct cgcgtacttt ctccagactg cccgtgagcg tgggtttgat aactaccgcg
     9421 cgcacgccct cttcagccac aaaggcaaaa tccggctcgc gcaagctttc atcccaggca
     9481 atggcaatgc cggtttcacg ggcaaacgct cgcgaatcat cgcgggtttt gcacggctct
     9541 tcgagaaacg cgatgcggtg gcgataatcc gggttaacat atttggcaaa ctgctggcct
     9601 ttcagcggtg tccaggcgcg atttgcgtca agacgcaaat gcagatccgg aattgcctcc
     9661 agcaacagat taaccaccat gccgtcgcgc acggcttcgt acaatcccac tttgaccttc
     9721 gccactttct cgcctggcat atcagcaagt ttgaggatca gatcgtcagg atcgccatta
     9781 cacagtggtg ccgcacggta attggccgcc tgcgggagcg tatctgccag ctccgccaat
     9841 gcacagctta cgccaaaggc aacggaaggc atctgcggta gctcgcaatc gcctgccagc
     9901 cagttattta cccaggcaag cagcacgctt tgcgcctctt cccaggtttc ctggctgaag
     9961 cccggcagtg gggagatctc cccccaccct tcgcgctcgc cctcacgcag gcaaacgtac
    10021 agcccgtcgc gggtttttaa ccgcctgtcg cgcagaacca cccccgcgtc catggggatc
    10081 tgccagcggt atacctgcgc gctacgcatt acggattccg tttaaatttg ctgaaatcag
    10141 gctgacgttt ctggttgaaa gcgttgcgac cttcctgacc ttcttccgtc atgtagaaca
    10201 gcatggtggc gttgcccgcc agctcctgca gccccgcctg cccgtcacag tcggcgttca
    10261 gcgcagcttt caggcagcgc agcgccatcg ggctgttttg cagcatttcg cggcaccaac
    10321 ggacggtttc tttttccaga tccgccagcg gtaccacggt gttgacaagg cccatatcca
    10381 gcgcctgttt tgcgtcgtac tgacggcaca ggaaccagat ttctcgcgct tttttctgcc
    10441 cgacgatacg agccatgtag gaagcgcccc agccgccgtc gaaggaaccg actttcgggc
    10501 cagtctgacc gaagatggca ttatctgctg cgatagtcag gtcgcacatc atgtgcagaa
    10561 cgtgaccacc gccgatggag tagccagcca ccatcgcgac aaccggtttc ggacaggtac
    10621 ggatctgacg ctggaagtcc agcacgttca agtgatgtac gccggaatca tctttatagc
    10681 cgccgtaatc accacgcact ttctggtcac caccggagca gaacgcttta tcgcctgcgc
    10741 cagtcagaat gatcacgccg atattgtcgt cataacgcgc atctgccagt gcctggatca
    10801 tctcttttac cgtcagagga cggaaggcat tgcgcacctg cggacgatta atggtgattt
    10861 ttgcgatacc gtcggtggat ttttcataac gaatgtcctc gaaaccttcg gagcagtcgt
    10921 gccattcaac cggtgcgtaa agcattgctt catcaggata aatcatattg tgtcctttag
    10981 tcagaaacgc aagatctgcg ccagacttgc gattacgcca gcgggatttt cccgatgcgc
    11041 gttatgtccg gcgcgaggaa tgacatggca gtcggcagcc agttccgccg ccagggcgcg
    11101 gaatttgctg tcacgttcac cacataaata ataaaacgca aatgtgcggg cgctaaggtt
    11161 agcacgtaaa tcaggctgga cggcgagaga agtcgcctcc agcatggcgg caagggttgc
    11221 gccattattg ttgctgcgca gcgccaccag ctcccggcgt tgatcgtcat tgagcgaggc
    11281 aaaaacaggc tgttgatacc agtcggcaaa taccgccgtt aacggttctg tgcaaaaacg
    11341 ctgcgcccat tggcgatcgg aacgctgacg ttccgcacgt tgttcagcat tttgcagccc
    11401 cggatgcccg ccttcgacaa taaccccaca aagccccgcc agcccctggc aagccgccat
    11461 catcgccacc cgtccgccaa gcgagtaccc caccagccag aagtcaagga tgttgtaact
    11521 aaccaaggtt ttacgcagta agtcggtgac atcatcaaat ccatcgacgc taatcgccgc
    11581 cgaaccacca tgacctggga gatcaacata caaccgtgag tagtcggcaa acgcctcgcc
    11641 cacttcttgc cattcgtggc aatcgccgga aaaaccgtgc aaaaacacca gccagggtaa
    11701 acctggtttt ccgtgttttg cctgcgcgtg caggatcata aatggcttac ctgcgccaga
    11761 agttgctgga gcgtttgcgc accatcggtg tcgttaacca ccatttcaat caccgtggtg
    11821 gttggcgtgc gccaggcgtc ggcaaatgcc gtttcaagtt cctgccagtt ttgcggacga
    11881 tgatatttca gctcgaacat cgcggcggcg tgctcaaaat ggacgttttg cggcatcaga
    11941 tagaaacgct cacgctcgct ttgcggcgtt ggcaacagcg agaaaatttg cccgccgttg
    12001 ttgttcacca caattaatac cagcggcgca gaaacctgac gcaataacgc cagcgcgttg
    12061 agatcgtaaa gtgcggagag atcgcccaca atcgccagcg tcggtttgcc gcttgcccgc
    12121 tgaacgcctg cggcggtcga aagcagtccg tcgataccgc tggcccctcg gttgctgtac
    12181 accgggtaac ctgccggaag ttgcgaaagc gcatcaatca gacgtaccac caggctgtta
    12241 ccaacaaaca attgcccctg ttcaggcaga tagtcgctga tgcgatgcgc cagttgcgct
    12301 tcgccaaacg catcacggcg ggcaataacc gcctgcattg cctgttccgc caggcgcggg
    12361 atttcaacgc accagggctg gcgtttttct gccggatgca gctccagcca gtcggcaata
    12421 ttggcaatta agcgacgtcc gcgatggtgt gccggatcaa gtcgcccttc aatgtcatca
    12481 acaatccagt actcttcggg ttcacagctt gcctgccatt gcaggagccg tttccccgtc
    12541 aggctgcttc ccagttgcac cacaatttgc gcctgctgca gctcgctggt cgctttggca
    12601 ttgcctaacc aaagatcggc acacggcagc ggctgcccgg tttgtgacag cacatcgcca
    12661 atcagcggcc agccaagagt ttgcgcccac agggcaactt ttttgccctc ttccgcactc
    12721 atgcgcccgg caaccaccac gccgcgcttt tgtcgccaga agaaccagtc gcgctgtttt
    12781 tcactttcca gacgaggcgc ttcacgcagc cacggtttgt cgtcctgcca ccagtcaccc
    12841 agacgctgtt gccagctaag cccggtatcg tccatttcgc catacagcgg ttcagcaaac
    12901 gggcagttga tatggactcc ccccgcatga agcgtaccga gagcgtggtc gatggtagaa
    12961 accagccaac gtgcggggat atcctgggtc gggcgcggca atgaaatact gtgcgtgggg
    13021 tgagaggcga acattcccgg ctggcgaatt gcctgattcg cgccgcagtc aattagctcc
    13081 ggcgggcgat cggcggttaa gagaatcagt ttttctccgg ttaacccggc ttcaatcagt
    13141 gccggataga gatttgccac cgccgtgccg gaggtcacaa tcaccgccac cggctgcttg
    13201 ctgactttcg ccagccccag cgccagatgc cccagtccac gctcatcgaa atgggtgtgg
    13261 tgaatgaatg cggaattctc cgccgccgct aacgttaacg gtgtagaacg cgagcctggg
    13321 gcgatacaga tgtgtctgac gccgtgacgc gttaatgctt ccagaatgac cgccgcccag
    13381 cgtcggttaa atgcgcttac tgacatgagt ttgtccggta tcaatattgc ggctaagtat
    13441 aaggagctac aaaaatagat tattgatatg aatcggtaat gatgcgactc attactattc
    13501 catttgtaat aaagtacgca gccctgccgc tttgttgtcg atttcctgcc actcttgctc
    13561 ggggtcggaa ccacggacaa tgcccgcgcc agcatatagc cgcacgacat tgccgctgat
    13621 ttttgctgaa cgcagggaaa cgcagaattc gctttgttgt aatgagagat agcccgcaga
    13681 accggcgtac cattctcggg tgaacggttc gtgacgggcg ataaactgtc gcgccagatc
    13741 gcgcggtaag ccagcaactg ctgccgttgg ctgcaactga tgtaaacaga tcacatcatc
    13801 cgctttgttg agtgaagtcc agatacagcg gcgaagatgc tgcactttac gcagacgcag
    13861 tacctgcggc ggtaaaacat ccagcgtctg ggtatcggcc tgtaatcgtt ggcagatatc
    13921 ttccaccacc agcatgttct cgcgctggtt tttatcatcc gccatcagcc actctcctaa
    13981 ctgctgcgcc tgcttatcat caggattatt tgctactgtt cccgccagcg cttcagtacg
    14041 cagcgctttg tcacgccgcc gccataaccg ttccggtgaa gagccaagaa aagcattttc
    14101 gccatcaaag gccatgtaaa aatggtagca attcagattc agtcgacgac tggcagccat
    14161 catcgccgcc gcgttgaccg gacttgcgaa atgcaggtca gttgcccgag cgagcaccac
    14221 tttgtcgagc tcaccttcgg cgatggtttt cgttgccagt tcgattaatt gcgtccagcc
    14281 cgttttgtcc ggccagtgtt gttctcgcgt ggtggttaaa tgtaacccag gcaagggctt
    14341 gatactcacc agtgtggcga taaattcttt tgcctgaatc gcatcgtgct gaagggagct
    14401 ttcgctgaat agcgtcagcc gcagcgtggc tttaccgcca cagcgtcgcc attccaggcg
    14461 gggtaaaagt aaattgccct gcgacgggtc aaaagcattc agcccccaaa tgcgtaagtc
    14521 ggcgtgttcc gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg taaaacgggt
    14581 aatcgcgccc aggacggctg cttcttcatc accattacgt tgttgccagt agaattgcgg
    14641 gtacgtttgc tgactggcca gccagctcaa ggcatcaaaa gcgtctttga gagggaaagg
    14701 aatatcgata acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat ggcgcagtag
    14761 attttccagc gccgtagtaa gtgattgcac gcggacctct ccctgttaaa agcctcacat
    14821 tatacggggt actacaaaaa aaatgcagta ccccggtgta gggaggtttt aacggcgtgc
    14881 cagcaacagt cctagtacca gcccaacggc cgcgcccaca ccaattcctt gccagggttt
    14941 ttcgtggacg tagtcatcag cacgataaac tgcctgcttc gcccgatagt aataactgtc
    15001 tgaagcctgg ctaacccgtt ttttcacatc atccagcgct ttttctgcac gcgctttcag
    15061 ctcaacatat ttctgatcgg cgggatcgcc agaggagcgg agcacctctt ccagtgtttc
    15121 actaagcagc gtcaggtcgt catcgatacg tgtatcacca aactgattag acattctcgt
    15181 tctccatttg cgtaaaacct gtcactaact atagacaagg gttgttgatt acgcctgaat
    15241 tacctcgcgt gccatcccaa tgtgtgggat accatcttct tcatagacct ccgtcacagg
    15301 gataaaaccg aaactctggt agaagttttg cagatgcgcc tgcgccccga ggtaaacagg
    15361 cttatcaggc cagtgatgcg tacatgtttc gagtgtttta ctcatcagct gctgccccac
    15421 tttttcgcca cgcaacgctt cactgacaat cacccgacct ataacgaccg gctcaagatc
    15481 atcatcactt ttcagaatcc tcgcatatgc caccagttca tcatttttcc aaccgagaat
    15541 atggcgatta tcccccgtca aatcatcacc atcgatatcc tgataagggc aattctgctc
    15601 gacaacaaac accgcgcaac gcagttgtaa taaggcataa agctgggaaa ccgataattc
    15661 agagtgatgc agatcttgcc attcaatcat aatgctctcc ttttagatgt atgccttata
    15721 ctagccggaa tattttttga aacgtgatga actcatggaa ttaatttttt taggtacttc
    15781 ggccggtgtg ccaacccgca cgcgcaatgt cacggcaata ttgcttaacc tgcaacatcc
    15841 gacccagagc ggtctttggt tgtttgactg cggtgaaggc acccagcatc agctactgca
    15901 taccgccttt aaccctggaa aactggacaa gatttttatc agtcaccttc atggcgatca
    15961 tctttttggt ttacccggct tgctgtgcag tcgttctatg tcaggcatta tccaaccctt
    16021 aacgatttat ggtccgcaag gtatccgtga atttgtggaa accgcgctgc ggattagcgg
    16081 ctcatggacc gattatccgc tggaaattgt cgaaattggc gctggcgaaa ttctcgatga
    16141 tggcctgcgc aaagtaaccg cttatccgct ggaacaccca ctggaatgtt atggctatcg
    16201 tattgaagaa catgataaac cgggtgcatt aaatgcccag gcattaaaag ctgctggcgt
    16261 gccgcctggc ccactgtttc aggaattaaa agcgggcaaa acaatcacgc tggaagatgg
    16321 aaggcagatt aacggcgcag attacttagc tgctccagtg ccaggtaaag cgctcgctat
    16381 tttcggcgat accggcccct gcgatgccgc acttgacctg gctaaaggtg tcgatgtcat
    16441 ggtgcacgaa gcgacgctgg atataaccat ggaagccaaa gccaatagtc gcggccatag
    16501 ctctacacgc caggctgcga cactagcccg tgaggctgga gtcggtaagc taatcattac
    16561 ccacgtcagc tcgcgctatg atgacaaagg ttgtcagcac ctgttacgtg aatgcaggtc
    16621 aattttcccg gcgactgaac tagcgaatga tttcaccgtg tttaacgttt aactctgctt
    16681 attattaaaa acagggcgaa acttgccctg ttatcgcaac ccgcgctaca tatatccacc
    16741 cacacgggca tattgagcga tttttgatat ttattcatct ccttacgcac tatattcaga
    16801 gcaataagtt gcctcttttt atcttcattc agcacaacct ttgcaactaa aaggagccca
    16861 tgatggttac agttgtcagc aattattgtc aattatctca aacccaactc agtcagacat
    16921 ttgcagaaaa atttactgtg accgaggaat tactgcagtc tttaaaaaaa acagcgttat
    16981 ccggagatga agaaagtatt gagttactgc ataatattgc gttaggttat gatgaatttg
    17041 gaaaaaaagc tgaagatatt ctttaccata ttgttaga
//
LOCUS       DAZFWL020000096        17039 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFWL020000096 DAZFWL020000000
VERSION     DAZFWL020000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.096"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            157..759
                     /gene="ais"
                     /locus_tag="ACHKNP_RS17025"
                     /old_locus_tag="pgaptmp_003405"
     CDS             157..759
                     /gene="ais"
                     /locus_tag="ACHKNP_RS17025"
                     /old_locus_tag="pgaptmp_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HGU4705518.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(798..1223)
                     /gene="nudI"
                     /locus_tag="ACHKNP_RS17030"
                     /old_locus_tag="pgaptmp_003406"
     CDS             complement(798..1223)
                     /gene="nudI"
                     /locus_tag="ACHKNP_RS17030"
                     /old_locus_tag="pgaptmp_003406"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HGU4705519.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1502..2044
                     /gene="yfaZ"
                     /locus_tag="ACHKNP_RS17035"
                     /old_locus_tag="pgaptmp_003407"
     CDS             1502..2044
                     /gene="yfaZ"
                     /locus_tag="ACHKNP_RS17035"
                     /old_locus_tag="pgaptmp_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HGU4705520.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2144..3346
                     /gene="yfaY"
                     /locus_tag="ACHKNP_RS17040"
                     /old_locus_tag="pgaptmp_003408"
     CDS             2144..3346
                     /gene="yfaY"
                     /locus_tag="ACHKNP_RS17040"
                     /old_locus_tag="pgaptmp_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HGU4705521.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            3410..3871
                     /locus_tag="ACHKNP_RS17045"
                     /old_locus_tag="pgaptmp_003409"
                     /pseudo
     CDS             3410..3871
                     /locus_tag="ACHKNP_RS17045"
                     /old_locus_tag="pgaptmp_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3912..4814)
                     /gene="rpnE"
                     /locus_tag="ACHKNP_RS17050"
                     /old_locus_tag="pgaptmp_003410"
     CDS             complement(3912..4814)
                     /gene="rpnE"
                     /locus_tag="ACHKNP_RS17050"
                     /old_locus_tag="pgaptmp_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HGU4705522.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(5008..6198)
                     /gene="glpC"
                     /locus_tag="ACHKNP_RS17055"
                     /old_locus_tag="pgaptmp_003411"
     CDS             complement(5008..6198)
                     /gene="glpC"
                     /locus_tag="ACHKNP_RS17055"
                     /old_locus_tag="pgaptmp_003411"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HGU4705523.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6195..7454)
                     /gene="glpB"
                     /locus_tag="ACHKNP_RS17060"
                     /old_locus_tag="pgaptmp_003412"
     CDS             complement(6195..7454)
                     /gene="glpB"
                     /locus_tag="ACHKNP_RS17060"
                     /old_locus_tag="pgaptmp_003412"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HGU4705524.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQVLIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7444..9072)
                     /gene="glpA"
                     /locus_tag="ACHKNP_RS17065"
                     /old_locus_tag="pgaptmp_003413"
     CDS             complement(7444..9072)
                     /gene="glpA"
                     /locus_tag="ACHKNP_RS17065"
                     /old_locus_tag="pgaptmp_003413"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HGU4705525.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9345..10703
                     /gene="glpT"
                     /locus_tag="ACHKNP_RS17070"
                     /old_locus_tag="pgaptmp_003414"
     CDS             9345..10703
                     /gene="glpT"
                     /locus_tag="ACHKNP_RS17070"
                     /old_locus_tag="pgaptmp_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HGU4705526.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10708..11784
                     /gene="glpQ"
                     /locus_tag="ACHKNP_RS17075"
                     /old_locus_tag="pgaptmp_003415"
     CDS             10708..11784
                     /gene="glpQ"
                     /locus_tag="ACHKNP_RS17075"
                     /old_locus_tag="pgaptmp_003415"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU4705527.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11826..12065)
                     /locus_tag="ACHKNP_RS17080"
                     /old_locus_tag="pgaptmp_003416"
                     /pseudo
     CDS             complement(11826..12065)
                     /locus_tag="ACHKNP_RS17080"
                     /old_locus_tag="pgaptmp_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            12063..12167
                     /locus_tag="ACHKNP_RS17085"
                     /old_locus_tag="pgaptmp_003417"
                     /pseudo
     CDS             12063..12167
                     /locus_tag="ACHKNP_RS17085"
                     /old_locus_tag="pgaptmp_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12247..12897
                     /gene="inaA"
                     /locus_tag="ACHKNP_RS17090"
                     /old_locus_tag="pgaptmp_003418"
     CDS             12247..12897
                     /gene="inaA"
                     /locus_tag="ACHKNP_RS17090"
                     /old_locus_tag="pgaptmp_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HGU4705528.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(12951..13205)
                     /gene="yfaE"
                     /locus_tag="ACHKNP_RS17095"
                     /old_locus_tag="pgaptmp_003419"
     CDS             complement(12951..13205)
                     /gene="yfaE"
                     /locus_tag="ACHKNP_RS17095"
                     /old_locus_tag="pgaptmp_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HGU4705529.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(13205..14335)
                     /gene="nrdB"
                     /locus_tag="ACHKNP_RS17100"
                     /old_locus_tag="pgaptmp_003420"
     CDS             complement(13205..14335)
                     /gene="nrdB"
                     /locus_tag="ACHKNP_RS17100"
                     /old_locus_tag="pgaptmp_003420"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU4705530.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(14424..16709)
                     /gene="nrdA"
                     /locus_tag="ACHKNP_RS17105"
                     /old_locus_tag="pgaptmp_003421"
     CDS             complement(14424..16709)
                     /gene="nrdA"
                     /locus_tag="ACHKNP_RS17105"
                     /old_locus_tag="pgaptmp_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU4705531.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            16837..16980
                     /gene="ypaB"
                     /locus_tag="ACHKNP_RS17110"
                     /old_locus_tag="pgaptmp_003422"
     CDS             16837..16980
                     /gene="ypaB"
                     /locus_tag="ACHKNP_RS17110"
                     /old_locus_tag="pgaptmp_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HGU4705532.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4011 a         4173 c         4559 g         4296 t
ORIGIN      
        1 ttttttctta tttaggcttt gcatttggca aaattttgag gcattttgcc gacatcgtag
       61 gatttttaat attacaccaa ctgctaatta tcgccagaaa tgtcgctcaa ttttacggta
      121 attgtctggt tgcgcttgtc tataggtgga gtttacgtgt tagctttttg ccgctcatca
      181 ttgaagtcaa aaaaatattt catcatttta ctggcgctcg ctgcaattgc cggactgggt
      241 actcatgccg cctggagtag caatggtttg ccacgtatcg acaataaaac attggccaga
      301 ctggcacagc agcacccggt tgtcgttttg tttcgccatg ctgaacgttg cgatcgttca
      361 accaatcaat gcttgtcaga taaaacaggt attacggtta aaggtaccca ggatgcccgt
      421 gaactgggca acgcttttag cgctgacatc cctgatttcg atctttattc cagtaatacc
      481 gtccggacca ttcagtcggc gacctggttt tccgcgggta aaaagctgac ggtagataaa
      541 cgacttcttc agtgcggtaa tgagatttat agtgcaatta aggacttaca aagcaaagcg
      601 cctgataaaa atatcgttat tttcacccat aatcattgcc tgacatatat tgctaaaaat
      661 aagcgtgatg cgacatttaa acctgattat ctggatggtt tagtcatgca tgtggaaaaa
      721 ggcaaagttt atctggatgg ggaattcgtt aaccactaaa ttttcaatct gacagccagt
      781 gatggctgtc atcattgtta aagaagacct ttcaaacgta acgtttttcg ggtggcaaca
      841 tttaaatcat aatgcaccag atcttcaggt tttacccacg cgtagtcctg aaactcttcg
      901 tttattttca cttctcggtt ggcagaaacg cagtcaaaaa tcaggtaaat catataaatc
      961 tcttccttgc gaccatctgc atacgtcttg gtgcgaatat catcgctgaa ggtccacggc
     1021 gtgatttctg tcaaaagcag ctgttctccc agttcttcgc gaatttcgcg gcgtagtgcc
     1081 tcttcaattc gttcgccaga ctccacgccg ccacccgaaa gcgcccattg accggggaaa
     1141 acgccgcgat cgtcggccat tttacacagc aaataagcac catcattttg aatcaaaggg
     1201 catacaatag tccgttgtcg cataggggta ttccttataa ataaaaagaa accatgagaa
     1261 tacggtaact cagaaatatg tgaaagtgac agtaatctct tttgcaatga taactgcact
     1321 tattattatt tttgtgtgta tcggtaaagt gtgtttattt tttcggcgtt taattattta
     1381 agaagtattt agttacaata agtgactaga tccctttttt cgctcattga atcctctgtg
     1441 ccaggcagta caatcccgca gctttgggaa ttttgattaa ttgcttgtgg gaaaggggat
     1501 aatgaaaaaa attgcgctag caggtctggc cggaatgctg ttggtttctg catcggtcaa
     1561 tgcaatgagc atcagcggcc aggcgggtaa agaatacacc aatattggtg tcggttttgg
     1621 gactgaatcg acgggcctgg ctttaagcgg taactggaca cataacgacg acgacggtga
     1681 cgtcgcgggc gtggggctgg ggttgaatct gcctctcggg ccgttaatgg cgaccgttgg
     1741 cggaaaaggc gtttacacca acccgaatta cggcgatgaa ggttatgccg cagcggtagg
     1801 aggtggtttg cagtggaaaa ttggcaacag cttccgtttg tttggcgagt attactactc
     1861 tccggattcg ctctccagcg gtattcaaag ttatgaggaa gcgaatgctg gcgcgcgtta
     1921 caccattatg cgtccagtca gtattgaggc gggttatcgc tacctgaatc tgtcgggtaa
     1981 agacggtaac cgcgacaacg ccgtggctga cggcccgtat gttggggtta acgccagttt
     2041 ctgatccctt ccggcgcggc attctgtcgc gccattcagt ttcttactac gccttatctc
     2101 cctttgcgtt atagtgattc gataacaaaa gcaggagata accatgttaa aagtggaaat
     2161 gctatccacc ggggatgaag tgttacacgg gcaaatcgtt gacactaacg ctgcctggct
     2221 ggccgatttt ttctttcatc aggggctgcc attatctcgc cgcaatacgg tgggggataa
     2281 ccttgatgac ttagtcacca ttcttcgcga acgtagtcag cacgccgatg tgctgatcgt
     2341 taacggcggg ctgggaccga ccagcgatga tttaagcgca ctcgccgctg cgacagcaaa
     2401 aggtgaaggc atggtgctgc atgaagcctg gctcaaagag atggaacgct atttccacga
     2461 acgtggacga gtaatggcac cgagcaaccg taaacaagcg gagctgcctg ccagtgctga
     2521 atttatcaat aacccggtag gcaccgcctg tggttttgcc gtgcagctta atcgttgcct
     2581 gatgttcttt actcccggcg taccgtcaga atttaaggtg atggtcgagc acgaaatcct
     2641 gccgcgtctg cgcgagcgtt tttctttacc acagccgccg gtttgtctgc gtttaactac
     2701 ctttggtcgt tcggaaagcg atctggcaca aagcctggac actctacaac tgccgccggg
     2761 cgtaacaatg ggctatcgct cttcaatgcc gattatcgaa ctgaaactca ccggaccggc
     2821 aagcgagcaa caggcgatgg aaaaactgtg gctggacgtt aaacgtgttg ccggacagag
     2881 cgtgattttc gaaggcaccg aaggactgcc cgcgcagatc agtcgcgaat tgcaaaaccg
     2941 ccagttcagc ctgacgttga gcgagcaatt caccggtggt ttattggctt tgcaactttc
     3001 tcgcgcaggt gctccattgc tggcgtgtga agtggttcct tcacaggaag aaaccctggc
     3061 gcaaactgcg cactggatta cagaacggcg ggccaaccat tttgccgggc tggcactggc
     3121 tgtttcgggt ttcgagaacg aacatctcaa ctttgcgcta gccacaccag acggcacttt
     3181 cgctctgcgt gtgcgtttca gcactacgcg ctacagcctg gctatccgtc aggaagtgtg
     3241 cgcaatgatg gcactgaata tgctgcgccg ttggttaaac ggccaggata tcgccagtga
     3301 gcatggctgg attgaggtta ttgagtccat gaccttatct gtctgattcc gccactctgt
     3361 gccggtttac tgcaccggca cattccccct ccggcaaaac ttgtccaacc ctccctacgg
     3421 tgagcgtggt gttggggcca gtgtggcacg ggctgcgcgc tggggacgca ttgagaatta
     3481 catggcgcaa gtcaacgatt cgctttgtct gttagtgcag gtggaaagta aaacggcact
     3541 ggataacctg gacgagatcc tcgacgtcga agggattgat ggcgtgttta ttggaccagc
     3601 ggatctttct gcgtcgttgg gctacccgga taacgccggg cacccggaag tgcagcgaat
     3661 tattgaaacc agcattcggc ggatccgtgc tgcgggtaaa gcggctggtt ttctggctgt
     3721 ggctcctgat atggcgcagc aatgcctggc gtggggagcg aactttgtcg ctgttggcgt
     3781 tgacacgatg ctctacagcg atgccctgga tcaacgactg gcgatgttta aatcaggcaa
     3841 aaatgggcca cgcataaaag gtagttattg atatcaaagg cccatgggga tcggctgtgg
     3901 gcctgtgtta attagtggtt attcgctgcc agatcggctt cgcttagctg ggtggccgcg
     3961 agcacctggt cacgatcaat gccctgttcc agcatgcgca aagcaatttt aatggcttgt
     4021 tcatgcatac cttcctgcca gccaatttga tgcccttcct gccgtagccg ttcagcaata
     4081 gtcattaatc tctccttttg tagtggtgaa cgttcggcaa tctcctcaat aaaacgggtg
     4141 aaacgggagg tatcgccgca ttgcagcaga taattaaaca gtgtttgtag ctggctgtca
     4201 ttagtgaagc ctctaaccaa aagcgtggtg atcctgtcga ccatgccgat taaatcgcgg
     4261 tcgcgaatat gcttttgaat cagttccagc agagctatac gccgatgttg catgatctca
     4321 tcgtcaggta cgatggtgat atccaccaac ggaaaagctt cggtgtacaa ctgccgggca
     4381 agttgcggat cgtcaaactc atccagccag ttaagtgagt acgggtaggg cgatgtttcg
     4441 ccatgataaa acagcaatgg caccaccagc ggaactctgt catagccttt atccaggtga
     4501 cgctgcatgg cggcagtggc atagcgcatt agccgaaaag ccatattctt ttctgcagag
     4561 ctttgatgtt caatcacgca gtagatatag ccgtcaccgt cgctggtttc cacggaccac
     4621 aaaacatccg agtagtaagc gcgtaaactt ttttcaataa aactagtggg ttccaggcgt
     4681 aaggtttgca ggttgcaaag cttgcgcagt ggttctggta aatgtatttc gagaaaatcc
     4741 cgtgcggttt cgggtgtgaa cataaaggtt ttaaacaccg catcatgcgg ggaggatgtt
     4801 gttgattcgg tcatggtttt ccgtcacctg atatttatga tgacgcgaca ttacgtctat
     4861 cccgggcctt tgacatctgt cagtttcagc ctttgcgaga cattttcaga ggaattttca
     4921 aagcgttgca acgcatacat ggtgcgtgga atcgcgctcg caaaaatgcc cgataagcaa
     4981 tgtgcttacc gggcatcaaa aggtcggtta agccagcgcc tgggccagta gcgtaatcgg
     5041 atgttcgcag cgaaggctcg tggacatctc aatctgccat ttgcaggttt cgcagtcggt
     5101 gatgaccaga tccgcgccgc tctcttctat ctggcggaac agtggcgcac cgatggcttg
     5161 tgaggtgggg tagttctctt ttttgaaacc gtaagtaccc gcaataccgc agcactggga
     5221 atccagcacc gttaactcaa gccccgggat tttacgcaac agctccaggg tgtagagcgt
     5281 ccagcccatt ttttccatat ggcacggagt gtgataaacc actttcagcg gcagcggttt
     5341 cagcggtaac gttttgcctt cgtccagctt gcgccacagc cagcgggttg ccagttcgat
     5401 atgatcgcgc aagcctttgt tgtcgacatt cagcacttcc gggtattcgt cgcgcagggc
     5461 aaatgtacag gttgaggagg tggcaatcac cggaatgcct tttactccca cagcttcgcg
     5521 gatcgactct acattcgtaa ttgcctgttt gcgtgcttta tcggtaaagc cgttggcgat
     5581 tagcggtacg ccgcagcatt tttctttgct gagcagttgt acaccggtac ccattgcgtt
     5641 gagcacttta attaaatctt tacctaactg cggatggttg tagttaacga agcagccgtg
     5701 aaagaaagcg acctggtctt tatattgtgc ttgctgagcc gctatgctgc gataccagcg
     5761 acgaaacgta ccgaaggagt atttcggtag cgtgcggcga tgatcgattt ttaacgccgc
     5821 atcaagcagc tgccgcaccg gtttcagcga ggtagcggtg ttgacgattg gtgcgaacgg
     5881 cgtggaaacg ctacccatca ggtcggtatg actcaacaca aaattacgca gcgacgggcg
     5941 cgtggtgtca tattttgccc gcgcgcgctg gataatatcg ccaatcttca catcggacgg
     6001 gcaggcgact tcacaacgtt tgcagttgat gcaatatttc agcgcctcgt catacagtgc
     6061 gccatctttc aaacgcagac gctcgccatc cggcccggct tgttttggcc ctggataacc
     6121 gggattcacc cggctcaccg ggcaggcggt ggtgcagacg gtgcacttaa tgcagttttc
     6181 gaagctggtg tcgttcattg ttggcctcct gcgcgttggg caatctgttg agcggcatgt
     6241 aaagcactga cggcacaaac accgccgccg catccctggg cgatgggatc aaatccgccc
     6301 agcaccgaac cgatggcaaa caggttctca atggtttgcc ctgcctgcga cgggcgtagc
     6361 gtctgatcag tggttacacc gaactgctgc cacggttgcg gcgcaaaaaa atcgccctta
     6421 taccattcac cccgcgtggc ggtttgtagc acatcaaggc cgagaatcgg ctcgcgaatg
     6481 ccgttacgtt ccgctaccag tccgccacta aagaaactgc cgctggcgag aaccgcgaaa
     6541 cgtggacgta gcggaatatc ggcgtgattg cgggtccaga tttcattcac tacgccattt
     6601 ttacaggtca cttttttcac ttcatcgccc ggcatccaca cgccaccctg gcgcacaaac
     6661 tggcgctgta actggttttg cagacgaatg cccagcacgg aaggcggcag cgttggcaaa
     6721 agcatcagtg aacaaggtag tttttcattc aaccaacgcc acagtttgtc atcggccagg
     6781 ccgaagcagg cgggcatcag gatcatttcg caggtattgg cgacaggaat aagcgcatca
     6841 agtaacagcg gccagttttc ttcattatca aggaaacggg cgatattcac cgcgcgaaat
     6901 tcggtggcgt tattgcgcag gacatccagt tccggcagct ctatttctgc ggtttcaacc
     6961 gaaagatcca gttcgcgcaa cgaagccgcc gcaagatgcg cctggaaatc catcagcccg
     7021 cttatcccca ctacacatat cttcttcgcg ggtagcggcc agacgggaac ttctggcgaa
     7081 cttaaccagg ttgcacgcaa agtgccgagc ggggtaacgc gctgatgggc cagttccacg
     7141 ctgccctgca attgcgcacc gctttcagcg atcaatacct gcgcctggca agcgagatcg
     7201 agcacgcgtt gtggttcgag aagactgtaa ggatgggctg gtgcctgctg acgcaaagat
     7261 tccagtccac tgtgaatgtc tgtcaccggt tgaccatcag gcagatggct cagcaaatcc
     7321 agcgaaccgg atgagaaatg cagcgcgctt tgaccacgcg tgacaatggc acagcgcagg
     7381 ccgtgttttt gcagttgcag gccacagagt aatccggcga ggccgccgcc cataatgaca
     7441 gtatcaaagc gcatctttct gctccttctc cagaccacac aatccctgat aaacccagcg
     7501 ggtaaattcg ctttcgcgca gtgcatctcc ccaggcgatg ggttgcacgc ctttccagcg
     7561 ttcgttaagg aaggtggaaa gttgctcgat agattgcgcg gacgtcgtga cgttaaaacg
     7621 ttgcagcagt ccggcagcgc ggcaggcgca gagttcgccc tggcaggtac ccatccccac
     7681 acgggtacga cggcgtaaat ccagcaggct attaacgttt aaattttcta ccgcgtactg
     7741 aacttcaccc gcagttaccg cttcgcactc acataccagg ctacgatgca gacggccttc
     7801 gctcagccag gcaggcgtgc gatcgccatg acgataaacc gcagaaccgc gcagcggggc
     7861 aggcagggag atgactttac gcaaggtaac ttcagcgggt tcttgtgaac caggcagtgc
     7921 cagatcggca gtcgtacagg ggcgcgtgtt gcccagtttg cggcataccg cgtcggtagc
     7981 ccattcagcc atcagccgat aggtcatcag tttgccaccg gtgatggtga taaatccgtc
     8041 cagaccatcg cgttcagcat ggtcgagcag cacgatgcca cggctgacgt tacgtccgct
     8101 cgggtcgtca tcgctggcaa ccagcgggcg cacgccagaa taggcccgca aaatgcgcgt
     8161 tttcgccatc acgggggcca gtttttcccc ttcacgcagc agaatatcaa cctcttctgc
     8221 cgtcactcga ttatcgtcaa tctcgttgta atcaatacgt aaagaggtgg tgccaatcag
     8281 cgaaatggta tcgccaggca ccagaatatc ggcgtcggaa ggtttacggc agcggttgat
     8341 cacatgctgg ttaatgcggt gatccatgat cagcagcgat cctttcgccg ggaacatgcg
     8401 aatgcgcaga tcggcatatt cggcaatgtg ttgcccccag atcccagcgg cattaaccac
     8461 gacaggtgca tgaagggcct gagtttcgcc ggtgagatgg ttacgtacac gaacaccgca
     8521 caccgtcgcg ccttcacgaa tcagccccgt gacttcatga gcggtaagga taacggcacc
     8581 gtgttctttg gcatccagca tgtttgctgc ggtcagacga aatggatcaa cggtgccatc
     8641 cggaactttc accgcgccaa tcagtgccgg gttaacggca ggttcgataa tgcgcgcttg
     8701 ctgcgggtct atagcttctg cgctgatccc tgcttcttcg caggcgcgaa taaaagtggc
     8761 ctggaatgag aggtcatctt ccggcagggt gataaacagg ccgttggttg gttcaacgca
     8821 gtgacgtgca atgcgtttca ggatctggtt ttcactaatg cattcgcggg ccgattccgc
     8881 atcggttacc gcatagcgcg caccgctgtg cagcaggccg tggttacgcc cggtggcacc
     8941 ggttgcgatg tcgtggcgct caaccaaaat cacgcgcagc ccgcgcaggg cacagtcgcg
     9001 ggcaatcccg gctcccgttg cgccgccacc aatgataatc acgtcacttg attgcgagtc
     9061 gcgagttttc attgttatcc ctctgaagtt cgttttttac catttagcca tagtaaaaac
     9121 atgaattgtt tgatttcgcg catattcgct cataattcga aagtgaaacg tgatttcatg
     9181 cgtcattctg aacattttgt aaatcttatt taataatgtg tgcggcaatt cacatttaat
     9241 ttctgaatgt tttcttaaca tcgcggcaac taaagaaacg gcaggttctc ccactgaatc
     9301 agtctgttaa tcataaataa gaccacgggc cacggaggct ttcaatgttg agtattttta
     9361 aaccagcgcc acacaaagcg cgcttacctg ctgcggagat cgatccgact tatcgtcgat
     9421 tgcgctggca aattttcctg gggatattct ttggctatgc ggcttactat ttggttcgta
     9481 agaactttgc gcttgctatg ccttatctgg tcgagcaggg attctctcgc ggtgatttag
     9541 gtttcgcgct ttcggggatc tcgatcgctt atggattttc gaaattcata atgggttcgg
     9601 tatcggatcg ctcgaatccg cgcgttttcc tgcccgcagg tttgattctg gcggcggcag
     9661 tgatgttgtt tatgggcttt gtgccatggg cgacgtcgag cattgcggtg atgtttgtac
     9721 tgttgttcct ctgcggttgg ttccagggga tggggtggcc gccgtgtggt cgtactatgg
     9781 tgcactggtg gtcgcagaaa gaacgtggcg gcattgtgtc agtgtggaac tgtgcgcaca
     9841 acgtcggtgg tggtattccg ccgctgctgt tcctgctggg gatggcctgg ttcaatgact
     9901 ggcatgcggc gctctatatg cctgctttct gcgccattct ggtggcatta ttcgcctttg
     9961 cgatgatgcg cgataccccg caatcctgtg gcttgccgcc gatcgaagag tacaaaaatg
    10021 attatccgga cgactataac gaaaaagcgg aacaggagct gaccgcgaag caaatcttca
    10081 tgcagtacgt gctgccgaac aaactgctgt ggtatatcgc catcgccaac gtgttcgttt
    10141 atctgctgcg ttacggcatc ctcgactggt caccgactta tctgaaagag gttaagcatt
    10201 tcgcgctgga taaatcctcc tgggcctact tcctttatga atatgcaggt attccgggca
    10261 ctctgctgtg cggctggatg tcggataaag tcttccgtgg caaccgtggg gcaaccggcg
    10321 ttttctttat gacactggtg accatcgcga ctatcgttta ctggatgaac ccggcaggta
    10381 acccaaccgt cgatatgatt tgtatgattg ttatcggctt cctgatctac ggtcctgtga
    10441 tgctgatcgg tctgcatgcg ctggaactgg caccgaaaaa agcggcaggt acggcagcgg
    10501 gctttaccgg gctgtttggt tacctgggcg gttcggtggc ggcgagcgcg attgttggct
    10561 acaccgtgga cttcttcggc tgggatggcg gctttatggt aatgattggc ggcagcattc
    10621 tggcggttat cttgttgatt gttgtgatga ttggcgaaaa acgtcgccat gaacaattac
    10681 tgcaaaaacg caacggaggc taatggcatg aaattgacgc tgaaaaacct tagcatggcg
    10741 atcatgatga gcactatagt catgggaagc agtgcaatgg cggcggacag caacgaaaaa
    10801 atagtcatcg cccatcgcgg tgccagtgga tatttgccgg agcatacgct gccagcaaaa
    10861 gcgatggcgt atgcgcaggg agcggattat ctggaacagg atttggtgat gaccaaagac
    10921 gaccatctgg ttgttctgca tgaccattat ctcgatcgtg ttactgatgt tgccgatcgt
    10981 ttcccggacc gggcgcgcaa agacggtcgt tactacgcga tagatttcac gctggatgaa
    11041 attaagtcgc tgaaatttac cgaaggtttc gatattgaaa acggtaaaaa agtgcagact
    11101 tatccggggc gtttcccaat gggtaagtcc gacttccggg tgcacacctt tgaagaagag
    11161 attgaatttg ttcaggggtt aaatcactct accgggaaaa atatcggtat ttatcctgaa
    11221 atcaaagcgc cgtggttcca tcatcaggaa gggaaggata ttgcggcaaa aacgctggaa
    11281 gtgctgaaga aatatggtta caccggtaag gacgacaaag tttatttgca atgttttgat
    11341 gctgatgagc tgaagcgtat taagaatgag ctggaaccta aaatgggcat ggatctcaat
    11401 ctggtacagt tgattgccta taccgactgg aatgaaacgc agcagaaaca gccggatgga
    11461 agctgggtta attacaacta cgactggatg tttaagccgg gtgctatgaa acaggtggcg
    11521 gaatatgccg acggaatcgg gccggattac catatgttga ttgaggaaac atcgcagccg
    11581 ggtaatatca aactcactgg catggtgcaa gatgctcagc agaataaact ggtagtgcat
    11641 ccttataccg tgcggtcaga taaactgcct gaatacacta ctgatgtgaa tcagttatat
    11701 gatgctctgt ataacaaagc gggtgtaaat gggctgttta ctgatttccc tgataaggca
    11761 gtaaaattcc ttaataaaga gtaatgctac tgcaggatgg cgttcatgcg ccatcctgtc
    11821 tcacatcaaa tttttccatt caccacgagt tgttccgtac acgtttgtag taaaaacagt
    11881 ggactgcctt ctaccggctt ttctttagcc aacaacgaaa tctgtcgata gaaggttggt
    11941 tcgtgaggaa cggaacacaa ttcacctgca atgcttttca gcgttaactc tggttgtaag
    12001 gcgatgccta atccctgtcg aataaacttc atcgctgtat ccgactgatt aaattcacat
    12061 tttatctact gggcgcacta ttagtggtag tggctggtgg aatctatgcg ctgcaaatga
    12121 agggtaaaag cggtgtcacg ctagtagtgg caaaagaaat tcattaatac gacacgtttc
    12181 attaagattt tcctcaggta accacctata gtcattgtcg ttaataaaaa tgacaggcga
    12241 tagggtatgg cagtttcagc aaagtacgac gaatttaatc actggtgggc aacagagggc
    12301 gactgggttg aagagcctaa ctatcgccgt aatggtatga gcggggtgca atgcgtcgag
    12361 cgcaacggca aaaagctgta tgtaaagcgc atgacgcatc atctgtttca ttccgtacgt
    12421 tatccgttcg gccgaccaac gattgtccgt gaggttgcgg taattaaaga actggaacgg
    12481 gccggtgtca tcgtgccgaa gatcgttttt ggtgaagcgg tgaaaattga gggtgaatgg
    12541 cgagcgttgc tggtgactga agatatggcg gggttcatca gcattgctga ctggtatgcc
    12601 cagcatgcag tatcgcctta ttctgacgaa gtacggcaag ccatgttgaa agcagtggcg
    12661 ctggcgttta agaaaatgca tagcattaat cgtcagcatg gctgttgtta tgttcgccat
    12721 atctatgtga aaacagaagg taatgcagaa gctggttttc ttgacctgga gaaaagtcgt
    12781 cgccgcttgc gtcgcgataa agcgattaat catgacttcc gccagctgga aaaatatctg
    12841 gagccaatcc ccaaagcgga ctgggagcag gtgaaagcgt attactacgc catgtaatcg
    12901 ttgtaggcca gataagacgc ataagcgttg tatctggcac tacaaccaat tcacatctcg
    12961 atttcaatat cgccttttgc ccggcaacaa cagggcaaaa tttcccccgg ctgaataaag
    13021 gctaacggtt cggcaatcca gtcaacctga cctgcaacca gacgggtgcg acaggagccg
    13081 cagtaacctt cgcgacactg atactcaacc gccacattgt gggattccag cgccgccaga
    13141 agggaagggt gttcatcctg gcacagcagt tgtgtgccag tgatgcgcag ggtaacgcgg
    13201 gccatcagag ttggaagtta ctcaaatcgt cggtgtccac ttccgcgtca atctgcccga
    13261 ccagataaga actgacttcc acttcctgcg gagcaacctg cacgttatca gacaccagcc
    13321 aggtgttgat ccatgggatc gggttggagc gcgtctggaa cggcagatcc aaaccgactg
    13381 cctgcatacg gatattggtg atgtattcaa cgtactggca gagaatgtct ttattcagac
    13441 caatcatcga accgtcgcgg aacagataat ccgcccagtc tttctcctgt tgagctgcct
    13501 gaacaaacag gtcatagcac tcctgcttac actcttcggc aatttccgcc atctcaggat
    13561 cgtccgcgcc gctgcgcagc agattcagca tatgctgggt gccggtcagg tgcagggctt
    13621 cgtcgcgggc aatcaggcga ataattttgg cgttgccttc catcaattcg cgttctgcaa
    13681 atgcgaagga acaagcaaag ctgacgtaga aacgaatcgc ttccagcgcg ttaacgctca
    13741 tcaggcagag atacagtttt ttcttcagct cgcgcaggct aacggtcaca gttttaccgt
    13801 taacggtgtg ggtaccttcg cccagcagat gccagtagct ggtcatttcg atcagctcat
    13861 cgtaatagct ggagatccct tccgcacgtt tctggatctg ctcgttggtg acgatatcgt
    13921 caaacacaac agacggatcg ttaacgatat tacgaatgat atgagtatag gaacgggaat
    13981 gaatcgtttc tgagaacgcc caggtttcga cccaggtctc cagttccggg atcgagatca
    14041 gcggcagcag tgccacgttc gggctacggc cctgaatgga atccagcagc gtctgatatt
    14101 tcaggttgct gataaagatg tgtttttcgt gttcaggcag tgcctggtaa tcgatacggt
    14161 cgcgagaaac gtcaacttct tccggacgcc agaagaaaga gagctgcttt tcgatcagct
    14221 tttcgaagat gtcatatttt tgctgatcgt agcgagccac gttgaccggc tgaccaaaga
    14281 acatcggttc tttgagctga tcatttttcg tctgtgaaaa ggtggtatat gccatgagtg
    14341 tgtcctgtgg ttttattacc cctcacccta accctctccc gcaaggggag aggggacggt
    14401 ttggtgcaaa ggctggggga ttatcagatc ttacatgcgc cgctttcgca gccatcgtcc
    14461 tggattgacg gtaccagatc gtcctgcgcg tcttcagcgc cgtcacgggt gttctgataa
    14521 tacagcgttt tgaccccgaa tttgtaggcg gtgagcaggt ctttcagcaa ctgctgcatc
    14581 ggcacttttc ctgacgggaa acgtgacgga tcgtagttgg tgttagcaga gatcgactga
    14641 tcgataaatt tctgcatgat acctaccagt tgcaggtagc catcgttacc cggcatttcc
    14701 cacagcagct cataggcgtc gtgcaggtgc tcgtagtccg gcaccacctg gcgcaaaata
    14761 ccgtctttcg acgctttgat gctgacgtaa ccgcgcggcg gttcaatacc gttagtggcg
    14821 ttagagatct gtgaagaagt ctctgacggc atcagggcag aaagcgtgga gttacgcagg
    14881 ccgtgcgttt tgattgattc acgcaatgct tcccagtcgt aatgcagcgg ctcattagcg
    14941 atggtatcca gatctttctt ataggtatcg atcggcagga tccctttcgc gtaagtggtt
    15001 tcgttaaacc acgggcacgc gccttgctct tttgccagct cgttagacgc tttcagcagg
    15061 taatactgaa tcgcttcgaa ggttttatgc gtcaggttgt tggcgctgcc gtcggagtaa
    15121 cgcacaccgt gcttcgccag gtagtaagcg aagttgatca caccaatacc cagcgtacga
    15181 cgacccatcg ctccacgttt ggcggccggg atcgggtaat cctgataatc cagcagcgcg
    15241 tcaagtgcac gaaccgccag aattgccagc tcttccagtt catccaggct attaattgcg
    15301 cccaggttga aagcagacag cgtacacagc gcgatttcac cgttctcgtc gttgacgtcg
    15361 ttcagcggtt tggtcggcag ggctatctcc aggcacaggt tagactgacg cactggcgcg
    15421 atggccggat caaacgggct atgggtattg cagtggtcaa cgttctgaat atagatacga
    15481 ccggtagacg cacgttcctg catcatcagc gagaacagct caacggcttt cacacgctgc
    15541 ttgcggatgc tgtcgtcttt ctcatatttg gtatacaggc gttcaaactc ttcctgatcg
    15601 gcgaagaatg cgtcgtacag ccccggtacg tcggacgggc tgaacagggt gatatcttca
    15661 cctttcagca ggcgggtata catcagtttg ttgatctgta ccccgtagtc catatggcgc
    15721 acgcggttgc cttccacacc acggttgttt ttcaacacca gcaggctttc cacttccaga
    15781 tgccacatcg ggtagaacag cgttgccgca ccgccgcgca cgccgccctg agagcaggat
    15841 ttcaccgctg tctggaaatg tttgtagaac ggaatacagc cggtatggaa cgcttcacca
    15901 ccgcgaatcg ggctacccag cgcacgaata cgcccggcgt tgatgccgat cccggcacgc
    15961 tgggaaacgt atttcacaat cgcgctggag gtagcgttga tggaatccag actgtcaccg
    16021 cactcgatca gtacgcagga gctgaactga cgagtcgggg tacgcacgcc ggacatgatt
    16081 ggcgtcggca gcgaaatttt aaatgtggaa accgcgtcgt aaaaacgctt cacatattgc
    16141 aggcgcgttt cacgcgggta gttcgagaac aggcacgcgg caaccagaat ataaaggaac
    16201 tgggcgcttt cgtagatttc gccggtcacg cggttctgta ccagatattt gccttccagc
    16261 tgcttaacgg cagcataaga gaaggtcata tcacggtcgt gatcgataaa cgtgtccatc
    16321 tgcttgaact cttcttccgt gtagtcttcc agcagatgat tatcgtattt gcccatctcg
    16381 accattttca ccacgtggtc gtacagcgca ggcggctcaa actggccgta ggctttttta
    16441 cgcaggtgga agatcgccag gcgcgcggcg agatactgat aatccggcgc atcacgggag
    16501 atcaggtctg cggccgcctt gataatggtt tcgtggatgt cagaggtctt gataccgtca
    16561 taaaactgaa tgtgggagcg cagctcgacc tgggaaatcg aaacgttatg cagtccttct
    16621 gccgcccaat ccagaacgcg atggattttg tcgagattga tgcgctctgt gctaccgtcg
    16681 cgctttgtca ccagcagatt ctgattcatg tatgtcgtac ctgtttttgg aattctttcc
    16741 gcaatagttt catgcagact acctgtagta cgggaagctg cttagaacac tatatatagg
    16801 gggtatgttt gggattcact gcaagatagt gtgaaaatgg ccctcttgca agtgcataac
    16861 tttgtggata actcaggaag gaaaaagtgg ctttcgcgca ccttaggtca gacaaggtgt
    16921 ccgggaaagt caatgggaag agaaaaattt gttaaaaata actgttcggc gatttcttga
    16981 acggcagaat aggttggata aattgatata gatggtttat taatcttttt gctaatgat
//
LOCUS       DAZFWL020000097        16857 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFWL020000097 DAZFWL020000000
VERSION     DAZFWL020000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.097"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            88..1059
                     /gene="yfbL"
                     /locus_tag="ACHKNP_RS17115"
                     /old_locus_tag="pgaptmp_003423"
     CDS             88..1059
                     /gene="yfbL"
                     /locus_tag="ACHKNP_RS17115"
                     /old_locus_tag="pgaptmp_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HGU4705533.1"
                     /translation="MKKINFAFIILFLFSLPLIIFYQPWVNALPPMPRHANPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFVSSGARITSQDVPITGGPYKNIVADYGP
                     ADGPLIIIGAHYDSASSYENDELTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSAPGSQDYPYPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKATLLSSRYLSVYSMNAPGFIPGIDFSDHLNYWQHD
                     IPAVMITDTAFYRNKQYHLPGDTADRLNYQKMAQVVDGVINLLYNSK"
     gene            1162..1665
                     /gene="yfbM"
                     /locus_tag="ACHKNP_RS17120"
                     /old_locus_tag="pgaptmp_003424"
     CDS             1162..1665
                     /gene="yfbM"
                     /locus_tag="ACHKNP_RS17120"
                     /old_locus_tag="pgaptmp_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HGU4705534.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1732..3189)
                     /gene="nuoN"
                     /locus_tag="ACHKNP_RS17125"
                     /old_locus_tag="pgaptmp_003425"
     CDS             complement(1732..3189)
                     /gene="nuoN"
                     /locus_tag="ACHKNP_RS17125"
                     /old_locus_tag="pgaptmp_003425"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HGU4705535.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3196..4725)
                     /gene="nuoM"
                     /locus_tag="ACHKNP_RS17130"
                     /old_locus_tag="pgaptmp_003426"
     CDS             complement(3196..4725)
                     /gene="nuoM"
                     /locus_tag="ACHKNP_RS17130"
                     /old_locus_tag="pgaptmp_003426"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HGU4705536.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFDISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSRSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4889..6730)
                     /gene="nuoL"
                     /locus_tag="ACHKNP_RS17135"
                     /old_locus_tag="pgaptmp_003427"
     CDS             complement(4889..6730)
                     /gene="nuoL"
                     /locus_tag="ACHKNP_RS17135"
                     /old_locus_tag="pgaptmp_003427"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HGU4705537.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLVT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6727..7029)
                     /gene="nuoK"
                     /locus_tag="ACHKNP_RS17140"
                     /old_locus_tag="pgaptmp_003428"
     CDS             complement(6727..7029)
                     /gene="nuoK"
                     /locus_tag="ACHKNP_RS17140"
                     /old_locus_tag="pgaptmp_003428"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HGU4705538.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7026..7580)
                     /gene="nuoJ"
                     /locus_tag="ACHKNP_RS17145"
                     /old_locus_tag="pgaptmp_003429"
     CDS             complement(7026..7580)
                     /gene="nuoJ"
                     /locus_tag="ACHKNP_RS17145"
                     /old_locus_tag="pgaptmp_003429"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HGU4705539.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7592..8134)
                     /gene="nuoI"
                     /locus_tag="ACHKNP_RS17150"
                     /old_locus_tag="pgaptmp_003430"
     CDS             complement(7592..8134)
                     /gene="nuoI"
                     /locus_tag="ACHKNP_RS17150"
                     /old_locus_tag="pgaptmp_003430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HGU4705540.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8149..9126)
                     /gene="nuoH"
                     /locus_tag="ACHKNP_RS17155"
                     /old_locus_tag="pgaptmp_003431"
     CDS             complement(8149..9126)
                     /gene="nuoH"
                     /locus_tag="ACHKNP_RS17155"
                     /old_locus_tag="pgaptmp_003431"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HGU4705541.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9123..11849)
                     /gene="nuoG"
                     /locus_tag="ACHKNP_RS17160"
                     /old_locus_tag="pgaptmp_003432"
     CDS             complement(9123..11849)
                     /gene="nuoG"
                     /locus_tag="ACHKNP_RS17160"
                     /old_locus_tag="pgaptmp_003432"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HGU4705542.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGA
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11902..13239)
                     /gene="nuoF"
                     /locus_tag="ACHKNP_RS17165"
                     /old_locus_tag="pgaptmp_003433"
     CDS             complement(11902..13239)
                     /gene="nuoF"
                     /locus_tag="ACHKNP_RS17165"
                     /old_locus_tag="pgaptmp_003433"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HGU4705543.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13236..13736)
                     /gene="nuoE"
                     /locus_tag="ACHKNP_RS17170"
                     /old_locus_tag="pgaptmp_003434"
     CDS             complement(13236..13736)
                     /gene="nuoE"
                     /locus_tag="ACHKNP_RS17170"
                     /old_locus_tag="pgaptmp_003434"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HGU4705544.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13739..15541)
                     /gene="nuoC"
                     /locus_tag="ACHKNP_RS17175"
                     /old_locus_tag="pgaptmp_003435"
     CDS             complement(13739..15541)
                     /gene="nuoC"
                     /locus_tag="ACHKNP_RS17175"
                     /old_locus_tag="pgaptmp_003435"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HGU4705545.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15635..16297)
                     /gene="nuoB"
                     /locus_tag="ACHKNP_RS17180"
                     /old_locus_tag="pgaptmp_003436"
     CDS             complement(15635..16297)
                     /gene="nuoB"
                     /locus_tag="ACHKNP_RS17180"
                     /old_locus_tag="pgaptmp_003436"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HGU4705546.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16313..16756)
                     /gene="nuoA"
                     /locus_tag="ACHKNP_RS17185"
                     /old_locus_tag="pgaptmp_003437"
     CDS             complement(16313..16756)
                     /gene="nuoA"
                     /locus_tag="ACHKNP_RS17185"
                     /old_locus_tag="pgaptmp_003437"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HGU4705547.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
BASE COUNT         4186 a         4744 c         4411 g         3516 t
ORIGIN      
        1 gtataatcct gctaatctta ataagatgca atatcgaaac ccataacaaa taatgattca
       61 ataacgaaac ccattaataa tttgcatatg aaaaaaataa attttgcttt tattatatta
      121 tttctgtttt cactccctct tattattttt taccaaccct gggtcaatgc attaccgcca
      181 atgccccgac acgcaaatcc tgagcaatta gaaaaaacag tgcgttatct tacacaaact
      241 gtgcatccac gtagtgccga caatattgat aatctgaata ggtccgcaga gtatataaaa
      301 gaggtcttcg tcagtagcgg tgccagaatt acctcgcagg acgtccccat tacgggtggc
      361 ccctacaaaa acattgttgc tgattatggt cctgccgatg gaccgctgat tattattggt
      421 gcgcactatg actctgccag cagttatgaa aacgatgaat tgacctatac acctggcgcg
      481 gatgataacg ccagcggcgt ggcaggatta ctcgaactgg cacgtttgtt acatcagcaa
      541 gtaccgaaaa caggcgtgca gctggtcgcc tatgcgtcgg aagaaccgcc cttctttcgt
      601 agcgatgaaa tggggagcgc ggtacatgca gcttcgcttg agcgtccagt aaaattaatg
      661 atagcactgg agatgattgg ctattacgac tctgcgcctg gtagccagga ttacccttac
      721 ccggcaatgt cctggcttta tcccgatcgg ggagacttta ttgccgtggt cggcagaatg
      781 caggatatca acgccgttcg tcaggtaaaa gcgacattgt tatcatcccg gtatttatct
      841 gtttattcta tgaatgcccc agggtttatt cccggtattg atttctctga tcaccttaat
      901 tattggcaac acgatattcc cgccgtaatg attactgaca ccgcttttta tcgtaataaa
      961 caataccact tgcccggtga taccgcagac agattgaatt atcagaaaat ggctcaggtg
     1021 gtggatggtg ttataaattt gttatacaac agtaaataat aaaaatttct cgaggcgggt
     1081 ccagaaaaag cgacatgcac attttatttt ccctcgtctg ttttaagatg cgaaacatga
     1141 aattcaatgg aggaaaaata aatgggaatg attggttatt ttgcagaaat tgactcagaa
     1201 aagattaacc aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg
     1261 ctttcaggat tacgccgact ggatattgat aaacgctggg attttttaca ctttgggctg
     1321 acaggcacct ctgcttttga tcccgcgaag aatgatccgc tgagtcgtgc cgtgcttggt
     1381 gaacacagcc tcgaagatgg catagatggc ttcctgggat tgacctggaa tcaggagtta
     1441 gccgcgacaa tcgaccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc
     1501 attaagcgac tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag
     1561 gggcaactct ttgcttcgat catgcttgat atggaaaagc tgattagcgc gtatcgacgt
     1621 atgctgcgac aagggaatca tgcgctgacg gttattgttg ggtgatggtg tcagggcacc
     1681 aaaaaaaacc gccgaacatg tcggcggctt tctgacttac aaactaacag attacatcag
     1741 cggcattgct aaccgcacaa tgctaatcag cggttgtggc catacaccca gcaccagtac
     1801 caacagtgca gaaatcagca ccacgatacc gcccgcgctg tactgccagt ttgatggtgc
     1861 atcgcgaccc ggttgttccg gggcgtgaag atacaggctc accgccacgc gcaggtagta
     1921 gtagaggccg attgccgaac cgacaaccac ggcacccacc agccaccaca agtgtgcctg
     1981 gacaccgacc gccagcacgt agaacttacc gataaagccc agcgtcatcg ggataccggc
     2041 cagagacagc atcatcaccg tcatcactgc cgcgaggatc ggacgatgcc agaacagacc
     2101 gcggtaagag aacagggaat cagcatccgg gccacgatac gggctggaca tcaggctgac
     2161 cacgccgaac gcgccgaggc tgctgaacag ataaccggcc aggtaaaccc ctaccgcttc
     2221 catcgacatc tcaccagttt gcagcgcaat cagcgccacc agcagatagc cgaggtgaga
     2281 gatagatgag taaccgagca gacgtttgat gttggtctgg ctcagcgcca tcaggttacc
     2341 gaagatgatg gaggcaaagg cgataatcgc cagtactacg cgaatcgctt cgctgtcacc
     2401 caccggtgcg tagaggaaca gacgcatcac cacaccgaag atagcgattt tgctcgccgt
     2461 cgccaggaaa gtggaaaccg gcgcaggcgc gccctggtat acgtctggcg tccacaggtg
     2521 gaacggcacc agcgagagtt tgaagccgag gccaacaatc atcagtccga aacctgccag
     2581 caacagcggc tcgttgagca taccgtcgcc gaggttttta cccagcgcga caaacgacag
     2641 gtcgccagac tgtgcataca ccagcgccat accaaacagc aggaaagaag acgctgcggc
     2701 agaaaggatg gtgtatttga tactggcttc cagcgaacgt ttctggcgga aagcgtaacc
     2761 gaccaggcca aacagcggca aagagatcag ttcaataccg aggaacaaag acgccagatg
     2821 gttggcattc gccagcagga tcccgcccag cgcggcaatt aacaccaaca ggtagaactc
     2881 atccttgttg tcgttatagc cttcaagcca cgggtaggcg aaagtacagg tggcgaggct
     2941 cgccaacaat accagcccgg tgtaaagcat ggcgaaacca tcaacgcgca tcagcggcgt
     3001 aacgtccata gcgcccgcct ggccgacaaa ccagagcgaa accagcgccg cgttaagccc
     3061 aataaccgag agcgtagcgt tgaggaaatg attgcgtcgc cacgcaatgg agagcatcac
     3121 aaccaccacc gtcaagccga cgatcagcaa cggtagcagt gcgatcaggt tttgtggagt
     3181 tattgtcatg gcgatttacg gccttgtagt agtaacggaa ttaacaaacc actgctggat
     3241 attgccaatc gcggagcgcg aggtatccag aatcggctgc ggatagaagc ccagcagtac
     3301 cagcagcacc accagcaaca ggatcataaa cagctcacgc agcgacatcc ctggcagttc
     3361 ctggctggca atctggcttt tcgctttacc gaagtaagcg cgatgcaaca tcgccagcga
     3421 ataaacagat gcaaagacca gcccaaaggt agagataacg gtaatcaccg ggacaacctg
     3481 gaagctgccg aacagaatca taaattcgcc gacgaagtta ccggtgccag gcatcccaag
     3541 cgttgccacc gcaaagaaca gcgacagtgc tggcagccat ttcatcttgc tccacagacc
     3601 gcccatcatg cgcatgtcgc gggtatggat acgttcataa agctgaccac aaagaataaa
     3661 cagacccgcc gccgacaagc cgtgcgcaat catctggatt accgcgccct ggtaggccaa
     3721 ctggctgccg gtgtagatag caatcagcac gaagcccatg tgggaaaccg aggtgtaggc
     3781 gatcagacgt ttgatatcgg tctgggcgaa ggccatccac gcaccgtaga agatgccgat
     3841 aacacccagc cacatagcaa ttggcgcgaa ctctgccgac gcgttcggga acagcggcag
     3901 ggagaaacgc agcaaaccgt aagcggcagt tttcagcaag atccccgcga ggtcaacgga
     3961 accggcggtc ggagcctggg agtgcgcatc cggcagccag ccatgcagcg gaaccaccgg
     4021 cattttgact gcgaaggcga tgaagaagcc cagcattaac aggtattcca caccactgga
     4081 cattggcgta ttcagcagct cttcatagtt gaaggtccag acgccggtcg cattgtagtg
     4141 aacaaaaacc agcgccagga tggcaatcaa catcaccagg ccactcgcct gggtgtaaat
     4201 gaagaacttg gttgccgccg tgatacgcgt tttaccgtca gaggctttat gcccccacag
     4261 tgcgatcagg aagtacatcg gcaccagcat catttcccag aagaagaaga acaggaacat
     4321 gtcgatggca aggaacacgc cgataacgcc gcccaggatc cacatcaggt tgaggtggaa
     4381 gaagccctga tatttttcga tctctttcca cgaacatagt accgccagca caccgagcag
     4441 accggtcagc acgaccatca gcagcgacag cccgtcaatg gcgagatgaa tagagatatc
     4501 aaaacgcggg atccacggca tgtcgaattc agactgccac tgcggaattc cggcggattg
     4561 cgtcagtgaa taaccgccct gcaaccacag ttgcagcgac agcgccagcg tcaatcccat
     4621 ggtgatcagc gcgatccagc gtggcacctt gacgccaaag cgttcggtct gccagcacag
     4681 gaagccgcca ataaagggaa ttaatattag ccagggtagt aacatggcga tctttattcc
     4741 ttgtaaaagt cccgtcagga ccggattttc aacgaattct cacgacagaa aattctcttc
     4801 ggactggggg tagtgtcgga tgcaccgcga aggcggctta tccgacctac gttcttatat
     4861 gcccaggggc aatcccacaa tccttaactc aacgcagtac catcaacagt gccagcacca
     4921 cgaccgcacc gatgctcatg gatgccacat accagcgcag atagccgttc tcacttaaca
     4981 gcagaccttt acctgcaaag cgggaaagga cagccgggat gttcatcatt gagttcagcg
     5041 gatcgcgttt cagcaaccag gcaataccca ggaacggctt gacgaacact ttgtcataca
     5101 gccagtcaaa tccccaggcg ttgtaccacc aggtgcccag cagacggccc ggcgcactgt
     5161 tggcgatgga ggtcaccaga gtacgtttac ccagccacag ccaggctgcc agcagaatgc
     5221 cgaccaccgc gaccacgcca gaggtaattt ccagggtcaa catgctgccg tgcgccagtt
     5281 ccgtcgtttg cggaagcacg ccctgcagcg gcggtacaat cagtgcgcca acgaaggtgg
     5341 aaaggatcag cagcacaatc agcggcaggc tgtgagttac ccctttcacg gcgtgagcgt
     5401 gaatttgttc ttttccgtgg aagacgatga aaatcatacg gaaggtgtag agcgaggtca
     5461 taaacgcacc gaccagacct gccaccatca gattgatatg accattcgcc atcgcacccg
     5521 cgaggatctc atccttactg aagaagcccg cagtgaccag cggtagtgcc gacagtgctg
     5581 cgccgcccac caggaagcag agataaacca gcggaataga tttacgcaga ccgcccatct
     5641 tgaagatgtt ctgttcgtga tggcaggcca gaatgacgga accggatgcc aggaacagca
     5701 gcgctttaaa gaacgcgtga gtcatcaagt ggaaaatcgc cgcatcccat gcctgcacgc
     5761 caagcgcgag gaacatgtag ccaatctggc tcatggtaga gtaagcgaga acacgtttga
     5821 tgtcggtctg taccagcgcg gcaaaaccgg ccagcagcag cgtaaccgcc ccgacaatac
     5881 ccaccagatg cagaacttcc ggcgtcacca ggaacaggcc gtgggtacgg gcgatcaggt
     5941 agacacccgc ggttaccatc gttgcggcgt ggatcagcgc ggagacaggc gtcgggcccg
     6001 ccatcgcgtc agcaagccat gtctgcaacg gcaactgcgc agatttaccg accgcaccgc
     6061 ccagcagcat cagcgtcgcc cacatcagca tgttattgcc gtcagcaaag tgcgctggtg
     6121 ccagttccac catttcgcgg aagttcaggg tgcccagttc gttgtaaaga atgaacagtg
     6181 cgaaagcgag gaaaacgtca cccacacggg tcacgacgaa cgctttcatt gccgctgcgc
     6241 cattcttcgg atcggtgtaa tagaaaccga tcagcagata ggagcacagg cccacgcctt
     6301 cccagccgag gtacatcagc agcaggttat cggcgagaac cagaactacc atgctggcga
     6361 tgaacaggtt ggtgtaggcg aagaagcgag agtagccctc ttcaccgcgc atgtaccagg
     6421 aggcgtacat gtgaataagg aaacccacac cagtgaccac cgagagcatg gtcagcgaca
     6481 ggccgtccag caccaggtta aaaccgatgt taaagtcgcc taccgacatc cacgtccaca
     6541 gcggctggct gtatgactgc tcgccgttag cgaagaaatc aacgccgata aaggcggtta
     6601 ccagcgccgc caggcccaca gagccaacgc cgacgatcgc cgaaacgttt tcagaccagc
     6661 gcccacggga gaacgccagc aggacgaagc caatcaatgg caaaataatg gttaaggcaa
     6721 gcatgttcat ccgcgcatct cacttactga atcgatgttc aggttctggc gacgacggtg
     6781 aagttgcagc agcagcgcaa ggccgatact cgcttctgcc gccgcgaggc tgatggcgag
     6841 aatgtacatc acctgaccgt cggtctgacc ccagtagctt ccggcgacca caaaggccag
     6901 cgcggaggcg ttaatcatga tttccagacc aatcagcata aacagcagat tgcgacggat
     6961 aaccagaccg gttaagccaa gaacgaataa gattgccgcg aggatcagtc catgttgtaa
     7021 ggggatcatg cgtgctcctc cgtttttctt ttcgcgctgt cgtctttacg attgctcagc
     7081 acttcacccg cacgctcttc acgaccgacg tggaaggcca caaccagacc cgcgagcagc
     7141 agcatagaag ccagttccac cgccagtacg taaggcccga acagcgtaat acccactgct
     7201 ttagcactga ttggcgtacc gtcgataccc tgatcattaa caccaaggat ggcgtaaaca
     7261 atcaccacca gcatgatggc cgacaaaatt gccggaccaa tccacacctg cggtttcagc
     7321 cactggcgtt cctgttcgat ttctgaaccg cccaggttga gcatcatcac cacgaacacg
     7381 aacagcacca taatggcacc cgcgtagacg ataatttcca gcgcaccggc gaagtaagcg
     7441 cccagtgaga agaacacccc ggaaatcgcc agcagcgaaa taatcaggta cagcagtgcg
     7501 tgtaccggat tggtatgggt gatcactcgc aaggtcgcaa gtatggctat caggccacag
     7561 atataaaaag cgaactccat tgcccctctc cttacggtaa caggctcttg acgtcgatag
     7621 gcttggcttc gttctctgct tcgcccttat ctttgccgtc gattgccata cctgccatcc
     7681 ggtagaagtt atattccggg tatttgcccg gaccggagat cagcagatcc tctttttcgt
     7741 aaaccagatc ctggcgcttg tattccccca tttcgaaatc cggggttaac tgaatcgccg
     7801 tggtcggaca ggcttcttcg cacagaccac agaaaatgca gcgtgagaag ttgatgcgga
     7861 aaaattccgg gtaccagcga ccgtctttgg tttctgcttt ttgcagcgag atacagccga
     7921 ccgggcaggc taccgcgcag aggttacagg ctacgcaacg ctcttcgccg tccgggtcgc
     7981 gggtcagaac gatacgacca cgataacggg gcggcagata gaccggctct tccgggtaca
     8041 ttcgcgtttc gcgtttggcg aacgcgtgca ggccgatcat ccagatacta cgaacctggg
     8101 tgccgaaacc tactaacaat tcttttaagg tcatggtctt attgcccctt attgcgcctg
     8161 ccagagaatg acagccgccg ttaccagcaa gttgatcagc gtcagcggca ggcagatttt
     8221 ccagccgaag gacattacct ggtcataacg cggacgcggt aacgacgcac gaatcaaaat
     8281 gaacatcatc ataaagaacg cggttttcag cgcgaaccag atgaatggcg gtaacaacgg
     8341 gccttgccag ccaccgaaga acagcgtcac catcaatgca gagatggtca caatcccgat
     8401 gtactcaccc acgaagaaca gaccgaactt cataccggaa tattcaatgt ggtaaccatc
     8461 cgccagttcc tgctcggctt ccggctggtc aaacgggtga cggtgacata ccgccacgcc
     8521 cgcgatggca aaggtaataa aaccaaagaa ttgcgggata acgttccaca catgcgcctg
     8581 gctgttgacg atgtcggtca tgttgaatga accggcctgc gccaccacgc ccatcaggga
     8641 aagcccgagg aacacttcgt agctcagggt ctgcgcagaa gcacgcatcg cacccagcaa
     8701 cgagtatttg ttgttacttg accagcccgc aaacagcacc gcgtaaaccg ccagacctgc
     8761 catcatcagg aagaacaaaa tcccgatgtt caggtcggca accacccaac ccggactgac
     8821 tggcacaatc gcaaaggcca gcagcagcga ggtaaaggca atcatcggtg ccagggtaaa
     8881 gatgacgcga tccgagaatt tcgggatcca gtcttcttta aagaacattt tgatcatgtc
     8941 cgcaaccagc tggagcgaac cgccccagcc aacacggtta ggtccgtaac ggttctggaa
     9001 cagacccagc aggcgacgtt cgccaaagct catgaatgcc ccgcaggtga caaccaccag
     9061 caggatcacc accgctttga ggatggtcag caggatctca atcagttccg gtgatatcca
     9121 actcattgtt gtgcctcctt gagatcctca agatgcgcgc cagccagcac cggagcaatg
     9181 ccggacatac ccatcggcaa acccacctgc cctgccgtca gcccttcagc gatttcaacc
     9241 ggcagcgtga ccgtgttgcc atcgtaacta aaggagacgc gagcgcctgc gttcacgccc
     9301 aacttcgcgg catccgctgg gttgagtttg atgtacggct gcggcatacg gctctggaag
     9361 accggagcac gctgtgacaa ttcatcgctg ccaaacaggt ggtaatacgg cgcgatacgc
     9421 catttcccgt cctgcggctg gaagcgtgcc ggtacgctgg tgaagtaatc cagaccattt
     9481 tcgctggttt caaacagacg cacgcccgga tcgccaaagc gcagtttgcc gcccacttcg
     9541 tcctggaatt tgttccacgc ctgcggggag ttccagcccg gcgcccaggc aaacggcact
     9601 tgcgaacggt gcgcagtcgg ctggttgtta ccttccatcg agaaggtgaa catggtgtca
     9661 atatcctgcg gctgacgcgg ctcatgaacg ctgatattgg cgcgcatggc ggtacgaccg
     9721 ctgtaacggt gcggttcacg ggccagtttc tgcccacgaa tacggaatgt cgcatccggc
     9781 gcagcatctt tgatacctgc cagttccggg attttcgcca caacagcgtc aatcacatgg
     9841 tcgagctgcg tccagtccac ttcacggctc agcagggtgc tgtgcagcga gtgtaaccag
     9901 cgccagcttt ccagcatgac agttttgctg tcgtaataag caggatcgta aacctggaag
     9961 aaacgttggg cgcggccttc gttgttgatc accgtaccgt cgctttcagc aaagctggca
    10021 gcagaaagta ccagatgggc gttttccata atcgctgtgc gttgatgatc aaccaccatc
    10081 accagcggcg ctttagccag cgcagcattc acgcgggtag cggaagcgtg acgatgcaga
    10141 tcgttttcca gcaccaccac cgcgtcggcg cgtccggttt ccagttcggt caacgcttct
    10201 tcaagcgaac cgccacccat aatgcccagc cccatgctgt tgacggaacg ggcaatcatg
    10261 gtgataccaa cgtcagcgcc gcgacctttc agggctttcg cgacgttagc cgcagcctga
    10321 atcacctcta tgctaccggc gttcgtcccg gagataatca acggtttctt cgcacctgcc
    10381 agtgcctgca cgatgacgtc gattttgctt tgcagctcag gttcgatacc gtcaaccgct
    10441 ggcgcagagt tatccagcgc atgggcgatg gcaaaaccta aacgcgcctg atcttcaacc
    10501 ggtgcgcggt aagtccacgc cgcgatatca tccagacggg tgtcatcaac gttggtaaca
    10561 aacagcggat gcttcgcacg ttgaccgatg ttgaggattg ccgcaatctg ccagtcagcc
    10621 actttctgtg ctgccgccat ttcgcgcgct ttacctttca cagcctgacg cactgccagc
    10681 gcgacgcgcg cgccggtctg ggtaacgtct tcgcccagca ccagtaccgc atcgtaagat
    10741 tcgatttcgc gcagagccgg agtataaatg ccgccttcac gcagcacttt cagcgccagt
    10801 tgcagacgtt cctgctcacc gtgagcgata ccggtgtaga agttttcttc gcccaccagt
    10861 tcacgcagcg caaagttgct ttccacgctg gcacgcggag aaccaatacc gatcactttc
    10921 ttcgactgac gcagaatatc tgccgcgccc tgcattgcct gttcggcgtt gagggtaatg
    10981 aaatcatcgc cacgacgctg tactggctga cgcggacgat ccttcaggtt gacgtaaccg
    11041 taaccgaaac gaccacggtc gcagaggaag tagtggttta ccgtaccgtt gtaacggttt
    11101 tcgatacgac gcagttcgcc gtaacgttca ccggggctga tgttacagcc gatggaacat
    11161 tgctggcaga tgctcggcgc aaactgcata tcccatttac ggttgtaacg ctcggagtgc
    11221 gttttgtcgg taaatacgcc ggtcgggcaa atttcgacca ggttaccgga aaattcgctt
    11281 tccagcgtgc cgtcttccgg gcgaccgaag tagacgttgt cgtgcgcacc gtaaacgccc
    11341 agatctgtac cgtcagcgta atctttgtag taacgcacac agcggtagca ggcgatgcag
    11401 cggttcattt cgtgagagat gaatggcccc aaatcctgat tacggtgggt acgtttggtg
    11461 aaacggtagc gacggaagct gtgtccggtc atcacggtca tatcctgaag atggcagtta
    11521 ccgccttctt cacataccgg acagtcgtgc gggtggttgg tcattaacca ctcgaccaca
    11581 ctttcacgga actgtttcgc ttcttcgtcg tcaatggaaa taaaggtgcc atcggaagcc
    11641 ggtgtcatac aggacatcac caggcgacca cgcgtgtctt ccgcgttttg gtattgcttc
    11701 accgcacact ggcggcaagc accgacgctt cccagcgccg gatgccagca aaagtaagga
    11761 atatcaaggc ccagagacag acaagcttcc agcaggttgt ccgctccgtt gacctcgtat
    11821 tctttgccgt ctacatgaat tgtagccatt agcatgcttc cagttttctc agtcagagac
    11881 tgagcgttaa tcgaaattcg gttaccagcg ctctttcagc aggttcggct gaatcccatt
    11941 aatcaaatgg gtattgctga acggctgttt gattcccgcc tcaaattctt cgcggaaata
    12001 tttgatggcg ctctgtaacg gctccactgc accaggtgcg tgggcacaga aagttttacc
    12061 cgggcctaag aatcgacaca gttgctcaag tgtttcgata tcgcccggct gaccttcacc
    12121 acgctccagc gcacgcagaa ttttcacgct ccacggcaga ccgtcgcggc acggcgtaca
    12181 ccagccgcag gattcacggg cgaaaaactc ttccaggtta cgcaccagcg acaccatgtt
    12241 gatctcatgg tcaaccgcca tcgccagcgc cgtacccaga cggcttcctg ctttaccgat
    12301 actttcgaat tccatcggca ggtcaagatg cgcttcggtc aggaagtcgg tgcccgcgcc
    12361 gcctggctgc caggctttga atttcagacc gtcacgcata ccaccggcgt aatcttcgag
    12421 gatctcgcgc gcggtagtac caaacggcag ttcccacagg cccggatttt tcacccgacc
    12481 ggagaagccc atcagcttgg tgccagcatc tttacttttc gagatgttct gataccactc
    12541 cacgccgtta gcgaggatcg ccggaacgtt acacagggtt tcgacgttgt tgacacaggt
    12601 cggtttaccc catgcgccgg aggtcgccgg gaatggaggc ttcgagcgtg ggttagcacg
    12661 acgtccttcc agggagttga ttaacgctgt ttcttccccg cagatgtagc gccctgcccc
    12721 ggtatggacg aacagttcga agtcaaaacc tgttcccata atatttttgc caagcagacc
    12781 tgcttcggtg gcttcggcaa tggcacggcg tagattcact gccgcttcga tatattcgcc
    12841 acgcaggaag atgtagccac ggtaagcttt cagcgcaaac gcggagatga gcataccttc
    12901 caccagcagg tgcggcagtt gctccatcaa caggcggtct ttataggtgc ccggctccat
    12961 ttcatcggcg ttacacagca ggtaacggat gttcatggat tcgtctttcg gcatcaggct
    13021 ccacttcagg ccagtcgaaa agcccgcgcc gccgcgccct ttcagaccag cgtcttttac
    13081 ctgattaacg atttcgtccg gagacagccc ggtcagcgcc ttacgcgccc cttcgtaacc
    13141 atttttgctg cggtattcgt ccagccacac tggctgtttg tcatcgcgca gacgccaggt
    13201 cagcggatgc gtttcgggag tacggataat gtttttcatt tataccgctc cagcagttca
    13261 gggatcgctt ccggggtcag atgcgcgtga gtgtcctcat cgatcatcat atttggccct
    13321 ttatcacagt tccccaggca gcaggttggc agcagcgtaa agcgaccgtc aaacgtcgtt
    13381 tgccctggtt tgatgttcag ttttttctcc agcgccgcct gaataccctg gtaaccgttg
    13441 atgtgacaga ccacgctgtc gcaataacgg atcacatggc gaccaaccgg ttggcggaag
    13501 atctggctat agaacgttgc cacgccttcg acatcgctcg ccggaatacc cagcacatcg
    13561 gcgatcgcgt ggatcgcgcc gtccggcacc cagccacgct gcttctgaac gattttcagc
    13621 gcttcaatgg acgccgcacg cgggtcttcg tagtggtgca tctcgtgctc aatcgcttca
    13681 cgctctgccg cactcagctc aaaagcctcg gtttgtggtt gttgattctc gtgcataatt
    13741 agcggtccac atctgacata acaaaatcga tactgcccag ataaacaatc aggtcagaca
    13801 ccaggctgcc gcggatcgcc gccggaattt gctgcaaatg cgcataactc ggcgtgcgga
    13861 tacgagtgcg ataactcatg gtgctaccgt cgctggtcag gtagtaactg ttgatcccct
    13921 tggtcgcctc aatcatctgg aaagattcat tggcaggcat caccggcccc cacgacactt
    13981 gcaggaagtg ggtgatcagg gtttcgatat gttgcagcgt gcgctctttc ggcggcggcg
    14041 tggtcagcgg gtgatccgct ttgaacgggc cttccggcat gttgttgagg cactgctcaa
    14101 gaatgcgcag actctggcgc agctcttcca ctttcagcat tacgcgggtg tagcagtcag
    14161 aaacaccacc accaaccggg atttcaaagt cgaagttttc atagccagaa taaggacgcg
    14221 ccttacgcac gtcgaagtcg atcccggtag cacgcaggcc cgcgccagtg gtgccccact
    14281 ccagcgcctc tttcgcgcca taggcggcaa cgccctggga acgacctttc agaatggtat
    14341 tttgcagcgc cgctttctcg taagacgcca gacgtttcgg catccagtcg aggaactcac
    14401 gcagcaggcg atcccagccg cgcggcaggt cgtgcgctac gccgccaata cggaaccacg
    14461 ccgggtgcat acggaaaccc gtgatcgctt ccaccagatc gtaaattttc tgacgatcgg
    14521 taaaggcgaa gaacacgggc gtcattgcgc cgacgtcctg aataaaggtc gagatgtaca
    14581 gcaggtggct gttgatacgg aacagttcgg agagcataac gcgaatgacg ttaacgcgat
    14641 ccggcacggt gatcccggcc agtttctcta ccgccagcac gtaaggcatt tcgttaacgc
    14701 agccgccgag gtattcaata cggtcggtat acggaatgta gctgtgccag gactggcgct
    14761 cgcccatttt ctccgcacca cggtggtggt agccgatgtc tggtacgcag tcgacaatct
    14821 cttcgccgtc gagttgtaaa acgatacgga aagccccgtg cgccgacggg tggttcggac
    14881 cgaggttgag gaacatgaag tcctcgtttt cggtgccgcg cttcatcccc cactcttccg
    14941 gtttgaaggt cagggcttcc atctccagat cctgtttggc tttggtcagc tcaaacggcg
    15001 agaattcggt agcgcgcgcc gggtaatctt tacgcagcgg gtgacctttc caggtttgcg
    15061 gcatcatgat gcggcgcagg ttcgggtgac cgtcgaaagt aatgccaaac agatcccagg
    15121 tttcacgctc ataccagtta gcgttcggga acagtttggt gaaggtcggt acgtgcaggt
    15181 cgttttctgc cagcgccacc ttcagcatga tgtcgcggtt acgatcgata gaaatcagat
    15241 ggtagaaaac ggaaaaatcc gcggcaggta acccttcgcg gtgtgtgcgc agacgttcgt
    15301 ccatgccgtg taagtcaaac agcatgacgt aaggtttcgg cagtttcttt aagaaatcgc
    15361 caacttccag taattgttca cgcttgatcc acacaacggg aaccccggtg cgagtcgcct
    15421 gaacagtaaa ggcatccggc ccaaaacggt tgcgcagttc gccaattacc ggatcatcaa
    15481 gatgatcgcg ggtctgccag gcgggttctt gcgcggttaa gtcggtcata ttgttcacca
    15541 ttgcaaatgg tccgtggtga ctgtcggcgc agtaagcttc gcgaaataga tttaggaata
    15601 agcgaagaaa aatccctttg ccgacaggcg caaattaaat ctcgtcaggt gtacgcaggt
    15661 tggttacggc aatacgttca ccgcgcttgc gttcgcgctc tgattgcata ttggcgcgat
    15721 aaacgccctg atcgccaacc acccaggaga gcggacgacg ttctttgccg atagattcct
    15781 gcaacagcat cagtgcctgc atgtacgctt caggacgcgg cgggcagccc gggatataca
    15841 catcaaccgg gatgaattta tcgacgccct gcacaacgga ataaatatcg tacataccac
    15901 cagagttggc acaggcaccc attgagataa cccattttgg ttccagcatc tggtcataca
    15961 gacgctgaat aaccggtgcc attttggtaa agcaggttcc tgcaaccacc atcaggtcag
    16021 cctgacgcgg cgaagcacgc aatacttctg cgccaaaacg cgccacgtca tgcaccgcgg
    16081 taaacgaagt caccatctca acgtaacagc aggaaagacc gaagttatac ggccaaattg
    16141 agtttttacg accccagtta accatgtcat tgagcttgcc cataaacacg tttttgttaa
    16201 cttcttgctc cagagggtcg gttacgatct cctgcttttg cagggggtaa cggtcgttct
    16261 caccgttggg atctatgcgg gtgagcgtat aatccatctt aatgcctcgc ggttagcgtt
    16321 gacgattagc gatactgttc gtttccgggt tcatacgctc gcggcgtgaa cgcgcgggcg
    16381 tccagtccag cgcgccaata cgcaccagat aaaccagacc tgccagtaac acaaaaataa
    16441 aaattgcagc ttccacaaag cctacccagc cgctttcgcg gatagaggtt gaccatgcga
    16501 acagatacag cgcttcaacg tcgaagataa cgaagaacat ggccaccaga taaaacttgg
    16561 cggacaggcg taagcgggcg gagccgaccg agtcgatacc ggattcaaac ggcacgtttt
    16621 tcgacctcgc gcgtgcgcga ccgcctaaaa accaaccgcc taccagcatc aggcaacaca
    16681 ggccaatggc aacgataaga aagatagcga atgcccagtg atgagcgatg acttcagtgg
    16741 atgttgacat actcattgct tactcatcaa aagtagcgcc agattcactg ctcttcacgg
    16801 cagatggacg ccacatcgat tcatggggag gaataaaaaa aaccttacaa tcactgt
//
LOCUS       DAZFWL020000098        16837 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFWL020000098 DAZFWL020000000
VERSION     DAZFWL020000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.098"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(87..2048)
                     /gene="topB"
                     /locus_tag="ACHKNP_RS17190"
                     /old_locus_tag="pgaptmp_003438"
     CDS             complement(87..2048)
                     /gene="topB"
                     /locus_tag="ACHKNP_RS17190"
                     /old_locus_tag="pgaptmp_003438"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGU4705548.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(2053..3096)
                     /gene="selD"
                     /locus_tag="ACHKNP_RS17195"
                     /old_locus_tag="pgaptmp_003439"
     CDS             complement(2053..3096)
                     /gene="selD"
                     /locus_tag="ACHKNP_RS17195"
                     /old_locus_tag="pgaptmp_003439"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HGU4705549.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIH"
     gene            complement(3213..3764)
                     /locus_tag="ACHKNP_RS17200"
                     /old_locus_tag="pgaptmp_003440"
     CDS             complement(3213..3764)
                     /locus_tag="ACHKNP_RS17200"
                     /old_locus_tag="pgaptmp_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HGU4705550.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            3925..5781
                     /gene="sppA"
                     /locus_tag="ACHKNP_RS17205"
                     /old_locus_tag="pgaptmp_003441"
     CDS             3925..5781
                     /gene="sppA"
                     /locus_tag="ACHKNP_RS17205"
                     /old_locus_tag="pgaptmp_003441"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HGU4705551.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            5948..6964
                     /gene="ansA"
                     /locus_tag="ACHKNP_RS17210"
                     /old_locus_tag="pgaptmp_003442"
     CDS             5948..6964
                     /gene="ansA"
                     /locus_tag="ACHKNP_RS17210"
                     /old_locus_tag="pgaptmp_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGU4705552.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            6975..7616
                     /gene="pncA"
                     /locus_tag="ACHKNP_RS17215"
                     /old_locus_tag="pgaptmp_003443"
     CDS             6975..7616
                     /gene="pncA"
                     /locus_tag="ACHKNP_RS17215"
                     /old_locus_tag="pgaptmp_003443"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HGU4705553.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7777..8049)
                     /locus_tag="ACHKNP_RS17220"
                     /old_locus_tag="pgaptmp_003444"
     CDS             complement(7777..8049)
                     /locus_tag="ACHKNP_RS17220"
                     /old_locus_tag="pgaptmp_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HGU4705554.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8091..8504)
                     /gene="msrB"
                     /locus_tag="ACHKNP_RS17225"
                     /old_locus_tag="pgaptmp_003445"
     CDS             complement(8091..8504)
                     /gene="msrB"
                     /locus_tag="ACHKNP_RS17225"
                     /old_locus_tag="pgaptmp_003445"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGU4705555.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8846..9841
                     /gene="gapA"
                     /locus_tag="ACHKNP_RS17230"
                     /old_locus_tag="pgaptmp_003446"
     CDS             8846..9841
                     /gene="gapA"
                     /locus_tag="ACHKNP_RS17230"
                     /old_locus_tag="pgaptmp_003446"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU4705556.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9925..10809
                     /locus_tag="ACHKNP_RS17235"
                     /old_locus_tag="pgaptmp_003447"
     CDS             9925..10809
                     /locus_tag="ACHKNP_RS17235"
                     /old_locus_tag="pgaptmp_003447"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HGU4705557.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(10860..11714)
                     /gene="yeaE"
                     /locus_tag="ACHKNP_RS17240"
                     /old_locus_tag="pgaptmp_003448"
     CDS             complement(10860..11714)
                     /gene="yeaE"
                     /locus_tag="ACHKNP_RS17240"
                     /old_locus_tag="pgaptmp_003448"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HGU4705558.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(11804..12550)
                     /gene="mipA"
                     /locus_tag="ACHKNP_RS17245"
                     /old_locus_tag="pgaptmp_003449"
     CDS             complement(11804..12550)
                     /gene="mipA"
                     /locus_tag="ACHKNP_RS17245"
                     /old_locus_tag="pgaptmp_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HGU4705559.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            12986..14920
                     /gene="yeaG"
                     /locus_tag="ACHKNP_RS17250"
                     /old_locus_tag="pgaptmp_003450"
     CDS             12986..14920
                     /gene="yeaG"
                     /locus_tag="ACHKNP_RS17250"
                     /old_locus_tag="pgaptmp_003450"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HGU4705560.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            15033..16316
                     /locus_tag="ACHKNP_RS17255"
                     /old_locus_tag="pgaptmp_003451"
     CDS             15033..16316
                     /locus_tag="ACHKNP_RS17255"
                     /old_locus_tag="pgaptmp_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HGU4705561.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            16463..>16837
                     /locus_tag="ACHKNP_RS17260"
                     /old_locus_tag="pgaptmp_003452"
     CDS             16463..>16837
                     /locus_tag="ACHKNP_RS17260"
                     /old_locus_tag="pgaptmp_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU4705562.1"
                     /translation="MIQSTRISMGLFFKYCLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILS"
BASE COUNT         4170 a         4333 c         4112 g         4222 t
ORIGIN      
        1 tccatgcaaa aaaaatatcc aacgaatttt cttgatccgc tggatatttc agaatatgac
       61 tcgaatagca cgaaagattc actcgcttac gctatcgccc cgcttccgac ttcatctgct
      121 ggcggacttt ttttcgcact acgtttacgc ggtgcagcct ttttcttatc agcactgcca
      181 ccactgcccg gagccacaat gccgcgaaac tgccgcaccg gcgtacgttt ggcttgatca
      241 ataagctgat atagcgtccc caccagcggc tgcataaagt cctgatagcg acactgcttt
      301 tcgctgattt gcgtcagcac cgattcccag tgcgcggtca tgtccggtcg cgtcgccatt
      361 tctggcagcg aatggaataa cgcttttccg gcgtcggtag agtggatata gcgccctttt
      421 ttggtcagaa aaccacgctt gaacagcagt tcaataatcc cggcacgggt tgcttctgtc
      481 cccagaccat cggtcgcacg aaggatcttt ttcagatctt tgtcctgcac aaagcgcgcg
      541 atcccggtca tcgccgaaag cagtgttgca tcggtaaaat ggcgcggcgg ctgggtttgc
      601 cgctctacca cttcaccttt ttcacacagc aactcatcgc ctttcgccac cacaggcaat
      661 ggcgtaccgt cgttttcttc atcgcgctct ttgctgccta acagcgtgcg ccagcctgct
      721 tcagcaagaa aacgggcttt agcgacaaat ttgcctttgg caatgtccag ttcgataaca
      781 cacttgcgga acaccgcatc cgggcagaac tgcatcaggt attgacgggc aatcaggtta
      841 tagaccttcg cttcgttctc cgtcaggttg atcgcagaac tccgtgcggt cgggatgatg
      901 gcgtggtgcg catcgacctt tttgtcatcc caacagcggt tgcgtatatc tggatctacc
      961 actgactgcg gcaacagatc cggtgcatga acgctgatgg cattcatcac cgcgtggcgt
     1021 ccggcaaaat gttcttctgg caaatagcga caatcggaac gcggataagt gattagcttg
     1081 tgcgtttcgt acagcttctg gcagatatca agcacgttct gcgcgctcag accgaagcgt
     1141 tttgccgctt caatctgtaa cgccgaaagc gaaaacggca gcggcgcgga ttctgattcc
     1201 cgtttatcgt tatagctggt gacaatagcc ggttgaccac taatgcggtt aaccacatgc
     1261 tccgccagtg gacgatgtaa caagcgccct tcttcatcct ggtacggttc acacgcttcg
     1321 ctcggttgcc agatagcggt aaaccgctca tcggcaggtg tcacgatatg cgctttgact
     1381 tcaaagaagt ctttcgccac gaagttttca atctcttcat cgcggcgcac caccagcccg
     1441 agcacgggtg tctgcacgcg tcccacggaa agtacgccct gataaccggc attgcgaccg
     1501 agaatggtat acgcacgggt catattgatg ccgtacagcc agtcggcacg cgctcgcgcc
     1561 agcgcagaaa cgcacagcgg tacaaactca ctgttggaac gaagacggtc gatcgcccgc
     1621 tcaaccgcct gcgggttcag gtcgtttatc aagcaacgct gcacctgctg gcgcttttcc
     1681 ggtgccagtt gcagatagtc cagcacttca tccaccagca attgcccttc acgatccggg
     1741 tccccggcgt gaacgatttc gctggcttca tgcaggaagc gtttgatgac gttaagttgt
     1801 ttggtcacgg agggtcgggg ctgtaattgc cacttttccg ggacaatcgg caaatccgca
     1861 agattccagc gcgcatagcg gctgtcgtag gcgtctggct gcgcctgctc aagcaggtga
     1921 ccgatacacc aggtcaccac ctgaccatta ccgcactcga taaagccatc gcctttacgg
     1981 tgcggtttgg gcaggacatc agcaatggcg cgcgccagac tcggtttttc ggcaataaac
     2041 aaccgcattg aattaatgga tctcaaccat ggcacgaccg ccgcgcgctg gcaccagttc
     2101 gccaatcgcc gtcagttcaa tgccaaactc ggcggctgta gttttaacct cattttctgc
     2161 ttccggcatg accgccagca gcaaaccgcc agaagtttgc ggatcgcaca gcagatcgcg
     2221 cacttcacgc ggcatttcac ccatcagatg accgtagctg gcaaagttac gttcggtgcc
     2281 gccaggcact gcgcccaact taatgtactc ttcaaccccc ggcaattttg ggatcgcgtc
     2341 atagtcaacg cgcgcctgca cacctgcgcc ctgacacatt tcgctcaaat ggcccagcag
     2401 accaaaacca gtaacgtcgg tcatcgcttt tacgccttcg atgttggcaa aggacgcacc
     2461 tgcgatgttc atccggcaca tcacttccgt cgccagtccc tgatgttctg gtttcaacag
     2521 tgattttttc tcagccgtgg taagaacgcc gatccccagc ggtttcgtca ggaacagttt
     2581 gcatccggct tgtgcggtac tgtttttctt cacccgctcg gtcggtacga tccccgttac
     2641 cgccagacca aaaatcggct ccggcgcatc gatggaatga ccgccagcca gcgcaatacc
     2701 cgcctgacgg catgcatagc gtccaccttc ggttacttcg cgggcaattt ctggggaaag
     2761 tttgttaatc ggccagccga ggatcgcaat cgccataatc ggtttgcccc ccatcgcgaa
     2821 gatatcgctg atggcgttag tcgccgcaat gcggccaaaa tcgaaaggat tatcaacgat
     2881 cggcataaag aagtcggtgg tactgataac gctggtgcca ttgcccagat cgtacaccgc
     2941 cgcatcgtcg cgggtctcat tacccacaag caaattcgga tcaacaaact tcgcctgctc
     3001 actgtgcagg atggtttcca acacttttgg ggaaatttta cagccgcaac cagctccgtg
     3061 gctgtattgg gtcaaacgaa tcgagttctc gctcatggac atctcctgtc aatgcaatcc
     3121 gggtatggta accctcattc cgtgaagtga taagtgagag tgtctgaatt cctgcgcctt
     3181 tgctcacaat ccagacagtt tcgcgacaat tatcagaaat aagttacaaa cggcgtcggg
     3241 tccgggacgt taatcgacgt agatgctttc aactgcggcg tgccgaggta gagaaaaccg
     3301 acaattttat cctgctcacg gcaaccgaat gcttcacgca ctaccggact ttcagttaat
     3361 gcgccactgc gccagatgcc gccaaatcct tgggcaacgg ctgccatttg catcgccatg
     3421 accgcgcatc cggcagacat ttcctgttcc catcgcggga ctttatgatt ttcttcgcat
     3481 ttcgccacca ccgtgatgat gagcggtgcg cggaacggcg cattacgggc tttgtcgata
     3541 gctttgtcat cactaccggc agcaatcgcc ccctgttcca gtacggcgct gaaacgctcg
     3601 cgcccttccc cttcaatcac aaaaaaatgc cacggttgca tggacttatg gtccggcgca
     3661 cgcatacccg cacgcaggat gttttgcagt tgttcacccg ttggcgcggg ttcagccagg
     3721 cgggaggcgc tacggcgatt gatcaatagt tcgagtgcat ccatttgatt aactcctgtc
     3781 gtgatattta ttcacaaaat taacacgaga gtggattttg ttacagcaca gtccgcaatt
     3841 cctgctgaca agtgccggtt gggtcattac gataaccaca tctattgcgc ctgtgacagg
     3901 tgtgacctta agttgggaga atacatgcga accctttggc gatttattgc cggatttttt
     3961 aaatggacgt ggcgtctgct gaatttcgtc cgtgaaatgg tacttaacct gttctttatt
     4021 ttcctcgtac tggttggtgt ggggatctgg atgcaggtca gtggtggtga ttcgaaagaa
     4081 acggccagtc gtggcgcact gctgctggac atttctggtg tgatcgtcga taaacccgac
     4141 agttctcagc ggtttagtaa attaagccgc cagctgcttg gtgccagttc cgatcgtctg
     4201 caggaaaact cactgtttga tatcgtcaac accattcgcc aggcgaagga cgaccgcaat
     4261 atcaccggta tcgtgatgga tctgaaaaac ttcgcaggcg gcgaccagcc gtctatgcag
     4321 tacatcggca aagccctgaa agagtttcgt gacagcggga aaccggttta tgccgttggc
     4381 gagaactaca gccaggggca atattatctc gccagtttcg ccaataaaat ttggctgtca
     4441 ccgcaaggcg tggttgatct gcacggtttt gctaccaacg gtctgtacta caaatcgttg
     4501 ctggataagc tgaaagtttc cacccatgtg ttccgcgtgg gtacgtataa atctgccgtt
     4561 gaaccgttta ttcgtgatga tatgtcaccg gcagcccgcg aagctgacag ccgctggatt
     4621 ggtgagctgt ggcaaaacta tctgaatact gttgccgcta accggcagat ccctgctcag
     4681 caggtattcc ctggcgcgca agggttgctt gagggtttaa ccaaaaccgg tggcgatacc
     4741 gcgaaatatg cactggaaaa caagctggtc gatgcactgg catcgagtgc cgaaatcgaa
     4801 aaagctctga ccaaagagtt cggctggagt aagactgata aaaattatcg cgccatcagt
     4861 tattacgatt acgcattgaa aacgccggca gataccggtg acagcatcgg tgtcgtcttc
     4921 gctaatggcg caattatgga tggcgaggaa actcagggga atgttggcgg tgataccact
     4981 gcggcacaaa tccgcgacgc tcgccttgac ccgaaagtga aagcgattgt cctgcgtgtt
     5041 aatagcccag gcggcagcgt taccgcgtct gaagtgattc gcgctgaact ggcagcagcc
     5101 cgggcagcgg gtaagcctgt ggttgtatcg atgggcggca tggcggcatc tggtggttac
     5161 tggatttcca cgccagctaa ttacattgtg gctaacccca gcaccctgac cggttctatc
     5221 ggtatcttcg gcgtgatcac caccgtagaa aatagtctgg attcgattgg tgttcatact
     5281 gatggtgtct caacttcacc gctggcggat gtttctatca ccagggcact gccgccggaa
     5341 gcgcagcaga tgatgcaatt aagcattgag aatggctata aacgctttat cacgctggtt
     5401 gctgatgcgc gtcattcgac gccggagcaa attgataaaa tcgcccaggg ccacgtctgg
     5461 accggtcagg atgcaaaagc taacgggctg gtcgatagtc tcggggattt cgatgatgcg
     5521 gttgccaaag cagcagagct ggcaaaagtg aaacagtggc atctggaata ctacgttgat
     5581 gaaccgacct tcttcgacaa agtgatggac aacatgtctg gttctgtccg ggcaatgttg
     5641 ccagatgcgt tccaggccat gttacctgca ccgcttgcct cggtagcctc taccgttaaa
     5701 agtgaaagcg acaagctggc cgcgtttaac gacccacaaa accgttatgc gttttgcctg
     5761 acctgcgcca acgtgcgtta agtcttgtac tgagtggccg acagatcgtc ggccacatta
     5821 ttttttacct cgacgaatcc tcttcccgct gtctcgaccc atatttcctt atccgcatag
     5881 tatcaggtgc gctcctcctg cctcacgtat atacttttgc tctttcgata tcattcatat
     5941 caatatcatg caaaagaaat caatttacgt cgcctacacg ggcgggacca tcgggatgca
     6001 gcgttccgag cagggttata ttccggtgtc aggtcatcta caacgccaac tggcgctgat
     6061 gccggaattc catcgcccgg agatgccaga tttcaccatt catgaatata cgccgttgat
     6121 ggattcttct gatatgacgc cggaagactg gcagcatatt gctgaagata ttaaagcgca
     6181 ctatgacgac tatgatggtt ttgtcattct acacggtacc gacacgatgg cgtataccgc
     6241 ctctgcgctg tcgttcatgc tcgagaatct cggtaaaccg gtcattgtga cagggtcaca
     6301 aatcccgctg gctgagttac gctctgacgg acaaattaat ctgctgaatg cgttgtacgt
     6361 tgcggcgaat tatccgatca acgaagtaac gctctttttc aataaccgat tgtatcgcgg
     6421 caaccgcact accaaagccc atgccgatgg ttttgatgcg tttgcctctc caaaccttcc
     6481 tccgttactg gaagctggta tccatattcg tcgtttgaat acgccacccg ccccgcacgg
     6541 tgaaggggaa ttaatcgttc atccaatcac gccacaacca attggcgtag tgacgattta
     6601 tccagggatt tctgctgacg tcgtgcgcaa ttttctgcgc caaccggtga aagcattgat
     6661 tctgcgctcc tatggcgtgg gtaatgcgcc acaaaacaaa gccttcctgc aggaattaca
     6721 agaagccagc gatcgcggta ttgtggtggt caacctgaca caatgtatgt ccggtaaagt
     6781 gaatatgggt ggttatgcca ccggtaacgc cctcgcccat gccggcgtta ttggcggtgc
     6841 agatatgact gtagaagcca cgctaaccaa actgcattac ctgctgagcc aggaactgga
     6901 tactgaaacc attcgcaagg ccatgagcca aaacctgcgc ggcgaactga cgccggatga
     6961 ttaaggagac tgcaatgccc catcgcgccc tgttactggt cgatttacaa aatgatttct
     7021 gtgctggtgg cgcgctcgcc gtgccggaag gtgacagtac ggtagatgtc gctaaccgcc
     7081 tgattgactg gtgccagtcg cgcggtgaag cggttatcgc cagtcaggac tggcacccgg
     7141 cgaatcacgg cagttttgcc agtcagcacg gtgtagagcc ttatacgcca ggccaactcg
     7201 acggtttgcc acaaaccttc tggccagatc actgtgtgca gaacagtgaa ggcgcacaat
     7261 tacatccgtt actgaaccaa aaagcgatcg cagcggtgtt ccataaaggc gaaaatcctt
     7321 tagttgacag ttacagtgcc ttttttgata acggccgtcg gcagaaaacc tctctcgatg
     7381 actggttacg cgatcatgaa atcgatgaat tgatcgttat gggcctggct actgactatt
     7441 gcgtgaagtt taccgtgctg gacgcgttac agttaggtta taaggtaaac gtgattaccg
     7501 atggttgtcg tggcgtgaat atccagcccc aggacagtgc gcacgcgttt atggagatgt
     7561 cagcagctgg ggcaacgcta tatacgctgg cagactggga agagacgcag gggtaatatt
     7621 agttgaattc atctctggcc tacatttcct caatgacatt gtaggccaga taatacttgt
     7681 cagtgttgca tttaacctac ttgcagctaa aacacgacgt cctaacaagt atatttgccc
     7741 tcccttaata acaggcttaa atactagata actcattcac ttaaacatag caataggctt
     7801 tgctgagata ccaaaatctt cttttaactg ctgtttgctc ttcatgacca tctgaccatt
     7861 tgtgtcaatt gtcatatgct gtgcttcggt attatggcgg gcttgccaca gcatcaccag
     7921 ttgcaggcaa ttctcttttt gttcctcggt caacgcaacg ccatcaggcc atttccccag
     7981 ttcaacggcg gtcgacaaac gctggtatac ctcaggcgtc atgctgttga taatgtcatc
     8041 aagattcata atttttccgc tcctgtggaa taagttgctg aatcgttttt tcaaccgttg
     8101 atttcttcgc cgttttcgcc atcagtgaag cgtaaagaag cagagttaac acaataacgt
     8161 tcgcctgttg gttgcggccc gtcgggaaag acatgcccca gatgggcatc acagttaccg
     8221 caacgaattt ctatgcgctg cattccatgt gacaagtctt tgatataacg aatggattct
     8281 tcactcaccg gttcgtagaa actgggccag ccacagccgg aatcatactt ggtttcggaa
     8341 tgaaacagcg gggcatcgca gatcaaacag tgatatacgc cgtcacgctt gttatgcagt
     8401 aaacgacccg taaatggcgg ttctgtccca tgattctgcg tcacgtaaaa ctgcatctcg
     8461 gacaaatttt ttttcagttc ttctgccgaa ggtttattag ccatttgctc acatctcact
     8521 ttaatcgtgc tcacattacg tgactgattc taacaaaaca ttaacaccaa ctggcaaaat
     8581 tttgtcctaa acttgatctc gacgaaatgg ctgcacctaa atcgtgatga aaatcacatt
     8641 tttatcgtaa ttgcccttta aaattcgggg cgccgacccc atgtggtctc aagcccaaag
     8701 gaagagtgag gcgagtcagt cgcgtaatgc ttaggcacag gattgatttg tcgcaatgat
     8761 tgacacgatt ccgcttgacg ctgcgtaagg tttttgtaat tttacaggca accttttatt
     8821 cactaacaaa tagctggtgg aatatatgac tatcaaagta ggtatcaacg gttttggccg
     8881 tatcggtcgc attgttttcc gtgctgctca gaaacgttct gacatcgaga tcgttgcaat
     8941 caacgacctg ttagacgctg attacatggc atacatgctg aaatatgact ccactcacgg
     9001 ccgtttcgac ggtaccgttg aagtgaaaga cggtcatctg atcgttaacg gtaaaaaaat
     9061 ccgtgttacc gctgaacgtg atccggctaa cctgaaatgg gacgaagttg gtgttgacgt
     9121 tgtcgctgaa gcaactggtc tgttcctgac tgacgaaact gctcgtaaac acatcaccgc
     9181 tggtgcgaag aaagtggtta tgactggtcc gtctaaagac aacactccga tgttcgttaa
     9241 aggcgctaac ttcgacaaat atgctggcca ggacatcgtt tccaacgctt cctgcaccac
     9301 caactgcctg gctccgctgg ctaaagttat caacgataac ttcggcatca tcgaaggtct
     9361 gatgaccacc gttcacgcta ctaccgctac tcagaaaacc gttgatggcc cgtctcacaa
     9421 agactggcgc ggcggccgcg gcgcttccca gaacatcatc ccgtcctcta ccggtgctgc
     9481 taaagctgta ggtaaagtac tgccagaact gaatggcaaa ctgactggta tggcgttccg
     9541 cgttccgacc ccgaacgtat ccgtagttga cctgaccgtt cgtctggaaa aagctgcaac
     9601 ttacgagcag atcaaagctg ccgttaaagc tgctgctgaa ggcgaaatga aaggcgttct
     9661 gggctacacc gaagatgacg tagtatctac cgatttcaac ggcgaagttt gcacttccgt
     9721 gttcgatgct aaagctggta tcgctctgaa cgacaacttc gtgaaactgg tatcttggta
     9781 cgacaacgaa accggttact ccaacaaagt tctggacctg atcgctcaca tctccaaata
     9841 agttgagatg acactgtgat ctaaaaagag cgacttcggt cgctcttttt tttacctgat
     9901 aaaatgaagt taaaggactg cgtcatgatt aagaaaattt ttgcccttcc ggtcatcgaa
     9961 caaatctccc ctgtcctctc ccgtcgtaaa ctggatgaac tggacctcat tgtggtcgat
    10021 catccccagg taaaagcctc ttttgcatta cagggcgcac accttctctc gtggaaacct
    10081 gcgggtgaag aagaagttct gtggttgagc aacaacacac cgttcaaaaa tggcgtcgct
    10141 attcgcggtg gcgtaccggt ttgctggccg tggtttggtc cggcggcaca acaaggtctg
    10201 cctgcgcacg gttttgcccg caacctgccg tggacgctga aatcacatcg tgaagatgct
    10261 gatggcgtag cgctgacttt tgaattgacg caaagcgaag agacgaaaaa attctggccg
    10321 cacgacttta cgctgttagc gcatttccgc gtgggtaaaa cctgtgaaat cgatcttgaa
    10381 tcacatggcg aatttgaaac cacctctgcc ctgcatacct actttaacgt gggtgatatc
    10441 gctaaggtaa gcgtcagtgg gctgggcgat cgcttcattg ataaagtaaa tgacgcgaaa
    10501 gaagatgtac tgaccgacgg tattcagacc ttccctgacc gtaccgatcg cgtgtatctg
    10561 aatccgcaag attgcagcgt gattaatgat gaagcgctga atcgtattat cgccgtaggc
    10621 caccagcatc atctgaacgt tgtcggctgg aacccgggac cggcgctttc agttagcatg
    10681 ggcgacatgc cggatgatgg ctacaaaaca tttgtttgtg tagaaacggc ttacgcttca
    10741 gaaacgcaaa aagtgaccaa agagaaacct gcacatctgg cgcaatccat tcgcgttgcg
    10801 aaacgttaat ttgcgttaat gttgtgtgcc gggtgcgatg catccggcac gtatcaacat
    10861 cacaccatat ccagcgcagt ttttcctttt ggtgccggat acgctttatc cagcatggtt
    10921 aattccgctg acgaaagttc gacctcaagc acagccgcat tctgttggac atgggcaatc
    10981 gtggccgctt ttggaatcgc catcacaccc tgatggctga ttacccacgc caacaatact
    11041 tgtgccgcgc tgatattgtg agcatgtgca atttcgttga ctaccgcgtt ttttaacagt
    11101 ccattgcgca accgcccggc ctgggctaat ggactgtaag ccatcaccgg catctgctgt
    11161 tgctggcacc aggggagtag atcgtactca atccctcgtg aaccgagatg gtaaagcacc
    11221 tgattagtgg cacactgatt tccccccggc agctgccaga gttcctgcat atcagcatga
    11281 tcaaggttag aaacgcccca gcggcggatt tttccctggg cgatcaattt ttccatcgct
    11341 gccacagtct cttcaaaagc gaaactgcca gaccagtgta ataagtagag atcgagataa
    11401 tcagtattga gacggcgtaa actggcttcg catgcattta tcgctttttg cccgccagca
    11461 ttccacggat agactttaga gacgagaaag acgttatcgc gcagaccggt taatgcttcc
    11521 ccaaccacct tttcggcacc gccatcggca tacatttcgg cggtatcaat gagggttaaa
    11581 ccgagctcaa tgcccgcgcg tagtgcagca acttctgttt tgcgctgact ggcatcttcg
    11641 cccatatacc atgttccctg ccctatggct ggcagtgaga catcgccact aaattgaatc
    11701 attttttgtt gcattgtttc ctcccaggta ttgcaccacc gtaatgcaaa acagggcgtg
    11761 acgccctgtt tttatgcaca aaatgccctg gaaagatgca ttatcagaat ttgtaggtga
    11821 tcccggtaga aatcaggcca gtccaggatt tatccaccat cgggctgtca gtaacttcat
    11881 cagacagacg ggtatagcgc gcggtaccgt aaacactcca gtcgccgagg aagttgtagc
    11941 tggcgctcag ctccaggtaa gggctccagc tgtcgttcgg gttatagcca cgcagaccgc
    12001 tgcgagcgga ctctttgcgc gatacgccat aatagtattc gttctggttt tcgctgttcc
    12061 actgcacacc gatacccggg gtcacggtca ggccgccgtt ggtgtaacga tacaaccagg
    12121 ccatatccca gacgatgccg ttgctgttat ccagggtatc gccagccagg gtggtacgca
    12181 ggtaaccgta ctgggtaaag tgagcataag acagaccagc catcatggtg ctcttacggt
    12241 catccaggtg acgcatttgg tgatcgccac tgtctttcgc tttgaagtaa agcggcgacc
    12301 aataagcggt aattgaaagt ttgtccgttg cgtcattcca caggtagtaa ccaccaccta
    12361 agccacggaa ccagaagtta tcgccttcat agttgattac cggtactggg taaacatcgg
    12421 tatcgtaatc tttatatggg tgctcaacga cacctacgcc tgcgcccagg gaaaatttac
    12481 cttcagcgtg cgctacgcct gcagacgttg cgataagcac tccaagtgcc agaagtttga
    12541 gtttggtcac aattaatcat tccttaaaca aatgtttagc gggcgacaaa gtttacccgt
    12601 caatacatcg aaacccaacc tttttacgtt ttcatttttt aaagtaactg tttaattttc
    12661 ctgacgcgga tgacaccgcg cttacagcca aatgaattta gcgttactgg ctaccctggt
    12721 ctttacatta attatgcaaa atttatggat gagttgttga tatgccattg aaattaagaa
    12781 agccgtgcag gcaagttttc catttgccat ctacgcttaa ttttgaaggt gtatcaccgg
    12841 gcacgttgtt ctcatcgtcg ataaaatggc atgagagttg ctgtgtttta gcaagagacg
    12901 tcgttcagtt tacctcttcc gggagcctct actattcata tgaacggctc ttaacctgtg
    12961 ctaaaaaacg aaaggacggc ataccatgaa tatattcgat cactatcgcc agcgatatga
    13021 agctgccaag gacgaagagt tcacactgca ggagtttctt accacttgtc ggcaagatcg
    13081 cagtgcttat gccaacgcgg ctgagcggct attgatggct atcggtgagc ctgtcatggt
    13141 cgatacagcc caggaaccca gactttctcg actcttttct aaccgggtca ttgcacgtta
    13201 tccggcattt gaagagtttt acggcatgga agacgcgatt gaacagattg tctcttatct
    13261 gaaacacgcg gctcaggggc tggaagagaa gaaacaaatc ctgtatctgc tggggcctgt
    13321 gggtgggggt aaatcatcgc ttgctgagcg actgaaatca ttaatgcagc tcgtaccgat
    13381 ttatgtattg agcgccaacg gtgagcgtag cccggtcaac gatcatccgt tctgtctttt
    13441 caatccgcag gaagatgctc agattctgga aaaagagtat ggcattcctc gccgttatct
    13501 cggcaccatc atgtcgccat gggcggcaaa acgcctgcat gaatttggtg gcgatatcac
    13561 taagttccgg gtagtgaagg tctggccgtc aattctgcaa caaattgcta tcgccaaaac
    13621 ggaacccggt gatgagaaca accaggacat ctccgcgctg gttgggaaag tcgatattcg
    13681 taaactcgaa cactacgcgc agaatgaccc ggacgcctac ggctattccg gtgcgctgtg
    13741 ccgcgccaac caagggatca tggaatttgt cgagatgttt aaagcaccga taaaagtgct
    13801 gcatccgctg ttaaccgcca ctcaggaagg taactacaac gggacggaag gtatctccgc
    13861 cctgccgttc aacgggatta ttctcgccca ctcgaacgaa tccgaatggg tcactttccg
    13921 taataacaaa aacaacgaag ccttcctcga ccgtgtttac atcgtgaagg tgccgtattg
    13981 cttacgcatt tccgaagaga tcaaaatcta cgagaaattg cttaatcaca gtgaattgac
    14041 tcacgccccc tgcgcccctg gcacgctgga aacgctgtca cgtttttcca ttctttcgcg
    14101 ccttaaagag ccagaaaact ccagcattta ttcaaagatg cgggtttatg atggcgaaag
    14161 tctgaaagac accgatccca aagccaagtc gtatcaggaa tatcgtgact acgccggtgt
    14221 cgatgaaggg atgaacggtc tgtcgacgcg ttttgcgttt aagatcctct cccgcgtgtt
    14281 caacttcgat catgtagaag tggcggcaaa cccggtccat ctgttctacg tcctggaaca
    14341 gcagatcgag cgcgagcagt tcccacaaga gcaggcagaa cgctatctgg agttcctgaa
    14401 aggttatctg atcccgaaat atgccgagtt tatcggcaaa gagatccaga cggcctacct
    14461 tgaatcctat tccgaatatg ggcaaaacat tttcgaccgt tatgttacct acgcggattt
    14521 ctggattcag gatcaggagt atcgcgatcc ggataccggg cagctgtttg accgcgagtc
    14581 tcttaacgcc gagctggaga aaatcgagaa accggcgggg atcagtaatc caaaagattt
    14641 ccgcaacgag attgttaact tcgtactgcg cgccagagcg aataacagcg gacgcaatcc
    14701 gaactggacc agctatgaaa aactgcgcac ggttatcgag aagaaaatgt tctccaatac
    14761 cgaggagctg ttgccggtta tctcgtttaa cgccaaaacg tcaaccgacg agcagaagaa
    14821 acacgacgac tttgtcgacc gtatgatgga aaaaggctac acccgtaaac aggtgcgttt
    14881 actgtgcgaa tggtatttgc gcgtacgtaa atcgtcttaa caaccctggc ccggtacaca
    14941 tgttaccggg cctacaacga cagcgaaccg tgggcctgag aagcggcaac acaggcgtag
    15001 catacagttg gcaaatgtag tacggggggc atatgacctg gtttattgac cggcgtctga
    15061 acggcaaaaa caaaagcatg gtgaatcgcc agcgtttttt acgccgttat aaagcgcaaa
    15121 ttaaacagtc gatctccgag gccattaata agcgttcggt gactgacgtc gacagcggcg
    15181 aatccgtatc cattcccacg gaagatatta gcgaaccgat gtttcatcag gggcgtggcg
    15241 gtctgcgcca ccgcgtgcat ccgggcaatg accatttcgt ccagaacgac cgaattgaac
    15301 gtccccaggg tggcggcgga ggttccggta gtggtcaggg ccaggccagc caggatggtg
    15361 aaggtcagga tgaatttgtc tttcagattt cgaaagatga gtatcttgat ttgctctttg
    15421 aagatttggc cttaccgaat ctgaaacaaa accaacaacg ccagctgacc gaatataaaa
    15481 cgcatcgggc gggttatacc gctaacggcg ttccggcaaa tatcagcgtt gtgcgctcct
    15541 tgcagaactc actggcgcga cgcacagcca tgacggcagg caagcggcgg gaacttcatg
    15601 cactggaaga gaatttggcc atcatcagca acagtgaacc tgcgcagctg ctggaagagg
    15661 aacgtctgcg caaagaaatt gcagaattac gtgccaaaat tgaacgcgtc ccttttattg
    15721 acaccttcga tttacgttac aagaactacg agaagcggcc cgatccctcc agccaggcag
    15781 tgatgttttg cctgatggac gtttccggtt caatggatca atccactaaa gatatggcta
    15841 agcgttttta tattctgctg tatctgttcc tcagcagaac gtataagaac gtggaagtcg
    15901 tatacatccg ccatcatacc caggcgaaag aagtcgatga acatgagttt ttctactcgc
    15961 aggaaacagg cggcaccatt gtttccagcg ccctgaaact gatggatgag gtagtgaaag
    16021 agcgttataa cccggcacag tggaatattt acgctgcaca agcatcggac ggcgataact
    16081 gggccgatga ctctccgctt tgccatgaaa tcctggcgaa aaaattatta cctgttgttc
    16141 gttattacag ctatatcgaa attacccgtc gtgcacatca gacattgtgg cgagaatatg
    16201 agcatctgca atctactttc gacaactttg cgatgcagca catccgcgac caggatgata
    16261 tttatccggt gttccgtgaa ctgtttcata aacaaaatgc aacagctaaa gactaaaact
    16321 atcagccagg tcattatcgc ctggctgatt tttagcttac tgtaaattat ctcatttatt
    16381 acatacatta gcttacaatc gctttaaata tgacagcgta acctttacat aatttagttc
    16441 cagaaaacaa tcattcggaa aaatgattca gtcaacacgt atttccatgg ggttattctt
    16501 taaatattgt ttatcgttaa cgaaaattga tcctggtcaa aactatatat ctctgccatc
    16561 aataaaatcc agcactcaca ttgctctcct ttttatggtt tctatgggta cacaaaaatt
    16621 aaaagctcaa agctttttta ttttcagttt attgctgacg ttaattttat tttgcatcac
    16681 cacattatat aacgaaaaca caaatgtaaa actcatccca cagatgaatt acctgatggt
    16741 tgttgtggct ttgtttttcc ttaacgccgt catttttctt ttcatgttaa tgaaatattt
    16801 cactaacaaa caaattttac caacactcat tttaagc
//
LOCUS       DAZFWL020000099        16695 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFWL020000099 DAZFWL020000000
VERSION     DAZFWL020000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.099"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(73..225)
                     /locus_tag="ACHKNP_RS17265"
                     /old_locus_tag="pgaptmp_003453"
     CDS             complement(73..225)
                     /locus_tag="ACHKNP_RS17265"
                     /old_locus_tag="pgaptmp_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4705563.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(490..1224)
                     /gene="cysH"
                     /locus_tag="ACHKNP_RS17270"
                     /old_locus_tag="pgaptmp_003454"
     CDS             complement(490..1224)
                     /gene="cysH"
                     /locus_tag="ACHKNP_RS17270"
                     /old_locus_tag="pgaptmp_003454"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU4705564.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1298..3010)
                     /gene="cysI"
                     /locus_tag="ACHKNP_RS17275"
                     /old_locus_tag="pgaptmp_003455"
     CDS             complement(1298..3010)
                     /gene="cysI"
                     /locus_tag="ACHKNP_RS17275"
                     /old_locus_tag="pgaptmp_003455"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HGU4705565.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDHEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3010..4809)
                     /gene="cysJ"
                     /locus_tag="ACHKNP_RS17280"
                     /old_locus_tag="pgaptmp_003456"
     CDS             complement(3010..4809)
                     /gene="cysJ"
                     /locus_tag="ACHKNP_RS17280"
                     /old_locus_tag="pgaptmp_003456"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HGU4705566.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5128..5490
                     /gene="queD"
                     /locus_tag="ACHKNP_RS17285"
                     /old_locus_tag="pgaptmp_003457"
     CDS             5128..5490
                     /gene="queD"
                     /locus_tag="ACHKNP_RS17285"
                     /old_locus_tag="pgaptmp_003457"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HGU4705567.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5568..6839
                     /gene="ygcN"
                     /locus_tag="ACHKNP_RS17290"
                     /old_locus_tag="pgaptmp_003458"
     CDS             5568..6839
                     /gene="ygcN"
                     /locus_tag="ACHKNP_RS17290"
                     /old_locus_tag="pgaptmp_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4705568.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6830..7090
                     /gene="ygcO"
                     /locus_tag="ACHKNP_RS17295"
                     /old_locus_tag="pgaptmp_003459"
     CDS             6830..7090
                     /gene="ygcO"
                     /locus_tag="ACHKNP_RS17295"
                     /old_locus_tag="pgaptmp_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU4705569.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCCLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7107..7682
                     /gene="ygcP"
                     /locus_tag="ACHKNP_RS17300"
                     /old_locus_tag="pgaptmp_003460"
     CDS             7107..7682
                     /gene="ygcP"
                     /locus_tag="ACHKNP_RS17300"
                     /old_locus_tag="pgaptmp_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HGU4705570.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7830..8690)
                     /gene="ygcQ"
                     /locus_tag="ACHKNP_RS17305"
                     /old_locus_tag="pgaptmp_003461"
     CDS             complement(7830..8690)
                     /gene="ygcQ"
                     /locus_tag="ACHKNP_RS17305"
                     /old_locus_tag="pgaptmp_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU4705571.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAVKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8687..9466)
                     /gene="ygcR"
                     /locus_tag="ACHKNP_RS17310"
                     /old_locus_tag="pgaptmp_003462"
     CDS             complement(8687..9466)
                     /gene="ygcR"
                     /locus_tag="ACHKNP_RS17310"
                     /old_locus_tag="pgaptmp_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGU4705572.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRIEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9444..10853)
                     /gene="ygcS"
                     /locus_tag="ACHKNP_RS17315"
                     /old_locus_tag="pgaptmp_003463"
     CDS             complement(9444..10853)
                     /gene="ygcS"
                     /locus_tag="ACHKNP_RS17315"
                     /old_locus_tag="pgaptmp_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4705573.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10875..12329)
                     /gene="ygcU"
                     /locus_tag="ACHKNP_RS17320"
                     /old_locus_tag="pgaptmp_003464"
     CDS             complement(10875..12329)
                     /gene="ygcU"
                     /locus_tag="ACHKNP_RS17320"
                     /old_locus_tag="pgaptmp_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HGU4705574.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPQEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGTDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12399..13184)
                     /gene="ygcW"
                     /locus_tag="ACHKNP_RS17325"
                     /old_locus_tag="pgaptmp_003465"
                     /pseudo
     CDS             complement(12399..13184)
                     /gene="ygcW"
                     /locus_tag="ACHKNP_RS17325"
                     /old_locus_tag="pgaptmp_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13502..14779
                     /gene="yqcE"
                     /locus_tag="ACHKNP_RS17330"
                     /old_locus_tag="pgaptmp_003466"
     CDS             13502..14779
                     /gene="yqcE"
                     /locus_tag="ACHKNP_RS17330"
                     /old_locus_tag="pgaptmp_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HGU4705575.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEVRTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14806..16284
                     /gene="ygcE"
                     /locus_tag="ACHKNP_RS17335"
                     /old_locus_tag="pgaptmp_003467"
     CDS             14806..16284
                     /gene="ygcE"
                     /locus_tag="ACHKNP_RS17335"
                     /old_locus_tag="pgaptmp_003467"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HGU4705576.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGSVVCEGKGLLQPMHTPYAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         3994 a         4374 c         4191 g         4136 t
ORIGIN      
        1 aaattttaaa aaagggtgca ggagaccaaa ccatcacaat tcagtacgcg gggttttacc
       61 cccgcgtggt ggctacttct tcgattcgta agcgagaact gccttaaact ccataccacg
      121 ttctctgata ctcaactcac acaacgagtc acctaccatc agcgtgaatc ccagtactgt
      181 acaacacagt acgatgattg ccacaagggc atattttgtc agcatgtctt gcctccctgc
      241 aaagaagaga ctaacattcg aattgtttgg gttcgaacgt aagcctcagg ttgatagaaa
      301 tatcgcctgg ggcttttgtc catctggaac ctcgcgaatg cttaacgcca gacagcctca
      361 agcacccgac gccattctat acctgataat tcttccagcg gcgttcacgt ttaaaatcag
      421 gaatactgct cgcaaaataa aaacgccccc atcatttctg acagaggcgt ttaatttatc
      481 cggcaatatt tacccttcgt gcaatccaca ttcccttttt aagccaaaga aacgtgtttc
      541 ttcttccgac atgccaggtt cccatttacg ggttgtatgg gtatcgccca ccgataaata
      601 tccttcgtcc cataatgggt gatatttcag gccatgtttt tgcagatact gatatatcgt
      661 tcggttatcc cagtcgataa tcggcagcac tttaaatacg ccacgctgaa ttgccagcac
      721 cggtaaattg gcgcggctgc cggattgttc gcggcgcagg ccagcaaacc acgtttgcgc
      781 attcagttct ttcagcgccc ggttcatcgg ttcgactttg ttgatgtcat tgtacttttc
      841 aatgccttca acgccctgtt cccacagttt gccgtagcgt gcttcctgcc aggcggcgct
      901 ttcggtagcg cggtacactt tcaggttgag cttgagtttg tccgttaact cgtcaataaa
      961 gcggtaggtt tccgggaaca gatagccggt gtcggtgagg atcaccggaa tatccgggtg
     1021 aatctgattc accagatgca ggctcaccgc cgcctgaatg ccaaagctgg aagaaagtac
     1081 atattcaccg ggcagattat ccagcgccca ggcaacgcgg ccttcagcgt ccagtttttc
     1141 cagttcggcg ttagtttctg ccagcgccag aatgcgatct accttcggca gttcgttcag
     1201 ggcgtttaga tcgagtttgg acatagattc ctcactgttt gccttgcctg atgcgacgct
     1261 cacgcgtctt atcaggccta caagaccggg ctgatggtta atcccacaaa tcgcgcgccg
     1321 gatcgagcac cgggcgaatg atgcccgcac gcaccgtaaa gtcgccgaag ccttcacccg
     1381 cttcgcgctc tttcgcccag cgccctatca gttcatcaag cgacgccagg atttccggct
     1441 cggtgatgtt ttctttatac atccgtggga tacgtgtccc aatgcggttg ccgccaagat
     1501 gcaggttgta gcgacccggc gctttaccca ccaggcccac ttccgccagc atcgcgcgac
     1561 cacaaccgtt cgggcagcct gttacacgca tcacgatatg ctcatcgctg acaccatgtt
     1621 tcgccattaa attatcgatg ttgtcgataa aagacggcag gaaacgctct gcttccgcca
     1681 tcgccagcgg gcaagtcggg aacgagacgc aggccatcga gttttcacgc tgcggcgtga
     1741 cggcattcat taacccgctc tctttggcga tcttctcgat cttcgctttc tcgctttccg
     1801 gtacaccggc gatgatcaga ttctggttcg ccgtaatgcg gaaatcgcct ttgtggatct
     1861 tcgcgatctc cagcaggccg gttttcagcg gacgccccgg ataatcaagg atgcgaccat
     1921 tttcgataaa cagcgtcagg tgccagttat catcaatgcc cttaacccag ccaatacgat
     1981 cgcctcgtcc ggtgaactca tatggacgga tcggttcaaa tttgatcccc gcgcgacgct
     2041 ccacttccgc tttaaacgtc tcaaccccca cgcgctccag cgtgtatttg gttttggcat
     2101 ttttacgatc ggttcggtta ccccagtcac gctgagttgt cacgacggct tcggccaccg
     2161 ccagcgtatg ctccagcggc agatagccaa actcgctcgc cgtgcgggcg taggttttct
     2221 tgttgccgtg ttcgatagaa agcccaccgc ccaccagcag gttgaagcct accagcttgc
     2281 cgttttcggc gattgccacg aagttcatgt cgttggcatg cagatcgata tcgttctgcg
     2341 gcgggatcac taccgtggtt ttgaacttac gcggcaggta ggtctgacca aggatcggtt
     2401 cttcatcagt agtggcgact ttttcgtggt cgagccagat ctccgcatac gcgcgggtac
     2461 gaggcaacag atgctcagaa atcttcttcg cccactcgta cgcttccgcg tgcagctgcg
     2521 actcgtaagg gttcgaggtg cagagtacgt tacggttcat gtcgttagct gtcgccagcg
     2581 catcaagacc gaccgagtgc agcatctggt gcaccggttt gacgttcttt ttcagaatgc
     2641 cgtggaactg aaacgtctgg cggttggtca ggcgaatgct gccatagatg gtgttttcac
     2701 cggcaaattt gtcgatcgcc tgccactgtt tagtggtaat caccccaccc ggcagacgac
     2761 agcgaagcag catcgcgtgg cgcggctcca gcttctgttc agcacgttcg gcgcggatgt
     2821 cgcggtcatc ctgctgatac atgccgtgga agcggatcag caggaagttg tcgcccttaa
     2881 agccgccggt cagaccgtcg tttaaatctt ccgcaatggt gccgcgcagg tagttgcttt
     2941 caagcttcat gcgctcggcg tctgtcagtt ttccttcgac cactaaaggc cctggatgtt
     3001 tttcgctcat tagtagacat ctcgctgata acggcgctct acgcgcagct cacttaaaaa
     3061 ttcatccgcc gcttcggtgt ccatgccacc aaattcggca atcacttcca gaagtgcctg
     3121 ctcaacgtct ttcgccatgc gattagcgtc gccgcagaca taaatgtggg caccatcatt
     3181 gatccagcgc cacagctccg cgccctgttc gcgcagtttg tcttgtacgt aaactttttc
     3241 tttttggtcg cgcgaccagg caagatcgat acgtgtcagc acgccatctt tgacgtagcg
     3301 ctgccactcc acctgataca ggaagtcttc cgtaaagtgc ggattaccaa agaacagcca
     3361 gtttttacct ggcgcttcgt cggcggcgcg ttgctgcata aaggcgcgga acggcgcaat
     3421 accggtgcct gggccaatca taatcaccgg ggtttccggg ttagcgggca ggcgaaagtt
     3481 atcgttatgt tcgataaata cgcggacttc gccctcttct tccacgcgat ccgcgaggaa
     3541 gctggaggca ccaccggcac gggctcggcc ttccacgtcg taacgcacca caccaacggt
     3601 gacgtgtact tcgttctcga cttccgcctg cgaggaggcg atggaataca ggcgcggggt
     3661 cagcgggcgc agcagattaa tcagcgcttc ggcatccagt tgcgccggag agaaacgcac
     3721 catgtcgaca atcggcgtcg tcgcggcgta atgctgtaac ttcgctttat cgcccaccag
     3781 cggcagcagt gtttcgctgc gggtaagcgt ggcgtaattc tcaacaatgt tggcggtgtt
     3841 gacggtcagt tcgaagtgcc actgtagcgc ttcgttcaga ggcaacgttt tgccctcgac
     3901 ggtgacaggt tcatcgcctt tcagccacag cagttcgaca agttctttca ccagtgccgg
     3961 atcgttctga taccagacgc ccagcgcgtc acccggctgg taacgcaggc ccgagtcacc
     4021 taagtcaatt tcgatatggc gaacgtcttt ttcagagtta cgcccggtaa ttttctggtt
     4081 aacagagagg ctagccacca gcggcgcgtc tttgctgtac gggctggtgt ggatttcatt
     4141 taccgcgcca gtagcgacgg attgcgaagg tgccgcgaca ggcgcacgcg atttaagcgc
     4201 atcaaccacg cgggcgcgcc actcgctggc agcagcctgg tattcaacat cggcatcgac
     4261 acggtcgagc aggcgttcac cacccagttc cgccagcttg ctgtcgaaat ctttcccgga
     4321 ctggcagaaa aattcataag agctatcgcc gaggctaaac acggcaaacg cggtgttttc
     4381 cagctttggc gcttttttgg agaacaggaa cttatgcagc gcgacggctt cttccggcgg
     4441 ttccccttcc ccttgcgttg acgtcactac gatgagcagt ttttcgctgg cgatttgttt
     4501 gaatttatag tcgcccgcgt tcaccagctt aacgttcagt tttgctgcta ataaatcatc
     4561 gcgtaatgct tcagcaaccc ggcgcgcatt gccggtttgc gaggcggaga taatagttat
     4621 acccggcatt tctgcggctg gcgctggcgt cgctgcaagc gcagcaggct gctgattgag
     4681 cacgccccag aaatagccag aaacccaggc aagctgggtg ggagttaaat cggtcgtggc
     4741 cgcctgaagg cgtgccagtt gctccgggtt caacggaagc aacgcggaag gtgggacctg
     4801 tgtcgtcatg cgtcgttatg ttccagtaag caaagctgtt tctgcgccct gtcagcgccc
     4861 ataaaacaga agagaaggta aggttaacgg ggtgaacggt gtggattaaa gacgggatag
     4921 cgataactaa taaccaaatc gactaacctg ttttagcaat agtctttaac aacaaaatag
     4981 attaaccaac ctaatgaaaa acaaatgaat ttagccaatc attaagataa atcagcgatt
     5041 ttgcgcaaca aagtcgtttt agataatgcg aaaaaacagc ctttccggta ctctacggcg
     5101 gttttatcgt cctgtagaga aattatgatg tccaccacgt tatttaaaga tttcaccttc
     5161 gaagccgctc accgcttacc acacgtcccg gaagggcata aatgtggtcg cctgcacggg
     5221 cattccttta tggtgcgact ggaaattacc ggggaagtcg atccgcatac gggctggatt
     5281 atcgatttcg ctgaactaaa agcggcgttt aaaccaacct acgagcgcct cgatcaccat
     5341 tatctcaatg atattccagg tctggaaaac ccaaccagcg aggttttagc aaaatggatt
     5401 tgggatcagg ttaaacccgt tgtgccgctg ttaagtgcgg tgatggtaaa agaaacctgt
     5461 accgcaggtt gtatctatcg cggcgaatga taagagtgtg tcggcggtca atttccctta
     5521 agtaacgcta tgttagggtg ttgtgttctg gatatctggg gcatgacatg gaagacgact
     5581 gcgacattat tattattggt gccggtattg caggcaccgc ttgcgcgtta cgctgcgcgc
     5641 gagcgggttt atccgttttg ttactggaac gcgctgaaat ccccggcagc aaaaatcttt
     5701 ccggcgggcg gttatatacc catgcactcg cggaactcct cccgcaattt catctgaccg
     5761 cgcctcttga acgacgcatc actcacgaaa gcctttccct gttaacgccg gatggcgcaa
     5821 cgacgttttc cagcttacag cccggcggtg aatcctggag tgtattacgt gcacggttcg
     5881 atccgtggct ggttgccgaa gccgaagaag aaggtgtcga atgcatcccc ggagcgacgg
     5941 tggatgcact gtatgaagaa aacggcaggg tgtgtggtgt catttgtggt gacgatattc
     6001 tccgtgcccg ttatgtggtg ctggcagaag gtgccaacag cgtcctggct gagcgtcacg
     6061 ggttagtgac tcgtcctgct ggtgaagcga tggcgttggg gatcaaagaa gtgctgtcgc
     6121 tggaaccatc cgctattgaa gaacgttttc atctggagaa taacgaaggc gcagcgttgc
     6181 tgttcagcgg caggatctgt gatgacttac ccggcggcgc atttctttat actaatcaac
     6241 aaacgctctc gttagggatt gtttgcccgc tctcttccct tacgcaaagt cgtgttccgg
     6301 caagcgagct gctgactcgc tttaaagcgc atccggcagt gcgcccgctt atcaaaaaca
     6361 cggaatcact ggagtatggt gcgcatctgg tgccagaagg tggcttgcac agtatgccgg
     6421 tgcaatacgc cggtaacggc tggctgctgg tgggcgatgc gttgcgcagt tgcgtcaata
     6481 ccggaatttc cgtgcgcggc atggatatgg cgctgactgg cgcgcaggcg gcggcacaaa
     6541 cgctgataag cgcctgccag caccgcgagc cgcaaaatct gtttgcgctt tatcatcaca
     6601 acgtagagcg cagcctgcta tgggatgttc tacaacgtta tcagcatgtt ccggcgcttt
     6661 tgcaacgccc tggctggtat cgggcgtggc ctgcgttaat gcaggatatt tcccgcgatt
     6721 tatgggatca gggtgataaa cctgttccac cgctgcgcca gttattctgg cgtcatttac
     6781 gtcgtcatgg cctgtggcat ctggcgggcg atgttatcag gagtctgcga tgtctgtagc
     6841 ccataatctc tggcgcgttg ctgatgcgcc gcacattgtt ccggctgact ccgttgagcg
     6901 tcagatggta caacggttga ttaacgcctg tccggcaggt cttttttcgc tcacaccgga
     6961 aggtgactta cgcgttgatt atcacggctg cctggagtgt ggcacctgct gtttgctgtg
     7021 cgacgaatcc acactacaac agtggcgcta tccgccttcc ggattcggca tcacctaccg
     7081 ctttggataa ataataagga taatttatgc ccctcttaca cctgctccgc cagaatccgg
     7141 tgattgctgc cgttaaagat aatgccagcc tgcaactggc aatcgactct gaatgccagt
     7201 ttatttccgt gttgtacggc aatatctgca ccatcagtaa tatcgttaaa aagattaaga
     7261 acgccgggaa atatgctttt attcatgttg atttgctgga aggcgcgtca aataaagaag
     7321 tcgtgattca gtttttgaaa ctggtgaccg aggcggacgg cattatcagt accaaagcct
     7381 caatgttgaa agcggcaaga gcggaaggtt ttttctgtat tcatcgcctg tttattgttg
     7441 attcaatttc gtttcacaac attgataagc aagttgcgca atcgaatccg gattgtattg
     7501 agatcctgcc aggctgtatg cccaaagtgc tgggctgggt gacagagaaa atccgccaac
     7561 cgctgattgc cggtgggctg gtgtgcgatg aagaagatgc gcgtaatgcg attaacgcgg
     7621 gtgtcgtggc gctttccacc acgaataccg gggtctggac gttagcgaaa aaattacttt
     7681 gacgggataa tcgtaaccaa ttgaatttgg tttgatttgt aggccgcacg ccacatccga
     7741 cattcagcgc ctgatgcgac gcttgacgcg tcttatcagg cctacaagtc cccaggccag
     7801 aaccgtaggc aggatcatcc ggcaacacgt tattggcaat ctgcgtgaat gtttgtcacc
     7861 agtgcctcca gcaccacttt ccagtcatca accacgccaa catctgcctg tgaaaatacc
     7921 gcagcgctgg cgtcatgatt aatcgccacc acaaacttgc tattgcgtac gcccgccatt
     7981 aacgctgctg cgccagaagc gccaacaaca atacacacct caggtgccag cagatgtccg
     8041 gaaataccaa tcactttttc agcatcaacg ccaccgttca tcactcgcgc ccgactgtag
     8101 cccacttcgg ctcccagctt ttctgccagc atagcgattt cctggttatc tgcctcaccg
     8161 ccctgcccca ccaccagcac ccgtctggcc tcagctaatg ggtcacgggt aacatttttc
     8221 agatcctctg tgctaaccag ccagtcggga agcgcgccgg ggacaatgtt cagttgctgc
     8281 ataccagagg gcaacgtggc gtttttcact gctcccgcct gacgcgccag ggaaaggcat
     8341 agcgggcgtt tttctgtttg cagcgtcgcc gttagcgcat ttccccagtg ggatttgcgc
     8401 acgcttacgg ttggaatatc taacgaagtc acctggcaga tgcttgcacc atgtaaacgc
     8461 caggccagcc gggtcgacag ttcatcgcca aacgttcccg gcggaaagag aacaacgtcc
     8521 gcaggcgttc gctgccactg ttccaccaac gcatccagca cctgttccgc cactacaggc
     8581 tgaggttcaa tctgccagtg cgccagggta caaccgctga aatcctgcgc cgccagccag
     8641 cttgctattg cggcattctc ctgatttatt gtcacaattg cgatattcat ggctgcatcc
     8701 tttggcgtaa atagtcctgc catagctttt gagctttttc tgctaccgtt tgcccatcaa
     8761 tcagcgtcgc tccgcgtcgt tgctctgctc ttgccagttg cagacactgc atagctggca
     8821 tttcggcagc aacagtttta cggataattt ctgctttccc cgcagccatt cgctggcgca
     8881 ttcccggtac tggcaaggcc acttcaccgc actgacgcac ggcaatcacc gcaggcaagc
     8941 gaacccggca acaacgcaaa ccatgttcaa tacgctgttc gagggtgata aacagcgcgt
     9001 cgagagtgaa acgctccacc tgggtgaagc agggccagcc cagcatttcc gccagcaaaa
     9061 atggcgtttg cccattctgc ccttcgctgc tttggcagcc ggtaattatc agatccagcg
     9121 gattctgatg ctgccattcg gcaatatggc gagcgacaaa ttccggcgca aagcgcagat
     9181 ctgccgccgt ctccagcaat acagcttcct caaacccaag agccatgaga tagcgcagcc
     9241 agtgcaatgc ccgttcatcc cccatgctca acgcggttaa agacatcggc gtaccgtttt
     9301 ttctctgcgc caacagcagc gcggcagcgg cctgttcatc agcaccgagt aaacttcgca
     9361 gtagcgaaat atccggtccg cttttaccct gagccgccgc ctgccactct ttttccgcca
     9421 gcattccggc atccggttcg gctttaaacg ctaacagaat gttcattcgc acctcctaca
     9481 tttcccgccg ccaccagcgg gagggcttta gtttctggtg cccatagcca ggtcacaaca
     9541 aaaccaacca acaacactgt cgccaggagc aataaggtga cttgcattcc ccactgcgcc
     9601 agcacccacg gcagcaggcc agtacttacc gccgcgccaa gtcgactcat ggcggtggca
     9661 aaaccgacgc ccagcgagcg aatgtcagta ggaaaacttt ccgcaggcaa aatgcccacc
     9721 agattactga ctgccgaaat ggtggtgctg aagagaacaa aaagcagcag cgttaatgaa
     9781 ctgccggaag gcaaacaggc cattactacc agcgttgccg ccagcagcaa aaaacttccc
     9841 agcaaaaatt tgcgatgtgc cagcaggtgc gtcagaacta atcccagcag cgcgcccaca
     9901 attaacaacg cattaagcat caggctggca gtcagcgcat cttccagacc aatagtctgg
     9961 gcgatagttg gcagccaggt ataaatcaca aaccatggga ttacgaggca gacaaagaag
    10021 acgctgttaa acgccgtgcg ccgccagtaa cgcgaagaga acaaggtttt gatgtgttta
    10081 tgggtcgccg ttaccacttc atcgcccagt aaaacatggg gaccaaaata gcgatgcacg
    10141 atagcgtgag cttctgcaaa acgcccctgg cgcagtagcc agcgtggtga ttctggcgtt
    10201 ccccagcgta ataacgtaat caacaacgcg ggcagagctg ccgatgccag tagccagcgc
    10261 caggcctccg ggttttcgga aataaagtga tgtccggcaa tacttgccag cacatagcct
    10321 acggtccaca ccacgctgaa tgcgcccagc aaaataccgc gatggcggcg cggggaaaat
    10381 tcagccagca aggtgtgacc tactgaataa tcgcctccca gaccaatgcc aatcaaaatg
    10441 cgcagtccaa taagatgctc tggcgtggtg gcaaaaaatt gtaaaaacga agcaagcgta
    10501 atcagcaaaa agctgaaggt gaagattttt tgccgaccaa tatggtcgga gatccaccca
    10561 agaaccaggc tgccaaggaa caaaccaagg agcgccgagc cgccgatcat tcccgccata
    10621 aacggcgtca gttgcatggc gggcgtaagc tgaataatgg cgtaaccaat gacgccgaga
    10681 acataaccgt cggtcaggtg tgcgccgaaa gtgagcgcag caatgcggca gtgaaaacgg
    10741 ttaagcggta aatcatccat tcgcaccggt gaagtgttca tctttacccc ttcattgtcc
    10801 ggtaataaga acggcttctc gcctgagaag ccgaaaaggc aacgccccgt catcgcggaa
    10861 cgctgcctga cacattattt ttcaatcgga tagatagtac ccgtattcat aatgccatta
    10921 ggatcgaact gctttttcag cccttccagc aacgcccacg cgctgccgtg ttccagtttg
    10981 ctccagtgaa cgcgatgttt accgataccg tggtggtgca ccatcgaacc gccgaggcga
    11041 atggtttctt cacagatgat cttgttgagc ggattgtggt acttgtcgat ttcctcttcc
    11101 ggcttacagt caacgacgtt gtaatcgtag acgaagtaca tgttggtgcc gttctgatag
    11161 ctatgagagg aatgaccgcc cagcatggtg atgtcgtcgg cgtgcgggaa ctcagtacga
    11221 atacggttaa taacgctttc gtagatttcg tggatgcagc tccagcagcc ggacacttca
    11281 gtggtaaagc ccatgttgcc ggttttgagg atctgcacac gttcggcagc cactttatcc
    11341 ggtccccagt tcaggttgtt aaaccaggtt tcgatcagtt tgctgtccac gcgctggcac
    11401 tgcgggtagc gggcaacgat ttccgcaatc ccttcgcccg tcgccttcgc aatgcgaggg
    11461 ttaccttcag ccataaagat cagcacacat tttccgtctg caaaatgggt gaagtgttgg
    11521 gtgccatctt cagcgtcata caaacgagcg atcgacggac gatacccttc caccatgact
    11581 tcacgcagga tgttgaagcc ggttttcatg tcttccagga tatagccgta gaagaggttg
    11641 ttttccgggg tgaatttaaa gattttcact gttacttcag tgatatagca aagtgcacct
    11701 tcgttaccga tgatgatgtg gcgaatgtcc gtgccagccg cgcggcgtgg cacgttttta
    11761 atgcgtgtga cggtgccatc agccaatact gcttccagac caacgaccat atcttcgatt
    11821 gcgccgtaga gtgtggagaa ctgcccgata ctgcgggttg ctaccaggcc gcccatctgc
    11881 gccagcggct ttgactgcgg agaatgcccc gtggtgtaac ctttttcacg caacgcattt
    11941 tccagcactt cctggggaac gccacattgc gccgtcgcct gcatattctc aatatcaata
    12001 ttaatgattt gattcatggc ggaaccgtcg agcaccaccg agttttctac aacagtttcc
    12061 agcccacctt cagtggcgga agcgccggta cgcggcacac cgttaatttt gtgcgcattc
    12121 ataaaattca gcacacggga gacttgttct gtggaaccga gttttacgac cgctgccgga
    12181 atcggcaaag tataaatacc atgaatatcc ggaaatttac gaaaacggtc aatactgttc
    12241 ttctttaata ctgtttcatc ggtaattacg cgatctgcac caacaatttc ctttagttgg
    12301 tcgacaatcg ctgcgcgaga taaagacata gtaattcctt cctgataata aaaatggaat
    12361 aatcattaat aagagaaaac agaaaccacc gttatggatt agcgcactaa ataaccacca
    12421 tcgaccacta ataaatgccc attgacataa tttgaagccg gacttgcgag gaatacagct
    12481 gcgcccatta aatcctgagt atcgccccaa cggtttgccg gaatatgatc aagaacgcgc
    12541 tgattggttt ctggattact gcgtgtcgcc agcgtaatat ctgttgcata atagccaggg
    12601 gcaataccat ttacctgaat attatattga cctagttcat cacaataagc tttggtgaat
    12661 ccggcaagag catgtttagt ggcagaatat gcaggtgacc attgtccacc taagtaagag
    12721 aacaatgaac agatattaat aattttgccg cttttctgcg ggatcataat ttttgcagct
    12781 tcatagctta actcgaatgc ggcggtcagg ttcacatcaa tcatcggatc ccagtcggca
    12841 cgaccgaagt ccagcacctt attcagctta caaataccgg cattgtttac cagaatatca
    12901 actgtaccga aacgctcaca gcaagcagcg ataatcttct gcggcgcgcc ttctgcggtg
    12961 atatccacct gcatgaagtc cacctcaaca ccctgttttt caatcatttc ctttgtttcg
    13021 ccgttatctt tgacgaaact aggaataaag atatttgcgc cagctttggc caacgccatg
    13081 gcaaatgcct agcctaaacc gctattccca ccggtaacaa ttgcggtttt acctttcagt
    13141 gagaaaaaat ccattgagaa cgcattgaga gattcgattg acatagttag ctccaatttc
    13201 cgtaaggcaa aaaaaagaaa ggtaacttcc cccgcgagag ggaagttacc tttctcatta
    13261 tctctggtaa ctggagataa tttttagcat aacggcataa ctgctaatgt gatcgtaatc
    13321 acagtgtgat attcgtattg tcaaatagtt ttaaaaagca gaagcgtgat tactctcaca
    13381 caacacctct gctttccatg ttatttctta ttgcaattac tagagacctg agaaaagtaa
    13441 tccacctgaa agggcggctt acttttctct tttttttgtc tattacaaat gggaatacga
    13501 tatgcaacac aactcatatc gccgttggat aaccctcgcg ataattagtt ttagcggcgg
    13561 cgttagtttt gacctggctt atttacgtta tatttatcaa attcccatgg cgaaatttat
    13621 gggattcagc aataccgaga taggtttaat aatgagtacc tttggtattg cggctattat
    13681 tctttatgcc cccagcggcg ttattgccga taaattttca caccgcaaaa tgattacttc
    13741 cgcgatgatc attaccggat tactgggtct gttaatggca acgtatccac cgctgtgggt
    13801 aatgctctgt attcaggtcg cctttgcgat aacgacgatt ttaatgctgt ggtcggtgtc
    13861 gattaaagcc gcctcgttgc tgggcgatca tagtgagcaa gggaaaatta tgggctggat
    13921 ggaagggctg cgcggcgtcg gtgtaatgtc gctggcggtg tttaccatgt gggtcttttc
    13981 tcgctttgca ccggatgaca gcgccagcct gaaaacagtc attatcatct acagtgtggt
    14041 ttacatcttg ttggggattc tgtgctggtt ttttgttagc gataacaaca acctgcgcag
    14101 tgccaataac gaagaaaaac agtcattcca gcttagcgac atccttgccg ttttgcgtat
    14161 cagcaccacc tggtattgca gcatggtgat ttttggtgtc ttcacaatct acgccattct
    14221 gagttactcc accaactatc tgaccgaaat gtacggcatg tcgctggtgg cagcgagcta
    14281 catggggatt gtgatcaaca aaatcttccg cgcgctgtgc ggcccacttg gcggcattat
    14341 caccacctac agtaaagtga aatcccctac ccgcgtgatc caaatccttt ccgtactcgg
    14401 cctgctggcg ttaactgccc tgctcgtcac gaactctaac ccgcaatcgg tcgcgatggg
    14461 gattggcctg attttactgc tgggattcac ctgttacgcc tcacgcgggc tgtactgggc
    14521 ctgccctggc gaagtgagaa caccgtctta cattatgggc accacggtag gtatttgttc
    14581 ggtgattgga ttcctgccgg atgtcttcgt ttacccgatt atcggccact ggcaagacac
    14641 tctgcccgca gcagaagcct accgcaatat gtggctgatg ggaatggcgg cgcttgccat
    14701 ggtgattgtc tttacctttt tgctgttcca aaaaattcgt actgctgata gcgcccccgc
    14761 aatggctagc agcaagtaag ccaggaaaat tacaggagaa aaatcatgtc gaaaaaatac
    14821 atcataggga ttgatggcgg aagtcagagc acaaaagtgg tgatgtacga tctggaaggt
    14881 agtgtggttt gcgaaggtaa aggcttatta cagccgatgc acacgcctta tgccgatact
    14941 gcagaacatc ctgacgacga tttatgggca tcattatgtt ttgcaggtca cgatttgatg
    15001 agccagtttg ccgggaataa agaagatatt gttggcatcg gcctgggctc catccgttgc
    15061 tgccgtgcgt tattgaaagc cgatggcacg cctgctgcgc cgttgattag ctggcaggat
    15121 gcacgcgtta cacgccctta cgaacatacg aatcctgacg tggcgtatgt cacctctttt
    15181 tcgggttatc tgacgcatcg cttaaccggc gagtttaaag acaatatcgc caactatttt
    15241 ggtcagtggc cggtggatta taagagctgg gcatggagcg aagatgctgc ggtaatggat
    15301 aagtttaata tcccccgtca tatgctgttt gatgtgcaaa tgcctggcac cgtcctcgga
    15361 catatcacac cacaagccgc actggcgaca catttcccgg caggactgcc ggttgtttgt
    15421 accaccagtg ataaaccggt agaagctctg ggggccggat tactggatga tgaaactgcg
    15481 gtaatttctc ttggcactta catcgcattg atgatgaacg gcaaagcact gccgaaagat
    15541 ccggtggcgt actggccgat tatgtcttct attccgcaaa cattgctgta tgaaggttac
    15601 ggtattcgca aaggtatgtg gacggtgagc tggctgcgtg acatgttagg cgagtcgtta
    15661 attcaggatg ccagggcgca ggatctttca ccggaagatt tactcaacaa gaaagcctct
    15721 agtgtgccac ctggctgtaa tgggctgatg acggtgctgg actggctgac caatccgtgg
    15781 gaaccgtaca aacgcgggat tatgatcggc tttgattcca gcatggatta cgcatggata
    15841 tatcgttcga tattggaaag cgtggcgcta acgctgaaga acaattacga caatatgtgt
    15901 aatgaaatga atcactttgc gaagcatgtg atcattactg gcggcggttc gaacagcgat
    15961 ctgtttatgc aaatttttgc cgacgtgttc aaccttccgg cacgccgtaa cgccattaac
    16021 ggttgtgcaa gtctgggagc agcgattaat acagcggtag gtctggggct atacccggat
    16081 tacgcaacgg ctgttgataa gatggttcgc gtgaaagata tctttatacc gattgagagc
    16141 aatgccaaac gctacgacgc gatgaataaa ggcattttca aagacctaac caaacatact
    16201 gatgtgatcc tgaaaaaatc gtatgaagtg atgcatgggg aattggggaa tgtggattcg
    16261 atccagagct ggtcgaatgc gtgaatgagg taggccgggc ggaaaactgc ccggcctgaa
    16321 catacctaaa tggttatccc cgctgacgcg gggaacataa gttcgacaac ttaaaaaatg
    16381 ataacaagtc cggtttatcc ccgctgatgc agggaacata ataaaaactt taactagttc
    16441 ggtttatccc tgtttttaca aggaatatta actacactcg atgtctttag aattaaagac
    16501 aatacgaatc gcactttatc aattttcact taaagtatca actggatatc tttattcata
    16561 agaactattc atcaacagcg tatagaggcg gttatgtaaa accactcatt agcctcaaaa
    16621 caaagacaaa cttccagatt tattacttaa ccaatgaata acaagcttaa aaaatatact
    16681 gttattctaa agaaa
//
LOCUS       DAZFWL020000100        16082 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFWL020000100 DAZFWL020000000
VERSION     DAZFWL020000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.100"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            26..382
                     /locus_tag="ACHKNP_RS17340"
                     /old_locus_tag="pgaptmp_003468"
     CDS             26..382
                     /locus_tag="ACHKNP_RS17340"
                     /old_locus_tag="pgaptmp_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4705577.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(433..1329)
                     /gene="yhaJ"
                     /locus_tag="ACHKNP_RS17345"
                     /old_locus_tag="pgaptmp_003469"
     CDS             complement(433..1329)
                     /gene="yhaJ"
                     /locus_tag="ACHKNP_RS17345"
                     /old_locus_tag="pgaptmp_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGU4705578.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1434..2135
                     /gene="yhaK"
                     /locus_tag="ACHKNP_RS17350"
                     /old_locus_tag="pgaptmp_003470"
     CDS             1434..2135
                     /gene="yhaK"
                     /locus_tag="ACHKNP_RS17350"
                     /old_locus_tag="pgaptmp_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HGU4705579.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPDAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2158..2322
                     /gene="yhaL"
                     /locus_tag="ACHKNP_RS17355"
                     /old_locus_tag="pgaptmp_003471"
     CDS             2158..2322
                     /gene="yhaL"
                     /locus_tag="ACHKNP_RS17355"
                     /old_locus_tag="pgaptmp_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HGU4705580.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2534..3844)
                     /gene="cyuA"
                     /locus_tag="ACHKNP_RS17360"
                     /old_locus_tag="pgaptmp_003472"
     CDS             complement(2534..3844)
                     /gene="cyuA"
                     /locus_tag="ACHKNP_RS17360"
                     /old_locus_tag="pgaptmp_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HGU4705581.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3872..5203)
                     /gene="cyuP"
                     /locus_tag="ACHKNP_RS17365"
                     /old_locus_tag="pgaptmp_003473"
     CDS             complement(3872..5203)
                     /gene="cyuP"
                     /locus_tag="ACHKNP_RS17365"
                     /old_locus_tag="pgaptmp_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4705582.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5478..6842)
                     /gene="tdcG"
                     /locus_tag="ACHKNP_RS17370"
                     /old_locus_tag="pgaptmp_003474"
     CDS             complement(5478..6842)
                     /gene="tdcG"
                     /locus_tag="ACHKNP_RS17370"
                     /old_locus_tag="pgaptmp_003474"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU4705583.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6914..7303)
                     /gene="tdcF"
                     /locus_tag="ACHKNP_RS17375"
                     /old_locus_tag="pgaptmp_003475"
     CDS             complement(6914..7303)
                     /gene="tdcF"
                     /locus_tag="ACHKNP_RS17375"
                     /old_locus_tag="pgaptmp_003475"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HGU4705584.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7317..9611)
                     /gene="tdcE"
                     /locus_tag="ACHKNP_RS17380"
                     /old_locus_tag="pgaptmp_003476"
     CDS             complement(7317..9611)
                     /gene="tdcE"
                     /locus_tag="ACHKNP_RS17380"
                     /old_locus_tag="pgaptmp_003476"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HGU4705585.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVTTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9645..10853)
                     /gene="tdcD"
                     /locus_tag="ACHKNP_RS17385"
                     /old_locus_tag="pgaptmp_003477"
     CDS             complement(9645..10853)
                     /gene="tdcD"
                     /locus_tag="ACHKNP_RS17385"
                     /old_locus_tag="pgaptmp_003477"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HGU4705586.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGSSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10879..12210)
                     /gene="tdcC"
                     /locus_tag="ACHKNP_RS17390"
                     /old_locus_tag="pgaptmp_003478"
     CDS             complement(10879..12210)
                     /gene="tdcC"
                     /locus_tag="ACHKNP_RS17390"
                     /old_locus_tag="pgaptmp_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HGU4705587.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12232..13221)
                     /gene="tdcB"
                     /locus_tag="ACHKNP_RS17395"
                     /old_locus_tag="pgaptmp_003479"
     CDS             complement(12232..13221)
                     /gene="tdcB"
                     /locus_tag="ACHKNP_RS17395"
                     /old_locus_tag="pgaptmp_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HGU4705588.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13320..14258)
                     /gene="tdcA"
                     /locus_tag="ACHKNP_RS17400"
                     /old_locus_tag="pgaptmp_003480"
     CDS             complement(13320..14258)
                     /gene="tdcA"
                     /locus_tag="ACHKNP_RS17400"
                     /old_locus_tag="pgaptmp_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HGU4705589.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAVV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14573..14791
                     /gene="tdcR"
                     /locus_tag="ACHKNP_RS17405"
                     /old_locus_tag="pgaptmp_003481"
     CDS             14573..14791
                     /gene="tdcR"
                     /locus_tag="ACHKNP_RS17405"
                     /old_locus_tag="pgaptmp_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HGU4705590.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            15047..15586
                     /gene="yhaB"
                     /locus_tag="ACHKNP_RS17410"
                     /old_locus_tag="pgaptmp_003482"
     CDS             15047..15586
                     /gene="yhaB"
                     /locus_tag="ACHKNP_RS17410"
                     /old_locus_tag="pgaptmp_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HGU4705591.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            15608..>16082
                     /locus_tag="ACHKNP_RS17415"
                     /old_locus_tag="pgaptmp_003483"
     CDS             15608..>16082
                     /locus_tag="ACHKNP_RS17415"
                     /old_locus_tag="pgaptmp_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705592.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDIPESPTGNNLEALAPGIEKPKQ
                     TSIEMVTLLNTLQPDGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQKISTGSINVNCPLKYIYNEIRRLNVIFSCGTGDIVDLSSLDL"
BASE COUNT         4300 a         4169 c         3653 g         3960 t
ORIGIN      
        1 cacttttaat taaagggatg tttttatgca gtggtattta gccgtattaa aaaattatgt
       61 tggtttctct ggtcgcgcac gtcgtaaaga gtactggatg tttactctga ttaacgccat
      121 cgtcggcgct attatcaatg tcattcaatt gattttaggt ttagagtttc cattcctttc
      181 tctcatttac ctggcagcaa caatcattcc ggtcattgct ctgtgtgttc gtcgtctgca
      241 cgatactgac cgttcaggtg cgtgggcgct tctctatctg gtaccgatca ttggctggtt
      301 agttctgttt gtatttgcct gtttagaagg caattctggc agcaatcgtt acggaaacga
      361 tcctaagttt ggttcaaatt aatcttagaa ttggggcgac atttcgcccc tttttattaa
      421 taataacaaa tattattttc cgctaaaaag tttgggaatt tcccgcagac accaggattt
      481 cgcttccccc atactgtcac gacgccaggc cataataata tcgatctcgc tggtcgattc
      541 cgggctgacg acacgcaacc gcccttccgc aatatctttt tcgaccatcg gatacggcat
      601 cgtcgccacg ccaagccccg ccagtaatgc ctgacgttta tcttcaatcg tgctcaccgt
      661 taagcgcggc tgtttgtcca gcagctgtac ggtcaacacc gggcgctcac gagcggtatc
      721 cgctaccgca attccacgat atttcacgcg cgtcacttca gataacggtt ccggctcctg
      781 atgaatcggg tgatcaggcg cggcaacgta gacgttcatt aacgtataga gcttgcgcga
      841 gttgatctcc gacgaggaac gaaaatgcat atccggcgcg ataacaatat ccgcccgccc
      901 ctgctccagt cgttcccacg cccccgccag cacttcggtg atgattgcca gttgggtatt
      961 ggcttttgcc gccagtttgt cgattaacgg gaaaaaggca ggtgtcggta ccagcgcttc
     1021 ggtcacaatg gtgagatgcg tttcccaacc gcgcgcgagg gcttccgcat cggtagtcag
     1081 tttatctgcg gcttccagca aaacgcgccc tcgctccagc aacatcctcc cgacattggt
     1141 gaatttggtg cgatggcccg agcggtcaaa cagcaccaca tccagctctt cttccagttt
     1201 ttgcatggtg tagctaagtg cagaaggcac gcgtcccagc tcatccgccg ccgccgcaaa
     1261 actgccccgg cgatcgatcg catccataac ccgtagtgct tccagcgtta atgccctttc
     1321 tttggccatt tcgttctcat tcaggaaatt tgaacatacc gggcagaata tctggctaac
     1381 aatgtagcgt ccagcccctt accataaaag gaagtaaaga gaggtcaaga attatgatta
     1441 ctacccgaac tgccaggcag tgtggacaag cagactacgg atggttgcag gcccggtata
     1501 ctttttcctt tggacactac ttcgacccga aattgttagg ctatgcctcc ctgcgtgtgc
     1561 ttaaccagga agtgctggcc ccagatgccg cctttcagcc gcgaacctat cccaaagtcg
     1621 atattttaaa tgtgattctg gatggagaag cagagtatcg cgacagcgaa ggcaatcatg
     1681 ttcaggccag cgccggtgag gcgttgctgc tctctaccca gccgggtgtc agctacagcg
     1741 aacacaatct cagcaaagac aaaccgttaa cacgaatgca gctttggctg gacgcctgcc
     1801 cgcagcgaga gaatccgctg attcaaaaac tggcgcttaa tatgggcaag cagcaattaa
     1861 tcgcctcgcc agagggtacg atgggaagcc tgcaattacg ccagcaagtg tggctgcacc
     1921 atatcgtgct cgacaaaggc gaaagtgcga atttccagtt gcatgggcca cgcgcgtatt
     1981 tgcaatcgat tcacgggaaa tttcatgcgc ttacgcatca tgaagagaaa gcggcgctga
     2041 cctgcggtga tggggcgttt attcgtgacg aggctaacat tacgctggtt gccgattccc
     2101 cactgcgcgc tttgctgata gatttgcctg tctagttgtt tttacaggga gatgatgatg
     2161 agtaaaaaat cggccaaaaa gcgccagcca gtgaagcccg tggtggcgaa agaacctgct
     2221 cgcaccgcca aaaattttgg ctatgaagag atgttgagcg agctggaagc catcgtcgcg
     2281 gatgctgaaa cgcgtttagc cgaggatgaa gctaccgcgt aaagacaggt tgccggatgc
     2341 ggcgaaaacg ccttatccgg cctacaaaat ccaacaacat taacgaatta aacaacccgt
     2401 aggccggata agatgcgcca gcatcgcatc cggcatcagg tacaaaacac aacaacatca
     2461 acaagttgag caccccgtag gccggataag gtgcgccagc atcgcatccg gcacgatacc
     2521 caaaacctgg cgtttatctg gccttgctcg ccataatctc gataatctgc cgatccgttt
     2581 gctgcatcga atggcttgct aacgcacaca ggttggcaat cgactgctca acatcatgcg
     2641 ccacgatccc ttcattgccg gtcacggcgg tatcatccag cgccattaac accgctttcc
     2701 acgcagccga agcactggtc gaaaccttca tcgcgcagct gttcgacgca ccatcgcaaa
     2761 tcatgccgct gacatcgccg atcatactgc tgatcgccat cgagatggtt tcataacgcc
     2821 catccaccag ccatgccatc ccggcggcgg cccccattgc tgcggtcgtt gcggcacaca
     2881 gcgcagacaa acgcggtaac tggttatgga tgtaaattgc gctcaaatga gaaagcatca
     2941 gcgcacgcgc cagccgttca tcatccgctc cgaagtgttc tgctaccacc accacaggca
     3001 ttgttgcggt gatcccctgg ttacccgagc cggagttact catagccgga agcgtagcgc
     3061 cgcccatacg cgcatcggat gccgcgctgg tacgaatcac aatggatgaa gagagatctt
     3121 tcgccagcaa accgcgctcg cactgttttt ccagcgtcgc gccaatatgc agcccccact
     3181 taccgctcaa accttcctgc gataacgcac aatttagctt cgcggaatcg agaataaagc
     3241 ggatcgccgc aaacgggact tcattgacga acttcaggat ctcagccagc gtcgttctgg
     3301 aaagcaccgt cagcggagac tcttgctcgc cctctgccac acacgcctgc tgggtaaaca
     3361 ccacaccatt gtgcgtctca atatgcacaa tgttggtatg cccgccgacg atggtgacac
     3421 acgcccactt ctcaccgttc cagactttgg cgcgtgagaa gaggatttca ttgcaaggtt
     3481 cctggatctt aacggagact ttccccgccg ccagcagtgc tttggcatcg gcaattgcct
     3541 gcgcagttgc gtctttcagc acttccagcc cggcgttggc atttccacct aacgccccca
     3601 gcgccgccgc aatcggcagc cccaccattc ccgtgccggg aacggtgacg cccagaccgt
     3661 tcttcatcag atttggcgaa acccaggctt ctacacgttc aaccggacct tccagttctg
     3721 ccgcagcaac cgccgccgcc agcgccagtg aaatcggttc agtacatccc agcgccggtt
     3781 ttacttcctc ctgaacggcg aggatgtaac gctgccataa cggatttaaa gtcgaatcaa
     3841 acatattaaa aaccttaaaa tttcaggtaa atcaagaaaa tgccaggaac ggagaaacac
     3901 aaagcaacaa accagtgacg ataatcaggt acagagacat ccctttgtat ttgtgcaatg
     3961 ccggtacttt gtaaaccagc cacgccggga tcaggcaccc taccatgccg aaaatcgggc
     4021 tacagataga ggtgaagctc aacaccggtg cgttcagtac gatggcgctc caggccagca
     4081 aaatggcgaa aatcatgatg ccgcgctgaa caagattttc gttaatcttc tcggcaggca
     4141 tcttgcgacg caggatgttc attacgatcc cttgcgttgc ttcgcgaaag cctaaataga
     4201 cgccaaagaa tgcagtcatg acagcaaaga tattgagaat gacgctgacc actttcaccc
     4261 acgctgcgcc gtcaccgcta ataaactgtg cggcaatcgc cagcgcggaa atattctgct
     4321 cataggcttt taccgcttcg tcatgtccca tcgccagcgt gaacgacacg gcgtagaaaa
     4381 agacggtgac aaacaaaatg ccaaacgcga tattcattgc ccgcaatgct ttatgccgcg
     4441 ccacttcaat tgatttttcc cgcgagcgat aagagatcac catcgggctt aacgtctgga
     4501 taaacagaat cgacgtcagg gtaaacggca gcgtaataat ggcgtttttc accagcagcc
     4561 ccagcggcgg tagcgaaccg acgttataca gatgccacat tcccaccatc gacacgccca
     4621 gcgccgcgac caccagcagc ttggtcagca ccatgccggt cgaaattttg aataacaatt
     4681 tctcgccgcg tgaagagatc gccaccagaa tgcaaatcag taccagacca taaaagggac
     4741 tgtctgacag caacccttcc gtcacgccga aggtatgcag ataggaagca ctatcgttgg
     4801 tgatggcggt ggaatagacg aacatccaaa tcaccagcat tacgaaatag agcgcaccta
     4861 acaggatgcc ccagttttta cctaaataac cgctaatgac actcgggtaa tctttacatt
     4921 ctggtgattc tgccagcgta ttaataaaca accgctgaaa cagatacatt gccgggtaac
     4981 caatcaccga tgagagcaaa aatacccaca atcccatcaa accgacctgc accgggagaa
     5041 aaacaatccc cgcgccaatc gccatcccga tactcataat cacccagccg gtgtcagtac
     5101 tgtcgaattt gatcgcttct cgccactcgc tctcactcat tccagcacga cccgccgggg
     5161 tcgaagcgtc tgcaatgacg cctttattcg atgcaatttc cataatttct cgctcaatat
     5221 tttgtagggc ttattatttt tttccgagcc gcatcaaggc gatatgcggt ctacgttttt
     5281 gcaggcgagt tattagaata aagaaatgat acgcgcgagg tcagagaaaa tcttcacaat
     5341 caagactttg aaataacgga gatggataag aattttctac taaattaatc gcaagagaat
     5401 attcattctc tatttgcggc agatcacaaa aaaaaggtgc acatttgtgc acccaaggat
     5461 gaaagctgac agcaatgtca gccgcagacc actttaatgg ccagtcctcc gcgtgatgtt
     5521 tcgcggtatt tatcgttcat atctttgccg gtttcataca tcgtctcgat cactttatcg
     5581 agtgaaacac gcggtgccga ggtgcggcgc atcgccatcc gcgcggcgtt tactgctttc
     5641 acggcattaa tggcattacg ttcaatgcac gggatttgta cctgtccggc aaccggatcg
     5701 caggtcagcc caaggttatg ctccatcgcg atttccgccg cattgcatac ctgcgccgga
     5761 ctgccgccca gtagttcagt taaccctgcc gccgccattg aacaggccac gccaatctcc
     5821 ccctgacagc cgacctccgc gccagagatg gaggcgttca ttttatacag cgcgccaata
     5881 gcccccgctg ccagaaaata gcgggcaatt gaccgctcgt ttaccggacg acggaactta
     5941 tcgtaataag ccagcactgc cggaataatg ccgcacgcac cgttagtcgg tgccgttacc
     6001 acgcgcccgc cagctgcgtt ttcttcacta accgccagtg cgtacatgtt gatccagtcg
     6061 atgacattca tcggatcgtt agagatgtta tcgctggaaa ccagctgacg acgcagcgct
     6121 acggcacgac gcggcacatt gagcggacca ggcagcacgc cttcagtgtt catcccgcgt
     6181 tcaataccgt catgcatcac ttgccagata cgggcaaacc ctgcgtcaat ttccgctttg
     6241 ctgcgcagcg ccagctcgtt gtgcatcatc aggccagata tagacaggcc gttgtaatca
     6301 cacattttca gcagttcacc tgctgagtgg aaatcgtaag gtacggacgt ttcgacatcg
     6361 tgcgacaggc cgaagtgttc ttcttcgaca ataaacccgc cgccgacaga gtaataggtt
     6421 ttacttaata gcgcttcctg tcccttccag gcagtgatcc gcattccgtt ctcatggcga
     6481 ggcaacattt cgggatggaa gataatgttc tttgctacag gaaaatcaac aatatgcgca
     6541 ccagatgcca ctggcagccg cccgctgcgc gttactaact ctataaatgc agggatctca
     6601 tcaatgacaa catcctgcgg actgtttcct gccagtccca tgataatggc gacatccgtg
     6661 gcatggcctt tgcccgtcag tgacaacgac ccatacagat cgaccacaat atggctcgtc
     6721 gcggttaata agccgctact ttccagccga tcaataaaac tttttccggc attcattggc
     6781 cccacggtat gcgaactgga gggaccaatc ccaattttga aaatatcgaa tgcactaatc
     6841 atatccacac cctcggattg ccgttcagtg aagtggagcg gaacgacctt gcgaccgtcc
     6901 cgctcacgag gctttacgca ctacgtactg cgatagcttc aatttccagc ttcacatctt
     6961 tcggcaaacg cgcaacctgc acacagctcc gggtcgggta ggtcgcctga tgctcatcga
     7021 agaactgctt atagacttca ttgatggtgg caaaatcatt cagatcggtg atgaacacgg
     7081 tcatcttgat gatatcgccc acgctcagcc cggcagcaac cacgatcgct ttgacgtttt
     7141 cgaggcttaa acgcgcctga tcttgcacat cagccgggat ctcaccggtc tgcgggcaaa
     7201 ccggaatttg cccggaggtg aagaccatgc tgcctaaatc aacgccctga acataagggc
     7261 cgattgcgcc tggggcacgt tgcgtttcga taatcttttt catacatcct ccggcgtcag
     7321 agcgcctggg taaaagtacg tgaaataaca tcctgttgct gttcacgggt cagtgcgttg
     7381 aagcgcacgg cgtagccaga gacacggatt gtcaggttag gatatttttc cgggtgctcg
     7441 atggcatcca gcagcatttc ccgattcatt acgttgacgt tgaggtgttg accgccttcg
     7501 acatccgctt cgtggtggaa atacccatcc agcaggccga caaggttggt tttacgtact
     7561 ggatcttctt tgcccagcgc cgcaggaacg attgagaagg tgtacgagat cccatctttg
     7621 gcgtaggtga acggcagttt cgccaccgac gtcaatgagg ccacggcacc tttgcggtca
     7681 cgaccatgca tcgggttagc gcccggcgcg aacggtgttc cggcgcgacg accgtccggc
     7741 gtattaccgg ttttctggcc gtacaccacg ttagaagtga tagtcagaat cgactgggta
     7801 gtgacggcgt tgcgataggt tggcagcgct ttaattttct tcataaagcg ttcaaccagg
     7861 tcgcaggcaa tgctgtctac gcgctcgtcg ttgttgccgt actgcggata ttcaccgtcg
     7921 atttcaaagt ccaccgccag gccgttttcg tcacggattg gtttcacgcg ggcatatttg
     7981 atggcagaca gggagtccgt cgccaccgac aggcccgcga tgccgcatgc catagtgcga
     8041 tagacatcac gatcgtgcag cgccatcagc gaagcttcgt agctgtactt gtcgtgcatg
     8101 tagtgaatga tattcagcgc gctgatgtac tgcaccgcca gccagtccat gaagtgatcg
     8161 aggctgtcca tcactttgtc gtaatccagc acgtcgtcca tcagcggtgc tgttttcggc
     8221 ccgacctgaa tcttcagctt ctcgtccacc ccgccgttaa ttgcgtagag cagcgttttc
     8281 gccaggttag cgcgtgcacc aaagaactgc atttgcttac caatcaccat tgggctgacg
     8341 cagcaggcaa tcgcgtaatc gtcgctgttg aagtcagtac gcatcagatc gtcattttca
     8401 tactgcaagg aagaggtgac gatcgacacc tgcgcggcat attttttgaa ggcaatcggt
     8461 aattcttccg accaaagaat ggtcaggtta ggttccggtg ccggccccat agtgtgcagg
     8521 gtgtgcaaat agcggaagga gtttttggtc accagcgtac gaccgtccag ccccatcccg
     8581 ccgatcactt ccgtcgccca gattgggtcg ccggagaaca gcgaatcaaa ttccggtgta
     8641 cgcaggaagc gtaccatacg gatcttcatg atgaagtgat cgatcagttc ctgtgcctgc
     8701 tgctcattga gtacgccagc tttaaagtcg cgctcaatgt agatatcgag gaacgatgcc
     8761 gtgcggccca gcgacatcgc gccgccattt tgcgatttca ctgccgccag ataagcgaag
     8821 tagagccact gcaccgcttc ctgcgcattc tgcgccgggc gagagatatc aaagccatat
     8881 ttcgccgcca tttcctgaat ctgcaacagc gcatgacgat gctctgccag ctcctcacgc
     8941 agacggatgg tggcttccag atcctcgcct ttttccagac gagactggag atcggcaaat
     9001 tgcagttcgc gttcacgtac cagataactg atgccataca gcgctacgcg gcgatagtca
     9061 ccgataatgc gcccacggcc atagccatct ggtaaaccgg tcagcacgcc agatttacgg
     9121 cagcgcagca tatccggtga gtaaacatca aatacgccct ggttatgggt tttacgcaga
     9181 tcggtaaaca gatattcaaa ttcactgtcc atttcacggc cataggcgtg gaatgaactt
     9241 ttaatcatat taatgccacc gaacgggtgt agcgcacgtt tcaacggcgc atccgtttgc
     9301 aggccaacaa ttttttccag cggctggtta atatatcccg catcatgagc ggtaattgtg
     9361 gtggcaatat tggtatcgaa atcaaccggc gcgtgggttg cgttttcgat acggatacct
     9421 tccattactt tttcccacaa ttccgtggtg gcaggcgtcg cttcggcgag gaaagattca
     9481 tcgccttcat acggtgtata gttatgttga ataaaatcgc ggacattaat ttcgtttttc
     9541 cagtccgtac ctttaaagcc aagccatgcg tcggcgtaca gcttatcgct ggtatcaata
     9601 tctaccttca tgaaaaataa tctctctaca atacttcaac taaattatgc aaattctgcg
     9661 ggcgcgttaa ctttgcctaa atgaatggca tccaaagcaa tcattttttc ttcgttagtc
     9721 ggaataacag cgcaaatgac acgcgcattt tcactggaaa caattcgctc accacaggag
     9781 ttagagcgat tattcatttc tgtatcaatc tctacgccta atacagccaa atgttccatg
     9841 accagacgac gaattaagct tgaattctct cctattccgc cggtgaatat aattccatcc
     9901 aggcgacgta atgaagctgc gtgtccggca atatgacggg caattcggtg aacaaaggtt
     9961 ttaattgcca gttgcgcgcg ttcgtgacct tcatgccagg ctttttccag aacacgtaaa
    10021 tccgaagaaa gaccggaaat acctaataat cccgactctt tattcactac gcgttccagg
    10081 tcacccaggc tctggttggt ttggctggcg acccaggaca tcgcaccaaa gtcgacatcg
    10141 ccactgcggg tacccatcat caagccttcc agcggcgtca ttcccattga agtatcaaca
    10201 ctctgaccgt tgcgaaccgc gcagattgac gcgccattgc caagatgcgc cacaaccagg
    10261 ccggagtcat cttccgccag attcagcagc gaatgtgcgc gctgggaaac atagcggtgc
    10321 gacgagccgt ggaaaccata acggcgtaca cctaactctt cataatattt ccacggcagg
    10381 ccgtataaat aagcttccgg agccatcgtc tggtggaaac tggtatcaaa taccgccacc
    10441 tgagttacgc ccggaaataa ttgctgcgcc gattcaatac cacttaaatt ggcgtaatta
    10501 tgcaggggtg ccagtggaga aacgcgacgg atattatcaa tgacttcatc ggtaataatg
    10561 gcggactcgg taaaaatact gccgccgtga gcgatgcggt ggccaattaa ggccacactg
    10621 tcatttaaat tccgtttttc cagttcaaat gcaattgcct tcaatgcacc ttcgtagctg
    10681 tggtgagcca gcggtgctgg ctctccccca tttacggata agaatgcatt ttccgagtta
    10741 ataccgtcgg caatacctga cattaatact tcacagtcgc tggcatcgag tacggaaaac
    10801 ttaatcgaag acgaaccaca gttaataacc aaaacaaccg gaaattcatt catctctttt
    10861 ctcatcctga gttacggatt aaaacagttt gtatacgatg ttcaggatgg tcagcagacc
    10921 aatcacggta acaaacacgt tatccagacg accacggtat ttcgccagag acggcgcttt
    10981 acggatggca tacatcggca acaggcacag cagggatgcg ataatcggtg cgcccatggc
    11041 ttcaataagg tcgaggatgt tcgggttggc gtaggcaaca acccaggtgg agcccatgat
    11101 gaagatcatg ctgatagtgt tcagtttacc cagcgacact ttagttttgt cgcctttata
    11161 accgaacttc agaatcagac cattcaagcc ttccagcgtc cccagatagt gaccgaagaa
    11221 agatttgaag atagccacga gtgcgatgat ggaagccgca tattccagtg taatcgcgaa
    11281 cgttgttttg gtaccggtca tggacgcaaa gtggttagcc agataagaaa gcactggaat
    11341 attctgcgct ttggcttccg ccatgttggc cggagacagc gtaaacaggc agctaaaggc
    11401 aaagaacatc accactgcaa ccatcagcat gctggcacga gaaatgattt gggaacattt
    11461 acgttcggtg aagtcgcgac cgaagtcttt ctcatactct tcacgtttag aaaccacgaa
    11521 ggaagagacg attggcgaga agttaaagga gaaaaccatg atggaaatcc ccagccagac
    11581 agtgatcagg ataccgtcat gaccggttaa cgacagcgaa ccgaggtcaa cctggtcgat
    11641 aactgcagag ttccagtaag ggatcagcga cagagaaatc agcaccaggc tggcgataaa
    11701 cggccatacc aggtagctca tcactttaac catcagatcc ttaccaaacc agatgacgaa
    11761 agccatcagc agcaacagga acagcgccac aaagccgcga ttcagcggtg caaatccgag
    11821 ctggttttcc cagaacgtca taaaggtatt ggtaatagta acgccataaa tccacagcag
    11881 tgggcaaatc gcgaagaagt acaggaacgt gataaccacg ccgccagttt taccaaaatg
    11941 ctcttccacc gtttccgtaa tgttgccgga agggttagag ccagaaagac acagacgcgc
    12001 cagcgcccgg tggcaataaa atgcgatggg gtatgccaat accaacatca gaagaatcgg
    12061 gatcagtccg ccaaaacctg cgcggatagg gaagaacagc accccggcgc cgattgccgt
    12121 accaaacaag cctaacgtcc atgtggtatc tgatttacgc caggacgatt gttttgtctg
    12181 gctggataca atgctatctg aagtactcat atcctatcct caacgaatta attaagcgtc
    12241 aacgaaaccg gtgatttgag agacgcgaga aagatcgata ttgccgccgg aaataatact
    12301 gacggttttt ctgttttgaa tatattggtc taatttaccg cttaataatg cagcacatgc
    12361 cagagcgcct gcgccttcgg tgacgacttt attgcgctga attaaggcaa tcatactgtt
    12421 tctgatttcg tcttcgctga ccagcacgat gtcatcgact aattcacgaa cgatttcgta
    12481 agttaaatta cccgggcggg agacatcaca accatccgcc agggtgccgg tagttcggtg
    12541 cgtggttatt tctccggagt ggaaagaagc cgccatgccg tgaacgtttt cagactgtac
    12601 gccaataaca cgaatggtcg ggttaataga tttaattgcc accgcaatac cagcaattaa
    12661 accgccacca ccaattggca caatcacgtt atcgacatca tagagatctt ccataatttc
    12721 cagaccaatc gttccctggc cagcaatcac tttcggatca tcgtaaggtg ggataaaaat
    12781 acggccttcc atttcgacaa tttcgctcac tttagcgata gtgtcgttga aattatcacc
    12841 atgcagaacg acttctgcgg agtagtcgca cgttgccgct actttggatt ttggcgcacc
    12901 ttttggcatc accactttac cgtcgatacc cagcatcgcg caggagagcg aaaccccttg
    12961 cgcatggttg cccgcagaac aggccactac acctttacgt ttttccgcat cagtcagtga
    13021 acttaattta ttaaatgcgc cacgaatttt aaatgaaccc gtacgctgca tattttcaaa
    13081 cttcaggaat atttcacctt tgcaacgttc actaaaatag tttgagcgag gcatgcctgt
    13141 tttataaatt cgcccagcca gtcgttgttt cgcttcaata atgtcatcaa tagcaaccgg
    13201 cagatcgtat gtaatatgca ttataaaacc tcttcgcctg aattaaatat gtaggtaacc
    13261 gtaaccgaca cctgcaaaac agacaggtgg attatttatg tttaaataaa aacaaataac
    13321 taaccaactt ctattaattg ccttcgtcta cacccattat aagatgaata ctctttggct
    13381 aattccacca aaaccgatgc tgctttttta atacgataat ttttcgacca taccacggca
    13441 tattgtgcca caggtaatgt ttcttcaacc ggaatagtaa taaattgatt tgaaccaaaa
    13501 ggtgacgtca tatcacaagg aattacagtt aagaaatcag cattgagaac aagattataa
    13561 attgtcacga ctgagtcggt tttaacgatg ttttcaatac tgatgccatt tctttgtaac
    13621 gtagtaagaa gctcgctgta gtaccccata ttggtttgtg gcaacaccca ctgttcgttc
    13681 ttcaacgact ccagcgtggt ggtgccggtg catgttcggg acttactggc taccagcaca
    13741 aactcggact cgaacagcgg ctcaacatgt aaatcctgaa gcttcatttc tgcacttaac
    13801 gtaccaatcg caaaatccag tcgaccgtcg cggattgccg gtaagaacga agacagttgc
    13861 gcttcataca tagaaacctg cgctttcggg aacacctctt tgaacttgtt gatcatccct
    13921 gacataaaag taaaaccaat caatgaagga aaaccaaatg agacttccac caccgcctca
    13981 gaagacatac cgcttatctc attaaccata tttttcattt cacgggtaat ggattcggaa
    14041 cgggagagta acaattgacc ggcaggtgtt aatgttacac cggtattttt ccgcaccact
    14101 aattccacac caaaataatc ttcaatatcg ttaatgattt tactgacggc cggttgagtt
    14161 aaccctaatt cttttgcagc cgagccgata gagccacttc taatgacttc ctgaaagact
    14221 accaggtgct gcgttttcgg aagaagaata gtgctcataa cgacctacgt taattacctc
    14281 attgacggca tgaagtgtat caaaatgaaa tgaacaggat atgtgcgacc actcacaaat
    14341 taactttcaa tactttccat ggtatcgtta cgcaaattaa atacctttaa atatcaacaa
    14401 gttaaagtat aaaaatcagc ataaaccctg atttttgtca aaaaaaatga cggggataac
    14461 catattttat ggcgataaca tcattcgtta tgtggttaat ataaaaaagg ggctgagacc
    14521 atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt taatgagcaa
    14581 gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg cacgtcacat
    14641 tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta aaatttcgaa
    14701 tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt ctgacaggac
    14761 gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg cgacgaatag
    14821 cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa ggtcagacaa
    14881 agacaacaag ggaaattttc acagagcttt tgatcgtcgt aggccacaga atgttgcttc
    14941 gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgaaagaac cctgcgagga
    15001 aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga aaggcttccc
    15061 aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta acaatcacaa
    15121 tagaaatatt gattattgga cggtaaaaag aaagtttgca gaaattgtct ccaccaatga
    15181 cgttaataaa atttacagta taagtaatga actgcggaga gtattatctg caattactgc
    15241 attgaatttc tatcaaggcg atgttccttc tgtaatgatc cgaatccaac cggaaaatat
    15301 gagtccattc atcatagata tttctacagg ggaacatgat gattatatca tacaaacatt
    15361 agatgtaggc acttttgcac cttttggcga acaatgtact tgctcagccg tcaataaaaa
    15421 agagctggaa tgtattaagg agacgatttc taagtattgt gcaaaattca cccgaaaaga
    15481 agccatctta acaccccttg tgcactttaa taaaaccagc attacttcag attgttggca
    15541 aattctcttt ttttcacccg atcattttaa taatgatttt tattgattat ttataaagga
    15601 taaaataatg ttccctgttt catcaattgg taatgatatt agtagtgatt tagtccgtcg
    15661 aaaaatgaat gacattcctg aaagcccaac aggaaataac ctcgaagcac tagcaccagg
    15721 tatagaaaaa ccaaaacaga cctctattga aatggtcact ttacttaaca cgttacaacc
    15781 tgatggaaaa tgcattatca ctggtgattt tcaaaaagaa ttagcgtact tacaaaatgt
    15841 aattctttat aatgactcgt ctcttcgtct ggatttttta ggttataacg cccaaattat
    15901 tcaacgatcg gacaatacct gcgaacttac cattaatgaa ccattaaaga accagaaaat
    15961 atccacaggc agtatcaatg ttaattgtcc attgaaatat atttacaatg aaatcaggag
    16021 gttgaacgta atctttagtt gtggaactgg agatatcgtt gatctatcct ctctggactt
    16081 ac
//
LOCUS       DAZFWL020000101        16006 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFWL020000101 DAZFWL020000000
VERSION     DAZFWL020000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.101"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            548..1372
                     /locus_tag="ACHKNP_RS17420"
                     /old_locus_tag="pgaptmp_003484"
     CDS             548..1372
                     /locus_tag="ACHKNP_RS17420"
                     /old_locus_tag="pgaptmp_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HGU4705593.1"
                     /translation="MIKPFSVRYGHVDVREHVQLNDLNSDTRMALWNCLYLFLWTNNR
                     QTATATKCAQSVWIYYLNQPADNIPRYESGYKSDKTLLTAIRDYIYGEAWYLVYDLIE
                     FIIERTNSYINLSKHLNSIFKKHGVGYTIINGCITPISNDNEIESVQNAVDNGTDSSR
                     SHFERALQLMTDREQPDYRNSIKESISAIESLCRKITGNDKGTLGACLKAIEEKGYIH
                     SAMKGAFSQLYGYTSDQGGIRHALTEEDVNPTLAEAKFMLVTCSAFSNYLLSKISD"
     gene            complement(1421..1993)
                     /locus_tag="ACHKNP_RS17425"
                     /old_locus_tag="pgaptmp_003485"
     CDS             complement(1421..1993)
                     /locus_tag="ACHKNP_RS17425"
                     /old_locus_tag="pgaptmp_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705594.1"
                     /translation="MVDASAQPHIVVTLEPNPWAAFYFDINIANTGNAPAYNVEVVFD
                     PPLVNAEHREKSEIPFSKVSVLKNGQSLTSNLCKYEQIKDQIYNINISWASKPKSNDR
                     ETNEYVYDMATFEGISYLGARSPLTQIAEQIKGIREDWKPIAQGAKKVKADVYTSSDR
                     NEERTYLQEQHDLAIKRRDEKREKRLESGE"
     gene            2094..3264
                     /locus_tag="ACHKNP_RS17430"
                     /old_locus_tag="pgaptmp_003486"
                     /pseudo
     CDS             2094..3264
                     /locus_tag="ACHKNP_RS17430"
                     /old_locus_tag="pgaptmp_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3347..3604
                     /locus_tag="ACHKNP_RS17435"
                     /old_locus_tag="pgaptmp_003487"
     CDS             3347..3604
                     /locus_tag="ACHKNP_RS17435"
                     /old_locus_tag="pgaptmp_003487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705595.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHKDIIDNNRSINWPV"
     gene            complement(4162..4929)
                     /gene="fecE"
                     /locus_tag="ACHKNP_RS17440"
                     /old_locus_tag="pgaptmp_003488"
     CDS             complement(4162..4929)
                     /gene="fecE"
                     /locus_tag="ACHKNP_RS17440"
                     /old_locus_tag="pgaptmp_003488"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HGU4705596.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(4930..5886)
                     /gene="fecD"
                     /locus_tag="ACHKNP_RS17445"
                     /old_locus_tag="pgaptmp_003489"
     CDS             complement(4930..5886)
                     /gene="fecD"
                     /locus_tag="ACHKNP_RS17445"
                     /old_locus_tag="pgaptmp_003489"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HGU4705597.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(5883..6881)
                     /gene="fecC"
                     /locus_tag="ACHKNP_RS17450"
                     /old_locus_tag="pgaptmp_003490"
     CDS             complement(5883..6881)
                     /gene="fecC"
                     /locus_tag="ACHKNP_RS17450"
                     /old_locus_tag="pgaptmp_003490"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HGU4705598.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILASIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(6878..7780)
                     /gene="fecB"
                     /locus_tag="ACHKNP_RS17455"
                     /old_locus_tag="pgaptmp_003491"
     CDS             complement(6878..7780)
                     /gene="fecB"
                     /locus_tag="ACHKNP_RS17455"
                     /old_locus_tag="pgaptmp_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HGU4705599.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(7825..10149)
                     /gene="fecA"
                     /locus_tag="ACHKNP_RS17460"
                     /old_locus_tag="pgaptmp_003492"
     CDS             complement(7825..10149)
                     /gene="fecA"
                     /locus_tag="ACHKNP_RS17460"
                     /old_locus_tag="pgaptmp_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HGU4705600.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(10236..11189)
                     /gene="fecR"
                     /locus_tag="ACHKNP_RS17465"
                     /old_locus_tag="pgaptmp_003493"
     CDS             complement(10236..11189)
                     /gene="fecR"
                     /locus_tag="ACHKNP_RS17465"
                     /old_locus_tag="pgaptmp_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HGU4705601.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(11186..11707)
                     /gene="fecI"
                     /locus_tag="ACHKNP_RS17470"
                     /old_locus_tag="pgaptmp_003494"
     CDS             complement(11186..11707)
                     /gene="fecI"
                     /locus_tag="ACHKNP_RS17470"
                     /old_locus_tag="pgaptmp_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HGU4705602.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            11844..11912
                     /gene="ykiE"
                     /locus_tag="ACHKNP_RS17475"
                     /old_locus_tag="pgaptmp_003495"
     CDS             11844..11912
                     /gene="ykiE"
                     /locus_tag="ACHKNP_RS17475"
                     /old_locus_tag="pgaptmp_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HGU4705603.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            11999..12696
                     /locus_tag="ACHKNP_RS17480"
                     /old_locus_tag="pgaptmp_003496"
                     /pseudo
     CDS             11999..12696
                     /locus_tag="ACHKNP_RS17480"
                     /old_locus_tag="pgaptmp_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
     gene            complement(12707..12778)
                     /locus_tag="ACHKNP_RS17485"
                     /old_locus_tag="pgaptmp_003497"
                     /pseudo
     CDS             complement(12707..12778)
                     /locus_tag="ACHKNP_RS17485"
                     /old_locus_tag="pgaptmp_003497"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12841..12987
                     /locus_tag="ACHKNP_RS17490"
                     /old_locus_tag="pgaptmp_003498"
                     /pseudo
     CDS             12841..12987
                     /locus_tag="ACHKNP_RS17490"
                     /old_locus_tag="pgaptmp_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            13014..13118
                     /locus_tag="ACHKNP_RS17495"
                     /old_locus_tag="pgaptmp_003499"
                     /pseudo
     CDS             13014..13118
                     /locus_tag="ACHKNP_RS17495"
                     /old_locus_tag="pgaptmp_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            13422..13715
                     /locus_tag="ACHKNP_RS17500"
                     /old_locus_tag="pgaptmp_003500"
     CDS             13422..13715
                     /locus_tag="ACHKNP_RS17500"
                     /old_locus_tag="pgaptmp_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HGU4705604.1"
                     /translation="MVKLLTEPWGRKMHSDYYNMVFGEKLANILYEANSQFFYERNVI
                     EEAVNALFCEREIINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(14035..14166)
                     /locus_tag="ACHKNP_RS17505"
                     /old_locus_tag="pgaptmp_003501"
                     /pseudo
     CDS             complement(14035..14166)
                     /locus_tag="ACHKNP_RS17505"
                     /old_locus_tag="pgaptmp_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14448..15806
                     /locus_tag="ACHKNP_RS17510"
                     /old_locus_tag="pgaptmp_003502"
     CDS             14448..15806
                     /locus_tag="ACHKNP_RS17510"
                     /old_locus_tag="pgaptmp_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HGU4705605.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
BASE COUNT         3929 a         4045 c         4207 g         3825 t
ORIGIN      
        1 cggccttcgc accaagttac taaattcctt acaatacagt tagttagacg attaaatctg
       61 tctcttttgc ctgtaagaaa tgctctgtta tgtcccttga aaaagccgct gtggttgata
      121 gtggtaattg ggatgttaac tcatagcggc gtgtcgttcc aagcgtcaaa cacgtactga
      181 ctcaacttga aagctatgac gtagccaccg tctctaaaca atcccaaaac ttttagtgca
      241 gcgcatttta gcccgatgcc cccattctgt cgccgtcgcg agacgccgca ggggataaac
      301 aggtttatgg tggtcacggt acttgcagac catatgcaag ctctgccagc ggttcggcct
      361 cgctcccctt gggtacatgg tcatcctgat aagcctcact aactgacata accctgatga
      421 agtcgcaaga ggctccgctt gttgtcttac caccaacggg gctttatgga ttctattgct
      481 taatatatga ttgtatcaaa cttagactgc ataatttaaa cgaacataac aatcaggcag
      541 gggaagaatg ataaagccat tttctgtgag gtatggtcat gttgacgtta gagagcatgt
      601 ccagctgaat gatcttaatt ctgatacacg catggcattg tggaattgct tatatctttt
      661 cctttggact aacaatcgtc aaactgctac agcaacaaaa tgtgcgcagt ctgtgtggat
      721 atattatcta aatcaacctg cggataatat tcctcgttat gaaagtggat acaaaagtga
      781 taagacgttg ctgactgcta tccgagatta tatatatggg gaagcatggt atttggtata
      841 tgatttaatt gagttcatca ttgagagaac taattcatat attaatctat cgaagcatct
      901 caattccatc tttaaaaagc atggcgttgg ttataccata ataaatggtt gtattactcc
      961 tatttcaaat gataacgaaa ttgaaagtgt tcagaacgca gttgataatg gcactgattc
     1021 atcgcgtagc catttcgaaa gggcgttaca actaatgacg gatagagagc agccagacta
     1081 cagaaactcg attaaagagt ctatctctgc aattgaatct ttatgcagga aaatcacggg
     1141 caatgataaa ggaaccttgg gggcttgtct taaagctata gaggaaaaag gatatataca
     1201 ctctgcaatg aaaggcgctt ttagtcagct ctatggttat acaagtgatc agggtggaat
     1261 acgtcatgcg ctaacagaag aagatgtaaa tcctacctta gctgaagcta aatttatgtt
     1321 agtcacttgc agtgcgttca gtaattatct gttgtcaaaa ataagtgatt aattacaggt
     1381 ttatagttac gagtgggtta gttacccact ccctttaaaa ttattcacca gactctaatc
     1441 ttttttctct tttctcatct ctccttttta ttgccaaatc gtgttgctct tgcagatacg
     1501 tgcgttcttc gtttctatcg cttgaagtat atacgtctgc ttttactttt ttagctcctt
     1561 gtgcaatagg tttccaatcc tctcttatac ctttaatttg ttctgcaatt tgcgtcaatg
     1621 ggcttctcgc tcctagataa cttattcctt caaatgtcgc catgtcatac acatattcat
     1681 ttgtttctct atcgtttgat ttaggtttgc ttgcccagct tatattaata ttataaattt
     1741 gatctttgat ttgttcatac ttacagagat tgctggtaag tgattgccca ttttttaaca
     1801 cgcttacctt actaaacgga atctcacttt tttctctatg ctccgcattt actagtggag
     1861 gatcaaacac aacctcaaca ttataggcag gtgcgttccc tgtattagca atgttaatat
     1921 caaagtaaaa agcggcccaa gggtttggct caagtgttac aactatatgg ggttgagctg
     1981 atgcgtcaac cattcttttt gtctcgtctg ctctgaagtg gtgaagtggt ttactgaatt
     2041 tggccacctg aacagaggtg atatgctcac ctcagaacaa cacaggtgct ccaatgaaaa
     2101 aaagaaattt tagcgcagag tttaaacgcg aatccgctca actggttgtt gaccagacat
     2161 acacggtggc agatgccgcc aaagctatgg atgttggcct ttccacaatg acaagatggg
     2221 tcaaacaact gcgtgatgag cgtcagggca aaacaccaaa agcctctccg ataacaccag
     2281 aacaaatcga aatacgtaag ctgaggaaaa agctacaacg cattgaaatg gagaatgaaa
     2341 tattaaaaaa ggctacaacc gcgctcttga tgtcagactc cctgaacagt tctcgataat
     2401 caggaaactc agagcgcatt atcctgtggt cacactctgc catgtgttcg gggttcatcg
     2461 cagcagctac agatactgga aaaaccgtcc tgaaaaacca gacggcagac gggctgtatt
     2521 acgcagccag gtacttgaac tgcatggcat cagccacggc tctgccggag caagaagcat
     2581 cgccacaatg gcaacccgga gaggctacca gatggggcac tggcttgctg gcagactcat
     2641 gaaagagctg gggctggtca gttgccagca gccgactcac cggtataagc gtggcggtca
     2701 tgagcacgtt gctatcccga atcatcttga gcgacagttc gccgtaacgg aaccaaatca
     2761 ggtgtggtgc ggtgatgtga cctatatctg gacgggtaag cgctgggcgt acctcgccgt
     2821 tgttctcgac ctgttcgcaa gaaaaccagt gggctgggcc atgtcgttct cgccggacag
     2881 caggcttacc atgaaagcac tggaaatggc atgggaaacc cgtggtaagc ccgtcggggt
     2941 gatgttccag gcgatcaagg cagtcattat acgagcaggc agttccggca gttactgtgg
     3001 cgataccgga tcaggcagag tatgagtcgg cgtggaaact gctgggataa cagcccaatg
     3061 gagcgcttct tcaggagtct gaagaacgaa tgggtgccag cgacgggcta tgtaagcttc
     3121 agcgatgcag ctcacgcaat aacggactat atcgttggat attacagcgc actaagaccg
     3181 cacgaatata atggtgggtt accaccaaac gaatcagaaa accgatactg gaaaaactct
     3241 aacgcggagg ccagttttag ttgaccacaa cagactacct gaagggagcc gcggtcgcct
     3301 agcagttgca gtagcaggag atcatccagc cgcagtacag gtcacgatga ctctggttaa
     3361 tgataccggc tttgaccccg tattttccgg ctctatcgct gaatcatggc gtcagcagcc
     3421 gtgcacacca tcctattgtt gtgactggga ggctgccacc atgcttcgcg ctttccctct
     3481 ggcgaaaaag ggagaaggac gggcccgtct gccttcactt tatgccagct tcggtaagct
     3541 gggtgagaca ccgactcata aagatatcat tgataacaat cgatccatca actggcctgt
     3601 ataacgtggc tgccggtgat taagaaagct gcacctacct aagtagtagc aaacgcacac
     3661 tttttagaaa aatcgatggt cagaaactgg attagcaatt ccgttccagg gttgcttttg
     3721 atttacgttg gcgtctgatc attgatttat cctcaaaagc ccaacctcat tggtaatgaa
     3781 ccagctccgt gaatgtccgc tctggcacag agcgaaattt tttgatctcc ccccctgaaa
     3841 tctaaactta gtcatgtcac gtttttgggt ttctaaaatt ttaacttcgc gtttttcgtt
     3901 gccgtaaggg ttatacagaa atgtccgtta agcagagttc aaaattgatt gccgtgatca
     3961 cgactggttt gaaagccgcg cccaagcctg tacagctctg gtttgcgttg attatgaacc
     4021 tgtcagccta aagcaagcgg atggacgatg agtattggta atctttcaga gtccggaaaa
     4081 gttcagcccc agtctgaaca ggcttgctgg cgccagtcca gtttcattca gtcgtggttt
     4141 ggttcttacg gcctgtgcaa tctacctcat taggcacatc ggcctgccag ataccggctc
     4201 ggggtgtatt tccgcttcca cgctgaatac tgttctcagc aatcctgggg tcatcacctc
     4261 ttctggtgtg ccttgcgcca taacatgtcc gtttgccatt accaccagtt gatcgcagta
     4321 ccggctagcc tgattaaggt cgtgcagcac agcgaccacc gttttcccct gagtccggag
     4381 ttcgcccatc aaccgcatca ggtccacctg gtgattgata tcaagatagg tggttggctc
     4441 atcaagtaat acaacgggcg tattctgggc caggaccatc gccagaaatg cgcgctggcg
     4501 ctgaccgccg gaaagctcgg ttaaccgacg aacggcaaga tgattgatcc gggtctggtt
     4561 catggcgaca ttaactcgtg cattgtcttc agcggagaga cgcccccaga gtgacagcca
     4621 gggattacga ccatacgaaa ccagctcctg gactgtgatc ccctctggcg ttaaatggtg
     4681 ctgaggcagc agcgaaagcc tgcgggccaa ctggcgcgat gagagcatat ttatgggatt
     4741 atcgccgaga aatacggtgc cagactgcgg cattaaaagc cgcgaaaaac agtttaacag
     4801 cgtcgatttc ccgcaaccgt taggaccgat cagggcggtg atcttccccg ttggcagtga
     4861 gagtgaaacg tcgttaagta ccttgtctgt cccgtaactg accgtcagat tttcagttcg
     4921 taaagtcatt tatcgcattc tcacaagcaa ccagacaaac cacggcgcac cgataatggc
     4981 ggtcagcacg ccaaccggga gctccagtgg gggatgaata attctcgcca gcagatcggc
     5041 aaccaccaac agcaacgcac ctgtcagggc tgaaacaggc agcagtctgc ggtgacgtcc
     5101 accggtgatg ctacgcatca tatgcggcac cacgagacca ataaagctaa tcgggccgca
     5161 ggcggccacg ccggtagatg tcatggcgac agctagtaac aaagcccaga atcgggtatg
     5221 gggcaccgac acaccgagcg tggtggcgcg cgcatcgccg agtgcaagga ggtcgagatc
     5281 gcggcaaaaa ctcaggctca gcggcagaaa taaaatcatc agcgggatgg caatcttcac
     5341 aaagctccag tcacggcccc ataagctgcc ggtcagccac agcagggcgt tgttcacatc
     5401 ctgtgggcgc gagagcatca gataatccgt caggctggcc cagcatgcag aaagcgccac
     5461 gccggtgagc gccagcttca tcggctggtg ggtctttgcc agcatcttca gtaatatcaa
     5521 ccccgccatg ccgcccgcaa aggccagcag cggcagcacc atcacgggca gtgacggcat
     5581 aagaagtaga gcccccacag aggccaggct ggcggcatgg ttaacgccga gaatatccgg
     5641 tgatgccaga gggttgcgca caatcccctg tatcagcacg cccgccacgg cgagggctgc
     5701 accgacaaac agtgccagca gcaagcgcgg cagtcggtac tccatcaata cataataatg
     5761 ctcgcgtccg gcctgccagt cggtcagcag cgcgcgccac ggcacgggga tcactcccat
     5821 atggagtgat aacagcgcac agcccgccag ggcaagggtg atgaaaataa ccagcgcaat
     5881 tttcatcctc gcctcctcac aagccagaca aagcaagggc tgccaatcag cgccagcact
     5941 gcgcctgcgg gcagatctcc ggggaaggcc agcgcgcgtg cgagtacatc tgccagcagc
     6001 atcagcgtgg cccccagcag catgctcact ggcagtacgt tgcgctgatc gaagcctgcc
     6061 cagaagcgcg ccagatgtgg caccagcaga ccgataaacg ccaccggacc tgccacgctg
     6121 acgcacgcac caaccagaag cagcactaac atattgatga ccaaacgtag cctcgtcagg
     6181 ttcactccca gcgtatgggc ggtgctgtcg ctgaggttga gcaggttcag ttgattcgcc
     6241 agcagcaaca cgacagggac tgcagtgacc accaccggca agagctgcca gacatcctgc
     6301 cagcgggcgt gggacactcc gcctgccagc cagtaaaaga tgccgtaagc atgatcttcg
     6361 gccagcagca gggtgatgcg ggtaaggccc atacaaaagg ccgacagcgc gatactcgcg
     6421 aggatcagtt tgtttctgtc atgggtatga cgaaatccgc ctcctgcggt catgaccagc
     6481 agccagctca cgccgccccc gcatgccgcg atgaacgaca gagaatagcc tgcaatcggc
     6541 gtcggactca gcgcgctggt aagcgccata gccagcgccg cgccgctgtt aatgccgagc
     6601 agtgaaggag aggccattgg gttgtgggtc agggtttgca gcagcgtgcc cgcgagcgcc
     6661 aggcttgcgc cgatcagaac ggcgaccagg cttcgtggca aacgaaggtt ttgcaccagc
     6721 gcttctggta gcgttggcgt gtgtccaggc agcagggcgc gggttgcatc tgctccggaa
     6781 acaggaatgg ccgagtagca aaacagactc agccagaaaa taataataag tgctgcaacg
     6841 ggaagccccc acagcagcac cgggtgtttt atcgcggtca tttcacaacg gtaagcggct
     6901 gatggtggaa gatttttacc gtgtcagcgg caatacgctc tgcagcaaaa ataccgcgca
     6961 tccgcgccca ggtgttactg tcgaccgaag caacctgctg cttctgcgcg gcggttaaca
     7021 tctgccagag cggatcttgt tgccagcgtt taacaatgct ctcttcgcga tagtgggcaa
     7081 ccagcagcca ggcaggattg accgccagca gttgctccag gccgatggac ggcatggacg
     7141 cacccgccat cgcagcggga acgttcagcc ccagagaggc cagcacgctg ccggtccagg
     7201 tctcctgagt atgcaggttg aattgctgtt cgcgtgatgt gccaaaggcc acgcgtgtcc
     7261 ctttgggaag ctggctggcc cactgcgcca tcctctcttt atgttgttcc aggcgtgcct
     7321 gcatctctcg ctttttaccc accatttcgc cgatgatagc cgcagattgc aaattttcag
     7381 cgtaggtttc gttgcgggac ttaagcagca gtaccggcgc gatttgctgc aaggcgatgt
     7441 aaacccccgc atggcgactg ctgtcggcaa tgatcaggtc tggtttcaga gcggcaatgg
     7501 cttccaggct cggctgcgcg cgcgttccga cggactgcca cggtttcagg tgcgcacgca
     7561 cttcgggcag gatgcgtttt gcatcgttat cgtcggcaat accgatcggg ctgacgtcca
     7621 cggcggccag cgcatcggcg aacgagagtt ccagcaccac aatccgttgt ggcgtttttt
     7681 cgagtgtaaa cgtgccgtgt tcgtcctgaa ccgtggcggc aaaggcgtgg ctgatcacca
     7741 gcagcaggcc tgcaaaaaca aaacggataa atgccaacat aatcacattc cagctaaaag
     7801 cccggcaagc cgggcgttaa cacatcagaa cttcaacgac ccctgcatat acagcgtgcg
     7861 cggctggcct gcatagatgc ctttgttgtt gtcgtcataa gagcggatga agtagtcctg
     7921 gtcgaagatg tttttcacac cgaatgccag gttcagatct gccatctgcg ggccaaaatc
     7981 atacgccacg cgtgcgcccc agagcatgaa gccgggaatg cggccggtac tgccgtcggc
     8041 gctctctttc accgtattgg cgttatccgc aaactggctg gactggaaat cgctgttcag
     8101 attgaacgtc cagtttcctg gcttgtagtc cacgcccagc gtgcctttat gtttcgggga
     8161 gaatggtacc agattgccgt aggtgtcgcc tttctcgcgg atttccgcgt tcacatacgc
     8221 atagctggcg tagatggaaa cgttatcaag cgttggcgtt agcgtaccca gatcgtaacg
     8281 tgcctgcgtt tccagcccgg tatggcgcgt tttgccacgt gcagtgacgg tgtcgttggt
     8341 ctggttggag tcgtactgat tgttaaagtt aatcaggaac agccccattt ccgccgtcag
     8401 cgcgccgtcg tcgtagcggg taccgagttc ccaggttcgc gctttttccg gttccacatt
     8461 gccgctttgc acagccttgc caatctggct gtactgtacg gtgccgaacg agccttcagt
     8521 gtttgcataa agattccagc tgtcagtcag gtgatagagc acgttcaacg ccggaagcgg
     8581 tgcgttatag ctcacttctt cgtgcgtgcc tgtgatggcg ttgttctggt atgactcgat
     8641 atgttcgaaa cgcatacccg gcgtgatggt ccagttgccg atgtcgattt tgtcatccag
     8701 ataccaggcg tgcgcctcgg tgccggaacg cgtatcgcgg tcgtaagggc ttgagccgga
     8761 cggcaactgc ccgctgctgg tggcggtgta gtaacgcatt tcatgcgttg attcattcaa
     8821 atagcgatag cccacgccca cttcgtgcgc ggaagggccg atcataaaga tctggctgta
     8881 gcgtggctca ataccgcgca cccagtagtt acgcggcgag agggtgatgc gtttgccttg
     8941 ctccaggtag ccgctgcgca gggtttgggt gtagaacccc tgaatgttga atttatgctg
     9001 gctgtctggc tggaactggt agcccaggct cgccagcttg cgacgacccc agaagcggtc
     9061 atacgggcgg gtggattgcc agcgatcggc gtcgtaatcc gcgcgagaca ggccaccggg
     9121 catgtcggct tcaccatcgt aatattgcag caggctgttg aaggtgtgca cctcatccgg
     9181 cgcatatttg cttttcagca tcaggtcgtc gatgcgggtg gcgctgtgct cgcgccagtc
     9241 actgccgcgc gtgccggagt agagcagcgc ggtgccaaaa ccgttgtccg ctgtgccgcc
     9301 caccatcagg ttgtgcgtct ctttcgggtt gttttgtgaa gaggttgggc tgagctgacc
     9361 ttccacgccc gcctcgatac caaagtcctg cggaatggca cgggtaacaa agttcaccac
     9421 gccgcccacg ctctgcggtc cgtaacgcac cgcaccacca ccgcgtacca cgtcaatggc
     9481 atccatgttg ccgagcgaaa cgggagccag tgaaagctgc ggctgaccgt aaggggcgaa
     9541 ggggacgggg atgccgtcca tcaggacggt cgagcggctg gcgaggcgcg ggctcaggcc
     9601 ccggatgcca aagttcatcg ccaggtcgtg gctgccggtg ccgttgtttt ccggcgcgct
     9661 gacgccaggg atgcggttaa gtacctcacg catggtggtt gcgccggttt tggcgaaatc
     9721 ctcacggcgg atcacgtcac gcgcgccagc atgttcaaat acgtcgtttt cacgcgcatc
     9781 acccagccag tcgccgacca cggtcagggc atcttctttt ggtgcgggcg cgggctccag
     9841 cgtccagctg ttatttccca gcggttttac ctgcagtccg ctgccgtcca gcagttgttg
     9901 caggccgctc tcgacgtcgt aatcgccgtg caggccgttg ctctgcttgc cgcgcgtcag
     9961 gctggcgtca accgagaggg taaatccgct gtgtgcggca tactgattga gcgctttatc
    10021 gagcgatccc ggtgcgatat taacctgtgc agcaaaagcg gaaaacgaga gaccggccag
    10081 cggcagcagg ctcaggcgaa tggcgttaac caaaggtgtt gttttacgaa aaacgcgtaa
    10141 cggcgtcata ccttccccat catcattttt gttgtgttca gctatgagtc gaacgagaag
    10201 ggtaaaaagg acaatcgaaa taagaattat tttccttaca gtggtgaaat gtttatccag
    10261 taccgcgtaa tagactgaat tttaacggga agcgtttgcg cgataacgtt caggatcgca
    10321 tcggtatttt tcagcgggaa cgtcccgctc aggcgcagcc cggcaacggc gggatcgcag
    10381 cgcagcacgc cgttgcggta acgggttagc gtggctatca cctcacccag cggtttatcg
    10441 ctgaagctca ggatgtcctt cgtccagctt gtactctcgt catccagcgg tttcactgcg
    10501 ccaaactcag aggcgctgaa ctgcaggctt tcaccagcgt tcacgatgcg tttttgcgcg
    10561 ggggcactgg cgagaagcac ttccacagcg tgctgctgca cgtcaagctg cgtgaaatta
    10621 tcctgctggc ggacggtaaa ttctgtccct aaagcagtga gctggccctg acgggtcagg
    10681 acacggaagg ggcgttgcag ggcatctttc gcggtagtaa tggcgatttc accgtaccag
    10741 agccggacgg tgcgctgatg cgcatcaaaa cgcacatccg ccgcgctttg ggtattcagc
    10801 gtgagcaggg agccatcttc cagttgctga cggctgactg tgcctttggc ggtgcggtaa
    10861 tctgcccgca gaccttcgcc ggtttccgac tgccagagct gccagcctcc gccagcgccg
    10921 agcaacagca gtaatccttt catcacgtga cggcgggtga ggcgggtatc gtgcaacgcc
    10981 ctgctggcta cgtcgccagg cacaccgcca agctggttgc gcaggttttc aacctgctgc
    11041 caggcccact ggttatcctg atcctgttca taccactgtt gccagcgcgc ttcctgttgt
    11101 gggctgacgc gctcgccgct tagcacggca taccagtggg aagctgaacg cagcgcctga
    11161 cggcgggaat cggttaacaa aggattcata acccatactc cagacggaac agcaggcagt
    11221 gctcgacggc tttcgccacg tattttttca cggagctgat ggaaacaccg agtttgtgcg
    11281 caatctcgct gtatgtcaga ccatccagtt gcgaaagcag aaacgcttca cgtgttttgc
    11341 cgtttagccc gtccagcatg ctgtcgagga gttgtagggt ctcgagttgg ctttcgcgtt
    11401 cctcaggtga aggcgctccc ccctccggca taagcgccag catctccaga tacgcttttt
    11461 ccagcgcgtt tcggcgaaac aggtccacca tcacgcgttt ggcgatagtg cagaggaagg
    11521 agcgaggatc gcggatcgtc gagagcgttt cgctgaccat tacccgcaaa aaagtgtcct
    11581 gggcaatgtc atctgcatca aaagcagact ggagtttgcg cgtcagccag cttttcaacc
    11641 agccgtgatg tgtgccataa agcgactcga acgttaagga agctgtggta gtggcgcggt
    11701 cagacatgcg gagtgcatca aaagttaatt atcacgtagt catattaata tgagaatggt
    11761 tatcattaca attggaaata aaattgtttc caatagacat ttttaacatg ttgtttttct
    11821 aagtgttata aggtaggtat aaaatgggat ggagcctctg cttctggcat gtgtcggtca
    11881 gaatgactca tgatgtggtc tgctattatt gacatcctca ctgccctaaa ggatggggat
    11941 ttcggtaatg ctgccaactt actgatttag tgtatgatgg tgattttaag gtgcttgcgt
    12001 ggcttccatt tccatcagat gtccttcctg ctccgctact gaaggcgtgg tgcgtaacgg
    12061 caaaagcact gccggacatc agcgctatct ctgctctcct tgccgtaaaa catggcaact
    12121 acagttcact tacaccgcct ctcagcccgg taagcaccag aaaatcattg atatggccat
    12181 gaatggcgtc ggatgtcgcg ccagtgcacg cattatgggc gttggcctca acacggtttt
    12241 acgtcactta aaaaaatcag gccgcagtcg gtaacctcgc gcatacaacc gggcagtgat
    12301 gtgattgtct gcgctgaaat ggacgaacag tggggctacg tcggtgctaa atcacgtcag
    12361 cgctggctgt tttacgcgta tgacaggata cggaggacgg ttgtggcgca cgtcttcggt
    12421 gaacgcactc tggccacact ggagcgtctt ctgagcctgc tgtcggcctt tgaggtcgtg
    12481 gtatagatga cggatggctg gccgctgtat gaatcacgcc tgaagggaaa gctgcacgtt
    12541 atcagcaagc gttacactca gcgcattgag cgacataatc tgaatctgag acaacatctg
    12601 gcaaggctgg gacggaagtt actgtcgttc tcaaaatcgg tggagttgca tgacaaggtc
    12661 atcgggcatt atctgaacat aaaacactat cagtaagttg gagtcattac ctcctcccga
    12721 actcaaagct atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt
    12781 gtgatcttac ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg
    12841 attcacaaga caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt
    12901 cgtagttaag atctgaagct tgcagaatgc atcggtgtta ctgccggagt tcgtgaactt
    12961 agtctgcatg aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact
    13021 tacttaatta tatcgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc
    13081 tcagtccgga acaatttgaa aatcagaatc tcacttaaaa cggcatccat tttacaaaag
    13141 atggccgtct gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga
    13201 caaggcgata tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta
    13261 gtaaaaaaga gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca
    13321 caaaaaaaaa ccattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt
    13381 tatatagcaa aatatgcttg ttacacctgt cataattagg catggtaaaa ttattgactg
    13441 aaccctgggg gagaaagatg cacagtgatt attataatat ggtttttggt gaaaaattgg
    13501 ccaatatttt atatgaagct aactctcaat ttttttacga gagaaatgtc atagaagagg
    13561 cagtcaacgc tttattctgt gagcgtgaaa taataaacaa caagaacata ataaagaagc
    13621 tgatgttctt tttgtcagat gttaatcaca ccaagaaaga tgttgttcag agcgcactta
    13681 atattatcat tgacattaca tcaggtgata tttgagattt tacataaaag ataatctctc
    13741 aaggagtctt atctgaaaac gaaatatata gtccctctcg ttttgtaata tcgattggct
    13801 ttgttgaatc ggttacgtac cccggagccg gtcatatttt caggtaatac acacgctttc
    13861 agacatttcg gctttcgtca ttttggtatc atgggcatag cttctgctac ctgaccatca
    13921 taatcacgca gcttcagtga agcagtgacc acctgtttaa cccggtgtat cgttatgatc
    13981 ttatccagca attgaaccgc cccgggtttc ctggagagtg ttttatctgt gaactcaggc
    14041 tgccagatca tcgtttccga tggaagcata ataagctttt tctgcttctg ccggtggaat
    14101 atggccctgc ctttccagca atcgtcgatt gttataccag tccacccacg tgagtgtggt
    14161 cagctcatca tactgacacg tgcattaccc ctaccgcgat aatgcaaccg tacaatatag
    14221 ggcgtttcct aaacgcagcc ctcatcactc caatccaaca agacacagca tttttgatca
    14281 cgaagaaaga caaataattt ccgtaaatgt ttccgttgtt acaaataaca acagtgtttt
    14341 atatttcttt ggcctcaggc ccgcgcatgt tgccattcga tgtcattttc ctgactcgtt
    14401 tcttctttat ggtgtagtgt gatcttcact ttatggatgc taacaatatg aaaagaaaaa
    14461 ttattcccgt gctaatcggt tgtacgctct ctttctctgc cctggcggca caacctacgg
    14521 ctgagcgcta tgtcgtcagt tttccagaag gcacgcacgt gaactatgcc ggcgcatttg
    14581 ccagtgcatt cccgaacggc cttccggttg ggataggttc tggcctgctg ttcaccggta
    14641 aacagggcga tgccctgaca ttcgcaacca ttaccgatcg cggtccgaac gcggattcac
    14701 cgaaagaggg gaaaaacgaa actaaaattt tcgttacgcc agatttcgcg ccgctgctga
    14761 tgacgattcg tgtacaaaac ggcaaagcgg aggccatcga tccccgtcct ctgcatgacg
    14821 ataaaggcgc gatcaacggt ctgccgctgg caagcgacgt gattggctcc accaatgaag
    14881 tggccttcag cgacacgtta catagactga agggggataa tcgcgggctg gatacggaag
    14941 ggatcactcc ggatggtaaa ggaggttact ggctgtgcga tgagtatggc ccgttcctga
    15001 ttaacatcga cagtaaaggg aaaatcctgg caatccacgg tccgcaggcg gcggaagggg
    15061 aaaaagcgat tgcgggcggt ttgccaaata tcctcaaatg gcgtcaggcg aaccgcggct
    15121 ttgaaggcct cacccgtatg ccggacgggc gtattattgt cgccgtgcaa agtacgctgg
    15181 atatcgacgc taagagcaag aaaaaggcgc tgtttacgcg tctggtgagc ttcgatccgg
    15241 caagcggaaa aaccgcgatg tacggttatc ctatcgacag cgcggcctac agcaaaaaca
    15301 gcgacgctaa aatcggagac atcgtggcgc tcgataacca gcacatcctg ctgatcgaac
    15361 agggaagaga taaaaacaac agaatgcgta acctgattta cgaggtcgat ctcaataagg
    15421 cgagcgacct gtcaggcttc gacaagccgg gtgagtatcc ggagtttgat gatgagaaaa
    15481 ccctgtcaca gcgcggcatt acccttgcgc agaaaacgca ggttgttgat ctgcgctcct
    15541 tgggctggca gcaggagaag gctgaagggt tggcgctgat cgacagcaaa acgctggcgg
    15601 tggcgaacga taacgacttt ggcgtgaagg tggcgatgca acatccggtc gagggcaaga
    15661 cattcaagga ttatcgggtg aacgcggaag gaaagctgac gcttgacgac aagcaggttg
    15721 aaacgaccct cagagtgaag ccactggaga aaccggagtc tgacagtgaa ctgtggattg
    15781 tgacgctgcc ggaagcgttg aaataagttt ataaggtacg gacagcagta tcgcttagtt
    15841 tttcaaattc atgtatgtga acaatattgg ggttccggcg tagaccccca atgaaagcgt
    15901 caccacgtga aaacatgcct gcacggttta cacgtcatct catggtggca gccggttaac
    15961 tttcctgcta caccagtgga aaaaggccat aagcagtccg aacaaa
//
LOCUS       DAZFWL020000102        15993 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFWL020000102 DAZFWL020000000
VERSION     DAZFWL020000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.102"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            103..531
                     /gene="uspC"
                     /locus_tag="ACHKNP_RS17515"
                     /old_locus_tag="pgaptmp_003503"
     CDS             103..531
                     /gene="uspC"
                     /locus_tag="ACHKNP_RS17515"
                     /old_locus_tag="pgaptmp_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HGU4705606.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(538..1962)
                     /gene="otsA"
                     /locus_tag="ACHKNP_RS17520"
                     /old_locus_tag="pgaptmp_003504"
     CDS             complement(538..1962)
                     /gene="otsA"
                     /locus_tag="ACHKNP_RS17520"
                     /old_locus_tag="pgaptmp_003504"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HGU4705607.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1937..2737)
                     /gene="otsB"
                     /locus_tag="ACHKNP_RS17525"
                     /old_locus_tag="pgaptmp_003505"
     CDS             complement(1937..2737)
                     /gene="otsB"
                     /locus_tag="ACHKNP_RS17525"
                     /old_locus_tag="pgaptmp_003505"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HGU4705608.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2904..3890)
                     /gene="araH"
                     /locus_tag="ACHKNP_RS17530"
                     /old_locus_tag="pgaptmp_003506"
     CDS             complement(2904..3890)
                     /gene="araH"
                     /locus_tag="ACHKNP_RS17530"
                     /old_locus_tag="pgaptmp_003506"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HGU4705609.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3905..5419)
                     /gene="araG"
                     /locus_tag="ACHKNP_RS17535"
                     /old_locus_tag="pgaptmp_003507"
     CDS             complement(3905..5419)
                     /gene="araG"
                     /locus_tag="ACHKNP_RS17535"
                     /old_locus_tag="pgaptmp_003507"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HGU4705610.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5489..6478)
                     /gene="araF"
                     /locus_tag="ACHKNP_RS17540"
                     /old_locus_tag="pgaptmp_003508"
     CDS             complement(5489..6478)
                     /gene="araF"
                     /locus_tag="ACHKNP_RS17540"
                     /old_locus_tag="pgaptmp_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HGU4705611.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7275..7778
                     /gene="ftnB"
                     /locus_tag="ACHKNP_RS17545"
                     /old_locus_tag="pgaptmp_003509"
     CDS             7275..7778
                     /gene="ftnB"
                     /locus_tag="ACHKNP_RS17545"
                     /old_locus_tag="pgaptmp_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HGU4705612.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7857..8108)
                     /gene="yecJ"
                     /locus_tag="ACHKNP_RS17550"
                     /old_locus_tag="pgaptmp_003510"
     CDS             complement(7857..8108)
                     /gene="yecJ"
                     /locus_tag="ACHKNP_RS17550"
                     /old_locus_tag="pgaptmp_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HGU4705613.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMQAIQKAIALIELKFTPQGESH"
     gene            complement(8223..8309)
                     /gene="azuC"
                     /locus_tag="ACHKNP_RS17555"
                     /old_locus_tag="pgaptmp_003511"
     CDS             complement(8223..8309)
                     /gene="azuC"
                     /locus_tag="ACHKNP_RS17555"
                     /old_locus_tag="pgaptmp_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HGU4705614.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8572..8895
                     /gene="yecR"
                     /locus_tag="ACHKNP_RS17560"
                     /old_locus_tag="pgaptmp_003512"
     CDS             8572..8895
                     /gene="yecR"
                     /locus_tag="ACHKNP_RS17560"
                     /old_locus_tag="pgaptmp_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HGU4705615.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9066..9563
                     /gene="ftnA"
                     /locus_tag="ACHKNP_RS17565"
                     /old_locus_tag="pgaptmp_003513"
     CDS             9066..9563
                     /gene="ftnA"
                     /locus_tag="ACHKNP_RS17565"
                     /old_locus_tag="pgaptmp_003513"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HGU4705616.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(9601..9840)
                     /gene="yecH"
                     /locus_tag="ACHKNP_RS17570"
                     /old_locus_tag="pgaptmp_003514"
     CDS             complement(9601..9840)
                     /gene="yecH"
                     /locus_tag="ACHKNP_RS17570"
                     /old_locus_tag="pgaptmp_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HGU4705617.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10031..11242
                     /gene="tyrP"
                     /locus_tag="ACHKNP_RS17575"
                     /old_locus_tag="pgaptmp_003515"
     CDS             10031..11242
                     /gene="tyrP"
                     /locus_tag="ACHKNP_RS17575"
                     /old_locus_tag="pgaptmp_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HGU4705618.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11304..11969)
                     /gene="yecA"
                     /locus_tag="ACHKNP_RS17580"
                     /old_locus_tag="pgaptmp_003516"
     CDS             complement(11304..11969)
                     /gene="yecA"
                     /locus_tag="ACHKNP_RS17580"
                     /old_locus_tag="pgaptmp_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HGU4705619.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12165..12251)
                     /locus_tag="ACHKNP_RS17585"
                     /old_locus_tag="pgaptmp_003517"
     tRNA            complement(12165..12251)
                     /locus_tag="ACHKNP_RS17585"
                     /old_locus_tag="pgaptmp_003517"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12215..12217),aa:Leu,seq:taa)
     gene            complement(12264..12337)
                     /locus_tag="ACHKNP_RS17590"
                     /old_locus_tag="pgaptmp_003518"
     tRNA            complement(12264..12337)
                     /locus_tag="ACHKNP_RS17590"
                     /old_locus_tag="pgaptmp_003518"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12303..12305),aa:Cys,seq:gca)
     gene            complement(12392..12467)
                     /locus_tag="ACHKNP_RS17595"
                     /old_locus_tag="pgaptmp_003519"
     tRNA            complement(12392..12467)
                     /locus_tag="ACHKNP_RS17595"
                     /old_locus_tag="pgaptmp_003519"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12432..12434),aa:Gly,seq:gcc)
     gene            complement(12619..13167)
                     /gene="pgsA"
                     /locus_tag="ACHKNP_RS17600"
                     /old_locus_tag="pgaptmp_003520"
     CDS             complement(12619..13167)
                     /gene="pgsA"
                     /locus_tag="ACHKNP_RS17600"
                     /old_locus_tag="pgaptmp_003520"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGU4705620.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13224..15056)
                     /gene="uvrC"
                     /locus_tag="ACHKNP_RS17605"
                     /old_locus_tag="pgaptmp_003521"
     CDS             complement(13224..15056)
                     /gene="uvrC"
                     /locus_tag="ACHKNP_RS17605"
                     /old_locus_tag="pgaptmp_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGU4705621.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15053..15709)
                     /gene="uvrY"
                     /locus_tag="ACHKNP_RS17610"
                     /old_locus_tag="pgaptmp_003522"
     CDS             complement(15053..15709)
                     /gene="uvrY"
                     /locus_tag="ACHKNP_RS17610"
                     /old_locus_tag="pgaptmp_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HGU4705622.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            15851..15967
                     /locus_tag="ACHKNP_RS17615"
                     /old_locus_tag="pgaptmp_003523"
     CDS             15851..15967
                     /locus_tag="ACHKNP_RS17615"
                     /old_locus_tag="pgaptmp_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4705623.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
BASE COUNT         4017 a         4083 c         3715 g         4178 t
ORIGIN      
        1 cagactaata tttacctgtt tgaccgagtt gggattgcgt cgtttctcca ttaggagtaa
       61 agctttaatg tcacctgaag ttcacagaat aaggaacagg ttatgagcta tagcaatatt
      121 cttgtcgctg ttgcggttac accggaaagt cagcaactgc tggcaaaagc ggtatctatc
      181 gccaggccag taaagggaca catcagttta attactctcg cttccgaccc ggaaatgtac
      241 aatcaattag ccgcgccgat gcttgaagat ttacgcagcg tgatgcaaga agaaacgcaa
      301 agctttcttg ataagttaat tcaggatgcg gggtatcccg ttgacaagac gtttattgcc
      361 tacggtgagt taagcgaaca tattctggaa gtatgtcaca aacatcattt cgatttggtg
      421 atttgcggta atcacaatca cagtttcttt tcgcgagcat cctgctcggc gaaaagagtt
      481 attgcctcaa gtgaggtcga tgtgctgtta gttccactta cgggagatta accgctccta
      541 cgcaagcttt ggaaaggtag caactttatc gcgctgctgg ctttccgcgc ttcgcggaac
      601 tatctgcttt aggtcgctaa tgaagcactc ctgccagtgg ttaatatcgt ttttcacgat
      661 aacgtccagc atttctgcat gacgggaaat acgttccgcc agcgacatag tcaatgcacg
      721 atccagcgca gctgcaactt cgtcacgatc gtaggggtta acaattaacg ccgacgttaa
      781 ctcgtttgcc gctcccgcaa attgcgaaag aacaagaacg cccgggttgg ctgggtcctg
      841 agcagcaaca tactcttttg ctaccaggtt catcccgtca cgcagtggcg tcactaagcc
      901 cacgtcagag tagcggaata ttttcatcag taatttacgg tcaaaatgct gattcaaata
      961 ataaagcggc gtccagccta attgcccgta tttaccatta attcgtccag cttcattttc
     1021 gagctgatga cgaatatcct gataggcttg cacatcacca cgcgacgttg gtgcaatctg
     1081 ggtataacga attttaccat gatgctgcgg atatttttcc agcaacgctt cataggcgag
     1141 aaaacgctct ggcaaacctt tggaataatc cagccgttcg acagaaaaga tattttgtac
     1201 gtttttcagt tccgctttaa gttgcgccag ttttggcggc agtggcccgg cagcctgttt
     1261 ggctatttct ttcggttcaa tgccgatcgg gtagacttct gttcgaaatg ctttgcccca
     1321 ggctgtatgg ctttttgcgc tacgtgtcgt gacgcgggtc aggttagaaa gacaatccag
     1381 gaacgccaga cgatcgtttt ctgtctggaa acccagcaaa tcataatcac aaagctgttc
     1441 aagcaaggtg tcatatgtcg gcagcgcgtt gaagatttcc ggtgtcggga aaggaatatg
     1501 cagaaagaaa ccaatgcgat tattcactcc ccgtttgcgt aattcatgcg caaatggcaa
     1561 caggtgataa tcgtggatcc agataatgtc atcgtcttgc aacagcggca gtaatttatc
     1621 tgccagcaac gcatttacgc gtagatagcc gtcccaggca ggacgctgaa attgcaccag
     1681 atcgagccga taatgaaaag cgggccagag aacggcattg gagaattggt tgtagtattc
     1741 gtcaaggtcc tgttcgctga ggttaaaaga ggcccacgta atgttacctt ttttcacctt
     1801 ttttagcggc tgatcctcat tccctgtttc accactccag ccaaaccaca gtccgcctgc
     1861 ggctttcagt gcccccagta tgccaacggc aaggccaccg gcactggcgg cgtgctcgtc
     1921 tggtggtgca atccggttag atactacgac taaacgactc atagtcatca ctcctgttat
     1981 tttctctttt ttgttgtaat gcggtggtta tcatttcaag ccagctccag acatccggca
     2041 cacccgccag tcgccatgat gcctgagttg cacctgtgcc aatttttact gacattccgc
     2101 ccagtcggtt aacgactgcg aagccagatt catcggttaa atcatcgccc agaaatacgg
     2161 gcgttcgccc gataaaggga gcttcctgca taaaagctgc aattgcctca cctttactgg
     2221 tacctctcgg tttgatctcg acaacacact ttccctgctg taacgccatt tgtggccaga
     2281 tctgagtaat acgttgcgct aatgtcatta atgcgtcttc atgctgcgga gcctgacgat
     2341 aatgcagcgc aaaagccatc cctttcgcct ccagctccgc gccgggatac tgagcgatga
     2401 ctgtatgcag ttgcacgcta atatcacgcg caatcgcatc cggcagatga acgatatgtg
     2461 ttttaccatt gatgtcacgg cgctccgccc catgcacgcc cgctaacggg aagcgataag
     2521 gttttgccag tgcgtcaagc tccaccattg agcgccctga tatcaatgcc aatgcaccat
     2581 cacttgcggt tgccagtagc tgtagtcctt gcagaatatt gtcaggcacg acgacctgat
     2641 cgggatgcgg tttgatttcc gccagcgttc catcaagatc aaaaaaccag gcatatttcg
     2701 cggatagttc aggggtttcg gttaacggtt ctgtcacccg gctctcctcc ttctcttttc
     2761 ttattgtttt gcgcagaacg cgctttacag acatgtaagt atagacagtg tgacgggggt
     2821 cgccatttgc aatgaagaac agccaaccgt tgtggcggct ggctgtggtg ggaaaaaacg
     2881 ttaaattgtt gtggaaaaaa gcatcagaca gtgcgtttcg ctttttgctt gtaacggtcg
     2941 aagatcaccg ctgccagcag gattaagccg cgaaccacgt actgcgcgaa aggagaaata
     3001 ttaagcaggt tcatggcgtt ttccacggtg cctaaaatta agatacccgc caccacatat
     3061 gagatttttc cgatgccacc tttcagagaa acgccaccta aaacgcaggc ggagataaca
     3121 ataagctcat aaccaatcga cgtcattggc tgcccactag tcatacgtga agccagaata
     3181 attccggcta tcgcagatac caggccagag agaacaaaga taataatttt ggtgcgaaca
     3241 accggtacac ccgccagacg cgcagcctct tcgttcccgc caatcgccag ggtgttacga
     3301 ccaaaggtgg ttttattcag caacaaacca aagataatca gacacgcgac ggtgagccag
     3361 attggcgcag gcagaccgaa ccagttagcg taaccaaggg caaagaagct ttcatcttcg
     3421 ataccgaccg ctttaccgtc tgaaatgatg tacgccagac ctcgaacaat ctgcatcgtt
     3481 gccagtgttg tgatcagagc atttattttc agtttggcga taacaaagcc attgaccagg
     3541 ccacagagaa tgccaagcag caaccccgct gccacgccaa tccacaggct ttcagtcagg
     3601 ttgataacca ccgccgtggt gacacccgca caggcaatta cggaggcgac agaaaggtca
     3661 aagtcaccgg aagcaaggca gaacaacatg ccacacgcca ccatccccga catggaaatt
     3721 gccaggccca accctttcat attaatgaag gtggcaaaat ttgggacaaa aatggcacag
     3781 gcgataaaga gcaccgcaaa caccaccagc atgccgtact gatcccagat acgcccgaag
     3841 ctgaatgacg acttaggtgc gccagacccc gatgtagaaa cagaagacat catactctcc
     3901 ttactcaggc aactgcctgg ctgactttag gcatcgcaag gctcagtgcc tgacgctcat
     3961 ctgcctgctc gtgtaacaat tcaccggcga tttcaccttc ccgcatcacc acaatccggt
     4021 cggcaacgcc gaggacttca ggtaagtcgc tggaggcaaa cagcaccgcc acaccctgcg
     4081 ccgccagcgc ataaatcacg ttgtaaattt catgcttagc accaacatca atgccgcgcg
     4141 taggttcatc cagcaaaatg accttcatct cttccgataa ccagcggccc agaatggctt
     4201 tttgctgatt tccgcctgag agattcatga tcagttgctc agcgcccggc gttttgatgt
     4261 tgagcgaacg aatgtggtga tcggcattgt tttcttccca accgttgttg attacacaac
     4321 cgccgagcac atgtttacgt ctggcactga tgttgatatt gtcgcgaacg gagtgcacgg
     4381 gaataatgcc ttccgctttg cgatcttccg ggcagagcat catgcctgcg gcaatggcgt
     4441 ggctcggttt acgaatatcg atcggctgtt ggtcgatata aacctgaccg gcggtgattt
     4501 gcgtcccgcc aaacaagcct ttcattaatt cgctacgccc cgcccctacc agaccaaaca
     4561 gcccaacaat ttcaccactg cgaaccgcca gacttattgg cgtacgcacg cctggtgctt
     4621 tcacagcatc aagacgcagg cgctcctcgc cataactacg aggttgccag ccgtagatat
     4681 cgccaatgtc gcgcccgacc atcgcctgca ccagcgcgtc gtggtcaacc tgctgcatat
     4741 cggtaaaggt tttgacataa cgtccatctt taaagacagt gatggcatcg ctgagggcaa
     4801 atatttcttc catacggtga gaaacgtata agatcacccg cccctctttt cgcagttcac
     4861 gaataacgcg gaaaagattg tcgatttcgc gggcagaaag ggagctggtt ggctcatcaa
     4921 aggcgataat tttagcgtta cgcgccagcg ccttggcgat ttcaaccatc tgccactgac
     4981 caatggagag atatttcagc ggcgtgtccg ggtcaatatc cataccaaga tgtttaagtt
     5041 gtaaacccgc ctcataattc agcaatgagc gattcacaat gccgccttta tgcggcagct
     5101 ggccgagata gatgttttcc gcgacggtca tttccggcac gagatgtagt tcctggtaaa
     5161 taatcgccac acccgcgtta agtgctgcgg tcgtgtcgga aaaggacatt tcctgcccat
     5221 taatcactac agaacccgtg gttggcgcat agttgccgct gaggattttt aagagagttg
     5281 attttcctgc gccattttca cccatcaacg catgaacctg accggcatag cagtcaaaac
     5341 taatatccgt cagcgcctta acgccgggaa acgttttacc gatgccgcga aatgagagat
     5401 acggggtaga ctgttgcata acgtctccgt gaatcactgg tcgttcacac cccctctgca
     5461 tcatgcagag gggaattttt ccggcaaatt acttaccgcc taaacctttt ttctccagtt
     5521 cttctttaaa gttgtcacgc gtgatcagta ccacgtcggt aacttcggta aattttggcg
     5581 gttcaacgtc ttttgctacc cagttgtaaa gcatttcgct ggatttatag ccatgtacgt
     5641 ccgggcttgg cagcagggaa ccgtagaagc cggttgcctg tgctttagac agttcgctca
     5701 ccgcatccac accgttaatg ccaatgccga tgatatcggc cgctttaaag ccctgacctt
     5761 ccgtcgcgcg tacgccgccc agcacggtgc tgtcgttcat accgacgatc agccaatgtt
     5821 taacttccgg atgttgaacc agcattgagt tggcagcgtc aaatgccccc gggatgtcgt
     5881 tagatttggt aggtacctga taaatttgtt tttccgggaa tccggccgct ttcagcgcat
     5941 ccatagatcc cgtagtacgg cggcgggcgg tatccagttc gttggcggta atcgccatca
     6001 ccgcgctttc tttgacatcc cagccacgtt tctgcatctc tttatacagt tcctggccct
     6061 gacgttcgcc aattttagtc gccgccatca tcaccagcgg aacggtatcc attggcttac
     6121 ctttggcgtt aacaaactgg tcatccacgg caatgacttt catatcgtag ccacgcgctt
     6181 tcgcgacgat ggcagagccg agtttggggt ccggagtaca aataacgaaa ccttttgcgc
     6241 cactggcagc caggctgtcg atcgcgttca atgttttttc gccatccggc acggcaatct
     6301 taataacctc aaaccctaaa tccttcccgg ctttatcggc aaacttccat tcggtctgga
     6361 accacggctc ttccggttgc ttcaccagaa aaccgagctt caggttctcc gccatagcgg
     6421 attgtgacat aacggctgcc agaccaatgg ctgccagggc tttagtaaat ttgtgcatgg
     6481 ttctctccag ctttagtgtc gttttgtgta gggcaaaaac gaatgacatt cgttaaatta
     6541 atcggaaaac aaagcattac cttttaacta aaagataagt gactgtgttg acatagtttt
     6601 agcgagaaat taattctcca taggagagca atatcacatc gcagaattac agtgagaacg
     6661 tgcataaatt tagcgggaaa agacataagg gaaagccaat ttgtcagaca aattgtcgaa
     6721 tgcacagcag attaatccat aagattagcc tggaaatcct tgttgtcttt ggtacccatg
     6781 cgggatgtct tctttttaac cagtcaatag gccgcattac ctggcgttga gtttttgaaa
     6841 tggtgtaata accgcaactc aaagatgtgg aaaatgcacg tcattcattt cgccattaat
     6901 tatcactgta ctcattaatt aacagaacac gtataatgag agccatctcg caaaaatgaa
     6961 aaaacgtttt ataaaatcat cacttcatca tgaattcaaa ttcattgatt aatatcaaca
     7021 agatacaaaa agcgctatca ttaaaattca ttgcagttac attgatttca tcaatgaaat
     7081 gtaaaaatat ataaacttga tgatttaagc attttcttat acccgttcag acgttattct
     7141 tatttcagat catcgtcaga attgactcca cgatcacatt tcggaccggc agaaaggaat
     7201 tattctgcaa acagtaatta tggtgttttg atttatcttg cacctctcca cttctggata
     7261 taaggatatt aggtatggca accgctggaa tgcttctcaa actcaactct caaatgaacc
     7321 gcgagtttta cgcatccaat ctctaccttc acctgagtaa ctggtgttct gaacagagtc
     7381 tgaacggcac cgccactttc cttcgcgccc aggcacagag taatgtgacc caaatgatgc
     7441 gcatgtttaa ctttatgaag agtgtcggcg ctacccccat cgttaaagcc attgatgttc
     7501 ccggtgaaaa actgaactct ctggaagaac tgttccaaaa aacgatggaa gaatacgagc
     7561 aacgttctag tacgttggca cagttagccg atgaagcgaa agaactgaat gatgattcaa
     7621 ccgtcaattt cctgcgcgat ctggaaaaag aacagcagca tgatggtctg ttgctgcaaa
     7681 ccattcttga tgaagtgcgc agtgcgaaac ttgcgggtat gtgccctgtg cagaccgacc
     7741 aacatgttct gaatgtcgtg tcacaccagc tgcattgatc atcatcggcg ctaatgcatt
     7801 gcgccgatga aggttttgag aaaccgcagc ctcatctgtt tgaagcagcg gtttttttaa
     7861 tgggattcac cctgtggggt aaacttgagt tcaataagcg cgatggcttt ttggattgcc
     7921 tgcatggtga ccgggtctgc ggcggcggga tggttagtaa agtcgatatt cttcagctga
     7981 ctggacattt tttcacgaac ttcaacgggc gcgattacat cgagaacatc cagaatttgt
     8041 ttgataacca actggcaagc aaccacatca gaaaccagtt cctgatcggc attcagcggc
     8101 tgggacatcg taaactcctg atagcatttt gaaagccgtt atagtagcgg cttcacatct
     8161 tcagcgatag tcacatccac cgtcatcagg acacaaaaaa acctgccgga gcaggttttt
     8221 tattatcgga acatattgcc tggcggtacg tctttgaacg tcttgcaata gttattgaac
     8281 atacttttca ggattttgcg cagtttcatc gcggcactcc gaccatttgt tatacaggtg
     8341 ttattctctt tgcgcttata atatgacaac catcacaaaa atcaatcttt atgtgataca
     8401 aatcacataa ataccccttt aatgttataa aaatgataat caaaaaacag cccccctatt
     8461 tctgacacct acagatggca agaaatagcg cctgccaggc gtcttttccg gccattgtcg
     8521 cagcgctgta acgcgtaaaa tagtgctttc tcttactctt ctggctggac catgagactt
     8581 ctgattctga ctctttcatt aataacgctg gcggggtgta cggttactcg ccaggcccat
     8641 gtgagcgaag ttgacgcggc aacaggtatt gtacggttgg tttatgacca ggcttttttg
     8701 cagcatgccc ataccgatcg ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag
     8761 gaaggctata cccacgcggt cccctttggt cagccagtag gcaactgtag cctttttgcg
     8821 ggttccttat gtttgaatac ggaattcact ttgtcatatc agtgccatca ttccgccttc
     8881 cctgtctttc tctaaatatt ttaatcagcg aggggatctt cgctgattaa agaaatagcg
     8941 gtaatgcgtt ttattcccat tcgtattttt aataattgaa gtttatattt taccttttgc
     9001 aaataataaa ataacaaatt atagtgacgc cacagcaaca aatataacct ttgtggagca
     9061 ctatcatgct gaaaccagaa atgattgaaa aacttaatga gcagatgaac ctggaactgt
     9121 actcttcact gctttatcag caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg
     9181 ctgccgcgtt cctgcgccgt cacgcccagg aagagatgac gcatatgcag cgtctgtttg
     9241 attacctgac tgataccggc aatttaccgc gtattaatac cgttgaatct ccgtttgctg
     9301 aatattcctc acttgatgaa ttattccagg aaacctataa acacgaacaa ttaatcacgc
     9361 agaaaattaa cgaactggct catgctgcaa tgaccaatca ggactaccca acatttaatt
     9421 tcctgcaatg gtatgtttct gagcagcatg aagaagagaa actgttcaaa tcgattattg
     9481 ataaattaag cctggcaggc aaaagcggcg aaggtctgta ttttatcgac aaagaactct
     9541 ctaccctcga cacacaaaac taatgctctc aggcggcagg ttttctgccg ccgtttcaaa
     9601 tcagtgacgg caaatcttac tctgatcggt cgaaaaacct tcttctgaag gtataaattt
     9661 gccttttgct gccagaaacg ctaccagctc tcccgcagtc atcccttctg ccgagcaggt
     9721 gtgaaaacgt gcctgttcac caaaacgcgc tttaattgct gcttccagac tggcatgcgt
     9781 atattgctcg cctgattcaa tcatcatatt taacacttca tgaccgtgaa tagagtccat
     9841 cgtccctcct caaaaaaagc ctagcgtagc gattgccgct tatgaagact ttgcgccagc
     9901 gcaggactga atgcttttta ttgtacattt atatttacac catatgtaac gtcggtttga
     9961 cgaagcagcc gttatgcctt aacctgcgcc gcagatatca ctcataaaga tcgtcaggac
    10021 agaagaaagc gtgaaaaaca gaaccctggg aagtgttttt atcgtggcgg gaaccacaat
    10081 tggcgcaggc atgctggcaa tgccgctggc tgcggccggt gttggtttta gcgttacgtt
    10141 aatcttgttg attgggcttt gggcgttgat gtgctacacg gcgctattac tgctggaggt
    10201 gtaccagcat gttccggcag ataccggtct tggcacgctg gcaaaacgct atctgggacg
    10261 ctacggtcaa tggctgacgg gcttcagtat gatgttctta atgtatgctc tgactgcggc
    10321 atacatcagc ggtgccggtg aattgttggc ctccagcatc agcgactgga caggtatttc
    10381 aatgtcggca accgctggcg tgctgttgtt cacttttgtt gccggtggcg tggtttgtgt
    10441 tggaacatcg ctggtcgatt tatttaaccg ttttctgttc agcgccaaaa ttatttttct
    10501 agtggtgatg ctggtattgc tgctaccgca tattcacaaa gtgaatcttt taaccctgcc
    10561 gttgcaacag gggctggctc tgtcagcaat cccggtgatt tttacctcat ttggttttca
    10621 cggtagcgtg ccgagtattg tcagctatat ggatggcaac gttcgtaagc tacgctgggt
    10681 gtttataacc ggtagtgcga tccccctggt ggcatatatt ttctggcagg tggcaacgct
    10741 tggcagcatt gattcaacaa cctttatggg attgctggct aatcatgctg gattaaacgg
    10801 gctgttacag gcgttacgcg aaatggtggc ctctccgcat gttgagctgg cagtgcattt
    10861 atttgctgat ttagccctcg ccacgtcatt tctcggcgtt gcgttaggct tatttgatta
    10921 tctggctgat ttatttcagc gttcaaatac cgttggtgga cggttgcaaa ctggtgcaat
    10981 tacctttctg ccgccgttgg cgtttgcact gttttatcca cgaggatttg tgatggcgct
    11041 gggttacgcc ggtgtggcgc tggcggtact ggcattgatt atcccttcgc tgttgacctg
    11101 gcaaagcaga aagcacaatc ctcaggcggg ttaccgggtg aaaggtggtc gtccggcgct
    11161 ggtggtggtg tttctctgtg gtattgctgt gattggcgtg caatttttga ttgcggcagg
    11221 gttgttacca gaagtggggt gatcagatag cctcaaattc cttattgggt gccagaatta
    11281 acgctgacac ccaatttgac ctcttaatgc aggcagcact gcttaaattt cttaccacta
    11341 ccgcacgggc aaggatcgtt acgccccggt ttctcttctg ctttgatcgg ttgctgaaca
    11401 gctttttcct gcggatgcgc catccagtac gcatgtagat caagcgccgc cagtcgaatg
    11461 gcatctacgc tctcttcaaa cgcttctggc gacattttct ctacccgctc gaagttttcc
    11521 tcagtaccgt gcagcgcaat cgcctccagc gctggtttta acgaatcggg caacgttgac
    11581 cagtcagaaa gtgccacgcc ccgcatatag ccaaagcacc actcctcaac aatcgtcagc
    11641 tcgctgccat caacttctcg caagccgaat aacggctcaa actgctccgg gaattcgttc
    11701 agacgctctg cggtatcggc catatgttga aaagccagat tcataaagcg cgtcatctct
    11761 ttctctgacg cccagcgcgg cacatagtca gccccacccc acacggcaac cagccactgt
    11821 tccggttcaa tctcttgcgg agaactcaac accgccgtca ataaaccgtc cagctccgcc
    11881 acatcaagga tggcgtggtc agtgttgtat ttggtcagaa tatcgtccag ccattccaac
    11941 tcactttcgt ttaacggtcc cgttttcata cgcttttcct tgtggatctc aactcgccag
    12001 cacctatctt acatgccggt ccgtatcaga gatacttttt gagtggcttt gctggtgatt
    12061 aaaaattagg gagggtgtaa cgacaagttg caggcacaaa aaaaccaccc gaaggtggtt
    12121 tcacgacact gcttattgct ttgattttat tcttatcttt cccatggtac ccggagcggg
    12181 acttgaaccc gcacagcgcg aacgccgagg gattttaaat cccttgtgtc taccgattcc
    12241 accatccggg ctcgggaaga aagtggaggc gcgttccgga gtcgaaccgg actagacgga
    12301 tttgcaatcc gctacataac cgctttgtta acgcgccaaa ttcttcaggc ctttcagcca
    12361 gacatccgct tgacgccgat gtcttttaaa ctggagcggg aaacgagact cgaactcgcg
    12421 accccgacct tggcaaggtc gtgctctacc aactgagcta ttcccgcatt catcaagcaa
    12481 tcagttaatc acttgatttt attatcgtct ggcaatcagt gccgccgttc gatgcgttgc
    12541 attctactta cctggcgcga tgagtcaacg atatttttca ccacttttga tcgtttgctg
    12601 aaaattacgc cgaaacgatc actgatcaag caaatctgca cgcgcagcgc tcaaatattg
    12661 caacattgac cacagagtca gtaccgcagc cacaaagaaa agtgcaatac cggcgtactc
    12721 aacccaaatg ttcggacgcc acagcagcca ggccaacgcc accatctggg cagtggtttt
    12781 cactttccca atccaggaga cagccacgct actgcgttta cccaactccg ccatccattc
    12841 gcgtagcgca gaaataataa tttcacgggc gatcatcgtt gccgccggta gggtcaccca
    12901 ccagctgtga taatgctcgg ttaccagcac catggcgata gccacgagaa ctttatctgc
    12961 tacagggtca aggaaagcac caaaccgggt actctggttc cagcggcgtg ccagaaaacc
    13021 atcgaaccag tcagtcaccg ccgcgacgca gaaaatgagc gcggcggcaa acggcgacca
    13081 ggtgacaggc agataaaaga ccaatacaaa gaatgggata aggatgacac ggaacagtgt
    13141 aagcaacgta gggatattaa attgcataat gacgggtaac tatctgttgt cagtaagatt
    13201 acccctatgt tgctacagag acatcaatgt ttcaacgacc agaagatctt ttctgccaga
    13261 ccttgcgaaa tacccggcac ttttgcaatt tcctcgacgc tggcgttacg taaaccttgc
    13321 aaaccgccca tatatttcaa caacatttgc cgacgttttg gcccgacgcc ttcaatggtt
    13381 tccagggagc tggtattttt gaccttcgcc cgttttttac ggtgcccgcc aatcgcgtga
    13441 tcgtgtgatt catcgcgaat atgctggata acatgcagcg cgggagaatc tggcggcaaa
    13501 ctgaatccct caccttccgg ctcaaagaac agtgtttcca gcccagcctt acgatctact
    13561 cctttggcaa cgccaagtag cagtggatga tttttatccc atgagacatc cagttcggcg
    13621 aagacatttt tcgcctgcgc aagctggcct ttgccgccgt cgataaggat cacatccggg
    13681 atcttactgt cgtcaatggc tttaccataa cgccgacgca gcacctgatt catcgccgca
    13741 taatcatcgc ccggcgtgat gcctgtaatg ttatagcgcc gatactccgc acgcagcggg
    13801 ccgttagcat caaacaccac acaggaagcg acggtttgtt cgcccatggt atggctgatg
    13861 tcaaagcact ccatccgctt cacttccggc aatttcaaca cgctggcaag cgcggtcagt
    13921 cgctggtgaa cggtagattg ctgcgaaagt ttgctggtta aggccgtcgc cgcattggtg
    13981 cgcgcgagtt tcagataacg cgccctatcg ccgcgaggtt tggtttgaac attaatcttg
    14041 cgtcccgcca gttctgaaag ggaatcggcg agcagcgttt tatcgctaag attaaaatcg
    14101 agcaggatct cacccggtaa ggtgcgcatc tggctgcctt gtaaatagaa ctggcctacg
    14161 aaggtttcta ccacctcgct cagttccgta ccgccaggca ctttcgggaa atagctgcgg
    14221 ctgccgagca ctttgccctg acgaatgaac aatacgtgga cacaagccat gcccgcatcg
    14281 aacgccacac caataacgtc gaggtcgtcg ccggtattgg aaacgaattg tttttcggtg
    14341 acgcgtcgca ccgcctgaat ttggtcgcga atacgtgcag cttcttcaaa ctccagattc
    14401 tggctggcag tttccatacg actaatgagt tgcgtaagca cctgatcatc tttgccagac
    14461 aaaaacaggc gcacatactc gacctgctga gcgtattctt cttcactcac cagtccttca
    14521 acgcacggtc ccagacagcg ccctatctgg tattgcagac acggacgcga gcgattgcga
    14581 taaacactat tttcgcactg gcgaatgggg aaaatctttt gcagtagcgc cagtgtttca
    14641 cgtacggcat agccattcgg gaacgggccg aaatattcac ctttggcatg cttcgcacca
    14701 cgatgcatcg ccagacgcgg gtgggtatcg ccactcagga agataaaagg ataagattta
    14761 tcatcgcgta gcaaaacgtt gtaacgcggc tgatagagtt tgatgtagtt gtgttccagc
    14821 aacagcgctt cggtttctgt atgagtaacc gttacatcaa tttgctggat ctgggcgacc
    14881 agcgcttcgg ttttgcgcga agcgaggttg ctacggaaat agctggaaag ccgttttttc
    14941 aggtctttcg ctttgccgac atagataacc gtaccaccag catcgtacat gcgataaacg
    15001 cctggctggc tggttacggt ttttaaaaac gcttttgcgt caaactgatc actcactgac
    15061 ttgataatgt ctccgcatta cacagaccat ggcgaattgc caggtgagtc agctcaacat
    15121 cgccatgaat gtttagttta ctgaacatac gatagcggta gctgttcacc gttttcggac
    15181 tgagattgag ctgttctgag atctcattga ccttctggcc cttggtgatc atcagcataa
    15241 tctgcaattc acgttcagac aaactggcaa atgggctttc tgttttttct ggttcgatct
    15301 ggcttaacgc catttgttga gcgatgtcag aagcaatgta acgctgccct gaatagacag
    15361 aacgaatcgc actcacgact tcctgcggag ccgcgccttt gctgaggtag cccgcagcac
    15421 cggcctgcat gactttcgct ggtaaagggt tttctgtatg gacggtaagc atgatgattt
    15481 tgacatcagc tgtggaacgc gcgattttac gcgtcgcctc aagaccgcca atgcccggca
    15541 tactcatgtc cattagcacc acgtcaacgg catttgcccg acaccactta acggcgtctt
    15601 caccgcacga tgcctcaccg acgactttta taccctttat atcttccaga atgcgtcgta
    15661 tccctgcgcg caccagttcg tggtcatcaa caagtagaac gttgatcaaa ggaatatctc
    15721 cagaaatagg gataacgcta ctgatagtta gtcattcgta tattagcggt ttttattgca
    15781 actttgaaac gttaaaaatg gtgggtattc gatttttctc tcgtttttgt caattcgatt
    15841 gtccacagtt atggtcacgc ccgtcaccaa cggcttaccg gatcgtttca cagtaagccc
    15901 tgcgacgaaa tgtgacaaaa attaccttta ttcagcaaaa atgaaaatca gcccgctgaa
    15961 tatgtaacat taattaaccc gaacaatacg ggc
//
LOCUS       DAZFWL020000103        15948 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFWL020000103 DAZFWL020000000
VERSION     DAZFWL020000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.103"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..79)
                     /gene="yojO"
                     /locus_tag="ACHKNP_RS17620"
                     /old_locus_tag="pgaptmp_003524"
     CDS             complement(<1..79)
                     /gene="yojO"
                     /locus_tag="ACHKNP_RS17620"
                     /old_locus_tag="pgaptmp_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HGU4705624.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFA"
     gene            188..682
                     /gene="napF"
                     /locus_tag="ACHKNP_RS17625"
                     /old_locus_tag="pgaptmp_003525"
     CDS             188..682
                     /gene="napF"
                     /locus_tag="ACHKNP_RS17625"
                     /old_locus_tag="pgaptmp_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HGU4705625.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            672..935
                     /gene="napD"
                     /locus_tag="ACHKNP_RS17630"
                     /old_locus_tag="pgaptmp_003526"
     CDS             672..935
                     /gene="napD"
                     /locus_tag="ACHKNP_RS17630"
                     /old_locus_tag="pgaptmp_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HGU4705626.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            932..3418
                     /gene="napA"
                     /locus_tag="ACHKNP_RS17635"
                     /old_locus_tag="pgaptmp_003527"
     CDS             932..3418
                     /gene="napA"
                     /locus_tag="ACHKNP_RS17635"
                     /old_locus_tag="pgaptmp_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HGU4705627.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3425..4120
                     /gene="napG"
                     /locus_tag="ACHKNP_RS17640"
                     /old_locus_tag="pgaptmp_003528"
     CDS             3425..4120
                     /gene="napG"
                     /locus_tag="ACHKNP_RS17640"
                     /old_locus_tag="pgaptmp_003528"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HGU4705628.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4107..4970
                     /gene="napH"
                     /locus_tag="ACHKNP_RS17645"
                     /old_locus_tag="pgaptmp_003529"
     CDS             4107..4970
                     /gene="napH"
                     /locus_tag="ACHKNP_RS17645"
                     /old_locus_tag="pgaptmp_003529"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HGU4705629.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGYICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4967..5416
                     /gene="napB"
                     /locus_tag="ACHKNP_RS17650"
                     /old_locus_tag="pgaptmp_003530"
     CDS             4967..5416
                     /gene="napB"
                     /locus_tag="ACHKNP_RS17650"
                     /old_locus_tag="pgaptmp_003530"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HGU4705630.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5426..6028
                     /gene="napC"
                     /locus_tag="ACHKNP_RS17655"
                     /old_locus_tag="pgaptmp_003531"
     CDS             5426..6028
                     /gene="napC"
                     /locus_tag="ACHKNP_RS17655"
                     /old_locus_tag="pgaptmp_003531"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HGU4705631.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6041..6664
                     /gene="ccmA"
                     /locus_tag="ACHKNP_RS17660"
                     /old_locus_tag="pgaptmp_003532"
     CDS             6041..6664
                     /gene="ccmA"
                     /locus_tag="ACHKNP_RS17660"
                     /old_locus_tag="pgaptmp_003532"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HGU4705632.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6661..7323
                     /gene="ccmB"
                     /locus_tag="ACHKNP_RS17665"
                     /old_locus_tag="pgaptmp_003533"
     CDS             6661..7323
                     /gene="ccmB"
                     /locus_tag="ACHKNP_RS17665"
                     /old_locus_tag="pgaptmp_003533"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HGU4705633.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VSGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7365..8102
                     /gene="ccmC"
                     /locus_tag="ACHKNP_RS17670"
                     /old_locus_tag="pgaptmp_003534"
     CDS             7365..8102
                     /gene="ccmC"
                     /locus_tag="ACHKNP_RS17670"
                     /old_locus_tag="pgaptmp_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HGU4705634.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8099..8308
                     /gene="ccmD"
                     /locus_tag="ACHKNP_RS17675"
                     /old_locus_tag="pgaptmp_003535"
     CDS             8099..8308
                     /gene="ccmD"
                     /locus_tag="ACHKNP_RS17675"
                     /old_locus_tag="pgaptmp_003535"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HGU4705635.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8305..8784
                     /gene="ccmE"
                     /locus_tag="ACHKNP_RS17680"
                     /old_locus_tag="pgaptmp_003536"
     CDS             8305..8784
                     /gene="ccmE"
                     /locus_tag="ACHKNP_RS17680"
                     /old_locus_tag="pgaptmp_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HGU4705636.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8781..10724
                     /gene="ccmF"
                     /locus_tag="ACHKNP_RS17685"
                     /old_locus_tag="pgaptmp_003537"
     CDS             8781..10724
                     /gene="ccmF"
                     /locus_tag="ACHKNP_RS17685"
                     /old_locus_tag="pgaptmp_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HGU4705637.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10721..11278
                     /gene="dsbE"
                     /locus_tag="ACHKNP_RS17690"
                     /old_locus_tag="pgaptmp_003538"
     CDS             10721..11278
                     /gene="dsbE"
                     /locus_tag="ACHKNP_RS17690"
                     /old_locus_tag="pgaptmp_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HGU4705638.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11275..12327
                     /gene="ccmH"
                     /locus_tag="ACHKNP_RS17695"
                     /old_locus_tag="pgaptmp_003539"
     CDS             11275..12327
                     /gene="ccmH"
                     /locus_tag="ACHKNP_RS17695"
                     /old_locus_tag="pgaptmp_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HGU4705639.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQENK"
     gene            complement(12362..13009)
                     /gene="narP"
                     /locus_tag="ACHKNP_RS17700"
                     /old_locus_tag="pgaptmp_003540"
     CDS             complement(12362..13009)
                     /gene="narP"
                     /locus_tag="ACHKNP_RS17700"
                     /old_locus_tag="pgaptmp_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HGU4705640.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13329..15920
                     /gene="yejO"
                     /locus_tag="ACHKNP_RS17705"
                     /old_locus_tag="pgaptmp_003541"
                     /pseudo
     CDS             13329..15920
                     /gene="yejO"
                     /locus_tag="ACHKNP_RS17705"
                     /old_locus_tag="pgaptmp_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         3535 a         3929 c         4657 g         3827 t
ORIGIN      
        1 gagcaaaagc ccgtttttgc cacgcttccc gccactcttt tgatcctgct acaggtttta
       61 ccccgatcgg ggtatgcatc tttgacacat cctttaatat cttagcggct ataaaaatgg
      121 cttattaatt atgcggttta tttggtcgct ctcaattttc agagcgcgtt aatgatggaa
      181 ggtcaatgtg aagattgatg catcccgtcg gggcatactc actggtcgct ggcgcaaagc
      241 cagtaacggt atccgtccgc cctggtccgg tgatgaatct cattttctga cccattgcac
      301 ccgctgcgac gcctgtatta atgcttgtga aaacaacatt ctgcaacgcg gcgcgggcgg
      361 ctatccgagc gttaatttca aaaataacga gtgcagcttc tgttacgcct gcgcgcaggc
      421 ttgccccgaa tcgctatttt ctccgcgcca caccagggcc tgggatttgc agttcaccat
      481 tggggacgcg tgcctggcgt atcagtcagt tgaatgccgc cgctgtcagg atagctgtga
      541 gccaatggca attatctttc gcccgacgct ctccgggatc taccagccgc aacttaatag
      601 ccaactttgc aacggatgtg gagcatgtgc agccagctgt ccggtatcag ccataactgc
      661 ggagtatctc catgcacact aactggcaag tttgcagcct ggtcgtgcag gccaaaagcg
      721 aacgaatttc agacatcagc acccaactga acgcctttcc cggctgtgaa gttgctgtca
      781 gcgacgcgcc gagcggtcag ttgattgtgg tggtggaagc agaagacagc gaaacgctga
      841 tccaaaccat tgagtcagta cgcaacgtag agggcgtgct ggcggtgtcg ctggtttatc
      901 accagcagga agagcaaggt gaggaaacac catgaaactc agtcgtcgta gctttatgaa
      961 agctaacgcc gttgcggccg ctgcggcggc tgccggtctc agcgtgccgg gcgttgcccg
     1021 cgccgttgtt ggtcagcagg aagccattaa atgggataaa gcgccgtgcc gtttctgcgg
     1081 tactggttgc ggcgttctgg tcggaacgca gcaggggcgt gtggtggcct gtcagggcga
     1141 cccggacgca ccggttaacc gtggcctgaa ctgcattaag ggctatttcc tgcccaagat
     1201 catgtacggt aaagaccgtt tgacgcagcc gctgctgcgt atgaaaaacg gtaaatatga
     1261 caaagaaggc gaatttaccc caatcacctg ggatcaggcc ttcgatgtga tggaagagaa
     1321 attcaaaacc gccctgaaag aaaaaggacc ggaatcgatc ggtatgttcg gttctggtca
     1381 gtggactatc tgggaaggtt atgccgcgtc caagctgttc aaagcgggct tccgttcgaa
     1441 caacatcgac ccgaacgcac gtcactgtat ggcgtcggca gtagttggct ttatgcgtac
     1501 ctttggtatg gatgagccga tgggctgcta tgacgacatc gagcaggctg acgcgtttgt
     1561 gctgtggggc gctaacatgg cggagatgca cccgatcctc tggtcacgca tcactaaccg
     1621 tcgtctctct aaccagaacg tcaccgtggc ggtgctttct acctaccagc atcgtagctt
     1681 cgagctggcg gataacggca tcatctttac gccacagtct gacctggtga tcctgaacta
     1741 catcgccaac tatatcattc aaaacaatgc gataaatcag gacttcttca gcaagcacgt
     1801 taacctgcgc aaaggggcga cggacatcgg ctacggttta cgtccgaccc atccgctgga
     1861 aaaagcagcg aagaatccgg gttctgacgc ctccgaaccg atgagctttg aagattacaa
     1921 agccttcgtt gccgagtata cgctggaaaa aactgccgaa atgaccggcg tgccgaaaga
     1981 ccagttagaa caactggcgc agctgtatgc cgatccgaac aagaaagtca tctcctactg
     2041 gacgatgggc ttcaaccagc atactcgtgg cgtgtgggcc aacaacctgg tctacaacct
     2101 gcacctgctg accggcaaaa tttcccagcc gggttgcggt ccgttctccc tgaccgggca
     2161 gccttccgcg tgtggtactg cgcgtgaagt gggcaccttt gctcaccgtc tgcctgcgga
     2221 catggtggtg actaacgaga aacaccgtga tatttgcgag aagaagtgga atatcccgag
     2281 cggcaccatt ccggcgaaaa tcggtctgca cgcggtggca caagaccgtg cactgaaaga
     2341 cggcaagctg aatgtttact ggaccatgtg taccaacaac atgcaggccg ggccgaacat
     2401 taatgaagag cgtatgccgg gctggcgcga tccgcgcaac ttcatcatcg tctccgatcc
     2461 gtatccgaca gtcagtgcgc tggcagccga cttgatcctg ccgaccgcaa tgtgggtaga
     2521 gaaagagggc gcttacggta acgccgaacg ccgtactcag ttctggcgtc agcaggtaca
     2581 ggcgccaggc gaagcgaaat cggatctctg gcagttagtc cagttctccc gccgcttcaa
     2641 aactgaagaa gtatggccgg aagagctgct ggcgaagaaa ccggaactgc gtggcaaaac
     2701 gctgtacgaa gttctgtatg ccacccccga agtgagcaaa taccctgtat ccgaactggc
     2761 ggaagatcag ctgaacgatg aatcccgcga gctgggcttc tatctgcaaa aagggctgtt
     2821 cgaagagtac gcatggtttg gtcgcggtca cggtcacgat ctggcaccgt tcgatgacta
     2881 ccacaaagcg cgcggtctgc gctggccggt ggtgaacggt aaagaaacgc agtggcgtta
     2941 cagcgaaggt aacgacccgt acgtgaaagc gggcgaaggc tataagttct acggtaaacc
     3001 ggatggcaaa gcggtgatct tcgcactgcc gttcgaaccg gcggcggaag caccggatga
     3061 agagtacgac ctgtggctct ctaccggacg cgttctggag cactggcaca ccggcagtat
     3121 gactcgccgt gtgccggaac tgcaccgcgc cttcccggaa gcggtcttgt ttattcaccc
     3181 gctggatgcg aaagcgcgcg atctgcgccg tggcgacaaa gtgaaagtgg tttctcgccg
     3241 tggcgaagtg atctcgattg ttgaaacgcg cggtcgtaac cgtccgccac agggcctggt
     3301 gtacatgccg ttcttcgacg ccgcacagct ggttaacaaa ctgacgctgg atgcgaccga
     3361 tccgctctcg aaagagacgg acttcaagaa gtgcgcagtc aaactggaga aggtgtaagc
     3421 cattatgtcc cggtcagcga aacctcaaaa tggtcgccgc cgctttctgc gcgatgtcgt
     3481 tcgcacagca ggcgggctgg ctgccgtggg tgtggcgctg gggttacaac agcaaaccgc
     3541 acgcgcatct ggcgtgcggt tgcgcccgcc cggagccata aacgagaacg cctttgccag
     3601 tgcctgtgtg cgttgcggtc agtgtgttca ggcttgccct tacgacacct taaaactggc
     3661 gacgctggcc tctggtctgt cggcgggcac gccatatttt gtcgcacggg atattccttg
     3721 cgaaatgtgt gaggacattc cgtgcgccaa agtgtgccca agcggtgcgc tggatcgtga
     3781 gattgaatcg atcgacgacg cgcggatggg gctggcggta ctggtggacc aggaaaactg
     3841 tctcaacttt caggggctgc gctgcgatgt ttgttatcgc gaatgcccga aaattgatga
     3901 ggccatcacc ctggagctgg agcgcaacac gcgtaccggt aagcacgccc gctttctgcc
     3961 gacggttcac agcgacgcct gtaccggttg cggtaagtgc gaaaaagtat gcgtgctgga
     4021 acaaccggca atcaaggtgt taccgctgtc actggcgaaa ggggagttag gtcaccatta
     4081 ccgcttcggt tggctggagg ggaacaatgg caaatcgtaa acgtgacgcc gggcgcgagg
     4141 cgctggagaa aaaaggctgg tggcgcagtc accgttggct ggtgttacgt cgcctttgtc
     4201 agttcttcgt gctggggatg tttttgagcg gtccatggtt tggtgtgtgg atcttgcacg
     4261 gcaactacag cagtagcctg ttattcgaca ccgttccgct gaccgatccg ctgatgacgc
     4321 tgcaaagtct tgccagtggt catctgcctg ccacggtggc gttgaccggt gcggtgatta
     4381 tcaccgtgct ctacgccctg gcgggaaagc gattattttg cagctgggtc tgcccgctga
     4441 acccgattac cgacttagcg aactggttac gcaggcgctt tgacctcaat cagtctgcga
     4501 cgatccctcg ccacattcgc tacgtgctgt tggtggtcat tctgctggga tcggccttaa
     4561 ctggcacgct catctgggaa tggatcaacc ccgtatctct gatggggcgc agcctggtaa
     4621 tgggcttcgg cagcggcgcg ctgcttattc tcgcgctgtt tttatttgat ctactggtcg
     4681 ttgaacacgg ctggtgcggg tacatttgtc cggttggcgc gctgtatggc gtgctgggta
     4741 gcaaaggtgt gattaccgtt gcggccaccg atcggcagaa atgtaaccgc tgtatggatt
     4801 gttttcatgt ttgcccggaa ccgcatgtgc tacgtgcccc ggtgctggat gaacaaagcc
     4861 cggtgcaggt caccagccgc gattgcatga cgtgcggtcg ctgcgtggat gtctgttctg
     4921 aggatgtatt tacaataact acacgatgga gttcgggagc gaaatcatga aaagccatga
     4981 cctgaagaaa gcgctgtgtc aatggacggc gatgctggcc ctggtggtaa gcggcgcggt
     5041 ttgggcggct aatggcgtcg attttagcca gtcgccggaa gtatccggga cacaggaagg
     5101 ggccattcgg atgccaaaag agcaggaacg gatgccgctg aactatgtga atcagccgcc
     5161 aatgatcccg catagcgttg aaggttatca ggtaacgacc aataccaacc gctgcttgca
     5221 gtgccacggt gtcgaaagtt atcgcaccac tggcgcgccg cgtatcagtc ctactcactt
     5281 tatggacagc gacggcaaag tgggcgcgga agtggctccg cgtcgttatt tctgtctgca
     5341 atgtcacgta ccgcaggccg ataccgcgcc aatcgtgggg aataccttta ccccatcaaa
     5401 aggttacggg aaataagagg ttattatggg aaattctgac cgtaagcctg gtctgattaa
     5461 gcgcctgtgg aaatggtggc gtacccccag ccgtctggcg ctggggacgc tgctgttgat
     5521 cggttttgtt ggcggcatcg tcttctgggg tggctttaac accgggatgg aaaaagccaa
     5581 taccgaagag ttctgcatta gctgccacga aatgcgcaac acggtgtatc aggaatacat
     5641 ggattccgtg cactacaaca accgtagcgg cgtccgtgcg acctgtccgg attgtcacgt
     5701 tccgcacgag tttgtgccga agatgatacg caagctcaaa gcaagtaaag agctgtatgg
     5761 taaaattttt ggcgttattg acacgccgca gaaatttgaa gctcatcgtc tgacgatggc
     5821 acagaatgag tggcggcgca tgaaggacaa taactcgcag gagtgccgta actgtcacaa
     5881 cttcgagtat atggatacaa ccgcccagaa atcggttgcc gcgaagatgc atgaccaggc
     5941 ggtgaaagat gggcaaacct gtattgattg ccataaaggg atagcgcaca agctgcccga
     6001 tatgcgtgaa gtcgagccag gtttttaaca gggttattgc gtgggtatgc ttgaagccag
     6061 agagttactt tgtgagcggg atgaacgaac cttatttagt ggcttgtcat ttacgctgaa
     6121 cgcaggagag tgggtacaaa tcaccggtag caacggcgcg gggaagacaa cgcttctccg
     6181 tttgctgacg gggttgtctc gccctgacgc aggcgaggtt ctctggcaag ggcagccctt
     6241 gcatcaggta cgcgacagct accatcaaaa cctgttatgg ataggccatc agccggggat
     6301 caaaacccgg ctgacggcgt tagaaaatct gcacttttat catcgcgatg gcgataccgc
     6361 acaatgtctg gaagccctgg cgcaggccgg gcttgccgga ttcgaagata ttcctgtaaa
     6421 tcagctctcg gccgggcaac aacgccgcgt cgctttagcg cgtctgtggc tgacccgtgc
     6481 cacgttatgg atcctcgacg agccttttac cgcgattgac gttaacggcg tcgatcgtct
     6541 gacccagcgt atggcgcagc atacggagca gggggggatt gtgattctga ctacccacca
     6601 gccgctcaac gttgctgaaa gtaaaattcg ccgcatttca ctgacgcaaa cgagggccgc
     6661 atgatgttct ggcgcatttt ccgtcttgag ctgcgtgtag cgtttcgcca tagcgccgaa
     6721 atcgccaacc cgctgtggtt cttcctgatt gtaattaccc tttttccgct cagtatcggt
     6781 ccggagccgc aactgctggc gcgtattgca ccgggcatta tctgggttgc tgcactgctt
     6841 tcatccttgc tggcgctgga acgactgttc cgtgacgatt tgcaggacgg cagtcttgaa
     6901 caattgatgt tgttgccgtt acccttgccc gccgttgtgc tggcgaaggt gatggcgcac
     6961 tggatggtaa gcggtctgcc gttactcatt ctttcgccac tggtagcaat gctactggga
     7021 atggatgttt atggctggca agtgatggcg ctgacgctgc tgctgggaac gcctacgctt
     7081 ggctttctcg gtgcaccggg cgtggcgctg acagtgggac ttaagcgcgg tggtgtgctg
     7141 ctcagcatac tggtgttacc gctgactatc ccattactca tcttcgccac cgccgcgatg
     7201 gacgcggctt ccatgcattt gcccgttgac gggtatctgg caattttagg cgcgttgctg
     7261 gcaggcaccg cgacattaag tccttttgcg acggcggcag cgttacgaat cagcattcaa
     7321 taacgctgga ttactgattt ttgttcgagt ctggtatcga aactatgtgg aaaacactgc
     7381 atcaactggc gatcccacca cggctgtatc aaatctgtgg ctggtttata ccgtggctgg
     7441 caattgccag tgtggtcgtg cttaccgtcg gctggatctg gggattcggc tttgctccgg
     7501 ctgattatca gcagggaaat agctaccgca ttatctacct gcatgtgcct gcggcgatct
     7561 ggtcgatggg catttatgca tcaatggcag tggcagcgtt tattggcctt gtctggcaga
     7621 tgaaaatggc caacctggcg gtggcggcga tggcccccat tggtgccgtg tttaccttta
     7681 ttgccctggt taccggctct gcatggggaa aaccgatgtg gggcacctgg tgggtatggg
     7741 atgcacgtct gacttctgaa ctggtgctgc tttttttgta tgtgggtgtg attgccctgt
     7801 ggcacgcctt cgacgaccgc cgtctggcgg gccgtgcggc aggtatcctg gtgctgattg
     7861 gcgtggtgaa tctgccgatt attcattact ccgtggagtg gtggaacacc ctgcatcagg
     7921 gatcaacgcg gatgcagcaa agtatcgatc cggcgatgcg ttcgccgctg cgctggtcga
     7981 tttttggctt cctgctcctg tctgccacgc tgacgctgat gcggatgcgt aatttgattt
     8041 tgctgatgga aaaacgccgt ccgtgggtga gtgaactgat actgaaaaga ggccgtaaat
     8101 gacccctgca tttgcttcct ggaatgaatt tttcgcaatg ggcggttacg ccttttttgt
     8161 ctggctggcg gtggtgatga ccgttattcc gctggtggtt ttggtcgtgc actcggtgat
     8221 gcagcatcgc gcaattctgc gtggtgtggc gcaacagcgg gcgcgtgagg cgcgtttacg
     8281 tgctgcgcaa cagcaggagg ctgcatgaat attcgccgta aaaaccgctt gtggattgcc
     8341 tgtgctgttc tggcagggtt ggcactgact atcggtctgg tgctatatgc gctgcgctcg
     8401 aatatcgatc tcttttatac gccgggggaa attctctacg gcaagcgtga aactcagcaa
     8461 atgccggaag tcggtcagcg tctgcgcgtt ggcgggatgg tgatgccggg tagcgtgcag
     8521 cgcgatccca attcgctaaa agtaactttc accatttacg acgctgaagg ttcagtagat
     8581 gtctcttacg aaggcatttt gccggatctg ttccgtgaag ggcagggcgt tgtggtgcag
     8641 ggcgaactgg aaaaaggcaa tcatatcctc gcaaaagaag tgctggcgaa acacgacgaa
     8701 aactacacgc cgccagaagt tgagaaagcg atggaagcca atcaccgtcg cccggcgagt
     8761 gtttataagg acccagcatc atgatgccgg aaattggtaa cgggctgctg tgtctggcgc
     8821 taggaattgc gctgctgctg tccgtgtatc cgctatgggg cgtagcgcgc ggagatgcgc
     8881 gcatgatggc gtcttcccgc ttgtttgcct ggctgctgtt tatgtctgtg gctggcgcat
     8941 ttctggtact ggtcaatgcc ttcgtggtca acgacttcac cgtcacctat gttgccagca
     9001 actccaatac ccagcttccg gtgtggtatc gcgtggcggc tacctggggc gcgcatgaag
     9061 gctcgctcct gctgtgggtg ctgctgatga gcggctggac ctttgcggta gcgattttta
     9121 gtcagcgtat tccgctggat attgtggccc gcgtactggc gataatgggg atggtcagcg
     9181 tcggcttttt gctgttcatt ctctttacct ctaacccgtt ctcacgcacg ttgccgaact
     9241 tcccgattga agggcgcgat cttaacccgc tgttacagga tccggggctg atcttccatc
     9301 cgcctctgct ctatatgggg tacgtggggt tctcggtggc gtttgctttt gccattgctt
     9361 ctttgttgtg cgggcgtctg gacagcactt atgcgcgttt tactcgtccg tggacgctgg
     9421 cggcgtggat cttcttgacg ctcggcattg tgctcggttc cgcatgggcc tattacgaac
     9481 tcggctgggg cggctggtgg ttctgggatc cggtagaaaa cgcctcgttt atgccgtggc
     9541 tggtggggac tgcgctgatg cactcactgg cggtcactga acaacgcgcc agcttcaaag
     9601 cgtggacatt actgctggca atcagtgcct tctcgttgtg tctgctgggg actttcctgg
     9661 tgcgttccgg cgtgctggta tcggtacacg cgtttgcctc tgatccggca cgcggtatgt
     9721 ttatcctcgc ctttatggta ctggtgattg gtggttcgct gctgctgttt gccgcgcgtg
     9781 gacacaaagt tcgctcacgc gtaaacaatg cgctgtggtc gcgggaatct ctgctgttag
     9841 cgaacaacgt tttgctggtc gccgcgatgc tggtggtatt actggggacg ctgctgccgc
     9901 tggtgcacaa gcaactggga ctgggcagta tttcgattgg cgaaccgttc ttcaacacca
     9961 tgtttacctg gctgatggtg ccgtttgcgc tgctgcttgg tgtcggtcct ctggtgcgct
    10021 gggggcgcga tcgcccacgt aaaatccgca atttattgat tatcgccttc atctcaacgc
    10081 tggtgctgtc gctgctgctg ccgtggctgt tcgaaagcaa agttgtggcg atgacggtgc
    10141 tcggcctggc aatggcctgc tggattgctg tgctggcaat tgcggaagct gcgctgcgta
    10201 tttcacgtgg cacgaaaacc accttcagtt attggggaat ggtggcggct cacctggggc
    10261 tggcagtgac aattgttggt attgccttta gccagaacta tagcgttgag cgtgatgtgc
    10321 gcatgaagtc cggcgatagc gtcgatattc atgaatatcg cttcaccttc cgtgatgtca
    10381 aagaggtgac tggcccgaac tggcgtggcg gtgtggcgac tatcggcgta acgcgcgatg
    10441 gcaagccgga aacggtgctg tatgcggaaa aacgttatta caacactgcc gggtcgatga
    10501 tgaccgaagc ggcaattgac ggcggcatca cgcgtgacct gtacgccgcg ctcggtgaag
    10561 agctggaaaa cggcgcgtgg gccgtgcgtc tttactacaa accatttgtt cgctggattt
    10621 gggcgggcgg gctgatgatg gcgttgggcg gactactgtg tctgtttgat cctcgctatc
    10681 gtaagcgcgt gagtccgcaa aaaactgcgc cggaggccgt atgaagcgca aagtattgtt
    10741 aattccgttg attatcttcc tggcgattgc cgcggcgctg ctgtggcagc tggcgcgtaa
    10801 tgccgaaggg gatgatccga ccaatctgga atcggcgctc attggcaagc ctgtgccgaa
    10861 gtttcgtctc gaatcactgg acaatccggg gcagttttat caggcggatg tgctgactca
    10921 gggcaaacca gtactgctta acgtctgggc gacctggtgt ccgacctgcc gtgcggaaca
    10981 tcaatatctg aatcagcttt cagcgcaggg cattcgcgtg gtcggcatga actataaaga
    11041 cgatcgccag aaggcaatca gctggctgaa agagctgggc aatccttacg cgctaagcct
    11101 gtttgatggc gacggcatgt tagggctgga tctcggtgtc tatggcgcgc cagaaacgtt
    11161 ccttattgac ggcaacggca tcattcgcta tcgccatgcg ggcgatctca atcctcgcgt
    11221 ctgggaagaa gagatcaagc cgctgtggga gaaatacagt aaggaggccg cacaatgagg
    11281 tttttattgg gcgtgctgat gctgatgatc tccggctcag cgctggcgac catcgacgtg
    11341 ttgcagttta aagatgaagc acaggaacaa cagttccgtc agctcactga agaactgcgc
    11401 tgcccgaaat gccagaataa cagcattgcc gattccaact cgatgattgc caccgacctg
    11461 cgccagaaag tgtatgaact gatgcaggaa ggtaaaagta agaaagagat tgtcgattat
    11521 atggtggcgc gttacggcaa cttcgtcact tacgatccgc cgttaacgcc gctgaccgtg
    11581 ctgctgtggg tgctgccagt agtggctatt ggcattggcg gttgggtcat ttacgcccgc
    11641 tcgcggcgtc gggtacgcgt ggtgccggaa gcgtttcctg aacaaagcgt gccggaaggt
    11701 aagcgtgccg gatatgtcgc ttatttgccg ggcattgtgg tggcgttaat tgtggctggc
    11761 gtcagctact accagaccgg aaattatcaa caggtgaaaa tctggcagca ggccacggca
    11821 caggctccgg cgttgctgga cagggcgctg gatccgaaag ccgatccgct caacgaagaa
    11881 gagatgtcgc gtcttgcgct ggggatgcgt actcaactgc aaaaaaatcc gggagatata
    11941 gaaggctgga ttatgttggg ccgcgttggc atggcgctgg gtaacgctag tatcgccacc
    12001 gatgcatacg ctactgcgta tcgcctcgat ccgaaaaaca gcgatgccgc actgggttat
    12061 gctgaagcgt tgacacgttc atctgatccc aacgacaacc gcctcggcgg cgaactgctg
    12121 cgccagttgg tgagaagcga ccacagcaat atccgtgtgt taagcatgta tgcgtttaat
    12181 gcctttgagc agcagcgatt tggcgaagcc gttgccgcgt gggagatgat gttgaaactc
    12241 ttacctgcca acgatactcg ccgtgcggtg attgaacgta gtatcgcgca ggcgatgcaa
    12301 catttgtcgc cgcaggagaa taaataaaaa gaggcccgat ggtgatgcca tcgggctatt
    12361 tttattgtgc cccgcgttgt tgcaggaaca gaatggtggc tgccacgcgt gagcggacat
    12421 tgagtttgcg cagcagattg cgaatatgta cttttactgt ctgctctgaa atattcaaca
    12481 ccgaggcaat ctgtttattt gacagcccct gtgccagctc gtgcagaaca tccagctcgc
    12541 gctccgtcag cacgctgaag ggatcttctt ccgcgccaaa catttcacgt tcacgtaagt
    12601 actgattgac gcgttcgcta aagactttgc tgcctttcgc tccagcacga attgcttcca
    12661 gcaatacttc cgggtcgctg tctttcaaca gataaccgtc tgcgcctgcg tctatcagcg
    12721 caaagacatc gctggaggca tctgatacgg tcaggataat aatttgcgcg gtaacgccat
    12781 ccctgcgcaa ggcattgagg gtatccaggc cactcatacc tttcatattg agatccagca
    12841 agatcacgtc gatatccagt ctattcgcca gatcgatcgc gctcgcgccg tcgcccgctt
    12901 cggcgaccac ttcaaagcca ggatcaagct ccagtaactg acgaacaccg cgtcgcataa
    12961 gtggatgatc atccacaatc atcacctgaa aaggcgttgc ttcaggcata gtagtctcct
    13021 gaggttttat tagaatcatt atgtttgagc gtagcgttgg taacgctacc aggcgcgtga
    13081 atgttacccc attttatgta tggacatgta gtgaaaacta caaccttgtc gttcgctttg
    13141 gcatcatttt cattataaag acggaatttt gcatcagctt ctcatcaaga aaaacaacta
    13201 aaaaatcaat cacaatactt gtgcatttct ttaaacagtt tttaaaaact gactcccggt
    13261 atggagctat gggtattttc tgtacccaat gcttttaaca gcaattaatt tcataggatg
    13321 aaagctcaat gcatcaatct ggttctgttt ctctttgtcg ttccgcaata tctgttctgg
    13381 tggctacagc gttatattca cccatagcat tggcatcaac agttgagtat ggtgagacag
    13441 ttgatggtgt tgtcctggaa aaagatatcc agctggttta tgggaccgcc aataatacga
    13501 aaatcaatcc tggcggagaa cagcatataa aagaatttgg tgtaagtaat aatactgaaa
    13561 ttaacggagg gtatcagtac attgaaatga atggcgccgc agaatactca gtattaaatg
    13621 acggttatca aattgttcaa atgggtggcg cggcaaacca gactacgctc aataatggtg
    13681 tgctacaggt ttatggcgca gcgaatgata ccacgattaa aggcgggcgc ttaatcgttg
    13741 aaaaagatgg gggggccgtc tttgtcgcta tcgaaaaggg aggactactg gaggttaaag
    13801 aggggggatt agcgattgcg gtagatcaga aagcaggcgg tgctattaaa acaaccacgc
    13861 gggccatgga ggtattcgga acaaaccgtc tcggtcagtt cgatatcaag aatggtattg
    13921 ctaataatat gttgttggaa aacggcggaa gtttgcgagt tgaagaaaat gacttcgctt
    13981 ataataccac tgtagatagt ggcggcttac tggaggttat ggatggcggg actgtaactg
    14041 gcgttgataa aaaagcaggc ggaaaattaa ttgtctcaac gaatgcgctg gaagtgagtg
    14101 gtccaaacag taaaggccaa tttagtataa aagatggtgt gtcaaaaaat tatgaactgg
    14161 atgatggttc cgggctcatt gttatggagg acacgcaggc cattgatact atccttgatg
    14221 agcatgccac tatgcaatcg ctgggaaagg atactggtac gaaagtgcag gcaaatgcgg
    14281 tatatgatct cggtcgatca tatcagaatg gaagtatcac gtattcctca aaagccatct
    14341 ctgaaaatat ggttatcaac aatggccgcg ctaacgtctg ggctggcaca atggttaacg
    14401 tttcagtcag agggaatgat ggcattcttg aggtcatgaa gccgcaaata aattatgcac
    14461 ccgcaatgtt ggtgggtaag gtagtggttt ctgagggcgc ttcttttaga acgcatagtg
    14521 ccgtggatac cagcaaagcg gacgtttcgc tcgaaaatag cgtatggacc atcattgccg
    14581 atatcactac gacgaaccaa aacaccctcc tcaacttagc caaccttgcg atgtctgacg
    14641 caaatgtgat tatgatggat gagccagtga ctcgttcatc agtgacggca agtgcggaaa
    14701 atttcattac gttgaccacc aataccctgt cgggaaacgg caatttttat atgcgtaccg
    14761 atatggctaa tcatcagagc gatcagctca acgtcaccgg tcaggcaaca ggtgatttca
    14821 aaatattcgt gacggacacc ggtgccagcc cggcagcagg agatagcctt acactggtaa
    14881 caacgggcgg cggtgatgct gcatttacgt tgggcaatgc cggaggcgtt gttgatatcg
    14941 gtacgtatga atataccttg ctggataatg gcaaccatag ctggagtttg gcagagaatc
    15001 gcgcgcaaat taccccttca accactgatg tgctgaatat ggcggcagca caaccgctgg
    15061 tatttgatgc agaactggac accgtgcgtg agcgtcttgg tagcgtaaaa ggcgttaatt
    15121 acgatacggc gatgtggagt tcggcaatta acacccgcaa caacgtgacc actgatgcgg
    15181 gcgctggctt tgagcaaaca ttgaccgggc tgacgctcgg tatcgatagc cgtttctccc
    15241 gtgaagaaag cagcacaatt cgcggcttgt tctttggtta ctctcattct gatattggtt
    15301 ttgatcgcgg cggcaaaggc aatgtcgata gctataccct gggggcttat gccggttggg
    15361 agcatcagaa cggtgcctat gttgatggag tggtgaaagt tgaccgtttt gccaacacca
    15421 tccatggcaa gatgagtaat ggggcaacag cgtttggcga ttacaatagt aacggcgcgg
    15481 gtgctcatgt cgagagcggg ttccgttggg ttgacggatt gtggagtgtt agaccctatc
    15541 tggcctttac cggctttacc acagatggtc aggactacac gttatcaaac ggcatgcgcg
    15601 cggatgtggg aaatacccgg atattacgcg ctgaagcggg aacggcggta agctatcaca
    15661 tggacctgca aaacggtacg acgctggaac cctgactgaa agccgccgtg cgtcaggaat
    15721 acgccgattc taaccaggtg aaagttaatg acgatggcaa atttaataat gatgtggctg
    15781 gaacccgtgg cgtttatcag gctgggataa ggtcatcgtt taccccgacg ttaagcggtc
    15841 atttgtcagt cagctatggc aatggcgcag gggtagaatc gccgtggaat actcaggcgg
    15901 gtgtggtctg gacgttctga taacaaaaaa taaacaggct gtgatgtg
//
LOCUS       DAZFWL020000104        15889 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFWL020000104 DAZFWL020000000
VERSION     DAZFWL020000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.104"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(236..1498)
                     /locus_tag="ACHKNP_RS17710"
                     /old_locus_tag="pgaptmp_003542"
     CDS             complement(236..1498)
                     /locus_tag="ACHKNP_RS17710"
                     /old_locus_tag="pgaptmp_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4705641.1"
                     /translation="MKLNARQVETAKPKEKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPMVTLAQARAKRDEAKKLLVQGIDPKAEQKEAQAENSGAYTFET
                     IAREWHASNKRWSEDHRSRILRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFSEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMVLLEQLKQISGDKELLFPGDHDAAKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHTERNNVRAAYIHTSEHLDERRL
                     MVQWWADFLEANRNNSVTPYEFAKFMGK"
     gene            complement(1662..1746)
                     /locus_tag="ACHKNP_RS17715"
                     /old_locus_tag="pgaptmp_003543"
     tRNA            complement(1662..1746)
                     /locus_tag="ACHKNP_RS17715"
                     /old_locus_tag="pgaptmp_003543"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1710..1712),aa:Leu,seq:caa)
     gene            1942..2961
                     /gene="ahr"
                     /locus_tag="ACHKNP_RS17720"
                     /old_locus_tag="pgaptmp_003544"
     CDS             1942..2961
                     /gene="ahr"
                     /locus_tag="ACHKNP_RS17720"
                     /old_locus_tag="pgaptmp_003544"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HGU4705642.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDTC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGTDKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            3091..4593
                     /gene="yjgR"
                     /locus_tag="ACHKNP_RS17725"
                     /old_locus_tag="pgaptmp_003545"
     CDS             3091..4593
                     /gene="yjgR"
                     /locus_tag="ACHKNP_RS17725"
                     /old_locus_tag="pgaptmp_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HGU4705643.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDLMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMISELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASIEQQNNPPAKGKEIAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(4712..5794)
                     /gene="lptG"
                     /locus_tag="ACHKNP_RS17730"
                     /old_locus_tag="pgaptmp_003546"
     CDS             complement(4712..5794)
                     /gene="lptG"
                     /locus_tag="ACHKNP_RS17730"
                     /old_locus_tag="pgaptmp_003546"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU4705644.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5794..6894)
                     /gene="lptF"
                     /locus_tag="ACHKNP_RS17735"
                     /old_locus_tag="pgaptmp_003547"
     CDS             complement(5794..6894)
                     /gene="lptF"
                     /locus_tag="ACHKNP_RS17735"
                     /old_locus_tag="pgaptmp_003547"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU4705645.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMCTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6957..7007)
                     /gene="ytgA"
                     /locus_tag="ACHKNP_RS17740"
                     /old_locus_tag="pgaptmp_003548"
     CDS             complement(6957..7007)
                     /gene="ytgA"
                     /locus_tag="ACHKNP_RS17740"
                     /old_locus_tag="pgaptmp_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HGU4705646.1"
                     /translation="MSIIILATISTTQENK"
     gene            7161..8672
                     /gene="pepA"
                     /locus_tag="ACHKNP_RS17745"
                     /old_locus_tag="pgaptmp_003549"
     CDS             7161..8672
                     /gene="pepA"
                     /locus_tag="ACHKNP_RS17745"
                     /old_locus_tag="pgaptmp_003549"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HGU4705647.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9026..9469
                     /gene="holC"
                     /locus_tag="ACHKNP_RS17750"
                     /old_locus_tag="pgaptmp_003550"
     CDS             9026..9469
                     /gene="holC"
                     /locus_tag="ACHKNP_RS17750"
                     /old_locus_tag="pgaptmp_003550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HGU4705648.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTC
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9469..12324
                     /gene="valS"
                     /locus_tag="ACHKNP_RS17755"
                     /old_locus_tag="pgaptmp_003551"
     CDS             9469..12324
                     /gene="valS"
                     /locus_tag="ACHKNP_RS17755"
                     /old_locus_tag="pgaptmp_003551"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGU4705649.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12378..13574)
                     /gene="yjgN"
                     /locus_tag="ACHKNP_RS17760"
                     /old_locus_tag="pgaptmp_003552"
                     /pseudo
     CDS             complement(12378..13574)
                     /gene="yjgN"
                     /locus_tag="ACHKNP_RS17760"
                     /old_locus_tag="pgaptmp_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
     gene            13767..14270
                     /gene="yjgM"
                     /locus_tag="ACHKNP_RS17765"
                     /old_locus_tag="pgaptmp_003553"
     CDS             13767..14270
                     /gene="yjgM"
                     /locus_tag="ACHKNP_RS17765"
                     /old_locus_tag="pgaptmp_003553"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4705650.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(14316..14732)
                     /gene="rraB"
                     /locus_tag="ACHKNP_RS17770"
                     /old_locus_tag="pgaptmp_003554"
     CDS             complement(14316..14732)
                     /gene="rraB"
                     /locus_tag="ACHKNP_RS17770"
                     /old_locus_tag="pgaptmp_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HGU4705651.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14894..>15889
                     /gene="argF"
                     /locus_tag="ACHKNP_RS17775"
                     /old_locus_tag="pgaptmp_003555"
     CDS             14894..>15889
                     /gene="argF"
                     /locus_tag="ACHKNP_RS17775"
                     /old_locus_tag="pgaptmp_003555"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGU4705652.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGAHVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATL"
BASE COUNT         4066 a         4027 c         4111 g         3685 t
ORIGIN      
        1 caccgactaa aactgtaatg aacagagtta gtcggctggt ttctataaca aaagccatac
       61 cgtttgaaca ctttttgttt tttcactatc atctcagaat ggccattgca cctcgctcta
      121 aaataatgcc ccttggttga ggttttcccg cattttaaaa gagcatttca atttattatt
      181 atataattct ctcacacaat taccaaatta gttaatgtat aatatttcag ttacattatt
      241 tccccatgaa tttagcaaat tcataaggtg taacagagtt atttcgattg gcctcaagaa
      301 aatcagccca ccattgaacc attaatctac gttcgtctaa atgctcagag gtatgaatat
      361 atgctgcacg cacattgtta cgctctgtat gactcaactg ccgttctatc gcgtcatcgc
      421 tccataagcc tgattcacca agcgcgccac gcgccatcgt cctaaatcca tgcccacaga
      481 cctcagtttt cgtgtcatac cccatcgcac gcaaggcgct atttactgtg ttctcactca
      541 taactttggc cgcgtcgtga tcccctggaa aaagtaactc tttatcgcca ctaatctgct
      601 tgagttgctc taacaaaacc atcgcctgtc gactaagagg aacgatatgc tcctctttca
      661 tcttcatgcc acgatacgag taacgcacgc ctttaatttc ttctcgcttt gcaggtatac
      721 gccaaagaga tttatcgaag tcgaactcat cccatcgcgc gaaacgtaac tcactggaac
      781 gtacaaaagt tagtaaggaa agctcgaccg cgatccttgt cattacacga ccacgatatg
      841 cagcaagacg tgcaagaaac tcagagaagc ggctagaggg taaagctgga tagtgtcgtg
      901 ctttggttgt agataacgca ccggccatgt cactggctgg attagagtcg atgtaatcgt
      961 tctgtacggc ataacgcata atggccgtga cgcgctgttg caggcgctga gcgacatcgt
     1021 gtttgccact ggcatcaact tttttaatcg gggctaacag gtggctggtt ttaagctgcc
     1081 gaatgtcgga cgaaccgata tgaggaaaaa tataaagctc aagatagcgc agaatgcgtg
     1141 atcgatggtc ttcactccag cgcttattac tggcatgcca ttcacgagca attgtttcga
     1201 aagtgtatgc gcccgaattc tcagcctgag cttctttctg ctcggctttt gggtcaatgc
     1261 cctgcactaa gagcttttta gcctcgtcac gctttgctct tgcctgagca agtgtcacca
     1321 tgggccaaac accaaaagcg aggcgatcct cttttttgtc agaggggcgt ctgtatttca
     1381 tgcgccagta tttagaaccc ttagccgaaa cctcgagata caaaccgccg ccatcggcca
     1441 ttttgtaggt tttctctttt ggctttgctg tctcgacctg tctggcgttg agcttcattt
     1501 gggggcacat ttctaatcga agttaagatg cccccaatta tgcccccaat agcatccgga
     1561 tttcaacgga ctagctcgga cagctcagga cagaaaaacc gctacaggca ttgattttaa
     1621 agggtttgct ggattttctc ggatggtctt ggaagtacta atggtgccga aggccggact
     1681 cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg atttcgccac
     1741 ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca cgccaccatg
     1801 caagcgccag cttgcaccca cctcgctaag tgctgaaatt ttcagcatta cactcagcga
     1861 tatgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac ttcccaaaca
     1921 acaccagaga aggacccaaa aatgtcgatg ataaaaagct atgccgcaaa agaagcgggc
     1981 ggcgaactgg aagtttatga gtacgatccc ggtgagctga agccacaaga tgttgaagtg
     2041 caggtggatt actgcgggat ttgtcattcc gatctgtcga tgatcgataa cgaatgggga
     2101 ttttcacaat atccgctggt tgccgggcat gaggtgattg ggcgcgtggt ggcactcggg
     2161 agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg ctggacggcg
     2221 cgtagctgtg gtcactgcga cacctgtatt agcggtaatc agatcaactg cgagcaaggt
     2281 gcggtgccga cgattatgaa tcgcggtggc tttgccgaga agttgcgtgc ggactggcaa
     2341 tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct gttgtgcggc
     2401 ggtatcacgg tctttaaacc actgttgatg caccatatca ctgctaccag ccgcgttggg
     2461 gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc aatgggatgc
     2521 gaggtgacgg cctttagttc taatccggcg aaagagcagg aagtactggc gatgggtacc
     2581 gataaagtgg tgaatagccg cgatccgcag gcactgaaag ccctgtcggg gcagtttgat
     2641 ctcattatca atactgtgaa cgtcagcctc gactggcagc cttattttga ggcgctgacc
     2701 tatggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt tccggccttt
     2761 acgttaattg cgggcgaccg cagcatctct ggttctgcta ccggcacgcc ttatgagctg
     2821 cgtaagctga tgcgttttgc cgcccgcagc aaggttgcgc cgaccaccga actgttcccg
     2881 atgtcgaaaa ttaacgacgc catccagcat gtacgcgatg gtaaagcgcg ctaccgcgtg
     2941 gtcctgaaag ccgacttctg atccctttgc cgggagcctg tcgcccggca cttaaatctc
     3001 tttggaaggc gtctcgcaat ctgctgaatt tagcggtgaa gagtgcacag ccgttgccta
     3061 tactccaggc aggaaactgg aggaaatctc atgagcgaac ccctgttaat tgcccgcacg
     3121 ccggacacag aactgttttt actgccggga atggctaacc gtcacgggct gattactggc
     3181 gcaacgggga cgggtaaaac tgtcacgctg caaaaactgg cggagtctct atcggaaatc
     3241 ggcgtgccgg tgtttatggc cgatgtgaaa ggcgatctga ccggtatcgc gcaggcagga
     3301 acggcgtcgg aaaaactgct cgcaaggctt aaaaatatcg gcgtaaatga ctggcaaccg
     3361 catgctaatc cggtggtggt gtgggatatc tttggcgaga aaggccatcc ggtgcgggcg
     3421 acggtttcgg atctggggcc gctgttgctg gcgcggctgt tgaatctcaa cgatgtgcaa
     3481 tctggcgtgc tgaatatcat cttccgaatt gctgacgatc aggggctgtt actgctcgac
     3541 tttaaagatc tgcgggcgat tacccagtac atcggcgata acgccaaatc tttccagaat
     3601 cagtacggaa atatcagtag cgcatcggtt ggtgccatcc agcgcgggct gttgtcgctg
     3661 gaacagcaag gcgcagcaca cttctttggt gagccgatgc tggatatcaa agacttgatg
     3721 cgcaccgatg ccaacggtaa aggcgttatt aatatcctca gcgccgagaa gctttatcag
     3781 atgccgaaac tgtacgccgc cagcctgctg tggatgattt cagagttgta tgaacaattg
     3841 ccggaagcgg gcgatctgga gaagccaaaa ctggtgtttt tcttcgacga agcacatctg
     3901 ctgtttaacg atgcaccgca ggtactgctg gataagattg agcaggtgat aaggcttatt
     3961 cgctcaaaag gcgtgggcgt ctggttcgtt tcgcaaaacc cgtctgatat tccggataat
     4021 gtgctcggcc agctcggtaa tcgcgttcag cacgctttgc gggcttttac gccaaaagat
     4081 cagaaagcgg taaaagctgc ggcgcaaacc atgcgggcca atccggcgtt tgataccgaa
     4141 aaggcaatcc aggaactggg gaccggcgag gcgttaatct cgtttctcga tgcaaaagga
     4201 agtccttctg tggtggaacg ggcgatggtg attgcgcctt gttcgcggat gggaccggtg
     4261 acggaagatg agcgcaatgg cttgattaac cactccccgg tgtatggcaa gtacgaggat
     4321 gacgtggacc gggaatccgc ctatgagatg ttgcaaaaag gcgtacaggc gagtattgaa
     4381 cagcaaaata atcctccagc gaaagggaaa gagatagcgg tagatgacgg cattcttggt
     4441 ggattgaagg atattttgtt cggcactacc ggaccacgcg gcgggaagaa agatggtgtg
     4501 gtgcagacaa tggccaaaag cgccgctcgc caggtgacga atcagattgt acgcgggatg
     4561 ttggggagtt tgctgggagg gagaagaagg taagtcaggc gcgatctgtc cccctcaccc
     4621 taaccctctc cccaaagggg cgaggggacc gatcgaactc gtatttgact ttatcaacaa
     4681 tgtgaaaggg agctttcgct cccttgcttg gttacgattt tctcattaac agccacaggc
     4741 tgattaagaa gaagctggcg cttggcaaca gtgcgccgat aatcggcggg atgccgtaaa
     4801 ccaacgtcag cgggccgaag atctggtcca gtacgtagaa gacaaaaccg aagctgatac
     4861 cggtgaccac acgcacgccc atcggtacgc tacgcagtgg gccaaagatg aacgacagcg
     4921 ccatcagcat catcaccgcc acggatagcg gctggaagat tttgctccac atattgagct
     4981 gataacgtcc ggcatcctga ccgctcgact tcagatactt cacgtagttg tgcaaaccgc
     5041 tgattgagag tgcatccggg tccagcgcca ccacgcccag tttgtctggc gtgaggttgg
     5101 ttttccaggt gccgctcacc gtctgcgaac cggtgatctg tttcggattg gtcagatcag
     5161 attcatcaac ctgcgacaga cgccagactt tatgttccgg gtcaaacttc gcagtagcgg
     5221 catagcgtac ggattgcaga cgacgatttt cgttaaaggc ataaatgctg atgccaccta
     5281 actcttcgtc acctttaacc cgctcaatgt agacgaagtt gttgccatct ttcgcccata
     5341 agccttgctg ggtagagagc aacgagccgc cgtacatcgc ctgcgcacgg tagttacgcg
     5401 ccatctgctc gccctgcggc gcgacccatt cgccaatcgc catcgtcagc aagaccagcg
     5461 gaatggcggt tttcatcacc gacagcgcca cctgcatacg ggtaaaacca gaagcctgca
     5521 tcaccaccag ttcgctgcgc tgcgccagca tcccaagacc aagcaacgcc ccaagcagag
     5581 ccgccatcgg gaagaagatt tgcacatctt tcggcacgct cagcaaggta tacattcctg
     5641 cgcctaacgc gtcgtaactc ccctgcccgg cttttttcaa ctgatcgaca aatttgataa
     5701 tgcccgacag cgacaccagc atgaacagcg tcatcatgat ggtggtgaaa atagttttac
     5761 cgatatagcg gtcaagtacg ccaaaaggtt gcatcacacc gctcctttac gcgaaaaact
     5821 ggcgcgcagg cggcggaccg gcacggtgtc ccaaaggttg agaacaatcg ccaaagccag
     5881 ataaatcagg ttaacggtcc acatccacag cgtcgggtcc agcttacctt taccgccgtt
     5941 cgatttcagg gaggtctgga tcaggaagaa aagtagatac agcagcatgg ctggcagcat
     6001 cgacagtacg cgtccctgac gtgggttaac cacgctcagc ggtacgacca taagtgccat
     6061 cataaacacg gtgaatacca acgtgatacg ccagttcagt tctgcgcgag cacgatcggt
     6121 gtcagtgttc cacaatgtgc acatgtccat ctggtcggta tcgttcgggt cgagcgccac
     6181 cgcctggtga ccaatgatcg cctgataatc ctggaaatcc gtaatgcgga aatcacgtaa
     6241 cagtgcagtg ccttcgaagc gcgttccctg gttgagagtg acgacctggg agccgtcgcg
     6301 cagctgggtt aaatgtccgg aatcggccac caccacagaa ggacgtgcat taccttttgg
     6361 tcgaatttgt gcgaggaaca catctttgaa atcgctgccg tcaacgcttt cgatgaacag
     6421 caccgagctg ccattagtcg cttgctggaa ttgcccttgc gccagcgccg ccatgccagg
     6481 gttcgctttc gcttctgcta acacttcatc ctgatgacgc gatgaccacg gtcccgccca
     6541 catcacgtta accgccgcga cgattgccgt gaataccgca aggatcattg ccgctttcac
     6601 cagaaccgct ttgctcaggc cgcaggcatg cattaccgta atttcacttt cggtatacag
     6661 tttgcccagc gtcatcagca gcccgaggaa caggcttaat ggcaggataa gctgcgccat
     6721 ttccggcacg cccaacccga gaagggagag caccagattc gccggaatat cgccgtcaac
     6781 cgctgcgccg aggatcctca ctaacttttg acagaagaag atcaaaagca agatgaagag
     6841 tatcgccagc tggcttttga gcgtctcccg caccagatat cttatgatta tcactttaaa
     6901 tacgcccgta aaaactcgtc ttttgcagga ttttagcttg tttcatggct taaacgtcat
     6961 ttattctctt gagtcgtcga aatcgtcgct aagataatta tactcaacgg attcacctct
     7021 cagattttgt tctgacgtgc caatgccgta ataacgttaa gattaacacg aagtcatcgc
     7081 aacagcggac atgagttacg aaagcttgca attctatctg tagccaccgc cgttgtcttt
     7141 aagattcagg agcgtagtgc atggagttta gtgtaaaaag cggtagcccg gagaaacagc
     7201 ggagtgcctg catcgtcgtg ggcgtcttcg aaccacgtcg cctttctccg attgcagaac
     7261 agctcgataa aatcagcgat gggtacatca gcgccctgct acgtcggggc gaactggaag
     7321 gaaaaccggg gcagacattg ttgctgcacc atgttccgaa tgtactttcc gagcgaattc
     7381 tccttattgg ttgcggcaaa gaacgtgagc tggatgagcg tcagtacaag caggttattc
     7441 agaaaaccat taatacgctg aatgatactg gctcaatgga agcggtctgc tttctgactg
     7501 aactgcacgt taaaggccgt aacaactact ggaaagtgcg tcaggctgtc gagacggcaa
     7561 aagaaacgct ctacagtttc gatcagctga aaacgaacaa gagcgaaccg cgtcgtccgc
     7621 tgcgtaaaat ggtgttcaac gtgccgaccc gccgtgaact gaccagcggt gagcgcgcga
     7681 tccagcacgg tctggcgatt gccgccggga ttaaagcagc aaaggatctt ggcaatatgc
     7741 cgccgaatat ctgtaacgcc gcttacctcg cttcacaagc gcgccagctg gctgacagct
     7801 acagcaagaa tgttatcacc cgcgttatcg gcgaacagca gatgaaagag ctggggatgc
     7861 attcctatct ggcggtcggt cagggttcgc agaacgaatc gctgatgtcg gtgattgagt
     7921 acaaaggcaa cgcgtcggaa gatgcacgcc caatcgtgct ggtgggtaaa ggtttaacct
     7981 tcgactccgg cggtatctcg atcaagcctt cagaaggcat ggatgagatg aagtacgata
     8041 tgtgcggtgc ggcagcggtt tacggtgtga tgcgtatggt cgcggagcta caactgccga
     8101 ttaacgttat cggcgtgttg gcaggctgcg aaaacatgcc tggcggacga gcctatcgtc
     8161 cgggcgatgt gttaaccacc atgtccggtc aaaccgttga agtgctgaat actgacgctg
     8221 aaggccgcct ggtactgtgc gacgtgttaa cttacgttga gcgttttgag ccggaagcgg
     8281 tgattgacgt ggcgacgctg accggtgcct gcgtgatcgc gctgggtcat cacattaccg
     8341 gtctgatggc gaaccataat ccgctggccc atgaactgat tgccgcgtct gaacaatccg
     8401 gtgaccgcgc atggcgctta ccgctgggtg acgagtatca ggaacagctg gagtccaatt
     8461 ttgccgatat ggcgaacatt ggcggtcgtc ctggtggggc gattaccgca ggttgcttcc
     8521 tgtcgcgctt tacccgtaag tacaactggg cgcacctgga tatcgccgga accgcctggc
     8581 gttctggtaa agcaaaaggc gcaaccggtc gtccggtagc gttgctggca cagttcctgt
     8641 tgaaccgcgc tgggtttaac ggcgaagagt aattgcgtca ggcaaggctg ttattgccgg
     8701 atgcggcgtg aacgccttat ccgacctaca cagcactgaa ctcgtaggcc tgataagacg
     8761 cgcaagcgtc gcatcaggca atgctgttat tgtcggatgc ggcatgaacg ccttatccga
     8821 cctacacagc accgaactcg taggcctgat aagacacaac agcgtcgcat caggcaatgc
     8881 tgttattgtc ggatgcggcg tgagcgcctt atccgaccta cacacagcac tgaactcgta
     8941 ggcctgataa gacacaacag cgtcgcatca ggcgctgcgg tgtatacctg atgcgtattt
     9001 aaatccacca caagaagccc catttatgaa aaacgcgacg ttctaccttc tggacaatga
     9061 caccaccgtc gatggcttaa gcgccgttga gcaactggtg tgtgaaattg ccgcagaacg
     9121 ttggcgcagc ggtaagcgcg tgctcatcgc ctgtgaagat gaaaagcagg cttaccggct
     9181 ggatgaagcc ctgtgggcgc gtccggcaga aagctttgtt ccgcataatt tagcgggaga
     9241 aggaccgcgt ggcggtgcgc cggtggagat cgcctggccg caaaaacgca gcagcagccc
     9301 gcgcgatata ttgattagtc tgcgaacatg ctttgcagat tttgccaccg ctttcacaga
     9361 agtggtagac ttcgttcctt atgaagattc tctgaaacaa ctggcgcgcg aacgctataa
     9421 agcctaccgc gtggctggtt tcaacctgaa tacggcaacc tggaaataat ggaaaagaca
     9481 tataacccac aagatatcga acagccgctt tacgagcact gggaaaagca gggctacttt
     9541 aagcctaatg gcgatgaaag ccaggaaagt ttctgcatca tgatcccgcc gccgaacgtc
     9601 accggcagtt tgcatatggg tcacgccttc cagcaaacca tcatggatac catgatccgc
     9661 tatcagcgca tgcagggcaa aaacaccctg tggcaggtcg gtactgacca cgccgggatc
     9721 gctacccaga tggtcgttga gcgcaagatt gccgcagaag aaggtaaaac ccgtcacgac
     9781 tacggccgcg aagctttcat cgacaaaatc tgggaatgga aagcggaatc cggcggcacg
     9841 attacccgtc agatgcgccg tctcggcaac tccgtcgact gggagcgtga acgcttcacg
     9901 atggacgaag gcctgtccaa tgcggtgaaa gaagttttcg ttcgtctgta taaagaagac
     9961 ctgatttacc gtggcaaacg cctggtaaac tgggacccga aactgcgcac cgctatctct
    10021 gacctggaag tggaaaaccg cgaatcgaaa ggttcgatgt ggcacatccg ctatccgctg
    10081 gctgacggtg cgaaaaccgc agacggtaaa gattacctgg tggttgcgac tacccgtccg
    10141 gaaaccctgc tgggcgatac cggtgtagcc gttaacccgg aagatccgcg ttacaaagac
    10201 ctgattggca aatatgtcat tctaccgctg gttaaccgtc gtattccgat cgttggcgac
    10261 gaacacgccg acatggaaaa aggcaccggc tgcgtgaaga tcaccccggc gcacgacttt
    10321 aacgactatg aagtcggtaa acgtcacgcc ctgccgatga tcaacatcct gacctttgac
    10381 ggcgatatcc gtgaaagcgc ccaggtgttc gataccaaag gtaacgaatc tgacgtttat
    10441 tccagcgaga tcccggcaga attccagaaa ctggagcgtt ttgccgcacg taaagcagtc
    10501 gttgctgcgg ttgacgcact cggcctgctg gaagaaatta aaccgcacga cctgaccgtt
    10561 ccttacggcg accgtggcgg cgtagttatc gaaccaatgc tgaccgacca gtggtacgtg
    10621 cgtgccgatg tactggcgaa accggcggtt gaagcggttg agaacggcga cattcagttc
    10681 gtaccgaagc agtacgaaaa catgtatttc tcctggatgc gcgatattca ggactggtgt
    10741 atctctcgtc agctgtggtg gggtcaccgt atcccggcat ggtatgacga agcgggtaat
    10801 gtttatgttg gccgcaacga agaagaagtg cgcaaggaaa ataacctcgg tgccgacgtt
    10861 gccctgcgtc aggacgaaga cgttctcgac acctggttct cttctgcgct gtggaccttc
    10921 tctacccttg gctggccgga aaacaccgac gccctgcgtc agttccaccc aaccagcgtg
    10981 atggtatccg gcttcgacat catcttcttc tggattgccc gcatgatcat gatgaccatg
    11041 cacttcatca aagatgaaaa tggcaaaccg caggtgccgt tccacactgt ttacatgacc
    11101 ggtctgattc gtgacgacga aggccagaaa atgtccaaat ccaagggtaa cgttatcgac
    11161 ccgctggata tggttgacgg tatttcgctg ccagaactgc tggaaaaacg taccggcaat
    11221 atgatgcagc cgcagctggc ggacaaaatc cgtaagcgca ccgagaaaca gttcccgaac
    11281 ggcattgagc cgcacggcac cgatgccctg cgcttcaccc tggcggcgct ggcgtctacc
    11341 ggtcgtgaca tcaactggga tatgaagcgc ctggaaggtt accgtaactt ctgtaacaag
    11401 ctgtggaacg ccagccgctt tgtgctgatg aacacagaag gtcaggattg cggctttaac
    11461 ggcggcgaaa tgacactgtc gctggcggac cgctggattc tggcagagtt caaccagacc
    11521 atcaaagcgt accgcgaagc gctggacagc ttccgcttcg atatcgctgc aggcattctg
    11581 tatgagttca cctggaacca gttctgtgac tggtatctgg agctgaccaa gccggtaatg
    11641 aacggtggca ccgaagccga actgcgcggt actcgccata cgctggtgac tgtactggaa
    11701 ggtctgctgc gcctcgcgca tccgatcatt ccgttcatca ccgaaaccat ctggcagcgt
    11761 gtgaaagtac tttgcggtat cactgccgac accatcatgc tgcagccgtt cccgcagtac
    11821 gatgcatctc aggttgatga agccgcactg gccgacaccg aatggctgaa acaggcgatc
    11881 gttgcggtac gtaacatccg tgcagaaatg aacatcgcgc cgggcaaacc gctggagctg
    11941 ctgctgcgtg gttgcagcgc ggatgcagaa cgtcgcgtaa atgaaaaccg tggcttcctg
    12001 caaaccctgg cgcgtctgga aagcatcacc gtgctgcctg ccgatgacaa aggtccggtt
    12061 tccgttacga agatcatcga cggcgcagag ctgctgatcc cgatggctgg cctcatcaac
    12121 aaagaagatg agctggcgcg tctggcgaaa gaagtggcga agatcgaagg cgaaatcagc
    12181 cgtatcgaga acaagctggc gaatgaaggc tttgtcgccc gcgcaccgga agcggtcatc
    12241 gcgaaagagc gtgagaagct ggaaggctat gcggaagcga aagcgaagct gattgaacag
    12301 caggctgtta tcgccgcgct gtaatcaaca ccgtgatgaa aacgaaggcc ggagaatgct
    12361 ccggcctttt ttatctctta cagaaatggt aaagaaggca taattccccg tgaaatactg
    12421 gctaagaagc ctttatctgg ttgttcttct ttattgatta aaggaaggtc atccaaatcg
    12481 cccagcaaat acgtattttt tgcctgccag tcaatcatcc atattttcag cagtggataa
    12541 gccagaccgc ccgtaatccc ggatatcact accaacgcac acatgcgata aagcataccg
    12601 tggtaagtta aagttgagcg aaaacgaata cgcccatcat tcagtgacag gttgctcata
    12661 aaatgatttc gcaacgacac cgttaaataa ctggtgctaa cagcaatccc aaaataatag
    12721 attaactgcg cgattatcat tttacgttgc atttccataa attgctgtaa attctcaata
    12781 tcatcatttg catacacaga actatcatac gcatttaata tttgatcgaa gataatataa
    12841 ccagcaacag cgagaaaagg taataaagct aaaaatgcga ggatggcata ttttatacaa
    12901 tacgcagtat ctaatttcac cttgaaacga tgtataccga aactggtatt gctccagaga
    12961 aaactcatta ccaggctata taaagtacca ttaaaaatac caatggaaac aataccaact
    13021 attgccatca gaaccacaga tataataaca ctacttgaac tattggcatg tagcatcttt
    13081 gtcgagataa agaaaacagt ccccatccca atggccatta aaatcggcaa gaaaaaggtt
    13141 actcaccaga accctttcat agagcaatta aaactaaatc ttacgccgtt gagactagtc
    13201 atcaaggcct gataacgcag tccttttgct gccataaata taagcaaaac caacagtgac
    13261 aaagtcaaaa cacagccaat aagcggcata tctgctgaca ctgtcattaa gattgcgaaa
    13321 tagaaaaaaa caaaaacaag acaactaaca aaaacattcc caccggtaat cccataagaa
    13381 aatcgttgtc cgttaacctc catattagca tagagataac gcttacattt cattaatgcc
    13441 catggtaaat agatacctag cgttataatc gttaacaaca cattcaccac acaaataaga
    13501 aaatatctct cacctgtacc atgaaaaaca aacgaatggg acggaacatc catttcatta
    13561 ataacttgag ccataaaaat tccctgaatt aaaaaatcca ttgatataag ttatagatac
    13621 agacagtaaa atgttctgcc acgttacaat actcttatca gtaataggct ggcaaaaact
    13681 ttattgcttt ataagatgct tgcgcacgta agaagagagt ggtattaaac aaatgtataa
    13741 ttactttatt tttattgggt taaactatga ataacattgc gccgcaatca cctgtaatgc
    13801 gtcgcctgac gctgcaggat aatcctgcta ttgcccgcgt cattcgtcag gtatccgccg
    13861 aatacggtct taccgctgat aaaggctaca ccgtcgccga tccgaatctt gacgagctgt
    13921 atcaagtgta tagtcagccg ggtcatgcat attgggtggt tgagtacgag ggtgaagtgg
    13981 tcggcggcgg tgggattgcg ccattagcag gtagtgaatc ggacatttgc gaactgcaaa
    14041 agatgtattt tcttccggct attcgcggta aagggctggc gaaaaaactg gccttaatgg
    14101 cgatggagca ggcacgagag atgggcttca aacgctgcta tctggaaacg accgcttttt
    14161 taaaggaagc cattgcgctt tatgagcatt tgggctttga gcatatcgac tatgcgcttg
    14221 gctgcacggg ccatgtcgat tgtgaagtgc ggatgctacg cgaactctaa aaaaatgccc
    14281 gctagtgtgt gctatcgggc attttaatca attaattagt ggcgaacccc gtcatcgtct
    14341 tcatcgacaa aatcttcatc gtcgccgtct tcgccgttgg gatcttcaaa gtaagtgccc
    14401 caaccgtcgt actcaacgtc aaatttctcc gccagcgtca tcagttgttc aacctgggca
    14461 tcgatcagat cggcattcaa cgcgcactcg ctgaggatgt cgcagcaaat cacgatatca
    14521 ccatcttcaa cttccagctc ttctggatcg gtcacttcgt aaccgagttt aaacgcttca
    14581 actgctgctt tttccagggt ttccagatcg tctgcggaaa gatggtgttc gatggtatac
    14641 agcgcgtccg gatcgctgcc atcttccagt aattcttcaa taatcaaacg tgtttcttca
    14701 cgctgttctt ccagttgttc cgggtttgcc atggctcatt cctcttaatg tcctgccgat
    14761 actcttattg tcacacacca ctgacattgc ctccaccttt gtgacaaaga tttatgcttt
    14821 agacttgcaa atgaataatc atccatataa attgaatttt aattcattga ggtgttagcc
    14881 acaggaggga tctatgtccg ggttttatca taagcatttc ctgaaattac tcgatttcac
    14941 gccagctgaa ctcaacagcc tgctgcagtt agccgcgaag ctgaaagccg ataagaaaag
    15001 cggcaaagaa gaagccaaac tcactggtaa aaacatcgcg ctcatcttcg aaaaagactc
    15061 gacccgtacc cgatgctctt tcgaagttgc cgcatatgac cagggtgctc acgttactta
    15121 tctcggccca agcggcagtc agattggtca taaagagtcg attaaagaca ctgcccgcgt
    15181 gctgggtcgc atgtatgacg gtattcagta tcgcggctat ggtcaggaga ttgtcgaaac
    15241 actggcggaa tacgctggcg tgccggtatg gaacggcctg accaatgagt ttcaccccac
    15301 gcagttgctg gcggatctcc tcaccatgca ggagcattta cccggtaaag cattcaacga
    15361 aatgacgctg gtctatgcag gtgacgcacg taacaacatg ggcaattcga tgctcgaagc
    15421 tgcggcgctt accggtctgg atttgcgtct ggtcgcgcca caggcatgct ggccggaagc
    15481 tgcgctggtt acggaatgcc gcgctctggc acagcaaaat ggcgggaata ttacgctgac
    15541 ggaagatgta gctaaaggag ttgaaggtgc tgactttatc tataccgatg tatgggtgtc
    15601 gatgggtgaa gcaaaagaga aatgggctga acgcattgca ttgctgcgtg attatcaagt
    15661 gaacagcaag atgatgcagt tgaccggtaa cccggaggtc aaattcctcc actgcctgcc
    15721 cgcgtttcat gacgaccaaa cgacgcttgg caagaaaatg gcggaagagt ttggcctaca
    15781 tggcggaatg gaagtgactg atgaggtctt cgaatctgcc gccagcattg tatttgatca
    15841 ggcggaaaac cgcatgcata ctatcaaagc ggtgatggtc gcgacgctc
//
LOCUS       DAZFWL020000105        15751 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFWL020000105 DAZFWL020000000
VERSION     DAZFWL020000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.105"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            275..679
                     /gene="sdhC"
                     /locus_tag="ACHKNP_RS17780"
                     /old_locus_tag="pgaptmp_003556"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             275..679
                     /gene="sdhC"
                     /locus_tag="ACHKNP_RS17780"
                     /old_locus_tag="pgaptmp_003556"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU4705653.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            673..1020
                     /gene="sdhD"
                     /locus_tag="ACHKNP_RS17785"
                     /old_locus_tag="pgaptmp_003557"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             673..1020
                     /gene="sdhD"
                     /locus_tag="ACHKNP_RS17785"
                     /old_locus_tag="pgaptmp_003557"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HGU4705654.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1020..2786
                     /gene="sdhA"
                     /locus_tag="ACHKNP_RS17790"
                     /old_locus_tag="pgaptmp_003558"
     CDS             1020..2786
                     /gene="sdhA"
                     /locus_tag="ACHKNP_RS17790"
                     /old_locus_tag="pgaptmp_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HGU4705655.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEVSLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2802..3518
                     /gene="sdhB"
                     /locus_tag="ACHKNP_RS17795"
                     /old_locus_tag="pgaptmp_003559"
     CDS             2802..3518
                     /gene="sdhB"
                     /locus_tag="ACHKNP_RS17795"
                     /old_locus_tag="pgaptmp_003559"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HGU4705656.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYDKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3819..6620
                     /gene="sucA"
                     /locus_tag="ACHKNP_RS17800"
                     /old_locus_tag="pgaptmp_003560"
     CDS             3819..6620
                     /gene="sucA"
                     /locus_tag="ACHKNP_RS17800"
                     /old_locus_tag="pgaptmp_003560"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HGU4705657.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6635..7852
                     /gene="odhB"
                     /locus_tag="ACHKNP_RS17805"
                     /old_locus_tag="pgaptmp_003561"
     CDS             6635..7852
                     /gene="odhB"
                     /locus_tag="ACHKNP_RS17805"
                     /old_locus_tag="pgaptmp_003561"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HGU4705658.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7946..9112
                     /gene="sucC"
                     /locus_tag="ACHKNP_RS17810"
                     /old_locus_tag="pgaptmp_003562"
     CDS             7946..9112
                     /gene="sucC"
                     /locus_tag="ACHKNP_RS17810"
                     /old_locus_tag="pgaptmp_003562"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HGU4705659.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9112..9981
                     /gene="sucD"
                     /locus_tag="ACHKNP_RS17815"
                     /old_locus_tag="pgaptmp_003563"
     CDS             9112..9981
                     /gene="sucD"
                     /locus_tag="ACHKNP_RS17815"
                     /old_locus_tag="pgaptmp_003563"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HGU4705660.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10085..10807)
                     /gene="mngR"
                     /locus_tag="ACHKNP_RS17820"
                     /old_locus_tag="pgaptmp_003564"
     CDS             complement(10085..10807)
                     /gene="mngR"
                     /locus_tag="ACHKNP_RS17820"
                     /old_locus_tag="pgaptmp_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HGU4705661.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            10976..12892
                     /gene="mngA"
                     /locus_tag="ACHKNP_RS17825"
                     /old_locus_tag="pgaptmp_003565"
     CDS             10976..12892
                     /gene="mngA"
                     /locus_tag="ACHKNP_RS17825"
                     /old_locus_tag="pgaptmp_003565"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HGU4705662.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLGSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            12910..15543
                     /gene="mngB"
                     /locus_tag="ACHKNP_RS17830"
                     /old_locus_tag="pgaptmp_003566"
     CDS             12910..15543
                     /gene="mngB"
                     /locus_tag="ACHKNP_RS17830"
                     /old_locus_tag="pgaptmp_003566"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HGU4705663.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YEMAVPRNLQERSARQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            15568..>15751
                     /locus_tag="ACHKNP_RS17835"
                     /old_locus_tag="pgaptmp_003567"
     CDS             15568..>15751
                     /locus_tag="ACHKNP_RS17835"
                     /old_locus_tag="pgaptmp_003567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705664.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGF"
BASE COUNT         3764 a         3966 c         4348 g         3673 t
ORIGIN      
        1 ggataaaacc cgacaaacta tatgtaggtt aattgtaatg attttgtgaa cagcctatac
       61 tgccgccagg tctccggaac accctgcaat cccgagccac ccagcgttgt aacgtgtcgt
      121 tttcgcatct ggaagcagtg ttttgcatga cgcgcagtta tagaaaggac gctgtctgac
      181 ccgcaagcag accggaggaa ggaaatcccg acgtctccag gtaacagaaa gttaacctct
      241 gtgcccgtag tccccaggga ataataagaa cagcatgtgg gcgttattca tgataagaaa
      301 tgtgaaaaaa caaagacctg ttaatctgga cctacagacc atccggttcc ccatcacggc
      361 gatagcgtcc attctccatc gcgtttccgg tgtgatcacc tttattgcag tgggtatcct
      421 gctgtggctt ctgggtacca gcctctcttc ccctgaaggt ttcgagcaag cttccgcgat
      481 tatgggcagc ttcttcgtca aatttatcat gtggggcatc cttaccgctc tggcgtatca
      541 cgtcgtcgta ggtattcgcc acatgatgat ggattttggc tatctggaag aaacattcga
      601 agcgggtaaa cgctccgcca aaatctcctt tgttattact gtcgtgcttt cacttctcgc
      661 aggagtcctc gtatggtaag caacgcctcc gcattaggac gcaatggcgt acatgatttc
      721 atcctcgttc gcgctaccgc tatcgtcctg acgctctaca tcatttatat ggtcggtttt
      781 ttcgccacca gtggcgaact gacatatgaa gtctggatcg gtttcttcgc ctctgcgttc
      841 accaaagtgt tcaccctgct ggcgctgttt tctatcttga ttcatgcctg gatcggcatg
      901 tggcaggtgt tgaccgacta cgttaaaccg ctggcattgc gcctgatgct gcaactggtg
      961 attgtcgttg cactggtggt ttacgtgatt tatggattcg ttgtggtgtg gggtgtgtga
     1021 tgaaattgcc agtcagagaa tttgatgcag ttgtgattgg tgccggtggc gcaggtatgc
     1081 gcgcggcgct gcaaatttcc cagagcggcc agacctgtgc gctgctctct aaagtcttcc
     1141 cgacccgttc ccataccgtt tctgcgcaag gcggcattac cgttgcgctg ggtaataccc
     1201 atgaagataa ctgggaatgg catatgtacg acaccgtaaa agggtcggac tatatcggtg
     1261 accaggacgc gattgaatat atgtgtaaaa ccgggccgga agcgattctg gaactcgaac
     1321 acatgggcct gccgttctcg cgtcttgatg atggtcgtat ctatcaacgt ccgtttggcg
     1381 gtcagtcgaa aaacttcggc ggcgagcagg cggcacgcac tgcggcggcg gctgaccgta
     1441 ccggtcacgc actgttgcac acgctttatc agcagaacct gaaaaaccac accaccattt
     1501 tctccgagtg gtatgcgctg gatctggtga aaaaccagga tggcgcggtg gtgggttgta
     1561 ccgcactgtg catcgaaacc ggtgaagtgg tttatttcaa agcccgcgct accgtgctgg
     1621 cgactggcgg ggcagggcgt atttatcagt ccaccactaa cgcccacatt aacactggcg
     1681 acggtgtcgg catggctatc cgcgcaggcg taccggtgca ggatatggaa atgtggcaat
     1741 tccacccgac cggcattgcc ggtgcgggcg tactggtcac cgaaggttgc cgtggtgaag
     1801 gcggttatct gctgaacaaa catggcgaac gctttatgga gcgttatgcg ccgaacgcca
     1861 aagacctggc gggccgtgat gtggtggcgc gttccatcat gattgaaatc cgtgaaggtc
     1921 gcggctgtga tggtccgtgg gggccacacg cgaaactgaa actcgatcac ctgggtaaag
     1981 aagttctcga atcccgtctg ccgggtatcc tggagctttc ccgtaccttc gctcacgtcg
     2041 atccggtgaa agagccgatt ccggttatcc caacctgtca ctacatgatg ggcggtattc
     2101 cgaccaaagt taccggtcag gcgctgactg tgaatgagaa aggcgaagat gtggttgttc
     2161 cgggactgtt tgccgttggt gaaatcgctt gtgtatcggt acacggcgct aaccgtctgg
     2221 gcggcaactc gctgctggac ctggtggtct ttggtcgcgc ggcaggtctg catctgcaag
     2281 agtctatcgc cgagcagggc gcactgcgcg atgccagcga gtctgatgtt gaagtgtctc
     2341 tggatcgcct gaaccgctgg aacaataatc gtaacggtga agatccggtg gcgattcgta
     2401 aagcgctgca agaatgtatg cagcataact tctcggtctt ccgtgaaggt gatgcgatgg
     2461 cgaaagggct tgagcagttg aaagtgatcc gcgagcgtct gaaaaatgcc cgtctggatg
     2521 acacttccag cgagttcaac acccagcgcg ttgagtgcct ggaactggat aacctgatgg
     2581 aaacggcgta tgcaacggct gtttctgcca acttccgtac cgaaagccgt ggcgcacata
     2641 gccgcttcga cttcccggat cgtgatgatg aaaactggct gtgccactcc ctgtatctgc
     2701 cagagtcgga atccatgacg cgccgaagcg tcaacatgga accgaaactg cgcccggcat
     2761 tcccgccgaa gattcgtact tactaatgcg gagacaggaa aatgagactc gagttttcaa
     2821 tttatcgcta taacccggat gttgatgatg ctccgcgtat gcaggattac accctggaag
     2881 cggaagaagg tcgcgacatg atgctgctgg atgcgcttat ccagctgaaa gagaaagatc
     2941 ccagcctgtc gttccgccgc tcctgccgtg aaggtgtgtg cggttccgac ggtctgaaca
     3001 tgaacggcaa gaatggtctg gcctgtatta ccccgatttc ggcactcaac cagccgggca
     3061 agaagattgt gattcgcccg ctgccaggtt taccggtgat ccgcgatttg gtggtagaca
     3121 tgggacaatt ctatgcgcaa tatgacaaaa ttaagcctta cctgttgaat aatggacaaa
     3181 atccgccagc tcgcgagcat ttacagatgc cagagcagcg cgaaaaactc gacgggctgt
     3241 atgaatgtat tctctgcgca tgttgttcaa cctcttgtcc gtctttctgg tggaatcccg
     3301 ataagtttat cggcccggca ggcttgttag cggcatatcg tttcctgatc gatagccgtg
     3361 ataccgagac tgacagccgc ctcgacggtt tgagcgatgc attcagcgta ttccgctgtc
     3421 acagcatcat gaactgcgtc agtgtatgtc cgaaggggct gaacccgacg cgcgccatcg
     3481 gccatattaa gtcgatgttg ttgcaacgta atgcgtaaac cgtaggcctg ataagacgcg
     3541 caagcgtcgc atcaggcaac cagtgccgga tgcggcgtga acgccttatc cggcctacaa
     3601 gtcattaccc gtaggcctga taagcgcagc gcatcaggcg taacaaagaa atgcaggaaa
     3661 tctttaaaaa ctgcccctga cactaagaca gtttttaaag gttccttcgc gagccactac
     3721 atagacaaga gctcgcaagt gaaccccggc acgcacatca ctgtgcgtgg tagtatccac
     3781 ggcgaagtaa gcataaaaaa gatgcttaag ggatcacgat gcagaacagc gctttgaaag
     3841 cctggttgga ctcttcttac ctctctggcg caaaccagag ctggatagaa cagctctatg
     3901 aagacttctt aaccgatcct gactcggttg acgctaactg gcgttcgacg ttccagcagt
     3961 tacctggtac gggagtcaaa ccggatcaat tccactctca aacgcgtgaa tatttccgcc
     4021 gcctggcgaa agacgcttca cgttactctt caacgatctc cgaccctgac accaatgtga
     4081 agcaggttaa agtcctgcag ctcattaacg cataccgctt ccgtggtcac cagcatgcga
     4141 atctcgatcc gctgggactg tggcagcaag ataaagtggc cgatctggat ccgtctttcc
     4201 acgatctgac cgaagcagac ttccaggaga ccttcaacgt cggttcattt gccagcggca
     4261 aagaaaccat gaaactcggc gagctgctgg aagccctcaa gcaaacctac tgtggcccga
     4321 ttggtgccga gtatatgcac attaccagca ccgaagaaaa acgctggatc caacagcgta
     4381 tcgagtctgg tcgcgcgact ttcaatagcg aagagaaaaa acgcttctta agcgaactga
     4441 ccgccgctga aggccttgaa cgttacctcg gcgcaaaatt ccctggcgca aaacgcttct
     4501 cgctggaagg cggtgacgcg ttaatcccga tgcttaaaga gatgatccgc cacgctggca
     4561 acagcggcac ccgcgaagtg gttcttggga tggcgcaccg tggtcgtctg aacgtgctgg
     4621 tgaacgtgct gggtaaaaaa ccgcaagact tgttcgacga gtttgccggt aaacataaag
     4681 aacacctcgg cacgggtgac gtgaaatacc acatgggctt ctcgtctgac ttccagaccg
     4741 atggcggcct ggtgcacctg gcgctggcgt ttaacccgtc tcaccttgag attgtaagcc
     4801 cggtagttat cggttctgtt cgtgcccgtc tggacagact tgatgagccg agcagcaaca
     4861 aagtgctgcc aatcaccatc cacggtgacg ccgcagtgac cgggcagggc gtggttcagg
     4921 aaactctgaa catgtcgaaa gcgcgtggtt atgaagttgg cggtacggta cgtatcgtta
     4981 tcaacaacca ggttggtttc accacctcta atccgctgga tgctcgttct acaccgtact
     5041 gtactgatat cggtaagatg gttcaggcac cgattttcca cgttaacgcg gatgatccgg
     5101 aagccgttgc ctttgtgacc cgtctggcgc tcgatttccg taacaccttt aaacgtgatg
     5161 tcttcatcga cctggtgtgc taccgccgtc acggccacaa cgaagccgac gagccgagcg
     5221 caacccagcc gctgatgtat cagaaaatca aaaaacatcc gacaccgcgc aaaatctacg
     5281 ctgacaagct ggagcaggaa aaagtggcga cgctggaaga tgccaccgag atggttaacc
     5341 tgtaccgcga tgcgctggat gctggcgatt gcgtagtggc agagtggcgt ccgatgaaca
     5401 tgcactcttt cacctggtcg ccgtacctca accacgaatg ggacgaagag tacccgaaca
     5461 aagttgagat gaagcgcctg caggagctgg cgaaacgcat cagcacggtg ccggaagcag
     5521 ttgaaatgca gtctcgcgtt gccaagattt atggcgatcg ccaggcgatg gctgccggtg
     5581 agaaactgtt cgactggggc ggtgcggaaa acctcgctta cgccacgctg gttgacgaag
     5641 gcattccggt tcgcctgtcg ggtgaagact ccggtcgcgg taccttcttc caccgccacg
     5701 cggtgatcca caaccagtct aacggttcca cttacacgcc gttgcaacac atccataacg
     5761 ggcagggcgc gttccgcgtc tgggactccg tactgtctga agaagcagtg ctggcgtttg
     5821 aatacggtta tgccaccgca gaaccacgca ctctgaccat ctgggaagcg cagttcggtg
     5881 acttcgccaa cggtgcgcag gtggttatcg accagttcat ctcttctggc gaacagaaat
     5941 ggggccggat gtgtggtctg gtgatgttgc tgccgcacgg ttacgaaggg caggggccgg
     6001 agcactcctc cgcgcgtctg gaacgttatc tgcaactttg tgctgagcaa aacatgcagg
     6061 tgtgcgtacc gtctaccccg gcacaggttt accacatgct gcgtcgtcag gcgctgcgcg
     6121 ggatgcgtcg tccgctggtc gtgatgtcgc cgaaatccct gctgcgtcat ccgctggcgg
     6181 tttccagcct cgaagaactg gcgaacggca ccttcctgcc agccatcggt gaaatcgacg
     6241 agcttgatcc gaagggcgtg aagcgcgtag tgatgtgttc tggtaaggtt tattacgacc
     6301 tgctggaaca gcgtcgtaag aacaatcaac acgatgtcgc cattgtgcgt atcgagcaac
     6361 tctacccgtt cccgcataaa gcgatgcagg aagtgttgca gcagtttgct cacgtcaagg
     6421 attttgtctg gtgccaggaa gagccgctca accagggcgc atggtactgc agccagcatc
     6481 atttccgtga agtgattccg tttggggctt ctctgcgtta tgcaggccgc ccggcctccg
     6541 cctctccggc ggtagggtat atgtccgttc accagaaaca gcaacaagat ctggttaatg
     6601 acgcgctgaa cgtcgaataa ataaaggata cacaatgagt agcgtagata ttctggtccc
     6661 tgacctgcct gaatccgtag ccgatgccac cgtcgcaacc tggcataaaa aacccggcga
     6721 cgcagtcgta cgtgatgaag tgctggtaga aatcgaaact gacaaagtgg tactggaagt
     6781 accggcatca gcagacggca ttctggatgc ggttctggaa gatgaaggta caacggtaac
     6841 gtctcgtcag atccttggtc gcctgcgtga aggcaacagc gccggtaaag aaaccagcgc
     6901 caaatctgaa gagaaagcgt ccactccggc gcaacgccag caggcatctc tggaagagca
     6961 aaacaacgat gcgttaagcc cggcgatccg tcgcctgctg gctgaacaca atctcgacgc
     7021 cagcgccatt aaaggcaccg gtgtgggtgg tcgtctgact cgtgaagatg tggaaaaaca
     7081 tctggcgaaa gccccggcga aagagtctgc tccggcagcg gctgctccgg cggcgcaacc
     7141 ggctctggct gcacgtagtg aaaaacgtgt cccgatgact cgcctgcgta agcgtgtggc
     7201 agagcgtctg ctggaagcga aaaactccac cgccatgctg accacgttca acgaagtcaa
     7261 catgaagccg attatggatc tgcgtaagca gtacggtgaa gcgtttgaaa aacgccacgg
     7321 catccgtctg ggctttatgt ccttctacgt gaaagcggtg gttgaagccc tgaaacgtta
     7381 cccggaagtg aacgcgtcta tcgacggcga tgacgtggtt taccacaact atttcgacgt
     7441 cagcatggcg gtttctacgc cgcgcggcct ggtgacaccg gtactgcgtg atgtcgatac
     7501 cctcggcatg gcagacatcg agaagaaaat caaagagctg gcagtcaaag gccgtgacgg
     7561 caagctgacc gttgaagatc tgaccggtgg taacttcacc atcaccaacg gtggtgtgtt
     7621 cggttccctg atgtctacgc cgatcatcaa cccgccgcag agcgcaattc tgggtatgca
     7681 cgctatcaaa gatcgtccga tggcggtgaa tggtcaggtt gagatcctgc cgatgatgta
     7741 cctggcgctg tcctacgatc accgtctgat cgatggtcgc gaatccgtgg gcttcctggt
     7801 aacgatcaaa gagttgctgg aagatccgac gcgtctgctg ctggacgtgt agtagtttaa
     7861 gcttcacctg cactgtagac cgacatggat agggtgtcgg tctacggttt aaaagataac
     7921 gattactgaa ggatggacag aacacatgaa cttacatgaa tatcaggcaa aacaactttt
     7981 tgcccgctat ggcttaccag caccggtggg ttatgcctgt actactccgc gcgaagcaga
     8041 agaagccgct tcaaaaatcg gtgccggccc gtgggtagtg aaatgtcagg ttcacgctgg
     8101 tggccgcggt aaagcgggcg gtgtgaaagt tgtaaacagc aaagaagaca tccgtgcttt
     8161 tgcagaaaac tggctcggca agcgtctggt aacgtatcaa acagatgcca atggccaacc
     8221 ggttaaccag attctggttg aagcagcgac cgatatcgct aaagagctgt atctcggtgc
     8281 cgttgtagac cgtagttccc gtcgtgtggt ctttatggcc tccaccgaag gcggcgtgga
     8341 aatcgaaaaa gtggcggaag aaactccgca cctgatccac aaagttgcgc ttgatccgct
     8401 gactggcccg atgccgtatc agggacgcga gctggcgttc aaactgggtc tggaaggtaa
     8461 actggttcag cagttcacca aaatcttcat gggcctggcg accattttcc tggagcgcga
     8521 cctggcgttg atcgaaatca acccgctggt catcaccaaa cagggcgatc tgatttgcct
     8581 cgacggcaaa ctgggcgctg acggcaacgc actgttccgc cagcctgatc tgcgcgaaat
     8641 gcgtgaccag tcgcaggaag atccgcgtga agcacaggct gcacagtggg aactgaacta
     8701 cgttgcgctg gacggtaaca tcggttgtat ggttaacggc gcaggtctgg cgatgggtac
     8761 gatggacatc gttaaactgc acggcggcga accggctaac ttccttgacg ttggcggcgg
     8821 cgcaaccaaa gaacgtgtaa ccgaagcgtt caaaatcatc ctctctgacg acaaagtgaa
     8881 agccgttctg gttaacatct tcggcggtat cgttcgttgc gacctgatcg ctgacggtat
     8941 catcggcgcg gtagcagaag tgggtgttaa cgtaccggtc gtggtacgtc tggaaggtaa
     9001 caacgccgaa ctcggcgcga agaaactggc tgacagcggc ctgaatatta ttgcagcaaa
     9061 aggtctgacg gatgcagctc agcaggttgt tgccgcagtg gaggggaaat aatgtccatt
     9121 ttaattgata aaaacaccaa ggttatctgc cagggcttta ctggtagcca ggggactttc
     9181 cactcagaac aggccattgc atacggcact aaaatggttg gcggcgtaac cccaggtaaa
     9241 ggcggtacta cccacctcgg cctgccggtg ttcaacaccg tgcgtgaagc tgttgctgct
     9301 actggcgcta ccgcttctgt tatctacgta ccagcaccgt tctgcaaaga ctccattctg
     9361 gaagctattg atgcaggcat caaactgatt atcaccatca ctgaaggcat cccgacgctg
     9421 gatatgctga ctgtgaaagt gaaactggac gaagccggcg ttcgtatgat cggcccgaac
     9481 tgcccgggcg ttatcactcc gggtgaatgc aaaatcggta tccagccggg tcacattcac
     9541 aaaccgggta aagtgggtat cgtttcccgt tccggtacac tgacctatga agcggttaaa
     9601 cagaccacgg attacggttt cggtcagtcg acctgcgttg gtatcggcgg tgacccgatc
     9661 cctggctcta actttatcga cattctcgaa atgttcgaaa aagatccgca gaccgaagcg
     9721 atcgtaatga tcggtgagat cggcggtagc gctgaagaag aagcggcagc gtacatcaaa
     9781 gagcatgtta ccaagccagt tgtgggttac atcgctggtg tgactgcgcc gaaaggcaaa
     9841 cgtatgggcc acgcgggtgc catcattgcc ggtgggaaag ggactgcgga tgagaaattc
     9901 gctgctctgg aagccgcagg cgtgaaaacc gttcgcagcc tggcggatat cggtgaagca
     9961 ctgaaaactg ttctgaaata aatatctgta ataagaaata gccctcgccg cttccctcta
    10021 caggaatggc gaagggctgt cggtttcgac atggttggcc atcgtatgat ggcctttttt
    10081 gtgcttatcg cgatgatttt cgttgcgcta tcagggtaaa tttatagtca tcggtattaa
    10141 aagcgttgcg gctatattca aacacccgac catcaactaa atatccacgc gatacttttt
    10201 caagaatcgg ctttgtctgg ctgatattaa gcagacggct catctcttcg gttggcatca
    10261 gaggaatgat ttcctgttcg ctacgatcga taaccatttt cttcacttct tcaataaagt
    10321 gatattttga attttccatg acctgccagg tgagatccgg gaacaacgca agcggcatcc
    10381 aggtttcttc cagcgccatt ggcttttgct tgcgatagcg cacgcgcttc acatgccaca
    10441 cacgatcctg cgcggtgatt tgtagctgtt gctgaagaaa atcgtcagcc ggaatcactt
    10501 caaatatcag gacttcactg tgtgtatcga cgtgacggtc cgacagtttt tcatcaaaac
    10561 tggttaactg aaaaatatcg taattgaccc gctcttcttt gacgtaagtc ccgctgccct
    10621 gaatgctttc gaggatctgt tgctcgacta actggcgcaa agcctgacgc accgtaaccc
    10681 ggctgacgcc aaactctgtt tgtaatgccg attcggtagg taacgcatcg ccaggtttaa
    10741 gctcgccacg cgcaatttgt tcacgaatgc gatcggcaat ctgccggtat aagggcttgt
    10801 gtcccatttt tagtatctca ttaatacgaa tttagccatt atgcccgata aattcatcct
    10861 gtaaataata caaatacaat acaaataatt tcaatcaagt gaaattgatc acataatggt
    10921 attgttttat cgggcacact ggcgcgacta taaaaacgat caagtgagga tcatgatgaa
    10981 tctgacgact ctgacccacc gcgatgcgtt gtgtctgaat gcgcgcttta ccagccgtga
    11041 agaggccatc cacgcgttga ctcaacgtct tgctgctctg gggaaaattt ccagtactga
    11101 gcaatttctg gaagaagtgt atcgccgtga aagccttggc ccgaccgcct taggtgaagg
    11161 gttggctgtg ccgcatggca aaactgctgc ggtaaaagaa gcggcgtttg cggtcgccac
    11221 actcagcgag ccgcttcagt gggaaggcgt tgatggcccg gaagcggttg atttagtggt
    11281 gctgctggcg attcccccca atgaagcggg tactacgcat atgcaactgc tgacagcgct
    11341 gaccacgcgc ctggcggatg atgagattcg ggcgcgtata cagtcggcga cgacgcctga
    11401 tgagttgctc tcggcgctgg atgacaaggg aggcacgcaa ccttctgcct ctttttccaa
    11461 cgcgccaact atcgtctgcg taacggcctg tccggcgggt attgctcaca cctatatggc
    11521 tgcggaatat ctggaaaaag ccggacgcaa actcggcgta aatgtttacg ttgaaaaaca
    11581 aggcgctaac ggcattgaag ggcgtttaac ggcggatcaa ctcaatagtg caaccgcctg
    11641 tatttttgcg gctgaagtcg ccatcaagga gagtgagcgt tttaatggca ttcccgcgct
    11701 ttcagtgcct gttgccgagc cgattcgcca tgcagaagcg ttgatccaac aagcgcttac
    11761 cctcaagcgt agcgatgaga cgcgtaccgt acagcaagat acgcaaccgg tgaaaagtgt
    11821 caaaacggag ctgaaacagg cactgttgag cggaatctct tttgccgtac cgttgattgt
    11881 cgcggggggc acggtgctgg cggtcgcggt attactgtcg caaatcttcg ggctacaaga
    11941 tctgtttaat gaagaaaact cctggctgtg gatgtaccgc aagctgggcg gcgggctgct
    12001 cggaattttg atggtaccgg tgctcgcggc ctataccgcc tattctctgg cagataaacc
    12061 ggcgttagcg ccaggctttg cggctggact tgccgccaac atgatcggct ccgggtttct
    12121 cggcgcggtc gttggcggat tgatagccgg ttacttgatg cgctgggtga aaaatcactt
    12181 gcgtcttggc agtaaattca atggcttcct gactttttat ctctacccgg tgctcggtac
    12241 gttgggagcg ggcagtctga tgctgtttgt ggtgggagaa cctgtcgcat ggatcaataa
    12301 ctcgcttacc gcctggctga acggtctgtc aggaagtaac gcgctgttgc tgggtgccat
    12361 tctcggtttt atgtgttcct ttgaccttgg agggccagtg aataaagccg cttatgcatt
    12421 ctgcctgggc gcaatggcga acggcgttta cggcccgtat gccattttcg cctccgtcaa
    12481 aatggtttcg gcatttaccg taaccgcttc cacgatgctc gcaccgcgcc tgtttaaaga
    12541 gtttgaaatt gagaccggga aatccacctg gctgttaggg ctggcaggta ttaccgaagg
    12601 ggcgatcccg atggcgattg aagatccgct gcgggttatt ggttcgtttg tgctgggctc
    12661 tatggtaacg ggcgctattg tcggtgcgat gaatatcggc ctttcgacac ccggtgccgg
    12721 cattttctcg ctctttttac ttcatgataa tggcgcgggc ggtgttatgg cggcaattgg
    12781 ctggtttggt gcggcattgg tgggggctgc aatctcgact gcaattctcc tgatgtggcg
    12841 gcgtcacgcg gttaagcatg gcaactatct gactgatggc gtaatgccat aaacaaaaag
    12901 gaaacgacga tgaaagcggt atctcgcgtt cacatcaccc cgcatatgca ctgggatcgg
    12961 gagtggtatt tcaccaccga agagtcacgc attctgctgg tcaataatat ggaagagatc
    13021 ctgtgccgac tggaacagga caacgaatac aaatattacg tactcgacgg gcaaacggcg
    13081 atcctcgaag attatttcgc ggtgaaaccg gaaaacaaag accgtgtgaa gaaacaggta
    13141 gaagccggca agttgattat cggcccctgg tatacccaga ccgataccac gattgtttct
    13201 gcggaatcca tcgtccgtaa tctgatgtac ggaatgcgtg actgcctcgc gtttggcgag
    13261 ccgatgaaaa taggttattt accggattcc tttggcatgt ccgggcagct tccgcatatc
    13321 tacaatggat ttggcattac ccgcaccatg ttctggcgtg gatgttctga gcgccacggt
    13381 actgataaaa ccgagttttt gtggcaaagc agtgacggta gcgaagtgac ggcgcaagtg
    13441 ttgccgctgg gctacgccat cggtaagtac ttacctgccg acgaagacgg attacgcaag
    13501 cgcctcgaca gttattttga tgtgctggaa aaagcgtcag taaccaaaga aattttattg
    13561 ccgaatggtc atgaccagat gccattgcag caaaatatct tcgaagtgat ggataagcta
    13621 cgtgagatct accctcaacg taagtttgtg atgagccgct ttgaagaggt atttgagaag
    13681 atcgaagcgc agcgagataa tctggcaacc ctgaaagggg aatttattga tggcaaatat
    13741 atgcgcgtgc atcgcaccat cggttctacg cgtatggata tcaaaattgc ccacgcgcgt
    13801 attgaaaata agattgttaa tctgctggaa ccgctggcaa cactggcctg gacgttgggt
    13861 tttgaatacc accacggctt gctggagaaa atgtggaaag agatcttaaa aaatcatgcc
    13921 cacgacagta tcggttgttg ttgcagtgac aaagttcatc gcgaaatcgt cgcccgcttc
    13981 gaactggctg aagacatggc ggataatctg attcgtttct acatgcgcaa aattgccgac
    14041 aacatgccgc agagcgacgc cgacaaactc gtcctgttta acctgatgcc ctggccgcgt
    14101 gaagaagtga tcaacaccac tgtgcgcctg cgcgccagcc agtttaattt gcgggacgat
    14161 cgcggccagc ctgtgccgta ttttattcgc catgcccgtg agatcgatcc aggcctaatc
    14221 gatcggcaaa ttgttcatta tggcaattat gatcccttta tggagttcga catccagatc
    14281 aaccagattg tcccttctat gggctatcgc acgctttata ttgaagcgaa tcagcctggc
    14341 aacgtgattg cggcaaaaag tgacgctgaa gggattctgg aaaatgcttt ctggcagatt
    14401 gcgctcaatg aggatggttc tctgcaactg gtagataaag acagcggtgt gcgctatgac
    14461 cgggtattgc aaattgaaga aagctctgat gatggtgatg aatatgacta ttcacccgca
    14521 aaagaagagt gggtaattac cgcggcgaac gcgaaaccgc aatgcgatat tattcatgaa
    14581 gcctggcaga gcagggctgt tatccgttat gaaatggcgg tgccgcgtaa tctgcaagaa
    14641 cgcagcgcaa gacaatccac tggcagagta ggggtggaga tggttgtcac tctgagtcat
    14701 aacagcagac gtattgatgt ggatatcaac cttgataacc aggctgacga tcatcgcctt
    14761 cgcgtcctga tccctacacc ttttaacacc gacagtgttc tggcagatac gcagtttggt
    14821 tcgctaacgc gccccgtgaa cgacagtgca atgaacaact ggcagcaaga aggctggaaa
    14881 gaagcgccgg ttccggtatg gaatatgctc aactatgttg ccttacagga agggcgtaac
    14941 ggcatggctg tctttagcga agggctacgt gaatttgaag tcatcggtga agagaagaaa
    15001 acctttgcca ttacgttgct gcgtggcgtg ggcttactgg gcaaagaaga tctgctttta
    15061 aggcctgggc ggccttcggg aattaaaatg ccagtcccgg actcacaact acgtggtctg
    15121 ctttcttgtc gcctaagttt attgagttat accggtacgc caaccgccgc tggtgtagct
    15181 cagcaggcgc gagcatggct gactccagta cagtgttaca acaaaatccc atgggatgcg
    15241 atgaagctca acaaagccgg attcaacgtg ccggaaagtt atagtttgtt gaaaatgccc
    15301 ccagtgggat gcctgataag cgcacttaag aaagctgaag accgacaaga agtgatttta
    15361 cggctgttta atccggctga atcagcaacc tgtgatgcga ctgttgcttt cagtcgcgag
    15421 gtgatttctt gctcagaaac gatgatggat gaacacatta ccaccgagga aaatcaaggt
    15481 tcaaatctat cggggccttt tttacccggc cagtcacgga cgttcagtta ccggcttgcc
    15541 tgaatagcaa tcaaaccgaa gccacatatg cgcggccaga ttgttgacaa agggcgcttt
    15601 gttcatgccg gatacggcat gaacgcttta ttcggtctac aaaagcaggc aaattcaata
    15661 tattgcagag atgatgtaga cactggcaag cgtagcgcat caggtaattt tgcgtttatc
    15721 ttcactctca agccacgtat atgtggcttt a
//
LOCUS       DAZFWL020000106        15748 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFWL020000106 DAZFWL020000000
VERSION     DAZFWL020000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.106"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            117..968
                     /locus_tag="ACHKNP_RS17840"
                     /old_locus_tag="pgaptmp_003568"
     CDS             117..968
                     /locus_tag="ACHKNP_RS17840"
                     /old_locus_tag="pgaptmp_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HGU4705665.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESCFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLTQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            961..1332
                     /locus_tag="ACHKNP_RS17845"
                     /old_locus_tag="pgaptmp_003569"
     CDS             961..1332
                     /locus_tag="ACHKNP_RS17845"
                     /old_locus_tag="pgaptmp_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HGU4705666.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            1329..2081
                     /gene="fliP"
                     /locus_tag="ACHKNP_RS17850"
                     /old_locus_tag="pgaptmp_003570"
     CDS             1329..2081
                     /gene="fliP"
                     /locus_tag="ACHKNP_RS17850"
                     /old_locus_tag="pgaptmp_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGU4705667.1"
                     /translation="MKRRTQLTLGLGLLALAPVALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GHAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            2084..2356
                     /locus_tag="ACHKNP_RS17855"
                     /old_locus_tag="pgaptmp_003571"
     CDS             2084..2356
                     /locus_tag="ACHKNP_RS17855"
                     /old_locus_tag="pgaptmp_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HGU4705668.1"
                     /translation="MLTIDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            2358..3140
                     /gene="fliR"
                     /locus_tag="ACHKNP_RS17860"
                     /old_locus_tag="pgaptmp_003572"
     CDS             2358..3140
                     /gene="fliR"
                     /locus_tag="ACHKNP_RS17860"
                     /old_locus_tag="pgaptmp_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGU4705669.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALAIIITPLIPHPVPHDLISLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            3130..4269
                     /gene="flhB"
                     /locus_tag="ACHKNP_RS17865"
                     /old_locus_tag="pgaptmp_003573"
     CDS             3130..4269
                     /gene="flhB"
                     /locus_tag="ACHKNP_RS17865"
                     /old_locus_tag="pgaptmp_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HGU4705670.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPEVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIERLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFASFLGLQALPFKLAMNEGLSLYACVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            4253..6346
                     /gene="flhA"
                     /locus_tag="ACHKNP_RS17870"
                     /old_locus_tag="pgaptmp_003574"
     CDS             4253..6346
                     /gene="flhA"
                     /locus_tag="ACHKNP_RS17870"
                     /old_locus_tag="pgaptmp_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HGU4705671.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGADAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLASMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            6451..6729
                     /locus_tag="ACHKNP_RS17875"
                     /old_locus_tag="pgaptmp_003575"
     CDS             6451..6729
                     /locus_tag="ACHKNP_RS17875"
                     /old_locus_tag="pgaptmp_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGU4705672.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            6722..7078
                     /locus_tag="ACHKNP_RS17880"
                     /old_locus_tag="pgaptmp_003576"
     CDS             6722..7078
                     /locus_tag="ACHKNP_RS17880"
                     /old_locus_tag="pgaptmp_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGU4705673.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(7135..7908)
                     /gene="yafL"
                     /locus_tag="ACHKNP_RS17885"
                     /old_locus_tag="pgaptmp_003577"
     CDS             complement(7135..7908)
                     /gene="yafL"
                     /locus_tag="ACHKNP_RS17885"
                     /old_locus_tag="pgaptmp_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4705674.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSQLAEPFWQDHFLGARRILTEETIL"
     gene            8094..8354
                     /gene="dinJ"
                     /locus_tag="ACHKNP_RS17890"
                     /old_locus_tag="pgaptmp_003578"
     CDS             8094..8354
                     /gene="dinJ"
                     /locus_tag="ACHKNP_RS17890"
                     /old_locus_tag="pgaptmp_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HGU4705675.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            8342..8629
                     /gene="yafQ"
                     /locus_tag="ACHKNP_RS17895"
                     /old_locus_tag="pgaptmp_003579"
     CDS             8342..8629
                     /gene="yafQ"
                     /locus_tag="ACHKNP_RS17895"
                     /old_locus_tag="pgaptmp_003579"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HGU4705676.1"
                     /translation="MRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNAL
                     PLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            8785..9525
                     /gene="dpaA"
                     /locus_tag="ACHKNP_RS17900"
                     /old_locus_tag="pgaptmp_003580"
     CDS             8785..9525
                     /gene="dpaA"
                     /locus_tag="ACHKNP_RS17900"
                     /old_locus_tag="pgaptmp_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HGU4705677.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(9496..10263)
                     /gene="yafJ"
                     /locus_tag="ACHKNP_RS17905"
                     /old_locus_tag="pgaptmp_003581"
     CDS             complement(9496..10263)
                     /gene="yafJ"
                     /locus_tag="ACHKNP_RS17905"
                     /old_locus_tag="pgaptmp_003581"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HGU4705678.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(10469..11047)
                     /gene="lpcA"
                     /locus_tag="ACHKNP_RS17910"
                     /old_locus_tag="pgaptmp_003582"
     CDS             complement(10469..11047)
                     /gene="lpcA"
                     /locus_tag="ACHKNP_RS17910"
                     /old_locus_tag="pgaptmp_003582"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HGU4705679.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            11287..13731
                     /gene="fadE"
                     /locus_tag="ACHKNP_RS17915"
                     /old_locus_tag="pgaptmp_003583"
     CDS             11287..13731
                     /gene="fadE"
                     /locus_tag="ACHKNP_RS17915"
                     /old_locus_tag="pgaptmp_003583"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HGU4705680.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTASLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(13774..14220)
                     /gene="ivy"
                     /locus_tag="ACHKNP_RS17920"
                     /old_locus_tag="pgaptmp_003584"
     CDS             complement(13774..14220)
                     /gene="ivy"
                     /locus_tag="ACHKNP_RS17920"
                     /old_locus_tag="pgaptmp_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HGU4705681.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            14401..15168
                     /gene="yafV"
                     /locus_tag="ACHKNP_RS17925"
                     /old_locus_tag="pgaptmp_003585"
     CDS             14401..15168
                     /gene="yafV"
                     /locus_tag="ACHKNP_RS17925"
                     /old_locus_tag="pgaptmp_003585"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HGU4705682.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(15392..>15748)
                     /locus_tag="ACHKNP_RS17930"
                     /old_locus_tag="pgaptmp_003586"
     CDS             complement(15392..>15748)
                     /locus_tag="ACHKNP_RS17930"
                     /old_locus_tag="pgaptmp_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGU4705683.1"
                     /translation="CVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWH
                     VENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAA
                     MDRNYLASVLAGSGLS"
BASE COUNT         3666 a         4026 c         4236 g         3820 t
ORIGIN      
        1 caccctatta ataatttgcc cctttcacgc cccctctatt taatacctca ccttgtgtca
       61 ctgccggatt tgcgtgcaca aaatcgccca taccattaca gcagagaaag taagccatgc
      121 tgaagtacag taaaacgcca ggcatcttta aattagaagg taatcggctc ggacgcccct
      181 accatcgcct gccgaccctg tttacaggga attttgacgt gattgattcg catctgggca
      241 gctactttct caaaaagcat cgcagcaata tcaccctaaa aaagattggc tgcgaaatgg
      301 atattattaa taaaaacgct gaactgatgg tttcccaggt cgggcatctg gcgtttgata
      361 ttgatcgctc tttgctgtta atgctgctgg gcaattttta tggtctggag tcctctcttg
      421 aagaggcgaa agcgcataac gggctgccga ccaaaaccga aacgcggtta aaaaaccgtc
      481 tggcgctgga tatctgcacg ctgattttta atctgcaaac ctccggtatt gcgctgaaat
      541 taaagctgga cagcagcacg gtaattaccc actgggcgta tcaattaacc tttacccttg
      601 cgggcgatga agagagctgt tttcgtattt tgctcgacga cgcccacacc gattttattt
      661 taaatctgat ccgccacagc gagcacagta aaccgcagca ggtggcgaaa tcggtcaata
      721 aaccggccct gattaaagag atcatccgct ccctgccgct gaccctgaac gtcaaaatcg
      781 ccgagctatc gatgaatgtc gcggatctca cgcagataaa agccggggac attttaccca
      841 tctcgctggg agaaacgttc ccggtggcga ttgggcaatc tgaattattt agcgccctga
      901 ttgtggaaga caaagacaaa ctgtttttgt cggagctggc gggcaaaaat gagaatagcc
      961 atgagtaagc aagaagatat tttagccggg gattttggcc tcaccgatga agttgcgcct
     1021 gtcgcaaaat ccgccgacgc tgaaacgctg gtcacccgcc tggaagaccg tttttccgac
     1081 tcgatgacgc tgctaaaacg cattccggtg accttaacgc tggaagtgtc atcagtagaa
     1141 ataatgttag ccgacctcct taacatcgac gacgacacgg tgattgagct ggataaactc
     1201 gccggggagc cgctggatat caaagtgaac aatatcctgc tgggcaaagc ggaagtggtg
     1261 gtggtcaacg agaagtacgg cctgcgcgtg ctggaattta acacccgcga tatcaacgac
     1321 ctggcaccat gaaacgccgc actcaactga cgctgggcct tgggctgctg gcgcttgcgc
     1381 cggtggcgtt ggcccagggc ggcgatattg cgctgcttaa cgtggtcacc cacggcaaca
     1441 ctcaggagta cagcgtcaaa atccaggtgc tgattctgat gaccctcgtc ggtttgctgc
     1501 ctacgatggt gctgatgatg acctgcttta cgcgctttat tatcgtgctg tcgctgctgc
     1561 gccaggcgct agggctacaa caaacgccgc caaaccgcat tctgattggc attgcgctct
     1621 ccctcaccat gctggtgatg cgccccatct ggctcaatat ttacgaccac gcagtagtgc
     1681 cgtttgaaaa tgatcagatt accctgaccg atgcgttaag caccgccgcg acgccactca
     1741 aacgctttat gctggcgcag actgacaaaa aagcgatggc gcaaatcatg actatcggcg
     1801 gcgcgaaggg ccatgccgcc gaccaggatc tctcgattgt ggtgcccgcc tatgtgttga
     1861 gcgagctgaa aaccgccttc cagattggct ttatgattta catcccgttt ctggttatcg
     1921 acctgattgt cgccagcgta ctgatggcga tggggatgat gatgctgtcg ccgctgatcg
     1981 tttcgctacc ctttaagctg atgctgtttg tgctgatcga cggctggtcg ctgaccatcg
     2041 gtacactcac caccagcatt cgcgggctgg ggctgggctg atgatgctga ccatagacgt
     2101 agccgccgac attgtcgcca gcggcataaa agtggtgatt ttgctggttt cggtgctggt
     2161 agtgccgagc ctgctggtcg gcctgctggt gagcgtgttc caggcggtaa cgcagattaa
     2221 cgaacagacc ctaagctttt tgccgaggct gattgtcacg ctggtggtgc tgggcgtgtg
     2281 cggtaaatgg atgattatcc agttgcatga tctctgcatt cacctgttta cccaggccgc
     2341 gttgctggtt cattgagatg cgcacaagcg acgttacgca actgatggat ctggcgctgg
     2401 ggctgtggtt tccctttgtg cgcattatgg cgtttctgcg ctatgtgcct gtgctggata
     2461 acagcgccct gaccgtgcgc gtgcggatta ttttgtcgct ggcgctggca atcatcatca
     2521 cgccgttgat cccccatcct gttccccacg acctgatatc gctcaacagc ctgatcctga
     2581 cggtggagca aattttatgg ggcatgttat tcgggctgat gtttcagttt ttgtttctgg
     2641 cgctacagct ggccgggcaa attctctcgt tcaatatggg gatgagcatg gcggtgatga
     2701 acgacccgag cagtggcgca tcgaccaccg tgctggcgga gctcatcaac gtctacgcga
     2761 tcctgctgtt ttttgccatg gacgggcatc tgctgctggt gagtgtgctg tataagggct
     2821 ttacctactg gccgattggc aacgcgctgc atccgcaaac cctgcgcacc atcgcgctgg
     2881 cctttagctg ggtgctggcg tccgcatcgc tgctggcgct gccaaccact tttatcatgc
     2941 tgattgtgca gggctgcttt ggcctgctca accgcattgc gccgccgctg aacctttttt
     3001 cactcggttt tcccatcaac atgctggcgg ggctggtctg ctttgccacc ctgctctaca
     3061 acctgccgga tcactatctg catctggcga attttgtgtt gcagcaactc gacgcgctga
     3121 aaggtcacta tggcggatag cagcagcgaa gaaaaaacag agaaaccctc ggcgcaaaag
     3181 ctgcgtaaag cgcgcgaaga ggggcaactg ccgcgctcga aggatatggg gctggcggcg
     3241 agcctgtttg cggcatttgt ggtgatctcc agcagcttcc cgtggtatgc cgattttgtg
     3301 cgcgagagtt ttatcagcgt gcaccagtat gcacaggaga ttaataaccc agaggtgatc
     3361 ggccagtttc tgcgccatca cctgttaatt ctcggcaagt ttattctcac cttgctgccg
     3421 atgcccgccg ccgcgctact ctcctcacta gtgcccggcg gctggctgtt tctgcctaaa
     3481 aaaatcctcc cggatttcag caaaatcagc ccgttaaaag gcattgagcg gctgttttcc
     3541 agtgaacatc tggcggaaac gggaaagatg acggtgaagt cggtggtggt gctggtgatg
     3601 ctgtgggtaa gcctgcgcaa taactttgcc tcctttctcg gcctgcaggc gctaccgttc
     3661 aaactggcga tgaatgaggg tttatcgctc tacgcctgcg tgatgcgcaa ctttgtcatc
     3721 ctgtttatct tctttgcgtt aatcgacgtg ccgctggcga aagccctgtt taccaaaggg
     3781 ctgaagatga ccaaacagga gctgaaagaa gagtataaaa accaggaagg caagccggaa
     3841 gtgaaggcgc gcgtgcggcg tctgcaacgg cagttggcga tggggcaaat tcgcaaagtg
     3901 gtgccgaaag ccaacgtggt gatcaccaac ccgacccact acgccgtggc gttgcagtat
     3961 gactcgtcgc gcgccgccgc gccgtttgtg gtggcaaaag gcaccgatga aatcgccctc
     4021 tatattcgcc aggtcgccgc tgagaatcag gtggaagtgg tcgagttccc gcgactggcg
     4081 cgctcggttt actacaccac ccaggtgaac cagcaaattc cgtttcagct ttaccgggcc
     4141 atcgcccacg ttctgaccta cgtcctgcaa atgaaacact ggcgcgaggg tgcacagccg
     4201 cgcccggccc tgaacagaca tatttccatt cccaaagagg tgcttaaact ggatggcgaa
     4261 aacaactaaa tcgttcctcg cgctgctgcg cggcggcaat cttggcgtgc cgctggtgat
     4321 cctgtgtatt ctggcgatgg ttattttgcc gctgccgcct gcgctgctgg atattctgtt
     4381 caccttcaac attgtgctgg cggtgatggt gctgctggtg gcggtgtcgg cgaaacgacc
     4441 gctggaattc agcctgttcc cgaccatttt gctgatcacc accttaatgc gcctgacgct
     4501 caacgtcgcc tctacgcgcg tggtgctgct gcacgggcat ctcggcgcgg acgcggcggg
     4561 taaggtgatt gagtcgtttg gtcaggtggt gatcggcggc aactttgttg tcggctttgt
     4621 ggtgtttatc atcctgatga tcatcaactt tattgtcgtc accaaagggg ccgagcgtat
     4681 ttccgaggtt tcagcccgct ttaccctgga tgcgatgccc ggtaagcaga tggcgattga
     4741 cgccgatctt aatgccgggt tgatcaatca ggcgcaggca caaacccgac gtaaagatgt
     4801 tgccagcgag gccgatttct acggcgcgat ggacggggca tcgaaatttg tgcgcgggga
     4861 tgctatcgcc gggatgatga ttctggcgat caacctgatc ggcggcgtct gtatcgggat
     4921 cttcaaatac aacctgagcg ccgacgccgc cttccagcag tatgtgctga tgaccatcgg
     4981 cgacgggctg gtggcgcaaa tcccttccct gctactctcc accgcggcgg cgattatcgt
     5041 cacccgcgtc agcgacaacg gcgatatcgc ccatgacgtg cgtcaccagt tgctggcaag
     5101 cccatcggtg ctctacaccg ctaccggaat tatgttcgta ctggcggtag tgcctggaat
     5161 gccacatctg ccgtttttgc tgttcagcgc cctgcttggc tttaccggct ggcggatgag
     5221 caaacgcccg caggcggcgg aagcggaaga gaaaagtctc gaaacgctga cccgcaccat
     5281 caccgaaacc agcgagcaac aggtcagttg ggaaaccatt ccgctgatcg agcccatcag
     5341 cttaagcctc ggctacaagc tggtggcgct ggtggacaaa gcccagggca acccgctcac
     5401 ccagcgtatt cgcggcgtgc gacaggtgat atccgacggc aacggcgtgc tgctgccgga
     5461 gatccgcatt cgggaaaact tccgccttaa gcccagccag tacgctattt tcatcaacgg
     5521 cattaaggct gatgaagcgg atattccggc ggataaactg atggcgctgc cctccagcga
     5581 aacctacggc gagatagacg gcgtgctggg gaacgacccg gcgtacggga tgccggtgac
     5641 ctggatccag cctgcgcaga aagcgaaggc gctgaatatg gggtatcagg tgatcgacag
     5701 cgccagcgtg attgctaccc atgtaaataa gattgtgcgc agctatattc ctgatttatt
     5761 taactatgac gatattacgc agttgcataa ccgtttggcg tcgatggcac cgcgcctggc
     5821 ggaggattta agcgcggtgc tcaattacag ccagttgctg aaagtgtacc gggcgctgct
     5881 gaccgaaggc gtttccctgc gcgatatcgt caccatcgcc accgtgctgg tcgccagtag
     5941 cgcggtgacc aaagatcata ttctgctggc agccgatgtg cgcctggcgc tgcggcgcag
     6001 cattacccat ccgttcgttc gcaagcagga gctgacggtg tataccctga ataatgagct
     6061 ggaaaatctg ctgactaacc tagtgaatca ggcgcaacag ggcggtaaag tgatgctcga
     6121 cagcgtgcca gtggacccga atatgctcaa ccagttccag agtacgatgc cacaggtgaa
     6181 agagcagatg aaagcggcgg ggaaagaccc ggtgctgctg gtgccaccgc agctgcgccc
     6241 tttgctggcg cgttatgcaa ggttgtttgc gccggggctg catgtgctgt cgtataacga
     6301 agtgccggat gagctggagt tgaagattat gggggcgttg agttgatggg ggattctttt
     6361 ccatgctttg cagcacctga caccattaat tgacgtcaca ctgcaaagag actacgatga
     6421 caccatacaa tgacgtcatg gattgatagt atgattaagg tcagcgcgct aggttctcgt
     6481 catagaaaaa cattcgacga tgtaatgacg gagccgccaa aatctggaat taaatgggat
     6541 gatgttgttg cactcattaa agctgtgggt ggaacaataa aaaataacaa cggctccaga
     6601 cgaaaatttc agattggaac gaccaaattc agcacacatg agccacatcc aaaaaatgtg
     6661 atggataaag gcgcagtagc aggtcttaag gagtggtttg ttaactgtgt aggggtagat
     6721 tatgaataaa gcatcgaaca ttatcacaat taaaggtcaa ccagccgcag taacatttga
     6781 agccgatatc aacgcatttc gcggcaagtt cctcaatgta aacggttatt gtgactttgt
     6841 agccacaagt attgaggcat tatatcgtga aggcgaaagc gcgctggatg agtggatggc
     6901 ggattgtgaa gaggacggta ttgccccttt tcgtgaagaa gaagagcaaa aacgcctgac
     6961 gcttcgtgtt cctcatcaca ttgattcgcg gctgaccatc gtcgccagac aacattccat
     7021 ttcaaaaaac cagctcattg ttgatgtctt agaacgtgag ttttcagccc agatgtaaac
     7081 aggtcggatg ccgcgtggca tccgaccttt tgccagttcg tcaatattac ccatttacaa
     7141 aatcgtctcc tccgtcaaaa tccgtcgcgc gcctaaaaag tgatcctgcc agaaaggttc
     7201 agctaactga cttacccgaa tggtttcccc ggtgcgcggt gactcaataa actgaccgtc
     7261 gcccagatac acccccatat ggtcggcaat ctcgcggctg tggatatgga aaaatagcaa
     7321 atctccccgg cgcaggtcgt tattcgccac aatggttgcc cggcgatagt gatacatctc
     7381 attagccgtg cgtgggagtt tcgcctcaag gatcttgttg taggcataaa aaactaaccc
     7441 gctacagtcg aagccttgat cgggccgcgt accgccccag acgtaaggct tgccaagctg
     7501 ttgctccaga cggtgaatcg ccacttcggt gatgttatgc agatggtcgc tgcttaagaa
     7561 atggttgttt tccgccagcg cctgccagcg tctgtcactc gccttgagcg gcgcaggaaa
     7621 ccattctgga tgctgtttta tctgttcgcg gttgtgctgg cgtagcgccc ttctgctttc
     7681 ggcattgcct tccacaatat agctggcctg ttttttcaga tgggtttggt actgttttaa
     7741 taaacgcgca cgattttgca tccgctggcg ggcggcgtag ctgaaagtga tatgtgatgt
     7801 ggtggcgctg gcaaatgaag aaaacggcaa gcaaagcaac agtaatcccg acaacacaaa
     7861 agagggaatc gacggcaagg acatactcaa taatggctga tcatgcattg atgttgcagt
     7921 gtatcccggc ccgtacctga acaaacttaa taaaccatta ttataaaatt aataaatata
     7981 tttcagttaa ttatgtaaaa acatccgttt attaaattac tcatggattg tcgctgttgc
     8041 tcatttgagc tacaattcaa gctgaataaa tatacagcac aggagatacc ccaatggctg
     8101 ctaacgcatt tgttcgcgcc cgaatcgatg aagatctgaa gaatcaggcg gcggacgtac
     8161 tggccgggat ggggctgacc atctctgacc tggttcgcat aaccctcaca aaggtcgcgc
     8221 gtgaaaaggc attgccgttt gatttacgcg agcctaatca attaaccatt caatcaatca
     8281 aaaacagcga agctggcgtt gatgttcata aggccaaaga cgccgatgat ttatttgata
     8341 aattaggaat ttaaatcaaa gggatattga atactcggga caattttcaa aggatgtaaa
     8401 acttgcacaa aagcgtcata aggatatgaa taaattgaaa tatcttatga cgcttcttat
     8461 caataatgct ttaccgcttc cagctgttta taaagaccac ccgctgcaag gttcatggaa
     8521 aggttatcgc gatgctcatg tcgaaccgga ctggatcctg atttacaaac ttaccgataa
     8581 acttttacga tttgagagaa ctggaactca cgcggcgctc tttgggtaaa gcacgctgcc
     8641 gatgaaattt gtaaaccctg cgccgacagg cttgcgtaaa actcttttgg tgataatgtg
     8701 agccttccaa atccagacta ttccgatttg ggatctattt tgccgtcctg gcgtgtgtaa
     8761 ccgttttatc aaggaataag cagtatgcgt aaaatcgcat taattcttgc gatgttgttg
     8821 atcccgtgcg tttcgtttgc cggtttgctg ggcagcagta gctcgaccac gccagtgagc
     8881 aaagagtata agcagcagtt gatgggatcc cctgtctaca tccagatctt caaggaagaa
     8941 cgtacgctcg atctctacgt caaaatgggc gagcaatatc agctgctcga cagttataaa
     9001 atctgtaaat attccggcgg cttagggcca aaacagcgtc agggcgattt caaaagcccg
     9061 gaagggtttt atagcgtcca gcgtaatcag ttaaaaccag acagccgtta ctacaaagcg
     9121 attaatattg gtttccccaa tgcctatgac cgtgcgcatg gttatgaagg gaaatacctg
     9181 atgatccacg gcgattgtgt ttccatcggc tgctacgcaa tgaccaatca gggtattgat
     9241 gagatcttcc agttcgttac tggtgcgctg gtgtttggtc agccgagcgt gcaagtgagt
     9301 atttacccgt tccgcatgac cgacgccaat atgaagcgcc ataaatattc caactttaag
     9361 gacttctggg agcagctgaa gccgggctac gactactttg agcaaacccg taagccaccg
     9421 accgtttctg tggtcaatgg ccgttacgtg gtcagcaagc cgttaagcca cgaagttgtg
     9481 cagccacaac tggcatcaaa ctacacgctc cccgaggcaa aataagcgcc attcgcctgg
     9541 cataatcttt tgccaggttt cattgcccgt cagcggctgt gttgcaatca ccgtgaccac
     9601 atcattcggt gtggtctgcg agctgaagtc gatttccaca tcctgatcca gcagcgttgc
     9661 cacgccaaac ggcgcgcggc gggtgatcca gtgtaaatta gtcgagcaat acgccatcac
     9721 atagcgcccg tcagaaagca gcatgttgaa aacgcccttc tgccgcagtt catccgccag
     9781 tgaggcgata tatttaaaca ctgccgccat gttgcccggt gtgcgcgggt aacgctgcgt
     9841 taatttatgc aggagccagc agaaggcttt ttcgctatcg gtttcaccga ccgggcggaa
     9901 gttgccggtt tccagtgatt tgtagcccgt cagttgtccg ttatgggcat aagtccagtt
     9961 acgcccccat aactcgcggg taaatgggtg agtattttcc agcgccacct cgccccgatt
    10021 agcctggcga atatgagcca ccaccgaaca ggattttatc gggtagtcct ggacaagttt
    10081 ggcgatgggg gaattaaagc tgggttgtgg atctttaaat gtgcgacagc ctttaccttc
    10141 gtaaaaggta atgccccagc catctttatg tggcccggtt ccaccaccac gctgtacaag
    10201 tccggtgaaa ctaaagcaga tatcggtagg gacgttggcg ctcatcccga gcaattcgca
    10261 catgggtaac ctccacaaca gcgggagcga atggcccccg gcgaagattg atttaagaat
    10321 tttcaggtcg gatgcggcgt aaacgaatta ttcggcctac ggttcgggta cagatttgta
    10381 ggcatgataa gacgcgtcag catcgcatca ggcatcagcg cacaagtgcc ggatgcggcg
    10441 taaacgcctt atccagccta cgccagactt acttaaccat ctctttttca attaactgga
    10501 tcaggatatg gatcacttta atgtgaatct cctgaatgcg gtcggcataa ccaaagtgcg
    10561 gtacgcgaat ttcgatatcc gccgtgccag ccattttgcc gccgtcttta ccggtcaggg
    10621 tgatcacttt catccccttc tcacgcgccg ctgcgatcgc tttgatcacg tttgcagagt
    10681 taccggaggt ggagatcccc agcagtacat cgccttcgcg acctaccgct tcaacgtagc
    10741 gggagaaaat atcattgaaa ccgaaatcat taccgacgca ggaaatatga ctaacgtcag
    10801 aaatagcaat cgccgggtag cccggacggt tttcacggta gcgaccggtc aactcttcgg
    10861 caaagtgcat agcgtcgcaa tgggaaccgc cgttgccgca ggaaagcact ttgccaccgg
    10921 ctttaaagct gtctgctaac aggaccgccg cgcgctgaat ggcgtgaata ttggcgtcat
    10981 cttttaaaaa gttagccagc gtttccgccg cttcgttcag ttcgttacga ataagatcct
    11041 ggtacatgag gatatccttc agcataaatg taatagacaa aatgcagtgt accggatacc
    11101 gccaaaagcg agaagtacgg gcaggtgcta tgaccaggac tttttgacct gaagtgcgga
    11161 taaaaacagc aacaatgtga gctttgttgt aattatattg taaacacatt gctaaatgtt
    11221 tttacatcca ctacaaccat atcatcacaa gtggtcagac ctcctacaag taaggggctt
    11281 ttcgttatga tgattttgag tattctcgct acggttgtcc tgctcggcgc gttgttctat
    11341 caccgcgtga gcttatttat cagcagtctg attttgctcg cctggacagc ctccctcggc
    11401 gttgctggtc tgtggtcggc gtgggtactg gtgcctctgg ccattatcct cgtgccattt
    11461 aactttgcgc ctatgcgtaa gtcgatgatt tccgcgccgg tatttcgcgg tttccgtaag
    11521 gtgatgccgc cgatgtcgcg cactgagaaa gaagcgattg atgcgggcac cacctggtgg
    11581 gagggcgact tgttccaggg caagccggac tggaaaaagc tgcataacta tccgcagccg
    11641 cgcctgaccg ccgaagagca agcgtttctc gacggcccgg tagaagaagc ctgccggatg
    11701 gcgaatgatt tccagatcac ccatgagctg gcggatctgc cgccggagtt gtgggcgtac
    11761 cttaaagagc atcgtttctt cgcgatgatc atcaaaaaag agtacggcgg gctggagttc
    11821 tcggcttatg cccagtctcg cgtgctgcaa aaactctccg gcgtgagcgg gatcctggcg
    11881 attaccgtcg gcgtgccaaa ctcattaggc ccgggcgaac tgttgcaaca ttacggcact
    11941 gacgagcaga aagatcacta tctgccgcgt ctggcgcgtg gtcaggagat cccctgcttt
    12001 gcactgacca gcccggaagc gggttccgat gcgggcgcga ttccggacac cgggattgtc
    12061 tgcatgggcg aatggcaggg ccagcaggtg ctggggatgc gtctgacctg gaacaaacgc
    12121 tacattacgc tggcaccgat tgcgactgtg cttgggctgg cgtttaaact ctccgacccg
    12181 gaaaagctgc tgggtggcgc agaagattta ggtattacct gtgcgctgat cccgaccacc
    12241 acgccgggcg tggaaattgg tcgtcgccac ttcccgctga acgtaccgtt ccagaacgga
    12301 ccgacgcgcg gtaaagatgt cttcgtaccg atcgattaca tcatcggtgg gccgaaaatg
    12361 gccgggcaag gctggcggat gctggtggag tgcctctcgg taggccgcgg catcaccctg
    12421 ccttccaact caaccggcgg cgtgaaatcg gtagcgctgg caacgggcgc gtatgctcac
    12481 attcgccgtc agttcaaaat ctctatcggt aagatggaag ggattgaaga gccgctggcg
    12541 cgtattgccg gtaatgccta cgtgatggat gctgcggcat cgctgattac ctacggcatt
    12601 atgctcggcg aaaaacctgc cgtgctgtcg gctatcgtta agtatcactg tacccaccgc
    12661 gggcagcagt cgattattga tgcgatggat atcactggtg gcaaaggcat tatgctcggg
    12721 cagagcaact tcctggcgcg tgcttaccag ggcgcaccga ttgccatcac cgttgaaggg
    12781 gccaacattc tgacccgcag catgatgatc ttcggacaag gagcgattcg ttgccatccg
    12841 tacgtgctgg aagagatgga agcagcgaag aacaatgacg tcaacgcgtt cgataaactg
    12901 ttgttcaaac atatcggtca cgtcggtagc aacaaagttc gcagcttctg gctgggcctg
    12961 acgcgcggtt taaccagcag cacgccaacc ggcgatgcca ctaaacgcta ctatcagcac
    13021 ctgaaccgcc tgagcgccaa cctcgccctg ctttctgatg tctcgatggc tgtgctgggc
    13081 ggcagcctga aacgtcgtga gcgcatctcg gcacgtctgg gggatatttt aagccagctt
    13141 tacctcgcct ctgccgttct gaagcgttat gacgacgaag gccgtaatga agccgacctg
    13201 ccgctggtgc actggggcgt acaagatgcg ctgtatcagg ctgaacaggc gatggatgat
    13261 ttactgcaaa acttcccgaa ccgcgtggtt gccgggctgc tgaatgtggt gatcttcccg
    13321 accggacgtc attatctggc accttctgac aagctggatc ataaagtggc gaagatttta
    13381 caagtgccga acgccacccg ttcccgcatt ggtcgcggtc agtacctgac gccgagcgag
    13441 cataatccgg ttggcttgct ggaagaggcg ctggtggatg tgattgccgc cgacccaatt
    13501 catcagcgga tctgtaaaga gctgggtaaa aacctgccgt ttacccgtct ggatgaactg
    13561 gcgcacaacg cgctggcgaa ggggctgatt gataaagatg aagccgctat tctggtgaaa
    13621 gctgaagaaa gccgtctgcg cagtattaac gttgatgact ttgatccgga agagctggcg
    13681 acgaagccgg taaagttgcc ggagaaagtg cggaaagttg aagccgcgta attgaataac
    13741 ggagccgaaa ggctccgttt ctttatccgc taattattta aaattaaagc catccggatg
    13801 gttttccagg ctgccggtca acgccgcgaa cagcaccgtt ttaccatcaa tcgaaagcgc
    13861 atcgttcaca ttcagccagg tgagtttctc ttgcgacgtt ttctcatcaa tagtcgagaa
    13921 cagccccgtc atctgattag atttctcgga ccacatcaca gcgatacgtt gcgagccaca
    13981 gtcatgcggt ttgcacgcgc tcatcacctg atacgtctca tctcccaacg ttacggtttg
    14041 tgcgggagta taagtaccgc ctttcatcac ccaggcaggc agcttatgcc cttgtaccat
    14101 ctgattaaat gcagctttgg tggtttcgcc ctttgcaagg ctgctaatgg ttaaatcatc
    14161 ctgcgccatt gcactggtgg cgatgaccag agcggcgact gtcgttattg ccttaaacat
    14221 cattcctccc gagcttatcc tgcccatgtt attaaccttc taaagcctgg cgataacacg
    14281 ataatttgca aatcagatat ttccaatatc agcatttgtt atcaatttat tctgcgctaa
    14341 ggcaactgcc tgataaggtg ttaaagttat aaaacccttc gcataaagga gttgtacacc
    14401 gtgcctggtt tgaagattac gcttttgcag caaccactgg tgtggatgga tggccctgcc
    14461 aacctgcgtc attttgatcg tcaactggaa ggtattaccg ggcgcgatgt gatcgttcta
    14521 ccggagatgt ttaccagcgg ctttgccatg gaagcggcag cttcgtcgct agcacaagat
    14581 gacgtagtga actggatgac agctaaggcg cagcagtgca atgcgctgat tgcaggcagt
    14641 gttgcattac aaacggagtc tggttcggtt aaccgctttt tgctggttga gccgggcggc
    14701 acggtacatt tttatgataa gcgtcatctg ttccgcatgg cagatgagca tctacattat
    14761 aaagcgggca atgcgcgagt gattgtggaa tggcgcggct ggcgtatttt gccgctggtg
    14821 tgctacgact tacgttttcc tgtgtggtcg cgcaatctca acgattatga cctcgccctg
    14881 tacgtcgcca actggcctgc tccgcgctct ctgcactggc aggcattgct gacggcccgc
    14941 gcgattgaga atcaggcgta tgtggcggga tgcaatcgcg tcggcagcga tggcaacggc
    15001 tgccattatc gcggtgacag ccgggtgatt aatccacaag gcgagattat cgctactgcc
    15061 gacgcgcatc aggcaacgcg cattgatgcc gagctgtcga tggcggtgtt aagggaatat
    15121 cgcgataagt ttccggcatg gcgggatgcg gatgagttta ggttgtagtg agtgggggct
    15181 gtatttaggg cggggaattc agaaagggat gtttagtgtg ctgagcgaga gttaaataat
    15241 aaaaaactgg gaagatttaa taatcccccc gcagggcaag agcatgaatt aaatttgtgt
    15301 taaatttttc attttatcag ttcgaaaccc aagctctgag caggatgcaa gcacttcttc
    15361 tatataacat gtgtttaacg ccagggcaag attacgaaag cccgctcccc gcaaggactg
    15421 acgccagata gtttctgtcc atggctgctt ttcgcatctt acgtcttaac cctgccttga
    15481 ataccttatc attcgtcaaa atattaatag caatgtgccg tatccctgaa aataattctg
    15541 ctgcatttcc tcttcttatt ttgcagtcgt cttcattcat taccacgtcc agacgccagt
    15601 gcagcttatt ctccacgtgc cagtgatttc ggatcgctgt ggcgaatttc tcagcggtta
    15661 aatcagcaga actgatataa tatctgaccg tcatttctgg ctctttcttt tgttctgcta
    15721 ttatggaccg aaaggagact gccacgca
//
LOCUS       DAZFWL020000107        15727 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFWL020000107 DAZFWL020000000
VERSION     DAZFWL020000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.107"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            134..376
                     /gene="yjbE"
                     /locus_tag="ACHKNP_RS17935"
                     /old_locus_tag="pgaptmp_003587"
     CDS             134..376
                     /gene="yjbE"
                     /locus_tag="ACHKNP_RS17935"
                     /old_locus_tag="pgaptmp_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HGU4705684.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            490..1128
                     /locus_tag="ACHKNP_RS17940"
                     /old_locus_tag="pgaptmp_003588"
     CDS             490..1128
                     /locus_tag="ACHKNP_RS17940"
                     /old_locus_tag="pgaptmp_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HGU4705685.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1125..1862
                     /locus_tag="ACHKNP_RS17945"
                     /old_locus_tag="pgaptmp_003589"
     CDS             1125..1862
                     /locus_tag="ACHKNP_RS17945"
                     /old_locus_tag="pgaptmp_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU4705686.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1862..3958
                     /locus_tag="ACHKNP_RS17950"
                     /old_locus_tag="pgaptmp_003590"
     CDS             1862..3958
                     /locus_tag="ACHKNP_RS17950"
                     /old_locus_tag="pgaptmp_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU4705687.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKVAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4005..4283)
                     /locus_tag="ACHKNP_RS17955"
                     /old_locus_tag="pgaptmp_003591"
     CDS             complement(4005..4283)
                     /locus_tag="ACHKNP_RS17955"
                     /old_locus_tag="pgaptmp_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705688.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4284..4427
                     /locus_tag="ACHKNP_RS17960"
                     /old_locus_tag="pgaptmp_003592"
     CDS             4284..4427
                     /locus_tag="ACHKNP_RS17960"
                     /old_locus_tag="pgaptmp_003592"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705689.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4553..4963
                     /gene="psiE"
                     /locus_tag="ACHKNP_RS17965"
                     /old_locus_tag="pgaptmp_003593"
     CDS             4553..4963
                     /gene="psiE"
                     /locus_tag="ACHKNP_RS17965"
                     /old_locus_tag="pgaptmp_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HGU4705690.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5007..6482)
                     /gene="xylE"
                     /locus_tag="ACHKNP_RS17970"
                     /old_locus_tag="pgaptmp_003594"
     CDS             complement(5007..6482)
                     /gene="xylE"
                     /locus_tag="ACHKNP_RS17970"
                     /old_locus_tag="pgaptmp_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HGU4705691.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLHTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDATWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNALAAQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVTHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6854..7744)
                     /gene="malG"
                     /locus_tag="ACHKNP_RS17975"
                     /old_locus_tag="pgaptmp_003595"
     CDS             complement(6854..7744)
                     /gene="malG"
                     /locus_tag="ACHKNP_RS17975"
                     /old_locus_tag="pgaptmp_003595"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HGU4705692.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7759..9303)
                     /gene="malF"
                     /locus_tag="ACHKNP_RS17980"
                     /old_locus_tag="pgaptmp_003596"
     CDS             complement(7759..9303)
                     /gene="malF"
                     /locus_tag="ACHKNP_RS17980"
                     /old_locus_tag="pgaptmp_003596"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HGU4705693.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTTQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9457..10647)
                     /gene="malE"
                     /locus_tag="ACHKNP_RS17985"
                     /old_locus_tag="pgaptmp_003597"
     CDS             complement(9457..10647)
                     /gene="malE"
                     /locus_tag="ACHKNP_RS17985"
                     /old_locus_tag="pgaptmp_003597"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HGU4705694.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11012..12127
                     /gene="malK"
                     /locus_tag="ACHKNP_RS17990"
                     /old_locus_tag="pgaptmp_003598"
     CDS             11012..12127
                     /gene="malK"
                     /locus_tag="ACHKNP_RS17990"
                     /old_locus_tag="pgaptmp_003598"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HGU4705695.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12199..13539
                     /gene="lamB"
                     /locus_tag="ACHKNP_RS17995"
                     /old_locus_tag="pgaptmp_003599"
     CDS             12199..13539
                     /gene="lamB"
                     /locus_tag="ACHKNP_RS17995"
                     /old_locus_tag="pgaptmp_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HGU4705696.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13782..14702
                     /gene="malM"
                     /locus_tag="ACHKNP_RS18000"
                     /old_locus_tag="pgaptmp_003600"
     CDS             13782..14702
                     /gene="malM"
                     /locus_tag="ACHKNP_RS18000"
                     /old_locus_tag="pgaptmp_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HGU4705697.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14931..15727
                     /locus_tag="ACHKNP_RS18005"
                     /old_locus_tag="pgaptmp_003601"
                     /pseudo
     CDS             14931..15727
                     /locus_tag="ACHKNP_RS18005"
                     /old_locus_tag="pgaptmp_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT         3964 a         3966 c         4076 g         3721 t
ORIGIN      
        1 attcattcca attatcctaa aacgccatcg ctaattcccc gcgccgtaat tcgcatgctt
       61 tagttgtgta tactcgatcc cgcccgaaat gtttttgggt aaatctccat tcattcaatg
      121 aagggaaatt gttatgaaaa aagttctgta tggcattttt gccatatctg cgcttgcggc
      181 gacttctgcg tgggctgcac ctgtacaggt gggcgaagcg gcagggtcgg cagcaacgtc
      241 ggtttcggcg gggagttcct ccgctaccag cgtcagcacc gtaagctcgg cggtgggtgt
      301 cgcgctcgcg gcaaccggtg gcggtgatgg ttctaatacc gggaccacca caaccacgac
      361 caccagtacc cagtaataaa gtatgtatcc ccaaaataat ttgagtcatt gcatctgtgg
      421 cttgaagtat gaagggattt accataacca cactccggtg tggttattct gcccctctgg
      481 agaagagtcg tgaagcgacc tgcactcatt cttatctgcc tgctattaca ggcctgttca
      541 gccacgacta aagagctggg caattcactg tgggacagtc tgttcggcac gccaggcgta
      601 cagctgacgg acgatgatat tcaaaatatg ccctacgcca gccagtacat gcagcttaat
      661 ggcgggccgc aattgtttgt ggtgctggcc ttcgctgaag acggacaaca aaaatgggtc
      721 actcaggatc aagccactct tgtcacacaa catggtcgtc tggtgaagac tttgcttggc
      781 ggcgacaacc tgattgaagt gaataacctt gccgccgacc cgctgattaa acccgcacaa
      841 attgttgatg gtgcaacatg gacgcgcacg atgggctgga ccgagtacca gcaggtacgc
      901 tacgccaccg cacgctcagt cttcaaatgg gatggcaccg ataccgtcaa agtcggcagc
      961 gatgaaaccc tggttcgcgt gctggacgaa gaagtctcca ccgaccaggc gcgctggcat
     1021 aaccgctact ggatcgacag cgaagggcaa attcgccagt cggaacagta tctcggcgcg
     1081 gattatttcc cggtgaaaac cactctcatc aaggcggcaa aacaatgatt aaacaaactt
     1141 ttgtcgcgtt gcttttgagt gtgggagcgt catcggtctt cgcggcagga accgtcaagg
     1201 tgttcagcaa tggcagcagt gaggccaaaa cactaacagg cgcagagcat ttaatcgatc
     1261 tggtaggcca accgcggctg gcaaacagct ggtggcccgg tgcggtgatt agcgaagagc
     1321 tggcaacggc ggcagcattg cgtcagcagc aggcgttgct gacacggctg gcagaacagg
     1381 gcgcagattc cagcaccgac gatgccgccg ccattaacgc cttacgccag caaatccagg
     1441 cattgaaagt gacaggcagg caaaaaatca atcttgatcc cgatatcgtc cgcgttgccg
     1501 aacgcggtaa cccgccgttg cagggcaact acacgctgtg ggtcggacca ccgccgtcca
     1561 cggtcacgtt gttcgggctt atcagccatc ctggcaatca gccattcact ccaggtcgcg
     1621 acgtggcgag ctatctctcc gggcagaacc tgcttagcgg tgcggatcgc agctacgcgt
     1681 gggtcgttta cccggacgga cgcacgcaaa aagcgccggt ggcttactgg aacaagcgtc
     1741 acgtagagcc gatgcccggc agcattattt atgttggcct cgcggactcc gtctggagtg
     1801 agacgcctga tgcccttaac gccgacattc ttcagactct gacgcagcgg ataccccaat
     1861 aatgaaaaaa agacatctgc ttagcttact ggcgctgggc attagcatgg cttgctacgg
     1921 cgaaacatat cctgcgccca ttggcccgtc gcagtcggat ttcggtggcg taggattatt
     1981 acaaactccc accgcgcgta tggcacggga aggggagttg agtctgaact atcgcgataa
     2041 cgatcagtac cgttattact cagcttcagt gcaactcttc ccgtggctgg aaacaacgct
     2101 gcgctacacc gacgtgcgca cccggcagta cagcagcgtc gaagcgttct ctggcgatca
     2161 aacgtataaa gataaagcct tcgatctcaa actgcgtttg tgggaagaga gttattggct
     2221 gccgcaagtg gcggttggtg cgcgggatat cggcggtacg gggctgtttg atgcggaata
     2281 tcttgttgcc agcaaagcct gggggccgtt cgattttacg ctcggcctgg gctggggata
     2341 tctgggcacc agcggtaatg tgaaaaatcc gctctgttca gccagtgata aatattgcta
     2401 tcgcgataac agctacaaac aggcgggatc tatcgacggt agccagatgt tccacggtcc
     2461 tgcctcactg tttggcggcg tggaatacca gacgccctgg caaccgctgc gcctgaaact
     2521 ggagtatgaa ggcaataatt atcagcagga ttttgccggg aagctggagc aaaaaagtaa
     2581 gtttaacgtc ggtgcgattt atcgcgttac cgattgggcc gacgttaacc ttagctatga
     2641 acgtggcaac acctttatgt ttggcgttac gttgcgcacc aactttaacg atctgcgccc
     2701 gtcttacaac gataacgccc gcccgcaata tcaaccgcag ccgcaggatg ccattttgca
     2761 gcattcggtg gtggcgaatc agttaacgtt gctgaaatac aatgccggac ttgccgatcc
     2821 gcagatccag gcgaaaggcg atacgctgta tgttaccggc gagcaggtga aatatcgtga
     2881 ttcgcgcgaa gggatcatcc gtgcgaatcg gatcgtgatg aacgatctgc cggatgggat
     2941 caaaacgatc cgcattacag aaaatcgcct taacatgccg caggtgacga cggaaaccga
     3001 tgtcgccagc ttgaaaaatc atctcgccgg ggagccgctg ggccatgaaa cgaaggtggc
     3061 gcaaaaacgc gtcgagccag tggttccgaa gtccaccgag cagggatggt atatcgacaa
     3121 atcacgcttt gatttccata tcgatccggt gctgaaccag tcggtcggtg gcccggaaaa
     3181 cttttacatg tatcagctgg gcgtgatggg aacggcagat ttgtggctga cggaccatct
     3241 gctgaccacc ggcagcctgt ttgcaaatct tgccaacaac tacgacaagt ttaactacac
     3301 caatccgccg caggactcgc acttaccgcg cgttcgtacc catgtgcgcg agtatgtgca
     3361 gaacgatgtc tatgtgaata acctgcaagc caactacttc cagcatctgg gcaacggctt
     3421 ctacggtcag gtctatggag gttatctcga aaccatgttt ggcggtgccg gggcagaagt
     3481 gttgtatcgt ccgctggaca gcaactgggc gtttggtctg gatgccaact acgttaaaca
     3541 gcgcgactgg cgtagtgcaa aagatatgat gaaattcacc gactacagcg tgaaaaccgg
     3601 acatctgacc gcctactgga cgccatcttt cgcccaggac gtgttggtga aagcgagcgt
     3661 cgggcagtat ctggcagggg ataaaggcgg cacgctggag atcgccaaac gctttgatag
     3721 cggcgtggtg gttggcggct atgccacgat cacgaatgtt tcgaaagagg agtacggcga
     3781 aggggacttc accaaaggcg tgtatgtctc tgtgccgttg gatctcttct cgtctggccc
     3841 gacacgcagc cgtgcggcga ttggctggac gccgctgacg cgtgacggtg gtcagcaact
     3901 tgggcgtaag ttccagttgt atgacatgac cagcgaccgt agcgtcaatt tccgctaagt
     3961 catgggaaag gtgccagttt tcgcaatcac tggcaccctc attcttaatg gcctgttgag
     4021 ccatcaggaa cgcagttcac ccccggtttg cgatctggtg tgtaaaggcg aaaacattct
     4081 gtcttcctgt ccgtcgatat cggttgcaga tctggttgcc tgagcgactg ggccatcatt
     4141 gggctggaga cattgctttg cggtaggtcg gctttatcag cagtaccgag cggaccagca
     4201 taagcaggaa gaacagagac tgataacatc aaagcagcaa aataaggctt catttttacc
     4261 acctttatca ggttacgttt catttgttcc agaggaacat tgtcgatttt tcgcgcattg
     4321 ctggtggctg ggaatcacct gaatgggtga tttttgaatt accggctttg gtgcggtttg
     4381 tcttgccgga tgcgccgcca ggcgcggctt atccggccta cgggtaggta tatccggctt
     4441 tggtggaggc gcgctccaaa tccaggttga acaaaacata cacaaaaaat atagatctcc
     4501 gtcacatttt tgcgttatac aggaagctcg ccactgtgaa ggaggtactg ctatgacgtc
     4561 actctctcgt ccgcgcgtgg agtttatctc caccattttg cagaccgtac tcaatcttgg
     4621 cctgttgtgc ctcggcctga ttttggttgt cttcctcggc aaagaaacgg tgcatctggc
     4681 tgatgtgctg ttcgcgccag aacaaaccag caaatatgag ctggtagaag ggctggtggt
     4741 ttactttctc tatttcgaat ttatcgcgct gattgtgaag tactttcagt ccggttttca
     4801 cttcccgcta cgttactttg tctatattgg gatcaccgcc attgtgcgct tgatcatcgt
     4861 cgatcataaa tcgccactgg atgtattgat ctactcggca gcgatcctgt tactggtgat
     4921 caccctgtgg ctgtgtaatt cgaaacggct gaagcgggag taaaaagaca gcacgccgaa
     4981 ctggcgcggc gtgctggaca ggaagattac agtgtagcag tttgttgtgt tttcttcgtt
     5041 tccggttccc agagggcttc cagctcctca agggttttac ctttggtttc cgggacaaat
     5101 ttccacataa atagtgctgc cagaacgccc atacaaccgt aaatccagta ggagaaaccg
     5161 ttgtggaaat gggtcaccag ccaggagttt ttgtccatca tcgggaaggt ccaggagacg
     5221 aagtagttcg ccagccactg ggccgccacc gcgattgcca gcgctttacc acgaatagca
     5281 ttcgggaaga tttccgacag cagtacccag cataccggac cccaggacat ggcaaaggca
     5341 gcaacataga acagcatcga cagtagcgcc acaatacccg gtgcctgagt gtaaaacgcg
     5401 gtaccgaggc taaacatacc gattgccatt ccgagcgcgc cgataatttg cagtggctta
     5461 cgaccaaatt tatccaccgt cataattgcc agaacggtga aggtgaggtt gataactccg
     5521 acaataatgg tctgcaacag cgcgatatcc gtgctggccc ccagcgtttt gaacacttcc
     5581 ggcgcgtagt acaacaccac attgatgccg acaaattgct ggaagatgga gagcataacg
     5641 ccgataacaa tcacgcccac gccgaacatc agcagacgac caccggtttt gcgtccatga
     5701 tccagggagt gtttaatttc ctgcaccgcc tgggctgcca aagcgttgcc cataattttg
     5761 cgcaggatgc cttccgcttg ttcttgctta ccgcgtgaca tcagccagcg agggctttcc
     5821 ggcacggtat aaagcagcat taagaacagc agcgcaggga tacattccga agcaaacata
     5881 taacgccagc cgtcagtatt cagccaggtg gcatcgccgg aacgggcaat aaaatagttt
     5941 acgcagtaaa ctaaaagttg gccgaaaata atcgcaaact ggttaaaaga gaccagcttc
     6001 ccacgaatat gtgcaggagc cagttccgca atatacattg gtgagagcat tgaggctaaa
     6061 ccaacaccaa taccgccaat aatgcgataa ataacaaatt ccgggacata acctgccaga
     6121 taaacaggca ctgtgttgtc cgggtttata gaggtaaaac caagttctgg ccaggcagaa
     6181 cctacaccag aaataaaaaa caggacagca gcaatcttaa gtgaatcacg acgaccgaag
     6241 cggttactgc aataaccacc gagggcaccg ccgatgatgc aaccaatcag agcgctggct
     6301 acgcaaaacc ctaacaggga gttggcagcg gattcactta agttttgtgg agcaacaaag
     6361 acggtatgga gtgactcaac agtaccggaa ataacggcgg tgtcgtagcc aaataataaa
     6421 ccacctaatg tagcgactaa ggtaatcgaa aatatataac tggaattata ctgggtattc
     6481 attcagacct gccttagacc attctgatct tttatcagtg cattgatgtt ggaattgtca
     6541 ggcagttaag gcaattatca ttttttacaa tgcgtttacg taatgtcttt tctgtgatct
     6601 taattgtgat aattatctaa aattgatgaa aacaatacta ttgccgtgac tcagagcacg
     6661 aaagagaatt atcgtaagtg ggaaaacaaa taacgtaaaa ataataagct ctatgatggg
     6721 aatattaacc ggcgaacgat tcagattgca gacgaaagaa aaaaaggcgc tccgtggagc
     6781 gccgaataac agtcacaagt tgggataacg taagttgagg gtgcagcggc ataacattgg
     6841 cagaacaaca tctttaacct ttcacaccac ctgccgtcag gccgttgacc agccagcgtt
     6901 gagccagcaa gaagacgatg gtgatcggta atgcagacat cacggcagcg gcggcaaagt
     6961 caccccacag gtagttttgc gggttgaggt attgctgcat ccccacggcc agggtgtagc
     7021 tgtttacgtc acgcagtaac agcgacgcga ccggaacttc agtaatggca gcgataaacg
     7081 acaagatgaa taccaccgcc agaatcggta ctgacagcgg caacaggaca aggcggaagg
     7141 cctgccacgg tgtcgcacca tccagcgcag ccgcttcttc cagcgaacta tcgatggttt
     7201 cgaaatagcc tttgatggtc cagacatgca gcgcaatccc acccagatac gcgaaaatta
     7261 cgccgccgtg agtattcagg ccaatgaatg gaatgtactc acccagacga tcaaacaacg
     7321 catacaacgc gaccagtgaa agtactgccg ggaacatctg gaaaatcagc attcctttca
     7381 gcagcgtcgc tttgcctgga aagcgcatac gggcgaaagc gtaggcgcag gtggtagaga
     7441 gcgccacaat gccaatcgcg gaaatcccgg cgacctttac cgagttccac agccacagca
     7501 gtaccgggaa tggcggtggc gtaatgcgac catcagcctg ttcaacgcta aaacctaacg
     7561 ccagtttcca gtgatcccag gagatttgct ccgggatcag gctaccggtc gcgaagtttc
     7621 cctgacgcag cgagatagcg acgaccatca gcagcgggaa cataatcgcc gcgataaaaa
     7681 gtagcagtag caggtgagta ataaacaaac gtgctttttg cgatttcggt tggaccattg
     7741 ccatttttgt tatctccctt aatcaaactt cattcgcgtg gctttcaggt tcactatcgc
     7801 cagcgcaccc accagcagga agatcagcgt ggcaattgct gctgccagac cgaagtcctg
     7861 acccccgccg ccttcaaaag cgatgcggta ggtgtagtta acaagcaggt cggtataacc
     7921 ggctggcgtg gtcgtgccaa gacgatccgg gccgccgttg gttaacagtt gaatcagcac
     7981 gaagttgtta aagttaaagg cgaagctggc gatcatcagc ggcgtcagcg gtttaatcag
     8041 cagcggcagc gtaatcttaa agaagttctg gaacggacct gcgccatcca ttgctgaggc
     8101 ttcatacaaa tcgtccggaa tcgctttcag caagcccatg cagaggatca tcatgtacgg
     8161 ataacccagc caggtattga cgataatcag catcgtgcgg gcggtggtcg gatcgctgaa
     8221 ccaggcgggc ttcacgccaa acagcgcgct caacatcatg ttgatttcac cgaagctctg
     8281 gttaaacaac cctttgaaaa tcaagattga aatgaacgat ggcaccgcgt agggcagaat
     8341 cagcaggacg cgatagaccg ctttgccgcg caacgcttcc cactgcacca gacacgccag
     8401 aaccatgccg accgccaccg ttaaaaagac agtgatcagc gagaacacca cggtccagac
     8461 gaaaatggcg aggaacggtt tctgaatgcc ttcgtcggtg aagacgcggg taaagttttt
     8521 ccagccagta gtcacggtgt aaccggggct tagcttttca tcgccccagt tgccgtcggc
     8581 ggtaatggac tggtaaaagc caatttggtt attcggacga tatttcacgc cgctctgatt
     8641 attcgtcaac gtgccgtcac cgtcgagtgt gtagagcggc tgcgtgccag aaaactggcg
     8701 cagggagctc atcatcactt tgttgccatc cggcagaatg gcggtaatgt cactcagcgc
     8761 ctgacgattc tgggtaatca cgcgcagatt cgcgcgttcg ccttcgggct gggtggtcgt
     8821 ttctttcagt tgcagttttt gctcgccgcc aaatttaaaa gcgtcggaga ggtaattttt
     8881 gccggtttcg ccgtcgctga gggctagttg ccactcatcg cccgccgggt aaagaccaaa
     8941 attataggtt ttgcctgctt gccaggagcg atctagcaac acttcctgcg cacgttcaaa
     9001 agtcagctgg ttagtgctgc tgtagttggt gaaggcaatg gcgatggtgc agaccagagg
     9061 gaagaggacg aataatccca ttccagccat tcccgggtaa acatagcgcc aggcgtaggc
     9121 tttacgattg gcgaaaatat acagccccgc tgaactcaat atcagcgtgg taatggcgaa
     9181 caggtattcc ccttgtgcgt acattaaaac aacaaggtaa cccaccagca ggccgagcag
     9241 acctaacact gaccatttca gcgcgtcgct ttgccaccaa tgtttctttt taatgacatc
     9301 catggggttc ttcctcattc caggacggat aaggctttca cgccttatcc gacaacaact
     9361 gcctgatgcg acgctgacgc gtcttatcag gcctacatac gtttcggttt tgtaggccgg
     9421 acaaggcgtt cacgccgcat ccggcatttc acagcattac ttggtgatac gagtctgcgc
     9481 gtctttcagg gcttcatcga cagtctgacg accgctggcg gcgttgatca ccgcagtacg
     9541 cacggcatac cagaaagcgg acatctgcgg gatgttcggc atgatttcac ctttctgggc
     9601 gttttccatg gtggctgcaa tacgtggatc tttcgccaac tcttcctcgt aagacttcag
     9661 cgctacggca cccagcggtt tgtctttatt aaccgcttcc agaccttcgt cagtcagcag
     9721 atagttttcg aggaactctt tcgccagctc tttgttcgga ctggcggcgt taataccggc
     9781 gctcagcacg ccaacgaacg gtttagatgg ttgacccttg aaggtcggca gtaccgttac
     9841 accataattc actttgctgg tgtcgatgtt ggaccatgcc cacgggccgt tgatggtcat
     9901 cgctgtttcg cctttattaa aggcagcttc tgcgatggag taatcggtgt ctgcattcat
     9961 gtgtttgttt ttaatcaggt caaccaggaa ggtcagaccc gctttcgcgc cagcgttatc
    10021 cacgcccacg tctttaatgt cgtatttgcc attttcatac ttgaacgcat aacccccgtc
    10081 agcagcaatc agcggccagg tgaagtacgg ttcttgcagg ttgaacatca gcgcgctctt
    10141 acctttcgct ttcagttctt tatccagcgc cgggatctct tcccaggttt ttggtgggtt
    10201 cggcagcaga tctttgttat aaatcagcga taacgcttca acagcgatcg ggtaagcaat
    10261 cagcttgccg ttgtaacgta cggcatccca ggtaaacgga tacagcttgt cctggaacgc
    10321 tttgtccggg gtgatttcag ccaacaggcc agattgagcg tagccaccaa aacggtcgtg
    10381 cgcccagaag ataatgtcag ggccatcgcc agttgccgca acctgaggga atttctcttc
    10441 cagtttatcc ggatgctcaa cggtgacttt aattccggta tctttctcga atttcttacc
    10501 gacttcagcg agaccgttat agcctttatc gccgttaatc cagattacca gtttaccttc
    10561 ttcgattttg gcgagagccg aggcggaaaa catcatcgtc gttaatgcgg ataatgcgag
    10621 gatgcgtgca cctgttttta ttttcataat ctatggtcct tgttggtgaa gtgctcgtga
    10681 aaacacctaa acggactcta gtttctttat acggcaacct ctttccatcc tccttgcccc
    10741 tacgccccac cgttgctttg tgtgatctct gttacagaat tggcggtaat gtggagatgc
    10801 gcacataaaa tcgccacgat ttttgcaagc aacatcacga aattccttac atgacctcgg
    10861 tttagttcac agaagccgtg ttctcatcct cccgcctcct cccccataaa aaagccaggg
    10921 ggtggaggat ttaagccatc tcctgatgac gcatagtcag cccatcatga atgttgctgt
    10981 cgatgacagg ttgttacaaa gggagaaggg catggcgagc gtacagctgc aaaatgtaac
    11041 gaaagcctgg ggcgaggtcg tggtatcgaa agatatcaat ctcgatatcc atgaaggtga
    11101 attcgtggtg tttgtcggac cgtctggctg cggtaaatcg actttactgc gcatgattgc
    11161 cgggcttgag acgatcacca gcggcgacct gttcatcggt gagaaacgga tgaatgacac
    11221 tccgccagca gaacgcggcg ttggtatggt gtttcagtct tacgcgctct atccccacct
    11281 gtcagtagca gaaaacatgt catttggcct gaaactggct ggcgcaaaaa aagaggtgat
    11341 taaccaacgc gttaaccagg tggcggaagt gctgcaactg gcgcatttgc tggatcgcaa
    11401 accgaaagcg ctctctggcg gtcagcgtca gcgtgtggcg attggccgta cgctggtggc
    11461 cgagccaagc gtatttttgc tcgatgaacc gctctccaac ctcgatgctg cactgcgtgt
    11521 gcaaatgcgt atcgaaatct cccgtctgca taaacgcctg ggccgcacaa tgatttacgt
    11581 cacccacgat caggtcgaag cgatgacgct ggccgacaaa atcgtggtgc tggacgcagg
    11641 tcgcgtggcg caggttggga aaccgctgga gctgtaccac tatccggcag accgttttgt
    11701 cgccggattt atcggttcgc caaagatgaa cttcctgccg gtaaaagtga ccgccaccgc
    11761 aatcgatcaa gtgcaggtgg agctgccgat gccaaatcgt cagcaagtct ggctgccagt
    11821 tgaaagccgt gatgtccagg ttggagccaa tatgtcgctg ggtattcgcc cggaacatct
    11881 actgccgagt gatatcgctg acgtcatcct tgagggtgaa gttcaggtcg tcgagcaact
    11941 cggcaacgaa acccaaatcc atatccagat cccttccatt cgtcaaaacc tggtgtaccg
    12001 ccagaacgac gtggtgttgg tagaagaagg tgccacattc gctatcggcc tgccgccaga
    12061 gcgttgccat ctgttccgtg aggatggcac tgcatgtcgt cgactgcata aggagccggg
    12121 cgtttaagca ccccacaaaa cacacaaagc ctgtcacagg tgatgtgaaa aaagaaaagc
    12181 aatgactcag gagatagaat gatgattact ctgcgcaaac ttcctctggc ggttgccgtc
    12241 gcagcgggcg taatgtctgc tcaggcaatg gctgttgatt tccacggcta tgcacgttcc
    12301 ggtattggct ggacaggtag cggcggtgaa caacagtgtt tccagactac cggtgctcaa
    12361 agtaaatacc gtcttggcaa cgaatgtgaa acttatgctg aattaaaatt gggtcaggaa
    12421 gtgtggaaag agggcgataa gagcttctat ttcgacacta acgtggccta ttccgtcgcg
    12481 caacagaatg actgggaagc taccgatccg gccttccgtg aagcaaacgt gcagggtaaa
    12541 aacctgatcg aatggctgcc aggttccacc atctgggcag gtaagcgctt ctaccaacgt
    12601 catgacgttc atatgatcga cttctactac tgggatattt ctggtcctgg tgccggtctg
    12661 gaaaacatcg atgttggctt cggtaaactc tctctggcag caacccgctc ctctgaagct
    12721 ggtggttctt cctcttttgc cagcaacaat atttatgact ataccaacga aaccgcgaac
    12781 gacgttttcg atgtgcgttt agcgcagatg gaaatcaacc cgggcggcac attagaactg
    12841 ggtgtcgact acggtcgtgc caacctgcgt gataactatc gtctggttga tggcgcatcg
    12901 aaagacggct ggttattcac tgctgaacat actcagagtg tcctgaaggg ctttaacaag
    12961 tttgttgttc agtacgctac tgactcgatg acctcgcagg gtaaaggtct gtcgcagggt
    13021 tctggcgtcg cgtttgataa cgaaaaattt gcctacaata tcaacaacaa cggtcacatg
    13081 ctgcgtatcc tcgaccacgg tgcgatctcc atgggcgaca actgggacat gatgtacgtg
    13141 ggtatgtacc aggatatcaa ctgggataac gacaacggca ccaagtggtg gaccgttggt
    13201 attcgcccga tgtacaagtg gacgccaatc atgagcaccg tgatggaaat cggctacgac
    13261 aacgtcgaat cccagcgcac cggcgacaag aacaatcagt acaaaattac cctcgcacaa
    13321 caatggcagg ctggcgacag catctggtca cgcccggcta ttcgtgtctt cgcaacctac
    13381 gccaagtggg atgagaaatg gggttatgac tacaacggcg atagcaaggt taacccgaac
    13441 tacggcaaag ccgttcctgc tgatttcaac ggcggcagct tcggtcgtgg cgacagcgac
    13501 gagtggacct tcggtgccca gatggaaatc tggtggtaat agcaaaacct gggccggata
    13561 aggcgtttac gccgcattcg gcaaccaacg cctgatgcga cgcttgcgcg tcttatcagg
    13621 ccgacaacgg ctgtcaaatg taggccggat aaggcgttta cgccgcatcc ggcataaaaa
    13681 caggttgtca ttatctgaaa ggggcgaaag cccctctgat tatcgggttt agcgcgctat
    13741 tgcctggcta ccgctgagct ccagattttg aggtgaaaac aatgaaaatg aataaaagtc
    13801 tcatcgccct ctgtttatca gcagggttac tggcaagcgc gcctggaatc agccttgccg
    13861 atgttaacta cgtaccgcaa aacaccagcg acgcgccagc cattccatct gctgcgctgc
    13921 aacaactcac ctggacaccg gtcgatcaat ctaaaaccca gaccacccaa ctggcgaccg
    13981 gcggccaaca actgaacgtt cccggcatca gtggtccggt tgctgcgtac agcgtcccgg
    14041 caaacattgg cgaactgacc ctgacgctga ccagcgaagt gaacaaacaa accagcgttt
    14101 ttgcgccgaa cgtgctgatt cttgatcaga acatgacccc atcagccttc ttccccagca
    14161 gttatttcac ctaccaggaa ccaggcgtga tgagtgcaga tcggctggaa ggcgttatgc
    14221 gcctgacacc ggcgttgggg cagcaaaaac tttatgttct ggtctttacc acggaaaaag
    14281 atctccagca gacgacccaa ctgctcgacc cggctaaagc ctatgccaag ggcgtcggta
    14341 actcgatccc ggatatcccc gatccggttg ctcgtcatac caccgatggc ttactgaaac
    14401 tgaaagtgaa aacgaactcc agctccagcg tgttggtagg acctttattt ggttcttccg
    14461 ctccagctcc ggttacggta ggtaacacgg cggcaccagc tgtggctgca cccgctccgg
    14521 caccggtgaa gaaaagcgag ccgatgctca acgacacgga aagttatttt aataccgcga
    14581 tcaaaaacgc tgtcgcgaaa ggtgatgttg ataaggcgtt aaaactgctt gatgaagctg
    14641 aacgcctggg atcgacatct gcccgttcca cctttatcag cagtgtaaaa ggcaaggggt
    14701 aattacgccc cacagtgctg attttgcaac aactggtgcg tctcctggcg cacctttttt
    14761 tatgcttcct tcctgggata tgagcgattt tttatagtaa ctcacttctt cttcactaag
    14821 aatatccatt atctcaatgc cttatcagag attcttttcc tttcgccggt agtgtctgga
    14881 cattcaggct acttttccag gttattttat ttctgttatg cagaggtttt atgataagtc
    14941 atatcctaaa ttctggcggc aataactctt tgatgaaaca tgatgtggtg caaggaaata
    15001 atatagtaga tcttgattta ctacgtaatt tcaatggggt gccaggttta aacagagata
    15061 actttattta tatcagcaat attttttcaa atataaaaca acggaacgaa aaaatcatgc
    15121 aataaatttg tttcgtgaag tctcaatcag taatgatact ataagtgtaa aattctacag
    15181 aaatgaaaaa aattgaatgc gcttgcaatt ttctgatgga taaagatgcg caggggtata
    15241 tcgacctgtc tgatttggat ttaacaagtt gtcattttaa aggtgacgtt atatcgaagg
    15301 tgtctttttt atcatcaaat ctacaacatg taacattcga atgtaaagaa attggggatt
    15361 gcaattttac tactgcaata gttgataatg tcatatttag atgtcgacgt ttacacaatg
    15421 tgatttttat caaagcgagt ggtgaatgtg tcgatttcag caaaaatatt cttgatacag
    15481 ttgacttctc gcagagtcaa cttactcaga gtaattttcg cgaatgtcag attagaaatt
    15541 caaacttcga taattgttat ctttacgctt cgcacttcac cagagcagag tttctgtctg
    15601 ccaaagaagt atcatttatt aaatcgaata tgacagctgt tatgtttgat catgtgcgaa
    15661 tatcgacagg gaattttaaa gattgcatta cagaacaatt ggaattaact attgattatt
    15721 cagatat
//
LOCUS       DAZFWL020000108        15504 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFWL020000108 DAZFWL020000000
VERSION     DAZFWL020000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.108"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            31..597
                     /gene="yceJ"
                     /locus_tag="ACHKNP_RS18010"
                     /old_locus_tag="pgaptmp_003602"
     CDS             31..597
                     /gene="yceJ"
                     /locus_tag="ACHKNP_RS18010"
                     /old_locus_tag="pgaptmp_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU4705698.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            601..1176
                     /gene="yceI"
                     /locus_tag="ACHKNP_RS18015"
                     /old_locus_tag="pgaptmp_003603"
     CDS             601..1176
                     /gene="yceI"
                     /locus_tag="ACHKNP_RS18015"
                     /old_locus_tag="pgaptmp_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HGU4705699.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1218..2270)
                     /gene="trhO"
                     /locus_tag="ACHKNP_RS18020"
                     /old_locus_tag="pgaptmp_003604"
     CDS             complement(1218..2270)
                     /gene="trhO"
                     /locus_tag="ACHKNP_RS18020"
                     /old_locus_tag="pgaptmp_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGU4705700.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2495..3415
                     /gene="lpxL"
                     /locus_tag="ACHKNP_RS18025"
                     /old_locus_tag="pgaptmp_003605"
     CDS             2495..3415
                     /gene="lpxL"
                     /locus_tag="ACHKNP_RS18025"
                     /old_locus_tag="pgaptmp_003605"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HGU4705701.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPQQYMWLHRRFKTRPEGVPSRY"
     gene            3587..4813
                     /gene="mdtG"
                     /locus_tag="ACHKNP_RS18030"
                     /old_locus_tag="pgaptmp_003606"
     CDS             3587..4813
                     /gene="mdtG"
                     /locus_tag="ACHKNP_RS18030"
                     /old_locus_tag="pgaptmp_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HGU4705702.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4896..5270
                     /gene="msyB"
                     /locus_tag="ACHKNP_RS18035"
                     /old_locus_tag="pgaptmp_003607"
     CDS             4896..5270
                     /gene="msyB"
                     /locus_tag="ACHKNP_RS18035"
                     /old_locus_tag="pgaptmp_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU4705703.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5271..5498)
                     /gene="yceK"
                     /locus_tag="ACHKNP_RS18040"
                     /old_locus_tag="pgaptmp_003608"
     CDS             complement(5271..5498)
                     /gene="yceK"
                     /locus_tag="ACHKNP_RS18040"
                     /old_locus_tag="pgaptmp_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU4705704.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5671..8214)
                     /gene="mdoH"
                     /locus_tag="ACHKNP_RS18045"
                     /old_locus_tag="pgaptmp_003609"
     CDS             complement(5671..8214)
                     /gene="mdoH"
                     /locus_tag="ACHKNP_RS18045"
                     /old_locus_tag="pgaptmp_003609"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HGU4705705.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8207..9742)
                     /gene="mdoG"
                     /locus_tag="ACHKNP_RS18050"
                     /old_locus_tag="pgaptmp_003610"
     CDS             complement(8207..9742)
                     /gene="mdoG"
                     /locus_tag="ACHKNP_RS18050"
                     /old_locus_tag="pgaptmp_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HGU4705706.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10136..11293
                     /gene="mdoC"
                     /locus_tag="ACHKNP_RS18055"
                     /old_locus_tag="pgaptmp_003611"
     CDS             10136..11293
                     /gene="mdoC"
                     /locus_tag="ACHKNP_RS18055"
                     /old_locus_tag="pgaptmp_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HGU4705707.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11301..12782)
                     /gene="clsC"
                     /locus_tag="ACHKNP_RS18060"
                     /old_locus_tag="pgaptmp_003612"
     CDS             complement(11301..12782)
                     /gene="clsC"
                     /locus_tag="ACHKNP_RS18060"
                     /old_locus_tag="pgaptmp_003612"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HGU4705708.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12724..13257)
                     /gene="ymdB"
                     /locus_tag="ACHKNP_RS18065"
                     /old_locus_tag="pgaptmp_003613"
     CDS             complement(12724..13257)
                     /gene="ymdB"
                     /locus_tag="ACHKNP_RS18065"
                     /old_locus_tag="pgaptmp_003613"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HGU4705709.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13352..13663)
                     /gene="ymdA"
                     /locus_tag="ACHKNP_RS18070"
                     /old_locus_tag="pgaptmp_003614"
     CDS             complement(13352..13663)
                     /gene="ymdA"
                     /locus_tag="ACHKNP_RS18070"
                     /old_locus_tag="pgaptmp_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HGU4705710.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13784..14116)
                     /gene="csgC"
                     /locus_tag="ACHKNP_RS18075"
                     /old_locus_tag="pgaptmp_003615"
     CDS             complement(13784..14116)
                     /gene="csgC"
                     /locus_tag="ACHKNP_RS18075"
                     /old_locus_tag="pgaptmp_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HGU4705711.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14175..14630)
                     /gene="csgA"
                     /locus_tag="ACHKNP_RS18080"
                     /old_locus_tag="pgaptmp_003616"
     CDS             complement(14175..14630)
                     /gene="csgA"
                     /locus_tag="ACHKNP_RS18080"
                     /old_locus_tag="pgaptmp_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HGU4705712.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14671..15126)
                     /gene="csgB"
                     /locus_tag="ACHKNP_RS18085"
                     /old_locus_tag="pgaptmp_003617"
     CDS             complement(14671..15126)
                     /gene="csgB"
                     /locus_tag="ACHKNP_RS18085"
                     /old_locus_tag="pgaptmp_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HGU4705713.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            complement(15255..>15504)
                     /locus_tag="ACHKNP_RS18090"
                     /old_locus_tag="pgaptmp_003618"
     CDS             complement(15255..>15504)
                     /locus_tag="ACHKNP_RS18090"
                     /old_locus_tag="pgaptmp_003618"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705714.1"
                     /translation="SCFFSAPPWTIFYCKKTRFVTACAQDISQQSATGKKNDCLVIFD
                     SGKRRFKRKQNIFCVDNSVFTWVLILWYEQKKKNTTRG"
BASE COUNT         3855 a         3983 c         3689 g         3977 t
ORIGIN      
        1 ttctctcgca aattcgtatt ggatgcactt atgtcattca caaatacccc tgaacgctat
       61 ggtgttatat ctgcggcctt ccattggcta agcgcaatta ttgtctacgg catgtttgct
      121 ttaggtctgt ggatggtcac gctcagttat tacgatggct ggtatcacaa agcacccgaa
      181 ctgcataaaa gcatcggtat tttgttaatg atggggctgg ttattcgcgt tctgtggcgt
      241 gtcatatctc caccgccggg tccgctgccg agttattcgc ccatgactcg tcttgccgca
      301 aaggctggac atctcgcatt gtacctattg ctgttcgcca tcggcatcag cggctatctg
      361 atctcaactg ccgatggtaa accgatcagc gtttttggct ggtttgacgt ccccgcgacc
      421 cttgccgacg ctggcgcaca ggcagacttt gccggtgccc tgcatttttg gctcgcgtgg
      481 agcgtcgtag tactgtccgt tatgcacgga tttatggccc tgaagcatca tttcatcgat
      541 aaagacgaca ctctgaagcg catgctggga aagtcgtcat ctgactatgg agtataaaaa
      601 atgaaaaaaa gcctgcttgg tttaaccttc gcgtccctga tgttctctgc cggttcagcg
      661 gttgccgccg attacaaaat tgacaaagaa ggtcagcacg cctttgttaa cttccgcatc
      721 cagcatcttg gctatagctg gttatatggc acctttaaag atttcgacgg tacttttacc
      781 tttgacgaaa aaaatccggc tgccgataaa gtgaatgtga caattaacac caccagcgtc
      841 gatactaatc acgccgaacg cgataaacat cttcgcagtg cagatttcct caataccgca
      901 aaatatccac aggcaacatt cacctccacc agcgtgaaga aagacggtga cgaactggat
      961 attaccggcg atctgacgct gaatggcgta accaaacccg tcacgctgga agcgaaatta
     1021 attggtcagg gcgacgaccc atggggtggt aaacgtgcag gcttcgaggc cgaaggcaaa
     1081 attaagctca aggacttcaa tatcaagaca gatttaggtc cagcttctca ggaagtggat
     1141 ctgattattt cagtggaagg cgtacagcag aagtaagcgt gaaatgttgc cggatggcac
     1201 gcatccggca atgatattta ttccgttgga tcaggaatgc ccagtgttgt attcagacgt
     1261 ccacgagact tattaaagat cttattgcca ttttcacgtc ctgcccgacg gcgtcgctgt
     1321 tcttctggcg gtaacgcgct ttcttcgcag caaatctcac tacaacaacc tttgtatttt
     1381 tccgcacata ctggacactg aataaacagc aggtggcagc catcattttt acagttggta
     1441 tggctgtcgc acggcgcgcc gcactggtgg caatgcgcga taatctcatc cgatatacgt
     1501 tcgcccatcc gctcgtcaaa aacaaaattt ttgccaataa aacgcaccgg caagccctgc
     1561 tcgcgcgcct tacgggcgta ttcaataatt ccgccctcga tatgccagac tttgttgaat
     1621 ccgttatgtt tcatccaggc actggccttt tcacaacgaa tgccgccggt gcagtacatg
     1681 acgatttttt tatctttatg tgcctgcatc atctcgactg cttttggcag ctgctcacgg
     1741 aaggtatctg ccggaatttc gagcgcgttt tcaaagtgcc ccacttcata ctcatagtgg
     1801 ttacgcatgt cgataaatag tgcatcggga tcgtcaagca tggcgttcac ttccgccgct
     1861 tgcagatact cgccaacatt gctggcatca aagtgaggat cgtcaatacc gtcggcaaca
     1921 atgcgatcgc gtaccttcat gcgcagtacc cagaaagatt tcccgtcatc atccaacgcg
     1981 atattcaggc gtaaaccctc taaagccggg tcgaaggcat agagctgcgc gcgaaatgtt
     2041 tcaacattgc tcgcaggtac gctgatttgc gcgttaatgc cctcatgcgc cagatacact
     2101 cgcccaaaaa cattcagcgc ggtaaacagc tgatataaag cgtcacgggt cgccttagga
     2161 tcggcgatgt ggaaatactt gtaaaacgaa atggtggttc gcggttcgct ctcagccaac
     2221 attttggctt ttagcgcgtc gttggaaatg cggttgtgta acactggcat ggtgtacggt
     2281 tcctgcgaga tgggaaagta aaaatccgcg gcatgatata gcaattatcg ataattaaca
     2341 tccacacatt ttacgctaca tttgcgcatt aaaaattatt tgttatttac aagtgcggca
     2401 atttcgccca gtcttcagcc acaaattttg gttgcgggcg aaaaaatgcg acaatacata
     2461 caattgcccg aataggttga aaaacaggat tgatatgacg aatctaccca agttctccac
     2521 cgcactgctt catccgcgtt attggttaac ctggttgggt attggcgtac tttggttagt
     2581 cgtgcaattg ccctacccgg ttatctaccg cctcggttgt ggattaggaa aactggcgtt
     2641 acgttttatg aaacgacgcg caaaaattgt gcatcgcaac ctggaactgt gcttcccgga
     2701 aatgagcgaa caagaacgcc gtaaaatggt ggtgaagaat ttcgaatccg ttggcatggg
     2761 cctgatggaa accggcatgg cgtggttctg gccggaccgc cgaatcgccc gctggacgga
     2821 agtgatcggc atggaacaca ttcgtgacgt gcaggcgcaa aaacgcggca tcctgttagt
     2881 tggcatccat tttctgacac tggagctggg tgcgcggcag tttggtatgc aggaaccggg
     2941 gattggcgtt taccgcccga acgataatcc actgattgac tggctacaaa cctggggccg
     3001 tttgcgctca aataaatcga tgctcgaccg caaagattta aaaggcatga ttaaagccct
     3061 gaaaaaaggc gaagtggtct ggtacgcacc ggatcatgat tacggcccgc gctcaagcgt
     3121 tttcgtcccg ttatttgccg ttgagcaggc tgcgaccacg accggaacct ggatgctggc
     3181 acggatgtcc ggcgcatgtc tggtgccctt cgttccacgc cgtaagccag atggcaaagg
     3241 gtatcaattg attatgctgc cgccagagtg ttctccgcca ctggatgatg ccgaaactac
     3301 cgccgcgtgg atgaacaaag tggtcgaaaa atgcatcatg atggcaccac agcagtatat
     3361 gtggttacac cgtcgcttta aaacacgccc ggaaggcgtt ccttcacgct attaaatctc
     3421 ccctgccgga tgcttcagaa tggcatccgg cattaccaca gcaaatcccc ctgatttagc
     3481 gataaaagct ctctggattg cgccccctgg aagtcgagcg cataattagt gtgcttatct
     3541 tttcttctta tgttcaccgc gcctggcgca ccaacagcgg attgctatgt caccctgtga
     3601 aaatgacacc cctataaact ggaaacgaaa cctgatcgtc gcctggctag gctgttttct
     3661 taccggtgcc gccttcagtc tggtaatgcc cttcttaccc ctctacgttg agcagcttgg
     3721 cgttaccggt cactccgccc tgaatatgtg gtccggtatt gtcttcagca ttacattttt
     3781 attttcggcc atcgcctcac cgttttgggg tggactcgcc gaccgtaaag gccgaaaact
     3841 catgctatta cgctctgccc tcggcatggg catcgtgatg gtgttgatgg gactggcaca
     3901 aaatatctgg cagtttttga tcctacgggc gcttcttggg ttacttggcg gatttgtccc
     3961 caacgctaat gctcttatcg ccacacaagt accgcgtaat aaaagcggct gggcgctggg
     4021 tacgctctcc acaggcggcg ttagtggtgc gttgctcggc ccaatggctg gcggcctgct
     4081 cgccgatagc tacggcttac gtccggtatt ctttattacc gccagtgtgc tcatactctg
     4141 ctttttcgtc accctgttct gcatcagaga aaaattccag ccggtcagca aaaaagagat
     4201 gctgcacatg cgggaagtgg tgacatcact taaaaacccg aaactggtac tcagcctgtt
     4261 tgtcaccacg ttaatcatcc aggtggcgac aggctcaatt gcccccattc tgacgctgta
     4321 tgtccgcgaa ctggcgggta acgtcagtaa cgtcgccttt atcagtggca tgatcgcctc
     4381 ggtgccaggc gtggcggctc tgctaagcgc accacgactc ggcaaacttg gcgatcgaat
     4441 cggacccgaa aagatcctga ttacagcgct gatcttttct gtactgctgt tgatcccaat
     4501 gtcttacgtt cagacgccat tgcaacttgg gattttacgt tttttgctcg gtgccgccga
     4561 tggtgcacta ctccccgccg tacagacact gttggtttac aactcgagca accagatcgc
     4621 cgggcgtatc ttcagctata accaatcgtt tcgtgatatt ggcaacgtta ccggaccatt
     4681 gatgggagca gcgatttcag cgaactacgg tttcagagcg gtatttctcg tcaccgctgg
     4741 cgtagtgtta ttcaacgcag tctattcatg gaacagtcta cgtcgtcgtc gaatacccca
     4801 ggtatcgaac tgatttttcg cctttcatac ttgcaaaagc ggagaatcag ctatcctttt
     4861 ccctgaaacc tcatcaactc aaagggagaa tcgtgatgac catgtacgca acgcttgaag
     4921 aagccattga cgctgcacgc gaagaatttc ttgcagacaa ccccggcatc gacgccgaag
     4981 atgcgaatgt gcaacagttc aatgcccaaa aatacgtttt gcaggacggc gacatcatgt
     5041 ggcaagttga gttttttgcc gacgaagggg aagaaggtga atgtttacct atgcttagcg
     5101 gtgaagccgc gcaaagtgtt tttgatggcg actatgatga gatagagata cgccaggagt
     5161 ggcaggaaga gaatacatta catgaatggg acgaggggga atttcagctt gagccaccgc
     5221 tggataccga ggaaggacgc gcagcagctg atgagtggga tgaacgttaa tcactcatac
     5281 gggccatgat gaatgtcgat cggcagcagt aaagtatcaa aaaccagaga gaatggcaga
     5341 tcaaggatcg tgacataacg ccaggcggag tcacgcacat cccattgcac accgggataa
     5401 tattggttgc catgcccctg ccccggtata gtgcgactaa taatgctgcc acagccactc
     5461 agcaaggtca ccatgatgct caccacaatt aaacgcattt gatacctctt tcgtaaaaaa
     5521 ataccggcat aacgccggta tttttattgg ctcgttgttt ttgccggatg cggcgtgaac
     5581 gccttatccg acccacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg
     5641 ataagcgtag cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa
     5701 tgccagtgga ttgagcttta tcccttcgta ataactcacc catgaagaat atctctccgg
     5761 ggaattccag acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag
     5821 gcgacgatcg cgattcagct tctctggcgt ctcgttcagc gcctgttcaa cgtggcggtc
     5881 acgggcgatt tccagcacct tactggcgcg gtgacgcgcg gtcgccattg cggttgccag
     5941 agcgttaaat gacgggttaa acactgcgtg cataaagcca tcatcaaggg agcgttgacg
     6001 attcatctca aggaaccgat cggtatcaac cagcacctgc ggcggcgaat actcttccgg
     6061 gatcaggaac agtttccagc gtttggtgcg cagaccaacg gtggcacggc tggaaatcac
     6121 cgaaacaaac ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg
     6181 cagatccaac cacgccatac caacagccca cactaaccct aacagcagct gtgagccgtg
     6241 gcgtttgaac gcttcacccc aggaagtgga gtcatcatca cgctgcggtg aattccacac
     6301 cacttcccag ccaaggaacg cgctgacgac gaagaccgta tggaacagca tgcgtaccgg
     6361 agccagcagc acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc
     6421 gccgtattct ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa
     6481 caacagcacc atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca
     6541 caccgggaac aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgtactac
     6601 ctgcaatgca gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat
     6661 cacgcccgtc aggaataccg cacggtgaac cgggtgcata cccttcacca ggaacagacg
     6721 gaagttcatc aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt
     6781 aggcggcaat tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc
     6841 acggcgcatc aacgccgctt ccacgaagtc atgtgacagg attgaaccgg caaaggaacc
     6901 ttcgcccggc agcggagcca gtgcgcagtg ctcgataaac ggtttcacgc ggataatcgc
     6961 gttatgtccc cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag
     7021 tggcccatac acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga
     7081 cgctttcggc gacgactgaa tgatcccggc gttcgggttg gcttccatca ggcgcaccag
     7141 gccgcataaa caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta
     7201 ctggctgccc cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg
     7261 acggcggcgg cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca
     7321 ggctttttgc tctgcgacgc agatatccgg gttataactg tcactaagaa tgtagacatc
     7381 aaagtgtttg gcattcccgg tggcttttac tgattcccac gttgcacgca ggccagcaaa
     7441 aacacggttc acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt
     7501 taatggttca tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag
     7561 ttgcaggaag cccattaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc
     7621 aaagaggatc aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca
     7681 caaatcctga ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat
     7741 ggtcttcata taccaggtcg cgacgacagt ttgcgcgagc gtcaggatca acagaatgta
     7801 acggcggatg gtaccgacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa
     7861 acgagccaga tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac
     7921 cgggttggta cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt
     7981 cagctgatcg cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca
     8041 ggcttgttcc agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt
     8101 gcggtgttcg gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgccgc
     8161 tttctcgctt gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag
     8221 gtaactggta gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca
     8281 tttcagtggt tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg
     8341 taaccgggtt atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt
     8401 gcgctgtgac cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca
     8461 caaaggcgat agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg
     8521 aacgacgcgt ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc
     8581 tgaaggtgat ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg
     8641 tccagtaagc gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa
     8701 cgctgccttt accccactcc cctttcggag tcacccatgc gcttggacga agatcgtaac
     8761 gatcatcgag atcttcaaag cgggagaaat cacgaccgcg ctgcaacaga ccgaagcctt
     8821 gcgggttctc catcgagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc
     8881 agatccattc gccattaccg gcatggatag acagaccgtt agagtcgtgc aactccggac
     8941 gatagttatt tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg
     9001 caacccccag tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg
     9061 tgtcacgccc tggcataacc acgaatttgt aagcacctgt cgcgcgcggc gagtcaagca
     9121 atgcataaat ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga
     9181 agcgtggaaa ttcttcaccc gatggcaagg cggtatcaat tgccaggccg cgggcagaaa
     9241 ggccataaac ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga
     9301 tttcatcgtt tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa
     9361 ggtcttttac cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt
     9421 atttgattcg tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt
     9481 acataccctg atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt
     9541 tgtcatgatt aaactggatc tgctgatagt ccgcgtattt catatcgcgg aaaacggagg
     9601 gcaagttgct tttgggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga
     9661 catcatcaat actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac
     9721 tcaaccaacg cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc
     9781 tttgtgtgct tatatcgatc cgatctattt taatggataa ccagccattc cgacaacaga
     9841 atcccggtat cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca
     9901 ctttccgtgc ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaattt
     9961 tatttatcaa ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc
    10021 ataaaaacct tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc
    10081 taaaggcata acccgacagg gataagacga aaaatccgag attagttaac catatatgaa
    10141 tccagtaccc gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt
    10201 agggatccct tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc
    10261 cgaaccatca ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgcaggt
    10321 atttttcgtt atatccggct acttttccta catgcttttt ttacgctatc ccttgaaaaa
    10381 atggtggaaa gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccctact
    10441 gacattaccg caatttatta tgctgcaata tgtcaaagga aaagcggaaa gttggcctgg
    10501 gctgtcattg tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt
    10561 tttactggtg ttagtagtca tgacgacgct gtgcgtatgg atatttaagc gcatcagaaa
    10621 taatttagaa aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat
    10681 ttttttatgc ctcggcatcg gttatgcggt aataagaaga acgattttta ttgtgtatcc
    10741 gcccattctg agtaatggca tgttcaattt tattgtcatg caaacgctgt tttatttgcc
    10801 gttctttatc ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac
    10861 gccgtctcgt ggctgtaccc ttgcagcagc attggcgttt gtcgcttatt tactcaacca
    10921 gcgctatggc agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct
    10981 cggtctgtgg atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc
    11041 agcgcgggtg acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt
    11101 aacgctgttt ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct
    11161 ctgtggcctg atatttgtag tagggattgc gataattctg tatgaaattc atttgcgcat
    11221 cccgttactg aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc
    11281 accagcccgt taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg
    11341 accataaccc gcttccagaa actggtggcg ggttcttttt tgagaataat ctctttcttt
    11401 gcatgacgat caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca
    11461 tcatactggc tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc
    11521 acgaagccca tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata
    11581 aacaccgttt taccatcgat gctaaaggtt ttagcatgca ggctggctcc ggaattaccg
    11641 gttatgccgc gatcgtgtaa cgtactactt tgttcacgcg tcggcttgag ttcatataat
    11701 tccacgccat agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca
    11761 acatcgttag cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc
    11821 cgtaagagtt gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg
    11881 cgttcactgg gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt
    11941 gccttgccct cccctttcgc cggatcatca cttaataaac gtgttttcgc ccagataagc
    12001 ggcaacgttc catcaaccag atgatttata aacggactgg attccatttt gcgtaagtaa
    12061 cgatgcgtca tagcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca
    12121 ccttccggga catccagcac ttgctgtaag ggtgaaaccg atttgcaata ccagtagcgg
    12181 gcgaaatcat cggcaacgtc ctctaccacg ggccctattg ccataacatc taaatccgaa
    12241 aaaagtggct cttcccctgc cccaaaatag gcatcaccaa tatttcgccc ccctaccagc
    12301 gtcaccacgc catcgacagt gaaactttta ttgtgcatac ggcgattgag acgggaaaag
    12361 tcggtgatat aaccaagcgg acgcagcaag cgaaacgaga aaggattaaa aagccggact
    12421 tcaatgcggg gatggctgtc aagcaagcgt aaaatgtcgt caagtccggg cgtattgttg
    12481 tcgtccagca acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac
    12541 agtaaccgac ccgacatatc gtcctgccag atgtaatact gaacatccag cgtatgctct
    12601 gccatttcgg ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa
    12661 agaccacact gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc
    12721 aaatcattca tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc
    12781 ataacagaca aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt
    12841 tttaactgcg atttcagccg ctgccgcacg agggtaacca taaaccccag tactgattgc
    12901 aggaaaagcc actgacgtat agctgtttgc cgccaccagt cgtaggctat tgagataggc
    12961 atcctgcaaa agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg
    13021 cactacggct ttagcgggaa gagcgcctgc aagcgtaata acggcatgcc ccgtagggca
    13081 atcgccctgc tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg
    13141 atgaatggcc ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat
    13201 cacatcaacg gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac
    13261 gcctccttac accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt
    13321 ttctgctcag caacgtgcgt tgttcagaaa cttagttata ttcgatgttc attacagcca
    13381 gctttttctg tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttaacat
    13441 taccgctcat taacgctttg ctggaatagg ttttgcccgg aacagaacca ttctgtgggc
    13501 agttcatttc tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc
    13561 cataaaaatg gatcaccccc ccttgcgcgg tgcttcctgc gatggcaata aaaggaaaaa
    13621 gcaaactgcc gagcataaga gagtttaaaa aaggtctgaa catttattct atttcctcaa
    13681 tgattagtca tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag
    13741 accattctct ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag
    13801 agggcggcca ttgttgtgat aaatgaagtg actgtccatc agaaacagta acaactattt
    13861 tcacccgatc ttccggggaa atatttaaac tcaacttcgt caaagcaata ggttgattag
    13921 caggcaatga gagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca
    13981 gggacaatat ttgtactctg cacagacaag attgagtaag agtgacttca ggaataatgg
    14041 tatacacatc cccttgctgg gtcgtattaa aggttatctg gctggaaagt gccgcaagga
    14101 gtaataacgc attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta
    14161 atacaaatga tgtattagta ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc
    14221 acgttgacgg aggagttaga tgcagtctgg tcaactgcag caccgttgcc accaccgaac
    14281 tgtttaacag tcatttcaga attttttccg ttccactgat caagagtggc gctgttaccg
    14341 aagccacgtt gggtcagatc gattgagctg tcatctgagc cctgacctac atctgcacca
    14401 ttaccaccgc catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca
    14461 agtgcagagt taccgccacc gtactggtaa atgttcagct ctgaatttgg gccgctatta
    14521 ttaccgccac caccgtggtt accgccgccg ccgtactgag gaacaacacc tgccagagcg
    14581 ctaccggaga atacgattgc tgcaattgct gctactttta aaagtttcat gtaaaacccc
    14641 catcggattg atttaaaagt cgaatggaaa ttaacgttgt gtcacgcgaa tagccatttg
    14701 cgactgtctc tgcactacaa ttgccgtttt ttgagtacca tactgtgtaa tatttgcttt
    14761 attaccagaa cctttctgga taatcatcgc agtattacca taagcacctt gcgaaatact
    14821 ggcatcgttg gcactgcccg cctgatcaat atatgcaagg ttataatctc ctgtctggtc
    14881 aatctttgcc cggttgctac taccttcttg cgcaacaacc gccaaaagtt ttgagcctcc
    14941 ctgccgtaac tgagcactat tattcgtccc agcttgacca attatggctg cctgattaaa
    15001 tgaagactta ctcaattcat ttaccgcgaa gttatattct gaattagcta aatcataacc
    15061 tgctgcggct gcaatcccag gcgcacccag tattgttaac atcataaata acaatttgtt
    15121 tttcatgttg tcaccctgga cctggtcgta catttaagaa attaaatcat ttcaacttgg
    15181 ttgttaacgc aacctgtatt ttgttaacgc tgcgttacga tggaaagtat gtctgcggaa
    15241 atatttttaa taactcaccc gcgcgttgta tttttctttt tttgttcata ccaaagtatt
    15301 aaaacccacg taaatacgct gttatctacg caaaaaatat tttgttttct tttaaatctc
    15361 cgttttccgc tatcaaaaat caccagacag tcatttttct tgcccgtcgc tgattgctgc
    15421 gatatgtctt gcgcacaagc cgtgacaaac ctcgtctttt tgcagtaaaa aattgtccac
    15481 ggaggtgcgg agaaaaaaca agaa
//
LOCUS       DAZFWL020000109        15481 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFWL020000109 DAZFWL020000000
VERSION     DAZFWL020000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.109"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..128
                     /locus_tag="ACHKNP_RS18095"
                     /old_locus_tag="pgaptmp_003619"
     CDS             <1..128
                     /locus_tag="ACHKNP_RS18095"
                     /old_locus_tag="pgaptmp_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU4705715.1"
                     /translation="FNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKICPYGLK"
     gene            complement(231..1481)
                     /gene="icd"
                     /locus_tag="ACHKNP_RS18100"
                     /old_locus_tag="pgaptmp_003620"
     CDS             complement(231..1481)
                     /gene="icd"
                     /locus_tag="ACHKNP_RS18100"
                     /old_locus_tag="pgaptmp_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HGU4705716.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1653..2306
                     /gene="rluE"
                     /locus_tag="ACHKNP_RS18105"
                     /old_locus_tag="pgaptmp_003621"
     CDS             1653..2306
                     /gene="rluE"
                     /locus_tag="ACHKNP_RS18105"
                     /old_locus_tag="pgaptmp_003621"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HGU4705717.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2316..2777
                     /gene="nudJ"
                     /locus_tag="ACHKNP_RS18110"
                     /old_locus_tag="pgaptmp_003622"
     CDS             2316..2777
                     /gene="nudJ"
                     /locus_tag="ACHKNP_RS18110"
                     /old_locus_tag="pgaptmp_003622"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HGU4705718.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2831..3937
                     /gene="mnmA"
                     /locus_tag="ACHKNP_RS18115"
                     /old_locus_tag="pgaptmp_003623"
                     /gene_synonym="trmU"
     CDS             2831..3937
                     /gene="mnmA"
                     /locus_tag="ACHKNP_RS18115"
                     /old_locus_tag="pgaptmp_003623"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGU4705719.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3973..4614
                     /gene="hflD"
                     /locus_tag="ACHKNP_RS18120"
                     /old_locus_tag="pgaptmp_003624"
     CDS             3973..4614
                     /gene="hflD"
                     /locus_tag="ACHKNP_RS18120"
                     /old_locus_tag="pgaptmp_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HGU4705720.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4618..5988
                     /gene="purB"
                     /locus_tag="ACHKNP_RS18125"
                     /old_locus_tag="pgaptmp_003625"
     CDS             4618..5988
                     /gene="purB"
                     /locus_tag="ACHKNP_RS18125"
                     /old_locus_tag="pgaptmp_003625"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGU4705721.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6156..6827
                     /gene="phoP"
                     /locus_tag="ACHKNP_RS18130"
                     /old_locus_tag="pgaptmp_003626"
     CDS             6156..6827
                     /gene="phoP"
                     /locus_tag="ACHKNP_RS18130"
                     /old_locus_tag="pgaptmp_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HGU4705722.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6827..8287
                     /gene="phoQ"
                     /locus_tag="ACHKNP_RS18135"
                     /old_locus_tag="pgaptmp_003627"
     CDS             6827..8287
                     /gene="phoQ"
                     /locus_tag="ACHKNP_RS18135"
                     /old_locus_tag="pgaptmp_003627"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HGU4705723.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8363..9484
                     /gene="roxA"
                     /locus_tag="ACHKNP_RS18140"
                     /old_locus_tag="pgaptmp_003628"
                     /gene_synonym="ycfD"
     CDS             8363..9484
                     /gene="roxA"
                     /locus_tag="ACHKNP_RS18140"
                     /old_locus_tag="pgaptmp_003628"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HGU4705724.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9630..10859)
                     /gene="pepT"
                     /locus_tag="ACHKNP_RS18145"
                     /old_locus_tag="pgaptmp_003629"
     CDS             complement(9630..10859)
                     /gene="pepT"
                     /locus_tag="ACHKNP_RS18145"
                     /old_locus_tag="pgaptmp_003629"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGU4705725.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            11109..12245
                     /gene="potA"
                     /locus_tag="ACHKNP_RS18150"
                     /old_locus_tag="pgaptmp_003630"
     CDS             11109..12245
                     /gene="potA"
                     /locus_tag="ACHKNP_RS18150"
                     /old_locus_tag="pgaptmp_003630"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HGU4705726.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12229..13092
                     /gene="potB"
                     /locus_tag="ACHKNP_RS18155"
                     /old_locus_tag="pgaptmp_003631"
     CDS             12229..13092
                     /gene="potB"
                     /locus_tag="ACHKNP_RS18155"
                     /old_locus_tag="pgaptmp_003631"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HGU4705727.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVNELDD"
     gene            complement(13325..13474)
                     /locus_tag="ACHKNP_RS18160"
                     /old_locus_tag="pgaptmp_003632"
                     /pseudo
     CDS             complement(13325..13474)
                     /locus_tag="ACHKNP_RS18160"
                     /old_locus_tag="pgaptmp_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            13598..13683
                     /locus_tag="ACHKNP_RS18165"
                     /old_locus_tag="pgaptmp_003633"
                     /pseudo
     CDS             13598..13683
                     /locus_tag="ACHKNP_RS18165"
                     /old_locus_tag="pgaptmp_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(13706..14296)
                     /gene="espM2"
                     /locus_tag="ACHKNP_RS18170"
                     /old_locus_tag="pgaptmp_003634"
     CDS             complement(13706..14296)
                     /gene="espM2"
                     /locus_tag="ACHKNP_RS18170"
                     /old_locus_tag="pgaptmp_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HGU4705728.1"
                     /translation="MPMNTSGTSFSSFGISCHREGSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSRNILDTVNRQSAKDIDGWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFSLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPAYLTRAIQG"
     gene            14480..15130
                     /locus_tag="ACHKNP_RS18175"
                     /old_locus_tag="pgaptmp_003635"
     CDS             14480..15130
                     /locus_tag="ACHKNP_RS18175"
                     /old_locus_tag="pgaptmp_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU4705729.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPIPGGLWDHLGARESHHLADSLARQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
     gene            15205..>15481
                     /gene="espW"
                     /locus_tag="ACHKNP_RS18180"
                     /old_locus_tag="pgaptmp_003636"
     CDS             15205..>15481
                     /gene="espW"
                     /locus_tag="ACHKNP_RS18180"
                     /old_locus_tag="pgaptmp_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HGU4705730.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTNSVSRRIVHKEYGISL
                     KSVPVWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAINS"
BASE COUNT         3920 a         3841 c         3743 g         3977 t
ORIGIN      
        1 cattcaacaa ggatttatgg agtctactca aaagcagtca agagtgtcct ccaggagaaa
       61 gcaaaataac aaataaatgt ttgcataata gttgcattat aaaaatatgc ccatatgggc
      121 tcaagtaaag ggttttatat aaatatgctc gatatgctat taataatacc tacataaata
      181 aaacaacggg cgtgttatac gcccgttgca atattaaaca catgtagtga ttacatgttc
      241 ttgatgatcg catcaccaaa ttctgaacat ttcagcagtt tagcgccttc catcagacgc
      301 tcgaagtcat aggttacggt cttcgcattg attgcgcctt ccataccttt aacaatcagg
      361 tctgcggctt cagtccagcc catatggcgt aacatcatct cagcggagag aataatagag
      421 ccagggttta ctttgtcctg accggcgtat ttcggcgcag taccgtgggt ggcttcaaac
      481 agggcgcatt cgtcaccgat gtttgctcca ggggcgatac cgataccgcc aacctgcgct
      541 gccagagcgt cagaaatgta gtcaccgttc aggttcatac aggcgataac atcatattca
      601 gccggacgca gcaggatctg ttgcaggaat gcatcagcaa tcacgtcttt aatgacgatc
      661 tctttgccgg tgttcgggtt tttaactttc agccacgggc cgccgtcgat cagttcaccg
      721 ccaaactctt cacgcgccag ctggtagccc cagtctttaa acgcgccttc ggtgaacttc
      781 atgatgttgc ctttgtgcac cagagtcaca gagtcacgat cgttagcaat tgcgtattcg
      841 atcgctgcac gaaccagacg tttggtgcct tcttccgaac acggcttaat accgatacca
      901 caatgttccg ggaagcgaat tttcttcacc cccatctctt cacgcaggaa tttaatcact
      961 ttctcggcgt cggcagagtc tgctttccat tcgatacccg cataaatgtc ttccgagttt
     1021 tcacggaaga taaccatatc ggtcagttca gggtgtttaa ccgggcttgg agtgccttga
     1081 tagtaacgta ccggacgcag gcagatgtag agatccagtt cctggcgcag ggcaacgttc
     1141 agagagcgaa taccgccacc aacaggagtg gtcagcggac ctttaatggc aacgcgatat
     1201 tcacgaatca gatcaagagt ttcagcaggc agccagacgt cctgaccata aacctgtgtg
     1261 gatttttcac cggtgtaaat ttccatccag gagattttac gctcgccttt ataggctttc
     1321 tcgactgcag cgtcgaccac tttcagcatg gctggggtta catctacacc gattccatca
     1381 ccttcaatgt aagggataat cggattttca ggaacgttga gtttgccgtt ttgcagggtg
     1441 atcttcttgc cttgtgccgg aacaactact ttactttcca ttcacctctc cttcgagcgc
     1501 tactggtttg ctcgtctgcc accacgttgc atatgcgttt gcgtcctgcg atacggatgc
     1561 tttagagcaa ttttttgtta atgatttgta attggcttgt caatactacc ctattgtttg
     1621 ccgtcatgaa agatgcgcgt tattaggcta taatgcggca attcataatc tctgaaaata
     1681 ccatgcaaaa aacttctttt agaaatcacc aagttaagcg attcagctcg caacgttcta
     1741 ccaggcgtaa acctgaaaac cagcccacgc gtgtgatcct gttcaataaa ccctacgatg
     1801 ttcttccgca gttcaccgat gaagccggac gcaaaacatt aaaagaattc atcccggttc
     1861 agggcgttta tgcagcaggt cgtcttgacc gcgatagcga agggttactg gtgctgacca
     1921 ataacggcgc gttgcaggcg cgtttaaccc agccgggcaa acgcaccggg aaaatctatt
     1981 atgtgcaggt ggaaggtatt cccacacaag acgcacttga agccttgcgc aatggcgtaa
     2041 ccttaaatga tggccctacc ctgcccgcag gcgcggagct ggttgacgaa cccgcgtggt
     2101 tatggccgcg gaatccacca attcgtgaac gcaaaagtat tcccaccagc tggctgaaga
     2161 tcaccttata tgaaggacgt aatcgccagg tgcgccgcat gaccgcccat gttggtttcc
     2221 ccacgctgcg actgattcgc tatgcgatgg gagattactc tttggataat cttgccaatg
     2281 gcgaatggcg agaagtgaca gattaaggat gcaaaatgtt taaaccgcac gttaccgttg
     2341 cttgcgtggt gcacgcagaa ggcaaatttt tagtcgttga agagacgatt aatggtaaag
     2401 cgttatggaa ccaacctgcc gggcatctgg aagccgatga aaccttagtg gaagccgccg
     2461 cccgtgaact gtgggaagaa accggcatca gcgcgcagcc gcaacacttt attcgtatgc
     2521 atcagtggat tgcgccagat aaaacgccgt ttttgcgttt cctctttgcc attgagcttg
     2581 agcaaatatg cccgacccag cctcatgaca gcgatatcga ctgctgtcgt tgggtcagcg
     2641 ccgaagaaat tttacaggcg tcaaatcttc gttcgccgct ggtggcggaa agtatccgtt
     2701 gttatcaaag cgggcaacgc tatccgctgg agatgattgg cgattttaac tggcctttta
     2761 caaagggtgt catctaaaag gctgcgtgat agaatacgcc gccttgaagt tcaatgtcgt
     2821 gagtgatcca atgtctgaaa ccgcaaaaaa agtaatcgtc ggcatgtccg gcggtgtcga
     2881 ttcctccgtt tctgcctggc tgttgcaaca acagggatat caggtcgaag gcctgtttat
     2941 gaagaactgg gaagaagacg acggtgagga atattgcaca gcggcagcgg atctggctga
     3001 tgcccaggct gtctgcgaca agctcggcat tgaactgcac accgttaact ttgctgccga
     3061 gtactgggac aacgtcttcg aactgttcct tgccgaatat aaagccggtc gcacgccgaa
     3121 tccggatatt ctgtgcaaca aagagatcaa atttaaagcc ttcctcgaat ttgccgccga
     3181 agatttaggt gccgattata tcgctaccgg tcattacgta cgtcgggccg atgtcgatgg
     3241 caagagccgc ctgctgcgtg gtctggacag caataaagac cagagctact tcctttatac
     3301 gctcagccat gagcagattg cgcaaagcct gttcccggtc ggcgaactgg aaaaaccgca
     3361 ggtgcgtaag attgctgaag atcttggtct ggtcaccgcg aagaaaaaag actctaccgg
     3421 catttgcttc attggcgaac gtaaattccg cgagttcctg ggccgttatc tcccggcgca
     3481 accgggcaaa atcattaccg tcgatggcga tgaaattggt gagcaccagg ggctgatgta
     3541 tcacactctc ggtcagcgta aaggtctggg tatcggtggc accaaagaag gtaccgaaga
     3601 accgtggtat gtggtggaca aagacgtcga aaacaacatt ctggttgtcg ctcagggcca
     3661 tgaacacccg cggctgatgt ctgtcgggtt gattgcccag cagttgcact gggtcgatcg
     3721 cgaaccattc accggcacta tgcgttgcac ggtaaaaacc cgctatcgcc agaccgatat
     3781 cccttgcacc gtcaaggcgc tggacgatga tcgcattgaa gtgattttcg atgaaccggt
     3841 tgccgccgtg acgccgggcc agtctgccgt cttctataac ggtgaagtgt gcctcggtgg
     3901 cggtattatt gagcagcgtc tgccgctgcc ggtctgatta ttatctttac ttaaaacaag
     3961 gaagcagtga acgtggcaaa gaattactat gacatcaccc tcgccctggc cggtatttgt
     4021 cagtcggcac gcctggtgca acaactcgct caccaggggc attgtgatgc cgatgcgcta
     4081 cacgtctcac tcaacagtat tattgatatg aaccccagct cgacgctggc ggtttttggc
     4141 ggtagcgaag ccaacctgcg cgtcgggctg gaaaccctgc tcggcgtgct caatgccagc
     4201 agtcgccagg gcttaaacgc cgaattaacc cgctacacac tcagcttgat ggtgcttgag
     4261 cgcaaactct cctcagcgaa aggcgcgctc gacactctgg gcaaccggat caacggcctg
     4321 caacgccagc tcgaacactt cgatttacag tccgaaacgc tgatgagcgc gatggctgct
     4381 atctatgttg atgtgatcag cccgcttggc ccgcgcattc aggtcaccgg ttcccctgct
     4441 gtactgcaaa gcccacaagt gcaggcgaaa gttcgcgcaa ccctgctggc aggcattcgc
     4501 gccgccgtgc tctggcacca ggtcggcggc ggacgtctgc aactgatgtt ttctcgtaat
     4561 cgcctgacca ctcaggcaaa acaaattctt gctcatttaa ccccggagtt gtgatctatg
     4621 gaattatcct cactgaccgc cgtttcccct gtcgatggac gctacggcga taaagtcagc
     4681 gcgctgcgcg ggattttcag cgaatatggt ttgctgaaat tccgtgtaca agttgaagta
     4741 cgttggctgc aaaaactggc cgcgcacgca gcgatcaagg aagttcctgc ttttgctgcc
     4801 gacgcaatcg gttaccttga tgcaattgtc gccaatttca gcgaagaaga tgccgcacgc
     4861 atcaaaacca tcgagcgtac cactaaccac gacgttaaag cggttgagta tttcctgaaa
     4921 gaaaaagtgg cggagatccc ggaactgcac gcggtttctg aattcatcca ctttgcctgt
     4981 acttcggaag atatcaataa cctctcccac gcattaatgc tgaaaaccgc gcgtgatgaa
     5041 gtgatcctgc cgtactggcg tcaactgatt gatggcatta aagatctcgc cgttcagtac
     5101 cgcgatatcc cgctgctgtc tcgtacccac ggtcagccag ccacgccgtc aaccatcggt
     5161 aaagagatgg caaacgtcgc ctaccgtatg gagcgccagt accgccagct taatcaggtg
     5221 gagatcctcg gcaaaatcaa cggcgcggtc ggtaactata acgcccatat cgccgcttac
     5281 ccggaagttg actggcatca gttcagcgaa gagttcgtca cctcgctggg tattcagtgg
     5341 aacccgtaca ccacccagat cgaaccgcac gactacattg ccgaattgtt tgattgcgtt
     5401 gcgcgcttca acaccattct gatcgacttt gaccgtgacg tctggggtta tatcgccctt
     5461 aaccacttca aacagaaaac cattgctggt gagattggtt cttccaccat gccgcataaa
     5521 gttaacccga ttgacttcga aaactccgaa gggaatctgg gcctttccaa cgcggtattg
     5581 cagcatctgg caagcaaact gccggtttcc cgctggcagc gtgacctgac cgactccacc
     5641 gtgctgcgta acctcggcgt gggtatcggt tatgcgctga ttgcgtatca atccaccctg
     5701 aaaggcgtga gcaaactgga agtaaaccgt gaccatctgc tggatgagct ggatcacaac
     5761 tgggaagtgc tggctgagcc aatccagaca gttatgcgtc gctatggcat cgaaaaaccg
     5821 tacgagaagc tgaaagagct gactcgcggt aagcgcgttg acgccgaagg catgaagcag
     5881 tttatcgatg gtctggcgtt gccagaagaa gagaaagccc gcctgaaagc gatgacgccg
     5941 gctaactata ttggtcgagc tatcaccatg gttgatgagc tgaaataaac tcgtatcagt
     6001 gccggatggc gatgctgtcc ggcctgttta ttaaaattat ccactttttc ttttttcact
     6061 ttacctcccc tccccgctgg tttatttaat gtttaccccc ataaacacat aatcgcgtta
     6121 cactatttta ataattaaga cagggagaaa taaaaatgcg cgtactggtt gttgaagaca
     6181 atgcgttgtt acgtcaccac cttaaagttc agattcagga tgctggtcat caggtcgatg
     6241 acgcagaaga tgccaaagaa gccgattatt atctcaatga acatttaccg gatattgcga
     6301 ttgtcgatct cggattgcct gacgaggacg gtctgtcact gattcgccgc tggcgtagca
     6361 atgatgtttc actgccgatt ctggtattaa ccgcccgtga aagctggcag gacaaagtcg
     6421 aagtattaag tgccggtgct gatgattacg tgactaaacc gtttcatatt gaagaggtga
     6481 tggcgcgaat gcaggcatta atgcggcgta atagcggcct ggcttcacag gtcatttcgc
     6541 tacccccgtt tcaggttgat ctctctcgcc gtgaattatc tattaatgac gaagtgatca
     6601 aactgaccgc gttcgaatac accattatgg aaacgttgat acgcaataat ggcaaagtgg
     6661 tgagcaaaga ctcgttaatg ctccaactct atccggatgc ggagctgcgg gaaagccata
     6721 ccattgatgt actgatggga cgtctgcgca aaaaaattca ggcacaatat ccgcaagaag
     6781 tgattactac cgttcgcggc cagggctatt tgttcgaatt gcgctgatga aaaaattact
     6841 gcgtcttttt ttcccgctct cactgcgggt acgttttctg ttggcaacgg ctgcggtagt
     6901 actggtgctt tcgcttgcct acggaatggt cgcgctgatc ggttatagcg tcagtttcga
     6961 taaaactacg tttcggctgt tacgtggcga gagcaatctg ttctataccc ttgcgaagtg
     7021 ggaaaacaat aagttgcatg tcgagttacc cgaaaatatc gacaagcaaa gccccaccat
     7081 gacgctaatt tatgatgaga acgggcagct tttatgggcg caacgtgacg tgccctggct
     7141 gatgaagatg atccagcctg actggctgaa atcgaatggt tttcatgaaa ttgaagcgga
     7201 tgttaacgat accagcctct tgctgagtgg agatcattcg atacagcaac agttgcagga
     7261 agtgcgggaa gatgatgacg acgcggagat gacccactcg gtggcagtaa acgtctaccc
     7321 ggcaacatcg cggatgccaa aattaaccat tgtggtggtg gataccattc cggtggagct
     7381 aaaaagttcc tatatggtct ggagctggtt tatctatgtg ctctcagcca atctgctgtt
     7441 agtgatcccg ctgctgtggg tcgccgcctg gtggagttta cgccccatcg aagccctggc
     7501 aaaagaagtc cgcgaactgg aagaacataa ccgcgaattg ctcaatccag ccacaacgcg
     7561 agaactgacc agtctggtac gaaacctgaa ccgattgtta aaaagtgaac gcgaacgtta
     7621 cgacaaatac cgtacgacgc tcaccgacct gacccatagt ctgaaaacgc cactggcggt
     7681 gctgcaaagt acgctgcgtt ctctgcgtag tgaaaagatg agcgtcagtg atgctgagcc
     7741 ggtaatgctg gagcaaatca gccgcatttc acagcaaatt ggctactacc tgcatcgtgc
     7801 cagtatgcgc ggcgggacat tgctcagccg cgagctgcat ccggtcgccc cactgctgga
     7861 caatctcacc tcagcgctga acaaagtgta tcaacgcaaa ggggtcaata tctctctcga
     7921 tatttcgcca gagatcagct ttgtcggtga gcagaacgat tttgtcgagg tgatgggcaa
     7981 cgtgctggat aatgcctgta aatattgcct cgagtttgtc gaaatttctg caaggcaaac
     8041 cgacgagcat ctctatattg tggtcgagga tgatggcccc ggtattccat taagcaagcg
     8101 agaggtcatt ttcgaccgtg gtcaacgggt tgatacttta cgccctgggc aaggtgtagg
     8161 gctggcggta gcccgcgaaa tcaccgagca atatgagggt aaaatcgtcg ccggagagag
     8221 catgctgggc ggtgcgcgga tggaggtgat ttttggtcgc cagcattctg cgccgaaaga
     8281 tgaataaata tgtccatact tcacgcatta cgttaagcat ccgttataat cggttgcaga
     8341 taccagcctg tggatgctta acatggaata ccaactcact cttaactggc ccgattttct
     8401 tgaacgtcac tggcagaaac gcccggtggt gttaaaacgc ggctttaata attttattga
     8461 cccgatctct ccagacgagt tggcgggtct ggcgatggaa agcgaagttg acagtcgact
     8521 ggtcagtcac caggatggca aatggcaggt cagccacggc ccgttcgaaa gctacgatca
     8581 tctcggtgaa accaactggt cattactggt acaggcagtg aaccactggc atgagccgac
     8641 cgccgcgctg atgcgaccgt tccgtgaact accggactgg cgtattgatg atctgatgat
     8701 ctctttttct gtaccgggcg gcggcgtcgg cccgcatctc gatcagtacg acgtgtttat
     8761 cattcagggt accggtcgtc gtcgctggcg agtgggcgaa aagctgcaaa tgaaacagca
     8821 ctgtccgcac ccggatctgt tacaggtcga tccgttcgaa gccatcatcg atgaagagct
     8881 ggagcctggc gatattcttt atattccgcc aggattcccg catgaaggct acgcgctgga
     8941 aaatgcgatg aactattccg tgggttttcg cgcgccaaat acgcgggaat taattagcgg
     9001 atttgccgat tatgtgctgc aacgtgaact gggcggcaac tactacagcg atccggatgt
     9061 tccacctcgc gctcatcctg cggacgttct gccgcaagag atggataaac tgcgtgagat
     9121 gatgctcgaa ttgatcaacc agccggaaca ctttaagcaa tggtttggcg agtttatatc
     9181 ccagtcacgt catgaactgg atatcgcgcc gccggaaccg ccttatcagc cagatgaaat
     9241 ctacgatgcg ctgaaacaag gtgaagtgct ggtgcgcctg ggtggtctgc gcgtattgcg
     9301 cattggcgac gacgtgtatg ccaatggtga gaagatcgat tccccgcacc gtccggcact
     9361 ggatgcactc gccagcaaca ttgcgctgac tgcggagaat tttggcgatg cgctggaaga
     9421 tccgtcattc ctcgcgatgc tcgcggcgct ggtcaatagc gggtattggt tcttcgaagg
     9481 gtaagtttgg atttaagccg gatgcggcgc tgattgcgcc ttatcgtgcc tacatgcact
     9541 gaacgcctgt aggcacgata agtatgtgga ccagtatcaa tataacggtt tcatcccggt
     9601 aattggcagt ccacatacgc atcgggcaat cacgacttcc gttgcgccgt taactcggca
     9661 atacggacaa tcacctgcac cgctttttcc ataccttcca gagtcacaaa ctcatgctta
     9721 ccatgatagt tgtaaccgcc agtgaacagg ttcgggcacg gtaatcccat aaacgacaac
     9781 tgcgcgccgt cggtaccgcc gcggatcggt ttcagttccg gttcaatatc gcagtcacgc
     9841 atcgcctgct gggcgatatc gataatatgc ggatgctcaa ccactttctc gcgcatattg
     9901 tagtaactgt cttcaatcac cagttcaatg tagcaatcag gatgtaaccc tttgcccact
     9961 tttttggcga tctccatcat tttacgttta cgcgcttcaa actgtttacg gtcgaaatca
    10021 cggatgatgt agtgcatatc ggctcggtca acggtgcctt tcatgctcgc caggtgatag
    10081 aaaccttcat agccttctgt catttccggg ctttcatccg ccggaacttc cgcatgaata
    10141 cgtgccgcca gcgacagcgc atttaccatc actcctttcg ccgtgcccgg atgaacattg
    10201 ttaccgacaa ttttgatatt gaccgatgcg gcgttgaagt tttcaaactc cagttcgcct
    10261 acgccaccac cgtcaacagt gtaagcccag cgggcatcga aggcatcaac atcaaaatgt
    10321 ttcgcccctt tgcccacttc ttcatccggg gtaaaggcga cgcgaatatc accatgcgga
    10381 atgtttttct gttgcaatac cgccagcgcg gtcatgattt ctgcaatacc tgctttgtca
    10441 tcggcaccta acaaggtttt accatcggtg gtaatcaacg tctgacccag tagctgatgc
    10501 agcaccggga acataaccgg tgataaaact tcatcgccga tacccagcgc aatatcgcca
    10561 ccgcgatagt tttcaacaat ttgcggattc acatttttgc cgctgcaatc cggtgaggta
    10621 tccacatgag aaataaagcc aatcgccggg atatcgccag ggacgttagc cggtaacgtc
    10681 gccatcaaag tgcccttctc acttaaggtc acattgataa gccccatctc ttcgagctgc
    10741 tctttcagca gatgcaataa cttccattgg ccttccgtgc tgggaacctg tctcacccct
    10801 gcttttgatt gggtatccag agacacgtag ttcaaaaatc gctcaagtag tttatccatg
    10861 tagtcaccct cactttttgt gacaacatta ttaagaagca agaaaagaca aatattgcgt
    10921 caggtcactt ttacccctgc aagcgggaat atttatcagc attaccttaa tgaatataaa
    10981 gctaagccag taattcacaa caaagattgg cgattcaagc ggtttgccgt agaattaccg
    11041 ccctcattaa gagtcaccaa ggtggttaac cacaaacccc gcatcggtaa gccatccgtt
    11101 gcgtttacat gggacagagt aaaaaattga ataaacaacc gagttcgctt tcaccgctgg
    11161 tgcaattggc gggaattcgc aaatgctttg atggtaaaga ggtcattccc cagctggatc
    11221 tgactatcaa caatggcgag ttcctcacgc tgcttggccc ttctggctgc ggtaaaacaa
    11281 ccgttcttcg cctgattgca ggtctggaaa ctgttgattc cggacgcatc atgctggata
    11341 acgaggacat cacccacgtt ccggcggaaa accgctatgt gaacactgtt ttccaaagct
    11401 acgcactttt cccccacatg accgtgttcg aaaatgtggc ctttgggttg cgcatgcaaa
    11461 aaacccccgc tgctgaaatt acgccccgcg tgatggaagc cctgcggatg gtgcaactgg
    11521 aaaccttcgc tcaacgtaaa ccgcatcagc tctctggtgg tcaacagcaa cgcgtcgcca
    11581 ttgctcgcgc ggtggttaac aagcctcgtc tgttgttgct ggatgagtcg ctctcagcgc
    11641 tggattacaa actgcgtaag caaatgcaga acgagctgaa agcgttacag cgtaagcttg
    11701 gtattacctt cgtctttgtc actcacgatc aggaagaagc gctcaccatg tcagacagga
    11761 ttgtggtgat gcgcgagggt cgtattgagc aagatggtac accgcgcgaa atttacgaag
    11821 agccgaaaaa cctgtttgtt gccggcttca ttggcgaaat caatatgttt aacgccactg
    11881 tcatcgaacg tctagacgag cagcgcgtac gcgccaacgt tgaaggccgc gaatgtaata
    11941 tctacgttaa cttcgccgtt gaaccggggc aaaaactgca tgttctgctg cgcccggaag
    12001 acttacgtgt tgaagagatt aacgacgaca accacgctga agggctgatt ggttacgttc
    12061 gtgagcgtaa ctacaaaggc atgacgctgg agtcggttgt tgaactggaa aatggcaaga
    12121 tggtgatggt cagcgaattc ttcaatgaag acgatcctga ctttgaccac tctctcgacc
    12181 aaaaaatggc cattaattgg gtagaaagct gggaggtcgt actggctgat gaagaacaca
    12241 agtaagttcc agaatgtagt gattgtcact attgtcggtt ggcttgtgtt gtttgtcttt
    12301 ctgcccaacc tgatgatcat tggcaccagc tttttgaccc gcgacgacgc cagtttcgta
    12361 aaaatggtct ttacgctgga taactacacg cgtctgctcg atccgctcta ttttgaagtg
    12421 ctattgcact cgctgaatat ggcgctgatc gccaccctcg cctgcctggt gctgggctac
    12481 ccgtttgcct ggtttctggc gaagctgcca cacaaggtgc gtccgctgct gctgtttctg
    12541 ctgattgttc ccttctggac caactcattg attcgtatct acgggctgaa aattttcctc
    12601 agcaccaaag gctatctcaa cgagtttttg ctctggctgg gcgttatcga cacaccaatc
    12661 cgtatcatgt tcacgcccag tgcggtgatt atcggtctgg tttacattct gctgccgttt
    12721 atggtgatgc cgctgtactc cagtatcgaa aaactggata agccgttgct tgaggcggcg
    12781 cgcgatctcg gtgccagcaa gttacagact tttatccgta tcattattcc actgacgatg
    12841 ccgggaatta ttgccggatg tctgctggtg atgctgccag cgatgggcct gttctatgta
    12901 tccgacctga tgggcggtgc gaaaaacctg ctgatcggta acgtcatcaa ggtacagttc
    12961 ctcaatattc gtgactggcc atttggtgca gccaccagca ttacgctgac tatcgtaatg
    13021 ggcctgatgt tgctggttta ctggcgcgct tctcgtctgc tgaataagaa ggtgaacgaa
    13081 ttagatgatt aatcctttat attcaattta ttaggtttgt ttttgagtga ttttgtcgtt
    13141 taaggtaacg gcgttataga tcctatcaaa atcaatcaat tgcattctgg ttttggagaa
    13201 agatttttcg agagcttcgg tctttaacta aaactgattg cgtataatcg caataccccc
    13261 tccccgacta tctgaatggt cggggaggca cttcaagcac aaaatatcat ccctgctccg
    13321 ttgattactc tacaggtgaa gtcggccact caatatcagg tgctgctgat gtatcaacac
    13381 gattcaacaa cacccgatac tttttccagg cttcccgcaa tgaagtctct tcctcaatta
    13441 cgatctccag atctgcagca tcctgaagtg gggcgcacca cgcaaacatc acaaaatatt
    13501 gtcgttgtga tggacctgtg caatagtgaa tcacgctacg tttctgtgta caacgtactg
    13561 tagttttctt tacgatttat agctgtactg gtgagccatg agtaatctga atccatgcat
    13621 ggcgtgtggt gcctgttgtg catttttctg cgtctctttt actggtccga agccgatgat
    13681 gctgaagcag tcctctcctc tagcctcatc cctgtatagc acgcgtcaag taagctggga
    13741 tatcatttcg tagttttaca atatcaatat ccgaatcgat atgtttctgg taaaacttgt
    13801 ccgcaagcaa gtttgctatg gtatctgatg tacgattttt tacatcccgg ctcataccat
    13861 cacaaagagc atccattttc ttgccaaaag aagcactccc cattattaca tgattaatag
    13921 aactttgtgc tgccttaaca tcaagggtta gttgattctc ctggctcaca agagaaaaga
    13981 ctctttgcct ctcttcagtg gaaatttttg catttttttg aaaacagtaa ttatcaatct
    14041 cctggttgat cacccttgag ggatatacta tgcgttcatc ttttatccac ccatcaatat
    14101 ctttagcact ctgcctgttc actgtatcca gtatatttct gctaaatttg cgaacagaaa
    14161 agctaatagt ccgttctcca attgaacatt ttacgacctc atcattcttc cctcggaaag
    14221 agttcctaaa ggagccttct ctatggcaac ttataccaaa agagctgaaa gacgtacctg
    14281 aagtattcat cggcattata attccttttt ataaaaaagc gtttacaaga ttgcatgctt
    14341 gtgtttattg tagccagaaa aaacatgcat gcaaataata ttctattaaa agttgtattg
    14401 tttgagtccc atctcatgat cggtatgttg actcttgtta ccaatatagt tcacaaaatg
    14461 ggttgaaaag gataataata tgccagtcat attaaatttt tctaatggaa gtgtattgcc
    14521 ggaaaatgag ctggaggctt tacgacatat tgcaagaagc aatcagaatg acactattac
    14581 tataggaggc cgtaatatga ggcttcatta tatccagttt atggatggtt ttagtgtaga
    14641 gcctattcct ggtggacttt gggaccatct tggggcaaga gagtctcatc atctcgcaga
    14701 cagcctggca agacaactta acggaggtaa cacttttctg caggcataca gcttatatct
    14761 ggagcagagg caagctgccc cgcttgtgca ggaaagcgtc ataaaaacac tactagatcg
    14821 aataaattcg aatgcatttc ccgttagttt acaagacttt tcctgtactg aggagcatct
    14881 taattgtccg ataacgctac atattcctga gacaggtgtt tttgtcagaa atgctcgaaa
    14941 ttcagaaata tgtgcattat atgatcagga agcattgact gaacttatcc tgcgtaacgc
    15001 tctccacccc ctcagccgtg acccctttgc tccagaaatg attataagca aagacaagtg
    15061 tcattttaat ataacaaaac aatgttttta cgcattaccc atataccccc ttcaacaaaa
    15121 cagtatttaa aaataaaaac acaatataag aagcatacaa taagttaatc accatataac
    15181 aaacatatag cgaggagatt atttatgccc aaaatatcat cagttgtatc atcatgttac
    15241 catctgttca gtgaacatca acaactttca aatgaaacaa caatgacgaa ctccgtctcc
    15301 agaagaattg ttcataaaga atatggtata tctttaaaat ccgttcctgt atggttggct
    15361 acagctaaaa ctccccttgc tctactcaat ggcagacata caagaagtca ctcatttatt
    15421 attgcaggga ctccaggaat gggaagcagg agcggagccc aatactatgc cataaacagt
    15481 g
//
LOCUS       DAZFWL020000110        15401 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFWL020000110 DAZFWL020000000
VERSION     DAZFWL020000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.110"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(349..1173)
                     /gene="iclR"
                     /locus_tag="ACHKNP_RS18185"
                     /old_locus_tag="pgaptmp_003637"
     CDS             complement(349..1173)
                     /gene="iclR"
                     /locus_tag="ACHKNP_RS18185"
                     /old_locus_tag="pgaptmp_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HGU4705731.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1373..5056
                     /gene="metH"
                     /locus_tag="ACHKNP_RS18190"
                     /old_locus_tag="pgaptmp_003638"
     CDS             1373..5056
                     /gene="metH"
                     /locus_tag="ACHKNP_RS18190"
                     /old_locus_tag="pgaptmp_003638"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HGU4705732.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYCESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5276..6907
                     /locus_tag="ACHKNP_RS18195"
                     /old_locus_tag="pgaptmp_003639"
     CDS             5276..6907
                     /locus_tag="ACHKNP_RS18195"
                     /old_locus_tag="pgaptmp_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGU4705733.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(6998..7687)
                     /gene="pepE"
                     /locus_tag="ACHKNP_RS18200"
                     /old_locus_tag="pgaptmp_003640"
     CDS             complement(6998..7687)
                     /gene="pepE"
                     /locus_tag="ACHKNP_RS18200"
                     /old_locus_tag="pgaptmp_003640"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HGU4705734.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8070..9299)
                     /gene="sorE"
                     /locus_tag="ACHKNP_RS18205"
                     /old_locus_tag="pgaptmp_003641"
     CDS             complement(8070..9299)
                     /gene="sorE"
                     /locus_tag="ACHKNP_RS18205"
                     /old_locus_tag="pgaptmp_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HGU4705735.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            complement(9351..10174)
                     /locus_tag="ACHKNP_RS18210"
                     /old_locus_tag="pgaptmp_003642"
                     /pseudo
     CDS             complement(9351..10174)
                     /locus_tag="ACHKNP_RS18210"
                     /old_locus_tag="pgaptmp_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(10185..10982)
                     /locus_tag="ACHKNP_RS18215"
                     /old_locus_tag="pgaptmp_003643"
     CDS             complement(10185..10982)
                     /locus_tag="ACHKNP_RS18215"
                     /old_locus_tag="pgaptmp_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HGU4705736.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            complement(11048..11542)
                     /locus_tag="ACHKNP_RS18220"
                     /old_locus_tag="pgaptmp_003644"
     CDS             complement(11048..11542)
                     /locus_tag="ACHKNP_RS18220"
                     /old_locus_tag="pgaptmp_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGU4705737.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            complement(11542..11949)
                     /locus_tag="ACHKNP_RS18225"
                     /old_locus_tag="pgaptmp_003645"
     CDS             complement(11542..11949)
                     /locus_tag="ACHKNP_RS18225"
                     /old_locus_tag="pgaptmp_003645"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HGU4705738.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            complement(11959..12765)
                     /locus_tag="ACHKNP_RS18230"
                     /old_locus_tag="pgaptmp_003646"
     CDS             complement(11959..12765)
                     /locus_tag="ACHKNP_RS18230"
                     /old_locus_tag="pgaptmp_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4705739.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(12835..13782)
                     /locus_tag="ACHKNP_RS18235"
                     /old_locus_tag="pgaptmp_003647"
     CDS             complement(12835..13782)
                     /locus_tag="ACHKNP_RS18235"
                     /old_locus_tag="pgaptmp_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGU4705740.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELRIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            14130..15002
                     /gene="rluF"
                     /locus_tag="ACHKNP_RS18240"
                     /old_locus_tag="pgaptmp_003648"
     CDS             14130..15002
                     /gene="rluF"
                     /locus_tag="ACHKNP_RS18240"
                     /old_locus_tag="pgaptmp_003648"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HGU4705741.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(15003..15275)
                     /locus_tag="ACHKNP_RS18245"
                     /old_locus_tag="pgaptmp_003649"
     CDS             complement(15003..15275)
                     /locus_tag="ACHKNP_RS18245"
                     /old_locus_tag="pgaptmp_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HGU4705742.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
BASE COUNT         3679 a         4011 c         4033 g         3678 t
ORIGIN      
        1 gagaaagaac tacgagggat acgagtaatc atttaaatat ctcattgttt attgatgtct
       61 tgaattttaa ctatcagaac agtgaaaaaa tttaatatga ttacaactaa agaaatatca
      121 taaatcgctc aatctcataa tgcagccgta aaagtttcgg tggaatgaga tcttgcgatt
      181 ttgttaatga ttaagtagtt tgatgtttaa aaataatgct gagttaataa aagtgtgtga
      241 agtgtatgac attaagttat tttggcgtca atgcgattaa cagacaccct tattctattg
      301 ccactcaggt atgatgggca gaatattgcc tctgcccgcc agaaaaagtc agcgcattcc
      361 accgtacgcc agcgtcactt ccttcgccgc tttaatcacc atcgcgccaa actcggtcac
      421 gcggtcatcg gtaatacgtg aaatcggtcc ggaaattgaa attgcggcaa acggttcgcg
      481 gtgctcatcg aaaatacacg ctgcaaggca acgtagcccc agtgcatgtt cctcatcgtc
      541 aaatgaataa ccccgtttgc gcgtttgggc gagatcttct tttaaatgca caggagacac
      601 cagcgttgcg tgggtatagg catgtaaccc tttgcggtgc agcagcttcg tcacctgttc
      661 ttcgctcagt tgggctaaaa aggctttacc cgcaccggaa gcgtgcatcg gcaatttacc
      721 gccgataggc gcggacattc gcatcagatg cgtacactgt acctggtcga taataatcgc
      781 ttcgtgatcg ctttgatcaa gcaccgccat attgaccgtt tcgccagact cttccattaa
      841 attgcgcagg atagggtgaa caatcgctaa caaattacgg ctctggagaa agctgctgcc
      901 gaccataaag gcatgtgcgc cgattgccca atgtcccagt tcgccgacct gacgcacgaa
      961 accctgctgt tgcatcgtgg ttagcaggcg gtgggtcgtg gaattgggta acccggcttg
     1021 ttgcgccagc tctgtgagtg ccacactgcc attggattcg gcaatccact ccagtaattt
     1081 caggccacgc gttaaagact gaacctgtcc agtcgctggt gcggtggcaa cggcgggttt
     1141 tctgccgcgt ttcgcgggaa tgggtgcgac catgacagcc tcctttttct gtatcgtgga
     1201 aatcattttc atttttattg ttagctaatg caatagttgc tgaactgatc cgatgagtta
     1261 atgttgaaca aatctcatgt tgcgtggtgg tcgcttttac cacagatgcg tttatgccag
     1321 tatggtttgt tgaattttta ttaaatctgg gttgagcgtg tcgggagcaa gtgtgagcag
     1381 caaagtggaa caactgcgtg cgcagttaaa tgaacgtatt ctggtgctgg acggcggtat
     1441 gggcaccatg atccagagct atcgactgaa cgaagccgat tttcgtggtg aacgctttgc
     1501 cgactggcca tgcgatctca aaggcaacaa cgacctgctg gtgctcagca aaccggaagt
     1561 gatcgccgct atccacaacg cctactttga agcgggcgcg gatatcatcg aaaccaacac
     1621 cttcaactcc acgaccattg cgatggcgga ttaccagatg gaatccctgt cggcggaaat
     1681 caactttgcg gcggcgaaac tggcgcgcgc ctgtgctgac gagtggaccg cgcgcacgcc
     1741 ggagaaaccg cgctacgttg ccggtgttct cggcccaacc aaccgcacgg cgtctatttc
     1801 tccggacgtc aacgatccgg cattccgcaa catcactttt gaccagctgg tggcggccta
     1861 ttgcgaatcc accaaagcgc tggtggaagg tggtgcggat ctgatcctga ttgaaaccgt
     1921 tttcgacacc cttaacgcca aagcggcggt ttttgcggtg aaaacggagt ttgaagcgct
     1981 gggcgttgag ctgccgatta tgatctccgg caccatcacc gacgcctccg ggcgcacgct
     2041 ctccgggcag accaccgaag cattttacaa ctcattgcgc cacgccgaag ctctgacctt
     2101 tggcctgaac tgtgcgctgg ggcccgatga actgcgccag tacgtgcagg agctgtcacg
     2161 gattgcggaa tgctacgtca ccgcgcaccc gaacgccggg ctacccaacg cctttggtga
     2221 gtacgatctc gacgccgaca cgatggcaaa acagatacgt gaatgggcgc aagcgggttt
     2281 tctcaatatc gtcggcggct gctgtggcac cacgccacaa catattgcag cgatgagtcg
     2341 tgcagtagaa ggattagcgc cgcgcaaact gccggaaatc cccgtagcct gccgtttgtc
     2401 cggcctggag ccgctgaaca ttggcgaaga tagcctgttt gtgaacgtgg gtgaacgcac
     2461 caacgtcacc ggttccgcta agttcaagcg cctgatcaaa gaagagaaat acagcgaggc
     2521 actggatgtg gcccgccagc aggtggaaaa cggtgcgcag attatcgata tcaacatgga
     2581 tgaagggatg ctcgacgccg aagcggcgat ggtgcgtttt ctcaatctga ttgccggtga
     2641 accggatatc gctcgcgtgc cgattatgat cgactcctca aaatgggatg tcattgaaaa
     2701 aggtctgaag tgtatccagg gcaaaggcat tgttaactct atctcgatga aagagggcgt
     2761 cgacgccttt atccatcacg cgaagctctt gcgtcgttac ggtgcggcag tggtggtaat
     2821 ggcctttgac gaacagggac aggccgatac ccgcgcacgg aaaatcgaga tttgccgtcg
     2881 ggcgtacaaa atcctcaccg aagaggttgg cttcccgcca gaagatatca tcttcgaccc
     2941 gaacatcttc gcggttgcca ccggcattga agagcacaac aactacgcgc aggactttat
     3001 cggcgcgtgt gaagacatca aacgcgaact gccgcacgcg ctaatttccg gcggcgtatc
     3061 taacgtttct ttctcgttcc gtggcaacga tccggtgcgc gaagccattc acgcagtgtt
     3121 cctctactac gctattcgca atggcatgga tatggggatc gtcaatgccg ggcagctggc
     3181 gatttacgac gacctgcccg ctgaactgcg cgacgcggtg gaagatgtga ttcttaatcg
     3241 tcgcgatgat ggcactgagc gtttactgga gcttgccgag aaatatcgcg gcagcaaaac
     3301 cgacgacacc gctaacgccc agcaggcgga gtggcgctcg tgggaagtga ataaacgtct
     3361 ggaatactcg ctggtcaaag gcattaccga gtttatcgag caggataccg aagaagcccg
     3421 ccagcaggct acgcgcccga ttgaagtgat tgaaggcccg ttgatggacg gcatgaacgt
     3481 ggtcggcgat ctgtttggcg aagggaaaat gttcctgcca caggtggtca aatcggcgcg
     3541 cgtcatgaaa caggcggtgg cgtatctcga accgtttatt gaagccagca aagagcaggg
     3601 caaaaccaac ggcaagatgg tgatcgccac cgtgaagggc gacgtccacg acatcggtaa
     3661 aaatatcgtt ggtgtggtgc tgcaatgtaa caactacgaa attgtcgatc tcggcgttat
     3721 ggtgcctgcg gaaaaaattc tccgtaccgc taaagaagtg aatgctgatc tgattggact
     3781 ttcggggctt atcacgccgt cgctggacga gatggttaac gtggcgaaag agatggagcg
     3841 tcagggcttc accatcccgc tattgattgg aggcgcgacg acctcaaaag cgcacacggc
     3901 ggtgaaaatt gagcagaact acagcggccc gacggtgtat gtacagaacg catcgcgcac
     3961 cgttggtgtg gtggcggcgc tgctttccga tactcagcgt gatgattttg tcgctcgtac
     4021 ccgcaaggag tacgaaaccg tacgtattca gcacgggcgt aagaaaccgc gcacaccacc
     4081 ggtcacgctg gaagcggcgc gcgataacga tttcgctttt gactggcagg cttacacgcc
     4141 gccggtggcg caccgtctcg gcgtgcagga agttgaagcc agcatcgaaa ctctgcgtaa
     4201 ttacatcgac tggacaccgt tctttatgac ctggtcgctg gccgggaagt atccgcgcat
     4261 tctggaagat gaagtggtgg gcgttgaggc gcagcggctg tttaaagacg ccaacgacat
     4321 gctggataaa ttaagcgccg agaaaacgct gaatccgcgt ggcgtggtgg gcctgttccc
     4381 ggcaaaccgt gtgggcgatg acattgaaat ctaccgtgac gaaacgcgca cccatgtgat
     4441 caacgtcagc caccatctgc gtcaacagac cgaaaaaacg ggattcgcta actactgtct
     4501 cgctgacttc gttgcgccga agctttctgg taaagcagat tacatcggcg catttgccgt
     4561 gactggcggg ctggaagagg acgcactggc tgatgccttt gaagcgcagc acgatgatta
     4621 caacaaaatc atggtgaaag cgcttgccga ccgtttagcc gaagcctttg cggagtatct
     4681 ccatgagcgt gtgcgtaaag tctactgggg ctatgcgccg aacgagaacc tcagcaacga
     4741 agaactgatc cgcgaaaact accagggcat ccgtccggca ccgggctacc cggcctgccc
     4801 ggaacatacg gaaaaagcca ccatctggga gctgctggaa gtggaaaaac ataccggcat
     4861 gaaactcaca gaatctttcg ccatgtggcc cggtgcatcg gtttcgggtt ggtatttcag
     4921 ccacccggac agcaagtact acgcagtggc gcaaattcag cgcgatcagg ttgaagacta
     4981 tgcccgccgt aaaggtatga gcgtctccga tgttgagcgc tggctggcac cgaatctggg
     5041 gtatgacgcg gactgattca caaatctgtc acttttcctt acaacaaaca gggcgctcaa
     5101 tgagtgccct gtctctttat taatatgaaa cacttatact ggaaacaggc tggaataatc
     5161 ttagccggaa aaacgaatgt caaaaggcac agtaaagatg ccagtcagcg tgtttccggt
     5221 cctttggtca ttcaattgct tcaggaacat ataacgataa ggagaacctg aactcgtgtt
     5281 aacgctgctt cacctgcttt ctgccgtcgc cctgctggtc tgggggactc atattgttcg
     5341 aaccggcgta atgcgcgtct tcggcgcgcg tttgcgtact gtccttagcc ggagcgtcga
     5401 aaaaaagccg ctcgcctttt gcgcggggat cggcgttacc gcactggtgc agagcagtaa
     5461 tgccaccacc atgctggtga cctcgtttgt cgctcaggat ctggtagccc tcgcaccggc
     5521 tctggtcatt gtgctggggg cagatgtcgg gacggcgcta atggcgcgta ttctcacctt
     5581 cgacttatcc tggctgtcac cgttacttat ttttatcggc gtgatttttt tcctcggacg
     5641 caaacagtca cgcgccgggc aactgggccg cgtcggtatt ggtcttgggc tgattttact
     5701 ggcgctggag ttgattgtgc aggccgtaac gccgatcacc caggcaaacg gcgttcaggt
     5761 gatctttgcc tcgctgaccg gcgatattct gctggatgcg ctgattggcg cgatgttcgc
     5821 cattatcagc tactccagcc ttgctgctgt actgctgact gcgactctga ccgccgcagg
     5881 cattatctcc ttccccgtgg cgctctgtct ggtgattggt gctaacctcg gttccggcct
     5941 gctggcgatg ctcaacaaca gtgccgccaa tgccgcagcc cgccgtgtcg cgctgggtag
     6001 tctgctgttt aagctggtgg gtagcctgat tatcctgccg tttgtccatt tgctggcaga
     6061 gacaatgggg aagctgtcat tgccaaaagc ggaactggtg atctatttcc acgtcttcta
     6121 caaccttgta cgttgcctgg tcatgctgcc atttgttgac ccgatggcac ggttttgcaa
     6181 aacgattatt cgcgatgaac cggaactgga tacccagcta cggcctaaac atctggatgt
     6241 cagcgcgctg gatacgccca cgcttgctct ggcgaacgcc gcgcgcgaaa ccctgcgcat
     6301 tggcgacgcc atggaacaga tgatggaagg gctgaataaa gtgatgcacg gcgagccacg
     6361 gcaggagaaa gagctgcgta agctggcaga tgatatcaac gttctctata ccgccattaa
     6421 gctgtatctg gcgcggatgc caaaagagga actggcagaa gaagagtcgc gtcgctgggc
     6481 ggagattatt gaaatgtcgc tcaaccttga acaggcttcc gatatcgtcg agcgcatggg
     6541 cagcgaaatt gccgataaat cgctggcagc acggcgggca ttttcgcttg atgggttgaa
     6601 ggaactggat gcgctctatg agcaattgct cagtaattta aagctggcaa tgtcggtgtt
     6661 cttctctggc gatgtcacca gcgctcgtcg tttgcgtcgc agcaaacatc gttttcgcat
     6721 tcttaatcgc cgctattccc acgcccacgt cgatcgcctg catcagcaaa acgtgcagag
     6781 cattgaaacc agttcgctac atttaggctt actgggagat atgcagcgcc tgaactcgct
     6841 gttttgttcg gtggcttaca gtgtgctgga acagccggat gaagatgaag gacgggacga
     6901 gtattaatat tgtgaatcct gtaggcctga taagacgcgt caagcgtcgc atcaggcact
     6961 atgaacaaga tgccggatca tgcatccggc aatttactta aaaacggtga ccagcttcta
     7021 gcggcaccgc ttcttcacca gccttaaaca cataagtggt gttcgggcca cccagcgtag
     7081 cgtgaccttt actcactgtg atccagttac cttccggtag accaataatc gtcagttctg
     7141 gcgcgacgac cagcagttcg cgaatacgct gctcacgggt ttcaccttta tggccttccg
     7201 gcagcgcgtt ggtgaagtgc gggttgattt gcagcgggaa cagatttagc gcatcgaaac
     7261 cttgcggatc gacaatcggc atatcgttgg tggtacgaat ggttgggcaa gcaaggttag
     7321 cgcctgcgct ccagccaata tacagcgcac cacgtttaac cacgtcagta attggtgcca
     7381 gcagcccgcg ctcgcggcac tgtttcagca actggaaagt attcccgccc ccgacaatca
     7441 cgatctcagc attttcaatc gcggcaacgg gatcgacaac gctatgaata ccggtgacag
     7501 atacacccag cggagcgaga accgcagccg ttttcgctgt gtaatcatcc caggtctgcg
     7561 ttacgccagc gaaaggaata aataccgctg aacggcgacc ctgcaactgt tcagcaatca
     7621 gcggcagcgc atgttccagc caggctttac ccggcagcgt cgagttactc aataaaagca
     7681 gttccattac ttctccagtt agtaagaaat cacgggaggc atgctaccac tgcgaattta
     7741 accacttact gacatggctc acgatgtcac gtattgcacc gggcgctgag actgatgaca
     7801 aacacagcat tgcctgatgc gctacgctta ccaggcctac cagataactg caatatattg
     7861 aattttcgag attatgtagg ccgaataagg ctttattccg catccggcat aaacaatgtg
     7921 tactttgcca ccaatttgag cgccccttaa ggcgctgatg tttttttatc cttctcgtgc
     7981 acattgggcc gcttcagcaa tcagccacac atcaggtcgc gaagttgccg cggcgaccag
     8041 ccagcgacat gcagagttaa gtagcgcgat taatcatggg aaattgcctc tgcatgagtg
     8101 agcagatatt gctccgcctc accggaccag atcccctgat gacgcgccag aatcgccgcc
     8161 agtgcttgat cctgaatctg cgtgagtgac gtcagcggca ggtatttccc ggtatacacc
     8221 agctttttgc cgccgccgac ggcaggcaat tcaagcgtgg tttcgcccgc ggcattcagc
     8281 ccaagaatat gtgttaccac ttttgcggcc tgcacttttt tctcttcaat cagtttgacc
     8341 gccgcgcgca tgtcgtcggt attgccgcct gacgtgccca cgtagtgggt aaatgcataa
     8401 tgcacatcgt agaaattaat tggcgcgctg aaatgtttat cctgcggtcc ggcgaagaaa
     8461 ttcaggcaac catctgtcgc cagcaaggaa gaggcgagag tcaccagtcc ttcattaggc
     8521 acaaagacga aaatatcatc gaagccgtga ccgccactca gcgccattag cgtatcaaat
     8581 gctgcatcgg cggcattgag ataatgaatc agtgtttgcg gttctgatgg atagtgcttg
     8641 cgcgcataac tcaatttatc gttgtcggta tcggtaatga cgagcagcga cgggttaacg
     8701 ggtccatgta gcgcataatc aatcgccaac agtcccattg gtccggtgcc gccgaggatc
     8761 agcatgcgcc cttgcgggcg aatccccatc gtgtggttat aactaccttc ctgaagatga
     8821 tagttggcgt tgaacgcgcc aatcacgcag gaaagcggtt caaccagtga gccttcaaaa
     8881 taggtttcgc cgtcgtatgc cagcaggcaa tcttgttcca tgacctcgtt gggaataacc
     8941 acatgcgtgg cttcgccgcc cacccacggg aaggagtagc cggggcagtc cgggcggtcg
     9001 gggagttgca ggttggcctg aatcacataa cgctgacccg gctggaattt gtgctgccac
     9061 tttttaccca cggccagaat atcgccgcaa aactcgtggc cgatgatgat ggggttggtc
     9121 gccacatcgt cgggtacttt tttatggttt tcacccagat tggcctcttt ccaggaagag
     9181 aggcacaggc tgtcagtgac caccgtcgcg aggatttcat cctcctgcat ttcaggaagg
     9241 tcaaaggttt ccaggcgtaa atcacgttta ccataaagac gcagagctgt tgttttcata
     9301 aaccacctca tgttgagcga tggccccggc agtcgtgccg gggcgaagaa tcaggacagg
     9361 aagcccagcg cattaccgat aatccctaag ccaaacaggg cgaaaatcag ccataccggg
     9421 ttaatttttt tgttgagcag acgaaccata agcagcgtca gaccgagcgc cagcaaacca
     9481 gggcaaagtt ggtcgagaat gttctgcacg gtcatggtga cggtggagcc atcggcggca
     9541 tgcgtttgtg aaaccaccaa cggtacgttg attgacgtcc atttggtgac cagcacgccc
     9601 atcacaaaca gtccgagaat cgacgcgcct tcggtgagtt tttgcagcac attcccgccc
     9661 atatcgctga cgatattcac ccctttgcga aagccgagct gtaggccata ccacttcatc
     9721 gccagacgca ccgcattgaa aataaagaaa aaaaaagcgg gccgaggatg ttgccagaaa
     9781 gtgccagaga tgcgccgagc gcagcggtaa tcgggcgcag cgttccccaa accagtggat
     9841 cgccaacccc tgccaatggt cccatcagac cgactttgat gccgttaatg gtaccgtcat
     9901 caatttccgc gccgttggcc cgcgcctctt ccatggcggc agtgacgccg atgaccgggc
     9961 cacatacggc tggcgtggta ttgaagaaca ccaggtgtcg cctgagcgcc gcaacctgat
    10021 cctcttttaa tgggtaaagt cgcttgatgg cggggatcat gtcgtagcaa aagcccagcc
    10081 cgtgaatacg ttcaaagtta aaggacgcct gttgcaggtt ggagcgcaga aacatgctca
    10141 ccagatcgct gcgtgtaatt tttctctgtt ccatgatggc tccgttaatc gtcaagttgg
    10201 tcaagggcgg tggaggtggt ggcctggggt tgaggttcag ctttacgcca ctgtggattt
    10261 agctggatgt agaccagggc catgatcacg ccgacgccac cgaacgccag cagactgaga
    10321 tcgaggtagc cacctgcgag gaaaccgagg aaaaagaaag gcatcaaata tttcacgccc
    10381 atcatacgta gcaccatggc gtaaccgacc accacgataa aaccgccagc aatttgcaga
    10441 ccgcgcgtca cgaattccgg tatcgcgctc agcatattgc tcaccatatc ggcgctgacg
    10501 aacagtgaga caatcagtgc cggaatagca acgcgcagcg cctgcacgcc aagcgcggag
    10561 acatgcagaa tatcgagagt gcgaaaccgc gcttcttccg ctgctttatc cgccgcgtgc
    10621 tggaacacta cggtgatggt acgggcaaac acagtcagca cctggcctgc cgcagccaca
    10681 ggcaacgcga tggcgattcc ggtggcgatg ctttgctgac caacgataac cagaatggcg
    10741 gagattatgc tggcgagtgc agaatccgga gattgcgccg cgccgacgtt catccagccg
    10801 agagctatca attccagcgt accaccgagt attattccgg tttttaaatc accgagaatt
    10861 aaaccaatca ccgtacaggc aattaacggg cgatgagtct gaaattcatc cagcacgctg
    10921 cccattccgg caatacagga aaaaagaaat atagcaatga tttgtagggt acttatttcc
    10981 atgatttttc accttttaag tgtcgtcatc cctgcctgtt aaagcatatg tacacaggcg
    11041 ctattattta atttgcgatt aactgttcgt ttattttgtc gataatattg attgagggat
    11101 ctgacgctac gacgcgtaaa tccagtatga cgccaagatt atttaactca tgaaatgcgt
    11161 taatatcatc atcatctaat gaaaccgctt tggttaactg ttttttaccg ggccgccagg
    11221 ccatgccgcc aatattcagg gtgtctattt ttaccccctg gcgaaccatc gctaaggcat
    11281 cctgtgggcg agtgaaaaga taaaaaacgg tttcgtcctg atattgcggg ttgtgataaa
    11341 cagcgacggc tttttcaata ttgaccacgt taaccttcat tcccggtggc gcagcctgac
    11401 gcaataatgt ccggcgaact tcatcgttat aaacttcgtc attgcagata ataatgcgct
    11461 gggcgttagc cactttcgac cagacggtag tgacctgacc gtggatcagg cggtcatcaa
    11521 tgcgggcgag cgtaatgttc atcagaaatc ctcctctgcc gttgccagtt gtttccagac
    11581 aacgcaggtt tgctttgcga tttgcgtcag gtgttcacac agttcatcaa cattcatgct
    11641 gtcctggtta tcaaccagtt cgagcgccag cggcagagaa aggccgctga ttacccgcag
    11701 acgaggattg cgcatcgcaa gcatcgctgc cgcattccac gggctgccgc actgtaaatc
    11761 cacggcaatc agccactcat cttgattgtg aatgcttact aacttttcca gctttgctac
    11821 gatgtctccg gcgttctcgc cgggcacaaa ttcaacggct tcaacattgg cgttgccgta
    11881 aaccatctga accgattcca gcatggcgca agctaatttg ccgtgggcac aaaaaatggc
    11941 gttgaccata aagcctcctt agccgcgcgt tttgccgccc gcaatgttag tggttactcc
    12001 ggtgatgtag ctggcgcgtt cagacaacag ataacaaaca aaatcagcga cttctgctaa
    12061 tcttccggcg cgcccaatag gaatggcgtt tttggtatag ccttcacgca gctgctcgac
    12121 ggtgatattg cgcgtccacg ccagcgcctc ttcatattcc ggcgtgcgta gcccggtttt
    12181 ttccagaatc ccgggcgcga taccgaccac acggataccg tgtttaccca gctctttcga
    12241 ccaggagcgc gtgaagctat taagcgcggc tttggtagcg gcgtaacagc tttggccttc
    12301 tgagccttcc agtccacttt ctgaggaaac attcacaatc acgccatcat gttgtttgac
    12361 catctgtcgc gccaccgcct gcgacatcag aaaaacgcct ttctggttga tattgaccat
    12421 tttttcgaat gcagcttcgt tgagttcata ctgcccggca ggcgctttct catcgaccag
    12481 cagacgcggg aaattgaccc cggcgttatt gaccagaccg tcgatgcgac caaaacgctg
    12541 gataatttcc gctaccgtat gatttacctc tttggcgctg gaaatatcgg tcggccaaaa
    12601 ctgatagcct ttatgacctt catattgacc atcgccaccg tgaatatcta ccatctgtac
    12661 gtttgcgcct tgtgctaata attcctctac aatcgccaga ccaataccgg atgcgccgcc
    12721 ggtgacgata atgattttat cctgcaaatt taaccacgtt tgcataacag actcctgatt
    12781 ttagataata gggacccgcc gtaattttta ttacggtaaa catcgggaag atttttattt
    12841 cagtaatagt tcagctgtgc tgctattcgt taccagacaa ttaatatatt ttccacgcag
    12901 tgcaccaata attccgctgt atttttcttc actcatggca atgccaatgg aatatcgcgc
    12961 ctgctttaat ttattcattt cgatagagag cgttttttcg ctcatattcg tttcaaccat
    13021 tgcgccgtga atatcaaaaa agcgcgagca aatatcgcca gcaacctggc gggcattcag
    13081 gtcgtcactc tcttcaccac cataaaacgc atgccagtta gcgccgtcgc gaatggccgg
    13141 tgagccaatt cccaccaggg cgacatccag attatcccag taggcagaga tggttttaaa
    13201 gtgctgagac tgcatgatcc cattacgaat taatgggtta tccagcagag ccggaaaatc
    13261 cgcgagatgc gattcccctt tcagcttcgc tgccgcgctg taggttaatg tgtttacatg
    13321 atagcggctt tcgagtttac cggacggtcc gccaataatt ggtacgcaga ttaactgccg
    13381 cgattgcccc gcctgcggca agttttcaac cagtgcgctc accgcgcgac cccaggaaaa
    13441 accgacaata tcgccaggct ccagcaagcg atccagcagt tgcgcgccgt gtaaccccat
    13501 catcgccagt tgagtctctt catcctcatc atttcccgac accaccacaa cgtctttcag
    13561 gccaaacttt tgcttcagtt gctgctccag ccagagattt tcgttgtagt catagttgat
    13621 ggcgatggtg acaattccct gatcgcggcc tcgtttaagc aagcggctga tggtggtgcg
    13681 gtaaatacgc agttcgcgcg cgatttgcgc ctgcgtcata tcctgttcgt aatagagttg
    13741 ggcaatcttc acaatcaaac ggatatcgtc actgttttcc attgccgcct cctggttctg
    13801 cacatttgtg caaatacgct ttctcaccgc ttgatctgat ataaccccat catgagggcc
    13861 tttcaaagcg atttttcact ctttgcatgg ggagaaatag cgctttctct tgcacatttg
    13921 tttctctgcg tagtagtcat cagcacaggt gtgcagcgat tcgtaatatg ttgattatcc
    13981 ttattttgcc aggcaaattt gcatcgtact tccggagaca cttttgtgat aaaaggcgtg
    14041 gcagatcaca tttaatttcg acgttgccgc ataaccctgt ttttccccgc taagaggtac
    14101 ggtatattcc gcgttttaca ggagaattta tgctgcccga ctcatcagtc cgtttaaata
    14161 aatacatcag cgaaagcgga atttgctcac gccgcgaagc ggatcgctat atcgagcaag
    14221 gcaatgtgtt ccttaatggc aagcgagcca ccattggcga tcaggtgaaa cccggcgaca
    14281 ttgtgaaagt aaacggtcag ttgattgagc ctcgggaagc cgaagatttg gtacttatcg
    14341 ccctgaacaa gcccgtcggt attgtcagca ccaccgaaga tggcgagcgc gataacattg
    14401 tcgatttcgt taaccacagc aaacgcgtgt tcccgattgg ccgcctggat aaagactccc
    14461 aggggctgat tttcctcacc aatcacggcg atctggtgaa taagatcctc cgtgcgggta
    14521 acgatcatga gaaagagtat ctggtgacgg tcgataaacc gattaccgat gaatttattc
    14581 gtggtatggg ggcgggggtg ccgatcctcg ggacggtgac gaaaaagtgc aaagtcaaaa
    14641 aagaagcgcc gtttgtcttc cgcattaccc tggtgcaggg gctgaaccgt cagatccggc
    14701 gcatgtgcga gcatttcggc tatgaagtga aaaagctgga acgtacgcgc attatgaacg
    14761 tcagtttaag cggcattccg ctgggtgagt ggcgcgattt aactgacgat gagttaatcg
    14821 acctgtttaa actcattgaa aattcctctt ccgaggtaaa acctaaagcg aaggctaaac
    14881 cgaaaacagc gggcatcaaa cgtccagtcg ttaagatgga aaaaacggcg gaaaaaggcg
    14941 gtcgcccggc gtccaacggt aagcgtttta cctcgccggg gcgtaaaaag aagggacgct
    15001 aatcagcgcc tgccgcgcgt cgacgtattt gctgaccagc taaatgacgc ttcaggatcc
    15061 ggtttataag cctgcttttt cttcaactgg cgggcttttt tcgcttcagc ctcacgctcg
    15121 accattaatt tatcgatgta ctctttctta atgctgttgg tttcggagtt ggtcagtacg
    15181 cgaccatgcg caatccgcgc ccgatccagc agtgttttaa gttcacgctg ctcgcgttca
    15241 gtcatctctt tttgggtaat acgggggagt gccatgatgg tgccctcagt gagccagaga
    15301 gttcagtgta cgggaattcg tttgcgagct gaactgataa tattcctttg ttctcttctt
    15361 aaatatttat ctttcttgat aattgacgat aaatacagat a
//
LOCUS       DAZFWL020000111        15297 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFWL020000111 DAZFWL020000000
VERSION     DAZFWL020000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.111"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(37..843)
                     /gene="trpA"
                     /locus_tag="ACHKNP_RS18250"
                     /old_locus_tag="pgaptmp_003650"
     CDS             complement(37..843)
                     /gene="trpA"
                     /locus_tag="ACHKNP_RS18250"
                     /old_locus_tag="pgaptmp_003650"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGU4705743.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(843..2036)
                     /gene="trpB"
                     /locus_tag="ACHKNP_RS18255"
                     /old_locus_tag="pgaptmp_003651"
     CDS             complement(843..2036)
                     /gene="trpB"
                     /locus_tag="ACHKNP_RS18255"
                     /old_locus_tag="pgaptmp_003651"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGU4705744.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2048..3409)
                     /gene="trpCF"
                     /locus_tag="ACHKNP_RS18260"
                     /old_locus_tag="pgaptmp_003652"
     CDS             complement(2048..3409)
                     /gene="trpCF"
                     /locus_tag="ACHKNP_RS18260"
                     /old_locus_tag="pgaptmp_003652"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HGU4705745.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKSSPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3410..5005)
                     /gene="trpD"
                     /locus_tag="ACHKNP_RS18265"
                     /old_locus_tag="pgaptmp_003653"
     CDS             complement(3410..5005)
                     /gene="trpD"
                     /locus_tag="ACHKNP_RS18265"
                     /old_locus_tag="pgaptmp_003653"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HGU4705746.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5005..6567)
                     /gene="trpE"
                     /locus_tag="ACHKNP_RS18270"
                     /old_locus_tag="pgaptmp_003654"
     CDS             complement(5005..6567)
                     /gene="trpE"
                     /locus_tag="ACHKNP_RS18270"
                     /old_locus_tag="pgaptmp_003654"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGU4705747.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGMENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNMLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6589..6682)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6659..6703)
                     /gene="trpL"
                     /locus_tag="ACHKNP_RS18275"
                     /old_locus_tag="pgaptmp_003655"
     CDS             complement(6659..6703)
                     /gene="trpL"
                     /locus_tag="ACHKNP_RS18275"
                     /old_locus_tag="pgaptmp_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HGU4705748.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6841..7722
                     /gene="rnm"
                     /locus_tag="ACHKNP_RS18280"
                     /old_locus_tag="pgaptmp_003656"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6841..7722
                     /gene="rnm"
                     /locus_tag="ACHKNP_RS18280"
                     /old_locus_tag="pgaptmp_003656"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HGU4705749.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7719..8339
                     /gene="yciO"
                     /locus_tag="ACHKNP_RS18285"
                     /old_locus_tag="pgaptmp_003657"
     CDS             7719..8339
                     /gene="yciO"
                     /locus_tag="ACHKNP_RS18285"
                     /old_locus_tag="pgaptmp_003657"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGU4705750.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDKPVVVREGVGDVKPFL"
     gene            8440..9312
                     /gene="rluB"
                     /locus_tag="ACHKNP_RS18290"
                     /old_locus_tag="pgaptmp_003658"
     CDS             8440..9312
                     /gene="rluB"
                     /locus_tag="ACHKNP_RS18290"
                     /old_locus_tag="pgaptmp_003658"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HGU4705751.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9352..9942)
                     /gene="cobO"
                     /locus_tag="ACHKNP_RS18295"
                     /old_locus_tag="pgaptmp_003659"
     CDS             complement(9352..9942)
                     /gene="cobO"
                     /locus_tag="ACHKNP_RS18295"
                     /old_locus_tag="pgaptmp_003659"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGU4705752.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(9939..10697)
                     /gene="yciK"
                     /locus_tag="ACHKNP_RS18300"
                     /old_locus_tag="pgaptmp_003660"
     CDS             complement(9939..10697)
                     /gene="yciK"
                     /locus_tag="ACHKNP_RS18300"
                     /old_locus_tag="pgaptmp_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HGU4705753.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFLLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            10917..11966
                     /gene="sohB"
                     /locus_tag="ACHKNP_RS18305"
                     /old_locus_tag="pgaptmp_003661"
     CDS             10917..11966
                     /gene="sohB"
                     /locus_tag="ACHKNP_RS18305"
                     /old_locus_tag="pgaptmp_003661"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HGU4705754.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12002..12253)
                     /gene="yciN"
                     /locus_tag="ACHKNP_RS18310"
                     /old_locus_tag="pgaptmp_003662"
     CDS             complement(12002..12253)
                     /gene="yciN"
                     /locus_tag="ACHKNP_RS18310"
                     /old_locus_tag="pgaptmp_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HGU4705755.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12633..15230
                     /gene="topA"
                     /locus_tag="ACHKNP_RS18315"
                     /old_locus_tag="pgaptmp_003663"
     CDS             12633..15230
                     /gene="topA"
                     /locus_tag="ACHKNP_RS18315"
                     /old_locus_tag="pgaptmp_003663"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGU4705756.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         3706 a         3908 c         4104 g         3579 t
ORIGIN      
        1 gttaaaatgc cgccagcgga actggcggct gtgggattaa ctgcgcgtcg ccgctttcat
       61 cggttgtaca aaaactttca gtgccgccag cattttctct ggctcattaa tatgttgctc
      121 gatgatttta acaatggccg aaccagaaat cgcgcccgca gctcctgcat caatcgctgc
      181 ttttacctga tccggggcgg aaataccaaa tccctgcaat ggaggtgcag cgttgtactc
      241 tttcagcttc gcaaccagat gattgagggg taacgcggcg cggttttctg cgccggtcac
      301 gcctgctcgt gacagcaaat aggtgtaacc acgaccgtaa gaggctatct ggcgcagcag
      361 gtcgtcatcg gcatttggcg ggcagatgaa gataggtgcg acattatgac gcaacgcggc
      421 ctggcggaag ggcgcggact cttcaactgg cacatcggca accagcaccg aatcgacgcc
      481 gactttttcg cactgggcat aaaactcatc aatgcctttg ttaaacacca gattggcata
      541 catcaacagg ccaatgggaa tggtcgggtg tttctggcga atcagtgcca gcatttcaaa
      601 acattgtgcc ggagtcacac ctgccgcaaa ggcgcgcaga gtggcgtttt gaatcgtcgg
      661 gccatccgcc agtgggtcgg agaaggggat acctaactcc agcgcgtcag caccggcttc
      721 aattagcgta tcgataattt tcaatgactg ctcaatgccc ggatcaccga gcgtgacgaa
      781 aggaacgaat gcgccttctt tgcgctcctt caactgggca aacagagatt cgtagcgttc
      841 catcagattt cccctcgtgc tttcaaaata tcgtgaacgg tgaagatgtc tttatcgccg
      901 cgaccggaaa ggttaaccac cagtagctgc tctttatccg ggttttcgcg catcattttc
      961 aacgcatggg ccagggcgtg ggaggattcc agcgccggga tgatcccttc gtgcaggcac
     1021 agcgttttga aggcttcaag ggcttcatca tcggtaatag acacgtaatc agcgcgtcca
     1081 gtgctgttaa gatacgcgtg ttgtgggccg acagacggga aatccagtcc ggcggagatg
     1141 gagtaagatt cttcaatctg cccgtcttcg gtttgcatca tcggcgcttt cataccgaaa
     1201 tagataccca cgcgaccatg ttttagcggt gcgccgtgct cgccagtttc gataccgtga
     1261 ccacctggct ccacaccaat caggccgacg ttggtttcat tgatgaaatc agcaaacatg
     1321 ccgatggcat tcgaaccgcc gccaacacag gcgataacgg catccggcag gcgaccttct
     1381 ctttccagaa tctgcgcttt ggtttcttcg ccaatcatcc gctgaaactc acgcacaatg
     1441 gtcggataag gatgcgggcc agctgcggtg cccagcatat agtgcgcggt ttcgtaacta
     1501 ccggaccagt cgcgcagcgc ctcgttacag gcatctttca gcgtcgcgga accgctatgc
     1561 accgggatca cttccgcacc cattaagcgc atacgaaaaa cgttaggcga ctggcgttca
     1621 acgtctttgg cacccatata aatacggcat ttcaggccga gcagggcgct ggcaagggcc
     1681 gacgccacgc catgctgacc ggcaccggtt tcggcgatga tttcggtttt acccatccgc
     1741 ttcgccagca acgcctgccc cagcacctgg ttagttttat gcgcgccgcc gtgcagcaaa
     1801 tcttcacgct tgagatacag cgtagtgttc gtcccggctg taatgttctg gcatttggtc
     1861 agcgcggttg gacgcccggc atagtttttc agcaggtcgt tgaactgagc ctgaaattca
     1921 ggatcttttt gcgcactgac aaaagcttct tccagctggc gcagagcagg catcaggatt
     1981 tgtggcacgt acatgccgcc aaactcacca aaataggggt taagtaatgt tgtcattgtt
     2041 cctttcctta atatgcgcgc agcgtctgga aaaccgaggc caaaagacgt gcgtctttga
     2101 tgcccggttg cgactctaca gcagaattaa aatcaagtcc ggcgcagccg gtttgtgccg
     2161 cttccacgca gttatctgcg cctaagcccc ccgccagcag aacgttgcca agcgattgac
     2221 catttaatag tgaccagtca aaacgttgcc cgcttccacc ctggccgttg tctaaaacat
     2281 atttatcaac gtgctgaaac tcgcgggcgg gcagggtttc accgacgctt aatgctttcc
     2341 agatggcaac atgtgctggc agagcttcac gcagcgtatc gatatacagc tgttcttcat
     2401 taccatgcag ttgcactgcc gccagcgata acaccttagc tttgtccacc acatcggcaa
     2461 tatcgtgatt gcggaacacg ccaacatact gcaacggtgc cgcagccatc acttcctgcg
     2521 cctgttcaac gttgacgcaa cgcggtgatg tcgcaacaaa aatcaaccca ccgtaaatcg
     2581 cgcccgcgtc ataagctgct ttagcatctt gcccacgcgt caggccacat actttattct
     2641 cacccagcaa cacccggcgc acggcggcgt gcaaatcgtc atgggccatc aacgccgaac
     2701 caatcagaaa accgttagcg aagtggctta actcgcgcac ctgagcgtaa gtattgatgc
     2761 cggattcgct gattaccgtc acgttgtgcc ccagtttcgg cgcaagctcg cgggtacggt
     2821 tgagatcaat cgacaaatca cgcagatcgc ggttgttgat gccaacgacc tttgccccca
     2881 atgcaatggc acgctccagt tcctcttcat tactgacttc ggtcagcaca cccatctcca
     2941 gactgtgggc gacggcggca agctggcgat attgttcgtc atccagtact gaaagcatta
     3001 ataagcaggc atcagcctgg taatagcgcg ccagatagat ctggtaagga tcgataatga
     3061 agtctttaca taaaatcggc tgcggggcga tttggctgac gatggggagg aaatcaaagc
     3121 tcccctgaaa atatttctca tcagtcagca ccgaaattgc cgaagcgtaa tgtttataaa
     3181 tggcggcaat gcgtgctgga tcgaaatcat cacggatcac gccttttgac ggcgacgatt
     3241 tcttgcactc cagaataaac gccgtgcgcg caccctgtag cgcatcataa aaatgtcgcg
     3301 tgctcggctg aacctcattc tgaaaactgg ccagcggttg ctgctgtttg cgggtttcta
     3361 cccaaatcgc cttgtctgcg acgattttcg ctaaaacggt ttgcatcatt taccctcgtg
     3421 ccgccagtgc ggtaactctg tcgtaagcgg aaccactgcg cagtacctca agaacggttt
     3481 gcgcattggc ttgcagatct tcatggccat gcaggcgcat taacatggcg acgttcgcag
     3541 cgacggctgc ttcatgggcg gcgtcgcctt taccttgtaa caagcgtgtt aaaatgtcac
     3601 ggttttcttc cggtgttccg cctgccagtt gctcctggtg gtagggagtc aggccaaaat
     3661 cttcagcggt caattgatag ctcttaattt cgccgtcatg cagctcggca acgattgtcg
     3721 gcgcgtgtaa tgaaacttca tccatcccgc cgctgtgcac caccgccgcg cgttgatacc
     3781 ccagcacgcg caaggtttcg gcaatcggca gcaccagttc cggactataa acaccaatta
     3841 acgccagcgg cggatgcgcc gggttaatca atggccccag cacattgaac agggtgcggg
     3901 ttttcagttg ctggcgaacc ggcatcgcgt ggcggaatcc ggtgtgatac ttcggcgcaa
     3961 agaggaaaca tacacctaac tcatccagcg cctggcgcga tttatcggcg ttcatatcaa
     4021 gattaatacc gaacgccgcc agcagatcgg acgaaccaga tttactggag acgctacggt
     4081 tgccgtgttt cgccactttc agcccacagg ccgcggcgac aaacgcactg gcggtagaaa
     4141 tattgatact gttgctgccg tcaccgccag taccgacgat atcagcaaac agataatccg
     4201 ggcgcgggaa cggcgcggcg ttttccagta gcgcggttgc tgctccggcg atctcgttcg
     4261 ggtgctcacc gcgaattttc atgctcacca gcgccgccgc cagttgttcc ggcttcagct
     4321 cgccacgcac caccgctgaa aacagctggt ggctttcttg ttggctaagc gtctgcgcct
     4381 gatacagttt ttccagaatc ggttgcagcg tgttggctgg ctctagtttc tgctgcgccc
     4441 aggccagcgt ttgttccagc aggcgagcgc cctgggtggt gagaatggat tccggatgga
     4501 actggaatcc acaaacgcga tccgcatcgt gacgtactgc catcaccatg ccattaaaat
     4561 gggcgttgat ggttaaaccg gccggaatgt tactgccaac cagcgagtga taacgcgcca
     4621 ccggcagcgg gtttgttaat ccggcaaaca tcgcctgacc gtcatgttca atgctcgacg
     4681 ctttaccgtg aagaatttcg cccgcctgac cgacatagcc cccgtaagct tcgacaattg
     4741 cctgatgtcc gaggcaaatg ccaataatgg gcagcttgcc acgcaagcgg gtgaggagtt
     4801 ccggcataca accggcttcg ctcggcacac cggggccagg agaaagcatc agtaccggat
     4861 tgctcatcgt cgccaggcgt tcaattaagg tctgcgccgg aatatggttg cggtaaatca
     4921 ccacattatg cccattgctg cgcaactgat ctgccaggtt gtacgtaaaa gagtcgatat
     4981 tatcgagcag cagaatgtca gccatcagaa agtctcctgt gcatgatgcg cggtggcaat
     5041 agcgcgcagt acagcgcggg ctttattacg ggtttcgtcg gcttccgact gcggaacaga
     5101 atcaaggact acgccagcac cggcttgcac ggtggcgata ccgttttcca ccagcgccga
     5161 gcggatcaca atgcaggtgt cgagatcgcc atgcgcggtg aaataaccta ccgcgccgcc
     5221 gtagctgccg cggcgacgac cttccgcctc ggcaattaac tgcatagcgc gtactttcgg
     5281 cgcaccactt aacgttccca tattcataca ggcgcggtaa gcgtgcaggg cgtcgagatc
     5341 gtggcgcagc tcaccgacca cgcgggagac caagtgcatc acgtaagagt aacggtcaac
     5401 tttggtgagg tcggcgacgt agcggctgcc gggggtgcaa atgcgtgcca gatcattacg
     5461 ggcgagatcc accagcatca gatgttcaga aagctcttta tgatcggtac gcatttccag
     5521 ttcgatgcgg ctgtcgaggt ctctgtccag tgaaccatcg gcacgacgac cgcgcggtcg
     5581 tgttccggca atcgggtaga tctcaatctg gcggctggtg gcgtcatact tgagcgaact
     5641 ttccggcgac gcgccaaaca gggtgaaatc attatcctgc ataaaaaaca tgtacgggct
     5701 gggattactc tttttcagca cgtaataggc cgccagcggt gacgggcagg gcagagagaa
     5761 acggcgagat ggcaccacct ggaaaatttc tccggcgcga atcgcttttt gcaacaaacg
     5821 cactacgcca ccgaactctt catcgctctg gttacattca caacgcatat gcggcacgga
     5881 aaccaccggc agcggtggcg cggtttcggt cagttgctgg cgaagatcgt tcaggcgagc
     5941 agtgagacgt tgtttttctt cttcattcgg agcaaacagg ctggcctgaa tgcgggtgct
     6001 ttttttctga tggtcaatca ccatcagcgt ttcagcgaga taaaaacaga aatcaggaca
     6061 gttattttcc gctgacaatt gcggtaaatc ttcaaacccc gccacaaggt cataagagaa
     6121 caggccgccg aagaacattg cttctcgttc ttccttcggt acattcagca tattctgcaa
     6181 taagcggaaa gcgtcaaaaa ccgaaaggga gcataagcga gcgtcttcat ccagcagtgg
     6241 actgacaggg gggaagcgca gcacacggca gtttggtaat tgttcatttt ccatacccgc
     6301 aggcagggcg ttatccagta gtgtcagcag ggcttcgccg ttgccggaaa gtgcctggat
     6361 tgtgacagtg tcacctaaag ctgtaatgcg cagcgcactg tctaccagca gcaggctttt
     6421 taaatcatct ttgctgtcga tatctgcgga ttccagcagc agcgttgccg gacgatcccc
     6481 acacaactgg tgaaaaagcg cggtgggatt gtcgcgataa gcgccttcgc aggttagcag
     6541 ttcgagagtc ggtttttgtg tttgcattgt tattctctaa ttttgttcaa aaaaaagccc
     6601 gctcattagg cgggctgggt atctgattgc tttacgcatg gtgaatacac tgcccgtttc
     6661 aggaagtgcg ccaccaacct ttcagtacga aaattgcttt cattgtcgat acccttttta
     6721 cgtgaacttg cgtactagtt aactagttcg atgattaatt gtcaatagct catttcagaa
     6781 tatttgccag aaccgttatg atgtcggcgc aaaaaacatt atccagaacg ggagtgcgcc
     6841 ttgagcgaca cgaattatgc agtgatttac gacctgcaca gccataccac agcttccgat
     6901 ggctgcctga cgccagaagc attggtgcac cgtgcagtcg agatgcgcgt cggcaccctg
     6961 gcgatcaccg accatgacac cacagctgca atcgcgccag caagagagga aatttcacgt
     7021 tcgggactgg cgttgaatct tattcccggc gtggaaattt ccacggtctg ggaaaatcat
     7081 gaaattcata ttgtcgggct gaatattgat attactcatc cgctaatgtg tgagttcctc
     7141 gcgcagcaga cagaacggcg caatcagcgg gcgcagctga ttgccgaaag acttgaaaaa
     7201 gcgcaaatac ctggggcgct ggaaggcgca caacgactgg cgcagggcgg cgcagtgacg
     7261 cgcggtcatt ttgcgcgttt ccttgttgag tgcggcaaag ccagttcaat ggcggatgtc
     7321 tttaaaaagt atctggcgcg cgggaaaacc ggatacgttc cgccacagtg gtgtacaata
     7381 gaacaagcta ttgatgtcat tcatcattct ggcggtaagg cggtgctagc tcatcctggg
     7441 cggtacaatc ttagtgctaa atggctgaaa agattggttg cgcattttgc cgaacaccac
     7501 ggtgacgcga tggaagtcgc gcagtgccag caatcgccca atgaacgtac ccagctggcg
     7561 gcccttgcgc gtcagcatca tttatgggca tcacaaggat ctgattttca tcagccatgc
     7621 ccgtggatcg aactgggtcg taaactctgg ctgcccgcag gcgttgaagg cgtctggcag
     7681 ctatgggaac agccgcagaa caccacagag agggaattat gagccagttt ttttatattc
     7741 atcctgataa cccacagcaa cgtctgatca accaggcggt ggagatcgtg cgtaaaggcg
     7801 gggtgattgt ttatccaact gattccggct atgcgctcgg ctgtaaaatt gaagataaaa
     7861 acgcgatgga gcgtatttgt cgtattcgcc agctgccgga cggtcacaac tttaccctga
     7921 tgtgtcgcga tctttctgaa ctctcgacct attcatttgt tgataacgtt gcgtttcgtt
     7981 taatgaaaaa caacacgccg ggcaactata ccttcatctt gaaggggacg aaggaagtgc
     8041 cacgccgtct gttgcaggaa aaacgcaaaa ccatcggtat gcgtgtgcct tctaacccga
     8101 ttgcgcaggc gttgcttgaa gcactgggcg aaccgatgct ttccacttcg ctaatgctgc
     8161 caggcagcga atttaccgaa tccgatcccg aagaaattaa agaccgtctg gaaaaacagg
     8221 tagatttgat tattcacggc ggctatctcg ggcagaaacc gacaacggtt attgatctta
     8281 ccgatgataa gccggtcgtg gtgcgtgaag gcgtaggtga tgtgaagcct ttcttataag
     8341 tgcgtaactc tgtatactac gcggccttaa aatggcgcta tacggcgcca ttgattctcc
     8401 tgttcgacgc ctgtgaaggc gacactaaaa ggaagctcta tgagcgaaaa attacagaaa
     8461 gtgctggccc gcgctggcca cggctcccgc cgtgaaatcg aatctattat tgaagccggt
     8521 cgtgtgagtg ttgatggcaa aattgctaaa ctcggcgatc gcgttgaagt tacccctggg
     8581 ctgaaaattc gtatcgatgg tcatctgatt tcggtacgtg agtccgctga acaaatttgt
     8641 cgcgttctgg cctattacaa accggaaggt gagttgtgta cccgtaacga tccggaaggg
     8701 cgtccgacgg tgtttgaccg tctgccaaag ctgcgtggcg cacgctggat tgccgtaggt
     8761 cgtctggacg ttaacacctg tggtctgctg ctgttcacca ccgatggtga actggcaaac
     8821 cgtttaatgc atccaagccg tgaagttgaa cgtgaatacg ccgtgcgtgt atttggtcag
     8881 gttgacgacg cgaaactgcg tgatttgagt cgtggcgtgc aactggaaga tggtccggca
     8941 gcttttaaaa ccatcaagtt cagcggcggc gaagggatca accagtggta caacgtgact
     9001 ctgaccgaag gccgtaaccg tgaagttcgt cgtctgtggg aagcggttgg tgtgcaggtg
     9061 agccgcctga tccgtgttcg ttacggtgat atcccactgc caaaaggtct gccacgcggt
     9121 ggctggacgg agctggacct cgcccagact aactatctgc gcgaactggt ggagctaccg
     9181 ccagaaacca gctctaaagt cgctgtagaa aaagaccgtc gtcgcatgaa ggcgaatcag
     9241 attcgtcgtg cggtgaaacg tcacagtcag gtgagcggcg gtcgtcgttc tggcggacgt
     9301 aataacggtt aatcaaagta tgccggacgt catatccggc atttttacag attagtaatc
     9361 aatccctatc tgcgctttta caccggcatc aaacgcatgt ttgacggggc gtaattcact
     9421 taccgtatct gccagttcaa gaatatcccg atgacaacca cgacccgtga taatcaccgt
     9481 ctgttgatgt ggacgttcat ttaacgcctg caccacttct tccagtggca aatagtcata
     9541 cgccaccata tacgtcagtt catcaagcaa aaccatatcc agtgaggaat cagcaagcat
     9601 ccgctttgca tgttgccaga cttcgcggca ggcggcggta tcagactcgc ggttttgtgt
     9661 atcccaggta aagcccgttg ccatcacctg aaactcaacg ccatgtggct ccagcagatt
     9721 gcgttcgcca ttaggccagg tgcctttaat aaactgcacg acacctactt tttttccgtg
     9781 accaactgcg cgtgttgccg taccaaatgc cgcggtggtt ttgccttttc cattgccggt
     9841 aaagacgatg ataataccgc gttcatcctg ggcctgggcc acacgagcat ctactttttc
     9901 tttcactcgc tgctgacgct gttggtagcg ttcatcactc attgggaaat tcctggttta
     9961 cggcccggtt gggcgtcaaa ggtcatgccg gttttacggc ggctgtcatc gcccatcagc
    10021 cagaggtaga gcggcatgat atcagcgggt gttttaagtt tctgtggatc ttcggtcggg
    10081 aaggcgctgg cacgcattgc ggtgcgcgta ccgcctgggt taatgcagtt gacgcgcagg
    10141 cgctgctgat attcatcggc cagtacctgc atcatccctt cggtggcaaa tttcgacgct
    10201 gcatatgcac cccagttagc tcgtccctga cgtccaacgc ttgatgaagt aaagaccagt
    10261 gaaccggcgt ccgatttgag taataaagga agcagcgcct gggtgagcaa aaaggtggca
    10321 ttaacgttga cctgcatgac gtcctgccag acctgcggat tttgttcgct cattgggcaa
    10381 acatcgccga gcaatccggc attatgcaaa acaccatcca gacgcggata attaacggta
    10441 atgcgctgtg ccagttgttg gcaatcttcg gacgtgcagg tcagcaaatc gagaataaac
    10501 cactgtggct gacgtccagt ttcttcgttt atgtggctgg ctacctgacg taatttttct
    10561 tcattacggc ccaacagaat cactgtcgca ccatagcgtg catacgtcat cgcggcttca
    10621 cggccaatcc catcgctggc tcccgtcacc aggataatgc gatcattgag taaatcttgt
    10681 tttggctggt aatgcatggc tactcctcaa cgacgttgtc tgtcgcgctg ccagtgtact
    10741 ttatgacttt ggggctttat gcctgaaaca gaagggcatt tcaatcagcc tggggcgata
    10801 acaccgcaaa atggatactt tgtcatactt tcgctgcaat aacatctctg cgagacggct
    10861 taacatgcct gttgtaaact gtgagccaaa gcgttgttta accaaggtgg ggactcgtgg
    10921 aattgttgtc tgaatatggt ttgtttttgg cgaaaatcgt taccgttgtg ctagcgattg
    10981 cggcgattgc cgccattatt gtcaatgttg ctcaacgtaa taaacgccag cgtggcgagt
    11041 tacgggtcaa caatctcagc gaacagtata aggagatgaa agaagaactg gccgcggcgc
    11101 tgatggactc acatcagcaa aaacagtggc acaaagcgca gaagaaaaag cacaagcaag
    11161 aagcgaaagc agcaaaagcg aaagccaaac tgggcgaggt ggcaactgac agtaaacctc
    11221 gcgtctgggt gctggatttt aaaggcagca tggacgccca tgaagtgaac tcgctacgtg
    11281 aagagataac ggcggtactc gcagcattca aaccgcagga tcaggttgtg ctccgtctgg
    11341 aaagccctgg tggcatggtg catggttacg ggttggcggc ttcgcagctg cagcgtctgc
    11401 gtgataaaaa cattccttta actgttacgg tagacaaagt cgctgccagc ggcggttaca
    11461 tgatggcctg tgtggcggac aaaattgttt ccgcaccgtt tgctattgtg ggttccattg
    11521 gggtggtggc gcaaatgccc aactttaacc gcttcctgaa aagcaaagat attgatatcg
    11581 aactgcacac tgccgggcag tataagcgta ctctgacctt gctgggtgaa aataccgaag
    11641 aagggcggga gaaattccgc gaagagttga acgaaacgca tcagttgttt aaagattttg
    11701 tgaagcgtat gcgtccgtct ctggatattg aacaggtggc aacgggtgaa cactggtacg
    11761 gacaacaggc ggtagagaaa ggcctggttg atgaaatcaa caccagtgat gaagttattc
    11821 ttagcctgat ggaaggccgt gaagtggtta atgtacgcta tatgcagcgt aaacgactca
    11881 ttgaccgatt caccggcagc gcggcagaga gcgccgatcg attgttgcta cgctggtggc
    11941 agcggggtca aaagccattg atgtaaaaga caaacgcgag gctaagacct cgcgttttgc
    12001 tttaatcaac cagatgatat ttttctgaaa gcacatgggc caggtgtttg aacatattaa
    12061 acaccgcggt gcttttggct gttggcaatc cttgttcatc taaaaagtag tcgccggtaa
    12121 ataccagcac gccattacgc tgcgtgacgc cggtggcttc aatccctgcg agcgtatcct
    12181 catgctcacg aatgattttg ttggcctctt tcaacagcgt ttcgcggtcg atgggttgtg
    12241 tctctttgtt cattatttac tccttaaaca aggacattag tctacgccag gcatggcttg
    12301 cagacaaata taccacgctg gtggcaagag cgccttactg gtaactttgg attttgcatg
    12361 ctaataaagt tgcgtatcgg attttatcag gtacagtgtg acgctttcgt caatctggca
    12421 atagatttgc ttgacattcg accaaaattc cgtcgtgcta tagcgcctgt aggccaagac
    12481 ctgttaactc agtcacctga attttcgtga acagagtcac gacaaggggt tgatatccgc
    12541 agagaacgag tccatatcgg taactcgttg ccagtggaag gtttatcaac gtgcgacgca
    12601 ttcctggaag aatcaaatta ggtaaggtga atatgggtaa agctcttgtc atcgttgagt
    12661 ccccggcaaa agccaaaacg atcaacaagt atctgggtag tgactacgtg gtgaaatcca
    12721 gcgtcggtca catccgcgat ttgccgacca gtggctcagc tgccaaaaag agtgccgact
    12781 ctacctccac caagacggct aaaaagccta aaaaggatga acgtggcgct ctcgtcaacc
    12841 gtatgggggt tgacccgtgg cacaattggg aggcgcacta tgaagtgttg cctggtaaag
    12901 agaaggtcgt ctctgaactg aaacaactgg ctgaaaaagc cgaccacatc tatctcgcaa
    12961 ccgaccttga ccgcgaaggg gaagccattg catggcacct gcgggaagtg attgggggtg
    13021 atgatgcgcg ctatagccga gtggtgttta acgaaattac taaaaacgcg atccgccagg
    13081 catttaataa accgggtgag ctgaatattg atcgtgttaa tgcccagcag gcgcgtcgct
    13141 ttatggaccg cgtggtgggg tatatggttt cgccgctgct atggaaaaag atcgctcgtg
    13201 gtctgtctgc cggtcgtgtg cagtcggtgg cggttcgcct ggtggtcgag cgtgagcgtg
    13261 aaattaaagc gttcgtgccg gaagagttct gggaagtcga tgccagcacg accacgccat
    13321 ctggtgaagc gttggcgtta caggtgactc atcagaacga caaaccgttc cgtccggtca
    13381 acaaagaaca aactcaggct gcggtaagtc tgctggaaaa agcgcgctac agcgtgctgg
    13441 aacgtgaaga caaaccgaca accagtaaac ctggcgctcc ttttattacc tctacgctgc
    13501 aacaagctgc cagcacccgt cttggatttg gcgtgaaaaa aaccatgatg atggcgcagc
    13561 gtttgtatga agcaggctat atcacttaca tgcgtaccga ctccactaac ctgagtcagg
    13621 acgcggtaaa tatggttcgc ggttatatca gcgataattt tggtaagaaa tatctgccgg
    13681 aaagtccgaa tcagtacgcc agcaaagaaa actcacagga agcgcacgaa gcgattcgtc
    13741 cttctgacgt caatgtgatg gcggaatcgc tgaaggatat ggaagcagat gcgcagaaac
    13801 tgtaccagtt aatctggcgt cagttcgtag cctgccagat gaccccagcg aaatatgact
    13861 ccacgacgct gaccgttggt gcgggcgatt tccgcctgaa agcacgcggt cgtattttgc
    13921 gttttgatgg ctggacaaaa gtgatgcctg cgttgcgtaa aggcgatgaa gatcgcatct
    13981 taccagcagt taataaaggc gatgctctga cgctcgttga acttacacca gcccagcact
    14041 ttaccaagcc gccagcccgt ttcagtgaag catcgctggt taaagagctg gaaaaacgtg
    14101 gtatcggtcg tccgtctact tatgcgtcga tcatttcgac cattcaggat cgtggctacg
    14161 tgcgagtaga aaatcgtcgt ttctatgcgg aaaaaatggg cgaaatcgtc accgatcgcc
    14221 ttgaagaaaa tttccgcgag ttaatgaact acgactttac cgcgcagatg gaaaacagcc
    14281 tcgaccaggt ggcaaatcac gaagcagagt ggaaagctgt actggatcac ttcttctcgg
    14341 atttcaccca gcagttagat aaagctgaaa aagatccgga agagggtggt atgcgcccga
    14401 accagatggt tctgaccagc attgactgcc cgacttgtgg tcgcaaaatg gggattcgca
    14461 cagcgagcac cggggtattc cttggctgtt ctggctatgc gctgccgccg aaagagcgtt
    14521 gcaaaaccac cattaacctg gtgccggaaa acgaagtgct gaacgtgctg gaaggcgaag
    14581 atgctgaaac caacgcgctg cgcgcaaaac gtcgttgccc gaaatgcggc acggcgatgg
    14641 acagctatct catcgatccg aaacgtaagt tgcatgtctg tggtaataac ccaacctgcg
    14701 acggttacga gatcgaagag ggcgaattcc gcattaaagg ttatgacggc ccgatcgttg
    14761 agtgtgaaaa atgtggctct gaaatgcacc tgaaaatggg gcgtttcggt aaatacatgg
    14821 cctgcaccaa cgaagagtgt aaaaacacac gtaagatttt acgtaacggc gaagtggcac
    14881 caccgaaaga agatccggtg ccattacctg agctgccgtg cgaaaaatca gatgcttatt
    14941 tcgtgctgcg tgacggtgct gccggtgtgt tcctggctgc caacactttc ccgaaatcgc
    15001 gtgaaacgcg tgcgccactg gtggaagagc tttatcgctt ccgcgaccgt ctgccggaaa
    15061 aactgcgtta tctggccgat gcgccacagc aggatccgga aggtaataag accatggttc
    15121 gctttagccg taaaaccaaa cagcaatatg tctcttcgga aaaagacgga aaggcgactg
    15181 gctggtcagc attttatgtt gatggcaaat gggttgaagg aaaaaaataa cctttaattc
    15241 tgtcaggttt ttataaacaa agggtcgcga aagcggccct tttttattgc atattat
//
LOCUS       DAZFWL020000112        15218 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFWL020000112 DAZFWL020000000
VERSION     DAZFWL020000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.112"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            77..1054
                     /gene="ybeQ"
                     /locus_tag="ACHKNP_RS18320"
                     /old_locus_tag="pgaptmp_003664"
     CDS             77..1054
                     /gene="ybeQ"
                     /locus_tag="ACHKNP_RS18320"
                     /old_locus_tag="pgaptmp_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HGU4705757.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKVNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1124..1606)
                     /gene="ybeL"
                     /locus_tag="ACHKNP_RS18325"
                     /old_locus_tag="pgaptmp_003665"
     CDS             complement(1124..1606)
                     /gene="ybeL"
                     /locus_tag="ACHKNP_RS18325"
                     /old_locus_tag="pgaptmp_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HGU4705758.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1841..4423
                     /gene="leuS"
                     /locus_tag="ACHKNP_RS18330"
                     /old_locus_tag="pgaptmp_003666"
     CDS             1841..4423
                     /gene="leuS"
                     /locus_tag="ACHKNP_RS18330"
                     /old_locus_tag="pgaptmp_003666"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGU4705759.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4438..5019
                     /gene="lptE"
                     /locus_tag="ACHKNP_RS18335"
                     /old_locus_tag="pgaptmp_003667"
     CDS             4438..5019
                     /gene="lptE"
                     /locus_tag="ACHKNP_RS18335"
                     /old_locus_tag="pgaptmp_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HGU4705760.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5019..6050
                     /gene="holA"
                     /locus_tag="ACHKNP_RS18340"
                     /old_locus_tag="pgaptmp_003668"
     CDS             5019..6050
                     /gene="holA"
                     /locus_tag="ACHKNP_RS18340"
                     /old_locus_tag="pgaptmp_003668"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGU4705761.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6052..6693
                     /gene="nadD"
                     /locus_tag="ACHKNP_RS18345"
                     /old_locus_tag="pgaptmp_003669"
     CDS             6052..6693
                     /gene="nadD"
                     /locus_tag="ACHKNP_RS18345"
                     /old_locus_tag="pgaptmp_003669"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGU4705762.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6717..7328
                     /gene="cobC"
                     /locus_tag="ACHKNP_RS18350"
                     /old_locus_tag="pgaptmp_003670"
     CDS             6717..7328
                     /gene="cobC"
                     /locus_tag="ACHKNP_RS18350"
                     /old_locus_tag="pgaptmp_003670"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HGU4705763.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7588..7905
                     /gene="rsfS"
                     /locus_tag="ACHKNP_RS18355"
                     /old_locus_tag="pgaptmp_003671"
     CDS             7588..7905
                     /gene="rsfS"
                     /locus_tag="ACHKNP_RS18355"
                     /old_locus_tag="pgaptmp_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGU4705764.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7909..8376
                     /gene="rlmH"
                     /locus_tag="ACHKNP_RS18360"
                     /old_locus_tag="pgaptmp_003672"
     CDS             7909..8376
                     /gene="rlmH"
                     /locus_tag="ACHKNP_RS18360"
                     /old_locus_tag="pgaptmp_003672"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGU4705765.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8407..10308
                     /gene="mrdA"
                     /locus_tag="ACHKNP_RS18365"
                     /old_locus_tag="pgaptmp_003673"
     CDS             8407..10308
                     /gene="mrdA"
                     /locus_tag="ACHKNP_RS18365"
                     /old_locus_tag="pgaptmp_003673"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HGU4705766.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10311..11423
                     /gene="mrdB"
                     /locus_tag="ACHKNP_RS18370"
                     /old_locus_tag="pgaptmp_003674"
                     /gene_synonym="rodA"
     CDS             10311..11423
                     /gene="mrdB"
                     /locus_tag="ACHKNP_RS18370"
                     /old_locus_tag="pgaptmp_003674"
                     /gene_synonym="rodA"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HGU4705767.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11434..12522
                     /gene="rlpA"
                     /locus_tag="ACHKNP_RS18375"
                     /old_locus_tag="pgaptmp_003675"
     CDS             11434..12522
                     /gene="rlpA"
                     /locus_tag="ACHKNP_RS18375"
                     /old_locus_tag="pgaptmp_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HGU4705768.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12662..13873
                     /gene="dacA"
                     /locus_tag="ACHKNP_RS18380"
                     /old_locus_tag="pgaptmp_003676"
     CDS             12662..13873
                     /gene="dacA"
                     /locus_tag="ACHKNP_RS18380"
                     /old_locus_tag="pgaptmp_003676"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HGU4705769.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13984..14247
                     /gene="ybeD"
                     /locus_tag="ACHKNP_RS18385"
                     /old_locus_tag="pgaptmp_003677"
     CDS             13984..14247
                     /gene="ybeD"
                     /locus_tag="ACHKNP_RS18385"
                     /old_locus_tag="pgaptmp_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HGU4705770.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14348..14989
                     /gene="lipB"
                     /locus_tag="ACHKNP_RS18390"
                     /old_locus_tag="pgaptmp_003678"
     CDS             14348..14989
                     /gene="lipB"
                     /locus_tag="ACHKNP_RS18390"
                     /old_locus_tag="pgaptmp_003678"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HGU4705771.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3779 a         3853 c         3956 g         3630 t
ORIGIN      
        1 cataaaataa ggcttacgca aaaatggaac acataaaaat ataatatgtt ttatctgcaa
       61 ccataaggaa atgataatga ttttcacgtc aagttgctgc gataatttat caatagatga
      121 gattatcgaa cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg ggttttatta
      181 taatcgcgat agcgcaattg attctccgga cgacgaaaaa gccttttact ggctgaagct
      241 ggccgctgag caaggtcatt gtgaagcaca gtattcctta gggcaaaagt ataccgagga
      301 taaaagccgt cataaagtta atgagcaagc catcttctgg ctgaaaaaag ctgccctaca
      361 aggccatact ttcgcttcca acgcccttgg ctggacactg gatcgtggag aagcccccaa
      421 ttataaagaa gcggttgtct ggtatcagat agccgcggag agcggaatgt cttatgcgca
      481 aaataatctt gggtggatgt acagaaatgg caacggagtc gcaaaagact atgcgctggc
      541 atttttctgg tacaaacaag ctgcattaca aggccatagt gacgcgcaaa acaatctggc
      601 cgatctttat gaagacggaa aaggcgttgc tcaaaacaag acactcgccg cattctggta
      661 tttgaaaagc gcacagcagg gtaatcggca cgcccagttt caaattgcat gggattataa
      721 cgctggcgaa ggggtggacc aggactataa gcaagcgatg tactggtatc tgaaggctgc
      781 cgctcaggaa agcgtcggcg cttacgtcaa catcggttat atgtataaac acggacaagg
      841 cgttgagaaa gattatcagg ctgcctttga atggtttacg aaagccgctg aatgcaatga
      901 cgccactgcc tggtataacc tggccattat gtatcactac ggagaaggaa gacctgtcga
      961 tctccgacag gctctcgacc tgtatcgtaa agttcagtca tccggaacca gagatgtcag
     1021 tcaggaaatt cgtgagactg aagatttact gtagagataa aaacaagggc cggggaattt
     1081 cattcggccc atattctctt tattgctgtg cgaaatcaga ctattacggc tcaaacgggc
     1141 gtctctggaa ctggtcatga ccacatttcg ggcatagcgt cagcacttcc ggtgtgtaga
     1201 tcgggagatg gaagtgacat ttctcgcaga ccagatttcc cagcccgacc acttctccgc
     1261 tgtgataaac cccatgatga ttgaggtcct ggaaaacttc gcgccattca agctgcgttt
     1321 tatcggtgat atctgccagc tcctgccata agctttcttt aatcacccgc ataaagacgc
     1381 tgtcagattc ttctttcagg ctctcttcat agctcatggc gaactcttcc aggtcacgtc
     1441 ttacagctcg cgtcagctca tcgacctcgg ttcgcgttaa ctcccctgtt tttattacgc
     1501 gctcgcgcgc ctgttccacc agtgcgtcga tatcacgttc gccattgcgc aggcgttcgc
     1561 tcagtgacgc gaccagttca cggtaatatt gagcaacctt gttcatcgtt tcatctcccg
     1621 ggtcgttaac tgactataat aatagacgtt ctttgttcct tgctttgcta atacgcccac
     1681 aaactctgta aattaatgta tgaataatcc gggcatcatt tcaacgtcga gtgcgcgaaa
     1741 ggctgttttg acgcggacgt ttgggctatg ctatgcggat ctgaaaaacc acctcaacgc
     1801 tacatttgta gccgtattga aaacaggacc actggctgcc atgcaagagc aataccgccc
     1861 ggaagagata gaatccaaag tacagcttca ttgggatgag aagcgcacat ttgaagtaac
     1921 cgaagacgag agcaaagaga agtattactg cctgtctatg cttccctatc cttctggtcg
     1981 actacacatg ggccacgtac gtaactacac catcggtgac gtgatcgccc gctaccagcg
     2041 tatgctgggc aaaaacgtcc tgcagccgat cggctgggac gcgtttggtc tgcctgcgga
     2101 aggcgcggcg gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca acatcgcgta
     2161 tatgaaaaac cagctcaaaa tgctgggctt tggttatgac tggagccgcg agctggcaac
     2221 ctgtacgccg gaatactacc gttgggaaca gaaattcttc accgagctgt ataaaaaagg
     2281 cctggtatat aagaagactt ctgcggtcaa ctggtgcccg aacgaccaga ccgtactggc
     2341 gaacgaacaa gttatcgacg gctgctgctg gcgctgcgat accaaagttg aacgtaaaga
     2401 gatcccgcag tggtttatca aaatcactgc ttacgctgac gagctgctca acgatctgga
     2461 taaactggat cactggccag acaccgttaa aaccatgcag cgtaactgga tcggtcgttc
     2521 cgaaggcgtg gagatcacct tcaacgttaa cgactatgac aacacgctga ccgtttacac
     2581 tacccgcccg gacaccttta tgggttgtac ctacctggcg gtagctgcgg gtcatccgct
     2641 ggcgcagaaa gcggcggaaa ataatcctga actggcggcc tttattgacg aatgccgtaa
     2701 caccaaagtt gccgaagctg aaatggcgac gatggagaaa aaaggcgtcg atactggctt
     2761 taaagctgtt cacccattaa cgggcgaaga aattcccgtt tgggcagcaa acttcgtatt
     2821 gatggagtac ggcacgggcg cagttatggc ggttccgggt cacgaccagc gcgactacga
     2881 gtttgcctct aaatacggcc tgaacatcaa accagttatc ctggctgctg acggctctga
     2941 gccagatctt tctcagcaag ccctgactga aaaaggcgtg ctgttcaact ctggcgaatt
     3001 caacggtctt gaccatgaag cggccttcaa cgccatcgcc gataaactga ctgcgatggg
     3061 cgttggcgag cgtaaagtga actaccgcct gcgcgactgg ggtgtttctc gtcagcgtta
     3121 ctggggcgcg ccgattccga tggtgacgct ggaagacggt accgtaatgc cgaccccgga
     3181 cgaccagctg ccggtgatcc tgccggaaga tgtggtgatg gacggcatta ccagcccgat
     3241 taaagcagat ccggagtggg caaaaactac cgttaacggt atgccagcgc tgcgtgaaac
     3301 cgacactttc gacaccttta tggagtcctc ctggtactat gcgcgctaca cttgcccaga
     3361 gtacaaagaa ggtatgctgg attccgaagc ggctaactac tggctgccgg tggatatcta
     3421 cattggtggt atcgaacacg ccattatgca cctgctctac ttccgcttct tccacaaact
     3481 gatgcgtgat gcaggcatgg tgaactctga cgaaccagcg aaacagttgc tgtgtcaggg
     3541 tatggtgctg gcagatgcct tctactatgt tggcgaaaac ggcgaacgta actgggtttc
     3601 cccggttgat gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag cgaaagatgc
     3661 ggcaggccat gaactggttt ataccggcat gagcaaaatg tccaagtcga agaacaacgg
     3721 tatcgacccg caggtgatgg ttgaacgtta cggcgcggac accgttcgtc tgtttatgat
     3781 gtttgcttct ccggctgata tgactctcga atggcaggaa tccggtgtgg aaggggctaa
     3841 ccgcttcctg aaacgtgtct ggaaactggt ttacgagcac acagcaaaag gtgatgttgc
     3901 ggcactgaac gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg atgtgcataa
     3961 aacgatcgct aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca ccgcaattgc
     4021 ggcgattatg gagctgatga acaaactggc gaaagcacca accgatggcg agcaggatcg
     4081 cgctctgatg caggaagcgc tgctggccgt tgtccgtatg cttaacccgt tcaccccgca
     4141 catctgcttc acgctgtggc aggaactgaa aggcgaaggc gatatcgaca acgcgccgtg
     4201 gccggttgct gacgaaaaag cgatggtgga agactccacg ctggtcgtgg tgcaggttaa
     4261 cggtaaagtc cgtgccaaaa tcaccgttcc ggtggacgca acggaagaac aggttcgcga
     4321 acgtgctggc caggaacatc tggtagcaaa atatcttgat ggcgttaccg tgcgtaaagt
     4381 gatttacgta ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg aggaagcgtg
     4441 cgatatctgg caacattgtt gttatctctg gctgtgttaa tcaccgccgg gtgtggctgg
     4501 catctgcgtg ataccacgca ggttccttcc actatgaagg tcatgatcct ggactcaggc
     4561 gatccgaacg ggccattaag ccgtgcggtg cgtaaccagt tacgtctgaa tggtgtcgag
     4621 ttgcttgata aagaaaccac gcgtaaggac gttccatccc tgcgtttggg taaagtgagc
     4681 atcgcgaaag ataccgcatc ggtattccgt aacggtcaaa cagcagagta tcagatgatc
     4741 atgacggtga atgcgaccgt gttgatcccc ggccgtgata tctacccgat tagtgccaaa
     4801 gtcttccgtt cgttctttga taacccgcaa atggcgttag cgaaagataa cgaacaagac
     4861 atgatcgtaa aagagatgta cgaccgtgct gccgaacagc tgattcgtaa gctgccaagc
     4921 atccgtgctg cggatattcg ttccgacgaa gaacagacgt cgaccacaac ggatactccg
     4981 gcaacgcctg cacgcgtctc caccacgctg ggtaactgat gattcggttg tacccggaac
     5041 aactccgcgc gcagctcaat gaagggctgc gcgcggcgta tcttttactt ggtaacgatc
     5101 ctctgttatt gcaggaaagc caggacgctg ttcgtcaggt agctgcggca caaggattcg
     5161 aagaacacca cactttttcc attgatccca acactgactg gaatgcgatc ttttcgttat
     5221 gccaggctat gagtctgttt gccagtcgac aaacgctatt gctgttgtta ccagaaaacg
     5281 gaccgaatgc ggcgatcaat gaacaacttc tcacactcac cggacttctg catgacgacc
     5341 tgctgttgat cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc gcctggttta
     5401 ctgctcttgc gaatcgcagc gtgcaggtga cctgtcagac accggagcag gctcagcttc
     5461 cccgctgggt tgctgcgcgc gcaaaacagc tcaacttaga actggatgac gctgcaaatc
     5521 aggtgctctg ctactgttat gaaggtaacc tgctggcgct ggctcaggca ctggagcgtt
     5581 tatcgctgct ctggccagac ggcaaattga cattaccgcg cgttgaacag gcggtgaatg
     5641 atgccgcgca tttcacccct tttcattggg ttgatgcttt gttgatggga aaaagtaagc
     5701 gcgcgttgca tattcttcag caactgcgtc tggaaggcag cgaaccggtt attttgttgc
     5761 gcacattaca acgtgaactg ttgttactgg ttaacctgaa acgccagtct gcccatacgc
     5821 cactgcgtgc gttgtttgat aagcatcggg tatggcagaa ccgccggggc atgatgggcg
     5881 aggcgttaaa tcgcttaagt cagacgcagt tacgtcaggc cgtgcaactc ctgacacgaa
     5941 cggaactcac cctcaaacaa gattacggtc agtcagtgtg ggcagagctg gaagggttat
     6001 ctcttctgtt gtgccataaa cccctggcgg acgtatttat cgacggttga tatgaaatct
     6061 ttacaggctc tgtttggcgg cacctttgat ccggtgcact atggtcatct taaacccgtg
     6121 gaaacgctgg cgaatttgat tggtctgacg cgggtcacaa tcatccctaa taatgttcct
     6181 ccgcatcgtc cccagccgga agcgaacagc gtgcagcgta aacacatgct tgaactggcg
     6241 attgccgaca agccattatt tactcttgat gaacgcgagc taaagcgcaa tgccccctct
     6301 tacactgcgc aaacactgaa agagtggcgg caggaacaag ggccggacgt gccgctggcg
     6361 tttattattg gtcaggattc attgctgacc tttccgacct ggtacgaata cgaaacgata
     6421 ctcgacaatg cacatttgat cgtctgtcgg cgtccaggtt acccacttga aatggcgcaa
     6481 ccgcaatacc agcaatggct ggaagatcat ttgacacata acccggaaga tcttcacctt
     6541 cagcctgccg gtaaaattta tctggctgaa acgccgtggt ttaacatctc ggcgaccatc
     6601 atccgcgaac gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga accggtactg
     6661 acttacatta accaacaagg cttgtatcgc tgatacctgt cgttacggag ggcatgatgc
     6721 gactgtggtt aattcgtcat ggtgaaacgc aagcgaatat cgatggtctt tacagcggtc
     6781 atgcgcccac ccccctgacc gcgcgcggta ttgagcaagc gcaaaatctg catacactgc
     6841 tagatgatgt ttcctttgac ctggttttat gcagtgaact ggaacgggca cagcataccg
     6901 cgcgacgggt tctcagtgac cgccagctcc ccgtgcaaat catccctgaa ctcaacgaaa
     6961 tgttttttgg cgactgggag atgcgacatc atcgcgacct catgcaagaa gatgccgaaa
     7021 actatagcgc gtggtgcaat gactggcagc atgctatccc cacgaacggt gaaggatttc
     7081 aggcattttc gcaacgtgtg gaaagattta tcgcaaggct tagtgaatat cagcactatc
     7141 agaatatttt aatcgtcagc catcagggcg tactgagtct gttaattgct cgtctaattg
     7201 gcatgcctgc cgaatctatg tggcattttc gcgttgacca ggggtgttgg agcgccattg
     7261 atatcaacca aaaattcgcg acgctacgcg tcctcaatag ccgtgccatc ggggtcgaga
     7321 atgcatgact tttctgtttt tttacgggta agccgcaacg accattgaca gccccgggca
     7381 ggctgatatt ctccgcagcc agactttttc cgccagacac gactttgtag aaattgtttt
     7441 acaaaaatgg cgatgcaatc tgcggcgcgg ggtgggatga tagcccactt tcgaaagccg
     7501 attcggcgac aattgtcccg aaatcgcctc tggttcaggt atactgacag accattttta
     7561 tctatttgat tcacccaggg ggaaaacttg cagggtaaag cactccagga ttttgttatc
     7621 gacaaaattg atgacctcaa aggtcaggac atcatcgcct tagacgttca gggcaaatcc
     7681 agcatcaccg actgcatgat catctgtacg ggtacgtcca gccgtcatgt tatgtccatt
     7741 gctgaccacg ttgtgcagga gtctcgcgca gcgggcctgt taccgctcgg cgtagaaggt
     7801 gaaaacagcg ccgactggat tgtcgtggat ttgggcgatg tgattgtcca tgtcatgcag
     7861 gaagagagcc gtcgcctgta tgaactggaa aaactctgga gttaatgcgt gaagctgcaa
     7921 cttgtcgccg tgggaacgaa aatgccggac tgggtacaaa ccggttttac cgagtacctg
     7981 cgtcgttttc cgaaagatat gcccttcgag ctgattgaaa ttccggccgg aaaacgcggc
     8041 aagaatgcgg acatcaagcg catactcgac aaagagggtg agcagatgtt ggcggccgca
     8101 ggcaaaaacc gcattgtcac cctcgatatt ccaggcaagc cctgggatac gccgcagtta
     8161 gccgctgagc tggaacgctg gaagctggat ggtcgcgacg tcagtctact gattggcggg
     8221 cctgaagggt tgtcgcctgc ctgtaaagcg gcggcggagc agagctggtc gctgtcggcg
     8281 cttaccctcc cccatccgct ggttcgcgtg ctggtcgcag agagtctgta ccgggcgtgg
     8341 agcatcacca ccaaccatcc ttatcaccgt gagtgataaa ggagctttga gtagaaaacg
     8401 cagcggatga aactacagaa ctcttttcgc gactatacgg cagagtccgc gctgtttgtg
     8461 cgccgggcgc tggtcgcctt tttggggatt ttgctgctga ccggcgtgct tatcgccaac
     8521 ctgtataatc tgcaaattgt tcgctttacc gattaccaga cccgctctaa tgaaaaccgc
     8581 attaagctgg tgcctatcgc gcccagccgc ggcattatct acgaccgtaa cggtatccct
     8641 ctggcactca accgcactat ctaccagata gaaatgatgc cggagaaagt cgataacgtg
     8701 cagcaaacgc tggacgcttt gcgcagcgtg gttgatctga ccgatgacga tattgctgca
     8761 ttccgaaaag agcgcgcacg ttcacaccgt ttcacctcta ttcccgtgaa aactaacctg
     8821 accgaagtac aagtagctcg ctttgccgtc aatcagtacc gttttccggg tgtcgaagtt
     8881 aaaggctata aacgtcgtta ctatccttac ggttcggcgt tgacccacgt catcggctat
     8941 gtgtcgaaaa tcaacgataa agacgtcgaa cgcctgaata atgacggcaa actggccaac
     9001 tatgcggcaa cgcatgatat cggtaagctg ggcattgagc gttactatga agacgtgctg
     9061 cacggtcaga ccggttatga agaggttgaa gttaacaacc gtgggcgtgt tatccgccag
     9121 ttaaaagaag taccaccgca agccggacac gatatttacc tgacgctgga tctcaaactc
     9181 cagcaatata ttgaaacgct gctggcgggt agccgcgcag ctgtggtagt caccgatccg
     9241 cgtacaggtg gggtgctggc actggtttcc acgcctagtt atgacccaaa cctgtttgtt
     9301 gacggtatct ccagcaaaga ttattccgcc ttgctgaacg acccgaatac accgctggtg
     9361 aaccgcgcca cacagggggt ttatcctcct gcgtctacag ttaaacccta tgtggcggtt
     9421 tcggcattaa gcgccggggt gatcacgcgc aatacgacgc tgtttgaccc aggctggtgg
     9481 caactaccag ggtcggaaaa acgttatcgt gactggaaaa aatggggcca cgggcgtctg
     9541 aatgtcacaa gatcgctgga agaatctgcg gataccttct tctatcaggt ggcctacgat
     9601 atggggatcg atcgcctctc cgaatggatg ggtaaattcg gttatggtca ttacaccggt
     9661 atcgacctgg cggaagaacg ttccggcaac atgcctaccc gcgaatggaa acagaaacgc
     9721 tttaaaaaac cgtggtatca gggtgacacc attccggttg gtatcggcca gggttactgg
     9781 acagcgaccc caatccagat gagtaaggca ctgatgatcc tgattaatga cggtatcgtg
     9841 aaggttccgc atttgctgat gagcaccgcc gaagacggca aacaggtgcc atgggtacaa
     9901 ccgcatgaac cgcccgtcgg cgatattcat tccggttact gggagctggc gaaagacggt
     9961 atgtacggtg ttgctaaccg ccctaacggt acggcgcata aatactttgc cagcgcaccg
    10021 tacaaaattg cggcgaaatc tggtaccgct caggtcttcg gtctgaaagc gaacgaaacc
    10081 tataatgcgc acaaaattgc cgagcgttta cgtgaccaca aactgatgac cgcctttgcg
    10141 ccatacaaca atccgcaagt ggctgtcgcc atgattctgg agaacggcgg cgcgggtccg
    10201 gcggttggta cgctgatgcg ccagatcctc gaccacatta tgctgggtga taacaatacc
    10261 gatctgcctg cggaaaatcc tgcggttgcc gcagcggagg accattaatc atgacggata
    10321 atccgaataa aaaaacattc tgggataaag tccatctcga tcccacaatg ctgctgatct
    10381 tactggcatt gctggtttac agcgccctgg ttatctggag cgccagcggt caggatattg
    10441 gcatgatgga gcgtaaaata ggccaaatcg cgatgggtct ggtcatcatg gtggtgatgg
    10501 cgcaaattcc tccacgcgtt tatgaaggct gggcccccta tctctatatc atctgtatta
    10561 ttttgctggt ggcggtagat gctttcggtg ccatctctaa aggcgctcaa cgctggctgg
    10621 acctcggtat tgttcgtttt cagccgtcgg aaattgccaa aatagccgta ccactgatgg
    10681 ttgcgcgctt tatcaaccgc gacgtttgcc cgccatcgtt gaagaacact ggcatcgcgc
    10741 tggtgctgat atttatgccc acgctgctgg tggctgcaca gcctgacctg ggaacatcaa
    10801 tcctcgttgc gctttccggt ctgtttgtac tgttcctctc tggccttagc tggcgtctga
    10861 ttggcgtcgc agtagtgctg gtggcggcgt tcattcctat tctgtggttc ttcctgatgc
    10921 atgattacca gcgccagcgc gtaatgatgc tcctggaccc ggaatcagac ccactcggcg
    10981 cgggctatca cattattcag tctaaaattg ctattggctc cggcggatta cgcggcaaag
    11041 gctggctgca tggcactcag tcacaacttg aatttctacc cgaacgccat accgacttta
    11101 tcttcgcggt actggcggaa gagctgggat tagtgggcat tctgattctg ctcgctctct
    11161 acattctgct gatcatgcgc gggctgtgga tagccgccag agcgcaaacc acctttggtc
    11221 gcgtcatggc tggcggctta atgctgatat tattcgttta tgtcttcgta aatattggta
    11281 tggtaagcgg tattctgccg gttgtagggg ttccgctccc actggtcagt tatggaggat
    11341 cagcgctaat tgtgctgatg gctgggttcg ggattgtaat gtcaatccac acccacagga
    11401 aaatgttgtc gaaaagcgtg taagaggtgc gcaatgcgta agcagtggct cgggatctgc
    11461 atcgcggcag gaatgctcgc ggcatgtaca agcgatgatg gtcagcaaca gacggtaagt
    11521 gtaccgcagc ctgcggtatg taacggcccc atagttgaaa ttagcggggc ggacccgcgt
    11581 ttcgaaccac tgaacgcgac ggcaaatcag gattaccagc gcgacggtaa aagctacaaa
    11641 atcgtgcagg atccgtctcg atttagccag gcgggactgg cggcaatcta tgatgccgaa
    11701 ccaggcagta acctgacggc ctctggcgaa gctttcgatc cgacacagtt gacggcggcc
    11761 catccaacgc ttccgatccc cagctacgcc agaatcacta acctggctaa cgggcgaatg
    11821 atcgtggtgc gcattaatga tcgcggcccc tacggcaacg accgcgtaat ttcgctttct
    11881 cgcgcagcag ctgaccgtct taacacgtca aacaacacca aagttcgtat cgatccgatt
    11941 attgtcgccc aggatggttc gctttctggt cctggtatgg cttgtaccac agtcgccaaa
    12001 cagacttacg ccctgcctgc acctcccgat ttaagcggtg gcgcgggggc aagttcagtg
    12061 tctggcccgc agggtgacat tcttccggtc agtaactcga cgctaaaaag cgaagatccg
    12121 accggcgcgc cggtaaccag tagcggtttc ctcggcgctc caacgacctt agcgcctggt
    12181 gtactggaaa gcagcgaacc gacacctgca ccacagcccg ttgttacagc tccgtcgaca
    12241 acgcctgcaa cctcgcctgc aatggtgaca ccgcaagccg cctcgcaaag cgccagcggc
    12301 aactttatgg tgcaagtcgg ggccgttagc gatcaggctc gtgcgcaaca gtaccaacag
    12361 caactgggac agaagttcgg cgtccccggt cgcgtaactc aaaatggcgc ggtctggcgt
    12421 atccagcttg gcccattcgc cagtaaagcc gaagccagta ccttgcagca acgtttgcaa
    12481 accgaagccc aattacagtc atttattacc accgcgcagt agcgtaaagc aggcatctga
    12541 tgtgtcaatt tctgtaaatg acgttaacaa agtcacgcgg aaagtcagat gcctgccggt
    12601 agtgcatttg ctatagtagg gcactttttt taattccatc acggatgtcg tagttcagac
    12661 catgaatacc attttttccg ctcgtatcat gaagcgcctg gcgctcacca cggctctttg
    12721 cacagccttt atctctgctg cacatgccga tgacctgaat atcaaaacta tgatcccggg
    12781 tgtaccgcag atcgatgcgg agtcctacat cctgattgac tataactccg gcaaagtgct
    12841 cgccgaacag aacgcagatg tccgccgcga tcctgctagc ctgaccaaaa tgatgaccag
    12901 ttacgttatc ggccaggcaa tgaaagccgg taaatttaaa gaaactgatt tagtcactat
    12961 cggcaacgac gcatgggcca ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct
    13021 caaaccgggc atgcaggttc cggtttctca gctgatccgc ggtattaacc tgcaatcggg
    13081 taacgatgct tgcgtcgcca tggccgattt tgccgctggt agccaggacg cttttgttgg
    13141 cttgatgaac agctacgtta acgccctggg cctgaaaaac acccacttcc agacggtaca
    13201 tggtctggat gctgatggtc agtacagctc cgcgcgagat atggcgctga tcggccaggc
    13261 attgatccgt gacgtaccga atgaatactc gatctataaa gaaaaagaat ttacgtttaa
    13321 cggtattcgc cagctgaacc gtaacggcct gttatgggat aacagcctga atgtcgacgg
    13381 catcaaaacc ggacacactg acaaagcagg ttacaacctt gttgcttctg cgactgaagg
    13441 ccagatgcgc ttgatttctg cggtgatggg cggacgtact tttaaaggcc gtgaagccga
    13501 aagtaaaaaa ctgctaacct ggggcttccg tttcttcgaa accgttaacc cactgaaagt
    13561 aggtaaagag ttcgcctctg aaccggtttg gtttggtgat tctgatcgcg cttcgttagg
    13621 ggttgataaa gacgtgtacc tgaccattcc acgtggtcgc atgaaagatc tgaaagccag
    13681 ctatgtgctg aacagcagtg aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac
    13741 tatcaacttc cagcttgatg gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga
    13801 aatcccggaa ggtaacttct tcggcaaaat cattgattac attaaattaa tgttccatca
    13861 ctggtttggt taaaaattga acacttgaaa gtgtaatttc cgtccccata tactaagcat
    13921 cagtaaaaaa actcccgcct tctggcggga gttgctattt aattacgtta cgccggagct
    13981 gacatgaaaa ccaaacttaa cgaactgctt gaattcccta ctccttttac ttacaaagtt
    14041 atggggcagg cgttacctga gctggttgat caggtggttg aagtggtaca gcgccatgcg
    14101 ccaggtgact acaccccaac ggtaaaacca agcagcaaag gcaactacca ctcggtatct
    14161 atcactatca acgccactca tatcgagcag gttgaaacac tgtatgaaga actgggcaaa
    14221 atcgatattg tccgcatggt tctgtaactc gcttctccgt tacccggctc ctggtcgggt
    14281 aactcccctc tcctcgctgt gatatacttt tcccccactt ttactcattc tccacggaga
    14341 tgccgttttg tatcaggata aaattcttgt ccgccagctc ggtcttcagc cttacgagcc
    14401 aatctcccag gctatgcatg aattcaccga tacccgcgat gatagtaccc ttgatgaaat
    14461 ctggctggtc gagcactatc cggtattcac ccaaggtcag gcaggaaaag cggagcacat
    14521 tttaatgccg ggtgatattc cggtgatcca gagcgatcgc ggtgggcagg tgacttatca
    14581 cgggccggga caacaggtga tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt
    14641 gcgtgaactg gtgaccttgc ttgagcaaac agtggtgaat accctggctg aactgggtat
    14701 agaagcgcat cctcgggctg acgcgcctgg tgtctatgtc ggggaaaaga aaatttgctc
    14761 actgggttta cgaattcgac gcggttgttc attccacggt ctggcattaa acgtcaatat
    14821 ggatctttca ccatttttac gtattaatcc ttgtgggtat gccggaatgg aaatggctaa
    14881 aatatcacaa tggaaacccg aagcgacgac taataatatt gctccacgtt tactggaaaa
    14941 tattttagcg ctactaaaca atccggactt cgaatatatt accgcttaat tccatacatc
    15001 aatggcccaa tttacactgg gtcattactc ataatacctg ctaatgcttt acatctcctt
    15061 cgcgtttgta tattttcttt atcacttgca gaaatgcggt gccacgcctt gctccgcaac
    15121 tcaccccatt gattaaccta ttgaaatagt tgattatggt gagcaaaagc gttccgttcc
    15181 aattagacat aagattattg taatttttta aatattat
//
LOCUS       DAZFWL020000113        15199 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFWL020000113 DAZFWL020000000
VERSION     DAZFWL020000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.113"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            202..537
                     /locus_tag="ACHKNP_RS18395"
                     /old_locus_tag="pgaptmp_003679"
     CDS             202..537
                     /locus_tag="ACHKNP_RS18395"
                     /old_locus_tag="pgaptmp_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGU4705772.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            697..1140
                     /gene="yjdN"
                     /locus_tag="ACHKNP_RS18400"
                     /old_locus_tag="pgaptmp_003680"
     CDS             697..1140
                     /gene="yjdN"
                     /locus_tag="ACHKNP_RS18400"
                     /old_locus_tag="pgaptmp_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HGU4705773.1"
                     /translation="MSLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1273..2061
                     /gene="phnC"
                     /locus_tag="ACHKNP_RS18405"
                     /old_locus_tag="pgaptmp_003681"
     CDS             1273..2061
                     /gene="phnC"
                     /locus_tag="ACHKNP_RS18405"
                     /old_locus_tag="pgaptmp_003681"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HGU4705774.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2086..3102
                     /gene="phnD"
                     /locus_tag="ACHKNP_RS18410"
                     /old_locus_tag="pgaptmp_003682"
     CDS             2086..3102
                     /gene="phnD"
                     /locus_tag="ACHKNP_RS18410"
                     /old_locus_tag="pgaptmp_003682"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4705775.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3208..3987
                     /gene="phnE"
                     /locus_tag="ACHKNP_RS18415"
                     /old_locus_tag="pgaptmp_003683"
     CDS             3208..3987
                     /gene="phnE"
                     /locus_tag="ACHKNP_RS18415"
                     /old_locus_tag="pgaptmp_003683"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HGU4705776.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRVINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4008..4733
                     /gene="phnF"
                     /locus_tag="ACHKNP_RS18420"
                     /old_locus_tag="pgaptmp_003684"
     CDS             4008..4733
                     /gene="phnF"
                     /locus_tag="ACHKNP_RS18420"
                     /old_locus_tag="pgaptmp_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HGU4705777.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4734..5186
                     /gene="phnG"
                     /locus_tag="ACHKNP_RS18425"
                     /old_locus_tag="pgaptmp_003685"
     CDS             4734..5186
                     /gene="phnG"
                     /locus_tag="ACHKNP_RS18425"
                     /old_locus_tag="pgaptmp_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HGU4705778.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5183..5767
                     /gene="phnH"
                     /locus_tag="ACHKNP_RS18430"
                     /old_locus_tag="pgaptmp_003686"
     CDS             5183..5767
                     /gene="phnH"
                     /locus_tag="ACHKNP_RS18430"
                     /old_locus_tag="pgaptmp_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HGU4705779.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5767..6831
                     /gene="phnI"
                     /locus_tag="ACHKNP_RS18435"
                     /old_locus_tag="pgaptmp_003687"
     CDS             5767..6831
                     /gene="phnI"
                     /locus_tag="ACHKNP_RS18435"
                     /old_locus_tag="pgaptmp_003687"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HGU4705780.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6824..7669
                     /gene="phnJ"
                     /locus_tag="ACHKNP_RS18440"
                     /old_locus_tag="pgaptmp_003688"
     CDS             6824..7669
                     /gene="phnJ"
                     /locus_tag="ACHKNP_RS18440"
                     /old_locus_tag="pgaptmp_003688"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HGU4705781.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7666..8424
                     /gene="phnK"
                     /locus_tag="ACHKNP_RS18445"
                     /old_locus_tag="pgaptmp_003689"
     CDS             7666..8424
                     /gene="phnK"
                     /locus_tag="ACHKNP_RS18445"
                     /old_locus_tag="pgaptmp_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HGU4705782.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMGEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8535..9215
                     /gene="phnL"
                     /locus_tag="ACHKNP_RS18450"
                     /old_locus_tag="pgaptmp_003690"
     CDS             8535..9215
                     /gene="phnL"
                     /locus_tag="ACHKNP_RS18450"
                     /old_locus_tag="pgaptmp_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HGU4705783.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            9212..10348
                     /gene="phnM"
                     /locus_tag="ACHKNP_RS18455"
                     /old_locus_tag="pgaptmp_003691"
     CDS             9212..10348
                     /gene="phnM"
                     /locus_tag="ACHKNP_RS18455"
                     /old_locus_tag="pgaptmp_003691"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HGU4705784.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CHARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10348..10905
                     /gene="phnN"
                     /locus_tag="ACHKNP_RS18460"
                     /old_locus_tag="pgaptmp_003692"
     CDS             10348..10905
                     /gene="phnN"
                     /locus_tag="ACHKNP_RS18460"
                     /old_locus_tag="pgaptmp_003692"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HGU4705785.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10892..11326
                     /gene="phnO"
                     /locus_tag="ACHKNP_RS18465"
                     /old_locus_tag="pgaptmp_003693"
     CDS             10892..11326
                     /gene="phnO"
                     /locus_tag="ACHKNP_RS18465"
                     /old_locus_tag="pgaptmp_003693"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HGU4705786.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11328..12086
                     /gene="phnP"
                     /locus_tag="ACHKNP_RS18470"
                     /old_locus_tag="pgaptmp_003694"
     CDS             11328..12086
                     /gene="phnP"
                     /locus_tag="ACHKNP_RS18470"
                     /old_locus_tag="pgaptmp_003694"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HGU4705787.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12233..12562)
                     /gene="yjdP"
                     /locus_tag="ACHKNP_RS18475"
                     /old_locus_tag="pgaptmp_003695"
     CDS             complement(12233..12562)
                     /gene="yjdP"
                     /locus_tag="ACHKNP_RS18475"
                     /old_locus_tag="pgaptmp_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HGU4705788.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12631..12948)
                     /locus_tag="ACHKNP_RS18480"
                     /old_locus_tag="pgaptmp_003696"
     CDS             complement(12631..12948)
                     /locus_tag="ACHKNP_RS18480"
                     /old_locus_tag="pgaptmp_003696"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HGU4705789.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13201..15186
                     /gene="yjcS"
                     /locus_tag="ACHKNP_RS18485"
                     /old_locus_tag="pgaptmp_003697"
     CDS             13201..15186
                     /gene="yjcS"
                     /locus_tag="ACHKNP_RS18485"
                     /old_locus_tag="pgaptmp_003697"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HGU4705790.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         3441 a         4499 c         4183 g         3076 t
ORIGIN      
        1 agagcattaa cattatctta aataataaat ggaggcaaaa agattatttt ctgtttgcgt
       61 ttcctttcaa atgaaaacga tcgtcgtcta aaatcagcag tacccccgac aaactcaggg
      121 attttgtgta taattgcggc ctttttcggc aatctgccgt tttttggcgc ttttgccctg
      181 ctgacttttg aggaaatcca catgtcatta ccacactgcc caaaatgcaa ctccgaatac
      241 acttacgaag ataacggcat gtacatctgc ccggaatgtg cctacgaatg gaacgacgca
      301 gaacctgcac aggaaagcga cgagctgatc gttaaagatg ctaacggcaa tctgctggct
      361 gacggcgaca gcgttaccat cattaaagat ctgaaggtga aaggtagctc ttcgatgctg
      421 aaaattggca ccaaagtgaa aaacatccgc ctggttgaag gcgaccataa catcgattgc
      481 aaaatcgacg gttttggtcc gatgaaactg aaatctgagt ttgtgaaaaa gaactgattg
      541 tattgtgatc ggtaagccgg ataaggcgct cgcgccgcat ccggcaacga tgccgactgc
      601 ctgatgcgac gctgaccgcg tcttatcagg cttccctctc atctgtataa atttcgaact
      661 acacttaact ggcttctctt aactgaggtc accatcatgt cgttaagtcc ctacctctct
      721 tttgccggta actgtgccga cgcaattgct tattatcaac gtacgttggg cgctgaactg
      781 ctctataaaa tcagcttcgg cgaaatgcca aaatcagcgc aggacagcgc cgagaactgc
      841 ccttccggaa tgcaatttcc cgataccgcc atcgcccacg ccaacgtgcg cattgccgga
      901 agcgacatca tgatgagcga tgctataccg tcaggaaaag ccagctactc cggctttacg
      961 ctggtgctcg attcgcaaca ggtcgaagaa ggaaaacgct ggtttgacaa tcttgccgct
     1021 aacggaaaaa tcgaaatggc ctggcaggaa actttctggg cgcatggctt tggcaaagtc
     1081 accgataaat ttggcgtacc gtggatgatt aatgtcgtca aacaacaacc aacgcaataa
     1141 cccgccggga ggcccgccct cccgcactgt catcgaattc ccgttaactc ttcatctgtt
     1201 agtcactttt aattaaccaa atcgtcacaa taatccgcca cgatggagcc acttttttag
     1261 ggaggctgca tcatgcaaac gattatccgt gtcgagaagc tcgccaaaac cttcaatcag
     1321 catcaggcgc tgcatgcggt tgatctgaac attcatcacg gtgaaatggt ggctctgctt
     1381 gggccgtcgg gttccggcaa atccaccctt ttacgtcact taagcggttt gattaccggc
     1441 gataaatccg ccggcagcca tatcgagctg ctgggccgca cagtccagcg cgaaggccgt
     1501 ctggcgcgcg atatccgcaa aagccgcgcc aacaccggct acatcttcca acaattcaac
     1561 ctggtgaacc gcctgagcgt actggagaac gtgctgattg gcgcgctcgg cagcacgccg
     1621 ttctggcgca cctgttttag ctggttcacc ggcgagcaga aacagcgcgc gttacaggcg
     1681 ctgacccgcg ttggcatggt gcattttgcc catcagcgcg tttccaccct ctccggcgga
     1741 cagcagcagc gtgtggcgat tgcccgcgcg ctgatgcagc aggcgaaggt gattctggcc
     1801 gatgaaccca tcgcctcgct ggacccggaa tccgcccgca tcgtgatgga caccctgcgc
     1861 gacatcaatc agaacgacgg catcaccgtg gtcgtcacgc tgcatcaggt ggattacgcc
     1921 ctgcgctact gcgaacgcat cgtcgccctg cgccaggggc acgttttcta cgacggcagc
     1981 agccaacagt ttgataacga acgttttgac catctctacc gcagcattaa tcgcatcgaa
     2041 gagaacgcga aagctgcctg acatccccat cattgaggaa aacgaatgaa cgctaagata
     2101 attgcctcgc tggccttcac cagcatgttc agcctcagca ccctgttaag cccggcacac
     2161 gccgaagagc aggaaaaggc gctgaatttc ggcattattt caacggaatc acagcaaaac
     2221 ctgaaaccgc aatggacgcc attcttacag gatatggaga agaagctggg cgtgaaggtg
     2281 aacgccttct ttgccccaga ctacgcaggc attatccagg gaatgcgctt caataaagtg
     2341 gatatcgcct ggtacggcaa cctgtcggca atggaagcgg tggatcgcgc caacggccag
     2401 gtcttcgccc agacggtcgc ggcggatgga tcgccaggtt actggagcgt gttgatcgtc
     2461 aacaaagata gtccgatcaa caacctgaac gatctgctgg cgaagcggaa agatctcacc
     2521 ttcggcaatg gcgatcctaa ctccacctct ggcttcctcg tccccggtta ctacgtcttt
     2581 gccaaaaaca atatctccgc cagcgacttt aagcgcaccg tcaacgctgg gcatgaaacc
     2641 aacgcgctgg ccgtcgccaa caagcaggtg gatgttgcca ccaacaacac cgaaaacctc
     2701 gacaagctga aaacctccgc gccagagaag ctgaaagaac tgaaggtgat ctggaagtcg
     2761 ccgctgatcc caggcgatcc gatcgtctgg cgtaaaaacc tttccgaaac caccaaagac
     2821 aagatctacg acttctttat gaactacggc aaaacgccgg aagaaaaagc ggtgctggaa
     2881 cgcctgggct gggcgccgtt ccgcgcttcc agcgacctgc aactggtgcc gattcgccag
     2941 ctcgcgctgt ttaaagagat gcagggcgtg aaaagcaata aaggactgaa tgagcaggac
     3001 aagctggcaa aaaccaccga gattcaggcg cagctggatg acctggaccg cctgaacaac
     3061 gcgttaagcg cgatgagttc ggtgagtaaa gcggtgcagt aaatcgtagg tcggataaga
     3121 cgccccggcg tcgcatccga caatgtgcag gcgttgatgc cggatgcggt gcaagcacct
     3181 tatccggcct acagaccgga gccaaacatg caaaccatca ccatcgcccc acccaagcgc
     3241 agctggttct cgcttctgag ctgggccgtt gttctcgccg tgttggtcgt ctcgtggcag
     3301 ggcgcggaaa tggccccgct cacgctgatt aaagacggcg gcaacatggc gacgttcgcc
     3361 gccgacttct tcccgcccga tttcagccag tggcaggatt acctcaccga aatggccgtc
     3421 acgctgcaaa tcgccgtctg gggcacggcg ctggcggtgg ttctctccat ccccttcggc
     3481 ctgatgagcg ccgaaaacct ggtgccgtgg tgggtttacc agcccgttcg ccgcctgatg
     3541 gacgcctgcc gcgtcattaa cgaaatggtc ttcgccatgc tgttcgtggt cgccgtcggc
     3601 ctcggcccgt tcgctggcgt gctggcgctg tttatccaca ccaccggcgt gctctccaaa
     3661 ctgctttccg aagcggtgga agcgattgaa cccggcccgg tggaaggcat tcgcgccacc
     3721 ggcgccaaca agctcgaaga gatcctctac ggcgtgctgc cgcaggtgat gccgctgctg
     3781 atctcctact ccctctatcg cttcgaatcc aacgtccgct cggcgaccgt cgtcggcatg
     3841 gtcggtgcag gcgggatcgg cgtcaccctg tgggaagcga ttcgcggttt ccagttccaa
     3901 caaacctgcg ccctgatggt gcttatcatc gtcacggtca gcctgctgga tttcctctct
     3961 caacggttgc gtaagcactt tatctgataa gcgaggcatt gatatctatg cacttgtcta
     4021 cacatccgac cagctaccca acacgctatc aggagatagc cgcaaaactt gagcaggagc
     4081 ttcgtcaaca ctaccgctgc ggcgactatc ttcccgccga gcagcaactg gcggcgcgct
     4141 ttgaggtgaa ccgccacacc ctgcgccgcg ccatcgacca actggtggaa aaaggctggg
     4201 tacagcgccg tcagggcgtc ggcgtgctgg tgctgatgcg cccgttcgat tacccgctca
     4261 acgcccaggc gcgttttagc cagaacctgc tggaccaggg tagccatccc accagcgaaa
     4321 aactgctgtc ggtattgcgc cccgcgtccg gtcacgtcgc tgacgcgctg gggattaccg
     4381 agggcgagaa cgttatccat ttgcgcaccc tgcgtcgcgt caacggcgtc gcgctctgtt
     4441 taatcgacca ctactttgcg gacctcaccc tttggccgac gctgcaacgc ttcgacagcg
     4501 gttcgctgca cgattttctg cgcgagcaaa ccggaattgc gctgcgccgc agccagacgc
     4561 ggatcagcgc ccgtcgcgcc caggccaaag agtgccagcg tcttgaaatt ccgaatatgt
     4621 cgccgctgct gtgcgtgcgc acccttaacc accgtgacgg tgaaagcagc ccggcggagt
     4681 actccgtcag cctgacgcgc gccgacatga ttgaattcac tatggagcac tgaatgcacg
     4741 cagataccgc gacccgccag cactggatgt ccgtactggc gcacagccaa ccggctgaac
     4801 tggcagcccg cctgaaagca ctgaacatca ccgccgacta tgaggttatc cgcgccgccg
     4861 aaaccggcct ggtgcagatt caggcgcgga tgggcggcac cggcgaacgc ttttttgccg
     4921 gcgacgccac gctcacccgc gccgccgtac gcctgactga cggcacgctc ggctacagct
     4981 gggtgctggg gcgcgacaaa cagcacgccg aacgctgcgc gctgattgac gcgctgatgc
     5041 agcaatctcg ctattttcaa aacttatcag aaacccttat tgccccgctg gacgctgacc
     5101 gtatggcacg cattgccgca cgccaggccg aagtgaacgc cagccgggtc gacttcttta
     5161 cgatggttcg cggagacaac gcatgaccct ggaaaccgct tttatgcttc ccgtgcagga
     5221 tgcccagcac agttttcgtc gcctgttaaa ggccatgagc gagccgggcg tgattgtcgc
     5281 cctgcatcag ctcaagcgcg gctggcagcc gctgaatatc gccaccacca gcgtgctgct
     5341 gacgctggcc gataacgaca cgccggtgtg gcttgccgcg ccattgagta acgatatcgt
     5401 cagccagagc ctgcgttttc ataccaacgc gccgctggtc agccagccgg aacaggcgac
     5461 cttcgcggtg acggatgagg cgatttccag cgaacagctt aacgcccttt ccaccggcac
     5521 cgccgttgcg ccggaagcgg gtgcaacgct gattttacag gtcgccagcc tgagcggcgg
     5581 gcgcatgtta cgtctcaccg gcgcgggcat tgccgaagaa cgaatgatcg ctccgcagct
     5641 gccggagtgc attctgcacg aactcaccga gcgcccgcac ccgttcccgc tcggcatcga
     5701 cctgatcctg acctgcggcg agcgcctgct ggctattccg cgaaccactc atgtggaggt
     5761 gtgctgatgt acgttgccgt gaaagggggc gagaaggcga tcgacgccgc tcacgccctg
     5821 caagagagcc gacgccgggg cgataccgat ttgcctgaac tgagcgtcgc ccagattgaa
     5881 cagcagctta atcttgcggt ggatcgcgtg atgaccgaag gcggcattgc cgaccgcgaa
     5941 ctggcggcgc tggcgctgaa acaggccagc ggcgataacg tcgaagcaat tttcctgctg
     6001 cgcgcctacc gcaccacgct ggcgaagctc gcggtaagcg agccgctcga caccacagag
     6061 atgcgtctcg aacggcgtat ttcggcggtt tataaagaca ttcccggcgg ccagctgctt
     6121 ggcccaacct acgactacac ccatcgcctg ctcgatttca ccctgctggc aaacggtgaa
     6181 gcgccgacgc tgaccaccgc cgacagcgaa caacagccgt cgccgcacgt tttcagcctg
     6241 ctggcgcgtc aggggctggt gaagtttgaa gaagatagcg gcgcacagcc ggatgacatc
     6301 acccgcacgc cgccggttta cccctgctca cgctcctccc gcctgcaaca attaatgcgc
     6361 ggggacgaag gctatttgct ggcgctggcc tactccaccc agcgtggtta cgggcgcaat
     6421 cacccgttcg cgggcgagat ccgcagtggt tacatcgacg tgtcgattgt gccggaagag
     6481 ctgggatttg cggtaaacgt cggcgaacta ctgatgaccg agtgtgaaat ggtcaacggt
     6541 tttatcgacc cgccggatga gccgccgcac ttcacgcgcg gctacgggct ggtattcggc
     6601 atgagcgagc gcaaagcgat ggcaatggcg ctggtcgatc gtgcgttgca ggctccggaa
     6661 tacggcgagc acgcgacagg cccggcgcag gatgaagagt ttgtgctggc acatgccgac
     6721 aacgtcgaag ccgcaggctt tgtctcgcac ctcaaactcc cccactacgt cgatttccag
     6781 gccgaactgg agctactcaa acgtctgcaa caggagaaga accatggcta atctgagcgg
     6841 ctacaacttt gcctacctcg acgagcagac caaacgcatg atccgccgcg ctatcttaaa
     6901 agcggtggcg atccccggtt atcaggtgcc gtttggcggg cgcgagatgc cgatgccata
     6961 cggctgggga accggcggca tacagctcac cgccagcgtg attggcgaaa gcgacgtgct
     7021 gaaggtgatt gaccagggtg cggatgacac caccaacgcc gtgtcgattc gcaacttctt
     7081 taagcgcgtg accggggtaa acaccactga acgtacggac gatgcgacgc ttatccagac
     7141 gcgtcaccgc atccccgaaa cgccgctgac cgaagatcag atcattatct tccaggtgcc
     7201 aatcccggaa ccgctgcgct ttatcgagcc gcgcgaaacg gaaacccgca ccatgcacgc
     7261 gctggaagag tacggcgtga tgcaggtgaa actgtatgaa gatatcgccc gcttcggtca
     7321 tatcgccact acctatgcct atccggtgaa ggtgaacggg cgctacgtaa tggacccgtc
     7381 gccgatcccg aaattcgata acccaaaaat ggacatgatg cccgccctgc aactgttcgg
     7441 cgcggggcgc gagaagcgca tctatgcggt gccgccgttt acccgcgtgg aaagtctcga
     7501 tttcgacgat cacccgttca ccgttcagca gtgggatgag ccatgcgcca tctgcggatc
     7561 gacccacagc tatcttgatg aagtggtgct ggatgacgcc ggaaaccgca tgtttgtctg
     7621 ctccgatacc gattattgcc gccaacagag cgaggcaaaa aaccaatgaa tcaaccgtta
     7681 ctttcggtca ataacctgac ccacctttac gcgccgggca aaggctttag cgatgtctct
     7741 tttgatttat ggccggggga agtgctgggc attgtcgggg agtccggctc cgggaagacc
     7801 acgctgctga aatcaatctc cgcgcgcctg acgccgcagc agggggagat tcactatgag
     7861 agccgttcgc tgtacgcaat gggcgaggcc gaccgccgtc gcctgctgcg caccgaatgg
     7921 ggcgtagtgc atcagcatcc actcgacggc ctgcgccgtc aggtctcggc aggcggcaat
     7981 atcggcgagc ggctgatggc gaccggggca cgtcattacg gcgatattcg tgccaccgcg
     8041 cagaagtggc tggaagaggt ggaaattccc gccaaccgga tcgacgacct gccgaccacc
     8101 ttttccggcg gcatgcagca acgtttgcag atcgcccgca acctggtgac gcatccgaag
     8161 ctggtgttta tggacgagcc gaccggcgga ctggatgtgt cggtacaggc acgcctgctc
     8221 gacctgctgc gcggcctggt ggtggagctg aacctcgcgg tagtgattgt cacccacgat
     8281 ttaggcgtcg cccgcctgct ggcggaccgt ttgctggtga tgaagcaggg gcaagtggtg
     8341 gagagtgggt taaccgaccg cgtgctcgac gacccgcacc atccgtatac acaactgctg
     8401 gtgtcatcgg ttttgcagaa ttgagccggt gccggatgcg gcgtaaacgc cttatccggc
     8461 ctacaaatgc gctccccgta ggtcggataa gacgcgtcag cgtcgcatcc gacacccgaa
     8521 ccacgaggcg aaaaatgatt aacgtacaaa acgtcagtaa aaccttcatc ctgcaccagc
     8581 aaaacggcgt gcgcctgccc gtcttacaac gtgcctcgct caccgtcaac gcgggcgaat
     8641 gcgtggtgct ccacggccat tccggcagcg gcaaatcaac cttgctgcgc tcgctgtacg
     8701 ccaactatct gcccgacgaa gggcaaatcc agatcaaaca cggtgacgaa tgggtcgatc
     8761 tggtcaccgc gccagcgcgc aaagtggtgg agatccgcaa aaccaccgtc ggctgggtga
     8821 gccagtttct gcgcgtcatc ccgcgtattt cagcactgga agtggtgatg cagccgctgc
     8881 tcgataccgg cgttccgcgt gaagcctgcg ccgctaaagc cgcgcgtctg ctcacccgcc
     8941 tgaacgtgcc ggaacgcctg tggcacctgg cgccatcaac cttttccggt ggcgaacagc
     9001 agcgcgtcaa catcgcccgc ggctttatcg tcgactaccc cattctgctg ctcgacgaac
     9061 ctaccgcctc gctggacgcc aaaaacagcg ccgccgtagt cgagctgatt cgcgaagcca
     9121 aaacccgtgg cgcggccatc gtcggcatct tccatgacga agccgtacgt aatgacgtcg
     9181 ccgaccgctt gcacccaatg ggagcctctg catgattatc aataacgtca aactggtgct
     9241 ggaaaacgaa gtggtacacg gttcgctgga ggtgcaggat ggcgaaatcc gcgcctttgc
     9301 cgaaagccag agccgcctgc cagaagcgat ggacggcgaa ggtggctggc tactacccgg
     9361 cctgattgag ctgcataccg acaacctcga taaattcttc accccgcgcc cgaaagtcga
     9421 ctggcccgcc cattcggcga tgagcagcca cgacgcgctg atggtggcaa gcggcatcac
     9481 caccgtactg gacgccgtgg cgattggcga cgtgcgcgac ggcggcgatc ggctggagaa
     9541 tctggagaag atgatcaacg ccatcgaaga gacgcaaaaa cgcggcgtca accgcgccga
     9601 gcaccgcctg cacctgcgct gcgaactgcc gcatcacacc acactgccgc tgtttgaaaa
     9661 actggttcag cgcgaaccgg tgacgctggt gtcgctgatg gaccactcac cgggccagcg
     9721 ccagttcgct aaccgcgaga agtatcgcga atattatcag ggcaaatact ccctcaccga
     9781 tgcgcagatg cagcagtacg aagaagagca attggcgctc gccgcacgct ggtcgcagcc
     9841 gaatcgcgaa tccatcgccg ccctgtgcca cgcgcgacaa attgcgctcg ccagccacga
     9901 tgacgccacg cacgcccacg ttgccgaatc ccaccaactt ggcagcgtga tcgccgaatt
     9961 tcccaccacg ttcgaagcgg cggaagcctc gcgtaagcat ggcatgaacg tgctgatggg
    10021 tgcgccaaat atcgtgcgtg gcggctcgca ctccggcaat gtggcggcca gtgaactggc
    10081 gcagcttggt ctgctggata tcctctcttc cgactactac cccgccagcc tgctggatgc
    10141 ggcgttccgc gtcgccgatg acgagagcaa ccgctttacg ctaccgcagg cggtgaggct
    10201 ggtgaccaaa aatccggcgc aggcgctgaa tcttcaggat cgcggggtga ttggcgaggg
    10261 taaacgcgct gacctggtgc tggcgcatcg ccagggcaat cacattcata tcgaccacgt
    10321 ctggcgtcag ggtaaaaggg tgttctgatg atgggaaaac tgatttggtt aatggggccg
    10381 tccggctccg ggaaagacag cctgctggcg gaactccgcc tgcgggaaca aacgcagtta
    10441 ctggtggcgc atcgctacat cacgcgcgac gccagcgccg gaagtgaaaa ccatatcgcc
    10501 ctgagtgagc gggagttttt tacccgcgcg gggcaaaacc tgctggcctt aagctggcac
    10561 gctaacggtc tgtattatgg cgtcggcatc gagattgacc tctggctgca cgctggattc
    10621 gacgtagtcg tcaacggttc acgcgcgcat ttgccgcagg cacgggcgcg ctatcaatcg
    10681 gcgctgctgc ccgtctgttt acaggtttcg ccggagatcc tgcgccagcg cctggaaaac
    10741 cgtggccgtg aaaacgccag tgaaattaac gcccggctgg cgcgcgccgc ccgctatact
    10801 ccacaggatt gccatacgct caacaatgac ggcagcctgc gccagtcggt cgacacactg
    10861 ctgacgctga ttcatcagaa ggagaaacac catgcctgct tgtgagcttc gcccggccac
    10921 gcagtacgat accgacgcgg tttacgcgct gatttgtgag ctaaaacagg cggagtttga
    10981 ccaccacgcg tttcgcgtgg gctttaacgc caacctgcgc gacccgaaca tgcgctacca
    11041 tctggcgctg ctggatggcg aagttgtcgg catgatcggc ctgcatttgc agtttcatct
    11101 gcatcatgtc aactggatcg gcgaaattca ggaactggtg gtgatgccgc aggcgcgcgg
    11161 cctgaacgtc ggtagcaagt tgctggcgtg ggcagaagaa gaagcccgcc aggccggggc
    11221 cgaaatgacc gagctttcga ccaacgtgaa gcgccacgac gcgcaccgtt tctatctgcg
    11281 agaaggctac gagcagagcc acttccgctt caccaaggcg ctgtaacatg agcctgaccc
    11341 tcacgctcac cggcactggc ggcgcacagg gcgttccggc atggggctgc gagtgcgcgg
    11401 cctgcgccag agcgcggcgc tcgccgcagt atcgccgcca gccatgcagc ggcgtagtga
    11461 agtttaacga cgcgatcacc ctgatcgacg ccgggcggca cgatctcacc gatcgctggt
    11521 cgcctggatc gttccagcag tttttgctga cgcactacca tatggatcac gtccaggggc
    11581 tatttccgct acgctggggc gtgggcgatg tgatcccggt ctacggcccg ccggatgaac
    11641 agggctgcga cgatctgttt aaacatccgg gcctgcttga tttcagccac acagtggaac
    11701 cgtttgtggt gtttgatttg caggggttgc aggtcacgcc gctgccgctt aaccactcaa
    11761 aactgacctt cggttatctg ctggaaacgg cacatagccg ggtggcgtgg ctgtctgaca
    11821 ccgctggcct gccggaaaaa acgctgaaat ttttactcaa taatcatccg caggtgatgg
    11881 tgattgattg cagtcacccg ccgcgcgcgg atgcaccgcg taatcactgt gatttaaata
    11941 ccgtgcttgc gctgaatcag gttatccgct cgccacaggt gattctgacc catatcagcc
    12001 accagtttga tgcgtggctg atggaaaacg cactaccgtc agggtttgag gtggggtttg
    12061 atgggatgga gattggggtg gcgtgatgag agggaatgtg cgcgctggcc ccctcaccct
    12121 aaccctctcc ccagaggggc gaggggaccg attgtgctca atattgaata ttgcgctcgt
    12181 tttctccctc tccccattgg ggtgaggggc gatgcctgct ccatacccaa cctcatcgcc
    12241 catactcatc ttccattctc cgctcttcat cctccagttg ccgacgctcc tgatcaagct
    12301 ggcgctggcg atcgtccagc tgcctgcggc gatcttcaaa ctggcggcgg cggtcgtcat
    12361 attgtctgcg ccgatcgtcg ctcacttcac gctgccagcc gtcgtcgcgc gaatcttcat
    12421 agtctcgccc acggtcaggg ttataagcgt cattaatcgc ctgctgaata ttaccaatag
    12481 tatcgtcgat aatatcagcc tgggccggaa cggtggacag cgtgagcagg gtgaataaaa
    12541 gaaatagcgg aaagcgtttc attagccaac ctcaaaaaga aactctatcc acattagtca
    12601 ttactcatcc atgcaagtag tggatgaatc tcaatttctc cgctgctcta ttgccgtaat
    12661 cgcctccacg cgttgttgat gacgaccgcc ttcgtactgt gcgcccagcc acgcatccac
    12721 aatcattttt gccagttcga ggccaaccac tcgtgaacca aaagccagtg caacttcact
    12781 ggcataatgc ggataatcag tgcgctctga cgacctggtg tctttatcaa tcacctcact
    12841 cattgaccat gaataaaagg ctggcacagc aacacagaat cctgcggtgc cagcgacatg
    12901 accgcccatt gctccggtac gttttttaaa aagaaacacc ggttgcattg ataaattgtg
    12961 ctcagtattt gccgaagtac aatatatata ataaatatat aaaacacctc atattattta
    13021 cagagataat aaattcgcgt aattttcccc cttcagcaca aatgcctaat aaaatggatc
    13081 gttccgctca cacttccact tattaaagtc gcaaattccc aattatgata acgccagctt
    13141 taaatgacgt attctgcatg gattacatat ttacgacaca aatgataaat ggagatttat
    13201 atgaataact ctcggttatt ccgtttgagc aggattgtta ttgcgttaac tgctgccagc
    13261 ggcatgatgg taaataccgc taacgcgaaa gaggaagcga aagccgccac tcaatatacc
    13321 caacaggtta atcagaatta cgccaaatca ttaccgttta gcgatcgtca ggattttgac
    13381 gacgctcagc gtggatttat cgctccgctg ctggatgaag gtattctgcg cgatgctaat
    13441 ggcaaaccat attatcgcgg ggaagattat aaatttgata tcaatgcgcc cgcgcccgaa
    13501 accgttaacc ctagcctgtg gcgtcagtcg cagttaaacg gtatttccgg cctgtttaaa
    13561 gtcaccgaca gaatgtacca ggttcgcggt caggatatct ccaacatcac ctttatcgaa
    13621 ggtgataccg ggattattgt catcgacccg ctggtgacgc caccgagcgc aaaagccgcc
    13681 cttgaccttt acttccaaaa tcgcccgcaa aaaccgattg ttgcggttat ttatacccac
    13741 agccataccg accactatgg cggcgtgaaa ggcattatct ctgaagctga tgtgaaatcc
    13801 ggtaaagtgc aggttatcgc ccctgctggc tttatggacg aagccatcag cgaaaacgta
    13861 ctggcgggta atattatgag ccgccgtgca ctttactcct acggcctgct gctggcgcat
    13921 aaccctcagg gtaacatcgg caatggtctt ggcgtaacgc tggcatcggg ctacccgagc
    13981 atcatcgcac cgaacaaaac catcaccaaa accggtgaga agatgattat cgacggcctg
    14041 gagtttgact tcctgatgac cccaggtagc gaagcaccag ccgaaatgca cttctatatt
    14101 ccggccctga aagcgctgtg taccgccgag aacgccacgc ataccctgca caacttctac
    14161 actctacgcg gcgcgaaaac ccgcgacacc agcaagtgga ccgagtatct gaacgaaacg
    14221 ctggatatgt ggggtaacga cgcggaagtc ctgtttatgc cgcacacctg gccggtctgg
    14281 ggcaataagc atatcaatga ttatattggt aaatatcgcg atactatcaa gtacattcac
    14341 gaccagaccc tgcacctggc gaaccagggc tacaccatga atgaaatcgg cgacatgatt
    14401 aaactgccgc ctgcacttgc caataactgg gccagccgtg gctattacgg ttctgtcagc
    14461 cacaacgccc gcgcggtgta taacttctat cttggctatt acgacggtaa cccggctaac
    14521 ctgcatccgt atggtcaggt ggagatgggt aaacgttacg tgcaggcgct gggcggttct
    14581 gcccgtgtca tcaacctggc gcaagaagcg aacaagcaag gtgattaccg ctggtcggca
    14641 gaactgctga aacaggtgat tgccgccaac ccgggtgacc aggtcgcgaa gaatctgcaa
    14701 gcgaataact ttgaacagct gggctatcag gccgagtccg ccacctggcg cggtttctac
    14761 ctgaccggcg cgaaagagct gcgcgaaggg gtgcataagt tcagccacgg caccaccggt
    14821 tccccggaca ccattcgcgg gatgtcggtc gaaatgctgt tcgactttat gtccgttcgc
    14881 ctcgatagcg cgaaagccgc gggtaaaaat atcagcctga acttcaatat gagcaatggc
    14941 gataacctca acctgacgct gaacgatagc gtgcttaact accgtaaaac actgcaatcc
    15001 caagctgacg cctctttcta catcagccgt gaagatctgc acgccgtgct gaccgggcaa
    15061 gccaaaatgg cggatctggt aaaagcgaag aaagccaaaa ttattggcaa tggcgcgaaa
    15121 ctggaagaaa ttatcgcctg tctggataat ttcgatttgt gggtgaatat cgtaacccca
    15181 aattaatgtc actcgcttt
//
LOCUS       DAZFWL020000114        15170 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFWL020000114 DAZFWL020000000
VERSION     DAZFWL020000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.114"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            226..882
                     /gene="pphA"
                     /locus_tag="ACHKNP_RS18490"
                     /old_locus_tag="pgaptmp_003698"
     CDS             226..882
                     /gene="pphA"
                     /locus_tag="ACHKNP_RS18490"
                     /old_locus_tag="pgaptmp_003698"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU4705791.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(883..1074)
                     /gene="yebW"
                     /locus_tag="ACHKNP_RS18495"
                     /old_locus_tag="pgaptmp_003699"
     CDS             complement(883..1074)
                     /gene="yebW"
                     /locus_tag="ACHKNP_RS18495"
                     /old_locus_tag="pgaptmp_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HGU4705792.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1179..1415)
                     /gene="yebV"
                     /locus_tag="ACHKNP_RS18500"
                     /old_locus_tag="pgaptmp_003700"
     CDS             complement(1179..1415)
                     /gene="yebV"
                     /locus_tag="ACHKNP_RS18500"
                     /old_locus_tag="pgaptmp_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HGU4705793.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1533..2972)
                     /gene="rsmF"
                     /locus_tag="ACHKNP_RS18505"
                     /old_locus_tag="pgaptmp_003701"
     CDS             complement(1533..2972)
                     /gene="rsmF"
                     /locus_tag="ACHKNP_RS18505"
                     /old_locus_tag="pgaptmp_003701"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HGU4705794.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQATAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3052..5685)
                     /gene="yebT"
                     /locus_tag="ACHKNP_RS18510"
                     /old_locus_tag="pgaptmp_003702"
     CDS             complement(3052..5685)
                     /gene="yebT"
                     /locus_tag="ACHKNP_RS18510"
                     /old_locus_tag="pgaptmp_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HGU4705795.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5654..6937)
                     /gene="yebS"
                     /locus_tag="ACHKNP_RS18515"
                     /old_locus_tag="pgaptmp_003703"
     CDS             complement(5654..6937)
                     /gene="yebS"
                     /locus_tag="ACHKNP_RS18515"
                     /old_locus_tag="pgaptmp_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HGU4705796.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7067..7564
                     /gene="msrC"
                     /locus_tag="ACHKNP_RS18520"
                     /old_locus_tag="pgaptmp_003704"
     CDS             7067..7564
                     /gene="msrC"
                     /locus_tag="ACHKNP_RS18520"
                     /old_locus_tag="pgaptmp_003704"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HGU4705797.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7661..8359
                     /gene="proQ"
                     /locus_tag="ACHKNP_RS18525"
                     /old_locus_tag="pgaptmp_003705"
     CDS             7661..8359
                     /gene="proQ"
                     /locus_tag="ACHKNP_RS18525"
                     /old_locus_tag="pgaptmp_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HGU4705798.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8379..10427
                     /gene="prc"
                     /locus_tag="ACHKNP_RS18530"
                     /old_locus_tag="pgaptmp_003706"
     CDS             8379..10427
                     /gene="prc"
                     /locus_tag="ACHKNP_RS18530"
                     /old_locus_tag="pgaptmp_003706"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HGU4705799.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPDPVK
                     "
     gene            10619..11500
                     /gene="htpX"
                     /locus_tag="ACHKNP_RS18535"
                     /old_locus_tag="pgaptmp_003707"
     CDS             10619..11500
                     /gene="htpX"
                     /locus_tag="ACHKNP_RS18535"
                     /old_locus_tag="pgaptmp_003707"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HGU4705800.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(11546..12919)
                     /gene="yebQ"
                     /locus_tag="ACHKNP_RS18540"
                     /old_locus_tag="pgaptmp_003708"
     CDS             complement(11546..12919)
                     /gene="yebQ"
                     /locus_tag="ACHKNP_RS18540"
                     /old_locus_tag="pgaptmp_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4705801.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPARSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLKPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            13096..13887
                     /gene="kdgR"
                     /locus_tag="ACHKNP_RS18545"
                     /old_locus_tag="pgaptmp_003709"
     CDS             13096..13887
                     /gene="kdgR"
                     /locus_tag="ACHKNP_RS18545"
                     /old_locus_tag="pgaptmp_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HGU4705802.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(14030..14269)
                     /gene="yobH"
                     /locus_tag="ACHKNP_RS18550"
                     /old_locus_tag="pgaptmp_003710"
     CDS             complement(14030..14269)
                     /gene="yobH"
                     /locus_tag="ACHKNP_RS18550"
                     /old_locus_tag="pgaptmp_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HGU4705803.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            14428..14571
                     /gene="mgrB"
                     /locus_tag="ACHKNP_RS18555"
                     /old_locus_tag="pgaptmp_003711"
     CDS             14428..14571
                     /gene="mgrB"
                     /locus_tag="ACHKNP_RS18555"
                     /old_locus_tag="pgaptmp_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HGU4705804.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14646..14933
                     /gene="yebO"
                     /locus_tag="ACHKNP_RS18560"
                     /old_locus_tag="pgaptmp_003712"
     CDS             14646..14933
                     /gene="yebO"
                     /locus_tag="ACHKNP_RS18560"
                     /old_locus_tag="pgaptmp_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HGU4705805.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
BASE COUNT         3984 a         3673 c         3876 g         3637 t
ORIGIN      
        1 taaaagatga gcataaattt atttaacaca ttaattctaa aagattttgt tgtttgttga
       61 cgaaaacagg aatcgtgttc ggtctctttt tatctgttaa aagccagaag catttccttc
      121 gctgacttta tagtcaacca taacacacac tctactgtct gagtccagcg ttttttaaca
      181 ttcttgttaa gattatgtga tctttagcgc gggaggaaaa tattgatgaa acagcctgcg
      241 cccgtttatc agagaattgc gggtcatcaa tggcgacata tctggctttc tggcgatata
      301 cacggttgtc ttgagcagtt gcgccgcaaa ttatggcatt gtcgttttga tccgtggcga
      361 gatttactta tctcagtagg agacgttatc gatcgtgggc cgcaaagttt acgttgtctg
      421 cagttactgg aacaacattg ggtttgtgcg gtaagaggca atcatgaaca gatggcgatg
      481 gatgcgctgg catcccagca gatgtctttg tggttgatga atggcggcga ctggtttatt
      541 gcgctggcag ataatcaaca gaaacaagcg aaaacggcgc tggaaaaatg tcagcatttg
      601 ccctttattc ttgaagtaca cagtcgtacc ggcaaacatg ttattgctca tgccgattat
      661 ccagatgatg tttatgaatg gcaaaaggac gttgatttgc atcaggtctt gtggagccgc
      721 tcgcgattag gtgaacgcca aaaagggcag ggaattacag gtgctgatca tttctggttt
      781 ggtcatacac cgttgcgaca tcgcgtggat attggcaacc tgcattatat tgataccggt
      841 gctgtctttg ggggcgaact gactcttgtg caattgcaat aattaaaaat caccatactc
      901 ctgtgctggt cgccagaaac catctataaa atcctcaatc ggaaaacaac cgccatggcg
      961 gatccgttga tcgctcatag aataaagaca ctgctgttcc gtgttgtaga catccacaac
     1021 aatatcttca caaccgccat ccaggtagca aacaaaaagt accagcgcga acatttcatc
     1081 cccgaagtgt ggtgccgtac cgttaagttt aggagagatt ttacaacggg ggaataacca
     1141 ggacaaataa cccgccatgc cgacgggttc tttttggatc aggcaagacg cataatccag
     1201 gcatcagatt gttgatcgta acgggtacgg taaaggacag agtgttcgcc atttagcagg
     1261 gcagggatac tggtttcagc atcccaggca tcgagttgca tacataaatc ggggtcagat
     1321 ttacaaggaa tggttaacgt tcgatcaccc ggcgattcac cgtgtaattc gccgtcgtcg
     1381 atttcaaaag cgcctatgcg cacactggtt ttcatcttcg tgctctccat ttagctgccg
     1441 attgtggtac gaccagtccg atcgcaccat ttttcagcct agttgaggca gccgaatgtg
     1501 ccagtcaaaa agtgcgcttt ttttgtgagc tgtcaggcgt taccggtaaa aagtttccca
     1561 tcgcgcacca gttcacgcgg atagctgttt ttcaatcgcg aaccaatccg tttggctaaa
     1621 ccaatcggtt gatgctggaa agtaaccaat acatcatccg ccactggcgc ggcttgcggg
     1681 taaacatcgc gcccgcgata ccactcctcc gcttcctgcg gtgtcagctc gaatgcattc
     1741 atattatcgg gtgaggcaag agcaataacc gcttcatgct gccagcgata acctttgttg
     1801 tgcgtctcgg caagtttaat ccccaaccga gaaaatcgga ctttaccgat cagggcttca
     1861 atgcccaccg ggaacaacca cagttctttg tcacgctgcc agaggcgcag gttttcatcc
     1921 cagtttaagc caacacctgc agtcgcctga cgaatttgtc cagcttcgcg atctttcacc
     1981 gggctgaacg gaaaattacc gactttgtat ttgggggcgg gtaaggcggg aatcgcctga
     2041 gttttacgca gacgagcaac gaagaagcct tcgcagtcgt aaatttgtgg gaaaacatgc
     2101 aaaaagcctt cttcggtcag cgctttgttt gcaccaggga agagatcgcc aagtggtaaa
     2161 aactctactg cgtcggggta agtctctttc agccacaggc aaacggcttc gttttcttcc
     2221 tggtttaagg tacaggtcga gtaaaccagc gtaccaccag gacgtaatgc atgaaaggcg
     2281 ctgtcgataa gctcccgttg tgtagctgcg atttcctgat tgctttctgg tgaccagttt
     2341 tttagcgcat cgggatcttt acgcaccacg ccttcgccag agcagggagc gtccagcaaa
     2401 atggcatcga acatttctgg cactgccgca ccaaacacgc ggccatcaaa atgtgtgagc
     2461 gcaacattac tgatgccaca gcggctgata ttggcatgta acacttttac ccgactggcg
     2521 gaaaactcat tggcaaggat tgccccttcg ttattcatcc gcgcggcaat ttgcgtcgtt
     2581 ttggagcctg gcgcagctgc gacatccatc acccgctgtg gtgcattatc gtcagcaaac
     2641 aaggcggcaa cgggcaacat tgaactggct tcctgaatat aaaacaggcc gcttaaatgc
     2701 tcggcggtac tacccaatgg caatgcatct tcattgtcgc gttcaatcca gaaaccttct
     2761 tcacaccacg gaattggcgt aagcgtccag ccataaggag cggttaattg caggaaatca
     2821 gcaacggaga ttttcagcgt attaacgcga atgctgcggc gcaacgggcg ctgacaggcg
     2881 gcaagaaaat catcaaatga gagcgtcgaa ggcatcgctt cgcgcatttg tgtcagaaag
     2941 gcgtccggga aataaacggt gtgttgggcc acgagcatgt accactggca aaaaaacagg
     3001 cgcgcagttt agcataaacg ctccggcgca ggcacgccgg agcagtgggc attatttggg
     3061 aagcgcagtt ccccattcac gccactcttt cggttcactt tcctgcaaca ggaagtgttt
     3121 gccttcctgg gctttcggtg ccagtggcgt acccggaggc gtggcgaagg cgatgccgcc
     3181 acggataaac tggttaaagg tgccggtttt cactacgccg cccgtcagac caaagtccag
     3241 actgtaaccc gatgccaacc agaagacgga attgttacgc accaggtgtt gatagcgttt
     3301 actgatgcgc atcgcaatca tcacgcgatc tgacaatgtc cccagcgtca ttcctgtaac
     3361 cgtaccgact tccagaccac ggaacagcac aggcgtacca atacctaacg aaccggcttc
     3421 cggcgcttca acaataatgc ttaagccatc caggtaacgc gaatcagtaa tggtggcctc
     3481 ttgtaattca aagtcgcggc gaggattgcc ccggcctggt tcgacgttga tatacggctg
     3541 gaggatagta tctagatgct caacgcccgc ggctgaaatt tgcggtgtga ccaccgagaa
     3601 gcgcgtaccg ccgcgggcga aggtctggac atactccgga tagagcaccg cctttgcctg
     3661 tacttcattg cgcgcggtaa tcagatccag cgtctggatt tgcccgatat caataccgag
     3721 atagcgaatt ggcatcccga ccgccagttt tccggcatcg aaagcgtgaa gcgtaatctg
     3781 cccaccaacc gcacgggccg ctgtttcgga agcatacaga attcgtttgt cgcctttacg
     3841 ctgactggcg ctggcaccgc tgaggttatc gaagctaatg gctcccttta atgctctgga
     3901 gagcggggat gcctgtacgg tcaggccact accattcagc tgaactttcg ccccgccttc
     3961 tgcccagaac acgctattgc tggtcagaag gttgcgatac tccggcttaa tatgcagatc
     4021 gatatcaaac gcgttagctc gcggacggac ggtaataact tcaccaactt caaatttacg
     4081 gtagagcact accgatcctg cctgcacatc cggcagcgtc tctgcactca aactcacggt
     4141 tgtggtgggt aaatcgctaa ggctgttctc cagcgctttt tccagattgg catacagtgg
     4201 atagctggct ttcatctcgc ctttatcgcc cggcagaata cgtatcccgc cgttaatcca
     4261 ttctgaggcg ctggcaccga gaaactcaac gccatccagc cccaccttca cgtcgacacg
     4321 gctgttgacg acaaatttgc tatcgccttt taccagttct cgatgctgag gctcgatggc
     4381 gacggtaaag gtgacgcctt tgctggtgag tttacgatcg ataacctggc ctacctgcac
     4441 gccgtgaaga atgagcggct gaccagcatc aataccgtaa ctttccggcg cggtcagggt
     4501 cagcgtcaga acatcaggtt catgcagcag tgctttttcg cctggcacaa caacgaactc
     4561 tttgcgtggc tcgccatcgc cgggtaccaa ctcgaaggtt ttgccagtca gcagggcgct
     4621 gagattggca tcgctaaggg ataatttcgg attacgtaat tcgatgcggg tattttcacg
     4681 aagcagggta acgacgctgg gatcaactgt catttcccca gtgactttac caccaggatt
     4741 taaatccagt ttagttagct gtccgacttc cagcccctga tacattaacg gcgtcgagtc
     4801 ggcggtcaat ccggccccac tcggcagttc cagttttatt attacgccac gctggctgtg
     4861 ggccagatct tcatacagac caaaggtatc ttccgcctcg gcaggtttcg actcttcagg
     4921 tgaatcgaag gcaatcgcac cgttaaccag tgccgccaga ctttccagtt tcaccttcgc
     4981 gccactgaca ctgacgttgg catcaacgcc ggaaacgttc cagaaacggc tacctttttt
     5041 caccagatcg gtaaaacgcc gctcgatcag aacatcaatc accacgcctt gcttgttggg
     5101 attgatggca tagtcgtaga cttttcccac cgggatcttg cggaaataga ccaatgaacc
     5161 gctgttcagc gaaccgagat cgggagcttg caggtggatc atcagatcgc cgttatccag
     5221 ccgatacttc ggttgggtat cgagtgcgac aaagtgatcc tgctctttac ctttacccgg
     5281 catcatgccg atatagttcc caccgacgag ggcgtccagc ccggagacac ctgccaacga
     5341 tgcttttggc gtcaccagcc agaattgagt ctcttcgcgc agcgcatctt tcatatcgga
     5401 cttgatgctg accttgactt caatcttacg aagatcgtcg ctgaggctga tatcctgcac
     5461 tgttccgact tcaacgccct gataacgaac aggcgtacgg cccggaacaa taccatccgc
     5521 cgacataaag tcgatggtga cggtattacc ccggtcctga taactgtccc aaatcagcca
     5581 accggcaatc attagcgcga tgaaaggcag cagccagaaa ggtgagatac ggcgtttatt
     5641 tttaatctgc gcttcagtcg tcgaagcggg cgtttcctga ctcatgtgca tcccaaagta
     5701 agcggctgtc cagccattcc acagcaagaa tagtcaaaat tacggctgcg ccaaaataaa
     5761 acgcagccgg tcccatagta aaagcgagga tctgatcgcg attaatcagc gacatggtta
     5821 aagatatgac aaacaggtcg agcatcgacc agcgtccaat ccaggtcacc atccgcagta
     5881 acagaatgcg tgtgcgtaaa ccttgctggc atttaaaatg aatgctcaac agtaaagtga
     5941 acatgacgat cactttagta aacggtacca gaatactggc gataaacacg attcccgcaa
     6001 ccgcaatgtt gctacttgcc agcgacataa ttccggaaag aattgtatct tcctgccgtc
     6061 ctccgttcag ataaatgata gaaataggca ataggttggc tggtaacaac aaaacgattg
     6121 acgctaacag cgccgcccag catttttgca gactatgacg gcgacgcagg cgtagcggga
     6181 tatggcaacg cgggcagcga ccacgctgat caggatagcc ggtaaaatgg cacccaagac
     6241 agacacgaag tttctcgtcc ctacgcgtag cggggcgctg cggataatat cgctcccaca
     6301 gctcctcaac attaagatgt gacaacgtca ccgtcgttaa aatcaccaac gccacaaaag
     6361 agaacaagcc gacaccagcc tggatatggg cataatcctg tacctttata gaagcaacgc
     6421 caatgccgac caggtagata tccagcatta cccactcttt aagacgctca agcatcagca
     6481 gtaccggacg taaattcatt cccagtcggt taccaaacca taaatatgct atggaggaca
     6541 ccagaatgag gggggcacca ataacgcaga aaaagaccat cgcccccgtt atcgtatcgc
     6601 cctgtttggt catttgccag atgccttgca tgacgttggc gtcgatacga atacctaaca
     6661 gccagatatg caacagcggt tcgccccagg caaacggcat cagcaacagc atggtgaaag
     6721 ccattgccgc caggcgcgtt agcgaccagt cgcgcccgtc gcgaattttt gcctgacagc
     6781 gcggacaata ggcactctga tgagaattta tctcgggcag gctaaacagc atgtcacatt
     6841 gcgggcaacg ttggtaatca ccacgcggca gttcctcgcc gattgccctc actgttatct
     6901 tttttgtcgg cgtaatttgt ggtgtgttaa gagccatgac gtgccaaatg taataagaat
     6961 taacattaat attaacccat gattggaatg atcttgagca ttaacgcatt taatgcttat
     7021 tttaataggc tggcagttcc aggtaagaca accaagtgat tatataatga acaaaacaga
     7081 attttacgcg gatttaaatc gcgactttaa cgcgctgatg gcgggagaaa ccagttttct
     7141 ggcaacgctt gcgaacacca gtgcgttgtt atacgagcgt ctcactgacg taaactgggc
     7201 aggtttttat ttgcttgagg acgatacact ggtactagga ccatttcagg gcaaaattgc
     7261 ctgtgtccgg atacccgtcg ggcgcggggt gtgcggcact gcggttgccc gcaatcaagt
     7321 gcagcgtatc gaggatgttc acgcgtttga cgggcatatt gcctgtgatg cggcgagtaa
     7381 ttctgaaatt gttctgccgc tggtggtgaa aaatcagatt attggtgttc ttgacatcga
     7441 tagtaccgtc ttcggtcgct ttacagacga ggacgagcaa ggcttacgtc agcttgtggc
     7501 acagcttgaa aaagtgcttg caacgacgga ttacaaaaaa ttctttgcga gcgtcgcagg
     7561 ataatcaacg gataacgtag catttactgg tagcgtcatt ataatgacgc ctgttcatgc
     7621 ctgcgcttgt tggctacgtc cgttgtaatc aggaaatttc atggaaaatc aacctaagtt
     7681 gaatagcagt aaagaagtaa tcgcgtttct ggccgaacgt tttccccact gtttcagtgc
     7741 ggaaggtgaa gcgcgtccgc tgaaaatcgg tatttttcag gatttggtcg atcgtgttgc
     7801 cggggaaatg aacctgagca aaacgcaatt acgatccgct ttacgtctct acacttcgag
     7861 ctggcgttat ctttacggtg ttaaacccgg cgcaacgcgt gtcgatcttg acggcaaccc
     7921 atgcggtgag ctggacgagc aacatgtaga gcatgctcgc aagcagcttg aagaagcgaa
     7981 agcgcgtgtt caggcacagc gtgctgaaca gcaagcgaaa aaacgcgaag ctgccgcagc
     8041 tgctggtgag aaagaagacg caccgcgccg cgaacgcaag ccacgtccga ccacgccacg
     8101 ccgcaaagaa ggcgctgaac gtaaacctcg tgcgcaaaag ccggtagaga aagcgccaaa
     8161 aacagtaaaa gcacctcgcg aagaacagca caccccagtt tctgacattt cagctctgac
     8221 tgtcggacaa gccctgaagg tgaaagcggg tcaaaacgcg atggatgcca ccgtattaga
     8281 aatcaccaaa gacggcgtcc gcgtccagct gaattcgggt atgtctttga ttgtgcgcgc
     8341 agaacacctg gtgttctgaa acggaggccg ggccaggcat gaacatgttt tttaggctta
     8401 ccgcgttagc tggcctgctt gcaatagcag gccagacctt cgctgtagaa gatatcacgc
     8461 gtgctgatca aattccggta ttaaaggaag agacgcagca tgcgacggta agtgagcgcg
     8521 taacgtcgcg cttcacccgt tctcattatc gccagttcga cctcgatcag gcattttcgg
     8581 ccaaaatctt tgaccgctac ctgaatctgc tcgattacag ccacaacgtg ctgctggcaa
     8641 gcgatgttga acagttcgcg aaaaagaaaa ccgagttagg cgatgaactg cgttcaggca
     8701 aactcgacgt tttctacgat ctctacaatc tggcgcaaaa gcgccgtttt gagcgttacc
     8761 agtacgcttt gtcggtactg gaaaagccga tggatttcac cggcaacgac acttataacc
     8821 ttgaccgcag caaagcgccc tggccgaaaa acgaggctga gttgaacgcg ctgtgggaca
     8881 gtaaagtcaa attcgacgag ttaagcctga agctggcagg aaaaacggat aaagaaattc
     8941 gtgaaaccct gacacgccgc tacaaatttg ccattcgtcg tctggcgcaa accaacagcg
     9001 aagatgtttt ctcgctggca atgacggcgt ttgcgcgtga aatcgacccg cataccaact
     9061 atctttcccc gcgtaatacc gaacagttca acactgaaat gagtttgtcg ctggaaggta
     9121 ttggcgcagt gctgcaaatg gatgatgact acaccgttat caattcgatg gtggcaggtg
     9181 gtccggcagc gaagagtaaa gctatcagcg ttggtgacaa aattgtcggt gttggtcaaa
     9241 caggcaagcc gatggttgac gtgattggct ggcgtcttga tgatgtggtt gccttaatta
     9301 aagggccgaa gggcagtaaa gttcgtctgg aaattttacc tgctggtaaa gggaccaaga
     9361 cccgcactgt aacattgacc cgtgaacgta ttcgtctcga agaccgcgcg gttaaaatgt
     9421 cggtgaagac cgtcggtaaa gagaaagtcg gcgtgctgga tattccgggc ttctatgtgg
     9481 gtttgacaga cgatgtcaaa gttcaactgc agaaactgga aaaacagaat gtcagcagtg
     9541 tgatcatcga cctgcgtagc aatggcggtg gggcgttgac cgaagcggta tcgctctcag
     9601 gtctgtttat tcctgcgggt cccattgttc aggtccgcga taacaacggc aaggttcgtg
     9661 aagacagcga taccgacggg caggtgttct ataaaggccc gctggtggta ctggttgacc
     9721 gtttcagtgc ttcggcttca gaaatctttg ccgcggcaat gcaggattac ggtcgtgcgc
     9781 tggttgtggg tgaaccgacg tttggtaaag gcaccgttca gcaataccgt tcattgaacc
     9841 gtatttacga tcagatgtta cgtcctgaat ggccagcgct gggttctgtg cagtacacga
     9901 tccagaaatt ctatcgcgtt aacggcggca gtacgcaacg taaaggcgta acgccagaca
     9961 tcatcatgcc gacgggtaat gaagaaacgg aaacgggtga gaaattcgaa gataacgcgc
    10021 tgccgtggga tagcattgat gccgcgactt atgtgaaatc aggcgattta acggcctttg
    10081 ggccggagct gctgaaggaa cataatgcgc gtatcgcgaa agatcctgag ttccagaaca
    10141 ttatgaagga tatcgcgcgc ttcaacgcta tgaaggacaa gcgcaatatc gtttctctga
    10201 actacgctgt gcgtgagaaa gagaataatg aagatgatgc gacgcgtctg gcgcgtttga
    10261 acgaacgctt taaacgcgaa ggtaaaccgg agttgaagaa gctggatgat ctaccgaaag
    10321 attaccagga gccggatcct tatctggatg agacggtgaa tatcgcactc gatctggcga
    10381 agcttgaaaa agccagaccc gcggaacaac ccgatcccgt caagtaatat caatcaggca
    10441 caagaaattg tgcctgattt tttaacagcg gcaagatgcc gtaaatcaga tgctacaaaa
    10501 tgtaaagttg tgtctttctg gtgacttacg cactatccag acttgaaaat agtcgcgtaa
    10561 cccatacgat gtgggtatcg catattgcgt tttgttaaac tgaggtaaaa agaaaattat
    10621 gatgcgaatc gcgctcttcc tgctaacgaa cctggccgta atggtcgttt tcgggctggt
    10681 actgagcctg acagggatac agtcgagcag cgttcagggg ctgatgatca tggccttgct
    10741 gttcggtttt ggtggttcct tcgtttcgct tctgatgtcc aaatggatgg cattacgatc
    10801 tgttggcggg gaagtgatcg agcaaccgcg taacgaaagg gaacgttggc tggtcaatac
    10861 tgtagcaacc caggctcgtc aggcagggat cgctatgccg caagtggcta tctaccatgc
    10921 gccggacatc aacgcttttg caaccggtgc gcgccgtgat gcctctctgg ttgctgtcag
    10981 caccggtttg ctgcagaaca tgagcccgga tgaagccgag gcggtaattg ctcacgaaat
    11041 cagtcacatc gccaatggtg atatggttac catgacgctg attcagggcg tggtgaacac
    11101 cttcgttatc tttatttccc gtattctggc gcagcttgcc gcgggtttta tgggcggaaa
    11161 tcgtgatgaa ggtgaagaga gcaacggcaa cccgctgatc tactttgcgg ttgcaacggt
    11221 tctggaactg gtgtttggta ttctggcgag cattatcacc atgtggttct cgcgtcatcg
    11281 tgaattccat gctgatgccg gttcggcaaa actggttggt cgcgagaaaa tgattgccgc
    11341 gctgcagcgc ctgaaaacca gctatgaacc gcaagaagca accagcatga tggctttctg
    11401 cattaacggt aagtcgaaat cgctcagtga gttgttcatg actcacccgc cgctggataa
    11461 acgaattgaa gctctgcgta cgggtgaata cctgaagtaa tatgaagtaa atactaaaag
    11521 gcgcgtcctg atcgacgcgc tttttttatg ccctggatcg tggctgagtg atacgtaaac
    11581 cactcacaca ggcagcaatc actgccagaa tagccgcagc catcagcgaa acgtgcgtac
    11641 cattatcacc gaactgattt agcatcagcg ccaccagcgc cgcgccacta ctctgaccca
    11701 gtagacgagc cgttcctaac atgccactgg ccccaccgct acgttcgcgc ggagcggagg
    11761 taataatggt gtggttattg ggtgactgga ataagccaaa tccagcgcca cataagatca
    11821 tcggccagat aatattgata tccgcaggtg acgcgggcag cagaaccagg gaaaaaagcc
    11881 ccgcagccat gatgaacaac cctaaagcac ccagcaatcc tgcatgtacg cgttcaatca
    11941 aatagcctgc cagcggagcc atcaccatcg ttgctaacgg ccacggtttc agaagtaaac
    12001 ctgtttcgac ttcactacgc ccgagcacgg tttgcaggta aaagggcagg gaaaccattg
    12061 ccagcatttg tgcgcagaaa gagcaaacag atgtgcaaat agaaagtgaa aacagcggga
    12121 tacgcagtaa atccaccggt agcagcggta cgggaagaga aagctggcgg cgaataaaga
    12181 aaataccaac aacaaccatt accaccagtt ccgcagcaat taacgtcagc gattgcccct
    12241 gagcgaaacc actcaacgca gtgataagca ggccgaaggt taacgcgttc atcacggcgc
    12301 tgggcaggtc gaaacggggt ttactggcgc gagaaccatt gggtggcaga aaacgcatcg
    12361 ccagaagcag ggcgataata cctaacggta cgttgattaa aaataaccat ttccaggatg
    12421 cgatggagag gattgctgca gcaattgtcg gcccggcagc agaagaaacg gcaacaataa
    12481 acgagtttat gcccatccct ctccccagaa aacgttgtgg atagatcagg cggataagtg
    12541 cggtattaac gctcatcaac gctgcaccgc cgaaaccttg tatgacacgc gcaagggtga
    12601 gcatttgcag cgaatcagaa agggcgcaga acagtgaaga caacagaaaa acgaccagac
    12661 cgcatttata aatacgtcga tagccaaaca tatcgcccag aaacgaaaac gagagcaggg
    12721 agatgacaat ggcgatttga taggcgttca ctacccagat ggatctggct ggcgtggcat
    12781 gaaggtccgt ggcgattgtt ggcagggcga cgttggcgat tgcgccgtca aggacagcca
    12841 tcgaaatgcc aatcacaatg gttaatatcg caccgtatcg ctggggcaat ggcaggccgt
    12901 cggcctgaac ttttggcata ggaattttat atctttggtg aataatcaac aggggtatga
    12961 ttctagcact gatattcacg gtggatgtcg cagaattgta actaaccggc aaaagatgga
    13021 ttgcgaccac cctggtgcga aattataata aaaaacagtt ctgattttta taaaacactc
    13081 gcaatgaggt gataaatggc taacgcagat ctggataaac agcctgattc tgtatcttcc
    13141 gtgctaaaag tttttggcat tttgcaggcg ctgggtgaag agcgcgaaat agggataacc
    13201 gagctgtcgc agcgcgtcat gatgtcaaaa agcaccgttt atcgcttttt acagaccatg
    13261 aaaaccttag gttatgtggc gcaggaaggg gagtcggaga aatattccct gaccctgaaa
    13321 ttgtttgaac tgggcgctcg cgcgttacaa aacgtcgatt taattcgtag cgcagatatc
    13381 cagatgcgtg agatctcccg cctgaccaaa gaaactatcc acctcggcgc actggacgaa
    13441 gacagtattg tttacattca caaaattgac tctatgtaca atttgcgcat gtattcacgg
    13501 attgggcgtc gtaatccgct gtacagcacc gcgattggta aggtactgct ggcatggcgc
    13561 gatctcgatg aagtgaagca aattcttgag ggcgtggagt ataaacgcag taccgagcgg
    13621 accatcacca gtacagaagc gttattaccc gttctggacc aggtgcgcga gcaggggtat
    13681 ggcgaagata acgaagagca ggaagaaggg ctgcgatgca ttgcggtacc ggtatttgat
    13741 cgctttggcg tggtcattgc cggtttgagc atctccttcc cgacgttgcg tttctctgaa
    13801 gagcgtttac aggaatatgt cgcaatgttg cataccgcag cgcgcaaaat ttctgcccaa
    13861 atgggttatc acgactatcc gttctgatga gagtaagaac ctgtcggaat atcaaacaga
    13921 caggttcttt atttagcatg agaaaaataa agttgaaggt gcgttatatt aaacgcgctt
    13981 gctataagaa tattttactc atgagtgaga atctggttat ttattaccct taaccattat
    14041 cgaccacgat attgctttta cgtaacagtg ggcaatctgt tatccccaag aaaccacttt
    14101 tagtgtgcaa gtattgtacc gtgctggtgc ctctggcagt cagataggta cattgcaaac
    14161 ctaatcctgc ggcattctct ttgcttccaa tcaaaacgcc atatccgctg agtaataatc
    14221 ctatccatac cagtgctatc agcataactg tgcgaatgat gaatcgcatt acaacctctt
    14281 ctctttttat gttcgcttaa tcgtagcggc aatatgcgct gaagcaagct actcattccg
    14341 aaaaagcacg aatatcgaca tagttaggcg ctgtttaact aacgcatgct agtttaatga
    14401 cataaggtag gtgaagcgga gattggagtg aaaaagtttc gatgggtcgt tctggttgtc
    14461 gtggtgttgg cttgcttgct gctttgggcg caggtattca acatgatgtg cgatcaggat
    14521 gtacaatttt tcagcggaat ttgtgccatt aaccagttta tcccgtggtg atatcatttt
    14581 ttcagcacta gattttcttc ctctccagca gtggtagaat agcgcctcta ctcagaggtg
    14641 gtgaaatgaa cgaagttgta aattcaggcg tgatgaacat tgcgtctttg gttgtatcgg
    14701 tggtggttct tcttatcggg ctcatcttgt ggttttttat caatcgtgcc agttctcgga
    14761 ctaacgaaca gattgaactg cttgaggcgt tgctggatca gcaaaaacgt caaaatgcac
    14821 tgttacgtcg tttgtgcgaa gcaaacgaac ctgagaaagc agataaaaag accgttgaga
    14881 gtcaaaaatc ggttgaagac gaagatatta ttcgtctggt cgccgaacga taatccttcc
    14941 tcaatcttga gtaaccccgt cgttgaaggg ttactcaatt catcctgatt atacgcactg
    15001 ccatggtgca actgcttcaa attaaaactc tggtctgact tcaaataatt atcatattat
    15061 ttatttgcgt aacgggatat cagaaaaaga tattttcctc attaatttat taggatgttt
    15121 acatcggatt tgtgattaag cgtggtatta tttattagcg aaacgtttct
//
LOCUS       DAZFWL020000115        14971 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFWL020000115 DAZFWL020000000
VERSION     DAZFWL020000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.115"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(71..146)
                     /locus_tag="ACHKNP_RS18565"
                     /old_locus_tag="pgaptmp_003713"
     tRNA            complement(71..146)
                     /locus_tag="ACHKNP_RS18565"
                     /old_locus_tag="pgaptmp_003713"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Phe,seq:gaa)
     gene            complement(252..959)
                     /gene="yqgA"
                     /locus_tag="ACHKNP_RS18570"
                     /old_locus_tag="pgaptmp_003714"
     CDS             complement(252..959)
                     /gene="yqgA"
                     /locus_tag="ACHKNP_RS18570"
                     /old_locus_tag="pgaptmp_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HGU4705806.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1112..1195
                     /gene="yqgH"
                     /locus_tag="ACHKNP_RS18575"
                     /old_locus_tag="pgaptmp_003715"
     CDS             1112..1195
                     /gene="yqgH"
                     /locus_tag="ACHKNP_RS18575"
                     /old_locus_tag="pgaptmp_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HGU4705807.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            1357..3492
                     /gene="speC"
                     /locus_tag="ACHKNP_RS18580"
                     /old_locus_tag="pgaptmp_003716"
     CDS             1357..3492
                     /gene="speC"
                     /locus_tag="ACHKNP_RS18580"
                     /old_locus_tag="pgaptmp_003716"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HGU4705808.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3542..4798)
                     /gene="nupG"
                     /locus_tag="ACHKNP_RS18585"
                     /old_locus_tag="pgaptmp_003717"
     CDS             complement(3542..4798)
                     /gene="nupG"
                     /locus_tag="ACHKNP_RS18585"
                     /old_locus_tag="pgaptmp_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HGU4705809.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5000..6079)
                     /gene="mltC"
                     /locus_tag="ACHKNP_RS18590"
                     /old_locus_tag="pgaptmp_003718"
     CDS             complement(5000..6079)
                     /gene="mltC"
                     /locus_tag="ACHKNP_RS18590"
                     /old_locus_tag="pgaptmp_003718"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HGU4705810.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6144..6419)
                     /gene="yggX"
                     /locus_tag="ACHKNP_RS18595"
                     /old_locus_tag="pgaptmp_003719"
     CDS             complement(6144..6419)
                     /gene="yggX"
                     /locus_tag="ACHKNP_RS18595"
                     /old_locus_tag="pgaptmp_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HGU4705811.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6447..7499)
                     /gene="mutY"
                     /locus_tag="ACHKNP_RS18600"
                     /old_locus_tag="pgaptmp_003720"
     CDS             complement(6447..7499)
                     /gene="mutY"
                     /locus_tag="ACHKNP_RS18600"
                     /old_locus_tag="pgaptmp_003720"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGU4705812.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7660..8379
                     /gene="trmB"
                     /locus_tag="ACHKNP_RS18605"
                     /old_locus_tag="pgaptmp_003721"
     CDS             7660..8379
                     /gene="trmB"
                     /locus_tag="ACHKNP_RS18605"
                     /old_locus_tag="pgaptmp_003721"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGU4705813.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8379..8705
                     /gene="yggL"
                     /locus_tag="ACHKNP_RS18610"
                     /old_locus_tag="pgaptmp_003722"
     CDS             8379..8705
                     /gene="yggL"
                     /locus_tag="ACHKNP_RS18610"
                     /old_locus_tag="pgaptmp_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HGU4705814.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            8889..9608
                     /gene="yggN"
                     /locus_tag="ACHKNP_RS18615"
                     /old_locus_tag="pgaptmp_003723"
     CDS             8889..9608
                     /gene="yggN"
                     /locus_tag="ACHKNP_RS18615"
                     /old_locus_tag="pgaptmp_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HGU4705815.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9784..10830
                     /gene="ansB"
                     /locus_tag="ACHKNP_RS18620"
                     /old_locus_tag="pgaptmp_003724"
     CDS             9784..10830
                     /gene="ansB"
                     /locus_tag="ACHKNP_RS18620"
                     /old_locus_tag="pgaptmp_003724"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HGU4705816.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVAAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10947..11954
                     /gene="yggM"
                     /locus_tag="ACHKNP_RS18625"
                     /old_locus_tag="pgaptmp_003725"
     CDS             10947..11954
                     /gene="yggM"
                     /locus_tag="ACHKNP_RS18625"
                     /old_locus_tag="pgaptmp_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HGU4705817.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQAFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(12010..13311)
                     /locus_tag="ACHKNP_RS18630"
                     /old_locus_tag="pgaptmp_003726"
     CDS             complement(12010..13311)
                     /locus_tag="ACHKNP_RS18630"
                     /old_locus_tag="pgaptmp_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HGU4705818.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLVYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(13311..13814)
                     /locus_tag="ACHKNP_RS18635"
                     /old_locus_tag="pgaptmp_003727"
     CDS             complement(13311..13814)
                     /locus_tag="ACHKNP_RS18635"
                     /old_locus_tag="pgaptmp_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HGU4705819.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(13859..14845)
                     /locus_tag="ACHKNP_RS18640"
                     /old_locus_tag="pgaptmp_003728"
     CDS             complement(13859..14845)
                     /locus_tag="ACHKNP_RS18640"
                     /old_locus_tag="pgaptmp_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HGU4705820.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         3969 a         3588 c         3783 g         3631 t
ORIGIN      
        1 ggcaggacgc ttaaatcgtg gcgtcctgaa acgaaaaacg gacctccgtg gaggtccgtt
       61 tatatgaatt tggtgcccgg actcggaatc gaaccaagga cacggggatt ttcaatcccc
      121 tgctctaccg actgagctat ccgggcaacg gggcgcatta aacctgattc gcctcgtctc
      181 gtcaatcaaa tttcttcaat ttactgcaga ctgcacaatc tatcatcact ttgccgctat
      241 tgcacgcatt gtcaggcaaa ccaggcggtc caggcggcgg aaagcggcat tgccagaagg
      301 agtgctggaa gcatgttgac caccgggaac attttaatgc cacagatgcg taatccggtt
      361 gccagcagca ataaaccgcc tacggcgctg aagtctgcca tcatcgacgg tgtggtcagc
      421 ggtaatatca gcgcggcagc ccacgccagc gttaactgga tgatcagtaa tgggatacta
      481 attaccgaca ccgcaatacc cagcgagcag gcgaagatca tcgccgtaaa gaaatcaaga
      541 aatgacttgg cgattaaaat actcggatcg ccggtcatcc cttcgttcat cgccccgaag
      601 atcccggtgc cgctggcgca aaacaggaca ataatcgcga cataattctg aataaaagat
      661 tcatgcgctg gcttcttacg tgagtgacga aacagatttt gtgctttggc gactgctgta
      721 ttgacacctt tctccagcaa acaaatttcg ccaattagag cgccaagtag cgtagctaaa
      781 accatcgctg gaaggttggc gcatttcaca accagtaaaa taccaatccc cagcgatgcc
      841 agaccaaaaa tagatgtcat ggagacgcgg atacgttccg gtaagcgttg gctgagcagt
      901 gcgccgagaa cgccacccag taagactgca ctagcgttga taaaagggcc gatgaccaca
      961 agagttcctg ttagctgtat tgtttgattt tcttattatg aactttttgc cttgttgatg
     1021 gcttgtctgg tcgcaaagtc ctggcttgca cgctttagcg aaaggtgcca tgattgcgcg
     1081 aattttctcc tctctgtacg gagtttgccc gatgcacgcc atctccttac attctctcgc
     1141 ttatcgccgt ttcgcgcgaa acgtttccct ttctatgtta ctgctcatgc ggtgaagtta
     1201 acgcacgctc actgcaggac aacagtaaaa tcagagcgtt tctgctttta ctgatgtctg
     1261 gcggtcggag ctggtgacca gtttgaccca tatctcatgg ggcagggttt tccaccttgt
     1321 ccggtattct tacttccccg aaacgggttt gcgcttatga aatcaatgaa tattgccgcc
     1381 agtagtgaac tggtatcccg actttcttct catcgtcgcg tggtggcgtt gggagatact
     1441 gattttacgg acgtcgcggc agtcgtcatt accgctgcgg atagtcgcag tggcattctt
     1501 gcgttgctta agcgcaccgg ttttcatcta ccggtgtttt tgtattccga acatgctgtt
     1561 gaattacctg cgggcgttac ggcggtaatc aacggcaacg agcagcagtg gctggagctg
     1621 gaatccgcag cctgtcagta tgaagagaat ttgctgccac cgttttatga cacgctgacg
     1681 cagtacgttg agatgggcaa cagcaccttt gcttgccctg gacatcaaca tggtgcgttt
     1741 tttaaaaagc atcctgccgg acgccatttt tacgatttct ttggtgagaa cgtctttcgc
     1801 gccgatatgt gtaacgctga cgtaaaattg ggcgatctgc ttattcatga aggatcggcg
     1861 aaagatgcgc agaaattcgc agccaaagtc tttcatgccg ataaaaccta ttttgtgctg
     1921 aacggcacat cggcagcgaa taaagtggtg acgaatgcgc tgttaacgcg tggcgatctg
     1981 gtgctcttcg accgtaacaa ccataagtcg aatcatcacg gcgcgctgat tcaggcgggg
     2041 gcgacgccgg tctatctgga agcttcacgc aacccgtttg gtttcattgg cggtattgat
     2101 gcgcactgtt ttaatgaaga gtatctgcgc cagcaaattc gcgacgttgc gccagaaaaa
     2161 gccgagctgc cgcgcccgtt tcgcctggcg attattcagc tgggaaccta tgacggaact
     2221 gtctataacg cccgtcaggt gatcgatacc gttgggcatc tgtgtgatta cattctgttt
     2281 gattccgcgt gggtcggtta cgaacaattt atcccgatga tggcggatag ctcgccgctg
     2341 ctgttagaac ttaacgaaaa cgatccgggg atttttgtga cccagtcggt gcacaaacag
     2401 caggctggat tctcacagac gtcgcagatc cataaaaaag ataaccatat ccgcggacag
     2461 gcgcgttttt gcccgcataa gcggttgaat aacgccttta tgctccatgc ttctaccagc
     2521 cctttctatc cgctgtttgc tgcactggat gttaacgcca aaattcatga aggggagagt
     2581 gggcgtcggc tgtgggctga gtgcgttgag ttgggtattg agtcgcgcaa ggctattctt
     2641 gcgcgctgta agctgttccg cccgtttatc ccgcccgttg ttgatggcaa attgtggcag
     2701 gattatccga catctgtgtt agccagcgac cgccgttttt tcagttttga gccgggggcg
     2761 aagtggcacg gctttgaagg atatgccgcg gatcagtatt ttgttgatcc gtgcaagctg
     2821 ttactcacta caccaggtat caatgccgaa actggcgaat atagcgactt tggcgttccg
     2881 gcgacgattc tggcgcacta tctgcgtgag aacggcattg tgccggagaa gtgcgatctc
     2941 aactccattc tgttcttatt aactccggcg gaaagccacg agaagctggc acaactggtg
     3001 gcgatgctgg cgcaatttga acagcatatt gaggatgact cgccgctggc tgaggtgttg
     3061 ccgagcgttt ataacaaata tccggtgcgc tatcgcgact acaccctgcg ccagttgtgt
     3121 caggagatgc acgatctgta tgtcagtttc gacgtcaaag acctacaaaa agcgatgttc
     3181 cgccagcaga gtttcccgtc agtggtgatg aacccccagg atgcgcatag cgcttatatt
     3241 cgcggtgaag tggagttggt gcggattcgt gatgccgaag ggcgaattgc ggcagaaggg
     3301 gcgttgcctt atccacctgg cgtgctttgc gtggtacccg gggaagtctg gggtggggcg
     3361 gttcaacgtt atttccttgc actggaagaa ggggtgaatt tgttgccggg attttcgccg
     3421 gagctgcaag gtgtttatag cgaaaccgat gcggatggca tgaaacggtt gtacggttat
     3481 gtgttgaagt aagaataaaa aaaacgggtc accttctggc gacccgtttt tctttgcgta
     3541 attagtggct aaccgtctgt gtgcctgtcg ggacacgaac gtgtttatat ttgaacatcg
     3601 ccatgaacgc gaaggccaga accacggagt aaccagcgaa aatcaaccat acggtctgcc
     3661 agtcggtaat gccgttttgg gtgtacatct caacaacttt accgctcacg atgccgccga
     3721 ggatacagcc gaagccgtta gtcatcatca ggaacatccc ttgtgcactg gcgcgaattg
     3781 ccgggctaac ttctttttcg acaaacaccg aaccagagat gttgaagaag tcgaatgcgc
     3841 aaccgtaaac gatcatcgac agtaccagca gtacagtacc gaacggagtc gggtcgccgt
     3901 aagcaaacag cgcaaaacgc aggatccacg ccacaatact gatcatcatt acgttcttaa
     3961 taccgtagcg gcttaagaag aacgggatgg tcagaatgaa cagggtttca gagatctgcg
     4021 aaatcgacat gatgattgac gcatgctgca caataaagct gctggcaaac atcggatctt
     4081 tgtcgaagct gtgcaggaag gtattaccga acatattggt aatctgcagt tccgcgccca
     4141 gcagcattga gaagataaag aagattgcca tacgcttgtt tttaaacagc gcgaatgcat
     4201 cgaggcccag cagggttgtc cagctctgat tcgcttgctg tttagcaacc ggaatatgcg
     4261 gcagagtcag ggtaaacaga accagaatgg cggaaagtgc tgcgccaata tacagctgca
     4321 tgtggcttaa ttcgaagcca gacaggctca ccacccacat tgccatgata aagccgatgg
     4381 tgccccagat acggattggc gggaagtcag taacgatatc catcccggca ttttgcaggc
     4441 gatagtaaga gatggtgttg attaacccaa gcgttggcat ataagcaaac gagttaatca
     4501 atatcacaag gaacatcgcc tccggtgtcg tgacctgtgc cgccatgaac agcgtgatag
     4561 cgccaatggt gtggcaaatg gcatataccc atttcgcact taaccatttg tcggccacaa
     4621 tccccagcag cgcaggcata aagaccgcgg cgatacccag tgagctataa actgcgccaa
     4681 tagaagcacc gtcaaacttc agggtaacaa acatatagga gccgagggtc gtcagccaac
     4741 ttccccacag acagaactgc agaaaagaga ggattttcag ctgcagctta agattcatgt
     4801 taatttcctc acatcgtgat gcggatgagt gtttgaaaaa gcacatttga ccgtgcgaga
     4861 tgatgggatt ctatcaactt cgcggactgg ttttttgtta cctgtggcaa ataattgcaa
     4921 acattcccaa tttgcaaaac gaaattgtga tgtggataac atttttgccc ctgagcatcg
     4981 tcaggggcgg ttaatggaat tatcggcggc ggtaggattt ttgcgcggta ttcactttat
     5041 aaagataacg gcgagattcc gcagagggat ggcgggtcgt cagcgtctga taaacatcgc
     5101 ccggcgtcat ggtgttaata atattggcag cctgaatctt atcattcgaa aagactcgca
     5161 gcacgctgcc tgcgccgccg ttataggcgg tgatgacggc ataacgccgc gatgttgggt
     5221 tatcaattcc gccgagataa acattgttca gcatcgccag atacgcggtg ccggtatcaa
     5281 tattgctggc aggatcaaac aagaaactgc ggctcggcgt gccggatttc ccctgcgagc
     5341 ggaacacatc tttcccggca gtatgttgta ccacctgcat taatcccagc gcatcggaac
     5401 ggctgaccgc atatgggtta aaggaagatt cggtctgcat aatcgccaga atcagcgact
     5461 catcaacgcc atatttccgt gacgcctggc ggaccatgcc gagatatttg tgcgcacgtt
     5521 tatcaaggtg gttcggcacc atgttaatgg taacgctgta gatgatacgc agcccgttgc
     5581 tgcggctctt cagacggttt ttcagcagat aatccgcgaa gttgctcgcg cgaccttccc
     5641 agcgaatcgg ctgcccggtg ttgtccacca cctgaccgta aaggaaaggt tctttcgaaa
     5701 tcgtaatatc atcaacgtcg gaatagagat cgaccgaact cggatcgtca cccatcagca
     5761 acgttttgat aattgcccgg cgcaaatgcg cggcaggttc tgtcccggcg atagtttcga
     5821 tagtaatcgt accgtcatcg aagttgatgt ggctgcgggt ctgatattga tcggtgtatt
     5881 tcacgtagtc cttaggaccg gcgatcacca cctctttgaa tccccagatg ttctcaatat
     5941 tgtgggcaaa ttgccccatc agaatatcaa aaccgttggt atctttgacc caggcttcgt
     6001 tataggtatc gccttttttg gtcgtcgaac aggagatgag caacggcgca atcaaagcca
     6061 gcgcgagata ttttttcatc attccgggtg cgtgttgtgt ttgtttttat gcaagttgcc
     6121 ggaggcgtgc tccggcactg tttttatttt ttatcttccg gcgtatagcc ctcgatatgc
     6181 acctctttac cctcgaacag gaagttgacc atctcctgct ccagcagctt gcggtgctcg
     6241 gcattcatca tgttgagttt cttttcatta atcagcatgg tttgtttgtg ctgccactgc
     6301 gcccaggctt ctttggagat ctcgttatag atgcgttttc ccagctcgcc ggggtacagc
     6361 tgaaaatcct gaccttctgc ttcacgttgc aggaaagtac aaaaaatcgt tctgctcata
     6421 aatcatcctc tttatcgact cacgcgctaa accggcgcgc cagtgcgtaa ctgctgtaac
     6481 aaacgctcca cgggagccgc caggccaact gacggcggtt gcgctaagtt ataccagagc
     6541 gcattgcctt catccatgca gccggtgaat gacgacacgg gaagccacat aggcacaata
     6601 tctaagtgga aatggctgaa ggtatgccga aacgcggtca gctgcgtcag gttatcggca
     6661 gcaatctgcc gttgcgccag ccactgccgc aaactttctt cgtcggcaaa ctgcgggaaa
     6721 cagtataaac cgccccacaa tccgctcggc ggacgctgcg ccagcaatac ttcatcttcg
     6781 tgctgtaata gcaaaaagta gccggtgcgc tccggcagcg tctgtttcgg ttttttgccc
     6841 ggataaagcg cccagctatt gttggcggcg gcaatacatc cgttttgtag cggacagagc
     6901 gaacatttcg gtttcgagcg cgtacaaatc atcgcaccca aatccatcat cgcctgatta
     6961 aaccgttcca cgccaaccgc gggcgtcacc tgctcgctca aactccataa tttattctcg
     7021 acctcttttt tccctggcca gccgcttaca gcatagcagc gcgccagcac ccgtttgacg
     7081 ttaccgtcga gaatcggaaa gtgcttaccc agagaaagcg agagaatcgc acctgcggtg
     7141 gaacgcccga cgcccggtaa cgccgcgact tcttcaaagg tttccgggaa tttaccgccg
     7201 tgtaaggtcg ccacttgttg tgccgcttta tgcaggttgc gcgcgcgggc gtaatagcca
     7261 agcccggtcc acaagtggag aacttcgtcg agcggcgcat tggcgagatc ggtcaccgtc
     7321 gggaagcgcg ccataaagcg ttcaaaatag gggataacgg tcgcaacctg agtttgttgc
     7381 aacatcactt ctgagagcca tactttgtag ggcgtcttgt caatttgcca gggcagcgtt
     7441 ttccgcccgt atttatcgta ccagtccaga acctgggctg aaaattgcga cgcttgcatg
     7501 gtcaccgaat tcactgttgt tgggggcaag attgcagcac agcgacggca gggtgtaaac
     7561 cggaactttc cgcagccacg gccttatcat gcttgcatcc ggcaactaac tttggataat
     7621 gcccgttttc agaacacttt cacaagcgac taaatcttta tgaaaaacga cgtcatttca
     7681 ccggaatttg atgaaaacgg ccgcccactg cgccgtatcc gtagttttgt gcgccgccag
     7741 gggcgactga ccaaaggcca ggaacatgcg ctggaaaact actggccggt gatgggcgtt
     7801 gagttcagcg aagatatgct ggatttcccc gcgctttttg gccgtgaagc gccggtgacg
     7861 cttgagattg gttttggcat gggggcgtcg ctggtggcaa tggctaaaga tcgccctgag
     7921 caggacttcc tcggcattga agtgcattca ccgggcgttg gtgcgtgcct ggcttctgcg
     7981 catgaagaag gtttaagcaa cctgcgcgtg atgtgtcacg atgcggttga agtgctgcat
     8041 aaaatgattc ctgacaattc attgcgcatg gtgcagctct ttttccctga cccgtggcac
     8101 aaagcgcgcc ataataaacg ccgtatcgta caggtgccgt ttgccgaact ggtaaaaagc
     8161 aaactgcagc tggggggcgt attccatatg gcgaccgact gggaacctta tgcggaacat
     8221 atgcttgaag tgatgtcttc tattgacggt tataaaaacc tgtcagagag caatgattac
     8281 gtaccgcgtc cggcatcacg tccggtgacg aaatttgaac aacgtggtca tcgtcttggt
     8341 cacggagtat gggacttaat gttcgagagg gtgaaataat ggcaaagaac cgtagccgtc
     8401 gtctgcgtaa aaaaatgcac atcgacgaat tccaggaatt aggattttcg gtggcatggc
     8461 gattcccgga aggtacatcg gaagaacaga ttgataaaac cgttgatgat tttattaacg
     8521 aggttatcga accgaacaaa ctggcctttg acggcagcgg ttatctggcc tgggaaggtc
     8581 tgatctgcat gcaggaaatc ggcaaatgca ccgaagaaca tcaggcgatt gtgcgtaagt
     8641 ggctggaaga gcgcaaactg gatgaggtac gcaccagcga acttttcgac gtttggtggg
     8701 actaagaaag catacgggcg atgacaaatg caaaactgcc tgatgcgcta cgcttatcag
     8761 gcctggaaag atgcacgatc gagtaggcgg gataaggtgt ttacgccgca tccggcatgg
     8821 aaaacgcgta ctttgttatc aatctggggc cagcaaatgc tggcctgatt ttttcttgag
     8881 ggaagactat gatgcgcaaa atgctgctgg cggcagcact ttcagtgacg gcaatgaccg
     8941 ctcacgccga ctaccagtgc agcgtcacgc cgcgtgacga tgtgattgtc agcccgcaaa
     9001 ccgtgcaggt gaagggcgaa aacggcaatc tggtgatcac gccagacggc aacgtgatgt
     9061 ataacggtaa gcaatattcc ctgaatgccg cccagcgcga gcaggcgaag gattatcagg
     9121 ctgaactacg cagcacgctg ccgtggattg atgaaggcgc gaaaagccgc gtcgaaaaag
     9181 cccgtattgc gctggataaa attatcgttc aggagatggg cgaaagcagc aaaatgcgca
     9241 gccgtctgac caaacttgat gcgcagctga aagagcagat gaaccgcatt atcgaaacgc
     9301 gcagcgatgg cctgacgttt cactataaag ccattgatca ggttcgcgcc gaaggccagc
     9361 aattagtgaa tcaggcaatg ggtggaattt tacaggacag cattaatgaa atgggcgcga
     9421 aagcggtgct gaaaagcggc ggtaacccat tacagaatgt gctgggaagc ctgggggggc
     9481 tgcaatcctc aatccaaacc gagtggaaaa agcaggaaaa agatttccag cagtttggca
     9541 aagatgtttg tagccgcgtt gtgactctgg aagatagccg caaagccctg gtcgggaatt
     9601 taaaataatc ctctatttta agacggcata atactttttt atgccgttta attcttcgtt
     9661 ttgttacctg cctctaactt tgtagatctc caaaatatat tcacgttgta aattgtttaa
     9721 cgtcaaattt cccatacaga gctaagggat aatgcgtagc gttcacgtaa ctggaggaat
     9781 gaaatggagt ttttcaaaaa gacggcactt gccgcactgg ttatgggttt tagtggtgca
     9841 gcattggcat tacccaatat caccatttta gcaaccggcg ggaccattgc cggtggtggt
     9901 gactccgcaa ccaaatctaa ctacacagcg ggtaaagttg gcgtagaaaa tctggttaat
     9961 gcggtgccgc aactaaaaga cattgcgaac gttaaaggcg agcaggtagt gaatatcggc
    10021 tcccaggaca tgaacgataa tgtctggctg acactggcga aaaaaattaa caccgactgc
    10081 gataaaaccg acggcttcgt cattacccac ggtaccgaca cgatggaaga aaccgcttac
    10141 ttcctcgacc tgacggtgaa atgcgacaaa ccggtggtga tggtcggcgc aatgcgcccg
    10201 tccacgtcca tgagcgcaga cggtccattc aacctgtata acgcggtagt ggccgcagct
    10261 gataaagcct ccgctaatcg tggcgtgctg gtggtgatga acgacaccgt actggacggt
    10321 cgcgatgtca ccaaaaccaa caccaccgac gtagcgacct tcaagtctgt taactacggt
    10381 cctctgggat acattcacaa cggtaagatt gactaccaac gtaccccggc acgtaagcac
    10441 accagcgata cgccattcga tgtctctaag ctgaatgagc tgccgaaagt cggcatcgtt
    10501 tataactacg ctaacgcatc cgatcttccg gctaaagcac tggtagatgc gggctatgat
    10561 ggcatcgtta gcgctggtgt gggtaatggt aacctgtata aatccgtgtt cgacaccctg
    10621 gcaaccgccg cgaaaaacgg cactgcagta gtgcgttctt cccgcgtacc gacgggtgct
    10681 accactcagg atgctgaagt ggatgatgcg aaatacggct tcgtcgcctc tggcacgctg
    10741 aacccgcaaa aagcgcgcgt cctgctgcag ctggctctga cgcaaaccaa agatccgcag
    10801 cagatccagc agatcttcaa tcagtactaa tcacctcgcc ccggtatcgt gccggggctt
    10861 tttcacttca gacttacgtc cattgccaat tttcattacc ctaatgataa tcaccggaat
    10921 aaattattcc gcgtgaggtt tttcgggtga aaaagcaatg gattgttggc acggcactgc
    10981 ttatgttgat gactggtaat gcctgggcag atggcgaacc gccaactgaa aatatcttaa
    11041 aagatcaatt caaaaagcag tatcacggca ttctcaagct tgatgctatc accttaaaaa
    11101 atcttgatgc taagggtaat caggccacct ggtcagcgga aggcgatgtc tcttccagtg
    11161 acgatctcta tacctgggtc ggtcagttgg cagattacga actgctcgaa cagacctgga
    11221 cgaaagataa accggtaaaa ttctcggcga tgttaaccag taaaggaacg cccgcgtctg
    11281 gctggtcggt gaacttttac tcttttcagg cggcagccag cgatcgtggg cgggtggtcg
    11341 acgatatcaa aacgaataat aaatatctga tcgtgaatag cgaagatttc aattatcgct
    11401 ttagtcagct tgaatctgcg ttgaataccc agaagaactc gattcctgcg ctggaaaagg
    11461 aagtgaaagc gcttgataag caaatggtgg cggcgcagaa agcagcggat gcatattggg
    11521 gaaaagatgc aaacggtaag caaatgaccc gcgaagaagc ctttaaaaaa attcaccaac
    11581 agcgtgatga gtttaataaa cagaacgata gcgaggcgtt cgcggttaaa tatgacaaag
    11641 aggtttatca accggcgatt gcggcatgtc ataaacagag tgaagagtgt tatgaagtgc
    11701 cgattcagca gaaacgagat ttcgatatca acgaacaacg gcgacaggct tttctgcaat
    11761 cacaaaaact cagccgtaaa ttgcaggatg actgggtaac gcttgaaaaa gggcaatatc
    11821 cgttaacgat gaaagtttca gaaataaaca gtaaaaaagt cgcgattttg atgaaaattg
    11881 acgatatcaa ccaagctaat gaacgctgga aaaaagatac cgaacaactg cggcgcaatg
    11941 gggtgattaa gtaagtttga tatagtgcct ttgccggata atataaaaag ttatccggca
    12001 aattattttt tacattagcc ctagtagctg tggaatgaac aaggatattt gcggaatgta
    12061 agtgatcatc atcaacgcta ctagcaatgc agcataaaat ggcagcaatg gtttgattaa
    12121 atgttgaatc ttcacacccg agatagaaca accaacaaat agcgcactac ccaccggcgg
    12181 tgttaacagc ccaatacaaa ggttggccac catcataata ccgaaatgta ctgggtccat
    12241 tcccagctct tgtgcaatcg gcaagaagat cggtgtaaag attagtacag ccggtgtcat
    12301 gtccataaag atgccgacaa ttaataagac gatattaatc agcagcaata tgatcagtgg
    12361 gttatcagaa atccccatca atgcgtcgct gatcatataa ggaatatcag cattagtcat
    12421 tgcccaggac atgcccacgg aaaaaccgat caacagtaat acaatcgaag tggtaacaac
    12481 cgactccaga atcagtttgg gtaaatggcg ccattttact tcccggtaaa ccagcaccga
    12541 gagaataaat gtgtagacaa cggcaattgc tgacgcttca gttgcggtaa aaatgccgcc
    12601 cagaatgccg cccatcacaa tgaacaccag aagcaaactt ggtaaggcat ccagaaacgc
    12661 tttacaggcc tgagcaaagg ttgctctttc tgaaagcggg tagccacggc gtttagcaat
    12721 aatgccgcag actatcatta ccgcaagacc cattaagatc cctggcaaat atcccgccat
    12781 aaatagcgat gctacagaaa cgccgccagc tgtcagggaa aacacaatta acacgtttga
    12841 gggcggtatc agtaatccag taatacaaga tgagacgttt acagcggtag aaaatgccgg
    12901 atcataacct tgtttggtct gaatgggatt cagtgtgcca ccaacggcag cagctgccgc
    12961 aacggctgaa ccagaaatgg aaccaaacat catattcgcc aggacgttca catgaccaag
    13021 tgaaccagga acccgaccaa ccattacctg ggcgaggtta atcaaacgta tcgcaatacc
    13081 gccgctgttc atcagcgttc cggcaaagat gaaaaagggg attgccaata gggcaaagtt
    13141 atccagtcca ttagccagtc gctgcgaaac agtgattgcc gcgatatccc agggaaacat
    13201 caaaagcatt gaagctacag tggcaatacc gattgagaac gaaatgggca caccaataaa
    13261 gaccaacaca aaaaaacagc caaacagtgt aatcgcgata taactatcca ttaactggcc
    13321 tcctgcgcat gttgtttcag ttgttgaatg ctttgattaa taaaacacaa ggcataaaac
    13381 atcatcacca atccacttaa tggcagaatg atatatacgt atcccatcgg tatttgcatc
    13441 gcggcagata cctgtgatgt tgccagcgtt ttatcaatta atttcaggcc accagtcaca
    13501 atgaccgaac cggcaaaacc taaaataaga acgttaatga ctatactaag aagtaattgc
    13561 ttgcgcttat tcagggcgag agcaaataaa tcgatagaga gatgtctctg cgcgccgacg
    13621 gtatatgcag cacctaataa ccctacccag atcatcaaaa atcgtgccag ctcatcggtt
    13681 aatgtacttg gctggttaag tacgtagcgg ctaaatactt gccagactac gcatacaacc
    13741 agagcaagca tgacggcgac agaaaacgcg gcaatgacgc ggtctaccgc aagtttcagg
    13801 cggttaaata tcattgtaag catcctggaa aatcagggcc agagactggc ccggaatatt
    13861 attgagcagc gttgtcgaac ttctctaaca aagcagcttg ttttggatct tttttgaagt
    13921 cgtcaaacag aggctgaact gcggcacgga atggtgcttt atccactttt acaatttcac
    13981 cccccatagc tttcgcctga gcacgtgtgt cagcgtcaat tttttcccac aatttttgtt
    14041 gataagcttc ggatttcttc gctgctgttt cgaggatttg ttgctgttct ggggttaatt
    14101 tattccatgt tttggtggag ataaccaaaa agtcaggaat tgaggcgtgt tcatcttcag
    14161 aaaaaacttt ggcaatttca atatgtcgag tctgcaccca ggaaggcacg ttattttctg
    14221 cgccatctac tacaccttgc tgcatcgcag tataaacttc gccaaatgaa attggagtgg
    14281 gtgaaccacc catcagttca atcattttga tggtggtcgg acttggctga acacgaattt
    14341 tcagcccttt aagatcttct ggcttagtaa tcggtttttt cgcatagaaa ctgcgtgtac
    14401 ccgctacata agctgataaa gcaaaaaagc ctttgtcttt tgttgagtcc ataatttctt
    14461 taccaacttc accaaaaaca actttgttga agtgagcctg atctttaaat aagaacggca
    14521 aattatagat agcataaata ttatcaaaag actccagatc actggcggaa cctttggtca
    14581 tatccagagc gccattttgc aacaactcca tagtttctcg cgcattaccc atctggctgc
    14641 tgggataaat acggatcacc atattacctt ttgacagctg ttttacctct ttcgccagct
    14701 cttcaaaact ctgatgtaca acatgacttc tttccaggtt gtgtgctaac tttaacgtca
    14761 ctttttctgc ggcgtttacg ccagtgctaa ccagagccag caaggatgcg cagactaatg
    14821 agcgggtaaa tgacctcatt ttcataaaaa aactccctgt tgaaggatgg aaattggttg
    14881 atatctaatt gtgcttatca gtgctttgtg cgacaaattg gttaacctgt ctgacaactt
    14941 gtctatgttt atcacaactt gaaataaagt c
//
LOCUS       DAZFWL020000116        14826 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFWL020000116 DAZFWL020000000
VERSION     DAZFWL020000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.116"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..152)
                     /locus_tag="ACHKNP_RS18645"
                     /old_locus_tag="pgaptmp_003729"
     CDS             complement(<1..152)
                     /locus_tag="ACHKNP_RS18645"
                     /old_locus_tag="pgaptmp_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705821.1"
                     /translation="MKIEYTPERGRGFVRPGETEKQQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCV"
     gene            complement(210..398)
                     /locus_tag="ACHKNP_RS18650"
                     /old_locus_tag="pgaptmp_003730"
     CDS             complement(210..398)
                     /locus_tag="ACHKNP_RS18650"
                     /old_locus_tag="pgaptmp_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4705822.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(773..2002)
                     /locus_tag="ACHKNP_RS18655"
                     /old_locus_tag="pgaptmp_003731"
     CDS             complement(773..2002)
                     /locus_tag="ACHKNP_RS18655"
                     /old_locus_tag="pgaptmp_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4705823.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGSYPDLSLKAAREKAAQCRTWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLEYYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNVLDMWMERLGI
                     LAGTHENVTTLPVARRK"
     gene            1990..2172
                     /locus_tag="ACHKNP_RS18660"
                     /old_locus_tag="pgaptmp_003732"
     CDS             1990..2172
                     /locus_tag="ACHKNP_RS18660"
                     /old_locus_tag="pgaptmp_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705824.1"
                     /translation="MLCSRFTPSLRKSDYTSDYTDRCTKGYNVKQAGTRKITNLLIIK
                     NIMHSLKHYETAKNTK"
     gene            2179..3084
                     /gene="tqsA"
                     /locus_tag="ACHKNP_RS18665"
                     /old_locus_tag="pgaptmp_003733"
                     /pseudo
     CDS             2179..3084
                     /gene="tqsA"
                     /locus_tag="ACHKNP_RS18665"
                     /old_locus_tag="pgaptmp_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(3109..4497)
                     /gene="pntB"
                     /locus_tag="ACHKNP_RS18670"
                     /old_locus_tag="pgaptmp_003734"
     CDS             complement(3109..4497)
                     /gene="pntB"
                     /locus_tag="ACHKNP_RS18670"
                     /old_locus_tag="pgaptmp_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HGU4705825.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(4508..6040)
                     /gene="pntA"
                     /locus_tag="ACHKNP_RS18675"
                     /old_locus_tag="pgaptmp_003735"
     CDS             complement(4508..6040)
                     /gene="pntA"
                     /locus_tag="ACHKNP_RS18675"
                     /old_locus_tag="pgaptmp_003735"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HGU4705826.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            6564..7508
                     /gene="ydgH"
                     /locus_tag="ACHKNP_RS18680"
                     /old_locus_tag="pgaptmp_003736"
     CDS             6564..7508
                     /gene="ydgH"
                     /locus_tag="ACHKNP_RS18680"
                     /old_locus_tag="pgaptmp_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HGU4705827.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            7694..9076
                     /gene="ydgI"
                     /locus_tag="ACHKNP_RS18685"
                     /old_locus_tag="pgaptmp_003737"
     CDS             7694..9076
                     /gene="ydgI"
                     /locus_tag="ACHKNP_RS18685"
                     /old_locus_tag="pgaptmp_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4705828.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9113..9835
                     /gene="folM"
                     /locus_tag="ACHKNP_RS18690"
                     /old_locus_tag="pgaptmp_003738"
     CDS             9113..9835
                     /gene="folM"
                     /locus_tag="ACHKNP_RS18690"
                     /old_locus_tag="pgaptmp_003738"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HGU4705829.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(9832..10167)
                     /gene="ydgC"
                     /locus_tag="ACHKNP_RS18695"
                     /old_locus_tag="pgaptmp_003739"
     CDS             complement(9832..10167)
                     /gene="ydgC"
                     /locus_tag="ACHKNP_RS18695"
                     /old_locus_tag="pgaptmp_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HGU4705830.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            10296..11015
                     /gene="rstA"
                     /locus_tag="ACHKNP_RS18700"
                     /old_locus_tag="pgaptmp_003740"
     CDS             10296..11015
                     /gene="rstA"
                     /locus_tag="ACHKNP_RS18700"
                     /old_locus_tag="pgaptmp_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HGU4705831.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11019..12320
                     /gene="rstB"
                     /locus_tag="ACHKNP_RS18705"
                     /old_locus_tag="pgaptmp_003741"
     CDS             11019..12320
                     /gene="rstB"
                     /locus_tag="ACHKNP_RS18705"
                     /old_locus_tag="pgaptmp_003741"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HGU4705832.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            12396..13325
                     /gene="tus"
                     /locus_tag="ACHKNP_RS18710"
                     /old_locus_tag="pgaptmp_003742"
     CDS             12396..13325
                     /gene="tus"
                     /locus_tag="ACHKNP_RS18710"
                     /old_locus_tag="pgaptmp_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HGU4705833.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13322..14725)
                     /gene="fumC"
                     /locus_tag="ACHKNP_RS18715"
                     /old_locus_tag="pgaptmp_003743"
     CDS             complement(13322..14725)
                     /gene="fumC"
                     /locus_tag="ACHKNP_RS18715"
                     /old_locus_tag="pgaptmp_003743"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HGU4705834.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAVSLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         3545 a         3865 c         3583 g         3833 t
ORIGIN      
        1 acacaaacgg cgcagcgggt tatctgttca gaaaggcggc tccatttcgg ggccgctttt
       61 tttatacctg aaaaacccca attttgttgt ttttcagttt caccagggcg aacgaatccc
      121 cgcccacgtt ctggcgtata ttcaatcttc atggttatgg ctctgtgttc agatgattga
      181 tgtgtggcgg ctgcgtgccg ccagcgtgat taatgaactg ccttgcagct atccttccag
      241 gccagaacct cggataaaga ccagccaacg gaacgaccgc caagtttacg acgtgatggg
      301 aattgtccgg ccttttccag gcggtagcgg catgagcggc taaggcctgt tagcttttcg
      361 cattcttttt cacgtataaa ccgatcagtg cttaacacta ttgccccctt tcgtttctta
      421 aagagttatt tcgtgttcta ttgcgttggg atgtgtctga ttgtgtcagg atgattcaga
      481 gttggcaaat gttgaggccg tatggtttac agaaagagga ataatcagga taaaatcatt
      541 taaattcatg ttaatacaaa ggcataaaat attgtttcat gccttttttc tcgctattta
      601 aagagtgatt cgctagtgta taaaaaacca gtaacacatt aaaaatcagc tacttataaa
      661 tctgtacact ttttcgcctc ttgttcgtag ttgttccgca ttgttgctca ttgttgcacg
      721 ttgtatctgt tcgcatatcc agtatgcgca tactgaaaaa atacgaaaaa aattattttc
      781 ttctggctac tggtagcgtg gttacgtttt catgtgttcc cgccagtatc cctaaccgct
      841 ccatccacat atccagcaca ttgcgtttag catccagata acgggaatga ttataaactc
      901 gctgcattcc tggcatctgg tggcctgtaa gctgctccac gacatgcgga tcaacgccta
      961 aatcgttcag catggttgta aaggtgcgcc ggatgtcatg cagtgaccag tgaggatgat
     1021 taagcctcct gtgcgctaat cttccatact gcgatacgct ggcctcctgt ttcacttccc
     1081 ccagcaataa gcccgtgtgc ctgttctgct ccaccagctg cgtgacgaac ggcaggatcg
     1141 cttccggtat gggccggaat attgcgacct tcgttttgct gtgctccttc ggaacggtcc
     1201 atagcatttc cgtaaaatcc cactccccga tctccgatag cctcagttct accgtcctgg
     1261 ctccgaagac aatcaggagg cggattaacg cgacgtagta aggggaaaat atttttttgt
     1321 ccagtgcctg caataattcg ccaagttctt tgttacttaa gacacgttcg cttatatccg
     1381 gttttttccc aacgtccgcc acgttcagat cgtccagaac gttgctgatt gcatagcgcc
     1441 gcctacggca gaacttaagc gcctgtttgc acgcctgtag cacgaatccg gcagtaacag
     1501 gcgttcgctt tgccacctgg tcaaaacagg ccagccagtg ccgtagctcg catttatcca
     1561 gcggcatagc accaatctgc tgtattacgt gattattaag tcgccttttc agggcgatat
     1621 aatccacgcg gttttccttt acgtaatact caagccagta ggtgagcgca tcgccaaccg
     1681 ttacgggctt taacgcttcc tgtacggtgt aattcatctc atgacgtgga tttttcccct
     1741 cagccagcca tgtgcgacac tgtgcggctt tttccctggc tgctttcagg ctcagatcag
     1801 gataacttcc cagcttaatg cgttcgggtc gtgtctccct tcccgttccg gccctgtatg
     1861 tgaaatacca ggtcaatttc cctgatgtta aatatttcac gctcaggttt ccgccatcac
     1921 tataaaacgt gtttttctcc gctggcttac catgaagttt ccttagcaag gtatcgctca
     1981 gtttgttcat tgctctgctc acgttttacc ccctctttac gaaaaagtga ctacaccagt
     2041 gactacaccg atcgttgcac aaagggctac aacgtgaaac aagctggaac aaggaaaatc
     2101 acaaatctgt tgataatcaa aaacataatg cacagcctga aacattatga aacagcaaaa
     2161 aacactaaat gataaaatgc tgttattctt aacccgctgg tgcaacacat ggtccgctgg
     2221 cgagtgccgc gtgtactggc ggtgtcgatt ttgatgacca tcatcgtgat ggcgatggtg
     2281 ttgctattag cttatctggg ttccgcgctc aacgagttga cgcggacgtt accgcaatat
     2341 cgcaactcta ttatgacgcc gctgcaagct cttgagccgt tgttgcaacg cgtagggatt
     2401 gacgtctcag ttgaccagct ggcgcattac attgatccga acgcggcgat gacgttgctc
     2461 accaacttat tgacgcagtt atctaatgcc atgtcatcaa tatttttatt gctgctgacg
     2521 gtgctgttta tgctgctcga agtgccacaa ttgcccggaa aatttcagca aatgatggcg
     2581 cgtccggttg aagggatggc ggcgattcaa cgtgcaattg acagtgtttc tcattatctg
     2641 gtgctgaaaa cagccatcag catcatcacc ggcctggtcg cctgggcgat gctcgccgca
     2701 ctcgatgttc gcttcgcttt tgtctgggga ttgctggcct ttgcgcttaa ttacatccct
     2761 aatattggtt cagttctcgc ggcaatcccc cctatcgctc aggtactggt gtttaatggc
     2821 ttctacgaag cgttgctggt gctggcggga tatctgctga ttaatctggt cttcggcaat
     2881 attctggagc cgcgcatcat gggacgtggg ctggggcttt ccacattggt ggtatttttg
     2941 tcgttgattt tttggggatg gttgttagga ccggtgggta tgctgctttc cgtgccgtta
     3001 acaattattg tcaaaattgc gcttgaacaa acagcgggag gtcaaagcat cgccgttctg
     3061 ttaagcgatc tcaataaaga gtgacggcct ccgcagaggc cgtcagggtt acagagcttt
     3121 caggattgca tccacgctgg ctttggcgtc accaaacagc atgtgggtgt tttccttgaa
     3181 gaacagcggg ttttgcacac cagcatagcc agtgttcatc gaacgtttaa agacaatcac
     3241 gttctgcgct ttccacactt ccagcacagg cataccagca ataggactct tcggatcatc
     3301 ctgcgccgcc gggttaaccg tatcgttagc accaatcacc agtacggtat cggtatcagc
     3361 aaagtcatcg ttgatctcgt ccatttccag cacgatgtca tacggtactt ttgcttcagc
     3421 cagcaatacg ttcatatgtc caggcaaacg ccccgcaacc gggtggatac cgaaacgcac
     3481 gttgatacca cgagcgcgca gtttctcggt aatttcagcg acaggatatt gcgcctgcgc
     3541 gactgccatg ccgtaccccg gagtaatgat cactgaatgg gagtttttca gcagttccgc
     3601 tgtctcttct gcggtgattt cgcggtgctc gcccacttcc tgatcatcgc cagtagaaga
     3661 gccgtcggtg ccgaaaccac ccgcaataac gctgataaag gaacggttca tcgccttaca
     3721 cataatgtaa gaaaggatag cgcccgaaga accgaccagc gcaccggtca caatcagcag
     3781 gtcgttgctg agcataaagc ccgcagccgc agccgcccag ccggagtacg agttcagcat
     3841 cgacaccacc actggcatat ctgcaccacc gatggaggcg actaaatgcc agccgaatac
     3901 cagcgcaatg gcggtcatta tcagcaatgc cagcacttgc aggccgacgc tatccgtgcg
     3961 aacaaataca atcagcagca ggaaggaaac gaccagagcc gccaggttca ttttgtgacg
     4021 gtttggcagc atcaacggtt tagacgaaat cttgccacac agtttgccga acgccaccac
     4081 cgaacccgtg aacgttaccg ccccgatgaa gataccgagg aacacttccg tcaggtgaat
     4141 attgaccaga atcggtgcca ttcccgcgtc atgatgcaga taactgttaa agccaaccag
     4201 cactgccgcc agacccacga agctatgcag gatcgccacc agttctggca tttcggtcat
     4261 ttcaactttc ttcgccagac ggataccaat tgccccacca atgaccatcg ccagcaagat
     4321 ccagccaaca ttacccgtat ccggtccaaa aatggttgcg attaacgcaa tcgccatccc
     4381 ggcgataccg aagttgttac cctggcgaga cgtttcatgt ttcgaaagac cggccagact
     4441 gaagataaac aggatcgcgg caacaatgta tgcagctgta actaatcctc cagacatatg
     4501 ttacccctta atttttgcgg aacattttca gcatgcgctg agtcacggtg aagccaccga
     4561 aaatattaat gctggctata agcaccgcga taaaactaag gaagctaacc cagccgccct
     4621 ggccaatctg caacagtgct ccgacaacaa taatccctga aatcgcgttg gtgaccgaca
     4681 tcaacggtgt atgcagcgca tgcgatacat tccacaccac gtaataaccg acaacgcagg
     4741 ccagcgcgaa aacggtgaag tgcccaagaa actctttcgg cgcaacgctt gccagccaac
     4801 cgaaaaggat gattgccagc gccatcaatg cgtatttacg ccacggtgag caggcacatt
     4861 tttcctcagt tttcacttcc ggtgccgctt tttgtgccgc ctgcggctga gctgatacct
     4921 gaatcggcgg tgccggccag gtaatttcgc ccgcacggat cacggtcacg ccgcgaatca
     4981 ccacatcatc aaaatcaaca gtgatattgc cgtctttctc tttgcacaac agtttcagca
     5041 gattaacgag gttagtaccg taaagctgtg aggattgcgt cggcagacgg cccggaagat
     5101 cggtataacc aatcactttg acaccatttt ccgtagtgaa gatttcaccc ggcacggtgt
     5161 attcacagtt gccaccgttt tgggctgcca ggtcgacaat cacactgccc gccttcatgg
     5221 agtcaaccat ttcacgggta attagcttcg gcgctggttt gcctggaata agcgcggtgg
     5281 tgacaatgat atcgacctct tttgcctggg cggcaaagag ttccatttcc gctttgatga
     5341 acgcgtccga catcactttg gcatagccat cgccgctgcc cgcttcctct ttaaaatcca
     5401 gctcgaggaa ttccgcgccc atactttgaa cttgttcttt cacttccggg cgggtgtcga
     5461 atgcacgcac aatcgcgccg agactgtttg ctgcgccaat ggcggccaga cctgcaacac
     5521 ccgcaccaat caccatcact tttgccggtg gcactttccc ggccgcagta atttgcccgg
     5581 taaagaagcg cccaaattca tgtgccgctt caacaatggc gcgataaccg gcgatgttcg
     5641 ccatcgagct tagtgcgtcc agcgattgtg cgcgtgagat acgcggcaca gaatccatcg
     5701 ccatcacggt cacgttacgt tccgcaagtt tttgcattaa ttccggattc tgcgcaggcc
     5761 agataaaact caccagcgtt gtcccaggat tcagtaacgc aatttcatca tctaacggcg
     5821 cattgacctt cagaatgatc tctgactgcc agacgctatt cccttctaca atttcagcgc
     5881 ccgcttgcac aaacgcttta tcgtcaaaac ttgccagttg acccgcgccg ctctctaccg
     5941 cgacggtaaa acccagtttc agcagctgtt ccactgtttt tggcgttgct gcaacacggg
     6001 tttcattggt taaccgttct cttggtatgc caattcgcat gatattccct tccatcggtt
     6061 ttattgatga tggtttgcct gtgtcaggag ccacacaagc cgctcatgta cgagctaaat
     6121 gttactccgt taaaataaat tagtaacaaa cgccctataa cgtactgaaa attatgcctg
     6181 tgatctagcg ccaaaaatca gtatttcggc gtgaactcgc aaaatattaa cgattcagcc
     6241 gtgatagtgg gataaacacc ttagaacgcc ggataaagac tgataattgt cttcgacggt
     6301 cgggtaaaac gagacatcgc cccggcacga atcactactt aacattaaat taacttatac
     6361 aattcagttg cttcagtagt aatgatgctg atacggctgt tttttaagca tagacggtca
     6421 tttgagcagg attaaaattg gcttaaggaa tgtgatatga aaaatgacgc agacagttac
     6481 accgtttaaa tgcaataatc agccacgttt ctcgttaata acaataccag tacctggttt
     6541 gcgcaaggcg aaggattatt tttatgaagc ttaagaacac cctcctggcg tcggcactgc
     6601 tttctgctac ggcattctcc gttaacgcag caacagaact gacaccggag caagcggcag
     6661 cggttaaacc ttttgaccgt gtagtggtta ccggtcgttt taatgctatt ggcgaagcgg
     6721 tgaaagccgt ttctcgtcgc gcagataaag aaggtgccgc ctctttttat gttgtcgaca
     6781 cttctgattt tggtaacagc ggtaactggc gtgtggtcgc tgacctctat aaagccgatg
     6841 ctgaaaaagc agaagaaaca agtaatcgcg taattaacgg tgttgtcgaa ctgccgaaag
     6901 atcaggctgt tctgattgaa ccgtttgaca cggtcaccgt ccagggcttc tatcgtagcc
     6961 agccagaagt caatgatgcc atcaccaaag cagcaaaagc gaaaggtgcc tactctttct
     7021 acattgttcg tcaaatcgat gccaaccagg gcggcaacca gcgtattact gcattcatct
     7081 ataaaaaaga tgctaagaaa cgtatcgtcc agagcccgga tgtgatcccg gcagattccg
     7141 aagcaggacg tgcagctctg gctgccggtg gcgaagccgc gaagaaagtt gagatcccag
     7201 gtgttgcgac taccgcatca ccaagttctg aagtcggtcg tttctttgaa acccagtcat
     7261 caaaaggcgg acgttacact gtcacgcttc ctgacggcac taaagtcgaa gaactgaaca
     7321 aagcgacagc agcgatgatg gtcccgttcg acagcatcaa attctctggc aactacggca
     7381 acatgaccga agtctcctat caggttgcga aacgtgccgc gaagaaaggt gctaagtatt
     7441 accacatcac ccgccagtgg caggaacgtg gtaataacct gaccgtcagc gcagatctgt
     7501 ataaataact attcagttac tcctataagg cggcttgatg ccgcctttcg catttttagt
     7561 cacaaatttc cccgcgacca ttgcatgcag acacatctct tcgtaaaatc ccgcgcttca
     7621 gtggcaaact ccatttttat gcgttttgcc ctaatattta ttcttcttat cacgttttaa
     7681 tcactggata tcgatggaaa agaaactggg actgagcgca ctcaccgcgc tggtattaag
     7741 ctcaatgctg ggcgcgggtg ttttcagtct gccgcaaaat atggcggcag ttgccagccc
     7801 ggcagcactg ctcatcggct ggggtattac tggcgctggc attttattgc tggcctttgc
     7861 catgctgatc ctcacgcgca ttcgtcccga acttgacggc ggtatcttta cctatgcccg
     7921 cgaagggttt ggcgagctaa tcggcttttg ttccgcatgg ggatactggc tgtgcgcagt
     7981 catcgccaac gtctcctatc tggtgatcgt tttttccgcg ttaagctttt ttacggacac
     8041 gccggaattg cgcctgttcg gcgatggtaa tacctggcaa tcgatagtcg gtgcatcggc
     8101 tttattgtgg attgttcact ttttgatcct gcgcggggtg caaaccgctg ccagcattaa
     8161 cctggtggcg acactggcaa aattgttgcc gctgggtctg tttgttgtgc tggcgatgat
     8221 gatgttcaaa ctggatacct tcaagctcga cttcaccgga cttgcacttg gcgtacccgt
     8281 gtgggaacag gtgaaaaaca ccatgctgat caccctgtgg gttttcattg gtgtggaagg
     8341 tgccgtggtt gtttctgcgc gtgcgcgtaa taaacgtgat gttggcaaag cgacactgct
     8401 ggcggttctc tccgctctgg gcgtttactt gctggtaacg ctgctttcgc tgggcgtggt
     8461 ggcacgtcct gaactggctg aaattcgtaa cccgtcaatg gccggtctga tggtggaaat
     8521 gatgggacca tggggcgaaa tcatcatcgc tgccggtttg atcgtttccg tttgcggcgc
     8581 gtacttaagc tggaccatca tggcggcaga agttccgttc ctggctgcca ctcataaagc
     8641 attcccacgc atttttgcgc gccagaacgc acaagctgcg ccatccgcct cgctgtggtt
     8701 gaccaacatc tgcgtgcaaa tttgtctggt actcatctgg ctgaccggtt ccgattacaa
     8761 cacgttgctg accatcgcct cagaaatgat tctggtgccc tatttcctgg tcggcgcgtt
     8821 cttgctgaaa atcgcaaccc gtccactgca taaagcggta ggtgtcggtg cctgcattta
     8881 tggcttatgg ttattgtatg cctctggtcc aatgcacttg ctgttatccg ttgttctgta
     8941 tgcgccgggg ctgctggttt tcctgtatgc gcgcaaaacg catacacatg ataatgtact
     9001 gaaccgtcag gaaatggtgc tgattggcat gctgttaatt gcctcagttc cggcgacctg
     9061 gatgctggtg ggataacgtt cataccatcg ttggatagca aaggagataa cgatgggtaa
     9121 agcccagccc ttgccaatat taattactgg cggaggtcgt cgcatcggcc tcgccctcgc
     9181 ctggcatttc attaatcaaa agcaaccggt gattgtcagc tatcggacgc actatccagc
     9241 cattgatgga ctgattaagg caggtgcgca gtgtattcag gctgattttt cgaccaacga
     9301 cggtgtgatg gcgtttgccg atgaagtact aaaaagcacc catggtctgc gtgctatttt
     9361 gcataacgcc agtgcgtgga tggcggaaaa accaggtaca ccactggccg acgtactggc
     9421 ttgcatgatg cagatccacg ttaatacccc atacctgctc aaccatgcgc tggaaagatt
     9481 actgcgtggg cacggacacg ccgccagcga tatcattcac tttaccgatt atgtggtgga
     9541 gcgcggtagc gacaaacatg ttgcgtatgc tgcaagcaaa gcggcactgg ataatatgac
     9601 ccgctcgttt gcccgcaagc tggcaccgga agtgaaagtg aattctattg cgccatcgct
     9661 gatcctgttt aatgaacatg atgatgccga atatcgacaa caggcgctga ataaatcact
     9721 gatgaaaacc gcgcctggcg agaaagaagt gatcgacctg gtcgattact tacttaccag
     9781 ctgctttgtc accggacgca gtttcccact tgatggcggt cgtcatctgc gttaatgcag
     9841 ctttatccag catataatca acacccatgc gcttatcccc caacacgcaa cagaaccaac
     9901 aaaagcggcg ggcaagcgca tcattccggt gaaataccac agcgacacca ggtaaacaaa
     9961 atagggaata atcgaccaca tactaaaaat gatggttgcg cgtaaggctt caatgccgcg
    10021 ttcgctggca acaatataat gcgcgataag cgcaaaggtc gggaaaagtg gaatcagccc
    10081 ggcgatataa taatttttcg tttttgctaa aacaccaatc aacagcacta ccagcgcacc
    10141 gagcgcggct ttgattacca gccccatctt tttaccttaa cacttccata acaagtcatc
    10201 agtagaatac ctgatgaaaa cttgtttaga aacgattgat agtaagtaaa aacagcgcgg
    10261 tgtattgtga cgtttttata tctaccgtga atgttatgaa cactatcgta tttgtggaag
    10321 atgatgcgga agtcggttca ctgattgccg cgtacctggc aaaacatgat atgcaggtta
    10381 ccgtagaacc gcgcggcgac caggccgaag aaaccatttt gcgagaaagt ccggatttgg
    10441 tgttactcga catcatgcta ccaggcaagg acggcatgac catttgtcgt gatttacgcg
    10501 caaagtggtc tggaccgatt gttcttctaa cctctctcga tagcgatatg aaccacatcc
    10561 tggcactgga aatgggtgcc tgcgactata ttctcaaaac gacgccccct gctgttttgc
    10621 tagcgcgttt acgtttgcat ttgcgtcaga atgagcaagc cacactgacc aaaggtcttc
    10681 aggaaacgtc tctgactccc tacaaagccc tgcatttcgg cacgttgacc atcgatccca
    10741 tcaaccgcgt agtcaccctg gctaacactg aaatctcgct ctcgacagct gatttcgaat
    10801 tattgtggga attagctacc catgccgggc aaatcatgga ccgcgatgca ttgctgaaaa
    10861 atttacgcgg cgtcagttat gacggactgg atcgtagcgt ggacgtggct atttcgcggt
    10921 taagaaaaaa actgctcgat aacgccgcag aaccttatcg cattaaaact gtgcgtaaca
    10981 aaggctatct ttttgcgcct catgcatggg aataagcgat gaaaaaactg tttatccagt
    11041 tttacctgtt attgtttgtc tgcttccttg tgatgtctct gctggttggg ttggtgtaca
    11101 aatttaccgc cgaacgcgcg ggcaaacagt cgctggatga tttgatgaac agttcgctgt
    11161 atctgatgcg cagcgaattg cgtgagatcc ccccacacga ctggggtaag acgctgaaag
    11221 agatggattt aaatctctct ttcgatctgc gtgtcgagcc actgagtaaa taccatcttg
    11281 atgatatttc catgcaccga ctgcgtggcg gcgaaattgt cgccctggac gatcagtaca
    11341 cgtttttgca gcgtatcccg cgcagccact acgtgctggc agttggtcct gttccttatc
    11401 tttattatct ccatcagatg cgattgctgg atatcgccct gatcgctttt attgctattt
    11461 ccctcgcctt tccggtgttt atctggatgc gtccgcactg gcaggatatg ttaaaactgg
    11521 aagcagcggc gcaacgattt ggcgatgggc atctcaatga gcgtatccac tttgatgagg
    11581 gttcgagctt tgaacgactt ggtatcgcat ttaaccagat ggcggacaat atcaacgcct
    11641 tgattgccag caaaaaacag cttattgacg gtatcgctca cgaactgcga acaccgttag
    11701 tgcgcctgcg ttatcgactg gagatgagcg ataacctgag cgccgccgaa tcccaggcgt
    11761 tgaatcgtga tatcagtcaa cttgaagctt taattgaaga gctgctgact tatgcccgac
    11821 tcgatcgccc acaaaacgag cttcatctta gcgaaccaga cctgccgttg tggctgtcaa
    11881 cgcatctggc agatattcag gcagtaacgc ccgataaaac ggtacggata aaaacgctcg
    11941 tgcaaggcca ttatgcggcg ttggatatgc gcttaatgga gcgcgtgctg gataatttgc
    12001 tcaataacgc cctgcggtac tgccattcaa cggttgaaac cagcctgcta ctgtcgggga
    12061 atagagcgac attaattgtt gaggatgatg gcccagggat tgccccagaa aaccgcgaac
    12121 atatctttga accttttgtt cgcctcgatc ccagccggga tcgctcaacc ggcggctgcg
    12181 ggctggggct ggcaattgtc cactctatag cactggcaat gggcggtacg gttaattgtg
    12241 acaccagcga actgggtggt gctcgcttct cgtttagctg gccgttatgg cataacatcc
    12301 cgcaatttac ctctgcctga cactacgcgc acgatggtca agtcaccacg actgtgctat
    12361 aaaataagta tgttgtaact aaagtggtta atattatggc gcgttacgat ctcgtagacc
    12421 gactcaacac tacctttcgc cagatggaac aagagctggc tacatttgcc gctcatcttg
    12481 agcaacacaa gctattggtt gcccgcgtgt tctctttgcc ggaggtaaaa aaagaggatg
    12541 agcataatcc gcttaatcgt attgaggtaa aacaacatct cggcaacgac gcgcagtcgc
    12601 tggcgttgcg tcatttccgc catttattta ttcaacaaca gtccgaaaat cgcagcagca
    12661 aggccgctgt ccgtctgcct ggcgtgttgt gttaccaggt cgataacctt tcgcaagcag
    12721 cgttggtcag tcatattcag cacatcaata aactcaagac cacgttcgag catatcgtca
    12781 cggttgaatc agaactcccc accgcggcac gttttgaatg ggtgcatcgt catttgccgg
    12841 ggctgatcac ccttaatgct taccgcacgc tcaccgttct gcacgacccc gccactttac
    12901 gctttggttg ggctaataaa catatcatta agaatttgca tcgtgatgaa gtcctggcac
    12961 agctggaaaa aagcctgaaa tcaccacgca gtgtcgcacc gtggacgcgc gaggagtggc
    13021 aaagaaaact ggagcgagag tatcaggata tcgctgccct gccacagaac gcgaagttaa
    13081 aaatcaaacg tccggtgaag gtgcagccga ttgcccgcgt ctggtacaaa ggagatcaaa
    13141 aacaagtcca acacgcctgc cctacaccac tgattgcact gattaatcgg gataatggcg
    13201 cgggcgtgcc ggacgttggt gagttgttaa attacgatgc cgacaatgtg cagcaccgtt
    13261 ataaaccaca ggcgcagccg cttcgtttga tcattccacg gctgcacctg tatgttgcag
    13321 attaacgccc ggctttcata ctgccgacca tctgttctgg ccgtacccag ctgtcaaact
    13381 cggcttcgct aagatacccc agcgcaaggg ccgcagcttt taaggtcagc ccttctttat
    13441 gcgctttttt ggcgatctcc gcggctttgt cataaccaat gtgggtgtta agcgcagtca
    13501 ccagcatcag cgattcattg agtaattgat tgattcgctc acgattcggt tcaataccca
    13561 ctgcgcagtg tttgttaaaa ctttccatgc catctgccag caagcgcacc gattgcagga
    13621 aattgtggat caccattgga cggaagacgt tcagttcaaa gttaccggaa gcgcccccca
    13681 tgttgatcgc cacgtcgttc cccatcacct gacagcagag catggttaat gcctcacact
    13741 gtgttggatt cactttcccc ggcatgattg agctgcccgg ctcattttcc gggattgaga
    13801 tttcaccaat tccgcagcgc gggccagagg ccagccagcg gacatcattg gcgattttca
    13861 tcagtgacac agccaaccct ttcaacgcgc cgtgcgcctg aaccagggca tcacaggtcg
    13921 ccagcgcttc aaatttgttc ggcgcggtaa caaacggtgc acaggtaatg actgccagtt
    13981 catctgctac gcgacgcgca tactccggat gggtatttag tccagtaccc accgctgtac
    14041 cgccaagagc cagttccgct acgtgaggca ggctgtattc gatatgtttg agattatgct
    14101 cgagcatcgc tacccagccg gaaatctcct gccctagtgt tagcggcgtg gcgtcctgca
    14161 agtgggttcg accgattttg acgatatcgg caaatgcacg cgatttttca ctcagtgtct
    14221 gtgtcagggt tttaagctgc ggaatgagtt gcttgcgcag cgccagcagc gccgcaacgt
    14281 gcatcgccgt cggaaagaca tcgttggaac tttggctttt gttcacgtcg tcgttagggt
    14341 gaactttacg ttccatcccg cgcacgccgc cgagtaattc actggcccgg ttagccagca
    14401 cttcgttcat gttcatgtta ctttgcgtgc cggagccggt ctgccagata gccagcggga
    14461 attcgtcgtc atgctgtcct gccagtactt catccgccgc ctgccgaatg gcgctcgctt
    14521 tctcttcaga caacaagcct aaatcttcat taacttttgc cgctgcgcgc ttggttagcg
    14581 ccagcgcatg aatcagtgag gtgggcattt tctccgtcga aatgcggaaa tgctccagcg
    14641 agcgttgagt ttgtgcgccc cacagcttat ctgccgggac atcaatcgcc cccatcgaat
    14701 ctttttcgct gcgtactgta ttcatggcct gctcctcacc tgtttaattt tttctttctg
    14761 ttttgctttc gttaagcaac ttttagctca cttattattt actatttgat aacaaatgtt
    14821 tggtct
//
LOCUS       DAZFWL020000117        14806 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFWL020000117 DAZFWL020000000
VERSION     DAZFWL020000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.117"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..77)
                     /gene="hokE"
                     /locus_tag="ACHKNP_RS18720"
                     /old_locus_tag="pgaptmp_003744"
     CDS             complement(<1..77)
                     /gene="hokE"
                     /locus_tag="ACHKNP_RS18720"
                     /old_locus_tag="pgaptmp_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU4705835.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVG"
     gene            46..315
                     /locus_tag="ACHKNP_RS18725"
                     /old_locus_tag="pgaptmp_003745"
     CDS             46..315
                     /locus_tag="ACHKNP_RS18725"
                     /old_locus_tag="pgaptmp_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705836.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            519..1637
                     /gene="ybdK"
                     /locus_tag="ACHKNP_RS18730"
                     /old_locus_tag="pgaptmp_003746"
     CDS             519..1637
                     /gene="ybdK"
                     /locus_tag="ACHKNP_RS18730"
                     /old_locus_tag="pgaptmp_003746"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HGU4705837.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1703..1951
                     /gene="ybdJ"
                     /locus_tag="ACHKNP_RS18735"
                     /old_locus_tag="pgaptmp_003747"
     CDS             1703..1951
                     /gene="ybdJ"
                     /locus_tag="ACHKNP_RS18735"
                     /old_locus_tag="pgaptmp_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HGU4705838.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWQRWFSLAD"
     gene            2016..2384
                     /gene="ybdF"
                     /locus_tag="ACHKNP_RS18740"
                     /old_locus_tag="pgaptmp_003748"
     CDS             2016..2384
                     /gene="ybdF"
                     /locus_tag="ACHKNP_RS18740"
                     /old_locus_tag="pgaptmp_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU4705839.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2478..3131
                     /gene="nfsB"
                     /locus_tag="ACHKNP_RS18745"
                     /old_locus_tag="pgaptmp_003749"
     CDS             2478..3131
                     /gene="nfsB"
                     /locus_tag="ACHKNP_RS18745"
                     /old_locus_tag="pgaptmp_003749"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HGU4705840.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3239..4486
                     /gene="ybdG"
                     /locus_tag="ACHKNP_RS18750"
                     /old_locus_tag="pgaptmp_003750"
     CDS             3239..4486
                     /gene="ybdG"
                     /locus_tag="ACHKNP_RS18750"
                     /old_locus_tag="pgaptmp_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HGU4705841.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4554..5930)
                     /gene="pheP"
                     /locus_tag="ACHKNP_RS18755"
                     /old_locus_tag="pgaptmp_003751"
     CDS             complement(4554..5930)
                     /gene="pheP"
                     /locus_tag="ACHKNP_RS18755"
                     /old_locus_tag="pgaptmp_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HGU4705842.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6032..9175)
                     /gene="cusA"
                     /locus_tag="ACHKNP_RS18760"
                     /old_locus_tag="pgaptmp_003752"
     CDS             complement(6032..9175)
                     /gene="cusA"
                     /locus_tag="ACHKNP_RS18760"
                     /old_locus_tag="pgaptmp_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HGU4705843.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9187..10410)
                     /gene="cusB"
                     /locus_tag="ACHKNP_RS18765"
                     /old_locus_tag="pgaptmp_003753"
     CDS             complement(9187..10410)
                     /gene="cusB"
                     /locus_tag="ACHKNP_RS18765"
                     /old_locus_tag="pgaptmp_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HGU4705844.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10426..10758)
                     /gene="cusF"
                     /locus_tag="ACHKNP_RS18770"
                     /old_locus_tag="pgaptmp_003754"
     CDS             complement(10426..10758)
                     /gene="cusF"
                     /locus_tag="ACHKNP_RS18770"
                     /old_locus_tag="pgaptmp_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HGU4705845.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10916..12289)
                     /gene="cusC"
                     /locus_tag="ACHKNP_RS18775"
                     /old_locus_tag="pgaptmp_003755"
     CDS             complement(10916..12289)
                     /gene="cusC"
                     /locus_tag="ACHKNP_RS18775"
                     /old_locus_tag="pgaptmp_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HGU4705846.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQETRAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSIRLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGG"
     gene            12446..13129
                     /gene="cusR"
                     /locus_tag="ACHKNP_RS18780"
                     /old_locus_tag="pgaptmp_003756"
     CDS             12446..13129
                     /gene="cusR"
                     /locus_tag="ACHKNP_RS18780"
                     /old_locus_tag="pgaptmp_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HGU4705847.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13119..14573
                     /gene="cusS"
                     /locus_tag="ACHKNP_RS18785"
                     /old_locus_tag="pgaptmp_003757"
     CDS             13119..14573
                     /gene="cusS"
                     /locus_tag="ACHKNP_RS18785"
                     /old_locus_tag="pgaptmp_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HGU4705848.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHGHMEHSNWRMINLSVGPL
                     VDGKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSV
                     SRQIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIR
                     TPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPE
                     KKMLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRY
                     TPPGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIG
                     LAIVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         3575 a         3980 c         3663 g         3588 t
ORIGIN      
        1 tccccgacca gaagcgtaaa tcccagcacc gttaaacaca gaactatgac tgccgcaagg
       61 gcatatttcg tcagcatgtt tatattgcct ccatgcgaag aagggactac catccaaatt
      121 gttcaggttt gggtaggtag ccccagggtt gatagaaata tcgtcctggg acttcatctt
      181 tccagacctc agagtaaacc tgaaaccgga aagcttcagg cacccgcagt tatcttaccc
      241 ttgcttagca aaatagctgt ttatttatac agtaagtcct gtttcggact tctcactccc
      301 catttgatca actgaagctg aacccgtttt tttctgcatg cctcaatcgt caaaattctt
      361 cgccaccagc cagccgtgcc ataacttgtc acattgcaaa agtagccaaa ttgcttcagt
      421 caccatcacc aaaaaaagaa acattccaga acttccgcat ctcgcccagc cgctccgact
      481 tctatactga atagaaaacg ccaacttaag agaaacctat gccattaccc gattttcatg
      541 tttctgaacc ttttaccctc ggtattgaac tggaaatgca ggtggttaat ccgccgggct
      601 atgacttaag ccaggactct tcaatgctga ttgacgcggt taaaaatgag atcacggccg
      661 gagaggtaaa gcacgatatc accgaaagta tgctggagct ggcgacggat gtttgccgtg
      721 atatcaacca ggctgccggg cagttttcag cgatgcagaa agtcgtattg caggcagccg
      781 cagaccatca tctggaaatt tgcggcggtg gcacgcaccc gtttcagaaa tggcagcgtc
      841 aggaggtatg cgacaacgaa cgctatcaac gaacgctgga aaactttggc tatctcatcc
      901 agcaggcgac cgtttttggt cagcatgtcc atgttggctg cgccagtggc gatgacgcca
      961 tttatttgct gcacggcctg tcacggtttg tgccgcactt catcgccctt tccgccgcgt
     1021 cgccgtatat gcagggaacg gatacgcgtt ttgcctcctc acgaccgaat attttttccg
     1081 cctttcccga taatggcccg atgccgtggg tcagtaactg gcaacaattt gaagccctgt
     1141 ttcgctgcct gagttacacc acgatgatcg acagcattaa agatctgcac tgggatattc
     1201 gccccagtcc tcattttggc acggtggagg ttcgggtgat ggataccccg ttaaccctta
     1261 gccacgcggt aaatatggcg ggattaattc aggccaccgc ccactggtta ctgacggaac
     1321 gcccgtttaa acatcaggag aaagattacc tgctgtataa attcaaccgt ttccaggcct
     1381 gccgctatgg gctggaaggc gtcattaccg atccgcacac tggcgatcgt cgaccgctaa
     1441 cggaagatac cttgcgattg ctggaaaaaa tcgccccttc cgcacataaa atgggtgcat
     1501 cgagcgcgat tgaggccctg catcgccagg tcgtcagcgg tctgaatgaa gcgcagctga
     1561 tgcgcgattt cgtcgccgat ggtggctcgc tgattgggct ggtgaaaaag cattgtgaga
     1621 tctgggccgg tgactaagta gggaattgcc ttacagaaga atatccctga caatggtgtt
     1681 ttaacttaca tttaacaatg ttatgaaaca ccctttagaa accttgacta ccgcagcagg
     1741 cattttgctg atggctttcc tctcttgcct gctgctgccc gcccccgcac tggggctgac
     1801 gctggcacaa aaactggtga ccatgttcca tctgatggat cttagccagc tttacacttt
     1861 attgttttgt ctgtggtttt tagtgctggg cgctattgag tattttgttc tgcgctttat
     1921 ctggcaacgc tggttctcgc tggcggatta aacgtcgatt atcgccgtac ttcgcggcat
     1981 actttgctta ttctccttcg cctaaaggaa cgtttatgga taagcaatca ctgcacgaaa
     2041 cggcgaaacg cctagccctt gagttaccct ttgtcgagct ttgctggcct tttggcccgg
     2101 agttcgatgt ttttaaaatt ggcggcaaaa tttttatgct gtcgtcggag ctgcgcggcg
     2161 tcccctttat caatctgaag tccgatccac aaaaatccct gttaaatcag cagatatacc
     2221 cgagcattaa gccagggtat cacatgaata aaaagcactg gatttcggtg tatcccggcg
     2281 aggaaatctc cgaagcgtta cttcgcgatc tgatcaacga ttcgtggaat ctggtggttg
     2341 atggtctggc taaacgcgat caaaaaagag tgcgtccagg ctaaagcgga aatctatagc
     2401 gcatttttct cgcttaccat ttctcgttga accttgtaat ctgctggcac gcaaaattac
     2461 tttcacatgg agtctttatg gatatcattt ctgtcgcctt aaagcgtcat tccactaagg
     2521 catttgatgc cagtaaaaaa cttaccccgg aacaggccga gcagatcaaa actctcctgc
     2581 aatacagccc atccagcacc aactcccagc cgtggcattt tattgttgcc agcacggaag
     2641 aaggtaaagc gcgtgttgcc aaatccgctg ccggtaatta tgtgttcaac gaacgtaaaa
     2701 tacttgatgc ctcgcacgtc gtggtgttct gtgcaaaaac cgcgatggac gatgcctggc
     2761 tgaagctggt tgttgaccag gaagatgctg atggccgctt tgccacgccg gaagcgaaag
     2821 ccgcgaacga taaaggtcgc aagttcttcg ctgatatgca ccgtaaagat ctgcatgatg
     2881 atgcagagtg gatggcaaaa caggtttatc tcaacgtcgg taacttcctg ctcggcgtgg
     2941 cggcgctggg tctggacgcg gtacccatcg aaggttttga cgccgccatc ctcgatgcag
     3001 aatttggtct gaaagagaaa ggctacacca gtctggtggt tgttccggta ggtcatcaca
     3061 gcgttgaaga ttttaacgct acgctgccga aatctcgtct gccgcaaaac atcaccttaa
     3121 ccgaagtgta attctctctt gccgggcatc tgcccggcta tttcccctca gattctcctg
     3181 atttgcataa ccctgtttca gccgtcatca taggctgctg ttgtataaag gagacgttat
     3241 gcaggattta atatcccagg ttgaagattt agcgggtatt gagatcgatc acaccacctc
     3301 gatggtgatg attttcggta ttatttttct gaccgccgtc gtggtgcata ttattttgca
     3361 ttgggtggta ctgcggacct tcgaaaaacg tgccatcgcc agttcacggc tttggttgca
     3421 aatcattacc cagaataaac tcttccaccg tttagctttt accctgcagg ggattatcgt
     3481 caatattcag gcggtattct ggctgcaaaa aggcaccgaa gcggcagata ttctgactac
     3541 ctgcgcgcag ttgtggatca tgatgtatgc gctgctttca gtcttctcgt tgctggatgt
     3601 tattttgaat ctggcgcaga aattcccggc ggcatctcag ttaccgctga aagggatatt
     3661 tcaggggatt aaactgatcg gcgcaatcct ggtcggcatt ttgatgatct cgctgctgat
     3721 tggtcagtcg ccagcgattc tgatcagcgg tcttggtgca atggctgccg tgctgatgtt
     3781 ggtttttaaa gatccgattc ttggtctggt ggcaggtatt cagctttccg cgaacgatat
     3841 gctgaaactg ggcgactggc tggagatgcc gaaatacggc gcggatggcg cggtgatcga
     3901 tattgggtta accaccgtca aagtgcgtaa ctgggacaat accattacca ctattcccac
     3961 ctggtctctg gtttctgact cctttaaaaa ctggagcggg atgtcagcat ctggcgggcg
     4021 acgtattaag cgcagtatca gtattgatgt caccagtatt cgttttcttg atgaagacga
     4081 aatgcaacgt ctgaataaag cgcacttgtt aaaaccttat ttaaccagcc gccatcagga
     4141 aattaatgag tggaatcgcc agcaaggttc tacggagtcg atattaaacc tgcgccgaat
     4201 gaccaatatt ggaacctttc gtgcctatct gaacgaatat ctgcgtaacc atccgcggat
     4261 tcgtaaagat atgaccttaa tggtacgcca actggctcca ggtgataacg gtttaccgct
     4321 cgagatctac gcgtttacta acaccgtggt gtggctggaa tatgaaagca ttcaggctga
     4381 tatattcgat cacatatttg cgattgtcga agagtttggt ctgcgacttc atcagtcgcc
     4441 aaccggcaat gatattcgct ctctggcggg tgcatttaag cagtaattaa aaaaaccgct
     4501 ctcatcgaat ggatgagagc ggacatcgtt ttgtcggatg tagcgtgaat gccttatttc
     4561 cgacgcagcg ttttaaatgc cacaaacagg aatacaatcc acaccggcag caggatcgct
     4621 gacaagcgca tatcatccat cgtgcacatc agcagcaaaa tcatgccgag gaaggcaatg
     4681 caaagatagt tgccgaacgg atagagcagc gccttaaact gtgtttcacg cccctgacgt
     4741 cgcatcgctg cacgaaaacg cagatgcgcc agacagatca taatccagtt caacagcagc
     4801 gttgctacca ccagcgccat cagcagacca aacgcttttt gcggcagcag atagttgatt
     4861 aataccacca gcgaagtgat cgctccggaa agcatcagcg agttaatcgg cacaccgcga
     4921 cggctgacgc gagtcaaaaa ctttggcgca ttaccctgca cagaaaggcc aaacagcatg
     4981 cggctgttag agtaaacccc gctgttatac actgacagcg atgctaccag aatgacgaag
     5041 ttcagcgcag aagctaccac gttgctgtcg agattatgga aaatcatcac aaacgggcta
     5101 ctgttggatt tcacttccac ccacggatag agcgccagta aaaccaccag tgaaccgatg
     5161 taaaacagca ggatgcgata caccacctga tttaccgctt ttggaatgct tttttccgga
     5221 tcgcgcgctt cagcggcagt aatcccaatc agctccagac cgccgaagga gaacataatt
     5281 accgccagcg acaaaatcag cccattccag ccggtggcaa agaaaccacc gtagcgccag
     5341 aggttgtcga tactggcttt ctcgccgccg tgaccagaaa acagcagcca caggccaaag
     5401 ccgatcatac cgatgatcgc cagcacttta atcagcgcaa accagaactc ggtttcacca
     5461 tataagcgca cgttcaccag gttaacggcg ttgatgataa taaagaaggc ggcagcccaa
     5521 atccacgttg gaacatccgg gaaccagtac tgcatataga tgcccgcagc ggtcagctct
     5581 gccattccca ccagcacgaa cattacccag tagttccagc cagagaggaa gcccgcaaac
     5641 ggtccccagt atttataggc aaagtgggca aatgaaccgg ataccggctc ttcgaccacc
     5701 atctcgccaa gctggcgcat aatcaggaaa gcgatgatcc cggcgacgcc gtagcccagc
     5761 aatacagccg gacccgccat ctgaatcgcc gggccaatgc caagaaacag accagtacca
     5821 attgcgccac ccagcgcaat cagttgaata tgacggttat gtaatccgcg atgaagcgtc
     5881 ggctcttgat tcgacgcagt atcttccgat acggttgacg cgtttttcac gcctttcccc
     5941 tgtgtgtctt ttttgttgag gggcaccttt taacatttag tgcccatcgt cgcaagacac
     6001 aatccacacg gttaaacggg gtatcctgct tttatttccg tacccgatgt cggtgcagcc
     6061 acatcagctt atacgccgcc gggataataa acagcgacag caaaggtgcg gtgatcatgc
     6121 cgccaatcat cggtgcggca atccggctca tcacctctga accagccccc gttccccaca
     6181 gaatcggcag cagaccggcg ataatcaccg ccaccgtcat cgctttcggg cgcacgcgca
     6241 ggaccgcgcc gtgatataac gcctcatcca gcttctgctc gctgaatgtt tgtggattat
     6301 tcaacgacgg ctcggcctct atggcgtgac gtaaatacat cagcatcacc acgccaaatt
     6361 cggcggcgac cccggcgagg gcgataaagc cagtgcccgt cgccacggaa agatgaaagc
     6421 ccatccacca cagcaaccag atgccgccca ccagcgcaaa cggtacgctg ctgataatca
     6481 gcaacgcttc gcccacccga cggaacgcca gatacaacag cacgaagata atcatcaacg
     6541 tcatcggcac catgagttta agcttatggt tggcgcgctc cagtagctcg aactgcccgg
     6601 agaatgccac gctggtgccc ggttttaact gcactttttc agctatcgct ttttgcaaat
     6661 cgtgaaccac cgacaccatg tcacgatcgc gggcatcgat ataaatccag ctcgtcgggc
     6721 gcgcattctc ggttttcagc attgacggtc cggtagagac tttaatgtcg gccacgtctg
     6781 ccagggtgat ttgctgcttc atcggcgtca ggatcggcaa ctggcgcagt gcctgcggac
     6841 tatcgcgcca gctttgcgga taacgcagat taattggata acgggcaatc ccttccaccg
     6901 tttcgccaac catcgccccg cccaccgcag aagtcacaaa caactgcaca tccgccaccg
     6961 tcataccgta acgtgcggct ttttcacggt taatctcaac gttgatatag cgcccacctt
     7021 ccagacgctc ggctagagca gaagccacgc ctggcaccgt tcgcgctact tcttcaattt
     7081 gctcagccat cgcgtcgata tccgccagca cagtgccgga aactttaatg ccgatggggc
     7141 ttttaatgcc ggttgagagc atatcgatac ggttacgaat tggcggcacc cacagattcg
     7201 ccagccccgg cagacgcacg gtgttatcca gttcctcaat gattttgtcc attgtcatgc
     7261 ctggccgcca ctgctcctgc ggcttaagct ggatggtcgt ttctaccatc tccagcggag
     7321 cagaatcggt ggcggtttcc gctttcccgg ttttgccaaa tacccgcgcc acttcaggta
     7381 cgctcataat tagcttgtcg gttttttgca gcatactcgc cgcctctgct gcggaaatcc
     7441 ccggcagcgt cgatggcata tacaacaggt cgccttcatt gatctgcggt aaaaattccc
     7501 cgccaacttt attgagcggc cagagaaccg tcagcaccga aagcgctgcc accaacagcg
     7561 tggttttcgg ccagtgcagt actttcagca acagcggatg ataaacacga atcaaaaagc
     7621 gattgagcgg gttactgctt tccggcggaa ttttgccacg gatccagtaa cccatcagga
     7681 tagggatcac tacgatcgcc agcagcgccg cacccgccat cgcatacgtt ttggtgaacg
     7741 ccagcgggcc aaacagacgc ccttcctgcc cttccagggt gaagatcggg ataaacgaca
     7801 acgtgataat cagcagactg ataaatagcg ccggacccac ttcaacagat gcatcggtga
     7861 tcacctgcca gcgcgtttta ttatccagcg tggcgtcagg atgctggtgc tgccactctt
     7921 ccagccgttt atgtgcattt tcgatcatga cgatagcggc atcgaccatc gccccgacgg
     7981 caatcgcaat gccgcccaac gacataatat tggcattcag tccctggaag tgcatgacaa
     8041 taaaagcaat acacaacccc agcggcaacg aaataatcgc caccagcgcc gagcgcacat
     8101 gccagagaaa cagcgcacag actaccgcca ccacaataaa ctcttccagc aacttgccgc
     8161 tgaggttgtc gatggcgcgg tcaatgagct ggctgcgatc gtatgttgta actatctcca
     8221 cgccttccgg cagactactt ttcagcgttt ccagtttgtc cttcacggcg gcgatcactt
     8281 ctcgggcgtt tttgccggat cgcaggatca ccaccccgcc cgccacttcg ccttcgccgt
     8341 ttagttcggc aatgccccgg cgcatctccg ggccaatctg gaccttcgca acatcgcgca
     8401 gataaacggg cacgccattt tcactggctt ttaaaacgat gtgattaaag tcgtcgagcg
     8461 tttgcagata gccgctggcg cgcaccatat attccgcttc cgccagttcg atcgacgaac
     8521 cgcccgcttc ctggtttgaa gcatccagcg cgctttttac ttcggcgaga ctgatgccat
     8581 actgcgccag gcgctgggga tcgataacca cctgatactc tttcaccaca ccgcccaccg
     8641 acgccacttc cgcaacgtca gggatggttt tcagctcata tttgagaaac cagtcctgca
     8701 atgagcgtaa atcggccaga tcgtgcttac cgctgcgatc caccagtgca tattcataga
     8761 tccagccaac acccgtggca tctggtccca gctcggcgct gactcccgca ggcagcttac
     8821 cctgtacctg gttgaggtac tccagcaccc gcgaacgcgc ccagtacgga tcggtgccat
     8881 cttcgaaaat gacatacaca taagagtcgc caaactgtga gaaaccgcgc acagtcttcg
     8941 cgccaggcac cgacaacatg gtggtggtta gcggataagt cacctgattt tcaacgattt
     9001 gtggtgcctg accgggatag ctggttttaa taataacctg cacatcggag agatccggca
     9061 gcgcatccac tggcgtatta atgatggtcc aggtgcccca gatgctcaga aacaacgcgc
     9121 ccatcagcac caaaaaacgg ttcgccaccg agcgacgaat aatccattca atcattggtt
     9181 attccctcaa tgcgcatggg tagcactttc agagcgcatc cgctccagtg cgccagaaat
     9241 attggcttcg gaatcaatca ggaacaggcc gctggaaacc accttttcac cttccgccag
     9301 accagagcgt aatgcggtga cgccttgcga tgcctggaaa acagcaacgc gtttcggtac
     9361 aaagcgcccg tcggcatcaa cggtaatcac ccgctgttcg ctgccggtat caatcagcgc
     9421 ttgtgacgga atgagcagca tcggttcgct ggcggtgttg agttgcaacc aggcgttcat
     9481 gcccggtttt agcgcctcgt cggcgttgtc gacttccaga cgcagctgca gcgtgcgggt
     9541 cgcggcatcc acgccaggta gcagcgtcca tttgcggatg gtgagtgttt tatccggtcg
     9601 cgccggaacg gtgagggtaa actgcgaggc atctttcacc agccaggcga tggactccgg
     9661 gatcgcagca gtgacccaca ccgggtccat accctgaatt ttcgcgacca cgttatcttt
     9721 ggcgatattc attcccgcgc gcagatcaaa cgcggtgatc acgccatcaa tgggcgcttt
     9781 gagcgtaaag cgagtctgga ttttttgcgt ggcgatcagg cggcgaatat ccgcctccgg
     9841 cattcccgcc agtcgcagcc gctcaagaat gccttcagtc tgggtcgccg taccgccggt
     9901 ttcgcgcagc agtaaatact cactctgcgc ttccacccag tcaggaatgg tcaggtcgag
     9961 aagcggtgtg cccttttgta ctttatcgcc cacggtaagc ggatacacct tgtcgataaa
    10021 cccggcagcg cgagcctgca caatggcata ctgatactcg ttgtaactga cattcgccgg
    10081 gaaactctgg gcaaaagtca gcggcccgcg cgtgacggta gccgttttca cccccagatt
    10141 ctgcgtctga gtcgggtcaa tgcgcacacc agacgcagaa ctctcttcat cggcatattt
    10201 cggcaccaga tccatatcca taaacggcga tttacctggt ttatcgaacc gcgtattggg
    10261 gtacatcggg tcgtaccaga ataagatttt acgttctgcg gtcgacgttt tttctgcggg
    10321 tggttcagcc tttgcaaacc aggtaaaacc tgccgcagaa ataataccgc ccgcgatcat
    10381 gctgccgata ataagcgcga tttttttcat ctcattaaac ctgggttact ggctgacttt
    10441 aatatcctgt aataaagaaa ggttgccctg ctggacaaaa ttaaacgcca ctttgtcgcc
    10501 agttttaatt tcactcattt tcgtctgcgg agtgatggta aagcgcatgg tcatctccgg
    10561 ccagttcacg gcagcaatcg gatcgtgatg gatggtgatt tttttgcttt ccagatcaat
    10621 accctttacc acgccagtgg cgctaataac ctgcggttgt acttcgctca tggtttcatg
    10681 atgatgttcg ttagcctggg cattaaagcc aataacggta aacagactga acattgcgac
    10741 ttgcagtgct tttttcattt attctctcct ggagttaaaa gtcatactct aaataattcg
    10801 agttgcagga aggcgacaag cgagtgaatc cccaggagct tacataagta agtgactggg
    10861 gtgagcgaac gcagacgcag cacatgcaac ttgaagtatg acgagtatat tgctgtcaac
    10921 cgccacctaa ggcggtatac aaagaaattt cgttaacctg acgggcataa ttcaggtcga
    10981 gtaaagtttg tcgggttgca aataaagaac gctcggcatc cagcacttcc agataactta
    11041 ctgcgccgtg ctgatataat gcccgcgccc gttgcaaagt aatttgcagc gacgccagat
    11101 aacgctgctg ggcgctgatt tgatcgttca ggctttgacg taatgccagc gcatctgcca
    11161 cttctttaaa ggcgttctgg attttctgtt cataattcac taccgactgc tgctggcgaa
    11221 tttcggcgat atccagattg gcctggttgc gtccggcatt aaaaatgggg atctcaattt
    11281 taggaataaa attccacatc ccgctgctgg cgttaaataa tgacgacaga tcgctgctgg
    11341 cggttgatat tccgctggtc agccttatag atgggaaaaa tgccgcgcgt gcggccccaa
    11401 tattggcatt agccgccatt aacgcgtgtt cagcttccat aatatccggg cgctgcaata
    11461 agatttgcga cgacaatccc gccggtaatt taacgctttg caggctgtcg ctgtttactg
    11521 tctgcgcttg cggcagcttg ccgtagcttc ccaataacag ttgcaatgca ttattcgcct
    11581 gcgccagttc cccctgacgt ttagcgatgt cgctgcgggt actttcaatc accccgcgag
    11641 cctgttccag cgccagaaca ttactgctac ctgtcaacag ttgtttttcg acaaacgcat
    11701 atgactgctg ataattacgc agcgtttctt cggctatttg caattgcgca tacgctagct
    11761 gctgattgaa ataactttgc gcgacattag aaaccagcaa aatatgcacc gcgcgctggg
    11821 cttcctcagt ggctaaataa ttttggcgct cggcttcgct catgttcttt aagcgaccga
    11881 aaaaatcgag atcaaagctg gcgttaaggc cggtcgaaaa ctcccgtgtc gtggcggtat
    11941 tccctttaag attaccgctc cagctgccgc tgccctcgcc attgagctgt gggtagcggt
    12001 cggcatcggt cagacgatat tgcgcccgcg tttcctgcac tttcagcgtc gccatgcgca
    12061 aatcccggtt attcaccagc gcctcgctaa tcagcgtctt tacctgatta tcaacaaaaa
    12121 aggtgcgcca gcccacgttc tggtagttat ctgctgcgtt aaccaagccg ttctggctga
    12181 gtgagaactg ctgcggcacg ggcattgccg gacgctgata atccggtgcc agtgaacaac
    12241 cggttagcgc aagggccaca caaaatggca gaagtttaca aggagacata ggctcataat
    12301 ttctggtgat tttataccgc caactttact cgccaggctc tgattttccg gtgacaggaa
    12361 aatgacaaaa ttgtcatttt gccaataagc gattgccatc tgatcccgct actctagaat
    12421 tgcccgggca acatgcggag gaaaaatgaa actgttgatt gtcgaagatg aaaagaaaac
    12481 cggagaatac ttgaccaaag ggttaaccga agccggtttt gtggtcgatt tggccgacaa
    12541 cgggctgaat ggctaccatc tggcgatgac cggtgattat gatctgataa tcctcgatat
    12601 tatgctgccg gacgtgaacg gctgggatat cgtgcgcatg ttgcgttccg ccaataaagg
    12661 gatgccgatt ctgttgctta ccgcgcttgg caccattgaa catcgcgtca aggggctgga
    12721 gttgggggca gatgactacc tggtgaaacc attcgctttt gctgaactgc tggcgcgggt
    12781 gcgcactctc ctgcggcgcg gggcggcggt gattatcgaa agtcagtttc aggttgccga
    12841 tctgatggtc gatctcgtca gccgcaaagt cacccgcagc ggcacgcgca tcactctgac
    12901 cagtaaagag tttactctgc tggagttttt cctccgccat cagggcgaag tgctgccccg
    12961 ctcgcttatc gcctcgcagg tgtgggacat gaatttcgac agcgacacta acgccattga
    13021 tgtagcggtg aagcggctgc gcggcaaaat cgacaacgac tttgagccaa agctaattca
    13081 gaccgtgcgc ggcgtgggtt acatgcttga ggtgccggat ggtcagtaag ccatttcagc
    13141 gcccgttttc gctggcaacc cgcctgacct tttttatcag cctggccacc atcgcagcgt
    13201 ttttcgcctt tgcatggatc atgatccact cagtaaaagt gcattttgcc gagcaggata
    13261 ttaatgattt aaaagaaatt agcgccacgc ttgaacgggt acttaatcat cctgacgaga
    13321 cgcaagcccg acgcttaatg acgctggaag atatcgtcag tggttattcc aacgtgttga
    13381 tctccctggc agatagccac ggcaaaacgg tgtatcactc ccccggtgcg cctgatatcc
    13441 gcgagtttgc acgtgatgcc atacccgata aagacgcgcg gggtggcgag gtgtatctcc
    13501 tttccggccc gacgattatg atgccaggcc acggtcacgg tcacgggcat atggaacaca
    13561 gcaactggcg gatgattaac ttgtcggttg gcccgttggt ggacggcaaa ccgatttata
    13621 cgctctacat cgcgctttcg atcgattttc atcttcatta cataaatgat ttgatgaata
    13681 aacttattat gaccgcatcg gtaatcagca tcctgatcgt ctttatcgta ctgttggcgg
    13741 tacataaagg tcacgcgccg atccgcagcg tcagccgtca aatccagaat atcacctcga
    13801 aagatctcga cgttcgcctt gacccgcaga ccgtgcccat tgagctggaa cagctggtac
    13861 tgtcgttcaa ccatatgatc gagcgtattg aggatgtctt tacccgccag tccaatttct
    13921 cagcggatat cgctcacgaa attcgcacgc cgattaccaa tctcatcacg caaacggaaa
    13981 tcgccctcag ccagtctcgc agccagaagg agctggaaga tgtgctctac tctaatctcg
    14041 aagagctgac gcgaatggcg aaaatggtca gcgatatgct gtttctcgct caggccgata
    14101 acaaccagct aatccccgaa aagaaaatgc tcaacctggc ggatgaagtc ggcaaagtgt
    14161 tcgatttttt cgaggcgtta gcggaagatc gcggcgtgga gttgcgattt gttggcgacg
    14221 aatgtcaggt tgcgggcgat ccgctgatgc tgcgtcgggc gttaagcaac ctgctttcta
    14281 atgccctgcg ttatacgccg cccggagagg caattgtagt gcgctgccag acggtcgatc
    14341 accaggtgca agttaccgtc gaaaaccccg gtacgcccat tgcgcccgag cacttaccgc
    14401 gattgtttga ccgtttctac cgcgttgacc cttcccgcca gcgaaaaggc gaaggtagcg
    14461 gcattgggct ggcgatagtg aaatcgattg ttgtcgcgca taaaggcacg gttgcagtaa
    14521 cgtcagatgc gcgggggaca aggtttgtga ttatgctgcc ggagagagag tgattattaa
    14581 gttcaaattc agggcgtggt gcatcacctc ctggtattgt tgatcaacaa attagagaaa
    14641 aacttattaa acatatgaaa tatcgaaggc ttttattttt caaagtttca cgagatgttt
    14701 gtatatgaaa cttagttaaa tctgtgtttc ttccaattgt agatcacaat gccctttcta
    14761 aattttcatc gttataaaca agccatctat catataaagc aatgaa
//
LOCUS       DAZFWL020000118        14616 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFWL020000118 DAZFWL020000000
VERSION     DAZFWL020000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.118"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..145
                     /gene="yecU"
                     /locus_tag="ACHKNP_RS18790"
                     /old_locus_tag="pgaptmp_003758"
     CDS             <1..145
                     /gene="yecU"
                     /locus_tag="ACHKNP_RS18790"
                     /old_locus_tag="pgaptmp_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HGU4705849.1"
                     /translation="AFNSTVDDTLKKQPLLFLAKLMVYSAALTLFTANFHCNMTRKIN
                     EYA"
     gene            132..356
                     /gene="yecF"
                     /locus_tag="ACHKNP_RS18795"
                     /old_locus_tag="pgaptmp_003759"
     CDS             132..356
                     /gene="yecF"
                     /locus_tag="ACHKNP_RS18795"
                     /old_locus_tag="pgaptmp_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HGU4705850.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(424..1146)
                     /gene="sdiA"
                     /locus_tag="ACHKNP_RS18800"
                     /old_locus_tag="pgaptmp_003760"
     CDS             complement(424..1146)
                     /gene="sdiA"
                     /locus_tag="ACHKNP_RS18800"
                     /old_locus_tag="pgaptmp_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HGU4705851.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(1376..2128)
                     /gene="tcyN"
                     /locus_tag="ACHKNP_RS18805"
                     /old_locus_tag="pgaptmp_003761"
     CDS             complement(1376..2128)
                     /gene="tcyN"
                     /locus_tag="ACHKNP_RS18805"
                     /old_locus_tag="pgaptmp_003761"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HGU4705852.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(2125..2793)
                     /gene="tcyL"
                     /locus_tag="ACHKNP_RS18810"
                     /old_locus_tag="pgaptmp_003762"
     CDS             complement(2125..2793)
                     /gene="tcyL"
                     /locus_tag="ACHKNP_RS18810"
                     /old_locus_tag="pgaptmp_003762"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HGU4705853.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(2808..3794)
                     /gene="dcyD"
                     /locus_tag="ACHKNP_RS18815"
                     /old_locus_tag="pgaptmp_003763"
     CDS             complement(2808..3794)
                     /gene="dcyD"
                     /locus_tag="ACHKNP_RS18815"
                     /old_locus_tag="pgaptmp_003763"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HGU4705854.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(3899..4699)
                     /gene="tcyJ"
                     /locus_tag="ACHKNP_RS18820"
                     /old_locus_tag="pgaptmp_003764"
     CDS             complement(3899..4699)
                     /gene="tcyJ"
                     /locus_tag="ACHKNP_RS18820"
                     /old_locus_tag="pgaptmp_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4705855.1"
                     /translation="MKLAHLGRQALMSVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(4787..5338)
                     /gene="fliZ"
                     /locus_tag="ACHKNP_RS18825"
                     /old_locus_tag="pgaptmp_003765"
     CDS             complement(4787..5338)
                     /gene="fliZ"
                     /locus_tag="ACHKNP_RS18825"
                     /old_locus_tag="pgaptmp_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HGU4705856.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(5384..6103)
                     /gene="fliA"
                     /locus_tag="ACHKNP_RS18830"
                     /old_locus_tag="pgaptmp_003766"
     CDS             complement(5384..6103)
                     /gene="fliA"
                     /locus_tag="ACHKNP_RS18830"
                     /old_locus_tag="pgaptmp_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HGU4705857.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(6268..7731)
                     /locus_tag="ACHKNP_RS18835"
                     /old_locus_tag="pgaptmp_003767"
     CDS             complement(6268..7731)
                     /locus_tag="ACHKNP_RS18835"
                     /old_locus_tag="pgaptmp_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HGU4705858.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRVRELTVQATNG
                     TNSDSDLSSIQAEITQRLEEIDRVSEQTQFNGVKVLAENNEMKIQVGANDGETITINL
                     AKIDAKTLGLDGFNIDGAQKATGSDLISKFKATGTDNYDVGGDAYTVNVDSGAVKDTT
                     GNDIFVSAADGSLTTKSDTNIAGTGIDATALAAAAKNKAQNDKFTFNGVEFTTTTAAD
                     GNGNGVYSAEIDGKSVTFTVTDADKKASLITSETVYKNSAGLYTTTKVDNKAATLSDL
                     DLNAAKKTGSTLVVNGATYDVSADGKTITETASGNNKVMYLSKSEGGSPILVNEDAAK
                     SLQSTTNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSRIEDAD
                     YATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            7986..9398
                     /gene="fliD"
                     /locus_tag="ACHKNP_RS18840"
                     /old_locus_tag="pgaptmp_003768"
     CDS             7986..9398
                     /gene="fliD"
                     /locus_tag="ACHKNP_RS18840"
                     /old_locus_tag="pgaptmp_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGU4705859.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDSAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            9413..9823
                     /gene="fliS"
                     /locus_tag="ACHKNP_RS18845"
                     /old_locus_tag="pgaptmp_003769"
     CDS             9413..9823
                     /gene="fliS"
                     /locus_tag="ACHKNP_RS18845"
                     /old_locus_tag="pgaptmp_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGU4705860.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            9823..10188
                     /gene="fliT"
                     /locus_tag="ACHKNP_RS18850"
                     /old_locus_tag="pgaptmp_003770"
     CDS             9823..10188
                     /gene="fliT"
                     /locus_tag="ACHKNP_RS18850"
                     /old_locus_tag="pgaptmp_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HGU4705861.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            10266..11753
                     /gene="amyA"
                     /locus_tag="ACHKNP_RS18855"
                     /old_locus_tag="pgaptmp_003771"
     CDS             10266..11753
                     /gene="amyA"
                     /locus_tag="ACHKNP_RS18855"
                     /old_locus_tag="pgaptmp_003771"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU4705862.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(11787..12200)
                     /gene="yedD"
                     /locus_tag="ACHKNP_RS18860"
                     /old_locus_tag="pgaptmp_003772"
     CDS             complement(11787..12200)
                     /gene="yedD"
                     /locus_tag="ACHKNP_RS18860"
                     /old_locus_tag="pgaptmp_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HGU4705863.1"
                     /translation="MKKLAIASALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            12387..13592
                     /gene="yedE"
                     /locus_tag="ACHKNP_RS18865"
                     /old_locus_tag="pgaptmp_003773"
     CDS             12387..13592
                     /gene="yedE"
                     /locus_tag="ACHKNP_RS18865"
                     /old_locus_tag="pgaptmp_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HGU4705864.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            13589..13822
                     /gene="yedF"
                     /locus_tag="ACHKNP_RS18870"
                     /old_locus_tag="pgaptmp_003774"
     CDS             13589..13822
                     /gene="yedF"
                     /locus_tag="ACHKNP_RS18870"
                     /old_locus_tag="pgaptmp_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HGU4705865.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13929..14597
                     /gene="yedK"
                     /locus_tag="ACHKNP_RS18875"
                     /old_locus_tag="pgaptmp_003775"
     CDS             13929..14597
                     /gene="yedK"
                     /locus_tag="ACHKNP_RS18875"
                     /old_locus_tag="pgaptmp_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HGU4705866.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNIKNQGAEL
                     IQPV"
BASE COUNT         3693 a         3648 c         3543 g         3732 t
ORIGIN      
        1 cgcatttaat agcaccgttg acgatacgct caaaaaacaa ccactgctat ttttagcaaa
       61 gcttatggta tactccgccg ccttaacatt attcaccgca aattttcatt gcaacatgac
      121 gaggaaaata aatgagtacg cctgattttt ctactgccga gaataatcaa gaactggcaa
      181 atgaagtctc ctgcctgaaa gcgatgctga cgctgatgct gcaggcgatg ggacaagctg
      241 acgcgggccg cgtgatgtta aagatggaaa aacagcttgc gctgatcgaa gacgaaaccc
      301 aggctgcagt attttccaaa acggttaagc aaattaaaca agcctaccgt cagtaatata
      361 aaaccggctg atagcgtgcc tttcagccgg tttttgcatc tggcacgcag gacagaaaag
      421 agatcaaatt aagccagtag cggccgcgta acaggcaacc tgggtcttat tcggtgcatt
      481 gatttttttc tgcatgtttt tctgatggaa attgaccgta ttctcagaga ttgacaaaat
      541 catcgctatc tctgctgatg tcttcccttc cgccgtccac ctcagaattt ctttttcgcg
      601 cttgctgaaa ttcatctctg gcgtcatcac tatttcatca tttaaacgca tcagagccat
      661 cagactttcg cgcaccagta actgcatttt taattgcagt tcatcactaa gaatgggtat
      721 ttcgcgcgcg ctgcaacggg aaaaggacaa aaagcccagc gcccggtttg gcagcattaa
      781 atactgagtg acaccgcggc gtaaaccatg tgcgcgcgcg gcttcccata acggctgtgc
      841 ttcgttgaat aagtcatcat tccacattag atggccctga ctaaagtttt cagggttcag
      901 caccggatca attgcgagaa agttttttgc ctgataataa ctaacccacg cctcagggta
      961 attggtgtag aaagccactt taggtcgcgt gaatggtacc gggtggcgaa cacataacga
     1021 atagtaatcg tactccagct gctgagcctg aagctctatt tcatggtaga cctcttctgc
     1081 ggcctccatc ctctgaaaac gcaacagcat cgtgcgacgc cagctgaaaa aatccgtatc
     1141 ctgcatagta aaccgcaacg cccctgagag tgagtatcat ttataatgat aattcaaact
     1201 atcacataaa taagatttat atataattta tattattcag gcaatgaatt acttttgcaa
     1261 gccatcacat tctcttatgt tattaatgag ttatgctgat ttgttaagca gttttatcac
     1321 gcttgaaata gcgtcccgcc ccgacaggtg aatcgtcggg gctgattttt tcttattatt
     1381 gcagcagaaa cttctcgagg aactggcggg tgcgaggctg ctggggatcg gcaaataacg
     1441 cttttgctgc cccctgctcg actatccgcc cctggtccat aaaaatcgcc cggtccgcaa
     1501 catcccgggc aaagctcatt tcgtgcgtca caatcaccat cgtgcgcttt tcctgcgcca
     1561 gctgacggat ggtgttcagg acttcgccca ccagctctgg atccagcgcc gacgttggct
     1621 cgtcaaacaa aatcacctca ggacgcattg ccagcgcacg tgcaatcgca acacgttgct
     1681 gttgaccgcc agacaaacga cgtggatagc tggtttcttt accggccagc ccaactttcg
     1741 ccaacagctc gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg acgatcaccg
     1801 gcccttcaat aatgttttcc agcaccgtac gatgcggaaa caaattaaag ttctggaaga
     1861 caaacccgac gtgctgacgc aactggcgaa tcagcgattt ttgctgactt aatgaacgtg
     1921 cagtatcaat agtgatatcg ccaacggtga tcgttcccgc ttccggttgt tccagcagat
     1981 ttatgctgcg tagcaacgtg gttttgccgg aaccactcgg accgataatt gccaccactt
     2041 cgccaggctt tacctcgagg tcgataccgt gcagcaccgt ctgaccgtga aattttttca
     2101 ccaagttctt aacttcaatg gcactcattt tggttctctc tcctggcgat taagttgatt
     2161 ctcaaaatgg ttctgcaacg tcgataatac tgtcgccatg atccagtaga tgagcgaagc
     2221 cgccaggtac atggtgaaca cctccagcgt gcgcgaggta atcaactgcg cctgacggaa
     2281 cagctccggc acctggattg tcgcggccag cgacgtatcc tttaccaggc tgataaagct
     2341 gttcgacagc ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatagt
     2401 ctgccatggc gtcataccaa tactggcagc tgcttcccac tgacctttat caatagaaga
     2461 aatggcggcg cgcagtgttt cggcggcata ggcggcagtg ttcagcgaca gaccaatcat
     2521 tgctgacgga atgggatcta attcgatacc aaactgcggc aggccgtaat agatcataaa
     2581 cagttgtgcg atgagcggtg taccacgaaa aatagagata taaaaacgcg ccagccagcg
     2641 cacgggccag attggcgaca ggcgcatcag cgcgagaata aacccgagca gtaagccaaa
     2701 aaacatgccg ccgatactga gttgcagcgt atacccagcc cctttcagca ggaacggcaa
     2761 ggaatcaata accagttgta tactttcttg catgagcgtt cccggagcta aacgtgggga
     2821 tgataggcga acagcgcagg cgcaccgccg gtatgaataa acagaatcgg cccttcatct
     2881 ttgaagcgtt tctgactgat accgtcaatc agccccgcca tcgcttttcc ggtatacaca
     2941 ggatcaagca gaatgccttc cagccgcgcc agcaatttca ctgcttccat gccttcgtcg
     3001 ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga gtataatttc cgctgatgcg
     3061 gtcagctcca gttctttcgc aatcgcctgt tgtaggttaa ccactttcgg caactgatcg
     3121 gcaacggaac gcgacacggt cacgccaatc agttcgcttt caggcatcag gtgttccagc
     3181 ccaacagcca gtccggcgtg agttccggca ctgcccgatg cgactaccac cgacgaaata
     3241 ttaaccgccc cttcacactg ttgcgcgatt tccagcgcac tctccacata acctagcgcg
     3301 ccaagagcat tagaaccgcc aaccggaatg acatacgggc gaaagccttg tgcttcgact
     3361 cgcgtcgcca gctcttccag ttgggcattg ggatcggtca gtgcttcgca catttcaatc
     3421 tgggtattga acagatccag caacaagcga ttgccgttgg ttaaatagtt ttctgcggtt
     3481 gtgccaatag gattttccag cagcgccaca cagtgcagac cgagtttcgc cgcaactgcg
     3541 gcagtctgcc gcacatggtt agactggatc gccccggcag taatcagcgt atcggcacct
     3601 tcgcgcagag catctgctgc gagaaattcc agcttacgta atttattgcc gcccattgcc
     3661 atgggggtga cgtcatcccg tttgatgaaa atttcccgtc caagataatc agaaaagcgc
     3721 ggcagatatt cgagcggtgt tggcgcgccg ataaactcca accgtggaaa acgggttaaa
     3781 ttatgcagtg gcataacagc ctccgatgtg tgttgttgtg attttcttat tatgcacgct
     3841 gaaaacgcgt aaataaaaaa ggcgctggtg aaagcgccct tttttgtcat tatgctgatt
     3901 atttggtcac atcagcacca aaccattttt cggaaagggc ttgcagagtg ccatcttttt
     3961 gcatttccgc aattgcatcg ttcactgctt tcagcaggtc ttcatttcct ttacgcagcg
     4021 ccacgccaga ctcctgacgg gagaatgctt caccggttac tgccagcgta tcgttggttt
     4081 tcttcaccag atccagcgcc gccagacggt caacgaggat cgcatcgata cggcctacgc
     4141 gcagatcctg atatttggtc gggtcatcat cataggtacg cacatcgacg ccctgaacat
     4201 tctgccgcag ccactcttca tagttggtgc ccagaccgac acccactttt ttgcctttca
     4261 gatcagcagc cgttttaatg gtgccttcgt tacctttttt caccagcgcc tgaataccag
     4321 aaatggtgta cggggtagag aaatcgtatt ttttcttgcg ctcatcagaa atggtgacct
     4381 gattaatcac cacatcaatg cgtttagagt ccagcgacgc cagcataccg tcccatttgg
     4441 tcggttttag tgacgcctca acgccaagat gttttgccag ctgttgggca aattccactt
     4501 caaaaccggt taatttgccg tcatccccct gaaaactgaa cggcggataa gtgccttcca
     4561 gccctaccag cagcgtgccg cgctctttaa ctttattaag cagaccttca tctgcaaaac
     4621 ttttaacgct catgcccgca accagcgcta cggccatcac actcatcaat gcctgacgtc
     4681 ccagatgtgc taatttcata tttaccccga atgttgttat gtctgtttgc agtgtagagc
     4741 cattgtttgt aaacacaaaa acaactccgc tacatcttat tcttatttaa tatatatcag
     4801 aagcaggcag gctggaggat ttctgcacaa gtccggtaca cgtttgacgc tgatagtgtt
     4861 gatacttccg taatgcgatg cggtaattgt tggtgctggt ggtgggcagc cacggggcga
     4921 ggatttcatc aaggaaaccg tcctgcagcg gatcgaggga aatattttgc tcgtgtagat
     4981 gattccccaa acggcgcaaa cgaacgacat attcacgcac cgtacctggg ctcatttcag
     5041 tttgctcaaa aagaaattgc ttaaagccga taatatcgaa aaaatcactc tgcgttttgc
     5101 aatgtaaatc accgcaaaat cggcacaatg ccgcccatga tttttgttcc gtttgccagc
     5161 cattttcatc aagcagcgcg tccaggcggg aaatctcgat tttattcact attttgccgt
     5221 cgcgaacgag ggtaatgcga tcgagtaatt tacggcaatg cgcgcaatga gtctggctgt
     5281 gtttaaagtc tttaagatag cggcttaatg gccgtctttt caggtgctgc accatcatta
     5341 agaactcctg gtagtcaaag ttaaagtgcg gcatttactg acgttataac ttacccagtt
     5401 tagtgcgtaa ccgtttaata gcctggctgt gtaactgact gacccgcgat tccccgacct
     5461 ccagcaccgc gccaatctct ttgagattca gctcttcctg gtaatagagg gttaatacca
     5521 gtttttcgcg ctccggcaac gtttcgatgg cttccatcac ccgctgacgc agattactgt
     5581 ccagcagttg ttgtagcggg ttttctcgct gatgatcatc agtaaccagt tcgatgctat
     5641 cgccgtgctc ttcgcgccac tcatcgtagg agaagagctg gctgttattg gtgtcgagca
     5701 acatttggcg ataatcggca atatcgatcc ctaaacgttc cgctacctca gtttccgtgg
     5761 cgttgcggcc aagttcctgc tccagttgcc ctattgcctg tgccacttca cgcgcgttgc
     5821 gtcgcacgct gcgcggcacc cagtcacggc tgcgaagttc atccagcata gcgccacgga
     5881 tacgctgcac tgcgtaagtt gtaaatgccg ttccttgtag ggcgtcatag cgttcgacgg
     5941 cattaagtaa cccaatgccg cccgcctgta gcagatcgtc aagttccacg ctcgcgggca
     6001 gtcgaacctg caggcgcaat gcttcgtgac gcaccagcgg gacataacgc tgccatagcg
     6061 agtgtttatc cattacacct tcagcggtat agagtgaatt cacgataaac agccctgcgt
     6121 tatatgagtt atcggcatga ttatccgttt ctgcagggtt tttaatcgga cgattagtgg
     6181 gtgaaatgag gggttatttg ggggttaccg gtaaattgcg ggcagaaaaa accccgccgg
     6241 tggcgggggt tgagcgataa gtgtaaatta acgcagcaga gacagtacgt tctgcgtggt
     6301 ctggttagcc tgcgccagaa cagaggtacc cgcttgttgc aggatctgcg cacgagacat
     6361 gttagacact tcggtcgcgt agtcagcatc ttcgatacgg ctacgggcag aagacaggtt
     6421 gtttacggtg ttgccaaggt tggtgatagc agagtcgaaa cggttttgta ctgcaccgag
     6481 gtcagaacgc agattgtcaa ctttagccaa tgctttgtcg atagtttcga gcgggttggt
     6541 ggtagattgc aacgattttg ctgcatcttc gtttaccaga atcgggctac caccttctga
     6601 tttgctcaga tacatgactt tattgttacc agaagcagtc tccgttatcg ttttaccatc
     6661 tgcactaaca tcgtaagttg caccgttaac aactaacgtg cttcctgttt tcttagctgc
     6721 attgagatca agatcggaaa gtgtggcagc cttgttatca actttggttg tcgtataaag
     6781 gccagcgcta tttttgtaaa ctgtctcact cgtaatcaaa gaagcttttt tgtcagcatc
     6841 tgtcacagta aatgtcactg acttaccatc aatttctgca gaatatacac cattcccatt
     6901 gccatccgct gcagttgttg ttgtgaattc aactccatta aacgtgaatt tatcattctg
     6961 tgctttattc ttagccgctg ctgcgagtgc tgtagcatca atccctgtac cagctatgtt
     7021 tgtgtcagat ttagttgtca gtgaaccatc tgctgcacta acaaaaatat cattccctgt
     7081 agtatcttta acagctccgc tatctacgtt aacagtataa gcatcaccgc caacatcata
     7141 gttatcagta cctgtcgctt taaatttaga aatcaggtca ctgccagttg ctttctgcgc
     7201 gccatcgata ttaaaaccgt ccaggccgag agttttcgca tcaatttttg ccagattgat
     7261 agtgatggtt tcaccatcat tagcaccaac ctgaattttc atttcattat tttcagcaag
     7321 gactttcacg ccgttaaact gagtttgctc agatacacgg tcaatttctt ccagacgttg
     7381 agtaatttca gcctggatag aagaaagatc gctgtcagag ttagtaccgt tagttgcctg
     7441 aacagtcagt tcacgtacac gctgcaggtt gttgttaatt tcattcagcg caccttcagt
     7501 ggtctgcgca acagaaatac catcattcgc gttacgggaa gcctgagtca gacctttaat
     7561 atttgctgta aaacggttag caatcgcctg acctgctgcg tcatctttag cactgttaat
     7621 acgcaggcca gaagagagac gttcaatggc ggagctcaga gaagactgag atttgttcag
     7681 gttattctgg gtcaacagcg acaggctgtt tgtattaatg acttgtgcca tgatcttttc
     7741 cttatcaatt acaacttgat gttattgggc tgttgcccac ggtttctcac cgtacccctt
     7801 gtatcggcac ctgaatttcg aactttagaa aattttagtt tctctcctgt tttttcttaa
     7861 cggctgaaat atccgtaata tacaactcta ctctgcgcat catcttcgtg aatcaactat
     7921 taaactttgc gcacaaattg ccgataaccc gcttatctac tgtttgcaat caaaaggaat
     7981 taggtatggc ttcatttaca tctcttggcg ttggttcgaa tctgccgctg gatacattgc
     8041 taaacaacct gaccatcgct gaaaaaaaac gcttaaaccc aattactcag caacagagtg
     8101 acaataccgc acgtctgaca gcctatggca cattaaaaag tgcattagaa aaattccaga
     8161 ctgcaaatac tgcgttaaat aaagccgatc tgttcaaaag cactaccgtt accagtagca
     8221 atgaagacct taaagtttcc accgaagcag gtgctgcccc cggtatctat accatcagcg
     8281 taacccaact ggcgcaggcg caatctttac gaactgattc cccgacaatc attgcatcaa
     8341 caaaagatgc actgggtgat gagtcatcag atacacgcac aataaaaatt acccaagacg
     8401 gtcgtaaaga gccgctggaa atcaagctca ataaagatca gacgtcattg gatgagatca
     8461 gcaaagccat taacgatgcc gatagtggaa tttccgctag tatcgttaaa gtcaaagatg
     8521 gtaactatca attggtattg accgccagtg aaggtctggc caataaaatg accatatctg
     8581 ttgagggtga cagtaaatta aatgatctgc tggcttatga cagcaagacc aacaccggga
     8641 atatgaagga actggttaac gcgcaaaatg cacagctaaa cgttaacggt atcgatattg
     8701 aacgttcaag taacaaaatt accgatgctc ctcagggagt gactctcgat ttgaccaaaa
     8761 aagtcactga cgtccgggtc acggtgacaa aaagcaacga taaagcaacc gaagcaatta
     8821 aaggctgggt ggattcatat aactccctca ttgacacctt taacaccctg acaaaatata
     8881 aagaagtaga cccaggtgct gaagcacaag ataaaaataa tggtgcactt ctcggagaca
     8941 gcgttgtacg tacaattcaa agcggcatcc gcgcacaatt tgcaaacggt gccagcgaca
     9001 gtgccttcaa aacattaaac gagatcggga ttaagcagga cggcacgacg ggtaagctga
     9061 aaattgatga tgataaactc aaaaaagtcc tgaatgaaaa tacagcatcc gtacgtgaat
     9121 tgctggtagg cgatggtaaa gaaacaggaa ttaccaccaa aattgccacc gaggtaaaag
     9181 gttaccttgc cgatgatggc attattgata gcgcacagga cagtattaac gccacactga
     9241 aaaaactgac caaacagtat ctatccgtca gtgccagcat tgatgacacc gtggcgcgtt
     9301 acactgcaca gtttacgcaa ttagatacca tgatgagcaa actgaataac accagtacct
     9361 atctcagcca acaatttact gcaatgagta attcctgatc acggaggtca gcatgtatac
     9421 cgcgagcggc tcaaaagcct atgcacaaat tggcgtcgaa agcgccgtaa tgagcgccag
     9481 ccagcagcag ctggtcacca tgctatttga tggagtgctg agcgcactgg ttagagcacg
     9541 cctgtttatg caggacaaca atcagcaagg caaaggcgtc tctttgtcaa aagcgatcaa
     9601 catcattgag aacggactgc gggtgagtct tgatgaagag agcaaagacg aattaaccca
     9661 aaacttgatt gctctttata gctatatggt caggcgcttg ctgcaagcca atttacgcaa
     9721 cgatgtctcc gcagtcgaag aagtggaagc attaatgcgc aatattgccg atgcctggaa
     9781 agagtcgtta ctctcccctt ctttgattca ggacccagtc tgatgaacca cgcaccgcat
     9841 ttgtatttcg cctggcaaca actcgtcgaa aaaagccagc tcatgttacg cctggcaaca
     9901 gaagaacaat gggacgaact catcgccagc gagatggcgt atgtgaatgc ggtgcaggag
     9961 attgcacatc tgactgaaga gattgacccg tccaccacga tgcaggagca gctccgcccg
    10021 atgctgcgcc tgattctcga caacgaaagc aaggtaaagc agttattaca gattcggatg
    10081 gatgaactgg cgaaactggt cggtcagtca tcggtgcaaa aatcggtgtt aagtgcctat
    10141 ggtgatcagg gcggctttgt gctggctccg caggataacc tcttttgaat ctgaacgagt
    10201 cgatggctcg cgaataatcc gattacggct acgcttctaa tgttcccctt gaatggagtc
    10261 gaagaatgcg taatcccacg ctgttacaat gttttcactg gtattacccg gaaggcggta
    10321 agctctggcc tgaactggcc gagcgcgccg acggttttaa tgatattggt atcaatatgg
    10381 tctggttgcc gcccgcctat aaaggcgcat cgggcgggta ttcggtcggc tacgactcct
    10441 atgatttatt tgatttaggc gagtttgatc agaaaggcag catccctact aaatatggcg
    10501 ataaagcaca actgctggcc gccattgacg ccctgaaacg taatgacatt gcggtgctgt
    10561 tggacgtggt agtcaaccac aaaatgggcg cggatgaaaa agaagctatt cgcgtgcagc
    10621 gtgtaaatgc tgatgaccgt acgcaaattg acgaagaaat cattgagtgt gaaggctgga
    10681 cgcgttacac cttccccgcc cgtgccgggc aatactcgca gtttatctgg gatttcaaat
    10741 gttttagcgg tatcgaccat atcgaaaacc ctgatgaaga tggcatattt aaaattgtta
    10801 acgactacac cggcgaaggc tggaacgatc aggttgatga tgaattaggt aatttcgatt
    10861 atctgatggg cgagaatatc gattttcgca atcatgccgt gacggaagag attaaatact
    10921 gggcgcgctg ggtgatggaa caaacgcaat gcgacggttt tcgtcttgat gcggtcaaac
    10981 atattccggc gtggttttat aaagagtgga tcgaacacgt acaggaagtt gcgccaaagc
    11041 cgctgtttat tgtggcagag tactggtcgc atgaagtcga taaactgcaa acgtatattg
    11101 atcaggtgga aggcaaaacc atgctgttcg atgcgccgct gcagatgaaa ttccatgaag
    11161 catcgcgcat ggggcgcgac tacgacatga cgcagatttt cacgggtaca ttagtggaag
    11221 ccgatccttt ccacgccgtg acgctcgttg ccaatcacga cacccaacca ttgcaagccc
    11281 tcgaagcacc ggtcgaaccg tggtttaaac cgctggcgta tgctctaatt ttgttgcggg
    11341 aaaatggcgt tccttcggtg ttctatccgg acctctacgg tgcgcattac gaagatgtcg
    11401 gtggtgacgg gcaaacctat ccgatagata tgccaataat cgaacagctt gatgagttaa
    11461 ttctcgcccg tcagcgtttc gcccacggtg tacagacgtt atttttcgac catccgaact
    11521 gcattgcctt tagccgcagt ggcactgacg aatatcccgg ctgcgtggtg gtcatgtcga
    11581 acggggatga tggcgaaaaa accattcatc tgggagagaa ttacggcaat aaaacctggc
    11641 gtgattttct ggggaatcgg caagagagcg tagtgaccga cgaaaacggc gaagcgacct
    11701 tcttttgcaa cggcggcagc gtcagcgtgt gggttatcga agaggtgatt taaattcatc
    11761 cccggcggca agccggggag atttcattac ggcagtggcg tcggtaacgg cgctttatcc
    11821 agcgcggcgg cacactctgc cgtcgggcga tccacacgct ccatcgtcat accatcgtac
    11881 tcaatggtgt taccgtcacg ctcaacctcg tacagctcac gttttacggt aacgttagtg
    11941 agatcatcag acatcaatgt caacttgccc ggcacggcaa tcacccgctg ccactgacgg
    12001 caatccaggg tatcgccctc tttagtgaca atcaggctgc ctatcgcttc cggactgacc
    12061 agcgcacgtt gcggcccttt ggtctgccag tagcccgcta gccagtccgg cgcgggggtt
    12121 ttcacgacat tgttatagtt ttcgacttcg gcacagcccg ccagcaacat gagcgcgctt
    12181 gcaattgcga gttttttcat cattcgtcct gcatgagaag aaaagaggat tgtggcatta
    12241 aagccctgat gtcgccagcc tttccaggtt cagagagaaa tttgatctaa ctattatttt
    12301 tgtgaattat taccccgaaa ataaatttgc atgatgtatg attcgcgatt cttgtttcaa
    12361 accttctgag ttcagaggct acattcatgt catggcagca attcaaacac gcctggttga
    12421 ttaaattctg ggcacccatc cctgcggtca tcgcggcggg tattctctcc acttactatt
    12481 ttggcattac tggcaccttt tgggctgtca cgggtgaatt tacccgttgg ggcggccagc
    12541 tcctgcagct gtttggcgtc catgctgaag agtggggtta ttttaaaatt atccatctgg
    12601 aaggatcgcc attaacccgc atcgacggga tgatgatcct cggcatgttt ggcggctgct
    12661 ttgccgcagc gctgtgggcc aacaatgtca aactgcgcat gccacgcagc cgtatccgca
    12721 ttatgcaggc cattattggc ggtattatcg ctggttttgg cgcgcgtctg gcaatgggct
    12781 gtaacctggc ggcgttcttt accggtattc ctcagttctc gctgcatgcc tggttctttg
    12841 ccatcgccac tgccattggt tcatggtttg gcgcacgctt taccctgctg cccatcttcc
    12901 gtattcccgt gaaaatgcag aaagtttctg ccgcctcacc gctgacgcaa aaaccggatc
    12961 aggcgcggcg tcgttttcgt ctcgggatgc tggtcttttt cggtatgctg ggctgggcgc
    13021 tgctcacggc gatgaaccaa ccaaaactgg ggctggcaat gctgtttggc gtcggctttg
    13081 gtttactgat tgaacgtgcg caaatctgct ttacttcggc gttccgcgat atgtggatca
    13141 ccggacgtac ccatatggcg aaagcaatca ttatcggtat ggcggtgagt gccatcggga
    13201 tcttcagtta cgtacagtta ggcgttgaac ccaaaatcat gtgggcggga ccaaacgcgg
    13261 taattggtgg tttactgttt ggttttggca tcgtgctggc aggcggctgc gaaaccggct
    13321 ggatgtaccg cgcggtagaa ggccaggtgc actactggtg ggtcggtctg ggcaatgtga
    13381 tcggctcgac gattctggcg tattactggg atgatttcgc tccggcgctg gccaccgact
    13441 gggacaaaat caacctgctg aaaacctttg gcccgatggg tggcctgctg gtgacatatt
    13501 tgctgttgtt tactgcgctg atgttgatta tcggctggga aaaacgcttc ttccgccgtg
    13561 cggcaccgca gactgctaag gagatcgcat gaaaaatatc gttcctgatt accgccttga
    13621 tatggtgggt gaaccctgcc cttatccggc agtcgcaact cttgaggcga tgccgcagtt
    13681 aaaaaaaggg gaaatcctgg aagtggtgag cgactgtcca cagtcgatca ataatattcc
    13741 actggatgcg cgtaatcacg gctatacggt gctggatatt cagcaagacg ggccgaccat
    13801 tcgttattta attcagaagt aatccctcat tcccgctgtg gcccacagcg ggaacatcgt
    13861 tgcctctgac acatagattt tacccgcccc ctctcctatt cttttcagta tgatctcagg
    13921 aggtttctat gtgtggacgc tttgcccaat cccaaacgcg tgaagattac cttgcgcttc
    13981 tcgcggaaga tattgaacgc gatattccct acgatcccga acccattggc agatacaacg
    14041 tcgcgccggg aaccaaagtt cttctgctca gtgaacgtga tgaacacctt catctggatc
    14101 cggttttctg gggatatgct cccggatggt gggataaacc gccgctgatt aacgcccgcg
    14161 tagaaactgc ggccaccagt cgtatgttta aaccgctctg gcaacatggt cgggcaatct
    14221 gttttgccga tggctggttt gagtggaaaa aagaaggcga caaaaaacag ccttatttta
    14281 tctatcgcgc tgatggacaa cctgttttta tggccgcgat aggtagcaca ccgtttgagc
    14341 gcggtgacga agccgaagga tttttaattg tcactgcagc agcagaccag ggtctggtag
    14401 atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcacaggaa tggatgcggc
    14461 aagagattgg cggtaaagaa gcatcagaaa ttgctaccaa cggctgtgtg ccagcaaacc
    14521 agttcacctg gcacccggta tcgcgcgcgg tgggtaatat caaaaaccag ggagcggagt
    14581 taattcaacc ggtttgataa gccaggagac tattga
//
LOCUS       DAZFWL020000119        14421 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFWL020000119 DAZFWL020000000
VERSION     DAZFWL020000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.119"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            20..586
                     /locus_tag="ACHKNP_RS18880"
                     /old_locus_tag="pgaptmp_003776"
     CDS             20..586
                     /locus_tag="ACHKNP_RS18880"
                     /old_locus_tag="pgaptmp_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4705867.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            637..3096
                     /locus_tag="ACHKNP_RS18885"
                     /old_locus_tag="pgaptmp_003777"
     CDS             637..3096
                     /locus_tag="ACHKNP_RS18885"
                     /old_locus_tag="pgaptmp_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4705868.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNDVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3111..3842
                     /locus_tag="ACHKNP_RS18890"
                     /old_locus_tag="pgaptmp_003778"
     CDS             3111..3842
                     /locus_tag="ACHKNP_RS18890"
                     /old_locus_tag="pgaptmp_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4705869.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNIGNYSGSQFYLGYMD
                     DYGALRMTTLNYSGQCHLQAVEAKK"
     gene            3839..4900
                     /locus_tag="ACHKNP_RS18895"
                     /old_locus_tag="pgaptmp_003779"
     CDS             3839..4900
                     /locus_tag="ACHKNP_RS18895"
                     /old_locus_tag="pgaptmp_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4705870.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5052..6098
                     /locus_tag="ACHKNP_RS18900"
                     /old_locus_tag="pgaptmp_003780"
     CDS             5052..6098
                     /locus_tag="ACHKNP_RS18900"
                     /old_locus_tag="pgaptmp_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGU4705871.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6095..6886)
                     /gene="nei"
                     /locus_tag="ACHKNP_RS18905"
                     /old_locus_tag="pgaptmp_003781"
     CDS             complement(6095..6886)
                     /gene="nei"
                     /locus_tag="ACHKNP_RS18905"
                     /old_locus_tag="pgaptmp_003781"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HGU4705872.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNELTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERVLSPRFRNRQFAGLLLE
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6922..7656)
                     /gene="pxpA"
                     /locus_tag="ACHKNP_RS18910"
                     /old_locus_tag="pgaptmp_003782"
     CDS             complement(6922..7656)
                     /gene="pxpA"
                     /locus_tag="ACHKNP_RS18910"
                     /old_locus_tag="pgaptmp_003782"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HGU4705873.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7646..8578)
                     /gene="pxpC"
                     /locus_tag="ACHKNP_RS18915"
                     /old_locus_tag="pgaptmp_003783"
     CDS             complement(7646..8578)
                     /gene="pxpC"
                     /locus_tag="ACHKNP_RS18915"
                     /old_locus_tag="pgaptmp_003783"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HGU4705874.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGSGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8572..9228)
                     /gene="pxpB"
                     /locus_tag="ACHKNP_RS18920"
                     /old_locus_tag="pgaptmp_003784"
     CDS             complement(8572..9228)
                     /gene="pxpB"
                     /locus_tag="ACHKNP_RS18920"
                     /old_locus_tag="pgaptmp_003784"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HGU4705875.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9251..9994)
                     /gene="ybgI"
                     /locus_tag="ACHKNP_RS18925"
                     /old_locus_tag="pgaptmp_003785"
     CDS             complement(9251..9994)
                     /gene="ybgI"
                     /locus_tag="ACHKNP_RS18925"
                     /old_locus_tag="pgaptmp_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HGU4705876.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10265..11746
                     /gene="dtpD"
                     /locus_tag="ACHKNP_RS18930"
                     /old_locus_tag="pgaptmp_003786"
     CDS             10265..11746
                     /gene="dtpD"
                     /locus_tag="ACHKNP_RS18930"
                     /old_locus_tag="pgaptmp_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HGU4705877.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11788..13206)
                     /gene="phrB"
                     /locus_tag="ACHKNP_RS18935"
                     /old_locus_tag="pgaptmp_003787"
     CDS             complement(11788..13206)
                     /gene="phrB"
                     /locus_tag="ACHKNP_RS18935"
                     /old_locus_tag="pgaptmp_003787"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HGU4705878.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVKTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13203..13712)
                     /locus_tag="ACHKNP_RS18940"
                     /old_locus_tag="pgaptmp_003788"
     CDS             complement(13203..13712)
                     /locus_tag="ACHKNP_RS18940"
                     /old_locus_tag="pgaptmp_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HGU4705879.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13863..14421)
                     /locus_tag="ACHKNP_RS18945"
                     /old_locus_tag="pgaptmp_003789"
                     /pseudo
     CDS             complement(13863..14421)
                     /locus_tag="ACHKNP_RS18945"
                     /old_locus_tag="pgaptmp_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3473 a         3853 c         3508 g         3587 t
ORIGIN      
        1 tctgttaaag gactccgtta tgtttaaagg acaaaaaaca ttggccgcac tggccgtatc
       61 tctgctgttc acagcacctg tttatgcggc tgatgaagga tccggtgaaa ttcactttaa
      121 aggtgaagtt attgaagcac cgtgtgaaat acatcaggat gatattgata aagaggttga
      181 actcggtcag gtgaccacca gccacattaa tcagtcacat cacagcgatg ccgttgctgt
      241 cgacctgcgc ttagtcaact gtgatctgga aaactccagc aacggttccg gtggcaagat
      301 ttctaaagtt gcagtcacct ttgatagctc agcgaaaacc accggcgcag atccgattct
      361 caacaacacc agcacgggtg aagccaccgg cgtcggcgta cgtttaatga ataaagatca
      421 aagcaatatc gttttaggca ccgctactcc agatatcgac ctggctccga cctccagcga
      481 acaaacgctg aatttctttg cctggatgga acaaattgat caggctacac ctgtaacgcc
      541 aggcgcagtt acggcgaatg caacctacgt gctggattat aaataagtat tactttggcg
      601 gcgctatcgc cgcctttcat aaaatattta tcaggtatgg acacagtgaa tatttatcga
      661 ctctcttttg tatcctgcct ggtcgtggcg atgccttgcg cattggcggt cgaattcaac
      721 cttaatgttc tcgataaatc aatgcgcgac cgcattgata tttcattatt aaaggaaaaa
      781 ggagtcattg ctcccggtga gtattttgtt agcgttgcgg taaataataa ccaaatcagt
      841 aacgggcaaa agattaactg gcacaaaaat gacgataaaa ccattccgtg catcaatgat
      901 ttactggtcg ataaatttgg cttaaaacct gaagtccgtc agtcgttacc attgataaat
      961 cagtgcgtcg attttagctc ccgacctgaa atgctcttca atttcgatca agccaatcag
     1021 caactaaata tcaccattcc gcaagcctgg ctggcgtggc actcagaaaa ctggacccca
     1081 ccctccacat ggaaagaagg tgtcgccggt gtcctgatgg attacaactt gtttgccagc
     1141 agctaccgcc cacaggatgg cagcagcagc actaatctta acgcctatgg aacagccgga
     1201 atcaacacgg gggcatggcg cttacgcagt gattatcagt tgaatcagac tgatagcgat
     1261 gataaccatg aacagtcagg cgaaatatca cgtacctatc tttttcgccc gttaccgcaa
     1321 ttaggttcaa aattaaccct cggcgaaacg gattttagtt ccaatatttt cgacggtttt
     1381 tcttataccg gcgcggcact ggcaagtgac gatcgaatgt tgccatggga actacgcggc
     1441 tacgccccac aaattagcgg tattgcacag accaatgcca cggtgacgat cagtcaatca
     1501 ggccgcgtca tttaccagaa aaaagtccca ccaggcccat ttatcattga cgaccttaat
     1561 cagtctgttc agggcacact ggatgtcaaa gtgacggaag aagatggtcg ggtgaacaat
     1621 ttccaggttt cggcagcatc gacgcccttc ctgactcgtc agggacaggt tcgctataaa
     1681 ctggccgcgg gtcagccacg gccctccatg tcacatcaaa ctgaaaatga aacctttttt
     1741 agcaatgatg tttcctgggg gatgctgtca aacacctcgc tgtacggcgg cctgctgctt
     1801 tctggtgatg actaccattc tgccgcaata ggtatcgggc aaaatatgct gtggcttggc
     1861 gcgctgtcgt ttgatgtcac gtgggccagt agtcactttg atactcagca ggacgagcgg
     1921 ggcttaagct accgttttaa ttacagcaaa caagtggatg ccaccaacag cacgatttcc
     1981 ctcgccgctt atcgtttctc cgatcgtcat tttcacagct acgccaacta tcttgatcac
     2041 aaatacaacg acagcgatgc gcaggacgaa aaacagacga tcagcttatc tgtgggccaa
     2101 ccgattaccc cactaaacct caatctttac gccaacctgc tacatcaaac ctggtggaat
     2161 gcagacgcct ccacgaccgc caacatcacg gcaggtttta atgttgatat tggtgactgg
     2221 agagatatct ctatttcgac gtcattcaat acaacccatt acgaagataa agatcgcgac
     2281 aaccagattt acctgtcgat ttcgctcccc ttcggtaacg gtggtcgggt tggctatgac
     2341 atgcaaaaca gtagccacag caccacacac cgcatgtcgt ggaacgatac gctggatgaa
     2401 cgtaatagct ggggcatgtc tgccggactg caatccgacc gtccggacaa tggagcccag
     2461 gtgagcggta actatcagca tctgagttca gcgggtgagt gggatatttc tggtacctat
     2521 gccgccaatg attacagttc cgtcagcagc agctggagcg gttctttcac cgcaacccaa
     2581 tatggtgcag cgtttcatcg ccgcagctcc accaatgaac cacgcctgat ggtcagcacc
     2641 gatggcgtgg cagatattcc ggttcagggc aatctcgact acaccaacca ttttggcatt
     2701 gcggtggtgc cgttgatttc cagttaccag ccttccaccg tggcggtgaa catgaatgac
     2761 ttacccgacg gcgtaacagt tgcagaaaac gttatcaaag aaacgtggat tgaaggcgcg
     2821 ataggttaca aatcactggc ttcccgttcc ggtaaagacg ttaacgtcat cattcgcaac
     2881 gccagcggtc agtttcctcc cctcggagcg gatatccgcc aggatgacag cggcattagc
     2941 gtggggatgg ttggcgagga aggacatgcc tggttaagcg gagtcgctga aaatcaaaag
     3001 tttaccgtgg tctggggtga tagccagcat tgctcgctcc atcttcctga acatatggaa
     3061 gacaccgcaa atcgcctgat tttaccttgt cattaattaa ggaaatgaaa atgacattta
     3121 tgaaaggact accccttttg ttgttggttg ccagtctgtg cagccacgct gcactacaac
     3181 ccgatcgcac tcgtattgtg tttaacgcta atgataaagc taccagtctg cgggtggata
     3241 atcgtagcga taaactcccc tacctcgctt attcctggct cgaaaatgaa aaaggggaga
     3301 aaagcgatga tcttctggtc gccctgccgc cgatacaacg tctggaaccg aaagcaacaa
     3361 cgcaggtacg gatagtaaag caagcctcga ccacaaaatt accgggcgat cgcgaaacgc
     3421 tatttttcta caatatgcgt gaaattcctc ccgccccgga aaaaaacagc gaccatgccg
     3481 ttctgcaagt cgccattcag agtcgtatca aggtgttctg gcgaccggct gcattacgca
     3541 agaaagcagg agaaaaggtc gaactgcagt tacaggtcag ccagcagggc aaccaactga
     3601 cactgaaaaa tcctaccgcg tattatctga ccattgccta cctgggacga aatgaaaaag
     3661 gcgttctccc cggcttcaaa accgtgatgg ttgcgccttt tagtacggtc aacacaaaca
     3721 tcggaaatta tagcggcagc cagttttatc tcggttatat ggatgattac ggcgcgttac
     3781 gcatgacgac gctcaactac agcggacaat gccatttaca agccgtggag gcgaagaaat
     3841 gagtgctggc aagggagtgt tgctcgtcat ctgtctgtta tttctgccgt tgaagtctgc
     3901 gcttgccctg aactgctact ttggttcatc cggtggctcg gtagaaaaat cagaagcgat
     3961 tcaacctttt gctgtaccag gcaatgccaa actcggcgat aagatttggg aatctgacga
     4021 tattaaaatt cccgtctatt gtgacaacaa taccaacggc aattttgaaa gtgagcatgt
     4081 ttatgcctgg gtaaacccct acccgggggt gcaggatcgc tattatcaac tcggcgtaac
     4141 ttacaatggc gttgattatg acgccaacca gggaaaaagc cgcatcgaca ctaaccagtg
     4201 tattgacagt aagaacattg acatttatac ccctgagcag attatcgcga tgggatggca
     4261 gaacaaaatt tgctcgggtg atcccgctaa tattcacatg tcacgtacct ttctcgctcg
     4321 catgcggtta tacgtcaaaa tacgagaaat gccgccgcat gattatcaaa gtacgcttag
     4381 cgactatatc gtcgtgcaat ttgacggtgc cggtagcgtt aacgaagatc ctactgccca
     4441 aaacctgaaa tatcatatta ccggtctgga aaacattcgc gtgctggatt gcagcgtcaa
     4501 tttttccatt tcaccggaaa cacaggtgat tgattttggt aaatttaatt tgctggatat
     4561 tcgtcgccac acaatgtcga aaacattcag tattaagacg acaaaatcac aaaatgacca
     4621 atgcaccgac ggatttaaag tcagttcctc cttttatacc gaagaaacgt tggttgaaga
     4681 agataaagca ttgttgattg gtaatggtct gaagctacgt ttgctggatg aaaacgcctc
     4741 gccttatacc ttcaataaat atgctgaata cgccgatttc accagcgaca tgttggtcta
     4801 cgaaaaaacc tatacggctg aactttcgtc cattgcaggt acccccatcg aggctgggcc
     4861 cttcgataca gtggtgcttt ttaaaattaa ctataactga tttgccatga tgccctttgc
     4921 agccggaaag ggcatctctt tcagaaaatt gctgattttc cccttttcct tgattgctgt
     4981 tattttttcc tggcccggct ttctttatgc tgtatcgata caccaaagag aaaataatga
     5041 gggagcgaag gatgccagtt ttgcagtggg gaatgttatg tgtgctatca cttctccttt
     5101 ctattggttt cctcgcggta catctcccgg cggcgctatt acttgggccg atgatcgccg
     5161 ggatcatctt cagtatgcgc ggaattactc tgcaacttcc ccgctccgct tttctcgccg
     5221 cgcaggccat tcttggctgc atgattgcgc aaaacctcac tggttcgatt ctcaccacgc
     5281 tggccgccaa ctggccgatc gtgcttgcta ttttactggt gacgctgctt tccagcgcca
     5341 tcgtgggttg gttattggtg cgctatagct cactgcctgg aaataccggt gcctggggtt
     5401 cttctccagg cggcgcagcg gcaatggtcg ctatggcgca agattacggc gcagatattc
     5461 gcctggtggc gtttatgcaa tatctgcgag tgctgttcgt cgcgggggcc gctgttctgg
     5521 tcacccgcat gatgctgggt gataacgctg aagcggttaa ccagcaaatc gtctggttcc
     5581 cgccagtgag cattaatctc ctgcttacga ttttgctcgc agtcgttgcc ggtacggcgg
     5641 gatgcatgct gcgtcttcct tccggcacga tgctcatccc gatgctggcg ggcgcagtgc
     5701 tccagtctgg tcagctcatc accatcgaac tcccggaatg gctgcttgcg atggcgtata
     5761 tggcaattgg ctggcggatt ggtcttggtt tcgataaaca aatcttactg cgggcactac
     5821 gcccgctacc gcaaatcctg ctgtcgattt ttgctctgtt ggctatttgt gcgggtatgg
     5881 cgtgggggct gacccggttt atgcatattg attttatgac tgcctacctc gccaccagcc
     5941 ccggcgggct tgataccgtg gcggtcatcg ccgcagggag caatgccgat atggcgctta
     6001 ttatggcgat gcaaaccctg cgcctgttca gtattttgct gacgggacct gccattgcac
     6061 ggtttatttc aacctatgcg ccgaagcggt cggcttagtg ctggcagcca gggcaccagt
     6121 aaaacgggcg agatgacagc gtggttttct caatgatgct gccacaacgt tcgcacggtt
     6181 cgccatctcg atgaaaaacc ttaaagcgaa acagcgcccc atgatgctta ttctcatcca
     6241 cctgcccccg cgtagcgtaa gaaaatcgag gaatctccag taacgcgtgt gcgagtgcat
     6301 ccagttgcgc cgcattgaga tctttcgctt tatgatttcc agtcaaccca acctgccaga
     6361 ggatctccac ccgcaaataa ttgccaagtc ctgccagaaa cgcctgctcg agcagtaatc
     6421 ctgcaaactg acggttacga aagcgcggcg acaatacccg ttctttcacc acctccggcg
     6481 tcagattcgg atccagcaca tcgggaccaa cgcgttgtaa aaacggatgc gtggtcagtt
     6541 gttccggggt caacatctca atatccgagg cgctataaag cagaatggtt ttgtcagccg
     6601 tttgcagttt tacccgcaat actcgcgtgg tctgcggctc ttcgccggta tcaaccacgc
     6661 gccagacgcc gtaaagctga ttatggctgt agagcgttaa ttcgttggaa aaatgagtta
     6721 acaacgcctt accacgcgtt tccacatggg taacgtgttg accgataagt tgtgattgat
     6781 aagtttttaa ctgcgggaag gcaaaccaga catcagttag tggtttgcct ttgatcgccg
     6841 cctccaggtt atccgctgca cggcggatct ccgggccttc aggcatggtg atatcctttt
     6901 ttcattcttt attaagcggg gttatgctgc gacaacaatc ccctttttgg caaatgtagc
     6961 tcgtaatcgg cgggcgaacg ccagagcgtg ctcgccgtcg ccatgcaggc agaccgtttg
     7021 cgccgcgacc gttgcccatt cgccggtgat acttttgact ctgccgtgtt gcaccatctc
     7081 cagcgtttgc gccagcgcct gttgttcgtt ttcaatcaac gcgcctggct gacttcgcgg
     7141 caccagcgag ccgtcagcct gataaccgcg atcggcaaac acttcctcgc gcgttgtcag
     7201 gccatattgc ttgcctgcac gaatcagctc gcttcctgcc agcccaacga gaatcaatgc
     7261 cggatcgcaa gcgtataccg ctctggcgat ggcgtctgcc agttgtgctt ctttcgccgc
     7321 ctggttgtac aacatgccgt gcggtttgac atgacgcatt acgccgcctt gcgcacgggc
     7381 aatcgttgcc agcgcgccaa tttgatacag cgtctgggcg taaacggttt ctggcggcaa
     7441 ctgcatggcg ctgcgaccaa aattttccct gtcgggaaaa ctcgggtgag cgccaattgc
     7501 aacaccattt tttattgctt cacgcacgca agcctgcatg gtttgcgcat cgcctgcatg
     7561 aaatccacag gcaatattgg cagaggaaac cagcgttaat agctctgcgt cgctggcgca
     7621 gccttcgccc agatcggcgt tcaggtcaat tttcattgtg cagccgccac gctaattgtt
     7681 caaaataacg ttgctgatct tgccgcgctt tcagtgcctc ttccagtgaa cactggacaa
     7741 aatgaatcgg ctgaccgagc ggaatttgcg caagatggta catatcagcc tcaatgatgc
     7801 aggcaatacg cgggtaacca ccggtggtct gtgcgtcgtt catcaacaca atcggctgcc
     7861 cgttatgtgg cacctgcacc acgcccggta acaaaccgtg agataacagt tcgcgatcgg
     7921 tggtgcgttt taaaatttgc ccctgtaagc gatagcccat gcggttactt tgcgagctaa
     7981 gctgccaggg cgaacgccag aatgcatcct gcgaggcgcg atcgaactca tgatattccg
     8041 gccccggcaa ggcgcgaatg cggttgcccc acagcagctg tttaacgccc tgcgcttcca
     8101 taaaatcacg cttcgctttg ccaatcggga gtcggtcacc atccttcagt aaacggcctt
     8161 ccagcccgcc actccccact ttgagatcgg tgctgcatga ccccattacc ggcggaacat
     8221 caataccacc cgcgaccgcc agataactgc gcatcccgtg ctgcgggcgt ttaagcgtta
     8281 aacgctggcc tgctttcatc ggcaatcgcc agccggtcca gacggcatta tcatccagcc
     8341 gcgcttcgca accggcaccc gtcagagcaa accacccatc agtttcgaac tcaacagtta
     8401 actgaccgag cgtgatctcc agcgcggggg cattggcgtc attacccacc agtaggttag
     8461 caatgcgtaa cgcgggcata tccagtgcgc cgcagtggct gatacccgac tggcgaaaac
     8521 cgtgacgacc gccatcctgc acagtggtat acatgcccgc acgaataatc ttcagcatac
     8581 tccctccttc tgcggtacaa agcgcacgct gtctcccgga cgtaataaga tgggttcgtc
     8641 acgcgccgga tcaaacaggc tgagtgaggt atgaccaatc aactgccagc cacccggcgt
     8701 tgccagcgga taaacaccag tctgcggccc gccgatcccg acagaacctg ccggaacgag
     8761 taagcgcggt tcagcgcgcc gtggcgtgtg tagttgttcc ggcaaactcc cgagatacgg
     8821 gaagcccggt tgaaaaccta aaaaccagac cacgtattcc acggaggagt gcaattcaac
     8881 aacctgtttt tcgctcaacc cgcaatgcgc cgcgaccacc gccaaatccg gtccgcctgc
     8941 accaccgtaa accaccggaa tttcaataaa gcgagactca ggttccaggg cctcgctctc
     9001 ctcccaccag cgttgcaaac gctctatggc atccagcgcc agcgactcag gattacgcaa
     9061 aatcaccgta atattgttca tgccgggaat ggcttcaacc acattcggca tatccaccag
     9121 acgctgcgcc agtcgccaga tccgtttctg gctagccagc gtcaccggcg gttccagttc
     9181 cagcactacc gccgtttcac ctatcagata acaacgcgct cgttgcactt tcgatccctc
     9241 tgattattcg ttatgcagga ttaggaatat caataaaggt cacatcaaga tcggtatttt
     9301 cattcagcca ctcgctcaat gcgcgaatac caccacgttc agtggcatgg tgacctgcag
     9361 cataaaaatg caatccctgc tcgcgggctg aatgaatggt ctgttcagaa acttcgccag
     9421 taataaaagc atccacgcca aaacgcgcgg cgctatcgat aaaactttgc ccgccgcccg
     9481 tgcaccaggc gacgcgctgg accacctcag gtccggtatc gccacaccat aacggcttac
     9541 gtcccagacg cgcttcaatc caggaggcta gttccagtcc cggcacaggc atggtcagct
     9601 ctccccacgg caccaacggc tcaatttcgc ccatgacagt gatccccagt aacgccgcca
     9661 gctgtgcgtt attgcccagc tcaggatgtg cgtcaagcgg caaatgccag ccatacaggt
     9721 tgatatcatt cgccagcaac gtttttaaac ggttacgctt cattccgcga atgaccggag
     9781 actctccttt ccagaagtag ccatgatgca caatgaccgc atccgcgccc agacgcactg
     9841 cctcatcgag caaagcctgg ctggcggtga caccggtaac aattttttgc accgtctctt
     9901 tgccttccac ctgcaaacca ttcggcgcgt aatcactaat cgccgcgctg ttcagttttt
     9961 cgttaatcag ttgttccagt tcggtgtttt tcatcttcgc gccttccgta ttctgatagc
    10021 tacaccgtta acatagcgtt acctcgcctg ttcgtcgata gccaatcttt tgacatatta
    10081 agaaacaaac ccgacgccaa aagacgcaaa ttgtttatat catcacgctt cagctaatgt
    10141 atatttatgc aatcagacag catattgcat aaataatcag atctgtatca aagttttaac
    10201 tttaaggaaa cacaagcacg ccgcgcaact attacgtgac gttgccgtca attgtggaat
    10261 tattatgaat aaacacgcat cacaaccgcg cgctatttac tatgtcgttg cgctgcaaat
    10321 ctgggaatat tttagctttt acggcatgcg tgccctgctg attctctatc tcaccaatca
    10381 actaaaatac aacgatactc acgcctacga gttatttagc gcctactgtt cgctggtgta
    10441 tgtcacgcca atcctcggtg gctttttggc ggataaagtt ctcggcaatc gcatggcggt
    10501 gatgctgggg gcgttgttga tggcgatcgg tcatgtggtg ctgggtgcca gtgagatcca
    10561 tccgtcattc ctctatctgt ccctggcgat tatcgtttgt ggctatggtc tgtttaaatc
    10621 caatgtcagt tgcctgctcg gcgagctgta tgagccaacc gatccgcgtc gtgatggcgg
    10681 tttctcgctg atgtatgcag cgggtaacgt ggggtctatt atcgcaccta tcgcctgtgg
    10741 ttacgcccag gaagagtaca gctgggcgat gggctttggc ctggcggcgg tgggtatgat
    10801 cgcgggtctg gtcattttct tatgtggcaa tcgtcatttc acacataccc gcggcgttaa
    10861 taaaaaagta ctgcgtgcaa caaactttct cctgcctaac tggggatggc tgctggttct
    10921 gctggtggca acgcctgcgc tgattaccgt actgttctgg aaagagtggt cggtatacgc
    10981 cttaattgtc gcgactatta ttggcctggg tgtactggca aaaatttatc gcaaagcaga
    11041 aaaccagaaa cagcgtaagg agctggggct gattgtgacg ctcaccttct tcagtatgtt
    11101 gttctgggcc ttcgcacaac agggcggcag ctcgattagc ctttatatcg accgcttcgt
    11161 taaccgcgat atgtttggtt ataccgttcc gactgctatg ttccagtcga ttaatgcctt
    11221 cgcagttatg ctgtgcggtg tgttcctggc gtgggtggtt aaagaaagcg tcgcaggtaa
    11281 tcgtaccgtg cgcatctggg gaaaatttgc tcttggcctt ggcctgatga gcgccggatt
    11341 ctgcattctg accttaagcg cccgctggtc cgcaatgtat ggtcactctt ctctgccact
    11401 gatggtatta ggcctggcgg tgatgggctt tgcggaactg tttatcgacc cggttgccat
    11461 gtcgcaaatt acgcgcattg aaattccggg cgtgaccggc gtattaaccg gcatctacat
    11521 gctgctttct ggcgcgatcg cgaactatct ggcgggcgtg attgccgatc agacatcgca
    11581 ggcttcgttt gatgcttccg gggcgatcaa ctactccatc aatgcatata ttgaagtatt
    11641 tgatcaaatt acctggggcg cactggcgtg tgtaggactg gtactgatga tttggctgta
    11701 tcaggcgctg aaattcagaa accgcgcgct ggcgctggag tcataatagg ccagcaccat
    11761 acctgataaa acgctcgctg cgttttatca ggctcctttc ctcgcttctt cgtacgctgc
    11821 cagcgttcgc agccttgcct ctttgtgatc aactatcggt tgcggataat ccagcatcac
    11881 acctgctttc tgcgcccact tccacggctc atgcaccgct ttccctggta catcgcgcag
    11941 ctccggtaac catcggcgga taaactcacc ttcgcgatca aatttctcgc cctgggttgt
    12001 cgggttgaaa atacgaaaat acggcgctgc atcagtaccc gtagacgccg cccactgcca
    12061 gccaccgtta ttggctgcca aatcaccatc aatcagctgc gacatgaaat atcgctcgcc
    12121 ttcgcgccag tcgatcaaca aatctttcac cagaaaactg gctgaaatca tccgtagccg
    12181 gttatgcatc cagccagtgc tgttaagctg acgcatggcg gcatcgacaa tcgggtatcc
    12241 cgttttgcct tcctgccagg cctgtaaatg tgcgggattg ctctgccact gcacacgatc
    12301 cgtccaggca ataaacggac aatgtttaca caacgagggg taatacgtca tcagatgacg
    12361 gtagaactcg cgccagatca gctcactaag ccagacacta ccggccccac cgtccagcgc
    12421 ctgcggctgt tccgccaaca agcgatgcaa acactggcga ggcgataacc cgcccgttgc
    12481 caggctggcg gacaaacgac tggtgccttc cactgccgga aaatctcgtt gttgctcata
    12541 ttctccggca ccgttctggc aaaactggcg taattgcgca atcgccgctt tttcttccac
    12601 cgggaaatgc gcagtatcga aagactgacg aggataattc agcgtaatgg atggcgaggg
    12661 ctctatcgat ccgctactac gaacttttgg cgcagcgacg cactccggca tcccttcccg
    12721 cagccgtttc agccaggcat tcttaaaagg cgtaaagact ttgtacatct cgtgattacc
    12781 ggtcttcacc gcgccaggcg gcaggatcac gctgtcatca aatccttcac acaccacgtt
    12841 acgcagcgct ctttcaactt ccacatcccg cgcccgctca ttcacttcat actgatagtt
    12901 ataaaacagg tgggtaacgc tgttttccgc gcacacctgt ttaactattt cgacactggc
    12961 gacaaagtca tccacttcac ggaacaataa aggaatacct ttttccgcaa gcgctatttg
    13021 tagcccattc agttgagcat tgataagttc agcctgacgc ggcgacatgt tatgcgtcgc
    13081 ccactggcgt ggtgtagcga tatacaacgc cagcacgcgt gcagacgaat tgcggcaggc
    13141 ggcagccagt gcgagattat cgtgcagacg taaatcctgg cgaaaccaga ccagatgggt
    13201 agtcataaaa ctcctgaatg gcgcaagtta atcacacgcg gccaaagttc gaaataacgc
    13261 tgccctgaaa gccaggcgtc aggataaagc gccatatagt gtttgatacg catcagcggc
    13321 gcaagaagtg gttgctcgcc acggcgataa ctgtcgataa gcgccgccaa ctgctggcgt
    13381 tctgtggaat caatatgcgg gcgaaaataa ccctgaacgt gcatcaaaac gttggtgtga
    13441 tcgcgcgggt tggcgggatg agaaagcaaa acaattacgc gttggtagta ctggttgtaa
    13501 aaatcatcca gattttgcca ctggtgaata tcagccacga acgggccaag tttgcggtac
    13561 tccggctgag aatgcgccag aaagacgagt ttatagcggc tgtgataatc gagaagcgcc
    13621 ccgcgcgtta agccatgttc tttcagtcga tgtaactcat gaagggcgaa gatacggatt
    13681 agggtgctgt catcaaatcg ttgctgattc attactgctc ctgaacggtt tacaggagta
    13741 agtgtagacg ttgagaaatg atatttgtga agttaatcct gttgtagcag gacaaattta
    13801 cgaaagcctg ctccccgcaa ggactgacgt caggtagttt ctaaccatag ctgccctttc
    13861 tattcaaacg tgaaatcaat aagctcatca gggacatcgc aaacaatatg aagacgggtt
    13921 tcttctctgc cgtgactctt ttcactaatt gcgtaactgt catgcttggg attatttaat
    13981 tctttcagcg gaaatttttc ctcgaaggct ttatttagcc gcccctggtt tccttttaca
    14041 gcgaataaat aatcacctcc ctgtttttgt atcttctctg caatatcttt ctggcaaccc
    14101 atcgcatcgg ttgtgatgat ttttccttta atatccagca tgttaagaag ttctgggata
    14161 gctgtgatct cattggattt cttatccgtc ttgatctgcc cgatgaccag actgtgcatt
    14221 gttgagaacg cactaatgac atgaatcgct cccctgcggc gactcttgtc ataagagtgc
    14281 cggagcgttt ttccatcaat tgcaatgacg tctttatcat ttgatgaatg gcaatcacgc
    14341 atccagttaa taaagcactc gtgaaatttt gcaggactga tacaggatac aactctggca
    14401 atagtatcgt gaacaggaat a
//
LOCUS       DAZFWL020000120        14281 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFWL020000120 DAZFWL020000000
VERSION     DAZFWL020000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.120"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            200..1771
                     /gene="garD"
                     /locus_tag="ACHKNP_RS18950"
                     /old_locus_tag="pgaptmp_003790"
     CDS             200..1771
                     /gene="garD"
                     /locus_tag="ACHKNP_RS18950"
                     /old_locus_tag="pgaptmp_003790"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HGU4705880.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEMIRYGEVIGYAVRAIPRGSWLDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDIASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1920..2255
                     /gene="prlF"
                     /locus_tag="ACHKNP_RS18955"
                     /old_locus_tag="pgaptmp_003791"
     CDS             1920..2255
                     /gene="prlF"
                     /locus_tag="ACHKNP_RS18955"
                     /old_locus_tag="pgaptmp_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HGU4705881.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2255..2719
                     /gene="yhaV"
                     /locus_tag="ACHKNP_RS18960"
                     /old_locus_tag="pgaptmp_003792"
     CDS             2255..2719
                     /gene="yhaV"
                     /locus_tag="ACHKNP_RS18960"
                     /old_locus_tag="pgaptmp_003792"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HGU4705882.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(2774..3583)
                     /gene="agaR"
                     /locus_tag="ACHKNP_RS18965"
                     /old_locus_tag="pgaptmp_003793"
     CDS             complement(2774..3583)
                     /gene="agaR"
                     /locus_tag="ACHKNP_RS18965"
                     /old_locus_tag="pgaptmp_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HGU4705883.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVVERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3832..5112
                     /gene="kbaZ"
                     /locus_tag="ACHKNP_RS18970"
                     /old_locus_tag="pgaptmp_003794"
     CDS             3832..5112
                     /gene="kbaZ"
                     /locus_tag="ACHKNP_RS18970"
                     /old_locus_tag="pgaptmp_003794"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HGU4705884.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCHDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5135..5608
                     /gene="agaV"
                     /locus_tag="ACHKNP_RS18975"
                     /old_locus_tag="pgaptmp_003795"
     CDS             5135..5608
                     /gene="agaV"
                     /locus_tag="ACHKNP_RS18975"
                     /old_locus_tag="pgaptmp_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HGU4705885.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5619..6398
                     /gene="agaW"
                     /locus_tag="ACHKNP_RS18980"
                     /old_locus_tag="pgaptmp_003796"
     CDS             5619..6398
                     /gene="agaW"
                     /locus_tag="ACHKNP_RS18980"
                     /old_locus_tag="pgaptmp_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HGU4705886.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6388..7266
                     /gene="agaE"
                     /locus_tag="ACHKNP_RS18985"
                     /old_locus_tag="pgaptmp_003797"
     CDS             6388..7266
                     /gene="agaE"
                     /locus_tag="ACHKNP_RS18985"
                     /old_locus_tag="pgaptmp_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HGU4705887.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7284..7718
                     /gene="agaF"
                     /locus_tag="ACHKNP_RS18990"
                     /old_locus_tag="pgaptmp_003798"
     CDS             7284..7718
                     /gene="agaF"
                     /locus_tag="ACHKNP_RS18990"
                     /old_locus_tag="pgaptmp_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HGU4705888.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLALQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7715..8848
                     /gene="nagA"
                     /locus_tag="ACHKNP_RS18995"
                     /old_locus_tag="pgaptmp_003799"
     CDS             7715..8848
                     /gene="nagA"
                     /locus_tag="ACHKNP_RS18995"
                     /old_locus_tag="pgaptmp_003799"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU4705889.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            9199..10353
                     /locus_tag="ACHKNP_RS19000"
                     /old_locus_tag="pgaptmp_003800"
     CDS             9199..10353
                     /locus_tag="ACHKNP_RS19000"
                     /old_locus_tag="pgaptmp_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HGU4705890.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10366..11226
                     /gene="kbaY"
                     /locus_tag="ACHKNP_RS19005"
                     /old_locus_tag="pgaptmp_003801"
     CDS             10366..11226
                     /gene="kbaY"
                     /locus_tag="ACHKNP_RS19005"
                     /old_locus_tag="pgaptmp_003801"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HGU4705891.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11393..11869
                     /gene="agaB"
                     /locus_tag="ACHKNP_RS19010"
                     /old_locus_tag="pgaptmp_003802"
     CDS             11393..11869
                     /gene="agaB"
                     /locus_tag="ACHKNP_RS19010"
                     /old_locus_tag="pgaptmp_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HGU4705892.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11908..12711
                     /gene="agaC"
                     /locus_tag="ACHKNP_RS19015"
                     /old_locus_tag="pgaptmp_003803"
     CDS             11908..12711
                     /gene="agaC"
                     /locus_tag="ACHKNP_RS19015"
                     /old_locus_tag="pgaptmp_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HGU4705893.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12701..13492
                     /gene="agaD"
                     /locus_tag="ACHKNP_RS19020"
                     /old_locus_tag="pgaptmp_003804"
     CDS             12701..13492
                     /gene="agaD"
                     /locus_tag="ACHKNP_RS19020"
                     /old_locus_tag="pgaptmp_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HGU4705894.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13493..14248
                     /locus_tag="ACHKNP_RS19025"
                     /old_locus_tag="pgaptmp_003805"
     CDS             13493..14248
                     /locus_tag="ACHKNP_RS19025"
                     /old_locus_tag="pgaptmp_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HGU4705895.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQLLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         3452 a         3622 c         3721 g         3486 t
ORIGIN      
        1 tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc gggcggcact
       61 gcaatccctg aaatgattga cattgatcac atttctgcgt ttaaactcct gacattctta
      121 tttcacccaa tgaagtcatt tatttttaaa tgagaccagg tcctcatttt aataacccct
      181 ggctggagaa tatagtacaa tggccaacat cgaaatcaga caagaaacgc caactgcgtt
      241 ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata atggcctgaa
      301 agcaggaacg cgttttccgg atggactgga attaattgaa catattcccc aggggcataa
      361 agtcgcattg ctggacattc cggctaatgg tgaaatgatt cgttatggtg aagtgattgg
      421 ttacgccgtg cgtgcaatcc cacgcggaag ctggctcgac gaatcaatgg ttgtactacc
      481 ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggccc ccttaccgcc
      541 gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg gcaccaaaaa
      601 cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact atgtagtaaa
      661 aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg tggggctgaa
      721 tcatttgtac ggctgtggcg tggcgattaa cgcaccggcg gcagttgtgc ctattcgtac
      781 catccacaat atttcgctga accctaactt tggcggcgaa gtaatggtga ttggtctggg
      841 ttgtgaaaag ttgcagcctg agcgcctgct ggtcggcacg gatgatgtgc aagctattcc
      901 agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt ttcagtccat
      961 ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc aacgtcagcg
     1021 agaaacctgt ccggcttcag aactggttgt cggcatgcag tgcggtggca gcgatgcctt
     1081 ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg tgcgctgcgg
     1141 cgcaacggtg atgttctcag aagtcacgga agtgcgtgac gcgatccatc tgctgacacc
     1201 acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt ggtacgataa
     1261 ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca acaagaaagg
     1321 cggtctggca aacgtggtag agaaggcact cggctccatt gctaaatcgg gtaaaagcgc
     1381 aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa tttacgccgc
     1441 gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta tcaccgtgca
     1501 agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg tcattaaaat
     1561 ggcgacccgc accgagctgg cgaaccgctg gtttgattta atggatatta acgcgggcac
     1621 tatcgccacc ggcgaagaaa ccattgaaga ggtgggctgg aagttgttcc actttattct
     1681 cgacatcgcc agcgggaaga agaaaacctt ctcggatcaa tgggggctgc ataaccagct
     1741 ggcagtgttt aacccggcac cggtgacctg atttcttttg gaacgtccct cgcaaaacat
     1801 ggccttagtg ccatgttttt attgtttaaa gcccccacgt ccattaataa tgcatttgca
     1861 ttaccttagt tcaagcttat aatttgagca gaaaacaggc tgtaaaagga cagtgaatca
     1921 tgcccgctaa tgctcgctct cacgctgtac tgaccactga atcaaaggtc acgatacgcg
     1981 gacaaacaac tatccccgca ccagtgcgtg aggccttaaa actgaagcca ggccaggaca
     2041 gcattcatta cgaaattctg cctggtgggc aagtctttat gtgccgactg ggagatgaac
     2101 aggaggatca tactatgaat gcatttttgc gttttctgga tgcagatatc cagaacaacc
     2161 cgcaaaaaac tcgtccattc aacattcaac aaggaaagaa acttgtcgct ggcatggacg
     2221 tcaacattga tgatgagatt ggcgacgacg aataatggat tttccacaaa gggttaatgg
     2281 ttgggcgcta tatgctcatc cctgttttca ggaaacctac gacgctttag ttgccgaagt
     2341 cgagacatta aagggaaaag atcctgaaaa ttatcagaga aaagccgcca caaagttatt
     2401 ggcggtagtc cataaagtga ttgaggagca tatcacggtc aatccatcat caccggcatt
     2461 ccgtcatggc aagtcgttag gctctgggaa aaataaagac tggtcacggg taaaatttgg
     2521 tgctggtcgt tatcgtctct tctttcgtta tagtgaaaaa gagaaagtca tcattctggg
     2581 atggatgaac gatgaaaaca ctctgcgcac ctacggtaaa aaaacagatg cctataccgt
     2641 attcagcaaa atgttaaaaa gaggacatcc tcctgccgac tgggaaaccc tcacccgaga
     2701 aacagaagaa acccattgat ggtgttcaca atgcccgccg aacgtattcg tgttgaccag
     2761 cgggcaccct ttctcactcc ccgaccagaa tcacttcaac cccagccttt cgcagtcctt
     2821 ccagactatc cgcaggaatg ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa
     2881 tgatcttatg taaactggaa cgattgaatt tactggaatc ggtgaccacg atgatccgtt
     2941 ccacaacttc gcacatccga cggtttaaac gggcttcatc ttcattatgt gtgctgacgc
     3001 cgcgctccag atcgatcgca tctacaccaa gaaacagcat atcgaagtgg taattttgca
     3061 gcgattgctc agcctgatcg ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg
     3121 tcatcagcag ctcaacgcct tccgcttcca gcaacgcatt agccacgttc ataccgttgg
     3181 tcatcgcaat tacgtcagtg tgcttacgca tcagacgagc aatctcaaaa gtggtagtcc
     3241 cggaatcgag gatcacccga tgaccaggct gaatcaactc aacggcagct ttcgcaacgc
     3301 tgcgtttcat cgcggtgttc agcgcgcttt tatcttccac tgatggctcg actgacggcg
     3361 tcgtgctatc gcagatcaac gcgccaccat aggcacgcac agcgatcccc tgcttttcca
     3421 gaaacgccag atcattgcgg atcgtcacag tagatacgcc atacaatgcc gacagatcgt
     3481 taacctgcac actcccttgc tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg
     3541 tgccagtcac tcgcttctca cctgaagcgt cggtattact catagtaagt cctttcgtaa
     3601 aactttcgtt tcatttcgtt ttgcctatta acgcctttct attaagcaaa tgcaagccca
     3661 ccttgcccat tgacgcaagc tactctcgtt tcagtgactt tcattatgtt tcttttgtga
     3721 atcagatcag aaaactatta tctttcgttt tatttttatc tcaccatgac gcagtatcaa
     3781 ctgaaacaaa acgaaagatt aatatcgcag taatctgaac tggagaggaa agtgaaacat
     3841 ctgacagaaa tggtgagaca gcacaaagcg ggcaaaacaa atggaattta tgccgtttgt
     3901 tccgcacatc cgctggtgct ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg
     3961 ttactgattg aagcaacctc caatcaggta gaccagttcg gcggttatac cggaatgacg
     4021 cccgccgatt ttcgcggctt tgtttgtcag ctcgccgact cgttgaattt cccgcaggat
     4081 gcgttgattc tgggtggtga ccatctgggg ccaaaccgct ggcaaaacct gccggccgct
     4141 caggcaatgg ccaatgccga tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa
     4201 atccaccttg attgcagcat gtcctgtcat gacgatccga ttcccttaac tgatgacatc
     4261 gtggctgaac gcgccgcccg tctggcgaaa gtggcggaag aaacctgtct tgaacacttt
     4321 ggcgaagccg atctggagta tgtcattggt accgaagtgc cggtacctgg cggcgcgcat
     4381 gaaaccttaa gcgagctggc ggtcaccacg ccggatgccg cccgcgccac gctggaagcc
     4441 catcgtcacg cctttgaaaa gcaaggtttg aatgccatct ggccacgcat cattgccctg
     4501 gtggttcaac ccggcgtcga attcgatcac accaacgtta ttgattatca gcccgccaaa
     4561 gcgagcgcct taagccagat ggtcgaaaac tacgaaacgc tgattttcga agcgcactct
     4621 accgattatc aaacgccgca atcgctgcgc cagctggtga ttgaccactt tgccattctg
     4681 aaagttggcc cagcgctgac cttcgccctg cgtgaagctc tgttctctct ggcggcgatt
     4741 gaagaagaac tggtgccagc gaaagcctgt tctggtctgc gtcaggtgct ggaagacgtg
     4801 atgctcgacc gcccggaata ctggcaaagc cactaccacg gtgacggcaa cgcgcgtcgt
     4861 ctggcgcgtg gttatagcta ctcggatcgc gtgcgctatt actggccgga cagccagatt
     4921 gatgacgctt tcgctcatct ggttcgtaat ctggcggatt caccaattcc gctaccgctc
     4981 atcagccagt atctgccgct gcaatacgtg aaagttcgct ccggcgagct gcaaccgacg
     5041 ccacgggaac tcattatcaa ccatattcag gacatcctgg cgcagtacca cacagcctgt
     5101 gaaggccaat aagcaaaaca aagaggaaca cgctatgcca aatattgttt taagccggat
     5161 tgatgaacgc ttgattcacg gtcaggtcgg cgttcagtgg gtcggatttg cgggggcaaa
     5221 tctggtgctg gtagccaacg atgaggttgc cgaagatccg gtacaacaaa acctgatgga
     5281 aatggtactg gcagaaggga tcgccgtacg tttctggacg ctgcaaaaag ttatcgacaa
     5341 cattcatcgc gccgccgatc gacagaaaat cctgctggtt tgtaaaacac ccgccgattt
     5401 cctgacgctg gtgaaaggtg gcgttccggt gaatcgcatt aacgttggca atatgcacta
     5461 cgccaatggc aaacaacaaa tcgccaaaac ggtttctgtg gatgcgggcg atatcgcagc
     5521 atttaacgac ctgaaagccg ctggggtgga atgcttcgtt cagggcgtcc cgacagagcc
     5581 tgctgtagac ctctttaaat tactttgagg gattcatcat ggaaatcagc ctgttgcagg
     5641 catttgcgtt gggcattatc gcctttatcg ctggcctgga tatgtttaac ggcctaacac
     5701 atatgcaccg cccggtggtc ctcggcccgt tggtcgggct ggtacttggc gatctgcata
     5761 ccggaatttt aaccggcggt acgctggaac tggtgtggat ggggctggcc ccgctggcgg
     5821 gcgcacagcc gcctaacgtg attatcggta ctatcgtcgg cacggcgttt gccattacta
     5881 ctggcgtgaa acccgatgtc gcggtcggtg tcgccgtacc tttcgctgtc gcagtacaga
     5941 tggggattac cttcctgttc tcggtgatgt ctggcgtgat gtctcgctgc gaccggatgg
     6001 cggagaacgc cgatactcgc ggcattgaac gcgtgaacta tctggcactg ctggcactcg
     6061 gcactttcta ttttctctgc gctttcctgc ctatctactt cggcgcggaa catgcaaaaa
     6121 ccatcattga tgtcctgccg caacgattaa tcgacggcct cggcgtcgcg ggcggcatca
     6181 tgccagcaat cggttttgcc gtgctgctga aaatcatgat gaaaaacgtc tacatcccct
     6241 acttcatcct gggctttgtt gccgcagcct ggctcaagtt accggtgctg gctattgctg
     6301 ccgctgcgct ggcgatggcg ctgatcgacc tgctgcgtaa atctcctgaa ccgactcaac
     6361 ctgcggcaca gaaagaggaa tttgaagatg gcatctaatc aaaccaccct gccgaacgtc
     6421 agtgaaaacg aagaaacatt gctgactggc gtcaatgaaa acgtgtatga agatcagagc
     6481 attggcgcgg agctgacgaa aaaagatatc aaccgcgtcg cctggcgttc catgctgtta
     6541 caagcgtctt ttaactacga acgtatgcag gcttctggct ggttgtacgg tctgctgcct
     6601 gctctgaaaa agatccacac taataaacgc gacctggcgc gcgccatgaa ggggcatatg
     6661 ggtttcttca atacccatcc gtttctggtg acatttgtta tcggcattat ccttgcgatg
     6721 gagcgttcta agcaggacgt taacagtatt cagagcacca aaattgccgt cggtgcgccg
     6781 ctcggcggga ttggcgatgc aatgttctgg ctaacgctac tgccgatttg tggcgggatt
     6841 ggagccagtc tggcattgca aggctccatt cttggcgcag tcgtctttat tctgctgttc
     6901 aacgtggtac acctggggct gcgttttggt ctggcgcatt atgcttaccg catgggcgtg
     6961 gcggcgattc cactcattaa agcgaatacc aaaaaagtcg gccatgctgc gtctatcgtt
     7021 gggatgacgg taatcggtgc gctggtggca acctatgttc gtttaagcac cacgctggaa
     7081 atcacagcgg gcgacgcagt ggttaagtta caggctgatg ttatcgacaa actgatgcct
     7141 gccttcttac cgttggtcta caccctgacc atgttttggt tggtacgccg cggctggagt
     7201 ccgctgcgtc tgattgcagt taccgtggtt ctcggcatcg tcggtaaatt ctgccatttc
     7261 ctttaaatac aatgaggtat tagatgttaa gtattatttt gacagggcat ggcgggtttg
     7321 ccagcggcat ggaaaaagca atgaagcaaa ttctcggtga gcaatcgcag tttatcgcca
     7381 tcgattttcc ggaaacctca agcaccgcgc tgcttacatc gcagcttgaa gaggccatcg
     7441 cccaactgga ttgtgaagac ggcattgttt ttctgacgga tttgctgggc ggcacgccgt
     7501 ttcgcgtagc ttcaacgctg gcgctgcaaa aaccgggctg tgaagtgatc accggcacca
     7561 atttgcaact gctactggag atggtgctgg agcgcgaagg gttaagcggt gaagaattcc
     7621 gtgtacaggc gctggagtgt ggacatcgtg ggctgaccag tctggtggac gagttaggcc
     7681 gctgccatga agaatgtccg gtcgaggaag ggatatgaca cacgttctgc gcgccagaag
     7741 gctgctgact gaagagggat ggctcgatga ccatcagttg cgtattgctg acggtgtcat
     7801 cgcagcaatc gaaccgattc cagtgggcgt gactgaacgc gatgcggaac tgctctgccc
     7861 cgcttacatc gacacccatg tacacggtgg tgcgggcgtt gatgtaatgg atgacgcgcc
     7921 ggatgtactc gacaagctgg caatgcacaa ggcacgcgaa ggtgtcggca gttggttgcc
     7981 gactaccgta accgcgccgc ttaacaccat tcatgcggcg ctgaaacgta ttgctcaacg
     8041 ttgccaacgc ggcggacctg gtgcgcaagt gctggggagt tatctcgaag gaccgtactt
     8101 cacgccgcag aataaaggcg cgcatccgcc ggaattgttt cgcgagcttg aaattgccga
     8161 gctggatcaa ttgattgccg tttctcagca caccttacgc gtggtagcgc tggcaccgga
     8221 aaaagagggg gcattgcagg ccatccgcca tcttaaacag caaaatgtac gagtgatgct
     8281 ggggcatagc gcggcgacct ggcaacaaac tcgcgccgcg tttgatgctg gtgccgacgg
     8341 cctggtgcat tgctataacg ggatgacagg tttacatcac cgcgaaccgg gaatggttgg
     8401 cgcgggatta acggacaagc gcgcctggct ggaactgata gccgatggtc atcatgtgca
     8461 tccggcggcg atgtcgctgt gttgttgctg tgcaaaagag agaatcgtgc tgatcaccga
     8521 cgcgatgcag gcagccggga tgccggatgg tcgctatacg ttatgtggcg aagaagtgca
     8581 gatgcacggt ggcgttgtcc gtaccgcgtc cggtgggctg gcgggcagta cgctgtctgt
     8641 tgatgcggca gtgcgcaaca tggtcgagtt gacgggcgta acgcctgcgg aagccattca
     8701 tatggcatcg ctgcatccgg cgcgaatgct gggtgttgat ggtgttctgg gatcgcttaa
     8761 accgggcaaa cgcgccagca tcgttgcgct ggatagcggg ctgcatgtgc aacaaatctg
     8821 gattcagagt caattagctt cgttttgata ttttgctcct ttattgggcc ttcacttccc
     8881 ccgtaaggcc tttctttttc tttcgttttg atctgtgcag cggtgtcgga tgcgacgcta
     8941 acgcgtctta tgcgacctac ggtacgccgc atccagcaat cggtgaacat agcgacgtca
     9001 ttacgcaatt ctttaatctc cccttgtctt ttctttcaat gatcacagat ttcgttttat
     9061 ttcctttccc tccattgaac tttcggtttc ttttctatag attttaatca acgaaagaca
     9121 tcaccaagtg aaatgaaacg aaaggcaagt gaaagcgaca acgcccgacg tcaagttcat
     9181 cagactaagg attgagttat gccagaaaat tacacccctg ctgccgccgc aaccggtaca
     9241 tggactgaag aagagatccg ccatcagcct cgcgcatgga tccgttcact caccaacatc
     9301 gacgcgctac gttctgcgct caataacttc cttgaaccgt tactgcgcaa agagaatctg
     9361 cggatcatcc tgaccggagc cggaacctcg gcatttatcg gtgacatcat cgcgccgtgg
     9421 ctcgccagcc ataccggtaa aaacttcagc gccgtaccga ccaccgatct ggtcaccaat
     9481 ccgatggact acctgaaccc agctcatccg ctgctgttga tctccttcgg tcgatccggc
     9541 aacagcccgg aaagcgtcgc tgccgtggaa ctggcaaatc aatttgtacc agaatgctat
     9601 cacctgccga tcacctgcaa cgaagcgggc gctctttacc aaaacgcgat caacagcgat
     9661 aacgcgtttg ccctgctgat gcccgcagaa acgcacgatc gcggctttgc gatgaccagc
     9721 agcattacca ccatgatggc cagctgcctc gcggttttcg cacctgagac gatcaacagc
     9781 caaaccttcc gcgacgtggc ggatcgttgc caggcgatcc tgacctcact gggcgatttc
     9841 agcgaaggtg tgtttggtta cgcaccgtgg aaacggatcg tttatctcgg cagcggtggc
     9901 ttacagggcg cagcacgcga gtcggcgctg aaagtgctgg aactgactgc gggtaaactg
     9961 gcggccttct atgattcccc gaccggattc cgtcatggcc cgaaatcact ggtcgataac
    10021 gaaacgctgg tggtggtatt tgtctcaagc cacccttaca cccgtcagta tgatcttgat
    10081 ctgctggctg aactccgccg tgacaaccag gcaatgcgcg taatcgccat cgccgcggaa
    10141 agcaccgaca tcgtcgctgc cggtccacat attatcctgc cgccgtcacg tcactttatc
    10201 gacgttgagc aggcattttg cttcctgatg tacgcccaga cgtttgcact gatgcagtcg
    10261 ctgcacatgg gcaatacgcc ggatacccca tcagccagcg gcaccgttaa ccgcgtggtg
    10321 caaggcgtaa tcattcatcc gtggcaggca taagaggatc gcattatgag cattatctcc
    10381 actaaatatc tgttacagga cgcccaggcc aatggctacg cggtgcctgc ttttaacatt
    10441 cataacgccg agacgatcca ggcgatcctc gaagtgtgca gtgaaatgcg atcgccggtg
    10501 atcctcgccg gaacgccggg gacctttaaa catatcgcgc tggaagagat ctacgccctg
    10561 tgcagcgcct attccacaac ctacaacatg ccactggcgc tgcatctcga ccaccacgaa
    10621 tcgctggatg atattcgccg taaagtccac gcaggtgtgc gcagtgcaat gatcgacggc
    10681 agccacttcc cgtttgccga gaacgtgaag ctggtgaaat cggttgttga cttctgccac
    10741 tcacaggatt gcagcgtgga agcagaactg ggccgcctgg gcggtgttga agatgacatg
    10801 agcgttgacg ccgaaagtgc attcctgacc gatccacaag aagctaaacg ctttgtcgaa
    10861 ctgactggcg tcgacagcct ggcggtagcg attggtacgg cgcacggctt atacagcaaa
    10921 acgccgaaga ttgatttcca gcggctggcg gaaattcgtg aagtggtgga tgttcctctg
    10981 gtgctacatg gtgccagcga tgttccggat gaatttgtcc gtcgcactat tgaacttggc
    11041 gtcacaaaag tgaacgttgc cacagaatta aaaatagcct tcgctggcgc ggttaaagcc
    11101 tggtttgcgg aaaatccgca gggtaatgat cctcgttatt atatgcgcgt cggaatggat
    11161 gcgatgaaag aagttgtcag aaataaaatt aatgtctgtg gttcagcgaa tcgaatttca
    11221 gcataatcat tagtttttta tgatttatcc caatgtactt cccgattaat atcggggagt
    11281 gccttaatgg aaaaggagat gactaaacct taataaatac atcagtacaa tatcgcaaca
    11341 ataatatatt aaaaaaaatt atattattca actttatggt gaggattaca caatgagcag
    11401 tccaaatatt ctcttaaccc gtattgataa ccgtctggtt cacggtcagg ttggcgtgac
    11461 ctggacatcc accatcggtg cgaatctgct ggtagtcgtg gatgatgttg tcgctaacga
    11521 tgatattcaa cagaaattaa tgggtattac cgcagaaacc tacggctttg gcattcgttt
    11581 ctttactatc gaaaaaacca ttaacgtcat cggcaaagct gcaccgcatc agaagatctt
    11641 cctgatttgc cgtacgccac aaacggtacg taaattggta gaaggtggca ttgacctgaa
    11701 agatatcaac gtcggcaata tgcatttctc ggaagggaaa aagcaaatca gcagtaaagt
    11761 ttatgtcgat gaccaggatc tcgcggactt acgttttatt aaacaacgtg gcgtgaatgt
    11821 tttcattcag gacgtccctg gcgatcaaaa agaacaaatc cctgactaaa tctaaaatcg
    11881 ccttaatatt ggtttgaggt aataaaaatg catgaaataa ccctacttca gggattatcc
    11941 ctggcggcgt tagttttcgt tctggggatt gatttttggc tggaagcctt atttttattc
    12001 cgcccgataa tcgtttgtac cctaactggc gctattctcg gtgatattca gactggctta
    12061 attaccggtg gtctgacaga gttggctttc gccggattaa cccctgcagg tggtgttcag
    12121 ccgcccaacc cgattatggc gggtctgatg accaccgtca ttgcatggtc tacgggcgtt
    12181 gatgccaaaa cagcaattgg tcttggcctg ccgtttagtt tgttaatgca gtacgtcatt
    12241 ctgttcttct attccgcttt ctcattattt atgaccaaag ccgataaatg cgcgaaagag
    12301 gcggatacgg cagcgttttc ccggcttaac tggacaacga tgctcatcgt cgcttcagcg
    12361 tatgcggtga ttgctttcct ctgtacttac ctggcacaag gggcgatgca ggcgctggtg
    12421 aaagcgatgc ccgcctggtt gacccacggc tttgaagtgg ctggcggtat tctgcctgcc
    12481 gttggttttg gcttgctgct gcgcgtaatg ttcaaagcgc aatatatccc ttacctgatc
    12541 gccggtttcc tgtttgtttg ctacatccag gtcagcaacc tgttgccggt tgccgtactg
    12601 ggcgcaggct ttgcggtgta tgagtttttc aatgcgaaat cccggcagca agcgcaaccg
    12661 cagcccgttg ccagtaaaaa tgaagaagag gactacagca atgggatctg aaatcagtaa
    12721 aaaagatatc acccgtctgg gctttcgttc gtcgctgctg caagcgagct ttaactacga
    12781 aaggatgcag gcgggcggtt ttacctgggc gatgttgccg atcctgaaaa agatttataa
    12841 ggacgacaaa ccgggcttaa gcgcggcgat gaaagataac ctcgaattta ttaataccca
    12901 cccgaatctg gtcggattcc tgatggggtt attaatttcg atggaagaaa aaggagaaaa
    12961 ccgcgacacc attaaaggcc tcaaagtggc actgtttggc ccaatcgccg ggattggcga
    13021 tgcgattttc tggtttactt tgttgccgat tatggcggga atttgctcat catttgccag
    13081 ccagggaaac ctgctggggc cgattctatt tttcgccgtt tacctgctta tctttttcct
    13141 gcgcgtcggc tggacccacg tcggttattc agtcggcgtg aaggcgatcg ataaagtgcg
    13201 agagaactcg cagatgattg ctcgctcggc aaccatcctc gggatcacgg taatcggcgg
    13261 gctgatcgct tcgtatgtgc atattaacgt ggtgacatcg tttgccatcg acagtaccca
    13321 cagcgtcgcg ctgcaacagg atttcttcga taaagtcttc ccgaacattt taccgatggc
    13381 ctacaccctg ctgatgtatt acttcctgcg ggtgaaaaaa gcgcatccgg tgctgttaat
    13441 cggcgtgact tttgtgctct ctattgtttg ttccgcattc ggcattttgt aaatggaacg
    13501 aggcactgct tctggcggtg cctctttact taaggaattt catcctgtgc aaacccttca
    13561 gcaagttgaa aactatacgg cgttaagtga acgagccagc gaatatttat tggctgtgat
    13621 ccgtagcaaa ccggatgccg tgatttgcct ggcgaccgga gccacgccat tactgacgta
    13681 tcattatctg gtagaaaaaa tccaccagca gcaggttgat atcagccagc tcaccttcgt
    13741 gaagctcgac gaatgggtgg atctgccatt aacgatgcct ggcacctgcg aaactttcct
    13801 acaacagcat atcgtgcagc tgctggggct gcgtgaagac cagctcatca gctttcgctc
    13861 cgaagagata aatgagacag agtgcgaacg ggtaacgaac ctgattgcgc gcaaaggcgg
    13921 tctggattta tgcgttctag gattggggaa aaacggtcat cttgggctga acgaaccggg
    13981 agaaagccta caaccggcct gccatatcag tcaacttgat gccagaacac agcaacatga
    14041 gatgttaaaa accacgggtc gccccgtgac tcgtgggatc accttaggcc tgaaggatat
    14101 tctcaatgcc cgcgaggttt tgttattggt gactggcgaa ggaaagcagg atgcgacaga
    14161 acgttttctc acggctaaag tctccaccgc tatcccggct tcgtttttat ggctgcacag
    14221 taactttatt tgtttaatta atacctaacc agatgaaaat ttgctcaaaa tttttaaatt
    14281 a
//
LOCUS       DAZFWL020000121        14237 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFWL020000121 DAZFWL020000000
VERSION     DAZFWL020000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.121"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(88..168)
                     /gene="shoB"
                     /locus_tag="ACHKNP_RS19030"
                     /old_locus_tag="pgaptmp_003806"
     CDS             complement(88..168)
                     /gene="shoB"
                     /locus_tag="ACHKNP_RS19030"
                     /old_locus_tag="pgaptmp_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HGU4705896.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(588..968)
                     /gene="acpS"
                     /locus_tag="ACHKNP_RS19035"
                     /old_locus_tag="pgaptmp_003807"
     CDS             complement(588..968)
                     /gene="acpS"
                     /locus_tag="ACHKNP_RS19035"
                     /old_locus_tag="pgaptmp_003807"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGU4705897.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(968..1699)
                     /gene="pdxJ"
                     /locus_tag="ACHKNP_RS19040"
                     /old_locus_tag="pgaptmp_003808"
     CDS             complement(968..1699)
                     /gene="pdxJ"
                     /locus_tag="ACHKNP_RS19040"
                     /old_locus_tag="pgaptmp_003808"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HGU4705898.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1711..2439)
                     /gene="recO"
                     /locus_tag="ACHKNP_RS19045"
                     /old_locus_tag="pgaptmp_003809"
     CDS             complement(1711..2439)
                     /gene="recO"
                     /locus_tag="ACHKNP_RS19045"
                     /old_locus_tag="pgaptmp_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGU4705899.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2451..3356)
                     /gene="era"
                     /locus_tag="ACHKNP_RS19050"
                     /old_locus_tag="pgaptmp_003810"
     CDS             complement(2451..3356)
                     /gene="era"
                     /locus_tag="ACHKNP_RS19050"
                     /old_locus_tag="pgaptmp_003810"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGU4705900.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3353..4033)
                     /gene="rnc"
                     /locus_tag="ACHKNP_RS19055"
                     /old_locus_tag="pgaptmp_003811"
     CDS             complement(3353..4033)
                     /gene="rnc"
                     /locus_tag="ACHKNP_RS19055"
                     /old_locus_tag="pgaptmp_003811"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGU4705901.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4306..5280)
                     /gene="lepB"
                     /locus_tag="ACHKNP_RS19060"
                     /old_locus_tag="pgaptmp_003812"
     CDS             complement(4306..5280)
                     /gene="lepB"
                     /locus_tag="ACHKNP_RS19060"
                     /old_locus_tag="pgaptmp_003812"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGU4705902.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5296..7095)
                     /gene="lepA"
                     /locus_tag="ACHKNP_RS19065"
                     /old_locus_tag="pgaptmp_003813"
     CDS             complement(5296..7095)
                     /gene="lepA"
                     /locus_tag="ACHKNP_RS19065"
                     /old_locus_tag="pgaptmp_003813"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGU4705903.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7293..7772)
                     /gene="rseC"
                     /locus_tag="ACHKNP_RS19070"
                     /old_locus_tag="pgaptmp_003814"
     CDS             complement(7293..7772)
                     /gene="rseC"
                     /locus_tag="ACHKNP_RS19070"
                     /old_locus_tag="pgaptmp_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HGU4705904.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKVSCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7769..8725)
                     /gene="rseB"
                     /locus_tag="ACHKNP_RS19075"
                     /old_locus_tag="pgaptmp_003815"
     CDS             complement(7769..8725)
                     /gene="rseB"
                     /locus_tag="ACHKNP_RS19075"
                     /old_locus_tag="pgaptmp_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HGU4705905.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8725..9375)
                     /gene="rseA"
                     /locus_tag="ACHKNP_RS19080"
                     /old_locus_tag="pgaptmp_003816"
     CDS             complement(8725..9375)
                     /gene="rseA"
                     /locus_tag="ACHKNP_RS19080"
                     /old_locus_tag="pgaptmp_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HGU4705906.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9408..9983)
                     /gene="rpoE"
                     /locus_tag="ACHKNP_RS19085"
                     /old_locus_tag="pgaptmp_003817"
     CDS             complement(9408..9983)
                     /gene="rpoE"
                     /locus_tag="ACHKNP_RS19085"
                     /old_locus_tag="pgaptmp_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HGU4705907.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9980..10135)
                     /gene="rseD"
                     /locus_tag="ACHKNP_RS19090"
                     /old_locus_tag="pgaptmp_003818"
     CDS             complement(9980..10135)
                     /gene="rseD"
                     /locus_tag="ACHKNP_RS19090"
                     /old_locus_tag="pgaptmp_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HGU4705908.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10391..12013
                     /gene="nadB"
                     /locus_tag="ACHKNP_RS19095"
                     /old_locus_tag="pgaptmp_003819"
     CDS             10391..12013
                     /gene="nadB"
                     /locus_tag="ACHKNP_RS19095"
                     /old_locus_tag="pgaptmp_003819"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HGU4705909.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11998..12735)
                     /gene="trmN"
                     /locus_tag="ACHKNP_RS19100"
                     /old_locus_tag="pgaptmp_003820"
     CDS             complement(11998..12735)
                     /gene="trmN"
                     /locus_tag="ACHKNP_RS19100"
                     /old_locus_tag="pgaptmp_003820"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HGU4705910.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12867..14201
                     /gene="srmB"
                     /locus_tag="ACHKNP_RS19105"
                     /old_locus_tag="pgaptmp_003821"
     CDS             12867..14201
                     /gene="srmB"
                     /locus_tag="ACHKNP_RS19105"
                     /old_locus_tag="pgaptmp_003821"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HGU4705911.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT         3419 a         3813 c         3552 g         3453 t
ORIGIN      
        1 tatagtatct gccaagacac ttatttatag ttattaaagg cgcgtccgat tggttcaccg
       61 gacgcacctt aagtacgttt ccttgtgtta taagaacaga aggatcagct gtaaaacagc
      121 aatgatgatt ttgatgaccc gtttaatcag gtatcggcaa tcagtcattc gtttttcctt
      181 aaacaaggaa tgcagccgtt cagattagcc cttacatctc cccaaaactg aacgtgcgag
      241 ttattgaggg tgcatgctgc actccacacc agagctttga cgacaccact cgtttcaatg
      301 ggggaattct gtggcatggt gtaaagcaca gcaaaatctt caataacgaa gccaatttta
      361 atgtacttac gaattggcgg tcacgttaat ctttccatca atattgcttt cttcgtaaag
      421 gctcgagttt ttatgctaaa gattgcaagt tgcttgtaaa agataagtac actgatccat
      481 aatcgctgtt gttgagggtg catgctgcac aaaattaaag ttaaaaagta aaacccccgt
      541 tccttaccag ttcgggggtt ttacttttta aagagaacgg tattatttta actttcaata
      601 attaccgtgg cacaagcata gtggcgttca tctgccagcg ttacatgcat atttacaacg
      661 ccgagctttt ccgccagttt taatgcctca ccccatagcc gtagccgtgg tttgccgagc
      721 tcatcattga atacttcaaa ttgattaaac gccagaccat tgcggatccc ggtgccaaac
      781 gcttttgctg cggcttcttt cacagcaaaa cgcttcgcca gaaaacgcac cggctggtgg
      841 tgcgttttcc agattgccca ttcgttatcg cttaatacgc ggcgtgccag gcgctcaccg
      901 gatcgggcga tcactgcttc tatgcgagcg atctctacga tatccgtgcc taaacctaat
      961 attgccatta gccacgcgct tccagcatca gacgcttcat ttctgccacc gcatctttca
     1021 gtccggtcat cactgcacga ccaataatgg catgaccgat attcagttca tgcatctcag
     1081 ggatggcggc aatagctttc acgttgtgat aggtcagacc gtgaccagcg ttaactttca
     1141 gaccgagact agcggcaaag gtcgcggctt tggcgatacg cgccagctct tgcgcctgtt
     1201 cggcgtcagt tttggcatca gcatagcaac cggtgtggat ctcgataaac ggtgcgccaa
     1261 cctctgccgc agctttgatc tgctcttcat cggcgtcgat aaagagagaa acctggatcc
     1321 cggcatctgc cagacgtttg caggcatcgc gcattttgtc acgctgccct gcgacgtcca
     1381 ggccgccttc ggttgttact tcctgacgct tttccggtac cagacagcaa aaatgtggct
     1441 tcgtctcaac ggcgatcgcc agcatctctt cggtcaccgc catctccaga ttcatgcggg
     1501 tatccagcgt ctgacgcagg atgcgcacat cacggtcagt aatgtggcgg cgatcttcac
     1561 gtaaatgcac ggtaatgccg tccgctcccg cctgctcagc aataaacgcc gcctgcaccg
     1621 gatccgggta agcagtaccg cgtgcgttac gcagcgttgc aatatggtca atgttgacgc
     1681 ctaacagtaa ttcagccatg acaatcctca tcattcataa tgtgttttca ccgttcgctt
     1741 aggcataaac tgccggaaca gttccctgct ctttaatggt ttaccgccaa gatacggctt
     1801 aagcgccatg cgggtaaagc gtttcgcggc gcgtagtgtg tctgcgtcag gaaattcccg
     1861 cgcgtttaac gcttttaact gccttccggt gaacgtttta ttgtcgataa cgacgcttgc
     1921 gataaaccct ttttcttcgc gataacgata cgtcatggtg tcatctaccg gctcgccgct
     1981 acccgcacaa tgggtaaaat tgacgccata gcccagatgc ccgagcagtg ccagttcaaa
     2041 gcggcgcagc gcgggttctg gcgtaccagt ggcacctgca agagactgaa tgcagtgcaa
     2101 gtaatcgaaa aagagttcag agaagcgcgt ctcgtattcc agtacgcggg agagaagttc
     2161 gttgatgtac agaccgctgt aaagcgtgat accgcttaat ggcagcgcca gcgagacagc
     2221 ttcagcactg cgcagcgttt tgacttcgcc acgcccgccg aaacgtagca agagaggggt
     2281 gaaaggctgt aatgcacctt tcagagtaga gcgtttagag cgtgcgcctt tggcaaccag
     2341 acgcacgcga cccgattcct ccgtgaagac gtccagcatc aggctggttt cgctccacgg
     2401 gcgactatgc aggacaaatg cgcgctgcca gccttccatc ggagtgactc ttaaagatcg
     2461 tcaacgtaac cgagactgcg cagtgcgcgt tcgtcgtcgg cccaaccgga tttcactttt
     2521 acccacagtt caaggtggac aggcgcttcg aacatttcct gcatgtcttt acgcgcttca
     2581 atcccgatgg ttttgatctt ggcccctttg ttgccaataa ccatcttctt ctgcccttca
     2641 cgctcaacga gaatcaaacc attgatgtca taaccaccgc gttcgttaga gacgaaacgt
     2701 tcgatctcca cggtcacgga gtacggcagt tcagcgccga ggaaacgcat cagtttttcg
     2761 cggatgattt cagacgccat aaaacgctgt gagcgatcgg tgatgtaatc ttccgggaag
     2821 tgatgggtcg cttcagggag atgcttacgc acgattgctg cgatagtgtc gacattcagc
     2881 ccggtttcag cagagatcgg cacgatatcg aggaagttca tctgacttgc caggaactgt
     2941 aagtgcggca gcagatcggc tttttcctgc acgttatcca ctttgttcac cgcgaggatc
     3001 accggcgctt tgccatcgcg cagtttgttg agcaccattt cgtcgtccgg cgtccagcgg
     3061 gtgccttcaa cgacaaaaat caccagctca acgtcgccaa tagagctgct cgccgctttg
     3121 ttcatcaggc ggttaatagc gcgtttttct tccatatgca ggccaggggt atcaacgtag
     3181 atcgcctgat acgcgccttc agtatggatc cccacaatgc ggtgacgagt tgtttgcgct
     3241 ttgcgggagg tgatggagat cttctgcccc agcagtttgt tcaacaatgt ggatttgcca
     3301 acgttcggac gtccgacgat ggcaataaat ccgcagtaac ttttatcgat gctcattcca
     3361 gctccagttt tttcaacgcc tgttcggcgg cagcctgctc agccttacga cggcttgaac
     3421 ctgtgccaac caccggttca ctcaggccgc tgacctggca gtggatagta aattcctgat
     3481 cgtgcgcttc gccacgtacc tggactacca gataagtcgg cagcggcaga tggcgaccct
     3541 gcaaatattc ttgcaagcgc gttttcggat ctttttgttt atcgcctggg ctaatttcgt
     3601 ccaaacgagt ttgataccag ttgaggatta atttctcgac ggtttgaata tcactgtcga
     3661 ggaatacacc accaattaat gcttcgacgg tgtcggcgag aattgactca cgacgaaatc
     3721 caccgctttt aagttcacct ggccctaaac gtaagcactc gcctaactca aattcgcgcg
     3781 ccagttccgc cagcgtattg ccacggacca gcgtggcgcg catccggctc atatcgcctt
     3841 catccacacg agggaaacgg tgataaagcg cattggcgat aacgtagctc agaatagagt
     3901 cgcctaaaaa ttctaaacgc tcgttatgtt tgctgctggc actacgatga gttaatgcct
     3961 gctgcaacag ttcctgatga ttaaaagtgt agcccagctt ccgttgaagc cgattaatta
     4021 cgatggggtt catgcgatac caataaataa atgcgtcaac aattcagcac acgaaacaga
     4081 cctgatatac atggctctgc taactgcttc gctgcagttt cagtttaccg gtatatgggg
     4141 accaacgctg tttcgtgtgc cgtggcaacc tggaggtgcc aaccttaaac ttcgggggaa
     4201 tattctatac acaacgacgg gggatgtcgt tagccacggg agatttatct cataaataat
     4261 tcacgttgtc gccataacgg cgacaacgtg aacgaagatg ggctattaat ggatgccgcc
     4321 aatgcgactt aagcgcacac ctgtcggcca ttcgccttct tgcttatcga aactcatcca
     4381 gatagccgtt gcccgaccga ccagattcgc ttcaggcaca aaaccccagt aacggctgtc
     4441 cgcgctgttg tcgcggttgt cgcccatcat gaagtattgt cccggcggaa caatccaggt
     4501 tgccagttgt tgccctggct gctggtaata catccccacc tggtcctgcg caatcggcac
     4561 tgtcagaatt cggtgcgtca catcacccag tgtctcttta cgctcggaaa gacgaattcc
     4621 attttctttg gtttcgtttt tcggcacttc aaagaatccg ctggtcgctt ccccaccatt
     4681 acggcgtgag aaggtctgaa cgaaatcgct cggttccacg ttggagtagg tgaccggtag
     4741 cgcgttttca cacgcctggc cggaactgca tcccggttga atcgtcagct cttttgagac
     4801 cggatcgtaa gtgactttat cgcccggtaa acccaccgcg cgcttgatgt aatcaagctt
     4861 tggatcttcc ggatatttaa agaccacgat atcgccgcgt ttcggatgac cggtttcgat
     4921 cagcgttttc tggtagatag gatctttaat gccataagca aacttctcta ccagaataaa
     4981 atcaccgatt aacagagtcg gcatcatcga acctgacggg atctggaacg gttcataaat
     5041 aaacgaacgt acaatcaata cgatagccag caccggaaaa acagaagcac cggtttccag
     5101 ccagccaggc ttcggcgcaa cctttttcag ggttgcttta tccagtgagt caccggcagc
     5161 cgcctgcgcc gctgcctgac gttcccgccg tttaggtgcg aaaaagaatt tatccacgca
     5221 ccataaaatg cccgtcacca gtgtggcaat caccagaatc agggcaaaca tattcgccat
     5281 gccaactcct aagggttatt tgttgtcttt gccaacgtgc agaatggcga ggaacgcttc
     5341 ctgcggcagc tcgacgttac cgatctgctt catgcgtttc ttaccttctt tctgcttctg
     5401 cagcagcttt ttcttacggc tgatatcgcc gccataacat ttagccagta cgtttttacg
     5461 tagctgtttc acggtggatc gcgcaataat gtgcgtacca atcgctgcct gaatggcgat
     5521 atcaaactgc tggcgtggga tcagatcttt catcttctcc accaactcgc gaccgcggtt
     5581 ttgcgaatta tcacggtggg tgatcagcgc caacgcatca acacgttcac cgttgattaa
     5641 tacgtctaca cgtaccatgt cggacgcctg gaagcgtttg aagttgtaat ccagagacgc
     5701 ataaccacgc gaggtagatt tcaggcgatc gaagaagtcg agcaccactt ccgccatcgg
     5761 gatctcgtac gtcagcgcca cctgattacc gtggtaaacc atattggtct gcacgccgcg
     5821 tttttctacg cacagcgtaa taacgttgcc gagatatgcc tgcggcagca gcatgtgaca
     5881 ttcggcaatc ggctcgcgca gttcgtagat gttatttacc gctggcagct tggatggact
     5941 gtcgacgtaa ataacttctc ttgacgtggt ttcaacttca tacactacgg tcggcgcagt
     6001 ggtgatcaga tccagatcgt attcacgttc cagacgttcc tggatgatct ccatgtgcag
     6061 caggccgagg aagccgcagc ggaaaccaaa gcccagcgcg ctggagcttt ccggctcata
     6121 gaacagtgaa gcatcgttca ggctgagttt acccagcgcg tcacggaagg cttcatagtc
     6181 gtcggaactt accgggaaca gaccggcgta tacctgcggt ttgactttct taaagccagg
     6241 cagcgccttt tctgccggat tacgcgccag cgttaaggta tcgccgactg gagcgccgtg
     6301 gatatcttta atcgcacata ccagccagcc tacttcgcca catttcagtt cagtgcggtc
     6361 aacctgtttc ggcgtgaaga tgcccagacg gtcggcgtta taggtctgcc cggtactcat
     6421 gactttcact ttgtcgccct tacgcagggt gccgttttta atacggataa gtgaaacaac
     6481 gcccaggtag ttgtcgaacc atgagtcgat aattagtgcc tgcaacgggc cttccggatc
     6541 gccttccggc ggcggaatgt cgcgcaccag acgttcgaga acgtcctgca cgccaacgcc
     6601 ggttttcgct gaacagcgca ccgcgtcggt ggcgtcgatg ccgacgatat cttcaatttc
     6661 ttccgccacg cgttcaggat cggctgccgg caggtcaatc ttgttcagta ccggcaccac
     6721 ttcgagatcc atttccatgg cggtgtagca gtttgccagg gtttgcgctt ctacgccctg
     6781 cccggcgtcg accaccagca atgcaccttc acaggcagcc agcgaacggg aaacttcata
     6841 ggagaagtca acgtggcccg gggtgtcgat aaagttcagc tgataggttt cgccgtcaga
     6901 cgctttgtag tccagcgtca cgctttgcgc tttgatggtt atgccacgct cacgctcaag
     6961 atccatggaa tcgagaacct gcgcctccat ttcacggtca gacaggccac cgcagatctg
     7021 gataatacgg tcagacagcg tcgatttacc gtggtcaatg tgagctatga tcgaaaagtt
     7081 acgtatattc ttcataaagt atgattattg tgccttaatg cccggttaac caggctttta
     7141 gaagtcgctg ttctgagctt aacgtctgta ttaatagaaa cgccgcattc tacactacaa
     7201 cattgaggcg aggaaatgtt cataccgtat ggattgtggt atctggaaac gtcctcgcat
     7261 ttgttatgca aaatgcaaca aagccagtga aatcactggc tcgcgtcttc cgaagatgtt
     7321 tcaaatcgca caaggccagg cggcaatgcc acgcttaaga tgatcggttg ccattctgcc
     7381 cgggcagcaa acttacgcga gtagccgcgc gcaatcagga acccgccaat accgccgagg
     7441 accgcaccac ataatgctgc gacgtcagaa gcaaagagta gctgaaatag cgaagcgatg
     7501 aggaataatc ccaccagcgg cgacatataa accagtaatg cggagctaag caggctgcct
     7561 tcggcgatcc ccaattccac tttttgcccc ggcactaacg gctcatcaca gggtacgaca
     7621 atggtatgcg tggtttgcgg gccaagttta tttaacacgc ggctaccgca accggcgcgt
     7681 gaagcgcagc tgctgcatga aactttaaca tcacaactga ccagcgcctg cccgttttgc
     7741 caggagacga cggtagccca ctctttgatc attgcgctgc cccgaactta atattctcgg
     7801 caatgcgttt cgccgtttgc ggcggcagtt caccgacaat ggtgatttcg gcgttatcac
     7861 gtacgcttgt actgacggtt ctgcgtccgg tgcgcaacat ctgatcggtg ctcgatggcg
     7921 tagcgcggtt aacgtttacc gagaagctga ataatccgtc ggaatagaga cgtgattcga
     7981 taggcatgtt gtccatcgtc ggtagcggac gtcgactact ggaaacttcg ctaaaaccct
     8041 gtggcaacca ggttggcgtc cagctgaatt tagctttttc acctacagga acagaaagca
     8101 acggcggcaa atttgccttc gccagcgtct gcatactgct actgatatcc tgattgacgt
     8161 taaaagcaat cacgcgaaat tgttccagcg tttcaccatc gcgatcaagg agatcaaccc
     8221 tcatcggtaa tttcgattcg atgtccatcc acacgatgta gctgtagcgt gtaccatctc
     8281 gggcaaccac gcgaatgact tcgcaaagac gatcagcaat acgcgtgcgc ccgacggaga
     8341 taaagtcgta gtaaggagaa aggcgtttga aatcggtata gatgagcgat ggcagagaat
     8401 caacaatgta atcgccatta agcgtgaacg gttcaagtcc cggttcaaaa tagctgattt
     8461 cattgccacg ctgtaccact tcccggcgcg ggccatccat ttgcaacaat tgtgcaagag
     8521 gacggttatc gaggcgtgca tgtcgataac gcagagactc aacaccctgt ttattgatgc
     8581 tgatgaatga cagctcgtaa ttcagtgact gactggccag gttcatctgc tgtaataacg
     8641 ccccggacgc gggagtggcc gaggcgttag cagagaataa caggctacct gtcactaatg
     8701 acatggcaaa ccaaagttgc ttcattactg cgattgcgtt cctaaagttt gaattcctgg
     8761 cacctgtaca gcggcttgct gggtttgcgc ctgctcaaac tgaagctgtt cagagtggag
     8821 tcggcgttgc agttcgtaat cctgcaacat tgcattaatg cgacgacgct gctcctgtac
     8881 ctgctgctgt tgaccattgt ttgcggtcgc ttcagaaggt actcccaggc ttaccgggct
     8941 ggctttaccc atcatcggca gtgtattaaa taccggcgtt tcgggctgct gggacgtttc
     9001 agattgtcca ttatagtgct ggacgccaac gataactgca agcgatacgc atgctgctac
     9061 gcccatttgg gtaagctgtg ccgcccacgg acgtactttc tgccagaatg gcattttctg
     9121 ccattgatgc ggcgcaggct gggcttccgg gatcaatgtc gccggttgac gtactggctc
     9181 ttcttcaatg gcggccatca cgcgtgaaga gatatcgaaa tggagcacct cgggagtatc
     9241 accccgcatt gagtcacgga ttaagtgata gctttcccag gttttctgca tttctgggtt
     9301 atgagccagt tcgttaagca gctcactatc cagcgtttcg ccatccatta aagcggaaag
     9361 ttgttctttc tgcatgccta atacccttat ccagtatccc gctatcgtca acgcctgata
     9421 agcggttgaa ctttgttatc aatagcttcc ctcgctcgga agatacgtga acgcaccgta
     9481 cctaccggac aatccatgat agcggctatc tcttcatagc tcaggccatc cagctcccgc
     9541 aaggttattg ccatgcgcaa atcttccggg agggactcaa tagttcggaa aactatctgt
     9601 ctcagttctt ctgacaacat taagttctca gggttcgaaa tttctttcaa cgcgccgcca
     9661 ctttcgaagt tttcagcttc aatggcatcc acatcactgg aaggtggacg acgcccctga
     9721 gcaaccaggt aatttttcgc tgtatttaca gcaatccgat acagccatgt ataaaaagcg
     9781 ctatctcccc ggaacgaatc cagcgcacga taggctttaa taaaagcttc ttgtaccaca
     9841 tcgggaacat cacccgacgg cacatagcgg gaaaccagac tcgccacttt atgctgatag
     9901 cgcactacca gtaggttaaa ggctttctga tctcccttct ggacccgttc aaccaggacc
     9961 tggtccgtta actgctcgct catccgaggt aaagtctccc caaaccaaat ttccacgcgc
    10021 tatcgaaacg ccactccatt agctgcaatt tgagcaagca aagggttaga gtgtctcgtt
    10081 tttgtaaagt tccgtaacgc atctgttttt gtttgtcatg ctgtagacgg atcattatct
    10141 atcattataa gtctacagaa tctgaacatc gcattatctg tgtagaaatg cccatttaac
    10201 tgcctgaaga gtaacccaac ggccttttta tttcaccacc taatcctcca ccagccagta
    10261 acttctcttt ttctcgccgc cctgcgtcag cgtgtttagc cactgtaaca aatattaaaa
    10321 tagcaggtgt ttattcgcac aacatgatgc tatgctgacc aaacggtgtt tagtaaatta
    10381 aacaaagaaa atgaatactc tcccagaaca ttcatgtgac gtgttgatta tcggtagcgg
    10441 cgcagccgga ctttcactgg cgctacgcct ggctgaccag catcaggtca tcgttctaag
    10501 taaaggcccg gtaacggaag gttcaacatt ttatgcccag ggcggtattg ccgccgtgtt
    10561 tgatgaaact gacagcattg actcgcatgt ggaagacaca ttgattgccg gggctggtat
    10621 ttgcgatcgc catgcagttg aatttgtcgc cagcaatgca cgatcctgtg tgcaatggtt
    10681 aattgaccaa ggggttttgt ttgataccca cgttcaaccg aatggcgaag aaagctacca
    10741 tctgacccgt gaaggtggac atagtcaccg tcgtattctt catgccgccg acgccaccgg
    10801 tagagaagta caatccacgc tggtgagcaa ggcgcagaac catccgaata ttcgtgtgct
    10861 ggagcgcagc aacgcagttg atctgattgt ttctgacaaa attggcctcc cgggcacgcg
    10921 acgggttgtt ggcgcgtggg tatggaaccg aaataaagaa acggtggaaa cctgccacgc
    10981 aaaagcagtg gtactggcaa ccggcggtgc gtcgaaggtt tatcagtaca ccaccaatcc
    11041 ggatatttct tctggcgatg gcattgctat ggcgtggcgc gcaggctgtc gggttgccaa
    11101 tctcgaattt aatcagttcc accctaccgc actgtatcac ccacaggcac gcaatttcct
    11161 gttaacggaa gcactgcgcg gcgaaggcgc ttatctcaag cgcccggatg gcacgcgttt
    11221 tatgcccgat tttgatgagc gcggcgaact ggccccgcgc gatattgtcg cccgcgccat
    11281 tgaccatgaa atgaaacgcc tcggcgcaga ttgtatgttc ctcgacatca gccataagcc
    11341 cgccgatttt attcgccagc atttcccgat gatttatgaa aaattgctcg ggctggggat
    11401 tgatctcaca caagaaccgg taccgattgt gcctgctgca cattatacct gcggtggtgt
    11461 aatggttgat gatcatgggc gtacggacgt cgagggcttg tatgccattg gcgaggtgag
    11521 ttataccggc ttacacggcg ctaaccgcat ggcctcgaat tcattgctgg agtgtctggt
    11581 ctatggctgg tcggcggcag aagatatcac cagacgtatg cctgatgccc acggcgtcag
    11641 tacgttaccg ccgtgggatg aaagccgcgt tgagaaccct gacgaacggg tagtaattca
    11701 gcataactgg cacgagctac ggctgtttat gtgggattac gttggcattg tgcgcacaac
    11761 gaagcgcctg gaacgcgccc tgcggcggat aaccatgctc caacaagaaa tagacgaata
    11821 ttacgcccat ttccgcgtct caaataattt gctggagctg cgtaatctgg tacaggttgc
    11881 cgagttgatt gttcgctgcg cgatgatgcg caaagaaagc cgggggctgc attttacgct
    11941 ggattacccg gaactgctca cgcattccgg tccatcgatc ctttcccccg gcaatcatta
    12001 cataaacaga taaaaagcct gggtcagcgc cgtatacgct tcggaatagt tctggtctgg
    12061 cccacgaatg actaagcgat cgctaaagca ttctcccgcc tgcggggaga atgccagcag
    12121 cacccgatgc ggcagtcgcg cttcgttttc cgccacatcc gtccgcaaac gtaaatgcca
    12181 gcccatgctt aatgccagtt cagtaaaacc attaccaatc tgctctggca gcaccacgca
    12241 gaaaaatccc tcttcggtaa tgcactccgc cgcacaggtc agcagcgatg ggtgatcaag
    12301 cgtagtggta tagcgagcct gttcccgttg aggtgtcgcg cactctactc cctgctgata
    12361 gtaaggtggg ttactgatga ttaaatcgaa gcgtactgtc tgctgtgtga tccactgctg
    12421 aatatccgcc gtatggacgt taatccgctc tgcccacggg gactggttga tattttcctg
    12481 cgcctgcgcg gcagcttcac tttccagctc aacggcatca attattacgc tgtcactggt
    12541 tcgctgcgcc agcatcagtg ccagcaaccc gctacccgcg ccgatatcaa ggcaacgttt
    12601 caccccagcc accggtgccc atgcgcccag caaaatgcca tccgttccca ctttcatcgc
    12661 acagcgatcg tgagcaacaa aaaactgttt aaaagtaaat ccattacgac gaagcacgga
    12721 tgtagactgt gacatgaaaa taaaaccttg caggaaaaac ggcgatagca ccgggtgaga
    12781 acaatacccg agaagcgata tccatacaaa cagatgaaga ttgcagccgt aacgtctata
    12841 atcagcgccc cacacagagg tagaacatga ctgtaacgac tttttccgaa cttgaactcg
    12901 acgaaagcct gctggaagcc ctccaggata aaggtttcac tcgcccgacc gccattcagg
    12961 ctgccgccat tccgcctgcg ctcgatggcc gtgatgtact cggttctgcg ccgacaggca
    13021 ccggtaaaac ggcggcgtat ctgctgccag cgttgcagca cctgctcgat ttcccgcgta
    13081 agaaatccgg tccgccgcgt attttgatcc tcaccccaac tcgcgagctg gcgatgcagg
    13141 tggccgatca tgcccgcgaa ctggcgaaac atacgcatct ggatatcgcc accatcaccg
    13201 gcggcgtagc ctatatgaac cacgcggaag tgttcagcga aaatcaggac atcgtggtcg
    13261 ccacgaccgg acgtctgctg caatacataa aagaagagaa cttcgattgc cgcgcggttg
    13321 aaacgctgat cctcgacgaa gcagaccgta tgctggatat gggcttcgct caggatatcg
    13381 aacatattgc tggcgaaacg cgctggcgta aacagaccct gctcttttcg gcaacgctgg
    13441 aaggcgatgc gattcaggac tttgccgagc gtctgctgga agatccggtg gaggtttctg
    13501 ccaatccgtc cacccgtgag cgcaaaaaaa ttcatcagtg gtattaccgc gccgatgacc
    13561 tggaacataa aaccgcattg ctggtgcatc tgttaaaaca gccggaagcg acccgctcaa
    13621 ttgtgtttgt gcgtaagcgt gaacgtgtgc atgagctggc aaactggctg cgcgaagcgg
    13681 gcatcaacaa ctgctatctc gaaggtgaga tggtacaagg caagcgtaac gaagcgatca
    13741 agcgtttgac cgaaggtcgc gtaaacgtac tggttgcaac cgatgttgcc gcgcgcggta
    13801 tcgacattcc tgacgtcagc cacgtcttta acttcgatat gccgcgcagt ggcgatactt
    13861 atttgcaccg tatcggacgt accgcgcgcg ccggtcgtaa aggcaccgca atttcgctgg
    13921 tggaagccca tgaccatttg ctgctgggca aggttggccg ttacattgaa gagccgatta
    13981 aagctcgcgt tattgatgag ttacgcccga aaacgcgtgc gccaagcgag aagcagaccg
    14041 gcaagccatc gaagaaagta ctggcaaaac gcgccgagaa gaaaaaagct aaagagaaag
    14101 agaaaccgcg tgtgaaaaaa cgccatcgcg acaccaaaaa tattggtaag cgccgtaaac
    14161 caagcggaac gggcgtgcca ccgcaaacga cagaagagta atctcaatgc cgggtttaag
    14221 cctggtatta aaagtgc
//
LOCUS       DAZFWL020000122        14233 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFWL020000122 DAZFWL020000000
VERSION     DAZFWL020000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.122"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            257..1399
                     /gene="yghO"
                     /locus_tag="ACHKNP_RS19110"
                     /old_locus_tag="pgaptmp_003822"
     CDS             257..1399
                     /gene="yghO"
                     /locus_tag="ACHKNP_RS19110"
                     /old_locus_tag="pgaptmp_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HGU4705912.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGALFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1406..2170)
                     /gene="glcC"
                     /locus_tag="ACHKNP_RS19115"
                     /old_locus_tag="pgaptmp_003823"
     CDS             complement(1406..2170)
                     /gene="glcC"
                     /locus_tag="ACHKNP_RS19115"
                     /old_locus_tag="pgaptmp_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HGU4705913.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2421..3920
                     /gene="glcD"
                     /locus_tag="ACHKNP_RS19120"
                     /old_locus_tag="pgaptmp_003824"
     CDS             2421..3920
                     /gene="glcD"
                     /locus_tag="ACHKNP_RS19120"
                     /old_locus_tag="pgaptmp_003824"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HGU4705914.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3920..4972
                     /gene="glcE"
                     /locus_tag="ACHKNP_RS19125"
                     /old_locus_tag="pgaptmp_003825"
     CDS             3920..4972
                     /gene="glcE"
                     /locus_tag="ACHKNP_RS19125"
                     /old_locus_tag="pgaptmp_003825"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HGU4705915.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4983..6206
                     /gene="glcF"
                     /locus_tag="ACHKNP_RS19130"
                     /old_locus_tag="pgaptmp_003826"
     CDS             4983..6206
                     /gene="glcF"
                     /locus_tag="ACHKNP_RS19130"
                     /old_locus_tag="pgaptmp_003826"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HGU4705916.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6211..6615
                     /gene="glcG"
                     /locus_tag="ACHKNP_RS19135"
                     /old_locus_tag="pgaptmp_003827"
     CDS             6211..6615
                     /gene="glcG"
                     /locus_tag="ACHKNP_RS19135"
                     /old_locus_tag="pgaptmp_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HGU4705917.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6637..8808
                     /gene="glcB"
                     /locus_tag="ACHKNP_RS19140"
                     /old_locus_tag="pgaptmp_003828"
     CDS             6637..8808
                     /gene="glcB"
                     /locus_tag="ACHKNP_RS19140"
                     /old_locus_tag="pgaptmp_003828"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HGU4705918.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPECVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFQVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDAAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9036..9281
                     /gene="traT"
                     /locus_tag="ACHKNP_RS19145"
                     /old_locus_tag="pgaptmp_003829"
                     /pseudo
     CDS             9036..9281
                     /gene="traT"
                     /locus_tag="ACHKNP_RS19145"
                     /old_locus_tag="pgaptmp_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            complement(9314..11056)
                     /gene="cptA"
                     /locus_tag="ACHKNP_RS19150"
                     /old_locus_tag="pgaptmp_003830"
     CDS             complement(9314..11056)
                     /gene="cptA"
                     /locus_tag="ACHKNP_RS19150"
                     /old_locus_tag="pgaptmp_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU4705919.1"
                     /translation="MQKTLTKHEHVFSWKALGWALIYFWYFSTLLQIIICLTGYSGTN
                     GLRDSLLFSSLWLIPVFLFPERIKVTAALIGVVLWGTSLAALCYYLIYGQEFSQSVLF
                     VMFESNTSEASEYFSQYFSLKILFISTIYTAIAILLWTRLSPIYIPSYWRYIIPLFII
                     YGLILHPVLINTVVKNRTIDNTLGRLASRMEPAAPWQFITGYYQYRLQLNSLNRLLEE
                     NNALPALKNFNDISGDTPRTLVLVIGESTQRGRMSLYGYPRKTTPGLDELYKTEQNMF
                     VFNNVVSSRPYTIEVLQQVLTFADEQQPELYLTQPSLMNIMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLAPFSEVLADPAPKKFVIIHLLGT
                     HIKYKYRYPEGQGVFDGDREHAPEGLTDNELELYNDYDNANIFNDHVVATLIKTFKAT
                     APDGFLFYFSDHGEEVFDTPPHKMLGRNEDTPTRPMYNIPFILWASEKWLLNRHHTFS
                     EYINRKYSLADFIHTWSDLAGLRYDNYEPERSILNQHFKEITRWIGNPYKKNALTDYD
                     TLPYAELRVNQASN"
     gene            complement(11475..12239)
                     /locus_tag="ACHKNP_RS19155"
                     /old_locus_tag="pgaptmp_003831"
                     /pseudo
     CDS             complement(11475..12239)
                     /locus_tag="ACHKNP_RS19155"
                     /old_locus_tag="pgaptmp_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            12459..12816
                     /locus_tag="ACHKNP_RS19160"
                     /old_locus_tag="pgaptmp_003832"
                     /pseudo
     CDS             12459..12816
                     /locus_tag="ACHKNP_RS19160"
                     /old_locus_tag="pgaptmp_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(13070..>14233)
                     /gene="iha"
                     /locus_tag="ACHKNP_RS19165"
                     /old_locus_tag="pgaptmp_003833"
     CDS             complement(13070..>14233)
                     /gene="iha"
                     /locus_tag="ACHKNP_RS19165"
                     /old_locus_tag="pgaptmp_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HGU4705920.1"
                     /translation="ENKGRELVRNVLKRDKWGLAGQLRELKESNLILNSLLLTPLGES
                     HLVTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHLTDALALTAGSRYEHHE
                     QFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLHKGISGVSGQGKTNLLGNPD
                     LKPEESVSYEAGVYYDNPAGLNANITGFMTDFSNKIVSYSINDNTNSYINSGKARLHG
                     VEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVA
                     SWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKVTDALTLNA
                     AVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
BASE COUNT         3537 a         3466 c         3790 g         3440 t
ORIGIN      
        1 ttaaataatg agtttaagtt ttttgttttt attgtgaatg atatttaatt tatgaatata
       61 ttaagaagaa gccttgtccg gtcgccttaa atcgacaaaa ggcattgagg cgcttctgtg
      121 tagtctctaa ccttatcata atcattaata aatactattt attctttgtt tgtattcatt
      181 gggttgatta ttaaggtgtc gttcgttgct gttgcaacct gtttttcacc gttaattatt
      241 tgttatccac agagatatgg aatgcgatct gctgatgatt aaaattgaaa aagtaattaa
      301 taaaaatgac cttaaggcat ttatcgcttt tccgtcatca ctttatcccg acgatccaaa
      361 ctggataccc cctttattca tagaacgcaa cgagcatttg tctgcgaaaa atccggggac
      421 ggaccatatc atctggcagg cgtgggtggc aaaaaaagcg gggcagatag ttgggcgtat
      481 taccgcgcaa atagatacct tgcaccgtga gcgttacggt gaagataccg gtcatttcgg
      541 catgattgac gccattgatg atccgcaggt ttttgccgcg ttgtttggcg cagcggaagc
      601 gtggttgaag tcacaaggtg caagtaagat cagcggtcct ttcagcctga atatcaatca
      661 ggaaagcgga ttactgattg aaggttttga cacaccaccc tgtgcgatga tgccacacgg
      721 caaaccgtgg tatgccgcgc atattgaaca actgggttat cacaaaggca ttgatttact
      781 ggcgtggtgg atgcagcgaa ccgatctcac tttctctccg gcgctaaaaa aactgatgga
      841 tcaggtgcgc aaaaaggtga ccattcgctg catcaatcgt cagcggtttg ccgaagaaat
      901 gcagatcctg cgtgagattt tcaactctgg ctggcagcac aactggggat ttgtgccgtt
      961 taccgaacat gaattcgcga ccatgggcga tcaacttaaa tatctggtgc cggacgatat
     1021 gatctatatc gctgagattg attctgcacc ctgcgcgttt attgtcggct tgccgaacat
     1081 caacgaggcg attgccgatc tgaacggagc gctctttccc ttcggctggg caaaattgct
     1141 gtggcgtttg aaagtcagcg gtgtgcgaac tgcgcgagta ccgctgatgg gcgtgcgcga
     1201 cgagtatcag ttcagccgca tcggcccggt gattgcgctg ttattgattg aagccttacg
     1261 cgatccgttt gcccgccgga agatcgatgc gctggagatg tcatggatcc tcgaaaccaa
     1321 tacaggcatg aataacatgc tggaacgcat tggggcggag ccgtacaagc gttaccgatt
     1381 gtatgaaaag cagatttaat accagctaac tcaggttcat ctccagcggc accgccgagc
     1441 gaatcaaatg gtggccttcc agctcgatat cgtggagatt ctttttcacg gtccgcacat
     1501 gatcgcgtgc tgcgcgctgg gcgacgtgcg gcagccgctg caacaccgcg ttgtagatcc
     1561 tcgcatgctg gcgatcgatc tgcttttttt gctgtggtcg atggtagaga ttatttacgc
     1621 tggcaaacac tgaattaaac atcagatcgg tcaatgattg cagcgtaaac accagcacct
     1681 gattgtgaga agcctgacaa atggcgagat ggaaagcgtg atccaactgc gcatgttcga
     1741 tcagcgaaat ctctttgttg ttctcactgg cggcgagcat tttttcataa cagcgggtta
     1801 tcacaacaaa atcagcctgc gttcccagcg ttgccgccag ccttgccgat tcgccctcca
     1861 gtaatgcgcg aacgtcgagc agatcgtaca gcgttcgcgg ctgcgtactg aacagatgga
     1921 tcagcggact ggtgtcctgc acccgattaa gccgtgcgac acgagaatcg cgaccctgcg
     1981 ccgtttcaat aatcccgcgc ccgcgcagca cggtcagccc ttcacgcagt gcggagcgtg
     2041 agaagccgag cttttcacac agtcgacgtt ccgagggaag tggctgaccg accttcagta
     2101 cgccgtcgat aattaaccgt tcgatactct ctgcaaccac ttcgcaaata gggcgacgtt
     2161 catctttcat tcccggacct cgtgcacagg taggaccaat ttttctgttc ttaactcgca
     2221 aaacacgcac atcacgtaag tgattatgtt acatcaattt aacattgagt taaccaaggc
     2281 aaggtcacag agctggaaaa aaaatggtct gaccggtagc taaagagata gacgaaaacg
     2341 aaaagcccgc ttaataactg ttcacagaag cagcgcgcaa aaatcagctg ccacacaaca
     2401 caacaaagcg aagcctactc atgagcatct tgtacgaaga gcgtcttgat ggcgctttac
     2461 ccgatgtcga ccgcacatcg gtactgatgg cactgcgtga gcatgtccct ggacttgaga
     2521 tcctgcatac cgatgaggag atcattcctt acgagtgtga cgggttgagc gcgtatcgca
     2581 cgcgtccatt actggttgtt ctgcctaagc aaatggaaca ggtgacagcg attcttgctg
     2641 tctgccatcg cctgcgtgta ccggtggtga cccgtggtgc aggcaccggg ctttctggtg
     2701 gcgcgctgcc gctggaaaaa ggtgtgttgt tggtgatggc gcgctttaaa gagatcctcg
     2761 acattaaccc cgttggtcgc cgcgcgcgcg tgcagccagg cgtgcgtaac ctggcgatct
     2821 cccaggccgt tgcaccgcat aatctctact acgcaccgga cccttcctca caaatcgcct
     2881 gttccattgg cggcaacgtg gcagaaaatg ccggcggcgt ccactgcctg aaatatggtc
     2941 tgaccgtaca taacctgctg aaaattgaag tgcaaacgct ggacggcgag gcgctgacgc
     3001 tgggatcgga cgcgctggat tcacctggtt ttgacctgct ggcgctgttc accggatcgg
     3061 aaggtatgct cggcgtgacc accgaagtga cggtaaaact gctgccgaag ccgcccgtgg
     3121 cgcgggttct gttagccagc tttgactcgg tagaaaaagc cggacttgca gttggtgaca
     3181 tcatcgccaa tggcattatc cccggcgggc tggagatgat ggataacctg tcgatccgcg
     3241 cggcggaaga ttttattcat gccggttatc ccgtcgacgc cgaagcgatt ttgttatgcg
     3301 agctggacgg cgtggagtct gacgtacagg aagactgcga gcgggttaac gacatcttgt
     3361 tgaaagcggg cgcgactgac gtccgtctgg cacaggacga agcagagcgc gtacgtttct
     3421 gggccggtcg caaaaatgcg ttcccggcgg taggacgtat ctccccggat tactactgca
     3481 tggatggcac catcccgcgt cgcgccctgc ctggcgtact ggaaggcatt gcccgtttat
     3541 cgcagcaata tgatttacgt gttgccaacg tctttcatgc cggagatggc aacatgcacc
     3601 cgttaatcct tttcgatgcc aacgaacccg gtgaatttgc ccgcgcggaa gagctgggtg
     3661 ggaagatcct cgaactctgc gttgaagttg gcggcagcat cagtggggaa catggcatcg
     3721 ggcgagaaaa aatcaatcaa atgtgcgccc agttcaacag cgatgaaatc acgaccttcc
     3781 atgcggtcaa ggcggcgttt gaccccgatg gtttgctgaa ccctgggaaa aacattccca
     3841 cgctacaccg ctgtgctgaa tttggtgcca tgcatgtgca tcacggtcat ttacctttcc
     3901 ctgaactgga gcgtttctga tgctacgcga gtgtgattac agccaggcgc tgctggagca
     3961 ggtgaatcag gcaattagcg ataaaacgcc gctggtgatt cagggcagca atagcaaagc
     4021 ctttttaggt cgccctgtca ccgggcaaac gctggatgtg cgttgtcatc gcggcattgt
     4081 taattacgac ccgaccgagc tggtgataac cgcgcgtgcc ggaacgccgc tggtggcgat
     4141 tgaagcggcg ctggagagcg ccgggcaaat gctcccctgc gagccgccgc attatggtga
     4201 agaagccacc tggggcggga tggtcgcctg cgggctggcg gggccgcgtc gcccgtggag
     4261 cggttcggtc cgcgattttg tcctcggcac gcgcatcatt accggcgctg gaaaacatct
     4321 gcgttttggt ggcgaagtga tgaaaaacgt tgccggatac gatctctcac ggttaatggc
     4381 cggaagctac ggttgtcttg gcgtgctcac tgaaatctcg atgaaagtgt taccgcgacc
     4441 gcgcgcctcc ctgagcctgc gtcgggaaat cagcctgcaa gaagccatta acgaaatcgc
     4501 ccagtggcaa ctccagccgc tacccattag tggcttatgt tacttcgaca atgcgttgtg
     4561 gatccgcctt gagggcggcg aaggatcggt aaaagcggcg cgtgaactgc tggggggcga
     4621 agaggttgcg ggtcagttct ggcagcaatt gcgtgaacaa caactgccgt tcttctcgtt
     4681 accaggtacc ttatggcgca tttcattacc cagcgatgcg ccgatgatgg atttacccgg
     4741 cgagcaactg atcgactggg gcggagcgtt acgctggctg aaatcgacag ccgaggacaa
     4801 tcaaatccat cgcatcgccc gcaacgctgg cggtcatgcg acccgcttta gtgccggaga
     4861 tggtggcttt gccccgctat cggctccttt attccgctat caccagcagc ttaaacagca
     4921 gctcgaccct tgcggcgtgt ttaaccccgg tcgcatgtac gcggaacttt gaggagcagg
     4981 ctatgcaaac ccaattaact gaagagatgc ggcagaacgc gcgcgcgctg gaagccgaca
     5041 gcatcctgcg cgcctgtgtt cactgcggat tttgtaccgc aacctgccca acctatcagc
     5101 ttctgggcga tgaactggac gggccgcgcg ggcgcatcta tctgattaaa caggtgctgg
     5161 aaggcaacga agtcacgctt aaaacacagg agcatctcga tcgctgcctc acttgccgca
     5221 attgtgaaac cacctgtcct tctggtgtgc gctatcacaa tttgctggat atcgggcgtg
     5281 atattgtcga gcagaaagtg aaacgcccac tgccggagcg aatgctgcgc gaaggattgc
     5341 gccaggtagt gccgcgtccg gcggtcttcc gtgcgctgac gcaggtaggg ctggtgctgc
     5401 gaccgttttt accggaacag gtcagagcaa aactgcctgc tgaaacggtg aaagctaaac
     5461 cgcgtccgcc gctgcgccat aagcgtcggg ttttaatgct ggaaggctgc gcccagccta
     5521 cgctttcgcc caacaccaac gcggcaactg cgcgagtgct ggatcgtctg gggatcagcg
     5581 tcatgtcagc taacgaagca ggctgttgtg gcgcggtgga ctatcatctt aatgcgcagg
     5641 agaaagggct ggcacgggcg cgcaataata ttgatgcctg gtggcccgcg attgaagcag
     5701 gtgccgaggc aattttgcaa accgccagcg gttgcggcgc gtttgtcaaa gagtacgggc
     5761 agatgctgaa aaacgatgcg ttatatgccg ataaagcgcg tcaagtcagt gaactggcgg
     5821 tcgatttagt cgagcttctg cgcgaggaac cgctggaaaa actggcagtt cgcggcgata
     5881 aaaaactggc cttccactgc ccgtgtaccc tacaacatgc gcaaaagctg aacggcgaag
     5941 tggaaaaagt gttgcttcgt cttggattta ccttaacgga cgttcccgac agccatctgt
     6001 gctgcggttc agcgggaaca tatgcgttaa cgcatcccga tctggcacgc cagctgcggg
     6061 ataacaaaat gaatgcgctg gaaagcggca aaccggaaat gatcgtcacc gccaacattg
     6121 gttgccagac gcatctggcg agcgccggtc gtacctcagt gcgtcactgg attgaaattg
     6181 tagaacaagc ccttgaaaag gaataaaaaa atgaaaacta aagtcattct tagccagcaa
     6241 atggcgagtg caattattgc cgcaggtcag gaagaggcgc agaaaaataa ctggtcggtt
     6301 tccattgctg ttgccgatga cggcggtcat ctgctggcgt taagtcgcat ggacgattgc
     6361 gcgccgattg cggcttacat ctcccaggag aaagcgcgta ccgccgcgct ggggcgtcgt
     6421 gaaactaagg gctatgaaga gatggtgaac aacggacgta ccgcgttcgt gactgcgccg
     6481 ttactgacgt cgctggaagg cggcgtaccg gttgttgtgg atgggcaaat tattggtgcc
     6541 gtgggcgttt ctggtttaac cggagcacag gatgcgcagg tcgcgaaagc ggcagcagcg
     6601 gtgttggcga aataagcgaa aacgaggaga gaaacaatga gtcaaaccat aacccagggc
     6661 cgtttacgca ttgacgccaa ttttaaacgt tttgtggatg aagaagtttt accgggaaca
     6721 gggctggacg ctgcggcgtt ctggcgcaat tttgatgaga tcgttcacga tctggcacca
     6781 gaaaatcgtc agttgctggc agaacgcgat cgcattcagg cagcgcttga tgagtggcat
     6841 cgcagcaatc cggggccggt aaaagataaa gcggcctata aatctttcct gcgtgaactg
     6901 ggctacctgg tgccgcaacc ggagtgcgtg acggtggaaa ccacgggcat tgacagcgaa
     6961 atcaccagtc aggcagggcc acagctggtg gttccggcaa tgaacgcccg ctacgcgctg
     7021 aacgcggcga acgctcgctg gggctcactg tacgatgcgt tatacggcag cgacatcatc
     7081 ccgcaggaag gggcgatggt cagcggctac gatccgcagc gcggtgagca ggttatcgcc
     7141 tgggttcggc gtttcctcga tgaatctcta ccgctggaaa acggcagcta tcaggatgtg
     7201 gtggcgtttc aggtggtcga taaacaatta cgcatccagt tgaaaaatgg taaagaaacc
     7261 acgttacgta ccccggcgca gtttgtcggt taccgtggcg atgccgctgc gccgacctgc
     7321 attttgctga aaaataacgg cctgcatatt gaactgcaaa ttgatgccaa cggacggatt
     7381 gggaaagacg atgcggcgca catcaacgat gttatcgtcg aagctgcaat cagtaccatt
     7441 ctcgactgcg aagattcggt cgcggcggtt gatgcggaag ataaaatcct gctgtaccgc
     7501 aatctgctgg gcctgatgca ggggactctc caagagaaaa tggagaaaaa cggtcgacaa
     7561 atcgtgcgta aactgaatga cgatcgtcag tacaccgccg ccgatggctc tgaaatttct
     7621 ctgcacggac gctcgctgct gtttatccgc aacgtgggtc atttgatgac cattcctgtg
     7681 atttgggaca gcgaaggcaa tgaaatcccg gaaggcattc ttgatggcgt catgactggc
     7741 gcgattgccc tctatgattt aaaagtgcag aaaaactcgc gcactggcag cgtctatatt
     7801 gtgaaaccga aaatgcacgg tccgcaggaa gtggcgttcg ccaacaaact gtttacccgc
     7861 attgagacaa tgctcggtat ggcaccgaat accctgaaaa tgggcattat ggatgaagaa
     7921 cgtcggacct cgctgaactt gcgtagctgt atcgctcagg cacgcaaccg cgtggcgttc
     7981 atcaataccg gtttcctcga ccgtaccggc gatgaaatgc attcggtgat ggaagctggc
     8041 ccgatgctgc gtaaaaatca gatgaaatcg acgccgtgga tcaaagccta cgagcgtaat
     8101 aacgtgcttt ccggcctgtt ctgtgggctg cgcggtaaag cgcaaatcgg taaaggcatg
     8161 tgggcaatgc cggacctgat ggcagacatg tacagccaga agggcgacca actgcgtgcc
     8221 ggggcaaaca cagcctgggt tccgtcgcca actgccgcta cgctccatgc gatgcactac
     8281 caccaaacca acgtacagag cgtgcaagcc aacattgccc agactgagtt caatgctgaa
     8341 tttgaaccgc tgctggacga tctgctgact attccggttg ctgaaaacgc taactggtcg
     8401 gcgcaagaga tccaacaaga gctggataac aacgtgcagg ggattctggg gtacgtggtg
     8461 cgctgggtgg agcaggggat tggttgttca aaagtgccgg atattcacaa tgtggcgctg
     8521 atggaagacc gcgcaacgct gcgtatctcc agccagcata tcgccaactg gttacgtcac
     8581 ggtattctga ccaaagaaca ggtgcaggcg tcgctggaga atatggcgaa agtggttgat
     8641 cagcaaaacg ctgacgatcc ggcttatcgt ccgatggcgg ggaatttcgc taactcgtgt
     8701 gcttttaaag ctgccagcga tttaatcttc ctcggcgtga aacagccaaa cggctatacc
     8761 gaaccgttat tacacgcctg gcgtttacgc gaaaaagaaa gtcattaata gcgtcaaaaa
     8821 aagccccggc aagttttatt gtcggggcta tgatagtaac tgcggtagta catttatccc
     8881 ggaaataacc ggagcgatta tcagggttta acaatattaa atacccgtag cgcattacta
     8941 gcgggcctat aacaacaata tggacatctt aacgatactc tgcgcctgcg tgtgcagaac
     9001 agaaaccctt acccgatata cttgttatgc cagaggtagc aattgcggca accgatctga
     9061 ctggcatgga tgcagatgca atggtggaag atataaatta cacgataatt acagatgttc
     9121 agattaccga acgaactaaa acctatgtca gagcagataa tgctaccact tttcggcagg
     9181 gaacatcagg caccgaaatt caggctagca cagaaaccag caaccaggtt aatctgaaat
     9241 ttgaaggggc taaacctctt ctggaaatac aactgaaata aatatcctcc ggctatgccg
     9301 gaggatattt attttaattt gaagcctgat ttacacgcag ctctgcgtag ggcagggtat
     9361 catagtcagt taacgcattt ttcttatagg ggtttcctat ccagcgagtg atttctttga
     9421 aatgctggtt taatatggaa cgctctggct catagttatc ataacgtaaa ccagccaaat
     9481 ccgaccaggt atggataaaa tcagccagac tgtatttacg gttaatgtac tcagaaaatg
     9541 tatgatgtcg attaagtagc catttttcag aggcccagag aataaatgga atattgtaca
     9601 taggtcgggt tggggtgtct tcgttacgtc ccagcatttt atgtggtggc gtatcgaata
     9661 cttcctctcc atggtcagaa aaatagaaaa gaaaaccatc aggggctgtt gctttgaaag
     9721 ttttgatcaa cgttgcaaca acatggtcat taaatatatt agcattatcg taatcgttat
     9781 ataattccag ttcattatca gtaagtcctt ctggtgcatg ttccctgtcc ccatcgaata
     9841 ctccctgacc ttcaggataa cggtatttat atttgatgtg tgttcccaac aagtgaataa
     9901 taacaaattt tttgggagct ggatctgcca gtacttcact aaacggagcc agtacgttag
     9961 tatcgtactc acgggcactt tgcgttctct gctgattcat gtagtactgt ttatctgttt
    10021 gccgagagaa gactgtcagc attgtgttgc gtgcagtcat cgtttgttga ttggtgatcc
    10081 agaatgtttt gtaaccggcc tgtttcatta tattcatcag ggaaggttgt gtcaggtaga
    10141 gttcgggctg ctgttcatca gcgaaagtca atacctgctg tagtacttcg atggtatatg
    10201 gtcttgagga gacaacatta ttaaacacaa acatattttg ttccgttttg tataattcat
    10261 ctagtccggg ggttgttttt ctgggatatc catacagact cattctccca cgctgggttg
    10321 attctcctat caccagaacc agtgttcgtg gggtatctcc ggaaatatcg ttaaagtttt
    10381 tcagagcggg gagcgcatta ttttcctcca gcaaacggtt cagagaatta agctggagac
    10441 ggtactgata atatccggta ataaattgcc agggggcagc gggctccata cgggatgcca
    10501 ggcgtcccag agtattgtca atagtcctgt ttttaacaac agtatttatt aatacagggt
    10561 gcagaattaa accgtagata ataaataaag gtataatgta gcgccagtat gatggtatat
    10621 atatcgggct aagacgagtc catagcagaa tagcaatagc tgtatatatt gtcgatataa
    10681 atagtatttt taaactaaaa tactgactaa aatattcgct ggcttcactt gtattacttt
    10741 caaacatgac gaacagtaca ctctgagaga attcctgccc gtagattaga taatagcaaa
    10801 gggcagcaag agatgtgccc cataaaacca cgccgattaa tgctgctgta acctttattc
    10861 tctctgggaa aagaaaaaca gggattaacc agagagagct gaaaagtagc gagtcacgaa
    10921 gtccattggt gccactatat ccagtcagac aaatgataat ctgaagtaaa gtggaaaaat
    10981 accagaaata aatgagagcc catcccaggg ctttccagct aaaaacatgt tcatgttttg
    11041 ttaatgtctt ctgcatatta ataaacctgc acgggaattt actattgtaa tttttatatg
    11101 agatgtatta aaacatttca tacagatttt ttgataagca catagtttca atattcacta
    11161 taatcgtttt ctgctgtgtt gtgtttgatc ctttttcaaa gaggcgctaa tcttccactt
    11221 gccaaggtta gatggtcagg gtccgggtta aacaaaatgt ttgacaggga gcctttccat
    11281 atgctgtctt tccagcgtgg cggtccgtaa tgtactaatt ttaagagagg tccgcaatct
    11341 tccagtggat ggttcatctg cctctctatt accgttacca tcgtcttgcg tcctgtgcta
    11401 tcgcctgtaa cgacatcagc gtcactggta ttaacaaact tatagttgaa gccagtcact
    11461 tcgtctcttg gctaacgcag cgtgatgagc tggagtgcca ggcgtgggag aacaacgaca
    11521 gcagcattcg taccggggac gatgacggtt tgctgtgtca gtgcctgggg gatatctgca
    11581 gcaaggcgtt ttcccagacg gggcgcgagt ttgcagagct gggttgccag ttttttctca
    11641 cccagtaaag cgagtttttc gggtgaggga tatcgctgga gaagatcaag tactgcagga
    11701 tgatccagtc gcgggccgag aacgcgctcc agagccggat gtatctgggt caacaagccg
    11761 cggatgcggt tgctggcctg tgttgtctgt gcggcaagat catcatcgaa gccgcaaagc
    11821 atggagagtt cggcgatttg ttcgtcggcc agttttagag agcgaagagc atgaggcaga
    11881 gtacgtgcag cttcggcaat gatggccgca tcacgggcat cggttttggc ttcaccggag
    11941 tgcaaatcgg cgatgcgtcg cattgccagt ccaggaaggt atccgacaag gacgccttca
    12001 gagcgggcaa cggcgacggg taacgccctg atggtagcag gctggtcaac gaccagcagt
    12061 atctgtccat gctgtttcag gtcagatatc agcgctctga gtttgttttc gtcgttgggt
    12121 aatactctgt cgaacaaacg tttacctgaa cgattaacgg caacggcgta atgggtgtct
    12181 ttacctacat caacgccaat aaagaccagg attgattcgt aatcgctgga ttcgggcatt
    12241 gtgtctcccc gtttatacgg gttaaccaga taaccttggg gagcaggtac cggcatccac
    12301 gttacagact gtcccgacat gatgtctgac caggccccta tcagcggtta ccagcgcccc
    12361 accagacccg gtgacatccc cccgaatcat ggacgactgg gggaattaat catgccgggg
    12421 ctggctaaca ccccattaca agggtacgaa taaagtaacg gggagttaca ggcactgaaa
    12481 ctatcatcag agatactgtt gccaaatggc gtaaaggttg gatcccgccg tttgctattg
    12541 ccgcccgact tccttcactg tccctgggaa gctgcggggt ggtgccctgg aaaataatca
    12601 gggggaagaa aattatgcgt cccgatttgt gaaacagaac cggaacagaa aatagcgcag
    12661 caactggcac tcgagttcta ccgtattctg ccccccaaat aaatcacagc taaacagttg
    12721 gttcactcaa gtccacgaaa gcggttcact agaatttcag cgcgtagcca cggggatgga
    12781 gccgatactg cagctatatg tgagacaatt gtcagcgcca gtgatataag acggtaatgg
    12841 agatgcggac gcggccctta attcagagct agccgagtat aaatttctgg acaagtatgt
    12901 aaatgatgag ttaaaaaata atccgtgcat ttccggtgat gatattagtt gttggtaacg
    12961 aaatctcatt agcggaccgg aaacgcactt aacctaaaac attgccaccg ttccgccagg
    13021 agttgcagtg gcaacgtatg ctaccgtcag tgatagcgtt ttgttgttat cagaactgat
    13081 agttcagcga catccagtaa tttcgctcag gtatcacata tcccgttgtt gatgatcccg
    13141 tctggaagta atcaccggca taaagcgtac tcttaccggc actgtacaga ctcacgtcac
    13201 tgtaatcctt gttgagcagg ttattcactg ccgcattcag cgtcagggca tccgtcacct
    13261 tccacgacag acctgcatcc accaccgtcc aggctttcag gtattctcct ttctcatcat
    13321 atactttctt ctgtacagcg ctcagcgacg aataattctg ggtgaaacgt ggtgttttcc
    13381 cgcgataacg ggcacccagc catgatgcca cctcttcggt gatctgccag ttcagtttcg
    13441 cattcaccat gtgttctggg gtataactca gcggtgcacc tttattatca ccatcacgtt
    13501 gttcacttcg ggtccaggtg taattcagcg acagcgtgac atcctctgac catagcggca
    13561 atgtgccggc aaattccaca ccgtgcaacc gggcctttcc gctgtttata tagctattgg
    13621 tgttatcatt tatggaataa gagacaatct tgttagagaa gtcagtcata aaacctgtga
    13681 tattggcgtt cagaccggcg gggttatcgt aatacacccc agcctcataa ctgacgctct
    13741 cttccggctt caggtcgggg ttaccaagta gatttgtttt tccctgcccg gacacaccac
    13801 taatcccttt atggagctgc cccattctgg gtgccttata tcccgtggtc acaccgcctt
    13861 tcagcgtcca ggcatctgcc acatcccaga ccagatatgc acgcggactg aagtgccccc
    13921 cgaattgctc atgatgttca tagcggctgc ccgcagtcag cgcaagtgca tccgtgagat
    13981 gccactcgtc ctcagcaaat accgaccagc ttttctgccg gaaagtttca cctgtgctgg
    14041 caaggacaac cccatctttc atggacgaac tctgatactc gccccccaca gtaaccagat
    14101 gagattctcc cagaggggta agcagcaatg aattcaggat aaggttagat tccttaagct
    14161 cccgcagttg accggcaagg ccccatttgt cgcgtttcag tacattgcgt acaagctcac
    14221 gacctttatt ttc
//
LOCUS       DAZFWL020000123        14070 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFWL020000123 DAZFWL020000000
VERSION     DAZFWL020000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.123"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            254..553
                     /gene="yoaC"
                     /locus_tag="ACHKNP_RS19170"
                     /old_locus_tag="pgaptmp_003834"
     CDS             254..553
                     /gene="yoaC"
                     /locus_tag="ACHKNP_RS19170"
                     /old_locus_tag="pgaptmp_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HGU4705921.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(673..852)
                     /gene="yoaH"
                     /locus_tag="ACHKNP_RS19175"
                     /old_locus_tag="pgaptmp_003835"
     CDS             complement(673..852)
                     /gene="yoaH"
                     /locus_tag="ACHKNP_RS19175"
                     /old_locus_tag="pgaptmp_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HGU4705922.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            926..2287
                     /gene="pabB"
                     /locus_tag="ACHKNP_RS19180"
                     /old_locus_tag="pgaptmp_003836"
     CDS             926..2287
                     /gene="pabB"
                     /locus_tag="ACHKNP_RS19180"
                     /old_locus_tag="pgaptmp_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HGU4705923.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2291..2869
                     /gene="nudL"
                     /locus_tag="ACHKNP_RS19185"
                     /old_locus_tag="pgaptmp_003837"
     CDS             2291..2869
                     /gene="nudL"
                     /locus_tag="ACHKNP_RS19185"
                     /old_locus_tag="pgaptmp_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HGU4705924.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3053..4417
                     /gene="sdaA"
                     /locus_tag="ACHKNP_RS19190"
                     /old_locus_tag="pgaptmp_003838"
     CDS             3053..4417
                     /gene="sdaA"
                     /locus_tag="ACHKNP_RS19190"
                     /old_locus_tag="pgaptmp_003838"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU4705925.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4548..6146
                     /gene="pdeD"
                     /locus_tag="ACHKNP_RS19195"
                     /old_locus_tag="pgaptmp_003839"
     CDS             4548..6146
                     /gene="pdeD"
                     /locus_tag="ACHKNP_RS19195"
                     /old_locus_tag="pgaptmp_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4705926.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6150..7706)
                     /gene="yoaE"
                     /locus_tag="ACHKNP_RS19200"
                     /old_locus_tag="pgaptmp_003840"
     CDS             complement(6150..7706)
                     /gene="yoaE"
                     /locus_tag="ACHKNP_RS19200"
                     /old_locus_tag="pgaptmp_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HGU4705927.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7694..7852)
                     /gene="yoaL"
                     /locus_tag="ACHKNP_RS19205"
                     /old_locus_tag="pgaptmp_003841"
     CDS             complement(7694..7852)
                     /gene="yoaL"
                     /locus_tag="ACHKNP_RS19205"
                     /old_locus_tag="pgaptmp_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HGU4705928.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8169..9140
                     /gene="manX"
                     /locus_tag="ACHKNP_RS19210"
                     /old_locus_tag="pgaptmp_003842"
     CDS             8169..9140
                     /gene="manX"
                     /locus_tag="ACHKNP_RS19210"
                     /old_locus_tag="pgaptmp_003842"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HGU4705929.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAQAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9203..10003
                     /gene="manY"
                     /locus_tag="ACHKNP_RS19215"
                     /old_locus_tag="pgaptmp_003843"
     CDS             9203..10003
                     /gene="manY"
                     /locus_tag="ACHKNP_RS19215"
                     /old_locus_tag="pgaptmp_003843"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HGU4705930.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10016..10867
                     /gene="manZ"
                     /locus_tag="ACHKNP_RS19220"
                     /old_locus_tag="pgaptmp_003844"
     CDS             10016..10867
                     /gene="manZ"
                     /locus_tag="ACHKNP_RS19220"
                     /old_locus_tag="pgaptmp_003844"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HGU4705931.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10922..11380
                     /gene="yobD"
                     /locus_tag="ACHKNP_RS19225"
                     /old_locus_tag="pgaptmp_003845"
     CDS             10922..11380
                     /gene="yobD"
                     /locus_tag="ACHKNP_RS19225"
                     /old_locus_tag="pgaptmp_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HGU4705932.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            11809..12375
                     /gene="mntP"
                     /locus_tag="ACHKNP_RS19230"
                     /old_locus_tag="pgaptmp_003846"
     CDS             11809..12375
                     /gene="mntP"
                     /locus_tag="ACHKNP_RS19230"
                     /old_locus_tag="pgaptmp_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HGU4705933.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(12372..13181)
                     /gene="rlmA"
                     /locus_tag="ACHKNP_RS19235"
                     /old_locus_tag="pgaptmp_003847"
     CDS             complement(12372..13181)
                     /gene="rlmA"
                     /locus_tag="ACHKNP_RS19235"
                     /old_locus_tag="pgaptmp_003847"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HGU4705934.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(13347..13556)
                     /gene="cspE"
                     /locus_tag="ACHKNP_RS19240"
                     /old_locus_tag="pgaptmp_003848"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(13347..13556)
                     /gene="cspE"
                     /locus_tag="ACHKNP_RS19240"
                     /old_locus_tag="pgaptmp_003848"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU4705935.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(13569..13712)
                     /gene="yobF"
                     /locus_tag="ACHKNP_RS19245"
                     /old_locus_tag="pgaptmp_003849"
     CDS             complement(13569..13712)
                     /gene="yobF"
                     /locus_tag="ACHKNP_RS19245"
                     /old_locus_tag="pgaptmp_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HGU4705936.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT         3482 a         3506 c         3485 g         3597 t
ORIGIN      
        1 catgccgttg tgttcgccct ctgactagca actctggttt tctttcttag ctgaataatc
       61 tggttatagc gtgaggactg accgttaaat ttctccatct tactggataa cacaccatga
      121 gctgctacct ttaaaacacc tgaatgtcaa caggttaact cgtgccatat cgttataatc
      181 cctttgatat tagatgcaaa ttaaggtcat atatagtctt atcgataaca tggttaattt
      241 taaggataag aatatgcctg ctgtaataga taaagccctg gatttcattg gtgccatgga
      301 tgtatcagcg ccaacaccaa gttcgatgaa tgaaagcacg gcgaagggaa tctttaaata
      361 tttaaaagaa ctgggagtac ccgcgagtgc cgctgatatt acggcgcgag ctgaccagga
      421 aggctggaac cctgggttca cggaaaaaat ggttggatgg gcaaaaaaaa tggagacagg
      481 tgaacgctct gtgattaaaa atcctgaata cttttcaaca tatatgcagg aagaactaaa
      541 agcactggtc tgagttaaat ttatatcagc ataaatgggt caggacgctt ttaatcacat
      601 ataaaaagcg tcctttttct cacaatcaaa tgatcatcgg cttctataac gaaatcgata
      661 cggattagtg tgttactcgt cttcatcctc aaaacgcgcc acgattcgct cgccggagtg
      721 gttggcacgc agctcttctg ccaccagcgc gattgcctgg ccgctgctca tcccttgtgc
      781 catcagttcc tggatccgct cgacagcttt ttgctgctgt tcatgggtga gtgaaggtaa
      841 acctgcaaac attgttaact cctgctaaat tgttggcgct aattatttca tgctacccgg
      901 cacatagcca gtagagtcag gactgatgaa gacgttatct cccgctgtga ttactttacc
      961 ctggcgtcag gacgccgctg aattttattt ctcccgctta agccacctgc cgtgggcgat
     1021 gcttttacac tccggctatg ccgatcatcc gtatagccgc tttgatattg tggtcgccga
     1081 tccgatttgc actttaacca ctttcggtaa agaaaccgtt gttagtgaaa gcgaaaaacg
     1141 cacaacgacc actgatgacc cgctacaggt gctccagcag gtgctggatc gcgcagacat
     1201 tcgcccaacg cataacgaag atttgccatt tcagggcggc gcactggggt tgtttggcta
     1261 cgatctgggc cgccgttttg agtcactgcc agaaattgcg gaacaagata tcgttctgcc
     1321 ggatatggca gtgggtatct acgattgggc gctcattgtc gaccaccagc gtcatacagt
     1381 ttctttgctg agtcataatg atgtcaatgc ccgtcgggcc tggctggaaa gccagcaatt
     1441 ctcgccgcag gaagatttca cgctcacttc cgactggcaa tccaatatga cccgcgagca
     1501 gtacggcgaa aaatttcgcc aggtacagga atatctgcac agcggtgatt gctatcaggt
     1561 gaatctcgcc cagcgttttc atgcgaccta ttctggcgat gaatggcagg cattccttca
     1621 gcttaatcag gccaaccgcg cgccatttag cgctttttta cgtcttgaac agggtgcaat
     1681 tttaagcctt tcgccagagc ggtttattct ttgtgataat agtgaaatcc agacccgccc
     1741 gattaaaggc acgctaccac gcctgcccga tcctcaggaa gatagcaaac aagcagaaaa
     1801 actggcgaac tcagcgaaag atcgtgccga aaatctgatg attgtcgatt taatgcgtaa
     1861 tgatatcggt cgtgttgccg tagccggttc ggtaaaagta ccagagctct tcgtggtgga
     1921 acccttccct gccgtgcatc atctggtcag cactataacg gcgcgactac cagaacagtt
     1981 acacgccagc gatctgctgc gcgcagcttt tcctggtggc tcaataaccg gggctccgaa
     2041 agtacgggct atggaaatta tcgacgaact ggaaccgcag cgacgtaatg cctggtgcgg
     2101 cagcattggc tatttgagct tttgcggcaa catggatacc agcattacta tccgcacgct
     2161 gactgccatt aacggacaaa tatactgctc tgcgggcggt ggaattgtcg ccgatagcca
     2221 ggaagaagcg gaatatcagg aaacttttga taaagttaat aagatattac gccaactgga
     2281 gaagtaagac gtggaatacc gtagcctgac gcttgatgat tttttatctc gctttcaact
     2341 tttgcgcccg caaattaacc gggaaaccct aaatcatcgt caggctgccg tgttaatccc
     2401 catcgtccgt cgaccgcaac cggggttgtt gctgactcag cgttcaattc atctgcgtaa
     2461 acacgctgga caagtggcat tccctggagg tgcagtcgat gacacggacg catcagttat
     2521 cgccgccgcg ctgcgcgaag ctgaagaaga ggtcgctata ccgccttccg ccgttgaagt
     2581 tatcggcgtg ctgccgcccg tcgatagcgt aactggctat caggtaaccc cagtggtcgg
     2641 cattatcccg cccgatctgc cgtatcgcgc cagtgaagat gaagtctcgg cggtgtttga
     2701 aatgccgctc gcccaggcat tacatctggg tcgttatcac cctttagata tctaccgccg
     2761 tggcgattca catcgggtat ggctgtcctg gtacgaacag tattttgtat ggggaatgac
     2821 cgcaggcata attcgtgagc tggcgctgca aattggtgtg aaaccctgac tatacttatc
     2881 tttacatcta caaaacacta cttgagacaa tcatcgcaat attagttaaa tcgcggtttt
     2941 tgattagttt aattcatgtg aatagttaag ccagtcgccg cgttccctct tacactatgc
     3001 gctgttatta gttcgttact ggaagtccag tcaccttgtc aggagtatta tcgtgattag
     3061 tctattcgac atgtttaagg tggggattgg tccctcatct tcccataccg tagggcctat
     3121 gaaggcgggt aaacagttcg tcgatgatct ggtcgaaaaa ggcttactgg atagcgttac
     3181 tcgcgttgcc gtggacgttt atggttcact gtcgctgacg ggtaaaggcc accacaccga
     3241 tatcgccatt attatgggtc ttgcaggtaa cgaacctgcc accgtggata tcgacagtat
     3301 tcccggtttt attcgcgacg tagaagagcg cgaacgtctg ctgctggcac agggacggca
     3361 tgaagtggat ttcccgcgcg acaacgggat gcgttttcat aacggcaacc tgccgctgca
     3421 tgaaaacggt atgcaaatcc acgcctataa cggcgatgaa gtcgtctaca gcaaaactta
     3481 ttattccatc ggcggcggtt ttatcgtcga tgaagaacac tttggtcagg atgctgccaa
     3541 cgaagtgagc gtgccgtatc cgttcaaatc tgccaccgaa ctgctcgcgt actgtaatga
     3601 aaccggctat tcgctgtctg gtctcgctat gcagaacgaa ctggcgctgc acagcaagaa
     3661 agagatcgac gagtatttcg cgcatgtctg gcaaaccatg caggcatgta tcgatcgcgg
     3721 gatgaacacc gaaggtgtac tgccaggccc gctgcgcgtg ccacgtcgtg cgtctgccct
     3781 gcgccggatg ttagtttcca gcgataaact gtctaacgat ccgatgaatg tcattgactg
     3841 ggtaaacatg tttgcgctgg cagttaacga agaaaacgcc gccggtggtc gtgtggtaac
     3901 tgcgccaacc aacggtgcct gcggtatcgt tccggcagtg ctggcttact atgaccactt
     3961 tattgaatcg gtcagcccgg acatctatac ccgttacttt atggcagcgg gcgcgattgg
     4021 tgcattgtat aaaatgaacg cctctatttc cggtgcggaa gttggttgcc agggcgaagt
     4081 gggtgttgcc tgttcaatgg ctgctgcggg tcttgcagaa ctgctgggcg gtagcccgga
     4141 acaggtttgc gtggcggcgg aaattggcat ggaacacaac cttggtttaa cctgcgaccc
     4201 ggttgcaggg caggttcagg tgccgtgcat tgagcgtaat gccattgcct ctgtgaaggc
     4261 gattaacgcc gcacggatgg ctctgcgccg caccagtgca ccgcgcgtct cgctggataa
     4321 ggtcatcgaa acgatgtacg aaaccggtaa ggacatgaac gccaaatacc gcgaaacctc
     4381 acgcggtggt ctggcaatca aagtccagtg tgactaatac ttcttactcg cccatctgca
     4441 acggatgggc gaatttatac ccgctttctc gtctgctgta atattcccca ctacacttcc
     4501 actgttgcgt caggcgtttg tcgccatacg cttacagggt ggcccgcatg caaaaagcac
     4561 aacggatcat taaaacctat cgccgtaatc gaatgattgt ttgtacgatt tgcgcactcg
     4621 ttacgctcgc ttcgaccctg agcgtgcgat ttatttcaca gcgtaactta aatcaacaac
     4681 gggtagtaca attcgccaat cacgctgtag aggaattaga taaagtactg cttcccctac
     4741 aggcaggtag cgaagtcttg cttccgctga ttggtctgcc ctgctctgtc gcccatttgc
     4801 cattacgtaa acaggcggca aaactccaaa ctgtgcgatc cattggcctg gtgcaagacg
     4861 gcacactcta ttgctccagc atttttggtt atcgcaatgt gcccgtcgtg gacattctgg
     4921 ctgaacttcc tgcaccgcaa ccacttttac gcctgacgat cgaccgtgcc ctgattaaag
     4981 gcagtccggt tttgattcaa tggacgcctg cagcgggcag tagcaatgct ggggtcatgg
     5041 agatgattaa catcgactta ctgacggcaa tgctccttga gccacaactg ccgcaaatca
     5101 gtagcgccag cctgacggtg gacaaacggc atttgctcta tggtaatggg ctggtagatt
     5161 cccttccgca acctgaagac aatgaaaact atcaggtttc ttcgcaacgc tttcctttta
     5221 ccattaacgt taatggtccg ggggctacgg cgctggcatg gcactatctt ccaacacaat
     5281 taccgctggc ggtgctgcta agtttactgg tgggctacat cgcctggctg gcgaccgctt
     5341 accggatgag cttttcccgc gaaatcaatc tgggcctggc gcaacatgag ttcgaattgt
     5401 tctgtcagcc tttgcttaat gcgcgcagcc agcaatgtat tggtgtagag attttgctgc
     5461 gctggaataa tccgcgtcag ggctggattt caccggatgt gtttattcct atcgcggaag
     5521 aacatcattt aattgtgcca ctgacccgct atgtgatggc agaaaccatt cgtcagcgcc
     5581 atgttttccc gatgagtagt cagtttcatg ttggcattaa cgtcgcaccc agccattttc
     5641 gccgtggtgt gctgataaaa gatctcaatc agtactggtt tagcgctcac ccgattcagc
     5701 aactgatcct cgaaatcacc gaacgcgatg ccttactgga tgttgattat cggattgccc
     5761 gcgagctaca tcgtaaaaac gtcaaactgg cgattgatga cttcggcacc ggcaacagtt
     5821 cattttcctg gcttgaaaca ttacgtcctg acgtgctgaa aattgataag tcatttaccg
     5881 cagctatagg ttctgacgcg gttaactcga cggtgaccga tatcatcatc gcgctggggc
     5941 aaagactgaa tattgaactg gtggcggagg gcgtggaaac gcaagaacag gcgaagtatt
     6001 tgcgccgtca tggcgtgcat attttgcaag ggtatttgta cgcacagccg atgccgctac
     6061 gtgattttcc caaatggctg gcgggcagcc aaccgccgcc cgcccggcat aatggacata
     6121 tcacgcccat tatgccgtta cgttaatgct tactcatctt catcgtgcgc cggttgctct
     6181 ttaacaatgc gaaccagatc aacacgataa tcattggctt caatgatggt gatatgcagt
     6241 ggccctacat caatcacatc gcccacacgg ggaatgtgac catttgccga gatcacgagg
     6301 cccgcgaccg tcgcgatatc atcgtcatcg gcaaggtgct caacatcaag cgcctgctgc
     6361 aaggcatgca aatctgtacc gccttttacc agccagccgt caccatcagt aatgatttcc
     6421 ggcgtttcgt cagcgtccgg gaattcaccc gcaatggctt ccagcacatc cagcggcgtg
     6481 accagacctt gtaccacacc aaactcgttg gtcacgataa caaagctccc gcgagcacga
     6541 cgcagcacgc ccaacaggtt gatcggatcg agggtttccg ggacgataat cgccggagac
     6601 gccgaagcaa tcgccgccac atcaacgccc tcttccagcg ccaccagcag ttctttagca
     6661 cgtacaatac cgatgatttc atccagttca ccgcgacata ccgggaacag actgtgcggt
     6721 gaagagagca gttgctcgcg gatttcatcg accccgagat tagcgtcaac ccagcttatt
     6781 tcaccgcgcg gcgtcatgat cccgcgcaga gaacgcgacg ccagcgtcag tacgccgtta
     6841 atcatgtaac gttcttcttc ggcaaatgca ccttccggga tcggcatcgg catcgggtta
     6901 tcggcatcgt gctgaacatt ggcctgacgt ttcccgccca tcaaacgcag gatggcatcg
     6961 gcagtacgcg ctcgcagcgg caaagtcgac tggtggcgaa taaagttgcg acgcgcaatc
     7021 tggttaaaca cttcgatgat gatcgagaag ccaatcgcgg catacaggta acctttcgga
     7081 atgtggaaac cgaaaccttc tgccaccaga ctcagaccaa tcattaacag gaagctcaga
     7141 cagagcacca ccaccgtggg gtgctggtta acgaatcgcg tcagcggttt ggatgccagc
     7201 aacataaccg ccatcgcaat cactaccgcc gccatcatca ccggcagatg gttaaccatc
     7261 cctactgcag taattaccgc atccaacgag aagacggcgt caaggatgac aatctgtgtg
     7321 acgaccaccc agaaactggc gtagccttta ccgtggccgg aatcatgatc gcggttttcc
     7381 agccgttcat gcagttcggt tgttgctttg aacagcaaga atatcccccc gaacaacata
     7441 atcaggtcgc gtccggagaa ggagaaatcc atgacggtaa atagcggttt ggtcagcgtg
     7501 accatccatg aaatcagcga cagcagcccc agacgcataa tcagcgccag tgataacccc
     7561 agcaaacgcg ctttatcgcg ttgttttggc ggcagtttgt cagcaagaat ggcgatgaag
     7621 accaggttat cgatacccag cacaatttcg agaacaacaa gcgtgagtag ccccgcccaa
     7681 attgaggggt ccattaagaa ttccatgaca agctcctgct taaggaatag ctattcgacg
     7741 ccagaaataa tgcaggcgta acgacaaaat gcaaacgaaa ggtgcggcat agagagccag
     7801 aaaggtaggc gttaaaaggc ctgatgctga aatgacgtcg gtgacgatcc atactgcggg
     7861 ctactgccct atactccatg gttgttaaac gggagttaaa catatcagag acgcctctga
     7921 tttggcaaag atttaccttc ctttgcaaac gaatgtgaca aggatatttt acctttcgaa
     7981 atttctgcta atcgaaagtt aaattacgga tcttcatcac ataaaataat tttttcgata
     8041 tctaaaataa atcgcgaaac gcaggggttt ttggttgtag cccttatctg aatcgattcg
     8101 attgtggacg acgattcaaa aatacatctg gcacgttgag gtgttaacga taataaagga
     8161 ggtagcaagt gaccattgct attgttatag gcacacatgg ttgggctgca gagcagttgc
     8221 ttaaaacggc agaaatgctg ttaggcgagc aggaaaacgt cggctggatc gatttcgttc
     8281 caggtgaaaa tgccgaaacg ctgattgaaa agtacaacgc tcagttggca aaactcgaca
     8341 ccactaaagg cgtgctgttt ctcgttgata catggggagg cagcccgttc aatgctgcca
     8401 gccgcattgt cgtcgacaaa gagcattatg aagtcattgc aggcgttaac attccaatgc
     8461 tcgtggaaac gttaatggcc cgtgatgatg acccaagctt tgatgaactg gtggcactgg
     8521 cagtagaaac aggccgtgaa ggcgtgaaag ctctgaaagc caaaccggtt gaaaaagccg
     8581 cgcaagcacc cgctgccgca gcaccaaaag cggctccaac tccggcaaaa ccaatggggc
     8641 caaacgacta catggttatt ggccttgcgc gtatcgacga ccgtctgatt cacggtcagg
     8701 tcgccacccg ctggaccaaa gaaaccaatg tctcccgtat tattgttgtt agtgatgaag
     8761 tggctgcgga taccgttcgt aagacactgc tcacccaggt tgcacctccg ggcgtaacag
     8821 cacacgtagt tgatgttgcc aaaatgattc gcgtctacaa caacccgaaa tatgctggcg
     8881 aacgcgtaat gctgttattt accaacccaa cagatgtaga gcgtctcgtt gaaggcggcg
     8941 tgaaaatcac ctctgttaac gtcggtggta tggcattccg tcagggtaaa acccaggtga
     9001 ataacgcggt ttcggttgat gaaaaagata tcgaggcgtt caagaaactg aatgcgcgcg
     9061 gtattgagct ggaagtccgt aaggtttcca ccgatccgaa actgaaaatg atggatctga
     9121 tcagcaaaat cgataagtaa cgtattgtgt tgattatcac tcagttttca cacttaagtc
     9181 ttacgtaaac aggagaagta caatggagat taccactctt caaattgtgc tggtatttat
     9241 cgtagcctgt atcgcaggta tgggatcaat cctcgatgaa tttcagtttc accgtccgct
     9301 aatcgcgtgt accctggtgg gtatcgttct tggggatatg aaaaccggta ttattatcgg
     9361 tggtacgctg gaaatgatcg cgctgggctg gatgaacatc ggtgctgcag ttgcgcctga
     9421 cgccgctctg gcttctatca tttctaccat tctggttatc gcaggtcatc agagcattgg
     9481 tgcaggtatc gcactggcaa tccctctggc cgctgcgggc caggtactga ccatcatcgt
     9541 tcgtactatt accgttgctt tccagcacgc tgcggataag gctgctgata acggcaacct
     9601 gacagcgatt tcctggatcc acgtttcttc tctgttcctg caagcaatgc gtgtggctat
     9661 tccggccgtc atcgttgcgc tgtctgttgg taccagcgaa gtacagaaca tgctgaatgc
     9721 gattccggaa gtggtgacca atggtctgaa tatcgccggt ggcatgatcg tggtggttgg
     9781 ttatgcgatg gttatcaaca tgatgcgtgc tggctacctg atgccgttct tctacctcgg
     9841 cttcgtaacc gcagcattca ccaactttaa cctggttgct ctgggtgtga ttggtactgt
     9901 tatggcagtg ctctacatcc aacttagccc gaaatacaac cgcgtagccg gtgcgcctgc
     9961 tcaggcagct ggtaacaacg atctcgataa cgaactggac taacaggtga gcgaaatggt
    10021 tgatacaact caaactacca ccgagaaaaa actcactcaa agtgatattc gtggcgtctt
    10081 cctgcgttct aacctcttcc agggttcatg gaacttcgaa cgtatgcagg cactgggttt
    10141 ctgcttctct atggtaccgg caattcgtcg cctctaccct gagaacaacg aagctcgtaa
    10201 acaagctatt cgccgtcacc tggagttctt taacacccag ccgttcgtgg ctgcgccgat
    10261 tctcggcgta accctggcgc tggaagaaca gcgtgctaat ggcgcagaga tcgacgacgg
    10321 tgctatcaac ggtatcaaag tcggtttgat ggggccgctg gctggtgtag gcgacccgat
    10381 cttctgggga accgtacgtc cggtatttgc agcactgggt gccggtatcg cgatgagcgg
    10441 cagcctgtta ggtccgctgc tgttcttcat cctgtttaac ctggtgcgtc tggcaacccg
    10501 ttactacggc gtagcgtatg gttactccaa aggtatcgat atcgttaaag atatgggtgg
    10561 tggcttcctg caaaaactga cggaaggggc gtctatcctc ggcctgtttg tcatgggggc
    10621 attggttaac aagtggacac atgtcaacat cccgctggtt gtctctcgca ttactgacca
    10681 gacgggcaaa gaacacgtta ctactgtcca gactattctg gaccagttaa tgccaggcct
    10741 ggtaccactg ctgctgacct ttgcttgtat gtggctactg cgcaaaaaag ttaacccgct
    10801 gtggatcatc gttggcttct tcgtcatcgg tatcgctggt tacgcttgcg gcctgctggg
    10861 actgtaagac tgttgtacac taccggggcc ttttggcccc gtttttttat ctggaggatt
    10921 aatgacaatc acggacctgg tactgattct tttcatcgca gcactcctgg ccttcgcgat
    10981 ctacgatcag ttcatcatgc cccgccgtaa cggccccacc ctgctggcaa ttcctttgct
    11041 ccggcgtggt cgcatcgata gcgttatctt cgtcggattg attgtcattc ttatctataa
    11101 caacgtcacg aatcatggtg cgttaataac gacatggtta ttaagcgcac tggctctgat
    11161 gggtttttat atattctgga tccgcgttcc gaagatcatc tttaaacaaa aaggtttttt
    11221 cttcgccaat gtctggattg aatatagccg aatcaaagcg atgaacttgt cggaagatgg
    11281 cgtgttggtg atgcaattag aacagcgtcg gctgttaatc cgcgttcgaa atatcgacga
    11341 tctggaaaaa atttataagc ttctcgtttc aactcaataa gttatgaatt tagccaaagc
    11401 tatgtttagt gtatttttaa taatcagaca tagcttaggc tatattacct cttcccttat
    11461 ttgttattta ttttaacgtt tcattgatat ataaatctaa atgtaaatcg ttatcaataa
    11521 agcaatgaaa taatatattc caacagttgt tttatattct caaaatatgt taaggttgcg
    11581 ccctcatttg gggagtagcc gatttccaga ttccggaaat gtacgtgtca acatactcgt
    11641 tgcaaaacgt ggcacgtacg gactgaatac tttcagtcag gcgagaccat atgcacatca
    11701 atcgctatgc ctgcatgaac gcgaaaacca tcgcggcttg ttgttcatgt atgcatgttt
    11761 gctgggggcg atgatgtgtt ttatggatac cccggtcagg acattgtcat gaatatcact
    11821 gctactgttc ttcttgcgtt tggtatgtcg atggatgcat ttgctgcatc aatcggtaaa
    11881 ggtgccaccc tccataaacc gaaattttct gaagcattgc gaaccggcct tatttttggt
    11941 gccgtcgaaa ccctgacgcc gctgatcggc tggggaatgg gcatgttagc cagccggttt
    12001 gtccttgaat ggaaccactg gattgcgttt gtgctgctga tattcctcgg cgggcgaatg
    12061 attattgagg gttttcgtgg cgcagatgat gaagatgaag agccgcgccg tcggcacggt
    12121 ttctggctac tggtaaccac cgcgattgcc accagcctgg atgccatggc tgtgggtgtt
    12181 ggtcttgctt tcctgcaggt caacattatc gcgaccgcat tggccattgg ttgtgcaacc
    12241 ttgattatgt cgacattagg gatgatggtt ggtcgcttta tcggctcaat tattgggaaa
    12301 aaagcggaaa ttctcggcgg gctggtgctg atcggcatcg gcgtccagat cctctggacg
    12361 cacttccacg gttaataaga acgctgccag aggtgaatat taaagtccgt ctggcagtcg
    12421 aacacttctt ttgctgccag tgtttgccag acttctggct tcgcacgcca ggcaaacggc
    12481 gtcatctgca ataatgcgac ggcttcatca ccgcgaagac gcatcggata acacaactca
    12541 tcactctgct gtaatgtaaa accttccagt tgttctgcat gaggtgcatg aagatgtact
    12601 tcattgtaaa tcagcccctt cagctccatc aaatgtcgcg gtcccggcgt ggcagtaatg
    12661 acccagccgc cgggcttcac tactcgtgct aattcttctg ctttacacgg cgcgtaaata
    12721 cgtattatgg cgtccatact ggtatcggaa aacggcaaac ggtggctgga agcgacacaa
    12781 agagtgacct gcggatagcg tttcgccgcc gcttttatcg ctaccttcga gacatccagg
    12841 ccaaacgtgg tgatttcggg caacacatcg gcaaatgcgt gtgtgtaata cccttcacca
    12901 cagccaatat ccagcaccgc cgtggccttt tcatcaagcc gttcccgcag ttgggcgaca
    12961 attgcatcac gcagcggctg ataatgtccg gcatctaaga atgcgcggcg tgcttgtatc
    13021 atttccgcgc tgtcgcccgg atcacgagac cgtttatgct gaacgggcag cagattgaca
    13081 tacccttctt tcgccatatc aaactgatgt cgctggggac agatatagct gtttttttca
    13141 cgcgaaagag gctgatggca aagtggacag gaaaacgaca tgacaactcc ggcaggatac
    13201 taaaggccgc aagtgtaacg cgaatcacgc ggcgggcaaa tatccgaacg ccaggagcga
    13261 agataaagca tagtaaaaag cctcgcattc gacgaggctt tatagcgaga ttgaagcgta
    13321 ttcacacttc agatcagtgg attcgatcag atagctgtta cgttaacagc tgccggacct
    13381 ttctggccgt cctgaatttc gaactcaacg ttctgacctt cagccagagt tttgaagcca
    13441 ttaccctgga tagcggagaa gtgtacgaaa acatctttgc tgccatcagc cggagtaatg
    13501 aagccaaaac ctttagactc gttgaaccac ttaacctgac ctttaatctt tgccatttga
    13561 aaaattcctt agattgtttt cttcgcccgc aggcataaca tagataaaac tgacacatta
    13621 ctgcatgagg caccaatata aggctcggca gagaagcggt attcaacgtc aacgtgttta
    13681 ctcaggactt ctttactgaa aatgccacac ataaacagaa ctgtacctcg tttaacccga
    13741 aatctgttat cacatacaac gttaattatg gcaagccatt tttaaacatg tctcgatcag
    13801 acgcacaaat ctcgatacgt cttccacttt ttttgcacac ttatgcaacg gaatacgcgc
    13861 cgatatatca ttgtgcttaa ccttgccagt tcaggcagat acttaaactg gcgtattttc
    13921 taacatagtt caataagttc agttgttcca ggccagtggg agaagttatt acatagtgcg
    13981 tgcaatatca cattttttgc tatgcaatga ataaaaagtt atatcacttt ttctcataaa
    14041 acagtcagtt aacggctatc aattacccta
//
LOCUS       DAZFWL020000124        13746 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFWL020000124 DAZFWL020000000
VERSION     DAZFWL020000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.124"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(101..7072)
                     /locus_tag="ACHKNP_RS19250"
                     /old_locus_tag="pgaptmp_003850"
     CDS             complement(101..7072)
                     /locus_tag="ACHKNP_RS19250"
                     /old_locus_tag="pgaptmp_003850"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 25 domain-containing
                     protein"
                     /protein_id="HGU4705937.1"
                     /translation="MESAGRLDISAGSLNNHQGTVVSDGLSVTLDGALDNTSGRLLSQ
                     KTLSVSGSELVSDDGLIQSGSDMTLDVQDGVLSNRNTKTRGGISSAGTLTVRAGMLNN
                     QQGFMVGQKDMTLNAGTLDNRQGVLGSQASLQISSGTLMNQKGALKAGTDMLLSGGDV
                     SNQEGTLAAGGDLNTHLNVLENQQGTVVSNGNSRLDVTRFDNQGGRLVAQQSLTLSST
                     DIINDASGLIQSGASLNLRADTLSNRNSGDRGGVISQGAMTLNAGTLDSTAGVLLSGD
                     ALSLTAGVVNNTSGQVVANGLLGWNSQALNNQSGLIQGKGISINTAGQTLDNRGGTLN
                     SLQELTVSTGAMDNRGGTVGAKTTADLSTTSLDNREGGRLVSEGELRLHTGGLQNSHG
                     QIQSVGDILFDSVRGVVDNVSGLIRSGSAITLNALQFINRHTQNTGQGLEAQTIHITT
                     QDLDNQEGSILADRALTVMADRTLSNNDGVLSSGATLSVSGRQLAFSNRDGVVKAGQS
                     VSVDAGQLGGDGKLLSPGDITLKSNTTFSNSGQTIANGNLTLSVNGDVSNTGSLLAGS
                     RLDLNSIRLENTEKGEISAGQTWLNVTDTLLNRGLIDGKYTRLQVNTLTNSGTGRIYG
                     DAVGVSAATLNNLDENGVAATLAGRERVDLGVQTLNNRTHSLIYSAGDMHIGGTLDAN
                     GAATGKAGVLNNHSATIEAAGYLALSAGQINNVNDHFTTERVVVSTEKVTEYQLSGSD
                     KRWSAGEPGVYVDNDSSNSLKKLHTPEGARDKFTQYDYTRTVEETRVKDSDPGKILSG
                     AGMTIVADKLLNDKSQVVAGGLLTIPSGSVENVSVSGERHVTDSGTSTYYYRIRKKGK
                     DKQGEKTSQYTPPTVIQSITLKPGELTSHGQIQGSQVTLSPLKPQGTDVQTGQAGNVD
                     AAVAATDRIPLRPVVSAGEPVILLPGQQFEVSTPQGSIHVAGPDTRLPDSSLFKTNPA
                     VNVPYLVETDPRFTNQKTWLGSDYMQKAFSQNGDNMLKRLGDGFYEQRLIREQVVALT
                     GQRYLDGYSNDEEQFKALMDAGIAFGKQYNLTPGVALTAEQMALLTGDIVWLVNTTVT
                     LPDGSTQTVQVPQVYARVKPGDVNSAGALIAGRDMVMKLDGDLFNSGKLAGKQTVQLS
                     AENIHNQAGTIQGANVSLTAWTDINSTGGLLQATDSLLAMAGRDISLTTTTRTAQSDA
                     GQNHFERTSIDSVAGVYVQNDQGRLVLQAGRDMNLTAATVVNQGKDSLTQLSAGGDMT
                     LSTVTTSAQDNITWDKNNRLSQGVTQSTGSTLAGNGDVTLTAGRDMTSQAASLSAQKG
                     LALMAGHDVTLTGAQNTSSLDEYHKVTGSSGMLSKTTTTTHDVTDRRTMTGSELNGDT
                     VSIGAGHNLNVTGSSVAGDNRVSLAAGNNLNIGTLAESNRETHLKQEKKSGLMSSGGV
                     GFSVGSQSLKVTDTATDTTQKGSTVGSVHGDVSLQAGNRLTVNGSDLIAGRDMALSGK
                     EVSITAATDQHVQTHTVEQKTSGLTLALSGTAGSALNTTVETVQAAKSAGNSRLEALQ
                     GVKAALSGAQAVQAGRLADAQGADAENNNTVGISLSYGSQSSKSEQQSEQTVAKGSTL
                     TAGNHLSIQATGSGVKGVDGDLTIQGSQIKAGNNVLLQANRDVNLVSAENTSKLEGKN
                     TSSGGSVGVGVGVGSGGWGISVSASANQGKGSEKGNGTTHTETMVDAGNRLTIISGRD
                     TTLTGAQAGGETVKVDAGRHLTLTSEQDSDRYDSKQQNASAGGSFTFGSMSGSASVNL
                     SRDKMHSNYDSVQEQTGIFAGRGGFDVTTGQHTQLNGAVIASTATADKNRLDTGTLGF
                     SDIENRADFKTEHQSAGLSTGGSVAGNFLGNMANNLLVGANHEGHADSTTQSAVSAGN
                     ITIRDTQSQKQDVADLNRDAAHANQTLSPIFDREKEQQRLQQAQLIGEIGNQVADIAR
                     TEGQIAGEKAKRDPAALNQARAELEAAGKPFTEQDVAQRAYNNGMAASGFGTGGKYQQ
                     AIQAATAAVQGLAGGNLSAALAGGAAPYIAETIKQTTPDGAGRVAAHAVVNAALSLAQ
                     GKNALAGAAGAATGEVVGMIATQMYGKPVSELSETEKQTVSTLATVAAGLAGGLVGDS
                     GASAVAGAQSGKTTVENNALSELVPPRVQQDTSLAIDLGQKGQGHEEINNAIDASHIG
                     PSWGTEYKVKTNGKIEVSGGIGPALKGEMKVNNDYFSVSTGYTKEFGAKAGASIGIDF
                     GPYDFGVFGDLNRDYSSSIGYGPGSVGLSVGQDGFGFSFSIGPSIGFTGSSTVPHSEK
                     IEMSGDTTKEIYHYDFK"
     gene            complement(7217..7621)
                     /locus_tag="ACHKNP_RS19255"
                     /old_locus_tag="pgaptmp_003851"
                     /pseudo
     CDS             complement(7217..7621)
                     /locus_tag="ACHKNP_RS19255"
                     /old_locus_tag="pgaptmp_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7679..8911)
                     /locus_tag="ACHKNP_RS19260"
                     /old_locus_tag="pgaptmp_003852"
                     /pseudo
     CDS             complement(7679..8911)
                     /locus_tag="ACHKNP_RS19260"
                     /old_locus_tag="pgaptmp_003852"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
     gene            complement(8927..9454)
                     /locus_tag="ACHKNP_RS19265"
                     /old_locus_tag="pgaptmp_003853"
     CDS             complement(8927..9454)
                     /locus_tag="ACHKNP_RS19265"
                     /old_locus_tag="pgaptmp_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HGU4705938.1"
                     /translation="MRNGKYDVLSPLYSGEPVNEAEVLGAAVWLWMHSPLHRDVPLHT
                     LPDLLLPVIKHRQYVVATEQGRPVFFMSQAWLSPEAEARYLTQPAILMPQSDWNSGDR
                     MWVCDWVAPFGHTPDMSRLVRQDIFPRLCWRSLYHRGQQTGLRVMNFRGAQVSREEAR
                     QWRVQHPLAVDVPER"
     gene            complement(9464..10543)
                     /locus_tag="ACHKNP_RS19270"
                     /old_locus_tag="pgaptmp_003854"
     CDS             complement(9464..10543)
                     /locus_tag="ACHKNP_RS19270"
                     /old_locus_tag="pgaptmp_003854"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HGU4705939.1"
                     /translation="MEIVPGEQPGESDILITRKQDKYWRLGFSLDDSGTRSTGRYLGG
                     ITFSLDNPFSLSDLLYVSAIHNFPHYGGKGSKNYTAHYSVPFGYWQFSVTASDYDYHQ
                     TVAGAVEDYQYSGESQNLGMQLSRVLHRNGTQKTVLTYDVQARRSRNYINDTEIQVQR
                     RQTAAWRLGLQHRHYISQAILDAGVSWQRGTRWFGAQPAPEETFGEATALSKILQLNA
                     SFNLPFTLAGQPFSYSFRYQRQLSNTPLTPQEQFAIGNRWTVRGFDGERTLNASNGWY
                     IRNDLAWRTPLPEQALYMGMDYGEVGGAGSEYLVGRHLAGGVVGLRGQLFSTDYDVFI
                     GRPFSKPAGFMTSDVTAGFNLFRDF"
     gene            complement(10541..11116)
                     /locus_tag="ACHKNP_RS19275"
                     /old_locus_tag="pgaptmp_003855"
                     /pseudo
     CDS             complement(10541..11116)
                     /locus_tag="ACHKNP_RS19275"
                     /old_locus_tag="pgaptmp_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
     gene            complement(11220..11369)
                     /locus_tag="ACHKNP_RS19280"
                     /old_locus_tag="pgaptmp_003856"
     CDS             complement(11220..11369)
                     /locus_tag="ACHKNP_RS19280"
                     /old_locus_tag="pgaptmp_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705940.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
     gene            11791..11982
                     /locus_tag="ACHKNP_RS19285"
                     /old_locus_tag="pgaptmp_003857"
     CDS             11791..11982
                     /locus_tag="ACHKNP_RS19285"
                     /old_locus_tag="pgaptmp_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4705941.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(12035..12268)
                     /locus_tag="ACHKNP_RS19290"
                     /old_locus_tag="pgaptmp_003858"
     CDS             complement(12035..12268)
                     /locus_tag="ACHKNP_RS19290"
                     /old_locus_tag="pgaptmp_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705942.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(12364..12987)
                     /locus_tag="ACHKNP_RS19295"
                     /old_locus_tag="pgaptmp_003859"
     CDS             complement(12364..12987)
                     /locus_tag="ACHKNP_RS19295"
                     /old_locus_tag="pgaptmp_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4705943.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(13076..13585)
                     /locus_tag="ACHKNP_RS19300"
                     /old_locus_tag="pgaptmp_003860"
     CDS             complement(13076..13585)
                     /locus_tag="ACHKNP_RS19300"
                     /old_locus_tag="pgaptmp_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4705944.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT         3054 a         4150 c         3242 g         3300 t
ORIGIN      
        1 tcgaggacca ccaagcaccc gatatacaaa aataaaaaaa cacccccccc atgcaaacaa
       61 aaaaaacaac atcatcaatg atgatgcatt aagtattata tcacttgaaa tcatagtggt
      121 atatttcctt tgttgtatca ccgctcatct ctattttttc agaatgagga acagtggaag
      181 aacctgtaaa accaatactc ggcccaatag agaaggaaaa accaaaccca tcttgcccaa
      241 cggacagacc tactgaacca gggccataac caatactgct tgaataatca cgatttaagt
      301 caccaaatac accgaaatca tatgggccaa aatcaatacc aatcgaagcc ccagctttag
      361 caccaaactc ctttgtatat cctgtagaaa cagagaaata atcattgtta actttcatct
      421 cacctttaag ggcaggacct attccacccg aaacctcaat ctttccatta gtcttgactt
      481 tatactctgt cccccatgaa ggaccaatat gagaagcatc tatcgcattg ttaatttctt
      541 cgtggccttg tcctttttgt cctagatcta ttgccagaga agtatcctgc tgtacgcgag
      601 gcggcacaag ctcagacagc gcattattct caacagtcgt cttacccgac tgtgcgccgg
      661 ctaccgctga agcccccgag tctcccacca gaccaccggc caggccggca gcaacagttg
      721 ccagcgtgga gaccgtctgt ttctctgtct cactcagttc actcaccggc ttaccgtaca
      781 tctgtgtggc tatcattccc accacctcgc cggtggcagc gcctgcggca ccagcaagag
      841 cattcttacc ctgtgccaga gacagggctg cattcactac tgcatgagct gccacacgtc
      901 ccgcaccatc cggggtggtc tgtttgatag tttcagcaat atacggcgct gcaccacctg
      961 ccagggctgc actcagatta ccgccggcca gtccctgtac cgctgccgtt gcggcctgta
     1021 tcgcctgctg gtatttgcct cccgttccaa aacctgaggc cgccataccg ttattgtagg
     1081 cccgctgtgc cacatcctgt tccgtaaacg gctttcctgc tgcctcaagc tctgcgcggg
     1141 cctgattcag tgctgccggg tcccgcttcg ctttctcacc ggctatctgt ccttccgttc
     1201 tggcaatatc tgccacctgg ttaccaatct caccaatgag ttgcgcctgc tgcagtcgct
     1261 gctgctcttt ttccctgtca aagatggggg acagcgtctg gtttgcatgt gctgcgtcac
     1321 ggttcaggtc tgccacatcc tgtttctggc tctgcgtatc ccggatggtg atattcccgg
     1381 cagacactgc cgactgcgtg gtactgtccg catggccttc atgatttgca ccgaccagca
     1441 gattgtttgc catgttcccc aggaaatttc cggcaacact gccaccagta ctgagccctg
     1501 cactctgatg ttccgtttta aagtcggccc ggttttcaat gtcactgaag cccagtgtgc
     1561 cggtatccag cctgttttta tctgccgttg ccgtggacgc aatcaccgca ccgttcagtt
     1621 gcgtatgctg tccggtggtg acatcaaacc cgcccctgcc ggcaaaaatc cccgtctgct
     1681 cctgtacact gtcgtagtta ctgtgcattt tatcccggct gaggttcaca ctcgccgaac
     1741 cactcatgga cccgaaagtg aagctgcctc cggcgctggc attctgctgc ttactgtcat
     1801 aacggtcact gtcctgctca ctggtcagtg tcagatgacg accggcatcc actttcaccg
     1861 tttcaccgcc agcctgcgcg ccggtaagcg tggtgtcccg cccgctgata atggtcagcc
     1921 ggtttcccgc atccaccatg gtttctgtat gggtggtacc gttgcctttt tcactgccct
     1981 taccctggtt tgcgctggct gaaacactga taccccagcc tccggagccc acaccaacac
     2041 cgacacccac gctgccacca ctggatgtgt tctttccttc cagctttgac gtgttttctg
     2101 ctgataccag attcacgtca cggtttgcct gcagcagaac gttgttcccg gcctttatct
     2161 gactgccctg tatcgtcagg tcaccatcca cgcccttcac gccggagccg gtggcctgaa
     2221 tactgaggtg attacctgcg gtcagtgtac tgcccttcgc caccgtctgc tctgactgct
     2281 gttctgattt cgaagactgg ctgccgtaag agaggctgat gcccaccgtg ttattatttt
     2341 cggcatcggc tccctgcgcg tctgccagtc gtccggcctg cacggcctga gcccccgaga
     2401 gcgcggcctt caccccctgc agggcttcaa gccggctgtt tccggcagat ttcgccgcct
     2461 gtacggtctc caccgtcgta ttcagggcgc tgcccgctgt gccggacagg gcaagcgtca
     2521 gaccggaggt tttctgctcc accgtatgtg tctgtacatg ctggtctgtg gcggcggtaa
     2581 tgctgacctc cttaccgctg agtgccatgt ccctgccggc aatcaggtca gagccgttga
     2641 cggtcaaccg gttacctgcc tgaagggaaa catcaccgtg aacactcccc accgtgctgc
     2701 ctttctgtgt ggtgtctgtt gcggtgtccg tgactttcag actctggctg cccacgctga
     2761 atcccacacc tccggagctc atcagcccgg attttttctc ctgtttcaga tgtgtttcac
     2821 ggttgctttc tgccagcgta ccgatattca ggttatttcc ggcagccagg gagacacggt
     2881 tatccccggc cacactgctg cccgtgacgt tcagattatg tccggcgccg atactgaccg
     2941 tatccccatt cagttcactg cccgtcatgg tccggcggtc agtgacatca tgcgtggttg
     3001 tggtggtttt cgataacatc ccgctgctgc cggtcacttt atggtattca tccagcgagc
     3061 tggtattctg tgcaccggtc agcgtcacgt catgacccgc catcagggcc aggcctttct
     3121 gagctgacag cgatgccgcc tgtgacgtca tatcccgtcc ggcggtcagc gtgacatcac
     3181 cgttaccggc aagcgtgctg cccgtactct gtgtgacgcc ctgcgacaga cgattatttt
     3241 tgtcccaggt aatattgtcc tgtgcggatg tggtcactgt ggaaagcgtc atatccccgc
     3301 ccgcactgag ttgcgtcagg ctgtccttac cctgattcac caccgttgca gccgtcaggt
     3361 tcatatcccg tccggcctgc agcaccagtc tgccctggtc gttctgcaca tacacgccgg
     3421 ccacactgtc gatactggta cgttcaaaat ggttctgtcc ggcgtcactc tgtgccgtac
     3481 gggtggtggt ggtcagactg atatcccgtc cggccatcgc cagcaggctg tccgtggcct
     3541 gcagcagccc gccggtactg ttaatatccg tccatgccgt caggctgaca tttgctccct
     3601 gaatggtgcc ggcctggttg tgaatgttct ctgcactgag ctgcacggtc tgtttccctg
     3661 ccagtttacc gctgttaaac aggtcaccat ccagcttcat caccatgtcc ctgccggcaa
     3721 tcagcgcacc ggcactgttc acatcaccgg gttttacacg ggcatacacc tgaggtacct
     3781 gaaccgtctg cgtgctgccg tccggcagtg tcaccgtggt gttaaccagc cagacaatat
     3841 caccggtcag taacgccatc tgctcagcag tcagcgccac acctggtgtc aggttgtatt
     3901 gcttaccaaa tgcaataccc gcatccatta acgccttaaa ctgctcttca tcattactgt
     3961 atccgtccag gtaccgttgt ccggtcagag cgacaacctg ctcacgaatc agacgctgtt
     4021 cgtaaaaacc atcccccagg cgtttgagca tgttatcccc gttctgagaa aacgccttct
     4081 gcatgtaatc actgcccagc cacgtcttct ggttcgtaaa acgcgggtcc gtttcaacca
     4141 gatatggcac attcactgcc ggatttgttt taaacagact gctgtccggc agacgggtat
     4201 ccggcccggc aacatggata ctgccctgag gtgtactgac ttcgaactgc tgccccggca
     4261 acagaatgac cggttcaccg gcagacacca ccgggcgaag cggtatccgg tcagtagcgg
     4321 ctactgccgc atccacattt ccggcctgtc ctgtctgtac atccgttccc tgtggcttca
     4381 ggggagaaag cgtgacctgg ctgccctgaa tctgtccgtg gctggtgagc tctcccggct
     4441 tcagcgtaat gctctgaatt acagtgggcg gagtgtactg agaggttttc tccccctgct
     4501 tatcctttcc ttttttcctg atacggtaat aatacgtgga tgttcctgag tccgtaacat
     4561 gccgctcgcc actgacactg acattttcca cgcttcctga cggaatggtc agcaggccgc
     4621 ctgcaacaac ctgactctta tcattaagca gtttgtctgc cacaatcgtc atgccggcac
     4681 cggacagaat ttttcccggg tcggagtctt ttacccgggt ttcttccacg gtacgggtgt
     4741 aatcgtactg cgtgaattta tctctggcac cttccggtgt atgcagtttt ttcagtgagt
     4801 tactggaatc gttatccaca taaacgcccg gttcaccggc gctccagcgt ttatctgagc
     4861 cgctgagctg atactcagtc accttttccg tggaaaccac cacgcgctcc gtggtgaaat
     4921 ggtcattgac gttattgatt tgtccggcac tcagtgccag atatccggcg gcttcaattg
     4981 tggcactgtg attattcagt acaccggctt tccccgtggc ggcgccattc gcatccagcg
     5041 tgccaccgat atgcatatcg cctgcactgt aaatcaggct gtgcgtccgg ttattcaggg
     5101 tctgtacccc taagtccaca cgctcacgcc cggccagcgt cgccgccacg ccattttcat
     5161 ccagattatt caatgtggca gcgctgacac caaccgcatc gccgtagatt cgcccggtac
     5221 cggaattcgt cagggtgttc acctgaagac gcgtatattt cccgtcaatc agcccacggt
     5281 tcagcagtgt gtccgtcacg ttaagccagg tctgcccggc actgatttca cctttctctg
     5341 tgttttcaag gcggatgctg ttcaggtcaa gccggctgcc tgccagcaga ctgccggtat
     5401 tagagacatc gccattaacg gaaagcgtaa ggtttccgtt cgcaatagtc tgaccactgt
     5461 tgctgaacgt tgtgttactt ttcagcgtta tatcgcccgg ggaaagcagt ttcccgtcac
     5521 caccgagttg accagcatcc acagagactg actgcccggc cttaaccaca ccgtcacggt
     5581 tcgaaaacgc cagctgacga ccactgacag acaatgtcgc gccggaagag agcacgccgt
     5641 cattattact gagcgtcctg tctgccataa ccgtaagcgc cctgtctgcc agaatactgc
     5701 cctcctggtt atcaaggtcc tgcgtcgtga tgtgtattgt ctgcgcttcc agcccctggc
     5761 cggtattctg cgtgtggcga ttgataaact gcagggcgtt cagcgtaatg gcagaaccac
     5821 tgcgaatcag accagagacg ttgtccacaa cgccccgtac actgtcgaac agaatgtcgc
     5881 ccacagactg tatctgtccg tggctgttct gcaggcctcc cgtatgcaga cggagttcac
     5941 cttcactgac cagccggcca ccctcacggt tatccagcga ggtggtgctc aggtcagctg
     6001 tcgttttcgc ccccacggtc ccgccccggt tatccattgc tccggtactg acggtcagtt
     6061 cctgcaggct gttcagcgta ccgcctctgt tatccagcgt ctggcctgcg gtattaatgc
     6121 tgatgccttt cccctgaata agaccggact gattgttcag cgcctggctg ttccagccga
     6181 gcaggccgtt tgccaccacc tgaccggatg tattgttaac cacgcctgca gtcagggaca
     6241 gagcatctcc cgacaaaagc acaccggcag tgctgtccag tgtgccggca ttcagcgtca
     6301 tcgccccctg actgataacc ccgccccggt caccactgtt acggttactc agtgtgtcag
     6361 cacggagatt cagcgatgca ccgctctgta tcaggccgct ggcatcgttg atgatatccg
     6421 tcgaggaaag ggtcagtgac tgctgtgcca ccagtctgcc gccctgatta tcaaaccgtg
     6481 tgacatccag cctgctgtta ccgttgctga ccaccgtccc ctgctgattt tccagcacat
     6541 tcaggtgagt attcaggtcc ccgccggcgg caagcgtccc ttcctgatta ctgacatcgc
     6601 caccactcag caacatatcc gtcccggcct tcagtgcacc tttctgattc atcagcgtac
     6661 ctgacgatat ctgtaatgac gcctgactcc ccaacacgcc ctgacggtta tcaagcgttc
     6721 cggcgttcag ggtcatatcc ttctgaccca ccataaatcc ctgctggtta ttcagcatac
     6781 cagcccgcac tgtcagggta ccggcgctgc tgataccacc ccgtgttttc gtgttccggt
     6841 tactaagtac cccgtcctgt acatccagcg tcatgtcact gccggactga atcagaccat
     6901 catcagagac cagttcgctg cctgacacag acagcgtttt ctgtgaaagc agtctgccgg
     6961 acgtattatc cagcgcacca tccagcgtca cggacagccc gtcagacacc accgtaccct
     7021 ggtggttatt cagactccct gccgaaatat ccagcctgcc ggcggactcc attcgtccct
     7081 ccgtattgct gacagactgc tgtgttttta tcctgcccgt tccggcagca gctgaaatca
     7141 gtccgttgtc gttattcagc gcctgtgtat taatcaggat gtcactgctg gcaagcagac
     7201 gtccctgctg gttatccacg gcaccggttg tcttcacctg cagactgcct gactgcgccg
     7261 cctgcacttt ccccttgcga ttatcaaggc tcagtgcatc cagacgcacc gcgccatttg
     7321 ccgcaagcag cccttcccgg ttatccacgc ttcccggcag actcagcgaa aaatcccctg
     7381 ttcccgtctg aatcagactg cctttacgat ttgacaggct ttgtgccgat atctgcagtg
     7441 tgtcggcatt tatcgtaccg ccattattgc tgaactgccc ggcagtccgg gcggaaagcg
     7501 taccggcact cagttgcgca ttctgtgtag acagatttcc actggttgca ttcagctcag
     7561 tatgttgtcc ctgtatccgg ctgttgccta aatccagccc ctgccccttc agttgcatgt
     7621 ccccaccgga cagaacctgt ccgtgtgccg ccagttcgcc ggtggtactg acagtcaggt
     7681 tgcccgctgc cgccagacgt ccgtcatctt tcaccccggc ggccagcaga ctccgctcac
     7741 cgccgatcag acgcgctgtc tgaatccggg tgttgcgacg tgatgccaca gagcctgtat
     7801 gggtgaatac accatcactc tgtatctgcg tatcctgccc cgcataaaca gaaccactgt
     7861 tatgcagctc ccgggttgtc gccagatgca catccgtttt cgcactgata ctgccactgt
     7921 tttcaattcg tccgtcagcc gtcagggtaa ccgcccctgc ctgtgcacca atatgaccgg
     7981 cgttacgaac acccaccccc gtttccgtac cgacaaggcg aatttttccg gcatacattc
     8041 cgccaagact ggccacatcc agtgccatct gaggctgtga agagacatct ccggtctttt
     8101 tctcaatctg cccgtgcgcg atatccacat tgttacgtcc tgtggtcact ttcaggtcct
     8161 gcgcccagat acccgcatta atcttcacag accgggcaat gatatcggta taatccgtgc
     8221 gcgaggcatc catccctttg cccgtaatct gaatctcacc acgttcaaca ctgaatccgg
     8281 tcagactgcc gtttttcatc tgcggctgcc ccgtggtcag cgtggcccgg ttcgcattaa
     8341 taaaaccgca cccgtcgcaa ctgatgccgg ccgggttcgc aatcaccacc tgcgcctttt
     8401 tcccggcaac ctcaacaaaa ccgttcaact ggctgggatt gcgactgttc acttcattaa
     8461 gaatgattcg ggcctctccc ttcgccagcc acgggttccc ggccaccatg ccacccagct
     8521 gagtctgcac attgttatgg gcattattca gaatgacacc cttgttaccg acgtcgaact
     8581 ggctgtatgt gttatgtgaa accccaccgg cagaaggcgt ctgaatattc acctgcggaa
     8641 tgccgctggc cgtctgcata atcgtgggct gctgatgccc cggtgcgtca tggtctgcga
     8701 caatgcctgc agcactgaca gaatgcacca tccccgacgc cagccacaaa gagaatgcaa
     8761 gcggcgtgac acggcagata cgttgcctgt gtggatatcc tgtgcgggag gacagtgatg
     8821 taccggcgcg accggaacgg gcaatatctg ccaccaccat cagcattccc cgggctttgt
     8881 taaaaataat gcggtaacaa tttctgttca ttctgataaa cctgacttat ctctccggca
     8941 catccacagc aagcgggtgc tgcacacgcc actggcgcgc ctcttcacga ctgacctgtg
     9001 caccccggaa attcatcacc cgcaacccgg tctgctgccc acggtggtac agcgaacgcc
     9061 agcacagacg gggaaaaata tcctgccgga caagacgact catatccgga gtgtgtccaa
     9121 aaggggcaac ccagtcacag acccacatcc ggtcgccgct gttccagtcc gactgaggca
     9181 tgagaattgc cggctgcgta agataacgcg cctctgcctc cgggctgagc caggcctggc
     9241 tcatgaagaa aaccggtcgc ccctgctctg ttgccaccac atactgtcgg tgcttaatca
     9301 ccggcagcag caggtccggc agggtatgca gtggcacatc acgatgcagg ggggagtgca
     9361 tccacagcca gacggccgcc cccaggactt ccgcctcatt gaccggctca ccggaataca
     9421 ggggactcag aacgtcatat ttcccgttac gcatgaaact ttctcagaaa tcccggaaca
     9481 gattaaaacc ggctgtcaca tcactcgtca taaaacccgc cggctttgaa aacggacgac
     9541 cgatgaaaac gtcataatcc gtactgaaca gttgccccct cagccccaca acaccaccgg
     9601 ccagatgacg tcccaccaga tattcactgc cggctccccc cacctcgccg taatccattc
     9661 ccatatataa cgcctgctcc ggcagtggcg tacgccaggc caggtcgttg cggatatacc
     9721 agccgttact ggcattcagc gttcgctccc cgtcaaaacc acgaaccgtc cagcggttac
     9781 cgatggcaaa ctgttcctgt ggagtcagcg gggtattgct cagctggcgc tgataccgga
     9841 aggaataact gaagggctgc cctgcaaggg taaagggcag attaaaggat gcattcaact
     9901 gcaggatctt actgagcgcc gttgcctcac cgaacgtttc ttccggcgcc ggctgtgcgc
     9961 caaaccagcg ggttccccgt tgccagctga cgccggcatc cagtatggcc tgcgaaatgt
    10021 aatggcggtg ctgcagtcca agtcgccagg ccgccgtctg ccggcgctgg acctggattt
    10081 ccgtatcatt aatgtagtta cgcgacctgc gggcctgaac atcgtaggtc agcaccgttt
    10141 tctgcgtccc gttacgatgc agcacccggc tcagctgcat acccagattc tgactctcgc
    10201 cactgtactg gtaatcctca accgcaccag ccaccgtctg atggtaatcg taatcactgg
    10261 cggtgacgga aaactgccag tagccgaaag gcacagagta atgggccgtg tagtttttac
    10321 tgcccttacc cccgtaatgt ggaaaattat ggatggcaga aacatacagc aggtcactca
    10381 gggaaaaagg attatccaga gaaaatgtta taccaccgag ataacgccct gtgctgcggg
    10441 tgcctgaatc atcaagcgaa aaccccagtc gccagtattt gtcctgtttg cgggtaataa
    10501 gaatgtcact ttcccctggc tgctcaccgg gcacaatttc catatcagcc tgcacacccg
    10561 gcagtcgttg catattctcc agcccctgct caatgtcccg taaatccagc agagagcctt
    10621 cgtgtgccgg gaatgaggaa tacaactgaa tatagtcacc actgtccggt gtcagacgca
    10681 catatcgcac aacaccggga ataataacca gccggaggat accgcttttt aaatcctgcg
    10741 caggtgccag aacccgggtg gtgacataac catgatcgac cagacggttc tgcaatgtac
    10801 tcatcagcag attaattcct ttcgccccca ggcaatgccc gaccgccccg ttggcgattt
    10861 tttgtaaagg caaccagtgt ggtaacgcat cagccccctc cagttccgtc tgtttaatct
    10921 gaaaacaggg cgtttcaaca ggaaaattaa tcttacgggc aaaagagcca ggtgcagaaa
    10981 ggcgaacatc aggggctgaa ggcgtcagtt gttgctcaag ggcttcctga cgctgctgct
    11041 gaatgataaa tgtatcctct ccactacgcc ggatatccgc ggcctgaaga gataaaggcg
    11101 ataataaaac agacatcagt gtcagtgaca gcaaggacgg acagcgccgg aatacgcttt
    11161 gttcctcagc cctctcgttc ctgcaacata attttccagt caaacgaaaa accttttatt
    11221 catgtcatac caacaatcac cagcctcctg acgatacctc ccaccatgtt ttgcgatggc
    11281 acgctaccgc ccttccggta tttacccgca tcaaccggta ccagaatgct gagtaacaaa
    11341 ccagctagaa ttgacttaat tttgatcatt atagaataat gatctgaact acagcaacat
    11401 gacagattta tcgatcgata taacacaaat gaacagttct ttattctgac aaatatcgca
    11461 aaaacagcac attacaaaat ttataaccat aattactgga aaagtgcaga aaattattcc
    11521 actgaatgct tttaatacag ttggttagct taaaaaatag caatttttga tttcagtcat
    11581 tgaaagttgc aatgtttgac gattcaacag catttaagtg agtttcagga tatgggaagg
    11641 acatgaaagg aggagcgcaa taaaatacac aggaagaagg ggaaaatgtt tcgtaaaaac
    11701 agaaaattaa cagcgcacat caggaaacgc agtctcctga ttcagtgcta ccacatgaaa
    11761 atttagttaa ctgttaccgt actaaaattt atgtcagaag atgttccatt accgaaagtc
    11821 aatcagcgat acaaagacga tcatggtgcg cttgtcaccc tgaccagtgt tgaggaaacc
    11881 cgggtggtgt ttatgcagga cggctatccg catccctgca tgcgaccgat gtacaacttt
    11941 ctgggaaaat tcaaacccga accccgggaa gaaacagaat aaaacgaaat ccctgccggc
    12001 aatcacacta cctgatagca gcataatcca cttattaatt ctcatccccg gagacaagcc
    12061 cagaaaacca ctccgggtgt tcactgtatg ccgtgttcag cgcatccctg gaaacgttta
    12121 tataaaagct gtcctgcaat ttgcgggtaa caacttctgc caggcgattc cggttatcca
    12181 ccagtccggc ataacctctc tctgccagcc actcattcag ttcatgttcc agagacagat
    12241 tgataatatc ataatcggac cttgccattc ccgtttcctt agcacatgtt ttcaggcacg
    12301 gggataaagt ataaagaatg tcatactgct gctgccagag caatattccc cctgatggtt
    12361 gtatcagaga tggtatgccg gtactttgcg ggttgttccc gtcaggttat cgtgcacctc
    12421 cacggtcgtg gtcaccaccg gcattccggc ctttctcagc ctcagaacat cagccgcaat
    12481 acgctgactg ccaaaccaga acaggccgtc cagtgcagtc accagtaaac ccgcctccag
    12541 tgcatacttc agccgttcac ggggcgcttt cacttcccgg gcaacctgct gatatggcaa
    12601 tgatgtgttt tcattcccaa ccacgcgacg aatacggtga gacagatgat accggtatgt
    12661 atccggcaca ccagaaaggc tggccttcag gctgtacacg cagccaaacc gtttatcgtt
    12721 aaacaccaca tttttctggc tgatgcccca ttcttcacgc agcgcggcaa tcaattgcgg
    12781 tgtacgggta agcagcaagc gaaaaggcag ttcaaaactg gtgacgtaat ccacattcag
    12841 cagtgcaata cgcaaccgtt cttctggccc ggcctctgtc tgccggcact cctccagaac
    12901 atcctgccac tgccggcgaa gacgggggga ttcgttccgt tctgtaaaac agtatttatc
    12961 cgccagatag tcaattcgtg tacgcatact gaaaggaatt ccgtataaag attcagcaga
    13021 caaaacttta tcagccagta aaaattaacg gaagcattga tattcctccc gacagtcacc
    13081 ggatgattgt tgcaataacc tgcgacataa aggactgaac agccgttttt aacgccacgt
    13141 attgccctga tgaaccaggc cgggccaccc gaaaacatct ttccacccgg cattgtccgc
    13201 cagcacttat caccgaccag gtctgttgca gtaaatccac cagttgcgca cattcttcat
    13261 cagccatgac gccgactcgc ggatggcaaa tactcgtctg gctgagcagc aacagacatc
    13321 gtatctgtgc tccttcatgt tgttcccgca accagcgtaa taccaccggc gctgatgggc
    13381 tgaccgtgtg attatgctcc gtaatacgtt cgtgtacccg tcggtgaaag gaactacaaa
    13441 atgtctgaat ctgttgacca tagattcctt ctgccgaata gagcgtgaag tgaaatgcca
    13501 acagatttgg ccagtggtcc accgcctgct gaacaaaacg ccggatttcc cccggctctg
    13561 aaattaaggc ttcggttatt tgcacgaaat gttccttttc aacccctgtt gaattttgtc
    13621 aagccggtgc agacacagta aagcgcgcat caacacctga aaggtacgat acaaacacaa
    13681 gttacaaaca ataagaacgg aaggacaatt aatcagtgag gaataataac taaattaacc
    13741 aaaaga
//
LOCUS       DAZFWL020000125        13634 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFWL020000125 DAZFWL020000000
VERSION     DAZFWL020000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.125"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            52..909
                     /gene="focA"
                     /locus_tag="ACHKNP_RS19305"
                     /old_locus_tag="pgaptmp_003861"
     CDS             52..909
                     /gene="focA"
                     /locus_tag="ACHKNP_RS19305"
                     /old_locus_tag="pgaptmp_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HGU4705945.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            964..3246
                     /gene="pflB"
                     /locus_tag="ACHKNP_RS19310"
                     /old_locus_tag="pgaptmp_003862"
     CDS             964..3246
                     /gene="pflB"
                     /locus_tag="ACHKNP_RS19310"
                     /old_locus_tag="pgaptmp_003862"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU4705946.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3438..4178
                     /gene="pflA"
                     /locus_tag="ACHKNP_RS19315"
                     /old_locus_tag="pgaptmp_003863"
     CDS             3438..4178
                     /gene="pflA"
                     /locus_tag="ACHKNP_RS19315"
                     /old_locus_tag="pgaptmp_003863"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HGU4705947.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4260..4850)
                     /gene="ycaK"
                     /locus_tag="ACHKNP_RS19320"
                     /old_locus_tag="pgaptmp_003864"
     CDS             complement(4260..4850)
                     /gene="ycaK"
                     /locus_tag="ACHKNP_RS19320"
                     /old_locus_tag="pgaptmp_003864"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HGU4705948.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            4950..5858
                     /gene="ycaN"
                     /locus_tag="ACHKNP_RS19325"
                     /old_locus_tag="pgaptmp_003865"
     CDS             4950..5858
                     /gene="ycaN"
                     /locus_tag="ACHKNP_RS19325"
                     /old_locus_tag="pgaptmp_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4705949.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(5859..7289)
                     /gene="ycaM"
                     /locus_tag="ACHKNP_RS19330"
                     /old_locus_tag="pgaptmp_003866"
     CDS             complement(5859..7289)
                     /gene="ycaM"
                     /locus_tag="ACHKNP_RS19330"
                     /old_locus_tag="pgaptmp_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU4705950.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPFLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTASAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(7498..8646)
                     /gene="ycaD"
                     /locus_tag="ACHKNP_RS19335"
                     /old_locus_tag="pgaptmp_003867"
     CDS             complement(7498..8646)
                     /gene="ycaD"
                     /locus_tag="ACHKNP_RS19335"
                     /old_locus_tag="pgaptmp_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4705951.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            8961..9587
                     /gene="ycaC"
                     /locus_tag="ACHKNP_RS19340"
                     /old_locus_tag="pgaptmp_003868"
     CDS             8961..9587
                     /gene="ycaC"
                     /locus_tag="ACHKNP_RS19340"
                     /old_locus_tag="pgaptmp_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HGU4705952.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(9623..10486)
                     /gene="dmsC"
                     /locus_tag="ACHKNP_RS19345"
                     /old_locus_tag="pgaptmp_003869"
     CDS             complement(9623..10486)
                     /gene="dmsC"
                     /locus_tag="ACHKNP_RS19345"
                     /old_locus_tag="pgaptmp_003869"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HGU4705953.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(10488..11105)
                     /gene="dmsB"
                     /locus_tag="ACHKNP_RS19350"
                     /old_locus_tag="pgaptmp_003870"
     CDS             complement(10488..11105)
                     /gene="dmsB"
                     /locus_tag="ACHKNP_RS19350"
                     /old_locus_tag="pgaptmp_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HGU4705954.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(11116..13560)
                     /gene="dmsA"
                     /locus_tag="ACHKNP_RS19355"
                     /old_locus_tag="pgaptmp_003871"
     CDS             complement(11116..13560)
                     /gene="dmsA"
                     /locus_tag="ACHKNP_RS19355"
                     /old_locus_tag="pgaptmp_003871"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HGU4705955.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
BASE COUNT         3588 a         3530 c         3204 g         3312 t
ORIGIN      
        1 tataatgctt tgttagtatc tcgtcgccga cttaataaag agagagttag tgtgaaagct
       61 gacaaccctt ttgatctttt acttcctgct gcaatggcca aagtggccga agaggcgggt
      121 gtctataaag caacgaaaca tccgcttaag actttctatc tggcgattac cgccggtgtt
      181 ttcatctcaa tcgcattcgt cttctatatc acagcaacca ctggcacagg cacaatgccc
      241 ttcggcatgg caaaactggt tggcggcatt tgcttctctc tggggctgat tctttgtgtt
      301 gtctgcggag ccgatctctt tacttccaca gtgttgattg ttgttgctaa ggcgagtggg
      361 cgcatcacct ggggtcagtt ggcgaaaaac tggctaaatg tctattttgg caacctggtc
      421 ggcgcactgc tgtttgtact tttaatgtgg ctttccggcg agtatatgac cgcaaatggt
      481 caatggggac taaacgtcct acaaaccgcc gaccacaaag tgcaccatac ttttattgag
      541 gccgtctgcc ttggtatcct ggcaaacctg atggtatgtc tggcagtatg gatgagttat
      601 tctggccgta gcctgatgga caaagcgttc attatggtgc tgccggtcgc gatgtttgtt
      661 gccagcggtt ttgagcacag tatcgcaaac atgtttatga tcccgatggg tattgtaatc
      721 cgcgacttcg catctccgga attctggacc gctgtcggtt ctgcaccgga aaatttttct
      781 cacctgaccg tgatgaactt catcactgat aacctgattc cggttacgat cggtaatatt
      841 atcggcggtg gtttgttggt tgggttgaca tactgggtca tttacctgcg tgaaaacgat
      901 caccattaat ggttgtcgaa gtacgcagta aataaaaaat ccacttaaga aggtaggtgt
      961 tacatgtccg agcttaatga aaagttagcc acagcctggg aaggttttac caaaggtgac
     1021 tggcagaatg aagtaaacgt ccgtgacttc attcagaaaa actacactcc gtacgagggt
     1081 gacgagtcct tcctggctgg cgctactgaa gcgaccacca ccctgtggga caaagtaatg
     1141 gaaggcgtta aactggaaaa ccgcactcac gcgccagttg actttgacac cgctgttgct
     1201 tccaccatca cctctcacga cgctggctac atcaacaagc agcttgagaa aatcgttggt
     1261 ctgcagactg aagctccgct gaaacgtgct cttatcccgt tcggtggtat caaaatgatc
     1321 gaaggttcct gcaaagcgta caaccgcgaa ctggatccga tgatcaaaaa aatcttcact
     1381 gaataccgta aaactcacaa ccagggcgtg ttcgacgttt acactccgga catcctgcgt
     1441 tgccgtaaat ctggtgttct gaccggtctg ccagatgcat atggccgtgg ccgtatcatc
     1501 ggtgactacc gtcgcgttgc gctgtacggt atcgactacc tgatgaaaga caaactggca
     1561 cagttcactt ctctgcaggc tgatctggaa aacggcgtaa acctggaaca gactatccgt
     1621 ctgcgcgaag aaatcgctga acagcaccgc gctctgggtc agatgaaaga aatggctgcg
     1681 aaatacggct acgacatctc tggtccggct accaacgctc aggaagctat ccagtggact
     1741 tacttcggct acctggctgc tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt
     1801 acctccacct tcctggatgt gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa
     1861 caagaagcgc aggaaatggt tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg
     1921 cgtactccgg aatacgatga actgttctct ggcgacccga tctgggcaac cgaatctatc
     1981 ggtggtatgg gcctcgacgg tcgtaccctg gttaccaaaa acagcttccg tttcctgaac
     2041 accctgtaca ccatgggtcc gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa
     2101 ctgccgctga acttcaagaa attcgccgct aaagtgtcca tcgacacctc ttctctgcag
     2161 tatgagaacg atgacctgat gcgtccggac ttcaacaacg atgactacgc tattgcttgc
     2221 tgcgtaagcc cgatgatcgt tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg
     2281 gcgaaaacca tgctgtacgc aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt
     2341 ggtccgaagt ctgaaccgat caaaggcgat gtcctgaact atgatgaagt gatggagcgc
     2401 atggatcact tcatggactg gctggctaaa cagtacatca ctgcactgaa catcatccac
     2461 tacatgcacg acaagtacag ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt
     2521 atccgcacca tggcgtgtgg tatcgctggt ctgtccgttg ctgctgactc cctgtctgca
     2581 atcaaatatg cgaaagttaa accgattcgt gacgaagacg gtctggctat cgacttcgaa
     2641 atcgaaggcg aatacccgca gtttggtaac aatgatccgc gtgtagatga cctggctgtt
     2701 gacctggtag aacgtttcat gaagaaaatt cagaaactgc acacctaccg tgacgctatc
     2761 ccgactcagt ctgttctgac catcacttct aacgttgtgt atggtaagaa aactggtaac
     2821 accccagacg gtcgtcgtgc tggcgcgccg ttcggaccgg gtgctaaccc gatgcacggt
     2881 cgtgaccaga aaggtgctgt agcgtctctg acttccgttg ctaaactgcc gtttgcttac
     2941 gctaaagatg gtatctccta caccttctct atcgttccga acgcactggg taaagacgac
     3001 gaagttcgta agaccaacct ggctggtctg atggatggtt acttccacca cgaagcatcc
     3061 atcgaaggtg gtcagcacct gaacgttaac gtgatgaacc gtgaaatgct gctcgacgcg
     3121 atggaaaacc cggaaaaata tccgcagctg accatccgtg tatctggcta cgcagtacgt
     3181 ttcaactcgc tgactaaaga acagcagcag gacgttatta ctcgtacctt cactcaatct
     3241 atgtaattag atttgactga aatcgtacag taaaaagcat acaataaagg ctccacgaaa
     3301 gtggggcctt ttttagcacg agagcctttt ttgtcagcta tctatacttt aaggtgactg
     3361 ccaaaacaga ctcgacgtag ccttcgagct gcgcaccaac acggcctcag atgggccaca
     3421 tctggagaaa caccgcaatg tcagttattg gtcgcattca ctcctttgaa tcctgtggaa
     3481 ccgtagacgg cccaggtatt cgctttatca cctttttcca gggctgcctg atgcgctgcc
     3541 tgtattgtca taaccgcgac acctgggata cgcatggcgg taaagaagtt accgttgaag
     3601 atttaatgaa agaggtggtg acctatcgcc actttatgaa cgcttccggc ggcggcgtta
     3661 ccgcatccgg cggtgaggca atcctacaag ctgagtttgt tcgtgactgg ttccgcgcct
     3721 gcaaaaaaga aggcattcat acctgtctgg acaccaacgg ttttgttcgt cgttacgatc
     3781 cggtgattga tgaactgctg gaagtaaccg acctggtaat gctcgatctc aaacagatga
     3841 acgacgagat ccaccaaaat ctggttggag tttccaacca ccgcacgctg gagttcgcca
     3901 aatatctggc gaacaaaaat gtgaaggtgt ggatccgcta cgttgttgtc ccaggctggt
     3961 ctgacgatga cgattcagcg catcgcctcg gtgaatttac ccgtgatatg ggcaacgttg
     4021 agaaaatcga actcctcccc taccacgagc tgggcaaaca caaatgggtg gcaatgggtg
     4081 aagagtacaa actcgacggt gttaaaccac ctaagaaaga gaccatggaa cgcgtgaaag
     4141 gcattcttga gcagtacggt cataaggtaa tgttctaatt tccccgtaaa gcggcaactt
     4201 tattgagttg ccgctttttt attccgcgct tcattctgct acagcgtttt taacacattt
     4261 tatagtgcat ctaccatatc ccgtacctga gataataacg actgataata gctcgcatga
     4321 agttcttgcc catcgaatac cactgtatta cacaagaaag tgacatcggc atcttcaata
     4381 ccaagataac tgcacatatt tttcaaataa tcgcttatat ttttttccca gcccatctgg
     4441 acaaatgatt ctttgtctcc tccaaccagc gccacccaac gaactttatt gaatggtaat
     4501 ttgtgcccat ctccataagc cagcccatta ttccatactc tgtcaatata tccttttagc
     4561 attgccggga agctatacca ccagagagga aaaaccacca ctaacgtgtc atgttcaagc
     4621 agctctgaat aaagctgatg aacctctgga gaataacgtt tatccatatt cttccagtct
     4681 ggttcatctt ccggcgtcat cactgggttg aaattacgcc gatataaatc gagttccgtc
     4741 acctgtattt tccgctccat tgcccgctga tggatcgcat cagcaatatt tgcggtcaat
     4801 gaatcatgac gaggatgggc ccataccaaa taaatacgtt cagactgcat aattttacct
     4861 ccacgttaag ttctggagcg cactttacgc aggcggcata atcctgtaaa cgtgatgatt
     4921 caggatgagt taatgaagga ttttcactaa tgcggatgaa tatgtctgac tttgccactt
     4981 tctttgccgt cgcccgtaat caaagctttc gtgcagcggg cgatgagtta ggcttatcct
     5041 cgtccgccat tagccatagt attaaaacac tggaacaacg tcttaaaatt cgcttattca
     5101 atcggacaac ccgaagcgta tcgttaacgg aagcgggttc taatctctat gaacgattgc
     5161 gcccagcttt cgacgaaatt cagatcatgt tagatgagat gaatgatttt cgtctgacac
     5221 cgacaggaac gctcaaaatc aatgccgccc gggtagctgc acgtatattt ttaatgcctt
     5281 tactggttgg tttcacacgt gaatatcctg atattaaagt cgagctcaca actgacgatt
     5341 ctcttgtcga tatcgtccaa cagggctttg acgctggcgt tcgacttagt ggcatcgtcg
     5401 aaaaagatat gatcagcgtg gcgatcggcc caccagtgaa attatgtgta gcagcaacgc
     5461 cagagtactt tgcaagatat ggcaaacccc gccatcctca tgatctgctt aatcatcaat
     5521 gtgttgtttt tcgttatccc agcggcaaac ctttccactg gcaatttgca aaggagctgg
     5581 aaattgctgt cgcaggaaat atcattctgg atgatgttga tgctgaactg gaggccgttt
     5641 tgatgggtgc tggtatcggg tatctgctct atgaacagat caaggagtac cttgatactg
     5701 gtcgtttaga atgtgtactg ggagactgga gcacagagcg tccaggcttc cagatctatt
     5761 atccaaaccg ccagtatatg tcctgcggct tgcgcgcatt tctcgattat gtaaaaacgg
     5821 gtcagatctg ccagagtcag cgtcaccgac cacaataatt atttactatt cgctttacgc
     5881 gccagcagcg ggaaaatcag tcctaatcct accagcacaa acggcgttgc aacattcagc
     5941 gccaactgga aggtccactc tgcagtgaag gcttccattt tcgggaagat ccccgtcaaa
     6001 caggcaaagg cggtaaaggc aaaacaccaa atcccgacgg tcattgccag aggcttatta
     6061 cgaataaaga catattctgg tttatatttc tgcgccaggc gaacgactgc aataaatgca
     6121 acaaataccc ataaataacg cagcggcatc actaccgagt taagattcaa cagccattta
     6181 tagagattgt tcatatcacc gatgccgaga gtcggcagca taatcagaat tgccaccagc
     6241 accagggtca gaaaatagcc attaacgggc gtaccagaag cgttggtacg acataaactg
     6301 gctggaatat atttgctgtc agcatcacct aatagcactt taagcggggc atcaatcgag
     6361 aataccagcg cagctacttg tcccagggta ttcgcaatgg cgtaaatcac cattaaagta
     6421 ttacccatgt tgtaatactc gcccagcttc tgaaaggcgt aatattgacc gttagtcatt
     6481 aagtcatccg ggatattacg cgagtcaaac atcatcccca tcgccagtga gcccagaatg
     6541 gcacaaaccg caaccatcac cgccaggcat agcatccctt ttggaaattc ttttcctggg
     6601 ttgcgcgttt gattaacgta aggagaaatc ttctctgctc cgccaaccgc gaaaaccagc
     6661 attgaaatag tggtaatgta ggtaaagtcg atatgaggaa tgaacgtttc ccaggtaatg
     6721 tttgtggtcg caatatgcac ttcagtaatc gcagacgcgg ttaccgccat cgccacatac
     6781 aggagcgaca taacaaacat cgcaatccct gccacagaac cgacgatttt cagcgatttc
     6841 ataccgcgtg aagcaaccca cataaagaag ataaacagca ccagcgttaa cccctgtaac
     6901 gctacgactg aatattcttt gattagcgaa ccgtcgcctt tcatcgccca accgagcgca
     6961 atcagaattg cctggggttt ttgtgccaga aagggaatat gcaccaccca gtaggtccac
     7021 gcggcgagat aagccagtcc gggtcccatc gtgtgtttaa tccaggtact gaccccgccc
     7081 ttcccatctt tgaaggtcga gcctaactga ccaacaatta gcgcataagg tgtgaaatag
     7141 agtgcaaaga taaacaccca tgaaaaaaca accaccagcc cctggttggc atagttatta
     7201 acaacgttgc caaaacccca gacagtgata aaagacatca gcgcaatgtt gtaccatcgc
     7261 aactgttttt cctgaacatt cccagccata gacaaatcct tcgccaaaat taaaggggca
     7321 tctgtaaaac gagggattat gcgcggcaaa taagaagtgc ggaaatagcg aaatgcaaaa
     7381 ttataacctg ctgcacattt tatttttaaa cgaataaaat ttggaataaa gcaagtcaga
     7441 tagaagaaaa aaaaagcaga gtgtgaaaca gtaagttcac actctgcttg aattcattta
     7501 cacgtgagca acgggtttcg gcgtatgacc ggcgttgcgc agcagcatca gcaaatagat
     7561 aaacgatacg ctggcgatca tgataaacaa taaattatcg gagaaattct gcattagcat
     7621 agcggtaaat gacgggccaa gcagacttcc cacagtatag ctcaacagta aggcctggtt
     7681 catcgccacc agttgatgat gttcaacttt ctcgcaagcc catgccatcg ccaccggata
     7741 tagcgtaaag ccagcggcac cgaggatgaa taacgctggg gccatcgccg cctggctaag
     7801 catcgcgata ctgccgagaa tgacgacaaa gacctgaaca cgcaacacca gcagtcgacc
     7861 aaacttatcc gccagacgtc caatcggcca ttgtccaagg atacccgcac tgaccagtac
     7921 cgccatccag aaaccaatgc tggcattgct cacccctttg tgattgaggt acagcggcat
     7981 caggccatat agagatccca gaacgattcc tgagataatg cagccattca cgccaagccg
     8041 cgcctgacgg agttttagca ttgacgtaat tgacgtcgaa tcatggtttt cagcctgctg
     8101 attcagcaca cgcgtaaaca acagcggtaa gatccctgcc aacgtcaaac ctgtaaccca
     8161 cggcaatacg gacatcagct cggttgaaac tttgctgacc agtaactggc ctaaaaacgt
     8221 tcccacgtaa taaaccatca tatacgcagc aagcaaacgc ccacggttac gtgacgtccc
     8281 actgcacatc agcgcgctct caacaaccac ccaaatcatg gcacagccga cgcccgcgac
     8341 aaaacgccaa gccaaccagc tccagaatcc aatcatcagg ccaaggccgg cacagccagc
     8401 ggcaaaaatg aaggaggcca gataatagct gcggttaaag ccaatgcgct taatgacata
     8461 ccctgtcagc aatgtaccga caaggttgcc ggtaaaatag gatgagctga caacgcctac
     8521 ctgccatgtg gacatgtgtt cctgggcgag ccaaagcggc acgagtgtat ttaacaccgc
     8581 gatcgccaga gtcaacaaaa gcaggccaga cagcaaaagc atgacaggcc gggtatacgt
     8641 ggacatgaat taaaaccgtg aggaagttca aatttcatgc gcatcatgcc accgccaaaa
     8701 acattgtcaa tgggtgaaaa tacgcgcata tcgcagcggc ggtattatcg atttatatta
     8761 ctcattcata acgctaatgc tgaagagatg attaatctca tctgattgtt tttattgatc
     8821 taacacaaat ttcatacgct tcgttcagtc gaaaaatttc atggtcacag caaactcata
     8881 tttcccgtct atgctttcag agcaatgcat cacgtctgac cgatttcaga cgtaccactg
     8941 actctcgaaa ggagaagagg atgaccaaac cgtatgttcg tcttgataaa aatgatgctg
     9001 ccgttttgct tgttgatcat caggctggtt tactttccct tgtacgggat atcgaacccg
     9061 ataagtttaa aaataacgtg ctggcgctgg gtgacctggc taagtatttc aacttaccta
     9121 ctattcttac caccagtttt gaaaccggtc ctaacggtcc attagttcca gaactgaaag
     9181 cacaattccc cgatgcacct tacattgctc gcccgggaaa tattaacgcc tgggataacg
     9241 aagattttgt aaaagcagtc aaagcgacag gtaaaaaaca gttaattatt gccggtgtgg
     9301 taaccgaagt ttgcgtggca ttcccggcgc tttcagccat tgaagaaggg tttgatgtct
     9361 ttgttgtgac tgacgcgtca gggaccttta atgaaattac ccggcattcg gcatgggatc
     9421 gcatgtcgca agcaggtgcg caattgatga catggttcgg cgtggcgtgc gagctgcacc
     9481 gtgactggcg taatgatatt gaaggactgg cgacgttgtt ctctaaccat attcctgatt
     9541 atcgtaattt gatgaccagt tatgacacgt taacgaagca gaaataaaag agaaccgggg
     9601 ccgaaacccc ggttgttact gattagcttg cgacggccat ccccacggtc atatgcaaac
     9661 catagaatac gccgcgaccg attaattccc ctgccagaag cagaatgaat gagacggata
     9721 gtagcggaac cgcaggctga taacctttta gctgtggtgc aatccacaag cacagggcaa
     9781 cggccaaaag cacgatccgc caggacatca gcgcaccata gtccggcacc agcgctgcgg
     9841 cctgctgcac agaactatga atagttgcca gctctgtgcc ctgcatcact gacatcacgc
     9901 cactcaccac cagtgccagt acagaaatgg ctggcagcag acgcatcgcc cagccatcga
     9961 cgccagccag actcaacaac aggtaaccca gtaacgggcc gcccataaac atcgtcagga
    10021 agaagcccat cggcgtccag atgctgtacc aggtcggaac ggtatcaatg ctgttataca
    10081 cacgcaccat catccagaca aagatgacgc caagaaccat cgtcactatc agccacagcg
    10141 tacgcaatgc cggtgacagc tttttcagca ttgccagcaa ccagccgatg ccgcctacgg
    10201 caaaaaagat cgaaccgctg gcgatttcgt tactgagtgc tgaagcccct acccggttga
    10261 gcgagttaaa agcgcgcatt ggtgaaccaa gatggagcat agaggcgata aagccaatgc
    10321 ccatcagcac ccataaacca aacatgcagg cgataacacg ctgctgggct tctgctcgca
    10381 ggtcgccttt gagcagcgcc aaagccagaa cgataaaacc acctgctaca cattgcccga
    10441 agaccgtgaa gatcatcagc ggccattcat gccatccact tcccatctca cacctccttc
    10501 gggtttgcca gatagccagt ggtatccccg gtcgggcggc tattggcatt gggtttgatc
    10561 acaatattcg gtttggtaaa gtgagctcgc ggcaacggcg caacggccgc cagatcgcca
    10621 tgttttttac gcagctcgtc gatagggccg aaatccagcg cccgcagcgg acaagattca
    10681 acacagatcg gctttttacc ctccgcaaca cggtcataac aaccatcgca tttggtcata
    10741 tggcctttcg tttcgttata ttgcggtgcg ccatacgggc aagccatatg gcagtagcgg
    10801 cagccaatgc acacatcttc atcgaccaca acaaaaccat cttcacgttt atgcatcgca
    10861 ccgctcgggc agactttagt acaagccgga tcttcgcagt ggttacatga aatcgacaga
    10921 tagtaggcaa acacgttctg gtgccagaca ccgttatctt cctgccagtc gccgccagca
    10981 tattcataaa tgcggcggaa gctgacttct ggcgtcaaat ctttgtagtc tttacaggcc
    11041 agctcgcagg ttttgcaacc ggtgcaacgg ctggaatcaa taaaaaatcc atactgggtt
    11101 gtcatcggtt actccttaca ccttttcaac ctgaacaagg tttgtatgtg acggattccc
    11161 cttagcgaga ggagacggac gttgagtggt cagtacgttg atacaaccgc ccttatcgac
    11221 acgttttgta tccgggtcat accaggcacc ttcacccagt gcgaccacac ccggcatcat
    11281 tcgtggcgtc actttcgcct caatatgaac ctcaccacga tcgttaaaga tcctgacttt
    11341 atcgccgttg ttgataccgc gtttttgcgc atcaagcggg ttgatccaca tttcctgacg
    11401 gcaagctgct ttcagcacat caacgttgcc gtaagttgag tgaacgcgag atttatagtg
    11461 gaaacccgta agctgcagcg gatactgttt gttcagcgga tcctgataac tttcaaagcc
    11521 cggcgtgtag atcggcagtg gatcgatcac atcgccttct ggcaattccc aggtagcggc
    11581 aatgtcagcc agcgcctgcg aataaatctc aattttgccc gatggcgtag tcagtgggtt
    11641 tgcctgcgga tcttcacgga aggctttata agcaacgtga tgcccttgtg ggtcgcgctt
    11701 tttaaagatc ccctgcttgc ggaactcttc aaacgttggc agttcaggaa tcgcttcccg
    11761 cgactgggca tacagatgcc gcatccactc ttcctgggta cggccttcag taaactgttg
    11821 ctcaacgcca agacgttttg ccagttcgct ggtcatttca tagatggtct tacattcaaa
    11881 gcgcggttta atcacctgat cgttgaaaat cacgtaagac atattcccgc aggatgcatc
    11941 cagtgcaaag tccatctgtt cggaagcggt gcagtcaggc agcaggatgt cagcatattt
    12001 cgccgatgag gtcatgtggc agtcgataac cacaatcagc tcgcacttct tatcatcctg
    12061 aaggatttca tgggtacggt tgatttcaga atgctggtta atcaggcagt taccggcata
    12121 gttccagatc attttgatcg gcacatccag cttatctttc ccgcgtacac catcacgcag
    12181 cgccgtcatt tccgggccac gttcaatggc atcggtccac ataaacatcg aaatgctggt
    12241 ctggatcggg ttttccaagg tcggcatacg gacaaacggt aagctgtatg aaccttcacg
    12301 cgcgccgctg ttgcctccgt taataccaac gttaccggtc agaatcgcca gcatcgagat
    12361 agcacgggtt gcgatttcac cgttagcgtg acgctgcggg ccccatccct ggctgataaa
    12421 cgccggtttg gtactgccga tttcacgagc cagtttgatg attttgtctg ccggaacacc
    12481 ggtgatttgt gaggcccatt ccggtgtttt agccacgcca tctggccctt cacccagaat
    12541 ataagcttta tagtggccat ttttcggcgc actggctggc agggttttct catcgtagcc
    12601 aacgcaatat ttatcgagga atgcctgatc caccaggttt tcagtgatca tgacgtacgc
    12661 cagaccgtta accagtgcgg catctgttcc cggacgaata gggatccact catcttcgcg
    12721 cccggcaccg gtgtcggtat agcgcggatc aatgatgatc atgcgggcat tagatttctg
    12781 gcgtgcctgt tcaagatagt aagtcacccc accgccactc attcgcgttt cgccagggtt
    12841 attaccaaac agcactacca gcttactgtt ttcgatatcc gacgggctgt tgccatctgc
    12901 ccagccgccg taggtatagt tcaaaccttc cgcaatttgc gcggaggagt agtcgccgta
    12961 atggttcaga tagccgccgc agcagttcat cagccgcgcg accagggtat ttcccggcgg
    13021 ccaggagcgg gtcatggtgc cgcccagcgt accggtgcca tagttcagat agatagactc
    13081 gttgccgtac tctttgatca ggcgctgcat attggtcgcg atgatgtcgt aggcttcttc
    13141 ccagctaatg cgctcgaatt tgccttcacc gcgcgccccg actcgtttca tcggatattt
    13201 caggcggtcc ggattgtaga cacggcgacg catggaacgc ccacgcaggc aggcgcgaac
    13261 ctggtgcagg ccgtcgtaat tgtcatcgcc ggtattgtcc gtttcgacat atttgatttc
    13321 accgtccacg acgtgcatac gtagcgggca gcgactacca cagttaactg tacaggcgct
    13381 ccagataacc ttttcgtctg attttgttgg aatggcgcta tcgacagcgt gcgcaatccg
    13441 actaaaaggt aatgttaatg cgctgctggc cattgccagg ccgccgatcg ctgtcgtttt
    13501 taccaaacca cggcgactca cctcagcagc caataccgca tcagggattt tcgttttcat
    13561 aatggctcac tcaagcttgc tcacgattga ttccagtaac aaaccgtatt gtgtaattat
    13621 ttctatagca gctc
//
LOCUS       DAZFWL020000126        13181 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFWL020000126 DAZFWL020000000
VERSION     DAZFWL020000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.126"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            268..1377
                     /gene="livK"
                     /locus_tag="ACHKNP_RS19360"
                     /old_locus_tag="pgaptmp_003872"
     CDS             268..1377
                     /gene="livK"
                     /locus_tag="ACHKNP_RS19360"
                     /old_locus_tag="pgaptmp_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HGU4705956.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1425..2351
                     /gene="livH"
                     /locus_tag="ACHKNP_RS19365"
                     /old_locus_tag="pgaptmp_003873"
     CDS             1425..2351
                     /gene="livH"
                     /locus_tag="ACHKNP_RS19365"
                     /old_locus_tag="pgaptmp_003873"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HGU4705957.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2348..3625
                     /gene="livM"
                     /locus_tag="ACHKNP_RS19370"
                     /old_locus_tag="pgaptmp_003874"
     CDS             2348..3625
                     /gene="livM"
                     /locus_tag="ACHKNP_RS19370"
                     /old_locus_tag="pgaptmp_003874"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HGU4705958.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3622..4389
                     /gene="livG"
                     /locus_tag="ACHKNP_RS19375"
                     /old_locus_tag="pgaptmp_003875"
     CDS             3622..4389
                     /gene="livG"
                     /locus_tag="ACHKNP_RS19375"
                     /old_locus_tag="pgaptmp_003875"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HGU4705959.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4391..5104
                     /gene="livF"
                     /locus_tag="ACHKNP_RS19380"
                     /old_locus_tag="pgaptmp_003876"
     CDS             4391..5104
                     /gene="livF"
                     /locus_tag="ACHKNP_RS19380"
                     /old_locus_tag="pgaptmp_003876"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HGU4705960.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5500..6816
                     /gene="ugpB"
                     /locus_tag="ACHKNP_RS19385"
                     /old_locus_tag="pgaptmp_003877"
     CDS             5500..6816
                     /gene="ugpB"
                     /locus_tag="ACHKNP_RS19385"
                     /old_locus_tag="pgaptmp_003877"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HGU4705961.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6914..7801
                     /gene="ugpA"
                     /locus_tag="ACHKNP_RS19390"
                     /old_locus_tag="pgaptmp_003878"
     CDS             6914..7801
                     /gene="ugpA"
                     /locus_tag="ACHKNP_RS19390"
                     /old_locus_tag="pgaptmp_003878"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HGU4705962.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7798..8643
                     /gene="ugpE"
                     /locus_tag="ACHKNP_RS19395"
                     /old_locus_tag="pgaptmp_003879"
     CDS             7798..8643
                     /gene="ugpE"
                     /locus_tag="ACHKNP_RS19395"
                     /old_locus_tag="pgaptmp_003879"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HGU4705963.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8645..9715
                     /gene="ugpC"
                     /locus_tag="ACHKNP_RS19400"
                     /old_locus_tag="pgaptmp_003880"
     CDS             8645..9715
                     /gene="ugpC"
                     /locus_tag="ACHKNP_RS19400"
                     /old_locus_tag="pgaptmp_003880"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HGU4705964.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9712..10455
                     /gene="ugpQ"
                     /locus_tag="ACHKNP_RS19405"
                     /old_locus_tag="pgaptmp_003881"
     CDS             9712..10455
                     /gene="ugpQ"
                     /locus_tag="ACHKNP_RS19405"
                     /old_locus_tag="pgaptmp_003881"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU4705965.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLWILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10442..10882)
                     /locus_tag="ACHKNP_RS19410"
                     /old_locus_tag="pgaptmp_003882"
     CDS             complement(10442..10882)
                     /locus_tag="ACHKNP_RS19410"
                     /old_locus_tag="pgaptmp_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HGU4705966.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            11002..12744
                     /gene="ggt"
                     /locus_tag="ACHKNP_RS19415"
                     /old_locus_tag="pgaptmp_003883"
     CDS             11002..12744
                     /gene="ggt"
                     /locus_tag="ACHKNP_RS19415"
                     /old_locus_tag="pgaptmp_003883"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HGU4705967.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     VSDPRSVDDLTAGY"
     gene            complement(12782..13066)
                     /locus_tag="ACHKNP_RS19420"
                     /old_locus_tag="pgaptmp_003884"
     CDS             complement(12782..13066)
                     /locus_tag="ACHKNP_RS19420"
                     /old_locus_tag="pgaptmp_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HGU4705968.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2928 a         3351 c         3766 g         3136 t
ORIGIN      
        1 ccctgaatgg tagtgaatta ttccgccctt tgtgccgtta ttttatgctg acaaaggcac
       61 ttttttctgt ttatctatca ataaattcag aatattatct gttcttaatc gactgaaaaa
      121 tagggatttt aatcactatt atcacaaaat actgcgctaa acccttaatc agacaggcaa
      181 aaacagtgca gtataaaaaa agaacagtct gatttgttaa cacataaaaa caaagcaaca
      241 caacatcacg aatggggatt tttgactatg aaacggaatg cgaaaactat catcgcaggg
      301 atgattgcac tggcaatttc acacaccgct atggctgacg atattaaagt cgccgttgtc
      361 ggcgcgatgt ccggcccgat tgcccagtgg ggcgatatgg aatttaacgg cgcgcgtcag
      421 gcgattaaag acattaatgc caaaggggga attaagggcg acaagctggt tggcgtggaa
      481 tatgacgacg cctgcgaccc gaaacaagcc gttgcggtcg ccaacaaaat cgttaatgac
      541 ggcattaaat acgttattgg tcatctgtgt tcttcttcta cccagcctgc gtcagatatc
      601 tatgaagacg aaggtattct gatgatctcg ccgggagcga ccaacccgga gctgacccaa
      661 cgcggttatc aacacattat gcgtactgcc gggctggact cttcccaggg gccaacggcg
      721 gcaaaataca ttcttgagac ggtgaagccc cagcgcatcg ccatcattca cgacaaacaa
      781 caatatggcg aagggctggc acgttcggtg caggacgggc tgaaagctgc taacgccaat
      841 gttgttttct tcgacggcat taccgctggc gagaaagatt tctccgcgct gatcgcccgc
      901 ctgaaaaaag aaaacatcga cttcgtttac tacggcggtt actacccgga aatggggcag
      961 atgctgcgcc aggcccgttc cgttggcctg aaaacccagt ttatggggcc ggaaggtgtg
     1021 ggtaatgcgt cgctgtcgaa tattgctggc gatgctgccg aaggcatgtt ggtcaccatg
     1081 ccaaaacgct atgaccagga tccggcaaac cagggcatcg ttgatgcgct gaaagcagac
     1141 aagaaagatc cgtccgggcc ttatgtctgg atcacttacg cggcggtgca atctctggca
     1201 acagcccttg agcgtaccgg cagcgatgag ccgctggcgc tggtgaaaga tttaaaagct
     1261 aacggtgcaa acaccgtgat tgggccgctg aactgggatg aaaaaggcga tcttaaggga
     1321 tttgattttg gtgtcttcca gtggcacgcc gacggttcat ccacggcagc caagtgatca
     1381 tcccaccgcc cgtaaaatgc gggcgggttt agaaaggtta ccttatgtct gagcagtttt
     1441 tgtatttctt gcagcagatg tttaacggcg tcacgctggg cagtacctac gcgctgatag
     1501 ccatcggcta caccatggtt tacggcatta tcggcatgat aaacttcgcc cacggcgagg
     1561 tttatatgat tggcagctac gtctcattta tgatcatcgc cgcgctgatg atgatgggca
     1621 ttgataccgg ctggctgctg gtggcggcgg gattcgtcgg cgcaatcgtc attgccagcg
     1681 cctacggctg gagtatcgaa cgggtggcct atcgcccggt gcgtaactct aagcgcctga
     1741 ttgcgctcat ctcggcaatc ggtatgtcca tcttcctgca aaactacgtc agcctgaccg
     1801 aaggttcgcg tgacgtggcg ctgccgagcc tgtttaacgg tcagtgggtg gtgggccata
     1861 gcgaaaactt ctctgcctct attactacca tgcaggcggt gatctggatt gtcaccttcc
     1921 tcgccatgct ggcgctgacg attttcattc gctattcccg tatggggcgc gcgtgtcgtg
     1981 cctgcgcgga agatctgaaa atggcgagtc tgcttggcat taacaccgac cgggtgattg
     2041 cgctgacctt tgtgattggc gcggcgatgg cggcggtggc gggtgtgctg ctcggtcagt
     2101 tctacggcgt aattaacccc tacatcggct ttatggccgg gatgaaagcc tttaccgctg
     2161 cggtgctcgg tgggattggc agcattccag gggcgatgat tggtgggctg atcctgggga
     2221 ttgcggaggc gctctcttct gcctatctga gtacggaata taaggatgtg gtttcatttg
     2281 ccctgctgat tctggtgctg ctggtgatgc cgaccggtat tctgggtcgc ccggaggtag
     2341 agaaagtatg aaaccgatgc atattgcaat ggcgctgctc tctgccgcga tgttttttgt
     2401 cctggcgggc gtctttatgg gcgtgcaact ggagctggat ggcaccaaac tggtggtcga
     2461 cacggcttca gacatccgtt ggcagtgggt gtttatcggc acggcggtgg tctttttctt
     2521 ccagcttttg cgaccggctt tccagaaagg gctaaaaagc gtttccggac cgaagtttat
     2581 tctgcccgcg attgatggct ccacggtgaa gcagaaacta ttcctcgtgg cgctgctggt
     2641 gcttgcggtg gcgtggccgt ttatggtgtc gcgtgggacg gtggatatcg ccaccctgac
     2701 catgatctac attatcctcg gcctcgggct gaacgtggtt gttggtcttt ctggtctgct
     2761 ggtgctgggg tacggcggct tttacgccat cggcgcttac acttttgcgc tgctcaatca
     2821 ctattacggc ctgggcttct ggacctgtct gccgattgcc gggttaatgg cagcggcggc
     2881 cggcttcctg ctcggcttcc cggtgcttcg cttgcgcggc gactatctgg cgatcgttac
     2941 cctcggtttc ggcgaaattg tgcgcatatt gctgctcaat aacaccgaaa ttaccggcgg
     3001 cccgaacgga atcagtcaga tcccgaaacc gacactcttc ggactcgagt tcagccgtac
     3061 cgctcgtgaa ggcggctggg acacgttcag taatttcttt ggcctgaaat acgatccctc
     3121 cgatcgtgtc atcttcctct acctggtggc gttgctgctg gtggtgctaa gcctgtttgt
     3181 cattaaccgc ctgctgcgga tgccgctggg gcgtgcgtgg gaagcgttgc gtgaagacga
     3241 gatcgcctgc cgttcgctgg gcttaagccc gcgtcgtatc aagctgactg cctttaccat
     3301 aagtgccgcg tttgccggtt ttgccggaac gctgtttgct gcgcgccagg gctttgtcag
     3361 cccggaatcc ttcacctttg ctgaatcggc gtttgtactg gcgatagtgg tgctcggcgg
     3421 tatgggatcg cagtttgcgg tgattctggc ggcaattttg ctggtggtat cgcgcgaatt
     3481 gatgcgtgat ttcaacgaat acagcatgtt aatgctcggt ggtttgatgg tgctgatgat
     3541 gatctggcgt ccgcagggct tgctgcccat gacgcgcccg caactgaagc tgaaaaacgg
     3601 cgcagcgaaa ggagagcagg catgagtcag ccattattat ctgttaacgg tctgatgatg
     3661 cgcttcggcg gcttgctggc ggtgaacaac gtcaatcttg aactgtaccc gcaggagatc
     3721 gtctcgttaa tcggccctaa cggtgccgga aaaaccacgg tttttaactg cctgaccgga
     3781 ttctacaaac ccaccggcgg cactatttta ctgcgtgatc agcatctgga aggtttaccg
     3841 gggcagcaaa ttgcccgcat gggcgtggtg cgtaccttcc agcatgtgcg tctgttccgt
     3901 gaaatgacgg tgattgaaaa cctgctggtg gcgcagcatc aacaactgaa aaccgggctg
     3961 ttctctggcc tgttgaaaac gccttctttc cgtcgcgccc agagcgaagc gctcgaccgc
     4021 gccgccacct ggcttgagcg cattggtttg ctggaacacg ccaaccgtca ggcgagtaac
     4081 ctggcctatg gtgaccaacg ccgtctggag attgcccgct gcatggtgac gcagccggag
     4141 attttaatgc tcgacgaacc ggcggcaggt cttaacccaa aagaaaccaa agagctggat
     4201 gagctgattg ccgaactgcg caatcatcac aacaccacta tcttgttgat tgaacacgat
     4261 atgaagctgg tgatggggat ttcagaccga atttatgtgg tcaatcaggg gacgccactg
     4321 gcaaacggta cgccggagca aatccgtaat aacccggacg tgatccgtgc ctatttaggt
     4381 gaggcataag atggaaaaag tcatgttgtc ctttgacaaa gtcagcgccc actacggcaa
     4441 aatccaggcg ctgcatgagg tgagcctgca tatcaatcag ggcgagattg tcaccctcat
     4501 tggcgcgaac ggggcgggga aaaccacctt gctcggcacg ttatgcggcg atccgcgcgc
     4561 taccagcggg cgaattgtgt ttgatgataa agacattacc gactggcaga cggcgaaaat
     4621 catgcgcgaa gcggtagcga ttgtcccgga agggcgtcgc gtcttctcgc ggatgacggt
     4681 ggaagagaac ctggcgatgg gcggcttttt tgccgaacgc gaccagttcc aggagcgcat
     4741 aaagtgggtg tatgaactgt ttccacgtct gcatgagcgc cgtgttcagc gggcaggcac
     4801 catgtccggc ggtgagcaac aaatgctggc gattggtcgc gcgctgatga gcaacccgcg
     4861 tttgctgcta cttgatgagc catcgctcgg tcttgcgccg attatcatcc agcaaatttt
     4921 cgacaccatc gagcagctgc gcgagcaggg gatgactatc ttcctcgtcg agcagaacgc
     4981 caaccaggcg ctaaagctgg cggatcgcgg ctacgtgctg gaaaacggcc atgtggtgct
     5041 ctccgatacc ggcgacgcgc tgctggcgaa cgaagcagtg agaagtgcgt atttaggcgg
     5101 gtaataacac gttgattgat aaggagtcaa aagactcctt tgagacaggt gacaaatgta
     5161 aaattgcctg atgcgctgcg cttatcaggc ctactgggtg agttgcaata tattgaattt
     5221 gcacgatctt gtaggccgga taaggcgttt acgccgcatc cggcatgaaa cgatgggtaa
     5281 tctgtagagt ttgattcaga ccttctattt tccgcttatc cgtgccccat ctcccatttt
     5341 ccctcaccca cgccgtcacc gccttgtcat ctttctgaca ccttactatc ttacaaatgt
     5401 aacaaaaaag ttatttttct gtaattcgag catgtcatgt taccccgcga gcataaaacg
     5461 cgtgaattcg cgcattcggt acaacaagag agataaacga tgaaaccgtt acattataca
     5521 gcttcagcac tggcgctcgg actggcgtta atggggaatg cacaggcagt gacgaccatt
     5581 ccgttctggc attctatgga aggggaactg ggtaaagagg tggattctct ggcccaacgt
     5641 tttaacgccg aaaatccgga ttacaaaatt gtaccgacct ataaaggcaa ctacgaacag
     5701 aatttaagcg cggggattgc cgcatttcgt accggcaacg ctccggctat tttgcaggtt
     5761 tatgaagttg gcaccgccac catgatggca tcgaaagcca ttaaaccggt atatgacgtg
     5821 tttaaagagg cggggattca attcgatgag tcgcagtttg tgccgacggt ttccggttac
     5881 tactccgaca gcaaaacggg ccacttactc tcccagccat tcaacagctc gacccccgtt
     5941 ctctattaca acaaagacgc cttcaagaaa gcaggattag acccggaaca accgccgaaa
     6001 acctggcagg atctggcgga ctatgccgcg aaactgaaag cctccggtat gaagtgcggc
     6061 tacgccagcg gctggcaggg ctggatccaa ctggaaaact ttagcgcctg gaacggtctg
     6121 ccgtttgcca gcaaaaacaa cggctttgac ggcacggacg cggtgctgga gttcaacaag
     6181 ccggagcagg tgaaacatat cgccatgctc gaggagatga acaagaaggg cgacttcagc
     6241 tacgtcggtc gtaaggatga atccaccgag aagttctata acggtgattg cgcgatgacc
     6301 accgcctctt ccggttctct tgccaacatt cgcgagtacg ccaaatttaa ttacggcgta
     6361 ggcatgatgc cttacgacgc cgatgcgaaa gatgcgccac aaaacgccat tatcggcgga
     6421 gccagcctgt gggtgatgca gggtaaagat aaagaaacgt ataccggtgt ggcgaagttc
     6481 ctcgatttcc tcgcgaagcc agaaaacgct gccgagtggc atcagaaaac cggttatctg
     6541 ccaattacca aagcggcgta tgacctgacc cgtgagcagg gcttttacga gaaaaaccca
     6601 ggtgcggata ccgcgacgcg tcagatgctg aataagccgc cgttgccgtt caccaaaggg
     6661 ctgcgtctgg gcaacatgcc gcagatccgc gtgattgtgg atgaagagct ggagagcgtg
     6721 tggaccggta agaagacacc acagcaggca ctggataccg ccgttgagcg tggaaatcag
     6781 ttgctgcgcc gctttgagaa atcgacgaag tcttaatcag tgtaatgtcg gatgcgtttc
     6841 gcttatctga cttggcatcg cgtgtaggcc ggataagcga agcgcatccg gcacagttca
     6901 ggaattaacc gtaatgtcat catcccgtcc ggtgttccgc tcgcgctggc tgccttatct
     6961 gctggtcgcg ccgcagctca tcatcaccgt tatctttttt atctggcctg cgggcgaagc
     7021 gttgtggtac tcgctacaaa gcgtcgatcc gtttggtttc tccagccagt ttgtcggcct
     7081 ggataacttc gtcgcgctgt ttcatgacag ctactatctc gactccttct ggacgacgat
     7141 aaaattcagc acctttgtca ccgtcagcgg tttgctggtg tcgctgttct tcgcggcgct
     7201 ggtggagtac atcgtgcgcg gcagccgttt ctatcaaacc ttaatgctgc tgccttacgc
     7261 cgtggctccc gccgttgccg ccgtattgtg gatcttcctg tttaaccccg gtcgcgggct
     7321 gatcacccat tttctcgcgg agttcggcta cgactggaac cacgcgcaaa acagcggtca
     7381 ggcaatgttt ctggtggtgt ttgcctcagt atggaagcaa atcagctaca acttcctgtt
     7441 cttctatgcc gcgctgcaat ccattccccg ttcgttgatc gaagccgcag ccatcgacgg
     7501 tgcaggccca attcgccgct tctttaagat tgcgctgccg cttatcgctc cggtgagttt
     7561 cttcctgctg gtggtgaacc tggtgtatgc cttcttcgac accttcccgg tgatcgacgc
     7621 cgccacgtcc ggcgggccgg ttcaggccac cacgacgctg atttataaga tctaccgtga
     7681 aggctttacc ggactggatc tggcttcgtc tgccgcgcag tcggtggtgt tgatgttcct
     7741 cgtcatcgtg ctgacggtgg tgcagttccg ctatgttgaa agcaaggtgc gttaccaatg
     7801 attgagaacc gtccgtggct gacgatattc agccatacca tgctgatcct cgggatcgcg
     7861 gtgatcctct tcccgctgta cgtggcgttt gtcgcggcga cgctggataa acaggccgtc
     7921 tatgccgcgc cgatgacgct catccccggc acacatctgc tggaaaatat ccacaacatc
     7981 tgggttaacg gggtaggcac gaatagcgcg ccgttctggc ggatgttgct taacagcttt
     8041 gtgatggcgt tcagcattac gctcggcaaa attaccgtct cgatgctctc ggcatttgcc
     8101 attgtctggt ttcgttttcc gctacgtaac ctcttcttct ggatgatttt tatcaccctg
     8161 atgctgccgg ttgaagtacg tatcttcccg acggtggaag tcatcgccaa cctgaagatg
     8221 ctcgacagct acgccggttt aacgctgccg ctgatggcct cggcgaccgc cacgtttctg
     8281 tttcgccagt tctttatgac gctgccggat gaactggtgg aagcggcgcg gatcgacggc
     8341 gcgtcaccaa tgcgtttctt ttgcgacatc gtttttccgc tctccaaaac caatctggcg
     8401 gcgctgtttg tgatcacctt tatttacggc tggaaccagt atttgtggcc gttgttgatt
     8461 attaccgatg tggatctcgg caccaccgtg gcagggatca aagggatgat cgctacaggc
     8521 gaaggcacca ctgaatggaa ctcagtgatg gcggcgatgt tgttaacgct tatccctccg
     8581 gtggtgattg ttttagtgat gcagcgtgcc ttcgtgcgcg gcctggtcga tagtgagaaa
     8641 taagatggca ggactgaaat tacaggcagt aaccaaaagc tgggatggta aaacccaggt
     8701 cattaaaccg ctgacccttg atgtggcgga tggcgaattt atcgtgatgg tcgggccgtc
     8761 tggctgcggg aaatcgacgc tgctgcgcat ggttgccggg ctggagcggg tgacagaagg
     8821 cgatatctgg atcaacgacc agcgcgtgac tgaaatggag ccaaaagatc gcgggattgc
     8881 gatggtgttc cagaactacg cgctttatcc gcatatgagt gtcgaagaaa acatggcgtg
     8941 ggggctgaaa attcgcggca tgggcaagca gcaaattgcc gagcgcgtta aagaagcggc
     9001 gcgcattctg gagctggacg gtctgctcaa acgtcgcccg cgcgagcttt ccggcggtca
     9061 gcgccagcgt gtggcgatgg gccgcgcgat tgtgcgcgat ccggcggtgt tcctgtttga
     9121 tgagccgctc tctaacctcg atgccaagct gcgcgtgcag atgcgtcttg aactgcaaca
     9181 gttgcaccgt cgcctgaaaa cgacttcact ctacgttact cacgatcagg ttgaagcgat
     9241 gacgctcgcc cagcgagtaa tggtgatgaa cggcggcgtt gccgaacaga ttggcacacc
     9301 agttgaagtc tacgaaaagc ccgccagcct gtttgtagcg agttttatcg gcagtccggc
     9361 gatgaacctg ctgacaggcc gcgtgaataa cgaaggcacg catttcgaac tggacggcgg
     9421 tattgagctg ccgctaaacg gtggctaccg tcagtatgcc gggcgtaaaa tgactctcgg
     9481 cattcgcccg gaacatattg cgctaagctc gcaggcagaa ggcggcgtac cgctggtgat
     9541 ggacacgctg gagatcctcg gcgcagataa cctggcgcac ggacgctggg gcgagcagaa
     9601 gctggtggtg cgactggcgc atcaggagcg cccgacggca ggcagcacgc tgtggctgca
     9661 tctggcggaa aatcagctgc atctttttga tggtgaaaca ggacaacgag tatgagtaac
     9721 tggccttatc cccgcatcgt cgctcatcgt ggcggcggta agctggcccc ggaaaacacc
     9781 ctggcggcaa tcgacgtcgg ggcaaaatac ggtcataaga tgatcgaatt tgacgcgaag
     9841 ttatcgaaag atggcgagat cttcctgctc catgacgaca atctcgaacg caccagcaac
     9901 ggctggggcg tcgcgggtga actgaactgg caggatttac tgcgcgtgga tgcgggcagt
     9961 tggtacagca aagcgtttaa aggtgagccg ctgccgttgc tttcgcaggt ggcggaacgc
    10021 tgtcgcgaac acgggatgat ggcgaatatc gaaatcaaac ccaccactgg cactggacca
    10081 ttaacgggca aaatggtggc gctggcggca cgccaactgt gggccggtat gacgccgccg
    10141 ctgctgtcat cgtttgagat tgatgcttta gaagctgcac agctggcggc accggaactg
    10201 ccgcgtggtt tattgcttga tgagtggcgc gacgactggc gcgaactgac cgcgcggctg
    10261 ggctgcgtct ctattcatct caatcataag ttactcgata aagtgcgagt gatgcagttg
    10321 aaagacgccg gactgtggat tctggtttat accgtcaata aaccccagcg cgcggcagag
    10381 ttgctgcgct ggggcgtgga ttgcatctgt accgatgcga ttgacgtgat tggtccgaac
    10441 tttacggccc aatagttttc aacggaatgt caggctgcgg cgtgctgggc tgattcagca
    10501 tgtcgccgtt tctcctctcc ggcagcatat gctgctgatt caacgaacta tccggattac
    10561 ggttgctgtt taacatcccg ccgttggtgt tgggcagcat ttgctgccgg gcgggatttc
    10621 gctcccccgg ctgtgactgc aacacccgct gagaattatt gtttatctgg ttttctaaat
    10681 gctgctgttg caactgagtt tgcgttttca gttgctgatt cagcatccct ttttgctgga
    10741 tttgctgagt ctgcatctgg gtttgcatcc gctgctggct gggtatctga taccccggct
    10801 ggttagggtt gttcagagta ttaatgggct gtgcaaagcc gacaaacggc aggagtgccg
    10861 taagaatcag aagccgtttc atcgcgtatc ctcctctgaa gatatccttt aagtttactc
    10921 gcttcccgac aaaacgatga ttaattcaga gttatatacc aggcttagct ggggttgccc
    10981 cttaatctct ggagaataac gatgataaaa ccgacgtttt tacgccgggt ggccattgct
    11041 gctctgctct caggaagttg ttttagcgcc gccgccgcgc ctcctgcgcc gcccgtctcg
    11101 tatggtgtgg aggaagatgt cttccacccg gtacgcgcga aacagggaat ggtagcgtct
    11161 gtggacgcca ctgccactca ggtgggggtg gatattctca aggagggcgg gaatgccgtt
    11221 gatgccgccg tggcggtggg ctacgcgctg gcggtaacgc atccgcaggc agggaatctg
    11281 ggcggtggtg gttttatgct gatccgctcg aaaaatggca ataccaccgc tatcgatttc
    11341 cgcgaaatgg cacccgctaa agcgacccgc gatatgttcc ttgatgacca gggcaacccg
    11401 gacagcaaaa aatcactcac ttcgcatctg gcttccggca caccaggtac ggtcgctggt
    11461 ttctcgctgg cgttggataa gtacggcact atgccgttga acaaagtggt gcagcccgcg
    11521 ttcaaactgg cacgcgatgg ttttatcgtt aacgatgctt tggcagacga tctcaaaacc
    11581 tacggtagcg aagtgttgcc gaatcacgaa aacagcaaag ctatcttctg gaaagagggc
    11641 gaaccgctga aaaagggcga cacgctggtg caggcgaacc tggcaaagag cctggagatg
    11701 attgctgaaa acggcccgga cgaattctat aaaggcacga ttgcggaaca gatcgcccag
    11761 gagatgcaga aaaacggtgg cttgatcact aaagaagatt tagcggccta taaagcggtc
    11821 gaacgcactc cgataagcgg cgattatcgc gggtatcagg tttactccat gccaccgcca
    11881 tcctccggcg ggatccatat cgtacaaatc ctcaatattc tggaaaactt cgatatgaag
    11941 aaatacggct ttggcagcgc cgatgcgatg caaatcatgg cagaagcgga gaaatacgcc
    12001 tacgccgacc gctcggaata tcttggcgac ccggattttg tcaaagtccc gtggcaggca
    12061 ctgaccaata aagcctatgc caaatccatt gccgatcaaa ttgatatcaa caaagcgaag
    12121 ccgtccagtg agattcgccc tggcaagctt gcgccttatg agagtaatca aactacccat
    12181 tactctgtag tggataaaga cggtaacgcg gtggcggtga cctatacgct gaacaccacc
    12241 ttcggtacgg gtattgtcgc gggcgagagc ggtattctgc ttaataacca gatggatgat
    12301 ttctccgcca aaccgggcgt accgaacgtt tacgggctgg tgggcggtga tgccaacgcc
    12361 gtcgggccga acaaacgccc gctgtcgtcg atgtcgccga ccattgtggt gaaagacggt
    12421 aaaacctggc tggttaccgg tagcccaggc ggtagccgga tcatcactac agtgctgcaa
    12481 atggtggtga atagcatcga ttatggcatg aacgtcgccg aagcgaccaa tgcgccgcgt
    12541 ttccaccatc agtggttgcc ggacgagctg cgtgtcgaaa aagggtttag cccggatacg
    12601 ctcaagctgc tggaagcaaa aggtcagaaa gtggcgctga aagaggcgat gggcagtaca
    12661 caaagcatta tggttgggcc ggacggtgag ttgtacggcg tatccgaccc gcgctcggtg
    12721 gatgatttaa cggcggggta ctaaggttag cggccctctt cgtgggaaga gggctatttt
    12781 gtcagggcaa gccgaaggta gcctttttta tttcgtaatc ctgtagatat tcttccagcg
    12841 gctttgccgt tcccagagaa tgaatttcac cactgtcttt atcaataata aaaggtgcgt
    12901 taccagctaa gcgcgcggcc tcatctccag tttcgagaaa ttctcgtgct tcgaaacaga
    12961 aataccagcc ctggctaaag cgtccatgta gagtaatgac gaccgggaga tctgcatcat
    13021 caaggtaatg gttcgctttc gcgaatgcgt cgtgataagt aatcataatt ataataaata
    13081 ttattgttga gtgttatatt attattcctg tgtgatacat tgagcaaaga cgcgttcatc
    13141 ttcgtcaaag atattattaa atgctggttt tgaaaaaagt t
//
LOCUS       DAZFWL020000127        13041 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFWL020000127 DAZFWL020000000
VERSION     DAZFWL020000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.127"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..470
                     /locus_tag="ACHKNP_RS19425"
                     /old_locus_tag="pgaptmp_003885"
     CDS             <1..470
                     /locus_tag="ACHKNP_RS19425"
                     /old_locus_tag="pgaptmp_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGU4705969.1"
                     /translation="FSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFL
                     EMVICTGSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISIL
                     RGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(624..2273)
                     /locus_tag="ACHKNP_RS19430"
                     /old_locus_tag="pgaptmp_003886"
     CDS             complement(624..2273)
                     /locus_tag="ACHKNP_RS19430"
                     /old_locus_tag="pgaptmp_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HGU4705970.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2425..3774)
                     /gene="ghxP"
                     /locus_tag="ACHKNP_RS19435"
                     /old_locus_tag="pgaptmp_003887"
                     /gene_synonym="yjcD"
     CDS             complement(2425..3774)
                     /gene="ghxP"
                     /locus_tag="ACHKNP_RS19435"
                     /old_locus_tag="pgaptmp_003887"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HGU4705971.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4320..4784)
                     /gene="soxR"
                     /locus_tag="ACHKNP_RS19440"
                     /old_locus_tag="pgaptmp_003888"
     CDS             complement(4320..4784)
                     /gene="soxR"
                     /locus_tag="ACHKNP_RS19440"
                     /old_locus_tag="pgaptmp_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HGU4705972.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4870..5193
                     /gene="soxS"
                     /locus_tag="ACHKNP_RS19445"
                     /old_locus_tag="pgaptmp_003889"
     CDS             4870..5193
                     /gene="soxS"
                     /locus_tag="ACHKNP_RS19445"
                     /old_locus_tag="pgaptmp_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HGU4705973.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5196..6782)
                     /gene="pdeC"
                     /locus_tag="ACHKNP_RS19450"
                     /old_locus_tag="pgaptmp_003890"
     CDS             complement(5196..6782)
                     /gene="pdeC"
                     /locus_tag="ACHKNP_RS19450"
                     /old_locus_tag="pgaptmp_003890"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HGU4705974.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGCFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7212..7493
                     /locus_tag="ACHKNP_RS19455"
                     /old_locus_tag="pgaptmp_003891"
     CDS             7212..7493
                     /locus_tag="ACHKNP_RS19455"
                     /old_locus_tag="pgaptmp_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HGU4705975.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIELVMIEEMNQTPPQWPMILT"
     gene            complement(7592..8128)
                     /gene="ssb1"
                     /locus_tag="ACHKNP_RS19460"
                     /old_locus_tag="pgaptmp_003892"
     CDS             complement(7592..8128)
                     /gene="ssb1"
                     /locus_tag="ACHKNP_RS19460"
                     /old_locus_tag="pgaptmp_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HGU4705976.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8382..11204
                     /gene="uvrA"
                     /locus_tag="ACHKNP_RS19465"
                     /old_locus_tag="pgaptmp_003893"
     CDS             8382..11204
                     /gene="uvrA"
                     /locus_tag="ACHKNP_RS19465"
                     /old_locus_tag="pgaptmp_003893"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGU4705977.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11239..11595)
                     /locus_tag="ACHKNP_RS19470"
                     /old_locus_tag="pgaptmp_003894"
     CDS             complement(11239..11595)
                     /locus_tag="ACHKNP_RS19470"
                     /old_locus_tag="pgaptmp_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU4705978.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11599..12015)
                     /locus_tag="ACHKNP_RS19475"
                     /old_locus_tag="pgaptmp_003895"
     CDS             complement(11599..12015)
                     /locus_tag="ACHKNP_RS19475"
                     /old_locus_tag="pgaptmp_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HGU4705979.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12126..12839)
                     /gene="aphA"
                     /locus_tag="ACHKNP_RS19480"
                     /old_locus_tag="pgaptmp_003896"
     CDS             complement(12126..12839)
                     /gene="aphA"
                     /locus_tag="ACHKNP_RS19480"
                     /old_locus_tag="pgaptmp_003896"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HGU4705980.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKLGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLSQAGAFGEEVIVNSEY"
BASE COUNT         3373 a         3425 c         3196 g         3047 t
ORIGIN      
        1 gtttctctaa actcgattta attaactttg aacatgctga tctgtctcgc gtcaatctga
       61 acaaagcaat cctccagaat ataaacttaa ttgatagcaa actcttttgt acgtggctaa
      121 caaatacatt cctcgaaatg gttatatgta ccggctctaa tatggctaat gttaatttta
      181 ataatgccaa tttaagcaac tgccatttca actgttctat tttaacaaaa gcctgtatgt
      241 ttaatacccg tctctatcgg gttaattttg atgaggctag cgtccaggga atgggcattt
      301 ctattctccg tggggaggaa aatatcccca ttgatagtga taccctggta acactacaga
      361 aattctttga agaagattgt acctctcata ctggcatgtc acaaactgag gataatatta
      421 atgcagtcgc tatgaagatt actgcagata ttatgcaaca cgcagattga ggttaagtcg
      481 aagaacagcc aatgaattga ctttacaaga gagataactt atttgtttta gaatcatgaa
      541 tgttagcccg agccaacctg ttaaaggtgc aaaagattgc acctttttca gattacctcg
      601 ggcctggtta cgtcgctttc ggcttactga ttttcctcaa ttagcaacgc ttcaagcaaa
      661 tcgagatcgt gcagtaattt ttgcagcgtt tcgttgctga tctcccgcgt ggcgcgcagg
      721 tggtacagtt cagcacgttc agaacgcaat gccgccagac ggaagcgacg ctcaaggttc
      781 tcttcctgca cggaactttc aacgtcgtta cgtccatcgg cgcgacgacg caggttacca
      841 atgacgcgag aactgacctc cgtaagcagc tggttatcga tattctcttc ggtatccgcc
      901 gccagacgct cctccatttt ctggatcgcc acaatcgcca cttctgccgt tgccgctcgc
      961 gcaatacgtt cttctttcaa ttgttgcgaa tggtccgcga cttcaatgtg ttgtagcaga
     1021 ataggcaaca tcaccacgcc gacaaacagc gagaagagaa tgacgccagc cgccaggaac
     1081 accagctcat agcgcgccgg gaagacgtta ccatccggca agagcagcgg gatagagagc
     1141 acaccggcca gagtgatcgc cccacgcacc ccggcgaaag acgcgatcag gatttctcgt
     1201 gtggtccacg aaccaaactc catcggcttc ttcttcagga agcggttgct gaactttttc
     1261 atcgtccaca gccagccgaa acggaccagc atcagcgccg catatatcag aataatatcg
     1321 gtaaacagca tccagatttc gacgttaggg tcgatttctg ccgccatcag cgacgtctcc
     1381 agaatacccg gcagctgcag acctaacagc aggaacacca tgccgttaaa gacaaattcc
     1441 agcatcgccc aggtgctgtt tgcgcgcagg cgcattgcca gcggcgcacg gcgcatcaca
     1501 ccggagcggg tgatggtcat cccggcggca accgcagcga ggatgccgga aacgccaata
     1561 tgttcggcaa tcagataaga agcgaatggc agcaagaaca gcagaacgat ctgcgtcgcg
     1621 ggttcatcac cgccccagcg gctgaggaat cgcagcgagc gaccatacag ccagctcacc
     1681 acaaagccgg cgagaatacc gccaatggct actttcataa attcgaccgt cgcaccgcca
     1741 acggtgaaga tcatcgtccc catcgccact gccacggcaa acttcaacga caccaggccg
     1801 gaggcgtcat tcatcaacgc ttcgccctgc aaaataccca tgattttttt cgggatgcgc
     1861 ccttcgccaa caatcccgga gagcgccaca gcatcggtcg gagaaagcac tgccgccagc
     1921 gcaaaggcgg ggatcagcgg aatgcccggc accacccagt aaataaggaa gccaatgccg
     1981 accacggtga ccaccaccag cgccagcgcg aggccaaaaa tctctcgacc atgttcgaga
     2041 aattcacggg tcggcgtttt ccagccatca gcgaacagca acggcgggat aaacaagact
     2101 aaaaagagtt caggatcaaa ctccacatgc aaaccaaacg tcggccacgc cagtagcgca
     2161 ccgatggcga tttgcataag cggaagcggg atctgaaagg gcatgacacg agtgaccacc
     2221 ccggacagcg agaccacgag ggtcattatc agtatggtga agaagatttc catgcgttcc
     2281 ctgtttgcga tgttttttta tgatctgaga aagacgttat gcctgaattc tcattttttc
     2341 agaatatcgc aaaagctcac acaatgtgcg gagaaaataa aaacgggcag ccattgccac
     2401 ccgttttcag aatccgatga aagattagat agcccagcca cccgcataga aggtcaccag
     2461 cgccacggcg ataacgaccg taccgatgtt caacttgcgc cattcgccgg aaaccagacg
     2521 accaatcacc agagtcgcga agccgatcat gatgcctgtt acgatgttac aggtcagcac
     2581 gatgaatacc gccgtaacca gacccgccat cgcatcaaca aaatcagcaa agtcgatttt
     2641 cgccacgttg ctcagcatca gcaggccaac gtacatcagc gccggagccg ttgcataccc
     2701 cggaacgagg taagagagcg gagagaggaa cagaatcagc aggaacagca cgccaacggt
     2761 gatagccgtc aaaccggttt taccgcccgc cgccgtaccc gccgcagact cgatgtatac
     2821 cgctgccgga gcagcaccca ccaggccaga gaaaacgctg ctcatggagt cagtggtcag
     2881 tgctttccca ccgtcgatga tctgcccatc tttatccagc aggttcgcct ggccggcgac
     2941 ggcacggata gttccggtgg catcaaatac tgccgtcatc accagcgcca gaacgcttgg
     3001 caggactaca ggattcagcg cgcccataat gtccaggctg ccaatcagtg aattgccgtt
     3061 ttcatcgctc aatgaaggca tggcgaaaac gccggagaaa tggacgttag gatcgaagat
     3121 caaaccgaca attgagatac cgataatggt cagcagaatg ccaccaggga ctttcagttt
     3181 ttccaggccg atgatcaccg ccagacctac tagtgacata atcaccggga aggtcgcgaa
     3241 atcacccagc gcaacgggca gaccatcaag cgggttttta atcaccagac cgacaccatt
     3301 agcggcaatg agcagcagga acagaccgat accgatcccc gtgccgtgcg ccacaccgtg
     3361 cggcaagttg cgcaaaatcc agctacggat acccgtggca gaaattaccg taaacagcac
     3421 ccccatcagg aacacggcac ccagcgcgac aggtacgcta atatgttgcc ccagcaccag
     3481 gctgaatgcg gtaaacgccg tcagggagat ggcgcaacca atcgccaacg gcagattagc
     3541 ccacagaccc atcacgatag aaccgagtcc ggcaaccaga caggttgcaa cgaaaactgc
     3601 cgcaggcggg aagcctgctt tacccaacat acctggaacg acgatgaccg agtagaccat
     3661 cgccagaaac gttgttaacc cggcaaccac ttcctgacgg acagtgcttc cacgttgtga
     3721 aattttaaac caggcgtcga gtgaaccgcc ggtacgcgct gatggcgtag acataagaaa
     3781 acatcccctg agagtttaat tttcgtcaag atgcgtggcg gacaatcccc tgccagcgaa
     3841 acgttatctt cctgctccag gtttgcgaca atttactgcg tcgcaaaaaa gcaaacgttt
     3901 agctccgcgc atacaaaacg ggtggtaaaa aaagcaaacg attatccggc gatcatagcg
     3961 gctttttcaa ccgaagtttg ctgctttttt ctcttttatt gtcttgacga tgaaaaacca
     4021 ggcaattgat gcgcaaacgt tatcgtcggt gcgcaagatt gcagcgttat cgagccgtat
     4081 accgttcagg attgcgataa aaaatcgtct gagatgacaa aaatattgcg gataaaaagt
     4141 atacttttac actggcgaag aaatgccggt gacatacaat taaagcatca acaccaaccg
     4201 gaacctccac cacgtgctcg aatggggtgt gttgacgtcg ggggaaaccc tcctgtgtac
     4261 cagcgggata gagagaaaga caaagaccgg aaaacaaact aaagcgccct tgtggcgctt
     4321 tagttttgtt catcttccag caagcgtgcg ccggtacctt cttctcctaa gcggtcgccc
     4381 gggttacgca acgggcaatc actgcgcgaa aggcagccac aaccaataca tccgtccagt
     4441 tcgtcacgca gcgctactaa ggtatgaatg cgccgatcca actcttctcg ccattgggac
     4501 gaaagctgtt tccactcttt cgcacttaac gtatgccctt cgggcaacac gccaaacgct
     4561 tcaccaatgg tcgccagcgg aatgccaata cgctgagcaa ttttgataat tgcaacatat
     4621 cgcaacacat cacgtttata tcgccgctga ttgccgctgt tacggatact ggtaatcaac
     4681 cctttacttt catagaaatg cagcgccgat accgccacac cgctgcgttt cgccacttcg
     4741 ccgggggtta gcagcgcttt aatgcggggt aatttctttt ccataaatcg ctttacctca
     4801 agttaacttg aggaattata ctccccaaca gatgaattaa cgaactgaac actgaaaaga
     4861 ggcagattta tgtcccatca gaaaattatt caggatctta tcgcatggat tgacgagcat
     4921 attgaccagc cgcttaacat tgatgtagtc gcaaaaaaat caggctattc aaagtggtac
     4981 ttgcaacgaa tgttccgcac ggtgacgcat cagacgcttg gcgattacat tcgccaacgt
     5041 cgcctgttac tggccgccgt tgagttgcgc accaccgagc gtccgatttt tgatatcgca
     5101 atggacctgg gttatgtctc gcagcagacc ttctcccgcg ttttccgtcg gcagtttgat
     5161 cgcactccca gcgattatcg ccaccgcctg tagttttatt gcccgcgcgt taactcccgc
     5221 gcgggtaatt gctccatcca ttgcataaac acctgcggcg gcatcgcctt cgcaaaaaaa
     5281 catccctggc aatagcgcac gccgcgtttg cgcagccagt taacctgctc ctcagtttcg
     5341 acgccttcag cgatcgtttt taaccccagg ctgtgcgcca gctcgatgat gtgttccgca
     5401 atcaaatgac tggttttgtg ggtggtcagc gtttcaacaa acgatttatc gattttcaaa
     5461 atatcgacat tcaatgattt aaggttatgc aagttagagt agccaatacc aaaatcatca
     5521 attgccactt cgtaaccagc ctggcggaaa gccagaataa tcggcgtcat tttgtcaaca
     5581 tcaagaaatg catgttcagt cacttcaaat ttaatttgct gcggacgcac cgcgtattgc
     5641 tctgttttct gattgattcg cgctatcaac cgtgacgtat gaaaatcgga ggccgacagg
     5701 ttaatagaaa catagcgatc ggcatgtgtt gccaagtacg cgcccagatc acggaagaca
     5761 ttatcaataa cataatcagt tatctgttct atcatcccct ccttttctgc cagcggaata
     5821 aactctgccg gattcattat ttgcccctgc tcaccaagcc aacgtaacaa cgcttcagcg
     5881 ccgatacatt tttctgtttt gatatcgatt attggctggt aataaagaca aagttgatgt
     5941 ttttcgaggg cgcgttgcaa tttacgtttg ggagataaat agttttgtcg aatacgtagc
     6001 cagagtaata gcagaacaag actccccaaa ataccggcgg gcaacgcaaa aataagatga
     6061 ttataaaaat gggttataag acgttgatat gaagtcgcaa caatggctgc aattgggcgt
     6121 tttgtcgaat aaactgtcgc atataaatag ccatttcttt gtacggttaa atccttcaaa
     6181 tgaatcagcg gagaaaacgt tgctgccgag gcctctttgc ttaacgagaa aaaggttttc
     6241 gtcaccgtat catacacacc ccattgcaat gtcggatcat cagacatcac ttcactccag
     6301 aagagagggt tgataaccgc cacataattt ccccgctgca tataggtcat tttatagcca
     6361 gagaaaaaag gcgtatcgcg gtaataatag atagaaacgt taggttcacg cttataatcg
     6421 gccggtgcaa tcgtatagcc gtttacaggc gctatcagcg atgagcataa aaaatggtta
     6481 tcacgggcat agatcaattc attaatataa agatagccac gaataatatt caacattcgc
     6541 ttttgatggg ctggagtgca aacttgcccc tgatagcgtt cagctgcatc gctcactaaa
     6601 tctgcctggc gaatgaccag ctcggattta tccagtgcca gttgagcaaa ggttcggagc
     6661 tgattattca cttctgattt tgcccaaagg aaggcaatcc atagcgaaag aatgatggga
     6721 aagagaacta aaaagataat gcccggcaac gccagtaatt ggtgtcgtgc acgatgactc
     6781 attgctatca atttcctgcc tgtttatacc cggccattgc aaggtgaaaa ccctgggtac
     6841 cgacgtcttt agataaatct tacggtataa atagcacttt tgcctgattt gaaattgata
     6901 cctttctaat cgtcacctgg atgaaaataa ttcattgata ttcaaaatta atttcctaca
     6961 ggaatgcaga ggcggcggga agatatgccc ttaatgccac tgaaaaacca cataatctgt
     7021 acagcaataa aagtcacggc ctaatatggc taacaaacaa tcaattgtga tatagttcac
     7081 aaaattaatg aaacaaacag agtgtttcat ttttgtgttc tgcaagcccc gcgatagcca
     7141 gagtgtacca cgcctcccgt gaacaacgcc gcgctgtcca gggcatcggc tcttttcaga
     7201 ggaattgttt gatggctacc ctcaccactg gcgtggttct tcttcgctgg caacttctta
     7261 gtgccgtaat gatgtttctg gccagcacac tcaacatccg ttttcgtcgg tctgattatg
     7321 tcgggcttgc ggtgatcagc agcggtctgg gcgttgtttc cgcctgttgg ttcgcaatgg
     7381 ggttgcttgg catcacaatg gcggacatca ccgctatctg gcacaacatc gagttggtga
     7441 tgatagaaga gatgaatcag acaccgccac aatggccaat gattttgacc tgatacgtag
     7501 aagcctccat aacggaggct tcttttttat ggctggcaga tgctttaatc atccacctta
     7561 aaacaatata acctattgtt ttaatgacaa atcagaacgg aatgtcatca tcaaagtcca
     7621 tcggcggctc gttagacggc gctgccggag cggactgctg cgggcgagac tgcgcgccgc
     7681 cgctgaactg attgccaccc tgcggctgct gtggctgacc ccaaccgccc tgcggctgac
     7741 caccaccgat attgccacct gccggagcgc caccaccctg acgaccaccc agcatctgca
     7801 tggtgccgcc aacgttcacc acgacttctg tggtgtagcg atcctgaccg gattgatcgg
     7861 tccatttacg ggtacgcagc tgaccttcga tataaacctg agaaccttta cgcagatatt
     7921 cgctcgccac ttctgccagt ttgccgaaca gcacaacgcg gtgccattca gtctgttctt
     7981 tcatctcgcc ggtcgcttta tcacgccagg attcggaagt agccagcgta atgttggcaa
     8041 ctgcgccacc atttggcatg tagcgtactt ccgggtcctg acccagatta ccaacgagaa
     8101 taaccttgtt tacgcctctg ctggccatgt tcgtgtctcc tgaaaaaaat cgttctgaat
     8161 aagtgtaaac gcgcgattgt accattacca atagcgcttt tactatgttg tgacctcggt
     8221 tccgggaaac aaacctggcc agacattgtt acacaacact ccgggtaatg cattccaata
     8281 ctgtatattc attcaggtca atttgtgtca taattaaccg tttgtgatcg ccggtagcac
     8341 catgccaccg ggcaaaaaag cgtttaatcc gggaaaggtg aatggataag atcgaagttc
     8401 ggggcgcccg cacccataat ctcaaaaaca tcaacctcgt tatcccccgc gacaagctca
     8461 ttgtcgtgac cgggctttcg ggttctggca aatcctcgct cgctttcgac accttatatg
     8521 ccgaagggca gcgccgttac gttgaatccc tttccgccta cgcgcggcag tttctgtcac
     8581 tgatggaaaa gccggacgtc gatcatattg aggggctttc tcctgccatc tcaattgagc
     8641 agaaatcgac gtctcataac ccgcgttcta cggtggggac aatcaccgaa atccacgact
     8701 atttgcgttt gttgttcgcc cgcgtcggcg aaccgcgctg tccggaccac gacgtcccgc
     8761 tggcggcgca aaccgtcagc cagatggtgg ataacgtgct gtcgcagccg gaaggcaagc
     8821 gtctgatgct gctcgcgcca atcattaaag agcgcaaagg cgaacacacc aaaacgctgg
     8881 agaacctggc aagccagggt tacatccgtg ctcgtattga tggcgaagtc tgcgatcttt
     8941 ccgatccgcc gaaactggaa ctgcaaaaga aacataccat tgaagtggtg gttgatcgct
     9001 tcaaggtgcg tgacgatctt acccaacgtc ttgcggagtc gtttgaaacc gcgctggagc
     9061 tttccggtgg tacagcggta gtggcggata tggacgaccc gaaagcggaa gagctgctgt
     9121 tctccgccaa cttcgcctgc ccaatttgcg gctacagtat gcgtgaactg gagccgcgac
     9181 tgttttcgtt taacaacccg gcaggtgcct gcccgacctg tgacggcctt ggcgtacagc
     9241 aatatttcga tcctgaccgc gtgatccaaa accccgagct gtcactggct ggcggtgcga
     9301 tccgtggctg ggatcgccgc aacttctatt acttccagat gctgaaatcg ctggcagatc
     9361 actataagtt cgacgtcgaa gcgccgtggg gcagcctgag cgcgaacgtg cataaagtgg
     9421 tgttgtacgg ttctggcaaa gaaaacattg aattcaaata catgaacgat cgtggcgata
     9481 cctccatccg tcgtcatccg ttcgaaggcg tgctgcacaa tatggagcgc cgttataaag
     9541 agacagaatc cagtgcggta cgtgaagaat tagccaagtt tatcagcaat cgcccatgcg
     9601 ccagctgcga agggacgcgt ctgcgtcggg aagcgcgcca cgtttatgtc gagaatacgc
     9661 cgctgcctgc tatctccgac atgagcatcg gtcatgcgat ggaattcttc aacaatctca
     9721 aactcgctgg tcaacgggcg aagattgcgg aaaaaatcct taaagagatc ggcgatcgtt
     9781 tgaaattcct cgttaacgtc ggcctgaatt acctgacgct ttcccgctcg gcagaaacgc
     9841 tttccggcgg tgaagcccag cgtatccgtc tggcgagcca gattggtgcg ggcctggttg
     9901 gcgttatgta cgtgctggac gagccatcta tcggcctgca ccagcgcgat aacgagcgcc
     9961 tgttgggtac gcttatccat ctgcgcgatc tcggtaatac cgtgattgtg gtggagcacg
    10021 acgaagacgc aattcgcgcc gctgaccatg tgatcgacat tggcccgggc gcaggtgttc
    10081 acggcggtga agtggtcgca gaaggtccgc tggaagcgat tatggcggtg ccggagtcgt
    10141 tgaccgggca gtacatgagc ggcaaacgca agattgaagt gccgaagaaa cgcgttccgg
    10201 cgaatccgga aaaagtgctg aagctgacag gcgcacgcgg caacaacctg aaggacgtga
    10261 cgctgacgct gccggtgggt ctgtttacct gcatcaccgg ggtttcaggt tccggtaaat
    10321 cgacgctgat taacgacaca ctgttcccga ttgcccaacg ccagttgaat ggggcgacca
    10381 tcgccgaacc agcaccgtat cgcgatattc aggggctgga gcatttcgat aaagtgatcg
    10441 atatcgacca aagcccaatt ggtcgtactc cacgttctaa cccggcgacc tataccggcg
    10501 tgtttacgcc tgtgcgcgaa ctgtttgcgg gcgtaccgga atcccgtgcg cgtggttata
    10561 cgccaggacg tttcagcttt aacgtccgtg gcgggcgctg cgaagcctgt cagggcgacg
    10621 gtgtgatcaa agtagagatg cacttcctgc cggatatcta cgtgccgtgc gatcagtgta
    10681 aaggtaaacg ctataaccgt gaaacgctgg aaattaagta caaaggcaaa accatccacg
    10741 aagtgctgga tatgaccatc gaagaggcgc gtgagttctt tgatgccgta cctgcactgg
    10801 cgcgtaagct gcaaacgttg atggacgttg gcctgacgta cattcgcctg gggcagtccg
    10861 caaccacact ttctggtggt gaagcccagc gcgtgaagct ggcgcgtgag ctgtcaaaac
    10921 gcggcaccgg gcagacgctg tatattctcg acgagccgac caccggtctg cacttcgccg
    10981 atattcagca actgctcgac gtactgcata aactgcgcga tcagggcaac accattgtgg
    11041 tgattgagca caatctcgac gtgatcaaaa ccgctgactg gattgtcgac ctgggaccag
    11101 aaggcggcag tggcggcggc gaaatcctcg tctccggtac gccagaaacc gtcgcggagt
    11161 gcgaagcttc gcatacggca cgcttcctca agccgatgct gtaatcgtta aggccgcttt
    11221 ctgagcggcc ttttcctttc agagttgtac cagcaattta cgcttttctt ccggtagtaa
    11281 attcactgcc tgctgataag acgcatccac cagataatag atttgcgaat ccggcagcga
    11341 accgtcgaga tacacggtgc tccagtgcgc tttattcaga tggcggctag gacgcacatc
    11401 gctgtgctgc tgacgtagca gctccgccag ctccgggctg gttttcagcg aaacagctgg
    11461 gcgattttct acttctttca ccatcgcaaa cagtacatct tccactttga tctgcgtcgc
    11521 tttccagtcg ttatgcacgc tctgttctgc gcctggtttt gccatgcaat attgtagcaa
    11581 ctccgaaatg gtcatttttt actccccttg tagtgtcgcg atgatgcgac gcgatccgcc
    11641 gtggatgcga tgttccccca gccagatgcc ttgccaggtg ccggtctgaa tacgcccttt
    11701 atgtaccggc aatacaagcg atgttcccag cattgaggat ttgatatgag aaggcatatc
    11761 gtctgctccc tcatagtcat gctcataatt tccgttgtcg ggaacggtgc ggaggaaaaa
    11821 acgctccatg tcgtggcgta cggtgggatc gcagttctca ttaagtgtca gagaggcgga
    11881 ggtatgttgc agcaacagat gcagtaagcc gatgttaacg cgcggcatat cagccagctg
    11941 attcagaatt tcatccgtta ccagatgaaa cccacgagat ttggcgctaa gcgtgagcgt
    12001 cttttgatac cacatgtgct gctccttgat aaaactctct taatcagttt gcagcaagac
    12061 agcgaaagga taaaggtgtg attaaaaaaa cagcattcgg gagagcgtca cgctctcccg
    12121 ctctgtcagt attctgaatt gacgatcacc tcttcaccaa acgcacccgc ttgtgacaag
    12181 ggtttgtagg tagagttgga ggcgcgcaga atgcggatac cacgagcgcc gacatcgcgt
    12241 gcggcggtaa tatcattatc agaatcgcca taaaaaattc ggatattttt atcctgcagc
    12301 cattgcgatt ttgtattttg ccctagttta tcgcccgcaa agatcaccgg attcatgttg
    12361 gtggcaggaa tatgaaaatt atccgccagc gtttttgaaa ccgtttctgt tttcgtcgga
    12421 ctacgaccag tcacaaagaa gatcgcgtca ccgcggcgta catgcatatc aatcagctgg
    12481 cgagcgacct cttttggaat gctgaattca tcccagccat tgttcatttt ttcccagaac
    12541 acaggatttt tcagataatc ttcgctttct ggcgagaagg tttttttgcc gcgccagaag
    12601 cccggactgg aaaaaagtac cgtgtcatcg atatcaaacc ccaccgccat tggcggacgc
    12661 cctgcgaggc tattttcaat ttgtgcgacc gaaacccaat gaatgggtgc ctgttcagca
    12721 agcctggcaa cgttagtccc agggttaagc ggtgaaggag atgaggccag ggcaacagcg
    12781 gaactgttta gagcgaacaa taagcaaacg gcactgattg cctgtgtgat cttgcgcata
    12841 tttttcccta aatagtcagt atgttgaaac tttttattgt gagatttgtt gcaaaaacca
    12901 tctgaccata acgccagctg ccggggaaag gaaggattat ctgcggtttt tgtgatgaaa
    12961 agcaggatga ttatatttaa cccagtaaat acgttcctgt ctttttttgt ataaaaaatg
    13021 tttattactc gatgtaagga t
//
LOCUS       DAZFWL020000128        13038 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFWL020000128 DAZFWL020000000
VERSION     DAZFWL020000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.128"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            335..916
                     /gene="mdaB"
                     /locus_tag="ACHKNP_RS19485"
                     /old_locus_tag="pgaptmp_003897"
     CDS             335..916
                     /gene="mdaB"
                     /locus_tag="ACHKNP_RS19485"
                     /old_locus_tag="pgaptmp_003897"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HGU4705981.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            947..1261
                     /locus_tag="ACHKNP_RS19490"
                     /old_locus_tag="pgaptmp_003898"
     CDS             947..1261
                     /locus_tag="ACHKNP_RS19490"
                     /old_locus_tag="pgaptmp_003898"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HGU4705982.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1309..3201)
                     /gene="parE"
                     /locus_tag="ACHKNP_RS19495"
                     /old_locus_tag="pgaptmp_003899"
     CDS             complement(1309..3201)
                     /gene="parE"
                     /locus_tag="ACHKNP_RS19495"
                     /old_locus_tag="pgaptmp_003899"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGU4705983.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3230..3811)
                     /gene="yqiA"
                     /locus_tag="ACHKNP_RS19500"
                     /old_locus_tag="pgaptmp_003900"
     CDS             complement(3230..3811)
                     /gene="yqiA"
                     /locus_tag="ACHKNP_RS19500"
                     /old_locus_tag="pgaptmp_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HGU4705984.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3811..4638)
                     /gene="cpdA"
                     /locus_tag="ACHKNP_RS19505"
                     /old_locus_tag="pgaptmp_003901"
     CDS             complement(3811..4638)
                     /gene="cpdA"
                     /locus_tag="ACHKNP_RS19505"
                     /old_locus_tag="pgaptmp_003901"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HGU4705985.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYRHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4663..5085)
                     /locus_tag="ACHKNP_RS19510"
                     /old_locus_tag="pgaptmp_003902"
     CDS             complement(4663..5085)
                     /locus_tag="ACHKNP_RS19510"
                     /old_locus_tag="pgaptmp_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HGU4705986.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5086..5715)
                     /gene="nudF"
                     /locus_tag="ACHKNP_RS19515"
                     /old_locus_tag="pgaptmp_003903"
     CDS             complement(5086..5715)
                     /gene="nudF"
                     /locus_tag="ACHKNP_RS19515"
                     /old_locus_tag="pgaptmp_003903"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HGU4705987.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5920..7401
                     /gene="tolC"
                     /locus_tag="ACHKNP_RS19520"
                     /old_locus_tag="pgaptmp_003904"
     CDS             5920..7401
                     /gene="tolC"
                     /locus_tag="ACHKNP_RS19520"
                     /old_locus_tag="pgaptmp_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HGU4705988.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7549..8220
                     /locus_tag="ACHKNP_RS19525"
                     /old_locus_tag="pgaptmp_003905"
     CDS             7549..8220
                     /locus_tag="ACHKNP_RS19525"
                     /old_locus_tag="pgaptmp_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HGU4705989.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8226..9386
                     /locus_tag="ACHKNP_RS19530"
                     /old_locus_tag="pgaptmp_003906"
     CDS             8226..9386
                     /locus_tag="ACHKNP_RS19530"
                     /old_locus_tag="pgaptmp_003906"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU4705990.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9424..10212)
                     /gene="ygiD"
                     /locus_tag="ACHKNP_RS19535"
                     /old_locus_tag="pgaptmp_003907"
     CDS             complement(9424..10212)
                     /gene="ygiD"
                     /locus_tag="ACHKNP_RS19535"
                     /old_locus_tag="pgaptmp_003907"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HGU4705991.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10355..11128
                     /gene="zupT"
                     /locus_tag="ACHKNP_RS19540"
                     /old_locus_tag="pgaptmp_003908"
     CDS             10355..11128
                     /gene="zupT"
                     /locus_tag="ACHKNP_RS19540"
                     /old_locus_tag="pgaptmp_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HGU4705992.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11186..11356)
                     /gene="yqiD"
                     /locus_tag="ACHKNP_RS19545"
                     /old_locus_tag="pgaptmp_003909"
     CDS             complement(11186..11356)
                     /gene="yqiD"
                     /locus_tag="ACHKNP_RS19545"
                     /old_locus_tag="pgaptmp_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HGU4705993.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11617..12270)
                     /gene="ribB"
                     /locus_tag="ACHKNP_RS19550"
                     /old_locus_tag="pgaptmp_003910"
     CDS             complement(11617..12270)
                     /gene="ribB"
                     /locus_tag="ACHKNP_RS19550"
                     /old_locus_tag="pgaptmp_003910"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HGU4705994.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12374..12522)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12644..12934
                     /gene="ubiK"
                     /locus_tag="ACHKNP_RS19555"
                     /old_locus_tag="pgaptmp_003911"
     CDS             12644..12934
                     /gene="ubiK"
                     /locus_tag="ACHKNP_RS19555"
                     /old_locus_tag="pgaptmp_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HGU4705995.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3303 a         3479 c         3174 g         3082 t
ORIGIN      
        1 ttttatgctt taacatatat cagtacgctc ataaaaatcc atttataatt atcaacatga
       61 cacgttgata ttttcatctt ttacttattc aaagccatat tttcaatccc aaaaaaaacc
      121 gaaccaaacg tgtaaattat tccggtgcga gtaagaatta tgaggaatgg ctatcagtat
      181 tgtcattttc agaaaatatt tatcctgcat cggtgagtca gagcaagatc agacttttgc
      241 taaattcgca aaagactttg cacattttgc taatttcacc gtaccgctct gtgacgtact
      301 atagtcggca aacgtctcac cttgaggtta aaaaatgagc aacatcctga ttatcaacgg
      361 cgcgaaaaaa ttcgcccact ccaatggtca actgaacgac accctgaccg aagtcgcgga
      421 tggcacactg cgcgacctcg ggcatgatgt ccgcatcgtt cgcgccgaca gcgactacga
      481 tgtcaaagcg gaagtacaaa actttctctg ggctgatgtg gtgatctggc agatgccagg
      541 ctggtggatg ggcgcgccgt ggacagtgaa aaaatatatt gatgatgtat tcaccgaagg
      601 tcatggaacg ctgtatgcca gcgatggccg tacccgcaaa gatccgtcaa aaaaatacgg
      661 ttccggcggc ctggtacagg gcaaaaaata tatgctttct ctgacctgga acgcaccaat
      721 ggaagccttc accgaaaaag atcaattctt ccacggcgtt ggcgtcgacg gtgtgtatct
      781 gccgttccat aaagcaaacc aattccttgg catggaaccg ctgccgacat ttatcgctaa
      841 tgacgtgata aaaatgcctg atgttccccg ctatactgaa gaatatcgca agcatcttgt
      901 ggaaattttt ggttaactag agctcaggct ttagaaggag ttaaccatgc ttaccgtaat
      961 cgcagaaatc cgtactcgtc ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc
     1021 taaaatcgtt ccaaccgtac tgaaagaaga aggttgccac ggctatgcgc caatggtgga
     1081 ttgcgctgct ggcgtgagtt tccagtctat ggcaccggat tctatcgtga tgattgagca
     1141 gtgggaaagc atcgcgcatc ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag
     1201 cgaagccgta aaaggcgacg tgctggagat gaatatccgc atcctgcaac ctgggattta
     1261 aacctgcctt gtttgcccgg ccatcctgac cgggcaatgt tctttccttt aaacctcaat
     1321 ctccgccatg tcgcctttct cttgcaacca gttgcggcga tcttccgagc gtttcttcgc
     1381 cagcagcata tccatcatcg cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg
     1441 caccagacgg cgagtgttcg gatcaagcgt ggtttcacgc aattgcatcg ggttcatttc
     1501 ccccagacct ttaaaacgct ggacgttcgg cttgcctttc ttgcgtttta attgctcaag
     1561 tacgccctct ttctcttctt ccgtcagcgc gtaataaacc tctttcccga gatcaatacg
     1621 gtagagcggt ggcagtgcga cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt
     1681 tacgaacaaa gcgcagagca gcgtggcaat gtgcagacca tcagagtccg catccgcgag
     1741 gatacagatt ttgccataac gaagctggct cagatcgtcg ctgtcaggat cgataccgat
     1801 cgctaccgaa atatcatgca cttcctgcga ggccagcact tcgtcggaag aaacttccca
     1861 ggtgttaagg atcttacctt tcagtggcat gatcgcctga tattcgcgat cgcgcgcctg
     1921 cttggcagat ccgcctgcgg agtcaccttc cacaaggaac agttcggtac ggttaagatc
     1981 ctgcgcggta cagtcagcca gtttgccagg cagtgctggg ccgctggtca gctttttgcg
     2041 caccactttt ttagccgcac gcatacggcg ctgggcgctg gaaatcgcca tctccgccag
     2101 cagctccgcc gcctgaacgt tctggttcag ccacaggata aaggcatctt tcaccacgcc
     2161 tgaaacgaat gccgcgcatt gacgcgaaga gagacgctct ttggtctgcc cggcaaactg
     2221 cggatcctgc attttcaccg acagaacata ggcgcagcga tcccagatat cttccgccga
     2281 cagcttcacg ccgcgcggca gaatgttgcg gtattcacag aactcacgca tcgcgtccaa
     2341 caggccctga cgcagaccat taacatgggt accgccctgc atcgttggga taaggttgac
     2401 gtagctttcg gtcagcagtt caccgccttc cggcagccac agtagcgccc agtccacagc
     2461 ttcagtatcg ccagcgaaat taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt
     2521 taccgcttcc gccaggtaat cattcagacc gtcctgatag caccagcgct gttcggtgtt
     2581 gttgatctca tctttaaagg taatctcaac gccggggcac agtactgctt tggccttcag
     2641 cacatgcgtc aggcgtgaaa cagaaaaacg cgggctgtca aagaaggttt catccggcca
     2701 gaagtgcacg ctggtaccgg tattgcgttt accgcaagtg ccgacaacct gtaaatcctg
     2761 caccttttcg ccattttcaa aggcgatgtt ataaacctga ccatcgcggc gcacgttaac
     2821 ttctacgcgc ttcgacaggg cgttaaccac cgaaatcccc acgccatgca ggccgccaga
     2881 gaactggtaa tttttgttag agaatttacc gcctgcatgc agacggcaaa gaatcagttc
     2941 aaccgccggt acaccctctt ccgggtgaat atccaccggc atcccgcgcc catcgtcaat
     3001 aacttctaac gactggtcag catgtaagat tacatccacg cgttttgcgt gacccgccag
     3061 tgcttcatcg acactgttat caataacttc ttgccccaaa tggttagggc gagtggtatc
     3121 ggtatacatc cccggacggc ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc
     3181 agcgttataa gtttgcgtca tggtttaagt tagcaattcg agttgatcgt cagagatggt
     3241 gcagaccaag aaaatcgacg atcgggttga aataatcttc gaagcccgtg aatgcgtggt
     3301 tgccgccttc tatgacagtc tggcggcagg aagcgtagta cgccaccgcc tggcggtaat
     3361 ccagcacttc atctcccgtc tgttgcagca gccagatcaa atccggcgct tccagcgggt
     3421 caatctgcat gactttgaga tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg
     3481 tgtaggggtt ctcgttctga ccgagatagt ccgtcagcag ttcaaacggg cgcaccgccg
     3541 ggtttaccac cactgcgggc agcataaaac attgtgacaa ccaggtggcg taatatcccc
     3601 ccagtgacga accgacaata cccagcgaat cgccgccatg ttccaggaca atggattcca
     3661 gcagctctgc cgcgtcggaa ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag
     3721 ggtgatgttc cgccagccag ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt
     3781 tgaaaccgtg taaataaaga agcgtagaca tcagtagcct tctgaagcgg tatcaggttg
     3841 gaaacgtgtg tccgccaggc gatgcacctc ggtggtcagc gtgccatcag catgtaactc
     3901 gagagtacgc cagccgggcg cgatggtgtc cagcgtaaag ttggaacagt gcggcttaaa
     3961 ctgcacacag gtcgacggcg ttgccagcag gcggcgaccg ttccagtcga gatccagctc
     4021 ctgatgaata tgaccgcaca gcaagtattt gacgtgcgga aacttcgcca gcacggtatc
     4081 cagttcgccc gcgttacgca ggctgtgttg atcaagccaa ctacaacccg caggtagcgg
     4141 atgatgatgc agcagcagca acgtatggcg ttctggcgca tcggccagtt tacgttccag
     4201 ccactcaagc tgaaactcgc tcagctcacc gtgcggcacg ccaaacacct ggctatccag
     4261 caacaggatt tgccattgct caccaataaa cacccgcttc gccggggaga tacccgcatc
     4321 ctgtaacgcg ctgtacatcg cgggctggaa atcgtggttg ccaggcagcc agacgcaggg
     4381 cgcacgaaaa cttgcgatgc cttcagcgaa atgccgatag gccgcagagg attgatcctg
     4441 cgctaaatca cctgtcgcga caatcaggtc gaattcgtgc tggtgtggac gaatcgcctc
     4501 cagcaccgcc tggtagcttt cccaggtatt tacccctaac agggcttcgt gcttttgtgc
     4561 aaacaggtga gtatcggtaa tttgtaaaat cctgactctg gcctcaccag ccagaggaag
     4621 ggttaacagg ctttccaaat ggtgtcctta ggtttcacga cgctagtaaa ccggaatcgc
     4681 catcgctcca tgtgctaaac agtatcgcaa ccagtccgct aaaaactggt taatttgatg
     4741 cttttcgtcg cgttgatgca actttttatt aggataatca taccgcgctt tgaagcgaaa
     4801 aatctgctgg cttgaacaca cttcagccac catcgcgtca tgatacagac gcaccgtcat
     4861 tgacggaagg ctccagtaac tgatcgcggg cgcagtctgt tctattgtca ccagggtagt
     4921 gtatcgggtc gattccacaa tcgtcagccg atattgtgcg tttgccacct gatagcttac
     4981 agtttcgccg ggtgcgtcat tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc
     5041 gcacaggcgc atcatttcag gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt
     5101 ttttaacgct tgatgatgca gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc
     5161 gattttcccc tcttctaccc actggtatgc ctgttcccgg cttaccacat gaacgcgaat
     5221 atcttcgttt tcatcagcca gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc
     5281 caccataatt gacgaacgct cactggtgcc ccccgggctt gccaggaaac ttaacaccgg
     5341 tttggtccgt ttgactatca gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc
     5401 cacactttca ccctcttcta tcatcccggc aaccatctcc agtagccagg gggtttcgct
     5461 ggtgtcgtac gcggcaatcc gaatctgctc aatcagcaca acttcatcac gcactgggtc
     5521 aaagggtagc aagactgcgg cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact
     5581 catttgcccg ttgaatagac gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg
     5641 ataaagtgtt tctcgtgcaa taatttctac atcgtttttg ccaaatgtaa cgggcaggtt
     5701 gtctggctta agcattgtta atgtcctggc actaatagtg aattaaatgt gaatttcagc
     5761 gacgtttgac tgccgtttga gcagtcatgt gttaaattga ggcacattaa cgccctatgg
     5821 cacgtaacgc caaccttttg cggtagcgga ttctgctaga atccgcaata attttacagt
     5881 ttgatcgcgc taaatactgc ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt
     5941 cttatcggcc tgagcctttc tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa
     6001 gtttatcagc aagcacgcct tagtaacccg gaattgcgta agtctgccgc cgatcgtgat
     6061 gctgcctttg aaaaaattaa tgaagcgcgc agtccattac tgccacagct aggtttaggt
     6121 gcagattaca cctatagcaa cggctaccgc gacgcgaacg gcatcaactc taacgcgacc
     6181 agtgcgtccc tgcagttaac tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg
     6241 ctgcaggaaa aagcagcagg gattcaggac gtcacatatc agaccgatca gcaaaccttg
     6301 atcctcaaca ccgcgaccgc ttatttcaac gtgttgaatg ctattgacgt tctttcctat
     6361 acacaggcac aaaaagaagc gatctaccgt caattagatc aaaccaccca acgttttaac
     6421 gtgggcctgg tagcgataac cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg
     6481 gcgaacgaag tgaccgcacg caataacctt gataacgcgg tagagcagct gcgccagatc
     6541 accggtaact actatccaga actggcagcg ctgaatgtcg aaaactttaa aaccgacaaa
     6601 ccacagccgg ttaacgcgct gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag
     6661 gcacgcttga gccaggacct ggcgcgcgag caaattcgcc aggcgcagga tggtcactta
     6721 ccgacgctgg atttaacggc ttctaccggg atttctgaca cctcttatag cggttcgaaa
     6781 acccgtggtg ccgctggtac ccagtatgac gacagcaata tgggccagaa caaagtgggc
     6841 ctgagcttct cgctgccgat ttatcagggc ggaatggtta actcgcaggt gaaacaggca
     6901 cagtacaact ttgtcggtgc cagcgagcaa ctggaaagcg cgcatcgtag cgtcgtgcaa
     6961 accgtacgtt cctccttcaa caacattaat gcatctatca gtagcattaa cgcctacaaa
     7021 caagccgtag tttccgctca aagctcatta gacgcgatgg aagcgggcta ctcggtcggt
     7081 acgcgtacca ttgttgatgt gttggatgca accaccacgc tgtacaacgc taagcaagag
     7141 ctggcaaatg cgcgttataa ctacctgatt aatcagctga atattaagtc agccctgggt
     7201 acgttgaacg agcaggatct gctggcactg aacaatgcgc tgagcaaacc ggtttccact
     7261 aatccggaaa acgttgcccc gcaaacgccg gaacagaatg ctattgctga tggttatgcg
     7321 cctgatagcc cggcacccgt cgttcagcaa acatccgcac gcactaccac cagtaacggt
     7381 cataaccctt tccgtaactg atgacgacga cggggcttcg gccccgtctg aacgtaaggc
     7441 aacgtaaaga tacgggttat ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag
     7501 ccatcctcta ttctgatggg tatttaccac tggtcccgga agacaaaaat gaaacggaca
     7561 aaatccatac gccacgcatc gttccgcaaa aactggagcg cgcgccatct gacaccagtc
     7621 gctctcgcgg ttgccactgt ttttatgctg gctggctgtg aaaagagtga tgaaacagtg
     7681 tctctctatc aaaatgctga cgactgttca gctgcaaacc caggcaaaag cgccgaatgt
     7741 accaccgcgt acaacaatgc gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc
     7801 cgtgaagact gtgttgctga atttggtgaa ggtcagtgcc agcaggcacc agcccaggct
     7861 ggcatggcac cagaaaacca ggcgcaggcc cagcaatcca gcgggagttt ctggatgccg
     7921 ctgatggccg gttacatgat ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag
     7981 ccgctgttct cctcgaaaaa cccagccagc ccggcttacg gtaaatatac cgacgcgacg
     8041 ggtaaaaact atggcgcagc ccagccaggc cgcaccatga ccgtaccgaa gacagccatg
     8101 gcaccaaaac cggcgaccac cactaccgtt acccgtggcg gttttggtga atctgttgcc
     8161 aaacaaagca ctatgcagcg tagcgcaact ggcacctctt ctcgttcaat gggtggctga
     8221 taccgatgga aagagtcagt attaccgagc gcccggactg gcgtgagaaa gcccacgaat
     8281 acggtttcaa ttttcacacc atgtacggcg agccgtactg gtgtgaagat gcttactaca
     8341 agttgaccct cgcccaggtt gaaaagctgg aagaagtcac cgccgaactg caccagatgt
     8401 gcctgaaagt ggtggaaaaa gtgatcgcca gcgatgagct gatgaccaaa ttccgcattc
     8461 caaaacacac ctggagtttt gtgcgccagt catggctgac gcaccagcca tcgctttatt
     8521 cgcgtcttga tctggcgtgg gatggcactg gcgaacctaa acttctggaa aataacgccg
     8581 atacgccaac gtcactatac gaggcggcgt tctttcagtg gatctggctg gaagatcagc
     8641 ttaacgccgg taacttgccg gagggcagcg accagtttaa cagtctgcaa gaaaaactga
     8701 tcgatcgctt cgttgagctg cgtgaacagt atggcttcca gttgctgcat ctcacctgct
     8761 gtcgcgacac ggtggaagat cgcggaacca ttcagtattt gcaggactgc gcaacggaag
     8821 ctgaaattgc tactgagttc ctctacatcg atgatatcgg gttaggtgaa aaaggtcagt
     8881 tcacggattt acaggatcag gtaatttcca acctgttcaa gctgtatccg tgggaattta
     8941 tgttgcgtga gatgttttcc accaagctgg aggatgcagg cgtacgctgg ctggaaccgg
     9001 cgtggaagag cattatctcc aacaaggcac ttctaccgct actgtgggag atgttcccga
     9061 atcacccgaa cctgctgccc gcttattttg cggaagatga tcatccgcaa atggaaaaat
     9121 atgtggttaa accgatcttc tcccgtgaag gcgcaaacgt gtcgatcatt gaaaacggca
     9181 aaaccattga agcagcggaa ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc
     9241 acccgttacc gaaattcggc gacagctata tgctgattgg tagctggctg gtgaacgatc
     9301 aacccgccgg aattggcatt cgtgaagacc gtgcattgat cacccaggat atgtctcggt
     9361 tttatccaca tatttttgtt gagtaatcaa tgatgccgga tggcattcgc catccggtaa
     9421 ttgttaacct atctgcaccg acagcatact caggctgccc atttctatac catcgaccgg
     9481 aatggtaatt ggctcctgcc catcccacgc acctaagaca tacaacaacg gcagatagtg
     9541 ctccggcgtt gggttcgata acgcgccacc ttcatggtcg aggtaattca ccagaggatg
     9601 ctgctctacc gccccttgcc acgtcagatt cgctttcaca tactcattaa acgacatcgc
     9661 ccaaggatat ggtgaactat caccgtgcca cttcactgta cgcaggttat gcaccacgtt
     9721 gccactggcg accaacatta ttccttcatc tcgcagcgct gccagtttgc gccccatttc
     9781 gaaatgccag gcggcaggtt tgctactgtc gatactcaac tgcaccatcg ggatatcggc
     9841 gtcaggatac atcttaatca gcacgcccca ggaaccgtgg tcaaagcccc aggcttcttt
     9901 atccagcgtc accgggaccg gtgctaacag ctcaaccaga tgctgtgcca gcacaggcga
     9961 acccggagca ggataatgcg tatcgtacag cgcctgcggg aagccaccaa agtcatgaat
    10021 cgtgggcggc gtctccatcg cggtcactcc tgttccacgg gtaaaccagt gagccgaaac
    10081 caccacaatc gcttgcgggc gtggcaatgt catccccaac ttctgccagc tgcgggtata
    10141 caaattatct tccagcacgt tcatcggact accgtgacct aaaaacaatg ctggcatacg
    10201 tgttgaagac atgatgatat ccttaactaa aggtgtcatt ttgatatcct cacaatacgc
    10261 ttgttcggcg gagtaagaac ccggataaca atgatgatga tcatcagtta ttttgacgat
    10321 ctgcctgaag gtgaagattt ataaggagtt gtcgatgtca gtacctctca ttctgaccat
    10381 actggcgggg gcagccacgt ttattggcgc gtttctcggc gttctcgggc aaaaaccctc
    10441 gaaccgctta ctggcgtttt cgctaggatt tgcggcgggg atcatgttgc tcatctcatt
    10501 aatggaaatg cttcctgccg cactagcagc tgaaggaatg tcgcctgtgt tgggttatgg
    10561 aatgtttatc ttcggtctgc ttggctattt tggcctggac cgcatgttgc cacatgcgca
    10621 tccgcaggat ttaatgcaaa aatcggtgca gccgttgcca aaatcgatca agcgcacagc
    10681 cattctgctc actctcggca tcagtctgca taacttcccg gaagggattg ccacctttgt
    10741 cacggcgagc agcaacctgg agctgggatt tggcatcgca ctggccgtcg cgttgcacaa
    10801 tattcctgaa ggtctggcgg ttgcaggccc ggtttatgca gcaacgggtt ctaaacgcac
    10861 ggcaattctg tgggcgggga tttctggatt ggcagaaatt cttggtggtg tgctggcgtg
    10921 gttaatcctc ggtagcatga tttcccccgt ggtcatggcg gcaatcatgg cggcggttgc
    10981 aggaattatg gtggcgctct cggttgatga attaatgccg ctcgccaaag agattgaccc
    11041 taataataac cccagctatg gcgtactatg tggaatgtca gtgatgggat tcagtttagt
    11101 gctgctacaa acggcgggaa ttggttaaaa gcaacatcag aagttggcca gcgcatggac
    11161 aacttctgct agcttcacta aagccttatt ctaccttttc ggctgtctct tctctcgtac
    11221 tgtttaagta tttgttccgc gcttcaagca gtgcgtctct gtcctggcga aacgcccgac
    11281 aataacgttt caaatgcagg aaataaccca ccacaacaag agcaatcaat acaatccagt
    11341 accaggcgat aaacatcctt ttacccttaa tatttaacaa tactaaacat ttagcgcata
    11401 aatttcacat atcctttttc ggatatattc atatggtcgc ataataacgg aacaattata
    11461 tgattttgat cataatgaaa aagaatgcct gatattaatg aaattttctc atagaaagag
    11521 aagcggaatt aacatcttgc atagcagggc aaaataaaaa ccgaagccag ctacaacttc
    11581 ggtttcttga ttaaggcagt aattaagcag cggttttcag ctggctttac gctcatgtgc
    11641 ctgacggtat gccaccaggt cttcaatagt cacgagcgcc atattgtgtt tattggcaaa
    11701 ctcaatgcac tctggtgcac gcgccatcgt gccatcgtca ttagtcaact cacacagcac
    11761 accagccggt ttaaagcctg ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc
    11821 acgcgtcagt acaccacctg cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag
    11881 atcagaaggt tttgcgccat cggcaatcgc tgcgcgaacg gtcgtaattc gatcagcggc
    11941 agaaacaccg gtagtcacac cttcagccgc ttcaatggtc acggtaaaac cggtgccata
    12001 ggcgctggtg ttattttcta ccatcattgg cagatcaagt tgtttacggc gatcttcagt
    12061 gatgcacagg caaacaatac cgctaccgtg gcgaatggtc agcgccatct gctcaacggt
    12121 catggtttct gccgggaaga tcatatcgcc ttcgttttca cggtcttcat catcaagcac
    12181 cattacaccg cgtccttcac gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt
    12241 accaaaagag gaaagtagcg tctgattcat ggtaaaaaaa cctcactaaa attatggtta
    12301 ccagaatcag ggcagtctta ggagtggcgg catatagcca aaataacgtg agcgggtcca
    12361 tgcccgacag aatcgttact ctctcccatc cggactctaa ccgtcggccc cggaattaca
    12421 ccggatctgc tgtcctttga gttcgcaccc aaagcgctcg cgggctttca actgagttga
    12481 tttaccgccg gtggggaatt tcgccccgcc ctgagaataa gcggattcac tataacgcta
    12541 atgattagcg gcagcaacgc atagcttcac ataattctgg tttatgactt acccttatcg
    12601 cactacaatg gcactcaaca cctatcagta cagggaaact acaatgattg acccgaaaaa
    12661 aattgagcaa atcgctcgcc aggttcacga atcaatgcct aaaggaatca gggagttcgg
    12721 ggaagatgtg gagaaaaaaa tccgccaaac cctacaagct cagctgacgc gcctcgacct
    12781 tgtaagccgt gaagagttcg acgtccaaac gcaagtcctg ttacggactc gtgagaaact
    12841 ggcgctgctt gaacaaagaa tgagcgaact ggaaaatcgc agtactgaga ttaaaaagca
    12901 gccagatcca gagactctcc ccccaacgct gtaaacattt gtctttaatg cactgtaaat
    12961 tatgaattaa tcctctaatt tacagtgctt tctattacct tatcgcttaa ttccattcag
    13021 aatatcatca caaaaatg
//
LOCUS       DAZFWL020000129        13022 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFWL020000129 DAZFWL020000000
VERSION     DAZFWL020000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.129"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            81..329
                     /locus_tag="ACHKNP_RS19560"
                     /old_locus_tag="pgaptmp_003912"
     CDS             81..329
                     /locus_tag="ACHKNP_RS19560"
                     /old_locus_tag="pgaptmp_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4705996.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            307..1437
                     /locus_tag="ACHKNP_RS19565"
                     /old_locus_tag="pgaptmp_003913"
     CDS             307..1437
                     /locus_tag="ACHKNP_RS19565"
                     /old_locus_tag="pgaptmp_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU4705997.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHKNP_RS19570"
                     /old_locus_tag="pgaptmp_003914"
     CDS             complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHKNP_RS19570"
                     /old_locus_tag="pgaptmp_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HGU4705998.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2361..2450
                     /locus_tag="ACHKNP_RS19575"
                     /old_locus_tag="pgaptmp_003915"
                     /pseudo
     CDS             2361..2450
                     /locus_tag="ACHKNP_RS19575"
                     /old_locus_tag="pgaptmp_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2478..3221
                     /gene="yciC"
                     /locus_tag="ACHKNP_RS19580"
                     /old_locus_tag="pgaptmp_003916"
     CDS             2478..3221
                     /gene="yciC"
                     /locus_tag="ACHKNP_RS19580"
                     /old_locus_tag="pgaptmp_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HGU4705999.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3251..3790
                     /gene="yciB"
                     /locus_tag="ACHKNP_RS19585"
                     /old_locus_tag="pgaptmp_003917"
     CDS             3251..3790
                     /gene="yciB"
                     /locus_tag="ACHKNP_RS19585"
                     /old_locus_tag="pgaptmp_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HGU4706000.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3895..4293
                     /gene="yciA"
                     /locus_tag="ACHKNP_RS19590"
                     /old_locus_tag="pgaptmp_003918"
     CDS             3895..4293
                     /gene="yciA"
                     /locus_tag="ACHKNP_RS19590"
                     /old_locus_tag="pgaptmp_003918"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HGU4706001.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHKNP_RS19595"
                     /old_locus_tag="pgaptmp_003919"
     CDS             complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHKNP_RS19595"
                     /old_locus_tag="pgaptmp_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HGU4706002.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5276..5572
                     /gene="yciI"
                     /locus_tag="ACHKNP_RS19600"
                     /old_locus_tag="pgaptmp_003920"
     CDS             5276..5572
                     /gene="yciI"
                     /locus_tag="ACHKNP_RS19600"
                     /old_locus_tag="pgaptmp_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HGU4706003.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5871..7124
                     /gene="kch"
                     /locus_tag="ACHKNP_RS19605"
                     /old_locus_tag="pgaptmp_003921"
     CDS             5871..7124
                     /gene="kch"
                     /locus_tag="ACHKNP_RS19605"
                     /old_locus_tag="pgaptmp_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HGU4706004.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(7179..7352)
                     /gene="yciY"
                     /locus_tag="ACHKNP_RS19610"
                     /old_locus_tag="pgaptmp_003922"
     CDS             complement(7179..7352)
                     /gene="yciY"
                     /locus_tag="ACHKNP_RS19610"
                     /old_locus_tag="pgaptmp_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HGU4706005.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7495..8955
                     /gene="cls"
                     /locus_tag="ACHKNP_RS19615"
                     /old_locus_tag="pgaptmp_003923"
     CDS             7495..8955
                     /gene="cls"
                     /locus_tag="ACHKNP_RS19615"
                     /old_locus_tag="pgaptmp_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGU4706006.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8990..9319
                     /gene="yciU"
                     /locus_tag="ACHKNP_RS19620"
                     /old_locus_tag="pgaptmp_003924"
     CDS             8990..9319
                     /gene="yciU"
                     /locus_tag="ACHKNP_RS19620"
                     /old_locus_tag="pgaptmp_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HGU4706007.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9372..10376)
                     /gene="oppF"
                     /locus_tag="ACHKNP_RS19625"
                     /old_locus_tag="pgaptmp_003925"
     CDS             complement(9372..10376)
                     /gene="oppF"
                     /locus_tag="ACHKNP_RS19625"
                     /old_locus_tag="pgaptmp_003925"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HGU4706008.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10373..11386)
                     /gene="oppD"
                     /locus_tag="ACHKNP_RS19630"
                     /old_locus_tag="pgaptmp_003926"
     CDS             complement(10373..11386)
                     /gene="oppD"
                     /locus_tag="ACHKNP_RS19630"
                     /old_locus_tag="pgaptmp_003926"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HGU4706009.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11398..12306)
                     /gene="oppC"
                     /locus_tag="ACHKNP_RS19635"
                     /old_locus_tag="pgaptmp_003927"
     CDS             complement(11398..12306)
                     /gene="oppC"
                     /locus_tag="ACHKNP_RS19635"
                     /old_locus_tag="pgaptmp_003927"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGU4706010.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12321..>13022)
                     /gene="oppB"
                     /locus_tag="ACHKNP_RS19640"
                     /old_locus_tag="pgaptmp_003928"
     CDS             complement(12321..>13022)
                     /gene="oppB"
                     /locus_tag="ACHKNP_RS19640"
                     /old_locus_tag="pgaptmp_003928"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HGU4706011.1"
                     /translation="DFGPSFKYKDYSVNDLVASSFPVSAKLGAAAFFLAVILGVSAGV
                     IAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHWLPGGGWNGGALKFMI
                     LPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRRIILRHALKPALLPVL
                     SYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVLSLTILVGALTILFNA
                     IVDVLYAVIDPKIRY"
BASE COUNT         3361 a         3194 c         3105 g         3362 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
       61 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
      121 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
      181 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
      241 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
      301 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
      361 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
      421 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
      481 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc
      541 ggcggttcat caaccgttac catttttctg aataattaca gaaaaattca acaggaaaga
      601 tatgaaaacg gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta
      661 tttgatgaac gttttggcac cagaccgtta gatgccataa ccgtaaaaga tgtggtatca
      721 gtactggaag agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta
      781 ctgatcgatg ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct
      841 gcagaatcgg caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag
      901 tggatgatga tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg
      961 ctggcactga tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc
     1021 cgggatggtt atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg
     1081 gctctgcgtt gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat
     1141 tgcgttctta gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc
     1201 gggatggtta agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat
     1261 tacaactggc gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag
     1321 cgattgttca gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa
     1381 atgaccgata tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga
     1441 ccagttttgc agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc
     1501 ggagaacata aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga
     1561 tttgtactta gccgtggtat cgatatccat gtaccacact gacatgttaa ccaaccagtc
     1621 acggttaatc agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact
     1681 gagatcggaa agccccgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt
     1741 gtagttaata ccggccccaa cgtaaggacg gaatttgctg ctggcatcac caaaatacca
     1801 ctgcgccatc agtgttggcg gcagatggtg aacggttgca atatcgccgg tcgcccgggt
     1861 gccgatttta tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc
     1921 catataagta aacgtcaagc ccagttgagt gttattggtc acgctgaatc cacccagact
     1981 tcctaacgta ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa
     2041 aaattcgcct gcttcatgcg caaaggcact gccagaaaga agagttgtta ctgccaaagc
     2101 cgccactgtt aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata
     2161 tacctacatt ggagtaacaa atgatcctac atagatcaca ttacatatga taattcacca
     2221 tttattgatc tggattaatt tagaagttgc attgagaatc ctggttaatg tgatttaaat
     2281 caatttttaa atattttctc tacgcaaata ttaactcgat cgccacttgc cgcggtttgc
     2341 ccacgcctga aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac
     2401 ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata
     2461 aaactcaagg agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt
     2521 tcttccgtaa tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag
     2581 tggtgttagg gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacggcg
     2641 tgcccgttag cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc
     2701 aacaaatttt gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca
     2761 ttctcgccgg aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg
     2821 cgctgcgagc tattggtgcc agtgcgccga tattgcccaa gttatttatt ctgatttttc
     2881 tgactaccct tttagtacag attggcatca tgctggtggt cgttccggga attatcatgg
     2941 ccattttact ggccctggca ccggtgatgc tggtacagga caaaatgggc atttttgcct
     3001 cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac
     3061 tgagctggtt gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa
     3121 ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa cctgatttct gccatattgc
     3181 tcatctatct gttccgcctg tatatgttga ttcgccaata accttaagca tttgattacg
     3241 gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta
     3301 caagatttat gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct
     3361 tatctatagc tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct
     3421 ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa
     3481 ggttacagtc atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa
     3541 aaagccgcta attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc
     3601 gaagctaaat ctggcctggg ctgttttctt tatcctttgc ggtctggcaa acatctacat
     3661 cgcattctgg ctgccgcaaa atatttgggt caactttaaa gtctttggcc tgaccgccct
     3721 taccttaatc ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga
     3781 taaatcctaa ccagactgcc agtccttcgg ggctggcgct gtatctcctc tgttctcctc
     3841 aggaaaatca tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct
     3901 acaacacata acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat
     3961 accaatgcca atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc
     4021 gctattctgg cgaaagaaat tgcccacggt cgcgtagtga ctgtgcgggt tgaaggaatg
     4081 actttcttac ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag
     4141 aaagggacga catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa
     4201 ccaatcgggc aacgctataa agcgacagaa gcattattta agtatgtcgc ggttgatcct
     4261 gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt
     4321 ttgactttct gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac
     4381 aatcccactg cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc
     4441 acgctcaaac atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc
     4501 cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc
     4561 cggatactgc ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg
     4621 cttgctggtt gcagccgttg ccgtacttga tgtggggcgc gcgggtgccg tattttcaaa
     4681 cggtgatgcc ggacgcgact ctacgggttt gatatcgcgt tttggctgct cctgtacctt
     4741 tttcaccggc ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccaccggtgc
     4801 ttcttttggc ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg
     4861 tggcggctga acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat
     4921 tggctgcgca ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc
     4981 cacaacagca ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa
     5041 atcaagggtc attgaagtca taatcatttc agtagaaaaa ccaggtctcg attttaaatg
     5101 caaatagcaa tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg
     5161 gcgtaattga ccttaattta tgcatggtct taacaaagac atcatctttc cgttgcagat
     5221 cttcgccctt tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt
     5281 gtatgttatt tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc
     5341 ggcacattta gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc
     5401 aatgccagcg gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat
     5461 tgctgaattt gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc
     5521 ggcaggagtc tatgaacacg tttcagtgaa accatttaag aaagtgttct gaatagagat
     5581 tcaatattta agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttatagccg
     5641 gtcagtgcat tcaccaccac aagcagataa taactttatc tgtatttctt tatatgatgt
     5701 gaacatattc aggaaaatgc cctttcctga cgcagagttg ctcgctcaaa acctttgatt
     5761 actgccgata aagtcatcta ctattgttta acacattcac gcgctgcatg tatgttcaga
     5821 aaatgtgcac gctaagataa ttaaacaaaa ttgtggttac agaggtatca gtgagtcact
     5881 gggctacatt caaacaaaca gcaacaaatt tatgggtgac ccttcggcac gacattctcg
     5941 cgctggccgt ctttttaaat ggattgctta tttttaaaac aatctatggt atgtcggtca
     6001 atttgcttga tattttccat atcaaagcat tttcagagtt ggatctctcc ttgctggcaa
     6061 acgccccact atttatgctc ggcgtctttc ttgtcctgaa ctccattggc ttactgttcc
     6121 gggcaaagct cgcatgggca atcagtatca ttttgttgtt gatagcgcta atttacaccc
     6181 tgcattttta tccctggctg aaatttagta ttggattttg catttttacg ctggtgtttt
     6241 tgctgatact gcgcaaagac ttctcccaca gtagcgccgc agccgggaca atttttgcat
     6301 ttattagttt cacgacatta ctgttttact caacctacgg tgcgctttat ttaagcgaag
     6361 gttttaatcc gcgaatagaa agtttgatga ccgcgttcta tttttcgata gaaaccatgt
     6421 caaccgtcgg ctatggcgat attgtccctg tttctgaatc agcacgattg ttcactattt
     6481 cggtcattat ttccggcatt accgtttttg ccacatctat gacctcaatt tttggtccgc
     6541 ttatccgcgg gggattcaac aaacttgtaa aaggaaacaa tcatacaatg catcgtaaag
     6601 atcattttat tgtttgcgga cattcgattc tcgccatcaa tacgatcctg caactgaatc
     6661 aacgcggaca aaacgtaacg gttatcagca acttgcctga agatgatatc aagcaacttg
     6721 agcaacgctt aggcgataac gctgatgtta tccccggtga cagtaatgac agttcagtat
     6781 taaagaaagc gggaatcgat cgttgccggg ccattctggc gctgagtgat aacgatgcag
     6841 ataacgcgtt tgttgtactc tcggcaaaag atatgagcag tgatgtcaaa acagttctcg
     6901 ccgtcagtga tagcaaaaac ctgaataaga ttaagatggt acatccggat atcattctct
     6961 caccgcaact gtttggcagc gaaattctgg cgcgagtttt aaatggtgaa gagattaata
     7021 atgatatgct cgtttcaatg ttgttgaact ccggtcatgg cattttcagc gataacgatg
     7081 aacaagaaac gaaagctgac agtaaagaat cagtgcaaaa atagagttta aagacgaaag
     7141 agtaaactta ttgggcaccg caatggtgcc cagtatattt agatattgta gatcgcaaac
     7201 aataacgagt tacgctgttt gtttagaatg cacttcctga tgctgtggat acgcatattt
     7261 cggcgcgatt gcccctcaag ccaacgcgat ttacgtcggc tagcctgacg ctgcatgcgc
     7321 cagcgcccca cttccgttct actccgcttc atgttaacta ctctatgcaa taacagaatg
     7381 gtcattatac ccccaccccc gacgctgacc agtgcttttc ccgcgttttt atttgccaga
     7441 tgaatccata tgcgtaaact cataacaatg cgctttcaaa aggatttcta atctatgaca
     7501 accgtttata cgttggtgag ttggttggcc attctgggat actggttgct cattgcaggc
     7561 gtaactttac gcattctaat gaaacgacgc gcagttccct ccgcgatggc ctggctgttg
     7621 attatttaca ttctgccgtt agtcggaatt attgcctatc ttgccgttgg cgagctccat
     7681 ttaggcaaac gccgcgctga gcgcgccaga gcgatgtggc cttccaccgc aaaatggctt
     7741 aacgacctta aagcctgtaa gcatatcttc gccgaagaaa atagcagtgt cgctgcgcca
     7801 ttattcaagc tttgcgagcg tcgtcagggg atcgctgggg tcaaagggaa tcagctacaa
     7861 ctgatgaccg agtcagatga tgtgatgcag gcgttaatcc gcgacatcca gctcgcgcgc
     7921 cataatattg agatggtgtt ttatatctgg cagcccggcg gcatggcgga ccaggtggct
     7981 gaatcattaa tggcggctgc acggcgtggt attcattgcc gattgatgct cgactccgcc
     8041 gggagtgtgg cttttttccg cagcccgtgg cccgagctaa tgcgtaatgc cggtattgaa
     8101 gtggtcgaag ccttaaaggt caatctgatg cgtgtgtttt tacgccgtat ggacctgcgc
     8161 caacatcgca agatgatcat gatcgataat tacatcgcgt acaccggcag catgaatatg
     8221 gtcgatcctc gctacttcaa acaagatgcg ggcgtagggc aatggattga tctgatggcg
     8281 cgtatggaag gccccatcgc caccgcgatg gggattattt attcctgcga ctgggagatt
     8341 gaaaccggaa aacgtattct gccgccacca ccagatgtca atattatgcc gtttgaacag
     8401 gccagcggtc acaccattca cacaattgct tctggccccg gctttccgga agatctcatt
     8461 caccaggcat tattgactgc ggcttattcg gcgcgtgaat atttgatcat gaccacgccc
     8521 tactttgtgc caagcgatga tttacttcat gcgatttgca cggcggcgca gcgcggggtg
     8581 gatgtcagta ttatccttcc gcgaaaaaat gactcgatgc tggtcggctg ggccagtcgc
     8641 gcattcttta cggaactgct tgctgctggg gttaaaattt atcagtttga aggcgggtta
     8701 ctgcatacca agagcgtgct ggtcgatggc gaactaagtc tggttggcac cgttaacctt
     8761 gatatgcgga gtctgtggct gaatttcgag attaccctgg caatcgacga taaaggtttt
     8821 ggtgcagacc ttgccgccgt tcaggacgat tatatttcgc gttcacgtct gctcgatgcc
     8881 cgtttatggc taaaacgtcc attatggcaa cgtgtcgccg agcgactgtt ttacttcttc
     8941 agtccgttgc tgtaaaacgt cgccaacaga ggttaaacag gtagtgacta tggatatgga
     9001 tctaaacaat cgcctgactg aagatgaaac gcttgagcag gcctacgata tttttctcga
     9061 actggctgcc gacaatctcg atccagctga tgttctgctg ttcaatcttc agtttgaaga
     9121 gcgcggcggc gcggagttat tcgatccggc ggaagactgg caggaacatg ttgatttcga
     9181 tttgaaccca gacttttttg ccgaagtggt gattggtctg gcggacagtg aagatggtga
     9241 aatcaacgat gttttcgccc gcattctgtt atgccgcgaa aaagatcaca aactttgcca
     9301 tatcatctgg cgggaataaa taatcgcttt tagcgtgaaa aagggctgac aactgtcagc
     9361 ccttattgtg tttaaagcgg atcgactttc aggcaagaaa cggcgtgtct gaaactcccc
     9421 tccagaacag gacgtgtttt ggcgcactcc ggaccggcaa tcgggcaacg ggtacggaaa
     9481 acacaaccgg aaggcgggtt gatcggcgac ggtaattccc cttccagtaa ctggatggtt
     9541 ttgttcttct ccagatccgg atcaggtatg gggactgccg acatcaatgc cctggtgtaa
     9601 ggatgtagtg gattgtggta gacctcatca taggtcccca gttctaccgc atggccgaga
     9661 tacatcacca acacacgatc ggaaatgtgt tttaccacgg ccaggtcatg agcgataaaa
     9721 attaatgaca atcccatctc acgttgcagc tgctggagca ggttgaccac ctgcgcctga
     9781 attgacacgt ccagcgccga aaccggctca tcgcagataa tcagcttcgg ttcaagaata
     9841 agagcacgag caatcccgat acgctggcac tgcccaccgg agaactcatg cggatagcgg
     9901 ttaatcaggt taggcaataa cccgactttc agcatcatcg ccttcacgcg ctcgcgaact
     9961 tcctggcgtg acattttcgg atgataagta cgcagtggtt cagcgatgat ctcgccgatg
    10021 gtcatacgcg ggtttagcga tgccaacgga tcctggaaaa tcatctgaat atcactgcga
    10081 acggcacgcc attcatcggg cttcatgccc agcaactctt tacctaacca ggcaacatga
    10141 ccgtcggtcg ccttgaccaa accgatgatg gcgcgagcaa aggtggactt accgcatccc
    10201 gattccccta ccacacctaa tgtttcccct tcatacaggc gaagagtgac accatcgacg
    10261 gctttgagcg ttttcggcgg ttgccagaac cactgtttgc catctttgat ttcaaagtgc
    10321 actttaagat cggcgatttc aaggaggact tttcttcctt cagttacagc attcataaca
    10381 gttcctccac cggtttaaag caagcacgca gacggccagg cgtaaactct tccagcggcg
    10441 gtgcgctact acaaatttcc atcgcatgtg gacaacgtgg ctggaacggg caacctttcg
    10501 gtaatcgcag caagtttggc ggattacccg ggatggtcaa cattgtttca ccttccgcat
    10561 caagacgcgg caccgcgttg agcaaaccga tagaataagg atgaacgggt tgataaaaga
    10621 catcgcgcgc attgccatat tccatcgtgc gcccggcgta cattaccagc actttgtcac
    10681 agatccccgc caccacgcca agatcgtggg tgatcataat gatggcggta ttaaattccc
    10741 gcttcagttc atttaatagc gtcatgatct gcgcctgtac ggtgacgtcc agcgcagtag
    10801 tgggttcatc cgcaatcagt agcttaggtc gacatagcaa tgccatcgca atcatgactc
    10861 gctgacgcat gccgccagaa aattcatgcg ggtacatttt catgcgttta cgcgcttccg
    10921 gcatttttac cgcatcgagc atccgcaccg actcttcaaa cgcctcagct ttgctcatgt
    10981 tcttatgcag catcagcact tccattaact gctcaccgac gcgcatatag ggattcaacg
    11041 aagtcattgg gtcctgaaaa atcattgaga tttgttcagc gcgcagttta ttgagttcat
    11101 gctctggcaa attgaggatt tcacgcccat tgaaggttgc cgatccgcca atacgtccgt
    11161 tggcagccaa caggcccatc aacgcaaatg cagtttgcga tttacccgaa ccagactcac
    11221 ctacaatacc cagcgtttct ccggcacgta gggaaaaatt caaatcattg accgccgtga
    11281 cgtcgccgtc cggggtacta aaggtgacac gcaaatcttt cacgttcagc agtgcgtcag
    11341 cctgttgttg tgcgagcggc acagttgcag tttcaattac gctcatggct gcactcctta
    11401 acgatctttc gggtcgaggg catcacgcaa gccatcgccg ataaagttga aacaaaacag
    11461 cgtcaccacg aggaatcccg ctgggaacaa caataaccat ggagagactt ccatcgagtt
    11521 cgcgccatca ctcagcaatg caccccagct gcttaacggc tcttgcgtac ccaaccccag
    11581 gaagctaagg aaagattcaa agaggatcat gctgggcacc agaagtgatg cgtagaccac
    11641 caccacaccg agtacattcg gcacaatgtg gcgaataaca atgcccgatg tcgatacacc
    11701 gccaacttgt gccgcctcaa taaactcttt gcgcttcaga ctcagggttt gcccacgcac
    11761 aatacgagcc atatccagcc aggaaaccat gcctatcgcc acgaaaatca gcaggatgtt
    11821 ttgaccgaaa aaggtcacca gcaaaatgac gaagaacatg aatgggaagg agttgaggat
    11881 ttccagcaga cgcatcatta ccgaatccac tttaccgccc agataaccgg aaagcgaacc
    11941 ataaagtgtc ccgacgacca cagccaccag tgccgcagca acaccgacca tgagtgagat
    12001 acgcccgcca atcgcaacgc gcacaagcag gtcgcgaccg gatgagtcag taccaaagta
    12061 gtgaccggac tccatatccg gggcgctgga catcatcgcc cagtcagtat cgtcataggc
    12121 aaactgggaa agcatcggtg ccaggattac aaataacgcg atcagcacca gcactatcag
    12181 actggcaacc gccgcacggt tatgcataaa acgtcgacgt gcgtcctgcc acaagctgcg
    12241 cccttcgacc tccagctttt cactgaaatt ttccagcgtc tcgctgtttt tcttacttaa
    12301 catcatcgcg agctccagta tcagtaacgg attttcgggt cgataaccgc atatagcaca
    12361 tcgacaatgg cattaaacaa aatggttaaa gcaccaacca ggatggtcag gcttaacact
    12421 aaggaatagt cacggttcaa tgcgccatta acgaacagtt gtccaatccc cggcaaacca
    12481 taaatggttt cgataaccat agaaccggta ataatgccga caaatgcggg gcccatataa
    12541 gagagcacgg gtaacagagc aggttttaat gcgtgacgta aaatgatccg ccgcataggt
    12601 aaccctttcg cccgggcagt acgaataaaa ttggagtgta atacttcaat catagagcca
    12661 cgggtaatac gcgcaatact ggcgatataa gcgagtgaca acgccaccat cggcaatatc
    12721 atgaatttaa gcgccccacc attccagcca ccgcccggca gccaatgcaa aatgatcgca
    12781 aatatcatga ctaataatgg tgcaaccaca aaactgggga taacaacccc ggtcattgcc
    12841 agccccatca cggtatagtc ccatttggtg ttttgtttta atgcggcaat aacgccagca
    12901 ctaaccccca gtattaccgc aaggaaaaat gctgcggctc ccagtttggc agaaacggga
    12961 aaactggatg caaccaagtc attgaccgaa taatctttat atttaaacga tggaccgaaa
    13021 tc
//
LOCUS       DAZFWL020000130        12800 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFWL020000130 DAZFWL020000000
VERSION     DAZFWL020000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.130"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            200..1714
                     /gene="caiT"
                     /locus_tag="ACHKNP_RS19645"
                     /old_locus_tag="pgaptmp_003929"
     CDS             200..1714
                     /gene="caiT"
                     /locus_tag="ACHKNP_RS19645"
                     /old_locus_tag="pgaptmp_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HGU4706012.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1745..2887
                     /gene="caiA"
                     /locus_tag="ACHKNP_RS19650"
                     /old_locus_tag="pgaptmp_003930"
     CDS             1745..2887
                     /gene="caiA"
                     /locus_tag="ACHKNP_RS19650"
                     /old_locus_tag="pgaptmp_003930"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HGU4706013.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3016..4233
                     /gene="caiB"
                     /locus_tag="ACHKNP_RS19655"
                     /old_locus_tag="pgaptmp_003931"
     CDS             3016..4233
                     /gene="caiB"
                     /locus_tag="ACHKNP_RS19655"
                     /old_locus_tag="pgaptmp_003931"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HGU4706014.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4307..5860
                     /gene="caiC"
                     /locus_tag="ACHKNP_RS19660"
                     /old_locus_tag="pgaptmp_003932"
     CDS             4307..5860
                     /gene="caiC"
                     /locus_tag="ACHKNP_RS19660"
                     /old_locus_tag="pgaptmp_003932"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HGU4706015.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5969..6754
                     /gene="caiD"
                     /locus_tag="ACHKNP_RS19665"
                     /old_locus_tag="pgaptmp_003933"
     CDS             5969..6754
                     /gene="caiD"
                     /locus_tag="ACHKNP_RS19665"
                     /old_locus_tag="pgaptmp_003933"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HGU4706016.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6760..7350
                     /gene="caiE"
                     /locus_tag="ACHKNP_RS19670"
                     /old_locus_tag="pgaptmp_003934"
     CDS             6760..7350
                     /gene="caiE"
                     /locus_tag="ACHKNP_RS19670"
                     /old_locus_tag="pgaptmp_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HGU4706017.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7436..7831)
                     /gene="caiF"
                     /locus_tag="ACHKNP_RS19675"
                     /old_locus_tag="pgaptmp_003935"
     CDS             complement(7436..7831)
                     /gene="caiF"
                     /locus_tag="ACHKNP_RS19675"
                     /old_locus_tag="pgaptmp_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HGU4706018.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8092..11313)
                     /gene="carB"
                     /locus_tag="ACHKNP_RS19680"
                     /old_locus_tag="pgaptmp_003936"
     CDS             complement(8092..11313)
                     /gene="carB"
                     /locus_tag="ACHKNP_RS19680"
                     /old_locus_tag="pgaptmp_003936"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGU4706019.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11331..12479)
                     /gene="carA"
                     /locus_tag="ACHKNP_RS19685"
                     /old_locus_tag="pgaptmp_003937"
     CDS             complement(11331..12479)
                     /gene="carA"
                     /locus_tag="ACHKNP_RS19685"
                     /old_locus_tag="pgaptmp_003937"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGU4706020.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         3018 a         3320 c         3384 g         3078 t
ORIGIN      
        1 caaaattaac gagaaaagtg ccctctattc acccgaaaca aaaatgtgat aacaatcaca
       61 gaatgcagct tattgaatac ccattatgag ttagccatta acgcgtccac gaggttaata
      121 ataattatat taaatgttaa caaaaataaa acaaacggga acgcaaaata aatattcgtt
      181 ttcacagtgg aattaactca tgaagaatga aaagagaaaa acgggaatag aaccgaaggt
      241 tttctttccg ccgttaataa tcgtcggcat actttgttgg cttacagtca gagatctgga
      301 tgcagcgaat gtcgttatta atgctgtatt cagttacgtc accaatgtat ggggatgggc
      361 atttgaatgg tatatggtgg tgatgctttt cggttggttc tggctggtgt ttggcccgta
      421 tgccaaaaag cgtttaggta acgaaccacc tgaatttagt accgccagtt ggatctttat
      481 gatgttcgcc tcctgtacgt ctgctgccgt actgttctgg ggatcgattg agatctacta
      541 ctacatctcc accccgccgt ttggcttaga accgaactcg acaggggcga aagagttggg
      601 gctggcttac agcttgttcc actggggacc tctgccgtgg gccacttaca gcttcctttc
      661 agtcgccttc gcttacttct tctttgtccg caaaatggaa gtgattcgcc ccagctcgac
      721 actggtgccg ctggtaggtg aaaaacacgc caaagggttg ttcggcacta tcgtcgacaa
      781 cttctatctc gtcgccttga tcttcgcgat gggtaccagt ctgggccttg ccacgccgct
      841 ggtgaccgag tgtatgcaat ggttgtttgg cattccgcat accctgcaac tggacgctat
      901 catcattacc tgctggatta tcctcaacgc catttgcgtc gcttgcggtc tgcaaaaagg
      961 ggtacgtatc gccagtgacg tgcgtagtta cctgagcttc ctgatgctgg gttgggtgtt
     1021 cattgtcagc ggtgccagct tcatcatgaa ctacttcacc gattcggtgg ggatgttgct
     1081 gatgtatctg ccgcgcatgt tgttctatac cgatcccatc gctaaaggcg gcttcccgca
     1141 gggctggacc gtgttctact gggcatggtg ggtgatttat gctatccaga tgagtatctt
     1201 cctcgcccgc atctcccgtg gtcgtactgt gcgtgaactg tgcttcggca tggtgctggg
     1261 gctgacagcg tcaacctgga tcctgtggac tgtactcggt agtaacactc tgctgttgat
     1321 agataaaaac atcatcaaca ttccaaatct gatcgaacag tacggtgtgg cgcgcgccat
     1381 cattgaaacc tgggccgctc tgccactcag caccgccacc atgtggggct tcttcatcct
     1441 ctgctttatt gccaccgtta cgctggttaa cgcctgctct tataccctgg cgatgtccac
     1501 ttgccgcgaa gtacgcgatg gtgaagaacc acctctgctg gtgcgtatcg gttggtcaat
     1561 tctggttggc attatcggta ttgttctgct ggcgctcggc ggcctgaaac cgattcaaac
     1621 cgccattatc gccggaggat gcccgctgtt cttcgtcaac attatggtga cgctctcctt
     1681 tattaaagac gcgaaacaga actggaaaga ttaattaacc cccaaaatat caagaggttg
     1741 aaagatggat tttaatttaa atgatgagca ggaactgttt gtcgccggta tccgcgaact
     1801 gatggccagc gaaaactggg aggcctattt tgccgagtgc gaccgtgaca gcgtctaccc
     1861 ggaacgtttt gtcaaagcac tggcggatat gggtatcgac agtctgctga tccctgaaga
     1921 gcacggtggt ctggacgcgg ggtttgttac tctcgccgcc gtgtggatgg agctgggacg
     1981 tctgggggca ccaacctatg tgctgtacca gttgccgggc gggttcaaca ccttcctgcg
     2041 cgaaggcaca caagagcaga tcgacaagat tatggctttc cgcggcaccg gtaagcagat
     2101 gtggaactca gcgattaccg aaccgggcgc gggctccgac gtgggtagcc tgaaaacgac
     2161 ttatacccgt agaaatggta agatttatct taatggtagt aagtgtttta ttaccagcag
     2221 cgcctacacc ccgtacatcg tggtgatggc gcgcgacggg gcttctccgg acaaacctgt
     2281 ctacaccgaa tggtttgttg atatgagcaa accgggcatc aaagtgacca aacttgaaaa
     2341 gctcggtctg cgtatggata gctgctgtga aatcaccttt gacgacgtgg aactggacga
     2401 gaaagacatg ttcggtcggg aaggtaacgg ctttaaccgc gtcaaagaag agttcgacca
     2461 tgaacgtttc ctggtagccc tcaccaacta cggtacggcg atgtgcgcct ttgaagatgc
     2521 ggcgcgctac gccaatcagc gcgtgcagtt tggcgaggct attggtcgtt tccagttgat
     2581 tcaggaaaaa ttcgcccaca tggcgatcaa attaaactcc atgaaaaaca tgctgtatga
     2641 agcagcgtgg aaagcagaca acggcaccat cacctctggc gatgcagcga tgtgcaaata
     2701 cttctgcgcc aatgcggcat ttgaagttgt ggatagcgca atgcaggtgc tgggcggtgt
     2761 cgggattgcg ggcaaccacc gcatcagccg cttctggcgt gatctgcgtg tagaccgcgt
     2821 ctccggggga tctgacgaaa tgcagatcct gacgctgggt cgcgcggtgc tgaagcaata
     2881 ccgctaagtc cgtgcgctgc ctgatgcgac gctaatgcgt cttatcaggc ctacgggagc
     2941 acgaatgtag gccggataaa gcgtttacgc cgcatccggc agtcatgcgc tacataaatt
     3001 tttcaggagc taataatgga tcatctaccc atgccgaaat tcgggccgtt ggccggattg
     3061 cgcgttgtct tctccggtat cgaaatcgcc gggccgttcg ccgggcaaat gttcgcagaa
     3121 tggggtgcgg aagttatctg gatcgagaac gtcgcctggg ccgacaccat tcgcgttcaa
     3181 ccgaactacc cgcagctctc ccgccgcaat ttgcacgcgc tgtcgttaaa tattttcaaa
     3241 gatgaaggcc gcgaagcgtt tctgaaatta atggaaacca ccgatatctt catcgaagcc
     3301 agtaaaggtc cggcctttgc ccgtcgtggc attaccgatg aagtactgtg gcagcacaac
     3361 ccgaaactgg ttatcgctca cctgtccggt tttggtcagt acggcaccga ggagtacacc
     3421 aatcttccgg cctataacac catcgcccag gccttcagtg gttacctgat tcagaacggt
     3481 gatgttgacc agccaatgcc tgccttcccg tataccgccg attacttttc tggcctgacc
     3541 gccaccacgg cggcgctggc agcactgcat aaagtgcgtg aaaccggtaa aggcgaaagt
     3601 atcgacatcg ccatgtatga agtgatgctg cgtatgggcc agtacttcat gatggattac
     3661 ttcaacggcg gcgaaatgtg cccgcgcatg agcaaaggta aagatcccta ctacgccggt
     3721 tgcggtctgt ataaatgcgc cgacggctac atcgtgatgg aactggtggg cattacccaa
     3781 attgaagagt gctttaaaga tattggcctc gcacatctgc tcggtacgcc ggaaatcccg
     3841 gaaggcactc agcttatcca ccgtatcgaa tgcccttacg gcccactggt agaagagaaa
     3901 ctcgatgcct ggctggcggc acataccatc gcagaagtta aagaacgctt tgccgaactg
     3961 aatatcgcct gtgccaaagt actgaccgta ccggaactgg aaagcaatcc acagtatgtt
     4021 gcccgtgaat caatcactca gtggcaaacg atggatggtc gcacctgcaa agggccgaac
     4081 atcatgccga aattcaaaaa taaccccgga caaatctggc gcggaatgcc ctcacatggc
     4141 atggacacgg ctgccatttt gaaaaatatc ggctacagcg aaaacgacat tcaggagttg
     4201 gtcagcaaag gtctggccaa agttgaggac taaacgcgtg gcgtcagtcg gcttgcctga
     4261 ctagcgcccg cccaataaca aacgaacaaa aatggataga ggtgcaatgg atatcattgg
     4321 cggacaacat ctacgtcaaa tgtgggacga tctggcggac gtttacggtc ataaaacggc
     4381 gctgatttgt gaatccagcg gcggagtcgt taaccggtat agttatcttg agttaaatca
     4441 ggagattaac cgcacggcaa acctgtttta tacgctgggg attcgcaaag gcgacaaggt
     4501 tgcactacat ctcgacaact gcccggaatt tatcttttgc tggttcgggc tggcaaaaat
     4561 tggcgcgatt atggtgccga ttaacgcccg cctgttacgc gaggaaagcg cgtggatcct
     4621 gcaaaatagc caggcgtgcc tgctggtgac cagtgcgcaa ttctatccta tgtatcaaca
     4681 gattcagcag gaagatgcca ctcaattgcg gcacatttgc ctgacagatg tggcacttcc
     4741 cgctgatgat ggcgtgagtt cgtttactca actgaaaaat caacaacctg ccaccttgtg
     4801 ctatgcaccg ccgctattga ctgacgatac ggcggaaatt ctcttcacct ccggcaccac
     4861 ctcccgaccg aaaggtgtgg tgattaccca ttacaacctg cgcttcgctg gatattactc
     4921 cgcctggcag tgtgcactgc gtgacgatga cgtctacctg acggtaatgc ctgcgtttca
     4981 tatcgattgc cagtgtactg cggcgatggc ggcgttttct gccggggcca cctttgtgct
     5041 ggtcgagaaa tacagcgccc gcgccttctg gggacaggtg cagaagtacc gcgccaccat
     5101 taccgaatgt attccgatga tgatccgtac gttgatggtg cagccgcctt cagcgaacga
     5161 tcggcaacac cgcctgcggg aagtgatgtt ttatctcaac ttgtcggagc aggaaaaaga
     5221 tgcgttttgt gaacgcttcg gcgttcgctt gctgacgtct tatgggatga cggaaaccat
     5281 tgtgggcatt atcggcgatc gccctggcga taaacgacgc tggccgtcga ttggtcgggc
     5341 ggggttttgc tacgaagcgg agatccgcga cgatcacaat cgcccgctcc cggcaggtga
     5401 gatcggtgaa atctgtatta aaggcgtacc agggaaaacc atcttcaaag agtattttct
     5461 caacccgaaa gccactgcga aagtgctgga agccgacggc tggctacata ccggcgatac
     5521 cggatactgc gacgaagagg gcttttttta tttcgtcgat cgccgctgca atatgatcaa
     5581 acgcggtggc gagaatgtct cctgcgtgga gctggaaaat atcatcgcca cccatccaaa
     5641 aattcaggat atcgtggttg tgggtattaa agattcgatt cgcgatgaag ccatcaaagc
     5701 atttgtggtg ctgaatgaag gtgaaacatt gagcgaagag gaatttttcc gcttctgcga
     5761 acaaaatatg gcgaaattta aagtgccctc ttatctggag atcagaaaag atctgccacg
     5821 taattgctcg gggaaaataa ttagaaagaa tctgaaataa atgaaacagc agggaactac
     5881 cctgccagct aataatcacg ctataaaaca atatgcattt tttgcaggga tgttgtcgtc
     5941 cctgaaaaag caaaaatgga gaaaaggaat gagtgaatca ttacatctga cccgcaatgg
     6001 atcaattctg gaaattaccc ttgatcgtcc aaaagcgaat gctattgatg caaaaaccag
     6061 ctttgaaatg ggcgaagtat ttctaaattt ccgtgacgat ccgcaattac gtgtcgccat
     6121 tattaccggt gccggagaga agttcttttc cgcgggctgg gatttaaaag cggcagcaga
     6181 aggcgaagca ccggatgctg actttggccc gggtggattt gcgggattaa ccgaaatttt
     6241 caatctcgac aaaccggtta tcgcagctgt gaacggctat gcctttggcg gcggtttcga
     6301 actggcgctg gcggcagatt ttattgtttg tgccgataac gccagcttcg ccctgccgga
     6361 agccaagctg ggcatcgttc ctgacagcgg cggcgtgctg cgtctaccga agatcctgcc
     6421 gcctgccatc gtcaatgaaa tggtgatgac cggcagacga atgggcgcag aagaggcgct
     6481 gcgttggggg atagtcaacc gcgtggttag ccaggcggaa ctgatggata acgcccgcga
     6541 actggcgcag cagctggtta acagcgcccc gctggcgatt gcggcgctga aagagatctt
     6601 ccgcaccacc agcgaaatgc cggtagaaga agcgtatcgc tatattcgca gcggcgtgtt
     6661 gaaacactat ccgtcggttc tgcattcgga ggatgccatt gaagggccgc tggcgtttgc
     6721 cgagaagcgc gatccggtgt ggaaaggacg ttaacaaccg tgagctatta cgcctttgag
     6781 gggttaattc cggtggttca cccgacggcg tttgtccatc ccagtgccgt cttgattggc
     6841 gatgtgattg tgggagccgg tgtctacatc ggcccactcg cctcactgcg tggtgactac
     6901 gggcggttga tcgtgcaagc gggagccaat attcaggatg gctgcattat gcatggctac
     6961 tgcgacactg acaccatcgt tggggaaaac ggccatatcg ggcacggagc gatcctgcat
     7021 ggttgtgtga ttggtcgcga tgcattggtc gggatgaaca gcgtgattat ggatggcgcg
     7081 gtcattggcg aagagagcat tgttgccgcc atgagctttg tcaaagcagg ctttcacggc
     7141 gagaaacgcc agttgttgat gggtacgcca gcccgcgctg tacgcagtgt tagtgacgac
     7201 gagttacact ggaaacggtt gaataccaaa gagtatcagg atcttgtcgg gcgctgtcat
     7261 gcatcgttac atgaaacaca gccactgagg caaatggagg aaaatcgccc ccgtttgcag
     7321 gggacgacgg atgtgacgcc taaacggtga tcttgccgga tgcgacctgg ttgctggata
     7381 agatgctaca acatcgcatc tggcaagcag gacgcccggt atcaacgaga taaaattaac
     7441 gacgcatact ctttgacaac atcatctgcc acttctgctc gcggttaagt tccgtgagcg
     7501 gaacggcagg aatacgcggc gtctttctta cgccgactaa tttatcccgg ctgttattgc
     7561 gcaggcgcgc gtaaatttgc tcatcgatat cgaccacttt taccagtcgc tggcactggc
     7621 atccccgtcc ttccagctta ttagggatca tcttaacttc gcagcttatt tctgcgactt
     7681 ccgacagaat ataagtcagg gtattaaccg ccttgctgtg ttcaatatcg aaatgaatag
     7741 agatctctct tgctatcacc caccgatttt cagccatcat ccattcggcg attaacaagt
     7801 agagtggttt ttcaacatat ccttcacaca tacgaactcc agtttcacaa attaaaagtc
     7861 tggctatgac tcgttttgaa gcaattaaac aaggccatat taaaattgtg gatatatcga
     7921 tagtatgcaa cttcaccgtc gataagtgtg agctaaatcc tgcttatgtc atcaaaaata
     7981 tcgattcccc aaaagattta cttaatgggc aaaaaaaacg gtcctcatca gagaaccgta
     8041 ttacttattc gatcaaatta gcggatgaaa aatatccgcc atgacacgct attatttgat
     8101 ctgcgcgtgc atttcctgca ccgaaattac tttttcggtc gcgtcggcat tcagcgccat
     8161 cgcggtagcg aaaccaccgt tcagggtggt gtcataatgc actttatatt gcagcgcact
     8221 gcgacggatc acgcgggagt cttcaatcgc gcgacggcct gaagtggtgt tgatgatgta
     8281 ggtatattcg ccattcttga tgcggtcctg aatgtgcgga cggccttcat gcaccttgtt
     8341 caccagacgc ggattaatgc ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc
     8401 cagctcgaag ccctgtttca gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc
     8461 ttcgcgcacg gaaagcaacg cacgaccgtg tttcttcatg gtggagttgc tgcccagctg
     8521 cgctttggca aacgcttcag cgaaggtgcg gcccacgccc atcacttccc cggtggagcg
     8581 catttctggc cctaacagcg ggtcaacacc cgggaattta ttgaacggca gcaccacttc
     8641 tttcaccgag tagtacggcg ggataacttc tttggttacg ccctgctcag ccagcgattt
     8701 gccagccatc acacgcgccg ccacttttgc cagcggtacg ccggtggctt tggagacgaa
     8761 cggaacggta cgcgccgcac gcgggttaac ttcaatcagg tagacttcgt tgtttttcac
     8821 cgcaaactgc acgttcatca gaccgcgcac ctgcaattcg aaggccagtt tctgcacctg
     8881 ctggcgcatc acatcctgaa tttcctgact taaggtgtag gctggcagag aacatgcgga
     8941 gtcaccggag tgcacgcccg cctgctcgat gtgctccatg atgccgccaa tcagcaccat
     9001 ttcgccgtcg cagatggcat ccacgtcaac ttctaccgcg tcatcaagga agtgatccag
     9061 caacactggc gcatcgttag acacgctgac cgccgtctgg aagtagcgac gcaggtcagc
     9121 ttcgtcatag acgatttcca tcgcccgacc gccgagaacg taagacggac gtaccaccag
     9181 cgggtagcca atctctttcg ccttctctac cgccatttca atagcggtaa cggtggcgtt
     9241 cgccggttgt ttcagtttca gacgctcaac cgcatgctgg aagcgttcac ggtcttctgc
     9301 acggtcgata gcatccgggc tggtgccgat aaccggtacg ccagcagctt ccagcgcgcg
     9361 cgccagtttc agcggggtct gaccgccgta ctgaacgata acgcctttcg gcttctcaat
     9421 acgcacgatt tctagcacat cttccagtgt taccggctcg aagtagaggc gatcggaagt
     9481 gtcgtagtcg gtggagacgg tttccgggtt acagttaacc ataatggttt cgtaaccgtc
     9541 ttcgcgcagt gccagcgagg cgtgtacaca gcagtagtcg aattcgatac cctgaccgat
     9601 acggttcggg ccgccgccga gaaccatgat tttttcacgg tcggtagacg gattcgcttc
     9661 gcactcttct tcataagtgg agtacatgta agcggtgtcg gtggcgaact ctgccgcaca
     9721 ggtatccacg cgcttataaa ccgggtgcag gtcatactgg tcgcgcagct tgcggatttc
     9781 cgcttcgcgc acgcccgcca gttttgccag acgcgcatcg gcaaagcctt tgcgtttcag
     9841 ctggcgcagg aattcagcgt tcaggccagt gatgcccact tccgctactt tctcttccag
     9901 acgcaccagt tcttcaatct gtaccaggaa ccagcggtca atattggtca ggttgaacac
     9961 gccgtccaca gacaggcccg cacggaaggc atcggcgatg taccagatac gctctgcgcc
    10021 agcatctttc agttcgcgac ggattttggt taacgcttcc gggtcatcga ggctcacttt
    10081 cgggtcgaat ccagtcgcac cgacttccag gccgcgcagc gctttttgca gggattcctg
    10141 ctgcgtgcga ccaatcgcca tcacttcgcc aaccgatttc atctgagtgg tcagacggtc
    10201 gttagcacca gcgaattttt cgaagttgaa gcgaggaatt ttagtaacca catagtcgat
    10261 ggacggctcg aaggaggccg gagtacgtcc gccagtgatg tcgttcatca gttcgtcgag
    10321 ggtgtaaccc accgccagtt tcgccgccac tttagcaatc gggaaaccgg tcgctttcga
    10381 cgccagcgcc gaagaacggg acacgcgcgg gttcatttcg ataacaatca ggcgaccgtt
    10441 tttcgggttc accgcaaact ggacattgga accaccggtt tcaacgccga tttcacgcag
    10501 caccgccatc gaggcgttac gcatgatttg atattctttg tcggtcagcg tttgggctgg
    10561 cgcgacagtg atggagtcac cggtatggat gcccatcgca tcaaagtttt cgatagagca
    10621 gacgatgatg cagttgtcgt ttttatcacg caccacttcc atctcgtact ctttccagcc
    10681 aatcagcgac tcatcaatca gcaactcttt ggtcggagag agatccagac cgcgggcgca
    10741 aatttcttca aactcttcgc ggttataagc gataccgccg ccgctaccgc ccatggtaaa
    10801 ggatgggcga ataatgcacg ggaagcccac gtcagcggca accgccagcg cttcttccat
    10861 cgtgtgtgcg ataccggaac gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa
    10921 acgacggcgg tcttctgctt tatcaatcgc atcggcggtc gcaccaatca tagtcacgcc
    10981 gaactcttcc aacacgccct gacgctccag ctccagcgcg cagttcagcg ccgtctgacc
    11041 gcccatcgtt ggcagcaccg cgtccgggcg ctctttttca ataatcttgc gtaccacttc
    11101 ccagtgaatc ggctcgatgt aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc
    11161 cgggttggag ttcaccagaa tgacccggta accctcttcg cgcagggctt tacatgcttg
    11221 cgcgccagag tagtcaaact cacacgcctg accgataaca atcgggcccg cacccagaat
    11281 caggatactt tttatatctg tacgttttgg catggctctt ttactcctga ttacttagcg
    11341 gttttacggt actgctcaat taactcgata aagtggtcga acaacggcgc ggcgtcgtgt
    11401 ggaccagggc tggcttcagg gtgcccctgg aagctgaacg ccggtttatc ggtgcgatga
    11461 atgccctgta acgtaccgtc gaacagggat ttatgcgtga cacgcaggtt tgctggtaat
    11521 gttgcttcgt ccaccgcaaa accgtggttc tgggcggtga tcatcaccac gtttttctca
    11581 acatctttaa ccggatggtt gccgccgtgg tgaccaaatt tcattttgac agtcttcgca
    11641 ccgctcgcca gcgccagcag ctgatgaccg aggcagatgc cgaagaccgg aatatcggtt
    11701 tcgaggaatt tctggatggc ggtaatggcg taatcgcacg gggccgggtc gccaggaccg
    11761 ttggagagga agataccgtc tggattcatt ttcagcacat cttccgcaga agtttgcgcc
    11821 ggaacgatgg tcaggcgaca gcctctgtcc accaacatgc gcaggatgtt gcgcttggca
    11881 ccaaaatcat aagccacgac gtggaacggc agctcgtctt cttttttcgc ttctggcagg
    11941 ccgccggtca acgtccagct cccttgtgtc cagctatagg cttctgcggt ggtcacttct
    12001 tttgccagat ccatgccatt cagacctggg aacgcgcggg ctttttctaa cgccaacgcc
    12061 gcatccgggt tatcgcctgc gataatgcag ccattctgtg cgcctttctc gcgcagtaaa
    12121 cgcgtcagct tacgggtatc gatatcggca atcgccacga tgttatggcg cttcaggtaa
    12181 gaagagaggt cttcggtatt acggaagttg ctggcaatca gcggcaggtc gcgaatcacc
    12241 agaccttgtg catgtacctg agaagattct tcatcggcgt cattagtgcc gacattgcca
    12301 atatggggat aagtaagagt aacgatttga cgagaatagg aaggatcagt gaggatttct
    12361 tgataaccgg tcattgaagt attgaaaacg acttccccaa ccgccgaacc tgttgcccct
    12421 atggcccgac cgtgaaactg ggttccgtct tccagaacca atagcgctga cttaatcaaa
    12481 acaccctcca gagaatattc actcacttta tttgcatatt aattcacaat gatgacgtaa
    12541 atcaatgcaa atctgcttac cgacgaattt ctggcaaacg gcggcattct ggagatatgg
    12601 gcgctaaaag tcaacttaat ggcgggattt tttatattat tttgtggctc atgcatgttt
    12661 tacaggcatt aagcggcaaa aagatcatat aacctgttct ggaaaacgct tgcgcagcaa
    12721 gaagattgca tgaaataaaa gcgagtaggg aaaagtggac caaatggtca aaatttacat
    12781 taaaacaaca aaaaccgaag
//
LOCUS       DAZFWL020000131        12542 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFWL020000131 DAZFWL020000000
VERSION     DAZFWL020000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.131"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(230..1654)
                     /gene="patD"
                     /locus_tag="ACHKNP_RS19690"
                     /old_locus_tag="pgaptmp_003938"
     CDS             complement(230..1654)
                     /gene="patD"
                     /locus_tag="ACHKNP_RS19690"
                     /old_locus_tag="pgaptmp_003938"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HGU4706021.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1676..2470)
                     /gene="ydcV"
                     /locus_tag="ACHKNP_RS19695"
                     /old_locus_tag="pgaptmp_003939"
     CDS             complement(1676..2470)
                     /gene="ydcV"
                     /locus_tag="ACHKNP_RS19695"
                     /old_locus_tag="pgaptmp_003939"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4706022.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2460..3401)
                     /gene="ydcU"
                     /locus_tag="ACHKNP_RS19700"
                     /old_locus_tag="pgaptmp_003940"
     CDS             complement(2460..3401)
                     /gene="ydcU"
                     /locus_tag="ACHKNP_RS19700"
                     /old_locus_tag="pgaptmp_003940"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4706023.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPRLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(3402..4415)
                     /gene="ydcT"
                     /locus_tag="ACHKNP_RS19705"
                     /old_locus_tag="pgaptmp_003941"
     CDS             complement(3402..4415)
                     /gene="ydcT"
                     /locus_tag="ACHKNP_RS19705"
                     /old_locus_tag="pgaptmp_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4706024.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(4433..5578)
                     /gene="ydcS"
                     /locus_tag="ACHKNP_RS19710"
                     /old_locus_tag="pgaptmp_003942"
     CDS             complement(4433..5578)
                     /gene="ydcS"
                     /locus_tag="ACHKNP_RS19710"
                     /old_locus_tag="pgaptmp_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HGU4706025.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(5823..7228)
                     /gene="ydcR"
                     /locus_tag="ACHKNP_RS19715"
                     /old_locus_tag="pgaptmp_003943"
                     /pseudo
     CDS             complement(5823..7228)
                     /gene="ydcR"
                     /locus_tag="ACHKNP_RS19715"
                     /old_locus_tag="pgaptmp_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            complement(7307..7723)
                     /gene="hicB"
                     /locus_tag="ACHKNP_RS19720"
                     /old_locus_tag="pgaptmp_003944"
     CDS             complement(7307..7723)
                     /gene="hicB"
                     /locus_tag="ACHKNP_RS19720"
                     /old_locus_tag="pgaptmp_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HGU4706026.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(7769..7945)
                     /gene="hicA"
                     /locus_tag="ACHKNP_RS19725"
                     /old_locus_tag="pgaptmp_003945"
     CDS             complement(7769..7945)
                     /gene="hicA"
                     /locus_tag="ACHKNP_RS19725"
                     /old_locus_tag="pgaptmp_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HGU4706027.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            8167..8397
                     /gene="yncJ"
                     /locus_tag="ACHKNP_RS19730"
                     /old_locus_tag="pgaptmp_003946"
     CDS             8167..8397
                     /gene="yncJ"
                     /locus_tag="ACHKNP_RS19730"
                     /old_locus_tag="pgaptmp_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HGU4706028.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(8489..10450)
                     /gene="rlhA"
                     /locus_tag="ACHKNP_RS19735"
                     /old_locus_tag="pgaptmp_003947"
     CDS             complement(8489..10450)
                     /gene="rlhA"
                     /locus_tag="ACHKNP_RS19735"
                     /old_locus_tag="pgaptmp_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HGU4706029.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFFANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(10523..11059)
                     /gene="sutR"
                     /locus_tag="ACHKNP_RS19740"
                     /old_locus_tag="pgaptmp_003948"
     CDS             complement(10523..11059)
                     /gene="sutR"
                     /locus_tag="ACHKNP_RS19740"
                     /old_locus_tag="pgaptmp_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HGU4706030.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            11112..12326
                     /gene="ydcO"
                     /locus_tag="ACHKNP_RS19745"
                     /old_locus_tag="pgaptmp_003949"
     CDS             11112..12326
                     /gene="ydcO"
                     /locus_tag="ACHKNP_RS19745"
                     /old_locus_tag="pgaptmp_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HGU4706031.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            complement(12366..>12542)
                     /locus_tag="ACHKNP_RS19750"
                     /old_locus_tag="pgaptmp_003950"
     CDS             complement(12366..>12542)
                     /locus_tag="ACHKNP_RS19750"
                     /old_locus_tag="pgaptmp_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU4706032.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     PLKQEPTEMIQATA"
BASE COUNT         3076 a         3358 c         3062 g         3046 t
ORIGIN      
        1 ttgtacaatt gaactttcat tgttaatact tctacttata aattattagt ttaataacga
       61 tactctaaag ccttatgcta atcaactgtg acaggatgaa aaatcgccat caaacattta
      121 gcctaatttt tacgctagtt attaatgtaa tcattctaat acttaaccgc cattaaactc
      181 agaacctaaa acgatagtgg ccgtcctata acagccacta acatctaact taatgtttaa
      241 ccatgacgtg gcggacgacg gtgtaatcct ccagcccata aagtgacata tccttgccgt
      301 aaccagaaag tttctgccca ccgtgcggca tttcacttac cagcatgaaa tgggtattga
      361 cccaggtaca accatattgc agccgtgcgc tgacgcgatg cgccctgccc acatctttcg
      421 tccataccga agatgcaagt ccgtactggc tgtcattcgc ccagttcacc acctgttctt
      481 cgttgtcgaa gggcgtaaca ctcactactg gaccaaatac ctctttttgc acgatggcat
      541 cgtcctgtaa tgcgccagcc agcagcgtcg gcgcatagta atagccatta cccttgcgct
      601 tttcaccgcc agtgatcact ttgatgtgcc ctgtcgcttt cgcctcttct actgccttgc
      661 tgacgcgttc gagatgcgcc agcgagctta aaggtccaag ctccgtagac tcgtcatccg
      721 gcgcaccaga ttttaacgtt gccaccgcag cacccagttt ttccaccagc gtatcgtaaa
      781 tgcctttttg cgcgtagatc cgacaagccg cagtacaatc ctgtccagca ttgtaatagc
      841 caaatgtacg tacaccttcg accactgctt caatatccgc atcatcaaaa acaatcactg
      901 gcgctttgcc accaagttcc atatgagtac gcttaatgga cgacgcggta tggctgatga
      961 tgtgctcgcc ggtggcgata gagcccgtca gcgacaccat ccgcactttg ggatgaccgg
     1021 tcagcggatc acccaccgtt ttgcctctgc caaacagtac gttaatcacg cctgccggga
     1081 agatatcttt cgccagctct gccaacttca acgcggtcag cggggtaatt tctgatggtt
     1141 taagcactac gcagttccct gccgccagcg ccggagccag tttccacgct gccatcatca
     1201 gcggataatt ccacggtgcg atagaagcca cgacccccaa cggatcgcga cggatcatcg
     1261 aagtatgacc ttcaagatat tcacctgccg ccagaccatt cagacagcgc gccgcacccg
     1321 cgaaaaagcg aaaaacatcg acaatcgccg ggatttcatc attgaacgca ctatgcagcg
     1381 gtttgccaca attacgggac tccagttcgg caaaaacctg accattttct tcgataacat
     1441 cagccagttt cagcagacat tccgcacgca ctttcggcgt ggtttgcccc cattcggcaa
     1501 atgctgcatc tgccgcgcgc acagcagcat cgacctgctc tgcggatgcc tcggcaatct
     1561 ccagtaaaac gtcccccgtt gccggattat agacaggctg tttttcccct tcgccgctaa
     1621 ccagttctcc gttaatcagt aacttatgtt gcatagcata ttcctgtatc agtggttatt
     1681 gaccattgtc gccttcgcgg gttagccacc aggcccccag gatcggcaag gttgttacca
     1741 acataaccag cagtgccacc acgttagtta ccggtacatc acgcggtcgc ccaagctgat
     1801 tgagcaacca caacggtaac gttcgttcat gacctgccgt aaaagtcgta acgatgattt
     1861 catcgaacga caaggcaaac gccagcattc ctcctgccag tagcgccgaa ctgagattcg
     1921 gcaacactac gtagcggaag gtttgccagc cattggcccc aagatccatt gacgcctcaa
     1981 ccagactcca cgaggtgcgg cgaaaacggg cgatgacatt gttaaacacc accactacgc
     2041 aaaaagtcgc atgaccgacc acgatggtga aaaatcccgg ctccagattg atggttttaa
     2101 aagcggttaa tagcgccaga ccagtgacaa tgcccggcag cgcaatgggc agcagcagta
     2161 acagcgaaat ggcgtttttg ccgaaaaagt ctcgtcgcca cagcgcggca gctgccagcg
     2221 tccctaacac cagcgccatt aatgtcgcca gcgccgccac tttaagtgac agtgtcacgg
     2281 catcaagaat atcactacgc tgtgctgcca cgctaaacca gcgcagcgtc aggccctgcg
     2341 gtggaaaact aaacgccgca tcttcagtgt taaacgcata agcggcgata atcaggatgg
     2401 gaaaatgtag gaaaaccacg ccgccccagg ccgccagttt gaggaaaaac ggtgcgcgtt
     2461 cagagtgcat cgaacgctcc cagacgtttc acgaacgcca ggtacagtgc gatgagaaca
     2521 atcggcacca gggtgaatgc cgccgccatc ggcatattgc caatcgcccc ctgctgggaa
     2581 taaaccatat tgccgataaa atatcctgga ggaccaacca gctgcgggac gataaaatcg
     2641 cccagtgtga gtgagaaggt aaagatagag ccagcggcaa tacccgggat tgccagcggc
     2701 agcaccacat agcgaaaggt ttgtcgtgga cgtgcgccga gatcagccga cgcctgcaac
     2761 aatgacggcg gcaaacgctc aagcgccgcc tgaacgggca ggatcatgaa cggcaaccag
     2821 atatagagaa acaccagaaa gcgccccagc ccggaagttg acagcgtatt tccgcccacc
     2881 gcaggtaatg taaggaacgc agtcagcagt ggttccagcc caagatgttg taaaaaccac
     2941 tgagccacgc catcttttgc cagcaataac gtccaggcat aggctttaac aatgtagctc
     3001 gcccacatcg gcaacattac cgcaatataa aaaaaagctt tcattttccc gctggtatag
     3061 cgcgccatat accacgccat tggaaaagcc agaatggcgc tggcgatagt gactgccaca
     3121 gccatagtca gcgtgcggag aatgatgtcg taattcgccg gattaaacag cgcacggata
     3181 ttcgccagcg tcagttccgg cgttaccgac atggtgaaat cgtcaaaagt ataaaatccc
     3241 tgccataaca gtgtcagcag cgagccgaaa tagacaatgc caaaccacat tagcgggcca
     3301 agcagcagta aaaacagccc cagcctcgga ttacgccaga agaaaccgga caccttaccc
     3361 agacctggac gtgaaggtga ttgcaataca ttcatcgcca ttcacctctc ctcaaccagc
     3421 ggcaccatca catcacgcga ccaggaaacc atcacctgtt gtccgggcgt gagcgtggca
     3481 ggcagttctt cgcctgtcat attggcctga ctcacaagca gtttttcacc gccgttcaat
     3541 ttcagttcaa aacgagttgc cgcgccctga tattgcaccg cctggatcgt gccattggcc
     3601 tgcagttcac caggggtgtt gaggcggata tgttccggtc gcagggcgaa gcttcccgtc
     3661 atgccacaaa gtttctctgc catcagtcca tcaaaaacgt tggatgtccc gacgaatccg
     3721 gcaacaaacg gcgtgcgcgg gcgcatatag agatcgcgcg gggaatcgac ctgctcaatg
     3781 cgtccattat tgaaaaccgc cacacgatcg gacatcgata acgcttcgcc ctgatcgtgg
     3841 gtaacgaaga taaaagtgat accgagagac tgttgcagtt ttttcagttc cagctgcatc
     3901 tgctcacgca atttgagatc cagtgcgccg agcggttcat ccaacagcaa tacgcgcggt
     3961 tcattcacca atgctctggc gatagcaacc cgctggcgct gaccaccaga aagttgtgac
     4021 ggtttacgtt gatgtacaaa ccccaacgcc actttctcca gcgcctcttg cgccattgcg
     4081 tgccgctgct ttttattcac gcctttgacc atcagcccat aggcgacatt gtcaagaatc
     4141 gacatatgcg gaaatagcgc gtagtcctga aagacagtat tcacgtcccg ctcccacggt
     4201 ggcagattgc tggcgggttt accaaagata gagatagccc cgccggaaag ctgttcgaag
     4261 ccagcaatca ggcgcaggca ggtggttttg ccggagccgg acggccccag catagagaag
     4321 aactcaccat cttttatcgc aatactgacg ccatctactg cgcgcacgtc accgtacaac
     4381 cgcgagacgt tgtcaaactc cactgcgtac gtcataaagc accccagcga agttagcgac
     4441 cgcccataat ggcaatgtaa tcctgcgtcc agcgactgta gggaacaaac ttgccccctt
     4501 ctgctatagg cgttttccag aaggcgattt tgtcgaaata gttaaaaccg ttggtttcac
     4561 aacctttttc gcctaataac ggactggctt tacacccttc cggcactacc ggtaacgagc
     4621 caaaccaggc cgccacatcg ccctgcactt ttggcgttaa tgaccagttc atccatttgt
     4681 aggcgcaaac cggatgtttc gcttcgctat gcagcatggt ggtatcagcc caaccggtaa
     4741 caccctcctt cgggaaaacg gtagcaacag gctggccttc ggctttcagg gcgttggcct
     4801 gatagggcca ggcactggaa gcaaccacac cctcgttttt gaaatcgctc atttgcacgg
     4861 tagtgtcatg ccagtagcga tggatcaaac tgtgttgagc gcgcagcact ttcagcaccg
     4921 cctggtactg ttcttcggtt agttgatacg gatcgctgat gcccaactgc ggctgagtgg
     4981 ctttaacgaa caacgcagcg tccgcaatgt agatagggcc atcataagcc tgaacgcggc
     5041 ctttattgct cttgccgtcc ggcagatttt gctcaacaaa aaccacttgc cagctatccg
     5101 gcggcgtcgg gaaggtttta gtgttgtaca tcagcaggtt cggcccccat tggtaaggtg
     5161 tgccgtaaac tttgccgcca acattaaacc agtcgccttt aaccacgcgc ggatcgagcg
     5221 ttttccagtt gggaatcaag gcggtattaa tcggctgcac gcgtttgccc ataatcaaac
     5281 gcagcgaggc atcgccggat gccgtaacca gatcgtaacc ccctttggtc atcagactga
     5341 ccatttcatc ggaagtcgcg gcggttttca cattcaccgc gcagcctgtc tctttttcaa
     5401 attgcgttac ccagtcgtat tgtttatcag tttgtccgcg ttcgatgtat cccggccagg
     5461 cgataatatc cagtcgccct tccggtttat ctaaattggt aggtggttcg gcggcgtgag
     5521 cggtcattat tgtcatggtg agcgcacaca ggctgctgcg ggcaaatgtc ttgctcataa
     5581 ggtcttactc ctgtcgtaat taattgttca gccgccgtgc cgtaaaaata tctccttgtt
     5641 taaccataga cggcggcttg caggcacacc ttcgcccctg agaaaattta atcaggttgt
     5701 gagctaccgc gcattatgcc gtctgtaagg ttatcttctg gagtatagac aaggggttag
     5761 gaaatatgac tgctattcga aattcatatt acccaatcta agaggaatta aattaaacgc
     5821 tattacagcc gttcttgaat aagtttgcct aattgtttta ccgcctgttc ttcacgttct
     5881 ccccactgcc acgcggtatt aaaacgaaaa aaacgtgacc agttttcacc ggtagaaaac
     5941 attttacccg gcgcaatact gatatgatgc gtcagtgccg ccaggcttaa ttcgccggca
     6001 tctaacggct cggggagctc cagccagaga aaataaccgc tgtcactatg atgaatttta
     6061 acttccgcag gcagataacg cagcagtgcc tgccaggcac gttgtttacg ttccgcaagc
     6121 tggcgacgca ggcgacgaag atgggcgtcg tatcggcgcg tggaaaggta atccaccagc
     6181 gcaagctgca tcggtgagct ggtggaaagc gtactcatca actgcaagcg ttgaatttta
     6241 cgtgcatgtt ttccggcggc gacccaacca atacgaaaac caggcaccag acatttcgaa
     6301 aacgaagagc aatgcaaaac gccatcgtgg cgatcccacg ctttcgcagg cagcggtttt
     6361 tcccgtccaa aataaagttc gctgtaaacg tcatcttcaa tcagcgttac gttgtactga
     6421 ttgagtaacg ccaccagttg tgcttttttt gcggcgttaa ggtaaatccg agtggattct
     6481 ggctattagt catcagccag cacgctttca ccggatactc ctgcaacgcc agttccaacg
     6541 cctgaagatc tatcccttct ttaacatccg tcgccaccga taacgctttc agccgtagcc
     6601 gttccagcgc ctgcaacgcg ccatagaaac aaggattctc tactatcacc caatcgcccg
     6661 gttcagttac cgcttgcaaa ctgaggttta atgcctctaa cgccccggca gtaatgacaa
     6721 tttcatcagg agaaatggtg atgccctgta aggcatagcg acgagcaata gcctgacgca
     6781 gttctgcgtt tcctggcggt aagttttcaa tcacgctcat cgccgtggcg gttttgctta
     6841 cctgcgccag cgagcggttt agttgttgga gggggaaaag tcgcgggtcg ggaaaggccg
     6901 aggcaaacgg aacgaccgac ggatcgcgac tggcctgcaa catatcaaaa atataagtgt
     6961 tgatatcgac tgcttcatct cgagtgactg gaatgactgg cgctttcggc atttttattg
     7021 cctgtggcgc aacgtaataa cccgactgcg gtcgtgcgat aatatatccc tgactttcga
     7081 gcaactgata ggcatggctg acagtcataa agctcatgcc tgaaagcgcc acctggtcac
     7141 gcaacgaagg caaacgatcg ccgggttgcc agatacccga cgcaatctgc tcgcgtaatt
     7201 gttctgcaag ctgctggtat tttttcatta tcgaatcgta attatgtgca gatgattcgg
     7261 cagtctatat cagttgttaa aaatgacaac ttttcgttaa ctgtaattaa accatcacca
     7321 gcgataactc tttgccaagc gcctttgccg caagctggac ggcgtcgatt tttgtcgcat
     7381 ggtgcaagtt aaataggcga gtaatctcct gtttaggttt gccaattcgc ctggctaact
     7441 cttgctgagt gatttctgac tgtaaaaaag catttaacag caatacttta gaggcgacgc
     7501 tcaaaggtac ttcaataaag tgatcatgac tatttaatgg cgaaggtaac gggataagct
     7561 cgttatcttc aaaataaaaa tcaaatgcgg tcagtaaagc atcttttgcc gcttccatcg
     7621 cttcagcgac agtttcgccc tgcgtcaacg cttcagggat atccacaaaa gaaaccatat
     7681 aaccgccttc cggcgcgggt gtaagagtga cgggataacg cataatttcg actgtccctc
     7741 gcatgtcatt aaccttttta attggcgatt aactcaaacc gagttgtttc aggattgctt
     7801 tacgcaatgg ttctttaatc tcatcgcagg ggtgacgcgg catgacactg cgcctcccat
     7861 gaaacctgag tttcaaatgg ttgctgccat tcgctacatc gacgccctga gattcgagcc
     7921 aacgtctgaa ctcgctttgt ttcacatcgc ctccctgccg ctgagtatat caacaaagta
     7981 aacattagtg tttacctccg tgtgtaattt gctcgaaaat agcactaaat cgccgcaacg
     8041 gcagaatgcc atgcaaaatc aggaagaaag actccagaat ttctttgcgc taatttgcaa
     8101 aactgaataa taacctcaag aaacgatcgg catcatagcg gcaaaaggta agaaaaaggt
     8161 ggcaggatgt ttacgaaggc gttatcggtt gtcttattaa cgtgtgcgct gttctcagga
     8221 caactcatgg cagggcacaa aggacatgaa tttgtgtggg taaagaatgt ggatcatcag
     8281 ctgcgtcatg aagcggacag cgatgaattg cgtgctgtgg cggaagagtc ggcggaaggt
     8341 ttgcgcgagc atttttactg gcaaaaatcg cgcaaaccag aagcgggaca acgttgagtc
     8401 atttgttgcc ggatgcggcg agacgcctta cccggcaggc aatatcatgc agattcaatg
     8461 agttgtgagt aaccagaaaa ctggcgtttt acccttttcg cttaggcaat gttttcagca
     8521 gctcatccgg acttacggat gccactacgc ttcccggtaa cggcattttc agtatgtgat
     8581 ttttgatttt gccaatgacg tgcatctcgc atgggcggca atcaaacttt agcgttaata
     8641 cttcatcgcc gttaaccagt tgcattggcg tcgccttcca gcttttgata ttgcctttcg
     8701 cctgtttcgg gcacagatta aaggcaaagc gcagacaatg cttggtgatc atcaccggga
     8761 cttcgccctt ctcttcatgt gcttcatacg ccgcgtcaat cagctgcaca ccatagcgat
     8821 gataaaattc acgcgctttc tggttgtata cgttcgcgaa gaaactcaga tgcgtttgcg
     8881 gataaaccgg cgcaggatca gcaaccggtt tacggctgcc gcgctggtaa ctggcaagac
     8941 gcgcagcatc cagcatgtca gcagcttcac ggcggaactg gtttaacaga ctgtttggta
     9001 caaacagcgc ccccggcaaa ttaatttgca catcgcgggc gtaatagagg gtttgcccca
     9061 gttttgccag accatccttc agattgttca ttgctttttc ggcgttattg gcttcgtcga
     9121 actgcccgtc cagcgtatgc gtgatgctga caccctcttc actggtgagg gtcagaatca
     9181 gttgttcctg ccagccgccc agttcaatgt ctaccgccac ccgacgttcg ctggaggttt
     9241 ttgtcagtgc ctgctgccag ttatgatcaa gattacggtt tagtgggtga tgtggacgaa
     9301 ttttgtgcaa atctgctggc atttcattgg gccagacgcg gtactgattt tctccggttt
     9361 tctcgaccgt attggcacga aaaccgacga cttcacgttt aatcatcacg ttcaggccat
     9421 caccatttgc cagtggctcg gtgacggcaa catcgagatg atctttcgcc actttcaata
     9481 cttcgcctac cggcaggccg ataaatttcg gcgaatcgaa cgcgccaata tcgcctttac
     9541 gggcattcac aaaataatct gtgctaccac ggtggaaagt cttttccgtc gatggaacaa
     9601 agaaatgttc agtacgacct gatgaagcgc gcgccagatc gccacgttct tcaataatgg
     9661 catcaagcat ctggcgataa tgggcggtga tattcttcac gtagctcata tctttgtaac
     9721 gcccttcaat cttgaaggag cgtacgccag catcaatcag cgcgccgagg ttggcagtct
     9781 gatcgttatc tttcatcgac agcagatgtt tttcatagga aaccacccgc ccctgatcgt
     9841 ctttcaatgt gtatggcaaa cggcacgcct gcgagcaatc accacggttg gcgctacgcc
     9901 ctgtttgcgc atgagaaatg tagcactgac ccgaataggc cacgcacagt gccccatgaa
     9961 taaagaattc aatggtcgcg tccgtggcct ggtgaatcgc gcggatctgc tcaagattca
    10021 gctctcgcgc cagcacaatc tgcgtgaagc caacatcaga gaggaacttc gctttttcta
    10081 ctgtacgaat gtcgcactgc gtactggcgt gcagttcaat cggcggaata tcaagttcca
    10141 gaatccccat atcctgaaca atcagcgcat cgacaccggt ctggtagagg tcggtaatca
    10201 gccgttgcgc gggttccagc tcatcatcat gcaaaatggt gttaagcgtg acgaaaattt
    10261 ttgcaccata acgatgggca aacggcacca gctcggcaat atctttcagg ctattactgg
    10321 cattatgacg ggcaccaaaa ccagggccgc cgatataaac agcatcggca ccgtgcaaaa
    10381 tagcttcgcg ggcaatggcg gcatcgcgtg ccgggcttaa cagttcaagt cgatgagaag
    10441 atacggtcac attcttatcc agtagctgaa aaatggcggc tattttagcc acaagcgcga
    10501 cgttgcgaaa tagttttccc gcttaactgc gcgggtaatg gatgagggaa tgaaaatgta
    10561 cggtttgctc cccaccatta cgatagatat gcgtgacgtc tgcagcaaat cgaacgcctt
    10621 ccccacaatt aagggtctgc cattccccat caacgcacag atcaagttgt ccatcaatga
    10681 cgacgacatg ttcgattacc cccttctcat ggggcgtcga ttcgcttatt gcaccagatg
    10741 ccatctgaat cgagaagtgt tcgaagcaga gttgtggatc ataaggaaac agcgaagtga
    10801 tcaccatcgc ctgctgttgt ggatcataaa ctgaaggcgt agcggactgc ggcggagaaa
    10861 taaatgttga aaatggtacg ttcaacccgg tggcaatttt ccataatgtc gcgaccgtcg
    10921 gactggactc attacgctca atctgcccta acattgcttt agaaacgccc gtcgcttctg
    10981 ccagtcgcga aagactccag ccgcgctgct ggcgtagttg tttaagtgtg gtggataaaa
    11041 agcgagcgag attttccatg cgactcctcc ggcaaaacgg aagtttatca cttgtgcgtt
    11101 ataacggaca aatgctacgg tgcctgtacg ctataacgca cgaggtgact atgcgtctgt
    11161 tttctattcc tccacccacg ctactggcgg ggtttctggc ggtattaatt ggctacgcca
    11221 gttcagcggc aataatctgg caagcagcga ttgtcgccgg agccaccact gcacaaatct
    11281 ctggctggat gacggcgctg gggctggcaa tgggcgtcag tacgctgact ctgacattat
    11341 ggtatcgcgt acctgttctc accgcatggt caacgcctgg cgcggctttg ttggtcaccg
    11401 gattgcaggg actaacactt aacgaagcca tcggcgtttt tattgtcacc aacgcgttaa
    11461 tagtcctctg cggcataacg ggactctttg ctcgtctgat gcgcattatt ccgcactcgc
    11521 ttgcggcggc aatgcttgcc gggattttat tacgctttgg tttacaggcg tttgccagtc
    11581 tggacggtca atttacgttg tgtggaagta tgttgctggt atggctggca accaaggccg
    11641 ttgcgccgcg ctatgcggta attgccgcga tgattattgg gatcgtgatc gttatcgcgc
    11701 aaggtgacgt tgtcacaact gatgttgtct ttaaacccgt tctccccact tatattaccc
    11761 ctgatttttc gtttgctcac agcctgagcg ttgcactccc cctttttctg gtgacgatgg
    11821 catcgcaaaa cgcaccgggt atcgcagcaa tgaaagcagc cgggtattcg gctcctgttt
    11881 cgccattaat tgtatttact ggattgctgg cactggtttt ttcccctttc ggcgtttatt
    11941 ccgtcggtat tgcggcaatc accgcggcta tttgccaaag cccggaagcg catccggata
    12001 aagatcaacg ttggctggca gctgccgttg caggcatttt ttatttgatc gcaggtctgt
    12061 ttggtagtgc tattaccggg atgatggctg ccctgcccgt aagttggatc cagatgctgg
    12121 caggtctggc gctgttaagt accattagcg gcagtttgta tcaggcgctg cataatgagc
    12181 gtgagcgcga cgcggcggtg gtggcatttc tggtaacggc aagtggattg acgctggtcg
    12241 ggattggttc tgcgttctgg ggattaattg ccggaggcgt ttgttacgtg gtgttgaatt
    12301 taatcgctga cagaaaccga tattgacatc ctccacgccc tgaaggacga ggatccctgc
    12361 tacgttcagg ctgtcgcctg aatcatttcg gtgggttcct gcttcaacgg gcggcctgac
    12421 tgcaccatct ctccacaggc aagaacggcg tgccccgccg ctaaaatgtt acgagcgcca
    12481 tttacatcgg cgttcgctgt atatccacat acctggcatc tgaatttact ttgtgacagg
    12541 cg
//
LOCUS       DAZFWL020000132        12518 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFWL020000132 DAZFWL020000000
VERSION     DAZFWL020000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.132"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1892)
                     /locus_tag="ACHKNP_RS19755"
                     /old_locus_tag="pgaptmp_003951"
     CDS             complement(<1..1892)
                     /locus_tag="ACHKNP_RS19755"
                     /old_locus_tag="pgaptmp_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4706033.1"
                     /translation="MSKVFICAAIPDELATREEGSVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKACTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGH"
     gene            complement(1986..2177)
                     /gene="ydfD"
                     /locus_tag="ACHKNP_RS19760"
                     /old_locus_tag="pgaptmp_003952"
     CDS             complement(1986..2177)
                     /gene="ydfD"
                     /locus_tag="ACHKNP_RS19760"
                     /old_locus_tag="pgaptmp_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HGU4706034.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(2174..2362)
                     /gene="dicB"
                     /locus_tag="ACHKNP_RS19765"
                     /old_locus_tag="pgaptmp_003953"
                     /gene_synonym="ftsT"
     CDS             complement(2174..2362)
                     /gene="dicB"
                     /locus_tag="ACHKNP_RS19765"
                     /old_locus_tag="pgaptmp_003953"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4706035.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            complement(3323..3424)
                     /gene="ydfB"
                     /locus_tag="ACHKNP_RS19770"
                     /old_locus_tag="pgaptmp_003954"
                     /pseudo
     CDS             complement(3323..3424)
                     /gene="ydfB"
                     /locus_tag="ACHKNP_RS19770"
                     /old_locus_tag="pgaptmp_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
     gene            complement(3426..3581)
                     /gene="ydfA"
                     /locus_tag="ACHKNP_RS19775"
                     /old_locus_tag="pgaptmp_003955"
     CDS             complement(3426..3581)
                     /gene="ydfA"
                     /locus_tag="ACHKNP_RS19775"
                     /old_locus_tag="pgaptmp_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4706036.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(3774..4181)
                     /locus_tag="ACHKNP_RS19780"
                     /old_locus_tag="pgaptmp_003956"
     CDS             complement(3774..4181)
                     /locus_tag="ACHKNP_RS19780"
                     /old_locus_tag="pgaptmp_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4706037.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            4259..4486
                     /locus_tag="ACHKNP_RS19785"
                     /old_locus_tag="pgaptmp_003957"
     CDS             4259..4486
                     /locus_tag="ACHKNP_RS19785"
                     /old_locus_tag="pgaptmp_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU4706038.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            4470..4991
                     /locus_tag="ACHKNP_RS19790"
                     /old_locus_tag="pgaptmp_003958"
     CDS             4470..4991
                     /locus_tag="ACHKNP_RS19790"
                     /old_locus_tag="pgaptmp_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4706039.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            4972..5937
                     /locus_tag="ACHKNP_RS19795"
                     /old_locus_tag="pgaptmp_003959"
     CDS             4972..5937
                     /locus_tag="ACHKNP_RS19795"
                     /old_locus_tag="pgaptmp_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706040.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGLKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDQQTAEQDFLTRNPDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDQFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            5978..6397
                     /locus_tag="ACHKNP_RS19800"
                     /old_locus_tag="pgaptmp_003960"
     CDS             5978..6397
                     /locus_tag="ACHKNP_RS19800"
                     /old_locus_tag="pgaptmp_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4706041.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYSSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKARRSRLLLTPSCNVPFEPF"
     gene            6452..7549
                     /locus_tag="ACHKNP_RS19805"
                     /old_locus_tag="pgaptmp_003961"
                     /pseudo
     CDS             6452..7549
                     /locus_tag="ACHKNP_RS19805"
                     /old_locus_tag="pgaptmp_003961"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1202-like element ISEsa1 family transposase"
     gene            7622..7771
                     /gene="ydfW"
                     /locus_tag="ACHKNP_RS19810"
                     /old_locus_tag="pgaptmp_003962"
     CDS             7622..7771
                     /gene="ydfW"
                     /locus_tag="ACHKNP_RS19810"
                     /old_locus_tag="pgaptmp_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfW"
                     /protein_id="HGU4706042.1"
                     /translation="MDNARIDLRSKYYVKSKADHPWLTRRTQSHQQVKPPKLPKKKPD
                     PDKID"
     gene            complement(8208..8540)
                     /gene="flxA"
                     /locus_tag="ACHKNP_RS19815"
                     /old_locus_tag="pgaptmp_003963"
     CDS             complement(8208..8540)
                     /gene="flxA"
                     /locus_tag="ACHKNP_RS19815"
                     /old_locus_tag="pgaptmp_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HGU4706043.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLRAQLEQLLRQQAEKKNKDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(8743..9048)
                     /gene="ydfV"
                     /locus_tag="ACHKNP_RS19820"
                     /old_locus_tag="pgaptmp_003964"
     CDS             complement(8743..9048)
                     /gene="ydfV"
                     /locus_tag="ACHKNP_RS19820"
                     /old_locus_tag="pgaptmp_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HGU4706044.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            9073..9312
                     /gene="relB"
                     /locus_tag="ACHKNP_RS19825"
                     /old_locus_tag="pgaptmp_003965"
     CDS             9073..9312
                     /gene="relB"
                     /locus_tag="ACHKNP_RS19825"
                     /old_locus_tag="pgaptmp_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HGU4706045.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            9312..9599
                     /gene="relE"
                     /locus_tag="ACHKNP_RS19830"
                     /old_locus_tag="pgaptmp_003966"
     CDS             9312..9599
                     /gene="relE"
                     /locus_tag="ACHKNP_RS19830"
                     /old_locus_tag="pgaptmp_003966"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HGU4706046.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            9671..9826
                     /gene="hokD"
                     /locus_tag="ACHKNP_RS19835"
                     /old_locus_tag="pgaptmp_003967"
     CDS             9671..9826
                     /gene="hokD"
                     /locus_tag="ACHKNP_RS19835"
                     /old_locus_tag="pgaptmp_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU4706047.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            10043..10294
                     /gene="rem"
                     /locus_tag="ACHKNP_RS19840"
                     /old_locus_tag="pgaptmp_003968"
     CDS             10043..10294
                     /gene="rem"
                     /locus_tag="ACHKNP_RS19840"
                     /old_locus_tag="pgaptmp_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HGU4706048.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            10361..10639
                     /locus_tag="ACHKNP_RS19845"
                     /old_locus_tag="pgaptmp_003969"
     CDS             10361..10639
                     /locus_tag="ACHKNP_RS19845"
                     /old_locus_tag="pgaptmp_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706049.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            10641..11690
                     /gene="ydfU"
                     /locus_tag="ACHKNP_RS19850"
                     /old_locus_tag="pgaptmp_003970"
     CDS             10641..11690
                     /gene="ydfU"
                     /locus_tag="ACHKNP_RS19850"
                     /old_locus_tag="pgaptmp_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4706050.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            11704..12456
                     /gene="ydfT"
                     /locus_tag="ACHKNP_RS19855"
                     /old_locus_tag="pgaptmp_003971"
     CDS             11704..12456
                     /gene="ydfT"
                     /locus_tag="ACHKNP_RS19855"
                     /old_locus_tag="pgaptmp_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4706051.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
BASE COUNT         3209 a         2783 c         3130 g         3396 t
ORIGIN      
        1 tgatgaccgt tcaccttaat tgggtgatct gccgtctcgc cagtttcatt atcgctattg
       61 tgatgctgat tgccgccagt gttctccttg tgtggatgtt cagcgccttc catttcctcc
      121 ggatcatctt cctgaacttc aacctgatac tcttcatcga atgtttcttg gtatgttgcg
      181 tcgcccatca ccgcgccaca atcagggcag ttgccgccgc cggtctgacc gcaggcggtg
      241 caggcttttt ccacttcctg ttgcgccact ggttcaggct gtttcgtttc tggctcgttt
      301 tgtaacgcat ttggactgtt ttgttccgct ttttggtagt tccgttccga ttcatgctgg
      361 ttctggttca cagaatcgcg ggtctggatc cccttaaccc atttcggatc attcgggtca
      421 ctaatccctt caacaaattc accacgtgat gcagcaagca acttatcggc gtcaggctgg
      481 ctgatattgg ctgcctgcat aattttgttt acttcgtcag cggtaacttt tatcggctct
      541 ggttgttctg aatcttcagc ggtatctaca ttttgcggta agcccgtgta tgtgccattt
      601 tttcgggcaa aatattcttc ttttgtgatt tcagtggcgc cagcagccag tgccttatcc
      661 agaccagaaa gtttgtttgc gcgaccgtat ttttctccgt ccttatctgc gaagaggaaa
      721 tagaacggcc cctcacgctc tacagatggt tcagcttccg gcgcggtttc attttttggg
      781 atatcagata cctcagtttc cactgcatca gtttgtgttt ctgatgactg gagaacatca
      841 acagtgccca ggtctgtttc ttcattctca aacacgccct ttgtcgtcag gtattcgcag
      901 atatatttgt tcagtgctac gggatctttg tgaatgtcga tcggacgctc acggacaagg
      961 ccaaaaatag tttggcggtc gtagcgaagg gcatcaggct gtttgcgcat tgatgccgag
     1021 atacgcttcc agtcttcgcg gtcgttgtcg ataacttctt tttttgccca gcgatggatg
     1081 ctgccgtcaa tgtttccggc atccacatca ccaggccaga gagcgtaggc cagttcgtca
     1141 tccagtgttt tccatgtctg cttgtattcg cgatgaatgg cagcaatgac cgggctgatt
     1201 tttcctgttg aattttcagt gtactgttga ttggctctgg cgcgggcgag atcaacaaca
     1261 gacgtgtatt ttccggtttc cttgcgttca ccttcgcgac gttttttcca gatgcgcatc
     1321 tctgcctgaa tttcgggcca tttagcacca ggaatacatt tatgcttaac ccacccaatg
     1381 gcgtgcaact taagctccgg atacatggcg ttaacttctg gcattttcat caacgcttca
     1441 acgatatgtc cgtcgaatgt tgccatgtct tcctgcaaca attcctgtgc gctaataacc
     1501 atatcaacgg tgatgttttc acatgtgtcg aacttaacca tgacagcgtt ctgtacttca
     1561 ggggccagct tgtcaaaagt gacgttcatc ggatcggatt cagtctcaac cgggacaaaa
     1621 gaagcagact cctcatccca gcggttttcc tgcatatatt cagcatccca tgaatcgagg
     1681 gcagggcggg gtatgccagg tttatcctcg cagacaataa atttataagc gcagtcctga
     1741 gcagccggat aatgttccag gaactgccag tgaaattttg ctcgagcacg gcgttcgtcg
     1801 ccagcttcaa tggctgtggc tacagccaca gagccttctt cccttgttgc cagttcgtca
     1861 ggaatagcgg cgcaaataaa gactttactc attttgtttt aacctcatga cagatttaag
     1921 gatgaacaaa tccctgccat tgctggcata taagaatgaa accggatatt tattacggaa
     1981 ctgttttaaa gacctgccgg gatttcgata ttatcctggt gaataacttt atcgaccggg
     2041 taacagttac cgggaatttt ctgttcggtt gctgcagtca tacactcctg cattgtcctg
     2101 tgaacactga ctgcaatatc aactggcact ccggaaacaa gaaaaactgt cagaacaagc
     2161 gcaaatgctg aattcattgt gcacatcctt ttggcatcag acgtaaacga gccagcattg
     2221 aaacaatgca tattttattt aatagctccc gttcttgttt tctcttgtta atggcatctt
     2281 cagtaaatac agggttactg atagtgacac caatttcaaa acaaccttca gacgtattaa
     2341 cgtttggtaa taacgttttc attatcgcgt cctcaacaat gaattttgtg atgcagtgcc
     2401 tggtgcctcc aggtgacgtt aaccagttaa caattaacgt cggatacaga gaatccaccc
     2461 ataacactgt ttttggtttt aactgttccg cgtgcgctta gccgtattca ccgcatcaca
     2521 aaattcactt taaaaagggc ggcagagcag tcacggagta aaactgatac cgccaaacgt
     2581 caccagaaaa ttgataacag agggcgttgc agcggggttg tcacttaagc gtatggtcaa
     2641 cctgacaacc cggtgtcctc aacggggaag gaataacccc gccatactta ccgccgcgcc
     2701 attttgcggg ttgccacaac cggaagcgca cggtcgaatt aaatttaacg acaccgtaca
     2761 gtgagacgaa cttcgccgtg cgctttcgtg ttgtgtgcct gcttttaacc acgtcaggcg
     2821 aggtggtatc cttaaaatca ccacagtttt aaggattcat taagcaatgt cgcaaccacc
     2881 aataaatccg cttaagaaca tgaaaattga ttactggtat aaagcgctta cagttgttgg
     2941 cgctgcgttg tttgtcttta atggaacgtc tttttttgac agatatcccg ttgttccatt
     3001 gggttttttg tcctccggca tcttttttat tggtttgggg gagtggatta atcaccctct
     3061 catagtgaga tttattggtc ctggagtttg gactcgtgga tataatcgtt cttcgtgcgc
     3121 actcggtatc atcttcgaca tacttggttg tttcctgatt gttacaggag tcgtcaagtt
     3181 cttctgatgt aaaaccgcaa atggggcacg taacgggaat tttgaaaagc gtttctccgg
     3241 gttccagaac aaaattttct gcggtctgat tttgcttctc atatttgtgc tccgcgtcat
     3301 tgtgagagca cattcttatt ctgagtgcct gtttaaactc actgaagctg agagcttctt
     3361 cgccttcggc aaggccttcg aagtattctt cgtaagcctt ttccatgatt gtgttgaaat
     3421 ccatatcact cacctgagtt tctttccagc cagcgacggg caccattttc ggttttaaac
     3481 gttttgcttt tggtatacgt catcgcggtg aacgtgccgt cctggttggg gaacacgcca
     3541 cataccagag attcgctgtt gccaagatcg atagtatcca tgctgacctc atttcccctt
     3601 aacgccgggg tagcggaact gtttgctgag aacaccgtgc ggtgtcttga tggaggtaat
     3661 ttagttttct cattattttt cgtcaaggtt ttttgatgag aaaactcaag cgttggcgca
     3721 agataaagcc aatacattga aatgtaaggc tttaaaattt tgtgaaggga tgattattga
     3781 tgcttgttgc gtttgcgagc ctctaatagc tcggtgaaca ggcgattaaa attctcaacg
     3841 cgggcgcgga gttcgctgag ttgtgcttgt tgctctgatt taggaagtgc gcgatacaaa
     3901 cgcagcatct ctaactcatc ttccgataag tctaaggcac tatcgagtga aacaggggga
     3961 gctggtgttt tgtcctcgtc gccaaacagt atccaagttg gtgaacattg caatacctcg
     4021 gcgaggcgat gcaaattttg cccgcgcggg gctgtatggt cactttccca taatgaaatt
     4081 gatgagccag atacgccagc agctttgctt aaaccgtttt gacttaagcc tacctgctta
     4141 cgtctttctc taatgcgttg acctaaagtt ttctcgttca tatttagata tcttaataac
     4201 ccttgacttg agattccttg agtgattagc attgagaaaa ctcaatattg gaggtgcgat
     4261 gtttaaatca gacgtaatta atttttacgg tacgaaagcc aaagtagcga aagctgctgg
     4321 cgttgaccca tctgctgttt ctcaatggca agagctggtt cctgaaggtc gcgcgatgcg
     4381 tctacaggag gcatctggcg gcgagcttct gtatgatccc aaggtttatg acgaatatcg
     4441 taagacgaag cgggcggggc ggttgaacaa tgaaaatcac tcctgaacag gctcgtgagg
     4501 ctctggatgc ctggatatgt cgaccaggaa tgacacagga gcaggcgacg atattaatca
     4561 ctgaagcatt ctgggctttg aaagagcgcc cgaacatcga tgttcagcgt gtcacagatg
     4621 aaggtggcgc ggttgatcag cgagcacttg gcgttaatcg agtgaagata ttcgaacgct
     4681 ggaaggctat cgacaccagg gataagcgtg aaaagttcac ggcgctagtg cctgcaatta
     4741 tggaggctat tcggattaat gatttcaggt tgtatcgtga aattagtgac ggaaaaagca
     4801 tcacgtacat gatcgccggg ttaaacaaag aatatggcga tgtggtggag tccggactgc
     4861 tttttgctga tcctgccgta gtggatcgtg aaactgacga acttatagaa aaagcaattg
     4921 ctttcaaact tgcgtatcga cagcaatacc aacaaaaagc tggatggaat tatgagcctt
     4981 ctttttgctg aacgcccact ggttataaac acacagcttg cgatgaagat tggcttaaat
     5041 gaagccattg tgttgcagca gttgcattac tggttgagag ataccaattc cggcatggaa
     5101 tgtgatggtg ttcgctggat ttacaacaca acggaacaat ggctggaaca gttcccattc
     5161 tggtcagagt caacgttaaa gcgcgcgttt gcaagtctga aaacgctggg gcttttgcgt
     5221 tgtgaaaagc tcaataaatc aaagcgcgat atgaccaatt tctacacgat caactacggg
     5281 agcgagcttt tagatggtgg caaattgagc gaatccatcg gtttaaaatg cgccgctcca
     5341 tcaggtcaaa atgacacgat ggaagaggtc aaaatgaaac gctccattgg ttcaaaacga
     5401 cccaatgtca tcgggtcaaa atggcctgat gatcttacag agaatacaac agagattact
     5461 acagagaata aaaacacttt tcgtccggaa gcttcgcaac cggaccagca gaccgctgaa
     5521 caggattttt taacccggaa ccccgacgcg gttgtgttta gtgcgaaaaa acgccagtgg
     5581 ggtagcaggg aggatttggc gtgtgcgcag tggatttggg ggcggatcgt gaacctttac
     5641 gaacaggctg ccagcgacga tggagagatc atgcgaccaa aagagcctaa ctggacagcc
     5701 tgggccaatg acgtgcgcac aatgcggatg ctggatggca gaagccacag acaaatttgc
     5761 gaaatgtttg gtcgggtaca gcgagatcaa ttctgggtaa aaaacatcat gagcccgtca
     5821 aaactccgcg aaaaatggga cgaactggtc atccgcctgg gacgttcacc tgtacagcgt
     5881 tgtgtgaatc atatttctga accggataca gaaattccgc ctggtttcag gggataagtg
     5941 ttgattttag ggtataaagt aatgttcagg aggacttgtg gcaaaagttt ttacacaaga
     6001 agagcgggaa aaaattaaag ggcaggtggt ggaactcgta cgccagagtg ggcgcgagac
     6061 gttacgacaa ctggaagcta aaactggggc aacaagatat ctgatgagcg ttctggccag
     6121 agagctggtt gccagtggcg atgtatacag ctctggttac gggttattcc cgtctgaaca
     6181 ggcgcgtaag gactggcaaa acgcccgcaa aaaactctcg agggcaaagg tgaagaaacc
     6241 tgctgtggtt gatccggacc ttatctggtc attacctgac ggagaaatac gtcgctacga
     6301 cagtcgtcta aacataatct gtcgcgagtg ccggaaggca aggagaagca ggctattgtt
     6361 aacaccaagc tgtaatgtcc cctttgaacc attctaaaat gtccccagac aattctctgg
     6421 gggatttttc atgatcaaag agactgttac gatgagtcat aaggaactcc accgacttca
     6481 gattattcag gagtcaatta atcgacatat tactcaggaa caagctgcgg cacgcattgg
     6541 catttctatt cggcaggtta aacgtctggt gcaacggtat agaaatgaag ggccttctgg
     6601 tctggtttcc caccgacgtg gaaagcgtcc taataattcc ttttctactg aattcagagc
     6661 aacagtaatt tcactcctca aaggccgtta cgctgatttt ggacctacgt ttgcgtgcga
     6721 aaaattgcgc gagatacacg gtttatcttt atccgttgaa actctcagaa agtggatgat
     6781 agaagagggg ttatggcgtg aacgccgtcg taaaattgcc cgtatatatc aacgccgcat
     6841 gcgacgacca tcttacggtg aactgatcca gattgatggc tcacctcatg actggtttga
     6901 aaatcgaggc cccagatgta cactgatcgt tttcattgat gatgccacca gtgcgttgat
     6961 ggcgttgcgt tttgtgcctg ctgaaacaac ccgggcttac atggaaaccc tccggggtta
     7021 ccttaatgat catggcgtac cgctcgctct ctactctgat agacacagta tattcagggt
     7081 aaataaccca gagcgggaag gtgagctgac ccagttcact cgtgcgataa agacactggg
     7141 catcgagcca atccatgcca acagcccgca ggcaaaaggg cgggtagagc gcgccaatca
     7201 gacactacag gacaggctgg tcaaagaaat gcggcttcag aatatcagtg atattgaaac
     7261 agcaaatgca tggttgccga cctttattga agcctataac aaccggttcg ctacgtcgcc
     7321 tcgtactact gataatgctc atcttgatgt gcaccattct gaagaggaac tgggttatat
     7381 cttcagccta caggcgaagc gcgttctgtc taaaaatctc actttccagt acaaaagcag
     7441 tgcgtttcag gtacgcagtg agggccgggg atatcgactt aggcattcgg ttgttactgt
     7501 atgtgagaac tttgacggtg aaattaacgt tctgtatgac gggaaagcgc tgggctggga
     7561 aaagtatgtt gatggcccgg agcctatacc actggatgat gaaaagagtg tccatgaacg
     7621 agtggataat gcccgtattg atttacgctc aaaatactat gttaaatcta aagctgacca
     7681 tccctggctt acgcgccgaa cgcaaagtca tcagcaagtt aagcccccga agttacctaa
     7741 aaagaagcct gatcccgata aaatagattg aaaccaagat cgattcggtt gagtgcatat
     7801 ccattcatag ggtagattct taagtcgcgt ttctgatgtt cattttcggg tggtttgtta
     7861 cttgttttac cggggatatg ccagaaacgc gctgagtcag tctgggcggt gcgcgtaatg
     7921 aggcgttatg gtaaatagcc tatgctaatg tccgctaaga gcaagaagcg gaagttggca
     7981 gttttgtgga ctgtccccac aaaagtgact acagaaatag ttgcaattca taattgatca
     8041 tgggttgtca gttaaactcg tggcgattta aatagactaa ttgggagtgc gtccattact
     8101 tatatcttgt aatgttaact atcagaaatg atacaaagat aatatgtctt taaagaaaag
     8161 gctgatggcg aaaagtggcc cgatgagggc cacaatacgg ctgtcactta gacgtaaata
     8221 tcaatggtgc cagcggtatt tgtatcgtct tttttctctt cttttttatc aggctgaact
     8281 gtcgcgtctt tattcttttt ctctgcctgc tgccttaaca actgctccag ttgagccctg
     8341 aggctttcaa tttgcttctg taccaatgca gccatttctt ttttctgctg tgtcgtcatc
     8401 ccctcttccg atgagatttt cccaagcttt tcagtcagca cctgaatttg tcttgtgatt
     8461 ttggctattt ctgatgttcc ttccggggcg gagttgtttg aaataacggt tgaggtattt
     8521 ccctgaattg tgacagacat agatttctcc ttttaaaaaa gcactatcgg catgcacaaa
     8581 aaaatcttta atcgtatttc ttgtgtcatt aattgtttga tgttcagatt gttttcctcg
     8641 cgggctggcg cgcctcagaa agtaaagctt gttgacaggg gtaaacgttc ggcaataatt
     8701 ttctgccgca tgcgggtgtt gcataaaacg tgctacgttc ctttatcgac aggtcaggtc
     8761 accgctcacc cgccgacgag aaagcaacac tgacatgcta aagcaaaaaa tagatgaata
     8821 agttgagttg tgcatatgta gcctgaccgt cacaaagtat atggtgtctg taccagtaag
     8881 atgatggccg gactctttaa aaacgagctg acctgcacaa tacaggatgg acttagcaat
     8941 ggctgctcct ggcacaaagc ggacagtgat caccgttctt acgactactt tctgacttcc
     9001 ttcgtgactt gccctaagca tgttgtagtg cgatacttgt aatgacattt gtaattacaa
     9061 gaggtgtaag acatgggtag cattaacctg cgtattgacg atgaacttaa agcgcgttct
     9121 tacgccgcgc ttgaaaaaat gggtgtaact ccttctgaag cgcttcgtct catgctcgag
     9181 tatatcgctg acaatgaacg cttgccgttc aaacagacac tcctgagtga tgaagatgct
     9241 gaacttgtgg agatagtgaa agaacggctt cgtaatccta agccagtacg tgtgacgctg
     9301 gatgaactct gatggcgtat tttctggatt ttgacgagcg ggcactaaag gaatggcgaa
     9361 agctgggctc gacggtacgt gaacagttga aaaagaagct ggttgaagta cttgagtcac
     9421 cccggattga agcaaacaag ctccgtggta tgcctgattg ttacaagatt aagctccggt
     9481 cttcaggcta tcgccttgta taccaggtta tagacgagaa agttgtcgtt ttcgtgattt
     9541 ctgttgggaa aagagaacgc tcggaagtat atagcgaggc ggtcaaacgc attctctgaa
     9601 ccaaagcatg acatctctgt ttcgcaccga aggtgacact tctgctttgc gttgacagga
     9661 gaagcaggct atgaagcagc aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaac
     9721 cgtcatagtg acggcactgg taacgaggaa agacctctgc gaggtacgaa tccgaaccgg
     9781 ccagacggag gtcgctgtct tcacagctta cgaacctgag gagtaagaga cccggcgggg
     9841 gagaaatccc tcgccacctc tgatgtggca ggcatcctca acgcacccgc acttaacccg
     9901 cttcggcggg tttttgtttt tattttcaac gcgtttgaag ttctggacgg tgccggaata
     9961 gaatcaaaaa tacttaagta gcgcgcaggg ataagaggga tggtccctta aaggggagag
    10021 ctaattatcc ggaaggattc tgatgatgaa catcgaagaa ctgcgtaaaa ttttttgtga
    10081 agatggcctc tatgctgtgt gcgttgaaaa tggaaatctt gttagtcatt accgcattat
    10141 gtgtttgcga aagaatgggg ctgcgttaat taattttgtg gatgctcggg tcacggacgg
    10201 atttatcttg cgcgaaggtg agtttgtcac ttcattacag gcattgaaag agatcggaat
    10261 aaaagctggc ttttctgctt tttcaggaga ataaactcat ctacaatctt gcgcggggct
    10321 gaactcccgc tgagtaacac cgtgccaccg gagaaaaccg atggcacgca acgcaaaata
    10381 ttacaattct gataattcgc ccgttcttgc ctgcacgcac gggcggtatt ctcacgcatt
    10441 caagtctgaa tggttccagc accctccatg cactgcagaa caggccgaat ggctgattca
    10501 ttcttaccgc aggcgcgggt tcgaggttaa gaaagctctc agtctcgact atcggcactg
    10561 gataatctct gtcaggctgc cttattccga acgcccacca cgtgcgtccc gcactttcca
    10621 gcaacggatc tggaggtaac gtgcgggtat tacttagacc tgttctggtg cctgagcttg
    10681 ggctggtggt ccttaagccg ggccgtgaat ccatacagat atttcataat cctcgagtgc
    10741 tggtggaacc ggaaccaaaa agcatgcgta atctgccatc cggagtcgtt cctgccgttc
    10801 gccagccgct ggcggaagac aaaacattgc tgccgttttt tagtaacgaa cgggtgattc
    10861 gtgctgctgg cggcgttggc gcattgtccg actggctatt acgtcatgtt acatcctgcc
    10921 agtggcctaa tggtgattac catcacactg aaacagtcat tcaccgttat ggtaccggcg
    10981 caatggtgtt gtgctggcac tgcgacaacc aactgcgtga ccagacatcg gaatcactgg
    11041 agctgcttgc tcaacaaaat ctgacagcat gggtgattga cgtcatccgt cacgcaataa
    11101 gcggtacgca ggagcgggaa ttatctttgg ctgaattatc ctggtgggcg gtctgcaatc
    11161 aggtggtgga tgcactacct gaggctgtat cgcgtcgttc gctgggatta ccagcggaaa
    11221 aaatctgctc ggtgtaccgc gaaagcgaca tcgtaccggg agagcagacc gccaccagca
    11281 tattgaaaca acgcacaaaa aatcttgcac cgttgcctta cgcccaccag caacaaaaat
    11341 caccacagga aaagacggtg gtaagcatca ccgttgatcc agagtctccg gaatctttca
    11401 tgaagctgcc taaacgtcgc cgctgggtta aggagaaata cacacgttgg gttaagacac
    11461 agccgtgtgc ttgctgcggt atgccagccg acgatccgca tcatctgatt ggtcacgggc
    11521 agggcggaat gggaacaaaa gcacatgatc tctttgtgtt gcctttgtgc agaaagcatc
    11581 acaacgagct gcatacggat acagtggcat ttgaagataa gtatggctcc caactggagc
    11641 tgatatttcg ttttatcgat cgcgcgctgg caattggcgt actggcgtaa gtggagaacg
    11701 agcatgaacc ttgaagcctt accaaaatat tactccccaa aatctccaaa attgagcgat
    11761 gacgctccag cgacaggcac cggttgttta acaattacgg atgtaatggc agcgcagggg
    11821 atggtgcagt cgaaagcacc acttgggttg gccttatttc tggcaaaagt tggtgttcag
    11881 gaccctcagt ttgcgattga aggcctgcta aattacgcga tggcactgga taacccgaca
    11941 ttgaacaaat tgagtgaaga aatccggtta cagattattc cttacctcgt gaattttgcc
    12001 tttgctgatt actccaggtc tgcggcaagt aaggctcgct gtgagcattg ttcaggtacg
    12061 ggattttata atgtattgcg cgaagtggtg aaacactacc gacgcgggga atctgtaatc
    12121 aaggaagaat gggtgaagga actatgtcag cattgccatg gtaagggtga agtcagcaca
    12181 gcgtgcagag ggtgtaaggg taaagggatt gttctggatg aaaaaagaac ccggtttcat
    12241 ggcgtaccgg tatataagat ttgtgggcgt tgtaatggaa accggtttag tcgtttaccg
    12301 accacgctgg cacgacgtca tgtccagaag ctggtaccag acctgaccga ttatcagtgg
    12361 tataaggggt atgcggacgt cattggtaaa ctggtaacaa agtgctggca ggaagaagca
    12421 tacgcggaag cgcaattgag gaaggtgacg agataaatga tttttgctga agatggcgac
    12481 atgatgtttg catttttcaa aaaatatgga taaaattt
//
LOCUS       DAZFWL020000133        12399 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFWL020000133 DAZFWL020000000
VERSION     DAZFWL020000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.133"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(104..385)
                     /gene="yehE"
                     /locus_tag="ACHKNP_RS19860"
                     /old_locus_tag="pgaptmp_003972"
     CDS             complement(104..385)
                     /gene="yehE"
                     /locus_tag="ACHKNP_RS19860"
                     /old_locus_tag="pgaptmp_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HGU4706052.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(648..1757)
                     /gene="apbC"
                     /locus_tag="ACHKNP_RS19865"
                     /old_locus_tag="pgaptmp_003973"
     CDS             complement(648..1757)
                     /gene="apbC"
                     /locus_tag="ACHKNP_RS19865"
                     /old_locus_tag="pgaptmp_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HGU4706053.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1889..3922
                     /gene="metG"
                     /locus_tag="ACHKNP_RS19870"
                     /old_locus_tag="pgaptmp_003974"
     CDS             1889..3922
                     /gene="metG"
                     /locus_tag="ACHKNP_RS19870"
                     /old_locus_tag="pgaptmp_003974"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGU4706054.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4063..7857
                     /locus_tag="ACHKNP_RS19875"
                     /old_locus_tag="pgaptmp_003975"
                     /pseudo
     CDS             4063..7857
                     /locus_tag="ACHKNP_RS19875"
                     /old_locus_tag="pgaptmp_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7867..11499
                     /gene="yehI"
                     /locus_tag="ACHKNP_RS19880"
                     /old_locus_tag="pgaptmp_003976"
     CDS             7867..11499
                     /gene="yehI"
                     /locus_tag="ACHKNP_RS19880"
                     /old_locus_tag="pgaptmp_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HGU4706055.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVIARQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFNQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVHRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11561..11878
                     /gene="yehK"
                     /locus_tag="ACHKNP_RS19885"
                     /old_locus_tag="pgaptmp_003977"
     CDS             11561..11878
                     /gene="yehK"
                     /locus_tag="ACHKNP_RS19885"
                     /old_locus_tag="pgaptmp_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HGU4706056.1"
                     /translation="MIVQKELVAIYDYEIPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDTDPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         3239 a         3149 c         3046 g         2965 t
ORIGIN      
        1 tgtagattta tgtaaatggt attgttgata tcaataaaaa agccgaaatt atttatataa
       61 atgatttcgg ctttctaaat gcaattaaaa tcaggttgcc aaattaatcg tagcgattta
      121 acacgattcg acgtttgcta tcgctacctg caacaaccac ttctgtcgtt acacctttga
      181 cgggcgaaga aaacagatga cgggtgtcaa cattatacgt taactgacca caatgctgtt
      241 gaggttgacc attaaccata gccatactgc atgttggggg actgatcctg ccattaatcg
      301 ctaatgtggc agtctctgaa gagaatgcgg gagaagccaa tccataggca agaaaaataa
      361 ttcctgacag ccaatactta ttcattgaac gttatccctg tagtaaaggt tatgcctgga
      421 tagaatgagt gcataacaaa ctatagctgt acatccacta cacagccacg aaggatgata
      481 atgaagcatt gcctgtatga tcaatcgact ttgtagagtt tcggacgaag gtccgcagaa
      541 tattcgcagt attaaataag tcttcattaa tgcggcaaaa actaatacac cgcatcaatg
      601 taacatctct gcggtgtatg aagaataacc agaatggtta ataggcgtta gaccgcgcgg
      661 aaggaaatct cgcctggaat gacttcaccc tgccagtaga gctgagctgc aacgcggtca
      721 gccagctggc ggtagatagc ggtaaattcg ctctccggac ggctgataac cgttggcgtg
      781 cctttatcga gatcctcacg taaggagatg tgtaacggca tctgccccag cagctgtgtg
      841 tggtattttt cagccagttt ctcagcacca ccggtaccga aaatcggttc atggtgacca
      901 cagttactgc aaatatgcac actcatgttc tcgacaatac caagcaccgg aacctcgact
      961 ttctcgaaca tcacaatgcc ttttttcgca tcgatcaacg cgatgtcctg cggggtggtg
     1021 accacgactg caccagttac tggaatgttt tgcgccagcg tcaattgaat gtcaccggtg
     1081 cctggcggca tatcaagcac cagatagtcg agatccggcc acaaggtttc ctgcaacatc
     1141 tgcattaacg ccttgctcgc catcggtcca cgccacacca tcgcattgtc gtcagtgacc
     1201 agataaccaa ttgaattggt tgccaggcca tgagacataa tcggtgccat gtgggtaccg
     1261 tccggcgacg ttggacgctg attttctgcg cccagcatgg tgggaattga tgggccgtag
     1321 atatcggcgt ccagaatgcc gactttcgcc ccttccgccg ccagtgccag cgccaggttt
     1381 accgccgtgg aggatttacc cacgccgcct ttaccggaac tgaccgcaat gatgttcttc
     1441 acaccattaa tacccggctg attcttcacg cgttttaagg tggcgatatt gtgcgacagt
     1501 ttccagtcga tagctttcgc cccggtgata cgcagcagtt cggcgctaca ttgctctttt
     1561 aactcttcaa acgcgctatg ccagacgaat ggcataacca gttccacatg caacgtgtcg
     1621 tccatccagg cgacatggtg caacgctttc agcgtggtga ggttatgctt cagggttggg
     1681 tgctgaaaat tggccagtgt cccggcgacc attgcgcgca aggcttctgg tgatttggcc
     1741 tgggattgtt cgttcatccc gactcctttt ttgtatagat aaaccatcag ctgatagttt
     1801 acctgaagaa tatagagaag tacttactta acattttccc atttggtact atctaacccc
     1861 ttttcactat taagaagtaa tgcctactat gactcaagtc gcgaagaaaa ttctggtgac
     1921 gtgcgcactg ccgtacgcta acggctcaat ccacctcggc catatgctgg agcacatcca
     1981 ggctgatgtc tgggtccgtt accagcgaat gcgcggccac gaggtcaact tcatctgcgc
     2041 cgacgatgcc cacggtacac cgatcatgct gaaagctcag cagctcggta tcaccccgga
     2101 gcagatgatt ggcgaaatga gtcaggagca tcagactgat ttcgcaggct ttaacatcag
     2161 ctatgacaac tatcactcga cgcacagcga agagaaccgc cagttgtcag aacttatcta
     2221 ctctcgcctg aaagaaaacg gttttattaa aaaccgcacc atctctcagc tgtacgatcc
     2281 ggaaaaaggc atgttcctgc cggaccgttt tgtgaaaggc acctgcccga aatgtaaatc
     2341 cccggatcaa tacggcgata actgcgaagt ctgcggcgcg acctacagcc cgactgaatt
     2401 gatcgagccg aaatcggtgg tttctggcgc tacgccggta atgcgtgatt ctgaacactt
     2461 cttctttgat ctgccctctt tcagcgaaat gttgcaggca tggacccgca gcggtgcgtt
     2521 gcaggagcag gtggcaaata aaatgcagga gtggtttgaa tccggcctgc aacagtggga
     2581 tatctcccgc gacgcgccat actttggttt tgaaattccg aacgcgccgg gcaaatattt
     2641 ctacgtctgg ctggacgcac cgattggcta catgggttct ttcaagaatc tgtgcgacaa
     2701 gcgcggcgac agcgtaagct tcgatgaata ctggaagaaa gactccaccg ccgagctgta
     2761 ccacttcatc ggtaaagata ttgtttactt ccacagcctg ttctggcctg ccatgctgga
     2821 aggcagcaac ttccgcaagc cgaccaacct gtttgttcat ggctatgtga cggtgaacgg
     2881 cgcaaagatg tccaagtctc gcggcacctt tattaaagcc agcacctggc tgaatcattt
     2941 tgacgctgac agcctgcgtt actactacac tgcgaaactc tcttcgcgca ttgatgatat
     3001 cgatctcaac ctggaggatt tcgttcagcg tgtgaatgcc gatatcgtga acaaagtggt
     3061 taacctggcc tctcgtaatg cgggctttat caacaagcgt tttgacggcg tgctggcaag
     3121 cgaactggct gacccgcagt tgtacaaaac cttcactgat gccgctgaag tgattggtga
     3181 agcgtgggaa agccgcgaat ttggtaaagc agtgcgcgaa atcatggcgc tggctgatct
     3241 ggctaaccgc tatgtcgacg agcaggctcc gtgggtggtg gcaaaacagg aaggccgcga
     3301 tgccgacctg caggcaattt gctcaatggg cataaacctg ttccgcgtgc tgatgactta
     3361 cctgaagccg gtactgccga aactgaccga gcgcgcagaa gcattcctca atacagaact
     3421 gacctgggat ggtatccagc aaccgctgct gggccacaaa gtgaatccgt tcaaagcgct
     3481 gtataaccgc atcgatatga agcaggttga agcactggtg gaagcgtcta aagaagaagt
     3541 gaaagcagct gccgcgccag ttactggccc actggcagat gatccgattc aggaaaccat
     3601 cacctttgac gacttcgcaa aagttgacct gcgcgtggcg ctgatcgaaa acgcagagtt
     3661 tgttgaaggt tctgacaaac tgctgcgcct gacactggat ctcggcggtg aaaaacgcaa
     3721 tgtcttctcc ggtattcgtt ctgcctaccc ggatccgcag gcactgatcg ggcgtcacac
     3781 cattatggtg gccaacctgg caccgcgtaa aatgcgcttt ggtatctccg aaggcatggt
     3841 aatggctgct ggtcctggcg ggaaagatat tttcctgcta agcccggatg ccggtgctaa
     3901 accgggtcat caggtgaaat aatccccctt caaggcgctg catcgacagc gccttttctt
     3961 tataaattcc taaagttgtt ttcttgcgat tttgtctctc tctaacccgc ataaatactg
     4021 gtagcatcag cattcaactg gataaaatta cagggatgca gaatgaaaca ctttatctat
     4081 caggacgaaa aatcacataa attctgggca gttgagcaac agggaaacga gttgcatatc
     4141 agttggggaa aagttggcac caaagggcaa agccagataa aaagtttttc agatgctgcg
     4201 gcagcggcaa aagcggagct taagctgatt gcggagaagg tgaagaaggg gtatgtggag
     4261 caagcgaagg ataattcttt gcaaccttcc caaacggtaa cgggctctct caaggtagcg
     4321 gacttatcca ccattattca ggaacaaccc tctttcgtag cagaaacccg tgcgcctgac
     4381 aaaaatacag atgctgtttt accgtggctg gcgaaagata ttgctgtcgt ttttccgccc
     4441 gaagttgtac acaccacgtt aagtcatcgc cgctttcccg gagttcctgt tcagcaagca
     4501 gacaaattga cccaattacg tcgcttagcc tgtagtgtgt cgcaacggga taataaaaca
     4561 gccacatttg acttcagcgc ctgttcttta gaatggcaaa acaccgtcgc ccaggcgatc
     4621 agtcagatcg acggcctgaa aacaacacag ctaccatcac cagtaatggc tgtactcacg
     4681 gcacttgaaa tgaaatgcac aagatataaa gtgcgtgagg atgttatgga tcagatcgtc
     4741 caggaaggcg gtctggaata tgctactgat gtaataatac accttcaaca gattgatatt
     4801 aaatgggatt atgcgaataa tgtcattatt attctgccgt ctggcattgc acctgactac
     4861 ttggagcaat attccagatt tgaattacgc ctacgtaaac atttatcact ggcggaagag
     4921 tctctctggc aaaaatgtgc acaaaaactt attgccgcaa ttccacatat tccagaatga
     4981 cggcaaccat taattgcttt gttattacct gaaaaaccag aaattgcaca tgaaattgcc
     5041 cagcgtctac tggggcaaaa aaaattaccc tcgcttgagt ggttaaaaat agtggcgact
     5101 gatgagcaca ttcttgcctc attagaaaaa tatcacgagc catatgccat ttttgatgat
     5161 tactattgtg gtgcgatatg gtcagccacc gtattacagg agcaaggtgt tgcagccctg
     5221 ccccgatttg ctccctatgc cgcaagtgac tactgcgccg atgtgttgcg tcatatcaat
     5281 catccgttcg cattaacact gcttatacgt gtagccgggc atactaaacg ctgtcacgat
     5341 cggatgacga aagccattgc tgcgttccca catgcagcaa tggcggcact gacggaactt
     5401 cttgggcaaa aagaagagaa cagttggcgc attatgctaa tgacaatgct tatctcacaa
     5461 ccagcactgg cagaacaggt cattccctgg ctctcgacac ccgcagttgc cgtactgaaa
     5521 tcatgccagc aacaactgac acagccctca aaccatgcca gcgccgatct actgccagcc
     5581 gtagtagtct cccctccctg gctttcgaaa aagaaaaaat cgccgattcc ggtgctggat
     5641 ttagcgccat taggcattga gccaatctgt tatctgacag aagaaatcag taatcaactt
     5701 ttggcgaaat atatctggta ttcaaaacac atcacggtta gccatgaaga aagtactacc
     5761 aacctgttgg caaggatggg ttttcaacga cggatcgctg gtacatatat taaagctccc
     5821 gaagcggtag ttgaggcatg gctaaatgaa gattattcaa ccttactaag tgaatttaag
     5881 gtgtttcatt cacctaccgg gcattattgg cagttgggga ttttgacaac attgccgctg
     5941 gagaaagcag taaaagcatg gaatgccctt accctatctc cacataccga taccgaatac
     6001 gccatgttac attttggact caaagggtta cctgggttag taaactcact tgcacgctat
     6061 ccacaagaag ccttgcccat cacgaattac ttcgcagcga gtgagctggc tcctgccgtc
     6121 gcccgtgcct tcaacaaact gaaaacgcta cgcgaaaacg cccgtagctg gctgttgaaa
     6181 tacccggaac atgcccttac cggcctgctg cctgcggcgc tcggcaaagc cggtgaagca
     6241 caggataacg cccgcgctgc cttgcgtatg cttaccgaaa acggtcatca gccattactg
     6301 caagaaatcg cccgacgtta taaccagccg gaagtaaccg atgcggtgaa cgctctgctt
     6361 gcgctcgatc ccttagataa tcacccgaca aaaatcccca ctcttccggc cttttatcag
     6421 ccatcgctct ggacgcgccc ggtattaaaa gcaaatgccc aatcactgcc agatagcgcc
     6481 ctcctccacc tcggtgaaat gctccgcttc cctcaggaag aggctctgta tccgggatta
     6541 ttgcaggtga aagacgtctg ttccgccgac tcactggcgg gatttgcctg ggatctgttt
     6601 accgcctggc agaccgctgg cgcgccgtcg aaagagagtt gggcgttcac tgcgttaggc
     6661 gttctcggta acgatgacac cgcccgcaaa ctgacgccat taatacgcgc ctggcctggt
     6721 gaatcccagc ataaacgcgc caccgttggg ttggatattc tcgctgctat cggtagtgat
     6781 atcgccctta tgcagcttaa cggcatcgcc cagaaactga aattcaaagc attacaggag
     6841 cgggcaaaag aaaaaattgc cgacattgcc gagagccgcg aactcacggt ggcggagctt
     6901 gaagatcggt tagcaccgga tctcggtctg gatgataacg gttcgctgct gctggatttt
     6961 ggcccacggc agttcaccgt cagctttgat gaaaccttaa aaccgtttgt gcgtgatgtt
     7021 tccggcagcc gcctgaaaga cctgcccaaa ccgaacaaaa gcgatgatga atcgcggtcg
     7081 aacgatgcgg ttaaccgcta caaattgctg aaaaaagatg cgcgtaccgt cgccgcccag
     7141 caggtagcaa ggctggaatc cgccatgtgc ctgcgccgcc gctggtcgcc ggaaaacttc
     7201 cagctcttcc tggttgagca tccgctggtt cgccacttaa cccgccgtct gatttggggc
     7261 gtttatagcg ccgaaaacca gctactggct tgctttcgcg tagcagaaga taacagctac
     7321 agcaccgctg acgatgatct tttcaccctg ccggaaggcg atatctctat cggcattcct
     7381 cacgttctgg agatatcacc gacggatgcc gccgcctttg ggcagctttt tgccgactat
     7441 gaactgttac cgccgttccg ccagctcgac cgtaacagtt acgccctgac agaaaccgag
     7501 cgcaatgcca gcgaattgac ccgctgggca ggcagaaaat gcccgagcgg tcgggtcatg
     7561 ggactggcga ataaaggttg ggtgcgtggt gaaccgcagg atggtggctg gatcggctgg
     7621 atgatcaaac ctctgggccg ctggtcgtta atcatggaaa tcgatgaagg ctttgcagtt
     7681 ggtatgtcgc cagccgaact tagcgccgag caactcttaa gcaagctgtg gctatgggaa
     7741 ggcaaagcag aaagctatgg ctgggggagt aattcaacac aggaagcgca gttctccgta
     7801 ctcgatgcca tcaccgccag cgagctaatt aacgatattg aagccctgtt tgaataagga
     7861 aagagcatgg acaaggaatt accgtggctg gcggataacg cccaactgga actgaaatat
     7921 aaaaaaggca aaacgccgct cagtcatcgt cgctggccgg gcgaaccagt gtccgttatc
     7981 actggaagtc tcatccagac attgggagat gaattactac aacaagcagg ccagaaggaa
     8041 aatatcacct ggaattatga taaatgctca ctggagtggc agtccgccat ccagcaggca
     8101 atcaatttga ctggcgagca taaaccgtca atcccagccc ttaccatggc ggctctgatc
     8161 tgtatcgcgc aaaatgacag ccagcagtta cttgatgaaa tcgtgcaaca agaggggctg
     8221 gaatatgcga cggatgtagt gatagcgcgt cagtgcattg cgcgacgtta tgagagtgac
     8281 tcactggtcg tcactttgca gtatcaggac gaggactatg gctacggtta tggctccgcg
     8341 acctataacg atttcgatct ccgtttgcgt aagcatctgt cgctggcaga ggaaagttgc
     8401 tggcagcgtt gtgccgataa actcattgcc gcactgccag gaatacccaa aattcgccgt
     8461 ccttttattg cgctgatcct cccggaaaaa cctgaaatag ccaatgaact cgccagtctt
     8521 gaaagctctc ggtcaagtct ccattcaaag gagtggttaa aggtcgttgc tactgataat
     8581 acggcggtaa aaaaactcga acgatactgg ggcctggacg tatttagcga tcgcgaagcc
     8641 agctacatgt cacaagaaaa ccgcttcggc tacgcggcct gcgcctccct tttgcgcgaa
     8701 caaggactgg cagccgttcc gcgcctggca atgtatgccc ataaagaaga ttgcggcagt
     8761 ctgctggtac aaattaacca tccgcaagtc atccgcacct tgctactggt ggctgataaa
     8821 aacaaaccca gcctgcaacg tgtggctaaa tacagtaaaa acttccccca cgcgacgctc
     8881 gccgcactgg cagaactgct ggcgttaaaa gaaccaccag cccgcccagg ttatccaatc
     8941 atcgaagaca aaaagctgcc tgcacagcaa aaagcacgcg atgaatactg gcgtacgctg
     9001 ttacaaacgc tgatggcatc gcagccacaa ctggcagcag aagtgatgcc gtggttaagt
     9061 actcaagcca gggcagtgct gaatagttat ttatcggcac cgccaaaacc ggttattgat
     9121 agcaccgata acagcagtct gcctgaaatg ctcgtttcac caccgtggcg tagtaagaaa
     9181 aaaatgacag ctccacgtct tgatttggca ccgctcgaat taactccgca aatttactgg
     9241 caaccaggcg aacaagagag gcttgccgcc actgagtctg cccgttattt cagcacggaa
     9301 tctcttgcgg aacgcatgga acaaaaaagt ggacgagttg tattacagga actgggtttt
     9361 ggggatgatg tatggctgtt tctgaattat atactccccg gaaaactgga tgctgcacgc
     9421 aattcactca ttgttcagtg gcattactac caggggcggg ttgaagagat cctgaatggc
     9481 tggaactccc cgcaagcaca attagcagaa caggcgctcc gcagcggtca catagaagcg
     9541 ttaattaaca tatgggaaaa tgacaacttc tcacgttatc gtccggaaaa gagtgtctgg
     9601 aacctgtatt tattggcaca gttgccgcgt gagatggctt tgaccttctg gctgcgtatc
     9661 attgagaaaa agcatctgtt cgcgggtgag gactattttc tcagtatcct cggattggat
     9721 gcgctaccag gtctgctgtt ggctttttca catcgtccaa aagaaacatt tccgttaatt
     9781 ttaaattttg gcgcaacaga actggcgctg cctgttgccc gcgtctggcg acgtttcgcg
     9841 gcgcagcgtg atctggctcg ccagtggatt ttacaatggc cggaacatac ggctagtgca
     9901 cttatccctc ttgtctttac caaacccagc gataatagcg aagccgcatt acttgccctg
     9961 cgtttactgt acgaacaggg acatggcgaa ttgctacaaa ccgtggcaaa ccgctggcag
    10021 cgtacagatg tatggtctgc cctggagcag ttgcttaaac agggtccaat ggacatttac
    10081 ccggcacgca ttccaaaagc ccctgatttc tggcatccgg caatgtggtc caggcctcga
    10141 cttatcacta ataatcagcc tgttaccggt gacgctctgg aaattatcgg cgaaatgctg
    10201 cgctttaccc aggggggacg tttttatagc gggctggaac aactgaaaac gttctgccag
    10261 ccacaaacgc tggcagcttt tgcctgggat ctcttcactg cgtggcaaca agctggtgcc
    10321 cccgcaaaag acaactgggc atttctggcg ttaagtctct ttggtgacga aagcacggca
    10381 cgggatctga cgacacagat cctcgcctgg ccacaagaag gcaaatctgc ccgtgctgtc
    10441 agcggcctga acatccttac cctgatgaat aatgatatgg cgctgataca gctgcatcat
    10501 atatcgcaac gggctaaatc ccgcccctta cgtgataacg cggcggaatt tcttcaggta
    10561 gtcgcagaaa atcgcgggct aagccaggaa gagctagcgg acagattagt cccaaccctg
    10621 ggccttgatg atccgcaggc gttgagtttt gattttggtc cccggcagtt taccgttcgc
    10681 ttcgatgaaa acctcaaccc ggttatcttt aatcagcaaa acgttcgcca gaaaagcgtt
    10741 ccccggttgc gcgccgatga cgatcaactg aaagcgcccg aggcactggc ccgactaaaa
    10801 gggctaaaaa aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct tgaagctgcc
    10861 ctacgtacca cccgacgctg gtcgctggca gattttcatt ctctgtttgt taatcatccc
    10921 tttacccgtc tggttaccca gcgattaata tggggggttt atccggcaaa tgaaccgcgt
    10981 tgtttactca aagcctttcg tgtggccgca gagggggagt tctgcaatgc gcaagatgag
    11041 ccaattgacc tgcctgcgga cgctctgatt ggcattgccc acccgttaga aatgacagta
    11101 gaaatgcgca gtgaatttgc acagcttttt gccgattacg aaattatgcc gccttttcgc
    11161 cagttgtcgc gccgcacggt gctgctcaca cctgacgagt caaccagtaa cagcctgact
    11221 cgctgggaag gtaaatccgc taccgttggg cagcttatgg gaatgcgata caaaggctgg
    11281 gagtcaggtt atgaggacgc atttgtctat gacctgggtg agtaccggct ggtccttaag
    11341 ttttcacccg gttttaacca ctacaatgtt gatagcaaag cgctaatgag cttccgttct
    11401 cttcgagtgc accgtgacaa taaatccgtc acttttgccg aacttgatgt gtttgatttg
    11461 agtgaggcgt taagcgcacc tgacgtcatt ttccattaaa aacacagcgg gcagtgatgc
    11521 aactgcccgt tatcaacacg acttaccagc ggggaaagcg atgatcgtgc aaaaagagct
    11581 ggttgctatt tacgattatg agatccctgt acctgaaaat ccgttttcct tcagacttga
    11641 gatccataaa tgctctgaat tatttacagg ttccgtctat cgactggagc gattccggct
    11701 acgtccaaca tttcatcaac gtgatcgaga agatactgac ccgctaataa atgatgcgtt
    11761 gatttatata agagatgagt gtattgatga gcggaaatta cgaggtgaat cacctgaaac
    11821 tgtaatagca atttttaatc gtgaactaca gaatatattc aaccaagaaa tagaataata
    11881 tactctagat aattaaagtc gcagcacatg caacttgaag tatgacgagt atagctttct
    11941 taaaggggga aacggtattc ccccttagat gcgtttcaat ttgcccgtta cttcaacaaa
    12001 tcaatcaacc gtaacagagc cactaataac tccagtacgg caatgagcac ggttaacttt
    12061 tgtgtcagcg tgtccatccg ctctccttat taaggagcgc aacaccagca ccttaccctt
    12121 acactgcctg ttgatactgg tttacgtatg ttggtacgaa acgttgctct ccggatcagg
    12181 gcaccgcctg cggcaccacc cgtccggcag ccaggactta aaattggtcc gatccggcac
    12241 aacctcccaa cgggcaggat tataaatctc actcactgaa ttgcaatgga gtgtggatat
    12301 ggttataatt tattcgttgg tacgaaatgt tgcacaatca gcaagttagc gtcactgtag
    12361 taaataaaag aacctgcctc accagcaggt ttttttatt
//
LOCUS       DAZFWL020000134        12217 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFWL020000134 DAZFWL020000000
VERSION     DAZFWL020000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.134"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(42..950)
                     /gene="galU"
                     /locus_tag="ACHKNP_RS19890"
                     /old_locus_tag="pgaptmp_003978"
     CDS             complement(42..950)
                     /gene="galU"
                     /locus_tag="ACHKNP_RS19890"
                     /old_locus_tag="pgaptmp_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGU4706057.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(1152..2165)
                     /gene="rssB"
                     /locus_tag="ACHKNP_RS19895"
                     /old_locus_tag="pgaptmp_003979"
     CDS             complement(1152..2165)
                     /gene="rssB"
                     /locus_tag="ACHKNP_RS19895"
                     /old_locus_tag="pgaptmp_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HGU4706058.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2257..3162)
                     /gene="rssA"
                     /locus_tag="ACHKNP_RS19900"
                     /old_locus_tag="pgaptmp_003980"
     CDS             complement(2257..3162)
                     /gene="rssA"
                     /locus_tag="ACHKNP_RS19900"
                     /old_locus_tag="pgaptmp_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HGU4706059.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            3275..3733
                     /locus_tag="ACHKNP_RS19905"
                     /old_locus_tag="pgaptmp_003981"
     CDS             3275..3733
                     /locus_tag="ACHKNP_RS19905"
                     /old_locus_tag="pgaptmp_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HGU4706060.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3783..4625
                     /gene="purU"
                     /locus_tag="ACHKNP_RS19910"
                     /old_locus_tag="pgaptmp_003982"
     CDS             3783..4625
                     /gene="purU"
                     /locus_tag="ACHKNP_RS19910"
                     /old_locus_tag="pgaptmp_003982"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HGU4706061.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     SDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4785..4869
                     /locus_tag="ACHKNP_RS19915"
                     /old_locus_tag="pgaptmp_003983"
     tRNA            4785..4869
                     /locus_tag="ACHKNP_RS19915"
                     /old_locus_tag="pgaptmp_003983"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4819..4821,aa:Tyr,seq:gta)
     gene            4917..5048
                     /locus_tag="ACHKNP_RS19920"
                     /old_locus_tag="pgaptmp_003984"
     ncRNA           4917..5048
                     /ncRNA_class="other"
                     /locus_tag="ACHKNP_RS19920"
                     /old_locus_tag="pgaptmp_003984"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5232..5909)
                     /gene="narI"
                     /locus_tag="ACHKNP_RS19925"
                     /old_locus_tag="pgaptmp_003985"
     CDS             complement(5232..5909)
                     /gene="narI"
                     /locus_tag="ACHKNP_RS19925"
                     /old_locus_tag="pgaptmp_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU4706062.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(5909..6619)
                     /gene="narJ"
                     /locus_tag="ACHKNP_RS19930"
                     /old_locus_tag="pgaptmp_003986"
     CDS             complement(5909..6619)
                     /gene="narJ"
                     /locus_tag="ACHKNP_RS19930"
                     /old_locus_tag="pgaptmp_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU4706063.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(6616..8154)
                     /gene="narH"
                     /locus_tag="ACHKNP_RS19935"
                     /old_locus_tag="pgaptmp_003987"
     CDS             complement(6616..8154)
                     /gene="narH"
                     /locus_tag="ACHKNP_RS19935"
                     /old_locus_tag="pgaptmp_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU4706064.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(8151..11894)
                     /locus_tag="ACHKNP_RS19940"
                     /old_locus_tag="pgaptmp_003988"
     CDS             complement(8151..11894)
                     /locus_tag="ACHKNP_RS19940"
                     /old_locus_tag="pgaptmp_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HGU4706065.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         2920 a         3300 c         2995 g         3002 t
ORIGIN      
        1 gattgctcaa cgccgtttcg tggataacac cgatacggat gttacttctt aatgcccatc
       61 tcttcttcaa gccaggcttt aaattccgtg ccaagggtgt tatgacgaat accgtattca
      121 acgaaggcct gcatgtaacc taatttatta ccgcagtcat ggctcttccc tttcatatga
      181 taggcttcca ccgtttcttt ttcaatcagc atatcaattg cgtcggtgag ctgaatttca
      241 tcaccagctc ccggaggggt ttttgccagc aacggccaaa tatccgcgct aagtacgtaa
      301 cgacccacaa tagcgagatt agacggcgca acatccgctt tcggtttttc taccacacca
      361 accatcggta cgctttcacc cggcgctaat tcaacgcctt tgcaatccac aacgccatat
      421 gcggtcacat cagcaaccgg ttcaaccatg atctggctat gacccgtttc atcaaagcgg
      481 cggatcatct ctgccaggtt atcctgtgac aaatcggatt catattcatc cagaataaca
      541 tcaggcaaaa taacagctac cggttcatca cccactaccg ggtgagcaca caataccgcg
      601 tgtcccaggc ctttcgccag accctgacga acttgcataa tagtcacgtg cggtggacaa
      661 atagactgca cttcatcaag cagttgacgt tttacacgtt tttccagcat tgcttccagt
      721 tcaaaactgg tatcaaagtg gttttcaata gagtttttag atgagtgtgt aaccagcaca
      781 atttcagtaa tgccagccgc aatacattca ttcacgacgt attgaattaa tggcttatcg
      841 acaagtggca gcatctcttt cgggatggct ttcgtcgccg gcaacatcct ggttcctaat
      901 cccgcaacgg ggataacggc ttttttgact ttcgtattaa tggcagccat ttaaattctc
      961 ctggactgtt cgtgttttga acgtgttcat atttctgtat cgcatcccag tatatcagca
     1021 ccccgaaggg gctaagttct gaaaaggagg cgagttagca taattaagac aattacttat
     1081 acgaatggcg acgatagtac caccagccga cattagcagg taatgcaaat ttagcccgcg
     1141 ttatcgtttg ctcattctgc agacaacatc aagcgcagtc gaccaccggt tccccatatt
     1201 tggcattgcc aggcatcgca tcgctggctc aattgattca aataagcgtt acctaaagtg
     1261 cctaacggaa caccattact gatttgcacc tggtgttcgc cggtatttaa cgtcgcattc
     1321 agacccgcag aaaccagaat gagatttttc agttcgcgat gataatagcc aactaatagc
     1381 ggaaactgcc ccggcagatt ggcctgacga agtaaatggt ttacctgctt caataacgcg
     1441 cccaactctg gcaaccgttg attttggtgt gcaagctgtt cctgtaataa tccgttaaac
     1501 aatgcgcgta ataacaaggc agcaagtacg ccattatgtc cagctcgggt gacatcaagg
     1561 caataaaatg ccagatcgtt ttccgaaagt gcggcaatat caagcaccag gccgggtttg
     1621 tccgcggcaa ccaattgacg ataattaacc cggcaatggg aaatcacctg ctgaaccggc
     1681 ggttgtagtt cctgtaataa tttcgccgct gcggcagggt tatcaaccat tgcatcccag
     1741 tcgcgaaaaa gcctttcctc ttcctcaacg cgcgaattaa acatgctggg atagagacag
     1801 gcaaaaacca tctcgcgcaa gcgattcaaa tctttaactg gtttcagcaa aacatcttca
     1861 acgcccagac gtaacgcttt ggcaatatct gccatatttt cggtggcaga tatcaccaga
     1921 accggtgtct ggtcacctct gttgcgtata tgttccagca gtttaagccc gttcattcgt
     1981 ggcatcgcga tatcacatat catcaggtct ggagtgaaac ctcccagcaa ctcaagggca
     2041 tccaccccat cagccgccag taccgttgtc gctcccaatg aggaaaacca cgaatccaga
     2101 agcgagcgaa ataccggctc atcttcaaca atgagaatct gttttccgac caatggctgc
     2161 gtcatgttct ctcccctgac tggctttact caatagtggc atgctattgc cacctgcgcc
     2221 tgtcagaatt aacttaagtg tagataaaaa ttctggtcaa atgttggtgc gtaccaacgg
     2281 caaaagttcg tccattttct tttccactgc cagctgtccg gccgcaatgg cagcgtgcgc
     2341 gcgatggaaa tcaagcgtag atatttgcgg gcaaacaggt tgaatcagaa tatcgggcgg
     2401 atcacctgcc atgcggttcc ttttaaggcg gttctccagc acctggatag aagtggtcat
     2461 aatctctgtt gccgttggcg ctgtcaccgc ccgacgtgtc gttatgctgc ctaacctttc
     2521 tttcagacgc gcatgccacg gcagagaatc accattctcg ctattttctt cactgacatt
     2581 aaaggagagc aaatcttgtt gcatcaaatg agcatcgtgc tgcaggtcaa ccgctatcac
     2641 aatatcagcc cccaatgcac gcgtgaggga aataggaatt gggttaacga ctgctccatc
     2701 aaccagccag tagccgttat gtgcaacagg tgccatgagt cctggaatac tgcatgatgc
     2761 gcgaatagca agatggagat cgccttcagt aaaccataat tcacgtcccg tacttaaatt
     2821 ggtggcaaca gccgcaaagc gacgggaaca attttcgatc tctgtttccg gcattatttc
     2881 gcggtattga ttgaagacac gctcgccgcg cagtaaccca ccgcgctgcc aggagagatc
     2941 catcaggcgt aaaacatccc aataactgaa agaggtcacc caatcttcca gcgcagataa
     3001 tcgatcgcat gcataggcag cgcccaccag cgaaccaatt gaacatcctg caacgatatc
     3061 aatttcaata cccacttttt ttagcgcatt aataacgcca atatgcgacc aacctctcgc
     3121 cgcgccagat cccagcgcca gccctatttt tatctttctc attattcccg caagattccc
     3181 ctggaatgca atcgtagcca catcgctact gctcggttaa catagtgcta ctcaggcggt
     3241 taatacgccg ttattgtttc cagggagatt atttgtgtct cagctttgtc cctgtggtag
     3301 tgctgtcgag tatagcctat gttgccaccc ttatgtgtct ggtgaaaagg ttgcacctga
     3361 tccagaacat ctcatgcgtt cgcgttactg cgcttttgtg atgcaagacg cagattattt
     3421 aataaagacc tggcatccct cttgtggagc agcggcgtta cgtgccgaat tgatggccgg
     3481 atttgcgcat actgaatggt tgggattgac cgtatttgaa cattgttggc aggacgccga
     3541 caatatcgga tttgtcagtt ttgttgctcg atttaccgaa ggtggtaaaa caggtgcaat
     3601 aattgaacgt tcgcgatttt tgaaagaaaa cggtcaatgg tactatattg acggtacacg
     3661 tccgcagttt ggtcgcaacg atccctgccc ttgtggttca ggtaaaaaat ttaaaaagtg
     3721 ctgcggccaa taatggttga cggtacggtt tagcaaacac tctcaacaag gtttttccag
     3781 caatgcattc actccaacgt aaagttctgc gtactatttg tccggaccaa aaaggtctga
     3841 tcgcacgtat taccaatatt tgctacaagc acgagttaaa tatcgtacag aacaatgaat
     3901 ttgttgatca ccgtaccggg cgctttttta tgcgcacgga actggaaggg atttttaatg
     3961 attccaccct gctggcggat ctcgatagcg cattgccaga aggctccgtg cgtgagctga
     4021 atcctgccgg tcgtcgccgg atagtgattc tggtcactaa agaagcgcat tgccttggcg
     4081 atttgttgat gaaagccaat tacggcggcc tggatgtcga aatcgcggca gttattggta
     4141 accacgatac tttacgttct ctggttgagc gttttgatat tccgtttgag ctggtaagcc
     4201 atgaagggtt aagccgcaac gagcacgatc aaaagatggc ggacgccatt gatgcttatc
     4261 aatctgacta tgtggtgctg gcgaagtata tgcgggtatt aacgccggaa tttgtggcac
     4321 gcttcccgaa taaaatcatc aatattcacc attcattcct gcctgcgttt atcggcgcac
     4381 gtccttatca ccaggcctat gaacgtggtg tgaagattat tggcgcaacc gctcactatg
     4441 tgaatgacaa tctggacgaa ggcccaatca tcatgcagga cgttattcat gtcgatcata
     4501 cctacacagc tgaagatatg atgcgcgcag gtcgtgacgt cgagaaaaac gtcttaagtc
     4561 gtgcgctata caaagtactg gcacagcgcg tctttgttta cggtaatcgg acgattattc
     4621 tttaatcgcc agcaaaaata actggttacc tttaatccgt tacggatgaa aattacgcaa
     4681 ccagttcatt tttctcaacg taacacttta cagcggcgcg tcatttgata tgatgcgccc
     4741 cgcttcccga taagggagca ggccagtaaa aagcattacc ccgtggtggg gttcccgagc
     4801 ggccaaaggg agcagactgt aaatctgccg tcatcgactt cgaaggttcg aatccttccc
     4861 ccaccaccat cacttttcaa aagtccgtaa actctcaaac gaatccgcaa ttaaatattc
     4921 tgcccaagcg gggaaggatg agaagcttcg accaaggttc gacttgagcg ccagcgagag
     4981 agcgttgccg caggcaacga cccgaagggc gaagcgcgca gcgttgagta atccttcccc
     5041 caccaccatc acttccttaa ataatcaaaa cctgttactc cgagctaatt ttgcccgatg
     5101 gcaccacgtt taccgggcct acaatatcct cccctgtagg tcggataagc gaagcgcatc
     5161 cgacaactca ccccgcaccg tgcccataaa aaaaccccgc cgaagcaggg tctatgtgaa
     5221 ctaaaattcg cttagtgacg agcgcgcacc agctggtact tacgtgtcag atactccacc
     5281 ggtacgctcc agatgtgtac cagacgcgag aacgggaaca gcaggaataa cgtcatcccc
     5341 agcaccaggt gcagacggaa gataaacgcc acaccatcga ggtgttgaga agcgccaccg
     5401 tggaaggtca ccaccgactg cgcccagcca accagtttca tcatctcgct accgtccata
     5461 tgctgagcgg agaacggaat ggtcaacagg cccagcgcgc actggataac gagcagcgac
     5521 aggatcagga tatccgctcc ggtagtggtt gcacgcacgc gtgggctgaa cagacgacgc
     5581 ttcagcaaca gcacgccgcc aatcagacac agcacgccgc tggcaccacc agcaaacatc
     5641 gccattttct gtttcacttc aatcggcagc caggcttcat acatccagtg cggcgtcagc
     5701 ataccgaaga agtgaccaac aaaaatcccc agaataccga tatggaacag gttcgacgcc
     5761 aggttcatcc ctttgcgatc cagcatctgg ctggacgccg cgcgccaggt gtactgcccg
     5821 tagtcataac gcagccagct accaatcagg aagaccgccc cggctatgta cgggtagata
     5881 tcaaagaaga acatattcag gaattgcatt agtgctgtcc tccggtggtg atattcagat
     5941 attgcggcgc gacggcaccg gcaaagcgac gctgatgagc agtgattgct gaatcaccgc
     6001 agcctttgtc agcaaagaat ttaacctgct cttcttccca gacagcatcc agcgcctgcg
     6061 gcgtatcatc gcgcgcttcg tcggcaattt tttccgccac tttgtcgctg tcgatagcgg
     6121 tattggccaa tttcagcagc agatcaaaca tcacggcata acggctttca cgctgttgca
     6181 gacgcgcgct cagcaatgcc agaatcggcg cgatatcttt caaaccttcc acggcttcgc
     6241 tttgcggcag ctgcgacagg tactccagat acagcggcag atggtccggt aattcgcggc
     6301 tgtttaactg caagccgtgc tgctcgtact gcgccagcag gtccaccatc gcctggccgc
     6361 ggtcacggga ttcaccgtga acatgttcaa atagcaacag tgacgtggcg cggccacggt
     6421 cgaacagttc gctgtactgg gcctgggcat cgagcggatc catcgccgtt aaatcgcgca
     6481 ggaaaatgcc cagcgcatgg gcatcttctt tcgacagatt tttcgacgca gcaatcgcct
     6541 caaacatctc ttgttgatgc tgccataagg cagcatccgg atattcaagg agacgcgata
     6601 caatcacgag ttcgatcatg gatgcggctc cgttttgctg gtcacatcga tggcatcgat
     6661 acgacggctg ttgaacagat tgaatttggt atctgaaccg tggcagccat caccaaaggt
     6721 aaagccgcag ccatttttct ccgggaaggc ttcccgtgcc agttcacgat gactactcgg
     6781 caccacaaag cgatcttcgt agttagcaat cgccagataa cggtacatct cctgtgcctg
     6841 ggcttcggtc agaccgacct cttccagcgc acgggtatca actttaccgt caacggtttc
     6901 agcacgtttg taatgacgca tcgccaacat acgtttcagt gcgcgcagta ccggtttggt
     6961 atcaccggcg gtcagcagat tcgccagata ctgtaccgga atacgcaagc tttcgacgtc
     7021 tggcagaatg ccgttgctac ccagctcacc cgcgtctgct gcagactgaa tcggagacag
     7081 aggcggcacg taccagacca tcggcagtgt gcgatattct ggatgcagcg gcagcgccag
     7141 tttccattcc attgccattt tataaaccgg cgactgctgt gcggcttcaa taacgctcag
     7201 cggaataccg tctttaatcg cctgctcgat gactttcgga tcgttcggat cgaggaacac
     7261 ctccagctga cgctggtaaa gatctttctc gttctcggtg ctggctgcac gttcaatcgc
     7321 gtcggcatcg tacagcagca cgccaagata acggatacga ccgacacagg tttctgagca
     7381 cacggtcggc tgacccgctt caatacgcgg atagcagaag atgcacttct cagacttacc
     7441 gctcttccag ttgaagtaga tttttttgta cgggcatccg gtgatgcaca tacgccagcc
     7501 acggcattta tcctggtcga tcaggacgat gccatcttct tcacgcttgt aaatcgcacc
     7561 gctcgggcag gtcgccacac atgccgggtt caggcagtgt tcgcacaggc gcggcaaata
     7621 catcataaag gtgttttcga actggctata catcgccttc tggatgttgt cgaagttctt
     7681 gtctttcgcc agtttgtcaa actcaccacc caggtcatct tcccagttcg gccctttttc
     7741 gattttcgcc atccgttccc cggtaatcag cgaacgcgga cgggcaatcg gctgcgattt
     7801 gctgccttcc ggcgcggtat gcaggttctg atagtcaaaa tcgaacggct cgtaataatc
     7861 gtcgatcccc ggcagatgcg ggttagcgaa gattttaccc agcaacatgg cacggttacc
     7921 catgcgcggc tgcagtttgc cgttgatttt acggatccag ccgcctttgt atttttcctg
     7981 gttttcccag tcagtcggga agccctgacc cggcttggtt tccacgttgt tgaaccacgc
     8041 gtattccacg ccttcacggc tggtccagac gtttttacag gtaactgaac aggtgtggca
     8101 gccgatgcac ttatcgagat tcagcaccat gccgacttgt gaacgaattt tcattttacg
     8161 ctctcctgta cctggtcatt gccttcgcca tctaaccagt caatgttctt catcttacgc
     8221 actacaacaa actcatcgcg gttagaacct acggtgccgt agtagttaaa gccgtatgcc
     8281 agatgggcat agccgccgat catatgcgtc ggtttcggcg tgatacgggt gaccgagtta
     8341 tggataccac cacgctgttg ggtaatttcc gaaccaggca ggttaacgat acgttcctgc
     8401 gcgtggtaca tcatggtcat ccctgccgga acacgctggc tgacaaccgc acgggcagtc
     8461 agagcaccgt tgctgttgaa gacttcaatc cagtcgttat cggcgatacc cagatctttg
     8521 gcatcggctt cactcaacca gaccaccgga ccaccgcgac ctaaagtcag catcagcagg
     8581 ttgtcgctgt aggtggagtg gataccccac ttctggtgcg gcgtcaggaa gttgagcgct
     8641 ttttccgggt tgccattgga tttctggccc atcacttctt tcaccgaacg ggtgtcgatc
     8701 ggcggacgat aaaccagcag gctttcaccg aaatcacgca tccattggtg atcctgatac
     8761 agctgctgac ggccagagag cgtacgccac gggatcagct cgtgaacgtt ggtgtaaccg
     8821 gcgttgtaag aaacgtgttc atcttcaagg ccagaccagg tcgggctgga gatgattttg
     8881 cgcggctgtg cctgaatatc gcggaagcga atcttctcgt cttctttatt cagcgccaga
     8941 tgcgtatggt cacgaccggt aaactcgctc agggcagccc aggctttcac ggctacctga
     9001 ccgttggttt ccggtgccag cgtcaggatc atctctgccg catcaattgc ggtgttcagc
     9061 atcggctggc ctttcgccgg accttccgct ttggtgtagt tgagcttacg cagcagatcc
     9121 atctcgctct gggtgttcca ggcaatccct ttaccgccat taccgatttt ctccatcagc
     9181 gggccgatag aggtaaagcg ttcgtaagtc gccggataat cgcgctctac gaccataatg
     9241 tgtggcgcgg ttttaccagg gatcaggtcg cactcgcctt ttttccagtc tttcacatcc
     9301 agcggctgcg ccagttcagc ggcagagtcg tgctggatag gcagcgtgac gatgtcggtt
     9361 tctttaccca gatgaccgac gcacacttcg gagaatttct tcgcgatggc tttgtaaatt
     9421 tcccagtcgc ttttcgcttc ccaggccgga tcgaccgccg cagacagcgg gtgaataaac
     9481 ggatgcatat ccgaagtatt catgtcgtct ttttcgtacc aggtcgccgt cggcaaaatg
     9541 atgtcggaat agagacaggt gctcgacaga cggaagtcca gcgtaaccac cagatccagc
     9601 ttgccttcca gaccattgtc ctgccagtcc acttcttccg gcttcacgcc gccctgttgc
     9661 cccagatctt taccctggat accgtgctcc gtccccagca ggtacttgag cataaactca
     9721 tgacctttac cggaagaacc gagcaggtta gaacgccaga tgaacaggtt gcgcgggtgg
     9781 tttttaccgt tttctggttg ttctgccgca aaacggatgg aaccctcttt cagggatttc
     9841 accgtatagt ccaccggatt catcccggct ttttccgctt cgccagcgat agtcagcggg
     9901 ttagtgccta actgcggtgc agacggcagc cagcccatgc gttccgcacg gacgttaaag
     9961 tcgatcaagt gtccggtata gcgggatttg tccgccatcg gtgacagcaa ctcttccgcc
    10021 gtgacggttt cataacgcca ctggctggag tggttataga aataagaagt gctgttcatg
    10081 tgacgcgccg gacgctgcca gtcaagggca aacgccagcg gctgccagcc ggtttgcgga
    10141 cgcagttttt cctgacctac atagtgcgcc cagccgcccc cgctctgacc gacacagccg
    10201 cagaaaatca gcatgttgat cagaccacga tagttcatat cgaggtgata ccagtggttc
    10261 agccccgcac cgacgataat catcgaacga ccgtgcgttt tatcagcgtt atcggcaaat
    10321 tcacgggcga tgcgaataat ctggctgcga gaaacgccgg taatctgctc ggcccaggcc
    10381 ggggtataag ctttcacatc gtcatagctg gttgcacagt taacgtcgtt caggccacgt
    10441 tccagaccgt agtttgccag cgtcagatca taaacggtgg tcaccagggc ggtgctgcca
    10501 tcagccagtt gcaggcgttt caccggcagt ttgtgcagca gcacgttttc cagttccact
    10561 ttgttgaagt gttccgtgcc gtcgccacca aagtacggga agcctacctc agcgatctca
    10621 tcctggctac ccagcaggct cagttgcagc tcggtttctt cgccagtttt gccgtcgcgc
    10681 tgctcaagat tccatttgcc cttctcgccc cagcggaagc caatagaacc gttcggcgca
    10741 accatttcgc cattggtatt aaaggcgaca gttttccatt ccggattgtt ttcctggccc
    10801 agcgcatcaa ccagatcagc agcgcgcagc atacgacctg cagcgtagta accgtcgcgt
    10861 tcttccagca tcaccagcat cggcatgtcg gtgtagcgac gcacatagtc ggtgaaatac
    10921 tggcttgggt tgtcgaggtg gaattcacgc agcattacgt ggcccatcgc cagcgccatt
    10981 gccgcatcgg tgccctgttt cggtgccagc cacagatcgc acagtttggc gatttcagcg
    11041 tagtctggtg tgacggcaac agttttggtc cctttgtaac gcacttcagt aaagaagtga
    11101 gcatccgggg tacgcgtctg cggtacgttt gacccccagg cgatgatgta gctggagttg
    11161 taccagtcag cagattccgg tacgtcagtt tgctcgcccc aggtttgcgg agacgcagga
    11221 ggcaagtcgc agtaccagtc gtagaagctt aagcaagtac cgccaatcag cgagagatag
    11281 cgtgcacccg atgcgtaaga aaccatcgac attgccggaa ttggcgagaa accagcaaca
    11341 cggtccgggc cgtagttttt gatggtgtaa acgttagatg cggcgatcag ttcgttcacc
    11401 tcctgccagg aagaacgaac aaatccaccg cgtccacgcg cctgcttaaa gcttttcgct
    11461 ttatcggcgt cttcaatgat agaagcccat gcctcaaccg gatcgctatg cagcgccttc
    11521 gcttcacgcc acattttcat caggcgtttg cgcatcatcg ggtatttcag gcggttggca
    11581 ctgtaaagat accaggagta gctggcaccg cgcgggcagc cgcgaggttc atggtttggc
    11641 agatccggac gggtacgcgg atagtcagtc tgctgggttt cccaggtgac cagaccgttt
    11701 ttgacgtaga ttttccagct gcaggagccg gtgcagttta ccccgtgggt agagcggacg
    11761 attttgtcat gctgccaacg ctggcgatat ccatcctccc agtcacggtt ggtattgaga
    11821 agctggccat gcccatcggc aaaggtttca cccttctgtt tgaagtagcg aaaccggtcc
    11881 aggaatttac tcatcggttt tctcctgtgg gagcctgtcg gccctctgat aaatcgacat
    11941 tgctaatgtt taagggcaag gtaacgctct gaaacagcgt gggaattgat aacgatcaag
    12001 tgtgatgggg atgaaaaata aagtaaattc ctaagatacc cctttaggat aattaacagt
    12061 taaagcataa catgctgtaa attaagacgt tttatacctg tagttattta aagcatgaag
    12121 atttttatgc agatgggtat taaggagtat tccccatggg taacatatta atggctctta
    12181 atgaaatccg gcgaataagc catataacat tagtatg
//
LOCUS       DAZFWL020000135        12173 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFWL020000135 DAZFWL020000000
VERSION     DAZFWL020000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.135"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..809
                     /gene="yjiC"
                     /locus_tag="ACHKNP_RS19945"
                     /old_locus_tag="pgaptmp_003989"
     CDS             <1..809
                     /gene="yjiC"
                     /locus_tag="ACHKNP_RS19945"
                     /old_locus_tag="pgaptmp_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU4706066.1"
                     /translation="LQSQIITDNHFLHHPKVDSELTRKYERARLDTENIYLLPLARGN
                     NHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKITSALDEIY
                     GGICKKAHAATELAEGLRLNLFMKSPFDPVENYTVHEITLGPGCNVPGYAGTTIGYIS
                     TLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGNDPIIGIEA
                     YPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(950..1723)
                     /gene="uxuR"
                     /locus_tag="ACHKNP_RS19950"
                     /old_locus_tag="pgaptmp_003990"
     CDS             complement(950..1723)
                     /gene="uxuR"
                     /locus_tag="ACHKNP_RS19950"
                     /old_locus_tag="pgaptmp_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HGU4706067.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDV"
     gene            complement(1938..3398)
                     /gene="uxuB"
                     /locus_tag="ACHKNP_RS19955"
                     /old_locus_tag="pgaptmp_003991"
     CDS             complement(1938..3398)
                     /gene="uxuB"
                     /locus_tag="ACHKNP_RS19955"
                     /old_locus_tag="pgaptmp_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HGU4706068.1"
                     /translation="MTTIVDSNLPVARPSWEHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKVNAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3479..4663)
                     /gene="uxuA"
                     /locus_tag="ACHKNP_RS19960"
                     /old_locus_tag="pgaptmp_003992"
     CDS             complement(3479..4663)
                     /gene="uxuA"
                     /locus_tag="ACHKNP_RS19960"
                     /old_locus_tag="pgaptmp_003992"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HGU4706069.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5115..5207
                     /locus_tag="ACHKNP_RS19965"
                     /old_locus_tag="pgaptmp_003993"
                     /pseudo
     CDS             5115..5207
                     /locus_tag="ACHKNP_RS19965"
                     /old_locus_tag="pgaptmp_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5450..6352)
                     /gene="fimH"
                     /locus_tag="ACHKNP_RS19970"
                     /old_locus_tag="pgaptmp_003994"
     CDS             complement(5450..6352)
                     /gene="fimH"
                     /locus_tag="ACHKNP_RS19970"
                     /old_locus_tag="pgaptmp_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU4706070.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGSVLSNFSGTVKYSGSS
                     DPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTVDAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6372..6875)
                     /gene="fimG"
                     /locus_tag="ACHKNP_RS19975"
                     /old_locus_tag="pgaptmp_003995"
     CDS             complement(6372..6875)
                     /gene="fimG"
                     /locus_tag="ACHKNP_RS19975"
                     /old_locus_tag="pgaptmp_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HGU4706071.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(6888..7418)
                     /gene="fimF"
                     /locus_tag="ACHKNP_RS19980"
                     /old_locus_tag="pgaptmp_003996"
     CDS             complement(6888..7418)
                     /gene="fimF"
                     /locus_tag="ACHKNP_RS19980"
                     /old_locus_tag="pgaptmp_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HGU4706072.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7428..10064)
                     /gene="fimD"
                     /locus_tag="ACHKNP_RS19985"
                     /old_locus_tag="pgaptmp_003997"
     CDS             complement(7428..10064)
                     /gene="fimD"
                     /locus_tag="ACHKNP_RS19985"
                     /old_locus_tag="pgaptmp_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HGU4706073.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYFSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10131..10856)
                     /gene="fimC"
                     /locus_tag="ACHKNP_RS19990"
                     /old_locus_tag="pgaptmp_003998"
     CDS             complement(10131..10856)
                     /gene="fimC"
                     /locus_tag="ACHKNP_RS19990"
                     /old_locus_tag="pgaptmp_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU4706074.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(10893..11432)
                     /gene="fimI"
                     /locus_tag="ACHKNP_RS19995"
                     /old_locus_tag="pgaptmp_003999"
     CDS             complement(10893..11432)
                     /gene="fimI"
                     /locus_tag="ACHKNP_RS19995"
                     /old_locus_tag="pgaptmp_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4706075.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11497..12045)
                     /gene="fimA"
                     /locus_tag="ACHKNP_RS20000"
                     /old_locus_tag="pgaptmp_004000"
     CDS             complement(11497..12045)
                     /gene="fimA"
                     /locus_tag="ACHKNP_RS20000"
                     /old_locus_tag="pgaptmp_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4706076.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3005 a         3187 c         2901 g         3080 t
ORIGIN      
        1 cccttcagag ccaaattatt acagataatc acttccttca tcatccaaaa gtcgacagtg
       61 agttaacacg taaatatgaa cgtgcacgcc tggatacgga aaatatctat cttctgcctc
      121 ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt agaaaacttg
      181 atattagtaa agagtcctgg ccatttaatt atgtgacaga agcatgcaga gaatccgatg
      241 gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca gctttagacg
      301 aaatatatgg tggcatatgt aaaaaggcgc atgctgctac agaattagca gaaggtttac
      361 ggctaaattt atttatgaag agccctttcg atcctgttga gaactataca gttcatgaaa
      421 taacattagg ccctggttgc aatgttccag gttacgccgg aaccacgata ggatatattt
      481 caacccttcc agtttctcag gcaaaaagat ggaccaatga gcagccaaga attgatatct
      541 atattgatca gataataact gtctcagggg tagcaaattc ttccggattc gcccttgccg
      601 cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata gaagcctatc
      661 ctggtaccgc ggagatccat gcaaaaatgg gatataaagt tatccccggt gatgaagatg
      721 cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt gaattaaaaa
      781 atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact aatattcagg
      841 ttatacagat atttaaacaa taccctgcaa tttacaaagc aatactcatt gcgacttctt
      901 aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt cagacgtcga
      961 ctttatccag cggccatgaa tcaaacagat agccgtcaaa ataaatatcg tcaacgttcg
     1021 agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc agcttcgccg
     1081 ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc aaccactctt
     1141 tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga ttgttttccc
     1201 gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt gcttcggcaa
     1261 tagcgagatg gaactgcatg tcaccgcttt cgctgctgcc cggcgcactg gaagccagtt
     1321 cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg gtagcctgca
     1381 aagcggcaaa ctcggcgatg ttgctctcca ataactgccg cgcctgtaac agctcaaaag
     1441 gaccggcatc gttgcagacg ttggcatccg gactgtctgt gttctggctg cctgagttat
     1501 caagaacata gataccggca ccccggcgta cttccaccag ccctttgatc tccagcatga
     1561 tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt tcacgctccg
     1621 gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg atcatcgccc
     1681 cgacttcctg gtaaggtctt tgcgcagagg tggcagattt catggtgttg tgtccgttag
     1741 gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc ctacaacggg
     1801 caattttacg gcccccgtta atctcaaact ataccattag cactggaagt tggcacgtac
     1861 aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac cagtaagtct
     1921 gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg ttcgcacagc
     1981 tgctgatatg ccgccgtcac tgcgccaaca aagtcggcat tctgcggcag atcatcggca
     2041 aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata ctgcgcgttg
     2101 accttctgga actccgccag catcgggtcg accacgtcaa tggcattacc ctgctcatcc
     2161 acgccctggg tgtaacgcat ccagccagcc acgcccagcg ccaggtgacg ccagctgccg
     2221 ccgttttgca ggtgcagacg caccgggtcc agcagacgct gcggtaactt ctggctgccg
     2281 tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg ctcgatcagc
     2341 agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg cgcttgttcc
     2401 tgcatcatca aggagaaggc cgctttgcga taatccgggt tagtcatggt gtcggcaatg
     2461 gtttcatagc cgccgaggta accgaggtat gccaggaaag agtggctgcc gttcagcata
     2521 cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgcgcc cactttatcc
     2581 cagtccgggc gaccgttaac gaagttatct tcaatcaccc actgacggaa cggttcgcag
     2641 gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgcaa ggtttctggc
     2701 gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt ttcttcaatc
     2761 catgccgcca gctgcgggtc acgcgcctga gccagtccca gtaccgcgac cttcgccaca
     2821 tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa acgctttcaa ccctttttca
     2881 cgacgcagcc gcagggcttc gacgatgtaa ccaatcgcgg acttcggcgc agtcgggttt
     2941 tccagatcgt gcttgatcag tgggttattg agatccagct gaccgcttgc cgcatcagcg
     3001 cagtagcctt tttccgtgac tgtcagagag acaatcgccg tctgcggacg cgccatcgca
     3061 ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat cgaaccgata
     3121 attttcagct cggtgctttc cgcgcctttt tccgccacgg tgtacagcag ttgctgtttt
     3181 ttcaggtttt cgatcagcac gcggtcgttg cctggcatca ggttaacttc gcagataccc
     3241 cagtcgctgt cggtgctttc cagcaggtga tgggtataca gcgcctggtg cgcgcggtga
     3301 aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgctc ccatgacggg
     3361 cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag aatccgtctt
     3421 cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc ggtgggtatt
     3481 aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg gccaggcctt
     3541 tcagacgacc aattgcggag taacctgggt tggttttctt cttcaggtcg tccagcatct
     3601 gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct ttacgacggt
     3661 gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg ttcaggtgcg
     3721 ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga tgggtgaagt
     3781 aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca cgcacgccgt
     3841 aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca accatccact
     3901 gcatatcttc aatggtggaa acaatgcgcg gcaggccaag gatcgggcgc ggcggatcgt
     3961 ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc gctttcagga
     4021 agacggcaaa gttttcgcgc agcttcgctt tatcgatatc tttgtacagc tccaggtgct
     4081 tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca atgatgttac
     4141 gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgaacagca gcctgagcaa
     4201 tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc atttcgaatg
     4261 cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc acgtattcga
     4321 ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg cgaatgccgc
     4381 actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca tagttgccag
     4441 tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac accaggcctg
     4501 cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact tcgccgttcg
     4561 ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca tcagctaaag
     4621 aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt cctctttcac
     4681 atagtgggtt ccacagcaaa gagacgcgga accctggagt tgtttaggta tccggatgaa
     4741 caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt ctaccttttg
     4801 gtaagacatg tcaacccgaa ttctgaaatt ggttaaccac atcacaagaa tttcactctc
     4861 atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca gaggatgtga
     4921 cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat aacaacggtt
     4981 caaccaggta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg tattcggcat
     5041 tggcctgatg ctggtactga caaagaccgc aggttcagac acattcgctg cattagcaat
     5101 gcctcttaga taattttgac ctgtcagtaa aagacacgtt gaaaacctgg ggtctgctgg
     5161 agctggtcaa ctccgtggtt gggctgatta ttagcaaggt agcgtaaaag aacaccagag
     5221 cctgccaatg gcaggctctg actgatgaca aagtctaaaa atgtgcccgg acagtcccct
     5281 cgcccctccg gggagagggt tagggtgagg ggaacagacc agcaaagtgc gctttgttta
     5341 tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt gcaggttttt
     5401 agcttcaggt aatattgcgt aactgcatta gcaatgccct gtgatttctt tattgataaa
     5461 caaaagtcac gccaataatc gattgcacat tccctgcagt cacctgccct ccggtacgtg
     5521 cgtaatttgc cgttaatccc agacttaccg ccgaagtccc tactgctcct aacgataccg
     5581 tgttattcgc tggaataatc gtaccgttgc gcgtcaactg tacgccgacg ccctgcgctg
     5641 gtgaaaacga cgcggtattg gtgaaaatcg agttgcccgc atctacggtt gtgccggaga
     5701 ggtaataccc cagattttgg cttttcgcac aataaacggt aagaggaatt ggcactgaac
     5761 cagggtagtc cggcagagta acggtgacat cacgagcaga aacatcgcag ccgccagtag
     5821 gcaccaccac atcattattg gcgtaaatat tccacacaaa ctgaaaatca tcgctgttat
     5881 agttgttggt ctgtcgcaaa ataagcacgg caattaatga gccagcttta atcgccaccc
     5941 cgcccgcact gctcacaggc gtcaaataaa gcgccaccgg ccacggctta tccgttctcg
     6001 aattataaac aacgcgcggc gtttcgctgg tggtaggaaa tggatcgcta ctgccactat
     6061 attttacggt cccggaaaaa ttagataaca cgctgccata agccgagcct cgttgcagtg
     6121 tgacatagtc tgtaatggtt tccggataat cgttatggca aaagatttgc gtcgaaagat
     6181 ccacgaccag gttttgcccc acattcacgg cgggcgcaag gtttacataa acattggcgc
     6241 tgccaccgcc aatagggata gcggtaccat tggcggtttt acaggcgaat gaccaggcat
     6301 ttaccgacca gcccatcagc agtacagcaa acagggtaat aactcgtttc attacaatca
     6361 tctcttcggg ttcagctgta ggtataggtg atgctaatca ctgcctgaat ggttccctga
     6421 gtggctccgc catttaccgt caatgctctg acctgtaacg ggaagtgcgc tgattgtgag
     6481 gaatcatcca cctgaactgt tttggttgcg ccagtattca atgtgttgcc actgtcatcc
     6541 tgtagctcta actggatgtt ttgcgcggtc ccctggtttt tataatatcc ggtactgtcg
     6601 gctgccccgc tgaagctggc ggtgaccctc gacgttccca ccggacaatt agtcaactca
     6661 agcgcaacat catgccaggc cgatgccgcc ccggcagaca tcagactgaa agaataaaga
     6721 tcgccgagat caaccgtggc attggtggtg gaaaccgtac acggtttggc gacgacctta
     6781 ccgttcaccg tgatggtgac atcggctgcc tgtatcgtcg cacttgcgag cgccaatatt
     6841 gccgccaata aatacccacg tttgcgccat ttcatgagca tctccagtta ctgatattca
     6901 agagtgaagg tagccgtggc attgatatgc cccgcagtga caggcacctg tgtcgccatt
     6961 agccgggcgt aaaaattcaa cgtatttggt ttacccggcg tcagggtcgt ccacgaaatc
     7021 gcggacgatg gagcattaag gggtatttga ttttgctgct cattcagaag ctgtattccc
     7081 agtcccgaag ccgctgacac cgtattttca agtgcaagca gattggcatt gtggctatct
     7141 gcaacgccgg taaacccaac ttttacggca gaaacggcat taccacaggg tgacagcaaa
     7201 atacgaaatg gaacaacagg agtcgtcgcg ccaatgttgt taaattgctt cgccgcgttt
     7261 tccatcagat caacagtaaa attggttgat tcagcggcca cactacagcc gttatctctg
     7321 acatagccgc ggatagtaat cgtgctatcc gcagccaaag cgtgactcac cgccagccac
     7381 aaaaaagcgc acagaagata aaaaggtttg tttctcatca cgccccctta acgacattca
     7441 gctgatagct gggttaataa ctgctgctga ctctctggtg gcagttgata attggcgaca
     7501 cagtgagcat tttcctcttc tccccatttc acctgaactt ttcccgctaa aggcattccg
     7561 ctgaagtaaa cctgaccatt atccgcaaca atgccgctac tctggctact ctctgatgtc
     7621 accatcgccc caaacggcag cggcttatta ttgtgggtta gcgtcatgag cagttttatc
     7681 ccaacgcgcg ctttaaactc tgctcgcacg atcgccccac gagtgggaac aacgttagcg
     7741 accgcgttat ctaaatcgac gttatcagcc agggtattgg tatccagcgc cactctgttt
     7801 tccctatatt cagtggcata aggcagcacg gcataaccgc gccagtcggt acgcaccccc
     7861 gtctggtttt cgacttttgc atcttttgcg ccaggcgctt taacaagcac caccgtatcg
     7921 tttaacggct gccccagcgt tacgccattg gcatgagcca ataccccacc gctgactccg
     7981 taatagagct gcttaatatc atcgctatgg ctgtaaccga tattggcatt gccgtaacca
     8041 ccgcgataat tcagcgtggc gtagcctgtg cttccgctat taccatcgcc tcccccggca
     8101 tagccggttt gcacgctata gctgaggttg ttgtcttcca gcaacgtacc gtatacacca
     8161 gccagattgg tcatccgacc gttgagatcg tgtgacatgc tgtagctggc actggcatgt
     8221 cgccactgag atttactgtc agaacgcagc cagtggctga aaggaatatt gacgttaagc
     8281 gctaacatct gatcacgtcc tttttgccag gcgtttttcg tcaggctata gctgagcgtc
     8341 cagttgatat cttcgaacgc agtatttaat ccagcctgga attgctcatc gacattactc
     8401 gttccccaat aagtttgatg gctaccactc aaatacagtg ttgatgtgcg cccgagttgc
     8461 tgagtaacgg tgagctgtaa tttcccgcgt ttgttataag cgaggttgta atagtcggtg
     8521 aatttcggct taacctgaat aactccatcc tgtgtttcga tgttgtagcc attcattcga
     8581 ctgtatgttg tatcagcgaa attaaaatat ccgctggtcg aataacggta acccactaac
     8641 tgaatattcg tgcctgattc attgagcgat ttgttataga gaaaacgcac cgattgtccg
     8701 tcatgctgac tgtcatcggg aagtgtggaa ttagcctgcg tcatatccac agacagagcg
     8761 cccagtgccc ccatgttttt cccgataccg aaattaaaag cacgataacg atccgccagt
     8821 tgcgttccac catatattgt ccagccagcc ggaaggccgt ggagtaatgt actctggaaa
     8881 aagcggggtt tttcctgctg cgcatttcca ctacggtatt ctcctgccgt aatggaataa
     8941 cgagtatgcc cttcacgttg caaaagcggg actgacgaat agggtacggt aaaaatctgc
     9001 gtgctgccgt cagcctcttt gatcgttacc tgcaagtcac cactattacc tgcggcatag
     9061 atatcgttga tggtaaaagg ccccggtggc accgtactat tataaatgtc atacccattt
     9121 tgtttaatag tgacctgtgc agtaccacga gcaataccgt ggatcaccgg ggcaaatcct
     9181 ctttggctat cgggtaacat attgtcatct gaggccaatt gtgcgccgcg aaagttaata
     9241 ccatcgaaaa tatcgccctg agtataacca tcacccagcg tcagccggga acgtaacggt
     9301 attatgtctc gctcaagcca ggtattgata tgctgccatt tatttttgct acctgatgat
     9361 ctgtcgctac tgttataact ccaggtggta ttgtcgcgta aacgccacgc accaatattt
     9421 aacccactct gtaggtttaa atatgcataa tggctgttac ccccaatccg attctgtaca
     9481 ctatttccgc tgaaattata attgagcaat ccggcattaa taccgggatc ccataactca
     9541 ggaggaatat aaccacgcgc gcgattactc ataaatgcct gagggatcgt caggttcagt
     9601 cgctgctgac caacatccag atgcgcagta gcgtcctgga ccattgtggt taatggcaca
     9661 caggcatcat ccgccagcag attcataccg gcgacagaag ccgtattcag ccccatactg
     9721 gcgagttgcg cgcgtgtcag gcagggaaca atcccttgtt cactgtcgcc cgtattaaat
     9781 gtgacatcac gcgttgccat ataaccatta ttcaaataga tatcgacgcg atacgtccct
     9841 ggcggtaatt cttgcccatt ttcaaaacgc gataaatcgg ccacagcctg gggatcatcc
     9901 gctaaaaaac gcgggttaaa atagagttcg gcagatgaca aaggtgcctg tgcggcaaaa
     9961 gcacaggcaa caaagagccg gacaaaaaaa ccagccaaac gatgcttacg aatatgcaag
    10021 cattgtgtgt ttcgctggta aagtcttaaa ttcagatatg acattattcc tccggtttaa
    10081 agtcacccgg cagtcaattc tttttattca ggcgaaaaat tccccctgcg ttattccatt
    10141 acgcccgtca ttttgggggt aagtgcacca taatcattta ttgttcggta agtaatattg
    10201 cttcccgcat cagaaggcaa tttaaccgtg ctttcgccca ttggaggcac caaggcattt
    10261 tcaagaaccc gggttccggc attcaactct gttaccgtca ggtaataggg tgtcgggtta
    10321 atcagcgtca gagaattcgc gctacgacga aatcttaatt tttctgcggc ctgatcgggt
    10381 ggcaacgcta atttagccgg gcgatagtac agtttaatgc ggctgataat tgcgagctgt
    10441 agcgtattct cagtcaattt tgatttatcc attgacggaa tcgctttaac attcatccag
    10501 aataaacttt cccgatcctg tggcaattgg ttatttgttg catcaagaat acgcaaggta
    10561 ttctcttttt ttcccttcat cgcaaacaga ggaggcgtca cgataaaacg accatccttt
    10621 acaccatcgg cattttccac ccatgattga attaaatagg tactattttc atcattattt
    10681 gtcacggcaa gttgcacttg tttttgccct gccggataaa ttacgcgagt cgcacctaag
    10741 gccactcccg cttcagcgcg tccggcaacc atcattgcca taaacatcag gatacctgcc
    10801 agcaagcaga atgttatttc ctgcgatttc cttacattga cgtttttatt actcactgtc
    10861 ctgttcctgt tatcacatta tctgctgaac aattactgat aggttaaaga gaaccaggcc
    10921 tgggcattgg cgatgccgcc agtaacccga cgcccggtag cacgatattt ggcgatgaaa
    10981 tgtagcgaag tagagcctga gtaaagccgt ttccagtttg ctggaggacg attaatcggt
    11041 acgaggtttc cttcatcatc aaacaacgct acgccaatat tggtggctat ccctggcccc
    11101 tctcccacgg aaagcacatc cggattttta ccatccgcga caccgtgaaa cgccaccccc
    11161 acacgttcac tcaccaccgt gctacattcc cgtaaatgaa taacaaaagg caccggtgcg
    11221 ctatcttccc ctgccgcatg aaaccggtta ctgctgattt gccccatatt gaccgtcatt
    11281 tgtttatcac cggcttcaat ccggcaagtt tccgcaataa tgacgccctg aaattgcata
    11341 tttccgccgg gcagcgtggt attccattta tttccggcca gagcaaacat tggcagcaac
    11401 gacgctaaca gaaataatct cttccttttc atcgttattt ttatcgcaca agggtgggca
    11461 tccctgcccg taatgacgtc cttgaacctg ggtaggttat tgatactgaa ccttgaaggt
    11521 tgcatccgca ttagctgcac ccggggttgc ctcgccgatt gcataataac gcgcctggaa
    11581 cggaatggtg ttggtaccgt tattcagggt tgtttgtgca ctaaatgtcg caccgtccag
    11641 cgtcagagca ttgcctgttc tgtccaggat ctgcacgcca acgtttgttg cactacctgc
    11701 agcagaactc tgcagagcca gtacatcagt acgcgtagca tcaattgccg tacctaagaa
    11761 ggcaacagcg gcttttgtgg caacagtggt atcgcaatca ttcaactgaa tgttaaaacc
    11821 aacggcagag ctggttgctc cagcctgctt caggctagcg gtacgaacct gtcccaactg
    11881 aacggtttga tcaacagagc ctgcatcaac tgcgcaagcg gcgttaacaa cttccccttt
    11941 aaagtgaacg gtcccaccat ttaccgtcgt agtatcggcc agagccgctg cggaactgag
    12001 ggacagagcc gacagaacaa cgattgccag agttttaatt ttcatgctgc tttcctttaa
    12061 aaaaactatt tctaaatcga catgggcagt cgttctgtac actttgtttt gtcaacgagt
    12121 ttgactgcca acactgcaca gttttccccc aaaagatgaa acatttgggg cca
//
LOCUS       DAZFWL020000136        12110 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFWL020000136 DAZFWL020000000
VERSION     DAZFWL020000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.136"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            222..1454
                     /gene="mdtM"
                     /locus_tag="ACHKNP_RS20005"
                     /old_locus_tag="pgaptmp_004001"
     CDS             222..1454
                     /gene="mdtM"
                     /locus_tag="ACHKNP_RS20005"
                     /old_locus_tag="pgaptmp_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HGU4706077.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVHQHQAAELAEER"
     gene            1495..2775
                     /gene="yjiN"
                     /locus_tag="ACHKNP_RS20010"
                     /old_locus_tag="pgaptmp_004002"
     CDS             1495..2775
                     /gene="yjiN"
                     /locus_tag="ACHKNP_RS20010"
                     /old_locus_tag="pgaptmp_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HGU4706078.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            2891..4042
                     /gene="yjiM"
                     /locus_tag="ACHKNP_RS20015"
                     /old_locus_tag="pgaptmp_004003"
     CDS             2891..4042
                     /gene="yjiM"
                     /locus_tag="ACHKNP_RS20015"
                     /old_locus_tag="pgaptmp_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HGU4706079.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            4052..4819
                     /gene="yjiL"
                     /locus_tag="ACHKNP_RS20020"
                     /old_locus_tag="pgaptmp_004004"
     CDS             4052..4819
                     /gene="yjiL"
                     /locus_tag="ACHKNP_RS20020"
                     /old_locus_tag="pgaptmp_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418754.2"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HGU4706080.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEAISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            4816..5073
                     /gene="ytiA"
                     /locus_tag="ACHKNP_RS20025"
                     /old_locus_tag="pgaptmp_004005"
     CDS             4816..5073
                     /gene="ytiA"
                     /locus_tag="ACHKNP_RS20025"
                     /old_locus_tag="pgaptmp_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HGU4706081.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEMQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            5138..5998
                     /gene="yjiK"
                     /locus_tag="ACHKNP_RS20030"
                     /old_locus_tag="pgaptmp_004006"
                     /pseudo
     CDS             5138..5998
                     /gene="yjiK"
                     /locus_tag="ACHKNP_RS20030"
                     /old_locus_tag="pgaptmp_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
     gene            6066..7244
                     /gene="yjiJ"
                     /locus_tag="ACHKNP_RS20035"
                     /old_locus_tag="pgaptmp_004007"
     CDS             6066..7244
                     /gene="yjiJ"
                     /locus_tag="ACHKNP_RS20035"
                     /old_locus_tag="pgaptmp_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4706082.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(7257..7811)
                     /gene="kptA"
                     /locus_tag="ACHKNP_RS20040"
                     /old_locus_tag="pgaptmp_004008"
     CDS             complement(7257..7811)
                     /gene="kptA"
                     /locus_tag="ACHKNP_RS20040"
                     /old_locus_tag="pgaptmp_004008"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HGU4706083.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRTLLDTVVATSDKKRFSYSSDGRCIRAVQGHLTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQTENGVWLTSTVAVEFLEW"
     gene            8061..8744
                     /gene="yjiH"
                     /locus_tag="ACHKNP_RS20045"
                     /old_locus_tag="pgaptmp_004009"
     CDS             8061..8744
                     /gene="yjiH"
                     /locus_tag="ACHKNP_RS20045"
                     /old_locus_tag="pgaptmp_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGU4706084.1"
                     /translation="MTQQGDAVAGDLATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            8741..9202
                     /gene="yjiG"
                     /locus_tag="ACHKNP_RS20050"
                     /old_locus_tag="pgaptmp_004010"
     CDS             8741..9202
                     /gene="yjiG"
                     /locus_tag="ACHKNP_RS20050"
                     /old_locus_tag="pgaptmp_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HGU4706085.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            9215..10387
                     /gene="iadA"
                     /locus_tag="ACHKNP_RS20055"
                     /old_locus_tag="pgaptmp_004011"
     CDS             9215..10387
                     /gene="iadA"
                     /locus_tag="ACHKNP_RS20055"
                     /old_locus_tag="pgaptmp_004011"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU4706086.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILFPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            10452..11364
                     /gene="hypT"
                     /locus_tag="ACHKNP_RS20060"
                     /old_locus_tag="pgaptmp_004012"
                     /gene_synonym="qseD"
                     /pseudo
     CDS             10452..11364
                     /gene="hypT"
                     /locus_tag="ACHKNP_RS20060"
                     /old_locus_tag="pgaptmp_004012"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
     gene            complement(11357..11749)
                     /gene="iraD"
                     /locus_tag="ACHKNP_RS20065"
                     /old_locus_tag="pgaptmp_004013"
     CDS             complement(11357..11749)
                     /gene="iraD"
                     /locus_tag="ACHKNP_RS20065"
                     /old_locus_tag="pgaptmp_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HGU4706087.1"
                     /translation="MMRQSRQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSSLPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(11746..11829)
                     /gene="idlP"
                     /locus_tag="ACHKNP_RS20070"
                     /old_locus_tag="pgaptmp_004014"
     CDS             complement(11746..11829)
                     /gene="idlP"
                     /locus_tag="ACHKNP_RS20070"
                     /old_locus_tag="pgaptmp_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HGU4706088.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(11945..12088)
                     /gene="ytiD"
                     /locus_tag="ACHKNP_RS20075"
                     /old_locus_tag="pgaptmp_004015"
     CDS             complement(11945..12088)
                     /gene="ytiD"
                     /locus_tag="ACHKNP_RS20075"
                     /old_locus_tag="pgaptmp_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HGU4706089.1"
                     /translation="MADYAEINNFTPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
BASE COUNT         2723 a         3055 c         3136 g         3196 t
ORIGIN      
        1 aaccatcaat tcaagaggtg taatgtgctg atgactattt gtaatcgtta tacattctga
       61 cccgaactca gaaggtattt ctctgtctgt ataagcacag gcagtgtggt tgattacatg
      121 aaattcagta catttgtcct tctcgcctac ctttcgtcat taccgacggt attgaatttc
      181 gttttccccg ttggggttct ccggacaagg agttgtttgt tatgccacgt ttttttgccc
      241 gccatgccgc cacgctgttt ttcccgatgg cgttgatttt gtatgatttt gccgcgtacc
      301 tgtcgacgga cctgattcag ccggggatca ttaatgtggt ccgtgatttt aatgccgatg
      361 tcagtctggc cccggcagcc gtcagtctct atctcgcggg cggtatggcg ttacagtggc
      421 tgctggggcc gctttccgac aggattggcc gcaggccggt gctgattacc ggggcgctaa
      481 tatttaccct tgcctgcgct gcgacaatgt tcacaacgtc tatgacacag tttcttatcg
      541 cgcgtgcaat tcagggcacc agtatctgtt ttattgctac cgttggttat gtcacggtgc
      601 aggaggcgtt cggacagaca aaagggatca agttgatggc gattatcacc tccatcgtac
      661 tgattgcgcc gattattggc ccgctttccg gcgcagctct gatgcacttt gtgcactgga
      721 aagtcctttt tgccatcatt gcggttatgg gttttatctc atttgttggc ttactgctgg
      781 cgatgccaga gacggtgaag cgcggcgcgg ttccatttag cgccaaaagc gtcttgcgcg
      841 attttcgtaa tgtcttttgc aatcggctgt tcctctttgg cgcagcaacc atctctttaa
      901 gctatatccc aatgatgagt tgggtggctg tctcgccggt gatcctgatt gacgcaggcg
      961 gcttaacaac ttcgcagttc gcctggacac aggttccggt gttcggcgcg gtgattgtcg
     1021 cgaatgccat cgtggcgcgt ttcgttaaag atccgaccga accgcgcttt atctggcgtg
     1081 ccgtacccat tcaactggtc ggcctcgcac tgttgattat cggcaatctg ctgtcaccgc
     1141 acgtctggct gtggtcggtg ctgggcacca gtctgtatgc tttcgggatt ggtttaatct
     1201 tcccgacctt attccgcttt acactttttt ccaataactt accgaaaggg accgtctccg
     1261 catcgctgaa tatggtgatc ctgatggtga tgtctgtctc ggtcgaaatc ggccgctggc
     1321 tgtggtttaa cggcggtcgc ttgccgtttc atctgttagc cgtcgtagcg ggagttatcg
     1381 tcgttttcac cctggcgggg ttgctcaatc gcgtgcacca acatcaggct gccgagctgg
     1441 cggaggagcg gtgatttttg cgcgatcctg ccgtcaggct ctattcttaa cgttatgaat
     1501 aaactcattg aactcagacg cgccaaaatg ttggcgctct ctttactgct tatcgccgct
     1561 gctacctttg tcgttacgct gtttttgcca cccaattttt gggtgagcgg cgtgaaggca
     1621 attgctgaag cggcgatggt cggcgcgctg gcggactggt ttgcggtggt ggcgctgttt
     1681 cgccgcgtgc caattccgat catttctcgt catacggcga ttatcccgcg taataaagac
     1741 cggattggcg aaaatctcgg tcagttcgtg caggaaaaat ttctcgatac ccagtcgctg
     1801 gtggcattga ttcgacgtca cgaaccggcg ttgctaattg gtaactggtt tagccagcca
     1861 gaaaacgccc gccgcgttgg tcagcatctg ttgcagatca tgagtggttt tcttgaactg
     1921 accgatgatg cacgtattca gcgcctgctt aagcgcgcgg tccatcgggc gattgataag
     1981 gtcgatcttt ccggaaccag tgcgttgatg ctggagagta tgaccaaaaa cgatcgtcat
     2041 caggtgctgc tcgatacgct gatcgcacag ttgattgctc ttctccagcg cgataaatcg
     2101 cgcaagttta tcgcccaaca aattgttcgc tggctggaga gtgagcatcc actgaaagcc
     2161 aaaattctcc ccaccgaatg gctgggcgaa catagcgcgg agttggtttc tgacgcggtg
     2221 aattctttgc ttgatgatat cagccgcgat cgtgcgcatc agatccgcca tgcatttgat
     2281 cgcgccactt ttgccctgat cgacaagttg aaaaacgatc cggaaatggc agcgcgagct
     2341 gatgccgtaa aaagctatct gaaagaggat gaagccttta accgctatct cagtgaattg
     2401 tggggggatt tacgggaatg gctgaaagcg gatatcaaca gtgaagattc tcgtgtgaaa
     2461 gaacgtatcg cgcgggcggg tcaatggttt ggcgaaacgt taattgccga tgatgccttg
     2521 cgggcgtcgt taaatggtca tctggaacaa gccgcgcacc gcgtcgcacc tgagttttcc
     2581 gcattcctga cgcgccacat cagcgatacg gtaaaaagct gggatgcccg ggatatgtcg
     2641 cggcaaatag agttaaatat cggcaaagat ctgcagttta tccgtgtcaa cggtacgctg
     2701 gttggcggtt gtattgggct aattttatat ttgctgtcac agctcccggc cttgttcccc
     2761 ctcggcaatt tttagaaatc catgaaaaac taagtggcta accgcgagag agatcaaata
     2821 aaccacacta aaagaatggt aataatgtgg cctcttttca ttatctcccg tggtacgggg
     2881 aaggaaaatc atgtcacttg tcaccgatct acccgccatt ttcgatcagt tctctgaagc
     2941 tcgccagaaa ggctttctca ccgtcatgga tctcaaggag cgcggcattc cgctggttgg
     3001 cacttactgc acttttatgc cgcaagagat cccgatggca gccggtgcgg ttgtggtttc
     3061 gctctgttcc acctctgatg aaaccattga agaagcggag aaagatctgc cgcgcaacct
     3121 ctgcccgctg attaaaagca gctacggctt cggtaaaacc gataaatgcc cctacttcta
     3181 cttttctgat ctggtggtcg gtgaaaccac ctgcgacggc aaaaagaaaa tgtatgaata
     3241 catggcggag tttaagcctg ttcatgtgat gcagctgcca aatagcgtta aagacgatgc
     3301 ctcgcgtgcg ttatggaaag ccgagatgct gcgcttacaa aaagcggtgg aagaacgttt
     3361 tgggcacgaa attagcgaag atgctctgcg cgatgccatt gctctgaaaa accgcgaacg
     3421 tcgcgcactg gctaattttt atcatcttgg gcagttaaat cctccggcgc ttagcggcag
     3481 cgacattctg aaagtggttt acggcgcaac cttccggttc gataaagagg cgttgatcaa
     3541 tgaactggac gcgatgaccg cccgcgttcg tcagcagtgg gaagaaggcc agcgactgga
     3601 cccgcgtcca cgcattttaa tcaccggctg cccgattggc ggcgcagcag aaaaagtggt
     3661 gcgcgcgatt gaagagaatg gcggctgggt tgtcggttat gaaaactgca ccggggcgaa
     3721 agcgaccgag caatgcgtgg cagaaacggg cgatgtctac gacgcgctgg cggataaata
     3781 tctggcgatt ggctgctcct gtgtttcgcc gaacgatcag cgcctgcaaa tgctcagcca
     3841 gatggtggaa gaatatcagg tcgatggcgt agttgatgtg attttgcagg cgtgccatac
     3901 ctacgcggtg gaatcgctgg cgattaaacg tcatgtgcgt cagcagcaca acattcctta
     3961 tatcgctatt gaaacagact actccacctc agatgtcggg cagctcagta cccgtgtcgc
     4021 ggcctttatt gagatgctgt aaggagtggc agtgacatat tcgattggca ttgattctgg
     4081 ttctaccgcc accaaaggga tcttactggc agacggcgtg attacgcgcc gtttcctcgt
     4141 tccaacaccc tttcgtccgg caacagcaat tactgaagcc tgggaaactc tgcgcgaagg
     4201 gttagagaca acgccgtttc tgacgctcac cggctatgga cggcaactgg tggattttgc
     4261 cgataaacaa gtgacggaaa tctcctgtca tgggctgggc gcacggtttc ttgcgccagc
     4321 aacgcgcgcg gtaatcgaca tcggtggtca ggacagcaaa gtgattcagc ttgatgatga
     4381 cggtaacctg tgcgatttcc tgatgaatga caaatgcgcg gcgggcaccg ggcgtttcct
     4441 ggaggtgatc tcgcgcacgc ttggcaccag cgtcgagcaa ctcgacagca ttaccgaaaa
     4501 tgtcacgccg cacgccatca cgagtatgtg cacagtgttt gctgaatcag aagcgatcag
     4561 cctgcgctca gcgggcgtcg cgccagaagc gattctcgcc ggagtgatta acgcgatggc
     4621 gcggaggagt gccaatttca ttgctcgtct ctcctgtgaa gcgccgattc tgtttactgg
     4681 tggcgttagt cattgccaga cgtttgcccg gatgctggaa tctcatctgg gaatgccggt
     4741 aaatacccat cctgatgcgc aatttgctgg cgcaattggc gcggcggtaa ttggtcaacg
     4801 agtgaggaca cgccgatgaa agagttttta tttttatttc actccacagt cggcgtcata
     4861 caaacccgca aagcgttgca ggcagcgggc atgacttttc gcgtcagcga tattccccgt
     4921 gatttacgcg gcggctgcgg gttatgcatt tggctgacct gtccccccgg cgaggaaatg
     4981 caatgggtga tccctgggct gactgagtca atttattgtc agcaggatgg tgtctggcgc
     5041 tgcattgcac attaccgggt ttctccgcgt taaggattca ttaaggtcgc tacgtatact
     5101 tctcgatatc ttattgattt gccttaaagg gattaccgtg acaaaaagta tttcgttaag
     5161 taaacgcatc tccgttattg tgattctttt tgccattgtg gcagtgtgta ctttcttcgt
     5221 gcaatcttgc gcccgtaaaa gcaatcatgc agcataattt cagaattacc acgccaccat
     5281 tgacggcaaa gaaattgcag gcataacgaa taatatctct tcgctgacct ggtcggcaca
     5341 aagcaatact ctttttagca ctatcaacaa acctgccgcc attgttgaaa tgaccaccaa
     5401 tggtgatttt atccgcacaa ttcctctgga ttttgttaag gatctcgaga cgatcgaata
     5461 cattggcgat aatcagtttg tcattagcga tgagcgcgat tacgccattt atgtgatttc
     5521 actgacccca aactcggaag taaaaatcct caaaaaaata aaaatcccgc tgcaagactc
     5581 cccaaccaat tgcggttttg aagggttggc ttattctcgc caggatcaca ctttctggtt
     5641 ttttaaagag aaaaatccga ttgaggtcta caaagttaac gggctgttaa gtagcaatga
     5701 attacacatc agcaaagacg aggcactaca acggcaattt actttagatg atgtgtccgg
     5761 cgcagaattt aaccagcaaa aaaatacgct tctggtgctg tctcatgaat ctcgcgcact
     5821 ccaggaagta accctggttg gcgaggttat cggtgaaata tcactaacca aaggtagtcg
     5881 aggtttgtcg cataacatca aacaggccga agggattgcg atggatgcct ctggtaatat
     5941 ttatatcgtt agcgaaccaa atcgttttta tcgctttacc cctaaatcat cgcattaaca
     6001 ggctggcata tttcaccttt ttgttattaa cttgttaaca acaaaaatga catacaaggt
     6061 taaacatgcc ttcgtcctcg catcccgtag aacgcttttc tttcagcacc gcgcttttcg
     6121 ggatgctggt tctgacctta ggtatgggat taggtcgctt cctttatacg cctatgttgc
     6181 ccgtcatgat ggcggaaggg gcgttttcat ttagccagct ctcgtggatt gccagcggca
     6241 actatgcggg gtatctggct ggcagtctgc tattttcgtt tggcgcattt caccagccgt
     6301 cgcgcctgcg cccatttctg ttggtttctg ccctggcgag cgggttgttg atcctcgcaa
     6361 tggcatggtt gccgccattt attctggtgt tattgattcg cttcctggcg ggtgtcgcca
     6421 gcgccggtat gctgattttt ggttcgacgc tgattatgca gcacacgcgc catccttttg
     6481 tgctggcagc tttgttttct ggcgttggca ttggcattgc actgggcaac gaatatgttc
     6541 tggcaggcct gcattttgcc ttatcctcgc aaacgttatg gcaaggcgcc ggggcgcttt
     6601 ctggcatgat gctgattgca cttacgcttt taatgccctc gaaaaaacac gccatcgcac
     6661 caatgccatt ggcaaaaacg gagcaacaga taatgagctg gtggttactg gctattctgt
     6721 acggcctggc gggatttggt tatatcatcg tcgcgactta cctgccgctc atggcgaaag
     6781 atgcaggctc accattgtta accgcccatc tctggacgtt agtcggctta tcaattgtgc
     6841 caggctgctt tggctggcta tgggcagcaa aacgttgggg ggctttgcct tgcctgacgg
     6901 cgaatttgct ggtgcaggct atcagtgtgc tgcttactct cgccagcgac tctcctctct
     6961 tgcttatcat tagtagtatc ggttttggcg gtacctttat gggcacgaca tctctggtga
     7021 tgactatcgc ccgccaactg agcgttccgg gaaatctcaa tcttttaggc tttgtgacgc
     7081 tcatttacgg cattggacaa atcctcggcc cggcgctgac cagtatgctc ggcaatggaa
     7141 catcggcgct tgccggcgcc actctctgcg gcgcggcggc gttgtttatt gctgcgctta
     7201 tcagcacagt acaattattt aagctgcaag tggtcacttc atgaggagtg tttcccttac
     7261 cactcaagaa attcgaccgc aaccgttgat gtcagccaga ccccgttttc cgtttgccag
     7321 aaagggatgc ctcgtttcgc catttcctgt gctttgacgg ttaagataac cggcgatccg
     7381 tgtcttgctc ccactttgcg tgccgttgct tcatcagcag agagatgtac gtagtgacgc
     7441 tcacctgcaa tcagcccctg tttttttatc tcgtcaagga aacggcttgc cgtaccgtgg
     7501 taaagaaact gtggcggtgt tttttcggca aaggaaatag cgacctgcga agtcaaatgc
     7561 ccctgaaccg cgcggatgca cctaccatca ctggaataac taaaacgctt tttatcactg
     7621 gtggcgacca cggtatccag caatgtacgg gttaatcgct tgcccgcttt ttgggcacaa
     7681 agtatgagct tatcgatatc cgcccatcct tcacggtcaa gtacaatacc aatcgcctcc
     7741 ggtttgtgtc ggaggacaaa acttaaaaac ttgctcgtat ccgccagctc tttttcgttg
     7801 tactttgcca tctcgttttc gtccctggat tttctgcttt tatttactga tgtcattgtc
     7861 actgggttta tgaattttct gcaactcaat cctgcgcaga gccttttcca cacagcgtaa
     7921 tttttccgct atgcaaaaaa cgcatagcca gaaacatact tgcattttcc ccatacccac
     7981 atggccgata aggtttttca gctccaggaa ttttcctggc tcaagcaaca cacaaacaca
     8041 aaaaaacaat ggggattgtt atgacacaac aaggggatgc tgtagccggg gatctggcga
     8101 cagaaaaagt gggcattaaa gggtatctgg ctttttttct caccataatc tttttttccg
     8161 gtgttttttc cgggacagac agctggtggc gcgtttttga tttttccgtt ctgaacggat
     8221 cgtttgggca actgcctggt gcgaacggag ccaccacctc atttcgcggg gcgggtggcg
     8281 cgggggctaa agatggcttt ctctttgcgc tggagctggc accgtcagtc attctctctc
     8341 tggggattat ttcaattacc gatggtcttg gtggattacg cgccgcgcag cagttaatga
     8401 cacctgtgct aaaaccgttg ttgggcattc cagggatctg ctcgctggcg ctgatcgcta
     8461 acctgcaaaa cactgatgcc gccgccggta tgaccaaaga gctggcgcag gaaggggaaa
     8521 ttaccgaacg cgataaagtg attttcgccg catatcaaac cagcggtagc gccatcatca
     8581 ccaactactt ctcttccggc gtcgcggtgt tcgcctttct gggcacgtcc gtaatcgtac
     8641 ctttagccgt catcctggtg tttaaatttg tcggagccaa cattttgcgc gtctggctta
     8701 acttcgaaga acgtcgcaac ccaacgcaag gagcgcaagc atgacaactc aggtccgcaa
     8761 aaacgtcatg gatatgttta tcgatggcgc gcgccgtggc tttactatcg ccacgaccaa
     8821 cctgctgcca aacgtggtga tggcgtttgt catcattcag gcgctgaaaa ttaccggcct
     8881 gctcgactgg gtggggcata tctgtgaacc cgtaatggcg ctgtggggat tacccggtga
     8941 agcggcaacc gtccttttag ccgcattaat gagtatgggt ggcgcagtcg gtgttgccgc
     9001 cagtctggca accgctggcg cattaaccgg tcacgatgtc accgttttac tgcccgcgat
     9061 gtatttaatg ggcaacccgg tgcaaaatgt cggtcgctgc ctggggacag ccgaagtgaa
     9121 tgccaaatat tacccgcata tcattacggt ctgcgtgatt aacgcattgc tgtcgatctg
     9181 ggttatgcag cttattgttt aacaaggagt taccatgatt gattataccg cagccggttt
     9241 taccctgctg cagggagcgc atttgtatgc gccggaagat cggggaattt gcgatgtcct
     9301 cgtcgctaac ggcaaaatta tcgccgttgc cagcaatatc ccttctgaca ttgtaccgga
     9361 ctgcacggtt gtcgatctca gcgggcagat cctctttcct ggttttattg atcaacacgt
     9421 ccatttgatt ggtggtggcg gtgaagcagg tcccacgacg cgcacgccag aagtggcgct
     9481 aagtcgcctg acggaagcgg gcgtcacgtc agtggttggt ctgctgggca ccgactctat
     9541 ctctcgccac ccggaatccc tgctcgccaa gacccgtgcg ctcaatgaag aaggcatcag
     9601 cgcctggatg ctgaccggcg cttatcatgt cccttcccgc accattacag gttccgtgga
     9661 aaaagacgtg gcgattatcg atcgtgtgat tggcgtgaaa tgcgccatct ctgatcaccg
     9721 ttctgccgca ccggacgttt atcacctggc taatatggcg gcggaatccc gcgttggcgg
     9781 tttgctcggc ggtaaacctg gcgtcaccgt gttccacatg ggcgacagca aaaaggcgtt
     9841 acagcctgtc tatgacctgc tggaaaactg cgatgtgccg atcagcaagt tgctgccgac
     9901 ccacgttaac cgcaacgttc cgttgtttga gcaggcgctg gagttcgcgc gcaaaggcgg
     9961 cactatcgat atcaccagca gcattgacga accggtcgcc cctgccgaag gtattgcccg
    10021 cgccgttcag gcgggtattc cgctggcacg cgtcaccctc agctccgacg gcaacggtag
    10081 ccagccgttc ttcgatgacg aagggaattt aacccatatc ggtgttgccg gttttgaaac
    10141 gttgctggaa accgtgcagg tgctggtcaa agactatgat ttcagtatca gcgatgccct
    10201 gcgcccgctc accagtagcg tagccggttt ccttaacctg accgggaaag gcgaaattct
    10261 gccaggcaat gatgctgact tgctggtcat gacgccagaa ctgcgcattg agcaggtata
    10321 cgctcgcggc aaactgatgg tcaaagacgg caaagcctgc gtgaaaggaa cgtttgaaac
    10381 ggcttaaacg gttgtcagcc gcccgaatgg acgtagaatg cccgcaaggg cggctgacag
    10441 agtaaaacgt aatggatgac tgtggtgcga ttttgcataa tattgaaacc aaatggcttt
    10501 atgattttct gaccctggaa aaatgccgca atttttccca ggcggcagtc agtcgcaacg
    10561 tctcgcaacc ggcattcagc cgccgcatcc gtgcgctgga acaggcgatt ggtgttgaat
    10621 tgtttaaccg ccaggtaacg ccgctgcaac tctcagaaca agggaaaatc ttccattcgc
    10681 agatccgcca tctgttgcaa cagttagaga gcaatctggc agagctgcgt ggcggcagcg
    10741 attacgcgca acgtaaaatc aagatagccg ctgcacactc tctttccctc gggctgttac
    10801 cgtccattat cagccagatg ccgccgctct ttacctgggc aattgaagct attgatgtcg
    10861 atgaagcggt cgataaactg cgtgaagggc aaagtgactg tattttttcc tttcacgacg
    10921 aagatttgct ggaagcgccg tttgaccaca ttcgcttatt tgaatctcaa ttgttccctg
    10981 tctgcgccag tgacgaacac ggagaagcac tttttaacct cgcgcagcca cactttccgt
    11041 tactgaatta cagccgcaac tcctacatgg ggcgattgat taatcgcacc ctgacgcgcc
    11101 acagtgagtt aagtttcagc accttttttg tctcttcgat gagcgagctt ttaaagcagg
    11161 ttgccctcga cggctgtggg attgcctggc tgccggaata cgctatccaa caagaaattc
    11221 gcagcgggca gctcgttgtg ctgaatcggg acgaactggt gatcccgatc caggatctac
    11281 gcataccgga tgaatactcg catgaatccc gttgccgaac gcttctggcg tgaactgcgc
    11341 gagctggaga ttgtgcttag ctgacattct ccagcatcgc actacaccaa ccgccattat
    11401 cccagaccag atcaaacgtt agcggttcac cgtcgtggta cagcattatc tcaaggctga
    11461 tcgccagagt gtggcagtac gcttctttaa aattaacctg caacgccgca atccgtggct
    11521 catgaaataa cagtgcagaa cgcagctgac ggcaatacca gctctggaga tgtttgtcgc
    11581 tctggttatg cacttgccac tcgggcatgc ctgaaacaag taatgacggt aatgacgaat
    11641 gaggagaatt aaaaagagtg aggagatcgc tgcataccga tttacgcagc aatgacgttt
    11701 tattgcctga aatttcaggt aaaacagcct gacgtgattg gcgcatcatt ttgcgcactc
    11761 ctgacgttta gcaacatatg cggcttgccc tgaacaccgg gcaccactgt attgatgctc
    11821 attttccata gccattccct tttgttcatt acatccatgt cagtctgaga caggcctgaa
    11881 tcctgtcagc aaagtataaa accttacttg cctatgaata tctactgact tttcaggaaa
    11941 aaagtcatga tttgataaaa atcatcaaac tcaaaaaaaa accgctagta tattctgaat
    12001 attccgaata gttacgtaga tgaaaatact tgtcaccacc gctacttaat tccggtgtga
    12061 aattatttat ttccgcataa tcagccatgt attatttgaa acaattagtt
//
LOCUS       DAZFWL020000137        12091 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFWL020000137 DAZFWL020000000
VERSION     DAZFWL020000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.137"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..262)
                     /locus_tag="ACHKNP_RS20080"
                     /old_locus_tag="pgaptmp_004016"
     CDS             complement(<1..262)
                     /locus_tag="ACHKNP_RS20080"
                     /old_locus_tag="pgaptmp_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706090.1"
                     /translation="MENVRFLQGVKQYFCTKALDVILLIYLFLGFILLPYKYLWKDII
                     LSLCFVGILLYALVEENRITEYMGYFVKFSNKNRLNSCAAWCA"
     gene            403..2835
                     /gene="hsdR"
                     /locus_tag="ACHKNP_RS20085"
                     /old_locus_tag="pgaptmp_004017"
     CDS             403..2835
                     /gene="hsdR"
                     /locus_tag="ACHKNP_RS20085"
                     /old_locus_tag="pgaptmp_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HGU4706091.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKAYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2902..4371
                     /locus_tag="ACHKNP_RS20090"
                     /old_locus_tag="pgaptmp_004018"
     CDS             2902..4371
                     /locus_tag="ACHKNP_RS20090"
                     /old_locus_tag="pgaptmp_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGU4706092.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     VLELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMKTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRIENEQAWKVSIDDVIARNFNLDIKNPHQAETI
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4371..6140
                     /locus_tag="ACHKNP_RS20095"
                     /old_locus_tag="pgaptmp_004019"
     CDS             4371..6140
                     /locus_tag="ACHKNP_RS20095"
                     /old_locus_tag="pgaptmp_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU4706093.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWTRLINLGIWALGSGFPNVVQGSTDKEILMCKVSDMNLEGNEKFIFSTKNTISK
                     DLADEYKIKISEPGTIIFPKIGGAIATNKRRILVQDTAIDNNCLGIKPCDAISGEWFY
                     LILNTLDMSKYQSGTSIPAINQSVIGSIPIALPSLKMQEKIVSYVITLMSLCDQLEQQ
                     SLTSLDAHQQLVETLLGTLTDSQNAEELAENWARISEHFDTLFTTEASVDALKQTILQ
                     LAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEG
                     WEWCKFGLTSEFINGDRGSNYPNKNEYVSQGIPWINTGHIEKNGTLTVTEMNFITEGK
                     FNELRSGKIQKGDLVYCLRGATFGKTAFVIPYETGAIASSLMIIRPFITEMGGYIYNY
                     LTSPFGRSQIYRFDNGSAQPNLSANSVMLYSFPCPPLTEQYRIFSQVGLLHELCDKLK
                     TRIKTAQQTQLHLADALTDAAIN"
     gene            6361..6702
                     /gene="symE"
                     /locus_tag="ACHKNP_RS20100"
                     /old_locus_tag="pgaptmp_004020"
     CDS             6361..6702
                     /gene="symE"
                     /locus_tag="ACHKNP_RS20100"
                     /old_locus_tag="pgaptmp_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HGU4706094.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPTAEESELMQSLRQVCKLSARKQKQVQDF
                     IGVITGKQKVA"
     gene            complement(6749..7912)
                     /locus_tag="ACHKNP_RS20105"
                     /old_locus_tag="pgaptmp_004021"
     CDS             complement(6749..7912)
                     /locus_tag="ACHKNP_RS20105"
                     /old_locus_tag="pgaptmp_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HGU4706095.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFMNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPSLSEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(7960..8842)
                     /locus_tag="ACHKNP_RS20110"
                     /old_locus_tag="pgaptmp_004022"
                     /pseudo
     CDS             complement(7960..8842)
                     /locus_tag="ACHKNP_RS20110"
                     /old_locus_tag="pgaptmp_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(8939..9103)
                     /locus_tag="ACHKNP_RS20115"
                     /old_locus_tag="pgaptmp_004023"
     CDS             complement(8939..9103)
                     /locus_tag="ACHKNP_RS20115"
                     /old_locus_tag="pgaptmp_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HGU4706096.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            9280..10692
                     /locus_tag="ACHKNP_RS20120"
                     /old_locus_tag="pgaptmp_004024"
     CDS             9280..10692
                     /locus_tag="ACHKNP_RS20120"
                     /old_locus_tag="pgaptmp_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU4706097.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSREHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWGAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQNYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(10934..11878)
                     /locus_tag="ACHKNP_RS20125"
                     /old_locus_tag="pgaptmp_004025"
     CDS             complement(10934..11878)
                     /locus_tag="ACHKNP_RS20125"
                     /old_locus_tag="pgaptmp_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGU4706098.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIYLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVNTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRALPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
BASE COUNT         3390 a         2947 c         2782 g         2972 t
ORIGIN      
        1 gtgcacacca ggcagcacaa ctattcaacc tgtttttatt actgaactta acaaaatacc
       61 ccatatattc tgtaatgcga ttttcttcca ctaatgcata aagaagaata ccaacaaagc
      121 acagagataa aataatatct ttccacagat atttataagg gagaagtata aaaccaagaa
      181 atagataaat tagcagaatg acatctaatg ccttagtaca aaaatattgt ttcactcctt
      241 gcaagaaccg aacattctcc atatgaatcc ttatcctact attgatgaaa ctcaagaata
      301 cctttaaaac agtgacttaa cgctgataat actctttgaa cagatgaatt catctatttt
      361 tctgtgtaga atttgcgcat cgcatttaga tgagaacacc ccatggcgga actgaaccta
      421 agtaacctga cggaagcaga catcattacc aaatgcgtta tgccagccat tctcaatgca
      481 ggctgggaca acacaacgca aatcagacag gaggtcaaac tccgggacgg taaagtcatc
      541 gtacgtggta aagttgcggc acgcagaacg gtaaaatctg ctgacatcgt gctgtatcac
      601 aaaccaggca ttcccttagc cgtgattgaa gcaaaagcca acaaacatga aattggcaaa
      661 gggatgcaac agggcattga atatgcgcgc ctgctggacg ttccttttgt ttttgccacc
      721 aacggggatg gctttatctt ccgcgatgcc accgcagccg aaggtgaatg ccttgaaaag
      781 caaataacgc tggatgactt cccctccccc gctgaactct ggcaaaaatt ctgcctctgg
      841 aaaggataca cacaagctca gcttccggtg attactcagg actattacga cgatggcagc
      901 ggtaaatcgc cacgttatta ccagcttcag gcaatcaaca aaaccattga agccgtctcc
      961 aacgggcaaa accgcgttct gctggtcatg gcgaccggaa cagggaaaac ctataccgca
     1021 ttccagatca tctggcgcct gtggaaatca aaaaataaaa aacgcatttt gttccttgcc
     1081 gatcgcaaca ttctggtcga ccaaaccaaa aataatgatt tccagccatt tggtacggca
     1141 atgaccaaag tcagcggacg caccattgat cccgcttatg aaattcacct cgcgctctat
     1201 caggctataa ctggcccgga ggaagaccaa aaagcgttta aacaagtcgc accagatttc
     1261 ttcgatctga tcgtgatcga cgaatgccat cgcggcagcg catccgaaga cagcgcctgg
     1321 cgagagatcc ttgattactt cagttccgcc acccaaattg gcttaaccgc cacgccaaaa
     1381 gagacgcatg aagtctccag cacggattac ttcggcgatc cggtttacgt ctactcgcta
     1441 aaagaaggga tcgaagacgg cttcctcgcc ccttataaag ttgtccgtgt tgatattgat
     1501 gttgatctgc aaggctggcg cccaaccaaa gggcaaactg acttaaacgg cgaagtgatc
     1561 gacgatcgta tctataacca gaaagatttc gatcgcacga tggtaatcga cgaacggaca
     1621 gaactggttg ccagaaccat taccgactac ctcaagcgca ccaacccgat ggataaaacc
     1681 atcgtcttct gtaacgacat cgatcatgca gaacgtatgc gccgcgccct ggtgaatctc
     1741 aacccggagc aggtgaaaaa gaacgacaaa tacgtcatga aaatcaccgg cgatgatgaa
     1801 attggcaaag ctcagttgga taacttcatc aacccgaaaa aagcgtaccc ggttatcgcg
     1861 accacttcag agctgatgac cactggtgtg gatgctaaaa cctgcaaact ggtagtactg
     1921 gaccagaaca tccagtcgat gaccaaattc aagcaaatta tcggtcgtgg tacacgcatc
     1981 gacgaacgtt acggcaaact ctggtttacc atcctcgact ttaaaaaagc caccgaactg
     2041 tttgccgatg agcgtttcga tggcattccc gaaaaagtca tggataccac accagaggat
     2101 atcgccgatc cagaatctga ttttgaagag aaactcgaag aaatcagcga acatgacgac
     2161 gaacaggtaa caggcgttga tgaaccgcct gcgccaccat accaggttac cgataccgat
     2221 gatgtcggcc cacttccgga agaagacgag aagaaaatcc gcaagtttca cgtcaacggt
     2281 gtagcggtgg gcgttattgc gcagcgtgtt cagtattacg atgccgacgg taaactggtt
     2341 accgaatctt ttaaagatta cacccgcaaa acactgctca aagaatatgc ctcgctggat
     2401 gactttaccc gcaagtggca ggacgccgat cgcaaagaag cgatcattca cgagctggag
     2461 caacagggga tcatctggga agtactggca gaagaagtcg gtaaagatct cgacccgttc
     2521 gacatgcttt gccacgtagt gtatggtcag ccgccgttaa cccgcaaaga gcgcgccgag
     2581 aacgtgcgca agcggaacta cttcacaaaa tactctgaag cagcgcaagc cgtgctcgat
     2641 aatctgctgg ataaatacgc cgatgcgggc gtacaggaga tcgaaagtat tcaggtgctg
     2701 aaacttaaac cattcgacag catgggcact ttaccggaga ttattaaaac cggatttggc
     2761 gaccgtaacg ggtataatca ggcgctcagc gagctggaaa acgaaatcta ccaattaccg
     2821 ccccgctctg cttaacaact ctcttttgtg ccttctgcaa ccaacatgtt gcagaaggcc
     2881 ttgtttttca tggaatacaa catgtctatc agttcagtaa tcaaatcatt acaagatatt
     2941 atgcgcaaag atgccggtgt ggatggcgat gcgcagcgtc tcggtcagct ctcctggctg
     3001 ctgtttttga aaatcttcga tgcccaggaa gaggtgctgg aactggagca ggataactat
     3061 caatatccga tcccacagcg ttatttatgg cgcagttggg ccgcaaacgc tcagggcatt
     3121 accggtgatt ccctgctgga attcgttaat gatgatctgt tcccggcgtt gaaaaacctc
     3181 actgcgccta tcgataaaaa cccacgcggc tacgtggtga agcaggcgtt cagcgatgcc
     3241 tataactata tgaaaaacgg tacgctactg cgccaggtga tcaacaagct gaacgaaatt
     3301 gactttacca gcgccagcga acgccatctg tttggtgata tttacgaaca gatccttaaa
     3361 gatctgcaat ctgcgggcaa tgctggcgaa ttctatacgc cacgcgccgt cactcgcttt
     3421 atggtggatc gcgttgatcc gaaactcggc gaatccatta tggacccggc ctgcggtacg
     3481 ggcggttttc ttgcctgcgc atttgatcat gtaaagaaca aatacgtgaa gagcgtcgcc
     3541 gatcatcaga cgctgcaaca acagatccac ggtgttgaga aaaaacagct tccgcacctg
     3601 ctggcgacca ccaatatgct gctgcacggc attgaagtac cggtacaaat tcgtcacgac
     3661 aacaccctga acaaaccgct ttcctcctgg gatgaacaac tggatgtcat tgttaccaac
     3721 ccgccgtttg gtggcacgga agaagacggt attgagaaga actttccggc agagatgaaa
     3781 acccgcgaaa cggcggatct gttcctgcaa ctgattgtgg aagtactggc gaaaaacggt
     3841 cgcgcggcgg tggtattgcc ggatggcaca ctatttggcg aaggcgttaa aaccaaaatc
     3901 aaaaaactgc ttaccgaaga gtgcaacctg cataccatcg tgcgtttacc gaatggggtg
     3961 tttaacccct ataccggcat taaaaccaac ctgttgttct ttaccaaagg tcagccaacc
     4021 aaagagattt ggttctatga gcatccgtat ccggcgggcg taaaaaacta cagcaaaacc
     4081 aagccgatga agtttgaaga gtttcaggcg gagatcgact ggtggggtaa cgaggccgat
     4141 ggttttgcca gccgcatcga gaatgagcaa gcgtggaaag tcagcattga tgacgtgatt
     4201 gcgcgtaact tcaatctgga tattaaaaac ccacatcagg cggaaaccat tagccatgat
     4261 ccggacgaac tgttagcgca gtatgcaaaa cagcaggcgg agatccagac gctgcgtaat
     4321 caactgcgcg atattcttgg cgctgcgctg tctggcaagg aggttaacta atgagtgtgg
     4381 aaaagctgat cgttgatcat atggaaacct ggacctcggc gttgcaaacc cgttccaccg
     4441 ccgggcgcgg cagctccggt aagattgatt tgtatggtat taagaaattg cgtgagctga
     4501 ttctggaact ggcggtgcga ggtaaactgg tgccgcagga tccgaacgat gaaccggcgt
     4561 cggagctgct gaagcgtatt gcggcggaaa aagcagagct ggtgaagcaa ggtaaaatta
     4621 aaaagcaaaa accgctgccg gaaattagcg aggaagagaa gccgtttgaa ttgccggagg
     4681 ggtgggaatg gactcgatta attaatcttg gtatatgggc tttagggtct ggttttccaa
     4741 atgtggtaca gggaagcact gataaagaaa ttttgatgtg caaagttagc gacatgaatc
     4801 ttgaaggtaa cgagaaattc attttttcga caaaaaatac aatttcaaaa gacctagctg
     4861 atgaatataa aattaaaatc tcagaacccg gcaccatcat tttccctaaa attggcggag
     4921 caattgcaac taataaacga agaattttag ttcaggatac agctattgat aacaactgtc
     4981 ttggaataaa accatgtgat gcaatatcgg gggagtggtt ctatctaatc cttaatactc
     5041 tcgatatgtc gaagtatcaa tcaggaacct caatacccgc gataaatcaa agtgtcatag
     5101 gtagtatacc aattgcgctt ccttctttaa aaatgcaaga gaagattgtt tcttatgtca
     5161 taaccctaat gtccctttgc gaccaactgg aacagcaatc cctgaccagt ctggacgcac
     5221 atcagcaact ggttgaaacc ctgttgggaa cacttacaga cagccaaaac gccgaggaac
     5281 tggctgaaaa ctgggcgcgt attagcgagc atttcgacac cctatttacc accgaagcca
     5341 gcgtggatgc gttaaaacag accattctgc aactggccgt aatgggcaaa cttgtgccgc
     5401 aggatccgaa tgacgaacca gcctctgaac tgctcaaacg aattgcgcag gaaaaagctc
     5461 aactggtgaa agaaggaaaa ataaaaaaac aaaaaccgtt gccgccaatt agcgatgagg
     5521 aaaaaccgtt tgaacttccg gaagggtggg agtggtgtaa atttggttta acatcagagt
     5581 ttataaatgg cgatcgtgga agtaattacc ccaataaaaa tgagtatgtt tctcaaggta
     5641 ttccttggat caatactgga catatagaaa aaaatggtac acttacggtt acagaaatga
     5701 attttataac cgaaggaaaa tttaatgaat taagaagtgg gaaaattcaa aaaggtgatt
     5761 tggtttattg cctacgagga gcaacttttg gtaaaactgc ctttgtaatc ccctatgaaa
     5821 caggagctat tgcctcatca ctaatgatca ttagaccatt tatcacagaa atgggagggt
     5881 atatttataa ttatttaacc agtccatttg ggcgctcaca gatttaccgc tttgataatg
     5941 gctcagcaca acccaaccta tcagcaaata gtgtaatgtt gtattcattc ccatgcccac
     6001 cattaactga acaatataga atattttcac aagtaggttt acttcatgaa ctatgcgata
     6061 agcttaaaac tcgtataaaa acagcccaac aaacccaact gcaccttgca gacgcactca
     6121 ctgacgcggc gataaactaa ttttctcgcc ctcaaaaaac caataaagcg gctaaatggc
     6181 cgctttattc acatcaccaa aaattatatt tcacgctaat tttcatctta ttttcattaa
     6241 tttttagctg gactttccct gtatttactg atgatatatc caggtattta gcgcggtgcg
     6301 gatgtgcgcc aacacacccg caccgctaat cacaatcact attatcggag aatagcagtt
     6361 atgactgaca cgcattctat tgcacaaccg ttcgaagcag aagtctcccc ggcaaataac
     6421 cgtcaattaa ccgtgagtta tgcgagtcgc tacccggatt acagccgtat tcccgccatc
     6481 accctgaaag gtcagtggct ggaagccgcc ggttttgcca ccggcacggc ggtagatgtc
     6541 aaagtaatgg aaggctgtat tgtcctcact gcccaaccac ccaccgccga agagagcgaa
     6601 ctgatgcagt cgctgcgcca ggtgtgcaag ctgtcggcgc gtaaacaaaa gcaggtgcag
     6661 gattttattg gggtaattac cggtaaacag aaagtcgcct gataaattga cccggtagcg
     6721 ccagtgctac cgggtattat tcgccgcgtt ataccaacgc gctctccatc tgtttttcaa
     6781 atgcctgacg aattaatccc ataatgtaag gaagttgtgc gaggtcgctg aaaccaattt
     6841 ctacatcgcc attaccccag cgcccaacat ttgtcacatc tttggcaata cctttcggat
     6901 cgaccagttc atgaaactgc atattaagcg acaaccgcag tcggcttttt tgcggtacaa
     6961 catcaacaaa attcgtttca gctttaaacg caatatagag cttcaatact tcctgcgtga
     7021 tccctgcgtc caggcgcata acttcatcgc gaagatgatc gaataacacc cggctatgcg
     7081 aaccatcagc aagaaaagga taatcactca ggctgtaact ggttttgttt tcaggctgcc
     7141 cccgataaac agctaaaacc tcttcagaaa gagaaggcgc ttgccagacc tgaaccgcga
     7201 gatcggccag acgatcggca cgggcgcgaa tggcggcttc atcccatttt tcgcactgcc
     7261 caagaccgat attcaaatag agcgggctat gtttaaagcc gtcttcaata tcgcgttttt
     7321 ccgcgaaagg tctgtcactg tagcgggagt tatagcgcgt taaagtgaga ttccccaacg
     7381 tatgtaacag ttctttatgc acacgctgcc agtcgcttcc cagctcttca cgccatttag
     7441 ccgacagatt ttcgttctgc ggcataatgt gctcaatcgt aaactcttcc acgcgctctc
     7501 tgcgtttatc gttttccagt cttcgtaacc agtagctgcg gctgcggaag ttatagagat
     7561 cgcgaacttt taattcccgt ttaaattcat catcgttggg gaaacgacga tatgaaggca
     7621 ggttcataaa atgtacctga atactttcca gatatttttc tttattaatg accttataaa
     7681 aagtggcaaa cgtcttgttc aaagaattcg tcggaattgc acatactgca cggcggaaaa
     7741 cataagattc aattaaacga attatgctca ggaaatcttc gtgagacaaa tcgtcatttt
     7801 tatagtcatg ataaagcgcc agtaagaaag gatacgccac atcaaccttt aactcgcgca
     7861 aatcctgaaa agccgtagca agcgatttgt cactttcttt tcccaatgcc attgcacagt
     7921 aatactccgc gtagatatga atatcttcaa ccagccgatc tacgcctttt tcagcaacac
     7981 tctggctgcg ggcatggagt ttaaatgcct catagacttc atctgtccga gggatctccc
     8041 ccgtttttac cgtcagataa tgacgcataa aactgtcaaa atattcgctg taaccttgct
     8101 gaccaaaggc gacttccatt ggacgccagt gatcttcata caaccgggtt tgatgctctg
     8161 gttcgaggcc catcagaata aaattgcgca ccagatcggc ctgacttaac gccttaccgg
     8221 tggagttcat actttcaaaa atcagttgcg gattatcctg accacgatta agcgccacat
     8281 cgacaattaa taactttgct aacccacgac ataaggggat caagtcatca ccgagtttgg
     8341 caatttgttc atcaaagaaa gtaaagtttt ccattattcg atgggaatag ttttctggca
     8401 ttggtctttg ttttattaac gccagtaaac tgtctttatc agtctcggtc agtagtaatt
     8461 taaaaccttt ctcgccggac tcataaggat tgagcaaata ataattacgc aatttcattg
     8521 ctgaaaagcc atcaaaaact tcgtcttcgc caagatggcg cgataaagcc tcaatcagca
     8581 acattgcggt cgtcagccgt tgttgaccat caatgaccag taacggagaa ataccagaaa
     8641 cctgatacaa cccctgctca atataaacaa ccgaaccgat aaaatgcgct gatatatcgt
     8701 cacgctttcc ggcacgaatg atgtcgtccc aaagttgccg acattgttgt tctgtccatg
     8761 aataagtccg ttgataaatg ggaataacaa actgctgcga acgttttaaa aaatcgagta
     8821 ggcgggcttc tgtagctttc attctgacgt ctttccgtga agcgatgatt tcgcaataat
     8881 aaccacttcc ttttggaggt tatagagaaa tcctgtttat ccgcgatgaa ttgccccctc
     8941 actccacatc ctccctgcgt aacccaatat ctctcaaccg ctcatcactc atctgctgta
     9001 acactcgtcg ggtctgcatt tgccgccgcc aacgcctcac cgcctgccag agttgcacaa
     9061 gtctgataaa cggcgttttg gctttgtttt cgtgaaattc catctgctgc ctcctcatct
     9121 ttcagaggct tcatcatcca ccttctggca gttcacaata cagatgcaaa tattagtttt
     9181 atttaacata cagacaggtt aaaacagcat ctgcatccgg ttttttagcc tcatctgtac
     9241 cggtttttct ttctgtatgg tccaaataag gaatacagca tgacgcgtta tcaacatctg
     9301 gcgactctac ttgccgagcg gattgagcaa gggctgtatc gtcatgggga gaaattgccg
     9361 tcggtgcgta gcttaagtcg ggagcacggc gtcagcatca gcaccgtgca gcaggcgtat
     9421 cagacgctgg agacgatgaa gctcataacc ccacagccgc gttcgggtta ttttgtcgca
     9481 caacgtaaag cccagccgcc agtgccgccg atgacgcgtc cggtgcagcg cccggtggaa
     9541 attacccagt gggatcaggt gctggatatg ctggtggcgc atagcgacag ttccattgtt
     9601 ccgttaagca aaagcacgcc ggatgtcgaa acgcccagcc tgaaaccgct ctggcgggag
     9661 ctaagccggg tggtgcagca taatctgcaa accgtgctcg gttatgactt gttagccggt
     9721 cagcgggtat tgagagagca gattgcccgc ctgatgctcg acagcggctc ggtggtcacc
     9781 gccgatgaca tcattatcac cagcggctgc cataactcga tgtcgctggc gttaatggcg
     9841 gtgtgtaaac cgggcgatat tgtcgcggtc gaatctccct gttattacgg ttcgatgcag
     9901 atgctgcgcg gcatgggcgt gaaagtgatt gaaatcccaa ccgatccaga aacaggcatc
     9961 agcgttgaag cgctggaact ggcgctggaa cagtggccga ttaaaggcat cattctggtg
    10021 ccaaactgta ataatccgct gggatttatt atgccggacg cacgcaaacg ggccgttctc
    10081 tctctcgctc agcgtcatga tattgtgatt tttgaagatg atgtctacgg cgaactggca
    10141 acggagtatc cgcgcccgcg gaccatccat tcatgggata tcgacgggcg agtgctgttg
    10201 tgcagctcgt tcagtaaaag tattgctcca ggcctgcgcg tgggttgggg cgcaccgggg
    10261 cgttatcacg ataaactgat gcatatgaaa tacgccatca gcagctttaa tgtgccgtcc
    10321 acgcaaatgg cggcggcaac gtttgtgctg gaaggtcact atcatcgcca tatccggcgg
    10381 atgcggcaga actatcagcg caatttggcg ctttatacct gctggatacg ggaatatttt
    10441 ccctgcgaaa tctgtattac gcgcccgaaa ggcggatttt tactgtggat agaattgcct
    10501 gaacaggtcg atatggtctg cgttgcgcgg cagctgtgcc gcatgaatat ccaggtggca
    10561 gcaggctcga ttttctcggc ttccggcaaa taccgtaatt gtctacgcat caactgcgct
    10621 ttgccgctca gcgaaaccta tcgcgaagca ctaaagcaaa ttggcgaggc cgtgtatcgg
    10681 gcaatggaat aaattcgaat tagtggcgaa gcacctgatt tcctctcacg aatcgcctgt
    10741 aactcgttgt atctttgcag ggtcatcgcc acgttccagc atggcttagg caaagtgtgc
    10801 ttcgttcata ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccgc gcaaattcaa
    10861 tagattgcag aggaaatgta ggtctgataa gcgtagcgca tcaggcagct ttgcgtttgt
    10921 catccgtctc tgattagtat tcgatccatg gatcgcgctt gatttcaggc aagggctgct
    10981 ctaatgcctg catttgctct gtcgaaagtc cggtaatctt ttgtatccat tcaggatctg
    11041 ccccattctg caacaacaat ctaaggaggc gttgttcccc ttcttctttc cccttttcca
    11101 ttcccaacag ccatccatca ttataaattc gctctgcaat cgtcattatt cgctccctgt
    11161 gttgtggcat tcgacgggta agttcgctga taaacttctt aaaacgcgct tcatcgccag
    11221 tcagtaaaat gtaatttaac agcgcagtta tctggctgtc attagcacac tctgtaacca
    11281 gtaatactac cagttggtcg ataagcccca tcagatcgcg ctggcgaata tgcttttgga
    11341 tcaactccaa cagggcgact ctgcgatgct gcacaatctc gtcgtctggc acgacagtga
    11401 tatccaccag cgggaacgcg gcggtataaa gtttccgtgc ggtagttggg tcggcaaatt
    11461 cgtccagcca gcacagggac cagggataag gactacggct accgtgataa aatagcatcg
    11521 gtataaccag cggtagcgcc cgacgtttat catgctcgat atggcgctgc atcaccgcca
    11581 tggaatagcg catcaggcga aaggccatat gaatatcctc acggctctga tgttcaatca
    11641 ccacataaat atagccatcg ccttcgcggg tatttaccga ccacagaata tcggagtgta
    11701 gcgcccgcaa tttttcatcg acaaagctgg cggattccag ttttaagctg tcgagatcgc
    11761 acagttcacg taaatctttg ggtaaataga tctccataaa atcccgcgcg gtgtcagggt
    11821 gggtgagaaa ggatttaaat aatgcgtcat gcggcgtgct ggttgtgaag tttgtcatgg
    11881 cgatccgtca cctgttgata agatggcccg actttagcga ctaacaaaac cattcacacc
    11941 tggcaatttc ccgttccccg aggcgctttc caggctattt ttgttcctct gcaagcgata
    12001 catttttccg gaaacaggct cgcgttttac atttcctgta cccggtagat attccccttt
    12061 ccaccgcgct cttttgatta atcgcaaatt t
//
LOCUS       DAZFWL020000138        11840 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFWL020000138 DAZFWL020000000
VERSION     DAZFWL020000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.138"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..399
                     /locus_tag="ACHKNP_RS20130"
                     /old_locus_tag="pgaptmp_004026"
     CDS             <1..399
                     /locus_tag="ACHKNP_RS20130"
                     /old_locus_tag="pgaptmp_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGU4706099.1"
                     /translation="IIKQSKNLNFLNVSGKNNCPTMQLMLFNLFSPALKLNTGLAILS
                     PGAFEVHSDGIDVDNELFHYPIKKAYTPYNIHTYKTEEVVNQRNIKVKNMTLDEINNT
                     YCNNDYYNQAIREEPIDLLDRSFSSSSWPF"
     gene            723..2111
                     /gene="narU"
                     /locus_tag="ACHKNP_RS20135"
                     /old_locus_tag="pgaptmp_004027"
     CDS             723..2111
                     /gene="narU"
                     /locus_tag="ACHKNP_RS20135"
                     /old_locus_tag="pgaptmp_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HGU4706100.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2193..5933
                     /gene="narZ"
                     /locus_tag="ACHKNP_RS20140"
                     /old_locus_tag="pgaptmp_004028"
     CDS             2193..5933
                     /gene="narZ"
                     /locus_tag="ACHKNP_RS20140"
                     /old_locus_tag="pgaptmp_004028"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HGU4706101.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5930..7474
                     /gene="narH"
                     /locus_tag="ACHKNP_RS20145"
                     /old_locus_tag="pgaptmp_004029"
     CDS             5930..7474
                     /gene="narH"
                     /locus_tag="ACHKNP_RS20145"
                     /old_locus_tag="pgaptmp_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU4706102.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7474..8167
                     /gene="narW"
                     /locus_tag="ACHKNP_RS20150"
                     /old_locus_tag="pgaptmp_004030"
                     /pseudo
     CDS             7474..8167
                     /gene="narW"
                     /locus_tag="ACHKNP_RS20150"
                     /old_locus_tag="pgaptmp_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
     gene            8164..8844
                     /gene="narI"
                     /locus_tag="ACHKNP_RS20155"
                     /old_locus_tag="pgaptmp_004031"
     CDS             8164..8844
                     /gene="narI"
                     /locus_tag="ACHKNP_RS20155"
                     /old_locus_tag="pgaptmp_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU4706103.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKREMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8923..9816
                     /locus_tag="ACHKNP_RS20160"
                     /old_locus_tag="pgaptmp_004032"
     CDS             8923..9816
                     /locus_tag="ACHKNP_RS20160"
                     /old_locus_tag="pgaptmp_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HGU4706104.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9912..10757)
                     /gene="nhoA"
                     /locus_tag="ACHKNP_RS20165"
                     /old_locus_tag="pgaptmp_004033"
     CDS             complement(9912..10757)
                     /gene="nhoA"
                     /locus_tag="ACHKNP_RS20165"
                     /old_locus_tag="pgaptmp_004033"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HGU4706105.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10930..11499
                     /locus_tag="ACHKNP_RS20170"
                     /old_locus_tag="pgaptmp_004034"
     CDS             10930..11499
                     /locus_tag="ACHKNP_RS20170"
                     /old_locus_tag="pgaptmp_004034"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HGU4706106.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11503..11730)
                     /gene="pptA"
                     /locus_tag="ACHKNP_RS20175"
                     /old_locus_tag="pgaptmp_004035"
                     /pseudo
     CDS             complement(11503..11730)
                     /gene="pptA"
                     /locus_tag="ACHKNP_RS20175"
                     /old_locus_tag="pgaptmp_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
BASE COUNT         2842 a         3041 c         3085 g         2872 t
ORIGIN      
        1 ataataaaac agagcaaaaa cttaaatttt ttaaatgttt ctgggaagaa caattgccct
       61 actatgcagc tcatgttatt taatttgttt tccccagcac ttaagcttaa tactggcctg
      121 gcaattcttt cgcctggtgc atttgaagtt cactctgacg gaatagatgt ggataacgaa
      181 ttgtttcact atcctattaa aaaagcatat accccatata atatacacac ttacaagaca
      241 gaggaagttg taaaccagag gaatataaaa gttaaaaaca tgaccttaga tgaaataaac
      301 aatacttact gtaataacga ttattacaat caggcaataa gagaggaacc gatagacctt
      361 ctggacagat cgttttcctc cagttcatgg cctttttagt gaataaatca tcactaattc
      421 tataagtgtc tggtcttgtt gataatatag ttaatatgta accctttaga ttcacgatct
      481 gaagggttga aacatgacat acgttcaaag attaaccccc gtttgaaggt tagcttgtta
      541 gttacattta gtaacacata tctggctact taatccccat tctgcccccg cggttacgct
      601 ttcttatcta ttctttcaat cagcacttca cccaattcat ccttaattct aataactggc
      661 tattgcgcct tacctcctgt tttgtctttt ttcattacca atgtgtgcat gtgaggaaca
      721 atatggcact gcaaaatgag aaaaatagtc gttatctttt acgcgactgg aaaccagaaa
      781 atccggcctt ctgggaaaat aaaggaaaac atattgctcg aagaaacctc tggatatcag
      841 tcagttgtct acttcttgcc ttctgtgtct ggatgctatt tagcgcagtt accgttaatc
      901 tcaataaaat cggttttaat tttactaccg atcaactctt tttattaacc gcattaccct
      961 ccgtttctgg cgcattattg cgtgttccct actcctttat ggtgcctata ttcggtggac
     1021 gccgatggac ggtttttagt actgcaatcc tgattattcc ttgcgtctgg ctcggaattg
     1081 ccgtgcaaaa tccgaatact ccttttggga tatttatcgt tatcgctttg ctatgcggtt
     1141 ttgcaggtgc aaactttgct tcgagcatgg gcaatatcag tttcttcttt ccaaaagcca
     1201 aacaagggag cgctcttggg attaatggcg gattaggaaa cttaggtgta agtgtaatgc
     1261 agctggttgc accgctggtc atttttgtac ctgtatttgc ctttctcggc gtcaatggcg
     1321 taccgcaggc cgacggttcg gtgatgtcgc tggcgaatgc cgcatggatt tgggtaccgc
     1381 tactggcgat tgccacgatc gccgcatggt cagggatgaa tgatatcgcc agttcacgcg
     1441 cctcaattgc cgaccagctc cctgtcttac aacgcctgca tctctggctg ctgagcctgc
     1501 tttaccttgc caccttcggt tcgtttatcg gtttttctgc gggttttgcc atgctggcaa
     1561 aaacccagtt cccggatgtg aatattctgc gcctggcgtt ctttggccca tttatcggtg
     1621 ccatcgcgcg gtcggttggt ggtgctattt ccgataagtt cggcggcgtg cgggtgacgt
     1681 tgatcaactt tatttttatg gcgattttca gtgccctgct gttccttacc ttaccgggca
     1741 caggctccgg taatttcatc gccttttacg ccgtatttat ggggctgttt ctgactgcgg
     1801 gtctgggaag tggttctact ttccagatga tcgccgtcat ctttcgccag ataaccattt
     1861 atcgggtaaa gatgaaaggc ggtagtgatg agcaagctca gaaagaagcc gtcaccgaaa
     1921 cggcggcggc tctgggcttt atctcagcca ttggcgcagt gggcggcttt tttattccgc
     1981 aggcgtttgg catgtcgctc aatatgaccg gctctccggt cggcgcgatg aaagtgtttt
     2041 taatcttcta catcgtttgt gtgctgctga cctggctggt ttatggtcgg cggaagttca
     2101 gccaaaaata agagatcgat gcctgatgta caaccgtgtc gcatccgaca aggcatcaga
     2161 tcccaattca ttcctggagc aggagtcatg tcatgagtaa acttttggat cgctttcgct
     2221 acttcaaaca aaagggcgaa acctttgccg atggtcacgg acaggtgatg catagcaacc
     2281 gcgactggga ggacagctat cgccagcgtt ggcagttcga caaaatcgtg cgttccaccc
     2341 acggtgttaa ctgtacaggc tcctgtagct ggaaaatcta cgttaaaaat ggtctggtga
     2401 cctgggaaat ccaacagacc gactacccgc gcactcgccc tgacctgccc aatcatgaac
     2461 ctcgcggctg cccgcgtggc gcaagttact cctggtatct ttacagcgct aaccgcctga
     2521 aatacccgct cattcgtaaa cgactgattg aactgtggcg cgaagccctc aagcaacaca
     2581 gcgatccggt actggcgtgg gcatcgatta tgaacgatcc gcaaaagtgc ctgagctaca
     2641 aacaagtgcg tgggcgcggc gggtttatcc gctccaactg gcaggaacta aaccagctga
     2701 ttgccgccgc taacgtctgg accatcaaaa cctacggccc ggatcgcgtt gccggtttct
     2761 cgccgatccc ggcgatgtcg atggtttctt acgccgccgg aacgcgttat ctgtcgctgc
     2821 ttggcggcac ctgtttaagt ttctacgact ggtattgcga cctgccgccc gcctcgccga
     2881 tgacctgggg cgagcaaacc gacgtaccgg aatctgccga ctggtataac tccagctaca
     2941 tcatcgcctg ggggtctaac gtaccgcaga cacgtacgcc ggacgcccac ttctttaccg
     3001 aagtacgcta caaaggcact aaaaccatcg ccattacccc tgactactct gaagtggcca
     3061 aattgtgcga ccagtggctg gcaccgaaac aaggcactga tagcgccctg gcgatggcaa
     3121 tgggccatgt gattttaaaa gagtttcatc tcgataatcc cagcgactac tttatcaact
     3181 actgccgccg ctacagcgac atgccgatgc tggtaatgct ggagcctcgc gacgatggta
     3241 gctacgttcc cgggcgcatg atccgcgcat ctgacctggt ggatggactg ggcgaaagca
     3301 acaatccgca gtggaaaacc gtagcagtta ataccgcagg tgaattggta gtgccgaacg
     3361 gttcgattgg tttccgctgg ggagaaaaag gcaaatggaa tctggaatcc attgccgccg
     3421 gtacggaaac cgaattgtcg ttaaccctgc tcggtcaaca tgacgctgtt gcaggcgtgg
     3481 ccttccccta ctttggcggc attgaaaatc cgcattttcg cagcgtaaaa cacaatccgg
     3541 tgctggtgcg ccaattgccc gttaaaaacc tgacgttagt cgatggcaac acctgtccgg
     3601 tggtcagcgt ttatgatttg gtactggcga attacggcct cgatcgcggg ctggaagatg
     3661 aaaacagtgc gaaagattac gctgaaatca aaccgtacac cccagcctgg ggtgagcaaa
     3721 ttaccggcgt gccgcgccag tatattgaaa ccatcgcccg tgaatttgcc gataccgccc
     3781 ataaaacgca tgggcgctcg atgattatcc tcggcgcagg tgttaaccac tggtatcaca
     3841 tggacatgaa ctaccgcggg atgatcaata tgctgatctt ctgcggttgt gtcgggcaaa
     3901 gcggtggcgg ctgggcacac tatgtcggcc aggaaaaact gcgcccacaa accggctggt
     3961 tgccgctggc ctttgcgctc gactggaacc gaccaccacg ccagatgaac agtacctcgt
     4021 ttttctacaa tcattccagc caatggcgct atgaaaaagt ctctgctcag gagttacttt
     4081 caccgctcgc cgatgccagt aagtacagcg gtcatctgat tgatttcaac gttcgcgccg
     4141 aacgtatggg ctggctacct tctgcgccgc agctggggcg taacccgctc gggattaaag
     4201 ctgaagccga caaggccgga ttatccccca cagaatttac cgcccaggcg ctgaaatcgg
     4261 gcgatttacg tatggcctgc gaacaaccag atagcggcag caatcatccg cgtaatttgt
     4321 ttgtctggcg ctctaacctg cttggctcct ccggcaaagg ccacgagtat atgcagaagt
     4381 atctgctggg gaccgaaagc gggattcagg gcgaggaact cggtgccagc gacgggatca
     4441 aaccggaaga agtcgagtgg caaactgcgg cgattgaagg caagctcgac ctgctggtga
     4501 cgctcgactt ccgcatgtcc agtacctgcc tgttctccga tatcgttctg cccaccgcca
     4561 cctggtacga aaaagacgat atgaacacct cggatatgca tccgtttatt catccgcttt
     4621 ctgcggcggt cgatccggcg tgggaatcac gcagcgactg ggaaatctac aaaggtatcg
     4681 ccaaagcatt ttcgcaagtg tgcgtgggtc atcttggcaa agaaaccgac gtggtattac
     4741 aacccctact gcatgactct ccggcagagc tctcacagcc gtgtgaagtg ctcgactggc
     4801 gcaaaggcga atgcgatctg attccaggca aaaccgcgcc gaatattgtg gcggtggagc
     4861 gcgactaccc tgctacgtat gaacgcttta cctcgctcgg gccattgatg gacaaacttg
     4921 gcaatggcgg taaagggatt tcgtggaata cgcaggatga aatcgatttc ctcggcaaac
     4981 tcaattacac caagcgtgat ggcccggcac aggggcgtcc gctgattgac accgccattg
     5041 acgcttcaga agtgattctg gcactggcac cggaaaccaa cggtcatgtt gcagtcaaag
     5101 cgtggcaggc gctgggcgag atcaccgggc gcgaacatac ccatctggcg ctgcacaaag
     5161 aggacgagaa gattcgcttt cgcgatattc aggcgcagcc gcgtaaaatt atctccagcc
     5221 ccacctggtc cggtctggaa agcgatcacg tatcctataa cgcgggatac accaacgttc
     5281 atgagttaat tccgtggcgc acgctgtcgg gacgccagca gctctatcag gatcatccgt
     5341 ggatgcgtgc ttttggtgaa agcctggtgg cttatcgccc gcctatcgac acccgtagcg
     5401 tcagtgagat gcgccagata ccgccaaacg gcttcccgga aaaagcactt aacttcctga
     5461 cgccgcacca gaaatggggc attcactcaa cctacagtga aaacctgcta atgctgacgc
     5521 tctctcgcgg tggaccgatt gtctggatca gcgaaacaga tgcccgagaa ctgaccattg
     5581 tcgataacga ctgggtggaa gtattcaacg ccaacggcgc gctgactgcc cgcgcggtgg
     5641 tcagccaacg tgtaccgccg ggcatgacca tgatgtatca cgcccaggaa cgcattatga
     5701 atattcctgg ttcggaagta actggcatgc gcggcggcat tcataactcg gtcacccgcg
     5761 tttgcccgaa accaacgcat atgattggcg gttacgcgca gctggcctgg ggctttaact
     5821 actacggcac cgtcggatcg aaccgcgatg agttcatcat gatccgcaag atgaagaacg
     5881 ttaactggct ggatgatgaa ggtcgcgatc aggtacagga ggcgaaaaaa tgaaaatccg
     5941 ttcacaagtc ggcatggtgc ttaacctcga caaatgtatc ggctgccata cctgttcggt
     6001 gacctgtaaa aacgtctgga ccgggcgcga aggcatggag tacgcatggt ttaacaacgt
     6061 cgaaaccaaa ccgggcattg gttatccgaa aaactgggaa gatcaggaag agtggcaagg
     6121 cggctgggtc cgcgatgtga atggcaagat ccgtccgcgt ctgggcagca agatgggcgt
     6181 aataaccaaa atcttcgcca acccggtggt gccgcagatt gatgattact acgaaccttt
     6241 caccttcgac tacgaacatt tgcatagcgc accggaaggc aaacatatcc ctactgctcg
     6301 cccgcgttca ctgattgacg gcaaacggat ggacaaagtg atctggggcc caaactggga
     6361 agaactgctg ggcggcgagt ttgaaaaacg tgcccgcgac cgcaacttcg aggccatgca
     6421 aaaggagatg tacgggcagt ttgaaaacac cttcatgatg tacctgccgc gcctgtgcga
     6481 acactgcctc aatcccagtt gcgtggcgac ctgcccaagc ggcgctatct acaaacgcga
     6541 agaagacggc attgtgctga ttgatcagga taaatgccgt ggctggcgtt tgtgcattag
     6601 cggttgtccg tacaaaaaaa tctacttcaa ctggaaaagc ggcaagtcag aaaaatgtat
     6661 cttctgttat ccgcgaattg agtcaggcca acccaccgtg tgctcagaaa cctgcgtggg
     6721 tcgcatccgg tatctgggcg tgctgcttta cgacgccgac cgcattgagg aagcggcgag
     6781 caccgagcgc gaagttgacc tctatgaacg tcagtgcgaa gtattcctcg atccacacga
     6841 tccctcagtg atcgaggaag ccctgaaaca aggtattcca caaaacgtga ttgaagctgc
     6901 ccagcgttcg cctgtctaca aaatggcgat ggactggaaa ctggcgctac cgctgcaccc
     6961 tgaatatcgc accctgccaa tggtctggta cgttcctccg ctgtcgccga ttcagtccta
     7021 cgcagatgcg ggcggtttgc cgaaaagcga aggcgtgctg cccgccatcg aaagcctgcg
     7081 tattccggtg caatatctcg ccaatatgtt gagtgccggc gataccggtc cggtactgcg
     7141 ggcgctgaaa cggatgatgg cgatgcgcca ctatatgcgt tcacaaaccg tggaaggcgt
     7201 tactgatact cgtgccatcg acgaagtagg cctgagcgtc gcccaggtcg aagagatgta
     7261 tcgttacctc gccattgcca actatgaaga tcgttttgtc atcccgacga gccatcggga
     7321 aatggcgggc gatgccttcg cagaacgcaa cggctgcggt tttacctttg gcgacggttg
     7381 ccacggttcg gacagtaaat tcaacctgtt caacagtagc cgtatcgatg ccatcaacat
     7441 caccgaagtg cgcgacaaag cggagggcga ataatgcaga tcctcaaagt gatcggcctg
     7501 ttgatggagt atccggacga gctgttgtgg gaatgcaagg aggacgcgct ggcgttgatc
     7561 cgcagcgacg cgccgatgct tacggatttc acccacaacc tgcttaacgc gccgctgctg
     7621 gataaacagg ccgaatggtg cgaagtgttt gaccgtggac gtaccacatc gctgctgctg
     7681 ttcgaacatg ttcatgccga gtcccgcgat cgcggccagg caatggtgga cctgctggcg
     7741 gagtatgaaa aggtcggcct gcagctggat tgtcgggaac tgcccgatta tctaccgctg
     7801 tatctggagt atttaagcgt actgcctgac gatcaggcga aagaaggatt gctcaatgtt
     7861 gcgcctcctc gccctgcttg gcggtcgctt aaaacaacgc gaggcaccgt ggtacgcgtt
     7921 gtttgatgct ctgctgcaac tggcgggaag ctccctttca agtgacagtg tcacaaaaca
     7981 agtaaacagc gaagagcgcg atgacacccg ccaggcgctt gatgcagtgt gggaagagga
     8041 acaggtgaag tttattgaag ataacgccac ggcgtgtgac agctcgccgc ttaatcaata
     8101 tcagcgacgc tttagccagg atgtcgcgcc gcaatatgtc gacatcagtg cgggaggtgg
     8161 gaaatgattc agtatctgaa cgtctttttt tacgatatct acccgtacat ttgtgcgacg
     8221 gtgtttttcc tcggcagctg gctgcgctac gactacgggc agtacacctg gcgcgcctcc
     8281 tcaagtcaaa tgctcgataa acgcgagatg gtgatatggt cgaatctgtt ccatatcggc
     8341 attttgggga ttttcttcgg gcacttgttc ggcatgttaa cgccgcactg gatgtacgcg
     8401 tggtttttgc cagtggcagc gaaacaacta atggcgatgg tgctcggcgg tatttgcggt
     8461 gtgttgacat tgattggcgg cgcaggactg ctgtggcgca ggctgacaaa tcagcgcgtg
     8521 cgtgcaactt ccactacgcc ggatattatc atcatgagca ttttgctgat ccagtgtctg
     8581 ctggggctaa gtaccatacc gttttcagca cagtatcctg atggtagcga aatgatgaag
     8641 ctggtgggtt gggcgcaaag cattgtcact ttccgtggtg gatcgtcaga aatgcttaac
     8701 ggcgtagcgt tcgttttccg cctgcatctg gtattgggaa tgaccatctt cctgctcttc
     8761 ccgttcacca gattggtgca tgtgtggagc gcgccgtttg agtactttac tcgtcggtat
     8821 cagattgtgc gttcgcggcg ataatttcac cctggccgga tagcccttcg ctctccggcc
     8881 acttgcaggc tatgatagtt ctccagtcac aggagaacgt taatgaaacc gcaggtgtac
     8941 cacgtcgatg cttttacctc tcaaccattt cgcggcaatt ctgccggtgt tgttttcccc
     9001 gccgataatc ttagcgaagc gcaaatgcag cttatcgccc gcgagttagg ccattcggaa
     9061 actgcttttc tgctgcacag cgacgacagc gatgtgcgca tccgctactt tacgccaacg
     9121 gttgaagtgc cgatttgcgg tcacgcgaca gtagctgcgc actatgtacg tgcgaaggtg
     9181 ttaggtttag gaaattgcac gatctggcaa acatcgctgg caggaaaaca tcgcgtgact
     9241 atcgaaaagc acaacgatga ttatcgtatt tcgctggaac aaggtacgcc gggctttgag
     9301 ccaccgctgg aaggtgaaac acgtgaggcg attatcaacg cgcttcatct cactgaagac
     9361 gatattttgc cgggcttgcc aattcaggtc gcaacaacag gacactcaaa agtgatgatc
     9421 ccgttgaaac cggaagtgga tatcgacgcc ctttcgcctg atcttgctgc gctgagcgct
     9481 atcagcaaac agattggctg caatggtttc ttcccgttcc agatccgccc aggcaaaaac
     9541 gaaaccgatg gtcgtatgtt ctcgcctgcg attggtattg tggaagatcc ggtgactggc
     9601 aatgccaatg gcccgatggg tgcatggttg gtacatcaca acgtattgcc ccacgatggc
     9661 aacgtgttat gtgttaaagg ccatcagggg cgcgcattgg ggcgtgacgg catgattgaa
     9721 gtgacggtga caattcgtga taaccaaccg gaaaaagtca ccatttcagg cacagcggtg
     9781 attttgtttc atgccgaatg ggcaattgaa ctctgaaacc gttgcctgat gcgcttcgcc
     9841 tgtcaggtct acaaccgagc taatgtatgc cggataaggc gtttacgccg catccggcaa
     9901 cctgacataa attattttcc cgcctccggg tgcgtatcaa acgccgccat caccagcgct
     9961 aactcatcca cggtaaagcc atgtttcgca tcatccacgc ccagaccaaa ctgttcttgc
    10021 atcacagcat ataatgacgc cacatccggt agatttcgct gctccaccgc gtgcccattt
    10081 tcataatggg taaaatgaaa attggtcagc gttagcttgc caccgtccgg caaatggcgg
    10141 cacatcagca aatgatggcg aaaatgcgac tgcggccagt gcgccgacca gaaattgccc
    10201 atcacataat cgctttgttg ctgctcgcag agatcaaaac ggtacatcga ctgccagtgc
    10261 tgatgatgat taaactgcaa cacccaatca tcaccctcct gcaacaaccg ataatctccg
    10321 tgtggcgtgg tctgcacgag atcggaaact aaacgaatcg gcgcggttag cgtctgccca
    10381 ccgaaaccga catcagcaat ccatttttcc tcttccagtt ccaccaacag caaacgatgg
    10441 gtgcgcggcg gtaatgctgg cggatttgat aacactacgc gccctaacaa gctgcgaacg
    10501 ttaaactcca gctcgcgtaa cacccgctca aacacgccat tctgctcaaa acagtaaccg
    10561 ccacgacggg ctatcaccag tttctcttcc agcgattgat catcaagctg tatttccctc
    10621 ggcagcaaaa cgtcgaggtt ttcaaacgga atagtgcaat tgtgtttcag gtgcaatgca
    10681 cgaagcgtat caatattgac cgcagcagct cccgaccagt taatacgggc aaaatagtga
    10741 ttcagaatgg gcgtcatcag gcgtttccct tagtggtttt ctcaacttat aacctggaat
    10801 agacgccgca ccggctattt tgtgcgatcg cggacatgca tcacgtttgc aaatcgccaa
    10861 tcccgttaat ttcgttactt attgcgttac ggactacgct taaaaaaacc ttcttcgcga
    10921 ggcgatacca tgagccgatt catccccata gaattacacc atgccagccg tctgttaaat
    10981 catggcccaa ctgtcatgat caccagtttc gatgagcaat cccagcggcg taacattatg
    11041 gcagcagcct ggtcaatgcc ggtggagttt gaaccgccac gcgtggcgat tgtggtagat
    11101 aaatcgacat ggaccaggga gttgattgaa cgtaacggta aatttggcat cgttatcccg
    11161 ggcgttgcag caactaactg gacgtgggca gtgggaagcg tgtcgggtcg tgaagaagat
    11221 aaatttaatt gctatggcat tccggtggtg aaaggtccgg tgcttggttt gccacttgtt
    11281 gaagagaaat gcctggcgtg gatggagtgc cgcctgctac ctgcgacttc tgcgcaacaa
    11341 aaatacgaca cgctgtttgg tgaggtggta tcagcagcgg cagacgcacg ggtatttgtc
    11401 gaaggccgct ggcagtttga tgatgataag ctcaatacgt tgcatcattt aggtgctggc
    11461 acgtttgtta ccagcggcaa gcgagttacg gcgggttaag cattacatgc tgtaaccggg
    11521 tttctttatc aaagcctcca tttggggcgc gatttcggca tcccagatag cttgccaaga
    11581 ttctggttga atctgctgta gagcaatgct tatcgagcta tctttactgt tcagatggcg
    11641 aataataacg tcggtaatat ctgcagcaag tgctgctttt tattgttcgt ccagttcacg
    11701 cggaaaacat ttaatgtcga tgtgcggcat gattgtgctc cttttgaaag atgtcagtaa
    11761 aattaacaca cattgtgaat gtcgtgtgtc cggtaatgcg tttttacgac aataatgcca
    11821 gggcaagatt acgaaagcct
//
LOCUS       DAZFWL020000139        11803 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFWL020000139 DAZFWL020000000
VERSION     DAZFWL020000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.139"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2345)
                     /locus_tag="ACHKNP_RS20180"
                     /old_locus_tag="pgaptmp_004036"
     CDS             complement(<1..2345)
                     /locus_tag="ACHKNP_RS20180"
                     /old_locus_tag="pgaptmp_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4706107.1"
                     /translation="MNDVTVVTSVTYPSPESLALVADVQYHEPYLSAALNRKFRGIVD
                     PGFYAGFLPKPGGGMNLLITSVDGDKTAGAASVDIGEFYQVTIQQRTDIPLALSAGKK
                     YAIVLKGRYLLGEDTYQVNTASHIHAAEFVTRTYTDSYQLGDGELLVCTVNIPAGVSA
                     ITQEMIDTSERINRTIGIDISDSVTSSRSDVAASSLAVKKAYDLANSKYTAQDASTTQ
                     KGLVQLSSETNSDSETMAATPKAVKSVKDLADTKAPIESPSLTGTPTAPTAAQGTNST
                     QIANTAFVKAAITALINGAPGTLDTLKEIAAAINNDPNFSTTINNALALKAPLASPAL
                     TGIPTAPTAAQGTNNTQIATTAYVRAAISALVGSSPEALDTLNELAAALGNDPNFATT
                     MTNALAGKQPLDATLTALAGLATGANKLPYFTGTDTISQTDLTSVGRDILAKTSVLAV
                     IQYLDLRELGTSGEKIPLLSTANKWSARQTFNGGITGALTGNADTATKLKTAININGV
                     RFDGSADININTLVSRGRVTALEANAQGTSGIQLYEAYNNGYPSPYGNVLHLKGATAA
                     GEGELFIGWSGTSGDHAPVHIRSRRDTDSANWSEWAQVYTSKDSIPGVNAKGDQDTSG
                     NAATATKLQTACTINGVSFDGSTDITLTAAHVAAFARRATDTYADADGGVPWNAESGA
                     YNVIRSADSYILVNFYTGVGSCPTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSK
                     NLPPESYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAA"
     gene            complement(2357..2791)
                     /locus_tag="ACHKNP_RS20185"
                     /old_locus_tag="pgaptmp_004037"
     CDS             complement(2357..2791)
                     /locus_tag="ACHKNP_RS20185"
                     /old_locus_tag="pgaptmp_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4706108.1"
                     /translation="MSDVSTNLYKSQLLDYYYQRRAESSINKGSRFLISKAVFGTSSL
                     VTKKGDGTYEIGELPKAFDLAELTSQFCTINLVPTYSGGIITVRMDLDQSQLQEGKNY
                     PFNTLVVLDNENKPIAIICVQEDSLYVGKTYTAVMAINTTTA"
     gene            complement(2870..3706)
                     /locus_tag="ACHKNP_RS20190"
                     /old_locus_tag="pgaptmp_004038"
     CDS             complement(2870..3706)
                     /locus_tag="ACHKNP_RS20190"
                     /old_locus_tag="pgaptmp_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4706109.1"
                     /translation="MQRSWFNNRLTSAKQKSLLYKSLADLVQSMMDTFVDPWLERITN
                     RKSIFSMSKEDLETRTNELGQFFTIRTSDSSSVPMLLQQRLDEIHFKGTERPINQTIY
                     REFNGISVLWDPIYAPVDLERHPYGTVLIPESTLETTGGTFGEMFLTSRGMISIPIND
                     LARTMGITGTIDQSAITEEILRKFNQFVKPLLPLHIVFDGLTLYLSVVVNEQADMITL
                     NEISDTEKAFCWFETSDTTSLTGVTSISAPITATPGGTIVKATPTFDRTRADDLLLDS
                     DA"
     gene            complement(3706..5139)
                     /locus_tag="ACHKNP_RS20195"
                     /old_locus_tag="pgaptmp_004039"
     CDS             complement(3706..5139)
                     /locus_tag="ACHKNP_RS20195"
                     /old_locus_tag="pgaptmp_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin hydrolase"
                     /protein_id="HGU4706110.1"
                     /translation="MSKTTPTKDSIRAEFEELVEKDSFWSKFVGSQFVSMLTLFITQI
                     VYRCFQYADAALAEGFISTATRRSSILAAAETNSYVGTKPTPSSGMIEITATSEDAPA
                     VIPKNMPLISDDQYPYMTMDVCRLVDGTGTVEVAQLEIQEVTYTVTAAKEFLEVVLSK
                     ALTAVCYKLEVFVTTDGKTTQWSSSTMFRLAGSKSQVYVEFYKPSEQLGVRFGDGLIG
                     QIPPEGSTITLKVWCTNGDITLVAGQNLTPVDSAANLANLISVKTTTPITAGTDAETT
                     EITRNRAQYYLAYDDQVVWGGDYTYFLVRNIPGLSWVKAWGEGQQEKLDGAYNVHNIN
                     KIFISGWHPNKSQSELEEMILTAFKKVPNELNKKFSYKEVRKLPFKITITGRISASLT
                     IENVTDELKSALETKFGRDSTFFDPNRVGKYILIKKKDVWAYIETLGYFRDFYLEFVE
                     WNESNGFYDFVYLDTESSTFNISYEEE"
     gene            complement(5136..5492)
                     /locus_tag="ACHKNP_RS20200"
                     /old_locus_tag="pgaptmp_004040"
     CDS             complement(5136..5492)
                     /locus_tag="ACHKNP_RS20200"
                     /old_locus_tag="pgaptmp_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706111.1"
                     /translation="MANNNEIDPLLTLELSGVKTYESQEEAWGARLYEWLNTYQGEVY
                     GDPSWGNVLPQFKHEPTNLSHVQIAVEAMLLQKLTVDLPDIPISGLSVAEGDAFDKLK
                     ISIRIRDITITQDVVL"
     gene            complement(5492..8884)
                     /locus_tag="ACHKNP_RS20205"
                     /old_locus_tag="pgaptmp_004041"
     CDS             complement(5492..8884)
                     /locus_tag="ACHKNP_RS20205"
                     /old_locus_tag="pgaptmp_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HGU4706112.1"
                     /translation="MERKNANIDDVIRTVETASAKELEELAGIREAVEDLKGGRVATV
                     DPVSRSVSALNRTIENSRPDFVANAPSVDPIVEAMKRLNLGDVSRVVQEDVAQQEQRA
                     KSTTPKGKKRRRKAIPEDVKAQRTEAAEHAREMFGQKGGAQKSQNQRDARGRFIGKSG
                     SKAAAEDARAERAEKDRRKEDDERLNAESGLLKKLSKVAEGIGNPSETRAVDALGYAV
                     AGPLWAAGKELGGISKEVGGSLNGARKSIADVIRGNDDNSRRKGFFRRKSQNSADVVQ
                     VNTQKRTVQELQEQTSEIKEGNDKILRALDQIAKNTGKKKGGLLSKLFSLLGKGAGGV
                     ASLLMGRGMLKKAGALAFGALGAKKLVGMLRGGGKKTLAHEGGDLAARAAGKLGLKAV
                     GKGALRAIPLVGTVAGGIYDAVTGWNDTEAQRRAFGLKSGQDPSFQQKAAYTLANVLD
                     MGGLVSGISSAIGDVLKSLGFEDIGNMLQSFSTESIAQAIDSGVTNLETYISNLGDTI
                     STKFDDYTAKIGDAVSAWFSDTSNKLLEKLDAIKDFFTVDNLKQVFSDAIDSAIDFIK
                     NPGKHIKEAAGNIWDGVKSLPGKALDAAVDAVKNTPAAMIVSKIPNPIGEANAKEITP
                     ELKAPVNSHQETSDSKTESDAKQTNIATRVINAALDTAKDSNKTVKETANQIINANAV
                     ETGNSAVRKIDSAIGQNSSSSSSRNTTGTRNDIQKAADTYNNGNLDVKVGSLGAEGKA
                     NLDKLAPYFAELENKYGLPEGTLYAIAATESGGNPYAKSQTGALGMFQFTGIAREETG
                     LAEGESFDPVKSAEAAALLMSKYLKQANGDLNEAITAYNAGFGTINKWKKGTGDLSKE
                     NREYAIKVNTHRARYLGGEIYTPGAGAQGGAQYGVREPLPDNAVIDQSTGLAFTPGDS
                     PFEKGGLVDKIGNAVGVNDLVNKFMNGRGMRREVVQGTLEERARGKGTATAAGNVYVD
                     TPMPVEEARPVASNSSYFDQLGAQMGIDGLFDKLRNSPGMRKNNAPEPASTSQVTTAV
                     NDLQQPTGRMQIDGQVISDLGGSGAKPTMQLADNTVSLDGETKRLFAQMTSLLARIEE
                     HTKDSAKGQGTVVKVSTPQPGVMRTVPLSIDDPLMNDYARVD"
     gene            complement(8966..9847)
                     /locus_tag="ACHKNP_RS20210"
                     /old_locus_tag="pgaptmp_004042"
     CDS             complement(8966..9847)
                     /locus_tag="ACHKNP_RS20210"
                     /old_locus_tag="pgaptmp_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein"
                     /protein_id="HGU4706113.1"
                     /translation="MLLPLFPLPSRPTELIQFRQPNIADAMRFNSITPEEQEQQTTAY
                     LKALLAEPAKHDPLTWTAQDRITALWWIFTGSRETPVETFTYTCKHCGKEHYYDCDMN
                     ALAEDIQVLEVEPFIDDIEVSVEGVPYQWRIVPLDGWAMEMLEMRRAALPPEDDAEFK
                     EAIVDLRFWEFAYQCELYNDVSGTREDQAERRYETIKRMAIDTEFMKLAAHIRLAHEK
                     LEHGLPCYIDKGEMRLRLPPHKCPNQDTKESTEGAYTRLWVPFRATDFIPQVGIEKLS
                     DLSVQPGFVWGYTDSGR"
     gene            complement(9862..10473)
                     /locus_tag="ACHKNP_RS20215"
                     /old_locus_tag="pgaptmp_004043"
     CDS             complement(9862..10473)
                     /locus_tag="ACHKNP_RS20215"
                     /old_locus_tag="pgaptmp_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4706114.1"
                     /translation="MGFNVASVKSYVSSALTTTLFGSGVGEREVGKLTSIIMNKMLFA
                     QGWQFSVEVDGLEGADFFAKDITYHDYSIEYETIKIGGGNILQPTERSPGQITMMVRD
                     TVDGLVLDWFKTAKSRVINPDGTGNIPSKYLLNVRIYRLLSSGLTKLENEMTVFPVTT
                     GDVTYARDQVTEFKSFPMTFALHSTFNQSSSSLASLLGFSFSL"
     gene            complement(10484..11050)
                     /locus_tag="ACHKNP_RS20220"
                     /old_locus_tag="pgaptmp_004044"
     CDS             complement(10484..11050)
                     /locus_tag="ACHKNP_RS20220"
                     /old_locus_tag="pgaptmp_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706115.1"
                     /translation="MTPRQLLEDVKSRFTPLIADEPALLESLLRKALGTYQDRAGHIK
                     RIRITDQASKSLACPVDFLALVSVTDHTGDLVYSDVYDGNIELEDTHRAVYPLNVSYL
                     ANLRDMDLDNGDVPPEIIGLLSDYLEVLIAIPNTDRLRRISIAGKLDASNLSDENTLY
                     QRKLDLEEKMSATRAIIPGIVLFSSMLK"
     gene            11281..>11803
                     /locus_tag="ACHKNP_RS20225"
                     /old_locus_tag="pgaptmp_004045"
     CDS             11281..>11803
                     /locus_tag="ACHKNP_RS20225"
                     /old_locus_tag="pgaptmp_004045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706116.1"
                     /translation="MHSLQKYIKHKYTHLTFFLSLLFFMIFESREDIIKKTLSFSLDE
                     VLPFLMFAFFSAFISITIKEKIAKKYPETNDGITIIALIFGVTLYSVFILYYLYGDVY
                     QSTIALLISSVLLGSGWWVQATVSKVAARKSHTLNTLMNQRNSELFHKKVSSVANTFG
                     LRKTINEIIAKQRL"
BASE COUNT         2958 a         2946 c         2589 g         3310 t
ORIGIN      
        1 gctgcaagtt tcgggtatgc agatttgtca aaagtctgcc cctgcatcag ggcataacca
       61 gacggaacgg tatctgatgg ccacgggatt ggtgcgccga ctgggtagct ttctggtgga
      121 agatttttcg aggtataaac ttctgcccag tcttcctcaa aaccataacc gtctcttgaa
      181 gaacggtaga acagaccacc atttctgtaa tgcgccttca tctgcaaggt cgggcaactt
      241 ccgactccgg tatagaagtt aaccagaata tagctgtcgg cagagcgaat gacattgtaa
      301 gcgcctgatt cggcattcca gggaacgccc ccatccgcat cggcatacgt atccgttgct
      361 cttctggcaa aagcagcaac atgcgcagcg gttaaagtga tatcagtaga accatcaaac
      421 gagacgccat tgatagtaca tgctgtctgc aacttggtcg ctgtagccgc attaccagag
      481 gtgtcctgat cccctttggc attgacgccg ggaattgaat cttttgacgt atagacctgc
      541 gcccattcag accagttggc agaatcagta tcacgccgcg aacggatatg tacgggcgca
      601 tggtcaccgc tcgtgccact ccagccaatg aataactcac cttcgccagc agcggtggca
      661 cctttaaggt gaagcacatt gccatagggg gaagggtagc cattgttgta tgcctcatac
      721 agctgaatcc cggatgttcc ctgtgcattc gcctctaggg ccgttacgcg accgcgagat
      781 accagagtat tgatattaat gtcagccgaa ccatcgaatc tgacgccatt aatgtttatg
      841 gctgttttta atttcgtcgc ggtgtcggcg ttccctgtca gcgccccggt gatcccaccg
      901 ttgaaagtct ggcgtgcact ccatttgtta gccgtgctca acagggggat cttttcaccg
      961 cttgtgccaa gttctcttaa atcaaggtat tggataacag caaggacgct tgttttggcc
     1021 agaatatcgc gaccgactga cgttaagtca gtctgagaaa tagtgtctgt accggtaaag
     1081 tacggcaatt tatttgcgcc tgtagcaaga ccagcaagcg cggttaaagt tgcatccaga
     1141 ggctgtttgc ctgccagcgc atttgtcatt gttgtcgcaa agttcgggtc attaccaagt
     1201 gctgcggcaa gctcattcag ggtatcaaga gcttctggtg atgaaccaac caatgcggat
     1261 atggcagctc ttacataagc ggtcgtagca atctgcgtgt tattcgtacc ctgtgcagcg
     1321 gtaggcgcag taggtattcc cgttaatgca ggacttgcta aaggagcttt aagagccaga
     1381 gcattgttga tagttgtgct gaaattcggg tcgttattga tcgcagcagc tatttcttta
     1441 agcgtatcca gtgtgccagg tgcaccgttg ataagtgccg ttatagctgc cttaacaaag
     1501 gctgtatttg cgatctgcgt gctatttgta ccttgcgctg ccgtcggcgc ggttggcgtt
     1561 cctgtcagac tcgggctttc tattggcgct ttggtatcag caagatcttt tacagactta
     1621 acggctttag gggtagccgc cattgtttcg ctgtcgctgt tagtttcgct actgagctga
     1681 actaatccct tttgcgttgt gcttgcatcc tgcgccgtat acttactgtt cgccagatcg
     1741 taggcttttt taactgccag cgaacttgca gcaacatcac ttctgctact ggttacagag
     1801 tctgaaatat caatgccgat cgtgcggtta atacgctcgg atgtatcaat catctcctgg
     1861 gtaatggctg atactccagc agggatattc accgtacaaa caagcagctc cccatctcct
     1921 aactgatatg aatcggtata ggttctggta acaaattcag ccgcatgaat atgtgacgcg
     1981 gtattaacct gataggtatc ctctccaagg aggtatcttc ccttcagcac aattgcatat
     2041 ttcttgcctg cactaagtgc aagaggaata tccgtacgtt gctgaatagt tacctggtag
     2101 aattcaccaa tatccactga cgctgcgcca gcagttttat caccatccac agaggtgatt
     2161 aacaggttca tcccaccgcc aggcttaggt aagaaaccgg cataaaatcc cgggtcaaca
     2221 atccccctga attttcggtt tagcgcggct gacagatatg gttcgtggta ttgcacatca
     2281 gccaccagag ccaacgactc gggtgatggg taagtaactg atgtaacaac tgtaacgtca
     2341 ttcatcaagc atatccttat gctgttgtcg tgtttatggc cataactgcg gtatatgttt
     2401 tgcccacata caacgagtct tcctggacac aaataatggc gattggcttg ttctcgttat
     2461 ccagaacaac cagagtgttg aatgggtagt ttttcccttc ctgcaactgg ctttgatcaa
     2521 ggtccattcg gacagtaatt atcccacctg agtaggttgg cacgaggttg atggtacaaa
     2581 attggctggt cagttctgcc agatcgaaag cctttggcag ttctccaatc tcataagtgc
     2641 catctccttt cttagtaacc agagaactgg taccgaaaac ggccttgctg attaaaaatc
     2701 gagagccttt gttaatggac gattcagcgc gccgctgata gtaatagtcc aacaactgac
     2761 tcttatagag gtttgttgag acgtcagaca tgattttccc taatcactgt tgtgaagcct
     2821 cattgtaaga gaagtaactt gtcaccccgc cctgcggacg gggtgattgt caggcgtcgc
     2881 tatccagcaa caaatcatct gcgcgggtgc gatcaaacgt aggtgtcgct ttcacaatag
     2941 tgccaccagg cgttgcggtg atcggggcgc taatcgacgt aactccagta agcgaagttg
     3001 tatccgaagt ttcaaaccag cagaatgctt tttcggtatc agaaatctcg ttcaaagtga
     3061 tcatgtcggc ctgttcattt acaacaaccg acaaatagag cgtaagccca tcaaacacta
     3121 tatgcagtgg cagtagaggc tttacgaact gattaaactt tctgagaatt tcttctgtaa
     3181 ttgcggactg atctatcgtg ccagtaatac ccattgtccg ggccaggtcg tttatgggaa
     3241 tactgatcat ccctctggaa gtcagaaaca tctcgccgaa tgtgccgccg gtagtctcca
     3301 atgtgctttc tggtattaga actgtgccat agggatgacg ctcaaggtcc accggtgcat
     3361 atatcggatc ccataaaaca gaaataccgt taaattcgcg gtaaattgtc tggtttatag
     3421 gacgttcagt tcccttaaag tggatctcat caagccgctg ttgtaacaac atcggaacgg
     3481 aagatgagtc cgaggttctg atagtaaaga actggccaag ttcatttgtc ctggtctcca
     3541 gatcctcctt gctcatggag aaaatagact tccggttggt aattcgctcc aaccatgggt
     3601 caacaaaggt gtccatcatt gactgaacca aatcagccaa tgatttatag agcaatgact
     3661 tttgcttagc tgatgtaagc cggttattaa accaggaacg ctgcatcact cctcctcata
     3721 cgaaatatta aaggtggagc tttctgtatc cagataaacg aaatcgtaaa agccgttgga
     3781 ctcattccac tcgacaaatt ccagataaaa gtcgcggaaa taacccagcg tttcgatata
     3841 cgcccaaaca tcttttttct taatcaggat gtactttccg acacggttcg gatcaaagaa
     3901 agttgagtca cgcccaaatt ttgtttccag tgccgacttc agctcatcag tcacgttctc
     3961 aatggtcagg cttgccgata tccgcccagt gatggtgatc ttaaagggta gttttctgac
     4021 ctctttatac gagaatttct tgttcaactc attcggcacc ttcttaaagg cggtcaggat
     4081 catttcttca agctctgact gacttttatt cggatgccat cctgaaataa atatcttatt
     4141 gatattatga acattataag caccatctaa tttttcttgc tgaccctcgc cccatgcctt
     4201 tacccaggac agtcccggga tgttacgcac cagaaaatac gtatagtccc cgccccatac
     4261 gacctgatca tcataggcaa ggtaatattg tgcacggtta cgtgtgatct ccgttgtttc
     4321 agcatcagta cctgcggtta taggtgtcgt tgtcttaact gaaatcaaat tagctaaatt
     4381 agccgcagaa tcgacaggcg tcaggttttg gccagcaacc agggttatat cgccgttggt
     4441 gcaccatacc ttaagcgtaa ttgtcgaacc ttctggcggt atttgcccaa ttagcccatc
     4501 gccgaatcga acccccaact gctcggatgg tttataaaac tcaacgtaga cctggctttt
     4561 actaccggct aaccggaaca tagtgctgga agaccactgc gtggtcttac catcggtcgt
     4621 cacgaatact tccagcttat agcagacagc agtgagagcc tttgataaca cgacttccag
     4681 aaattctttg gctgccgtaa cggtatatgt cacctcctgg atttccaact gtgccacttc
     4741 taccgtaccg gtgccgtcaa ccaacctgca tacatccata gtcatgtaag ggtactggtc
     4801 gtcagatatt aaaggcatgt ttttggggat taccgctggg gcatcttcac ttgtggcggt
     4861 gatctcaatc atccccgatg acggtgttgg cttggtacca acgtaactat tcgtttctgc
     4921 cgctgccagg atagaggaac gccgcgtcgc ggtcgatata aagccttcag ccagcgccgc
     4981 atcggcatac tgaaagcacc tgtagacaat ctgggtaata aacaatgtca gcatcgagac
     5041 aaattgagag ccgacaaact tcgaccagaa tgaatctttc tcgacaagct cttcaaactc
     5101 tgcacgaata ctgtctttag tcggtgttgt tttactcata gcaccacgtc ctgtgtgata
     5161 gttatatccc tgatacgaat ggatattttc aacttatcaa aagcatctcc ctcggctact
     5221 gacaagccag aaatcggtat gtcaggtaaa tctaccgtca gtttttgcaa cagcattgcc
     5281 tcaaccgcaa tttgaacatg cgacaagttg gtcggttcgt gtttaaactg cggtaaaaca
     5341 ttgccccatg acggatcccc gtatacctca ccctgataag tgtttagcca ctcatataaa
     5401 cgagcgcccc aggcctcctc ctgggactca tacgttttta cgccggataa ctccagcgtc
     5461 agcaaaggat caatttcgtt attgttggcc atcaatcaac tctcgcgtag tcattcatca
     5521 acggatcatc aattgacagt ggtaccgtgc gcataacgcc cggctgaggc gtgctgacct
     5581 ttacgacagt tccctggcct ttcgccgagt ctttggtgtg ctcttcaatc ctggcaagca
     5641 atgaggtcat ctgcgcaaac agccgcttcg tttcaccatc aagtgaaacg gtattatcag
     5701 ccaactgcat tgtcggcttg gcaccggagc cgccaaggtc actaataacc tgtccgtcta
     5761 tctgcatacg accggttggt tgctgcaaat cgttgacggc agtcgtcacc tgggacgtgg
     5821 aggctggttc aggcgcatta tttttccgca tccccggcga gttgcggagt ttatcgaata
     5881 gtccatcaat ccccatttgt gcgccgagtt ggtcaaagta acttgagttg ctggccaccg
     5941 gacgcgcctc ttcaactggc atcggagtat caacatacac attgccagct gctgttgcgg
     6001 tccccttccc tcgtgcacgt tcttcgagcg ttccctgaac gacttcccga cgcatacccc
     6061 ggccattcat gaatttgttg accagatcgt taacgccaac agcattgccg attttgtcta
     6121 ccaaaccgcc tttctcaaac gggctatcac caggggtaaa cgccagacca gtagactgat
     6181 cgataacggc gttatcaggt agtggttccc tcactccata ttgcgcccca ccctgtgctc
     6241 ctgccccagg tgtatagatt tcaccaccta aatagcgagc acgatgagta ttgaccttga
     6301 tcgcgtactc acggttttcc ttcgataagt caccggtgcc ttttttccac ttattaatag
     6361 tgccaaaccc agcgttatat gcagtgatgg cctcgtttaa gtctccattg gcttgcttca
     6421 gatacttgct catgagaaga gccgcagctt ctgccgattt cacaggatca aacgattcac
     6481 cttcagctaa gccagtctct tcacgagcaa tgcccgtgaa ctgaaacatt cctagagcac
     6541 cagtttggga ttttgcgtac ggattaccac cagattcagt tgcagcaatc gcgtaaagag
     6601 tgccttcagg aagaccatat ttattctcca gttcagcaaa atacggagct aacttatcga
     6661 gatttgcctt accttcagcg ccaaggcttc cgacttttac atctaagttg ccattattgt
     6721 aggtatcagc agctttctga atgtcattcc tggtgccggt ggtattacgc gacgatgatg
     6781 acgagctatt ttgtccaata gctgaatcaa ttttccgcac cgcgctattg cccgtttcta
     6841 cggcatttgc attgataatc tgattggcag tttctttaac tgttttattg ctatctttcg
     6901 ccgtgtccag tgccgcattt atcacgcggg tagcaatatt agtctgttta gcatcggatt
     6961 cagttttaga atcagatgtc tcctggtggc tattaaccgg agcttttaac tctggagtga
     7021 tttcttttgc attagcctcg ccgatcggat tgggtatttt tgatacaatc attgccgcag
     7081 gggtattttt aacggcatca accgctgcat ctaatgcttt accgggtaaa cttttaaccc
     7141 catcccaaat attaccagcc gcctctttaa tgtgttttcc tgggttctta atgaagtcaa
     7201 ttgcactatc aattgcatca ctgaaaacct gtttcaggtt atcgacagta aagaagtctt
     7261 ttatggcatc cagcttttca agcaacttat tagatgtatc gctaaaccat gctgaaacag
     7321 catcaccaat ctttgctgtg taatcatcga acttggtaga aatggtgtcg ccaaggttag
     7381 aaatatatgt ttctaagttg gtaaccccac tatcaatggc ctgggcaata ctttccgtcg
     7441 aaaatgattg caacatattg ccgatatcct caaatccaag tgatttgaga acatcaccaa
     7501 tagcactgct aataccagat accagtcccc ccatatcgag aacattggct aacgtataag
     7561 cggctttttg ctggaatgat ggatcttgtc ctgatttaag cccaaacgct cgacgttgcg
     7621 cttctgtatc attccaaccg gttaccgcat cataaatacc tccagccact gtgccgacta
     7681 ggggaattgc gcgtaacgcc cctttaccaa ctgcctttaa tccaagttta cctgctgccc
     7741 gggcagccaa atctccgcct tcatgggcga gagtcttctt gccaccaccg cgtagcattc
     7801 ctacaagttt ctttgccccc agagcgccaa aagcgagtgc tccagctttt ttcagcatgc
     7861 cacgccccat taacaacgac gcgacgccac cggccccctt ccctaacagg ctaaatagtt
     7921 tggacagcaa gccgcccttc tttttcccgg tgtttttggc tatctgatca agggcgcgga
     7981 gaatcttgtc attgccctct ttaatttcgc tggtctgctc ctgaagttcc tgaaccgtcc
     8041 gtttttgggt gttaacctga acgacatcgg cactattttg cgatttacgc ctaaaaaaac
     8101 cttttctacg gctgttatcg tcattgccac gaatcacatc ggcaatagac tttctagcac
     8161 cattaagcga tccaccaact tcttttgata tcccgccaag ctccttccct gcggcccaca
     8221 atggaccagc aacggcataa cctaacgcat cgacggcacg agtctctgaa gggttaccta
     8281 tgccttcagc tacttttgac agttttttta ataaacctga ttcagcattt agacgctcat
     8341 catcctcttt gcgcctgtcc ttttcagcac gttcagcacg ggcatcttcc gctgcggcct
     8401 tactccctga ctttccaata aaacgaccac gcgcatcgcg ttggttttgg cttttttgcg
     8461 caccgccttt ttgaccgaac atttcgcgag cgtgttcggc tgcttcggtc cgttgtgcct
     8521 ttacatcctc tggtatagcc ttcctgcgtc gttttttacc ctttggtgtg gttgatttgg
     8581 cccgctgttc ctgttgagca acatcttcct gaactacacg agaaacgtcc cctaaattaa
     8641 gccgtttcat tgcctcaaca atagggtcca ctgatggcgc attggccaca aagtctggcc
     8701 gggaattttc gattgtgcga tttaatgccg acacgctgcg agagacagga tcaacagtag
     8761 caactcgccc acctttcaaa tcttcaacag cttcccggat accagcaagc tcttccagct
     8821 cttttgcgct ggcggtttca actgtcctta tcacatcgtc aatgttggcg ttttttcttt
     8881 ccatgatctt atcgcctacc gtttcggttt aagtttttct tccagtttct ccaacaggaa
     8941 aaacgcatag gattcagtaa gcctttcagc gtcctgaatc ggtatacccc catacaaaac
     9001 caggttggac actaaggtct gatagctttt caatccccac ctgtggaatg aagtcggtag
     9061 cccgaaaggg cacccacaga cgggtatacg caccctctgt ggactccttt gtatcctggt
     9121 ttgggcattt gtgcggcggg agacgaagac gcatttcgcc tttatcgatg tagcacggta
     9181 aaccatgttc gagcttttca tgagccagtc ggatgtgtgc cgccagcttc ataaattcag
     9241 tatcaatggc catccgttta atcgtttcat aacgacgctc agcctgatct tcacgagtac
     9301 cactaacatc gttataaagc tcacactgat aagcgaattc ccaaaaacgc aaatcaacga
     9361 tcgcttcttt gaattccgcg tcgtcttcag gtggcaatgc tgcacggcgc atctccagca
     9421 tttccattgc ccaaccatca agcggcacga tacgccattg ataaggcact ccctctacag
     9481 acacctcaat atcgtcaatg aacggttcca cttccaggac ctggatatct tcagccagag
     9541 cattcatatc gcaatcgtaa taatgctctt taccgcaatg tttacaggtg taggtgaatg
     9601 tctcgaccgg tgtttcacgg gagccggtaa atatccacca taacgcggta atccggtcct
     9661 gcgccgtcca tgtcagggga tcatgtttcg cgggttcagc cagcaaggct tttaaatacg
     9721 ccgttgtctg ttgttcttgt tcctccggtg ttatcgagtt gaaacgcatc gcatcagcaa
     9781 tatttggctg acggaactgg atcaattcag ttggccgcga tggtagcggg aaaaggggta
     9841 aaagcatcct tgctccttaa ttcaaagaga aaaactaaag cccagaaggg aagccaaaga
     9901 actcgaggat tggttaaacg tgctgtgcaa tgcgaaggtc attgggaatg acttaaattc
     9961 cgtaacctga tcccgcgcat aggtgacatc gccggtagtg acagggaata cagtcatctc
    10021 attttccagt ttggttaagc cggaagacag caaccgataa atacgcacat taagcaaata
    10081 tttagacggt atattcccgg taccgtctgg attaatcacc cgactttttg ccgtcttaaa
    10141 ccagtccaaa acgaggccat caacggtatc cctgaccatc attgttatct gccctggcga
    10201 acgctccgtt ggctgaagga tattccctcc gccgatttta atcgtttcat attcgatgct
    10261 gtaatcgtgg taggtaatat ctttggcaaa gaagtctgcc ccctccagtc catcaacttc
    10321 gacagagaac tgccatcctt gcgcgaacaa cattttgttc atgatgattg acgtcagctt
    10381 accgacttcc cgctcaccaa cgccggagcc aaataatgtc gtcgttaatg ccgaagatac
    10441 ataagacttt actgaagcaa cattaaaccc catatcagcc ctctcacttc aacatggatg
    10501 agaaaagaac aattcccggg ataattgccc ttgttgcgct cattttctct tccagatcca
    10561 gctttcgctg atacagcgtg ttctcgtcgg ataaattgct ggcatcgagt ttccccgcga
    10621 tagatattct tcgcaggcga tcagtgttgg gtattgcgat tagcacttcc agatagtcag
    10681 aaagtaaccc aatgatttca ggtggcacat ccccattatc cagatccata tcacgcaaat
    10741 tagccagata tgacacattc agtgggtata ccgctcgatg ggtatcttca agctcgatat
    10801 tcccatcgta aacatcggag tagacaagat cgccggtgtg atctgtaacc gatacgagcg
    10861 caagaaaatc aactgggcaa gcaagtgatt tactggcctg atcggtgatg cgtatccgct
    10921 tgatgtgccc cgccctatcc tggtaggttc ccaatgcttt tcttagcagg gattccagta
    10981 aagcaggttc atccgcaatc aaaggtgtga agcgggattt gacatcttcg agtaattgtc
    11041 gtggtgtcat tgaaacctcg tagaatctgg tgtgttaacc gattctacga gtagtcattt
    11101 gtccattgag tgggaatatt gccgtccggg agtaatatgg tatcattaag ttagagtagg
    11161 tagtgtagat ggaatgagga gtatcatatg gttatcacac aatcaggttg ctttgagtca
    11221 ggttgttttg agtaaaacaa taaaacgccc tttaatgggc gtattttttt aggatttaaa
    11281 atgcattctt tacagaaata cataaaacat aaatatactc acttaacttt ttttctctca
    11341 ttactttttt tcatgatatt tgaatctcga gaagatatta tcaagaaaac attatctttt
    11401 tctctcgatg aagttttacc attcttaatg tttgcgttct tctccgcctt tatcagcata
    11461 acgataaagg aaaagatagc aaaaaaatac ccagaaacaa acgatgggat taccattata
    11521 gctttaatct ttggtgtaac gctatatagt gtatttatat tatattattt atatggtgat
    11581 gtataccaat ccacaattgc tttacttata agttctgtgc tacttggttc tgggtggtgg
    11641 gttcaggcta ctgtgagtaa agtagccgca agaaaatcac acaccttaaa tacactaatg
    11701 aatcaaagaa acagtgaatt atttcacaaa aaagtctcat cggtagcgaa tacgttcggg
    11761 cttagaaaaa ccataaatga aattattgcc aaacaaagac tta
//
LOCUS       DAZFWL020000140        11752 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFWL020000140 DAZFWL020000000
VERSION     DAZFWL020000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.140"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..677)
                     /locus_tag="ACHKNP_RS20230"
                     /old_locus_tag="pgaptmp_004046"
     CDS             complement(<1..677)
                     /locus_tag="ACHKNP_RS20230"
                     /old_locus_tag="pgaptmp_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU4706117.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFS"
     gene            685..1116
                     /gene="tnpA"
                     /locus_tag="ACHKNP_RS20235"
                     /old_locus_tag="pgaptmp_004047"
     CDS             685..1116
                     /gene="tnpA"
                     /locus_tag="ACHKNP_RS20235"
                     /old_locus_tag="pgaptmp_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HGU4706118.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRLQIFDHDATEK
                     LRTYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(1132..1320)
                     /locus_tag="ACHKNP_RS20240"
                     /old_locus_tag="pgaptmp_004048"
     CDS             complement(1132..1320)
                     /locus_tag="ACHKNP_RS20240"
                     /old_locus_tag="pgaptmp_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HGU4706119.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(1324..1683)
                     /gene="yeaR"
                     /locus_tag="ACHKNP_RS20245"
                     /old_locus_tag="pgaptmp_004049"
     CDS             complement(1324..1683)
                     /gene="yeaR"
                     /locus_tag="ACHKNP_RS20245"
                     /old_locus_tag="pgaptmp_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HGU4706120.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1856..2494)
                     /gene="leuE"
                     /locus_tag="ACHKNP_RS20250"
                     /old_locus_tag="pgaptmp_004050"
     CDS             complement(1856..2494)
                     /gene="leuE"
                     /locus_tag="ACHKNP_RS20250"
                     /old_locus_tag="pgaptmp_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HGU4706121.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(2621..3544)
                     /gene="dmlR"
                     /locus_tag="ACHKNP_RS20255"
                     /old_locus_tag="pgaptmp_004051"
     CDS             complement(2621..3544)
                     /gene="dmlR"
                     /locus_tag="ACHKNP_RS20255"
                     /old_locus_tag="pgaptmp_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HGU4706122.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            3647..4732
                     /gene="dmlA"
                     /locus_tag="ACHKNP_RS20260"
                     /old_locus_tag="pgaptmp_004052"
     CDS             3647..4732
                     /gene="dmlA"
                     /locus_tag="ACHKNP_RS20260"
                     /old_locus_tag="pgaptmp_004052"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HGU4706123.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            4983..6593
                     /gene="yeaV"
                     /locus_tag="ACHKNP_RS20265"
                     /old_locus_tag="pgaptmp_004053"
     CDS             4983..6593
                     /gene="yeaV"
                     /locus_tag="ACHKNP_RS20265"
                     /old_locus_tag="pgaptmp_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HGU4706124.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            6625..7749
                     /gene="yeaW"
                     /locus_tag="ACHKNP_RS20270"
                     /old_locus_tag="pgaptmp_004054"
     CDS             6625..7749
                     /gene="yeaW"
                     /locus_tag="ACHKNP_RS20270"
                     /old_locus_tag="pgaptmp_004054"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HGU4706125.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSIELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7805..8770
                     /gene="yeaX"
                     /locus_tag="ACHKNP_RS20275"
                     /old_locus_tag="pgaptmp_004055"
     CDS             7805..8770
                     /gene="yeaX"
                     /locus_tag="ACHKNP_RS20275"
                     /old_locus_tag="pgaptmp_004055"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HGU4706126.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8824..9939)
                     /gene="rnd"
                     /locus_tag="ACHKNP_RS20280"
                     /old_locus_tag="pgaptmp_004056"
     CDS             complement(8824..9939)
                     /gene="rnd"
                     /locus_tag="ACHKNP_RS20280"
                     /old_locus_tag="pgaptmp_004056"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HGU4706127.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPALISGWRGELMAEALHNLLQEYPQ"
     gene            complement(10021..11706)
                     /gene="fadD"
                     /locus_tag="ACHKNP_RS20285"
                     /old_locus_tag="pgaptmp_004057"
     CDS             complement(10021..11706)
                     /gene="fadD"
                     /locus_tag="ACHKNP_RS20285"
                     /old_locus_tag="pgaptmp_004057"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HGU4706128.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
BASE COUNT         2882 a         3051 c         2871 g         2948 t
ORIGIN      
        1 ttgctgaatt tgaccttgcg gcttaactgt cgctgaagtc tcgccagcgt cttctggttt
       61 ttctgaaaac tgtttacagg cccaaagact gtgccatctg acagcgtggc gagtttagcc
      121 acgccagcat ccagcccgat cattgatgct gaagggtgaa ccggagttga tacttcactt
      181 tctgtctgaa tactgatgta ccacttaccg caggactggc tgaccgtgac atttttcaca
      241 acacccgtga cctgacggct attccggtag cgcatccagc ccagtttcgg cagaaaaata
      301 cggctgtttt cctgatcgag ctttacgccc tgcgggtagc ggaatgcatc attctgtccc
      361 cgctttttga atcggggaaa agcagcccgc ttccggaaga agtttttgta agcccgctca
      421 aggtctttca gtgactgttg caatggctgt gagggcgaat ctttaagcca ttgcgtttca
      481 gtggcgtttt tccactcaac cagccaggaa gccattttcc cgtaagggat gtatttatta
      541 ccggcctcat gattctcatt ctgacgtgcc agagcacgat tgaaaacgaa acgacatgcg
      601 cctgcgaagc gcctcatttc acactcctgt tgaccacctg gtcttaactg gaatttaaat
      661 gcctgtaatc gtttcacagg tcaaatgata ctttggtctg tgaaaaaaga aaccgatatc
      721 cggcgtggaa ggcattgtgt tttcctgatg catgttcacc tggtctttgt caccagatac
      781 cgactccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt ttcaaatgta
      841 tgtgctgatt ttgaagctga acttgttgaa atggatggcg aaccagatca cgtccatttg
      901 ttaattaact atcctcccaa actggcgata tccagtctgg taaacagcct caaaggcgta
      961 tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta ctacaaaggc
     1021 gttttgtgga gtcctggcta ttttgccagt agctgcggag gtgcgccaat atccgtcatc
     1081 cgccaataca ttgaacaaca gcaaacacct ggttagatgg aaaaccgcgc cttatatccc
     1141 cgccctgaag gacggggttt tacggcgcac cggataagaa cgcacggtat tcaccagatc
     1201 ttttatcact tcagccgcca cttctggcac cagcaaagtc atcggcgtct ctgtttcata
     1261 atcgacagaa acgccattgc tgttattggt gacggtcacg gtatacgttg ctttgcccat
     1321 gattcatttc ccgttatgaa tgactttccg ttgttgcgca ccttccatca ggacttcagg
     1381 agccacgaag aagtcaatgt tgaaataagt atcgtcagtc atggcttcaa tgttgtgcca
     1441 cttttctgga gggaacaccg caaactgccc cgcttcgata aggatcacct gatcaggctc
     1501 tgcactgtgt tcatcagcgt agccgagata tttgaccgcc ccatgcataa cggaaaggcg
     1561 tgggtaaacc cccgggcgcg ttcctttatc aagatgacgt tcgaatattc cggcaggcgc
     1621 agtttgttta ttccagaaag gcgttgagcg cgtatgaata taattctgtg ggatttgcag
     1681 catcctattc cctccttcgg ggaatgcgct gaaaacggtt tattccagcc gtttcagggt
     1741 acgcctgata atttgcattt taaataccat ttattggtta ctttttagca ccatatcaga
     1801 gaaaaatcag ggaggattac aagtggggag cccgcgacaa cgcgggctga atgtatcagg
     1861 attgcagcgt cgccagtcgg gcggcgaaac ccacgaacat caaaccaatc agtgagttgc
     1921 caactttagc cagtttcttt ttggtacgta tgtactgcgt gacaaaagca ccagaaataa
     1981 tcaggaagct caaatagcag aaactcacca gttccagcgt cgtcgccaga ataaagaatg
     2041 aaattcccgt atgtggggca ttaacatcga taaactgtac gaaaaacgac acatagaaca
     2101 aaatggcttt cggattagtc aggctcaaaa ttaacgcgcg tttaaaaatg gccccgtatt
     2161 ggggctcatc ggatttggcc tcgttatttt tacccttcag ggtcgcgtag agaattttac
     2221 tccccagata gagcaaataa aacgcaccaa gataacgtac aatgttgaat aatatcgggg
     2281 tggtcttaat taatgtcgcc actccagccc atgccagaaa catcaatact gcatcgccaa
     2341 taaatacacc gcaggccgca agataaccgc ctttcatacc gctactgacg ctatttttga
     2401 gtacaaacag ggtatttggc cctggcacca acacaataaa aatggcccca accagatagg
     2461 tccagtaatt cagaaccccg tattcagcga acacattaac ctctttaatt atctttcgat
     2521 catgcgcgat taaaggtgaa tatgctaacc aatctgtagc ggcttagaaa ggagaaaatc
     2581 aggttttaac ccgataccaa cccgataatt gaatcattaa tcaggcatgc tccagtgaaa
     2641 aattcgggta gtgctcggca aaatactggc gtaaaaattc taccgttatc cgcactttcg
     2701 ctgacgtcgc cagccttgaa acataaacgg cccagacgtt cgctggctgg taatattccg
     2761 gtagcacttg cactaaatga ccactggcaa tgttttcgct aacatcccac caggagcgca
     2821 gagcaatccc ctgcccatcg agacaccact gatggacaat ttctccatga ttagacgaca
     2881 gtgggccggt gactttaatc gcatggtggc cttctttatt gcgtaattgc cagacaccaa
     2941 aaggatgatc gcgctcttta atgaccaggc acggcaatgc ggaaagatcg gttaaatgtt
     3001 tcggcgcgcc atgttgcgca ataaattccg gcgacgcgca gagaatgcgg taattagtcg
     3061 ccagtttgcg ggcgattaaa ttaggggcga tgtcatcgcc aatgcgaata tcgagatcaa
     3121 caccttcatt gaccaaatcg accaaccggt cttccacatc aaaacgtaat tccagttgcg
     3181 gatacgcctt agccagcgct gataacgccg gagccaccac ctgtcgtcca aaaccaaagc
     3241 tgctgataat acgcagcatc ccctgcggca cctgacgcac gtcagaaagt tcgtccatca
     3301 tctgaccgac atcctgcaaa atccgctgcg cccattcata aatccgctct ccttcttcgg
     3361 taatggtgac gcggcgggtg gtgcggtgta acaacaccac gtttagcgtt tgctccagca
     3421 aggcgatgcg cttgctgacg aacgccggtg aaacgcccag ttcttccgcc acggcggcaa
     3481 aaccagcccg acgagccacc agcataaaga cgcgcaaatc attcagcagc ggtaaattat
     3541 tcatgattcg tgttttatgt ttcaccagtt acggggatta attccttttc agtcaattat
     3601 aggatggtga tgttgtcaat tttgatggtc aggaagtgag aacccaatga tgaaaacgat
     3661 gcgtattgct gcgatcccgg gagacgggat tggcaaagaa gtccttcctg aagggattcg
     3721 cgtgttacag gctgccgctg agcgctgggg cttcgccttg agttttgagc aaatggagtg
     3781 ggcgagctgc gagtattaca gccatcacgg taaaatgatg ccggacgact ggcatgagca
     3841 acttagccgt ttcgacgcca tctattttgg tgccgtcggc tggccggaca ccgttcctga
     3901 ccatatttcg ttgtggggtt cgctgctgaa atttcgtcgt gaattcgacc agtacgtcaa
     3961 cctgcgcccg gttcgtctct ttcctggcgt tccctgcccg ctggcgggaa aacagcctgg
     4021 cgacatcgat ttttacgtgg tcagggaaaa caccgaaggc gaatattcct cgctcggcgg
     4081 tagagtgaat gaaggtacag agcatgaagt cgtcattcag gaatcggtat ttacgcgtcg
     4141 tggtgtcgat cgcattttgc gttatgcctt cgaacttgcg caaagccgcc cacgtaagac
     4201 gctaacttct gccactaaat caaacggttt agccatcagc atgccgtact gggatgagcg
     4261 agtggaagca atggccgaga attacccgga gatccgctgg gacaagcagc atattgatat
     4321 tctctgcgcg cgttttgtga tgcagccgga acgcttcgat gtggtggtgg cgtccaattt
     4381 atttggcgat atcctttccg atcttggccc ggcctgcacc ggcaccattg gcattgcccc
     4441 atccgccaac ctgaatccgg aacgcacttt cccgtcgctc ttcgagccag tccacggttc
     4501 cgcgccggat atctacggga aaaacattgc taaccctatc gccacgattt gggccggtgc
     4561 aatgatgctc gattttctcg gcaatggcga tgagcgtttc cagcaagcgc ataacggtat
     4621 tctggcagca attgaagaag tgattgctca cgggccgaaa acacctgata tgaaaggcaa
     4681 tgccaccacg ccacaggttg ccgacgcgat ttgcaaaatt attttgcgtt aaggtcaaac
     4741 cagtttattt gaaccgcgtc actgacgcgg tttttttatt cgttctttgc agtaaataac
     4801 ctgcgtcatt tcacctttta ttgtttccgt ttcgtgtttt atggctttcc gtattcttaa
     4861 ttgtttaatt tatgtaacat gcaaataatc aatttgttaa acacaacata caactcatta
     4921 ccctgcaaat aattacaact ggcaaaagcc agagacgggc ttttatgccc aggagatgac
     4981 tcatgatgag caatgttaag aaaaaagatg tgccgctgat aagcatcagc ctggtggcca
     5041 ttcttttcat cgcagctgca ttaagccttt tcccacaaca atcggccgac gcggccaacg
     5101 caatatacac ttttgttacg cgtacgttag gttccgccgt acaggtattg gttttgctgg
     5161 caatgggact ggtgatttat ttagccacca gtaaatacgg caatattcgt cttggcgaag
     5221 gaaaaccgga atacagcacg ctctcctggc tgtttatgtt tatttgtgcc ggtttaggtt
     5281 cttctacgct ttattggggg gttgctgaat gggcctatta ttatcaaacg cctggattaa
     5341 atatcgcacc gcgttcacaa caggcactcg aatttagcgt cccttactcc ttcttccact
     5401 ggggcatcag cgcctgggca acttatacgc tggcctcatt aatcatggct tatcactttc
     5461 atgtgcgtaa aaacaaaggt ctgagtcttt ccggcattat tgccgcgatt accggcgttc
     5521 gcccgcaagg cccatgggga aaactggttg atttgatgtt cctgatcgcc actgtcggcg
     5581 cactgactat ttcccttgtt gtcaccgcag caacctttac tcgtggactt tccgcgctga
     5641 ccggtttacc cgataacttc accgtgcagg catttgtgat cctgctttcc ggcggcattt
     5701 tttgcctaag ctcgtggatt ggtatcaaca acggtttgca acgtctgagc aaaatggttg
     5761 gctggggcgc gttcctgctg ccattactgg tgctgattgt cggcccaacc gaatttatta
     5821 ccaacagcat catcaatgcc atcggtctga ccacgcaaaa cttcctgcaa atgagcttat
     5881 tcaccgatcc gcttggcgat ggttcattta cccgcaactg gaccgttttc tactggctgt
     5941 ggtggatctc atacacccct ggcgtagcaa tgtttgtcac ccgcgtttcc cgcggtcgta
     6001 agattaaaga agttatctgg ggactgatcc tcggcagcac cgtcggttgc tggttcttct
     6061 tcggcgtaat ggaaagctat gccattcatc agtttatcaa tggcgtaatc aacgtcccac
     6121 aggtgctgga aacactgggc ggcgaaacag ctgtacagca agttctgatg tcgttgccag
     6181 ccggtaaatt gttcctcgcc gcatacctgg gcgtgatgat tattttcctt gcctcgcata
     6241 tggatgcggt ggcctacacc atggctgcga ccagtacgcg taatctccag gaaggtgacg
     6301 atcctgaccg tgggctgcgt cttttctggt gcgtggtgat cactctgatc ccgctttcca
     6361 tcttgtttac cggtgcttcg ctggaaacga tgaaaaccac cgtcgtgctc acagcccttc
     6421 ccttcctcgt cattttactg gtgaaagtcg gcggatttat tcgctggctg aaacaggatt
     6481 acgccgacat tccggctcat caagttgaac attatctccc gcagacaccg gttgaagccc
     6541 tggaaaaaac gccagtgctc cctgcgggaa ccgtattcaa aggcgacaac tgagcgccat
     6601 catcctaacg ataaaggtat ccctatgagc aatctgagcc ctgactttgt actacccgaa
     6661 aatttttgcg ctaacccgca agaggcgtgg accattcctg cccgttttta taccgatcag
     6721 aacgcgtttg aacacgaaaa agagaacgtc ttcgccaaaa gctggatttg cgtcgctcac
     6781 agcattgaac tggcgaatgc caatgattat gtgacgcgtg agatcattgg cgaaagcatc
     6841 gtgctggtac gcggtcgtga taaggttttg cgcgcgttct ataacgtgtg tccgcaccgt
     6901 ggtcatcagt tgttgagcgg tgaaggaaaa gcaaaaaatg tgattacctg cccgtatcac
     6961 gcatgggcat tcaaactcga tggcaatctg gcccatgcac gtaactgcga aaacgtcgcc
     7021 aatttcgata gcgacaaagc gcaactggtt ccggtgcgtc tggaagaata tgccggattc
     7081 gtcttcatca acatggaccc caacgccacc agcgtagaag atcagttacc cggtctgggc
     7141 gcgaaagtgc tggaagcctg cccggaagtc cacgatctga aactggcggc ccgctttacc
     7201 acccgcacgc ctgccaactg gaagaacatt gtcgataact atctcgagtg ctatcactgt
     7261 ggtccggcgc atccaggttt ctccgactcc gtacaggttg atcgttactg gcacaccatg
     7321 cacggtaact ggacgctgca atacggtttc gccaaaccgt ccgaacagtc gtttaaattt
     7381 gaagagggta cggatgcggc attccacggt ttctggctgt ggccgtgcac gatgctgaac
     7441 gtcaccccga tcaaagggat gatgacggtc atttatgaat tcccggtgga ttctgaaact
     7501 accctgcaaa actacgatat ttacttcacc aatgaagagt taaccgacga gcaaaaatcg
     7561 ctgattgagt ggtatcgcga tgtgttccgt ccggaagatt tacgtctggt tgaaagcgta
     7621 cagaaagggc tgaaatcgcg tggctatcgt ggtcaggggc gcatcatggc cgacagtagc
     7681 ggtagtggca tttccgaaca tggtatcgcc catttccata atctgctggc gcaggtgttt
     7741 aaggactaat gacatcggcg gcggtatttt ccgccgctgg gctgattttt gatggagtac
     7801 agcaatgtca gactatcaaa tgtttgaagt acaggtgagt caggtggaac cgcttaccga
     7861 acaggtgaaa cgcttcacgc tggtggcaac cgatggcaaa ccgctgcctg cgttcaccgg
     7921 aggaagtcac atcattgtgc agatgagcga tggtgataac cagtacagca atgcgtattc
     7981 actactgagt tcgccgcatg acacctcttg ttatcagatt gccgttcggc tggaggaaaa
     8041 ctcgcgcggc ggttcccgct ttttgcatca gcaggtaaaa gtgggcgatc ggttaacgat
     8101 ttcaacgcct aataacctgt ttgcgctaat tccctcagcc agaaagcatc tgtttatcgc
     8161 gggcggtatt ggtatcaccc ctttcctgtc gcacatggca gagctgcaac acagcgacgt
     8221 cgactggcag ctacattact gctcgcgaaa tccagaaagt tgcgcatttc gtgatgagct
     8281 agtccagcat ccgcaggctg agaaagttca cttgcatcac tcatcaaccg gaacacgact
     8341 ggaattagcg cgattattgg cggatatcga acctggcaca cacgtttata cctgtggtcc
     8401 cgaggcgcta aatgaagcgg taagaagtga agctgcgcgt ctggacatcg tcgccgatac
     8461 gctgcacttt gagcaatttg ctatcgaaga caaaaccggc gatgccttta ccctggtgct
     8521 tgcccgttcc ggaaaagagt ttgtggtgcc ggaagagatg actattttgc aggttattga
     8581 aaataataaa gccgcgaaag tggaatgttt atgtcgtgaa ggggtatgcg gaacctgcga
     8641 aacagcaata ctggaaggtg aagctgacca tcgggatcag tattttagcg atgaagagcg
     8701 tgccagccag caaagtatgt tgatctgttg ttcgcgtgcg aagggtaaac gcctggtgtt
     8761 ggatttgtag tttgccaaag ccggatgtgg cgctgagcgc gcccagtccg gcttcggaag
     8821 attttactgc ggatattcct gcaataaatt gtgtaatgct tccgccatca gctcaccacg
     8881 ccagccggaa atcagcgccg gcaaattgtt ctgcggtttc agtttccagt gccagttcag
     8941 cagttggttg atttgccgac gcgatgccag caattcggcg ctgatcttat gcgtttcgct
     9001 cacgtcagta atcagcgact taatcgcttt aaacacttta cgataaccag gcatgtccat
     9061 caggttaagc atcggctgcg gtaaggcctc ttccggcaat gtctgcgctt tttccaccag
     9121 cgccagcagc gttttaccgt gaaagcggat ttcgctaccg gataaaccca ggctgtccag
     9181 ttcgcctaaa cttcccggca tataacgcgc gaccgaccac aaatgctctt cacgcacgac
     9241 aaagttcacc gccagatcac gctctcgcgc cttgcgcagt cgccagtcgg ctaacagttg
     9301 cagacaggcc agttggcgtg tgcgtaactg ccaggcattg gtgatatcac gccaggcatc
     9361 ttccggcgca acgacttcct gacgacgcat ttgcatcagg cggcattcat ccagcgccgc
     9421 aggtagccag ccggaggcct ccgtttctac cataagcttg gcggtgatcg gtaacagata
     9481 ccagacatcc gccgctgcgt attcacactg acgttcggtc agcggtctgg ccagccagtc
     9541 ggtgcgcgat tcactcttgt ccagcgtaac gccggaatac tcttccacca tggaagcgaa
     9601 accccatgac atcgggcgtc cgcagaaggc agcaaggatt tgcgtgtcaa tcaagggttg
     9661 tggtaattcg ccaaagacat tgaggaacac ttccagatct tcactgcctg catggagaaa
     9721 ttttgtgatg gacggatcgc gcaggatcgc tttcagcggt gaccagtcgg tgatcccgag
     9781 aggatcaatt agcgccagat gctcgccatc gaaaagttga atcaacccca gctgcgggta
     9841 ataagtgcgc gtacgaacaa attcagtatc cagggctatc gccggaaagg cacggacggc
     9901 ttcacacaaa gaagccagcg catcgtccgt ggtaatcatt tggtaattca aattgttttc
     9961 tctttagtgg gcgtcaaaaa aaacgccgga ttaaccggcg tctgacgact gacttaacac
    10021 tcaggcttta ttgtccactt tgccgcgcgc ttcgtcacgt aattctcgtc gcaaaatttt
    10081 tccgacgtta gatttcggta actcatcacg aaactccacc agcttcggta ctttgtagcc
    10141 cgtgagctga cggcggcaaa aggtcaccag tgactcttcg gtaagcgatg gatctttttt
    10201 cactacgaag attttcaccg cttcaccact ggagccggaa ggtacgccaa cagccgcgac
    10261 ttcctgtacg ccaggatgct gcatgacgac atcttcaatc tcgttgggat agacgttaaa
    10321 accggaaacc agaatcatgt cttttttacg atcgacaatg cgcaggaacc cttcttcatc
    10381 catcaccgcg atgtcgccgg tgtgtaacca gccatttttg atgatctcat ctgtagcatc
    10441 cggacgctgc cagtaaccca gcatcacctg cggtcctttg acacaaagct cacccggttg
    10501 acccggtggt acttcattat catcatcatc caccagtttg gcttccgtcg acggcaccgg
    10561 caaaccgatg ctaccactat gataatcaat atcatatggg ttaacgctga ccagcggcgc
    10621 acactcggta aggccatagc cttccagcag atactgtcct gtcagtttca cccaacgctc
    10681 tgccaccact tgctgcactg gcatccctcc gcctgcggaa agatgcagac tggagaaatc
    10741 cagctgctgg aactctttat tgttcagcaa cgcattgaac aaggtgttaa cgcccgtgat
    10801 agcggtaaac ggatatttcg ctaactcttt taccaaccct ggaatatcgc gcgggttagt
    10861 gataagcagg ttctgcccac ccagttcgat aaacagcagg cagttaatgg tcagggcaaa
    10921 aatgtgatac agcggcagcg ccgtcaccac cagctctttg cccggatgca acagcggacc
    10981 ataggtcgcg ttaacctgtt ccaggttcgc cagcatattg cggtgagtca gcatcgcgcc
    11041 tttcgccaca ccagtggtgc cgccggtgta ttgcagaaaa gctaaatctt ccggcaccag
    11101 ttcgggtttg acgtactgca tccggtagcc gttatgcagt gcgctacgaa atgaaatggc
    11161 atctggcaga tggtatttcg gcaccaaacg cttgatgtat ttaacaacga aattgactac
    11221 cgtgcctttt gccgtagata gctgatcgcc catacgggtc agaattacgt gctgaacggc
    11281 ggttttatca accacttttt ccagtgtgtg agcaaagtta gacacgataa caatcgccga
    11341 tgcgccgcta tcgttaagct gatgctcaag ctcacgcggg gtatacaacg ggttaacgtt
    11401 tacgacgatc atcccggcac gcaaaatgcc aaacagcgcc accggatatt gcaataaatt
    11461 aggcatcatc aacgcaacgc gatcgccttt cttcagcccc aacccttgtt gcaaataagc
    11521 ggcaaacgcg cgactgcgtt cttccagctt gcggaaggtc attacctccc ccatattcac
    11581 aaacgcaggt tgatcggcgt agcgcgcgac cgactgctca aacatatcta ccagagattg
    11641 ataacggtca gggttgatct ccgtcggaac gtccgcggga taacggttaa gccaaacctt
    11701 cttcaattct tcacctctaa aatgcgtgtt cgtcgtcatc gcaaccccaa at
//
LOCUS       DAZFWL020000141        11709 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFWL020000141 DAZFWL020000000
VERSION     DAZFWL020000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.141"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            279..1577
                     /gene="kgtP"
                     /locus_tag="ACHKNP_RS20290"
                     /old_locus_tag="pgaptmp_004058"
     CDS             279..1577
                     /gene="kgtP"
                     /locus_tag="ACHKNP_RS20290"
                     /old_locus_tag="pgaptmp_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HGU4706129.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1574..1897)
                     /gene="yfiM"
                     /locus_tag="ACHKNP_RS20295"
                     /old_locus_tag="pgaptmp_004059"
     CDS             complement(1574..1897)
                     /gene="yfiM"
                     /locus_tag="ACHKNP_RS20295"
                     /old_locus_tag="pgaptmp_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HGU4706130.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1943..3298)
                     /gene="pssA"
                     /locus_tag="ACHKNP_RS20300"
                     /old_locus_tag="pgaptmp_004060"
     CDS             complement(1943..3298)
                     /gene="pssA"
                     /locus_tag="ACHKNP_RS20300"
                     /old_locus_tag="pgaptmp_004060"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HGU4706131.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3412..6072)
                     /gene="pat"
                     /locus_tag="ACHKNP_RS20305"
                     /old_locus_tag="pgaptmp_004061"
     CDS             complement(3412..6072)
                     /gene="pat"
                     /locus_tag="ACHKNP_RS20305"
                     /old_locus_tag="pgaptmp_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HGU4706132.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6104..6802)
                     /gene="tapT"
                     /locus_tag="ACHKNP_RS20310"
                     /old_locus_tag="pgaptmp_004062"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6104..6802)
                     /gene="tapT"
                     /locus_tag="ACHKNP_RS20310"
                     /old_locus_tag="pgaptmp_004062"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HGU4706133.1"
                     /translation="MTENGVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6871..7290)
                     /gene="trxC"
                     /locus_tag="ACHKNP_RS20315"
                     /old_locus_tag="pgaptmp_004063"
     CDS             complement(6871..7290)
                     /gene="trxC"
                     /locus_tag="ACHKNP_RS20315"
                     /old_locus_tag="pgaptmp_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HGU4706134.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7497..8534
                     /gene="yfiF"
                     /locus_tag="ACHKNP_RS20320"
                     /old_locus_tag="pgaptmp_004064"
     CDS             7497..8534
                     /gene="yfiF"
                     /locus_tag="ACHKNP_RS20320"
                     /old_locus_tag="pgaptmp_004064"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU4706135.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8582..9271)
                     /gene="ung"
                     /locus_tag="ACHKNP_RS20325"
                     /old_locus_tag="pgaptmp_004065"
     CDS             complement(8582..9271)
                     /gene="ung"
                     /locus_tag="ACHKNP_RS20325"
                     /old_locus_tag="pgaptmp_004065"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGU4706136.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9576..9959
                     /gene="grcA"
                     /locus_tag="ACHKNP_RS20330"
                     /old_locus_tag="pgaptmp_004066"
     CDS             9576..9959
                     /gene="grcA"
                     /locus_tag="ACHKNP_RS20330"
                     /old_locus_tag="pgaptmp_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HGU4706137.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10015..10602)
                     /gene="eamB"
                     /locus_tag="ACHKNP_RS20335"
                     /old_locus_tag="pgaptmp_004067"
     CDS             complement(10015..10602)
                     /gene="eamB"
                     /locus_tag="ACHKNP_RS20335"
                     /old_locus_tag="pgaptmp_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HGU4706138.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10705..11586
                     /gene="yfiE"
                     /locus_tag="ACHKNP_RS20340"
                     /old_locus_tag="pgaptmp_004068"
     CDS             10705..11586
                     /gene="yfiE"
                     /locus_tag="ACHKNP_RS20340"
                     /old_locus_tag="pgaptmp_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4706139.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNASSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         2915 a         2924 c         3027 g         2843 t
ORIGIN      
        1 ttggtgaaaa aaatgcctga tgcgacgctg cgcgtcttat actcccacat atgccagatt
       61 cagcagcgaa tacggcttcc ccaacttgcc cacctccata cgtgttctcc tcaccagaaa
      121 tttatcctta agctcctcaa taaccatttt cctgctaact aaattcatgg ttaaggttgc
      181 ataatgatac gcaacaaatg tataacattt tctttaccaa aagaaataaa caaaagcgac
      241 cgacaaaagc atcggattac ggcaggagac ataatggcat ggctgaaagt actgtaacgg
      301 cagacagcaa actgacaagt agtgatacac gtcgccgcat ttgggcgatt gtgggggcct
      361 cttcaggtaa tctggtcgag tggttcgatt tctatgtcta ctcgttctgt tcactctact
      421 tcgcccacat tttcttccct tccgggaaca cgacgactca actactacaa acagcaggtg
      481 tttttgctgc gggattcctg atgcgcccaa taggcggttg gctatttggc cgcatagccg
      541 ataaacatgg tcgcaaaaaa tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc
      601 tggttatcgc ctgcctccca ggttatgaaa ctataggtac gtgggctccg gcattattgc
      661 ttctcgctcg tttatttcag ggattatccg ttggcggaga atatggcacc agcgccacct
      721 atatgagtga agttgccgtt gaagggcgca aaggttttta cgcatcattt cagtatgtga
      781 cgttgatcgg cggacaactg ctagccctac tggttgtcgt ggttttacaa cacaccatgg
      841 aagacgctgc actcagagag tggggatggc gtattccttt cgcgttagga gctgtgttag
      901 ctgttgtggc gttgtggtta cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg
      961 ctttaaaaga agctggatct ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg
     1021 ttctcggttt taccgctgcg ggctcccttt gtttctatac cttcaccact tatatgcaga
     1081 agtatctggt aaatactgcg ggaatgcatg ccaacgtggc gagtggcatt atgactgccg
     1141 cattgtttgt attcatgctt attcaaccac tcattggcgc gctgtcggat aagattggtc
     1201 gccgtacctc aatgttatgt ttcggttcgc tggcagccat ttttaccgtt cctattctct
     1261 cagcattgca aaacgtttcc tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc
     1321 tgatagtgag tttttataca tcaatcagtg gaatactgaa ggctgagatg ttcccggcac
     1381 aggttcgcgc attaggcgtt ggcctgtcat atgcggtcgc taatgctata tttggtggtt
     1441 cggcggagta cgtagcgttg tcgctgaaat caataggaat ggaaacagcc ttcttctggt
     1501 atgtgacctt gatggccgtg gtggcgtttc tggtttcttt gatgctacat cgcaaaggga
     1561 aggggatgcg tctttagtga cgggtcagtt gccagacggt atagccggtg cttgcaccgg
     1621 cgacatccca ggccaaatcc ttccagctcc agccgctccc ttcggggcgg ctatcccaaa
     1681 gctcttttga cgcccccaaa ctgacagaga acatcaatcc aaacatggca ctgcgatccc
     1741 ggctcatccc ctgatgctgt gaatattcat ttccggcggc ggaaagcatc gccgaggcga
     1801 taaagtgttg agctttatcc tgcccgctcc agctatcgtt agccatatgg ctgcatccag
     1861 aaagtaacaa tagcgaacag acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac
     1921 cccgtacagg acggggttgt gattacagga tgcggctaat taatcggtcg atgcggatac
     1981 ggcgcaaacg gcggatgagt ttacgaacct tcaccggata atcggcaata ctttgcagat
     2041 cgcgatagtg cttaacgatg gtggtatgct cgcggatcag ctccagttct ttctctcgct
     2101 gcggcgcaag ctccagttgt ggatcgtgga tcaaaatggc gttttccaga tccagacgcc
     2161 aggcgcgcgg gttcaggtta ttaccggtga tcaacatcca cttatcatca acccacatcc
     2221 cttttaggtg ataggtgttg tcgtcatctt tccataaccg aaccactagc tggtcagtat
     2281 tgacgtaata ctgcaaacgg ctcaggaaac gacgcagatt gatctcatag agataaggca
     2341 atgcgccaat tatcttgaaa ggttcatctt ccggaatgta gaagtcgttc gcggttttat
     2401 caccaacaat aatttcgacc tttttccctt cgcgcagcaa ctggataata ttgcgcacaa
     2461 ggattgctgg caggttgaag tatggcgtac agatggttag tttctgctcg gcacaaggca
     2521 taagatggaa aatggtcttg ttcaacagac tcgatttccc cagccccact agcggcgtta
     2581 cggaaagctg atcgttgtcg gcatcgccct ggaaatgata agcggcatca cgcagctcct
     2641 ggcggaacag acgaatatcg ttcttgattt ccgggctttt tggccgatta acatcatcca
     2701 gacgattaac gccgcggcca ttcataatat tctgtgtaac ccattcaaac ataatgtctg
     2761 acatcttacg gttacggata agatgataac ggtcgtagcg atatttatcg tgctgatgca
     2821 ggtaaacatc gttcaggctg gcaccgctat aaagtacgct atcgtcgatg ataaagcctt
     2881 taaagtgcag aacaccaagg gcttcacggg tattgattgg aacgccataa accggaacat
     2941 ctacgcccgg attttcctgc gccatgcggc agtaccagtc agcgttagtg ttagatgccg
     3001 cagcgccaat gcgtccacgt tgtgcacgat gccagtcgac cagcacccgc acatccagtt
     3061 ccggacgctg ccttttagct tcatacaacg cgttcagaat gcctttgcca ccgtcatcct
     3121 gttcgagata cagggcgaca atgcaaatgc gctgcttcgc gctggctatt ttttccagca
     3181 gcgtctcccg gaagtcggcg ggagcgtaaa agaaatcgac atcatcaact gattgagaaa
     3241 tcttgggtag ttgggcaagg tgttgttgat gtttattacg cttaaatttt gacaacatca
     3301 cagtgcattt cttctctgtt cattgaaggg tcctctgtgc aatgcagacg acataagcgg
     3361 gcaataataa caccagtccc gattaagtgg tcaacatttc cagtacctta ctcatgattc
     3421 ctcgcgttgg gcaagattta gcgtaagccc aacgatcccc tcttcgagct ggatatcaac
     3481 gttaaacccg agcttgcggg ctagcgccac catgccacga ttgtttggca tcgtaatacc
     3541 attcagacgt tgtagtccgt gatctcgcgt ataggtaatc aacttttcca ttaagcgtcg
     3601 acctaagcct aaccctttga gatccgagcg aaccagcaca gcaaattcgg catcgatgtt
     3661 atcaggatcg gaaatcgcac gcgtgacgcc gaggatctct tccgtttgat caatacgtcg
     3721 taccgctaca aacgccattt cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc
     3781 atgggtaaat tcgttgatct cgctaaagta gcggtaataa agatcttctt tggtgactcg
     3841 cgaaatgaat tgctgaagtt gtggctcatc ttctggcaaa atcgggcgga acaagcagcg
     3901 ttcaccgttt ttcaattcta cccattcttc cagctgatgc ggataagggc gcactgccag
     3961 ccgactctcg ttatcgcctt caaacggcga gatatccagc gtgacatcca gcgcggtaaa
     4021 ttcactgcca gaagccagca aaggatgaat atccagacgc tgaatttccg ggcaatcgac
     4081 aatcaagttg gaaacctgca ccagaagctg gctcaagcct gcaacatcca atgggcgtag
     4141 cgcactgcgt gcacgaatct ttttactttt gatcccctga ataaccagat agcgggccag
     4201 gttcatgttc agcggcggta acgcgacgac ggcttgatct tcaggacgcc actccacacc
     4261 gccttcgccc agcatgatca acggcccgaa aaccggatcg tgctcaacca caacccgcaa
     4321 ctcctgagca ccagcacggt tagccatact ttgcaccaac aggccgtgga cccgtgcctg
     4381 cggccaggcc atttttacgc gatcgaaaat ggcgtttgcc gcttgctgga cttcattggc
     4441 tgtacgcagg tagagcatga cgccctgaac ttccgattta tgtggaatat ccggcgaacg
     4501 caatttcagc gccaccggat aaccaatctg ttcggcaata tgcaccgctt cggtgctatc
     4561 gctggcaatc caggtgggga gcgtgttcat gccatatgct tgcaggatag gctgaacttc
     4621 atgggtatcg agtgacgttg ctccttcggc aatcgcctgt tgcaacagaa gatgcgcttc
     4681 tgcggtattg gaggtcagat tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt
     4741 acgccggtac tccaccatat gcataaaagc agtgatggtt ccttccgggg tacggtaggt
     4801 cggcagcccg gcttcgctga ataatcgtcg tgcctcttgc gaggaatgtt cgccacacca
     4861 gttcgtcagc agagaaacat acttgctgcg gggatgatgc tttaccgctt caattaatac
     4921 ttgcgcgctt tctgttgcgg gagcagcggc gctgggcgaa tgaataacca tcagcgcatc
     4981 aaaatcctgg ctgtggagca gaatgtccag cgttttaata tagtgctcac tgctggcgtc
     5041 atcgcgcaga tcgagcgggt tagagattgc cacatgtcct ggcagtgcat cgcgcagttt
     5101 ctggcaggtt tcttcgctta gtgttgccag cttgccattg cgtgaccata aggcatcaag
     5161 cgccagcgcg gcaggcgcag caccgttgct gataatcatc aaccggtcgc cacgcagcgg
     5221 gcgcatatgg ctaagggttt ccaccgccga aaacagttcg tgggtgtcct gtacccgcaa
     5281 caaaccggca cgctgaatag ccgcatccca cgccggatcc attcctgccg tggtgttcaa
     5341 cagacgttga gccgccgggc tacgtccgct tttaatcacc agaatcggtt tgttgcgaga
     5401 ggcactacgg gccgccgaaa caaagcgtcg cgcgtcgctt aactgttcga gatagagcag
     5461 gatggcgctg gttttactgt cgcgtgccag atagtcaagc aattcatcaa cgtcgatatc
     5521 caggctgtcg ccgagcgcaa taaagtagga aaagcccatc ttacgctgtt gcgcccagtc
     5581 gaggatggtg ttggagacgg cagccgattg cgaaataaac gccagcttgc cgcgtttaat
     5641 cggcacaggc gaaaagctgg cattcagacc ttgccaggga gccagtaaac ccagactgtt
     5701 tggtccaagc aggcgcatgt tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga
     5761 tgccggggcg gaaagaataa tgcaggtttt acagcctttc tcgccgagct cttccagaag
     5821 agcaagatta cggctggcat tggtacataa aaccgcaagg tcgggtgtaa agggcaagct
     5881 ggcaatatcc ggccaggcca acacacccaa caccgctttc caggctggcg tcaccgggag
     5941 taccggtccg ttaaagcctc ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt
     6001 gggtttcatc gacgcgccaa ttaccgctat cgattttggt cgcagtagtg cttccagtcc
     6061 tcgctgactc ataccggtct cccgcagaag tgaccgaatg attttaaacg ctttctaact
     6121 gttctgctgt gatgctaccc agatgttgcg tttttcctgc cagatagcgt gttttaaagc
     6181 gggtaaaatg ctcgcctaac cctgctgccg ccccggtatc gctggccata tctaacagtg
     6241 cgatggctac ctcggcagta caatattggc ctgcagcctg ggcttcacgc aggcgatagg
     6301 cagaaagccg ggaaagatcg acggaaatga cgggaagatt atccagatac ggacttttac
     6361 gaaacatctt gcgagcttcc ggccaggtac catcgagcat gataaacagc ggtggcttac
     6421 cggcaggtgg tgtgaagatc acttcccgtt gctcatcagc atacgaagcg ggaaagacca
     6481 ccattggctg atagtccggg ttttgcacca actccagcaa atcctgcgag ggttcggtac
     6541 gcgaccattg aaacgcaacg gtatcaggca aaatatcagc aatcagacgc ccggtattac
     6601 tgggcttcat tggctcggtg tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg
     6661 gggtaattgt cgaacagaga cataatttct ctggcaaaag acagcgttgg cagcgacgaa
     6721 cgcgattacc gcgggcaaga aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact
     6781 ggagaacacc gttttcggtc ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga
     6841 aaacggggca caagatgcgc cccggtaaga ttaaagagat tcgttcagcc agctatcgaa
     6901 cggcgctttc ggtactgcgc cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat
     6961 gatcgtcggt atactacgaa ttccaaagcg actgctcaat tcacgttcag cttcggtatt
     7021 cactttcaca aagcgcactt taccgctacg ctcttgcgcg acatcttcaa aaattggtgc
     7081 gaaattacgg caggggccgc accacggtgc ccagaagtcg atcaccacag gtagatcatc
     7141 cttcagcaat ttgtcgagtg tttcaccggt cgcattaatc acctctccgt caaacaagtc
     7201 gtgaccgcag cgtccgcatt ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat
     7261 ggcctgacaa tgggtacaaa cggtattcat aactaacctc gggatgagta aggagactta
     7321 tcggcaacga cgccggtatg tttctaatat gttacatatt atcggggaca ttgttccaga
     7381 caacaatctg ttttattcaa taggtatgat agtcgcaggc tttggtacga aatcatggcg
     7441 aaccgcgcgg acttcgggta atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga
     7501 acgatgaaat gaaaggtaaa agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg
     7561 attctgataa acgtacccac aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat
     7621 ctcgtgctga cggtggccgt cgccccgccc gcgatgacaa acagagtcag ccccgtgacc
     7681 gcaagtggga agattcgccg tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg
     7741 aaaaggccga tcacggtggc atcagtggta aaagttttat tgatccggaa gtgttgcgtc
     7801 gtcagcgtgc ggaagaaacc cgcgtctacg gcgaaaatgc atgtcaggca cttttccaga
     7861 gccgtccgga agcgattgtt cgcgcctggt ttatccagag tgtaacgccg cgttttaaag
     7921 aagccttgcg ctggatggca gcaaaccgca aagcgtacca tgtggtggat gaagcggaat
     7981 tgacaaaagc gtcaggcacg gaacatcacg gcggcgtttg cttcttgatc aaaaagcgta
     8041 acggtacaac cgtgcagcag tgggtaagcc aggcaggcgc gcaggattgc gttctggcac
     8101 tggaaaacga atctaacccg cataacctgg gtggcatgat gcgcagctgc gcgcacttcg
     8161 gcgtgaaagg tgtcgtggtg caggatgcgg cactgctgga gtcgggggcg gctatccgta
     8221 ccgcagaagg cggcgcagag cacgttcagc cgattactgg cgacaacatt gttaacgtgc
     8281 tggatgattt ccgtcaggca ggttacaccg tagtgaccac ttccagcgag cagggtaaac
     8341 cgctgttcaa aactagtctg ccagcgaaaa tggtactggt gctgggtcag gaatatgaag
     8401 ggttaccgga tgccgcacgc gatccgaacg atctgcgcgt gaagattgac ggtacgggca
     8461 acgttgccgg gctgaatatc tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc
     8521 agaataaagc ctgataaatc agccgggtgg caactctgcc atccggcatt tccccgcaaa
     8581 tttactcact ctctgccggt aatactggca tccagtcaat cggcgtctcg ccacgttgtt
     8641 ccagccactg atttgccagc acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa
     8701 gcggcgacgg atgcggtgct ttcagtacat gatggcgttg cttatctata atcgcccctt
     8761 tcttttgcgc atgtgatccc cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca
     8821 ggctgatgac tttatcagta aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac
     8881 ctgcgcgaac cgtcaacaca gtattgagca gcagaacgcc ctgacgcgcc cagctttcga
     8941 gatagccatg attagggcgg gtgaagcccg gaatagtatt ttccagctct ttatacatat
     9001 tcaataatga cggaggaatg gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct
     9061 gtcccggtcc gtgataagga tcctggccga gaatcaccac tttaacgtca cccaactcgg
     9121 taaagcggaa cgcgttaaag acatcttttt gtggtggata gatagtgacg ccggactgcc
     9181 gctcgctggc gacggtctga agggtattaa gaaaataagg ttgttgcttc tcttcagcca
     9241 gcacgtcatg ccaggttaat tcgttagcca tctcgctctc ctgcgaatct tcaatccgcc
     9301 tagcttaact gcttctttct acagaacaaa atcatgctca ttttttagac ttaaacgagt
     9361 gcaaaaaaat gtaaattggt aaaatttcac acccgcataa gttgatgtaa aacaataaaa
     9421 tccatttacg accacaatcg atgtgtggtt tttattgatt taaatcaaag aatcaagggt
     9481 ggttgaggag tatatataca ctcaagcaac aatggtttta ccaattggcc gcgacaggct
     9541 gaacaaatca aataattttg ccggggaggc atcacatgat tacaggtatc cagattacta
     9601 aagccgctaa cgacgatctg ctgaactctt tctggctgct ggacagcgaa aaaggcgaag
     9661 cacgttgcat cgttgcaaaa gcaggttatg cagaagatga agtggttgca gtaagcaaac
     9721 tgggtgacat tgaataccgt gaagttccag tagaagtgaa accagaagtt cgcgttgaag
     9781 gtggtcaaca cctgaacgtt aacgttctgc gtcgtgaaac tctggaagat gcagttaagc
     9841 atccggaaaa atatccgcag ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact
     9901 ctctgactcc ggaacagcag cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag
     9961 gcttaagtag caagttacta agcggaagag ggcgacctct tccgcttttt ttcgttaata
    10021 gaaaatgcgt accgcgcaat agaccagcaa cagggcaagc acgatattta actggcgacc
    10081 atactggcga aacaatcgct gaaacagatg ccccgccagc gcccagcaca cattgccaaa
    10141 cgtcccaatc atcgccagca aaacgctgac gccaactacc cagcttagcg cctgtgtttg
    10201 cggcagaaca aacgtcgaca gtgccgtaac accgtacaaa atgattttga cgttcacaaa
    10261 ctgcaaagca aagctggccc aaaagctgat tggttttgcc tgaagtccgt cttcctttgt
    10321 tgggctggtg gcgattttcc acgccagcca gacaatatat gccgcccccg cccaactcaa
    10381 aaggtgtacc gctgccgggt caatcactgc cagtgaaaat gaaatgcccg cacacagtaa
    10441 catcacaatc aaaaatccca gactcatccc tgccagcacg cgggtacttt gacgaaatcc
    10501 atgcgacgta gcagagctaa gggcgagaat attgttcggt cctggcgtca tagcggtaat
    10561 cagggtgtaa gtccaaaaag cacttaaaag ggtcggtgtc acttgttatc tcctctgttt
    10621 tggtagacag tacccgacac ggaacattta aaaaaatgaa tgtttttata ttctagattc
    10681 gatattttcg ataggtttgg ggttatggat ctgcgccgtt ttattacgct taaaaccgtg
    10741 gtggaagagg gttccttttt gcgagcttcg caaaaattgt gctgtacaca atcgacggtg
    10801 acttttcata ttcagcagct tgagcaggag ttttcagtcc agttatttga gaaaattggc
    10861 cgacggatgt gccttacccg cgaaggaaaa aagttgctgc cgcacattta tgaacttacc
    10921 cgagtgatgg atacgcttcg tgaggctgcc aaaaaggagt cagatccgga cggggaacta
    10981 cgcgttgttt cgggcgaaac gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt
    11041 cggcaacgcg caccaaaagt gcgtttatcg ttgcagtcgc tgaactgtta cgtgatccgt
    11101 gatgcgctac tgaatgatga ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat
    11161 gccctgaacc gacgagagtt gggtgaacaa tcgctggtgc tggtggcttc accgcaaatt
    11221 gcagatgtcg attttactga gccgggaaga cataacgcca gtagctttat tatcaacgaa
    11281 ccgcaatgtg tcttccggca gatatttgag agcacgctgc gtcagcggcg gatcacggtg
    11341 gaaaacacca ttgagctgtt aagcattgaa agtatcaaac gttgtgtcgc ggcgaatatc
    11401 ggtgtcagtt acttgccccg ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt
    11461 gaattgcctt ttggcgaaca gtcgcagacc attacggcca tgtgcgcaca ccatgctgga
    11521 aaagcggtta gcccggcgat gcacactttt attcagtgtg ttgaagagag ttttgtggct
    11581 ggataaaaat aatgtcggga ttaaacccgg catttaactt gtcgacaaag tgcgctttat
    11641 tcatgccgga tgcggcgtga acgccttatc cggcctacaa gatcgtgcaa attcaatata
    11701 ttgcatgag
//
LOCUS       DAZFWL020000142        11430 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFWL020000142 DAZFWL020000000
VERSION     DAZFWL020000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.142"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            130..747
                     /gene="yijF"
                     /locus_tag="ACHKNP_RS20345"
                     /old_locus_tag="pgaptmp_004069"
     CDS             130..747
                     /gene="yijF"
                     /locus_tag="ACHKNP_RS20345"
                     /old_locus_tag="pgaptmp_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HGU4706140.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(774..1679)
                     /gene="yijE"
                     /locus_tag="ACHKNP_RS20350"
                     /old_locus_tag="pgaptmp_004070"
     CDS             complement(774..1679)
                     /gene="yijE"
                     /locus_tag="ACHKNP_RS20350"
                     /old_locus_tag="pgaptmp_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HGU4706141.1"
                     /translation="MSVAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(1773..3953)
                     /gene="katG"
                     /locus_tag="ACHKNP_RS20355"
                     /old_locus_tag="pgaptmp_004071"
     CDS             complement(1773..3953)
                     /gene="katG"
                     /locus_tag="ACHKNP_RS20355"
                     /old_locus_tag="pgaptmp_004071"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HGU4706142.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(4282..5172)
                     /gene="metF"
                     /locus_tag="ACHKNP_RS20360"
                     /old_locus_tag="pgaptmp_004072"
     CDS             complement(4282..5172)
                     /gene="metF"
                     /locus_tag="ACHKNP_RS20360"
                     /old_locus_tag="pgaptmp_004072"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HGU4706143.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5248..5382
                     /locus_tag="ACHKNP_RS20365"
                     /old_locus_tag="pgaptmp_004073"
     CDS             5248..5382
                     /locus_tag="ACHKNP_RS20365"
                     /old_locus_tag="pgaptmp_004073"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706144.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(5521..7953)
                     /gene="metL"
                     /locus_tag="ACHKNP_RS20370"
                     /old_locus_tag="pgaptmp_004074"
     CDS             complement(5521..7953)
                     /gene="metL"
                     /locus_tag="ACHKNP_RS20370"
                     /old_locus_tag="pgaptmp_004074"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HGU4706145.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPVSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(7956..9116)
                     /gene="metB"
                     /locus_tag="ACHKNP_RS20375"
                     /old_locus_tag="pgaptmp_004075"
     CDS             complement(7956..9116)
                     /gene="metB"
                     /locus_tag="ACHKNP_RS20375"
                     /old_locus_tag="pgaptmp_004075"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGU4706146.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVMTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            9393..9710
                     /gene="metJ"
                     /locus_tag="ACHKNP_RS20380"
                     /old_locus_tag="pgaptmp_004076"
     CDS             9393..9710
                     /gene="metJ"
                     /locus_tag="ACHKNP_RS20380"
                     /old_locus_tag="pgaptmp_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HGU4706147.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            9894..10502
                     /gene="yiiX"
                     /locus_tag="ACHKNP_RS20385"
                     /old_locus_tag="pgaptmp_004077"
     CDS             9894..10502
                     /gene="yiiX"
                     /locus_tag="ACHKNP_RS20385"
                     /old_locus_tag="pgaptmp_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HGU4706148.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYIFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            10647..11322
                     /locus_tag="ACHKNP_RS20390"
                     /old_locus_tag="pgaptmp_004078"
                     /pseudo
     CDS             10647..11322
                     /locus_tag="ACHKNP_RS20390"
                     /old_locus_tag="pgaptmp_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2798 a         3188 c         2878 g         2566 t
ORIGIN      
        1 tctgtgccag taatctgaaa ctcctacgtt gttcaggagt tttttatccg cttctggaca
       61 ttttctctac agaacaatcg ttagcccgga aggtcgaatc tgtgactctt attttcacgg
      121 acgaaccaga tgaaagcttc actggcgctg ctcagcctgc taacggcatt taccagccac
      181 tccttaaaat ctcccgccgt tccaccaacc gtggtacaaa tccaggccaa taccaacctt
      241 gccatcgcag acggtgccag acagcaaatt ggcagcacgc tattttacga tccggcgtat
      301 atgcagctta cctatcctgg cggtgatgtc ccgcaagaac gcggggtatg ttccgatgtg
      361 gtgatccgtg cattgcgcag ccagaaggtc gatttgcaga aactggtgca cgaagatatg
      421 gcgaagaatt ttgccgagta cccgcaaaaa tggcagttaa agcgcccgga cagcaacatc
      481 gatcaccgtc gggtgcctaa tctggaaacc tggtttaccc gccacgataa aacacgcccc
      541 atcagcaaga accccagtga ctatcaagcg ggcgatattg tctcctggcg actggataac
      601 ggactggcac atattggggt ggtgtcagat ggcttcgccc gcgacggtac gccgctggtg
      661 atacataaca ttggcgcagg cgcgcaggaa gaagatgtgc tgtttagctg gcggatggtg
      721 gggcattatc gttattttgt taaataagcg atcgccccac atgaagaaaa aattcagatg
      781 ctttttacac tgacagcttc ttttttctta cggctcacca gcgccagtgc cagcacaatc
      841 agcacaatac cgctaccttc aacggccccc ggattctcac cgagcagcca ccaggaaaag
      901 agtacgccgc aaacgggaac ggccagcgtg cttaagctgg caatactggc aggcaagttt
      961 ttcaatacaa acaaccataa gctccacgcc agcgccgtcg ccagaatcgc actgtaggcc
     1021 agcgcccaga acacggtggg ctgccagtca atttcacgtt gcggcaccag taaagcgacc
     1081 acactcatca ccagcgccgc atagagcatc tgccaggatg ttaacgacaa taaatccacg
     1141 cgcggatgac gggcatacag acgtttagca acaatcgcgc tcgcccccca actgacgccg
     1201 gagaggattg ccagcatggc acttttcatc gaagagaaat cgagttgcca cggctgcaac
     1261 accaaaaata agccgaaagc ggcaatcagt atcgcgaaat attgcccacg tcgcaggcgt
     1321 tcaccgagaa acaacgcggc gaaaatcacc acccagaacg gcatggtata gcttaggatc
     1381 gccaccttcc ccgctccgcc gctgaccagc gcccactgcg ccaggccaac catcccgcag
     1441 gtttgtaaca gggcaatggc taaggtgtat ttaaacggtg tcgggcgcat tccgcgacca
     1501 cgtaataaaa ggacgatgaa taaaacgaga gcgccgaaaa tgcagcgtaa ggcggtaaag
     1561 tcgaaggcac cgatgtagct ggtgacttgc ttcatgaaaa tccagctata actccagata
     1621 agtgtgagca caaccaggcc actgattgcc agtgggttgc tctttcctgc gacagacatg
     1681 agagatccag tgtgtagttt tcctgtggac actatacgcc ggtaaagaaa cttcagcgac
     1741 tgcctgaaag gcagtcgctg aagaaggtca gattacagca ggtcgaaacg gtcgaggttc
     1801 atcactttca cccatgccgc cacgaagtct ttaacaaact tctcgtgagc atcgctgctg
     1861 gcgtaaactt cagccaccgc acgcaggaca gagttagaac caaataccag atccgcacgg
     1921 ctggcagtgt atttcacttc gccggtttcg cggtcacggc cttcaaacag ctctttcgat
     1981 tcgtcggtcg ctttccactc gtaacgcata tccagcaagt tcacgaagaa gtcattgctc
     2041 aatacgccaa cgcggtcagt gaagacgccg tttttgctgc catcgaagtt ggcacccagt
     2101 acacgcatcc cgcccaccag cgcggtcatt tccggcgcgg tcagggtcag ttgctgggct
     2161 ttgtcgatca gcagtgactc ggtggtggaa acatccagac gagcgcgata gttacggaaa
     2221 ccgtcagcaa ttggctccag cagctcgaac atctcaatgt cagtctgatc ctgacgcgca
     2281 tcaacgcggc ccggcgcaaa cggtacatga atgctcaaac ctgcggcgct tgcggctttc
     2341 tcaacaccaa ccacaccagc cagcacgatg atatccgcca gcgaggcttt accagactct
     2401 ttctggattt tctccaaaac aggcagagca cgaacggctg cggcgttcac atcccagtcg
     2461 cgctgcggca ttaatgccag acgcgcaccg ttggcaccac cgcgtttgtc gccaccacgg
     2521 aaggtagaag cagaagccca ggccaccgat accagctcac taacagacag accagaatcc
     2581 gcaatcgcga atttcagatc gataatgtcc tgctcggtcg ggttgtagat cggctgcggc
     2641 agcggatctt gccagatcag atcttctttc ggcacttccg gcccgatgta gcgagatttc
     2701 ggccccatat ccctgtgcgt cagtttgaac caggcacggg caaaggcttc gttgaacgcc
     2761 tgcggatcgt tgaggaaacg acgagagatc ttctcgaact caggatcaaa acgcagcgtc
     2821 aggtcggtca ccagcatcgt cggtttacgt ttcttcgacg gatcaaacgg atccgggata
     2881 atttccggtg cgtctaccgc ttcgaactgg attgcgccag ccgggctgcg ggtctgtacc
     2941 cactcatact tgaacaggtt ctcgaagaaa tagttgctcc actgggtcgg cgtctgggtc
     3001 cagactactt ccagaccaga ggtaatggca tctgcgccaa cgccgctgcc gtaagtgctc
     3061 gcccaaccta aaccttgttc ttcaatcggt gcagcttctg gatcaggacc tacatttgat
     3121 gtcggaccgg caccgtgggt tttacccagc gtatgaccac ccgcaataag cgccacggtt
     3181 tcttcgtcgt tcatgcccat gttgccgaag gtcgcacgga tagctgctgc cgcagaaagc
     3241 ggttcgccgc tgtgatccgg gccttccggg ttaacgtaaa tcaggcccat ctcggttgca
     3301 cccagcggtg ctttcgccag cgcttccgga tgacggtggg tcagccaggc tttttcatca
     3361 ccccagttaa catccagatc cggttcccag acgtcttcac gaccggcacc aaaaccgaag
     3421 gtacggaagc cggagttttc cagcgccacg ttacccgcga ggataaacag gtcggcccag
     3481 gagattttct gaccatattt ctgtttgatt ggccacaaca ggcgacgcgc tttatcgagg
     3541 cttacgttat ccggccagga gttcagcggt gcaaaacgtt gctgaccacg acccgcgccg
     3601 ccgcgtccat cgattgaacg gtaagtcccc gcgccgtgcc aggccatacg aataaacaga
     3661 ccggcgtagc tgccccagtc ggcaggccac cacggctgag attctgtcag cagggctttc
     3721 agatcttttt tcaggccgta gtaatctaat ttgctgaatt ctttgcggta gtcaaagtcc
     3781 tcacccagcg ggttagaacg attagaatgt tggtttaaca ggtcaacacg gagttgattt
     3841 ggccaccagt cgcgagtggt tgtgcccgcc cccgcgctct ggtcgtgacc gccctgatgg
     3901 aacgggcatt tgccagtggc tgtcgtgtta tgaatatcgt ctgacgtgct catcaatgtg
     3961 ctcccctcta cagtgttacc gttacgatac acagcgttag agagaagtta taattgaatt
     4021 aatccacgga tgcgatagtt gagatcttac atattgttgg ttaaagagat gtagatcaaa
     4081 ttgatcgtaa ttaagctttt ttgattatgt ccgaattcgg acaactgctt tataaaaaag
     4141 ggttttagat tcctattctt caaggaagga gggcgaaaac aaagctgaca accttatttt
     4201 tacgccctca tttcgaggca gcattttgtg ctctgtttaa aatttgtgat cacagtgtga
     4261 ttttcacaaa agccacacta tttataaacc aggtcgaaca cccagcgtat ggcaaatcgc
     4321 gtaactcatt tcagcgcggt taagcgtata gaagtggaaa tctttcactc cttcacggct
     4381 taaaatcttc accatatcca tggcaatatt cgcgcccacc agcttgcgcg tttcagcatc
     4441 gtcatccagc ccgtcaaaca tttgtgccat ccacgccgga atacgcacgt tggtcatatc
     4501 ggcaaatttc ttcgcctgtt taaagttaga taccggcaaa atccccggaa taatttccac
     4561 atcaatgccc gccgatacgc agcggtcacg aaaacgcagg tagctttcga catcaaagaa
     4621 gaactgagta atcgcgcggt tggctccggc atcgactttg cgtttcaggt tgagcaaatc
     4681 cgcctgagcg ctttttgctt ccgggtgaac ttccggatac gccgccacgg agatatcgaa
     4741 atctgccact tcttttaaca gcgtcaccag gtcagaagca tacatttccg gcttaccact
     4801 tcccggcggc agatcgccac gcagcgccac gatatgacga ataccgttat tccagtagtc
     4861 gcgtgcaatg gtgcgcagct cgtcgggcgt cgcatcaatg caagtaagat gcggtgccgc
     4921 ttccagacca gtgcgatctt taatgccttt aataatgctg tgcgtacggt cgcgctcgcc
     4981 ggagttcgcg ccataggtca ccgatacaaa cttcggtttc aggctgctaa ggcgatcgat
     5041 ggagttccac agggtctgct ccatttcact ggtacgcggc gggaaaaact cgaacgaaac
     5101 gttaatctgc ccctggactt ctgccaggct ctgattcagg gcatcccgct ggctggcgtg
     5161 aaaaaagctc ataccttacc tcatcaatcg cttgtagtta tatgttgtgt tgtgcacatc
     5221 tatccattta gacgtccaga tgtaaagatg aaggaaaagc cgaagggcgt caaccgaaaa
     5281 atgaccaatg atggtgagga aaaatcaggg aagatgaaaa aacttcagga tggtaagaaa
     5341 aagaaaatcc ggtatgcaaa gcaaaccgga ctgcaaatat aaactaacca tgtacttatt
     5401 gcttcttgcc ggattcgtcg taaacgcttc atccggccta atcctatgat aattcaatat
     5461 atttcattag aaaccataga cccgaaaaca tgagtaccgg gcattattaa atttctgaaa
     5521 ttacaacaac tgtgccagcc ggttgatatc cgactgaatc gccccggcgg tgacgtcgcg
     5581 cccagcgcca ggcccacgga tcaccagcgg attatcgcgg taccagcggc tttcgatggc
     5641 aaagacgtta tcgcatggca gcagtgatgc cagcggatgg tcttcacgca ccgcttccac
     5701 gcccacacgc gctttgccat tggcatcgaa acgcgccacg tagcgcagca ccagccccat
     5761 ttcgcgggcc gcttccagcc gttgcaccat ctgctcgtta agttcatcgc cattttcaaa
     5821 gaaatggtcg atgctgccgc cttcgcaatg agcaggcacc agcgattcca cacgtacctg
     5881 gtccggttca atgttgtaac ccgcttcacg cgccagaatt accagcttgc gcattacgtc
     5941 tttgccggag aggtcgtcac gcggatcagg ctcggttaag ccctgctgcc acgcctgatc
     6001 caccagctcg gtaaacggca cgctaccgtc gaattgcagg aacagccagg agagcgtgcc
     6061 ggagaagatc ccgctgatcg acaaaatagt atcgccgctg tcgatcagat cgcgcacggt
     6121 gtggttgatc ggcaagcccg caccgacggt ggcattgtac agccagtgac gcccggtttt
     6181 ttcgaaggcg tcgtggatct ggcgatattt attgctgtcg ctggctcccg ccagtttgtt
     6241 ggcgctgata acgtggaaac cgtggctggc gaaatcaaga tactgatcag caagctgctg
     6301 gctggcggta acgtccagca ccactaaatc atcatacgga tgggcgcgca tccacaggaa
     6361 caacgactct tcatcctgct caaccgcttc atcgttgaag aaggctaacg cgcggctggc
     6421 gtccagcccg tcatagctca acaggctgcg gcggctgtcc accacacctg ccagcacaaa
     6481 ctcaaagccg gtacgtgccg aaagcgtgct ctgctcacgg gcgaacagtt ccagccaacg
     6541 ggaaccgata ttgcccttac cgaacaatac caggccgatg cgtttttctg cgcggaagac
     6601 ggactgatgc agcccctgaa tcaggctttc ggtcgggccg gtgcgcagta ctgccaccag
     6661 gctgatgccg tcatcggact gccaggtaaa ttcgaccggc tggcctttca gttgctgcca
     6721 gaagcggtgg caatgcagcg ggttacgggt gacgcctgca ccgaccatcg ccaccagcgc
     6781 cagcccctga cgcaggcgca gttcgccagg taatcccgct tcgtcgagga ttttcagcgc
     6841 actgtcggcc acttctgagg tgtagcaaaa ttgcagcaac tggcgatcgt tatgtacgcc
     6901 aaccgccagc gggcgtacct gcgcgcgttt caggatttgg tcgatctctt tatgcgccag
     6961 tttgaaatcc tgactgacgg gcacctgaaa ctcaatcaaa cagacatcat cgtggctggt
     7021 gacaatacgc gcaccagtac cggaggccag cacgcgttca atgcgcgtgg aaccttgatc
     7081 cggcgtgtag ctacagcgca gttgcaggtc gatttcgctg ccagaaaccg gctgtaaagt
     7141 acgggcgtga agaacgggag ccgccaggcg cgccagttcg ctggcctcat ccagacgcag
     7201 caacggcagc aggcaggcat ctttcacttt acgcgggtcg gcactgtata ccccggcgac
     7261 gtcgctccag atggttacgc gagaaacacc cgccagcgca ccgatttgtg tcgcggaata
     7321 gtcggaaccg ttacgcccca gcagcaccgt ttcaccggcg ttgttgcggc tgataaatcc
     7381 ggtcaccacc agacgtttgc ccggatgttg caccagcagc tgttgcagca acgggtaaga
     7441 aagcccttca tcaacctgcg gttgtgcggc gcgttcagcg cgtaaaaact cgcgggcatc
     7501 aagccaggcc gctggcagcc cttgttgatt aagtaccgca gacatcaggc gtgccgacca
     7561 tacttccccg tggcccacca cttccgcata cactgcgtcg ttaataccgc tgtcgagcag
     7621 cgccgccagg cgctcaaggt cgctgacaaa agcgctaatg aggctatcgg cttcttcagc
     7681 gggtagcaga ccgctaatca gatcgcactg atagcgacgc agcgtttgtt gaacctgatg
     7741 cgcagagaga cgatcggtct ggcttagttt caaccagtta atcaactggt tagtggtgct
     7801 accggcggcg gaaaccacca tcatatcgtc aggctgagag tactccgcca taatgcccgc
     7861 gacacgcaaa taacacttca catcagccag actactgcca ccaaatttat gcagctgacg
     7921 acctttcgcc cctgcctgcg caatcacact catttttacc ccttgtttgc agcccggaag
     7981 ccattttcca agtcggcaat taaatcttcg ccatcttcaa taccggtgga gatacgcagc
     8041 agcgtctcgg aaatcccggc ggcagcacgc gcttctggtg ccatgcctgc atgtgtcatg
     8101 gttgcggcgt gagagattaa actttccact ccccctaatg attccgccag cgtaaacaac
     8161 gacagcccgc ccaggaaacg acgcagcgtc tgctcatcgc catccagttc aaaactcaac
     8221 attgcgccaa agcctttttg ctgacgcgcg gcaatttcat gcccctgatt ttccggcagc
     8281 gacgggtgat acagtttttt caccaacggc tgggtttgca gatatttcac aatcgcctgc
     8341 gcgttgcgct gcgccagctc catacgcggc accagcgttc gcaacccgcg tagcagcaga
     8401 tagctgtcaa acgcgccgcc cgtcacgcca atattgtttg cccaccaggc aagttcagtg
     8461 acaacgtccg ggtctttagc aatcaccacg ccagccacca cgtcagagtg accgttcaga
     8521 tatttcgtac atgaatgcaa caccagatcg gcacccaatg ccagcggatt ttgtaatgcc
     8581 gggcttaaga aggtgttatc caccacgctc accgccccga cttcccttgc cagatggcag
     8641 attttcgcaa tatccacgac gcgtaacaat ggattacttg ggctttctac cagtaccagt
     8701 ttgggttttt ctgccagtgc tgcccgtaat gcctgttcat cgccttgatc aacaaacaac
     8761 acgcgatagc aaccgcgttt cgccaggctg tcgaacaggc gatagctacc gccgtagcag
     8821 tcgtgcggcg caaccagcag atcgccaggt ttcaaaaaga cggtcattac caggtgaatc
     8881 gcggacatgc cggtattagt aagtactgca ccagcaccgc cttccagttc tgccagcgca
     8941 cgctgaacca catcgcgcgt tgggttgccg cgacgcgagt aatcatgcgc gcgcggttca
     9001 ttaaatccgg taaagttata tgtgctggaa agatggatcg gtgggacaac gcaaccatac
     9061 tgttcgtcgt catttaaccc gctacgcact gcgatggtgg cctgtttacg cgtcatgtga
     9121 tgaagttccc tgggctttgt cggtgaaatg tcaggcacca gagtaaacat tgtgttaatg
     9181 gacgtcaata catctggaca tctaaacttc tttgcgtata gattgagcaa atcccaaata
     9241 gccgttaaaa ttatatgcat tatcacgccg acaggtgcat tacacgatgt cacggtaacg
     9301 cctgtacggt aaactatgcg ggtttacggt cagtacccac ataaattgtg tggtctggtc
     9361 tcaatttatt gacgaagagg attaagtatc tcatggctga atggagcggc gaatatatca
     9421 gcccatacgc tgagcacggc aagaagagtg aacaagtcaa aaagattacg gtttccattc
     9481 ctcttaaggt gttaaaaatc ctcaccgatg aacgcacgcg tcgtcaggtg aacaacctgc
     9541 gtcacgctac caacagcgag ctgctgtgcg aagcgtttct gcatgccttt accgggcaac
     9601 ctttgccgga tgatgccgat ctgcgtaaag agcgcagcga cgaaatcccg gaagcggcaa
     9661 aagagatcat gcgtgagatg gggattaacc cggagacgtg ggaatactaa agcggaaatg
     9721 agcggcgcgg agtggaatcg cctgatgcgc tacgcttatc aggcctacgt catattgcaa
     9781 tttattgaat ttgcacgaac ttgtaggccg gataaggcgt tcacgccgca tccggcataa
     9841 acaacgagca cgttgtctgc gacccaccgc tttttataca tggacgttta actatgaaaa
     9901 acaggctgct gatcctcagc ctgctggttt ctgtacctgc ctttgcctgg cagccgcaaa
     9961 ctggcgacat catctttcaa atctctcgct catcgcaaag taaagcgatc caactggcaa
    10021 cccattccga ttacagccac accggtatgc tggtgatgcg taacaaaaag ccctacattt
    10081 ttgaagcggt cggcccggtg aaatacaccc cgctcaagca gtggattgcc catggtgaaa
    10141 agggcaaata cgtcgttcgc cgcgttgaag gcggactgag tgttgaacaa cagcaaaaac
    10201 tggcgcaaac ggcaaaacgt tatctcggta aaccgtatga tttcagcttc tcatggagcg
    10261 acgatcgcca gtactgttcg gaagtggtgt ggaaggttta ccagaacgcg ctgggaatgc
    10321 gcgtgggcga acagcagaag ttgaaagagt ttgatctcag caacccgctg gtccaggcga
    10381 agctcaaaga acgctacggt aaaaatattc cgcttgagga aacggtagtt tcaccacaag
    10441 ccgttttcga cgcgccacaa ctcaccacgg ttgccaaaga gtggccgctg ttttcgtggt
    10501 aatggatgat gaaaaaaata attcccgccg tagtctgttt gttgctttgt agttgcacga
    10561 cctttccgca agctgtcgct ccggtaaacg gtgatgcctc caattagttg aattaaggat
    10621 ataatactcg cttttaaggt tgtttcatgg tcacagttaa ccttcattgc ccccgttgcc
    10681 agtccgttca ggtttaccgt cacgggcaga acccaaaaag acatgatcga ttccgcggcc
    10741 gtgactgtca ccgggtattt cagctcagtt actgttacga gccccgtaaa ccgggagtta
    10801 aagaccaaat cgctgaaatg actttcaacg gtgccggggt tcgcgatact gcccgtacgc
    10861 tgaaaatcgg cattaataca gtcatccgaa ctttaaaaaa ctctcgccac gaagaataac
    10921 gtcatccccg gtcgctcatg ctgatgtggc actcatctgc aaacttagtc agcaatggag
    10981 ctttgtcggc agtaaagcgc gacagcactg gctctggtac gtgtacaaca cgaaaactgg
    11041 tggtgttctg gcctacactt ttggcccccg gactgacgaa acctgtcgtg aattgcgggc
    11101 gctgcttacc cttttaccat cggcatgttg accactgacg aatggggtag ttacgctaga
    11161 gagttaccga aggagaagca tctgactggt aagattttta ctcagcacat tgagcgtaac
    11221 aatctgaccc tgagaacccg aatcaaacgt ctggcccgca gaaccatctg tttctcgcgt
    11281 tctgtagaac ttcatgaaaa agtcatcggt gcttttatcg aaaatatatg ttctactaat
    11341 tggagtcatc acccttctga taagctttcc ttggatgatt accagactga atacacagcc
    11401 aaagattttt acctcattct gctctttttt
//
LOCUS       DAZFWL020000143        11190 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFWL020000143 DAZFWL020000000
VERSION     DAZFWL020000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.143"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            22..537
                     /gene="ehxC"
                     /locus_tag="ACHKNP_RS20395"
                     /old_locus_tag="pgaptmp_004079"
                     /gene_synonym="hlyC"
     CDS             22..537
                     /gene="ehxC"
                     /locus_tag="ACHKNP_RS20395"
                     /old_locus_tag="pgaptmp_004079"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HGU4706149.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            539..3535
                     /gene="ehxA"
                     /locus_tag="ACHKNP_RS20400"
                     /old_locus_tag="pgaptmp_004080"
     CDS             539..3535
                     /gene="ehxA"
                     /locus_tag="ACHKNP_RS20400"
                     /old_locus_tag="pgaptmp_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HGU4706150.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGRTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGSLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARMISELGDKDDVVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            3585..5705
                     /gene="ehxB"
                     /locus_tag="ACHKNP_RS20405"
                     /old_locus_tag="pgaptmp_004081"
                     /gene_synonym="hlyB"
     CDS             3585..5705
                     /gene="ehxB"
                     /locus_tag="ACHKNP_RS20405"
                     /old_locus_tag="pgaptmp_004081"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HGU4706151.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELSAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            5709..7147
                     /gene="ehxD"
                     /locus_tag="ACHKNP_RS20410"
                     /old_locus_tag="pgaptmp_004082"
                     /pseudo
     CDS             5709..7147
                     /gene="ehxD"
                     /locus_tag="ACHKNP_RS20410"
                     /old_locus_tag="pgaptmp_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
     gene            7214..7357
                     /locus_tag="ACHKNP_RS20415"
                     /old_locus_tag="pgaptmp_004083"
                     /pseudo
     CDS             7214..7357
                     /locus_tag="ACHKNP_RS20415"
                     /old_locus_tag="pgaptmp_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            7361..7552
                     /locus_tag="ACHKNP_RS20420"
                     /old_locus_tag="pgaptmp_004084"
                     /pseudo
     CDS             7361..7552
                     /locus_tag="ACHKNP_RS20420"
                     /old_locus_tag="pgaptmp_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
     gene            complement(7560..7913)
                     /gene="cmi"
                     /locus_tag="ACHKNP_RS20425"
                     /old_locus_tag="pgaptmp_004085"
     CDS             complement(7560..7913)
                     /gene="cmi"
                     /locus_tag="ACHKNP_RS20425"
                     /old_locus_tag="pgaptmp_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HGU4706152.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GIYHCEEGYVEYAND"
     gene            complement(8086..8868)
                     /locus_tag="ACHKNP_RS20430"
                     /old_locus_tag="pgaptmp_004086"
     CDS             complement(8086..8868)
                     /locus_tag="ACHKNP_RS20430"
                     /old_locus_tag="pgaptmp_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGU4706153.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGNFSLTPPYPFVQLATLKQR
                     TEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNKHA"
     gene            complement(8870..9283)
                     /locus_tag="ACHKNP_RS20435"
                     /old_locus_tag="pgaptmp_004087"
     CDS             complement(8870..9283)
                     /locus_tag="ACHKNP_RS20435"
                     /old_locus_tag="pgaptmp_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706154.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            9843..10073
                     /gene="vapB"
                     /locus_tag="ACHKNP_RS20440"
                     /old_locus_tag="pgaptmp_004088"
     CDS             9843..10073
                     /gene="vapB"
                     /locus_tag="ACHKNP_RS20440"
                     /old_locus_tag="pgaptmp_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HGU4706155.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            10070..10486
                     /locus_tag="ACHKNP_RS20445"
                     /old_locus_tag="pgaptmp_004089"
     CDS             10070..10486
                     /locus_tag="ACHKNP_RS20445"
                     /old_locus_tag="pgaptmp_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4706156.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRAAVDATTKIKVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(10648..>11190)
                     /locus_tag="ACHKNP_RS20450"
                     /old_locus_tag="pgaptmp_004090"
     CDS             complement(10648..>11190)
                     /locus_tag="ACHKNP_RS20450"
                     /old_locus_tag="pgaptmp_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706157.1"
                     /translation="QICKSITASADCEEIIKLIYLRRYFEIVDERGDAYQLLSNLFHR
                     RVAPLDYREPAAAGSGYPKMAPEKIQQALRDIREYVDSFDYPRLQALVSSPDEIKNLY
                     RRCRNGYEKLQVFRLLELDQDHPVIRKFVNETYHIENEFICQLDPSRFDLIPEYVIME
                     CDKLIALPPAANQSSVARIA"
BASE COUNT         3359 a         2120 c         2580 g         3131 t
ORIGIN      
        1 tttcttattt atttaaacat tatgaaatct aatgcttttg acgtcatggg gaaggttgca
       61 tggctatggg cctgttctcc tctgcataaa aaatggccat tatctgtttt tgctattaat
      121 gtcatacctg ctattcagac aaatcaattt gctctcttaa taaaggatga gcttcctgta
      181 gctttttgta gctgggccag tttagatctg gaatgtgagg taaaatatat aaatgatgta
      241 acatctttgt atgcaaaaga ctggatgtcg ggagaaagaa agtggtttat tgactggata
      301 gctccatttg gacacaacat ggagttatat aaatacatga ggaaaaagta tccttatgag
      361 ttattcagag caataaggtt agatgagagc agtaaaacag gaaaaatagc tgaatttcat
      421 ggagggggga tagataaaaa actggcatca aaaatattca ggcaatacca tcatgaactt
      481 atgtctgaag taaaaaacag acaagatttt aattttaata ttgagaaaga aaactaatat
      541 gacagtaaat aaaataaaga acattttcaa taatgcgaca ttgactacaa aatcagcatt
      601 taatacagca tcatcaagcg tacgttccgc tggcaaaaaa ctcatattat taatacctga
      661 taattatgaa gctcagggcg tgggtattaa tgagttggtc aaagctgctg atgagcttgg
      721 aatagaaata caccgtactg aacgagatga tacagcgatt gcaaaccagt tttttggtgc
      781 agcagaaaaa gttgtaggat taactgaacg tggtgttgca atattcgcac cacaacttga
      841 caaacttctg cagaagtatc agaaagttgg gagtaaaata ggaagaaccg ctgaaaatgt
      901 aggtaataat ctgggaaaag ccggaacagt tctctcagca ctacagaatt ttacggggat
      961 tgctttatca ggcatggctc ttgatgaatt gctgagaaaa caacgggaag gagaggatat
     1021 aagtcagaat gatattgcca aaagtagtat tgaacttatt aatcagcttg tagatacagt
     1081 atcaagtata aacagtaccg ttgattcatt ttctgagcag cttaaccagc ttggctcatt
     1141 tttatccagt aaacctcgct taagttctgt tggtgggaaa ttacaaaatt taccagacct
     1201 gggctccctg ggggatgggc tggatgttgt ctccggaatt ctttctgctg tatcagcaag
     1261 ctttattctg ggaaacagtg acgcacatac aggaacaaaa gctgcagcgg gtatcgaact
     1321 gacaactcag gttcttggaa atgttggtaa agctgtttcg caatatattc tggctcagag
     1381 aatggcacag gggttatcga caacagctgc aagtgcgggt ctgatcacat cggctgttat
     1441 gctggctatc agtcctcttt ctttcctggc tgctgcagat aaatttgagc gagctaagca
     1501 gcttgaatca tattctgaac gatttaaaaa attgaattat gaaggggatg ctttactcgc
     1561 agcctttcat aaagaaaccg gagctataga tgcagccctg acaacaataa atactgtcct
     1621 gagttctgta tctgcgggag ttagtgcagc ctccagtgca tccctcatag gggccccgat
     1681 aagcatgctg gtgagtgcat taaccggtac gatatctggc attctggaag catcaaaaca
     1741 ggctatgttt gagcacgttg cagagaaatt cgctgctcgg atcaatgaat gggaaaagga
     1801 gcatggcaaa aattattttg agaatggcta tgacgcaaga catgctgcgt ttttagaaga
     1861 ctctctgtct ttgcttgctg atttttctcg tcagcatgca gtagaaagag cagtcgcaat
     1921 aacccagcaa cattgggatg agaagatcgg tgaacttgca ggcataaccc gtaatgctga
     1981 tcgcagtcag agtggtaagg catatattaa ttatctggaa aatggagggc ttttagaggc
     2041 tcaaccgaaa gagtttacac aacaagtgtt tgatcctcaa aaagggacca tagacctttc
     2101 aacaggtaat gtatcaagtg ttttgacatt tataacacca acatttaccc caggagaaga
     2161 agttagagaa agaaaacaga gtggtaaata tgaatatatg acatctctta ttgtaaatgg
     2221 taaggataca tggtctgtaa aaggcataaa aaatcataaa ggtgtatatg attattcaaa
     2281 attgattcag tttgttgaaa agaataacaa acactatcag gcgagaatga tttctgaact
     2341 cggagataaa gacgatgtgg tttattctgg agcaggctca tcagaagtat ttgctggtga
     2401 aggtcatgat accgtatctt ataataagac ggatgttggt aaactaacaa ttgatgcaac
     2461 aggagcatca aaacctggtg agtatatagt ttcaaaaaat atgtatggtg acgtgaaggt
     2521 attgcaggaa gtcgttaagg aacaggaggt gtcagtaggg aagcgaacag agaaaataca
     2581 atatcgtgat tttgaattca gaaccggtgg aattccttat gatgtaatag ataatcttca
     2641 ttctgttgaa gagctcattg gcggaaaaca tgatgatgaa ttcaaaggcg gtaagtttaa
     2701 tgatatattc catggcgcag atgggaacga ttatatcgaa ggtaattatg gtaatgatcg
     2761 actatacggc gatgatgggg atgattatat atccggagga cagggagacg accagttatt
     2821 tggtggtagt ggaaacgata aattgagtgg aggggatggt aataattatc tgacaggagg
     2881 aagcggtaat gatgagcttc aggcacacgg agcttataat attctgtcag gtggtactgg
     2941 tgatgataaa ctttatggtg gtggtggtat tgatcttctg gatggagggg aaggtaatga
     3001 ctatctgaat ggtggttttg gtaatgatat ttatgtttat aggcaaaact atggtcatca
     3061 tacaattgca gatgaaggag gtaaaggaga tcgtttgcac ttatctgata ttagctttga
     3121 tgatatcgca tttaagagag ttggaaatga tcttatcatg aataaagcca ttaatggtgt
     3181 actttcattt aatgagtcaa atgatgtcaa tgggataaca tttaaaaact ggtttgcgaa
     3241 agatgcctca ggagcagata atcatcttgt tgaggttata acagataaag atggtcgaga
     3301 gataaaagtt gataaaatac ctcataataa taatgaacgg tcaggttata taaaagccag
     3361 taatatagca tctgaaaaaa acatggttaa tatcaccagt gttgccaatg atattaataa
     3421 gattatttct tcagtttcag ggttcgattc aggtgatgaa cgattagcat ctttatataa
     3481 tttatcctta catcaaaaca acacacactc aacaacttta acgacaactg tctgagacta
     3541 aaagaggagg tgatggccat tccaggagga atggtcatcg ttatatgatg agtaaatgta
     3601 gttctcataa tagtctgtat gcactgatat tgcttgcaca atatcataat ataactgtca
     3661 atgctgaaac tataaggcat cagtataata cccacacaca agattttggg gtgactgaat
     3721 ggttactggc agcgaaatcc attggcttaa aagcaaaata tgtagaaaaa catttttcca
     3781 gattgtcaat aatttcttta cctgcgttga tatggcggga tgacggtaag cattatatat
     3841 tgtctcgtgt tactaaagat tcatcacgct atcttgttta tgatccagaa caacatcagt
     3901 cactaacttt tagtcgggat gagtttgaaa aactgtatca gggaaaagtc attctggtta
     3961 cgtcaagagc aacagtagtc ggagagttag ctaaatttga tttttcttgg tttatcccct
     4021 ctgttgtgaa atacaggagg attttacttg aggtgttaac tgtttctgct tttattcagt
     4081 ttcttgcgtt aataacacct cttttttttc aggttgtaat ggataaggtt ttagttcacc
     4141 gggggttttc aacgttaaat attatcacaa tagcatttat tatagtgata ctttttgaag
     4201 tgatattaac cggagccaga acttatattt tctctcatac tacaagtcgt attgacgtcg
     4261 aactgagcgc taagttattc agacatttgc ttgcattgcc tgtttcatat tttgaaaatc
     4321 gcagggtcgg agagaccgtt gccagagtaa gggaactgga gcaaattcgt aattttttaa
     4381 ccggacaagc gttgacatca gttcttgatc tatttttttc tgtaatattt ttttgtgtca
     4441 tgtggtatta cagccctcaa ttaacactgg ttatattatt gtcactacct tgttatgtta
     4501 tatggtcatt gtttatatca cccttattac gtcgacgtct tgatgataag tttctcagga
     4561 atgcagaaaa tcaagctttt cttgtcgaaa cggtaacagc aataaataca attaaatcca
     4621 tggcagtatc accacagatg attgctacat gggataaaca actggccggg tatgttgctt
     4681 ccagtttcag ggtaaatctg gttgcaatga cagggcagca ggggatacag ctgatacaga
     4741 aaagtgtaat ggtaattagt ctatggatgg gagcacatct tgtcatatcg ggagagataa
     4801 gtatcgggca gttaatcgcg tttaatatgc ttgcaggtca ggtcattgct cctgttatca
     4861 gactggctca tctttggcag gattttcagc aggttggtat ttctgttgag cgccttggtg
     4921 atgtattaaa tacaccggta gagaaaaagt caggcagaaa tatactgccg gaaattcagg
     4981 gggatatcga atttaaaaat gtcagatttc ggtattcttc tgacggtaat gttattttga
     5041 ataatattaa tttatacata tcaaaggggg atgttatcgg tatagttggc cgttctggtt
     5101 caggaaaaag tacattaaca aaactgcttc agcgctttta tataccagag accggacaga
     5161 ttttaattga tgggcatgat ttatcacttg cagatccaga atggttacga cgccagattg
     5221 gtgttgtatt gcaggaaaat atactattaa atcgtagtat tatcgataat attacattag
     5281 cttctccggc tgtatctatg gaacaggcta ttgaggcagc cagacttgca ggtgcccatg
     5341 attttattac agaactaaaa gaagggtaca atactattgt tggagaacag ggcgttggtc
     5401 tttcaggagg gcagcgccaa cggatcgcaa tagcccgggc tcttgttaca aatcctcgaa
     5461 ttcttatttt tgatgaagca accagtgctc ttgattatga gtcggaaaat ataataatga
     5521 aaaatatgtc aagaatatgt aagaacagaa ccgtaattat tattgcacac aggttgtcaa
     5581 ctgtaaaaaa tgcaaacaga ataattgtta tggataatgg ctttatttct gaagatggca
     5641 cacataaaga gcttatctcc aaaaaagaca gtttatatgc atatttatat cagttgcagg
     5701 cataacgtat gcgcttttac atgaaaggtt tatgggattt ggtttgccgt tataagacgg
     5761 ttttttctga tgtctggaaa attcgtcaca cacttgatgc tcctgtgaga gaaaaagatg
     5821 agtatgcatt tcttcctgct caccttgaac tcattgaaac accagtatcc agacgttctc
     5881 attttgttgt atggagtatt ttattatttg taattatatc tcttctctta tctgttcttg
     5941 ggaaagttga agtggtttca gtagcaaatg gtaagtttac tcatagtgga agaagcaaag
     6001 aaataaaacc gatagagaat gcgattgtag aaaaaataat ggtaaaagat ggctcttttg
     6061 taaaaaaaat gatccattag ttgaattgac ggtgcctggt gttgaatctg atattttaaa
     6121 atcagaagca tctttgttgt atgaaaaaac agaacaatat cgctacgcaa ttctttctga
     6181 atcaatacaa cggaatgagc ttcctgaaat aaggataaca gattttcctg gcggagaaga
     6241 taacgcggga ggtgaacatt ttcagagggt tagctcatta ataaaagagc aattcatgac
     6301 atggcagaac agaaaaaatc aaaagcagct cacattaaat aaaaaaatag ttgaacggga
     6361 tgcagcgctt gctcgtgtta gcctgtatga gcatcaggta tcacaggaag gaagaaaact
     6421 caatgatttt aagtatttgt tgaataaaaa agctgtttct caacattcag ttatggagca
     6481 ggaaaatagc tatattcagg caaaaaatga acatgcagtc tggcttgcac aggtttctca
     6541 acttgaaaaa gaaatagaac ttgtgcggga agaactggca ctggagacga atatcttcag
     6601 aagtgaaatt atcgagaagc acagaaaatc aacagataac attgtgttgc tggagcatga
     6661 gcttgaaaaa aacaggcaga gaaaagcatc atcttttatt aaagctcctg tgagtggtac
     6721 tgttcaggag ttaaatatac atacagaagg tggtgttgtc acaacagcag aaacgctgat
     6781 gattattgtc cctgataatg atattctcga agtaacagca tctgtactta acaaggatat
     6841 cggttttata caacctggac aggaggtcgt tattaaagta gatgcatatc catatacacg
     6901 tcatggttac cttacaggga aagtaaaaaa tataactgca gattctgttt ctgttccgga
     6961 tacagggctt gtatttaacg tgattatatc tgttgatcgg aatgatatac agggagaaag
     7021 aaaaaaaatc cctgttacag ctggaatgac tgttatggca gaaataaaaa ccggggtgcg
     7081 tagtgttatc agctatcttc ttagtccatt aaaggaaact attaatgaaa gtttacgtga
     7141 acgttagtat ataaataaca tattggcttt ttatatgata tagatttatg aaataatctc
     7201 gtaaagatta tctatgttat ttaaacaagc tatgatacag tttttatctt taaaaagagt
     7261 taatggaatg gatagtatta aattacttga taaagttatt atctatcaac aacagaatga
     7321 gcaattatat ccagcacagg aacatcttct tttacaatgt tcatatgatg ataaatacga
     7381 ttttaacgcc agcactcacc gtggcatcat cggagagtcg ctcacaaggc tcggggcgat
     7441 gttttctggt aaagagtacc agatactgct tcctggtgaa attcacatta aagaaagtgg
     7501 taagcgagaa tataagggcc gggattccgg cctttttatg cagcctttat aacagtgtat
     7561 tagtcattcg catattcaac atatccctcc tcacaatggt atatgccata atacattctc
     7621 ttaccctccg ggccttcagc tttaaatggg acaagaagtg ttttttcatc ttcaacatct
     7681 gttaaacttc tctcaatctt ttcccatgta attttcggga tttctgtacc aagaagttga
     7741 acatcgttgt cccagcgttt cattctttgc aggtaatcat tttttacaaa cgttctggcc
     7801 tgttcatcgc tgacctgata gcatgccgga cctttatctt cagaccagaa aaaaacagca
     7861 gcaagagcaa taatcgttaa aataaaaata aatttcatgc taattacttt cataaaatct
     7921 ccacaacttc tgtcgttcat tcgatataaa aaaacattac gaatatatcc gtacaatgtg
     7981 agcatactgt gaagtggctt taacaattat gaaataatac aagagatcac atattgcagt
     8041 ggtctctttt ttatagaaga aatcacaatt cataattgtg acttattatg catgtttgtt
     8101 tttcagcatt gtgaccgcat cggactcagg catcgaaaac tgcacccggt gccgggcagc
     8161 gacatccagc gcaaacacct tcgtgtagac ctccgttgag ctgatggact tatgccccat
     8221 cagactctgc aggaccttca gcggtatacc ggcatacagc atatgcatcg cataggaatg
     8281 gcggaacgta tgtggcgtga ccgggacaga gaacgtcaca ccatcagcgg cagcggcggc
     8341 aaccgcctcc ccaatccagg tcctgaccgt tctgtccgtc acttcccaga tccgcgcttt
     8401 ctctgtcctg cctgttcgtt tattacgccg ttccatgggg attttcaatg tggccaccat
     8461 cgtctgcagc tggctgacgt accaggagtc ggagagcgga accagccggt gagtctgctg
     8521 cccggcgggc gttcttcctg ccgtcctggc ggctttttcg gtccgttgtt tcagggtcgc
     8581 aagctgcaca aacggatacg gaggcgtgag cgaaaaattc ccccgcgtca gcgccagtgc
     8641 ttcattaatg cgcgcaccgg tgttccacag tgtggccagc agcatcttgc ggcgcagatc
     8701 cgggacgtaa tggagcaggg cgctcacttc aggggccagc aggtatttgg gcagttcatc
     8761 atgaaccatc gacatctggc ggagtgccag cgctgccgga taatcgatgg caacaggaag
     8821 ctgagcggca tcccgggcat ggtggatcgg tgccggcagg tgctgcatat cagtgttcac
     8881 tccacgggga aacgtcgggc aggttttcca caacccggta ctttcccgga taacggagct
     8941 ggttaccgcc acgcgtccag acggtgacaa caaaaccccc tccctggttg ttttcgggga
     9001 gcaggcgatc gcactgctca ctcagcatgt ggatcacgaa cggcgcatcg ctgccgtctt
     9061 caaatatcag ttccagcgca tcgcggccat gcagcgttcc cttactgatg atgacgtact
     9121 cagccccccg catctccctg agtaaatgtt ttgccgcatc cgggacaagc agcctggctg
     9181 cgccggcatt ccagctgagg tagacatatc cggcctgcgc ctgtacagac tgccagtaat
     9241 ccgttctggc gacagcctga ccttcattct caataaaaaa catgggtgac tccttttttc
     9301 ttttccgggc agggacggtc atgcgataac gtcatccatc atcactgctc ttaccgtcac
     9361 gccatgtgat aacatcatgc cgttatcatc atcaccatga taacaagcat ccatataacc
     9421 acgctgttaa attaccggag gaccatttcc gacagatccg atttttctgt tattgacggg
     9481 ttttcggaag tgtatcacgc tgaggaaata atcatatcgc tgttttccgc ccctgcgcga
     9541 cgcatcacag gtcagggaga tgacacaaca tatagtgtca tcagcagatg aatccggcac
     9601 cacatgttgt gatgacggga ggtggcgttg tgtgggggga gaggaagatc ctgaacaacc
     9661 ttttttcgga tctccgataa aaaagaaaga aatagagata tgctaaacat atgcctggca
     9721 tctacatatg ctaccgctat acatatgcca cacatctgcc tatactaaac atatgcacac
     9781 acccggtgct ttgaatattc cggatatgtt tcacatatcc atatgctcgg gagagaaaga
     9841 acatgagaac cgtatcgata tttaaaaatg gcaacaaccg cgccatccgt ctgcctcgtg
     9901 atctggattt tgagggggtg agcgagctcg agatcgtccg ggaaggggac agcatcatcc
     9961 tgcgccccgt ccggccaacc tggggatcat tcctagagta cgaaaaagcc gatccagatt
    10021 ttatggcgga gcgcgaggat gttgtcagcg acgaagggcg atttaacctg tgaacaagac
    10081 ttatatgctt gatacctgta tctgctcgtt catcatgcgt gaacagccag aagccgtcct
    10141 gaagcgtctg gagcaggcgg tgctacgcgg ccaccgcata gtggtctcgg ccatcaccta
    10201 ctctgaaatg cgcttcgggg ctaccggccc aaaggcctca ccacgtcacg tccaactggt
    10261 cgatgaattc tgcgcccgcc tcgatgccat cctgccctgg gatcgcgccg cagtagacgc
    10321 caccacgaag attaaagtgg cactgcggct ggccgggacg ccgatcggcc cgaacgacac
    10381 ggcgattgcc gggcacgcca tcgccgccgg ggcgatactg gtaacgaata atacaagaga
    10441 atttgagcgg gtgcctgacc tggtgctgga agactgggtg aagtaagaca ctcttttcaa
    10501 agtcaggaga taagggcacc aagaaacagc ccgtatacgg gctgttttaa cagatatcta
    10561 cagtaagcgg tcctaaactg tagggtatct tcgaagatca gactatcagg gagctaaagc
    10621 ctatcttccg gcggcaaaaa cagtggctca agcgatacgg gcaaccgagc tctggtttgc
    10681 tgccggtggc agggcgatga gcttgtcaca ttccataatc acgtactccg ggatgagatc
    10741 gaatctggac ggatctagtt gacagatgaa ctcattttca atgtggtacg tttcgttaac
    10801 gaattttcgt attaccggat ggtcctgatc cagttccagt aaacggaata cctgcagttt
    10861 ttcatagccg ttacgacagc gacggtacag gttttttatt tcatctgggc tgctgacgag
    10921 agcctgaagt cgcgggtaat caaaactgtc cacatactcc cggatatccc gcaaggcctg
    10981 ctgaattttc tcaggagcca ttttggggta gcccgagccc gcagcggcag gctcgcgata
    11041 atccagcggg gcgacgcgtc gatggaatag attggataga agctgataag catcaccacg
    11101 ttcgtcaacg atttcaaagt agcgacgaag atagatgagc ttaatgatct cctcacagtc
    11161 tgctgacgca gtgatggatt tgcagatctg
//
LOCUS       DAZFWL020000144        10947 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFWL020000144 DAZFWL020000000
VERSION     DAZFWL020000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.144"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            57..818
                     /gene="adiY"
                     /locus_tag="ACHKNP_RS20455"
                     /old_locus_tag="pgaptmp_004091"
     CDS             57..818
                     /gene="adiY"
                     /locus_tag="ACHKNP_RS20455"
                     /old_locus_tag="pgaptmp_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HGU4706158.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            955..2292
                     /gene="adiC"
                     /locus_tag="ACHKNP_RS20460"
                     /old_locus_tag="pgaptmp_004092"
     CDS             955..2292
                     /gene="adiC"
                     /locus_tag="ACHKNP_RS20460"
                     /old_locus_tag="pgaptmp_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HGU4706159.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDVPISKD"
     gene            2396..4039
                     /gene="eptA"
                     /locus_tag="ACHKNP_RS20465"
                     /old_locus_tag="pgaptmp_004093"
     CDS             2396..4039
                     /gene="eptA"
                     /locus_tag="ACHKNP_RS20465"
                     /old_locus_tag="pgaptmp_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HGU4706160.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4036..4704
                     /gene="basR"
                     /locus_tag="ACHKNP_RS20470"
                     /old_locus_tag="pgaptmp_004094"
     CDS             4036..4704
                     /gene="basR"
                     /locus_tag="ACHKNP_RS20470"
                     /old_locus_tag="pgaptmp_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HGU4706161.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4705..5805
                     /gene="pmrB"
                     /locus_tag="ACHKNP_RS20475"
                     /old_locus_tag="pgaptmp_004095"
     CDS             4705..5805
                     /gene="pmrB"
                     /locus_tag="ACHKNP_RS20475"
                     /old_locus_tag="pgaptmp_004095"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HGU4706162.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5781..5870)
                     /gene="pmrR"
                     /locus_tag="ACHKNP_RS20480"
                     /old_locus_tag="pgaptmp_004096"
     CDS             complement(5781..5870)
                     /gene="pmrR"
                     /locus_tag="ACHKNP_RS20480"
                     /old_locus_tag="pgaptmp_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HGU4706163.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5982..7484)
                     /gene="proP"
                     /locus_tag="ACHKNP_RS20485"
                     /old_locus_tag="pgaptmp_004097"
     CDS             complement(5982..7484)
                     /gene="proP"
                     /locus_tag="ACHKNP_RS20485"
                     /old_locus_tag="pgaptmp_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HGU4706164.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7748..8626)
                     /gene="rdcB"
                     /locus_tag="ACHKNP_RS20490"
                     /old_locus_tag="pgaptmp_004098"
     CDS             complement(7748..8626)
                     /gene="rdcB"
                     /locus_tag="ACHKNP_RS20490"
                     /old_locus_tag="pgaptmp_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HGU4706165.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            complement(8623..10851)
                     /gene="crfC"
                     /locus_tag="ACHKNP_RS20495"
                     /old_locus_tag="pgaptmp_004099"
     CDS             complement(8623..10851)
                     /gene="crfC"
                     /locus_tag="ACHKNP_RS20495"
                     /old_locus_tag="pgaptmp_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HGU4706166.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALASANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQRTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2734 a         2848 c         2704 g         2661 t
ORIGIN      
        1 ggtatcgctt tgctttagcg agaactggtc ttttatttgc actcaggagt acatgtatga
       61 ggatttgcag cgaccaacct tgtattgttt tattgactga aaaagatgtc tggataaggg
      121 tgaatgggaa agaacctatt agccttaaag ctaaccatat ggcgttatta aattgtgaaa
      181 ataatattat cgacgtctcc tctcttaaca acactttggt tgctcatatt agtcacgaca
      241 tcatcaaaga ttacctccgg tttctgaata aagatctctc gcaaatacca gtatggcaac
      301 gtagcgctac gcccatactc accctgccat gcctgacgcc agacgtcttt cgcgttgccg
      361 cgcaacacag catgatgccc gcagaaactg agtcagaaaa ggaacgaaca cgcgcattat
      421 tattcacggt gctatcccgt tttctcgaca gtaaaaaatt cctttcatta atgatgtata
      481 tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat tgaaagcgat attcacaaag
      541 actggaatct tagtatggta gccagttgtt tatgtcttag cccaagtctg ttaaagaaaa
      601 agttgaaaag cgaaaacacc agttatagcc aaataatcac cacctgccgc atgcgttatg
      661 ccgtaaatga attaatgatg gacggtaaaa atatctctca ggtatcacag tcctgcggct
      721 acaacagtac gtcgtacttt atttctgtct ttaaagactt ctacggtatg acgccgctgc
      781 attatgttag tcagcacaga gaacgcactg tcgcctgatt tttaacctta acgaagagct
      841 atattaataa cggcatcagc gataacccgg tcgataataa ttcaactatc gaatgcaggc
      901 gtatgatatg acgtaattta ttgtcacgaa gctcgccttc gcaggagttt aattatgtct
      961 tcggatgctg atgctcacaa agtaggctta atccccgtca ccctgatggt gtcggggaat
     1021 attatggggt caggtgtttt tctgttacct gcaaacctgg cctctactgg cgggattgcc
     1081 atttatggat ggttggtgac gattatcggt gcgctggggc tctcgatggt atacgccaaa
     1141 atgtcgttcc tcgatccaag tcctggtggt tcttacgcgt acgcccgccg ctgctttggc
     1201 ccgtttctcg gttatcaaac caacgtcctc tactggctgg cctgctggat cggcaatatc
     1261 gccatggtgg tcattggcgt aggatattta agttacttct tcccgattct gaaagatcca
     1321 ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct tcgtcctgct gaacattgtc
     1381 ggtccgaaaa tgatcacccg tgtgcaggcc gttgccaccg tgctggcgct gatccccatc
     1441 gtcgggattg ccgtatttgg ctggttctgg ttccgtggtg aaacctatat ggcggcatgg
     1501 aacgtcagcg gcctgggcac cttcggtgca attcaaagta cccttaacgt tacgctgtgg
     1561 tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg tggtgaaaaa cccgaaacgc
     1621 aatgtcccta tcgccaccat tggtggggta ttgattgccg ccgtttgcta tgtactttct
     1681 accaccgcga ttatggggat gatccctaat gccgcactgc gcgtttctgc ttcgccattc
     1741 ggtgatgccg cacggatggc gttgggtgac accgccgggg ccattgtttc cttctgcgca
     1801 gctgcgggtt gcttaggttc actgggcggc tggacgttgc tggcgggtca aacggcgaaa
     1861 gccgctgccg atgacggact gttcccaccg atttttgccc gtgtaaataa agcgggcacg
     1921 ccagtggcgg ggctgattat cgtcggtatt ttgatgacca tcttccagct cagcagcatt
     1981 tcaccaaacg cgaccaaaga gttcggtctg gtttcttccg tctcggtcat ctttacactg
     2041 gtgccatatc tttacacctg tgcggcgtta ctgctgctcg gtcacggtca ctttggtaaa
     2101 gcacgcccgg catatctggc tgttactacc attgccttcc tctactgcat ttgggccgtg
     2161 gtggggtccg gggcgaaaga ggttatgtgg tcatttgtca ccctgatggt aatcaccgcc
     2221 atgtatgcgc tgaattacaa ccggctgcat aaaaacccgt atcccttaga tgtaccaata
     2281 agcaaagatt aattcccctt aatccagcaa acataaaagc caaccttaag aacttaaggt
     2341 tggcttaatt ttgctttgcg agcatatgcg cactttgttc gatggaaaca ccgtgatgtt
     2401 gaagcgccta ctaaaaagac cctctttgaa tttactcgcc tggctattgt tggccgcttt
     2461 ttatatctct atctgcctga atattgcctt ttttaaacag gtgttgcagg cgctgccgct
     2521 ggattcgctg cataacgtac tggttttctt gtcgatgccg gtcgtcgctt tcagcgtgat
     2581 taatattgtc ctgacactaa gctctttctt atggcttaat cgaccactgg cctgcctgtt
     2641 tattctggtt ggcgcggctg cacaatattt cataatgact tacggcatcg tcatcgaccg
     2701 ctcgatgatt gccaatatta ttgataccac tccggcagaa agttatgcgc tgatgacacc
     2761 gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct gcgctgattg cctgctggat
     2821 aaaaatcaaa cctgccacct cgcgtctgcg cagtgttctt ttccgtggag ccaatattct
     2881 ggtttctgta ctactgattt tgctggtcgc cgcactgttt tataaagact acgcctcgtt
     2941 gttccgcaat aacaaagagc tggtgaaatc cttaagcccc tctaacagca ttgttgccag
     3001 ctggtcatgg tactcccatc agcgactggc aaatctaccg ctggtgcgaa ttggtgaaga
     3061 cgcccaccgc aacccgttaa tgcagaacga aaaacgtaaa aatttgacca tcctgattgt
     3121 cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc tacccgcgtg aaactaaccc
     3181 gcggctggcg aaagataacg tggtctattt ccctaatacc gcatcttgcg gcacggcaac
     3241 ggcagtttca gtaccgtgca tgttctcgga tatgccgcgt gagcattaca aagaagagct
     3301 ggcacagcac caggaaggcg tgctggatat cattcagcga gcgggcatca acgtgctgtg
     3361 gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc gtgcctcacc agaacgtcac
     3421 cgcgctgaat ctgcctggtc agtgcatcaa cggcgaatgc tatgacgaag tgctgttcca
     3481 cgggctggaa gagtacatca ataacctgca gggtgatggc gtgattgtct tacacaccat
     3541 cggcagccac ggtccgacct attacaaccg ctatccgccg cagttcagga aatttacccc
     3601 aacctgcgac accaatgaga tccagacctg tagcaaagag caactggtga acacttacga
     3661 caacacgctg gtttacgtcg actatattgt tgataaagcg attaatctgc tgaaagaaca
     3721 tcaggataaa tttaccacca gcctggttta tctttctgac cacggtgaat cgttaggtga
     3781 aaatggcatc tatctgcacg gtctgcctta tgccatcgcc ccggatagcc aaaaacaggt
     3841 gccgatgctg ctgtggctgt cggaggatta tcaaaaacgg tatcaggttg accagaactg
     3901 cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac aatttattct ccacgctatt
     3961 gggattaact ggcgttgaga cgaagtatta ccaggctgcg gatgatattc tgcaaacttg
     4021 caggagagtg agtgaatgaa aattctgatt gttgaagacg atacgctgtt attgcaggga
     4081 ttgattctgg cggcgcaaac tgaaggctac gcgtgcgatg gcgtgacaac cgcgcggatg
     4141 gcggaacaaa gccttgaggc cggtcattac agcctggtgg tactggattt agggttaccc
     4201 gacgaagatg gactgcattt tctcgcccgt atccggcaga aaaaatacac cctgccagta
     4261 ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg ctgggctgga tgtcggtgcc
     4321 gatgactatc tggtgaagcc ttttgcgctg gaagagttac acgcccgtat ccgcgccctg
     4381 ctacgacgcc ataataatca gggcgaaagt gagctgattg ttggcaatct gacgctgaat
     4441 atgggtcgcc gtcaggtatg gatgggcggt gaagagttga ttctgacgcc caaagaatat
     4501 gctctgctgt cacggttaat gctcaaagca ggcagtccgg tgcatcggga aattctctac
     4561 aacgacatct ataactggga caatgaaccc tcgaccaaca ccctggaagt gcatatccac
     4621 aatctgcgcg acaaagtggg caaagcccgt atccgcaccg tgcgcggctt tggctatatg
     4681 ctggtcgcga atgaggaaaa ctaattgaat ctgatgcatt ttctgcgccg accaatatcg
     4741 ctgcgccaac ggctgatatt gaccatcggg gccattttgt tggtgtttga gctgatcagc
     4801 gtcttctggc tatggcatga aagtaccgag cagattcagc tgtttgagca ggcgctacgc
     4861 gacaatcgca acaacgatcg ccacatcatg cgtgagatcc gcgaggcggt cgccagcctg
     4921 attgtccccg gcgtctttat ggtcagcctg acgctattta tctgctatca ggcggtgcgc
     4981 cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg aagcgcgcac tgccgacaac
     5041 ttaacgccca ttgccattca cagcgccacc ctcgaaatcg aagcggtggt ttcggcgtta
     5101 aacgatctgg tcagtcgcct gaccagcacg ctggataacg aaaggttgtt taccgctgac
     5161 gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt tgcatctgga actgctggcg
     5221 aaaacgcatc acattgatgt agcaccgtta gtggcacgac ttgatcagat gatggagagc
     5281 gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat tttcttccgg taattatcaa
     5341 catgtaaaac tgctggaaga tgtcattctc ccctcgtatg acgaactcag taccatgctc
     5401 gaccagcgac agcaaaccct gctattgcca gagagcgccg ccgacatcac cgttcagggt
     5461 gatgcaaccc tgctgcggat gttattgcga aacctggtag aaaacgccca tcgttacagc
     5521 ccgcaaggca gcaacattat gattaagctg caagaagatg gcggagcggt catggcagtt
     5581 gaagatgaag gaccaggtat tgatgagagt aaatgcgggg agttgagtaa agcgtttgta
     5641 cgtatggaca gccgttatgg cgggattggt ctggggttaa gtattgtcag ccgcattaca
     5701 cagttgcatc acgggcagtt tttcctgcaa aaccggcaag agacttccgg cacgcgggcc
     5761 tgggtacggc tgaagaaaga tcagaacgtg gcaaaccaga tataaagaaa gctgctgacc
     5821 accagcacgc tgaacacggt agttaaactt tcataaacac ggtttttcat tgcactctcc
     5881 tccgttaacc tggaggagag tatgcgcgtc agagattaag tgaaccttaa gagttcaatt
     5941 ggcctgataa gacagcgtca catcaggcca tccgtttcag cttattcatc aattcgcgga
     6001 tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg caatctcgtg gtcaatatta
     6061 tcgattttct gctcgatatt atcgtaatgc tcgacgagaa tttccttcgc ttcctgtata
     6121 tctgacgccg ccggtgtcgc acctttcaac ggacgatttg ccgtctcttt catggttacg
     6181 ccggtgatta aaccaatcac cgccactacc atcaggtaat aggcaggcat catcagattc
     6241 tgcgagcttt cgaccagcca ggccgccagt gttggcgtca gaccggcaac cagcaccgaa
     6301 atattaaatg ccgccgccag cgcgctgtaa cggatatgcg tcgggaacat cgctggcaag
     6361 gtagaagcca taacgcccgt aaagcagtta aggatcaccg ccagcatcag taacccggca
     6421 aaaatcaggc cgatgacgtt actgttaatc agaataaacg ccgggatcgc caacacaaac
     6481 agggcaacac taccaagtag cacaaacgga cgacggccaa aacggtcact cagcaagccc
     6541 atcaccggct ggacaaacag cataccgatc ataatggcga taataatcag caccccgtgg
     6601 tcttcggagt aatgcaggtt atgcgacaaa taactcggca tataggtcag caacatgtag
     6661 taagtcacgt tggtggcaat taccagacca atacatgtca acaggctgcg ccagtgtttg
     6721 gtggcaatct ctttaaacga gactttcggg ccatcctgca aaccttcgcg gtcgccctgt
     6781 tccagtttat cgacatgctg ctggaacgcc ggagtttctt ccagcgcatg gcgcaggtaa
     6841 agcccgataa tccctaacgg cagagcaata aagaacggaa tacgccagcc ccagtcgagg
     6901 aagttcgctt cgccgacaat ggtcgaaatt aacaccacta cgccagcacc cagcacaaac
     6961 ccggcaatag aaccgaagtc cagccagctg cccataaagc cacgtttacg gtcaggtgag
     7021 tattccgcaa caaatatcga cgccccggta tattcaccgc cgaccgagaa accttgtgcc
     7081 atcttacaga tcaacagcag aatcggtgcc caaataccaa tcgtgtcgta ggacggtatt
     7141 aagccaatac agaacgtact gatcgacata atcacaatag tgatagcgag gatcttctgg
     7201 cgaccatatt tatcgcccaa cataccaaag aagagcccgc caagcggtcg aatcagaaag
     7261 ggaacggaga aagtggcaag tgcagcaacc atctgcacgc tggggtcagc ccccgggaaa
     7321 aaaactttac ctaatgcgta agcaacaaaa ccataaacac caaaatcaaa ccattccatt
     7381 gcattaccca gtgatgctgc ggtaatggct ttacgcagtt taccgtcatc aataatggtg
     7441 acatcacgaa gggtaattgg ttttactttt ttccttttca gcatagcttt cctcgcagag
     7501 ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg gccgcaaaag gggagcggga
     7561 attgcaggat gcaatctctg taacaagcgt agcgggtttg cttacaccct ccggttaagg
     7621 aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt aatgaccttt ttaccctacc
     7681 agtgtttaaa tttgtgatca acttcacaca tggatttcgt ttaggactca ttgatgtaac
     7741 tgattattta cgccaaattt acagagaaga tttcctcgag cattgccccc gccagacgct
     7801 gcgctgaaca gagtcgtggc atccccagcg cgacagtttg ccagcgttgg gtcactgacc
     7861 agcaaacggg ttgtttatct gccgtggtcc actttcccag ccaacttaaa aaatcagcat
     7921 gatccccgcc cgtcggaacg gtcgaagagg caaaccactg atggtaatag tgacgcgtgg
     7981 tcggggccgc attgatactc tctgccagat gttgtgtcgc tttcacgcgc aaatcaccca
     8041 gcaacaacgc caccgcttgc gggcttccca gacgacaggc atcgccaaat gccccccaac
     8101 gcaactcttc gagaacgata aaacagcgcc caggcaacga ccaggcgtta tacgccccag
     8161 cacgccagcg ggtaaaaatt tgctcgctct gatcacctgc ctgcaccagt aggcctcgtt
     8221 gcgttaacgc cttttccttc cagcggcaac gggctgaaaa ccactcatac aaggcggcaa
     8281 tttgctgggg gagctccggg aaagcagagt gctggcggtg atccattttt tgcagcagcg
     8341 ccagatgctg cgcgatctgc gtcagtgcca gatgaccggg atccagcatc gacgccagtt
     8401 tttccaccaa tgtcagacgc aggctaagcg gcgcatttaa catgccgccg ttcgtccatg
     8461 tctgaagctg cacgcgcgca agaagttcct gcatcagtcg ttcacgaaac tgctgctgat
     8521 gggcttgtgg aaggcgggca tcatcgccct gcgccagatc cactaaaaag cggggataaa
     8581 ccgactccag cacgcgaccg gggccgtcaa gtaacgtctt ggtcaatatc gttctgccgt
     8641 gaaaagtgtt tgaatatcat cgcgtaacag ctgggcgtcg gtgtaaatcc agccgtgagt
     8701 catcacagtc cgctgcaatt gctgctgcat cagcctgacc accgattcat tttgttgacg
     8761 cagagccaga ctttcgcgta aacgcgtctg taattccgtc aaacatgaag cgaactcagc
     8821 gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc tgctctgcca gaactttagc
     8881 catttgttgg caataaagat cgacttctgc gcttaaagct cgttgcaaca cactgtaatc
     8941 aaccgtctct gtcggggatt tctcatttcc ccgtccccag tcgggctgat tcaaccagcg
     9001 cgaaaaagtc tcacgcacaa cgcctaaacg cgtgctctgc tcgtccgttg cttcctggcg
     9061 cgaaatgact tcactgaaca gctggcgagt gttgaagtgg ggaactgcgc cgtgaaaaac
     9121 aggaaaatga aacccaggac gaaaccctga ctcgctcaat tccattttga cttgttgctc
     9181 aatggggcga ataacatcgg ttaacacccg gcaaagggtg gattccagct cggcaaaacg
     9241 cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt aacagtgtct cacagcgggt
     9301 acgaatctcg cttaacggct ccgaatcatc ctgaaacaac gcggctaact gtgcattcac
     9361 cgcatcttgc tgttgacgca gaaagtggtt ggcggaggcc aaggccagct cgatttcatg
     9421 tttaatctcg ccgctcacct gcgcctgatt gagttgcaat agctgcaaac tttcttcgac
     9481 ctgatggata ttttgccgca attgttcaca agcgacgttt aacccgtgcg cacgaaaatc
     9541 caggtattcc cgcgcctgct gcgcgtaatt caacagttta tgcgcggcag atcgcagagc
     9601 atacaacgag gcgttagcgt aagcggcatg aagcaacgcc tgaattggct gggcgaacag
     9661 cgaatcttcc cacaactgat cggcagcatg acgaatatgt tcgaggtccg ccagatcggc
     9721 atgacgccag cgcctgccga gcgcggcatg ggcaaaatct tccacccagc gttgttgctc
     9781 tggcggtggt aacttaccgt tgttggctaa ctcatggcgc gcccgattcg ccaggtagcc
     9841 ccacatcgac gacaccggaa atatctgctg tggcgtaata cagcctttca tcagcgtccc
     9901 ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta cgatcctgtt gatcgaactt
     9961 attgaccagc acatacagcg gcaccgattg ccccaccgcc aaaatcgcct cacggacctc
    10021 ttcatcggag atcgatttca gttgcgtata atccagcacc gccagtaccg ccgaggcgcg
    10081 tgccagctgc tggttaagca ttttttgcag atgcggttgc ccggcttcat ttggcccggg
    10141 ggtatccagt aacgtcaact gaccgggata actttccagc cccgccagat ggacaaactc
    10201 cacttcaatc acgggaatat gctcaatggc ggcgtaagca gaaaaaggaa aatcgacgtc
    10261 cagcgccttc gccagtcgca ctaaatcatt caaacttttc agacaatgaa aaataggctg
    10321 ggcacccaga taatattttt cgaaagcgac gccattttcg atccgctgca taagcgcacg
    10381 catatcttta tctatttcca gcacatcggt cagatgctta atatcgcaat cacgcaggcg
    10441 ctgttgtaat ttttgaatta aacggtcgat tggcgcgaca tgtgaaaaat gcagtaccgg
    10501 ttctttttgc ccgggcgtat ggcgaataag cgtcggcagc gcagtcattg ggcgattacg
    10561 attaggcaaa acctccgtac caacaatggc attaatggtg gttgatttcc ctgctttcat
    10621 ggtaccgaca atcgccagca ccatttccag tcgggaaatt ttacgcaact cattattcag
    10681 catcgcgtga cgttcggcga tattaggctg actccagggt aaagccagtt gtggcgcatc
    10741 gtctccgggt acagagagag gcattttttc cagtaactgc aactgttggc gagaaagctg
    10801 taacaggcgt tcagcctcct gacttaactc atacagggtc tgtgtgtaca tagaaaattc
    10861 ttccttaaag caaattttgt tacttttatt tagccagatt gtttttgagt tctgttttcg
    10921 gcttttataa ttactgcaag aaataat
//
LOCUS       DAZFWL020000145        10898 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFWL020000145 DAZFWL020000000
VERSION     DAZFWL020000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.145"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            31..942
                     /gene="hlyE"
                     /locus_tag="ACHKNP_RS20500"
                     /old_locus_tag="pgaptmp_004100"
     CDS             31..942
                     /gene="hlyE"
                     /locus_tag="ACHKNP_RS20500"
                     /old_locus_tag="pgaptmp_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HGU4706167.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QNFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(1149..1595)
                     /gene="ycgN"
                     /locus_tag="ACHKNP_RS20505"
                     /old_locus_tag="pgaptmp_004101"
     CDS             complement(1149..1595)
                     /gene="ycgN"
                     /locus_tag="ACHKNP_RS20505"
                     /old_locus_tag="pgaptmp_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HGU4706168.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1687..2346)
                     /gene="ycgM"
                     /locus_tag="ACHKNP_RS20510"
                     /old_locus_tag="pgaptmp_004102"
     CDS             complement(1687..2346)
                     /gene="ycgM"
                     /locus_tag="ACHKNP_RS20510"
                     /old_locus_tag="pgaptmp_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGU4706169.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(2418..2711)
                     /locus_tag="ACHKNP_RS20515"
                     /old_locus_tag="pgaptmp_004103"
     CDS             complement(2418..2711)
                     /locus_tag="ACHKNP_RS20515"
                     /old_locus_tag="pgaptmp_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HGU4706170.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(2723..2881)
                     /locus_tag="ACHKNP_RS20520"
                     /old_locus_tag="pgaptmp_004104"
     CDS             complement(2723..2881)
                     /locus_tag="ACHKNP_RS20520"
                     /old_locus_tag="pgaptmp_004104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706171.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            2952..3353
                     /gene="pliG"
                     /locus_tag="ACHKNP_RS20525"
                     /old_locus_tag="pgaptmp_004105"
     CDS             2952..3353
                     /gene="pliG"
                     /locus_tag="ACHKNP_RS20525"
                     /old_locus_tag="pgaptmp_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HGU4706172.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3456..3824)
                     /gene="ycgJ"
                     /locus_tag="ACHKNP_RS20530"
                     /old_locus_tag="pgaptmp_004106"
     CDS             complement(3456..3824)
                     /gene="ycgJ"
                     /locus_tag="ACHKNP_RS20530"
                     /old_locus_tag="pgaptmp_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HGU4706173.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDASGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            4344..5039
                     /gene="minC"
                     /locus_tag="ACHKNP_RS20535"
                     /old_locus_tag="pgaptmp_004107"
     CDS             4344..5039
                     /gene="minC"
                     /locus_tag="ACHKNP_RS20535"
                     /old_locus_tag="pgaptmp_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HGU4706174.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5063..5875
                     /gene="minD"
                     /locus_tag="ACHKNP_RS20540"
                     /old_locus_tag="pgaptmp_004108"
     CDS             5063..5875
                     /gene="minD"
                     /locus_tag="ACHKNP_RS20540"
                     /old_locus_tag="pgaptmp_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HGU4706175.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5879..6145
                     /gene="minE"
                     /locus_tag="ACHKNP_RS20545"
                     /old_locus_tag="pgaptmp_004109"
     CDS             5879..6145
                     /gene="minE"
                     /locus_tag="ACHKNP_RS20545"
                     /old_locus_tag="pgaptmp_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HGU4706176.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6517..6861)
                     /locus_tag="ACHKNP_RS20550"
                     /old_locus_tag="pgaptmp_004110"
                     /pseudo
     CDS             complement(6517..6861)
                     /locus_tag="ACHKNP_RS20550"
                     /old_locus_tag="pgaptmp_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6896..7015)
                     /locus_tag="ACHKNP_RS20555"
                     /old_locus_tag="pgaptmp_004111"
     CDS             complement(6896..7015)
                     /locus_tag="ACHKNP_RS20555"
                     /old_locus_tag="pgaptmp_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGU4706177.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6976..7161)
                     /gene="ymgJ"
                     /locus_tag="ACHKNP_RS20560"
                     /old_locus_tag="pgaptmp_004112"
     CDS             complement(6976..7161)
                     /gene="ymgJ"
                     /locus_tag="ACHKNP_RS20560"
                     /old_locus_tag="pgaptmp_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HGU4706178.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7262..7435
                     /gene="ymgI"
                     /locus_tag="ACHKNP_RS20565"
                     /old_locus_tag="pgaptmp_004113"
     CDS             7262..7435
                     /gene="ymgI"
                     /locus_tag="ACHKNP_RS20565"
                     /old_locus_tag="pgaptmp_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HGU4706179.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7437..7781
                     /gene="ymgG"
                     /locus_tag="ACHKNP_RS20570"
                     /old_locus_tag="pgaptmp_004114"
     CDS             7437..7781
                     /gene="ymgG"
                     /locus_tag="ACHKNP_RS20570"
                     /old_locus_tag="pgaptmp_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HGU4706180.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7785..8120
                     /gene="ymgD"
                     /locus_tag="ACHKNP_RS20575"
                     /old_locus_tag="pgaptmp_004115"
     CDS             7785..8120
                     /gene="ymgD"
                     /locus_tag="ACHKNP_RS20575"
                     /old_locus_tag="pgaptmp_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HGU4706181.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8177..10498)
                     /locus_tag="ACHKNP_RS20580"
                     /old_locus_tag="pgaptmp_004116"
     CDS             complement(8177..10498)
                     /locus_tag="ACHKNP_RS20580"
                     /old_locus_tag="pgaptmp_004116"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4706182.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     GSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(10615..10824)
                     /locus_tag="ACHKNP_RS20585"
                     /old_locus_tag="pgaptmp_004117"
     CDS             complement(10615..10824)
                     /locus_tag="ACHKNP_RS20585"
                     /old_locus_tag="pgaptmp_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706183.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
BASE COUNT         3030 a         2594 c         2384 g         2890 t
ORIGIN      
        1 caatttatat atttaaagag gcgaatgatt atgactgaaa tcgttgcaga taaaacggta
       61 gaagtagtta aaaacgcaat cgaaaccgca gatggagcat tagatcttta taataaatat
      121 ctcgatcagg tcatcccctg gcagaccttt gatgaaacca taaaagagtt aagtcgcttt
      181 aaacaggagt attcacaggc agcctccgtt ttagtcggcg atattaaaac cttacttatg
      241 gatagccagg ataagtattt tgaagcaacc caaacagtgt atgaatggtg tggtgttgcg
      301 acgcaattgc tcgcagcgta tattttgcta tttgatgagt acaatgagaa gaaagcatcc
      361 gcccagaaag acattctcat taaggtactg gatgacggca tcacgaagct gaatgaagcg
      421 caaaaatccc tgctggtaag ctcacaaagt ttcaacaacg cttccgggaa actgctggcg
      481 ttagatagcc agttaaccaa tgatttttca gaaaaaagca gctatttcca gtcacaggta
      541 gataaaatca ggaaggaagc gtatgccggt gccgcagccg gtgtcgtcgc cggtccattt
      601 ggattaatca tttcctattc tattgctgcg ggcgtagttg aagggaaact gattccagaa
      661 ttgaagaaca agttaaaatc tgtgcagaat ttctttacca ccctgtctaa cacggttaaa
      721 caagcgaata aagatatcga tgccgccaaa ttgaaattaa ccaccgaaat agccgccatc
      781 ggggagataa aaacggaaac tgaaacaacc agattctatg ttgattatga tgatttaatg
      841 ctttctttgc taaaagaagc ggcaaaaaaa atgattaaca cctgtaatga gtatcagaaa
      901 agacacggta aaaagacact ctttgaggta cctgaagtct gataagcgat tattctctcc
      961 atgtactcaa ggtataaggt ttatcacatt taaaaacaca gacgtttttt aaagattgag
     1021 agttacctgt tgataataag gtatcgggta actctcatta tgacagagcg ctttcaaaaa
     1081 cagggctacg aaatggcgag catccagacg tatattaaga aagcccccat caacatctat
     1141 taaatttatc actgcgccca gtcaggttta tttaagatat gatcctgcca gtcaatcact
     1201 tctgattctt tcactgcgat atgacgcaca gagatacgtt caccatgcat tgccgctttc
     1261 gaacccgtaa gtagcggatg ccacgcaggt aaatctttac cttccgccag caaccgataa
     1321 gcgcaggtca ttggcagcca ttcgaatgtt ggcagatttt cacgggttaa tttaatgcag
     1381 tcgggttcaa actcgaaacg acgttcgtag ttccgacatt gacaggtttt aatattgagc
     1441 tggcgacagg cgacgttagt gaagtagatt tcgtcggtgt cttcatccat cagtttatgc
     1501 aggcaacact gaccgcaacc atcacacaac gactcccatt ccgcatcgct catttcgtcc
     1561 agggttttac tttgccagaa aggtacatcg ctcatcaggt gttccgccat tacgttaaaa
     1621 cgcaccttat aaccagtctg gcacagcgat gcaagttttg ccgccgcttt caggcggcaa
     1681 aaagtattac aaaacgcgag ttgccaaaga atgcccatcg aaagtgactg tcagctcatc
     1741 accgctttgc aacgggccga cgccatcagg cgtgcctgtc agcacaacgt caccggcctt
     1801 gagggtaaaa aacttgctca tataagcgat cagcggaaca attttatgga tcatgtccgc
     1861 agtcgtacct tgctgacgtt gctcgccgtt tacgctcagg cccagcgttg tattttgcgg
     1921 gtcgccggta aattccgccg cgggaataaa cccggaaagc ggacaagagt tatcaaacgc
     1981 tttagccttt tcccacggct gcccggcttt cttcattttt ccctgaacat cacgcaatgt
     2041 cagatcgagc gccacgccat aaccggcaat ggctttgcgg acatgctctt ccgtagcctg
     2101 acgcagcgtc gcgccaatca acaccgccag ttcgacttca tgatgaactg aaccgaaatc
     2161 ggatgggatc gccaatggct gccgcagatc gcacagtgcc gtttctggtt taataaacag
     2221 cactggctct tcgggcactg cgctgcccat ctctttaata tgtttggcat agttactgcc
     2281 aacacagact actttactca ccggataatc cagcagcgca ccttgccagt tgtgatgttg
     2341 atacataacg ttcccctcac cttatcgtgg atatgaaaac ccgcgccgac aaagataatg
     2401 ccaccgccgg atatcggtta tttgttagtg tcgtctgttt tctgccccat gacggaaaga
     2461 tgttgcttca gcaaatcttc gggtggtggc ggtaactgca aatagtaacc ttgctcggtt
     2521 aatgccagtt taaccttttc aatatcagca ttgaccagtt tcttccgccc atccaacggc
     2581 agaatcatcg ctaactgagg ctgaccaaaa cctttcatca gttcctcagg aacacgcgaa
     2641 aaatcgtctt ttttttcgac atataaatag gtctggtcac gcttgctgct tcgataaatc
     2701 acacaaaaca tacttttact ctttaagatg ccaggttttg gcataagcta acatcaatga
     2761 aacccgcgcc acaagccagc cagctgtaga tttctacggt ttgttgagtc catgcccctt
     2821 tataagtaat ccccccggca aaaatgccgc gccgggattt taccgaacgc aggaaagcca
     2881 ctggaattct cccgttaggg attgttatat tataacagtt catcgtactc attctgaaca
     2941 ggagactacc aatgaaaatc aagagcatca gtaaggctgt attgctgttt gcactgctaa
     3001 cctccacgag ctttgctgcg ggtaagaatg tcaatgttga gttcagaaaa ggccatagca
     3061 gcgctcagta ctccggcgaa ataaagggat acgattacga tacatatacc ttctatgcca
     3121 aaaaaggtca gaaagtgcat gtgagtatct ctaatgaagg cgctgatact tacctcttcg
     3181 ggccaggaat cgatgattca gttgatttat ccagatattc cccagaactc gatagtcacg
     3241 gacaatactc actccctgct tctggcaaat atgagctgcg agtgttgcaa acccgaaacg
     3301 acgcccgtaa aaacaaaact aaaaaatata acgtcgatat tcaaattaag taaatgtcaa
     3361 ccgacgggga ctctccccgt cataaacgtt aacgccccac tcctctctgt cgctcaagag
     3421 gttaatgacg aatagaacat aatgtaaaca agacatcact cacgacacat aaataacatc
     3481 tttgtggtgg tttgattgat ttttccactc cgcttaccat ctacaccaaa ataacgatca
     3541 tcacgacata atttttcttt aacatcgcaa aatacaccgt tagcgaatgt aaattcgctg
     3601 ggatcgtagc cttgtaatga ttgtaagttt tcagcggctt tttcgccgag atatttcctt
     3661 gttaactctg gggaaatgcc atctgagttc acacaaatat acgggtcgca aacaacgccg
     3721 cacgccggag attgaagtcc gctagcgtca tgtccagacg ccattgatga gaacatcatc
     3781 cccacacctg acaatccgat ataaataata cgcatcatat tcatagcaca tcctgataat
     3841 ccaaccgtac aacacttatg ctcatttcgt catcaatccg taatcaaaac cgctgacctg
     3901 gacgagttac gatgacctga cagaagtatc agacgtcttc tgcggtttct atgatagagc
     3961 aattctgtgg cggatattaa gataacgggt atctgttctt taatgatgcc gcaaaaaatg
     4021 gaaagttaag aaagcctggc gtggtgaaaa aatacacttg cctcaatact ggatatttaa
     4081 acaggtctgg agatgctgtc acgattaggg tgcggaaaaa ggaagcgtaa ataagaagaa
     4141 tcaaaagtac gacggcaatg ggttgattga caagggtatt ttttaagcta tgaatcagcg
     4201 ccatttatca cagaatagac ttttactctg aataaatggg agggtgactt gcctcaatat
     4261 aatccagact ataacatgcc ttatagtctt cggaacatca tcgcgcgctg gcgatgatta
     4321 atagctaatt gagtaaggcc aggatgtcaa acacgccaat cgagcttaaa ggcagtagct
     4381 tcactttatc tgtggttcat ctgcatgagg cagaacctaa ggttatccat caggcgctgg
     4441 aagacaaaat cgctcaggcc cccgcatttt taaaacatgc ccccgttgta ctcaacgtca
     4501 gtgcactgga agacccggta aactggtcag cgatgcataa ggcggtttcg gcaaccggtt
     4561 tgcgggttat tggcgtaagt ggctgcaaag atgcgcaact taaagccgaa attgaaaaaa
     4621 tggggctgcc tatcctgacg gaaggaaagg aaaaagcgcc acgtccagct cccgcaccgc
     4681 aggctccagc gcaaaataca acgccggtca caaaaacgcg tttaatagat accccggtgc
     4741 gttccggtca gcgtatttat gctccacaat gtgatctgat tgttacaagc cacgttagcg
     4801 ctggggccga attgattgcc gatggtaaca ttcatgtcta tggcatgatg cgcggtcgtg
     4861 cgctggcagg ggcaagtggt gaccgggaaa cgcaaatatt ttgtacgaac ctgatggcgg
     4921 aactggtgtc catcgcaggt gaatactggc tgagtgatca aatcccagca gaattttatg
     4981 gcaaagcggc gcgactgcag ttagtcgaaa acgctttgac cgttcaaccg ttaaattgat
     5041 ccctttttaa caaggaattt ctatggcacg cattattgtt gttacttcgg gcaaaggggg
     5101 tgttggtaag acaacctcca gcgcggccat cgccactggt ttggcccaga agggaaagaa
     5161 aactgtcgtg atagattttg atatcggcct gcgtaatctc gacctgatta tgggttgtga
     5221 acgccgggtc gtttacgatt tcgtcaacgt cattcagggc gatgcaacgc taaatcaggc
     5281 gttaattaaa gataagcgta ctgaaaatct ctatattctt ccggcatcgc aaacacgcga
     5341 taaagatgcc ctcacccgtg aaggggtcgc caaagttctt gatgatctga aagcgatgga
     5401 ttttgaattt atcgtttgtg actccccggc agggattgaa accggtgcgt taatggcact
     5461 ctattttgca gacgaagcca ttattaccac caacccggaa gtctcctcag tacgcgactc
     5521 tgaccgtatt ttaggcattc tggcgtcgaa atcacgccgc gcagaaaatg gcgaagagcc
     5581 tattaaagag cacctgctgt taacgcgcta taacccaggc cgcgtaagca gaggtgacat
     5641 gctgagcatg gaagatgtgc tggagatcct gcgcatcaaa ctcgtcggcg tgatcccaga
     5701 ggatcaatca gtattgcgcg cctctaacca gggtgaaccg gtcattctcg acattaacgc
     5761 cgatgcgggt aaagcctacg cagataccgt agaacgtctg ttgggagaag aacgtccttt
     5821 ccgcttcatt gaagaagaga agaaaggctt cctcaaacgc ttgttcggag gataagttat
     5881 ggcattactc gatttctttc tctcgcggaa gaaaaacaca gccaacattg caaaagaacg
     5941 gctgcagatt attgttgctg aacgccgtcg cagcgatgca gaaccgcatt atctgccgca
     6001 gttgcgtaaa gatattcttg aggtcatttg taaatacgta caaattgatc ctgagatggt
     6061 aaccgtacag cttgagcaaa aagatggcga tatttctatt cttgagctga acgtgacctt
     6121 accggaagca gaagagctga aataagcccg ctgtaaaaac gcatttatct tcaaggcaga
     6181 gtcatttctg ccttgagttt ttcatccctc tcatccacgt tgtggtaaag cggcgagtat
     6241 tcttgctgat actcctcatt gctatttcca cctccccctt tacaccttaa ggctgttccc
     6301 acgcacagaa caaaatgttt atgcctggtt gagtaaataa cctcattgtt agtgtggata
     6361 cactggggga tgataacccc gcaatttcat tattcattgt tctcccatag gatgaggttt
     6421 cccgccgtaa ctggcgggct ttttttgccc cagaattttc ccttttagcc ccctgtaaac
     6481 gaaaactgcg ccgaagcgca gtttaatcgt tgatggtcag aacgaccagt tcacaccagc
     6541 caccgcattc cacggggatt ccacaccggc accatggcta taccccaccc cgaaatgccc
     6601 gcttaacgta ctgctgaatg aggctttaat acctgcctgg tatattccac gtctgcccga
     6661 caaatcattg acaaaattac cgtcactatt cactttcacc cgattatcat cgacaaattc
     6721 tttgctcaca gccgccttca ggcagggttc aatttccata ccgttcccca gacgcatgtt
     6781 gtaactcagc gttgcaccca gttcacgata tatactgcgg gtatcgactg atttcgattc
     6841 catgccattg gataaatgat agtagcgtct aagtgaccta agctctgctg caacatcagc
     6901 ccgcaggcac cagaccaggg gattcatcct gaagagacag cgcaggtgta ttgtgttcac
     6961 cgctcaccaa agacatcatg atgaaatgat gatatcccgc ataagaaaga ggcatttttt
     7021 acacgcagtg cgctgaagtg tagttggata aaaaatcact cttttcagat aatactgtcg
     7081 tacgcttatc tttcgacaat gaggccgtcg gcaaaataaa atgactaatg taatcgttgc
     7141 tgatgaatat cttctgctca cataaaaatc acacaataat attgataaat cagattatct
     7201 tacctttaca acattgcgtt aacagtcaat gacacttctt gaataaaaat attgtcaatc
     7261 aatgagttat tcctcattta agattattct cattcacgta aaaaacataa ttcctatcat
     7321 cacagccacg ctcattttga attatctgaa taatagcgag cgttctttag ttaagcagat
     7381 cttaattgaa gacgaaatta ttgtgtgtgc aacttactta ataccggaca tctaagttga
     7441 agaaaaaaat acttgccttc gggcttatct cagcattgtt ctgctccacg ccagcgatgg
     7501 cggatatgaa tcgcaccacg aaaggtgcat tgctaggcgc aggcgttggt ttacttactg
     7561 gcaatggtgt taatggcgta ctgaaaggtg cagctgttgg cgctggtgtt ggtgcagtaa
     7621 cagaaaaagg ccgcgacggt aaaaatgcgc gtaaaggtgc aaaagtaggt gccgccgttg
     7681 gtgctgtcac tggcgttctg accggcaatg gcctcgaagg ggcgattaaa ggtgccgtga
     7741 ttggcggtac gggtggtgca attctgggta aaatgaaata aggaatgatg atgaaaaaat
     7801 ttgctttact ggcgggcctg tttgttttcg caccaatgac ctgggcgcag gactacaata
     7861 ttaaaaacgg tttgccgtca gaaacctata ttacttgcgc agaagccaac gaaatggcaa
     7921 aaactgacag cgcacaggtt gcagaaattg ttgcggtaat gggtaatgcc agcgttgcca
     7981 gccgtgattt aaaaattgag caatctccgg aactgagcgc caaagtggta gaaaaactga
     8041 atcaggtttg cgctaaagac ccgcaaatgc tgttgatcac ggcaattgat gacaccatgc
     8101 gtgctatcgg caaaaaataa tatttcatat gtaccgcgtc gatgaatacg acgcggtttt
     8161 ttagctaacg gcgggatcag aacttatatt tcaggcccac catcactgcg gtgtcattgt
     8221 agccattatc acccagctgc acgcccacat tcccccagac gctggccgct ggattcaggt
     8281 tgccatttac gccaagttta atttccccca aattactcgc cccatcttgt tcgactttca
     8341 caccgttcat tgagacagca aaatctttac tgttatggat ccagttcatt tcaacaaaag
     8401 gttcaaagtc gtcaccgtgc ggaccgctgt ttttctcctg agtacgaata aaggtgcgga
     8461 aaccaagtcg ggtctggatg ttgttatttg cgtcattctc aatacgtgag ccattggctt
     8521 cggtgtgcgc attatgatcg acgcccatcc agatagcctg tgcctgtggc tggaatatcc
     8581 acgtgtattt cgccgcttcc agaccaaagg atttattcag ggcaaagccg taacccgctt
     8641 ccagcgagac ggttgcgcct ttcgcactat aggattcata ggctaattca tcccctttca
     8701 ccgagttttt aaaccagcta tattgcgccc aggcgtcaat gtatgcgcct tttttactga
     8761 tgtcatcggc aaaccaagtg gcatacagcc ctgcgcaata gcctctgaca ctccctttcg
     8821 aacgataatc cgacacgctg gaatgagttg agttgtagtc gcgggcataa ccagccatca
     8881 ctcccaaacg ccaactgtca ctgtcggtaa aaccaccggt taacagctcg gcccctaact
     8941 gcgagacgta gcgatgcgag gtcgttctca actgtccgtt gctgtcacgc caggcattat
     9001 gcccgccaat ttgacgtagc caaagtcggc ttgaacgctc ctgttcagtt acaggatcga
     9061 tgtagcgcat ttcacccgca cggtcgttca gacgcataac aaaaagagtg ttagcggctg
     9121 ccaggttggc gacgtaactt ccagcttcga ggcgcaggac gggggtaggc gcaacagtag
     9181 gcgctgaact tcctccggag tttgtttcac cgccgctcgt tgcctggaag ttagtcagat
     9241 accagttttt attggtcgct tcggtattac ctcgggttaa tgaatattcg taggcaccgg
     9301 cgaaaatcct cgaatcctta ataaattccc catttgattc cccctcaacg ctgataatct
     9361 ctatcccatt taacgtgtaa gctccgctgc ctcctgcatt ggtaacttta actcgggttg
     9421 ttccagaggt atttccatta ataacgactt tatccgtagc tgaattatca tcacccaatt
     9481 cagttctgag atgcaatacg ccattattac caacgtaatt ttcagttatt gttaatcttg
     9541 ttggttcgac atcccgtcca tctgcccggg aaatatagac cgtactatta tcaaccgtga
     9601 ggttgctcac cgttgagtca gctttaatat cccaggtact attatctgaa agcgacagat
     9661 aggtatgagt gttaacatct gttgaaatat tagctgaacc cgttattttt gaattagttg
     9721 catcgacggt caaatgacct ttaatattat tgataaaaac atctgcagcc gatgatacct
     9781 cactatccgt aatggtcacg ttactcgtct catttttatg gtagaggatt tcatttgctg
     9841 accctagtga gctaccagat aaaatatcta ccgttgcttc ctgaccattt atggaagact
     9901 ccagcacaat tccagacccc tgatgcgcga ctgcagatgt atttttcagc gtcaccgtgc
     9961 cgccattata aattttagca gtcgcggcgt caggacccgt tgtataaatt tctccctggt
    10021 taaatgaaac tgtcccctgg cgggcagcga tggcatgagc attgtcccca tgcgtggtga
    10081 tatcaacatc ggttaatgta acgctggcat tggtataatc aagcgtgtca ccagccttga
    10141 cgacagtgcc attagcgtac gcggcataac tttctgttcc gtgggttgtg atctcccctt
    10201 ttgccatatc cagttttgct atggcattat tttcattact tcctgtatcg gtagtacttt
    10261 cttcatcaag agaagataca cacgcacgcg cgcttccggg agatgcacac gcaagaacgc
    10321 cgactgcatg attacccgca gtaacaataa atgtatcatc tccgtcagaa aaagaggggg
    10381 taaccgaacc gtcagagttg actcttatta tggtgtcagt attcaggttt ccactcgttt
    10441 gacttagcaa accaattgag taatcccccg ttatgacaat cgtgttttta ccctgcaaaa
    10501 taacagcacc gccaaattca cttacgattg cgctagctgt taatgctgtg cttagaggac
    10561 tattgtcaga aggatataaa ccgcccgttg ttacaatgac gccattgtta atggttaact
    10621 tgctaccctc tcctgcaacg gatacccgta acttccatta cccgttgtta taactagcgc
    10681 attatcaata tcttttactt ttccatcttt tattgccaca gcatgtttat attcatcgtg
    10741 ctgaataaac tgttcagcat atgcattacc caccgccaga gcaataagtc ccaaactaaa
    10801 cccggagagt tttttcagtt tcatagatta accatccatt cattggatta acaaacattc
    10861 ctggtcaaat aacctatgcg ttcagattac caaaacgg
//
LOCUS       DAZFWL020000146        10825 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFWL020000146 DAZFWL020000000
VERSION     DAZFWL020000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.146"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            66..1175
                     /gene="anmK"
                     /locus_tag="ACHKNP_RS20590"
                     /old_locus_tag="pgaptmp_004118"
     CDS             66..1175
                     /gene="anmK"
                     /locus_tag="ACHKNP_RS20590"
                     /old_locus_tag="pgaptmp_004118"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HGU4706184.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVEPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            1273..1602
                     /gene="mliC"
                     /locus_tag="ACHKNP_RS20595"
                     /old_locus_tag="pgaptmp_004119"
     CDS             1273..1602
                     /gene="mliC"
                     /locus_tag="ACHKNP_RS20595"
                     /old_locus_tag="pgaptmp_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HGU4706185.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            1661..2317
                     /gene="pdxH"
                     /locus_tag="ACHKNP_RS20600"
                     /old_locus_tag="pgaptmp_004120"
     CDS             1661..2317
                     /gene="pdxH"
                     /locus_tag="ACHKNP_RS20600"
                     /old_locus_tag="pgaptmp_004120"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HGU4706186.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            2446..3720
                     /gene="tyrS"
                     /locus_tag="ACHKNP_RS20605"
                     /old_locus_tag="pgaptmp_004121"
     CDS             2446..3720
                     /gene="tyrS"
                     /locus_tag="ACHKNP_RS20605"
                     /old_locus_tag="pgaptmp_004121"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGU4706187.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            3779..4642
                     /gene="pdxY"
                     /locus_tag="ACHKNP_RS20610"
                     /old_locus_tag="pgaptmp_004122"
     CDS             3779..4642
                     /gene="pdxY"
                     /locus_tag="ACHKNP_RS20610"
                     /old_locus_tag="pgaptmp_004122"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HGU4706188.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(4686..5291)
                     /gene="gstA"
                     /locus_tag="ACHKNP_RS20615"
                     /old_locus_tag="pgaptmp_004123"
     CDS             complement(4686..5291)
                     /gene="gstA"
                     /locus_tag="ACHKNP_RS20615"
                     /old_locus_tag="pgaptmp_004123"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HGU4706189.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(5397..6899)
                     /gene="dtpA"
                     /locus_tag="ACHKNP_RS20620"
                     /old_locus_tag="pgaptmp_004124"
     CDS             complement(5397..6899)
                     /gene="dtpA"
                     /locus_tag="ACHKNP_RS20620"
                     /old_locus_tag="pgaptmp_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HGU4706190.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(7510..8145)
                     /gene="nth"
                     /locus_tag="ACHKNP_RS20625"
                     /old_locus_tag="pgaptmp_004125"
     CDS             complement(7510..8145)
                     /gene="nth"
                     /locus_tag="ACHKNP_RS20625"
                     /old_locus_tag="pgaptmp_004125"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGU4706191.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     LVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(8145..8840)
                     /gene="rsxE"
                     /locus_tag="ACHKNP_RS20630"
                     /old_locus_tag="pgaptmp_004126"
     CDS             complement(8145..8840)
                     /gene="rsxE"
                     /locus_tag="ACHKNP_RS20630"
                     /old_locus_tag="pgaptmp_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HGU4706192.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(8844..9464)
                     /gene="rsxG"
                     /locus_tag="ACHKNP_RS20635"
                     /old_locus_tag="pgaptmp_004127"
     CDS             complement(8844..9464)
                     /gene="rsxG"
                     /locus_tag="ACHKNP_RS20635"
                     /old_locus_tag="pgaptmp_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HGU4706193.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(9468..10526)
                     /gene="rsxD"
                     /locus_tag="ACHKNP_RS20640"
                     /old_locus_tag="pgaptmp_004128"
     CDS             complement(9468..10526)
                     /gene="rsxD"
                     /locus_tag="ACHKNP_RS20640"
                     /old_locus_tag="pgaptmp_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HGU4706194.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(10527..>10825)
                     /locus_tag="ACHKNP_RS20645"
                     /old_locus_tag="pgaptmp_004129"
     CDS             complement(10527..>10825)
                     /locus_tag="ACHKNP_RS20645"
                     /old_locus_tag="pgaptmp_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706195.1"
                     /translation="EPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAA
                     VEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         2710 a         2713 c         2793 g         2609 t
ORIGIN      
        1 catggtattc acgaaaagtt tatgtttgag tggtgcgcaa acgtcagatt gaaaaaggat
       61 gaactatgaa atcgggccgc tttattggcg ttatgtcagg caccagcctt gatggtgttg
      121 atgttgtgtt ggcgacaata gatgaacacc gggtcgcaca gctggcaagc ttgagctggc
      181 cgatcccggt gtctctgaaa caggctgtac tggatatttg ccagggccag cagcttacac
      241 tttcgcagtt tggacagctt gatactcaac tcgggcgact ttttgctgat gcggtcaatg
      301 ccttgcttaa ggaacaaaac ctgcaggcga gagatatagt tgcgatcggc tgtcacggtc
      361 aaaccgtctg gcatgaaccg acgggcgtgg aaccacacac tttacagatt ggcgataaca
      421 atcaaattgt ggcacgcacc ggaattacgg ttgtcggtga ttttcgccgt cgcgatattg
      481 ccttgggagg acaaggcgca ccgctggtac ctgcgttcca tcatgccttg ctggctcacc
      541 caaccgagcg acgaatggtg ctcaatattg gcggcatcgc caatctgtca ctgctcattc
      601 ctgggcagcc ggttggcggc tacgataccg gtcctggtaa catgctgatg gatgcctgga
      661 tctggcgtca ggccggtaaa ccttacgata aagatgccga gtgggcacgg gcgggtaaag
      721 ttattctccc actgctgcaa aatatgctca gcgacccata tttctcgcaa cctgcaccga
      781 aaagcaccgg acgcgaatac tttaactacg gctggctgga gcgccattta cgccattttc
      841 cgggtgttga tccccgtgat gttcaggcca cactggcaga actcaccgcc gtgaccattt
      901 ctgaacaagt tttgttgagc ggtggctgcg aacgattgat ggtatgtggt ggaggtagtc
      961 gtaatccgct actcatggcg cgtctggcgg cattactgcc aggcacagaa gtcaccacca
     1021 ccgatgccgt tggcattagt ggcgatgaca tggaagcatt ggctttcgcc tggcttgcct
     1081 ggcggacgct ggcgggatta ccaggaaatc tgccttccgt cactggcgca agccaggaga
     1141 cggtactggg ggctattttc cctgctaacc cgtgatatca gagttaactg aatttttctt
     1201 accgcgtctg ccgttacact tggaacgtta gggagggcgt attgccctcc agaccaggaa
     1261 agtcttcggg aaatgtctat gaaaaaactg ttactgatct gtttgccggt gcttctcacc
     1321 ggctgtagcg cgttcaatca actggttgag cacatgcaaa ccgctacgct ggaataccag
     1381 tgtgatgaaa aaccgttgac ggtcaaactg aataatccgc gccaggaagt cagttttgtt
     1441 tacgataatc aactactgca tctcaaacag ggcatttcag cgtctggtgc acgttacact
     1501 gacggaatct atgttttctg gtcgaaaggc gatgaagcga ctgtctataa acgcgaccgc
     1561 atcgtattga ataactgtca gttacaaaat ccacagcgtt gagatttttc caggggcggc
     1621 gcacaatagc gtcacccact gacaatccgt aaagaaaacc atgtctgata acgacgaatt
     1681 gcagcaaatc gcgcatctgc gccgtgaata caccaaaggc gggttacgcc gtcgcgatct
     1741 tcccgccgat ccattaaccc tttttgaacg ttggctctct caggcgtgtg aagccaaact
     1801 ggcagaccct accgcgatgg tggtcgctac cgtggatgaa catgctcagc cttatcaacg
     1861 catcgtttta ctcaaacatt acgacgaaaa aggcatggtg ttttacacca acctcggtag
     1921 ccgtaaagca catcaaattg aaaataatcc gcgcgttagc ctgctgttcc cgtggcatac
     1981 ccttgaacgc caggtgatgg tgatcggtaa agcagaacga ctttcgactc tcgaagtgat
     2041 gaaatatttt catagccgcc cgcgtgatag ccagattggt gcatgggttt cgaagcagtc
     2101 cagtcgcatt tctgcccgcg gtatccttga aagtaaattc ctggagctga agcagaagtt
     2161 tcaacagggc gaagtgccat tgcccagctt ttggggcggt tttcgcgtca gccttgaaca
     2221 gattgagttc tggcagggcg gtgagcatcg cctgcatgac cgctttttgt accagcgaga
     2281 aaatgatgcg tggaagattg atcgtcttgc accctgaaaa gatgcaaaaa tcttgcttta
     2341 atcgctggta ctcctgattc tggcacttta ttctatgtct ctttcgcatc tggcgaaaag
     2401 tcgtgtaccg gcaaaggtgc agtcgttata tacatggaga ttttgatggc aagcagtaac
     2461 ttgattaaac aattgcaaga gcgggggctg gtagcccagg tgacggacga ggaagcgtta
     2521 gcagagcgac tggcgcaagg cccgatcgcg ctctattgcg gcttcgatcc taccgctgac
     2581 agcttgcatt tggggcatct tgttccattg ttatgcctga aacgcttcca gcaggcgggc
     2641 cacaagccgg ttgcgctggt aggcggcgcg acgggtctga ttggcgaccc gagcttcaaa
     2701 gctgccgagc gtaagctgaa caccgaagaa actgttcagg agtgggtgga caaaatccgt
     2761 aagcaggttg ccccgttcct cgatttcgac tgtggagaaa actctgctat cgcggcgaac
     2821 aactatgact ggttcggcaa tatgaatgtg ctgaccttcc tgcgcgatat tggcaaacac
     2881 ttctccgtta accagatgat caacaaagaa gcggttaagc agcgtctcaa ccgtgaagat
     2941 caggggattt cgttcactga gttttcctac aacctgttgc agggttatga cttcgcctgt
     3001 ctgaacaaac agtacggtgt ggtgctgcaa attggtggtt ctgaccagtg gggtaacatc
     3061 acttctggta tcgacctgac ccgtcgtctg catcagaatc aggtgtttgg cctgaccgtt
     3121 ccgctgatca ctaaagcaga tggcaccaaa tttggtaaaa ctgaaggcgg cgcagtctgg
     3181 ttggatccga agaaaaccag cccgtacaaa ttctaccagt tctggatcaa cactgcggat
     3241 gccgacgttt accgcttcct gaagttcttc acctttatga gcattgaaga gatcaacgcc
     3301 ctggaagaag aagataaaaa cagcggtaaa gcaccgcgcg cccagtatgt actggcggag
     3361 caggtgactc gtctggttca cggtgaagaa ggtttacagg cggcaaaacg tattaccgaa
     3421 tgcctgttca gcggttcttt gagtgcgctg agtgaagcgg acttcgaaca gctggcgcag
     3481 gacggcgtac cgatggttga gatggaaaag ggcgcagacc tgatgcaggc gctggtcgat
     3541 tctgaactgc aaccttcccg tggtcaggcg cgtaaaacta tcgcctccaa tgccatcacc
     3601 attaacggtg aaaaacagtc cgatcctgaa tacttcttta aagaagaaga tcgtctgttt
     3661 ggtcgtttta ccttactgcg tcgcggcaaa aagaattact gtctgatttg ctggaaataa
     3721 tgcattaagt ggaaaggggg agtgagaaat cactccccct ggtttttata cagggaacat
     3781 gatgaaaaat attctcgcta tccagtctca cgttgtttat ggtcatgcgg gtaacagtgc
     3841 ggcagagttt cctatgcgcc gcctgggcgc gaacgtctgg ccgctgaaca ccgttcaatt
     3901 ttctaatcac acccaatacg gcaaatggac tggctgcgtg atgccgccca gccatttaac
     3961 cgaaattgtg caaggcattg ccgccattga taaattacac acctgtgatg ccgtgttaag
     4021 tggctatctg ggatcggcgg agcagggtga acatatcctc ggtatcgtcc gtcaggtgaa
     4081 agccgcgaat ccgcaggcga aatatttttg cgatccggta atgggtcatc cggaaaaagg
     4141 ctgtatcgtt gcaccgggtg tcgcagagtt tcatgtgcgg cacggtttgc ctgccagcga
     4201 tatcattgcg ccgaatctgg ttgagctgga aatactctgt gagcatgcgg taaataacgt
     4261 cgaagaagcg gttctggcag cgcgcgaact cattgcgcaa gggccacaaa ttgtgttggt
     4321 taaacacctg gcgcgagctg gctacagccg tgaccgtttt gaaatgctgc tggtcaccgc
     4381 cgatgaagcc tggcatatca gccgtccgct ggtggatttt ggtatgcgcc agccggtagg
     4441 tgttggtgat gtgacgagcg gtttactgct ggtgaaactg cttcaggggg caacgctgca
     4501 ggaggcgctg gaacatgtga ccgctgcagt ctacgaaatc atggtgacca ccaaagcaat
     4561 gcaggaatat gagctgcaag tggtggctgc tcaggatcgt attgccaaac cagaacatta
     4621 cttcagcgca acaaagctct gaaatatctc atttcggccc acaacgacct gtgggccgat
     4681 ggatattact ttaagccttc cgctgacagc gcgtcttgta cttccggacg ttcagccata
     4741 cgttgcataa atgctgcaat gtgctctaac ccttccagat tcagtttcac cgcgtatgcc
     4801 cagcgcagaa ccgtaaacag ataggcatca gcaattgtaa atctttgccc gcagatccag
     4861 tgctcatcct tcagtgcctc gttcacatat tgcagcttct tctccagctg cgcgcgaact
     4921 gtcggtttgt actcttccgg tgtatcaggg cgaaacagag gtgtgaaacc tttatgcagc
     4981 tcggtggcga tgtaattcag ccattcgatg gttttatagc gggaaatact gtttaccggt
     5041 gccagcaact ggcggtcggg gacgctgtcg gcaagatact gcataatcgc tacgccttcc
     5101 gtcagcaaag taccgtcatc cagcagcaat gcaggcacct gccccttagg gttaacggca
     5161 aagtaatcgt caccgttttc gagacgtttt ttcattaaat ccacactgac gagggtaaaa
     5221 tcctttccgc tctcacgcag ggtgatatgg gaagcgagag agcaggcacc cggtttgtag
     5281 aacaatttca tcggtaactc cttttgtctg tggtttaaag tatggtagtg cgttgctgaa
     5341 caaaaaaaaa gccgctaacg aaaagttagc ggcttgtaaa agagtttccc tgaattttac
     5401 gctacagcag ctttcgccgc tttgtctgca gcgtcatcct gcgtcatacg gtgcagttta
     5461 ggcgcggtca gcagcatcag tactgcaata atggcagtag cgacaccaat ctgcaagaat
     5521 acgcgaccat aaacttccag tgacatcagc gggtcggtaa cgttatccgg cacagccatc
     5581 atacccgcaa cataaccacc aatcaggttt gcaccggcag tggtcaggaa ccagctaccc
     5641 ataatgaagc ccatcagacg ctgcggaacg agttgtgcaa ccattgccag acccagacca
     5701 gagatcatca gttccccgat gctctgcagg ccatagcttg cgaccagcca gcttacagac
     5761 acgataccag cgtcagacgc gaatttcgca cccagcggca gaatcaggaa cgcaccagaa
     5821 cacatcacca tgccgattgc aaacttggtt ggcatcggca gggtatcgcc catcttgtta
     5881 tagatagcgg ccagaatcgg actaccgatg atgatccaga acgggttcag tgcctgatac
     5941 tgctcaggtt ctacggccag acccagaatg gagtgctcaa cgttacgaat cgcaaagaag
     6001 ttcagtgatg tcggcatctg gctgtacagc acgaagaaga tgattgcttc gagcatcagg
     6061 atgaaggcaa cgatcatttt acgacgcgca gcacctttca tcgcgaaggc ttctttaccg
     6121 aagataacca cgataccgaa ggcaacaacg cccagcgcca tacgcgcaac ttcctgattg
     6181 tgcagcagcc aggtggcgat agcgatcagt gccacaacac caataatggt tagcagcagg
     6241 ttacggtagt tgataggctc gaagtctggt tttgaaccgt actgtttaac ccagcgttgg
     6301 cagaaggcga agttaacgat agtgatcagc aggcctacaa cgctcaatgc aaacgcaacg
     6361 ctccagccgt atttcgcggc cagccacggc gtagcaatca tagagaagaa agagccgatg
     6421 ttgacggaca tgtagtacat ggtgaatgca ccgtccagac gcgggtcgtt tttctcatag
     6481 catgtagaaa gcagagaaga cgggttagcc ttaaacaggc cgttaccgac cgcaatagcc
     6541 gccataccca tataaacaat accggcgtcg tgaccagacc aggcaaccag agcataacca
     6601 atcgccagca caatagcgcc gagcataatt acgcgtttag tacccagtac cttgtcacct
     6661 aaccagccgc cgatagcgac cagaccataa accagggcac taaaggaaga gaaaagggtg
     6721 attgaatccg cttcagacat acccagttgt ttaaccaggt aaacagccat aattccttgt
     6781 aggccgtaat aaccaaaacg ttcccataac tcaatcgaga agatgagata gaacgccttc
     6841 ggttgtttga aagcgttcaa actgacgctt tcagttggtt tttggtttgc agtggacact
     6901 tttacctctt tttttacatc ccatattaac gggggtgctt tctcgcgtta tgtctaaaaa
     6961 acatacgcgc aattgttata gtgggagggg aaacggcagg taatgttcac tatcttgcac
     7021 tttcagacaa gtcctttgta ataatctgtt acatataact gaggcgggga attcaaccat
     7081 tccgggaaat gttatccagc gttaaatttt tcaaaaaacg taacggcaga ttttttcact
     7141 gcactgagag gggggtgatg gcttaacggc gatatgttct gctatttagc gtttttacta
     7201 gtgagatagt ccagtttctg aaaaatagcc agtgtaatgt tttgtaggtc aatatttgtt
     7261 gcgtgtgtaa atttaaatga ttttaacctg atttttgcaa tgtttttaca ttgccacagc
     7321 atcgtgatct tgatcacgaa tgaatagaaa atttcgctat taaaaaaaca attaacctgc
     7381 atttttggtt atccgcaaac ggattacctg atgataacgc taagcaatgg catcattcac
     7441 gttaaaaaga tgttttaaaa gaggataaag aaaggttatc aatggggtaa tcggtgttac
     7501 cccttttctt cagatgtcaa ctttctcttt gtattcacaa agatcttcaa taatacaaga
     7561 gccacagcgg ggcttgcggg caatgcaggt ataacgcccg tgcaggatca accaatggtg
     7621 gcagtcgact ttaaactctg ccggaaccac ttttagtagc ttttcttcta cctgttcgac
     7681 atttttcccc ggcgcaaatt gagtacgatt acaaacgcgg aaaatgtgcg tgtcgacagc
     7741 aatagtcggc cagccgaatg cagtgtttaa tacgacgttg gctgttttac gacctacgcc
     7801 gggcagggct tcaagcgcag cacgatcttc cggaaccaag ccattatgct gctccagtaa
     7861 gatacggcag gttttgatga tattttctgc tttgctgtta taaagcccaa tcgttttgat
     7921 ataggtttta accccttcaa cgcccagttc aagcatcgct gcaggcgtat tcgccaccgg
     7981 gtagagtttc gccgtcgcct tattaacact gacatcggtc gcctgagcgg aaagcagtac
     8041 ggcaatcagc aattcaaaag gcgaactgaa attaagctcg gtggtgggat gaggattgtt
     8101 ctcacgcagg cgagtgagga tctccaggcg ttttgctttg ttcatcagac attccctgtt
     8161 tcaccgtttg gcaatgcacg ttcggcagct gcttcagtac ggcgcttttt cattctttca
     8221 tcaatcaggt attttcctgc cagcatcagt cccaggccaa taaatgcacc tggaggcagc
     8281 atcgccagca ggaaagggga gtcggtgtgg aaaatttcca cgcgtaatac ttttgcccag
     8341 ctacctaaca gcgcatctgc accgtcaaac aatgtgccat tgccgataat ttcgcgtagt
     8401 gaacccagca cgaacatggc gcaggttgcg cccataccaa ttgaaaagcc gtccagtgcc
     8461 gaaagcgccg gacctttttt ggcggcgaag gcttcagcgc ggcccacaac gatacagtta
     8521 gtgacaatca gcggaataaa aatccctaat gattgataca ggccaaaggc gtaggcgttg
     8581 atcagcatct gtacagcgct gaccaccgag gcgatgatca tcacgtaaat gggaatgcgg
     8641 atctcggctg gcgtccagtg acgcagcgtc gaaatggtca ggttggtcag cgtcagtacc
     8701 agcgtagtcg caagtcctaa acccagagcg ttagtggcag tggacgtgac cgccaacaga
     8761 ggacaaaggc cgagcaactg gaccagcgca gagttgtttt tccacaaccc ctgaacaata
     8821 acgtctttaa tttcgctcac gctttattct ccacaggcag gaagttgaga aagttgtgcc
     8881 ggtaacgtct gagcgtacaa tccggcgcgt tttaccgcat taaccaccgc gcggggagta
     8941 atcgtcgcgc cggtgaactg gtcgaaatca ccaccatctt tcttcaccgc ccagtgcgca
     9001 tcatctgcac cactgatttt tttacccgca aaatgggtga tccagtcaga aaggcgcagt
     9061 tcgattttat cgccaagccc tggcgtttcg tggtgctctg tcacgcgcgt gccaagtacc
     9121 gtgccgttaa aatcggctcc caccagcagc tgaatcgcac cggaatagcc atctggcgcg
     9181 gttgcttcca gaacggcggc taccggtttg tcatcctgtt tggcgatgta aacccgatgc
     9241 tcacctttac ctaactctgg cgcagttacc agatagcaac tctgtgccag cgcattgtta
     9301 tagcgttcgg ctggcagcac ctgatcaaat aatgcctttt gttgcagact ggcctgttca
     9361 gcaatcgtcg ttttggtcat ctggttgatg gccgcagtta accctgttga acccgctgca
     9421 aatagcgcta acgtaatgcc gtgttttcgg atagttttca gcatggttta ccctttgcga
     9481 tggccgtaga cgcgcggacg cgtgtagtaa tcgatcagag gaaccgtgat gttcgccagc
     9541 aggacggcaa aagccacgcc gtcaggatag ccgccgaaac tgcggatcaa ccagactaat
     9601 aagcccgcca gcgcgccgaa aataagacga ccacgattgg tcgtagaagc ggtaaccggg
     9661 tcagtcaaaa taaagaatgc gccgagcatg gtcgcaccag acagcagatg aatttgcggt
     9721 gctgccagtg tttctggtga gaacaaccag cccaacgttg cgcataacgc cagcgttact
     9781 aagaagctga ggggaatatg ccagcgaatc gctttctgcc atagcaacca caggccgcca
     9841 gccagccagg cgagatttac ccattgccaa ccagcgcccg ccagaatacc gctgtagatc
     9901 ggatattgca taatctgttc aaccgaatga ccggcacgga cagaggtttt aaatgtatcc
     9961 agcggtgtcg cctgactaat gccatcaata cctaagcgta gtgtgttcat atcaccacca
    10021 ctggcggtat gtccgctgaa aataacctgt atggcgtcga taaaaccagg gatgttgacc
    10081 gcaatttcat gtggcggtaa ccagctggtc atctgcaccg ggaaggagat cagtaagacc
    10141 acataaccaa tcattgccgg attaaacggg ttttgcccca gaccgccata caactgttta
    10201 gcgatgatca ccgcaaacac cgtacccagc acgaccatcc accatggcgc gaggggggga
    10261 atacttaccg ccagcaataa gcctgtcagc aatgctgagt tatctttcaa cgttgcggct
    10321 accgactgct tgcgtagttt gagtacgaga gcttcggcta acagagcact aaccgatgcc
    10381 aacaggatct gaacgagagt accccaacca aaaaaccaca gttgcgctgc gattcctggc
    10441 acggctgcga gcaacaccaa cagcataatg cgcgatgtct ggcgctggtt atgggtataa
    10501 ggggagctag ctattctgaa taccatttag tcctcgttta caaccttctg ctgggcggct
    10561 tttttggcct gagcgcgggc aatagccgcg gcaactgccg ctttgcgcgg atcaacctgt
    10621 tcttctggtt ctgcgttagt ctgttgctgt tccagcttgc gcgctttggc acgggcaata
    10681 gcggcttcga cggcggcttt gcgcggatca acctgttctt ctggttccgc attagcctgt
    10741 tgctgttcca gtttgcgcgc tttggctcgg gcaatagcgg cttcgacggc ggctttgcgc
    10801 ggatcaacct gttcttctgg ttccg
//
LOCUS       DAZFWL020000147        10823 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFWL020000147 DAZFWL020000000
VERSION     DAZFWL020000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.147"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1019..3208
                     /gene="zorA1"
                     /locus_tag="ACHKNP_RS20650"
                     /old_locus_tag="pgaptmp_004130"
     CDS             1019..3208
                     /gene="zorA1"
                     /locus_tag="ACHKNP_RS20650"
                     /old_locus_tag="pgaptmp_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorA1"
                     /protein_id="HGU4706196.1"
                     /translation="MSWLNSILVTLTSVEPYKVPVTVIVTVTFAFVCFIFFYLLRSIR
                     IIYGLKKYTRSINSIEKSAPEVQLEHLKSLFQRSELKHAWNEFEESLHSQYELENGEE
                     KIVRIRATAPSASFFSEQQLVDIPLNTEFFKHLPGILTGMGIIGTFYGLMIGLNHFDP
                     STPEQVSSSVNNLLRDVLYAFLGSAFAIFASILVTWLEKLSIAKSYKYLEKFTAALDS
                     LYDSGVGEEYLASLVKSSNESATQARHLKESLVTDLRDMLLHLAESQKIENERLANTL
                     SATYRESGSQFADQVSGAIENSLKSPLDKIAGAVQTASGDQSGMVQNMLQNVLTAFMA
                     KLDTTFGQQFTNLNEMMGQTVGAIQTMQTGFSALLQDMRQVSDDSRQGSAQLIEQLLS
                     EMKSGQQALQAGMNDMLTSLQVSVAKIGAEGEGAGERIARQLEKMFADSEAREKAQAE
                     HMAAFVEAIQNSVQQGQSATMEKMAASVGALGEQLGSLFGQIDKGQQQISATQQANQQ
                     SLHEQTQRVMSEVDDQIKQLVETVASQHQGTTETLRLLAEQTNRQIQDMQAGADKMRL
                     AAERFEHAGERVSEANHLTADVLNKAQSAGSSLSLATSELTSVVADYRNNREAVSKSI
                     AMLELLAANTQSEQTTRNQFIADLKQHGERLQSYNREAQVFMENVSDVLGKGFEDFSE
                     GVSRSLDKTLGKLDVEMAKASNLLAGSVEQLGESVSELDDVLSRVRT"
     gene            3222..3962
                     /gene="zorB1"
                     /locus_tag="ACHKNP_RS20655"
                     /old_locus_tag="pgaptmp_004131"
     CDS             3222..3962
                     /gene="zorB1"
                     /locus_tag="ACHKNP_RS20655"
                     /old_locus_tag="pgaptmp_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorB1"
                     /protein_id="HGU4706197.1"
                     /translation="MFGNAFGVKKRRSDEAEKPFWISYADLMTAMMVLFLVVMVASLS
                     SVTQRIQRAEQGEKARGQDISRLCERLELHARNVNKNIVVDCHDNRISFGEAGRFAHN
                     QFFLNAEGQKALQDVVPLVLEASNSEEGKKWFKQIVIEGFTDTDGSYLYNLHLSLQRS
                     EWVMCSLLDSRSPLQKNISAEQQLQIRKLFLAGGVSFNNAKESKEASRRVELRMQFFG
                     LKDKRDKADEVDFPPVVNKEVCQLVMPL"
     gene            3959..5641
                     /gene="zorC"
                     /locus_tag="ACHKNP_RS20660"
                     /old_locus_tag="pgaptmp_004132"
     CDS             3959..5641
                     /gene="zorC"
                     /locus_tag="ACHKNP_RS20660"
                     /old_locus_tag="pgaptmp_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorC"
                     /protein_id="HGU4706198.1"
                     /translation="MIPALNSLSQRIAARLSSSQRDDHYLHNDFHALTAATLDMEKRF
                     DRAERIPSPPQEMRLEALRRFRLAQELTEREWRMVFYGLADNDPLYPDQPILLEDDTF
                     FPRVNSAIKKRIETKTLKRRDWAALCSSYFAYQHQSPETNPHWCVLRGHIAQGYLVVK
                     AAIRREKSWMKTIEFYHDIFTPQAGGVISRQLLAGENNSLSSLEKIAQIPDSSWLWKR
                     IFTVLLAQLDTLDDPQFLSKISWLLGLAAQWVRFRDDIMTATLTRYYHSGYRDQSHPA
                     LKQAALEYWDNPQLKSQQNKWHQYVSESVAAMVRGWLAKQDLTHFFELLRGNGDVDQA
                     RLHYWLRFANQMGFTRIIMGSDAWQDRGSDFVKFREENKGRLSYLRGGRNFDNAMVMQ
                     INDYLFVEFSGTGNAMYAYQIGHAPFNPESRTLDINIHLKDQGRCALRLPHAPRAEGY
                     NKVRITGWMLKYDDELRKLGIRWMAEEPVRFVDKKVPPPVAMSDIKIINPLRDTAIQR
                     LVKCSSCIVSDNRHKGGILSVQLITPDDTVERELLRLGFAPVAKEPHRYWIK"
     gene            5641..8883
                     /gene="zorD"
                     /locus_tag="ACHKNP_RS20665"
                     /old_locus_tag="pgaptmp_004133"
     CDS             5641..8883
                     /gene="zorD"
                     /locus_tag="ACHKNP_RS20665"
                     /old_locus_tag="pgaptmp_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorD"
                     /protein_id="HGU4706199.1"
                     /translation="MLKRLLSKLTGNRQQIEHHLKNQYQVEENGLSFPLSLVDDSQLW
                     ALASWLEQLAEEDYLISLTDRWLLSWEALYRLLEDEEHASSLPLIGVPDILPLRASLS
                     SRGALSDSDFRVWIAEWATFPARKPIRFSRTGAILTHDNQQYLLSRENWALLQATEQL
                     SAQQIQTPGETTNQLGWAAIRKCAKLAAAKFDDYLEKTHVIKPTSLSLRLRKATVADT
                     AVIEIEPHFEDQPANWLGSFDKNLQVHDSYRIPGENGELSHVIIPPEVKEVLNSIHSI
                     PGRRVAGSEALSFVRNPYTFLGEDAASVIAPEEHEQALFDARIFFHHFRLIPQLNAEN
                     KIAEVTLILEPVSPVPQPEITFVFSAPWELDKFIQQLGISVAAQMPAGSWQGYELELS
                     QFTEQQWHDCQALLTRWQQEIEGKEFSDVLDIAKYGDRVIGIGEFEKISSPWLTKAQS
                     ENWLPDDIDFSAFSIETLSGWQPENPHHFDELQEKITQAEAAGETYITTPWNDSQLPL
                     DAAKTFSKNWEKQQITANEYQGNVADKTARAVLKIEQNIEETAYIKQRRNSLLNARHA
                     EPEIPLSLKEHIRLKDHQREGVAWLQQLFLRSPEETAGCLLADDMGLGKTLQILSFLV
                     WFIEKFPQEPPNLIVAPVSLLDNWERELNNFFYTAGIPVLKLYGETIKAVKYPKQAIP
                     AHLQSKGIKNLLKPGWQGEAKIILTTYETLRDQEFSLARQPWSIMVCDEAQKIKNPAA
                     LITHAANAVQARFKVACTGTPVENTLVDLWSLFDFAQPGLLGALNEFGKQYVRPIENE
                     DGRDTERLESLRALIEPQTLRRTKEEVARDLPQKIEVESCKQLTLSGVQKQLYLSSVA
                     NWQQQQALREGMQQAGTGMLGLLHRLKLICAHPAIVNPEPRFRDNSPKLNWLLKILTE
                     IKHTSKDKVIIFTELRDLQRELQHAIHQNFGFRPVIINGDSSTKSQSQNSRQRLIDDF
                     QAQPGFGVIILSTVAVGFGVNVQKANHVIHFTRCWNPAKEDQATDRAYRIGQTKNVYV
                     YYPTVRDTEITTFEETLDDLLQRRRALARDMLCATPDLNCADFETILKGA"
     gene            complement(9043..9209)
                     /locus_tag="ACHKNP_RS20670"
                     /old_locus_tag="pgaptmp_004134"
                     /pseudo
     CDS             complement(9043..9209)
                     /locus_tag="ACHKNP_RS20670"
                     /old_locus_tag="pgaptmp_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(9460..10023)
                     /locus_tag="ACHKNP_RS20675"
                     /old_locus_tag="pgaptmp_004135"
     CDS             complement(9460..10023)
                     /locus_tag="ACHKNP_RS20675"
                     /old_locus_tag="pgaptmp_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4706200.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHGCKHSMCMEIYRLLSETQSMLAGYYWVMEYTQNKGLHIHFVGYLDGQRHKNS
                     YQISRQLGDIWRRITEGDGYFHLCRAKDKYPVRIDHVMHYSDKSAVDNLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
BASE COUNT         3151 a         2548 c         2450 g         2674 t
ORIGIN      
        1 ataaccataa ttaatggaaa agtgcagaaa attattccac tgaatgcttt taatacagtt
       61 ggttagctaa aaaaatatca ttttttgatt tcagtcattg aaaattgcaa tgcgtgacga
      121 ttcaacagca tttaagtgag tttcaggata tgggaaggac atgaaaggag gagcgcaata
      181 aaacacacca gaagaaggag gagaaaatgc ttccctgtat atttattttg tcaggccatg
      241 gcctttctcc agcaaccgat caacgccaca tacctgcagc gacactcaac agaagtgaga
      301 tcccatgcaa tatcatcatc acagggccag cggaaggtcc cttgtacggg cggtaggtac
      361 acagccatat gccatgttta tcctggcaca ggacttttat gcaagtatag gctttgttga
      421 tgcagaacca tcataccatg caaagctaag ttcgttctgg acatactgta tcaggatgcc
      481 aatccccgac ttccatcgac aggtttacga tcaggaatac attatcaacc gtcagtattg
      541 cgaaactctc agaagacatc tgaaaacata tttcagaaga gaatccctgt tctacatact
      601 tttagtcggt agcctcatgc aggctcgcac tttaccggca gcattccata atttgtgatc
      661 ctactcatta agaaatcgtt atcaggtatt gttcatgaaa atcctcagga tggatcacaa
      721 cggaagagta atcatatctt atcgcagatt cagatttaga atgcggcacg taaaatattg
      781 tccaggggac gctgaccacg acacaacatc aagaaatttg cttttgcatg gtaaaaaaga
      841 ggaaactaag aaaatgtctc acctgattat tctgtttcta tcgttcattt attgaacagc
      901 aagagtattc aggtgtaacc gcacgacata gtgacaacca atgcaacata atcaattatg
      961 ttgactatgt tgctgatgac taattttagt atggataata tattaaacga gtaaaacaat
     1021 gtcctggctt aattccattt tagtaacact tacatctgta gaaccctata aggtacctgt
     1081 cactgttatc gtcactgtta cctttgcctt tgtctgcttt attttctttt atttactgag
     1141 atccatcaga attatttatg gattaaaaaa atacacgcgt tcaattaaca gcattgaaaa
     1201 aagtgcgcca gaggttcagc ttgaacacct taagagcctg tttcagcggt ctgaactcaa
     1261 gcatgcgtgg aatgagttcg aagaatcgtt gcactcacaa tatgaactgg agaacggcga
     1321 agagaaaatc gtccgtattc gggctactgc gccgagcgcc agcttcttct cggagcaaca
     1381 actggtcgat attccgctga acactgaatt tttcaaacac ctgcccggta ttctgaccgg
     1441 tatgggaatt atcggtacct tctacggtct gatgattggt ctcaaccact tcgaccccag
     1501 cacaccagag caggtctcca gtagcgtcaa taatctacta cgcgatgtac tgtacgcctt
     1561 cctgggttct gcatttgcta tttttgcttc cattctggtc acctggcttg aaaaattatc
     1621 catcgctaaa agctacaaat atctggaaaa attcaccgca gccctcgact ccctgtatga
     1681 cagcggcgtt ggcgaggagt accttgcctc actggtgaag tccagtaatg aaagcgcgac
     1741 ccaggcacgc catctgaaag agagcctggt tacagattta cgcgatatgt tactacatct
     1801 ggccgaaagt cagaaaatcg aaaatgaacg cctcgcaaat acgttaagcg caacttaccg
     1861 cgaatcaggt tcacaatttg ccgaccaggt cagcggtgcg attgaaaaca gcttgaagtc
     1921 accattggat aaaattgccg gtgcggttca gaccgccagc ggcgatcaaa gcggcatggt
     1981 acaaaacatg ctgcagaatg tactgaccgc gtttatggcg aaactcgaca ccacctttgg
     2041 tcagcagttt acaaacctca acgaaatgat ggggcaaacc gttggtgcaa ttcagaccat
     2101 gcaaaccggt ttctccgcgt tattacagga catgcgtcag gtgagcgacg attcccgcca
     2161 gggcagcgcg cagcttatcg agcaactgtt atcagagatg aaatctggcc agcaagctct
     2221 acaggctggc atgaatgata tgctcaccag ccttcaagtc tcagtggcga aaattggcgc
     2281 agaaggtgaa ggggccggtg aacgtatagc tcgccaactg gaaaaaatgt tcgctgatag
     2341 cgaggcgcga gaaaaagccc aggcagaaca tatggctgct tttgttgaag ccattcagaa
     2401 ttcagtacaa cagggacaaa gcgccacaat ggaaaaaatg gcagcctctg tcggggcgct
     2461 tggtgaacaa ctgggtagcc tgtttggaca gatcgataag ggtcagcaac aaatttcagc
     2521 gactcagcaa gcgaatcagc agtccctgca cgaacagact caacgggtaa tgagtgaggt
     2581 agacgatcag attaaacaat tggtcgaaac tgttgccagc cagcatcagg gaacaacaga
     2641 aactcttcgt ttgctcgcag aacaaactaa ccggcagatt caggatatgc aagctggcgc
     2701 ggacaagatg cgtctggctg ctgaacgctt tgaacatgcc ggtgagcggg tgtcggaagc
     2761 aaaccacctt accgctgatg tgctgaataa agcgcaatcg gcaggttcat cactttccct
     2821 tgccaccagc gagctcacct ccgttgtggc tgattatcgt aacaatcggg aagcggttag
     2881 caaatccatt gccatgctgg agttgctcgc cgcaaatacg cagtcagaac aaaccacgcg
     2941 caaccaattc atcgcagatc ttaagcaaca cggtgagcgt ctacagagct ataaccggga
     3001 agcacaggtc tttatggaaa atgtaagtga cgtgctcggg aaaggatttg aagacttctc
     3061 tgaaggtgtt tcacgcagcc tggataaaac gctgggtaaa ctggacgtcg aaatggcgaa
     3121 agcctctaat ctgctcgcgg gatctgtcga acagctcgga gaaagcgtca gcgaacttga
     3181 tgatgttctg tcacgcgtac gaacctaagg ggtgatggct gatgtttgga aacgcatttg
     3241 gcgttaaaaa acgccgcagc gatgaagcag aaaaaccatt ctggatctcc tatgccgatc
     3301 tgatgactgc aatgatggtg ttatttctgg tcgtcatggt tgcgtcactg agttccgtta
     3361 cacagcgtat tcaacgtgcg gagcaaggcg aaaaagcaag ggggcaggat atttccagat
     3421 tgtgtgaacg cctggaactg catgcccgca atgtgaacaa aaacatcgta gtggattgcc
     3481 acgataaccg tatcagcttt ggcgaagccg ggcgtttcgc tcataaccaa tttttcctga
     3541 acgccgaggg gcaaaaagcg ttgcaggatg tagttcctct tgtactggaa gcgtccaaca
     3601 gtgaagaggg taagaagtgg tttaagcaga tcgttattga agggtttacc gacaccgacg
     3661 gttcgtactt atacaaccta catctctccc tgcaacgctc tgaatgggta atgtgtagtc
     3721 tgctcgatag ccgcagccct ctgcaaaaaa atatctccgc agaacagcag ctccaaatcc
     3781 gtaagctttt cctcgcgggt ggcgtctcct tcaataatgc taaggaaagc aaggaagcca
     3841 gccgccgggt cgagttgcgc atgcagttct tcgggcttaa agataaacgt gataaggccg
     3901 atgaggtgga ttttcccccc gtcgttaata aagaagtttg ccagttggtt atgccactat
     3961 gatacccgcc ctgaattctc tttctcaacg cattgcagcg aggctctctt ccagccaaag
     4021 ggatgaccat tatctgcata atgatttcca cgcgttgacc gcggctactc ttgacatgga
     4081 aaagcggttt gatagggcag aaagaatccc ctcaccgcca caagaaatgc ggctggaggc
     4141 tctacgtcgc tttcgcctgg cacaagaact cacggaacga gagtggcgga tggtgtttta
     4201 tggcctcgcc gataatgatc cattatatcc tgaccagccg atcctgctgg aggatgatac
     4261 atttttcccc agagtaaaca gtgcaatcaa aaaacgtatt gaaaccaaaa cgttgaaacg
     4321 ccgggattgg gcagcacttt gttcaagcta ttttgcctac caacaccaat cacccgagac
     4381 aaaccctcac tggtgcgtac tgcgggggca tatcgctcag ggttacctgg tagtgaaagc
     4441 cgctatacga cgagaaaaat cgtggatgaa aaccattgag ttttatcacg acatttttac
     4501 cccacaagcg ggaggagtga tctccaggca gttacttgca ggtgagaaca attcactctc
     4561 atcattagag aaaatcgcgc aaatccctga tagcagttgg ttatggaagc gcattttcac
     4621 tgtgctttta gctcaactgg acacgctcga cgatccccag tttctgagca aaattagctg
     4681 gttgttaggc ttagctgcgc agtgggtgcg tttccgtgat gacatcatga ctgcaaccct
     4741 gactcgctat taccattctg gctatcgcga tcagtcacat ccagcgttga agcaggccgc
     4801 gctggaatac tgggataacc cccagttgaa aagccagcaa aataaatggc atcagtatgt
     4861 ctccgagtcg gttgccgcaa tggtccgggg ctggttggca aaacaggacc tgacacattt
     4921 ctttgaactg ctgcgcggta atggcgatgt cgatcaggct cgactgcact actggctgcg
     4981 tttcgctaat cagatgggat ttactcgtat catcatgggg tccgatgcct ggcaagatcg
     5041 tggtagcgat tttgtgaaat tccgcgagga gaataaggga cgattgagtt acttacgagg
     5101 tgggcgtaac tttgataacg ccatggttat gcaaatcaac gattatctgt ttgtcgagtt
     5161 ttccgggaca ggaaatgcga tgtatgccta tcaaataggt cacgcgccat ttaaccctga
     5221 atcacgcacg cttgatatca acattcatct aaaagatcag gggcgatgcg cactcagact
     5281 cccccatgca cctcgtgctg aggggtataa caaagtccgg ataaccggct ggatgttgaa
     5341 atacgacgat gagctacgta agttgggcat tcggtggatg gctgaagaac ccgtcaggtt
     5401 tgtcgataag aaagtgcccc ctcccgtcgc tatgtctgac atcaaaatca tcaacccgtt
     5461 gcgtgatacg gctatacaac gcctggttaa atgtagttca tgtatcgtca gcgataatcg
     5521 acataaaggt ggcatactgt ccgtacaact cattacacct gacgacaccg tagaaagaga
     5581 gttgttacga ctaggctttg cacctgtagc taaagaaccg catcgttact ggatcaaata
     5641 atgctgaaac gtctgttaag taaactgacg ggaaaccgtc agcagataga acaccaccta
     5701 aagaatcagt atcaggttga ggaaaacggt ctcagctttc cgctatcgct ggttgatgat
     5761 tcacagcttt gggcactggc atcctggctt gaacaacttg ctgaagaaga ctatctgata
     5821 tcacttacag atcggtggtt attgagttgg gaggcactat atcgcctgct ggaggatgag
     5881 gaacatgcca gcagcctgcc tcttatcggt gtacctgaca tattgccctt acgcgcaagc
     5941 ctgagttcac gaggagcatt aagcgatagc gattttcgcg tctggattgc tgaatgggct
     6001 acatttccag cccgcaagcc gattcgtttc agtcgtaccg gtgccatttt aacgcatgat
     6061 aatcagcaat acctgttatc gcgggaaaac tgggcgctct tacaggcaac agaacaactc
     6121 agtgcacagc aaattcagac tcccggcgaa accactaacc agctgggatg ggccgctatc
     6181 cgcaaatgtg cgaaactcgc cgccgccaaa tttgatgatt atttagaaaa aacgcatgtc
     6241 atcaaaccaa cttcattgtc attacgtctg cgtaaagcaa cggttgccga tactgctgtt
     6301 atcgaaattg agccccattt cgaggaccag cctgctaact ggctgggcag ttttgacaag
     6361 aacttacagg ttcacgatag ctaccgcatc ccaggagaga atggcgaact cagtcacgtc
     6421 atcatccctc ccgaagtgaa agaagtcctg aattcaatac actcaatccc agggcgtcga
     6481 gtggccggaa gtgaagcact ttcctttgtt cgcaatcctt ataccttcct gggtgaggat
     6541 gccgccagtg ttattgcccc tgaagaacat gaacaggcac tgtttgatgc caggattttc
     6601 tttcatcatt tcaggttgat accgcaactg aatgcagaaa ataagatcgc agaagttacg
     6661 ctgatacttg agcctgtttc acctgttccc cagccagaaa tcacttttgt gttctcagcg
     6721 ccctgggagc tggataaatt catacagcaa ctcggtatta gcgtcgctgc acagatgcct
     6781 gccggttcat ggcaaggtta cgagctggaa ttaagtcagt tcaccgaaca gcagtggcac
     6841 gattgccagg cactgctaac tcgctggcaa caagaaattg aaggaaaaga atttagcgat
     6901 gtgctggata ttgcgaagta tggcgataga gtgattggca ttggtgaatt tgaaaaaata
     6961 tcctctccct ggctgaccaa agcgcagagt gaaaactggc ttcctgatga catcgatttc
     7021 tccgcgtttt caatcgaaac actatctggc tggcaacctg aaaatcctca ccattttgat
     7081 gaactacagg agaaaattac tcaggcagaa gccgctggtg aaacgtatat caccacacca
     7141 tggaatgaca gccaattacc gttggatgcc gctaaaacct tcagtaagaa ctgggaaaaa
     7201 cagcaaatca ctgctaatga atatcaaggc aacgttgcag ataaaacggc ccgagccgta
     7261 ctcaaaattg agcagaatat tgaagaaacg gcctacatca aacagcgccg caattcactc
     7321 ctcaatgcac gtcatgccga accagaaatt cctctgagcc ttaaagagca tatccgactt
     7381 aaagaccatc aacgtgaagg cgtagcctgg ctccagcaac tcttccttcg ttcacctgag
     7441 gaaacagcag gctgcctgct ggcggatgat atggggcttg gaaaaacgct gcaaattctg
     7501 agcttcctgg tatggttcat tgaaaaattc ccgcaagaac cgccaaatct cattgttgct
     7561 cctgtctctc ttttagataa ctgggaacgt gagctgaaca atttcttcta taccgcagga
     7621 ataccagtat tgaaattgta tggcgagacc attaaagcgg tgaaatatcc caaacaggcc
     7681 atccctgctc atctgcagtc taaggggatc aaaaacctgc ttaaacctgg ctggcaaggt
     7741 gaggcaaaaa tcatcctgac gacctacgaa acgcttcgcg accaagagtt ttctctggca
     7801 cgccagccat ggtccattat ggtgtgcgac gaggcacaaa aaataaaaaa cccagctgcg
     7861 ttaatcacac atgcggccaa cgcggtacaa gccaggttta aagtggcatg cacaggaacg
     7921 ccagtcgaaa acacacttgt cgatctgtgg agcttatttg attttgccca gccaggattg
     7981 ttaggggcac taaatgaatt tggtaaacag tatgttcgcc ccatcgagaa cgaagatggc
     8041 cgcgatacag aacggctgga aagcctgcgg gcgctgatag aacctcagac tttacgccga
     8101 accaaagaag aggttgcacg tgatttacct cagaaaattg aagtagaaag ctgcaagcaa
     8161 ctgacgttgt ctggcgtgca aaaacagctc tacctttcgt ctgttgcaaa ttggcaacaa
     8221 cagcaagcac tgcgcgaagg gatgcagcag gccggaaccg gtatgttagg tttgttacac
     8281 cgactgaaac tcatctgtgc acaccctgca atagttaatc cagagccacg ttttcgtgat
     8341 aactcaccca agcttaactg gctgctgaaa atactcacag aaataaaaca tacctctaaa
     8401 gataaggtca ttatttttac ggagcttcga gatttacagc gcgagcttca acatgcaatc
     8461 caccaaaatt ttggtttccg ccctgtcatt atcaatggtg atagcagcac taaaagtcaa
     8521 agccaaaaca gccgccaacg cctgattgat gattttcagg ctcagcctgg gtttggggtg
     8581 attattctct ctacggttgc tgtcgggttt ggcgttaacg tccagaaagc caatcatgtt
     8641 attcatttta ctcgctgctg gaatccagcc aaagaagatc aggctacgga tcgggcgtat
     8701 cgcattgggc aaactaaaaa tgtgtatgtc tattacccaa ctgtgagaga tactgaaatc
     8761 acaacatttg aagaaacact ggatgatctc ttacaacgcc gacgagcatt ggccagagat
     8821 atgctctgcg ctactcccga tttgaactgt gcggattttg agaccatttt gaagggagcc
     8881 taaccgatat gagcggcaat actatcagtt gccaaacctg aaagaacact tagctgtgat
     8941 gattcgctat tacctaccga tagcttcgga aaaacataaa ttaaaaatgc ccagaggagt
     9001 actattatcg ttagtattat tatccgaacg ctgtattaac ggttatttat tggtgagtac
     9061 tacgttccat ggcaagagtt catcaacagg ttggaagacc attccggcaa aactctcagt
     9121 atatggcgca gataccattc agacagcagg tcccgctcag cccgtacagt agagcgccgt
     9181 tcactaccgt aatcgctacc gaagaacaca ttgattgccc ctcaataatc ctgctgttaa
     9241 catcaaaatt gttggtcggg ttcgatttat ttaattgtgt aaacagggtc tctcagagaa
     9301 aggcccttat gcatcacctg acgttaaaag tcactacacc caagatgacg ttcaaagtgt
     9361 catagtcccc tacactgatg acaaagccgc cctctgctgc ctattaactc aagcacctcc
     9421 caacaactta cagaaactta attctaatac agctgtaatt cagttgtgtc gtggtcggcc
     9481 gcggttactt ttttcaggaa gcctgctacg gcccagaata ataccgtgtt ctttctgatc
     9541 ctgtttagcc agatagctaa gggcgtaacg taagttgtct accgcggact tatcactata
     9601 gtgcatcacg tgatcaatac gcaccgggta tttatcttta gcccggcaca ggtgaaaata
     9661 accatcccct tccgtgatcc gcctccagat atctcccagt tgcctggata tctgataaga
     9721 gttcttgtga cgttgcccat ccaggtagcc gacaaaatga atatgaagcc ctttgttttg
     9781 tgtatattcc attacccagt aatatcccgc caacatcgat tgggtttcgc taagtaagcg
     9841 gtatatttcc atacacatac tgtgcttaca gccatgacca aaactgggcg tgtctttcct
     9901 gtaggcaaaa tcaattctga aaggtaacag tttagaaaaa cgttgaaaca gaccttccat
     9961 gtgttcattc acgtctttca gaatcatgaa atccatttcg taattgggat tagcattata
    10021 cattttatag tccttacttt ataaaactga caggaaagtg gaatcacata aatactgaga
    10081 gtacagaaac gccgtaccgc ggcattaata aacgaggtaa ccagcagcac agacaacctg
    10141 ataaaactgt ttcgtattac ctcattactc caaagaggta atatttcaga gtacagtaag
    10201 actcacttaa actgaggacc agtacgaatt acaatggtaa aaacacacct catagaattg
    10261 agtttaagga agtaccattc aatcacttac aggaagtaat ctgacattca caaaagccag
    10321 tatcgggtag ccacaggcaa caccgctatt aatacttctg taccattaag taaaaacagc
    10381 gacccagctt gaggcctcta cagatattta acttcaccat aaaaatgatg gtatacagac
    10441 cataccccac acaccataag aatattattt catacaggaa agattatatt ttttcatacc
    10501 tataaactgt aacagagcca taacatactc taatagtgtt ccggtacgta accagtgtta
    10561 agtgcgtaca agacaacata acagatcaca taacttccac taataatact aattaaccta
    10621 aattatttat tgaacacgaa aacaatgaag gccccacctc acctcagact aaagccgaca
    10681 atatcctgag taacactcta tccaatagta aatagtggtt acaccagtta tatttactga
    10741 ctgacctgta ccagagtttt ctaatacaac gctgacatgg taaagacatt agtctcctcc
    10801 gcaagtagca gaggagacta tat
//
LOCUS       DAZFWL020000148        10778 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFWL020000148 DAZFWL020000000
VERSION     DAZFWL020000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.148"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            125..838
                     /gene="yghS"
                     /locus_tag="ACHKNP_RS20680"
                     /old_locus_tag="pgaptmp_004136"
     CDS             125..838
                     /gene="yghS"
                     /locus_tag="ACHKNP_RS20680"
                     /old_locus_tag="pgaptmp_004136"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4706201.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            870..1628
                     /locus_tag="ACHKNP_RS20685"
                     /old_locus_tag="pgaptmp_004137"
     CDS             870..1628
                     /locus_tag="ACHKNP_RS20685"
                     /old_locus_tag="pgaptmp_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706202.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1674..3023
                     /locus_tag="ACHKNP_RS20690"
                     /old_locus_tag="pgaptmp_004138"
     CDS             1674..3023
                     /locus_tag="ACHKNP_RS20690"
                     /old_locus_tag="pgaptmp_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HGU4706203.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            3023..3847
                     /locus_tag="ACHKNP_RS20695"
                     /old_locus_tag="pgaptmp_004139"
     CDS             3023..3847
                     /locus_tag="ACHKNP_RS20695"
                     /old_locus_tag="pgaptmp_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706204.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            3859..4419
                     /locus_tag="ACHKNP_RS20700"
                     /old_locus_tag="pgaptmp_004140"
     CDS             3859..4419
                     /locus_tag="ACHKNP_RS20700"
                     /old_locus_tag="pgaptmp_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU4706205.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4450..5520
                     /gene="lptF"
                     /locus_tag="ACHKNP_RS20705"
                     /old_locus_tag="pgaptmp_004141"
     CDS             4450..5520
                     /gene="lptF"
                     /locus_tag="ACHKNP_RS20705"
                     /old_locus_tag="pgaptmp_004141"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU4706206.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5517..6596
                     /gene="lptG"
                     /locus_tag="ACHKNP_RS20710"
                     /old_locus_tag="pgaptmp_004142"
     CDS             5517..6596
                     /gene="lptG"
                     /locus_tag="ACHKNP_RS20710"
                     /old_locus_tag="pgaptmp_004142"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU4706207.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     VLVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6632..7804)
                     /locus_tag="ACHKNP_RS20715"
                     /old_locus_tag="pgaptmp_004143"
     CDS             complement(6632..7804)
                     /locus_tag="ACHKNP_RS20715"
                     /old_locus_tag="pgaptmp_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGU4706208.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYSSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQINAIIQAFATLKQ"
     gene            complement(7804..8052)
                     /locus_tag="ACHKNP_RS20720"
                     /old_locus_tag="pgaptmp_004144"
     CDS             complement(7804..8052)
                     /locus_tag="ACHKNP_RS20720"
                     /old_locus_tag="pgaptmp_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4706209.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8084..8998)
                     /locus_tag="ACHKNP_RS20725"
                     /old_locus_tag="pgaptmp_004145"
     CDS             complement(8084..8998)
                     /locus_tag="ACHKNP_RS20725"
                     /old_locus_tag="pgaptmp_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU4706210.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDSVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8995..10683)
                     /locus_tag="ACHKNP_RS20730"
                     /old_locus_tag="pgaptmp_004146"
     CDS             complement(8995..10683)
                     /locus_tag="ACHKNP_RS20730"
                     /old_locus_tag="pgaptmp_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HGU4706211.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         2595 a         2678 c         2821 g         2684 t
ORIGIN      
        1 tatgttttta tgaaagcgta attcaggtca atgtcacaat taaccattgt cacaataagg
       61 ttgaacggat attctgagca cggaccacgt aatattcaat acattattta ctgccgggtt
      121 tttcgtgaat caacgtaata tgtcaataat taattccacg ccagtgcgtg ttattgccat
      181 tgtaggatgt gatggttcag gtaaatcgac cctcacggca agcctggtaa atgaactggc
      241 agcaagaatg ccaacagaac gcatttatct cgggcaatcg tccgggcgaa ttggcgaatg
      301 gatttcacag ctccctgtta ttggcgcacc ttttgggcgt tatctgcgaa gtaaagcggc
      361 acatgtgcac gaaaagccct caacaccgcc tggcaatatt actgcgctgg ttatctatct
      421 gctttcctgc tggcgggcgt acaagtttcg caaaatgttg tgtaaaagcc agcaaggctt
      481 tctgctcatc accgaccgct acccgcaggt tgaagtgccg ggttttcgct ttgatggccc
      541 gcaattggca aaaaccacgg gcggtaacgg ttggataaaa atgttaaggc agcgcgagct
      601 gaagctgtac caatggatgg catcttattt gcccgtattg ttgattcgtc ttggcattga
      661 tgaacaaacc gcgtttgcgc gtaaacctga ccaccaactg gcagcgttac aggaaaaaat
      721 cgccgttacg ccgcaactga cattcaatgg cgcaaagatc cttgaactgg atgggcgaca
      781 ccccgccgat gaaattctgc aagcgtcact acgcgcaatt cacgccgcgc tttcctgatc
      841 accaaaatgg ataggggatg atttaacgaa tggatgcact acaaactcaa actgttaata
      901 gtacaaccgc gccgcagccc aactacattc cggggctgat tgcggtggtc gggtgtgatg
      961 gcaccggtaa atccacactg accaccgacc tggtgaaatc gctgcaacaa cactggcaaa
     1021 ccgagcggcg ctatctgggg ctgctctccg gcgaagacgg cgacaaaatc aaacgattgc
     1081 cgttggttgg cgtctggctg gaacggcgac tggcggccaa atcctcgaaa acccaaagca
     1141 tgaaaaccaa atctccggcg ctatgggcgg cggtgattat gtactgcttc tcgctgcgaa
     1201 gaatggcgaa tctacgcaag gttcagcgac tggcgcaaag tggcgttctg gtggtcagcg
     1261 atcgcttccc gcaggctgaa atttcgggct tttattatga tggaccgggg attggcgtcg
     1321 aacgtgcgac cgggaaaatc agcatgtttc tggcgcagcg cgaacggcgt ttataccaac
     1381 aaatggcgca atatcgcccg gaattaatta ttcgcctggg cattgatatt gagactgcca
     1441 tctcccgcaa gcctgaccat gactatgccg agctgcagga caaaatcggc gtcatgtcga
     1501 cgattggcta taacggcaca aaaattcttg agattgattc cagagcgccc tacagcgaag
     1561 tgctggaaca ggcacagaag gcggtttctc tggttgccat cgtttctgac cgccgaagtt
     1621 taacttaagg ctggcgggat actttttact atcaacgata aggtcaggct gaattggcgg
     1681 gttttaacat caaacattgg tttgcagatg gcgcgtttcg caccattatt cgcaatagcg
     1741 cctggttagg ctccagtaat gtcgtgagcg ccttgctggg tctgttggcg ctctcgtgtg
     1801 ccggtaaagg gatgacgccc gccatgtttg gcgtactggt gattgtgcaa tcgtacgcca
     1861 agtcgatcag cgattttatt aagtttcaga catggcaact ggtggttcag tacggaacgc
     1921 cagccttgac caacaataat ccgcagcaat ttcgcaatgt cgtctcattt tccttctcgc
     1981 tggatatcgt cagcggcgcg gtggcgattg tcggcggcat tgccttactg ccattccttt
     2041 cccattcatt aggtctggat gaccaaagct tttggctggc ggcgctgtat tgcacgctca
     2101 ttccttcaat ggcttcctcc acgccgaccg gcattctgcg tgcggtagat cgcttcgatt
     2161 taattgctgt acagcaggcg acgaaacctt ttctgcgcgc agcggggagc gtcgtagcct
     2221 ggtattttga ctttggtttt gcgggttttg ttattgcctg gtacgtgtcg aatctggttg
     2281 gcggcaccat gtactggtgg tttgcagcgc gcgaattacg ccgcagaaat atccataatg
     2341 ccttcaaatt gaatctgttt gagtctgccc gacacatcaa aggcgcgtgg agttttgtct
     2401 ggtcaaccaa cattgcccac tccatctggt cggcgcgtaa ctcgtgcagc acagtgctgg
     2461 tggggatcgt gttaggaccc gctgccgccg ggttatttaa aatcgccatg acattcttcg
     2521 acgccgccgg aacgccagcg ggtttgctgg gtaaaagttt ttacccagag gtgatgcgtt
     2581 tagatccgcg caccaccaga ccgtggttgt taggtgtgaa gtccggttta ctggcgggcg
     2641 gaatcggtat tctggtggcg ctcgcggtgt ttatcgtcgg caagccgctc atttcgctgg
     2701 tgtttggcgt taagtatctc gaagcgtatg acctgattca ggtgatgctg ggcgcaattg
     2761 tgatttcgat gctgggcttc cctcaggagt cattgctatt gatggcggga aaacagcgcg
     2821 cttttctcgt ggcgcaaacc atcgcctcaa tcggctacat cgtattgctg ttcatgttct
     2881 gtcatctgtt tggtgtgctg ggagcagcgt ttgcttactt cggtgggcaa tgtctggacg
     2941 tggcgctctc gttaattcca accttaaagg cattttttca gcgccattcc ttgctttata
     3001 acgcagccgg agagaaatcc tgatgatgag aaaatatttc ccgcttgagg cgagcgagcg
     3061 tctatttgtt gcgattgaag aagatgatgt cgttgatgca caggttagtt tgccgccgac
     3121 tattgcacta agttgtacca ccgaaatcat tcatgataat tacgcgttat gcctgcaatt
     3181 ctggctcaat ggcgttgatc gtcaggaatt actgcgttta gttcgcaaac aagccaaagg
     3241 cgatgagctg actgctgatg agcgcaaaca atttaaatac atgcgagcgc gctacaagca
     3301 cctgcgtttt gcgcaacggc tgtatttaaa gaagcatcag gccggatttt tattcggcaa
     3361 aacgaccgtt tttctggggc gttttcagga cggctttcgt aacggtaaga aaaatattgt
     3421 gtcgtattac ggcaacctgt tacggattta tttaagctct ccggtctggt cgttggtgaa
     3481 ctattcatta cgccatagcc agttagaaag cgtaagcagt tttattgcct atcggcaaaa
     3541 gcaaatgcat accctgaaag agattattgc caaaccgcga ctaaccggca gggaatttca
     3601 tgatgtgcga aaaatcatca gccaacaggt ttcttattac gacaccttaa gatcgcttga
     3661 tcctgaaaat aaagaggcgt tacagatttc ccgtttcctc gccgccatta atggcctgat
     3721 gggagataaa cacgacgaca tggtggcgga tgatatggaa aaccgccagt cgtatgatgc
     3781 accgttggcg ctggatagcg atattcgtca gcgcttagag ctgctgattt cgcgttttcc
     3841 gctatagccg gagcaatgat gtcatctcgt ttgattattg cgttgattat catgctctta
     3901 gccccgggtg tgcaggcaca caatttcgtc actggcaaaa cggttacccc tgtttatatc
     3961 caggaaggcg gcgagctgct tctgaatagc gacgacgaaa ttcactacca aaagtggaac
     4021 agcacacaat tagcaggaaa agtccgcatt attcagtata tcgccgggcg taagtcggcg
     4081 aagaaaaaga actcactgtt aattaaagcg gttgaagcgg caaactttcc gcaggatcgt
     4141 tttcagccca ccaccattgt gaacaccgat gatgccatct ttggtactgg ctattttgtt
     4201 gttggcaaaa tcgaaaaaaa taaacgccgc tatccgtggg cgcaattcat tattgacggc
     4261 aatggacagg gacgcgttgc ctggcgcttg ccggagcaga gttcaaccat tctggtgctg
     4321 aataaagccg ggcaaattca gtgggcaaag gacggcagcc tgacgccgga agaggtagat
     4381 catgtcattg cgctggcgca gaaactgatc aatgaataaa aaattcaaag gattgagggc
     4441 gttagtgaga tgaagctggt tgaacactac atcatgcgtg gtacgcgccg tctggtgctg
     4501 attatcgtcg gttttctgat ctttattttc gccagctact ccgcacagcg ttatctgacc
     4561 gaagcggcaa atgggacatt agctcttgat gttgtgctgg atatcgtttt ctacaaagtg
     4621 ctgattgcac tggagatgtt gttacctgtt ggtctgtatg tgtcagttgg cgtgacgcta
     4681 gggcagatgt ataccgactc ggaaattacc gctatctctg cggcgggggg cagtccggga
     4741 cgcttgtaca aagccgttct ttatctggcg ataccgctaa gtatttttgt cacccttctg
     4801 tcgatgtatg gtcgtccgtg ggcttatacg cagatttatc aactggagca gcagtcgcag
     4861 tcggagctgg atgttcgcca gttgcgggca aagaaattta acactaacga taacggacga
     4921 atgatccttt cgcagacggt tgatcaggat aataatcgcc tgactgacgc gctgatttat
     4981 acttctactg ccaatcgaac ccgcattttc cgcgcccgtt cggttgatgt ggttgaccca
     5041 tcacccgaga aaccgaccgt tatgttgcac aacggcactg cctatcttct cgatcatcag
     5101 gggcgtgacg acaacgaaca gatctaccgt aatctgcaat tacatctgaa tccgctggat
     5161 caaagcccta acgtcaaacg caaagcaaaa tcggtcacgg agctggcgcg ctccgccttt
     5221 cctgccgatc atgccgaact gcaatggcga caaagccgtg gcctgacagc attgttgatg
     5281 gcgctgctgg ccatttcatt aagtcgggta aaaccgcggc aagggcgatt ttcaacatta
     5341 ttgccactga cgttgctgtt tattgccatt ttttatggcg gcgacgtctg ccgtacgctg
     5401 gtggctaacg gcgcgattcc cctcattcct ggtttgtggt tagtacccgg actcatgcta
     5461 atgggcctgc tgatgctggt cgcacgcgac ttctctttgc tgcagaaatt ttcccgatga
     5521 atgttttcag tcgctattta atccgtcatc tctttctcgg ttttgccgcc gccgcagggc
     5581 tattgctgcc gctttttacc accttcaacc tgattaacga actggatgat gtcagcccgg
     5641 gcggttatcg ctggactcag gcggtgctgg tggtgctaat gaccttaccg cgcacactgg
     5701 tcgaactttc gccgtttatc gcgttattgg gtggtattgt tggactgggg cagttatcga
     5761 aaaacagtga gcttaccgcc attcgcagca cggggttttc tatcttccgt attgcactgg
     5821 tggtgctggt tgccgggata ttgtggactg tttcgttagg cgcgatagat gagtgggtgg
     5881 cgtcgccatt gcagcaacag gcgttgcaaa tcaaatcgac cgccaccgcg ttgggggagg
     5941 acgatgacat taccggcaat atgctgtggg ccaggcgtgg taatgaattt gtgacggtga
     6001 aatcgctgaa cgagcagggc cagccggtgg gcgtggagat atttcattat cgcgacgatc
     6061 tctcgcttga atcctacatt tatgcacgca gtgccaccat taaagacgac aaaacgtggg
     6121 tcctgcatgg tgtgaatcat aaaaaatggc tgaatggcaa agaaacgctg gaaacattag
     6181 ataatcttgc ctggcaatcg gccttcacca gtatgaatct tgaagagtta tcaatgccgg
     6241 ggaatacttt ttctgtccgt cagcttaatc attacatcca ttatttgcag gaaaccgggc
     6301 aacccagcag cgaataccgc cttgcactgt gggaaaaact ggggcaaccc atcctgaccc
     6361 tggcgatgat tttgctggct gtgccgttta cctttactgc cccgcgttcg cctgggatgg
     6421 gtagccgtct cgctgtaggc gtcatcgtgg gcttactcac ctggatcagc tatcaaatca
     6481 tggttaatct gggattgcta tttgcgttaa gcgcacctgt taccgcgctc ggtttaccgg
     6541 tagcgtttgt gttggtggcg ttgagcctgg tgtattggta tgacagacaa cattaaaccc
     6601 gccgtggcgc aaacaccacg gcgggctcac attactgctt cagagtagca aatgcctgaa
     6661 tgatggcatt gatttgctca cgactgtggg cagcatttac gctacagcgc agcagcgtga
     6721 tgcctgcagg cgcggcaggc ggcaggataa gattcacata cacgcccagc gagataagct
     6781 cgcgccagat gcgtaaacct tcctctttcg aaccgattat tactggcact accgggctaa
     6841 tatgtgaact taactcataa cccagttttt gcagaccgtc atacagatgg ttggcgttat
     6901 ccatcagctt ttgccgcagc tctggttgtg tggcaatagt tcttaacgac gagcgcacgg
     6961 tcgcaatgca ggacggagaa ggagaggcgg taaaaatata cgggcggctg ctgtagcgca
     7021 gcacttccat cgcttccgaa ccgacagcaa aaccgccaat cgaggccaga cttttactga
     7081 aagtaccaac gataatatcc acatcatctt caacgccgac agcttcagcc agtccgcgcc
     7141 ccgttgcgcc taacacgcca aacgaatgcg cttcatcgac aatcaaataa ccaccgagac
     7201 gacgtttgat atcaacaatt tccgccagcg gcgcaacgtc accgagcatg ctgtagatgc
     7261 cctcaacgat gataatcgcc tctttcgccc gttcaccgag gcgaaccatg cgccgttcca
     7321 gatctttagc atcgttatgg cggaaacgaa taatttccgc gccaccgaga gaacaggcgt
     7381 cataaatact ggcgtggctg tcggcatcca gtaacaccac ggcgttatgg tcggcaagtg
     7441 cgctgatgac gcctaaatta gcggtataac cggttgaaaa aacgatagcg gtagggcgat
     7501 tgaaaaactc ggcaatctct ttctctaacg ccaggtgcgg cgcataactg ccgtttgcca
     7561 tacgagaacc ggtggtgccg gtgccttggg ccgctaacgc tgcctgacct tcgctgatgg
     7621 catcatgatt aaaggttaaa ccgagataat tattggtccc ggccagaatc acttttttat
     7681 tgccgatccg gccttcggtg gcggaataca cttcatcaat acaggtgcca aacggtgtta
     7741 acccgttgtc actaaattgc agtcgctcgc cagcaaggcg tgcatattta tcgtatagcc
     7801 ccattattta ttctccagcc atggaagtag agtttccatt aattgcgcag gggttttgac
     7861 atccagcaga atattaatag gaatggaaat atcgaatctg tcttccagca tcattaagag
     7921 atccatcact ttgatcgact ccaggccaag atcgttgacg agatcgctgt cgggcttaat
     7981 ttccacaccg ttttcgacca aatcctgtaa acaggaaagg atgtaatcca ttactatttc
     8041 acgatttacc atagaaacgt taacacacct ttaaacaaga caatcaaaat agcccgttgc
     8101 gtaatgcgtg ttccaggcta atcccgggaa accaattaat atcttcagaa atacggttat
     8161 tacttgccga ccagtcggcg tgggttaatt cacgaatttt cgagcgggtc agcataggtt
     8221 ctttacctgc caggcgactc aacgcggtac tgatatccgc aaggcaggtg agtaccggca
     8281 gaggaatacc aaccattcgc acggaaccgc aacggacgtt ggcggcaagt tgctgtatgc
     8341 gttgccagtc atagccgcca gcgacgctat cgcataattc ataggtttgc gtctgtacag
     8401 tttcggcgct taaccactga cccactgctt gcgcaaaatc ggtgacgtgc aggaaagaga
     8461 gctgtgtatc tggtgcacca agtcgtggca gcaggccgcg cagcatccag tcaaacagcg
     8521 gttttaactc tttatcgccg ggaccataca cggctgtcgg gcgaaaaacg cccagcgtaa
     8581 tttcgtcagc cattgcagtc agccgttgtt cggcgatgtg tttggaattt gcgtaccagg
     8641 agagctcggg atggcgcgcc gccagcgaag agataaacag aaaacgttgg caaaagcagc
     8701 tctcttttgc tgcttgcatc aggcgcaggc tgccgtcaac gttacagcgg gtgaaaatct
     8761 cttctttgtg cccgcgcact tgcccggcgc aatggaccac tgcgctggct ccggcaacca
     8821 gcttgctaag tgaatgagta tcttccagcg aaccgcgcac ccaggtgaga ttatcattga
     8881 cgtgagcgcg ggcagtacgc gtcaatgcgc gaacgtgaaa gccgcgggcg agcaggttat
     8941 cgataatata tttaccgata aacccggtag cgcccgtcac cgcgacagtt tgattcatgc
     9001 cagggattcc tgcacattaa gactggcagc ataagccttc tgataacgtt ttttcgcttc
     9061 cgcacgggca ggcttgccgg aggacgttcg gggaatactg tggggcggca acagatcgat
     9121 agccgcggtc acgccaaatt cgctttggat ccgtgccgcc agcgcgtgga taagctgccc
     9181 gcgacgttct tcgtcgctga tccgacactg gatctgcaaa atgatttttt cctgggcggt
     9241 aacaaaagca atcgcatcgc cagaatgaat ttccggttct tgttccgcaa tatattcaat
     9301 atcctgcggc cagatattac ggccacgaat aataatcaga tctttaatgc gtccggtgac
     9361 atacagataa ccgtccagca gataaccgag gtcgccggtg tctaaccagc ccgtcgcggc
     9421 aatctcgtct tgcgaaacct ggtcgccaaa gtaaccgctc atcaggctgg ggccggagat
     9481 gcaaatatgg cctaccacac gttccgcgac cggcatacct gcttcattgc ggatttcaat
     9541 gccatgttcc ggcaacgctt tgccgcagtt gacgaaagtc gatacggcgc gtgtctctgc
     9601 acccggcgcg acggctttac cctgatattc gaggatgtcg cgatccactt cgttaaccac
     9661 aaccccggag gcttcatcag agaagctgac agccagcgca ttttctgcca gtccgtagca
     9721 cggcatgaaa gttttattgt caaagttaac ctgacggaaa cattcagcga attgatggag
     9781 ttgttctgcg gagatgggtt ctgcaccaat accagcgacg cgccagcagg aaagatccag
     9841 ttcagcgaga tctttttcat tcacgcggcg ctggcacaat tcatagccaa acggcggcgc
     9901 aacggaaacg gtgccgcgat ttttactgat caatttaagc cattgcagag gacgcatggc
     9961 aaaatcctga gtgcgcaaat aatctactga aagctgcgtg gcgacggggg gcaggagaaa
    10021 gccgaccagt cccatatcat ggtagaaagg cagccaggag acgcagcggt cgccagggcg
    10081 taatttaatg ccgtcgtggc ttatagcacg tagattagcc atcacttcgc gatgggtgat
    10141 aatgacgcca cggggaaaac gggtgctgcc ggaggtgtac tggaggtagg cgatatcgtt
    10201 cggaactgga cgctggagcg caacatcggc ttccggtaat gccttaaacc aggcgtggct
    10261 taaaacatgt aattcggggt tgttatgcgt cgcggcattg accagtggca accactcatc
    10321 accagtgata atggctgcgg gctggcaact tgccagtaaa ccctggagtt tggcgctcca
    10381 ggaatcccgc tgaccgacgc ccattggaat cgccaacggg acggcgacta agccagcata
    10441 ctgacaggca aaaaaagcct ctacaaaccc gctactcgtt tcggcaatca gcgccacgcg
    10501 ttcgcctttt ttcaggttca gcgataacaa ccgcttcgca ccagcttcgg cacgcgtttt
    10561 taatgtctga tattccagtt gatcttcaag ttggcaacgt ctgtcataaa aattcattcc
    10621 ggcgctactc agagcggcgt agtccaaagc atcaaccagc gttggaaaat cggcatagcg
    10681 cataggtagg gaatgcgtaa agattttatt agacatatac accacgaaaa aattggatat
    10741 caaaaataaa atgacaataa tgcggtggat tatatagg
//
LOCUS       DAZFWL020000149        10752 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFWL020000149 DAZFWL020000000
VERSION     DAZFWL020000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.149"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..571)
                     /gene="yncG"
                     /locus_tag="ACHKNP_RS20735"
                     /old_locus_tag="pgaptmp_004147"
     CDS             complement(<1..571)
                     /gene="yncG"
                     /locus_tag="ACHKNP_RS20735"
                     /old_locus_tag="pgaptmp_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HGU4706212.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVC"
     gene            837..2336
                     /gene="ansP"
                     /locus_tag="ACHKNP_RS20740"
                     /old_locus_tag="pgaptmp_004148"
     CDS             837..2336
                     /gene="ansP"
                     /locus_tag="ACHKNP_RS20740"
                     /old_locus_tag="pgaptmp_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HGU4706213.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2451..3512)
                     /gene="yncE"
                     /locus_tag="ACHKNP_RS20745"
                     /old_locus_tag="pgaptmp_004149"
     CDS             complement(2451..3512)
                     /gene="yncE"
                     /locus_tag="ACHKNP_RS20745"
                     /old_locus_tag="pgaptmp_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HGU4706214.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3754..5856
                     /gene="pqqU"
                     /locus_tag="ACHKNP_RS20750"
                     /old_locus_tag="pgaptmp_004150"
     CDS             3754..5856
                     /gene="pqqU"
                     /locus_tag="ACHKNP_RS20750"
                     /old_locus_tag="pgaptmp_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HGU4706215.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5892..6557)
                     /gene="mcbR"
                     /locus_tag="ACHKNP_RS20755"
                     /old_locus_tag="pgaptmp_004151"
     CDS             complement(5892..6557)
                     /gene="mcbR"
                     /locus_tag="ACHKNP_RS20755"
                     /old_locus_tag="pgaptmp_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HGU4706216.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6755..7792)
                     /gene="curA"
                     /locus_tag="ACHKNP_RS20760"
                     /old_locus_tag="pgaptmp_004152"
     CDS             complement(6755..7792)
                     /gene="curA"
                     /locus_tag="ACHKNP_RS20760"
                     /old_locus_tag="pgaptmp_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HGU4706217.1"
                     /translation="MRQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            7973..8491
                     /gene="mddA"
                     /locus_tag="ACHKNP_RS20765"
                     /old_locus_tag="pgaptmp_004153"
     CDS             7973..8491
                     /gene="mddA"
                     /locus_tag="ACHKNP_RS20765"
                     /old_locus_tag="pgaptmp_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HGU4706218.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8488..8937
                     /gene="ydcZ"
                     /locus_tag="ACHKNP_RS20770"
                     /old_locus_tag="pgaptmp_004154"
     CDS             8488..8937
                     /gene="ydcZ"
                     /locus_tag="ACHKNP_RS20770"
                     /old_locus_tag="pgaptmp_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4706219.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8938..9171)
                     /gene="ydcY"
                     /locus_tag="ACHKNP_RS20775"
                     /old_locus_tag="pgaptmp_004155"
     CDS             complement(8938..9171)
                     /gene="ydcY"
                     /locus_tag="ACHKNP_RS20775"
                     /old_locus_tag="pgaptmp_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HGU4706220.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9257..9430)
                     /gene="ortT"
                     /locus_tag="ACHKNP_RS20780"
                     /old_locus_tag="pgaptmp_004156"
     CDS             complement(9257..9430)
                     /gene="ortT"
                     /locus_tag="ACHKNP_RS20780"
                     /old_locus_tag="pgaptmp_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HGU4706221.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9625..9720
                     /gene="yncL"
                     /locus_tag="ACHKNP_RS20785"
                     /old_locus_tag="pgaptmp_004157"
     CDS             9625..9720
                     /gene="yncL"
                     /locus_tag="ACHKNP_RS20785"
                     /old_locus_tag="pgaptmp_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HGU4706222.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(10122..>10752)
                     /locus_tag="ACHKNP_RS20790"
                     /old_locus_tag="pgaptmp_004158"
     CDS             complement(10122..>10752)
                     /locus_tag="ACHKNP_RS20790"
                     /old_locus_tag="pgaptmp_004158"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HGU4706223.1"
                     /translation="DNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIASN
                     VKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGN
                     DVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWW
                     NYNYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDYKKFNLSEVFRGL"
BASE COUNT         2650 a         2605 c         2560 g         2937 t
ORIGIN      
        1 gacatacagc atcagcgatc gcgctgatat tctgcgcatt atcctgaaac cattcatgcc
       61 ccggccccca tgtgcgcatg gtgcagagat aacagtccac cagcgttagt tgctcaccaa
      121 aggcataagg ttcagcggtc aattgtgaat tcagccagat ataaagtgat ttgcgatatt
      181 caattacatt cttctttaac tgttcaggtg cgtcagatgc ccaacgctca gggtaatcgg
      241 cgaaagtgaa tgttggatag acattagcaa ccagccagac caatagccgt tgaaactgct
      301 gacgttcggc gcgcccaacc ggtggcgcaa ggtccgggcg acgatcgaga accataaatg
      361 caatcgccgc tgtctccgtc ataatctcgt cattttccag cgccagtgtc ggaacctgac
      421 acaacgggtt cagggttttc aataactcgc gtgaagctcc ctcgtggtca aaaccactga
      481 catcaacgaa ctgataagga atatcagcca gtgttagcat cagctcactg attgtagagc
      541 cccatccggg tacgccatac accttaatca tatgccctca caatctttta aagattaagt
      601 gtaggacagt acacaggtta accagcaaaa agatttgtca ttttttcgtc agttgtatta
      661 ttccccttca ggattgtaag gggatgtttt gcatcgttta tcgataacgg gtaggatgct
      721 gcgccgatag ttttataccc ataaagaata atggtgataa ctatcatcgc caggatgaat
      781 aaacattgtt catggcaact tatatgactt tttcattaaa gcaatcaggg agagcaatga
      841 gtaaacacga caccgacact tcagatcaac acgccgcgaa acgccgctgg cttaatgccc
      901 acgaagaggg gtatcacaaa gcgatgggca atcgccaggt acagatgatc gccattggcg
      961 gcgcgattgg caccggcttg tttttaggtg caggagcccg actgcaaatg gcgggtccag
     1021 cattggcact ggtttattta atttgtggct tgttttcgtt ttttattctg cgtgcattgg
     1081 gtgagctggt gctacaccgc ccttccagtg gcagttttgt ctcttatgcc cgtgagtttt
     1141 tgggtgagaa agccgcttat gttgctggct ggatgtactt catcaactgg gcgatgacgg
     1201 ggattgttga tattaccgcc gtcgctctgt atatgcatta ctggggtgcg tttggcggcg
     1261 tgccgcagtg ggtctttgcg ctcgctgcac ttaccatcgt tggcaccatg aatatgatcg
     1321 gtgtgaaatg gtttgcggag atggagttct ggtttgcgct tattaaagtg ctcgccattg
     1381 tgaccttttt ggtcgtgggt acagtgttcc tcggtagtgg tcagccgctg gatggcaaca
     1441 ccactggctt tcatttaatt accgataatg gcggcttctt cccccacggt ttgctgcctg
     1501 ctctggtgtt gattcagggc gtagtgtttg cttttgcctc cattgaaatg gtgggtacag
     1561 ctgccggaga atgtaaagat ccgcagacca tggtgcctaa agcgattaac agcgtgattt
     1621 ggcgtattgg cctgttttac gtcggctccg tggtgttgct ggttatgcta ttgccgtgga
     1681 gcgcgtatca ggcggggcaa agtccgttcg tgacgttttt ctctaaactg ggtgtgccat
     1741 atatcggcag cattatgaac attgtggtgc tgaccgctgc cctctccagc ctgaactcag
     1801 gtctgtactg caccggacgt attctgcgct caatggcgat gggcggttcc gcaccgagtt
     1861 ttatggcgaa aatgagtcgt cagcatgtgc cgtatgccgg gattctggcg acactagttg
     1921 tgtatgtcgt cggcgtattc ctcaactatc tggtgccgtc gcgcgtattt gagattgtgt
     1981 tgaacttcgc gtcgctggga atcatcgctt catgggcgtt tatcatcgtg tgccagatgc
     2041 gcctgcgtaa agcaattaaa caaggcaaag cagcggatgt cagttttaaa ctgcctggcg
     2101 cgcccttcac ttcctggctg acattactgt ttttactgag tgtcctggtg ctgatggcgt
     2161 tcgattaccc gaacgggact tatactatcg cggcgctgcc gattatcggt attctgctgg
     2221 ttataggctg gtttggtgtg cgcaaacgcg ttgctgaaat tcacagcact gcgccagtcg
     2281 tcgaggaaga tgaagaaaaa caggaaattg tgtttaagcc tgaaacggcg agctaattgc
     2341 aaaattgccc ggctacgtgc cgggcaaatt tagattactg acgaaggtgt gccgcatgcg
     2401 gcgtgataaa agtcaggcaa gtctctccgt tttcaccctc gccacatcta ttacagcgca
     2461 atacgaatca catcgtccgg ctgggtagct tcctgctgtt tagtggattt ttgtttcaca
     2521 ctgacataca gcgttttgcc atcggcagac agcgccaggc tgtttggatg agtcggcgta
     2581 tcgaacgttt tcaccacttt atagcttttc gcgtcaatca cactgacttt acctgcctga
     2641 cgatgcgtta cgtaggcttc attacgcgct gggttaaaca gcacagccag tgattctggt
     2701 gccgcaacct tcgccagaat attgccatta cgggtatcga ccactaacac ttctgcggct
     2761 ttagaatcgg tgataaatgc acgctgcctg gcggtatcaa ggctaatgtt gataaagaag
     2821 tgctctttgc cgtcatccag cagcttttta cggctgagga ttttattgtc ggcggtgtcg
     2881 atggtaatca attcgccgtc agcgttagtg gtgtaaagac gtttgccttt gctatccagc
     2941 gccagaccgg tactcatttt accggtgttc tggatggcgg ttttcagttt gatattctcg
     3001 ccatcaacga cccaaatcac gctctcttta ccaataccac tgatgtaaac ggtgttcgtg
     3061 gcatcatcag ctaccagctc acgcggttgc agcgggcgca cctcttccgt gcgcttacga
     3121 tcatccagca ccagacggcc tttcacctcg cccgttttgg catctatcgc cgtgaccgcg
     3181 ctgtttacgg tgttaccaaa ccacaacgtc tgagtcgtgt tattgatggt ggcaccaaac
     3241 ggcttgagat cgttatggat cgcctgcgtc acttccagag tgaccggatc aagacgataa
     3301 accacgccac ctttatccag tttgcggctt tgcgaagtgg cgagccacag tgcgttttct
     3361 tgctggctat aagccatttc gtaggcacct ttacctaccg ctttacgcag catttcttct
     3421 gcggcctgcg tactgaatga tgaagcaaca agcaatgaac ctaacagtaa tgaaccacgc
     3481 aggcgcgatg aaaacagatg acgtaaatgc atgacgactc cctttgatga accgatattt
     3541 ttgctgcttc acaatgcttc gctaagcgta atcatcgtta cgctcttgtt ataaatggga
     3601 atggtaatca ttattttctc aaatgtggag catttcttgc cgttacgact acaattttct
     3661 caattactta acgtatcgtg ccgtttcgtt ttccataaat ttacaatttc attaccataa
     3721 cggcgttttc ccattcttaa cctggctttt ttcatgaaga ttttttccgt ccgacagacc
     3781 gttttgcccg cactgcttgt cctttccccc gttgtttttg ccgctgatga acagactatg
     3841 attgtcagtg ccgcaccgca ggtggtttca gaactggata ccccagcagc agtaagcgtg
     3901 gtggatggcg aggagatgcg cctggcaaca ccgcgcatta acttgtccga atcactgacc
     3961 ggcgtgcctg gtttgcaggt acaaaaccgg cagaactatg cgcaagattt acagctgtcg
     4021 attcgcggat ttggctcccg ctccacttac ggtattcgcg gtattcgcct gtatgtggac
     4081 ggtattcccg ccaccatgcc cgacgggcaa gggcaaacat ccaacatcga tttaagcagt
     4141 gtgcaaaatg tggaagtgct gcgtggcccc ttctctgccc tgtatggcaa cgcgtctggt
     4201 ggggtaatga atgtcaccac ccagaccgga caacagccac caaccattga agccagtagt
     4261 tactacggca gttttggcag ctggcgctat gggctgaaag caacgggcgc aacgggagac
     4321 ggcacacagc ctggcgatgt cgattacacc gtctcaacca cgcgttttac gacccacggc
     4381 tatcgtgacc atagtggcgc acagaaaaat ttagctaatg ccaaactggg cgtacgcatt
     4441 gatgaagcca gcaaattaag tctgattttc aatagtgtgg atatcaaagc agatgaccca
     4501 ggtgggctaa ccaaagcaga atggaaggct aatccacaac aagcgcctcg tgcagaacag
     4561 tacgacacgc gaaaaaccat caagcaaact caggctgggt tgcgctatga gcgtagcctg
     4621 agcgcgcaag atgatatgag tgtgatgatg tatgccggag agcgagaaac gacccagtac
     4681 cagtcaatcc cgatggcccc ccaacttaac ccgtcacatg cgggcggcgt gattaccctg
     4741 caacgccatt accaggggat agacagccgc tggacacacc gtggtgaact gggcgttccg
     4801 gtcacgttca ctaccggcct gaactacgaa aacatgagtg aaaaccgcaa gggctacaat
     4861 aacttccgcc tgaatagcgg catgccggag tacgggcaaa aaggtgagtt gcgtcgcgac
     4921 gaacgcaatc tgatgtggaa catcgatccc tatttacaga cgcagtggca gctgagcgaa
     4981 aaactgtcgc tggatgctgg cgtgcgctac agctccgtgt ggtttgattc caacgaccat
     5041 tacgttactc cgggtaacgg cgatgacagc ggtgatgcca gttatcataa atggctacct
     5101 gccggttcgt taaaatatgc aatgaccgat gcctggaata tctatctggc agccgggcga
     5161 ggttttgaaa cgccgacgat taatgagctg tcttatcgtg ctgatgggca aagcggtatg
     5221 aactttggtt taaaaccatc caccaacgat acaattgaga tcggcagtaa aacgcgtatt
     5281 ggtgatgggc tgcttagtct cgcattgttt cagaccgaca ctgatgatga aattgttgtc
     5341 gatagcagta gcggtgggcg tacgacttac aaaaatgccg gaaagacccg tcgtcaaggc
     5401 gctgaactgg catgggatca acgtttcgca ggagattttc gcgtaaacgc gtcctggacc
     5461 tggcttgatg cgacctatcg cagcaatgtt tgcaatgaac aggattgtaa cggtaatcgg
     5521 atgccaggga tcgcccgtaa tatgggcttt gcgtcgatag gttatgtacc ggaagatggt
     5581 tggtatgcag gcacggaagc gcgttatatg ggcgatatta tggcagatga tgaaaatacg
     5641 gcaaaagcgc cgtcttatac tctcgtcggc ttattcaccg ggtataaata caattaccac
     5701 aatttaactg tggatttatt tggtcgtgtc gataatttat tcgataaaga atacgttggt
     5761 tctgtcattg tcaatgagtc aaacgggcga tattacgaac cttcgcccgg acgaaattat
     5821 ggtgtcggca tgaatattgc gtggagattt gagtaattac cttgatgccc ggtatttgcc
     5881 gggcatttac tttaacgatt gtattgctgg tataaaatag caatattttg ttgcataatt
     5941 tcagcaagac aatgtctgca tccctctttg tcttttgctt ttaacgcagc gagtaacaga
     6001 tgatagtttt ctatatgctc ccgtaattcc gctggattaa tcgcttcata gagataatgt
     6061 aaaccaggcc ccatcctgac ccacagttgc tcaatcatct cacacaggat gggcatattt
     6121 gagcgatgat aaatcgctaa gcgaaatagc ctgtttacat taatgatttg ttccatgtcg
     6181 cccttttctt gcgcctgctg taatttctcg agcaattcct ggagttccgc caggtcttgc
     6241 ggggtgaggt tttcaacagc cagagcaact gccattaatt ccagttcgta gcggatccga
     6301 ttgatttcgt ctaattgacg tttcctcact tccggaactg taaatgcttg tgcaggtgcg
     6361 acagaaagcg cattcaccga aaccagacgt aataatgctt cacgcacagg tgtaatactc
     6421 atacctaatt gctccgccag atttttagta atcaggcgtg cgccaggttt taatgcgcca
     6481 atactaagct gatgtttcag gtcgttctca acctgcagag tcaaactgac atgtttcatt
     6541 tttcccattc caggcatttt aattccttcg cgtgttttct gtccaaccct gcctgcacgg
     6601 cagaaggagt tcacctggtg tattaaagtg aataatcata aaccacccac ctttgttgtt
     6661 attagcagca gaacaatgac gtggttagtt aattaaagct gaaaataagc tttttgcctg
     6721 gatttttatg gcggcttacg ccgccgttga aactttaatc atcacccgcg acgcggatca
     6781 ccactttgcc gaagttttta cccttcagca ggccgataaa cgtctgtggc gcattctcta
     6841 aaccatcagt aatatcttcg tggtagtgga ttttatcctc tttcacccat tgccccatct
     6901 ccttctgaaa ctcatggatg cggtgaccat aatcctgagc gataataaac ccttgcaagc
     6961 gaatacgttt tttcagcacc gttgccatca acaggggtaa acgatccgga ccgggtggta
     7021 gctctgtagc gttatagctg ctcactaagc cacaaacggg aatgcgcgca gatgtattaa
     7081 gtaatggtag cactgcatca aataccttac cgcccacgtt ttcataatag atatcaatac
     7141 cttttgggca cgctttcacc agttgttcgg caaaatcatc cgcgtggtga tcaagacaaa
     7201 catcgaagcc cagcacctcg atagcatggc ggcatttttc cgcgccaccg gcaaccccca
     7261 ccaccctgca acctttaagt ttgccgattt gccccaccgt tgctcccaca ggacctgtcg
     7321 ccgcagctac caccaacgtt tcgccctctt taggctgacc gatatccagc agccccatat
     7381 aagcggtaaa gcctggcatc cccagcacac ccagcgacca cgatggattt tgcggatgat
     7441 cgccaagttt caccagatca tcaccactgg atatgtcata gtcctgccat ccactgtagc
     7501 ccagcaccca gtcgccaggc tgataatcag gatgattcga ctccacaaca cggctcaccg
     7561 tgccgccgac catcacgccg ccaatatcaa caggcggtga ataagatggc tcatcgctca
     7621 tacgtccacg catatacggg tccagggaca aataaactgt gcgcagtaac acctgtcctt
     7681 cacccggtgt ggcgacatca tcttcttcaa gacggaaatt ctccggaacc ggtgcgccat
     7741 gtggacgcga ggccagaacc caacgtcgat tacgctgctt ttgttgcctc ataatgctct
     7801 cctgtgcttt tggcatgaat ccatcaatag tagcaggctc acgggattgg ttttgtttgc
     7861 agccgtcaca tcccctctgc aacacgcaat aatttcatcg aggaatccgt tgcttcgggc
     7921 tgttttcgat agtattgata acatcatcat cattatttta tcgaggccag acatgtccat
     7981 ccgttttgcc cgcaaagccg attgtgctgc cattgcggaa atttataacc acgccgtgtt
     8041 gtatacggcg gctatctgga atgaccaaac ggttgatgca gataaccgca ttgcctggtt
     8101 tgaagcgcgg accatagcag gttatccggt gctggtgagt gaagaagatg gcgtagtgac
     8161 gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata ccgtggaaca
     8221 ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat tgttaagccg
     8281 attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga tcgaatcgca
     8341 aaatcaggcc tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg cacaaatgcc
     8401 gcaggtgggc actaaatttg ggcgctggct ggatctgaca tttatgcagt tgcaactcga
     8461 cgaacgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc ttttttgatt
     8521 gccgccggaa ttggtctggt agtccagaac acgctgatgg tacgcatcac ccagacctcc
     8581 tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct ctttgtttct
     8641 attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag cgtgcgctgg
     8701 tggacactta tacccggctt gctgggatcg ttttttgtct tcgccagtat cagcggatat
     8761 cagaatgtgg gagcggcgac aactattgca gtactggtcg ccagccagct aattggcggt
     8821 ctgatgctgg atatattccg cagtcatggc gtaccactac gggcgttgtt cggcccaatt
     8881 tgtggcgcaa ttttacttgt ggttggcgcc tggttggtag ccagacgctc attttaatca
     8941 gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt ctttatgctt
     9001 gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc gatcttcccg
     9061 acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg caatgacgct
     9121 ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca tactttctcc
     9181 ttaaccactt gtgaaattta tcgattcatc caaatgatat aagtctgcgt ggtgccgtca
     9241 agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa ctcatcggcc
     9301 atgttgcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg tgagaaagaa
     9361 accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc atatgttgat
     9421 agagagacat gtacgggcgt ccagccagat gaataaaacg cgcgcaaata tgcgtcattt
     9481 tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca gcaacttgcc
     9541 cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact atacacatag
     9601 gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt actgataaat
     9661 gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg gtttatttga
     9721 tgtagtgatt ttgcaagcag cacgtactgt cagagaaatc aactctctga cagtacgcaa
     9781 gatcttctca tactctccag gtttacaaca ttatccttaa tatttacttc tttttgctgg
     9841 catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa acagcttact
     9901 gattcaaacg gaatcttgtt ttcacatttt tctatcatga atactttata cgcgcgggag
     9961 tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg cgtaaagtat
    10021 atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa ttgcaaaccc
    10081 cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc tctgaatacc
    10141 tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatctga tatcaatcga
    10201 ttcatctctt caacaaaata atttatatca tcacacttgt tattatccgg aagatctgag
    10261 tagttataat tccaccattt tattctcaac agttcatcaa taacatttga atcaaaccta
    10321 aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac atctttggta
    10381 acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc tttcaataca
    10441 acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt ggtaatgaat
    10501 ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa ttcaccgtta
    10561 taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat tatttttaca
    10621 tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg gcaggtcgga
    10681 aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata ttcttcagca
    10741 ataacattat ct
//
LOCUS       DAZFWL020000150        10661 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFWL020000150 DAZFWL020000000
VERSION     DAZFWL020000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.150"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            138..1505
                     /gene="dcuC"
                     /locus_tag="ACHKNP_RS20795"
                     /old_locus_tag="pgaptmp_004159"
     CDS             138..1505
                     /gene="dcuC"
                     /locus_tag="ACHKNP_RS20795"
                     /old_locus_tag="pgaptmp_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU4706224.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVAV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1548..2045)
                     /gene="sspB"
                     /locus_tag="ACHKNP_RS20800"
                     /old_locus_tag="pgaptmp_004160"
     CDS             complement(1548..2045)
                     /gene="sspB"
                     /locus_tag="ACHKNP_RS20800"
                     /old_locus_tag="pgaptmp_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HGU4706225.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2051..2689)
                     /gene="sspA"
                     /locus_tag="ACHKNP_RS20805"
                     /old_locus_tag="pgaptmp_004161"
     CDS             complement(2051..2689)
                     /gene="sspA"
                     /locus_tag="ACHKNP_RS20805"
                     /old_locus_tag="pgaptmp_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HGU4706226.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3084..3476)
                     /gene="rpsI"
                     /locus_tag="ACHKNP_RS20810"
                     /old_locus_tag="pgaptmp_004162"
     CDS             complement(3084..3476)
                     /gene="rpsI"
                     /locus_tag="ACHKNP_RS20810"
                     /old_locus_tag="pgaptmp_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGU4706227.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3492..3920)
                     /gene="rplM"
                     /locus_tag="ACHKNP_RS20815"
                     /old_locus_tag="pgaptmp_004163"
     CDS             complement(3492..3920)
                     /gene="rplM"
                     /locus_tag="ACHKNP_RS20815"
                     /old_locus_tag="pgaptmp_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGU4706228.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4139..5266)
                     /gene="zapE"
                     /locus_tag="ACHKNP_RS20820"
                     /old_locus_tag="pgaptmp_004164"
     CDS             complement(4139..5266)
                     /gene="zapE"
                     /locus_tag="ACHKNP_RS20820"
                     /old_locus_tag="pgaptmp_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HGU4706229.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5460..5858
                     /gene="zapG"
                     /locus_tag="ACHKNP_RS20825"
                     /old_locus_tag="pgaptmp_004165"
     CDS             5460..5858
                     /gene="zapG"
                     /locus_tag="ACHKNP_RS20825"
                     /old_locus_tag="pgaptmp_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HGU4706230.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6012..7379
                     /gene="degQ"
                     /locus_tag="ACHKNP_RS20830"
                     /old_locus_tag="pgaptmp_004166"
     CDS             6012..7379
                     /gene="degQ"
                     /locus_tag="ACHKNP_RS20830"
                     /old_locus_tag="pgaptmp_004166"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HGU4706231.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7469..8536
                     /gene="degS"
                     /locus_tag="ACHKNP_RS20835"
                     /old_locus_tag="pgaptmp_004167"
     CDS             7469..8536
                     /gene="degS"
                     /locus_tag="ACHKNP_RS20835"
                     /old_locus_tag="pgaptmp_004167"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HGU4706232.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8599..9537)
                     /gene="mdh"
                     /locus_tag="ACHKNP_RS20840"
                     /old_locus_tag="pgaptmp_004168"
     CDS             complement(8599..9537)
                     /gene="mdh"
                     /locus_tag="ACHKNP_RS20840"
                     /old_locus_tag="pgaptmp_004168"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU4706233.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            9972..10442
                     /gene="argR"
                     /locus_tag="ACHKNP_RS20845"
                     /old_locus_tag="pgaptmp_004169"
     CDS             9972..10442
                     /gene="argR"
                     /locus_tag="ACHKNP_RS20845"
                     /old_locus_tag="pgaptmp_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HGU4706234.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2637 a         2697 c         2638 g         2689 t
ORIGIN      
        1 tttaatgaaa aatcacctca cctacaatta aaaacacgac atccgcacca taaatagact
       61 tgcaaaaaat ataacatcat tgttttcaat ctgccgttta tgggattgac cgttttcttt
      121 tgacacggag ttcaacaatg ttcggcataa ttatatctgt catcgtatta attacgatgg
      181 gctatttgat cctgaaaaac tacaaacctc aggtggtgct ggctgccgca ggtatcttcc
      241 tgatgatgtg cggtgtctgg ttagggttcg gtggtgtact cgatcccgcc aaaagcagcg
      301 gctacttgat cgtcgatatt tataatgaaa tcctgcgcat gctgtccaac cgcattgccg
      361 gattggggct gtcgattatg gcagtgggcg gttatgcccg ctacatggag cgcacaggag
      421 ccagtcgcgc gatggtgagc ttgctaagcc gcccgttaaa actcattcgc tcgccgtata
      481 ttattctgtc ggcaacttac gtcatcggcc aaatcatggc gcagtttatt accagcgcct
      541 ccggcctggg tatgttgctg atggtcacct tatttccgac gctggtgagt ctgggagtaa
      601 gtcgcctctc tgcggtggcg gttatcgcaa ccacgatgtc cattgagtgg gggattctgg
      661 aaacgaactc catttttgca gcccaggtcg cgggaatgaa aattgccact tacttcttcc
      721 actaccagct tccggtcgcc tcttgcgtca ttatctcggt ggcgatctcc cactttttcg
      781 tgcaacgcgc ttttgacaaa aaagataaaa atatcaatca cgaacaggca gagctaaaag
      841 ctctcgataa tgtcccgccg ctctattacg ccattttacc tgtgatgccg ttaatcttga
      901 tgctcggctc gctgttcctc gcccacatcg ggctgatgca gtcagaactg catctggtgg
      961 cggtgatgtt actgagtttg acggtgacga tgtttgttga gttcttccgc aagcataact
     1021 tgcgcgaaac aatggacgat gtgcaggcgt tttttgacgg catgggtacg cagtttgcca
     1081 acgtggtaac gctggtggtc gcgggtgaaa tatttgcgaa aggcttaacg acgattggca
     1141 ctgttgatgc ggttatcagg ggtgcggagc attctggtct gggcggtatt ggcgtgatga
     1201 ttattatggc gctagtcatt gccatttgtg ccattgtgat gggctctggc aatgcgccgt
     1261 ttatgtcatt tgccagtctt attccgaata tcgcagccgg actacatgta ccagcggttg
     1321 taatgattat gccgatgcat tttgccacga cgctagcgcg cgcggtttcg ccgattactg
     1381 cggtggtggt cgttacgtca ggaattgcag gcgtttcgcc ttttgcggtg gtgaagcgaa
     1441 cagcgatccc catggcagtc ggtttcgtgg tgaatatgat tgccacaatc acgctatttt
     1501 attaagccat taaaagacaa aacaggccac cagggcctgt ttttgtatta cttcacaacg
     1561 cgtaatgccg gtcgaccacc gcgtggtggc tgcggaggtt catcgtcagg atgagtgtca
     1621 tcatcgtgat ctggcttgtc gccatcaata accgacataa cggtttcgtt gtctgccgat
     1681 gcctcttcat cattcatgat gctggtatct tcatcgtagg cagcttcagg ctcaaacatc
     1741 gtgcctgcgc cattttcacg ggcgtagata gccagcacgg cagccagcgg cacagaaacc
     1801 tgacgcggaa tgccgccaaa gcgcgcgtta aagcgtacct catcattcgc cagttccaga
     1861 ttgccgacag cacgcggcgc aatgttgagt acgatttgcc cgtcacgcgc atattccata
     1921 ggaacctgca cgccagggag cgtcacatcc accaccaagt gcggcgtgag ctggttatcc
     1981 agcaaccact catagaatgc acgcagcaga tagggacgac gtggtgttag ctgtgacaaa
     2041 tccatacaga ttaactccgg cccagacgca tttcacgttc tgcttcagtt aaagaagcaa
     2101 ggaaagagtc acgctcaaag acgcgggtca tatagccttt cagctctttc gcacccgggc
     2161 cgctgaactc gatgcccagt tgcggcagac gccacagcag cggagcaaga tagcaatcga
     2221 ccaggctgaa ctcatcgctc aggaagtacg gcttctgacc gaagaccggc gcaatcgcca
     2281 gcagttcttc gcgcagttgc ttacgtgcgg catctgcttc agaagctgaa ccgttgatga
     2341 tggtgttcat cagcgtgtac cagtcttttt cgatgcgatg catgtacaga cggctttcac
     2401 cgcgagctac cgggtaaaca ggcatcagtg gcggatgcgg gaaacgctca tccagatatt
     2461 ccataatgat gcgagattcc cacagggtca gctcacgatc caccagggtc ggaacgctct
     2521 gattcgggtt gaggtcaatc agatcctgag gcggattgtc cttttccacg tgttcgatct
     2581 cgaaacttac acctttctca gccagcacaa tgcggacctg atggctatag atgtcagtag
     2641 gaccggaaaa cagcgtcatt accgaacgtt tgttggcagc gacagccatg aaaacctcca
     2701 ggtatagtca gaatttttac tgctaccagc caccaggtgg ccagtcagaa gttgtgttac
     2761 ccagtaagga acgactctct ttgttcgaaa atcaaacaaa aaatgagcaa tacccgacat
     2821 ttgggcagaa aattggatga tagtttacca gattttgcga cctttgtggt gagtcgatgc
     2881 cggaaatggg gaaaaagaga tgcgctttag tctgaaatag ttgacttagt cccttattgg
     2941 cgatgtggtt tttgttttgc ctgtctgtca gacggtagca aaaagcaact ttccaatttt
     3001 tacgctgatt cagaatttaa ccataaaaaa acccgccgaa gcgggttttt tcgcaaattg
     3061 ttttctgccg gagcagaagc caattaacgt ttggagaact gcggacgacg acgtgcttta
     3121 cgcagaccga ctttcttacg ttcaacctga cgagcgtcac gagtaacgaa gccagcttta
     3181 cgcagttcag aacgcaggga ctcgtcgtat tccatcagag cgcgggtgat accgtgacgg
     3241 atcgcaccag cctgaccaga gataccacca cctttaacgg tgatgtacag gtccagtttc
     3301 tcaaccatgt cgaccagttc cagcggctga cgaactacca tgcgagcagt ttcacgaccg
     3361 aagtactgtt ccagagaacg ttggttgatt acgattttac cgttgcccgg tttgatgaaa
     3421 acgcgagctg cggaactttt gcggcgacca gtgccgtagt attgattttc agccattgcc
     3481 tataatcccg attagatgtc aagaacttgc ggttgctgtg ccgcgtggtt gtgctcgtta
     3541 cccgcgtaaa ctttcagttt acggaacata gcacgaccca gcgggccttt tggcaacatg
     3601 cctttaaccg cgatttcaat cacacgctca ggacggcgag caatcatctc ttcaaaggtc
     3661 gcttgtttga taccaccgat gtggccggtg tggtgatagt acactttgtc agtacgcttg
     3721 ttgccggtta cagcaacttt gtcagcgttc agaacgatga tgtaatcacc ggtatctacg
     3781 tgcggagtgt attccgcttt gtgcttaccg cgcaggcgac gagccagttc agtagccaga
     3841 cggcccagag ttttaccggt cgcgtcaaca acataccagt cgcgttttac ggtttctggt
     3901 ttagctgtaa aagttttcat taaaagctta cccaataaat agttacacgt tggtgaacac
     3961 ccaaacgtct tcaattgttg aggttcacac gacaaagtcc ggcaaaccta ccccttcgaa
     4021 tagcctatgc cagcacacaa aaagttttgg gaaaaaaact ttcttgtaac gtggggtcgc
     4081 aggattatag agaagtcggg gtcaaagatc gacccctttt tgtgatttgt gacaggtttt
     4141 aacccgccaa atgctcgcgc ttcagatact cttcgctttg catctcttgc agacgtgaca
     4201 ggcaacgctg gaactcaaac ttcagccgat cgccctgata aatttcatac agcggcactt
     4261 ctgcactcac cactaatttg acatggcgct cgtaaaactc atccaccagc gcaataaagc
     4321 gccgcgcttc gctctccatc aaccgcgtca taactggtac atcaaacaac atgaccgtat
     4381 gaaagagacg tgagagcgca atatagtcat gctgactgcg ggcgtcgacg cacagcgtag
     4441 taaaagagac cgccagcgtc tggttctcga cgcccattgt cgccaatggc cgatggttga
     4501 tttctaacgt cggtgaattt tctcgtttcg cccccgccag cgccaaccat agtttatcca
     4561 tctgcgcccg ggtttcatcg ttgagtggcg aaagccacag atgcgcctga gtgagtgtac
     4621 gcagtcgata atcaacacca gcgtccacgt tcattacatc acaatgctgt ttaatggcat
     4681 cgattgcagg cagaaaacgc gcacgttgca ggccatttcg ataaagttca tccggcggaa
     4741 tatttgacgt cgctaccagg gtaatgccgc gggcgaacag ggctttcatc agaccgccaa
     4801 gtagcatggc gtcggtaata tcagaaacaa aaaattcgtc aaaacagagc acgtcagttt
     4861 cggctttaaa gcgatcggca ataatttcca gcggatcggt ctgcccctgt aaggcagtta
     4921 gctcttcgtg cacccgcagc ataaaacggt gaaagtgcag gcgctgtttc cgctctcccg
     4981 gcaggctttg atagaaaagg tccatcagcc aggttttccc gcgtcctaca ccgccccaca
     5041 tatataagcc acgcactggc gtatgctttg tgtcttcgcg tttaccccac agcttaccga
     5101 cccgcgccat tagcccactc gtcctggggg ctggtggcct gctattgatg agttcttgat
     5161 aaataatttc caggcggctg acggcctctt tttgaacgtc gtcgggttga tggctgcctt
     5221 cattgagcgc cttcaggtat tgcgatgttg gggtaacgct ttgcatgatc ttattgttat
     5281 tccttgaata atcggtgcgc cgttgttcac ggttgacgaa aaaaaggccg ttctacacta
     5341 cgcgatatgc agtcgggatt ccacttctgt ggaattaacg gttatagtgg cataatcagc
     5401 cgcaggcatg gagcctgaag ccaacaccct acggaaacaa aagacaacgg gagatgttca
     5461 tgacctggga atatgcgcta attgggttag tcgtcggcat cattattggt gctgtggcca
     5521 tgcgttttgg taatcgtaaa ctacgccagc aacaggcgtt gcagtacgaa ctggaaaaga
     5581 ataaagctga actggacgag tatcgcgaag agctggttag ccactttgcc cgcagcgcgg
     5641 aattactgga taccatggcg cacgactatc gccagctgta tcagcacatg gcgaaaagct
     5701 ccagcagcct gctgccggaa ctgtctgctg aagcaaaccc gttccgtaat cgtctggcag
     5761 agtctgaagc cagcaacgat caggcaccgg tgcagatgcc tcgcgactat tctgaaggcg
     5821 catccggcct gctgcgtact ggcgcgaagc gcgactaatt tatttttcgg gcgcagccat
     5881 tgcgccctcc tcttctctcc ctccccgact atcatttaat ctggtgtctc attgttagcc
     5941 gtctgaaaat tcaataacat caaactgttt tgaatctctt ttcttatcat tcaggtacga
     6001 gagcaggaat aatgaaaaaa caaacccagc tgttgagtgc attagcgtta agtgtcgggt
     6061 taactctctc ggcgtcattt caggccgtcg cgtcgattcc aggccaggtt gccgatcagg
     6121 cccctctccc cagtctggct ccaatgctgg aaaaagtgct tccggcagtg gtgagcgtac
     6181 gggtggaagg aacggccagt cagggacaga aaatcccgga agaattcaaa aagttttttg
     6241 gtgatgattt accggatcaa cctgcacaac ccttcgaagg tttaggttcc ggtgtcatca
     6301 tcaacgccag taaaggctat gtgctgacca acaaccatgt gattaatcag gcacagaaaa
     6361 tcagtattca gctcaatgat gggcgcgagt ttgatgcaaa actgattggt agcgatgacc
     6421 agagcgatat cgccctgtta caaattcaaa acccgagcaa attaacgcaa atcgctattg
     6481 ccgactccga taaattgcgc gtcggtgatt ttgccgtagc ggtcggtaac ccatttggcc
     6541 ttgggcaaac cgccacctct ggcattgttt ccgcattagg ccgcagcggg ttgaatcttg
     6601 aaggtctgga aaactttatc cagacagatg cttccattaa ccgcggtaac tccggcggtg
     6661 cactattaaa ccttaacggt gagttaattg gcatcaacac tgcaatcctt gcgcctggcg
     6721 gcgggagcgt cgggattgga tttgccatcc ccagtaatat ggcgcgaaca ctggcgcagc
     6781 agcttatcga ctttggtgaa atcaaacgcg gtttgttagg catcaaaggc accgagatga
     6841 gtgccgatat cgccaaagcc ttcaaccttg acgtgcagcg tggcgcgttt gtcagcgaag
     6901 tgttgccagg ttctggctcg gcaaaagcgg gcgtcaaagc gggcgatatt attaccagcc
     6961 tcaacggcaa accgctgaat agctttgctg agttgcgctc tcgtatcgcg actacagagc
     7021 cgggcacgaa agtgaagctt ggcctgctgc gtaacggcaa accactggaa gtagaagtga
     7081 cgcttgatac cagcacctct tcgtcggcca gcgctgaaat gatcacgcca gcgctggaag
     7141 gtgcaacgtt gagcgatggt cagctaaaag atggcggcaa aggtattaag atcgatgaag
     7201 ttgtcaaagg aagcccagct gctcaggctg gcttgcaaaa agacgatgtg atcattggcg
     7261 tcaaccgcga tcgggtgaac tcgattgctg aaatgcgtaa agtgctggcg gcaaaaccgg
     7321 ccatcatcgc cctgcaaatt gtacgcggta atgaaagcat ctatctgctg atgcgttaat
     7381 gtcgtaaacc gggcatcagg cttacgtgtg atgtccggtt aactcgtggt atgctgctgc
     7441 cgttcccttt tttaatgacg cctccatcat gtttgtgaag ctcttacgtt ccgttgcgat
     7501 tggattaatt gtcggcgcta ttctgctggt tgccatgcct tcgctgcgca gccttaaccc
     7561 gctttccact ccgcaatttg acagtaccga tgagacgcct gccagttata atctggcggt
     7621 tcgccgcgcc gcgccagcgg tggttaacgt ttacaaccgt ggtttgaaca ccaactctca
     7681 caaccagctt gagatccgca ccctgggatc cggtgtaatc atggatcaac gcggttatat
     7741 catcaccaat aaacacgtca tcaacgacgc cgatcagatc atcgtcgcct tacaggatgg
     7801 acgtgtattt gaagcattgc tggtgggatc tgactctcta accgatctgg cggtacttaa
     7861 aattaatgcc actggcggtt tacctaccat tccgatcaat gcacgtcgcg taccgcacat
     7921 tggcgacgtg gtactggcga tcggtaaccc gtacaacctc gggcagacca ttacccaggg
     7981 gattattagt gctacgggtc gaatcggtct gaacccgacc gggcggcaaa acttcctgca
     8041 aaccgatgct tccattaacc acggtaactc tggcggcgcg ctggtgaact cgctgggaga
     8101 actgatgggc attaacacgc tgtcgtttga taagagtaac gatggcgaaa cgccggaagg
     8161 tatcggcttt gcgattcctt tccagttagc aaccaaaatt atggataagc tgatccgcga
     8221 tggtcgcgtg atccgcggct acattggtat cggcggacgc gagatcgcac cactgcacgc
     8281 gcagggcggt ggtatagatc aactgcaagg gatcgtggtt aatgaagtgt cacctgacgg
     8341 cccggcggcg aatgcgggta ttcaggtcaa cgatctgatt atttcggtgg ataacaaacc
     8401 ggccatctct gctctggaga cgatggatca ggtggcagaa attcgccctg gttcggtgat
     8461 cccggttgta gtgatgcgtg atgataagca gttaacgctg caggtcacca ttcaggaata
     8521 tccggcaacc aattaagtcg agtgctcaaa acaaaaaacc ggagtctgtg ctccggtttt
     8581 ttattattcg ctaattaatt acttattaac gaactcttcg cccagggcga tatctttctt
     8641 cagcgtatcc agcatacctt ccagtgcgct ctgttcaaat gcgctcaggg taccgataga
     8701 tttacgctct tccacgccgt ttttacccag cagcagcggt tgagagaaga aacgggcgta
     8761 ctgaccgtcg ccttcaacgt aggcacattc gacaacgcct tgttcgccct gcagtgcacg
     8821 aaccagagac agaccaaaac gtgcagctgc ctggcccata gacagggttg cagacccacc
     8881 accggctttc gcttcaacca cttcagtacc tgcgttctgg atacgtttgg tcagatcagc
     8941 cacttcctgc tcggtaaaac taacgccagg aacctgtgac agcagcggca gaatggtaac
     9001 accagagtga ccgccaataa ccggcacttc aacttcgcct ggctgtttgc ctttcagttc
     9061 cgcaacaaaa gtgttggaac gaatgatatc cagcgtggta acgccgaaca gtttgttttt
     9121 gtcataaaca ccggcttttt tcagcacttc agcagcaatc gcaactgtgg tgttaaccgg
     9181 gttagtgata ataccaatgc acgctttcgg gcaggttttc gcaacttgct gtaccaggtt
     9241 tttcacgatg ccggcgttaa cgttaaacag gtcggaacga tccatacccg gtttacgcgc
     9301 tacacctgca gagataagaa cgacatctgc gccttccagc gccggagtcg catcttcacc
     9361 agaaaaacct ttgattttca cagcagtagg gatatggctc agatcgacag ccacaccggg
     9421 agtcactgga gcgatatcat acagagagag ttctgaacct gaaggcagtt gggtttttaa
     9481 cagtagtgca agcgcctggc caataccgcc agcagcgccg aggactgcga ctttcatcct
     9541 aaactcctta ttatattgat aaactaagat atgttgctcc gctgccgcga ccttaatcca
     9601 caaaattgcc gtttacaatg accacatctc aagaatgtgt agtcacgcaa gtttagcgtt
     9661 tatgcattta attgccgtaa tcaggaacct aacttacgta attaacagtc gcttatgcat
     9721 tagcgcaaca ttccaacagg tggtgacaat ataccctacc gttcagccaa aacaacatca
     9781 atttgataac aattaattta cttttaaaca gaatttgcat gccgtgacgc aggcatgttt
     9841 ctcaataacg aaatttgata aaatcccgct ctttcataac attatttcag ccttcttcag
     9901 ggctgactgt ttgcataaaa attcatctgt atgcacaata atgttgtatc aaccaccata
     9961 tcgggtgact tatgcgaagc tcggctaagc aagaagaact agttaaagca tttaaagcat
    10021 tacttaaaga agagaaattt agctcccagg gcgaaatcgt cgccgcgttg caggagcaag
    10081 gctttgacaa tattaatcag tctaaagtct cgcggatgtt gaccaagttt ggtgctgtac
    10141 gtacacgcaa tgccaaaatg gaaatggttt actgcctgcc agctgaactg ggtgtaccaa
    10201 ctacctccag tccattgaag aatctggtgc tggatatcga ctacaacgat gcagttgtcg
    10261 tgattcatac cagccctggc gcggcgcagt taattgctcg cctgctggac tcactgggca
    10321 aagcagaagg tattctgggc accatcgctg gcgatgacac catctttacc acccctgcta
    10381 acggtttcac cgtcaaagac ctgtacgaag caattttaga gctgttcgac caggagcttt
    10441 aatctctgcc ccgtcgcttc tggcgtcggg gaaaatgttg cttatccctc tcattcccct
    10501 tctttctcct gcgatacatt taacgaatag tgcgttttac cgcgacacat cattcacaaa
    10561 atgaatacat acggtaaaaa aagcacatta tgcaaaattc attatctaat tgaaaatact
    10621 ataattaata ataaataacc gtgtttttta tacttttttg t
//
LOCUS       DAZFWL020000151        10500 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFWL020000151 DAZFWL020000000
VERSION     DAZFWL020000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.151"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..149)
                     /locus_tag="ACHKNP_RS20850"
                     /old_locus_tag="pgaptmp_004170"
     CDS             complement(<1..149)
                     /locus_tag="ACHKNP_RS20850"
                     /old_locus_tag="pgaptmp_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HGU4706235.1"
                     /translation="MLSYLMAIHFVLFGDSTNLKNFWKYEVIRRKRMDIWRLLREKKQ
                     RNRNFL"
     gene            complement(306..794)
                     /locus_tag="ACHKNP_RS20855"
                     /old_locus_tag="pgaptmp_004171"
     CDS             complement(306..794)
                     /locus_tag="ACHKNP_RS20855"
                     /old_locus_tag="pgaptmp_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4706236.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSATSSVKG
                     LL"
     gene            complement(807..3614)
                     /locus_tag="ACHKNP_RS20860"
                     /old_locus_tag="pgaptmp_004172"
     CDS             complement(807..3614)
                     /locus_tag="ACHKNP_RS20860"
                     /old_locus_tag="pgaptmp_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4706237.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RAREWYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAVGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLGSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SRVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLNPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPAASRNREPAVSRFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            complement(3601..3756)
                     /locus_tag="ACHKNP_RS20865"
                     /old_locus_tag="pgaptmp_004173"
     CDS             complement(3601..3756)
                     /locus_tag="ACHKNP_RS20865"
                     /old_locus_tag="pgaptmp_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HGU4706238.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            complement(3765..4130)
                     /locus_tag="ACHKNP_RS20870"
                     /old_locus_tag="pgaptmp_004174"
     CDS             complement(3765..4130)
                     /locus_tag="ACHKNP_RS20870"
                     /old_locus_tag="pgaptmp_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4706239.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            complement(4185..4697)
                     /locus_tag="ACHKNP_RS20875"
                     /old_locus_tag="pgaptmp_004175"
     CDS             complement(4185..4697)
                     /locus_tag="ACHKNP_RS20875"
                     /old_locus_tag="pgaptmp_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HGU4706240.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            complement(4697..5881)
                     /locus_tag="ACHKNP_RS20880"
                     /old_locus_tag="pgaptmp_004176"
     CDS             complement(4697..5881)
                     /locus_tag="ACHKNP_RS20880"
                     /old_locus_tag="pgaptmp_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGU4706241.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHDCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGVRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            6039..7139
                     /locus_tag="ACHKNP_RS20885"
                     /old_locus_tag="pgaptmp_004177"
     CDS             6039..7139
                     /locus_tag="ACHKNP_RS20885"
                     /old_locus_tag="pgaptmp_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HGU4706242.1"
                     /translation="MTDTTMQLLSQSTDPVKMPDFDILAEGETLSGVAERLMSLSLTD
                     NRGFEADLLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSSNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPKKEKSSSREGDYMEGAEGNVFVLR
                     KTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVTGFKSDIDNQDW
                     IIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            7539..8678
                     /locus_tag="ACHKNP_RS20890"
                     /old_locus_tag="pgaptmp_004178"
     CDS             7539..8678
                     /locus_tag="ACHKNP_RS20890"
                     /old_locus_tag="pgaptmp_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014606071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HGU4706243.1"
                     /translation="MGNNKVKLGRNDKCWCNSGLKYKKCHLGRDRETPVSKAEALKFS
                     KSLSQRKACYAPASMHEDCDKRIVQAHTISKSSSLKAIADSSNHVMGVIMNLASLMRN
                     NGKWLPEKIGINQASTFTGFCAVHDRILFSCLENEKFTGTDEQCFALMFRSLSKEIYA
                     KEGGVLSSDFAKNADKGKLQVEQYYIQKLIDQHQSGLNAAIIELNNLKTSLDRILLSR
                     QFSEIESVIFTFAEPLPIVVSSILSPERDFDGTKIQDLSDLTVSAEQVCFNAFSCGGK
                     GYVVFSWLRTSTIIRRFIRTLIKIPANRMFNTLLYFFFTKAENTYSSPEWWDRLSDKQ
                     RENIGNMIMSGVEFFGRPTSQVDHSVDYNAAALEEITCSNNEICS"
     gene            8968..9228
                     /locus_tag="ACHKNP_RS20895"
                     /old_locus_tag="pgaptmp_004179"
     CDS             8968..9228
                     /locus_tag="ACHKNP_RS20895"
                     /old_locus_tag="pgaptmp_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HGU4706244.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHELPASHRGNNQMSLPLPQN"
     gene            9419..9559
                     /locus_tag="ACHKNP_RS20900"
                     /old_locus_tag="pgaptmp_004180"
     CDS             9419..9559
                     /locus_tag="ACHKNP_RS20900"
                     /old_locus_tag="pgaptmp_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4706245.1"
                     /translation="MSQKSLTAITFCVTVILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(9748..10029)
                     /locus_tag="ACHKNP_RS20905"
                     /old_locus_tag="pgaptmp_004181"
     CDS             complement(9748..10029)
                     /locus_tag="ACHKNP_RS20905"
                     /old_locus_tag="pgaptmp_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706246.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRNGGYDVVCYEGDVTDEMIRQWKQENAE"
BASE COUNT         2685 a         2812 c         2496 g         2507 t
ORIGIN      
        1 aggaaattcc ggttacgctg ttttttctct cttaaaagcc tccagatatc catacgtttc
       61 cgccgaatta cttcatattt ccagaagttt tttaaattag tagagtcccc aaataaaaca
      121 aagtgaattg ccattaagta agacagcacg ataatctcct taattattat ttcagaccac
      181 acatattata aggttaagag attataaaat cctgttgttt gttattcaaa aacaattttc
      241 tgagaaggac atacaacagc aagtcgccag tcaccttcat caggaaattg gcgacatacg
      301 ttaaatcaga gcagcccctt aactgagctg gtcgcgctat taagggatga tgtcacctta
      361 tctttgaagc cggacaacat atcgctgaac gatgaggatt gcaggcgctc ccgcaaatcc
      421 tcatcacagc gttcaagagt cagtgaaaat tctatctttt tcgccttacc gtagcgatca
      481 aactcggagc gggtcgtatt cgtttcggtc aggacataca tgccgtaaat ctgcccgacg
      541 ccatcaatca gaggccaggg ccgtcctgta tacgcctgcg tggtcagcag agacagcgac
      601 acttcgccac ctgtaatttc aggataaagc acaccagaaa gaacgatgcg atcatcacct
      661 gcaccgatat actgccagct tgctgaacgg ttaacgcgtt catttttcac atgccgccag
      721 cttttgtttt gctgtaactg ctgatgcggc agcgtgcgca gctcaaaaac aaacatgcca
      781 tagatcatca tcatggccat gactcctcaa tctttatcgt aaaaactgcc acgcccggca
      841 cgggcgcgcc gttccatttc tgccctgacc atttcaccga ccagtttcgc cagttcgcgg
      901 ggattctgcg taacaacgtt atgcagatga acatgaattt caccgccaaa tctggagaca
      961 gcaggctccc gattacggga agctgcagga actgatgcca ctggcgatcg tatggcctcc
     1021 gccaccgggc gggagctggc cgcaacaaca gggaccagcg ccggaggcag cggagccggg
     1081 actacgggtg tgatattaat tgcgggggca ggcttactga cctgcgcaat cttccgctcc
     1141 tgccactccc cacgaacagc aagtgcgcgg ggcaggttct taaagacaat atcgccaggg
     1201 ccaatgcgtt ttttcgtctc atcaaccagc ttacctgtgt tatcagcaat tttgctgagt
     1261 ctgcgtagcg tcccggtatt gctgtctgtg agcggtttgt tgtctttggg tttatcacct
     1321 ccggtgccat tgccattttc cacaggcttc ggcggattga ttttcgccag gtccccctga
     1381 agtaaggcaa ccttatcctg aagaatggcc gcacgctgtg cgtcttcgat tttcttgcgc
     1441 gccctttccg cttcatccgg aaggacgcca agtttttcaa gtatccacgc cagcgtatcc
     1501 agtagcattt ttgcaggtgt cagaacaagt tgtaacgcac cgccaagaac gttaccgaat
     1561 atctcgccag cactggtaca tttatccagc gtttccttgc tggactccat cggtgacagc
     1621 agcgatttaa accagttaaa cacctggctg atcccgcttc cgattgcgtc aaaaacaggg
     1681 ccaaaccgtt caaaggtttc acgcaacggg ttcagccttt ccataatccc gctgaacacc
     1741 ccggcaaaaa atgccctgat gggatcccag tatttccaga taaggacggc agctccggca
     1801 agcgcagcca cgataagacc aaccggactg aacagcgccc cgatagcgcc tcccagtaaa
     1861 gaaacggaac ccgtcaccat tccccatagt gctggcagga ccctgacagc attcattgat
     1921 ccggtcagga gagaaaaacc aagacgcagt tttgccagcg gaccagcaag cacaccaata
     1981 gccagcgaca acgagccaac cgttgcagtc attgccagca acgcaccgcc tgctatcagt
     2041 agctggcgcg tcagtgccgg atgggcctgc gccagcgccg tcacccttga taccacccgc
     2101 gtgagccact gcgtgacaga acgcagcgga ccgtcaatca gatctgcaat gcggatgcgc
     2161 aacccttccc atgcactgcc gagtgatttc agatcgccgt caaggttgtt ggccataacc
     2221 tttgctgtgc gttcagcctc accgcgcgcg ccttcaagtt cttttctcag tttgggtaag
     2281 gaaccgtcac ccactgcatc aacgagcgcc ataaacgatg tgaaagcctc ttctccggca
     2341 atgtccttaa agaacgatac ccggtcaact tccccgtatt tgcgggtggc tttataaagg
     2401 tcggccagca catcctccat cgggcgcatt ttacccccgg catccgagac ggacacgccc
     2461 agctctttca gagcttctgc cgccgccttt ggcggtgatg ccagacgagc caggctggca
     2521 cgcattgccg tcccggcatc actccctctg atgcccatat tcgccagcac accagccatc
     2581 gctgcggcct gctccagcga tattcccagc ttacccgcca ccggacctgc atatttcatg
     2641 gtttcgccca gtgcgcgaag gtcagtgttg gtacgggtaa acgctgcggt gagtgtgtca
     2701 ccgacccggt ccatctggtc agcagaaagg ccgaactgcg tcaggatatt tgagccaata
     2761 tctgccgtct cgccgagatc cataccgcca gccgttgcca tgctcagcac tccgggaagc
     2821 gcagcctgaa tggcctgcgg tgtgaagcct gccattgcaa gaaatgcctg cccactggcg
     2881 gcatcgcctg cggtgaactg cgtttcagag ccaagtttta acgcctgctc acgcagcgcc
     2941 ttaaactgcg ggctgttctg gtcgattcgc gtcagcgcct gaacgcggga catctctttg
     3001 ccgaacccga tcgcgggctg caaaaaacgc ccggcagcat agccgcccgc cgctgccgca
     3061 ccaattgcca gcgcaccacc tgttttcagt tttcccgcgg tttcctgcgc gcgcgaatac
     3121 cactcacgcg cccgcgttac acgcgcaagc gcctgccgtt cgcgttcaag ctggttgttg
     3181 tactgttcgg tgcgtctgat ggcctgctgg atggtgttat cgctgcctgt cagggaaatg
     3241 ccgtggcgtt tcagctctcc gccaagctcc cgcattttct gaatttcccg tgtgcgcgat
     3301 tcattcaggc gttcaagccg ggtgcttaac tgctgcatca gcttttgttg tttttcgctg
     3361 agcactgtac ccgtgcgttg taactgatta agggcgttaa gctggcgtcg tgctttcacg
     3421 atacccgcat ccgctttact gacagcgtcg cgggcgcgct caaatgaacg agcctgacgc
     3481 tcgagatttt taatcgcccc ctgcgttcgc tggatggagt caccaaactg ccccatcagg
     3541 cggcgggcgt tttcggcagg ccgggtcagc ctgtcaacgg cgctgaaagc gacccggata
     3601 tcaagagtct tcattgtctg cattcccgct gcgaagtgcc gcccgctcac gccagctaac
     3661 cacttcgccg ggcgtcatca tgaagatttc ggcgggcgac cagttaaaaa taacggcaat
     3721 atctgccaca aagtcttcta tgtgctcaaa gcacacaacc gtgatcaggc ttccgtcgcc
     3781 tgttcgttct tcccgccaga gtccgcaccg ctcaaaaaat ttacggcaac cacacataac
     3841 tgaataaagt cacgggatgc catttttttg atcgtcactt catccagtcg cggtgatgtc
     3901 acgcgtgaca gcagcgtaaa catggattcc gctttcagat tcagcacatc agacagcgac
     3961 aaatctcgca gagatccagc ctgctcaata gccccggtga tctccacata cgtgattttt
     4021 tcgccgcctc gctcaattgg ttgggtaagt tttacaccac gctcactggt ttctttcaca
     4081 gtgtcagcaa ctaccgtgtt ttcggtatcg atgtttttcg tctctttcat caggaaactc
     4141 ctttcagtca gaggcgacgc actgcgccgc ctgcatatta cttatcagcc aagcccgagc
     4201 gcggaacgga tgcgatcggg cacaatgtcc ttgccgtcct tccggtaaat gaagttcagc
     4261 aggtcaatct cccacaacgg gcgatcgtta acactcagct tgtagtaggt gtttttaatg
     4321 gcgtaagtgt gtgatgtggc ttcgccctgt ttggcttccc ccatatcaat ttccgtcaca
     4381 cgtccgcgca tttcgacttc atacaggtcg ctttctgcat cggtgtaata ttcacccgca
     4441 aaacgcagca gcgtgccgtc aatcgtgccg ccatacttaa ggaacagctc acgaactgcg
     4501 ccccccatga caaagctcgc atcaagcgcg gagtcgtcca gaccgagatc aatacttacc
     4561 gcacccatca tgccaccacc ccggtagctg tcggttttgc gcgtcagctt aggcagagtg
     4621 acggacgtca ccttacccac ttcgttttca ccatccacaa acagcgtaaa aaagcgaaga
     4681 tgttttggta cagccatcag gcacctccca gcaccgcaaa tgcgggttca aagtattcat
     4741 cagtaaacgt ctggtaaagc tccatgtctt ccagtggcgg aacgggcgta tatttgtagc
     4801 gaatacgcac acgcccctga cgtaaatccg tggtgctgtt atccaccacg tcataccagc
     4861 actccgcgcc aatcagtttc ccggcagtaa ccagcgaatc cagttttgcc ctgatggcac
     4921 tgataacatc cttcacgttc gcaggcgtca gtggactgtc gatggtttca aactgcgctt
     4981 ccgcaattga atcagccagc acctgtgcgg ttcgggtata cacctcaaag atgtaggcgt
     5041 tcgtttccgg tgtgcggttg ccccagaagc ggaccccgtt gcgacgaata atggtcgtga
     5101 tttctttgtt gttgaggctg ttggcatcac tgtcttcggc ctgcaacgac cagaaaacat
     5161 gcctggacat ccccagcaca tttttaacag gaacgttgga cagcgatttg tgccagccct
     5221 gctcatggtc aatgtacgca cgaaggccgc acgcataggc aggcgcgggg aacgtttcgt
     5281 ttttgccact tttcgggttg taggcgatga agtccggcca taagagcatc acctcacgtt
     5341 cgttgaattt ctggcggtag gtaattgcct cagccatcgt gttacagtca tgacatgagg
     5401 catacacaaa cgcgcgcagt ttacccgcaa tcacgcacag ggattttgtc accgcctccg
     5461 tgtccagctc cggcgcggcc agaatacgcg gacggtatcc gatgctttca tcctgctctg
     5521 caacaagcag cgcatacatc cccgtatagc tgccgtcaga ttcagaacca ccgataacca
     5581 gttgatcctg cgtcttaccg ccttcttctt tgtgttcagc cacgcgaacg acgatcactt
     5641 ttgtgctcac ctggtctgca atagccttaa gcgcacgata aagcgtcccc gtagtcccgc
     5701 attttcccag cacgtcattg acgcgggtca gcagtgtggg cttgttcagc gggaacagct
     5761 ccgcgtccgc atcatccgcc gttgccacga taccgataac actggaatca acatcattaa
     5821 tcgctgttac caggtcggta ctttccgtaa cacgggcacc atgaaaacga gtttcactca
     5881 tagcttcagc cccttgtatc cgttaaatga ttcggcaaca atcatcaccc accacgcgcg
     5941 taatctcacc cctgcgccgt tctcccgcca cggcgacaac aaaaagcagt aaccccctat
     6001 gcacgcacat gcgaccatgc cgcacaggga gggaacagat gaccgatacc accatgcaat
     6061 tgctcagtca gagcacagac cccgtgaaaa tgccggattt tgatattctc gcggaggggg
     6121 aaacactgtc cggcgtggca gagcgcctga tgagcctgtc actgaccgac aaccggggat
     6181 ttgaagcgga cctgctcacc atcacgctgg atgatgcgga tggtcagttg cagctaccgc
     6241 cacggggcgc gcgcctgacg gttctcattg gctggaaagg agaaccgctg acagaaaaag
     6301 gcacttacat tgttgatgaa atcgctcacg aaggaccgcc ggacaggctg actgtttcag
     6361 ccagaagcgc agattttcgg gatgaattta acgttaaacg tgaggtgtcc tggcatgatg
     6421 tgaccgttga gcgcgtggta tccgccatcg ctcatcggta cggtctgaaa ccacaaatca
     6481 gcgaaatgct gatggatatt gaaatcgacc acgccgacca gaccgaagaa agcgacatgt
     6541 ccttccttac gcgcatggcg gaaatgctgg gcgcaatcac tacggtaaaa agcagtaatc
     6601 tgttattcat catgccaggc ggtggcgtga acgcacaggg ccagccgttg ccatcgttcg
     6661 ccatcacgcg cagcagtggc gatcgccatc agttccgcat tgctgaccgc gaagcgtata
     6721 cgggggtacg tgcttactgg cttgatctta attacgggaa aaagaaaaaa gtcagcgtga
     6781 aacgccgcaa accgaaaaag gagaaaagca gcagccgtga aggtgactat atggaaggtg
     6841 cggaaggcaa cgtgtttgtg ttacgcaaga cttatcagaa cgagcaggca gcaagacgtg
     6901 cagcggcggc aaagtggcag cagctacaac gcggagccgc atcattctcc atcacgctgg
     6961 cgcgtggacg cgcagaactc tatcccgaaa tgcatggcac ggtgacaggc ttcaaaagtg
     7021 acatcgataa tcaggactgg attattgcaa aagccgagca caccattgat aacagcggct
     7081 ttaccacaca gcttgagctt gaagcaaaaa tcccggaatg gatagcagaa acagaatgag
     7141 caaattagat gtattgactc aaaccagaac tgacacactc acagcacagt gccagaccca
     7201 atctggcagc ccgctttgtg cctagagcag gtgttgctta tgtaacagtt aatccacgga
     7261 tagtggtcat gaatgctggt tgaagttgta ttgccatgag tggtcaatta gggactcact
     7321 tattatcgcg tgctcaccat tgatgggtaa gatcatgcat ttcggctact gtacttcgcg
     7381 aatcctccgc acacaggcaa cacctgattt aagaacagag ggagatgatg acgcaaggaa
     7441 gatttattat cgtgcatgtg gcagatgatc ttttagctgc cggagtgata aattacacct
     7501 attttcgata agtgtttttg ttatagaaca ttaatttcgt gggaaacaat aaagtgaaat
     7561 taggaagaaa tgataagtgc tggtgtaatt caggacttaa gtataaaaag tgccatcttg
     7621 gtcgagatcg tgaaactccg gtatccaaag ccgaggcact gaaattttca aagtctctct
     7681 cgcagagaaa ggcatgttat gcgccagcat ccatgcacga agattgtgat aaacgcatcg
     7741 ttcaggcgca tacgatatct aagagcagta gccttaaggc gatcgccgac tccagcaatc
     7801 acgtcatggg ggtgataatg aacttagcaa gcctgatgag aaacaacgga aaatggcttc
     7861 ccgagaagat cggtatcaat caggcatcca cttttacagg attttgtgca gttcatgaca
     7921 gaatcttatt ttcatgccta gagaacgaaa aatttacagg cacggacgag cagtgctttg
     7981 ccttaatgtt tcgttcattg tctaaagaaa tttatgccaa agaaggggga gtgctatctt
     8041 ctgattttgc taaaaatgcc gataaaggaa agctacaagt tgaacagtat tatattcaga
     8101 aattaatcga ccagcaccaa agtggcttaa atgcagcaat aattgaatta aataatctta
     8161 aaactagcct cgaccgcatc ctacttagca ggcaattttc agaaattgaa agtgttatct
     8221 tcacttttgc agagccttta ccaattgtag tttcgtcaat tctttcaccg gagagggatt
     8281 tcgatgggac caaaattcag gatttgagcg atcttacagt gtcggcggaa caagtatgtt
     8341 tcaatgcttt ttcctgcgga ggtaagggat atgtagtgtt cagttggctg agaacttcaa
     8401 caattatcag acgttttata cggacactaa ttaagatccc agctaacagg atgttcaata
     8461 cccttctgta ttttttcttc acaaaggccg aaaatacata ttcatcccct gagtggtggg
     8521 acaggctcag tgataaacag cgagagaata tcggcaacat gattatgtca ggggttgaat
     8581 tttttggcag acctacatca caggttgatc attccgttga ttataacgcc gctgcacttg
     8641 aagagataac atgcagcaac aacgaaattt gcagctgaat gtttacatgc tgttagaaaa
     8701 agcgggctga acaatcattt cgggattgta acagataaga tggcactgcc cgcttgcttc
     8761 ccgatatgca cctcagccaa taaacttatg taagcaaccg atttccattt atgcggaggt
     8821 tatcaaagtt gttggagttg cgaacttccg ctcttcgcac aaagcgtacc gctaggtttg
     8881 attgtattct ggctgcgcaa actgtcagtt ggagtctgag ctattacaac ttagaatagc
     8941 ggcagcacca cgttaaggga ggtcgctatg ttccgttgtc cgctttgtgg cgcatctgcc
     9001 cgaatccgca ccagtcgtcc ggaaaacgat tcaaacactg tgcggcaaaa gtattaccag
     9061 tgtaacaacc tggaatgcgg cgtatgcttc tcaacactgg aagctttcca taaattcaca
     9121 tcaaaacacg cctccggcgt tcactcttcg gaaggtatcc cgtggcatga gctgccagct
     9181 tcacacaggg gaaacaatca gatgagtttg cctttacctc agaattaaca ggcagaattg
     9241 ccggagtaac aaaaaaacga tagattaccc gcgggtgcct ttcggctgat ggtcggagga
     9301 atacccgaag gccggatgtg gaaaggcccc ggaaaacatt tctgtttaac cgaggcccta
     9361 acatatctac cttaagcaag tgataggtta gcgcctctcc aacaaaggag caagcgctat
     9421 gtcgcaaaaa tcgcttacag ccatcacgtt ctgcgtgacg gtaatcctca tcatctggat
     9481 gctacacggt tcgctgtgtg aaatacggat gagcttctgg ggagcggagt ttgcggcgtt
     9541 cttacagtgt aagcagtaag gaaaccgcga cgggggagca atcccccgtc aatcggttgc
     9601 taaggttggc tgaagtggca ccctatctca cagacatgaa caacaaaccc gcagcgtaag
     9661 aactgcgggt tttctttttg gaacaatcac tagctagtgt accgttaacg gcacataatg
     9721 ctcgcacaac aaacctgtta aaagagatta ctcagcattc tcctgcttcc attgccggat
     9781 catttcatcg gtaacatcac cctcataaca taccacgtca taccctccat tacggctata
     9841 tgcactgcgt ccaccacatc tactaccatt ccttgcatga ttataaggac acgcacaatt
     9901 gcctggataa gattcaatgg attctttaat tatttctttt ttgatctgag catcagactt
     9961 tcctgttgct gcatatccac taaaagatac taaacaaagg cagattgcca ttaataaacc
    10021 acatcgcata ccgatacact ctattactaa ccaatcatag cccacatcat aaaatcgaga
    10081 tgaggagtac aataccttta acaagaaggt gtcataacga taatatgtcg tgcaattcat
    10141 caaaaaaaag atctatatca aagagataga actcttatca gtgcttcaga catcggcatt
    10201 cgaggcagca gaaaaacagt aaatctgacg aatttgcctt ttcccaagat gttgtcagat
    10261 taaaaagata gattagcgca ggtacctgtt ggtggattgc aggaggaaaa atgaagtcga
    10321 gtgaaaaaat cccagaaaaa tttttgttta acagaggtcc tggttcacta tgcgaaatac
    10381 ggaggagctg cagaagaatt ctgcttcatc cttagagttt aaacagcacg tgcactacca
    10441 ttgtttctgc atatcgccac accatcgcca ctctaccgcc attgcacaaa atacaaatac
//
LOCUS       DAZFWL020000152        10460 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFWL020000152 DAZFWL020000000
VERSION     DAZFWL020000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.152"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            158..733
                     /gene="ssuE"
                     /locus_tag="ACHKNP_RS20910"
                     /old_locus_tag="pgaptmp_004182"
     CDS             158..733
                     /gene="ssuE"
                     /locus_tag="ACHKNP_RS20910"
                     /old_locus_tag="pgaptmp_004182"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGU4706247.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            726..1685
                     /gene="ssuA"
                     /locus_tag="ACHKNP_RS20915"
                     /old_locus_tag="pgaptmp_004183"
     CDS             726..1685
                     /gene="ssuA"
                     /locus_tag="ACHKNP_RS20915"
                     /old_locus_tag="pgaptmp_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HGU4706248.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGAVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAKVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1682..2827
                     /gene="ssuD"
                     /locus_tag="ACHKNP_RS20920"
                     /old_locus_tag="pgaptmp_004184"
     CDS             1682..2827
                     /gene="ssuD"
                     /locus_tag="ACHKNP_RS20920"
                     /old_locus_tag="pgaptmp_004184"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HGU4706249.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2838..3629
                     /gene="ssuC"
                     /locus_tag="ACHKNP_RS20925"
                     /old_locus_tag="pgaptmp_004185"
     CDS             2838..3629
                     /gene="ssuC"
                     /locus_tag="ACHKNP_RS20925"
                     /old_locus_tag="pgaptmp_004185"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HGU4706250.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3626..4393
                     /gene="ssuB"
                     /locus_tag="ACHKNP_RS20930"
                     /old_locus_tag="pgaptmp_004186"
     CDS             3626..4393
                     /gene="ssuB"
                     /locus_tag="ACHKNP_RS20930"
                     /old_locus_tag="pgaptmp_004186"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU4706251.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4600..7212)
                     /gene="pepN"
                     /locus_tag="ACHKNP_RS20935"
                     /old_locus_tag="pgaptmp_004187"
     CDS             complement(4600..7212)
                     /gene="pepN"
                     /locus_tag="ACHKNP_RS20935"
                     /old_locus_tag="pgaptmp_004187"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HGU4706252.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7478..8680
                     /gene="pncB"
                     /locus_tag="ACHKNP_RS20940"
                     /old_locus_tag="pgaptmp_004188"
     CDS             7478..8680
                     /gene="pncB"
                     /locus_tag="ACHKNP_RS20940"
                     /old_locus_tag="pgaptmp_004188"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGU4706253.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8849..10249
                     /gene="asnS"
                     /locus_tag="ACHKNP_RS20945"
                     /old_locus_tag="pgaptmp_004189"
     CDS             8849..10249
                     /gene="asnS"
                     /locus_tag="ACHKNP_RS20945"
                     /old_locus_tag="pgaptmp_004189"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGU4706254.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2371 a         2698 c         2801 g         2590 t
ORIGIN      
        1 caaataaata tgcgtgatat ataaagccct ttctttagtt tattttcaga aaaagataca
       61 caaaatttat atttggcatt ttcttgtctc tccgacagac tgaccgaatt gttatcaatg
      121 ttaacaaaaa aagaacaatt ggttataagg agagagtatg cgtgtcatca ccctggcggg
      181 tagtcctcgc tttccttctc gctccagctc cttgctggaa tatgcgcggg aaaaactaaa
      241 ttgcctggat gtagaggttt atcactggaa tctgcaaaac ttcgccccgg aagatctgct
      301 ttacgctcgt ttcgatagtc cggcactcaa gaccttcacc gaacagctgc aacaggctga
      361 tggactgata gtcgctacac ctgtgtataa agccgcctat tccggcgcgc tgaaaaccct
      421 gctcgacctg ctgccagagc gcgctttgca aggcaaagtg gtgctaccgc tggcgacggg
      481 cggtacggtg gctcatctgc tggcggtcga ttatgccctt aagccagttt taagtgcgat
      541 gaaagcccag gagatcctgc acggcgtgtt tgccgatgac tcacaagtta ttgattacca
      601 tcacaaaccc cagttcacgc caaatctgca aacccgtctt gataccgcgc tagaaacttt
      661 ctggcaggca ttgcaccgcc gcgatgttca ggttcctgac cttctgtctc tgcgaggtaa
      721 tgtccatgcg taacatcatt aaactggcgc tggcgggatt gcttagcgtc tctacttttg
      781 cggttgctgc agaatcctcg cctgaagcgt tacgtatagg ctatcagaaa ggcagtattg
      841 gtatggtatt ggcaaaaagc caccagttac tggaaaaacg ctatccgcaa tcaaaaatct
      901 cctgggtgga gttccccgcg ggtccgcaaa tgttggaagc gttaaacgtt ggcagtattg
      961 atctcggcag taccggggat attccgccaa tctttgccca ggctgccggg gctgatttgg
     1021 tatatgtggg cgtcgagcca ccgaagccca aagccgaagt gattctggtg gcagaaaaca
     1081 gcccgatcaa aaccgtagcc gatcttaaag gtcacaaagt tgcctttcag aaaggttcca
     1141 gttcacacaa ccttttactg cgtgcactgc gtcaggccgg acttaagttt accgatatcc
     1201 aacccactta cctgacgccc gctgatgccc gcgccgcgtt ccagcaaggt aacgttgacg
     1261 cctgggctat ctgggatccc tactactctg ctgcattatt acagggcgcc gtgcgggtgc
     1321 tgaaagacgg caccgatctc aatcaaactg gatcgtttta tctggcagct cgtccctatg
     1381 cagaaaaaaa cggcgctttt attcagggcg tactcgcaac ctttagtgag gccgatgcgt
     1441 taacccgcag ccagcgcgag caaagcatcg ctttactggc aaaaacgatg ggcttaccgg
     1501 caccggtgat tgcctcttac ttagatcatc gccctcctac caccatcaaa ccggttaacg
     1561 ccaaggttgc cgccttacag cagcaaacgg cagatctgtt ttatgaaaat cgtctggtgc
     1621 cgaaaaaagt cgatattcgc cagcgcatct ggcagcccac tcaactggaa ggaaaacaat
     1681 tatgagtctg aatatgttct ggtttttacc gacccatggt gacgggcatt atcttggaac
     1741 ggaagaaggt tcacgcccgg ttgatcacgg ctatctgcaa caaattgcgc aagcggcgga
     1801 tcgtctgggc tataccggtg tgctgatccc aacggggcga tcgtgtgaag atgcgtggct
     1861 ggtggcggcg tcgatgatcc cggtgacgca gcggctgaag tttcttgtcg ccctgcgccc
     1921 cagcgtaacc tcacctaccg ttgccgctcg ccaggccgcc acgcttgacc gtctctcaaa
     1981 tggtcgtgcg ttgtttaatc tggtcacagg cagcgatcca caagagctgg caggcgacgg
     2041 cgtgttcctt gatcatagcg agcgctacga agcctcggcg gaatttaccc aggtctggcg
     2101 gcgcttattg ctgggggaaa ccgtcaattt caacggtaaa catattcatg tgcgcggagc
     2161 aaaactgctc ttcccgccga ttcaacagcc ttatcctccg ctttactttg gcggatcgtc
     2221 agacgttgcc caggagctgg cggcagaaca agttgatctc tacctcacct ggggcgaacc
     2281 gccagaacta gtaaaagaga agatcgaaca agtgcgggcg aaagctgccg cgcatggacg
     2341 caaaattcgt ttcggtattc gtctgcatgt gattgttcgt gaaactaacg acgaagcgtg
     2401 gcaggccgcc gagcggttaa tctcgcatct tgatgatgaa actatcgcca aagcacaggc
     2461 cgcattcgcc cggacggatt ccgtaggaca acagcgaatg gcggcgttac ataacggcaa
     2521 gcgcgacaat ctggagatca gccccaattt atgggcgggc gttggcttag tgcgcggcgg
     2581 tgccggaacg gcgctggtgg gcgatggtcc tacggtcgct gcgcgaatca acgaatacgc
     2641 cgcgcttggc atcgacagct ttgtgctttc gggctatccg catctggaag aagcgtatcg
     2701 ggtcggcgag ttgctgttcc cacatctgga tgtcgccatt ccggaaattc cccagccgca
     2761 gccgctgaat ccgcaaggcg aagcggtggc gaatgatttt atcccccgta aagtcgcgca
     2821 aagctaagga agaaataatg gcaacgccag tgaagaagtg gttattgcgc gttgccccct
     2881 ggtttttacc ggtgggcatc gtggcggtgt ggcaactggc ctcctcagtt ggctggctgt
     2941 cgacgcgtat tttgccctca ccggaagggg tggtgacggc gttctggacg ctctccgcca
     3001 gcggcgaact gtggcagcat ctggcgatca gttcctggcg ggcgctgatt ggcttttcaa
     3061 ttggcggatc gctggggttg attttgggac tgattagcgg gctatctcgc tggggagaac
     3121 ggctgctgga tacctcaatt cagatgttgc gcaacgtacc gcatctggcg ctgatcccgc
     3181 tggtgatttt gtggtttggc attgatgaat ccgcaaaaat ctttctggtg gcgctcggca
     3241 cgctattccc catttatatc aacacctggc atggcatccg taatatcgat cgcggtctgg
     3301 tggaaatggc gcgtagctat ggattatccg gcataccgct gtttatccat gtgatcctgc
     3361 ctggtgccct gccctcaatt atggtcggcg tgcgttttgc gttgggcctg atgtggctga
     3421 cgctgattgt tgccgaaacc atttctgcca attcaggcat tggttatctg gcgatgaatg
     3481 cgcgggagtt tttgcaaacg gacgtggtgg tagtcgctat tattctttac gccctgctcg
     3541 gcaaacttgc tgacgtcagc gcgcagctgt tagagcgcct ctggttgcgc tggaacccgg
     3601 cttatcattt gaaggaggcc acggtatgaa tactgctcgt ctgaaccagg gcacaccatt
     3661 gttgctcaat gcagtaagca aacattacgc ggaaaatatc gtcctgaacc aactggattt
     3721 acatattccg gcaggtcagt ttgtggcggt ggtgggccgc agcggtggtg gcaaaagtac
     3781 cctgctgcgc ctgctggcag gtctggaaac gccaaccgca ggcgatgtgt tagcgggcac
     3841 cacaccgctg gctgagattc aagaagacac gcgaatgatg tttcaggatg cgcgtctgct
     3901 gccgtggaaa tcggtgattg ataacgttgg gttaggcctt aaaggccagt ggcgcgatgc
     3961 cgcgcgtcgg gcgttggctg cagtaggact ggagaatcgc gccggggaat ggcctgccgc
     4021 actttctggc gggcagaaac agcgtgtggc gctggcaaga gcgttgattc atcgaccggg
     4081 tttattgttg cttgatgaac cgctcggggc gctggacgcc ttaacgcgac tcgagatgca
     4141 ggatttgatt gtgtcacttt ggcagcagca cggctttacc gtactgttgg tgacgcatga
     4201 tgtgagcgaa gcggtagcaa tggctgaccg ggtgttgtta attgaagagg gaaaaattgg
     4261 tctggatttg acagtggata tcccccgtcc gcgtcgatta gggtcggtgc gattagcgga
     4321 gctggaagcg gaagtattgc aacgggtaat gcggcgtggg cattctgaac aactgattcg
     4381 gcgtcatggt taatatgccg gatgtggcgc acaaaaccag cattttcaac ggactagtag
     4441 gcctgataag acacgtcaag catcgcatca ggcagttggg cacggctgcc ggatgcggcg
     4501 tgaacgcctt atccagccta caaaaccagc aatttcaacg gagttgtagg cctgataaga
     4561 cgcgtcaagc gtcgcatcag gcattttggc tcaaaagtat caagccagtg ctttggtgat
     4621 cttctcgtac agatcgccag agagattttc cagccctttc aactgttcca gcgccgcgcg
     4681 cattttctcc tgacgtttgg catcataacg tttcaggcga atcagcggtt caatcagacg
     4741 tgaagctacc tgcgggttac ggctgttgag gtcggtaagc atttccacca ggaactggta
     4801 accgctgcca tcttcggcat ggaacgctgc cgggttgctg cccgcaaacg caccaatcaa
     4861 cgaacgaata cggttcgggt tgctcatggt aaatgagcga tgctgcaaca gaccacgcac
     4921 cgtctccagc acattcgccg ccgggctggt ggcttgcagg ataaaccatt tatccatcac
     4981 cagaccgtcc tgatgccact tatcgtcgta ctcctgcatc agcgcgtcac ggcaaggcag
     5041 ctgtgcagca accgccgcag aaagcgctgc cagcgcatcg gtcatattgt ttgcttcatg
     5101 gtattgcttg ctcaccagca cgtcagccag atgcgtttca ccaaaagcaa ggaagcgcag
     5161 gcaggcatta cgcagagtgc gttttgcaat atcttcatgc tcaacacggt actcgctctg
     5221 gtaattcgcg ttgtaaatag ccagcagctc atccgccagt tcagtcgcca gagtacgagt
     5281 gagtgcttcg cgtacttcgg caatagcaat cgggtcgatg atatcgaaca gttcagccat
     5341 ttcattgaca gaaggcagcg tcaggatttc tgccgccagc gccggatcaa tcttctcatc
     5401 gagcagcacc gcgcggaaag cgtcagccac atgtaccggc agagacagcg gctgcccttg
     5461 ctgatgacgg gcgacgttca gcttgatgta ggttgccagc aaactttgcg ccgcatccca
     5521 acgggagaaa tcattacgcg catgacgcat caggaaggtc agttgctgat cgctccactt
     5581 atattccagt ttcactggcg cagagaattc gcacagcagc gcaggcaccg gctggaagta
     5641 gacattatca aaaacaaagg tctgttccgc ctgggtgacg ttcagcacgg aattcaccgg
     5701 atgaccgcct ttctgcaacg ggatcacttt gccttcgttg tcatacaatt cgatggcaaa
     5761 tggaatatgc agcggctgtt tttctgcctg atccggcgtg gctggcgtgc gctggctgat
     5821 ggtcagggtg tactgctcgg tttccggatt gtagtcgtct ttgacggtca caatcggcgt
     5881 gccggactgg ctgtaccaac ggcggaaatg ggagagatcg acattcgacg catcttccat
     5941 cgcctgcaca aagtcgtcgc aggttgccgc gctgccatca tggcgctcga aatagagttg
     6001 catccctttc tggaagtttt cttcgcccag cagggtgtgg atcatgcgaa tcacttccgc
     6061 gcccttctcg taaacggtca gggtgtagaa gttgttcatc tcaatgacca tatccgggcg
     6121 gatcgggtgc gccatcgggc tggcgtcttc cgcaaactgc aatccgcgca tggtgcgcac
     6181 attattgata cggtttaccg cgcgggaacc aaggtcagag ctgaattcct gatcgcggaa
     6241 gacggttaaa ccttctttca ggctgagctg gaaccagtcg cggcaggtca ctcggttacc
     6301 ggtccagtta tggaaatatt catggccgat aacgcgttca atatcgagat aatctttgtc
     6361 ggtggcggtg tcggtgcggg ccaacacata tttggagtta aagatattca gacccttatt
     6421 ctccattgcg cccatattga agaaatccac cgcgacgatc atatagatgt cgaggtcata
     6481 ctccaggcca aagcgttctt catcccattt catggagttt ttcagcgagg tcatcgccca
     6541 cggcgcgcga tcaaggttgc cgcgatcgac gtacagctcc agtgctactt cgcgaccaga
     6601 acgcgtggta aaggtgtcgc gcagtacatc aaagtcgcct gccaccagcg caaacaggta
     6661 gcacggtttc gggaacgggt cctgccactg tacccaatgg cgtccgtttt ccagttcacc
     6721 ttgcgcaacg cggttaccgt tggaaagcag gaagggatat ttggttttat cggcaataat
     6781 tttggtggta aaacgcgcca gcacgtccgg gcggtcgaga taatacgtaa tatggcggaa
     6841 accttcggct tcacactggg tgcaaagcgc atcgcctgac tgataaagcc cttccagcgc
     6901 cgtattcgcc gccgggctta tttcattaat gatcttaagc gtaaaacgct ccggcaaatt
     6961 actgatgacc agtgcgccct cttcttcttt ccaggcggtc cacggctcat cattaatatg
     7021 aacagaaacc agtttgaggt cttcgccgtt gagacgaagc ggagcatctg atgcaccatg
     7081 acggacagcc tggctgaccg cggtaacgac cgtcttttgc gcgtcgaggt caaaggtcaa
     7141 gtcaatatca gtaatctggt aatccggcgc acgataatcg tgacggtatt tggcttgtgg
     7201 ctgttgagtc ataaataacc tttagcatct tttatagagt ctggtgttca gtctattcct
     7261 gttgcgtaaa tcgcgctatg cagaatcttc atcttttcag gtacaaacgc ctttattgct
     7321 acatttttat aacatacacc gcgtaatgcc atcgaccaga aaggtggcat atggtgtgat
     7381 cggggttcaa taaattgcga aacaaggtat actccagcag ttcctgaaga tgtttattgt
     7441 actaaacgct cctgtacgag gacgctactg cgcacctatg acacaattcg cttctcctgt
     7501 tctgcactcg ttgctggata cagatgctta taagttgcat atgcagcaag ccgtgtttca
     7561 tcactattac gatgtgcatg tcgcggcgga gtttcgttgc cgaggtgacg atctgctggg
     7621 tatttatgcc gatgctattc gtgaacagat tcaggcgatg cagcacctgc gcctgcagga
     7681 tgatgaatat cagtggctct ctgccctgcc tttctttaag gccgactatc ttaactggtt
     7741 acgcgagttc cgctttaacc cggaacaagt caccgtatcc aacgataatg gcaagctgga
     7801 tattcgttta agcggcccgt ggcgtgaagt catcctctgg gaagttcctt tgctggcggt
     7861 tatcagtgaa atggtacatc gctatcgctc accgcaggcc gacgttgcgc aagccctcga
     7921 cacgctggaa agcaaattag tcgacttctc ggcgttaacc gccggccttg atatgtcgcg
     7981 cttccatctt atggattttg gcacccgtcg ccgtttttct cgcgaagtac aagaaaccat
     8041 cgttaagcgt ctgcaacagg aatcctggtt cgtgggcacc agcaactacg atctggcgcg
     8101 tcggctttcc ctcacgccga tgggaacaca ggcacacgaa tggttccagg cacatcagca
     8161 aatcagcccg gatctagcca acagccagcg agctgcactt gctgcctggc tggaagaata
     8221 tcccgaccaa cttggcattg cattaaccga ctgcatcact atggatgctt tcctgcgtga
     8281 tttcggtgtc gagttcgcca gtcgctatca gggcctgcgt catgactctg gcgacccggt
     8341 tgaatggggt gaaaaagcca ttgcacatta tgaaaagctg ggaattgatc cacagagtaa
     8401 aactctggtt ttctctgaca atctggattt acgcaaagcg gttgagctat accgccactt
     8461 ctcttcccgc gtgcaattaa gttttggtat tgggactcgc ctgacctgcg atatccccca
     8521 ggtaaaaccc ctgaatattg tgattaagtt ggttgagtgt aacggtaaac cggtggcgaa
     8581 actttctgac agccctggca aaactatctg ccacgataaa gcgtttgttc gggcgctgcg
     8641 caaagcgttc gaccttccgc atattaaaaa agccagttaa tatcatcagg gagctaatcg
     8701 gctccctttt tttaccttta attccgaaat ctttcgctgc atttgcgaat tctgcttgtc
     8761 tgattgcaga tgccaggtaa cataggtatc cccccattta cgggtggaca agtgtttatt
     8821 ttttccgact attaacagag agaatattat gagcgttgtg cctgtagccg acgtactcca
     8881 gggccgcgta gccgttgaca gcgaagtcac cgtacgcgga tgggtacgta cccgccgaga
     8941 ttcaaaagct ggcatctcct tcctcgccgt ttatgacggt tcctgctttg atcctgtaca
     9001 ggctgtcatc aataattctc tgcccaatta caatgaagac gtcctgcgtc tgaccaccgg
     9061 ctgctcggtc attgtgacgg gtaaagtcgt ggcgtcgccg ggccaggggc aacaatttga
     9121 aattcaggcc agcaaggttg aagttgctgg ttgggttgaa gatccagaca cttacccgat
     9181 ggcggcaaaa cgccacagca ttgagtatct gcgtgaagtc gctcacctgc gtccgcgcac
     9241 aaacctgatt ggtgccgtcg cgcgcgttcg tcatacgctg gcgcaggcgc tgcatcgctt
     9301 ctttaacgag cagggattct tctgggtttc aacgccactg attaccgcat ctgataccga
     9361 aggtgcaggc gaaatgttcc gcgtttctac gctggatctg gaaaacctgc cgcgtaacga
     9421 tcagggcaaa gtggatttcg acaaagactt ctttggtaaa gagtctttcc tgaccgtatc
     9481 tggccagttg aacggcgaaa cctacgcttg cgcattatcc aaaatttata ccttcggccc
     9541 gactttccgt gctgaaaact ccaacaccag ccgtcacctg gcggaattct ggatgctgga
     9601 gccggaagtg gcgtttgcta acctgaacga tatcgcgggt ctggctgaag ccatgctgaa
     9661 atatgtcttc aaagcggttc tcgaagaacg cgcagacgac atgaaattct tcgctgaacg
     9721 cgtagataaa gatgccgttt cacgtctgga acgcttcatt gaggccgatt tcgcgcaggt
     9781 ggattacacc gacgcagtga ccattctcga aaactgcggc aggaagtttg aaaacccggt
     9841 ttactggggc gtcgatctct cttctgagca tgagcgttat ctggcggaag aacactttaa
     9901 agcaccggta gtggttaaaa actatccgaa agatattaaa gcgttctata tgcgcctcaa
     9961 cgaagacggt aaaaccgttg cggctatgga cgttctggct ccgggcatcg gtgagatcat
    10021 tggtggctcc cagcgtgaag aacgtctgga cgttctggac gagcgtatgc tggaaatggg
    10081 cctgaataaa gaagattact ggtggtatcg cgatctgcgt cgctacggta ctgttccgca
    10141 ttcaggtttc ggtcttggtt ttgaacgcct gattgcttac gtaactggcg tgcaaaacgt
    10201 gcgtgatgtg attccgttcc cacgtactcc gcgcaacgcc agcttctgat tttcttcctc
    10261 aaagagccag cgcctgctgg ctctttttta tcctttctgt caagttatct gtttgttaag
    10321 tcaagcaatc tatttgcaac cccgccataa cgatcatcct gttacggaat attacattgc
    10381 aacatttacg tacaaaaaat aacccgcatt cttattgcgg attagttttt tcttagctga
    10441 tagcacaatt ttcatactat
//
LOCUS       DAZFWL020000153        10282 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFWL020000153 DAZFWL020000000
VERSION     DAZFWL020000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.153"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1185
                     /gene="ybaT"
                     /locus_tag="ACHKNP_RS20950"
                     /old_locus_tag="pgaptmp_004190"
     CDS             <1..1185
                     /gene="ybaT"
                     /locus_tag="ACHKNP_RS20950"
                     /old_locus_tag="pgaptmp_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU4706255.1"
                     /translation="QAALLMEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDF
                     FRRGLGNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGI
                     IAVMTLFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFF
                     SCIGITFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSA
                     LELEKYADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSERE
                     LPKLMNKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIRL
                     RHDIHASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNK
                     TV"
     gene            1216..1313
                     /locus_tag="ACHKNP_RS20955"
                     /old_locus_tag="pgaptmp_004191"
                     /pseudo
     CDS             1216..1313
                     /locus_tag="ACHKNP_RS20955"
                     /old_locus_tag="pgaptmp_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1310..1717
                     /gene="cueR"
                     /locus_tag="ACHKNP_RS20960"
                     /old_locus_tag="pgaptmp_004192"
     CDS             1310..1717
                     /gene="cueR"
                     /locus_tag="ACHKNP_RS20960"
                     /old_locus_tag="pgaptmp_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HGU4706256.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(1718..2176)
                     /gene="ybbJ"
                     /locus_tag="ACHKNP_RS20965"
                     /old_locus_tag="pgaptmp_004193"
     CDS             complement(1718..2176)
                     /gene="ybbJ"
                     /locus_tag="ACHKNP_RS20965"
                     /old_locus_tag="pgaptmp_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HGU4706257.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(2173..3090)
                     /gene="qmcA"
                     /locus_tag="ACHKNP_RS20970"
                     /old_locus_tag="pgaptmp_004194"
     CDS             complement(2173..3090)
                     /gene="qmcA"
                     /locus_tag="ACHKNP_RS20970"
                     /old_locus_tag="pgaptmp_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGU4706258.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            3236..3913
                     /gene="fetA"
                     /locus_tag="ACHKNP_RS20975"
                     /old_locus_tag="pgaptmp_004195"
     CDS             3236..3913
                     /gene="fetA"
                     /locus_tag="ACHKNP_RS20975"
                     /old_locus_tag="pgaptmp_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HGU4706259.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTNGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            3900..4679
                     /gene="fetB"
                     /locus_tag="ACHKNP_RS20980"
                     /old_locus_tag="pgaptmp_004196"
     CDS             3900..4679
                     /gene="fetB"
                     /locus_tag="ACHKNP_RS20980"
                     /old_locus_tag="pgaptmp_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HGU4706260.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(4742..5596)
                     /gene="cnoX"
                     /locus_tag="ACHKNP_RS20985"
                     /old_locus_tag="pgaptmp_004197"
     CDS             complement(4742..5596)
                     /gene="cnoX"
                     /locus_tag="ACHKNP_RS20985"
                     /old_locus_tag="pgaptmp_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HGU4706261.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAETVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(5657..6466)
                     /gene="ybbO"
                     /locus_tag="ACHKNP_RS20990"
                     /old_locus_tag="pgaptmp_004198"
     CDS             complement(5657..6466)
                     /gene="ybbO"
                     /locus_tag="ACHKNP_RS20990"
                     /old_locus_tag="pgaptmp_004198"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU4706262.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(6456..7079)
                     /gene="tesA"
                     /locus_tag="ACHKNP_RS20995"
                     /old_locus_tag="pgaptmp_004199"
     CDS             complement(6456..7079)
                     /gene="tesA"
                     /locus_tag="ACHKNP_RS20995"
                     /old_locus_tag="pgaptmp_004199"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HGU4706263.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            7050..7736
                     /gene="ybbA"
                     /locus_tag="ACHKNP_RS21000"
                     /old_locus_tag="pgaptmp_004200"
     CDS             7050..7736
                     /gene="ybbA"
                     /locus_tag="ACHKNP_RS21000"
                     /old_locus_tag="pgaptmp_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HGU4706264.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            7733..10147
                     /gene="ybbP"
                     /locus_tag="ACHKNP_RS21005"
                     /old_locus_tag="pgaptmp_004201"
     CDS             7733..10147
                     /gene="ybbP"
                     /locus_tag="ACHKNP_RS21005"
                     /old_locus_tag="pgaptmp_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HGU4706265.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2256 a         2642 c         2868 g         2516 t
ORIGIN      
        1 caggctgcat tgctaatgga agcctcgacc tgggtcgcct ttgcttttgg cggtattgtg
       61 gcgatgtttt ccggttatgc ctatgcgcgt ctgggggcga gctatcccag caatggcggc
      121 attatcgact tctttcgtcg cggattaggc aacggcgtct tttcgctggc gctctcgtta
      181 ctgtacctgt tgacgctggc ggtgagcatc gccatggtcg cccgtgcttt tggcgcttat
      241 gccgtgcagt ttttgcatga aggcagccag gaggagcacc ttattttgct ctacgcgttg
      301 gggatcattg cggtgatgac gcttttcaac tccttaagca accatgcggt agggcggctg
      361 gaagtgatcc tcgtcggcat taaaatgatg atcctgttat tgctgattat tgccggtgtc
      421 tggtcgctgc aaccggcgca tatttccgtc tctgcgcccc ccagctccgg tgcgttcttc
      481 tcctgtatcg ggataacctt ccttgcctat gcgggctttg gcatgatggc gaacgcggcg
      541 gataaagtga aagatccgca ggtcattatg ccgcgggcgt ttctggtggc gattggcgtt
      601 accacgttgc tttatatctc gctggcactg gttttgctta gcgatgtatc ggcattagag
      661 ttagaaaaat atgccgatac cgccgtagcg caggctgctt ctccgctgct cggacatgtg
      721 ggttatgtga tcgtcgtcat cggcgcttta ctggcgacgg cttcagccat taacgcgaac
      781 ctgttcgccg tgtttaacat catggacaac atgggcagcg aacgcgaact gccgaagcta
      841 atgaataaat ccctgtggcg gcagagtacc tggggcaaca tcattgtcgt ggtgttgatt
      901 atgctgatga cggcggcact aaatttaggc tcactcgcca gcgttgccag cgccaccttt
      961 ttgatttgct atctggcggt gtttgtggtg gcgatccgcc tgcgtcatga tattcacgcc
     1021 tcgttgccga ttcttatcgt tggtacgttg gtgatgttgt tggtgatcgt tggctttatc
     1081 tacagtctgt ggtcccaggg tagccgtgcg ttgatatgga ttattggctc actcttactc
     1141 agccttattg tggcaatggt catgaagcgc aataaaaccg tataacatct ctctgtgcgc
     1201 agtacttcct gtattattgt ggtggcggtc gatattcgca ctggcaaaaa aacgtgcttg
     1261 aatatctgtt gaaacccttt aacaaagcac aggaggcgtt gcgcgaacga tgaacatcag
     1321 cgatgtagca aaaattaccg gcctgaccag caaagccatt cgcttctatg aagagaaggg
     1381 gctggtgacg ccgccgatgc gcagcgaaaa cggttatcgc acctacacgc agcagcatct
     1441 caacgaactg accttactgc gccaggcacg gcaggtgggc tttaacctgg aagagagcgg
     1501 cgagctggtg aatctgttta acgacccgca gcggcacagc gccgacgtca aacggcgcac
     1561 gctggagaag gtggcggaga tcgaacgaca cattgaggag ctgcaatcca tgcgcgacca
     1621 gctgctggca ctggcgaatg cctgccctgg cgatgacagc gccgactgcc cgattatcga
     1681 aaatctctcc ggctgctgtc atcatcgggc agggtgatta agacgagacg gctcggatat
     1741 gtagggttat cccttctatc gcaatgactt caacatgcgt acctgcaccg agatcctcgc
     1801 tggcgctgac aggccatgaa ctgtcaccga cgcgcatatg accgcgcccg ttgaccagcg
     1861 gagattccag cacaaaacgt cggccaatca gctgctgccc gcgctggttt aaatgactat
     1921 cgctgtgctt ttgttcgcgc acccgccgcg acaaccattt ccaccacagc caggcggcga
     1981 gcagcgtcag gatggcaaac attacccctt gccactccca acccagcggc accagccaga
     2041 ccaccaggcc ggtaatcacc gctgccacgc cgctccacaa caaataacca tttccgccca
     2101 gcatctcggc tgccagcagc aaaccgccga gactgagcca gaaaatatgt ggatgaacga
     2161 ccattaactc catcatggct gagtccgttt gttggcgctg tctttcacca gctcggcaat
     2221 cccggcaatc gaccccatca ggctgctggc ctctaatggc atcatcacta ctttgctgtt
     2281 actggaggaa ccgatctgct gtaacgcttc ggtgtatttc tgcgctacga agtagttcac
     2341 cgcctgaata tcaccggagg cgatagcttc agacaccatt ttggtggcgc gggcttctgc
     2401 ttcggcggaa cgttcacgcg cttcagcctg taaaaacgcc gactgacgtt cgccttccgc
     2461 tttcaggatt tgcgactgtt tttcaccttc ggctttgagg atttccgcct gacggatccc
     2521 ttccgcttca agaatgtaag cgcgtttggt acgttccgct ttcatctgcg cgttcattga
     2581 agagataagc tctgccggtg ggcgcacgtc gcgaatttca atacgggtga ctttaatccc
     2641 ccacgggttg gtggcttcat cgacaatacg cagcaggcgt gagttgatgc tgtcgcgctg
     2701 agagagcatt tcgtcaagtt ccattgaacc caacacggta cggatgttag tcatggtcag
     2761 gttgatgatc gccagctcca gattgctgac ttcataagcc gcgcgcggcg cgtcaatcac
     2821 ctgaataaaa cagactgcgt cgatggtaac gttggcgtta tctttcgaga taacttcctg
     2881 ggaagggata tcgagcactt gctccatcat attgatcttg cgaccaatgc gatccataaa
     2941 cggcaccacc agactgagcc ccggctgtaa cgttttggta tagcgaccaa aacgttctac
     3001 cgtccattga tagccctgcg gcacgatttt gacgcccgcg ccgacaatga ccagcgcgac
     3061 aaaaatgaga atcgggataa agataagcat cggaaaaacc tcctgttgta ccgtccataa
     3121 tcagcaaaat tgctgcttga ttaaacaaat tatacctgat tactgaaaga gagttccccc
     3181 ttattcctgc gaaggataaa ctgtttttag taaaaatcag aaaaagggaa cagtgatgca
     3241 ggaaaatagt cctttacttc agctacaaaa cgtaggatat ctggcgggtg atgcgaagat
     3301 tcttaataac atcaattttt cgctgcgtgc tggcgaattt aagttaatta ccggtccttc
     3361 cggttgtggc aaaagtacgc tgctaaaaat agttgcttca ttgatcagcc caaccaacgg
     3421 aacgttactg tttgaaggtg aggatgtcag cacactaaag ccagaaatct accgccagca
     3481 agtctcttac tgcgcccaga cgccgacgct gtttggcgat acggtatacg ataatctgat
     3541 ctttccctgg cagatccgta accagcagcc tgacccggcc atttttctcg attttctcga
     3601 acgcttcgct ttgccggaca gcattttgac gaaaaatatc gccgagctat ctggtggtga
     3661 aaaacaacgc atctcactga ttcgtaacct gcaatttatg ccgaaggttt tattgctgga
     3721 tgaaatcacc agtgcgctgg atgaaagtaa taaacataac gttaatgaga tgatccatcg
     3781 ttatgtgcgc gagcaaaata ttgccgtgct gtgggtgaca cacgataaag acgaaattaa
     3841 tcatgcggat aaagtgatta cactgcaacc gcatgccgga gaaatgcagg aagcacgcta
     3901 tgaactcgca taatattact aacgaatcat tggcactggc attaatgctg gtggtggtgg
     3961 caatcttaat tagccataaa gaaaaactgg cgctggagaa agatattttc tggagcgtcg
     4021 ggcgagcgat aattcagctg attattgtcg gctatgtgct gaagtatatt ttcagcgtgg
     4081 atgatgccag cctgacatta ttgatggtgt tatttatctg ctttaatgcg gcgtggaacg
     4141 cgcaaaaacg cagtaaatat attgctaaag cttttatctc atcgtttatt gccattacgg
     4201 tcggggcggg aattaccctg gcggtgctga ttctctctgg gtcgattgaa tttatcccga
     4261 tgcaggtgat ccctatcgcc gggatgattg ccggtaacgc catggtagcg gtggggttgt
     4321 gttacaacaa tttagggcaa cgggtcatta gcgaacagca acagattcag gagaaactga
     4381 gtcttggtgc gacgccgaag caggcttcag cgatattgat tcgcgacagt attcgcgcgg
     4441 ctttaattcc gacggtcgat tcagcaaaaa cggttggctt agtgagttta cctggaatga
     4501 tgtccgggct gatatttgcc gggattgatc cggtgaaggc gattaaatat cagattatgg
     4561 tgacctttat gctgctctca accgccagct tgtcgaccat tattgcctgc tatttaacct
     4621 atcgtaagtt ttataattcg cgccaccagt tggtggtgac gcaattgaag aagaaatgat
     4681 gcgatgccgg atgcgcagca tcgcatccgg cgttgtggtt catgtgccga tcaacggaat
     4741 atcaatacaa caatgcatac agctggcggc gatacttcga cgccagtgca tcacccgtac
     4801 ccagcgcagc gaggatctcc tgaaacgttt tacgcgtctg accgtctgcg gcggtgagat
     4861 ctttacgcag atgcccgaac agcaactcca gcgcctcttc attgcgccca acctggtgca
     4921 gttgcagagc cagttgcgtc gccagtgcgg catcttctgg attctccgcc acctgctgtt
     4981 gcaactgttg aatttccggc gtatcagccg cctgcttcag cagttcgatt tgcgccacca
     5041 gcccctggta gcgggtgtcc tgatcctgca acggaatggt tttcagcacc gtttccgcat
     5101 cttcagaacg gttcagcgca atcagcgttt ctgccagcag caggccgatc tccccgttct
     5161 gattcgacaa ctgccaggcg tctttcagca atggcagggc atcggtgtaa ttgccttcct
     5221 gcatcagttg catcgcctgc tgcgctttca gctcttcttc gcgcggcagc actttatcca
     5281 gcagggcgcg gatcgcctct tccggttgcg gcccctggaa gccatctacc ggttgcccgt
     5341 tctggaacag atacacggtc ggaatcgcgc gcagaccaaa ctgggcggca atcatctgct
     5401 ccgcgtcgca gtccagcttc gccagaataa attgcccgtt gtactgcgcc gcgaggcttt
     5461 ccaggatcgg ggttaactgc aaacagtgct ggctacgttc agaccaaaaa tagaacagca
     5521 ccggagtggt catcgactgt tcaagaacct gttgcaggtt agattcgtta atgttgacaa
     5581 tattttctac ggacatggag tcgctctctg ttgtcgatat tttctttgac atgggggctt
     5641 aagcgcgcgc ttcaactcac ccctgcaata ttttgtccat cacgcgcccc ggcagcaggc
     5701 gcttgagcac cattactgcc caggtcacca gcgttaccgg atagcgcatc ttcggcttct
     5761 cgctaataaa agcatggcgt actttgtcca ccaccgcttc cggtcccaac gtaaagcggg
     5821 cggcgatgcc gggattttcg actggtttat cactttgcgt ctggttgacg ttgtcggtga
     5881 agcgagtacg aatgggaccg ggttcgatca ggctgacttt aattccgctg tggcgcagct
     5941 ccatgcgcag tgcgtctgac cacgcctcca gcgcatattt actggccgcg taagcgccac
     6001 gacccggcgt ggagattaat cccatcaccg atgatgtcat cacaatacgc ccttcaccgt
     6061 gcggtaacat cgcgggtaac aggcgcatgg tgagctggtg tgcgccgaaa aagttggcgg
     6121 aaaactgctg ttccatctgc gcacggctga tggtggaaag ggggccatac atgccgaatc
     6181 cggcattgtt aaagatccca tacagacaat tatcggtcag ggcgatcacc tcgtctgctg
     6241 cgcgatcaac actttctggt gaatccagat cgatcaacac gccggtaaat cccatgttgt
     6301 tcatacgctc aacatcatcc ggttttcggc aacctgccag cacatgaaaa ccctggcgtt
     6361 ttaattcgag cgcgctttcc aggccaattc cactggaaca tccggtaatt aagaccgatt
     6421 tttgcataac tttacctgtc aggatctccg ttgctttatg agtcatgatt tactaaaggc
     6481 tgcaactgct tcgccatcca gtcggcaata aacggctggg cgtcgcggtt gggatgaata
     6541 ccgtcatcct gcatccattg tggcttgagg tagacctctt ccataaaaaa gggcagcagc
     6601 ggaacatcaa actctttggc gagtttgggg taaatggcgc taaaggcttc attataacgg
     6661 cgaccatagt ttgcaggcag acgtatttgc attaacaatg gttcagcgtt ggcggctttg
     6721 acatcctgca aaatctggcg cagcgtttgc tcggtttgct gtggctgaaa accacgcaaa
     6781 ccgtcattgc cgcccagttc aaccagcacc cagcgcggct gatgctgttt cagcagagcc
     6841 ggaaggcgcg ccagtccttg ttgcgaggtg tcgccgctga tgctggcatt aactaccgac
     6901 gttttactct gccacttatc attcaacaag gcaggccagg ccgcgctggc agacattcga
     6961 tacccggcgc tcaggctatc acccagaatc aataacgtgt ccgctgcggc ggcacggaag
     7021 gttaacagga ccaggaacag gaagggcaaa tgccagcgga aaacattgtt gaagttcatc
     7081 atcttaagaa gtccgtcggt cagggggagc atgaactctc catcctcacc ggagttgagc
     7141 tggttgtcaa acgtggcgag accatcgcac tggtgggcga atcgggatcg ggtaagtcaa
     7201 ccttgctggc gatcctcgcc gggcttgatg acggcagcag tggcgaagtg agtctggtgg
     7261 gacaaccgct acataatatg gacgaagaag cgcgggcaaa gttgcgcgcg aagcacgtcg
     7321 gctttgtttt tcagtcattt atgttaattc ctacccttaa cgcgctggaa aatgtcgagc
     7381 ttccggctct gctgcgcggt gagagtagcg cggaaagtcg taacggggcg aaagcgttgc
     7441 tcgaacagtt agggctgggt aaacgtctgg atcatcttcc ggcacagctt tccggcggtg
     7501 aacagcaacg agtggcgctg gcacgagcct ttaatggtcg acctgatgtg ctgtttgccg
     7561 acgaacccac cggcaacctt gaccgccaga cgggcgataa aattgccgac ctgctgtttt
     7621 ccctcaaccg tgaacatggc accacgttga ttatggtgac tcacgacctg caactggcgg
     7681 cacgctgcga ccgctgctta cggctggtga acgggcagtt gcaggaggaa gcatgattgc
     7741 acgttggttc tggcgcgaat ggcgctcgcc gtcgctatta attgtctggc tggcgctaag
     7801 cctggcggtg gcctgcgtgc tggcgctggg caatatcagc gatcgcatgg agaagggctt
     7861 aagccagcaa agccgtgagt ttatggcggg cgatcgggcg ttgcgcagtt cgcgcgaagt
     7921 gccgcaagca tggctggagg aagcgcaaaa gcgcggcctg aaagtcggca agcagctgac
     7981 tttcgccacc atgacatttg caggcgacac accgcagctg gcgaacgtca aagcggtgga
     8041 tgatatctac ccgatgtatg gcgatctgca aactaatccc cctggcctga aaccgcaggc
     8101 gggcagcgta ttgctggccc cacgcctgat ggcactgctt aacctgaaaa cgggcgacac
     8161 cattgacgtg ggcgatgcca ccttgcggat tgccggagaa gtgattcagg aaccggattc
     8221 cggttttaac cccttccaga tggctccgcg tctgatgatg aatctggcgg atgtcgataa
     8281 aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt tataaattcg gcggcaacga
     8341 gaaccagctc gacggctatg agaaatggtt gttacctcag cttaaacccg aacaacgctg
     8401 gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg atggaacgct cgcaacagtt
     8461 cctgctgctt tcggcgcttc tgaccttgct gctggcagtg gcagcggtgg cggtagcgat
     8521 gaatcattac tgtcgcagtc gctacgatct ggtggcgatc ctcaaaacgc tgggggcagg
     8581 gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg atggtgctgg cgctttcagc
     8641 cgttaccggt ggggcgatag gcctgttgtt cgaaaacgtg ttgatggtgc tgctcaagcc
     8701 cgttctgcct gctgcactac cgccagccag cctctggccg tggctgtggg cgctgggtac
     8761 catgacggtc atctcgctgc tggtggggct acgaccgtac cgcctgttgc tggcaacgca
     8821 gcctttacgc gtattacgta atgatgtggt agcgaacgtc tggccgctga agttttatct
     8881 gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga ttaatgggtg gcagcatgct
     8941 gctttgggcg gtgctggcgg gcgcggtagt actggctttg ctgtgcggtg tgctgggctg
     9001 gatgctgctg aatgtacttc gccgcatgac gctgaaatcg ctacctctgc gcctggcggt
     9061 tagccgcctg ttacgtcagc cgtggtcaac attaagccag ctttcggcat tttcgctctc
     9121 ctttatgctg ctggcgctgt tgctggtatt gcgtggcgat ctgcttgacc gctggcagca
     9181 gcagctacct ccagaaagcc cgaactactt tttaattaac attgccacag aacaggtaga
     9241 accgctaaaa gcgttcctcg cggaacatca gatagtcccg gaatcatttt atccggtagt
     9301 gcgggcgcgg ctgacggcga ttaacgataa gccgacagaa ggtaatgaag atgaggcgct
     9361 taaccgcgaa ctcaatctta cctggcaaaa tacgcgaccc gatcataatc cgattgtcgc
     9421 cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa gaggggctgg cgaaacgctt
     9481 aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat acccaggagt tccgcgctaa
     9541 agtgaccagc ctgcgcaaag tggactggga aagtctgcgg cctaatttct attttatttt
     9601 ccctgaaggg gcattagacg ggcaaccgca gagctggctt accagtttcc gctgggagaa
     9661 tggcaacggc atgttgacac aactcaaccg ccagttcccg accattagcc tgttagatat
     9721 tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag gtaagtcggg cgctggaggt
     9781 gatggtggtg ctggtcaccg cctgcggtat gttgctgttg ctggcacagg tgcaggtggg
     9841 aatgcgccag cgtcatcagg agctggtggt atggcgcaca ctcggtgcgg ggaaaaaact
     9901 gctgcgcacg acattgtggt gtgagttcgc catgcttggg tttgtttccg gcctggtggc
     9961 cgcaattggt gcggaaacgg cactggcagt gttgcaggcg aaagtgtttg atttcccgtg
    10021 ggagccagac tggcgattgt ggattgttct gccgtgcagc ggtgcgctgc tgctgtcgct
    10081 tttcggcggc tggctgggtg cgcgactggt taagggtaag gcgctgttca ggcagtttgc
    10141 ggggtgatga gagtaaataa ggggcgttct ggttgaatgg aacgccttgt ttaaattaat
    10201 tctacaatat gtttcattga tgatgttatt gaattggtga tttcctatct ttctattgcg
    10261 atattaatct gaatcggtgg ag
//
LOCUS       DAZFWL020000154        10158 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFWL020000154 DAZFWL020000000
VERSION     DAZFWL020000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.154"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            99..1493
                     /gene="galP"
                     /locus_tag="ACHKNP_RS21010"
                     /old_locus_tag="pgaptmp_004202"
     CDS             99..1493
                     /gene="galP"
                     /locus_tag="ACHKNP_RS21010"
                     /old_locus_tag="pgaptmp_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HGU4706266.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1570..2067
                     /locus_tag="ACHKNP_RS21015"
                     /old_locus_tag="pgaptmp_004203"
     CDS             1570..2067
                     /locus_tag="ACHKNP_RS21015"
                     /old_locus_tag="pgaptmp_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HGU4706267.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2162..2869
                     /gene="endA"
                     /locus_tag="ACHKNP_RS21020"
                     /old_locus_tag="pgaptmp_004204"
     CDS             2162..2869
                     /gene="endA"
                     /locus_tag="ACHKNP_RS21020"
                     /old_locus_tag="pgaptmp_004204"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HGU4706268.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2949..3680
                     /gene="rsmE"
                     /locus_tag="ACHKNP_RS21025"
                     /old_locus_tag="pgaptmp_004205"
     CDS             2949..3680
                     /gene="rsmE"
                     /locus_tag="ACHKNP_RS21025"
                     /old_locus_tag="pgaptmp_004205"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGU4706269.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3693..4643
                     /gene="gshB"
                     /locus_tag="ACHKNP_RS21030"
                     /old_locus_tag="pgaptmp_004206"
     CDS             3693..4643
                     /gene="gshB"
                     /locus_tag="ACHKNP_RS21030"
                     /old_locus_tag="pgaptmp_004206"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HGU4706270.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4752..5315
                     /locus_tag="ACHKNP_RS21035"
                     /old_locus_tag="pgaptmp_004207"
     CDS             4752..5315
                     /locus_tag="ACHKNP_RS21035"
                     /old_locus_tag="pgaptmp_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HGU4706271.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5315..5731
                     /gene="ruvX"
                     /locus_tag="ACHKNP_RS21040"
                     /old_locus_tag="pgaptmp_004208"
     CDS             5315..5731
                     /gene="ruvX"
                     /locus_tag="ACHKNP_RS21040"
                     /old_locus_tag="pgaptmp_004208"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGU4706272.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5907..6887)
                     /locus_tag="ACHKNP_RS21045"
                     /old_locus_tag="pgaptmp_004209"
     CDS             complement(5907..6887)
                     /locus_tag="ACHKNP_RS21045"
                     /old_locus_tag="pgaptmp_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HGU4706273.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWSARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLYQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            6905..7609
                     /gene="yggS"
                     /locus_tag="ACHKNP_RS21050"
                     /old_locus_tag="pgaptmp_004210"
     CDS             6905..7609
                     /gene="yggS"
                     /locus_tag="ACHKNP_RS21050"
                     /old_locus_tag="pgaptmp_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HGU4706274.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7627..8193
                     /gene="yggT"
                     /locus_tag="ACHKNP_RS21055"
                     /old_locus_tag="pgaptmp_004211"
     CDS             7627..8193
                     /gene="yggT"
                     /locus_tag="ACHKNP_RS21055"
                     /old_locus_tag="pgaptmp_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HGU4706275.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8190..8480
                     /gene="yggU"
                     /locus_tag="ACHKNP_RS21060"
                     /old_locus_tag="pgaptmp_004212"
     CDS             8190..8480
                     /gene="yggU"
                     /locus_tag="ACHKNP_RS21060"
                     /old_locus_tag="pgaptmp_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HGU4706276.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8488..9081
                     /locus_tag="ACHKNP_RS21065"
                     /old_locus_tag="pgaptmp_004213"
     CDS             8488..9081
                     /locus_tag="ACHKNP_RS21065"
                     /old_locus_tag="pgaptmp_004213"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HGU4706277.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTHEEKSAISHRGQALKLLLDALRNG"
     gene            9074..>10158
                     /gene="hemW"
                     /locus_tag="ACHKNP_RS21070"
                     /old_locus_tag="pgaptmp_004214"
     CDS             9074..>10158
                     /gene="hemW"
                     /locus_tag="ACHKNP_RS21070"
                     /old_locus_tag="pgaptmp_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGU4706278.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKSSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQIT"
BASE COUNT         2373 a         2694 c         2704 g         2387 t
ORIGIN      
        1 gtgtaagcga ttacactgat gtgatttgct tcacatcttt ttacgtcgta ctcacctatc
       61 ttaattcaca ataaaaaata accatattgg agggcatcat gcctgacgct aaaaaacagg
      121 ggcggtcaaa caaggcaatg acttttttcg tctgcttcct tgccgctctg gcgggattac
      181 tctttggcct ggatatcggt gtaattgctg gcgcactgcc gtttattgca gatgaattcc
      241 agattacttc gcacacgcaa gaatgggtcg taagctccat gatgttcggt gcggcagtcg
      301 gtgcggtggg cagcggctgg ctctccttta aactcgggcg caaaaagagc ctgatgatcg
      361 gcgcaatttt gtttgttgcc ggttcgctgt tctctgcggc tgcgccaaac gttgaagtac
      421 tgattctttc ccgcgttctg ctggggctgg cggtgggtgt ggcctcttat accgcaccac
      481 tgtacctgtc agaaattgcg ccggaaaaaa ttcgcggcag tatgatctcg atgtatcagt
      541 tgatgatcac tatcgggatc ctcggtgctt atctttctga taccgccttc agctacaccg
      601 gtgcatggcg ctggatgctg ggtgtgatta tcatcccggc aattttgctg ctgattggtg
      661 tcttcttcct gccagacagc ccacgttggt ttgccgccaa gcgccgtttt gttgatgccg
      721 aacgcgtgct gctacgcctg cgtgacacca gcgcggaagc gaaacgcgaa ctggatgaaa
      781 tccgtgaaag tttgcaggtt aaacagagtg gctgggcgct gtttaaagag aacagcaact
      841 tccgccgcgc ggtgttcctt ggcgtactgt tgcaggtaat gcagcaattc accgggatga
      901 acgtcatcat gtattacgcg ccgaaaatct tcgaactggc gggttatacc aacactaccg
      961 agcaaatgtg ggggaccgtg attgtcggcc tgaccaacgt acttgccacc tttatcgcaa
     1021 tcggccttgt tgaccgctgg ggacgtaaac caacgctaac gctgggcttc ctggtgatgg
     1081 ctgctggcat gggcgtactc ggtacaatga tgcatatcgg tattcactct ccgtcagcgc
     1141 agtatttcgc catcgccatg ctgctgatgt ttattgtcgg ttttgccatg agtgccggtc
     1201 cgctgatttg ggtactgtgc tccgaaattc agccgctgaa aggccgcgat tttggcatca
     1261 cctgctccac cgccaccaac tggattgcca acatgatcgt tggcgcaacg ttcctgacca
     1321 tgctcaacac gctgggtaac gccaacacct tctgggtgta tgcggctctg aacgtactgt
     1381 ttatcctgct gacattgtgg ctggtaccgg aaaccaaaca cgtttcgctg gaacatattg
     1441 aacgtaatct gatgaaaggt cgtaaactgc gcgaaatcgg cgctcacgat taatctcctc
     1501 aggcttcctc ccatcgcgga ggaagccacc tcttgcagtc atcttttctt cgctctatcc
     1561 tctgccgcta tgaaaacatc ccgtctccct atcgccatcc agcaggccgt tatgcgccgc
     1621 ctgcgggaaa aactcgccca ggccaacctg aagctagggc gtaactaccc ggagccaaaa
     1681 ctctcttaca cccagcgcgg aacctccgcc ggaacagcct ggctggaaag ctatgaaatc
     1741 cgcctcaatc ccgttttgct gttggaaaac agtgaagctt ttattgaaga agtggtaccg
     1801 cacgaactgg cacatttgct ggtatggaaa catttcggcc gcgtagcgcc acatggcaaa
     1861 gagtggaagt ggatgatgga aagcgtgctg ggcgttcccg cccgtcgtac gcatcagttc
     1921 gaactgcaat ccgtgcgtcg caacaccttc ccctaccgct gcaagtgcca ggagcatcag
     1981 cttaccgtac gccgccataa tcgcgtagtt cgtggcgagg ccgtctatcg ctgtgttcac
     2041 tgcggtgaac agctggttgc gaaataacca tctgaactat caggaacttt cctgatctgg
     2101 ctgattgcat accaaaacaa ctttcgctac gttgctggct cgttttaaca cggagtaagt
     2161 gatgtaccgt tatttgtcta ttgctgcggt ggtactgagc gcagcatttt ccggcccggc
     2221 gttggccgaa ggtatcaata gtttttctca ggcgaaagcc gcggcggtaa aagtccacgc
     2281 tgacgcgccc ggtacgtttt attgcggatg taaaattaac tggcagggca aaaaaggcgt
     2341 tgttgatctg caatcgtgcg gctatcaggt gcgcaaaaat gaaaaccgag ccagccgcgt
     2401 agagtgggaa cacgtcgttc ccgcctggca gttcggtcac cagcgccagt gctggcagga
     2461 cggtggacgt aaaaactgcg ctaaagatcc ggtctatcgc aagatggaaa gcgatatgca
     2521 taacctgcaa ccgtcagtcg gcgaggtgaa tggcgatcgc ggcaacttta tgtacagcca
     2581 gtggaatggc ggcgaaggcc agtacggtca atgcgccatg aaggtcgatt tcaaagaaaa
     2641 agctgccgaa ccaccagcgc gtgcacgcgg tgccattgca cgcacctact tctatatgcg
     2701 cgaccaatac aacctgacac tctctcgcca gcaaacgcag ctgttcaacg catggaacaa
     2761 gatgtatccg gttaccgact gggagtgcga gcgcgatgaa cgcatcgcga aggtgcaagg
     2821 caatcataat ccgtatgtgc aacgcgcttg ccaggcgcga aagagctaac ctacactagc
     2881 gggactcttt ttgttaaccc ctaccccacg cgtacaaccg cgtggggaga cgacgcggat
     2941 ttttaactat gcgtatcccc cgcatttatc atcctgaacc actgaccagc cattctcaca
     3001 tcgcgctttg cgaagatgcc gccaaccata tcgggcgcgt actgcgcatg gggccggggc
     3061 aggcgttgca attgtttgac ggtagcaacc aggtctttga cgccgaaatt accagcgcca
     3121 gcaaaaaaag cgtggaagtg aaggtgctgg aaggcaagat cgacgatcgc gaatctccgc
     3181 tgcatattca cctcggtcag gtgatgtcgc gtggtgaaaa aatggaattt acgatccaga
     3241 aatcgatcga actcggtgta agcctcatta cgccactttt ttctgagcgc tgcggcgtta
     3301 aactggatag tgaacgtctg aacaagaagc tccagcagtg gcagaagatt gcaattgctg
     3361 cctgtgagca gtgtggtcgt aaccgggtgc cggaaatccg cccagcgatg gatctggaag
     3421 cctggtgtgc agagcaggat gaaggactga aactgaatct tcacccgcgc gccagtaaca
     3481 gcatcaatac gttgccgtta ccggttgaac gcgtccgcct gctgattggc ccggaaggcg
     3541 gtttatcggc agatgaaatt gccatgactg cccgctatca atttactgat atcctgttgg
     3601 gacctcgcgt tttgcgtaca gagacaactg cgctcaccgc cattaccgcg ctacaagtac
     3661 gatttggcga tttgggctaa cggagaagaa taatgatcaa gctcggcatc gtgatggacc
     3721 ccatcgcaaa catcaacatc aagaaagatt ccagtttcgc tatgttgctg gaagcacagc
     3781 gtcgtggtta cgaacttcac tatatggaga tgggcgatct gtatctgatc aatggcgaag
     3841 cccgcgccca tacccgcacg ctgaacgtga agcagaacta cgaagagtgg ttttcgttcg
     3901 tcggtgaaca ggatctgccg ctggccgatc tcgatgtgat cctgatgcgt aaagacccgc
     3961 cgtttgatac cgagtttatc tacgcgacct atattctgga acgtgccgaa gagaaaggga
     4021 cgctgatcgt taacaagccg cagagcctgc gcgactgtaa cgagaaactg tttaccgcct
     4081 ggttctctga cttaacgcca gaaacgctgg ttacgcgcaa taaagcgcag ctaaaagcgt
     4141 tctgggagaa acacagcgac atcattctta agccgctgga cggtatgggc ggcgcgtcga
     4201 ttttccgcgt gaaagaaggc gatccaaacc tcggcgtgat tgccgaaacc ctgactgagc
     4261 atggcactcg ctactgcatg gcgcaaaatt acctgccagc cattaaagat ggcgacaaac
     4321 gcgtgctggt ggtggatggc gagccggtac cgtactgcct ggcgcgtatt ccgcaggggg
     4381 gcgaaacccg tggcaatctg gctgccggtg gtcgcggtga acctcgtccg ctgacggaaa
     4441 gtgactggaa aatcgcccgt cagatcgggc cgacgttgaa agaaaaaggg ctgatttttg
     4501 ttggtctgga tatcatcggc gaccgtctga ctgaaattaa cgtcaccagc ccaacctgta
     4561 ttcgtgagat tgaagcagag tttccggtgt cgatcaccgg aatgttaatg gatgccatcg
     4621 aagcacgttt acagcagcag taacccacct tagcgagaag gatctcgttg agactctgag
     4681 tgacagcgcc cttctttcca cgcatactgg gcgctgttgc ttttttgaac caggaaacag
     4741 aacctctgac aatgaattta cagcatcact ttcttattgc catgcctgct ctccaggatc
     4801 cgattttccg tcgttccgtg gtctacattt gcgaacataa taccaatggt gcaatgggga
     4861 tcatcgtcaa caagccgctg gaaaatctca aaattgaagg gattctggaa aagctgaaga
     4921 tcacgccgga gccgcgtgat gaatcaatcc gtctggataa accggttatg ctcggcggtc
     4981 cgctggctga agatcgcggg tttattttgc atactccgcc ctccaatttt gcttccagca
     5041 ttcgcatttc agacaacaca gtgatgacca cttcccgcga tgtgctggaa acgctcggca
     5101 ccgataaaca accgtctgac gtattggtgg ctctgggtta tgcctcctgg gaaaaaggtc
     5161 aactggaaca agaaattctc gataacgcgt ggctaacggc cccggcggat ctgaatattc
     5221 tgttcaaaac gccgattgcc gaccgctggc gcgaggcggc aaaactgatt ggtgtggata
     5281 ttctcaccat gcctggtgtg gcagggcacg cctgatgagt ggaaccttac tcgccttcga
     5341 cttcggcacc aaaagcattg gcgtagcggt cggccaacgc attaccggca ctgctcgccc
     5401 tttgcctgca attaaagcac aggacggtac gccggactgg aacctcatcg aacgtttact
     5461 gaaagagtgg cagccggacg aaatcatcgt cggtttgccg ctgaatatgg acggcaccga
     5521 gcaaccattg actgccagag cgcgtaaatt tgccaaccgt attcatggtc gtttcggtgt
     5581 tgaagtaaag ctccatgacg aacgtcttag caccgtggaa gcccgttccg gtctgtttga
     5641 acagggcggc tatcgggcgc tcaacaaagg caaagtcgac tctgcctctg cggttattat
     5701 tctcgaaagc tatttcgagc agggatatta aggcgatcta aaagcctggc ggatagtcgt
     5761 taccgccatc ttgccggatg cggcgtgaac gccttatcca gcacacatct ggcagcggct
     5821 gtaggtctga taagacgcgt tagcgtcgca tcagacattt attgccttta taggccctat
     5881 aaacttgcgc atcgggcatg gcaacgtcac aaacgccctt cccccacccg ctgctgataa
     5941 ctctgctgaa acgttaacat ccccacctgc tgcccggttt gaataacatg cggtaactgg
     6001 tgggttttcc cttcgcgaat caaattcccc accgcgggag tgttaatcaa taattcaaac
     6061 agcgccacgc gtccttcctg tttatccact tccagctttt gtgacaacac ggcccgtaaa
     6121 ctacctgcca gttgattacg tacgggatct ttttcctgcg ccggaaatga atccaccagt
     6181 cgctcaactg cctgcgcggc accacgcgta tgtaatgttg ccagcaccaa atgcccggtt
     6241 tctgccgccg tcagtgccag acggattgtc tcgctgtcac gcagctctcc gagcaaaatc
     6301 acatcaggat cttcccgcaa tgcggcccgc aatcccgatg cgaacgtcat acagtgcaaa
     6361 ccaatttccc gctgctggat caaacatcgc tggctggcat agatatattc aacaggatct
     6421 tccagcgtca gaatatgcgc atcggcatgt tgatagagat agccaaccat cgccgctagc
     6481 gtggtagatt tgccgctccc cgtcgccccc gtcaccagaa tcaggccatt ctcgctcttg
     6541 agtaattccg gcaataccgt tggtgcgcca agctgttcga gctgtggaca gtgcgaaggt
     6601 aacaaccgta atgccagaga aataccttgc cgttgcgcga acgcactgcc acgcaaccgc
     6661 tggttttccg ccagcgacac ggcaaaatcc agctggccat tttccagcaa tattgcccgc
     6721 tgatcgtcat ccagccactc ccgcagtagc tcttcgacgt ccggcgcatc aaacggcgca
     6781 gcttccatta gcccgcgaat gcgccatcgt gcggaccagg cgctgcacag gtgtagatcc
     6841 gagacgttat gctttacact aagggccaca atttcttcca tattcatact aagatcctcg
     6901 gaaaatgaac gatattgcgc ataacctggc acaggtccgg gacaaaatct cagcggctgc
     6961 aacgcgttgc ggccgttctc cagaagaaat tacgctgctt gcagtcagta aaacaaaacc
     7021 tgcgagcgcc atcgcagaag ccattgatgc cgggcagcgt caatttggtg aaaactacgt
     7081 tcaggaaggg gtagataaaa ttcgccactt tcaggaactg ggcgtaacag gattagaatg
     7141 gcattttatt ggcccgttgc agtctaataa aagccgcctg gtggcagagc atttcgactg
     7201 gtgtcatacc atcgaccgtt tgcgcatcgc tacccgcctc aacgatcagc gcccggcaga
     7261 acttccccct cttaacgtac tgattcaaat taatattagt gatgaaaaca gtaagtccgg
     7321 gattcaactg gcagaactgg acgagctggc agctgcggtc gctgaactac cgcgtttacg
     7381 tctgcgcggg ctgatggcaa tccctgcacc tgagtcagaa tatgtaaggc agtttgaagt
     7441 tgcacgccaa atggctgtag catttgccgg actgaaaacg cgctacccgc atatcgacac
     7501 gctctctctg ggaatgtcgg acgatatgga agccgccatt gcggcaggta gcacgatggt
     7561 tcgtatcggc actgcaattt ttggtgcgcg tgattactct aaaaaataag gaattaaagg
     7621 aacgccatga atacgttgac tttcctgctt tcaacggtca ttgagctgta taccatggtg
     7681 ctgttattac gcatctggat gcagtgggct cattgtgatt tttacaaccc cttctcacag
     7741 tttgtagtga aggtaacgca gccaattatc gggccactgc gccgcgttat tccggcaatg
     7801 ggccccattg acagcgcctc gctgctggtt gcctatattc tcagttttat caaagccatc
     7861 gtgctgttta aagtggtgac cttcctgcca atcatctgga ttgccggttt actgattctg
     7921 ctgaaaacca tcggcctgct gattttctgg gtcctgctgg tgatggcgat tatgagctgg
     7981 gtaagccagg gtcgtagccc gattgaatac gtgctgattc agctggccga tccgctgctg
     8041 cgcccgattc gccgcctgct accggcaatg ggtgggattg atttctcgcc gatgatcctc
     8101 gttctgctgc tgtatgtcat caatatgggt gtcgcagaag tattacaggc aaccggaaac
     8161 atgctgctgc cggggctgtg gatggcgtta tgagtgccgt aacagttaat gatgacggtc
     8221 tggttttacg gctctatatt cagccgaaag ccagccgtga ttctattgtc ggtttacatg
     8281 gcgacgaagt taaagtcgcc attaccgcgc cgccggttga cggtcaggcc aacagtcatc
     8341 tggtgaagtt tctcggtaag caattccggg ttgccaaaag ccaggtggtg attgaaaaag
     8401 gcgaacttgg ccgccacaaa caaattaaaa tcattaatcc gcaacaaatc ccgccagaaa
     8461 tcgcggcgtt aattaattag gtatcctatg caaaaagttg tcctcgcaac cggcaatgcc
     8521 ggtaaagtgc gtgagctggc gtcgctgctt agcgacttcg gtcttgatat cgtggcccaa
     8581 acagacctcg gcgtagattc cgctgaagaa accggcctga cctttatcga aaacgcgatt
     8641 ctgaaagcgc gccatgcggc aaaagtgacc ggtttaccgg caattgccga cgactctggt
     8701 ctggcggtag atgtgcttgg cggcgcgccg ggcatttact ccgcgcgtta ttccggtgaa
     8761 gacgcgaccg atctaaagaa tctgcaaaaa ctgctggaaa cactgaaaga cgtaccggat
     8821 gaccaacgtc aggcgcgttt ccactgcgtg ctggtatatc tgcgtcacgc ggaagatccg
     8881 actccgctgg tatgccacgg tagctggccg ggcgtgatta ctcgtgaacc agcgggcact
     8941 ggtggctttg gttatgatcc aatcttcttc gtaccttccg aagggaaaac cgccgccgaa
     9001 ctgacccacg aagaaaagag cgccatttcc caccgtggtc aggcattgaa actgctgctg
     9061 gacgctttac gtaatggtta aattaccgcc gctgagtctc tacattcaca tcccgtggtg
     9121 cgtgcagaaa tgcccgtact gcgatttcaa ctctcacgcg ttgaaaggag aagtgccgca
     9181 cgacgattac gttcagcatc tgctttgcga tctggacaac gatgtggctt acgctcaggg
     9241 ccgtgaagta aagacaatct ttattggcgg tggtacgccg agcctgcttt ccggcccggc
     9301 gatgcaaacg ctgctggacg gcgtgcgtgc gcgtttgccg ctggcagcgg atgcagaaat
     9361 tactatggaa gcgaaccctg gtacggtaga agccgatcgc tttgtcgatt atcagcgtgc
     9421 tggtgtgaac cgcatctcta ttggtgtgca gagttttagc gaagacaagc tgaaacgact
     9481 tgggcgtatt catggcccgc aagaagcgaa acgcgcggca aagctggcga gcgggctggg
     9541 gctgcgtagc tttaacctcg atttgatgca tggactaccg gatcaatcac tggaagaggc
     9601 gcttggcgat ctacgccagg ccattgaact gaatccgccg catctttcct ggtatcaact
     9661 gaccatcgaa cctaatacgc tgtttggttc acgcccgccg gtactgccgg acgacgacgc
     9721 gctatgggat atattcgaac aggggcatca gttattaacc gcagcgggtt atcagcaata
     9781 tgaaacttcc gcttacgcca aatccagtta tcagtgccag cacaatctca actactggcg
     9841 ctttggcgac tacatcggta ttggctgcgg cgcgcacggc aaagtgacct tcccggatgg
     9901 gcgcattctg cgtaccacta aaacgcgtca tccgcgtggt tttatgcaag gaaggtatct
     9961 ggaaagccag cgtgatgtcg aagccgcaga taagccgttt gagttcttta tgaatcgctt
    10021 ccgtctgctg gaggccgcgc cgcgcgtgga gtttattgcg tataccgggc tttgcgaaga
    10081 tgtgattcgc ccacagttag acgaggcgat tgcccagggt tatctcaccg aatgtgcgga
    10141 ttactggcag ataacgga
//
LOCUS       DAZFWL020000155        10050 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFWL020000155 DAZFWL020000000
VERSION     DAZFWL020000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.155"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            427..1404
                     /gene="glaH"
                     /locus_tag="ACHKNP_RS21075"
                     /old_locus_tag="pgaptmp_004215"
     CDS             427..1404
                     /gene="glaH"
                     /locus_tag="ACHKNP_RS21075"
                     /old_locus_tag="pgaptmp_004215"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HGU4706279.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1424..2692
                     /gene="lhgO"
                     /locus_tag="ACHKNP_RS21080"
                     /old_locus_tag="pgaptmp_004216"
     CDS             1424..2692
                     /gene="lhgO"
                     /locus_tag="ACHKNP_RS21080"
                     /old_locus_tag="pgaptmp_004216"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HGU4706280.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2715..4163
                     /gene="gabD"
                     /locus_tag="ACHKNP_RS21085"
                     /old_locus_tag="pgaptmp_004217"
     CDS             2715..4163
                     /gene="gabD"
                     /locus_tag="ACHKNP_RS21085"
                     /old_locus_tag="pgaptmp_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4706281.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPMVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4177..5457
                     /gene="gabT"
                     /locus_tag="ACHKNP_RS21090"
                     /old_locus_tag="pgaptmp_004218"
     CDS             4177..5457
                     /gene="gabT"
                     /locus_tag="ACHKNP_RS21090"
                     /old_locus_tag="pgaptmp_004218"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HGU4706282.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5768..7168
                     /gene="gabP"
                     /locus_tag="ACHKNP_RS21095"
                     /old_locus_tag="pgaptmp_004219"
     CDS             5768..7168
                     /gene="gabP"
                     /locus_tag="ACHKNP_RS21095"
                     /old_locus_tag="pgaptmp_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HGU4706283.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7189..7851
                     /gene="csiR"
                     /locus_tag="ACHKNP_RS21100"
                     /old_locus_tag="pgaptmp_004220"
     CDS             7189..7851
                     /gene="csiR"
                     /locus_tag="ACHKNP_RS21100"
                     /old_locus_tag="pgaptmp_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HGU4706284.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7852..8301)
                     /gene="kbp"
                     /locus_tag="ACHKNP_RS21105"
                     /old_locus_tag="pgaptmp_004221"
     CDS             complement(7852..8301)
                     /gene="kbp"
                     /locus_tag="ACHKNP_RS21105"
                     /old_locus_tag="pgaptmp_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HGU4706285.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8385..8543)
                     /gene="yqaE"
                     /locus_tag="ACHKNP_RS21110"
                     /old_locus_tag="pgaptmp_004222"
     CDS             complement(8385..8543)
                     /gene="yqaE"
                     /locus_tag="ACHKNP_RS21110"
                     /old_locus_tag="pgaptmp_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HGU4706286.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8726..9025
                     /gene="ygaV"
                     /locus_tag="ACHKNP_RS21115"
                     /old_locus_tag="pgaptmp_004223"
     CDS             8726..9025
                     /gene="ygaV"
                     /locus_tag="ACHKNP_RS21115"
                     /old_locus_tag="pgaptmp_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGU4706287.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9035..9559
                     /gene="ygaP"
                     /locus_tag="ACHKNP_RS21120"
                     /old_locus_tag="pgaptmp_004224"
     CDS             9035..9559
                     /gene="ygaP"
                     /locus_tag="ACHKNP_RS21120"
                     /old_locus_tag="pgaptmp_004224"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HGU4706288.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(9606..10010)
                     /gene="stpA"
                     /locus_tag="ACHKNP_RS21125"
                     /old_locus_tag="pgaptmp_004225"
     CDS             complement(9606..10010)
                     /gene="stpA"
                     /locus_tag="ACHKNP_RS21125"
                     /old_locus_tag="pgaptmp_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HGU4706289.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2321 a         2578 c         2731 g         2420 t
ORIGIN      
        1 tagaggcgga actctaaata tcaatattcc tgggcgtagc gctcttcttt tagggaaaac
       61 aggagaaccg ccgaactatc tctatttata atttaatgtt atatctgccc cgataaaacg
      121 gggcagataa tatgtttagt ttactaacgg tcattttgca gtgaagccat ttactgtttt
      181 ttatcgacca gataatctgt tctctaatgt taactccccc taacctgttg ctttagttat
      241 tcatttcctg tctcactttg ccttaatacc ctacgttaaa tgttactaat ttgttgcttt
      301 tgatcacaat aagaaaacaa tatgtcgctt ttgtgcgcat ttttcagaaa tgtagatatt
      361 tttagattat ggctacgaaa tgagcatcgc catgtcaccc tacatctcat aagaggatcg
      421 cttctgatga atgcactgac cgccgtacac aataacgctg tcgattcagg ccaggactat
      481 agcggattca ctctcatccc gtcggcgcaa tccccgcgtc tgctggaact caccttcacc
      541 gaacagacga ccaaacagtt tctcgaacag gttgccgaat ggcccgtgca ggcgctggag
      601 tacaaatcgt ttctgcgttt tcgggtaggc aaaattcttg acgatctgtg tgcgaatcag
      661 ctgcaaccat tgctgttgaa gaccctgcta aaccgtgctg aaggtgcgct gttgatcaat
      721 gcggtgggta tcgatgatgt ggcgcaggca gatgagatgg tgaagctggc aacggcggta
      781 gcgcatctga ttggtcgctc aaatttcgat gcgatgagcg gtcagtatta cgcgcgtttc
      841 gtggtgaaaa atgtcgataa ctcagacagc tatctgcgtc agccgcaccg cgtgatggaa
      901 ctgcacaacg acggcacgta tgtcgaagag atcaccgatt acgtgctgat gatgaaaatc
      961 gatgagcaaa acatgcaggg tggaaattcg ttgctgctgc atctcgatga ctgggaacat
     1021 ctggaccact atttccgcca tccactggcg cgtcgcccga tgcgctttgc cgcgccgccg
     1081 agcaaaaacg tcagcaaaga tgttttccat ccagtgttcg acgttgatca acagggtcgc
     1141 ccggtgatgc gctatatcga ccagttcgtc cagccaaaag attttgaaga aggcgtgtgg
     1201 ttgagcgagc tttccgatgc cattgaaacc agcaaaggca ttctttctgt acccgttccc
     1261 gttggcaaat tcctgttgat aaacaacctg ttctggctgc acggtcgcga ccgctttact
     1321 ccgcacccgg atctgcgccg tgaactgatg cgtcagcgtg gctatttcgc ttacgccact
     1381 caccactacc agacgcatca gtaagcgcaa aggaattgag cggatgtatg attttgtgat
     1441 tattggcggc ggcatcatcg gcatgtcgac cgccatgcaa ctgattgatg tttatccgga
     1501 tgcccgcatt gcgttgctgg aaaaagagtc cggcccggcc tgtcaccaga cgggccacaa
     1561 cagcggcgtg atccatgccg gggtctatta cacgcccggc agcctgaagg cgcagttttg
     1621 cctggcggga aaccgcgcca ccaaagcctt ttgcgatcaa aacgacattc gctatgacaa
     1681 ctgcggcaag atgctggtcg ccacgtccga tctcgaaatg gaacggatgc gcgcgttgtg
     1741 ggaacgcacg gcggcgaacg gtatcgagcg cgagtggtta aacgccgagg aactgcgcga
     1801 gcgcgaaccg aatatcaccg ggctcggcgg catttttgtg ccgtccagcg gcattgtcag
     1861 ctatcgcgaa gtcacagcgg caatggcaaa aatcttccag gccagaggcg gtgagattat
     1921 ttataacgcc gaagtcagcg ccctcagtga gcataaaaac ggcgtggtcg tccgaacccg
     1981 tcagggcggc gaatatgaag catcgacgct gattagctgt tccgggctga tggctgaccg
     2041 gctggtgaaa atgctcggcc tcgaaccggg ttttattatc tgcccgttcc gtggcgagta
     2101 tttccgcctt gcgccggagc ataaccagat tgttaaccat ctgatttacc ccattcccga
     2161 cccggcaatg ccatttttgg gcgtgcatct cacccgaatg atcgatggca gcgtcaccgt
     2221 cgggccaaac gcggtgctgg ctttcaaacg cgaaggctat cgcaagcgcg acttctcatt
     2281 tagcgacacg ctggagattt tgggctcgtc ggggattcgc cgggtgctgc aaaaccatct
     2341 acgctcagga ctgggcgaga tgaaaaactc gctgtgcaaa agcggctatc tgcgcctggt
     2401 gcaaaagtat tgtccccggc tttcgttaag cgatctccag ccctggcccg ccggtgtgcg
     2461 ggcgcaggca gtatcgccgg acggcaagct gattgacgat tttctgtttg tcaccacccc
     2521 acgcacgatc cacacctgca atgcgccctc cccggcagcg acatcagcaa ttcctattgg
     2581 tgcgcatatt gtcagcaagg tacaaacgct gttggcaagc cagagtaacc ccggacgcac
     2641 gctgcgagcg gcacgtagtg tggatgcctt acacgccgca tttaatcaat aacctttgaa
     2701 aacaggacgt aacaatgaaa cttaacgaca gtaacttatt ccgccagcag gcgttgatta
     2761 acggggaatg gctggacgcc aacaatggtg aagtcatcga cgtcaccaat ccggcgaacg
     2821 gcgacaagct gggtagcgtg ccgaaaatgg gcgctgatga aacccgcgcc gctatcgacg
     2881 ccgccaaccg cgccctgccc gcctggcgtg cgctcaccgc caaagaacgc gccaacattc
     2941 tgcgcaactg gttcaatttg atgatggagc atcaggacga tttagcgcgc ctgatgaccc
     3001 tcgaacaggg caaaccactg gccgaagcga aaggtgaaat cagctacgcc gcctctttta
     3061 ttgagtggtt tgccgaagaa ggcaaacgca tttatggcga caccattccc ggtcatcagg
     3121 ccgataaacg cctgattgtt atcaagcagc cgattggcgt taccgcagct atcacgccgt
     3181 ggaacttccc ggcggcgatg attacccgta aagccggtcc ggcgctggcg gcaggctgca
     3241 ccatggtgct gaagcccgcc agtcagacac cgttctctgc gctggcgctg gcggagctgg
     3301 cgattcgcgc gggcattccg gctggcgtgt ttaacgtggt caccggttcg gcaggcgcgg
     3361 tcggtaatga actgaccagc aacccgatgg tacgcaaact gtcgtttacc ggttcgaccg
     3421 aaattggccg ccagttaatg gaacagtgcg cgaaagacat caaaaaagtg tcgctggagc
     3481 tgggcggcaa cgcgccgttt atcgtctttg acgatgccga tctcgataaa gccgtggaag
     3541 gcgcgctggc ctcgaaattc cgcaacgccg ggcaaacctg cgtctgcgcc aaccgtttat
     3601 acgtgcagga cggcgtgtat gaccgctttg ccgaaaaatt gcagcaggcg gtgagcaaac
     3661 tgcacatcgg cgacgggctg gataaaggcg tcaccatcgg gccgctgatc gatgaaaaag
     3721 cggtagcaaa agtggaagag catattgccg atgcgctgga gaaaggtgcg cgtgtggttt
     3781 gcggcggtaa agcggatgaa cgcggcggca acttcttcca gccgaccatt ctggtggacg
     3841 tgccggccaa cgccaaagtg tcgaaagaag agacgttcgg ccccctcgcc ccgctgttcc
     3901 gctttaaaga tgaagccgat gtgattgcgc aagccaatga caccgagttt ggccttgccg
     3961 cctatttcta cgcccgtgat ttaagccgcg tcttccgcgt gggcgaagcg ctggagtacg
     4021 gcatcgtcgg catcaatacc ggcattattt ccaatgaagt ggccccgttc ggcggcatca
     4081 aagcctcggg tctgggtcgt gaaggttcga agtatggcat cgaagattac ttagaaatca
     4141 aatatatgtg catcggtctt taactggaga atgcgaatga acagcaataa agagttaatg
     4201 cagcgccgca gtcaggcgat tccccgtggc gttgggcaaa ttcacccgat tttcgctgac
     4261 cgcgcggaaa actgccgggt gtgggacgtt gaaggccgtg agtatcttga tttcgcgggc
     4321 gggattgcgg tgctcaatac cgggcacctg catccgaagg tggtggccgc ggtggaagcg
     4381 cagttgaaaa aactgtcgca cacctgcttc caggtgctgg cttacgagcc gtatctggag
     4441 ctgtgcgaga ttatgaatca gaaggtgccg ggcgatttcg ccaagaaaac gctgctggtt
     4501 acgaccggtt ccgaagcggt ggaaaacgcg gtaaaaatcg cccgcgccgc caccaaacgt
     4561 agcggcacca tcgcttttag cggcgcgtat cacgggcgca cgcattacac gctggcgctg
     4621 accggcaagg tgaatccgta ctctgcgggc atggggctga tgccgggtca tgtttatcgc
     4681 gcgctttatc cttgcccgct gcacggcata agcgaggatg acgctatcgc cagcatccac
     4741 cggatcttca aaaatgatgc cgcgccggaa gatatcgccg ccatcgtgat tgagccggtt
     4801 cagggcgaag gcggtttcta cgccgcgtcg ccagccttta tgcagcgttt acgcgcgctg
     4861 tgtgacgagc acgggatcat gctgattgcc gatgaagtgc agagcggcgc ggggcgtacc
     4921 ggcacgctgt ttgcgatgga gcagatgggc gttgcgccgg atctcactac ctttgcgaaa
     4981 tcgatcgcgg gcggcttccc gctggcgggc gtcaccgggc gcgcggaagt gatggatgcc
     5041 gtcgctccag gcggtctggg cggcacctat gccggtaacc cgattgcctg cgtggcggcg
     5101 ctggaagtgt tgaaggtgtt tgagcaggaa aatctgctgc aaaaagccaa cgatctgggg
     5161 cagaagctaa aagacggatt gttggcgata gccgaaaaac acccggagat cggcgacgta
     5221 cgcgggctgg gagcgatgat tgccatcgag ctgtttgaag atggcgatca cagcaaaccg
     5281 gacgcaaaac tcaccgccga gatcgtggca cgcgcccgcg ataagggcct gattcttctc
     5341 tcctgcggcc cgtattacaa cgtgctgcgc atccttgtac cgctcaccat tgaagacgct
     5401 cagatccgtc agggtctgga gatcatcagc cagtgttttg ctgaggcgaa gcagtagcta
     5461 tgtccgtttg taagagttaa acgttgctaa gggaatgata acgatgcagt aaaaaataat
     5521 aacgatgagg cgatcgacaa agttaaaaac gactgcggac tgtcccctcg ctcctctggg
     5581 gagagggtta gggtgagggg taatcacgcg gctacaccct caccccggcc ctctccctaa
     5641 gggagaggga gaaaactctc ccgctttcgt ttttaagatt ttgttgaccg tctcaattaa
     5701 acgcgtgacc cgacgcggga aatgtcgggg cgctctccca agtgacacac tttcgagagg
     5761 attcaggatg gggcaatcat cgcaaccaca tgagttaggc ggcgggctga agtcacgcca
     5821 cgtcaccatg ttgtctattg ccggtgttat cggcgcaagt ctgtttgtcg gttccagcgt
     5881 cgccatcgcc gaagcgggcc cggcggtatt actggcctat ctgttcgccg gattactggt
     5941 ggttatgatt atgcggatgt tggcggaaat ggcagttgcc acgcccgata ccggttcgtt
     6001 ttccacctat gccgataaag ccattggccg ctgggcgggt tataccatcg gctggctgta
     6061 ctggtggttt tgggtactgg ttatcccgct ggaagccaac atcgccgcta tgatcctgca
     6121 ctcgtgggtt ccaggcattc ccatctggtt attttccctc gtcattaccc tcgccttaac
     6181 tggcagtaat ttattaagcg ttaaaaacta cggcgaattt gagttctggc tggcgctgtg
     6241 caaagtcatc gctatcctgg cctttatttt ccttggtgca gtcgcaatta gcggttttta
     6301 cccttatgcc gaagtgagcg ggatctcaag attgtgggat agcggcggct ttatgcccaa
     6361 cggcttcggt gcggtattaa gcgcgatgtt gatcaccatg ttctcgttta tgggcgcaga
     6421 aattgtcacc attgccgccg cggaatccga cacgccggaa aaacatattg tccgcgctac
     6481 caactcggtt atctggcgta tttctatctt ctatttgtgc tctatttttg tcgtagtggc
     6541 gttaataccg tggaatatgc cggggctgaa agccgttggt tcttatcgct cggttctgga
     6601 attgctcaat attccccatg cgaaattaat catggactgc gtgatattac tttccgtaac
     6661 cagctgtctg aactcggcgc tgtataccgc gtcaaggatg ctctactcct taagccgtcg
     6721 cggtgatgcg cccgcgctaa tgggcaaaat caaccgcagt aaaaccccgt atgtggcggt
     6781 gttactctcc accggagcgg catttttaac ggtggtggtg aactattacg cacctgcgaa
     6841 agtgtttaaa ttcctgatag acagctccgg cgctatcgcc ctgctggttt atttagtcat
     6901 cgccgtttca cagttgcgga tgcgcaaaat tctgcgagca gaaggaagcg aaattcgctt
     6961 gcgcatgtgg ctttatccct ggctcacctg gctggtcatc ggctttatta cctttgtgtt
     7021 ggtagtgatg ctattccgtc cggcgcaaca gttagaagtg atctccaccg gcttattagc
     7081 gatagggatt atctgtaccg tgccaattat ggctcgctgg aaaaagctgg tattgtggca
     7141 aaaaacaccc gttcataata cgcgctgata atgatcagga gtcacaccat gaccattacg
     7201 tctctggatg gctatcgctg gctgaagaac gatattattc gcggtaattt tcaaccggat
     7261 gaaaaattac ggatgagttt gctgacatcg cgttatgcac ttggcgttgg gccgttgcgg
     7321 gaagcacttt ctcaactggt ggcggaacgg ctggtcacgg tggtgaatca aaaagggtat
     7381 cgggtggcgt ctatgtcaga gcaggagctg ctcgatattt tcgacgcccg cgccaatatg
     7441 gaagcgatgc tggtcagtct ggcgattgcc cgcggtggcg atgaatggga ggcagacgtt
     7501 ctcgcaaaag cgcatctgct cagtaagctg gaagcctgtg acgccagcga gaaaatgctt
     7561 gatgagtggg atctgcgtca tcaggcgttt catacagcaa ttgtggcggg ctgtggttct
     7621 cactatttgc tgcaaatgcg tgaacggttg tttgatctgg cggcgcgcta tcgatttatc
     7681 tggctgcggc gaacggtgct ttcggtagaa atgctggagg ataaacacga tcagcaccag
     7741 accctgactg cggcggtact ggcgcgagat accgcgcgcg ccagtgagtt aatgcgccag
     7801 catttactga cgccaattcc cattatccag caggcgatgg ctggcaatta actactcttc
     7861 cggaatacgc aacacttgcc ccggataaat tttatccggg ctttttagca tcggtttatt
     7921 cgcttcgaag attttattgt acagattagc gttaccgtag acctgtttgg aaatggcact
     7981 cagagtgtcg ccagacttaa cggtataaaa ctggctggca gtggctggtg tcgccgtttt
     8041 cacctgatca tcgacactgg caataccgga aatattccca accgcaacaa ggattttctc
     8101 cttcgcctcc tgactcaggc cgtcaccagt gaccgtcgct ttgccgtcgg caatttgaat
     8161 gttcacttta tcggcatcag gtataccggt tttgctcaga tgctcctgca ccttcttcgc
     8221 ctgatcgtct ttatcgtgct gacctgtaac cgcgtcccag agtttttctc cggcatcttt
     8281 cacaaaattg aacagaccca tagctacctc attcgttaac ggaaacactc agaaagtgta
     8341 gcagaggaac gcggcgcgtt aacgccgcgg gggtgagcac aggtttaatc gcgggtttgc
     8401 acccagaacg cgtgaatcaa accggggata tacccaagca gtgtcagcag aatattaata
     8461 atgaacgccc aaccgaaccc tttaccgagc agcacgccga gcggcggcag aatgatggtg
     8521 atgacgattc tccagaaacc catatgtact ccctataaga aaattactca ttgtttaaaa
     8581 agagatttta tctcttaagc gtagtaattt tagcggagcc tgccagtttc agcttacgct
     8641 tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt agagtttact
     8701 taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg ccagtgccga
     8761 acaggcagcg gccttattga aagcaatgag ccatcctaaa cgattgctga ttctgtgcat
     8821 gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg gactgagtgc
     8881 ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatcg acagccaacg
     8941 ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca ttatcgccac
     9001 cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca tttcgccgca
     9061 tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg acgctgatga
     9121 atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc tggaacagtc
     9181 aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg caggtaaacg
     9241 caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa tatttttact
     9301 cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa ataaatctca
     9361 acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat taatcggcgt
     9421 tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg ttggtgccgg
     9481 gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag ataagatgcc
     9541 gtggaaccaa cgaacttgag aagcgacgcc ggacgcgccc tagcagcgac atccggcctc
     9601 agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg agcaattggc
     9661 ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc ggtgaattta
     9721 tatttcgccg gacgcggctg gcgtttttta ccagcgcgtg gtgcagcagc agagctatta
     9781 cctaataact cttccgggtt aattccgtca gccttcatca gctccagcca ggtgctaatt
     9841 ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg tctttcttta
     9901 gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa ttcgcgagcc
     9961 atcgcacgga gagtgcgaat gttatttaaa ctttgtaaca ttacggacat aataaagaaa
    10021 accttttaac gccaaaacaa aaaagtattt
//
LOCUS       DAZFWL020000156         9972 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFWL020000156 DAZFWL020000000
VERSION     DAZFWL020000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.156"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1181
                     /locus_tag="ACHKNP_RS21130"
                     /old_locus_tag="pgaptmp_004226"
     CDS             <1..1181
                     /locus_tag="ACHKNP_RS21130"
                     /old_locus_tag="pgaptmp_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4706290.1"
                     /translation="DNTGAEPADEAGSEQPEETAETVSPVQRLAQLTAELDALHNDRL
                     LVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTIKGQDLAIASLHKH
                     LLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSEFQEKH
                     TVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDKGEM
                     ADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPALLARMEV
                     VPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVTDEIMSRVTRAENGARML
                     ESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGAFTADVEDAQNDESVTKDETVL
                     "
     gene            1178..1921
                     /gene="vasI"
                     /locus_tag="ACHKNP_RS21135"
                     /old_locus_tag="pgaptmp_004227"
     CDS             1178..1921
                     /gene="vasI"
                     /locus_tag="ACHKNP_RS21135"
                     /old_locus_tag="pgaptmp_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HGU4706291.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            1926..3338
                     /gene="tssA"
                     /locus_tag="ACHKNP_RS21140"
                     /old_locus_tag="pgaptmp_004228"
                     /pseudo
     CDS             1926..3338
                     /gene="tssA"
                     /locus_tag="ACHKNP_RS21140"
                     /old_locus_tag="pgaptmp_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
     gene            3447..6881
                     /gene="tssM"
                     /locus_tag="ACHKNP_RS21145"
                     /old_locus_tag="pgaptmp_004229"
     CDS             3447..6881
                     /gene="tssM"
                     /locus_tag="ACHKNP_RS21145"
                     /old_locus_tag="pgaptmp_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HGU4706292.1"
                     /translation="MLILAWIFLLVWIWWKGPMWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQVLLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRNVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            6892..8341
                     /locus_tag="ACHKNP_RS21150"
                     /old_locus_tag="pgaptmp_004230"
                     /pseudo
     CDS             6892..8341
                     /locus_tag="ACHKNP_RS21150"
                     /old_locus_tag="pgaptmp_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            8268..8750
                     /locus_tag="ACHKNP_RS21155"
                     /old_locus_tag="pgaptmp_004231"
     CDS             8268..8750
                     /locus_tag="ACHKNP_RS21155"
                     /old_locus_tag="pgaptmp_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGU4706293.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            8794..9708
                     /locus_tag="ACHKNP_RS21160"
                     /old_locus_tag="pgaptmp_004232"
     CDS             8794..9708
                     /locus_tag="ACHKNP_RS21160"
                     /old_locus_tag="pgaptmp_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706294.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIISSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            9718..>9972
                     /gene="ykfM"
                     /locus_tag="ACHKNP_RS21165"
                     /old_locus_tag="pgaptmp_004233"
     CDS             9718..>9972
                     /gene="ykfM"
                     /locus_tag="ACHKNP_RS21165"
                     /old_locus_tag="pgaptmp_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HGU4706295.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKSFLIKKTKV"
BASE COUNT         2437 a         2642 c         2738 g         2155 t
ORIGIN      
        1 aggataatac cggtgccgaa ccggctgatg aagctggcag cgaacagccg gaagagaccg
       61 ctgaaacagt ttccccggta cagcggctgg cccagctcac tgccgaactg gacgccctgc
      121 ataacgaccg gttgctggtc tccccgcacg tcgataaaaa acagattgcg gcggtgattg
      181 ccgaatggac cggcgtgccg ctcaaccgcc tatcgcagaa tgaaatgtcg gtcatcaccg
      241 acctgacgaa atggctgggt gacaccatca aaggccagga cctggcgatt gccagcctgc
      301 ataagcatct actgaccgca cgcgccgacc tgcgtcgtcc gggacgcccg ctcggtgcgt
      361 tcctgctggc tggccccagc ggtgtgggta aaaccgaaac cgtcctgcaa ctggcagagc
      421 tgctctacgg cggtcgccag tacctgacca ccatcaatat gtccgaattc caggagaaac
      481 acaccgtctc gcggctgatt ggttcgcctc cgggctacgt tggctacggt gaaggcggcg
      541 tactgaccga agcgattcgc cagaaaccct actcggtagt actgctcgat gaagtggaaa
      601 aagcgcaccc ggatgtgctc aacctgttct accaggcgtt cgataagggc gaaatggcag
      661 acggtgaagg ccgcctgatt gactgcaaaa atatcgtctt cttcctgacg tccaacctcg
      721 gctaccaggt aatagtcgag catgccgatg acccggaaac catgcaggaa gtactgtatc
      781 cggtgctggc cgacttcttc aaacctgccc tgctggcgcg tatggaagtg gtgccgtacc
      841 tgccgctgtc gaaagagacg ctcgccacca tcattgccgg gaaactggcc cgcctggata
      901 acgtgctgcg cagccgcttt ggcgcggagg tgattataga accggaagtg acggacgaaa
      961 tcatgagccg cgtcacccgc gcggaaaacg gcgcgaggat gctggaatcg gtcatcgacg
     1021 gcaatatgct gccgccgctc tccctgctgc tgttgcagaa aatggcggcg aatacggcgg
     1081 ttgcccggat tcggttgtcg gcagtggacg gcgcatttac ggcagacgtg gaagatgctc
     1141 agaacgacga gtccgtcaca aaggatgaaa cggttttatg agcaggttgc gcgttttccg
     1201 gcagacagga tggtttatcg cgggcctgat gacgggctta cccgccactg ccgcgcccgc
     1261 agaagcatcg tcaatggccg gtgtggcggt cgccgtcgcc accacgaccc cgccggatgc
     1321 gacagccacg ttacaggcca tgcagtcctg tcggcgggag tccgcggcgc tggagcggct
     1381 tgactgctat gaccgcctgc tggcaccgct gtccccgtca gggtttgacg gggcgctggt
     1441 caaagccggt tttgtgggcg aggcctggac ccgcgccaca gagcaggaaa aacgccgcga
     1501 gggcaacacc acagaactgc tggtgacgca ggtgccgggg gagcgcccga cggtggtgat
     1561 aaccaccccg gctatcggtc atgttccgcc acgcccggtg ctgatgttca gttgcgtgga
     1621 caatatcacc cggatgcagg tggcgctgat gcaccctctg gatgtccatg atatcgccgt
     1681 cactctgaat gccgacaacc gggcattgcg cagccactgg tttgtgcgcg agaacggcac
     1741 cctgctggag tccagccggg ggttatccgg gattgatgaa attaagcaac tgttcggcgc
     1801 aaaaacgctg acggtggata ccggcgcaga caatgccgcc ggaaagctga cttttaacat
     1861 tgacgggctg gcaagggcga ttgctcccct gcgcgatgcc tgccactggg cgggagaata
     1921 aaaatatggc aatggactta cgcgatccga atgtctggat atcgcacctg ctggaaaacc
     1981 tgccggaaga gaaactggca tcggcgctga aagatgacaa cccgaactgg gagtatatcg
     2041 acggcgaaat cgtcaagctg ggatcgctgg cccacagtca actggatatc ccggaactcc
     2101 agcgccgtgg gctggtcatt ctggcttcgg agagtaagga ttttcgtctg ctggcgcatc
     2161 tgctgagaac cctgcaacac gccggtgatc ctcatctcgc attgcgcctg ctggcgctgt
     2221 atgtggagca ttactgggcg gtggctgcgc cgcagaatat ggcgcataaa aaacgctttg
     2281 ccagccaggt cattaaacgc tttgaaacag gtattgaagt tttttcacag aacgccgcca
     2341 cagcgcagcg cgatgcgctg tcaggggaac tggcaaaact ggcgcaatgc tggcagtcac
     2401 ataacgcccc ggaactggca caggctactg atgatctttt tgccctgtac cagcgtgcgt
     2461 ttaatcgtgc ggcaccggct cctgtcccca ctccggcggc ctccggtagt tcaccacaga
     2521 ccaccgccac gtctgaaagc ggcgtgacgc aacccagtgc tccggctccc caaatagtca
     2581 tcgacagtca cgacgacaaa gcctagcgcg acacgctgtt aaaagtggcg gccattttat
     2641 gtgaacgcca gccggattca ccgcagggct accgtctgcg ccgccatgcg ctttggcaga
     2701 acatcaccag tacgccccag gcggaaagcg atggccgtac tccactggct gcggtctccg
     2761 ccgatatggt ggcagattac catgctcagc tcggtagtgc cgatatggcg ctgtggcagc
     2821 aggtggagaa aagcgtactg ctggctcctt actggctgga tggtcactgc ctttctgcac
     2881 agacggcgct gcgtctgggt tacaaacagg tggcagacgc catccgcgat gaggtcatcc
     2941 gcttccttga gcgtctgccc cagcttaccg ggctgctgtt taatgaccac acaccgttta
     3001 tcagtgagca gacgaaacaa tggctggcag cttcgcccga cgccaaagtt gcaccggttg
     3061 cgcaaatcgg tgaggaatcg aaggcagcca gagcctgttt tgctgagcag ggtctggagg
     3121 cggcgctgcg atatctggac atgctacccg aaggcgatcc ccgcgatcag tttcaccgcc
     3181 agtaccttgc cgcacagttg acggaagagg cggggctggt acagcttgcg cagcagcagt
     3241 accggatgtt gttcaggatg ggattgcaga tgatggtggc agactgggag ccgtccctgc
     3301 ttgagcagct agaacaaaaa ttcacggcag aacaataaaa gagaacagga gtttttgtgt
     3361 tcagattacc aacaccccga ctactcagcg gactcaaatc agccctgcga ccggcgatgc
     3421 cccggtttaa agtctctgct ttctggctgc tgatactggc gtggattttt ctgctggtgt
     3481 ggatctggtg gaaaggcccg atgtggacgc tgtatgaaga acagtggctc aaaccactgg
     3541 cgaaccgctg gctggcaacg gcggcgtggg ggattattgc cctggtgtgg cttaccgtcc
     3601 gggtgatgaa gcgcctgcaa cagcttgaaa aaatgcaaaa gcaacagcgc gaggaagccg
     3661 ttgatccgct cagcgtggaa ctgaacgccc agcagcgtta tcttgaccgc tggctgctgc
     3721 gcctgcaacg ccatctcgac aaccgccgtt tcctgtggca gttgccgtgg tatatggtca
     3781 tcggcccggc gggcagtggc aaaacgacac tgctgcgcga ggggtttccg tccgacatta
     3841 tttatgcccc ggagggcgca cgcggcgcag aacaacgcct gtacctcacg ccccatgtcg
     3901 gtaaacaggc ggtgatcttt gatatcgacg gcacactctg cgctcccgct gatgcggata
     3961 tcctgcatcg ccgtctgtgg gaacatgccc tcggctggct gaaggaaaag cgcgcacgcc
     4021 agccgctgaa cgggattatt ctgacactcg atttacccga tctgctgacc gcagacaaac
     4081 gccgccgcga gcatctgtta cagacgctgc gcagccgctt acaggatata cgccagcatc
     4141 ttcactgcca gttaccggtt tacgtggtac tgacccggct tgatttatta cagggttttg
     4201 ccgccctgtt ccagtcactc aacagacagg atcgcgatgc gattctgggc gtcacgttca
     4261 cccgccgcgc ccatgaaaat gatgactggc gaacagagtt gaatgctttc tggcagacat
     4321 gggtggatcg aatgaatctg gcgttgccgg atctgatggt cgctcagact cacacccgcg
     4381 cgtctttatt cagtttttcc cgccagatgc agggaagccg tgaaccgctg gtgtcactgc
     4441 ttgagggtct gcttgatggc gaaaatatga acgtgatgct gcgtggtgtc tatctcacct
     4501 cttcgcttca gcgtgggcag atggatgata tattcaccca gtctgccgcc cgccagtacc
     4561 ggctgggcaa taacccactg gcgtcctggc ccctggtgga caccgcgcct tatttcaccc
     4621 gcagcctgtt cccgcaggta ttactcgcag agcctaatct ggcaacagag agccgcgcct
     4681 ggctgatacg ttcccgtcgc cgcctgacgg ttttctctgc cacaggcggc gtggcagcac
     4741 tgctgctcat caccggctgg catcactatt acaacggtaa ctatcagtcc ggcatcaccg
     4801 tgcttaagca ggccaaagcc tttatggacg tgccgcctcc gcagggggaa gatgactttg
     4861 gcaacctgca actgccgctc ctgaacccgg tacgcgatgc cacactggcc tatggcgact
     4921 ggggcgaccg cagccgtctg gccgatatgg gactgtacca gggacgacgt atcgggcctt
     4981 atgtggaaca gacctatctg caactgctgg agcaacgtta cctgccctcg ctgtttaacg
     5041 ggctggtcaa agcgatgaac gccgcgccgc cggagagtga agaaaaactc gcggtgctgc
     5101 gcgtaatgcg aatgctggag gacaaaagcg gacgtaacaa tgaggtggtg aagcagtata
     5161 tggcaaaacg ctggagcgaa aaattccacg gccagcgcga tatccaggca caactgatgt
     5221 cccatcttga ctacgcgctg gctcatactg actggcatgc agagcgtcag gcgggcgacg
     5281 gtgacgccat cagccgctgg acgccatatg acaagcccgt ggtatcagca cagaaagaac
     5341 tgagcaaact gcctgtctac cagcgggttt accagagcct gaaaacgcgg gcgctgggcg
     5401 ttcttcctgc cgacctcaat ctgcgtgacc aggtagggcc aacctttgac caggtgttta
     5461 catctgccga tgacaacaaa ctggttgttc cacagtttat cacccgctac ggcctgcaaa
     5521 gctattttgt aaaacagcgc gatgaactgg ttgaactgac agcgatggat tcctgggtac
     5581 ttaacctcac ccgcaatgtg aaatacagtg acgccgaccg tgcggaaatc cagcaccagt
     5641 tgaccgagca gtatatcagc gactacaccg ccacctggcg ggctgggatg gacaatctga
     5701 atatccgcaa ttttgagtcc atcggacaac tgaccggggc gctggagcag gttatcagcg
     5761 gcgaccagcc tttgcagcgg gcgctgaccg tgctgcgtga caacacacag ccaggcgtct
     5821 tttctgaaaa actctctgcc aaagaacggg aggaagccct ggcagagccg gattaccagt
     5881 tactcacccg cctcgggcat gaattcgccc cggaaaacag taccctggca gtacagaaag
     5941 acaaagaaag cacgatgcag gccgtgtatc agcaactcac cgagttgcac cgctacctgc
     6001 tggcaatcca gaacgcgcct gtaccaggga aatcggcgct gaaagccgtg cagttacggc
     6061 ttgatcagaa cagcagcgat ccgatattcg ccacccgcca gatggcaaaa acgctgcctg
     6121 ctccgctcaa ccgctgggtt ggcaggctgg ctgaccaggc ctggcatgtg gtgatggtgg
     6181 aggctgttca ttatatggaa gtggactggc gcgacagcgt ggtgaaaccg tttaacgagc
     6241 aactggcaaa taactatccg tttaatccgc gttctgcaca ggatgcctca ctggatgcct
     6301 tcgaacgctt ctttaaaccg gatggcatac tggatacctt ctaccagcag aacctgaagc
     6361 tgtttatcga taatgacctg agtctggagg atggcgataa caacgtcatt attcgcgaag
     6421 atattattgc gcaactggaa actgcgcaga aaatccgtga catcttcttc agcaaacaga
     6481 acggtctggg aacatccttt gccgtggaaa cggtatcgct ttcaggcaat aaacgccgca
     6541 gtgtactgaa ccttgacggt cagttagtcg attacagcca gggccgtaac tataccgccc
     6601 atctggtctg gcctaacaac atgcgcgaag gcaacgaaag taagctgacg ctcatcggca
     6661 ccagcggcaa cgcgccgcgc agtatcagct tcagcgggcc gtgggcgcag ttccgcctgt
     6721 tcggggccgg acaactgacc ggagtacagg atggcaactt taccgtgcgc tttagcgtgg
     6781 acggtggcgc gatgacctac cgtgtgcata ccgacacgga agataacccg ttcagcggtg
     6841 ggttgttcag ccagtttggt ctgtcagaca cactgtactg aggaggcagg gatgaatagt
     6901 aacgtactga cacaaactat cgttaccggc agtgacccgc gcgggctgcc ggaattcagc
     6961 gccatccgcg aggaaataaa caaagccagc cacccgtcac agcctgagct gaactggaaa
     7021 ctggtggagt cgctggcgct ggcgattttt aaagccaacg gtgtggattt acacaccgcc
     7081 acctactata cgcttgcccg gacacggaca cagggactgg cgggattctg cgaaggtgcg
     7141 gaactgctgg cggcaatggt aagccacgac tgggataagt tctggccgca gggcggcccg
     7201 gcgcgtactg aaatgctgga ctggtttaac tcccgaaccg gcaatattct gcgtcagcaa
     7261 atctcctttg cggaatccga cctaccactg atctaccgca cagagcgggc attgcagctt
     7321 atctgcgata agctccagca ggtggaactg aagcgcgttc cgcgcgtgga gaatctgctc
     7381 tattttatgc agaacacgcg taaacggctt ggaccccagc tgaagagtaa cactgagaac
     7441 gccgcacaga ccacggtcag aacgctgatt tatgccccgg aaacacaggc atcttccaca
     7501 ccagaagcgg tagtgcctcc cctgcccggc ctgcctgaga tgaaagtgga agttcgcagt
     7561 ctgacagaga atcccccaca ggccagtgtg ataaatcaag gcagtacggt aagagggttt
     7621 atcgcaggga tcgcctgttc agtggctgtc gcctcagcat tgtggtggtg gcaggtctat
     7681 ccggtgcagc agcaactgtt acaggttaac gacaccgctc agggcgcagc aacggtgtgg
     7741 atggcctcac ctgaactcga aaactatgag cgcaggctgc aacaacttct tgatacctcc
     7801 ccggtacagc cgctggaaac cgggatgcag atgatgcgtg ttgccgacag tcgctggccg
     7861 gaaagcctgc aacagcaaca ggcctcgaca caatggaatg aggcactcaa aacccgcgca
     7921 cagagtagcc cgcagttgcg tggctggttg cagacccgcc aggacttaca tgcttttgca
     7981 gatctagtga tgcagcgcga gaaagaggga ctaacccttt cctatatcaa aaatgtcatc
     8041 tggcaggcgg agcggggact ggggcaggaa acacccgttg agtctctgtt gacgcagtac
     8101 catgatgccc gtgcgcagaa gcagaataca gatgcgctgg aaaaacaaat taatgagcga
     8161 ctcgaaggcg tgttaagccg ctggctgctg ctgaagaata acgtcatgcc agaggcggca
     8221 accggtacca cggccgaaaa ataattattt aattaaagga gattatcatg gcaaatataa
     8281 gttatttatc tttgagtgga gagacacaag ggctaatttc agcaggttgc tcaacgcttg
     8341 attctgtcgg aaacaaagcg cagccagaac ataaagatca gatcatggtt tatgcgttaa
     8401 tgcattccat ctcaagaagt caaaatgtca atcatcatga attaattatc accaaaccgg
     8461 ttgataaatc ctcaccattg ctggcaaaag cacttagtga caatgaaaaa atggcaatat
     8521 gtgaatttat actctaccga accagtaaag cgggtattta ccagccttat tataaaataa
     8581 atctgtcaaa agcccgaata tcaagtattg attttgtcac accgcatgct gtcctggaaa
     8641 aagaactgga accacaggag agaattgcct ttatttatga agatatttcc tgggagcata
     8701 cgttagccgg tacgaatgca atgagtaaat ggcaggatcg tgtacagtaa cttactgtaa
     8761 taacataata aattactatt gcggagagat aaaatggctg gtttaaggaa taacaacaac
     8821 acacagaatg cacagtgggc tgattatgtg ggtgacattc ttcggggggc tcagccgata
     8881 aaccagctag tacctcagca cccttatctt aacgacgtcc cactcattga tgagttaagg
     8941 catcagaaca ctcatcatgt tattctcttg acacttgatg ttgcaaaaaa aatactcagc
     9001 ccaataacat catttgatta cattcatttt atcactacac atccttcagg tataaaagat
     9061 acactcgcct ggttagttaa cgcgggtaaa ttaatgactg agtttgatga caatgggaaa
     9121 attatattta accttaatgc cttaaagtat acaaaagcaa gttactttga aatacttgga
     9181 gagaaatata taaaaatcac aacatccagc ccctggcttt tagaaaagct cggtaaatat
     9241 attttctctt cacgagcgcc tcaagttctg gaactcgcta ttggctggcg aggagcttta
     9301 tctgaatcaa taaaaggagt taagttctgt atctggtttt cagtagcctg gagaaccata
     9361 gaatttatta taagttcaga gcgagatctg gttaattttc ttggcgattt ttcaatggat
     9421 gtggccaaag cagttatagc cggtggtgtt gcaaccgcta ttggaagcct ggcttctttt
     9481 gcctgtgtta gctttggctt tccagtaatt cttgtcggag gagcaatttt actgacaggg
     9541 atagtgtgta cagttgtttt aaatgaaatc gatgctcaat gccatttatc agaaaaatta
     9601 aaatatgcaa ttagagatgg actaaaacgg caacaggaac ttgataaatg gaaaagggaa
     9661 aacatgactc catttatgta tgttcttaac actccacccg tgatatgagg attaaccatg
     9721 cgtttacatg ttaaattaaa agagtttctt tcgatgtttt ttatggccat attatttttc
     9781 cctgcattca atgcttcttt gttttttacc ggagttaaac ctctatattc aataataaaa
     9841 tgctccacgg aaatatttta tgattggaga atgttgattc tgtgctttgg tttcatgtcg
     9901 ttttcctttt taaatattca tgtaatttta cttacgataa taaaatcatt cttaatcaaa
     9961 aaaacgaagg tg
//
LOCUS       DAZFWL020000157         9939 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFWL020000157 DAZFWL020000000
VERSION     DAZFWL020000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.157"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1946)
                     /locus_tag="ACHKNP_RS21170"
                     /old_locus_tag="pgaptmp_004234"
     CDS             complement(<1..1946)
                     /locus_tag="ACHKNP_RS21170"
                     /old_locus_tag="pgaptmp_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4706296.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLTRA
                     RVNQQNTENAAEKTGAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLET"
     gene            complement(2040..2231)
                     /locus_tag="ACHKNP_RS21175"
                     /old_locus_tag="pgaptmp_004235"
     CDS             complement(2040..2231)
                     /locus_tag="ACHKNP_RS21175"
                     /old_locus_tag="pgaptmp_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4706297.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(2228..2416)
                     /gene="dicB"
                     /locus_tag="ACHKNP_RS21180"
                     /old_locus_tag="pgaptmp_004236"
     CDS             complement(2228..2416)
                     /gene="dicB"
                     /locus_tag="ACHKNP_RS21180"
                     /old_locus_tag="pgaptmp_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4706298.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            2990..3175
                     /locus_tag="ACHKNP_RS21185"
                     /old_locus_tag="pgaptmp_004237"
     CDS             2990..3175
                     /locus_tag="ACHKNP_RS21185"
                     /old_locus_tag="pgaptmp_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706299.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITKP
                     NQYQSDLLNKELNAARD"
     gene            complement(3362..3751)
                     /locus_tag="ACHKNP_RS21190"
                     /old_locus_tag="pgaptmp_004238"
     CDS             complement(3362..3751)
                     /locus_tag="ACHKNP_RS21190"
                     /old_locus_tag="pgaptmp_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706300.1"
                     /translation="MEFKDLLKEIQEIAAHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
     gene            complement(3763..3891)
                     /gene="ydfB"
                     /locus_tag="ACHKNP_RS21195"
                     /old_locus_tag="pgaptmp_004239"
     CDS             complement(3763..3891)
                     /gene="ydfB"
                     /locus_tag="ACHKNP_RS21195"
                     /old_locus_tag="pgaptmp_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HGU4706301.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            complement(3893..4048)
                     /locus_tag="ACHKNP_RS21200"
                     /old_locus_tag="pgaptmp_004240"
     CDS             complement(3893..4048)
                     /locus_tag="ACHKNP_RS21200"
                     /old_locus_tag="pgaptmp_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4706302.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(4325..4612)
                     /locus_tag="ACHKNP_RS21205"
                     /old_locus_tag="pgaptmp_004241"
     CDS             complement(4325..4612)
                     /locus_tag="ACHKNP_RS21205"
                     /old_locus_tag="pgaptmp_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4706303.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(4612..4803)
                     /locus_tag="ACHKNP_RS21210"
                     /old_locus_tag="pgaptmp_004242"
     CDS             complement(4612..4803)
                     /locus_tag="ACHKNP_RS21210"
                     /old_locus_tag="pgaptmp_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HGU4706304.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(4831..5232)
                     /locus_tag="ACHKNP_RS21215"
                     /old_locus_tag="pgaptmp_004243"
     CDS             complement(4831..5232)
                     /locus_tag="ACHKNP_RS21215"
                     /old_locus_tag="pgaptmp_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4706305.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            5341..5613
                     /locus_tag="ACHKNP_RS21220"
                     /old_locus_tag="pgaptmp_004244"
     CDS             5341..5613
                     /locus_tag="ACHKNP_RS21220"
                     /old_locus_tag="pgaptmp_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4706306.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            5597..6022
                     /locus_tag="ACHKNP_RS21225"
                     /old_locus_tag="pgaptmp_004245"
     CDS             5597..6022
                     /locus_tag="ACHKNP_RS21225"
                     /old_locus_tag="pgaptmp_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4706307.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(6229..6684)
                     /locus_tag="ACHKNP_RS21230"
                     /old_locus_tag="pgaptmp_004246"
     CDS             complement(6229..6684)
                     /locus_tag="ACHKNP_RS21230"
                     /old_locus_tag="pgaptmp_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706308.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            6763..7878
                     /locus_tag="ACHKNP_RS21235"
                     /old_locus_tag="pgaptmp_004247"
     CDS             6763..7878
                     /locus_tag="ACHKNP_RS21235"
                     /old_locus_tag="pgaptmp_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4706309.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSAYALKADSGA
                     VQQVMTARPKQSHQLQQPEADSAIQREADRVVPENTGLSVGRVDYPDVFEQVWREYPL
                     RAGANPKKSAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGP
                     DRNFENPWLLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            7885..8631
                     /locus_tag="ACHKNP_RS21240"
                     /old_locus_tag="pgaptmp_004248"
     CDS             7885..8631
                     /locus_tag="ACHKNP_RS21240"
                     /old_locus_tag="pgaptmp_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4706310.1"
                     /translation="MKNIAAAGVLERIRRLAPPASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMVKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            8653..9423
                     /locus_tag="ACHKNP_RS21245"
                     /old_locus_tag="pgaptmp_004249"
     CDS             8653..9423
                     /locus_tag="ACHKNP_RS21245"
                     /old_locus_tag="pgaptmp_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4706311.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRKGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            9439..9861
                     /locus_tag="ACHKNP_RS21250"
                     /old_locus_tag="pgaptmp_004250"
     CDS             9439..9861
                     /locus_tag="ACHKNP_RS21250"
                     /old_locus_tag="pgaptmp_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4706312.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPSVVDPDLIWSLPDG
                     EIRRYDRRLNIICSECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT         2505 a         2229 c         2545 g         2660 t
ORIGIN      
        1 gtttcaaggt caatcatcag atggtgccac aacctgctgg tggatgagat ttcatgatga
       61 ccgttcactt taattaaggg atttgctgtt tcgccagttt tattatcgct ggcgtgatac
      121 tgatcgctgc cagtgttctc cttgtgcaga tgttcagtgc cttccatttc ctccggatca
      181 tttttctgag cttcagcctg attctcttca tcgaatgttt ccaggtaggt tgcgttcccc
      241 atcaccgcac cacagtcagg acagttgccg ccaccggtct gaccgcatgc ggtgcaaact
      301 ttttccagtt cctgttgcac tgctggttct ggttgttgtt cttctggccc gttttgttgc
      361 gtatccgggc tgttttgttc cgcttctggc tcattttgtt tcacgttggg ctgattctgg
      421 tcctcagcgt cgcgactctg gatccctttc acccatttcg gatcattcgg gtcgctaatc
      481 ccttcaacaa attcaccacg tgatgcagca agcaatttat cggcgtcagg ctggctgata
      541 ttggctgcct gcataatttt gtttacttcg tcagcggtaa cttttaccgg ctctgattgt
      601 tcggaatctt cagcggtatc cacattttgc ggtaagcccg tgtatgtgcc attttttcgg
      661 gcaaaatatt cttcttttga gatttcggta ccgccggcag ccagcgcctt gtccagacca
      721 gaaagtttgt ttgccctgcc gtatttttcc ccgcccttat cggtaaagac gaaataaaat
      781 ggtccttcac gctctacaga tggttcagct tccaccagga tttcattttt ttgagtatcg
      841 gattctgccg tctccactgg agcagtttgt gctgctgacg gctggagagc atcagtagag
      901 ctctggtctg tttcttcatg ttcaaacacg ccctttgtcg tcaggtattc actgatatat
      961 ttgttcagtg caacgggatc tttgtgaata tcgatcggac gttcgcgaac aaggccaaaa
     1021 atagtctgac ggtcatagcc aagtgcttcg ggctgtttgc gcattgatgc tgagatacgc
     1081 ttccagtctt cgcgatcctt gtcgataacc tcatttgtcg cccagcgatg gatggtaccg
     1141 tcaatgtttc ctgcatccac atcaccgggc cagagggcgc aggccagttc attgtcgagt
     1201 gttttccatg tctgtttgta ttcgcggcga atggcaacag tgacagcccc ggttttttca
     1261 gcagcgtttt cagtgttctg ttggttgact ctggcgcggg tgagatcaac aacagacgtg
     1321 tattttccgg tttccttgcg ttcaccttcg cgacgttttt tccagatgcg catctctgcc
     1381 tgaatttcag gccatttagc gccaggctca catttatgct taacccaccc gatggcatgc
     1441 agtttaagtt caggatacat ggcgttaact tctggcattt tcatcaacgc ttcaacgata
     1501 tgtccgccga atgttgccat gtcttcctgt agtaattcct gtgcgctaat caccatatca
     1561 acggtgatgt tttcgcatgt gtcgaattta accaggactg cgttctgtac ttcaggggac
     1621 agcttgtcaa aattgacgtt catcggatcg gattctggtt cgaccggtac aaaggaagct
     1681 gactcctcat cccagcggtt ttcctgcata tattcggtat cccaggaatc gagggcaggg
     1741 cggggtatgc cgggtttatc ctcgcagaca ataaatttat aagcgcagtc ctgagctgcc
     1801 gggaattgct ccagaaattg ccagtgaaat tttgcgcgtg cgcgacgctc atcaccggct
     1861 tcaatggcag tggccaccgc aacagcgcta tcttctttta tggcctgttc gtcaggaata
     1921 gcagcgcaaa taaagatttt actcattgtg ttttaacctc attacagatt taagggtgaa
     1981 caaatccctg ccattgctgg catataaaaa tgaaaccgga tattaattac ggtgctgttt
     2041 taaagtcctg ccggtatttc gttattatta gtgtgagtag ttttatctac cggataacag
     2101 ttaccgggaa ttttttgttc tgctgctgca gccatgcatt ctttcattga accgtataag
     2161 ccagtcacca gctcaagaga ttcgccggaa acaagataaa ctgtcagaac gagtgcaaat
     2221 gttgtattca ttgtttatat cctttttgca gcaggtccag acgagccagc attgaaggaa
     2281 tgcatacttc atttaacagg tcctgctcga gttttctctg cttaatggcg tcttcaataa
     2341 atgttttgtc tccagtgata acgccaattt cgaaacgaag ttcagacgtg ctggcattac
     2401 atgataactt ttccattatc gcgtcctcaa caatgaattt tgtgatgcgg tgcctggtgc
     2461 ctccaggtga cgttaaccag ttaacaatta acgccggata cagagcattc tcgttactca
     2521 gcaaatgacc gctttaccga tttaactgtt ccgcgtgcgc tgagccgcat tcaccgcatc
     2581 acaaaattca cttttaaaaa agggcggcag agcagtcacg gagtaaaact gataccgcca
     2641 aatgtcacca gaatattgat aacagagggc gttgcagcgg ggttgtcact taagcgtatg
     2701 gtcaacctga caacccggtg tcctcaacgg ggaaagaata accccgccat acttaccgcc
     2761 gcgccatttc gcggattgcc acaaccggaa gcgcacggtc gaattaaatt taacgacggc
     2821 tctaagggag aaggacttcg ccgtgcactt tcgtgttatg ccctgacttt tcagggatat
     2881 atcctttcag taaactgtca gtgccggatt cttatctgtg tccggcgcac gaccacacgt
     2941 agcagcgtgt tggtctccat ttttaattca gcccttaatg gaggataaaa tgccaaacaa
     3001 accatgccct gcctgtaacg ctctttctgg ctttttagag aagggcggtt attacatttt
     3061 taactgtcca gaacatgttg aatttcatat atcaaaactt gacagtatca ttacaaagcc
     3121 aaatcagtat cagtctgatt tactgaataa agagttaaac gccgcccgag attaagggcg
     3181 tagtggccca ttaaaaatat tgcaaagacc aactattgtt gaactggatt aaaccagtaa
     3241 ttctctattg ctcttattgt taccatcgga tgattgccgg gggttatttt aagttcggga
     3301 ggaaaaggct ctccatcagc agccatcatt gagtatgtga aatccgccga cgcagtccct
     3361 ttcatgataa ttccgaattt ttcaagtaat gagagcagtt ccggtaatcc tgtgtcatct
     3421 gaataacttt cctcatagaa agaagcgatt gccgcaggag atgacagttc tcgctctgca
     3481 atttttttgt agttttccag aattcttttt ttgaatactt ttcctcgaca tgaaaaccca
     3541 tcaccgaggc aatccgcttt gcagtttctt cagtattctg gttgtttgtt accgaaacag
     3601 agtacaatcc ggtaaacgca tcgcgcacat tacgagccat attatcaatg tatttcttcg
     3661 ttgcagattc taattcaact tcgttcagac gttggtgaag tgcatgtgct gcaatttcct
     3721 ggatttcttt tagtaaatct ttaaattcca tctttaactc cgttagtcgt tagattttgc
     3781 cgaactggaa agcgcctgtt taaactcact gaagctgaga gcttcctcgc cttcggcaag
     3841 gccttcgaag tattcttcgt aagccttttc catgattgtg tcgaaatcca tatcactcac
     3901 ctgagtttct ttccagccag cgacgggcac cattttcggt tttaaacgtt ttgcttttgg
     3961 tatacgtcat cgcggtgaat gtaccgtcct ggttgggaaa cacgccgtac accagagatt
     4021 cgttgttgcc aagatcgata gtatccatgt tgacctcatt tccccttaac gccgggtagc
     4081 ggaacaaaaa cctgctgcat agttattaaa gttgaaccct gccgtcatgt tcatacgcct
     4141 cgggctggct acttaccccc tgaccactgc ttggtaactc gaagtattgc ccggcgttct
     4201 gtggggcggg gtgggtggtt ggtgtatgta atctacaatt aaaaactgtt ttgtgtcaac
     4261 agtttttaat tgttgttttg ggcaaaaaaa tccctcgaaa gagggagtat gaaaattgtt
     4321 caactcagat agagaaggga aattgtcgcc gagaatgtgt cacgcttaca atctcaatgc
     4381 ttgaagcagc tactctgtac aggattatgt agttagggtg ggtcacgatc tctctcaatc
     4441 cagaaacccg ttcgcttggt ggatataagt acggatgctc agatagaggt aataccgatg
     4501 tttcaatgcg tatttttagt ctacgtgctg ccggtgggtt ctccttggcg atgtaagtta
     4561 tgatctggcg caaatcatcg cgagcagacg gtagccataa aatgggtaac attactcgct
     4621 cttgttagtt acagcaattt gagcaataag attttccatt tcagccatta cctcgtcatg
     4681 tggaattgcg gggcgagtgt ctgcaaggct tgacgttact ttagtgcgca accattcgtt
     4741 gtaactgttt tcttgttcgg tagtttcgaa ttctgaaact atcggagaaa gggctgtacc
     4801 catggcataa ctcctcttct tgtactgtgg tcacgcccgg cggctttttt gtgccgccag
     4861 ccacctagca atggtttctt ccattgattt tttcttgtct ttgatttctt gaagcatttt
     4921 ttcttggtct tcctcaggaa acgcactaaa agcttggagc agttcgcgtt ggcgaggacc
     4981 aattttcatc gtgtcagggg tgaaaatttg ctcacactct tcaggaggca ataaaaacca
     5041 atgcaatgga tgccctgaaa cctcaaccag tttatccaaa cttgaggctt taggtgtagc
     5101 cttaccgctg acccattgtt gaacagtttg ttgtgtcaca ccaattctac gggcaagctc
     5161 agcctggctc catccagttt cctgaagaag cttgctgatt ctgtacatag atacttctag
     5221 ggcgctcatc attattcaat tttacaggta aatactgtta aaagcatcac aataaaaaac
     5281 tgttgattgc atacagtttt ttattgtagg ctttgcttat agtttttaga ggagggcaaa
     5341 atgctagata gcactcgcga aaaaattagg cagaaataca ctcaggctga aataggtcgt
     5401 tatatggggg tcgctcaaca gactgtttgg caatggttta gctttggcgt tcccccaaag
     5461 caggtaattc cgttatgcca actaatgaag tgggaagtta ccccgcatga aatccgccca
     5521 gatatttatc ctaacccaac cgacggttta cctgttggat gtaaggttaa cacatcaaat
     5581 gcgccggagt tgattcatga aaatcaagca tgaacacatc cgcatggcga tgaatgcctg
     5641 ggcgcatccg gacggcgaaa aagtaccggc tgcgaaaatt accaaagcgt atttcgagct
     5701 gggaatgacg ttcccggaac tgtatgacga cagccatccg gaagccatgg ctcgcaatac
     5761 tcagaaaatt ttccgctggg tggagaaaga cactcctgat gcggttaaaa aaattcaggc
     5821 gttgttacca gcgatcgaaa aagcgatgcc gcctccgctg gtggcccgaa tgcgcagcca
     5881 cagttccgct tattttcggg agttggtaga gacgaaggaa cggctggtga aagatattga
     5941 tgatttcgtt gcatcagcga tcgttctgtt cgatcagatg aatcgtggtg gcccggcagg
     6001 aaacactctg gctgtgcatt aattgggtaa taaatatgag taatgacaaa aaattgacac
     6061 tgagcgttta cgaaaacagt ccgcacatct ggcgtggcgg tttatctgat gtggagctgg
     6121 cagagtggtt gatacataaa gctaatgcgc tgctctggcg tttgtcagct agagaacagc
     6181 gcaaggaaac cagaataaag ctggctgatg cagaagcgtg tgccgggctt attgaggatt
     6241 atacaaatct tggtatttct tcagcagaga gtgatcccat tcagcctctg agcagggagt
     6301 caatccagca cgctggttgt atggcacatc ttgtaactgc tcgtcaacat gaggtgggta
     6361 ttggatcact tccggtggga tattcgctga ttccagagct ggttgaagca agaaaatcag
     6421 ttcagaaaaa gagagatgac gcacttcaat tattgagaga gcactatggc gcgataccag
     6481 aatgcgaaca gcatcgatac cctgaaggtt atgaatggat gcagtctctt tttgaagttc
     6541 gctaatcaat atgtcgagac gaaggtatgt ttcggcgcgc agccaggctc tgtaatccgg
     6601 gagcatttcg gggctgttac accagcggtt tgttgctgca acatttaata catgagcctg
     6661 ataaaggctt ttcaaaaaat acatgtcgaa cctcctctgg ttctgtcgat tgggaaccac
     6721 agattatatc cggaggaagg ttcggcacca gatgaggtag ccatgcgtga ttacgcaaaa
     6781 gtttctccgc gattctggct gggagaaacg gggagagaac ttagaaaggc gggtgcagaa
     6841 gcgcaagttg ttgcttttta cctgatgaca tcccctcacg caaatatgct gggtttgtat
     6901 tacctgccag ttttatacct tgctcatgaa accgggcttg gtctggaagg ggcttcaaag
     6961 gggcttaaaa gggctgttga agctggtttt tgtagctatg accatgatgc agagatggtc
     7021 tgggtccatg aaatggcagc ctggcaggtt ggggaaacgt tgaagcctgg cgataaccgt
     7081 tgtgcaggtg tcaggaatga gtatgcatca ttacctgaaa acgcttttct gtcagtgttt
     7141 tacgacagat ataaaacgga tttccatctg gatgtgaggc ggaataatag ccgaaattcg
     7201 gtaaggggct tcgaaggggc ttttaagggg cttcgaagcc aagaacagga acaggagcag
     7261 gagaaagaac aggaacagga caaaaacact atggttcatg gcaaaaaaaa caccacgaac
     7321 caggcagggg atgttcagac cgtcaatcct ggtcagccag caggcacgac accggaagcc
     7381 gattcagcgt atgcgctgaa agccgattcg ggcgctgtgc agcaggtgat gaccgcaagg
     7441 ccgaagcaat cacaccaact gcagcagcct gaagccgatt ccgccattca gcgggaagcc
     7501 gatcgggtag tcccggaaaa caccgggctg tctgtgggac gagtggatta tccggatgtg
     7561 ttcgaacagg tctggcggga gtacccgttg cgtgccggag caaacccgaa gaaatccgca
     7621 ttcagtgcct ggaaggccag attgcgcgag ggggtgccac cagagaccat gctggatggt
     7681 gtgaggcgtt acgcgagata cctggcggcg accgggaaag cgggaacgga atttgttcag
     7741 cgagcgacga cgttttttgg gccggaccgg aattttgaaa acccctggtt gctcccggta
     7801 agcggcacga acaaccagcg ttgtgtgaat catatttctg aaccggatac cgaaattccg
     7861 ccgggattca gggggtgatg tggcatgaaa aacattgcgg cagccggggt tcttgaacgt
     7921 attcgcagac ttgcaccacc ggcgtcggtt ccaccgtacc ggacggtgga ggagtggcgg
     7981 gaatggcaac ttgctgaagg acgaaaacgc agcgaggaga ttaaccgcca gaatcaccag
     8041 ttgcgggtgg aaaaaatcct gaatcgttcg ggcatccagc ctctgcacag caaatgctcg
     8101 tttgcgaatt atcaggtgca gaacgacggg caaaaatacg cgctgagcca ggccaaatcc
     8161 atagctgacg aactgatgac cgggtgcacg aattttgtgt tcagcggtaa aaccggcacc
     8221 gggaaaaatc accttgcagc ggcgatgggc aaccggctga tggtgaaggg gcgcagcgtg
     8281 attatcgtca ccgtgtctga cgtcatgagc gtgttgcatg acagctacga caacggcaaa
     8341 tccggggaaa aatttttaca ggagctttgc ggggttgatt tgctggtcct ggatgaaata
     8401 ggcgttcagc gggagacgaa aaacgagcag gtggtattgc accagataat tgatcgccgg
     8461 acagcatcac tgtgcagtgt cgggatgtta acaaacctga atcatgccgc aatgagtacg
     8521 cttcttggtg agaggattat ggaccgcatg accatgaacg gtggtcgatg ggtgacgttt
     8581 aactgggata gctggcgtcc aaatgtcagc aatatgaggg ttgtgaagta attttgtccg
     8641 gaggaaattt taatggaaac cgtatctgac gcactgaaag cactgaaaaa agcctcttca
     8701 catgtggtgg cagctcgcct tggaatcagt cgtgaagagg ctgtcaacga gctgtgggaa
     8761 ctcaaaagaa aaggcgtcgt tgataaaact ggtcacacct ggtttctggc tggcgaaggt
     8821 gaatcccggg taaccgaaga gcggccagta aaatctgaag cacaggatat gctgaccggg
     8881 gaggtcgaac aaaaagttac cgcagacatg atgattgagt ttatcggtca ggatggggct
     8941 aaaacgtgtg aggaactggc gggtaagttc ggtgtcagta ctcgcaaggt tgcttccacg
     9001 ctggcggtgg taaccgcaac ggggcggctg gcacgcgtta atcagaacgg taaatttcgt
     9061 tactgcatgc cgggcgataa tttaccagca gagccgaaag ccgcgctggt aacggaaagt
     9121 gatggtaagg cctttcctca gccagcaggt gctgcgttac cagtccggga agccgcaaca
     9181 caggaagaaa ttaaaacaga aactgtggcg gacattgtgc agccgttgcc atcgtttacc
     9241 gaaacgcaag cagatgagct gatttttccg tcccttcgca gggcaaacct ggcgctgcgc
     9301 agggcgaaaa gtgatgttca gaagtgggag cgagtctgcg ccgcgctgcg ggagctgaac
     9361 aagcaccggg atattgttcg acagattact gattcttccc gccgtgttgt atcggaaaag
     9421 tgattgccgg aggcgcttat ggcaaaagta tttacacaag aagagcggga aaaaattaaa
     9481 gggcaggttg ttgaactagt acgccggagt gggcgcgaga cgttacggca actggaagcc
     9541 aagacaggtg cgacaagata tctgatgagc gttctcgcca gagagctggt tgccagtggc
     9601 gatgtataca actctggtta cgggttattc ccgtctgaac aggctcgtaa ggactggcaa
     9661 aatgcccgca aaaaactatc tagggcaaag gtgaagaaac catctgtggt tgatccggat
     9721 cttatctggt cattaccaga cggagaaata cgccgctacg acaggcgtct gaacataatc
     9781 tgtagcgagt gccggaagag cgaagctatg cagcgtgtac tggctttcta tcagggtaat
     9841 tttcagaagg tgctgttgtg agccaaatta acaatcggaa cttcgtgaag agaaagcata
     9901 atccaaatct gaataattaa gttcagcact gtaaataaa
//
LOCUS       DAZFWL020000158         9935 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFWL020000158 DAZFWL020000000
VERSION     DAZFWL020000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.158"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(431..506)
                     /locus_tag="ACHKNP_RS21255"
                     /old_locus_tag="pgaptmp_004251"
     tRNA            complement(431..506)
                     /locus_tag="ACHKNP_RS21255"
                     /old_locus_tag="pgaptmp_004251"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(471..473),aa:Asn,seq:gtt)
     gene            complement(607..2094)
                     /gene="yeeO"
                     /locus_tag="ACHKNP_RS21260"
                     /old_locus_tag="pgaptmp_004252"
     CDS             complement(607..2094)
                     /gene="yeeO"
                     /locus_tag="ACHKNP_RS21260"
                     /old_locus_tag="pgaptmp_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HGU4706313.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAVAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            2255..2330
                     /locus_tag="ACHKNP_RS21265"
                     /old_locus_tag="pgaptmp_004253"
     tRNA            2255..2330
                     /locus_tag="ACHKNP_RS21265"
                     /old_locus_tag="pgaptmp_004253"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2288..2290,aa:Asn,seq:gtt)
     gene            complement(2368..3318)
                     /gene="cbl"
                     /locus_tag="ACHKNP_RS21270"
                     /old_locus_tag="pgaptmp_004254"
                     /pseudo
     CDS             complement(2368..3318)
                     /gene="cbl"
                     /locus_tag="ACHKNP_RS21270"
                     /old_locus_tag="pgaptmp_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
     gene            complement(3420..4337)
                     /gene="nac"
                     /locus_tag="ACHKNP_RS21275"
                     /old_locus_tag="pgaptmp_004255"
     CDS             complement(3420..4337)
                     /gene="nac"
                     /locus_tag="ACHKNP_RS21275"
                     /old_locus_tag="pgaptmp_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HGU4706314.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4663..4738
                     /locus_tag="ACHKNP_RS21280"
                     /old_locus_tag="pgaptmp_004256"
     tRNA            4663..4738
                     /locus_tag="ACHKNP_RS21280"
                     /old_locus_tag="pgaptmp_004256"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4696..4698,aa:Asn,seq:gtt)
     gene            complement(4782..5729)
                     /gene="ldtA"
                     /locus_tag="ACHKNP_RS21285"
                     /old_locus_tag="pgaptmp_004257"
     CDS             complement(4782..5729)
                     /gene="ldtA"
                     /locus_tag="ACHKNP_RS21285"
                     /old_locus_tag="pgaptmp_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4706315.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDSSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMFYERW"
     gene            complement(5791..6870)
                     /gene="cobT"
                     /locus_tag="ACHKNP_RS21290"
                     /old_locus_tag="pgaptmp_004258"
     CDS             complement(5791..6870)
                     /gene="cobT"
                     /locus_tag="ACHKNP_RS21290"
                     /old_locus_tag="pgaptmp_004258"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HGU4706316.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCAAHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6882..7625)
                     /gene="cobS"
                     /locus_tag="ACHKNP_RS21295"
                     /old_locus_tag="pgaptmp_004259"
     CDS             complement(6882..7625)
                     /gene="cobS"
                     /locus_tag="ACHKNP_RS21295"
                     /old_locus_tag="pgaptmp_004259"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HGU4706317.1"
                     /translation="MSKLFWAMLSFITRLPVPRRRSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7622..8167)
                     /gene="cobU"
                     /locus_tag="ACHKNP_RS21300"
                     /old_locus_tag="pgaptmp_004260"
     CDS             complement(7622..8167)
                     /gene="cobU"
                     /locus_tag="ACHKNP_RS21300"
                     /old_locus_tag="pgaptmp_004260"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HGU4706318.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(8319..8492)
                     /locus_tag="ACHKNP_RS21305"
                     /old_locus_tag="pgaptmp_004261"
                     /pseudo
     CDS             complement(8319..8492)
                     /locus_tag="ACHKNP_RS21305"
                     /old_locus_tag="pgaptmp_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(8471..8659)
                     /locus_tag="ACHKNP_RS21310"
                     /old_locus_tag="pgaptmp_004262"
                     /pseudo
     CDS             complement(8471..8659)
                     /locus_tag="ACHKNP_RS21310"
                     /old_locus_tag="pgaptmp_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(9656..9935)
                     /locus_tag="ACHKNP_RS21315"
                     /old_locus_tag="pgaptmp_004263"
                     /pseudo
     CDS             complement(9656..9935)
                     /locus_tag="ACHKNP_RS21315"
                     /old_locus_tag="pgaptmp_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2749 a         2629 c         2102 g         2455 t
ORIGIN      
        1 tatggcagat ttctttaatc tcatataatt cttataaaaa atataatgtt taactcgtca
       61 tattgcttat aaaaatcccc gtccccagag aaataatatt tattaaaatg gcaggtctgc
      121 tttttctgat taccgaagac aaagtggcaa ttacgtatag tttcccgata aagacgcgac
      181 agctacatcc cgcataaggc atttttctct ttatctttgt acggtacttc atggaacaga
      241 atttttgacc ttgtgaatcg tgatgtctgt tggggaggag caatttgctc actgaagcgt
      301 gagactcgat taagcgcacg aaacacagaa atcaaaaaac ccggccactt ttttacaaac
      361 tgaccgggta aaaataattt ttatttttta actgccttga gactcataga gatgtctcaa
      421 aactaaaatt tggctcctct gactggactc gaaccagtga catacggatt aacagtccgc
      481 cgttctaccg actgaactac agaggaatcg tgagaacgag gcgaatatta gcgatgccca
      541 cccacaatgt caaagcctgt tttttaaatt tgaaatcgtt tgctgaaata atctgcattt
      601 tgttgtttat tccgacacaa ctggcttttt ttcacacttt tgcggttcgg gtcgagggta
      661 tttccatagc caacgtccag taaccattcg ccagtaaaac agcacggccc gcacagccca
      721 gtcggcaaac attcccatcc agacaccaac cacaccccag ccaagcatga tgcccagcac
      781 ataaccgacg acaacccggc aaccccacat gctcaacata gaaacccaca tagcgtaacg
      841 ggcgtcacga gcccccttaa atccagcggg tagcacccat gaggcggacc aaataggcat
      901 aaataaagca tttagccaaa tcagaatcac aacgacatgt ttaacctgtg gatcctgggt
      961 gtaaaacgat gccataaccc cggcaaaggg agccgttagc caggcgatgg ccgttaatcc
     1021 aagagtggac agccagaaga catgccgcaa ctgaatctct gcttgcgcta tctgccctac
     1081 ccctaacctt cggcctgtaa tgatcgtaga agcagagccg agcgcacttc cgggtaagtt
     1141 gataagagcc gcaattgaaa acgcgataaa atttccggca ataacactgg tccccatccc
     1201 ggcaacgaac atttgggtta ataaccgacc actggtaaat aacactgatt cgacactcgc
     1261 tggaatacca atccccatga cttcccagat aatgctaaaa ttcagcggtt taaaatagct
     1321 ctttaacgaa atccttagcg caggattaaa accaatcgcc agcacccaca aaattgcaac
     1381 tgcgccaata taacgagaaa tggttaaacc caacccagcc ccgacaaatc ccagtcccgg
     1441 ccaggagaaa aggccgtaaa tcaatatgcc gctaataata atattaagaa tattcaggct
     1501 accgttaatc agtagcggta ttttcgtatt ccctgcacca cgaagtgccc cgctaccaat
     1561 caaagtgatg gcaactgctg gataactgag taccgtcagc tccagataag tcaacgccag
     1621 tgctttaact tctgtcgtgg catcacccgc gacgaaatca ataatttgtt cgccaaaatg
     1681 atgaataagc gttgccaaca gtacggcaaa caacgtcatg atcaccaatg actgccgcgt
     1741 cgccaccctc gctcgtcgtc gatcccgctt accgagacta aatgccacaa cgacagtagt
     1801 accaagatcg atagcagcaa aaaaagccat aatgaccata ttgaagctgt ccgccaatcc
     1861 cacgccggcc atcgcatctt ttcccagcca gctgaccaga aaagtgctca ggacacccat
     1921 caacaggaca caggcattct ccatgaagat aggaacagca agcggggtta tctcgcgcca
     1981 gaacaacact ttgtagctct tgcgtttagc gtgccagcga gtgccgtgaa caacctggcg
     2041 taaagcagag gagatattca aagccgacct taattgcaga aagtgaaacc acatttcaaa
     2101 taatgaggga gaatcagcaa agctgcaaag attttcgcca acaaattgtc tgcaaatgca
     2161 acaaactgtt gatagaaacg gcaaacagtt gggaaattta aaaatcgggt ttgacaaaag
     2221 atttttcgcc gttaagatgt gcctcaacag cgattcctct gtagttcagt cggtagaacg
     2281 gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca aatttctgaa
     2341 aagcccgctt ttatagcggg ctttttgcta tatctgataa tcaatttcct cttcactgtt
     2401 ctccatcacc tggcgcttga tatcctcaac tgacagtcct gcattacaaa gttccagaaa
     2461 gcgccagaca tagttacgtt gaagttgccc tcgcttcagt cccaaccata cagtattggc
     2521 atcgaaaaga tgccgcgtat ccagacggat taaattctct tcctcttgtt cgccgctgga
     2581 ttgttcggca actaatccaa tccccagccc aagagcaaca taggttttaa tgacatcaga
     2641 atcctgcgca cttaatacaa tatctgccag caaacctttg cgggcaaatg cgtcatcaat
     2701 acgtgagcac cccgtaatcc cctatcggta agtgattaac ggccacttcg ctattgattc
     2761 cagcgtcaac ggtgtaattt gcgtcaaggg atgatcgagt ggaacaagca aactatggtg
     2821 ccaacgaaac cacgggaagg cgacgagatg cgggtcatta ctcaaacgct cgctggcgat
     2881 accaatatca gcttcgccat tttgcaacaa cgtcgcaatt tcctgtggcg taccctggat
     2941 tagctccagc cgaacctccg ggaaaagttc gcgaaaagct ttaatgacct caggcaagct
     3001 ataacgtgcc tgagtatgcg tcgttgcaat agtgagaacg ccagacgtat cgttggtaaa
     3061 caggtctgca agccgacgaa cattactggc ttcattcaga atacgttctg caatgaccag
     3121 taatgctttg cccggttcag tcatacccag cagtcgctta cctcgtcgaa caaatatttc
     3181 gatgccaagt tcatccccca gttcccgaat atgacggctg acgccggact gtgaggtaaa
     3241 aagcatattc gcaacctctg tcaggttgta atcctgacgt gcagcctcgc ggattatctt
     3301 tagttgttgg aaattcacgg taaactccgg gcagctcaga tttcccgtta ttgttaaagt
     3361 ctaatgcccg gcataacaaa taataaaaac ccgcatctta ttccatcccg atataacgct
     3421 tagctcacca attgccactg ccttttttcc atcactggag aactaatcac tgacattaac
     3481 aactctttca ctgcctgtgc ctgtggcgat aagttcgctc tggcgggtaa atttaatgac
     3541 aaagagagac tcatggaagg cgtggtaatg cgtgacatcc acccatttac tgcgccacat
     3601 aacgaacgcg cggccgattc gggtaatact gcaacgccca tgccgctggc aatcgctgcg
     3661 gtaagcgtgg caatagactc aatttcacca atgacttttg ccgtgagccg ccgtagggaa
     3721 aaagcctcat caacacgaag tctaacagca ctgtaatcac tggggagaaa gaggttcatt
     3781 tgcgcaatag cattaacatc aacgctttgc cccgggcaat cttgagttcc taccaggaaa
     3841 agatcttctt tcagcaaagc ctgactggat accccagcca caggggaatg ctcataaatc
     3901 accgccatat cgagttggtg atttatcagt ttttcgttaa gcactgcacc gctattttca
     3961 tgaagataga taacgatctc cgggaattca gcgcgaaccg cttgtaataa gggcatggtg
     4021 atagatgacg cagcggttcc tggcgcaaag ccaatcgaga cttgccccga taatgcctga
     4081 ccaacgttat gcaccgccag ttgggcctgc tcacactgac gtaaaatggc ccgcgcatgg
     4141 gtatagagaa tttttccggc gtctgttggc gtaacgcccc gttttgtgcg aatcaaaagt
     4201 tgttgattta actcaccttc cagtgtggca acctgctggc tgagcgctgg ttgtgcgata
     4261 tgcaatactt cagcagcctg agtcaggcta ccaatatcta caatttttac gaagtatttc
     4321 aggcgtctga agttcatgtt gcctccggtt tttgagaatc ggcccaagtg ccgccattac
     4381 ttacaaccag actgcaagat gcttgccagt tttattttgg cgttgatgta caagctaacc
     4441 aactgtcaaa taagagatta tgatagattc gtcattggct cttttcacca gcagtcgcgt
     4501 tcccctgccc tataaaagga gggtatgcac cacgatggtt cattaccaat aagattgaaa
     4561 tctcatcact ttgttgaaat tggcagcaaa caaacaaaaa aatgcatttc accctttgac
     4621 atcaccatgc actgccatta atatgcgccc cgttcacacg attcctctgt agttcagtcg
     4681 gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa
     4741 ttcaaaaaag cctgctttct agcaggattt ttgctttcta attaccaacg ctcataaaac
     4801 atctgtcttg aaccagaact aatttgcaca ggcattcccg atcgacgttg caacgcagca
     4861 tttgcgcgat tcacatcaac ttcttgcccg ttgataaacg cccgcaaaga tggggttacc
     4921 ggcaatggca cttttcggtc agactcatat tctgcacgat tgcgcgacag cggctcatga
     4981 acttccagcc agttcgagct atctggttca gtggtgtatt ttactggctg gtcgataatt
     5041 tgcacacgcg ttccaacagg aacattatca aacagatatt tgatatcgtc attgcgcaga
     5101 cgaatacagc cctgacttac ccgaagtcca ataccaaaat tggcattggt accgtggatg
     5161 gcatacaacc tgccaatata aatcgcgtac agccccatgg gattatcggg ccccgcagga
     5221 acaaatgcgg gcaaactctc ccctcgtttc gcatattcgc gccgagtgtt cggcgttggc
     5281 gtccaggttg gagcttcttg tttacgttca acggtagtca cccagttacg cggggtttct
     5341 cgcccagcct ggccgatacc aataggaaag acttccacag tattactgtc tggcgggtag
     5401 taataaagac gcatctcagc gacgttaacc acaatccctt tacgaacagt ggcgggcaaa
     5461 atcagttgct gcggaatggt gagttgcgag ccagacttcg gcaagaaaac atcagcgccc
     5521 ggattcgctt ccagcatgtt acttaaccct tgcccgtatt gtgcggcaaa agtctccagc
     5581 ggctgggtat tgtgatcagg aacagttaca gtaagcgact gccccaccaa acggctaccc
     5641 tctggaggta atggataagt taccgccagg ctagtatggc tggcaaaaag cagagcaaat
     5701 gagcaaagaa tatttacacg acgcatcatg tccctttcct atgtcgcgaa agctatccgt
     5761 taagtatagc ttttatcaga cttttcgttt ttaactgttc aaatcagaag tcgtattccc
     5821 cggtagaaca atattactgg cagcaagttc gcccatgttg ttatatatcg cacaggcagc
     5881 ttcgatgatg ggcatcgcca gagctgcgcc actcccctca cctaaacgca tctccatatt
     5941 gagataaggc tccagcccca aatgcgacag cgctatacgc gccccttttt ctgccgacag
     6001 gtgagaagga atgagatacg gtttgattgc aggagacatc tggcaggctg cgagcgcagc
     6061 agcataagaa agaaatccat ccagcaacac gggtaaaccg caggaagcag cgcctaacat
     6121 cacgccagcc attccgacca aatcaaatcc ccccactttt gccagaacat caacgccatc
     6181 ctgcggattt ggttgattca acgtaatcgc ccggcgcaca acatcaattt tattagccag
     6241 tttatctgtc ggcaggtttg cgccaatccc aaccacttct tcaggatccc ggccagtgat
     6301 tgtgctgact attgccgctg ccggtgtcgt gtttgccatc cccagttcac ctacaccaaa
     6361 cagcgtgaca ccgttttttg ccagctcccg cgtataacat atgacgtcca aaagcaactt
     6421 ttcagcctga cggcgactca ttgccggagc tgaagcaata ttgccgctac ctcgtgcgac
     6481 acgcatgttg ataagcccgg ggataggctc agcactatca ataccaacat caactacgtg
     6541 gacgttagcg cccgcttgtg ctgccagcac acacacgccg gttgttccgc gggtcatatt
     6601 ttcagcctga atggctgtca cttcttttgg ggaaatagcg accccttcct cccagacgcc
     6661 gtgagcggca cacataacca gtaccgcttt tttgcccaca tgcggtatgc cattcaaccc
     6721 cggcattccc gccagttgta tggcaagcgc ctccagcctt cccaggctac caacaggttt
     6781 gagtaacccg tcaatatgcc gttgtgcacg cgacatagcg gcaggatcga tagcagggat
     6841 cgtattcagt aaatcggcaa gtgtttgcat gtcacgtctc tttatagcag tgccagcagg
     6901 aagaccagtt caccaagctc tatagcagct cccagcgtat cgccggtttg cccgcccagc
     6961 gtacgtttga gaagctgacc gaggatgaaa atcgccacca tcgtcaccac catagcagcc
     7021 acaccatgca tacccggcaa taatacggca gcaaaaatag cggctaagcc gagtgtgacg
     7081 caggtttgtc gcccatcaat tttgccaatg aacacattgc caagcccttc ttcccgggca
     7141 taacgatgac gatacatcaa taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc
     7201 agcgatgcaa ggatcggttc gccacgcaga gccaactcgc ttaataccag aatctttgcc
     7261 agtacaacaa aaatcaatgc cagaccgcca tgggtgccta aacggctatc acgcatgatt
     7321 tccaacatgc gatcgcggct acgtgcagaa aatacgccat cacaggtatc ggcgaggccg
     7381 tctaggtgga atcccccggt catcagcgcc agcacaagta cgctaaacag tgccgccagt
     7441 ggtacgccac accatgcctg cagcaccatg aagaccagcc cgctaatcgc gccaagcaat
     7501 aatccaatta aaggaaaagt aataataccg cgagaataat gctcgaaatc cagtccctgg
     7561 gacctgcgac gcggtacagg cagacgcgta ataaatgaga gcatcgccca aaataattta
     7621 ctcatttgat ttttactcca atacccgaaa ccaccagcca tacttcattt gccgcagcgg
     7681 ccaactgctg atttacccgc ccggcaatat cacgaaaatg tcgtgccaga cgactctccg
     7741 gcacaatccc cattcccact tcgttagtca ctaatacaac ctttgcgggg caacgttggc
     7801 aggcagcaat caacgactga atctcagcat taatcgcctg ttccatcgcc tgataatccc
     7861 attcatcagg gtctttatcg ccgccataat caaacaacag attagtcacc attgtggtaa
     7921 cgcattcaag caacacaacc tcattcgggt taatgtctgc atgaattaat tcatcaagat
     7981 gttgccagcg ctccactgtg cgccagtgct ccgggcggcc ttgccgatga tgttctatcc
     8041 gtgcagccat ctcatcatca aggatttgcg aggtagcgat atacagaacc tgtgaagagt
     8101 ccccaataag cgcctctgcg tggcgactct tcccgctccg tgcgcccccc gtcaccaaaa
     8161 tcatcatacc gactcctgat tcaaatgtat gatggtattc attgaacaga acaatggatc
     8221 ccccaccgtt gctgaccgca aaacatttac gcgattaata ctgcgcgtaa tataattaaa
     8281 aatccagcat tctcaatcca ttacgaaata agatggcatt acggaatagc cagactttct
     8341 cgtgttaacg tccgacaaaa tgcgtcattg acagcggttt ctgtggattg acacatttca
     8401 ttcagataat gaattaatgc tactgccgga acaaccagca aacgggcatt gtgttctgaa
     8461 atccaggcta ttgattcaac gtcagcgaca gatctgcgct gtaaaagaac ttgtaaaaca
     8521 acgtaataga attatccgga atggtggcga ctatgcacta gaacaccaca atttcgctct
     8581 ctcggtaagg cactgtcaca gcattcaaca gaatgtgact tgccagattt attagcgcca
     8641 ccagatgttt aaccgggtaa ccacgaccca gtcggggata tattccatct tctcgctgac
     8701 atcatcaaga ataaaaaggt ttatcacact cagagcaacg ttcttcagca ggcgggataa
     8761 ttcttcaaca tttagaagat gtgttattaa aggcatacaa actggacgta ttgtattttc
     8821 ttttggtggt tgacctaagg tagcagttta tcctgatgcg ctgagatttc tgtaatatct
     8881 acgtcaacat tctcctcgaa tagaaaatgc agccccggca agtgattcac attttttaac
     8941 aaaacgttgt tagcgtgcca ttttcagaag ctctccaaga aagcgaattc ttttctcaaa
     9001 ttctgcattc tcagacacag ctttctactc catggtaatg gcccaaatac gaagttgctc
     9061 aggatcatta gtggtgctga gaagttaaat aaccatgcgg agtaccactc gtcatcacgt
     9121 atttatatat catgaagcta ttgttgtgtt ttgtacatca gtagatatat tctgatatac
     9181 tccttttgct agacataacc tttcacctgc ttgcaaagct tctgtgttct gacattgcca
     9241 aattgttgca attctgtatc cagccttctt tcagtcatag cttcgggccg cgataagact
     9301 cactgatctg accctgattc ctcttgcaga ctttatagac caattaaaat gcagtttctg
     9361 caggtcaacg tctgaccatc attgtcatca ctctggccat tagagtaacc ttctgcattc
     9421 atccttttgt aaaaagttta tgtcagtatc agcaattaac cggacctgat actgatatga
     9481 gtcttaccgc atatacggtc aatttcagca attaattaca ttatccacgc caaagtgttt
     9541 gtcatcgcaa tgatggtacc ttctttcaga caccattttt tcaactccgt tttccacgga
     9601 ccgcactctt atgtcaagag tgcggtgatc ctacccacgt aatatggaca caggcctaag
     9661 cgaggttctg gttttcaaat tgttccggac tgaggccgcc acaccaactg tgccgccgcc
     9721 accgattgta atcacattcg atataattaa acaccgttgc ccgcattatt tcccggctga
     9781 taaagtgttc tccatggata cattccactt tcagcgaatg aaagaagctt tccacgcaga
     9841 ttatgccgct tcagttgcgc ctgataatct gctgaacagt actggcctcc acggtccgtg
     9901 tgaacgataa cgttccgggg cctcttacgc cgcca
//
LOCUS       DAZFWL020000159         9914 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFWL020000159 DAZFWL020000000
VERSION     DAZFWL020000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.159"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(34..207)
                     /locus_tag="ACHKNP_RS21320"
                     /old_locus_tag="pgaptmp_004264"
     CDS             complement(34..207)
                     /locus_tag="ACHKNP_RS21320"
                     /old_locus_tag="pgaptmp_004264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706319.1"
                     /translation="MNKNAWVYFIDAIGEATKLDVLVNGKKVDSYTANIKNEKKTLGN
                     KFYGSCRNTWFQE"
     gene            complement(623..1693)
                     /locus_tag="ACHKNP_RS21325"
                     /old_locus_tag="pgaptmp_004265"
     CDS             complement(623..1693)
                     /locus_tag="ACHKNP_RS21325"
                     /old_locus_tag="pgaptmp_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HGU4706320.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGSQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     HAGALLTTLFRHISIINGFDNPMTQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKHAGDGGNSGGLLSFTSAHIDAWRQQGYEDTMLTMEHIRKPLAARQALTRSEAVL
                     QKSQEITEGADSALRNAMAQIK"
     gene            complement(1690..2595)
                     /locus_tag="ACHKNP_RS21330"
                     /old_locus_tag="pgaptmp_004266"
     CDS             complement(1690..2595)
                     /locus_tag="ACHKNP_RS21330"
                     /old_locus_tag="pgaptmp_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HGU4706321.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVVLEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            complement(2592..4976)
                     /locus_tag="ACHKNP_RS21335"
                     /old_locus_tag="pgaptmp_004267"
     CDS             complement(2592..4976)
                     /locus_tag="ACHKNP_RS21335"
                     /old_locus_tag="pgaptmp_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HGU4706322.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHTQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGSDFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFVREAFGRMKKDYVCKDSELATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRRAALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATEKIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVQTNLSALMEERTETVTRRRRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVINMNKVRKEVMSLTRAYFGELQASIEQNINQPVRQEIDDFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQEHLTERLQALNERVPELITDSKALREELETML"
     gene            complement(5194..6038)
                     /locus_tag="ACHKNP_RS21340"
                     /old_locus_tag="pgaptmp_004268"
                     /pseudo
     CDS             complement(5194..6038)
                     /locus_tag="ACHKNP_RS21340"
                     /old_locus_tag="pgaptmp_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
     gene            6362..6949
                     /locus_tag="ACHKNP_RS21345"
                     /old_locus_tag="pgaptmp_004269"
     CDS             6362..6949
                     /locus_tag="ACHKNP_RS21345"
                     /old_locus_tag="pgaptmp_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706323.1"
                     /translation="MGLDDFGLSILAGLLANRLDFSKPKGTSAQMNEVIPDVIEEPDK
                     FSLSANLPKRFKTFDVWSDLDELLKVVKEPKISVLIEEQPSTYYRLPSLVLESQITGE
                     WYVFPRGRMSFEGNGGGIQNAEVIFNQLKEENVPVGVWVIDQALLDRLDNGQECWPTI
                     RLQIVPFLAAPSSEYPWFEIQRNVAKLTEFHLTND"
     gene            7281..7907
                     /locus_tag="ACHKNP_RS21350"
                     /old_locus_tag="pgaptmp_004270"
     CDS             7281..7907
                     /locus_tag="ACHKNP_RS21350"
                     /old_locus_tag="pgaptmp_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706324.1"
                     /translation="MKKIIYTFSLVVALLLALRCAAYIDFSEKDMLKSIIPAWIQAIG
                     SILAIIIAGMISANQIKHEKQLEKRKVAESDLAKMHIVRALIIRSLTLMNEVRQAIER
                     GEESDFQQVSPWTMRNTKRAIDNLNLFEIPEGMLALDLLAIGPSLEEIATLWERCMEE
                     SLHTENNKPTIDSYNELDQGSQEVLKICEAAFKLATQEVNRLAAIVKE"
     gene            8364..8789
                     /locus_tag="ACHKNP_RS21355"
                     /old_locus_tag="pgaptmp_004271"
     CDS             8364..8789
                     /locus_tag="ACHKNP_RS21355"
                     /old_locus_tag="pgaptmp_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4706325.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIATSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            8786..9136
                     /gene="tnpB"
                     /locus_tag="ACHKNP_RS21360"
                     /old_locus_tag="pgaptmp_004272"
     CDS             8786..9136
                     /gene="tnpB"
                     /locus_tag="ACHKNP_RS21360"
                     /old_locus_tag="pgaptmp_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4706326.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRRNSLTML"
     gene            9167..>9914
                     /locus_tag="ACHKNP_RS21365"
                     /old_locus_tag="pgaptmp_004273"
     CDS             9167..>9914
                     /locus_tag="ACHKNP_RS21365"
                     /old_locus_tag="pgaptmp_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HGU4706327.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMT"
BASE COUNT         2464 a         2512 c         2377 g         2561 t
ORIGIN      
        1 attaaactga ggtgttttac accacgttat caattattcc tggaaccagg tgttcctgca
       61 gctaccgtag aatttgttac ccaaagtctt tttctcattt ttgatatttg cggtatagct
      121 gtcgactttc ttaccgttta ctaaaacgtc gagcttcgtg gcttcaccga tagcatcgat
      181 aaagtatacc cacgcattct tattcattcg tgttccgtcg acaggtgggg ccgaatactt
      241 tttaccgttg atgataaaaa taatatcaga atcgtactga tttagcgggg tgggggtatg
      301 accgtaaatc cactcatatg tggtagcaaa ttgttgtctt ctggattaat cgtgcagtta
      361 atagaaacac taccgatact gaacatatca gtgccagcaa cactgttacg cttctacaat
      421 gccgattctg cttgtgctac ggttgtactt acagttaaaa caacagctaa aagaatgttt
      481 aacagtttca tgagatttct ccccaaaatt aaataaatta atcttaacca tcatccaggt
      541 aatatatatc tgaaaatatt ttcaataaca ccagataaga aaaacaccac cacagtggcg
      601 gtggcgtttc tccggtgtgt ctttatttaa tctgggccat cgcgtttctc agtgccgaat
      661 ctgcaccttc agttatctcc tggcttttct gtaataccgc ctctgacctg gtcagtgcct
      721 gacgcgccgc cagtggtttc cggatatgct ccatcgtcag catcgtgtcc tcgtagccct
      781 gctgacgcca ggcgtcgata tgtgccgacg tgaaactgag cagaccgcca ctgttgccac
      841 catcacctgc atgtttcagc cttttccggg gacggatctc aaggattgtg gtgtccggaa
      901 aagcctggcg atcccataaa gaaccgtcac tcagatgcgt cacaatcacc atattgcatc
      961 cggcatcgac cagaggcgtt acaggggtat ttccctgcat atttcgccat cctcccatac
     1021 caccatcacc gaacattgtc ccctgaacct cacggggacg gaacaacagg ggcagcgcgg
     1081 ctgacgcaag cagagcctct ttctgctgtc cgcggggcag actctggata tgctgaaaaa
     1141 cggcgttttt cgtggttccg acacccagtt cagcacgaat acagtcaata atatcctgca
     1201 tgcccccttc tgtggggtac agcgacacat acagcggtag cccgtctgcc agagcatcag
     1261 tatcaagata atgatccatc agcgctgtca ggggctcatc tgacaacaac ggttgagtca
     1321 tcggattgtc gaacccgttg attatcgata tatggcgaaa aagcgtcgta agcagtgcgc
     1381 ccgcatgtcc ggggatctgg cagaggttca ttgcctggaa caattttttc agcaatgaaa
     1441 agtaaaccga tctgtttacc gacagcacct gattattccc cagatgatcc cagagcgcct
     1501 ccaggcgtac ggcagcttct gacagatcgg gagaggccgc gataatggca ccattgaatg
     1561 cgccaatgct ggcccctgaa accatgctga tctgtgaacc acactctgcc agtgccttaa
     1621 ccactcccgc ctgataagcg cccaccgccc cgccgccgga cagcaccagc cccgttttca
     1681 tttctgtact catgcagccc ccttctgaaa tatctcatcg accatacttt cagagaggcg
     1741 ttctgcagag cagagacggg gcatgcgaac tggtaagtgt tctgcagcct gcgtggtcga
     1801 ccagatcacc ggatgccgct cttcactgca ccagtccccc agccagttaa tcgcgtcatg
     1861 ccactcgtta tctgcctgct cccggccctg accaccaagc cagtccagag tgttcagacg
     1921 cacggttgca ctaccgccac tctcctgtgc catctgcgtt aatgctttat gtctgagaat
     1981 atccagtaac tggttaacct ggcctgtttc accctgacgt atcacatcac caaacgcgcc
     2041 ccagcgaagc tcttcaagca ccacataaca gcgtccggca ggggaaaatg acgcgtatcg
     2101 cccggcgctc caccatgaga atatctgctc aagatgtaac tgtgcccgct gaacctggtc
     2161 aacacggtgg agtttttctt caagatgatt cagtttctgg tgaacctgat cggccagggt
     2221 gagtaactgc tcccgcgtgt ctgccgaata atgcgccagc ctggcagtat cactgaccag
     2281 tgaactgacc ctgtcgttta cccgggtaat tgcgtagttt gtggtggcaa gtgacgttgt
     2341 catctccgtc agccagcgca gggacgcctc aacgccctgt gcaagcgaag cattgatggc
     2401 attctgccgt gcagcgcctt cgccggtcag cccctctttt aaacgccgga agaacgtgcg
     2461 ctctccggcc gtgcggatat gctcctgtgc aacatcgata ccgtttgcaa aatcaaccac
     2521 aaatttttca ggaaggcagg tgagtacctg acatacccgg ttatcagcaa tttgttgaat
     2581 aaatggtgaa gtcacagcat tgtctccagc tcttccctta acgccttact gtcagtaatc
     2641 agctcgggaa ccctttcatt taatgcctga aggcgctccg taagatgctc ctgtgcctgc
     2701 tgatcgcgtt tatgatcttc tgagctctga atgagtgtgt tacgtagctg ttcgactttt
     2761 tccctgaatg tacagaaaaa atcatcgatc tcctggcgga cgggctggtt aatattctgc
     2821 tcaatggatg cctgcaactc cccgaaatat gcccgggtca gtgacatcac ttccttcctg
     2881 accttgttca tattgatcac gctgcggctc acatccacac tgtactcttt ccagccccag
     2941 tcactggtgt ttgcccatcg acaaagcgta ccccagacac catcctgtcg acggcgacgg
     3001 gttactgtct ccgttctttc ctccataagc gcgctcagat ttgtttgtac cgcgagccgg
     3061 gtctgtaact ggctgactgc cggaaaactg atttttaccg tgaagccctc atcctccagt
     3121 cgggcattga tgcgtccggc gatcttttct gtggcataca gggccgtacc acgaaaacct
     3181 gtttcaatcc ccctgacgat tttttccagt tcgggccgaa actgtgcctc agcctcacgg
     3241 tgcaggctgg tcacggtcga ttcgatttgc gtcatcagtt caagggctgc cctgcgatca
     3301 ctgaatttta tttcggggct gtccggatca aagtcccgca tcctgtcatg ctgatttccg
     3361 gttccgaaaa tatcgtgaaa gaaagcgaat gcgtttctct cccggggctg cgctgaattt
     3421 tcttcttcca gcatttgctg ttctttacgt ttaccgcttc tgaaatagtc gttcagctcc
     3481 tcctccagtg aggcacagac cttcgtcagt aattccccgg ttttggtatt gatgtcctcc
     3541 atggccattc tgacatcacc ggtcacctga ttctgacatt cctcaatctc ctggatatcc
     3601 gccagtaggc tggtaatatg cgcctgcagt gactgaatgc tttgctgcag cccctgatgg
     3661 cgtaatgaca gatattcgct gacattttcc gcattctgca tcagttttgc ggcggcagaa
     3721 tcaaccgcca gtgccgccgc tctggaatgg gagctctgta tgacttccgt tatcagttga
     3781 tcgataagtg agccttccca caggtctgtc gccccttcag ttgccagttc gctgtcttta
     3841 cagacgtaat cttttttcat gcgaccgaag gcttccctga caaaatcatc gacccaggct
     3901 tcatcaacag gaagggttcc gtttttccgt aactcatgaa gcgcacggtt agccaggtac
     3961 gcctgacgtg aggatccggg ataaacgcgg gaggcgggca gcacatcgct gttcagcatt
     4021 gccggtacct tctggcgtac ggcatctgct ccatcgccat tgcggtcttt ctcatcaaat
     4081 ttattgacca gcacaaacag gcggccggct gatacgtcag caatggcatt aagctcttta
     4141 cggacgtctt catccgcttt tgagttcatc tgggtgtaat ccatcactgc cagcaccgcg
     4201 gaagctttct gcagttgatc ccgcatcatc acttccatct gtggctggcc ggcttcatta
     4261 gggccggggg tatccagcag tgtgagtgtt ccctggcagg catccatccc caccagatgg
     4321 ctgaattcca cgtcgatcac cggcagtttc tgcacttctg cgtattcatc aaaaggaaag
     4381 tcactcccca ttgccgcagc aagacgaacc agatcgttta acgatgccag tccggtaaag
     4441 atttcctcct ccccgtggta ttcatttttg agccagccat catccgtcag aatatccagt
     4501 agttcgcggg tatccccggt ttgctgcagg gtctgtgcga cctgctgtcc tgccggggtg
     4561 gcgagttttt cctgcagtgt gattaataaa ttgcggacag gctgagtatg ttccagatgg
     4621 aggaccggct cagtttttcc aggaacgtgt cggatgagtg tcggaacaga ggtcatgggg
     4681 cggttacggt tgggcagaat ttcctgcccc acaatggcgt tgatggtggt tgatttacct
     4741 gctttcatcg tgccgacaac agccagcacc atctccctgc gggcagtttt gatttgttcg
     4801 ccctccagct cctcaatcct tttcagtgct ttctgtttat caaaaagcag tccgttttcg
     4861 ttcttactgt cagataacac atccggctca tcaaccattt gccggagcag attgatgttc
     4921 agttgcaaaa gtcggtcggc ttcatcacaa agcagggcga tgttcttttc gtgcattatt
     4981 taaccttcct gagacgcatt ttcatgacaa ccacggagga ggccatttat tacaaacgat
     5041 cgtttataac gatcgatgtg atagtaacga tcgtttaatt ctattaactt aaaaaacact
     5101 acgcaagggg acaacccggg tatggctgcg tttgcatagt gttttcatgg agttaaaaat
     5161 ctgtaacaac agcctggtgc atcaccacca ggttcagaag aaaatccggt tccataccgc
     5221 gcgggcaaca gagaccacgg tgtcacgaac agcgcgcagg gccgtacgga caacagaggc
     5281 aacacagacg ctctccgctg tgtcaaatat ccggtccacc gcaccgataa actgttcacg
     5341 ggcctgagag cggacagact ccgtgcgcag ctcgtcctgc agtcgggtca tcaggggact
     5401 ggcggcatgg tcgggaagcg ctgtcatgag cgcactgacc agcgtatccg gctcccggcc
     5461 ggtgcgggcc gaaacagccg gaataccgcc tccgtttttt cgcgaatgtt ctgtgcctgt
     5521 gcgggagaag gctgaatgcc ggccatatcc cattcatggc agggttccgt tttgtcagcc
     5581 tgcgtcacca caaacagcac ctgctgatgt ccccggtgca ggatgtgtcg ccagaaatac
     5641 tcatccacag acagggcccg gtcatcggct ttaatcagcc acagcaccag gtccagttca
     5701 ggcagaatgt cacggtacag ggcttcagac tctgcatccc tgtcccggct ctcgcccacc
     5761 ccgggcaggt cagtgataac catgctgtga ccgtgaccac tcagacggaa gcgccgtact
     5821 tcccgggtgc cggcgtgaac atcactgacc ggggtgacca ccccctgaaa cagtgcatta
     5881 cagagtgagg atttaccggc cccgctttta cccataatgc caatcacggg ttcgtgactg
     5941 gtgagtttgc gcagatgttc caggatgtga cgggaaagcg agtaaggcag ggaggagagc
     6001 ggtttttcaa ttgcctcaat ggcatcagac ggattcatac atgtattccc ggtgaaaaaa
     6061 aacaaaaccc cgcgcaccgg aaaggtggcg ggggattcac ggagataata tctgtctgaa
     6121 attatttagg atgcaggtat atgcagttga tttgtatcta gtacagtact aaacctaatc
     6181 tggctgtccg ctctgtgcca ggagcagacg tctaatatat ctacttggtt cttaaattca
     6241 gtgcatcact gcaagcagct caacatcaat aaattaggga ataactcaca actttgactg
     6301 ttaccacatg gcatcatagt gtttagagca tgcataagca ccaacgagga gttttaacat
     6361 aatgggactg gatgatttcg gacttagtat cttagccgga cttcttgcaa acagacttga
     6421 tttctcaaaa ccgaagggca cctcagctca aatgaatgaa gttataccgg atgtgattga
     6481 agaaccagac aaattctcgc taagtgcaaa tttgccaaaa agattcaaga cattcgacgt
     6541 atggagtgat ttggacgaat tgctaaaggt tgtgaaagag cccaaaatat ctgttttgat
     6601 agaggagcaa ccatcaacgt actatcggct ccctagcttg gttctggaat ctcaaatcac
     6661 aggggaatgg tatgttttcc cgcgaggccg catgagtttt gagggcaatg gaggtggcat
     6721 acagaacgca gaagtaatct tcaatcagtt gaaagaagaa aatgttccgg tgggagtatg
     6781 ggtaatagat caagctcttc tcgaccgcct cgataacgga caagaatgtt ggccaaccat
     6841 acgattacaa atagtaccat tcttggccgc accatcgagc gaatacccat ggttcgaaat
     6901 acaacgaaac gttgcaaaac tcacagaatt ccatttgact aatgattaat agtgagccct
     6961 aacaggataa tggcacactg gatatgacca cctaagcaga ggcagcacag gggtaataaa
     7021 ctatgaactt ctgcttttcg ctcacagcgg accttcagct tcgttaacac gtccgctccg
     7081 tgccagtagc agactagtga tcgctaggct tgaattcatt cgtattagcg ccaagtggat
     7141 aataaatggt gaaatccatt agtcattact ttatcataag ctcgttatat aaagatttta
     7201 gtattcgtaa ttcttttctt tgtttaagat gatgaaaatc atataatatg atgtgaagaa
     7261 gtatagaggg attaatcatc atgaaaaaaa taatatatac tttttctttg gttgtggctt
     7321 tattattggc gttaagatgt gccgcctata ttgatttttc tgaaaaagac atgctcaagt
     7381 caatcattcc tgcttggatt caagccattg gtagcatttt agcaattatt attgccggta
     7441 tgatctcggc caatcaaatt aaacatgaga aacagcttga aaagagaaag gtagcagagt
     7501 cagatttggc taaaatgcat atagtaagag cgctgataat acgttcgtta actttaatga
     7561 atgaggttcg tcaggcaatt gagcgtggcg aagaaagcga ctttcagcaa gtgtctcctt
     7621 ggactatgcg taatacaaaa cgtgcaattg ataatttaaa tttatttgaa ataccagagg
     7681 ggatgttagc tctcgatctg ttggctatag gtccatctct cgaggaaata gcaaccttat
     7741 gggagcggtg tatggaggag tcactacata ctgaaaataa caagccaact attgattctt
     7801 acaatgagct tgatcaaggc tctcaagagg ttttgaaaat atgtgaagcc gcttttaaat
     7861 tggctacaca agaggttaat cggcttgcag ctatcgttaa agagtaactt tcacatagga
     7921 atttatttta tatgtgctct tagagtacat tacttttagt aatttgtact ctttggttgt
     7981 atatgccgac aacgatatag gttttaaagt attactgtga atttatgggg taacatcatt
     8041 ataagccgat taatcctcct agattactta cctatccttg aagaactgtc ctacagcccg
     8101 cttcgtgtca taagcgggct gtaggcatag aataactaat caatcattag agtgagacta
     8161 ttttcagctt tggtgaggtg ggatgatgat tgctttagag tttaatagga cactccggtc
     8221 gctcccttcg tatctggatg taaagccccc tattttttct ccaaaacctt accttatcgt
     8281 aagcgtaaac tgaccgccgt atgtagccat cagacgagaa ttggtaactt agacgcccat
     8341 ctgatataga cggacatcta agtatggaat tacaggactg gcgaaaagaa cctcgtaaaa
     8401 actattcgaa tgaattcaaa cttcgtatgg tggaactggc atcacaacct ggagcttgtg
     8461 ttgcacagat tgcacgtgaa aatggcgtca atgataatgt tattttcaaa tggctcaggc
     8521 tctggcagaa cgaagggcgt gtttcgcggc gtcttccggt aacgacctct tctgacactg
     8581 gcgttgaatt attacctgta gaaataacgc cggatgagca gaaagaacct gtggcggcca
     8641 ttgcgacgtc tttatccact tccactcaga ccagagtcag tgccagttcc tgcaaggtgg
     8701 aattccgtca cggtaacatg acgctggaaa atccatcgcc agagctgctc acggtgttga
     8761 tccgtgaact gaccgggagg ggaagatgat ctcactccca tcaggtaccc gtatctggct
     8821 cgttgccggc gttaccgata tgcgtaaatc cttcaacgga ctgggagaac aggtacaaca
     8881 tgtgctgaat gataatccct tctccggtca cctgtttatc ttccgtggcc gacggggtga
     8941 caccgttaaa attctttggg ctgatgctga tggtctgtgc ctgttcacca aacgcctgga
     9001 ggaaggccag tttatctggc ctgcggtacg tgacggcaag gtatccatta cccgctcgca
     9061 actggcaatg ctcctcgata agctggactg gcgtcagcca aaaacatccc gccgtaactc
     9121 actgacaatg ttgtaaaaaa ctcctgaccg cattataaaa acggtcatga gtcagaaata
     9181 cctcattcgc atcgcagagc tggaaaggtt gctctctgag caggctgaag ccctccgtca
     9241 gaaagaccag caactgagtc tggttgaaga gacggaagcc ttcctgcgct ctgcactgac
     9301 acgtgccgaa gaaaagatcg aagaagatga acgggaaata gaacatctgc gggctcagat
     9361 agaaaaactg cgccggatgc tgttcggtac ccgttctgaa aaactgcgtc gtgaagttga
     9421 actggctgag gccctgctga aacaacgtga acaggacagc gatcgttaca gtgggcggga
     9481 agacgatcct caggttcccc gccagttgcg acagtcgcgc catcgtcgtc cgttaccggc
     9541 acaccttccc cgtgaaatac accgcctgga gccagaagaa agctgttgcc cggagtgtgg
     9601 cggtgagctg gattatctgg gggaagtcag cgctgaacag ctggaactgg tgagcagtgc
     9661 cctgaaagtg atccgcacag aacgggtaaa aaaagcctgt acaaaatgtg actgtattgt
     9721 tgaagcaccg gcgccgtccc gcccgataga gcgtggtatc gcgggccccg gattacttgc
     9781 ccgcgtgtta acgggaaaat actgcgaaca tctgccactg tatcgtcaga gtgaaatctt
     9841 tgcccgccag ggcatcgaac tgagccgggc attactctcc aactgggttg acgcatgttg
     9901 tcagttaatg actc
//
LOCUS       DAZFWL020000160         9907 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFWL020000160 DAZFWL020000000
VERSION     DAZFWL020000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.160"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..573)
                     /locus_tag="ACHKNP_RS21370"
                     /old_locus_tag="pgaptmp_004274"
     CDS             complement(<1..573)
                     /locus_tag="ACHKNP_RS21370"
                     /old_locus_tag="pgaptmp_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HGU4706328.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKLYIKISLI"
     gene            complement(570..2258)
                     /gene="yfaA"
                     /locus_tag="ACHKNP_RS21375"
                     /old_locus_tag="pgaptmp_004275"
     CDS             complement(570..2258)
                     /gene="yfaA"
                     /locus_tag="ACHKNP_RS21375"
                     /old_locus_tag="pgaptmp_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HGU4706329.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(2407..5034)
                     /gene="gyrA"
                     /locus_tag="ACHKNP_RS21380"
                     /old_locus_tag="pgaptmp_004276"
     CDS             complement(2407..5034)
                     /gene="gyrA"
                     /locus_tag="ACHKNP_RS21380"
                     /old_locus_tag="pgaptmp_004276"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HGU4706330.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            5181..5903
                     /gene="ubiG"
                     /locus_tag="ACHKNP_RS21385"
                     /old_locus_tag="pgaptmp_004277"
     CDS             5181..5903
                     /gene="ubiG"
                     /locus_tag="ACHKNP_RS21385"
                     /old_locus_tag="pgaptmp_004277"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HGU4706331.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(6032..9766)
                     /gene="yfaL"
                     /locus_tag="ACHKNP_RS21390"
                     /old_locus_tag="pgaptmp_004278"
     CDS             complement(6032..9766)
                     /gene="yfaL"
                     /locus_tag="ACHKNP_RS21390"
                     /old_locus_tag="pgaptmp_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HGU4706332.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGVVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGMLEVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         2313 a         2740 c         2386 g         2468 t
ORIGIN      
        1 aattaatgaa attttaatat acaacttcca tgtcataagt tgttgcagac tgactgcgcg
       61 cattccgttg tcagttttcg tggcgcttcc ggtgtggtag atgacgtaac gccccatcca
      121 gaccattaag tgctgggcat cgccctgatc gaaaaaaatc atatcgccag gcagcgcctg
      181 gtttatgtcc tggccaataa actggctgtt gtactgaatc aaattaatcg cggtcacgta
      241 ggggccggtt ttcccgttcc cctgattcca gttttgcgcc agttgacgct gtccaggtgt
      301 tagcgtcatc tctggcggca aatactggct agagaaaccg ttacttttta gccatttact
      361 atcgtgaact ttcagcgtct cgttcgccgc aaatcgcacc aggcccgcac aatcctgctg
      421 ataccagcgt ggacttggcc cctggcggag ctgttcttgt gcaatgcgta caaaccaggc
      481 gcgaaacagc ccggattgtt cgacattcag catttcgcta tgggcaacaa cacaacacag
      541 ccagcaaatc agcgccagca gcccgtgcct catagcggct gccaggttat gggtagccac
      601 tgccaggcgg caccgggttc catctgggcc agcttcatga cataacgcgg ttgttgagat
      661 aaagcgtcca gcttcggcat taataaagtt tgtgcggcgt tataaaaaac cggttcgaga
      721 ttcttcggca gactggtcaa cgtttcgtta cgcagcagtt tcgccacgcc ttgtggattg
      781 atatagagcg gaacgatgcc atcagtgggt attacatcca ccattgccgg gcggttttta
      841 ttcagtgttt gcagggcgtt attaaccagc gtgtcatcga gggagaaaag cagcgttttg
      901 ttttgcatcg ccagcgacac gcggaaaaaa taatccgaca ttaattgatc gggttgcgcc
      961 gcctgcgctt tcggatactg accgtatcgg gaactcactt cgcgacgcca gatttgcgct
     1021 tcgccctgct gagtctggct tatcggcaaa acaccttctg gcgctttgct ttcgtgcgca
     1081 ccaatatttt gcgtaaacag tttccctggc aattgcgcct gttcggcagt gccatcaaac
     1141 tgaccgacaa acagcggggt ttgcaatttt gagtctgcat accagcacag ccccgcggca
     1201 ccgtctaacg ccccattcaa cttgtcgttt tcctgactga tgtgcgaaag catctcttcg
     1261 gcaataccgt gtgaatacgg caccaccaca cagaagctgg ccccggcagg catactgttc
     1321 cagaccggcg taaaatcgaa actggtatct acgctggcgg attcatcatt taacgccaca
     1381 aaactgtgcc agccgtcgtt acccatttcg aagcgtacgc cagcaaaaga aggcattaac
     1441 cgctggtagc caaaacccag caacctggcg ctgaccacga tgcgctggcg tactggcgtt
     1501 ttttcagcag tacgctcttc caggccaaag cttgcttgcc agcgtttttt gccgctcaac
     1561 aaatcacctg cgatcgccgt ggcttcggta tcctgctgat catctttaaa caacatatcc
     1621 gtgctggaaa acaccagcat cttgtcctga taagtcgcga acatcagggc gttattgccg
     1681 ttatagcgca actgataaac aggaacagtt tcactattta tcttgatgct actgatttcc
     1741 gttttgctta actggctgtc gctggtagcg gcaaacaaca atggctccag cagtttgctt
     1801 aaaccactgc gctggatcag caccatataa tgtgaaagat ggccttgttt atcgtgccac
     1861 agcgctgcct gcgcgggctg atctaagagt gacgaaaaga gcttatcttt cagcgtgaga
     1921 tcgtgttcat agacaatacg acgaatgctg ccttcaatgc ccagacgatc ggcatgattc
     1981 tgataataga aaacgaaatc ttcgctcaga acatcgtgga gaaacggaat ggtgaggaga
     2041 tctttgggaa gctggctcag agagtcgctg tcgagaaaga ggtccggctc attgagatcg
     2101 atttgcagat tgttgtgcac caccagcggc gacaacgttt tttctggccc actgccagca
     2161 tattgcaacg cccagacgcc agcggaaagc agtgctattg cgccaaaacc tacaagacca
     2221 tagaaccgcc agcctttcgc ctttttttca ccactcattg ccacattcct tgtgtatagc
     2281 cagccatttt ttacgggcac agccaaactt taccgtgccc taatacgaca aaagcccaga
     2341 ctttgcagcc tggacttttc aattcaaaca agggagatag ctcccttttg gcatgaagaa
     2401 gtaaaattat tcttcttctg gctcgtcgtc aacgtccact tccggagcga tttcatcgtc
     2461 cccttccgcg gcactgccat cgatggtatc cagatcttcc tcgtcaaccg gttcagcaac
     2521 acgttgcaga cccactacgt tttcatcttc cgcagtacgg atgaggatca cgccctgggt
     2581 gttacggcct acgatgctga tttccgaaac gcgagtacgt accagcgtac cggcatcggt
     2641 gatcatcatg atctggtcgc agtcatctac ctgtaccgcg ccaaccacta aaccattacg
     2701 ttcagtaacc ttaatggaga taaccccttt cgtcgcacgc gacttggttg ggtattccgc
     2761 cactgcggta cgtttaccgt aaccgttttg cgttgcggtg aggattgcgc catcgccacg
     2821 aggcacgatc agagagacga ctttatcgcc ttcacctaag cgaataccgc gaacaccggt
     2881 ggtgttgcag cccatcgcac ggacagaaga ctctttaaag cgcaccactt taccttcagc
     2941 ggagaacagc attacttcgt cttcgccgct ggtcaggtca acgccgatca gctcatcgcc
     3001 gtcaaccagt ttgatcgcca ctttaccggc ggtacgcaga cggttgaact cggtgaggac
     3061 agttttcttc acggtaccgt tagcggtcgc catgaagact ttcacgcctt cttcaaactc
     3121 ggtcactggc aggatcgcag tgatacgttc gtcctgctcc agcggcagca ggttgacgat
     3181 cggacgaccg cgcgcgccac gagtggcttc cggcaactga taaactttca tcgaatagac
     3241 gcgaccacgg ctggagaagc acagaatatg gtcgtgagtg ttcgccacca gcagtcggtc
     3301 gataaagtct tcttctttaa tacgtgcggc agatttacct ttcccgccac gacgctgcgc
     3361 ttcgtattca gaaagcggct gatacttaac gtagccctgg tgagagagcg tcactaccac
     3421 atcttcctga gtgatcagat cttccaggtt gatgtctgcg ctgttggcgg tgatttcagt
     3481 acgacgtttg tcaccgaact gttcacgaac cagctccagc tcttcacgga tcacttccat
     3541 caaacgatcg gcgctaccaa gaatacgcaa cagttccgcg atctgatcca gcagctcttt
     3601 gtattcgtcg agcagttttt cgtgctcaag gccggtcagt ttctgcaaac gcagatccag
     3661 aatcgcctga gcttgctgtt cggtcaggta gtacagacca tcacgcacgc cgaactctgg
     3721 ctccagccat tccggacgcg cagcatcgtc gccagcacgt tcgagcatcg cggcaacgtt
     3781 gcccagctgc cacggattag caaccagcgc agttttcgct tctgcaggcg tcggcgcatg
     3841 acggatcagt tcgatgatcg ggtcgatgtt cgccagcgcc acggctaatg cttcaaggat
     3901 atgagcacga tcgcgagctt tacgcagttc gaaaatagta cgacgggtca ccacttcacg
     3961 gcggtgacga acaaacgccg cgatgatgtc tttcaggttc atgatcttcg gctgaccatg
     4021 gtgcaatgcc accatgttga taccgaaaga aacctgcaac tgggtctggg agtagaggtt
     4081 gttgagcaca acttcaccga ccgcatcgcg tttcacttca atcacgatgc gcataccgtc
     4141 tttgtcagac tcgtcacgca gcgcgctgat gccttccacg cgtttttctt ttaccagttc
     4201 cgcaatcttc tcgatcaggc gcgctttgtt tacctgatac ggaatttcgt ggacgataat
     4261 ggtttcacga ccggttttgg cgtcaacttc cacttctgcg cgagcgcgga tatacacctt
     4321 gccgcgaccg gtacggtaag cttcttcaat accgcgacga ccgttaatga ttgccgccgt
     4381 cgggaagtcc ggccccggga tgtgttccat cagcccttca atgctgatgt cttcatcatc
     4441 gatatacgcc agacaaccgt tgatgacttc cgtcaggttg tgcggcggga tgttggttgc
     4501 catacctacg gcgataccgg aagaaccgtt caccagcagg ttaggaattt tggttggcat
     4561 gacgtcggga attttttccg tgccgtcata gttatcaacg aaatcgaccg tctctttttc
     4621 gagatcggcc atcagttcat gggcaatttt cgccagacgg atttccgtat aacgcattgc
     4681 cgccgcagag tcgccgtcga tggaaccgaa gttaccctga ccgtctacca gcatgtaacg
     4741 cagcgagaat ggctgcgcca tacggacgat cgtgtcataa accgccgagt caccatgggg
     4801 atggtattta ccgattacgt caccaacgac acgggcagat tttttatagg ctttgttcca
     4861 gtcattgcct agtacgttca tggcgtaaag tacgcgacgg tgtaccggct tcaggccatc
     4921 tcggacatct ggcagcgcac ggccaacaat gaccgacatc gcataatcca gataggagct
     4981 cttcagctct tcctcaatgt tgaccggtgt aatttctctc gcaaggtcgc tcatctaacc
     5041 gctatccctc tactgtatcc cggattcaaa ggtcgcaaat tataacacag ccgcgcagtt
     5101 tgaggtaaac ctatacgctt tattcacatc caatgcctga tatactcgtt tgtcttgcca
     5161 attacggagt agaagtgcca atgaatgccg aaaaatcgcc ggaaaaccat aacgtagacc
     5221 acgaagagat cgctaaattt gaagccgtcg cctcccgctg gtgggatctt gaaggtgagt
     5281 tcaaaccgtt gcaccgcatt aacccgctgc gtctgggcta tattgccgag cgtgctggcg
     5341 gtttatttgg caaaaaggtg ctcgatgtcg gttgtggcgg cggcattctg gccgagagta
     5401 tggcgcgcga aggcgcgacg gtgaccggtc tggatatggg ctttgagcca ttgcaggtgg
     5461 caaaactgca cgcgctggaa agcggcattc aggtggatta tgtgcaggaa accgtggaag
     5521 agcacgcggc aaaacatgcc gggcagtatg atgtggtgac ctgcatggag atgctggagc
     5581 acgtccccga tccgcagtca gtggtcagag cctgtgcgca actggtgaaa ccaggcggcg
     5641 atgtcttttt ctcgacgctt aaccgcaacg gcaagtcatg gctgatggcg gtggttggcg
     5701 cggaatatat tttgcgcatg gtgcccaaag gcacgcatga tgtgaagaag tttattaaac
     5761 cggcagaact gcttggctgg gtagatcaga caagccttaa agagcgacat ataaccgggc
     5821 tacattacaa cccgctcact aatactttta aactcggccc cggcgtggat gtgaactata
     5881 tgctgcacac gcagaataag tgaggttgtt gtttggccga gccaatgcct gatgcgacgc
     5941 ttgccgcgtc ttatcagtat ctacaaatgc tccccgtagg ccggataagg cgtttacgcc
     6001 gcatccggca accgtgccga ctagacagtg attaccattt caccgtcatc gacaaaaacc
     6061 ctgccgtctg ggcaaaatca tcactccctt tctgccacgc cacgctgccg cgcagggaca
     6121 ctcgctgact gatattgccc gtgactccca cttttatttc accccgtcgc ttcaccgcat
     6181 cgtcactgat agtgctgcca tcttcttcaa tttccgtcgc atggggatcg tgataataat
     6241 tcagatctaa tgttggtgtg acaccaaccg cggtacgcca ttcgctgtgt aaacccagcc
     6301 gcgtctgaat atcatccccc tgcgattgtg acacgcgcgc atggttagcg gcggtaaaat
     6361 catcctgctg cacgccctga taaatcacct gcgcctgcgg ttcaatcacc acaccacgcc
     6421 ccggtaacca ctgatacccg gcttccagcg aggcgataat ccccgacgag tggtaatgat
     6481 ctgtgccatc gtcttgttcg gaaacatcat tgttaaacca cgcgtattgc agccagctat
     6541 ccagccaggc cccctgcttc tgattaccgt gctgatacca gcttgaggtc agcccaacgg
     6601 cataaccgtg gttctggtta tcggcgcgag ttccggtcat attcgagcgg ctctcgccct
     6661 gattatcact gtagccacca accgccccaa gcatccactc gccatcatcg ccccagcgcc
     6721 cgctaaacag gtcaccgcta agctgcaccg tagaagtgtc ttcatgctga gccagttgcc
     6781 ctgccgccgt gtaatgataa cgtccgccga taacacgtaa attcagcgtc tgaccattgc
     6841 cacctgcatg atcgcgtcgc tccatcataa acgcctgatt tgccgcccgc agattattga
     6901 gataaccgcc aactttggca ttcagcaccg gctggtaagc ggggattgga tcgggtgttg
     6961 ggtcaggatc gggtgttggg tcagggtctg gatcaggtgg caatggtggc gttacttctt
     7021 gcgatcgcag ataccagtcg ttgttatctt ccaccagcgt gtagtcatat gctcccatat
     7081 tgacgtagcc gctgcctgcc agactgaact tcgcattgtt ttgaaactgc gtgggatcgg
     7141 ctgcgaaatc aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatggagt
     7201 taaccaccac agttgtgttg ccagcagtat taccgttcaa caccaattga tcgcttaccg
     7261 agtcatcgcc gtttaattcg ctatcgagca gtagcgtacc gccgccggta taatcaccgt
     7321 ttaccgtcag cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg gacgtattgg
     7381 tcagattgcc attaaccgtc acgtcggaaa gataggtcag gctgtcagtt tcaaggttgg
     7441 tattaagctc acctgtcaac gtcacgccat cggtggcatt caccagcgtc ccatcgccgt
     7501 taaacagtga ggcattaagg gcgacagaag tatcctgccc ggtcaggcgc aaaaccgtac
     7561 catcgctgat gtcgatagtg ccgctggaag tagcatcaat agattgaata tcctgatccg
     7621 cgccagtaac gaacgtcgcc ccatcaccga cccacagtga cgaagcataa ggaaggatat
     7681 ccgcgacatc gcctttcagg gtgccttctt cgacacgtac cgccgactgc gttgtaccgc
     7741 tggcggtcag ctccagcgta cccgcccccg ttttagtcaa tgtgctaccc tcatcctgat
     7801 gctgcccgct gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg
     7861 ccacttcacc gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag
     7921 tatcgacatt ggcagtaatc tccatcacgc tttcttgttg cttatcggta aagataacct
     7981 ggcggttata gtgggtatct ccaagctgtg agttgtcgct cacctccagc ataccggagg
     8041 cgatttgtgt tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgcccg
     8101 catcgaccac cacgtagacg ccatctttcc cgtcaccgcc gatatgcata tcgtttccac
     8161 ctgcaagatc taccacatcc tgactgccga taaccgttcc gctgctgccg ctgacttcaa
     8221 ggccatcgtt gtatgatgtg ccactctgcc aggtggagaa atcagagaga tcgagcacac
     8281 cgccgtttaa cacgatcgac tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc
     8341 cttccagcga aagcaccgca ccatcatcaa cgactatatc gccggttagc gccatcgact
     8401 gcgcccccga cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga
     8461 tggtgccagc aaaactgcct tgattaacag taacattgcc accagcatcg atatttaaag
     8521 tgccattctg aaagcccgtc aatgagtgca caaaggtttg ttgggtggag ccaacgttta
     8581 gttctgcctg attctggtac ttatcaatac tccctatcgt cagaccgtag cagtcttgcg
     8641 gatcgtcctg gcaatgcgta tcgccgacat tcatcaggga gttgctgcga cccagggtaa
     8701 cttcaccgtt ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca
     8761 gatcgccgga acctgttttg gtgatcaacc cggtaccagc aatagagtca acagctccgt
     8821 cattctctgt attgccaata accagcgttt ttccgtcggc aatatcaaag gtaacttcac
     8881 ttaagccgag atacataaag ccacccgccg cagtggaagg accatctcca tagcctgctg
     8941 cgctgttgtt ctcatccact aacacgccgc cgttctggct gtagctgtca tcaacagaaa
     9001 tatcaataag atagggagcc gtcgcgctat tggtatatat cgccccgcca taaccttcgg
     9061 cagtgttatt tgtaaaggcg gtgttattta ttatcgtata acctgaagga tgcttgctgt
     9121 cgctattatt atcggtaaca tcgattgccc cgccatcgcc atcactgtaa cttgttgatg
     9181 tatatgcctg gttgttatca aaaataacat cacttaaata aacgtcatta ttaatggtat
     9241 aaattgcgcc acctttgcca tcattagcga tgttattgcg aaacatggcg ttagtgacac
     9301 gtaaatcgac gacaccagta tcattagtac cagaagaata gattgcgcca ccatagccgc
     9361 ctgcgacgtt accagaaaaa ataacatcag taagattcag cgttgagttt tctttagcaa
     9421 atatggcccc gccgttatta tattctcctg taacggtgtt attggcaaac agagtcattc
     9481 cagtttcatt ttctggtaat agtgaaaatt ccgctccttg ttgcaaaaat acggctcctc
     9541 cgctggcgtt attggtcata tccgaaaaaa ccagccattg cccatcggcg acactccagt
     9601 cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca actcgctttg atatcatatc
     9661 cctggcataa atcaatggcc gccgcgaaac cgttagcaga gaaaagagat gcaatcattg
     9721 acgggagtag agataaatac tccttgcgta gaaagataat ccgcatattc atctaaccat
     9781 cattttatat aagacggcat atttaaacgc attatacaat atgggttatc tttcttttgt
     9841 aaattgtcca acccgatgca atggctattt tgttattatt tgatgaatag tatcagtgcg
     9901 tcataat
//
LOCUS       DAZFWL020000161         9848 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFWL020000161 DAZFWL020000000
VERSION     DAZFWL020000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.161"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..136)
                     /locus_tag="ACHKNP_RS21395"
                     /old_locus_tag="pgaptmp_004279"
     CDS             complement(<1..136)
                     /locus_tag="ACHKNP_RS21395"
                     /old_locus_tag="pgaptmp_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706333.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     E"
     gene            214..1308
                     /gene="ddlA"
                     /locus_tag="ACHKNP_RS21400"
                     /old_locus_tag="pgaptmp_004280"
     CDS             214..1308
                     /gene="ddlA"
                     /locus_tag="ACHKNP_RS21400"
                     /old_locus_tag="pgaptmp_004280"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU4706334.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1332..1544)
                     /gene="yaiZ"
                     /locus_tag="ACHKNP_RS21405"
                     /old_locus_tag="pgaptmp_004281"
     CDS             complement(1332..1544)
                     /gene="yaiZ"
                     /locus_tag="ACHKNP_RS21405"
                     /old_locus_tag="pgaptmp_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HGU4706335.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1804..2112
                     /gene="yaiY"
                     /locus_tag="ACHKNP_RS21410"
                     /old_locus_tag="pgaptmp_004282"
     CDS             1804..2112
                     /gene="yaiY"
                     /locus_tag="ACHKNP_RS21410"
                     /old_locus_tag="pgaptmp_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HGU4706336.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2171..3265)
                     /gene="yaiW"
                     /locus_tag="ACHKNP_RS21415"
                     /old_locus_tag="pgaptmp_004283"
     CDS             complement(2171..3265)
                     /gene="yaiW"
                     /locus_tag="ACHKNP_RS21415"
                     /old_locus_tag="pgaptmp_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HGU4706337.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3278..4498)
                     /gene="sbmA"
                     /locus_tag="ACHKNP_RS21420"
                     /old_locus_tag="pgaptmp_004284"
     CDS             complement(3278..4498)
                     /gene="sbmA"
                     /locus_tag="ACHKNP_RS21420"
                     /old_locus_tag="pgaptmp_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HGU4706338.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4850..6007
                     /gene="ampH"
                     /locus_tag="ACHKNP_RS21425"
                     /old_locus_tag="pgaptmp_004285"
     CDS             4850..6007
                     /gene="ampH"
                     /locus_tag="ACHKNP_RS21425"
                     /old_locus_tag="pgaptmp_004285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HGU4706339.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMVPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6008..6631)
                     /gene="iprA"
                     /locus_tag="ACHKNP_RS21430"
                     /old_locus_tag="pgaptmp_004286"
     CDS             complement(6008..6631)
                     /gene="iprA"
                     /locus_tag="ACHKNP_RS21430"
                     /old_locus_tag="pgaptmp_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HGU4706340.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6719..9416)
                     /locus_tag="ACHKNP_RS21435"
                     /old_locus_tag="pgaptmp_004287"
                     /pseudo
     CDS             complement(6719..9416)
                     /locus_tag="ACHKNP_RS21435"
                     /old_locus_tag="pgaptmp_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556531.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(9446..>9848)
                     /locus_tag="ACHKNP_RS21440"
                     /old_locus_tag="pgaptmp_004288"
     CDS             complement(9446..>9848)
                     /locus_tag="ACHKNP_RS21440"
                     /old_locus_tag="pgaptmp_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4706341.1"
                     /translation="SRAVIGWSMSPRMTAQLACDALQMALWWRKRPRNVIVHTDRGGQ
                     YCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATV
                     FNYIECDYNRWRRHSLCGGLSPEQFENKNLA"
BASE COUNT         2553 a         2479 c         2315 g         2501 t
ORIGIN      
        1 actcaccgca tggccattta agccaggaat aaattagctt cccgttcatc atttttgtga
       61 tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac gctttgtcag
      121 cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta
      181 tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt
      241 tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat
      301 gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca agggcaatgg
      361 cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgtta
      421 cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca gcttatcgac
      481 gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg
      541 ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gttcgtaggt
      601 tctgatgttc tggcttcagc agcctgtatg gataaagatg ttaccaaacg tctgctgcgc
      661 gatgccgggc tgaacattgc gccatttatt accctgacgc gcgccaatcg tcacaacatt
      721 agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc ggctaatcag
      781 ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat tgccgtcgat
      841 ctggcgttcg aattcgatca taaagtgatc gttgagcaag ggatcaaagg tcgtgagatc
      901 gaatgcgcag ttctgggcaa cgacaacccg caagccagca cctgtggcga gatcgtactc
      961 accagcgact tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta
     1021 gttccggcag ccattgcgcc agaaatcaac gataagatcc gggcgattgc cgttcaggct
     1081 tatcaaacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagaaaac
     1141 gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag tatgtatccg
     1201 aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt gattgagctg
     1261 gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata ttttaacgcc
     1321 cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat aatcagcccc
     1381 gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg ccaacctttt
     1441 gcttcaaatc ccagtaaccc caccacgcca ttaagaatga atataaggcc aatggcgaac
     1501 gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc tccgttaacg
     1561 aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa cattttcgta
     1621 attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag attcacgctt
     1681 cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta aatgacctgt
     1741 cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact tccggaggtt
     1801 tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct
     1861 tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg ggctggggcg
     1921 caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat gcaggtccag
     1981 gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt cgggctgatc
     2041 ccgtttttag taggctgcct catttttgca gtggtggcgc tatggctgca ctggcgacat
     2101 cgccgccagt aagatttacg ttgccagata cggcctaaaa cgccttatct ggcatgacat
     2161 tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc
     2221 aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt
     2281 cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc ttgtacagcg
     2341 ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt tcgctgtcgc
     2401 tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg ggttctttac
     2461 tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgactg
     2521 cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaagcggt
     2581 atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc
     2641 cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac ggataccctt
     2701 tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg cgcacaggat
     2761 taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg tcaaaaatcg
     2821 cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt ttgccgtttg
     2881 gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc cgttcagcac
     2941 gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga tctgcctgat
     3001 aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc gtcggtgcca
     3061 gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg tctttcacgc
     3121 tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg ccctttttca
     3181 acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg
     3241 aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt
     3301 cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg tagatagaca
     3361 tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg cgaacctgac
     3421 caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca acaattgacg
     3481 gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatgt
     3541 tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg ctaaacagct
     3601 cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc agctctttac
     3661 ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg atccctacca
     3721 ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc agaccatacg
     3781 gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc agcaccggca
     3841 gaaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc tccagcgttg
     3901 aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata tgacgcagtt
     3961 gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac acatagtgac
     4021 tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc cccagaaaga
     4081 cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt agcgcggttt
     4141 gaatcagatc atagaacggt gcataccagg cgttgacggc gaccccgact tccaccaaaa
     4201 accaggtgac gaagatgatc agtgcagtac cgagtattga ccagtattgc caacggtgcg
     4261 ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa
     4321 aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc
     4381 cggtgatacg cgccacccag tcacccccac cggcttgcca gaagataacg gcaatcaatg
     4441 cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac ttaaacatat
     4501 gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccgcta
     4561 caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct tagttcgcca
     4621 gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt acataagcgg
     4681 attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac
     4741 gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga
     4801 gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag
     4861 tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg cggcgcagcc
     4921 gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc atatttttta
     4981 cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg
     5041 cagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt cggtcgtgcg
     5101 tattgcttcc ctcaccaagc taatgaccag tgaaatgctg gtgaaattgc tcgaccaggg
     5161 gaccgtgaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtgccaac
     5221 ctacaatggg acgcccatta cgctggtcaa tctggcaacc cataccagcg cgctgccccg
     5281 tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc gcgagcaacg
     5341 ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg cggcgtactc
     5401 taaccttgcg ttcgacctgc tggctgatgc actggcgaat gcgtccggca agccttatac
     5461 ccagttgttt gaagagcaga ttacccgtcc attggggatg aaagacacca cctacacccc
     5521 ctcaccggat cagtgccgcc gtttaatggt tgcagagcgc ggtgccagtc catgcaataa
     5581 cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata tgatgcgctg
     5641 gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag atcgcatgca
     5701 gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg tccccggcaa
     5761 agcggatgcg cttggtttag gctgggtata tatggtcccg aaagagggtc gtccggggat
     5821 tattcagaag acaggaggtg gtggcggttt cattacctat atggcgatga tcccgcagaa
     5881 gaatattggc gcgtttgtgg ttgtcactcg ttcgccgttg acgcgcttta aaaatatgag
     5941 cgatggcatc aatgacctgg tgaccgagct aagcgggaat aaaccgctgg ttatccccgc
     6001 gtcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt
     6061 cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa cgcgatatgc
     6121 gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct tcattccagt
     6181 caatcattga taataatgtt gcgcggattt gttcgtagga attatgccca atgagctgca
     6241 cgtcgcgtaa ttccagaatt ctattttgcc aggctaacca gtaaaaagcg tctcgccaga
     6301 gttgattttg ttcaataagc gtaatggttt gtttggctgg tagatgatat cccgtacaat
     6361 ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca gccagcccca
     6421 taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc agagagataa
     6481 ctccctctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat ataacttgct
     6541 tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg agcttaccaa
     6601 attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta atgcccgtca
     6661 aatccttgac gggcattatt tagattaaat taccagaatt tcttcggagt gaagaatatt
     6721 accaggtata tttaacaccc acgttcgcgg accagtcttg atctacgtca ccaccaccga
     6781 ggtagttagc atcggtatag gcgctgaagt tcttggtgaa gctgaactga gtacccagac
     6841 caacacgtac cgcagaccct tcagtaccgt tatcgatgga atcaccgttc acatcgttat
     6901 cgttgttaga gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga gcctggtctt
     6961 cgctgtaggt gaaggtataa cctgcatcta cacccagttc ataacgcatg ctgtcgtaag
     7021 actgaccgtc aactttcatg tcgttgctca gctggtagtc atcaccagac tggaacagac
     7081 cagaaacgct gccgtaagga gtcacgtaac cagcatcacc cagtttgaag tcgtaaccgg
     7141 ctttcaaacc gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa gtaccgttgc
     7201 tcatggttgc agacaggtcg ttgttgaagt gagagtagct caagctacca tcaacaaaga
     7261 cgttgttcgc gaagtgagca gaagagtaga tgtaggcagt ctggctgtct tgatccacct
     7321 gaccagaacg gtcattcatg tcacctttag cgaagcctgc agccgcaccg acgatccact
     7381 tagcgttgtt accgtcaatt ttggtatcaa caccgaccat gatgccgtta acatcctgat
     7441 catagttgat ggtgccgttg tcgccgttga agttaccacc gaagtagctt acccatgcgc
     7501 cgccgttatc agccaggcca tgacgagagt tggtcagacg agtaccaacg gtgtcttgtt
     7561 ccaggttcca gatattggtg ttcgcagacg ggatgctcag cgccatgtta gcgtagtcgg
     7621 tcagctccat ctgttgcaga acaacggtgt taccgcgctg ttcagcctga taggtgtatg
     7681 cacccaggtc agctttgtta gcagcagaga aggtcgcgtt ggtgttgacg tcgtttacgt
     7741 agataatttc tttatctttg taatcagcga tagcgccagc gcctgttgcg ttgtcgatac
     7801 gaactttgta gttaccggtt gcagcaacaa cgttgtcaac agagctgtta tccagttcgt
     7861 cgccgttgtt catgtcaccg ttaccgttga tagtcagatg accatcagag ttcatagcaa
     7921 caataccgta gccgtagtta gacttggaag tatcccaggt acggtcgttc accaaatcag
     7981 cgttcagaac ataatcgctg ctgtggatat cgaatacacc agacactacg ttgccgttat
     8041 ggtcagcgat gttgccgctg gtcagttcca aagtatcagt ctggaacgga ttatcatcga
     8101 actggtctac attaatatcc agtaaaccac cgttagtcac agttattgta tttgctaaca
     8161 gcgacggagt gtaataacca ttatagatac ttgaattgtc atcactagac aacgctacag
     8221 tactgtagct atcaactgtc agatgatcag ttccaacata accttcatct ccaatgttca
     8281 aagaagaacc attggtcaga gcgatgctat cagaaatcaa gctagattca gacacattaa
     8341 cctgagagcc attattcaca gctaaagtat caatgtttga agcgttaacg gtattccatt
     8401 cagaaccatt attcagagtg acgttaaaca caccgctctg gaatacttga tcatcaatcg
     8461 tacgacccca agcatttgct tctgttacaa cgcccggata caggctatta cctgtaacat
     8521 cataccagtc agtcgaagca gtagattcat catcaacatt tgctgatgta gtagctgagc
     8581 ctacccactt agaaccatta tccagagtga tattcagttc atcaacatta agtgagtcat
     8641 cagcccagcc accattggtg tccaagtacg ccatcggcat tactgtcatg accttgagga
     8701 tagaagtttg cgttgaaagt acttgcgaaa acaacatcac ccagcagagt agaattgctg
     8761 aaataaacat tgtttttcat ggagtaattt gaagccgcac tatcactgaa atacagagct
     8821 acatcattag cttcgccgtt accagagtaa tcactaggtt ccgcagagtt accatagaaa
     8881 ccactgtctt ccagagtagt tacagaacct gaagtcactg tggaatcggt aactgagata
     8941 ttgttatccc aagtatgaga ttcagtagtg taagtattgt ttacggtatt accctgagtc
     9001 aggctgatac cagcaacgcg ggagttgtta gaaatattga tgttactttc gacatcaaga
     9061 ttcactgcca gcccaaggcc aacaccaccg tttgcaaaca aaccggcgta ttcttcacct
     9121 tcaccagctg gggtatggaa ggtttcgtta gtatatttgg taacgtcacc gtccagatat
     9181 gaatcagtga agtataaacc ttcataatcg tcatcaattg tagaattagc aatatttaaa
     9241 gtgaatacat caccatcatg ccaattatca tcaattacac gatcagtacc gtattcagag
     9301 aactgataga cataatgacc atcactgctt tcacccatag gcagcattga agtgattgaa
     9361 ccatgaatca gggagttgct gatgttcaac tggtttgttg ttccatcagt aaagttgatc
     9421 ctacccacgt aatatggaca caggcctaag cgaggttctt gttttcaaat tgttccggac
     9481 tgaggccgcc acacaaactg tgccgccgcc accgattgta atcacattcg atataattaa
     9541 ataccgttgc ccgcattatt tcccggctga taaagtgttc tccatggata cattccactt
     9601 tcagcgaatg aaagaagctt tccacgcagg cattatcgta gcagcaacct tttgcgctca
     9661 tacttccacg cagattatgc cgcttcagtt gcgcctgata atctgctgaa cagtactggc
     9721 ctccacggtc cgtgtgaacg ataacgttcc ggggcctctt acgccaccac agcgccatct
     9781 gcagggcatc gcaggccagt tgcgccgtca tgcgtggcga cattgaccag ccaataacgg
     9841 cacgtgac
//
LOCUS       DAZFWL020000162         9721 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFWL020000162 DAZFWL020000000
VERSION     DAZFWL020000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.162"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..543
                     /gene="ykgH"
                     /locus_tag="ACHKNP_RS21445"
                     /old_locus_tag="pgaptmp_004289"
     CDS             <1..543
                     /gene="ykgH"
                     /locus_tag="ACHKNP_RS21445"
                     /old_locus_tag="pgaptmp_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HGU4706342.1"
                     /translation="INKDNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQR
                     ITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDE
                     IQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIV
                     ISVMFCLVKKMVDEYQQNSG"
     gene            complement(789..1484)
                     /locus_tag="ACHKNP_RS21450"
                     /old_locus_tag="pgaptmp_004290"
     CDS             complement(789..1484)
                     /locus_tag="ACHKNP_RS21450"
                     /old_locus_tag="pgaptmp_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HGU4706343.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1477..2904)
                     /locus_tag="ACHKNP_RS21455"
                     /old_locus_tag="pgaptmp_004291"
     CDS             complement(1477..2904)
                     /locus_tag="ACHKNP_RS21455"
                     /old_locus_tag="pgaptmp_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HGU4706344.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2915..3634)
                     /locus_tag="ACHKNP_RS21460"
                     /old_locus_tag="pgaptmp_004292"
     CDS             complement(2915..3634)
                     /locus_tag="ACHKNP_RS21460"
                     /old_locus_tag="pgaptmp_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HGU4706345.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4161..5015)
                     /gene="rclR"
                     /locus_tag="ACHKNP_RS21465"
                     /old_locus_tag="pgaptmp_004293"
     CDS             complement(4161..5015)
                     /gene="rclR"
                     /locus_tag="ACHKNP_RS21465"
                     /old_locus_tag="pgaptmp_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HGU4706346.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5241..6566
                     /gene="rclA"
                     /locus_tag="ACHKNP_RS21470"
                     /old_locus_tag="pgaptmp_004294"
     CDS             5241..6566
                     /gene="rclA"
                     /locus_tag="ACHKNP_RS21470"
                     /old_locus_tag="pgaptmp_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HGU4706347.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIAYGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            6675..6911
                     /gene="rclB"
                     /locus_tag="ACHKNP_RS21475"
                     /old_locus_tag="pgaptmp_004295"
     CDS             6675..6911
                     /gene="rclB"
                     /locus_tag="ACHKNP_RS21475"
                     /old_locus_tag="pgaptmp_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HGU4706348.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6923..7516
                     /gene="rclC"
                     /locus_tag="ACHKNP_RS21480"
                     /old_locus_tag="pgaptmp_004296"
     CDS             6923..7516
                     /gene="rclC"
                     /locus_tag="ACHKNP_RS21480"
                     /old_locus_tag="pgaptmp_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HGU4706349.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7676..8545)
                     /locus_tag="ACHKNP_RS21485"
                     /old_locus_tag="pgaptmp_004297"
     CDS             complement(7676..8545)
                     /locus_tag="ACHKNP_RS21485"
                     /old_locus_tag="pgaptmp_004297"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGU4706350.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            8794..9651
                     /locus_tag="ACHKNP_RS21490"
                     /old_locus_tag="pgaptmp_004298"
     CDS             8794..9651
                     /locus_tag="ACHKNP_RS21490"
                     /old_locus_tag="pgaptmp_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4706351.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
BASE COUNT         2705 a         2311 c         2072 g         2633 t
ORIGIN      
        1 atcaataaag ataatataaa agtgagttac agcctaaaaa taaaccagac aacaccaggc
       61 atacttgtta actgtgatag caataataac tttgcctgtc agaccacaat gactgaagat
      121 gttatacagc gaattactac attttttcac accagcccag atgtcaagaa cagagaaata
      181 aagctggaat ggtcaggaga taagagagat ttaccaactg ctgaagcgga aatatctcgc
      241 gtgcaggcgt ctattatcaa atggtatgcg tcagaatacc ataatggcag gcaagttctc
      301 gatgagatac aaacaccttc agcaattaac agtgagcttt atacaaaaat gatatacctg
      361 accaggaact ggtcattgta tccgaacggt gatggctgtg taactataag ctcaccagaa
      421 ataaaaaata aataccctgc tgccatttgc ctggctctgg gattttttct aagcattgta
      481 atttctgtaa tgttttgcct tgtcaaaaaa atggtagatg aataccaaca aaactctggg
      541 tagtaataaa cagcattctg atagagagtg cctgtataaa gctgaagtga caatgaccgg
      601 gaagggaacc cgttttcagg aatggatgcc tgtctttttc attgtaaata tcctggcatt
      661 actaatgcca ggatatttac agaagagcag cataaccatg acggaacggg gttaagtcag
      721 gttgtcctta acgctatttt caacctcgta tgccttcttc aggtttatgt tcagatctca
      781 tatctctctc agcaatcctc aataatcaga tacaccgctt tcaccggacc atgaaccccg
      841 acgactttga taagctcaat atccgccgtt gaactagggc cgctaatgat gttaatgcag
      901 gacggcattc gttcaccggc ctgcgctttc tgatgcaatt tttctgcgag ttgcgctaca
      961 cgcggcaaga tagtgctttt acgcaggata aaaagagaag attccgggag caggctcaat
     1021 gaacgcccgc gcccggcggc ggaaaaaaga accacgcctc ccgattcggt taaaccatat
     1081 tcagcataca cgacacccac tttagcctgc tcggcctgcg agatattctc ggcacctttc
     1141 gccggatccc aaacaacggc attgcattcc tgctgcaaac gttcactaat ccccaattcc
     1201 tccagcctcg tgtcaccgct aatcacgacc gactgatctc ccagctcttt acacagacgt
     1261 attgcagctt ctgccgcctt cgcctcgctg gtcagctcac agcgcgtcaa cataacatcg
     1321 ctggcaaact gaataaacgc gtcacagcgc tgctgttggt taagttgggt aagccgctcg
     1381 ttagcatagt tgttaagcgg cgcatcttct gcttgcggtt caagtcgcag cgggcgaccc
     1441 agtgcctgag caacgttatt caaaaattcg cctcgattat ccattctttt tctcctgcgc
     1501 ctgatgtttc ttaaaccaac tacggaaact ctctccgtca gcttcaggaa gatcgcgtgc
     1561 ttccatccag tcgctaatcg cgccaaattt gagtggtgtt ttgccgccat tgataaacca
     1621 gcttgccgca tgagcgccgg ccatcatccc gactttccac aatcctggat gactattggc
     1681 ataagcgaac atttttatcg cccgttgctc tgcttttgcg gtgatacctt tttcagccat
     1741 cacccgacga tgacgcaaaa tcagttttga cagcggaata cgcaccggac acacgctgtc
     1801 acaagcagtg cataaagagc aggcgtaggg taaatcttta aaatctttat agccgccaag
     1861 tagcggagaa atcaccgcac caattggccc tggataaata gagccatatc catgaccgcc
     1921 aatatggcga tatgccggac aagtattcat acaagccccg cagcgaatac agcgcagcac
     1981 atcccgaaat tcagaggcca gcacctcaga acgcccgtta tcgacaataa ccagatgaaa
     2041 ctcttcagga ccatcaacgt gccccgcttc gcgcggtcct gtcagccagg tgttgtatcc
     2101 cgtcaaacgt gcaccaacgg cactgcgcgc cagcatggtg atcaatacat ctacctcggc
     2161 aaacgtgggg gcaatacgct ccattcccat cactgcaata tgcgttttag gcagcgtggt
     2221 acacattcgc gcattacctt cattggtcac caggcatacc gaacctgtct ctgccaccgc
     2281 gaaattacag ccggtaatac ctatttcagc actgaggaaa tcttcgcgga ttttttgccg
     2341 gatgaataag gtcatcgctt caggcgtttc cggcccctca tagcccagac gttcgtgtag
     2401 cactcgacgg atctgatggc gatctttatg aattgccggg accacaacat gagatggcgg
     2461 atcttgatcc agctggagaa tgtattcacc cagatcggtt tcaatcacct gaatgccagc
     2521 atcctgcaac acatgattga caccaatctc ttcggtcacc atcgatttag atttcaccac
     2581 cttccgggca tttttgcgtt gggcaacctg taaaatgtag cgagtggcgt cttctttagt
     2641 ttttgcaaaa tagacgtgac cgccgttttg cgtcactttt tctgagagct ggtacagata
     2701 agcgtcgaga ttacttagaa catgatcacg tatctgggcg gcccgatcgc gccactcctc
     2761 ccagtgcccc aattcatcga ccattttttg ccgatttgcc ccaatacgct gctgcgcgtt
     2821 tgccaccgct ttgcgcatga tcggatcttc aatttgctga cggatgcgtg tcttaaaatc
     2881 tgtattactg gttttgatcg acatctttat atcctcagcg gctcatcaac acttcagcaa
     2941 tatgcatcac tttgactttc tgcccttccc gttgtaatcg cccactgatg ttcagcaggc
     3001 aactcacgtc agcaccaatt aaatactcag ggcggacttc catcaggtgc acaacctttt
     3061 ctttcaccat ctcgccggat atttcggcca ttttgaccga gaacgtgccg ccaaatccgc
     3121 agcaggtatc ctgttcagca aaggtcaaca gctccagtcc acgcacattt ttcagcagcg
     3181 taagtggctc gtccttcact cccagcttac gggccaggct acaagatggg tgatacacag
     3241 ctctcccttg caaactggca cctacatcga ctacccctaa tttattaaca ataaaagagg
     3301 tgagatcctg catacgcgcg gcaacctttt cggcacgtga tgcccattca ggttcatccg
     3361 ccagatacgt cgggtaactt tttacggcat aggtgcaaga gccagccggt gaaataatgg
     3421 gatcgtcgtt atcctccagt gcggcgatca gatttttcat ccctggaatc gcttctttga
     3481 tataaccgct attgatcgca ggctgaccgc agcatccctg tttctccggg aaatttacgc
     3541 gacagccgag tttttccagt agcagcacgg agtctcgtgc cattcttgat ttcagggcgt
     3601 caccaataca ggtgacaaag aaattgacat tcacaactaa tactccatta cttcatgccc
     3661 atttatgccc gatgtttccc ccccaccggg ggatcaatca tcaataaata tggcgttctt
     3721 ttttaattgg ctagcaacat accacccctc atttgtatga caaatgataa tctttcatcc
     3781 aaacttcaaa tgcaataact aattttattt ctttcggcat cgctcacatt ttatggggaa
     3841 atcgacgttc atttttggca taagaaatcc atttaaaaag gctacatccc ttatacctat
     3901 tcagagctac ttattcaggg gttattgaga tatacactga tggtgtacaa aaaaatcacc
     3961 gacaataatc acgtatcaag taatagtcgg ggttttattt aattgaagca atttagtttg
     4021 catttttatt taaaaactga gctagagtcc atcagaaaaa aatatataaa gcccaaacca
     4081 ccgttggaaa tagaaatagt tgaaaggctc acaactcccg gaatgcggat tatctttgcc
     4141 ctgatttctg gcggttttat tcagggtgca agctgtctga ccctttcccg atattctccc
     4201 ggagtacaac caaactcgcg gacaaacgcc ttgtgaaaag atgattcact ggcatagcct
     4261 actgactcag cgatcaccac aacagggagc atttcccggg aaaacatctg ggccgctatt
     4321 tgtagacgca actttgttaa tacagccagc ggcgtggttc cggaaacatc acggaaaagc
     4381 tgggcaaaac ttgcccggga catgtgagcg atgctggcca gcgattcgac ggtccaggcg
     4441 tgtcctggca tttccagcat ttgctgtatt accgcaccaa gacgtggatg cagaagcaaa
     4501 ctgagaatgt ttttctcagt attaacctgt gcaatccact cacgcaccgc gagggtaaag
     4561 aatgtagcgc agatctggct acacagtgca tccactcccg gcatcgccga tctggattcc
     4621 tgttgtaaaa acgggatcgc ctccctcagc cagttatatt ccacgctgtg gttaaccggt
     4681 gctaaaaaca gcgtttccgg caaagacgtt aaaaaataac gcgccgaatg ttgcaaccga
     4741 agagtgccgc agacaataca ggtcgattca ttatcgacat gactcaggcg atgagctgaa
     4801 ttttgtggta gcaggaccac atttcccggg cgtaatgtaa aaatctctcc cgtcggcatt
     4861 tccagcttcg ccgctccttg cgttaacgca tgccaacgaa taaccgataa ttccccggca
     4921 ccatgtggaa gctgccagtc acttcctaac acgcaattct tatcgatcgt tccttgtgga
     4981 gcgttaagca tcaacaaacg gctaagggca tccatatcag actcctgagt ggtttttgat
     5041 tacaaacacc aacaatctta gtaaataact aatctaatac catcaaatgg ttaatcatga
     5101 tttttgtttt gtgattctga ttaagaaaaa gaaaatacca gacgataaga caaaatatca
     5161 agcgttgtga agaaatggta tttgctcttt tgcgtctata gtcatgatgt caaatgaacg
     5221 cgtttcgaca ggaaatcatc atgaataaat atcaggcagt gattattggt tttggcaagg
     5281 ctggaaaaac attagccgtc acgctggcaa aagcaggttg gcgagtggct ctcatcgaac
     5341 aatcaaatgc aatgtatggc gggacctgta ttaatatcgg ctgcatccca accaaaacat
     5401 tggttcatga cgcacagcag cacacagatt ttgtccgtgc catacagcgt aaaaatgaag
     5461 tggttaattt tttacgtaat aagaattttc ataatcttgc ggatatgccc aatatcgacg
     5521 tgatcgacgg ccaggcggag tttatcaata atcatagcct gcgtgttcat cggcctgagg
     5581 caaatctgga aattcatggc gagaaaattt ttattaatac cggtgcacaa accgtggttc
     5641 cgccaattcc tggaattacc accacgccag gagtatatga cagcaccgga ttacttaatc
     5701 taaaagaatt gcctgggcat ttaggtattt tgggcggcgg atatattggc gttgagttcg
     5761 cctctatgtt cgctaatttt ggcagcaagg taaccatttt agaagcagct tcgctgtttt
     5821 tgcctcggga agatcgggat attgctgata atatcgcgac gattttacgc gatcagggcg
     5881 tcgatattat cctcaatgcc catgtggagc gaatcagtca ccatgaaaat caagtgcaag
     5941 tgcatagcga gcacgcccaa ctggcggtgg atgcactgtt aatagcttac ggtcgtcaac
     6001 cggctaccgc ttcgttacat ccagaaaatg ccggtatcgc agtaaacgag cgcggggcaa
     6061 ttgtcgttga caagcaatta cataccaccg cagacaatat ttgggcgatg ggagatgtta
     6121 ccggcgggct gcaatttact tatatttcac tggatgatta ccgcattgtg cgtgatgagt
     6181 tgctgggaga aggcagacgt agtactgatg atcggaaaaa tgtgccttat tccgtattca
     6241 tgacaccgcc actgtccagg gttggtatga cagaagaaca ggccagagag agtggtgctg
     6301 atattcaggt ggtgacattg cctgtagctg caattccgcg tgccagagtg atgaatgata
     6361 ctcgtggggt attaaaagcg attgttgata ataaaaccca acgtatatta ggggcatcac
     6421 tgctgtgtgt tgactcccac gagatgatca atatagtgaa aatggtgatg gatgccgggc
     6481 tgccttatag catattacgc gatcagatat ttactcatcc gtcgatgagc gaatcactca
     6541 atgatctatt ttcattagtc aaataaactc aaaatcagac gccagaacaa atattctggc
     6601 gtctcagaga aaagaatctt attaattccc tgttactcta taacttctta atcacttcat
     6661 tgatggtatt ttatatgttt aaaaaatctg ttttatttgc aacactatta tctggcgtta
     6721 tggcattttc caccaatgca gatgataaaa taattctgaa acatatcagc gtctcgtcag
     6781 tatcagcatc accgacagtt ctggaggata ccattgctga tatagccaga aaatataatg
     6841 cttcatcctg gaaagtcaca tcgatgcgaa ttgataataa ttcaaccgca acagcagtat
     6901 tgtataaata aggatgttca caatggaaaa atacctgcac ctgttaagtc ggggagataa
     6961 aattggcctg acattgattc gtctgagtat tgcaattgtt tttatgtgga ttgggttatt
     7021 aaagtttgtc ccttacgagg cagacagcat tacaccattc gtcgcaaaca gtccactaat
     7081 gtcgttcttt tatgaacacc cggaagacta taaacagtat ctgactcacg aaggcgaata
     7141 caaaccagaa gcaagggcat ggcaaacggc caataatacc tatggttttt ccaacggtct
     7201 tggcgtcgtg gaggtgatta ttgctctgct ggttttggct aatcctgtca atcgctggtt
     7261 aggtttattg ggaggactga tggcatttac cacgccgctg gtaacactct catttttaat
     7321 caccaccccg gaggcatggg tacccgcatt gggtgacgct catcatggtt tcccttattt
     7381 atccggtgct ggtcgcctgg tattgaaaga tactctgatg ctggcaggtg ctgtcatgat
     7441 aatggcagat tcggcgcggg aaattcttaa acaacgcagt aatgaatcca gttcaacgtt
     7501 aaaaactgaa tattgataat ccgaacactg tctgttgttc actttttcag gcgtcaggcc
     7561 ccctttctga tgatgaagcc tgacgccata ttaccggaga tatgacagag tgaagatcaa
     7621 tatcactccc ctgttaaaag attcttgtcg tctcaggtaa aatatatgat taatatcaat
     7681 catgtatttt tacagcaaga caaccacgaa caaattcagg attaaaatgt tttaccgact
     7741 ccccaacata acccaaatca agcgaactaa tttgtgccat ttctttctct gtcagtgaga
     7801 aatcccagat agcaaaattt tcttcgatac gctgttgatg tgtcgatttc ggaataacga
     7861 ccactccccg ttgaatattc caacgcagaa tgacttgaga aatcgatttt tgatgggcat
     7921 ctgcaatact ctgaagcaga ttattttcaa agggtttatg tcgtccacca cctaacggag
     7981 cccatgcttc aggctgcacg ttataatact tcatggttgc cagcgcctct ggttgggcaa
     8041 aatagggatg caactcgacc tggttgacca tcggtttaac tcttaccgtt tcacaaaagt
     8101 tcgccagaac atgaggatag aaattggaaa ccccaattgc ttttaatttg ccctcttcat
     8161 aggcatcttc gagtgcacgc cacgcactga aataatcgcg catagcctgg tgcaataaat
     8221 agaggtcgaa gtactctagt cccgattttt ttaatgatgc ttcaatacct gctgctgccg
     8281 tatcctgatt caacatatcc tgcacccaca atttagacgt tataaataac tcatctcggg
     8341 tacataaacc ttcagatata gcttcacgaa cggcttcacc gacggcatct tcattaccgt
     8401 ataccgctgc agtgtcgatg agtcgataac ctgtgcggat agcgttcaac actgactgtt
     8461 tgcacacgtt tttatcggta acctgaaaaa caccaaatcc catcatcggc attttcagat
     8521 tgttacttaa tacggaaaat tccactgtca gtccctccat taggcatgaa caatgagtct
     8581 acgttaaaac gtaacctcaa attagtacgt ggattttgat atcacttatg caaaaaattc
     8641 attaataatg caggactgaa acctctctat tttcggggac aacgaagcag acgctaccag
     8701 tgcttttgcc ttcgcccttg ctgtttttga tacacttagg gcccagggca taacgaaaat
     8761 gtgcgatatg acgaagaatc aatggagaat gtgatgatca ggcagaagat tctacagcag
     8821 ctcctggagt ggattgagtg caatcttgag caccctattt caatcgaaga tatcgcacag
     8881 aaatctggct acagcagacg caacatccag cttctgttcc gaaatttcat gcatgtgcct
     8941 ttgggagaat acattcgcaa acgaaggctt tgtcgtgccg ccattcttgt ccggctcacc
     9001 gcgaaatcta tgcttgatat tgcactctct ttgcattttg attcacagca gtcattcagc
     9061 cgcgaattta aaaagttatt cggctgctct ccccgtgtat accgccaccg tgattattgg
     9121 gatctcgcaa atatcttccc ttctttttta atacgtcaac agcaaaagac cgagtgtaga
     9181 ttagtcaact ttcctgagac acctattttt ggcaattcat ttaaatatga tattgaagtg
     9241 tcgaataaat caccggatga agaagtcaaa ctgcgacgtc atcatttagc cagatgtatg
     9301 aataatttta agacggatat ctatttcgtc tccacgtttg aaccgtcaac aaaatcggtc
     9361 gatttgctca cggttgaaac ttttgccggt acggtatgca aacaaaccga ttcagagatg
     9421 ccgaaagagt ggacaattaa ccgaggactg tatgcctctt tccgctatga aggtgagtgg
     9481 gagcactacc ctgaatgggc gcgaaacctc tatctgatgg aattacccgc cagggggtta
     9541 gccagagtga acggcagtga tattgagcgg ttttattaca atgaaaattt cgtcgaaatt
     9601 gatagcaata acattgtttg cgaaattttt atccccgttc gtccggttta gttttcgcta
     9661 tatctaaatg aatttataac gagactactg tcaggaaatt caataaataa tggcgttgaa
     9721 g
//
LOCUS       DAZFWL020000163         9223 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFWL020000163 DAZFWL020000000
VERSION     DAZFWL020000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.163"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..1119
                     /locus_tag="ACHKNP_RS21495"
                     /old_locus_tag="pgaptmp_004299"
     CDS             61..1119
                     /locus_tag="ACHKNP_RS21495"
                     /old_locus_tag="pgaptmp_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGU4706352.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYVIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            1129..2271
                     /locus_tag="ACHKNP_RS21500"
                     /old_locus_tag="pgaptmp_004300"
     CDS             1129..2271
                     /locus_tag="ACHKNP_RS21500"
                     /old_locus_tag="pgaptmp_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HGU4706353.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2264..3037
                     /locus_tag="ACHKNP_RS21505"
                     /old_locus_tag="pgaptmp_004301"
     CDS             2264..3037
                     /locus_tag="ACHKNP_RS21505"
                     /old_locus_tag="pgaptmp_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706354.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3039..4118
                     /locus_tag="ACHKNP_RS21510"
                     /old_locus_tag="pgaptmp_004302"
     CDS             3039..4118
                     /locus_tag="ACHKNP_RS21510"
                     /old_locus_tag="pgaptmp_004302"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HGU4706355.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4118..5074
                     /locus_tag="ACHKNP_RS21515"
                     /old_locus_tag="pgaptmp_004303"
     CDS             4118..5074
                     /locus_tag="ACHKNP_RS21515"
                     /old_locus_tag="pgaptmp_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HGU4706356.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            5085..6293
                     /locus_tag="ACHKNP_RS21520"
                     /old_locus_tag="pgaptmp_004304"
     CDS             5085..6293
                     /locus_tag="ACHKNP_RS21520"
                     /old_locus_tag="pgaptmp_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HGU4706357.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6311..6778
                     /gene="terW"
                     /locus_tag="ACHKNP_RS21525"
                     /old_locus_tag="pgaptmp_004305"
     CDS             6311..6778
                     /gene="terW"
                     /locus_tag="ACHKNP_RS21525"
                     /old_locus_tag="pgaptmp_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HGU4706358.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7039..7368
                     /locus_tag="ACHKNP_RS21530"
                     /old_locus_tag="pgaptmp_004306"
     CDS             7039..7368
                     /locus_tag="ACHKNP_RS21530"
                     /old_locus_tag="pgaptmp_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4706359.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            7355..7735
                     /gene="tnpB"
                     /locus_tag="ACHKNP_RS21535"
                     /old_locus_tag="pgaptmp_004307"
     CDS             7355..7735
                     /gene="tnpB"
                     /locus_tag="ACHKNP_RS21535"
                     /old_locus_tag="pgaptmp_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4706360.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQEEPEDAQSNAITQ"
     gene            7778..8608
                     /locus_tag="ACHKNP_RS21540"
                     /old_locus_tag="pgaptmp_004308"
                     /pseudo
     CDS             7778..8608
                     /locus_tag="ACHKNP_RS21540"
                     /old_locus_tag="pgaptmp_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8639..>9223)
                     /locus_tag="ACHKNP_RS21545"
                     /old_locus_tag="pgaptmp_004309"
     CDS             complement(8639..>9223)
                     /locus_tag="ACHKNP_RS21545"
                     /old_locus_tag="pgaptmp_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HGU4706361.1"
                     /translation="EREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYA
                     IEDEIRGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDA
                     LCYYSDDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTNK"
BASE COUNT         2196 a         2434 c         2426 g         2167 t
ORIGIN      
        1 acactcatag actcacttct caatcataaa tttgcatggc gttccggata attaaaaaaa
       61 atgaacaaga aaatttggtt tatggaaggt ttatcctccc agcgagatat tattcagggg
      121 gtaaaatcat ttgcacaaaa aaataatttt gccattaccg tttttgcctc ccaccgtaac
      181 gaaagaaatg aaatcctttc cgttgccgat tattctttga ctgaacctga agatcctcaa
      241 aaacgtcttc agtttatcca ggaaaccatt cagacctacg tcatccacca tattcatact
      301 ggccgtaaca gccagtggtt tgaagaacac cgttcagcca ttgaaccgac cggtgccacc
      361 cttactaccg gtgcaacggg cgtcgactgg ttaactctgg ctgacgaaaa agttactttt
      421 gctcagttta tggagcaaaa gggtctcccg gtcgtaccat cctggcgggt gaatacgctg
      481 gcagaattaa agacacacct cgcggccccg ccgttcactg acagcccggt atgcgtgaag
      541 ccggtgacgg gtatctatgg catgggattc tggcgctttg atgacagtgc ttcgcctatg
      601 gccgtcttta atcatcccga acatcgtctg gtcagtccgc aacagtatat tgcagcagca
      661 tcagctgctg agtcgtttaa accccttgtt ttgatgccgt acctgccagg cccggaattt
      721 tccgtcgata tcctcgcgga taagggcgaa atactcgcag ccgtgggacg ccgtaaggaa
      781 ggggctatcc agtatctggt aaacgaagga agcgcctggg aactggcgtg tgactgcgcc
      841 cgtgttatga aggccgacgg gctggtgaat gttcagacgc gaaacgatgt gaatggcaac
      901 ccggtgctgc ttgaaaccaa catgcgtccg tcaggggggg tgggttatac ccttcacagc
      961 ggtgtgaacc ttcctgggtt atttgctgcc tttaagctcg gtctgatgtc tgaagatatg
     1021 gtacgccaga gcgctaaaaa caccttttct ccggttgcgg tgagatccat tacggatgta
     1081 attgcatacc cggaatcact ctctaacctt ctgaattaag gcgtatttat gtctgatatc
     1141 ctcgaagata tgatttatcg ccgtaccctc tcatgcggta cgattcaggt aacccgtgac
     1201 cagggtgaag tttcgctcga tgacctgttt gacatcgctg aaagacgtaa cccgaaacgc
     1261 gcctttctgt ttgtcagcaa agtactcggc aggcacattc ccgtgccacc ctcagtcatg
     1321 cgacaggcct acagacagct tgccagccag ttcccctcga cactgacagg acccgtactg
     1381 tttatcggta tggctgaaac cgccgttggg cttggggccg gtgtatttga tgaagtgcgc
     1441 caccagcatc gtgaatctgt ctatctgacc tctacccgtc acccggttga tggcacgttg
     1501 ctttgcgagt tcaaggaaga acacagccac gccaccgatc atctgatcta tctgccagat
     1561 gatgaagaga aaagacgtcg tgttaccaac gcacgaacgc tggttttgat tgatgacgag
     1621 gcaaccaccg gtaatacctt tattaacctg ctttctgccc tgcgtaatac cggcaagctt
     1681 caacatattg aacaggttat cgccgtaacg ctgaccgact ggagtggcaa tgccctgtcg
     1741 gagcgcaccc ccttgcccgt cacttcggtt tcgcttgtaa gcggtcagtg gggatggacc
     1801 ccattacctg atgcacctgt cccggacatg ccgaaagtca acgtaacttc acgaggagaa
     1861 tgggacatcc aggggaaaca gtcatggggc cgactgggga tgctcgcacc tgcggccgat
     1921 ctcggccatg aggtctccgt ccacaagggg gaacgtattc tggttctcgg gaccggggaa
     1981 ttcgtctggg agccgttcct gcttgctgaa cggctcgaag ctgccggagc acaggcattt
     2041 tatggatcga ccacccgctc ccctgtcgcc gttggttatg ccattgagtc cgccatttcc
     2101 tttacggata actacgggct gggcatcccc aattttgtct ataacgtcgc ccaccagcag
     2161 tttgaccgca ttcttgtgtg tactgagaca cccgcagaaa gtattgacac gcagcttctt
     2221 aaagcgctgg ctgaggttgc gcccgtcgtg gagattgtta cctatgaata aacccgttgt
     2281 tctgagcgac cttgacgaca cgctctttca gacccgtcgt aaaatggtgg acgagctggc
     2341 cctggagcca ttccgtaccg gtgccgttga tcgcaccctg aatccacgaa gctttatgac
     2401 ggaagaacag tccatgttgg tggactggct cctggagcag gctgaactca tacccgttac
     2461 cgcacgggga actgaagaaa tcagccgcgt acggatccct ttccactcct gggcaatcac
     2521 aacgcacggg gccgtcattc tcacgcctga aggcaaaccc gacgaggagt ggaaggccca
     2581 tatgctcggc cagctggctc cctaccagga aaagctgaca tcgatgcagc gtctgatcac
     2641 tgaaatgatg gacgcaaaag gaatcaatgc ctgggcaagg ctgaacttcg aatacggtga
     2701 aacggcggtt tatatggtga tgaaacaccg cgacagcacc cgccttgacg agctcaatgc
     2761 cattgcagat gagatagaaa cggtgtttcc gaccgagggc ttctacatcc accgcaacag
     2821 taataacgtg gcctggcttc ccaccccggt tgagaaaggg ctggcagtca gctggctgct
     2881 tgaaaaactt cgggctgaac gaggagtttt ccccgtaatt ggtttgggcg acagcctgag
     2941 cgatcatcgt tttatgaaac tgtgcagctg gtttggcatc ccgcgtcaga gtcagtttgc
     3001 agatgccatt tcacagcgaa tttttggaga aaattaaaat gcagccacat gaaacattta
     3061 ccggctcata ccagcccggt gacgtggaat ttctgctaaa gccggttgtc attgagatga
     3121 cgccggttga gaaaaaagaa gaactgattc agtccgggaa gaaacattat tcggacatgc
     3181 tcagccagga gccagcgcct acacaatggc atcttgattt gtttcaccgg gcgctggatc
     3241 ggggcgcaga gaggcttgca aaagaagtca cacagctcgc tattgctctg gccgaacgct
     3301 tcggtgatga gcccattgta ctggccagtc ttgtcagggc tggcgttccg ctcggcgtta
     3361 tgctgcacca ggccctgcgt gacatgggga aaacctcatg gcattacggt atcagcatta
     3421 tccgggatcg tggaattgac ggtgcagccc tcgacgtcat tgaagagcgg catggtacca
     3481 gcggtattgt ctttgttgac ggatggacag gtaaaggcgc aattaccgga gagcttgtac
     3541 gcgcactgaa agatcgcccg ggctatcctg agcagccgcg actggttgtc ctggccgatc
     3601 cctgtggctg ctcctggctt gcagccagtg atgatgactg gctcattcct tttggcatca
     3661 tgggggcgcc ggtatcaggc ctgatctccc ggtcagtatg gtcctctgaa ggattacacg
     3721 ggtgcatggt ttgcgagcat ctcagtgaat tcgaatgcag ccggatgctt gccgataccg
     3781 tcgctcattt ccgtaagaag ttaacaccgt catccctcgc tcccctgagc tggaatacgg
     3841 aatcagcccg ggtcttatgg cagacaagtc gcgacgttat cgcgttcctg gccgatgaat
     3901 ttaaagtgga cagcgtcaat cgtattaaac ccggtattgc tgaagcaacc cgggctgtat
     3961 tacgtcgggt accggaccat gtatttgtgc gttctattga cgacccggac gttgccttgc
     4021 ttgtagggct tgctcgtgaa aagggaatag ttgttacaga aatgggggga accctcggcc
     4081 agtatcgggc tgtaaccatt atcaagaagg tactctgatg aaaaatcgtc tttctccctg
     4141 gaatctggga gccacgctat acatgcctgc aacacgggaa gatattgctg atgccgtcct
     4201 gcacgggaaa atcccgggct tgcgctctct ggtgatttgc ctggaggatg ctgtcagtga
     4261 agcagacatc cccgtcgccc tcaaaaatct ggagcacctg ctgcacgagc tcagtaacag
     4321 catgagtagc ctgggtaaaa atgactggcc tctggtgttc attcgccccc ggcatgccga
     4381 aatgggcaga tggttaaaag cgcattatga tctttccgct gtcgacgggt ttgtgctgcc
     4441 taaatttacc ctcagttcgt tagctgaatg gtgggatatc atggtcggga ctcacctgtg
     4501 catgatgcca acgctggaaa cagaagatgt ctttgacgtg gttcagatgc gcgagctggc
     4561 tacccgtctg gaggaacatc cctgccacga ccgcattatt gcgcttcgaa tcggcggcaa
     4621 cgatcttatg aacgttgtgt cgcttcgccg tccccgggac ctgacgctgt atgacagccc
     4681 gatgggctac gtcatcaaaa tgctcgtttc ggtcttcggc ccgcgtgatt ttgccctgac
     4741 cgcgcctgta tgtgagcata ttgacgatca tgccgttatg gccagagagc tggctcttga
     4801 tatggcacac gggcttgttg ggaaaacggc cattcacccg ggtcagatag aggtcattca
     4861 aaacgcgctg atggtcactc agggtgagca ttctgacgct ctgcggatcc tgaactctac
     4921 ccaggccgtg tttaagtcgc agggagcaat gtgtgaaccc gccacacatc gccgctgggc
     4981 ggctggcatt ctggacaggg ctcgttttta tgggttacag aacgagcaaa gcgctgatgg
     5041 aatcagatta cttaccgtga cccagcatca ttaaggaaat cataatgggg ttcaggttca
     5101 gaaagtcgat caacattatt cctggcgttc gcctcaacct gagtaacggt gcaccgagcc
     5161 tgagtgtcgg gccgagaggt gcttccgttt cttttggtag ccgggggacc tatgccaatc
     5221 tgggcttgcc cggtaccggg ctgagttacc gtacccggct tgaccgggcc gcgcgttcca
     5281 gaggtgaaaa ccggacggca accgacccgg ggctcagaca ggcgcttgag cagaaagccg
     5341 ctgaactcat gtcagcggta accgcaatcc gtaatattca cgagctgacg ccggatccaa
     5401 aaacaggcat cagctgggca gagctggaag cagtatacct gcataacaga acgtcgcctt
     5461 ttcaggttcc ggcaccggtg cgtccagaaa agccagacta ccttgtattg ccggaaaagc
     5521 ctgccgagag cgaaggcatt agttttttgg gtaaatggtt tgaatcggaa tcagctaaag
     5581 ctgagcgcca cgccgaaaat cttcgccggt ggcagcagga gctgattgat gtggagcgtg
     5641 agaataccct tcgacagcac cggtaccagc aacaacggac ggcctgggcc gaacagtatg
     5701 caaactggaa gtttgaagct gaagaacatg aaaaacggct cgccacggct caggcagatg
     5761 cccggcagca gttccggaca gacgccgcgt ttttcgaatc atacctggcg ggtgtgctgg
     5821 cagaaactga atggccgcgt gaaacgcttg ttgcatttga agtaaagccg gagctatcag
     5881 cagtcctgct ggacgttgat ttagctgaga ttgaagattt ccctgataag atttacggcg
     5941 ttaatgcccg gggaacggag ctgacggaaa aagccatgac gcaaaaagcc gtacgcgaaa
     6001 actatgccca ccacgtccat ggctgcttgt tccgcctggt cggtatcgtt ttacatacgc
     6061 tacctttcga caacgtgatt gtgtcaggct ttacgcaacg ggtcagtaag cggaccggct
     6121 atctggagga tgagtatatc ctgtcctgca aatgcactcg cagccagatg tcgtcagtaa
     6181 attttgcagg cataaaacac attgatccgg ttgaagcgtt aggcgatcaa ccggttattc
     6241 gaaagatgag cagtaccttc atttttcagc ctattgaacc actaaccctt taagcaggct
     6301 aaatcttacg atgcaattaa ataccagaca ggcccggatt tttaagcttg ccaacttact
     6361 gggcaccggc aagcccgttt ccgctgccga cattataacc agtctggaat gctctgagcc
     6421 gacattaacc cgggccctca aagagctacg cgagtcatat tctgcggaga tcaaatacag
     6481 caaggcaggt cattcctatc atctcgttaa tccgggccag ttggataaaa agaccctccg
     6541 ccggatgaat gaagcgctcg cacagaatgc tgaactgaaa accggtgagt ccacagggaa
     6601 ggtcgtactc gataaggata aaaaaacagc cgtgtcatta tcgctacgca tgcggattct
     6661 aagaaaaatt gaccgtctgg ctgcgctgag tgactcgacc cgaagtgaag cggtagagaa
     6721 gctggctctg cactccgttg atgaactgat aaaagaatac agcgctaaaa agtcctgacg
     6781 ctaaatctca gaccggggtg aaaactccgg ttttgtcata tttcacctct gtattggcgt
     6841 gatgcaaaat ggcatctgaa tcgatcatgg tttggcatcc atttccgatc acagtttggc
     6901 atcacaatcg atcacgattt ggcatgcttc cgctcattga ttggcatcct ggtgtcgtta
     6961 cggggaagtc atcttcacag ggatcttcag tcagttagca ttaccgacat ccattcacca
     7021 acttaccgga gataatcaat gtcaaaaccc cgatggacgc ttgatcagaa aaaacatcac
     7081 gttgccgcat ggcgcgccag cggccttacc cgtgaacagt actgtgaact ttacgacatt
     7141 ccgtttaaat ccctgcgcca gtggccgcag gatgtcgcaa aagccgaaaa acgagccaga
     7201 gcaccagaaa ttattcctgt ttcagtatct ggctcttccg gaatgactga tggcagacca
     7261 ttgtcggatg agcctgtcac tctcttcctg cccggtggta tccggatgtg ctgccagcct
     7321 tctcagttaa ccgatgtttt ccgggctctt cgccatgctg acgcctgata acgtattcct
     7381 ggtcgtaaaa cccgtcgata tgcgccgggg tatcgacaca ctgacacagt acgtccagaa
     7441 cgaacttaac gctgcatggc atgacggtgc tgcttttgtc ttcaccaata aagtccgctc
     7501 ccgcataaaa gttctccggt gggacaaaca tggcgtatgg ctgtgtaccc gacgactgca
     7561 ccggggaagc ttccgctggc cccggaaagg tgatgccaca tggcatctca cacaggatga
     7621 gtttcactgg ctggtctttg gggttgactg gcaacaggtg aaagggcatg acctggcaaa
     7681 atgggtatac caggaagaac ccgaagatgc tcagagcaac gcaataactc aatgaaatat
     7741 aatgaatatc catgcgtgat gtcatataat ccaccgcatg gatatctcgc ttctcagtac
     7801 caccagtgac cctgaacagc ttcgcgctct ggcaattgcc atggtgcaaa aagtcatggc
     7861 tgagaatgca gaacttcaga accgtatccg catcctggaa gagcagatga agctcgcacg
     7921 ccagcagcgt ttcggtaaaa aatgcgaatc gctggccggg atgcagcgtt ccctgttcga
     7981 agaggatgtt gacgcggata tcgcagaaat ctcggcgcat ctggacaaac tgcttcctca
     8041 gaccggggac gaagaaaaaa caacaacacg tcctgttcgt aagcccttgc cttcacctct
     8101 tccccgtgct gaaaaggtta tcccgccagc tgaggaacgt tgtcctgact gtgatgcccc
     8161 cctgcacttc attcgcgatg aagtcagcga gaagctggaa tatattccgg cacaggtcgt
     8221 ggtcaaccgc tatatccgcc cgcagtacag ctgtccctgc tgtgagaaag tgttcagcgg
     8281 aaaaatgccg gcacatatcc ttccgaaaag tgctgttgag ccatcggtca tcgcacaggt
     8341 tgtcatcagc aaatataccg accatctgcc cctgtatcgc cagcagcaca ttttctcccg
     8401 aatgggggtg gagctccccg tcagtacaat ggcggatatg gtcggtgttg ctggcgctgc
     8461 actggccccg ctggcaaaat tgttacgcca cgagcttctg acacgggatg ttattcatgc
     8521 agatgaaacg tccctgaggc tccttgatac ccggaaaggc ggtaaatcct gctccggctg
     8581 gctgtgggct tacgtcagcg gagaaagagt aagcgtcaac ggagcaccgt attgacgctt
     8641 atttattggt gagtactacg ttccatggca ggagttcatc aactcggttg gaaggccatt
     8701 ctggcagtac gctcaggata tggcgcagat acgcttccgg atcgataccg ttcagacggc
     8761 aggtgccgat cagcccgtac aacagtgctc cacgctcgcc gccgtgatcg ctgccaaaga
     8821 acataaagtt tttctttccg agacagactg cacgaagcgc tctttccgca gcattattat
     8881 ccgcctccgc cagaccgtca tcactgtaat aacagagggc atcccactga ttcagtacat
     8941 agctgcacgc ttcgcccagt ctggattttt tcgacagcgt accattcttc tccaccatcc
     9001 attcatgcag tgacgtcagt aacactttgc ttcgctgctg cctgacggca agacgctctg
     9061 actccggtaa tccccgtatt tcatcctcga tggcgtacag ttcactgatt cgcttcaggg
     9121 cttcttctgc cgtcgcactt ttgctgctga tgtatacatc gtggattttt cgccgggcat
     9181 gggcccagca cgcaacttct gtcagtgcac caccttcacg ttc
//
LOCUS       DAZFWL020000164         9151 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFWL020000164 DAZFWL020000000
VERSION     DAZFWL020000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.164"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            305..457
                     /gene="hokE"
                     /locus_tag="ACHKNP_RS21550"
                     /old_locus_tag="pgaptmp_004310"
     CDS             305..457
                     /gene="hokE"
                     /locus_tag="ACHKNP_RS21550"
                     /old_locus_tag="pgaptmp_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU4706362.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(579..1199)
                     /gene="entD"
                     /locus_tag="ACHKNP_RS21555"
                     /old_locus_tag="pgaptmp_004311"
     CDS             complement(579..1199)
                     /gene="entD"
                     /locus_tag="ACHKNP_RS21555"
                     /old_locus_tag="pgaptmp_004311"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HGU4706363.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1236..1334)
                     /locus_tag="ACHKNP_RS21560"
                     /old_locus_tag="pgaptmp_004312"
                     /pseudo
     CDS             complement(1236..1334)
                     /locus_tag="ACHKNP_RS21560"
                     /old_locus_tag="pgaptmp_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1374..3614)
                     /gene="fepA"
                     /locus_tag="ACHKNP_RS21565"
                     /old_locus_tag="pgaptmp_004313"
     CDS             complement(1374..3614)
                     /gene="fepA"
                     /locus_tag="ACHKNP_RS21565"
                     /old_locus_tag="pgaptmp_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HGU4706364.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDILVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGMSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            3857..5058
                     /gene="fes"
                     /locus_tag="ACHKNP_RS21570"
                     /old_locus_tag="pgaptmp_004314"
                     /pseudo
     CDS             3857..5058
                     /gene="fes"
                     /locus_tag="ACHKNP_RS21570"
                     /old_locus_tag="pgaptmp_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
     gene            5061..5279
                     /gene="ybdZ"
                     /locus_tag="ACHKNP_RS21575"
                     /old_locus_tag="pgaptmp_004315"
     CDS             5061..5279
                     /gene="ybdZ"
                     /locus_tag="ACHKNP_RS21575"
                     /old_locus_tag="pgaptmp_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HGU4706365.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            5276..>9151
                     /gene="entF"
                     /locus_tag="ACHKNP_RS21580"
                     /old_locus_tag="pgaptmp_004316"
     CDS             5276..>9151
                     /gene="entF"
                     /locus_tag="ACHKNP_RS21580"
                     /old_locus_tag="pgaptmp_004316"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HGU4706366.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLN"
BASE COUNT         1976 a         2432 c         2538 g         2205 t
ORIGIN      
        1 atggtaaggc acctgcttta cactttcgcc cgcggtcagt gatggctgcg ggcgatttcg
       61 ttttaagagc aatgatgagt tttgccgaca gaagtccgaa acaggattca ctgtataaat
      121 aaacagatat tttgttgagg aagggtaaga taacggcggg tgcctgaagc tttccggttt
      181 caggttaact ctgaggtctg gaaagatgaa gccccaggag atatttctat caatcctggg
      241 gctgccactc cgagcctcaa caactcggat ggtagtccct tcttcgcatg gaggcaatat
      301 aaacatgctg acgaaatatg cccttgtggc agtaatagtg ctgtgtttga cggtactggg
      361 atttacgctt ctggtcggag actcgctgtg tgagttcacc gtaaaggaac gtaatattga
      421 gtttaaggct gttctcgctt acgaaccgaa gaagtagccg ttgtgcgggg agcaatcccc
      481 gcatatccgg ttgtcaggtc agagtggtaa ggcacctgct ttacactttc gcccgcggtc
      541 agtgatggct gcgggcgaat cgtaccagat gttgtcaatt aatcgtgttg gcacagagtt
      601 atgactattt tttcttttat ctgccagtgc acagcaaaca tctcattctc acgatgaatg
      661 atgacctgct gtttattcca gctaattatc tgatagtcca gaaaacctgc atcagtttgg
      721 atctcacttg ccttaaatgc gctctctttg gcggaaaatg ccagtgtcag cgccagagaa
      781 aaggctaaac cgcagtctgc gagtcgctcg tgttctgctg gtgtaataat gttgtctgtc
      841 aattcggtcg ctgtttgcgc agaaaaaatt tcttcgatat caacgccaat cggttgacgg
      901 gaaaccacgg ccaatgccgt agccccgcag tggctaatac tgccgtatac ctccgcaggc
      961 cacaccggtt ggcgtagctc gccgattgcg ggcacacatt tatagccata ttcccgcagc
     1021 gcataaattg cagcgatccg cccggctaaa tgttcggctt tgcgcttccg tccagcgtgt
     1081 tgcagttgcg cgtaatgcgg cagccagagt aaatcctgct cacaaaaatt cgccggatcg
     1141 aactcaacaa aatgcagcgt atgtccggca aaggggaggg aggtatgcgt agttttcatg
     1201 tcgaccatat tagagactga tgacaaacgc taaactgcct gatgcgctac gcttatcagg
     1261 cctacatggt cctgcaatat attgaattgg caagattttt gtaggccgga taaggcgttc
     1321 acgccgcatc cggcatgaac gacgcgcact ttgtcaacaa tctgacgtta gcatcagaag
     1381 tgagtgttta cgctcatgta ccacgtacgt cccggctcgt tataggtata cgccccggca
     1441 cccgcgatat agttggcccc tgccagatcg cccgtggtct gggcattacc cgcacgccac
     1501 aaacgtttgt cgaacaggtt gtccacgccg ccggtcagac tgacattctt cgtcacatcc
     1561 caggtcgcgc tcagacccac aatgctgtaa ggactgattt ctttggtttc cgggccaacc
     1621 gctggctgac ctttgtagtt gtattttttc ggctgctgct taccgtacca ggtgaaggtc
     1681 gtttgcatcg acaaatcttc ccgcgcctgc cagctcagcg ttgagttcaa cgtgtactcc
     1741 gggatgatcg acaaacggtc gcccgtggtt ttgttttcac tcttcagcat ataagtgatg
     1801 ttattggtcc acatcaccgt ttcgctaact ggtacgttta acgatccttc cagaccttca
     1861 accactgctt tcggcacgtt atcccactga tagagatcgg tgccgactgc gttttgccct
     1921 acagccacat agcccgcttc aatcttattg cgataatcgt tacggaacca ggtgacgccc
     1981 gccagccagc cgtcgcgttt gaactccaga ccaatctctt tgttgatgct ggtttctgct
     2041 ttcaggtcat cgttaccttg cagatagcag ccgcccgcgc tggcatagca gccctgacct
     2101 ttactgtaga gaatgtagtt cgggttagtc tggtacaggc tcggtgcttt ataagcacgg
     2161 gcaatgccca ttttgagcgt gaagtcatcg cctaaacctt gcgatatgtt cagcgccggg
     2221 ctccagttat tgccgacaat actgtgatga tcgaaacgca gccccggcgt tacgatggtg
     2281 ctgtcagtca gctccatgtt gttttcggca aacagcgaga aaatttctgc ttttgaatac
     2341 gggctacggt cggtggcact cataccatca atagcgccgc cggtgttcgc cccggtcagc
     2401 gcctgggtat tggaactcaa gtccttcatc cgttgctgat tccactccgt acccagcgtc
     2461 agcgtctggt taacgaggat atcaatcggc aggttaactt cgctgtgcag catcacgtca
     2521 tcgagatcga tatcgacgaa atcctgtgtc gctttttcgt taaatttccc ttcggtaccg
     2581 cccgccagac cttccggaat acgcgagtta cgggtgtgtt catactgcac ccagttgctg
     2641 gtggtcacgc cgttatccca gccaccgttc caggtcagcg cgtagttctg gcgatacagg
     2701 cggttggttt catcgccata tttcgagcga gtgtaagcgt cagagttggt gttctgggta
     2761 tcgcccgcat acaggttacc ctggcggctg taacccgctt ccagttccag cgattgcaat
     2821 ggtgcgaagt cccagcgcac cacgccgtta atatctttat tgatcacccc ttcgcgcccg
     2881 gctggtaacg tcgtggcata cgttccggca cgcgcggact gatggccctg gttgatatcc
     2941 cacgcgtcag cctgggtttt gtcgaggttg ccatacaagc ggaagctgaa ttcgtcgccc
     3001 agcggaccgg tcaggctgaa gttggtgcgt ttagtggctc cttcctcttt atgttctggt
     3061 gcattgaaat aagcgtccca ggagccgtgc cactcaccgc tgcctttttt ggtaatgatg
     3121 ttaaccacgc cgcccgccgc gccgttgcca taacgcgcag ctgccggacc acgcagaact
     3181 tcaatacgtt caatcatttc aggtggcacc caggaggtat caccacgggt atcgcgctcg
     3241 ccacgccagc cctgacgcac cgagttacgg ctgcttaccg gcttgccgtc aatcaaaatc
     3301 agcgtgtttt ccggacccat accgcgaata tcaatctgtc ggttattccc acgctgacca
     3361 ctggtggagt taccggtcag gttaacgcct ggcatggtac ggatgatctc cgacacatcg
     3421 cgggcaacgg ggtttttgcg gatttcatct gcggtgatgg tcgaaacgcc aggcgcctgt
     3481 aagttctgct cggcggcggt aacgacaatg gtatcgtcat gtgaaacagg agtatcggtc
     3541 ggctcttgtg cctgcgctac cccataaatc cccagattga ccaacaaggc cagggaatga
     3601 atcttcttgt tcattgtttt attcctgcat ttttgccacg aattgcaact gtcgggcatg
     3661 gtcgtcctca acacgacgca tcccgctacc gcgaaaacct ttgatcctga aagacacgca
     3721 gtgcagttgg ttaataaatg tccgcgcttc ccacggcgcg ccattacgct attgcaaatg
     3781 caaatagtta tcaataatat tatcaatata tttctgcaat caatgaaaaa ttgcacagta
     3841 aacatggggt tatggtgtga cggcgttaaa agtaggaagt gagagctggt ggcagtcgaa
     3901 acatggcccg gaatggcagc gtctgaatga cgaaatgttt gaggtcactt tctggtggcg
     3961 tgatccccaa ggttctgaag aatactcgac gataaagcgc gtatggatct acatcactgg
     4021 tgtgaccgat catcatcaga acagccagcc ccagtcgatg cagcgaattg caggtactga
     4081 tgtctggcag tggacgacac aactcaatgc caactggcgc ggcagctact gctttattcc
     4141 caccgaacgc gatgacattt tttctgcacc atcccccgat cgcctcgaat tacgtgaagg
     4201 ctggcgaaaa ctattacccc aggcaatagc cgatccgctg aatccacaaa gctggaaagg
     4261 cgggcgaggg cacgctgttt ctgcactcga aatgccgcaa gcgcctctgc aaccgggatg
     4321 ggattgtccg caagcgccag aaatagcagc caaagaaatt atctggaaaa gtgaacggtt
     4381 gaaaaattca cgtcgtgtat ggatttttac caccggcgat gcaacagcag aagaacgtcc
     4441 gctggcagtt ttgctcgatg gcgaattttg ggcgcaaagt atgcccgtct ggcctgtgct
     4501 gacttcgctg acccatcgtc agcaacttcc tcccgccgtg tatgtgttga tcgacgctat
     4561 cgacaccacg caccgcgccc acgaactgcc gtgtaatgcg gatttctggc tggcagtaca
     4621 gcaagagtta ttacccctgg tgaaagctat tgcccctttt agcgatcgcg ccgatcgcac
     4681 cgtggtcgcc gggcagagtt ttggtgggct gtccgcgctg tatgccggac tgcactggcc
     4741 tgaacgcttt ggctgtgtat taagccagtc aggatcgtac tggtggccgc atcggggcgg
     4801 gcagcaagag ggcgtgttac ttgaaaagct taaagctggt gaagttagcg ccgaaggtct
     4861 gcgcattgtg ctggaagcgg gtattcgcga gccgatgatt atgcgggcca atcaggcgct
     4921 gtatgcgcaa ttacacccca taaaagaatc cattttctgg cgtcaggttg acggcggaca
     4981 tgatgcgctt tgttggcgcg gtggcttgat gcaggggcta atcgacctct ggcaaccact
     5041 ttccatgaca ggagttgaat atggcattca gtaatccctt cgatgatcca cagggagcgt
     5101 tttacatttt gcgcaatgcg caggggcaat tcagtctgtg gccgcaacaa tgcgccttac
     5161 cggcaggctg ggacattgtg tgtcagccgc agtcacaggc gtcctgccag cagtggctgg
     5221 aagtccactg gcgtactctg acaccggcca atttcaccca gttgcaggag gcacaatgag
     5281 ccagcattta cctttggtcg ccgcacagcc cggcatctgg atggcagaaa aactgtcgga
     5341 tttaccctcc gcctggagcg tggcgcatta cgttgagtta actggagatg ttgatgcgcc
     5401 attactggcc cgcgcggtgg ttgtcggact agcgcaagca gatacgttgc ggatgcgttt
     5461 tacggaagat aacggcgaag tctggcagtg ggtcgatgat gcgctgatat tcgaactgcc
     5521 agaaattatc gacctgcgaa ccaatattga tccgcacggt actgcgcagg cattaatgct
     5581 ggcggatttg caacaagatt tgcgcgtcga tagcggtaaa ccactggtct ttcatcagct
     5641 gatacatgtt gcggataacc gctggtactg gtatcagcgt tatcaccatt tgctggtcga
     5701 tggcttcagt ttcccggcca ttactcgcca gatcgccaat atttactgca catggctgcg
     5761 tggcgaacca acgcctgctt cgccgtttac gcctttcgct gatgtagtgg aagagtacca
     5821 gcaataccgc gaaagcgaag cctggcagcg tgatgcggca ttctgggcag aacagcgtcg
     5881 tcaactgccg ccgcccgcgt cactttctcc ggcaccttta gcggggcgca gcgcttcggc
     5941 agatattctg cgcctgaaac tggaatttac cgacggggaa ttccgccagc tggctacgca
     6001 actttcaggt gtgcagcgta ccgatttagc ccttgcgctg gcagccttgt ggctggggcg
     6061 attgtgcaat cgcatggact atgccgccgg atttatcttt atgcgtcgac tgggctcggc
     6121 ggcgctgacg gctagcggac ccgtgctcaa cgttttaccg ttgggtattc acattgcggc
     6181 gcaagaaacg ctgccggaac tggcaacccg actggcagca caactgaaaa aaatgcgtcg
     6241 tcatcaacgt tacgatgccg aacaaattgt ccgtgacagc gggcgagcgg caggagagga
     6301 accgctgttc ggtccggtac tcaatatcaa ggtatttgat taccaactgg atattcctgg
     6361 tgttcagacg caaacccata ccctggcaac cggtccggtt aatgaccttg aactggccct
     6421 gttcccggat gaacacggtg atttgagtat tgagatcctc gccaataaac agcgttacga
     6481 tgagccaacg ttaatccagc atgctgaacg cctgaaaatg ctgattgccc agttcgccgc
     6541 ggatccggcg ctgttgtgcg gcgatgtcga tattatgctg ccaggtgagt atgcgcagct
     6601 ggcgcagatc aacgccactc aggttgagat tccagaaacc acgctgagcg cgctggtggc
     6661 agaacaagcg gcaaaaacac cggatgctcc ggcgctggca gatgcgcgtt acctgttcag
     6721 ctatcgggaa atgcgcgagc aggtggtggc gctggcgaat ctgctgcgtg agcgcggcgt
     6781 taaaccaggg gacagcgtgg cggtggcact accgcgctcg gtctttttga ccctggcact
     6841 ccatgcaata gttgaagctg gcgcggcctg gctaccgctg gataccggct atccggacga
     6901 tcgcctgaaa atgatgctgg aagatgcgcg tccgtcgctg ttaattacca ccgacgatca
     6961 actgccgcgc tttagcgatg ttcccaattt aacaagcctt tgctataacg ccccgcttac
     7021 cccccagggc agtgcgccgc tgcaactttc acaaccgcac cacacggctt atatcatctt
     7081 tacctctggc tccaccggca ggccgaaagg ggtaatggtc gggcagacgg ctatcgttaa
     7141 ccgcctgtta tggatgcaaa atcactaccc gcttacaggc gaagatgtcg ttgcccaaaa
     7201 aacgccgtgc agttttgatg tctcggtgtg ggagtttttc tggccgttta tcgcaggggc
     7261 aaaactggtg atggctgaac cggaagcgca ccgtgacccg ctcgctatgc agcaattctt
     7321 tgccgaatat ggcgtaacga ccacgcactt tgtgccgtcg atgctggcgg catttgttgc
     7381 atcgctgacg ccgcaaaccg ctcgccagaa ttgcgcgacg ttgaaacagg ttttctgtag
     7441 tggtgaggcc ttaccggctg atttatgccg cgaatggcaa cagttaaccg gcgcgccgtt
     7501 gcataacctg tatggcccga cggaagcggc ggtagatgtc agctggtatc cggcttttgg
     7561 cgaggaactg gcacaggtgc gcggcagcag tgtgccgatt ggttatccgg tatggaatac
     7621 gggtctgcgt attcttgatg cgatgatgca tccggtgccg ccgggtgtgg cgggtgatct
     7681 ctatctcacc ggcatccaac tggcgcaggg gtatcttgga cgacccgatc tgaccgccag
     7741 ccgttttatt gccgatcctt ttgccccagg tgaacggatg tatcgtaccg gagacgtcgc
     7801 ccgctggctg gataacggcg cggtggagta cctcgggcgc agtgacgatc agctaaaaat
     7861 tcgcgggcag cgtatcgaac tgggcgaaat cgatcgcgtg atgcaggcgc tgccggatgt
     7921 cgaacaggca gttacccacg cctgtgtgat taaccaggcg gcagccaccg gtggtgatgc
     7981 gcgtcagttg gtgggctatc tggtgtcgca atcgggactg ccgttggata ccagcgcatt
     8041 acaggcacag cttcgcgaaa cattgccgcc gcatatggtg ccggtcgttc tgctgcaact
     8101 tccgcagttg ccgcttagcg ccaacggcaa gctggatcgc aaagccttac cgttgcctga
     8161 actgaagaca caagcgtcgg gacgtgcacc gaaagcgggc agtgaaacga ttatcgccgc
     8221 ggcattcgcg tcgttgctgg gttgtgacgt gcaggatgcc gatgctgatt tctttgcgct
     8281 gggcggtcat tcgctactgg caatgaaact agcagcgcag ttaagtcgac agtttgctcg
     8341 tcaggtgacg ccggggcagg tgatggtcgc gtcaaccgtc gccaaactgg caacgattat
     8401 tgatggtgaa gaggacagct cccggcgcat gggattcgaa accattctgc cgttgcgtga
     8461 aggtaatggc ccgacgctgt tttgtttcca tccggcgtcc ggttttgcct ggcagtttag
     8521 cgtgctctcg cgttatctcg atccacaatg gtcgattatt ggcattcagt cgccgcgccc
     8581 tcatggaccc atgcagacag cgacaaacct ggatgaagtc tgcgaagcgc atctggcaac
     8641 gttacttgag caacaaccgc acggccctta ttacctgctg gggtattccc ttggcggtac
     8701 gctggcgcag ggtattgcgg cgcgactgcg tgcccgtggc gaacaggtgg catttcttgg
     8761 tttgctggat acctggccgc cagagacgca aaactggcag gaaaaagagg ctaatggtct
     8821 ggacccggaa gtgctggcgg agattaaccg cgaacgcgag gccttcctgg cggcacagca
     8881 gggaagtact tcaacggagt tgtttaccac cattgaaggc aactacgctg atgctgtgcg
     8941 cttgttaacc accgcccata gcgttccgtt tgatggaaaa gcaacgttat ttgttgctga
     9001 gcgcacactt caggaaggta tgagtcccga acgtgcctgg tcgccgtgga ttgcggagct
     9061 ggatatctat cgtcaggatt gtgcgcatgt ggatattatc tctccagggg cgtttgtaaa
     9121 aattgggccg attattcgcg caacgctaaa c
//
LOCUS       DAZFWL020000165         9107 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFWL020000165 DAZFWL020000000
VERSION     DAZFWL020000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.165"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..63)
                     /locus_tag="ACHKNP_RS21585"
                     /old_locus_tag="pgaptmp_004317"
     CDS             complement(<1..63)
                     /locus_tag="ACHKNP_RS21585"
                     /old_locus_tag="pgaptmp_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706367.1"
                     /translation="MSQNLDATAINQIHALISAQG"
     gene            complement(129..491)
                     /gene="yfdP"
                     /locus_tag="ACHKNP_RS21590"
                     /old_locus_tag="pgaptmp_004318"
     CDS             complement(129..491)
                     /gene="yfdP"
                     /locus_tag="ACHKNP_RS21590"
                     /old_locus_tag="pgaptmp_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HGU4706368.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(1158..1832)
                     /gene="ymfK"
                     /locus_tag="ACHKNP_RS21595"
                     /old_locus_tag="pgaptmp_004319"
     CDS             complement(1158..1832)
                     /gene="ymfK"
                     /locus_tag="ACHKNP_RS21595"
                     /old_locus_tag="pgaptmp_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HGU4706369.1"
                     /translation="MKTVHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            1923..2123
                     /gene="ymfT"
                     /locus_tag="ACHKNP_RS21600"
                     /old_locus_tag="pgaptmp_004320"
     CDS             1923..2123
                     /gene="ymfT"
                     /locus_tag="ACHKNP_RS21600"
                     /old_locus_tag="pgaptmp_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HGU4706370.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            2167..2718
                     /gene="ymfL"
                     /locus_tag="ACHKNP_RS21605"
                     /old_locus_tag="pgaptmp_004321"
     CDS             2167..2718
                     /gene="ymfL"
                     /locus_tag="ACHKNP_RS21605"
                     /old_locus_tag="pgaptmp_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HGU4706371.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            2864..3043
                     /locus_tag="ACHKNP_RS21610"
                     /old_locus_tag="pgaptmp_004322"
     CDS             2864..3043
                     /locus_tag="ACHKNP_RS21610"
                     /old_locus_tag="pgaptmp_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4706372.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            2973..3866
                     /locus_tag="ACHKNP_RS21615"
                     /old_locus_tag="pgaptmp_004323"
     CDS             2973..3866
                     /locus_tag="ACHKNP_RS21615"
                     /old_locus_tag="pgaptmp_004323"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4706373.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSAIPGRYTVQAPYKAGAGRG
                     NPEFTKAHNRALAVFLCHEQHYAQIMVGRAGPTSVGPGSLVTGISTPVRLTTNKVVES
                     LGGELLKITKEAAIMATIPTLTQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRTL
                     ECSASFTARNFSVSDYTDCTGRKLPCYQITRDGFAFLAMGFTGKRAAQFKEAYINAFN
                     QMEKQLSKLAVPSDVAHNASVLYSYISSIHQVWLQQLYPMLEKAESPLAVSLYDRIND
                     AAFLARLIHSSLNSSEVRGRK"
     gene            3863..4087
                     /locus_tag="ACHKNP_RS21620"
                     /old_locus_tag="pgaptmp_004324"
     CDS             3863..4087
                     /locus_tag="ACHKNP_RS21620"
                     /old_locus_tag="pgaptmp_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706374.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            4084..4902
                     /locus_tag="ACHKNP_RS21625"
                     /old_locus_tag="pgaptmp_004325"
     CDS             4084..4902
                     /locus_tag="ACHKNP_RS21625"
                     /old_locus_tag="pgaptmp_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4706375.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASADESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARRDDPYFKASYDNVDYSQIPAGFRG"
     gene            4899..5393
                     /gene="yfdN"
                     /locus_tag="ACHKNP_RS21630"
                     /old_locus_tag="pgaptmp_004326"
     CDS             4899..5393
                     /gene="yfdN"
                     /locus_tag="ACHKNP_RS21630"
                     /old_locus_tag="pgaptmp_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4706376.1"
                     /translation="MIMSLLNDVQKFIEAHPGCASGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRQTEVLQESEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            5393..6046
                     /locus_tag="ACHKNP_RS21635"
                     /old_locus_tag="pgaptmp_004327"
     CDS             5393..6046
                     /locus_tag="ACHKNP_RS21635"
                     /old_locus_tag="pgaptmp_004327"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU4706377.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASSMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            6043..6369
                     /locus_tag="ACHKNP_RS21640"
                     /old_locus_tag="pgaptmp_004328"
     CDS             6043..6369
                     /locus_tag="ACHKNP_RS21640"
                     /old_locus_tag="pgaptmp_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HGU4706378.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRATHWLH
                     ERGLKV"
     gene            6366..6755
                     /locus_tag="ACHKNP_RS21645"
                     /old_locus_tag="pgaptmp_004329"
     CDS             6366..6755
                     /locus_tag="ACHKNP_RS21645"
                     /old_locus_tag="pgaptmp_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4706379.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISEAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKPAGAAA"
     gene            6775..7584
                     /locus_tag="ACHKNP_RS21650"
                     /old_locus_tag="pgaptmp_004330"
     CDS             6775..7584
                     /locus_tag="ACHKNP_RS21650"
                     /old_locus_tag="pgaptmp_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU4706380.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGGEQKKRPKYWLSNK
                     QTCELIEQLFTEGGIPPLEKNQPVSVINGGNNQGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSASEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            7592..8581
                     /locus_tag="ACHKNP_RS21655"
                     /old_locus_tag="pgaptmp_004331"
     CDS             7592..8581
                     /locus_tag="ACHKNP_RS21655"
                     /old_locus_tag="pgaptmp_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4706381.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKVLRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MATKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            8599..8940
                     /locus_tag="ACHKNP_RS21660"
                     /old_locus_tag="pgaptmp_004332"
     CDS             8599..8940
                     /locus_tag="ACHKNP_RS21660"
                     /old_locus_tag="pgaptmp_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4706382.1"
                     /translation="MRDMYEVLDRWGAWAAADNSGVDWQPVAAGFKGLLPHGKKTRQQ
                     CDDDEGIMIDSCVARLRKYKPEEYELVIAHFVIGISLRTIAKKQKCSDGTIRKELQTA
                     LGFLDGVLSML"
BASE COUNT         2258 a         2127 c         2450 g         2272 t
ORIGIN      
        1 accctgagca gaaataaggg catggatttg attaattgcg gttgcgtcta agttctgaga
       61 cataataagt cctcactata taaagatatt cagtgatgag ataaataatc agttaattaa
      121 gaacgatatt aatgacctgc tgcgcggagt tttccgtcag gttcaccggc aagagtcagt
      181 aattgtccct ggtcttcctg cagaatagtc agtcgaccac cgcgattgac atacatcggc
      241 gtttcggtgg tgtcttcttc ggaaattttc ccgcggttag tcgggcgaac atatgagagt
      301 ttgtgtttga ttttcacacg gttctcatca aacggttcga tttccaggtt gagcgagacc
      361 ttacctttgg ttttcgtgtt catcacaccg gaagcgactt cactgagaac tgcgccgatt
      421 ttggtttcaa atacgccgcc gtccagctcc ccgataaatg cctgcacatc agtactgcgt
      481 tcgctagcca ttttgctgct cctcatcata tcgaccctgc aaggtcggtt ggtttctcca
      541 caaaacagag aagaacacct gcggtggcag ccgcccggct ggattgggtt atgagcccgt
      601 cgtccggtga tgctcttctc tgttttgtaa aaagagcggt accagccgga agcaagtgta
      661 caaactggta ccgccaaagc agtggctgtt gtggtggggt tgtcactcag gcgtatggtc
      721 aacctgacaa tccggtgtcc tcaacgggga aagagtaacc ccgccatact taccgccgcg
      781 ccatttcgcg gattaccaca acgctgagag cacttagcaa gttacggcac cacactttgt
      841 cgcggttcca taaatgccct catcgttgca ccctggtctc ttcccaggcg tcaaaccgga
      901 tcgccgcgct ggttaggcgt cttatcagca tcatcattga cttgcacatt ccggctacct
      961 ggtttttttg cccgagcaag gagtggattg tcccctttaa cgtccccaga ccgctaacga
     1021 cgcatgtgcc atacgccgtg ttacaaccaa attttgttta atcttgcctg cggcatgttt
     1081 cttttagata cattatgtat ctcaagggta cattgtcaag tataaaaaaa cctgccgaag
     1141 caggttataa atattgatta ggcctttatt ttgtatcttc ttggttttcc tgagaaaatt
     1201 actgtaccaa ttatagagca attaccgtta atcttaatgt aaggctcagg ccagtttggg
     1261 tttaatgctt tgagataacg ctgtgttcca tcttctatca atcgcttgaa ggtggtttcg
     1321 cctgtatcgt gcatcaatgc aataacgtcg tcaccgtggc aggcagggac ttcgggatca
     1381 acaaaaatca tgtctcccgg gcggtactca tcaatcattg aatcaccaat cacccgcaag
     1441 atataagtca tttcgccaca gggtacaggg cagggataag tttctgctgt gctcaaatca
     1501 acctcagaat agccaacttc tttccatgct ccggcctgta cccatgatat gacagggact
     1561 aacgttattt gtttgttagt gattgaaaca tcaggttttt ttgtgatgtt cgtggtctgg
     1621 tgttcttgat caagccatcc gacaggcagg tcgaaacatt tttcgatgtg ccgtgccatg
     1681 ctgtcaccga tatttttagt agcaccatct cccataaacc tgctggtctg ggttggctcg
     1741 cgatcaatca tggtggcaaa ggaagaattc ccgccaacac catctctcag ttttctggcg
     1801 ttagaccgcc ggatgtcatg gactgttttc ataaagaaat taaaaccttt gtaccgataa
     1861 ggtacaagta tcttgaaggt tcatattaat catgtaatat gtatattgga ggtacatatt
     1921 gtatgaaagc gtattgggac tctttaacca aagaacagca gggcgagttg gccggaaaag
     1981 ttggctcaac accaggctac ttacggctgg ttttcaatgg ttataaaaaa gccagttttg
     2041 tgctggctaa aaaacttgag caatgcacgt caggtgcaat tacgaaatct gacttaagac
     2101 cggatatcta tccgaaagat taacagaaca ccttcaattt ttaaccacag aacgatgagg
     2161 ctaaccgtgg gtaagtatca ctggaaagta gaaaaacagc ctgagtggta cgtgaaagct
     2221 gtcagaaaaa ctatcgcagc gttgccgggt ggttacgctg aagcagctga ctggctggat
     2281 gtaacagaga acgcattatt taaccgcctt cgtgccgatg gcgatcagat tttcccgctg
     2341 ggatgggcaa tggttttaca gcgcgcggct ggcactcact acattgcgga tgctgtcgca
     2401 cagtctgctg gtggggtgtt cgtatcgctt cctgaaattg aggaagtaga gaacgccgat
     2461 ataaaccagc gcctgctgga agtcatcgaa cagatcggga attactcaaa gcagattcgt
     2521 tcggcaatcg aagatggggt cgtggagcca cacgagcaga cagcaattaa tgatgagttg
     2581 tatctgtcaa tttcgaagct ccaggagcat gcagcactgg tctacaaaat cttttgcgct
     2641 ccagaaaaga gtgacgcccg cgagtgtgca gctccgggcg tcgtggcgtt ttgtgtctgt
     2701 ggagaaacta acgcatgaac agtttaacgg caaataaccg tttgtcgcaa cagctggtgg
     2761 tcagtgtcgc tgaacacctg ttgttacggc atgaatgcag attaccaaat cacctggctg
     2821 taagtaacca cagagaactt tacctgactg tggggggcga gttgtgcagg aacttaaccg
     2881 ctggtttcgt gacggaagag ggctttatgt ccatgttatt cgttgggagc cagaaacaca
     2941 gcgcgttatc tatcttcgca aagactaccc gcatgagtgc tttagtcctt tgtggaaatt
     3001 caggcgtgat tttgttgagt gtgaaggacc accagcacat tgattctgcc attccgggac
     3061 gttacactgt tcaggcacct tataaagcgg gtgccgggcg tggaaacccg gaattcacca
     3121 aagcgcacaa ccgcgctctt gcggtttttt tgtgtcatga gcagcattac gcccaaatta
     3181 tggtggggcg tgcagggcca acttcggttg ggccgggttc tttggtgacc ggtatttcca
     3241 cccctgtacg tctcaccacc aataaggtcg tggaaagcct tggtggtgag ttattaaaaa
     3301 tcaccaaaga ggctgccatc atggctacga tcccaaccct cactcaaccc gaaattgcca
     3361 tcgttgatgg tcaggctgtt acttcatccc tggctgttgc caacttcttc tccaaacgtc
     3421 atgacgatgt tctgaaaaag atccgcacgc ttgaatgctc cgcatcattc actgcccgca
     3481 atttttcggt gagtgattac accgattgca caggccgcaa actaccttgc tatcaaataa
     3541 cccgcgacgg ctttgcgttt cttgctatgg gtttcacggg taaacgtgct gcccagttca
     3601 aagaggcata catcaatgcc tttaaccaga tggagaaaca gctttcaaag ctcgctgtac
     3661 cgagtgacgt tgcacataac gccagcgttc tctattccta catttcatca attcatcagg
     3721 tctggttgca gcagctttat cccatgctgg aaaaagctga atcaccgctg gctgtaagtc
     3781 tgtatgaccg aattaacgat gcggcatttc ttgcccgtct tattcattcg tcgctgaact
     3841 cttcagaggt aagggggcgc aaatgatccg aaatattttc aaacggttta ccaatcaaac
     3901 tttccgttgt cctcgcccgg gtcagtggta caccacgcct gcagggcatg ttctacgtgt
     3961 tagcctggtt gaccgtgaat gtcagaaggt gatttgtgaa ccgctgggtc gtaattaccg
     4021 cgtcagtatg ccgctcatag cctttcgctc cggaaaaaac atgaagcatc tcggaggtgc
     4081 agcatgagta tggagctgat ggttaaagcg atgaaaattc gagtgggtaa tccattgcga
     4141 aaactggttc tgatcaagct ggctgataat gccagcgatc agggtgagtg ctggcccagc
     4201 taccagcata ttgctgacca gtgcgagatt agcaaacgtt ctgtgatgaa tcatattgcg
     4261 gccctttgtg agtccgggct ggtaaaaaaa gtcacccgga aaggtgaaaa aggtaactca
     4321 agtaatatct atctccttca tctggatggt gcaggagatt cactaggggg tagtgcaaat
     4381 aattcactat ctggtgcagc aaattcacca ggtagtgcag gagttgcacc agggggtagt
     4441 gcaggagatt cacccagaac cagtcactct tttgaaccag tcaaagaacc agtcaatgaa
     4501 ccaatagctg ttggtgcatc tgctgatgag tctgtgcgag ttcgttcaaa ccgaccggaa
     4561 tactctccgg agtttgagca ggcatggctg gcatacccca aacgtgctgg tggcaattca
     4621 aaatcagcag ccttcaaagc ctggaaagcc cgtttgaacg agggggtaaa ccccgaaacc
     4681 atgctggaag gtgtgaaacg ctacgcgggc tgggtatctg cgatgggcaa tagcggcaca
     4741 caatttgtga aacaggctgt cacgttcttt ggtccggatc gtcatttcga agaatcctgg
     4801 gaagttcctg cggtatctgc agccagacgc gatgacccgt acttcaaagc cagttacgac
     4861 aacgtggact acagccagat cccggcagga ttcagggggt gatcatgagt ctgttaaatg
     4921 acgttcagaa attcattgaa gcccatccgg ggtgtgcttc cggagacatt gcggatgctt
     4981 ttgcaggtta ctcacgacag cgcgttctgc agtcagcaag caagttacgt cagagtgggc
     5041 gtgtggctca ccgttgtgaa ggggatactc gcagacattt cccgcgccag actgaggttt
     5101 tgcaggaatc ggaaccgcaa ccagttcgtg aaaccagacc tgtgcgcaat ttctatgtcg
     5161 gcactaacga cccccgggtg attttgtgcc tgacccgcca ggctgaagaa ctggagtcca
     5221 ggggcttata ccgtcgtgct gcaacggtgt ggatggcggc attccgtgaa agccactccc
     5281 agccagaacg aaacaatttt ctggcgcgtc gtgagcggtg cttacggaaa agcagcaagc
     5341 gcgctgcatc gggtgaagag tggtatctgt cagggaatta cgtgggggct taatgagtaa
     5401 taaatattgc caggcgctgg tggaactgcg gaacaaacca gcccatgaac tgaaggaagt
     5461 gggcgatcag tggcgcacgc cggacaacat tttctgggga attaacaccc tgtttggccc
     5521 gtttgttctg gatctgttta ctgacggtga taacgccaaa tgtgccgcgt attacacggc
     5581 ggaagataac gcgctggcgc atgactggtc agaacgtctt gcggagctta aaggtgctgc
     5641 ctttggtaat cccccataca gccgcgccag tcagcatgag gggcaataca tcaccggcat
     5701 gcgttacatc atgaaacatg ccagttccat gcgtgataag ggcgggcgct atgttttcct
     5761 gatcaaagct gccaccagcg aagtgtggtg gccggaagat gcggaccata ttgcttttat
     5821 tcgcgggcgt attggttttg aactgcctgc ctggtttatc ccgaaggacg agaagcaggt
     5881 gccgacagga gcttttttcg ctggtgctat tgctgttttt gacaagacct ggaagggacc
     5941 ggcaatcagc tacatcgggc gcgatgaact tgaggcatgt ggtgaggcgt ttctggcgca
     6001 ggttcgccag caggcggaaa aactggtcag ggagatggcg gcatgacgac gttaactcaa
     6061 tgccagcagc aggtgctgga tatgctgatt tcttatcaga aagaacgtgg cttcccgcca
     6121 accaatcagg aggtggcaac catgctggga taccgttcgg tgaatgcagc ggtggagcat
     6181 cttcgcgcac tggagaaaaa aggcgtcatc acgataaagc gtggtgtggc ccgggggatc
     6241 acgcttcata ccgcggtgaa ggacgacgac agcgaggcgg tcgggattat ccgctcactg
     6301 cttgccggtg aggaaaacgc aaggctgcgt gcaactcact ggttacatga gagaggcctg
     6361 aaagtatgaa gctgatcctg cctttcccgc ccagcgtgaa cacgtactgg cgacacccca
     6421 acaaaggggc atttgctggt aagagcctga taagcgaggc ggggcgaaaa tttcagagcg
     6481 cggcgtgcgc agcaatagtt gagcagttac gtcgtctgcc gaaaccaacg tcggcacctg
     6541 cttcagtgga gatcgtgttg tttcctccgg ataaccggat ccgcgatctg gacaactata
     6601 acaaggcgct gtttgacgcc ctgacccacg cgggtgtgtg ggaagacgac agccaggtga
     6661 aaagaatgct ggtggagtgg ggaccggtta tcccgaaagg gaaggtcgag atcactatca
     6721 gtaagtatga gaaaccggcg ggtgcagccg cctgattaag aggagaaacg aagtatgaat
     6781 aatctgatgg tcattgatgg tattgaagtt cgtcgtgatg cttatgggcg ttacagcctg
     6841 aacgatctgc acagggcagc cgggggagaa caaaaaaaac gcccgaaata ctggctctcc
     6901 aataagcaaa cctgtgaatt gattgaacaa cttttcaccg agggtggaat tccgcctctg
     6961 gaaaagaatc aaccagttag cgttattaat ggcggaaata accaggggac gtatgtctgc
     7021 aaagaactgg tgtatgccta tgcaatgtgg atcagcccgt cattccatct gaaggtgatc
     7081 cgtactttcg atatggtaac cagcgcatcg gaaaaattat ccgggcaggc tgctgacaag
     7141 atgcaggctg gcgtgattct gctggacttt atgcgccggg agttaaacct gtctaactct
     7201 tcagtgcttg gtgcctgtca gaaactccag gaggctgttg gcttaccgaa tctggcaccg
     7261 cgctatgcca ttgatgctcc tgctgacgcg cctgatggct caagccgtcc cacgctgtca
     7321 ctgagtgcac tgctgaagca gtatggtatc cgcctgacag ctaatcaggc atatcaccag
     7381 atggtgaagc tggggatcgt cgagcagcgc gaacgataca gccgtaccgc gattaacaac
     7441 atcaaaaaat tctggtcgct gacagcgaaa ggctgcatgt tcggcaagaa catcaccagt
     7501 cccgcaaatc cgcgcgagac gcagccgcat ttcttcgaat cccgattccc tgagctgtta
     7561 aagctgctcg ataccgttca ttgaggtgac cgtgagagca ctactgaccc ctgaaattgc
     7621 cccgcgtatg gggatcgtat tgttcaggcc aggttcagag ctgatgcccc tgtttatgca
     7681 ggggcgtgtc ctgctggagc ctgagccgga acgttattca tctttcgcca gtggtgccgt
     7741 tccggcggca tcacaaccgc tggcggatga tcctgccgtt cgggccgtgt tccgcaatga
     7801 ggcagtgatc cgtcgtgctg gtggcgtgga atgtcttgaa agctggttac ttcgtgaaaa
     7861 aggctgccag tggcctcatt ccgactggca cagcgagaac atgaccacaa tgcgacacgc
     7921 gccgggcgca atccgtctgt gctggcactg cgataaccag ctgcgcgatc agttcacgga
     7981 acggctggaa tcaatggcaa cggataactg tgcccgctgg gtgttgtctg tcgtgcgtcg
     8041 ggatctcggt tttgatgaca gtcacgttgt gacaatgccg gaactgtgct ggtggctgat
     8101 tcgtaatgat ctggcggatg ccttaccgga aagtgcagcc cgtaaggtac tgagattacc
     8161 gaagcctgtt gtgccgtctg tcacccggga aagtgacctt gtgccttcgg ttcctgccac
     8221 cagcatcatc caggataaag cgaaaaaggt gctggcgctg aaagtggatc cggagtcgcc
     8281 ggagtctttt atgttacgcc caaaacgtcg ccgctgggtt aatgaaaagt acacgcgatg
     8341 ggttaagacg cagccgtgcg catgttgtgg aaaacctgct gatgatcccc accacctgat
     8401 aggtcacggt cagggtggaa tggctacaaa agcgcatgac ctctttgtgt tgcctttgtg
     8461 cagaaagcat cacgacgagc tgcatgcgga taccgtggca tttgaagaga agtatggctc
     8521 ccagctggag ctgatatttc gttttatcga tcgtgcactg gcaattggcg tgctggcctg
     8581 attttgtgga gaaagttgat gcgtgatatg tatgaagtat tggaccgctg gggtgcatgg
     8641 gctgcagcag ataacagtgg tgtggactgg cagcctgttg ctgcagggtt taaaggttta
     8701 ctacctcatg gaaagaaaac acgccaacaa tgcgatgatg atgaaggaat catgattgac
     8761 agctgtgttg cgcgattgcg gaaatataag cccgaagagt atgagttggt tattgctcat
     8821 tttgttatcg gcatctcact aagaactatt gcaaagaagc agaagtgttc tgatgggaca
     8881 ataagaaaag agttgcaaac agctcttggt tttctggatg gtgtgctttc aatgttgtaa
     8941 tattaggggg aattaccccc ctttttttct ctgttgcttt aataaaattt taatattttg
     9001 tctgattatg atgaggcaat gtaataaaag aaataccgtt agtattgcaa gccatacgca
     9061 aaataagcat gcatataaat tagttgaaag tccaatagtg aattgtg
//
LOCUS       DAZFWL020000166         9107 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFWL020000166 DAZFWL020000000
VERSION     DAZFWL020000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.166"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            275..1333
                     /gene="citC"
                     /locus_tag="ACHKNP_RS21665"
                     /old_locus_tag="pgaptmp_004333"
     CDS             275..1333
                     /gene="citC"
                     /locus_tag="ACHKNP_RS21665"
                     /old_locus_tag="pgaptmp_004333"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HGU4706383.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1348..1644
                     /gene="citD"
                     /locus_tag="ACHKNP_RS21670"
                     /old_locus_tag="pgaptmp_004334"
     CDS             1348..1644
                     /gene="citD"
                     /locus_tag="ACHKNP_RS21670"
                     /old_locus_tag="pgaptmp_004334"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HGU4706384.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1641..2549
                     /gene="citE"
                     /locus_tag="ACHKNP_RS21675"
                     /old_locus_tag="pgaptmp_004335"
     CDS             1641..2549
                     /gene="citE"
                     /locus_tag="ACHKNP_RS21675"
                     /old_locus_tag="pgaptmp_004335"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HGU4706385.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2560..4092
                     /gene="citF"
                     /locus_tag="ACHKNP_RS21680"
                     /old_locus_tag="pgaptmp_004336"
     CDS             2560..4092
                     /gene="citF"
                     /locus_tag="ACHKNP_RS21680"
                     /old_locus_tag="pgaptmp_004336"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HGU4706386.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLATFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4096..4647
                     /gene="citX"
                     /locus_tag="ACHKNP_RS21685"
                     /old_locus_tag="pgaptmp_004337"
     CDS             4096..4647
                     /gene="citX"
                     /locus_tag="ACHKNP_RS21685"
                     /old_locus_tag="pgaptmp_004337"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HGU4706387.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4622..5500
                     /gene="citG"
                     /locus_tag="ACHKNP_RS21690"
                     /old_locus_tag="pgaptmp_004338"
     CDS             4622..5500
                     /gene="citG"
                     /locus_tag="ACHKNP_RS21690"
                     /old_locus_tag="pgaptmp_004338"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HGU4706388.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5551..7014
                     /gene="citT"
                     /locus_tag="ACHKNP_RS21695"
                     /old_locus_tag="pgaptmp_004339"
     CDS             5551..7014
                     /gene="citT"
                     /locus_tag="ACHKNP_RS21695"
                     /old_locus_tag="pgaptmp_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HGU4706389.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7128..7934
                     /gene="rna"
                     /locus_tag="ACHKNP_RS21700"
                     /old_locus_tag="pgaptmp_004340"
     CDS             7128..7934
                     /gene="rna"
                     /locus_tag="ACHKNP_RS21700"
                     /old_locus_tag="pgaptmp_004340"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HGU4706390.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8164..8574
                     /gene="rnk"
                     /locus_tag="ACHKNP_RS21705"
                     /old_locus_tag="pgaptmp_004341"
     CDS             8164..8574
                     /gene="rnk"
                     /locus_tag="ACHKNP_RS21705"
                     /old_locus_tag="pgaptmp_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HGU4706391.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8712..8801
                     /gene="yldA"
                     /locus_tag="ACHKNP_RS21710"
                     /old_locus_tag="pgaptmp_004342"
     CDS             8712..8801
                     /gene="yldA"
                     /locus_tag="ACHKNP_RS21710"
                     /old_locus_tag="pgaptmp_004342"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HGU4706392.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8805..>9107)
                     /locus_tag="ACHKNP_RS21715"
                     /old_locus_tag="pgaptmp_004343"
     CDS             complement(8805..>9107)
                     /locus_tag="ACHKNP_RS21715"
                     /old_locus_tag="pgaptmp_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706393.1"
                     /translation="FIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEE
                     IVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2123 a         2457 c         2321 g         2206 t
ORIGIN      
        1 atcaattaaa tcacttaaac ctgctggtca tttcgtaatt acattaatta aaaaacttaa
       61 aaccattaaa taaataaaaa accactaact ctatgtgaaa taaatcaaaa tttcacgccg
      121 aaatactcct taggatgtat agcgaaagga gaaaaagata tacctcgatc accccccttt
      181 ctcccaagtg aaaataaaag gttatcagtt tgcaacattg aacaacattc gttgcaaatc
      241 gataacaaca tgcaccttca ggatactatt tattatgttc ggcaatgata ttttcacccg
      301 cgtaaaacgt tcagaaaata aaaaaatggc ggaaatcgcc caattcctgc atgaaaatga
      361 tttgagcgtt gacaccacag tcgaagtatt tattaccgta acccgcgatg aaaagcttat
      421 cgcgtgcggt ggaattgccg gaaatattat taaatgcgtt gctatcagtg aatccgttcg
      481 cggtgaagga ctggcgctga cattagccac tgaactgata aacctcgcct atgagcggca
      541 cagcacgcat ctgtttattt ataccaaaac cgaatacgag gcgctgttcc gccagtgcgg
      601 tttttccacg ctgaccagcg tacccggcgt gatggtgctg atggaaaaca gcgccacgcg
      661 gctgaaacgc tatgccgaat cgctgaaaaa atttcgtcat ccagggaaca agattggctg
      721 cattgtgatg aacgccaatc ccttcacgaa tggtcaccgt tatctgattc aacaagctgc
      781 ggcacagtgc gactggttgc atctgttttt agtcaaagaa gattcttcac gcttccccta
      841 tgaagaccgg ttggatctgg tgttaaaagg caccgccgat attccacgcc tgactgtgca
      901 tcgcggctcc gaatacatca tctcccgcgc tacgttccct tgctacttca ttaaagaaca
      961 gagcgtcatt aaccattgtt acaccgaaat tgatctgaag attttccgtc agtacctcgc
     1021 tcccgcgctg ggtgtaactc accgctttgt cggtactgaa cccttttgtc gcgttaccgc
     1081 ccagtacaac caggatatgc gctactggct ggaaacgccg actatctccg caccgcccat
     1141 cgaactggtt gaaattgagc ggctgcgtta ccaggagatg ccgatatccg cttcccgggt
     1201 acgtcaactg ctggcgaaaa acgatctcac ggctatcgcg ccgctggtcc ctgcagtcac
     1261 gctgcattat ttgcagaacc tgcttgagca ctcccgccag gacgcggcag ctcgtcaaaa
     1321 gacccccgca tgagaaacag gtgaaaaatg aaaataaacc agcccgccgt tgcaggcacc
     1381 cttgagtctg gggatgtgat gatacgcatc gccccactcg atacgcagga tatcgacctg
     1441 caaatcaata gcagcgttga gaaacagttt ggcgatgcaa ttcgcaccac cattctggac
     1501 gttctcgccc gctacaacgt gcgcggcgta cagctgaatg tcgatgacaa aggcgcactg
     1561 gactgcattt tacgtgcacg actggaagcc ctgctggcac gcgccagcgg tatcccggct
     1621 ctgccatggg aggattgcca atgatttccg cttcgctgca acaacgtaaa actcgcaccc
     1681 gccgcagcat gttgtttgtg cctggtgcca atgccgcgat ggtcagcaac tccttcatct
     1741 acccggctga tgccctgatg tttgacctcg aagactccgt ggcattacgt gaaaaagaca
     1801 ccgcccgccg catggtttat cacgcgctgc aacatccgct gtatcgcgat attgaaacca
     1861 ttgtgcgtgt caacgcgctg gattccgaat ggggtgttaa cgacctggaa gccgtcgttc
     1921 gcggtggtgc ggacgttgtg cgtctgccga aaaccgatac cgctcaggat gttctggata
     1981 ttgaaaaaga gatcctgcgt atcgaaaaag cctgtggtcg tgaacccggc agcaccggcc
     2041 tgctggcggc gattgaatct ccgctgggca ttacccgcgc agtggaaatc gctcacgctt
     2101 ccgagcgttt gatcggtatc gccctcggtg cagaagacta tgtgcgcaac ctgcgtacag
     2161 aacgctcccc ggaaggaact gaactgctgt tcgcacgctg ttccattttg caggccgcgc
     2221 gctctgcggg tattcaggcg ttcgataccg tctattccga cgctaacaac gaagccggat
     2281 tcctgcaaga agccgcccac atcaaacagc tgggctttga cggcaaatcg ctgatcaacc
     2341 cgcgtcagat tgatctgttg cacaacctct acgcaccaac ccagaaagaa gtggatcacg
     2401 cccgccgcgt cgtagaagcc gctgaagccg ccgctcgcga aggcctcggc gtggtttccc
     2461 tgaacggcaa gatggtggac ggtccggtta tcgatcgcgc ccgtctggtg ctctctcgtg
     2521 cagaactttc cggcatccgc gaagaataag gcaatcaaaa tgacgcagaa aattgaacaa
     2581 tctcaacgac aagaacgggt agcggcctgg aatcgtcgcg ctgaatgcga tcttgccact
     2641 ttccagaact cgccaaagca aacctaccag gctgaaaaag cgcgcgatcg caaactgtgc
     2701 gccaacctgg aagaagcgat tcgtcgctct ggtttacagg acggcatgac ggtttccttc
     2761 catcacgctt tccgtggcgg tgacctgacc gtcaatatgg tgatggacgt catcgcgaag
     2821 atgggcttta aaaacctgac cctggcgtcc agctccctga gtgattgcca tgcgccgttg
     2881 gtagaacaca ttcgccaggg cgtggttacc cgcatttata cctccggcct gcgcggtcca
     2941 ctggcggaag agatctcccg tggtctgctg gcagaaccgg tgcaaatcca ctctcacggc
     3001 ggtcgcgtgc atctggtaca gagcggcgaa ctgaatatcg acgtggcttt cctcggcgtc
     3061 ccgtcctgcg atgaattcgg taatgccaac ggctacaccg gtaaagcctg ctgcggctcc
     3121 ctcggctatg caatggttga tgccgacaac gcaaagcagg tcgtgatgct taccgaagaa
     3181 ctgctgcctt atccgcataa tccggcaagc attgagcaag atcaggttga tttgatcgtc
     3241 aaagttgacc gcgttggcga tgctgcaaaa atcggcgctg gcgcgacccg tatgaccact
     3301 aacccgcgcg aactgcttat tgcccgtagc gctgcggatg tgattgtcaa ctcaggctac
     3361 ttcaaagaag gtttctccat gcaaaccggc accggcggcg catcgctggc ggtaacccgt
     3421 ttcctggaag acaaaatgcg tagccgcgat attcgcgccg acttcgccct tggcggtatt
     3481 accgcgacga tggttgacct gcacgaaaaa ggtctgatcc gcaaactgct ggatgtgcag
     3541 agctttgaca gccatgctgc gcaatcgctg gcccgtaacc ccaatcacat cgaaatcagc
     3601 gccaaccagt acgctaactg gggttcgaaa ggcgcatcgg ttgatcgtct cgacgtggtg
     3661 gtactgagcg cgctggaaat tgacacccag ttcaacgtta acgtgctgac cggctctgac
     3721 ggcgtactgc gtggtgcttc cggtggtcac tgcgataccg cgattgcctc tgcgctttcc
     3781 atcatcgtcg cgccgctggt acgcggtcgt attccgactc tggtggataa cgtgctgacc
     3841 tgcatcaccc cgggctccag tgtcgatatt ctggtcacag accacggtat cgcagttaac
     3901 ccggcacgtc cggaactggc agaacgtctg caggaagcgg gcattaaagt ggtttccatt
     3961 gagtggctgc gcgaacgtgc gcgtctgctg accggtgaac cacagccgat tgaattcacc
     4021 gaccgcgtcg ttgccgttgt gcgttaccgc gatggctcgg tgatcgatgt tgtgcatcag
     4081 gtgaaggaat aagccatgca cctgcttcct gaactcgcca gccaccatgc ggtgtcaatt
     4141 cccgagctgc tcgtcagccg ggatgaaagg caagcacggc aacacgtctg gctcaagcgc
     4201 catcctgttc cactggtctc ctttaccgtg gttgcgcctg ggccgattaa agacagcgag
     4261 gtcacacgcc gaatttttaa tcatggcgtg acagccttgc gtgccttagc cgcaaaacag
     4321 ggctggcaaa ttcaggagca ggctgcactg gtttccgcca gcgggccgga gggcatgttg
     4381 agcattgccg ccccggctcg cgacctcaag ctcgccacca ttgagcttga acatagtcat
     4441 cctctcgggc ggttatggga tatcgatgtc ctgacgcccg aaggcgaaat tctctcccgc
     4501 cgcgactatt cactgccgcc tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc
     4561 gcgcgtggaa aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac
     4621 gatgtcgatg cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga
     4681 gtacgccctg ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg
     4741 cctcgtggat cgcattaact gcggtgcgca caaagatatg gcgctggaag atttccaccg
     4801 cagcgcgctg gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc
     4861 agaaatggca ccggaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg
     4921 tgatatgttc cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg
     4981 gctgctatgt gcggcaattg gccgtttgct tcagctcaac cagccggtaa cgccaacaac
     5041 cgtttgttct acggcggcaa gtttctgccg tggtctgacc gatcgcgaac tgcgtaccaa
     5101 taattcacaa ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg
     5161 cggtgaagcc gaagcgggtt atccactggt gatcaaccac gccttgccgc attacctcac
     5221 tctgctggat caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat
     5281 ggcgatcaac ggcgatacca acgttgcttc gcgcggtggc gaggggggcc tgcgctggct
     5341 acagcgcgag gcgcaaacat tattgcaaaa agggggcatt cgaacccccg ccgatctcga
     5401 ttatctccgg cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc
     5461 cgacctgctg atccttacct ggtttttagc acagatttaa ttatttaagc acttgataaa
     5521 tttgaaaata ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa
     5581 ctattggccc cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg
     5641 ccaccacagg catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc
     5701 gagccaattc cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt
     5761 aattacctgc tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag
     5821 gcgctgaaat ggggtctggc tggtttttcc agcaccacgg tatggctggt atttggcgca
     5881 tttatttttg cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg
     5941 gtgaaattca tgggcaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt
     6001 ctgctggcac cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc
     6061 attaaaaacc tgccgccgtt gtttaaatca ttccccaacg atccgtccgc gcgtcgtatt
     6121 ggcggctatt tgatgtggat gatggtcatt agtaccagtc tgagttcgtc catgtttgtc
     6181 accggtgcgg caccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag
     6241 attagctggt tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt
     6301 gcgccgtggc tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca
     6361 acctgggcgg gtgatgaact gaaaaccatg ggtgcgctga cacgcagaga gtggacactg
     6421 attggccttg tattgctcag cttaggtttg tgggtttttg gcagcgaagt cattaatgct
     6481 actgcagttg gtctgctggc agtttcgcta atgctggccc tgcacgttgt gccttggaaa
     6541 gatattaccc gctataacag cgcatggaac acgctggtca acctagcaac tctggttgtg
     6601 atggctaacg gcctgactcg ttctggcttt attgactggt tcgccggtac catgagtacg
     6661 cacctggaag gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt
     6721 gcacactacc tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg
     6781 gccgtcggta aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg
     6841 tctatcggta tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac
     6901 ggctgtggct atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt
     6961 tacatctcta tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt
     7021 cgccaaacgc tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta
     7081 tgatttaata gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct
     7141 ggcgtaacgc cgcgttgctc gcggtttctc tgcttccctt ctcttctgcc aacgccttag
     7201 cgttgcaggc aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa
     7261 ccggattttg ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc
     7321 aaaccgaaac gaccaacaaa gctgattttc tgaccgtaca tggtctgtgg ccaggattgc
     7381 ctaaatcggt tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta
     7441 ctcgcccaat cccgaatcta ccagaagcgc acgccagccg gatgtgttca tcgccggaaa
     7501 ccggattatc actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac
     7561 gttcctgcct ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg
     7621 acgcatactt cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa
     7681 aattccttgc ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg
     7741 ccaaaagctg gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg
     7801 cgtatttgac tgaaattcag atctcgatca aagctgacgc aatcaacgct ccgctttctg
     7861 caaactcatt cttgccacaa ccacacccag gcaactgtgg caaaaccttt gtgattgata
     7921 aagtgggtta ttaatcatct gatttacaaa acacgctaat tcgtcttcca taaaccgtga
     7981 cgattaagaa ttatacataa tgcgctgcat tgaggaattc cctgaatgtg acgcaaatca
     8041 cttcaaagag tgagcaacag tcagtatcat catgtgagtt aaaccctcgc cgcctgacgg
     8101 tgagggtttt cttttgggta tgtttatctg cgacactcgc agtaccgacg acatggagta
     8161 aaaatgtcca gaccaactat catcattaac gacctggatg ccgaacgcat cgatattctg
     8221 ctggagcaac ccgcctatgc tggtttgcca atcgccgacg cgttaaacgc agagttggat
     8281 cgcgcccaaa tgtgttcgcc agaagagatg ccacacgacg tggtgacaat gaacagccgg
     8341 gttaaattcc gcaatcttag cgatggcgaa gtgcgtgtgc gcacgctggt gtatccggca
     8401 aaaatgaccg atagcaatac tcagctttcc gttatggctc cggtaggtgc cgcactgctg
     8461 gggctgcgcg ttggcgattc cattcactgg gaacttccgg gcggcgttgc aacccacctt
     8521 gaagtgctgg aactcgaata ccagccagaa gctgctggcg actacctgct ttaatcccat
     8581 ctgcacaagc gataactcgc tttgcccaga ggatgcactc gcatcctctt tacgcaaaat
     8641 cagcaatttt tcctggttct cactgttcac ttttgctgca aaattattcc gtttcgtttg
     8701 tgggaataac catggcagaa gcattttaca tcctgatagg atttctgatt atggcggcga
     8761 ttatcgtcat ggctgtcctt tacctcgaaa accactcctg acgatcatat tgttcccccc
     8821 ggcatcgcat tcaccagacc tgaaaccgcc ttctgcgccg cctctgcgct ttgtgcaccg
     8881 ggtaccagaa tcaccttacg gcactcctct tcacgttttt cgaaaatctc atatccccgg
     8941 gcggcctctt caaacggcat atagtgggta acaatttctt ctggtttcag taatcctttc
     9001 tcaattaacg gtaataattc tcccagccat gcgtgaacgt gggtctgtcc cattttaaac
     9061 gtcaaccctt tatcaaaggc gtcgccaaac aggaaaccgt gaataaa
//
LOCUS       DAZFWL020000167         8933 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFWL020000167 DAZFWL020000000
VERSION     DAZFWL020000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.167"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1054
                     /locus_tag="ACHKNP_RS21720"
                     /old_locus_tag="pgaptmp_004344"
     CDS             <1..1054
                     /locus_tag="ACHKNP_RS21720"
                     /old_locus_tag="pgaptmp_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU4706394.1"
                     /translation="IHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDN
                     ILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTK
                     ILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGI
                     EVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFC
                     VMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELN
                     SAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKAL
                     REAKSEGGNKVIIHHI"
     gene            1235..2725
                     /gene="dgcJ"
                     /locus_tag="ACHKNP_RS21725"
                     /old_locus_tag="pgaptmp_004345"
     CDS             1235..2725
                     /gene="dgcJ"
                     /locus_tag="ACHKNP_RS21725"
                     /old_locus_tag="pgaptmp_004345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HGU4706395.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2768..3271
                     /gene="yeaK"
                     /locus_tag="ACHKNP_RS21730"
                     /old_locus_tag="pgaptmp_004346"
     CDS             2768..3271
                     /gene="yeaK"
                     /locus_tag="ACHKNP_RS21730"
                     /old_locus_tag="pgaptmp_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HGU4706396.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3272..3376)
                     /gene="yoaI"
                     /locus_tag="ACHKNP_RS21735"
                     /old_locus_tag="pgaptmp_004347"
     CDS             complement(3272..3376)
                     /gene="yoaI"
                     /locus_tag="ACHKNP_RS21735"
                     /old_locus_tag="pgaptmp_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HGU4706397.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3546..3992
                     /gene="yeaL"
                     /locus_tag="ACHKNP_RS21740"
                     /old_locus_tag="pgaptmp_004348"
     CDS             3546..3992
                     /gene="yeaL"
                     /locus_tag="ACHKNP_RS21740"
                     /old_locus_tag="pgaptmp_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HGU4706398.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3949..4770)
                     /gene="nimR"
                     /locus_tag="ACHKNP_RS21745"
                     /old_locus_tag="pgaptmp_004349"
     CDS             complement(3949..4770)
                     /gene="nimR"
                     /locus_tag="ACHKNP_RS21745"
                     /old_locus_tag="pgaptmp_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HGU4706399.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4867..6048
                     /gene="nimT"
                     /locus_tag="ACHKNP_RS21750"
                     /old_locus_tag="pgaptmp_004350"
     CDS             4867..6048
                     /gene="nimT"
                     /locus_tag="ACHKNP_RS21750"
                     /old_locus_tag="pgaptmp_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HGU4706400.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRTLHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6103..6450
                     /gene="yeaO"
                     /locus_tag="ACHKNP_RS21755"
                     /old_locus_tag="pgaptmp_004351"
     CDS             6103..6450
                     /gene="yeaO"
                     /locus_tag="ACHKNP_RS21755"
                     /old_locus_tag="pgaptmp_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HGU4706401.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6472..6726)
                     /gene="yoaF"
                     /locus_tag="ACHKNP_RS21760"
                     /old_locus_tag="pgaptmp_004352"
     CDS             complement(6472..6726)
                     /gene="yoaF"
                     /locus_tag="ACHKNP_RS21760"
                     /old_locus_tag="pgaptmp_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HGU4706402.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6909..7934
                     /gene="dgcP"
                     /locus_tag="ACHKNP_RS21765"
                     /old_locus_tag="pgaptmp_004353"
     CDS             6909..7934
                     /gene="dgcP"
                     /locus_tag="ACHKNP_RS21765"
                     /old_locus_tag="pgaptmp_004353"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HGU4706403.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            7967..8065
                     /gene="yoaK"
                     /locus_tag="ACHKNP_RS21770"
                     /old_locus_tag="pgaptmp_004354"
     CDS             7967..8065
                     /gene="yoaK"
                     /locus_tag="ACHKNP_RS21770"
                     /old_locus_tag="pgaptmp_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HGU4706404.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8068..8142
                     /gene="yoaJ"
                     /locus_tag="ACHKNP_RS21775"
                     /old_locus_tag="pgaptmp_004355"
     CDS             8068..8142
                     /gene="yoaJ"
                     /locus_tag="ACHKNP_RS21775"
                     /old_locus_tag="pgaptmp_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HGU4706405.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8201..8449)
                     /gene="yeaQ"
                     /locus_tag="ACHKNP_RS21780"
                     /old_locus_tag="pgaptmp_004356"
     CDS             complement(8201..8449)
                     /gene="yeaQ"
                     /locus_tag="ACHKNP_RS21780"
                     /old_locus_tag="pgaptmp_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU4706406.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8677..>8933)
                     /locus_tag="ACHKNP_RS21785"
                     /old_locus_tag="pgaptmp_004357"
     CDS             complement(8677..>8933)
                     /locus_tag="ACHKNP_RS21785"
                     /old_locus_tag="pgaptmp_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU4706407.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSQFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         2207 a         2165 c         2107 g         2454 t
ORIGIN      
        1 tatccataaa ccaattaacg gcagtacact gatccagaca aagtcgaatg atgtttctat
       61 tttctatatt ttccgccaac tcagttttat ttgtttaacc tcgctggcac tcttttgtta
      121 tggaaaagac aacatccttg acaacaataa gaaaaaaacg ggaatcctgt tgctggcgct
      181 gattcctttt ttagtttttc cccttctggc acacaatctg agcagttata acgctgacta
      241 ttctttgtat gtcgtcgact actgtccgga caaccatact gcgacctggg gaatcaacta
      301 tacaaaaata ttggtttgtc tgtgggcatt tttactgttc tttattatca tgcgtacacg
      361 attagccagc gaactatggc cgttaatagc attattatgt ctggcatcgc tatgctgcaa
      421 cttacttcta ctgactctgg atgagtataa ttacaccatc tggtatatca gtcgcgggat
      481 tgaagtttcc agtaaactgt ttgttgtgtc ttttctgatt tataacattt ttcaggaact
      541 gcaactctcc agcaaactgg cagttcatga tgtgctgacc aatatttata atcggcgcta
      601 ctttttcaac agcgtagagt cattattgtc gcgacctgtt gttaaggact tctgtgtcat
      661 gctggttgat attaatcagt tcaaacgcat caatgcccaa tggggacatc gtgtgggtga
      721 taaagtgctg gtttcaattg tcgatattat ccagcaaagc atccgccctg atgatatttt
      781 agcgcgactg gagggtgagg tgtttggctt gctatttacc gaactcaata gtgcccaggc
      841 aaaaatcatt gcggaacgta tgcgtaaaaa tgtcgaactc ctgaccggct ttagtaacag
      901 atatgatgtt cctgaacaaa tgaccatcag tattggcacg gttttttcaa cgggtgacac
      961 gcgtaatatc tcgcttgtca tgacggaagc agataaagcc ttacgcgaag cgaaaagcga
     1021 ggggggcaac aaagtgatta ttcatcatat ttaagtgcaa aaatattcag agccatgctt
     1081 tttgcgtggc ttttgcatac aatttattac tattacccct aaatttcccc tccccagcgg
     1141 cgcagagatg agtataatta gcgcccctgt gccaggccgc aatcgaactt tatctggttt
     1201 tctcgtttca ctaaccgaag gagtgccatt tatcatgaaa ttgcaccata gaatgctccg
     1261 gcattttatc gccgcaagtg tcattgtgct gacatcttcc ttccttattt ttgaacttgt
     1321 cgccagcgac agagcaatga gtgcctatct gcgctatatc gtgcagaaag cagactcctc
     1381 ctttctttat gataagtatc agaatcagag tattgccgcg catgtgatgc gcgctctcgc
     1441 tgctgagcag tcggaagtgt cgccagaaca gcggcgcgcc atctgcgagg cttttgagtc
     1501 tgccaataat acccatggct taaacctgac tgcccataaa tacccgggct tacgcggcac
     1561 actacaaacc gcatccactg actgcgacac aattgtggaa gctgcagcac tattacccgc
     1621 ttttgatcag gcagtggaag gcaaccgcca ccaggatgat tacggttcag gtcttgggat
     1681 ggccgaagag aaatttcact attatctcga tctcaatgac cgctatgtct atttttatga
     1741 gccggttaat gttgaatact ttgcgatgaa taactggtcc ttcctgcagt caggaagtat
     1801 tggcatcgat cgcaaagata ttgaaaaggt atttaccggg cgtaccgtat tgtcgagcat
     1861 ttaccaggat cagcgtacta aacagaacgt gatgagtttg ctgacgccgg tatatgtcgc
     1921 agggcagcta aaagggattg tgctgctgga tattaacaaa aacaatttgc ggaatatctt
     1981 ttacactcat gaccgccctc tcctctggcg ttttctcaat gtcacgctaa ccgataccga
     2041 ttcggggcgc gacattatca tcaaccagag cgaagataat ctgttccagt acgtcagtta
     2101 cgtccatgac ttaccgggcg gcattcgtgt ctcgttatcc attgatattc tttactttat
     2161 cacgtcttcg tggaaaagcg ttctgttctg gattttgacg gcgttaattt tgctgaatat
     2221 ggtccggatg cacttccgtt tataccaaaa tgtgtcgcga gaaaatatta gtgatgcgat
     2281 gactggactg tataatcgca aaattttaac ccctgaactg gagcagcggt tgcagaaact
     2341 ggtgcaatcc ggttcttcgg tgatgtttat tgcaattgac atggacaagt taaagcaaat
     2401 aaatgacacc ctcggtcatc aggaggggga tttagcgatt acgttattag ctcaggcgat
     2461 taaacaatcg attcgtaaaa gtgattatgc catccgactc ggtggcgatg aattctgcat
     2521 cattcttgtc gattcgacgc cgcaaattgc agcacaactg cctgaacgta tcgaaaaacg
     2581 tctgcaacat atcgcgccgc agaaagaaat cggcttctct tccggtattt acgcgatgaa
     2641 agaaaacgat acgttacatg atgcgtataa agcttccgat gagcgtttat atgtcaataa
     2701 gcagaacaaa aacagccgtt catgataacc ttctgtggtt gtttgcttgt aatctcagga
     2761 gcgtgaaatg actgaaatgg ctaaaggaag cgtgacgcat cagcggttaa tcgctttatt
     2821 atcacaagaa ggtgctaact tccgcgtcgt tacccatgaa gcggtaggta aatgtgaagc
     2881 ggtgtcagaa attcgtggca ccgctctagg ccagggtgct aaggcactgg tatgtaaagt
     2941 caaaggcaat ggcgtaaatc aacatgttct ggcgatcctc gctgccgatc aacaggccga
     3001 tctgagccaa ctcgccagcc atataggcgg attacgcgct tcgttggcca gcccggcgga
     3061 agtcgatgaa ctcacaggct gtgtcttcgg cgcgatcccc cctttcagct tccatccaaa
     3121 actcaaactg gttgccgacc ctctactctt cgaacgattt gatgaaatcg ccttcaatgc
     3181 agggatgctg gataaatccg ttattttgaa aaccacggat tatttgcgca ttgcgcaacc
     3241 agaactgctc aatttccgcc gcactgcgta actagcctgt ccgttcaata agcagaacgg
     3301 acaaaaccag cacaacagca atggcaaaaa acgatgacgt gataatcagt gtctcgacga
     3361 acatttgatc gttcatagca tgcccccggg agatagctgt tttcacgtta ttgttagcgt
     3421 gcacaaatgg cagtttgatg acagttcgcc tgatttttta tgcaaaaaac gtaaagattt
     3481 tctacttcct tcctgcagca agcgtaaagt aagcaggctt attatttttt ggcaaggaaa
     3541 ccacgatgtt tgatgtcact ttgctgatcc tgctcgggtt agctgcgctg ggctttatca
     3601 gtcataacac cactgtcgcc gtttcaattc tggtgttgat cattgtccgc gtcacaccgt
     3661 taagcacctt tttcccgtgg attgaaaaac aggggctaag tatcgggata atcattctga
     3721 cgattggcgt tatggccccc atcgccagcg ggacgctacc gccctcaacc ttgattcact
     3781 cgtttctgaa ctggaaatcg ctggtggcaa ttgcagtagg cgttattgtc tcctggctgg
     3841 gtgggcgcgg cgtgacctta atgggcagcc agccgcaact ggtcgccggg ctgctggtag
     3901 gcactgtttt aggcgtggcg ctgtttcgcg gcgtaccggt cggcccgctt attgccgcgg
     3961 gtctggtttc gctgattgtg gggaaacagt agttaatccc gcaatatatc ggcctggcgt
     4021 ttgccccagg cctttcttaa acatagtaat aaaggccgtt gttgaatcat accccagcgt
     4081 atgagcaact ttctgtacag tatcgccttt cactaatccc tgaagtgcca taatcagctg
     4141 taactgctga cgccactggc gaaaactcaa ccccgtctct tttactatca gtcgcgcaag
     4201 attgcgttca ctcatcgcaa aaaagccagc ccattgccct aatgaccccc actcgacggg
     4261 ccctttcgcc atcatctcca ccatcgtgcg gattttagga tgagaagaaa cgggcaggtg
     4321 cagctgttgt tgtggctgct gagggagttc atcaaacagg acctgaatta atctgcgggt
     4381 catcggctca gcacgctgag tcgtcgtcct gtttgccagg gttaaaatca attcccggca
     4441 taagggcgat attttcaatg tgcaacatgt tgtcggcatt gtcactgcgc tgggttcgat
     4501 aaacagaaaa cagagttcag cgttagctgt cacttgattg ctatgttcca ccccgcccgg
     4561 tatccaaacc gcatattgcg gcggcaccat ccacaaagca ttttccaccg tacaggtaat
     4621 tgcaccatgt aatgctagta tcagctgtcc tttgcggtgc tgatgtacag gactggttaa
     4681 ctcatccgtt ccggcgtgaa tacaaaatgc gaccgcggct tcatggtgtc ggtcgggttc
     4741 atagccattg agattcaacc tgtgcatcat tttgtccgaa tttagcgata atttgtcatt
     4801 ttagcttgat tcaacataac aataaaaacg gtaaggtaca gcctcgtttg taacaatgag
     4861 aagcatatga cctgttcaac ttcattaagc ggcaaaaaca ggattgtcct tatcgctggc
     4921 attctgatga ttgccactac attacgcgtc accttcaccg gcgcagcacc gttactcgat
     4981 acgattcgtt ccgcttactc gctgacgaca gcgcaaaccg gcttattaac caccctgcca
     5041 ttattggcct ttgcgctaat ctcacctttg gctgccccgg tggcgcgacg ttttggtatg
     5101 gaacgtagcc tgtttgccgc gttacttttg atctgtgcag gtatcgcaat tcgttctctc
     5161 ccttcgcctt acttattatt tggcggtaca gcggtcattg gcggtgggat tgcattaggc
     5221 aatgtcttac tgccaggatt aattaaacgc gatttccctc attccgtcgc cagacttacc
     5281 ggcgcatatt ccctgacaat gggagctgca gcggcactgg gatcggctat ggtcgtgccg
     5341 ctggctttga acggttttgg ctggcaaggc gcgttgttca tgctgatgtg ttttcctctg
     5401 ctggctcttt ttttatggct gccacagtgg cgaagccaac aacatgcaaa tttgagtacc
     5461 tcgcgcacct tacatactcg gggtatctgg cgttcgccgc ttgcctggca ggtcacattg
     5521 tttcttggga tcaactcact ggtctattac gtgattattg gctggcttcc ggcgatcctc
     5581 atcagtcgcg gctatagcga agcacaggcg ggttcactgc atggtttgct gcaactagcc
     5641 acagcagctc ccggtttgct gatccctctt ttcttacatc atgtgaaaga tcagcgtggt
     5701 attgcagcgt tcgttgcctt gatgtgcgca gtgggcgctg ttgggctctg ctttatgcca
     5761 gcgcacgcga tcacctggac tctgcttttc ggttttggtt ccggcgcaac aatgatactg
     5821 gggttgacgt tcattggtct tcgggcaagt tctgcgcatc aggcggcggc actctcgggg
     5881 atggcacaat ccgtcgggta tttgctggca gcctgtgggc cgccgctgat gggtaaaata
     5941 cacgatgcta acggtgactg gtccgtacca ctgatgggtg ttgccatact ttcactactg
     6001 atggcgattt tcggactttg cgccgggaga gacaaagaaa ttcgctaata tccggtgcta
     6061 tagtgacgtg acaaatcatg cgtgaaaggg agaacaaaca cgatgaatat tcagtgcaaa
     6121 cgcgtttatg atccggctga acagagcgat ggttatcgcg tactggtcga ccgcctctgg
     6181 ccgcgcggta tcaaaaaaac cgatttagcc cttgatgagt gggataaaga aatcacgccg
     6241 tcaacggaac tgcgcaaagc ctttcacggc gaagtcgtcg attttgcaac ctttcgcgag
     6301 caatatcttg cagaactggc gcaacacgag caagaaggaa agcggctggc ggacatcgcc
     6361 aaaaaacagc cgctgaccct gctctacgca gcaaaaaaca ccacgcagaa ccatgcgctg
     6421 gtgctggccg actggctacg tagcttgtga ttttagtaca gcatccggcg gttatttttc
     6481 accagccgga tggtcccgtc gccacaatgc ccattcatca atcgtttcac cgcccggtaa
     6541 tttgcaattg ttgctgaccc cttgcgctgt ctgcactgga atgagcgtcc cgcccttctg
     6601 ctggcaatag accgacgccg gatttgccat accaatctgc ggcggtttag gtgcttctgg
     6661 ctgagaagag gttgaacaac cagccaggac cagcaagcaa ggcaaagcaa aactgataat
     6721 tttcatttat tgatctcaca catttatcta cgcttagagt atcgcggtat cgttttgttt
     6781 tgcagcacta tttttattac attcactcaa aacatattac gtcttgtttc atctttgttg
     6841 atgatgtttt atcatgcctg caaagattaa ataatcagca tttacccgcc gtatcctgga
     6901 gttgtttcgt gtcagatcag attatcgccc gcgtctcgca atcccttgcc aaagaacagt
     6961 cactagaaag tctggtccga cagcttctgg agatgctgga aatggtcact gatatggaat
     7021 caacctacct gaccaaagtg gatgtcgaag cgcgcctgca gcatattatg tttgcccgta
     7081 acagccagaa aatgcacatc ccggagaatt ttaccgtctc gtgggattac tcgttatgca
     7141 aacgcgctat tgatgaaaac tgctttttca gcgatgaagt ccctgaccgt tggggcgact
     7201 gtattgcggc acgcaatctt ggaatcacca catttctgag tacaccaatt cacttaccgg
     7261 atggatcatt ctatggcacg ctttgcgccg ccagcagtga gaagcgccag tggagtgaac
     7321 gcgcggaaca ggttttacag ttattcgccg gactgattgc acaatatatt caaaaagagg
     7381 cgttggttga acagctgcgc gaagccaatg ccgcgctgat tgcgcaatcg tataccgact
     7441 cgttaaccgg gctaccgaat cggcgggcga tttttgaaaa tctgacgacg ctgttttctc
     7501 tcgcccggca tcttaaccat aagataatga tcgcgtttat cgatctggat aacttcaaat
     7561 taatcaatga tcgttttggt cataatagtg gcgatctgtt tctcattcag gttggcgagc
     7621 gccttaatac gctccagcaa aatggcgaag ttattggtcg tctcggcggt gatgagtttt
     7681 tggttgtttc actaaataac gagaatgcgg atatttcgtc gctgcgagaa cgtattcaac
     7741 agcaaatacg tggagaatat cacttaggtg atgttgattt gtattatcct ggtgccagtc
     7801 ttggcattgt agaagtcgat cctgaaacga ccgatgcaga cagtgccctg catgctgccg
     7861 atatcgcgat gtatcaggag aaaaaacaca aacagaaaac accttttgtc gcgcattcag
     7921 cgccacattc ctgaggcgta ttcacatcct tttgattggt gataacatgc gaatcggtat
     7981 tatttttccg gttgtaatct ttattacagc ggtcgtattt ttagcatggt tttttattgg
     8041 cggctatgct gccccgggag cataaagatg aaaaaaacaa cgattattat gatgggtgtg
     8101 gcgattattg tcgtactcgg cactgagctg ggatggtggt aacgtcacct ctaaaaaata
     8161 gcaaaggctg cctgtgtgca gcctttgtgc aatttaagcg ttaactttta atcttcctgt
     8221 agataaatag cacgacaatc gcaccaataa cggcaaccac gaagctgcca aaattgaagc
     8281 catcgacttt accaaagcca aacagcgtgc tgatccatcc gccaactacg gcaccgacta
     8341 tccccagcag gatagtcata aagaatccac caccatcttt acctggcatg atccacttcg
     8401 ccagaatacc ggcaataagc ccaaaaataa tccatgacag aatgcccatt gtttcctcac
     8461 ttatctgttt tgcattagcg ggttagtcgc tgataaaaag catagcacaa catcgggagg
     8521 gcaagatttg tgacgagcat cacggaggat ttttttgcgg atggcacaga aattgcgcca
     8581 tcaacgatca gtgataatta ccaaccacaa acatcatgtt cgttttccgt gtcttgacat
     8641 cctccacgcc ctgaatgacg aggatccctg ctacgttcag gctgtcgcct gaatcatttc
     8701 ggtgggttcc tgcttcaacg ggcggcctga ctgcaccatc tctccacagg caagaacggc
     8761 gtgccccgcc gctaaaatgt tacgagcgcc gtttacatcg gcgttcgctg tatatccaca
     8821 tacctggcat ctgaattgac tttgtgacag gcgattttct ttcgctgtat gaccacagca
     8881 cgcgcaacgc tggcttgtgt acgctggcgg aacagcaagc acctgaccgc cac
//
LOCUS       DAZFWL020000168         8837 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFWL020000168 DAZFWL020000000
VERSION     DAZFWL020000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.168"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..158)
                     /locus_tag="ACHKNP_RS21790"
                     /old_locus_tag="pgaptmp_004358"
     CDS             complement(<1..158)
                     /locus_tag="ACHKNP_RS21790"
                     /old_locus_tag="pgaptmp_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HGU4706408.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTI"
     gene            402..659
                     /gene="ynaJ"
                     /locus_tag="ACHKNP_RS21795"
                     /old_locus_tag="pgaptmp_004359"
     CDS             402..659
                     /gene="ynaJ"
                     /locus_tag="ACHKNP_RS21795"
                     /old_locus_tag="pgaptmp_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HGU4706409.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(709..1659)
                     /gene="uspE"
                     /locus_tag="ACHKNP_RS21800"
                     /old_locus_tag="pgaptmp_004360"
     CDS             complement(709..1659)
                     /gene="uspE"
                     /locus_tag="ACHKNP_RS21800"
                     /old_locus_tag="pgaptmp_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HGU4706410.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1811..2563)
                     /gene="fnr"
                     /locus_tag="ACHKNP_RS21805"
                     /old_locus_tag="pgaptmp_004361"
     CDS             complement(1811..2563)
                     /gene="fnr"
                     /locus_tag="ACHKNP_RS21805"
                     /old_locus_tag="pgaptmp_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HGU4706411.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2758..3273)
                     /gene="ogt"
                     /locus_tag="ACHKNP_RS21810"
                     /old_locus_tag="pgaptmp_004362"
     CDS             complement(2758..3273)
                     /gene="ogt"
                     /locus_tag="ACHKNP_RS21810"
                     /old_locus_tag="pgaptmp_004362"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGU4706412.1"
                     /translation="MLRLLEEKIATPLGPLWVMCDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3284..4810)
                     /gene="abgT"
                     /locus_tag="ACHKNP_RS21815"
                     /old_locus_tag="pgaptmp_004363"
     CDS             complement(3284..4810)
                     /gene="abgT"
                     /locus_tag="ACHKNP_RS21815"
                     /old_locus_tag="pgaptmp_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HGU4706413.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(4847..6292)
                     /gene="abgB"
                     /locus_tag="ACHKNP_RS21820"
                     /old_locus_tag="pgaptmp_004364"
     CDS             complement(4847..6292)
                     /gene="abgB"
                     /locus_tag="ACHKNP_RS21820"
                     /old_locus_tag="pgaptmp_004364"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HGU4706414.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(6292..7602)
                     /gene="abgA"
                     /locus_tag="ACHKNP_RS21825"
                     /old_locus_tag="pgaptmp_004365"
     CDS             complement(6292..7602)
                     /gene="abgA"
                     /locus_tag="ACHKNP_RS21825"
                     /old_locus_tag="pgaptmp_004365"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HGU4706415.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQHHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            7778..8686
                     /gene="abgR"
                     /locus_tag="ACHKNP_RS21830"
                     /old_locus_tag="pgaptmp_004366"
     CDS             7778..8686
                     /gene="abgR"
                     /locus_tag="ACHKNP_RS21830"
                     /old_locus_tag="pgaptmp_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4706416.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
BASE COUNT         2254 a         2217 c         2192 g         2174 t
ORIGIN      
        1 attatggtgt agattaaaaa ttgcaccgca tggaataaaa aaccttttct tttacgattt
       61 ccgcggcgaa accaaaagct catcagaatc aatgctgcgc agctaccgaa aataatgacc
      121 agattaagcg cattatttgt aaacagttca gcgatcattg ttttatcagg ctcctccaga
      181 taattgtcgt catgccggaa acccctggcg gggctatttt accgcgacaa ttcattcaga
      241 tcatcaatag tcagggaagg aagtagcaac attagctaac taatcaatgc gttgattgta
      301 aatccagcta agaggtgagg ttttcagagc agacaacggt gaaatgtcat ggtattgtta
      361 cgtttaggta acaagaaatt tgtctgcaca aggattacat catgattatg gcgaaactga
      421 agtcagcgaa agggaagaaa tttctctttg gtttgttggc ggttttcatt attgcggcgt
      481 cggttgtgac tcgcgcgact atcggcggcg ttatagaaca gtacaatatt ccgctgtctg
      541 agtggacgac atcaatgtat gtgattcagt catcgatgat ttttgtttat agcctggtct
      601 ttactgtgtt gctggcaatc ccgttgggaa tttatttcct tggcggcgaa gagcagtaag
      661 taaaaaatag gcccgataac tcgggccttg tcagttattg aagagtcgtt aatcgtcttc
      721 ttcgtcatcc agttcaacgg gtgtctgata ctggtcaggt ttaatgacca acagatcgca
      781 gcgaagatga tcaatcacct gttccgccgt gttgccgagg aatgctgctg aaataccggt
      841 gcgtcctacc gtgcccagaa ccacaatccc cgcctgtaag tgctccgcca aatcaggaat
      901 cacctcttct ggcagacctt tttctacgtg cgtcatgttt tcattaatgc cgaatttctg
      961 ccgcagagct ttcattgcca gcaaatgttg cccacgaatg gcatcgttat aaacgctcgg
     1021 atcaaattcc ggcagttcaa tcgcgatatt aattggcgtt accggataag cgccaaccag
     1081 atgaacttcg gtatggttga cttgttctgc cagttcgatc gtctctttga ccagtttttc
     1141 attgagcgca ttatgatacg gctcttcact ggcgagattc accgccacca gcgccttgcc
     1201 tccttccggc cacggctggt ctttcaccat ccacaccggg cttgggcatt tgcgtaacag
     1261 atgccagtcc gttggcgtaa aaatcaccgc ttccagacgg tcatgttggt gcgccatttt
     1321 tagcaccaaa tcgtgcccgc cgctgatcac ttcctgaatg atggcttcga agggacggtt
     1381 atgccagacc actttaattt caatgggaac gccagcattg agataatatt ttgcctgctc
     1441 gtggatccag gctgtacgct ggctgatgac gccctgacgc atagcggtac gttcgtccgg
     1501 ggagagcagg gtggtcattt cgtatgagaa gtcatagatc ggcaaaaagg ctttaatttt
     1561 gccaccaatc cgttgatgta aataaacagc tcgccgcaat gctggttggt cgtcctggtt
     1621 aggatcgata acaacgagca tgttctgata catagccata cagggtctcc ttacaacaac
     1681 tgtcaacgca gtttgtaatt aaaagattaa cccatatctg gtgaatgaaa cagtgatgaa
     1741 ccttctgcca gatcaataaa tcagaaaaat ttaatgatat gacagaagga tagtgagtta
     1801 tgcggaaaaa tcaggcaacg ttacgcgtat gaccagcaag ctgggccagc gcatcgttat
     1861 tttcgatggt gatgtattta cctttgactg ccagcatacc gcttttctgg aagcgaccca
     1921 gcagacggct gatggtttct accgtcaggc ccagatagtt accgatatcg ccacgagtca
     1981 tcgtcaggcg gaattcacga ggggagaagc cgcgttgggc aaaacgacgg gacaggttgt
     2041 agatgaatgc agccagacgt tcctcggcat ttttcttcga caacagcaag atcatgtcct
     2101 gatcgccttt gatttcaccg ctcatcagac gcatcatctg ctgacgcaga ttcggcattt
     2161 taccggacaa atcgtccagc gtttcgaacg ggatttcaca taccatcgag gtttccagcg
     2221 cctgcgcgaa gctcgggtga tggccgctgc cgatggcgtc aaatcccacc aggtcgccag
     2281 ctaaatggaa accagtgatt tgctcgtcgc cttgctcagt gatggtataa cttttaatcg
     2341 taccggagcg gatggcataa agcgatttaa gttcatcacc agccttaaac agcgtctggc
     2401 ctttctgaat aggcttcttc cgctcaatga tattatcaag ctgatcaagc tcatgttcgt
     2461 tgagtgtgaa cgggatgcaa agctggctga tgctgcaatc ctggcaatgg atagcacaac
     2521 cgccagactg aatgcgccgt ataattcgct tttccgggat cataggtctg ctcaagccgt
     2581 aattgatatt tgtcaatttt aacatctttt tagggagcaa gtaagtctaa gcaaacctta
     2641 acagcagaga attccgatat tagatgtaaa tatatgtcta tctatttgaa aacccttaag
     2701 ttgttaaggg taactttaca taaaagtgtg aacaagctgg cacaaattgt ttaatgttta
     2761 cagcaaaaga taaccttcgt ggcgcaataa ccactctttt cgctgaactc cgcctgcata
     2821 tccggtcatg gtgccgtttc gaccaataac ccgatggcaa ggtacgacga tgctgatggg
     2881 attcgatcca tttgccgcac ctacggcacg cgccgcgcca gggcggccca attgttcagc
     2941 cagttggccg taatgcatta cctgcccgca ggggatagtg cgtagagttt tccagacttc
     3001 gcgctgaaat ggcgtccccc ctgtggcagt gggaagcgta tcaataatgc taagattacc
     3061 ggcaaaataa tcacgaagct tgtcgcttaa accgcctggg ttggtggcag aaatgcgctc
     3121 atagccttct ttacgataat ggatgtccag cagctgcacc atgcgttcgc tgtactcttc
     3181 ccattcaacc gcccgcaggc gaaattgctc atcgcacatc acccacagtg gacccagtgg
     3241 cgtggcaatt ttttcttcaa gtaatctcag catccgttct ctcttaagac aaacgtgggt
     3301 aaatccccgg acctatcggc agaccaacaa gataccacgc cagcaacatc agcagccata
     3361 ccaccaaaaa gataagcgga tagggcaaga ctaacgaata gtaagtaccc agtttcgcgt
     3421 ctggtttgta gcgttgcagg aatccaagaa acagtggaac aaaaggagat accggcgcta
     3481 aaggcaatac ggatgagtcg gcaatacgaa agaggatttg cgcaaatgcc gggtgaaagc
     3541 caagtagcat aaacattggt acgaaaatgg gggccagaat cgaccagatt gcggaaccgc
     3601 tggcaataaa catgcataag aaagaggaaa gcaacgccag accgacaaac gccgggatgc
     3661 cgctaagccc tgaactttca agtatatcgg tcagccccac ggcgatgaat ttccccatgt
     3721 tgctccagtt aaacatggcg acaaactggg cgaggggaaa aaccatcacg ataaatcccg
     3781 ccatctcttt catcggttca atcattaaat gcggtaaatc cgcctgacgt cgaattgtgc
     3841 gggtagcgat gccataagcc agcgaaacca caaagaaaaa aagaatgatt agtggcacga
     3901 tacctttaat aaagggtgat ggcatcacgg tgtgattaat cggatcgcgc aatatcccgt
     3961 tttccgggat caccatcagc gcgatcgcag caataaaaag tagcgatacg acacctgcta
     4021 tgcgtaaacc aaaacgctga ctttcggtca atgtctgcag tttctcatcg ctgtttccct
     4081 gccattgacc taaccgtggc tcgatgattt tgtcggttat caagccgcca acaatcgtca
     4141 gtacgactac ggagctggcc ataaaatacc agttatcaat tacactgacg tgcatttgcg
     4201 gattgaacgc agccgccgct tccgtgctga tccccgacag caacacgtcg gttgtgacaa
     4261 tcagtaaatt agccgtaaag ccgcaaccta cgcctgcaat ggcagccagt aaacctgcaa
     4321 ctggatgcct gcccaccgcc agaaaaatca gcgcacccat cggtggcatg atcactaacg
     4381 ccgcatcgga agaaatgtgg ctgaaaaaag caataaacag caccatataa ctggcgtagc
     4441 gggcattaac atgcgatgcc attttaacca ttagcgctgg cagtaagccg acgcgctccg
     4501 ctaggccggc acctaaaacc agcgccagga tcgctccaag tggagcaaaa ccgctaaagt
     4561 ttttaatcac attgggtaaa aaccagtgta atccttccac actgagcagg tttttcacca
     4621 ccaccggcgt accatcggtc gggtttttcg cactgacgcc aaaggctgac aaaattgccg
     4681 tcgtcaccat gagtacgatt atcaaataga taaagagcaa aaaaggatgg ggaaccttgt
     4741 taccaattct ttcgacccag ccatagagct tcccggattg ggaggacgac ggtatggatg
     4801 acatactcat gggcattcct cggttgttgt gtttgcgttg ttgttgttat tttaaaggtg
     4861 acggtgtcac gtttttcggg atagggcagt gatacggttg cgtgtccgta acttgctgat
     4921 gctcttgttg gcattcttgc aatagccctg aatcaatgaa gagattgact gtggttgctg
     4981 ccatagtttt cgccgccagc agcattcctt tatgagcaat agatgttcgc ccctggctaa
     5041 ccagttgcca cgtatgtagc ggtgtaccga cggcaaaaca ggggctgaaa cactgggcaa
     5101 caggcagttt ccaactgacg tcgccgacat cagtcgatgc cgcaagcacg ttatcggtgg
     5161 cggcatatgg agcgacctca ttcgccagta ccgtttcacg atgacgtagt gcaaaagcct
     5221 tgccgttttc gccaccggtt gcggcgatat tattcagact gttttgccga tcgttggggg
     5281 tgagcgtagc ctgaatttgt ttcgcaaaag ccagttcttc ggagttccat tccggggtac
     5341 caaaatggga tagggcatgg tacatggcat tttctaaggt gcgattcggg agataactgg
     5401 aacaggcttt gtcgaagcgg cattcaaccg tggtttcggt catcaatgcc gcaccttcgg
     5461 cgattttggc gacccgatca taaatatgct gcacgtcggt catttcgggg gcgcggataa
     5521 gataaagcac ttctgcctgc gcctggacca cgttgggcga gatcccgccg ctatttgtga
     5581 tggcatagtg tacgcgcgct ttttcaataa tatgttcgtt gaggaagttg gtgccagtgg
     5641 tcatcaacgt tacggcatca agggcgctgc gtcccaaatg aggggaattc gcggcatgtg
     5701 ctgcgatccc tttaaagcac catgatgcct gaatgtttgc cagcgtgcgg gtattgaaca
     5761 tgccggcaaa ggcttccggg tgccaggtga gtgccgcatc cacatcatca aataccccct
     5821 cgcggaccat gaacgtttta cccgagccgc cttcttcgcc aggacaacca taaaagcgca
     5881 ctgtgccgcc ttgcccatat tgttccagcc atttcttgac ggctattgca gcggcaaagg
     5941 cggcggttcc cagcaaattg tgtccgcaac cgtgaccatt ttcaccgggc gtcgcggatg
     6001 taggttgcgc gcaaccagct tgctgactta aacctgccag ggcgtcatat tctcccagca
     6061 gggcgataac cggtttgcct tgaccaaacg aagcaataaa tgcatttggg atattgccta
     6121 cgttgcgggt aacggtgaag cttgcagatt ccagcgccga agccagatgc tccgctgacc
     6181 agaactcttc aaaacgtgtt tctggatgat cccagatttg atcggcaata tcggtataac
     6241 gttggcgatc ggcttcaatc gcatcgtcga taaaacgata gatttcctgc atcagatacc
     6301 tcgcgtccag ggaaaattga gcgcggtgcg cgccagcgtt tcgacggcaa tagcgagaac
     6361 ctgctcgtca aaatcgaatt tttcgttgtg atgacctgcc gccagctgtg tgccaaacac
     6421 cacgtaggag gcttgccctt gatggtgctg cacgcgggcc atcattaatg tggcatcttc
     6481 ggaacccgca ggcgcttcaa cacgttcaat ggcctgattg acccccgcga cctgagccgc
     6541 ctgactttgc aaccatgcga cccattgcgg cgaaggagaa ctggcggtag ctgcacccat
     6601 caggcgagtt tcaacgccga caccatacat ggttgctgcg ccctgaatcg cttgttgtgc
     6661 acggtcaaaa acatattgat taatgacgtc gctggccccg cgtgtttcca ctttcagcaa
     6721 cgccgaggca ggaacaacgt tacggccgct tcctgcctgc ataacgccca cgtttactct
     6781 ggaagctcct tcgctgtgcg gggcgattgc atgcagtgca agagttgctt gtgctgccgc
     6841 caacaaggca ttgtgaccgt cttctggttt tgcgcctgcg tgagcggcgg tcccggtgaa
     6901 gtgcgcgtca aatttggtgg ttgccataaa attatcactg ccgcacacca cggtgcccgc
     6961 aggtacgcca gtgccaatgt gcacggcagt aaaataatca acatcatcta cgacacctgc
     7021 atcgaccatc gcccgcgcgc cacgcgtacc ttcctctgca ggctgaaaaa tcagtttgat
     7081 gacgccatgt agtccggact cgaattgttt aagggtatgc gccagcccaa gcccaatggc
     7141 ggtatgccca tcatgaccac aggcatgcat cattccggcg ttacatgacg caaaaccgtc
     7201 gcggtagggg cgatggctga catcctgctc ttcactgaga tccagcgcgt ccatatcgac
     7261 acggaaagcc ataaccggac cggggcgacc ggtatccagg gtggcgacga tacccgtgaa
     7321 accaccttca aaaaccgcaa tccattgtgc tagcgcaccc tgttgacgag cgcgctcgaa
     7381 ttcgcgttgt agagtgagtt catcaggtag tcccatccgg ctactttcat taactacttc
     7441 gcggcccagc gccagtgaat agccgagctg ctgcaattct tccgcaacaa gggtggcagt
     7501 gcggaattcc acccagccag actctgcata gtgatgaaaa tcacgtcgcc agtgcgataa
     7561 ttttggggca agcgaattaa caaattgatt caaagactcc ataacctttc ccgtcatcag
     7621 taaaaagtgt gacccggttc acgtagcgat agtttttact tatcactaac tgatttttca
     7681 cagttttaac cgttcataaa ttaccctgac acaatcatct gcattaaagt agatgccagt
     7741 ttctttggtc tgataaataa cggttatcgg tggcgtcatg gcttttcagg taaaaattca
     7801 tcaaattcgg gcttttgttg aagtggctcg tcagggcagc attcgcggag cgagccgaat
     7861 gttgaatatg tcgcaaccgg cactgagtaa atctattcag gagctggaag aaggattagc
     7921 ggcgcaactc ttttttcgcc gtagtaaagg cgtgacgtta actgatgccg gtgaaagttt
     7981 ttatcagcac gccagtctta ttcttgaaga gctgcgcgca gcgcaagagg aaattcgcca
     8041 acgacaaggg caactggcag ggcagattaa tatcggcatg ggggccagta tttcccgcag
     8101 tctgatgcca gctgtcatat ctcgttttca tcagcagcat ccgcaggtaa aagtacgcat
     8161 tatggaaggg caactggtgt cgatgattaa tgaattgcgt cagggagaat tggatttcac
     8221 catcaatacc tattatcagg gaccgtacga ccacgaattt acttttgaga aattactgga
     8281 aaagcaattc gcgatctttt gccgcccggg acaccccgcc attggtgccc gttcgatcaa
     8341 acagttactg gattacagct ggacaatgcc gacgccacac ggcagttact acaaacagtt
     8401 gagtgaattg cttgacgatc aggcgcaaac gccacaggtc ggtgtagtct gcgagacgtt
     8461 ctcagcctgt atcagtctgg tggcaaaaag cgattttctc agcatactgc ctgaagaaat
     8521 gggctgcgat cccttgcacg gacaggggct ggtgatgttg ccggttagcg aaattttacc
     8581 gaaagcggcc tattatttga ttcagcggcg tgatagtcgc cagacaccac tgaccgcgtc
     8641 attaatcacg caattccggc gagaatgcgg ctatctgcaa agttaaaact gcataaaaaa
     8701 atagagtctg tcgacatccg ccagactcta cagtacacac agcagtgcat ccgcgtctta
     8761 agtccggcat tgtctcctct gcgccggtga ctgtgttata acggttttaa cctttagttg
     8821 ccaattttct tcagtga
//
LOCUS       DAZFWL020000169         8794 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFWL020000169 DAZFWL020000000
VERSION     DAZFWL020000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.169"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(370..633)
                     /gene="rpsT"
                     /locus_tag="ACHKNP_RS21835"
                     /old_locus_tag="pgaptmp_004367"
     CDS             complement(370..633)
                     /gene="rpsT"
                     /locus_tag="ACHKNP_RS21835"
                     /old_locus_tag="pgaptmp_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGU4706417.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            736..954
                     /locus_tag="ACHKNP_RS21840"
                     /old_locus_tag="pgaptmp_004368"
     CDS             736..954
                     /locus_tag="ACHKNP_RS21840"
                     /old_locus_tag="pgaptmp_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HGU4706418.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            962..1903
                     /gene="ribF"
                     /locus_tag="ACHKNP_RS21845"
                     /old_locus_tag="pgaptmp_004369"
     CDS             962..1903
                     /gene="ribF"
                     /locus_tag="ACHKNP_RS21845"
                     /old_locus_tag="pgaptmp_004369"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGU4706419.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLGVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1946..4762
                     /gene="ileS"
                     /locus_tag="ACHKNP_RS21850"
                     /old_locus_tag="pgaptmp_004370"
     CDS             1946..4762
                     /gene="ileS"
                     /locus_tag="ACHKNP_RS21850"
                     /old_locus_tag="pgaptmp_004370"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGU4706420.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4762..5256
                     /gene="lspA"
                     /locus_tag="ACHKNP_RS21855"
                     /old_locus_tag="pgaptmp_004371"
     CDS             4762..5256
                     /gene="lspA"
                     /locus_tag="ACHKNP_RS21855"
                     /old_locus_tag="pgaptmp_004371"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGU4706421.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5344..5793
                     /gene="fkpB"
                     /locus_tag="ACHKNP_RS21860"
                     /old_locus_tag="pgaptmp_004372"
     CDS             5344..5793
                     /gene="fkpB"
                     /locus_tag="ACHKNP_RS21860"
                     /old_locus_tag="pgaptmp_004372"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4706422.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5795..6745
                     /gene="ispH"
                     /locus_tag="ACHKNP_RS21865"
                     /old_locus_tag="pgaptmp_004373"
                     /gene_synonym="lytB"
     CDS             5795..6745
                     /gene="ispH"
                     /locus_tag="ACHKNP_RS21865"
                     /old_locus_tag="pgaptmp_004373"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HGU4706423.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6811..7725
                     /gene="rihC"
                     /locus_tag="ACHKNP_RS21870"
                     /old_locus_tag="pgaptmp_004374"
     CDS             6811..7725
                     /gene="rihC"
                     /locus_tag="ACHKNP_RS21870"
                     /old_locus_tag="pgaptmp_004374"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HGU4706424.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7892..8713
                     /gene="dapB"
                     /locus_tag="ACHKNP_RS21875"
                     /old_locus_tag="pgaptmp_004375"
     CDS             7892..8713
                     /gene="dapB"
                     /locus_tag="ACHKNP_RS21875"
                     /old_locus_tag="pgaptmp_004375"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGU4706425.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         2010 a         2165 c         2493 g         2126 t
ORIGIN      
        1 gtgttgattg ttgttaatta tttacaagat ctaataagga gatatttatg aatggttaaa
       61 gtgtatagga atttaattca aaaatagagt ggtcttaagt ttaatacctc aatgtgtatc
      121 acaatatcca cattctttgt aggggagtct ggagattgag tagatattct tgttcagaat
      181 gtatcagccg atggttctac gattcttaag ccacgaagag ttcagaaagt gtaacggcat
      241 gtctgcaaaa gccagcatct tttgactatc tggcaaccgg tagtgtgttc tctcacgcat
      301 cataaaagca gcaggcataa aaaaacccgc ttgcgcgggc tttttcacaa agcttcagca
      361 aattggcgat taagccagtt tgttgatctg tgcagtcagg ttagccttat gacgtgcagc
      421 tttgtttttg tggatcagac ctttagcagc ctgacggtcc acgatcggtt gcatttcgtt
      481 aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata gctgcgtata ctttcttgat
      541 gaaagtacgc atcatagagc gacggcttgc gttgtgctta cgagcctttt cagactgaat
      601 ggcgcgcttc ttagctgatt tgatattagc caaggtccaa ctcccaaatg tgttctatat
      661 ggacaattca aaggccgagg aatatgccct tttagccttc ttttgtcaat ggatttgtgc
      721 aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg atggcgcagg attctaccag
      781 cttgcagggt gtgaatacag cttttccgcg ataaaaattg cagcaggcgg tcactttctt
      841 cccgtgattt gcgccatggc aatgaaaagc cacttctttc tgatttcggt actcaatcgc
      901 cggttaacct tgaccgctgt acaaggtata ctcggacgat tttcactgtt ttgagccaga
      961 catgaagctg atacgcggca tacataatct cagccaggcc ccgcaagaag ggtgtgtgct
     1021 gactattggt aatttcgacg gcgtgcatcg cggtcatcgc gcgctgttac agggcttgca
     1081 ggaagaaggg cgcaagcgca acttaccggt gatggtgatg ctttttgaac ctcaaccact
     1141 ggaactgttt gctaccgata aagccccggc acggctgacc cggctgcggg aaaaactgcg
     1201 ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg cgtttcgaca ggcgtttcgc
     1261 ggcgttaacc gcgcaaaatt tcatcagcga tcttctggtg aagcatttgc gcgtaaaatt
     1321 tcttgccgta ggtgatgatt tccgctttgg cgctggtcgt gaaggcgatt tcttgttatt
     1381 acagaaagct ggcatggaat acggcttcga tatcaccagt acgcaaactt tttgcgaagg
     1441 tggcgtgcgt atcagcagca ccgctgtgcg tcaggcgctg gcggatgaca atctggctct
     1501 ggcagaaagt ttactggggc acccgtttgc catctccggg cgtgtagtcc acggtgatga
     1561 attagggcgc actataggtt tcccgacggc gaatgtaccg ctacgccgtc aggtttcccc
     1621 ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt gaaaagccgt tacccggcgt
     1681 ggcaaacatt ggaacacgcc caacggttgc cggtattcgc cagcagctgg aagtgcattt
     1741 gttaggtgtt gcaatggacc tttacggtcg ccatatacaa gtagtgctgc gtaaaaaaat
     1801 acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa gcgcagattg cgcgtgatga
     1861 attaaccgcc cgcgaatttt ttgggctaac aaaaccggct taagcctgtt atgtaatcaa
     1921 accgaaacac ggaaccgaga atctgatgag tgactataaa tcaaccctga atttgccgga
     1981 aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa ccgggaatgc tggcgcgttg
     2041 gactgatgat gatctgtacg gcatcatccg tgcggctaaa aaaggcaaaa aaaccttcat
     2101 tctgcatgat ggccctcctt atgcgaatgg cagcattcat attggtcact cggttaacaa
     2161 gattctgaaa gacattatcg tgaagtccaa agggctttcc ggttatgact cgccgtatgt
     2221 gcctggctgg gactgccacg gtctgccgat cgagctgaaa gtagagcaag aatacggtaa
     2281 gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag tgccgcgaat acgcggcgac
     2341 ccaggttgac ggtcaacgca aagactttat ccgtctgggc gtgctgggcg actggtcgca
     2401 cccgtacctg accatggact tcaaaactga agccaacatc atccgcgcgc tgggcaaaat
     2461 catcggcaac ggtcacctgc acaaaggcgc gaagccagtt cactggtgcg ttgactgccg
     2521 ttctgcgctg gcggaagcgg aagttgagta ttacgacaaa acttctccgt ccatcgacgt
     2581 tgcttttcag gcggtcgatc aggatgcact gaaagcaaaa tttgccgtaa gcaacgttaa
     2641 cggcccaatc tcgctggtga tctggaccac tacgccgtgg actctgcctg cgaaccgcgc
     2701 aatctctatt gcacctgatt tcgactatgc gctggtgcag atcgacggtc aggccgtgat
     2761 tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc ggcgtgaccg attacaccat
     2821 tctcggcacg gtaaaaggtg cggagcttga gttgctgcgc tttacccatc cgtttatggg
     2881 cttcgacgtt ccggcaatcc tcggcgatca cgttaccctg gatgccggta ccggtgccgt
     2941 tcacaccgcg cctggccacg gcccggacga ctatgtgatc ggtcagaaat acggcctgga
     3001 aaccgctaac ccggttggcc cggacggcac ttatctgccg ggcacttatc cgacgctgga
     3061 tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg ctgctgcagg aaaaaggcgc
     3121 gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc tgctggcgtc acaaaacgcc
     3181 gatcatcttc cgcgcgacgc cgcagtggtt cgtcagcatg gatcagaaag gtctgcgtgc
     3241 gcagtcactg aaagagatca aaggcgtgca gtggatcccg gactggggcc aggcgcgtat
     3301 cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc cgtcagcgca cctggggcgt
     3361 accgatgtca ctgttcgtgc acaaagacac ggaagagctg catccgcgta ctctcgaact
     3421 aatggaagaa gtggcaaaac gcgttgaagt tgacggcatc caggcgtggt gggatcttga
     3481 tgcgaaagag atcctcggcg atgaagctga tcagtacgtg aaagtgccgg acacattgga
     3541 tgtatggttt gactccggat ctacccactc ttctgttgtt gacgtgcgtc cggaatttgc
     3601 cggtcacgca gcggacatgt atctggaagg ttctgaccag caccgtggtt ggttcatgtc
     3661 ttccctaatg atctccaccg cgatgaaagg caaagcgccg tatcgtcagg tactgaccca
     3721 cggctttacc gtggatggtc agggtcgcaa gatgtctaaa tccatcggca ataccgtttc
     3781 gccgcaggat gtgatgaaca aactgggcgc ggatattctg cgtctgtggg tggcatcaac
     3841 cgactacacc ggtgaaatgg ccgtttctga cgagatcctg aaacgtgctg ccgatagcta
     3901 tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac ctgaacggtt ttgatccagc
     3961 aaaagatatg gtgaaaccgg aagagatggt ggtactggat cgctgggccg taggttgtgc
     4021 gaaagcggca caggaagaca tcctcaaggc gtacgaagca tacgatttcc acgaagtggt
     4081 acagcgtctg atgcgcttct gctccgttga gatgggttcc ttctacctcg acatcatcaa
     4141 agaccgtcag tacaccgcca aagcggacag cgtggcgcgt cgtagctgcc agactgcgct
     4201 gtatcacatc gctgaagcgc tggtgcgctg gatggcacca atcctctcct tcaccgctga
     4261 tgaagtgtgg ggctatctgc cgggcgaacg tgaaaaatac gtctttaccg gtgagtggta
     4321 cgaaggcctg tttggtctgg cagacagtga agcgatgaac gatgcgttct gggacgagct
     4381 gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa gcgcgtgccg ataagaaagt
     4441 gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa ccggaactgg cggcgaaact
     4501 gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc tccggcgcta ccgttgcaga
     4561 ctataatgac gcacctgctg atgctcagca gagcgaagta ctcaaagggc tgaaagtcgc
     4621 gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg cactacaccc aggatgtcgg
     4681 caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc agcaacgtcg ccggtgacgg
     4741 tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt caacagggct acgctggctg
     4801 tggctggtgg tagtcgtgct gattatcgat ctgggcagca aatacctgat cctccagaac
     4861 tttgctctgg gggatacggt cccgctgttc ccgtcgctta atctgcatta tgcgcgtaac
     4921 tatggcgcgg cgtttagttt ccttgccgat agcggcggct ggcagcgttg gttctttgcc
     4981 ggtattgcga ttggtattag cgtgatcctg gcagtgatga tgtatcgctc gaaggccacg
     5041 cagaagctaa acaatatcgc ttatgcgctg attattggcg gcgcgctggg caacctgttc
     5101 gaccgcctgt ggcacggctt cgttgtcgat atgatcgact tctacgtcgg cgactggcac
     5161 ttcgccacct tcaaccttgc cgatactgcc atttgtgtcg gtgcggcact gattgtgctg
     5221 gaaggttttt tgccttctaa agcgaaaaaa caataataaa ccctgccgga taaggcctac
     5281 agattgctgc gaaatcgtag gccgcatccg gcaaaaatcc tcaaaatata agagcaaacc
     5341 tgcatgtctg aatctgtaca gagcaatagc gccgtcctgg tgcacttcac gctgaaactc
     5401 gatgatggca ccaccgctga gtctacccgc aacaacggta aaccggcgct gttccgcctg
     5461 ggcgatgctt ctctttctga agggctggag caacacctgt tggggctgaa agtgggcgat
     5521 aaaaccacct tctcgctgga gcccgatgcg gcgtttggcg tgccgtcacc ggacctgatt
     5581 cagtacttct cccgccgtga atttatggat gcaggcgagc cagaaattgg cgcaattatg
     5641 ctttttaccg caatggatgg cagtgagatg cctggcgtga tccgcgaaat taacggcgac
     5701 tccattaccg ttgatttcaa ccatccgctg gccgggcaga ccgttcattt tgatattgaa
     5761 gtgctggaaa tcgatccggc actggaggcg taacatgcag atcctgttgg ccaacccgcg
     5821 tggtttttgt gccggggtag accgcgctat cagcattgtt gaaaacgcgc tggccattta
     5881 cggcgcaccg atatatgtcc gtcacgaagt ggtacataac cgctatgtgg tcgatagctt
     5941 gcgtgagcgt ggggctatct ttattgagca gattagcgaa gtaccggacg gcgcgatcct
     6001 gattttctcc gcacacggtg tttctcaggc ggtacgtaac gaagcaaaaa gtcgcgattt
     6061 gacggtattt gacgcgacct gtccgctggt gaccaaagtg catatggaag tcgcccgcgc
     6121 cagtcgccgt ggcgaagaat ctattctcat cggtcacgcc gggcacccgg aagtggaagg
     6181 gacaatgggc cagtacagta acccggaagg gggaatgtat ctggtcgaat cgccggacga
     6241 tgtgtggaaa ctgacggtca aaaacgaaga gaagctctcc tttatgaccc agaccacgct
     6301 gtcggtggat gacacgtctg atgtgatcga cgcgctgcgt aaacgcttcc cgaaaattgt
     6361 cggtccgcgc aaagatgaca tctgctacgc cacgactaac cgtcaggaag cggtacgcgc
     6421 cctggcagaa caggcggaag ttgtgttggt ggtcggttcg aaaaactcct ccaactccaa
     6481 ccgtctggcg gagctggccc agcgtatggg caaacgcgcg tttttgattg acgatgcgaa
     6541 agatatccag gaagagtggg tgaaagaggt taaatgcgtc ggcgtgactg cgggcgcatc
     6601 ggctccggat attctggtgc agaatgtggt ggcacgtttg cagcagctgg gcggtggtga
     6661 agccattccg ctggaaggcc gtgaagaaaa cattgttttc gaagtgccga aagagctgcg
     6721 tgtcgatatt cgtgaagtcg attaagtcat tagcagccta agttatgcga aaatgccggt
     6781 cttgttaccg gcatttttta tggagaaaac atgcgtttac ctatcttcct cgatactgac
     6841 cccggcattg acgatgccgt cgccattgcc gccgcgattt ttgcacccga actcgacctg
     6901 caactgatga ccaccgtcgc gggtaatgtc tcggttgaga aaactacccg caatgccctg
     6961 caactgctgc atttctggaa tgcggagatt ccgctcgccc aaggggccgc tgtgccgctg
     7021 gtacgcgcac cgcgtgatgc ggcatctgtg cacggcgaat cgggaatggc tggctacgac
     7081 tttgttgagc acaaccgaaa gccgctcggg ataccggcgt ttctggcgat tcgggatgcc
     7141 ctgatgcgtg caccagagcc tgttaccctg gtggccatcg gcccgttaac caatattgcg
     7201 ctgttacttt cacaatgccc ggaatgcaag ccgtatattc gccgtctggt gatcatgggt
     7261 ggttctgccg gacgcggcaa ctgtacgcca aacgccgagt ttaatattgc tgccgatcca
     7321 gaagctgctg cctgtgtctt ccgcagtggt attgaaatcg tcatgtgcgg tttggatgtc
     7381 accaatcagg caatattaac tcctgactat ctctctacac tgccgcagtt aaaccgtacc
     7441 gggaaaatgc ttcacgccct gtttagccac taccgtagcg gcagtatgca aagcggcttg
     7501 cgaatgcacg atctctgcgc catcgcctgg ctggtgcgcc cggacctgtt cactctcaaa
     7561 ccctgttttg tggcagtgga aactcagggc gaatttacct caggcacgac ggtggttgat
     7621 atcgacggtt gcctgggcaa gccagccaat gtacaggtgg cattggatct ggatgtgaaa
     7681 ggcttccagc agtgggtggc tgaggtgctg gctctggcgt cgtaacctgt cacatgttat
     7741 tggcatgcag tcattcatcg actcatgcct ttcactgata tccctccctg tttatcatta
     7801 atttctaatt atcagcgttt ttggctggcg gcgtagcgat gcgctggtta ctctgaaaac
     7861 ggtctatgca aattaacaaa agagaatagc tatgcatgat gcaaacatcc gcgttgccat
     7921 cgcgggagcc ggggggcgta tgggccgcca gttgattcag gcggcgctgg cattagaagg
     7981 cgtgcagctg ggcgcggcgc tggagcgcga aggatcttct ttactgggca gcgacgccgg
     8041 tgagctggct ggtgcaggga aaacaggcgt taccgtgcaa agcagcctcg atgcggtaaa
     8101 agatgatttt gatgtgttta tcgattttac ccgtccggaa ggtacgctga accatctcgc
     8161 tttttgtcgc cagcatggca aagggatggt gatcggcact acggggtttg acgaagccgg
     8221 taaacaggcg attcgtgacg ccgccgccga tattgcgatt gtctttgccg ccaattttag
     8281 cgttggcgtt aacgtcatgc ttaagctgct ggagaaagca gccaaagtga tgggtgacta
     8341 caccgatatc gaaattattg aagcacatca tagacataaa gttgatgcgc cgtcaggcac
     8401 cgcactggca atgggagagg cgatcgccca cgcccttgat aaagatctga aagattgcgc
     8461 ggtctacagt cgtgaaggcc acaccggtga acgtgtgcct ggcaccattg gttttgccac
     8521 cgtgcgtgca ggtgacatcg ttggcgaaca taccgcgatg tttgccgata ttggcgagcg
     8581 tctggagatc acccataagg cgtccagccg catgacattt gctaacggcg cggtaagatc
     8641 ggctttgtgg ttgagtggta aggaaagtgg tctttttgat atgcgagatg tgcttgatct
     8701 caatagtttg taaccacaaa atatttgtta tggtgcaaaa ataacacatt taatttattg
     8761 attgtaaagg gctttaattt ttggcccttt tatt
//
LOCUS       DAZFWL020000170         8790 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFWL020000170 DAZFWL020000000
VERSION     DAZFWL020000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.170"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..142)
                     /locus_tag="ACHKNP_RS21880"
                     /old_locus_tag="pgaptmp_004376"
     CDS             complement(<1..142)
                     /locus_tag="ACHKNP_RS21880"
                     /old_locus_tag="pgaptmp_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGU4706426.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTA"
     gene            complement(553..1533)
                     /locus_tag="ACHKNP_RS21885"
                     /old_locus_tag="pgaptmp_004377"
                     /pseudo
     CDS             complement(553..1533)
                     /locus_tag="ACHKNP_RS21885"
                     /old_locus_tag="pgaptmp_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1830..2432
                     /locus_tag="ACHKNP_RS21890"
                     /old_locus_tag="pgaptmp_004378"
     CDS             1830..2432
                     /locus_tag="ACHKNP_RS21890"
                     /old_locus_tag="pgaptmp_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HGU4706427.1"
                     /translation="MKNDKAWIGDLLGGPLMSRESRVIAELLLTDPDEQTWQEQIVGH
                     NILQASSPNTAKRYAATIRLRLNTLDKSAWTLIAEGSERERQQLLFVALMLHSPVVKD
                     FLAEVVNDLRRQFKEKLPGNSWNEFVNSQVRLHPVLASYSDSSIAKMGNNLVKALAEA
                     GYVDTPRRRNLQAVYLLPETQAVLQRLGQQDLISILEGKR"
     gene            2429..3031
                     /locus_tag="ACHKNP_RS21895"
                     /old_locus_tag="pgaptmp_004379"
     CDS             2429..3031
                     /locus_tag="ACHKNP_RS21895"
                     /old_locus_tag="pgaptmp_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HGU4706428.1"
                     /translation="MIDPVLEYRLSQIQSRINEDRFLKNNGSGNEIGFWIFDYPAQCE
                     LQVREHLKYLLRHLEKDHKFACLNVFQIIIDMLNERGLFERVCQQEVKVGTETLKKQL
                     AGPLNQKKIADFIAKKVDLAAQDFVILTGMGNAWPLVRGHELMSALQDVMGFTPLLMF
                     YPGTYSGYNLSPLTDTGSQNYYRAFRLVPDTGPAATLNPQ"
     gene            3043..6684
                     /gene="brxC"
                     /locus_tag="ACHKNP_RS21900"
                     /old_locus_tag="pgaptmp_004380"
     CDS             3043..6684
                     /gene="brxC"
                     /locus_tag="ACHKNP_RS21900"
                     /old_locus_tag="pgaptmp_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HGU4706429.1"
                     /translation="MNIEQIFEKPLKRNINGVVKAEQTDDASAYIELDEYVITRELEN
                     HLRHFFESYVPATGPERIRMENKIGVWVSGFFGSGKSHFIKILSYLLSNRKVTHNGTE
                     RNAYSFFEDKIKDALFLADINKAVHYPTEVILFNIDSRANVDDKEDAILKVFLKVFNE
                     RIGYCADFPHIAHLERELDKRGQYETFKAAFADINGSRWEDERDAYYFISDDMAQALS
                     QATQQSLESSRQWVEQLDKNFPLDINNFCQWVKEWLDDNGKNILFMVDEVGQFIGKNT
                     QMMLKLQTITENLGVICGGRAWVIVTSQADINAAIGGMSSRDGQDFSKIQGRFSTRLQ
                     LSSSNTSEVIQKRLLVKTDEAKAALAKVWQEKADILRNQLAFDPTTTTALRPFTSEEE
                     FVDNYPFVPWHYQILQKVFESIRTKGAAGKQLAMGERSQLEAFQTAAQQISAQGLDSL
                     VPFWRFYAAIESFLEPAVSRTITQACQNGILDEFDGNLLKTLFLIRYVETLKSTLDNL
                     VTLSIDRIDADKVELRRRVEKSLNTLERLMLIARVEDKYVFLTNEEKEIENEIRNVDV
                     DFSAINKKLASIIFDDILKSRKYRYPANKQDFDISRFLNGHPLDGAVLNDLVVKILTP
                     KDPTYSFYNSDATCRPYTSEGDGCILIRLPEEGRTWSDIDLVVQTEKFLKDNAGQRPE
                     QATLLSEKARENSNREKLLRVQLESLLAEADVWAIGERLPKKSSTPSNIVDEACRYVI
                     ENTFGKLKMLRPFNGDISREIHALLTVENDTELDLGNLEESNPDAMREVETWINMNIE
                     YNKPVYLRDILNHFARRPYGWPEDEVKLLVARLACKGKFSFSQQNNNVERKQAWELFN
                     NSRRHSELRLHKVRRHDEAQVRKAAQTMADIAQQPFNEREEPALVEHIRQVFEEWKQE
                     LNVFRAKAEGGNNPGKNEIESGLRLLNAILNEKEDFALIEKVSSLKDELLDFSEDRED
                     LVDFYRKQFATWQKLGAALNGSFKSNRSALEKDAAAVKALGELESIWQMPEPYKHLNR
                     ITPLIEQVQNVNHQLVEQHRQHALERIDARIEESRQRLLEAHATSELQNSVLLPMQKA
                     RKRAEVSQSIPEILAEQQETKALQMDADKKINLWIDELCKKQEAQLRAANEAKRAADS
                     EQTYVVVEKTVIQPVPKKTHLVNVASEMRNAAGGEVLETTEQVEKALDTLRTTLLAAI
                     KAGDRIRLQ"
     gene            6730..>8790
                     /gene="pglX"
                     /locus_tag="ACHKNP_RS21905"
                     /old_locus_tag="pgaptmp_004381"
     CDS             6730..>8790
                     /gene="pglX"
                     /locus_tag="ACHKNP_RS21905"
                     /old_locus_tag="pgaptmp_004381"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HGU4706430.1"
                     /translation="MNTNNIKKYAPQARNDFRDAVIQKLTTLGIAADKKGNLQIAEAE
                     TIGETVRYGQFDYPLSTLPRRERLVKRAREQGFEVLVEHCAYTWFNRLCAIRYMELHG
                     YLEHGFRMLSHPETPTAFEVLDHVPEVAEALLPENKAQLVEMKLSGNQDEALYRELLL
                     GQCHALHHVMPFLFEAVDDEAELLLPDNLTRTDSILRGLVDNIPEEDWEQVEVIGWLY
                     QFYISEKKDAVIGKVVKSEDIPAATQLFTPNWIVQYLVQNSVGRQWLQTYPDSPLKDK
                     MEYYIEPAEQTPEVQAQLAAITPASIEPESIKVLDPACGSGHILIEAYNVLKNIYEER
                     GYRGRDIPQLILENNIFGLDIDDRAAQLSGFALLMMARQDDRRIFTRDVRLNIVSLQE
                     SLHLDIAKLWQQLNFHQQVQTGSMGDMFAENTALTQTNSAEYQLLMRTLKRFVNAKTL
                     GSLIQVPQEEEAELKVFLDALYRLEQEGDFQQKSAAKAFIPFIQQAWILAQRYDAVVA
                     NPPYMGSKYQSLSVKKHLKDNFTGYDKDLFSAFIVRNLELSKQGAQLGFMTPFVWMFI
                     TTYEELRKKLIEQEIITSLVQLEYSGFDGATVPICTFTLQKGHINHFTGSYIRLSDFR
                     GASNQGPKTLEAIQHHNCGWFYEAQPDNFKKIPGTPIAYWLGQNINIFDNDKIANSWF
                     SGGRI"
BASE COUNT         2464 a         2035 c         2050 g         2241 t
ORIGIN      
        1 aggcggtgta agtgaactgt agttgccatg ttttacggca atgagagcag agatagcgct
       61 gatgtccggc agtgcttttg ccgttacgca ccacgccttc agtagcggag caggaaggac
      121 atctgatgga aatggaagcc acgcaagcac cttaaaatca ccatcataca ctaaatcagt
      181 aagttggcag cattaccagc agatgtaaag aataccgctt tgttaatctg acttgtagac
      241 ttctttggga ttgtatcact ttcactcata atcatttatt ccattaatta aattcgcact
      301 ggatagaagg tactacctct cacagagtag caaagttcaa ctggctatgc ggcgttaatc
      361 ggaatgcatg gtggaatgtg aatcgccacc ggttttttag ggggttgtag agtggcatcg
      421 gaaagaggtt tttagggggg gaggttgata ataccgcgcc cccataatct tgccatctct
      481 tacattgaga tgttaaaagc tacaaatgct ctccctattt ttcaatgtag acatttatag
      541 caatataaaa atttattgtt cagctttttc aacaacaaaa ccattttgcc aaacaaacac
      601 aatcaagagt tcaatattaa caatacccca ttgattagaa atggcgacaa tttcaccaga
      661 aggaagtgta atcaatgcat cttcgttgat atgatagcga ctcttttgct tctctggtat
      721 ctgatcgata agtataaccg tacgtttttt aaagtcttca cttaatccat ttattaaatc
      781 attataactt gcagggttaa attgttttat acaatcgcgt aataactcaa gcgctaactt
      841 acgtttatta agttttttat ctttgcatat ataccacgta tagcccttct cttttttctg
      901 actgctaatt agctgctcat cacgtttttc acgaaactta acttgatatt cttccaattc
      961 aggaaccggt atgatttgct ctgcattgat cagaacatca tcattaaatc tataaggcgt
     1021 cagacgaaca catcgaatgt ctaccccttt atctcttaac caaatcgcag tggtagtcag
     1081 ttccttgcta aaatccgatg aagccaaaac gattctgata tctttaccaa aatcaacaag
     1141 ctcagtttca tccagctcaa caaattcaag tattttttct ttcgcatttt ttctttcgca
     1201 tcggcatcgc ggttttgttt tttcaaatac gtctgaaaat actcacatgc tttcgcaaaa
     1261 ctcattgtgt aaatcattgc cgcatatcgt agtgcctgta actccatatg cgtccccgtt
     1321 tcatcacgtt ttaattcgat aaccactata tttgcctgtt tatcgattgc caataaatca
     1381 attcgccgat ggctatcttc ccagtcagaa atcaccagac aatcaggagc aataatatct
     1441 atattttttg attgcttcct gtaatccttc cagagtaaag gtcgtttttt ctaaagcatg
     1501 tggttcttta tttgcgatat tgtacagcac cacgttcatc ttcctttttt aactgatttt
     1561 acagagactt taatacagtt aaaattttat ttcctgagct gtaatcgatt aagttgatgc
     1621 atttaatggg aatgatatag ggtcatttcc agtctcactt atagaaatgg ctaaagcatg
     1681 actctcgcca aaaccgttta tgtgttgtac ataacgcgat catccctctc acaaattgcc
     1741 ttttctcatg gcatctcgcc cggtccccca ttacaatcac tttttgtttt ttgcgagctg
     1801 cattccagtc ttcagagggt ttttcgatga ttaaaaatga caaggcatgg ataggagact
     1861 tgctgggcgg accgctcatg agcagggaaa gccgcgtcat tgccgaactg ttgctaaccg
     1921 atcccgatga acagacatgg caagagcaaa ttgttggcca caacatttta caagcctctt
     1981 ctcctaacac cgcaaaacgt tacgcggcaa caatcaggct tcgcctgaac acgctggata
     2041 aaagcgcgtg gacattgatt gccgaaggta gtgaacggga acgccaacaa cttctgtttg
     2101 tggctctgat gctacattcg ccggtagtta aggattttct ggctgaagtg gtgaacgatc
     2161 tgcgcaggca gttcaaggaa aagttgcctg gcaatagctg gaacgaattt gtgaatagcc
     2221 aggttcgcct acatccggta ctcgccagct actcagattc atctattgca aaaatgggaa
     2281 acaatctggt gaaggcgctt gctgaagcgg gttatgtgga tacgccccgc agacgtaacc
     2341 tgcaggcagt ttacctttta ccggaaactc aggcagtgtt acagcgcctg ggacaacagg
     2401 acttgatatc tattctggag ggaaaacggt gatagatccc gttcttgaat atcgcctgtc
     2461 tcaaatccag agtcgcatta acgaagatcg cttcctcaaa aataacggct ccggaaatga
     2521 aattggtttt tggatctttg attatcccgc gcagtgcgaa ctgcaggtac gggagcattt
     2581 gaaatatctg ctccggcatc tggaaaagga ccataaattt gcctgtctga atgtcttcca
     2641 aatcatcatc gatatgctca atgaacgcgg ccttttcgag cgcgtctgcc agcaggaagt
     2701 caaagtgggt actgagacgc tgaaaaagca gcttgctggt ccgttaaatc agaaaaagat
     2761 cgctgatttt atagcgaaaa aagtcgatct ggctgcccag gattttgtca ttcttaccgg
     2821 catgggcaac gcctggccat tagtacgcgg tcatgaactg atgagtgcct tgcaggatgt
     2881 catggggttc accccactgc tgatgtttta tcctggcacc tacagcgggt acaacctttc
     2941 cccgctcaca gacaccggtt cacaaaatta ttatcgcgct ttcagactgg taccagatac
     3001 gggacccgca gcaacattga atcctcaatg aagagcataa caatgaatat tgaacagatt
     3061 tttgaaaaac ctctaaaacg aaatataaac ggggtagtca aagcagagca aaccgatgat
     3121 gccagcgcgt acatcgagtt agatgaatat gtcatcaccc gcgaactgga aaaccatctt
     3181 cgccatttct tcgaatccta tgttcctgcc actggcccgg aacggatccg tatggaaaac
     3241 aagatcggcg tatgggtttc aggcttcttc ggttcaggta aatcgcactt tattaagatt
     3301 ctttcttatc ttttatctaa ccgcaaagtt acacataacg gtacggaacg taatgcttac
     3361 tccttctttg aagataaaat caaagatgca ttattccttg ccgatattaa caaagcggtg
     3421 cattacccga ctgaagtcat tctgttcaat attgattcgc gtgccaacgt agatgacaaa
     3481 gaagatgcca ttcttaaagt cttcctgaaa gttttcaacg aacgcattgg atactgcgct
     3541 gattttccgc atattgccca tcttgagcgc gagctggata aacgcggtca gtatgaaacc
     3601 tttaaagccg cgtttgccga tatcaatggc tcgcgctggg aagacgagcg cgacgcttac
     3661 tacttcatca gcgatgacat ggcacaagca ttaagccagg ccacgcagca gagtcttgaa
     3721 tcctcccgcc aatgggtgga acaactcgac aaaaacttcc cgctggatat caataatttt
     3781 tgccagtggg taaaagagtg gctggatgac aatggtaaga acatcctctt tatggtggat
     3841 gaagtcggtc agttcattgg caaaaatacg caaatgatgc tgaagctgca gactattact
     3901 gaaaaccttg gggtaatttg cggtggccgc gcatgggtta tcgtgacttc gcaggccgat
     3961 atcaacgcgg caatcggtgg tatgagcagt cgcgacggac aggacttctc caagatccag
     4021 gggcgcttct ctacacgcct gcaactttcc agctctaaca catcagaagt tatccagaaa
     4081 cgtttgttgg taaagactga cgaagcaaaa gcggcactgg caaaagtgtg gcaagagaaa
     4141 gccgatatcc tgcgtaacca gctggctttt gaccctacaa caactactgc actacgtcct
     4201 tttaccagcg aagaagagtt cgttgacaac tacccgtttg tcccgtggca ctatcagatt
     4261 ctgcaaaaag tgtttgaatc tattcgaacg aaaggtgcag cgggtaaaca attggccatg
     4321 ggtgagcgtt ctcagctgga ggcattccag acggcggcgc agcaaatctc agcgcaaggg
     4381 ctggattctc tggtgccttt ctggcgcttc tatgccgcca ttgagagctt cctggaacct
     4441 gccgttagcc gcaccatcac tcaggcttgc cagaatggca ttcttgatga attcgatggc
     4501 aacctgctta aaacgctgtt cctgatccgc tatgtggaaa cgctgaaaag caccctggat
     4561 aacctggtca cattgtctat cgataggatc gatgccgata aagttgagtt gcgccgccgg
     4621 gtcgaaaaaa gtctgaacac gcttgaacgc ctgatgctca ttgcgcgcgt tgaagataaa
     4681 tatgtgttcc tgaccaacga agaaaaagag atcgaaaacg agatccgtaa cgttgatgtc
     4741 gatttctctg cgatcaacaa aaaactggca tcgatcatct ttgatgacat tctgaaaagc
     4801 cgtaaatatc gttatccggc taacaagcaa gactttgata tcagtcgctt cctgaacgga
     4861 cacccattag acggcgcagt gcttaacgat ctggtagtga agatcctgac ccctaaagat
     4921 ccgacctatt cgttctataa cagcgatgcg acctgtcgcc cttatacgtc agaaggcgac
     4981 ggctgtattt tgattcgtct gcccgaagag ggccgtacct ggagcgatat tgatttagtc
     5041 gtccagactg aaaagttcct caaagataac gccgggcaac gtccggaaca ggcaaccctg
     5101 ctctcagaaa aagcgcgtga aaacagcaac cgggaaaaat tactccgtgt tcagttggaa
     5161 tcactacttg cagaagcaga tgtctgggcg attggcgagc gcttaccgaa aaaatcttcc
     5221 acaccatcga acattgtcga tgaagcctgc cgttacgtga ttgaaaacac cttcggcaag
     5281 ctgaagatgc tgcggccttt taacggcgac atctcccgtg aaattcatgc attactgacg
     5341 gttgagaacg acaccgaact ggatctcggt aacctcgaag agtccaaccc cgacgccatg
     5401 cgcgaggtag aaacctggat caacatgaat atcgaataca ataaacctgt gtatttacgc
     5461 gatattctga accatttcgc gcgtcgccct tatggctggc ccgaagacga agtgaaactg
     5521 ctggtagccc gtctggcctg caaaggtaag ttcagcttta gccagcaaaa caacaacgtc
     5581 gagcgaaaac aggcgtggga gttatttaat aacagccgcc gccatagcga attgcgtctg
     5641 cataaagttc gccgtcatga tgaagcgcag gtgcgtaaag ccgcgcaaac catggctgac
     5701 atcgctcagc agccgtttaa cgaacgggaa gagccggcgc tggttgaaca tattcgtcag
     5761 gtatttgaag agtggaagca agagctgaac gtattccgcg ccaaggcaga gggcggaaac
     5821 aatccgggga aaaacgagat tgaatccggt ctgcgcctgc ttaatgccat tcttaatgag
     5881 aaagaagatt ttgccctgat cgaaaaagtc tcatcgctga aagatgaact tctggatttc
     5941 agtgaagacc gtgaagattt ggtcgacttc taccgtaagc aattcgccac ctggcaaaaa
     6001 ctgggtgctg cgctgaatgg cagctttaaa tctaaccgca gcgcgctgga aaaagacgcc
     6061 gcagcggtta aagcgctggg tgagctggaa agcatctggc aaatgccgga accttataag
     6121 catctcaatc gcatcacgcc gttgattgaa caggtccaga acgtcaacca tcagttagtc
     6181 gaacagcacc gccagcacgc cctcgaacgc attgacgccc gcattgagga aagccgtcaa
     6241 cgcttgctgg aagcgcacgc cacgtcagaa ctgcaaaaca gcgttctgct gccgatgcaa
     6301 aaagccagaa aacgcgctga ggtcagccag tcgattccgg aaattttggc ggaacagcaa
     6361 gagacaaaag cgctgcaaat ggatgcagat aaaaagatta atctgtggat cgatgagctg
     6421 tgtaaaaagc aagaagcaca actccgggca gcaaatgaag ctaaacgcgc tgccgactca
     6481 gaacagactt atgttgtggt ggaaaaaacc gttatccaac cggtaccgaa aaaaacgcat
     6541 ctggtgaatg tcgccagtga gatgcgtaac gccgccggtg gtgaggttct ggaaaccacc
     6601 gaacaggtgg aaaaggcgct cgacacgtta cgcacaacgc tgctggccgc cattaaagca
     6661 ggcgatcgaa ttcgccttca gtaactccca tttcagggca gcactctgct gccctttgca
     6721 ggattttcta tgaatactaa taacattaaa aaatatgccc cacaggcccg taacgatttc
     6781 cgcgatgcgg tgattcagaa gctaacgacg cttgggatcg ctgcagataa aaaaggcaat
     6841 ttgcagattg ccgaggccga aaccattggc gagaccgtgc gttacggtca gtttgattac
     6901 ccgttatcga cccttccccg ccgcgaacgg ctggtaaaac gcgcccgtga gcagggtttt
     6961 gaggtgctgg ttgagcactg cgcctacacc tggtttaacc gcttatgtgc aattcgctat
     7021 atggagctac acggttatct tgagcacggc ttccgtatgt tgtcccaccc ggagacgccg
     7081 accgcgtttg aggtgctgga tcatgtgccg gaagtggcag aagccctgct gccggaaaat
     7141 aaggcgcagc tggttgaaat gaagctttcc ggtaatcagg acgaagccct gtaccgcgaa
     7201 ctgctgctgg ggcagtgcca cgccctgcac cacgtgatgc cgttcctgtt tgaagcggta
     7261 gatgacgaag cggaactgct gttgccggat aacctgaccc gtaccgactc tattctgcgt
     7321 gggctggttg ataatattcc tgaagaagac tgggagcagg tagaggttat cggctggctg
     7381 tatcagttct atatttcgga aaagaaagat gctgtgattg gcaaagtggt gaagagcgaa
     7441 gatattcctg ccgccaccca gctgtttacg ccaaactgga ttgtgcagta tctggtacag
     7501 aactccgttg gccgccagtg gttgcagacc tacccggact cgccgctgaa agacaaaatg
     7561 gagtactaca ttgagcctgc ggagcaaacg ccggaagtgc aggcacagct ggcggcgatt
     7621 accccagcca gcattgaacc cgaaagtatt aaagtgctcg acccggcctg cggttccggt
     7681 catattttga ttgaagccta taatgtgctg aaaaatatct acgaagagcg tggttatcgc
     7741 gggcgtgata ttccacaact gattctggaa aataatattt ttggtcttga tatcgacgac
     7801 cgcgcagccc aactttccgg ttttgcatta ttaatgatgg cgcgtcagga tgaccgcaga
     7861 atatttaccc gcgatgtacg tctgaatatt gtctctttgc aggaaagcct gcatctggat
     7921 atcgccaaac tctggcagca actgaatttc caccagcagg tacaaaccgg cagtatgggg
     7981 gatatgtttg ctgaaaatac cgcgttaacc caaactaaca gcgcagaata tcagctgctg
     8041 atgcgcacgt tgaaacgctt tgtgaatgca aaaacgctgg gatcgctgat tcaggtgccg
     8101 caggaagaag aagcggaact gaaagtattt ctggacgcgc tgtatcgcct ggaacaggaa
     8161 ggcgatttcc agcagaagtc ggcggcgaaa gcgtttattc cgtttattca gcaggcgtgg
     8221 attctggcac agcgatatga tgcggtggtg gcgaatccgc cgtatatggg aagcaaatac
     8281 caatcattaa gtgtaaaaaa acatcttaaa gataatttta ctggttatga taaggattta
     8341 ttttcagcct ttatagtcag aaacttagaa ctttcaaaac aaggggctca acttggattc
     8401 atgacgccat ttgtctggat gtttatcact acgtacgaag aattaagaaa aaaactgata
     8461 gagcaagaaa ttattacatc gcttgtgcag cttgaatatt caggctttga cggtgcaact
     8521 gtgccaattt gcacattcac attacagaaa ggtcatatta accattttac tggtagctat
     8581 atccgcctct ctgattttcg tggtgcatca aaccaaggtc ccaaaactct tgaagcgata
     8641 caacaccata attgtggatg gttttatgaa gcgcagccag acaattttaa aaaaatacca
     8701 ggaacaccaa tagcctattg gctaggccag aatataaata ttttcgataa tgacaaaata
     8761 gctaattcat ggttctctgg tggtagaatt
//
LOCUS       DAZFWL020000171         8687 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFWL020000171 DAZFWL020000000
VERSION     DAZFWL020000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.171"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(201..1325)
                     /gene="yhhJ"
                     /locus_tag="ACHKNP_RS21910"
                     /old_locus_tag="pgaptmp_004382"
     CDS             complement(201..1325)
                     /gene="yhhJ"
                     /locus_tag="ACHKNP_RS21910"
                     /old_locus_tag="pgaptmp_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4706431.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(1325..4060)
                     /gene="rbbA"
                     /locus_tag="ACHKNP_RS21915"
                     /old_locus_tag="pgaptmp_004383"
     CDS             complement(1325..4060)
                     /gene="rbbA"
                     /locus_tag="ACHKNP_RS21915"
                     /old_locus_tag="pgaptmp_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HGU4706432.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDSLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDITHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(4057..5124)
                     /gene="yhiI"
                     /locus_tag="ACHKNP_RS21920"
                     /old_locus_tag="pgaptmp_004384"
     CDS             complement(4057..5124)
                     /gene="yhiI"
                     /locus_tag="ACHKNP_RS21920"
                     /old_locus_tag="pgaptmp_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4706433.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSESRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARATLESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(5187..5300)
                     /gene="yhiY"
                     /locus_tag="ACHKNP_RS21925"
                     /old_locus_tag="pgaptmp_004385"
     CDS             complement(5187..5300)
                     /gene="yhiY"
                     /locus_tag="ACHKNP_RS21925"
                     /old_locus_tag="pgaptmp_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HGU4706434.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(5490..7112)
                     /gene="yhiJ"
                     /locus_tag="ACHKNP_RS21930"
                     /old_locus_tag="pgaptmp_004386"
     CDS             complement(5490..7112)
                     /gene="yhiJ"
                     /locus_tag="ACHKNP_RS21930"
                     /old_locus_tag="pgaptmp_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4706435.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSINYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(7374..>8687)
                     /locus_tag="ACHKNP_RS21935"
                     /old_locus_tag="pgaptmp_004387"
     CDS             complement(7374..>8687)
                     /locus_tag="ACHKNP_RS21935"
                     /old_locus_tag="pgaptmp_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4706436.1"
                     /translation="ADNTTDTFIFTIIPDNNHVLKLSSPITVTVECKGGYYFINSSGD
                     KSDITYKVNGLSIIPENFFTLLSGNFKADWRWDVSKETFPKDKFAGYVKSVFSKIDFY
                     KQCGVINPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLNHENMASS
                     AYKHKNNKVLYELLNTRDMILNELHKHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYI
                     LTLLNSMRNMESNKDSRINKNVVVLAGNHEINFNGNYTARLANHKLSAGDTYNLIKTL
                     DVCHYDSERKVLTSHHGIIRDEENACYCLGALQVPFKQMKNPTDPEELANIFNKKHKE
                     HMNDPLLHLIRSNIMKPTAVYADYINNTTDFRPKPERIFICGETLKCEDPSIYIRQKY
                     GHHGVGVDQNQQFDNGVMGLNSTKKVLDKNNKIIYSSGLSCFQSH"
BASE COUNT         2229 a         2232 c         1976 g         2250 t
ORIGIN      
        1 ctggcgtcag tccttgcggg gagcgggctt tcgtaatctt gccctgaata caggactgag
       61 tatcaaaaag ccggttaact gaaactgtcc aggttttggg ggtcagttca taaacgcctt
      121 atccggccta aaaacaacta aaattcaata aattgcaccg atgcgtagtc cctataagct
      181 tacgcatcgg gcaattgtat ttacgccatt gtcccaatcg tcttcctgaa tcgcagcagt
      241 gcaatggtga aaaacgcgcc gccaattgcc atcagcgtca ggaactgcgg ccagacgatt
      301 tcgaatccgg caccccggta gaggatggcc tgcgcgaggc taacaaagtg tgtcgtcggc
      361 atggtcagca taatgtcctg caccatctgc ggcatacttt cgcgcggcgt ggatccaccg
      421 gaaagcattt gcagcggcag cagcaccaga atcaccagca gccccagttg cggcattgaa
      481 cgcgctatcg tccccataaa aatgccgatt gacgtggtgg caaacagact gagcgccacg
      541 cccagcataa acagcgggat cgagccttca atcggtacac ccagcacgcc tttcaccatc
      601 agcaccagtg ataatcccga caccaccagc accaccagcc ccatcgacca gacctttgcc
      661 atcatgatct caaacggcgt tatcggcatc accaacaagt gttccaccgt gccgtgttca
      721 cgctcgcgga tcagcgccga tccggtcaat acaatcgcca gcatggtaat gttgttgatg
      781 attgccatca ccccgccaaa ccacgcggga tcaaggttcg ggttaaagcg catccgggtt
      841 tccagcgata ccaacggttc gctgttatca cggtagcgcg cgacaaagct gttcacttca
      901 ccgttgataa tattctggat atacccattg ccggtaaatg cctggctcat gcgcgtggca
      961 tcgacgttca cctgaatatc tggctggcgt ccggcgagga catcacgctg aaaattaggc
     1021 ggaatattta tcgcgaaggt atagcgtccg gcgtccagtc cggcatccat ctcatcggcg
     1081 gtgatcatct ccggtggcaa aaaccacgga cgatagaagc tgttaacgat ccggttcgat
     1141 aactgcgatt gatccatatc ggcaatggcg atcggcgcga ggttcaacga tcctggcgta
     1201 acggtcgctg acgaatacac cgacaccgta aacgagaaga caatcagcgt cagcatcgct
     1261 ttatcaccga gcagactgcg caactctttg atacccagat taaaaatatt gcgtaaatgg
     1321 cgcatcatcc ctcctgtttt ttcagcagca ggatacttaa gcccatcacc agcgggatgg
     1381 ctatcagcaa cgggataaaa agttgccaca aatcagtcag atccagcgct ttcgagaacg
     1441 tcccgcgggc gatagtcaga aaatgactgg tcgggtaaac ctcgccgatc caacgtccag
     1501 gcccttccag cgaagctacc ggatcgatca tcccggaaaa ctgtgtcgcc gggatcaacg
     1561 tgataatcgc cgttccgaaa atggcggcaa tctggctttt cataaaggtg gagatcagca
     1621 gccccattcc ggtggcaatg atgatataca gcagcgccgc cagggtgagc gtcaggaaac
     1681 tgcctttatg cggtacgcca aacacaaaca ccgacaggcc gcagagcagg aaaaagttca
     1741 gcatccccag cgcgatgtat ggcaactgtt taccaagcaa aaattcacta cgcgtggtgg
     1801 gggtcacgta aaggttgata atcgacccaa gctctttttc ccgcaccacg ctaagggcgc
     1861 ttagcattga cgggatcatc atcagcagaa gcgggatcac cgccggaaca atcgctggca
     1921 ggctttttac gtccgggtta tagcgataac gcgtctcaat gttcatcagc ccgctttggc
     1981 tggcgggtgt ggattgtcgg ctcgccacat cctgtaacca gctctggtgc attgcctgca
     2041 cgtaaccttt taccgtttcg gcacgactgg gcatcgctcc gtcaatccag acgccgagtt
     2101 ccacaggcgt accacgcgcg atatcgcgcc cgaaattggg agggatctca attgccaccg
     2161 tgatatcgcc cgcacgcatc cgacgatcaa gctcgtcata actggtgagc ggcggctgtt
     2221 cgataaagta acgggaaccg gagagattga gcgtccacgc ctggctactg acggtctggt
     2281 cgcggtcgag caccgcaaag cgcaggtttt ccacatccat actgatgccg taacccatta
     2341 tcagcatcag gatcaccgtt cccatcagcg ccagcgtcga acggaccgga tcgcggcgca
     2401 gttccagtgc ttcgcggcgg ctgtagctaa acagacggcg caggctaaat ccctgacgcg
     2461 gcgcgtgggt gatgtcgtgt accacgggcg gcgcttcggc ttcgttgctc tgccctgccg
     2521 cttcctgcaa ataggcgata aatgcctctt ccagactggc ggctccgcgt ttctcaacca
     2581 gttcctgcgg tgtaccgctg gcaagcactt ttccggcgtg catcagtgag atgcggtcgc
     2641 aacgttccgc ttcgttcata aagtgggtgg agatgaagat agtcactttg tcctggcgcg
     2701 agagatcgac catcaactgc cagaacatat ccctcgccac cggatcgacg ccagaagtag
     2761 gttcatcgag gattaacatc tccgggcgat gaatcaccgc caccgccagc gaaagccgct
     2821 ggcgaatgcc gagcggcaat gactccggca gaacatcttc aacgtcgttg agcttaaaac
     2881 gctcgctcat ttcagccact cgcgcgggaa tttccgcttc cgggatgtga aacaaacggg
     2941 catgtaactc aaggttttgc cgcacggtga gttcgttata gagcgaaaac gcctgcgaca
     3001 tatagcctac ccgacggcgg gtatcgatat cttttggatc aaccggttgc ccgaacagcc
     3061 acgcctcacc ttcgctggcg ggcagcagtc cggtaagcat tttcatggtg gtggatttac
     3121 cgcagccgtt cgaaccaagg aatccaaaaa tctccccgcg tggaatgcgg aaattgacgt
     3181 gatcaacggc aacgaaggaa ccaaaacgca tggtcagatc gcgcgcttcg atggcaatct
     3241 ctgcgttttc aggttgatac ggtgggatca ctaccgcctg atgcgcctgg cgttgtgctt
     3301 gcggtaacag atttataaat gcttcttcca gcgtggcgct ttgcgtttgc tgccgcaact
     3361 cttcggcgct gccggttgcc agcacttctc cggcattcat cgccaccaac cagtcgaagc
     3421 gttcggcctc ttccatatag gcggtggcaa ccagcacgct catattgctc tgccgctggc
     3481 gaatactgtc gatcagatcc cagaactggg cgcgggagag cgagtcaacc cccgttgttg
     3541 gctcatcaag aatcaacagt tccgggtcgt ggattaacgc acagcacagc cccagttttt
     3601 gcttcatccc gccggagagt ttccctgccg gacgatcgcg aaacggtgct aacccggtgc
     3661 tggtcagcag ctcattaatt cgcacttccc gctccgcttt gtcgtgaccg aacaggcgag
     3721 cgaaaaaatc gacgttttca tacaccgaca aggtgtggta gagatttttg cccagcccct
     3781 gcggcatcca ggcgatgcgc gggcagacgt cgcggcgatg cttcgggtcg cgcatatcgc
     3841 cgcccagcac catcacattg ccctgctcaa tgacgcgggc accggaaatc aacgacaata
     3901 agctcgactt cccgacgcca tccggaccaa tcagcccgac catacagcgg gccggaatat
     3961 cgagagtaat attgttcagc gcaacggttt ttccataatg ctggctcacg cccgccagtt
     4021 gcgcgacagg cgggacggga accagttcca gatgcgtcat tgcggcaacc tcaccacgag
     4081 gtcgtcaggc cacggaagtt cttcattcac ccgcacccac gctacgcccg gtaaaccggt
     4141 tttgacatat tccagatgct gctggagtaa ttccggtggg atacgcgctt tgacgcggaa
     4201 catcagtttc agccgttcat cgctggtttc gacggttttt ggcgtgaact gggcgacact
     4261 ggcgacaaaa ctgatggtcg ccggaatacg cagatctggc gcggcatcga ggatcagccg
     4321 ggcttcacca cccagtttca gcgtgcccgc ctgttcggtt ggcaggaaga aggtcatata
     4381 gacgtcgctg agatcgacca tattcagcac ccgaccgcct gccgccagca cttcccctgg
     4441 ctcggcaacc cgatactgca cgcgtccgtc acgcggggct ttcagttcgc tgtcatcgat
     4501 atctgcggca atgcgccgtt cagtggcttg tgccgcttcg acgcgggttt gcgcctgaat
     4561 gatattggta cgtgccgcct ctatagcggc tttagaagca gaaacctgag cttttgccga
     4621 ttccagcgta gctcgggcgc tctcagcggc ggcgcggtcg tcatccagct gttgcgcaga
     4681 aatagcccct cgttgggcca gtgaacggga acgcatatga cgttttgcta cggagtccag
     4741 ttctgcctgg cgttgattaa ccagcgactg cgcggcgcga ctttcgcttt gtcgttgctc
     4801 cagcaaagcc tgcgcggcgg caacggcgct ttgtgcctct ttgatttgcg cgatggcttc
     4861 cagtcgctgt tcctgcaaca cgcgagtatc catcttcgcc agcacttcac cttcgcgaac
     4921 aaactggcct tctttcacca gaatggtgtc gatacgcccg gcaattttgc tggcgatatc
     4981 cacttccgtc gcttcaatgc gcccattgct gacagcaaag ccttccggca cacccgccgg
     5041 gcgcaacagc caccacgcca cgacggcagc caccgccagt gccccgacaa cccaccacgc
     5101 cagatggcgc ttactcttat ccataatcgg cccgccataa tccctgtaag caaacgacgc
     5161 gtgttacaac tgccgccgaa cgacgttcag cggattcttc catccgggat aagaaggaaa
     5221 cgacaaatac gaccagcgcg cagagcattg agcgccaacg gggaaggctt agtgaaaacg
     5281 ggtagtaatg ttgtcatcat cagcattcct ggccgtaaat gaaacacgca tactcataag
     5341 tgtgatctat aaaactggct gacaaaacgg tatccatcct gttggatcgc gtttcacgcc
     5401 tgttcagttg caggaacaga gcattccttt ttacgtttta tcaacagtct caaccagata
     5461 gtaacattaa tattcatgtt gtctatggtt cagagctgta acattgtgcg ccttctaatc
     5521 aaaggcataa gtccatttct gtttttttca tgctggtaag acttccaggg agtggttatc
     5581 tcaggccctg caccagggtt aatttggcaa ttatgcatgc tgaacattct tccagcatgt
     5641 ttactgtcac gggcgttaag acctaaaact gtccccgttt cacctataca ttctcttcca
     5701 ccattaccgt tatgtgcaac aacttgattt atacctaaca tttgtgagca tgctaatagt
     5761 gcttcaagtt taggtctgta atctgtcgaa acagaaaaat aacgattaaa ggatcgacaa
     5821 gagatagccc ggctattttt aaactttttg ccattaatta cagcatggtg ctttttattg
     5881 ataattgccg ctaattcgac cggatcgaga gcattggtca tttcatcact agagactgtt
     5941 atcggtccta taatgtaacg ttttcgctga tcatcaaaag ttatcccatg atgatttgcc
     6001 attattttgt atttggggcc atagaaacat acaggataat cttttatccc agcgtaggta
     6061 tcctcatcaa gtggtttcat gcgcgtgaag ttctgcatat agttaccatt gcaattcgtt
     6121 tcatgatttc cagctaatac atgaacgttt tcattgatac tcattatgcg ttttaataaa
     6181 tcgatgataa atttatcgcc acagatatta ctaaatcgat cgccagtctg gtcgcccaga
     6241 aacgtacatg gcgtgactgc gtctttatta ataaccaaat aaggctgaag actttctaaa
     6301 acaatgtctc ttctgttcag gatactgatt attatattaa cattagtgtc aagttgctca
     6361 ttaaagttct cccttagcaa tacgttgaga acttcatctt ctatattaag caactggcta
     6421 aggaaatttt ctccttcagc catgattccc aaatgtcccg ttgcaaaaag cgcgtacaaa
     6481 actgcaccga ctgagccatc ggtatcgcca aaataggttg gtctggtact tgtgggatca
     6541 acaggtgaac aattggagaa ataggttctg tcagcgaact ggggttttat cattgtttcg
     6601 aatttaatat caaatacatt gtattgcgat attttccagt tccagatggg tttgtacgac
     6661 tcagtaactg caacatcatt agcatcatca tttactgtca tattattaag taaataaaca
     6721 atgctgcacc aattaatatc ggtattatca agatagcagc cgtaatttat actattaatt
     6781 gtcacaataa actgataatt attatcccta cgtagaatat gagcgcgcat gcccgatagc
     6841 atactatatt tttctgaatg attcgtttgt atggtaaatg aaattttgtc atctcgaata
     6901 gattgttgga aacgttctat ttgccaatca tcaatttttt ccacaacttc accgagcatt
     6961 gtcctggcaa tacttgatat atcatcacct ataccatttg caatataccc aaacagttct
     7021 ttagtgaagt tagtaacatg ctcttgcacc atatcatttg ttgcgattgt cgttgtactg
     7081 tcgttagtgc ctgccacagt tccaattttc atcttttacc tcatataatt caccgcgagg
     7141 aatgcatagt taacatttaa tcttatgaca tacacctcat gttcgatgga aaaataatta
     7201 tattttgcac aaatcattca gccttaaagc ataatatgaa atttcttatc tcatagcaga
     7261 gaaagtccct aaagattgta gaggcgtcat cagtacatac ctctacaatc gattaagaaa
     7321 acctatgaaa tactggcagg attaaaaagg cataccaccg tattcatgga gttttaatga
     7381 gattgaaaac aactaagccc tgaggaataa attattttgt tatttttatc aagtactttc
     7441 ttcgtggaat ttagccccat aacaccatta tcaaattgtt gattctgatc taccccgacc
     7501 ccatgatggc catatttttg tctaatgtat atgctgggat cttcacattt gagtgtctct
     7561 ccacatatga agatacgttc aggttttggt ctgaaatcag tagtgttatt aatataatca
     7621 gcatatacag ctgtgggttt cataatattg cttcggatta gatgtaatag aggatcattc
     7681 atatgttctt tatgtttttt attaaatata tttgctaatt cctcaggatc agtaggattt
     7741 ttcatctgtt taaatggaac ctgcaaagca ccgaggcaat aacatgcatt ttcttcatct
     7801 cttattattc cgtgatgact ggttaaaact ttcctttctg agtcataatg acaaacatcg
     7861 agcgttttaa ttaagttata cgtatcaccg gcagagagtt tatgatttgc tagtcgtgca
     7921 gtatagttac cattaaaatt gatttcatga tttccagcca gaacgacgac atttttatta
     7981 atacggctat ctttattact ctccatattt cgcatagaat ttagcaaagt aaggatatat
     8041 ttatcaccaa aaatagtact aaatctatcg cctgtatgat ccccaaggaa gatacaaggg
     8101 gttatcgcat cgtcctttaa aaaaacatgt ttgtgcaact cattcaatat catgtctctc
     8161 gtatttaata actcgtagag cactttatta tttttatgct tgtatgcaga tgaagccatg
     8221 ttctcgtgat ttaataattc acataataaa ctccaaccct tttccctgat gcctatatgc
     8281 cctgaaacga gcaatgcata aagtacagcg cctactcttc catctgtatc gccaaaataa
     8341 gccgtgttgg cattttgtgg gttaatcaca ccacattgtt tataaaagtc tattttagaa
     8401 aacacagatt taacatagcc agcaaactta tccttaggaa acgtctcttt agagacatcc
     8461 caacgccagt cagctttaaa attaccgcta agaagagtaa agaaattttc aggtatgata
     8521 gaaagcccat taactttata agttatgtct gatttatctc cagaggaatt aataaaataa
     8581 taaccgccct tacactcaac agtaacggta atgggagacg ataattttaa cacatggtta
     8641 ttgtctggta taattgtaaa gataaacgtg tcagtagtat tgtcggc
//
LOCUS       DAZFWL020000172         8663 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFWL020000172 DAZFWL020000000
VERSION     DAZFWL020000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.172"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..605
                     /locus_tag="ACHKNP_RS21940"
                     /old_locus_tag="pgaptmp_004388"
     CDS             <1..605
                     /locus_tag="ACHKNP_RS21940"
                     /old_locus_tag="pgaptmp_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HGU4706437.1"
                     /translation="PYIKDPIIRSYIHSGEDNKFDDFMRQHRFSKVDFDTQQMIHFIN
                     RFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRSDDVSPLFIEIEDN
                     LRTFNTNELTRKDNIFILFSSADDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHK
                     MLPINLTQETLFSSKSHGKYALFPIFTASWRATRIKNIGI"
     gene            828..1325
                     /gene="ubiC"
                     /locus_tag="ACHKNP_RS21945"
                     /old_locus_tag="pgaptmp_004389"
     CDS             828..1325
                     /gene="ubiC"
                     /locus_tag="ACHKNP_RS21945"
                     /old_locus_tag="pgaptmp_004389"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HGU4706438.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1338..2210
                     /gene="ubiA"
                     /locus_tag="ACHKNP_RS21950"
                     /old_locus_tag="pgaptmp_004390"
     CDS             1338..2210
                     /gene="ubiA"
                     /locus_tag="ACHKNP_RS21950"
                     /old_locus_tag="pgaptmp_004390"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HGU4706439.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2365..4788)
                     /gene="plsB"
                     /locus_tag="ACHKNP_RS21955"
                     /old_locus_tag="pgaptmp_004391"
     CDS             complement(2365..4788)
                     /gene="plsB"
                     /locus_tag="ACHKNP_RS21955"
                     /old_locus_tag="pgaptmp_004391"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HGU4706440.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4959..5327
                     /gene="dgkA"
                     /locus_tag="ACHKNP_RS21960"
                     /old_locus_tag="pgaptmp_004392"
     CDS             4959..5327
                     /gene="dgkA"
                     /locus_tag="ACHKNP_RS21960"
                     /old_locus_tag="pgaptmp_004392"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGU4706441.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5437..6045
                     /gene="lexA"
                     /locus_tag="ACHKNP_RS21965"
                     /old_locus_tag="pgaptmp_004393"
     CDS             5437..6045
                     /gene="lexA"
                     /locus_tag="ACHKNP_RS21965"
                     /old_locus_tag="pgaptmp_004393"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HGU4706442.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6118..7443
                     /gene="dinF"
                     /locus_tag="ACHKNP_RS21970"
                     /old_locus_tag="pgaptmp_004394"
     CDS             6118..7443
                     /gene="dinF"
                     /locus_tag="ACHKNP_RS21970"
                     /old_locus_tag="pgaptmp_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HGU4706443.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7559..7768
                     /locus_tag="ACHKNP_RS21975"
                     /old_locus_tag="pgaptmp_004395"
     CDS             7559..7768
                     /locus_tag="ACHKNP_RS21975"
                     /old_locus_tag="pgaptmp_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGU4706444.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7810..8325)
                     /gene="zur"
                     /locus_tag="ACHKNP_RS21980"
                     /old_locus_tag="pgaptmp_004396"
     CDS             complement(7810..8325)
                     /gene="zur"
                     /locus_tag="ACHKNP_RS21980"
                     /old_locus_tag="pgaptmp_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HGU4706445.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         2018 a         2068 c         2331 g         2246 t
ORIGIN      
        1 gtccttatat aaaagatcca attatacgca gttatatcca ttctggtgaa gataacaagt
       61 tcgatgattt tatgcgtcaa catcgattca gcaaggtgga tttcgatacc caacagatga
      121 tccattttat taacaggttt aatatgaata aagggctgat tgataaaaat aacaattttt
      181 ttatccaact tatcgatcag gccttacgat caacggatga tatgatcaaa gcaaatgcct
      241 ggtatcttta taaagagtgg attcgtagtg atgatgtttc acctctattt atagaaattg
      301 aagataattt aagaaccttt aacacgaatg aattaacacg aaaggataat atctttatcc
      361 tgttctcctc tgccgatgat gggccagtta tggtggtaag ctcccagcgc ttacatgata
      421 tgttgaatcc tacaaaagat accaattgga attccacgtg tatctataaa tccagacata
      481 agatgttgcc tattaatctt actcaggaaa cactttttag ctccaaatct catggtaaat
      541 atgcgctttt cccaattttt actgcgagtt ggcgagctac tcgtataaag aatataggta
      601 tttaagtaaa ggaaaacatc accgttcctg gcatcatggg cggtgatgcc cctacggttg
      661 ccctcgccag cacgggcatc ggcaaagcgt aaggttcaac agcggtttac cacttcatgc
      721 gattgttgcg tttttgttgc gcattagatc acttaatttg ctttacatct cccgtaaaca
      781 cttttctgcg atacaatgcc tttacgttat gtaacggaga gttcggcatg tcacaccccg
      841 cgttaacgca actgcgtgcg ctgcgctatt ttaaagagat ccctgccctg gatccgcaac
      901 tgctcgactg gctgttgctg gaggattcca tgacaaaacg ttttgaacag cagggaaaaa
      961 cggtaagcgt gacgatgatc cgcgaagggt ttgtcgagca gaatgaaatc cccgaagaac
     1021 tgccgctgct gccgaaagag tctcgttact ggttacgtga aattttgtta tgtgccgatg
     1081 gtgaaccgtg gcttgccggt cgtaccgtcg ttcctgtgtc aacgttaagc gggccggagc
     1141 tggcgttaca aaaattgggt aaaacgccgt taggacgcta tctgttcaca tcatcgacat
     1201 taacccggga ctttattgag ataggccgtg atgccggact gtgggggcga cgttcccgcc
     1261 tgcgattaag cggtaaaccg ctgttgctaa cagaactgtt tttaccggcg tcaccgttgt
     1321 actaagagga aaaaaatatg gagtggagtc tgacgcagaa taagctgctg gcgtttcatc
     1381 gcttaatgcg tacggataag ccaattggcg cgttactgct gctctggcca acattatggg
     1441 cgttgtgggt ggcgacaccg ggcgttcctc agctctggat cctggcggta tttgtcgcgg
     1501 gtgtctggct gatgcgcgct gctggatgtg tggtgaatga ttatgctgac cgaaagtttg
     1561 atggtcatgt taagcgcacg gcgaaccgac cacttcccag cggcgcggta acagagaaag
     1621 aggcgcgcgc actgtttgta gtgctggtac tgatttcgtt tttactggta ctgacgctga
     1681 atacgatgac cattctgttg tcgattgccg ctctggcgtt ggcgtgggtg tacccgttta
     1741 tgaagcggta tacccatctc ccgcaagtgg tgctgggcgc ggcgtttggc tggtcgattc
     1801 caatggcttt tgccgctgtg agtgagtcgg tgccattgag ttgctggtta atgttcctcg
     1861 ccaatattct ctgggcggtg gcttacgaca cgcagtatgc gatggttgac cgcgatgatg
     1921 atgtgaagat tggcattaaa tccacggcaa tcctgttcgg ccaatacgat aaattgatta
     1981 ttggtatttt gcagattggc gtactggcac tgatggcgat catcggtgag ttaaatggct
     2041 taggctgggg atattactgg tcaattgtgg tggctggcgc gctgtttgtt tatcaacaaa
     2101 aactgattgc caaccgcgag cgtgaagcct gctttaaagc atttatgaat aataactatg
     2161 ttggtctggt actattttta gggctggcaa tgagttactg gcatttctga tgatgtaaaa
     2221 aagccggatg atcatccggc tttcttctgg gttgcctgat gcgcggcgct tctcaggcct
     2281 acacaacaca tcgcaattta ttgaatttgc agattatgga aggccggata aggcgttttc
     2341 gccgcatccg gcaattctct ctgattaccc ttcgccctgc gtcgcactct caatcgtcaa
     2401 acgcacgtct gatgtaatca actccgccag caactgataa accttcatcg tttctgccgg
     2461 ttcggcatcg ccgctatcgc tgatataccc ttcatcacgc agtgtcagca ccagagaact
     2521 gaacaccgcc ttgtcgaaga actccggcgc gttgatgccg tgcagcacgg agagacgttg
     2581 cgcgacggtg cggctctctt tctccagcgt accgcggttg atcgacgggt tggcactcaa
     2641 caaccagaag gtgatggcat aacgttgcag cgtttcgcgc gcgcctgcgg cgagcagctg
     2701 tagcgtgcga gaatgcaccg ggttgatatg caactcatca tcttgcaggg taatcagccc
     2761 ctgacgttgc atctcatttg ccagcgcatc aataacgtcc ggcaactcgt cgcgatccca
     2821 gcgcaggaac agctccgctt tcagcattgg gtaaagcaca ttgacgtgct ccatcaatac
     2881 gtcgcgggag atgtggcgat gctgggtgac aattgccgcc atcagcgaag gcagcaccaa
     2941 catatgcgca atgttgttgc gatagtaggt catcagcacc gcttgctcgc gtggcagaat
     3001 gatgatgtcg ccgatagtgt ctttctcgac ttcaaacttg ttcatttgca gcgcgtgatc
     3061 gataagctcg ctggcgctgg ctgaaggaac ggtagagtcc gtggagtaag gtacgttgcg
     3121 catcagatcc aggtagcagt tgagttgctc ggttaactgc tcgcgggtga gtgagcgctg
     3181 acgtgatgcc agtagcgcag tacagcacag gttcatggcg tttgccgcgc ctgcgttgtt
     3241 aatgcgtacc atcagatcgg cagcaatatt attgaccgtc ggcgttaacc aggccggacg
     3301 caccgcttcg atgggatcga tagattcacg ccagtctggt acgtgctggt taaggtaggt
     3361 catcaacggc attggttcac cgaagttgac gtaaccctga ccgagattac gcagcttgct
     3421 taaaccgcgc agcatctgcg gcaggctctc tttctctttc gtcgcgccgc gcagttcttt
     3481 ggcgtaagta cccacttcca tgacgtgctc ataaccgata tagatcggaa tcagcgtaat
     3541 cggacgcgtg ccgccacgca gcatcgcctg aatggtcatc gacagcgtac cggttttcgg
     3601 atccagcaaa cgccccgtgc gggaacgacc gccttccacg aagtactcga cggaataacc
     3661 acggctgaac agttcgccga gatactcacg gaaaacggtg gaataaagtt tattgccttt
     3721 aaaagtacgg cgaataaaga acgcccccag acggcggaaa atcggcccgg ccggccagaa
     3781 gttcaggttg atcccggcgg cgatatgcgg cggcaccagc ccctggtggt acagcacgta
     3841 agaaagtagc aggtagtcca tgtgactgcg gtggcaaggc acatacacca gctcatgacc
     3901 gtcgtgggcc agctggcgaa cgcgctcggc gttatggacg ttgatgccct gataaagtcg
     3961 gttccaggtg aagcccagaa tgcggtcagt caggcggatc atctcgtaag agaaattcgc
     4021 cgcaatctct tccatcagcg caatcgcgtt ctgctgcgct ttttcatggg agattttttt
     4081 gctgcgcgct tcatcttcta ccgctttggc aatggcgcgg gaggcgagca gcttattaaa
     4141 cagatcctga cgagcaggca gacgtgggcc cacagcagcc agacgttgac gggcaaagtg
     4201 catacgcgcc acgcgcgcca gtttctgagc gatagtttta tctgtgccgt gttcatcggc
     4261 catgcggcgc agagaaactg acggcgagaa acgcacaaaa ctgtcgcgac cgagccacag
     4321 tacggcgaaa aatttttgta cgccgttaag catacgcagc ggcgggttta cttcgccttt
     4381 ttcacgcccc ggcgcgcgac caaacatcac cgatactggc accatctgca catccagatt
     4441 tgggttgctg cggtgcaaat caagatagtc gtggaacagc ttaatagact cttctttcgg
     4501 cgtgtaatag gtgaacacac gcggcccgcc gtgaatgaac acatagcgcg gcagtagcgt
     4561 gccgtcgatt tccagcggct ctaacgggtc aggcaagtca tgagccagac actgggcgcg
     4621 caacgtcagc aaatctgctt tcgagttgta cggtaaaacg tacataattg gacgagaggt
     4681 atccagcccc agttccgggg caggatctgc cggaatagac ttgcttttta ccaggatgct
     4741 taatggtaaa ttcagtaatt tgtagtaaat tcgtggccag ccggacataa acgatgtaaa
     4801 gcctctggtt aataatgcaa atgcgcggca aggatagcag aaagtcatgg gaaattctgt
     4861 ggtatccgct catgtttcgc gcggcgttac gcaaacccga atcatcggat ttaacggtac
     4921 actgatattg acgctcataa tgtaaaaagg ttctttcaat ggccaataat accactggat
     4981 tcacccgaat tatcaaagct gctggctatt cctggaaagg tttacgcgct gcatggatca
     5041 acgaagcggc attccgtcag gaaggcgtag cggtattgtt ggcggtggtc atcgcctgct
     5101 ggctggatgt ggacgcaatt acccgcgtgc tgcttatcag ctccgtgatg ctggtgatga
     5161 ttgtggaaat cctcaatagc gccatcgaag cagtggttga ccgaattggc tctgaatacc
     5221 atgagctttc cggacgcgca aaagatatgg gatccgccgc ggtgctgatt gccattatcg
     5281 tcgccgtgat tacctggtgc atcctgttat ggtcgcattt tggataaccc ttccagattt
     5341 cgataaatct ctggtttatt gtgcagttta tggttccaaa atcgcctttt gctgtatata
     5401 ctcacagcat aactgtatat acacccaggg ggcggaatga aagcgttaac ggccaggcaa
     5461 caagaggtgt ttgatctcat ccgtgatcac atcagccaga caggtatgcc gccgacgcgt
     5521 gcggaaatcg cgcagcgttt ggggttccgt tccccaaacg cggctgaaga acatctgaag
     5581 gcgctggcac gcaaaggcgt tattgaaatt gtttccggcg catcacgcgg gattcgtctg
     5641 ttgcaggaag aggaagaagg gttgccgctg gtaggtcgtg tggctgccgg tgaaccgctt
     5701 ctggcgcaac agcatattga aggtcattat caggtcgatc cttccttgtt caagccgaat
     5761 gctgatttcc tgctgcgcgt cagcgggatg tcgatgaaag atatcggcat tatggatggt
     5821 gacttgctgg cagtgcataa aactcaggat gtacgtaacg gtcaggtcgt tgtcgcacgt
     5881 attgatgacg aagttaccgt taagcgcctg aaaaaacagg gcaataaagt cgaactgttg
     5941 ccagaaaata gcgagtttaa accaattgtc gttgaccttc gtcagcagag cttcaccatt
     6001 gaagggctgg cggttggggt tattcgcaac ggcgactggc tgtaacatat ctctgagacc
     6061 gcgatgccgc caggcgtcgc ggtttgtttt tcatctctct tcatcaggct tgtctgcatg
     6121 gcattcctca cttcatctga taaagcactc tggcatctcg ccttacccat gattttctcc
     6181 aatatcaccg ttccgttgct gggactggtc gatacggcgg taattggtca tcttgatagc
     6241 ccggtttatt tgggcggcgt ggcggttggt gcaacggcga ccagctttct ctttatgctg
     6301 ttgctgtttt tacgcatgag caccaccggg ctgactgcgc aggcttatgg tgccaaaaat
     6361 cctcaggcat tagcccgtgc gctggtgcaa ccgttgctgt tggcgttggg ggctggggcg
     6421 ttaattgcgc tgctgcgtac accgattatc gatctggcgc tgcatattgt tggcggtagt
     6481 gaagcggtcc tggaacaggc gcggcgcttt cttgaaatcc gctggttaag cgctccggcg
     6541 tcgctggcga atctggtatt actcggttgg ttactcggcg tgcaatatgc ccgtgcgcca
     6601 gtaattttgt tagtggtcgg caatatcctc aacattgtgc tggatgtctg gctggtgatg
     6661 gggctgcata tgaacgtgca gggcgcggcg ctggcgacgg ttattgcgga atatgcaaca
     6721 ttgctgattg gtctgctaat ggtgcgtaaa atcctcaaac tacgcggaat ttccggcgaa
     6781 atgctgaaaa ctgcctggcg aggaaacttc cgtcgcttgc tggcgcttaa ccgcgatatc
     6841 atgctgcgtt cgctgttgtt gcaactctgt ttcggcgcga tcaccgtact tggtgcgcga
     6901 ctggggagtg acattgtcgc tgttaacgcg gttctgatga cgctactcac ctttaccgcc
     6961 tatgcgcttg atggttttgc ctacgcggtt gaagcgcact ccggtcaggc atacggtgcg
     7021 cgcgacggta gccagttgct ggatgtatgg cgggcagcgt gccgccagtc ggggattgta
     7081 gcgttactgt tttcggtggt ttatttgctg gcaggggaac acatcattgc gttgctgacg
     7141 tcgttaaccc agattcagca gctggctgac cgctatctta tctggcaggt gattttgccg
     7201 ttggttggcg tctggtgtta tctgctggac ggcatgttta taggcgcaac gcgtgccgcc
     7261 gaaatgcgta acagtatggc ggtggccgcc gcaggttttg cgctgacgct ccttacgctg
     7321 ccgtggctgg gtaatcatgg tttgtggctg gcattaaccg tctttctggc gttgcgcggg
     7381 ctttctctgg cggctatctg gcggcgtcac tggcgcaacg gtacctggtt tgccgcaacg
     7441 tgacggttaa aaattctgaa taaataatcc taagccaaat tgctgactac acttaatctc
     7501 acgttcagaa gaaaagtgaa cgtactctca ttcacaacct aacgatgagg tcttgattat
     7561 gaataaagat gaagccggcg gtaactggaa acagtttaaa ggtaaagtga aagagcaatg
     7621 gggcaaactg accgatgatg atatgacgat cattgaaggt aaacgtgatc aactggtcgg
     7681 taaaatccag gaacgttatg gttatcagaa agatcaggca gaaaaagagg tcgtggattg
     7741 ggaaacccgc aatgaatatc gctggtaatt aatccctcct gcccgacgtg tacaaggatg
     7801 tacgccctct taacgcggtt tctttttcac ctgcacagag tgatcatgct ggcactgttc
     7861 aggatgacga cacgcttcca cttccacgca tgccgcacag agcccatgtg cttcaatcac
     7921 attatgccgc agggcaaacc ccatttttgc cgccagcgta tgcataatgt cttccacgcc
     7981 ttctgcacac tcttctttca ccgcgccgca gcgatcgcaa ataaacatgg ctgacgtatg
     8041 ggtgggctgg tcgaacagat gacagagcac ataactgttg gtggattcca ccttatgcac
     8101 aaaaccttgc tcaagcagaa aatccagcgc gcgataaacc gttggcggct tggcttgcgg
     8161 ttcagcttcg cgcagtaaat caagcagatc ataagcgctg atagcgccat cctgcagact
     8221 catcaggcgc aacacttcca ggcgctgtgg ggtcaggcgc acattacgct gcgcgcagat
     8281 tttttcagcc tgcgctaata actcctgcgt tgtggtcttt tccatcgggc acctcaaagt
     8341 gggggttaag aatgtcctta ctttaccatg ttccaggaaa aacaccgaga acccttattg
     8401 ttgccgtaat gttgattttc tgttttgtag gtaaggtgtt gtgttgcctt gtcgtaccat
     8461 tatcaacacg ataataatta ataattcatt tttaaataaa ttgcttcaag ttaactcgct
     8521 atttatttaa ctgggtttaa acgcatacct gtagttcaga aattatgagc gatttttgat
     8581 agtgaccaga tggcaaaaga attataagga ggacacggta cgacaaaaaa ggtagatgaa
     8641 tcatgttgaa gataataccc ggt
//
LOCUS       DAZFWL020000173         8534 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFWL020000173 DAZFWL020000000
VERSION     DAZFWL020000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.173"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..274)
                     /gene="cas2e"
                     /locus_tag="ACHKNP_RS21985"
                     /old_locus_tag="pgaptmp_004397"
     CDS             complement(<1..274)
                     /gene="cas2e"
                     /locus_tag="ACHKNP_RS21985"
                     /old_locus_tag="pgaptmp_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HGU4706446.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSF"
     gene            complement(274..1194)
                     /gene="cas1e"
                     /locus_tag="ACHKNP_RS21990"
                     /old_locus_tag="pgaptmp_004398"
     CDS             complement(274..1194)
                     /gene="cas1e"
                     /locus_tag="ACHKNP_RS21990"
                     /old_locus_tag="pgaptmp_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HGU4706447.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(1191..1841)
                     /gene="cas6e"
                     /locus_tag="ACHKNP_RS21995"
                     /old_locus_tag="pgaptmp_004399"
     CDS             complement(1191..1841)
                     /gene="cas6e"
                     /locus_tag="ACHKNP_RS21995"
                     /old_locus_tag="pgaptmp_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HGU4706448.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGPAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(1823..2569)
                     /gene="cas5e"
                     /locus_tag="ACHKNP_RS22000"
                     /old_locus_tag="pgaptmp_004400"
     CDS             complement(1823..2569)
                     /gene="cas5e"
                     /locus_tag="ACHKNP_RS22000"
                     /old_locus_tag="pgaptmp_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HGU4706449.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(2580..3635)
                     /gene="cas7e"
                     /locus_tag="ACHKNP_RS22005"
                     /old_locus_tag="pgaptmp_004401"
     CDS             complement(2580..3635)
                     /gene="cas7e"
                     /locus_tag="ACHKNP_RS22005"
                     /old_locus_tag="pgaptmp_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HGU4706450.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWSAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(3647..4186)
                     /gene="casB"
                     /locus_tag="ACHKNP_RS22010"
                     /old_locus_tag="pgaptmp_004402"
                     /gene_synonym="cse2"
     CDS             complement(3647..4186)
                     /gene="casB"
                     /locus_tag="ACHKNP_RS22010"
                     /old_locus_tag="pgaptmp_004402"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HGU4706451.1"
                     /translation="MSIVKEEHKATLRKWREELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSKGRFTRL
                     SAVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQNQ"
     gene            complement(4183..5745)
                     /gene="casA"
                     /locus_tag="ACHKNP_RS22015"
                     /old_locus_tag="pgaptmp_004403"
                     /gene_synonym="cse1"
     CDS             complement(4183..5745)
                     /gene="casA"
                     /locus_tag="ACHKNP_RS22015"
                     /old_locus_tag="pgaptmp_004403"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HGU4706452.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKNHGRWDDIWQDGLEAEKLREALLSLEHAFQFGADSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(5843..>8534)
                     /gene="cas3"
                     /locus_tag="ACHKNP_RS22020"
                     /old_locus_tag="pgaptmp_004404"
     CDS             complement(5843..>8534)
                     /gene="cas3"
                     /locus_tag="ACHKNP_RS22020"
                     /old_locus_tag="pgaptmp_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HGU4706453.1"
                     /translation="YPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAA
                     MGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELK
                     IQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHH
                     GQPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYIS
                     ATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGINTL
                     FPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLF
                     FGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSE
                     EPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILL
                     ADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEA
                     PFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKC
                     IAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCSL
                     QRASQVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKD
                     ERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHA
                     ARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPE
                     KLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGE
                     AFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         2042 a         2367 c         2024 g         2101 t
ORIGIN      
        1 gaaaagaaac caaacgtaac ccatccaaat ccaccggaat acgtctgttt tctccccagg
       61 tctgaaattc aaaacccgac tcggtattgg tcgcccaggc catcaccaca tttccgcaac
      121 cagccagttg ggtaatttgc tgccagatca tctcccgaat acgttttgat gtatcaccaa
      181 catacacacc ggcacgcact tccagtagcc agattgcgag ccgtccacgt aagcgcggcg
      241 ggacattttc tgtaacaacc acgaccatgc tcatcagccg cgtcctcgat gaccgctatc
      301 gcccaacgtt tcaggttcgg ggatggctgg cggtaacata tccggcgcag gttgtggcgg
      361 ttcaatttca cctgcggcaa ggacttcctc aattaacggt attaatttgc ccgttaactt
      421 agtgctacgg aaaatatcgc gacaggctaa tctgacttct ttatcaggtt ctgcgggttg
      481 cctcgctgct atttcaaatg cctttggcac aaccgaatca aatttaatga tatcggctat
      541 gtcataaaca aatgaaagcg gtttgccact atggataaac ccaatagcgg gcgcatatcc
      601 cgcggctaat actgccgctt cagatatacc gtacagacat gatgtagcag cactgatgca
      661 gcgattcaca acatcgcctt tttcccagtc tttaggatcg tatttgcgac cattccattt
      721 cacaccatat tgtttcgcca gtaatgcata cgtctggcga acgcgagatc cctcaattcc
      781 acgtaactgc tccactgaac ggcgagctgg ctgtggttca cgaaaacgta attcatacat
      841 tttgcgcacc accttcaacc gtagatcttc cgttaaagcc agctttgcct ggtagagtaa
      901 tttatctgcc cgcgccccac cgggttgtcc ggaggagtaa acgcgaacgc ccgcttcacc
      961 gacccagacc agcagtgttc ccaccgtggc ggccagatgc accgccgcgt gggaaactct
     1021 cgttcctggt tcgagcataa tgcaggcgac cgatcccacc ggaatatgcg ttcggatccc
     1081 tgttttgtcg atcagcacga aagcgccgtc cagtacgtcg atctgaccgt actggaggaa
     1141 gatcatggag gtgcgatctt ttaatgggat cgggctcagt ggtacaaacg tcacgcctct
     1201 gctccgggtt tgatcagcat cagaccacag ccgaacgccc ggcttttacc atacccctgg
     1261 ctcaggcgtt gcacaaataa cccaggatcg gtgaccgtaa gcatccccgt ataatccacg
     1321 ctactgaact ggatcagttg ccgggagttt tcccgccgca gttgctgttg tcggtaggca
     1381 tcaaccgagg tatccagcag tgtaaaaccg cttctctccc cctgcgctgc cagccagtcc
     1441 agcgccgcct gttgttgatg caaccagaca tcacttcctt ccgccggccc cctcacctgc
     1501 cgtttcgcct ccatcagcag atcgtggcgc ttgcccgctt tacagattgt tggattcgcc
     1561 cgcaggttga aacaaagttg ttgtccggta cacagctcgg gcgcaaatga ccggcattct
     1621 atggtgaacg tatcactttc cgccgggcgt tcctgcgaca gtacaaaaaa gcgaaacgcg
     1681 ccctggagtt cttcccgacg ataaagaaat tgcctttctt tgccgccagg gaagagatcc
     1741 cacagccact gatgcatcac atattcaccg cgatccacca gatgcagcaa ctgcgcaggc
     1801 gaaagctggc cggtatgtaa ggttattctt gagaggtaca tggttcctcc ttgctgagcc
     1861 acggcccctg attgacggtg cgttccccaa acagccactg ctgacgattt aaaggaacat
     1921 cccggcggcg taatattttg ctcgcgacca ggccgtcgtg tttcccttcc caccagcatt
     1981 catcctgaag tttcgggagt gagactttca gtttatggaa atgatcctga tactgttggt
     2041 atgcattaca taaggcatca cacgcgttgc cttcgagcag taacggcgca agaggtaatg
     2101 ccagcggatg actttttcgc cccagataaa gcggaaaaac tggatggcgt aaaccgtcct
     2161 gcaactgttc gaggctgtaa ggcgcatcgg ctgttgttgc caccgccacc atccaccagg
     2221 catcggtgta gtagtcgcgc cgggagataa tcgcgctcaa aagctcaggg tcgctcaact
     2281 cttcgcgacg gctgaaataa cgtgctttac gcacctcttt tggcatctgg accgtgtgat
     2341 aatcccgtgc ccagcgcggg ttacggctgg cgcaaaccac cagtgaatag tggcgattaa
     2401 acgcgtttaa gcgttcggta tcatcacgcc gaatccctac cccggcagcc agtagcccca
     2461 gcaatgctga gcgcgaagga agttcatggg tatgacgaac ttcacctgga gcatcgaccc
     2521 cccaggacgc catcggccca tgaagctgaa aaatcagata ttggctcata agccgcccct
     2581 tacgcgcaga taaagtccag cacgtccttc atgcttccct gtttgttcat cacgtcaaaa
     2641 cttgcgcatt cggtcttctg ttcgtagacc gtattcatgt tttcgcgaag cgttgtaata
     2701 cgctgcaccg ccacatctaa ctgacgtgtg ccattaatgg gttcatagaa agccgccgcc
     2761 agagagcgtg gttgatcggt gcctttttct gccagcgccc aggaggcgta ggcacggctg
     2821 gcaaagctgt tctgtttgcc ggttggggag actttaagtg cggcttccgt aaaggcacgt
     2881 atggtctgat tagctaacgc ttcgtcaccg ccaaggtttt cgaccagcag atctttatca
     2941 atgcagatat aggtgtagaa cagtgcagaa ccgaatccgg tttcaccaag atgcccggca
     3001 ccggcatctt cagaagcctg gcgcaaatca tcaacggcgg tgaaaaaatc atcttcgaca
     3061 atcgtttcac tgacaccaaa tgcgtgcgct acctggcagg cggcttcaac attaaactcg
     3121 ggtttattcg ccagcatacg accaaacatg gcaatatcta ccgccatacg gtctttgcgt
     3181 aacaacgcga gatcttcctc ttttggcgcg cgcttttctt ctgcaagctg atgggccagc
     3241 gcttttacgg cgtcaaattc tgccgggctg atatggacta actgttcagt ttcggcgtta
     3301 gtgagcggat cttttggttt tttgtcattt ttagctttcc caagataatc agcaattttt
     3361 gccgaccatt cgatggcttt tttatcttca atccctttct caatcaggat agttgccgcc
     3421 tcacgcgcaa tacgcccact gcgaatacca atatggcccg ccagtgcctg ttcaaaaagt
     3481 gcagaagtgc gccacgcacg tttcagactt tgcgaggaaa cgcgcagacg cgttgctcca
     3541 cccagcacca cagtttttgg cgcaccggta tcatcacggt taaggttggc agcagggtaa
     3601 gcggttaaca aatgaagctg aataaatgtt gtcatgagat agtcctttat tgattttgtt
     3661 cgttatcggc gtcgccagcc tggtaatatt ccagcgccca gcggatacgg ataaattcgg
     3721 ttggccgctg ctggcggcgg tgatgattga gcaggtcgtc gctctcctgg caccaacgga
     3781 agaccccctc tgccagagag tcaagattga cggaaccgtt taataatctg actgcgcggc
     3841 gtaactggcg cagtaattca tccggtgttt ttactgccga cagacgagta aaacgcccct
     3901 ttgacattac cgccgccagc tgcgcggcaa aaggctgccg ttcatcaata gcttttacat
     3961 tggcgctaac tgcggcgacc agcgccagtg cggtaaaacg ccactcttgt gcttcaattt
     4021 tccacaaagt gtgggtctgc atcagcagag aacgaaaccc ttctgacaag caaacatcat
     4081 taaccgttgt gctacgccgc agacttgcac gattgccgcg tttagcctgc aactcttcgc
     4141 gccatttacg cagcgtggct ttgtgctcct cttttacaat actcattcag cagcctcctg
     4201 ctttttctcc ctggcggctt tagcactttg cttctccgcc gatgttgtga aatatttctt
     4261 gcgcgcggtc ataacgcgtt tcaaatcaac aggctcatat ggattggtga atacacgctc
     4321 gtcaaaatcc tgacgcgcga ataaccaaat ttccttttgc catttgccga gtaattcgtc
     4381 cgcatcctga ccttcttcaa tttggcgcac taacctcagg aagcgatgct gagttttgtt
     4441 ccagaagtcg atatccacaa aactgaaatc accccgtgca ccttttggat cggagaacca
     4501 cgcttctttc aatgcacttc gtaacagact tagaatccgt gaagccgttt gcgcagccag
     4561 acgcagtttc ggtatctggc cttctttttt cttgagcagc agcgggaaat ggtgttcgta
     4621 ccaacagcgc gctttcatgt tgtcgaaatc atagccaaat ccccacaggc ctacttttgc
     4681 ctgtttcaga ctgctggcat taaagagttt caccaccagc gcgggaagtt ccgtattgtt
     4741 ttctgattta cccgtttcga taaggcctaa ccagtcgcgc cagattaaac cgcccggttg
     4801 tggtttgacc gagtaaaact cgccaccctc tttaagtggt atacggtaag gcgttaaggg
     4861 atgctgccac atggcataat tcgcaccgta atttttggtc gtcatcaggc tcaggagcgc
     4921 gtcgctctgt tcaccgcaaa tatcgcagtt gccgactgtc gtggtattaa aatcaatacg
     4981 aatacggcgc ggcattcccc agtaagcctg gagtttattg acctgatcat gggttaccac
     5041 cgcaccggcc agttcgctgg ttcgcgtcgg gccaagccag gggaaaacca gatcgtcaaa
     5101 ttttttgggt agcggtaagt cggcttcatc ctgcggcatc acattgggcc acagtttgcg
     5161 ccacaagggc gtttgttgat tgccttgata ttcctgtaat tcaatcagag tggtcatcgg
     5221 cccaccgccg cgcaaaccgg tgcgatagcc tttgccgcct gatggcgcat ttaactgtag
     5281 ggagaacaga gctaacgcgg aacaatgagg gcatacgtgt tcagtcacgc cacgcttaat
     5341 aaagtggtct ttattaaact tcgttgtttg agcgccggga atctcaggca gtagcgaagc
     5401 gacctgaact ttatctccct tgagcgcctc gaaatcctgc ataaatgaag gggaatctgc
     5461 cccaaactgg aaagcgtgtt ctaaggacag caatgcttcc cgtagctttt cagcctccag
     5521 tccgtcttgc cagatatcat cccaacgacc gtgatttttg ggcgcgaagc tggtttgtag
     5581 tagccccagc aaaaactgcc aggccgcccc ctggagatct gcccgcggcg cagcgatatc
     5641 gacaacattt tcatcagcca gatcgactgg tgccagcttg cctgtcgttc cgtctttaaa
     5701 acgaacaggc aaccacgggg ttgtcagaag tgaaaacgag ttcatacgca tcctctcgta
     5761 aaaaagacca tcccaggtcg gttgtccatc tttccatcct tttcggtgta tcaactattc
     5821 agagagtcct gtcgcagaca tctcaaccaa ccttgccaat caatccttcc gtagccgaat
     5881 acccgcaact ttcgtcatcg ttgactaaaa tcacgtttgc catctccgga tcttgtcgct
     5941 gttcaatgca ccacagcctg aacgcctctc cctccagtaa agaaaactca tcccgatgtt
     6001 ttttccacca gcttcggcgc actctgacaa cgctcatttc ccatgcgtga gcaccggtgg
     6061 cataaggctt caccacaccg tcaatacagg tagccagcca cagggaaaca gactcctcag
     6121 ccagacgtgt cgacagtttt tccgggaggt aatcgttgat attggcggca tagccgggcc
     6181 tgaagttcag cacaaacttt ttagccattg cgcgatcgca gtaatatttg cccacttgct
     6241 cctgctcgct gcgggcaaat ccttccggca ttaccacgtc ctcaccgtag acggattcaa
     6301 taagaagccg ggctgcgtgt ggcatctgaa tagcaccttg ctcacgcagc acacgctgcg
     6361 tcagccagat tcgcccatga tcgggataga caaatgcact gttacgcatg gcactgccaa
     6421 accattcgtc gccaggagag tcgtcccaga cgggagccag aatcagcaat tcaggggggg
     6481 aacgctcgtc ctttccgtca cgctttaact gaccattaat atcgcggata tgccgctgta
     6541 atcgtcctgc tcgctgaatc agcaaatcaa caggggccag gtcggagatc atttcgtcca
     6601 ggtcaatatc cacgctttgc tcaattactt gtgtacaaac aatgacttgc gacgctctct
     6661 gtaaactaca atatttacca aagcgtgcca gcgtttccgt ttcaattcgc tggcgatcgc
     6721 taaaagcaaa gcggctatga aaaagtgaaa ggctggaagc aggaatgacg ccgcgggcaa
     6781 gcagctgacg atgaacctta atagcgtcat cgacagaatt ccggatccag gcgatgcatt
     6841 tcccctgact taccgccgat tcgatacgcg caatacactc ttgttcacta tgaagccaac
     6901 ccacgctgac gctacgctca acatctttgc gcgtcgctac ccggtgtgag ttcacatcgg
     6961 atttcgtgac atgcgtcagc caggggtaat catccttttc caggaacgga gcttcttgct
     7021 ggccctctgt accccgcgca aaggcagcga cgagtttgtc gcgctgctgt tgagataacg
     7081 tggcagaaag caaaataacg ctgtttccgc cacgcgcctg ccgctcgatc aacccttcaa
     7141 gaatgcacga catgtaagcg tcacaggcgt ggatctcatc agccagcagg attttgttac
     7201 tcagccccag aagccgcaga ttattatgtt tgaacggcat caccgccatc atcgcctgat
     7261 ccagcgtgcc gacgccaatt tcagccagca acgccttctt gttactgtcg gcaaaccagg
     7321 ccgcacatcc ctgactgaac gtttgttcat ccggttcttc tgacccgact aaatcaccag
     7381 accagagtga ttcattaaag cggtccatta atgtgcgggc actatgtgcc agcaccaggc
     7441 tggggcggga ttctggcgaa tagaaagcaa gccaggtttt gaccagccga tcgtacatgg
     7501 cattggccgt tgccattgtt ggcagaccaa aaaacagccc ctgagctttc cccgcagcta
     7561 tcaacctgtg cgccaggata agcgccgctt ctgttttacc tgcgccagtc acatcttcca
     7621 gaataaataa ctgcggtcct tgctggctga tatccagatc cagcaccttt tgctgtaacg
     7681 gtgtcggatt ctcaataaaa ggaaacagcg tattaattcc gttgaaaggc gcgacttttg
     7741 cttttagagg aaagacggtt aaggcgtttt gagcctgaat taaagttttc tgccagtaac
     7801 ctttaatatc cattggatgt gcgacgcgtg gaaaaaatcg cgttgacgaa cccgtccagt
     7861 ctgcgagcac gacggttgca gagatatacc aggaaaggtg ttttataagt tcaatgccct
     7921 catcatcatc ccagaatgcg gggatctcta tgagcggaaa cagtggtttg atttcaagga
     7981 gaaaatctcg tgctgcagct ttgtcttcag gcagaaaatt atccaattca tccatacgat
     8041 cagggggctg accatgatgc cctgtcgtca cgggcatcca catttctatc actcgcctaa
     8101 gtttgcgcgg agagagcgaa gatgaaggaa gtaactcctg acattcactt aaataatgat
     8161 tccacaacca gtagcccagc gttgaatgag agattttttc gtaatttttt ctggcacctt
     8221 cctgaatctt gagttcaggg ggcaggtaaa gttgttgaaa agagcgggca aattttccaa
     8281 tatcgtgcca gcacagcaac caggcgaaaa attgagccgc ctgttccttg tcagaaatcc
     8341 ctaattgacg aaaataatca gccagcccga agcaatttct tttaaccatt aaatagccca
     8401 tcgcggctac atccagcgaa tgccagcaaa gaaggtgata gtcgtcgccc ccgtctttct
     8461 cgccgcgtcg ggtttttccc cagaaatcaa aaaaagtcac aatattttta tccttcagta
     8521 aacttaaagg atat
//
LOCUS       DAZFWL020000174         8466 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFWL020000174 DAZFWL020000000
VERSION     DAZFWL020000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.174"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            135..2513
                     /gene="ppsA"
                     /locus_tag="ACHKNP_RS22025"
                     /old_locus_tag="pgaptmp_004405"
     CDS             135..2513
                     /gene="ppsA"
                     /locus_tag="ACHKNP_RS22025"
                     /old_locus_tag="pgaptmp_004405"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HGU4706454.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2570..4216)
                     /gene="fadK"
                     /locus_tag="ACHKNP_RS22030"
                     /old_locus_tag="pgaptmp_004406"
     CDS             complement(2570..4216)
                     /gene="fadK"
                     /locus_tag="ACHKNP_RS22030"
                     /old_locus_tag="pgaptmp_004406"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HGU4706455.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4273..4566)
                     /locus_tag="ACHKNP_RS22035"
                     /old_locus_tag="pgaptmp_004407"
     CDS             complement(4273..4566)
                     /locus_tag="ACHKNP_RS22035"
                     /old_locus_tag="pgaptmp_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU4706456.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4563..5852)
                     /locus_tag="ACHKNP_RS22040"
                     /old_locus_tag="pgaptmp_004408"
     CDS             complement(4563..5852)
                     /locus_tag="ACHKNP_RS22040"
                     /old_locus_tag="pgaptmp_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4706457.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCIMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5908..6846)
                     /locus_tag="ACHKNP_RS22045"
                     /old_locus_tag="pgaptmp_004409"
     CDS             complement(5908..6846)
                     /locus_tag="ACHKNP_RS22045"
                     /old_locus_tag="pgaptmp_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HGU4706458.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6866..7630)
                     /locus_tag="ACHKNP_RS22050"
                     /old_locus_tag="pgaptmp_004410"
     CDS             complement(6866..7630)
                     /locus_tag="ACHKNP_RS22050"
                     /old_locus_tag="pgaptmp_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HGU4706459.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            7946..>8466
                     /locus_tag="ACHKNP_RS22055"
                     /old_locus_tag="pgaptmp_004411"
     CDS             7946..>8466
                     /locus_tag="ACHKNP_RS22055"
                     /old_locus_tag="pgaptmp_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGU4706460.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQG"
BASE COUNT         2022 a         2186 c         2191 g         2067 t
ORIGIN      
        1 ggatcagtct taaaaagtaa aaaaatatat ttgcttgaac gattcaccgt ttttttcatc
       61 cggttaaata tgcaaagata aatgcgcaga aatgtgtttc tcaaaccgtt catttatcac
      121 aaaaggattg ttcgatgtcc aacaatggct cgtcaccgct ggtgctttgg tataaccaac
      181 tcggcatgaa tgatgtagac agggttgggg gcaaaaatgc ctccctgggt gaaatgatta
      241 ctaacctttc cggaatgggt gtttccgttc cgaatggttt cgccacaacc gccgacgcgt
      301 ttaaccagtt tctggaccaa agcggcgtaa accagcgcat ttatgaactg ctggataaaa
      361 cggatattga cgatgttacc cagcttgcga aagcgggcgc gcaaatccgc cagtggatta
      421 tcgacactcc cttccagcct gagctggaaa atgccatccg cgaagcttat gcacagcttt
      481 ccgccgatga cgaaaacgcc tcttttgcgg tgcgctcctc cgctactgca gaagatatgc
      541 cggacgcttc ctttgccggt cagcaggaaa ccttcctcaa cgttcagggt tttgacgccg
      601 ttctcgtggc agtgaaacat gtatttgctt ctctgtttaa cgatcgcgcc atctcttatc
      661 gtgtgcacca gggttacgat caccgtggcg tggcgctctc cgccggtgtt caacggatgg
      721 tgcgctctga cctcgcatct tctggcgtga tgttctccat tgataccgaa tccggctttg
      781 accaggtggt gtttatcact tccgcatggg gccttggtga aatggtcgtg cagggcgcgg
      841 ttaacccgga tgagttttac gtccataaac cgacacttgc agcgaatcgc ccggctatcg
      901 tgcgtcgcac catggggtcg aaaaaaatcc gcatggttta cgcgccgacc caggagcacg
      961 gcaagcaggt taaaatcgaa gacgtaccgc aggaacagcg tgacatcttc tcgctgacca
     1021 acgaagaagt gcaggaactg gcaaaacagg ccgtacaaat tgagaaacac tacggtcgcc
     1081 cgatggatat tgagtgggcg aaagatggcc acaccggcaa actgttcatt gtgcaggcgc
     1141 gtccggaaac cgtgcgctca cgcggtcagg tcatggagcg ttatacgctg cattcacagg
     1201 gtaagattat cgccgaaggc cgtgctatcg gtcatcgcat cggcgcgggt ccggtgaaag
     1261 tcatccatga catcagcgaa atgaaccgca tcgaacctgg cgacgtgctg gtcactgaca
     1321 tgaccgaccc ggactgggaa ccgatcatga agaaagcatc tgccatcgtc accaaccgtg
     1381 gcggtcgtac ctgtcacgcg gcgatcatcg ctcgtgaact gggcattccg gcggtagtgg
     1441 gctgtggtga tgcaacagaa cggatgaaag acggtgagaa cgtcactgtt tcttgtgccg
     1501 aaggtgatac cggttacgtc tatgcggagt tgctggaatt tagcgtgaaa agctccagcg
     1561 tagaaacgat gccggatctg ccgttgaaag tgatgatgaa cgtcggtaac ccggaccgtg
     1621 ctttcgactt cgcctgcctg ccgaacgaag gcgtgggcct tgcgcgtctg gaatttatca
     1681 tcaaccgtat gattggcgtc cacccacgcg cactgcttga gtttgacgat caggaaccgc
     1741 agttgcaaaa cgaaatccgc gagatgatga aaggttttga ttctccgcgt gaattttacg
     1801 ttggtcgtct gactgaaggg atcgcgacgc tgggtgccgc gttttatccg aagcgcgtca
     1861 ttgtccgtct ctctgatttt aaatcgaacg aatatgccaa cctggtcggt ggtgagcgtt
     1921 acgagccaga tgaagagaac ccgatgctcg gcttccgtgg cgcgggccgc tatgtttccg
     1981 acagcttccg cgactgcttc gcgctggagt gtgaagcagt gaaacgagtg cgcaacgaca
     2041 tggggctgac caacgttgag atcatgatcc cgttcgtgcg taccgtagat caggcgaaag
     2101 cggtggttga agaactggcg cgtcaggggc tgaaacgtgg cgagaacggg ctgaaaatca
     2161 tcatgatgtg tgaaattccg tccaacgcct tgctggcaga gcagttcctc gaatatttcg
     2221 acggcttctc aattggctca aacgatatga cacagctggc gctcggtctg gatcgtgact
     2281 ccggcgtggt gtctgaattg ttcgatgagc gcaacgatgc ggtgaaagca ttgctgtcga
     2341 tggctatccg tgccgcgaag aaacagggca aatatgtcgg gatttgcggt cagggtccgt
     2401 ccgaccatga agactttgcc gcatggttga tggaagaggg gatcgatagc ctgtctctga
     2461 acccggacac cgtggtgcaa acctggttaa gcctggctga actgaagaaa taaaataaat
     2521 ccccggcggc gttcagtcgc cgggtttatg tgatccccga agataaaact tattcaatct
     2581 cttcacagac atcctgcgtt aatcgccgca taatatcttt tcttaacaaa aacttttgta
     2641 ttttacctga ggcagtgcgc ggtagttttt cgattaccac gatatgttca ggatatttat
     2701 attttgcgac ccgtttacgg ctaaaaaaag ccaccacctc ttccagcgat aatgaatgat
     2761 gcggcgcttt cagcacgaca taagcgcatg atcgttcacc taaacgttca tcgggcattg
     2821 caaccacaca ggcatcgtga attttaggat gctgcaataa aatatcttcc acttcacggc
     2881 tgctaatatt ttcgccgccg cggacaataa tatctttttt gcgtccggta atttttatat
     2941 agccagcctc atccatacgg cagagatcgc cgctgtaata ccagccttct tcatccagcg
     3001 cacgggcggt taattcaggt tcatcaaaat atcccataaa cacattgggg ccacgcgagg
     3061 cttcttcacc ttcgcaacct ggcggtaagg tcttgcgtgc gtcatcgacc actttaatct
     3121 ctacacctgc ggcagcgtaa ccatcggtgt gcataaagcg cgacaaagga tcatcgagat
     3181 tcaccaccgc atgcggcgaa ctttctgtgg aaccataaac acttaataat ttaatgccga
     3241 gctgctggca ttcacgcgcc acttttttgg ggattgtggt tccgccgcaa agaaagaaac
     3301 gcagcgctga caggtccgcg ggttgtttct ctagtacatt caaaagatca tagacaaacg
     3361 gcgttgcgcc tagcatacag gtgcaacgct gctgctcaag cagcgcgaga cacgcatcag
     3421 gagtgaaaat atctaacaac acgctgcgag cgccaattaa gaatggtgcc gttacgccat
     3481 gcagaaagcc cgttgcgtgg ccgagtggcg caggcatcat aaagacatcc tgccaggtca
     3541 gattcagccg cgcgcaataa gcccgctcac tggcgagaat attgttatgc gttagcatca
     3601 cgccctttgg cagaccctcg gttccggagg taaacagcac cgcagctaat tcatcgccgt
     3661 gggtcgttat cgccgtggtc agtggggtat tgtcggcgat aatctgactt aatgagaggg
     3721 aagaggtggc gggagccagt ttgtccacgc cgacaatttg ttgtagttgt ggaagctgat
     3781 tttgcagcgg caggattaaa tctaccgggc gcgtttgttt aaacaacgtc ggtgcaaaga
     3841 acatttttgc ctgacactta ttgagcaccc acaccagttc tgcttcccgc caggaaggca
     3901 acagcggcac ggaaaccgca ccgattttca ggcaggcaag atagataacg gtaaattcac
     3961 accagccagg cagttgaaat gcgatgcgat cgcctgattc aatacccttc gctaacatcc
     4021 agtttgccag acagctcgcg gcgtgatcga gcgcgctata ggtgtacgat gcaccatgat
     4081 tatcgaccac ggcaattttg tctggcatcg cacgagcggt ctgctgccag taatcggcca
     4141 gcgaagcatc gccccatagc ccttgctgac gatacgccgc acgacgttgt tcgttgaacg
     4201 ttaatgtcac tttcatgttg gactctcgaa acagaacatc aggcccgaga tgcgggcctg
     4261 tgggatgcat gattagccgt agcgaaaatc aataccgaag gtgcctgcgg gatattgcca
     4321 ctgttcgaga atagtgttac cgcacagcac ccgacaggtg ccgcactcca gacaaccggc
     4381 agaatcaaaa tgaatgttgc cggcgtcatc ctgcttataa agtccggcag ggcaggcttt
     4441 cattaattta cggaattcat tgatatcggg atttgccgcc agaatgatat gcgggtggcc
     4501 ctcatcaaca tggaatttat tgacgcctaa tttgatgtcg acgttaaccg tagcgttctg
     4561 gctcatagcg cggttgctcc cttaatgcca tctttcagca agttgatcag cccaattttc
     4621 ttcgcatgtc ccatgatcat tttgcgtacc ggctggtttg gtttgccgtc aatggtgaac
     4681 atctcgttca tgatgtcggc gaccattcgt gggtattggc taaacaggcg cgggttttcc
     4741 atcagcgccg ggatcttgcg gaaatgctgc atatcgcgca tgatgcagct ttgttccagc
     4801 tcacgtttgt attgcgccag actgctggcg gagaaatcct cgcgttcttt ggcggcgatc
     4861 accgttgtgg cggcagcctg agccgatgca atggctaaat ccatgccacg aactgtaaaa
     4921 cccaaattca ggcagaagcc tgcggcgtca ccaacgatca tcacgccatc gttaaccatc
     4981 tgcggcacca ttgccagacc gccttctggc accatatgcg cggaatattc aagcagtttg
     5041 ccgccgctaa ttagtgggcg aatggcgggg tgttgtttaa aatcttccag catttgcggc
     5101 acgctttttt gcgcatgggc gatatcaccc aatccacaaa ccagccccaa ggatatggaa
     5161 tccttgttgg tatagagaaa tcccccgccc atcaggccgt cagaagggga accggcaaac
     5221 agccaggcgg caccttcctc gcccgtaata ttaaagcgat cgttgatctg ttctggtgtg
     5281 aggccaataa cctctttaac accaacagcg taatgatgcg gatcggaagc gggaaccatt
     5341 cccagcgagc ggccaagcat cgagttaacg ccatcagcca gaatcaccac attcgcttcg
     5401 agaatatcat ccccagcctg cacgccagtg accttgtttc cttcacgaac cagcgcatcg
     5461 acgcgaacgc ctgggataaa ctgcgcgccc gcctgctcgg cttgttccat caaccacggg
     5521 tccagacgat tacgcaatac ggtataagat gcgtgttgcg gaacatctgg ttgctcgcgg
     5581 tgaaaatcga gggtaacggc gctttcttcg gttaagaagg agattttctc gcgtgtgacc
     5641 ttgcgttcta ccggcgctga tactgcaaag cctggaatga ttgcttcaag tgtgtgggca
     5701 taaagacgcc cgccggtcat gtttttacat ccggcactgt cgccgcgttc tatcaccagg
     5761 acatccagcc cggctcgcgc catgaccagt gcggcaacgc taccagcaac gcccgcaccg
     5821 accacaatgg catcaaattt gtcttccgac atgcgttatg ctcccttccc gctgcgttta
     5881 cagcgggaga tgtggttgaa aggggaatta gcggctcaac tggctaatca gggcagggac
     5941 gactttgtag atatcgccca ccagaccgta gtcggcataa ttgaagattg gcgcattttt
     6001 atctttattg atggcgacaa tcacttttgc gccgttgccg ccaaccatat gctggatctg
     6061 cccggagatc cccagcgtca ggtagagatc ggatttcagc aacacgccgg agacaccgat
     6121 ataacgttca cgctccatcc agttctcgcc ttcggcaatt ggacgtgaac agccgacttc
     6181 agcattcagc accgccgcca gttcgtggac cattttcagg tcatcctgcg ccgccagacc
     6241 acgaccgacg ccaaccacac gttttgcttt gctcaggtcc acgctgcttg cggctttcgc
     6301 acggcgttcg cgacagagaa tttcatgacg cggagcaaca taaggtaccg tttctgtcgg
     6361 gcactgatga gaggtatcac tggtgcacgg ttcctgaaca ccgggtgcaa gggtaatgat
     6421 cgccagcggg ctgttgatct tttcctgagc gaacgctaac ccgccataca tccggtgttc
     6481 ggcgcaaatg tgaccatcga caatatccac cgccgtggca tcgttcacca gcgccgcatt
     6541 cagttgcaca cttaaccgtg ctgccagagc tttaccacgt ttcgtcgcgg ccaacagcaa
     6601 catagcgggg tgtttatctt tcagcagggc agcaatgctt tcggcgtaat tttcagtgcg
     6661 ttgcagcgcg tcgttttgct caagaacata aatacatttt ggaccataag gcataactgc
     6721 ctgcgcctgg tcggtatttt gtacaatggc atacacctgt tggccccatt gctgagcgcc
     6781 gccaaacagt tcagcataac gttcaggatt atcgctaaag acccagacgc tgtttaattg
     6841 actcatcgtt tcttctccgt aggcttcagt tcagggcttt cttcagatgt tccgccagct
     6901 cggcaatggc ctccggcgaa tcgttatcga taatgatgtg cttacgttct gtttgcggcg
     6961 gtacgcgaat gccgaccagt tcagcaagtg gcgcgctctg gctccagtca atatcacttg
     7021 cctgccactg atttaccggt tttttacccg cgccgagaat ggctttcatc gaaggaatgc
     7081 gtggcacgtt aatatcggag gtgacgcaga gcacggctgg aacagagagt tcaataactt
     7141 caacatcatc ttcaagcgtg cgttcaatca ccagtgtatt gccctgacgc tgaatagcac
     7201 tcactgcatt aatcaccgga agttgcagaa tttctccgac cagcaagcca acctgctggg
     7261 cataaaggtc gccggaacct tcaccaaaga tcagtaaatc gaagccgatc ttttcaattg
     7321 ctgccgccag cgcttttgcg gtatcgagag gcagtgcatg ttcaagttgc gcatcctgca
     7381 ccaaatacag gctgtgcggc ccgcgggata gcacgtcttt gcgcactttc gagttctgca
     7441 acaatgagcc gccaacggtc agcgcggcta tctcatcgtc atctgttgcg agctggcttg
     7501 cagcttcaat ggcattgaga tcgaactggc tgattttggc gtcggcattg tcgaaattca
     7561 gggtgtattc tggagtgaca acaatgtcct gttcttcagg caccagctta aagcaggtta
     7621 ttattttcat gatttctcct gtgagtcgca tacggaacca tctccgatgg aaggtaaaaa
     7681 tggcctgaaa atagtgtgcg atagcggtgc ggggttttcg acggagcaag ctgttttcct
     7741 tgccaacaat cttgcaaaaa aaatgcgatc gctaaaacaa aagtgcaatt tgcggcaata
     7801 tagttattcc atgaccgtac aatcttgcat tcagtgtaaa taatcttgtt tagctctcgc
     7861 taaacgcggt tgtataaccg caatacacgc cgtattttat ccggcatata ttgtttttgc
     7921 ttgctcatgg aatagggagg ttgtcatgta tcaacgctgt tttgataacg ccagtgaaac
     7981 gctgtttgtc gccggtaaaa cgccacggct ttcacgtttt gcatttagcg atgatccaaa
     8041 atgggagtcc ggacatcacg ttcatgacaa tgaaaccgag ttgatttacg tcaagaaagg
     8101 ggttgcaagg tttaccatcg attcttcgtt atatgtcgca catgcggatg acattgtggt
     8161 gatagaacgc ggcaggctac atgcggtggc ctctgacgtt aacgatccgg caacgacgtg
     8221 tacctgtgcg ctgtacggct ttcagtttca gggggctgag gaaaatcagc tactgcaacc
     8281 gcattcttgt ccggtaattg ccgcggggca gggaaaagaa gtcataaaaa ccttatttaa
     8341 tgagctaagt gtgattttgc cgcaaagtaa aaatagccaa acatcttcgt tatgggatgc
     8401 atttgcctat acgttagcca ttctttacta cgaaaatttt aaaaatgctt atcgttcgga
     8461 gcaggg
//
LOCUS       DAZFWL020000175         8408 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFWL020000175 DAZFWL020000000
VERSION     DAZFWL020000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.175"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..365
                     /locus_tag="ACHKNP_RS22060"
                     /old_locus_tag="pgaptmp_004412"
     CDS             <1..365
                     /locus_tag="ACHKNP_RS22060"
                     /old_locus_tag="pgaptmp_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706461.1"
                     /translation="IAKDFCRPGEEVSSSIASKVFHAFIDLVNKIRGKKDFMYICTLC
                     CFAEEVKGDYSHYRTFLFDIGNQYKVKLTQSGKKELSLTLEFNDTIIESQKVTGNKAK
                     HILEDIEIFYRNKPDTYY"
     gene            complement(587..1780)
                     /gene="tyrB"
                     /locus_tag="ACHKNP_RS22065"
                     /old_locus_tag="pgaptmp_004413"
     CDS             complement(587..1780)
                     /gene="tyrB"
                     /locus_tag="ACHKNP_RS22065"
                     /old_locus_tag="pgaptmp_004413"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HGU4706462.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKSRELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(2015..3112)
                     /gene="alr"
                     /locus_tag="ACHKNP_RS22070"
                     /old_locus_tag="pgaptmp_004414"
     CDS             complement(2015..3112)
                     /gene="alr"
                     /locus_tag="ACHKNP_RS22070"
                     /old_locus_tag="pgaptmp_004414"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGU4706463.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVDLSFCNI"
     gene            complement(3165..4580)
                     /gene="dnaB"
                     /locus_tag="ACHKNP_RS22075"
                     /old_locus_tag="pgaptmp_004415"
     CDS             complement(3165..4580)
                     /gene="dnaB"
                     /locus_tag="ACHKNP_RS22075"
                     /old_locus_tag="pgaptmp_004415"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU4706464.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4663..5646
                     /gene="qorA"
                     /locus_tag="ACHKNP_RS22080"
                     /old_locus_tag="pgaptmp_004416"
     CDS             4663..5646
                     /gene="qorA"
                     /locus_tag="ACHKNP_RS22080"
                     /old_locus_tag="pgaptmp_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HGU4706465.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5812..6054)
                     /gene="pspG"
                     /locus_tag="ACHKNP_RS22085"
                     /old_locus_tag="pgaptmp_004417"
     CDS             complement(5812..6054)
                     /gene="pspG"
                     /locus_tag="ACHKNP_RS22085"
                     /old_locus_tag="pgaptmp_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HGU4706466.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(6188..7225)
                     /gene="dusA"
                     /locus_tag="ACHKNP_RS22090"
                     /old_locus_tag="pgaptmp_004418"
     CDS             complement(6188..7225)
                     /gene="dusA"
                     /locus_tag="ACHKNP_RS22090"
                     /old_locus_tag="pgaptmp_004418"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HGU4706467.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNTQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7588..8295)
                     /gene="yjbM"
                     /locus_tag="ACHKNP_RS22095"
                     /old_locus_tag="pgaptmp_004419"
     CDS             complement(7588..8295)
                     /gene="yjbM"
                     /locus_tag="ACHKNP_RS22095"
                     /old_locus_tag="pgaptmp_004419"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HGU4706468.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNLGDNK
                     DAMPIEQCTFDVENKNDLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(8319..>8408)
                     /gene="yjbL"
                     /locus_tag="ACHKNP_RS22100"
                     /old_locus_tag="pgaptmp_004420"
     CDS             complement(8319..>8408)
                     /gene="yjbL"
                     /locus_tag="ACHKNP_RS22100"
                     /old_locus_tag="pgaptmp_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HGU4706469.1"
                     /translation="YRDIIIAKIDLFIQRITHNLWHARKKMCF"
BASE COUNT         2230 a         2121 c         1962 g         2095 t
ORIGIN      
        1 aaattgcaaa agatttttgt agacctggag aagaagtatc aagttctatt gcaagtaaag
       61 tttttcacgc atttattgat ttagttaaca aaatcagggg taagaaagac tttatgtata
      121 tttgcacgct ttgctgtttt gccgaggagg ttaaaggtga ttattctcat tacaggacct
      181 ttttatttga tattggtaat caatataagg ttaaacttac acaaagcgga aaaaaagagc
      241 tctctttaac tttagaattt aacgatacta taattgaatc tcagaaagtc acaggcaata
      301 aggcaaagca tattcttgag gatatagaaa tattctatcg taacaaaccc gatacttatt
      361 attaaattta ataatggtta gatgtgcaga aattattact ccacccaaaa aaacaggata
      421 aggaattcca gataattggg ataagcttaa caataaaaga actaacaacg tttattatct
      481 ctcacgtaga acgatggcat caaaaaaatc atctataaag aaagagtgaa agcgacgaca
      541 gtttaatttc actgcaggct gggtagctcc agcctgcttt cctgcattac atcaccgcag
      601 caaacgcctt tgccacacgt tgtacatttg ccgtatttaa cccggcgaca cacatgcgac
      661 cgctggcgat gagatagaca ccaaattctt cacgtagtcg gtcaacctga gcggcactta
      721 aaccggtata actgaacatg ccgcgctgat taagcagata atcgaaattg cgttctggca
      781 tctctgtgct taataccttc accagttcct gacgcattgc cagaatgcga gtacgcatct
      841 cttctacttc cgccagccag ctggctttca atgcctcgtc attcagcact gcagccacca
      901 cctgcgcacc aaaattcggc gggctggagt agttgcggcg aactgttgct ttcaattgcc
      961 ccagtacgcg gccagcggct tcggcatctt cacacagaac agaaagtccg ccgacgcgct
     1021 cgccgtaaag ggagaaaatt ttcgagaacg aattgctcac cagagcgggt aatccagcgc
     1081 tggcaatggc gcgaatagcg taggcatcct cttccatacc ggcaccaaat ccttgatagg
     1141 caatatcgag gaatggaata agctcgcggg atttgagaat ttcaatcacc gcatcccact
     1201 gatcattagt gagatcggca cccgttgggt tgtggcaaca tggatgcagc aacacaatac
     1261 tgcgggcagg taatgttttc agcgtcgcca acaggtcatt aaagcgcacg ccgttagtcg
     1321 cttcgtcata ccaggggtaa gtactcactt cgaatccagc cccggcgaat attgctacgt
     1381 ggttttccca ggtaggatcg ctgacccaga cgcctgattc cgggaagtag cgtttcagga
     1441 aatccgcgcc cactttcaat gcccctgagc cgccaagggt ttgaatggtt gctacgcgct
     1501 gttgttgcag tactggatgg tccgcaccaa acagcagcgg cgcaatggca tggcgatagc
     1561 tgttaagccc ttccatcggt aaataaagcg aagcgccatg aggctgcgca ttcaggcgcg
     1621 cttccgcctc cgccacggct tgcagttgtg gaataattcc gtcttcgttg tagtacagac
     1681 cgatacttaa attcactttg tcgctgcgag ggtcttcttt aaaacgctcc ataagcgtaa
     1741 gaatcgggtc gccagcgtag gcgtcaactt tttgaaacac gcgatggttc tccaggttta
     1801 cgggcaggtg gttaaaacac aataaaccgg aagaaggcga agatcgagtg gatgttcagg
     1861 agcgaacggc aattagcaac agagtgagac tcatgacaaa cgtacatccg ccagaggcac
     1921 gacctttata agagcatgaa agaacatcaa cttattgaat ttttaggatt ttattggccg
     1981 gataaggcat tcacgccgca tccggcacag acaatcaaat attacagaac gataaatcca
     2041 cgtatttcat cgcgaccctt gaagtcaggc gcgtaataag ttcgtaagcg cttactttcg
     2101 tcatttcagc gatacgttct acgggcaaac cttcgcccca taaaatgacc ggatccccgg
     2161 ctttgtcctg cgcttgtgga cctaagtcta cgcagatcat atccatcgcc acgcgcccga
     2221 caatcggtac ttcgcgaccg ttcaccagca ctggcgtacc ggacggcgcg gcgcgcggat
     2281 aaccatcgcc atagcccatc gcgactacgc caagacgggt atcacgttcg cttacccagg
     2341 ttccaccata accaacaggt tctccggctt tatgctcacg cacggcaatc aggctggagg
     2401 ttagtgacat cactggctga cagccaaaat cggcaccggt ggagcgatct tccagcggcg
     2461 agacgccata aagaatgatg cccgggcgca cccagtcaaa atgcgactgt ggccacagca
     2521 gaatgccacc cgatgcggca atggaacgtt gaccaggttt gccttcgcaa aaggtattaa
     2581 agatagcgag ttgtttctcg gttgcgccac attttggttc atccgcgcgc gcaaaatggc
     2641 tgacgatatt caccggctga cgaacgtttt tgcactgggt caggcgatga taaaacgcct
     2701 cagcctgttc cggccttacg cccagacggt gcataccggt atcgagtttc atccagacgg
     2761 taaccggctc gtccaggcta gcctcttcca gcgcagccag ctgttcttcg ttatgcacgg
     2821 cggtatgaaa atgttgcgca gaaatcgtcg gcagatctct ggcatcaaaa aagccttcga
     2881 gtaacagtac aggtttggtg attccccccg cacgcagtcg cagagcttct tcgagacggg
     2941 ctacgccaaa ggcgtcagca tcggggagcg ttcgcgcggt ctcaagaaga ccgtgaccat
     3001 aagcgttcgc tttcaccacc gcaaccattt tactggcagg ggccagttca cgaagacgtt
     3061 gcaggttgtg tcgcagagcg cggcggttaa tcacaacagt tgccgcttgc atttgtgttc
     3121 cttgataagt gtttgcttta attacctaat tcataaaata attattattc gtcgtcgtac
     3181 tgcggccccg catagttgtc gaagcgcgac cattgaccgt taaaggtcag gcgtaccgtc
     3241 ccgattgggc cgttacgttg tttaccgata ataatttccg cgatgccttt taaatcactg
     3301 ttttcgtgat acacctcatc acgatagata aacatgatca agtccgcatc ctgctcgata
     3361 gagccagatt cacgcaggtc ggagttgacc gggcgtttgt cggcacgttg ttccagagaa
     3421 cggttcaact gggacagcgc caccaccggc acgttcagtt ctttcgccag tgctttcagc
     3481 gagcgggaga tttctgcaat ttccagcgta cggttatcgg aaagcgccgg tacgcgcatc
     3541 agttgcaggt agtcgatcat gataagcccg atgccgccgt gttcacgggc aatacggcgt
     3601 gcgcgggaac gcacttccgt tggcgtcagg ccggaggagt catcgatata gatattgcgt
     3661 ttttcgagca aaatacccat ggtgccggaa atgcgcgccc agtcttcatc atcgagctgc
     3721 ccggtacgga ttttagtctg gtcaacgcgc gacagcgacg ccagagaacg catcataatc
     3781 tgttctgaag gcatctccag cgagaagata agtactggtt tatcctgcaa catcgccgcg
     3841 ttttcgacga ggttcatcgc aaatgttgtt ttacccatcg acggacgcgc ggcgacgatg
     3901 atcaaatccg acggctgcaa gccagcggtt tttttgttga gatcgtcata accggtgttt
     3961 actccggtaa cgccatcgtg tggctgctga aacaactgct caatacgcgc cacggttgcg
     4021 tcgagcacat cggcgatgtt cttcggccct tcgtctttgt ttgcacgact ttcggcaatt
     4081 ttaaagacgc gggattcagc aaggtccagc agatcttcgc tggtacgccc ctgcggatca
     4141 aaaccggctt cagcaatctc attcgcaacc gagatcatct cacggacaac ggcacgttca
     4201 cgcacgatgt cagcataagc actgatgttc gccgcacttg gcgtattttt tgacagctct
     4261 gccagataag caaaaccacc gacgctatcg agttgcccct ggcgttccag cgattccgca
     4321 agggtaatca gatcgatagg actaccgctt tcctgcaaac gcgccatttc agtaaagata
     4381 tggcggtgtg ggcgggtgta aaaatcgtct gccaccacac gctcggctac atcgtcccag
     4441 cgttcgttat ccagcattaa accgcccaac accgactgct ccgcttcgat cgagtgcgga
     4501 ggcactttca gcccggcaac ttgtggatcg cgttcgcggg gttcagcctg ctgtttgttg
     4561 aagggtttat ttcctgccat agtgaatgga gttaccgaga taaagaatgg gtcgaaactt
     4621 taccatatga agcagcccct gacgatacgt tctggaggac acatggcaac acgaattgaa
     4681 tttcacaagc acggtggccc ggaagtactt caagccgtag agttcactcc tgccgatccg
     4741 gcagagaatg aaatccaggt cgaaaataaa gccatcggca tcaattttat cgacacgtat
     4801 atccgcagcg gcctttaccc gccgccatca ctacccagcg gattaggtac cgaagcggca
     4861 ggcatcgtga gtaaagtcgg cagtggtgta aagcatatta aggcaggcga tcgtgttgtc
     4921 tatgcgcagt cggcgttagg cgcttacagc tctgtgcata acattaatgc ggataaagcg
     4981 gcgattctgc ctgcggcaat ttcttttgag caagctgcgg catccttcct gaaaggctta
     5041 acggtttatt atctgctgcg taaaacctat gaaattaaac ccgatgaaca gttcctgttc
     5101 cacgcagcgg ctggcggcgt tggcttgatt gcctgccagt gggcaaaagc cctgggcgcg
     5161 aaacttatcg gcaccgtagg aaccgcgcaa aaagcgcaga gcgcgctaaa agcgggcgcg
     5221 tggcaggtaa ttaactatcg tgaagagaat ctggtcgagc ggttaaaaga gatcaccggc
     5281 ggtaagaaag tgcgcgtggt gtacgattcc gtgggcagag acacctggga acggtcgctg
     5341 gattgcctgc aacgccgcgg cttaatggtc agttttggca actcatcagg tgcggttacc
     5401 ggtgtgaact taggcattct caatcaaaaa ggctcgttgt atgtgacacg cccttccctg
     5461 caaggctata tcaccacgcg ggaggaatta accgaggcca gtaatgaact gttctctttg
     5521 attgccagcg gtgtgattaa ggtcgatgtc gccgagcagc agaaatatcc gctgaaggat
     5581 gcgcagcgtg cgcatgagat tctggaaagc cgggcgacgc aaggttccag cctgctgatt
     5641 ccataaaaga aatagggctt ccacctggga agccctttct ttttatagtt cggctgtatg
     5701 tagggtacag cacgatgaat ctgttagagg cgcaatagtg acagatttga ttatcaattc
     5761 ctattttgtt ctaaggataa aaccttaggt tgtgatcatc cgcacaatcc cttagtaacg
     5821 ccagcggtca taacgctgat atttaggcac ttttggtgct ttaatcgcct taataaccca
     5881 caccaccgca atcgccagta gtaaccacgg cagcaactta atcatcaatg ccagcatacc
     5941 gccgaggaac ataatggccg tcgccacaac cagcgcggcg ataatgccca gcaacgaaac
     6001 gccggtgacc atcagcatga caaaaaagcc aatcacaaaa agtagttcca gcatgatgct
     6061 ctcccaaata tgaaatctct tgctggcatt acaagaatca tgccaaaaat aatctattga
     6121 tttaacagca aaacgccccg cgacggtgcg cagggcgtgg tgaatttgac tactttttgg
     6181 tgaaaagtta acgcttatcc gccaccagtt tgagcgcgtg ttccagcaca ttaatgtctg
     6241 cacccgcttt atgggcattt tcacttaaat aacgccgcca ctgccgcgcg ccaggaatac
     6301 cctggaacaa gcccaacata tgccgggtaa tatggccgag atacgtcccc tggctgagtt
     6361 cacgctcaat gtacggatac atggcgcgca ctaccgccac cggatcggca tcggtatccg
     6421 aggaaccaaa gatctcccgg tctaccgccg ccagaatacc cggattctga tacgcctcgc
     6481 gcccgaccat cacgccatcc atatgttgca ggtgtgcttt ggcctcttcc agcgacttga
     6541 taccaccgtt aatcgacatt gtcagatgcg gaaagtcacg cttcagttga tacacacgcg
     6601 gataatcgag cggcgggatt tcacggtttt ctttcggact taaccccgaa agccaggctt
     6661 tacgtgcgtg gatgataaac atctcacact cgcctttgcc ggaaacggtg ttgatgaaat
     6721 cgcagagaaa ttcatagctg tcctggtcat cgatgccaat acgcgttttc accgtcactg
     6781 gaatcgacac cacatcgcgc atcgctttca cgcagtcggc aaccagctgc gtattaccca
     6841 tcagacacgc accaaacatg ccgttctgca cccggtcaga cgggcagccg acattcaggt
     6901 tgatctcatc atatccacgc gcttctgcca gcttcgcaca ctgtgccagc gccgccggat
     6961 cgctaccgcc gagttgcaac gctaccggat gttcttcttc actgtacgcc aggtaatcac
     7021 ctttaccgtg aataatcgcc cctgtggtca ccatttcggt atacagcaac gtattgcggg
     7081 aaagcagacg caagaaatag cggcaatgtc tgtccgtcca gtcgagcatt ggtgcaacgc
     7141 taaaccgacc actccagtga acgtcagttt tttcaggcat tgcgctggtt tggttgattt
     7201 tttgcatttc agaattaccg tgcattttca aatgtagaga ttattttatc gatatatcat
     7261 ggggttatgt tattcagcat cactattcag gaggctcaat agcggggtac tataccataa
     7321 caacaggaag cgcctgtctc attgcaaaag aaaattgaga tcctctcaag gcatgaagct
     7381 ctcacgaagt gatggaaata atcttattag ccgttaactt tgttaaggcc aatgataaac
     7441 aatccaggtt cgacgataaa taaaaaatca cacattaaac tctggtgata tatctccctg
     7501 ctaatagcat tgatagagaa aaaagaaccc aataaatatt gggtcctttt atataatgcc
     7561 ttccatacta tcgaagaact tcacatatta tttctccgat ttaaccccga acaaatcata
     7621 aattaattta gaagtgtctg taagtatttt aatctcttcc tttgaggttg ggtcaaacga
     7681 cttcgcgaag ctaattaatt gtggtgcagc atctcgcatt ttgcttaaaa tatcaggatc
     7741 gagcgttcct tcattcacca gatgtgacat cttatccaga tagccatcaa aattcatttc
     7801 tcccccatca gccagacgct tcatctctcc caggtacttc ttcatatcac gttgagataa
     7861 tttttcaaac tgagctttca ggtagctctc attattttca ttaatataca ctgtttctga
     7921 taaatctgcg taggcactag aatatgaaag tgaaattaac agcgaagcaa ataattttaa
     7981 gctgttgtca tcatgttcct ggcaggcatt aacaaacgtt aaaaaaccag aacctattgc
     8041 ttgaaaatgt atattcgcta aaggttcatt atccttagat tcctcataaa atagctgaac
     8101 cgtggaggga aggtcattct tattttctac atcaaaagta cattgttcaa tgggcattgc
     8161 atctttatta tccccaagat tacatatact attaatcgcg aaatgaaaaa tagcccggtc
     8221 aacaactgat gcaataaagt cacgatcgtt taaatcctca tcagtgcaat cgtcaatata
     8281 cttattgacc cacattttga gatcatttat ttgctcaatt aaaaacacat ttttttacgt
     8341 gcatgccata aattatgcgt gattcgttga ataaacaaat ctatttttgc aataattata
     8401 tcccgata
//
LOCUS       DAZFWL020000176         8265 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFWL020000176 DAZFWL020000000
VERSION     DAZFWL020000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.176"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1203
                     /locus_tag="ACHKNP_RS22105"
                     /old_locus_tag="pgaptmp_004421"
     CDS             <1..1203
                     /locus_tag="ACHKNP_RS22105"
                     /old_locus_tag="pgaptmp_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HGU4706470.1"
                     /translation="VLLKALNPTINTQVNDVMSLPFIKMEAAVQEKIETNEQLLINIA
                     MHDWNSQEISFDFMVNSLVSESRASSEVASSYAIARKKWQDAFNKTKELEEENNHLVI
                     TSSNFNGINEELIDSEITLVSNPYYKYGDSTTNNETRLLSDSIADVLSYSIGCMMGRY
                     SLDREGLVYAHEGNKGFAELAAEGAYKTFPADNDGILPLMDDEWFEDDVTSRVKEFVR
                     TVWGEEHLQENLEFIAESLCLYAIKPKKGESALETIRRYLSTQFWKDHMKMYKKRPIY
                     WLFSSGKEKAFECLVYLHRYNDATLSRMRTEYVVPLLARYQANIDRLNDQLDEASGGE
                     ATRLKRERDSLIKKFSELRSYDDRLRHYADMRISIDLDDGVKVNYGKFGDLLADVKAI
                     TGNAPEAI"
     gene            1380..3977
                     /gene="pglZ"
                     /locus_tag="ACHKNP_RS22110"
                     /old_locus_tag="pgaptmp_004422"
     CDS             1380..3977
                     /gene="pglZ"
                     /locus_tag="ACHKNP_RS22110"
                     /old_locus_tag="pgaptmp_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HGU4706471.1"
                     /translation="MQNQDFIAGLKAKFAEHRIVFWHDPDKRFIEELEQLKLENVTLI
                     NMTHASQLAVKKRIEMDEPEQQFLLWFPHDAPPHEQDWLLDIRLYSSEFHADFAAITL
                     NTLGIPQLGLREHIQRRKAFFSTKRTQALKNLVTEQEDETSLDKKMIAVIAGAKTAKT
                     EDILFNLITQYVNQQTEDDSELENTQAMLKRHGLDPVLWEMLNHEMGYQAEEPSLENL
                     LLKLFCTDLSAQADRQQRAWLEKNVLLTPSGRASALAFMVTWRADRRYKEAYDYCAQQ
                     MQAALRPEDHYRLSSPYDLHECETTLSIEQTIIHALVTQLLEESTTLDREAFKKLLSE
                     RQSKYWCQTQPEYYAIYDALRQAERLLNLRNRHIDGFHYQDSATFWKAYCEELFRFDQ
                     AYRLFNEYALLVHSKGAMILKSLDDYIEALYSNWYLAELSRNWNEVLEAENRMQAWQI
                     PGVPRQQNFFNEMVKPQFQNPQIKRVFVIISDALRYEVAEELGNQINTEKRFTAELRS
                     QLGVLPSYTQLGMAALLPHEQLCYQPGNGDIVYADGLSTSGIPNRDTILKNYKGMAIK
                     SKDLLELKNQEGRDLIRDYEVVYIWHNTIDATGDTASTEDKTFEACRTAVTELKDLVT
                     KVINRLHGTRIFVTADHGFLFQQQALSVQDKTTLQIKPENTIKNHKRFIIGHQLPADD
                     FCWKGKVADTAGVSDNSEFLIPKGIQRFHFSGGAHFVHGGTMLQEVCVPVLQIKALQK
                     TAAEKQPQRRPVDIVAYHPMIKLVNNIDKVSLLQTHPVGELYEPRILNIYIVDNANNV
                     VSGKERISFDSDNSTMEKRVREVTLKLIGASFNRRNEYWLILEDAQTETGYQKYPVII
                     DLAFQDDFF"
     gene            3988..6072
                     /gene="brxL"
                     /locus_tag="ACHKNP_RS22115"
                     /old_locus_tag="pgaptmp_004423"
     CDS             3988..6072
                     /gene="brxL"
                     /locus_tag="ACHKNP_RS22115"
                     /old_locus_tag="pgaptmp_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HGU4706472.1"
                     /translation="MQTHHDLPVSGVSAGEIASEGYDLDALLNQHFAGRVVRKDLTKQ
                     LKEGANVPVYVLEYLLGMYCASDDDDVVEQGLQNVKRILADNYVRPDEAEKVKSLIRE
                     RGSYKIIDKVSVKLNQKKDVYEAQLSNLGIKDALVPSQMVKDNEKLLTGGIWCMITVN
                     YFFEEGQKTSPFSLMTLKPIQMPNMDMEEVFDARKHFNRDQWIDVLLRSVGMEPANIE
                     QRTKWHLITRMIPFVENNYNVCELGPRGTGKSHVYKECSPNSLLVSGGQTTVANLFYN
                     MASRQIGLVGMWDVVAFDEVAGITFKDKDGVQIMKDYMASGSFSRGRDSIEGKASMVF
                     VGNINQSVETLVKTSHLLAPFPTAMIDTAFFDRFHAYIPGWEIPKMRPEFFTNRYGLI
                     TDYLAEYMREMRKRSFSDAIDKFFKLGNNLNQRDVIAVRRTVSGLLKLMHPDGAYSKE
                     DVRVCLTYAMEVRRRVKEQLKKLGGLEFFDVNFSYIDNETLEEFFVSVPEQGGSELIP
                     AGMPKPGVVHLVTQAESGMTGLYRFETQMTAGNGKHSVSGLGSNTSAKEAIRVGFDYF
                     KGNLNRVSAAAKFSDHEYHLHVVELHNTGPSTATSLAALIALCSILLAKPVQEQMVVL
                     GSMTLGGVINPVQDLAASLQLAFDSGAKRVLLPMSSAMDIPTVPAELFTKFQVSFYSD
                     PVDAVYKALGVN"
     gene            complement(6337..6762)
                     /locus_tag="ACHKNP_RS22120"
                     /old_locus_tag="pgaptmp_004424"
     CDS             complement(6337..6762)
                     /locus_tag="ACHKNP_RS22120"
                     /old_locus_tag="pgaptmp_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4706473.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCGQHF
                     PGKKFCEACGTGIYSRAVCTKYVYSYQPDLFQDCGSAST"
     gene            complement(6759..7142)
                     /locus_tag="ACHKNP_RS22125"
                     /old_locus_tag="pgaptmp_004425"
     CDS             complement(6759..7142)
                     /locus_tag="ACHKNP_RS22125"
                     /old_locus_tag="pgaptmp_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4706474.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            complement(7514..8113)
                     /locus_tag="ACHKNP_RS22130"
                     /old_locus_tag="pgaptmp_004426"
     CDS             complement(7514..8113)
                     /locus_tag="ACHKNP_RS22130"
                     /old_locus_tag="pgaptmp_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4706475.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
BASE COUNT         2173 a         2043 c         2087 g         1962 t
ORIGIN      
        1 gttctactta aagcattgaa ccctactatt aatacccaag ttaatgatgt catgtcatta
       61 ccattcatca aaatggaagc agctgttcaa gaaaaaatcg aaactaatga acagctgctg
      121 ataaatatag cgatgcacga ttggaattcg caagaaattt cttttgattt tatggttaat
      181 tctttagtca gcgaatcgag agcatcatct gaagtagcat caagctatgc tatagcaaga
      241 aaaaagtggc aagatgcttt taataaaacg aaagagttgg aagaagaaaa taaccattta
      301 gtcattacaa gtagcaactt taatgggata aatgaagaat taattgattc agaaataact
      361 ttagtcagca acccatacta caagtacggt gattcaacga ctaataatga aactcgtttg
      421 ttatcggatt caatagcaga tgtattaagt tattccatcg gctgcatgat gggtcgctac
      481 tccctcgatc gcgaaggcct cgtctacgcc catgaaggca acaaaggctt tgccgaactt
      541 gccgctgaag gtgcgtacaa aaccttcccg gcagacaatg acggcatcct gccgctgatg
      601 gatgacgagt ggtttgagga cgacgtcacc tctcgcgtca aagagtttgt ccgcaccgtc
      661 tggggcgaag agcacctgca ggaaaacctc gaatttatcg ccgaaagtct ctgtttatac
      721 gcgatcaagc cgaaaaaagg cgaatctgcg ctggagacca ttcgtcgcta tctttccaca
      781 cagttctgga aagatcatat gaagatgtat aaaaagcgcc caatctactg gctattcagc
      841 tccggtaaag agaaagcctt cgagtgcctg gtgtatctgc atcgctataa cgacgccacg
      901 ctgtcgagaa tgcgtaccga atatgtggtg ccgctgctgg cacgttatca ggccaatatc
      961 gatcgcctga acgatcaact tgatgaagct tctggcggtg aagccacacg cctgaaacgc
     1021 gaacgcgaca gcctgatcaa aaaatttagc gaactgcgta gctatgacga ccgcctgcgt
     1081 cactatgctg atatgagaat cagtattgat ctcgacgatg gcgttaaggt taactacggc
     1141 aagtttggcg atctgctggc agatgtcaaa gccatcaccg gcaatgcccc agaggcgatc
     1201 taaaccagac ggcgcgttcc cctactgccg ggttctgccc ggtggcaaat accaccggga
     1261 aacgcgccgc tgctggcatt tctccacctc acttcatgat aaaatgcgcc accgtgtcaa
     1321 aatctccttt tcgcgttttg gcgctttctt attcatcgta acaacatggg attgtgaact
     1381 tgcaaaatca ggactttatt gctggcctta aagctaaatt tgccgaacat cgcatcgttt
     1441 tctggcacga tcccgataaa cgttttattg aggaactgga acagctcaag cttgaaaacg
     1501 tcacgctaat caacatgacc cacgcgtcac agctggcggt gaaaaaacgc atcgagatgg
     1561 atgagccaga acagcagttc ctgctgtggt tcccccatga tgcgccgcct catgaacaag
     1621 actggctgct ggatatccgc ctttacagca gcgaattcca tgccgatttt gccgccatca
     1681 ccctgaacac gctgggcatt ccccagcttg gcctgcgcga gcatattcag cgacgcaagg
     1741 ccttcttcag cactaaacgt acgcaggcgc tgaaaaacct ggtgacggaa caggaagatg
     1801 aaacctcgct ggataagaaa atgattgcgg tgatcgctgg cgcaaagacc gcgaaaaccg
     1861 aagacatttt gtttaacctg attacccagt acgttaacca acaaacagaa gacgacagcg
     1921 aactggaaaa cacgcaggcg atgttgaaac gccacggtct ggacccggta ttgtgggaaa
     1981 tgctcaacca cgaaatgggc taccaggcag aggagccatc gctggaaaac ctgctcctga
     2041 aactgttttg taccgatctc tctgcccagg ccgacagaca gcagcgcgcc tggctggaaa
     2101 aaaatgtcct gctgacgcca tccggcagag catccgccct ggcatttatg gtgacctggc
     2161 gtgccgatcg tcgctataaa gaggcttatg actactgcgc tcagcaaatg caggcggccc
     2221 tgcgcccgga agatcattac cgactcagtt cgccgtatga tttgcacgaa tgcgaaacca
     2281 ccctcagcat cgaacaaacc attattcatg cgctggtaac gcaactgctg gaagagagca
     2341 ccacgctcga tcgggaagcc tttaaaaaac tgctctctga acgccagagc aaatactggt
     2401 gccagacaca accagagtat tacgccatct atgacgcgct gcgccaggct gagcgtttgc
     2461 tgaatctgcg caatcgccac atcgacggtt tccactatca ggacagcgcc accttctgga
     2521 aagcctactg cgaagaactg ttccgcttcg accaggctta tcgcctgttt aatgaatatg
     2581 ccctgctggt tcacagcaaa ggggcgatga tcctcaagag cctggatgat tatatcgagg
     2641 cactctacag caactggtat ctggcagaat taagccgtaa ctggaatgaa gtgctggaag
     2701 cggaaaatcg tatgcaggcg tggcaaattc ctggcgtgcc gcgtcagcag aacttcttca
     2761 atgagatggt gaagccgcag ttccaaaatc cgcaaatcaa acgcgtgttc gtgataattt
     2821 ccgatgccct gcgttatgaa gtggcggagg agctggggaa tcaaatcaat accgagaaac
     2881 gctttaccgc agaactgcga tcgcagctcg gcgtgctccc tagctacacc caactgggaa
     2941 tggcggcgtt gctgccccat gaacaacttt gctatcaacc cggtaacggc gacatcgttt
     3001 atgctgatgg gctgtcgacc tcgggtattc ctaaccgcga taccattctg aagaactata
     3061 agggaatggc gataaaatcg aaggaccttc tggagttaaa aaaccaggaa gggcgagacc
     3121 ttattcgcga ttacgaagta gtgtatatct ggcataatac gattgatgcc actggcgaca
     3181 cggcatccac ggaagataaa accttcgaag cgtgccgcac ggcggtgact gaactgaaag
     3241 atttagtcac caaggtaatc aaccgtctcc acggcacacg catttttgtt acggcggatc
     3301 acggtttcct gttccagcaa caggcgcttt cggttcagga taaaaccact ctgcaaatta
     3361 agccggaaaa caccatcaag aaccacaaac gctttattat cggccaccag cttcccgccg
     3421 atgatttttg ctggaaaggg aaagtggcgg ataccgcagg cgtgagcgac aacagcgagt
     3481 tccttattcc gaaagggatc cagcgcttcc atttctctgg cggcgcgcac ttcgttcatg
     3541 gcggcaccat gttgcaggag gtttgcgttc cggtattgca gataaaagcc ctgcaaaaaa
     3601 ccgccgcaga aaaacagccg cagcgccgcc cggtggatat tgtcgcttac catccgatga
     3661 ttaagctggt gaacaatatc gataaagtga gcctgttgca gacgcatccg gtgggcgaac
     3721 tttatgaacc gcgtatcctg aacatttaca ttgtcgacaa cgccaacaat gtggtctcgg
     3781 gcaaagagcg catcagcttt gacagcgata acagcaccat ggaaaaacgc gtacgcgaag
     3841 ttacgctgaa gctgattggc gctagcttca accgtcgcaa tgagtactgg ttgatactgg
     3901 aagacgcaca aacggaaacg gggtatcaga agtacccggt cattatcgat ctggcgttcc
     3961 aggatgattt cttctaagtg aggcgatatg caaacccatc atgatttacc tgtttcaggc
     4021 gtatccgcag gggaaattgc ctccgagggt tacgatctgg acgccctgct gaaccagcat
     4081 tttgctggtc gcgtggtgcg taaagatctc accaagcaac tcaaggaagg ggcaaacgtc
     4141 ccggtgtatg tgcttgagta tctgctcggc atgtactgcg cctctgacga tgacgacgtg
     4201 gtcgagcaag ggttgcaaaa cgttaagcgt attctggctg ataactatgt gcgcccggat
     4261 gaagcggaga aagtgaagtc gctgatccgc gagcgtggtt cgtacaaaat catcgataaa
     4321 gtgtcggtga aactgaacca gaaaaaagac gtttacgaag cccagctttc taacctcggc
     4381 atcaaagacg cgctggtgcc ctcgcagatg gttaaagaca acgagaagct actgacgggc
     4441 ggtatctggt gcatgattac cgtcaactat ttctttgaag aagggcagaa gacctcaccc
     4501 ttctcattga tgacgctcaa gcctatccag atgccgaata tggatatgga agaagtgttc
     4561 gatgcgcgta aacactttaa ccgtgaccag tggatcgatg tgctgctgcg ctcggtgggt
     4621 atggagcccg ccaatattga gcaacgcacc aaatggcacc ttatcacccg tatgatcccg
     4681 ttcgtggaga acaactataa cgtttgcgag ctggggccgc gtggcaccgg taaaagccat
     4741 gtgtataaag agtgttctcc taactccctg ttagtttccg gcgggcaaac gaccgttgcc
     4801 aacttgttct acaacatggc cagtcgccag atcggcctgg ttggcatgtg ggatgtggta
     4861 gcgttcgacg aagtcgcggg gatcactttc aaagataaag acggcgtgca aatcatgaaa
     4921 gattacatgg cgtcaggatc tttctctcgc ggcagagatt cgattgaagg taaagcgtcg
     4981 atggttttcg tcggcaacat caatcaaagc gtagagactc tcgttaaaac cagccatttg
     5041 ctggcaccat ttccgactgc gatgattgat acagcatttt tcgaccgctt tcatgcctat
     5101 attcccggtt gggaaatccc caaaatgcgc ccggaattct ttaccaaccg ttacgggctg
     5161 attacggatt atctcgctga atatatgcgc gaaatgcgca aacgcagttt ctctgatgcg
     5221 attgataaat tctttaagct gggtaacaac ctcaaccagc gtgacgttat tgccgttcga
     5281 cgtaccgtgt cggggttgtt aaaactcatg catcccgatg gcgcgtacag caaagaagat
     5341 gtgcgagtct gcctgaccta tgcgatggaa gttcgccgcc gcgtgaaaga gcaacttaaa
     5401 aaactgggcg gtctggagtt cttcgatgtg aactttagct acatcgacaa cgaaacgctg
     5461 gaagagtttt ttgtgagcgt accggaacag ggcggcagcg aacttattcc tgccggaatg
     5521 ccaaagccgg gtgttgtgca tctggtcact caggcagaaa gcggcatgac cgggctgtat
     5581 cgttttgaaa cacagatgac tgccggtaat ggtaagcata gtgtatcggg tctgggttca
     5641 aatacctccg cgaaagaagc tatccgcgtc ggtttcgatt acttcaaagg caatttgaat
     5701 cgggtaagcg cggccgcgaa attctccgat catgaatatc accttcatgt cgttgaactg
     5761 cataatactg gcccaagcac cgcaaccagt cttgctgcgc ttatcgcttt atgttcgata
     5821 ttgctggcaa aaccggtgca ggaacagatg gtggtgttgg gcagtatgac gcttggtggg
     5881 gtaattaacc cggtgcagga tcttgccgcc agtttgcagc tcgccttcga cagcggtgca
     5941 aaacgggttc tgttgccgat gtcctcggct atggatattc caacggttcc ggcagagtta
     6001 tttaccaagt ttcaggtgag tttttactca gacccggttg atgctgttta taaggcgctg
     6061 ggtgtgaatt aacgtagtaa ctattttaat gaactggaga ggacaatggc tcaagaacgt
     6121 tctgtaatta aattcatcag accttgcctc tccattcatg aatataaaaa tacttacatc
     6181 tgagtattgt tggctgtgtt tgaataaaaa gagagctgtt caaataagcc tgtataactg
     6241 ttctttacaa aaaatggagg agctatcagc taattcaaca ctggaagatg ttcgtcgagt
     6301 tggattgaac gccctgcgca gaccaggcat ttcggattaa gtgctcgcac ttccacagtc
     6361 ctggaacaag tctggctggt agctgtaaac atactttgta cataccgctc tggaatagat
     6421 ccccgtgcca caggcttcac agaacttttt cccgggaaaa tgctgcccgc acatcacaca
     6481 atgccactcc agcacgaccg gtaatggcga taaaaacatc gccatatcct caatgtaagg
     6541 gtgggacttc tccggattca gcaccacaca gtccgccttc tgttgctcac tcagggcatg
     6601 taaatcatgc tcaaaccacg ccccctgagc atctgtctgc agaatcaacc ggccatggca
     6661 ggaaaggcag aaacactgcc ggatatttct gctgagcgca gagtcggcac tgataatgtg
     6721 ctcacccggc gtgatcccca gccccgtttt tataccgttc attctgctcc cccctcctca
     6781 ctgaagcgcc cggtctggca gtgtgtgcag tactgctccc cataataatc gtggtgacat
     6841 tgcctgcagt gccagctggc tttacgtact acgggtaagg catccgggac gaattgctgc
     6901 agacgtttaa tcaaccgtat ttctctgcgt tccggcctga cataagggca ctcatgaccg
     6961 tgctccgtca gcccttcgtc agcgtgttca aaccagggcc gttcagtgtc gtattgcggg
     7021 tggtatctga gtgcactgcc gcaaaggtgg caggtgtagc tatcgtaagg tgcagtctgt
     7081 gcggtacggg caccggtcag acgtccgttg ccatcaagtg cgagaaaaga ttttgcgtac
     7141 atagtatatg ttcctctgcg ccagacgaca cgcaggcaac agccgtcccg gtacgggcag
     7201 cgtggtcagg gtgtgaatgg cggttcagtt aaaatctgga ctggagtttg ggtaaagcag
     7261 gctggttgct gttacagcac tgtggttgtc acacaatggc ccactgacgt aaaaaacagt
     7321 cagtaagcag agatggagat tgggggatgt gctgtatcgg ttgccggaag gaatggtaaa
     7381 gaatgtgtca tggttaaatc tccgtatggt tcatacggaa tgaccacgcc gtaaaaaatg
     7441 cgcagtgccg ctgaagtggc cgccggaacc gacagcaaag attagcgcca gcccgtactt
     7501 gtcgttcagg ggtttacacc agagcacata tccccggcag ggggctgtcc agtcgtggtc
     7561 gaccccggtt acttttttca ggaagcccgc tgcatttcag gatgatgcca cattctttct
     7621 gttcccgttt ggccaggtag ctgatggcat agcgtaaatc attcaccgct ttgtgatctg
     7681 aatagtgaat gacgtggtta atgtttaccg gataattttt attaaaccgg caccagtggt
     7741 agtaaccatt accctcggta acccgcctcc agatatctcc catcaatctg gataccagat
     7801 acgaactcct gtggctctgg ccgttcagat agcccacaaa atgaatgtga agccctttac
     7861 gttctgtata ttccatcacc caggcatacc cgaccagttt ctctcccacc tcgttaatca
     7921 gttgcagcat ctccgcgcac atggcgtacc tgcaggcaca tctgtagctg agggtgtttt
     7981 ttcggtaagc aaaatccatt ctgaacggta acagtttcga atagaccccg aataatgaat
     8041 ccagatgctg gttaaagtgc tccaggagct ggggagccat ttcgtaagcg ggattggtgt
     8101 tataagtttg catgtttctt cctcgtgttc tgtaacacag gaagaagcgt tgccgtaatt
     8161 tttgaagaag atgttctcag gtagtagggt gaacgtgttt cctccggtaa cgacttccac
     8221 gggtatcaga accaggcaaa gaacagcata cgtaacagcc ataca
//
LOCUS       DAZFWL020000177         8263 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFWL020000177 DAZFWL020000000
VERSION     DAZFWL020000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.177"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..938)
                     /locus_tag="ACHKNP_RS22135"
                     /old_locus_tag="pgaptmp_004427"
     CDS             complement(<1..938)
                     /locus_tag="ACHKNP_RS22135"
                     /old_locus_tag="pgaptmp_004427"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HGU4706476.1"
                     /translation="MFSALDIKTLMQGTLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSNAKAKIVVDYLNVNNLNKVLRYFKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMSDERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGHDCIIGNKCLIVSQCGFSGHVVLGDHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAKNTREYNKNLAVLNKLTKQ"
     gene            complement(957..1994)
                     /locus_tag="ACHKNP_RS22140"
                     /old_locus_tag="pgaptmp_004428"
     CDS             complement(957..1994)
                     /locus_tag="ACHKNP_RS22140"
                     /old_locus_tag="pgaptmp_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU4706477.1"
                     /translation="MVLIKKNVLSASIATVLLGSTGAFAANDRGFTSDTDWSEEQAGF
                     NGHVGTTFEYETKSTDNYLGKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQE
                     EKGYHENETSLQNYISLNKTFTIGNGFDFSLVYDLMYTDGNIDSPYVTGLHTVTVDNS
                     FRPTLTYFNSDWNAGFYSNVEYLYSRNDKSSWGVREEEGQSILFQPYKRFGAWEFGIE
                     FFYQKKHNDEFNHGKINDRSIYTETYYEPIIKYAFENAGNLYLRTRFGHGKTENADSL
                     QYNANRTYFKTIRKATLGYEQKIGDDWIAKIEYKHAWEKEHKNFYDPRDEEKYNVLNQ
                     DNIYVHAFYRF"
     gene            2267..3499
                     /locus_tag="ACHKNP_RS22145"
                     /old_locus_tag="pgaptmp_004429"
     CDS             2267..3499
                     /locus_tag="ACHKNP_RS22145"
                     /old_locus_tag="pgaptmp_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4706478.1"
                     /translation="MRLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLIWSDATSA
                     EYMKYAATEFNKDFGYKVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLV
                     VAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
                     FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKTGNDPKALGIASS
                     EAKKGLETMKRLKKANPSNPLDMGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINF
                     GVTRIPTLDGHQPRTFSTVRLAVVSSFTEYPHAAELFADYLTTDKMLMKRYEMTNLIP
                     PIDSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPD
                     EVLNHAQTIIEEQLSLQE"
     gene            3502..4791
                     /locus_tag="ACHKNP_RS22150"
                     /old_locus_tag="pgaptmp_004430"
                     /pseudo
     CDS             3502..4791
                     /locus_tag="ACHKNP_RS22150"
                     /old_locus_tag="pgaptmp_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            4794..5627
                     /locus_tag="ACHKNP_RS22155"
                     /old_locus_tag="pgaptmp_004431"
     CDS             4794..5627
                     /locus_tag="ACHKNP_RS22155"
                     /old_locus_tag="pgaptmp_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HGU4706479.1"
                     /translation="MDKFLRKISTLLVYLFLICNSILVLGPVIWTIMASFKKGNNLFS
                     STFSGIEFTFDHYITLFTDTPYMQWYLNTFILATANMLISLFVVTMTAYVFSRYRFRG
                     KRNTLMSILVLQMFPAFLSMTAIYILLSKANLIDTYTGLLLVYVTGSLPFMTWLVKGY
                     FDAIPTSLDEAAKIDGAGHLTIFIQIILPLAKPILVFVGLVLFTGPWMDFILPTLVLR
                     SEDKMTLAIGIFSWIASNSAENYTLFAAGALLVAVPITLLFVFTQKHITTGLVSGAVK
                     E"
     gene            5647..7446
                     /locus_tag="ACHKNP_RS22160"
                     /old_locus_tag="pgaptmp_004432"
     CDS             5647..7446
                     /locus_tag="ACHKNP_RS22160"
                     /old_locus_tag="pgaptmp_004432"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HGU4706480.1"
                     /translation="MITVSSLKHSAKSEDSYAYDNETLHVRLRTLRGEVDKVILWIGD
                     PYNWAEGGLDGGNMAGTEAFGWIGGNEIEMEQEAVTEFHDHWFAVFKPQKRRCRYGFI
                     LFGKEGEKFLFGEKRCVDISSPDCEERELSRLNNFFCFPYLNKIDVLNTPSWVKNTVW
                     YQIFPDRFCNCRPEISPEGVEPWGSAPTSFNFMGGDLWGVIDKLDYLEDLGINGIYFC
                     PIFTSASNHKYDTIDHFSVDPHLGGNIAFHTLIEEAHKRGIKIMLDAVFNHLGADSLI
                     WLDVVRNGANSRYADWFWIHKFPVYPDTPKSEWDFKNFNYETFGNVIEMPKLNTENEE
                     CREYLLSIVRYWTQNFDIDGWRLDVANEVDHHFWRDFRKVIKDIKPECYILGEIWHEG
                     TPWLRGDQFDSLMNYPLTYGIIDYFALQDTTKQEFMTSVTRSYLCYPKNITEVMFNLL
                     DSHDTARILSVCLNDKRKVKLAYLFMLTQAGSPCIYYGSEIGIDGFKSMTLENNRKCM
                     IWDENKQDLELRQFIRWLIRLRKKHPQWCVASIQWKDVEHPTVIAYQRDNITFFLNNS
                     EDTANFIYDGRSMEISGFSYEIEGLPAADLYDF"
     gene            7468..>8263
                     /locus_tag="ACHKNP_RS22165"
                     /old_locus_tag="pgaptmp_004433"
     CDS             7468..>8263
                     /locus_tag="ACHKNP_RS22165"
                     /old_locus_tag="pgaptmp_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HGU4706481.1"
                     /translation="MIIDAHTHIGDFVKIRMPEDVFLASLDKYNIDFALCSCGSAVEV
                     DHDQNPIPDEDQVTQHDNNERMLRLVRQHSKRIGAFMWIKPRLESCDQDFEDMIASNR
                     DIIYGIKVHPYHSKMAFNSDKVQEYIRLAQKYDLPVVSHTSNDYESSPQLVYEMALKY
                     PNVNFVMCHMGLATDNQEAIELIAKLPNLYGDTAWVRADMVYQSIKICGSDKILFGTD
                     NPINGLDTYNDDDFYNKYFTELKSKLTQSEFEQLMFTNAKKLFKIKI"
BASE COUNT         2425 a         1486 c         1639 g         2713 t
ORIGIN      
        1 tgctgtttag ttagtttatt aaggacagcg agatttttgt tgtattctcg cgtatttttg
       61 gcagggtaac caaaaaggtc actcttttca ggacaagaat ggcttacacc tgatttagcc
      121 ttaattaccg aataactacc aattgatatg tgaccggcga ttccgacttg accatgtgta
      181 ataacatggt cgcctaaaac tacgtgtcct gaaaaaccgc attgtgaaac aatcaggcat
      241 ttattaccga ttatgcagtc atgtccaatc tgcacttgat tatcaatacg agtgccttgg
      301 cctataatcg tatctccaag tgtccctcgg tcaatggtgt tgttacaacc gatttcaaca
      361 tcatcaccta tgattactct accgatgcta tcaactcgga catatgaatc acgttcatcc
      421 gacatatatt caaaactata attaccgatg gagttattag aatctatgat aacgtcattg
      481 ccaataatag taccttcttt aatcactgta ttcgcatgaa tggcaacatt atcacctatg
      541 gtcacacagt tcataatttt aacaccaggc ataaaatggc agttcatacc tatctggcag
      601 tgtttaccaa tgtatacatc aggtatcgtt gaagtgtttt cttgctcaaa caaacgatac
      661 ttatgtacct taaaatatct taaaactttg ttaagattat tcacgttcag gtaatcgact
      721 acaatctttg ccttagcatt agaactaatg atctcttccg gtccgataat tatatccgca
      781 ccagtcgttg gtatatgcaa caaatctcca ggtgtcataa caatcgagag tccaccaact
      841 aaaggactat ggattggccg aatggtatca accctgagtg atggatcacc atataaagtt
      901 ccttgcatca acgttttaat atctagtgcg ctgaacatag gtttccctct gtttaatcaa
      961 aagcgataga aagcatgaac ataaatatta tcctgattga gcacattata tttttcttca
     1021 tctcttggat cataaaagtt tttgtgctct ttttcccagg catgtttata ttcaatcttg
     1081 gctatccagt cgtcacctat tttttgttca taacctagag tggcttttcg aattgttttg
     1141 aaataggttc tgtttgcatt gtattgcaaa ctatctgcgt tttctgtttt cccgtgtccg
     1201 aagcgggtgc gtagatacag attacctgcg ttttcaaaag catatttgat aattggctcg
     1261 tagtaagttt cggtataaat actacgatca tttattttac cgtgattaaa ttcatcgtta
     1321 tgctttttct ggtagaaaaa ttcaatacca aactcccatg caccaaaacg tttatagggc
     1381 tgaaatagta tactttgacc ttcttcttcg cgtacgcccc aggagctctt atcatttcga
     1441 ctgtataagt attcaacgtt tgaataaaaa cctgcgttcc agtcagagtt aaagtacgtc
     1501 aacgtagggc gaaaactgtt gtcaacagtt acagtatgca agcctgtaac ataagggcta
     1561 tctatatttc cgtcggtata catcaggtcg tataccagac tgaagtcgaa accgttacca
     1621 atagtgaacg ttttgtttag tgatatatag ttttgcagac ttgtttcatt ttcgtgatag
     1681 cctttttctt cttgccggcg atcgatagac ttaaggccat agattgctgc aaattgccaa
     1741 ttaggttggc tgaaataggc ctggaaaatc tcgtgtgttt tagtatattc ctttactgtt
     1801 ttacctaaat agttatcagt gcttttggtc tcatattcaa atgtagtacc aacatgtcca
     1861 ttaaagcccg cttgttcttc tgaccagtca gtgtcggagg taaagccgcg atcgtttgcc
     1921 gcaaaggctc ctgttgagcc taaaaggact gttgcaattg aagccgataa gacattcttt
     1981 tttattaaaa ccataaatga gttccttcca aaatatcaat ttaagatatg atttctgtta
     2041 ttccatatag tcgtacctca tgtactccta aacactctgc ggcaataaaa tatttttttc
     2101 ctcaacaaat tactgcaaat gagatatgca tcactatttg cagttattca tcattaaaat
     2161 cttatttgac agttgattgg ccttgatttg tgttgataat ggcgtgaatg cttatgtagg
     2221 cgtacagcat gtactactta aagatattat tcacgaggta aatataatga gactttcaaa
     2281 tatcgtcaca gttataattc ttgcaatttc cagtacgcta acaccccagg cgatggcaga
     2341 aaaattgatt cctgaaactg atgctgagtt attaatctgg tctgatgcca caagcgcgga
     2401 atatatgaaa tatgcagcaa cggaattcaa taaagatttt ggctacaagg tcaagtttac
     2461 atttcgcaat atagcgccaa tggatgcagc atcaagaatt atgcaggatg ggggtacgac
     2521 tcgtgtagct gatgtagctg aaattgaaca tgataccctg gggcggttag tcgttgctgg
     2581 cggggttatg gaaaacatgg tctcagctga gcggattaaa aaaacattta ttccaggcgc
     2641 agtatcggca gctacatata ataacatcag ctatggtttt cctgtaagtt tcgcaacgct
     2701 ggcgcttttc tataataagg atttgttaaa caccgcacca aaaacattcg aagaaatcaa
     2761 tactttcagt gaaaagttta ataattcatc cgagcataaa tatgctctgc tatgggatgt
     2821 acaaaattat tatgtttcac gtatgtttat taccttgtat ggtgccaacg aattcggtaa
     2881 aaccggtaac gatcctaaag ctctaggcat cgcttcatct gaagcgaaga aagggctaga
     2941 gacaatgaaa cgcttaaaga aagcgaatcc ttctaatcct cttgatatgg gtaatccaca
     3001 agttctaaga ggtctgttta atgaaggaaa agttgctgct gtaatcgacg gaccttggtc
     3061 catacaaggt tacattgaca gcggaatcaa ttttggcgtg acacgcatcc caacattaga
     3121 tggtcatcag cctcgcacat tttcaacagt acgactggcc gttgtaagct catttaccga
     3181 atatcctcat gccgctgaat tattcgctga ctacctgaca acggataaga tgttgatgaa
     3241 gcgttatgaa atgacgaatc tcatcccacc aatcgattca cttatgaaca aaattagcca
     3301 gacaggtagt gaagctataa aagcaattat tgctcaagct aattattctg acgcaatgcc
     3361 atcaataccg gaaatgtctt atctatggtc tccaatgact aatgctattt tggctacctg
     3421 ggttgagaat aaaacaccgg atgaagtttt aaatcatgca caaacaatta ttgaagaaca
     3481 actttcgctt caggagtaat tgtgaataca caacaactga aaatgaagag tgcacctgtg
     3541 ctacctattt cctgtttgat tatgggcggg actcagttat cacgtcatta ttatgtgaaa
     3601 ggcgggatat tctttgcaat acaagtatgc tttttgcttt atctttccga tattgtgcat
     3661 acattaatag gtttgttcac attaggtgat gtcgcgcaaa ttcgaaaagg gttgactgtt
     3721 attcagggtg acaattctat atttatgctt gtagagggcg tgatagcgac cattattgtt
     3781 ggcttattcg ccactattta catccttaat attaaagatg cgcggaactc atcttattgc
     3841 catctgactt ttaaacaaca gttatataag ctctatgagg ataaatttgc atttatagtc
     3901 ctaacgcccg catttttagc gagtatagca ttcattgttt tacctattgt aattacagtt
     3961 cttgtatcgt ttacaaacta tgctgcgcca aatcacatcc caccaaagaa cctggttgat
     4021 tgggttggga ttaaaaattt cattatgttg tttaaattta aaatatggtc tgatacgttt
     4081 ctaggtgtcg ctctctggac atttatttgg gcaatttgcg cgacgatttt taccttcagt
     4141 tttggcttta ttctggcttt agcactggct aaaaaaaata tacgtttcgc aaaagtctgg
     4201 tgattgatat ttatactgcc atatgccata cccacatttg taacgttact tattttccgt
     4261 cttcttttaa atggaattgg tccggttaac tcgtttctga acagtatcgg aatgaattca
     4321 gttgattttc tgtcggttcc attaaatgca aaaataacag taattgccgt ttgtgtttgg
     4381 gtaggggcac cgtattttat gctattgatc gcgggtgcat taacaaacat atcccgagat
     4441 ttgtatgagg ctagtgaggt cgatggcgca actaaatggc aacagtttaa ggaaattaca
     4501 ttgccaatgg tgttacatca ggttgcgcca actctggtaa tgacatttgc atataatttc
     4561 aataattttg gtgcaattta tcttctgact gaaggtggtc cgactaatcc ggaatacaga
     4621 ttcgctgggc ataccgatat ttttattacc tggatttata aactcacatt gaatttccag
     4681 caatatcata tagcttctgt aatctccatc ataatattta tcttcttatc ggttatagca
     4741 atatggcaat tcagacggat gaaatcattt aaagatgatg tagggatgtg acaatggata
     4801 aatttttacg taaaatatca acgctcttag tttatctttt tttaatctgc aactccattc
     4861 ttgtgcttgg gccagtgata tggacgataa tggcatcttt taaaaaaggg aataatctat
     4921 ttagttcaac atttagtggc atcgagttta catttgacca ctacataacg ttgttcacgg
     4981 atacgccata tatgcagtgg tatttgaaca ctttcattct tgctaccgct aacatgttaa
     5041 tttcactgtt cgttgtgacg atgaccgcct acgttttttc aaggtatcga tttcgtggaa
     5101 agcgtaatac tttaatgagt attcttgtat tgcaaatgtt tcctgcattt ttatcaatga
     5161 ctgcaattta tatcctgcta tcaaaggcca atttgataga cacatatact gggttgcttc
     5221 tggtttatgt aaccggttca ttgcccttca tgacgtggtt ggtaaaaggt tattttgatg
     5281 ctattccaac ttctttggat gaggccgcta aaatagatgg agcagggcat ctgactattt
     5341 ttattcagat aatattgcca ttggctaagc cgattctcgt tttcgttgga ttagttttgt
     5401 tcactggacc atggatggac ttcatcttgc caacgttggt gcttcgtagt gaggataaaa
     5461 tgaccctggc gattggaatt tttagttgga ttgcttctaa ttcagcagag aattatacgc
     5521 tttttgctgc tggggcgctt ttagttgcag tacctatcac attgttattt gtattcacac
     5581 agaagcatat aactactgga cttgttagcg gtgccgttaa ggaataattt ttagggatta
     5641 gtatttatga ttacagttag ttcgttaaaa cactcagcaa aaagtgaaga tagctatgcc
     5701 tatgataacg aaactttgca tgtacggtta cgcacattaa gaggagaagt tgataaagtt
     5761 attctgtgga taggcgatcc atataattgg gctgaaggtg gtcttgacgg cggtaacatg
     5821 gccgggacag aagcgttcgg ctggattggc ggcaacgaga tcgagatgga acaagaagct
     5881 gtcaccgaat ttcatgatca ctggtttgca gtgttcaaac ctcaaaaacg ccgttgccgt
     5941 tacggtttta tactttttgg gaaagaggga gagaagtttc tttttggtga gaaaaggtgt
     6001 gttgatatca gttcaccgga ctgcgaagaa cgagagttaa gccgcttaaa taactttttt
     6061 tgcttccctt atcttaataa aattgacgtt ctaaataccc cctcctgggt aaaaaatacg
     6121 gtatggtacc aaatttttcc agaccgtttc tgtaactgca gacctgaaat atccccagaa
     6181 ggggtagaac cgtggggcag tgcaccgact tcattcaact ttatgggtgg ggatctgtgg
     6241 ggggttattg ataaactcga ttatctggaa gatctgggta ttaatgggat ttatttttgt
     6301 cctattttta cctcagcttc caaccataaa tatgacacca tcgatcattt tagtgttgat
     6361 ccacatttag gtgggaatat tgctttccat actttgattg aagaagcgca taagcgtgga
     6421 ataaaaatca tgttggatgc agtattcaat catttaggcg ctgactctct aatatggctt
     6481 gacgttgtac gcaatggtgc caattcacgt tacgccgatt ggttctggat tcataaattt
     6541 cctgtttatc ccgatactcc aaaaagtgaa tgggatttta aaaactttaa ctatgaaacc
     6601 tttggcaatg taattgaaat gcccaagcta aatactgaga atgaagagtg ccgtgaatac
     6661 ttacttagta tagttcgcta ttggacgcaa aattttgata ttgatggttg gcgtctggat
     6721 gttgcgaatg aagtggatca tcatttctgg cgagatttca gaaaggtgat taaagatatc
     6781 aaacctgaat gttatatttt gggtgagata tggcatgagg gcaccccctg gttgcgtggt
     6841 gatcagtttg attcattaat gaattatccc ttaacatatg ggattattga ttattttgcg
     6901 ttacaggata caacaaaaca agaatttatg acatccgtta cacgttctta tctttgttat
     6961 cctaaaaata taactgaagt tatgtttaat cttctggata gtcatgacac cgcgcgtatt
     7021 ttaagcgttt gtttgaatga taaacgaaaa gttaaactcg cctatttatt tatgttaact
     7081 caagcgggct ctccttgtat ctactacggt agcgaaatag gaattgatgg ttttaaatca
     7141 atgacgcttg agaataatag aaaatgtatg atatgggatg aaaacaaaca agatcttgaa
     7201 ttacggcaat ttatacgttg gttaatacgt ctacgtaaaa aacatccaca atggtgtgtg
     7261 gcatcaatac aatggaaaga tgttgaacat cctacggtga tcgcttatca acgcgataac
     7321 atcactttct tcttaaataa tagtgaagat actgccaact ttatttatga cggaagaagt
     7381 atggagatct ccggtttcag ttatgaaatt gaaggtttac ctgctgctga tctttatgat
     7441 ttttaaattt caattaggat gtttgttatg ataattgatg cacacactca tattggtgac
     7501 tttgtgaaaa tccgtatgcc agaagatgtt tttctggcat ctctggacaa gtataatatt
     7561 gattttgcat tatgttcttg tggatctgca gtagaagttg accatgacca gaacccgatt
     7621 ccggatgaag atcaagttac acagcatgat aataatgaac gtatgctcag acttgttcgt
     7681 caacattcaa agcgaattgg cgcgtttatg tggattaagc ctcgtctgga aagttgtgat
     7741 caagattttg aagatatgat tgccagcaat cgagatatca tttatggcat caaagttcac
     7801 ccatatcatt caaaaatggc tttcaattca gataaagttc aggaatatat acgccttgct
     7861 caaaaatatg atctcccggt agttagccat acttctaatg attatgaatc cagcccacag
     7921 ttggtttatg agatggcact taaatatcca aatgtgaatt ttgttatgtg tcatatgggg
     7981 ctcgcgacag ataatcagga agcgatcgaa ttgattgcaa aacttcctaa tctctatggc
     8041 gatactgcgt gggttagggc ggatatggtt taccagtcga taaaaatctg tggtagtgat
     8101 aagattttat ttggtaccga taatcctatt aatggactag atacttataa tgatgatgat
     8161 ttttataata agtattttac tgaattaaaa agtaaattaa cgcaaagtga gtttgaacaa
     8221 cttatgttta ccaatgcgaa aaaacttttt aagataaaaa tat
//
LOCUS       DAZFWL020000178         8244 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFWL020000178 DAZFWL020000000
VERSION     DAZFWL020000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.178"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1495
                     /locus_tag="ACHKNP_RS22170"
                     /old_locus_tag="pgaptmp_004434"
                     /pseudo
     CDS             1..1495
                     /locus_tag="ACHKNP_RS22170"
                     /old_locus_tag="pgaptmp_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1464..3200)
                     /gene="aceK"
                     /locus_tag="ACHKNP_RS22175"
                     /old_locus_tag="pgaptmp_004435"
     CDS             complement(1464..3200)
                     /gene="aceK"
                     /locus_tag="ACHKNP_RS22175"
                     /old_locus_tag="pgaptmp_004435"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HGU4706482.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPHFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3481..4785)
                     /gene="aceA"
                     /locus_tag="ACHKNP_RS22180"
                     /old_locus_tag="pgaptmp_004436"
     CDS             complement(3481..4785)
                     /gene="aceA"
                     /locus_tag="ACHKNP_RS22180"
                     /old_locus_tag="pgaptmp_004436"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HGU4706483.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4815..6416)
                     /gene="aceB"
                     /locus_tag="ACHKNP_RS22185"
                     /old_locus_tag="pgaptmp_004437"
     CDS             complement(4815..6416)
                     /gene="aceB"
                     /locus_tag="ACHKNP_RS22185"
                     /old_locus_tag="pgaptmp_004437"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HGU4706484.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6685..7614)
                     /gene="metA"
                     /locus_tag="ACHKNP_RS22190"
                     /old_locus_tag="pgaptmp_004438"
     CDS             complement(6685..7614)
                     /gene="metA"
                     /locus_tag="ACHKNP_RS22190"
                     /old_locus_tag="pgaptmp_004438"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGU4706485.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7771..8214
                     /locus_tag="ACHKNP_RS22195"
                     /old_locus_tag="pgaptmp_004439"
     CDS             7771..8214
                     /locus_tag="ACHKNP_RS22195"
                     /old_locus_tag="pgaptmp_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU4706486.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2120 a         2035 c         2036 g         2053 t
ORIGIN      
        1 tgaattgttt tataaagaac tcgaggataa attttctggc aaggagctgg cgacgcctct
       61 attaaaaagc ttctcagaga actgtagaca aaatggtcgt catatcttta gcaacaagga
      121 ttttgtcatt aaattttcca cgtccgtctt acaagctgat aagaaagaaa ttacgataat
      181 taataaaaac gaaaacacga cacttactca aaccattgcc ccaatatttg aagaatacct
      241 aatggaaatt ttacctcaac gctcagacac tcttgataaa caagaattaa acctaaaatc
      301 agatagaaaa gaaaaagaat tcccaagaat taaacttaat ggtcaatgtt attttccggg
      361 gcgaccccaa aaccgtatag tatgccgaca cattgctgca caatatatta atgatattta
      421 tcagaatgtt gattacaaac cccatcaaga tgattactct tcagctgaaa aatttctcac
      481 tcacttcaac aaaaaatgca aaaaccagac tttggcgttg atttccagcc gtcctgaggg
      541 gcgttgtgtt gctgcctgtg gtgatttcgg gctagttatg aaggcatatt tcgacaagat
      601 ggaatcaaat ggcatcagtg ttatggcagc catattactg gtggataacc atgctttgac
      661 ggtccggcta agaataaaga acacaactga aggatgtacc cattacgtgg tttcggttta
      721 tgatcctaat gtcactaacg ataaaataag aattatgagc gaaagcaaag aggatattaa
      781 acactattct ctgatggatt ttatgaatgt agattatagc ctcctgaaat ggtcaaatga
      841 tcatattatt aaccaatctg ttgcaataat tccagcactt ccgaaagaac agctattgat
      901 gttaaaagga tctgtggatg aaataacccc tccattatca ccagcaacga tgaatttgct
      961 aatggcaatt ggtcagaatc accaacttac gcaactgatg attcagctcc agaaaatgcc
     1021 agaactacat agaacagaaa tgttgactgc ctataatagt ggacatatga acgttattaa
     1081 tactattttt aacgcataac ccactctgtt taatacgttt aaattcgata aaaaaaatat
     1141 gaagcccctc ctcctggcaa ataattctaa tgaatatccc ggtttgtttt cagcgataca
     1201 gcataaacaa caaaatgttg tagagacggt ttatcttgct ttatctaacc atgcacgcct
     1261 gtttggattt accgctgaag atattatgga tttttggcaa cacaaagccc cacaaaaata
     1321 ctctgccttt gagttggctt ttgaattggg tcaccgggtt attgctgaat taatccttaa
     1381 tacattaaat aagatggctg aaagctttgg ctttacggat aaccctcgat acattgcgga
     1441 gaaaaattat atggaagctt tactcaaaaa agcatctccc cataccgtac gctaaatctt
     1501 tgcctgcgcc gatacgcata aacatcttcc acatgccctt cacgtatgcg gttttgtagt
     1561 gcgcgccagt aatcagcgcg gaacaggtcg gcgtgcatct cttcaaacag cggaccaata
     1621 cgcgggtcgg cgcatagcca gtggcgaaac tcttccggga aaacatcgcc cggcgagacg
     1681 ctgtaccacg gttcgctggc aagttcgtct tccggatagc gcggcggcgg gatgtcgcgg
     1741 aaattcactt ccgtcatgta gcaaatttca tcgtaatcat aaaaaaccac acgcccgtga
     1801 cgggtgacac cgaagttttt aaacagcatg tcgccaggaa aaatgttagc agcggcaagc
     1861 tggcgaatag cgttaccgta ttcttcaatg gcgtcgcgca actgctgacc ttccacttgt
     1921 tccagccaga tattgagcgg caccatccgc cgctcgatat agagatggcg aattacaatc
     1981 tgctcaccaa gatcggtgat tttttccgct gcttcctgga gcagtaattc cattaatgcc
     2041 ggggaaatat gccgcttctc cagcacaaag ttttcaaact cctgggtgtc cgccattcgg
     2101 cccacacggt cgtgctcttt caccagttga tagcaggcac gaacgtgagc ggcagacatc
     2161 tctttctgcg gcgcgaactt gtctttgatg actttgaata cccgatcaaa gcccggcagc
     2221 gtaaatacca acatcaccat tccacgaata cccggcgctt caatgaactg ctcattacag
     2281 ccctgtagat aaacgagata ttcgcggtag ctttcggttt tggcgtgctt ctggcagccg
     2341 atagccatat acaattcagc agtggtttta cccggcagaa tttcccgtag ccactcgacc
     2401 agcgctgcgg gcagcggtgc ataaaccata aaataagaac gcgcaaagcc aaaaacaatg
     2461 ctcgcttcgg cggtcgtcgt caggcaggta tcaataaata actcgccgtc gtccgtctgg
     2521 tggatcggca gtaagaaggg caacgtgccg gaaggcgtga tcagtttgcc caccagccaa
     2581 gcggctttat tgcggtaaaa cagttcgttc gccacctgta aatgactttc cgcgaggttg
     2641 tctgtcccca gcgtttccgt cagatggcga atgatgtaat gaatgtcacg gcttttattc
     2701 tgccagcgca ggcgcagcgg caggtcgctg ataacgcgca tcagtagaga ttcccagccg
     2761 tgatcggggt gaaagtcttt cgccagcggg cggggaatgg tacgaaagcg gcgctctggc
     2821 tgagagctaa agataaaaag ccgctcggga gtaagcgagc ggtggtcaaa taaccgacag
     2881 tacacggagt taaaaaagct ctccgcaatc tcgaagtgcg ggtaatccgg caacagccgc
     2941 gtgtaatgct ctttaacgcg cagtaaaaat gccgcgtccg tactttggcc gttagtaatg
     3001 cagcgcagtt gctccacgac cagaccaacg tgatgatcgt aaagatggat acggtttttc
     3061 atcgcctgct ggacagcatg ccagtcggcc tgttcgaaac gctgctgcgc cccggaagtc
     3121 acttcgagga atcgaccata ctgagcgtcg aagccttgca aaatggtttg agcaatcagt
     3181 aattccaggc cacgcggcat ttagcgccct catcaggagc agagaattgc cggatgcggc
     3241 gtaaacgcct tatccagcct acgttcggca acggttgtag gcatgataag acgcgcaagc
     3301 gtcgcatcag gcatcgtgca ccgattgccg gatgcggcgt aaatgcctta tccggcctac
     3361 gttcggcaac ggctgtaggc atgataagac gcgcaagcgt cgcatcaggc atcgtgcacc
     3421 gattgccgga tgcggcgtga acgccttatc cggcctacag tcagcaacgg ttgttgttgc
     3481 ttagaactgc gattcttcag tggagccggt cagcgcggtg actgaagacg tgccgccctg
     3541 aataatagtc gtcactttat cgaagtaacc tgtacccact tcctgctggt gagatacgaa
     3601 ggtatagcca tctttcgcgg cggcaaattc cggctgctgc actttctcaa cgtagtgctt
     3661 cataccctcg ccctgggcat aggcgtttgc caggtcaaac atgttgaacc acatgctgtg
     3721 gatacctgcc agggtgatga actggaactt gtagcccata tccgacagct gctgctggaa
     3781 gctggcaata gttttgtcgt cgaggttttt ctgccagttg aacgacggcg agcagttata
     3841 agccagcagt ttgcccggat atttcgcgtg gatagcttgt gcaaagcgac gcgccagttc
     3901 cagatccggc gtggaggttt cacaccagac caggtcagca tatggcgcat acgccaggcc
     3961 acggctgatc gcttgctcaa tgcccgcatg agtacggaag aagccttcac tggtacgctc
     4021 gccagtaata aattcactgt catacgggtc gcaatcggag gtaatcagat ccgccgcatc
     4081 ggcatcggta cgagcaacca gcagggttgg aacgcccgtc acgtcagctg caagacgcgc
     4141 cgcgaccagt ttctgaatag cttcctgagt tggcaccaaa actttgccgc ccatgtgacc
     4201 gcatttcttc actgacgcca gctgatcttc gaagtgaact gccgctgcac cggcttcaat
     4261 catcgctttc atcagttcaa aggcattcag gacaccgcca aaaccggctt ccgcatcggc
     4321 aacgatcggc aggaagtaat cgacatagcg cggatcgccc ggctcaatgc ctgcggacca
     4381 ttggatctga tcggcacgac ggaaggtgtt gttgatccgc tccaccacag ctggcaccga
     4441 gtttgccgga tagagcgact gatccggata catgctggcc gccaggttag cgtccgccgc
     4501 tacctgccat cccgacagat agactgcttc aatacccgct ttcgcctgtt gcagcgcctg
     4561 accgccagtc agtgcaccga ggctgttgat gtagcctttt ttcgactcgc catgcagcag
     4621 acgccacatt ttcgccgcgc ccagttgcgc cagggtgcat tcaggattga ctgaaccgcg
     4681 taatttcacc acatcttccg cgctgtatgg gcgagtaatg ccttcccaac gcggttgagt
     4741 ccactctttc tgtaattctt caatttgttg tgtacgggtt ttcatgtgca gatgctccat
     4801 agttatgtgg tggtttacgc taacaggcgg tagcctggca gggtcaggaa atcaattaac
     4861 tcatcggaag tggtgatctg ttccatcaag cgtgcggcat cgtcaaaacg cccctgggag
     4921 aaacgttctt cgcccagttc gctggcaatg actttcatct cttcacccag catctggcgg
     4981 aacaaggctt tggtcaccgg tttgccattg ctcaacgttt tttgatgatg gatccactgc
     5041 cagattgagg tacgggaaat ttcagccgtc gccgcatctt ccatcaggcc ataaatcggc
     5101 acgcagccgt tgccggagat ccatgcttcg atgtactgca ctgccacgcg aatgttggcg
     5161 cgcatacctt cttcggtgcg ttcaccatca caaggtgcca gcagctgatc ggcagtaatc
     5221 ggcgcgtctt gttcgcgcat cacttcaagc tgatttttac gggagccgag aatgtcgttg
     5281 aataccgcca ttgccgtatc cgcaaggcct gggtgagcaa tccatgtgcc atcgtgaccg
     5341 ttattggctt ccagcgcttt atccgctttt actttgttga gcacctggtt attgcgctct
     5401 tcatctttgc tcggaataaa cgctgccatg ccgcccatcg caaaagcacc gcgtttatgg
     5461 caggttttaa tcaacaggcg tgagtaagca ttcaggaatg gtttatccat cgtcactgcc
     5521 tgtctgtctg gcaggacgcg atcgggatag tttttcaacg ttttgatata gctgaagatg
     5581 taatcccaac gaccgcagtt cagaccaaca atatggtcac gcagcgcgtg aaggatttca
     5641 tccatctgga acacggcggg cagcgtttca atcagcaacg tcgccttgat ggtgccgcgc
     5701 ggcagattaa agcgatcttc tgcatagctg aagacttcgc tccaccaggc cgcttcctgc
     5761 caggactggg ttttcggcag atagaaatag ggaccgctgc cctttgccaa cagtgcctga
     5821 tagttgtgga agaaatagag cgcaaaatca aacaggctac cggggattgc ctcaccacgc
     5881 caggtgacat gtttttccgg caagtgcaga ccgcgtaccc gacaaatcaa aaccgctgga
     5941 ttgggcttga gctgataaat tttgcctgct tcattggtat agctgatggt gccgttaacc
     6001 gcatcgcgca ggttaatttg cccgtcgatc actttgttcc agtctggtgc cagtgaatct
     6061 tcgaaatcgg ccataaagac tttcacattg gcgttcagtg cgttgatcac catcttgcgc
     6121 tcaaccgggc cagttatctc gactcgacga tcttgtaagt ccgcgggaat accacgaatt
     6181 ttccagtccg tatcgcgaat ggaagctgtt tccgaaataa aatcaggcaa agttccgtta
     6241 tcaatatctt gctgctgctg aatgcgcgct gccagaagtt tattgcgttg tggcgtaaaa
     6301 tgcgtcacca gctcagtcag aaattctacc gcttcggcag taagaatttg cttctcctgc
     6361 tcgccatacg gccttatgaa agccagttca tcggttgttg ttgcctgttc agtcatcgtg
     6421 cagctcctcg tcatggaccc gaaaacttcc ccactgtgaa cgaaggatcg ttgtgcgctt
     6481 ttcgttcagc acaactaaga ctactcattt aaaatgcaaa atcaaaaaca atttccattt
     6541 taattaaaaa tacttgataa cttattgata acaatatgaa taaaatttat tcacctcctg
     6601 aggtgcgttt cggaaataaa aaaggcaccc gaaggtgcct gaggtaaggt gctgaatcgc
     6661 ttaacgatcg actatcacag aagattaatc cagcgttgga ttcatgtgcc gtagatcgta
     6721 tggcgtgatc tgatagacgt aatagttgag ccagttggta aacagcaaat taccgtgact
     6781 acgccagctc gctcgcggtg tattttgcgg atcattgtgc gggaaatagt tatacggtac
     6841 ttccgggcct agtccggctt ccacatcgcg gaaatattcc tgcgccagcg tttgcgcatc
     6901 atattcggga tggcccgtca caaaggcaat gcgcttatct ttgctggcaa acagatatgc
     6961 atccccttct tccgtctctg ccagaatttc cagatcggtg taatcacgaa tcaacgctgc
     7021 cggaaagtca gcatagcgcg aatgtggtgc caggaatgaa tcatcaaagc cacgcgtcag
     7081 aagcgcatga ggatggagaa tatgatgctc gtaaacgcca gagagtttgt cggtgcgagt
     7141 ttgcttagga atgccgtaga ggatattgag cgcggcctgt accgcccagc agacaaacag
     7201 cgtcgaggtg acgtgatctt tcgaccactc cagcacctgt ttgatctgcg gccagtaagc
     7261 gacatcatta aactccacca ggcccagcgg cgcaccggtt acaatcagac cgtcaaagtt
     7321 ctgttcctga atatcttcaa agttacagta gaagttgttc agatgctctg cgggcgtgtt
     7381 gcgcgattca cgggaatcga tgcgcaacag ctgaatatcg acctgcaaag gtgagtttga
     7441 aagcaggcgc agaaactgat tttcagtttc aatcttcttc ggcatcaggt taaggattag
     7501 aaccttaagt ggacgaattt cctgaccaga cgcacgagaa gttgtcatca caaagacgtt
     7561 ttcttcacgc aagaaattga cggcgggtag ctcgtccggc acacgaatag gcataacctg
     7621 attacctcac tacatacgct tatacgttta gacatccaga tagctgaaga taaccagaaa
     7681 atttgccaat gtcgagcctg catgttgaag gtgagaaagt ttcaaggaaa aggctacgtt
     7741 agaatataag aatgacgaaa aggagagaaa atggttatta gtattcgccg ctcacagcat
     7801 gaggaagggg aggaactcgt tgcgatttgg tgtcgatctg tcgatgccac ccacgatttt
     7861 ttatcagcag agtatcgggc cgagctggaa gagctggttc gttcctttct gccggaagca
     7921 ccgttgtggg tcgcggttaa tgaacgggat cagccggttg ggtttatgtt gttaagtggg
     7981 cagcatatgg atgcgctgtt tatcgatcct gatgtgcgcg gctgcggcgt gggtcggatg
     8041 ctggtggagc atgcgctctc gatggcaccg gaactgacaa ccaacgttaa tgagcaaaat
     8101 gagcaggcgg ttgggttcta taagaaggtg ggttttaagg ttacgggacg ctctgaggtg
     8161 gacgatttgg ggaaaccgta tccgttgctg aatctggcgt atgtggggga ataagccgcc
     8221 cctgccgtgc ctgcccaggc tgga
//
LOCUS       DAZFWL020000179         8237 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFWL020000179 DAZFWL020000000
VERSION     DAZFWL020000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.179"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            112..723
                     /gene="ruvA"
                     /locus_tag="ACHKNP_RS22200"
                     /old_locus_tag="pgaptmp_004440"
     CDS             112..723
                     /gene="ruvA"
                     /locus_tag="ACHKNP_RS22200"
                     /old_locus_tag="pgaptmp_004440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGU4706487.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            732..1742
                     /gene="ruvB"
                     /locus_tag="ACHKNP_RS22205"
                     /old_locus_tag="pgaptmp_004441"
     CDS             732..1742
                     /gene="ruvB"
                     /locus_tag="ACHKNP_RS22205"
                     /old_locus_tag="pgaptmp_004441"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGU4706488.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1889..2674)
                     /gene="znuB"
                     /locus_tag="ACHKNP_RS22210"
                     /old_locus_tag="pgaptmp_004442"
     CDS             complement(1889..2674)
                     /gene="znuB"
                     /locus_tag="ACHKNP_RS22210"
                     /old_locus_tag="pgaptmp_004442"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HGU4706489.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(2671..3426)
                     /gene="znuC"
                     /locus_tag="ACHKNP_RS22215"
                     /old_locus_tag="pgaptmp_004443"
     CDS             complement(2671..3426)
                     /gene="znuC"
                     /locus_tag="ACHKNP_RS22215"
                     /old_locus_tag="pgaptmp_004443"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HGU4706490.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3505..4437
                     /gene="znuA"
                     /locus_tag="ACHKNP_RS22220"
                     /old_locus_tag="pgaptmp_004444"
     CDS             3505..4437
                     /gene="znuA"
                     /locus_tag="ACHKNP_RS22220"
                     /old_locus_tag="pgaptmp_004444"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HGU4706491.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4453..5775
                     /gene="mepM"
                     /locus_tag="ACHKNP_RS22225"
                     /old_locus_tag="pgaptmp_004445"
     CDS             4453..5775
                     /gene="mepM"
                     /locus_tag="ACHKNP_RS22225"
                     /old_locus_tag="pgaptmp_004445"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HGU4706492.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQATQEDEAIPLDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5895..6866
                     /gene="lpxM"
                     /locus_tag="ACHKNP_RS22230"
                     /old_locus_tag="pgaptmp_004446"
                     /gene_synonym="msbB"
     CDS             5895..6866
                     /gene="lpxM"
                     /locus_tag="ACHKNP_RS22230"
                     /old_locus_tag="pgaptmp_004446"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU4706493.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6955..>8237)
                     /locus_tag="ACHKNP_RS22235"
                     /old_locus_tag="pgaptmp_004447"
     CDS             complement(6955..>8237)
                     /locus_tag="ACHKNP_RS22235"
                     /old_locus_tag="pgaptmp_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HGU4706494.1"
                     /translation="KIISDAQSTLLSKIDTSSYKDPHILFNTTCFARNEWININNNWL
                     KARVNSYGYAVIDPKNKIVNGLKAESRSIENNYIKLLFSESGDLISLYDKRYGKEYIT
                     ENMHSEIRVYHEDAGFFAAWDFASNYRDGESYVLLAEKMTTVISGPKTTMTLIYHYNS
                     SYLRFAFTLTQDSPRVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQFGVIDRPTH
                     SDDSFAFAKDEIPAHHWVDLSDQETGIALIAKNKYGYRVKDQTLELTLLRSQHKPGEV
                     VKTDNYDNFLINNFADIGKQKFTFSLFPHHGDYRVGGVVNQAYIMNHPLQIVPVKPHP
                     GELPPSLSFFAIDTNSVIIETIKLAEDGDGIIVRLYESYGLEISAMFSWVCNYHYVQY
                     VDLQENKLDDSFYCNQYCNLEFKPFEIITLRLTQ"
BASE COUNT         2199 a         1941 c         2065 g         2032 t
ORIGIN      
        1 ttttcagatc gttgggacgc ctcgcaagtt ttcttcatcc ttcgctggat atctatccag
       61 cattttttta tcatacagca ttatctttga ttcattacgc aggagcgtca tgtgataggc
      121 agactcagag gcatcatcat tgaaaaacaa cccccgctgg tgttaattga agtgggcggc
      181 gtaggctatg aagtgcatat gccgatgacc tgtttttatg aactccctga agcgggtcag
      241 gaagcgatcg ttttcaccca ctttgtggtg cgtgaagacg cgcaactgct gtacggtttt
      301 aacaataaac aagagcgcac attgttcaaa gagttgatca aaaccaacgg cgtcggcccg
      361 aagttggcgc tggcgatcct ctccggaatg tcagcgcagc agttcgttaa tgccgttgag
      421 cgtgaagaag tgggggcact ggtgaaactg ccgggtattg gcaaaaaaac cgccgaacgc
      481 ttgattgttg aaatgaaaga ccgatttaaa ggtttgcatg gcgatctctt tacgccagcc
      541 gccgacctgg tactcacgtc acctgccagc ccgacgaccg acgatgctga acaggaagcg
      601 gttgccgcgc tggtggcgct gggctataaa ccacaagaag caagccgcat ggtgagcaaa
      661 atcgctcgcc ctgacgccag cagtgaaaca ttaattcgcg aagccctacg cgccgcgtta
      721 tgaggtaaag gatgattgaa gcagaccgtc tgatttctgc cggtaccact ttgccggaag
      781 atgtggcaga tcgcgccatt cgccctaaat tactggaaga gtatgttggt cagccgcagg
      841 ttcgttcaca gatggagatt ttcatcaaag cagcgaaact gcgcggcgat gccctcgatc
      901 atctgttgat tttcggtcct ccggggttgg gtaaaactac gcttgccaac attgtcgcca
      961 atgaaatggg cgttaattta cgcacgactt ctggtccggt gctggaaaag gcgggcgatc
     1021 tggcagcgat gctcactaac cttgaaccgc atgacgtgtt gtttattgat gagatccacc
     1081 gtctttcgcc agtggtggaa gaggtgttgt atccggcaat ggaagactac caactggata
     1141 tcatgattgg tgaaggtccg gcggcacgct ccattaaaat tgatttgccg ccgtttaccc
     1201 tgattggtgc aaccacgcgc gcaggttcgc tgacatcacc gttgcgcgat cgttttggta
     1261 ttgtgcagcg tctggagttt tatcaggtgc cggatctgca atatatcgtc agtcgcagcg
     1321 cacgctttat ggggcttgag atgagtgatg acggcgcgct ggaagttgct cgtcgagctc
     1381 gcggtacgcc gcgcattgcc aaccgtctgc tgcgtcgagt gcgtgatttc gccgaagtga
     1441 agcacgatgg caccatctcg gcagatatcg ctgctcaggc gttggatatg ttgaatgtcg
     1501 atgctgaagg tttcgattat atggaccgca agttattgct ggcggtaatc gataagttct
     1561 ttggtgggcc ggtaggtctg gataaccttg cggcagccat tggcgaagaa cgtgaaacca
     1621 ttgaggatgt gctggaacct tatttgattc agcaaggctt tttgcagcgt acaccgcgtg
     1681 ggcgtatggc gacgacgcgg gcgtggaatc actttggcat aacgccgcca gaaatgccgt
     1741 aagtcggatt ggtcgctcgc gccgcatccg acactggcag tgtgtctgat gcgacactgt
     1801 tcgcgtctta tcaggccagc cagagatagc gaaccgtagg tcggataagg cgctcgcgcc
     1861 gcatccgaca aatgtgttca gcgatagatt agctggcctg ctttttcatc atactgagaa
     1921 taaataacag tgccgcacat agcaccaccg acggacccgc cggcgtatcg taaaacgcgg
     1981 aaaaggttaa accgccagtt actgccacca tccccaccaa aacagcgaca ccagccatct
     2041 gttccggcgt acgggcaaag cgacgcgcag tagctgcagg aataatcagc agtgaagtaa
     2101 taatcaacgc gccaacaaat ttcatcgcta caccaatcgt caatgccgtc accagcatca
     2161 acaacaattt cacgcgctgt aatttcacac catcaacaaa cgccagatcc gggctaatcg
     2221 tcatcgacag caaattgcgc cattgccaga acaaaatagc caccacgatg accacgccaa
     2281 tcgcaatagc gatgagatct tctggcgtca ctgccagcaa atcaccgaac aggtaagcca
     2341 tcaaatcaac acgaatgtta gacatcagac taacgaccac caggcccagc gacagggcac
     2401 tgtgcgccat aatccctaat aacgtgtcaa tcgccagctg tggacgcttc tccagccata
     2461 ccagaccgcc cgccaggagc agcgtaacgg caattaccgc atagaatgga ttcacgtcca
     2521 gcaacaaacc aaacgcgacg ccaagtaatg aggcatgagc cagcgtatca ccgaaataag
     2581 acatacgacg ccagactaca aacgaaccca gcggacccgc ggcacaggcg agcatgatcc
     2641 cggctaacca accgggaaat aataattcaa tcatgagcga tcatttcccc gacgcaaaac
     2701 aattcgtccc tgtaaatcgt gacgatgatt atgatgatgg cgatagatac ccagttgttc
     2761 agcaccacga ggaccaaaca tagaaataaa ctccggatgt agggaaacaa cttccggtgt
     2821 gccggaacaa caaatgtggt gattcagaca cagcacttca tcggtttttg ccattaccag
     2881 atgcagatcg tgagagacca ttaaaacgcc acaatccagt tcgcgacgca actggtcaat
     2941 aaggtcatat aacgccacct ggccatttac atccacgcct tgagtgggtt catccagcac
     3001 taataattgc ggtcgattta acaatgctcg cgctaacagt acacgctgtg tttcgccacc
     3061 agagagcttt tgcatcggtg cgttaatcag atgcccggcc tggacacgtt tcagtgcagg
     3121 caaaatatct tctttatgtg tgccagggcg taagcgtaaa aaacggttta cggtcagtgg
     3181 caacgtggtg tcgagataca gcttctgcgg tacatagccg atgcgtagtt ttccgttgcg
     3241 cttgataacc ccttcatcgg gtgttaccag cccgagcact acccgtacca gtgtcgactt
     3301 acctgcgcca tttggcccaa gtaaagtcaa aatttttcca ggtttaagtt ccagcgacac
     3361 atcagagagg acgcggcgtt ggccaaaaga aaccgagaca ttttccaggg aaaccagact
     3421 tgtcatgtta attttagtct tgcagtagtc atgaaatgtt ataatatcac acttctcata
     3481 ttcattacga tgattggtcg cattatgtta cataaaaaaa cgcttctttt cgcagcatta
     3541 tccgccgctc tctggggagg tgcaacacag gccgcagatg ccgccgttgt cgcttcgctt
     3601 aaacccgttg ggttcatcgc ttctgccatt gctgatggtg taacagaaac ggaggtttta
     3661 ctgcctgacg gcgcttcaga acatgattat tcactgcgtc catcggatgt aaaacgctta
     3721 cagaacgcgg acttagtcgt ttgggttggc ccggagatgg aagcgtttat gcaaaaaccg
     3781 gtaagcaaat taccaggagc gaagcaggta acgattgcgc agcttgaaga tgtgaaaccg
     3841 ctactgatga aaagtattca cggcgatgat gatgatcacg accacgcgga aaaaagtgac
     3901 gaagatcacc atcacggcga tttcaacatg catctttggc tttccccaga gatagcgcgg
     3961 gctacagcgg ttgcaatcca tggaaaatta gtggaactta tgccgcaaag tcgagccaaa
     4021 cttgacgcca acctgaagga ttttgaggca caattagcct caaccgaaac gcaggttggt
     4081 aacgagctcg cgccgctcaa ggggaaaggt tatttcgttt ttcacgatgc ttacggctat
     4141 ttcgaaaaac agtttggact gacaccgctt ggtcatttta ccgttaaccc tgagattcaa
     4201 cctggcgcgc agcgtttaca tgaaataaga acacagttgg ttgagcaaaa agcaacctgc
     4261 gtttttgctg agccacagtt caggccagcg gtcgttgaga gcgtcgcacg agggacatcc
     4321 gttcgtatgg gaacgttgga tcccctgggg acgaatatca aactgggtaa aacaagctat
     4381 tcagaattcc tgagtcaatt agccaaccag tatgcgagct gcctgaaagg agattaatga
     4441 ggaagtgatt acgtgcaaca gatagcccgc tctgtcgccc tggcgtttaa taatttaccg
     4501 cgaccacacc gcgttatgtt ggggtcgctc accgttctta ctctggccgt cgctgtctgg
     4561 cggccttatg tttatcaccg tgatgccacg ccaattgtca aaaccattga gctggaacag
     4621 aacgaaattc gttcgctctt acctgaagcc agtgagccga ttgatcaagc tacacaagaa
     4681 gatgaagcca ttcccctgga cgaactggat gacaaaatcg ccggtgaagc gggcgtgcat
     4741 gaatatgttg tttccactgg cgatacgcta agcagcattc tcaatcagta tggtattgat
     4801 atgggtgata tcacccaact ggctgcggcc gacaaagaat tgcgtaacct gaaaatcggt
     4861 caacaactct cctggacatt aaccgcggac ggcgaactgc agcgcctcac ctgggaagtg
     4921 tctcgtcgtg aaacccgaac ctatgaccgt actgccgcta acggttttaa aatgaccagc
     4981 gaaatgcagc aaggagagtg ggttaacaat ctgctgaaag gtaccgtcgg ggggagcttt
     5041 gttgccagcg ccagaaacgc cggtttaacc agcgccgaag tgagcgcagt gattaaagcc
     5101 atgcagtggc aaatggattt ccgcaaactg aaaaaaggcg atgaatttgc ggtgttaatg
     5161 tctcgagaaa tgcttgatgg taaacgtgag caaagccagc tgctgggcgt acgtttgcgt
     5221 tcagaaggta aagattatta cgctatccgc gctgaagatg gcaaattcta cgatcgtaac
     5281 ggtactggtc tggcgaaagg attcttgcga ttcccgacgg cgaaacagtt ccgtatctcg
     5341 tctaacttta acccgcgtcg aactaatccg gtgacaggtc gcgttgcacc acacagaggt
     5401 gttgatttcg ccatgccaca aggtacgcca gtgctttcag tgggtgacgg tgaagtggtg
     5461 gttgccaaac gcagtggcgc agcaggttat tatgtggcta ttcgtcatgg tcgcagctac
     5521 accacgcgtt atatgcactt gcgcaagatt ctggtgaaac cgggacagaa ggtgaaacgt
     5581 ggcgaccgta tcgcgctttc cggtaatacc ggacgttcaa ctgggccgca tctgcactat
     5641 gaagtatgga taaaccagca ggccgtaaac ccgctgacgg caaaactgcc gcgtactgaa
     5701 gggctgaccg gctccgatcg tcgcgaattc ctggcgcagg ccaaagagat tgtgccgcag
     5761 ctacggtttg attaattaac atccattcgc agccggtacg cagtcagtac cggctttttt
     5821 tatttggtgc ggggcaagtt gcgccgctac actatcacca gattgatttt tgccttatcc
     5881 gaaactggaa aagcatggaa acgaaaaaaa ataatagcga atacattcct gagtttgata
     5941 aatcctttcg ccacccgcgc tactggggag catggctggg tgtagcagcg atggcgggta
     6001 tcgctttaac gccgccaaag ttccgtgatc ccattctggc acggctggga cgttttgccg
     6061 gacgactggg aaaaagctca cgccgtcgtg cgttaatcaa tctgtcgctc tgctttccag
     6121 aacgtagtga agctgaacgc gaagcgattg ttgatgagat gtttgccacc gcgccgcaag
     6181 cgatggcaat gatggctgag ttggcaatac gcggaccgga gaaaattcag ccgcgcgttg
     6241 actggcaagg gctggagatc atcgaagaga tgcggcgtaa taacgagaaa gttatctttc
     6301 tggtgccgca cggttgggcc gtcgatattc ctgccatgct gatggcctcg caagggcaga
     6361 aaatggcagc gatgttccat aatcagggca acccggtttt tgattatgtc tggaacacgg
     6421 tgcgtcgtcg ctttggcggt cgtctgcatg cgagaaatga cggtattaaa ccattcatcc
     6481 agtcggtacg tcaggggtac tggggatatt atttacccga tcaggatcat ggcccagagc
     6541 acagcgaatt tgtggatttc tttgccacct ataaagcgac gttgcccgcg attggtcgtt
     6601 tgatgaaagt gtgccgtgcg cgcgttgtac cgctgtttcc gatttatgat ggcaagacgc
     6661 atcgtctgac gattcaggtg cgcccaccga tggatgatct gttagaggcg gatgatcata
     6721 cgattgcgcg gcggatgaat gaagaagtcg agatttttgt tggtccgcga ccagaacaat
     6781 acacctggat actaaaattg ctgaaaactc gcaaaccggg cgaaatccag ccgtataagc
     6841 gcaaagatct ttatcccatc aaataaaaaa cagcctctca aattattgag aggctgtact
     6901 tgataaaaaa tctcaatgag tgttctcacc attttgagtt tttagattag tacgctattg
     6961 agttaatcgt agcgtgataa tttcaaaagg tttgaattca agattgcaat attgattgca
     7021 ataaaaactg tcatctaatt tattttcttg gagatcgaca tattgaacgt agtgataatt
     7081 acatacccac gaaaacatgg ctgaaatttc taatccataa ctctcatata atcttacgat
     7141 tataccgtcg ccgtcttcag ctaattttat tgtttcgatg ataacgctgt tagtatcgat
     7201 agcaaaaaat gatagtgaag ggggtagttc acccgggtgt ggtttgacag ggacgatctg
     7261 taagggatga ttcattatat aggcctggtt aacgacacca cccacacggt aatctccatg
     7321 atgaggaaat aaggagaatg tgaatttttg tttaccaata tctgcaaaat tattaatcag
     7381 gaaattatca taattatctg tcttaacaac ttccccaggt ttgtgctgcg aacgcaacaa
     7441 agttaactct agcgtctggt ctttgacacg atagccatat ttgttttttg ctattaaagc
     7501 aatgccagtt tcttgatcag aaaggtcgac ccagtgatgt gctggtattt catctttggc
     7561 aaaagcgaac gaatcatcgc tatgcgttgg tctgtcaatt acaccgaact gaatttgaca
     7621 ttgtgcaaga gatgtttgta ctgataccgg gaattttact ttgagagaaa cattaggttc
     7681 gtgccaatca ataaaagttt ggacatcaac gcgtggactg tcctgtgtca gggtaaatgc
     7741 gaagcgaaga taagaggaat tgtagtgata aatcagcgtc atcgtagttt taggcccgct
     7801 gatcacagtt gtcatttttt cagcgagtaa aacatatgat tccccatcac gataatttga
     7861 agcaaaatcc caggccgcga aaaaacctgc atcttcgtgg tatacacgga tctcactgtg
     7921 catattttca gtaatgtatt ctttaccata tcttttatca tatagcgaaa ttaaatcacc
     7981 gctttctgaa aaaagaagtt taatgtaatt attttcaata cttcgagatt ctgccttcag
     8041 accatttacg atcttattct ttggatcaat cacagcataa ccataagagt ttactcgtgc
     8101 tttgagccag ttgttgttta tattgatcca ttcgttacga gcaaaacagg tggtattaaa
     8161 taaaatgtgc ggatctttat aagatgaagt gtcaatttta cttaataaag tagactgcgc
     8221 atcagaaata attttct
//
LOCUS       DAZFWL020000180         8158 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFWL020000180 DAZFWL020000000
VERSION     DAZFWL020000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.180"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(122..461)
                     /locus_tag="ACHKNP_RS22240"
                     /old_locus_tag="pgaptmp_004448"
                     /pseudo
     CDS             complement(122..461)
                     /locus_tag="ACHKNP_RS22240"
                     /old_locus_tag="pgaptmp_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(777..1421)
                     /locus_tag="ACHKNP_RS22245"
                     /old_locus_tag="pgaptmp_004449"
     CDS             complement(777..1421)
                     /locus_tag="ACHKNP_RS22245"
                     /old_locus_tag="pgaptmp_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706495.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1442..1711)
                     /locus_tag="ACHKNP_RS22250"
                     /old_locus_tag="pgaptmp_004450"
     CDS             complement(1442..1711)
                     /locus_tag="ACHKNP_RS22250"
                     /old_locus_tag="pgaptmp_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4706496.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1790..2428)
                     /locus_tag="ACHKNP_RS22255"
                     /old_locus_tag="pgaptmp_004451"
     CDS             complement(1790..2428)
                     /locus_tag="ACHKNP_RS22255"
                     /old_locus_tag="pgaptmp_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HGU4706497.1"
                     /translation="MGDSVTPEILGNMIRQYFSQERPEEEAIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(2413..3636)
                     /locus_tag="ACHKNP_RS22260"
                     /old_locus_tag="pgaptmp_004452"
                     /pseudo
     CDS             complement(2413..3636)
                     /locus_tag="ACHKNP_RS22260"
                     /old_locus_tag="pgaptmp_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(4137..6818)
                     /locus_tag="ACHKNP_RS22265"
                     /old_locus_tag="pgaptmp_004453"
     CDS             complement(4137..6818)
                     /locus_tag="ACHKNP_RS22265"
                     /old_locus_tag="pgaptmp_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HGU4706498.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQVSVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKFIREKQVSLNEYEEVLKESDFKILLERLTSWSVLYLKQHLRRNISTRNSFSAET
                     YRDEFNRFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(7059..8105)
                     /locus_tag="ACHKNP_RS22270"
                     /old_locus_tag="pgaptmp_004454"
     CDS             complement(7059..8105)
                     /locus_tag="ACHKNP_RS22270"
                     /old_locus_tag="pgaptmp_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HGU4706499.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
BASE COUNT         2195 a         2082 c         1656 g         2225 t
ORIGIN      
        1 aaggctgggc catactcctc cggcagaagc agaaaaagct tattatgctt ccatcggaaa
       61 cgatgatctg gcagcctgag ttcacagata aaacactctc caggaaaccc ggggcggttc
      121 aaacctgatt ctggaaaagg actttgtgga ttgttgctat tgccagtgag attgaatgaa
      181 gtcgttttaa ctcgctttga atacgtcttg cccccagatt tcgctgagaa cgtaactcca
      241 gaatcagagc gacttcgcaa gtgccggttt tcgttgatgg tgaacgcttg gggcgacggc
      301 tgtggctttc cagaccagcg atgccctgag ccagatatct gcgccaccat tttacgcaat
      361 gtgggccgag aaatgccaca gcgacggcat acaaatcccg catcaccgga cgtttcatag
      421 agttttaccc actgtagccg ctgctgaatt tccctgttca taatttggca ttagttcagc
      481 tcaatcccga cttaaaaagt ccctacagga tgttccggac ctgaggcaca gttcatatcc
      541 ttatccataa aaaatttagc tatgtctata gcattaatct tgacaagacg acgttagggc
      601 ctggcgatga atcatttccc ctgccagata tcctttgggg tgcaccagaa ctaccgtttt
      661 atatctatat aactgaaacg tccctcgcca tccttcatat tttgatgtag taacaccatt
      721 cactttcatc taccccccgt tttattaaca tatagtaaaa atcacctcgc cgcattttat
      781 tcttttgaag taaaatcatt taccagttca cttaaagctc tcttttgttc atcatcatac
      841 agagattgaa tccggaattt aatctcttct gcaaccagtc ttgcatcggg ctcataaaat
      901 atttttgtta ttcgctgttt ctgatgttca ggctggtctt ccatatttcg taaaaccaga
      961 tgaaataata tatcataaag tgcgcaatgc catgaataac catcattggt taaaataagc
     1021 tcccaaaccc gctcctgctc ggcaataagc tgcagaacaa aggactcaac ctgttctgtt
     1081 tgttcggggg caacacggta tagcggagca atcacagcaa aagatagttc atttaaccag
     1141 gattccgctt ccttctcgct cctgaacggt ggacagtttt gtattcttcg ttcgtattca
     1201 cgggtaatct gctccagaat atcctccggc ggtgtcagcc acttattcct gagcaataca
     1261 ctggttgatg gatgacagct ggctatttca gcaataattt cacgtaatgt ctccgggctg
     1321 aacttcttca gctgattgta gtgctcccgg gtaaaaccat gttcatcgca taaaagttgc
     1381 catgtcattg atatactgtt tgccattcct tgcttcatca ttaatacctg ttacctgaat
     1441 atcaacagac cggtgtacct tttctgaata cctccccgga cggagtggca atccccattc
     1501 gcctcagcgt ggcatctgca gctttcgcca cctcgctgat atccagcgtt tcaggtagcg
     1561 gcggggacga tatctgcgcc agacaataat tccggcgcaa caacaaccac tcccgttgtg
     1621 cttctgtaca ttcaacattt cccataacaa aaagccagcg gtcagaagct ctgcgccagc
     1681 aaccagcagc ctccagtttc tgcgcaacca tgtcttccac aacttccggg gtactctttt
     1741 tcctgctcaa aacattaact cactgaaata atatacatat ctgacggtct cagcgaaccg
     1801 tccatgccgg gctgaaatcc tcttcctgaa acagctccgt cagcccgcta atctgtttca
     1861 atctgagaac ttcatcctgg tccataccca gctccgtacc aatgcgttca ttcgtccagc
     1921 ccaggcgcga caggtccctg acaatatcag acattgaggt tatctgatgt ttgcccctgg
     1981 cccggttatg tcggatggtc gcagcaatac gtgaggcctg tccctttctc tccggattaa
     2041 tacagaccac cggcagccag cccttcagac gttttcccgt acctgcttcc ctgcccagta
     2101 cctggcgatg aaaaccatcc accaccaagt aatggttctt ttcctcagac actaccaccg
     2161 gttgtgtgaa accatctttc tccagtgact gcttcagcag tttcttctca cccggcgcca
     2221 tgacattagg attgtaatca ttggccacca cctcatctgc ttttacccac agcacacagt
     2281 ccacaggctc ctgtgcaaaa ggactgacct catgaagcac cctgcgcagg tgattaaggg
     2341 cctgtatggc ctcttcttct ggtcgttcct ggctgaagta ctgacgaatc atgttgccaa
     2401 gtatctccgg tgtcacagaa tcccccattc cttcctcctt tccttcatac gctgcaggta
     2461 acgttcatag tgccggggtt tgttagggct aaaggagagg gtccggcacc agaaatcatt
     2521 ctttatgaga gtcttacaga tacgtcgcca ggacggaata tcccggctcc ccaggtcatt
     2581 ctcctgttca tccggaatgt catccgggaa gccccgcgtc tgataccaac gcaggtagac
     2641 agcaatttta ttacggtaat gttctttccg gcatcacatc cagcaggaac atcgcatagc
     2701 tgcgccaggt atgatgaggc ggcttcgtga tacgcttaca cagggcaaaa taggcaccac
     2761 tctcattggc ataaagcgcc ccgctggcag cccctgacac ccgttcacac atcctggccc
     2821 aggtttccgg ctccagaaca tggtaaagcc acagtccctt acgctgttca ggaccaaaag
     2881 gttcacagac acgcatgttg cgtaacggca cgccggcacg gtacatcagg tcatacagag
     2941 gattgtaaat ggcacaggtt ctggcgttat atatccagat atcgcgggcc ttccagtcat
     3001 acaacggata catggtgtaa taaaaccctt caggcgatgc cgtggtccag ggcttatcat
     3061 cggcatatct cagtttgcgc tgagatacca gtcccataaa gcgattgagc gattcatcag
     3121 cacggacacc agtcagtatt gccactccac accggttacc ggcaaaccag gaagaaaatg
     3181 ccggaacaaa ttcttcaaac gtcatggcat accggtaaaa gggaaaatac gtcatgtctg
     3241 taatggcctc ttccggtggc tggcgaaccc aggtcactcc tggctcccag catatccact
     3301 ccggctgaaa ctgcgagacg ccgtttaccg tggtcaggga agtgccaccc agtaaaaggt
     3361 ttccgtcaca tcatggtaca tttcccgcat cttctgaata tgttcaatag tgcaccgata
     3421 ctgggcctcc cagtcaatga acagaacaga gaaatgacgt tttctcctgc gggccacctc
     3481 tgccacaaga tggaaaagca cagtggaatc ctttccacct gaaaaagaca ggcataccga
     3541 cggaaatgtt tcaaaaatcc actcaatgcg ggcgattgcg gcacgcaaaa cattttctcc
     3601 ggtaaggatt ttttcggtta aaagcgtatc actcattttc ctggctttat gtcgaataat
     3661 tctgtttatg cattcagaca aacttcatta ataactcacc gctttgggga taaataataa
     3721 caataaaact cttcagatat taatctggtt gttggatttg ttatcacatc ctttatccac
     3781 ctgcttctta ctggtaacac atctggaaaa tccatgcatt ttcaacatat accatccttt
     3841 caacttgtaa ataaggaaat atgatgcaga tggtgaacaa ttcaaatatc attttgcagg
     3901 aagtattcac tgtaatagaa ttaccccctg ataaagttta ttcactaagg aattattcaa
     3961 cagaaccatt ccctgttagc tttgagcaat agcacttatt tttatatgta tcaggaatac
     4021 gtacgttgat gtaaccaaaa caaagaagcc tgtgtccatg gggttcgtac aattgtgtgt
     4081 ggcacatgac caaatcaggt ttgagacctc ccgctgcaat attttttatt cagactttaa
     4141 cctgtcaggc ctgacagaaa cgctcccaac ttcccttcta tatcactgtc tatagtacgt
     4201 aatcttagca atggcaggcc gcatttcttc agtatactgt ttttaagttc atcacgttca
     4261 gcctgaacac tggtcagatg ataacctcca tccacttcaa taacccctaa tggagtcttc
     4321 ccgaccttat agtaaacaac aaagtcacaa ctggcccggt tacgcatgaa taactgttca
     4381 cggtgtgtaa aatccccccg ctccagcaac accaactgat tcagcgctat ttgtgaatga
     4441 aacatcatac tccgataact atcctgcgac aggatatccc gcaacaggca ggccacaatc
     4501 tgttctgact taaagtgtga atcatttgaa ttcaatctac tattcagtcg ttccagtgat
     4561 ttgtcatact cactgtacag caaatcaaat gctgatatga ccgggctctc gaaaatctct
     4621 ccgtcatcag cgtagtattt gatgtaacga atcagcgccg caatatgtcc agcgtggcgt
     4681 tcaaatacat cattaccagt caccagcgta aatttatttc gggcccttga aacagcgaca
     4741 ttcaccagtt caggattatc aacaaacgcg atgttccgac tgtgctggct gctgcgcttc
     4801 ttatccagca cagtggaaaa cacaatttca tcgcactccc gtccctgaaa tttatgcacc
     4861 gtcgatttca caaaatcagc aggcagcact gtttcagcca gattaacctg tgcattatat
     4921 ggggcgatat atcccctgct actttcctca tcccagtaat gtccttccag agattcaagc
     4981 tccctgagat ttgaaaaatt acgggtatgg tttccttttg ccgtgatgat cagagataat
     5041 gaggcttcac ctgaatcaag cgttagcggt atcagagcat tatcataaaa ctgcttatta
     5101 caaaattgaa tgattttagg atgacagcga taatgctctt taagaagagt tactggcacc
     5161 atattcctga acaacataca aaccgaatca agtagactat atttctcaca attatagtat
     5221 tctgccggtg gagatgggct gttcgggagt aatacaggaa catgtggaag ttgctttcga
     5281 tcccctacaa cgattacatt acgggcacat cccagtccca gaatgcccgg aacaatatcc
     5341 tgctgtgagg cttcatcaat aatcacataa tcaagaagag cccccttacc gatagagtta
     5401 ataatagaat gcgtgctgct accaataacc ggaaaacgct ttataaaacg gttaaattca
     5461 tccctgtatg tctctgccga gaatgaattt cttgttgaaa tatttcttcg aagatgttgt
     5521 tttaaataaa gcacagacca tgatgtcagg cgctccagta aaattttaaa atcagattcc
     5581 tttaatactt cctcatattc attcagagaa acttgttttt cccggatgaa tttttcataa
     5641 taagaaagct gaagagaagt aaatagtgcc agccttctct ccggaatcac caatggtttg
     5701 actttcagta tcctgaagtt taacaacaaa tcaattctgt ttctgaatcc aatccgcctg
     5761 tcagaaaggt aatgaatata agccatcagg tcagttgttt ttcgtgagga aagtcggtat
     5821 ttgtgagaaa aaagctcctc cggtcgaaga ttctgactct gccattgctg caggtattta
     5881 tactcaatat tcagctcatt aatttctgct tttaacgaag cgacctggtt aatggcattc
     5941 aggtgttttt tcacttgttg aagtacatca tcaatcgcat ttgcagacgg tgagtcaggt
     6001 aagacttcct cagaacgcga aatcgacgtt gaaaaaaact gttgcctgtt ctccgtactc
     6061 cccagtcgtg ccaccacata tcccagttgc tgtttgtcca tcttttcata aacattactg
     6121 acagctgagt tattattgga caaaattgcc actgttttat tctgaattaa tatattagca
     6181 acaatattga gtatagtctg tgttttacct gttccaggtg gtccttcaat aactgaaacc
     6241 tgtgaggaaa atgcccgttc gacagccagc aactgacttt cattaagacc aaacggaaaa
     6301 atcaaacccc atggcgaatc acgtttttta ctctgtcctg aacagtatgc atgcaaggcg
     6361 gtatccggat gaggaactat tttctttatc tgcctaagga tattctcatc gatatttctg
     6421 ttattaccgc gggcataaaa aatacgttca ttagccaccc tacacaagta attaaacacg
     6481 gcatgttctg taaatgctga acactgttga aagtcaagac cggtcgtttt catgatgtag
     6541 ggtcgttcac cctcgcgata ataaacagca gcatatttat caccatatat aataaccagt
     6601 tcagcccggg atgttacagc gttccctttc ctttcgagga aaacatttcg caaatttaca
     6661 gttggcgtta tctgacatac agaaagcgga cgagtatatt ttctcccgct gcgaaaatga
     6721 caggtcagca taacctctct gtcgtcattt ccagcccaca gcgtccagtc ggtaatttta
     6781 tgagtcttat tttcaccgtc aacacaaatc gtgaccatat tcttactcag aaattatcat
     6841 ttaacttacg gaaaattgtt aaggcactcc cgatattagc tccggaggat tttccacaac
     6901 tccgggactg ttaacatatt tctgcctgcc atatatacat tgaatttagt cataacagtc
     6961 aataccagaa ttacaggaat gcgatgccga tacgctggtt attgaatact ggtagcccct
     7021 gagcactaat tcatcacgat actctgtatt tactgttttc actgcttttt acaaccattc
     7081 ccccaggtaa aatgctgctc ctccggtgat ggctgtgtca gaaactgcca tcccggaata
     7141 gccacgctga ccgccagcaa tgccgcacag acaccagcca caaacggttt cagaaacgtg
     7201 ggcggggatt tctgaaggga gggtgactgc tccgcctgca ctttcccctt gcgattatca
     7261 aggctcagtg catccagacg caccgcgcca tttgccgcaa gcagcccttc ccggttatcc
     7321 acgcttcccg gcagactcag cgaaaaatcc cctgttcccg tctgaatcag actgccttta
     7381 cgatttgaca ggctttgtgc cgatatctgc agtgtgtcgg catttatcgt accgccatta
     7441 ctgctgaact gcccggcagt ccgggcggaa agcgtatcgg cactcagttg cgcattctgt
     7501 gtagacagat ttccactggt tgcattcagc tcagtatgtt gtccctgtat ccggctgtta
     7561 cctaaatcca gcccctgctc aatgtcccgt aaatccagca gagaaccttc gtgtgccggg
     7621 aatgaggaat acaactgaat atagtcatca ctgtccggtg tcagacgcac atgtcgcaca
     7681 acaccgggaa taataaccag ccggaggata ccgcttttta aatcctgcga aggtgccaga
     7741 acacgggtgg tgacataacc atgatcgacc agacggttct gcaatgtact catcagcaga
     7801 ttaattcctt tcgcccccag gcaatgcccg accgccccgt tggcgatttt ttgtaaaggc
     7861 agccagtgtg gtaacgcatc agcccccttc agttccgtct gtttaatctg aaaacagggc
     7921 gtttcaacag gaaaattaat cttatgggca aaagagccag gtgcagaaag gcgaacatca
     7981 ggggctgaag gcgtcagttg ttgctcaagg gcttcctgac gctgctgctg aatgataaat
     8041 gcttcatctc cgctacgccg gacatccgct gcctgaagag ataaaggcga taataaagca
     8101 gacatcagtg tcagtgacag caaggacgga cagcgccgga ataagctttg ttcctcag
//
LOCUS       DAZFWL020000181         8066 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFWL020000181 DAZFWL020000000
VERSION     DAZFWL020000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.181"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            548..1831
                     /gene="ydfJ"
                     /locus_tag="ACHKNP_RS22275"
                     /old_locus_tag="pgaptmp_004455"
                     /pseudo
     CDS             548..1831
                     /gene="ydfJ"
                     /locus_tag="ACHKNP_RS22275"
                     /old_locus_tag="pgaptmp_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            1920..3380
                     /gene="ydfI"
                     /locus_tag="ACHKNP_RS22280"
                     /old_locus_tag="pgaptmp_004456"
     CDS             1920..3380
                     /gene="ydfI"
                     /locus_tag="ACHKNP_RS22280"
                     /old_locus_tag="pgaptmp_004456"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU4706500.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPAIGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNLIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3416..3619)
                     /gene="ydfZ"
                     /locus_tag="ACHKNP_RS22285"
                     /old_locus_tag="pgaptmp_004457"
     CDS             complement(3416..3619)
                     /gene="ydfZ"
                     /locus_tag="ACHKNP_RS22285"
                     /old_locus_tag="pgaptmp_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HGU4706501.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3797..4483)
                     /gene="rspR"
                     /locus_tag="ACHKNP_RS22290"
                     /old_locus_tag="pgaptmp_004458"
                     /gene_synonym="ydfH"
     CDS             complement(3797..4483)
                     /gene="rspR"
                     /locus_tag="ACHKNP_RS22290"
                     /old_locus_tag="pgaptmp_004458"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HGU4706502.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4572..5318)
                     /gene="ydfG"
                     /locus_tag="ACHKNP_RS22295"
                     /old_locus_tag="pgaptmp_004459"
     CDS             complement(4572..5318)
                     /gene="ydfG"
                     /locus_tag="ACHKNP_RS22295"
                     /old_locus_tag="pgaptmp_004459"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HGU4706503.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5455..7500
                     /gene="dcp"
                     /locus_tag="ACHKNP_RS22300"
                     /old_locus_tag="pgaptmp_004460"
     CDS             5455..7500
                     /gene="dcp"
                     /locus_tag="ACHKNP_RS22300"
                     /old_locus_tag="pgaptmp_004460"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HGU4706504.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHSVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(7545..8063)
                     /gene="ydeJ"
                     /locus_tag="ACHKNP_RS22305"
                     /old_locus_tag="pgaptmp_004461"
     CDS             complement(7545..8063)
                     /gene="ydeJ"
                     /locus_tag="ACHKNP_RS22305"
                     /old_locus_tag="pgaptmp_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HGU4706505.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         2037 a         1843 c         2059 g         2127 t
ORIGIN      
        1 ggtgttctta aagttcgtag ataaacattg tcgtttcagg tatacaggaa tgctaacagg
       61 tggcagcaaa aatcaggcgg tttatggcgc aagctgaagc ggcaactgca aactatctta
      121 tgcagagact ctacacggat tgggtttaaa agtatacata gataacagtt tttatctgaa
      181 aaagaaaaat atcaaggtga tatagcctat atgcctttga tgtggaggaa tgaatgtgat
      241 gggagtgatg tatctgaata gttgaaaaac cgcagacaca ccttatgcaa gaacgtgctg
      301 cggttggctg gtaaattttt cgatagtgcg agtattgaat gatttccagc cgttattgat
      361 tttacgcgta aatccatgaa aaaactactt atctgttggg gagttttttg gggcatatat
      421 gggacagaaa taggccccag atagacactg agatcgaact taggatgctt ttaaaaaaat
      481 gcaactatct gaaaaaacct agaaaacgcc aaggaaacca caggatggga aaaaacacct
      541 gtgaattatg gatttccagt tatattcgct cggcgcagcg ttagtgtttc atgaaatatt
      601 ttttcctgaa tcatcaacgg caatggcgtt aattttggca atgggaacct acggtgcagg
      661 ttatgtggcg cgtattgtcg gagcatttat tttcggcaaa atgggcgaca gaatagggcg
      721 taaaaaagtg ctctttatta ccatcaccat gatggggatc tgtaccacct taattggtgt
      781 gttaccgacc tatgcacaga ttggtgtttt tgcccccatc ttgctggtga cgctgcgtat
      841 tattcagggg ttgggtgcag gtgcggaaat ttccggtgcc ggtacgatgc tggcggaata
      901 tgcgccaaaa ggtaagcgcg gaattatctc ctcatttgta gctatgggaa ctaactgcgg
      961 aaccttgagc gcaacggcaa tctgggcctt tatgttcttc attctcagta aagaggaact
     1021 gcttgcgtgg ggatggcgta taccgttcct ggcgagcgtt gtcgtgatgg tctttgctat
     1081 ctggttgcgt atgaatctga aagaaagccc ggtctttgag aaggttaacg acagcaacca
     1141 acccacagca aaacctgcac ctgctggtag catgttccag agcaaatcct tctggctggc
     1201 aacagggctg cgttttggtc aggcgggtaa ctccgggtta attcagactt tccttgcagg
     1261 ctatttagtg cagacgttat tgtttaacaa agcaattcca acagatgcat tgatgattag
     1321 ttcgattctc ggctttatga ccattccgtt ccttggttgg ttatccgata aaattggtcg
     1381 ccggatcccg tatattatta tgaatacctc cgcgattgtg ctggcatggc caatgctttc
     1441 tatcattgta gataaaagct atgccccgag caccattatg gttgcactga ttgtgattca
     1501 taactgtgcg gtgctgggat tatttgctct ggaaaacatt accatggcag aaatgttcgg
     1561 ctgtaaaaac cgctttaccc ggatggctat ttctaaagaa attggtggtc ttatcgcttc
     1621 cggttttggt cctatcctgg cgggtatttt ctgcaccatg acggaatcct ggtatccgat
     1681 cgccattatg atcatggcat attcagtaat tggtttaatc tctgcgctga aaatgccaga
     1741 ggtgaaagac cgtgatttaa gtgcgctgga agacgctgcg gaagatcaac cgcgtgttgt
     1801 aagagctgcg caaccttcca gaagtctgta aatcctcaat cccttctctt accggagagg
     1861 ggatttttat tcatataaca aaacatatag cttgccatat ttatatttaa ggaattgtta
     1921 tgggaaataa tttgttatca gcaaaagcga cactccctgt ttatgatcgt aataacctgg
     1981 ccccaagaat tattcattta ggctttggtg catttcaccg tgcgcatcag ggcgtgtatg
     2041 ccgatattct tgctacagaa catttcagtg actggggata ttatgaggtc aacttaatcg
     2101 gcggcgaaca gcaaattgcc gatttacaac agcaagataa tctttatacc gttgcggaaa
     2161 tgtcggccga tgcgtggacg gctcgcgtcg ttggcgtcgt taaaaaagcc ttgcacgtac
     2221 agatggatgg cttagaaacc gtgttggctg cgatgtgtga accgcaaatc gcgattgtct
     2281 ctctgacaat caccgaaaaa gggtatttcc actctccggc gatcggacag ttaatgctcg
     2341 atcacccgat ggtcgctgcc gacgtgcaaa atccccacca gccgaaaaca gcaacaggag
     2401 tgattgttga ggcgctggcc cgccgtaaag cggcaggact tcccgcattt accgtcatgt
     2461 catgtgacaa catgccagaa aacggtcatg ttatgcgtga cgttgtcact tcatacgcga
     2521 aagctgttga tgtaaaactg gcacaatgga tcgaagataa cgtgactttc ccatcaacaa
     2581 tggtggaccg tattgtgccc gcagtgacag aggatacgct ggcgaaaatc gaacaactta
     2641 ccggtgtggg cgatcctgcg ggcgttgcct gtgaaccttt ccgccagtgg gtaatagaag
     2701 ataactttgt tgccggacgt ccagaatggg aaaaagcggg agccgaactg gttagtgatg
     2761 tgctgcctta tgaagagatg aagttgcgca tgctcaacgg cagtcattca ttcctggcgt
     2821 atttgggtta tcttgctgga tatcagcaca ttaatgactg catggaagat gaacattatc
     2881 gttatgcggc gtatggcttg atgttgcagg aacaagcgcc gacgctgaaa gtgcagggcg
     2941 ttgatttgca agattacgct aaccgattaa ttgcacgcta tagcaacccg gcgttacgtc
     3001 atcgaacctg gcagattgcg atggatggta gccagaaatt gccacagcgg atgttggatt
     3061 ctgttcgctg gcatctggcg catgacagca agttcgatct gctggcgctg ggcgtcgcgg
     3121 gttggatgcg ttatgtcggt ggtgttgatg aacagggaaa tctgatagaa atcagtgacc
     3181 cactgttacc tgttattcag aaggctgtac aaagtagtgc cgaagggaaa gcgcgcgtcc
     3241 agtcattgct ggcgattaag gcaatctttg gtgatgattt gccagacaat agcttgttta
     3301 ctgcaaaagt gacggaagcg tacttgtctt tattagcgca tggcgcgaaa gcgaccgtgg
     3361 cgaaatattc cgtgaagtaa aaaaagcaat gccgccatcg gcggcattca cacacttagt
     3421 tcatgccgag acgaatcagg tccagaggtg ccagtttctc ttcacaacct tcaacaacta
     3481 cggtttttcc gcggcggatt tgctcagcgg tcagaccttc agtatcaatc gacaggatct
     3541 tgccagtgtg accggtgccg ctaaccataa cacggctgcc agtggtgatt gcgttacggt
     3601 tacgatcgta ggtcgtcata ggtgttttct cctttctgat ttacagtcga agcccacgta
     3661 acgggcgcaa tataaatact cctgtcgagt gaaatatttt ttgtttttga tcaagctcac
     3721 atcttttttg atgcatgcat gagccgtaag gcggggggaa aagcctctca tcccggatgg
     3781 gatgagagta aatgaattac tcttcgctaa accagtcgct gttttcctgg cggatctggc
     3841 gcacggattc gctgatttcc tgcaaatgtt gcgtcattgc acgttctacc gcatcgccat
     3901 cacgtttttg cagggcagag aaaatatcaa gatgctggcg taacagcatt tctggtggag
     3961 aaacatggtc gaaactcata tagcgcacgc gatcaacggt cgctttcagg ttctcaatgg
     4021 tatcccacgc cagttgacag tcggcaatct gcgtcaggag ttgatggaag ttgtcatcaa
     4081 gttcaaaaaa atcatccagt tgcttgcgct caatggcaat gcgttgctgg tgaagatttt
     4141 gttccagttg gtagcactgg ctttcggtaa tcatgctcgc cgcccgacgc gccaccgcgc
     4201 actcaatggc ctgacggata aaactgccgt ttcgcacctg ggccatggaa attttgttga
     4261 cgtagctgcc acgctgcgga cgaatttgaa tcaggccgtt ttccgccagt ttaataaagg
     4321 cttcacgaac cggctggcgt gacacattga aacgaacaga aacttctttt tccgacaacg
     4381 gtgtgcctgg tgcaatcagg caatggacaa tgtcgcgacg aagaatacga taaatctgct
     4441 gattgacagg ctgtgtggga ttaagttgcg tttcgacggt cattcgttct tgcttgtgag
     4501 tgagttaact gcatgagtct accacttttt tgtgacaagc aataacccgg cagttacgcc
     4561 gggtataaaa attactgacg gtggacattc agtccggcat agctttgggt aaccggcatc
     4621 atttccaggg tattgatatt gacgtgagca ggcagcgttg acacccacca gacggcttcg
     4681 ctgacatctt ctggcgtcaa tgcaacggta ttttgatagg ttttttccgc tttaccgtca
     4741 tcgcctttaa agcggacatt ggaaaactcg gtgccaccca ccagacccgg ttcgatgtcg
     4801 gtgacgcgca ccgccgtacc atgcaggtcc gtacgcagat tcaggctaaa ctgacgaaca
     4861 aacgctttcg tcgcaccgta aacgttacca ccggcataag gccagctacc tgccgttgag
     4921 ccaatgttaa taatatgacc atgattacgt tcaaccatac ccggtaagac ggcgcgcgtc
     4981 atatatacca ggcctttgtt gttggtatca atcatcgttt cccagtcttc aacgctggct
     5041 ttatgcgcag gctccatgcc caacgccagg ccggcattat ttaccaggat atcaatattg
     5101 caccactcgg cagggagcga tgccagcatc tcttcaatag cggcgcggtt gcgaacgtcc
     5161 agttgggcga tatacagatt atctcccagt tcgtctttta actcctgcaa ccgctcctgg
     5221 cgacggccag tggcgataac tttatgccct tgttgaataa aacgacgagt aatgcattca
     5281 ccaaaacctg ccgttgctcc agttactaaa acgatcatct cactgttcct caacgctttt
     5341 gtgtgtcaat aacatagcac gtgatgctta agagcgggta atactggttt gcaagattgc
     5401 actccgttat cagtaagcct acgctatgga acacctcgcg ttcaggagaa gaaaatgaca
     5461 acaatgaatc ctttccttgt gcaaagcaca ctgccgtatc tggctcctca ttttgatcaa
     5521 attgccaatc atcactatcg cccggcattc gatgagggaa tgcagcaaaa gcgggcagaa
     5581 attgctgcca tcgcgcttaa cccgcaaacg cctgatttca acaatactat tctggcactg
     5641 gaacaaagcg gagaattact tacccgcgtt accagcgtct tttttgcgat gactgcggcg
     5701 cataccaatg atgaattaca gcgtcttgat gaacagtttt ccgctgaact ggcggaactg
     5761 gctaatgata tctatctgaa cggtgaatta ttcgcgcggg tagatgctgt ctggcagcgc
     5821 cgtgaatccc tggggcttga tagtgaatcc atccgcctgg tggaggtgat tcatcaacgt
     5881 tttgtccttg ccggagccaa acttgcgcaa gctgataaag caaaattaaa agtactgaat
     5941 acagaagctg cgacactgac cagtcagttt aaccagcggt tactggcagc aaataaatcc
     6001 ggcggtctgg ttgtgaacga tatcgcgcag ctggcaggaa tgagtgagca agagattgcg
     6061 ctggcggcag aggcggctcg cgagaaaggt ctggataaca aatggctgat tccgctgctg
     6121 aataccaccc aacaaccggc gcttgccgaa atgcgcgatc gtgcgacgcg tgaaaaactg
     6181 tttattgcgg gctggacgcg agcggaaaaa aatgatggca atgatacccg cgctatcatt
     6241 caacgtctgg tggagatccg tgcacaacag gcgacattac ttggttttcc tcattatgcc
     6301 gcatggaaaa tcgccgatca gatggcaaaa acacctgaag cagcgctcaa ctttatgcgg
     6361 gaaattgttc cagcggcgcg tcaacgtgcg agcgatgaat tagcctccat acaggcggtt
     6421 attgataagc agcagggcgg gtttagcgcg cagccgtggg actgggcatt ttatgccgaa
     6481 caggtacggc gggagaaatt tgatcttgat gaggcgcagc tcaagccata ttttgaatta
     6541 aacacggtgt tgaatgaagg tgtattctgg accgcgaatc agctcttcgg tattaagttt
     6601 gtcgaacgtt ttgatattcc tgtctaccat cctgacgttc gtgtgtggga aatttttgat
     6661 cataatggcg tgggactggc gttattttac ggtgatttct tcgcccgtga ttcaaaaagc
     6721 ggcggtgcat ggatgggcaa ttttgttgag caatcaacgc ttaatgaaac gcattcggta
     6781 atttataacg tttgcaatta tcagaaaccc gctgccggtg agcctgcgtt gttactctgg
     6841 gatgatgtca taaccttatt ccatgaattt ggtcatacgc tgcacggcct ttttgcccgc
     6901 cagcgttatg ccacgctttc cggcaccaac acgccgcgtg attttgtcga atttccgtcg
     6961 caaatcaacg aacactgggc aacgcatccg caggtattcg ctcgctacgc ccggcattat
     7021 cagagcgggg cagcaatgcc tgacgaactg caacagaaaa tgcgtaatgc cagcctgttc
     7081 aacaaagggt atgagatgag cgaactgctt agcgccgcac ttctcgatat gcgctggcat
     7141 tgcctggaag aaaacgaagc aatgcaggat gtcgatgatt ttgaattgcg ggcgctggtg
     7201 gcggaaaata tggatcttcc tgctataccg ccacgctatc gcagcagtta tttcgcccat
     7261 atttttggtg gcggatatgc cgcaggttat tacgcttatc tgtggacaca aatgttggcc
     7321 gatgatggtt atcagtggtt tgttgagcag ggcggattaa cgcgtgaaaa tgggctgcgt
     7381 tttcgcgagg cgatcctttc cagaggtaac agcgaagatc tggaacgcct gtatcgacaa
     7441 tggcgcggta aggctcctca gattatgccg atgctgcaac atcgtggctt gaacatataa
     7501 acaccaccaa tggctgtcct ggagatatcg ccaggccagc ctggttatag cagtaattgc
     7561 agcagctggg cgagggcaaa cctcaccgct aaagctaata ccgtttcgca gtcgccagca
     7621 aaatgcataa ccgcagtata ggtctggcct ttaatatgcc acgcgaacca gacggtaccc
     7681 gctggcgtac catcttcacc gccctccggt ccgccgtagc cggtaatggc aatacttaca
     7741 tcagcatccg cacgctctat ggctccggtt gccatttctg ctgccacttt ctcactcacc
     7801 gcagaatatc gttcaagaga ttgctggctt acgctgagga ttttcatctt tgcctgatcg
     7861 gtgaaagtaa caaagcctgc accgtaaaat ttgggtgtat cttcagctgc acacagggcg
     7921 ctagccaact taccgccggt gcatgattcg gcggtagtta agcgtaattg atccgcgata
     7981 agtctttgac tcaacgcgga tgtcaggttt tcaatagtgt cggtatctgc gagttgtaca
     8041 attttatctt tattaatgtt cactgt
//
LOCUS       DAZFWL020000182         7967 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFWL020000182 DAZFWL020000000
VERSION     DAZFWL020000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.182"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(158..574)
                     /locus_tag="ACHKNP_RS22310"
                     /old_locus_tag="pgaptmp_004462"
     CDS             complement(158..574)
                     /locus_tag="ACHKNP_RS22310"
                     /old_locus_tag="pgaptmp_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HGU4706506.1"
                     /translation="MNKNDLEALSDTRLNEAKCLLDHGFFHGAYYLCGYAVECALKAC
                     IAKSFLQHEFPNKKVVNDSYTHDLSQLLKIANLHQILIADAKNDVSLEINWSVVKDWS
                     EQFRYDNNISKAMAEQLFDAVGDQNSGVLKWVKAHW"
     gene            complement(874..1482)
                     /locus_tag="ACHKNP_RS22315"
                     /old_locus_tag="pgaptmp_004463"
     CDS             complement(874..1482)
                     /locus_tag="ACHKNP_RS22315"
                     /old_locus_tag="pgaptmp_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001415151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HGU4706507.1"
                     /translation="MVTQLAFRDVKFTPINHNNQIWFTSKELAAALKYASTKAVTDIY
                     NKNIDEFTDGMSQVVESTTSGNYRKKTRIFSLRGAHLIAMFARTPVAKEFRRWVLDIL
                     DREIQQSPITKQFTDNELCTLAWLWRASDTMLTACQNVTPLLQVAEHREAGRFTSIEQ
                     EYPRILNKAQAILARETAHVKFRPWQDDKWSRVLTHLRSERL"
     gene            complement(1908..1976)
                     /locus_tag="ACHKNP_RS22320"
                     /old_locus_tag="pgaptmp_004464"
                     /pseudo
     CDS             complement(1908..1976)
                     /locus_tag="ACHKNP_RS22320"
                     /old_locus_tag="pgaptmp_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2235..2582
                     /locus_tag="ACHKNP_RS22325"
                     /old_locus_tag="pgaptmp_004465"
     CDS             2235..2582
                     /locus_tag="ACHKNP_RS22325"
                     /old_locus_tag="pgaptmp_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706508.1"
                     /translation="MTIKATDIEILHRYAEGVMERSNHHAKNVGAAALTLLGGVIWKA
                     LPGSIEIRTYNGSLANMVWWQSERTLKNYAISYNHNSCEIEMRDESVKGAVLFSISNE
                     TTPEKILSQLSEL"
     gene            complement(2787..3488)
                     /locus_tag="ACHKNP_RS22330"
                     /old_locus_tag="pgaptmp_004466"
     CDS             complement(2787..3488)
                     /locus_tag="ACHKNP_RS22330"
                     /old_locus_tag="pgaptmp_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4706509.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(3485..4504)
                     /locus_tag="ACHKNP_RS22335"
                     /old_locus_tag="pgaptmp_004467"
     CDS             complement(3485..4504)
                     /locus_tag="ACHKNP_RS22335"
                     /old_locus_tag="pgaptmp_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU4706510.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGTYPNQVNTKDKLTKDNIKPYSSENSGESSDLPENDLPVVK
                     ADAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTVGKPKLDLTNTDWI
                     YGVDL"
     gene            complement(4501..5040)
                     /locus_tag="ACHKNP_RS22340"
                     /old_locus_tag="pgaptmp_004468"
     CDS             complement(4501..5040)
                     /locus_tag="ACHKNP_RS22340"
                     /old_locus_tag="pgaptmp_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HGU4706511.1"
                     /translation="MQPLPYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRTRQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPELAGPLMAMVTTMLGAI"
     gene            complement(5110..5340)
                     /locus_tag="ACHKNP_RS22345"
                     /old_locus_tag="pgaptmp_004469"
     CDS             complement(5110..5340)
                     /locus_tag="ACHKNP_RS22345"
                     /old_locus_tag="pgaptmp_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4706512.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            5445..6134
                     /locus_tag="ACHKNP_RS22350"
                     /old_locus_tag="pgaptmp_004470"
     CDS             5445..6134
                     /locus_tag="ACHKNP_RS22350"
                     /old_locus_tag="pgaptmp_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU4706513.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            6216..6479
                     /locus_tag="ACHKNP_RS22355"
                     /old_locus_tag="pgaptmp_004471"
     CDS             6216..6479
                     /locus_tag="ACHKNP_RS22355"
                     /old_locus_tag="pgaptmp_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706514.1"
                     /translation="MSNKTLVKKSNNCVDAYADAFGYSSFGDGSQRLGSISFFHNVTE
                     WPVDESDDVENIKYNIATIRMPEELMLKLADFIRDQHDKAKTN"
     gene            6492..6935
                     /locus_tag="ACHKNP_RS22360"
                     /old_locus_tag="pgaptmp_004472"
     CDS             6492..6935
                     /locus_tag="ACHKNP_RS22360"
                     /old_locus_tag="pgaptmp_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706515.1"
                     /translation="MKHDYYEAPNLNDKIAKAAESFCLHSSASSQLAGISGKRWDMLQ
                     SSGSSGSGSDNMPEKVAKLESDVAHIKRDVDELKTDVKAIDKNMITILARLDSIKESL
                     TNKPSSDAVDRKISDAKLAVLLGVPAIIAAGTGLYKLSMYFFFSA"
     gene            7486..7692
                     /locus_tag="ACHKNP_RS22365"
                     /old_locus_tag="pgaptmp_004473"
     CDS             7486..7692
                     /locus_tag="ACHKNP_RS22365"
                     /old_locus_tag="pgaptmp_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HGU4706516.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKRKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNALNH"
     gene            7768..>7967
                     /locus_tag="ACHKNP_RS22370"
                     /old_locus_tag="pgaptmp_004474"
     CDS             7768..>7967
                     /locus_tag="ACHKNP_RS22370"
                     /old_locus_tag="pgaptmp_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4706517.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAF"
BASE COUNT         2149 a         1864 c         1735 g         2219 t
ORIGIN      
        1 ccacgttctg agaagtcact gataactaaa atatatctcc aagatagatc tgggtaataa
       61 aaccacattg cagcatcaat agttaaattc tgtaacttga gtttttttaa caaaaattga
      121 cctgagaatt ccatatcttt tgtcaactct ctgccgatta ccagtgtgct tttacccatt
      181 tcaaaactcc agaattttgg tcacctacag catcaaacaa ttgttcggcc atagctttac
      241 ttatgttatt gtcgtatcta aattgctcgc tccagtcttt aacgaccgac cagttaatct
      301 ccaacgaaac atcatttttt gcgtcagcaa tcaaaatttg atgtaagttg gcaattttga
      361 gaagttgcga caaatcatga gtatatgaat catttacgac ttttttgttt ggaaactcat
      421 gttgtaaaaa tgacttggca atacaggcct tcaatgcaca ttcaactgca tacccacaaa
      481 gataatatgc accatgaaaa aagccatgat caagcaagca tttggcctca ttaagcctag
      541 tgtcagataa tgcttcaagg tcgtttttat tcatacaatt ataacttgaa ttatcctaat
      601 ttttatgatt atacccttat caggtatgat gtaggaactg aaaacaatat tggatatcat
      661 tttcggtatt tctctgcaaa ttccagccca caggatctcg gtgtacaatc tgtcgctgat
      721 cgcgcaaatt tagggtcaat cacttcattt tttgcatcaa accctcacta acatcgaacg
      781 attatagtac aagtagtgtt cttgacttct cattaaaccg tcagcattca attaattaca
      841 aaatactagc ggcatttctc tcccgcaact ttattacagc cgttcagaac gtaaatgcgt
      901 caatactcga ctccacttat catcctgcca cggccggaat tttacatgtg ccgtttctct
      961 ggcaaggatt gcctgtgcct tattgagtat ccggggatat tcttgttcga ttgaagtgaa
     1021 gcgaccggct tcgcggtgtt ctgctacctg aagaagcgga gtgacgtttt ggcaagcggt
     1081 taacattgtg tcacttgccc gccataacca agcaagtgtg caaagttcgt tatcagtgaa
     1141 ttgttttgtg attggggatt gttgaatttc tcgatcgaga atatccagca cccagcggcg
     1201 gaattctttg gccacaggag tgcgggcgaa catggcgatc agatgggcgc cacggaggga
     1261 gaaaatgcga gtcttcttac gataatttcc tgaggtcgtt gattcgacga cctgagacat
     1321 tccgtcagta aactcatcaa tatttttgtt gtagatgtcg gttactgctt tggtgctggc
     1381 atattttaat gctgccgcca attctttgct ggtaaaccag atctggttgt tgtgatttat
     1441 aggagtgaat tttacatcgc ggaatgcgag ctgagtgacc atagcgataa tctcgttttg
     1501 aatttgagct atcaccaccg acgacgccaa tcgactggtg gtggactgtg caaggttggc
     1561 gtaaccggtc aaaacgaaat ccggcgcttc cgaagaagcc ctcacacagc ccaccataat
     1621 ttttgtgtag acgtgcactg cgcataaaaa aaccgcgaac gcggttatgc gtcgttttga
     1681 tgtccgggac gccaatcccg tgtgccgatt ttgcggcaac gcacagaata tagcgccgga
     1741 taaataatgt cgtcaaccct tgcatatggg gggcgcgatg atcactacta ccaccataat
     1801 cgctccaact gttgcaacta ctttaggcta agacatcacg atttccatat cacctttagc
     1861 cgccagacaa acagagactc cctgttcttg gctgccaaaa acaccgaatg caaaaaatcg
     1921 caggtgtatt tctgcatctg tggagggaga agagagtttg gattgtatgt gcgcataaac
     1981 gtccccgttt agcgcagaag tcaccgtagt tgttcatatt ctgatgacat gataatttcc
     2041 tattgaatag aaaaaattaa cacgactcat tcttatgact ggaaaaagca atcatgctaa
     2101 taatcattgt tgttccagtt ccaaatgcag cgaaataatc accaatattt tttctaccgg
     2161 tttcgacttt gatgctatat tctgctaaca cctaactaac actacgtttc acacagcaat
     2221 tacatggaaa taacatgact atcaaagcga ctgacataga gattcttcat cgttatgctg
     2281 aaggggtaat ggaacgttct aaccatcatg caaaaaatgt tggtgcagcc gctcttacat
     2341 tgctaggagg tgttatctgg aaagccctgc ccggatcaat cgagataaga acgtataacg
     2401 gaagtcttgc aaatatggtg tggtggcaaa gcgaaagaac attaaaaaat tatgcaatct
     2461 catacaacca taattcatgt gaaattgaaa tgagagatga atcagtcaaa ggtgctgtat
     2521 tatttagcat ttccaacgaa accactccag aaaaaatatt atcacagctt tctgagcttt
     2581 aattaaaggc ggtgctaaca ccgcccatac cattactcca catcttacgc aacttgataa
     2641 ttatgctcta ttaaataccc acaatatttc atcaatgcat gggtcagcta caaaatgttt
     2701 accgtggtaa ttcctgtttt cgcaacatct caaaagaaca ataaacactg tatttcttca
     2761 agtaacgaag aatctttctt tgcccgtcat acacttgctc ctttcagccc aaacttagct
     2821 ttgatttctg cgatcttcgc cagagcctgt gcacgattta gaggtctacc gcccataaca
     2881 ggaagttgtt ttactggttc aggtatcgtc tcaccacggt taattcgcgc tgtcatacag
     2941 gtcagttcat cggcagcctt gcgccgtaat tccgcgtcag tcagcgcatt ggcccgcatg
     3001 ttctggtaca ggttggtaac caaccagtaa tgcgcgttcg atttccacgg ataagactct
     3061 gcatccggat acaggccacg cttccggcaa tactcgtaaa ccatatcaac cagctcgctg
     3121 acgtttggca gcccggcgtt aacggatgct tcttcccggc accatgcaac aaactgcccg
     3181 ggtgatggca gaaatggtct attctgccga cgggcaacac gcattccggc gctcacctgt
     3241 tccatcgtgg tgatcccgtt ttcccggaaa gccagaaccc actggcggcg gatttcgttc
     3301 agttcgttct ggtcacgatt agccaggctc gccgggaaag ttgccagcaa ctggctgaat
     3361 acaccgttga ttatctgcgc tacctgctgt acctgcggct tttcgtcgta ctgttccggc
     3421 atgttgttgg cgatccgacg catctgctca cggtcaaagt taatcatctg tgcggcgatg
     3481 tttttcatag atccaccccg taaatccagt ctgtgtttgt caggtcgagt tttggtttgc
     3541 cgactgtcac gccagcctgt tgcttgttcc ggttgatttc gagctgggtc cacttgtcgc
     3601 ggagtttggc cggactcagc acgttaccgg accagaagtt gtcctggcag gcccagcgga
     3661 acagtacaca catgtcgcgg tggttacgtc catcacgttc acgcatcaga cggatatcgt
     3721 tagcccaccc tgcaaaattc ggttttctgg ctgatggcgc gatggtcttc accatgtcaa
     3781 acatccactc tgcggcggtc aggtcttctg ctgtccccca cttgctgccg ctctgaatcg
     3841 cagcatccgc tttcaccaca ggaaggtcgt tttctggcag gtcagaggat tcgccagaat
     3901 tctcggacga ataaggtttt atattgtctt ttgttagttt gtcttttgtg tttacctgat
     3961 tcgggtaagt gcctttacct gatttgggta aacttttctt acctgattca ggtaaattta
     4021 cctctttcag gtaaacttta tttttcttac ctgattcggg taatgttgac cattcactga
     4081 ccacattatt aatgccgata ttccgcccgc tctgaataaa aatcccacgc tttaccagaa
     4141 cactttttgc agcagaacac ttgtgcggca atatcccggt caactcggaa agttgctcgt
     4201 tgctcaccca gtccagtttt ttattaaagc catatgtttt gcgcatgaca gccaggaaga
     4261 ccagaagctg gtgctgtgtt aatccggcca gcattacagc ttccagcaac tcatttgcaa
     4321 tgcgcgtata accatcatcg agatctgcca cgcgcggctc cttttgtgcc gcatccggca
     4381 ctggaaaatt gaatatctca gcagtgtttg ccataattcc tcccgcaatg agtgtgttac
     4441 gatttgcacc tgaaagtcgg ttctgttcca gcagaccggc tttcgccatt tctgaacctg
     4501 tcatatcgcc cccagcatgg tagtaaccat cgccatcaat ggaccagcca gctctgggtc
     4561 cacacgaaac atcgacacaa taccttcact aatttccttc agtttctggt ggcgtggtgc
     4621 gttgagaatg acagcctgtt ttgcctcact gagttccttt tccatttcag ccaacctagc
     4681 catgaagcta tcctgctcaa ccaggtaacc gcgatattcc agcggtagta ccgccagaat
     4741 tgccggggtc agttcacgca cgttatttcg gtatttttca gaatcgaatt tgttatcgag
     4801 gaagcggaac agcttctggc gtgtacggct gacatcatca gggaaatcga tggtgccgcc
     4861 gccctgctcc cgatactcat tcacaatgag tgtggcaacg acatcctgat tatctacagc
     4921 cgaccaggcg cggacggcat cacggatttt ttcgtggcct ggcacctgtt ttgtttgaga
     4981 acgatttatc accgcagtcg ggctaaatcc gctagtctgt tggtatggaa gtggttgcat
     5041 aattgactcc tttagtttga attgactgtt aagttgattg cttattgtta aagagcgtga
     5101 aatggaaatt taagctgcgt tcttttcggt gtgtggaaac aacttcggaa gatccgggcg
     5161 aatctggtat gccttcacta ctccaccagt agccgtaaca atgctgccga catgttcagg
     5221 ggataccttt gctttgttgt gaagccactt atagacggcc tgctgtgaaa cttcgcaggc
     5281 agcgcccagt ttcttttgtg aaccaacgat attgatcgct gttttgatag ctgggttcat
     5341 aacaacctcc gtggttaatt tgaatcaaga ttaaaactat ggttgttttt agtcaacaac
     5401 cattttcgtt tgatggaata aaaccttggt tgtacatttg gactatgaaa acaacactct
     5461 cagaaagact taaagaagcc agattagcgc gaggccttac acaaaaggcg cttggggatt
     5521 tggtcggggt tagccaggct gctattcaga aaatcgaaac agggaaagct aatcaaacaa
     5581 ctaaaatcgt ggagatcgcg aacgctttgg gtgtgcgcgc agaatggtta tcttctggcg
     5641 ttggaaatat gtcagacagt acagtgcaac caatacaatc aactgtcagc cattccaaat
     5701 acttcaagat tgacgttctt gatatagaag tcagtgctgg gccgggagtc atcaaccgtg
     5761 agtttgtaga agttctacgc tcggttgagt actcgtttga cgatgctcgt cacatgttcg
     5821 atggtaggaa ggcagaaaat atccgcatca ttaacgtgcg tggtgacagc atgtcaggaa
     5881 cgatcgaacc aggtgatctg ctgttcgttg atatcacggt taaatctttc gacggtgatg
     5941 gtatctatgc atttctgtac gacgacaccg cccatgtaaa gcgcctgcaa atgatgaagg
     6001 ataaactgct ggttatctct gataacaaga gctactcacc gtgggaccct atcgagaaag
     6061 acgagatgaa ccgggtattc atattcggta aggttattgg gagcatgccg cagacgtaca
     6121 gaaaacacgg ataatcagcc gcgtgttgat gaggcttttg ggtaatacgc tgaagaaaat
     6181 accttataaa aaataacatt acgggaaagg caaaaatgag taataaaaca cttgttaaaa
     6241 aatcaaataa ctgtgtcgat gcatacgccg acgcgtttgg ctactcttcg tttggtgacg
     6301 gctctcagcg cctaggttca atttcttttt ttcataatgt aactgaatgg cctgttgatg
     6361 aaagtgacga tgttgagaac ataaagtaca acattgcaac cattagaatg cccgaagaat
     6421 tgatgcttaa attggctgac ttcattcgcg accagcatga caaagctaaa acaaactaat
     6481 cgtaattaat tatgaaacac gattattacg aagctccaaa cctaaatgat aaaattgcaa
     6541 aggcggcaga atcattttgt ttgcactcca gtgcgtcttc ccagctagca ggtatatctg
     6601 gaaaaagatg ggacatgcta caatcaagtg gtagcagtgg gtctgggagt gataacatgc
     6661 ctgaaaaagt tgcaaaactg gagtccgatg ttgctcatat caaacgagac gttgatgaac
     6721 ttaaaacaga cgttaaagct atcgacaaaa atatgataac tattcttgca cgtctggact
     6781 ctataaaaga atccttaaca aacaagccat caagtgatgc tgtcgataga aagatttcag
     6841 acgcaaagct tgcggtattg cttggtgttc cagcaatcat cgctgcagga acaggacttt
     6901 ataagctatc aatgtacttt ttctttagtg cttaagaaaa tgctcgcacc cttgcattag
     6961 gtaaagcgat ttttcattcc ccattaccct cgctcttaac caatcatcta aacccggcca
     7021 ccgtgccggg ttttcttttg ccctcccctc atcatacacc gttcaaaaaa accaccacga
     7081 cctcgcttca gttatcgcta tgcgatgcaa gtcacaaaat aaatccatct taaatacaac
     7141 cagttatatc taaaacaacc aacaaaacaa cttttgttgt tgacgataaa acaactatag
     7201 ttttaaatga gttcatcgca acaacacaac gatacggcaa tcacctgatt caccgttgcg
     7261 atgaccgctt agatccgcag cttgaatttc agcaggcttc ggggagtgcg agggatgaaa
     7321 cggacgcgtg aacgtcggtg tgaccagctg aaatcaactc aacatttcat accttagtcg
     7381 cttcaacgag gcggcttagt tatgacaacc ggcggccatc caccgcctga atacgcgcag
     7441 aagtctttat atgttcagca gcccagctta cgggcaggag tttttatggt tcatcaacat
     7501 tacggaacgc agaccgttaa tcgcggtgcg gtcatgccag gaatgctggt caaacgcaaa
     7561 gatggtacct ggactgcatc agctaattta cgcggacgac tttatctgca tcgcggcatt
     7621 gagcgcactt atacccgtga cttgctcgtg gaagtttttc tcgacggacg cggcaacgct
     7681 ctgaatcact aatccccttt cctgttttcc gaatcagcct ggcattccgc gggcgatttt
     7741 ttcacagcca ttttcaggag ttcagccatg aacgcttatt acattcagga tcgtcttgag
     7801 gctcagagct gggcgcgtca ctaccagcag atcgcccgtg aagagaaaga ggcagaactg
     7861 gcagacgaca tggaaaaagg cctgccccag cacctgtttg aatcgctatg catcgatcat
     7921 ttgcaacgcc acggggccag caaaaaagcc attacccgtg cgtttga
//
LOCUS       DAZFWL020000183         7820 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFWL020000183 DAZFWL020000000
VERSION     DAZFWL020000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.183"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            8..532
                     /locus_tag="ACHKNP_RS22375"
                     /old_locus_tag="pgaptmp_004475"
     CDS             8..532
                     /locus_tag="ACHKNP_RS22375"
                     /old_locus_tag="pgaptmp_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HGU4706518.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            616..1302
                     /gene="lpfB"
                     /locus_tag="ACHKNP_RS22380"
                     /old_locus_tag="pgaptmp_004476"
     CDS             616..1302
                     /gene="lpfB"
                     /locus_tag="ACHKNP_RS22380"
                     /old_locus_tag="pgaptmp_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HGU4706519.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1326..3857
                     /gene="lpfC"
                     /locus_tag="ACHKNP_RS22385"
                     /old_locus_tag="pgaptmp_004477"
     CDS             1326..3857
                     /gene="lpfC"
                     /locus_tag="ACHKNP_RS22385"
                     /old_locus_tag="pgaptmp_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HGU4706520.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3868..4923
                     /gene="lpfD"
                     /locus_tag="ACHKNP_RS22390"
                     /old_locus_tag="pgaptmp_004478"
     CDS             3868..4923
                     /gene="lpfD"
                     /locus_tag="ACHKNP_RS22390"
                     /old_locus_tag="pgaptmp_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HGU4706521.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4929..5453
                     /gene="lpfE"
                     /locus_tag="ACHKNP_RS22395"
                     /old_locus_tag="pgaptmp_004479"
     CDS             4929..5453
                     /gene="lpfE"
                     /locus_tag="ACHKNP_RS22395"
                     /old_locus_tag="pgaptmp_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HGU4706522.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5706..7397
                     /gene="eptB"
                     /locus_tag="ACHKNP_RS22400"
                     /old_locus_tag="pgaptmp_004480"
     CDS             5706..7397
                     /gene="eptB"
                     /locus_tag="ACHKNP_RS22400"
                     /old_locus_tag="pgaptmp_004480"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HGU4706523.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7489..7565
                     /locus_tag="ACHKNP_RS22405"
                     /old_locus_tag="pgaptmp_004481"
     tRNA            7489..7565
                     /locus_tag="ACHKNP_RS22405"
                     /old_locus_tag="pgaptmp_004481"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7523..7525,aa:Pro,seq:cgg)
     gene            7600..7733
                     /locus_tag="ACHKNP_RS22410"
                     /old_locus_tag="pgaptmp_004482"
     ncRNA           7600..7733
                     /ncRNA_class="other"
                     /locus_tag="ACHKNP_RS22410"
                     /old_locus_tag="pgaptmp_004482"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         2031 a         1803 c         1986 g         2000 t
ORIGIN      
        1 gttataaatg aaaaaggttc tgtttgcctt atctgcgctt gcgctgacct ctacttccgt
       61 attcgcagct gatgcgggcg acggtactgt taaattcacc ggtgagattg ttgacgccgc
      121 ttgtgttgtg tctccagaca ctcaaaaaca agaagttgtt ctgggtcagg tgaacaagtc
      181 tgtatttact gctatgggtg ataaatccac agcaacgccg ttcaaaatca aactggaaaa
      241 ctgcgatatc tccactttca aaaatgtgga aatcagcttt aacggcgttg gcgatgcaga
      301 caacagcaaa ctggtttctg taagcactga accaggtgcc gcaactggcg taggtatcgg
      361 tatttatgat aacaccaata ccctggttga cctgaatact ggtaaatctg ctactgtcct
      421 gaaagaaggt cagaccgttc tgtatttcac cgctaactat gtctctacca aagatgcagt
      481 aaccacgggt tacggtaacg ctgaagtcga cttcaacctg acttacaact aatcgatcct
      541 gacggaacga tcatctctca atggcaacag agactgtgtt gccattattc acaccaatga
      601 ctcagggacc gaatcatgaa acgtattagc tttgctgtgc tggcactttt aatgaccgca
      661 caacagtgtt ttgctggtgg cgtagcctta agcacgacgc gtgttattta tgatggcggc
      721 aagaaagaag cgtcgctgac cgttgaaaac cataataaaa atgaagagtt tttgatccag
      781 tcatggattg atgatgtgaa tggcaataaa aaaacaccgt ttattattac accgccatta
      841 ttcaaacttg atcctgataa aaataacgtc ttacgtatcg tcaatattga agatacctta
      901 cctaaagatc gtgaatccgt ttactggatt aatgtaaagg ccattcctgc gaaaagtgct
      961 gaaaatgaaa ataaaaacgt cttacagata gccgttcgca cccgtttgaa gttgttctat
     1021 cgtccaaaag gtttgccggg aagcaacctc gaggccatga aacagttacg tttccagcgt
     1081 caaaataacc aaattgtggt taataacgac tccgcttata acctgacctt taatcagttt
     1141 tccattaatg gacaaaagat tgaacgggcg ggaatggttc tcgcgaaagg aaagttaaac
     1201 attaaccttc cagccggtac aggcaacgcg cacgaagtga aatacagcat tattaatgat
     1261 ttcggtgttg ctggcgaaat gctgacgaag ccgattaact gatcctttta caaggcgctt
     1321 ttactatgca ttggatgcat ttacctctct atcactacag agcgcatttt tccttctctc
     1381 tgctggcatt aacgattgcc agctcgttac ctgcgtatgg cggaaaattt aaccctaaat
     1441 ttctcgaaaa cgttcagggt atcgatcaac acatcgactt gtcggtatac gactcacctg
     1501 tcggccagca aatccccggt aaataccggg tttcggtgtt tgtaaacgaa gaaaaaatgg
     1561 cttcccggac gctggatttt agtaccgcca gcgaagcgaa acgcaaagcc agcggtgagt
     1621 cgcttatgcc ttgccttagc cgggtgcaac tggaagaaat gggcgtgcgc gttgacagct
     1681 ttccggcgct gaagatgtca ccaccagaag cctgcgtagc gtttgatgaa atcattcccc
     1741 aggccaccag ccgctttgat tttaatactc agacgctgca tctttccttt ccacaggcgg
     1801 caatgatgat gaccgctcgc ggcacggttg atccttctcg ctgggacgaa ggaatacctg
     1861 cgctgttgct ggattacagc tttagtggca gcaatggacg caatgaaggt acaggttcat
     1921 cttcagattc gaccagtgac agttattacc tgaacctgcg cagcggtttg aacgttggtc
     1981 cgtggcgttt acgtaataac agtatctgga accgtaccga tggcaaaaac caatgggata
     2041 acgttggtac atcgttaaac cgggcaatta ttccacttaa atcacagata actctgggtg
     2101 ataccgccac gcctggcgag atttttgata gcgtacaaat gcgtggggcg ttactggcat
     2161 ccgacgatga aatgttgccg gacagccagc gtggctttgc tccggtagtg cgcggcatcg
     2221 ccaaaagcaa cgctgaagtg agtatcgaac agaacggcta tgtcatttat cgcacttttg
     2281 ttcagccggg tgcatttgag attaacgatc tctacgcaac ctccggcagc ggcgacctga
     2341 cggtgatcat taaagaagcc gacggaagtg aacaacgttt tatccagccg ttttcggcgg
     2401 tggcgatttt ccagcgtgaa gggtatctga aatacagtct ggctgccggt gaatatcgtg
     2461 ctggtaatta cgacagcgat aaaccacatt tcgggcagtt taccgccatg tacggcttgc
     2521 cgtggggtat gaccgcctac ggtggtgcat tgttgtcggc agattacaac gctttagctc
     2581 tgggcctggg gaaaaacttc ggtaccattg gcgcagtatc ggtggatgtc actcaggcga
     2641 aaagtcagtt acgcaacaat gaaaaagagg aaggccagtc ttatcgcttc ctctattcga
     2701 aaagttttga gggtggcact gacctgcgtc tgttgggcta caaatactca accagcggct
     2761 actacacctt ccaggaagca actgatgttc gcagtgacgc cgacagtgat taccgccgtt
     2821 atcacaagcg cagccagata cagggcaata ttacccagca actgggagac tacggctcgg
     2881 tttatttcaa tatgacgcag caggattact ggaacgttga cggtaaggaa aactccttaa
     2941 gcgccggtta tcacggtcac attggtcggg tgaattacag cgtggcctat acctggacga
     3001 gaagtccgga atgggaagaa gacgatcgct tgtggtcatt cagtgtttcc atccctctgg
     3061 ggggcgcgtg gagcagttat cgcatgacta ccgaccagaa tggcaagacg tcgcaacagg
     3121 cctctgttag cggcacgtta ctggaagatc gtaacctgag ctataacgtt cagcagggtt
     3181 acaccagtaa tggcgtaggc tacagtggca gcgtcaacat ggggtatatg ggtggctctg
     3241 gtaacattga cgtaggctat aactattcta aagataacca acaggttaac tatggcgtgc
     3301 gcggcggtgt catcgttcac agtgaaggta taacgctttc gcagccttta ggcgaaagtc
     3361 tggcgattgt ttctgcaccg ggcgcgcgtg gtggacatgt ggttaacagc agcggcgtag
     3421 aagttgactg gatgggcaac gccgtggtgc cttatctcac accatatcgt gaaaccattg
     3481 tggaattgcg tagcgacacg ttggggcaaa acgttgaact acaggaagcg ttccagaaag
     3541 tcgtgcctac tcgtggtgca gttgtgcgtt cccgctttga tacccgcgtc ggttaccgtg
     3601 tgctgatgag cttaaaacag gcgaatggca acgcagtacc gtttggtgca accgcagcgt
     3661 taattgatga gagcaaacct gccagcagca ttgtgggtga agaaggacag ctttatatca
     3721 gcgggatgcc agaagagggc gaattgcagg tgagctgggg gaacgagcag gcgcaacgtt
     3781 gccgtgtgcc attccgcttg ccagagaaca aggacaatgc ggctatcgtg atggttaatg
     3841 cggtatgcga gaaataagga tatttagatg aaaattaaaa atttgttggc aggtatggtg
     3901 ctgttaggga cgagcacttt tgctggaaat atatgggctg cggattgggg accatgtcag
     3961 acatcaaatg gggtggccca tgagtatagt tttgatttcg tacagactat ccaggtacct
     4021 tcagaaaata aggctgggaa aatattaact cagccctttg ctttaggtac aaaatattcc
     4081 gcttattgtg aatgcccgga tcccattcca gataatggag ttgtgactta ttttaaaggt
     4141 gtaacgcttc tgcctgagcc tggaactgta gatgggtact ataaattcaa taattacatt
     4201 gatattctca ccaaaatata tgtctacaca caattagata tcccggtgcc ttttaccgat
     4261 cgaagcaatg gcacggcaca gaaagaatgt acgccctata ctgctaacaa ttgggggaca
     4321 ggtggtaaag gaagtatcag tatttatatt tcacatcctt ttgttgggca aatgattatt
     4381 cctaaaacac gagttgcgtc cctctttggg acaaaaaaga aaggtgttta caatgatagt
     4441 cagcccatgg ccaatgtcag tatttccggg agtattacag ttactcaagg ctgtgaactt
     4501 gccgctggca ccgtactgga tattccattc ggagaatatc aggcccatga ttttaaaggg
     4561 cgtgccggac aaccaccaca aaatgttcag aaggtacaaa aagaattaag ctttgactgc
     4621 aataatattt cagatggcgt caaaatttat ctcagtattg atgcaacacc aaatactgcc
     4681 tatcccagcg caattgattt aggcaacgcc gatgttggtg cagtcattga ggacggtaaa
     4741 ggtaatattc ttaagccgaa tgacagtaat agcttattag aaatgaaccc aggctcgctg
     4801 tatgaagatg tcaaacgcaa agcgacaacc acaattaccg cttatcccgt tagcactacc
     4861 ggaaaattac ccgctgccgg ggattatagc ggtattgcta ccatacatgt cgaactggag
     4921 taacagtcat gaagcgaaca atacttgcgt tatgcctgct gcctctttcc ttttcgtcat
     4981 tcgcggcaac cacggattta gggccgaaag gaacattgaa attcaccctt aaaatcagcc
     5041 agggtgcttg cgaactggtg aaagacagcg ttgaggttga catgggcacc gcggttctca
     5101 aaaagccggt aagaatcgga acggaaatta atcccactcc tttttccatc ggcctgaaaa
     5161 actgtagcga agtggtcaga gcctacgtga ctatggacgg tacacccaat gcagacgact
     5221 caaatctttt tgcgctggat gcgggtggcg ctacaggcgt tgggctgaaa atcaaaaccg
     5281 ctgctggagt acaacagttc ccggaaaata caacgccgac accggttgag tttgcaatta
     5341 acggtgatgg cgcacatcaa ctgaactata tcgccagcta cgtgcctatt cgcgccgatg
     5401 caactattgg tcgcgcagat gcaacggtcg actttactgt acagtacgaa taatttcaca
     5461 tcttaagggc cagttaactg gcccttttta ctgctttttg taacgctaaa gtctcttttc
     5521 aaacttgcat ttttgtaaat ttgtgcttca tgcacactct ttccccacac tttttcgctt
     5581 tgctgtggtc tacttattcg cgcgtgtaga ttttacttat ctgactacct ccgcactttt
     5641 cccctgccgg gcctgaaaag ccgctaagca gggtgttatc acctgtttgt ccagggtttg
     5701 tttgcatgag atacatcaaa tcgattacac agcagaagct gagctttttg cttgcaatct
     5761 atattggcct ttttatgaat ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg
     5821 attttaccgt ctggaaaggc gtttctgctg ttgttgaact ggccgccacc gtactggtga
     5881 ccttcttttt actacgtctt ctatcgctgt ttggccgccg cagctggcgt attctggcgt
     5941 cgctggtggt gctcttttcc gcaggtgcca gctattacat gaccttcctt aatgtggtca
     6001 ttggttatgg catcatcgct tccgtcatga ccaccgatat cgacctgtca aaagaagttg
     6061 ttggtctgaa ctttattctc tggttaatcg ccgttagtgc attgcctctt atccttatct
     6121 ggaataaccg ctgtcgctac accttgctcc gacaactgcg aaccccaggg cagcgtattc
     6181 gcagcctggc ggtcgtcgta ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg
     6241 atatccagca gaagaaagtg gagagggcga ccggcgttga tttgccgagt tatggcggtg
     6301 tcgtagcgaa ctcttatctg ccatcaaact ggctttctgc gttggggctg tatgcctggg
     6361 cgcgggtcga tgaatcttcc gataataatt cattgcttaa tccggcgaag aaattcactt
     6421 atcaggcacc gcaaaacgtt gatgacactt atgtcgtgtt tatcatcggt gaaaccacgc
     6481 gttgggacca tatgggcatt ttcggctatg agcgtaatac cacgccgaaa ctggcccagg
     6541 agaaaaatct ggcggcgttc cgtggttact cctgtgatac cgcaaccaaa ctctcactgc
     6601 gttgcatgtt tgtacgtcag gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac
     6661 agaacatttt cgcggttctg aagcagttag gattcagttc tgacctctac gctatgcaga
     6721 gcgaaatgtg gttctacagc aacacgatgg cggacaacat tgcttatcgt gagcagattg
     6781 gtgcggagcc acgtaatcgt ggcaagccgg tagatgatat gttgctggta gacgaaatgc
     6841 agcaatcgct agggcgcaac ccggatggta agcatctgat cattctgcat accaaaggtt
     6901 cgcattttaa ctacacccag cgttatccgc gtagcttcgc gcagtggaag ccggaatgta
     6961 ttggtgttga tagcggctgt accaaagcgc agatgatcaa ctcctatgac aactcggtga
     7021 cctatgtgga tcactttatc tccagcgtga ttgatcaggt tcgcgataag aaagcgattg
     7081 tgttctacgc agctgaccac ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc
     7141 cgcgtgaact ggcaccgccg gagcagttcc gcgtaccgat gatggtctgg atgtcagata
     7201 aatatctgga aaatccggcc aatgctcagg catttgcgca gctgaaaaaa gaagccgaca
     7261 tgaaagtgcc acgccgtcat gtagagctgt acgacaccat catgggttgt cttggctata
     7321 cttcaccgga tggtggaatt aacgaaaaca acaactggtg tcacatcccg cagacaaaag
     7381 tggcagcggc taactaagcg gcctgctgac tttctcgccg atcaaaaggc attttgctat
     7441 taagggattg acgagggcgt atctgcgcag taagatgcgc cccgcattcg gtgattggcg
     7501 cagcctggta gcgcacttcg ttcgggacga aggggtcgga ggttcgaatc ctctatcacc
     7561 gaccaaattc gaaaagcctg ctcagcgagc aggctttttt gcatttgaag cccatgagga
     7621 tgagaacctc cgggggcggg gaggttcgac tcgagcgaag cgagagaacg ttgcgccagc
     7681 aacggcccgc agggcgagcc acgaagtggc gagtaatcct ctatcaccga ccaaattcga
     7741 aaagcctgct caacgagcag gcttttttgc atttgtagct catgaggatg agaacctccg
     7801 ggggcgggga ggttcgactc
//
LOCUS       DAZFWL020000184         7767 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFWL020000184 DAZFWL020000000
VERSION     DAZFWL020000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.184"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(449..1101)
                     /locus_tag="ACHKNP_RS22415"
                     /old_locus_tag="pgaptmp_004483"
                     /pseudo
     CDS             complement(449..1101)
                     /locus_tag="ACHKNP_RS22415"
                     /old_locus_tag="pgaptmp_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(704..820)
                     /locus_tag="ACHKNP_RS22415"
                     /old_locus_tag="pgaptmp_004483"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            1839..1973
                     /locus_tag="ACHKNP_RS22420"
                     /old_locus_tag="pgaptmp_004484"
     CDS             1839..1973
                     /locus_tag="ACHKNP_RS22420"
                     /old_locus_tag="pgaptmp_004484"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706524.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            2574..3395
                     /locus_tag="ACHKNP_RS22425"
                     /old_locus_tag="pgaptmp_004485"
     CDS             2574..3395
                     /locus_tag="ACHKNP_RS22425"
                     /old_locus_tag="pgaptmp_004485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU4706525.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            3395..4501
                     /locus_tag="ACHKNP_RS22430"
                     /old_locus_tag="pgaptmp_004486"
     CDS             3395..4501
                     /locus_tag="ACHKNP_RS22430"
                     /old_locus_tag="pgaptmp_004486"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU4706526.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            4595..6316
                     /gene="cptA"
                     /locus_tag="ACHKNP_RS22435"
                     /old_locus_tag="pgaptmp_004487"
     CDS             4595..6316
                     /gene="cptA"
                     /locus_tag="ACHKNP_RS22435"
                     /old_locus_tag="pgaptmp_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU4706527.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            6390..7388
                     /gene="lpxM"
                     /locus_tag="ACHKNP_RS22440"
                     /old_locus_tag="pgaptmp_004488"
                     /gene_synonym="msbB"
     CDS             6390..7388
                     /gene="lpxM"
                     /locus_tag="ACHKNP_RS22440"
                     /old_locus_tag="pgaptmp_004488"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU4706528.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLIAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            7441..7629
                     /locus_tag="ACHKNP_RS22445"
                     /old_locus_tag="pgaptmp_004489"
                     /pseudo
     CDS             7441..7629
                     /locus_tag="ACHKNP_RS22445"
                     /old_locus_tag="pgaptmp_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2042 a         1729 c         1865 g         2131 t
ORIGIN      
        1 agcaagaagc atctaaaaac aaaaagctac cgcaaggaga atctctccca accaaaacaa
       61 cattagcgat aataatatat tgcctaaaca ttcttacaca tatcacagct aagttatatt
      121 ataacactca gatgattgat ttgtaagata acgtatgatt ttcatatgaa tagccacgtg
      181 acaaatgcct tattaaagag attgtaaaca gaatacagca aactatttca gttaccgaaa
      241 tgacgagtta atcagttttg tttaccctgc aaacacaaaa tctaaaccta cacagaaaag
      301 caaacctctc ctgttaataa taaatatctt ccggtatagc tggagttttt tcaaatgcgc
      361 ctataaggct ctcttaccag ccgagccata acaggcgcac acgatctgac atttgcatcc
      421 aacttcgtta cttacggccc gtaaatcggc tgacggtagt acggaccttt ttaccccgga
      481 gaacaccggc aagtcccata accgccatga ggcgcgccac tgtacatctg gccaccctga
      541 taccttcgcg taacaactgg cgccagactt tacgcacacc gtatacctga tgattttcat
      601 cgtatacgcg ctgtatctct ctcttcagcc agtcatcgcg ctgcgcacgg gcactgcgtt
      661 tatcaggatg atgtcgctgt tgctgacagt ggtaatacgt tgacggggca atatgcagtt
      721 cactgcatac cggtccgacc ccgtactgct cacgcagctt atccagcaga ggcattattt
      781 tttccagagg cggtcgaact ccgccttcgc aaaataagcg gaagcctggc gaaggatatc
      841 gttactgcgg cgcagttcac gattttcacg ttccagctct ttcagacgct gacgttcagc
      901 ggtggtgagt ccaccatcac cactcccggt atcccgctca tgctgacgaa cccacacacg
      961 cagagtctct ggtgtacagc caatctttgg ggcaatggaa caaattgccg cccattgtga
     1021 gtcatattcg ccctgacttt ccagaaccat acgaactgcc cgttgacgga cctcggggga
     1081 aaaacgtgta tttttagtca tcctgtttac ctctttctca gggagtttag tctccaggat
     1141 tcccggggcg gttcatatac ccctaattca actaattgga ggcattacca tgcattagtg
     1201 caagcgcctt gtggattctt ttgctaatgg cggaaacata ttgtacaata ttgcatttat
     1261 ccctcaataa ataaatgagt tatcagattg caaaaatctg ttatcaaact aattcaaata
     1321 aaaagagatt gatacgacca acgtacacaa aaattttcga tattcgaact aaataacagc
     1381 tcctaatacc gctacacttt agctatccag tctgcgcctc agaaatggac gatgccgttg
     1441 aaggactggt gtatggcaat gagccgcttt attatcgaat tcggtgaccg cttggacggt
     1501 cacttcggag aaaaggcatt tacacagcat cctaaacagg ttcagcatag gtgatatacc
     1561 gccttttcat tttcttctcc attatttata agggaggtgt attattatgc aatgttattg
     1621 gttttatcca ttgtttttta gtttatgaat gttcgcaaaa aaaataaatg aacaataatt
     1681 ttatgtattt aaaataaatt aattttactt ctgtaatggt gtcatgatta gcagcttttt
     1741 aaactaatat cgactttcaa taaataagga caggtaatgg acaacaaaaa tatctgaacc
     1801 cgggtgagat tcataagttg cacagtgctg tcttcaaagt gccacacccg gaacgtaatc
     1861 actgcctgct ccttatgggg tatcttcatg gtgtacaagc atcagagtta ttaggtataa
     1921 aactgtctga tattgatttg caggccggta acctgaatat ccggcggctc taaacggttt
     1981 gtcaactcag cccctcggct gaaacaggag gtacagtcca ttcgtcgctg gctgaaactt
     2041 cgggatgaat gggtagaaga gggatatgaa tggttattta acctctatgg caggtctgct
     2101 acaggtatta ctgttgttct ggggggcact ggtgtggtta atatctacga tgcaagagat
     2161 tctcacgatg gcccggtcca tcagtgcatg cggcgcgata ttgtgcgagt atttttcagg
     2221 gggagtggta tccggatgca gaaaattccg ctactcgact ggttttatgc aataaatcag
     2281 gatgataatc ttctgatttt ttactgaggc ggatatctct cgctgtgttt actgaaattg
     2341 cggtattctg ttttttctga aaaacagaaa atctgattaa aaagagcata tgcgttaagg
     2401 gaaatttaag ttctggttga ttaactgata aaaatattaa aaaatccctg ttgtcggtaa
     2461 tctccggacc attccgggat tacacaataa tatcaccatg tgtgatatct atggatcctc
     2521 actgttgttt acgcgaggat attttcagaa tgcttaacag acgataattt tttatgttga
     2581 atgcaaggca tcttcccgta ctgatgtatc atcatgtcag tcgttgtccg gggcttgtga
     2641 cactctcacc tgaaacattt tgtgaacaga tgaagtggct cgcagataat aactggcgga
     2701 cggtaacatc tgctgagctt gaatattttt atcagggggg aacgcttcca cgaaagagtg
     2761 tcatgctcac attcgatgat ggttatcttg ataactggct cagagcatgg ccggtcctga
     2821 aggaatataa ccttcatgca catatttttc tcattacggg gctgattggt aagggaaacg
     2881 tacgtagcag gcaggagcag gaatattctc accgcgactg tgaacggttg attgccgata
     2941 accgttcaga tgaggtcatg cttcgctggt ctgaagtccg ggaaatgcgt gattccggac
     3001 tggttgaatt tcatgtgcac acccattcac ataaacgctg ggacaggttg tctgtatcac
     3061 gtgctgaaca gtgccggctc atgaaagaag atattctggt gggaaaacaa tgtctgacgg
     3121 aaaagctggg gttctgtagt tcccatctgt gctggcctga aggatattat aatcgggatt
     3181 atattaatct cgccggtaag cttggtttct cttatttata tacaaccgaa agaagaatga
     3241 actgtcctga aaatggttca ttacgcatag ggcgtatcag caccaaagag cgggaacatt
     3301 ccggctggct gaaacgacgc cttttttatt atacaacgcc acttttttct tcagtactgg
     3361 ctcttcataa ggggccaaga ttaccagata attaatgatg aaaattttat tcactgaatc
     3421 ttcgtcagat attgggggcc aggagctgca ggcactggcc cagatgactg ctcttcaaaa
     3481 gcagggacat tccgttttac ttgcctgcag ggaaaaaagt aaaattgctc cggaagccag
     3541 aaaaagaggg catgatgtca cttttattcc tttcaggaac agccttcacc ttccttccat
     3601 cctcagactt cgacggatta tcggggaatt taaacctgat ctggtcatct gccatagtgg
     3661 ccatgacagt aatatagcag gactgagccg tctgatatgc tgtcaccgct tcagtattgt
     3721 cagacaaaaa acctacatca cccgtaagac ccgaacgttt tccctgaatt atctgtgtga
     3781 tttcattgtt gtgcctagct cagccatgat ggcgcatctg atggcggaag gtgtcaggac
     3841 accggtaacg gttattccac cgggtttcga ctggcccgca ttgcataatg aagccatgcg
     3901 accgttaccc ctgcatatcc acgcctgggc tgcatcagca gacaatgttc cgcttatcgt
     3961 tcaggtgggg atgctacgtc ccgaaaaagg acatgaattt atgctgcgtg tattgtatca
     4021 gctgaagatg gaagggaaat catttcgctg gctcgtggtg ggcgccgggc gtgaagaata
     4081 cgaagcacgt ctgcgtcagc agacagaaca cctgggaatg agcggagatg tgttaatggc
     4141 aggtgccctt ttcccagcgt taccggtcta ccggattgcc agtgtggtgg tcatgccttc
     4201 ggaaaatgaa gcgtttggta tggtactggc tgaagcgtca gtctctggcg tgcccgttat
     4261 cgccagtgag acgggcggga tccctgatgt cattcagaag aatgtgacag ggacgctgtt
     4321 acccgtgggg gatgtgtctg catggaccgg cgcactgcgc gattttttat cccgaccgga
     4381 acgcttccgg atgatggcag ccagcgccag agaagatatt gagtaccggt ttgatattaa
     4441 cagaacggca cagatcattg tgtctctggc tagtcaggct aaggggaaat gtaacagata
     4501 acggcggatc ctcttttcag cccgaatttt gttcagcggt tttccggcaa gtaacggagt
     4561 aatttatcac attgtttaaa tacggggacg gtcaatgcat ttaaataccg gacagaacag
     4621 gcctactttc agctggagtg cgctgggatg ggcaattttt tattttggct ttttttccac
     4681 tctcctgcag gtcatcatct tcagcagtgg gtacagcgga acgaatggaa tacgggactc
     4741 actgttattc agttgtctgt ggttgatccc ggtgtttctc tatcctgatc ggataaaaat
     4801 aattgcagct gttgtcggtt tcattctctg gggcacgtcg ctggcagcac tttgttatta
     4861 ttttctctat ggtcatgaat tctctcaaag tgttcttttc gttatgtttg aaacgaatgc
     4921 cagagaggct ggtgaatatt tcagccagta tttcagcctt aaactgttgc ttatctcgct
     4981 ggtatatact gcggtgtccg tttttctgtg gacacgtctg cgtcctgtat atattccttt
     5041 gccatggcgg agaattgtct ctttcctgct gctttatgct ctgcttctgc atccggttgt
     5101 tctgaaatcg ttaatcagac aggagccgct gaatgatact ctaggcaaac tggcatcccg
     5161 aatggagcct gctgccccct ggcagtttgt atccagttat tatcagtacc atcagcaact
     5221 gaatgcactg acaaccttcc tgaatgaaaa tagcgcactg ccaccactgg gtaatctcag
     5281 ggatgaatca ggggagagac cacgcacact ggtcctggtg attggtgagt cgacacagcg
     5341 cgaacgcatg agcttatacg ggtatctacg tgaaacgacg ccggagctgg atgcactgcg
     5401 taaaaccgat ccgggtctta ctgtgtttaa taatgtggtg gcatcgcgtc cgtacaccat
     5461 tgaagcattg caacaggccc ttactttcgc caacgaaaag aatcctgatc tgtatctgac
     5521 gcagccgtcg ctgatgaaca tgatgaagca ggcaggctat aaaacattct ggattaccaa
     5581 ccagcagaca atcacagccc gtaacaccat gctcactgta ttttcgcgcc agacggacag
     5641 gcagtactac atgaatcagc agcgaacaca aagtgcgcgt gaatatgaca ctaacgtgct
     5701 gaagccgttc cgggaagtgc tgaatgaccc tgcaccaaag aaactgatca tcgtgcatct
     5761 gctgggtacg cacattaagt ataaataccg ttatccggaa ggtcaggggc ggtttgatgg
     5821 cattacaggg catattccca ctggattaaa tgcgaaagag ctggaagtgt ataacgatta
     5881 cgataatgcc aatctgttta acgatcatgt ggtggccagt ctgataaagg acttcagagc
     5941 aactgcgccg gacggctttc tgctttattt ttcagatcat ggtgaagaag tatatgatac
     6001 tccgccatat aaaacgcagg gacggaatga agataacccc acacgtccca tgtacactgt
     6061 tccgttcctg ctgtggacct cggaaaagtg gcatgctgca catccgcgag atttttcgca
     6121 gtatgttgac cgcaaataca gtctggctga actgatccac acctggtcag atttggcggg
     6181 actgacatac gatggttacg atccgacccg ttctctggtg aatccgcaat tcagggaaac
     6241 tacccgctgg attggaaatc cgtataaaaa gaatgggctc actgatttcg acactcttcc
     6301 gtatggtgag ccatagtatt ttcttatctt cggttccgtt ctgaaggcag gaggatgtgt
     6361 tttcctctgc tgtccggtgg actccaggga tggtaccacc ttctgctgta ctctgttatc
     6421 ataatgagat aagcaggcag ataccggtaa atatgaaaaa tatcagaact gagtttattc
     6481 cccgctttaa ccttacatta tgcttccccc gttactggat gacgtggaca ggtatcggta
     6541 ttatctgtgt gtttgcaatg gtcccccccg cactgagaga tccattgttg ggaaaattgg
     6601 gcatgctggt tgggcgtctg gggaaaagcg cacgtcaacg ggccttgatt aatttatcgc
     6661 tttgttttcc ggagtacagt gataaagaaa aggagaatat cgttgatgcc atgtttgcaa
     6721 cagcctccat ggccgttgtg cttatggctg aattagcact gtccgggcct gataaaattt
     6781 cgcatcgtat acgctggaat ggtctggaga ttgtggagaa gatggcacag aacaatgaaa
     6841 aggtcatttt tcttgtgcca catgcctggg gagtggatat tcccgcaatg ctgatagctg
     6901 cgtcgggcag aaaaatggca gcgatgtttc ataaccagag aaatccggtg gtggattatg
     6961 tctggaacag tgttcgtcgt cgtttcggag gaaaactgca cgcacgtaat gacggtattg
     7021 cctcctttgt ccgctcagtc cggcagggat actggggata ttatcttcct gatcaggatc
     7081 atggcccgga attcagtgag tttgcagatt tttttgcgac gtataaagca actttaccgg
     7141 ttatcggacg gctcagcagg atcagtggtg cacggataat accgcttttc cctgtttatg
     7201 acggaaagac ccatcatctg actattcatg tcagcccgcc acttgctata cgacagaaga
     7261 gtgatgctca tattgcgcga cagataaacg aagtggttga aaatttcgtt cgtccacatc
     7321 ctgagcagta tacgtggatc ctcaagttgc tgaaaacgcg taaagaaggg gaagaggatc
     7381 cttactgagg caggtttttc aggataatga gcttacccag caatagtgga cacacgctaa
     7441 gtgagtaaac tctcagtcag aggtggctca tatgacaaaa aaacagtacc aaccagtaaa
     7501 aaaccccgta aatagcattc gcctgaattt cgcagtgaag ccctgaagct tgctgaacgc
     7561 atcggtgttg ctgtcgcagc ccgtgtgctc agtctgtatg aatcacagct tgtaagcggc
     7621 tcgtcagaac tgtattgata tttactgagc ggtcgtaagc gtacagcgag ggccgtattg
     7681 acggggatgt gttattcagc tggcagtgct atgcgccacg gaagcagttc gctgacccgg
     7741 ttgaccggcc agtctgctat gacgcca
//
LOCUS       DAZFWL020000185         7710 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFWL020000185 DAZFWL020000000
VERSION     DAZFWL020000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.185"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            403..1410
                     /locus_tag="ACHKNP_RS22450"
                     /old_locus_tag="pgaptmp_004490"
                     /pseudo
     CDS             403..1410
                     /locus_tag="ACHKNP_RS22450"
                     /old_locus_tag="pgaptmp_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2030..3539
                     /locus_tag="ACHKNP_RS22455"
                     /old_locus_tag="pgaptmp_004491"
                     /pseudo
     CDS             2030..3539
                     /locus_tag="ACHKNP_RS22455"
                     /old_locus_tag="pgaptmp_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3590..3704)
                     /locus_tag="ACHKNP_RS22460"
                     /old_locus_tag="pgaptmp_004492"
                     /pseudo
     CDS             complement(3590..3704)
                     /locus_tag="ACHKNP_RS22460"
                     /old_locus_tag="pgaptmp_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3791..4267
                     /locus_tag="ACHKNP_RS22465"
                     /old_locus_tag="pgaptmp_004493"
     CDS             3791..4267
                     /locus_tag="ACHKNP_RS22465"
                     /old_locus_tag="pgaptmp_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4706529.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACMAQIARENGVND
                     DVIFKWLRLWQNEGRISRRLPVTTTSDAGVELLPVEITPDEPKEPMAALTPSLSTQTT
                     VSASSCKVEFRHGNMTLENPSPELLTVKWHTEFGHLNRGDMLTSEQHRCSNEKKKF"
     gene            4248..5400
                     /locus_tag="ACHKNP_RS22470"
                     /old_locus_tag="pgaptmp_004494"
                     /pseudo
     CDS             4248..5400
                     /locus_tag="ACHKNP_RS22470"
                     /old_locus_tag="pgaptmp_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
     gene            5463..6912
                     /locus_tag="ACHKNP_RS22475"
                     /old_locus_tag="pgaptmp_004495"
                     /pseudo
     CDS             5463..6912
                     /locus_tag="ACHKNP_RS22475"
                     /old_locus_tag="pgaptmp_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6979..7471)
                     /locus_tag="ACHKNP_RS22480"
                     /old_locus_tag="pgaptmp_004496"
                     /pseudo
     CDS             complement(6979..7471)
                     /locus_tag="ACHKNP_RS22480"
                     /old_locus_tag="pgaptmp_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558155.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7394..7624
                     /locus_tag="ACHKNP_RS22485"
                     /old_locus_tag="pgaptmp_004497"
     CDS             7394..7624
                     /locus_tag="ACHKNP_RS22485"
                     /old_locus_tag="pgaptmp_004497"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGU4706530.1"
                     /translation="MNCWRCFPLFFIGILTTDEWGSHIRYVIEGDSSIFIQHIECMRI
                     LIKHLTRRAICFPHSVNFIKKHRYFIEKYMFY"
BASE COUNT         2054 a         1836 c         2043 g         1777 t
ORIGIN      
        1 gagtaggcag cctagcggct gcggcttgtc atggcctgaa attaccgtta taaaaacaaa
       61 caatgtcatt gtctttcagg tagttatatg gcacgttcag ctaaaccccg taaacgaaaa
      121 ctcgcacctc aaagaagcaa acttccccgc tatgttgtaa aacttcacga agatgatttt
      181 tttgacgaag aagaagtaga gattctgcgc ttcgataatt ttgacgatac tgttgagtgt
      241 tgcgcagacc tgaatattcc gttctttgtg gatgccggaa acaaaaagct ggtcttctgg
      301 tttgtacgtg ttgatgacga agggcatccg gaaatcgccc gctgtacaga gcgggagttt
      361 acgaccattc ttgccggtat cagcgccggc ggcatgtact gcccggactg ttgcaataca
      421 aagaaggtct gttttcggtg taaaaaacgc tcctgcccac actgcggggt gaaagcgggt
      481 gcacagtgga tacagtatct gctgagtctg gtccccgacg gcccgtggca gcaattgtat
      541 tcacgcttcc ctgccagtac tggtccctgg tgttccacaa ccggtggtta ctggcagaga
      601 tgagccgcat agcagcagat gtgaagccgg ggatattcac ggtaatccac acatggggcc
      661 gggaccagca gtgtcatcca catatccaca tatccattta tccatttatc gacaactgcc
      721 ggcggtgtga catcacatca cacctggaaa aaccttcatt tttacgcccg taaggtgatg
      781 agtatgtagc gataccggat aacacggtta ctgtcacgga aatacccgga actattgata
      841 ccggatgagc tggctgctga aggaagcagt aaacgggaat ggaatcgttt cctggacagt
      901 cattaccgac ggggcaggaa tgtcaacgta tcaaaggtga tggataacgc cacacatgtg
      961 gcggtgtact ttggctctta cctgaaaaag ccgccggtgc caatgagccg tctggagcac
     1021 tatgccggtc aggatgaaat cggactgcgt tacaacagtc accgcacacg acgtgaagaa
     1081 tacctattga tgagtggtga tgcgtttatg gaaaggttct cctggcatgt ggcggataag
     1141 gggttccgta tggtgaggtt ctacgggttc cttagtccgg cgaaacgccg gttgctggaa
     1201 gaattagtgt acgtaataac agagacggtg ataaaaacgg cgatgcaaat caggtggaga
     1261 gggatgtatc agcggttact gaaggttgac ccgctgaaat gcatcctgtg cgaaagtcag
     1321 atgcggttta cggggctgaa gcggggttac cgtctggcag agctggttat gatgcatgag
     1381 ccgctggccc gaatgcagta ttgcggctga gagccgcaga ggggaagttg cgtccatttt
     1441 gagggaaatg gagcaaaaaa acgtcaccga taccctgtat caatcaatgt cagcaatata
     1501 agcgctgata tatacccgat gaggttccgg atgtgttgaa taaatgttcg ttttttgttt
     1561 tcttaatctt tccgggggat aaattcctgt cgatgaacat atcaaaatca ggaaattaca
     1621 cgaatctatg tacaggctct ctgggaacac tttacgcttt gacacagagg gctaaataat
     1681 gagcgattca gatgccattt ttgcatacag aggattgaca gcattcccat gctaattgtt
     1741 aaggtttaaa ttgaagtata tctatctgtc atttttgcaa atgacatgcg gtagcataac
     1801 gttccgaggt ctcttatgca gacatacgtg gcgacattaa ccactcccag atacagccag
     1861 ccttcatccg tacgtaagta cgtgatgtct cgcgcccact tctggctctg gctactggcg
     1921 taaaaatcct gctcctgtca cgaacggtgc aaaagtggtt cttccgcaac ctgggtggag
     1981 aaaatggatg aaactttatg gtgaacaagg atctaagctg tttttagtta tgggtaacgc
     2041 aatgcactct cttaaaacac ttctacagtt accttgcgga tggcgatgta gtcgacaaat
     2101 tattaactct gacggtatac ccctccatct ccgcggaaaa cgcaaaacag cacaatgtcc
     2161 tgaatgcttt aagcgtagcg actctgttca tagttgtcgt cggcgccgga tacagcatct
     2221 accctgctca gggcagacgc tatggcttgt attttccgtc cgccacaggt acagccgtaa
     2281 cccggcttgt tcacgaaaaa tttttgccga gtcgtttgtt cccttcggtg gttcacatct
     2341 gcagtcttca caggcgttac aaaatttaca gcgtcaactg ggattaatcg ccggaggtga
     2401 ggccggaaaa cgggcagcaa cggcagtggg tctccgttgc agtgctgata ctctccttcg
     2461 cagggttatc aataccccgg agacgaaaca gtcaggcgcg cctcatgtcg gtattgatga
     2521 gtgggcgtgg catcggggcc accgttacgg tacgttaatc gtcaatcttg atactcaccg
     2581 tcccctcgtc ctgcttcccg gccgtgatca gcgtacgctg gcgacctggt tcagaaaata
     2641 tccggaaata cagattgtct cgcgtgatcg cagtggggtc tgtgcgacag cagcacgtga
     2701 aggtgtacca caggccagac aggtggtcga tgccacctgc taaaaaatat tggcgatgcg
     2761 cttgaacgaa tgatgtacag acatatacct ctgatacgtc ttgttgccag cgagttgtca
     2821 ccaaagaaac cacctgagcc agaactgtct gagcctgcag catcgctccg tcgtccggaa
     2881 cgcctaaaac aacaaacccg caaaaaacgg catcagcgtt ggacagaggt tatggcccag
     2941 cataacaagg gatgtagttt cagggaaata tcccgtatta caggcctgtc gcgtgtgaca
     3001 gtcagtcgct gggtgcgttc aggaacattc cctgaaatgt caacccgacc tccaaagcga
     3061 gggcttctgg acccatggag ggagtggtta aaagagcaac gagaaagtgg taattataac
     3121 gccagccgga tatggcggga aatggtggcc cgggtaagcc gctggttgat gccctggaga
     3181 ataaccaggg atgaagaaaa ttatgcttcc cgatttatta gtctgatgtg tgaaaagaac
     3241 ctgagctgaa aatagcgcag caactggcac tcgagttcta ccgtattctg aaaacccaaa
     3301 ataaatcaca gctaagtagt tggttcactc gagtccacga aagcggttca gcagaatttc
     3361 ggcgagtggc ggcggggatg gaagctgatg ctgcagctat atgtgaggca atcagtagtc
     3421 gctggagtaa tgttgttgtc gaaggtcatg taaatcgcct gaagatgctg aaacgccaga
     3481 tgtatggtcg agcaggattt gaactactca ggcagagggt aatgagtcca ctggcatgaa
     3541 aatctccacc caggttgcgg aagaggcact tttcatccgt cgttgacagc tccagcagat
     3601 tttctgacac aggcaggctg tgtgcggtaa gggctgaact tccgggaagc ctttgccctc
     3661 agtccgtgac ggcacaggct tgccatcacg gtttttacgt taaaagtaag cgtaaaccga
     3721 ccgcagtatg tagccattag acaagaattg gtaacttaga cgcccatctg acacagacgg
     3781 acatccaagt atggaattac aggactggcg aaaagaacct cgtaaaaagt attcgaatga
     3841 attcaaactt cgtatggttg aactggcatc acaacccggt gcttgtatgg cccagattgc
     3901 acgagaaaat ggcgtcaatg atgatgttat tttcaaatgg ctcagactct ggcaaaacga
     3961 agggcgtata tcacggcgtc ttccggtgac aaccacttct gacgcaggcg ttgaattatt
     4021 acctgtagag ataacgcctg atgagccgaa agaaccgatg gctgctctta ctccatcctt
     4081 atccactcag actactgtca gtgccagctc ctgcaaagtg gagttccgtc acggtaacat
     4141 gacgctggaa aatccttcac cagagctgct cactgtgaag tggcacactg aatttggcca
     4201 cctgaacaga ggtgatatgc tcacctcaga acaacacagg tgctctaatg aaaaaaagaa
     4261 attttagcgc agagtttaaa cgcgaatccg ctcaactggt tgttgaccag aaatacacgg
     4321 tggcagatgc cgccaaagct atggatgttg gcctttctac aatgacaaga tgggtcaaac
     4381 aactgcgtga tgagcgtcag ggcaaaacac caaaagcctc tccgataaca ccagaacaaa
     4441 tcgaaatacg taagctgagg aaaaagctac aacgcattga aatggagaat gaaatattaa
     4501 aaaaggctac cgcgctcttg atgtcagact ccctgaacag ttctcgataa tcgggaaact
     4561 cagagcgcat tatcctgtgg tcacactctg ccatgtgttc ggggttcatc gcagcagcta
     4621 cagatactgg aaaaaccgtc ctgaaaaacc agacggcaga cgggctgtat tacgcagtca
     4681 ggtacttgag ctacatggca tcagccacgg ttcggccgga gcaagaagca tcgccacaat
     4741 ggcaacccgg agaggctacc agatgggacg ctggcttgct ggcaggctca tgaaagagct
     4801 ggggctggtc agctgtcagc agccgactca ccggtataaa cgtggtggtc atgaacatgt
     4861 tgctatccct aactaccttg aaaggcagtt cgccgtgacc gagccaaatc aggtgtggtg
     4921 cggtgatgtg acctatatct ggacgggtaa gcgctgggcg tacctcgccg ttgttctcga
     4981 cctgttcgca agaaaaccag tgggctgggc catgtcgttc tcgccggaca gcaggctcac
     5041 catgaaagcg cgggaaatgg catgggaaac ccgtggtaag cccggcgggg tgatgttcca
     5101 cagcgatcag ggcagtcatt atacgagcag gcagttccgg cagttattgt ggcgatacca
     5161 gatcagacag agtatgagcc ggcgcggaaa ctgctgggat aacagcccaa tggaacgctt
     5221 cttcaggagt ctgaagagaa cgaatggatg ccggtggtgg gttacgtaag cttcagcgag
     5281 gcagctcacg ccataacgga ctatatcgtt ggatattaca gcgcactaag accgcacgaa
     5341 tataacggtg ggttaccccc aaacgaatcg gaaaatcgat actggaaaaa ctctaactag
     5401 gtggccagtt tttgttgacc acttcagaaa gccagttccg tcagaaagac cagcaactaa
     5461 gtctggttga agagacggag gccttcctgt gctctgcact ggcacgtgcc gaagaaaaga
     5521 tcgaagaaga tgaacgggaa atagaacatc tgcagactca gatagaaaaa ctgcgccgga
     5581 tgctgttcgg tacccgttct gaaaaactgc gtcgtgaagt tgaacaggct gaggccctgc
     5641 tgaaacaacg cgaacaggac agcgatcgtt acagtgggcg ggaagacgct ccacaggttc
     5701 cccgccagtt gagacagccg cgccatcgtc gtccgttacc ggcacacctt ccccatgcaa
     5761 tacaccgtct ggagtctgaa gaaagctgtt gcccggagtg tggcggtgag ctggattatc
     5821 tggaggaagt cagtgcagaa caactggaac tggtgagcag cgccctgaaa gtgatccgca
     5881 ctgttcgggt aaaaaagcct gtacaaaatg tgactgcatc gttgaagcac cggcgccgtc
     5941 ccgcccgata gagcgtggta tcgcgggccc cggattactt gcccgcgtgt taacgggaaa
     6001 atactgcgaa cagctgccac tgtatcgtca gagtgaaatc tttgcccgac agggtgtcga
     6061 actgagccgt gcattactct ccaactgggt tgacgcgtgc tgccagttaa tgactccgct
     6121 gaatgatgcc ctgtaccgtt atgtgatgaa tacccgcaag cttcacactg acgacacacc
     6181 ggtaaaggta ctggcactgg gcctgaaaag acgaaaacag ggcgcatctg gacgtatgtc
     6241 cggaatgatc gcaatgcggg ttcgtcatct cctccggcgg tctggttcgc gtactcaccg
     6301 aaccggcagg ggaaacaccc ggagcaacac ctccgccccc tgcaggcgga tgcgttcaac
     6361 ggttacgatc ggctgttcag tgcagaacgt gaagatggtg cgctgacaga agttgcgtgc
     6421 tgagcccatg tccggagaaa aatccacgat gtatacatca gcagcaaaag tgcgacggca
     6481 gaagaagccc tgaagcgcat cagtgaactg tacgccatcg aggatgaaat acggggatta
     6541 ccagagtcag agcgtcttga agtcaggcag cagcgaagca aagcgttact gacgtcgctg
     6601 catgaatgga tggtggagaa gaatggcacg ctgtcgaaaa aatccagacc aggcgaagcg
     6661 ttcagctatg tactgaatca gtgggacgcc ctctgttatt acagtgatga cggtctggcg
     6721 gaggcggaca ataacacagc ggaaagagcg cttcgtgccg tctgtctcgg gaaaaagaat
     6781 tacatgttct tcggtagcga tcacggcggc gagcgtggag cactgctgta cgggctgatc
     6841 ggcacctgcc gtctgaacgg tatcgatccg gaagcgtatc tgcgccatat tctgagcgta
     6901 ctaccggaat agccctccaa ccgtgttgac gaactcctgc catggaacgt agtactcacc
     6961 aataaataag cgtcaatacg gtgctccgta gcgctgtttt gaccggataa aagccgcgag
     7021 taccacgctg tcgcagtgct gacggaactg ctgacatgta cttatccttg tccgccgctg
     7081 acaccacata taccagtcac tgcggccttc ctggagcaca tggcacattg ccttgatgtt
     7141 gaacacagcc tgatgttttt caataaagac atacttcatt tcaggcgctt cgcgaagtat
     7201 gtcgcggcct tttggaggat ggtcagctct tcatcccgtt ccgccagctg gcgtttgaga
     7261 cgtgcaacat cggtagacat ctccagctca cgttcagaag aacgtctact gattttgttg
     7321 ttgactaccc agttgtagac tgtgattcat acaggctgag ctcacgggct gcagcagcaa
     7381 tacctatact gttgtgaatt gctggcgctg cttccctctt tttttcattg gtatattgac
     7441 cactgacgaa tggggtagtc acatcagata tgttatagaa ggagactcat ctatttttat
     7501 tcagcatatt gagtgcatga gaatattaat caaacatctg acccgtaggg ctatctgttt
     7561 cccgcactct gtaaatttca taaaaaaaca ccggtacttt atcgaaaaat acatgttcta
     7621 ctaattggat atcaccaaat catataaaat aatgtgtttt atataacaac ctcaaaatac
     7681 atttgaggtt gtcaccaatc aaaattcatt
//
LOCUS       DAZFWL020000186         7624 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFWL020000186 DAZFWL020000000
VERSION     DAZFWL020000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.186"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(289..1050)
                     /gene="envY"
                     /locus_tag="ACHKNP_RS22490"
                     /old_locus_tag="pgaptmp_004498"
     CDS             complement(289..1050)
                     /gene="envY"
                     /locus_tag="ACHKNP_RS22490"
                     /old_locus_tag="pgaptmp_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HGU4706531.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1233..2123)
                     /locus_tag="ACHKNP_RS22495"
                     /old_locus_tag="pgaptmp_004499"
     CDS             complement(1233..2123)
                     /locus_tag="ACHKNP_RS22495"
                     /old_locus_tag="pgaptmp_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HGU4706532.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2124..5096)
                     /gene="nfrA"
                     /locus_tag="ACHKNP_RS22500"
                     /old_locus_tag="pgaptmp_004500"
     CDS             complement(2124..5096)
                     /gene="nfrA"
                     /locus_tag="ACHKNP_RS22500"
                     /old_locus_tag="pgaptmp_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HGU4706533.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAAQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5083..7320)
                     /gene="nrfB"
                     /locus_tag="ACHKNP_RS22505"
                     /old_locus_tag="pgaptmp_004501"
     CDS             complement(5083..7320)
                     /gene="nrfB"
                     /locus_tag="ACHKNP_RS22505"
                     /old_locus_tag="pgaptmp_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HGU4706534.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMFNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
BASE COUNT         1788 a         1961 c         2020 g         1855 t
ORIGIN      
        1 gcctttaaag gcatataagc gaaaatagca tgaggtttat cctcaattgc tatgtttttt
       61 agtacaaaaa agagggacaa aactgagaca cataaggcct cacaatggct tgcaaggctt
      121 tacatgtttt gaggtagtgg gacgtgtgag cgcagagatg gcgcggtaat ttgttgactt
      181 aaaatgtcgt tctaggaact tctaagtcgt gggccgcagg ttcgaatcct gcagggcgcg
      241 ccatttcttc ttcatttatg cccgtcttat ccgtttccgc tttgcccttc accacatcac
      301 tttttgtcgc tgtttggcga gataattcaa cggtgtcagg ccgtaaaacg ccttaaaaac
      361 agagataaag tacgacgtgc tgctatagcc acataattgc gccacctgag tgatattttt
      421 gttatccatc aataacatct gtacggcgta acgcatacga cactctgtga caatctggct
      481 atagctggta ttttcgtttt ttaatttctt tttgagtagg ctggggctta aacatagcga
      541 actggcgaca attcgcagat tccagtaatg ctgaatatcg ctttgaataa tgcggcagac
      601 gctgtcgcgg acgctgctgc gtaagatata catcagtagg gctataaacc gcgattgctc
      661 aagaaagtta gacaatacgg taaaaagtaa tgcgcgcgtc aactccgctt cgcagggttt
      721 gctgggttgc tgcttgctgt ggttggcggc tagccggaac acttccggtg tacggctatg
      781 acaagcgata accggggttg ccagccgcga ccagggcttt acacaggtta agttcttatt
      841 taaaaacaac agataatcgt tgatgatgtt acggttgatg tgggggatta atacgtgatt
      901 taacgttgag agttcaatga cgttattgtt gcacgcgaag gcggccaggt agtttttcgg
      961 aagggtaaac gtagcatggt tattgacgct tacctctacc tctttttcgg tcaggatcac
     1021 cacgcaaggt tcactgctgc tcaattgcat ttcgcactcc tcagatatca gaaactccgc
     1081 tcaaaggatc tatgcttcct gcatgagtga tcggcccgtt cgccgataac gatcttcttt
     1141 ctttagcacg ctttttagca attaatcttg atggaattct gacgagagcg aaagaggtaa
     1201 gccaggtcgt acccgactta cctggaggag atttaatact cgagaatgcc gtgcgcgacg
     1261 ggcaaatagc gcagagagaa atagagagtg tctttaccgc aagaggaacg tttcgctaac
     1321 agcgaggcga tctctgcatc cggtttcggt ttcgctgtaa aggttttgcc tttgccggcg
     1381 acaaacagtt cataaacaat gccgctggca ggggcgggac tttggcaatc ggcgctggcc
     1441 tgtagataac gttcacgctg ttcagcggta agtttatcca cgccgctgcc atcctgtacc
     1501 cagacattaa cgccggttgc tttaacttgt tccagcagtt ggcgatagcc atcgggcgac
     1561 atgtttccgg cgaaaaaact actgatataa accggttttg ctgaaacatc gctaatcagc
     1621 cgctgcgcgt tgtttaacca tgttagcaaa ggctgacggg cagcttcgct gcgccagttc
     1681 aggtcgtcaa tttccgcgct gatgtaccag ccatccggcg ttacgccagg cgcggcgctc
     1741 cataatctgg cttgctggag atcggcagcc agcaggcgat taagatagct ttccagcgct
     1801 gcggacgact gtttctggtg cataaaaaat tccggatcgg cgttcagccc gacaatcagc
     1861 ttcaggccag cctgttgcgc agctgcggca cgcttaaaca ataacgcgcg ctgttctggc
     1921 tgggtgaatg catcgccgta acgggtccat tgcaaaacca gggtatcgaa gccttgcaaa
     1981 cgtaactgac tcatcagccc ctgccactgg gtatcggtaa cctgactatc tcggttttgt
     2041 ggttgccaga taataccttt catcgcaaag gaaaaagggc tgaccaaaag cagtgtcagc
     2101 aatacgaaaa tgaacttacg catttaccag tgcactccaa tggtgagaaa cgcgttgttg
     2161 cgctctccgt tacgttgatt aatcgcctta aaggtatgtt gatactcgac gccgagactg
     2221 actttgtgcg gccaggcgtc gtagtgcgtc tcgccggtcc agatattcca gcggaccccg
     2281 actccgccaa gctgcgcgcc ctgagtgcct ttatcacgat agccgttgtc ctgaacgtga
     2341 gcgtaaggct caatagtctg tccgttagct accttctgat gccagctgac gcgataatct
     2401 gccgtccacg cctgaatatc ctggcggata tattgcgccg catcgaggta caggttttgg
     2461 gcaaaccagc ctgaaccgtt cgggtgccat tcgtcgctgt atttgccgcc attaaagaat
     2521 gaggcgctgg cgcgcagcat ggtatcggat gcgccatttt ggccgttcag cggcaactgc
     2581 tgttcgacgg cgaggaaaaa gatctgatcg cgcagcggct tccagcgcag accggtgccg
     2641 gacatcggat ttttcaccgg catcatcacc ccgttttctc cggtatcggc aaagacgcgg
     2701 ctataaactg agagcaggtc gccttccagc agcatattgc gtccgatgcg gtactcggct
     2761 tccagttgcc cgtagctacg atagcttttc cctggcgctg cgccgccgac attattgtta
     2821 gcggtgctca ttgccccgga acgcaagccg atagaagaat cgaaactgaa cgtccagcgg
     2881 cgaccgacct cctcatgcaa acggcggaaa ttgaagcgtt gctgattttg ctctggggtc
     2941 agtggggtta tcagcgcctg attatcaatg tcatcaatca ccagccgggc gtagtgctgc
     3001 gtcgcaggca tgtcatccag acgctggttc acgtaggcca gttgtcggat cagtgccgga
     3061 tcgtccggaa gccctttatg cgcctgttcg agcatttccc gcgactgtgc gatatcaccg
     3121 ctatcccaca aggcgtaacc aagcgctgcc tggatgttgc tattattcgg ttccagttcc
     3181 agcgcggcgc gcaaatcact caccgccgcc gggacattat gacgttggcg ataaattgtc
     3241 gcccgcgcaa cgtaagcgtt ggcagaaggc gcaatattga ttgagcgcgt gagatcgctc
     3301 agtgcgagtt ccggctgacc aggaatgtaa cgttgcgcat gcagccacca gtagagggca
     3361 ttgtttccca gtccacgttg ttctgcctgt tgtagccagc gatcgcgagc tgcaccattt
     3421 cctgccgcct gggcggtatt ggcagcagca agcagatcct cattgctcat gtcgtgaaga
     3481 ctgattttct gccaggccgc cagtgcggtg gcgtagtcct caacctgata cgcctgatag
     3541 gctaccgcac gatgttgcca ggcgttcggt tgtcgttgtt cggcctgaag ccatgcatac
     3601 aaggccacac cgggtagcgt gtcccgataa cactttgcca gacggttcca ggcggcggca
     3661 tcgtaggaag gcgacatatc gcccagcaag cgaactattg ccgggcaatt atctgcaata
     3721 cccggcaact ggctttgcca ctgacgttgc gccgccagcg gcaagggttt tgataaaatc
     3781 gccaccttcg ctggagttgc caggtaagga tgactttcca gcagagacgc cagtcgcgcc
     3841 attaaagtct ggctgacacg cgcatcgccc tggaaaggat agcgttgcag caataaatcg
     3901 gcagcttcgc gtgactgctc gttctgcatc agttgccagg tcagttgatc taggcgggta
     3961 agatttgccg gttcttgctg atacagcaat cgtgccagac gcagagattc agccttgtta
     4021 cgggtcgcca cgctgacagc ataacgctcc tcaagcattt cattggcggg gagggtggcg
     4081 agcagttttt gcgctgcgtc gtactgacct tcttttaaca gcaccggtag cgtcgcgcca
     4141 acaacatact ggcggttatc ggcaaactgc accgtataat tcgccaatgc ctgaacgggg
     4201 ttggcgctgt atttagataa cagatagagc caacttttct cttgtgcgtc cgtggtaaac
     4261 agcggcttat tttcaatgag ataatgctgg aggcgtgctt tttcgccacg ataagccagc
     4321 gcggtcgcgt aagtaatata tgactgagga tcggtgaaga tcccctgtga ttgcagtgcc
     4381 aggatccgat cgtccagctg cccggcaaga agcacgtcaa accactgacg gcgttctgcc
     4441 gcgcttaatg tgttctgctg gcgtgcttca ttgtatagcg tatctgcctg ggaccattgt
     4501 ttcaggtaga ttgcccgttg cagcagatcg gttcgcagcg tttttccttc cggcgatgca
     4561 gcaaacgtcg catcgttcag ttgcgctctg gcgacaggta actgtgccag ccgcagggca
     4621 ttctgcccga cttcactgcg acaacgcagg gtcggcgcag catcgcacgc tttttgctgg
     4681 gcaagcagtt cttcaacagt cgtaacgctt ttcacttcaa ctggaatagc cgccagactg
     4741 cgctcaagtc gggcatctcc tgggtgacgt ttcagttgat cctccagcaa cagccgcgcc
     4801 cggtcatcat gaccaaaatg gcgataggct tccgcaaggt ataaagtcag cggaatatta
     4861 tccggcacct gctggtgtat atattcaaat tcgcggatgg cggttgcttc gtcgttattt
     4921 ttctgtgcct tcagcgcctt atcgagacgg ggataaataa caaaatggcg ataatcgctc
     4981 agccccagct cttctgcgct ggtgccgata ttgtctgcga gtgcgctggt actcaataaa
     5041 gacgtcagca gtaaaccaga ccatccgatg acgcgattaa ggttattctc cttcattttc
     5101 ggactccagt tgcgcaacct gttctgtgtt taaacctgct ttgagtaata gtgattgcat
     5161 cgaaacttgt aattcgcgtt gaattgtcag gacgcgatcc aacgtttcct ggctgataac
     5221 gccttcggtg accaaaaact tgccgagcgg cagagaactg cgttcatggc gcaataacaa
     5281 cacgttaatt gctgaacgat taatatgacc gagcgtggtc agtatttcgg cgaacaggaa
     5341 ctgatgcggc acatattgcc gccagatttc accggtctgc tgttccgtga gccactgatg
     5401 ctgaaccgca ttgtacaaca ttgcccgcgg atcgtgaccg cgtcggcgtg cataccagtg
     5461 acgtaacccc gtgacaattt gtccccgcag aacaatgacg taacgcactt tgcgtccgac
     5521 tttacgcgtc agggccgcca gcgaaacagg gtcaatacca tcttcactgc cgacaattaa
     5581 ctcatcattt tccagacgca gcggcagtac tgcataatgc agcgctacgg aggctggcat
     5641 ttcggcaatc agcgaggacg ggatctgcca ggcatcgatg gattcccacg ccacgccgtt
     5701 ttgctctgcc agcgcctgtg ccagctgctc ggcgctaatc agcccctgca tcagcattga
     5761 accgcccagg cgtagacctt cgacgcgatt acgcagtgct gtatcgagtt gttcttcagt
     5821 gatgacctga ttttccagca gaatttggcc taacgggcgc aacgagcggg tatcgccagt
     5881 cacgctgggg aagtcatgcg ttgttttatc ccacgcgacg cgacgtggat cgccgtgttg
     5941 aagtacctgt tttagcgcgc gccagttggc catgaagtta atcaggttgc cccagaaaag
     6001 acgcaggaca gatagcagcc cctgcgtcag accgtagtag ccagtgacga aaatcacccg
     6061 ctgcacgatg cggttaaaca tcaagccaaa gtttagccac agcagggtca ttaaccatgc
     6121 gctgccgcta aaaatagaaa ggaaatgcca ggcatcgggc cacaaacttt catacgccag
     6181 caacagcaaa agctggagca tcaccagcat cgcgaggaag ctgacaaagt tactgattgc
     6241 ccctttgcgg tcgcgccaga gaaagtagtt cagcgtcagg ctggaggtcc atttgtgggt
     6301 tttaaagccc tggaaaacaa tgccgatgat ccagcgagat ttttgtcgaa ctgcagtcga
     6361 aaaggtatcg gggaaatatt cgcgcacgca gatcatgttt gacgtccgtg cgtgctgtaa
     6421 aaatttacgc tgctcgcgtt ctttggcttc gtccaccacc ggaaaacgga caaaaatttc
     6481 cgtcatacct ttctctttca ggcggaaacc aatgtcgtaa tcttcagtca gactctgcac
     6541 gtcgaaagca ataccgtcac cgtcagctaa cagagcggtc acggcgcggc ggctgaaaca
     6601 ggtgccgacg cctgcgctgg gcacttgtcc ggcgagggct tcacgcaccg gaacatcttt
     6661 gccatgcagc tctgaaaact catcaatgta agtcatgctg gtgaagtgcg tccattcgcg
     6721 ttcgaacgga tacaccggga tctgaatcag atctttacgc tcgaccagat agttgaacag
     6781 acgcaattcc atcggtgaaa tcacatcttc ggcgtcatgc agaataaaac cagcaaaagc
     6841 gaaattggcg ctacgttcaa actgggtgat ggcgtccagc acgttgttca gacagtcggc
     6901 tttgctggtg ggaccaggac gcgcgcagac taccttatgc acgttcggaa agcgagcgca
     6961 cacttcgtca acatcacgtt gagtatcggg gtcgttgggg taggtgccaa caaagatatg
     7021 atagttttcg tagtcgagcg tggtcgccgc cagctcggcc atattgccga tgacgcccgt
     7081 ttcattccac gccggaacca taatcgctaa cggtttttca tctggtttat acagttcgcg
     7141 gtaactcatt cgcgggtagc ggcgataaac actcaacttg cgtttaatgc ggcgtaccca
     7201 gtagacgaca tcaataaaaa aatcgtccag cccgctgatg aacatgatga ccgctaacgt
     7261 tatcgcgatt acttttaagc cgtagagcca ggttgcaaaa acatcaagaa gccagtccac
     7321 acaaaaacct tacattaacg ctggttatgt ttagggtggc gtatattaag gttttttatg
     7381 aattgtgaca gctttttacc attaataggt atgactattg cggcacgtta tttttacact
     7441 ggttataaaa gttgccgttt gcgtagaatg tagacaaaag catggtgtca aggaggggag
     7501 gttaacgtca tagtgaaaag gagcggtggc accgagcagg tcataatgta gtggaagtgc
     7561 ctggaggagg tgagcaggca gggcaagatt acgaaagccc gctccccgca aggactgacg
     7621 ccag
//
LOCUS       DAZFWL020000187         7542 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFWL020000187 DAZFWL020000000
VERSION     DAZFWL020000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.187"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(84..617)
                     /locus_tag="ACHKNP_RS22510"
                     /old_locus_tag="pgaptmp_004502"
                     /pseudo
     CDS             complement(84..617)
                     /locus_tag="ACHKNP_RS22510"
                     /old_locus_tag="pgaptmp_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(622..786)
                     /locus_tag="ACHKNP_RS22515"
                     /old_locus_tag="pgaptmp_004503"
     CDS             complement(622..786)
                     /locus_tag="ACHKNP_RS22515"
                     /old_locus_tag="pgaptmp_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706535.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            complement(783..1516)
                     /locus_tag="ACHKNP_RS22520"
                     /old_locus_tag="pgaptmp_004504"
                     /pseudo
     CDS             complement(783..1516)
                     /locus_tag="ACHKNP_RS22520"
                     /old_locus_tag="pgaptmp_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(1550..2011)
                     /locus_tag="ACHKNP_RS22525"
                     /old_locus_tag="pgaptmp_004505"
     CDS             complement(1550..2011)
                     /locus_tag="ACHKNP_RS22525"
                     /old_locus_tag="pgaptmp_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4706536.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(2004..3044)
                     /locus_tag="ACHKNP_RS22530"
                     /old_locus_tag="pgaptmp_004506"
     CDS             complement(2004..3044)
                     /locus_tag="ACHKNP_RS22530"
                     /old_locus_tag="pgaptmp_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HGU4706537.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(3016..3567)
                     /locus_tag="ACHKNP_RS22535"
                     /old_locus_tag="pgaptmp_004507"
     CDS             complement(3016..3567)
                     /locus_tag="ACHKNP_RS22535"
                     /old_locus_tag="pgaptmp_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4706538.1"
                     /translation="MKITTEQVCEALDTCVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGSYEYRCTTMG"
     gene            complement(3551..3778)
                     /locus_tag="ACHKNP_RS22540"
                     /old_locus_tag="pgaptmp_004508"
     CDS             complement(3551..3778)
                     /locus_tag="ACHKNP_RS22540"
                     /old_locus_tag="pgaptmp_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU4706539.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            3855..4262
                     /locus_tag="ACHKNP_RS22545"
                     /old_locus_tag="pgaptmp_004509"
     CDS             3855..4262
                     /locus_tag="ACHKNP_RS22545"
                     /old_locus_tag="pgaptmp_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4706540.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            4527..4826
                     /locus_tag="ACHKNP_RS22550"
                     /old_locus_tag="pgaptmp_004510"
     CDS             4527..4826
                     /locus_tag="ACHKNP_RS22550"
                     /old_locus_tag="pgaptmp_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706541.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            4899..5117
                     /gene="ydfC"
                     /locus_tag="ACHKNP_RS22555"
                     /old_locus_tag="pgaptmp_004511"
     CDS             4899..5117
                     /gene="ydfC"
                     /locus_tag="ACHKNP_RS22555"
                     /old_locus_tag="pgaptmp_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGU4706542.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            5140..5547
                     /locus_tag="ACHKNP_RS22560"
                     /old_locus_tag="pgaptmp_004512"
     CDS             5140..5547
                     /locus_tag="ACHKNP_RS22560"
                     /old_locus_tag="pgaptmp_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706543.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(5525..5758)
                     /locus_tag="ACHKNP_RS22565"
                     /old_locus_tag="pgaptmp_004513"
     CDS             complement(5525..5758)
                     /locus_tag="ACHKNP_RS22565"
                     /old_locus_tag="pgaptmp_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706544.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
     gene            6319..6507
                     /gene="dicB"
                     /locus_tag="ACHKNP_RS22570"
                     /old_locus_tag="pgaptmp_004514"
     CDS             6319..6507
                     /gene="dicB"
                     /locus_tag="ACHKNP_RS22570"
                     /old_locus_tag="pgaptmp_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4706545.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLELGIIGEQQ"
     gene            6504..6692
                     /locus_tag="ACHKNP_RS22575"
                     /old_locus_tag="pgaptmp_004515"
     CDS             6504..6692
                     /locus_tag="ACHKNP_RS22575"
                     /old_locus_tag="pgaptmp_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4706546.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
     gene            6788..>7542
                     /locus_tag="ACHKNP_RS22580"
                     /old_locus_tag="pgaptmp_004516"
     CDS             6788..>7542
                     /locus_tag="ACHKNP_RS22580"
                     /old_locus_tag="pgaptmp_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4706547.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFIPVEPESDPM
                     NVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEI
                     NAMYPERKLFAIGWVKHKCNPGAKWPEIQAELRNWKKRQDAERKETGKYTSVVDLASA
                     RVNQQNTENSAGKINPVTAAICREYKQTWKTLD"
BASE COUNT         1945 a         1907 c         1682 g         2008 t
ORIGIN      
        1 tatccgactt tacctcgtgc atagcttgtt atttaaagtc aacaaaataa ggaaaattat
       61 acgcatattg aagagtataa accttacatg ttgattacat ttttgtaatc aacatcctgt
      121 ttggaatagc cagcctttaa tggataacta tttctgacaa tgcaatgagt ataatcaagt
      181 ccatcttcca ctgagaatta gaggcggcat gctttttccg gctcttgccg gatatccgta
      241 attgtccata atctgcagat ttataccttc tgcatgacct gtcagcgaaa atttgtccgg
      301 tgtttctacg ggaatgacat caaaagttac acgcactcgc gttaccgtgt agacctactt
      361 tcctgcactt gcaagatcac agtggtgtaa ccgtaacagg aatttattct ctggaccggc
      421 agtaaatccc tgagtggcgt ggttcccata tcaatttccc gccaggcagc ctccattgcc
      481 agcgtacagg ctggagccat gacctgccct ttaaatctgg cccgaccatc ccaccggacg
      541 tgttcttctc ccctgaactt aggtacagtc atctccagtg gcacaaaagt gtcagcgcca
      601 taatttttga ccgttatcgc gctacggata ttttgttgac tggtgaaaat caccccgcag
      661 aatcaggctt attcccttaa cctgggcttt catcctgacc gccgcctcac tacgaccaat
      721 cagactgccg atgcatttta ccttcattgt tagtatcatg atttcaggcc tgcaccatcc
      781 gctcattgcc cggacttccg acaaatcccg gcaaccatat cccggtgctt gttcagctcc
      841 cgcagcgcgg cgcagactcg ctcccacttc tggacatgat tcttcgccct acgcagttcg
      901 cgatttgcca tatgcagcga tggcaaaatc aaatcatctt ctcgcgttgc agtaaacgat
      961 ggcagcgact gcacaatgtc cgccacagtt tctgttttaa tatcttcctg tgttgcatct
     1021 ttctgtaccg gtaacgaaca ccggctggct gaggaaaggc tttaccatcg gtttccgcta
     1081 ccgattcaac tttcggctct gctggtaaat tatcacctgg tatgcagtaa cgaattttac
     1141 cgttctggtt tacgcgaatc agacgacctt tgctgattgc cattgccagc gttgaagcaa
     1201 ctttgcggga tgttgtaccg aacagcgtag ccagttcatc cgccgtttgt ggtccgcgtt
     1261 gttcaatcgt cgcggttaaa tcgcactctg agattttcgc ttctgttgcc gtggtggttt
     1321 cttctggcgc tggctgttcc tgctgaacgt tgttatcagc cacacgccag gtgtatacgc
     1381 ttttatcaac gaagccagcc tttttcagtt cccacagctc gttcagtact tcttcacgac
     1441 tgatatcaag tcgcgcagcc agctctaccg acgtggcttt tcccatcgct ttcagtgcgt
     1501 caaaaacagt ctccataaat ttcctcccgg taaaaaatca cttctcaact caaacaaaac
     1561 cagccgcttt ccggcgttca tattcctgtt tcagcaactc aattggcgtt ggccccgacg
     1621 ggcgtttggg ggccgccagt tgtcgccgga ctggcggaac gctcaggccg ttactaacat
     1681 gctttgccca tttcgtcagt tgccgttctg caagccgttt taattcccct tcggtcatct
     1741 ggcgctcaat cccctttgaa cgcatctcga ggcaaatgtg atacagcaca ggctgagacc
     1801 acgggtactt atcacttccg tcgtatcgcc aggactcatt gcgccagcgg cggtactcct
     1861 ccatcacagc atccaccgtc agaccgaatg gattggctcc gcttttcgaa atcagtgcca
     1921 caaactcagc caggtccgga ggccatgttt cacccgcccg gcagcggtcc atgcactggc
     1981 ggcagacctg ccggatttgc tgctcagtca tcgcgccaat ctgtgcaatc cagagcttcg
     2041 aaggtgcggc cccgttcttc tggatccagc ggttcgaata aaccaccccc atgagttccc
     2101 acagcttcca ggccgtttcc gtcgctgata aatccgtttt cacgttccca ctgctcacgt
     2161 gctgcccgaa tttcctgaac tgcccgtgat gcggtgccac ctggtgctgc tgcatggttt
     2221 acccctttgc tgactggttt aacctgcgcc ctgacgtgat ttacgtgacg ggcgaatttc
     2281 tgctcccact gaatctgcgt aaacactttc ccctccgctg cccagtagtc ccggaaggcg
     2341 gcaagttcag caggtgtaaa ttctgtctcc ggcaaagcca tcccccacaa cgcagcccgt
     2401 cgtcgaaaat cccgtgacgg ataccagcta tcgctcatcg ggaattttcc gatgggttcg
     2461 ctcaggccat acaggaatac agggggggct gcctgtaacg acaaaacttc ctgctcactg
     2521 gtcggagcac tctcgcgtgc gttatgtgtg gggtttagat ctttgggttc ctttgggttc
     2581 cgtgatccgt ttttgggtgt ctttgatgga aaatttgggt gtctttggtt attttccatg
     2641 cagctaagag ttctgttttt gggtctgttt tgtgctgaaa cataaccatt ttcggtactg
     2701 tttttattaa cagcaccaat tttacccacc tttaaagact cccgtttttg ggtgtattca
     2761 ggctcggcaa cactttcttc tacaccgata agtcggtaca ccacaatttg ctttgttctg
     2821 ccttttctct caccggtatc aacaattaac ccaatctcca tcaggtgtcg taagctgtcc
     2881 tgcacagtct ttttgttcag ttccgttact tctgccagtg cagatacaga cgggtatgca
     2941 cacaaatcgg caccgcacat atcagcaagc caggtcaata ctgacttact ggatgaactg
     3001 ccggttttca cctttttagc ccatcgtagt gcatcgatac tcatacgaac ccctggcaga
     3061 catttgttta tctgcaaagt aatattggta ttgctgacga tacgcgtgct tgaaagcaat
     3121 agctttttct ataagctcgt cagtctcacg ttccacaaca actggatccg caaaaagcag
     3181 cccggactcc accacatcgc catattcttt gtttaacccg gcgatcatgt acgtaatgct
     3241 ttttccgtca gtaatttcac aatacaatct gaaatcgctg atccggatag cctccataat
     3301 tgccggaatc agcgccgtga atttgtcacg cttatctctg gtgtcgatag ctttccagcg
     3361 ttcgaatatc ttcacccggt taacgcccag cgcccgttga tcaacctcgc catcattaaa
     3421 cgtgacgcgt tgaacatcga tgttcgggcg ttctttcaga gcccagaatg cttccgtgat
     3481 taatatcgtc gcctgctcct gtgtcattcc tggtcggcat acacaggtat ccagagcctc
     3541 acaaacctgt tcagtggtga ttttcattgt tcaaccgccc cgcccgcttt gccttacgat
     3601 attcgtcata aactttgggg tcgtactgaa gttccccgcc ggatgcctct tgcaggcgca
     3661 tcgcgcgacc ttcaggaacc agttcccccc atgcagcaac gcttgccagc ctaactcctg
     3721 cgacattggc aagctttgtt ttgctgccaa aaaacgctat agcatcaatt ttcaacatat
     3781 cgaacccctt agattttcct aaggaaacta gatcgtagag aaacctaagt caagaaaaat
     3841 tagaattacc taatatgaaa aacgaaacct tcggtgctcg cctcttatac aggcgaaaaa
     3901 aattaaaact gtctcaggcc gcattaggta agctggtcaa agtggctcac gtaacaattt
     3961 ctcaatggga aagagatgaa acacagccag cggggaagag attattcgca ctgagccagg
     4021 cgcttcagtg ctcgccgact tggcttcttt ttggagatga agataagcaa ccaggcgaac
     4081 cgatcccaga taatcagcca gtcaatctga cagaagatca aaaagagttg cttcaactgt
     4141 tcgacgcact gcctgagtca gagcaaaagg ctctgttgtc agagatgcgt gctcgagttg
     4201 agaatttcaa caaacttttt gaagaactac tcaaagctcg caaaagaagc gcaaataaat
     4261 aacccccctt tttttcgcaa ctctctgtaa taaaaagcac aaactttcaa atacttgtgt
     4321 tttttacatc aagaagctta ggtttttcta cacaaaaagc ttgaccataa ctcttaggct
     4381 attctaaatt ctactcatca agacaccgca cggtgttctc agcaaacagt tccgctaccc
     4441 ggcgttaagg ggaagcagag gatttctcag tgggcgaagt caaacatcag aatggaaggc
     4501 gtccagggat cagcaaagaa acagcgatgg cgctttatat tgatatcagc gccattgccg
     4561 gacaggtaag agttatcaga gcggtaacta agcggtatgc gcctttactt cagaaagtct
     4621 ctggtgagtg caccgaagat attgtcaacg atttcgtcat cgaactgcga ggactcatct
     4681 tcagttacaa ggtgaccaca atttttgcag atggctcccg cgaaactgtc agagccctgc
     4741 ggcttaaagg atgtgtcaaa gacttagcca ccacattctg ggcaagaaaa cttgattgta
     4801 ttcataacca atttcctctc gagtaacaga cccctcagag gataccacct cgcctgacgt
     4861 ggttaaaaag caggcaacgc taaccacaag gagccgacat gcagaaacga gaccccgtca
     4921 tcatcgcgcc agactatacc gatgatgaac tttatgagtg gatgcaccag aaaattaagg
     4981 cggtgcagga cctgaaatgg gccaatgaag ccagggctaa gcaggctgaa aatctgtccg
     5041 ctctggagca ggatatcacc aatctggaaa aagcagcggc attaagcatt gccagaatga
     5101 ttacataccc acgttaatag ctaaccaacg aggctaataa tggaatttaa agatttacca
     5161 acgccattac aggaaatggc atcgaatata gttcgttcac aactggctac tcttgacctt
     5221 agtaccgcag aaaaagaaac catcgataat atggttcgta atgtgcgcaa tgctttttct
     5281 gggctatatg gttctgataa tcaaaagcag gaaagcgatg ttaataaacg ggtaatttct
     5341 gtttgcgtga atggccatgt tctttcatca atcaaaacag aaacggcgac agtcttcgat
     5401 tgcctttgca ttgtacagag ccttgttgat gccctgtttc gttcagtgaa tttagaaaat
     5461 gatgcaaatc tgcgagggcg cataatagca catccatatg cacatacttt aggctctgtg
     5521 gatatcaaag atcccacaaa tctttaatga aatagttaac gcgaattgta cttgctcttt
     5581 cggttgcttt cagaatacgc gttgaaactg ctggcggtaa tttggtattc catttattaa
     5641 aatcatgccc gggaaagtac tcttcgaaaa tacttttaac tgcagactcg cctattgaaa
     5701 tgctgcttac catgcgattt tgataaaggc atttagcaat aagagttgat tttaacattc
     5761 accctcctga gggttggtaa ttaaggagtt ctccacgggt gaagtggagt gcgtgcgccg
     5821 gacacgggtg aacatccggc actgacagtt tactgaaagg atatttctct gaaaagtcag
     5881 accataacgc gaaagcgcac ggcgaggtag ctggttcata gatagcctgt cgttaaattt
     5941 tcgtcgaccg tgcgcttccg gttgtggcaa tccgcgaaat ggcgcggcgg taagtatgga
     6001 ggggttattc cttccccgtt gaggacaccg ggttgtcagg ttgaccatac gcttaagtga
     6061 caaccccgct gcaacgccct ctgttatcaa ttttctggtg acgtttggcg gtatcagttt
     6121 tactccgtga ctgctctgcc gcccttttta aagtgaattt tgtgatgtgg tgaatgcggc
     6181 tgagcgcacg cggaacagtt aaaaccaaaa acagtgttat gggtggattc tctgtatccg
     6241 gcgttaattg ttaactggtt aacgtcacct ggaggcacca ggcaccgcat cacaaaattc
     6301 attgttgagg acgcgataat ggaaacgtta ttaccaaacg ttaatacgtc tgaaggttgt
     6361 tttgatattg gtgttctgct cagtaaccgg gagtttacgg aagatgccat taagatgaga
     6421 aaatatgaac cttatcttct caatgataat tccatacttt ccagaattgc ccttcttgaa
     6481 cttggtatta tcggagaaca gcagtgactt cagcatttgc actggtgatg accgtttttc
     6541 ttataacggg tgagccacaa aatgtgatta ccggaattta tgacagtaag tcatcctgca
     6601 ttcaggtaag ggacgaacaa aaaatccccg gtgaatgcct cccgttaaaa aaagtatcgc
     6661 tgaacctgaa taacgaaata ccggctggat aacccgccag ccatattaac gccataccaa
     6721 cggattaaaa atgccagcaa tggcagggat tcgttcaccc tgaaatctgt aatgaggtta
     6781 aaacaaaatg agtaaagtct ttatttgcgc cgccattccg gacgaacagg caataaagga
     6841 agaaggtgcc gtcgctgtag ccactgccat tgaagccggt gatgaacgtc gcgcccgcgc
     6901 aaaatttcac tggcaattcc tggaacatta tccggctgct caggactgcg cttataaatt
     6961 tcttgtctgc gaggataaac ccggtatacc ccgccctgcc ctcgattcct gggatgctga
     7021 atatatgcag gaaaaccgct gggatgagga atccgcttcc tttattccgg tcgaaccaga
     7081 atccgatccg atgaacgtca attttgacaa gctgtccctt gaagtacaga acgcggtcct
     7141 ggttaagttc ggtacatgtg aaaacatcac cgttgatatg gcgattgacg cgcaggaatt
     7201 actgcaggaa gacgttgcta cctttgacgg gcatatcgtt gaagcactga tgaaaacgcc
     7261 tgaaattaac gctatgtatc cggaacgcaa actgttcgct atcggatggg ttaaacacaa
     7321 atgtaatccg ggtgccaaat ggcccgaaat tcaggctgaa ttacgtaact ggaaaaaacg
     7381 gcaggacgca gagcgcaaag agactggaaa atacacgtct gttgttgatc tcgccagcgc
     7441 cagagtcaat caacagaaca ctgaaaactc agcaggaaaa atcaacccag tcactgccgc
     7501 catttgtcgc gaatacaagc agacatggaa aacgctggat ga
//
LOCUS       DAZFWL020000188         7539 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFWL020000188 DAZFWL020000000
VERSION     DAZFWL020000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.188"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(113..817)
                     /locus_tag="ACHKNP_RS22585"
                     /old_locus_tag="pgaptmp_004517"
     CDS             complement(113..817)
                     /locus_tag="ACHKNP_RS22585"
                     /old_locus_tag="pgaptmp_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU4706548.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(954..1406)
                     /gene="moaE"
                     /locus_tag="ACHKNP_RS22590"
                     /old_locus_tag="pgaptmp_004518"
     CDS             complement(954..1406)
                     /gene="moaE"
                     /locus_tag="ACHKNP_RS22590"
                     /old_locus_tag="pgaptmp_004518"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HGU4706549.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1408..1653)
                     /gene="moaD"
                     /locus_tag="ACHKNP_RS22595"
                     /old_locus_tag="pgaptmp_004519"
     CDS             complement(1408..1653)
                     /gene="moaD"
                     /locus_tag="ACHKNP_RS22595"
                     /old_locus_tag="pgaptmp_004519"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HGU4706550.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1646..2131)
                     /gene="moaC"
                     /locus_tag="ACHKNP_RS22600"
                     /old_locus_tag="pgaptmp_004520"
     CDS             complement(1646..2131)
                     /gene="moaC"
                     /locus_tag="ACHKNP_RS22600"
                     /old_locus_tag="pgaptmp_004520"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HGU4706551.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVTALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2134..2646)
                     /gene="moaB"
                     /locus_tag="ACHKNP_RS22605"
                     /old_locus_tag="pgaptmp_004521"
     CDS             complement(2134..2646)
                     /gene="moaB"
                     /locus_tag="ACHKNP_RS22605"
                     /old_locus_tag="pgaptmp_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HGU4706552.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2668..3657)
                     /gene="moaA"
                     /locus_tag="ACHKNP_RS22610"
                     /old_locus_tag="pgaptmp_004522"
     CDS             complement(2668..3657)
                     /gene="moaA"
                     /locus_tag="ACHKNP_RS22610"
                     /old_locus_tag="pgaptmp_004522"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HGU4706553.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3645..3789)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4054..4962
                     /gene="yvcK"
                     /locus_tag="ACHKNP_RS22615"
                     /old_locus_tag="pgaptmp_004523"
     CDS             4054..4962
                     /gene="yvcK"
                     /locus_tag="ACHKNP_RS22615"
                     /old_locus_tag="pgaptmp_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HGU4706554.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5154..7175)
                     /gene="uvrB"
                     /locus_tag="ACHKNP_RS22620"
                     /old_locus_tag="pgaptmp_004524"
     CDS             complement(5154..7175)
                     /gene="uvrB"
                     /locus_tag="ACHKNP_RS22620"
                     /old_locus_tag="pgaptmp_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGU4706555.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1815 a         2001 c         1886 g         1837 t
ORIGIN      
        1 aaacgcttcc gctcgccata aggcgtattt tatacttggt acttgttaag tgtcgttttt
       61 agtaagaaaa aaaatcccga cacatggccg ggatattgat ggtgaaaaag aattaacggc
      121 ggttgccaaa aattcgcagc aacatcagga acaggttgat gaagtccaga tacaaggtta
      181 acgcgccaag aatggaatat ttgcgcaggt tcgacgtgtc gcgggtatca atctgctcac
      241 ccatattttt caatttctga gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga
      301 tgtaggtaac tgcccacatc aatgcttcgc ttttcagcca gaagttgacc agcgatgcca
      361 gcacaatgcc gattaacgcc ataaacagca tattgccaaa gccacttaaa tcgcgcttcg
      421 tggtgtaacc gtacaggctc attgcgccga acatcccggc agtaacgacg aaagtactgg
      481 cgatagaagc agcggtatag acaatgaata tactggaaag cgtaagaccc gtcagcgccg
      541 aataaagcat aaagagcatc gtcgttacac ctgcgctcag cttttgaatc atcgctgata
      601 acacaataac caatgctaat tgcgcgatga tcagaccgat taaaaagaca cggttagtaa
      661 acaacagctc catcacggcc gcggaattag ccgcatacca ggcaacaaat gcggtcagca
      721 acaagccaac ggtcatccag ccatagactt gcgccatata agtttgcaag ccagcccggg
      781 gttgtacgat tgaatcagaa cgtgggaatc tgtccatgac gatctcctga agatataagg
      841 aatatcttaa ggatactgca aaatgatgag gctgtgcatc gacgcagcgt aaacgcatgt
      901 actgagcggt gaaattgccg gacgcagcgg tgccttatcc ggctaacaaa aaactaccag
      961 cgttttgccg cctgctgatc gctctcccga gcttcaaccc agcggtcgcc ttccggcgtg
     1021 gcttcgcgct tccagaacgg tgcgcgggtt ttgagataat ccataataaa ctgcccggct
     1081 tcaaacgcac tgctgcgatg cgcactggtg acaccgacaa aaacgatttc atcgcccggc
     1141 cataattccc cgatgcggtg aatcacagtg acgcgcccca gcggccagcg gttacgcgct
     1201 tcatcaacaa tttctgccag tgctttttca gtcatccccg gatagtgttc gagggttaat
     1261 gctttgacgc tgtcgcccag gttatggttg cgcaccttac cagtaaaggt gactaccgca
     1321 ccgtcttcgt cacgctccgc cagccacggg tactcttctc ctacgctgaa cggctgcgga
     1381 ccaacaacaa ttttggtttc tgccatctta acctccggtt accggcggga agaaagctac
     1441 ttcgtcgccg tcagtcagcg gatggtcaaa actcaccagc gtctggttga cggcagccag
     1501 taatttgcca tcttccagcg ccagcgccca gcgatcgctc tgcgcagcca tgtgctggcg
     1561 taacgcttca acagttggga aatccgcagc cacttcggtt gcatctgttc ccaccaactc
     1621 gcgcacctgg gcgaaaaaaa gaactttaat catccgcttc caccttaaag tcacccgact
     1681 tgccgccgct cttcgccagc aaacgtaccg gaccaatcac catatctttt tgcaccgctt
     1741 tgcacatgtc ataaatggtc agcgccgtca cggaggccgc ggttaatgct tccatttcga
     1801 caccggtttt cccggtcagg cggcataagg tttctatacg cacccgattg tgctccggct
     1861 cggcctgtaa attgacttca actttgctga gcatcagcgg atgacagagc gggatcagat
     1921 cccaggtgcg ttttgccgcc tgaataccgg caatacgcgc agtggcaaat acgtcgcctt
     1981 tgtggtggcg accatcaata atcatcgcca gcgtctcgct gcgcatggtg acaaaggctt
     2041 ccgcccgcgc ttcacgcacg gtttccgctt tggcggagac atccaccatg tgcgcttcgc
     2101 cagcggcgtt gatatgggtc agttgcgaca tacttatttc ttcaaatgtg gatggaaatt
     2161 acacggacgc gtacgggcat ccagctgcgg cgcgatgata ttttcccatg cggtacggca
     2221 cgctttggtc gaacccggca tggcgaaaat cagcgttttg ttggcgacgc ccgctaccgc
     2281 acgagattgc aacgtggaag tgccaatctc ttcaaacgac aacatacgga acacttcacc
     2341 aaaaccttca atttcacggt cgaacaacgg cagcaatgct tcgggagcct gatcaccttc
     2401 cgtcaggcca gtaccacccg taatcaatac cacttgtaca tcgtcgctgg cgatccacgc
     2461 agatacctga gcgcgaatag cgtagcggtt ttctttcaca atggctttat cgacaacgtg
     2521 atggcccgct tcttgcgccg aatcgcgcag atagtgaccg gaggtatcgt cttcttcacc
     2581 gcgacgatta gaaaccgtaa gaatagcaat acgggtcggg ataaattcag tgcttacctg
     2641 actcatctga tctctccttt tgacgtttta gccgccaatg tacgataagt tttgcgtaat
     2701 accggtgttg ttttgatgca ggaaatgggt ctgtttcttc tcccgcagcg ccgctgaaat
     2761 acgcgcttcc agcgcctgtt gctgggtatc gtcttccagc agatcgcgca ggttaacgcc
     2821 gccttcacca aacaggcaga gatggagttt accaatggag gaaacgcgca ggcggttgca
     2881 agtggcgcag aagtcttttt catacggcat gataaggcca atctctccgg cgtaatctgg
     2941 atggcaaaag acttgcgcgg gaccgtcgct gcgttgacgt aattggtgga tccagccgcg
     3001 acgcagtagc tcgtcacgca gaacctgacc agagatgtga tgcttacgga agagctcgct
     3061 gccctcgccc gtttccatca gttcgatgaa acgcagctgg atagggcgat gctggatcca
     3121 gttcagaaag gtgtcgagct ggtgatgatt aacatcacgc atcagcacgg tattgacttt
     3181 gaccttctca aaaccggcct caaatgcagc atcaatcccc gccatgacct ggttgaattt
     3241 atcctgcccg gtaatagcgt gaaactggcg ggcgtccaga ctgtcgacac tgacgttaat
     3301 gccagtaagt cccgcatcgc gccagttcgc cacatcgcgt tccagacggt aaccattggt
     3361 ggtgaccgca atctggcgga tagcgtcgtt ttcccgcaca gcggcgatga tatcggtaaa
     3421 gtcgcggcgt aaagacggct caccgcctgt caggcgcact ttttcggtgc ccagactggc
     3481 gaaggcgcgc gtaacccggc gaatttcatc gacggtaaga aagcctttat tggtgacgcc
     3541 gctcggtttg tagccatccg gcaggcagta ggtgcaacga aagttacaca catcggtaat
     3601 cgacaggcgc aagtagtaaa acttacgcgc aaatgcatca gtcagttgtg aagccatgta
     3661 cacctttcca gatacgggag gcgaagtcat ttcttccttc gccctggtgg caatctttac
     3721 gagcattgtc acggccaaag caccgtatca gttgacccag gtgcagaggc tagagtgttt
     3781 agtggttatg ccgatactag cgtgtaaatg agatttttac catccacact ttcgctatgt
     3841 aatcatgtat atagcgtcat gatcgtgcta tttcgctaca gaataaggga aaatggcggt
     3901 tttactttga tatacatcat cagggaggca tagcgtgcat cgtcctttag aggtagaaga
     3961 aaatagtttg tatcctcaat attggcaggt taattgctgt ttccccgcaa tttgcgctac
     4021 tgtagcgcgg ataatctgac tccaggaaac tatatgcgca atcgtacgct ggctgatctt
     4081 gatcgtgtcg ttgctctcgg cggagggcat ggactgggac gcgttctctc atcactttcg
     4141 tctttgggtt ctcgtttaac gggtatcgtc accaccaccg ataatggtgg ctcgacgggg
     4201 cgtattcgcc gttcagaagg cggcattgcc tggggcgata tgcgcaactg cctcaaccag
     4261 ctgataacgg aaccgagcgt cgcctccgcg atgtttgaat accgttttgg tggcaatggc
     4321 gaactttccg gtcataatct cggaaacttg atgttaaagg cgctggatca ccttagcgtg
     4381 cggcctctgg aagccatcaa tttaattcgt aatctgctga aagtggatac gcatttgatt
     4441 ccaatgtcag agcatcctgt tgatctgatg gcgattgacg atcaggggca tgaagtttac
     4501 ggcgaggtca atatcgacca gttaactacg ccgattcaag agttattgtt aacgcctaat
     4561 gtacccgcaa cgcgtgaggc ggttcacgct atcaatgaag cggatctcat cattattggg
     4621 cctggcagtt tttataccag cctgatgcca attctgctgc tgaaggaaat cgcccaggca
     4681 ttacgccgca cgccagcgcc gatggtttat atcggcaatc tggggcgtga gttgagttta
     4741 cctgcggcta atttgaagct ggaaagcaag ctggcaatta tggagcagta tgttggtaaa
     4801 aaggtcattg atgcggtcat cgtcgggcca aaagtggatg tctcggcggt gaaagagcgg
     4861 attgtgatcc aggaggtact ggaggccagc gatattccgt atcgtcatga ccgccagttg
     4921 ttacataacg cgctggaaaa ggcgttacag gctttaggtt aacggaaatc cgaaggaaaa
     4981 ttccggcttc ctattgaaga caaagtgcgc gttgtttatg ccggatgcgg cgtgaacgcc
     5041 ttatccggcc tacaaaccgc gcaaattcaa tatattgcgg agaaaatgta ggcctgataa
     5101 gcgtagcgca tcaggctgtt ttccgtttgt catcagtctt cttcgctatc ctgttacgat
     5161 gccgcgataa acagctcacg cagctgatgc aactggtcac gaatttgcgc cgcttcttcg
     5221 aactccagat tctgcgcgtg ttgcatcatc aacccttcca gctcatggat tttctgctgc
     5281 aacgctttag gcgacatatc catcggcaca ttatccggct caacaatcgg tcgcgatttt
     5341 cctctgccct tcgctttggt tttagcaatg ttctgcccca acgccaggat atcgaccact
     5401 ttcttgttca agccttgcgg cgtaatgccg tgttcctcgt tgtacttctg ctgtttctcg
     5461 cggcgacgtt cggtttcgcc aatcgctttc gccattgatg gggtgatctt atcgccgtag
     5521 agaatcgctt taccgttaac gttacgtgcc gcacgaccaa tggtctggat caacgaacgt
     5581 tcggaacgca ggaagccttc tttgtcagcg tcgaggatcg ccaccagcga aacttccggc
     5641 atatccagac cttcacgcag taagttgatc cctaccaata cgtcgaactc acccagacgc
     5701 aagtcgcgga taatctccat acgctcgacg gtgtcgatat ctgagtgaag ataacgcacg
     5761 cgctcaccgt gttcttcgag atattcggta agatcttccg ccattcgctt ggtcagtgtg
     5821 gtgaccagta cgcgttcgtt aattgccgct cgctgacgaa tctccgaaag aagatcatca
     5881 acctgtgtcg ccaccggccg cacttcgata atcgggtcaa gcaatccggt tggacgcacc
     5941 acctgatcca ccacatcgcc gccggatttt tccagctcgt aattacccgg cgtcgccgaa
     6001 acatagatgg tttgcggcgc taatgcttcg aactcttcaa acttaagcgg acggttatcc
     6061 agcgctgatg gcaggcggaa gccgtactcc accagtgtct ctttacgcgc ccggtcaccg
     6121 cgatacatgc cgccaatttg tggaatggtg acgtgagatt catcgaccac cagcagccca
     6181 tcagcaggga ggtaatcaaa cagcgtcggc ggtggctcac ccggtccacg accggagagg
     6241 aagcgcgagt agttttcaat ccccgaacag tagcccagct cgttcatcat ctccagatca
     6301 aactgggtac gctgggtcag ccgctgctct tccagcagtt tgttgttttc caacagcact
     6361 ttgcgtctgg cggccagctc ttctttgatc tcctccatcg cctgtacgat gcgctcgcgc
     6421 ggtgtgacgt agtgcgtttt cgggtagatg gtaaaacgtg gaatagtgga aacaatctgc
     6481 ccggtcagcg ggtcaaataa cgacaatcgt tccacttcct cgtcaaacag ttccacgcga
     6541 agtgcaatgt catccgattc tgccgggaag atatctatca cctcgccacg aacgcggaaa
     6601 gtaccacgct ggaatgcttg atcattacga gcgtattgca gctccgccag tcggcgcaga
     6661 atcgcgcgct gatcgataat cataccgacc gtgagatgga gcatcatctt gagatataaa
     6721 tcaggatcgc ccagaccata aatcgcggaa acagacgcca ccacaaccac atcacgccgc
     6781 tccagcatcg ctttggtggc ggacaaacgc atctgctcaa tatgttcgtt aaccgaggca
     6841 tctttctcaa tgaaagtgtc ggaactcggt acataggctt ccggctgata gtagtcgtag
     6901 taggaaacga aatattccac cgcgttttcc gggaagaact ctttcatttc gccatacagc
     6961 tgggccgcca gcgttttgtt gggcgcaagt accatggttg ggcgctgaag gtcagcaatg
     7021 acattggcaa tggtgaatgt tttcccggag ccagtcacgc caagtaacgt ctggtgcgcc
     7081 aggccatctt ccagcccctc ttcgagacgt cgaatcgcct ctggctgatc gccagaaggt
     7141 ttaaaagcgg aattcagttt gaacggttta ctcatgagtc gctacctgaa ggagttgggc
     7201 gggcaggtat gtaattttac tcgtcgtact taattatgcc aacaaattat actggataaa
     7261 aaaacagttc atcaccataa tatttctgtt acagcgtaaa ctccgctgtc aatcatgagc
     7321 aaaatttact ctgtggcgag ataaaactcc ggccttaccg ggttatcccc aaagcaacgg
     7381 cttttttaac atttgtcaag atgagtgatg acagttttat ggcaagagat gcctgttcag
     7441 tgactgccat tgcatttatc taaccagtta aaaaataaaa gatatttctt tgagccgtct
     7501 ttaacgccaa ttcgcgtgca agccgcgtat tctctcgct
//
LOCUS       DAZFWL020000189         7509 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFWL020000189 DAZFWL020000000
VERSION     DAZFWL020000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.189"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..215
                     /gene="ecpR"
                     /locus_tag="ACHKNP_RS22625"
                     /old_locus_tag="pgaptmp_004525"
                     /gene_synonym="matA"
     CDS             <1..215
                     /gene="ecpR"
                     /locus_tag="ACHKNP_RS22625"
                     /old_locus_tag="pgaptmp_004525"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HGU4706556.1"
                     /translation="LAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIEN
                     CSVKTVYTHRRNAEAKLYSKIYKLVQ"
     gene            289..876
                     /gene="ecpA"
                     /locus_tag="ACHKNP_RS22630"
                     /old_locus_tag="pgaptmp_004526"
                     /gene_synonym="matB"
     CDS             289..876
                     /gene="ecpA"
                     /locus_tag="ACHKNP_RS22630"
                     /old_locus_tag="pgaptmp_004526"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HGU4706557.1"
                     /translation="MKKKVLAIALVTVFTGIGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            934..1602
                     /gene="ecpB"
                     /locus_tag="ACHKNP_RS22635"
                     /old_locus_tag="pgaptmp_004527"
                     /gene_synonym="yagY"
     CDS             934..1602
                     /gene="ecpB"
                     /locus_tag="ACHKNP_RS22635"
                     /old_locus_tag="pgaptmp_004527"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HGU4706558.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1628..4153
                     /gene="ecpC"
                     /locus_tag="ACHKNP_RS22640"
                     /old_locus_tag="pgaptmp_004528"
                     /gene_synonym="yagX"
     CDS             1628..4153
                     /gene="ecpC"
                     /locus_tag="ACHKNP_RS22640"
                     /old_locus_tag="pgaptmp_004528"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HGU4706559.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAEVTQTGEENES"
     gene            4143..5786
                     /gene="ecpD"
                     /locus_tag="ACHKNP_RS22645"
                     /old_locus_tag="pgaptmp_004529"
     CDS             4143..5786
                     /gene="ecpD"
                     /locus_tag="ACHKNP_RS22645"
                     /old_locus_tag="pgaptmp_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HGU4706560.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            5755..6465
                     /gene="ecpE"
                     /locus_tag="ACHKNP_RS22650"
                     /old_locus_tag="pgaptmp_004530"
     CDS             5755..6465
                     /gene="ecpE"
                     /locus_tag="ACHKNP_RS22650"
                     /old_locus_tag="pgaptmp_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HGU4706561.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6778..7107
                     /gene="ykgJ"
                     /locus_tag="ACHKNP_RS22655"
                     /old_locus_tag="pgaptmp_004531"
     CDS             6778..7107
                     /gene="ykgJ"
                     /locus_tag="ACHKNP_RS22655"
                     /old_locus_tag="pgaptmp_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU4706562.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7355..>7509)
                     /locus_tag="ACHKNP_RS22660"
                     /old_locus_tag="pgaptmp_004532"
     CDS             complement(7355..>7509)
                     /locus_tag="ACHKNP_RS22660"
                     /old_locus_tag="pgaptmp_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HGU4706563.1"
                     /translation="PPLFDLPWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEI
                     PLGSNR"
BASE COUNT         1927 a         1955 c         1948 g         1679 t
ORIGIN      
        1 aactggccta tgtgattaat ggcaggttcc tgagaaaaga tattaagaaa gataaaatca
       61 cggaccggga aatggaaatt atccgcatga cggcccaggg aatgcaacct aaatcgattg
      121 ccagaattga aaattgtagt gtgaagacag tgtataccca tcggcgtaat gctgaggcca
      181 agctgtactc aaaaatatat aagttggttc agtaaactcc aggctagtta gttttaaaaa
      241 tgactcactg ggacatcacg tcctcaattc aactcgggaa gaaatgcaat gaaaaaaaag
      301 gttctggcaa tagctctggt aacggtgttt accggtatag gtgtggcgca ggctgctgac
      361 gtaacagctc aggctgtagc gacctggtca gcaacagcca aaaaagacac caccagtaag
      421 ctggttgtga cgccactcgg tagcctagcg ttccagtatg ccgaaggcat taaaggtttt
      481 aactcacaga aaggtctatt tgacgtggct atcgagggtg actcaacggc taccgccttt
      541 aaactgacct cacgtcttat caccaacaca ttaacccagt tggatacctc aggttccaca
      601 ctgaatgtgg gcgtggatta taacggcgcg gcagtcgaaa aaactggcga taccgtgatg
      661 atcgataccg ccaacggcgt actgggcggc aaccttagcc cgctggcaaa cggttacaat
      721 gccagcaatc gtaccaccgc acaggatggt ttcactttct ccatcatcag cggcaccacc
      781 aatggtacca ccgcagtaac cgattacagc actctaccgg aaggcatctg gagcggcgac
      841 gttagcgtac agttcgacgc gacctggacc agttaatctc tctgatgtac cagcaggggt
      901 agcccccctg ctttattcct ggacggaatt gttatgaaaa agcaccttct gcctctcgct
      961 ctgctgtttt ccggaatatc tccggcccag gcgctggatg tcggcgatat atcatcgttt
     1021 atgaacagtg acagcagcac gctgagcaaa acgatcaaaa acagtaccga cagtggtcgc
     1081 cttatcaata tccgtctcga acggctctct tcaccgcttg acgacgggca ggttatctca
     1141 atggacaagc cggatgagtt gctactcact cccgccagct tgctgctacc cgcccaagcc
     1201 agcgaagtga tccgcttctt ctataaggga cccgcagatg aaaaagagcg ctactaccgc
     1261 attgtctggt ttgatcaggc cctcagtgat gcgcagcgcg ataatgccaa ccgcagcgct
     1321 gtggccactg cttccgcccg catcggcacc attctggtcg tcgcccctcg tcaggcgaac
     1381 taccactttc agtacgccaa cggctccctg acaaatacag gaaatgcgac gctgcggatc
     1441 ctcgcttacg gcccctgcct gaaagccgcc aatggtaagg agtgtaaaga gaattactac
     1501 ctgatgccag gcaagtcgcg tcgttttacc cgcgtggaca cggcggataa caaaggacgg
     1561 gttgcacttt ggcagggtga taagttcatt cccgtgaaat agatagctgt gcagatggat
     1621 aacgacaatg cctttacgac ggttctcccc aggactgaaa gcccagtttg ccttcggcat
     1681 ggtctttttg ttcgttcaac ccgatgccag cgctgctgac ataagtgcgc agcaaatagg
     1741 tggggtgatt attccgcagg ccttcagtca ggcgcttcag gacggcatga gcgtcccgct
     1801 ctatattcat ctcgccggta gccagggtcg ccaggacgat cagcgaatcg gcagcgcttt
     1861 tatctggctg gatgatggac agctacgcat ccggaaaata cagctggaag agagtgaaga
     1921 taacgccagt gtcagcgaac aaactcgaca gcagctgatg gctctggcga acgccccgtt
     1981 caatgaggcc cttaccatcc ccctgactga caacgcgcag ctggatctca gcttgcgcca
     2041 actgctgctg cagctggtgg tcaagcgcga agcgctgggc accgtactac gctcacgtag
     2101 cgaagacatc gggcagtcca gtgttaacac cctcagcagt aatctgagct ataacttggg
     2161 cgtctataac aaccagttgc gtaacggcgg gagcaacaca tccagctatc tgtcgctgaa
     2221 taacgttact gcgctgcgcg aacatcatgt ggtgctcgac ggctcgctgt acgggatcgg
     2281 tagcggtcaa caggacagtg aattatataa agcgatgtat gaacgcgatt ttgccggtca
     2341 ccgatttgcc ggtggaatgc tcgacacctg gaacttgcag tccttagggc cgatgaccgc
     2401 catttcagca gggaagattt acggcctttc ctggggaaac caggccagct ccaccatctt
     2461 cgacagcagc cagtcagcca cgccagtgat cgccttttta ccggcggcgg gcgaagtaca
     2521 tctcacccgt gatgggcggc tactaagcgt tcagaacttc actatgggca atcatgaagt
     2581 ggatacccgg ggtctaccgt acgggattta cgatgtggaa gttgaggtga tcgttaacgg
     2641 tcgcgtgatc agcaaacgcc cccagcgggt caataagctg tttagccggg ggcgcggcgt
     2701 cggtgcacca ctggcgtggc aggtatgggg cggtagcttt catatggatc gctggtcgga
     2761 aaacgggaaa aagacgcgac cagctaaaga gagttggcta gcaggtgcct cgacctccgg
     2821 ctcattgagt acgcttagct gggcggcaac gggatatgga tacgataatc aggcggtggg
     2881 tgaaacccgt ctgacgctgc cgcttggggg ggcgatcaac gttaacctgc aaaatatgct
     2941 ggccagtgac agctcatgga gcagcatcgg cagcatcagc gccactctac cgggaggctt
     3001 tagttcgctg tgggttaacc aggaaaaaac ccgcattggc aatcaattgc gacgtagcga
     3061 tgccgacaac cgtgcaatcg gcggcacact caacctgaac tcactgtggt cgaagctggg
     3121 cacattcagc atcagctaca atgatgaccg ccgttacaac agccattatt acacggcaga
     3181 ttactatcaa aatgtctaca gcggtacctt tggttcgctt ggcctgcggg ccggtattca
     3241 gcgctataac aacggcgaca gcaacgccaa tacagggaaa tatatcgctc tcgatctctc
     3301 gctaccactg ggcaactggt ttagcgcagg gatgactcat caaaacggct acaccatggc
     3361 aaacctgtca gcacgcaaac agtttgatga aggaaccatt cgcactgttg gtgccaatct
     3421 gtcacgtgcc atctccggcg ataccggtga tgacaaaacc ctcagcggtg gggcgtatgc
     3481 acagttcgac gctcgttacg ccagcggaac gctgaacgtc aatagcgcgg cggacggcta
     3541 catcaatacc aacttgaccg ccaatggcag cgtcggctgg cagggtaaaa acatcgctgc
     3601 cagcgggcgg actgatggca acgctggggt gatattcaac accgggctgg aggacgacgg
     3661 tcagatcagc gccaaaatca acgggcggat tttcccgctt aacggcaagc gtaactatct
     3721 cccgctctct ccctatggaa gatatgaggt ggagttacag aacagcaaaa actcactcga
     3781 cagctacgat atcgtcagcg gccgcaaaag ccatctgact ctctatccag gcaatgtcgc
     3841 tgtcattgag ccagaggtga agcagatggt taccgtctcc ggtcgtatcc gtgcggaaga
     3901 cggcacactg ctggctaacg cacggattaa caaccatatc ggccgaaccc gaaccgatga
     3961 aaacggcgag tttgtcatgg acgtggataa gaaatatccc actatcgatt ttcgctacag
     4021 tggcaataaa acctgcgaag tggcactgga actcaaccag gcgcgcggtg ccgtctgggt
     4081 cggtgatgtg gtctgcagcg gcctctcatc gtgggcggag gtgacgcaga caggagaaga
     4141 gaatgagagt taacctacta ataacgatga taattttcgc gctaatctgg ccagtaactg
     4201 agctcagagc ggcagtgagc aaaacaacct gggcggatgc accggcacgc gagtttgtgt
     4261 ttgtcgaaaa caactcagac gacaactttt tcgtcactcc tggcggggcg ctggatccgc
     4321 gcctgaccgg tgccaaccgc tggaccggtt taaaatacac tggttcagga accatctatc
     4381 agcaaagcct cggctacatt gataacggtt acaacaccgg cctttatacc aactggaagt
     4441 ttgatatgtg gctggaaaat tcaccagttt catctccttt aactggcttg cgctgcatca
     4501 actggtacgc tgggtgtaat atgaccacca gtcttatcct gccgcaaacc accgacgcca
     4561 gtggatttta tggcgcgacg gtgaccagcg gcggcgcgaa gtggatgcac ggcatgttgt
     4621 cagacgcgtt ttaccagtat ctgcaacaaa tgcccgtcgg cagcagcttt acaatgacca
     4681 tcaatgcctg ccagacctct gtgaactatg acgccagcag cggcgcacgc tgtaaggatc
     4741 aggcctccgg caactggtat gttcgcaacg tcacccatac gaaagcagca aatctgcggt
     4801 taataaatac ccactcgctg gcggaggtat ttatcaacag cgacggagta ccgactctgg
     4861 gcgaagggaa cgccgactgc cggacacaga ccatcggcag cctttcagga ttaagttgta
     4921 agatggtcaa ctataccctg caaacaaacg gactcagcaa cacctcaatc catatattcc
     4981 cggcgatcgc caactcgtcg ttagcctcgg ccgtcggggc gtacgatatg cagttcagtc
     5041 tgaatggcag ttcatggaaa ccggtgagca ataccgccta ttactacacc ttcaacgaga
     5101 tgaagagcgc agactcgatc tatgttttct tctcgagcaa cttctttaag cagatggtga
     5161 acctcgggat cagcgatatc aacaccaaag atctattcaa ctttcgcttt cagaacacca
     5221 catcaccgga gtctggctgg tatgaatttt ctacctccaa cacgctgatt atcaaacccc
     5281 gtgatttcag catcagtatt atctccgatg aatatactca gacaccgtcg cgggagggat
     5341 atgttggcag cggcgagtcg gcactcgatt tcggctatat cgtaaccacc agcggtaaaa
     5401 cagctgccga cgaagtgctg atcaaggtga ccggacccgc gcaggtgatt ggcgggcgct
     5461 cctattgtgt cttcagctcc gatgacggta aggcgaaagt accgttcccg gcgacacttt
     5521 cctttattac ccgcaacgga gctacaaaaa cctacgatgc cgggtgcgat gatagctggc
     5581 gggatatgac cgatgcgctg tggttgacca caccgtggac tgatatctct ggcgaagtgg
     5641 ggcagatgga taagaccaca gtcaaatttt cgattccaat ggataacgcc atttcgctgc
     5701 gtacggtaga tgataacggc tggtttggcg aagtcagcgc ttcaggagag attcatgttc
     5761 aggcgacgtg gcgtaacatt aactaaggcc ctgctgacag cggtctgtat gctggcggca
     5821 cctttgacac aggcgatttc ggtcggcaat ctgacatttt cgctgccgtc cgagactgac
     5881 tttgtcagca aacgtgtagt gaataacaac aaaagcgcac ggatataccg tattgccatc
     5941 agtgctattg atagcccggg cagcagtgaa ttgcgcaccc gaccggtgga tggtgaactg
     6001 ctttttgccc cccgccaact ggcgttgcag gctggtgaga gcgagtattt taaattttac
     6061 tatcatggtc cacgggataa ccgcgagcgc tactaccggg tctcatttcg cgaagtcccc
     6121 actcgtaacc agacaagacg tagcccaacc ggcggcgagg tcagcacgga gccggtggtg
     6181 gtgatggata ccattctggt agtacgacca cgtcaggttc agtttaaatg gtccttcgac
     6241 aaggtgacag gaacggtaag taacactggc aacacatggt ttaagctact gattaagcca
     6301 ggatgtgatt cgaccgaaga ggaaggcgat gcctggtatc tacgtccggg agacgtggtt
     6361 catcagcctg agttacgtca gccggggaat cattatctgg tctataacga caaattcatt
     6421 aagattagcg attcctgtcc ggctaagccc ccttcggcgg actaagactt atccatcggt
     6481 cgagggaaga attccacctc agagctccaa aaatcgttta aatgatgtgg aagcgatcgt
     6541 caatgtcacc tgtaacgatg ggctttctca tatgcacacc atagtgttca ggagacacgg
     6601 aaaggtatca ggcagcctgc taatgagtgg tttaatcacg aattagtacg taaaatcggt
     6661 aacggctgga aatcattcaa tactcacact atcgaaagtt ccccagccaa ccgcggtacg
     6721 ttcttacata cgatgtaccg ctgttctctt tacgattttt agctgtactg gtgaattatg
     6781 agcaatctga atccatgcat gacgtgtggt gcctgttgtg catttttccg cgtctctttt
     6841 tactgggccg aagccgacga tgctggcggt actattcccg ccaggctcac tgaacaaata
     6901 tccccttttc accgatgcat gagcggtacc aatcagaaaa acccccgatg tattgccctt
     6961 gcaggaaccc cgggcaaaaa tgcctgctgc acgatatata aaaatcgatc gtccacatgc
     7021 agagaatttg ccatgtctgg tgagaacgga gtcgtcaatg aggcttgcaa tcgtgcaagg
     7081 gctaaatacg ggctgacacc gttataaaca tacaataatt aattgcactg ccccgccagc
     7141 gataatagcg gggcttcgtt tttcaggggt aacaaaaccc gatacttctt ctatttgcca
     7201 gcaacaatgc ctctcttctg tagcgttctt cggatctaca tcatcctgaa gtagcgcgat
     7261 atactcactg actatctgaa tcagaatata aaaagcaatg tttttaacct ataaaaatgg
     7321 cgctgtattt gcgccatttt tatcattcaa tgcattatct gtttgagcct aaagggatct
     7381 cagggtctgg ctcatgagta attctgtttc gtaaatctct gcgaataatt tcaatagacc
     7441 agaaccagac taaatgtcca aaaatttcag aaacattctc ataccacggg agatcaaaca
     7501 gaggtggcg
//
LOCUS       DAZFWL020000190         7421 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFWL020000190 DAZFWL020000000
VERSION     DAZFWL020000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.190"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(63..886)
                     /locus_tag="ACHKNP_RS22665"
                     /old_locus_tag="pgaptmp_004533"
                     /pseudo
     CDS             complement(63..886)
                     /locus_tag="ACHKNP_RS22665"
                     /old_locus_tag="pgaptmp_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(940..2658)
                     /gene="proS"
                     /locus_tag="ACHKNP_RS22670"
                     /old_locus_tag="pgaptmp_004534"
     CDS             complement(940..2658)
                     /gene="proS"
                     /locus_tag="ACHKNP_RS22670"
                     /old_locus_tag="pgaptmp_004534"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGU4706564.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2770..3477)
                     /gene="tsaA"
                     /locus_tag="ACHKNP_RS22675"
                     /old_locus_tag="pgaptmp_004535"
     CDS             complement(2770..3477)
                     /gene="tsaA"
                     /locus_tag="ACHKNP_RS22675"
                     /old_locus_tag="pgaptmp_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HGU4706565.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3474..3878)
                     /gene="rcsF"
                     /locus_tag="ACHKNP_RS22680"
                     /old_locus_tag="pgaptmp_004536"
     CDS             complement(3474..3878)
                     /gene="rcsF"
                     /locus_tag="ACHKNP_RS22680"
                     /old_locus_tag="pgaptmp_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HGU4706566.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(3996..4811)
                     /gene="metQ"
                     /locus_tag="ACHKNP_RS22685"
                     /old_locus_tag="pgaptmp_004537"
     CDS             complement(3996..4811)
                     /gene="metQ"
                     /locus_tag="ACHKNP_RS22685"
                     /old_locus_tag="pgaptmp_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HGU4706567.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4851..5504)
                     /locus_tag="ACHKNP_RS22690"
                     /old_locus_tag="pgaptmp_004538"
     CDS             complement(4851..5504)
                     /locus_tag="ACHKNP_RS22690"
                     /old_locus_tag="pgaptmp_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HGU4706568.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5497..6528)
                     /gene="metN"
                     /locus_tag="ACHKNP_RS22695"
                     /old_locus_tag="pgaptmp_004539"
     CDS             complement(5497..6528)
                     /gene="metN"
                     /locus_tag="ACHKNP_RS22695"
                     /old_locus_tag="pgaptmp_004539"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HGU4706569.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6716..7291
                     /gene="gmhB"
                     /locus_tag="ACHKNP_RS22700"
                     /old_locus_tag="pgaptmp_004540"
     CDS             6716..7291
                     /gene="gmhB"
                     /locus_tag="ACHKNP_RS22700"
                     /old_locus_tag="pgaptmp_004540"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HGU4706570.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         1814 a         1925 c         1888 g         1794 t
ORIGIN      
        1 ttttgtaggc cggataaggc gtttacgccg catccggcat tttgtgcacg aggctggctt
       61 tatcactgag tcagcccaac aaagcgaccc gccttgatat aaatcccagg cttcaggtgc
      121 ccaacatact gtaactgcgt ttctggctta aacgcagaga catcaccaat acgcatatgc
      181 atcagatcgg cagggctaat ccagcccgat tgcgccagtg taagtggatg cccggctttg
      241 gcaaaggcat ccgtgacgaa ttccgaacaa aaccacgact ttttgtctcc ttcgcccaca
      301 ctgcttaact gcgctttcgc caggccgctg acgcactttg gcgaaaatcc tcggagaacg
      361 gattcagtga gcacatctgg cgagtcacca taaagggaat aaattcgaca atgccgcgat
      421 agttataacc gctatctttg attttattgg caaacgcggt gatatctgtg gcttgttgcg
      481 gggtaagatc cgggactcgt aagacgaaaa gcttatcact atgcttcatc gcttttttaa
      541 gggaaacaat ctggacgcca gcgcctgtcg cttctgcaac gttattatca cccagaaaga
      601 ttgcaacgtg actcacagag gaagtgctga agacgcggat tccaaatgag gttaccccaa
      661 ggcttgagga gaacagcaaa tcgccgggtt tgagatcagg tgccgttatt tcttttattg
      721 attgttcggt gaaagagctt tgatgctgga atttgacagc ccatgttttt gcctcggcat
      781 ctactgctgt ggctgatggg tctggctggc tgatatcaac ggtacaagcc gaaagtaaaa
      841 gaaagctggg gagaaacagg cggcagtacg cctttggttt atccatttta tacaatccat
      901 gtaaaaaaag ggccctgaaa ttcaggaccc tttctggcat cagcctttaa tctgtttcac
      961 cagatattcg acgatgtcac cagtcttaat taactgtttc tcgccgttgc gacgatattt
     1021 atattcgata tcgtcgttgt cgaggttacg gtcgcccagc acaatagtgt gcggaatacc
     1081 gatcagttcc atatcagcaa acatcacgcc cggacgctct ttgcggtcat ccagcagcac
     1141 ttcgatacct tgtgcacgca gttcgctgta cagtttctca gcaagctctt gtacgcggaa
     1201 ggatttgtgc atgttcatcg gcagaatcgc cacctggaac ggcgcgatag cgtcaggcca
     1261 tacgatgcct cgttcgtcgt agttctgctc aatcgccgca gctaccacac gcgttacccc
     1321 gataccgtag caacccatcg tcaggatttg gttacggcca tcttcaccct gtacggaggc
     1381 tttcagtgct tcggagtact tggtacccag ctggaagatg tgaccaactt cgataccacg
     1441 tttgatcagc agcgtaccct ggccatccgg gcttggatcg ccagccacca cgttacggat
     1501 atctgcaact tccggggtag cgacatcgcg atcccagttg atgccgaagt agtgtttacc
     1561 atcgatgtta gcaccagcag cgaaatcact catcgccgca acggtacggt caatcaccac
     1621 cggaatcggc atgtttaccg gacccagtga acccggaccg gctttaacca cggcacgaat
     1681 ttcttcttcg gtcgcgaaag tcagcgggct tgcaacctgc ggcagttttt ctgctttaac
     1741 ttcgttcagc tcgtgatcac cgcgcaccag cagcgcaacc agcgggaagc tactgccttc
     1801 aaccgcttta accagcagag tcttaaccgt tttctcaatc ggcagattga actgttcaac
     1861 cagttccgcg atggttttcg cgttcggcgt atcaaccagc gtcatttcct gggtagcagc
     1921 agcgcgcggt tctttcggcg cgatagcttc tgccagttca atgttcgctg catagtcaga
     1981 ggtgtcggag aagaccacat cgtcttcacc gctctgcgcc agcacctgga attcgtgaga
     2041 ggcgctgccg ccgatagaac cggtgtcggc ttgtacggcg cggaaatcca gccccatgcg
     2101 gctgaagatt ttgctgtagg ccgcatacat tgcatcgtag gtttcctgca gggattcctg
     2161 agaagtatgg aaagagtaag catctttcat caggaattcg cgggaacgca tgacgccgaa
     2221 acgcggacgc acttcgtcgc ggaacttggt ctggatctga tagaagttca gcggcagctg
     2281 tttgtaagag ctaagctcgt tacgaatcag gtcagtgata acttcttcat gagttgggcc
     2341 gagtacgaac ggacgctcgc cacggtcaac aaaacgcagc agttccggac cgtactgttc
     2401 ccaacgacca ctctcttgcc acaaatcagc tggctgaacc accggcatcg acacctcgat
     2461 cgcaccggcg ttgttcatct cttcacgcac gatgttttcg acttttttca gaacgcgcac
     2521 gccggtcggc agccaggtat ataacccgga ggccagcttg cggatcatcc cggcgcgcag
     2581 catcagctga tggctgatca cctcggcgtc ggcaggtgtc tccttgagag tggagagcag
     2641 gtattggcta gtacgcatgt tgttacggtt ccagttggaa ggtagaacag gctcaaggcg
     2701 agcctgggac aaaaaaaagt gatttagttt accagtgcaa aagaaatgtc aaaagagaag
     2761 ggcgtgaatt taacgcggtt ccagcgcaaa gacttcaaaa cctgcgtcgg tgacgcgcca
     2821 gcgaacgtta aaatcatgta gccagacggc ataggttttg cccgtttcct cacctttacg
     2881 ataggccggg cgtgggtcct gcgccagtac ttcgcggata aacagcgtta actgcggata
     2941 acgcttctcc agcgtcaaaa gctgcttttc gacctctgcg gtaaaactca ccgccatctc
     3001 tgccgctggc gcactttgcg catagctggc actggcatcg ggaagcgatt cggcaaaggg
     3061 gagatacggt ttgatatcca ctaccggcgt accatcgacc agatccagac tgccgagctt
     3121 cagaatcacg ctgtctttat ggcaaacaac ctctttcagc tctaccagcg acataccaat
     3181 tgggttaggg cggaaagtag agcgtgttgc gaaaaccccc attctggcgt taccgccgag
     3241 gcgcggtgga cgcacagtcg gacgccagcc gccttccatc gtttgatgaa agacgaaaag
     3301 gatccataaa tggctgaacg cttccaggcc gcgaacggcg tcggcctggt tgtagggagc
     3361 aatgagatgc agttcgccgt tggcgctttt taccagacct ggctggcgcg gaacggcgaa
     3421 tttttcttta tagggcgagc gaataacgcc tatttgctcg aactggaaac tgctcatttc
     3481 gccgtaatgt taagcgcaga gccgatacat acagcctgac gatagcagcc tggcgtaccg
     3541 ctggtgactt cgcagctatg cagtaatacc gcattggcct tcattttcga ggcgttgatt
     3601 tgcatccgct tacgcgcggt tggaatgctc ggcggagagt cctgattaga ggcctggcaa
     3661 gagtcgccac tgacttcacc gagatcgcgg aacggtttgc cgactaattc ttctgcattg
     3721 gtataaattc ggaccggcgt ggcgcgcggc gctttcggtt ttgcaggctc cgctttcggc
     3781 tggggtgcag tgctttgaac gggttcgaca ggggatctgc ttaacatgga acagccgctt
     3841 agcatgagtg ctactaaaca gatcggtaaa gcacgcatag tatttcctca atgtatgatc
     3901 aaaacgtcaa tattgaatca ggagcttgta aaaatgacaa gacgggcaag cgcccgtcct
     3961 gaatgatatt acaaattgtg gaaacagcct aaaaattacc agcctttaac agcgccgccg
     4021 ttaaacactt tgtttgctgc ttcgtaaact tcatcagact gataagcctg gacgaatttc
     4081 ttcacgttct cggcgtcttt gttatcttca cgcgttacga tcaggtttac gtacggggag
     4141 tctttatctt cgacaaagat accgtctttc gctggagtca ggccaatctg gctggcatag
     4201 gtggtattga taactgccag agcgatttgc gcgtcgtcca gagatcgagg cagctgcggt
     4261 gcttccagct caacaatttt cagatttttt gggttctcaa caacatcaag aacggtcggc
     4321 agcaggccaa cgccatcttt cagtttgatc aagcccactt tttgcagcag cagcagtgaa
     4381 cgaccaagat tagttggatc gtttggcacg gcaacctgcg aaccatcctg cagttcatcc
     4441 agtgatttga ttttcttgga gtaaccagca atcggataaa caaaagtgtt gcctactgcg
     4501 accagtttgt agccacgatc tttcagttgc tgatcaaggt acggtttatg ctggaaggcg
     4561 ttggcgtcga tatcgccttt gctcaatgct tcgtttggca gaacatagtc gttgaaggtt
     4621 accagctcaa cgtccaggcc atatttgtct ttcgcaactt tctgcgcgac ttctgctacc
     4681 tgctgttcgg caccaacaat cacgccgact ttaatgtggt ttggatcttt ttcatcctga
     4741 ccgcagccta ccagtgccag tgatccaatc agggctccca ctgccgcaaa ggttttgaat
     4801 ttgaacgcca taccttattc cttcttcaat tatttatgtt gtgttgaacg ttacttgcga
     4861 gtgacagccc ggacgatgcg gtcgcctgcg aactgaatta aataaaccag aatgaccagc
     4921 aataccagta ccgtattcat caccgtcgcg ttatagccga tgtagccata ctgatagcca
     4981 atctgaccta aaccaccggc accgactgca ccacccatcg cggaataacc gaccagggta
     5041 atcagggtga tagttgccgc attcaccaga cccggcaatg cttccggtaa cagcacttta
     5101 cggacgatct gcatcggcgt ggcacccatt gcacgggaag cttcaattaa cccggttggg
     5161 atctccagca gagcgttctc gaccatacgg gcaataaacg gtgctgcacc aacggttaac
     5221 ggaacaatcg ctgcctgcaa accaatcgat gtaccgacaa taacgcgggt aaacggaatc
     5281 atccatacca gcaagataat gaacgggatg gaacggaaaa tgttcacaat cgcagaaacg
     5341 gtacgataca gcttcgcgtt agcaataatt tgccccgggc gcgtaacata aagcaggacg
     5401 ccgaccggca gaccaatcac aaagccaaaa aatccggata cgaaggtcat tgccagcgtt
     5461 tcccatacgc cacgaaccag caaccacatc atcggctcag acataaccca gtacctctac
     5521 ttttacatgg tgttcctgca gccaggcaat ggcggcttgc gtatcttgtt gtgtgccgtg
     5581 catttcagtc agcatgatgc cgaacttaac gccaccggcg taatccatct gcgcgctaat
     5641 aatgttgttg ttgacgttga aacgacgcgc ggtttcagaa agcagcgggg catcgaccga
     5701 ttgaccggta aactccaggc gcaacatcgg cacgcagtca gtaaatggct ccgcttgcag
     5761 acgttcctgg taatcttccg ggatatccag atgcagggtc gactgaataa acttctgcgc
     5821 cagcggcgtt ttcggatgcg agaacacttc acttaccgtg tcctgctcga tcagttcgcc
     5881 attgctgatg accgccacgc aatcacaaat acgcttcaca acgtccattt cgtgggtgat
     5941 caacagaatc gtcaaaccca gacggcggtt aatgtctttc agcagttcga gaatagaacg
     6001 tgtcgttgcc gggtccagcg cgctggtggc ttcatcacac agcaatactt tgggattgct
     6061 ggctaatgca cgggcaatcg ccacacgttg tttctgccca ccggaaagat tcgacgggta
     6121 gctatcatgc ttatcgccaa gaccaaccaa tgacagcaat tccgtcacgc gacgtttgat
     6181 ctcgtctttc ggtgtgttgt ccagctccag cggcagagcc acgttgccaa aaacagtacg
     6241 cgaagagagc aggttaaaat gctggaaaat cataccaatc tggcggcgag ctttggtcaa
     6301 ctcggattct gacagcgtgg tcagttcctg gccatcgacc agcacgctac cctcggttgg
     6361 gcgctccagc aggtttacac aacgtataag cgtactctta cccgcgcctg aggcaccgat
     6421 aacgccataa atttgtccag ctggcacatg caggctgacg ttgttcaacg cctggatggt
     6481 gcgggtgccc tggtggaaca ctttggtgat attcgaaagt tttatcattg attatttatt
     6541 atcgtcatta agttagtcgt ggcatctcga atgcctgaaa cgggcaacgc cgtcaatgaa
     6601 atggatgtta aggcatccag acgtctaaat caatcaggtt tatgcgaaga gcactttctt
     6661 gcaggtcgaa acatgcgata ctagcgtccc atgccttatt aaggagctat aaaaggtggc
     6721 gaagagcgta cccgcaattt ttcttgaccg tgatggcacc attaatgtcg atcacggcta
     6781 tgtccatgag atcgacaact ttgaatttat cgacggtgtt attgacgcca tgcgcgagct
     6841 aaaaaaaatg ggctttgcgc tggtggtagt aaccaaccag tctggcattg ctcgcggtaa
     6901 atttaccgaa gcacagtttg aaacgctgac cgagtggatg gactggtcgc tggcggaccg
     6961 agatgtcgat ctggatggta tctattattg cccgcatcat ccgcagggta gtgttgaaga
     7021 gtttcgccag gtctgcgatt gccgcaaacc acatccgggg atgcttttgt cagcacgcga
     7081 ttatttgcat attgatatgg ccgcttctta tatggtgggc gataaattag aagatatgca
     7141 ggcagcggtt gcggcgaacg tgggaacaaa agtgctggtg cgtacgggta aacctattac
     7201 acctgaagca gaaaacgcgg cagattgggt gttaaatagc ctggcagacc tgccgcaagc
     7261 gataaaaaag cagcaaaaac cggcacaatg attaaaagat gagcggttga aataaaaatg
     7321 catttttccg cttgtcttcc tgagccgact ccctataatg cgcctccatc gacacggcgg
     7381 atgtgaatca cttcacacaa acagccggtt cggttgaaga g
//
LOCUS       DAZFWL020000191         7222 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFWL020000191 DAZFWL020000000
VERSION     DAZFWL020000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.191"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..113)
                     /locus_tag="ACHKNP_RS22705"
                     /old_locus_tag="pgaptmp_004541"
     CDS             complement(<1..113)
                     /locus_tag="ACHKNP_RS22705"
                     /old_locus_tag="pgaptmp_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706571.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNG"
     gene            complement(238..2238)
                     /gene="yehQ"
                     /locus_tag="ACHKNP_RS22710"
                     /old_locus_tag="pgaptmp_004542"
     CDS             complement(238..2238)
                     /gene="yehQ"
                     /locus_tag="ACHKNP_RS22710"
                     /old_locus_tag="pgaptmp_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HGU4706572.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEGLATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2235..3371)
                     /gene="yehP"
                     /locus_tag="ACHKNP_RS22715"
                     /old_locus_tag="pgaptmp_004543"
     CDS             complement(2235..3371)
                     /gene="yehP"
                     /locus_tag="ACHKNP_RS22715"
                     /old_locus_tag="pgaptmp_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU4706573.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3364..5643)
                     /gene="yehM"
                     /locus_tag="ACHKNP_RS22720"
                     /old_locus_tag="pgaptmp_004544"
     CDS             complement(3364..5643)
                     /gene="yehM"
                     /locus_tag="ACHKNP_RS22720"
                     /old_locus_tag="pgaptmp_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HGU4706574.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIKGPADFNDRV
                     DELFLSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEANMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(5654..6742)
                     /gene="yehL"
                     /locus_tag="ACHKNP_RS22725"
                     /old_locus_tag="pgaptmp_004545"
     CDS             complement(5654..6742)
                     /gene="yehL"
                     /locus_tag="ACHKNP_RS22725"
                     /old_locus_tag="pgaptmp_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HGU4706575.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1691 a         1953 c         1933 g         1645 t
ORIGIN      
        1 ccgttcagat tggcgctgaa tttcttcgat tcttctttgt caccgcagcc agcaagagag
       61 aaaaccagaa cagatgcaac aacgagggaa aacagcttat tgaaagcctt catgtaaaac
      121 tccattttat ttaatcaaga aactggtgac tctcaccagg ggctatatag aatatgccta
      181 ataccgtgac gtgagcagtc cggaactgga gtagaactct tagtaaaaag cactatttca
      241 tccttgttgc tgaagcatgg ggaataattg ttcgcaaagt aaaacaccgt tattcattgc
      301 ttctacccgt gcctcgcttt ctgtattacg aaattgtccc aacacatgtg ccagccgata
      361 aaaaccgacc gcggagaggt cattcgccag caactctgcc tgactaatag cgctctgttc
      421 ctgatagcgc cagccgttat ggagcagttg aataagtaac gcctggcagc gcatcagcaa
      481 ctgatgagcg gtagacggca caggcaaaac gctggcagaa ggtagcggtg ccacaggcgc
      541 agtttccgcg tccagcgccc aggcgcgggt ttttgtcatc atcacccgtg gttccagtgt
      601 caattgccca tcaacaaaac tgacaaagcc agaaaccaga ctcacggggt cgtctgtttg
      661 ttgcaaaagc gccgccatgc gttcaacggc aaaaggagaa caggcagatg ccggaaggga
      721 taacgtcagg agattatcct caccttcgcc gctgattacc tgcgcatcca gcgtctggcg
      781 gctgctatcc caaccgagcg aaatacactc agcgaccggc agaataaata agttatcgac
      841 ctgattaaga ggccgtatgc aggcgggggg acgctggcgt aaatattccc gcaaagccac
      901 aatgcccggc tggcgtaacg gcgcgctcaa catttgccag gcatcaggcg acagcggcac
      961 aacgctgctt aagcggttgc gggtagctaa cagcagctcg ccatcggcac tgcgttttgc
     1021 tgcttgtgaa acaatttgcc cacccgccag tgcgccagcc tgaaaactaa acagccgacg
     1081 cgtagctgcc ggtgagtttt cctgttcact tcgcggccaa ctgcgcgaaa ggtgcaaaat
     1141 actgccggtg tcgggatcgg taaaccagat gcgtaaacca taatgctcaa tatcctgcca
     1201 gcaacgcata cctaaagaca ccagccgcag atgatcaagc tttgcttctc cggcaatgcc
     1261 agagccaacg accgtgcgcc acggcacagg aggaacttca ccaacactgt cgcgccgggc
     1321 catctcttgt gcgcaattta atcgactgtt taatgccgca agctgacgta agcattctcc
     1381 ggcatgatag tggctggcgc gagtgtggaa ggcatcaacg cttgcccgta gctgtcgtag
     1441 tgattcactc acccagcgcc agttgcaggc ctctgccgcc tgcaatgcgc ggttgaatgc
     1501 tgcctcgtag tgaataagcg gctggctgat gccgccaagc cataatgcct ggcttaattg
     1561 ctgaacatat tgacgacacg tttggccttc ttcgctggca aacggatcgt cagatgatgt
     1621 gacgtgttcg ctgcgcatct gccagattaa atggttaaat tctgcttgct gcgctttggc
     1681 ctcgacgaag gcctgcacag ccagtacgac atgttcgcaa agtgtgcctt caatacaatc
     1741 acaacgggcg aaacgaatac tgctacggga ataaaaacgc acatcactca tcggtaagcg
     1801 ggcagaggga atttcgcccg gcgcacagaa caactcaatg gtgatgcctt tagcgaccag
     1861 cgcctgcgcg cgtttgcggg tggcatcggg cagggtagcc agtccttcca gccagattgc
     1921 cggatcccac tcttcttctt tttccgtggg ctggacggtg gcacaaagtc gttgataact
     1981 taacaccagc atcacgcgat gacggcacat gccgctggcc ccgcagctgc actgagcctc
     2041 tttcagtgcc tggctgttcg ccagctgggt acggacaccg tcactgaagg tggcgattaa
     2101 agcgccgttc tcatggctga tttccgggac gttgccattt tccagttcct taagactgcg
     2161 cttaacaaaa ccggcattgc ttaacgccgt cagtgcctgc ggtgtcagtt ctaataattc
     2221 cggacgtagt gaattcatga ctgaagattc tccgcaagcc atgatgccag ctcgcccggc
     2281 gtcatggcgg ctatttgtgc gccgacatta accagcgcct gggccgtatc gcggtcatag
     2341 caaggcgttg cggtgctatc gagcgctgcc agtcccagca ctttgatgcc gctctggaca
     2401 cactttttca cctgatgcgt cagcaatgat gatgaacccc cttcgtaaaa atcgctcacg
     2461 aggataatga cgctttttgc tggttgttca ataagttgcc gaccatactc cacggcactg
     2521 gcgatattgg tcccgccgcc caactgtact ttcattaata actctaccgg atcggcaacg
     2581 tctgccgtga gatcaaccac gcttgtgtca aacgccacca gatgggtacg aatgccgggt
     2641 aactgccaca aacaggccgc catcaccgca gagtggatca ccgagtccac catcgatccg
     2701 ctttgatcaa ccagtaagac cagttgccat tgttcgcttt ggcgtttaat gcggctgtta
     2761 aagcgggggg attcgatata caacttgccg tgttgcgggt gccagtgttg caggttggcg
     2821 cgcagagtac ttttgaaatc aaagtttcgc gccagtggaa ttaatgagcg gcgacggcga
     2881 tcgcggacac cagaaaaagc ctgacgaact tcctttgcca gtcgcgccat aatttcttca
     2941 acaacctggc gcactatctg gcgggcggca gccagtactt cgggattcat cagatgtttg
     3001 gtgtgcaaaa cggcgcgtag caggctttcc gaaggctgca tacgttccag cacgtcgaga
     3061 ttcgtcacca catcttcaat gccgtagcgc agtacggcat cgctttccag tcgctcaatc
     3121 acctgttgcg gaaacagagt gtgaatactg ttgatccact caggggtggt gagatttgag
     3181 ccacctaatc caccagagcg ttcaccacgc tggagccgtt caggatcgcg cccatacagc
     3241 cactccagcg cgtggtctat ctgccgggcg ttgtcatcca gcccacaaag cgtcgtttct
     3301 gccgcttcgc caagaattaa tcgccagcgt tgtagctcac gggtggtcag aagatcgttc
     3361 agttcagaca tggaaaactc cccagttttg cagcgatgcc agtgcctgct gttcgagttg
     3421 ttgatgatgt gcaatggcct gtggtggaca atgcaacggc atttgcagcg ccgaaacggg
     3481 aagttgcgcc agttgataat gctcaagcac ctgatgcgcc agcgtcccgc gttctcgtgg
     3541 tggtagccag gccatcgccg cgcgtaaatc gggcagggca ttgataaaat cggcatcact
     3601 tagttgattt aaatgactgc tgaaaccggc gataaatgtc ggctgacagg ccagttgatg
     3661 gcgggccagc gccagcaaac cgtgcagcgc ttcgccggat tgtgccgggg ataactgcgc
     3721 cagcatcgtc agcgccgctt cggcactggc gttaggatgt tccagacgca tcagtgcgcc
     3781 atgagccgcg ccgcgatcca gcgccggggc atgaattgcc tgactgcgtc gctccagtaa
     3841 ggctaccgcc gcagaaagag aaatgccggg gagttgcaca ccgctatgta gatcgcgcag
     3901 gatatgacac aacgcttgcc agctatgcag gtgagcgtga aactcctttt catctggtct
     3961 gccgttagat tcacatagcc acagcgtgcg atcgatagcc gcgcataacg tcgtttgtaa
     4021 tatctgcgcg ccttgcatac cgctaatttc atccagccgc cataaggcat atagcacttc
     4081 cagcgcctgg cccatttcgg caaattgatt ttcctgggcg attaataatg tgagttgctc
     4141 cagtaattgt tgactgaagg acgccagacc cgctaacgcc gcctggctaa gacatgtggt
     4201 gatactgccg attccacccg cgtccagcat attggcttct aatttatggc gtgcggcttc
     4261 ctggagtgtt gcaccaaagc aggcggcctc aatcaatgcc gcatgctggc taagcgggcg
     4321 ggttaatttc cagcgttctt caccgttgcc tgcaagtgtc agtgtgcttc cctgctggcg
     4381 tacaatccct gggatctcca gtattgccag ccgatgtaac acctgacttt gcgctagtcc
     4441 attggggtta aagcgattta aggtaagctc cgccggaaga gagattgcgg tacgttccag
     4501 ttcgcaggtg acatcttttg gcagaggcgg ttgtggcgta gaaggggcaa gtttaccgaa
     4561 tccgtcaccc gctaatgtgt ctatcaacgt tagcagaatc ggatcggtat cgggatgaat
     4621 aacgccgcga tagctccacg gcaacggcgc attcagggct tctttaatca gcgagcctgc
     4681 tatggcatcc agccagtcag tgcgtaacgg tagtgtatga ccgcgcaact gtgccagcgc
     4741 catcgcatgc agatgagcgg cagccatatc cgcggtcgaa gcaggcaact tgtgctggcg
     4801 caaacgggta aggatagttt ttagcagttg ttcaccggcc tgctgtaagc cccactgcca
     4861 gcaccagttt tgccagaccg gggcaggcat accggaaagg tatcctgcca gcacatcaag
     4921 gcgcttttca ctgtagggcg tgagataaca acctgtaatg gcatctgcca gcgagggcaa
     4981 ttccggcgtg ttaatgtcct gcgggcattc gcgccacatt tttgccagtg ccggagcgtg
     5041 ccagccaccg cagacgacta acacgtcgcc attattttgc tgcaccgccc atgcaatcca
     5101 gcgagccata aaggcttcgc gctgacgatt gagtgcatcg ccggggaaat cgccccgcag
     5161 ttgggcaaaa tagtgcgcca gcgcagaggg taatgcagtt tgctggcttt catcttcgaa
     5221 caagtgatcc cacagggtat cgctgttatc catgcgggtg gcacgcagca gtaaggcctg
     5281 gctttcatct tgcgtatcag gcgaatcgtc ctcttcttca ctctgcgccc agcaaggcaa
     5341 atcgatgaag taagtttgtg cctgaatacg acgtgcggct tgtagcgcct gccactccgg
     5401 cgaaaattca gcaaatggcg tccaggcacc acgcccgggg gctgcaccgt cctgatactg
     5461 gcaataactg taaatagcta ccggaagctg gtgggataaa aacagttcgt ctacccggtc
     5521 attaaaatca gccgggcctt taatcaacac gtatcgtggc cgctggcttt cgattaacga
     5581 tttcaccagc cgggcgcagg ccggactatg atgccggatg ccgacaatta acggctcgct
     5641 catgcatctt tcctcacggc aggcggtggc gggcttgata ataagcctgc cagtgagctt
     5701 ctttatgtgt ggcgactcgt tgttcgaagt aacggcgcag acgagcgcga tcttcttcgt
     5761 tatctttgac gatggttccg gcaatgcagt ccaccaggtc ggctggctca cctgcgcgat
     5821 tcgctaaaaa ccaggcgcgg acgcctacgg cgtgggccac gttcacggct tcagcggtgg
     5881 acataatcgc tgttagtgta tccattgacg ttttcttttc gccgttggca cgcagatcgc
     5941 ggaaggtgcg gaccagtaac tccagtaccg cgtcaggcac tttatgcgga ataccgctat
     6001 gggccagcaa acgcgccgaa gcactggcga caagctccag ctcctgagcg aaatccataa
     6061 tcggaaagac agtttcaaaa tcgaagcgac gctttagcgc ggcactcatc tcattgacgc
     6121 cgcgatcgcg ggtattggca gtggcaataa tattaaagcc ttcccgcgca tagagttgac
     6181 tggcttcacc agtgagttcc ggcaccgtca taactctgtc agaaagcata cctaacagac
     6241 aatcttgcac ttccagcggt gtacgcgtta tctcttcgaa acggacgatt ttgccgtcgc
     6301 gcattccctg gtaaagcggg gcagggacca gcgcttccgt tgatgggcca tgattgataa
     6361 gcagagcgta gttccagccg tacttgattt gatcttcagt ggtagatgcg ccgccctgga
     6421 tggttaaccc ggcatcgccg ctaatagcgg ttgccagtaa ttcagacagg agagattttg
     6481 cggtgccagg ttcccccacc aacatcaaac cgcgtccggt ggcaagagtg actaacatac
     6541 gttcgacagc ggagggacta atcaccactt tacggctaat attttgcgcg ctatcgccaa
     6601 ggataaaagc acgggccgca ggcaaactta actgccaacc gggcgggcaa ggtgcgttat
     6661 cattttgttt taattttgcc agttcatcgg cgtataacac agcagcaggc ggacgctgca
     6721 gatgattatt ctgcggtgac atatattcat ccctgaaatg tattaatgaa ataaacgata
     6781 ttttacgcgc aataaatgtt tggcgcatta gttgtttgag cggaaaatat ctggaaagca
     6841 gatcacagta aataaaaaaa cctgctggtg aggcaggttc ttttatttac tattttcagg
     6901 cctaacacca cagcctgtgc aacatcgtgt tccaacgaac gaattataac cacggctgga
     6961 ccccgttgca attaagataa tgagatttat aatcctgact gttgggacgt tgcgccggat
     7021 cgaacctatt tcaagtcctg gctaccggac ggggggtgct gcaggtggtg ccctgatccg
     7081 gagtgcaaca tcgtggtcca acatacgtaa accagtatcg acaggcagtg taagggtaag
     7141 gtgctggtgt tgcgctcctt aataaggaga gcggatggac tcgctgacac aaaagttaac
     7201 cgtgctcatt gctgtactgg ag
//
LOCUS       DAZFWL020000192         7213 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFWL020000192 DAZFWL020000000
VERSION     DAZFWL020000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.192"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(216..2999)
                     /locus_tag="ACHKNP_RS22730"
                     /old_locus_tag="pgaptmp_004546"
     CDS             complement(216..2999)
                     /locus_tag="ACHKNP_RS22730"
                     /old_locus_tag="pgaptmp_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU4706576.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVHDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3056..5428)
                     /locus_tag="ACHKNP_RS22735"
                     /old_locus_tag="pgaptmp_004547"
     CDS             complement(3056..5428)
                     /locus_tag="ACHKNP_RS22735"
                     /old_locus_tag="pgaptmp_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGU4706577.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSVVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5466..7151)
                     /locus_tag="ACHKNP_RS22740"
                     /old_locus_tag="pgaptmp_004548"
     CDS             complement(5466..7151)
                     /locus_tag="ACHKNP_RS22740"
                     /old_locus_tag="pgaptmp_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HGU4706578.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQSGVWNLADDICDISAVL"
BASE COUNT         1881 a         1639 c         1603 g         2090 t
ORIGIN      
        1 aacaatgcaa tatgaaattc ttgggtggtg gtaaggtgtt ttatgctgtt atttttatgc
       61 gcattctgtg tctcctgaat tatcacgtaa aaatcagacc ttaaaatatc actattagta
      121 cttgattatt attttgaacg catttataaa attattacat aaaaatagcg aatattgcta
      181 aaatccccgc caacgatgtg ttgacggggc tgttattatt ttggcaataa tactccggta
      241 taagtattta ccggatgaga aagatattgt ttaacggcag tgttaacatt ctctaccgtc
      301 atttgtttca acaattgctc ctgctcagtc catgctgcag gatcgtcata ttgaataaga
      361 ctatttacaa tagtgttcgc taattgttga acgctacgct gttggatatc gaggctgcgc
      421 tgaacgtttt gctggtattc attcagttct tgctcactga tccctttagc cagacgctta
      481 accatcactt cattcgctaa cgttaacagt tcatcatgtc gttctggttg acaagtaaaa
      541 gccagcaaat gactgatatc tttggcctga ggatcaaccg agaggcgaga agaaacgctg
      601 tatgctccag atgcctgttc acgaatatta acacgtagat cttttgccag tgcgacgtta
      661 aaagcatcga gcgccatacg cgtcgccaga ttaacaggtg tccgggaatc ataacgcttc
      721 cactgtgaaa cctgtgccac aggttcattt tgttctttta cagtaaccga tgcgttgtcc
      781 gtcgcgcgag ttaatggttt acctgcggct aatggcgaat cagagtgttt gattgatcct
      841 aagtaacgcg taattaacgc cacgagtttg tcttctgaga cattaccgac aatgacaaac
      901 gtgatatccg ctggagatga aaacaactgg cgatcggcag ccagcgcatc tgcggcagta
      961 aactgtacaa tctgattttc ttgcagtaat ttcgtgcggt catcagcata gcgcgtctca
     1021 tacatctgct gggcgaattt ctccgctgga cgctggtcga gcgttttcaa cgcctgaatt
     1081 tgagcatttt gtagcgatgc ccaaatattg tcgttaatcg tgctgtgggt gattcgctgg
     1141 ttaatcaact gaaaaccagg ttcagggtta ttagtccgcg cgctaacgga gagcaacgta
     1201 ttcatgccac tgactttact gctcatggtt accgaatttt ccgcactcca gcgtttcagg
     1261 ctggaagagg agagctcacc aacgccgctt ccgcttactg ctttatttgc cagcgcgata
     1321 agtgattttt gctgcgcagg gaaacttaaa tcgcctttat tcgatacggc agtaatttgt
     1381 agcttttgct cttcacccgc ggattttgcc agaataaccc tggcaccatt ggaaagtgtt
     1441 aatgatgtca gattctcagc cagcgtttct ttgctgctaa tttccgcctg tgggtcagcg
     1501 tctactgtta acgataaatt tctgcctggg aagatgtaag ccgccagctt tttgttggcg
     1561 tactcttttt ccagcgccag gatagccgca ggagacaatg cttttttggc ggcaagctca
     1621 ttgtttacca tttgctccca aaatgcgtcc tggttctttc ttaactgctg ccatttttcc
     1681 gccagacttt gcacggtaat ttgctgccac agacgtttcg aaagttgata tgtctcttcc
     1741 ggcgacaaga acggcgtatt atttaatgag ctggatgcca ggcgactggt cagcatacgt
     1801 aaatcacgtt cagcttgctg atcaaccgca tttttcagcc aggtgaggcg ggtagattta
     1861 acatcatcga gttcttcagc agaaaaacca tgctgatcaa tagttgccaa ctctgccatt
     1921 aatgcattcg cagcatcctg catattatcg tctcgtgcat ttacacggaa aaacagcgac
     1981 tgataatcgg gtgcaatttt aacgctgcgc gcagtgccgc cagaaatagt cttcaactcg
     2041 cccgactgta tgcgttcctg cagacgttga ttgaacagct gaactaacat gctccattca
     2101 gcttgttcga taaagctttg ctcatcgtgc acttgtacca ttggcaggcg ataatagagt
     2161 gcgatgccgt tcacccggtt ttctttatca ttaatgatat taaagcgcag gtggttttcg
     2221 gcttttgtcg gccagacgcg attttcagct gctttgttag ccggaagctt acttaaatta
     2281 tcctttatca gcgccagcgc ttctttactg tcgatatcgc caaccacgat aaaggtcata
     2341 ttatttggtt gataccagcg ttgataaaat tggcgcaatt gtgccggtgt gaccgtggcg
     2401 acagtatcca tcaggccgat aggttcacgg tctaaattac gggtattagc cagcaggaaa
     2461 gggcggcgcg cctgagaggt gcgccatttc gcatcctgat gggcacgcca ttcctcagta
     2521 attacgccac gttcagcgtc tacttcgagt ttttcaaagg ttgcggcatt actccattca
     2581 ctgaagattg ccatcacttg ttgcagattt tgtttctgcg tagtcggcaa actcacctga
     2641 tacaccgttt cgtcatagct ggtataggca ttaacatcgc gaccaaaacg caggcccatt
     2701 gactcaaatg tttcgatgac tttattaccc ggccatgttt ttgtgccgtt aaacatcata
     2761 tgttctacaa aatgagccac gccgcgctca ttgtcttctt cctgcaatga accggtatga
     2821 atttgcagcc ataaatttac ctgatctttt ggatgagcat gcggataaat catatatcgc
     2881 aagccattgc ccagttgccc ggtaattaac ttttcatcct gcggtaaggc ggcggcaatc
     2941 agccgcccgg ggagcaacag agttgccact aacgtcagta agaaacagag gtttctcata
     3001 attatctcca tgcgaaaacc gggcgaattt acccggttaa gtaaaatccg aactattaaa
     3061 atttcatgct gacatcaagc cagaaagtac ggccactggc gtaggtcgcc acaccggtat
     3121 tcgttgtatc aacagcggtt ttgctatcca atacattgag aatatcggca ctgatcgtca
     3181 ggttttgttg tttcagaaat tgtggcgtcc aggacaagcg ggtgtcccag gtcaggctgc
     3241 tatccagttt ttcgtcaaca tactgcttgt aatctgaata ttcgctgatg tattgcgcat
     3301 ttgtcttacc caggataatg cgagctttac gcgcttcttg ccaggccagc gtattcgccc
     3361 acactaaccc gctcggttga tgcgtgaaat ccatgtttaa ggagatcttt aatgggttat
     3421 taaaatctgc cactggaacg ctatcgtaag aaaccagatt accgttataa accacctggt
     3481 tatcaccggt attgctctcc tcataaccat tgtttaacga caagttgccc ttgctcttga
     3541 tatagctaaa gacaatttgc gggttaatat ctacctggct gatatgcagg ggttcggcca
     3601 gttcaaaact gaggttgaac gaatgcgttt ttgttttgcc gtcgttgtta tattcagtaa
     3661 tggtggtttt agtcgcgctg tcggtacgac tgcttttgct gatttgatca tgcgcttcac
     3721 ggtaaacata gtttgcgcgc gcaataacgt tcttaccgat tttctgctgc aatcccattg
     3781 ccagttcatc gttataaggc gttttcaaat cctgataacg cgtcagggtt ttattgcctg
     3841 ataccgattc cgtccagcta ttgcggatat cacgtaatcc catatcaaga atattcccgc
     3901 catagtaacg gttataacct gctgtaatca ttgaggtttg atcagcaaaa atatcccatt
     3961 ccgtcataaa gcgcggggag atattgtggt ttgacagata gttgtcatag tcataacgca
     4021 caccgggcat taacgacaca ttccgccagc taatgtgatc cgccatatac agcgtgtagt
     4081 tgtcaattcc caggctgcct ttacctttat ggtaaatggt atggttagtt ttctttcctg
     4141 cagcattaat cacataggat tcagactggt tatggcgttc agtccatgca tcggaataga
     4201 tgtattccgc gccgaagtag ggttgatgcg aaacatcacc cacggcgaat ttttgccagt
     4261 ccaggcgtgt tttgaaggtg taattatcta cagcctggga aatgtgtcct aatccgccac
     4321 gggtacaacg ccctgtaata tcaccatatg tacatgaaag ttcggtgtac cagatgtcat
     4381 gatcgtgacg ggtataatca ctaatatgat cccaaccaac ggtggtacgc agtttggccc
     4441 atgcgagctg cgtatccata tcccatgcca gaccgtagga tttattaccc atttcgcgat
     4501 cagactgcgg gaaggtgctg gtattataat cacggctgga gccggtatat tttaaggtta
     4561 aatcgtgggt aaagcggtcg ctggcaaacc aggtaaattt gctcaatgca gtatcgataa
     4621 cgtttttata ctgtgcccga ccggcgacaa tgccgtcatt cgaaacataa tccgcgcggg
     4681 tgatatcaga ctggcggcgc gataaaccgg cggtaacgcc gaagttatca gcgagttcct
     4741 gattaaacga caaggtataa aagttctttt taaaatctgg ggagtaataa gtacttcctg
     4801 aagagccttg attaaatgcg ctcttgttat tctcatcgat atgcgatgtt aaccagtccg
     4861 aacgcgtagt gcgataaccc aatttcacct tgctatcatc agcgttgaag cgtttgatct
     4921 ttgcatcaat taccccgcca ttgaagcgac caaattcaac cggcacaaca ctgtcataaa
     4981 gcgtcacatt gtccagtaag ctgacatcaa gataataccc ctgtgacatc ccgctaatat
     5041 tggttgcact actggcatcg gactcattcg ctgggttcaa gttattagtg gcactaatac
     5101 cgtcaatcaa ataggcattc tgatagggcg acgcaccgtg aatagagatt ttctccgggc
     5161 gaatatctcc ctggttcaac gaggtacttt gcgttgaatc catgcgtacc gcagggttgg
     5221 ttctcagcag atcgctgata ttgccattcc cggtcggtaa acgctcgatg ctttcactgg
     5281 tgtaatgcgt gttgccgttt acgggtacag ggacaggagc atagaccgtc atctcttcaa
     5341 aaacatgcat gtacatgttt ttgtcatcga cggcctgcgc cacatcagcg ccacataaaa
     5401 tgacgccagg aataagaact cgcttcattt atttatatct ccgtacagca gggcttttat
     5461 tgtttttata aaaccgcgct aatgtcacaa atatcatctg ccaggttcca gacaccggat
     5521 tgatgtgtaa ccataatgac gccgcttgtg ggtagttttt cacgcactaa acgcagtaag
     5581 cggatagcct cttgttcctc aagatgagag gtagtttcgt caagaaatat ccatttcgga
     5641 cgtcgtaaaa ttaatcgtgc cagggcgata cgttgttttt cgccgctgga aagaatatct
     5701 ccccagcgat cgtggtcatg aatacgcgca gccaatttcc ccagaccaac ctgatgcagt
     5761 acttcgctca acgatttatc gtctacgggc aggggaagtg ctttacaaat aatctctttc
     5821 agtaagccgg ttttgattaa cggtgtttgt gacacatacc aactgtcagc aggagaagaa
     5881 atatcacctt taaaccacgg ccagcagtga gataatgttt taagcagtgt ggtttttccc
     5941 gcaccagagt aacctttcag taatagccat ttgcctggcg aaacatgaaa gttcaggttc
     6001 tctaatatga tcttattatc aggagtacga atactcgcgt cagccacttg taccgcatgt
     6061 tggcaatttt taggtttatt cgtagggcgc tgttcggtga gttgatggaa ctcatacaaa
     6121 cgatcgataa ccgcagccag ttcagcaagt tcgtcatatt tataaataaa ccagcttaaa
     6181 ttgttcgata ccagcataaa tgcctggcgc gatttcatca gtccgcccag attgatctgc
     6241 ccgctaataa actgcggcaa taacagaaag tagggaagaa cgctaagcga gcgcgaataa
     6301 atattctgcc agtaatcaag ccaccgctga cgattcatta aacgatgcca gttctcttta
     6361 atggtatgaa aattgtcgct caactcctgg cgttgtagag actcagcatt gcttagcgca
     6421 atcagttctg cctgcttatt atgctgcaca agatttgttc gaaatgtcgc ttcgctacgt
     6481 tgtttttcca cattaagcgg acgaatacgt ttaccaacct tatgggtaaa taaagttcca
     6541 ccgatcacaa tgagcacaac ggtatagacc atatacccct ggatattcca ttctgttcca
     6601 ccaacagtga atgagagcgt acccgcgctt tgccaaagaa taacggtaaa ggtgatcagc
     6661 atgctaagtg actggatgaa gccaaatgaa aggctgagcg ttttgctgat cagtaagaga
     6721 atgtcttcag cgatacgttg gtcagggtta tctgtatttt tatgctcgcc gtagatctgc
     6781 gtgaagtagt agtttttatc tgcaaaccac cgattaaggt agtaatctgt taaccattcg
     6841 cgccagcgaa tagtcagtag tttaattaac catgttttgt ttacggatat taatacaaag
     6901 atccccagca gggcagggaa ccaaaggaca agctgccaaa gcttatcggt ttctttctgg
     6961 cttagcgcat tgaaaaaatc attattccag tcgtttaacc agacctgaat tttaaccacg
     7021 ccaaggatca tggcgaggat aataatgatt aacaacactg aagttttatt attcttacgc
     7081 agccaaaagg gcttcagcag acagagatac tttgctatca acatacaaaa cgtaatggga
     7141 atggttatca ttagcgaaaa ttgattgtgc catccaatga ttatctatgg caatgattta
     7201 ctgatgaaat tta
//
LOCUS       DAZFWL020000193         7196 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFWL020000193 DAZFWL020000000
VERSION     DAZFWL020000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.193"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1176
                     /gene="tssI"
                     /locus_tag="ACHKNP_RS22745"
                     /old_locus_tag="pgaptmp_004549"
     CDS             <1..1176
                     /gene="tssI"
                     /locus_tag="ACHKNP_RS22745"
                     /old_locus_tag="pgaptmp_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HGU4706579.1"
                     /translation="QEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQMDGWRNNAET
                     ARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGRQGAGTALENH
                     FAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVRFNWDRYNPADQ
                     DSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSL
                     PGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLNDRTTTVKHDHR
                     ETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGSVHVDAVNNGG
                     EGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
     gene            1204..1665
                     /locus_tag="ACHKNP_RS22750"
                     /old_locus_tag="pgaptmp_004550"
     CDS             1204..1665
                     /locus_tag="ACHKNP_RS22750"
                     /old_locus_tag="pgaptmp_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HGU4706580.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            1685..6484
                     /locus_tag="ACHKNP_RS22755"
                     /old_locus_tag="pgaptmp_004551"
     CDS             1685..6484
                     /locus_tag="ACHKNP_RS22755"
                     /old_locus_tag="pgaptmp_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
                     /protein_id="HGU4706581.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEIPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTESNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRSEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVGGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLNFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPDDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDAQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKGTHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAALGQLSRYTAADGAEWL
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERAETGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYILDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEETTWGGLLPSLVREYRYDSA
                     GNVSAVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSR
                     DQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNGFELISQQFSRVRQAHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTGYPDPRGEWDPEADPGLLYAGQWQDAESGLCYNRFRYYEPE
                     TGMYLVSDPLGLQGGEQTYRYVPNPLGYIDPLGLAKTSVPAEKISLSDKARDLFRQGK
                     VREALDVHYEDLVRRKLGGISQEIAGREYDVVTDKIIAQVKRTYSSIDNPKNFLSKST
                     RTQIKKTIELAEEQGKEAQFWFKYGVSPKVREYIESKGGKVILGMGN"
     gene            6486..6851
                     /locus_tag="ACHKNP_RS22760"
                     /old_locus_tag="pgaptmp_004552"
     CDS             6486..6851
                     /locus_tag="ACHKNP_RS22760"
                     /old_locus_tag="pgaptmp_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004014665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706582.1"
                     /translation="MSRYILIRGWFECDFEQVKKIKEANEEFFKHSEKYLLPENTKRL
                     YLNGWCYPEYPINWVSVISLGVNINQIAFDYIKDMIVNVSKTERLDGYFRVNDDEDNL
                     FLEWIVKDGYLEEIILSGS"
BASE COUNT         1826 a         1844 c         2173 g         1353 t
ORIGIN      
        1 caggaaggcc agcaccagga ctaccagcgc acgcagtatg aagtgtatga ctaccccgga
       61 cgtttcaaga gcgcccacgg gcagaacttt gcccgctggc agatggacgg ctggcgaaac
      121 aacgcagaaa ccgcgcgggg aatgagccgc tcgccggaga tatggccggg acggcgaatt
      181 gtgctgacgg ggcatccgca ggcgaaccta aaccgggaat ggcaggtggt ggcaagtgaa
      241 ctgcacggcg aacagccaca ggcggtgcca ggacggcagg gagcggggac ggcgctggag
      301 aaccattttg cggtgatccc ggcagacaga acatggcgac cacagccgtt gctgaaaccg
      361 ctggtcgacg gcccgcagag cgctgtcgtg acaggaccgg caggcgagga aatcttctgc
      421 gacgaacatg gtcgcgtgcg ggtgaggttc aactgggacc gttataaccc ggcagaccaa
      481 gacagttcgt gctggatccg tgtggcacag gcgtgggcag gcaccggttt tggccacctg
      541 gcgataccgc gtgtgggtca ggaggtgatt gtagacttcc tcaacggcga tccggaccag
      601 ccgatcatta tggggcgcac ctaccaccag gaaaaccgca cccccggcag cctgccggga
      661 acaaagacgc agatgaccat ccgctccaaa acgtacatgg gcagcggatt taatgagctg
      721 aagtttgatg atgcgacagg gagagaacag gtctacatcc acgcgcagaa gaacatggat
      781 accgaagtgc tcaacgaccg taccaccacc gtaaaacacg atcaccgcga aaccgtaaaa
      841 aatgaccaga cggtcacgat ccaggaaggt aaccgccttc ttacggtgga aaaaggccac
      901 aagatcaccg gagtactgaa agggtcttta tctgaggatg tctttcagga cagaggcacg
      961 attgccggtt cggtgcatgt tgacgctgta aacaatggtg gcgaaggcga cggtatacag
     1021 gcttatacgg cgattaagga aattttgctg gctgtggagg aaagcaaaat tgcgctgacg
     1081 ccggatggca ttcagctaca ggtcggggaa tcgacggtaa tcaggctgtc gaaggatggc
     1141 atcaccatcg tgggcggttc tgttttcatc aactgattca ctcagattat cagggagtat
     1201 tttatgcagt tcacttttaa tgaaggccac attcaactgc cgtctcaatg gcaggatcag
     1261 tcaatgcagg tcctggtgtc cacagataac agtggcatca acctggtcat tacccgcgaa
     1321 gccgttccac agggaaccct gacaccagag ctgtaccagg aaacgctggc gctgtaccag
     1381 ggcaaacttg atgggtacac cgaacatgcc tgccgggaaa tcactctggc agaggccccg
     1441 gcctggctgc tggattattc ctggaacggt ccggaagatg aggggaatca ggggcggatt
     1501 agccagatag ccgtattcca gcggcgtgga gatacgctgc tgaccttcac tttcagcacc
     1561 tccttatccc tgaagaacag tcaaaagacg atgctgctgg aggtgataaa gagcttcacc
     1621 ccgttgccac cggagaatga catccaaaag gaccagccgc gctgacataa acgagggata
     1681 acacatgagt gaaggaccag gcgggccaca gggagcgacc gcaggcggta cgctggcaat
     1741 gcgaatgctg tcacagcagg cgatggtcgc cagccagatg aaacgggcag ccaacgacaa
     1801 agccattgca cagatgctgg cagcaaagaa gtccggccca cctgccgcca ggctgggcga
     1861 tgaaattcag cataaaagtt ttctgggggc actggcaggg gccgtgctgg gggcgatagt
     1921 gaccatcgca gaaggttgcc tgattatggc cgcctgcgcc accggccctt atgcgctggt
     1981 tctggtgcct gcgctgatgt atgccagcta taaggcgagt gattatgtgg aggagaaaca
     2041 gaaccagctt gaatcatgga taaacagctt ttgtgacacg gacggcgcca tcaataccgg
     2101 ttctgaaaat gtaaacatta acggaaagcc cgctgcaagg gccgccgtca cccttccccc
     2161 tcctccccca cctggagcaa tacctgaaat cccacagggg gaaccctcat ggggtgatat
     2221 tgccactgac ctgcttgaat cggcagcgga aaaagcagta ccactggcga aggcctgggg
     2281 gaacgctgtt atcaccctga cggaaagcaa tgccggtttt atggatcgcg tcagcgccgg
     2341 cgcatcgctt ctgtttcccg ccggtccggt attaatggag tttgccacca tggtgggcgg
     2401 gcgtagcgaa atcaaaaaag atgtggattt cccggaagcc ggtgaggaca cggcgctctg
     2461 cgacaaggag aacaaaccac cgaggatagc ccagggcagt agcaacgtct ttatcaacaa
     2521 tcagcctgcc gcgcgcaagg gcgacaaact ggagtgcagt gcggcgatcg tgggaggttc
     2581 gccggacgtc tttattggcg gtgaacaggt gacctatctg gatatccagc cggagttccc
     2641 gccgtggcag agaatgatcc tggggggaat aacgatagcc agctatcttc tgccgcctgc
     2701 gggactgctg ggaaaactga agaatctggc gagactcggc aaactgggaa acctgctggg
     2761 gaaaagcggg aagctgctgg gcgcaaagct cggcgcgttg ctggggaaaa caggtaagtc
     2821 gttaaaaagt attgccaata aagtcatcag atgggtaaca gatcctgtcg atccggtaac
     2881 cggcgcatac tgcgacgaac gtaccgactt caccctgggc cagaccctcc ccctctcctt
     2941 cacccgtttc cacagttctg tactgccgct gcatggcctg accggcgtgg gctggagcga
     3001 ctcctggagc gaatacgcct gggtgcgtga acagggaaat cgggtagata ttatcagcca
     3061 gggagccacg ctgaacttcg ccttcgacgg tgacagtgat acggcggtta acccgtatca
     3121 cgcccagtac attctgcgcc gccgcgacga ttacctggag ctgttcgaca gggatgcact
     3181 gagcagccgc ttcttttatg acgcctttcc gggaatgcgt ctgcgccacc cggtgactga
     3241 cgataccagc gatgaccgcc tggcacacag ccccgacgac cggatgtata tgctgggcgg
     3301 gatgagcgac accgccagca accgcatcac gtttgagcgc gacagccagt accggatcac
     3361 gggtgtcagt cacaccgacg ggatccggct taaactgacg taccacgcca gcggctacct
     3421 gaaagccatt caccgcacgg ataacggcat acagacgctg gcgacctacg aacaggatgc
     3481 gcgggggcgg ctgacagaag cggatgcgcg gctggactac cacctgtttt atgagtacga
     3541 cgctgcggac cggatcatcc gctggtccga taacgaccag acgtggagcc gtttcaccta
     3601 cgatgcacag ggccggtgcg tgaatgtcac cggggcggag ggctattaca acgccacgct
     3661 ggactatggt gacggctgca ccaccgtgac ggacggcaag ggcactcacc gttattacta
     3721 tgatcctgac ggcaatattc tgcgggaaga agcgccggac ggcagtacca ccacgtatga
     3781 atgggatgaa ttccatcacc tgctggcccg ccactcccct gccgggcggg tggagaagtt
     3841 tgaatacaac gccgcactcg gtcagttaag ccgttacacg gcggcagacg gcgcggagtg
     3901 gctgtaccgc tatgatgagc gcggcctgct cagcaacatc accgaccctg ccgggcagac
     3961 atggacgcag cagtgtgatg agcgcggcct gccggtaagc ctggtgtcgc cacagggcga
     4021 agagacccgg ctggcataca ccgctcaggg gctgctgtcg gggatattcc gccaggatga
     4081 acggcgtctg ggcatagagt acgaccacca caaccggccg gaaacactca ccgacgtgat
     4141 gggccgtgaa caccacaccg aatacagcgg tcacgacctg ccggtgaaga tgcgcggccc
     4201 cggcggtcag tcagtgcggt tacagtggca gcagcaccat aaactgagcg gccttgagcg
     4261 ggcagaaacc ggcgcagaag gattccgcta tgaccgccac ggcaacctgc tggcgtacac
     4321 ggacggtaac ggcgttgtct ggacaatgga atacggcccg ttcgatttgc cggtggcgcg
     4381 aacggacggt gaaggccacc gctggcagta ccgctacgat aaagacacgc tgcagctcac
     4441 agaagtcatt aacccgcagg gcgagtcata ccgttatatt ctggacaact gtggccgggt
     4501 gacggaagag cgtgactggg gcggcgtggt ctggcgttac cgctatgacg ctgatggcct
     4561 gtgtaccgcc agggtcaacg gcctggagga aaccatcctc tacagccggg acgccgcagg
     4621 ccgcctggca gaagtcatca cgccggaagg caaaacgcag tatgcgtatg acaaatccgg
     4681 caggctgacg ggtatcttca gcccggacgg cacatcacag cgcaccggct atgacgaacg
     4741 cgggcgggtg aatgtcacca ctcagggccg acgggccatt gaataccact accccgatga
     4801 acacaccgtt atccgctgta tcctgccacc ggaagatgaa cgcgacagac accccgatga
     4861 atccctgctg aaaaccacgt accgctacaa cgccgccgga gaactgacag aggtcattct
     4921 gccgggggat gagacgctga cgttcagccg tgatgaggcg ggacgtgaag tgctccggca
     4981 cagtaaccgg ggttttgcct gtgaacaggg ctggaatgca gccggccagc ctgtcagtca
     5041 gcgcgccgga ttttttccgg aggaaaccac atggggcggg ctgctcccct cactggtacg
     5101 ggagtaccgt tacgacagcg cgggcaatgt gtcggctgtc accagccggg aagattacgg
     5161 acgggaaacc cggcgggagt accggctgga ccggaacggc caggtcacgg cggtgacagc
     5221 ctcaggcacc gggctgggct atggcgaagg cgatgagtcc tatggctatg acagttgtgg
     5281 ctacctgaag gcgcagtctg cgggcaggca ccggataagt gaagagactg accagtatgc
     5341 cggaggccac cggctgaaac aggccggaaa cacgcagtat gactatgacg ccgcaggccg
     5401 gatggtcagc cgcaccagac accgtgacgg ctaccgcccg gaaacagagc ggttccggtg
     5461 ggacagccgg gaccagctga ccgggtattg cagcgcacag ggtgagcagt gggaataccg
     5521 ccacgacgcc agcggcagac gaacagagaa acgctgcgac cggaagaaaa tccgcttcac
     5581 gtacctgtgg gacggcgaca gtattgcgga aatccgggaa taccgcgatg ataaactgta
     5641 cagcgtacgg cacctggtgt ttaacggctt tgagctgata agccagcagt tcagccgggt
     5701 acggcaggcg catccgtcgg tggccccgca gtgggtgacg cggacgaatc atgcggtgag
     5761 cgacctgacg ggccgcccgc tgatgctctt taacagtgaa ggtaaaaccg tctggcggcc
     5821 gggacagacc agcctgtggg ggctggcact cagcctgccc gcagacaccg gctacccgga
     5881 cccgcgcggg gagtgggacc cggaagccga ccccggcctg ctgtatgcgg gacagtggca
     5941 ggatgcggaa tcggggctgt gctataaccg gttccggtac tacgagccgg aaaccggaat
     6001 gtacctggtg agtgatccgc tggggttgca ggggggtgag cagacttacc ggtatgtgcc
     6061 taatccactg gggtatattg atccgctggg gctggcaaaa acttctgttc ccgcagaaaa
     6121 aataagcctt tcggataaag caagagatct ttttagacaa ggaaaagtaa gagaagcatt
     6181 agatgttcat tatgaggatc ttgtacgaag aaagcttggt ggtatatctc aagaaattgc
     6241 cgggcgtgaa tatgatgttg ttactgacaa gatcatagcg caggtcaaga ggacttatag
     6301 ctctatagat aatccaaaaa acttcttaag taagtctact cggacacaaa ttaagaagac
     6361 aattgagctg gctgaagaac aggggaaaga ggctcagttt tggtttaaat atggagtaag
     6421 ccctaaagta agagaatata tagaaagcaa aggtgggaaa gttatattgg gtatggggaa
     6481 ttaacatgtc aagatatata cttattagag gttggtttga gtgcgacttt gaacaagtaa
     6541 aaaaaataaa agaagctaat gaagaatttt tcaaacattc tgaaaaatat ttattaccag
     6601 aaaacacgaa acgactttat ttaaatgggt ggtgttaccc agaatatcct atcaactggg
     6661 ttagcgttat ttccttaggt gttaatataa atcaaattgc atttgattat ataaaagaca
     6721 tgattgtaaa tgtttcaaaa actgaaagat tggatggtta ttttcgtgtg aatgatgatg
     6781 aagataattt attcttagaa tggattgtaa aagatggtta tttagaggaa ataattttgt
     6841 ctggttcata aattcatctt ctttgttgag aagattaact gcctaggtga aattatcctc
     6901 tatgaccggg acgctgcagg ccacctggca gaagtcatca cgcaggaaga caagtcgctg
     6961 tatccctttg aaaaatccgg caggctaatg ggcatcttca gcccaggcgg cacatcacag
     7021 cgaatttgaa ggcaaatgcg aaaattagtg atcttgaagt tcatacagta ttggtaaaaa
     7081 caggcgaagc ttttccaatg tgtaagaatt ttcaaatttc aatatttggc gctgttgtta
     7141 caagcgggtg attatatgtg tgaattaatt aatgaagcat caaaatatgg gaaagt
//
LOCUS       DAZFWL020000194         7190 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFWL020000194 DAZFWL020000000
VERSION     DAZFWL020000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.194"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..4219
                     /locus_tag="ACHKNP_RS22765"
                     /old_locus_tag="pgaptmp_004553"
                     /pseudo
     CDS             1..4219
                     /locus_tag="ACHKNP_RS22765"
                     /old_locus_tag="pgaptmp_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            4442..4708
                     /gene="hipB"
                     /locus_tag="ACHKNP_RS22770"
                     /old_locus_tag="pgaptmp_004554"
     CDS             4442..4708
                     /gene="hipB"
                     /locus_tag="ACHKNP_RS22770"
                     /old_locus_tag="pgaptmp_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HGU4706583.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            4708..6030
                     /gene="hipA"
                     /locus_tag="ACHKNP_RS22775"
                     /old_locus_tag="pgaptmp_004555"
     CDS             4708..6030
                     /gene="hipA"
                     /locus_tag="ACHKNP_RS22775"
                     /old_locus_tag="pgaptmp_004555"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HGU4706584.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            6342..6550
                     /locus_tag="ACHKNP_RS22780"
                     /old_locus_tag="pgaptmp_004556"
                     /pseudo
     CDS             6342..6550
                     /locus_tag="ACHKNP_RS22780"
                     /old_locus_tag="pgaptmp_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            6982..>7190
                     /locus_tag="ACHKNP_RS22785"
                     /old_locus_tag="pgaptmp_004557"
     CDS             6982..>7190
                     /locus_tag="ACHKNP_RS22785"
                     /old_locus_tag="pgaptmp_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4706585.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSR"
BASE COUNT         1979 a         1544 c         1946 g         1721 t
ORIGIN      
        1 cgttcaggac ggttcccacg gtgtcgtcaa cgtgaccgat aaagggcatt ggaatttcct
       61 cggaacgggc gaagctttcc gctatatcta tatcggtgat gctggcgacg gtgaacttaa
      121 tgtctcgcgt gaaggtaaag tagattcggg aattatcact gcggggatga aagaaacagg
      181 cacaggcaac attactgtta aggataagaa ctccgttatc actaatctcg gaactaatct
      241 tggttatgac ggccacggcg aaatgaatat cagtaatgaa gggcttgttg tcagcaacgg
      301 aggaagctca ctcggttatg gagaaaccgg cgtcgggaat gtcagcatca ccacgggggg
      361 gatgtgggag gtcaataaga atgtctatac caccattggt gttgcgggcg tcggaaacct
      421 caatattagc gatggcggta agttcgtatc gcaaaatatt acttttttgg gcgataaagc
      481 aagcggtatc ggcacactga acctgatgga tgcgacatca tcgttcgata ctgtgggtat
      541 caatgtcggt aattttggta gcggtatcgt aaatgtcagt aatggtgcca cccttaattc
      601 aacgggctat ggatttatcg gaggaaatgc ctccggtaag gggatagtta atatttcaac
      661 ggacagtctc tggaatttaa agacatcatc tactaacgca caattgctac aggtcggtgt
      721 attaggcaag ggtgaactga atattaccac cggaggtata gttaaagcgc gtgatacaca
      781 gatagctctc aatgacaaaa gtaagggcga cgtgagggtg gatgggcaga actctcttct
      841 tgaaacattc aatatgtacg tagggacatc tggtacgggt acgttaaccc tgacgaatag
      901 cggcacgctg aatgtcgaag gtggagaagt ttacttaggt gtttttgaac ctgctgtagg
      961 aacgctaaac attggtgcag ctcacggtga ggcggcggca gatgccgggt atataaccaa
     1021 cgcgacgaaa gtagagtttg gttctggcga aggtgttttt gtctttaatc atactaataa
     1081 cagtgatgcc ggctaccagg tcgatatgct gattacaggt gacgataaag acggaaaagt
     1141 gatccatgat gcaggccata cggtgttcaa tgcagggaat acttatagcg gtaaaacgct
     1201 ggtcaatgac ggcctcctga ccattgcgtc tcatacggca gatggggtaa cgggcatggg
     1261 ttcgagtgaa gtcaccattg caagccccgg tacgctcgac attctcgcat caacgaacag
     1321 tgcaggagat tacacgctga ccaatgcgct caaaggcgat ggcttgatgc gagtgcagct
     1381 gtcatcctac gacaagatgt ttggctttac ccatgcaaca gggactgaat tcgccggtgt
     1441 tgcccaactg aaagacagta ccttcactct ggaacgcgac aacaccgctg cgcttactca
     1501 cgcgatgttg cagtctgaca gtgaaaatac cacatcggta aaagtgggag agcaatccat
     1561 tggtggactg gccatgaatg gcggtacgct cattttcgat acggatattc ctgctgcgac
     1621 gcttgcagag ggatatatca gcgtcgatac gctggttgtc ggcgcgggtg actacacctg
     1681 gaaaggccgt aactatcagg taaacgggac gggcgacgtg cctaaaccgt ggaatgatcc
     1741 catggcgaat aaccctctga cgacgctcaa tttgctggaa cacgatgata gccatgtcgg
     1801 cgttcaactg gtgaaggcgc aaacggttat tgggtcgggt ggctcattaa cgttacgtga
     1861 tttacagggc gacgaggtgg aagcggacaa aacgttacac attgcgcaaa acggaacggt
     1921 ggtcgccgag ggtgattatg gattccgcct cacgaccgca ccaggtgatg gtttgtacgt
     1981 taactatggg ctgaaagcgc tgaacatcca tggtgggcaa aagctgacgt tagccgaaca
     2041 tggcggagcc tatggcgcaa cggccgatat gtcggcaaaa atcggtggtg aaggggatct
     2101 ggcaatcaat acggtgcgac aggtttcgct ttccaacggt cagaacgact atcagggggc
     2161 aacctacgtt cagatgggga cattacgtac cgatgcggat ggtgcacttg gcaacacccg
     2221 ggaactgaac atcagcaacg cggccatcgt cgatcttaat ggatcgacgc agacggtaga
     2281 gacattcacc gggcagatgg gttcgactgt tttgttcaaa gagggggcgc tgacggtaaa
     2341 taaaggtggg atcagtcagg gtgaactgac aggtggcgga aacctgaatg ttacaggggg
     2401 aacgctggct atcgaggggc ttaatgcacg ctacaatgcg ttaaccagca ttagcccaaa
     2461 tgcggaagtc agcctcgata atacgcaggg gttaggcaga ggaaatattg ccaataacgg
     2521 tctgttaacg ctaaaaaacg tgactggcga actgcgcaat agcataagcg ggaagggtat
     2581 cgtgagcgca accgccagga cagatgtaga gctggatggc gataatagcc gctttgtggg
     2641 gcaattcaac attgatacag gcagcgcgct cagcgtcaac gagcagaaaa acctgggtga
     2701 tgcttccgtt atcaataatg gcctgctcac catctccact gagcgtagct gggcgatgac
     2761 gcacagtatc agcggtagcg gtgatgtgac aaaactgggt accgggatcc tgactcttaa
     2821 caacgattcc gcggcgtatc agggtacgac ggatatcgtg ggggggggga aattgctttc
     2881 ggttccgact ctgccattaa tatggcaagt caacacatta atatccataa cagcggtgtg
     2941 atgtcgggaa atgtcaccac tgcaggtgat gtgaacgtta tgcctggggg gacactgcgt
     3001 gtcgctaaaa ccacaatcgg cggcaacctg gagaatggcg gcacggttca aatgaatagc
     3061 gaagggggga aaccggggaa tgtactgacc gttaacggca actataccgg aaacaatggc
     3121 ctgatgacgt tcaacgcgac gctgggcggc gataattcgc ccaccgataa aatgaacgtg
     3181 aaaggcgata cccaagggaa cactcgcgtt cgggttgata acattggcgg cgtcggtgca
     3241 caaacggtca acggtattga actcattgag gttggcggta attctgcagg taacttcgcg
     3301 ctgaccaccg gaactgtcga agctggggct tacgtctaca cgctggctaa agggaagggg
     3361 aatgacgaga aaaactggta tctgaccagt aaatgggacg gcgtaacgcc agcggataca
     3421 cccgatccca tcaataatcc ccctgttgtg gatccggaag gcccatcagt ttatcgcccg
     3481 gaggccggaa gctatatcag caacattgcc gcagccaact cgctgtttag ccatcgttta
     3541 cacgaccgtc tgggtgagcc gcagtatata gattcactgc attctcaggg gtcggcaagc
     3601 agtatgtgga tgcgtcatgt cggagggcac gaacgttcaa gggccggtga cggtcagcta
     3661 attactcagg ctaaccgcta tgtattgcag ctaggcggcg atttggcgca gtggagtagc
     3721 aacgcgcagg atcgctggca tgacggcctc tgttgagggt gggtatacct ttgaagcggg
     3781 aacatttaga ggcagcgaag ggacgctgaa tacctggtac gtccagccac aggcgcaaat
     3841 cacctggatg ggtgtgaaag attctgacca taatcggaaa gacggaacac gcattgaaac
     3901 ggaaggcgac ggaaacgtgc aaacgcgact tggggtgaaa acctacctga atagccatca
     3961 caagcgtgac gatggtaaac agcgtgagtt ccagccttac attgaagcga actggatcaa
     4021 caatagcaaa gtctacgccg tgaagatgaa tggtcaaacc gtaagccgtg atggtgcgcg
     4081 taatctcggt gaagtacgta ccggggttga ggcgaaagta aataacaacc ttagcctgtg
     4141 ggggaatgtc ggtgtgcaac taggtgataa aggctatagc gatactcagg gcatgctggg
     4201 agtgaaatat agctggtaaa ccgtataagc cgcatgtcga gatggcatgc ggcttaatat
     4261 tgccgacttc aaacggcgca tcagcgcctt ttttaaatcc tcctttttat ccgcgatcgc
     4321 ggatatcgca gcgtttatcc cgtagagcgg ataagacgtg tttccaaatt gacttatcct
     4381 cactaaagga taaaactgat aatatcctct tatgcggata aatttgctgt ggacgtatga
     4441 catgatgagc tttcagaaga tctatagccc aacgcaattg gcgaatgcaa tgaaactggt
     4501 tcgccagcaa aatggctgga cgcagagcga gctggcgaaa aaaattggca ttaagcaggc
     4561 gacaatttcc aatttcgaaa acaaccctga caataccacg ctcacgacat tttttaagat
     4621 tttacagtcg cttgaactct caatgacact atgcgacacg aaaaatgcct cgccagaatc
     4681 aacagaacag caagatctgg agtggtaatg cctaaacttg tcacttggat gaacaaccag
     4741 cgggtaggcg agttaacgaa gttagccaac ggcgcgcaca cctttaagta tgcaccggag
     4801 tggttagcca gccgttatgc cagaccgttg tcactttcgc tgccattgca gagggggaat
     4861 atcacctcag atgccgtatt taacttcttc gataacctgt tacccgatag cccgattgta
     4921 cgtgaccgga tcgttaaacg ttatcatgct aaatccagac aaccgtttga tttattgtca
     4981 gaaatagggc gagacagcgt tggtgccgta acgttattac ccgaaaatga aaccataacg
     5041 cgcccgataa tggcatggga aaagcttact gaagccaggc ttgaagaagt attaacggct
     5101 tataaagcag atatccctct gggcatgatt agagaagaaa atgactttcg catctcggtt
     5161 gctggcgcgc aggagaagac agcgctgctt agaataggca atgactggtg cattccgaaa
     5221 ggaataacgc cgacgacgca catcattaaa ttaccgattg gcgaaatcag acagcccaat
     5281 gcgacgctcg atctcagcca aagcgttgat aatgagtatt actgtctgct gctggcgaaa
     5341 gaacttgggt tgaatgttcc ggacgcagaa atcattaaag cgggaaatgt gcgcgcgtta
     5401 gcggtcgaac gttttgacag gcgttggaat actgagcgaa cggttttact tcgcttgcca
     5461 caggaggata tgtgtcagac attcggttta ccttcatctg tgaaatatga atcagatgga
     5521 ggcccaggca tcgcgcggat catggcgttt ttgatggggt ccagcgaggc gcttcgcgat
     5581 cgttatgatt ttatgaaatt ccaggtcttc cagtggttga ttggcgcaac ggatggccat
     5641 gcaaaaaact tctccgtatt tattcaggct ggcggcagtt atcgactcac gccattttac
     5701 gacatcattt cagcatttcc ggtccttggc ggtacgggaa tacacatcag cgatctcaaa
     5761 ctggcaatgg ggcttaacgc atccaaaggc aaaaaaacgg caatcgataa aatttatccg
     5821 cgacattttt tggcgacagc aaaggtgctg agattcccgg aagtgcagat gcatgaaatc
     5881 ctaagtgact ttgccagaat gattccggca gcactggata acgtgaagac ttcattaccg
     5941 acagattttc cggagaacgt ggtgacggca gttgaaacca atgtgttgag gttgcatgga
     6001 cggttaagcc gagaatacgg tagtaagtga gatatggggt ttggtcgttg ttaatgagca
     6061 cgacaatcat gaccgccaac gactggattc ttccttggtt tatactggtg cgcataatgt
     6121 atattatgtt aaattatgtc tgagtgatgc gaagtgcttt tctggtagtc gttattcgtt
     6181 cagggttcaa gaacttaagc ctctcctctt tcataattgg cctgtgcatg gagaaatagc
     6241 ctggttattc cctgccccat gctaagagcg cagtgtctgt cgcatagaca ctgcatacga
     6301 aaacaccaga gggtgctgtt acttatttaa ggacggatca agcgggaatg agtccactcc
     6361 ggtatcagaa atggctacgt ctcaatgaag tcaggcgacc gatgctgaat gaacattacg
     6421 atgtcaccac agcagcctat gctgtcggtt acgaaagcta tcccatttca gtcgggaata
     6481 ttcgcggatg tttggagagt cacccaagag agatattacc aggttaagaa agtctgtcgg
     6541 tcagctttaa taaaccctgc acttatctgt ttagagcaat tcggtgttag tttcagcaag
     6601 caaacattaa ccatagctaa tgatttatag ccatattaac cattggtagc ccttgacctt
     6661 tcctgacact tcccttgttc gcaataatat tctgtttagt cacggtttat aaataacatg
     6721 cataatgaaa tcgtcatgtt atttagcgac ataaaatgtt tttgcaaata ctctcttcaa
     6781 caagtgcaca ataaacttgt tgtgattagg tgtccttgtg atttaaagat attatcgttc
     6841 ttcgtgaaat gtaataaccc tggaaactat attctcaggg caacaaccaa tcaagaatga
     6901 ttcttgtttt taagcaataa cagaatgact taattgttaa ccctctttat tcagctactt
     6961 aaatataaat tttggagaat tatgaaactc aaacatgttg gtatgattgt cgtttctgtg
     7021 ttggcgatgt cgtctgctgc ggtaagcgca gccgagggtg atgaatcagt aacgaccact
     7081 gttaatggcg gtgttattca ttttaaaggt gaagtggtaa atgccgcttg tgcaattgat
     7141 tcagaatcaa tgaaccaaac ggttgagctg ggtcaggttc gttcttctcg
//
LOCUS       DAZFWL020000195         7149 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFWL020000195 DAZFWL020000000
VERSION     DAZFWL020000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.195"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            43..1095
                     /locus_tag="ACHKNP_RS22790"
                     /old_locus_tag="pgaptmp_004558"
     CDS             43..1095
                     /locus_tag="ACHKNP_RS22790"
                     /old_locus_tag="pgaptmp_004558"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HGU4706586.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1171..2604
                     /locus_tag="ACHKNP_RS22795"
                     /old_locus_tag="pgaptmp_004559"
     CDS             1171..2604
                     /locus_tag="ACHKNP_RS22795"
                     /old_locus_tag="pgaptmp_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HGU4706587.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2787..5048
                     /locus_tag="ACHKNP_RS22800"
                     /old_locus_tag="pgaptmp_004560"
     CDS             2787..5048
                     /locus_tag="ACHKNP_RS22800"
                     /old_locus_tag="pgaptmp_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HGU4706588.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5282..6565)
                     /locus_tag="ACHKNP_RS22805"
                     /old_locus_tag="pgaptmp_004561"
     CDS             complement(5282..6565)
                     /locus_tag="ACHKNP_RS22805"
                     /old_locus_tag="pgaptmp_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HGU4706589.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(6700..>7149)
                     /locus_tag="ACHKNP_RS22810"
                     /old_locus_tag="pgaptmp_004562"
     CDS             complement(6700..>7149)
                     /locus_tag="ACHKNP_RS22810"
                     /old_locus_tag="pgaptmp_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4706590.1"
                     /translation="VTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDAL
                     GISMKETLDKCKEILGGETIIASTHREPLSSGTVSRYFMRARKASGLSFEGDPPTFHE
                     LRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1722 a         1711 c         1847 g         1869 t
ORIGIN      
        1 ccatatcgca ataagttatg tttttaaatt gagggcatta ttatgaaaaa aataccctgc
       61 gtgatgatgc gaggtggaac ctcgaggggc gcgttcctgt tagcggaaca tttacccgaa
      121 gatcaaacgc agcgcgataa aatattgatg gcaattatgg gttccggtaa cgatctggaa
      181 attgacggta ttggcggcgg taatccactg accagtaaag tcgccattat tagccgttcc
      241 agcgatccgc gtgctgatgt tgattatctg tttgctcaag ttatcgtcca tgagcaacgt
      301 gtcgatacca cgcctaactg cggcaatatg ctttctggcg ttggggcatt cgccattgaa
      361 aatggtttga ttgcagcgac atcaccggtc acccgtgtgc gtatccgcaa cgtcaatacg
      421 ggtacgttca tcgaagctga tgtgcaaacg ccaaatggtg ttgtcgagta cgagggtagt
      481 gccagaattg acggcgtacc gggtactgcc gcaccggttg cgctcacttt cctgaatgcc
      541 gctggaacca aaaccggaaa agttttcccg actgataatc agattgatta ttttgacgat
      601 gtcccggtga cctgtatcga tatggcgatg ccagtcgtca ttattccggc tgaatatctg
      661 ggaaaaacag gttatgaatt accggcggaa ctggatgccg acaaagcatt attagcccgc
      721 attgaatcta tccgtctaca agcgggtaaa gcaatgggct taggtgatgt cagtaatatg
      781 gttatcccta aacctgtgct tatttctcca gcgcagaaag gcggggcaat taatgtgcgt
      841 tattttatgc cgcattcttg ccatcgcgcg ctggcgataa ccggtgctat tgctatttcc
      901 agtagttgtg cattggaagg caccgtcacc cgacaaatcg tcccttctgt aggatacggc
      961 aatatcaata ttgaacaccc cagtggtgcg ctcgacgttc atttaagtaa tgaaggtcag
     1021 gatgccacga cgttacgcgc atctgttatt cggacgacca gaaaaatatt ttccggtgaa
     1081 gtttatcttc cctgaaaaaa ttcgttgtca agataaggat aatcaataaa ggacttctgt
     1141 atgagtcata cagaaagaac aggattttaa atgaataaga aatcgttatg gaagctaatt
     1201 ctgatattag cgatcccatg tattattggt tttatgccag ctccggcagg attaagcgaa
     1261 ctggcgtggg tgctttttgg tatttacctg gcggccattg tggggctggt tatcaagcct
     1321 ttcccggaac ctgtcgtact gttaattgcc gttgctgcct ccatggtggt ggtcggtaac
     1381 ttatccgacg gtgcgtttaa gaccaccgcc gtattaagcg gttactcttc aggtactacc
     1441 tggctggtgt tctcggcgtt taccttaagc gccgcatttg tgaccaccgg tttaggtaaa
     1501 cgtattgcct atctgctgat tggtaaaatc ggtaacacca cgctgggtct gggttacgtt
     1561 acggtattcc tcgatctggt actggctccg gcaacaccgt ctaacaccgc gcgtgcgggc
     1621 ggtattgtgt taccgatcat caacagcgtg gcggtggctt tggggtccga accggaaaaa
     1681 agtccgcgtc gtgtcggaca ttacctgatg atgtccattt acatggtcac caaaaccacc
     1741 agctatatgt tctttaccgc aatggcgggg aacattctgg cgctgaaaat gatcaacgac
     1801 attctgcacc tgcaaattag ctggggtgga tgggcactgg ccgccggatt gccgggcatc
     1861 attatgctgc tggtcacccc actggtgatt tataccatgt atccgccaga aattaagaag
     1921 gtggataata aaaccatcgc taaagcgggc cttgccgaac tgggaccgat gaaaatccgc
     1981 gaaaaaatgc tgctcggtgt ctttgtgctg gcgctactgg gctggatttt cagtaagtct
     2041 ctgggggttg atgaatccac tgtggcaatc gttgttatgg cgaccatgct gctgctgggt
     2101 atcgttacct gggaagacgt ggttaaaaat aaaggcggct ggaatacctt aatctggtac
     2161 ggcggtatta tcggattaag ctccttatta tcgaaagtta aattcttcga atggttagct
     2221 gaagtcttta aaaataacct ggcatttgat ggtcacggta acgttgcttt cttcgttatt
     2281 attttcctca gcattatcgt gcgttatttc ttcgcttccg gtagtgccta tatcgttgct
     2341 atgttaccgg tatttgccat gctggcgaac gtctccggcg caccgttaat gttaaccgcg
     2401 ctggcactgt tgttctccaa ctcctatggc ggcatggtta ctcactatgg cggcgcggca
     2461 ggtccggtca tctttggcgt gggttataac gatattaaat cctggtggtt ggtcggtgcg
     2521 gtactgacga tattaacctt cctggtgcat atcaccctcg gcgtgtggtg gtggaatatg
     2581 ctgatcggct ggaacatgct gtaaatattc tcgtcatact tcaagttgca tgtgctgcgt
     2641 ctgcgttcgc tcaccccagt cacttactta tgtaagctcc tggggattca ctcgcttgtc
     2701 gccttcctgc aactcgaatt atttagagta tatccattta ttatctttct gcgcacttca
     2761 cggtgcgcag atatctggag catttgatga tcaagttatc tgaaaaaggc gtgtttctcg
     2821 ccagtaataa cgaaataatt gccgaagaac atttcaccgg cgaaattaaa aaagaagaag
     2881 ccaaaaaagg cactattgcc tggtctattc tctcttcgca taatacgtcc ggaaatatgg
     2941 ataaacttaa aattaagttt gattcattag cctctcacga tattaccttt gttggtattg
     3001 tacagaccgc taaagcgtcc ggtatggaac gtttcccgct gccgtatgtg ctgaccaact
     3061 gccataactc actctgcgcc gtcggcggca ctattaacgg tgatgaccat gtttttggtt
     3121 tatcggcggc ccagcgttat ggcggtattt ttgtgcctcc gcatattgcg gtcatccatc
     3181 aatatatgcg tgagatgatg gcaggcggcg gcaaaatgat cctcgggtca gacagccaca
     3241 cccgttacgg tgcattaggg acaatggcag tcggtgaggg cggcggtgag ttggtaaaac
     3301 agctgcttaa tgacacctgg gatatcgact atccgggcgt ggttgcggtg catctgaccg
     3361 gaaaacctgc gccgtatgtg gggccacagg atgtggcgct ggctatcatt ggcgcggtgt
     3421 tcaaaaacgg ttacgtcaaa aacaaagtca tggagttcgt tggaccgggc gttagcgcgc
     3481 tctctaccga tttccgtaac agcgttgacg tgatgaccac ggaaaccacc tgtttaagct
     3541 ccgtctggca aactgatgaa gaagtgcata actggctggc gctgcacggt cgcggccagg
     3601 attactgcca gcttaaccct caaccgatgg cgtactacga tggctgcatc agcgttgatt
     3661 taagcgccat caaaccaatg attgcgctgc cgttccaccc gagcaacgtg tatgaaatcg
     3721 acacactgaa ccagaacctg accgacattc tgcgtgagat tgaaattgag tccgaacgcg
     3781 tggcgcacgg taaagccaaa ctctcgctgc tggataaagt ggaaaatggt cgcctgaaag
     3841 tgcagcaggg gattatcgcg ggctgttctg gcggtaacta cgaaaacgtc atcgcggcgg
     3901 cgaatgcact gcgcggtcaa tcctgtggca atgacacctt ctcgctggca gtttacccgt
     3961 catcacagcc ggtgtttatg gatctcgcca aaaaaggtgt ggtagcagat ttgattggcg
     4021 caggcgcaat catcagaacc gcgttctgcg gcccatgctt tggcgcgggc gatacgccaa
     4081 tcaacaacgg tttgagtatt cgccacacca cgcgtaactt cccgaaccgc gaaggctcta
     4141 agccagctaa tgggcagatg tcagcggtgg cgttgatgga cgctcgttct atcgctgcga
     4201 ctgcggcaaa cggtggctat ttaacctctg ccagcgaact tgattgctgg gacaacgtgc
     4261 cggagtacgc cttcgatgta acgccgtata aaaaccgtgt ttatcagggc tttgtgaaag
     4321 gggcaactca gcaaccgctg atttacggac cgaacattaa agactggccg gaattgggtg
     4381 cgctgactga caatatcgtc ctgaaagtgt gctcgaagat cctcgacgaa gtgaccacca
     4441 ccgacgaact gattccttcc ggtgaaacct cttcttatcg ttcaaatccg attggtctgg
     4501 cggagtttac cctgtcacgc cgcgatcccg gttatgttgg cagaagtaaa gcgactgctg
     4561 agctggaaaa tcagcgtctg gcggggaatg tcagcgagct gacagaggtg tttgcgcgca
     4621 ttaagcagat tgctggtcag gagcatattg atccgctgca aactgaaatt ggcagcatgg
     4681 tatatgcggt gaaaccaggc gatggttctg cgcgtgaaca ggcggcgagc tgccagcgtg
     4741 tgattggcgg tctggcgaat attgccgaag agtacgcgac taaacgctac cgttctaacg
     4801 tcatcaactg ggggatgtta ccgctgcaga tggcggaagt gccaaccttt gaagtggggg
     4861 attacattta catccctggc attaaagcgg cgctggataa tccgggtacg acgtttaaag
     4921 gttatgtgat ccatgaagat gcgccggtaa cggaaattac gctctatatg gaaagtctga
     4981 ctgctgaaga gcgcgagatt atcaaggcgg gtagtttgat taacttcaat aaaaaccgtc
     5041 agatgtaaaa agcgccatgt gaatgtaggt cgcattcggc acttattgtc ggatgcgatg
     5101 cttgcgcatc ttatccgacc tacgaatcgc atcgaatctg taggccagat aaggcatttt
     5161 cgcagcatcc ggcacttatt gtcggatgcg atgcttgcgc atcttatccg acctacaaat
     5221 cgcatcgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcaaata gttaattgct
     5281 cttacttctt cgcctctgca accactttac tacccacgcc gcggttattg tattcccaca
     5341 tgcggttgta gttagtgtca ttcagattgc gctgtatttc gtcgttatca tctacgctgc
     5401 cggtattacc cgcaaacgga cgattagaga tcaccgcatc ggcccacggt ttagccgtgt
     5461 taaaaccttc gttgatggcg ctatcacgga tcaccacctg accgttggta ttggcatcaa
     5521 catccagcga gcggcccagt tgcgccacac catcaccgaa agcattgaaa cggctgttta
     5581 cggcgaggaa accgtagtaa atgttggaca gcgtagccgg tgcaaacaca tacgcttctt
     5641 gctgagtacg tgagttcacc acgcggaatt cggtgttatc gaacaccact gcgccgcgac
     5701 cagaaacgat atccacatcc ccttcaatgt agctgttggt caccagcgta cgcggctgac
     5761 gattcgtttc cagacggttc tgcacaccgc tgttggtgac aaagaaggtg ttctgacgac
     5821 cgagaatgtt aacgttgtta atctgtacct ggtcaccatc agtacgcagt gccaccgccg
     5881 gatggttacc tgcatctacg ctatcgccca gcgtgttttc gatggtcaga ttttgcagtt
     5941 gcaggccatt gttttgtgac cagaagaccg cagagcagag aacaccgata ctgtcgctgc
     6001 gtttgctctg gcagctatcg tacatatacc acgctggttt acctggcata tatttgccgc
     6061 gcgggttgac gtcgtgacgc cagtcggcag ggctcatgcc accatcaagg gaaagcccaa
     6121 tcttcacatc aatcggtttt tcacctgtac cgtacagagt aattccaccc ggagcggcag
     6181 ggacatatac cgttccctga tactcaccag gcatcacggc aatatactgg cgcttgttgg
     6241 tacgcttgat aattgccgca tctaccgccg cctgaatcgt ggtatgcgtt acaccttgag
     6301 tgcccgccgg gccgacaaca aagtcaggtt gcgcaggcag ggtaatcggg gaaggattcc
     6361 acgctgctgc acctggtgtc agggatgcaa aatagtgttg agcatcgaaa ttctgcgctt
     6421 cttttgccga cagaatcggg cgagaagagg taccaggcgc ggtttgatca gaaggacgtt
     6481 gatcgggcgg ggttgagcta caggcggtca gcgtcacgcc aaaagccaat gccagcgcca
     6541 gacgggaaac tgaaaatgtg ttcacaggtt gctccgggct atgaaataga aaaatgaatc
     6601 cgttgaagcc tgctttttta tactaagttg gcattataaa aaagcattgc ttatcaattt
     6661 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgattt caattttgtc
     6721 ccactccctg cctctgtcat cacgatactg tgatgccatg gtgtccgact tatgcccgag
     6781 aagatgttga gcaaacttat cgcttatctg cttctcatag agtcttgcag acaaactgcg
     6841 caactcgtga aaggtcggcg gatccccttc gaaggaaaga cctgatgctt ttcgtgcgcg
     6901 cataaaatac cttgatactg tgccggatga aagcggttca cgatgagtag atgcaattat
     6961 ggtttctccg ccaagaatct ctttgcattt atcaagtgtt tccttcattg atatcccgag
     7021 agcatcaaca tgcaatgttg tagggatggc aatttttacg cctgttttgc tttgctcgac
     7081 ataaagatat ccatctacga tatcagacca cttcatttcg cataaatcac caactcgctg
     7141 cccggtaac
//
LOCUS       DAZFWL020000196         7139 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFWL020000196 DAZFWL020000000
VERSION     DAZFWL020000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.196"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..296)
                     /locus_tag="ACHKNP_RS22815"
                     /old_locus_tag="pgaptmp_004563"
     CDS             complement(<1..296)
                     /locus_tag="ACHKNP_RS22815"
                     /old_locus_tag="pgaptmp_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4706591.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRV"
     gene            complement(293..2905)
                     /locus_tag="ACHKNP_RS22820"
                     /old_locus_tag="pgaptmp_004564"
     CDS             complement(293..2905)
                     /locus_tag="ACHKNP_RS22820"
                     /old_locus_tag="pgaptmp_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4706592.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVHRHFSSLEADARKTASTV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISD
                     SFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAA
                     GGGVAVLAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAGVISDEAGGGIR
                     QAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALAS
                     LNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIW
                     DDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETT
                     KNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEMGYINRSRATGV
                     SKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAELRTLEKHSGA
                     NEKISRQRRDLWKAESQYAVLKEAATKRQLSWQEKSLLAHEKETLEYKRQLADLGDKV
                     EHQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPQA
                     LARVTGALKQTWADEDMLRGDWLAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQN
                     MAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTA
                     IEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEG
                     GYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDELR
                     LQLRDGGMLSGSGR"
     gene            complement(2886..3299)
                     /locus_tag="ACHKNP_RS22825"
                     /old_locus_tag="pgaptmp_004565"
     CDS             complement(2886..3299)
                     /locus_tag="ACHKNP_RS22825"
                     /old_locus_tag="pgaptmp_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4706593.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            complement(3326..3748)
                     /locus_tag="ACHKNP_RS22830"
                     /old_locus_tag="pgaptmp_004566"
     CDS             complement(3326..3748)
                     /locus_tag="ACHKNP_RS22830"
                     /old_locus_tag="pgaptmp_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4706594.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEQTGKAEATEPVTSKKHSKAS"
     gene            complement(3762..4514)
                     /locus_tag="ACHKNP_RS22835"
                     /old_locus_tag="pgaptmp_004567"
     CDS             complement(3762..4514)
                     /locus_tag="ACHKNP_RS22835"
                     /old_locus_tag="pgaptmp_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4706595.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VTAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(4522..4917)
                     /locus_tag="ACHKNP_RS22840"
                     /old_locus_tag="pgaptmp_004568"
     CDS             complement(4522..4917)
                     /locus_tag="ACHKNP_RS22840"
                     /old_locus_tag="pgaptmp_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4706596.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(4914..5492)
                     /locus_tag="ACHKNP_RS22845"
                     /old_locus_tag="pgaptmp_004569"
     CDS             complement(4914..5492)
                     /locus_tag="ACHKNP_RS22845"
                     /old_locus_tag="pgaptmp_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4706597.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(5504..5857)
                     /locus_tag="ACHKNP_RS22850"
                     /old_locus_tag="pgaptmp_004570"
     CDS             complement(5504..5857)
                     /locus_tag="ACHKNP_RS22850"
                     /old_locus_tag="pgaptmp_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4706598.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(5869..6264)
                     /locus_tag="ACHKNP_RS22855"
                     /old_locus_tag="pgaptmp_004571"
     CDS             complement(5869..6264)
                     /locus_tag="ACHKNP_RS22855"
                     /old_locus_tag="pgaptmp_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4706599.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(6306..>7139)
                     /locus_tag="ACHKNP_RS22860"
                     /old_locus_tag="pgaptmp_004572"
     CDS             complement(6306..>7139)
                     /locus_tag="ACHKNP_RS22860"
                     /old_locus_tag="pgaptmp_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4706600.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAANNITQSG
                     GTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVRDKLDTRRGS
                     HSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDEFVSVQL
                     A"
BASE COUNT         1438 a         2327 c         1705 g         1669 t
ORIGIN      
        1 tcaacgcgca acatcccgac ccgcgcagac cacccggcac aggtcacctt tatctgccgg
       61 tatgcatagg gtggcttcca caaaaatgcc ttccagcctc cgtgctctgc caggaacgct
      121 tccagatgcc gggcctcctc ccgggtcacg gaaagcgtca cacggtatgt tttcaggtca
      181 gcattcagcc ctgccgccat acgctgtgag tacccgtcac caaaacgcac ttcacgcacc
      241 gatggctgcg agttcacctc catatccggc ttcactttcc agcgaaatgt tttcatcgcc
      301 cgctccctga taacataccg ccatcacgca actgcagccg gagctcatcc tgcgccccct
      361 tgcgggccat gtcatacacc gccttcatca gctgcggccc cgcctgtccg ttgataccgt
      421 cgttctgaat caccacgtga ttgttctgat taaaattaat accttccgcc cgccgcatct
      481 gcgccggact tccggcacca cccacataac caccttccgc atagccgcgc atcagacggt
      541 aaagattccc cacacctatc cggctggttg cctctttcgt gaaaacaaac tccccgcggt
      601 gaactatccc cgcaggctca tatttgccgc ccgtccccgt aaatcctccg gtcgcgaaat
      661 ggaagttcgc cgccgcagcc tcaatggccg tccccgagga agcagatgca ccaccaccga
      721 aagcaccgcc aatggcgctg ccgatacgcc cgacaatgcc caccatggcc tgtttaagca
      781 ggatttctgt catcatggac agcaccgaac gggtgaatcc ccgccagtct gcctctgcac
      841 cggtcagcat cgccgccata ttctgtgcaa taccgtcaaa ggtctgcgtg gcagcacttt
      901 taacctgcga aaaactgtcc gtcgcacttt ccgcccactc cccccagccg gacttcagcc
      961 cggccagcca gtcaccgcgc agcatgtctt catccgccca tgtctgtttc agtgccccag
     1021 tgacccgggc cagcgcctgc ggattatcac cgtacacgtc ccgaagacgc tgcgcttcag
     1081 actcccgctg cgcctgacgg tcagtgagac cgcgggcttt tgcgctgatg gcggcctgct
     1141 tcgcgctctg ctgctcttca aaccgcaccg cctgctgtgc cagctcattc aggcgtttct
     1201 ggtgttcaac cttgtcgccc aggtcagcca gctggcgttt gtactccagc gtctctttct
     1261 catgggccag cagggatttt tcctgccagg ataactgccg tttcgtggca gcctctttca
     1321 ggaccgcata ctgactttcc gccttccata aatcacggcg ctgccgactg attttctcat
     1381 tcgcaccgct gtgtttttcc agcgtcctga gctcagcttc aagggcaagc agagcctctc
     1441 ttgcctgctc ctcttccctc tccccggcag agcgcgtttt cggtgatgta tgctttttac
     1501 ctgtcagctc ttcagccaga cggctgacgg cttcctgctg cccaggacct ttgctgacgc
     1561 ctgttgcacg cgagcggttg atgtacccca tttccccctg gcgtatacgc gcatcccgtt
     1621 ccgcaatgga ttttctcagc gccagttcat cgcgttttgt tttctcaata aatacgcggt
     1681 tctcttctgc cagttcacca aacaacgcac caacgccggg cacattcttt gtcgtttccc
     1741 aggctgactg aataaattca gccagcgcca gatccccctg cacaagcagc agcttcactt
     1801 gttcaacggt tccggccacc acgtcagtga tcagactgag tgcccccagt gtatgatcac
     1861 ctatccatgc ccatgcgtca gaagtccagg ttttaacatc gtcccagatt ttttccaccg
     1921 gcgtggccgc tttatcaagt tgctccagac gtgcattcat gacatccgca aacagggaca
     1981 tcgcctccgt caccgcagcc tgtttacctt tcgtgcgctc aagctcatca atatggcgta
     2041 actgggaaac gctcaggaag ttatactgct gattcaggga ggccagcgcc ttcaccggat
     2101 ctgctgcaat cccttcaaag gctttttcca ccttcccggc atcgtccccc accgtctgca
     2161 gccatctctg agaggtttcc cccatgatcc gtagctgccc ggcggtatat ttcccgcttt
     2221 ctgccagacg ggccagattt tctgccgcct gtctgatacc accaccggct tcatcgctga
     2281 tcaccccggc cattttccac aattctgccg ttgtggtggc agccgcccct ccggtcagga
     2341 tcagtgaacg caataaggcc cggtcagcct gctctgcctg ccaggcggcg gcagcaagcg
     2401 cggccagtac ggcaaccccg ccacctgccg ccacacgggc caccgacata aatcgtccca
     2461 gctcaccggc attccgggca ttttcagcca gtgcatttgc cgtatctgac agcgactcct
     2521 ctgatgattc agaggcatcc ctgatcccga gaagttcctc cttcagcagg gtaagcaggc
     2581 tgagcggtcc accgaatgaa tcgctgatct gccccccctg ctgcagcatg ataaggaagg
     2641 gattctgacc accggcaagc tgagtgacaa tatccgtgaa ctgtgcgggc agtgtgcgca
     2701 tggcagcctt atactgtccg actgatatcc cggctttttg tgcagccagc gcctgtcggc
     2761 tcagcccctg ttcaacagta ctggcggttt ttctggcatc cgcctccaga ctggaaaaat
     2821 gacggtgtac ccgggccatc tgttcatcaa accgggccgc atccagactc aaatcaataa
     2881 ccagatcacc cgctggctgg gacatatctc acacctccgg aaatccccgc tgaagccatc
     2941 attaatgcaa catcatcctc gctgacatcc accacatccg cagaaggtga aatatcgcgc
     3001 cctccgtccc ctccgaaccg gacgcctccg gcaactcctg tcgctttctg catcagcatt
     3061 tcttcctcgt ccggcatctc cgtctgctct tcctcacacg ctggagcaag cagactgaaa
     3121 tccgccggat gcatatccgg atcgccaaaa aacaggctga gtacggcgta catcagcccg
     3181 gaaaaatgag cgtccagttg ggtatcctga aaataatgcg tgcagtaaaa acgtcgccag
     3241 tcggcatatt cggtggatgt catcccggca agcatggcgc gccagtcggg tctccccatc
     3301 tctcgcgcca gtttcaggac aaagttcagc tcgccttcga atgctttttt gatgttaccg
     3361 gctcagtcgc ttctgctttc ccggtttgtt caggatcggc atcgtgccgg ttatccagca
     3421 tacctgaaag ataaagcacc cggttcgttg cctgattcag tgcatcagca ggccatccca
     3481 gcatcacttc acggcggatc tgctgcatct ctgtctccgg agaggccaga gtgcctttga
     3541 gggaatggga atgccatagc gacatcgcca caagcaggga tgccgtttcc agatatcgct
     3601 ggttaatgtg cacgacatca tgcttcgttg tctcctgttg ttctgcgtct gaaacaaact
     3661 ttatataatc aaaccgctgc agcgcagaca attcggaaag cgtgacggac acaccgttat
     3721 attcaaattg ttctgttttc agaaacatgt attacctccg tttaccctgc agcgcccgct
     3781 tcagtaacgg tgacttcagc cactgcggcg aactgaccat ttccgctcac cacagggatc
     3841 tgcaccttac ctgtcgccac gccgtttacc gtaattgtca tatctttcac actaatggtg
     3901 gctttcgacg gatcggcgga aaccgctctg aacgtcttgt cggttgcact ttccggctca
     3961 aaagaaaccg tcagggtggt tgttttccct tttgccaccg taccggatgt cggcgtcacc
     4021 ttaatcgcag tgaccggcgt aattttgctg cgttcttccg ctacagaagg tttgcccacg
     4081 ttggtcactt tcaccgtgcg ggtgatcact tctttcgccg tcacggcctt accgatactg
     4141 ctgacccagc cacggaacac atccaccgtg ccatttggga aacggatttt ataggcccgc
     4201 acatccccgc tttcaaacca gcctataagc cctttctgac cttcttctcc cggtttccag
     4261 gccagcgtaa aactggtatc tcctgcagac ttctgcccct gcccggtcgc ggtccagtcc
     4321 gcgtcttcat catccaggta gttatcatcg tagggttctg ccgtcatctc gcccggcgtc
     4381 agatccttca ccttagccag tcgctgccag tcatcgtctg acaacgggtt tgcataagca
     4441 tcacccttgc cgttgtaaac ccacagagtg gtaccggcac cttttaccgg ctcaagggga
     4501 tttggtgttg ccatatcgtc ctcacatctc gtatgtaatg gaataagtca gatctgcaga
     4561 actccataac gccatatcgt catcacgacg atactcatag ccctgcgtaa ccatcgtggt
     4621 aatcagtcct gccagtgccg ggatatcgct catcaccgga taaatccggg actccatcca
     4681 cgcatccagc tctgaatccg gcacctgagc aggcaggaaa acttcaatat gcagctccgc
     4741 ctgccaggta tcgctgtcca gctcttcgcc cgtgtattca gcgccggtga gataaacggc
     4801 aactgccgga aaatccgcct catcaaaaac agcggggcga ccatcaaaaa gcgtcgcccc
     4861 ggtgtcatgc ttctccagtg catccagtac ggctgcacgg agttcagtat gtttcatcgc
     4921 tttattacca ttctcagttg atgctgcagc gcatagccca gctctttcgg aagacgttca
     4981 cgccgtatcc gttcaatatt ctgtttaaac gccgtggtaa gcggcaccgc catcgggatt
     5041 ttcaccacat caatggggta acggtttttc ccggccacac gctgcatgac atgccaccgc
     5101 ccatttttca gttgctgaat aaacgcgccg ggaatacgac ggtttcccac cacaagcacg
     5161 ctgccgccac ctttcaggga tgaacgctgc ccctttttac gacgcctgcg tcgggacagg
     5221 acaacccgcg cattccccag cttgattacg ggcaaatccc cccggttaac cttgattctg
     5281 gcctgcggat ttttaaccgt ggcccttttc agcctggccc tttcctttac cagtttccgg
     5341 cgtacctttg tctcacgggc aacctgtgac gcagactgcg atatcgcgga tgaagcaacg
     5401 cggttaatgg ccattgctga cgcaccgggc accgccgttt tgctgatacg gctgaggttt
     5461 tcaacggcct gctcaagacc ttttatggcc atacatcccc ctttcagcgg cgacggttaa
     5521 cggcaggcgg cacgccacgc ccaagccaga gatgacagct tccgccatca tccggcgaaa
     5581 tccggtctat ccagaagttt tcctcaccga tggtcagcgt gtctccacgc cgcagctgcc
     5641 gcacatcatc agtccggaca aacagggacg ggctggagcc ttcaacgcgc acgccctgtc
     5701 cggcatagct gatattttca gggtcatcaa aaacaccacg tattactgcg ccggactgct
     5761 caccggatgt catggtggct gacgttccca tgtacccgcg tatcgtttca tcggcgcggg
     5821 caatggcagc atcgaacagg ttatcgaaat cagccacagc gcctcccgtt attgcattct
     5881 ggccaggcca cgttctgtca tttcggctgc cacaccggca gagacacgaa acgccgttcc
     5941 cggcagcaca aatgccacag gttcatcccg cgtggcgtga agtgcatcgg tatgcagcgt
     6001 caccagtgcc acaaccgtga ccagagcagc cgtatcagtc acggtatccg tctgtgctga
     6061 taccacctca ttttcatgtc cggtcagcgc attttccggg ctgacagacg tgtcctgacc
     6121 ggctgcgtca tccgtgtcat caagctcctc ttccagctct gccacacgga gcgccagttc
     6181 ttctttcgtc cccgtcaggc tgacatcacg gttcagttgt tcacccagcg agcggagacg
     6241 ggcaatcagt tcatctttcg tcatggactc atccacagag aaacaatggc cccgaagggc
     6301 catgattacg ccagttgtac agacacgaac tcatcagggt cagccagcag catcagcggt
     6361 gctgactgaa tcatggtgaa ctctcgcgcc ggatcgccgg tagtcaccca gtttttcggg
     6421 taacgggcag aggcgttaat gccttcgcgc tgtgcgtccg catcctgaat acagccatag
     6481 gtacgcagac cgcgtgcctg agtgttcccc agcaccatcg tgttgtccgg caggaagttc
     6541 tttttgacgc cgttttccac gtactgtccg gaatacacga cgatggccac atcgccatac
     6601 attcccttat aagacaccgc tttgcccagg tcttttaccg ctgtctccag ctcggaatga
     6661 gagccgcgac gggtatccag cttatccctg acggctttga aggaacggaa cagcgcccat
     6721 cctttcggat caaacacgat gatattcacc acgccgctgg cgttcagcgc gtaggcttcg
     6781 atatcgtcgg tcgggtcata cgtggacttg tcacgcttgc tccactccgt gccaccggac
     6841 tgtgtgatgt tgtttgccgc actgcggccc atatccacct caaccggatc aaaggcttca
     6901 ccggtcatgg tgtatttgcc cttaagcacg gcagaaactg cctgcatctc ttcgacctgg
     6961 gcaatggcca gctcttcgtc acgcatgttc tgcatgatga tgcgacggcg gcggtaagcc
     7021 gggtccgcca gattctgcgg atcttcatcc ggcaggcgac gcagggtcat ctgcggattc
     7081 acctcatgct tcggcttgac atatcccggc gtaaattcag aggtggagcc gccacggga
//
LOCUS       DAZFWL020000197         7082 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFWL020000197 DAZFWL020000000
VERSION     DAZFWL020000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.197"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..184
                     /locus_tag="ACHKNP_RS22865"
                     /old_locus_tag="pgaptmp_004573"
     CDS             <1..184
                     /locus_tag="ACHKNP_RS22865"
                     /old_locus_tag="pgaptmp_004573"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706601.1"
                     /translation="SRNISYALKLNYKGGQTPSAHQERPGCFPGVFRQPLTPIMGSIP
                     TFIPEMNTGFNLLNKK"
     gene            308..1435
                     /locus_tag="ACHKNP_RS22870"
                     /old_locus_tag="pgaptmp_004574"
     CDS             308..1435
                     /locus_tag="ACHKNP_RS22870"
                     /old_locus_tag="pgaptmp_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HGU4706602.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            1441..2232
                     /locus_tag="ACHKNP_RS22875"
                     /old_locus_tag="pgaptmp_004575"
     CDS             1441..2232
                     /locus_tag="ACHKNP_RS22875"
                     /old_locus_tag="pgaptmp_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HGU4706603.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(2268..3193)
                     /locus_tag="ACHKNP_RS22880"
                     /old_locus_tag="pgaptmp_004576"
                     /pseudo
     CDS             complement(2268..3193)
                     /locus_tag="ACHKNP_RS22880"
                     /old_locus_tag="pgaptmp_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(3338..4687)
                     /gene="lysC"
                     /locus_tag="ACHKNP_RS22885"
                     /old_locus_tag="pgaptmp_004577"
     CDS             complement(3338..4687)
                     /gene="lysC"
                     /locus_tag="ACHKNP_RS22885"
                     /old_locus_tag="pgaptmp_004577"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HGU4706604.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     regulatory      complement(4779..4975)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            5212..6861
                     /gene="pgi"
                     /locus_tag="ACHKNP_RS22890"
                     /old_locus_tag="pgaptmp_004578"
     CDS             5212..6861
                     /gene="pgi"
                     /locus_tag="ACHKNP_RS22890"
                     /old_locus_tag="pgaptmp_004578"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGU4706605.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALVGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDGKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1797 a         1701 c         1836 g         1748 t
ORIGIN      
        1 ttctcgcaat atctcgtacg cgttgaaact gaattacaaa gggggtcaaa ccccgtcggc
       61 acaccaggaa cggccaggct gttttccggg cgtgttccgt cagcctttga cgccaattat
      121 gggttcgatt cccacattca ttcctgaaat gaatactggt tttaacttat taaataaaaa
      181 gtgagggatt gcgcagtcag cgatccccga taacgtcgcc gataaagatg ccgggtaaca
      241 ggcaaaaggg aattcccctg tcgcttcgtg tctgcttccg cgacggctgt taagcaagga
      301 atagataatg attaaaaaaa taagcgtaag aaaagatcag ctggcattac tctccagaaa
      361 cggcgattac tacaaagtgc tacatgcagg cgagcatctt ttaccctggt taaatacgcc
      421 agaagtcttg ctgattacgc tcgatggcag cgaagttccc gacgtgctgg cggattattt
      481 gcgccgcttt cagcctgatt gggtggagaa atattgcctg gtggcagact tgtcggagat
      541 tgaagccggg gcgttgtata tggacggtat tttgcaggag atcctgccgc catcaacgcg
      601 ccgtttgtac tggcgtgtag aggatgattt gacactggtg cggatgaata cgcagcaagt
      661 ccaggtacaa acggaggtga tgaatgcggt attgcaacca cggcgcaaag gggcagttaa
      721 aggccgcgat gcaatactaa cggtgcaggt tccggcctgg catgtaggcg tgctgaaaat
      781 cgatggtgaa acgcaggcgc tgttaccgcc ggggctgacc gcatactgga aaattaacca
      841 tctggttgac gctgaagtgg ttgacacccg cttgcaggta ctggaagtca gcgggcaaga
      901 aatcttaacg aaagataagg tcaatctgcg gattaacctc gccgctaact ggcgctacag
      961 cgacgtgtta ttggcattta gccagttaac gaagccgatt gatcatttgt atcgggaatt
     1021 acaatttgcc ctgcgtgaag cggtaggtac gcgtacgttg gatgaattgc tggaagataa
     1081 acaggtcatt gatgacgttg tcagcgaaca ggtgaaatcg cgcatgttgc catttggtat
     1141 ggagattgcc tcgttagggg tgaaagatat cgtcctgcct ggcgatatga aaaatatcct
     1201 tgcgcagctg gttgaggcgg agaaatccgc gcaggccaat gtgattcgcc gacgtgaaga
     1261 aacggcagcg acgcgttcgt tactcaatac cgctaaagtc atggaaaaca acccggtagc
     1321 gttacgctta aaagaactgg aaacgctgga gagagtggct gaacggatcg ataacatctc
     1381 tgtatttggc ggtctggatc aggttctgca tgggttggtt aatatcaaag ggtgagtaac
     1441 atgccattaa atggagtcag tgctgcgatg cgagaaaggg tatcgcagca gttaaaagaa
     1501 attgaacggc ggtatgatgt gaaagtactg tacgcctgtg aatcagggag ccgcggctgg
     1561 ggatttgcct cgcccgacag cgactacgat gtacggtttt tgtatgttca cccgctggaa
     1621 tggtatctgc gagtagagtc accgcgcgat gttattgagc tgcccatcga cgacgagtta
     1681 gatgtttccg gttgggagtg gcgtaaggcg ctgggattat tgaaaggcgc gaacccgacg
     1741 ctgatagaat ggctggattc tccggtggtt tatcaacaag atgaagaaac gataacggcg
     1801 tttaaggcaa tggtcccaat gtggttttct ccgctgcgtg cgcgctggca ttactattcg
     1861 atggcgcaga aaaacttccc cggatatttg cagggcgacg aagttcgact gaaaaaatac
     1921 ttttacgtgc tgcgtccgct gctggccgtg cgctgggtcg aagcgggtaa aggcgtaccg
     1981 ccaatgcgct ttgctgagtt actggcggga agcgaacttg atgcgccgtt acgtgcagaa
     2041 atagacgaat tactggaacg taaacagcgc gcaggagaag cggaatatgg tccgcgccgt
     2101 ccattgttac atgcgtttat tcgtgcggaa ctggcgcgtg gggaaattcc accgctgtta
     2161 ccggatagca gagaagggga cgttaaggaa ctggatagct taatgtacca gacggtaatg
     2221 cgccgggcat aaagcccggc gttatttcac gccatcagct cttattatta ttgtgtattc
     2281 ccgctgattt cattctgtat aagcgttgca atatcgtcat tcgttgccgc ttgtgaacta
     2341 aatatcatct tctcacgcga atacaccgca aacttatctg acaggttcga agcaaacacc
     2401 ttgcgggcat tttctgccag caaaccggtg acgatatcat ggtggttttt atccactttt
     2461 cgggcaatat ttcccgcctc tttgtcctta ctataccgtt cattggtgct ttcccagctt
     2521 aatgccgctg aggttgtttt tatcatgact tctgtttggt agcctgcatc tttgagtttt
     2581 ttaatgtttg taacggcgtc gcggcattac gaaatgttcc ttcgacaaca atgttgagtt
     2641 tactgactat cgctctctca attgcacgtt cgaccgtttc gccggaaaat ttcgctgtat
     2701 gggtaacaaa atcatcccca taattttggt agatataatt aaaatcaggg tgtagaaatc
     2761 gaaaatcatc accgttaata atcattatat tatcttttgt ctctcttttg gctaattcaa
     2821 tcagagatga tttacctgct ccgggttgcc cacctaaaac aaaaccaatg ggatgctgtt
     2881 gcggtgtgac ttcatgattt gtcagacgct gccaaataga atcaaaagat gctttatttt
     2941 ctgaatgcgt agtattgttg aattcatgct gatattgagt tcttacctgc tgaaggagaa
     3001 tcttaccttg tgtaaaatca acaagttcag cgggggtaga ataaagataa tcgcgatagc
     3061 tgactaatcg cttgagagca tcgcctgaat cctgatgcga cgaaactgag caattatcaa
     3121 tgatatttga taaaaacgcg atgcactgac tggctccgtt taaaagtgca tctttttcca
     3181 ttaaagtagc cattaacact cctgttaaat gatatttttc ggtgattata atactgttct
     3241 acaggagtga ttttgctttt cagatattat ttaatctatg ttttcagtga ggatggcgta
     3301 taacaatcgg tgagcgttat acgccagtag ggaaatatta ctcaaacaaa ttaaaatgca
     3361 gcttctgcac cacctgctcg gcatcttcgc cgggcaccag gaagcacagg ttatggctgg
     3421 atgcgccgta acaaatcatg cgaatgttga acggttccag tacgccgaat acctctttgc
     3481 caacgccgca ggcttttgac aggtcattgc caatcaacgc gaccagcgcc agaccttctt
     3541 ctacttccac ccggcacagt gcggaaagct ccatcagcag agattgcgtc agcaacgtat
     3601 cgccagtgga ggttgaaccg gtggtatcaa gggttaatgc cacgctcact tctgacgtgg
     3661 tgattaagtc taccgaaata ttatgccgcg cgaggatgcc gaaaacttcc gcgaggaagc
     3721 cgcgagaatg cagcatattc aggctgtgca aagtgagcag agtctgattg cgacgaagcg
     3781 ccagcgcgcg gaacagcggc ggattttcag ttttattgca caccagcgta ccacctgcgc
     3841 gtgggtcttt gctggagccg acaaagaccg ggatatcgct gcgtactgcg ggtagcaacg
     3901 ttgccggatg cagtactttt gcaccaaaag ttgccatctc tgccgcttcg gcaaacgcga
     3961 tttcatcaat gcgttttgct gcggaaacta cgcgtggatc ggtggtgtag atgcccggga
     4021 cgtcggtcca gatatcaaca cgagatgcgt gtaaagcctc cgccagcaag gctgccgtat
     4081 aatcgctgcc tccacggcca agcgtcgttg tacgaccttt attttcgcta ccgataaatc
     4141 cctgggtgat cactaagcct tcattgagac gtgggagcag ctgcagcgcg gccagttccg
     4201 ccagcgcggc tatatctggc tctgcacgac caaatcggtc gttggtacgc atcactttac
     4261 gtacatcaaa ccactgtgcc tgaacatcgc gttcgcgcag gatctcaaca aacagcaggg
     4321 tcgacatcag ctcgccgtgg ctgaccagct catctgtcag cgccggagac gttgccagcg
     4381 ccgccgcttc tgccagaaca gtaatgttct ccagcagacg ttcaatctct tcacggataa
     4441 cgttcgggta acgcagacgt tccagaatgg caaactggat gttgcggata gcgtcgagtt
     4501 tttcgaatcg ctcgccaggt tccagtcctt cagctaaagc gaccagcaga ttagtgatac
     4561 cagcagaagc cgagaggaca actaaacgca cgttggcatc agaaagcaca atatcagcgc
     4621 tgcggttcat ggcgtcaaaa tcagctacgc tggtaccgcc aaatttggag acaacaattt
     4681 cagacataac tacctcgtgt caggggatcc attttcagcc ttggcacaag ggaagagcgg
     4741 aagacgggtg ggcgcagagc gatacttcgc tactattttc acccagaagt gctccaccac
     4801 ttgcgaaacg cccgactgcg aacgcttctg gtgacaaccc aggggattca gcccctgtag
     4861 ccgatgatga acgtggccag ccgttcaatc acctcggcga tgcaccccct caggtgttat
     4921 cacaggactg gctcctccaa caccgttact tgggcaacgc gcctcttctg gcctgcgcta
     4981 gcgcaggtag tacatttata aataaagggt gagcggggcg gttgtcaacg acggggtcat
     5041 gcggattttt catccactcc tggcggtcag tagttcagct aataaatgct tcactgcgct
     5101 aagggtttac actcaacatt acgctaacgg cactaaaacc atcacatttt tctgtgactg
     5161 gcgctacaat cttccaaagt cacaattctc aaaatcagaa gagtattgct aatgaaaaac
     5221 atcaatccaa cgcagaccgc tgcctggcag gcactacaga aacacttcga tgaaatgaaa
     5281 gacgttacga tcgccgatct ttttgctaaa gacggcgatc gtttttctaa gttctccgca
     5341 accttcggcg atcagatgct ggtggattac tccaaaaacc gcatcactga agagacgctg
     5401 gcgaaattac aagatctggc gaaagagtgc gatctggcgg gcgcgattaa gtcgatgttc
     5461 tctggcgaga agatcaaccg cactgaaaac cgcgctgtgc tgcacgtagc gctgcgtaac
     5521 cgtagcaata ccccgatttt ggttgatggc aaagacgtaa tgccggaagt caacgcggtg
     5581 ctggagaaga tgaaaacctt ctcggaagcg attatctccg gtgagtggaa gggttatacc
     5641 ggcaaagcaa tcactgacgt agtgaacatc gggatcggcg gttctgacct cggcccgtac
     5701 atggtgaccg aagcactgcg tccgtacaaa aatcacctga acatgcactt tgtttctaac
     5761 gtcgatggga ctcacatcgc tgaagtgctg aaaaaagtaa acccggaaac cacgctgttc
     5821 ctggtagcat ctaaaacctt caccactcag gaaaccatga ccaacgccca tagcgcgcgt
     5881 gactggttcc tgaaagcggc aggtgatgaa aaacacgttg cgaaacactt tgcggcgctc
     5941 tcaaccaatg ccaaagccgt tggcgagttt ggtattgata ctgccaacat gttcgagttc
     6001 tgggactggg tcggcggccg ttactctttg tggtcagcga ttggcctgtc gattgttctc
     6061 tccatcggct ttgataactt cgttgaactg ctttctggcg cacacgcgat ggacaagcat
     6121 ttctccacca cgcctgccga gaaaaacctg cctgtactgc tggcgctggt tggcatctgg
     6181 tacaacaatt tctttggtgc ggaaactgag gcgattctgc cgtatgacca gtatatgcac
     6241 cgtttcgcgg cgtacttcca gcagggcaat atggagtcca acggtaagta tgttgaccgt
     6301 aacggtaacg ttgtggatta ccagactggc ccgattatct ggggtgaacc aggcactaac
     6361 ggtcagcacg cgttctacca gctgatccac cagggaacca aaatggtacc gtgcgatttc
     6421 atcgctccgg ctatcaccca taacccgctc tctgatcatc accagaaact gctgtctaac
     6481 ttcttcgccc agaccgaagc gctggcgttt ggtaaatccc gcgaagtggt tgagcaggaa
     6541 tatcgcgatc agggtaaaga tccggcaacg cttgactacg tggtgccgtt caaagtgttc
     6601 gaaggtaacc gcccgaccaa ctccatcctg ctgcgtgaaa tcactccgtt cagcctgggt
     6661 gcgttgattg cgctgtatga gcacaaaatc tttactcagg gcgtgattct gaacatcttc
     6721 accttcgacc agtggggcgt ggagctgggt aaacagctgg cgaaccgtat tctgccagag
     6781 ctgaaagatg gcaaagaaat cagcagccac gatagttcga ccaatggtct gattaaccgc
     6841 tataaagcgt ggcgcggtta atcatcgtcg atatgtaggc cggataaggc gttcacgccg
     6901 catccggcaa ccgatgcctg atgcgacgcg gtcgcgtctt atcaggccta caggtcgatg
     6961 ccgatatgta catcgtattc ggcaattaat acatagcacg attgattaaa taaccttaat
     7021 aacaatgccg acgttatgtc ggcatttttt tatcagataa atccccttgt ctgtgattta
     7081 ac
//
LOCUS       DAZFWL020000198         7060 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFWL020000198 DAZFWL020000000
VERSION     DAZFWL020000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.198"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..6241
                     /gene="efa1"
                     /locus_tag="ACHKNP_RS22895"
                     /old_locus_tag="pgaptmp_004579"
     CDS             <1..6241
                     /gene="efa1"
                     /locus_tag="ACHKNP_RS22895"
                     /old_locus_tag="pgaptmp_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HGU4706606.1"
                     /translation="VTIHNIDKVIFKGQTLDRLYHEGYVFSDINTLSRYTLHGLGITG
                     VHTEENLLPAPSSSLINILKEHYNEDEISAKLPLAYDYILNKKESSSIPVEILNKLSE
                     LPPHELLTPVLGQSVNPLGMGYSSDNGKITEQVIVSGADGFDNPISGLIYTYLEDLYN
                     IHVRMREGTLNSQNLRQLLENSVSSCFLTEQSINKLLSEAEKRPYQSLTEIHQHLTGL
                     PTIADATLSLLSVGLPGTGKLLRREQDYGRPPVTAIQDSTFVLPYNFKGIGFNDNIIS
                     SAPVVSSLHFIAEHAKYTLLSWPEFYRHHAQRWFEMAKGYGSQNIDFHPQSLLVTQEG
                     RCMGLALLYLQTEDTAHYSILQENLMTVSALHQTSNRDKLPLSKDDNSLMTRTYSLIE
                     MLQYQGNKYITNESLLHKTAWNQERITLLFNEKGVKRALISTPNHTLVLQQLEDIYRL
                     TDPNFGHADFLSPIDALKFIEAMIQLTPTLQEYYGLLNKDINKHIQVHYAESDMVWNK
                     LLPENDAGLSTRIQHTTTDRLANLAEPVAVAGISLPVKTLYDIGATLDGRRITSPPTS
                     EQIPSLRLNGDVLNDYLSRTVLTPEQADNIRKILHTQGIRSGTRPIDPEMIRGTQDDL
                     VSSQTRLQRQATRVKQQLAGVLDTLQQHFQNIPRSSGRHLSVENIELADIGSGRFNLQ
                     IRDGETLHTTSVEVPEVVSRFQKLSTMLSALPASGIMDFDLGMSVVGVVQYARLLQQG
                     HEDSTLAKINLAMDIKQLSEATLGSMIQIAGNKFLNTEGIQGFRLESAVAEGMRSVAT
                     RTGGTMGKALSASARVLELPVLETVLGTWNLYNSVIQLQQATSYSETMAARVQIAFDS
                     ISLGLTAASVAFPPLIIATGPIAAIGMGASSIARNVARKEERHTQWLEYKKFLTDGSK
                     HIVVASPERGLLDFSGNKVFGKMVLDLRQSPPLLHGESSFNADRKIGHRPDLGDWQIR
                     EKVGYANSISPYSSLAHGYANSKWPQTIPKIPSGEYDTIILGYGHQYQANTEIEYLSN
                     WIVWREAVPDSTSRHKRPPLEVLNSQCTVIAGERKTTVLPLRVLSDLTPECTEQAISL
                     KDYKFILRGGSGGLAVQVGGAGYYDIDANPVAKENTLSFRGLPEEFPLAFDLSKQTQS
                     VMLKTTDGEVPVMTITQKGINTLVGTTAGENWLTGNDKDNTFHTSSGGGTVISGGGNN
                     RYIIPRDLKTPLTLTLSNSSVSHEILLPETTLTELKPVAFELSLIYWAGNNIKVQLED
                     ETQLNRFAGNFRGHTSDGITLEAVSQENGIQLGVSSCDVQRWQAVYPEENNRPDAILD
                     RLHDMGWSLAPEVRFRGGETSASYDPLNRQLVYRLQERYSEFRLTGSRHYTTAVTGTP
                     GSRYIITGPDTAQISPAQIILAGDNDHPETIDLLEASPVLVEGKKDKNSVILTIATIQ
                     YSLQLTISGIDESLPGTTRVAIQPQDTRLLGDVLRILPDNGNWVGIFRSGHTPTVNRL
                     ENLMALNQVMTFLPRVSGSAEQVLCLENLGGVRKKVEGELLSGKLKGAWKAEGEPTVP
                     VNISDLSIPPYSRLYLIFEGKNNVLLRSKVHAAPLKITSAGEMQLSERQWQQQEHIIV
                     KPDNEAPSLILSEFRRFTISSDKKFSLKLMCHQGMVRIDRRSLSVRLFYLREQPGIGS
                     LRLTFRDFFTEVMDTTDREILEKELRPILIGDTHRFINAAYKNHLNIQLGDGVLNLAD
                     IVAEYARIQKEETSKILYQYQGAMKKKTDGPSVVEDAIMTTTVTTDSGEPFPTFHPWY
                     TDDLSGRYKSVPMARKADTLYHLTPKGDLQIIYQVATKMVNQAMIVSLPNYRHEWEKY
                     NLSILSEIPQNNNTVVHSILRVNGPTMQVRTIDYRGTDENNPIVSFSDTTFINGEQML
                     SYDSHSSGRVYSREEYMMWELQQRVSEASSARTQDYWLMDAAVRNGEWKITPELLRHT
                     PGYIRSTVSKWSRGWLKTGTILQTPEDRNTDVYLTTIQNNVFSRQGGGYQVYYRIDGM
                     AGADIADNAPGETRCTLRPGTCFEVTSVDERHYEWNIIYVTLKTCGWSRNGQSKTPNG
                     DNLFN"
     gene            6413..6763
                     /locus_tag="ACHKNP_RS22900"
                     /old_locus_tag="pgaptmp_004580"
                     /pseudo
     CDS             6413..6763
                     /locus_tag="ACHKNP_RS22900"
                     /old_locus_tag="pgaptmp_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2100 a         1599 c         1600 g         1761 t
ORIGIN      
        1 tgtaaccatt cataatatag ataaagttat atttaaaggg caaacactgg atcgtcttta
       61 tcatgaagga tatgtatttt ctgatatcaa taccttgtct cgttatacac tacacggact
      121 aggaataact ggtgtacata ctgaagaaaa cctgctacca gctccttcat cgtccttaat
      181 taatatattg aaagaacatt ataatgaaga tgaaattagt gcgaaattac cactagcata
      241 tgattacatt ttaaataaaa aagaatcaag ctctattcct gttgaaattt tgaacaaact
      301 ttcagagtta ccaccacatg aactactcac acctgttctt ggccagagtg ttaatcctct
      361 gggcatgggc tactcatccg ataatggaaa aatcacagag caagtaatag tcagtggagc
      421 tgatggattt gataatccca tatctggact tatatatacc tatcttgaag atctatataa
      481 catccatgta aggatgcgag aaggtacact aaattcacag aatcttcgtc agcttctgga
      541 aaactctgtt tcttcatgct ttttgactga gcaaagtatt aataaattac ttagtgaggc
      601 agaaaaaagg ccttatcagt ctttaacaga aatacatcag catctcacag gattaccaac
      661 tattgccgat gcaacccttt cattactttc tgttggatta cctggtacgg gaaaactatt
      721 gcgcagggag caggactatg ggcgtccacc agttacagca attcaggatt ccacatttgt
      781 actcccttat aatttcaaag gtattggttt taacgataac attatatcct ctgcacctgt
      841 agtctcctca ttacatttta tcgctgaaca tgcgaaatat actttgttgt catggcctga
      901 gttttatcgt catcatgcac agcgatggtt cgaaatggct aaaggatatg gaagccagaa
      961 tattgatttt caccctcagt ctctattggt aacccaagaa ggacgctgta tgggattagc
     1021 cttactttat ttacagactg aagatactgc tcattatagc attctccagg aaaacctaat
     1081 gactgtgagt gcacttcatc agaccagtaa tcgcgataag ttgccactgt ccaaagatga
     1141 taattcctta atgacaagaa cttatagtct gattgaaatg ctacagtatc agggaaacaa
     1201 atatattacc aacgaatcgc tactacataa gaccgcatgg aaccaagaaa gaataacttt
     1261 attattcaat gaaaaaggag ttaagcgagc cctgataagc acgcctaatc atactctggt
     1321 tctgcaacaa ctggaggata tttaccggct cactgatcca aattttgggc atgcagattt
     1381 cctttcacct atagatgctc tgaagtttat tgaggctatg atacaattaa ctccaacact
     1441 tcaggaatat tatggcctat taaacaaaga cattaataaa catatacaag tacattatgc
     1501 cgaatcagat atggtctgga ataagcttct gccagaaaat gatgctggac tgagcaccag
     1561 aattcagcac accaccaccg accgtctggc gaatctggct gaaccagtcg ctgttgcagg
     1621 tatctccctg ccagtaaaaa cactttatga tatcggagcc acccttgacg gtcggcgcat
     1681 cacctctcct ccaacatcgg agcaaatccc ttctctgcgt ctcaacggtg atgttctgaa
     1741 tgattatctg tcccgcacag ttctgactcc agaacaggct gataacataa gaaaaatact
     1801 gcacactcag ggaatacgca gcggtacccg tcccatagat ccggagatga ttcgtgggac
     1861 gcaggatgac ctagtttcgt cacagactcg tctgcaaagg caagcaacac gggttaaaca
     1921 gcaactcgcc ggcgtacttg atactctgca acagcacttc cagaacattc cacgttcatc
     1981 cggtcgtcat ctttctgtag agaatattga gctggctgat atcggaagtg ggcgtttcaa
     2041 ccttcaaatt cgagatggag aaacattgca taccacttct gtggaagtac cggaagttgt
     2101 gtcccgtttt cagaaacttt ctaccatgct ttcagccctg cctgccagtg gaatcatgga
     2161 tttcgacctc ggcatgagtg tggtcggagt cgtccagtat gcccgcctgc tacagcaggg
     2221 gcacgaagac agtaccctag ccaagataaa tctagctatg gatatcaagc aactttccga
     2281 agcaactctc ggcagcatga ttcagattgc cgggaataag tttctcaata cagaaggaat
     2341 ccaggggttc agactggaaa gcgccgtcgc tgaaggtatg cgctcagtag caacccgtac
     2401 cggaggcaca atggggaaag ccctttctgc cagtgcccgt gttcttgaac tgcctgtact
     2461 ggaaacagtt ctggggacat ggaacctgta caacagcgtc attcagctcc agcaagctac
     2521 atcttattct gagacaatgg ctgcccgggt acagattgcg tttgattcca tttctctggg
     2581 attaactgcc gcttcggtag ctttcccgcc actaattatt gccactggcc ccattgcggc
     2641 tattggtatg ggagcttcca gtattgcacg taatgtggca cggaaagaag aacggcatac
     2701 acaatggctg gaatataaaa aattcctgac tgatggcagt aaacacattg ttgtggcctc
     2761 tccggaaaga ggtctgctgg atttctccgg aaacaaagtt tttggaaaaa tggtgctgga
     2821 tctgcgtcag tctcctcctc tcttgcatgg agaaagctct tttaacgctg accgcaaaat
     2881 cggtcatcgt ccggatctgg gggactggca aattcgtgag aaggtggggt atgccaacag
     2941 tatcagtccc tactcttccc tggcgcacgg ttacgccaac agtaaatggc cacaaacaat
     3001 accgaaaatt ccctcgggag aatatgacac cataattctg ggctacggtc accagtatca
     3061 ggccaatacg gaaatagaat atctgtctaa ctggattgta tggcgggaag ccgtaccaga
     3121 cagtacttcc cgccacaaac gtcctcctct ggaggttctt aatagtcagt gtactgtgat
     3181 agctggagag cgtaaaacca cagtacttcc cctgagagtg ctcagcgatc tgacaccgga
     3241 atgcacagaa caggctatat cgttaaaaga ttataaattc atactgagag ggggaagcgg
     3301 tgggctggct gttcaggtcg gtggcgcggg atattatgat attgatgcaa atcctgtggc
     3361 aaaagaaaat acgctctctt ttcgcgggct accggaagag tttccgctcg cctttgattt
     3421 atcaaaacaa acacagtcgg tcatgctgaa aacaacggac ggtgaggtgc cggtaatgac
     3481 catcacccag aagggaataa acaccctggt cggtacaacg gccggcgaaa actggctaac
     3541 gggtaacgat aaggacaata ccttccatac gagttctggt ggcggtactg tcatctctgg
     3601 tggagggaat aaccgctata ttatcccccg cgatttaaaa actccgctga cactgacgct
     3661 gtctaatagc tcggtctctc acgagatcct tctgccagaa acaactctga cagaattaaa
     3721 acctgtcgcc tttgagctga gtctgattta ctgggccggg aacaatataa aggttcagct
     3781 ggaggatgag acacaactga accgttttgc cggaaacttc agggggcata ccagtgatgg
     3841 cattactctg gaggcggttt cccaggaaaa tggtattcaa ctgggggttt catcatgtga
     3901 tgttcaacgc tggcaggctg tttatccgga agaaaacaac agaccagacg ccatactgga
     3961 caggctgcat gatatgggat ggagcctggc ccccgaggtc aggttccggg gaggagaaac
     4021 atctgccagc tatgaccccc tgaaccgtca gctcgtttac cggcttcagg agcgttactc
     4081 ggaattcagg ctgaccggca gtcgccatta caccacggct gtaaccggaa cccccggaag
     4141 ccggtacatt attacggggc cggatacagc acagatatca ccggcacaaa tcatactggc
     4201 cggtgataat gaccatccgg aaacgattga tttgcttgag gccagtcctg ttctggttga
     4261 agggaaaaaa gacaaaaaca gcgtgatatt aacgattgct acgattcagt attcccttca
     4321 actgacaata tccgggatcg acgaatcgct gcccgggaca acccgtgtgg caattcagcc
     4381 tcaggatacc cgtttactgg gtgacgtact ccggatctta ccagataatg gtaactgggt
     4441 ggggattttc cggagtggtc atacaccaac ggtaaaccgg ctggaaaatt tgatggcact
     4501 gaatcaggta atgacgttcc tgccccgggt atccggaagt gcagagcagg tattatgcct
     4561 cgaaaaccta ggtggcgtaa ggaaaaaagt ggagggggag ttactgtcag ggaagctgaa
     4621 aggtgcgtgg aaagccgaag gtgaacctac tgttccggta aatatctcag atctaagtat
     4681 cccaccctat tcacgtctgt atctgatttt tgaagggaaa aataatgtgt tgctacgcag
     4741 taaagtacat gcagctccgt tgaaaataac atccgcagga gagatgcagt tatctgaaag
     4801 gcagtggcaa cagcaggaac atattattgt caagcccgac aacgaagccc cctcattaat
     4861 actcagtgaa tttcgtcgtt tcactatttc atcggataaa aaattttctt taaaactgat
     4921 gtgccatcag gggatggtcc gcatcgaccg cagatcatta tcggtcagat tgttctatct
     4981 gcgtgaacag ccaggtatcg gcagtttacg tctgacgttc agagattttt tcacagaagt
     5041 gatggataca acggacaggg aaattctgga gaaagagctg agaccaattc tgataggaga
     5101 tacacaccgc tttatcaacg ctgcatacaa aaatcatctg aatatccagt taggagatgg
     5161 cgttctgaat ctggcagaca ttgttgcgga atacgcccgt attcaaaagg aagaaacatc
     5221 aaaaatattg tatcaatatc aaggtgccat gaaaaaaaaa acagatggac catctgtggt
     5281 agaagatgcc attatgacca ctactgtcac aacagattca ggtgaaccat tccctacctt
     5341 ccacccgtgg tatacagatg atttatcagg gcgttataag agcgtaccta tggcaagaaa
     5401 agcagatact ttgtatcacc tgacaccaaa aggtgatcta cagataatat atcaggtagc
     5461 tacaaaaatg gtgaatcagg cgatgattgt atccctgcca aactaccgac acgagtggga
     5521 aaaatataat ttaagcatct tatccgaaat ccctcagaac aataatactg ttgtacattc
     5581 aatcctcagg gttaatggcc ccacaatgca ggtgcgcaca attgactaca gaggaacgga
     5641 tgaaaacaat cccatagtat ctttttcaga tacaaccttc atcaatggtg aacagatgtt
     5701 gagttatgac tcgcattcat cagggcgagt ctattccaga gaagaatata tgatgtggga
     5761 attgcagcaa cgggtatcag aagcttccag tgcccggaca caggattact ggctgatgga
     5821 tgcagcggta agaaacggag aatggaagat cacaccagaa ttattacgtc acacaccggg
     5881 atatatccgg agtacggtat cgaaatggtc cagaggatgg ctgaaaaccg gcacaatact
     5941 ccagactcca gaagacagaa atacggatgt atacctgact accatacaga acaatgtatt
     6001 tagtcgtcag gggggcggct accaagtgta ttatcggatt gatggaatgg ctggtgcgga
     6061 tatagcggat aatgcaccag gggaaacccg ctgcaccctc aggcccggaa catgttttga
     6121 agtgacaagt gtggatgaaa ggcattatga gtggaatatc atttatgtca cgctgaaaac
     6181 ctgtggctgg agccgaaatg gccaaagcaa aacgccgaat ggtgacaacc tttttaacta
     6241 attaaaatct ctgttaaaga tgtataaccc ggcatctatg tgctcaccag catatagatg
     6301 caatgtgatt cccctgatgg tcgtatcaga gatggtatgc gagcctgtac atagatttgt
     6361 gtaattgcct gattttgata tgttcaatcc aacatcaaaa gcaggttaat ttatggacga
     6421 aaaacagttg caggctctgg ctaacgaaca ggccaaaaat ctcaaaaccc ctgaagatct
     6481 cagccagttc gatcgcctgc tgaagaaaat cagcgttgag gcagctctca acgctgaaat
     6541 gtcccaccat ctgggctacg ataaaaacca gcctaaaccg ggtgccaact cccgcaatgg
     6601 ctattccaca aagaccgtta tcaccggtga tggccccctg gaactacgca ctccgcgcga
     6661 tcgtgatggc tcttttgaac cgtaacttgt gaagaaaaac cagacccgga tcaccgggat
     6721 ggataaccag atcctgtcgt tgtacgccaa aggcctgaca accgtaagcg tacagcctga
     6781 accgtctggt cagaatctga cgaattagag taagctggac atccccagat atattatggc
     6841 tgccagcgtc atatcggcat aaatagagat atcccttaac gctaaccaga aaaaaatacc
     6901 tataagagcc tgtacataga gttgtgtcgt tgcctgatgt tgatatgtca aatccaacat
     6961 caaatagcgg taaatttatg gataaaaaat tacaggctct ggttaacgaa acacccccct
     7021 cttgctctat tcccctcttt catcatttat tacaatattt
//
LOCUS       DAZFWL020000199         7058 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFWL020000199 DAZFWL020000000
VERSION     DAZFWL020000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.199"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..818
                     /gene="gadX"
                     /locus_tag="ACHKNP_RS22905"
                     /old_locus_tag="pgaptmp_004581"
     CDS             <1..818
                     /gene="gadX"
                     /locus_tag="ACHKNP_RS22905"
                     /old_locus_tag="pgaptmp_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HGU4706607.1"
                     /translation="LHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKC
                     VENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKN
                     LRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLAR
                     IASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSV
                     SYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1186..1914
                     /gene="gadW"
                     /locus_tag="ACHKNP_RS22910"
                     /old_locus_tag="pgaptmp_004582"
     CDS             1186..1914
                     /gene="gadW"
                     /locus_tag="ACHKNP_RS22910"
                     /old_locus_tag="pgaptmp_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HGU4706608.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2277..5390)
                     /gene="mdtF"
                     /locus_tag="ACHKNP_RS22915"
                     /old_locus_tag="pgaptmp_004583"
     CDS             complement(2277..5390)
                     /gene="mdtF"
                     /locus_tag="ACHKNP_RS22915"
                     /old_locus_tag="pgaptmp_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HGU4706609.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5415..6524)
                     /gene="mdtE"
                     /locus_tag="ACHKNP_RS22920"
                     /old_locus_tag="pgaptmp_004584"
     CDS             complement(5415..6524)
                     /gene="mdtE"
                     /locus_tag="ACHKNP_RS22920"
                     /old_locus_tag="pgaptmp_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HGU4706610.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6632..6910
                     /locus_tag="ACHKNP_RS22925"
                     /old_locus_tag="pgaptmp_004585"
     CDS             6632..6910
                     /locus_tag="ACHKNP_RS22925"
                     /old_locus_tag="pgaptmp_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706611.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6911..>7058)
                     /gene="gadE"
                     /locus_tag="ACHKNP_RS22930"
                     /old_locus_tag="pgaptmp_004586"
     CDS             complement(6911..>7058)
                     /gene="gadE"
                     /locus_tag="ACHKNP_RS22930"
                     /old_locus_tag="pgaptmp_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HGU4706612.1"
                     /translation="LKNKNESNITSTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGI
                     TSYF"
BASE COUNT         1785 a         1694 c         1741 g         1838 t
ORIGIN      
        1 cattacatgg gaattgtcta attgcgtacg caagacataa atatattctc accatggtta
       61 atggtgaata tcgctatttt aatggcggtg acctggtttt tgcggatgca agccaaattc
      121 gagtagataa gtgtgttgaa aattttgtat tagtatcaag ggatacgctt tcattatttc
      181 tgccgatgct caaggaggag gcattaaatc ttcatgcaca taaaaaaatt tcttcattac
      241 tcgttcatca ctgtagcaga gatattcctg tttttcagga agttgcgcaa ctatcgcaga
      301 ataagaatct tcgctatgca gaaatgctac gtaaaagagc attaatcttt gcgttgttat
      361 ctgtttttct tgaggatgag cactttatac cgctgctttt gaacgttttg caaccgaaca
      421 tgcgaacacg agtttgtacg gttatcaata ataatatcgc ccatgagtgg acactagccc
      481 gaatcgccag cgagctgttg atgagtccaa gcctgttaaa gaaaaaattg cgcgaagaag
      541 agacatcata ttcacagttg cttactgagt gtagaatgca acgtgctttg caacttattg
      601 ttatacatgg cttttcaatt aagcgagtcg cagtgtcctg tggatatcac agcgtgtcgt
      661 atttcattta cgtctttcga aattattatg ggatgacgcc cacagagtat caggagcgat
      721 cggcgcaggg attgccgaac cgtgactcgg cggcaagtat tgttgcgcaa ggaaattttt
      781 acggcactga ccgttctgcg gaaggaataa gattatagaa ttttactcag acataaaaaa
      841 aacccggcat aggggaccgg gaagaggata gtctgccgtc tccagactaa taaaccgtta
      901 taacactccc tgttggcacg ggaaactttg tgctctcagt aagttaaata taacttttac
      961 tggaaataag atcagccatt tttttataaa cataagctat acgctgtgcg aaaatatatt
     1021 cgtgctgcat ttacttatta tcaattaact gttatgcaaa actactttgt ggataaattt
     1081 tggtcctacc aaatctggca gtttttgcgc taagaaacag tctggcaaca tttcattagt
     1141 atactgaaat tgaaataatc gcagtatgaa atataaggga taatcatgac tcatgtctgc
     1201 tcggtgatcc tcattcgtcg ttcattcgat atttatcatg aacagcataa aatatcgctg
     1261 cataacgaga gtatcgtgct gctggagaaa aatttggcag acgattttgc gttttgttca
     1321 ccggatacgc gacgactgga tatcgatgag ctgacagttt gccattactt acaaaatatt
     1381 cgtcagctac cacgcaattt agggttacat agcaaagacc gtctattaat taaccagtca
     1441 ccccccatgc cgctggtgac ggcgattttt gatagcttca atgaatccgg ggtaaattca
     1501 ccgatactga gcaatatgct ctacctttcc tgtttatcga tgttttctca taagaaagaa
     1561 ctgatcccct tacttttcaa tagcatcagc actgtttcag gaaaagttga acgccttatt
     1621 agctttgata tcgccaaacg ttggtatctg cgcgatatcg cggaaagaat gtataccagc
     1681 gagagtctca tcaaaaaaaa gttgcaggat gaaaatacct gtttcagtaa aatattactc
     1741 gcttccagga tgtcgatggc cagacgatta ctcgagttac gtcaaattcc tctgcatact
     1801 attgcagaaa aatgtggcta tagcagtacg tcgtacttta taaacacatt tcgacaatat
     1861 tatggtgtaa cgccacatca gtttgcgcaa cattcgccag gtaccttttc ctgacatatt
     1921 ttgcatttga atattggtca ggatctcaca cctgcttcat gtgaaactct tccctgatga
     1981 tttctgccgg gctaccggct agttctcttt cgcagtttgc caggcggtat aggtattttc
     2041 aggaggaaaa gactcggcat gtttgggatt attaagctga caattcatac gattacggga
     2101 atgtgggtca gtattgtgct gttcaaattg atgacgaatg gctggagtgg tttctatttt
     2161 cagtgctgtg tactaagcct ggtattcctg acggttagct ggttgttgag tggtgaatgg
     2221 ttagcaggaa agagtaaggc tgaaccttca cgttcaacct tactctcatt tacacgttac
     2281 gcttttttaa agcgggcaaa gagatgttcc actacaacga aaaagaccgg aacgaagtaa
     2341 attgccagca ctgttgcggc aaacatcccg cccatcacgc cggtacctac cgcgttttgc
     2401 gcgccagaac cggcaccatg actgataacc agcggcagca cgccgagaat aaaggccaga
     2461 gaggtcatca ggattgggcg taaacgcatc cgcgccgctt cgatgattgc ctctatcggc
     2521 gttttccctt ctttctgcat catctcaacg gcaaattcga caatcaggat ggcgttcttg
     2581 gcggaaagcc cgatggtggt cagcaaacca acctggaagt agacgtcatt acttaagccg
     2641 cgcagatcgg tggccagtaa tgcgccaacg acgcctaacg gaacaaccaa catcaccgag
     2701 aacggaattg accagctctc atagagtgcg gcgagggcga ggaacaccac gaccagtgaa
     2761 atggcataca gtgcaggagc ctggtttgag gataacgctt cctgatacga cagtccggtc
     2821 catgagtagc cgacgcctgc cggaagttta gcgaccaggt ctgccataaa tttcatagcg
     2881 tcaccggtac ttttcccggc cgccgcttca cctaaaatct ccattgacgg gatgccgttg
     2941 tagcgttcca atcgcggtga accataggtc cattcggtag acgagtaggc agaaagcggt
     3001 gccatcgtgc cagaggcgtt gcgtacatac cattggttga tgttatccgg caacatacgg
     3061 aacggcgtgc ctgcctggac atacactttt ttcacccgcc cctggttgag gaagtcgttc
     3121 acgtagctgc tgccgaaggc ggtggaaatt gtctggttga tatcagacag cgccacaccc
     3181 attgcctcag ctttcgcggc gttgacgttc actttgaaca tcggcgtatc ttccaggccg
     3241 ttcgggcgta ccccggtgac ctggtcaggc gattgcgctg ccagtgataa cagctcgttt
     3301 cgtgcctggg ttagtttttc gtgccccagg ttaccgttgt ccagcagttc catatcaaaa
     3361 cctgacgcgg tacccagttc agccaccgcg ggtaagttga acgggaagac gacggcttta
     3421 ttgatactgc ttaacgcaat cattgcccgc tgaatgatcg cggtaaccga gttttcctca
     3481 ccgacacgtt cagaccacgg cttgagactg ataaacgcca ggccgttgtt ttgcccctgg
     3541 ccgctgaagc caaagccgcc aacggtaaac accgactgaa cattatcttt ctcttttgta
     3601 agataataat ccgtcacctg ttgcagcact ttcgtggtgt taaccatggt ggcaccggaa
     3661 ggtaactgcg cggtggtcat aaataccccc tgatcctctt ctggtaagaa agaggtcggt
     3721 gtacgcagga acagcaccgc catcccggcg caaatcagca ggtagaccac catgtagcga
     3781 ccggtacaac gcaacagcga gcgggtgcta tcggtatagt gctgagttga tttttcaaac
     3841 agcgtgttga agcgtgcgaa cagggcgtta ggtttgtgac cgccttccgg cgcggctttc
     3901 agaatggtgg cgcacagggc aggggtcagg ctcattgcca caaatactga aagcagcatg
     3961 gaggagatca gcgtgatgga gaactggcgg tagatctccc cggttgcgcc gctcataaag
     4021 gccatcggca taaacactgc ggaaagaaca acagcaatac cgaccagcgc acgttggatc
     4081 tgccccatcg atttatgcgt cgcttccttc ggtggtagct tatcttccgc aatgacacgc
     4141 tcgacgttct ccaccaccac gatggcgtca tccaccagta accctatcgc cagcaccatc
     4201 ccgaacatag ttaacgtgtt gatggtgaaa ccgaccgccg acaagatcgc aaacgtcccg
     4261 agaataacca ccggtacggc aatcgtcggg atgattgtgg cacggaaatt ctgcaaaaac
     4321 agatacatga ccaggaagac gaggatgata gcctcaacca gtgttttaaa aacttcctga
     4381 atagaaattt cgataaacgg cgtggtgtcg taaggataaa ccgtcttcag acttgcgggg
     4441 aaataggctg ataagcggtt cagctcctct ttgactgccc gcgaggtatc cagggcgttt
     4501 gctccggtag ccagtttgat ggcgatcccg gcagcaggtt tgccgttata gcgcgccacg
     4561 gtggaataat cttccgctcc aagttcgacg cgggcgacat cacgcagcag cacttgcgaa
     4621 ccatcttgct gaactttcaa caggattttg ccaaattctt ccggcgtttg cagacgcgtc
     4681 tgcacaatga tcgaggcgtt tagttgctgg tctgccgcct gtggcatgcc acccagttga
     4741 ccaccggaaa tctggttgtt ttgcacctta atctgggaaa taacatcgga aggtaccaga
     4801 ttgtatttat tgagtttttg cgggtccagc cagatacgca tggcgtactc ggaaccaaag
     4861 agctgtacgc taccaacgcc cgcggtacgg cttagcgggt ctttgatatt agacgctaca
     4921 tagtccgcga tatcgtactg gttgaggctg ccgttatcag aaataaacgc cgctaccatc
     4981 agaatattac tgctcgactt atcaacgcta atcccctgct gctgcaccgc ttcaggtaat
     5041 gaaggcatag cgagttgcag tttattttgc acctgaacct gtgcgatatc aggagatgtc
     5101 ccagtctcga aggtcagagt gatagaggca ttacccgccg catcactggt tgaagacatg
     5161 tacatcaggc catcaagccc attcatattt tgctcaatca cctgagtgac cgagtcttct
     5221 accgtttgcg catcggcacc tggataggta gcgctgacgg taatggtcgg tggcgcaatc
     5281 tgcggatact gcgcaaccgg taagttcatg atcgccagac cacctgcaag catcataata
     5341 atggcaagta cccaggcaaa aaccgggcga tcaataaaat agttagccat gaaagtcccc
     5401 ttaagcctgc aacgttattg tttcgattcg gtgctggcgt tttcctggct ggaggaaatt
     5461 gctcgtgctt tgatacccgg acgaatgcgt tgcaaaccgg aaacgatcac ccgatcgcca
     5521 gcctgcaagc cagaggtgac aacccactgg tcgccgatgg ctttgctggc ttcaatttcg
     5581 cgtagctgca caacatcgtc tttatccaga atgagcgccg ttgctttacc ctgggcgttg
     5641 tgggtgacgc cttcctgcgg caccagtaat acattctggc ggctaccttc atccactaat
     5701 gccgtgacgt acatgccagg cagcaagtca ccatttgggt tggggaaaat cgcccgtaac
     5761 gtcacggagc ccgtggtttc atccaccgtc gggtcggaga atttcagcgt gccggtctgg
     5821 ctgtagcgtt taccattttc cagattgagc tgtactggcg tactgccctg aacctgtttg
     5881 atttgcccac tggcgacctc ttctttcatg cgtaagaaat cttgcaccga ctgcgtgaga
     5941 tcgacataaa tcgggtccag acgttgtacg gtaaccagcg aatctgcctg attagcggta
     6001 acgagtgcgc cgacggtcac cgacgatttc ccgctgacgc ccgtaatcgg cgaggtgaca
     6061 ttcgcgtatt gcagattgat cgtcgcctgt tcaacagccg ctttggcgac ggtgacattg
     6121 gcttctgctt cattcaactg ggtgcgcgcg gtatcgtaat cctgacggct aacgtagttg
     6181 gtcttcagca acgatgcctg gcggttaaag gtgatgcggg cattgctggc ggtagagagc
     6241 gctttcgcca gcgagccttt ggcggagttt agctcggcct gtaaaggtgc aggatcaatc
     6301 tgatacagcg aatcgccctg gttcacttta tcgccttcga taaagttgcg tttaatgata
     6361 ataccgccca cctggggacg tatctcggca acttcataag gaacggttct accgggcaat
     6421 tcgctcaaca cattgaccga accgggggag agtgtgacga caccgacctc aggcgtcatg
     6481 gcggcggcgt tttccgccga tttgtcatcg caggcggtga gcatcgcgcc gcagaataac
     6541 aacggtatta acagctttct tcttctgttc attttagtcc ctgaaaattc ttgagaaata
     6601 acggttcttt aaattgcgcg cgaggagaga tatgagggat ttacagacca gcggcatagt
     6661 ggggttgtca gccagtaagg tgggatacag gcacagtgat cgacatggtg aggtcaacga
     6721 caaaaaaaat gttgccgttc tgccaacagt tcctgccagc attcgggcaa cgaagggttc
     6781 tactggtgga tacacatacc aggggaataa agtcgtttta gcctctcctt tagtaatcac
     6841 cgggggtaat gaaatcagca tctgcatccc tcgtcatgcc agccatcaat ttcagttgct
     6901 tatgtcctga ctaaaaataa gatgtgatac ccagggtgac gatgtcgctc atacgtctta
     6961 gtttcagctt gtacataatg ttgaatttct gaatctttaa tgtttgttgc gaaatattga
     7021 gcgtcgacgt gatattgctt tcatttttat ttttcaga
//
LOCUS       DAZFWL020000200         6866 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFWL020000200 DAZFWL020000000
VERSION     DAZFWL020000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.200"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            209..1366
                     /gene="acrE"
                     /locus_tag="ACHKNP_RS22935"
                     /old_locus_tag="pgaptmp_004587"
     CDS             209..1366
                     /gene="acrE"
                     /locus_tag="ACHKNP_RS22935"
                     /old_locus_tag="pgaptmp_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HGU4706613.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1378..4482
                     /gene="acrF"
                     /locus_tag="ACHKNP_RS22940"
                     /old_locus_tag="pgaptmp_004588"
     CDS             1378..4482
                     /gene="acrF"
                     /locus_tag="ACHKNP_RS22940"
                     /old_locus_tag="pgaptmp_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HGU4706614.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4735..4956
                     /locus_tag="ACHKNP_RS22945"
                     /old_locus_tag="pgaptmp_004589"
     CDS             4735..4956
                     /locus_tag="ACHKNP_RS22945"
                     /old_locus_tag="pgaptmp_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706615.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5387..6412
                     /locus_tag="ACHKNP_RS22950"
                     /old_locus_tag="pgaptmp_004590"
     CDS             5387..6412
                     /locus_tag="ACHKNP_RS22950"
                     /old_locus_tag="pgaptmp_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4706616.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6480..>6866
                     /locus_tag="ACHKNP_RS22955"
                     /old_locus_tag="pgaptmp_004591"
     CDS             6480..>6866
                     /locus_tag="ACHKNP_RS22955"
                     /old_locus_tag="pgaptmp_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU4706617.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIE"
BASE COUNT         1667 a         1639 c         1756 g         1804 t
ORIGIN      
        1 ttctgcctct ttcaacccgc gtcaaaataa aacagtagaa tattaatctt tttttgtgtt
       61 tatgtgcctt gagacgccag tattcataac tattccttac atcgacgaat gataatttgt
      121 aggatagcaa actgtatttt tctttctgcg agttaacgcg ttacctttct gggtaaataa
      181 cgcgcttttg gttttttgag gaatagtaat gacgaaacat gccaggtttt tcctcctgcc
      241 ctcctttatt ctgatctccg cggctttaat cgccggttgt aacgataagg gagaagagaa
      301 agctcacgtc ggtgaaccgc aggttaccgt tcatattgtg aaaacggccc cgttagaagt
      361 taagactgaa ttaccaggcc gcaccaatgc ttaccgtata gccgaagttc gcccacaggt
      421 tagcgggatc gtactgaatc gtaatttcac tgaaggcagc gatgtacaag caggccagtc
      481 cctgtaccag atcgatcccg cgacctatca ggcaaattat gacagcgcga aaggcgaact
      541 ggcgaaaagt gaagccgccg ccgccatcgc gcatttgacg gtaaaacgtt acgttccgct
      601 cgtgggtacg aaatacatca gccagcagga gtacgaccag gccattgctg atgctcgcca
      661 ggccgatgcc gccgtgattg ccgcaaaagc cacagtcgaa agcgctcgca tcaatctcgc
      721 ttataccaaa gtgactgcgc caattagcgg acgtatcggc aaatcgaccg tgaccgaagg
      781 cgctcttgtc actaatgggc aaacgactga actggcgact gtccagcagc tcgatcctat
      841 ctacgttgat gtgacccaat ccagcaacga ttttatgagg ctgaagcaat ccgtagagca
      901 aggaaatttg cataaggaaa acgccaccag caacgtagag ttggtcatgg aaaacggtca
      961 aacctatccc ctgaaaggta cgctgcaatt ctctgatgtg accgttgatg aaagcaccgg
     1021 ctccataacc ctacgtgctg tcttccctaa cccgcaacat acgcttttgc cgggtatgtt
     1081 tgtgcgtgca cggattgatg aaggcgtcca acctgacgcc attcttatcc cgcaacaagg
     1141 cgttagccgc acaccgcgtg gtgatgcaac cgtgctgatt gttaacgata aaagtcaggt
     1201 ggaagcgcgc cctgtcgttg ccagtcaggc gattggcgat aaatggttga ttagcgaagg
     1261 tctgaaatct ggcgatcaag tcattgtcag cggcctgcaa aaagcgcgtc cgggagagca
     1321 ggttaaagcc actaccgata cccccgcaga tactgcatcg aagtaaggta atctgacatg
     1381 gcaaactttt ttattcgacg accgatattt gcatgggtgc tggccattat tctgatgatg
     1441 gcgggcgcac tggcgatcct acaattgccc gtcgctcagt atccaaccat tgcaccgcct
     1501 gcggtttctg tttcggcaaa ctatccaggt gctgatgcgc agaccgtgca ggatacggtg
     1561 acgcaggtta tcgaacagaa tatgaatggt atcgataacc tgatgtatat gtcctccacc
     1621 agcgattccg ccggtagcgt gacaattacc cttaccttcc agtccgggac cgatcctgat
     1681 atcgcgcaag tgcaggtgca gaacaaactc cagctcgcca cgccgttgct gccgcaggag
     1741 gttcagcagc aggggatcag tgttgaaaag tccagtagca gctatttgat ggtggcgggc
     1801 tttgtctctg ataacccaga caccacacag gacgatatct cggactatgt ggcctctaac
     1861 gttaaagata cgcttagccg tctgaatggc gtcggtgacg tacagctttt cggcgcacag
     1921 tatgcgatgc gtatctggct ggacgccgat ctgctaaaca aatataaact gacaccggtt
     1981 gatgtgatta accagttgaa ggtacagaac gatcagatcg ctgccggaca gttgggcgga
     2041 acgccagcgt taccagggca acagttgaat gcctcgatta ttgctcaaac gcggttaaaa
     2101 aatccggaag aattcggcaa agtgaccctg cgcgtaaaca gtgacggctc ggtggtacgc
     2161 ctgaaagatg tcgcacgggt tgaacttggc ggtgaaaact ataacgttat cgctcgtatc
     2221 aacggaaaac cggcggcggg cctggggatt aagctggcaa ccggcgcgaa tgctctcgat
     2281 accgcgaaag ccattaaggc aaaactggcg gaattacagc cattcttccc gcagggaatg
     2341 aaggttctct acccttatga caccacgcca ttcgtccagc tttctattca cgaagtggta
     2401 aaaacgctgt tcgaagccat tatgctggtg ttcctggtga tgtatctgtt cttgcagaat
     2461 atgcgagcaa cgctgatccc caccattgcg gtacccgtgg tgttgttagg gacgtttgcc
     2521 atcctcgccg cttttggtta ctccatcaac acactaacga tgttcgggat ggtgcttgcc
     2581 atcgggctgc tcgtcgatga tgcgatagtg gtggtggaga acgtcgagcg cgtgatgatg
     2641 gaggataagc tcccgccaaa agaagcgacg gaaaaatcaa tgtcgcaaat ccagggcgca
     2701 ctggtgggta tcgcgatggt gctgtcagcg gtatttattc cgatggcatt cttcggcggt
     2761 tctactgggg caatttatcg ccagttctct atcaccatcg tttcggcaat ggcgctttct
     2821 gttctggtgg cattgattct tacccctgcg ttatgtgcaa cgctgcttaa acccgtctct
     2881 gctgagcatc acgaaaataa gggcggtttc ttcggctggt ttaatactac cttcgatcat
     2941 agcgttaacc actacaccaa cagcgtcggc aaaatcctcg gttccacagg acgatattta
     3001 ctgatctatg cgctgattgt tgcaggaatg gtggtgttgt ttttacgtct tccgtcttcc
     3061 ttcttacctg aagaggatca gggtgtcttt ctgaccatga ttcagttacc cgctggcgcg
     3121 acgcaagagc ggacgcaaaa agtgttggat caagtgacgg attactatct gaagaacgag
     3181 aaagcgaacg ttgaaagtgt ctttacggtt aacggcttta gcttcagcgg ccaggcacaa
     3241 aacgccggta tggccttcgt cagtctgaaa ccgtgggaag agcgtaatgg tgatgaaaac
     3301 agtgcggaag cggtaatcca tcgtgccaaa atggaatttg gcaagatccg cgacggtttt
     3361 gtcattccat tcaatatgcc agccattgtt gaactgggca cggcaacggg tttcgacttt
     3421 gagttaattg atcaggctgg gctgggtcac gatgccctaa cccaggcccg taaccagttg
     3481 cttggtatgg cggcgcaaca tcctgccagc ttagtcagcg tgcgtcctaa tggcctggaa
     3541 gacaccgcgc agtttaaact ggaagttgac caggaaaagg cgcaggcatt aggtgtttca
     3601 ctttctgaca tcaatcagac catttcaacg gcgttgggtg ggacttacgt taacgacttc
     3661 atcgaccgtg gtcgcgtgaa aaaggtgtat gttcaggcgg atgccaaatt ccgtatgctg
     3721 ccagaagatg tcgataaact ttatgtccgc agcgccaacg gcgaaatggt gccattctcg
     3781 gcctttacca cttcacattg ggtgtatggc tctccgcgac tggaacgcta caacggtctg
     3841 ccgtcaatgg agattcaggg ggaagccgcg ccaggaacca gttccggcga tgccatggcg
     3901 ttgatggaaa accttgcgtc aaaattacct gctggcattg gttatgactg gacgggtatg
     3961 tcgtatcagg aacgcttatc gggaaaccag gctcccgctc tggtagcaat ttcctttgtg
     4021 gttgttttcc tgtgccttgc tgcactctat gaaagctggt caattcctgt ctcggttatg
     4081 ttggtcgtgc cgttagggat tgtcggcgtg ctgctggcgg cgacactctt taatcaaaaa
     4141 aatgacgtct actttatggt gggcttgcta acgacaattg gcttgtcggc caaaaacgct
     4201 attttgattg ttgagttcgc taaagatctc atggagaaag agggtaaagg tgttgttgaa
     4261 gcgacactaa tggcagtacg tatgcgtctg cgtcctatcc tgatgacctc tctcgccttt
     4321 attctcggcg tattaccgct agctatcagt aacggtgccg gcagtggcgc gcagaacgca
     4381 gtgggtatcg gggtaatggg cggaatggtc tctgcaacgt tgctggcaat cttcttcgta
     4441 ccggtgttct ttgtggtgat ccgccgttgc tttaaaggat aaatcagaaa cataaaggca
     4501 ctttcgggtg cctttattat tttcagtgaa acccataaaa attaataaga tattcttcca
     4561 ctcactcttt aaaaactttc tatagttccc gctcccttca ctatttttac aattcacata
     4621 atttgtcatt attcattccg caatatttat aacctccggt gataaaatgg cattcagctc
     4681 gttaataaga gagttaactt attaagcgtt agcgtttatt actgaggtaa caccatgaaa
     4741 agattaattc ctgtggcatt gctcaccgca ttgctggcgg gctgtgctca cgattcgccc
     4801 tgcgttccgg tttatgacga tcagggtcgt ctggttcaca ccaatacctg tatgaaaggc
     4861 acgactcagg ataactggga aacggctggg gctattgccg gcggggcagc ggctgttgct
     4921 ggcctgacga tggggatcat cgctctgtcg aaataactga acagggcgcg gagtttcgcg
     4981 ctctgtttaa cgatacagta tctttacata agataaaata ttgctatatt ctgctggcta
     5041 attctctgct gatttagcat gttatatcgc gttatttcgc tgctttcgaa ataacgcatc
     5101 aatttaactt tcacacttcc caccagaaat atactcacgc taacaatcat tattctgctt
     5161 agtaaaacac gcgtaaatat tttgcgccaa aatgtggcgc atgtttcatt ttcgcaccat
     5221 tgcggggcgc tgtttttatt atttcctgtc tacactcagc ttattgcgac gcgaaatcgg
     5281 gcgcaaaagc tggcactact tttgcttata agaggatggc cacagacagg taaaagacgt
     5341 ttcccaaacg tcctataacg ataattttcg cctcacagga agcattatga aaaagatgat
     5401 gatagccaca ctggctgccg ccagcgtgct gcttgccgtt gcaaatcagg cgcatgctgg
     5461 agcgacgctt gatgccgttc agaaaaaagg ttttgtgcaa tgcgggatca gtgatggatt
     5521 acctgggttc tcttatgccg atgcagacgg taagttttca ggtattgatg ttgatgtttg
     5581 tcgtggtgtt gccgctgctg tatttggtga cgacacgaaa gtgaaatata ccccactcac
     5641 agcaaaagaa cgcttcacgg ctttacagtc aggggaagtg gatttgctct cccgtaatac
     5701 gacctggact tcatctcgcg acgccgggat gggaatggca tttactggcg tcacttatta
     5761 cgacggcatt ggcttcctga cgcacgataa agcggggcta aaaagcgcga aagaactgga
     5821 tggcgctacc gtctgtattc aggcgggtac tgataccgaa ctcaacgttg ccgactattt
     5881 caaggcaaac aatatgaagt acacaccggt gactttcgat cgctctgacg aatcagcgaa
     5941 ggcactggaa tctggtcgct gcgatacgct ggcctcggat cagtcgcaac tgtatgccct
     6001 gcgcatcaaa ttaagcaacc cagctgaatg gattgtctta ccggaggtta tctctaaaga
     6061 accgcttggg ccggtagttc gtcgtggcga tgatgaatgg ttctcgattg tacgctggac
     6121 gcttttcgcc atgctgaatg ctgaagagat gggcatcaat tcccagaacg tcgatgaaaa
     6181 agcggctaat ccagcaacgc ctgatatggc acatctgctg ggtaaagaag gcgattacgg
     6241 caaggatctg aagttggata ataaatgggc ctataacatc atcaaacagg tgggtaacta
     6301 ctcggaaatt ttcgagcgta atgtaggttc agaaagcccg ctgaaaatta aacgtgggca
     6361 aaataatctc tggaataacg gcggtattca gtacgcgccg cccgtgcgtt aagtcggtgt
     6421 gaggtaacga gcgtcgctac ggcggcgctc tcttcagcgt tatgctttcc gaggtttcta
     6481 tgtctcatcg ccgctcaacc gttaaaggct cactctcctt tgccaaccct acggttcgtg
     6541 cctggttatt ccagatcctt gccgttgttg ctgttgtcgg cattgttggt tggttatttc
     6601 acaacactgt aacgaatctc agtaatcgtg gcattacttc aggttttgcc tttctggatc
     6661 gcggcgctgg cttcggtatt gtccagcatt tgatcgatta ccagcagggc gacacctacg
     6721 gacgcgtttt tattgtcggc ttactcaata cgctactggt ttctgcattg tgtattgtgt
     6781 tcgcttctgt tttgggcttc tttatcggtc tggcgagact ttcggataac tggctgctac
     6841 gaaagctttc cacaatttat attgag
//
LOCUS       DAZFWL020000201         6809 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFWL020000201 DAZFWL020000000
VERSION     DAZFWL020000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.201"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(215..700)
                     /gene="sixA"
                     /locus_tag="ACHKNP_RS22960"
                     /old_locus_tag="pgaptmp_004592"
     CDS             complement(215..700)
                     /gene="sixA"
                     /locus_tag="ACHKNP_RS22960"
                     /old_locus_tag="pgaptmp_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HGU4706618.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(903..3047)
                     /gene="fadJ"
                     /locus_tag="ACHKNP_RS22965"
                     /old_locus_tag="pgaptmp_004593"
     CDS             complement(903..3047)
                     /gene="fadJ"
                     /locus_tag="ACHKNP_RS22965"
                     /old_locus_tag="pgaptmp_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HGU4706619.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGVVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3047..4357)
                     /gene="fadI"
                     /locus_tag="ACHKNP_RS22970"
                     /old_locus_tag="pgaptmp_004594"
     CDS             complement(3047..4357)
                     /gene="fadI"
                     /locus_tag="ACHKNP_RS22970"
                     /old_locus_tag="pgaptmp_004594"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HGU4706620.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4537..4821)
                     /locus_tag="ACHKNP_RS22975"
                     /old_locus_tag="pgaptmp_004595"
     CDS             complement(4537..4821)
                     /locus_tag="ACHKNP_RS22975"
                     /old_locus_tag="pgaptmp_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HGU4706621.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5193..6533
                     /gene="fadL"
                     /locus_tag="ACHKNP_RS22980"
                     /old_locus_tag="pgaptmp_004596"
     CDS             5193..6533
                     /gene="fadL"
                     /locus_tag="ACHKNP_RS22980"
                     /old_locus_tag="pgaptmp_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HGU4706622.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1696 a         1829 c         1620 g         1664 t
ORIGIN      
        1 tgacaatgag tgtcaaacag acattattca tttcaacatg taactttggg caaaatcaag
       61 atacgcgcag aagaataatt tccgaatgaa agtagaaagt gttttattta tagatgggga
      121 gagtcttcga tagtagtgag gttgacagat atccgctgcc tgatgagcaa ttatgcccaa
      181 tgaatctacc tcattgggca taatttggaa ctcatcagat agcttttgcc atcttcagat
      241 tacacgggct catctgccag ttgaatgtgc cgtttccgct ttcatcaagc gttacgctgg
      301 caatggccga ggtggtaaac ataggcggcg tttcgcccgg acataactcg gcaaccagat
      361 acccgactaa cggtaagtga gagatcacca gtaccgaagc aacaccttca ttggtcaacg
      421 cctgcaaata ggcactgacc agaccaacat cgccgcaggg cgttaactcc ggcagaactt
      481 ctgcactgga aggcaggttc agacaatccc ctacttcttc cagtgtttgc tcggctcgca
      541 ggaacgggct caccagaacg cgttcgattt ccactttttg acctttcagc cagttcgcca
      601 tcaggcgaga ttcgtcacaa ccattagtgg tcagaggacg aacggaatca ctggcggcat
      661 cgagggctgc gtcgccgtga cgcatgataa aaacttgcat attgcaccgc ttttgttaac
      721 cagattcgcc cgttttcttt catctgaaac cagagaaaga aaacggtggc cggcattgtg
      781 ccttatccat tcaccgaatg aaacgctgtt ttttacctca atggcgtaag tatagtcaat
      841 atgcgtttat attttgacca acactccgcc attcagcgcg gattcatata gctttgacct
      901 tcttattgca ggtcagttgc agttgttttc caaaaacttt ccccacgcgc gcccatctcg
      961 accaaacgct cgcaaggggt aaaacgggaa ccatactgcg tggcaagtcg ttgcattatt
     1021 gcaaccactt cgcccgcgcc gagagaatcg atatagcgga acggtccacc gagaaatggc
     1081 ggaaaaccaa tgccaaatac cacaccaata tccccgtcac gcacgctacg gataacctgc
     1141 tcatcaacac aacgtactgc ttcattcagc atcaacatca cacaccgttc agcaacctgc
     1201 ggtgcggaga gtcgcccctg cccttgtgcg ccaatgagcg ggtaaatggc gggatcgacc
     1261 tgttttttgc ttttacgccc tttctgacca taaagataga aaccccgacc attttttctg
     1321 cctttgcgat cgtcgttcaa aattgaagaa acaacatttg caggcgcgct aaaacgttct
     1381 ccataagcgg cttccagtac aggaataatt ttagtcccgg tgtcgattcc tacctcatcc
     1441 aaaagttgga ttggacctac cggaaaacca aatttcacta gcgcggcatc aatgtgctct
     1501 acccgttcac cttcggtcag catgcggata gcttcattaa tgtaaggcgc taagatgcga
     1561 ttgacgtaaa acccggcttt gtcacgcacg acaatgggcg ttttgccctg ttttttcgcc
     1621 agttttaccg tagtggcgat ggtttgcgcc gatgtacccg catgaggaat aatctctacc
     1681 agcggcattt tttccaccgg actgaagaaa tgcaggccaa taacttgctc aggtcgcgtg
     1741 gcgtgagctg cgatatcacc aatcggtaaa gatgacgtat tcgaagcaaa aatggtatga
     1801 gcggcgcaat tttgctccac ttccgccacc atctgttgtt ttaattcgag attttcaaac
     1861 accgcttcaa taatcagatc gcgatgggca aagccgcgat agtccgtcgt tccggagatt
     1921 aatgccagct gtttgtcacg ttcgctggct ttgagatgac gacggcgaac tttgccctcc
     1981 agctgatccc aactgtactt cagcgcatga tttatgccct gcgggttgat atctttaatt
     2041 ctgaccggaa gccccgcttt acaagcagtg acataagcaa taccgccgcc catcaagcca
     2101 ccacctaaaa ttcccacgct gtttaatggc gcaggcggtg catcactacc gggatctttc
     2161 ttcacttccg tactggcaaa aaagatacta cgcaaagctt gcgattgtgg cgtcatcgcc
     2221 agttcgccaa acgcccgagc ttcggcgtca taaccgctgc tggtgccctg cgctaatccc
     2281 gtttcaacaa cctccaggat gcgttctgtc gccggataat tgccttgagt tttgtgttct
     2341 gttttcttgc cgaccatttt gaacagcagc gcacgaccta acggccccgc cagaatacgc
     2401 tcgcgtacag gtagagggcg ggaagatggg cgatccttct tcgccagctc aacagcgact
     2461 tccagcaaaa tggagtgcgg aacaacgtca tccaccagcc ccagctttaa tgcctgtttc
     2521 gcccgaagtt gttttccggt gaggatcatc tctaatgctg tgctgacgcc tatcagacgc
     2581 ggtaaacgct gggtgccgcc tgaaccgggt aacaatccaa gttgtacttc aggcaaaccg
     2641 agcaccgttt taggatcgtc agtacaaacg cgaccgtggc acgccagcgc caactccagc
     2701 ccaccaccca ggcaagcgcc atgaatagcc gcgataaccg gaatgggcaa agcatgaatc
     2761 tccgccatca actgttggcc ctgccgcgcc agtgcttccg cttcttgcgc cgttttgcag
     2821 ttgccgatca tgttgatgtc tgcgccagca atgaagttgt ccggtttagc ggagacaaac
     2881 accacgcctc gcaactcttt gttttcacgg agttgcttaa taatggcgcg cacctgcgag
     2941 gcaaactccg ccttcagggt attcattttc tcacccggta cgtcgatggt gataacggca
     3001 atgttgtcca gacgaacatt aagggtaaac gctgatgtca tttccattat tccgcctcca
     3061 gaaccattgc cgcgccaagc ccaccggcag cacaggcggt aactaaacca aatccaccgc
     3121 cgcgacggcg aagttcatgc aatgtctggg taatcatccg tgcgccggtc gccgcgaagg
     3181 gatgtccgta agcaatcgaa ccgccaagca cgttaaattt gctatcgtcc acttcgccag
     3241 tggcatgtgc acgccccagt acttcacgag caaaacgttc actacccagc aactgaatat
     3301 tcgccagtgt ctgagctgca aaggcttcgt gcatatcgat caatgtcaga tcgctcattg
     3361 tcaaaccggc acgttccagc gccagcggtg ttgaccaggc cggaccgagc aacatgtcct
     3421 gccagacatc aatcgcagta aatgcgtagc tgcgcagata ccccagcggc accagcccta
     3481 attctttcgc ccgggattca gtcatcagga tcaccgctgc cgcgccatcg gtcagcggcg
     3541 tactgtttgc cgccgttacc gttccgtgtt tgcgatcaaa cgccgggcgc agctttgcgt
     3601 aatcggcaag cgaggaatta ccgcgaatat tgttgtcttc gacaagcggt tgtttataag
     3661 gagggataaa ggcagtcatc acctcttctt tgagttttcc gtctgaccat gcctgagcgg
     3721 cacgctgatg cgaacggtgc gctaatgcat cttgctgttc tcgggtgatg ccgtaggttt
     3781 tcgccatttg ttcggcggtg tcgcccatcc gcaagccagt agaatattct gctaccgcag
     3841 gcggtacggg cattaagtcg cgcaaacgca ggcgagagaa gagtttcagt cgctggctca
     3901 tggttcgagc tttgttgaca tcaaccagca cgcgcgccag ttttttactg acgccaattg
     3961 gcaatacgga agaggaatct gccccaccgg caatccccgc tcggatagtt cccgccatca
     4021 ggctttctgc gacgtttgca accgcctgga aactggtagc gcaagcgcgg ctgacgctgt
     4081 aagcatcggt atgcacattc attccggtac cgagaacaat ttcacgcgca atgttggggg
     4141 cttcaggcat ttgtacgacc tgaccaaaga ccagctgttc aatcacttca gcggggatct
     4201 cgctgcgtgc cagcagttcg cctaccacca tcttccctaa atcaaccgcg ggaatgccat
     4261 gaaaagccgt cgcctggcgg gcaaaaggcg tacgtaaacc gctaacaatg gcgatacgat
     4321 cgccctggcg ggtaaccagc ggtaaaacct gacccataac actcccctgt aaaaaaataa
     4381 ataaagtggt ctgacctgat catagtctta accatttttt tacatttagc caagcggaga
     4441 aaaaggaaag tgggagctat gacacagtga agacaggaga aagaaaagaa aacgcccctg
     4501 ccagcaactg acaggggcgt acgtacccga gaggaattaa cgcagaccca gctggaagat
     4561 cagcatttca gcttcgcagg caaaagtgaa atcgatatcc aggcgcacac cgtcagactc
     4621 ttcagtgaaa gtcggggtga ttttacacgg ttcggattcc acgctacggg ctttttcagt
     4681 cagcgccgcc agcgtttgtt ctgcttctgc gcggcttgca aacacgcggc tgtaagacgc
     4741 ggtgcagtcg gagttgtcca taatggtgcc aacatccata cagcagcaaa ccggggtttc
     4801 atcagcacta catttactca tcgttgattt cctctgtatg tgcacccaag gtgccagata
     4861 aacgttgtgg atattttacg cttccggaaa gtgctgctcc agttgttaat tctgcaaaat
     4921 cggataagtg accgaaatca cacttaaaaa tgatctaaaa caaaattcac ccgaatccgc
     4981 gagtgcgcaa cctccaaatt ttgccagctg gatcgcgttt cttagatcat atttgaaaaa
     5041 agatggaaac atacttgcaa cattccagct ggtccgacct atactctcgc cactggtctg
     5101 atttctaaga tgtacctcag accctacact tcgcgctcct gttacagcac gtaacatagt
     5161 ttgtataaaa ataaatcatt gaggttatgg tcatgagcca gaaaaccctg tttacaaagt
     5221 ctgctctcgc agtcgcagtg gcacttatct ccacccaggc ctggtcggca ggctttcagt
     5281 taaacgaatt ttcttcctct ggcctgggcc gggcttattc aggggaaggc gcaattgccg
     5341 atgatgcagg taacgttagc cgtaaccccg cattgattac catgtttgac cgcccgacat
     5401 tttctgcggg tgcggtttat attgacccgg atgtgaatat cagcggaacg tctccgtctg
     5461 gtcgtagcct gaaagccgat aacatcgcgc ctacggcatg ggttccgaac atgcactttg
     5521 ttgcaccgat taacgaccaa tttggttggg gcgcttctat tacctctaac tatggtctgg
     5581 caacagagtt taatgatact tatgcaggcg gctctgtcgg gggtacaact gaccttgaaa
     5641 ccatgaacct gaacttaagc ggtgcgtatc gcttaaataa tgcatggagc tttggtcttg
     5701 gtttcaacgc cgtctacgct cgcgcgaaaa ttgaacgttt cgcaggcgat ctggggcagt
     5761 tggttgctgg tcagattatg caatctcctg ccgggcaaac tcctcaaggg caagcattgg
     5821 cagctaccgc caacggtatc gacagtaata ccaaaatcgc tcatctgaac ggtaaccagt
     5881 ggggctttgg ctggaacgcc ggaatcctgt atgaactgga taaaaataac cgctatgcac
     5941 tgacctaccg ttctgaagtg aaaattgact tcaaaggtaa ctacagcagc gatcttaatc
     6001 gtgcgtttaa taactacggt ttgccaattc ctaccgcgac aggtggcgca acgcaatcgg
     6061 gttatctgac gctgaacctg cctgaaatgt gggaagtgtc aggttataac cgtgttgatc
     6121 cacagtgggc gattcactat agcttggctt acaccagctg gagtcagttc cagcagctga
     6181 aagcgacctc aaccagtggc gacacgctgt tccagaaaca tgaaggcttt aaagatgctt
     6241 accgcatcgc gttgggtacc acttattact acgatgataa ctggaccttc cgtaccggta
     6301 tcgcctttga tgacagccca gttccggctc agaatcgttc tatctccatt ccggaccagg
     6361 accgtttctg gctgagtgca ggtacgactt acgcatttaa taaagatgct tcagtcgacg
     6421 ttggtgtttc ttatatgcac ggtcagagcg tgaaaattaa cgaaggccca taccagttcg
     6481 agtctgaagg taaagcctgg ctgttcggta ctaactttaa ctacgcgttc tgataacgcg
     6541 ttcgcctgga taaagtcacc tgcatagcag gtgactttaa ctcccccact tcaccgaaag
     6601 tagtgtcctc attgcttacc tcctgagttt tgcaaacagc ctgatggcag cttgcccttt
     6661 caaatcaata agcggtatcc acagacaaat ctgaagggaa aggcatattt tcaggcgttc
     6721 tgctcgtcct tctcaaagag tttacttttc tgaatttcca ggatactcac ccccctggct
     6781 attgtgcgct catacactca aattaaaga
//
LOCUS       DAZFWL020000202         6802 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFWL020000202 DAZFWL020000000
VERSION     DAZFWL020000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.202"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHKNP_RS22985"
                     /old_locus_tag="pgaptmp_004597"
     CDS             complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHKNP_RS22985"
                     /old_locus_tag="pgaptmp_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HGU4706623.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHKNP_RS22990"
                     /old_locus_tag="pgaptmp_004598"
     CDS             complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHKNP_RS22990"
                     /old_locus_tag="pgaptmp_004598"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU4706624.1"
                     /translation="MKRRDTILRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHKNP_RS22995"
                     /old_locus_tag="pgaptmp_004599"
     CDS             complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHKNP_RS22995"
                     /old_locus_tag="pgaptmp_004599"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HGU4706625.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHKNP_RS23000"
                     /old_locus_tag="pgaptmp_004600"
     CDS             complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHKNP_RS23000"
                     /old_locus_tag="pgaptmp_004600"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5114..5116),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU4706626.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHKNP_RS23005"
                     /old_locus_tag="pgaptmp_004601"
     CDS             5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHKNP_RS23005"
                     /old_locus_tag="pgaptmp_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HGU4706627.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1490 a         1836 c         1836 g         1640 t
ORIGIN      
        1 atacgcttgt catcagtgac atacccataa ccttatcgat gtgttggagg gaagaacgag
       61 gcaaacagac aattgcggca tgatcttgaa tgggtaacgc ggaatacgag agatggtgtc
      121 gtcacaccct atgagctgta atccgggcga ccagcgccac ccggaaaacg tcagagatta
      181 ctccccttca cccggaaaca ggaacgggtt gatggaactg cgggcatagc cttcttgctc
      241 cattcgcgcg tccagtacca gagaggcgag gtcatctgcc acggcttcaa ctttcggttc
      301 tttttcctga taaagaatct tcaggtaagt gccgcagtca tcgcagcttt cggctttaat
      361 cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg ccgctctgct cgcagttgct
      421 gcattttacg cgcactacgt gccattcggt ttcacacagg ttgcagtgca ggtaacgcag
      481 accctgagtg gtgccaattt gcaccatgct ggacaccggc atagaaccac aaaccgggca
      541 atattgacgt tgctcgccgt attcagcgcg ggctttgccg gggatcagat tggccatctg
      601 cgcccagtag agcgacagtg cagcccagat aaacggcgct ttatcgctgc tgacggatga
      661 gaaatcagag gcaaacagtg cgctggccat atcttccagc tcctgagtcg atgccttctc
      721 cagattctca atcactgcca gcgccgggcc gctcatttca ggtttcagct cagcaatcag
      781 cgccatcagc agcttttgcc agtgcttatc acgcggcaga acgtgaatat ccagcggggg
      841 cttgccctgt gcgctggctt ctttaatgcg cgcggtcaga tccatctcca gcggatggtc
      901 gtacagcacc acttcctggg cgtgggcgat aagcgcagca aagcgcaggt aatcacccag
      961 cggattattt tctgccagct cgcgcagacg ctcggcgcgg cggttgtata aattcttgag
     1021 ccgagggaac aataacggcg gaatcatatc cgccgtacgt ttctcgctcg aacccagctc
     1081 atcttgcggg attatgcgaa tactcattca gctttctttt ccgttgtctt gcggacctca
     1141 cggtaccagc gcgggtgatg tttcttcgcc caggcgctgg taacccatcc ttccaccatg
     1201 gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat gcaccatgat aaccacaatt
     1261 aacgccactg cggcaaatga atgcagcatt aacgcgaatc ggatcaccgg gattgagaaa
     1321 gcaggcgcaa aataaggacg ccagataatc acgccgctca ccagcagcag aaccaggaaa
     1381 ataatcgccg cccagaaaac gcatttctga ccgaagttat aacgcccggt gtcacctact
     1441 tcctcgttga cgacgatctt acgaatattc ttcgcccaaa agatatcatc ccgattgatt
     1501 aggttgtgat gccagtaacg gaaaaacatg atgatgaacg aggcaaacat aaccacgccg
     1561 acaaacgggt gcagaattcg cgccagctgc ggtgtgccca tgatttgcat caaccagttg
     1621 aaggacggga acaaaaagcc cagcccgctc accgccgcca ggatgaagca gaaggcggtg
     1681 atccagtggt tgatacgttc cggcgcggtg tagcgcagga tggtgtcacg tcgtttcatt
     1741 tgcgctcctc gtctttctct tcgtgcagat tattctcttc ctcatccgca cggttcggac
     1801 cgacacccac gtagtggaag atactggccg caaaggtagc cgcaaagcca acagccgcga
     1861 gcggtttcca gatgcctttc cagaatttca cggtttcgct gatttccggg ttctccggca
     1921 agccatgata cagatttggc ttgtcagcat ggtgcagcac gtacattacg tgtgtaccac
     1981 cgacgcctgc cggatcgtac agacccgcat tgtcgtaacc gcgggttttc agctcagcaa
     2041 cgcgctcgct cgccagcgtt ttcatcgact ctttcgtacc gaagtgaatc gcgcccgttg
     2101 ggcaggtctt cacgcaggct ggttcttgcc caaccaccac gcggtcaacg cacagcgtac
     2161 atttgtagac gcggttgtct tccgggttga ggcgcggaat gtcgaacgga cagcccgcaa
     2221 tgcaataacc gcagccaatg cactgctcgg actggaagtc gacgataccg ttggcatact
     2281 gaataattgc accttccgcc gggcacgctt tcaggcagcc tggatcggaa cagtgcatac
     2341 agccgtcttt gcggatcagc cattccagtt tgtcgttctg ctccacttcc gagaagcgca
     2401 ttaccgtcca cgatttggcg cttaaatcat tggggttgtc gtacacccca atgttattgc
     2461 cgacggtatc gcgaatgtcg ttccactctg aacacgccac ctgacaggct ttacagccga
     2521 tacaggtggt gacgtcgatg agtttcgcca cttcttcctg gaagtcccgc gcctgaggcg
     2581 cgggggtcag accgttagtc gcggaacgac gaatgatatc ttgagattga taagccatat
     2641 gtcgtctccg ttacaccttt tccacattca caaggaagga cttaaactcc ggcgtctgcg
     2701 tgttcgcatc accaacgaat ggcgttaacg tattggcaat aaagcctttt ttagctacgc
     2761 cttcatagcc ccagtgaatc ggaataccga tggtatcgat atctttgccg ttcgctttca
     2821 gtgtacgaat acgtttggtc accaccgctt tggccttgat atagccacgg ttggaggaga
     2881 ctttcacggt atcgccctgg gcaatgccaa gtttattcgc cagcgactcc ccgatttcca
     2941 caaactgctc tggttgcaaa atcgcgttca acagcgcgtg tttggtccag tagtggaaat
     3001 gctcggtcag acgataggtg gttccgacat acgggaactt atcggcttta cccaatgctt
     3061 cggcgtcgtc tttaaagata cgtgcagccg ggttcgagat aacgtttgga tgcagcgggt
     3121 tagttcccag cggcgtttca aacggctcgt agtgttccgg gaacggacct tccgccatct
     3181 tatcgagggc aaacagacgc cccatgcctt cctgctgcat gataaacggc ccgacgccgc
     3241 tacccggagg cgctgcgctg tagtccggaa tatcccagcc ggtccactta gtgccgtccc
     3301 atttcagcaa ctgacgcttc ggatcccacg ggttaccctg cggatctgcg gaggcgcggt
     3361 tatacagaat gcggcggtta agcggccatg cccatgccca gcccagcgtg ttaccgaggc
     3421 cagacggatc ggcgttatca cgacgcgcca tctggttgcc ttccggcgtc cagctaccgg
     3481 cgaaaatcca gcagccacag gaggttgtac cgtcatcgcg caattgggcg aacgagctaa
     3541 gttgttggcc tttcttgacg ataaccgcac cggttgccgg atcggtaata tcggccagcg
     3601 ccttaccgtt gctctccatc gctacttctt ccgacttcgg ctcatgcgga atggcgtagt
     3661 tccaggtcat gttcagcacc tgatccgggt tcgcgccacc ctgttcggca tacatcttgc
     3721 gcaagcgcag gaagataccg gagaggatct caccgtcagt cagcgcaatc cccggggcgt
     3781 ccgcaccttt ccagtgccac tgcaaccagc gacctgagtt aacgattgaa ccgttctctt
     3841 ctgcgaagca ggtcgatggc agacggaaca cttcggtctg gatcttcgac gagtcaactt
     3901 cgttcagctc accgtggttc tgccagaagt tagaggtttc agtgttcagc gggtcgatgg
     3961 tgacgaggaa cttcagtttc gacagacaac cgatcacttt gtttttgttc gggaatgagg
     4021 caacagggtt aaagccctgg cagatatagc cattgacctt gccttctttc atcatctcga
     4081 agtattgcag gacgtcgtag cctttatccc acttcggcaa ccagtcaaag ccccagctat
     4141 tttccgccgt cgctttatca ccaaagaagg ccttcatcat agagacgaag aatttcgggt
     4201 agttgcccca gtagtttacc tggccttcca gcagcggttt tggcgtgttg gcggtaaggt
     4261 aggtttgcag atcggtctgc ttctcgcttg gcagcgtcat gtaacctggc agactctgcg
     4321 acagtagccc caggtccgtc agcccctgaa tattggagtg accgcgcagg gcgttaacgc
     4381 cgccgccggc catccccata ttgccgagca gcagctggat catcgccatc gtacgaatgt
     4441 tttgcgcacc aacggagtgt tgcgtccagc cgagggcata caggaacgag gcagttttat
     4501 cgtgagcact ggtttctgcg atgtattcgc agactttcag gaacgcgtct tttggcgtac
     4561 cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac gtgctgtttc agcaagttcc
     4621 acacgcagcg cggatgttgc agcgtggtat cgcgtttggc gaagccgttt tcgtccagtt
     4681 cataagtcca ggaggattta tcgtacttgc gtttttccgc gtcgtagccg gtgaacaggc
     4741 catcttcaaa gccgtaatcc tcacgcacta tcaggctggc gttggtatag gcttcggtgt
     4801 attcgcggtt gaatttttca ttgttcagca ggtacagcaa tacgcctgac aggaaagcaa
     4861 tgtcagtacc ggaacgaata ggggcatagt agtcagctac cgccgccgta cgcgtaaagc
     4921 gaggatcgat cacaatcagc ttcgcgccgt tgtgaatttt ggcttccatc gcccagcgga
     4981 acccgaccgg gtgagcttca gcggcgttac cgcccatcac cacgacgagg ttggcgttct
     5041 tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca agacttgcta
     5101 ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc gagcataccc agcgcgcggg
     5161 agaatttttg cgttaaatag ccggtttcgt tactcgacgc ggaagcacac agcatcccgg
     5221 tggagagcca gcggttaaca gtcacgcctt cggcgttttg cgcaatgtag ttagcatcgc
     5281 ggtcttcttt catcagtttg gcgatgcgat caaacgcctc ttcccaactg atttgctgcc
     5341 atttatcaga acctggcgca cggtattccg gaaacttcag acggctttcg gagtggatga
     5401 aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg gttgaccggg tgatccggat
     5461 cgccttcgat atggaagata gatgctttgg cgttttttgc tccgtcaccg aggctgtaca
     5521 tcaacagccc acagcctacg gaacaatagg tgcaggtatt acgggtttcg cgggtgcgca
     5581 gcagtttata ctgccgggtt tccgcgagcg ctacgctggg tgcaaaaccc agtgccgctg
     5641 ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa ctgccttctg ctgacctgca
     5701 tggattgctc cttgtttcga cattgtcacg tcccatttac attcgcttgc tgcgtgtgca
     5761 gggagtggga gttatttttc tttgcggaag gggccgcaaa ggtccagaat tggctcaatt
     5821 tccctccatc caggaagggt ttgtaacaga ataccataat gttggtgtgt gtgttcttat
     5881 ctggttaaga gaaagtgaaa aaaacacagc aaaaagaaat cgaaaatgtg acaaatatca
     5941 caggtgttcg tcaaattgag ttatggcggc gtgatgattt acaacaccca cggttagatg
     6001 aggtcgcgga agaagttccc gttgcgctgg tctacaacgg catttcgcat gtggtgatga
     6061 tggcgtcgcc caaagacctt gagtactttg cgctcggttt ttcgctttcc gaggggataa
     6121 tagaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc ttcctgtaat ggtcttgaag
     6181 tacaaattga gctttccagc cgccgcttta tggggttgaa ggagcgccgc cgggcgctgg
     6241 cgggacgtac gggctgtggc gtatgcggcg tggagcaact taatgacatc ggaaaaccgg
     6301 tgcagccgct accgttcacc cagacgtttg atctcaacaa actggatgat gcattacgtc
     6361 atctcaacga ttttcagcca gtggggcaac tgactggttg tactcacgcc gctgcctgga
     6421 tgttgccatc tggcgaactg gtcggcgggc atgaagacgt gggtcgccat gtggcgctgg
     6481 acaaactgtt aggccgccgg tcacaagaag gggaaagctg gcagcaaggt gcggtactgg
     6541 tttccagtcg tgccagttat gaaatggtgc aaaagtcggc gatgtgcggc gtagagattt
     6601 tgtttgcggt gtctgccgcg accacgcttg ctgtagaagt ggctgagcgt tgtaatctga
     6661 cgctggtggg tttttgtaag ccgggtcggg caacggttta tacccatccg cagcgtttga
     6721 gcaattaatt aaaataagtc ctgcgatata tattaaattg caggacttat tcatttcgtg
     6781 aattttatta ttttatttat aa
//
LOCUS       DAZFWL020000203         6470 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFWL020000203 DAZFWL020000000
VERSION     DAZFWL020000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.204"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..275
                     /locus_tag="ACHKNP_RS23010"
                     /old_locus_tag="pgaptmp_004602"
     CDS             <1..275
                     /locus_tag="ACHKNP_RS23010"
                     /old_locus_tag="pgaptmp_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4706628.1"
                     /translation="ARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSH
                     SSEYYREIVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            298..1260
                     /locus_tag="ACHKNP_RS23015"
                     /old_locus_tag="pgaptmp_004603"
     CDS             298..1260
                     /locus_tag="ACHKNP_RS23015"
                     /old_locus_tag="pgaptmp_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4706629.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PEPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            1267..2008
                     /locus_tag="ACHKNP_RS23020"
                     /old_locus_tag="pgaptmp_004604"
                     /pseudo
     CDS             1267..2008
                     /locus_tag="ACHKNP_RS23020"
                     /old_locus_tag="pgaptmp_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2034..2795
                     /locus_tag="ACHKNP_RS23025"
                     /old_locus_tag="pgaptmp_004605"
     CDS             2034..2795
                     /locus_tag="ACHKNP_RS23025"
                     /old_locus_tag="pgaptmp_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4706630.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGEAGVTEGQALQPEAPDVVTEEVAPKVTADMMVEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMSGGNLPADPKAAPVTKNDGKA
                     FPQPAGAALPVREAATQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKCRDILRDITATREQQR"
     gene            2828..3109
                     /locus_tag="ACHKNP_RS23030"
                     /old_locus_tag="pgaptmp_004606"
     CDS             2828..3109
                     /locus_tag="ACHKNP_RS23030"
                     /old_locus_tag="pgaptmp_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4706631.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            3106..3333
                     /locus_tag="ACHKNP_RS23035"
                     /old_locus_tag="pgaptmp_004607"
     CDS             3106..3333
                     /locus_tag="ACHKNP_RS23035"
                     /old_locus_tag="pgaptmp_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706632.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            3326..3637
                     /locus_tag="ACHKNP_RS23040"
                     /old_locus_tag="pgaptmp_004608"
     CDS             3326..3637
                     /locus_tag="ACHKNP_RS23040"
                     /old_locus_tag="pgaptmp_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706633.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            3765..3983
                     /locus_tag="ACHKNP_RS23045"
                     /old_locus_tag="pgaptmp_004609"
     CDS             3765..3983
                     /locus_tag="ACHKNP_RS23045"
                     /old_locus_tag="pgaptmp_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HGU4706634.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            3985..4542
                     /locus_tag="ACHKNP_RS23050"
                     /old_locus_tag="pgaptmp_004610"
     CDS             3985..4542
                     /locus_tag="ACHKNP_RS23050"
                     /old_locus_tag="pgaptmp_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4706635.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            4833..4988
                     /locus_tag="ACHKNP_RS23055"
                     /old_locus_tag="pgaptmp_004611"
     CDS             4833..4988
                     /locus_tag="ACHKNP_RS23055"
                     /old_locus_tag="pgaptmp_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4706636.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            5108..5452
                     /locus_tag="ACHKNP_RS23060"
                     /old_locus_tag="pgaptmp_004612"
     CDS             5108..5452
                     /locus_tag="ACHKNP_RS23060"
                     /old_locus_tag="pgaptmp_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HGU4706637.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            5574..5846
                     /locus_tag="ACHKNP_RS23065"
                     /old_locus_tag="pgaptmp_004613"
     CDS             5574..5846
                     /locus_tag="ACHKNP_RS23065"
                     /old_locus_tag="pgaptmp_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706638.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            5848..>6470
                     /locus_tag="ACHKNP_RS23070"
                     /old_locus_tag="pgaptmp_004614"
     CDS             5848..>6470
                     /locus_tag="ACHKNP_RS23070"
                     /old_locus_tag="pgaptmp_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4706639.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSVYRESDIIPG"
BASE COUNT         1695 a         1414 c         1892 g         1469 t
ORIGIN      
        1 tggcccgtaa tacccagaaa attttccgtt ggctggataa agacacccct gatgctgttg
       61 aaaaaatgca ggctctgtta ccggcgatcg aaaaggcgat gccgcctttg ctggtggccc
      121 gtatgcgcag ccacagttct gaatattacc gtgagatcgt cgaacggagg gatcggctgg
      181 tgaaggatgt cgatgatttt gttgcgtcag cggttgtttt gtatgaccag atgaatcgcg
      241 gcggcccggc agggaatgct gtggtgatgc actaaaagca cggtgttcgg gggttttatg
      301 agcagcaagc ttcatggtct tgtctgggaa gggtgcgcct tcaccggcat gatcttatcc
      361 agggtggcgg ttatggcccg tcttgcagac tacagcaatg acgagggcgt gtcatggcct
      421 gccattgaaa ctatccggcg tcagatcggt gcaagaagtg aatccacagt gaaatcggct
      481 attgcagaac tggcgaaaga gggctggctg acgaaggaag agcgtaaggt cggtgggcgt
      541 aatgtaagca atatctatcg gcttaatgtg gaaaaactcg aagcagctgc ggcggcggcg
      601 cgtgagtcat ataaaccgaa aagaaaaatt agcccggcaa aaaatgaccc gttaacagtt
      661 gacccgtcaa atattgcccc ctcaacggtt gacccgtcaa attttgatgg atcaactgtt
      721 gataacaaac tgccgattag gggggcgatg attgaccccg atccgtcagt attaaaacct
      781 gagccgtcag ataaaagatc ttcttgtccg gacgcttcgc aaccggaccc gcagacggct
      841 gaacaggatt ttttaacccg acaccctgac gcggttgtgt tcagtgcgaa aaaacgccag
      901 tggggaagtc aggaagattt ggtgtgcgca cagtggatct ggggacgaat cgtgagtctt
      961 tacgagcagg cggccagcga tgatggcgag atcacgagac cgaaagaacc caactggact
     1021 gcatgggcca atgacgtgcg gacaatgcgg atgctggatg gcagaactca cagacaaatt
     1081 tgtgaaatgt ttgggcgtct ccagcgggat tcgttctggg taaaaaacat catgagtccg
     1141 gcaaaactcc gggaaaaatg ggatgaactg gttatccgcc tggggcgttc gcccgcgcag
     1201 cgttgcgtga atcacatttc tgaaccggac actgaaatac cgccgggatt cagggggtga
     1261 cgtgtcatga aaaacattgc ggcagttggg gttcttgaac gtattcgcag acttgcacca
     1321 caggggggcg gttccaccgt accggacggt ggaggagtgg cgggaatggc aacttgctga
     1381 aggacgaaaa cgcagcgagg agattaaccg cctgaatcat caggtgcggg ttgaaaaaat
     1441 cctgaaccgt gcgggcatcc agccgcttca caggaagtgc tcattcggga actaccgggt
     1501 gcagaacgac ggtcagcgcc atgctctgag ccaggcgaaa tccattgccg atgaattgat
     1561 gaccggatgt acaaacttcg tgttcagcgg taaacctggt accggtaaaa atcacctggc
     1621 agcagcgatt ggcaatcggc tgatggcgaa ggggagaagc gtgattatcg tcaccgtgtc
     1681 cgatgtcatg agcgtgttgc atgacggcta cgacaacggc cagtccgggg aaaaattttt
     1741 acaggagctt tgtggagttg accttctggt ccttgatgaa attggcatgc agcgggatac
     1801 gcgcaacgag caggtcacgc tgaaccagat agtcgaccgc agaacggctt cgatgcgtag
     1861 tgtcggaatg ctgacgaacc tgaatcacgc agcgatgagc acactcctcg gagatcgggt
     1921 gatggaccgt atgaccatga atggtggtcg ttgggtgaat tttaactggg agagctggcg
     1981 gtcaaacgtt ggacgtcagg gtatgtgaga atttttgacg aggtaaattt tcgatggaaa
     2041 ctgtattgca tgcactgaaa gcgatgggaa aagccaattc tgttgaactg gcggcgcggc
     2101 ttgatatcag ccgtgaagaa gttcttaacg aactgtggga actcaaaaaa aatggcgttg
     2161 ttgataaaac gggtcacacc tggtttctgg ctgtcgaagg tgaagccggg gtaaccgaag
     2221 ggcaggcact acaacctgaa gcgccggatg tggtaaccga agaggtcgct ccaaaagtta
     2281 ccgcagacat gatggttgag tttatcggtc aggatggtgc taaaacgtgt gaggaactgg
     2341 cgggtaagtt cggcgtcagt actcgcaagg ttgcttccac gctggcggtg gtaaccgcaa
     2401 cggggcggct ggcacgcgtt aatcagaacg gtaaatttcg ttactgcatg tcggggggta
     2461 atttaccagc agatccgaaa gccgcgccgg taacgaaaaa tgatggtaag gcctttcctc
     2521 agccagcagg tgctgcgtta ccagtccggg aagccgcaac acaggaagaa ataaaaacgg
     2581 aaagtgtggc ggtcacagtg cagtcacagc cgtcgttcac cagaaagcat ccggatggtc
     2641 tgattttacc atcgctgcat gtggctaacc gcgagctgcg ccgggcaaaa ggtcaggttc
     2701 agaagtggga gcgagtctgc gccgcgctgc gggagctgaa caagtgccgg gatattctcc
     2761 gggatattac cgccaccaga gaacagcagc ggtgagtggg tggaagacgt ggtgccgggc
     2821 ggaaatcatg atactccggc agtgtgcggg aacgatgaag gtaaaaagcg ttggcgcact
     2881 tatcggacga actgaagcgg cagtgagaac gaaggcacgg gagctgggca tcagcatgat
     2941 gttacgtggt gattttcacc cgtcggcaaa atattctcag cgtgatattg agctggcgcg
     3001 gcaactgcat cagagaggca tgcaaagaag ggaaattgcc agaaaattag gcatgccgct
     3061 gcgcatagtg aataactacg tttatttcga caggagggtg tctgcgtgaa aatcctgtat
     3121 caggattacg gcccggtggg gcaggtggtt atcagcagta ctgtaatgga gtttcggaag
     3181 cataaccgtg tggtggatgc tgtgctgtta acctgtccgg ggatatcggc gagtcgtgca
     3241 ggtgtgttta ttatgaagac gaaattatat ggcagtaagg cgtggataaa gaaggcgtat
     3301 cgtgtagcgt tgcaggaggt taacagtgag tgaaattaaa gaaatgccgg tagttcgtga
     3361 cggatatggc tactggacac atcctgaata tgaaaaattc tgtgatggtc gggaatatat
     3421 ttcaacggaa gagtttaacg cctggatgga ggaaaataat cttcaatacg tcctctgctt
     3481 cagagatgaa ggatgtgctg accttgatgc gtgtgatgct gatatttctg catgggaacc
     3541 ggaacgacca gagggcgatg gctggtttat tggttccatt catgatacgg aagatggccc
     3601 ggtttgtgtc tggttgcgaa ataaggctga agcataaagg cgataaacca actaacaact
     3661 aaatactgaa gatttaaatc agaaacgatt tttattaaat ccttaaccgg agggattcct
     3721 gcaccctcag aacatcagga ggccgcccga aagggcggta agaaatgaaa cattatttag
     3781 aaaaaaatta cccacgaaag agcagaacaa cagagtttct gtttttcatt ctgtttatag
     3841 tgttgatgat accgatatcc ccgctattac tggtctggat aattggaagg acatttgaac
     3901 cagttattga gctatatacc gatgtgacat gggaatcatt cagcgcactg cacaataaaa
     3961 ttaatccgta taaggaaaac tgatatgagc actattacca gagaacgcgc ggagattaaa
     4021 tcatacatca caggcttcct gagcgactcg gcgcacgata acaagtcttc agacagcctg
     4081 ctggctaatg tgtttcgtat cgcgctggca tcactggaag cagagccgat agcaatggta
     4141 gtgcctgatg aaatggattt gcttacctgc catctcgacg gtgtaactaa aacatatgct
     4201 gatggctgga acgcctgccg cgtcgccatg cttcaggccg gaaactttcg ggaaaataag
     4261 aattcgtcaa ccaacaattt tcgggaaatc tcggaaacgt caaccagatc tccgataact
     4321 ctggatggct ggataagctg tactgagcga atgcctgaaa agagccagaa cgtgcttatt
     4381 tcgatgaata tcgatagcga ggctgggcca ttaatatatt ccgcacgcta tctcggaggc
     4441 acgttccggc gcggaggtat agcagttagt ccgggtaatg atcttaggca agcaacccac
     4501 tggatgtcgc taccagaacc gccgcaggag gtgaatcaat gacctggcct gaagcattca
     4561 caacggtagg aattgcgatg gcggtggcgc tggtggtgta ttcgatttgc cgctggggat
     4621 aaaaacggtt tgcgggaaaa ggatagttaa gtagaattgc agcgggtgct tgaggctatc
     4681 tgcctcgggc atgaacacca acggcagata gagaaaagcc ccagttaaca ttacgcgtcc
     4741 tgcaagacgc ttaacattaa tctgaggctc aatccatgct gaacacatgt aggttagcct
     4801 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     4861 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct
     4921 gcgaggtgcg aatccgaacc ggtcagacgg aggtcgctgt cttcgtagac tacgaatctg
     4981 agaagtaaga gaccaggcgg gggagtaatc tcccgccacc tctgatgtgt caggcatcct
     5041 caacgcaccc gcgctttacc atactgaaaa tgctgtttga atgttcatct ctgaaagagg
     5101 actatgaatg aaaaaggtat tgattgcagc acttatttcc ggtgtgtctt ttggcgcttt
     5161 tgcacagcag ggtggtttcc aggggccaga agcagagcgt tcaacagtag cgcaggcaaa
     5221 agaactgaag gatgatgcat gggttatcct tgaagggagc atcgttaaaa aagtgggtga
     5281 tgaacgttat gagtttcgtg acaatagcgg gacaattgtc acggatattg atgacagcgt
     5341 atgggccggg cagaatgttt ctccgaaaga caaagtaaga attgagggtg aaattgataa
     5401 agacctgagc agtgttgaag ttgatgtaaa ggcactgaaa ttattaaagt aaccgcccct
     5461 gcttgttaag cccgtcttac tgacgggttt tctgtttgta cattccggcg tattgcctta
     5521 caattcgcgc agtcagcctg aacaactgac acctgctgtc accggagaat ccgatgacac
     5581 aacacataaa atcccacaat tctgaagccg acccggaaat taagcagggg aggcgttttc
     5641 gtgcgcctca gtatggctgg tttcactatc tgttctgtac gatcgatgag gcagatatgc
     5701 ttcaagaggc gtatctgcgt cgcggtgtcc gtgtggagcg gagtctgaac gctgatcgtc
     5761 tgacctggac cgtttctgta tatcttcctg ttcgtgcaca tctgccacgg acacatgcct
     5821 gctaccgtca gcgcgtctgg aggtaatgtg cgggtattac ttcgacctgt tctggttccg
     5881 gaactcgggc tggtggtcct taagcccggt cgtgaatcat tgccagtttt tcatcgcggc
     5941 agggtgctgg tggagccgga accgaaaaac atgcgggcgc tgccatctgg agcggttcct
     6001 gctgttcgcc agccgctggc ggaagataaa tcactgctgc catttttcag cgatgagcgg
     6061 gtgattcgtg cagctggcgg cgctggtgca ctgtctgact ggttattacg tcacgtgaaa
     6121 tcctgccagt ggccacacgg cgattatcat cacagcgaaa ccgttattca cagttacggt
     6181 gctggcgcaa tggtgttgtg ctggcactgc gacaaccagc tgcgcgacca gacctccgaa
     6241 tcacttgagc aacttactca acaaaatctg acagcctgga tgattgacgt catacgccat
     6301 gtaatgaatg gcacgcagga gcgggaatta tcgctggctg aattatcctg gtgggcagtc
     6361 tgcaatcagg tggtggacgc attacctgag gcagtatcgc gtcgctctct gggattaccg
     6421 gcggaaaaaa tccgctccgt ataccgtgaa agcgacatca taccgggaga
//
LOCUS       DAZFWL020000204         6411 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFWL020000204 DAZFWL020000000
VERSION     DAZFWL020000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.205"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(57..443)
                     /gene="ridA"
                     /locus_tag="ACHKNP_RS23075"
                     /old_locus_tag="pgaptmp_004615"
     CDS             complement(57..443)
                     /gene="ridA"
                     /locus_tag="ACHKNP_RS23075"
                     /old_locus_tag="pgaptmp_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HGU4706640.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(516..977)
                     /gene="pyrI"
                     /locus_tag="ACHKNP_RS23080"
                     /old_locus_tag="pgaptmp_004616"
     CDS             complement(516..977)
                     /gene="pyrI"
                     /locus_tag="ACHKNP_RS23080"
                     /old_locus_tag="pgaptmp_004616"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HGU4706641.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(990..1925)
                     /gene="pyrB"
                     /locus_tag="ACHKNP_RS23085"
                     /old_locus_tag="pgaptmp_004617"
     CDS             complement(990..1925)
                     /gene="pyrB"
                     /locus_tag="ACHKNP_RS23085"
                     /old_locus_tag="pgaptmp_004617"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HGU4706642.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(1929..2063)
                     /gene="pyrL"
                     /locus_tag="ACHKNP_RS23090"
                     /old_locus_tag="pgaptmp_004618"
     CDS             complement(1929..2063)
                     /gene="pyrL"
                     /locus_tag="ACHKNP_RS23090"
                     /old_locus_tag="pgaptmp_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HGU4706643.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(2344..2739)
                     /locus_tag="ACHKNP_RS23095"
                     /old_locus_tag="pgaptmp_004619"
     CDS             complement(2344..2739)
                     /locus_tag="ACHKNP_RS23095"
                     /old_locus_tag="pgaptmp_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU4706644.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(2870..3583)
                     /gene="bdcA"
                     /locus_tag="ACHKNP_RS23100"
                     /old_locus_tag="pgaptmp_004620"
     CDS             complement(2870..3583)
                     /gene="bdcA"
                     /locus_tag="ACHKNP_RS23100"
                     /old_locus_tag="pgaptmp_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4706645.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3654..4247
                     /locus_tag="ACHKNP_RS23105"
                     /old_locus_tag="pgaptmp_004621"
     CDS             3654..4247
                     /locus_tag="ACHKNP_RS23105"
                     /old_locus_tag="pgaptmp_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU4706646.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKVGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            4392..4844
                     /gene="tabA"
                     /locus_tag="ACHKNP_RS23110"
                     /old_locus_tag="pgaptmp_004622"
     CDS             4392..4844
                     /gene="tabA"
                     /locus_tag="ACHKNP_RS23110"
                     /old_locus_tag="pgaptmp_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HGU4706647.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            4967..>6411
                     /locus_tag="ACHKNP_RS23115"
                     /old_locus_tag="pgaptmp_004623"
     CDS             4967..>6411
                     /locus_tag="ACHKNP_RS23115"
                     /old_locus_tag="pgaptmp_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706648.1"
                     /translation="MAKISDLNCYQHMASADNVKPKNEVLNTWCVGMNDFARNAEGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMNF
                     VMNNPEGERADSSKVIIEISLSTTTTMGTRQGHTAIIFPQSDFLSFRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKNMALLLNNHQLNWKDAIDYNELLEKPLASTLEKIKKEH
                     LLLMPHVCDDTIPYLLGEGGILEEINDLKALHDTRIASGTEGNDEINNIKINLSNILI
                     DSLDDAKVNLEKVIDSMLKTFLKLPYINDVKILEWCFNQSMQYSDDTTKIKHARSVID
                     HIDFNCDQSKIAETLFFNLNKEPYKNSPELQELIWEKLVVYVNDFNLSNQEKSRLILR
                     LFDDVKLLFNEVPVSILVNDIFLKDFFMKQPDFAKWYFYQLIKKYEGEQLYLNELGYV
                     YGNEEKTNEIVKKLPGYVIKIFEEKMGNELK"
BASE COUNT         1734 a         1400 c         1532 g         1745 t
ORIGIN      
        1 ttttgcgttt gtcatcagat ttaatccggg catgatagcc cggatttcca tcaagattag
       61 cgacgaacag cgatcgcttc gatctcaatt tttacgtctt tcggcagacg ggcaacttca
      121 acgcaagaac gtgccgggaa ggtggcattg tgttcggtga agaaggcttc gtaggtagca
      181 ttaacggttg cgaagtcgtt cagatctttc acaaacacgg tggttttaac gatgtcgccc
      241 actttcaggc ctgcggcttc gacgatcgct tttacgttat ccagcgactg acgtgcctgt
      301 gcagcgacgt ctgccggtac ttcgcccgtt ttcggattta ccgggatctg gccggaggtg
      361 atgatcatat tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt
      421 tccgtcgcga tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc
      481 attataggga gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg
      541 gaaaactctt tttcacagta tttgcatttg agcgcgatgt cattggcgcg ttttcgcacg
      601 gcaaagctgg aagaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc
      661 acattgtcga tgcgctccgg cagactgggg cgcgatttac ccaccacttc atagttgtcg
      721 atacggttaa ccgtggcttg cggcgcatac aatgccagtt gatctacctg atcttcactc
      781 aaaaaggtat tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaggttc
      841 agaccaatgg tgatgcgctg atccgtttcg gtcagcttga acagactcaa cagcttaaaa
      901 ccgatctggg cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat
      961 ttattatcgt gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt
     1021 gccagtaacg cctggcgagc gaaaatcccg ttgcctgcct gctggaagta ccaggcgtgt
     1081 ggcgttttat caacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc
     1141 atattggctt tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg
     1201 gcgtactcgg acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact
     1261 tccgccatca cttcttcaat agagctgtgc agactccatg cgatcccttt ttcatcgagc
     1321 atatccagaa tgtattgcgg cattgccagc gcgtccggcg cgatgaagta aaaacggttg
     1381 ccatcgaact tcgctaacgc ctgagtcagg gagtgaacgg tgcggccata tttcaggtca
     1441 ccaaccattg cgacgtggag attgtccaga cgcccctggg tttcctgaat agtgaataag
     1501 tccagcaagg tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca
     1561 ttgccggaaa actcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc
     1621 gcatcgacgt aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ctttttaccc
     1681 agtgatgtat tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatagat
     1741 gtttcgaaag agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg
     1801 tgcttcaaca gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga
     1861 ttaaggtcat cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta
     1921 gccatctttt atctcctgac gcctgggcaa aaaaaagccc ctcgattgag gggctgggaa
     1981 tgggtgatca acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca
     2041 aaatgtcgaa cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata
     2101 gccagaagcg gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat
     2161 taaaagtcaa aatgtatgca tttttatttg ctttgattct ggaggagaac tgtcgttcgg
     2221 tatcaataat tgcagacatt tcctgctttt ttctttttca ctttcacgca atcagatatg
     2281 cattttattc cattcatccg acttataggg cggagtgtga aaacgaacgg ctaacactat
     2341 tgcttacagc tcagggatgc gcgctatcac tttaatttca aaatcaaagc ctgccagcca
     2401 ggtaacgccc accgccgtcc agtttggata aggaggggcg ctaaatattt catttttgac
     2461 cgtcatgatg tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat
     2521 atcgtcaaaa gtgcatcccg cagcagccag ggtcgcatgc aaattatcaa atgccagtct
     2581 gacttgttgc tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga
     2641 aacaaacagc aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga
     2701 atgtcggcca gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac
     2761 gacatgaaca gaagatttct ctttaccggg agccgctttt agcggacgac gtgagtaaac
     2821 aaaacccaga catcatggat aatggctggg cttaattgag cgtagtcggt tatgcgccaa
     2881 acgcgccatc aatggtatgc atcgcgccgg taacaaaact ggcttctggc cctgctaacc
     2941 atgcgaccat accagcgacc tcttccggtt gcccatgtct tttgatagcc atcaaactat
     3001 gcaacatatc gcgcattggc ccgttggcgg gattagcgtc ggtatcaatt ggccctggct
     3061 ggacgacgtt aatggtgatc ccacgcggtc caaaatcacg ggccagcccg cgcgccatgc
     3121 cttgcagggc agatttgctg gcggcataag cagccatgcc tgcaacaggc atacgatcgc
     3181 cattcacgga gccgatgatt aagatgcgcc cgccttcggg catctgccgg gcggcttcaa
     3241 cagaggcatg ataaggagca tgaatattga ttttgaaaag gcgatcaata tcgtcggcat
     3301 ttaattccag ggcatcgcca aagacgccaa tacctgcatt taccaccagg atatccaatg
     3361 cgccgctctt acgaacgaca tcaatgacag cgtctctgtc agcactatct gtaaatactg
     3421 ctgtcgctcc agtctcttgt gccagatgtt cagcggcatc tttcgacccc gcataggtga
     3481 atcgtacatt ggccccatca gtgacgaaac gacgtacgat agcggcaccg attccacgac
     3541 tgccaccgag gatgagaact gtcttacctg taaaagcgcc cataaggact ccttgattta
     3601 ttatgtaaca tgcattacaa aactgtttta actttctgtc aacaagtttt gtaatggtca
     3661 ctaaaaaaca atctcgcgtt ccaggtcgtc ccagacgttt cgctcctgag caggcagtct
     3721 ctgcggcaaa agtgcttttt caccaaaaag gtttcgatgc tgtcagtgtt gctgaagtta
     3781 ctgattatct tggtattaac cccccgagcc tctacgcagc ttttggcagt aaagttgggt
     3841 tatttagccg tgtactcaat gaatatgtcg gtacggaagc tattccgctt gccgatattc
     3901 ttcgtgatga tcgtccggta ggcgagtgcc tggttgaggt attaaaagaa gcggcgcgca
     3961 gatatagcca aaacggcggc tgcgctggct gtatggttct tgaaggtatt catagtcatg
     4021 atccacaagc gcgtgatatt gccgttcaat attatcacgc cgcagaaacg accatttatg
     4081 actatatcgc caggcggcat ccacaaagcg cacaatgtgt gactgatttt atgagtaccg
     4141 tgatgtcagg gctttctgcg aaggcacggg aggggcactc catcgaacaa ctctgtgcaa
     4201 cagctgcact ggcgggggaa gcgataaaaa ctattctcaa ggagtgatgc tggccttgac
     4261 ccgaaaggcg ggaacgcgcc tgccgataag ttgtgataag acaataattc acgcgttaag
     4321 gctagcggaa ttgatcatct tttcgtataa cggtggaaat gaaacattgt tttaattaag
     4381 gagtggaaaa gatgatcatc ggaaatattc ataatcttca gccgtggcta ccccaggagt
     4441 tacgccaggc gattgagcat atcaaagcgc acgttacggc agaaacgcca aagggtaagc
     4501 acgatatcga aggcaatcgt ctgttttatc ttatctcgga agatatgacc gagccgtacg
     4561 aagctcgccg tgcggagtat catgcccgct atctcgacat tcagattgtg ttaaaaggtc
     4621 aggaaggcat gaccttcagc acgcaacctg caggcacgcc ggataccgac tggttagctg
     4681 ataaagacat cgcatttttg ccggaaggcg ttgatgagaa aacagttatc cttaatgaag
     4741 gtgattttgt tgtgttttat ccgggggaag tgcataaacc gctgtgcgca gtgggcgcgc
     4801 cagcccaggt tcgcaaagca gtagtgaaga tgctgatggc gtgatgactt ttcgccgtaa
     4861 ataactccag gtttacggcg agtttgtgaa aagagcgttt tttgatattt ttttgtgagt
     4921 agaatttgta atgcttagac gttcttatta actcaaggag tccgtcatgg caaaaatatc
     4981 agatttgaat tgttatcaac acatggcatc agccgacaat gttaaaccaa aaaatgaagt
     5041 cttaaatacc tggtgtgtgg gaatgaatga ttttgctcgt aatgccgagg ggcaggatag
     5101 cagaaggaat attctatctc ctggagcatt tttggagttt ttggcaaaaa tatttaccct
     5161 gggttatgtg gattttagca aacgctccaa cgaagcgggt agaaatatga tggctcatat
     5221 tgagtcctca tcttatatca aaaataatga tggcagtgaa ataatgaatt ttgttatgaa
     5281 taatcctgaa ggtgagcgtg cggattcatc taaggtgatt atagaaattt cactttctac
     5341 taccactact atggggactc gtcaaggaca tacagccatt atatttccac aatctgattt
     5401 tttgagtttt cgttatgaag gtaagtcttt tgaaagaaaa gatgatagtt cattacatct
     5461 gattactaac aaggttctgg cgtgttacca acgcgaagct aacaagaata tggcgcttct
     5521 attaaataat catcagttga actggaagga cgcaattgat tataacgagt tattagaaaa
     5581 gccattagcc agtacattag aaaaaataaa gaaagaacat ctgctattaa tgcctcatgt
     5641 atgtgatgac acgattccat acttactagg cgaaggtggt atacttgaag aaataaatga
     5701 tctgaaagcg ttacatgata ccagaattgc tagtggtaca gaaggtaatg atgaaattaa
     5761 taacatcaaa attaacctgt ctaatattct tattgattcc ttagatgatg caaaagttaa
     5821 tctcgaaaag gtcatcgatt cgatgttgaa gactttttta aaattgccat atattaatga
     5881 tgtaaaaatt ctggagtggt gttttaatca aagtatgcaa tattctgatg atactacaaa
     5941 gataaagcat gcccgctccg tgatcgatca tattgatttt aactgcgatc agtctaaaat
     6001 agctgagaca ttatttttca acctcaataa agaaccctat aaaaacagcc ctgagttaca
     6061 ggaattgatt tgggagaagt tggttgtata tgtcaatgat tttaacttaa gcaatcaaga
     6121 aaaatcacgc ttaatactaa ggttatttga tgatgttaag ttgctattta atgaagtgcc
     6181 tgtcagcatt ttagtaaatg atattttttt gaaggatttt ttcatgaagc aacctgattt
     6241 tgcgaagtgg tacttctatc aattaattaa gaagtatgag ggcgaacagc tgtatcttaa
     6301 tgagttagga tatgtttatg gtaatgaaga aaaaacaaat gaaattgtaa aaaaacttcc
     6361 gggttatgtc attaagattt ttgaagaaaa gatgggtaat gaattaaaaa t
//
LOCUS       DAZFWL020000205         6333 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFWL020000205 DAZFWL020000000
VERSION     DAZFWL020000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.206"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            154..930
                     /gene="exuR"
                     /locus_tag="ACHKNP_RS23120"
                     /old_locus_tag="pgaptmp_004624"
     CDS             154..930
                     /gene="exuR"
                     /locus_tag="ACHKNP_RS23120"
                     /old_locus_tag="pgaptmp_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HGU4706649.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1170..1253
                     /gene="yqiM"
                     /locus_tag="ACHKNP_RS23125"
                     /old_locus_tag="pgaptmp_004625"
     CDS             1170..1253
                     /gene="yqiM"
                     /locus_tag="ACHKNP_RS23125"
                     /old_locus_tag="pgaptmp_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HGU4706650.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1275..1937
                     /gene="yqjA"
                     /locus_tag="ACHKNP_RS23130"
                     /old_locus_tag="pgaptmp_004626"
     CDS             1275..1937
                     /gene="yqjA"
                     /locus_tag="ACHKNP_RS23130"
                     /old_locus_tag="pgaptmp_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HGU4706651.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1941..2324
                     /gene="mzrA"
                     /locus_tag="ACHKNP_RS23135"
                     /old_locus_tag="pgaptmp_004627"
     CDS             1941..2324
                     /gene="mzrA"
                     /locus_tag="ACHKNP_RS23135"
                     /old_locus_tag="pgaptmp_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HGU4706652.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2471..2839
                     /gene="yqjC"
                     /locus_tag="ACHKNP_RS23140"
                     /old_locus_tag="pgaptmp_004628"
     CDS             2471..2839
                     /gene="yqjC"
                     /locus_tag="ACHKNP_RS23140"
                     /old_locus_tag="pgaptmp_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HGU4706653.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2877..3182
                     /gene="yqjD"
                     /locus_tag="ACHKNP_RS23145"
                     /old_locus_tag="pgaptmp_004629"
     CDS             2877..3182
                     /gene="yqjD"
                     /locus_tag="ACHKNP_RS23145"
                     /old_locus_tag="pgaptmp_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HGU4706654.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3185..3589
                     /gene="yqjE"
                     /locus_tag="ACHKNP_RS23150"
                     /old_locus_tag="pgaptmp_004630"
     CDS             3185..3589
                     /gene="yqjE"
                     /locus_tag="ACHKNP_RS23150"
                     /old_locus_tag="pgaptmp_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU4706655.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3579..3878
                     /gene="yqjK"
                     /locus_tag="ACHKNP_RS23155"
                     /old_locus_tag="pgaptmp_004631"
     CDS             3579..3878
                     /gene="yqjK"
                     /locus_tag="ACHKNP_RS23155"
                     /old_locus_tag="pgaptmp_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HGU4706656.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4064..4456
                     /gene="yqjF"
                     /locus_tag="ACHKNP_RS23160"
                     /old_locus_tag="pgaptmp_004632"
     CDS             4064..4456
                     /gene="yqjF"
                     /locus_tag="ACHKNP_RS23160"
                     /old_locus_tag="pgaptmp_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU4706657.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4526..5512
                     /gene="yqjG"
                     /locus_tag="ACHKNP_RS23165"
                     /old_locus_tag="pgaptmp_004633"
     CDS             4526..5512
                     /gene="yqjG"
                     /locus_tag="ACHKNP_RS23165"
                     /old_locus_tag="pgaptmp_004633"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HGU4706658.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5805..6170
                     /gene="yhaH"
                     /locus_tag="ACHKNP_RS23170"
                     /old_locus_tag="pgaptmp_004634"
     CDS             5805..6170
                     /gene="yhaH"
                     /locus_tag="ACHKNP_RS23170"
                     /old_locus_tag="pgaptmp_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4706659.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1616 a         1544 c         1559 g         1614 t
ORIGIN      
        1 acaatgacga tacttatttt ttaggaattt ataaatattc acaatattaa ttcaatacat
       61 taattcaatt accaaattta tatatgacca atggcatacc catgaataaa gtggtatgat
      121 aacttcgtcc gcattccact ccgggagacg catatggaaa tcactgaacc acgccgtttg
      181 tatcaacaac ttgccgctga cctgaaagag cgcatcgaac agggcgtata tctggtaggt
      241 gataaactgc ctgcagaacg ctttattgcc gatgaaaaga acgtcagccg tacggtagtt
      301 cgtgaagcca tcatcatgct ggaagttgaa ggctatgtcg aagtgcgtaa aggttcaggt
      361 attcatgtgg tttccaacca gccgcgccat cagcaggcgg ctgacaataa tatggaattc
      421 gccaattacg gtccgtttga gttgcttcag gctcgccagc tcatcgaaag taatattgcc
      481 gagttcgcgg caactcaggt aacgaaacaa gacatcatga aactgatggc cattcaggaa
      541 caggcgcgcg gcgaacaatg cttccgtgat tccgagtggg atttacagtt ccacattcag
      601 gtagccctgg cgacgcagaa ctccgccctg gcggccatcg ttgaaaaaat gtggacccaa
      661 cgtagccata acccgtactg gaaaaaactg cacgaacaca ttgattcccg taccgtcgat
      721 aactggtgtg atgaccacga tcaaatcctc aaggcgctga ttcgcaaaga tcctcatgct
      781 gctaagctgg caatgtggca gcacctggaa aacaccaaga tcatgttatt caacgaaacc
      841 agcgacgact tcgagttcaa tgccgaccgc tatctgttcg ccgaaaaccc ggtagtgcat
      901 ctcgatacgg ccaccagcgg cagtaaatga aatacctgtc cgacaggcgc tgcgacgcgc
      961 ctgtttgtct cgtcggtaag cgaaaagtat aaagtgtcag cctgtgtaaa tcctctcgcc
     1021 accctcccct gcattcagca aaatcagact ccacggacat gtaattttga taacgaacaa
     1081 cgttgacctt tgttacaatt agattcaatt tgaatttatg tttttgaatg ctttcttatc
     1141 tcacgattta acagggaata gttcaggctg tgttgatgta tcaaacccgc agaacatacc
     1201 aaaacagcaa taacattgcg gtagtgcatc ttttaaaacc agcgtggcgt taaccgattc
     1261 accaggaata atgaatggaa cttttgaccc aattgctgca agccctgtgg gcgcaggatt
     1321 ttgaaaccct ggccaatcca tcgatgattg gcatgttgta ttttgtcttg tttgtaattt
     1381 tgttccttga aaacggcttg cttccagcgg cctttttacc gggcgacagt ttactggtat
     1441 tggtcggcgt gttgattgcg aaaggcgcga tgggttaccc gcaaacgatt ctgctgctga
     1501 ccgttgccgc cagcctcggc tgctgggtca gctatattca ggggcgatgg ctgggcaata
     1561 cccgcaccgt acaaaactgg ctatctcatt tacccgcgca ttatcatcaa cgcgcacacc
     1621 atctttttca taaacacggt ttatcggcgc tgttaattgg tcgctttatt gcgtttgtca
     1681 gaacactgct gccgacgatt gccgggttat cagggctgaa taacgcgcgc tttcagtttt
     1741 tcaactggat gagcggtctg ctgtgggtat tgatcctgac aactctgggt tacatgctcg
     1801 gcaaaacgcc ggtattttta aagtacgagg accagctgat gtcatgcctg atgctgctcc
     1861 cggtggtgct gctggtgttt ggcctggcag gttctctggt cgtgttatgg aaaaagaaat
     1921 atggaaatcg ggggtaaggg atgcaaatac ctcgcatgtc gcttcgccag ctagcctggt
     1981 ccggcactgt tttacttctg gtcggcacgc tgttgctggc ctggtccgcg gttcgccagc
     2041 aagagtctac gctggcgatt cgtgccgttc atcaaggcac aacgatgcca gacggttttt
     2101 cgatctggca tcaccttgac gctcatggca ttcctttcaa aagtatcacc cccaaaaacg
     2161 acactctgtt aattacattt gactccagcg accagagcgc cgccgcaaaa gcggtcctcg
     2221 acagaacatt gccccatggc tacatcattg cgcagcagga caataacagt caggctatgc
     2281 agtggctgac ccggttacgg gataactctc atcgcttcgg ataacttcct gttatccgaa
     2341 acatatcact cactttggtg atttcaccgt aactgtctat gattaatgag gcggtggtta
     2401 acactaccgc ttcgttgtct ggacaatcgt tcctttgtaa taggtccgaa tcacaatgga
     2461 aggttcaaga atgaaatacc gcatcgcttt agctgtttct ctctttgctc ttagtgccgg
     2521 tagttatgcc actaccctgt gtcaggaaaa ggagcaaaat atccttaagg agatcagcta
     2581 tgccgaaaaa caccaaaacc agaatcgtat tgacggtctg aataaagccc tgagtgaagt
     2641 ccgggccaac tgttcagata gccagctgcg tgccgatcat cacaagaaaa tcgcaaagca
     2701 gaaagatgag gtggcggaac gccagcaaga tttagccgag gcgaagcaaa aaggcgatgc
     2761 cgataagatt gccaaacgcg aacggaaact ggcagaagcg caggaagagc tgaaaaagct
     2821 ggaagcgcgc gactactaac tcacaatagt cactacttac tcacctggag aaaactatgt
     2881 cgaaagaaca cactacggaa catctgcgtg ctgagttgaa atccctttcc gatacgctgg
     2941 aagaggtgct tagctcatct ggcgagaagt cgaaagaaga gttgagtaag attcgtagca
     3001 aagcggagca ggcactgaaa cagagccgtt atcgcctggg tgaaaccggt gatgccatcg
     3061 ccaaacaaac ccgtgtcgcg gcggcgcgtg ctgatgagta tgtgcgtgaa aatccgtgga
     3121 ccggcgtggg cattggcgct gcaatcggtg tagtgctcgg cgttctgctg tcgcgtcgtt
     3181 aattatggcg gacactcatc acgcgcaagg gcccggtaaa agcgttctgg gcatcgggca
     3241 gcgaattgtt tccatcatgg ttgaaatggt ggaaacacgt ctgcggctgg cggtggtgga
     3301 gctggaagag gaaaaagcta atctctttca acttttactg atgctgggcc tgacgatgct
     3361 tttcgctgca tttggtctta tgagcctgat ggtgctaatt atttgggcgg ttgatccgca
     3421 atatcgcctg aatgcgatga ttgccaccac cgtggtgttg ctgctactgg cactaattgg
     3481 cggtatctgg acgttacgta aatcgcgtaa atccaccttg ctgcgccata cacgccatga
     3541 gctggcaaac gatcggcaac tgctcgagga ggagtcccgt gagcagtaaa gtcgaacgtg
     3601 aacgacgtaa ggcgcaactg cttagccaga tccagcaaca acggctggat ctttccgcca
     3661 gtcgtcgtga atggctggag gcaacaggcg cttacgatcg tcgctggaat atgctgctaa
     3721 gtctgcgctc ctgggcgctg gttggcagta gcgtgatggc gatctggacg attcgccatc
     3781 ctaatatgct ggtccgctgg gccagacgcg gttttggcgt atggagcgcc tggcgtctgg
     3841 ttaaaacgac cctcaagcag caacagcttc gcggttaact tccctctggc cggagcaatt
     3901 ccggccttat ccctcaaatt tcttgaagat ttttgacagt tttccttgct aacaatcatc
     3961 attcaccacg tttatgattc tctccatcga cagcaacgac gctaataccg cgcccttgca
     4021 caaaaaaaca atcagcagcc tgagtggcgc agtggagagt atgatgaaaa aattagaaga
     4081 tgttggtgta ctggtagcgc gcattttaat gccgattctg tttattaccg caggctgggg
     4141 aaaaatcacc gcttactctg gtacccaaca atatatggaa gcaatgggtg tcccgggttt
     4201 tatgctgccg ctggtgattc tgcttgagtt cggtggtggt ctggcaatcc tgttcggttt
     4261 cctgactcgc accacagccc tgtttactgc gggctttacg ctgctgacgg catttttatt
     4321 tcacagcaac tttgctgaag gcgtcaactc gctgatgttc atgaaaaacc tgacaatttc
     4381 tggcggattc ctgctgctgg caattaccgg tccgggcgcg tatagcatcg accgcctgct
     4441 gaataaaaag tggtaagcac gcgctatact taacgataaa aaacgaggag gaagctcctc
     4501 gtttttgcta ttggaggaga gaaaaatggg gcaactgatt gacggcgtct ggcatgacac
     4561 ctggtacgat accaaatcta ccggcggtaa atttcaacgt tcagcttccg catttcgtaa
     4621 ctggctcact gccgatggcg cacctggccc cactggcaca ggcggtttta tcgcagagaa
     4681 agatcgttat catctctatg tttcactcgc ctgcccgtgg gcgcaccgca cgctgatcat
     4741 gcgcaaactc aaaggactgg aaccgtttat ttccgtttcc gtagtgaacc cgctgatgct
     4801 ggaaaacggc tggacctttg atgacagttt tccgggagca accggagata cgctctatca
     4861 acatgaattt ctgtatcagc tttatctcca cgccgatcca cactacagcg gacgagttac
     4921 tgttcccgtg ctgtgggaca aaaagaacca caccatcgtc agcaacgaat cagcagaaat
     4981 catccgcatg ttcaataccg cgtttgatgc gctgggcgca aaagcgggtg attactaccc
     5041 accagccctg caaacgaaaa ttgacgaact taacggctgg atttatgaca ccgttaacaa
     5101 cggcgtgtat aaagctggtt ttgccaccag ccagcaagcc tacgacgagg cggtggcgaa
     5161 agtgtttgaa tcgctggcgc gactggagca gattttaggc cagcatcgtt acctgaccgg
     5221 caaccagcta accgaagccg atattcgcct gtggaccacg ctggtccgtt ttgatccagt
     5281 gtatgtgacc cacttcaagt gtgataagca ccgcatcagc gattacctta atctgtatgg
     5341 cttcctgcgt gatatctacc agatgccggg aattgccgaa acagtcaatt tcgatcacat
     5401 ccgtaatcat tacttccgca gccataagac catcaaccct acggggatta tttcaattgg
     5461 tccatggcag gatctcgatg aaccgcatgg acgagatgtt cgcttcggtt aaaaaaaggc
     5521 accttacgag gtgcctctga taattagcta agtgcatgtt gttcatgtcg gatgcggcgt
     5581 gaacgcctta tcaaacctac aaaacattta tattcaatat gttgcaatga ccgcgcaggc
     5641 cagataagcg tagcgcatct ggcgattttg cgcttgtcat cagtcttagc gcgccttcac
     5701 agcactttat taattccttg aacgaatatt tactgccatt tcattcacgt ctattcttaa
     5761 tttgctgctt taaaaacaag tgattgagca aaattgaggc aaaaatggac tggtatctga
     5821 aagtactaaa aaattatgtc ggtttccggg gtcgtgcgcg gcgcaaagag tactggatgt
     5881 ttattctggt caacatcatc tttacgttcg tgctggggct gctggataaa atgttaggct
     5941 ggcaacgcgc tggtggcgaa ggcatcctga cgacaatcta cggtattctg gtgtttttac
     6001 cgtggtgggc agttcagttc cgccgcctgc acgacaccga ccgctcggcg tggtgggcac
     6061 tgttgttctt aatcccgttt atcggctggc ttattatcat cgtctttaac tgccaggcgg
     6121 gtacgccagg cgaaaaccgc tttggaccag atccgaaact ggaaccttaa aacaaatctg
     6181 ctcgccggat gtctgactca tccggctttt atcatcatgt tgatgaatga ataatttact
     6241 aatgtttatt taaaatattt caatgtcgtt atataaatta cctataaaaa acaaccatgt
     6301 atttatctta aatatcaaaa cctttataac atc
//
LOCUS       DAZFWL020000206         6251 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFWL020000206 DAZFWL020000000
VERSION     DAZFWL020000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.207"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            418..1434
                     /locus_tag="ACHKNP_RS23175"
                     /old_locus_tag="pgaptmp_004635"
     CDS             418..1434
                     /locus_tag="ACHKNP_RS23175"
                     /old_locus_tag="pgaptmp_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706660.1"
                     /translation="MSSVRTDKFIRYGFAVVAIVAILITTITTPNPTPYQYIVYRIVL
                     VLAAGCIGAVLPGFLEVKFNGWLRASGAVAIFAIVFFISPDLLQQNPESASKPVPPTV
                     IDAYAVNSSNRDRYLELFRTGQFGQRDKLRIGCVEWSESSCVAAGNFLRLLSEAGWEI
                     DSDAVFRMQPSVPVEGVSIVSRGDDLVGLPKVPPHMGRWSRSDISYEILTAAFKLMGV
                     PVYFSSDPTLQPGTTGVYFGPKPKLDAPVMQKDENVRRELLRYVGLGMTIQRACSQQP
                     VELCGGEIFSWETSVSSFLRQQRFNSDAVNRWLALPQVNAASATADMKIKLDLLMSFV
                     LGIE"
     gene            2078..4438
                     /locus_tag="ACHKNP_RS23180"
                     /old_locus_tag="pgaptmp_004636"
     CDS             2078..4438
                     /locus_tag="ACHKNP_RS23180"
                     /old_locus_tag="pgaptmp_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HGU4706661.1"
                     /translation="MKSMQSKTGSPLVRTEAELESRLTSALNIAFPNIPREDLIEQRH
                     FTVRLGHGTYKIDSAAHWKKYGRADVLIFHRERPLAVIELKREDLTLTHDDYEQAQSY
                     ANQLTPRPPLVVVTNGKDTRVYDSSNGQQWSGGQDASAAVNKMLANSAKLAAADMRWA
                     IEALMGRETNAWVPAVREETARLLIDITDQPGHSEHPFANNLLFPRKITSLVIESAVM
                     GTAFTIIEGDAQSGKSSCLREISLKTESSDLLAVLMLRGSGPGLFQALANLFAAEFEW
                     NLTSNDARNWLRRMSNCTEGPSLMLAIDDVEPGSQMATDLEELAGIRFGNRLVVVLTT
                     YHANALLKNPNGRTPSAIGSRSKVFKTSPMSLDEFKLAQQILSDQRIVFQQGAEYADD
                     YRSPWVLRTIYDDIVRNHQYQKTDLIAYLPPSPGMELIDAAQKSYESQYDLLRYYRVL
                     ARCALADTNSHSVELMFAKANGFVVRYDALSDEARGVVNELKHMGAVRIFRLSGWEDV
                     VVPTVPAAYLLELSDAVCDELVLRAEQDPQDAGAWLGERLDATYLGDMIGAQAIRRMA
                     AKERYFSFGIIQGLLSIEPYKEPIKNGLFTLAMPDNQQVNLKIEDGLAWISKHGDDAK
                     SVLVNLEDQIPKVISKSTSWMILGQLAKLPSAEVGDDDQRIDAYILLSIGRCPFPLIR
                     TNIEGLPYFEHNFGDQGDVLCLEKASIEVATQAMADLFSAPWLYADQWVDTAIATGSI
                     HLLHRLFAALNTVILRRIPVQSDWANEALNQRVSPALKEAIRSLSS"
     gene            4615..5754
                     /locus_tag="ACHKNP_RS23185"
                     /old_locus_tag="pgaptmp_004637"
                     /pseudo
     CDS             4615..5754
                     /locus_tag="ACHKNP_RS23185"
                     /old_locus_tag="pgaptmp_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5751..5906)
                     /locus_tag="ACHKNP_RS23190"
                     /old_locus_tag="pgaptmp_004638"
     CDS             complement(5751..5906)
                     /locus_tag="ACHKNP_RS23190"
                     /old_locus_tag="pgaptmp_004638"
                     /inference="COORDINATES: protein motif:HMM:NF015301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PapB/FocB family fimbrial expression
                     transcriptional regulator"
                     /protein_id="HGU4706662.1"
                     /translation="MTKGNISERDFDILIKLSGIHSEKAILALKKYYVDGVIRKIDPT
                     HVMWTRP"
BASE COUNT         1637 a         1370 c         1520 g         1724 t
ORIGIN      
        1 cttacttggc tttagtcccg gagtgttgcc aggagtatct ttattatctc gtcggcgttg
       61 atctggtttt cctgtcgatt tagcaattgg ttttggacca ggtttaagac ccatgatagt
      121 tatccttttg agatgtaaga gggaagccct caacattggt atattggata ctgagaaatt
      181 tttcaatggg agtgataaac ggccgaatga ttaactcgat attgtgatca acgtctgttt
      241 tttgcacact gtagactgac tcttggagca gaaggttgct ctgagcgagg aatggacagt
      301 tacactcatc accaacgcca atagcctcat tacgcgtata aagctgtatt aatcattggg
      361 gagcagttta atgcgggatt ttcgacttat ggacttgatt caggtgtcaa aattaaaatg
      421 agcagtgtaa ggacagacaa gttcattagg tatggttttg cggttgtggc gatagttgcc
      481 atactcatta ctactatcac aacccccaat cccacacctt atcaatatat agtttaccga
      541 atagttctgg ttctggctgc cggatgtatt ggtgcagttc tgcctggttt ccttgaagta
      601 aaatttaatg ggtggttaag agcatctggc gcagtggcga tcttcgctat agtgtttttc
      661 ataagcccag atttgcttca acaaaatcct gaatctgcaa gtaaacctgt accgcctact
      721 gtgattgatg catatgccgt taatagttcc aatcgcgaca gatacctcga attatttcga
      781 acaggacagt tcggtcagcg ggacaagctg cgcatcggtt gtgttgagtg gagtgaatca
      841 tcgtgcgtgg ctgctggtaa ttttttgcga ttgttaagcg aagctggctg ggaaatcgat
      901 tctgacgctg tattcagaat gcaaccatcc gtgccagttg agggggtatc gattgtaagc
      961 cgtggcgatg atctggtcgg tttgccaaaa gtaccacccc acatgggccg ttggagtcga
     1021 tcagacatta gttatgaaat tttgacagcg gcatttaaat tgatgggagt tccggtttat
     1081 ttttccagtg acccgacatt acaaccaggt actaccggag tgtactttgg accgaaaccg
     1141 aaactggatg caccagtcat gcaaaaagat gagaatgtgc gacgtgagct tctccgctac
     1201 gtcggtttgg gaatgactat tcagcgagcc tgctctcagc agcccgtcga actttgtggg
     1261 ggtgaaatct tttcctggga aacgtcggta tcttcgttcc taaggcagca gagattcaac
     1321 tccgatgccg taaacagatg gcttgccttg ccgcaggtta atgcggcttc ggcaactgca
     1381 gacatgaaaa taaagctaga tttgttaatg tcatttgttc tgggcatcga gtaactatac
     1441 ctcttgcgag gatgattgag cgtctaatgg accagcttag ccgttagcgc tatttgttta
     1501 cgtaagcctg tggaatccaa ctagtgcttc tacgatgcgt tgtttggtga aaaaaaagtg
     1561 ttgaagcact ggttttagac aactaaagcc acgatttgct catgcgtcga aaatgtagtc
     1621 gtctactgtg tagcacggag gcgatattcg tacgcttaca cttgccgata ggccactggg
     1681 gagtgttctt ggcactaagc tgccaggccc gaaggtctgc tatgagcgag gggctgacat
     1741 tcctattggc attcggtgtg agttatcggg ggcggtcatg cggcatactt ccgatacagg
     1801 cgtttccagc tctgcctgtt tcagggttat attaatctgc tttagaatta atcgtggctt
     1861 tagcatctgc acatcgtgct ggagaaacct gagtgaacta aatcatattc cctttcttca
     1921 gctaatcaat tgatagttat gcggaaaaag ttactttgct tgtcaataat ccatttcaca
     1981 tccgttaatc caatgctatg cgtcttcgca ttaatctgga aaggtagtaa gtgaaataaa
     2041 aacttagcca atgactaata tctgaggata ctttgtcatg aaaagtatgc aatcaaaaac
     2101 gggctctcca ctcgttcgaa ctgaagccga gctagaaagc cggcttactt cagcactaaa
     2161 catagcattt cctaacattc ctcgcgaaga tcttatcgaa cagcgccatt ttaccgttcg
     2221 gctaggccat ggaacttaca aaatagatag cgccgcacac tggaaaaaat acgggcgagc
     2281 ggatgttttg atctttcatc gggagagacc acttgcggtt attgaactga aacgtgaaga
     2341 cctgacactg acccatgatg attatgaaca ggctcaaagc tacgctaacc agttaacacc
     2401 acgtccgcct ctcgttgtag ttacgaacgg taaagatacg cgcgtttatg actcaagtaa
     2461 tggacaacag tggtcgggtg gacaggatgc ctcggcagcg gtaaacaaga tgctcgccaa
     2521 ttctgcaaag ttggcggcgg ctgacatgcg ctgggctatt gaagccttga tggggcgaga
     2581 aactaatgct tgggtgccag ctgtgcgaga agaaacagcc cggctgctga ttgatataac
     2641 tgatcagcct gggcattcag aacatccatt tgccaacaac ctactttttc ctcgcaaaat
     2701 cacgtctctt gtaattgaat ctgctgtgat gggtacagct ttcactatca ttgagggtga
     2761 tgcacaaagc ggaaaaagca gttgcctccg tgaaatttcg ctaaaaacgg aaagtagcga
     2821 tcttttggct gtcctgatgt tacgaggttc cggaccagga ctatttcagg ctctggccaa
     2881 tctttttgca gctgaatttg aatggaattt aacctcaaat gatgcacgta actggttaag
     2941 acgaatgtct aactgcactg aaggtccatc gcttatgctt gctatcgacg acgttgaacc
     3001 aggctcccaa atggctactg atcttgagga acttgcgggt atacgttttg gcaacaggct
     3061 ggtggttgtt ctgacgacct atcatgccaa cgcgttactg aaaaatccaa acggacgaac
     3121 tccgtctgca attggtagcc ggtcgaaggt atttaaaaca tcgcctatgt ccctagatga
     3181 atttaagctg gcgcaacaga ttctctcgga ccagcggata gtttttcaac agggagcaga
     3241 atacgccgat gactaccgct cgccttgggt tcttcgcact atttatgatg acattgtgcg
     3301 caatcatcaa taccagaaaa cggatcttat cgcctatctt cctccatcac ccggtatgga
     3361 acttatagat gctgcgcaaa agagctatga gagtcagtat gatcttcttc gttattatcg
     3421 ggttcttgca cgctgcgcac tagctgacac aaactcccac tcagtagaat taatgtttgc
     3481 taaagcgaat ggatttgtcg tgcggtatga cgcactttct gacgaagctc gtggtgtagt
     3541 gaatgagtta aaacatatgg gggcagttcg tatttttcgc ctttcaggat gggaagatgt
     3601 tgtggtgccg acagttcccg cagcttacct tctggaactg tcggatgctg tttgtgatga
     3661 actggtttta cgggcagaac aagaccctca agacgccggg gcttggcttg gcgaacggct
     3721 agacgccact tatcttggag atatgatcgg tgctcaagcc atacgccgta tggcagctaa
     3781 agagagatat ttcagctttg gaattattca gggccttctc agcattgagc catataaaga
     3841 acctataaaa aacggattgt ttactttagc catgccagac aaccaacaag tcaatctaaa
     3901 gattgaggat ggattagcgt ggatttctaa acacggggat gatgcaaaga gtgttctggt
     3961 gaatttagag gatcaaatcc caaaggtcat ttctaaatca accagttgga tgatactcgg
     4021 gcaactagcg aaactgccgt cagcagaggt gggtgacgat gatcagcgta tagacgccta
     4081 tattttactg agcattgggc gctgcccatt tcctctaatt cgcacgaata tagaaggatt
     4141 gccttacttt gagcataatt ttggcgatca gggtgatgtt ctttgcctag aaaaagcgtc
     4201 tattgaagtt gctactcagg cgatggctga tttattttca gccccatggc tctatgcgga
     4261 ccagtgggtg gatacagcta tcgcgaccgg ttctatacat ttactgcatc gtttgtttgc
     4321 tgctttaaac acagtgatat tgcgtagaat acccgtgcaa tccgactggg ctaatgaagc
     4381 acttaatcaa cgtgtctcac cagcccttaa ggaagccata cgatcattat cttcgtgact
     4441 aagcaatggg attttaatac ataacgagca tctgtccacg caagttaaca attgccttgg
     4501 ctcgatattt tggtttctag tgcatatatg ctgatcttct cttcgttgat ctgatcttac
     4561 ccaccaatag tggacacagg actaagtgag taaactctca accagaggtg actcatgaca
     4621 aaaccagtat caatcagcaa gaagccccgt gaacaacatt cgcctgaatt tcgtaacgaa
     4681 gccctgaaac tcgctgaacg catcggtgtg gccgccgcag cccgtgaact cagcctttat
     4741 gaatctcagc tttatgcctg gcgcagtaaa cagcagcaac aaatgagttc gtcagagcgc
     4801 gaaagcgaac tggccgctga aaatgtccgc cttaaacgac aactggcgga gcaggctgag
     4861 gaactggcca tcctccaaaa ggccgcgaca tacttcgcga atcgcctgaa atgaagtatg
     4921 tcttcatcga aaatcatcgg gcagagttct gcatcaaagc gatgtgtcgt gtacttcggg
     4981 ttgcccgcag cggctggtat gtctggctca ggcgtcgtca ccagatgagc cttcgccaac
     5041 agtttcggct cacctgcgat gccgctgtcc ataaggcatt ctttgaggca aagcagcgat
     5101 acggtgctcc ccgccttgct gacgaactgc cggagttcaa tattaaaacc attgccgcca
     5161 gcctgcgtcg tcaggggctg cgggcgaaag ttcagcccgg tcagctaccg tgcacatggc
     5221 ctgcccgtat tggagaatct gctggagcag gacttcagcg ccagaggccc gaaccagaag
     5281 tgggcgggtg acatcacgta cttgcgtacc gatgagggct ggttgtatct cgcagtagtc
     5341 atcgacctgt ggtcacgcgc cgtcattggc tggtcgatgt caccgcgaat gacagcacaa
     5401 ctggcctgtg atgcactaca aatggcgttg tggcggagaa gacgcccgga aagcgtcatt
     5461 gttcatacgg accgtggtgg tcaatactgt tcaggggatt atcaggcgct gctgaagcga
     5521 cacaacctgc gtggcagtat gagtgcgaaa ggctactgtt atgacaatgc ctgtgtggaa
     5581 agcttctttc attcgctgaa ggtggaatgt atccacgggg aacgctttag cagccgggaa
     5641 ataatgcggg caacggtgtt taattatatc gagtgtgatt acaatcgctg gcgtcgtcac
     5701 agtgcctgtg gcggtctcag cccggaacaa tttgaaaacc ataatctcgc ttagggccgt
     5761 gtccacatta cgtgggtagg atcaattttc ctgatgaccc catcgacata gtattttttc
     5821 aaagcaagaa ttgccttctc gctatgtatt cctgatagct tgatcaggat atcaaagtct
     5881 ctttcagata tattcccctt tgtcatgtgt cactccatga atcaaatttc atagtaatca
     5941 atctttcttt gatcgactta tagataataa gatcagaaat gataaaaaag taacgcatat
     6001 ttcttctcgt catgtctact tataaaattc aaatgaacta acctgaacta cttacactgg
     6061 aatatcatta tattggaata attataaaac tgaacaaaat ggtcaatgta atctggttaa
     6121 tttataaata tttacattgc gtgatgatat ttaattccag tgttaagtaa tgtcacatgc
     6181 ctattattgg ttactacttt gtaggtgttt tggttcggcg ttaatatcat tggtcataat
     6241 cacctgtgaa a
//
LOCUS       DAZFWL020000207         6140 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFWL020000207 DAZFWL020000000
VERSION     DAZFWL020000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.208"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..116)
                     /locus_tag="ACHKNP_RS23195"
                     /old_locus_tag="pgaptmp_004639"
     CDS             complement(<1..116)
                     /locus_tag="ACHKNP_RS23195"
                     /old_locus_tag="pgaptmp_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU4706663.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLLEA"
     gene            complement(149..448)
                     /locus_tag="ACHKNP_RS23200"
                     /old_locus_tag="pgaptmp_004640"
     CDS             complement(149..448)
                     /locus_tag="ACHKNP_RS23200"
                     /old_locus_tag="pgaptmp_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HGU4706664.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGLTNKKRPVCKTERSEQIQMEF"
     gene            complement(587..820)
                     /locus_tag="ACHKNP_RS23205"
                     /old_locus_tag="pgaptmp_004641"
     CDS             complement(587..820)
                     /locus_tag="ACHKNP_RS23205"
                     /old_locus_tag="pgaptmp_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4706665.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALANTSVGYLDQIVYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            934..1638
                     /locus_tag="ACHKNP_RS23210"
                     /old_locus_tag="pgaptmp_004642"
     CDS             934..1638
                     /locus_tag="ACHKNP_RS23210"
                     /old_locus_tag="pgaptmp_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4706666.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEERDGWLNIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKVF
                     PGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILKQ
                     SRHLDDIEAREWLKSS"
     gene            1907..2269
                     /locus_tag="ACHKNP_RS23215"
                     /old_locus_tag="pgaptmp_004643"
     CDS             1907..2269
                     /locus_tag="ACHKNP_RS23215"
                     /old_locus_tag="pgaptmp_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706667.1"
                     /translation="MTQNVLPINKSLHDRAVDEFNRLHGTMIGEISAMLKTAKVAPLV
                     DLRKKDPTFSNVVAELRTFRDVCNALLPYFRVDKTSEIAVIDKLLILANDLAQAIDAD
                     DPDALCAAIAALDVEPYI"
     gene            2285..2413
                     /locus_tag="ACHKNP_RS23220"
                     /old_locus_tag="pgaptmp_004644"
     CDS             2285..2413
                     /locus_tag="ACHKNP_RS23220"
                     /old_locus_tag="pgaptmp_004644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706668.1"
                     /translation="MTKAFDYTAVSKLLAEMRGCVERVQNLRRDFEAHINHSQKAA"
     gene            2679..2898
                     /locus_tag="ACHKNP_RS23225"
                     /old_locus_tag="pgaptmp_004645"
                     /pseudo
     CDS             2679..2898
                     /locus_tag="ACHKNP_RS23225"
                     /old_locus_tag="pgaptmp_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2876..3148
                     /locus_tag="ACHKNP_RS23230"
                     /old_locus_tag="pgaptmp_004646"
     CDS             2876..3148
                     /locus_tag="ACHKNP_RS23230"
                     /old_locus_tag="pgaptmp_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HGU4706669.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVAIYAAGYRKSKQLTAR"
     gene            complement(3172..3711)
                     /locus_tag="ACHKNP_RS23235"
                     /old_locus_tag="pgaptmp_004647"
     CDS             complement(3172..3711)
                     /locus_tag="ACHKNP_RS23235"
                     /old_locus_tag="pgaptmp_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="super-infection exclusion protein B"
                     /protein_id="HGU4706670.1"
                     /translation="MSNWWQELLRFFLRGLTLQQLIHMLIILIALIIITPASIKEWVD
                     IRNPEILPDHWMYYAMLLCISYVLNRGMEFIFLASSDRYKKYLSKRDEAKVIVETERL
                     FNSLSIQEKEVLAFAVMANNKIVLKHGDPVALSLMRKGLLHRSGVTYSASGKEKFVIP
                     DVWFHECYMRFAGKADELI"
     gene            3967..4935
                     /locus_tag="ACHKNP_RS23240"
                     /old_locus_tag="pgaptmp_004648"
                     /pseudo
     CDS             3967..4935
                     /locus_tag="ACHKNP_RS23240"
                     /old_locus_tag="pgaptmp_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
     gene            4960..5091
                     /locus_tag="ACHKNP_RS23245"
                     /old_locus_tag="pgaptmp_004649"
     CDS             4960..5091
                     /locus_tag="ACHKNP_RS23245"
                     /old_locus_tag="pgaptmp_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HGU4706671.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            5076..5228
                     /gene="kil"
                     /locus_tag="ACHKNP_RS23250"
                     /old_locus_tag="pgaptmp_004650"
     CDS             5076..5228
                     /gene="kil"
                     /locus_tag="ACHKNP_RS23250"
                     /old_locus_tag="pgaptmp_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGU4706672.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PCYRRK"
     gene            5225..5383
                     /locus_tag="ACHKNP_RS23255"
                     /old_locus_tag="pgaptmp_004651"
     CDS             5225..5383
                     /locus_tag="ACHKNP_RS23255"
                     /old_locus_tag="pgaptmp_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706673.1"
                     /translation="MSFTDNWSDEEFIRQMKELIGNEGDIHVTCNHSEGEQVTEAHVH
                     AESSLVSP"
     gene            5485..6090
                     /locus_tag="ACHKNP_RS23260"
                     /old_locus_tag="pgaptmp_004652"
     CDS             5485..6090
                     /locus_tag="ACHKNP_RS23260"
                     /old_locus_tag="pgaptmp_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HGU4706674.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAAMQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
BASE COUNT         1948 a         1290 c         1395 g         1507 t
ORIGIN      
        1 gcctcaagca gcatatttga tagtctggcg taaccatcat cgagatctgc cacattacgc
       61 tcctgttcgg caaagttacc tctgccgaag ttgagtattt ttgctgtatt tgtcataatg
      121 actcctgtgg attgatccag taattccctc agaattccat ctggatttgt tcagaacgct
      181 cggtcttgca caccgggcgt tttttattgg tgagtccatc aagcgcatac ttaaaagctc
      241 tgctaatcgg actgatgtct gatgccattc cgaaagcaca caagaccgaa gcaataaatc
      301 tccagtccgt tctgcttatc ttcgattcat gacagccaat catctttgcc agaccgcgct
      361 gggtaagcgt tgacaggttg ataagtaaat ctgtttctgc gcgatcaaca tcacgctgtg
      421 atagtttgct gtaacttgtt tgttccattt cttaagattt ccaatagtga atagctagtt
      481 gaaaggtatg cgtggaaacg catatggcct tagttggtca gatatcttgg aactcgcttt
      541 tcagcgacgt aggacgaatg tccgttgtta caaagagcgg ggttacttat gctgctgatg
      601 ctctacgcga tacgaacacc agattttcct ttttcacagg tttataaccc gtgaaattac
      661 gagtggcttc ttcgattgca ttcgctttat ctggggaagc tcttcgaaac ccatatacaa
      721 tctgatcaag atagccaact gaagtattcg ctaatgcggc gagtcgcttc cattcctcac
      781 tagaagcctc ttttcgccag cgtagtagtt cattactcat tagtgcctcc gtttatcaca
      841 cagagcgact ttaccatttt gataaataaa acgcaatgaa gatttatcaa aatgcatatt
      901 tatccatttg ctaaatagag ggagaatcgt gggatggaaa acaaagatat tcgcaaatcg
      961 aatctggcgt ttttgctaga tgagcataaa aaaatcgcgg gtaacactaa tgcaagcttt
     1021 gccgataagc ttggagttag cccttctcaa ctcacgcaag tctccggtga aaaaagcact
     1081 cgaaacatag gggataaact agcaagaaaa ttcgaagccg cacttgggtt acctaatggg
     1141 tggcttgatt tggtacatga tgtaacacca attgcatcat gctcagattc tttaactttt
     1201 gtcggtcagg taagaaaagg gttagtgcgc gtggttggtg aggcaattct tggtgttgat
     1261 ggtgccatcg agatgaccga agagcgcgat gggtggctca atatttatag cgatgatcca
     1321 gatgcctttg gtcttcgtgt gaaaggagac agcatgtggc ccagaataaa atcaggagaa
     1381 tatgtactca ttgagcctaa caccaaagta ttcccggggg atgaggtgtt tgtcagaact
     1441 gttgaaggac acaacatgat caaagttctt ggctatgaca gagatggaga ataccaattt
     1501 acaagcatca accaggacca caggccaata acgttgcctt atcatcaagt agcaaaggtg
     1561 gagtatgtgg ctggtattct gaagcaatct cgccatctgg atgacatcga ggcaagggag
     1621 tggctgaaaa gttcgtgact tcatcgtcac atagctggta accagtggcc tgaagagacg
     1681 tttgggtgat gtgatagaga tcattattca attcgattga gaaaaatcat cataaggttt
     1741 atatccattt attagatttt atgtgtataa atagttaaaa atgcacgatg tagagatatt
     1801 tgtgcttaac atcaattaat cattgctaaa tcgtgcttat tgttcagaaa tcgcccaagt
     1861 catattgaaa atctgttggc gaacacctat ataaggagta tagttagtga cccaaaacgt
     1921 attgccaatc aacaagagct tgcatgaccg agctgttgat gaatttaatc gtctgcatgg
     1981 aacaatgatt ggcgagatta gtgcaatgct gaaaacagct aaagtagcgc cattggtgga
     2041 tcttcgtaag aaagatccta ccttttcgaa cgttgtcgca gaattacgga cattcagaga
     2101 cgtatgtaat gctctgctcc catactttcg tgtagataag accagcgaaa tcgctgtcat
     2161 cgataagtta cttatactgg ctaatgacct ggctcaagcc atcgatgctg acgatcctga
     2221 cgcattgtgc gcagcaatag cagcccttga tgttgagcct tacatttaaa cgaggggaaa
     2281 aaaaatgact aaagcgtttg actatactgc cgttagtaag cttttggctg aaatgcgcgg
     2341 atgtgtagag cgcgttcaaa atctacgccg cgactttgaa gctcacatca atcattcaca
     2401 aaaagcggct tgatacttaa gttagatgac agaacccggc ctcagcgccg ggttttcttt
     2461 gcctcacgat ccccacacct aataacacct taaccaattg tatttattga aaaattgata
     2521 gatacaactt tctaaaccac gaaattctga tccccctcaa gtctcttcat tccttctgat
     2581 aaattcccgc aatcaaataa aaaaccataa taatcaacag gatatttgaa aacccaaaca
     2641 atttatcatt ttgctattgc catcagttta tcatttcgat aaagtcaact catcagcagg
     2701 acgctgtaag ccaaacggag cagactggca ggctctttaa acaacgtcga acactcgact
     2761 acgtggctga aaagccagat cacccaacca cataagctgt gggatgcaat gccgaagtaa
     2821 ccgtctcagg aggagcttcg agattgcatc gccaaagttt attcgggagg aatctatgtc
     2881 cagaaaaaca gaatttaaag gcaccgcagc ttctcgccgt agagctcgtc gcgcaaacct
     2941 gcaaagtcag gaggcgatta gctccgacaa gctacacagg ccaacccctt cacgagtggt
     3001 cttgcaatgc aagcgcaaac cagcaatgag agcagaagtt ataactctga caacgttgac
     3061 cagaaaatat gaaggttcaa cttgtcttcc aaacgtagcc atttacgcgg caggctaccg
     3121 gaaatcaaaa caactgacag cgaggtgacg atcccccccg ccgtagagga actaaattag
     3181 ctcatcagct ttaccagcaa agcgcatata acactcatgg aaccagacgt caggtataac
     3241 aaatttctct ttacctgacg cgctgtaagt cacacctgag cgatggagaa ggccttttct
     3301 cataagagat aaagcgaccg gatcgccgtg cttaagtacg attttgttat ttgccataac
     3361 agcaaatgct aaaacctctt tttcctgaat actcagagaa ttgaataggc gctcagtctc
     3421 cacaattact ttagcctcat ctctcttact gagatatttt ttatatctgt ctgaagatgc
     3481 cagaaatata aactccatcc ccctgttcag aacataactg atacacaaaa gcattgcgta
     3541 atacatccag tgatctggaa gtatttcggg gttccttata tctacccact ctttaattga
     3601 tgctggggtg attattatca aggcaattaa aataataagc atatgaatta actgttgtag
     3661 cgtaagacca cgcaggaaga aacgtaatag ttcctgccac cagttactca tcggtgaatc
     3721 tccatcattc tctctgtagg ggtgaataga gtttatccga tttctcgctg taggggtaca
     3781 cgagaaccac cgagcctgat gtggttaaaa gacaggcata cgaataaaca ctgcactgtg
     3841 tattcattcc aacgagtgaa tacacggagc aatgtcgctc gtaactaaac aggagccgac
     3901 ttgttctgat tattggaaat cttctttgcc ctccagtgtg agggcaattt ttttgacgga
     3961 ggatatatga gtgaagtaac agatttagtt gttattgaaa aagcaaatgc aatgactgta
     4021 atttagtctg ccgaccagat tgaagaaatt cttcaaaagg ttgaacgtga agttatgtcc
     4081 tttgtgcctg atatcacaac ggcaaagggc agaaaggaga tcgcttatct ggcgtataaa
     4141 gttgcgcaga cgaaaacata tctcgatggt cttggcaaag accttgttgc tgaactgaag
     4201 gaaattccaa agctaattga tgctaaccgc aagacagtgc gcgatcgcct tgatgaactg
     4261 aaagccaagg cgcgccagcc tcttactgat tatgaggaag aacaggcgcg gattaaagcc
     4321 gaagaagaag ctaaggcagc agctgaagct ctcgcaaagc aaattgagtc tgaccatgaa
     4381 atagcgattt tgatggatcg cgaatttgac cgccaaagag aagaggcaag actcaaagcg
     4441 gagcaggaaa agcgagagca tgaagaacgc ttaaaaagag aagctgaaga gaaagccaga
     4501 gctgaagccg aagcaaaggc aaaagccgaa attgaagcag cagcaaggcg agaagcagaa
     4561 gctaaggccg cagaggaacg tgcagagcgt gaacgcattg aagccgagca acgagcacag
     4621 cgcgaagcaa aagaggcagc agaacgagct gaaagagaaa agcatgcggc aattgaagca
     4681 gaacgccgta aagcacagga ggaggcagaa cgaatcctgc gcgaggctga agcaaaagag
     4741 caagccagaa tagcagaaga aaaaagaatc aaggacgaag aagagcgtag agcaaaggat
     4801 aaagctcacc ggaaagaagt aaataacaaa atacttgctg accttatcaa ggttggcgca
     4861 tcagaagatg ttgctaaaaa tatcataaca gccatcgtaa aaggcgaatt attcgcaaca
     4921 aaaataacct actaataaaa ccaacataag gaaccaccca tgatttacgc aatcgcggga
     4981 ggcgctcgca tgggtgcctt ccaattaaat gaatctttac ttgaacgaat cacccgtaaa
     5041 ttacgtgacg gatggaaaag agttgaggtc ttattatgcg caatgaaata gccatcaatc
     5101 accagatgct tcgtgctgca cagaacaagg cagtaatagc ccgatttatt ggtgattcaa
     5161 aaatgtggct tgaagcaaat aaagcgatga aatcagctat caaccttccg tgttatcgca
     5221 ggaaatgagt tttacagata actggtcaga cgaagaattc attcgtcaga tgaaagaatt
     5281 aatcggtaac gaaggagata ttcatgtcac ttgcaaccac agtgaaggag agcaagttac
     5341 agaggcgcat gtacacgcag aaagctctct ggtatcgcca taatggtgac cgcgaaggaa
     5401 tgcgggtatg ccttaatttg tcccgagtcg aagtattaaa tcagcgttat ttccttgggc
     5461 cgtgtccatt ctgagaacaa tcatatgagc aaagaatttt acgcaagact ggcagctatt
     5521 caggagaatc tgaacgcgcc aaagaatcag tacaactcat tcggcaaata taaatacaga
     5581 agctgcgaag atattcttga aggcgttaag ccgttactga atggcctgtt tttatcaatc
     5641 agcgatgaag ttgtgttgat tggtgatcgg tattatgtga aagccacggc aactattacc
     5701 gatggcgaaa acagtcatac ggcaaccgct cttgcacgag aggaagaaag caagaaagga
     5761 atggattctg cacaagttac gggagctaca agctcttatg cacgcaagta ttgcctcaat
     5821 ggtttattcg gcattgatga tgcgaaagat gcagataccg acgagcataa acatcagcag
     5881 aacgcagcag caaagcaatc aaaaccatca cctacacctg aacaggttct aaaagcattc
     5941 actgacgcag caatgcagaa aaacaccgta gaagagctta aacaggcgtt cgccaaagcg
     6001 tggaagatgc tcgaaggcac accggagcag cacaaagcgc aggacgttta caacatcaga
     6061 cgagacgaat tagaaggagc tgctgcttaa tggcacattc gattactgta agactaaaca
     6121 agcccgcaag agagtttcag
//
LOCUS       DAZFWL020000208         6138 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFWL020000208 DAZFWL020000000
VERSION     DAZFWL020000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.209"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..934)
                     /locus_tag="ACHKNP_RS23265"
                     /old_locus_tag="pgaptmp_004653"
     CDS             complement(<1..934)
                     /locus_tag="ACHKNP_RS23265"
                     /old_locus_tag="pgaptmp_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HGU4706675.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFSYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYA"
     gene            1155..1583
                     /gene="uspG"
                     /locus_tag="ACHKNP_RS23270"
                     /old_locus_tag="pgaptmp_004654"
     CDS             1155..1583
                     /gene="uspG"
                     /locus_tag="ACHKNP_RS23270"
                     /old_locus_tag="pgaptmp_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HGU4706676.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1704..3269)
                     /gene="ahpF"
                     /locus_tag="ACHKNP_RS23275"
                     /old_locus_tag="pgaptmp_004655"
     CDS             complement(1704..3269)
                     /gene="ahpF"
                     /locus_tag="ACHKNP_RS23275"
                     /old_locus_tag="pgaptmp_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HGU4706677.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3440..4003)
                     /gene="ahpC"
                     /locus_tag="ACHKNP_RS23280"
                     /old_locus_tag="pgaptmp_004656"
     CDS             complement(3440..4003)
                     /gene="ahpC"
                     /locus_tag="ACHKNP_RS23280"
                     /old_locus_tag="pgaptmp_004656"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HGU4706678.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            4375..5121
                     /gene="dsbG"
                     /locus_tag="ACHKNP_RS23285"
                     /old_locus_tag="pgaptmp_004657"
     CDS             4375..5121
                     /gene="dsbG"
                     /locus_tag="ACHKNP_RS23285"
                     /old_locus_tag="pgaptmp_004657"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HGU4706679.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            5330..>6138
                     /locus_tag="ACHKNP_RS23290"
                     /old_locus_tag="pgaptmp_004658"
     CDS             5330..>6138
                     /locus_tag="ACHKNP_RS23290"
                     /old_locus_tag="pgaptmp_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4706680.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHP"
BASE COUNT         1617 a         1510 c         1425 g         1586 t
ORIGIN      
        1 cagcgtagac gcccggtacg ctaacaatgc cgccacgcct gaccgccgca atacactggc
       61 gcaacgcttt accgctgctg ccctccagtt tcaggttagt cagcaccgtt tccgtggtgc
      121 tgcctttcgc ttcaaaaccg acggcgtcta ttactgcatc cacgccccgg tgacctgccg
      181 tttgttcaat aattgactgt gccggatcgc tatcttcatc gaaattaatc gggatcgcgc
      241 cgtagcggtc agcggcgaaa cgcaagcggt agggatgatg atcaacaaca aaaatctgtt
      301 ccgcaccgag caaccgtgca caggcgattg tcaacaatcc cactggaccg gcaccataga
      361 ctgcaacgct tgaaccttgt tggatctgcg cattttttgc tgcctgccat gccgttggca
      421 gaatatcaga aaggaaaagg gctttatcat ctgaaagcaa aggcggtact ttaaacggcc
      481 ccacattccc tttagggacg cggacatatt ccgcctgtcc accaggaacg ccaccataca
      541 ggtgactata actaaacaat gccgcgggag ctggtatctg ttttttattg agcgcagcgc
      601 ctttacccgc attggtattt tcgcaggcgg catattgctg cattcgacag aaaaaacagt
      661 cgccacaagc aatgacaaac ggaattacca cccggtcgcc tttttgcaaa ttttttacgt
      721 cctttccggt ttcaacgact tcccccataa attcatgacc aaaaatatcg ccatgtttaa
      781 cctggggtat tttgcctcga taaagatgga ggtcagagcc acagatcgcc gttgccgtaa
      841 tacgcagaat aatatcatct gcctgttcaa tgcccggatc gggaacattt tctacctgaa
      901 cgtgatgtgg gccgtgataa gtcaatgctt tcatgcaacc tccagagaca gttcaaaaaa
      961 gagtcagtct ttaagggtag ctatcacagc gggaaaacgg gcggtaccgc aggttttggg
     1021 agaattcctg gtgttcagtg agaatatatt aatcacagaa accgttcgta aggaaaaaga
     1081 accagacaaa tatcagggat aaatattata atcatggcga atgatttgtt catgattaac
     1141 agggagaaag ggttatgtat aagacaatca ttatgccagt tgatgtattt gaaatggaat
     1201 tgagcgacaa agctgttcgc cacgctgaat tcctcgccca ggatgacgga gttattcatc
     1261 tacttcacgt actacccggg tcagccagcc tgagcctgca ccgttttgcc gctgatgtgc
     1321 gtcgttttga agagcatctg caacatgaag cagaagaacg tctgcaaacg atggtcagcc
     1381 acttcaccat cgatccttcc cgcattaaac aacatgtccg ttttggtagc gtgcgggatg
     1441 aagtcaatga gttggcagaa gaactggggg ctgatgttgt agttattggt tctcgcaacc
     1501 catcgatttc gacccatctg ttaggttcta acgcctcgag cgtgatccgc catgccaatc
     1561 tgccggtgct ggttgtgcgt taattgccag aataaatatc ccgccctgcc cggagccatc
     1621 ccggcagggc tttttaatgc cataaaaaag ccgccaggtt tgaccccggc ggctaagcaa
     1681 ttgcaggtga atcttacttc ttcttatgca gttttggtgc gaatcaggta gtcaaaagca
     1741 ctcagagagg ctttggcacc ttcgcctgtg gcgatgataa tctgcttgta cggaaccgtc
     1801 gtacagtcac ccgctgcgaa cacgcctttg acgttggttt cgcatttcgc atcaatgata
     1861 atctcaccca tgcggttacg ttcgactgcg ccttccagcc agttggtgtt cggcagcaga
     1921 ccaatctgga cgaaaatacc ggccagttcg atgttgtgaa tatcgccgct gacacgatct
     1981 cgatattcca gaccaacgac tttgctgccg tcgcctttca cttccgtggt ttgcgcattc
     2041 agaataatgt cgacgttttt caggctgcgc agtttgtcct gcagaacctg gtcggctttc
     2101 atttctggcg caaattcgag cagcgttacg tgctcaacga tacccgccag gtcaattgcc
     2161 gcttccacgc cggagttacc gccgccgata accgctacgc gtttgccttt aaacagcggg
     2221 ccgtcgcagt gcgggcagta ggtcacgcct ttggtgcgat actgatcttc gccaggaacg
     2281 ttcatgttgc gccattttgc accggtcgcc acgataatgc tgcgtgcttt cagtaccgcg
     2341 ccagaagctg tttcaatctg atgcagacca ccttcaactg ctgctgggat cagtttgctg
     2401 gcgctctggc tgtcgatcac atcaacgtcg tattcatcaa cgtgaacttt cagtgcgcct
     2461 gccagcttct gcccttcagt cttcggtaca gaaatgtagt tttcgatatc aacggtatcg
     2521 aggatctgac caccaaaacg ttcgcccatc agaccggtac ggatgccttt acgtgcggag
     2581 taaattgctg cagctgcacc cgccgggccg gaaccaacga ttaatacgtc ataagcatca
     2641 cgcttgttca gctcttctgc cgcacgtttt tccgcgcccg tatcaatttt ggcaacgatt
     2701 tcagtcaacg tcatgcggcc ctgaccaaac tctttcccgt ttacgaacac tgccggaacg
     2761 cccatcacgt tgcgatcggt gatttcgttc tggaaggtgc cgccgtcaat tgcagtgtgc
     2821 ttgatgcgcg ggttcagtac gctcatcagg ttcagcgcct gcaccacgtc cgggcagtta
     2881 tggcaagaga gcgagtaata ggtttcgaat tcaaaatcac cgtcaatatg gcgaatctgc
     2941 tccagcagag actgcgcttc tttcgacgga tgaccaccgg tccatagcaa cgccagtacc
     3001 agcgaggtga actcgtggcc cagcggggag cctgcaaaac gtggcccctg gttggaacct
     3061 gggttggtga tcaggaaaga cggcttacgc accggcaagc tgttatcttc tttaaaggtg
     3121 actttgtctg acagttctgc gatttcagcc aacagttcct tgatttctgc cgatttagcg
     3181 ctgtcatcca gcgtggcaat taactcaaca ggcttggtca atttctcaag gtaagccttg
     3241 agttgagttt tcatatttgt gtcgagcatg tttatctcct gggctttaaa catcatcatg
     3301 caagcggcct ctgcggctac ctggatgcag cttgcatcat ggtgcgggga agaaaatcgg
     3361 gtgccgcagc acccgaagaa ttaggtgaat ttccgaagta tttcgcgccg gagttggtag
     3421 cgcgaaagac gaaggaaatt tagattttac caaccaggtc cagagacgga gccagagttg
     3481 cttcaccttc tttccattta gccgggcaaa cttcacctgg gtgagaagct acgtactgtg
     3541 ctgctttgat tttacgcagc aggtcagacg cgtcacggcc aatgccttca gcggtaactt
     3601 cgattgcctg gatgataccc tgcgggtcaa caacgaaggt cgcacggtca gccagacctt
     3661 catcttcacg catgttgtcg aagttacggg tcagggcgcc agtcgggtcg ccgatcatcg
     3721 catatttgat tttagcgatg gtttcagagc tgctgtgcca tgctttgtgg gtgaagtgag
     3781 tatcggtaga tactgcgtat acgtctacgc ccagtttctg cagttcttcg tagtggtcag
     3841 caacgtcacc cagttcggtc gggcatacga aagtaaagtc agccgggtag aagaagaaga
     3901 cgctccagcg gccttcggta tctttttcgg tgatttcgat gaattcgccg tttttgaatg
     3961 cctggttttt aaaaggttta attttggtgt taatcaagga catctatact tcctccgtgt
     4021 tttcgatgag atgtaaggta accaattttt gccgattcgg ctaatgcgtt tccattatca
     4081 aatcgataag ttttacctta caaccttcgt aagacaactt tgtgaacttt gcaccgccag
     4141 cggcgataaa aagtaaaatg gccccctttc caggtgaccg tgtaatctga actacccttt
     4201 ggtatcttca gtgcctgcct gcaggctgag gtgttgccct tacaaatgca acaacgacat
     4261 ggattacaac accctcataa acaaagggca atcacctgat ctaagctctt acctatgaca
     4321 gtgataggtt atgcctttta ctcgactttt gcactgactg aaaaggacaa attaatgtta
     4381 aaaaagatac ttttactggc tctgcttcct gcaatcgcct tcgcagagga acttcctgct
     4441 ccagtaaaag cgattgaaaa acagggcatt acaatcatca aaacattcga tgcccccgga
     4501 ggaatgaaag gttatctcgg aaagtatcag gatatgggcg tcaccatcta cctgactcca
     4561 gatggtaagc acgctatctc tggttacatg tacaacgaga aaggtgaaaa cctgagtaac
     4621 acacttatcg aaaaagaaat ttacgcacca gccggacgcg aaatgtggca acggatggaa
     4681 caatcccact ggctcctcga cggtaaaaaa gatgcgccgg tcattgtcta cgtgttcgcc
     4741 gatccgttct gcccatattg taaacagttc tggcagcagg cgcgcccgtg ggtagattct
     4801 ggcaaagtgc aattaagaac attgttggtt ggggttatca agccagaaag cccggcgaca
     4861 gcagcggcaa ttcttgcctc caaagatccc gcaaaaacct ggcaagaata tgaatcctct
     4921 ggtggcaagc ttaagctaaa cgtgcctgca aacgtaagta cagaacaaat gaaagtgtta
     4981 agcgccaacg agaaactcat ggacgatctg ggggcaaatg tcacaccagc gatctattac
     5041 atgagtaagg aaaatacgct gcaacaggcc gtggggttgc ccgatcagaa aacgcttaat
     5101 atcattatgg ggaataaata aaagataatt catggatggt tgaaatacat ccctgctttt
     5161 caaccatcct aaaaaagcat gaattgctcg ctctgcccta agtggctatg ctattttaga
     5221 aaaacttaat cctgagttaa gcaaaaaaat ataatcaata aaaatatata cgcaaaaaac
     5281 attgattaag tgaatatacc atggaagaaa aatataaccg gagtagtgta tggccaatct
     5341 ctacgacttg aaaaagttcg acttaaacct tcttgtcata tttgagtgta tttaccagca
     5401 tttaagcatt agcaaagctg ctgaatcact atatatcacc ccatcagccg tgagccagtc
     5461 gttacaacgc ttacgtgcgc aatttaatga ccctttattc attcgctcag gaaaaggtat
     5521 cgcgccgacg acaacagggt tgaatctgca tcaccatctt gagaaaaacc tcaaaggcct
     5581 tgaacaaaca attaatatag tcaataagtc cgaactaaag aaaaacttta ttatttacgg
     5641 ccctcagctc atttcttgct ctaataatag tatgctgatc cgctgcctac ggcaagatgc
     5701 ctctgtcgag attgagtgcc atgatatcct catgtcagca gagaatgctg aagaactgtt
     5761 ggttcaccgc aaagccgatc tggtgatcac ccaaatgcca gtgatcagcc gttccgtcat
     5821 ttgcatgcca ttgcatacaa ttcgcaatac tctaatttgt agtaataagc atccgcgaat
     5881 tactgacaat agcacttatg aacaaattat ggcagaagag ttcacccaat taatatccaa
     5941 atcagcagga gttgatgata ttcaaatgga aatagacgag aagtttatga atcgcaaaat
     6001 ttcatttcgt ggttcctctt tgctgacaat cattaatagc attgcagtta cagatttact
     6061 gggaatagtc ccctatgagc tttataattc ctaccgtgac tttcttaatt taaaagagat
     6121 aaaactagaa catccgtt
//
LOCUS       DAZFWL020000209         6097 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFWL020000209 DAZFWL020000000
VERSION     DAZFWL020000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.210"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..1222)
                     /locus_tag="ACHKNP_RS23295"
                     /old_locus_tag="pgaptmp_004659"
                     /pseudo
     CDS             complement(1..1222)
                     /locus_tag="ACHKNP_RS23295"
                     /old_locus_tag="pgaptmp_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            1205..1903
                     /gene="tagH"
                     /locus_tag="ACHKNP_RS23300"
                     /old_locus_tag="pgaptmp_004660"
     CDS             1205..1903
                     /gene="tagH"
                     /locus_tag="ACHKNP_RS23300"
                     /old_locus_tag="pgaptmp_004660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HGU4706681.1"
                     /translation="MSDPASNGGINTPFMDLPPIYASPGDRNDDVSAAEMAQRHLAVT
                     PLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLDLQQQRNSLSDKHLRPLEDNP
                     LRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVRHHEEANRAAIVESLRVLLDA
                     FSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEELASDRQQGFEMLFNEVYAQV
                     YDRVLREKQREPEA"
     gene            1900..2424
                     /gene="tssJ"
                     /locus_tag="ACHKNP_RS23305"
                     /old_locus_tag="pgaptmp_004661"
     CDS             1900..2424
                     /gene="tssJ"
                     /locus_tag="ACHKNP_RS23305"
                     /old_locus_tag="pgaptmp_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HGU4706682.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKKYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            2427..3757
                     /gene="tssK"
                     /locus_tag="ACHKNP_RS23310"
                     /old_locus_tag="pgaptmp_004662"
                     /pseudo
     CDS             2427..3757
                     /gene="tssK"
                     /locus_tag="ACHKNP_RS23310"
                     /old_locus_tag="pgaptmp_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            3762..4523
                     /gene="icmH"
                     /locus_tag="ACHKNP_RS23315"
                     /old_locus_tag="pgaptmp_004663"
     CDS             3762..4523
                     /gene="icmH"
                     /locus_tag="ACHKNP_RS23315"
                     /old_locus_tag="pgaptmp_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HGU4706683.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            4532..>6097
                     /locus_tag="ACHKNP_RS23320"
                     /old_locus_tag="pgaptmp_004664"
     CDS             4532..>6097
                     /locus_tag="ACHKNP_RS23320"
                     /old_locus_tag="pgaptmp_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4706684.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGHVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQ"
BASE COUNT         1288 a         1778 c         1736 g         1295 t
ORIGIN      
        1 ccggtgcgag ggtgcgcacg ctctccagcc tgcccgtggc actgtagccg tattcccgcg
       61 tctgtcgcgg gccgctgatg cgcaccaggt cgccgttgtc attccacccg tagtcacggt
      121 catacaccag gctgttcagg tgctggctct gtaactggcc tgcgggggtg tatgtgctgg
      181 tcagttcata tgcggcatta ctgcctgccc tgctgccgaa gctgcgcacc gtctcacggt
      241 gcagcctgtc ccgcgtatac tcgaccagcg gcgtcccgcc cagcttcatg cccgccaggt
      301 aaccgctgcc gtaggtcagc cactccaccg gcggcaggct gtccggcgtg acgcggtttg
      361 ccagcccctg ttcgttgtag gcgtgtttcg tctcatgctg ccacagcagt tcccccgtct
      421 ccgggttctc caccgtctgc cgttcgcccg tcaggcggcc cttatcgtcg tacccgtagt
      481 ggaccgacac gcggtggcct tcactggtat ggctgagggt ggttaaccac ccgtgctcat
      541 catactgcca ctgctctgcc gggtcgccgt tcaccgtccg gtgcgtgatg cggtccgacg
      601 catcgtagtg ccagagggtg ataagcccct cgtcttcact ctgtgtgagt tttctggtca
      661 ggtcatagtg gtaacgttgc gtccggccgt caaaaccgcg ctgttcagtc agcctgtcaa
      721 ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcacg gtgatgcgcc
      781 cggcagcgtc ataccccatg ctgcgcgtca gaccgccttc cgtcacactc accggacgcc
      841 cgcgtttatc atatgcgatg gtgctgcgtt taccgtccgg tgataccgtt gctgtcaggt
      901 ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccttc tgactgacca
      961 gttgcccacg cgggttataa ctgctgtaag tgctgatgcc ttcctcccgg tgaacggcga
     1021 tttgttgacc gtaccggtcg tactcatacc gcgtcgtgta ccccgagcag tccgtaaagg
     1081 ccagcagttg accgtaacgg ctccatgcca tctgttttgt actgcccgtc gcgtcctgta
     1141 tccctgtcgg cagctcactc gccggccgct ttcaggcatt gcggctgtcc ggccatcggc
     1201 accgctgtcc gatccggcaa gtaatggggg gatcaatact ccgtttatgg atctgccgcc
     1261 catttatgcc agccccggcg atcgtaatga cgacgtctct gcggcagaaa tggcgcaacg
     1321 ccaccttgcg gtcaccccct tactgcgcgg tcttggcggc tcgcttacca tgagcaattc
     1381 cgacgatgcg gatgattttc tggaggaggc cggacgaacg ttacaggccg caataaaagg
     1441 tctgctcgat ttgcagcagc agcgtaacag cctctcagac aaacatttgc gcccgctgga
     1501 agataacccg ctgcgcctga acatggatta cgccaccgcg ctcgacgtaa tgtttgccga
     1561 aggtaaaagc ccggtacatc tggcggctcc cgccgccgtc agtgaaagcc tgcgcaatgt
     1621 ccgccaccac gaagaagcta accgggcagc gattgtggag tcgcttcgtg tcctgctgga
     1681 tgctttctca ccacaaaatc tgctgcgccg ctttgtgcag taccgccgca gccatgaact
     1741 gcgccagccg ctggatgatg ccggagcatg gcaaatgtac agccattatt acgaagaact
     1801 ggcctccgat cgccagcagg ggtttgagat gctgtttaac gaggtctacg cccaagtcta
     1861 tgaccgggtg cttcgtgaaa aacagcggga gccggaagca tgaacaggaa ggcgtttctg
     1921 gcctgcgtgc tgatgtgcgt catattaacc ggctgcgaaa cggcgaaaaa aatcagtgag
     1981 gtgatcaaaa acccggatat ccaggtcggg agcctgaagg agcaaccttc ggaaataacc
     2041 gtcaccctgc tgaccgaacc ggacaccaat accaatgcgg aaggtgaatc agccgccgtg
     2101 gatgtgcaac tggtgtacct gactgacgac tcaaaattgc tggcagccga ctatgaccag
     2161 atagccagta cgccgctacc ggatgtgctg ggtaaaaaat atatcgacca ccaggatttc
     2221 aacctgttgc cggacaccat caaaaccctg ccgccggtaa agctggatga gaaaacgcag
     2281 tttattggtg tcgtggccta tttctccgat gaccaggcca ctgaatggaa acaaatcgag
     2341 acggtggaag gaaccggcca ccattaccgc ctgctggtgc atgtacgtca aagcagcatc
     2401 gaaatgaaaa aagaggatga ataattatgg caaccacccg caacaaggtg atgtggcagg
     2461 aagggatgct gatgcgtcca caccatttcc agcagcagca gcgttacaac gactacctgg
     2521 ataaccagcg tttccgggcc atgaatgatt tatcctgggg atttaccgaa ctcactctca
     2581 acaatgaact gctggcacag ggtaagatca tgattgacag cgcgtcaggc acactgcccg
     2641 acggcaccgt cttttctatc cccgaccagg acgcactgcc cgatccgctg cacccgcaac
     2701 attttccgga cgagagaagc cgcaatatct acctcgctct gccggtcgcc agtgatgtga
     2761 gaaatgaaat cagcgacggg cggcgaatcg ggcgttaccg gctgaattat gccgatgtcc
     2821 gggatttgca ttcagaagaa ggtgacacgc gaacactgac gctgggacaa ctgacgccgc
     2881 gcatatgagc ggtgcagaag atatgagcgc ctatattacg ctcccgcttt gccgtatcag
     2941 cgatcgccat gccgatggct ccctgacgtt ggatgacgat tttatcccct cctgccagaa
     3001 tattcaggtc agtaagaaac tgcgtgttta tctcaaagag gtacaggggg ccattggcgg
     3061 acgggcaagc gatctggcaa accgtattgg ctctccggcg cagagcggca tcgcggatgt
     3121 ggcggaattt atgatgttgc agttacttaa ccgtaaccag acccggttta cccatcgcgc
     3181 ccgccgatcc cagctccacc cggaagattt ctaccttgat cttgctgggt tgctgggtga
     3241 actgatgacc tttaccgagc cgtcgcgcct gccctgcccg cttgatgtgt atgatcatca
     3301 tgatctgacc aaaacattta aaacactgtt accggaagtc aaacgggcgc tgcataccgt
     3361 actgtcgcca agagcggtca atctgccgct gcatctgcgt gacggtatct ggcaggccga
     3421 tatccatgac acagaactgc tgcaatctgc cacctttgtg ctggccgtgg cggcaaacgt
     3481 gccggtcgat cagatccagc gtcagtttat ccagcagtcg aaaatttcct cgccggaaaa
     3541 aatccgcaat atggtcagtg tgcagatccc cggcattcca ttgcgcgcac tgatggtggc
     3601 tccccgccag cttccttacc attccgggtt cagctatttc gaactcgaca agagcggaca
     3661 ggcctggaca gaaatggctg ccgccggggc cgttgcactg catgtatccg gcagtttccc
     3721 ggatctgaac atgcaactgt gggcgataag agggtaagac gatggatgag gggtcactgt
     3781 ctctgccacc atttaccgga tacgacgaaa aaagccagcg cgactatcat ctcgcgcttc
     3841 gtggcaacag cctgaatccg atgatcgacg cagccacgcc gctcctcggt atggtgatgc
     3901 gcctttcaac catgaacagc cagacgatgc cggagcatct ttttgctcag gtggtgacgg
     3961 atgttcaggc cgtggagcaa ctgcttcagg agcagggcta cgaaccgggc gtgattatct
     4021 cgtttcgcta catcctctgc acgtttattg atgaagcggc gctcggtaac ggctggtcga
     4081 acaagaacga gtggatcaag cagtcattgc tggtccattt ccacaacgaa gcctggggtg
     4141 gagagaaagt tttcatcctg ctggagcgcc tgatccgtga acccaaacgt taccaggatc
     4201 tgctggaatt tctctggctc tgtttttctc tgggatttcg cgggcgctac aaagtggcag
     4261 cacaggatca gggggaattt gagcagatct accgccgcct gtaccacgtc ctgcataaac
     4321 tacgcggcga tgcccctttc ccgctgttac accaggacaa aaaaacccag ggcgggcgtt
     4381 atcagctcat cagtcgcctg accattaaac atattttttg tggtggcgtt gtcgtgctgg
     4441 cgttgtttta cctgttctat ctgttgcggc tggacagcca gacgcaggac atcctgcacc
     4501 agctaaacaa attacttcga taaggacgtt tatgatccag attgatcttc ccacgctggt
     4561 aaaacggctt aacctgttct cccgccaggc gctggagatg gccgcctctg aatgtatgag
     4621 ccagcaggca gcggaaatta ccgtcagcca tgtgcttatt cagatgctcg ccatgccacg
     4681 cagtgacctg cgggttatta cccgccaggg cgatattggc atggaagagt tgcgccaggc
     4741 gctgacggtg gagaactaca caactgcccg ttctgcggac agctacccgg cattttcccc
     4801 gatgctggtt gagtggctga aagagggctg gctgctggcg tcggctgaga tgcagcacag
     4861 cgaattgcgt ggcggcgtgt tgctgctggc cctgctgcat tcgccgctgc gttatatacc
     4921 gcctgctgcc gcccggctgt tgaccggcat taaccgtgac cgtctgcaac aggactttgt
     4981 gcaatggaca caggagtcgg cggaatcagt cgtgccggat gcagacggta aaggcgcagg
     5041 cacactgacg gacgctgctg acaccctgct tgcccgctac gccaaaaaca tgaccgcaga
     5101 cgcccgtaac ggcaggcttg acccggtact gtgccgcgac cacgaaatcg acctgatgat
     5161 cgacattctc tgccgccgcc gcaagaacaa cccggtggtg gtgggcgaag caggcgtggg
     5221 taaaagcgcc ttaatcgaag ggctggcgct gcgcatcgtg gcaggccatg tgccggacaa
     5281 gctgaaaaac accgatatca tgacccttga cttgggcgca ttgcaggccg gagcgtcggt
     5341 gaagggtgaa ttcgaaaaac gtttcaaagg gctgatggca gaggtcattt cctccccggt
     5401 gccggtcatt ctgtttatcg acgaagcaca taccctgatt ggcgcgggca accagcaggg
     5461 cgggctggat atctccaacc tgctcaaacc ggcgctggcg cgcggcgagc tgaaaaccat
     5521 cgccgccacc acctggagcg agtacaaaaa atacttcgaa aaagatgccg ccctgtcgcg
     5581 ccgcttccag ttggtgaagg tcagcgaacc caacgctgcc gaagccacca ttattctgcg
     5641 cggtctgtcg gcggtctatg aacagtctca cggcgtgctg attgatgatg acgccttgca
     5701 ggccgctgcg acattaagcg agcgttatct ctccgggcgt cagttaccgg acaaagcgat
     5761 tgatgtgctg gataccgcct gcgcccgtgt ggccatcaac ctgtcgtcgc cgccgaagca
     5821 aatctcggcg ctgaccactc tgagccacca gcaggaggcg gaaattcgcc agcttgagcg
     5881 cgagcttcgc atcggactgc gtaccgacac atcacggatg accgaggtgc tggtgcagta
     5941 tgatgaaacg ctgacggcgc tggatgaact ggaagcggcc tggcaccagc agcagacgct
     6001 ggtccgggag attattgcgc tgcgccagca gttactgggc gtggcagagg acgatgcggc
     6061 gccgttgccg gacgcagata ccgtggagga tacgcag
//
LOCUS       DAZFWL020000210         5998 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFWL020000210 DAZFWL020000000
VERSION     DAZFWL020000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.211"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(371..847)
                     /locus_tag="ACHKNP_RS23325"
                     /old_locus_tag="pgaptmp_004665"
     CDS             complement(371..847)
                     /locus_tag="ACHKNP_RS23325"
                     /old_locus_tag="pgaptmp_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HGU4706685.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(906..2195)
                     /gene="bioA"
                     /locus_tag="ACHKNP_RS23330"
                     /old_locus_tag="pgaptmp_004666"
     CDS             complement(906..2195)
                     /gene="bioA"
                     /locus_tag="ACHKNP_RS23330"
                     /old_locus_tag="pgaptmp_004666"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HGU4706686.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2282..3322
                     /gene="bioB"
                     /locus_tag="ACHKNP_RS23335"
                     /old_locus_tag="pgaptmp_004667"
     CDS             2282..3322
                     /gene="bioB"
                     /locus_tag="ACHKNP_RS23335"
                     /old_locus_tag="pgaptmp_004667"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HGU4706687.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3319..4473
                     /gene="bioF"
                     /locus_tag="ACHKNP_RS23340"
                     /old_locus_tag="pgaptmp_004668"
     CDS             3319..4473
                     /gene="bioF"
                     /locus_tag="ACHKNP_RS23340"
                     /old_locus_tag="pgaptmp_004668"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HGU4706688.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4460..5215
                     /gene="bioC"
                     /locus_tag="ACHKNP_RS23345"
                     /old_locus_tag="pgaptmp_004669"
     CDS             4460..5215
                     /gene="bioC"
                     /locus_tag="ACHKNP_RS23345"
                     /old_locus_tag="pgaptmp_004669"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HGU4706689.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            5208..5885
                     /gene="bioD"
                     /locus_tag="ACHKNP_RS23350"
                     /old_locus_tag="pgaptmp_004670"
     CDS             5208..5885
                     /gene="bioD"
                     /locus_tag="ACHKNP_RS23350"
                     /old_locus_tag="pgaptmp_004670"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU4706690.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         1423 a         1575 c         1663 g         1337 t
ORIGIN      
        1 tttaggcata gccataatgc atgatggtgt ggcgtggcaa agcagagata tacaaagtct
       61 gccacatgtt tttatgacaa aaaattagcg caagaagaca taaaatcacc ttgcgctaat
      121 gctctgtctc aggtcactaa tactatctaa gtagttgatt catagttact agatatgttg
      181 tgttttgtag tattatgcag tctatttttt agactaaata tattgtaaca cattgatatt
      241 aatggtcttt agcgtttcgc gttcagcttt tttatactaa cttgagcgaa acgggaaggt
      301 aaaaagacaa aaagttgttt ttaatacctt taagtgatac cagatggcat tgcgccatct
      361 ggcagagtga ttaactaaac atcgcagtaa tcgaggcgct tgccagagag tggaaatgaa
      421 cgttaaaccc gaccatcgcg ccgctggcac cttcatcgac atcaatacgt tctatatcca
      481 gcgcgtgaac ggtaaaaatg tagcgatgag tttcgccttt cggcggtgct gcgccatcgt
      541 acccggtttt accaaagtcg gtacgcgtct gcaaaacgcc gtctggcatt gctaccagac
      601 cagagccaaa cccttgcggt aatacgcggg tatcagcggg taagttaaca actacccagt
      661 gccaccagcc ggagccggtt ggcgcatccg ggtcgtagca ggtgacaaca aaacttttcg
      721 ttcccgcagg aacatcatcc cacgccagat gcggtgaaat attatcgcca tcgtaaccca
      781 tgccgttaaa gacatgacga tgcggcaatt tatcgccatc gcgcagatcg ttactgatga
      841 gtttcatgaa ccctcctttc ttgtttgcag aaagtgtagc cagaaaccct cacgcggact
      901 tctcgttatt ggcaaaaaaa tgtttcatcc tgtaccgcgc ggttaaccgc tgcggtcaga
      961 cgctgcaact gttgcgggag aataatatag ggcggcatca ggtaaatcag tttgccaaaa
     1021 ggccggatcc agacaccctg ttcgacaaag aatttttgca gcgccgccat attcaccgga
     1081 tgagtggttt cgactacgcc gattgccccc agcacgcgca catcggcaac catttcggca
     1141 tcacgggcgg gggcaagttg ctcgcgcagc tgtacttcaa tagccgccac ctgttgctgc
     1201 cagtcgccag attcgagaat cgccaggctg gcgtttgctg ccgcgcaggc cagcggattg
     1261 cccataaaag ttggcccatg cataaagcaa ccggcttcgc cgttactgat ggtttccgca
     1321 acctcgcgcg tggtgagcgt ggcggaaagg gtcattgtgc cgccggttaa ggctttaccg
     1381 aggcacaaaa tgtccggcgc gatttctgca tgttcacagg caaacagttt gccggtacga
     1441 ccaaatccgg tggcgatctc gtcggcaatc agcaagatac cttcgcgatc gcacattttg
     1501 cggattcgtt ttaaccattc cggatggtac atgcgcatcc cgcctgcgcc ctggacaatc
     1561 ggttcaatga tcaccgccgc gatttcatga cgatgcgccg ccatcaggcg ggcaaagcca
     1621 accatatcac gttcatccca ttcaccatcc atgcggcttt gcggggcggg agcaaacaag
     1681 ttttctggca gatagccttt ccacaggctg tgcattgagt tgtccggatc gcacaccgac
     1741 atcgcgccaa aggtatcgcc atgataacca ttgcggaagg tcagaaaacg ctggcgcgct
     1801 tcgcctttgg cctgccagta ctgcaacgcc attttcatcg ccacttccac cgctacggag
     1861 ccggagtccg cgagaaaaac gcattccagc ggttgcggcg tcattgccac cagtttgcgg
     1921 catagctcga tggcgggcgc atgggtgata ccgccaaaca tcacatgcga catagcatca
     1981 atttgcgact tcatcgccgc attaagctgc gggtgattat agccgtggat cgccgcccac
     2041 caggacgaca taccgtcaac caggcgtctg ccgtcagaca aaattagctc gcaaccttcg
     2101 gcgctcgcga ccggataaac cggcagaggg gaggtcatgg atgtgtatgg gtgccagata
     2161 tggcgttggt caaaggcaag atcgtccgtt gtcataatcg acttgtaaac caaattgaaa
     2221 agatttaggt ttacaattct acaccgaatt aacaacaaaa aacacgtttt ggagaagccc
     2281 catggctcac cgcccacgct ggacattgtc gcaagtcaca gaattatttg aaaaaccgtt
     2341 gctggatctg ctgtttgaag cgcagcaggt gcatcgacag catttcgatc ctcgtcaggt
     2401 gcaggtcagc actttgctgt cgattaagac cggagcttgt ccggaagatt gcaaatactg
     2461 cccgcaaagc tcgcgctaca aaaccgggct ggaagccgag cggttgatgg aagttgaaca
     2521 ggtgctggag tcggcgcgca aagcgaaagc tgcgggatcg acgcgtttct gtatgggcgc
     2581 ggcgtggaag aatccccacg aacgcgatat gccgtacctg gaacaaatgg tgcagggggt
     2641 aaaagcgatg gggctggagg cgtgtatgac gctgggcacg ttgagtgaat ctcaggcgca
     2701 gcgccttgcg aatgccgggc tggattacta caaccacaac ctcgacacct cgccggagtt
     2761 ttacggcaat atcatcacca cccgcactta tcaggaacgc ctcgatacgc tggaaaaagt
     2821 gcgcgatgcc gggatcaaag tctgttctgg cggcattgtg ggcttaggcg aaacggtaaa
     2881 agatcgcgcc ggattattgc tgcaactggc aaacctgccg acgccgccgg aaagcgtacc
     2941 aatcaacatg ctggtgaagg tgaaaggcac gccgctggcg gataacgatg atgtcgatgc
     3001 ctttgatttt attcgcacca ttgcggtcgc gcggatcatg atgccgacct cttacgtgcg
     3061 cctttctgcc ggacgcgagc agatgaacga acagactcag gcgatgtgct ttatggcagg
     3121 cgcaaactcg attttctacg gttgcaaact gctgaccacg ccgaatccgg aagaagataa
     3181 agacctgcaa ctgttccgca aactggggct aaatccgcag caaactgccg tgctggcagg
     3241 ggataacgaa caacagcaac gtcttgaaca ggcgctgatg accccggaca ccgacgaata
     3301 ttacaacgcg gcagcattat gagctggcag gagaaaatca acgcggcgct cgatgcgcgg
     3361 cgtgctgccg atgccctgcg tcgccgttat ccagtggcgc aaggagccgg acgctggctg
     3421 gtggcggatg atcgccagta tctgaacttt tccagtaacg attatttagg tttaagccat
     3481 catccgcaaa ttatccgtgc ctggcagcag ggtgcggagc aatttggcgt cggtagcggc
     3541 ggttccggtc acgtcagcgg ttatagcgtt gcgcatcagg cgctggaaga agaactggcc
     3601 gagtggctgg gctattcgcg ggcactgcta tttatctctg gttttgccgc taatcaggcg
     3661 gtcattgccg cgatgatggc aaaagaggac cgcattgtcg ctgaccggct tagccatgcc
     3721 tcattgctgg aggctgccag tttaagcccg tcgcagcttc gccgttttgt tcataacgat
     3781 gtcactcatc tggcgcgact gcttgcttcc ccctgtccgg ggcagcaact ggtggtgaca
     3841 gaaggcgtgt tcagcatgga cggcgatatt gcgccactgg cggaaatcca gcaggtaacg
     3901 caacagcaca atggctggtt gatggtcgat gatgcccacg gcacgggcgt tatcggggag
     3961 caggggcgcg gcagctgctg gctgcaaaag gtaaaaccag aattgctggt ggtgactttt
     4021 ggcaaaggat ttggcgtcag tggggctgcg gtgctttgct ccagtacggt ggcggattat
     4081 ctgctgcaat tcgcccgcca ccttatctac agcaccagta tgccgcccgc tcaggcgcag
     4141 gcattacgtg cgtcactggc ggtcattcgc cgtgatgagg gggatgcacg gcgcgaaaaa
     4201 ctggtgtcac tcattgcgcg ttttcgtgcc ggagtacagg atttgccgtt tacgctggct
     4261 gattcatgca gcgccatcca gccattgatc gtcggtgata acagccgtgc gttacaactg
     4321 gcagaaaaac tgcgccagca aggctgctgg gtcacggcga ttcgcccgcc aaccgtaccc
     4381 gctggtactg cgcgactgcg cttaacgcta accgcagcgc atgaaatgca ggatatcgac
     4441 cgtctgctgg aggtgctgca tggcaacggt taataaacaa gccattgcag cggcatttgg
     4501 tcgggcagcc gcactctatg agcaacacgc agatctacag cgccagagtg ctgacgtctt
     4561 actggcaatg ctcccacagc gtaaatacac ccacgtactg gacgcgggtt gtggacctgg
     4621 ctggatgagc cgccactggc gggaacgtca cgcgcaggtg acggccttag atctctcgcc
     4681 gccaatgctt gttcaggcac gccagaagga tgccgcagac cattatctgg cgggagatat
     4741 cgaatccctg ccgttagcga ctgcgacgtt cgatcttgca tggagcaatc tcgcagtgca
     4801 gtggtgcggt aatttatcca cggcactccg cgagctgtat cgggtggtgc gccccggagg
     4861 cgtggtggcg tttaccacgc tggtgcaggg atcgttaccc gaactgcatc aggcgtggca
     4921 ggcggtggac gagcgtccgc atgctaatcg ctttttaccg ccagatgaaa tcgaacagtc
     4981 gctgaacggc gtgcattatc aacatcatat tcagcccatc acactatggt ttgatgatgc
     5041 gctcagtgcc atgcgttcgc tgaaaggcat cggtgctacg catcttcatg aagggcgcga
     5101 cccgcgaata ttaacgcgtt cgcaattgca gcgattgcaa ctggcctggc cgcaacaaca
     5161 ggggcgatat cctctgacgt atcatctttt tttgggagtg attgctcgtg agtaaacgtt
     5221 attttgtcac cggaacggat accgaagtgg ggaaaaccgt cgccagttgt gcacttttac
     5281 aagccgcgaa ggcagtaggt tatcggacgg caggttataa accggtcgcc tctggcagcg
     5341 aaaagacccc ggaaggctta cgtaatagtg acgcgctggc gttacagcac aacagcagcc
     5401 tgcagctgga ttacgcaacg gtaaatcctt acaccttcgc agaacccacc tcgccgcaca
     5461 tcatcagcgc gcaagagggc aggccgatag aatcattggt aatgagcgcc ggattacgcg
     5521 cgcttgaaca acaggctgac tgggtgttag tggaaggtgc tggcggctgg tttacgccgc
     5581 tttctgacac tttcactttt gcagattggg taacacagga acaactgccg gtgatactgg
     5641 tagttggtgt gaaactcggc tgtattaatc acgcgatgtt gactgcgcag gcaatacaac
     5701 acgccggact gactctggcg ggttgggtgg cgaacgatgt tacgcctccg ggaaaacgtc
     5761 acgctgaata tatgaccacg ctcacccgca tgattcccgc gccgctgctg ggagagatcc
     5821 cctggcttgc agaaaatcca gaaaatgcgg caaccggaaa gtacataaac cttgccttgt
     5881 tgtagctatc ctgtatttgg ttaaattgcg agcgagatcg cgtcttcgat tgactgcaat
     5941 ttaaccaatt aaattctaaa ataatcacga aaaaaatttt acttccgcct catgcggc
//
LOCUS       DAZFWL020000211         5961 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFWL020000211 DAZFWL020000000
VERSION     DAZFWL020000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.212"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            49..288
                     /locus_tag="ACHKNP_RS23355"
                     /old_locus_tag="pgaptmp_004671"
                     /pseudo
     CDS             49..288
                     /locus_tag="ACHKNP_RS23355"
                     /old_locus_tag="pgaptmp_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHKNP_RS23360"
                     /old_locus_tag="pgaptmp_004672"
     CDS             complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHKNP_RS23360"
                     /old_locus_tag="pgaptmp_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HGU4706691.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(965..1639)
                     /locus_tag="ACHKNP_RS23365"
                     /old_locus_tag="pgaptmp_004673"
     CDS             complement(965..1639)
                     /locus_tag="ACHKNP_RS23365"
                     /old_locus_tag="pgaptmp_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HGU4706692.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHKNP_RS23370"
                     /old_locus_tag="pgaptmp_004674"
     CDS             complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHKNP_RS23370"
                     /old_locus_tag="pgaptmp_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HGU4706693.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2114..3784)
                     /gene="ureC"
                     /locus_tag="ACHKNP_RS23375"
                     /old_locus_tag="pgaptmp_004675"
     CDS             complement(2114..3784)
                     /gene="ureC"
                     /locus_tag="ACHKNP_RS23375"
                     /old_locus_tag="pgaptmp_004675"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HGU4706694.1"
                     /translation="MFGPTTGDKIRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGM
                     GQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIG
                     VSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTGPTAGSNATTCT
                     PGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKIHEDWGATPAVI
                     NCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGAGGGHAPDIITA
                     CAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRQETIAAE
                     DVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEESGDNDNVRVKRY
                     IAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKGGMIAMAPMGDI
                     NGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLHSTTAVVKGCRT
                     VQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFLF"
     gene            complement(3810..4130)
                     /locus_tag="ACHKNP_RS23380"
                     /old_locus_tag="pgaptmp_004676"
     CDS             complement(3810..4130)
                     /locus_tag="ACHKNP_RS23380"
                     /old_locus_tag="pgaptmp_004676"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HGU4706695.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVDLVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4139..4441)
                     /locus_tag="ACHKNP_RS23385"
                     /old_locus_tag="pgaptmp_004677"
     CDS             complement(4139..4441)
                     /locus_tag="ACHKNP_RS23385"
                     /old_locus_tag="pgaptmp_004677"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HGU4706696.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(4451..5230)
                     /locus_tag="ACHKNP_RS23390"
                     /old_locus_tag="pgaptmp_004678"
     CDS             complement(4451..5230)
                     /locus_tag="ACHKNP_RS23390"
                     /old_locus_tag="pgaptmp_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HGU4706697.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQQVMRDIWQFMRPQLTDKSPVLPRIWLT"
     gene            5611..5886
                     /locus_tag="ACHKNP_RS23395"
                     /old_locus_tag="pgaptmp_004679"
     CDS             5611..5886
                     /locus_tag="ACHKNP_RS23395"
                     /old_locus_tag="pgaptmp_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706698.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
BASE COUNT         1351 a         1542 c         1600 g         1468 t
ORIGIN      
        1 ctgtagattc aatctgtcaa tgcaacaccc ctttcaatta tctctttcac tgcccgcgac
       61 ggtatcggtt atcagtttga cctgctggct aacggtgaaa ccgtactgcg tgatgcatcc
      121 ggcgaaaaac gcatcaaggg tgaaaaagac ggtcgtatgc tcatgaatgt tggcctgaat
      181 gcggaaattc gcgacaacat ccgcttcggt ctggagtttg aaaaatcggc atctggtaag
      241 tacaacgtgg ataacgcggt gaacgccaac ttccgttatt ccttctgata aaaacaaggg
      301 gccgcctgcc ggccctgata cagaggatgt tgtgattact tacccagcat gccctgctct
      361 tcgataaagg cgatgatgtt ttgcagacct tcaccacttt tcaggttagt gaacccccat
      421 gggcgttctc cgcgcatacg taaagtatca cgctccatta cctcgaggga ggcgcccacg
      481 tatggtgcaa ggtcagtttt attgatcacg aggaagtcgg atttggtgat ccccggaccg
      541 cctttacgcg ggatcttttc tccttctgcc acatctatga catatatggt aaggtccgcc
      601 agttcggggc tgaaggtcgc actcaggttg tcgccgccgc tttccacaaa gatcagatcg
      661 agattaccaa atttctcact cagtgcttcc acggctgcca gattcattga tgcgtcttca
      721 cgaatggctg tatgagggca gccccccgtt tcgacaccga caatgcgttc cgcttccagt
      781 gctccggcct cagtgagaat gcgttgatct tctctggtgt agatgtcgtt ggtgactacg
      841 gctaattgcc aggtgtcacg catggatttg cagagggctt ccagaagcgc ggttttacct
      901 gagccgaccg gaccgccgac gccgacacgc agcggttgct tgattatgtt cattttattt
      961 tctcctaaga acggaataat cgggaatact gggtttcatg tcgcgcggaa gcgatagatg
     1021 caagtggcgt tgctgaacca attgcgtgat ccggggttgc aagggcactg tccatgttcg
     1081 ccgcatagcg ggcacacagg cgcataatta actgctgggc agccagctgg ccgtagggta
     1141 ccagcctgac gcccgccatc actgcgcttt caatccagct gtagccaagg ctcagggcca
     1201 ggtctgaaac ggatatttgc cagcgtacgc cgagccatgc cataccggtt aactggcttt
     1261 gctggcatag tttgcgccat tcggccgggc agtcgggctg ccagtcgacc agaagtctgg
     1321 tgaaggctgc accgcggtta cgttcttcat cgcgtagctc acgggtttcc ctgcaggcca
     1381 gcagataagc ggtccagcgc ccggcactgt caggatcgcc cgcttcacag gcccggtata
     1441 gccgggcaag cagtggcagg tcgacggcaa aaaaactctg ttccatctgc aactgctgcc
     1501 agtgttcaaa ggcggcgcta tcttctaccc agcctgcttc cactgcccac tccagtccct
     1561 gggaccagct gtagcctcca acagggaggc tgttactggc aagctgcatc agccgcagac
     1621 gtttttccgg agtcggcatt cagtggctgt gctcctgatc gtgattatgg gcgtgcgact
     1681 tgctggcgta agcgccagct tccggctcaa acggaagatg tgcaaaatca acatccagcc
     1741 caaactggcg cagcatatca tcgaggacgt gatcgtgatg gtagcgcagc tctccgggca
     1801 ttatttgcag cggaacgtgg cgattgccga gatgccagca ggctttcgcg agcataaaag
     1861 gatctgcgca gcgcactaca gaaacggctt catcagcggc gatcacctta atgaattcat
     1921 caccgttttc gttactgaga atatcaccat cgcgcagcag cagaccgcgt ggcagcaaca
     1981 gtccagcctg acgtccatcg ttaagggtga ctttaatacg gctttttacc cgcatgtcaa
     2041 cagggagggt aacgctggca gttgtctgag ccggggtctc cacccgccgg gtcaaataaa
     2101 gcatttgtgc tccttaaaac aggaaataac gttgcgccat tggcagaatg tccgccggtt
     2161 cactggttat cagttcgccg ttgatacgca cttcgtaggt ttgtgaatcc acggttatat
     2221 caggcaacag gctgttgtgg cgcatatcgg ctttttgtac tgtgcggcag ccttttacca
     2281 cagcagttgt gctgtgcagg ttaagctgtt cagcgacgcc atttgctgct gctgcctgcg
     2341 acaggaaagt cacacgacag cggtgacggg cactgcccaa tgcagcgaac attgggcgat
     2401 agtgcaccgg ctgcggtgtg gggatagagc cgttgatatc acccatcggc gccatggcta
     2461 tcattccgcc tttgacgata gtcgccggtt ttacgccaaa gaacgccggg gaccagagca
     2521 ccagatccgc cagttttccc acttcaatcg agccgacttc atgagcaata ccgtgggtta
     2581 atgccggatt aatggtgtat ttagcgatat agcgcttcac gcggacgttg tcgttatcac
     2641 cactttcttc cggtaacggg ccgcgctgaa ctttcatccg gtgtgccacc tgccaggttc
     2701 gtaacactac ttctccgacg cgtcccatgg cctgcgaatc ggacgaggtg agggagaacg
     2761 cgccaaggtc gtgcaggacg tcttccgcgg caatggtttc ctggcgaatg cgcgattcgg
     2821 caaaggctac gtcctcggcg atatccgggt caagatgatg gcaaaccatc agcatgtcca
     2881 gatgctcatc aatagtgttg acggtatagg gtagcgtcgg attggttgag gaaggcagaa
     2941 tattggggtg cgcgcaggcg gtgataatat ccggagcatg accaccacct gcaccttctg
     3001 tatggaaggt atggatagtg cgcccgccga tggcagtcag agtatcctca acgaatcctg
     3061 attcgttaag cgtatcactg tgtagcgcaa cctgaacgtc catttcgtcg gctacagtca
     3121 gtgcgcagtt gattaccgca ggtgtagctc cccagtcttc gtgaattttg aggccgataa
     3181 ccccggccgc gacctgctca cgaagcgcat ccggattgga gcaattgcct ttacccagca
     3241 acccgatatt gaccggcagg ctgtctgcag cctgcagcat ttgataaatg taccatggtc
     3301 ctggggtaca ggttgtggcg ttagaacccg ctgtcgggcc agtaccgcca ccgatcatgg
     3361 tggtaatgcc ggatgtcagc gcttcttcag cctgctgtgg gcagatccag tgaatatgcg
     3421 tatcgacgcc acctgcggta acgatcctgc cttctgcggc aataatttcc gtggatacgc
     3481 cgattgggat agtgacgttg ggttgtatat caggattacc ggctttgccg atagcaaata
     3541 tccttccatc tttgacgccg atatcggctt taacgatccc ccagtaatcg atgatcaggg
     3601 cattggtcag caccagatca gcgcagccgg cggagagcat ttgcccctgt cccataccgt
     3661 cgcggattac tttaccgccg ccgaatttga cctcttcgcc gtaggtagtt aaatcatctt
     3721 cgacctcgat ccacagctca gtgtctgcca gacgaatttt atcaccggtg gtagggccga
     3781 acatgtcagc ataggcctag cgtgaaatat tactcatcat ttgcctccag ctcacccatt
     3841 atatctccgc ggaaaccgtg cacgatacgg gcaccgctca gcgccaccag atcaacctca
     3901 cgcttttggc ctggttcgaa gcgcactgca gttccggcgg cgatattgag acgatagcct
     3961 cttgccttct ggcgatcgaa cttcagtgcc gggttaacct ccgcgaagtg ataatgggaa
     4021 ccgacctgaa ttggtcggtc tccgtgattt tcaacgataa tactgcaggt agcccggccg
     4081 atgttaagct ctatatagcc aggtttaacc ttatattctc cagggatcat ctgttacctc
     4141 agattatcgg attatggacg gtaaccagct tggagccgtc cggaaaggtg gcttcgacct
     4201 ggatatcggg gatcatttct ggtatgcctt ccatgacctg ctcgcgactc aggacatgcc
     4261 gtccttcttc catcagcgca gcgacgcttt tgccgtcgcg agcgccctcc attataaaag
     4321 cgctaatcag ggccacggat tcgggatagt taagtttcag gccgcgggcc agacgacgct
     4381 ctgccagcag tgcagcggta aacagtaata gtttgtcttt ttctcttgga gtcagttcca
     4441 tagcgttctc ttaagtcagc cagatccggg gaagtaccgg tgatttgtca gtcagttgcg
     4501 gccgcataaa ttgccagata tcacgcatta cctgctggca acgcagatta tcatcactta
     4561 aaaagcgaac tgtcagtaga ccatcggtga gcgtggctcc ggcgtagttt tccagtggtg
     4621 tgagtttttc gcgtatgtct tccagttgcg tttcattccc caggtagaac agcatcgttc
     4681 ccacccatgg tttccgggca acgcaggtaa gttctccatt ggccacgtgc agacgctcaa
     4741 tcaacagcgg agaaccgtcg acccatatct ccagacggtt agcgagtgtt ccgtggctga
     4801 aggtttctcc gatgaccgga cgtccaaggc acagaagatc ccaggccagc agtgtactgg
     4861 aggacgacag atgaaacacg gtatgcaacg tggcgtgagc gccgggaaaa aaaatcgaat
     4921 cctgtggcaa ccactccagc gttgcgttat tgcacagcgt caggttttgc tgaaggtgcg
     4981 cctgcgcgcc gctgctgcgg taaaatttac tggcaccagg catggtgatc agcgtatggc
     5041 aatcagagtc aattattgcg ttgatgatga gttcgtcacc gccgacgata ccaccgggtg
     5101 gatgaagcag acagaggtgg caggtttctt cttcgggata aaaaggacgc tgaacagtga
     5161 gcggaccgac atgatgacgg gtggtgagcg tggttttccc gtgcagacgc tgaaaacgga
     5221 gatccagcat ggcctgccag ccttttttat tttgtggtaa cgcggtactg agcaatctta
     5281 cgcccctgat aaataaagat aaaacgacag taaagagccg gatttccttg ttatataaaa
     5341 gatgatgcct ttccagtgag ttttcgtctg aataatacga atgtttgttt tcgtcatgaa
     5401 gcgttccggc tcccggggct atacatcgcc agaaaagcaa tgcatattgt cgggattatc
     5461 cagagaatca ggcagaaaga tccgacaatg ggctgtgatt atgccatctg gctcagaatg
     5521 atatcaaggt gttatgggga taacttgctg atacaatagc agtatgacag attcattaat
     5581 gcggaaactg ctcactgaaa cagagtgttc atgactgagc actggtcggg tcatgcagat
     5641 gataatttcc ccttttcttg gggaagcgac agtattttca tgcatgcctg tcttcagtca
     5701 tacggggaga gcatctgtga tacagtccat acatttgttg cggacgtcgc tcctgagtca
     5761 ctgactggtc tgcaaacagt ttatctgtac acggcagggc ttctgtgtga tatcgttttt
     5821 catatcgagg gggggcctcc agagatgaat ggaataataa tatttcctga tgatataata
     5881 ccttagagaa tttctgtgta tgctattgtg aacatatttt ttattaaaaa gatgttagtg
     5941 gtgttgtaaa ataaaagaat c
//
LOCUS       DAZFWL020000212         5899 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFWL020000212 DAZFWL020000000
VERSION     DAZFWL020000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.213"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(503..1693)
                     /locus_tag="ACHKNP_RS23400"
                     /old_locus_tag="pgaptmp_004680"
                     /pseudo
     CDS             complement(503..1693)
                     /locus_tag="ACHKNP_RS23400"
                     /old_locus_tag="pgaptmp_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131474.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1957..2157)
                     /gene="torI"
                     /locus_tag="ACHKNP_RS23405"
                     /old_locus_tag="pgaptmp_004681"
     CDS             complement(1957..2157)
                     /gene="torI"
                     /locus_tag="ACHKNP_RS23405"
                     /old_locus_tag="pgaptmp_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HGU4706699.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2215..2382)
                     /locus_tag="ACHKNP_RS23410"
                     /old_locus_tag="pgaptmp_004682"
     CDS             complement(2215..2382)
                     /locus_tag="ACHKNP_RS23410"
                     /old_locus_tag="pgaptmp_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706700.1"
                     /translation="MHFRVTGELNGEPFNRVIEAEDINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(2418..2717)
                     /locus_tag="ACHKNP_RS23415"
                     /old_locus_tag="pgaptmp_004683"
     CDS             complement(2418..2717)
                     /locus_tag="ACHKNP_RS23415"
                     /old_locus_tag="pgaptmp_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706701.1"
                     /translation="MACSTFNPLTLQKYQPDPEDLCSLCGGNHGKAAMIECKDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAIRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            complement(2875..3153)
                     /locus_tag="ACHKNP_RS23420"
                     /old_locus_tag="pgaptmp_004684"
     CDS             complement(2875..3153)
                     /locus_tag="ACHKNP_RS23420"
                     /old_locus_tag="pgaptmp_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HGU4706702.1"
                     /translation="MDSFAKYTIIDWIAFLQVLLIWFYMAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFYEAFNLNSLQPGYVIVVTTQSGMTIQIHKPKEEK"
     gene            complement(3153..3740)
                     /locus_tag="ACHKNP_RS23425"
                     /old_locus_tag="pgaptmp_004685"
     CDS             complement(3153..3740)
                     /locus_tag="ACHKNP_RS23425"
                     /old_locus_tag="pgaptmp_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4706703.1"
                     /translation="MTISTKKPLYAEVHNVPDDYEFTDEELNRIIAGDMFTPRQDAIM
                     AREIQKLRAAMLHGAEPVSQTYKSQHTQFEQVADLYEMQFDDGRTCAFHTDAQKAAQW
                     LQACDGNRVQEYVKLERLRNALSGNSPVTPDGWICCSERMPDKLIPVMVMYEDGEMWS
                     AMWNGNRWDDGTEYPDPHSVTHWREMPAAPQQEVK"
     gene            complement(3737..4354)
                     /locus_tag="ACHKNP_RS23430"
                     /old_locus_tag="pgaptmp_004686"
     CDS             complement(3737..4354)
                     /locus_tag="ACHKNP_RS23430"
                     /old_locus_tag="pgaptmp_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706704.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPIGEVSEKRLGLVMDGTVDLGGKSTYRIIKGEKAMKLLPLGTKFYTAPPAPIALEA
                     IENAIEYIRSIAFHIDEDDYHGKHIAYFMRQALAWLEGHSCSDDSQGKSDNSPLPRYQ
                     VIELTMLVKQLVSQLKKAKPDCKLPDRAMDYLLRNGLVSAEDVLR"
     gene            complement(4358..4546)
                     /locus_tag="ACHKNP_RS23435"
                     /old_locus_tag="pgaptmp_004687"
     CDS             complement(4358..4546)
                     /locus_tag="ACHKNP_RS23435"
                     /old_locus_tag="pgaptmp_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706705.1"
                     /translation="MSLTVRQLISKLRKMPPEAVVVWQGYDQSEGEYNDFVGHVADVT
                     DENAPSFDPEVRVVALRG"
     gene            complement(4548..5216)
                     /locus_tag="ACHKNP_RS23440"
                     /old_locus_tag="pgaptmp_004688"
     CDS             complement(4548..5216)
                     /locus_tag="ACHKNP_RS23440"
                     /old_locus_tag="pgaptmp_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4706706.1"
                     /translation="MSLIDYQALRAKAEKATCGVWSLEYGESRFDCDDALIHREVVGY
                     LPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDELERNQQYIKRRDQE
                     NEDIALTVGKLRVELEGKDKLIAELGKQCAEWERKALSNFEECAAMAERIEEMSKQSC
                     EARERDLFESWVMHSICISKSTLEGLRTETGYRNATLSGTDFNRMWKQWKSIRATGIR
                     IKGE"
     gene            complement(5693..5800)
                     /locus_tag="ACHKNP_RS23445"
                     /old_locus_tag="pgaptmp_004689"
                     /pseudo
     CDS             complement(5693..5800)
                     /locus_tag="ACHKNP_RS23445"
                     /old_locus_tag="pgaptmp_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5797..5899)
                     /locus_tag="ACHKNP_RS23450"
                     /old_locus_tag="pgaptmp_004690"
                     /pseudo
     CDS             complement(5797..5899)
                     /locus_tag="ACHKNP_RS23450"
                     /old_locus_tag="pgaptmp_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2737 family protein"
BASE COUNT         1448 a         1409 c         1224 g         1818 t
ORIGIN      
        1 tgtacttata tttttgtaat aaagatgaac gcgtccttta gataaactga aaaatattaa
       61 gcatgtctca tgtggttttc tgaatcaaaa ctcttttcag tctttgattt tttgttaaaa
      121 acaggtttat tgcaagggtg ttgttgtgaa atgtgttttg gtgtcgttgt gattttatgg
      181 tgataatgtg aatttttatc ctttcaagat ggctccctgg acgttaaata cagtggaaat
      241 ttatattaag atataaatat aactattgcg ttatttaaat tattgtctat tacagctcca
      301 gttaatagag cctgaagtaa tttcttagta tttcatcata agcgtttatc tatcgtctct
      361 ttctaaatta accggtcgta ttacaaggtg ctgaatctga ttaaggaatg atacttaggg
      421 gattatggct gacttaataa ttagataact gataaatgac tatcaggggg atatcgttaa
      481 gtcagccttt aaaatcaagc gattatatag ctggtgactc ttttcgaaga gcatcaaggt
      541 agtcagcgta ccattgcatc atttctcgtc ttccttcaag gtaacgagca tggttgtatg
      601 taccacgaat gctgtttttg tccgtatgtg caagttgcat ttcaatccat gcactattga
      661 atccttgctc gtgtagaaca gtactcattg tatgacgaaa tccatgcccg gtagcttttt
      721 tgtcatagcc aatcttcttc aaaaccatat tgacgcttgc atcgctgata ggcctgttaa
      781 catcatttct tccggcaaag atgtatctgt atcttccagt cagttctttg aggatttgca
      841 gagactggat tgcctgatct gacagaggga cgatatgctc cttcctcatt ttcatccggg
      901 tcggaggaac ttcccatatc ttttgggtaa aatcgatctc acaccattcc cccatacgta
      961 attctactgt tctcagccct gtgagcatca acaattttgt agcttcacgg gttacaatac
     1021 tgccggttta gttttcgagt ttgtataaaa actctggtag ttcaggagga agcaggaaag
     1081 ggtaatgtgt tcgcttatag cttttcattg ctataaccag atcagaagct ggattgtatc
     1141 gtgcccgacc tgtaacaatg gcataccgaa acacttcacc acatcgttgg cgaactttat
     1201 tagctagctc gcaggctcct cggctttcga tacgctgaag aactgcgaga agttcaagag
     1261 gttcgatttc actaatgggt ctgtttccaa tgtagggaaa cacattattt ttgaatgctc
     1321 tgtcgacata atcagcgtag ctggcagacc agcctgaaac ttttgcgttg taccactcgg
     1381 tggcgattgc ggcaaaagtt cttccttcgg aaaggttttt ctcaagattt tcagctttct
     1441 tgacttcacc gggatcttta ccatcggcat gtattttctt ggcttcatcc cgacgtttgc
     1501 gagcttcagc caatgtaata gttggatagg tgccaaatga tagtttcttt tccttccccg
     1561 catagtggta cttcattcgc cagtactttg agccgttagg attgacctga aggtagagcc
     1621 caccaccatc ggcaatctta taaattttat ctttgggttt ggctgtttca acttgtcgag
     1681 ctgtcagtgg catctggggg cctcgcaata gattgaccaa gatgtgcccc taaataggcc
     1741 cccaaatgtg acttgattta cgttcccctg aggagaatta aggcgactat ttgagtaaca
     1801 taagtaaggg tatgtgctga ttataaaggc tttttgtggg gtatggtaga tttcaggaga
     1861 ggtgtgcatg gtgtcccctg cagacatcta cttagagtgg caggggattg attagaatgg
     1921 tattttttag atgtgagaaa tattttaccc gctattttac ccattagcgc ggcttaagag
     1981 cttatttttg aattcacaat ggtcacgata taaccatctt gctcgaccgt ggataacttt
     2041 ggctttaggc aggtcgccgg acttaatccg gtcgtagatg aaggttttac caaagccagt
     2101 atcagccatg atgaatttca aatcaaccag tgaatcaggt tgtagttcgt gttgcatgag
     2161 tgctatctcc gaatagggaa tcgaacctgc aaatcaggca ataaaaaacc gccatcaggc
     2221 ggcttggtgt tctttcagtt cttcaattcg aatattggtt acgtctgcat gtgctatctg
     2281 cgcccatatc atccagtggt catagcagtc gttgatgtcc tctgcttcga taactctgtt
     2341 gaatggttct ccattcaatt cacctgtgac tcggaagtgc atttgtgatt tccccaaaag
     2401 atgcttgagt gcgcttctta ttcgattcgc acacctggta tttcgccttt tgaaatggct
     2461 aagtcataaa tttgcgcagc actataccca tctcgcatcc atgaatctaa ggcgcgaata
     2521 gcctcgctac gctttttatc ttctctttca tttttgatat caacgaggac atcaacgcaa
     2581 ttaaggcata tgtggatttt gtccttacat tcgatcatgg cagctttacc atgatttccg
     2641 ccacacagtg agcataaatc ttcagggtct ggctggtatt tctgtaacgt taaagggttg
     2701 aatgttgaac aggccataat catctccata aaacaaaact cgccgtagcg agttcagata
     2761 aaagaaatcc ccgtcagtgc gaggattgtt attgtctttt cttcgtgcat ttgtcgcact
     2821 ttcggcacca tccagatagg cacatccgtc cgcaattaac acatataggc cacatcattt
     2881 ttcctctttt ggtttatgaa tctgaatggt catgccgctt tgagtggtga ctacaatgac
     2941 ataaccaggc tgaagactgt taagattgaa tgcttcgtaa aacgaatcca aggccagcgc
     3001 ttttttattc tttcggttcc accaacgcca tcccttgcta caggctacac tgacaatcca
     3061 ctgcccactc ctgtaagcca tataaaacca gatgagcaaa acctgaagga atgctatcca
     3121 gtcaataatc gtatatttcg cgaaggagtc catcacttca cctcctgctg cggtgctgct
     3181 ggcatttcac gccagtgcgt aactgagtgc ggatccggat attcggtgcc atcatcccag
     3241 cgattgccat tccacattgc agaccacatc tcaccgtctt catacatgac cattaccgga
     3301 attaacttat ccggcattcg ctcactacag catatccaac catccggagt taccggagag
     3361 ttgcccgata gcgcattacg caatcgctcc agcttcacgt attcctgaac cctgtttccg
     3421 tcgcatgcct gaagccattg cgcagccttt tgcgcatcag tgtgaaaggc acaagtgcga
     3481 ccgtcatcaa attgcatttc gtagaggtca gcaacttgtt caaactgcgt atgttgtgac
     3541 ttgtaagttt ggcttacagg ttcggcacca tgaagcatgg cggcgcggag tttctgtatc
     3601 tcccgtgcca ttattgcgtc ctgacgagga gtgaacatat cacctgcaat aattctgttt
     3661 agttcctcgt cagtgaactc ataatcatca ggaacattgt gaacctcagc ataaagaggc
     3721 ttttttgtag aaatggtcat cgtaaaacat cctccgcact taccagtccg tttcgcaaaa
     3781 gataatccat cgccctatca ggtaatttgc aatcaggttt tgcttttttc agttggctga
     3841 ccaattgttt aaccagcatt gttaattcga tcacctggta gcgcggcaat ggtgaattat
     3901 cggatttgcc ctgactgtca tcactgcatg aatgcccttc cagccaggcc aatgcttgtc
     3961 gcatgaaata cgcaatatgt ttgccgtggt aatcgtcttc atcgatgtga aaagcgatac
     4021 tgcggatgta ttctattgca ttttcaatgg cctccaacgc tatcggcgct ggcggtgcgg
     4081 tataaaactt cgtccccagc ggcaacaact tcatcgcttt ttctccctta atgatgcgat
     4141 aagttgattt cccgccaagg tctaccgttc catccataac aaggccgagt cgcttctctg
     4201 aaacctcacc aataggctct gcttccagcg atgccagagc aattcgtgcc agttcttccg
     4261 cttcttctgc tggcagtaca acgttgctac caggtccgta tgtttcgcgc cactgcttga
     4321 ttgtcagtag tcgctctttg gtaatagtgg tcatgggtta tcctcttagc gccacaacgc
     4381 gcacttctgg gtcaaaagat ggtgcattct catctgtaac atcagcaaca tgacctacaa
     4441 agtcgttata ctcaccctct gattggtcat agccctgcca gaccacgaca gcctcaggag
     4501 gcatttttct gagcttgctg attaactgcc taaccgtcag cgacatctca ctctcctttg
     4561 atgcgaatgc cagtagcgcg gatagatttc cattgtttcc acattcggtt gaagtctgtg
     4621 cctgataagg ttgcgttacg gtatccagtt tcggtacgca atccttcaag cgtcgatttg
     4681 gagatacaaa ttgaatgcat tacccatgat tcaaacaaat cacgctcccg ggcttcgcaa
     4741 ctttgcttac tcatctcttc gatacgttca gccatcgcag cacactcttc aaagttgctt
     4801 aatgcttttc gctcccattc ggcgcattgt tttccaagct ctgcaatcag cttgtctttg
     4861 ccttccagct caacacgcag tttccctacc gttagcgcaa tatcctcgtt ctcctgatcg
     4921 cggcgtttga tgtattgctg gtttctttcc agttcatcca gtaatgccag cacagtagcc
     4981 ggattggctg cggcgatgaa ttcagcattg gcctgctgtt ccatttggaa atcttcatcg
     5041 aaaccgcttt ctggatgtgc tccttcaatt ctgcaaatgg gaagatatcc aacaacttca
     5101 cgatgaatta acgcatcatc acaatcaaat cggctctctc catattcgag cgaccataca
     5161 ccacacgttg ctttttctgc cttggcacgc agtgcctgat agtcaatcag gctcactggt
     5221 tgcctccttt gcgaatctgt tccgcccatt cttcaaggga tttctccgca tattcaccag
     5281 acaggccatc aatcggatgc ggttcattag ccaactcttc tttcgctgac agaatcatgc
     5341 gcgtaacatc gaaaacttca cgtaaagact tattgataaa tccgtggttg aaagcagcag
     5401 caagacggct tgcggcatag ttaataccct cgttgcgtgc ttccgcacgt acttcagcca
     5461 ggaaagcatc ggtggctggg gttttgattt cgttaagcgc atcactgaat aaaccacgct
     5521 ccatacctag ctctgcttcg taatcggcat cgaatgcagc gtctttgcag aacttcttca
     5581 tccccgcact ctccgctgcc agcgccgcgc acttggcctc aaggttatca atcgtgattc
     5641 cagcagaacg acactcccgc aacgccgttt ccagttttga ttcaagttca ccgaacttac
     5701 ggacaagata ttcagcgttt gtttcgttaa cctttagatc tcgtgggatg catttacctt
     5761 tcagaaaacc atccatctca attagtgaca tttgtttcat tttttcccac tccgccacat
     5821 agcattcaga tatttgttgt cattaacaga accgaaactc tttctcttaa gcaattactc
     5881 tctcgatggc attggcttt
//
LOCUS       DAZFWL020000213         5871 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFWL020000213 DAZFWL020000000
VERSION     DAZFWL020000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.214"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..161
                     /locus_tag="ACHKNP_RS23455"
                     /old_locus_tag="pgaptmp_004691"
     CDS             <1..161
                     /locus_tag="ACHKNP_RS23455"
                     /old_locus_tag="pgaptmp_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HGU4706707.1"
                     /translation="QITLPVILKNERQINVFLRTEDIDLINVINEETLLQQPEERFAW
                     LRSKKDRF"
     gene            233..1591
                     /gene="mltD"
                     /locus_tag="ACHKNP_RS23460"
                     /old_locus_tag="pgaptmp_004692"
     CDS             233..1591
                     /gene="mltD"
                     /locus_tag="ACHKNP_RS23460"
                     /old_locus_tag="pgaptmp_004692"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HGU4706708.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1639..2409)
                     /locus_tag="ACHKNP_RS23465"
                     /old_locus_tag="pgaptmp_004693"
     CDS             complement(1639..2409)
                     /locus_tag="ACHKNP_RS23465"
                     /old_locus_tag="pgaptmp_004693"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4706709.1"
                     /translation="MTIQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALID
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2487..3287)
                     /gene="yafD"
                     /locus_tag="ACHKNP_RS23470"
                     /old_locus_tag="pgaptmp_004694"
     CDS             complement(2487..3287)
                     /gene="yafD"
                     /locus_tag="ACHKNP_RS23470"
                     /old_locus_tag="pgaptmp_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU4706710.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3528..4442
                     /gene="yafC"
                     /locus_tag="ACHKNP_RS23475"
                     /old_locus_tag="pgaptmp_004695"
     CDS             3528..4442
                     /gene="yafC"
                     /locus_tag="ACHKNP_RS23475"
                     /old_locus_tag="pgaptmp_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HGU4706711.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIVRGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4439..5242)
                     /gene="dkgB"
                     /locus_tag="ACHKNP_RS23480"
                     /old_locus_tag="pgaptmp_004696"
     CDS             complement(4439..5242)
                     /gene="dkgB"
                     /locus_tag="ACHKNP_RS23480"
                     /old_locus_tag="pgaptmp_004696"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HGU4706712.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAVIGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5405..5481)
                     /locus_tag="ACHKNP_RS23485"
                     /old_locus_tag="pgaptmp_004697"
     tRNA            complement(5405..5481)
                     /locus_tag="ACHKNP_RS23485"
                     /old_locus_tag="pgaptmp_004697"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5445..5447),aa:Asp,seq:gtc)
     gene            complement(5675..5790)
                     /gene="rrf"
                     /locus_tag="ACHKNP_RS23490"
                     /old_locus_tag="pgaptmp_004698"
     rRNA            complement(5675..5790)
                     /gene="rrf"
                     /locus_tag="ACHKNP_RS23490"
                     /old_locus_tag="pgaptmp_004698"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1539 a         1415 c         1436 g         1481 t
ORIGIN      
        1 atcaaataac actacccgta attctgaaaa atgagcggca aattaatgtt tttttaagaa
       61 cggaagatat tgatttaatt aatgtaatta atgaagaaac attattgcaa caacctgaag
      121 agcgttttgc atggttaagg tcaaagaaag ataggttctg ataaaacttt cttgtcatcg
      181 gctccgttcg ccgttatgat cggtcgtctt ttaagcaact attgacacac acatgaaggc
      241 aaaagcgata ttactcgcct ctgtcctgct cgtgggttgc cagagtaccg gcaacgttca
      301 acagcacgca cagagccttt ctgcagctgg tcaaggggaa gcagcaaagt ttacaagtca
      361 ggcacgatgg atggacgatg ggacgtctat cgcgccagat ggtgacttgt gggctttcat
      421 tggcgacgag ctaaagatgg gaattccgga aaatgaccgg attcgcgaac agaaacagaa
      481 atatttacgc aataagagct atctccacga tgtaacttta cgggcagagc cgtatatgta
      541 ctggatagca gggcaagtta aaaaacgtaa catgcctatg gaactggtac tactacccat
      601 agtggagagc gcttttgatc ctcacgcaac gtctggcgcc aatgccgcag gcatctggca
      661 gatcattccg agcacggggc gcaattatgg tttgaaacag acccgcaatt atgacgcgcg
      721 tcgcgatgtt gttgcttcaa caactgccgc gctgaatatg atgcagcgtc tgaacaagat
      781 gtttgacggc gactggcttc tgaccgtagc ggcttataac agcggcgaag gtcgagtcat
      841 gaaggcaatt aaaacgaaca aagcgcgtgg gaaatccacg gacttctggt cgttaccgtt
      901 gccgcaggaa acgaagcagt acgtgcctaa aatgctggca ttgagtgata ttctcaaaaa
      961 cagcaagcgt tatggcgtac gtctgccaac gaccgatgaa agccgtgctc tggcgcgtgt
     1021 gcacctgagc agcccggttg aaatggcgaa ggttgcagat atggcgggga tttccgtcag
     1081 caagctgaag acattcaacg ctggcgtgaa aggctccacg ctgggcgcaa gtggtccgca
     1141 gtacgtgatg gtgccaaaga agcatgcaga tcaactgcgt gaatctctgg cttcaggcga
     1201 aattgctgct gtacagtcga cgctggttgc cgacaatacg ccgcttaaca gccgtgttta
     1261 caccgtacgc tctggcgaca cgctttcaag tatcgcttca cgtctcggcg taagcaccaa
     1321 agatttgcag cagtggaaca aactgcgcgg atctaaactg aagccaggcc aaagtttgac
     1381 gattggcgca ggcagtagcg cacagcgact ggcaaacaac agcgatagca ttacgtatcg
     1441 tgtgcgcaaa ggcgattcgc tttcaagcat tgctaaacgc cacggcgtga acatcaaaga
     1501 tgtgatgcgc tggaacagcg acactgcgaa tctgcaacca ggcgataagc tgacgttgtt
     1561 tgtgaaaaac aacaacatgc cagattcctg acaaaccaga taataaaaag gcaccgattc
     1621 ccccggtgcc ttttttattt atgccgcttt atgtgcttct accatgatga tatcactggt
     1681 gaaagagccg tcattctgca aggcaaaata cgttctcacc tctgtcgatg cgctctgttg
     1741 gtaaatgcga atagcgtcta ttaacgcttc tggcgtacgc attctcacga cccatgaaga
     1801 aaattccagc ggtaacttat ctgtaattaa attatcaact atcagattgg cttcattgat
     1861 taacgtcaac cactcaccgc tggcgtagtt tcgtacgtga gaggtatcgc gtaatgcttc
     1921 taccgtctgt aaccagatgt cgcgcactgg gtgacccgga gacattacgt ccatcacaat
     1981 cagcctaccg ccaggtttca atatccggtt cacttcacgc agtgctgcac caacatcatg
     2041 ccagtgatgg gcagaataac ggctgataac aatatcaaat gcgttgtcgg caaatggcag
     2101 actttcggca taaccctggc gggtggtgat atttttcagt tgccggactt cggcagcttg
     2161 tgccacgaca tctagcatgt gggcagataa gtcatacgcc accaccgtgc tcacgttttg
     2221 cgcagcgaca aagctggcat gccctgctcc gcagcccata tcaagtacac ttgcaccagg
     2281 ataatcagcc agacgcaccg ccaggcgctg taaatctcgt ccggatgcat gtacggtact
     2341 ggtcagatat tcgctggctt gagagctaaa ctgcttttct acatggtcat ggtgggattg
     2401 tattgtcatt gcgctgtcct tttgttattt gagtataaaa gggcagcgcc aaacgttggt
     2461 ctgccctgtg gcagacctga cataccttat ttatcaggct tgccgggact gaattcaacg
     2521 agtagcggat tgtgatcgga agcgcgcgta accagtacag aagcttcgct gacgttcaga
     2581 ccacggtaga aaacaaaatc gagcgggcga ccaaacgccc gacggcgctg atcatcggta
     2641 aaacgcacct ggcgcagcga catttcccgc gcaaagcgat ataacgcgtt catccttcta
     2701 cggctccagg cattgaaatc tcccgccata atgaccgggc cgctgtggtg agctatctga
     2761 tcaccaatag gaagtaactg cttactatag acatccacgc ccagactgaa gttgacggcg
     2821 tgtatattaa ccaccatcaa caggcgggtg tcaggtaatg gatagaccgt caccagtgcc
     2881 gacttcgcca gacgcaaaat gggttctcgt tcacgtaacg ggcagcaata cactggatgc
     2941 gccgccgaaa gggtcattac accggaagga tgttgtggca gcacgaaagc gggcacctga
     3001 tcggcggcaa gatagttagc ggtcgcaaac tgtactaact ctggcgttgt ctgcgcttcc
     3061 tgcaataaca ccagatgtgc atctttgccg tagttcttta ataccgacaa ccattcagcg
     3121 cgttgctgtt tgtatatgtt ccacaccagg atccgaatac gctcttcagt acttaacggt
     3181 tctccaggtg gtaatgcctg gccgatgctc gcaaaagacc ccggcggtaa gatcctttcc
     3241 gcaggttgtc cggcaacata gcgcatggca taggtgtttt ttcgcacttt tgactttgaa
     3301 acctctatta cgtcaaccct tccgcactcg ggagggttct actgttatag ggattttagc
     3361 tcactctttc aacgagcaat tgtatattgt tatgtaagca agtgcttaca caggtcagaa
     3421 ttttgctcat tattgagaac attaaatcgg atgacatatc acgcatcctg atggtttaat
     3481 gacaaataaa ttcaaaatat ctcttctatt ttgcaacagg agcaaaaatg aaagccacgt
     3541 cggaagaact cgccattttt gtttcggtcg tagaaagcgg cagctttagc cgggcagcgg
     3601 aacaattagg gcaagcaaac tcagcggtaa gccgggcagt gaaaaagctg gagatgaaac
     3661 ttggcgttag cctgcttaat cggaccacgc gacaacttag cctgacggaa gaaggccagc
     3721 gttattttcg tcgcgtacag tcaattttgc aggagatggc agcggcagaa tcagaaatta
     3781 tggagacgcg taatacaccg cgtggactgt tacgcatcga tgccgcaact ccagtggtgc
     3841 tgcactttct gatgccgtta attaagcctt tccgtgaacg ctatccggaa gttactttat
     3901 cgctagtctc ctccgaaacg attattaatt tgatcgaaag aaaagtggat gtcgcgatac
     3961 gcgctggtac gttaacggat tccagcttac gtgccaggcc gttatttaac agttatcgaa
     4021 aaattatcgc ctcccccgat tatatttccc gctacgggaa gccagaaacg atcgacgatt
     4081 taaagcaaca tgtttgcctg ggattcactg aacccgcttc cctcaatacc tggccgatag
     4141 cctgtagtga tggacaatta catgaggtga agtacggttt gtcatccaat agtggggaaa
     4201 cactgaaaca gctttgcctg agtggcaacg ggattgcgtg tttgtccgac tacatgatcg
     4261 acaaagaaat cgttcgcgga gaattggtgg agttaatggc agataaagtg ttgccagtgg
     4321 aaatgccatt cagtgcagtc tattacagcg accgtgcggt aagtacgcgc atccgggctt
     4381 ttatcgattt ccttagcgag catgtaaaaa cagctcccgg aggagctgtc agagaggctt
     4441 aatcccattc aggagccaga ccttccgggc taaccaggcg gtcgttgcaa tccagtgcgg
     4501 cgatcgcttt tttatcttcg gcatcaagct gtaaattttg tgccttaaga ttactttcca
     4561 ggttttcacg tttagtagaa gaaggaatta ctgagtaacc ttcccccata gcccacgcca
     4621 gaatcacttg tgccggagtc gcattgtgtt tagctgcgat acgagcaata acctcatctt
     4681 tcaaggcttt cccataagcc agcgtcatat aggaagtaat atggatgccg tgctgtttag
     4741 cccaggcaac cactttacgg ttttgcagat aaggagagag ttcaatctgg ttagtagcga
     4801 tgttttcagc gccaataaca gcaatcgcct tttccatcaa tgggatcgtg aagttggaaa
     4861 taccgatctc acgcgtcagc ccttgttttt tggcttccag cagcgcctgc ataaactctt
     4921 caacagagac ttcatcgttt ggtgacggcc agtggattag cgtcagatca acataatcgg
     4981 tacgcaattt ttgcaggctc tctttcagac ttgggatcaa tttgtctttg ctgagatttt
     5041 caatccagat tttagtggtg atgtagagtt catgacgtgg cacgccactt tctgcaatcg
     5101 cctgacctac tgcggcttcg ttatcataga tttgtgcggt atcaattgcg cgataaccaa
     5161 gttcaagcgc cgttttcaca gatgaaataa caacgtcgtc tttcagacgg aaagtaccta
     5221 aaccaaatgc agggatagcc ataatatgcc tcttttaatg ctattttcgt taactgaggt
     5281 gattatgggc ttcagactgt tgaggaaaaa gagcgtaaaa tgcagaggat ttttgcgatt
     5341 ctggcaataa tagatatcca gacgaaaaaa aacctcgagc gttaactcga ggcttcttaa
     5401 taagtggcgg aacggacggg actcgaaccc gcgaccccct gcgtgacagg caggtattct
     5461 aaccgactga actaccgctc caccgaattc ttttacaacc accggtttta tgaccggctt
     5521 actgcttaat ttgatgcctg gcagtttatg aatcacttcg tgattcaccc ttcgggccgt
     5581 tgcttcgcaa cgttcaaaag ctttcgcttt tgtcctactc tcttatcgag tagtgaactg
     5641 cctcaaagat cagtgccatt cttcgtaatt tgatgcctgg cagttcccta ctctcgcatg
     5701 gggagacccc acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc
     5761 aggtgggacc accgcgctaa ggccgccagg caaattctgt tttatcagac cgcttctgcg
     5821 ttctgattta atctgtatca ggctgaaaat cttctctcat ccgccaaaac a
//
LOCUS       DAZFWL020000214         5838 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFWL020000214 DAZFWL020000000
VERSION     DAZFWL020000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.215"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(210..707)
                     /locus_tag="ACHKNP_RS23495"
                     /old_locus_tag="pgaptmp_004699"
                     /pseudo
     CDS             complement(210..707)
                     /locus_tag="ACHKNP_RS23495"
                     /old_locus_tag="pgaptmp_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            complement(704..880)
                     /locus_tag="ACHKNP_RS23500"
                     /old_locus_tag="pgaptmp_004700"
     CDS             complement(704..880)
                     /locus_tag="ACHKNP_RS23500"
                     /old_locus_tag="pgaptmp_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706713.1"
                     /translation="MADFGSTKYSVSFEEWHELLMEYAELRGGSAADAEAWRDDYEAG
                     KNPVEAYCDEWGDE"
     gene            complement(873..1055)
                     /locus_tag="ACHKNP_RS23505"
                     /old_locus_tag="pgaptmp_004701"
     CDS             complement(873..1055)
                     /locus_tag="ACHKNP_RS23505"
                     /old_locus_tag="pgaptmp_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706714.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIHDEAKDIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            complement(1089..1301)
                     /locus_tag="ACHKNP_RS23510"
                     /old_locus_tag="pgaptmp_004702"
     CDS             complement(1089..1301)
                     /locus_tag="ACHKNP_RS23510"
                     /old_locus_tag="pgaptmp_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706715.1"
                     /translation="MLNTQKAINAEKYNEWARKFSEQIFKITGDENAAKNELEPWTPE
                     GNAPNYCWWEVDPVDVANEAMSYHND"
     gene            complement(1352..1708)
                     /locus_tag="ACHKNP_RS23515"
                     /old_locus_tag="pgaptmp_004703"
     CDS             complement(1352..1708)
                     /locus_tag="ACHKNP_RS23515"
                     /old_locus_tag="pgaptmp_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706716.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRKNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            complement(1686..2147)
                     /locus_tag="ACHKNP_RS23520"
                     /old_locus_tag="pgaptmp_004704"
     CDS             complement(1686..2147)
                     /locus_tag="ACHKNP_RS23520"
                     /old_locus_tag="pgaptmp_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HGU4706717.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIAGLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELDAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            complement(2144..2440)
                     /locus_tag="ACHKNP_RS23525"
                     /old_locus_tag="pgaptmp_004705"
     CDS             complement(2144..2440)
                     /locus_tag="ACHKNP_RS23525"
                     /old_locus_tag="pgaptmp_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGU4706718.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLTPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAR"
     gene            complement(2437..2829)
                     /locus_tag="ACHKNP_RS23530"
                     /old_locus_tag="pgaptmp_004706"
     CDS             complement(2437..2829)
                     /locus_tag="ACHKNP_RS23530"
                     /old_locus_tag="pgaptmp_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4706719.1"
                     /translation="MAKEFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            complement(2845..3570)
                     /locus_tag="ACHKNP_RS23535"
                     /old_locus_tag="pgaptmp_004707"
     CDS             complement(2845..3570)
                     /locus_tag="ACHKNP_RS23535"
                     /old_locus_tag="pgaptmp_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4706720.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMVISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVREAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDIVRQITDSSRRVVSEK"
     gene            complement(3604..4065)
                     /locus_tag="ACHKNP_RS23540"
                     /old_locus_tag="pgaptmp_004708"
     CDS             complement(3604..4065)
                     /locus_tag="ACHKNP_RS23540"
                     /old_locus_tag="pgaptmp_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4706721.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKLLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            complement(4058..5068)
                     /locus_tag="ACHKNP_RS23545"
                     /old_locus_tag="pgaptmp_004709"
     CDS             complement(4058..5068)
                     /locus_tag="ACHKNP_RS23545"
                     /old_locus_tag="pgaptmp_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HGU4706722.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNSDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAVQEIRAAREQWERENGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            complement(5155..5592)
                     /locus_tag="ACHKNP_RS23550"
                     /old_locus_tag="pgaptmp_004710"
     CDS             complement(5155..5592)
                     /locus_tag="ACHKNP_RS23550"
                     /old_locus_tag="pgaptmp_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4706723.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            complement(5589..5831)
                     /locus_tag="ACHKNP_RS23555"
                     /old_locus_tag="pgaptmp_004711"
     CDS             complement(5589..5831)
                     /locus_tag="ACHKNP_RS23555"
                     /old_locus_tag="pgaptmp_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4706724.1"
                     /translation="MKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIPACEALD
                     WGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
BASE COUNT         1295 a         1682 c         1201 g         1660 t
ORIGIN      
        1 aggcggcctc ctgatgtttt gagggtgcag aaatccctcc ggttaaggat taaattttta
       61 actaaattta attattcagg cgcgataagc tgttccgcac aatccagcaa tgcttctgtc
      121 gcttccttaa gcgttactgt atcgctatca tccagtcctg caactttttc gtacctgaca
      181 aacgccgcgc aaagctcgtt aaacaacccc gcccgtactt cagccaggaa agcgtcggtg
      241 gccggagttt caggaatccg tctcctcatc cgttctattg catgattgaa cccgaaatct
      301 tcagcaagag atacgtcatc catattgtca ttgtcgtcct caatatcccg tggttctgga
      361 attgcagact ttattaccgc attctccgcc gccagcgcca caagattagc tttcagctct
      421 gcaattcgtt tgttttgggc ttcccgttca tccagtagtg ccagcgcaac atttggatta
      481 aaagcagcaa taaattcagc gtttgcataa gcctgaacat ctgtttcaac caggcagtta
      541 acatgacatt ctgcaattac gccaccgggc tctcctttcc atttttgaca aacaaaaact
      601 cctgttatat tcccatgctg attgcccgat gtatgcccta cgatgtagca tcctttagtt
      661 gctttctctg ctgcttcacg cagcgcctga tagtcaatct cgctcattca tcgccccact
      721 catcacaata tgcttcgacc ggattttttc ctgcttcata atcatcacgc catgcttcag
      781 catcagcagc actgccacca cgtaactctg catattccat taacagttca tgccattctt
      841 caaaactgac gctgtattta gttgaaccaa aatcagccat tttgttcttc ctcttcgtct
      901 tttatttcgt gatatgaata attgcagtag ttaaagaaaa tatcttttgc ttcgtcatgt
      961 atttcatcag gcgtcgcatc atcatccact tcgaattcat cctcgaaatc tccaccggct
     1021 attcccgttt caataattat tttaaacttt cgcatttaac taccgccctt tcgggcggcc
     1081 tcctgacatt aatcgttgtg gtaactcatg gcttcatttg caacatcaac cggatcaacc
     1141 tcccaccagc aataatttgg tgcgtttcct tcaggtgtcc acggttctaa ttcatttttt
     1201 gccgcattct catcgccagt aattttaaaa atctgctcag agaattttct tgcccactcg
     1261 ttatattttt ccgcattaat ggctttctgt gtatttaaca taaatatacc tccagttcag
     1321 gattagattt tatttacagc gctaaattta tttattcagt tctggatttt gtcgccctgc
     1381 gtatccgcgc tttcgcgtta cgttcaatct gaattaactt ttctatattt ttccgccttt
     1441 cctgttcctc ctggcgcaat agccttacat catctgccag tctggtttct cttttcgcca
     1501 cagagagcat ccagtcaaac ggctccacaa ccgcaccgca gattttacag cggacctggc
     1561 gctctttttc gtcaacccga acagaggcgt gatgacaata tggtctttcc gatggctcat
     1621 aaagaaaatt aacctgatta cgagggtcat cctcttttac cggaaataaa acgatattgc
     1681 ttaactcatc ctctggtttt atttccatgc tcctctcctt tgatgcgaat gccagcgacg
     1741 cgtaatgcgt gttctaggtc aatcaggtaa agccaactgc cattttcttt aggtatcatg
     1801 acatgtcgct catctgcatt tatcgggtgt ccatatcgaa ggtcgtagcg agtcggtaat
     1861 tgaacttccc gcgcatccag ttcagcaata cgcttgctcc catcagagat aacgccttcg
     1921 taatactcac gctgctcgtt gagttgtgat tttgcttctt ccagtccatc cagcaaacca
     1981 gcgataatat ccgcttcccg atgacggata tgacgcttaa acgcagcaag agccgcatca
     2041 caatcccgtt cagcatttgg gctgtccggg gtagcctgat accacgccag cgtcgactga
     2101 tagttttgtg ctgcctcacg aagcgcctca tagttaacct ctctcatcga gccacctcct
     2161 gataaatcac cgcatgcccc agtttctccg ccagtgccag ctctgcctta gcgcccgctg
     2221 accgctgcca gccattcagc atgtaaatcg catccacaca acgaatcatt gccatgcaaa
     2281 tatccatgta gtgcggctgt gtcagcccgt ccggaagtac tgccggagtt aagacggtat
     2341 gcccttcccg tttcagttcc tcttccgcct tgtgaaacgc ctcacggttg aaattttcat
     2401 atcccgtcat tggaccggca atataaactc tcaccctcac tccatcacct cctgaaagtt
     2461 tccccgatag aacgccagca cacgctgcat aacttcgctc tggcggcact cacgacaaat
     2521 tatgttctgc cgtctgttgt aacgacgtat ttctccgtca ggtaactttc gaatcagtgt
     2581 cgggtcagca gccttctccg gtgtcttacg ccatacgcga tacgcctgct ctgatggaaa
     2641 taccccgcaa ccagagagcc agacatcacc actggccgca agcgcaccag ataaacgacg
     2701 aatagcggtc ttactgacac ccgttttatc tgccagttgt cgaaaagttt ctcgtccgct
     2761 caggcgcacg aattccacaa tgcgcgcctt cacttcttcc cgctcttctg gtgtaaattc
     2821 ttttgccata agcgcctccg gcaatcactt ttccgataca acacggcggg aagaatcagt
     2881 aatctgtcga acaatatccc ggtgcttgtt cagctcccgc agcgcggcgc agactcgctc
     2941 ccacttctga acctgacctt ttgcccggcg cagctcgcgg ttagccacat gcagcgatgg
     3001 taaaatcaga ccatccggat gttttctggt gaacgacggc tgtgactgca ctgtgaccgc
     3061 cacactttcc gtttttattt cttcctgtgt ttctgcttcc cgaactggta acgcaacacc
     3121 tgctggctga ggaaaggctt taccatcggt ttccgctacg gatgcagctt ccggctctgc
     3181 cggtaaatca gcgcccggta tgcagtaacg aaatttaccg ccctgattca cgcgaatcag
     3241 acgccctttg ctgattacca tggccagcga tgaattcgcc cggcgggagg taatcccgaa
     3301 catcagtgcc agctcatccg ccgtttgtgg gccatgttgt tcaatcgcct cagtcagcat
     3361 ttgcgctgtc actttcggta ccggtgacac cggttcactt tcaccagcct gaatcagcca
     3421 ccacatcgac cccttgttat ccgcttcacc gcggcgcttc agtttccaca gttcgttgac
     3481 cgcatcttca cggctgattc caaggcgggc cgccactacc tgtgaagagg ctcttttcag
     3541 tgctttcagt gcgtcaaata cggtttccat taaaatttcc tccgacaaaa tcgtttctca
     3601 gattcaaata aaaccagctg ccttccggcg ttcgtattcc tgtttcagcc gctcaattgg
     3661 cgttggccct tgcgggtgtt tcgccccttc cagttgtcgt cgcactggcg gaacactcat
     3721 cccgttacca acatgctttg cccatttcgt cagttgccgt tctgcaagta gttttaactc
     3781 accctgcgtc atctggcgct caatccctct ggtacgcatt tcgaggcaga tgtggtacag
     3841 cacaggctgt ggccacgggt atttatcact cccgtcgtat cgccaggatt cattgcgcca
     3901 gcgccggtac tcttccatca cggcatccac cgtaagacca aatggatttg ccccactctc
     3961 cgaaatcagc gcaacaaact cagccaggtc cgggggccac gtttcacccg cccggcagcg
     4021 gtccatgcac tgacggcaga ccagacggat ttgctgttca gtcatcgcac caatctgggc
     4081 aatccagagc ttcgaaggtg cggccccgtt cttctgagtc cagcggttcg aatacacctc
     4141 ccccataagc tcccacagct tccaggccgt ttccgttgct gataaatccg ttttcacgtt
     4201 cccactgttc gcgtgctgcc cggatttcct gaactgcccg tgatgccgtg ccacctgatg
     4261 ctgcatggct tacccccttg ctgactggtt ttacctgtgc cctgacgtgc tgcacgtggc
     4321 gggcaaattt ctgctcccac tgaacctgcg tgaaaacctt cccctccgcc atccagtaat
     4381 cccggaatgc ggcaagctca gcaggtgtaa attccggctc aggcagagcc ataccccaca
     4441 ctgctgcccg ttgtcgaaaa tccggcgacg gctgccagac agtagtcatc gaaaatttcc
     4501 cgatcggttc gctcaggccg tccaggtatt caggttcggc tgtctgcaac ggcgcaccat
     4561 gcgactcact ggtcggaata ctctcgcgtt cacgcacgtt atgtgtgggg tttaattctt
     4621 ttagatctgt atctttatta gttgcttttg tgtttgcgtc atgttcaaac acaacaccaa
     4681 catttgtttg aacacctgtt aaatctctct cttgttttgt ttgaacatat gcttcctttc
     4741 tgcttcttct ggcctgaaca gatgcttttc cggcggctga ttttttggtc aatttttccc
     4801 tgactgatgc cagatcttcc tcaatccgaa gatgcaccca ttcatcgctg ttatcgcaaa
     4861 aaaactcccg caaggatggt tccacatcag cccatcgctc gttagtcaga cgggaaattt
     4921 ttgccagcct gtttttaggt attggtttcc ctgtttgcca gtaattgaac atcagcaaca
     4981 aatacgcacc gtgctcctct gcggacaaat gcatggtgtc agccaggtaa tcagctatgt
     5041 acagttgcat gtatggtaat gcggccataa ttgcctcatc ttgtgacgaa ccatcctctg
     5101 gtgatattct gtgattcccc aatcaacaga atcagcaggg gtctggcata aatatcaatg
     5161 caccacaaca gactcgccgg atgacccgcc gtcgctgaaa tacgctttcc ggtaaactgc
     5221 ctggactgca tcatcatgcg catcaattgc cgtgctcaac gcttcctggg ccgccagtaa
     5281 tgcacggcgc tcaacagtgt caaaaatact gaggtgataa cgcagttcac gcggaagaac
     5341 agtcaggata gctggaatta gtgcctgaat tttttcaaca gcatcaggcg tatccttttc
     5401 aagccagcga aaaatatttt gtatgttcaa tccaataccc tctgtcgtag aggtgtcatg
     5461 cattggtgga taagtcattt caagctcaaa atatgctgtc gctatatcat cagcgatttt
     5521 tttgcgccct accctcggat aaagcagcca cgcattcatc gccatgcgga tgtgctcatg
     5581 cttgattttc atgaatcaac tcccgccgct gcttgtgcgt tagcctgata ctcaacaggt
     5641 aaaccatcgg ttggattagg gtatttatca gggcgcaatt catgcggagt tactccccag
     5701 tcaagcgcct cacatgctgg tataacctcc tccgctggca ctcgtttttt aaaccaccca
     5761 cttatggttt gcggtgtttt accaagacga cgccctaatt ctgattggct cattattgac
     5821 aggattttca cttgagta
//
LOCUS       DAZFWL020000215         5837 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFWL020000215 DAZFWL020000000
VERSION     DAZFWL020000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.216"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            25..1176
                     /gene="yiaY"
                     /locus_tag="ACHKNP_RS23560"
                     /old_locus_tag="pgaptmp_004712"
     CDS             25..1176
                     /gene="yiaY"
                     /locus_tag="ACHKNP_RS23560"
                     /old_locus_tag="pgaptmp_004712"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HGU4706725.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQLLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1341..2879
                     /gene="aldB"
                     /locus_tag="ACHKNP_RS23565"
                     /old_locus_tag="pgaptmp_004713"
     CDS             1341..2879
                     /gene="aldB"
                     /locus_tag="ACHKNP_RS23565"
                     /old_locus_tag="pgaptmp_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HGU4706726.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3098..3301)
                     /locus_tag="ACHKNP_RS23570"
                     /old_locus_tag="pgaptmp_004714"
     CDS             complement(3098..3301)
                     /locus_tag="ACHKNP_RS23570"
                     /old_locus_tag="pgaptmp_004714"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706727.1"
                     /translation="MAFQQVEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSIIYRLL
                     LIGLGTPMVIYGLVCPLTIETRE"
     gene            3424..3747
                     /locus_tag="ACHKNP_RS23575"
                     /old_locus_tag="pgaptmp_004715"
     CDS             3424..3747
                     /locus_tag="ACHKNP_RS23575"
                     /old_locus_tag="pgaptmp_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU4706728.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3753..4889
                     /locus_tag="ACHKNP_RS23580"
                     /old_locus_tag="pgaptmp_004716"
     CDS             3753..4889
                     /locus_tag="ACHKNP_RS23580"
                     /old_locus_tag="pgaptmp_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4706729.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYKRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4886..>5837)
                     /locus_tag="ACHKNP_RS23585"
                     /old_locus_tag="pgaptmp_004717"
     CDS             complement(4886..>5837)
                     /locus_tag="ACHKNP_RS23585"
                     /old_locus_tag="pgaptmp_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4706730.1"
                     /translation="RELKIISVIAASENISHAATVLGIAQANVSKYLADFESKVGLKV
                     FDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPTGIITYL
                     SKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESLVASFIT
                     QYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRV
                     KGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWWLDLVAIFR
                     KREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1435 a         1400 c         1534 g         1468 t
ORIGIN      
        1 tttttacact ttcaggagtg tgttatggca tcttcaactt tctttattcc ttctgtgaat
       61 gtcatcggcg ctgattcatt gactgatgca atgaatatga tggcagatta tggatttacc
      121 cgtaccttaa ttgtcactga cagtatgtta acgaaattag gtatggcggg tgatgtgcaa
      181 aaagcactgg aagaacgcaa tatttttagc gttatttatg atggcaccca acctaacccc
      241 accacggaaa acgtcgccgc aggtttgaaa ttacttaaag aaaataattg cgatagcgtg
      301 atctccttag gcggtggttc tccacacgac tgcgcaaaag gtattgcgct ggtggcagcc
      361 aatggcggcg atattcgcga ttacgaaggc gttgaccgct ctgcaaaacc gcagctgccg
      421 atgatcgcca tcaataccac ggcgggtacg gcctctgaaa tgacccgttt ctgcatcatc
      481 actgacgaag cgcgtcatat caaaatggcg attgttgata aacatgtcac tccgctgctt
      541 tctgtcaatg actcctctct gatgattggt atgccgaagt cactgaccgc cgcaacgggt
      601 atggatgcct taacgcacgc tatcgaagcg tatgtttcta ttgccgccac gccgatcact
      661 gacgcttgtg cactgaaagc cgtgaccatg attgccgaaa acctgccgtt agccgttgaa
      721 gatggcagta atgcgaaagc gcgtgaagca atggcttatg cccagctcct cgccggtatg
      781 gcgttcaata atgcttctct gggttatgtt catgcgatgg cgcaccagct gggcggtttc
      841 tacaacctgc cacacggtgt atgtaacgcc gttttgctgc cgcatgttca ggtattcaac
      901 agcaaagtcg ccgccgcacg tctgcgtgac tgtgccgctg caatgggcgt gaacgtgaca
      961 ggtaaaaacg atgcggaagg tgctgaagcc tgcattaacg ccatccgtga actggcgaag
     1021 aaagtggata tcccggcagg cctacgcgac ctgaacgtga aagaagaaga tttcgcggtt
     1081 ctggcgacta atgccctgaa agatgcctgt ggttttacta acccgatcca ggcaactcac
     1141 gaagaaattg tggcgattta tcgcgcagcg atgtaatcat catttccaca acggctggca
     1201 aattgtcagc cgctttttca actatctctg taacccttgc ccgtaaattc gtgatagctg
     1261 tcgtaaagct gttaccgact ggcgaagatt tcgccagtca cgtctaccct tgttatacct
     1321 cacaccgcaa ggagacgatc atgaccaata atcccccttc agcacagatt aagcccggcg
     1381 agtatggttt ccccctcaag ttaaaagccc gctatgacaa ctttattggc ggcgaatggg
     1441 tagcccctgc cgacggcgag tattaccaga atctgacgcc ggtgaccggg cagctgctgt
     1501 gcgaagtggc gtcttcgggc aaacgagaca tcgatctggc gctggatgct gcgcacaaag
     1561 tgaaagataa atgggcgcac acctcggtgc aggatcgcgc ggcgattctg tttaagattg
     1621 ccgatcgaat ggaacaaaac cttgagctgt tagcgacggc tgaaacctgg gataacggca
     1681 aacccattcg cgaaaccagt gctgccgacg tgccgctggc gattgaccat tttcgctatt
     1741 tcgcctcgtg tattcgggcg caggaaggtg ggatcagtga agttgatagc gaaaccgtgg
     1801 cctatcattt ccatgaaccg ttaggcgtgg tggggcagat tatcccgtgg aacttcccgc
     1861 tgctgatggc gagctggaaa atggctcccg cgctggcggc gggcaactgt gtggtgctga
     1921 aacccgcacg tcttaccccg ctttctgtac tgctgctaat ggaaatcgtc ggtgatttac
     1981 tgccgccggg cgtggtgaac gtggtcaacg gcgcaggtgg ggaaattggc gaatatctgg
     2041 cgacctcgaa acgcatcgcc aaagtggcgt ttaccggctc aacggaagtg ggccaacaaa
     2101 ttatgcaata cgccacgcaa aacattattc cggtgacgct ggagctgggc ggcaaatcgc
     2161 caaatatctt ctttgctgat gtgatggatg aagaagatgc ctttttcgat aaagcgctgg
     2221 aaggctttgc actgtttgcc tttaaccagg gtgaagtttg cacctgtccg agtcgtgcct
     2281 tagtgcagga gtcaatctac gaacgcttta tggaacgcgc catccgccgt gtcgaaagca
     2341 ttcgcagcgg taacccactc gacagcgtga cgcaaatggg cgcgcaggtt tctcacgggc
     2401 aactggaaac catcctcaac tacattgata tcggtaaaaa agagggcgca gacgtgctca
     2461 ccggcgggcg gcgcaagctg ctggaaggtg aactgaaaga cggctactac ctcgaaccga
     2521 cgattctgtt tggtcagaac aatatgcggg tgttccagga ggagattttt ggcccggtgc
     2581 tggcggtgac caccttcaaa acgatggaag aagcgctgga gctggcgaac gatacgcaat
     2641 atggcctggg cgcgggcgtc tggagccgca acggtaatct ggcctataag atggggcgcg
     2701 gcatacaggc tgggcgcgtg tggaccaact gctatcacgc ttacccggca catgctgcgt
     2761 ttggtggcta caaacaatca ggtatcggtc gcgaaaccca caagatgatg ctggagcatt
     2821 accagcaaac caagtgcctg ctggtgagct actcggataa accgttgggg ctgttctgat
     2881 ataagaagct ggtcgcattg ggtattcatt gcctgatgcg acgcttacgc gtcttatcat
     2941 gcctacggga acctgaccgt aggccggata aggcgtacac gccgcatccg gcataaacaa
     3001 agcgcacgtt gttacaattt acgttttaaa cctacgtgcg caataaccca gactacgcca
     3061 acagaagtgc gtttctgggg cgtgttttgc caggagttta ctctcgcgtt tcgatggtga
     3121 gcgggcaaac caggccgtaa atcaccattg gcgttccgag gccaatcagc aataaacggt
     3181 agataattga aggtttaata cgctttatct gtactgtgga tgttttactg gagttattca
     3241 ttagcttggt caatagttcc attatttcaa tatgataaac ctcgacctgt tgaaaagcca
     3301 cgcagcacac ttcagaaagt atataaaaaa caatatgccg ttatataaat aaggaaattg
     3361 cactgcatca tgggatttca taatatgcgt taattgttct tttttatatt ctctgaggga
     3421 aatatgtttc tggattattt tgcactggga gtgcttattt ttgtatttct ggtgatattc
     3481 tatgggatta ttattttaca tgatattccc tacctgattg ctaaaaaacg taatcatcct
     3541 catgccgacg ccattcatgt tgctggttgg gtgagtcttt ttacgctgca tgttatctgg
     3601 ccatttttgt ggatttgggc cacgctttac cgcccggagc ggggatgggg aatgcaaaac
     3661 catgactcct ccgttgtgca actacaacag cgtattgccg ggctggagaa acagctcgcc
     3721 gacatcaaat cctcttctgc cgagtaatac ttatggattt actgattatt ttgacctatg
     3781 tggcttttgc atgggcaatg tttaagatct tcaaaattcc tgtaaataaa tggaccattc
     3841 ccacagcggc cctgggtggc atatttattg tcagtggttt aattctgtta atgaactata
     3901 accatccgta taccttcaaa gcgcaaaaag cggttatttc tattcctgtt gtcccacagg
     3961 tgacaggcgt ggtgatcgaa gtgacggata agaaaaatac gctgattaaa aaaggtgagg
     4021 tgctatttcg actggacccg acgcgttatc aggcgcgggt ggatcggctg atggcggata
     4081 tcgttaccgc agaacataaa cagcgggcgt tgggcgcaga gttagatgag atggcggcga
     4141 atactcagca ggcaaaggcc acgcgggata aattcgctaa agagtataag cgttacgcac
     4201 gtggcagcca ggcgaaagta aacccgtttt cagaacgcga tatcgatgtg gcgcggcaaa
     4261 attatctggc gcaggaagcc tccgtgaagt catcggcggc ggaacaaaaa cagatccaga
     4321 gccagctgga tagcctggtg ttgggtgaac attctcaaat cgccagcctg aaagcacagc
     4381 tcgcggaagc aaaatataac cttgagcaga cgatagtgcg tgcgccgagc gatggttatg
     4441 tgacccaggt actgattcgt ccgggtacct atgccgcgtc gctgccgcta cgtccggtga
     4501 tggtgtttat acccgatcag aaacgacaaa tcgtggcgca gttccgtcag aactccttgc
     4561 tgcgactggc tcctggcgac gatgcggaag tggtgtttaa tgctctgcca ggtaaggtat
     4621 tcagcggtaa gctggcagcc attagtccag ccgttcccgg cggagcttat cagtcgaccg
     4681 gcaccttaca gacgttaaac acagcgccgg gttcagatgg cgttatcgcg accattgaac
     4741 tggatgagca cactgatttg agcgctttac cagacggtat ttacgcccag gtggcggttt
     4801 actctgatca tttcagccat gtctcggtga tgcgcaaagt gctattacgc atgaccagct
     4861 gggtgcatta cctttatctc gatcattaat cttcgctttc cgccgcttgt tcaggccgca
     4921 gttgctgcga ctgagcaagc tggtggcgaa tttcacgcag catttcatcc agcacatact
     4981 ggacccgcca gggctggtat tcccgtttac ggaagatagc caccagatcc agccaccatt
     5041 catagggttg ctgaaagcag ggcaccagcg tgccgtcttg caggtcgctc tgcacacttt
     5101 tgtctggcgc aaacacaatc cccaggtgat ttcgtgccag ctccagcgca gattgcgtgt
     5161 tgtcgcaaac gtaatttccc ttcacgcgat aatcccgcac ctctttactc ccggcgacgt
     5221 tgaagcgcca gatattcgcg tcgtcgatca tcatcgagtc gatcaaaata caggagtgat
     5281 gttcgagttc gtcaggacgg ctaatgggat gtttctcaag atagcgctgg ctggcataag
     5341 cggttacggc atattgggta ataaaactgg caaccagcga ttcatctttt ggtggagcgt
     5401 agctgattaa aacatcacaa tcatcaggaa attcgacgcc ttcataaaag gcattacgct
     5461 caagattgca ggtttttagc gataaggtga tatcaccgat atctttaatt ttatcgatta
     5521 catgtttgga taaataggta attatgccgg ttggggcgta gatggtgacc cgaccacgtt
     5581 tctcatgctt ataatctgca ataaaattat taagttgctc gtttctgtcc agcatgtcgt
     5641 tgatgtacgg taacagcgcg gtgccaaaag gtgtgagcat cagctgccgg gtggttcggt
     5701 caaaaacttt taaacccact tttgattcaa aatcagcaag atatttgctg acgttggcct
     5761 gtgcgatgcc aagtacagtc gcggcatggc tgatattttc actggcagcg attaccgaga
     5821 taatttttaa ctcccgg
//
LOCUS       DAZFWL020000216         5789 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFWL020000216 DAZFWL020000000
VERSION     DAZFWL020000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.217"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            129..839
                     /locus_tag="ACHKNP_RS23590"
                     /old_locus_tag="pgaptmp_004718"
     CDS             129..839
                     /locus_tag="ACHKNP_RS23590"
                     /old_locus_tag="pgaptmp_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4706731.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            881..3532
                     /locus_tag="ACHKNP_RS23595"
                     /old_locus_tag="pgaptmp_004719"
     CDS             881..3532
                     /locus_tag="ACHKNP_RS23595"
                     /old_locus_tag="pgaptmp_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4706732.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVITVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            3546..4076
                     /locus_tag="ACHKNP_RS23600"
                     /old_locus_tag="pgaptmp_004720"
     CDS             3546..4076
                     /locus_tag="ACHKNP_RS23600"
                     /old_locus_tag="pgaptmp_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4706733.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4089..4592
                     /locus_tag="ACHKNP_RS23605"
                     /old_locus_tag="pgaptmp_004721"
     CDS             4089..4592
                     /locus_tag="ACHKNP_RS23605"
                     /old_locus_tag="pgaptmp_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4706734.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4651..5565
                     /locus_tag="ACHKNP_RS23610"
                     /old_locus_tag="pgaptmp_004722"
     CDS             4651..5565
                     /locus_tag="ACHKNP_RS23610"
                     /old_locus_tag="pgaptmp_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4706735.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1683 a         1243 c         1281 g         1582 t
ORIGIN      
        1 accaacattt attacaaacg cattaagtct ggaaacccgg cgactcgcca atgtattagc
       61 aacattaatc gattgtctga tgtaacggtt ttcccgctta tcctcttaac acatggagaa
      121 tgcatcagat gcaaactacg cgtaccccct actctatttc ctttatggcg acagtgttac
      181 ttttattgct ctttgcttgc catagcaccg ttgccaacgc agctgtcgca ctgggtgcga
      241 cgcgggtaat ttatccggca aaccagaaac aggttttatt gcctgtcacg aataatgatc
      301 ctgccagtgt ctatctcatt cagtcatgga tagaaaatgc aggtgaccaa aaagacaccc
      361 agtttgtcat taccccaccg cttttttcca tgcaaggtaa aaaagagaat acgctgcgga
      421 taatcaacgc tactaatcat cagttaccag gcgaccgtga aagcctgttc tgggtgaatg
      481 ttaaagctat tcctgctatg gaaaaagacc agaagaatga gaatacatta cagctggcaa
      541 ttattagccg cataaaaatg ttttatcgcc ccacgaacct ggcaatggca cctgaagaag
      601 cgcctgcaat gcttcggttt cgtcgcagcg gtagcaaact cacgcttatt aatccaacgc
      661 cctatttcat taccgtaaca aatatgaaag caggaaatag caatctccca aacaccatgg
      721 tgccgccaaa aggtgaagta tcggtggata tcacgcatgc tgcaaccggg gatatcagtt
      781 ttcagacgat caatgattac ggtgcattaa ctccgcgtat taaggcaacc atgcagtaag
      841 ccagtgacct aattaataat aaactcaatc ggaattaata atgacagcct ttcgtgccgc
      901 atttaaggca tatcgtatgc atcaggtgtt aataatgcct cgctttgcac ggcttaccat
      961 tgcccttggc ttagcaactg cggtatttcc cgttgacgca gagtattatt ttaatccacg
     1021 gtttttaagc aatgatcttg ctgaatctgt cgatttatcc gcctttacta agggacgcga
     1081 ggcaccacct ggtacatatc gggtcgacat ttatttgaac gacgaattta tgactagtcg
     1141 ggatattact tttattgcag atgataataa cgcagaactc atcccatgcc tgagtacaga
     1201 tcttcttgtc agtcttggta taaaaaaaat agcgttatta gacaataaag aacattcggc
     1261 agaaaaacat gtgccggaca acagcgcatg cacaccactt caggatcgtt tggccgacgc
     1321 atcaactgag tttgatgttg gtcagcaaca tttgtcactc tccgttccgc aaatttatgt
     1381 tggcagaatg gctcgcggct atgtttctcc tgatctgtgg gaagaaggaa taaatgctgg
     1441 gcttctaaac tatagtttta atggtaattc tattaataat cgtagtaacc ataatgcagg
     1501 aaaatccaac tatgcatatt tgaatttaca gagtggcatc aacattggta gttggcgact
     1561 gcgcgataac tcaacgtgga gttataacag tgggagtagc aattcatctg acagcaataa
     1621 atggcagcat atcaatacgt cggctgaacg tgacattatt cccttacgct cacggttaac
     1681 ggtaggtgat agttataccg atggcgatat ttttgatagt gtgaactttc gtggcctcaa
     1741 aataaattca acagaagcga tgttgcccga tagccaacat ggtttcgctc cggtgattca
     1801 tggtattgcc cgtggaaccg cacaagtgag tgtaaaacag aacggatacg atgtttatca
     1861 gactactgtt ccacccggcc cttttactat tgatgatatc aactctgcgg ccaatggtgg
     1921 tgatctgcaa gtaaccataa aagaggcaga cggcagtatt cagacattat atgttcctta
     1981 ttcgtctgtt ccggttctcc aacgtgctgg atatacgcgt tatgcgcttg ccatggggga
     2041 atatcgtagt ggaaataacc tgcaaagctc tcccaagttc atacaaggta gcttgatgca
     2101 tggtctggaa ggaaactgga caccttatgg cggaatgcaa attgcagaag attatcaggc
     2161 cttcaacctt ggtattggga aagatttagg actttttggt gccttttctt tcgatatcac
     2221 gcaggccaat acgacacttg cagatggcac ccgtcacagt gggcaatcgg ttaaatccgt
     2281 ctacagcaaa tccttctacc agacaggaac caatatccag gtcgcaggat atcgctattc
     2341 tacgcaaggt ttttataact tatccgacag tgcctacagt cgaatgagtg gttacaccgt
     2401 caagcctcct accggagaca ccaatgagca gacacaattt attgattatt ttaatctgtt
     2461 ctacagtaag cgtggtcagg aacaaataag catctctcag cagcttggaa attacggtac
     2521 gacatttttc agtgccagtc gccaaagtta ctggaacacg tcacgcagcg accagcaaat
     2581 atcatttgga ttaaatgtgc cgtttggtga tattacgact tcgctgaatt acagctattc
     2641 caataatata tggcaaaacg atcgggatca tttactcgct tttacgctta atgttccctt
     2701 cagtcattgg atgcgtacag acagtcagtc ggcatttcgt aattcaaacg ccagttacag
     2761 tatgtcaaac gatttgaaag gcggcatgac caatctatcg ggggtttatg gcactctgct
     2821 gccggataat aacctgaatt atagcgttca ggtcggtaac acccacggag gtaatacatc
     2881 gtctggcacc agtggttaca gttctcttaa ttatcgtgga gcttatggta atactaatgt
     2941 cggttacagt cggagtggtg acagcagcca gatttattac ggaatgagtg gtgggattat
     3001 tgctcatgct gatggcatca cctttggaca gccgctgggc gacacaatgg ttctggttaa
     3061 ggctcctggt gctgataatg tcaaaataga gaaccagacc ggaattcata ccgactggcg
     3121 tggctatgcc atattaccat ttgcgacaga atatagagaa aaccgtgttg ctcttaacgc
     3181 gaattccctt gcagataatg ttgaactgga tgaaaccgtg atcactgtca tcccaactca
     3241 cggtgctatt gccagagcaa catttaatgc acaaatcggc gggaaagtat taatgacgtt
     3301 gaagtacggt aataagagtg ttccattcgg tgcaattgtc acacacggag agaataaaaa
     3361 tggcagcatt gtcgcagaaa acggtcaggt ttatctgact ggacttccac agtcagggaa
     3421 attacaggtt tcatggggca aagataaaaa ctcaaactgt attgtcgagt acaagcttcc
     3481 tgaagtttct cctggtacct tactgaacca gcagacagca atctgtcgct aaggaacaat
     3541 agattatgaa atacaataac attattttcc tcggtttatg tctggggtta accacctatt
     3601 ctgctttatc cgcagatagc gttattaaaa ttagcgggcg cgtcctcgat tatggctgca
     3661 cagtctcatc ggattcgctt aattttaccg tagatctcca aaaaaacagt gccagacaat
     3721 ttccaacgac cggtagcaca agtccagccg tcccttttca gattacgtta agtgaatgca
     3781 gcaaagggac aacgggggtt cgggttgcat ttaacggtat tgaggatgca gaaaataata
     3841 ctttgttgaa actggatgaa ggaagcaata cggcctccgg tttgggtata gaaatattgg
     3901 acgcaaatat gcgtccggtg aaactgaatg accttcatgc cgggatgcag tggatcccac
     3961 tggtaccaga acagaacaat attttgcctt actccgctcg tctgaagtca actcagaagt
     4021 ccgtcaatcc gggactggtg agggcttcgg caacctttac ccttgaattt caataatcag
     4081 agtcagatat gaaaagactt cacaagaggt tcctgttagc tacgttttgc gcgttattca
     4141 cagcaactct ccaggccgcc gatgtcacta tcactgttaa tggtcgggta gtcgctaaac
     4201 cctgcactat tcaaaccaaa gaagctaacg ttaatctcgg ggatctttat acgcgcaatc
     4261 tgcaacaacc tggttctgca tctggctggc acaatattac tttgtcatta accgattgtc
     4321 cggttgaaac aagtgcagtg acggcaatcg tgacaggttc aactgacaat acgggttatt
     4381 acaaaaatga aggtactgcc gaaaatattc agatagagct gagggatgac caggatgctg
     4441 cgttaaaaaa tggcgatagc aaaacggtta ttgttgatga gatcactcgt aatgcacagt
     4501 ttccacttaa ggcaagagct atcacggtga atggaaacgc aagccaggga acgatcgagg
     4561 cgctaatcaa tgtgatctac acctggcaat aacgtcgatt cgattttctt agccttcttt
     4621 ttaaatcaat ttttaacctt aggatatatc atgggtaaaa caatcagtat aaaggtcctg
     4681 ttcggtattt atcttctatt aatggcaggc aaagtttttg ccttcagttg taatgtcgat
     4741 ggcgggtcaa gtattggagc aggtacaacc tctgtttatg ttaatctcga ccctgtaata
     4801 cagccgggcc agaatctggt tgtagacttg tctcagcata taagttgctg gaatgattac
     4861 ggcggctggt acgacactga tcatataaac ctggtacaag gctcagcttt cgcaggctcc
     4921 ctacagtcat ataaaggatc gctgtactgg aataatgtga cctacccctt cccgttgacg
     4981 acaaatacca acgtattgga tattggtgat aagacaccga tgccattacc gttaaaactc
     5041 tatattaccc cagttggggc ggccggtgga gttgtcataa aagcggggga agtcattgcc
     5101 cgtatacata tgtataaaat tgcaacatta ggaagcggaa atccgcgtaa ttttacgtgg
     5161 aatattattt caaataacaa tgttgttatg ccaacaggcg gctgcaccgt tgatagtcgt
     5221 aatgtcaccg tggatctccc ggactttcct ggaagcgcag aaatacccct tggcgtatat
     5281 tgctcaagcg aacaaaagct ctcattttat ctttcgggtg ccaccactga cagtagccga
     5341 caagtgtttg ctaacacagc acctgatgca acaaaagcat cgggagtggg tgtcactcta
     5401 atgcgtaatg gaaaaatact ggctacggga gaaaatgtct ccctggggac agtaaataaa
     5461 agcaaagtac ctctgggttt aagcgcaacc tatggacaaa caggaaacaa ggtttcagcc
     5521 ggaaccgtcc agtcagtcat tggtgtcact tttatctatg aataattgtg attttgacct
     5581 tagtctatgc gatccattca cgaaaattgc gcggataatt attttgtgaa ggctattagc
     5641 ctacacctgt aagaaaatcc gcgcacaatt gaagccagag tcttattttt tatattgaaa
     5701 aatatgttaa tttaaacact atgagagatg atgcttaact tatcgtattt aatctattgt
     5761 ttaacgataa tagattaaaa atagaaaga
//
LOCUS       DAZFWL020000217         5767 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFWL020000217 DAZFWL020000000
VERSION     DAZFWL020000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.218"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            114..2789
                     /gene="adhE"
                     /locus_tag="ACHKNP_RS23615"
                     /old_locus_tag="pgaptmp_004723"
                     /gene_synonym="adhC"
     CDS             114..2789
                     /gene="adhE"
                     /locus_tag="ACHKNP_RS23615"
                     /old_locus_tag="pgaptmp_004723"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGU4706736.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            2913..3809
                     /locus_tag="ACHKNP_RS23620"
                     /old_locus_tag="pgaptmp_004724"
                     /pseudo
     CDS             2913..3809
                     /locus_tag="ACHKNP_RS23620"
                     /old_locus_tag="pgaptmp_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(4091..4708)
                     /gene="tdk"
                     /locus_tag="ACHKNP_RS23625"
                     /old_locus_tag="pgaptmp_004725"
     CDS             complement(4091..4708)
                     /gene="tdk"
                     /locus_tag="ACHKNP_RS23625"
                     /old_locus_tag="pgaptmp_004725"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGU4706737.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5313..5726
                     /gene="hns"
                     /locus_tag="ACHKNP_RS23630"
                     /old_locus_tag="pgaptmp_004726"
     CDS             5313..5726
                     /gene="hns"
                     /locus_tag="ACHKNP_RS23630"
                     /old_locus_tag="pgaptmp_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HGU4706738.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLASVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
BASE COUNT         1544 a         1445 c         1347 g         1431 t
ORIGIN      
        1 cgcgagtaag cttttgattt tcataggtta agcaaatcat caccgcactg actatactct
       61 cgtattcgag cagatgattt actaaaaaag tttaacatta tcaggagagc attatggctg
      121 ttactaatgt cgctgaactt aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat
      181 atgccagttt cactcaagag caagtagaca aaatcttccg cgccgccgct ctggctgctg
      241 cagatgctcg aatcccactc gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg
      301 aagataaagt gatcaaaaac cactttgctt ctgaatatat ctacaacgcc tataaagatg
      361 aaaaaacctg tggtgttctg tctgaagacg acacttttgg taccatcact atcgctgaac
      421 caatcggtat tatttgcggt atcgttccga ccactaaccc gacttcaact gctatcttca
      481 aatcgctgat cagtctgaag acccgtaacg ccattatctt ctccccgcac ccgcgtgcaa
      541 aagatgccac caacaaagcg gctgatatcg ttctgcaggc tgctatcgct gccggtgctc
      601 cgaaagatct gatcggctgg atcgatcaac cttctgttga actgtctaac gcactgatgc
      661 accacccaga catcaacctg atcctcgcga ctggtggtcc gggcatggtt aaagccgcat
      721 acagctccgg taaaccagct atcggtgtag gcgcgggcaa cactccagtt gttatcgatg
      781 aaactgctga tatcaaacgt gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg
      841 gcgtaatctg tgcttctgaa cagtctgttg ttgttgttga ctctgtttat gacgctgtac
      901 gtgaacgttt tgcaacccac ggcggctatc tgttgcaggg taaagagctg aaagctgttc
      961 aggatgttat cctgaaaaac ggtgcgctga acgcggctat cgttggtcag ccagcctata
     1021 aaattgctga actggcaggc ttctctgtac cagaaaacac caagattctg atcggtgaag
     1081 tgaccgttgt tgatgaaagc gaaccgttcg cacatgaaaa actgtccccg actctggcaa
     1141 tgtaccgcgc taaagatttc gaagacgcgg tagaaaaagc agagaaactg gttgctatgg
     1201 gcggtatcgg tcatacctct tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt
     1261 cttacttcgg tcagaaaatg aaaacggctc gtatcctgat taacacccca gcgtctcagg
     1321 gtggtatcgg tgacctgtat aacttcaaac tcgcaccttc cctgactctg ggttgtggtt
     1381 cttggggtgg taactccatc tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa
     1441 ccgttgctaa gcgagctgaa aacatgttgt ggcacaaact tccgaaatct atctacttcc
     1501 gccgtggctc cctgccaatc gcgctggatg aagtgattac tgatggccac aaacgtgcgc
     1561 tcatcgtgac tgaccgcttc ctgttcaaca atggttatgc tgatcagatc acttccgtac
     1621 tgaaagcagc aggcgttgaa actgaagtct tcttcgaagt agaagcggac ccgaccctga
     1681 gcatcgttcg taaaggtgca gaactggcaa actccttcaa accagacgtg attatcgcgc
     1741 tgggtggtgg ttccccgatg gacgccgcga agatcatgtg ggttatgtac gaacatccgg
     1801 aaactcactt cgaagagctg gcgctgcgct ttatggatat ccgtaaacgt atctacaagt
     1861 tcccgaaaat gggcgtgaaa gcgaaaatga tcgctgtcac caccacttct ggtacaggtt
     1921 ctgaagtcac tccgtttgcg gttgtaactg acgacgctac tggtcagaaa tatccgctgg
     1981 cagactatgc gctgactccg gatatggcga ttgtcgacgc caacctggtt atggacatgc
     2041 cgaagtccct gtgtgctttc ggtggtctgg acgcagtaac tcacgccatg gaagcttatg
     2101 tttctgtact ggcatctgag ttctctgatg gtcaggctct gcaggcactg aaactgctga
     2161 aagaatatct gccagcgtcc taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg
     2221 ttcacagtgc agcgactatc gcgggtatcg cgtttgcgaa cgccttcctg ggtgtatgtc
     2281 actcaatggc gcacaaactg ggttcccagt tccatattcc gcacggtctg gcaaacgccc
     2341 tgctgatttg taacgttatt cgctacaatg cgaacgacaa cccgaccaag cagactgcat
     2401 tcagccagta tgaccgtccg caggctcgcc gtcgttatgc tgaaattgcc gaccacttgg
     2461 gtctgagcgc accgggcgac cgtactgctg ctaagatcga gaaactgctg gcatggctgg
     2521 aaacgctgaa ggctgaactg ggtattccga aatctatccg tgaagctggc gttcaggaag
     2581 cagacttcct ggcgaacgtg gataaactgt ctgaagatgc attcgatgac cagtgcaccg
     2641 gcgctaaccc gcgttacccg ctgatctccg agctgaaaca gattctgctg gatacctact
     2701 acggtcgtga ttatgtagaa ggtgaaactg cagcgaagaa agaagctgct ccggctaaag
     2761 ctgagaaaaa agcgaaaaaa tccgcttaat cagtagcgct gtctggcaac ataaacggcc
     2821 ccttctgggc aatgccgatc agttaaggat tagttgaccg atccttaaac tgaggcacta
     2881 taacggcttc cacaacaggg agccgttttc ttatgccact tcttaatgat ctgctcgatt
     2941 tcagtgacca tccgcttatg cctccgccct ctgcacaact atttgcagaa caccttccca
     3001 ccgagtggat acaacactgc ctgacgcttt ctgctcatgc gaccgttcgc cgccgtcgtt
     3061 taccggggga catggttatc tggatggtgg tgcaatgagc caattaccga tgttgttcgc
     3121 cgtctgaacc tgagcgcgga tggcgaagcg gggatgaacc tgctggcccg cagcgctgtc
     3181 acccaggcgc gtcagcgcgt gggggccgcc ccagtggaat ggctcttccg ccagaccgca
     3241 cagacagggg cgcggaacgt tacctgaagg atgactggca cggcctgcaa ctttttgcca
     3301 ttgatggcgc acagttcagg acacctgata aacccgagct gcgtgaatat tatgggtctg
     3361 ccaacacatc cactaagcgg cagaacgcct acccggttat gcgtctggtg gccttaatga
     3421 acctgggaag tcacattctg ctgaatgccg tgaccgcacc ttaccggcag agcgaaactg
     3481 tgctggccca ctccatgctc gccaccatcc cggataactc tattacgttg tttgataaac
     3541 ttttttacag cgaagacctg ctgctgacgc tgaaccagaa gggttgtaac cgccactggc
     3601 tactgcctgc gtggaagaat atcgcatcag aaatgataga gctgggaaac acggcgtcaa
     3661 cagggaccat cccgaaacga ctggagcatc ttcggggtgc tctggaagtg gtgttcataa
     3721 caaaacgccc ccgaccatca aggccgaggt cggttaagat atcaaaaacc cgttatcctg
     3781 tgaaacatag tgccgctcct cttaagtgaa cggcattgcc cttctggggc cgtatttttt
     3841 gtttacccaa agcaactttt ccataaaccg acagcattag ccttcatcat atttgcgacg
     3901 atgtataacg cctaaacaca gggatattgt actttacagg tcacaagtca acgtcggtgc
     3961 ttaagagccc tgtgaggcgt atagcggcgt taaaaaactg ccgagaaggg tatatagccc
     4021 ggaagaagtg cgtaaaacga actgacagga taaaagtgcc ctgctcaccc tgtcagtaaa
     4081 gaaattctta ttaatcgtgg cgatgccttt cctgaatagc cgttaatgag ccgacttgta
     4141 acgcctcttt atagtgttta cggcatacag aaacgtatcg ttcattacca ccaattacca
     4201 cctgctcacc ttcgttataa ggtctgcctg cttgatcaag acgcagcacc atgcttgctt
     4261 tacggccaca aaaacagatg gtttttaatt caaccagttt gtcggaccat gccagtaagt
     4321 attggctgcc aataaataat tcacctcgaa aatcggtacg taaaccataa caaagtacgg
     4381 gtatatcgag ttgatcgaca acctccgata attcatatac ttgttgtctg gttaaaaact
     4441 ggcattcatc aaccagtacg caatgaattg cctgctgttc atgttccgca cgaatctcat
     4501 caaataatga tgaattttgg ttaaataatt ttgcaggcga tgacaaacct atacgcgaac
     4561 tgactttccc ggcaccaaag cgatcatcaa tttctgccgt atatacgaca gtgcgcatgc
     4621 cgcgttcctg gtaattgtat gaagattgca acaatgctgt agacttaccc gcattcattg
     4681 cggaatagta gaaatatagc tgtgccatca gccacaggcc ctcaatgata tgaaataaag
     4741 ttggctggag tttatcataa ttcgtcagtt tttcagctca acagtatgca cagaagtggg
     4801 tatatgcgtt ctcccttacg aagccttgca taatccttct gagctatcat tacaactgcc
     4861 tcgcttgtta taagcgggta aatgactgct ggtaactatt cacaatcttt aacctgttgc
     4921 gcaagtaata gccctctgtt gacctccagg agatagtgca atactaagtc catgctctta
     4981 ttgcgactgt tctacttttc atcattcgct taatagggaa ttctcgtaaa cacaactaat
     5041 acagaagact gaaaggtcgt cagcctacga taatctcccc ataaaatgtg acatgaatca
     5101 ggaagtttta acctcacgtg ctgcgaaatc atcggtgtaa atagggctat atgccgcgtc
     5161 ttttctggct aattttatga aaagatattt attggcggca caaaataaag aacaattttg
     5221 aattccttac attcctggct attgcacaac tgaatttaag gctctattat tacctcaaca
     5281 aaccacccca atataagttt gagattacta caatgagcga agcacttaaa attctgaaca
     5341 acatccgtac tcttcgtgcg caggcaagag aatgtacact tgaaacgctg gaagaaatgc
     5401 tggaaaaatt agaagttgtt gttaacgaac gtcgcgaaga agaaagcgcg gctgctgctg
     5461 aagttgaaga gcgcactcgt aaactgcagc aatatcgcga aatgctgatc gctgacggta
     5521 ttgacccgaa cgaactgctg aatagccttg cttccgttaa atctggcacc aaagctaaac
     5581 gtgctcagcg tccggcaaaa tatagctacg ttgacgaaaa cggcgaaact aaaacctgga
     5641 ctggccaagg ccgtactcca gctgtaatca aaaaagcaat ggatgagcaa ggtaaatccc
     5701 tcgacgattt cctgatcaag caataatctt ttgtagattg cacttgctta aaatcccgcc
     5761 agcggcg
//
LOCUS       DAZFWL020000218         5699 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFWL020000218 DAZFWL020000000
VERSION     DAZFWL020000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.219"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            73..615
                     /gene="fimA"
                     /locus_tag="ACHKNP_RS23635"
                     /old_locus_tag="pgaptmp_004727"
     CDS             73..615
                     /gene="fimA"
                     /locus_tag="ACHKNP_RS23635"
                     /old_locus_tag="pgaptmp_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4706739.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            835..1527
                     /gene="fimC"
                     /locus_tag="ACHKNP_RS23640"
                     /old_locus_tag="pgaptmp_004728"
     CDS             835..1527
                     /gene="fimC"
                     /locus_tag="ACHKNP_RS23640"
                     /old_locus_tag="pgaptmp_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU4706740.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRLIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1558..4167
                     /gene="sfmD"
                     /locus_tag="ACHKNP_RS23645"
                     /old_locus_tag="pgaptmp_004729"
     CDS             1558..4167
                     /gene="sfmD"
                     /locus_tag="ACHKNP_RS23645"
                     /old_locus_tag="pgaptmp_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGU4706741.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERISLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGTVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGRKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4209..5186
                     /gene="fimH"
                     /locus_tag="ACHKNP_RS23650"
                     /old_locus_tag="pgaptmp_004730"
     CDS             4209..5186
                     /gene="fimH"
                     /locus_tag="ACHKNP_RS23650"
                     /old_locus_tag="pgaptmp_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU4706742.1"
                     /translation="MACLCLANISWATVCANSTGVAEDEHYDLSNIFNSTNNQPGQIV
                     VLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLHDYLLGAMSLVD
                     SVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMVEIPRQVMFTV
                     YVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASLFSAAGPGNRP
                     AGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITP
                     NDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
     gene            5197..>5699
                     /gene="sfmF"
                     /locus_tag="ACHKNP_RS23655"
                     /old_locus_tag="pgaptmp_004731"
     CDS             5197..>5699
                     /gene="sfmF"
                     /locus_tag="ACHKNP_RS23655"
                     /old_locus_tag="pgaptmp_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HGU4706743.1"
                     /translation="MRRVLFSCFCGLLWSSIGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFM"
BASE COUNT         1498 a         1235 c         1372 g         1594 t
ORIGIN      
        1 tatcagtgtc ttaaataaag taatcggtta tatacggatg tggagtcgat aaatgagatt
       61 gaaggaatat atatgaaatt aagatttatt tcgtctgcgc tggctgccgc actattcgcc
      121 gctacgggta gttatgctgc cgttgtagat ggcggtacaa ttcactttga aggcgaactg
      181 gtgaatgctg cctgttcagt gaatactgac tcggcagacc aggttgtcac actcggtcaa
      241 tatcgtaccg atattttcaa tgctgttggt aatacctctg cattaattcc attcaccatt
      301 cagttgaacg actgcgatcc tgttgttgcc gctaatgctg ccgttgcatt ttctggtcag
      361 gctgatgcaa tcaatgataa tttattggcc attgcatcca gtaccaatac aacaacagca
      421 acgggtgtcg gtattgaaat acttgataat acatccgcaa ttctcaaacc tgatgggaat
      481 agcttctcaa ccaaccagaa cttgatcccc gggaccaacg ttcttcattt ttctgcacgt
      541 tataaaggca ccggtacaag tgcatcagca gggcaagcaa atgctgacgc gacttttatt
      601 atgagatacg aataatcaaa accacgttgt tttgaattat atatcacgtc ttataacaaa
      661 gtaatgtacc ggttgtctga agcggtatgg tggcaatgta aatcgaaatc atgttcactt
      721 tgtatcatgc cgctttatta aatgaaaagg gaatgatgtg ttgtaagaaa ccaaagcaat
      781 catttcttta tatttcttat ttttgccgtc aggaatacac aaggcgtatt aactatgatg
      841 actaaaataa agttattgat gctcattata ttttatttaa tcatttcggc cagcgcccat
      901 gctgccggag ggatcgcatt aggtgccacg cgtattattt atcccgctga tgctaaacag
      961 actgcggtat ggattagaaa tagccatacc aatgagcgct ttctggtcaa ttcgtggatt
     1021 gaaaacagca gcggtgtaaa agaaaagtca ttcatcatta caccgccact gtttgttagt
     1081 gaacccaaaa gcgaaaatac cttgcgtatt atttacaccg gtccaccgct ggcagcagat
     1141 cgtgagtctc tgttctggat gaatgttaag acgatccctt cggtagataa aaatgcattg
     1201 aacggcagga atgttttgca actggcgatt ttatcgcgca tgaaattatt tctccgtcta
     1261 attcaattac aagaattacc cgcagaagca ccggacacac tcaagttttc gcgatccggt
     1321 aactatatca atgttcataa tccctcacct ttttatgtca ctctggttaa cttacaagtg
     1381 ggcagccaaa agttggggaa tgctatggct gcacccagag ttaattcaca aattccctta
     1441 ccctcaggag tgcagggaaa gctgaaattt cagaccgtta atgattatgg ttcagtaacc
     1501 ccggtcagag aagtgaactt aaactaaccg aatcatctga caatatcaga gctaattatg
     1561 aaaataccca ctactacgga tattccgcag aggtatacct ggtgtctggc cggaatttgt
     1621 tattcatctc ttgccatttt accctccttt ttaagctatg cggaaagtta tttcaacccg
     1681 gcatttttat tagagaatgg cacatccgtt gctgatttat cgcgctttga gagaggtaat
     1741 catcaacctg cgggcgtgta tcgggtagat ctctggcgta atgatgagtt cattggttca
     1801 caggatatcg tatttgaatc gacaacagaa aatacaggtg ataaatcagg tgggttaatg
     1861 ccctgtttta accaggtact ccttgaacga attagcctta atagcagtgc atttcccgag
     1921 ttagcccagc agcaaaacaa taaatgcatc aatttactga aagctgtacc tgatgccaca
     1981 attaactttg attttgcagc gatgcgcctg aacatcacta ttcctcagat agcgttgttg
     2041 agtagcgctc acggttacat tccgcctgaa gagtgggatg aaggtattcc tgctttactc
     2101 ctgaattata atttcaccgg taacagaggt aatggtaacg atagctattt ttttagtgag
     2161 ctcagcggga ttaatattgg cccgtggcgt ttacgcaaca atggttcctg gaactatttt
     2221 cgcggaaatg gatatcattc agaacagtgg aataatattg gcacctgggt acagcgcgcc
     2281 attattccgc tgaaaagtga actggtaatg ggagacggca atacaggaag tgatattttc
     2341 gatggtgttg gatttcgtgg tatacggctt tactcttccg ataatatgta tcctgatagc
     2401 cagcaagggt ttgccccaac agtacgtggg attgcccgta cggcggccca gctaacgatt
     2461 cggcaaaatg gttttattat ctatcaaagc tatgtttccc ccggcgcttt tgaaattaca
     2521 gatttgcacc cgacatcttc aaatggcgat ctggacgtca ccatcgacga gcgcgatggt
     2581 aatcagcaga attacacaat tccgtattca acagtgccaa ttttacaacg cgaagggcgt
     2641 ttcaaatttg acctgacggc gggcgatttt cgtagcggta atagtcagca atcatcgcct
     2701 ttcttttttc agggtacggc actcggcggt ttaccacagg aatttactgc ctacggcggg
     2761 acgcaattat ctgccaatta caccgccttt ttattagggc tggggcgcaa cctcgggaac
     2821 tggggcacag tgtcgctgga tgtgacccat gcgcgcagtc agttagccga cgacagtcgt
     2881 catgaggggg attccattcg cttcctctat gcgaaatcga tgaacacctt cggcaccaat
     2941 tttcagttaa tgggttaccg ctattcgaca caaggttttt atacccttga tgatgttgcg
     3001 tatcgtcgaa tggaggggta cgaatatgat tacgattacg acggtgagca tcgcgatgaa
     3061 ccgataatcg tgaattacca caatttacgc tttagccgta aagaccgttt gcagttaaat
     3121 atttcacaat cacttaatga ctttggctcg ctttatatct ctggtaccca tcaaaaatac
     3181 tggaatactt cggattcaga tacctggtat caggtggggt ataccagcag ctgggttggc
     3241 atcagttatt cactctcatt ttcgtggaat gaatctgtag ggatccccga taacgaacgt
     3301 attgtcggac ttaatgtttc agtgcctttc aatgttctga ccaaacgtcg ctacacccgg
     3361 gaaaatgcgc tcgaccgcgc ttatgcctcc tttaacgcca accgtaacag caacgggcaa
     3421 aatagctggc tggcaggtgt aggtgggacc ttactggaag gccacaacct gagttatcac
     3481 gtaagccagg gtgatacctc gaataatggg tatacgggca gcgccacggc aaactggcag
     3541 gccgcttacg gtacgctggg ggtcgggtat aactacgacc gcgatcaaca tgacgttaac
     3601 tggcagctgt ctggcggtgt ggtcgggcat gaaaatggca taacgctgag ccagccttta
     3661 ggggatacca atgttttgat taaagcgcct ggcgcaggcg gtgtacgcat tgaaaatcaa
     3721 actggcattt taaccgactg gcgcggctat gcggtgatgc cgtatgccac ggtttatcgg
     3781 tataaccgta tcgcgcttga taccaatacg atggggaatt ccatcgatgt tgaaaaaaat
     3841 attagcagcg ttgtgccgac gcaaggcgcg ttggttcgtg ccaattttga tacccgcata
     3901 ggcgtgcggg cgctcattac cgttacccag ggcagaaaac cggtgccgtt tggatcactg
     3961 gtacgggaaa acagtaccgg aataaccagt atggtgggtg atgacgggca agtttattta
     4021 agtggtgcgc cattgtctgg tgaattactg gttcagtggg gagacggcgc gaactcacgc
     4081 tgcattgcgc actatgtatt gccgaagcaa agcttacagc aagccgtcac tgttatttcg
     4141 gcagtttgca cacatcctgg ctcataaagg aaattatcaa taagataatc tgcagattat
     4201 tattggcgat ggcatgtttg tgtctggcaa acatatcctg ggctactgtt tgtgcaaata
     4261 gtactggcgt agcagaagat gaacactatg atctctcaaa tatctttaat agcaccaata
     4321 accagccagg gcagattgtt gttttaccgg aaaaatccgg ctgggtaggt gtctcagcaa
     4381 tttgtccacc cggtacgctg gtgaattata cataccgtag ttatgtcacc aactttattg
     4441 ttcaggaaac tatcgataat tataaatata tgcaattaca tgattatcta ttaggtgcga
     4501 tgagtctggt tgatagtgtg atggatattc agttcccccc gcaaaattat attcggatgg
     4561 gaacagatcc taacgtttcg caaaaccttc cattcggggt gatggattct cgtttaatat
     4621 ttcgtttaaa ggttattcgt ccctttatta acatggtgga gatccccaga caggtgatgt
     4681 ttaccgtgta tgtgacatca acgccttacg atccgttggt tacacctgtt tataccatta
     4741 gttttggtgg ccgggttgaa gtgccgcaaa actgcgaatt aaatgccggg cagattgttg
     4801 aatttgattt tggtgatatc ggcgcatcgt tatttagtgc ggcagggccg gggaatcgac
     4861 cagctggcgt catgccgcaa accaagagca ttgcggtcaa atgtacgaat gttgctgcgc
     4921 aggcttattt aacaatgcgt ctggaagcca gtgccgtttc tggtcaggcg atggtgtcgg
     4981 acaatcagga tttaggtttt attgtcgccg atcagaacga tacgccgatt acgcctaacg
     5041 atctcaatag cgttattcct ttccgtctgg atgcagctgc ggcagccaat gtcacacttc
     5101 gcgcctggcc tatcagtatt accggtcaaa aaccgaccga ggggccgttt agcgcgctgg
     5161 ggtatttacg cgtcgattat caatgaggta cggagaatga gaagagtact ctttagctgt
     5221 ttctgcgggt tactgtggag ttccattgga tgggcagctg accctttagg aacgattaat
     5281 atcaatttgc acggtaacgt tgttgatttc tcctgtaccg taaacacagc ggatattgat
     5341 aagacggtag atttaggcag atggcctacg acacaattac tgaacgctgg cgataccacg
     5401 gcactcgtcc cttttagcct gcggctggag ggatgtcctc cgggttcagt tgcgatttta
     5461 tttacgggaa cgccggcatc cgataccaac ctgctggctc tggatgatcc cgcaatggca
     5521 caaaccgtcg ccatcgaatt acgtaatagc gatcgctccc ggctcgcact gggggaggcg
     5581 agcccgactg aggaagtaga tgcaaatggc aatgtcacac taaacttttt tgccaattat
     5641 cgagcgttag ccagcggtgt tcggcctggt gtggcgaaag cggatgcgat atttatgat
//
LOCUS       DAZFWL020000219         5481 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFWL020000219 DAZFWL020000000
VERSION     DAZFWL020000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.220"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..350)
                     /locus_tag="ACHKNP_RS23660"
                     /old_locus_tag="pgaptmp_004732"
     CDS             complement(<1..350)
                     /locus_tag="ACHKNP_RS23660"
                     /old_locus_tag="pgaptmp_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4706744.1"
                     /translation="MQGATGLELYEAYNNGYPTTYGNIIHLKGMTAVGEGELLIGWSG
                     ISGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPEEFYPVGAPIPWPSDTVPSGYALM
                     QGQTFDKSAYPKLAV"
     gene            complement(915..1628)
                     /locus_tag="ACHKNP_RS23665"
                     /old_locus_tag="pgaptmp_004733"
                     /pseudo
     CDS             complement(915..1628)
                     /locus_tag="ACHKNP_RS23665"
                     /old_locus_tag="pgaptmp_004733"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(1631..2161)
                     /locus_tag="ACHKNP_RS23670"
                     /old_locus_tag="pgaptmp_004734"
     CDS             complement(1631..2161)
                     /locus_tag="ACHKNP_RS23670"
                     /old_locus_tag="pgaptmp_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HGU4706745.1"
                     /translation="MSNSLLPPSASSFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATHDGEVITIYPDD"
     gene            complement(2154..3050)
                     /locus_tag="ACHKNP_RS23675"
                     /old_locus_tag="pgaptmp_004735"
     CDS             complement(2154..3050)
                     /locus_tag="ACHKNP_RS23675"
                     /old_locus_tag="pgaptmp_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGU4706746.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTTAFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLT
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            complement(3054..3404)
                     /locus_tag="ACHKNP_RS23680"
                     /old_locus_tag="pgaptmp_004736"
     CDS             complement(3054..3404)
                     /locus_tag="ACHKNP_RS23680"
                     /old_locus_tag="pgaptmp_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HGU4706747.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            complement(3401..3967)
                     /locus_tag="ACHKNP_RS23685"
                     /old_locus_tag="pgaptmp_004737"
     CDS             complement(3401..3967)
                     /locus_tag="ACHKNP_RS23685"
                     /old_locus_tag="pgaptmp_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU4706748.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWCVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDANPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(3979..4614)
                     /locus_tag="ACHKNP_RS23690"
                     /old_locus_tag="pgaptmp_004738"
     CDS             complement(3979..4614)
                     /locus_tag="ACHKNP_RS23690"
                     /old_locus_tag="pgaptmp_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HGU4706749.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(4607..5074)
                     /locus_tag="ACHKNP_RS23695"
                     /old_locus_tag="pgaptmp_004739"
     CDS             complement(4607..5074)
                     /locus_tag="ACHKNP_RS23695"
                     /old_locus_tag="pgaptmp_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4706750.1"
                     /translation="MLKTNSLRKGMIHGCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGAPQAEHLQEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(5098..>5481)
                     /locus_tag="ACHKNP_RS23700"
                     /old_locus_tag="pgaptmp_004740"
     CDS             complement(5098..>5481)
                     /locus_tag="ACHKNP_RS23700"
                     /old_locus_tag="pgaptmp_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4706751.1"
                     /translation="LGIYLYTDVALPDVVAMTGTGNPFLMSFFTQTTDGKLNLMHHKK
                     AGNTKLGEFGNYSNDWQTLELVFTASSATVTPKLNGVAGPAFQVIKDSLTLGLNALTL
                     TDITKNATYGVEIESLVLEINSPAA"
BASE COUNT         1095 a         1573 c         1448 g         1365 t
ORIGIN      
        1 actgcaagtt tcgggtatgc agatttgtca aaagtctgcc cctgcatcag ggcataacca
       61 gacggaacgg tatctgatgg ccacggaatc ggtgcaccga ctggataaaa ctcttcagga
      121 ggatgagccg aggtgtaaag ctgcgcccac ggcgaccagt ttgcgtcggt cgtatcccgt
      181 cgtgaacgaa taaatgccgg agcatgagca ccgcttatac cactccagcc gatgagtaat
      241 tcgccttcgc caacggctgt catccctttc aggtgaatga tatttccata cgttgttgga
      301 tatccgttgt tatacgcctc gtataactcc agacctgtgg ccccctgcat attatctgtc
      361 agggcggtca gccgaccttt tgaagccaga ttaactgacg atactgctgt cccatctgac
      421 ggtaacgccc cgatctctga tgctgttggc ttattcctgg agttatagtc ccttcgccag
      481 ccaggagcat aagcatcacc atgattaata taagtaaatt gagcattagg gatgccttca
      541 ccgctggatg tactcggtgt ggtaatgcgt atggtcattg cgccgcgggt gccaataact
      601 tccacaacag cacctgcaag acaaatactt ccgcaacctg tatctgtaat gaccttatta
      661 tttgcataag cccatgagcc tttgcacatc cagtaaggat ggttaaatgc cccctgactc
      721 tctagccaaa aaatcaactg cgcagttgtc catgcttcac tatctccacc aatattcagc
      781 tctgcactat atgcacggca ggcaccgata tttttcgtaa aggtgtcttt atcagagata
      841 tcctcaccat tcttagcttt ctgcagacgt ttttcagcat tgtcataggc agcttttact
      901 gcatttgatg ttgccgccag cgtttcagat gtactgttgg ttgcactact aagctggaca
      961 aggccttttc gcgctgttgt ggcatcctgc gctgtatatt ttccgttagc aagatcatac
     1021 gcgaccttaa ccgctttcgg cgttgcagcc agcgtttcag aatcgctgtt agtggcgcta
     1081 ctgagttgaa caaagccttt tgcggtcagc gaggcatccg ggtgacatcg tgactgttca
     1141 tgctctttca gtttgttatc cacgtaatcc actgtggcca tcaccatggt gttatccacg
     1201 gtaagcgcca cagtggcagt gctggatacg gtcagaatgg tgcgaaatgt ttgtgcacgt
     1261 ccggaccctt cggcaacggc tggcttgtaa ctttcggcag tattgcccac cgcgattaaa
     1321 tcgccgtgct catcaaacac accaatttcc cggatccaga atccgcccgt ttctggagga
     1381 ataaccagct ccgcaataat gcggttctga tgtgttgcgt ccaggatgac gcgattaaca
     1441 gtgtgtcgcc acacctcatg caccagacgg gtctgcttac tgtctggtgt gggcaacgcg
     1501 ccgccaccgt cgcccacggc catatgagtc aggcggacag gcttaccatc tggcgcggct
     1561 gcctgagcta attttttggc acctgtatcg gtgataacgg ttttaaattt tcgtgttgtg
     1621 gtactcatgc ttaatcgtcc ggataaatgg taataacttc accgtcatga gttgccgccg
     1681 ctgcaaaaat atcccccgga atttcctgaa tgatattcag ccctgtcatg tggcggctga
     1741 ccggacgggc atcagcaatc aaccgctcca tttccagata catttcctcc gtcacgccac
     1801 tgtccagtgt gccgacttca acggtaaatg ttcccggttc tccgccgaac tcccaccact
     1861 cagacacgcg aatgaggtat cccagcggct caatggccct gcgcagtgcg ctgatggtcc
     1921 ctttgtgtcg gtgtatcagc catgcatcac gaatcacctg tcgttttgtc tcttccggcc
     1981 agttgcgatc ccagcggtca acggaaaatg cccaggcgag ataaggcagc aggtgcaccg
     2041 ggcaggtgtc cggcgaccac agcgtgttga ggtctaccgg gatgtctgta atgcgcgttc
     2101 cgacagcttc ggcacaacgc atgaaactgc tggctgatgg tggtaacagt gaattactca
     2161 ttgcgcccac cttcgctgat ggtgaacgac tcacagcgcg ccgcctgtat gtcgctgatg
     2221 gccatattct gtgtgggttc gattatctcc acgcgttgca caccgtgcac atgcagtgcg
     2281 gcagcaatgg cggacaacgc cacgtcctga ccgataagcc cctgctcagc cagccacttc
     2341 ctgaacgacg attcagccgc cgccagaata ggttcggatt ccgggccggg gtaaaagtac
     2401 agttttgcat tcagccgcca tgtcacgatt ctggcgctct gtaccgtcag gcggtcggcc
     2461 accgggcggg tatcctctgc attcagaacg gcgcgaacgg tattaagcaa cgcctccgtt
     2521 gctgtgccgt cgccttcagt ggacaggatg gaaaccgtca catttgccgg agacggactg
     2581 atagcccgcg catcacgcac cagaccgctg gcgctgcggg caaaatactc gtatgcacct
     2641 gacgggccag caacactcag gccatcgtac gcccgctgcg cccgcagtct cagcgaggtg
     2701 tcactttcca tcactgcgtc ggtggtatcc gttgccggag tgatggtcag gcgctttgtg
     2761 ttcatattgc ccgcgaggtt gtccaggtct gtcccggcgc tgtggcttaa catgcaggcg
     2821 cgtgccccct cattgacccg ctggcgtaac agcatttcac gaaacgctgt tgtctgggcg
     2881 ataacgttca ggggttccga ttccagctcc agcgcggcgg agacggcttc acgctgttcg
     2941 gcaggatagg acgcaatcat catggccttt gtgtcagcca gaattgcctc aaagtcaggc
     3001 tccgcgatga tggcgggttc cggtaactgg gaaaggtcaa cggcaggcat gatttactcc
     3061 ctcagcgtga tggttaattc aacattctgc atggtctgca tgacagtgcc cgacagcgtc
     3121 accccggcgc ggcctcctgc cttccagaca acgtcgatgg cgtccagggc aatgcggggt
     3181 tcccatcgtg tcagcgcaat cacggcagca ctcatgcatt gcagacgagt ggtgttattc
     3241 atgggttcgt caattaaatc gggcacaagg ctgccatatt cccgtcgcat aacccggctt
     3301 gccagcgggg tggtcaggat gtccctgact gactgtttca gatgctccat atcgttcagg
     3361 tttcccgttc cgtccgggtt cattcctgtg tagcgggttg tcactgcggg cctcctgtcg
     3421 aatcgctgcc acctttaacg ccaccgtgtt tatgcgtatg cactgtgatg ccgtttgagg
     3481 tgaaattgcc gccgctgtgc gtgatattgc cgctcatctt tccccctttt gtgacgtcaa
     3541 gcgtcgccgt tctcagaagg tctgtgcatt ccacgacggg cgtgtccagt gtcacactga
     3601 cggatgcctg cagggtggcc gttttcatgc cgctggcgct cagtgcgcct gcgtccgcgt
     3661 cgtagcggaa caccgcgcca tccggcgcgc tgaccacgat ttctttcagg cttttgccgg
     3721 gggccggatt ggcatcactc cacaggctgc caattatcat ggcggtttcc gggttgccgc
     3781 caatgcaggc aattaccacc tgttcgccgg gtgatggcgg cagccacaca ttgaaggctc
     3841 ccgcgcgcgt ggtgttccag cgcaaccagc ctgtttccag ttcgccgctg cgaacgcgca
     3901 cgcaccagga cttctcatca acttcagaga tgatcccagt gcggatgatg ttgctcagca
     3961 gtcgcatgag ttctgcgctc accgtacagc ctccgcaatc cggcccagca ccgtgttata
     4021 aatcaggcgt tcatctgcct gactgatgcc caacagctca cgtaccgggt aatcggtgaa
     4081 aatgcccggc gcaacctgat cgcgctcacc gaactgatga acgcgggcaa tacgtgcggc
     4141 cacgccgctg taacccaccg tcacaccgga agcatctgca cgggctttca ggtagcgggc
     4201 ggtgcgcagt tttacgaaca tggggacgcg ctttgtgctg tcctggttga tgcgccgggt
     4261 gcgtatttcc agaaagcggt cgatgtcatc ccggtaaaac gtgcggatat tgtttttatc
     4321 ctcatcccac ccggtaatgg ttcgcccgta tttccccgtg tcgtgatgcc agtttttcag
     4381 cgtgcgtgct tcgttattcc agataaagcg aatgcgctcc tgtatccggg ttacgcggcg
     4441 tctgcgtggt gtccacgcgg tcccgtccgg cgctttctgt gaccggatac gcgcctgctg
     4501 ggcgcggcgt aaatcctgtg ccagctttct ggcgatgtta ttgatggcct gctgattcag
     4561 gctgtcgcgg atagcctcaa aggtttcatc cacgcgggtg aatgccttat ccatcgcttt
     4621 caccccacgt cacatcctgg aatacatgcg accagtcgcc ttcggaagat ggcagacggg
     4681 gttttggctc ctgcaggtgt tctgcctgcg gtgccccctg actgctgcgc gtgatgcgaa
     4741 cgcgttcccg cagagggagc gtaaacagga gatcggcgct gtcatcgtca ttgataacgg
     4801 cggagaattt gatgtcctga ttacgctccg gattaagcaa caattgcggc tgattttcgg
     4861 ataaccacgc cagtagcggc agcgtgaggt cgtccagctc cccggcgtaa tccatgacaa
     4921 acatcaccat ctgatagcgg taaacaaacg agggcgtttc tccggtcgtt tcaatgttgc
     4981 cgctctccac gaaaatggtg aatttttccg ggttggcctg acaccagcga cacccatgaa
     5041 tcatgccctt ccggaggctg tttgtcttaa gcatctgttt tgctctcctg ttcttcctta
     5101 cgctgcagga ctatttattt ccagcaccag actttctatc tcaacgccat acgttgcatt
     5161 tttggtaata tccgtcagcg tcagtgcatt cagccccagt gtcagactgt cttttatgac
     5221 ctggaatgcc gggccagcca ctccattcag tttcggagta accgtggcac tgctggcggt
     5281 gaacaccagc tccagcgtct gccagtcgtt actgtaattc ccgaactcgc ccaactttgt
     5341 gtttcctgct ttcttgtgat gcatcagatt cagtttgccg tctgtggtct gggtgaagaa
     5401 cgacatcagg aacgggttac cagtcccggt catcgccacg acgtcaggta acgctacatc
     5461 ggtatacaga taaattccca g
//
LOCUS       DAZFWL020000220         5467 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFWL020000220 DAZFWL020000000
VERSION     DAZFWL020000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.221"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(143..391)
                     /locus_tag="ACHKNP_RS23705"
                     /old_locus_tag="pgaptmp_004741"
     CDS             complement(143..391)
                     /locus_tag="ACHKNP_RS23705"
                     /old_locus_tag="pgaptmp_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HGU4706752.1"
                     /translation="MGAYLGSKVKGEDPTVPMVGNAIGTVLGNKAGEKFTKEMLSKGY
                     SPVTSDVIGTVSGGTMTIGTAIDFGIEESQKKGDSNEK"
     gene            755..949
                     /locus_tag="ACHKNP_RS23710"
                     /old_locus_tag="pgaptmp_004742"
     CDS             755..949
                     /locus_tag="ACHKNP_RS23710"
                     /old_locus_tag="pgaptmp_004742"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-zinc-cadmium resistance protein"
                     /protein_id="HGU4706753.1"
                     /translation="MVFVATQHGQKVEFTPQPVKLGQSSGDSQWIEVVNGLNQGQKYA
                     AQGSFLLKSEMEKGEASHEH"
     gene            939..1775
                     /locus_tag="ACHKNP_RS23715"
                     /old_locus_tag="pgaptmp_004743"
     CDS             939..1775
                     /locus_tag="ACHKNP_RS23715"
                     /old_locus_tag="pgaptmp_004743"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HGU4706754.1"
                     /translation="MSTKSPHIPDHESVKPEGLFDRLIQFSIHNAIWVMLFVAAWIAI
                     GIYSYQKLPIDAVPDITNTQVQINTSANGFTALEMEQRITYPIENAMSGMPKMEQTRS
                     ISRYGLSQVTIIFEDGTDIYWARQLINQRLQEAMGEMPEDVQPNMSPISTGLGEIYQW
                     VIKAEPNAKKPDGTPYSAMDLREIQDWIVRPQLQRVKGVAEINSIGGFNKTYVVAPDL
                     NRLQQLQIPLSDLQTALTENNENRGAGYIEKNGQQLTVRVPHSIVNKTFLSFIFNGLF
                     SC"
     gene            2016..2831
                     /gene="sul2"
                     /locus_tag="ACHKNP_RS23720"
                     /old_locus_tag="pgaptmp_004744"
     CDS             2016..2831
                     /gene="sul2"
                     /locus_tag="ACHKNP_RS23720"
                     /old_locus_tag="pgaptmp_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HGU4706755.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2918..3220
                     /locus_tag="ACHKNP_RS23725"
                     /old_locus_tag="pgaptmp_004745"
     CDS             2918..3220
                     /locus_tag="ACHKNP_RS23725"
                     /old_locus_tag="pgaptmp_004745"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706756.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(3604..4440)
                     /locus_tag="ACHKNP_RS23730"
                     /old_locus_tag="pgaptmp_004746"
     CDS             complement(3604..4440)
                     /locus_tag="ACHKNP_RS23730"
                     /old_locus_tag="pgaptmp_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HGU4706757.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(4440..5243)
                     /locus_tag="ACHKNP_RS23735"
                     /old_locus_tag="pgaptmp_004747"
                     /note="aph(3'')-Ib"
     CDS             complement(4440..5243)
                     /locus_tag="ACHKNP_RS23735"
                     /old_locus_tag="pgaptmp_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HGU4706758.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1331 a         1464 c         1362 g         1310 t
ORIGIN      
        1 atcatcctta gaaaaagata tatgactacc ggctttaaag aaactgacca cttgtccaaa
       61 aagaatccca gaaaagctaa gtatagatat tagcaatata caccttgaaa aaagatagaa
      121 taacagccat aagtcaggag atttattttt cattactatc tcctttcttc tgagattctt
      181 caatgccgaa gtcgatagct gtacctatag tcatagtccc cccagagaca gttccaataa
      241 catcagaagt tactggcgag tatcccttcg atagcatttc tttggtgaat ttctctccgg
      301 ctttgttacc cagaacagta cctattgcat tccccaccat tggcactgta gggtcttcgc
      361 ctttaacttt acttcctaag taagctccca ttgtgttaat aaatgtgctg aaaatgacac
      421 ttttaccctg tgtaagccca ccttcacctg cggcaataag agtatccgta gcactatatt
      481 tctcacccgg cttaagggtt aaatactgct taatgccatc tgttacacct cctaaaaggc
      541 tacccccaat aacagcaacg gtacccgcgg ctgggactat aagttcgcca ccaagaacag
      601 aagcacctgc tccccatgca atcagtaaac ctctgacagg atcctgtcct tccggaagat
      661 tacctgttgc aagtttatca gaagcatact taattgtgaa agtaccgcat tacgggtact
      721 taagtccgca gttcagaatg tcgatggaaa agatgtggtt tttgtcgcca ctcagcatgg
      781 acaaaaagtt gaattcactc cgcagccggt gaaattaggt caatcgtctg gggatagcca
      841 gtggattgaa gtggtaaatg ggcttaatca gggacaaaaa tatgccgctc aaggcagttt
      901 cttactgaaa tctgagatgg aaaaaggaga ggcttcacat gagcactaaa agccctcaca
      961 ttccagacca cgaatcggta aaacctgaag ggctgtttga tcggctgatc cagttttcta
     1021 ttcataacgc aatctgggtc atgctgtttg ttgcagcctg gattgctatt ggtatttata
     1081 gttatcaaaa gttaccgatt gatgctgtac cggatattac caatactcag gtgcagatta
     1141 acacttcagc caatggcttt acggctttgg aaatggaaca gcggattacc tatccaattg
     1201 agaatgccat gtcaggcatg ccgaaaatgg aacaaacccg ttcgatttca cgctacggct
     1261 tatcgcaagt gaccatcatt tttgaagatg gcacggatat ttactgggcc agacagctta
     1321 tcaaccagcg cttgcaagaa gcaatgggag aaatgcctga agatgtgcaa cctaacatgt
     1381 cgcctatttc gacaggttta ggtgaaatct atcaatgggt gattaaagcc gaaccaaatg
     1441 ctaaaaagcc ggatggaacc ccttattcgg cgatggattt acgtgaaatt caggattgga
     1501 ttgtaagacc gcaattacag cgcgtaaaag gcgttgcgga gatcaacagt atcggtggct
     1561 ttaataaaac ctatgtggtt gcccctgatc tgaaccgatt gcaacaattg caaattcctt
     1621 tgtctgattt acaaactgcc ttaacagaga ataatgaaaa ccgtggtgct ggctatattg
     1681 agaaaaatgg acaacagcta acggttcgtg ttccccactc tattgtaaac aagacatttt
     1741 tatcttttat attcaatggc ttattttcct gctaattggt aataccatga aaaataccat
     1801 gctcagaaaa ggcttaacaa tattttgaaa aattgcctac tgagcgctgc cgcacagctc
     1861 cataggccgc tttcctggct ttgcttccag atgtatgcta ttctgctcct gcagctaatg
     1921 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgatca tccgtatgag
     1981 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
     2041 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
     2101 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
     2161 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     2221 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     2281 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     2341 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     2401 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     2461 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
     2521 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
     2581 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
     2641 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
     2701 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
     2761 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     2821 gaattcgtta actgcacatt cgggatattt ctctatattc gcggttcagc aggcatgtcc
     2881 cctttgaggg cgacccgacg acaggataat cgaccttatg gtgcgcaaat atttcggcac
     2941 agacggtatt cgtggcaaag ccaacgaagg cgcgatgacg gcggaaaccg ccttgcgcgt
     3001 cggcatggcg gctggccgtg tctttcgtcg cggtgaccac cgccatcgtg tcgtgatcgg
     3061 caaggatacg cgcctgtcgg gctatatgct tgaacccgcg ctcacagccg gtttcacctc
     3121 gatgggcatg gacgtattcc tttttggccc gctgccgaca acgtatagga agaataaacg
     3181 cccttttcac ccaagtccaa cagctttgga ccgcagttga ctctttcgac acccctgcga
     3241 tgcaacccaa tccggctgac ggggagccag caacgctgaa aatttaccct cctctttccc
     3301 actagcggct ccttttccga caaccagcac ggcggatccc tgccgcggcg ctgtgaacgc
     3361 agcattttga ttggtatcgt tggccttcag gctcgtcagt caaacagacc caggagcagc
     3421 tcagccggtg gcgcccggct ttcgggtaac gccctggtcc cgctggtttc ggctttgtgc
     3481 ttcaggtgat caaggatggg gtcgtttgcg ggagagggcg aaatcctacg ctaaggcttt
     3541 ggccaacgat attctccggt aagattgatg tgttcccagg ggataggaga agtcgcttga
     3601 tatctagtat gacgtctgtc gcacctgctt gatcgcggcc gcgatagcta gatcgcgttg
     3661 ctcctcttct ccatccgcgt tccaagctgc ggaaaggcac ccataagcgt acgcctggtc
     3721 gagcaggcga cgcggatcga cgtccagcgc acgagagaat gcgtccgcca tctgtgcaat
     3781 gcgtctagga tcgagacaaa ggtcgtctct gtcagccgga tcgtagaaca tattggcggc
     3841 gccaaagccc acttcaccga ccagaccgac gggatctatc accagccagc cgcgactgga
     3901 gaacatgatg ttttcatgat gcagatcgcc atgtagccca cgcagttccg aggcattgct
     3961 catcatttga tcggctataa tcgccgcgtg gacgtagtca gtttgacaac ctgcgttttg
     4021 atcatcgcgc gcccgctgaa acaaagctgc aaagcgatcc cggatcggga gaagggcaga
     4081 aggcaggggt tcctcagatg cggcatacag cttcgccatt agttccgctg caatttcggt
     4141 cgcctggtag tcgccgtgct cggcaacgat gtgagagagc attcgctccc cggcatattc
     4201 gagcaacatc agattgttct cacgaccgag caaccggact gctcccctcc cattgcgcca
     4261 taccagatag tcggccccgc gcagttcatc agcaatgtct tctataggtt tcaatccctt
     4321 gacgattgca ggagtcccgt ctggcaatga aactttccaa acgaggctgg aaaaggtgtc
     4381 cgcaatgaga acaggttgcg aaacgtgcca atgagcagga aaaacaggcg gcatgaacat
     4441 caaccccaag tcagagggtc caatcgcaga tagaaggcaa ggcgttcgcg gtcgggggct
     4501 tcgatcccca atacattgaa taggacagcg aaggcgcgct ctgcttcatc tggcgctgcc
     4561 cagttctctt cggcgttagc aatcatgagt gccaaatcgg catagcgatc tgctgttccg
     4621 agccgcccaa ggtcgatcag acccgtgcat tgaagagttt tagggtccac catgaagttc
     4681 ggcatgcagg gatcaccatg gcaaacaacc atatcggtgc gctcttggtc gagccgcacc
     4741 ggtagctctc gttcgacacg agccaaaaga tcgagctgcg gcgtactctt gtcctcgtcc
     4801 ggtaagaagt cgggattgac ggcattgcgg gacaccacat caacggcgcg tccgaacatt
     4861 cgcgacagcc tgcgctcaaa cggacattga tcaaccgata ggctgtgaac agcgccaagt
     4921 tgctgcccca ttgacggcca cgctttgagc aaatccgctc cagacagatc agccgccggt
     4981 actcccggaa ttgccgttat caccaagcat gcaccctcct gttcctcctg ccagttgatc
     5041 acctcggggc aagccacacc tcgacctttg agccaaatga ggcggtcacg ctctccagcg
     5101 agctcaccgc ggcgggaagc aggtgcgatt ttcgcgaagg catgcccgtc accacgtcga
     5161 aaaacaaaat caccagattc tccgcctctg acaggcaacc agtcagaatg cgattcacca
     5221 aaaaaaatat tagttcgatt caatggaggt tccttcagtt ttctgatgaa gcgcggaggt
     5281 ggctcaacct gcgaaaagaa acgagttgct acgtaagtcc gagaacatgc tttccatggt
     5341 cttgcaccgg ttgtgttccg gtctatctct cattttaagc ggcttttttc tcaaatgcca
     5401 ccggcgattt ccagccgagt gctgatgaat cccctaatga tttttatcaa aatcattaag
     5461 ttaaggt
//
LOCUS       DAZFWL020000221         5364 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFWL020000221 DAZFWL020000000
VERSION     DAZFWL020000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.222"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            133..1467
                     /gene="garP"
                     /locus_tag="ACHKNP_RS23740"
                     /old_locus_tag="pgaptmp_004748"
     CDS             133..1467
                     /gene="garP"
                     /locus_tag="ACHKNP_RS23740"
                     /old_locus_tag="pgaptmp_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HGU4706759.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1483..2253
                     /gene="garL"
                     /locus_tag="ACHKNP_RS23745"
                     /old_locus_tag="pgaptmp_004749"
     CDS             1483..2253
                     /gene="garL"
                     /locus_tag="ACHKNP_RS23745"
                     /old_locus_tag="pgaptmp_004749"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HGU4706760.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2283..3173
                     /gene="garR"
                     /locus_tag="ACHKNP_RS23750"
                     /old_locus_tag="pgaptmp_004750"
     CDS             2283..3173
                     /gene="garR"
                     /locus_tag="ACHKNP_RS23750"
                     /old_locus_tag="pgaptmp_004750"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU4706761.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3270..4386
                     /gene="garK"
                     /locus_tag="ACHKNP_RS23755"
                     /old_locus_tag="pgaptmp_004751"
                     /pseudo
     CDS             3270..4386
                     /gene="garK"
                     /locus_tag="ACHKNP_RS23755"
                     /old_locus_tag="pgaptmp_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            4419..4795
                     /gene="rnpB"
                     /locus_tag="ACHKNP_RS23760"
                     /old_locus_tag="pgaptmp_004752"
     ncRNA           4419..4795
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKNP_RS23760"
                     /old_locus_tag="pgaptmp_004752"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5190..>5364)
                     /locus_tag="ACHKNP_RS23765"
                     /old_locus_tag="pgaptmp_004753"
     CDS             complement(5190..>5364)
                     /locus_tag="ACHKNP_RS23765"
                     /old_locus_tag="pgaptmp_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706762.1"
                     /translation="DSSSEELLSDNTLEKDENSPDNGFEVGEYNTHEAYNSENQYFTR
                     EDYTYNYDLLNAI"
BASE COUNT         1271 a         1274 c         1498 g         1321 t
ORIGIN      
        1 gcacaatgat tattattctc gcgctgctta tactaattcc cgagttcatt gccgttacgg
       61 cataaatgaa actcataaat aacaccttcc ccataataat aaaaatcagc ataagtaccc
      121 gaggtaaata aaatgattct ggacaccgtt gacgaaaaaa agaaaggcgt gcatacccgc
      181 tatttaatat tactgattat ttttattgtt accgccgtta actacgccga tcgtgcaacg
      241 ctgtctattg ctggtaccga agtggcaaaa gagttgcagt taagtgcggt ttcgatgggt
      301 tacatctttt ctgcttttgg ctgggcctac ttgctgatgc aaatccccgg cggctggctg
      361 cttgataagt ttggctcgaa aaaagtttat acctatagcc tctttttctg gtcgctattc
      421 actttcctgc aaggctttgt tgatatgttc ccgctggcct gggcagggat ctccatgttc
      481 tttatgcgct ttatgctcgg cttctcggaa gcgccatcat tcccggcgaa cgcccgtatt
      541 gtcgccgcct ggttcccgac gaaagaacgt ggtactgcct ccgccatctt taactcggcg
      601 caatatttct cgctggcgct cttttcgcct ctgcttggct ggctgacttt cgcctggggc
      661 tgggagcacg tctttaccgt tatgggggtg attggttttg tgctgacggc gctgtggatc
      721 aaattgattc ataacccgac agatcaccca cgtatgtctg cggaagagct gaagtttatc
      781 tctgaaaatg gcgcggtggt cgatatggac cacaaaaagc cgggcagcgc ggcagcaagc
      841 ggtcccaaac tgcattacat caagcaattg ctctctaacc gcatgatgtt gggcgtattt
      901 ttcggacaat attttatcaa caccatcacc tggttcttcc tcacctggtt cccgatttat
      961 ctggtgcagg aaaaaggcat gtcgattctg aaagtgggtc tggtcgcctc gattccggca
     1021 ctgtgtggtt ttgcgggcgg cgtgctggga ggtgtcttct cggattatct gatcaaacgc
     1081 ggtttatccc tgaccctggc acgtaagcta ccgattgtgc tgggaatgtt gctggcttcc
     1141 accatcatct tatgtaacta caccaacaac accacgctgg tggtcatgct gatggcgctg
     1201 gctttttttg gcaaaggatt tggtgctctg ggctggccgg tgatttctga caccgcgccg
     1261 aaagagattg ttggcctctg tggcggcgtc tttaacgtct ttggcaatgt tgcctccatt
     1321 gtcactccac tggtgattgg ctacctggta agtgaactgc actccttcaa tgcagcactg
     1381 gttttcgtgg gatgttcagc gctgatggcg atggtctgct acctcttcgt agttggcgac
     1441 attaaacgta tggaattgca gaaataagca aaggtataag cgatgaataa cgatgttttc
     1501 ccgaataaat tcaaagccgc actggctgcg aaacaggtac aaattggttg ctggtcagca
     1561 ctctctaacc cgattagcac tgaagttctt ggtttggctg ggtttgactg gctggtgctg
     1621 gatggcgaac atgcgccaaa cgatatctcc acgtttattc cgcagttaat ggccctgaaa
     1681 ggcagcgcca gcgcgccagt agtgcgagtg ccgaccaacg agccggtaat tattaagcgt
     1741 cttctggata tcggtttcta taacttcctg attccttttg tagaaacaaa agaggaagca
     1801 gagcaggcgg tggcatcaac ccgttaccca ccggaaggca ttcgcggcgt ctccgtttct
     1861 caccgcgcca atatgtttgg caccgtggcg gattatttcg ctcagtcgaa caagaacatc
     1921 accattctgg tccagataga aagtcagcag ggcgtagata acgtcgatgc cattgccgct
     1981 accgaaggtg tagacggtat cttcgtcggc cccagcgatc tggctgcggc attaggccat
     2041 ctcggcaatg cctcgcaccc ggatgtacaa aaagcaattc agcacatttt taaccgtgcc
     2101 agtgcgcacg gcaaacccag cggtattctc gcgccggtcg aagccgatgc gcgtcgttat
     2161 ctggaatggg gcgcgacgtt tgtggctgtc ggcagcgatc ttggcgtctt ccgctctgcc
     2221 actcagaaac tggctgatac ctttaaaaaa taaccaccac cgcaacaaga gagatgattg
     2281 atatgactat gaaagttggt tttattggcc tggggattat gggtaaacca atgagtaaaa
     2341 accttctgaa agcaggttac tcgctggtgg ttgctgaccg taacccagaa gctattgctg
     2401 acgtgattgc tgcaggtgca gaaacagcgt ctacggctaa agcgatcgct gaacagtgcg
     2461 acgtcatcat aaccatgctg ccaaactccc ctcatgtgaa agaggtggcg ctgggtgaga
     2521 atggcattat tgaaggcgcg aagccaggta cggtattgat cgatatgagt tctatcgcac
     2581 cgctggcaag ccgtgaaatc agcgaagcgc tgaaagcgaa aggcattgat atgctggatg
     2641 ctccggtgag cggcggtgaa ccgaaagcca tcgacggtac gctgtcagtg atggtgggcg
     2701 gcgacaaggc tattttcgac aaatactatg atttgatgaa agcgatggcg ggttccgtgg
     2761 tgcataccgg ggaaatcggt gcaggtaacg tcaccaaact ggcaaatcag gtcattgtgg
     2821 cgctgaatat tgccgcgatg tcagaagcgc taacgctggc aaccaaagcg ggcgttaacc
     2881 cggacctggt gtatcaggca attcgcggtg gactggcggg cagtaccgtg ctggatgcca
     2941 aagcgccgat ggtgatggac cgcaacttca agccgggctt ccgtattgat ctgcatatta
     3001 aggatctggc aaatgcgctg gatacttccc acggcgtcgg cgcgcaactg ccgctcacag
     3061 ctgcggttat ggagatgatg caggcactgc gagcagatgg tttaggaacg gcggatcata
     3121 gcgccctggc gtgctactac gaaaaactgg cgaaagtcga agttactcgt taatgacgtc
     3181 gtgcccggat ggcgtattgc aatccgggcc tggaatccag gccgaataag agaaacgccc
     3241 tccggcgtca tgtggtaaca ggcataggta tgaaaatcgt aatcgcccca gactcttata
     3301 aagaaagttt atctgccagc gaggttgcgc aggcgataga aaaaggattt cgggaaattt
     3361 ttcctgatgc acagtacgtt tctattccgg ttgccgacgg tggcgaagga acggtggaag
     3421 cgatgattgc agccacccag ggttctgaac gtcacgcctg ggttacaggg ccgctgggcg
     3481 agaaagtgaa tgccagttgg gggatctccg gcgatggcaa aaccgcgttt attgaaatgg
     3541 cggcggccag tgggctggag ctggtacctg cggaaaaacg cgatccactc gtgaccactt
     3601 cacgcggcac aggcgagtta atcctgcagg cgctggagag cggcgcgaca aacattatta
     3661 tcggcattgg cggtagcgct acaaatgatg gcggcgcagg catggtacag gcgctggggg
     3721 cgaaattatg cgacgccaac ggcaatgaaa ttggttttgg cggcggtagt cttaatactc
     3781 tgaatgatat tgatatttcc ggcctcgatc cgcgcttaaa agattgcgtc attcgcgtcg
     3841 cttgtgatgt caccaatccg ctggtgggcg ataacggcgc atcgcgcatc tttggcccac
     3901 aaaagggagc cagtgaagcg atgattgttg agctggacaa taacctctct cactatgccg
     3961 aggtcattaa aaaagcgctg catgttgatg tgaaagatgt ccccggcgct aatggcgttt
     4021 cttggtgcgg aactgaaaag tggtattgaa atcgtcacta cggcgctgaa tctggaggag
     4081 catattcacg attgtacgct ggtgatcacc ggtgaagggc gtattgacag ccagagtatt
     4141 cacggcaagg taccgattgg cgtcgcaaac gtggcgaaga agtaccataa accggtgatt
     4201 ggcattgcgg gtagcctgac cgatgatgtt ggcgttgtac atcagcatgg cattgatgct
     4261 gtcttcagcg tactgaccag cattggtacg ttggacgaag cattccgcgg ggcttatgac
     4321 aatatctgcc gtgcttcacg taatatcgcc gcgacactgg cgattggaat gcgcaacgcg
     4381 gggtgacaag ggcgcgcaaa ccctctatac tgcgcgccga agctgaccag acagtcgccg
     4441 cttcgtcgtc gtcctcttcg ggggagacgg gcggagggga ggaaagtccg ggctccatag
     4501 ggcagggtgc caggtaacgc ctggggggga aacccacgac cagtgcaaca gagagcaaac
     4561 cgccgatggc ccgcgcaagc gggatcaggt aagggtgaaa gggtgcggta agagcgcacc
     4621 gcgcggctgg taacagtccg tggcacggta aactccaccc ggagcaaggc caaatagggg
     4681 ttcataaggt acggcccgta ctgaacccgg gtaggctgct tgagccagtg agcgattgct
     4741 ggcctagatg aatgactgtc cacgacagaa cccggcttat cggtcagttt cacctgattt
     4801 aacgaaaacc cgccagcgta aaccggcggg tttttgcttt tggaggggca gaaagatgaa
     4861 tgactgtcca cgacgctata cccaaaagaa agcggcttat cggtcagttt cacctgatgt
     4921 acgtaataaa ccgttccggc gggtttcaga ttgttgagtg cgctttattc atgccggatg
     4981 cggcgtgaac gccttatcct gcctacaaaa gcacgcaaat tcaatatatt gcagagatgg
     5041 cgtaggcctg ataagcgtag cgcatcaggc atttttgcgg ttgccatcag tttcaaaccc
     5101 gcgtcagtga gtttttgctt ttggtgcaca gggtaagatg aataaatatc tacggtatta
     5161 ataatagact ttgtaagaag ttattaagtc tatattgcat ttaaaaggtc gtaattatac
     5221 gtatagtcct ctctggtaaa atattgattc tctgagttat atgcttcatg tgtgttatat
     5281 tccccgacct caaaaccatt gtccggagaa ttttcgtctt tctcgagggt gttatctgaa
     5341 agtaactctt ctgagctaga gtct
//
LOCUS       DAZFWL020000222         5364 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFWL020000222 DAZFWL020000000
VERSION     DAZFWL020000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.223"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            30..983
                     /gene="ompT"
                     /locus_tag="ACHKNP_RS23770"
                     /old_locus_tag="pgaptmp_004754"
     CDS             30..983
                     /gene="ompT"
                     /locus_tag="ACHKNP_RS23770"
                     /old_locus_tag="pgaptmp_004754"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HGU4706763.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1170..2654
                     /locus_tag="ACHKNP_RS23775"
                     /old_locus_tag="pgaptmp_004755"
     CDS             1170..2654
                     /locus_tag="ACHKNP_RS23775"
                     /old_locus_tag="pgaptmp_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4706764.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKRENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFIAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(2838..3050)
                     /locus_tag="ACHKNP_RS23780"
                     /old_locus_tag="pgaptmp_004756"
                     /pseudo
     CDS             complement(2838..3050)
                     /locus_tag="ACHKNP_RS23780"
                     /old_locus_tag="pgaptmp_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            3200..3868
                     /locus_tag="ACHKNP_RS23785"
                     /old_locus_tag="pgaptmp_004757"
     CDS             3200..3868
                     /locus_tag="ACHKNP_RS23785"
                     /old_locus_tag="pgaptmp_004757"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4706765.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(3813..3950)
                     /locus_tag="ACHKNP_RS23790"
                     /old_locus_tag="pgaptmp_004758"
     CDS             complement(3813..3950)
                     /locus_tag="ACHKNP_RS23790"
                     /old_locus_tag="pgaptmp_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HGU4706766.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(3923..4507)
                     /locus_tag="ACHKNP_RS23795"
                     /old_locus_tag="pgaptmp_004759"
     CDS             complement(3923..4507)
                     /locus_tag="ACHKNP_RS23795"
                     /old_locus_tag="pgaptmp_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4706767.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEEIKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(4507..>5364)
                     /locus_tag="ACHKNP_RS23800"
                     /old_locus_tag="pgaptmp_004760"
     CDS             complement(4507..>5364)
                     /locus_tag="ACHKNP_RS23800"
                     /old_locus_tag="pgaptmp_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4706768.1"
                     /translation="DIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPEGLTDG
                     TVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDM
                     RGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAH
                     THSLSGSTSSAGAHSHVDGRRFNTSTFKDTYQYGSTAVGSNSYQVQGAVGLGIGTMAN
                     TNSAGAHTHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNI
                     AFNYIVRLA"
BASE COUNT         1566 a         1212 c         1130 g         1456 t
ORIGIN      
        1 tcattaaaac gattgaatgg agaactttta tgcgggcgaa acttctggga atagttctga
       61 caacccctat tgcgatcagc tcttttgctt ctaccgagac tatatcgttt actcctgaca
      121 acataaatgc ggacattagt cttggaactc tgagcggaaa aacaaaagag cgcgtttatc
      181 tagctgaaga aggaggccga aaagtcagtc aactcgactg gaaattcaat aacgctgcaa
      241 ttattaaagg tgcaattaat tgggatttga tgccccagat atctattggg gctgcgggct
      301 ggactactct cggcagccga ggtggcaata tggtcgatca ggactggatg gattccagta
      361 accccggaac ctggacagat gaaagtaggc accctgatac acaactcaat tatgccaacg
      421 aatttgatct gaatatcaaa ggctggctcc tcaacgaacc caattaccgc ctgggactca
      481 tggccggata tcaggaaagc cgttatagct ttacagccag aggtggttcc tatatctaca
      541 gttctgagga gggattcaga gatgatatcg gctccttccc gaatggagaa agagcaatcg
      601 gctacaaaca acgttttaaa atgccctaca ttggcttgac tggaagttat cgttatgaag
      661 attttgagct aggtggcaca tttaaataca gcggctgggt ggaagcatct gataacgatg
      721 agcactatga cccaggaaaa agaatcactt atcgcagtaa agtcaaagac caaaattact
      781 attctgttgc agtcaatgca ggttattacg taacacctaa cgcaaaagtt tatgttgaag
      841 gcgcatggaa tcgggttacg aataaaaaag gtaatacttc actttatgat cacaatgata
      901 acacttcaga ctacagcaaa aatggtgcag gcatagaaaa ctataacttc atcactactg
      961 ctggtcttaa gtacaccttt taacgaagtt aaccagattt tctccccggt ggtattgtat
     1021 aaccccgggg aatttgttta cagttacaag taggcaatat ccactaattt tgtaagcatt
     1081 taacctatgt cataacgtgt aatctcttac cgcaacacaa gacctcaata tattcaaatc
     1141 ttatctttat tccgatattc agagacacaa tgcgaaaaat aattactcat ttcaaagttg
     1201 ttttaacgtt acttctacca gtaaccgtat ctgcccagca gatacagtgg caatcctgta
     1261 tggccagtca attcaaccac tggtttggtg aggaaaaacc gtctcctgac ttactatgtg
     1321 gttatttgtc tgttccatta aaatatacag acacaggcgg agatgcttct tatgaaaaaa
     1381 aatcacaagt caaactagcg ttgacaaaat tgccggcaaa aagcaagcat aaaggaagta
     1441 tcctgataat aagtggtggt cccgggttac caggcataaa tccttatatt aactttgact
     1501 ggccagtcac aaatcttcgt gagtcatggg atattattgg atttgatcct cgaggcgtcg
     1561 gacagtccac tccgacaata aactgccggc aatcagatac agagactcag gaaaacataa
     1621 ccgaaaagca acaagtatta aataaaatta atgcctgtat ccataatacc ggagccgaag
     1681 tcattcgcca tatcggctct aacgaggctg tatacgatat tgatcgtatt aggcaagcct
     1741 tgggggataa acaactgaca gccgtggcgt attcgtatgg aactcaaatt gcagccttat
     1801 atgcagaacg ttttccctac aacgtaagat ctatcgttct tgatggagtc gtcgatatcg
     1861 atgacctgga ggacaacttc acatggcaac tcaaacaggc acagagttat caggaaacgt
     1921 ttgatcgctt tgcatcctgg tgtgcgcgta caaaaagttg cccgctttct tcagacagag
     1981 ataaggcaat aactcagttc catgagctat tatcaaaatt acatcacaaa cctttattag
     2041 acagtaagag agaaaatata tcttcagatg aactcatatc attaacaaca gaccttctgc
     2101 tatggcgttc atcatggcca acccttgcaa ctgccatacg ccagttctct caggggattg
     2161 tcagtaatga aattgaaact gcgctcagtg ctccgatagc ctcagaagag tcaagcgatg
     2221 cttcgggggt aatcctctgt gtagatcagg gggatgagca attaacacca gaagagcgaa
     2281 aatcccgaaa agacgctctt gcgaatgcct tcccggctat taactttgac aatggacgtt
     2341 ccgattcacc tgatttttgt gaattatggc caatacatag cgacctgaac aaaactcgcc
     2401 tgaaaaatac tgttctgccc tctggtttac tgtttatagc acacaaatac gacccaacaa
     2461 cgccctggat taatgcccgt aagatggcag agaaattttc cagcccgtta ctaacaataa
     2521 atggtgatgg gcatacatta gctctcaccg gagttaattt atgtgtagat aaagcagttg
     2581 tacatcacct gatcactcca caaaaaatag aaaatatata ctgcccagga aattctgaag
     2641 cagaaataca ataatttcaa aatattccgc tatttgctcc caatgcaaaa catatattgc
     2701 attgggagca tattcatatt tttgttatta ttgtatgaaa tcgtctcatg tacacggtcc
     2761 ttttttcgcc gaaaactgat attactttaa tcaaaagttg acgtgccaga ttcgaagtaa
     2821 atttataaaa tatcaaatca atatacattt tgcaccttcg agatagtaat gactgttact
     2881 gtaacatgag ttacaaacag cttcataccc agagctactg atgtatcaac tcgattcaac
     2941 aatacccgat atttattcca tgcctccagc aacgatcttt cttcctccgt tgcgatttcc
     3001 agatctacag catcctgcag tggcgcaata tactcactga attcctggat gtagaactgt
     3061 gtggtgacgg tcttccagcc attcggctcc tgttgtatcg aagcatacca ggctatttca
     3121 atatcgctat gctgcggcag catttaaccc cttgtaattc atcgccataa ttgatttaat
     3181 tcacaaataa aactataaca tggtgaaatt aatgaaaaaa aacacagatg atggggctaa
     3241 aatttacaca ccacttaccc taaagcttta tgactggtgg gttttgggag tatcaaatcg
     3301 gcttgcatgg ggatgtccta caaaggaaca ccttcttcca cactttctgg aacatttagg
     3361 taacaaccat ctggatattg gtgttggaac tgggttttac cttactcacg tacctgagag
     3421 tagtctgata tctttaatgg atttgaacga agctagcctg aacgcggcat ctacaagggc
     3481 tggggaatca aaaattaaac ataaaattag ccatgatgtt tttgaacctt atcccgcggc
     3541 gttacatggt caatttgatt ccatttccat gttttacctt cttcactgcc tgcctggaaa
     3601 tatatctaca aaaagctgtg taatacgcaa tgcggcgcag gccttaactg acgatggaac
     3661 tctatacgga gccacaattc ttggcgatgg agttgtgcac aatagcttcg gtcaaaaact
     3721 gatgcgcatt tacaatcaga aaggcatctt ttcaaacaca aaagattccg aagaaggctt
     3781 aacacatata ctctcagagc atttcgagaa tgttaaaacc aaggttcaag gtactgtagt
     3841 aatgttttcc gcttcaggga aaaaatagca tccaaccgca gcacgttctt gcttaagacg
     3901 tgctgcggca taatcccaat gattactccc tgacagggtt cgtaggccac tcaatatcag
     3961 gtgcagttga tgtatcaaca cggttcagca acacccgata ctttttccag gcttccagca
     4021 atgaggtttc ttcctccgtt gcgatttcca gatctacagc atcctgaagt ggcgcaatat
     4081 gctcactggc tacctgcatc aggctgtttt ttatttcttc cgcctctcgg atccggaaca
     4141 gtttttctgc ttctgcatct ttcacccagg ctgtgccgtt ccacttctga aactcccctt
     4201 ccggcgataa ccaggtaaca ttttccggta acggaccgag ttcagaaata aataacgcgt
     4261 cccctgacgc tacgtcataa accgttttac cccgatggtc ttcaacgaga tgccacgatg
     4321 cctcatcact gttgaaaaca gccacaaagc cagcaggaat atctggtggt gcaatatcgg
     4381 tactgtttgc tggcagacct gtatgaggcg gaatatatgc atcaccttca ccaataaatt
     4441 cattagttcc ggccagcaga ttataaattt ttatggtccg tgcttgttca ctcattctga
     4501 atgccattat gcaagcctca caatatagtt aaatgcgatg tttttgacgg tgttttccgc
     4561 gttaccagca gcgttaacgg tgatggtgtg tccatgtgag ccaatcgcaa cggagtgcgt
     4621 atgagcacca ataccgacag tatgtgcgtg tgcacctgcg cttgcagcag tgccggacag
     4681 tgagtgggta tgcgcgccag cagagttcgt attcgccatc gtgccaatgc ccagaccaac
     4741 cgccccttgt acctggtatg agttacttcc gacagctgta gaaccatact gataggtgtc
     4801 tttaaaagtg cttgtattaa acctgcgacc atcaacgtgc gagtgtgctc cggccgagct
     4861 tgtagagccg ctcagactgt gcgtatgagc cccggtgtta ttcgtggatt tagtgccgta
     4921 atcaaacgac gatgtggttt tcgtccccaa atccgtactg gatgcgctgg cgctgtgggt
     4981 gtgcgattta atgccgtcct gttcctgaga caatacggca cgaccactgg cgggtttgcc
     5041 cttaatcgtc cagccacgca tatcagggat cacgcctgac ggataagcgg ctgcaagttt
     5101 cgggtatgca gatttgtcaa aagtctgccc ctgcatcagg gcatagccag acggaacggt
     5161 atctgatggc cacgggattg gtgcgccagg cggataaaac tgctctgatg gcgtatagag
     5221 tgaataaact gtaccgtccg ttaacccttc cggcttatta gcagaatatg ctggtgacgt
     5281 atgaatcgtc acgctggcat tactggtata atcccattga atatttacac cagtcgcata
     5341 atttccgatt gcaacgtaaa tatc
//
LOCUS       DAZFWL020000223         5292 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFWL020000223 DAZFWL020000000
VERSION     DAZFWL020000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.224"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..72)
                     /locus_tag="ACHKNP_RS23805"
                     /old_locus_tag="pgaptmp_004761"
     CDS             complement(<1..72)
                     /locus_tag="ACHKNP_RS23805"
                     /old_locus_tag="pgaptmp_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706769.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEE"
     gene            complement(69..635)
                     /gene="yecD"
                     /locus_tag="ACHKNP_RS23810"
                     /old_locus_tag="pgaptmp_004762"
     CDS             complement(69..635)
                     /gene="yecD"
                     /locus_tag="ACHKNP_RS23810"
                     /old_locus_tag="pgaptmp_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU4706770.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            945..2717
                     /gene="aspS"
                     /locus_tag="ACHKNP_RS23815"
                     /old_locus_tag="pgaptmp_004763"
     CDS             945..2717
                     /gene="aspS"
                     /locus_tag="ACHKNP_RS23815"
                     /old_locus_tag="pgaptmp_004763"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGU4706771.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2835..3287
                     /gene="nudB"
                     /locus_tag="ACHKNP_RS23820"
                     /old_locus_tag="pgaptmp_004764"
     CDS             2835..3287
                     /gene="nudB"
                     /locus_tag="ACHKNP_RS23820"
                     /old_locus_tag="pgaptmp_004764"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HGU4706772.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            3316..4056
                     /gene="yebC"
                     /locus_tag="ACHKNP_RS23825"
                     /old_locus_tag="pgaptmp_004765"
     CDS             3316..4056
                     /gene="yebC"
                     /locus_tag="ACHKNP_RS23825"
                     /old_locus_tag="pgaptmp_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4706773.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            4091..4612
                     /gene="ruvC"
                     /locus_tag="ACHKNP_RS23830"
                     /old_locus_tag="pgaptmp_004766"
     CDS             4091..4612
                     /gene="ruvC"
                     /locus_tag="ACHKNP_RS23830"
                     /old_locus_tag="pgaptmp_004766"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HGU4706774.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4614..5216)
                     /gene="yebB"
                     /locus_tag="ACHKNP_RS23835"
                     /old_locus_tag="pgaptmp_004767"
     CDS             complement(4614..5216)
                     /gene="yebB"
                     /locus_tag="ACHKNP_RS23835"
                     /old_locus_tag="pgaptmp_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HGU4706775.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1335 a         1295 c         1435 g         1227 t
ORIGIN      
        1 ctcttcaagg ctggtgatcc ccaaccgcac ccatttagga tgcgaccatt gcggtagacc
       61 gatgtaaatc ataacgcgtg aaggatctct tccacgctac gcacacgggc gatgcgtggg
      121 tagatatggt tgatgctgtt attgtgctgc tcggcgctag cggcactaca ggcgtcttcg
      181 gcaatcacca gattaaagcc aagttcccag gcattgcggg cggtggattc aacaccgata
      241 ttggtcgaga tcccacataa cactattgta tcgatacctc ggcggcgcag ttgcaactcc
      301 agatccgtac cgtaaaacgc accccactga cgtttgatga tttcgatatc actgtcagtt
      361 gcacctaatg cagctggatg ttgccaccag ttttcgggca acactttagc gggggagggg
      421 gcatcaaccg gctgttttaa tgcttcggcg taatcggcag accagccaac gcgcaccaga
      481 aacacgggct gaccgctggc gcgaaatttc gccgccagct tcccggcgcg attaactacc
      541 tcatcggcag tatgtggacc accggcaaaa ggtaagatgc cttcttgtaa atcgatcacc
      601 accagcgcag tggttttagc attaagttca agcataatga ctcctgaaaa aacaaaatcg
      661 tgcctcacac cttaatgcga ttagccgatg gcggaacgac aaattttgtt aatttttatg
      721 agttcgggca acacctttat agcaaactgg cgtagagtct gatttcaggc ccgatataaa
      781 gtggtaacga ggcgttccag cgcgggccag gctcttacgg tgcggcagaa tttccagtat
      841 aatagccgcc ttttttcatt cagttgtgac atacagctaa cgctgcgact tggtcacctg
      901 cgcagcaggg ggcatccgcc ggcagctaag ttaagggata tctcatgcgt acagaatatt
      961 gtggacagct ccgtttgtcc cacgtggggc agcaggtgac tctgtgtggt tgggtcaacc
     1021 gtcgtcgtga tcttggtagc ctgatcttca tcgatatgcg cgaccgcgaa ggtatcgtgc
     1081 aggtattttt cgatccggat cgtgcggacg cgttaaagct ggcctctgaa ctgcgtaatg
     1141 agttctgcat tcaggtcacg ggcaccgtac gtgcgcgtga cgaaaaaaat attaaccgcg
     1201 atatggcgac cggcgaaatc gaagtgctgg cgtcctcgct gactatcatc aaccgcgcag
     1261 atgttctgcc gcttgactct aaccacgtca acaccgaaga agcgcgtctg aaataccgct
     1321 acctcgacct gcgtcgtccg gaaatggctc agcgcctgaa aacccgcgct aaaatcacca
     1381 gcctggtgcg ccgttttatg gatgaccacg gcttcctcga catcgaaact ccgatgctga
     1441 ccaaagccac gccggaaggc gcgcgtgact acctggtgcc ttctcgtgtg cacaaaggta
     1501 aattctacgc actgccgcaa tccccacagt tgttcaaaca gctgctgatg atgtccggtt
     1561 ttgaccgtta ctatcagatc gttaaatgct tccgtgacga agacctgcgc gctgatcgtc
     1621 agcctgaatt tactcagatc gatgtggaaa cttctttcat gaccgcgccg caagtgcgtg
     1681 aagtgatgga agcgctggtg cgtcatttgt ggctggaagt gaagggcgtg gatctgggcg
     1741 atttcccggt aatgaccttt gcggaagcag aacgccgtta tggttctgat aaaccggatc
     1801 tgcgtaaccc gatggaactg accgacgttg ccgatctgct gaaatctgtt gagtttgcag
     1861 tatttgcggg gccggcgaac gatccgaaag gccgtgttgc cgctctacgt gttccgggtg
     1921 gcgcatcgct gacccgtaag cagatcgacg aatacggtaa cttcgttaaa atctacggcg
     1981 cgaaaggtct ggcttacatc aaagttaacg aacgcgcgaa aggtctggaa ggtatcaaca
     2041 gcccggtagc gaagttcctt aatgcagaaa tcatcgaagc catcctggat cgtactgccg
     2101 cgcaagatgg cgatatgatt ttcttcggtg ccgacaacaa gaaaattgtt gccgacgcga
     2161 tgggcgcact gcgcctgaaa gtgggtaaag accttggtct gaccgacgaa agcaaatggg
     2221 caccgctgtg ggttatcgac ttcccgatgt ttgaagacga cggtgaaggc ggcctgacgg
     2281 caatgcacca tccgttcacc tcaccgaaag acatgactgc tgcagaactg aaagctgcac
     2341 cggaaaatgc ggtggcgaac gcttacgata tggtcatcaa tggttacgaa gtgggcggtg
     2401 gttcagtacg tatccataat ggtgatatgc agcagacggt gtttggtatt ctgggtatca
     2461 acgaagagga acagcgcgag aaattcggct tcctgctcga cgccctgaaa tacggtactc
     2521 cgccgcacgc aggtctggca ttcggtcttg accgtctgac catgctgctg actggcaccg
     2581 acaatatccg tgacgttatc gccttcccga aaaccacggc ggcagcgtgt ctgatgactg
     2641 aagcaccgag ctttgctaac ccgactgcac tggctgagct gagcattcag gttgtgaaga
     2701 aggctgagaa taactgatat gactcaaata cacgaaatca ttcgcgttgc atcaaggcgg
     2761 caactgagtg aactcccatg agcatagata actatgtgaa tgggatgagc gaaggcagtc
     2821 aacgaagagg cagcgtgaag gataaagtgt ataagcgtcc cgtttcgatc ttagtggtca
     2881 tctatgcaca agatacgaaa cgggtgctga tgttgcagcg gcgtgacgat cccgatttct
     2941 ggcagtcggt aaccggcagc gtggaagagg gtgaaaccgc gccgcaagct gccatgcgcg
     3001 aagtaaagga agaggtcacc attgatgttg tcgctgaaca actgacctta attgactgtc
     3061 agcgcacggt agagtttgaa attttttcac atttacgtca tcgctatgcg ccgggcgtga
     3121 cgcgtaatac ggaatcatgg ttctgtcttg cgcttccgca cgagcggcag atcgttttca
     3181 ctgaacatct ggcttacaag tggcttgatg cgcctgctgc ggcggcgctc actaagtcct
     3241 ggagcaaccg gcaggcgatt gaacagtttg tcattaacgc tgcctgaaca ggcagggcta
     3301 tctggagata tttttatggc aggtcatagt aaatgggcca acaccagaca tcgtaaagct
     3361 gcgcaggatg caaagcgcgg taaaatcttc actaaaatca ttcgtgagct ggtaaccgcg
     3421 gctaagctgg gcggtggcga tccggacgct aacccgcgtc tgcgtgcagc aattgataaa
     3481 gcactgtcta acaacatgac ccgcgacaca ctgaaccgcg caattgcacg tggtgtgggc
     3541 ggtgatgatg atgcaaacat ggaaaccatc atctacgaag gttacggtcc tggcggcacg
     3601 gcaatcatga ttgaatgtct gtctgacaac cgcaaccgta ccgttgctga agtgcgtcat
     3661 gcattcagca aatgtggcgg taacctcggt actgatggtt ccgtagccta tctgttcagc
     3721 aaaaaaggcg tgatctcctt cgagaaaggc gatgaagaca ccatcatgga agcagctttg
     3781 gaagcaggtg ctgaagacgt tgtgacctat gatgacggcg cgattgatgt ctacaccgca
     3841 tgggaagaaa tgggtaaagt gcgcgacgct ctggaagcgg caggtctgaa agcagacagc
     3901 gcggaagttt ccatgatccc gtccaccaaa gctgatatgg atgcagaaac cgcaccgaaa
     3961 ctgatgcgtc tgatcgatat gctggaagat tgcgacgacg tgcaggaagt ttaccataac
     4021 ggtgaaatct ctgatgaggt tgcagcgact ctctgatgag gcctgctaaa cagcaaaacg
     4081 gagacgcgtg atggctatta ttctcggcat tgatccgggt tcgcgcgtga ccggctatgg
     4141 cgtcatccgc caggtaggca ggcaactgtc ctacctgggt agtggatgca tacgcaccaa
     4201 agtggatgat ttaccgtctc gtctgaagct catctatgcg ggcgtgacgg aaatcatcac
     4261 ccagttccag cctgattatt tcgccattga acaagtcttt atggcgaaga acgctgactc
     4321 agccctgaaa ctgggccagg cgcgcggcgt ggcgattgtg gcggcggtga atcaggaatt
     4381 gccggtattt gaatacgcgg cacgtcaggt aaagcaaacg gtggtaggta ttggtagcgc
     4441 cgagaaaagc caggtgcagc atatggtccg caccttgctg aaactgcccg ctaatccaca
     4501 agcggatgcc gctgatgcgt tagctatcgc gattacccat tgtcacgtca gccagaatgc
     4561 aatgcagatg agcgaatcgc ggctgaacct ggcgagaggg cgactgcgtt aagttatacc
     4621 gcctcggtca gttcaggctg aggcgtttca actccctccg cgtgaatcaa caccaaaccc
     4681 ggatgatgcc acaaactggc tggcgtgacg gttttacgct cccacggaat agaacctaag
     4741 aaccagaatt tccagaaagt gagttttgca ttcggattgc tatttaacag ttgtccaaac
     4801 gtctctattt cacccaccgg aatacatagc gcctctttat aaatatcaaa gacaaatttt
     4861 gaacagaact ggcgggaaga ttcgtattta aaacccgtgt ggtaaagttt gcgtagccgg
     4921 gagggaacct gttcaacaat tcgttgtttc tgttgttctg tcagcccggc gtctaatcgc
     4981 tttatagcat agcgttgatt agcagagcgt ttaataaaac gggatagcgt agtgatggtt
     5041 gagaggggaa cacggctttc tgcaaccaga aagtcttcac cgttatgacc gataatgatc
     5101 ccgacgtgat tactccagca gtttgatgca gctgatattt gaccaaataa ggcagcacct
     5161 atacatgtaa agacgatatc accaatttca tattcagcag ggtaattaat attcacgttg
     5221 tcattccatt gaaatagata tgaatatacg taatagcata aaaaatttat tatttcagga
     5281 gtcgcaatgt at
//
LOCUS       DAZFWL020000224         5233 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFWL020000224 DAZFWL020000000
VERSION     DAZFWL020000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.225"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..358)
                     /locus_tag="ACHKNP_RS23840"
                     /old_locus_tag="pgaptmp_004768"
     CDS             complement(<1..358)
                     /locus_tag="ACHKNP_RS23840"
                     /old_locus_tag="pgaptmp_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGU4706776.1"
                     /translation="MTLTLLTAYNVPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLI"
     gene            complement(625..1290)
                     /locus_tag="ACHKNP_RS23845"
                     /old_locus_tag="pgaptmp_004769"
     CDS             complement(625..1290)
                     /locus_tag="ACHKNP_RS23845"
                     /old_locus_tag="pgaptmp_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HGU4706777.1"
                     /translation="MTILSLENPVILLLENMLEALSTAPDSINNERRRRRYLLNWLES
                     ARQMSAFRGMAEEFTTLRKLLATNTDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(1431..2072)
                     /locus_tag="ACHKNP_RS23850"
                     /old_locus_tag="pgaptmp_004770"
     CDS             complement(1431..2072)
                     /locus_tag="ACHKNP_RS23850"
                     /old_locus_tag="pgaptmp_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HGU4706778.1"
                     /translation="MCLSELQAGLNEDQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNIRELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAPPLPESRIALLLHLVSDDGALT"
     gene            2767..3798
                     /gene="repA"
                     /locus_tag="ACHKNP_RS23855"
                     /old_locus_tag="pgaptmp_004771"
     CDS             2767..3798
                     /gene="repA"
                     /locus_tag="ACHKNP_RS23855"
                     /old_locus_tag="pgaptmp_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HGU4706779.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATSFLVEPERK
                     RTRRRRGEHSTKPKCENPSWYRPARYKELSGQLGHAYNRLVKKAPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAKEISPKD
                     SKGNVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
     gene            complement(4758..>5233)
                     /locus_tag="ACHKNP_RS23860"
                     /old_locus_tag="pgaptmp_004772"
     CDS             complement(4758..>5233)
                     /locus_tag="ACHKNP_RS23860"
                     /old_locus_tag="pgaptmp_004772"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706780.1"
                     /translation="PSSYSHELFDLVEAYRNPTYSYLQNSIINRYIHTVSEHNGKDGV
                     AQMSLPIYAYSTCRFWPKESVAVGDTINLSNYEHIEFSFNSFSKDVCREQNSDIYKIF
                     FKIVALNNSIVVSENMFTQKNFLEHNTFFSVTGIDESLYEKHIIFITLKAIPPQK"
BASE COUNT         1435 a         1254 c         1340 g         1204 t
ORIGIN      
        1 caatcagtct gccggtgcgg tgtacgagag gacatgtcag cagcaggcaa agtggtgcaa
       61 ggagaatgtt cggggcagga gcctgcagca gatgaataat aagaccctgc aggagaatgc
      121 cggatatacc aaaaaaacct gccagcatac agaggacgat cagcaggggg atccgtccga
      181 tgttcagcca gtccagcgcc tctcctgtca gcccctccgg taaatcaggt gtatccatag
      241 ctgaagagag ggagtgtccg cagagcaggg cgataagttc agcgatacca gtaagcagaa
      301 caaaagtcag ggctgccaga tagggtacgt tatacgcggt aagtaacgtc agtgtcattt
      361 ttcctcctgt ggcaggtcag tcaaacaaca ttatggcgtg aagcttccgc ttctttaatt
      421 gtcagaagaa gcatatcgtc agtcatcaga agacaagggg atgtctgcag gaggggatga
      481 taacaactga agcctcacgc cggaatgcgc tttatgacaa ccggagagga cggataataa
      541 aaaatgggac atccacatca gcaaaccgcg atttgcgaaa aaaagtcagc ggagagtggc
      601 tgccgttgtg gctgtcccgg tatttcagtg taacaggtga ttgctgtgat ctatgcccca
      661 ttcggctttc ggtaaacctg aatgccccac atgaagaatg cgtgacagca tgtttctgcc
      721 gctgccgttc aggctgcatt cgcgtccgtg cgcgagtata aaaccttcat cccggaatat
      781 ctcttccact tctgaagatt cgagggatgc cggatgaatt aatgaaaatg ccaccggtct
      841 gagcattctg cccgtggtgg aaaaggtgtc tctggtactg ttcagctgca ggatatgacg
      901 tttacctgtt tgcgcacggg aaagtgtctc actcagcagc tggtcgttcc atgcgcacgt
      961 gccaatctgc catcccagac ttctgcacgc ggaaagagcc gagcggaagc ggtacaggtc
     1021 gtataatgct gcgttattgc cactttccag cagtgatttc agcgtatttg ctaccggtat
     1081 gtccgtattg gtggccagca gttttcgcag agtcgtaaac tcttctgcca ttcctctgaa
     1141 tgcgctcatc tgacgcgcag attcaagcca gttcagcagg taacgccggc gacgccgttc
     1201 gttgttgata ctgtccgggg cggtggagag tgcttcgagc atattctcca gtaacagaat
     1261 gacagggttt tccagggata gaatggtcat aaaggcgtcc tgaaaaaatg cccgacagcc
     1321 gctctccgta caccgtaagg tgtcaccggt gccgggcatt gtggaaacag aaggaaaaaa
     1381 aatcccggcc ataaaggccg ggaaaagtcg tcaaattaag acagcaattt tcatgtcaac
     1441 gctccgtcat cagataccag gtgcaggagc agggcgatcc tcgactccgg cagaggcggt
     1501 gcatcaagcc ggaggaggta ctggtaacgc gcttttgtca gccagagggt ggaggcagca
     1561 tccagctcct ccctggctgc cggattgagc gcaggatccg ggtacacttt catcaaagac
     1621 tgaacgatat cctccctgac ggttttgatt tcaccggcca ttttcacgaa gttcagaaag
     1681 gcactgtgac ggcgaaggtg agtgaagctg tggcgttcgg ggtctgcttt caggaaaaag
     1741 taaccgggaa acacggcgtg tatatgtcga cggtaaccgc agactttatc cgtgcggcgg
     1801 acttttttca gtatcagggg tgtccaggga aggacgtgtt gttcggaaag ccaggaaaag
     1861 agatgttcgc ggtttttgcc ggcagggatg tactgagcca gataccagtt gcgttgatgt
     1921 aattctctga tattcaaaat ccggaacgct ccgcgaaaat cgatccccgc agaggggctt
     1981 cgatgttgtt gtgaacggcg aatacataaa gcatgtgtcg gaaacagcgg ctgatcctcg
     2041 ttcagcccgg cctgcagttc tgacaggcac atgatttacg cactcttgta agccttgtgg
     2101 acggtttcga ggccggcgaa aaccgacaaa acctgcaaac aggattaatg gctggcaaac
     2161 atattacata aggatatatc tgtggcaaga gcgaagataa gtagttaaat agatcgttat
     2221 atttgataaa gcagcatatt acgctgggtt gtgtgaggtg tttggatgat tagatcgtaa
     2281 ttacaagatc gattcatcca aaaaacatga aaaacacagc ataaaagatc gtttgtggaa
     2341 ttgtctattt tttgacacag ttcaaaaaaa cgacgttttt taggtggtta atgagaggcc
     2401 tggtaaatca gagaaatcgt gttggctttt caaagcggtg gaaaaggggt atattgcgga
     2461 tcgttatgca gtggcttttg ggatcgtcag gatccgggaa gtcagaaaat ggcaggatgc
     2521 gccataaggc attcaggacg tatggcagaa acgacggcag tttgccggtg ccggaaggct
     2581 gaaaaaagtt tcagaaggcc ataaaaggaa aacccccata atcttctcat cgaacttggc
     2641 ggaacgttag aagattatgg gggcctcaca gaatacggga taagtatata tgaaaccgtg
     2701 tcagagattc aacccttcgc agtgtattaa cgcacggcat tctgtttccg ccataagcga
     2761 cagcttgtgg caggtctgaa gaatactcca tataacgcag tacactggag tcagttagca
     2821 cccgaagagc agatccgttt ctgggaagac tatgaagcgg gaagggcgac ctctttcctg
     2881 gttgaaccgg aaaggaagcg caccaggcgc cgtcgtggcg aacactccac taaacccaaa
     2941 tgcgaaaatc cgtcctggta tcgtcctgcg cgctataagg agctgagcgg gcagctcggg
     3001 cacgcctaca accgtctggt gaaaaaggcc ccggtgaccg gcgagcagag cctgcgtatg
     3061 cgtatgtcgc ggcatccttt ttacgtgcag aaacggacgt tcgtcggtcg caaatatgct
     3121 ttccggccgg aaaaacaacg cctcctcgat gccatctggc cggttctggt cagcttcagt
     3181 gatgccggca cacacactgt ggggatgagt gtcactcgcc tggctaaaga aatcagtccg
     3241 aaagacagta agggaaatgt tattccggaa ctggaagtga cggtctcccg tctttcccgt
     3301 ctgctggccg aacaggtgcg ttttggtgtg ctgggtgttt cagaggaaac gatgtgggac
     3361 cgtgaaaccc gccagcgtct gccgcgctat gtctggataa caccggctgg ctggcagatg
     3421 ctgggcgtcg acatggtaaa acttcacgaa cagcagcaga aacgactgcg tgaaagtgaa
     3481 atccgccagc agctcattcg ggaaggtgtt ctgcgtgagg atgaagatat ctccgtacat
     3541 gcggccagaa aacgctggta tctgcagcgc agtcaggatg cgctgaaaca ccgtcgtgcg
     3601 aaagcagcag ccagtaagcg cgccagacgc ctgaagaaac tgcctgccga ccagcagatt
     3661 catgagatgg cagagtatct caggaagcgt ctgccgccgg atgaagccta tttttgttcc
     3721 gatgaccatc tgaagcgact ggccatcagg gagttgcgtc agcttgaact gacgctggct
     3781 gccccgccac cgcactagac agcaccattc cctcagcact gaatcatcgc cagcccctcc
     3841 ggggctttcg gcgcaggtat cgctcagccc aaaatccgca gtaatcacct taaatcccct
     3901 cagaggggca tatttgctca taaaaccacg catcagtcat cagaacgtgg ccacgttgtt
     3961 tcagttatcc acataaatcc gcaaacaaag aactttaaga agctgcaaac ctgaaacagc
     4021 aaacctgcaa tatagtctta accccattat ttaatcccct gcgttgcttc gccgcagcga
     4081 aagtctttat ctctgaaacc actgtgaaca aacacaaaga ggccttcgct tgcagcggcc
     4141 aaggccgcgc cgctcagaat ctaaaagcac ctcccacgct gacgcgcggg ccccgaacct
     4201 caccgttctg gaaccacaac aaaaaaacat caggaataaa aacaccacac aaacgcagca
     4261 ccgtgcccac ccctcataac tgaaaagcgc cgccgccccg gcccaaaggg ccggaacaga
     4321 gtcgctttta attatgaatg ttgtaactaa acatcatcat cgctttaaga ccccatttgt
     4381 ctagcagggc gatggcccac tgcgtgaacc atcaccctgc acacagggta ttttatgtgc
     4441 aattgtcaac cactgaatcg gaaccccaga ggggccccca cgattcagaa gttgacaatt
     4501 taagcacagg gactacatga gaaaaatcgt ggttcgtctg tcatacacgc gcgtaagcgg
     4561 cactgatagc cctcttacgc ggaaatacgc ccataagcgc taacttaagg gttgaaccga
     4621 cgccacggtg tctcgttaag acttcaattg cgttttgtag gcagtcaggg atactgtccc
     4681 atgaatggcc acctgtaagc tccagataac catttttgtt attctccaca acgaattccg
     4741 cataccggaa atcagcgcta cttctgggga ggaatggcct taagagtgat aaagattata
     4801 tgcttttcat agagagactc atctatgccg gtaacactga aaaatgtatt gtgttccaag
     4861 aagttttttt gagtgaacat attttcgctc acaacaatcg aattattcaa agccacaatt
     4921 ttaaaaaata ttttatatat atcactattt tgttcacgac agacatcttt actaaaagaa
     4981 ttaaagctaa attcaatatg ttcataatta gataaattta ttgtatctcc aacagctaca
     5041 ctttcttttg gccaaaaacg acatgttgag tatgcatata taggtaatga catctgagct
     5101 acaccgtctt ttccattatg ctcagaaaca gtatgtatat atctattgat aatactattc
     5161 tgtaaatatg aatacgttgg atttctatat gcctcaacca aatcaaataa ttcatgtgaa
     5221 tatgaagaag gaa
//
LOCUS       DAZFWL020000225         5159 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFWL020000225 DAZFWL020000000
VERSION     DAZFWL020000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.226"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1129
                     /locus_tag="ACHKNP_RS23865"
                     /old_locus_tag="pgaptmp_004773"
     CDS             <1..1129
                     /locus_tag="ACHKNP_RS23865"
                     /old_locus_tag="pgaptmp_004773"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGU4706781.1"
                     /translation="NKLDSIQVLRGIAVLLVIAFHFRVYLNGVYAQKDLGDILFGIGE
                     VGVDIFFVISGFIITYSSMNKAKNRPMVFAAKRFFRLYPVYFIILTLAIYLNYEEIYT
                     SSQIIKSYLLIPIDYNFIGPWSGYSIIIPAWTLTYEIWFYVVFFFSLCISHKYRTAIA
                     SFLLIIICVLGQLYFHGDVFLNPIAQPPSGDGIIRNLVFVSNPIIYDFILGMIIAEAY
                     IRLPSRLINKMPVVQFLNITLAIALLFLCLGLRNGPGIVAWGAYSFMIIAPIVLISKC
                     RDVYRNKILTYVGEISYSLYINHMVVYSLAVLYLKPYGVLDSKGFGVFLAMICATFII
                     SIISYNFIEKPSVSIGHAIARRMQKKSDVSSIKTESVREAP"
     gene            complement(1374..2531)
                     /gene="intS"
                     /locus_tag="ACHKNP_RS23870"
                     /old_locus_tag="pgaptmp_004774"
     CDS             complement(1374..2531)
                     /gene="intS"
                     /locus_tag="ACHKNP_RS23870"
                     /old_locus_tag="pgaptmp_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HGU4706782.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMRGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDANVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2693..2767)
                     /locus_tag="ACHKNP_RS23875"
                     /old_locus_tag="pgaptmp_004775"
     tRNA            complement(2693..2767)
                     /locus_tag="ACHKNP_RS23875"
                     /old_locus_tag="pgaptmp_004775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2732..2734),aa:Arg,seq:cct)
     gene            complement(2843..3775)
                     /gene="yfdC"
                     /locus_tag="ACHKNP_RS23880"
                     /old_locus_tag="pgaptmp_004776"
     CDS             complement(2843..3775)
                     /gene="yfdC"
                     /locus_tag="ACHKNP_RS23880"
                     /old_locus_tag="pgaptmp_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGU4706783.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4069..4824
                     /gene="mlaA"
                     /locus_tag="ACHKNP_RS23885"
                     /old_locus_tag="pgaptmp_004777"
     CDS             4069..4824
                     /gene="mlaA"
                     /locus_tag="ACHKNP_RS23885"
                     /old_locus_tag="pgaptmp_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HGU4706784.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(5006..>5159)
                     /locus_tag="ACHKNP_RS23890"
                     /old_locus_tag="pgaptmp_004778"
     CDS             complement(5006..>5159)
                     /locus_tag="ACHKNP_RS23890"
                     /old_locus_tag="pgaptmp_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706785.1"
                     /translation="NKVDVDHPENKHKDFKDLEDNVEKRLQNRQAKYQNEINQLSAPG
                     VNFDDI"
BASE COUNT         1313 a         1140 c         1152 g         1554 t
ORIGIN      
        1 aaataagtta gactctattc aagtccttag agggatcgcg gtacttctag tcattgcgtt
       61 tcactttaga gtatatttaa atggagtgta tgcccaaaag gacttgggcg acattctttt
      121 tggtataggt gaggttggtg ttgatatatt ctttgttata agtggattta tcattactta
      181 ttcttcaatg aataaggcaa aaaaccggcc aatggtgttt gctgctaagc gtttttttag
      241 gttgtaccct gtttacttta taatccttac tctggctata tacctcaact atgaggaaat
      301 atatacatca tcacaaataa taaagagcta cttgctcata ccaattgact ataattttat
      361 agggccatgg tcaggttata gtataataat acctgcatgg acactaacgt atgaaatttg
      421 gttctatgtg gttttctttt tttcattatg catcagccat aaatatagaa ctgcgatcgc
      481 atcatttctt ctcatcatta tttgcgttct tggtcaactg tactttcacg gagatgtttt
      541 tctaaatcct atcgcgcaac cgccatctgg tgatggaatt attcgtaatc tggtatttgt
      601 atcaaatcca ataatatatg actttatttt ggggatgatt attgcagagg cctacattag
      661 gctaccttct agacttataa ataaaatgcc tgttgttcaa tttcttaata ttacattagc
      721 gattgccttg ctttttttat gtctagggtt aaggaatgga cctggaattg tagcatgggg
      781 ggcttattca tttatgatta tagccccaat agtccttatt tctaagtgta gggatgtata
      841 tagaaataaa attctaacgt atgttggtga aatatcctat tcactttaca ttaatcatat
      901 ggttgtatac agtcttgctg ttttatatct caaaccgtac ggggtattgg atagtaaagg
      961 atttggtgtt tttctagcta tgatttgcgc tacttttatt atatcaatca tatcttataa
     1021 ctttattgaa aaaccatcag tatccatagg gcatgccatc gctcgtagaa tgcaaaaaaa
     1081 atcagatgta tcttcaatta aaactgaatc cgttagagaa gcaccttgat aaatagagca
     1141 atagccagat agcacctaac cgatttcgta ctcatagggt tgtcacttta ccaaactgag
     1201 aggaaataca ctgataacag ttggttatga agtactgggt gaggtggatg tgaatggtat
     1261 tttgatgcat ttcattaacc tgtacgccta agaaagcaac cctgaaatct aagacacaca
     1321 agctttgcac tggattgcaa ggctttgtgc acttcgatag tggttaaggt ggttcactcc
     1381 accttttcat caagccagtc cgcccaccat tgcatcattt ctctgcgctt atcgagatac
     1441 tgagcatggt tgtaaatacc gcgcacagat ccgccgttgg catgtgccag ttgcacttca
     1501 atagcgtcag caggccattc gtgctcgttc ataatcgtgc tgaattcatg cctgaatccg
     1561 tgaccgcttt ccagaccctc atagccgatt tgtttgatca caagtagcac cgcgttctcg
     1621 cagattggct tcttcttatc gttgcgcccg gcaaaaacaa attctgatac tggtttggtg
     1681 atggagctta acgtagtgag aagttcaacc acctggtccg acatcgggac aacatgaatt
     1741 ttgcgtccct tcatcacatt ggcgtcaatg gtgataatcc tgttttcaaa atcgacgttc
     1801 ttccattgca tggaacgaag ctcttttgtt cttagggctg tgtagcgtaa aaccttggtg
     1861 gcaatgagcg atacgatact tcctgaaaat gttgccagtg ctttattgaa tgccgggatc
     1921 tggtctgcag gaagaaacgg gaagttcttc ttgcggtatc ccctcatggc gtcagcaagg
     1981 tcaggtgccg ggttatattt agccctgccg gtgacaatag cgtaacggaa aacctcgccg
     2041 catcttctgc gggctttatt tgctcgctcc attgcaccgc gatcttcaaa tctgcggatt
     2101 acttccagca gttgcatcgg ctcaatatcc tgaatttcaa ggccgccgat gataggcaaa
     2161 atgtcgtcat caaacatttt tgcaagttca gtcgcatacc ctactgacca tacttgcttc
     2221 ttgtgctcgt accattcctt gtaaatcgca ctaaaggaat tgttgttaga cgaagccttt
     2281 ttcgccttta ccggatcgat gccaaccgag atgtctttcc tcgcagtcca tgccttatcc
     2341 cttgcctcct gcaaagtcat tagcggatat tttccgacgg tcaggatttt ctccttaccg
     2401 tcaatcttgt agcgaagctg ccataccttt ttcccggata caggaacata aaggtacagg
     2461 ccattaccat cgagaaggcg gtatggtttt tctttcggct ttgctgcttc aatctgctta
     2521 acggtgagca tgggtaaaaa tccggagggt aaaattattt tatccacttt ttacccgtca
     2581 tggggtgcgg ctgtcaacga tctgacgcga accatgacga actgtgaatc tacggaaggc
     2641 ttgatattca ggggattttg cggactggta cggatgggag cgaactgata aatggtgtcc
     2701 cctgcaggaa tcgaacctgc aattagccct taggaggggc tcgttatatc catttaacta
     2761 agaggacaat gcggcatgag tatacccgct aatggactgc ggggtaagta cgctgccgct
     2821 cgattgctta aaccctcgcc atttatgctg ggtttttatc atttttctta atgttttccg
     2881 cacgttctgc tttttggcgt gcttctgctt tacgcttgtt gctcatgtcg ttacgaatct
     2941 gtgcatgact cattaacgcg aagataaagg tgccgccgca gatgttcccc gctaaagtag
     3001 gtagtgcgaa gggccagatg aaatcgctcc agtgcagcgt gccgttaaac accagataga
     3061 ggatttcaac agaaccgaca acgatgtggg tggtgtcacc cagtgcaata agccaggtca
     3121 tcaaaataat caccacaatc tttgccgcac ccgctgcagg aaacatccaa accatagtgg
     3181 cgatcagcca gccggaaatg atcgcgttgg caaacatctc gctgggggtg ttcttcatca
     3241 catccatgcc gattttgaca aatgcatcgc gagtttcttc attgaagata ggcatatatt
     3301 caaatgccca tgcagcaata cctgtcccga gaatattacc cagcagcacg acgccccata
     3361 atcgcataag taagccgacg ttgctcattg tcggtttttg catgacgggt agtaccgcag
     3421 tcacggtgtt ttcggtaaat aattgctggc gggccataat gacgataata aaaccaaagg
     3481 tataaccgag attctccagt aagaagctgc ctggcactcc ttccagttcg acatgaaata
     3541 tcccttttgc cagtagcgaa gcgcccatcg acagacccgc cgcaatggct gaccacagta
     3601 gcgccattgc gtcgcgttcc agctcttttt caccatcctg gcggatatgc tcatgaattg
     3661 ccatcgcccg ggaggggagt cggtcttcat ctatttctat ttttttgccg cgctcttttt
     3721 cttcgctctc aacttcaatt tcgtcgctgt gttgatcaat tttgtcgttg tccatggtct
     3781 cttctcttga attagcacgt atagctaaag cgtagcggct tttttgctcg caactggcgg
     3841 gagttactct gaaaatgtag aaaaggctgc gtttgccttt ttctgtttct atagaatcaa
     3901 gtagcctaca gggcggcgat taccaggcta tgatcaaatc agcaaatcag ggcgtctgga
     3961 catcagttga cgtgctgtta caatcgccca cacctaaaca ggcggatacg gtatcgttcc
     4021 gtcatggatg gcaaactgca taagccataa aaaaacaggg agacatttat gaagcttcgc
     4081 ctgtcggcgc ttgctctggg aactacgctt ctggtggggt gtgcgagttc cggtacagat
     4141 cagcaagggc gttctgaccc gttagaaggg ttcaaccgca ccatgtacaa cttcaacttc
     4201 aacgtattag acccgtatat tgttcgaccg gtcgctgtcg cctggcgtga ttatgttccg
     4261 caaccggcgc gtaatggttt gagcaacttt actggcaacc ttgaagaacc tgcggtgatg
     4321 gttaactact tcttgcaggg cgacccttat caggggatgg tccactttac ccgctttttc
     4381 ctgaacacca ttttggggat gggcggcttt attgatgttg cagggatggc gaacccgaaa
     4441 ctgcaacgga ctgaacctca ccgcttcggt agtacgcttg gtcattatgg cgtgggttat
     4501 gggccttacg ttcagttacc gttctacggt agcttcacgc tgcgtgatga cggtggtgat
     4561 atggcggatg gtctttaccc ggttctttcc tggctgacct ggccgatgtc tgtgggtaaa
     4621 tggacgcttg aagggatcga aacccgcgct cagctgctgg attccgatgg tctgctgcgt
     4681 cagtcgtccg atccttatat tatggtgcgc gaagcgtact tccagcgtca tgatttcatc
     4741 gctaatggcg gcgaactcaa accgcaggaa aatccgaacg cacaagcgat tcaggatgat
     4801 ttaaaagata ttgattctga ataagaaaca aataaaaaag gtgagtcgca aaactcacct
     4861 tttttgttgt tatccctcaa ttaccacaag taaaaggaga taaaccgaca atgacccgga
     4921 cgtaatgatg taaaaatact gatgttcgtc actgactatt tccgatattc aggcgttgaa
     4981 aaatcaaaaa actggaaaaa taattttaaa tatcatcaaa attaacacct ggcgcagata
     5041 gttgattaat ttcattttga tatttcgcct ggcgattttg aagtcttttt tcaacgttat
     5101 cttcgagatc cttaaagtct ttgtgtttat tttcaggatg gtcaacgtca actttgtta
//
LOCUS       DAZFWL020000226         5133 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFWL020000226 DAZFWL020000000
VERSION     DAZFWL020000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.227"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(35..1054)
                     /gene="rspB"
                     /locus_tag="ACHKNP_RS23895"
                     /old_locus_tag="pgaptmp_004779"
     CDS             complement(35..1054)
                     /gene="rspB"
                     /locus_tag="ACHKNP_RS23895"
                     /old_locus_tag="pgaptmp_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HGU4706786.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(1066..2280)
                     /gene="rspA"
                     /locus_tag="ACHKNP_RS23900"
                     /old_locus_tag="pgaptmp_004780"
     CDS             complement(1066..2280)
                     /gene="rspA"
                     /locus_tag="ACHKNP_RS23900"
                     /old_locus_tag="pgaptmp_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HGU4706787.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(2486..2812)
                     /gene="ynfA"
                     /locus_tag="ACHKNP_RS23905"
                     /old_locus_tag="pgaptmp_004781"
     CDS             complement(2486..2812)
                     /gene="ynfA"
                     /locus_tag="ACHKNP_RS23905"
                     /old_locus_tag="pgaptmp_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HGU4706788.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            2947..3288
                     /gene="ynfB"
                     /locus_tag="ACHKNP_RS23910"
                     /old_locus_tag="pgaptmp_004782"
     CDS             2947..3288
                     /gene="ynfB"
                     /locus_tag="ACHKNP_RS23910"
                     /old_locus_tag="pgaptmp_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HGU4706789.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            3323..3883
                     /gene="speG"
                     /locus_tag="ACHKNP_RS23915"
                     /old_locus_tag="pgaptmp_004783"
     CDS             3323..3883
                     /gene="speG"
                     /locus_tag="ACHKNP_RS23915"
                     /old_locus_tag="pgaptmp_004783"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HGU4706790.1"
                     /translation="MPSAHSVKLRPLEREDLRYEHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(3886..4596)
                     /gene="ynfC"
                     /locus_tag="ACHKNP_RS23920"
                     /old_locus_tag="pgaptmp_004784"
     CDS             complement(3886..4596)
                     /gene="ynfC"
                     /locus_tag="ACHKNP_RS23920"
                     /old_locus_tag="pgaptmp_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HGU4706791.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4704..5009
                     /gene="ynfD"
                     /locus_tag="ACHKNP_RS23925"
                     /old_locus_tag="pgaptmp_004785"
     CDS             4704..5009
                     /gene="ynfD"
                     /locus_tag="ACHKNP_RS23925"
                     /old_locus_tag="pgaptmp_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HGU4706792.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         1406 a         1301 c         1118 g         1308 t
ORIGIN      
        1 taagatgcgt actacttact cgccgttatt ggtattattc agaaaaagtg agcaagactt
       61 tgcagcaatg tttttgatcc tgttcaaata aactaatggc atcagcaaca tgctggaaat
      121 caaacgtatg ggtaattaat ttttctggtt taattaaccc tttacttaac cagtcgataa
      181 caaccggaaa tttatttgca tttaagcgtg aagagaaaat agagagttct tttccggtaa
      241 ttccttgctg aatcacttca gacggttcac tggagaagcc catcaataca atacgtgccg
      301 ctggagaagc cagcgttacg gcctctttca ggatagaagg atgacaagcc gcatcgataa
      361 ttaatgtcgg cttgatgcct ttttcagcga aaatctcgcc aagcggtgtc tggctgttat
      421 taatcgccca gtctgccccg ctctctttcg ctttttccag tcgttcatca atgcgatcgg
      481 caacaatcac atttttaacg ttatagacgc cttttaatac ctgaacgatc gtcaggccga
      541 ttggaccggc accataaacc agaacggtat cattttcagt cggttgacca tgaccggtaa
      601 cgttagccgc aatggtaaaa ggttcgatca tcaccgcata ttgatcggcc actgcttcag
      661 gaattttcca cgcatttttt gccggaacca cggcatattc actgaaacca ccgtcagcgt
      721 gcacacctaa tacagcaagt gtcgtacaaa cgttcggctt acctatagag cacggatagc
      781 aatgcccaca gctgaccacc ggatcgacag caacgcgctc accgactctg gcgctttcca
      841 caccttcacc caccgcatca atgacgccaa agaattcatg accaatgacg cgcggatatt
      901 tcgcaaaagg attatgccca cggtaaatat ggctatctga accacaaatt ccggcaagtt
      961 tcacttttac tcgtacttca cccgctgacg gggtgggtat ttcacgttcg ataatcgaca
     1021 gttgattcgg tttttcaatt aaaatgcttt tcattacctt actccttacc agttccacag
     1081 cgtgccatct tccagacgtg cgactggcag ataagcaggt tcatagggat atttcgccgc
     1141 cagcttttca tcgaattcga tgccaagacc cggtttgtct cccggatgca tatagccgtt
     1201 atcgaaagtc cagttgtgcg ggaagacttc gagcatttgt tcggaataac ccatgtactc
     1261 ctggacaccg aaattgggga cccacagatc aaagtgcagc gccgcagcca tgcagactgg
     1321 tgacaaatcg gaaggaccgt gtgagccagt acgtacctga tacagcgaag caaaatcggc
     1381 aatccggcgc ataccggtaa ttccgcctgc atgggtcagc gtggtgcgga tataatcgat
     1441 gagttgctct tcaatcagtt gtttgcagtc ccagatgctg ttgaagactt cacccactgc
     1501 gatgggtgtg acggtatgtt ggcgaatgag acggaagcat tcctggtttt ccgcaggcgt
     1561 cgggtcttcc atccagaaca tgcgataatc ttcaatgctt ttaccaaagc gcgccgcttc
     1621 aataggcgtt aagcgatggt gcatgtcatg cagcaaatgt tcattaaaac caaacttgtt
     1681 acgtaccgcg tcaaacaatt tcggcatgaa atcgaggtat ttctccgtcg accacagctg
     1741 ctcttccggc cactgtcctt tggttgcggg ttcataagcc agacctttac ctttcgacat
     1801 gccgtaggtg gttttcatac cagggattcc gcactgcacg cggatggctt tgaatcccag
     1861 ctcctgatga cgggcataat catccagagc ttcatcaata ctgtgaccgg tggtatggca
     1921 ataaaccatc accccttcac gagacgcgcc gccgagtaac tggtaaagcg gcatgttggc
     1981 agctttggct ttaatatccc acagcgccat atcaaccgct gaaatggccg acatcgtaac
     2041 cggaccgcga cgccagtaag cacctttata gaaaaactgc cagatatctt cgatacggtg
     2101 cgcatcgcga ccaataagct gcggacaaag gtgatcctgc aaataagagg ccacggaaag
     2161 ctcacgtcca ttgagggtgg catccccaag gcccgtaata ccgtcctcag tggtgatttt
     2221 taatgtgacg aaattacgcc ccggacaggt aacaaaaact tcagccttta cgatcttcat
     2281 gttcgattcc ttgcatcgct tgtcgtgatg catgaaatct acgcaactga gctactacca
     2341 tacaagcata aagatcgaaa aaagccggag tgatcacaaa aaaaggcgta tatttgcgct
     2401 gtgaatggtt gacaaaagat gaaatagaat accttttgtc agctgacact tcctcttatc
     2461 ttattgataa aatggattta tgttcctacg tgcgccccca gcccgcaaca atgatcaaca
     2521 tgccgcaaag cgcaatcaac gcacccgtcc agtcataaag agtcagtttc acgccatcca
     2581 caacgcgcag ccacatcaac gccgtgcaga cataaacgcc accataagcc gcgtaaacac
     2641 gcccactcgc cgctggatgc aacgttaaca accagacaaa cagcgccagt gaaatccccg
     2701 ccggaagcaa cagccagata ctggcgtttc gttttaacca caaccagggc agaaagcatc
     2761 caataatttc acacagcgca gtagcaaaaa atagtaacgt tgttttaatc atctttgtct
     2821 cttattgaca tcatgtatag ttatagggcg acataatatc atcaatataa acaccctcct
     2881 ggtacgtttt gcgtccgcag tggatggtgt agaatcacct ttaatcattc atacagggaa
     2941 tgaattatga aaatcactct cagcaaacga atcggcctgc tcgctattct gctgccttgc
     3001 gcactggcat tgagcacaac tgttcatgcc gaaactaaca aactggtgat tgagtctggc
     3061 gacagtgcac aaagccgcca gcacgccgct atggaaaaag agcaatggaa tgacacgcgc
     3121 aatctgcgcc agaaagtgaa taaacgcact gaaaaagagt gggataaagc cgacgccgct
     3181 tttgataacc gcgataaatg tgagcaaagc gccaacatca atgcctactg ggagcccaat
     3241 actttgcgct gcctggaccg tcgaactggc cgcgttatta ccccctaacc tgttattgat
     3301 ttaaggaatg taaggacacg ttatgccaag cgcccacagt gttaagctac gcccgctgga
     3361 gcgtgaagat ttacgctatg aacatcaact cgacaacaac gccagtgtga tgcgttactg
     3421 gtttgaggaa ccctacgaag cctttgttga actctctgat ctgtatgata agcatattca
     3481 cgatcagagc gaacggcgct ttgtggtgga atgtgacggc gaaaaagccg gtctggtgga
     3541 gctggtggaa attaaccatg ttcatcgccg cgcagaattt cagataatta tctccccgga
     3601 gtatcagggg aaaggtctgg caacccgtgc cgccaaatta gcaatggact atggctttac
     3661 cgttctcaat ctctataagc tgtatctgat cgttgataaa gagaatgaaa aagcgattca
     3721 catttaccgc aagcttggct tttcggttga aggtgaattg atgcacgagt tctttattaa
     3781 tggtcaatat cgtaatgcca ttcgcatgtg tatattccag catcagtatc tggcagagca
     3841 caaaacaccg ggtcagactc tcctgaagcc gaccgcacaa tagcattaat aataatcgat
     3901 cgtatttttg atggtgtaaa cccgttcgac ggcgggtttt actccttcat caacaatgat
     3961 tagctgacag tccaccggat tagcgtgact gtcatattca cagctctgtt ttacattacc
     4021 aacccgttga ttattcagta aagtaaccgc tgtgtaatct aattttttga tcggatccgt
     4081 tgatggcgtg gcgctgacag ataatgtttt gtcgttactt ttcgtggttt tacccagcgg
     4141 ataaccctga tcatcatagc gatattccat ctgcatttgt ttgctgctgg ctttaatcac
     4201 gaagccatta tcatcggttt cccaactcac cccggcagaa ggtaattctg ctagctggca
     4261 ttttccctgt aaacgtactc tcttctccag cgtctcggca tcacggtaat aattggcgtc
     4321 cagtaccaga gcgaccacgg tattattttc cagatccagt aattcgagtg aatcaaaaca
     4381 gccttcttcc gacaaagtcc cagaaacacg tttcgtcact tcaccttgct catccattaa
     4441 tgtctgagtg aaatctttta ccggaccacg cagcggatca aaatcgaatt cattagagaa
     4501 actggccatc tcaggggtaa atgaaagtgt tacctctgtg cggtcacatc ctgtgaggaa
     4561 tatcgcgagt aagcatggta gtaatttgta tttcacaaca gtcaccagag agtagagatg
     4621 attctcaatc atagtagcaa atacagtact ttacacgtta aatgctatgc ttaaagaagt
     4681 tatcttcgcg taaggagctt atgatgaaac tctcaacctg ctgtgccgcg cttctgctcg
     4741 ccctcgcctc acccgtggta ctcgccgcgc cgggatcctg tgagcgcata cagagcgata
     4801 tatcacagcg cattatcaat aatggcgtac ccgaaagcag cttcacgtta agtattgtac
     4861 ccaatgacca ggttgatcag cctgattccc aggtcgtcgg ccattgcgct aatgatacgc
     4921 ataaaattct ctatacccgc accaccagcg gtaacgtctc tgctcccgcg cagtctaccc
     4981 aggatggcgc gcctgccgaa ccgcagtaat acattatcga ctgaacgccg gatacgacac
     5041 atccggcgat ttgaacatac aacataatcc caccttatta ctcataccct tctattgata
     5101 tggattaata attcttaacc caaaatgggt aga
//
LOCUS       DAZFWL020000227         5123 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFWL020000227 DAZFWL020000000
VERSION     DAZFWL020000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.228"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..834
                     /locus_tag="ACHKNP_RS23930"
                     /old_locus_tag="pgaptmp_004786"
     CDS             <1..834
                     /locus_tag="ACHKNP_RS23930"
                     /old_locus_tag="pgaptmp_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4706793.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVANNITQSG
                     GTEWSKRDKSTYDLTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKLDTRRGS
                     HSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDAFVSVQL
                     A"
     gene            876..1271
                     /locus_tag="ACHKNP_RS23935"
                     /old_locus_tag="pgaptmp_004787"
     CDS             876..1271
                     /locus_tag="ACHKNP_RS23935"
                     /old_locus_tag="pgaptmp_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4706794.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            1283..1657
                     /locus_tag="ACHKNP_RS23940"
                     /old_locus_tag="pgaptmp_004788"
     CDS             1283..1657
                     /locus_tag="ACHKNP_RS23940"
                     /old_locus_tag="pgaptmp_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4706795.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRRWKGDVWP"
     gene            1648..2226
                     /locus_tag="ACHKNP_RS23945"
                     /old_locus_tag="pgaptmp_004789"
     CDS             1648..2226
                     /locus_tag="ACHKNP_RS23945"
                     /old_locus_tag="pgaptmp_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4706796.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVSQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            2223..2618
                     /locus_tag="ACHKNP_RS23950"
                     /old_locus_tag="pgaptmp_004790"
     CDS             2223..2618
                     /locus_tag="ACHKNP_RS23950"
                     /old_locus_tag="pgaptmp_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4706797.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSELITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            2626..3366
                     /locus_tag="ACHKNP_RS23955"
                     /old_locus_tag="pgaptmp_004791"
     CDS             2626..3366
                     /locus_tag="ACHKNP_RS23955"
                     /old_locus_tag="pgaptmp_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4706798.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATDMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            3382..3804
                     /locus_tag="ACHKNP_RS23960"
                     /old_locus_tag="pgaptmp_004792"
     CDS             3382..3804
                     /locus_tag="ACHKNP_RS23960"
                     /old_locus_tag="pgaptmp_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4706799.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            3786..4220
                     /locus_tag="ACHKNP_RS23965"
                     /old_locus_tag="pgaptmp_004793"
     CDS             3786..4220
                     /locus_tag="ACHKNP_RS23965"
                     /old_locus_tag="pgaptmp_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4706800.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            4213..>5123
                     /locus_tag="ACHKNP_RS23970"
                     /old_locus_tag="pgaptmp_004794"
     CDS             4213..>5123
                     /locus_tag="ACHKNP_RS23970"
                     /old_locus_tag="pgaptmp_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU4706801.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARA"
BASE COUNT         1134 a         1312 c         1641 g         1036 t
ORIGIN      
        1 tcccgtggcg gctccacctc tgaatttacg ccgggatatg tcaaacccaa gcatgaagtg
       61 aatccgcaga tgaccctgcg tcgcctgccg gatgaagatc cacagaatct ggcggacccg
      121 gcttaccgcc gccgtcgcat catcctgcag aacatgcgag acgaagagct ggccattgct
      181 caggtcgaag agatgcaggc agtttctgcc gtgcttaagg gcaaatacac catgaccggt
      241 gaagccttcg atccggttga agtggatatg ggccgcagtg tggcgaacaa catcacgcag
      301 tccggcggta cggagtggag caagcgtgac aagtccacgt atgacctgac cgacgatatc
      361 gaagcctacg cgctgaacgc cagcggtgtg gtgaatatca tcgtgtttga tccgaaaggc
      421 tgggcgctgt tccgttcctt caaagccgtc aaggagaagc tggatacccg tcgcggctct
      481 cattccgagc tggagacagc ggtaaaagac ctgggcgaag cggtgtccta taaggggatg
      541 tatggcgata cggcgatcgt cgtgtattcc ggacagtacg tggaaaacga cgtcaaaaag
      601 aacttccttc cggacaacac gatggtgctg gggaacactc aggcacgcgg tctgcgcacc
      661 tatggctgca ttcaggatgc ggacgcacag cgcgaaggta ttaacgcctc tgcccgctac
      721 ccgaaaaact gggtgaccac cggcgatccg gcgcgtgagt tcaccatgat tcagtcagca
      781 ccgctgatgc tgctggctga ccctgatgcg ttcgtgtccg tacaactggc gtaatcatgg
      841 cccttcgggg ccattttctc tctgtggagg agtccatgac gaaagatgaa ctgattgccc
      901 gtcttcaggt gctgggtgag caactgaacc gtgatgtcag cctgacgggg acgaaagaag
      961 aactggcact ccgtgtggca gagctggaag aggagcttga tgacacggat gacgctgccg
     1021 gtcaggacac atctgtcagc ccggaaaatg cgctgaccgg acatgaaaat gaggttgtat
     1081 cagcgcagcc ggataccgtg actgatacgg ctgatctggt cacggttgtg gcactggtga
     1141 cgctgcatac tgatgcactt cacgccacgc gggatgaggc tgtggcattt gtgctgccgg
     1201 gaacggcgtt ccgtgtctct gccggtgtgg cagctgaaat gacagagcgc ggcctggcca
     1261 gaatgcaata acgggaggcg ctgtggctga tttcgataac ctgttcgatg ctgccattgc
     1321 ctgcgccgat gaaacgatac gcgggtacat gggaacgtca gccaccatga catccggtga
     1381 gcagtccggt gctgtgatac gtggtgtttt tgatgaccct gaaaatatca gctatgccgg
     1441 acagggcgtg cgcgttgaag gctccagccc gtccctgttt gtccggactg atgatgtgcg
     1501 gcagctgcgg cgcggcgaca cgctgaccat cggtgaggaa aacttctgga tagaccggat
     1561 ttcgccggat gatggcggaa gctgtcatct ctggcttggg cggggcgtac cgcctgccgt
     1621 taaccgtcgc cgctggaagg gggatgtatg gccataaaag gtcttgagca ggccgttgaa
     1681 aacctcagcc gtatcagcag aacggcggtg cccggtgccg ccgcaatggc cattaaccgc
     1741 gttgcttcat ccgcgatatc gcagtcggtg tcacaggttg cccgtgagac aaaggtacgc
     1801 cggaaactgg taaaggaaag ggccaggctg aaaagggcca cggtcaaaaa tccgcaggcc
     1861 agaatcaagg ttaaccgggg ggatttgccc gtaatcaagc tgggtaatgc gcgggttgtc
     1921 ctttcgcgcc gcaggcgtcg taaaaagggg cagcgttcat ccctgaaagg tggcggcagc
     1981 gtgcttgtgg tgggtaaccg tcgtattccc ggcgcgttta ttcagcaact gaaaaatggc
     2041 cggtggcatg ttatgcagcg tgtggccggg aaaaaccgtt accccattga tgtggtgaaa
     2101 atcccgatgg cggtgccgct gaccacggcg tttaaacaaa atattgagcg gatacggcgt
     2161 gaacgtcttc cgaaagagct gggctatgcg ctgcagcatc aactgaggat ggtaataaag
     2221 cgatgaaaca tactgaactc cgtgcagccg tactggatgc actggagaag catgacaccg
     2281 gggcgacgtt ttttgatggt cgccccgctg tttttgatga ggcggatttt ccggcagttg
     2341 ccgtttatct caccggcgct gaatacacgg gcgaagagct ggacagcgat acctggcagg
     2401 cggagctgca tatcgaagtt ttcctgcctg ctcaggtgcc ggattcagag ctggatgcgt
     2461 ggatggagtc ccggatttat ccggtgatga gcgatatccc ggcactgtca gagttgatca
     2521 ccagtatggt ggccagtggc tatgactacc gacgcgacga tgatgcgggc ctgtggagtt
     2581 cagccgatct gacttatgtc attacctatg aaatgtgagg acgctatgcc tgtaccaaat
     2641 cctgtaatgc cggtgaaagg tgccgggacc accctgtggg tttataaggg gagcggtgac
     2701 ccttatgcga atccgctttc agacgttgac tggtcgcgtc tggcaaaagt taaagacctg
     2761 acgcccggcg aactgaccgc tgagtcctat gacgacagct atctcgatga tgaagatgcg
     2821 gactggactg cgaccgggca ggggcagaaa tcagccggag ataccagctt cacgctggcg
     2881 tggatgcccg gagagcaggg gcagcaggcg ctgctggcgt ggtttaatga aggtgatacc
     2941 cgtgcctata aaatccgctt cccgaacggc acggtcgatg tgttccgtgg ctgggtcagc
     3001 agtatcggta aagcggtgac ggcgaaggaa gtgatcaccc gcacggtgaa ggtcaccaat
     3061 gtgggacgcc cgtcgatggc agaagatcgc agcacggtca cagcggcaac cgacatgacc
     3121 gtgacgcctg ccagcacctc ggtggtgaaa gggcagagca ccacgctgac cgtggcattc
     3181 cagccggaag gcgcaaccga caagagcttc cgtgcggtgt ctgcggataa aacaaaagcc
     3241 accgtgtcgg tcagtggtat gaccatcacc gtgaaaggtg ttgctgcagg caaggtcaac
     3301 attccggtcg tatccggtaa tggtgaactt gctgcggttg cagaaatcac tgtcaccgac
     3361 agttaatccg gagagtcagc gatgttcctg aaaaccgaat catttgaaca taacggcgtg
     3421 accgtcacgc tttctgaact ttcagccctg cagcgtattg agcatctcgc cctgatgaaa
     3481 cggcaggcag aacaggcgga gtcagacagc aaccggaagt ttacagtgga agacgccatc
     3541 agaaccggtg cttttgtggt ggcgatgtcc ctgtggcata accatccgca gaagacaaag
     3601 cagccttcca tgaatgaagc cgttaaacag attgagcagg aagtgcttac cacctggccc
     3661 acagaggcaa tttctcatgc tgaaaacgtg gtgtaccggc tgtccggtat gtatgagttt
     3721 gtggtgaatg atgcccctga acaggcagag gacgccgggc ctgcagagcc tgtttctgcg
     3781 ggaaagtgtt cgacggtgag ctgagttttg ccctgaaact ggcgcgcgag atggggcgac
     3841 ccgactggcg tgccatgctt gccgggatgt catccacgga gtatgccgac tggcaccgct
     3901 tttacagtac ccattatttt catgatgttc tgctggatat gcacttttcc gggctgacgt
     3961 acaccgtgct cagcctgttt ttcagcgatc cggatatgca tccgctggat ttcagtctgc
     4021 tgaaccggcg tgaggctgac gaagagcctg aagatgatgt gctgatgcag aaagcggcag
     4081 ggcttgccgg aggcgtccgc tttggcccgg acgggaatga agttatcccc gcttccccgg
     4141 atgtggcgga catgacggag gatgacgtaa tgctgatgac agtatcagaa gggatcgcag
     4201 gaggagtccg gtatggctga accggtaggc gatctggtcg ttgatttaag tctggatgcg
     4261 gccagatttg acgagcagat ggccagagtc aggcgtcatt tttccggtac ggaaagtgat
     4321 gcgaaaaaaa cagcggcagt cgttgaacag tcgctgagcc gacaggcgct ggctgcacag
     4381 aaagcgggga tttccgtcgg gcagtataaa gccgccatgc gtatgctgcc tgcacagttc
     4441 accgacgtgg ccacgcagct tgcaggcggg caaagtccgt ggctgatcct gctgcaacag
     4501 ggggggcagg tgaaggactc cttcggcggg atgatcccca tgttcagggg gcttgccggt
     4561 gcgatcaccc tgccgatggt gggggccacc tcgctggcgg tggcgaccgg tgcgctggcg
     4621 tatgcctggt atcagggcaa ctcaaccctg tccgatttca acaaaacgct ggtcctttcc
     4681 ggcaatcagg cgggactgac ggcagatcgt atgctggtcc tgtccagagc cgggcaggcg
     4741 gcagggctga cgtttaacca gaccagcgag tcactcagcg cactggttaa ggcgggggta
     4801 agcggtgagg ctcagattgc gtccatcagc cagagtgtgg cgcgtttctc ctctgcatcc
     4861 ggcgtggagg tggacaaggt cgctgaagcc ttcgggaagc tgaccacaga cccgacgtcg
     4921 gggctgacgg cgatggcacg ccagttccat aacgtgacgg cggagcagat tgcgtatgtt
     4981 gctcagttgc agcgttccgg cgatgaagcc ggggcattgc aggcggcgaa cgaggccgca
     5041 acgaaagggt ttgatgacca gacccgccgc ctgaaagaga acatgggcac gctggagacc
     5101 tgggcagaca ggactgcgcg ggc
//
LOCUS       DAZFWL020000228         5102 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFWL020000228 DAZFWL020000000
VERSION     DAZFWL020000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.229"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..75
                     /locus_tag="ACHKNP_RS23975"
                     /old_locus_tag="pgaptmp_004795"
     CDS             <1..75
                     /locus_tag="ACHKNP_RS23975"
                     /old_locus_tag="pgaptmp_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4706802.1"
                     /translation="RMAEHIRYMVETIAHHQVDIDSEV"
     gene            81..866
                     /gene="bet"
                     /locus_tag="ACHKNP_RS23980"
                     /old_locus_tag="pgaptmp_004796"
     CDS             81..866
                     /gene="bet"
                     /locus_tag="ACHKNP_RS23980"
                     /old_locus_tag="pgaptmp_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGU4706803.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            863..1543
                     /locus_tag="ACHKNP_RS23985"
                     /old_locus_tag="pgaptmp_004797"
     CDS             863..1543
                     /locus_tag="ACHKNP_RS23985"
                     /old_locus_tag="pgaptmp_004797"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU4706804.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            1540..1722
                     /locus_tag="ACHKNP_RS23990"
                     /old_locus_tag="pgaptmp_004798"
     CDS             1540..1722
                     /locus_tag="ACHKNP_RS23990"
                     /old_locus_tag="pgaptmp_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGU4706805.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            1695..1886
                     /locus_tag="ACHKNP_RS23995"
                     /old_locus_tag="pgaptmp_004799"
     CDS             1695..1886
                     /locus_tag="ACHKNP_RS23995"
                     /old_locus_tag="pgaptmp_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU4706806.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            1963..2178
                     /locus_tag="ACHKNP_RS24000"
                     /old_locus_tag="pgaptmp_004800"
     CDS             1963..2178
                     /locus_tag="ACHKNP_RS24000"
                     /old_locus_tag="pgaptmp_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU4706807.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2277..2495
                     /locus_tag="ACHKNP_RS24005"
                     /old_locus_tag="pgaptmp_004801"
     CDS             2277..2495
                     /locus_tag="ACHKNP_RS24005"
                     /old_locus_tag="pgaptmp_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU4706808.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRQNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            2543..2821
                     /locus_tag="ACHKNP_RS24010"
                     /old_locus_tag="pgaptmp_004802"
     CDS             2543..2821
                     /locus_tag="ACHKNP_RS24010"
                     /old_locus_tag="pgaptmp_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4706809.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            2793..2858
                     /locus_tag="ACHKNP_RS24015"
                     /old_locus_tag="pgaptmp_004803"
                     /pseudo
     CDS             2793..2858
                     /locus_tag="ACHKNP_RS24015"
                     /old_locus_tag="pgaptmp_004803"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3020..4183
                     /gene="intD"
                     /locus_tag="ACHKNP_RS24020"
                     /old_locus_tag="pgaptmp_004804"
     CDS             3020..4183
                     /gene="intD"
                     /locus_tag="ACHKNP_RS24020"
                     /old_locus_tag="pgaptmp_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HGU4706810.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(4199..4275)
                     /locus_tag="ACHKNP_RS24025"
                     /old_locus_tag="pgaptmp_004805"
     tRNA            complement(4199..4275)
                     /locus_tag="ACHKNP_RS24025"
                     /old_locus_tag="pgaptmp_004805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4239..4241),aa:Arg,seq:tct)
     gene            4518..>5102
                     /gene="fimZ"
                     /locus_tag="ACHKNP_RS24030"
                     /old_locus_tag="pgaptmp_004806"
     CDS             4518..>5102
                     /gene="fimZ"
                     /locus_tag="ACHKNP_RS24030"
                     /old_locus_tag="pgaptmp_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HGU4706811.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLH"
BASE COUNT         1511 a         1168 c         1251 g         1172 t
ORIGIN      
        1 cgcatggcag aacacatccg gtacatggtt gaaaccattg ctcaccacca ggttgatatt
       61 gattcagagg tataaaacgg atgagtacag cactcgcaac gctggctggg aagctggctg
      121 aacgtgtcgg catggattct gtcgacccac aggaactgat caccactctt cgccagacgg
      181 catttaaagg tgatgccagc gatgcgcagt tcatcgcatt gctgatcgtc gccaaccagt
      241 acggccttaa tccgtggacg aaagaaattt acgccttccc tgataagcag aacggcatcg
      301 ttccggtggt gggcgttgat ggctggtccc gcatcatcaa cgaaaaccag cagtttgatg
      361 gcatggactt tgagcaggac aatgaatcct gcacatgccg gatttaccgc aaggaccgta
      421 atcatccgat ctgcgttacc gagtggatgg atgaatgccg ccgcgaacca ttcaaaaccc
      481 gcgaaggcag agaaatcacg gggccgtggc agtcgcatcc caaacggatg ttacgtcata
      541 aagccatgat tcagtgtgcc cgtctcgcct tcggatttgc tggtatctat gacaaggatg
      601 aagccgagcg cattgtcgaa aataccgcat acactgcaga acgtcagccg gaacgcgaca
      661 tcactccggt taacgatgaa accatgcagg aaattaacac tctgctgatc gccctggata
      721 aaacatggga tgacgactta ttgccgctct gttcccagat atttcgccgc gacattcgtg
      781 catcgtcaga actgacacag gccgaagcag taaaagctct tggattcctg aaacaaaaag
      841 ccactgaaca gaaggtggca gcatgacacc ggacattatc ctgcagcgta ctgggatcga
      901 cgtgagagct gtcgaacagg gagatgatgc gtggcacaaa ttacggctcg gcgtcatcac
      961 agcttcagaa attcacaacg taatagcaaa accccgatca ggaaagaagt ggcctgacat
     1021 gaaaatgtcc tacttccaca ccctgctggc tgaggtttgc accggtgtgg ctccggaagt
     1081 taacgctaag gctctggcct ggggaaaaca gtacgagaac gacgccagaa ccctctttga
     1141 gttcacttcc ggcgtgaatg ttactgaatc cccgatcatc tatcgcgacg aaagtatgcg
     1201 caccgcctgc tctcccgatg gtttatgcag tgacggcaac ggccttgagc tgaaatgccc
     1261 gtttacctcc cgggatttca tgaagttccg gctcggtggt ttcgaggcca taaaatcggc
     1321 ttacatggcc caggtgcagt acagcatgtg ggtgacacga aaagatgcct ggtactttgc
     1381 caactatgac ccgcgcatga agcgtgaagg cctgcattat gtcgtgattg agcggaatga
     1441 aaagtacatg gcgagttttg acgagatggt gccggagttc atcgaaaaaa tggacgaggc
     1501 actggctgaa attggttttg tatttgggga gcaatggcga tgacgcatcc tcacgataat
     1561 atccgggtag gcgcgatcac tttcgtctac tccgttacaa agcgaggctg ggtatttccc
     1621 ggcctttctg ttatcagaaa tccactgaaa gcacagcggc tggctgagaa gataaataat
     1681 aaacgggagg cggtatgcac aaagcatctc ctgttgagtt aagaacgagt attgagatgg
     1741 cacatagcct tgctcaaatt ggagtcaggt ttgtgccaat accagtagaa acagacgaag
     1801 aatttcatac gttagccaca tccctttcac aaaagctgga aatgatggtg gcgaaagcag
     1861 aagcagatga gagagaccag gtatgacaac cactgaatgc atttttctgg cagcgggctt
     1921 catattctgt gtgcttatgc ttgccgacat ggggcttgtt caatgacacc tcagcaagaa
     1981 aacgcccttc gcagcattgc ccgtcaggct aattctgaaa tcaaaaaagc cagacagcat
     2041 tttccggata aaaacgtcga tgacatttgc cgtagcgtac taaagaagca ccgcgaaacg
     2101 gtaacgctga tgggattcac accgactcat ttaagcctgg cgatcggcat gttaaacggc
     2161 gtctttaagg aacggtgaac atgaaaagca aaatcatcag ggagctacag gctccttttt
     2221 tattgttcgc attcatcctc aagcgtatta accaacaatt cagggattaa tggaagatgg
     2281 cagacatcat tgattcagca tcagaaatcg aagaattaca gcgcaataca gcaataaaaa
     2341 tgcgtcgtca gaactaccag actgtatccg caactcattg ttgtgagtgt ggcgatccga
     2401 tagatgagcg aagacgcctg gctgttcagg gttgtcggac ttgtgcaagt tgccaggagg
     2461 agatcgaact taagaacaaa caatggggac tgtgatggcc tcaaagcagc aaatttcaac
     2521 atcgtccaac tgaggtgtaa aaatgttcag aatcattttt cctaacacct ggtacgtcga
     2581 ccaccacggc actccctgca aaatcctgcg ttctacccac aacaaagttc actacatccg
     2641 aaaaggcaga acatgtatcg ccagcatgtt ccgctttaat catgactttg aacctgtgaa
     2701 taaagctgat gcagatcgga tagcagaaga gatcgaaacg gcagaacaca ttaagaagtt
     2761 acgtgacatg cgttcaaaaa gcagaggtaa ccatggaatc atacagcctc acactcgatg
     2821 aggcctgtca gtttcttaag atatccagac caaccgccac caactggata cgaacaggcc
     2881 gcctacaggc aacacgtaaa gatccaacca agccaaaatc tccttacctc acaacacggc
     2941 aagcctgcat tgcggcgctt cagtctccgc tgcatactgt ccaggtgagc gcgggtgatg
     3001 gcataacaga ggaaagaaaa tgtcactctt ccgcagaaat gaaatatggt atgcctcgta
     3061 ttcgctcccg ggcgggaaac gaattaagga atctcttggc acaaaggaca agcggcaagc
     3121 tcaggagttg cacgacaagc gaaaagcaga actctggcga gtagaaaagc taggggattt
     3181 acctgatgtc acttttgaag aggcctgcct aagatggctt gaggaaaaag ctgataaaaa
     3241 atctctcgat tcagataaaa gccggattga gttctggctt gaacattttg agggtataag
     3301 gcttaaagat atctcggagg caaagattta ctctgctgta agcagaatgc ataacagaaa
     3361 gacgaaagaa atatggaaac agaaagttca ggccgccatc aggaaaggta aagaaccgcc
     3421 tgtttatgaa ccaaagccag tatcaactca gacaaaggca aagcatcttg ccatgataaa
     3481 ggccattctc cgtgctgcag aacgcgactg gaagtggctg gaaaaagcgc ctgtcatcaa
     3541 gataccagcg gtcagaaaca agcgagtcag atggctggaa aaggaggaag caaaacgcct
     3601 tattgatgag tgccccgaac cactgaaatc tgtcgtcaag tttgcgctgg caactggtct
     3661 gagaaagtcg aacatcataa atctggaatg gcaacaaatc gacatgcagc gacgagttgc
     3721 ctgggtgaat ccagaagaga gcaaatcaaa ccgcgccatt ggtgtggcgc tgaacgatac
     3781 cgcctgtaaa gtgttgcgtg atcaaatagg caagcatcac aaatgggtgt ttgtacatac
     3841 caaggcggct aagcgagcag atggaacatc aacgcctgcg gtcaggaaga tgcgcatcga
     3901 cagcaagaca tcatggctat cagcttgtcg tcgtgcagga attgaagatt tccgtttcca
     3961 tgacctcaga cacacctggg caagctggct gattcagtca ggcgtcccat tatcagtgct
     4021 tcaggaaatg ggcggatggg agtccataga aatggttcgt aggtatgctc accttgcgcc
     4081 taatcatttg acagagcatg cgaggaaaat agacgacatt tttggtgata atgtcccaaa
     4141 tatgtcccac tctggaatta tggaggatat aaagaaggcg taactgattg aattgtaatg
     4201 gcgcgccctg caggattcga acctgcggcc cacgacttag aaggtcgttg ctctatccaa
     4261 ctgagctaag ggcgcgttga taccgcaatg cggtgtaatc gcgtgaatta tacggtcaac
     4321 ccttgctgag tcaatggctt ttgatctggt tgctgaacaa gtgaacgacc gcgtctgatt
     4381 ttctgattta tttcgctata gcggcaaaca aacacacacc gctgcgcgtc tgaatcaaga
     4441 aaacccgtat tttcatgtat caaagtacaa tttaccgccc taacggaaaa ttgtccgctc
     4501 ctatgagact ggtaactatg aaaccaacgt cggtgatcat tatggatact catcctatca
     4561 tcagaatgtc tattgaagtt ctgttgcaaa aaaacagtga attgcagatt gtcctgaaaa
     4621 cggatgatta ccgcataacc atcgattatc tccgaacccg tcctgttgat ttaatcatta
     4681 tggatataga cttgcccgga acagacggtt ttaccttcct gaaaaggatc aaacaaatcc
     4741 agagcacagt gaaagtgtta tttttatcat cgaaatcaga atgcttttat gctggcagag
     4801 cgatacaagc tggtgctaac ggttttgtca gtaaatgcaa tgatcagaat gatatttttc
     4861 atgccgttca gatgatcctc tccggataca cgttttttcc cagcgaaacg cttaactata
     4921 taaaaagcaa taaatgtagt acgaatagtt caacggtcac tgtgctatct aatcgtgaag
     4981 tgaccatatt acgttatctg gttagcggat tatctaataa agaaattgcc gataagttat
     5041 tacttagcaa taaaacagtt agtgcgcata aatctaatat ttatggcaag ctaggtttgc
     5101 at
//
LOCUS       DAZFWL020000229         5066 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFWL020000229 DAZFWL020000000
VERSION     DAZFWL020000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.230"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            122..838
                     /gene="nanC"
                     /locus_tag="ACHKNP_RS24035"
                     /old_locus_tag="pgaptmp_004807"
     CDS             122..838
                     /gene="nanC"
                     /locus_tag="ACHKNP_RS24035"
                     /old_locus_tag="pgaptmp_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HGU4706812.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVDVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            858..1964
                     /gene="nanM"
                     /locus_tag="ACHKNP_RS24040"
                     /old_locus_tag="pgaptmp_004808"
     CDS             858..1964
                     /gene="nanM"
                     /locus_tag="ACHKNP_RS24040"
                     /old_locus_tag="pgaptmp_004808"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGU4706813.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWKALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2029..3009
                     /gene="nanS"
                     /locus_tag="ACHKNP_RS24045"
                     /old_locus_tag="pgaptmp_004809"
     CDS             2029..3009
                     /gene="nanS"
                     /locus_tag="ACHKNP_RS24045"
                     /old_locus_tag="pgaptmp_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4706814.1"
                     /translation="MNAIISPDYYYILTIAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDMIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDKFVEAILQFWRER"
     gene            complement(3017..3667)
                     /locus_tag="ACHKNP_RS24050"
                     /old_locus_tag="pgaptmp_004810"
     CDS             complement(3017..3667)
                     /locus_tag="ACHKNP_RS24050"
                     /old_locus_tag="pgaptmp_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4706815.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
     gene            3803..3946
                     /locus_tag="ACHKNP_RS24055"
                     /old_locus_tag="pgaptmp_004811"
                     /pseudo
     CDS             3803..3946
                     /locus_tag="ACHKNP_RS24055"
                     /old_locus_tag="pgaptmp_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(4610..4738)
                     /locus_tag="ACHKNP_RS24060"
                     /old_locus_tag="pgaptmp_004812"
                     /pseudo
     CDS             complement(4610..4738)
                     /locus_tag="ACHKNP_RS24060"
                     /old_locus_tag="pgaptmp_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(4714..4863)
                     /locus_tag="ACHKNP_RS24065"
                     /old_locus_tag="pgaptmp_004813"
                     /pseudo
     CDS             complement(4714..4863)
                     /locus_tag="ACHKNP_RS24065"
                     /old_locus_tag="pgaptmp_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            complement(4948..>5066)
                     /locus_tag="ACHKNP_RS24070"
                     /old_locus_tag="pgaptmp_004814"
     CDS             complement(4948..>5066)
                     /locus_tag="ACHKNP_RS24070"
                     /old_locus_tag="pgaptmp_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4706816.1"
                     /translation="IFDNNEDKTDSAALLPCIEQAREDIRTLRQLQLQHQNR"
BASE COUNT         1465 a         1100 c         1182 g         1319 t
ORIGIN      
        1 aatacagcta ccggttgcca aagacactat aagcctggca aaaaaatatt acacaacata
       61 aatgctaatt gtttatgcgg gctttgtatt gctttctgtt tcctacaaat gagtgaaatt
      121 tatgaaaaag gctaaaatac tttctggcgt attattactg tgcttttcgt caccattaat
      181 ttctcaagct gcgacactgg acgtacgtgg tggatatcgt agtggaagcc acgcctatga
      241 gactcgactc aaagtcagtg agggatggca aaatggatgg tgggcaagta tggaaagtaa
      301 tacctggaat accattcatg ataataaaaa ggaaaatgcc gcactcaatg atgttcaggt
      361 tgatgttaat tacgcgatta aacttgatga tcaatggacg gtgcgcccgg gaatgttaac
      421 gcattttagc agcaacggca cacgctacgg accctacgta aaactgtcct gggacgcgac
      481 aaaagatctt aattttggca ttcgctatcg ttacgactgg aaagcttacc gacaacaaga
      541 cttatccggt gatatgtctc gtgataacgt tcatcgttgg gatggatatg tcacttacca
      601 tattaatagt gatttcacct tcgcatggca aacgacgcta tacagcaaac agaacgatta
      661 tcgctatgca aaccataaga aatgggcgac ggaaaatgca tttgttctac aataccatat
      721 gacgccggat attacgccat acatagaata tgactacctt gaccgtcagg gtgtttacaa
      781 cggcagagat aatttatcgg aaaacagtta tcgcattggt gtgtcattta aactgtagta
      841 gacaggagac agtcacaatg aataaaacaa taacggcgct tgctatcatg atggcttcat
      901 ttgccgcaaa cgcgtctgta ttaccggaaa ctcctgtgcc atttaaaagt ggtaccggag
      961 caattgataa cgacactgtc tacattggtt taggtagcgc aggtacggca tggtacaagc
     1021 tggatacaca ggccaaagat aaaaaatgga aagcgttagc tgcattccct ggtggaccaa
     1081 gagatcaagc aacctcggca tttattgatg gcaatctgta tgtgtttggc ggcattggca
     1141 aaaacagcga gggcttgact caggtattta atgacgtaca caaatacaac cccaaaacca
     1201 atagttgggt taaattgatg tcgcacgcac cgatgggcat ggcgggtcat gtaacttttg
     1261 tacacaacgg caaggcttat gttactggcg gtgttaacca gaatatcttc aatggctatt
     1321 ttgaagatct caacgaggct ggaaaagatt caaccgctat agataaaatc aacgctcact
     1381 attttgacaa aaaagcagaa gattattttt tcaataagtt tctgttgtct tttgatccct
     1441 caacacagca atggagttac gctggcgaat cgccctggta cggaacggct ggtgcggcgg
     1501 ttgtgaataa aggtgataaa acctggctta ttaatggcga agccaaacca ggattgcgaa
     1561 cggatgccgt atttgaactt gatttcaccg gtaataattt aaaatggaat aagcttgctc
     1621 ccgtctcatc accagatggc gtagctggcg gttttgcggg gataagcaat gattctctta
     1681 tatttgccgg aggggccgga ttcaaaggtt cacgagaaaa ttaccagaac ggtaagaact
     1741 atgcgcatga aggcctgaaa aaatcatata gcactgatat tcatctttgg cataacggga
     1801 aatgggataa atcgggtgaa ttatcgcaag gtcgggccta cggagtatca ttgccctgga
     1861 ataatagtct attgattatt ggcggtgaaa ctgcaggcgg caaagcggtg acggattcag
     1921 ttttgatctc tgtgaaggat aataaagtta cagtacaaaa ctaacgcttc agggccccgg
     1981 taaaggggcc ctgctatcaa tttgtcattt caattacgcg aaatttatat gaacgcaata
     2041 atatcgcccg attattacta tattcttacc attgctggtc agtctaatgc catggcgtat
     2101 ggcgaaggac tgccattacc ggacagggaa gatgcgcctc atcccagaat caaacaatta
     2161 gcgagatttg cgcatacgca tcccggaggc ccgccatgtc actttaacga catgattcca
     2221 ctgactcact gcccacacga tgttcaggat atgcagggtt atcaccatcc tctggcaacg
     2281 aatcatcaaa cacagtacgg caccgttggc caggcactgc atatagcacg gaaattactg
     2341 ccctttattc ctgataatgc aggggttctc atcgttccgt gttgccgtgg cggatcggct
     2401 tttaccgcgg gcagcgaagg gacatattca gaacggcacg gagccagcca tgatgcttgt
     2461 cgttggggaa cggatactcc gctataccag gatttagtca gcagaacgcg agccgcactg
     2521 gcaaaaaatc cgcataacaa attcctcggc gtatgctgga tgcaaggcga atttgactta
     2581 atgaccagtg actacgcgtc acaccctcaa cactttaatc atatggttga agcctttcgt
     2641 agggatctaa aacaatacca ttctcagctt aataatatta ctgacgcacc gtggttttgc
     2701 ggcgatacca cctggtactg gaaagaaaat ttccctcatg cgtatgaagt tatttatggc
     2761 aattatcaaa ataatgtttt agccaatatt attttcgtcg acttccagca acaaggtgaa
     2821 agaggactga cgaacgcgcc tgatgaagac ccggacgatt taagcacggg atattacggt
     2881 tcagcgtacc ggtcaccgga gaactggacg acggcactgc gaagcagtca tttcagcacg
     2941 gcagcccgtc gggggattat ttctgacaag tttgtagaag caattttgca gttttggcgc
     3001 gaaaggtgag cgttcattaa tttgatttat tcgcatccat cagcgctttt aactgctccg
     3061 gcgtaaacgg tggtttgcgt cggtgcaaca tcgcatggca attagggtac acaggcagca
     3121 aatcattaac tggatccagc tggtaatcct ctttaatggc tgacagcggc accaggtgat
     3181 gcacatggat aaagccttca gcgatatcac catagatctt caccagatca acaccgcata
     3241 cgttgcactg gcatccatga tgttcgaccg ctttatctcg ggccttttta tcgcgttcat
     3301 agcggtttac tgtcaccttt atcgccgcac cttcaaggta tgtctcctgc gacggcaact
     3361 catccgggaa cagaacgtcg gctatgggag ccgcagcggc tttatccaga tgaatatgaa
     3421 atccctgctc tatgagtagc ttgacacatt tagattttat tccgccggtg aaatccgcag
     3481 gggtaaattg cgtccccgtc atcaatgtcg ctgcgatgcc agcaatcgcc ttcgaggggt
     3541 aacgtcggcc atgatattca atctcataca accgcgcagg ctggaactga tgagtttctc
     3601 ccgcatccca taaatggata gcttcgatga tataccgccg cggaagatga tcagggagtt
     3661 tattcatgga gaatgtcctt atacgttctc agaacaggca gcaaaaaaca aatgagatca
     3721 aggtaatata tcttcacttg caggcttgaa cagcagaaaa tttgcgagca tgatcgcaat
     3781 tatttcactg ccagggatat caatagcttc cagtaaatcg ctacagcagg caattgccaa
     3841 tataaaaatc tggcacaagg gtgaacagcg cgcgccgcat aagccattgt tattgctata
     3901 cgtattagcg ggatacctga atggacatcc gcgccttttc gattattgta gtggaacagt
     3961 ctacgaggat gcagttcgaa gtgagtaagt aaaggttgcg ggaaacgatc aatgccgcca
     4021 tacaatgatt ttttaggatc aacaatgagt aataaaaact acgaaagtca ccgcaaagcg
     4081 attgttagta aaggtatacc acccgctcta ttaaataggc tcaccaattc ggatgttcag
     4141 gtgatcaata ccttcttaac acgagtgagc aaactggaac tatctcaaca agagaaagac
     4201 tggatcataa aaatcatctc tatggtttag acaatttcag ccttgtagcc tttcaagggt
     4261 tttgtgtggg tacaaacatt atcgaatcgg ggtatagccg cagctgtagc tgtatccaca
     4321 tcagtaagtt gatgtaggtt gacttaggtt gagcgaaaaa tagcgaaaag ccttgtgtgg
     4381 cgcggatttc agtcataaaa aaacgtccgt tgatgtctat tgatgttccg atggtgcgaa
     4441 ggtgcgaagg tgcgaaggcc ggactcaaac atgtgactta acctcatgat ttaaaattgt
     4501 taataataaa caatatcatc ttataccaac acagatacca acacgaaaaa tcactggttt
     4561 tcggcatcga accaggacaa tcagctcctg attgagatac cgagctctgt tactcgtcat
     4621 cttcctcgac aggcttcatt gcatcaagta gtactcgcac catcgcgata gaaccaatcc
     4681 ggataaaagc atcttctcca tctatatctg ttaactgata tcgctcactt gatgccggat
     4741 aattgtgtcg atttgcagca gtgaactctt tcgtacagcc agaatgcggc ggatgctggt
     4801 tgagcagata acgtccgtat tctcgttgac cacttccgga cggtaggctg tgatatcaga
     4861 cataatcgat attccttttt gcagataaat gaaaatgcca gccctgttca ggctggcgtc
     4921 agtggaatga agtctggtga gtaagactca ccggttctgg tgctgaagct gcagttggcg
     4981 cagggtacgg atatcctctc tggcctgttc gatacagggc aacagtgctg ctgaatccgt
     5041 tttatcctca ttgttgtcaa agataa
//
LOCUS       DAZFWL020000230         5055 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFWL020000230 DAZFWL020000000
VERSION     DAZFWL020000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.231"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(465..680)
                     /locus_tag="ACHKNP_RS24075"
                     /old_locus_tag="pgaptmp_004815"
     CDS             complement(465..680)
                     /locus_tag="ACHKNP_RS24075"
                     /old_locus_tag="pgaptmp_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706817.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPIDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
     gene            complement(767..2143)
                     /locus_tag="ACHKNP_RS24080"
                     /old_locus_tag="pgaptmp_004816"
     CDS             complement(767..2143)
                     /locus_tag="ACHKNP_RS24080"
                     /old_locus_tag="pgaptmp_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU4706818.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDTSSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERNKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGPLGTVYQRFCNGHFVACDQDEARQICTASNAPASRGR
                     RYAQGADV"
     gene            complement(2140..2961)
                     /locus_tag="ACHKNP_RS24085"
                     /old_locus_tag="pgaptmp_004817"
     CDS             complement(2140..2961)
                     /locus_tag="ACHKNP_RS24085"
                     /old_locus_tag="pgaptmp_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU4706819.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEIIDSLLMADLT
                     VRQMKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAESANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            complement(2948..3109)
                     /locus_tag="ACHKNP_RS24090"
                     /old_locus_tag="pgaptmp_004818"
     CDS             complement(2948..3109)
                     /locus_tag="ACHKNP_RS24090"
                     /old_locus_tag="pgaptmp_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706820.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            complement(3145..3423)
                     /locus_tag="ACHKNP_RS24095"
                     /old_locus_tag="pgaptmp_004819"
     CDS             complement(3145..3423)
                     /locus_tag="ACHKNP_RS24095"
                     /old_locus_tag="pgaptmp_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HGU4706821.1"
                     /translation="MELTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMAMLLAVLEWGVEDEELAELAKKVAMVLTKEKPQDCGNSFEA"
     gene            complement(3533..3757)
                     /locus_tag="ACHKNP_RS24100"
                     /old_locus_tag="pgaptmp_004820"
     CDS             complement(3533..3757)
                     /locus_tag="ACHKNP_RS24100"
                     /old_locus_tag="pgaptmp_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4706822.1"
                     /translation="MNLVIQRALKIVGSQKRLADKCGVTQPAVHKWLKGGLVSPEKVT
                     AIVNATGGQIKAYEIRPDLPHLFPKPNQAA"
     gene            3869..4576
                     /locus_tag="ACHKNP_RS24105"
                     /old_locus_tag="pgaptmp_004821"
     CDS             3869..4576
                     /locus_tag="ACHKNP_RS24105"
                     /old_locus_tag="pgaptmp_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4706823.1"
                     /translation="MKTFAERLNAAMSSAGVSQSQLADMVGISQPAIQKMSSGKTNGS
                     RKMVELANALKVRPEWLSSGIGEMRDGAHEEPSNVRDSSLKAVVWEDIKRNDDEFVAL
                     PLLNVSLSAGSGSCELEESSEFSLVFRKHYLKKMGVSERSAKLVRVVGQSMEPTLHDG
                     DVVGVNTQDTTIRDGKTYAICQSDLLRVKTLIATPTSVIIRSINREEYPDEVMDRDEF
                     HETVRIIGRVFWSSHSW"
     gene            4617..4913
                     /locus_tag="ACHKNP_RS24110"
                     /old_locus_tag="pgaptmp_004822"
     CDS             4617..4913
                     /locus_tag="ACHKNP_RS24110"
                     /old_locus_tag="pgaptmp_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706824.1"
                     /translation="MEFLIVFVVVLVIILFVLLSISKKLSQMIEHSSNRAKEEEHIID
                     IKEILSDIKITLDEIKYTTDLIEQYKIPTPNERKAIDQYRIDLEIDEMLSKRKD"
BASE COUNT         1313 a         1264 c         1086 g         1392 t
ORIGIN      
        1 tcactctcca tcgatgattt tttgggttac caataatatt tgatagtcgc cataattatc
       61 ggtagcaacg cgcagacaac tgaaaaccgt aaaacaaaac ctactcccaa tatgcgatat
      121 ccattattcc agagaacaaa actcatcatc agaaggaatc catgaaaaat cgcgacaaga
      181 aataaactac aaataaatgc atttaccatc ggtacttacc cctcgctctt aatccaataa
      241 aaaagggttt gcttcactga acactccttt attttttatg cctgtaaccc cattcttcca
      301 gcaaccttgc ggcgtaccac ccaagaaaca aaggaaagaa cattacaatg agatattccc
      361 cgccacggtc aatgttcgaa attgaccaga ttacgatgta accagtgcag gacaggaata
      421 ttacaaaccc caaaaagcta cttcttcgac tcatgctcac tccttcactt taaatccaga
      481 ctccggatta ttctgttgcg ctgaaactca ttgttgagtt tgaacaaccg tcgaaggaca
      541 cggtcacgcg gatagcgtcg tgcagcaggt gaatgctcat acaactcatc aatcggcaaa
      601 ctggacgatg aacgataccg ataccaacgc accaactctt cacgaaaatt agccctgaca
      661 agctcagcta tcgtactcat ttcttaaagc ctccaattcc ctctccccca aataaaaagg
      721 cctgcgatta ccagcaggcc tgttattagc tcagtgatgt agatggtcat acgtcagccc
      781 cttgtgcata tcgtctgcca cgcgaagcag gtgcatttga tgctgtgcaa atctgtctgg
      841 cttcatcctg gtcacatgca acaaagtgtc cgttacagaa ccgctggtaa accgtaccaa
      901 gtgggccaaa ccggtttttc gtcacaatga tttcagcaaa tggtgcggcg ctactgttct
      961 cgtcatatac cgcttcccga tagagcatga tgattgagtc tgcgtcctgc tcaatgcttc
     1021 ctgaatcacg caaatctgcg tttgtcgggc gtttgtttgg tcgcttctca acatcgcgcg
     1081 aaagctgact tagggagata acaggcgttt tcaggtcttt cgccatcgcc ttcaggcttc
     1141 ctgagatgtg agcaattgcg aggtcgttgc ggtctgcttt cggcttctca atcaggccaa
     1201 gataatccgc catgatgagt gacaggtttg gattttcctg tttgttccgc tctgcgattg
     1261 agcgaatttc ttctaccgat aaccgcgagg catcgactac ccatacatcc aaatctgcaa
     1321 gctgactcat gccgttagca acacgcgccc agccttcgtc atccatcgat gcaggatttc
     1381 gcagtacgct aaccgacatc ctcccggcgt tggcaatgct ccgctctgca atctgcaatg
     1441 cgctcatttc catcgagaaa atcaacactc cgcgccggac gtcagaacca ggaataacgc
     1501 ggcttgcaac accttcggca atcttcagcg ccagttcggt tttccccata ccaggacgag
     1561 cggcgataat caccaggtct tccgcgttca tccctccggt gatggcgtca agttcttcga
     1621 ttccggtctt cagggtatct gactcttctc cgttcctcag acgcctgtca agcgtgtcag
     1681 tgtagtcagt gatgatttcc cctaaccgta caggtttaac ctcgtcacgg ggctttctga
     1741 tggctgagag acgctttaca agctcgtcca tcgcctgact cgatgtatcg atggttccgc
     1801 tctgaattgg ttcacgcatt tcgtccatga tttccagcac cagacggcgg tgatagttat
     1861 ccgcgaccat tccggcatat cccttcaggt ttgcggcact cgggcagttt ttgctggtca
     1921 tcaggattga cgtgaaatgc tcctctccgc acgcctcggc aaccatcagc gcgtcgatta
     1981 ggtttctgtt tctcgcctgc ttacggataa cttcgaaggc tttccggtag agcggaattg
     2041 aaaacgcttc cggctccagc gttgccagaa cgtcgctggc ggttggtgtt aatccaccaa
     2101 tcagcaagcc accgataacg ctcgcttcga tatcctgtct catgcaatcc ccctgtctgc
     2161 aaacttccct tcccgaactc ccgttaacga gtcttccctc agcaggtaat caaaatctgc
     2221 cgtccagccc gtgtcgttgt ctccgaagta aaacggcttg gcctgatgca caaacgccct
     2281 gacatacgct ctgaaaccgt ccacgtttgg cgttttcagt tgcgggatga ttttcttcag
     2341 gcggcgtttt cgtttctcgt tgaccgcaac agcgtgtgga agtctgtcac cgacttcggt
     2401 gttgtaggcg ttcaggaagg attcgtagtc gattcgttct gccttgcgac gttcaggttt
     2461 aacctgccca tcgccgcccc cgttaggggg taagggggta tttgtattta ttgtcttttg
     2521 tatattgtct tttgtgttta gctgacttgg cttataccca ttagccgact cggctaatgt
     2581 tttattagct gttttagcta atgttaagct gtcctggcta atccactgag aaaccacctt
     2641 gttcactccg attttcacgc catcagcaat gaggaattta cgctcaataa gctggcgctt
     2701 ggcagcgcaa acatgagtgt gatgaatacc tgtcatggct gctatctgcg tgtttgtgag
     2761 tcgatccatc ggcttattga atccgtatgt cttgcgcatg atagcgagca tcaccttcat
     2821 ctgccggacg gttaaatcag ccatcagcag actgtcgata atctcgttag caacgcgcat
     2881 gaaaccatct tcggtatctg ccacgcgatg ctccacgacc tccagttgag gcctgtaatc
     2941 agctaactta acgacgccca tgtttcactc ctgctttggc tagtctgtaa acaccaacaa
     3001 ggcgctctgc gaacgccctg ttatttgctg cggctaccac taatccctca ggtgaatcag
     3061 ggtgtcgaat ctcttctttt tcctggtatt tcttacgacg ttttgtcata attactcctg
     3121 tggattgata cagtctttct acaatcaggc ctcaaaactg ttgccgcagt cttgaggctt
     3181 ttcttttgtc agcaccatgg ctactttctt tgccagctcc gctaattcct cgtcttcaac
     3241 accccactcc agcacagcca gaagcatggc catctttggg ataaagctgt ctttccatcg
     3301 cgaaatttgc gattcattga tccctaatgc atcagcaacc tttcgctgac cacgtacagc
     3361 aattcgattc aggatgttgc ttgtaattgc attcgctttc ttgcgagtac ttgtaagttc
     3421 catatgtaag tatttcctta acaaataaga agttatgcgc atcaacttat gcgcgttgta
     3481 ttcccgcatt tcggcgggaa tgaggaccat gactgttaaa gagcggtgtt acttatgctg
     3541 cctgattcgg ttttggaaac aggtgtggca aatcggggcg aatttcgtaa gccttgatct
     3601 gccctccagt ggcgttaacg atggcggtaa ctttctctgg agagaccaac ccgcctttca
     3661 gccatttgtg tactgctggc tgcgttacac cacacttgtc ggcaaggcgc ttttggctac
     3721 cgacaatttt caaggctcgt tgaattacta aattcatgag catacctctt gtggtcatta
     3781 cttataacca aagataactc aagttataaa aaacagcaat aacctttgtt attttacttt
     3841 ggataaccgt agttatagat ttgtgggtat gaaaacattc gcagaaagac taaatgcagc
     3901 catgagctca gcaggggtat cacaatcaca gcttgctgac atggttggaa tatctcagcc
     3961 agccatacag aagatgtcgt ccggtaaaac aaacggatct cgcaagatgg ttgaattagc
     4021 caatgcttta aaagtgcgcc ctgaatggct tagttctggt attggtgaaa tgagggatgg
     4081 tgcacatgaa gaaccatcca atgtccgtga ttcatcttta aaagctgtgg tatgggaaga
     4141 cattaaaaga aacgatgacg agtttgttgc gttgcctctt cttaacgttt cgctttcagc
     4201 tggaagcggt agctgcgagc tagaggaatc atcggagttc tctttggttt tcagaaagca
     4261 ctatctgaaa aagatgggag tatctgaaag atcagccaag ctagttaggg ttgtagggca
     4321 aagcatggaa ccaacgcttc acgatggcga tgttgttggt gttaacacgc aagataccac
     4381 aatcagagat ggtaaaacct acgctatttg ccagtctgat ttgttacgag taaaaacatt
     4441 aatcgccacc cctacatcgg tgataatcag atcaataaat cgcgaagagt acccggatga
     4501 agtaatggat agagatgaat ttcatgaaac cgtaaggatt attggcagag tattctggtc
     4561 gtctcatagt tggtaaccga taatcagaag aagacttacg gaagtgcgga gggataatgg
     4621 aatttctgat agtttttgtt gttgttttgg tcatcattct ttttgttttg ctaagcatta
     4681 gtaaaaagct atctcaaatg attgaacata gctctaatcg tgcaaaagaa gaagagcata
     4741 taattgatat aaaagagatt ctctctgata taaaaatcac attagatgaa ataaaataca
     4801 caacagatct aattgaacag tataaaatac caaccccaaa cgagagaaaa gcaatagatc
     4861 aatatcgtat tgacttagaa atcgacgaaa tgctaagcaa aagaaaagac taaaaacacc
     4921 cggcctcagc gccgggtttt cttttcctgc cgttcaccac ccaatcaacc atcctcatca
     4981 tagacaagca tcaagccaaa ggtaacacct tcaccccgca cgcaagccct caaacaccaa
     5041 tcaatcagca acatt
//
LOCUS       DAZFWL020000231         5002 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFWL020000231 DAZFWL020000000
VERSION     DAZFWL020000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.232"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..939
                     /locus_tag="ACHKNP_RS24115"
                     /old_locus_tag="pgaptmp_004823"
     CDS             <1..939
                     /locus_tag="ACHKNP_RS24115"
                     /old_locus_tag="pgaptmp_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HGU4706825.1"
                     /translation="QIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNA
                     AKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGAL
                     DIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQT
                     DLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQN
                     FSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLP
                     FPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1148..2113
                     /gene="lipA"
                     /locus_tag="ACHKNP_RS24120"
                     /old_locus_tag="pgaptmp_004824"
     CDS             1148..2113
                     /gene="lipA"
                     /locus_tag="ACHKNP_RS24120"
                     /old_locus_tag="pgaptmp_004824"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HGU4706826.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2214..2417)
                     /gene="tatE"
                     /locus_tag="ACHKNP_RS24125"
                     /old_locus_tag="pgaptmp_004825"
     CDS             complement(2214..2417)
                     /gene="tatE"
                     /locus_tag="ACHKNP_RS24125"
                     /old_locus_tag="pgaptmp_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HGU4706827.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2546..3334)
                     /locus_tag="ACHKNP_RS24130"
                     /old_locus_tag="pgaptmp_004826"
     CDS             complement(2546..3334)
                     /locus_tag="ACHKNP_RS24130"
                     /old_locus_tag="pgaptmp_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HGU4706828.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3427..3810
                     /gene="crcB"
                     /locus_tag="ACHKNP_RS24135"
                     /old_locus_tag="pgaptmp_004827"
     CDS             3427..3810
                     /gene="crcB"
                     /locus_tag="ACHKNP_RS24135"
                     /old_locus_tag="pgaptmp_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGU4706829.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3865..4074)
                     /gene="cspE"
                     /locus_tag="ACHKNP_RS24140"
                     /old_locus_tag="pgaptmp_004828"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3865..4074)
                     /gene="cspE"
                     /locus_tag="ACHKNP_RS24140"
                     /old_locus_tag="pgaptmp_004828"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU4706830.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4249..4809)
                     /gene="pagP"
                     /locus_tag="ACHKNP_RS24145"
                     /old_locus_tag="pgaptmp_004829"
     CDS             complement(4249..4809)
                     /gene="pagP"
                     /locus_tag="ACHKNP_RS24145"
                     /old_locus_tag="pgaptmp_004829"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HGU4706831.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1340 a         1258 c         1113 g         1291 t
ORIGIN      
        1 caaattgaac cctgtttaag cagaaagtcg tcagaaggta aaccacaaat atttacaacc
       61 ttacgaaata ttgatcttaa ccttctgact atttttgaag ctgtatatgt acataaaggg
      121 atcgttaatg cagcgaaagt gcttaatctg accccctcgg caatcagtca gtctattcag
      181 aaactgcgcg ttatattccc tgacccattg tttattcgca aaggccaggg tgtcactcct
      241 accgcatttg cgatgcatct acatgagtat atcagtcagg gccttgagtc cattcttggc
      301 gcgctggata tcgaaggaag ctatgataag caacgaacga taactattgc gaccactccc
      361 tcggtcggag ccctggtcct tcctgtcatc tatcgggcga ttaaaactca ctatccgcag
      421 cttttactgc gcaacccgcc catcagcgac gcggaaaacc aactcagtca gtttcaaacc
      481 gatctcatca tcgataacat gttttgcacc aatcgtacgg tgcaacatca tgttctgttc
      541 accgacaata tggtgttaat ttgccgcgag ggaaatccac tactctcttt agaagatgac
      601 agagagacta tcgacaacgc tgcgcatgta cttctgttac cggaagagca aaatttcagc
      661 ggtctgcggc agagagttca agaaatgttt ccggaccggc aaatcaattt caccagctac
      721 aacattttga caatcgctgc actggttgcc aacagtgaca tgttagcgat tattcccagc
      781 cgtttttata acctgtttag ccgctgctgg ccgctggaaa aactgccttt tccgtcctta
      841 aatgaggagc aaatagattt ctctatccac tacaacaaat tcagcctgcg tgatcccatc
      901 ctgcacggag taatagatgt tatccgtaat gcattttgaa aaagtattgt tacagccaga
      961 tcacagcgca gaacgataaa atggcacaat ccaacaacaa ttttacattt tcgcgaccgc
     1021 tttggctgct ttcaggtccg tttcaatgat atactgccag tcgttaattc aaaaatagtt
     1081 gataattaca acaatctatt gaattgaaac gctttccttc gtaattcgca actggaacac
     1141 gcacgctatg agtaaaccca ttgtgatgga acgcggtgtt aaataccgcg atgccgataa
     1201 gatggccctt atcccggtta aaaacgtggc aacagagcgc gaagccctgc tgcgcaagcc
     1261 ggaatggatg aaaatcaagc ttccagcgga ctctacacgt atccagggca tcaaagccgc
     1321 aatgcgcaaa aatggcctgc attctgtctg cgaggaagcc tcctgcccta acctggcgga
     1381 atgcttcaac cacggcacag caacgtttat gatcctcggc gctatttgta cccgccgttg
     1441 tccgttctgt gatgttgccc acggtcgccc ggtagctcct gatgccaatg aaccagtgaa
     1501 actggcgcag accattgccg atatggcgct gcgttatgtg gttatcacct ccgttgaccg
     1561 tgatgacctg cgcgatggcg gtgcccagca ctttgcggat tgcattactg ccattcggga
     1621 aaaaagcccg caaatcaaaa ttgaaactct ggtgccggat ttccgtggtc gtatggatcg
     1681 tgctctggat attctgactg caacgccacc agatgtgttc aaccataacc tggaaaacgt
     1741 accgcgtatt taccgtcagg tacggcctgg tgcagattac aactggtcgc tgaagctgct
     1801 ggaacgcttt aaagaagcgc atccggaaat cccgaccaag tctggtctga tggtgggact
     1861 gggtgaaacc aatgaagaaa ttattgaggt aatgcgcgac ctgcgccgtc atggtgtgac
     1921 gatgttaacg ctggggcaat atttgcagcc aagccgccat cacctgccgg ttcaacgtta
     1981 cgttagcccg gatgagttcg acgaaatgaa agccgaagcg ctggcgatgg gctttaccca
     2041 tgctgcatgc ggtccgtttg tccgctcttc ttaccacgcc gatttgcagg cgaaagggat
     2101 ggaagttaag taatcctgta ataattactt acatctgtct gataaaaaac ccgctttttg
     2161 aagcgggttt tttgtatcga acaagatatt gagggagcgt cctgctcgcc acgtcactct
     2221 ttatgagaga gcttttcagc ctgaagatca acgtctgcgc cttttttcgc cgcagcatcg
     2281 tcatcattca tcgccttctt gaacccttta atggccgctc caaggtctcc gcccagcgta
     2341 cgtaacttct tagtcccaaa cagcagaacg accagcgccg caactactag cagtttggta
     2401 atactaatct cacccataga taccttcttg acatataacc ggcaacagaa cgcgccttaa
     2461 aaaccagaga atattaacgg tcattcgacg cgcgcacaca atagcaatct gtaacaaggt
     2521 gaatcaagcg ccgagtaaaa aaacatcata ataattgcgg cggcgcaaag cgacggttgt
     2581 ttaagacggg cagttgcgcg cgcacctgac gcacacgttc gggcgtcact tccgccataa
     2641 tgagtgcagg catttctgac gctgccgcaa tggtgacgcc aaagggatct ataatccggc
     2701 tttgaccgat atttttgttc ccgcactccc ccgccgccac catataacag gtggtatcca
     2761 gcgcacgagc ggcaagcaac gttgaccaat gatgctcttt gagcggcccg cgaacccagg
     2821 cggcaggaag taccaggatt tcagctccct gtaatgcctg tgccagcgcc agctctggaa
     2881 agcgtaagtc ataacaggtc atcagaccga ccttcatccc ctccacctcg agtaacggag
     2941 cgatttcatt accagcatca acacggcgtg attcctgaat ggcaaatgca tcatagagat
     3001 gcaatttggc ataacgggcg acgatgtttc ctgcctgtag cgccaccagc atattccatg
     3061 cgcgccccgg cgttgaagga acatgaatcg tcagaattgt cgtcatcatg ttacgtttac
     3121 tttctcgccg taaacgtccg aggaattcgc cttccagcag ctgtgctgat ttaaccgata
     3181 gatctgcatc atgatcatcg cgcgccagca atgcttccgg caggacgaac agcgatacgt
     3241 cgttttccgc cgcctgcgcc atcaacgagg cacaaatctc agcgttcttt tcccacacag
     3301 atgtaacagc aaactgtcct gcggcaacca gcattgccac ctcctgtcag agtcgtaaaa
     3361 ttcgccaacg acgattattc ggcgttacac ttatcactca tacaaatcaa atagcaggat
     3421 tttgcagtgt tacaacttct tttagcagtt tttattggcg gtggtacggg aagcgtggcg
     3481 agatggctgt taagtatgcg atttaacccg ctgcatcagg cgattccgtt ggggacgctg
     3541 gcagcaaacc tgattggggc attcatcata ggaatgggat tcgcctggtt cagcaggatg
     3601 acgaacattg atccagtgtg gaaagtatta atcaccaccg gattttgtgg cggtctaaca
     3661 accttctcaa cattttcggc agaagtggtg tttttgttac aagagggccg ctttggctgg
     3721 gcgttactga acgttttcgt caaccttctg gggtcttttg ccatgacagc actggctttc
     3781 tggctgtttt cggcctcaac cgcacactaa agaccaaaaa aaaacccgct gattaagcgg
     3841 gttttgaatt cttgctgacg tatcttacag agcgattacg tttgcagcag aagggccttt
     3901 ggcaccgtta gtgatttcga actctacgcg ctgaccttca gcaagagttt taaaaccatt
     3961 agtctggatt gcagagaagt gtacgaacac gtctttgctg ccgtcttccg gagtaatgaa
     4021 accgaatcct ttggactcat taaaccactt aacgttacct ttaatcttag acatcaaaat
     4081 tacctttaca tgaaaaatag acacaaatgc tgtgtcggtt accagtacac caattgtggt
     4141 acgctttgtc cagtgcaact ttactaaaaa gtgataaaag tcgcgttatt tttgatgtta
     4201 cttattttag ctattgattt taaagaagtt actaaaactt catttgtctc aaaactgaaa
     4261 gcgcatccag gcaaagtaca cattgccatt gttgtaggta cccggaatgt aggtcatctg
     4321 aaaagtcact gggccataac ccacggaggc caatggcagt agaaccggga gagggatgta
     4381 attccagtta tcgcgtgccg ttacgccagc ggtgaatccc agacctaaat gaaaattttc
     4441 atccgccagc ggtcgccagg tactttccca tccgtatccg gcaatcggtt cccatttgtt
     4501 ccacgagtcc ttaaatgcca tggcatacag gccatgccag tttccttttt catcccaacg
     4561 cgacaggcca aaaccgccac cccacggtcg ctcgttatag cgatcggttt tttctttgtc
     4621 gtaagcgaaa cgtgcatgcc aggtgatggc aggaatatat aaatcataat gttcaggctg
     4681 ttgccaggtt tgtgcaatat tttctctaaa cgttgtcatc cactcatctg cgttagcaaa
     4741 aactttacca acgctgatta actgaataaa aacaaaggaa aagatagcga catatttact
     4801 cacgttcatt tgtgaccata aaacatttat caaaaatcta ctactagcat agcaaagcta
     4861 caattaacat aaccttaata gacccaacat aaagaataat ctgaatagct gctctattta
     4921 tttccgcttg ttcaagttaa atatttccgc gcatcgatta aagatgatgc gtcgttttct
     4981 gcgatgggaa tagtcaaaaa ag
//
LOCUS       DAZFWL020000232         4989 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFWL020000232 DAZFWL020000000
VERSION     DAZFWL020000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.233"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..168
                     /locus_tag="ACHKNP_RS24150"
                     /old_locus_tag="pgaptmp_004830"
     CDS             <1..168
                     /locus_tag="ACHKNP_RS24150"
                     /old_locus_tag="pgaptmp_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HGU4706832.1"
                     /translation="HGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            611..4513
                     /gene="espP"
                     /locus_tag="ACHKNP_RS24155"
                     /old_locus_tag="pgaptmp_004831"
     CDS             611..4513
                     /gene="espP"
                     /locus_tag="ACHKNP_RS24155"
                     /old_locus_tag="pgaptmp_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HGU4706833.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVIARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4761..>4989)
                     /locus_tag="ACHKNP_RS24160"
                     /old_locus_tag="pgaptmp_004832"
     CDS             complement(4761..>4989)
                     /locus_tag="ACHKNP_RS24160"
                     /old_locus_tag="pgaptmp_004832"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706834.1"
                     /translation="AQNQPAETVRAAAAATGRFAVAFPVRVAPGSGCFMKTNAPRLFF
                     VDKESGVSFDVFHVLSVSFPVTACATHDGGM"
BASE COUNT         1515 a         1132 c         1112 g         1230 t
ORIGIN      
        1 catggtggtg agcggggagc gctactgtac agcctgatcg ggacgtgcaa actgaatgac
       61 gtggatccag aaagctacct tcgccatgtg cctggcgtca tagcagactg gccggtcaac
      121 cgggtcagcg aactgcttcc gtggcgcata gcactgccag ctgaataaca catccccgtc
      181 aatacggccc tcgctgtacg cttacgcata aatattgaat acatttactg agcatgcaaa
      241 agaaaaagac aaactaatcc cggcctttca cagaatttcc tctatcagag gctgagttga
      301 caacctgctc acccaggaaa tagtaacacc cgaaaacatg aagagactct gataaacaat
      361 ctcgatacat attcctcatc tttcacagca tctcacttct ttaacaggaa ttattattat
      421 ctacgcctcc tcctttcatg tatccctcat gaatgattaa gataaacata ctgtgtttgt
      481 tatccgtatg ataacaaaca cataaaggag taaacattta tctggatgat gattacaatg
      541 ttcacgactg tcagagacac acctctgata cattagaaaa accaatgttt cccttaaaaa
      601 tggagcttat atgaataaaa tatactctct taaatacagc catattacag gagggttaat
      661 cgctgtttct gaattatccg gcagagtatc atcaagagca actggtaaga aaaaacacaa
      721 acgcatactt gcattatgtt ttttaggctt attacaatcc tcatattctt ttgcgtcaca
      781 gatggatatt tcaaatttct acatccgtga ctatatggat tttgcacaga acaagggcat
      841 atttcaggct ggcgcaacaa atattgaaat agtgaagaaa gatggctcca ccctgaaact
      901 accggaagta ccatttcctg acttctcacc ggttgcaaac aaagggtcaa ccacatctat
      961 tggtggtgca tacagtatca cagccacaca caatacgaaa aaccaccact cagttgcgac
     1021 gcaaaactgg gggaacagca cgtacaaaca aactgactgg aatacttcac atcctgattt
     1081 tgcagtatcc cgacttgaca agtttgttgt tgagacccga ggtgcgactg aaggcgcaga
     1141 tatttcgtta tcaaaacagc aggcacttga acgttacggg gttaattata aaggagaaaa
     1201 gaaacttatc gcattcagag ccggctctgg tgtggtatcc gttaaaaaaa atggacgcat
     1261 aactccattt aatgaggttt cttataagcc agaaatgtta aatggctctt tcgttcacat
     1321 tgatgactgg agtggatggc tgatattaac caacaaccag tttgatgagt ttaataacat
     1381 tgcctctcag ggtgacagcg gttcagcact gttcgtctat gataaccaaa agaaaaagtg
     1441 ggttgtcgct ggaactgtct gggggattta taattacgcc aatggcaaaa accacgcagc
     1501 atacagtaaa tggaaccaga caaccattga caacctgaag aacaagtatt cttacaacgt
     1561 ggatatgtca ggagctcagg ttgcaaccat tgaaaatgga aaactgacag gcactggctc
     1621 agacaccacc gatataaaaa ataaggactt aatatttact ggcggtggag atatcctcct
     1681 gaaatcctct tttgataatg gtgctggcgg tcttgtcttt aatgataaaa agacctatcg
     1741 agtaaacggg gatgatttca cctttaaagg tgccggtgtt gatacaagaa acggcagcac
     1801 cgttgagtgg aatatccggt atgataataa agacaacctt cacaaaattg gtgatggcac
     1861 attagatgtc cgaaaaaccc agaacaccaa cctgaaaaca ggtgagggtc ttgtcattct
     1921 tggagctgaa aaaacattca ataatatcta cataaccagt ggtgatggaa ctgtccgact
     1981 gaatgcagaa aatgcactgt ctggcggtga atacaacggt attttctttg cgaaaaatgg
     2041 cggaactctt gacctgaacg gatataatca gtctttcaat aaaattgctg caactgattc
     2101 aggtgctgta ataaccaata cgtcaaccaa aaaatccatt ttatccctga ataatactgc
     2161 tgactatatc tatcacggca acataaacgg gaatctggac gtacttcagc atcatgagac
     2221 gaaaaaagag aaccgtcgtc ttattcttga tgggggcgtg gacacaacaa atgatataag
     2281 cctgcgtaat acacaactgt ccatgcaggg acatgccact gaacatgcca tttatcggga
     2341 tggagctttc tcttgttcac taccagctcc tatgcgcttt ttgtgtggca gtgattatgt
     2401 tgcaggaatg caaaatacag aagctgatgc tgtaaaacaa aacggaaatg cctataaaac
     2461 caacaatgct gtctctgatt tatcgcagcc agactgggaa accggaacat tcagatttgg
     2521 aacgctacat cttgaaaatt ccgatttttc tgttggtcgt aatgcaaatg taatcgggga
     2581 cattcaggcc agtaaatcaa acattactat tggtgacact acagcatata ttgatttgca
     2641 tgctggtaaa aatattaccg gtgatggttt tggcttccgc cagaatattg tgcgtggaaa
     2701 ctcacaagga gaaacgctgt ttacaggagg gatcacagca gaagacagca ctatcgttat
     2761 taaagataaa gcaaaagcat tattttcaaa ttatgtatac ctgctgaaca caaaagcaac
     2821 catagagaac ggtgctgatg tgacaactca aagtggtatg ttctccacga gcgatatcag
     2881 catctctggt aatctgtcca tgacaggcaa tcccgacaaa gacaataaat tcgagccctc
     2941 aatatatctg aatgatgctt cttatctact gactgacgac tccgccagac tcgttgccaa
     3001 aaataaagca tctgtggtgg gagatataca ctccactaaa agtgcatcca tcatgtttgg
     3061 tcatgatgaa agcgacctct cgcagttgtc tgacagaacc tcaaaagggc ttgcacttgg
     3121 tcttttaggt ggctttgatg tctcatatcg cggttcagtc aatgccccgt cagcatctgc
     3181 cactatgaac aatacctggt ggcaactaac cggagattct gcgctgaaaa cactgaaaag
     3241 tacaaacagc atggtctatt tcactgacag cgcaaacaat aagaaattcc atacgctgac
     3301 ggtcgatgag ctggcaacca gcaacagcgc ctatgcgatg cgtacaaacc tttctgaatc
     3361 agacaaactg gaggtcaaaa aacacctgtc tggtgagaac aatattttac tcgttgattt
     3421 ccttcagaaa ccaacgcctg aaaaacaact gaatattgaa ctggtaagcg cgccaaaaga
     3481 caccaatgaa aatgtcttta aagccagcaa acaaaccatt ggtttcagtg atgtaacgcc
     3541 ggtcattaca accagggaaa ccgatgacaa aataacatgg tcactgacag gctataacac
     3601 ggtagcaaac aaggaagcaa cccggaatgc cgccgccctg ttctctgttg actataaagc
     3661 gtttctgaac gaggtcaaca acctgaacaa acgtatgggt gacctgcgtg atatcaacgg
     3721 cgaagccggt gcatgggcac gcatcatgag cggtaccggc tctgccagtg gtggtttcag
     3781 tgacaactac acgcacgttc aggtcggggt cgacaaaaaa cacgagctgg acggactgga
     3841 tttgtttacc ggtttcactg tcacacacac tgacagcagt gcctccgccg atgttttcag
     3901 tggtaaaacg aagtctgtgg gggctggcct gtatgcttcc gccatgtttg attccggtgc
     3961 ctatatcgac ctgattggca agtatgttca ccatgataat gagtacactg caacctttgc
     4021 cggactcgga acccgtgatt acagcacgca ttcatggtat gccggtgcag aagcgggcta
     4081 ccgctatcat gtcactgagg atgcctggat tgagccacag gctgagctgg tttacggttc
     4141 tgtatccggt aaacagtttg catggaagga ccagggaatg catctgtcca tgaaggacaa
     4201 ggactacaat ccgctgattg gccgaacggg tgtggatgtg ggtaaatcct tctctggtaa
     4261 ggactggaaa gtgatagccc gtgccggtct gggctaccag ttcgacctgc tggctaacgg
     4321 cgaaaccgta ttgcgggatg catctggtga aaaacgcatc aaaggtgaaa aggacagccg
     4381 tatgctgatg tccgttggcc tgaatgcaga aatcagggat aacgtccgct ttggactgga
     4441 gtttgagaaa tccgcctttg gtaagtacaa cgttgataat gcagtcaacg ctaatttccg
     4501 ttactcgttc tgatactccg tattcaaaag ggcctgcggg ccctttacgc tcagtgcctg
     4561 ccgccctgat atgtctgaga gacatttccg ggatgtcagt cgactggttt acttaactgc
     4621 gatgactaaa gctggcagta atgccggctt ttttacggcc acatacgtct tcctctcatc
     4681 cggaactgtg atacaaatcc tgcatacagg attgaccgcc ggttcctgca aatggcacat
     4741 tttcagtaag atagcgaagg ttacattccc ccgtcatgtg tggcacaagc tgtcaccggg
     4801 aaagaaacgg aaagcacatg aaaaacatca aaactcacac cggactcctt atccacaaag
     4861 aacaaacgcg gcgcgttcgt cttcatgaaa cacccactgc ctggtgccac acgcaccggg
     4921 aatgctacag caaaacgacc ggtcgccgct gcggcagccc tgacagtctc agcaggctga
     4981 ttctgagca
//
LOCUS       DAZFWL020000233         4931 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFWL020000233 DAZFWL020000000
VERSION     DAZFWL020000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.234"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(116..874)
                     /gene="chbG"
                     /locus_tag="ACHKNP_RS24165"
                     /old_locus_tag="pgaptmp_004833"
     CDS             complement(116..874)
                     /gene="chbG"
                     /locus_tag="ACHKNP_RS24165"
                     /old_locus_tag="pgaptmp_004833"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HGU4706835.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(887..2239)
                     /gene="celF"
                     /locus_tag="ACHKNP_RS24170"
                     /old_locus_tag="pgaptmp_004834"
                     /gene_synonym="chbF"
     CDS             complement(887..2239)
                     /gene="celF"
                     /locus_tag="ACHKNP_RS24170"
                     /old_locus_tag="pgaptmp_004834"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGU4706836.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(2344..3186)
                     /gene="chbR"
                     /locus_tag="ACHKNP_RS24175"
                     /old_locus_tag="pgaptmp_004835"
     CDS             complement(2344..3186)
                     /gene="chbR"
                     /locus_tag="ACHKNP_RS24175"
                     /old_locus_tag="pgaptmp_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HGU4706837.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(3194..3544)
                     /gene="chbA"
                     /locus_tag="ACHKNP_RS24180"
                     /old_locus_tag="pgaptmp_004836"
     CDS             complement(3194..3544)
                     /gene="chbA"
                     /locus_tag="ACHKNP_RS24180"
                     /old_locus_tag="pgaptmp_004836"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HGU4706838.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEVKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(3595..4931)
                     /gene="chbC"
                     /locus_tag="ACHKNP_RS24185"
                     /old_locus_tag="pgaptmp_004837"
                     /pseudo
     CDS             complement(3595..4931)
                     /gene="chbC"
                     /locus_tag="ACHKNP_RS24185"
                     /old_locus_tag="pgaptmp_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
BASE COUNT         1341 a         1302 c         1052 g         1236 t
ORIGIN      
        1 agcgcatcag gcagttttgc gtttgtcagc agtctcaagc ggcgcagtta cgccgccttt
       61 gtaggaatta atcgccggat gcaaggttca cgccgcatct ggcaaacatc ctcacttaca
      121 catcccgata actccccaac cgataaccac gctgagcgat agcacctttc aacgacgctg
      181 atgtcaacac atccagctcc gttaagcgtg ggaaacagta agcactctga cggatagtat
      241 tatcgataaa cgccggatga cacatcacct ccagcgaacg atcaccccga tggcttgcgt
      301 catccagcac ttgcaggaac agtgcctcgc taatctcttc accataaaac gcgctactga
      361 atccctgaga actgcgcaga ttagccggta aatcaccagc gttagatagc ggctgacggt
      421 cgatgcgcaa cgcaatcccc tcttcagccg caaacctggc aacaatcggg aaaatctgcg
      481 ggaacatatg cacatgatga tggctatcaa gatgcgtagg tttgcgtcca aatagctcaa
      541 tgaaacgcaa atactgactg gcaagctcct gagtaatttc ttccagcggt aaagcatctt
      601 cttctgccaa ctgccagatc cattttccca gcacaccatc gcgggttaac cccggcatag
      661 ctgtcagggg cttgcccata gtaaggacaa agtgcatccc tatggccaga ctcggttcat
      721 cacgactcaa ctgcaccgca tggtcaatag cctgcccatt caccagcgcc gtcgttgatg
      781 tgacaatccc attgcgacag gcctcgataa tgccgtagtt ctgacctttg cttaagccaa
      841 aatcatcggc attaacaatc agtaagcgtt ccataatcag cctcggttaa tgtgcttttt
      901 taagctctgc gatgcagtcg gcaaaatttg gcagccattt ctcgtgcgcc agaatcatct
      961 cgcgtgccag cagctcagca tcgcgatcgg aatgcaccaa cggactaagg tttagcgcca
     1021 gtaacacatc gttaaattct ccgctaagtg cggcgttgct ggcagcaatc tcgaagcctt
     1081 taatggtgtg aatcagcccc atcactttat catcgaaatg cgtaatgcgc ggatgtggcg
     1141 tcgcgccatc gcgccccagc ttacaggtca tttctaccgc ccagtctgcc ggaatattat
     1201 caatatgccc atgatgcggg atattaacgt aatgttcagc ttgcttgtcg ttgtagatag
     1261 cgttgatcac ttcgcacgct gcatcagagt aataagcccc accgcgctgt tccagttctt
     1321 tcggcttaac tttcaactcc ggatttttat acagctcaaa aagttgtttc tcgactttct
     1381 gtactacctg tgctcgtgcg ccgcctttgt agtattcgcc catttcaata gccagcatct
     1441 ctttctgctt gaagtaatac agcagataag aacatggcag cagatttaac gaacgaatta
     1501 agccctcact aaatggcaga tcgaaaatat tttttacaga ggacgctttt aactgccctg
     1561 acgccacacc atcaagcaat tcggcaaagc gcgacttgcc atttaccagc acatccttaa
     1621 tgaacaccat atggttgagg ccgaacagat cgatagataa atcatcgctg tctttcagca
     1681 tcagaacatc gcgaataaac atcttcatgc cgatcggaat attacacacg ccgataaagc
     1741 gtttaaatcc ggtatgacga taaacggctt cagtgaccat tcccgccggg ttagtgaagt
     1801 taatcaccca tgcattcgga caaagttctt cgacatcttt tacgatgtca aaaatcaccg
     1861 gaatggtacg cagaccttta aacagaccgc ccgcgccgtt ggtttcctga ccaagataac
     1921 catgacttaa tggaatacgt tcatccagtt cgcgcgccgg taattggcct acgcgcagtt
     1981 gggtagtaac gaaatcagca tctttcaatg cttcgcggcg atccagcgtt ttataaagct
     2041 tcatcgggac gccagcgtta tcaatcatcc gttggcagag ttcaaaaatg atatccagtt
     2101 tctctttacc atcttcgaca tccaccagcc ataattcgct gaccggcaat tcgtgataac
     2161 gcttaataaa tccttccagt aactccgggg tatagctgct cccgccacca atagtgacga
     2221 cttttaattt ctggctcata atttctccct tcagtacaga atactgatat ctggcatatc
     2281 tgcccccccg gacataaata atccagcaac aggacagata tgtgaattgt caggtataac
     2341 gacttactga ttaaattcag tcaatttctt acgatagctc ttaggcgtaa aggacgttaa
     2401 tttcttaaac gttttaataa acaaactcgg gctactataa ccggcctcaa acgcaatatc
     2461 cgtcacggaa tagttggtca tttccagttg ttttttggca aaattaatac ggatctcatt
     2521 aataatctgc attggtgttt tgccataata tcgttgagtg gctcgcgtca aatattcctg
     2581 tgatttggct gacaacgtca ccatattctc cagcgccgat tcactaaact gctctttatc
     2641 atgcatcttt tctaccgtac ttttcagcca ctgcggtaca tcatcaatca cctgttcttc
     2701 gcggtaatga cgtaaacggt taatgacata aaaagtaacc atctcaacaa actcttccag
     2761 ccctgtttcg cggaaattca atgaagaaat cactgtttcc acataggtga gaaaagcgtt
     2821 attggtccgg tatacctgcg aagcgacaaa gcaataaggc aacaacggca ggtaatgctg
     2881 ctcaaaaaag cgtttactga tcccaacgtt caatatacgc gtggcaccaa actcataaaa
     2941 actttgatgg tgcgaaccta acggaataaa aacaaaatcg ccccgttcca gtaacacgcg
     3001 cttaccgtta atctcctgga aataacgccc ggttaatacc agagtaaatt cataatagtc
     3061 gtgctgatgc agcccgctga tactctcagt tttgttatag ataaacacat ggaagttttt
     3121 gccattaaac aactgctgtt ctcgggcagt ggcaatttcc ggcgcgttaa tcactggctg
     3181 catcatcgac tccttatgcc ttcagttttt catgaagctc aattaattca gtaatcagtt
     3241 cacgcgcaag catggacgtc attaaatgat cctgagcgtg gaccagcacc agactcactt
     3301 tcatcttaac ttcgcccgca tcgccttcaa tcagtttcgt ctgtaccaga tgcgcttcat
     3361 tcaatgccat ccgtgactga tccatcatgg cttttgctgc agcaaaatcg ccctgcttcg
     3421 cctgtttcag tgccgcatac gccaggctgc gcgcttgtcc ggagttgatg ataagcccca
     3481 tcaccacttc ttccagctct tcagcttctg tttgcgtatc aggaatatta tcgagatcca
     3541 tcatacatcg ttcctctctc ttcttaccgg caagattacc cgtaccggca tcgattaaaa
     3601 tttcagggcg ttagcgatat cttcttcgct ctcttcttta tcaatcgcat tttgtgcttt
     3661 gttagccacc acaacaaagg gcagataaat taaggttgcg atgccaaggt tgaagagtgc
     3721 gaccagcaat gcggcgacgc taccgttggt gttaaagaag gctcccagac cggttggcat
     3781 ggtccacggt gcaatattgg tcaccggagg aatgatgccc atgtagtacg ctgcgagggt
     3841 gattgccgcc agaatcggtt gtaccagtac aaacgggata aacatcaccg ggttcatgat
     3901 aattggcaga ccaaacagaa tcggttcgtt aatctggaag atgccggacg gcagcgccag
     3961 ttttgccacc tgacgataat cagcacggcg agaggcgata aagatagcca ggatcaggcc
     4021 taaagtcgca ccactgcccc caaggaaaat aaaggagtcc agcatcggct tggcccagat
     4081 atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa ccatattgct gataggtcgc
     4141 gatattttcc agcgcccacg gcgtcataat gccgttgtcc agtgcggtca gcgccagcgc
     4201 gccatgaata ccgaagaacc agagcagtgg aacaaagatc acataggccc agcccaccac
     4261 gctacccaac gatgccagtg gggttgagat ggtatccata atgatctgat ggaagttggt
     4321 gccccaggta ttcaacgccc aggcaataat ccccatcacg gaaagaataa taaagccggg
     4381 aattaatgcc gagaaggaac gcgatactga agcaggtacg ctgtcgggca gtttaatgac
     4441 ccaattgcgg cggacaataa aggtaaacat ttctgccacc accaggccaa taataatccc
     4501 ggagatgata ttcgccccac ctaaccagtt tgcgccaacc gcataggcct caccgacact
     4561 atatgggggt gacggtcata aatgctgcaa cggataacaa ccccgccgcc agcgcatcga
     4621 ctttacgctc ttctgccagc gccatgccaa taaagaacgg tgccattaaa gacattatcc
     4681 ctaatgttcc gttatatacg ttgccgccaa tacctttcag gccattaagt gtttcaatgg
     4741 ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga cccctcccca aagcttagaa
     4801 aaacgttgtt aattaataca aacatggccc ccgcaagggt taacggcatt aagcgaataa
     4861 agccattttt gattgcatta acgtgtggct gctttcctat tttaactgca aaagggagga
     4921 gtaccttttc a
//
LOCUS       DAZFWL020000234         4861 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFWL020000234 DAZFWL020000000
VERSION     DAZFWL020000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.235"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..456)
                     /locus_tag="ACHKNP_RS24190"
                     /old_locus_tag="pgaptmp_004838"
     CDS             complement(<1..456)
                     /locus_tag="ACHKNP_RS24190"
                     /old_locus_tag="pgaptmp_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HGU4706839.1"
                     /translation="MTISYIPILKAKRSELSALSQLSIEKKSKILPLLEIEPVPIDPD
                     SGIALKSYNETLIEFGKKVSKSCSDMQGVYIDGLLIEEHFISPEDHYPIINAVNQVRD
                     MEIRVIPVSSPTRPSNYKRAIDELMQNEICLRLTTLDLVNPQLITHYINH"
     gene            1014..1169
                     /gene="hokD"
                     /locus_tag="ACHKNP_RS24195"
                     /old_locus_tag="pgaptmp_004839"
     CDS             1014..1169
                     /gene="hokD"
                     /locus_tag="ACHKNP_RS24195"
                     /old_locus_tag="pgaptmp_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU4706840.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1211..1390)
                     /locus_tag="ACHKNP_RS24200"
                     /old_locus_tag="pgaptmp_004840"
     CDS             complement(1211..1390)
                     /locus_tag="ACHKNP_RS24200"
                     /old_locus_tag="pgaptmp_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706841.1"
                     /translation="MPDGKVGIRIVKFGFVHHLFSLLAQQVSGVQADVRIVNQNARKK
                     QNPPKRVKCGCVEDA"
     gene            1337..1609
                     /locus_tag="ACHKNP_RS24205"
                     /old_locus_tag="pgaptmp_004841"
     CDS             1337..1609
                     /locus_tag="ACHKNP_RS24205"
                     /old_locus_tag="pgaptmp_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706842.1"
                     /translation="MVHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            1611..2666
                     /locus_tag="ACHKNP_RS24210"
                     /old_locus_tag="pgaptmp_004842"
     CDS             1611..2666
                     /locus_tag="ACHKNP_RS24210"
                     /old_locus_tag="pgaptmp_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4706843.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRNQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWATINNVADALPETVLRRSLGLRAEKIRSVYRESDIVPGEQTATSMLKQR
                     TKNIALLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPRREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            2667..3032
                     /locus_tag="ACHKNP_RS24215"
                     /old_locus_tag="pgaptmp_004843"
     CDS             2667..3032
                     /locus_tag="ACHKNP_RS24215"
                     /old_locus_tag="pgaptmp_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4706844.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQRLKLNLSGRLVIKIIAEPPDKRRRDLDNILKAPLDVLTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELECA"
     gene            3029..3718
                     /locus_tag="ACHKNP_RS24220"
                     /old_locus_tag="pgaptmp_004844"
     CDS             3029..3718
                     /locus_tag="ACHKNP_RS24220"
                     /old_locus_tag="pgaptmp_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU4706845.1"
                     /translation="MNNHYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            3914..3989
                     /locus_tag="ACHKNP_RS24225"
                     /old_locus_tag="pgaptmp_004845"
     tRNA            3914..3989
                     /locus_tag="ACHKNP_RS24225"
                     /old_locus_tag="pgaptmp_004845"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3947..3949,aa:Ile,seq:cat)
     gene            4080..4153
                     /locus_tag="ACHKNP_RS24230"
                     /old_locus_tag="pgaptmp_004846"
     tRNA            4080..4153
                     /locus_tag="ACHKNP_RS24230"
                     /old_locus_tag="pgaptmp_004846"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4114..4116,aa:Arg,seq:tct)
     gene            4330..4758
                     /locus_tag="ACHKNP_RS24235"
                     /old_locus_tag="pgaptmp_004847"
                     /pseudo
     CDS             4330..4758
                     /locus_tag="ACHKNP_RS24235"
                     /old_locus_tag="pgaptmp_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310776.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
     gene            4755..>4861
                     /locus_tag="ACHKNP_RS24240"
                     /old_locus_tag="pgaptmp_004848"
     CDS             4755..>4861
                     /locus_tag="ACHKNP_RS24240"
                     /old_locus_tag="pgaptmp_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU4706846.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLV"
BASE COUNT         1294 a         1085 c         1263 g         1219 t
ORIGIN      
        1 atgattaatg taatgcgtta ttaattgtgg gttaaccaga tctaacgtgg ttaatctcaa
       61 gcatatttca ttctgcatta attcatcaat cgctctttta tagttagatg ggcgagttgg
      121 tgaactgaca ggaataactc ttatctccat atctctaact tgattaaccg catttattat
      181 agggtaatga tcttcaggag aaataaaatg ctcttcaatt aataatccat caatataaac
      241 accttgcata tctgagcaag attttgagac tttctttccg aactctataa gagtctcgtt
      301 ataactcttt aaggcaatac ctgaatcagg gtcaattggc actggttcaa tttcgagtaa
      361 tggcaaaatt tttgatttct tttcaatgga tagctgcgat aaagctgata actcagaacg
      421 tttcgctttc agaataggaa tatatgaaat tgtcatgtta ataccttata cagaatcaat
      481 taacaattaa tggttcttcg gacattgaaa tttatatcgc aaaaattggc aaactctcct
      541 cgactactca cttgtgggta tcctggcgta aaaggaatga cttggcaata cccggtgtga
      601 tcataagtca ttgaaaatga tcatttttat cagtctttct tccatgatga atgctaatgc
      661 catttgattt gttgaggtga aaactgttaa aaatcaaaac gatgtaattg aaatgaacgt
      721 tcggtagcat tcacgcttta aatgtttctt ttgtgctgat tggatgaatt tgggtcactt
      781 ctgatgagag atgttgcagg aaaagaagtt ggcattgatc tattggatag ttagaattgc
      841 tgcgggtgct tgaggctatc tgcctcaggc atgaacacca aaaggcagat agagaaaagc
      901 cccagttaac attacgcgtc cggcaagacg cttaacatta atctgaggcc aatttcatgc
      961 tttgcacatg taggttagcc tcttacgtgc cgaaaggcaa ggagaagcag gctatgaagc
     1021 agcaaaaggc gatgttaatc gccctgatcg tcatctgttt aaccgtcata gtgacggcac
     1081 tggtaacgag gaaagacctc tgcgaggtac gaatccgaac cggccagacg gaggtcgctg
     1141 tcttcacagc ttacgaacct gaggagtaag agaccaggcg agggagaaat ccctcgccac
     1201 ctctgatgag tcaggcatcc tcaacgcacc cgcacttaac ccgcttcggc gggttttgtt
     1261 ttttcctggc attctggttt acaattcgca cgtcagcctg aacacctgac acctgctgcg
     1321 ccagcagaga aaacagatgg tgcacaaaac caaatttcac aattctgata ccgaccttgc
     1381 catccggcat gggcggcgtt cacacgcatt taaaaccgac tggtaccaac acccaccatg
     1441 tactgaagaa caggccgaat ggctaattca taactaccgc agacgcggat acgagattaa
     1501 gaaagccctc agcctcgatt atcgtcactg gataatctat gtcaggctcc cttattccga
     1561 acgcccaccg cgtccgtccc gcacattcca gcaacgcatc tggaggtaac gtgcgggtat
     1621 tacttcgacc tgttccggta ccggaacttg ggctggtggt gctaaaaccg ggccgtgaat
     1681 ccatgcaggt atttcataac cctcgagttc tggtggagcc ggaaccgaaa agcatgcgcg
     1741 gtctgccgtc cggagtcgtt cctgctgttc gccagccgct ggcggaggat aaatcattac
     1801 tgccattttt cagcgatgag cgggtgattc gtgctgctgg tggtgctggt gcattgtctg
     1861 actggctgtt acgccacatt aaatcctgcc agtggcctca tggtgactac catcacagtg
     1921 aaaccgtcat acatcgttac ggtaccggcg cgatggtgtt gtgctggcac tgcgacaacc
     1981 agctgcgtaa ccagacttcc gaatcactcg ggcagcttgc tcaccaaaac ctgtcagcat
     2041 ggatgattga tgtcatccgc cacgcaatga atggcacgca ggagcgggaa ttatcgctgg
     2101 ctgaattatc ctggtgggca accataaata acgtagcgga cgcactaccg gagacggtgt
     2161 tacgtcgttc actgggatta cgcgcggaaa aaattcgctc agtataccgc gagagcgaca
     2221 tcgtgccggg agagcagacc gccaccagca tgctgaagca gcgcacaaaa aatattgcgc
     2281 tactgcctca cgcccaccag caaaacccgc cacaggaaaa gacggtggta agcattgccg
     2341 ttgatccgga gtcaccggct cagtatctcc agcgccagaa accacgacgg gaagagatgc
     2401 ctgtatacac gcgttgggta aaaacgcaga aatgcatgac gtgtggtaat caggcagatg
     2461 atccgcatca catcattggt catggactgg gagggatggg aacaaaggct gatgatttgt
     2521 ttgttattcc gctgtgccgt aaatgccata gcgaactaca cgccggggta aaagattttg
     2581 aagaaaaaca cggcagccag ctgttgttgc tgattcgttt tttaatgcac gcgagaaatt
     2641 cgggtgtcct gaagtggaaa gcatgaatga ctgaacggat agaatttgtt ttgccttacc
     2701 cgccgacggt gaatacctac tggcgacgtc atggcaatac gtatttcatc tcggaagccg
     2761 gaaagcgtta tcgccgtgat gtggcgctaa ttgttcgcca gcagcggctg aaattaaacc
     2821 tgtccggaag gctggtgata aagattattg cagagccacc ggataagcgc cgtcgtgacc
     2881 tggacaatat cctgaaagca ccactggatg tgctgacgca tgccggactt ctcatagacg
     2941 acgagcagtt tgatgaaatc aatattgtgc gcggtcagct cgttcctggt gggcggctgg
     3001 ggataaaaat cacagaactg gagtgcgcat gaataaccac tatttacagt ttgtgcgtga
     3061 gcagctcatt atcgccaccg ctgatttgag tggggcaaca aaaggtcagc ttgaagcctg
     3121 gcaggagaat gccatgttcg atacagggcg ttacaggcga aaaaaaatcc ggtaccgcga
     3181 tgaagtgact ggaaaaatga taacgcggga taatccacca atcccgggaa agcaatcgct
     3241 ggcgaaggtg acgtcaattc ctctggtcag tccggttgag ttttcgacat catcgtggcg
     3301 gcgggctgtt ctgtctcttg aagaacatca taaagcctgg ttgttgtggt gttacagcgg
     3361 gagtatttgt tgggaatatc agatcgcgat aacacagtgg gcgtggaatg aatttaatac
     3421 tcaatccggt accagaaaaa ttgctgggaa aacgcaggaa cgcctgaaaa aattaatctg
     3481 gctggcggcg caggcagtaa aagcagaact ttttggtggg gaaggttatg aatatcagga
     3541 gctggcatta ctggcgggag tgacaactaa aaactggtcc aaaacattta ctcgtcactg
     3601 ggttgcaatg aaacacattt ttcaccgact ggatagtgag gctttattat ttgtaatgag
     3661 aacacgttca aaacaaaagg cggcattttc aaagcaaagt gttgcaaaag tagattgaaa
     3721 ggcatatatt tcatgcaaat ctgatatttt gccgattttg tacgtgatgg caaaagcaaa
     3781 caaaacccgc ccacaagcgg gttttgttgt gccacttatc tcggatagac atggtgaatg
     3841 cgctggtgga ggagataagg gtgatttttg tatgcttgca acattgattt cgtaacgtta
     3901 ttatcctgcg cccggccctt tagctcagtg gtgagagcga gcgactcata atcgccaggt
     3961 cgctggttca aatccagcaa gggccaccaa ccgccactag ccatcaggaa agagcgtcaa
     4021 ccctttaagt tgagtgtgcg aggttcgagt ccccggtggt ggtccattat cggtattctg
     4081 cgttgttagc tcagcaggac agagcaattg ccttctaagc aatcggtcac tggttcgact
     4141 ccagtacaac gcgctacact tattttcccg gctcgctttt gcgggccttt tttgtatctg
     4201 cgccacgccc ggcgcatatc aaccacagag cctttcgggg gtgagcttac ggagtggtca
     4261 gtgtgacttt ctctgtgggc agatcgctcc cgggcgttgg ctcacccacc caaaggaacg
     4321 tcacgatgtt tggtattttt ggtaaaaaaa gcccgcagag cggcaacgga aattaaaaag
     4381 tttgaaaaac gcgatctggc acaggtggtg attaacgccg cctacctggt ggcctatgca
     4441 gatggtgaat gtgaggcttc ctagaaagcg aagatcgaac aggtactgcg taatcagcct
     4501 gcgctgtacg cgtttacgtc agaaattaat gcgattagcg caaccattat cggtcagctg
     4561 gatacgaact ttaaaactgg tcgtcgtgcg gcgttacgtg agatcgagga tgtgaaacac
     4621 gatacgcgtg aagcggaaga tgtgctggat gtggcggtgg ccattgcgga ggcagacggc
     4681 gaaattgagc cggaagagcg caaggtgctg gaagagattg ccggtgttct gggtcttcgt
     4741 ctggagaatt acctgtgacg gtaaaactgc gcctgactgt ggctgcactc ctgctgtttc
     4801 tggtggtgat ggtggatttc accagcagaa tcatgtcggt gctggcggat ggggtgctgg
     4861 t
//
LOCUS       DAZFWL020000235         4841 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFWL020000235 DAZFWL020000000
VERSION     DAZFWL020000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.236"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            155..1129
                     /gene="hemB"
                     /locus_tag="ACHKNP_RS24245"
                     /old_locus_tag="pgaptmp_004849"
     CDS             155..1129
                     /gene="hemB"
                     /locus_tag="ACHKNP_RS24245"
                     /old_locus_tag="pgaptmp_004849"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HGU4706847.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1235..2086)
                     /gene="tauD"
                     /locus_tag="ACHKNP_RS24250"
                     /old_locus_tag="pgaptmp_004850"
     CDS             complement(1235..2086)
                     /gene="tauD"
                     /locus_tag="ACHKNP_RS24250"
                     /old_locus_tag="pgaptmp_004850"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HGU4706848.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTIAPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2083..2910)
                     /gene="tauC"
                     /locus_tag="ACHKNP_RS24255"
                     /old_locus_tag="pgaptmp_004851"
     CDS             complement(2083..2910)
                     /gene="tauC"
                     /locus_tag="ACHKNP_RS24255"
                     /old_locus_tag="pgaptmp_004851"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HGU4706849.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2907..3674)
                     /gene="tauB"
                     /locus_tag="ACHKNP_RS24260"
                     /old_locus_tag="pgaptmp_004852"
     CDS             complement(2907..3674)
                     /gene="tauB"
                     /locus_tag="ACHKNP_RS24260"
                     /old_locus_tag="pgaptmp_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HGU4706850.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3687..4649)
                     /gene="tauA"
                     /locus_tag="ACHKNP_RS24265"
                     /old_locus_tag="pgaptmp_004853"
     CDS             complement(3687..4649)
                     /gene="tauA"
                     /locus_tag="ACHKNP_RS24265"
                     /old_locus_tag="pgaptmp_004853"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4706851.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1112 a         1401 c         1242 g         1086 t
ORIGIN      
        1 tcagtgaata tgaaattatt gctatttcta ttacaaagat gtcctggtct ctctttcatt
       61 ctcttcttgt caacatcacg acaactttcg taaaacactc ctgccccgct tcaggtatac
      121 tgtgcacctt aattccacaa acatcaggca gaccatgaca gacttaatcc aacgccctcg
      181 tcgcctgcgc aaatctcctg cgctgcgcgc tatgtttgaa gagacaacac ttagccttaa
      241 cgacctggtg ttgccgatct ttgttgaaga agaaattgac gactacaaag ccgttgaagc
      301 catgccaggc gtgatgcgca ttccagagaa acatctggca cgcgaaattg aacgcatcgc
      361 caacgccggt atccgttccg taatgacttt tggcatctct caccataccg atgaaaccgg
      421 cagcgacgcc tggcgggaag atggactggt ggcgcgtatg tcgcgcatct gcaagcagac
      481 tgtgccagaa atgatcgtca tgtcagacac ttgtttctgt gaatacactt ctcacggtca
      541 ctgcggtgtg ctgtgcgagc atggcgtcga caacgacgca actctggaaa atttaggcaa
      601 gcaagccgtg gttgcagctg ctgcaggcgc agacttcatc gccccttccg ccgcgatgga
      661 tggccaggta caggctattc gccaggcgct ggacgctgca ggctttaaag atacggcgat
      721 tatgtcgtat tcgaccaagt tcgcctcttc cttttatggt ccgttccgtg aagctgccgg
      781 aagcgcatta aaaggcgacc gcaaaagcta tcagatgaac ccaatgaacc gtcgcgaggc
      841 gattcgtgaa tcactgctgg atgaagccca gggcgcagac tgcctgatgg ttaaacctgc
      901 tggagcatac ctcgacatcg tgcgtgagct gcgtgaacgt actgaattgc cgattggcgc
      961 gtatcaggtg agcggtgagt acgcgatgat taagttcgcc gcgctggcgg gtgctataga
     1021 tgaagagaaa gtcgtgctcg aaagcttagg ttcgattaag cgtgcgggtg cggatctgat
     1081 tttcagctac tttgcgctgg atttggctga gaagaagatt ctgcgttaat ttttttcctc
     1141 tcagaaaggg ggagttaacc tgttgaaagt acggtgaacg ccccctcacc ctaaccctct
     1201 ccccaagggg gcgagggaac cgtccgcact cgtatcaccc cgcccgataa aacggtttat
     1261 ccccaaggat cgtcgcccga tgcataatcc gccgctgtgg caggtaatcg gcattggcat
     1321 agtgctgagt cacgcggtta tcccaaatcg caatatcatt cggttgccag cgccaacgca
     1381 cctgaaactc cggtttagtg atatgggcga ataaaaagcc taacaaggct tcgctctctt
     1441 tctcgctgac atcgacaatt cgcgtagtaa agccttcatt cacaaacagc gcctgtttac
     1501 cgctcaccgg atgcgttcgc accaccggat gtagcaacgg aggatttttc gcgaccgcct
     1561 cgcgccagcg ttgatgttcc tcctcggttt tgcggtattt gtattccggg aacgatttac
     1621 ggaaatcatg ctccgcacgc agcccgctca gcagctggcg gaagggaaca gagagcgcct
     1681 cataggccgc aataccgctg gtccagagcg tatcaccgcc ggtcgaaggt aactctttag
     1741 ctgccagaat cgcccctgcg ggtggcgttt caataaatgt cacatcggta tgccagttgt
     1801 cgttatccgg cggattatcg ttatgggtat ccagcacgat gatctcgtca accccttcgg
     1861 catgagggta aacagggtga atatgcagtt cgccaaaacg ctgggccagc gcgcgttgct
     1921 gctgcggcgc aatagtttga tcgcgcagaa acaccacctg atggcgcagc accgcatggt
     1981 aaagctgttc aaactgatta tcgcttaacg ggcgcgtcag gtcggcaccc gaaatttgtg
     2041 cgccgatata cggccccagc ggggtaatgc tcagacgttc actcattgta cttctccatg
     2101 ccagggcgtc agacggcgct gtaacgcgcg caaacccagt tctaaaagaa aggcgataat
     2161 cgcaatcacc gcgatccccg ccagcaccac gtcagtcgcg agaaattcac ccgctgactg
     2221 aaccataaat cctaaaccgc gcgtcgcggc aatcagctcc gccgctacca gcgtagacca
     2281 gcccacaccc agaccaatgc gtaatccggt gaggatttcc ggcagcgcgc cgggcaaaat
     2341 gacaaaccac agcacctgcg cacggctggc acccagcgac tgggcggcac gaatgcgaac
     2401 ctgctgcgcg cttttcaccc ccgccagcgc cgacatcgcc accggtgcaa aaatcgctaa
     2461 atagatcagt aagatcttcg aggtttcacc aataccaaac cagatcacca tcagcggcaa
     2521 ataagccagc ggcggcaccg gacgataaag ttcgattacc ggatccagaa tgccgcgcac
     2581 cgtcgggctt agccccatgg cgatcccgac cggaataccg atcaccaccg ccgcgagtag
     2641 cgccagcaca atgcgcgtca ggctggctgc cagatgctgc cacagcgtgg cgtccataaa
     2701 tccctgcggt ccggcaatgg tgagcagttt tgccagtact tgttgcggcg gcggcaaaaa
     2761 cagcgggcta atcagttgca gcgccgccac cgtccaccat acagtgagta aaaccgctaa
     2821 cgtgccaatg cttaaggtca cctgacgcga gagcggccag cgccatttca gccgccgcga
     2881 atgcagtttt tcattaatga gcacactcat gagaacgcct cccgttgctc aaatacgcgg
     2941 cttaaaacat attcgcgcat ggcgatgaat tgtggatcgg acttgatgct gcggctcgac
     3001 tctcccgcaa caaagcggcg agcaaagttg agcggcagcc gctccagcac gcggccaggg
     3061 ccgggtgaaa gcagaaccag ttcagtcgcc ataaacaccg cttcttctat atcgtgggta
     3121 atcaacagca cctgcttgcc cgtctcctgc cagagtttca gcagcagggt ttgcatctgg
     3181 tcgcgggtga aggcgtccag cgcaccaaac ggctcgtcga gtaataacag ctggggattc
     3241 gccgccagcg cgcgggcaat ccccacccgc tgacgttgac caccggaaag ctgccagata
     3301 tagcgttttt ctgcgccttc cagccccact tttttcagca tctggtgcgc gatttccagt
     3361 cgctgcattt tctctatacc tgccaattgc aggccgaacg ccacgttgtc ctgtacattg
     3421 cgccacggta gcagcccttc attctgaaaa accaccccgc gctctgctcc cggtccctca
     3481 atacgcttac ccgccagttg aatgctgcca tgctgataag gcacaaaacc ggcaatcaga
     3541 ttcagcaggg tggttttacc gcagccggac ggccccagca ccaccagtag ctcgccgctt
     3601 tccagcgtca ggttgatatc ttccagtgcc ggtttgccgc cataatcggc gtaaagatga
     3661 gagatttgca gcatctgcgc ctccttttat tgcacgaagc gcgaggtaac gtactggctg
     3721 taatcattcg ctacagccgg gaccttgccc tgctctttca aaaactgcgc ggtgtcgatg
     3781 atcgctttgt tcaccggtcc ggtcagttct gccgtttgtt gctgcggcgt cagataggta
     3841 ttccccttca ccagccccgg aacgtcacct tccggcacgc cgcttaaacg cgccagtttg
     3901 ctgatgtttt ccggctgttt cagccacgcg tctgggttag caatgtacgg ttgctgagca
     3961 tcgatggcgc ttttagcgaa cgctttcacg acctcaggat gtttctcggc aaaatctttg
     4021 cgcaccaccc agacgtccag cgttggcgca ccccactgcc cgacctgttc agaatcggtc
     4081 agcaccttgc cgtctttttc cagggcgtta accgccggtg cccagacata agcaccatca
     4141 atatctcccc gctgccaggc agcgataatc gcgggcggct gcaggttcac aatctccact
     4201 tgcccgggtt taatgcccca gtgtttcagc gccgccagca ggctgtagtg ggtggtagag
     4261 ataaacggta cggcgatgcg tttgccaatc agatcttccg gtttgctgat agttttcttt
     4321 accaccagcg cttcggagtt acccagtttt gacgccagca agaaaacttc aatcggcacc
     4381 tgttggctgg ctgcaaccgc taacgggctg gaaccgaggt tgccgatttg cacatcgcca
     4441 gaagccagcg cccgcacgat gctggctccg ctgtcaaact tacgccagtc cacggttgct
     4501 ccgctttctt tagcaaaggt gttgtcggcc tgagccactt tcgccggttc ggctgaggtt
     4561 tgatacgcaa cggtgacgtt caccgcctgt gcctgaaaag cgatgaatgc cagtgcggca
     4621 agaagtgtgt tacgcgatga aattgccatg attgtctgct cccctgtctt gttatgtgag
     4681 cagtattcag gaataaaaac attcattaaa agaattagtc gttatcgcac agatgatttt
     4741 attcttagca aaaaaacggc aaaggaaaga ttaaatatgc aattactccg ttctaacaat
     4801 tgggaaaatt actacacttc attttagtga tgcataaaca a
//
LOCUS       DAZFWL020000236         4820 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFWL020000236 DAZFWL020000000
VERSION     DAZFWL020000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.237"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(55..630)
                     /locus_tag="ACHKNP_RS24270"
                     /old_locus_tag="pgaptmp_004854"
     CDS             complement(55..630)
                     /locus_tag="ACHKNP_RS24270"
                     /old_locus_tag="pgaptmp_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU4706852.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(699..1277)
                     /gene="terD"
                     /locus_tag="ACHKNP_RS24275"
                     /old_locus_tag="pgaptmp_004855"
     CDS             complement(699..1277)
                     /gene="terD"
                     /locus_tag="ACHKNP_RS24275"
                     /old_locus_tag="pgaptmp_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HGU4706853.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(1326..2366)
                     /gene="terC"
                     /locus_tag="ACHKNP_RS24280"
                     /old_locus_tag="pgaptmp_004856"
     CDS             complement(1326..2366)
                     /gene="terC"
                     /locus_tag="ACHKNP_RS24280"
                     /old_locus_tag="pgaptmp_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HGU4706854.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(2389..2844)
                     /locus_tag="ACHKNP_RS24285"
                     /old_locus_tag="pgaptmp_004857"
     CDS             complement(2389..2844)
                     /locus_tag="ACHKNP_RS24285"
                     /old_locus_tag="pgaptmp_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU4706855.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(2867..4024)
                     /locus_tag="ACHKNP_RS24290"
                     /old_locus_tag="pgaptmp_004858"
     CDS             complement(2867..4024)
                     /locus_tag="ACHKNP_RS24290"
                     /old_locus_tag="pgaptmp_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU4706856.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(4024..4605)
                     /locus_tag="ACHKNP_RS24295"
                     /old_locus_tag="pgaptmp_004859"
     CDS             complement(4024..4605)
                     /locus_tag="ACHKNP_RS24295"
                     /old_locus_tag="pgaptmp_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU4706857.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         1223 a         1291 c         1161 g         1145 t
ORIGIN      
        1 cggatcgata cccaaaaaaa accccggcat tgccggggtc agactcagcg tcttttagac
       61 attgacgccg tgctgggaag caagcgctga cagaccaccg gcaaaaccct ggccaacagc
      121 tttaaacttc cactcagtac catggcgata cagttcaccg aagaccattg cggtttcggt
      181 tgaggcgtct tcagacagat cgaaacgggc aatttccgtc ccgttgtcgt tgttgtaaac
      241 gcgcatgaag ctgttgctca ccatgccgaa gttttgttta cgcgcttctg catcatagat
      301 ggtaacggca aataccagtt ttttgatgtc tgctgagact ttggtcagat cgattttgac
      361 ctgctcatcg tcgccgtcgc cttcaccggt acggttgtcg ccctggtgct ctactgcgcc
      421 atcagggctg gttttattat tgaagaaaat gaaatgggca tctgacagta ctttaccgtc
      481 ttcacctact gcgaatacgg aagcgtccag atcaaaaccc tgaccatcgg ttacacgggc
      541 atcccagccc aggccaacca tagcgacatt catggttggt gcttctttgg tcagagatac
      601 gttgccgcct tttacgagag aaactgccat ttttagctcc tgcaaacagt tgaatgaggc
      661 tgaattaaca gccccagata tgaaaagtta cttttcgatc aggacgcgtt aatgccgtac
      721 tgagcacata cagatgccag accaccagca taaccctggc ctactgcgcg gaatttccac
      781 tcaccattgt ggcgatacag ctcgccgaac agcatggcag tctcagtgga cgcatcttcg
      841 gtcagatcgt agcgagcgac ttcagtctgg ttatcgtcat taaccagacg aataaacgca
      901 ccggatacct gaccaaagct ctggcgacga gcctgagcat cgtggatggt cacaacgaag
      961 atgatcttgt caacttcaga cgggacggcg tccagtttaa ttttcagcga ttcatcatca
     1021 ccatcgccct caccggtgcg gttatcgccg gtgtgcgtta cggaaccgtc ggatgacgtc
     1081 aggttgttat agaagatgaa atctgaatcg ccgcgcactt tgccgtttga ggccagcagg
     1141 aatgctgaag catccaggtc aaagtcctga ccgtctgttg aacgcgcatc ccagccaagg
     1201 cccaccagga catttttcat tgacggagct gctttactca gggagacgtt cccgcctttg
     1261 gaaagagaaa cactcataaa ataacctctt cgattagtaa ttgttcaggt taacacttaa
     1321 ggggattaac tccccttttc ctcagattca ggtttgcccg ggaacatgac gcttgcgaga
     1381 atgcccagcg ccagtacacc cagtacaaca tacaggctgg ttgttgccgc gatgctgtaa
     1441 ccatgatgcc agatgtgatc ggtcgcattc aggccgagtt ttgccgcgat gaagaacagc
     1501 agcacgataa cggccttctc cagatgaacc aggtactgtt tcagtgcctc aaggacaaaa
     1561 tacagagtac gcaggcccag gatagcaaac atcatggcac tatagacgat aagcggttca
     1621 cgactgacgg caatgattgc cggtaccgag tcaaacgcga acattacgtc cgagagttca
     1681 accacagcca cacacaggaa cagcggggtc gcatacagcg ccgctttttt accacggcca
     1741 atggtgacat cgctgttttc tggtttcgcc agttcagcat ccacttcctt ctggttaagt
     1801 aggaaggcat gccctctgag cttcggccag atagggaaga agcgtttaac catacggtaa
     1861 gccagatgct gggagtaatc ctcaatttca tcatcgtcat caccgctttt aagcatcatg
     1921 accgccgtcc aggcaacgat aatagcgaag acaacttcaa cgtacggccc cagactcagc
     1981 aggctcgtac cgatggcgac aaagatgccc ctgaagacga tggcaccaat gatcccccag
     2041 tagagaacac ggtggcgata acgatccgga acggcgaacc aggagaagat tgccatcatg
     2101 acgaacaggt tatcgaccga cagcactttc tccagcgcat aacccgtgac aaacagactg
     2161 gcaacctcag caccgtggtg gatatagagg aaaccggcaa atgccatcgc aaccacaacc
     2221 cagaatacgg accagagcgc agcactcttc agcgagattg gcttgtcatc acggtgcata
     2281 aacaggtcaa taaagatggc accgactgaa agcgcaataa aaacaatgac cgtttcagtc
     2341 gggaagccga tgtgtgtgga aaccatattt tacccttagg gatgtggatc agaggccaaa
     2401 ttcagccggg tcgaagccca acgcgatagc gatctcgcgg gaggccagtt tctcgtcctg
     2461 atcgaagtta ccgtcacttt tcgcaacggc aataccaaca cgtaaggcca gctgagcggc
     2521 ctcaggctga tctttcagcg ccaggatgta tttcatggtt tcgcccttgc caatttcaac
     2581 atcgaaatcg aagcttgaaa ccagtttatt gaagaactca atcacctcat tggtatcgaa
     2641 gaccttcagc tcttctgaag agcgcagaaa gcccatcatt ttctgctttt cttccgaact
     2701 tacgccgtca ctcgatacgg caatacgggc acatacagca acggtgccct gcatgaattt
     2761 tttgtttttg aaacggccaa cctggcgggt cagttcgtca cggcctgagt taatggcacc
     2821 tttaactttg tcgaaaaagc tcatacctgt ttcctttgtt gctgtgttat ttagaaccgg
     2881 cgctccagcg aaatccccag ccaaatgccc ggtccatttc gtcctggcct ttgaagtact
     2941 ggttaattcg ctcgacttta attgcgccgt tttcgtttac gagtctggca atggcgcaca
     3001 atgtgcgacg gttctcacct tcggtcaggc gggtctcaat gggcggttga tccggcacat
     3061 gaatggtgac tacaccatca gtcttgtccc agctgggaac accttcataa ataaaggcgt
     3121 aaatcagcac ttcgcggata tgcttccatt cacgtccatt gatatgcagc cactcgccgt
     3181 ctgatacatc acccgtccgg tcatcgccct tgagctgaac atacggttca tcgcgataat
     3241 caccgaatgc attaccgagc gcctgaatga ccgatttata cccgtcctga agctcaacaa
     3301 aggcccccag atccaggtcg attcctttgg agccgaacat acctgcaaag cctgatttgc
     3361 tactgccccg gtgccagttc aggttgatcc ggatttcacc gaagttatcc cgtttggtca
     3421 ggctgattgc aggcttctct tttgtcagcg ataccttgct caggctgatt tttgtctcaa
     3481 ctggcggagg ggttacaacc ggaggagttg gggctgcggg agccggttcg tcggcaatat
     3541 ttacgccgaa atgctctgcc agcggtttaa gtccaccgtt gaacccctgc gcaacgaagc
     3601 ggaatttcca gtcattatta cgacggtaaa attcccccag aattaaggct gcttcctgac
     3661 ggccgctcag ttcgacactg ccgctaacca ggccggtagc cccttgctcc acgtcgatgg
     3721 aaagattacg aagaccggac accgtctgcc caccgtcaca ggtcacggtg aacgcaattt
     3781 tctggacatc aggtttcagc cggttgagcg cgacagtaaa ggttgagtac tggccttcac
     3841 tgaccaggct gacggtacca tcatcgttac gtggctgacc ataaaacacc atgtccgcat
     3901 caccctgcac cttcccgtcg gcatacaggc gaaacgccga agcatccacc tggccgcccg
     3961 aggtgatccg gacccgcaac tcctgggctg gaacgggggc attgcccccc ggcgtcaggt
     4021 tcattagcga ataaccgctg aaacgatatc ggagtgcata tcgtcaattg tgcggccgcg
     4081 acaggcatgg ccaagggcgg taaagtccca gttgccgtta ttacggcgca gcgaagaaat
     4141 aacgatcccg gtatgtgagc cctgctcagt cagcttgtag cgagccagct ctttgccggt
     4201 ttgatcgaca acgcggcaga aagcgttttc gacgtcattg aacgactgac cacggaagct
     4261 gtttaccgta aacgccaggt attcaacgtt tgcaggcagg cgggagaggt tgaccttaat
     4321 cacttcatcg tcaccgtcac cctcaccggt caggttatcg ccactgtgca caacagcgcc
     4381 gcaggtggat ttcagtttgc ggaaccagat ggtgtcaatt gttttacctg tgctgtccat
     4441 taatacacag ccagcatcga gatcgattga gtcgttgcct ccaaacaatc ctccaagaag
     4501 accttttttc ttaaccggat cccaaccgag accgaaatga agctggctta atgcagatga
     4561 ttctttgctg agtgataccg tctggttctt aactaatgaa accatgtttt attccttaaa
     4621 atggtaatga ccgagacaat caaaggtata tttagtaact gacgaagaaa atcatatcat
     4681 gatgaagttc tctgacaagg gaaatttttg tggttttctg taaaaacaca aaaagaaatc
     4741 cttttcaggg tgttagtttt tctattttta atttaaatat caatgtgtta taaggaatcc
     4801 aaaaaacact gacgcactcc
//
LOCUS       DAZFWL020000237         4777 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFWL020000237 DAZFWL020000000
VERSION     DAZFWL020000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.238"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..596)
                     /locus_tag="ACHKNP_RS24300"
                     /old_locus_tag="pgaptmp_004860"
     CDS             complement(<1..596)
                     /locus_tag="ACHKNP_RS24300"
                     /old_locus_tag="pgaptmp_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU4706858.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(711..786)
                     /locus_tag="ACHKNP_RS24305"
                     /old_locus_tag="pgaptmp_004861"
     tRNA            complement(711..786)
                     /locus_tag="ACHKNP_RS24305"
                     /old_locus_tag="pgaptmp_004861"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(751..753),aa:Thr,seq:ggt)
     gene            complement(793..867)
                     /locus_tag="ACHKNP_RS24310"
                     /old_locus_tag="pgaptmp_004862"
     tRNA            complement(793..867)
                     /locus_tag="ACHKNP_RS24310"
                     /old_locus_tag="pgaptmp_004862"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Gly,seq:tcc)
     gene            complement(984..1068)
                     /locus_tag="ACHKNP_RS24315"
                     /old_locus_tag="pgaptmp_004863"
     tRNA            complement(984..1068)
                     /locus_tag="ACHKNP_RS24315"
                     /old_locus_tag="pgaptmp_004863"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Tyr,seq:gta)
     gene            complement(1077..1152)
                     /locus_tag="ACHKNP_RS24320"
                     /old_locus_tag="pgaptmp_004864"
     tRNA            complement(1077..1152)
                     /locus_tag="ACHKNP_RS24320"
                     /old_locus_tag="pgaptmp_004864"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1117..1119),aa:Thr,seq:tgt)
     gene            1514..2464
                     /gene="coaA"
                     /locus_tag="ACHKNP_RS24325"
                     /old_locus_tag="pgaptmp_004865"
     CDS             1514..2464
                     /gene="coaA"
                     /locus_tag="ACHKNP_RS24325"
                     /old_locus_tag="pgaptmp_004865"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGU4706859.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2493..3458)
                     /gene="birA"
                     /locus_tag="ACHKNP_RS24330"
                     /old_locus_tag="pgaptmp_004866"
     CDS             complement(2493..3458)
                     /gene="birA"
                     /locus_tag="ACHKNP_RS24330"
                     /old_locus_tag="pgaptmp_004866"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGU4706860.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3455..4483)
                     /gene="murB"
                     /locus_tag="ACHKNP_RS24335"
                     /old_locus_tag="pgaptmp_004867"
     CDS             complement(3455..4483)
                     /gene="murB"
                     /locus_tag="ACHKNP_RS24335"
                     /old_locus_tag="pgaptmp_004867"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGU4706861.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1210 a         1218 c         1086 g         1263 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcatactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaagat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cgcgagcagc accgccgtag
      481 gttttagcca gtacggtagt gattgcagcg gtcagcgttg ttttaccatg gtcaacgtgg
      541 ccgatagtac cgacgttaac gtgcggtttt gtacgttcaa acttttcttt agacatcgat
      601 tgtccctcta agacacggat aaatcggtga tatcaccaca tcaaccaggc aacatgcccg
      661 acttgttgaa tgcaataaac agaaggaaaa aacagggagg agaaaagaag tggtgctgat
      721 aggcagattc gaactgccga cctcaccctt accaagggtg cgctctacca actgagctat
      781 atcagcacat cttggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg
      841 aggtaatagc cattatacga tgcccgcatc ctggaactcg gctacctgat tttcattctg
      901 cactgaatat cgagagaagc tctctttatt cgagccggta agcgaactta tcgtctcggg
      961 ctacgccatc gcgtggccga aattggtggt gggggaagga ttcgaacctt cgaagtctgt
     1021 gacggcagat ttacagtctg ctccctttgg ccgctcggga accccaccgg acttgatggt
     1081 gccgactacc ggaatcgaac tggtgaccta ctgattacaa gtcagttgct ctacctactg
     1141 agctaagtcg gcatcaagta gcgcgcattc tatggagaca tgcgagttca tgcaactaaa
     1201 aaattgcata atttgtttta ttggtcacat tttatgcgac acgatgaaga aacagccgaa
     1261 cggagggcag attagcacac tttttcaaca tcattgtgct caacaatgcg ctcctgctaa
     1321 accataattc tttttatcag atggaatatc tgtcacattg cttttcaacg atagcttcct
     1381 ggcagagatt ttttcttatt attcctcccc atctggtgtt accctcctgc ccattaaccc
     1441 attcaacaga actgtgacgc gccatggcaa atatcgcttt gccgatagag ctatgaccgc
     1501 cagaaacatg cttatgagta taaaagagca aacgttaatg acgccttacc tacagtttga
     1561 ccgcaaccag tgggcagctc tgcgtgattc cgtacctatg acgttatcgg aagatgagat
     1621 cgcccgtctc aaaggtatta atgaagatct ctcgttagaa gaagttgccg agatctattt
     1681 acctttgtca cgtttgctga acttctatat aagctcgaat ctgcgccgtc aggcagttct
     1741 ggaacagttt cttggtacta acgggcaacg cattccttac attatcagta ttgctggcag
     1801 tgtcgcggtg gggaaaagca caaccgcccg tgtattgcag gcgctattaa gccgttggcc
     1861 ggaacatcgt cgtgttgaac tgatcactac agatggcttc cttcacccta atcaggttct
     1921 gaaagaacgt ggtctgatga agaagaaagg cttcccggaa tcgtatgata tgcatcgcct
     1981 ggtgaagttt gtttccgatc tcaaatccgg cgtgccaaac gttacagccc ctgtttactc
     2041 gcatcttatt tatgatgtga tcccggatgg agataaaacg gttgttcagc ctgatatttt
     2101 aattcttgaa gggttaaatg tcttacagag cgggatggat tatccacacg atccacatca
     2161 tgtatttgtt tctgattttg tcgatttttc gatatatgtt gatgcaccgg aagacttact
     2221 tcagacgtgg tatatcaacc gttttctgaa attccgcgaa ggggctttta ccgacccgga
     2281 ttcctatttt cataactacg cgaaattaac taaagaagaa gcgattaaga ctgccatgac
     2341 attgtggaaa gagatcaact ggctgaactt aaagcaaaat attctaccta ctcgtgagcg
     2401 cgccagttta atcctgacga aaagtgctaa tcatgcggta gaagaggtca gactacgcaa
     2461 ataatttgca ggggagcgaa tactcccctt tcttattttt ctgcactacg cagggatatt
     2521 tcaccgccca tccagggttt tattattcca tcctgctcaa gtaataaagc cccctgttta
     2581 tctattccgc gtgaaatgcc aaatatttct ttatcaccaa tgataagttt cactgggcga
     2641 ttaataaaat tatccagctt ttcccagcgc gacagataag gtgccaatcc ttcttgttcg
     2701 aagagttcca acgcagcacg taattcacgt attagcatgg ccgccaacgt attacgatcg
     2761 agattgatcc ccgcttcctg cagcgtgatc cacccctgat taacgacact ctcttcaaca
     2821 cggcgcattg ccatgttgat cccggctcca atgactattt gcgccgcatc gccagttttg
     2881 ccagtcagct ccacaagaat gcctgccagc ttgcgatcct gcagatagag gtcattaggc
     2941 catttgacac gaactttatc tgctcccagc ttgcgtaata cttccgccat cacgataccg
     3001 ataaccagac ttaaaccaat cgccgccgcc gggccttgtt ccagacgcca gaacatcgac
     3061 aaatataagt ttgcgccaaa aggcgaaaac catttccgcc cccggcgacc acggccagcc
     3121 tgctggtatt ctgcaacaca ggcatcgccc gatttaagct ctccgatacg atcaagaagg
     3181 tactgattcg tggagtcaat aactggcagc acggctacac taccgccatc cagctgaccc
     3241 aatatctgtt cagcattaag taactggatg ggctcaggca ggctgtatcc tttacccgga
     3301 acggtaaaga catcaacgcc ccagtcacgc agtgtctgaa tgtgtttatt aatagccgcc
     3361 cggctcattc ccagcgtttc acccaactgc tcgccagagt gaaattcacc gttcgctaac
     3421 agggcaatca atttcagtgg cacggtgtta tccttcatga aattgtctcc actgcgctca
     3481 cttcacctga tgcaccaata aagcggactt caggctcaag ccagacatta aatttttcac
     3541 ccactttttg tcttacatgg tgtgccagtt gcaccacatc ttcgcttttt gcattgtctt
     3601 cattaatgag aactaacgcc tgttgacggt gcaccgcagc cccacccatt tgcatccctt
     3661 ttagctggca ctgatcaata agccaacctg ctgccagttt tactgaacca cccgcctggg
     3721 ggtaatttgg tgctgttgga aattgcgcca gtaatgcttt agccgtttcg gcagatacaa
     3781 cagggttttt gaagaaacta ccggcattgc cattcacttt tggatcaggg agtttggtgg
     3841 tgcgcatatg acacaccgca ttaaatactt gctgtggcgt tactgttgta ggatccagac
     3901 gagttaagtc accatacgtt agtacaggtt gccactcttt tggcagacgc agacctacgg
     3961 ctacaatggc gaagcgatcc tggtattcat gtttaaaaat actgtcgcga tagccaaaac
     4021 ggcactcttt ggcagttaag cgcacttgct tgcctgtcgc cagttcaaca caatcaacat
     4081 aagcgcaaac tcgctgtaat tctacgccat aagcaccaat attctggata ggtgatgagc
     4141 cgacacaacc aggaattaat gccagatttt ccagaccagg cataccttcc tgcaaagtgt
     4201 attttaccag gcgatgccag ttttctccgg ctcctacatg taaataccac gcatcaggtt
     4261 catcatgaat ttcgatacct ttgatccggt tgatgatcac cgtgccgcga tagtcttcca
     4321 gaaaaagtac attacttcct tcacccagaa taagaacggg ttgtccttct gcggttgcat
     4381 actgccaggc attgagtaat tgttgttcgt cttcggcaca tacaatgtgc tgagcattat
     4441 gatcaatgcc aaatgtgttc cagggtttta aggagtggtt catagctgct ttcctgatgc
     4501 aaaaacgagg ctagtttacc gtatctgtgg ggggatggct tgtagatatg acgacaggaa
     4561 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc
     4621 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca
     4681 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4741 aacgaaaggc ccagtctttc gactgagcct ttcgttt
//
LOCUS       DAZFWL020000238         4616 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFWL020000238 DAZFWL020000000
VERSION     DAZFWL020000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.239"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(73..393)
                     /gene="chbB"
                     /locus_tag="ACHKNP_RS24340"
                     /old_locus_tag="pgaptmp_004868"
     CDS             complement(73..393)
                     /gene="chbB"
                     /locus_tag="ACHKNP_RS24340"
                     /old_locus_tag="pgaptmp_004868"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HGU4706862.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(692..1030)
                     /gene="osmE"
                     /locus_tag="ACHKNP_RS24345"
                     /old_locus_tag="pgaptmp_004869"
     CDS             complement(692..1030)
                     /gene="osmE"
                     /locus_tag="ACHKNP_RS24345"
                     /old_locus_tag="pgaptmp_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HGU4706863.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1232..2059
                     /gene="nadE"
                     /locus_tag="ACHKNP_RS24350"
                     /old_locus_tag="pgaptmp_004870"
     CDS             1232..2059
                     /gene="nadE"
                     /locus_tag="ACHKNP_RS24350"
                     /old_locus_tag="pgaptmp_004870"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGU4706864.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2289..3176
                     /gene="cho"
                     /locus_tag="ACHKNP_RS24355"
                     /old_locus_tag="pgaptmp_004871"
     CDS             2289..3176
                     /gene="cho"
                     /locus_tag="ACHKNP_RS24355"
                     /old_locus_tag="pgaptmp_004871"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HGU4706865.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3136..3711)
                     /gene="ves"
                     /locus_tag="ACHKNP_RS24360"
                     /old_locus_tag="pgaptmp_004872"
     CDS             complement(3136..3711)
                     /gene="ves"
                     /locus_tag="ACHKNP_RS24360"
                     /old_locus_tag="pgaptmp_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HGU4706866.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3914..4399)
                     /gene="spy"
                     /locus_tag="ACHKNP_RS24365"
                     /old_locus_tag="pgaptmp_004873"
     CDS             complement(3914..4399)
                     /gene="spy"
                     /locus_tag="ACHKNP_RS24365"
                     /old_locus_tag="pgaptmp_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HGU4706867.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPIMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIVASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1165 a         1105 c         1107 g         1239 t
ORIGIN      
        1 ttaaaaaccg caatttaaat attgcggtat tgatttatga aataactctt tgacgggaaa
       61 atttaaaata aattaatttg ctgcggcttt tttaatcgct gcaaccgcag ccttaagcac
      121 gcctaaacca tcgactttgc cataaagcag cgagtcaatt acttcaaccg gtttgttggg
      181 taacaaacgc tggatttcgg gcaacatata agcaatctgc ggccctaata acacgacatc
      241 ggcattctga cctttttcac cagccagtgt ttccggaaat gcttcaataa tgaccggaac
      301 ttcatatttt tctgcctgtg cgcgcatttt tgataccagt aaagaggtag acatgcccgc
      361 agaacaaaac agataaatgt gtttcttttc cataaaactg ccctcatcga cgattatctg
      421 tcagccagac actccgcaag ccttaacctg cttccatgct ctgggtaact tgcgaaacca
      481 acatgacttt ttttgtggct gaaaggagta tacggtatag acctgagcaa tagtatttct
      541 ttgaccttat aaattgtttc tccttgacct gtggttatga ccctcttcac atttcgggca
      601 aataattcgc gaagataatt aagaactcag gccaaaaaaa accggcgcaa tggccggttt
      661 ccgttgttac tcaagctttc agacgaattg attacttcgc agcctgtgga tcagtgtcgt
      721 attcagcaca ggtctgataa ccggagttga tgacatgtcc ggtatcatct aacgcgacaa
      781 agtaggtttc tgctttacca tcacgttgac ccaggatgta ggtctggcaa gtaccgcgag
      841 catggatcat gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc
      901 tcatgccttt tttgacgtct ttcaccacag gctgtacaaa ctggtctttg gtacgatcat
      961 aagccgtaca acccgccagc atggttaata ccgccgctgc actcagaatt cctgccatat
     1021 tcttgttcat attccgtcct cttgtttatc agcgtgttag ataagcctgg aatacattgg
     1081 gcgctttttc aagcccgtga acgaaacggc tccgctttca gaggattcct gtatgacgtt
     1141 ttaaccacca ttcagcccgc tgtcgcttgt cgtttcagta gcaacgggtt agctttaagg
     1201 aagttttgtc ttttctgtct ggaggggttc aatgacattg caacaacaaa taataaaggc
     1261 gctgggcgca aaaccgcaga ttaatgctga agaggaaatt cgtcgtagtg tcgattttct
     1321 gaaaagctac ctgcaaactt atccgttcat taaatcactg gtgctcggga tcagcggcgg
     1381 tcaggactcc acgcttgccg gaaagctgtg ccagatggcg attaatgagc tgcgccagga
     1441 aactggcaac gaatcactgc aatttattgc cgtacgcctg ccctatggtg ttcaggctga
     1501 cgaacaagat tgccaggatg ccattgcctt tattcaaccg gatcgcgtat taaccgttaa
     1561 tatcaagggc gcggtattgg ctagcgagca ggcattgcgg gaagcaggca ttgaactgag
     1621 cgattttgtc cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat atagcattgc
     1681 gggtatgacc agcggtgtcg tggtgggcac cgatcatgca gcagaagcca ttaccggatt
     1741 cttcactaaa tatggtgacg gcggtacgga tattaatccg ctgtatcgtc tcaacaaacg
     1801 ccagggtaaa cagttgctga cggcattagg ttgcccggaa cacctttata agaaagcccc
     1861 aacggccgat ctggaagatg atcgcccttc tctgccagat gaagtggcac tcggcgtgac
     1921 ctatgacaat atcgacgact atctggaagg gaaaaacgta cctgaacagg tcgccagaac
     1981 aatagagaac tggtatctga aaaccgaaca taaacgccgt ccgccaatta ccgttttcga
     2041 tgatttctgg aaaaagtaat aatttgcaca taatggtttg tgcgaaaaag agtaatttgt
     2101 tcacgccgga tgcggcgtga acgccttatt cgacctacaa aactatgcaa attcaatata
     2161 ttgcaggagt cgaataggcc tgacaggcgt agcacgtcag acggtgtaac ctttgtcatc
     2221 gacccgcctc ttttttaatc gcttcccgcc tgttacactg gatagataac cagcattcgg
     2281 agtcaacagt ggtacggcgt ttaacttctc cgcggctcga atttgaagct gcggcaattt
     2341 atgaatatcc cgaacattta cgttcattcc ttaatgactt acccacccga ccaggggtgt
     2401 atctgtttca tggcgaaagt gacaccatgc cgctctatat cggcaaaagc gttaatatcc
     2461 gcagtcgggt gctttctcat ttacgcaccc cggatgaagc cgccatgcta cggcaatccc
     2521 ggcgaattag ctggatctgc accgcaggcg aaatcggcgc tctgctcctt gaagcgcgat
     2581 taatcaaaga acaacagccg ctgtttaata aacggttgcg ccgcaatcgc cagctctgtg
     2641 ccctgcaatt aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac
     2701 gagcccccaa cctgtttggc ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga
     2761 gcatcgctga tgaacaaaaa ctttgttatg gcctgctggg actggaaccg ttaagtcgcg
     2821 gtcgtgcatg ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga
     2881 gccatgagga acatgcgcta cgcttgcgcc agtctctgga gcgtttgcgg gtggtgtgtt
     2941 ggccttggca aggggcggtg gcgctgaaag aacagcaccc ggaaatgact caatatcata
     3001 ttattcaaaa ctggctgtgg ctgggggcgg ttaattcgct ggaagacgcg acaacgttaa
     3061 ttcggacacc cgccgggttt gatcacgacg gttataaaat tctttgtaag ccgctgcttt
     3121 ccggtaacta tgaaattact gaacttgatc cggcgaatga ccagcgagcc agttgatttc
     3181 actgaacaac agtttacctt gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca
     3241 gcacgcgccc tggtcggtag tgagcagttt gtcgcccagt tgccacgccc cgttaatcac
     3301 aaacactacc ccgccgcgtg agccaaaggt agtaaacgta cgttcggcaa ttctgacttt
     3361 cgccttacag acatccaggc gcgtcataat attgaaatcc atcgacatct gccctgccgt
     3421 cagtttcgct tttaccacct ggtccgccgc aaaggcaaaa ggctgtaacg gttttaaggt
     3481 atggttaaag tggtctgcgc tttcaaggaa catctcgccg ccttccagca acgtcactat
     3541 cctttccata ccgggaaaca aagaaaactc cccattcgcc gcgatggatg caatgctggc
     3601 acgccagtaa aaatcacgtt ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc
     3661 agcatttcgc cacagattca ccgacatttt acgcatatca aagtattcca tactcgctcc
     3721 cggcccgttc ttttcattgt tatcgttcag gtaaccgtgg atggttatcg gcaactcggg
     3781 tgatttacgt tagtggtgat caggcttttt ttacaactgt ttgagaagag aaaagaaaac
     3841 cgccgatcct gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt
     3901 tttggctgaa agattattca gcagttgcag gcattttacc ttttgccgct ggacgttctg
     3961 tcagacgctt ctcaaaatta gcattaaatt gctttttctg ttccggcgtc aggatgttgt
     4021 aaattttgtt ctgggtttcc atgtgcgcca gcatgttagc tttgcgctgt tcttccattt
     4081 ttgcgatctg cgcttcagct tttgctttat cgaaggtatc gctggcaacg atgtcatgca
     4141 ttgcgcggcg ttcttccagc ggcggacgtt tcatctggtc acgctggcct ttcatgattt
     4201 cgcggatctg ctgtttctgc gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat
     4261 gcggaccgaa cttgcctttg tggtgcataa tcggcttcgc gtcagccggt gctgcggtag
     4321 tggtgtctgc ggcatgggcc aggttagccg cgccaagagc cagggtagag gcaacaaaca
     4381 gtgcagttaa tttacgcata ttctatatcc ttcctttcag ttatttatta cggctttctt
     4441 aagtagcgtg ccgtgttgac gagattaact ttactgactt tagcgtcaat taatcagagc
     4501 aacggtaaaa caatgaaagt gtaaaaaaca cttttgcgcc aattatggag aaaaaaagaa
     4561 aatttgatgg agagtgatga gagaatatta caacacgatg attttgcaga gattat
//
LOCUS       DAZFWL020000239         4589 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFWL020000239 DAZFWL020000000
VERSION     DAZFWL020000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.240"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            332..4585
                     /gene="fdeC"
                     /locus_tag="ACHKNP_RS24370"
                     /old_locus_tag="pgaptmp_004874"
     CDS             332..4585
                     /gene="fdeC"
                     /locus_tag="ACHKNP_RS24370"
                     /old_locus_tag="pgaptmp_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HGU4706868.1"
                     /translation="MSHYKTGHKQPRFRYSVLTRCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWHDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     AFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSGQSTVSVAAD
                     RVKAGESTTVTLIAKDAHGNAISGLSLSASLTGAASEGATVSGWTEKGDGSYVATLTT
                     GGKTGELLVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTKLTFTV
                     KDAYGNLVTGLKPDAPQFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     NADASTGQANLQVDTAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
BASE COUNT         1209 a         1073 c         1278 g         1029 t
ORIGIN      
        1 tagttttaat taaaggcagg tcattgttat tcactctgaa tagtgaatta ttcactgtcc
       61 gcagagtaag aaatataact taggtatcta tttaatgact tgcacaaaaa gctaaatttt
      121 cccccataaa taaaaatata atcccgcgcc caaccacctg atgagtggct acaggcactg
      181 gatatattag gtggcggtgc actttcttac ataaaggtat ttccttttct gcggaaaagg
      241 aaatcgggaa atccccggtt tttctgacaa gcagacgcca ttatttgtgt ctgcctatgt
      301 tcgttaattc gttcatcagg aaattatctc aatgtcacat tataaaacag gtcataaaca
      361 accacgattt cgttattcag ttctgacccg ctgcgtggcg tgggcaaata tctctgttca
      421 ggttcttttt ccactcgctg tcacctttac cccagtaatg gcggcacgtg cgcagcatgc
      481 ggttcagcca cggttgagca tgggaaatac tacggtaact gctgataata acgtggagaa
      541 aaatgtcgcg tcgtttgccg caaatgccgg gacattttta agcagtcagc cagatagcga
      601 tgcgacacgt aactttatta ccggaatggc cacagctaaa gctaaccagg aaatacagga
      661 gtggctcggg aaatatggta ctgcgcgcgt caaactgaat gtcgataaag atttctcgct
      721 gaaggattct tcgctggaaa tgctttatcc gatttatgat acgccaacaa atatgttgtt
      781 cactcaggga gcaatacatc gtaccgacga tcgtactcag tcaaatattg gttttggctg
      841 gcgtcatttt tcaggaaatg actggatggc gggggtgaac acctttatcg accatgattt
      901 atcccgtagt catacccgca ttggtgttgg tgcggaatac tggcacgatt atctgaaact
      961 gagcgccaat ggttatatcc gggcttctgg ctggaaaaaa tcgccggatg ttgaggatta
     1021 tcaggaacgc ccggcgaatg gctgggatat tcgtgctgag ggctatttac ccgcctggcc
     1081 gcagcttggc gcaagcctga tgtatgaaca gtattatggc gatgaagtcg ggctgtttgg
     1141 taaagataag cgccagaaag acccgcatgc tatttctgcc gaggtgacct atacgccagt
     1201 gcctcttctg acactgagcg ccgggcataa gcagggcaag agtggtgaga atgacactcg
     1261 ctttggcctg gaagttaatt atcggattgg cgaacctctg gcgaaacaac tcgatacaga
     1321 cagcattcgc gagcgtcggg tactggcagg cagccgctat gacctggttg agcgtaataa
     1381 caacatcgtt cttgagtacc gcaaatctga agtgatccgt attgctctgc ctgaacgtat
     1441 tgaaggtaag ggtggtcaga cactttccct ggggcttgtg gtcagcaaag caactcacgg
     1501 actgaaaaat gtgcagtggg aagcgccgtc attactggct gagggtggca aaattaccgg
     1561 tcagggtagt cagtggcaag taacgctccc ggcttatcgt ccaggcaaag acaattatta
     1621 tgcgatttct gcggttgcct acgataacaa aggcaatgcc tcaaaacgcg tgcagacaga
     1681 ggtggtcatt accggagcag gtatgagcgc cgatcgcacg gcgttaacgc ttgacggtca
     1741 gagccgtatt caaatgcttg ctaacggtaa tgagcaaaga ccgctggtgc tgtctctgcg
     1801 cgacgccgag gggcagccag tcacgggcat gaaagatcag atcaagactg aactagcctt
     1861 caaaccggct ggaaatattg tgactcgttc cctaaaggcc actaaatcac aggcaaagcc
     1921 aacactgggt gagttcaccg aaactgaagc aggggtgtat cagtctgtct ttactaccgg
     1981 aacgcagtca ggtgaggcaa cgattactgt tagcgttgat ggcatgagca aaaccgtcac
     2041 tgcagaactg cgggccacga tgatggatgt ggcaaactcc accctgagcg ctaacgagcc
     2101 gtcaggtgat gtggttgctg atggtcagca agcctatacg ttgacgttga ctgcggtgga
     2161 ctccgagggt aatccggtga cgggagaagc cagccgcttg cgatttgttc cgcaagacac
     2221 taatggtgta accgttggtg ccatttcgga aataaaacca ggcgtttaca gcgccacggt
     2281 ttcttcaacc cgtgccggaa acgttgttgt gcgtgctttc agcgagcagt atcagctggg
     2341 cacattacaa caaacgctga agtttgttgc cgggccgctt gatgcagcac attcgtccat
     2401 cacactgaat cctgataaac cggtggttgg cggtacagtt acggcaatct ggacggcaaa
     2461 agatgctaat gacaaccctg taactggtct caatccggat gcaccgtcat tatcgggcgc
     2521 agctgctgta ggttctacgg catcaggctg gacggataat ggcgacggga cctggactgc
     2581 gcagatttct ctcggcacta cggcgggtga attagaggtt atgccgaagc taaatggaca
     2641 ggatgcggca gcgaatgcgg ctaaagtaac cgtggtggct gatgcgttat cttcaggcca
     2701 gtcgacagtg tctgtagccg cagatcgcgt aaaagcaggc gaaagcacaa ccgtgacgct
     2761 tattgcaaaa gatgcacatg gcaacgctat cagtggtctt tccctgtcgg caagcctgac
     2821 gggtgctgcg tctgaagggg cgactgtttc tggttggacc gaaaaaggtg atggttccta
     2881 tgtcgctacg ctgacaacag gtggaaagac gggtgagctt ctcgtcatgc cgctattcaa
     2941 cggccagcca gcagccaccg aagccgcgca gttgactgtc attgcggggg agatgtcatc
     3001 agcgaactct acgcttgttg ctgacaataa ggctccgacc gtcaaaacga cgacgaaact
     3061 caccttcacc gtgaaggatg cgtacgggaa ccttgtcacc gggctgaagc cagatgcacc
     3121 gcagtttagt ggtgccgcca gcacggggac agagcgacct tcaacaggag actggacaga
     3181 aacaagtaat ggggtctacg tggcgacctt aacgctggga tctgccgcgg gccagttgtc
     3241 tgtgatgccg cgagtgaacg gccaaaatgc cgttgctcag ccactggtgc tgaatgttgc
     3301 tggtgacgca tctaaggctg agattcgtga tatgacggtg aaggttgata accagctggc
     3361 taatggacaa tcgactaacc aggtaaccct gaccgttgtg gacacctatg gtaacccgtt
     3421 gcagggacaa aatgtgacgc tgactctgcc gaaaggtgtg accagcaaga cggggaatac
     3481 ggtaacaacc gatgcggcag gtaaagccga cattgagctg atgtcaacgg ttgccgggga
     3541 acacagcatc acggcctcag tgaataatgc tcagaagacg gtcacggtga aattcaacgc
     3601 ggatgccagc accggtcagg caaacctgca ggtagacacc gctgctcaaa aagtggcaaa
     3661 cggcaaagat gcctttacgc tgacggcgaa cgttgaggat aaaaatggta accctgttcc
     3721 agggagcctg gtgaccttta atctgccccg gggtgtcaag ccgcttacag gcgataatgt
     3781 ctgggtgaaa gccaacgatg aggggaaagc cgagttgcag gtggtttcag tgactgccgg
     3841 aacgtatgag atcacggcat cggcagggaa tagccagcct tcgaatacgc agactataac
     3901 gtttgtagcc gataaggcta ccgcaaccgt ctccggtatt gaggtgatgg gcaactatgc
     3961 gctggcggac ggcaaagcca aacaaacgta taaagttacg gtgactgatg ccaataacaa
     4021 tttggtgaaa gatagcgaag tgacgctgac tgccagcccg gcttcgttaa acctggaacc
     4081 gaatggcact gcgacaacga atgagcaagg gcaggctatt ttcaccgcta ccactactgt
     4141 tgcggcgaca tacacactca aggcgcaagt gagtcagacc aacggtcagg tatcaacgaa
     4201 aactgccgaa tctaaattcg ttgcggatga taaaaacgcg gtactcaccg catcatctga
     4261 tatgcaatct ctggtggcgg atgggaaatc gactgcgaag ctggaggtga cactgatgtc
     4321 ggcaaacaac cccgttggcg ggaatatgtg ggtcgacatt cagacgccgg aaggggtgac
     4381 ggaaaaggat tatcagttcc tgccgtcgaa aaatgaccat ttcgtgagcg gaaaaatcac
     4441 gcgtaaattt agtaccagca agcctggtgt ctatacgttc acatttaacg ccctgaccta
     4501 tggcgggtac gaaatgaagc cagtgacggt gaccattacc gcggtggatg ccgatacggc
     4561 aaaggacgag gaggcgatga aataatttt
//
LOCUS       DAZFWL020000240         4503 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFWL020000240 DAZFWL020000000
VERSION     DAZFWL020000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.241"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..556)
                     /locus_tag="ACHKNP_RS24375"
                     /old_locus_tag="pgaptmp_004875"
                     /pseudo
     CDS             complement(1..556)
                     /locus_tag="ACHKNP_RS24375"
                     /old_locus_tag="pgaptmp_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(516..635)
                     /locus_tag="ACHKNP_RS24380"
                     /old_locus_tag="pgaptmp_004876"
                     /pseudo
     CDS             complement(516..635)
                     /locus_tag="ACHKNP_RS24380"
                     /old_locus_tag="pgaptmp_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(648..2258)
                     /locus_tag="ACHKNP_RS24385"
                     /old_locus_tag="pgaptmp_004877"
     CDS             complement(648..2258)
                     /locus_tag="ACHKNP_RS24385"
                     /old_locus_tag="pgaptmp_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HGU4706869.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSNGSMTLMLKEMSNLINDEGIK"
     gene            complement(2933..3922)
                     /locus_tag="ACHKNP_RS24390"
                     /old_locus_tag="pgaptmp_004878"
     CDS             complement(2933..3922)
                     /locus_tag="ACHKNP_RS24390"
                     /old_locus_tag="pgaptmp_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706870.1"
                     /translation="MTMTKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALALAK
                     AVKTMLNGVLKSAQDNIPFPIEILPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDAKSSTGFTQGTLQQDALNICDNVVRTINSRMV
                     DVLESSKLLKTVELPVLTGSLTAKADTIMDALYENTEPSFGSEVADYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDIAYYNGEDRGVFMMAKRFTALSFGCFRHDGDHITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(3997..>4503)
                     /locus_tag="ACHKNP_RS24395"
                     /old_locus_tag="pgaptmp_004879"
     CDS             complement(3997..>4503)
                     /locus_tag="ACHKNP_RS24395"
                     /old_locus_tag="pgaptmp_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706871.1"
                     /translation="SSKASALFNNQDWKLSVTDINLYENTVSLDGQFYPLSLAIKTLI
                     PGYLSGLPSTSRESMELLEALAEAGVTIGNFFSNDLMTAYGRRQQNKRAEAERIAKEQ
                     RIQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLES
                     GGNWMDNL"
BASE COUNT         1177 a         1039 c          969 g         1318 t
ORIGIN      
        1 aacttcaata tcaacaagac ttctgtaaca tgttttacta aatccaatta caaaatgaat
       61 ggaccaatat aatgattttt tgtctaacac tggagcgttt ccgtattggc agactgtgac
      121 tttatctaac caaagattca attcccctgt tcatattctt attagtggtt gagcagggct
      181 caatactcca tcaaatactt tacctacgtg caaacgatta tattttagtt caggatgctt
      241 tagatttaac ggggaactgg ataaactgag ccattcccct attaaccttt ccgtttctgg
      301 cattaaagcc atcccagcga gctcagatga aattaaggga aatatttctc caagcaatac
      361 ccatccagac cagctgccac tctcttctgc tatcggtaac catttagcac taaattcggg
      421 cattaataat gcaacatctt cgccattttc tttccaattt tgaaaagtgg tttcaaacgt
      481 atgagtgttt cgatttactt tcctcagacc gaacattaac tttcatcctt ctgatcgttt
      541 tcgaatttgc tttcattttc ttgctcctca taaggctgat gtcgttgtcc ttgtcgttca
      601 tcttgctcag tcaataccca acgttgtgct gtaactcatt atgaaaacta tttaattccc
      661 tcatcattga taaggttgga catctctttc aacatgagag tcattgaacc attcgaccaa
      721 tttagcggaa tgatagggct tggcgcttca tgcaaagttc ccgacttgtg gatatagtta
      781 tagctttcgg gaagtgccat ttcaggtaca ctcctgccat ttgcaccaat catattctcg
      841 cctgttattt gggccaatga tctattcata aattgcactg agtcattcaa atactcttcc
      901 tttcttgatt ctttataaac aattgctgct gattttgcat accatgagtc aaaaaaccat
      961 tctgcttctg ttgatggaat gaagctcttt tctcttttcg catgtgaatt ttgatcggta
     1021 tcggttttaa tatcattgaa ccaaaaattt gccgactgat aattatcttt ctcgtatctt
     1081 tttataccat acggacctgc tagtttttta actattttta gaacctgctc ttttcttctg
     1141 gtgtttatct ttgcaagatt tgcaggataa ataacattaa gtaaagccgc atcggcttct
     1201 ctgtaatgtt tatctttttc cagataacct ggggattctc cccctaaatc aagctgtaac
     1261 gttattcttt cataaccttt atctatgagg tggttcaacc tcgttgttga aagaggttca
     1321 tccagttcat tggctttagc ttcgcgtagt aaatcagaga caaaaacaga gtctttcttc
     1381 gagagtaagt tcgacaaacg ttccagtcca gaggtcacca gtgcaacgga agatgtgttt
     1441 aatcgggcat cttcttccca tgctccggaa tcttccataa catagaaatt ggctttatcc
     1501 agataagcaa tcagcagcgc aaccgatttt aatcggtcac ctttttgcca atcttcagcg
     1561 ttaattgtat cagtgtcgat tgcttgtata aggagatcaa gataaagacc taacgcatca
     1621 ttttgtttat ggttccacag ctgcggcttg ccttcttcct gcacatctgc catcacgggg
     1681 gagttgctgt caaatcggat atgcacaaca ttcatttgcc cgggaactcc atccagtcgt
     1741 ttggggttgc taatgacgtc ctgcatccgc ttaatctggt cgggcgtaga catataatcc
     1801 cacagggtga gcagaacctt ttttgcggct acactatttc cctgatctga tactaatgcc
     1861 atatagcccc ataaactatc cctcagccag acagcatcat agtgggtttc tgccgctgtt
     1921 ttgtcatctt cgaaagtaga agcaagaata aacgttcccc agggtttctc cgtcaacgtt
     1981 aactttcgtg tgttttctgt taatgttagc aatccttgaa tttcgtcttt ggtatagtgg
     2041 ctctttattt cattttgtaa tttggctgag tgaatcaaaa gacgataatt aggatactta
     2101 tctggattta acgataaata acttttatct gttgctgccc ccgtggaatt aagaatgtta
     2161 aatacagatt cactcgtcac ttctgcccct gtcgcctggc atgtgccgca cgaaaatata
     2221 ccagcgagca gaagagttaa tgcagatttc cctttcataa aatattccat caatcgtttg
     2281 ttaaaagcgt agataaccac ttatttaaca acttgaaaat aaggtgtttt acctgggttg
     2341 ttacaaaagg attgcattgc gtaaacgctt tttatttaca acaaaatggg gaagtattac
     2401 ggcgattgcg aaaagtctga agcgattgaa ttaagataat atagaaaatc agatacagca
     2461 aaaatggcag attgatcagg tgaagataaa ttggagcggg cgaagggagc ctacgacaaa
     2521 tcgaagacaa ccagctaacc tcttgtttct taaagactga tgaatccagt tggttgtagt
     2581 gatagtcaca catttagcga tagacggtca agacttgaag cacgtaatat tccctctaaa
     2641 ggaatagaac tcacggtctg actacccggc ccttttggtc tgactcaggg tcgggctatg
     2701 gggacttttg gctcgctctt tatattagat gaggtctcac atttttcagt caaaactcat
     2761 cgtctaatag cccatagcca gaccaccagt cccagcttta gcaggaagac cacgagatag
     2821 actttgtgta ggtccatttg gtcctcctcc cttctgtatt cacgaactag attactagta
     2881 caaaataaag gccagacatc cagttcggaa gtctgaccaa tagtcgttat tgttagttag
     2941 cttctttagt gaagctcagg cgtttgacta caggcaggac agcagtagcc gagccagtcc
     3001 ccatcttgat ggtcgtagca gcttcagcta caacaaacac cttgccgaga atctcaaggt
     3061 cgtgagactg ggagtcacca tcacgtgaaa ggaccacagt aatgtggtcc ccatcgtgac
     3121 ggaagcaacc gaagctcaga gccgtaaaac gcttagccat catgaagacg ccacggtctt
     3181 caccgttgta ataggcgatg tcagtcccca acatatcgac gatagcgtct tcaccaccaa
     3241 agccctgctt agcagcaaga cgtgacagtg ccttgaggtg tgactcatgg gcgatgatgc
     3301 cgtaatcagc aacctcagaa ccgaaagaag gctcggtgtt ctcgtacagc gcgtccatga
     3361 tagtgtctgc cttagctgtc aggctaccag tcaggaccgg aagctctaca gtcttcaaca
     3421 gcttggagga ttccagtaca tcgaccatgc gactattgat ggtacggacc acgttatcgc
     3481 agatgttcag agcgtcctgc tggagcgtcc cctgagtgaa accagtggaa gacttagcgt
     3541 cccatagctt ggtctggtgg gacacgagga tagcttgctc aatcggctca acggtgattt
     3601 cagtaccaga cggagcggtc acgacttcag gtcgcttacc accacggaca ggaacgacca
     3661 cagagcggtc agccagagtg tgagcaatga ttggggtcgg taagctattg cgagttggca
     3721 gaatctcaat cgggaatgga atgttatcct gagcggactt cagcacacca ttcagcatgg
     3781 tcttgacagc tttagccagt gccagagcat agacctgtgg gtcagcattg gttggcagac
     3841 cttcgttaac ctgaatgttg tctgtgtcgg tgatacgtac tggctcaatg ccgaactgat
     3901 agctgtcttt agtcatagtc ataataatca atctcctaaa ttagtttaga tacgtgggtc
     3961 ccgacatcag gtcaggacca cttgtgtgcg tctgtcttac aggttgtcca tccagttacc
     4021 gcctgactca aggtcagcga cgatagcggc acgggagcga ccagcggaat gggtagcact
     4081 acgaaggttc tcaccgtagg tccggcgttc agctttgacc tgttcacggc gctgtagctc
     4141 tttctgccac tctgcatcag tcatgttctc ttcacgcatg cgctcggcct gaatgcgctg
     4201 ctctttagcg atacgctcag cctcagctcg cttgttctgc tggcggcgac cataggcggt
     4261 catcaggtca ttactgaaga agttaccaat ggtcacgcca gcttcagcca gggcttccaa
     4321 taactccata gactcgcggc tggtagacgg caggcctgac aggtagcccg gaatcagggt
     4381 cttgatagcg agggacagcg gatagaactg accgtccaga ctcacagtat tctcgtagag
     4441 attgatgtcg gtgactgaca gtttccagtc ttggttattg aataacgcac tagctttgct
     4501 aga
//
LOCUS       DAZFWL020000241         4482 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFWL020000241 DAZFWL020000000
VERSION     DAZFWL020000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.242"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            470..730
                     /locus_tag="ACHKNP_RS24400"
                     /old_locus_tag="pgaptmp_004880"
     CDS             470..730
                     /locus_tag="ACHKNP_RS24400"
                     /old_locus_tag="pgaptmp_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4706872.1"
                     /translation="MNSISVSTPAPAAPVLPVPAQQERFIRLPEVMHLCGLSRSTIYD
                     LISRDAFPQQISLGGKNVAWVQSEVSAWMAARIAAREQGGHA"
     gene            727..1275
                     /locus_tag="ACHKNP_RS24405"
                     /old_locus_tag="pgaptmp_004881"
     CDS             727..1275
                     /locus_tag="ACHKNP_RS24405"
                     /old_locus_tag="pgaptmp_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4706873.1"
                     /translation="MKLALQSHLLFSGLRPDIFSRYSFPAAAKSAAGRRNPSYLLATP
                     DAPCVFFYVVAQAHPFFGLRCLYLSPFQIMVVRAGQPSGWPVSNKAGIPTPVRVTTHE
                     RRNSGGGIYRYLLEVALMATILAPSHPQFIFVFAAVRRAERKPRISMLRIIASNEHHA
                     RLSLVHEYVLCLAARLPVREVI"
     gene            1275..1499
                     /locus_tag="ACHKNP_RS24410"
                     /old_locus_tag="pgaptmp_004882"
     CDS             1275..1499
                     /locus_tag="ACHKNP_RS24410"
                     /old_locus_tag="pgaptmp_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706874.1"
                     /translation="MNHAAISYDDILRLKHLRNVGEFVTGMAVLQDCYEKPASAQCEQ
                     LVSLIYLMTEQLDGVVQRCQDDLMNMEVVQ"
     gene            1496..1819
                     /locus_tag="ACHKNP_RS24415"
                     /old_locus_tag="pgaptmp_004883"
     CDS             1496..1819
                     /locus_tag="ACHKNP_RS24415"
                     /old_locus_tag="pgaptmp_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HGU4706875.1"
                     /translation="MKSRTLSLSLRVALYRRAVACAWLSACYQQNRHLQLTLDEIETA
                     IARELEGFYLRQHGQTKGMEIACALLSDLMEYGPLMSCPALSQLGIAVMDELCVRHIK
                     KPVLH"
     gene            1834..4167
                     /locus_tag="ACHKNP_RS24420"
                     /old_locus_tag="pgaptmp_004884"
     CDS             1834..4167
                     /locus_tag="ACHKNP_RS24420"
                     /old_locus_tag="pgaptmp_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HGU4706876.1"
                     /translation="MSGMKVSQAVKAARGHWAQILPALGVNILKNRHQPCPVCGGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVTRALNVDISEAADRIHGLTHDLSVVNSEVRTL
                     TADTDSGKDAAAALAARLLQASRESAGNTYLTHKGFPEHICHELTSAHKAGGMMFRPG
                     DLIVPLYNADGELVNIQLISGNGSKCFLKGGQVKEAYHLIEGGGSSVRRIWIAEGYAT
                     ALTIHYLTGEAVMVAFSSVNFLSLSSVAHNKYPGYQLIIAADRDLNGSGQNRAETAAK
                     ASQCDIVLPPVFGDWNDALTQYGEESTRKAILEALKPHNASPFDTMSEAEFTAMSVSE
                     KAQRVREHYRDALAVDPNGQLLSRYESGAWKVISQSDFARDVAALFQRLGAPFSSGKI
                     ASLVETLKLIVPQQQNPSRHLIGFRNGVLDTRTGLFSPHCKENWLRTLCEVDFTPPVK
                     GETLETHAPAFWRWLDRAAGHKPAKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIETLESSRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPEQIAPEERD
                     PQLKDKIARELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADPAFDFCGYL
                     EALPDTNGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPVMLKEY
                     GLHYEKRRTNQGMQTNLTLKEESNADWLPKCDEPILK"
BASE COUNT         1091 a         1035 c         1155 g         1201 t
ORIGIN      
        1 ttgcatttca ggaaaatatt cataaaaatg aatttgtctg atgttcatta aacacatccg
       61 gatacatgcg gaagtaagat ggtaatttaa tttctttaat catcggattt attgatacat
      121 atggtgagct gtttattttt ggccgttttg gctcttttat taatcaggtg ttgtcttagt
      181 gcttatgatg taacgccctg aatgatatgg gctggttcag atttgagctg tgatcctgcc
      241 tagtgtaata gactttttta ttattgatgc tccttattat attccataaa ggaattgttt
      301 gtttttgttc gtttcatacg gtttttagct gttttatttt ttgagaaatc aacatttcac
      361 acaaaataaa taaagcatgc gcgagccgct tatataaaca taataaggag ctacctgaat
      421 ccgcatggaa ttgcccggac tgttgtaaac atattagaga ggtagccaga tgaatagtat
      481 ttccgtttcc accccggcac cagctgctcc ggtgctcccg gttccggctc agcaggagcg
      541 cttcatacga ttacctgaag tgatgcacct ttgcgggcta tcccgttcaa ccatctacga
      601 ccttatcagc cgcgatgcct tcccgcagca gatatccctg ggtggaaaaa atgtggcctg
      661 ggtacagtcg gaggtcagtg catggatggc ggcgcgaatt gctgcccgcg agcaaggagg
      721 ccatgcgtga aacttgcttt gcagtctcat cttctttttt ctggcttgcg tcctgatatc
      781 ttttccaggt atagttttcc cgctgccgca aaatcggctg ccgggcgtag gaacccgagt
      841 tacttattgg cgacaccaga cgcgccgtgc gtcttttttt acgtcgttgc tcaggcacac
      901 ccatttttcg ggctgcggtg cttatacctt agcccctttc agataatggt ggtccgggcg
      961 gggcagcctt cgggctggcc ggtttccaat aaggccggta ttcctacccc cgttcgggtc
     1021 accacccatg agcgtaggaa ctccggtggt ggcatttacc gctacttatt ggaggttgcc
     1081 cttatggcta cgatcctcgc cccgtcacat cctcaattta tatttgtttt tgccgccgtt
     1141 cgtcgtgcag aacgtaaacc acgtatcagt atgctgcgta tcatcgccag caatgagcat
     1201 catgcacgtc tttcactggt ccatgagtat gtgctttgtc ttgctgctcg cctgcctgtc
     1261 cgggaggtga tctaatgaat cacgccgcaa tctcttatga cgatattctc cgcctgaagc
     1321 atcttcgtaa cgtgggagag tttgtcactg gaatggctgt tcttcaggat tgctacgaaa
     1381 aacctgccag tgctcaatgt gaacaactgg tttccctgat ttatctgatg acagagcagc
     1441 ttgatggcgt ggtacagcgt tgccaggatg acctcatgaa catggaggtg gtgcaatgaa
     1501 atcacgcact ctttcccttt cacttcgggt agcgttgtat cgccgcgcgg tagcctgcgc
     1561 ctggttgagt gcctgctatc agcaaaaccg ccatctgcag ctcactctgg atgaaattga
     1621 aaccgctatt gcccgtgagc ttgaaggatt ttatctgcga cagcacgggc aaacaaaagg
     1681 catggaaatc gcctgcgccc tgctttcaga cctgatggaa tacggccctc taatgtcctg
     1741 tcccgcactc tcccagctcg gaatagccgt aatggatgag ttatgcgtcc gtcacatcaa
     1801 aaagccggtt ttacactgag ggagaactgc gtcatgtcag gaatgaaagt cagtcaggcc
     1861 gtgaaggctg cccgcggtca ctgggctcag attctgccag cgctgggcgt gaatatattg
     1921 aaaaatcgcc atcagccttg cccggtctgt ggcggtaaag atcgttttcg cttcgacgat
     1981 caggaaggac gcggaacatg gttctgcaat cagtgcggag ccggggatgg tttagcgctt
     2041 gtcaccaggg cactgaatgt ggatatcagt gaagcagctg acaggataca tggactgaca
     2101 catgatctgt ctgtagttaa ttccgaggtc aggacgttaa ccgccgatac cgatagcggg
     2161 aaagatgcag cggcagcact tgccgcgcga ctgctgcaag cctcgcgtga atctgccgga
     2221 aacacttatc ttacacataa aggtttcccg gagcatattt gccatgagct gacttcagct
     2281 cataaggccg gtggtatgat gttccgcccc ggtgatttga tcgtcccact ctataacgct
     2341 gacggggagc tggtgaatat tcagctcatc agtggcaatg gtagcaagtg ttttcttaaa
     2401 ggaggtcagg ttaaggaggc ctatcatctg atagaagggg gcgggagttc tgtaagaagg
     2461 atttggattg cggaaggtta cgccacggcg cttaccattc attatttgac aggagaagcc
     2521 gtcatggtgg cattttcgtc ggtcaacttt ctttctctga gcagcgttgc ccataataag
     2581 tatccgggat atcagttaat tattgcagcg gaccgcgatc tgaatggttc aggccagaac
     2641 agggctgaaa ctgctgcaaa agcgagtcag tgtgacattg tgctgccacc ggtttttggt
     2701 gactggaatg atgcgcttac ccaatacgga gaggaatcca cccggaaggc aattcttgag
     2761 gctttgaagc cacataacgc cagtcctttc gacacaatga gtgaagcaga attcacagcg
     2821 atgagtgtca gcgaaaaagc tcagagggtt cgggagcatt acagggatgc actggcggtt
     2881 gatccgaacg ggcagctttt atcccgctac gagtctggag cgtggaaagt tatttctcag
     2941 tcagattttg cccgagatgt cgcagccctt ttccagcgcc tcggggcacc gttttcctca
     3001 ggaaaaattg cctcactggt ggaaacatta aagttaattg ttccgcagca gcaaaatccg
     3061 tcacgtcatc tgattggttt tcgtaatggc gttctcgata cgcgaacagg gctttttagt
     3121 ccgcactgta aagagaactg gctgcgtacc ctgtgtgaag tcgatttcac gccaccggta
     3181 aagggggaaa cgcttgaaac tcatgccccg gcattctggc gttggctgga ccgggccgcc
     3241 ggacacaagc cagcaaaacg cgacattatt cttgcagcgt tatttatggt gctggcgaac
     3301 cgctatgact ggcagctctt tctggaagtg actggccctg gtggaagtgg caaaagtatt
     3361 ctggctgaaa ttgcgaccat gcttgctgga gaggataacg ccacctctgc gaccattgaa
     3421 acgctggagt cctcgcgaga acgtgcggct ctgataggtt tttctcttat tcgcctgcct
     3481 gatcaggaga agtggagcgg cgacggcgcc gggcttaagg ctataactgg tggcgatgca
     3541 gtatctgtgg accccaaata tcagaatgct tattcaacac atattccggc ggttatcttg
     3601 gccgtaaata ataatcctat gcgcttcact gatcgcagtg gtggtgtgtc ccgcaggaga
     3661 gttattctgc attttcctga gcagatagca ccggaagaac gcgatccgca gttgaaagat
     3721 aaaattgcac gagaactggc tgtgattgtt cgtcagctca tgcagcgttt cagcgatccg
     3781 atgagcgcca gaacattgct tcagtcgcag cagaattctg atgaggctct caccatcaag
     3841 cgtgatgctg atccagcatt tgatttttgt ggctaccttg aggcattacc tgacaccaac
     3901 ggtatgttta tggggaacgc caatattgtc ccacgccagc cacgtacata cctttaccat
     3961 gcctacctcg tatacatgga ggctaatggc tataaaaaca ccctgagtct tacgatgttt
     4021 ggcaaagggt tgccagtcat gctgaaggag tacgggctac attatgagaa acggcgaact
     4081 aatcagggaa tgcaaactaa cctcactttg aaagaggaga gtaatgcaga ctggctgccc
     4141 aagtgtgatg agccaatatt aaaataacga ttaactaacc cggctgatgc cgggcttctt
     4201 attggaaacg cacaaatgta gagatagctg ttcactcttc acctcgctgt taacacgtaa
     4261 gcaattgata tataagttaa aaatggcagg gtgaatagtg tgcatggttt tgcgaaaaaa
     4321 aagtattttt tgtagagtca agtcccggtt tggatccgtt taacggaacc taactagatt
     4381 gactatttct ctgatgattt ttggataaat aatcaacaaa aatcaaaatg tgaacagaga
     4441 atggtggggg cacaaaaagg ggcataattg aattaattat aa
//
LOCUS       DAZFWL020000242         4331 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFWL020000242 DAZFWL020000000
VERSION     DAZFWL020000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.243"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..202)
                     /locus_tag="ACHKNP_RS24425"
                     /old_locus_tag="pgaptmp_004885"
     CDS             complement(<1..202)
                     /locus_tag="ACHKNP_RS24425"
                     /old_locus_tag="pgaptmp_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706877.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALP"
     gene            431..1039
                     /locus_tag="ACHKNP_RS24430"
                     /old_locus_tag="pgaptmp_004886"
     CDS             431..1039
                     /locus_tag="ACHKNP_RS24430"
                     /old_locus_tag="pgaptmp_004886"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HGU4706878.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1137..2528
                     /gene="selA"
                     /locus_tag="ACHKNP_RS24435"
                     /old_locus_tag="pgaptmp_004887"
     CDS             1137..2528
                     /gene="selA"
                     /locus_tag="ACHKNP_RS24435"
                     /old_locus_tag="pgaptmp_004887"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HGU4706879.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYCQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2525..>4331
                     /gene="selB"
                     /locus_tag="ACHKNP_RS24440"
                     /old_locus_tag="pgaptmp_004888"
     CDS             2525..>4331
                     /gene="selB"
                     /locus_tag="ACHKNP_RS24440"
                     /old_locus_tag="pgaptmp_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HGU4706880.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGND"
BASE COUNT          963 a         1112 c         1280 g          976 t
ORIGIN      
        1 cgggcagggc gatgtcggtt tcaccgggaa ggacctttgc accgagcagg ggattgaccg
       61 gatggccgga cgtcactccg ccggggcaca ccgaacaggc cacgccggtg ggggcaccaa
      121 tgcgcacccc ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg tcgccctgac
      181 gcgccgccgg ttttccgctc atctgctcat ccttgtttta tcgggttatt tgtctgcttc
      241 caggcaaaac cctacagatt tataagaaag ccttaagtga gatatccatc agatagaaac
      301 aattaggaaa atatttatat caccatgata aatatgtgat ttatttggaa gtaacaaacc
      361 atcaacattt tctgcccttt ttcccatcac caggccatac tgatattttt cctcgcacaa
      421 aggactcggc atgaaactcg tcggtagcta caccagcccg tttgtacgca aactttctat
      481 tctgttgtta gaaaagggca taactttcga atttattaat gaactgccct ataacgcgga
      541 caacggcgtg gcgcaattta acccgttagg aaaagtgccg gtgctggtga ccgaagaggg
      601 cgaatgttgg tttgattcgc cgatcatcgc tgaatatatt gaattaatga atgtcgctcc
      661 ggcgatgttg ccgcgcgatc cgctggagtc gttgcgggtg cgcaaaattg aggcactggc
      721 ggatggcatt atggatgccg ggctggtatc ggtgcgtgaa caggcgcgtc cagcggcgca
      781 gcagtctgaa gatgaattgt tacgccagcg ggagaaaatc aaccgcagtc tggatgtgct
      841 ggaaggatat ctggtcgatg gcacactcaa aaccgatacg gtcaatctgg cgactatcgc
      901 cattgcctgt gccgtcggat atctcaattt ccgccgcgtt gcgccaggct ggtgtgtcga
      961 tcgcccacac ttagtcaaac tggtcgaaaa cctgtttagc cgcgaaagtt ttgctcgcac
     1021 cgaaccgcca aaggcttgat gcgcgatatg tcctcctgac ccatctcacg ttacaatccg
     1081 tggttatgtt aaacgccctt ctccgtgtga gagggccttg atcagccagg tttcctatga
     1141 caaccgaaac gcgttccctc tatagtcaac ttccggctat tgatcgctta ttgcgcgata
     1201 gctccttcct ttctttgcgt gatacttatg gtcacacccg cgtggtggaa ttgttgcgtc
     1261 agatgctcga cgaagcgcga gaagtgattc gtggcagcca gacgctgcct gcgtggtgtg
     1321 aaaactgggc gcaagaagtc gatgcccggt tgacgaaaga agcgcagagc gcgctgcgtc
     1381 cggtgatcaa cctgacggga accgtgctgc ataccaacct tgggcgagct ttacaggcgg
     1441 aagccgcggt ggaagccgtt gcgcaggcta tgcgttcgcc agtgaccctc gagtacgatc
     1501 tggacgacgc tggacgcgga catcgcgatc gtgcgctggc gcagttgctg tgccgcatta
     1561 cgggggcgga agatgcctgt atcgttaata acaatgcggc ggcggtgtta ttgatgttgg
     1621 cggccactgc cagcgggaaa gaggtggtgg tttctcgcgg cgaactggtg gagattggcg
     1681 gcgcgtttcg tattcccgat gttatgcgtc aggcaggctg caccctacac gaagtaggga
     1741 ccaccaaccg cacgcacgcg aatgattatt gtcaggcggt gaatgaaaat accgcactgt
     1801 tgatgaaagt acataccagt aactacagca ttcaggggtt caccaaagcg atagatgaag
     1861 cggaactggt ggcgctcggc aaagagctgg atgttcccgt agtgactgat ttaggcagtg
     1921 gctcgctggt cgatcttagc cagtacggtt tgccgaaaga gccaatgccg caggagttga
     1981 ttgcggcggg cgtcagtctg gtgagtttct ccggcgacaa gttgttaggc gggccgcagg
     2041 caggaattat tgttggtaaa aaagagatga tcgcccgcct gcaaagccac ccgctgaagc
     2101 gtgcattacg cgcggataaa atgaccctcg cggcgctgga agccacgttg cgtctttatt
     2161 tacaccctga agctctgagt gaaaaattac cgaccctgcg cctgcttacc cgcagcgcag
     2221 aggtcattca aatccaggca caacgtttac aggcccccct tgccgcacat tacggcgcgg
     2281 agtttgcggt acaggttatg ccatgtcttt cgcagattgg cagtggttcg ctgccggttg
     2341 atcgcctgcc gagcgcggca ttaacgttta caccccatga tggacgcggt agccaccttg
     2401 agtcattagc cgcccgctgg cgtgaattgc cagtgccggt gattggtcgt atttatgacg
     2461 gacgattgtg gctggattta cgctgccttg aagatgagca acggtttttg gagatgttgt
     2521 tgaaatgatt attgcgactg ccggacacgt tgaccacggc aaaacaacct tattgcaggc
     2581 gattactggc gtaaatgctg accgtctgcc ggaagaaaaa aagcgcggca tgaccatcga
     2641 tctcggctat gcctactggc cgcagccgga tggtcgcgtg cctggtttta tcgacgttcc
     2701 cggtcatgaa aaatttcttt ccaacatgct ggcgggcgtt ggtggtatcg atcacgcgct
     2761 gttggtggtg gcgtgcgatg acggtgtgat ggcacagacc cgtgagcatc tggcgatttt
     2821 gcagctgacc ggtaacccga tgctgacagt ggtgctgacc aaagccgatc gcgtggacga
     2881 agcgcgtgtt gatgaggttg aacgtcaggt aaaggaggtg ctgcgggaat acggttttgc
     2941 tgaggcaaaa ctgtttatca ccgcagcaac cgaaggtcga ggaattgatg ccctgcgcga
     3001 gcatctgctt cagttaccag aacgtgaaca cgccagccaa catagtttcc gcctcgcgat
     3061 cgaccgcgca tttaccgtaa aaggtgccgg gctggtcgtc accggtacgg cgttaagcgg
     3121 ggaagtgaag gtaggcgatt cactatggct aactggtgta aataaaccga tgcgtgtacg
     3181 tgcgctgcat gcacaaaacc agccaacaga aaccgcccat gccgggcagc gtatcgcgct
     3241 taacatcgcg ggtgatgcgg aaaaagagca gattaaccgt ggcgactggc tgcttgccga
     3301 tgcgccgcca gagccgttca cacgggtgat tgtcgagctt caaacccata cgccgctgac
     3361 ccagtggcag ccgctgcata ttcaccacgc cgccagccac gttacgggac gcgtttcgct
     3421 gctggaagat aaccttgccg agctggtgtt cgacacgccg ctgtggctgg cggataacga
     3481 tcgcttagtg ctgcgcgata tctcggcccg caacacgctg gcgggggcgc gtgtggtgat
     3541 gcttaacccg ccgcgtcgcg gtaaacgtaa gccggaatat ctgcaatggc tggcgtctct
     3601 tgcacgggcg cagagcgatg ccgatgcgtt atctgttcat ctggaacgcg gcgcggttaa
     3661 ccttgcggat ttcgcctggg cgcgccagct caacggcgaa gggatgcgcg aattgctgca
     3721 acagcctggt tatattcagg ctggttatag cttgttgaat gcgccggttg ccgcccgctg
     3781 gcagcggaaa attctcgaca cattagcgac ttatcatgag caacatcgcg atgaacctgg
     3841 tcctgggcgc gaacgtctgc gacgtatggc gttgccaatg gaagatgaag cgctggtact
     3901 gttgctgatt gaaaagatgc gcgaaagcgg cgacatcctc agtcatcacg gttggctgca
     3961 tctgccggat cacaaagcgg gcttcagcga agagcagcag gccatctggc aaaaagcaga
     4021 gccgctgttt ggtgacgagc cgtggtgggt gcgtgatctg gcaaaagaga cgggtaccga
     4081 cgagcaggca atgcgcctga cgctacgcca ggcggcgcag caaggaataa ttaccgcgat
     4141 cgtcaaagat cgttattacc gtaacgatcg gattgtcgaa tttgccaata tgatccgcga
     4201 tctcgatcag gagtgtggtt caacctgcgc ggcggatttc cgcgatcgct taggcgtagg
     4261 ccgaaagctg gcaattcaga ttctggaata ttttgaccgc attggcttta cgcgtcgtcg
     4321 tggaaatgat c
//
LOCUS       DAZFWL020000243         4276 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFWL020000243 DAZFWL020000000
VERSION     DAZFWL020000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.244"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(15..326)
                     /locus_tag="ACHKNP_RS24445"
                     /old_locus_tag="pgaptmp_004889"
     CDS             complement(15..326)
                     /locus_tag="ACHKNP_RS24445"
                     /old_locus_tag="pgaptmp_004889"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4706881.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            525..1223
                     /locus_tag="ACHKNP_RS24450"
                     /old_locus_tag="pgaptmp_004890"
     CDS             525..1223
                     /locus_tag="ACHKNP_RS24450"
                     /old_locus_tag="pgaptmp_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4706882.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGRYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASIIRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1268..2167)
                     /gene="eamA"
                     /locus_tag="ACHKNP_RS24455"
                     /old_locus_tag="pgaptmp_004891"
     CDS             complement(1268..2167)
                     /gene="eamA"
                     /locus_tag="ACHKNP_RS24455"
                     /old_locus_tag="pgaptmp_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HGU4706883.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2362..3549
                     /gene="ydeE"
                     /locus_tag="ACHKNP_RS24460"
                     /old_locus_tag="pgaptmp_004892"
     CDS             2362..3549
                     /gene="ydeE"
                     /locus_tag="ACHKNP_RS24460"
                     /old_locus_tag="pgaptmp_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HGU4706884.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPVNIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            3676..3771
                     /gene="mgtS"
                     /locus_tag="ACHKNP_RS24465"
                     /old_locus_tag="pgaptmp_004893"
     CDS             3676..3771
                     /gene="mgtS"
                     /locus_tag="ACHKNP_RS24465"
                     /old_locus_tag="pgaptmp_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HGU4706885.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3771..3875
                     /gene="mgtT"
                     /locus_tag="ACHKNP_RS24470"
                     /old_locus_tag="pgaptmp_004894"
     CDS             3771..3875
                     /gene="mgtT"
                     /locus_tag="ACHKNP_RS24470"
                     /old_locus_tag="pgaptmp_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HGU4706886.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(3990..>4276)
                     /gene="dgcZ"
                     /locus_tag="ACHKNP_RS24475"
                     /old_locus_tag="pgaptmp_004895"
     CDS             complement(3990..>4276)
                     /gene="dgcZ"
                     /locus_tag="ACHKNP_RS24475"
                     /old_locus_tag="pgaptmp_004895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HGU4706887.1"
                     /translation="YRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITHSEGHIN
                     ITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV"
BASE COUNT         1151 a         1022 c          910 g         1193 t
ORIGIN      
        1 ataatattag attcttactc ttgcagtagt ttcatcgcaa attcaagaac tgcctgtccg
       61 ttcatggtgc cgtagtcttt catctcgata acggctacag gcattttccc ctgtgcaacg
      121 gcttcaatct ctggtttctg aaagcgaact tgcgggccaa gtaaaacaac gtcaacttcg
      181 ttgtttttaa ttttcccttt agcctctgca atcgccaatg cggaaatatt aacttcaagt
      241 gaaatagcgt tagcatgatc aatcatacgt ttaaccagca tactggttga catgcccgca
      301 gcgcaaacta acaatatttt tttcatattt taccttactg gtatataaca gattacattg
      361 tttaaatatc atatcccgca gtagctatac gtcctggtac attgatcaca gtaatacagt
      421 gataaagttg ttcaacatca gggtgattat taaacgactc ggccatacga taaaccaatc
      481 aggactcaac tccttgtttt caatggctct taaaaggtgc aaaagtgatt tttcaaaaga
      541 ttgcccgctt acttaaatcc gaaatcaatg gcaattcatg gcatgttggt gatttgttgc
      601 cgtcagaagc ggaactcgct gttcgttata atgtttcgcg taataccctg cgtaaggcat
      661 tatccttgct ggaaggcgaa ggaattattc acagaaagca tggttcagga agatacattc
      721 aaaaaaagaa ttttgttgca cacattgatc acatgaacag tttcagtgaa attgcgcata
      781 aaagcggcaa agaggcaggg agtcagatta tgaaatttga agtgcaggat gcttcaccca
      841 ctattgcgac tgagctgaat ttagtgactg gtgagcaggt ttattacata aagagactgc
      901 gattcattga ggataatgca gcacaattag aagaaacgtg gatgtcagta gcacgttttc
      961 ctgatttaac cgtgtcacat atgcaaaaat ccaaattttc atatattgag aatgaatgcg
     1021 ggatcaaaat cattggcacc tttgaaactt tctcccctac ttttcctacc ccagaaatcg
     1081 ccagtattat acggatcagc ccacgggatc ccatacttaa aattcagacc caggctgtgg
     1141 acagtaactc tattccgctg gattattcgt tactttacag caatattttc gagttccagg
     1201 taaagtactt tttcccgcga taaaagcaga cgcacagagt gctgacatca cattctgtgc
     1261 gtatggttta gcctctaacc tttaccgctt tacgccaccg caagccaaat acattgatat
     1321 acagcccggt cataatgagc accgcaccta aaaattgcag acccgttaag cgttcatcca
     1381 acaatagtgc cgcacttgcc agtccgacta cgggcaccag taacgataac ggtgcaaccc
     1441 gccaggtttc atagcgtccc agtaacgtcc cccagatccc ataaccaaca attgtcgcca
     1501 caaacgccag atacatcagc gacaagatgg tggtcatatc aatagtaacc agactgtgaa
     1561 tcatggatgc ggaaccatcg agaatcagcg aggcaacaaa gaagggaatg attgggatta
     1621 aagcgctcca gattaccagc gacatcaccg ccggacgcgt tgagtgcgac atgatctttt
     1681 tattgaagat gttgccacac gcccaactaa atgctgcagc cagggtcaac ataaagccga
     1741 gcatcgccac atgctgaccg ttcagactat cttcgattaa caccagtacg ccaaaaatcg
     1801 ctaaggcgat ccccgccaat tgtttgccat gcagtcgctc cccgaaagta aacgcgccaa
     1861 gcacgatagt aaaaaacgcc tgtgcctgta acaccagcga agccagtcca gcaggcatac
     1921 caaagttaat ggcgcagaac agaaaggcaa actgcgcaaa actgatggtt aatccatacc
     1981 ccaagagcaa attcagtggt actttcggtc gtgcaacaaa aaagatggcc ggaaaagcga
     2041 ccagcataaa gcgcaaaccg gccagcatca gcggtggcat gttatgaagc cccactttga
     2101 tgaccacaaa atttagcccc catacgacca ctaccagtag cgccaacacc ccatcttttc
     2161 gcgacattct accgcctctg aatttcatct tttgtaagca atcaacttag ctgaatttac
     2221 ttttctttaa cagttgattc gttagtcgcc ggttacgacg gcattaatgc gcaaataagt
     2281 cgctatactt cggatttttg ccatgctatt tctttacatc tctaaaacaa aacataacga
     2341 aacgcactgc cggacagaca aatgaactta tccctacgac gctctaccag cgcccttctt
     2401 gcctcgtcgt tgttattaac catcggacgc ggcgctacgc tgccatttat gaccatttac
     2461 ttgagtcgcc agtacagcct gagtgtcgat ctaatcggtt atgcgatgac aattgcgctc
     2521 actattggcg tcgtttttag cctcggtttt ggtatcctgg cggataagtt cgacaagaaa
     2581 cgctatatgt tactggcaat taccgccttc gccagcggtt ttattgccat tcctttagtg
     2641 aataacgtga cgctggtggt gctctttttt gcccttatta actgcgccta ttccgttttt
     2701 gctaccgtgt tgaaagcctg gtttgccgac aatctttcgt ccaccagcaa aacgaaaatc
     2761 ttctcaatca actacaccat gctaaacatt ggctggacca tcggtccgcc gctcggcacg
     2821 ctgttggtga tgcagagcat caatctgccc ttttggctgg cagctatctg ttccgcgttt
     2881 cccatgcttt tcattcaaat ttgggtaaag cgcagcgaga aaatcatcgc cacggaaaca
     2941 ggcagtgtct ggtcgccgaa agttttatta caagataaag cactgttgtg gtttacctgc
     3001 tctggttttc tggcttcctt tgttagcggc gcatttgctt catgcatttc acaatatgtg
     3061 atggtgattg ctgatggaga ttttgccgaa aaggtggtcg cggttgttct tccggttaat
     3121 gctgccatgg tagttacgtt gcaatattcc gtgggccgcc gccttaaccc ggttaacatc
     3181 cgcgcgctga tgacagcagg caccctctgt ttcgtcatcg gtctggttgg ttttattttt
     3241 tccggcaaca gcctgctatt gtggggtatg tcagctgcgg tatttactgt cggtgaaatc
     3301 atttatgcgc cgggcgagta tatgttgatt gaccatattg cgccgccagg aatgaaagcc
     3361 agctattttt ctgcccagtc tttaggctgg cttggtgctg caattaaccc attagtgagt
     3421 ggcgtagtgc taaccagcct gccgccttcc tcgctgttta tcatcttagc gttggtgatc
     3481 attgctgcgt gggtgctgat gttaaaaggg attcgtgcaa aaccgtgggg gcagcccgcg
     3541 ctttgttgat ttaagtcgaa cacaataaaa atttaattca gccttcgttt aggttacctc
     3601 tgctaatatc tttctcattg agatgaaaat taaggtaagc gaggaaacac accacaccat
     3661 aaacggaggc aaataatgct gggtaatatg aatgttttta tggccgtact gggaataatt
     3721 ttattttctg gttttctggc cgcgtatttc agccacaaat gggatgacta atgaacggag
     3781 ataatccctc acctaaccgg ccccttgtta cagttgtgta caaggggcct gatttttatg
     3841 acggcgaaaa aaaaccgcca gtaaaccggc ggtgaatgct tgcatggata gatttgtgtt
     3901 ttgcttttac gctaacaggc attttcctgc actgataacg gatcgttgac acagtagcat
     3961 cagttttctc aatgaatgtt aaacggagct taaactcggt taatcacatt ttgttcgtca
     4021 ataaacatgc agcgatttct tccggtttgc ttaccctcat acattgcccg gtctgctctt
     4081 ccaatgacca catccagagg ctcttcagga aatgcgcgac tcacacctgc tgtcacggta
     4141 atgttgatat gcccttcaga atgtgtgatg gcatggttat cgactaactg gcaaattctg
     4201 acacctgcac gacatgcttc ttcatcatta gccgctttga caataatgat aaattcttcg
     4261 cccccgtagc gataaa
//
LOCUS       DAZFWL020000244         4253 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFWL020000244 DAZFWL020000000
VERSION     DAZFWL020000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.245"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            170..1273
                     /gene="proB"
                     /locus_tag="ACHKNP_RS24480"
                     /old_locus_tag="pgaptmp_004896"
     CDS             170..1273
                     /gene="proB"
                     /locus_tag="ACHKNP_RS24480"
                     /old_locus_tag="pgaptmp_004896"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGU4706888.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1285..2538
                     /gene="proA"
                     /locus_tag="ACHKNP_RS24485"
                     /old_locus_tag="pgaptmp_004897"
     CDS             1285..2538
                     /gene="proA"
                     /locus_tag="ACHKNP_RS24485"
                     /old_locus_tag="pgaptmp_004897"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGU4706889.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2653..2728
                     /locus_tag="ACHKNP_RS24490"
                     /old_locus_tag="pgaptmp_004898"
     tRNA            2653..2728
                     /locus_tag="ACHKNP_RS24490"
                     /old_locus_tag="pgaptmp_004898"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2686..2688,aa:Thr,seq:cgt)
     gene            2892..4100
                     /locus_tag="ACHKNP_RS24495"
                     /old_locus_tag="pgaptmp_004899"
     CDS             2892..4100
                     /locus_tag="ACHKNP_RS24495"
                     /old_locus_tag="pgaptmp_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4706890.1"
                     /translation="MKLNARQVDAAKPREKAYKLADGAGLYLEVVPSGSRYWRMKYRF
                     NGKEKRMAFGVYPAVSLAQARALRDDAKKKLAEGIDPSLAKKEEKLVRDVQLNNTFQA
                     VALEWHGTKVSRWSEGYASDIIEAFNKDIFPYIGQQPVNEIKPLVLLNVLRRIESRGA
                     TEKAKKVRQRCSEVFRYAIVTGRAEYNPAADLTSAMSGHESKHYPFLTVEELPDFFKA
                     LAGYTGSPLVVLAARLLILTGVRTGELRGAFWSEFDLEKAVWEIPAERMKMKRPHLVP
                     LSTQALEIVQQLKVMSGQYPLVFPGRNDPRKTMSEASINQVFKRIGYTGRVTGHGFRH
                     TMSTILHEEGFNTAWIETQLAHVDKNAIRGTYNHALYLEGRREMMQWYADYINANHNS
                     CISLLVNATK"
BASE COUNT         1052 a         1013 c         1176 g         1012 t
ORIGIN      
        1 tgtggtaatt tattaaatct gtaataaaag cgtaaacaac tgccgctagg cttgctgatc
       61 ccgcgcaaca aaacgccatg ctttgctcgc agatggttgg caaccgacga cagtcctgct
      121 aaaacgttcg tttgatatca tttttcctaa aattgaatgg cagagaatca tgagtgacag
      181 ccagacgctg gtggtaaaac tcggcaccag tgtgctaaca ggcggatcgc gccgtctgaa
      241 ccgtgcccat atcgttgaac ttgttcgcca gtgcgcgcag ttacatgccg ccgggcatcg
      301 gattgttatt gtgacgtcgg gcgcgatcgc cgccggacgt gagcacctgg gttacccgga
      361 actgccagcg actatcgcct cgaaacaact gctggcagcg gtagggcaga gtcgactgat
      421 tcaactgtgg gaacagctgt tttcgattta tggcattcac gtcgggcaaa tgctgctgac
      481 ccgtgctgat atggaagacc gtgaacgctt cctgaacgcc cgcgacaccc tgcgtgcgtt
      541 gctcgataac aatatcgttc cggtaatcaa tgagaacgat gctgtcgcta cggcagagat
      601 taaagtcggc gataacgata acctttctgc gctggcggcg attctggcgg gtgccgataa
      661 actgttgctg ctgaccgatc aaaaaggttt gtataccgct gacccgcgca gcaatccgca
      721 ggcagaactg attaaagatg tttacggcat tgatgacgca ctgcgcgcga ttgccggtga
      781 cagcgtttca ggcctcggaa ctggcggcat gagtaccaaa ttgcaggctg ctgacgtggc
      841 ttgccgtgcg ggtatcgaca ccattattgc cgcgggcagc aagccgggcg ttattggtga
      901 tgtgatggaa ggcatttccg tcggtacgct gttccatgcc caggcgactc cgcttgaaaa
      961 ccgtaaacgc tggattttcg gtgcgccgcc ggcgggtgaa atcacggtag atgaaggggc
     1021 aactgccgcc attcttgaac gcggcagctc cctgttgccg aaaggcatta aaagcgtgac
     1081 tggcaatttc tcgcgtggtg aagtcatccg catttgcaac ctcgaaggtc gcgatatcgc
     1141 ccacggcgtc agtcgttaca acagcgatgc attacgccgt attgccgggc accactcgca
     1201 agaaattgat gcaatactgg gatatgaata cggcccggtt gccgttcacc gtgatgacat
     1261 gattacccgt taaggagcag gctgatgctg gaacaaatgg gcattgccgc gaagcaagcc
     1321 tcgtataaat tagcgcaact ctccagccgc gaaaaaaatc gcgtgctgga aaaaatcgcc
     1381 gatgaactgg aagcacaaag cgaaatcatc ctcaacgcta acgcccagga tgttgctgac
     1441 gcgcgtgcca atggccttgg cgaagcgatg cttgaccgtc tggcactgac gcccgcacgg
     1501 ctgaaaggca ttgccgatga tgtgcgccag gtgtgtaacc tcgccgatcc ggtggggcag
     1561 gtaatcgatg gcagcgtact ggacagcggc ctgcgtcttg agcgtcgtcg cgtaccgctg
     1621 ggggttattg gcgtgattta tgaagcgcgc ccgaacgtga cggttgatgt cgcttcgctg
     1681 tgcctgaaaa ccggtaatgc ggtgatcctg cgcggtggca aagaaacgtg tcgcactaac
     1741 gctgcaacgg tggcggtgat tcaggacgcc ctgaaatcct gcggcttacc ggcgggtgcc
     1801 gtgcaggcga ttgataatcc tgaccgtgcg ctggtcagtg aaatgctgcg tatggataaa
     1861 tacatcgaca tgctgatccc gcgtggtggc gctggtttgc ataaactgtg ccgtgaacag
     1921 tcgacaatcc cggtgatcac aggtggtata ggcgtatgcc atatttacgt tgatgaaagt
     1981 gtagagatcg ctgaagcatt aaaagtgatc gtcaacgcga aaactcagcg tccgagcaca
     2041 tgtaatacgg ttgaaacgtt gctggtgaat aaaaacatcg ccgatagctt cctgcccgca
     2101 ttaagcaaac aaatggcgga aagcggcgtg acattacacg cagatgcagc tgcactggcg
     2161 cagttgcagg caggccctgc gaaggtggtt gctgttaaag ccgaagagta tgacgatgag
     2221 tttctgtcat tagatttgaa cgtcaaaatc gtcagcgatc ttgacgatgc catcgcccat
     2281 attcgtgaac acggcacaca acactccgat gcgatcctga cccgcgatat gcgcaacgcc
     2341 cagcgttttg ttaacgaagt ggattcgtcc gctgtttacg ttaacgcctc tacgcgtttt
     2401 accgacggcg gccagtttgg tctgggtgcg gaagtggcgg taagcacaca aaaactccac
     2461 gcgcgtggcc caatggggct ggaagcactg accacttaca agtggatcgg cattggtgat
     2521 tacaccattc gtgcgtaaat aaaaccgggt gatgcaaaag tagccatttg attcacaagg
     2581 ccattgacgc atcgcccggt tagttttaac cttgtccacc gtgattcacg ttcgtgaaca
     2641 tgtcctttca gggccgatat agctcagttg gtagagcagc gcattcgtaa tgcgaaggtc
     2701 gtaggttcga ctcctattat cggcaccatt tcaaactctt cccaagtcta ccgaaatcaa
     2761 ctaaaagctc gtatactgcg gtttctggcc cgtatcttat ctcctgttat caactagact
     2821 caaccgaaat caagtcgcag ttgggggcat aagtgggggc attttgtgtt cggtctaggg
     2881 agatgccccc aatgaaactt aatgcgcgac aggtagacgc agctaaaccc agagagaaag
     2941 cctataagct ggcagatggt gctggtttgt atcttgaggt tgttccctct ggttccagat
     3001 actggcggat gaaatatcgc ttcaatggaa aagagaagcg tatggccttt ggtgtctatc
     3061 cggcagtgtc ccttgcacaa gcgagggcac tgcgtgacga cgccaagaaa aagcttgccg
     3121 aaggtatcga tccatcgctt gccaagaaag aagaaaagct ggttcgagat gtgcagctaa
     3181 ataatacgtt tcaggctgtg gcacttgaat ggcatggaac gaaggtgagc cgatggtcag
     3241 aaggttatgc ctcggacatt atcgaagcct ttaataaaga tatttttccc tatattggcc
     3301 aacaaccggt gaatgaaatc aaaccgctgg ttctgcttaa tgtgctgcgt cgaattgaaa
     3361 gccgtggcgc gacagagaag gccaagaagg ttcgccagcg ttgcagcgaa gtctttcgtt
     3421 acgccatcgt aaccggtcgt gcggaataca atcctgcagc ggatcttacc agcgcgatgt
     3481 cagggcatga atcgaagcat tatcccttcc ttactgttga ggagttacca gactttttta
     3541 aagctctcgc aggctacaca ggaagcccgt tagttgttct tgcggcacgt ctgctgatcc
     3601 tcacgggagt tcgcactggc gaactccgag gtgctttctg gagtgagttt gatcttgaaa
     3661 aagcggtgtg ggagatacct gccgagcgta tgaagatgaa acggcctcac cttgtccccc
     3721 tctctaccca agcgctggaa atcgtacagc aactcaaagt gatgtcaggg caatatccac
     3781 tggtgttccc gggacgtaat gatccccgca agacgatgag tgaagcgagt attaatcagg
     3841 tttttaagcg gattggatat acggggaggg taacggggca tggtttccgt cacacgatga
     3901 gtacgatttt gcatgaggaa ggtttcaata cagcgtggat tgaaacccag ctggctcacg
     3961 ttgataaaaa tgcgattcgt gggacgtata accatgcgtt gtatttggaa ggacgtaggg
     4021 agatgatgca gtggtatgcg gactatataa acgctaatca taattcatgt atttctttgc
     4081 tggtgaacgc tacaaagtag atttgacgtg atgataggat tgattaggaa aatgacctaa
     4141 aaccgtatat tttgatactt aagtttaaac attagcccta tatccgatcg ccctttgtta
     4201 cctacacttc tatctttttt aggttctagg gctgaaagat ttgaagataa gtt
//
LOCUS       DAZFWL020000245         4113 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFWL020000245 DAZFWL020000000
VERSION     DAZFWL020000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.246"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..437
                     /gene="cesT"
                     /locus_tag="ACHKNP_RS24500"
                     /old_locus_tag="pgaptmp_004900"
     CDS             <1..437
                     /gene="cesT"
                     /locus_tag="ACHKNP_RS24500"
                     /old_locus_tag="pgaptmp_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HGU4706891.1"
                     /translation="AEKIGIGSISFNENRLCSFAIDEIYYISLSDANDEYMMIYGVCG
                     KFPTDNPNFALEILNANLWFAENGGPYLCYESGAQSLLLALRFPLDDATPEKLENEIE
                     VVVKSMENLYLVLHNQGITLENEHMKIEEISSSDNKHYYAGR"
     gene            498..3317
                     /gene="eae"
                     /locus_tag="ACHKNP_RS24505"
                     /old_locus_tag="pgaptmp_004901"
     CDS             498..3317
                     /gene="eae"
                     /locus_tag="ACHKNP_RS24505"
                     /old_locus_tag="pgaptmp_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type beta"
                     /protein_id="HGU4706892.1"
                     /translation="MITHGFYARTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENY
                     FKLSSDSKLLTQNAAQDRLFYTLKTGETVANISKSQGISLSVIWSLNKHLYSSESEMM
                     KAGPGQQIILPLKKLSVEYSALPVLGSAPVVAAGGVAGHTNKMTKMSPDATKSNTTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGMASSQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSENMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHGGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLPNGQVVDQVGVTDFTAD
                     KTSAKADGIEAITYTATVKKNGVAQANVPVTFSIVSGTATLGANSARTDGNGKATVTL
                     KSATPGQVVVSAKTAEMTSPLNASAVIFVDQTKASITEIKADKTTAKADGSDAITYTV
                     RVMKEGAPVVDQKVTFSKDFGTLNKTEATTDQNGYATVKLSSNTPGKAIVSAKVSGVG
                     TEVKATTVEFFAPLSIDGDKVTVIGTGITGALPKNWLQYGQVKLQATGGNGKYTWKSS
                     NTKIASVDNSGVITLNEKGSATITVVSGDNQSATYTINAPGSIVIAVDKNTRVTYFDA
                     ENKCKTNSANLAQSKELLANIYSTWGAANKYPYYSGSKSLTAWIKQSSSEQSSGVSST
                     YDLVTKNQLINVGVNNKNAFSVCVK"
     gene            complement(3532..>4113)
                     /gene="escD"
                     /locus_tag="ACHKNP_RS24510"
                     /old_locus_tag="pgaptmp_004902"
     CDS             complement(3532..>4113)
                     /gene="escD"
                     /locus_tag="ACHKNP_RS24510"
                     /old_locus_tag="pgaptmp_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HGU4706893.1"
                     /translation="ISAINDILTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVEN
                     LLLNTPGVAGWEIHNNSNNKINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNES
                     KILAIINAMNKNSNVKILFQNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIG
                     SILKGGYVIDAIDPKDGINISRPDEYIHIPLSY"
BASE COUNT         1217 a          763 c          903 g         1230 t
ORIGIN      
        1 ttgcagaaaa aattggtatt ggatctattt catttaatga aaatagattg tgttcttttg
       61 ctattgatga gatttattat atttcgttat ctgatgctaa tgacgaatat atgatgatct
      121 atggcgtctg tgggaaattc ccgacagata atcctaactt cgctcttgag attttgaatg
      181 caaacttatg gtttgcagag aatggtggac catacctgtg ttatgaatca ggagcacaat
      241 cgctgttgtt agcgttacgt tttccgctcg atgatgctac acctgaaaaa cttgagaatg
      301 aaatagaagt cgtcgttaag tcaatggaaa acctgtattt ggtattacat aatcagggaa
      361 taacattaga aaacgaacat atgaaaatag aggaaattag ttcaagcgat aataaacatt
      421 attacgccgg aagataaaag attatttatt aatataattt actattcatt ctaactcatt
      481 gtggtggagc ccataacatg attactcatg gtttttatgc ccggacccgg cacaagcata
      541 agctaaaaaa aacatttatt atgcttagtg ctggtttagg attgtttttt tatgttaacc
      601 agaattcatt tgcaaatggt gaaaattatt ttaaattgag ttcagattca aaactgttaa
      661 ctcaaaatgc cgctcaggat cgcctttttt atacgttaaa aacaggtgaa actgttgcca
      721 atatttctaa atcacagggt atcagtttat cggtaatttg gtcactgaat aaacatttat
      781 acagttccga aagcgaaatg atgaaggctg gacctggtca gcagatcatt ttgccactca
      841 aaaaactgtc tgttgaatat agtgccttac ctgtcttagg ttcggcacct gttgttgctg
      901 caggtggtgt cgctggtcat acgaataaaa tgactaaaat gtccccggac gcgactaaaa
      961 gcaacacgac cgatgacaag gctctaaatt atgcggcaca acaggccgcg agccttggta
     1021 gccagctcca gtcgcgctca ctgaacggcg attacgcgaa agataccgct cttggtatgg
     1081 ccagcagcca ggcttcgtca cagttgcagg cctggttaca acattatgga acggcagagg
     1141 ttaatctgca gagtggtaat aactttgacg gtagttcact ggacttctta ttaccgttct
     1201 atgattccga aaacatgctg gcatttggtc aggtcggtgc gcgttacatt gactcccgct
     1261 ttacggcaaa tttaggtgct ggccagcgtt ttttccttcc tgaaaatatg ttgggctata
     1321 acgtcttcat tgatcaggat ttttctggtg ataatacccg tttaggtatt ggtggcgaat
     1381 actggcgaga ctatttcaaa agtagcgtta acggctattt ccgcatgagc ggctggcatg
     1441 agtcatacaa taagaaagac tatgatgagc gcccggcaaa tggttttgat atccgcttta
     1501 atggctattt accatcatat ccggcattag gcgccaaact gatgtacgaa cagtattatg
     1561 gtgataatgt tgctttgttt aattccgata agttgcagtc gaatcctggc gcggcgaccg
     1621 ttggtgtaaa ctacactccg attcctctgg tgacgatggg gatcgattac cgtcatggta
     1681 cgggtaatga aaatgatctc ctttactcaa tgcagttccg ttatcagttt gataaaccgt
     1741 ggtctcagca aatcgagcca cagtatgtta acgagttaag aacattatcg ggcagccgtt
     1801 acgatctggt tcagcgtaat aacaatatta ttctggagta caaaaagcag gatattcttt
     1861 ctctgaatat tccgcatgat attaatggta ctgaacacag tacgcagaag attcaattga
     1921 tcgttaagag caaatacggt ctggatcgta tcgtctggga tgatagcgca ttacgcagtc
     1981 agggcggtca gattcagcat ggcggaagcc aaagcgcaca agactaccag gctattttgc
     2041 ctgcttatgt gcaaggcggc agcaatattt ataaagtgac cgctcgcgcc tatgaccgaa
     2101 atggtaatag ttctaataat gtacagctca ctattaccgt tttaccgaat gggcaggttg
     2161 tggaccaggt tggggtaacg gactttacgg ctgataaaac atcggctaaa gcggatggca
     2221 tagaagctat tacctatacc gcgacggtta aaaagaatgg tgtagctcag gctaatgtcc
     2281 ctgtaacatt tagtattgta tccgggactg caactcttgg ggcaaatagt gccagaacgg
     2341 atggtaacgg taaggcgacc gtaacgctga agtcggctac gccaggacag gtcgtcgtgt
     2401 ctgctaaaac cgcggagatg acttcgccac ttaatgccag cgcggttata tttgttgatc
     2461 aaaccaaggc cagtattact gagattaagg ctgataaaac aacagcgaag gcagatggtt
     2521 ctgatgcgat tacctatact gtcagagtga tgaaggaggg ggcacccgta gtagatcaga
     2581 aagtgacctt ttctaaggat tttgggaccc tgaataagac tgaagcaaca accgatcaga
     2641 atggttatgc tactgtaaaa ttatcatcca atactcctgg caaggccatt gttagtgcaa
     2701 aagtgagtgg agtaggtaca gaagttaagg ctactaccgt tgagtttttt gccccgttga
     2761 gtattgatgg tgataaagtg accgtaattg gtactggtat cacgggggct ctgccaaaga
     2821 actggttaca gtatggtcag gttaagctac aggcaacagg gggcaatgga aaatacacat
     2881 ggaaatccag taatactaaa attgcttctg ttgataactc gggagtgata accttaaatg
     2941 aaaaagggag tgccacaatt actgtagtat ctggtgataa tcagagtgcg acatacacaa
     3001 ttaatgcacc gggtagtatt gtaattgctg tggataaaaa tactcgagtt acgtattttg
     3061 atgccgaaaa caaatgtaag acaaatagcg caaatttagc acagtcaaaa gaactattgg
     3121 ccaatatcta ttcaacatgg ggtgctgcaa ataaatatcc ttactattct ggttctaaat
     3181 cattgactgc ttggattaaa caatcctctt ctgaacagtc atcaggtgta tcaagcacat
     3241 atgatttggt tacgaagaac cagttgatca atgttggagt aaacaataag aatgcttttt
     3301 ctgtttgtgt aaaataattt aattacatct caaatcccgg aaaataaaat tcattttccg
     3361 ggatttttta ttgtacctac ttgaatcttt taataaacgt ttcatgttaa aatcaatgtc
     3421 tcatttcgga ataagttttt cgtgtatctt gtttgataga gaccttgttt accatatcta
     3481 caacatctgc acaataaaaa accctccgaa gagggggaag ataataataa gttaataaga
     3541 caacggaata tgtatatatt cgtctgggcg ggagatgttg ataccatctt taggatcaat
     3601 cgcgtctatg acatagcctc ctttcagaat gctaccaatt cccaggcttg taccgttatc
     3661 aagagcaatt gttggattct tcatcgtgcc gctgattctg agtatttttc cggggaaaat
     3721 atctgctgaa atgtacggct gaatgttctg aaatagtatc ttgacgttac tatttttgtt
     3781 catcgcattt ataatggcaa ggatcttact ctcgttctgt tgagaaacct cccccgcaac
     3841 aatgatgaca tcattctttt tgaaaatatt gacatagttt attaatttat tctttttgaa
     3901 ctctgatgcc agttcattga ttttattgtt actgttgtta tgtatttccc atccagcaac
     3961 ccctggcgta ttcagtaaca gattttcaac ttcgctccat tttggtgaag ggggaatgta
     4021 tcccgataac aggaagaatc caggcttatt accttttgta atgatgatat ccttataacc
     4081 gtattccgtt aaaatatcat taatcgcgga gat
//
LOCUS       DAZFWL020000246         4100 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFWL020000246 DAZFWL020000000
VERSION     DAZFWL020000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.247"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(77..412)
                     /locus_tag="ACHKNP_RS24515"
                     /old_locus_tag="pgaptmp_004903"
                     /pseudo
     CDS             complement(77..412)
                     /locus_tag="ACHKNP_RS24515"
                     /old_locus_tag="pgaptmp_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            342..671
                     /locus_tag="ACHKNP_RS24520"
                     /old_locus_tag="pgaptmp_004904"
     CDS             342..671
                     /locus_tag="ACHKNP_RS24520"
                     /old_locus_tag="pgaptmp_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU4706894.1"
                     /translation="MLDTQELAPVAIALLLSVIGGIGTFLMDVRDGRQSGNLLGLVTE
                     IFVAVTAGAVAYLLGQHEGWELSITYLMVTIASNNGHEVISGMKRVNIDSILNVLTSL
                     VKKGGGK"
     gene            668..1111
                     /locus_tag="ACHKNP_RS24525"
                     /old_locus_tag="pgaptmp_004905"
     CDS             668..1111
                     /locus_tag="ACHKNP_RS24525"
                     /old_locus_tag="pgaptmp_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4706895.1"
                     /translation="MIGWGVCALALALADRYLLKRKDITHLELGDVEIKPGFIRVPFK
                     YRSKFPFLRGATVRYWIRDVQKPTTVIEGEQRCLTSAEQGENSEWLYIPTEYMGKGER
                     LWHFNVMVTHGDSFINPLYRIFPVTQQIRRSYVINLAQDVSDDEK"
     gene            1098..1700
                     /locus_tag="ACHKNP_RS24530"
                     /old_locus_tag="pgaptmp_004906"
     CDS             1098..1700
                     /locus_tag="ACHKNP_RS24530"
                     /old_locus_tag="pgaptmp_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="odaE"
                     /protein_id="HGU4706896.1"
                     /translation="MTKNKYATVDFDQVNEKGLKSLIAAINKTGVTVIEVDSSNRATT
                     KDGVKVKTAKLVLNDGQILAIQVNDTGDISSVRLNGKAIPNAQSPDIKTLGTVMGQAA
                     RKNSAKFQKSLIAKAKRVANPVDKKPAVKSNFQRLQEAKQRNAQVVAAYKSAQNSVSF
                     NQQQITDLRAKLDKETGRLNNEKARNGELKRRLKQLKAGN"
     gene            1702..3621
                     /locus_tag="ACHKNP_RS24535"
                     /old_locus_tag="pgaptmp_004907"
     CDS             1702..3621
                     /locus_tag="ACHKNP_RS24535"
                     /old_locus_tag="pgaptmp_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706897.1"
                     /translation="MEQFNINKGVTIKPGLDVLPPPVTDDEYRALMAGEDRYLMTESN
                     TLEEIEATFFYDTPIHWCATDLLEAISSTRLQLHRTMQAFVRALNQKLNGTGISAGSD
                     KTGDVAQSGARAIGGAEIGRARNVNGLSVLPAIIPLSDGQTISILFHSPTAENRITNS
                     DTLVAFQFLLNKKDVTHTVAPMSGRDMTLAQVTMKLANLAEKNSAKFQRAQKKKKALV
                     DEITQLQADSDQKEDAMSDLVDQVAAVEGQKVDLEQKINAVASEADSLYEENERLQTE
                     IDQLNRTGGRETIAPAGMTGGHSRAMTDRLASIKNRMHMNGEVTLSNGASMKQFIEDG
                     EGYIQLTDSDGSVYMIKAKSIQGVDMADAIGKLFKAYKAGNVSEYLVQPEEHKPENVE
                     PEPAEDTGSSSPEPEVSVGAYRYALQMRPAAPGAIPEGNKAILPRPDEGDPYYEYARY
                     GIATYDTPLSDQQMSEYDLKLLPREDSFDFLAKTLTNGPFGKYAQKALELATSSPDEF
                     RVMLKTQFQKTFPNIAYPGGAGTEKMVQSMINALQAEVGEITQPEPAPAQPDETVSEA
                     DAEANKAIEYLNNVMDMQSTDMAEIRNARGNVREAIAALQAAGRFEENEELVNGAARH
                     LADLLVAIQKAGVAA"
     gene            3618..3983
                     /locus_tag="ACHKNP_RS24540"
                     /old_locus_tag="pgaptmp_004908"
     CDS             3618..3983
                     /locus_tag="ACHKNP_RS24540"
                     /old_locus_tag="pgaptmp_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ddrA"
                     /protein_id="HGU4706898.1"
                     /translation="MTLSAIELMDLSDKLDALMSKAATASGMELLDISDEIDQIMQQM
                     GYGVSGGSSDEEKQPSEHDGVPKLIADFLADKFVDQSTDAFIGTLQDLSQYVGTYIDL
                     DQVKQHTAAWIAANIKEAA"
     gene            complement(4023..>4100)
                     /locus_tag="ACHKNP_RS24545"
                     /old_locus_tag="pgaptmp_004909"
     CDS             complement(4023..>4100)
                     /locus_tag="ACHKNP_RS24545"
                     /old_locus_tag="pgaptmp_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706899.1"
                     /translation="LACGGMVQSGRPLKQEPTEMIQATA"
BASE COUNT         1113 a          923 c         1109 g          955 t
ORIGIN      
        1 aggaaattaa caaaattgtt agctcttatt tgttgagctt atagatatta attaaggccc
       61 gattccgggc ctttcctcat tccggctttt cgggaaacgt tactggaagg atggaggtat
      121 ccgtggattc gactttctgc gcatagagaa tccactcggt taatttttgt ttaccctgaa
      181 cggaaataat gcctagccgt aactgtgagt cccagagctg agttttatcc ctgacgagct
      241 gtagcaggct ttgcttttct ttttccgctt gttgtctttg catgaccggt tgagaagtta
      301 ctttgcatac cattacctcc tgacaacgta ggagggaact tgtgcttgac acacaggaat
      361 tagctccagt tgctattgcg ctcctgcttt cagtaattgg tgggataggc acgttcctga
      421 tggatgtccg agacggtcgc cagtctggca atttgttggg attggttacg gagatctttg
      481 ttgcagtgac agctggcgcg gtggcgtacc tattggggca acacgagggc tgggagttat
      541 caattacgta cttaatggta acgatagcca gcaataacgg tcatgaggtg atttcaggga
      601 tgaaacgagt gaatatcgat agcattctga atgttcttac aagtttggtg aaaaagggag
      661 gtgggaaatg attggctggg gtgtatgcgc tcttgcgtta gccttagccg atcgctattt
      721 gctaaaacgc aaggacatca cgcatttaga acttggtgat gtggaaatta aaccgggttt
      781 catccgggtg ccgttcaaat accggtctaa attcccgttt ttgcgtggcg caacggtcag
      841 atattggatc cgcgatgttc agaagccaac gacagtgatt gaaggcgaac aacgttgtct
      901 gacgtcggct gaacagggcg aaaacagtga atggttgtac atacccactg aatatatggg
      961 taaaggagaa cggctgtggc atttcaacgt catggttacg catggcgact cgttcattaa
     1021 cccgttgtat cggattttcc ctgttactca gcaaatccgc agaagttacg taataaatct
     1081 cgcacaggat gtgtcagatg acgaaaaata agtatgcaac ggtcgatttt gaccaggtta
     1141 atgaaaaggg gctgaaatcc cttatcgcgg cgatcaataa aaccggtgtt acggtaattg
     1201 aggttgactc cagcaaccgc gcaacaacga aagatggcgt taaagttaaa accgcaaagc
     1261 tggttcttaa cgacggacaa attcttgcca tacaggtaaa cgatactggc gatatatcgt
     1321 ctgtgaggct gaatggaaaa gctattccta acgctcaatc gccggatatc aagacgcttg
     1381 gtaccgtcat ggggcaggcg gcccgcaaaa actccgcaaa attccagaaa tcactgatcg
     1441 ccaaagcgaa acgtgttgcc aatccggtag acaagaaacc ggcagtaaaa tccaactttc
     1501 agcgcctgca agaggcaaaa cagcggaatg ctcaggtggt tgccgcttat aaatcagcgc
     1561 agaactcggt gtctttcaat caacagcaga tcactgattt gcgggcgaag ctggataagg
     1621 agacaggccg actcaataac gaaaaggccc gaaatggcga actcaaacgc cgtcttaagc
     1681 aactgaaagc aggaaattaa catggaacag ttcaatatca ataaaggggt gacgatcaag
     1741 cctgggcttg acgtgcttcc cccgccagtg actgatgatg aatatcgcgc attaatggcc
     1801 ggtgaggacc gctatctgat gacggaatcc aacaccctgg aggaaatcga ggctacgttc
     1861 ttctatgaca cgccgatcca ctggtgtgct acggatttac tggaggcgat tagttctact
     1921 cgtttgcagt tacaccggac catgcaggca tttgtccggg cattgaacca gaagctgaat
     1981 ggtaccggaa tctctgcggg gagtgataaa acgggggatg tggcccagag cggcgcgcgc
     2041 gcgatcggcg gagctgaaat tggtcgggca cgtaacgtta acgggttgtc ggtcctgcca
     2101 gccattattc cgctcagtga tggtcagact atcagcattt tgtttcatag cccgacagcg
     2161 gaaaaccgga tcaccaatag cgatacgctg gttgctttcc agttcttact gaataaaaaa
     2221 gacgttactc acaccgttgc tccgatgagt ggacgtgata tgacgctggc gcaggtcacc
     2281 atgaaacttg ccaaccttgc agagaaaaac tcggcaaaat ttcagcgtgc gcagaagaag
     2341 aaaaaagccc ttgttgatga aataacccaa ctacaggctg acagtgacca gaaagaggat
     2401 gccatgagcg acctcgtgga tcaggtggca gcggtagaag ggcagaaggt agatctggag
     2461 cagaaaatta acgctgttgc atcggaagcg gattctcttt atgaagagaa tgagcgtttg
     2521 cagacggaga ttgatcagct caatcgaact ggtgggcgcg aaaccattgc tcctgcgggg
     2581 atgactggtg gacactctcg cgcgatgacg gatcgccttg caagtatcaa aaatcgtatg
     2641 catatgaacg gggaagtgac gctcagtaat ggtgcatcaa tgaagcaatt cattgaggac
     2701 ggtgaagggt atatccagtt aaccgattcg gatggcagcg tgtacatgat caaggctaaa
     2761 tccatacagg gtgtggacat ggcagatgcg atcggcaagc tgtttaaagc ctataaagcg
     2821 ggtaatgtat cggaatacct ggtccaacca gaagaacata aaccggaaaa cgtcgaacct
     2881 gaaccagcgg aggataccgg tagctcttcg cctgaaccag aagtctctgt aggtgcatat
     2941 cgatatgccc tgcaaatgcg tccggcggcc cctggcgcaa tacctgaagg taacaaagca
     3001 attctgccgc gccctgatga aggtgacccg tattatgaat atgcacgcta cggcattgct
     3061 acttacgata ccccgctttc tgatcagcaa atgagtgagt acgacctgaa gttattgcct
     3121 cgcgaggatt ctttcgactt cctggcgaag acacttacta atggtccgtt tggcaaatat
     3181 gcacaaaaag ctctggagct ggccaccagc tcaccagacg agttccgcgt aatgctgaaa
     3241 actcagtttc aaaaaacttt ccccaatatt gcgtatccgg ggggcgctgg caccgagaaa
     3301 atggtgcaga gcatgatcaa tgcattgcag gccgaagtcg gtgagattac tcagccagaa
     3361 cctgccccgg cacagcctga tgaaacggtt agcgaagcag atgcagaggc taataaagcc
     3421 attgaatatc tcaataacgt gatggatatg caaagcactg acatggcgga gatccgtaac
     3481 gcccggggta atgtccggga agcgattgca gcccttcagg ctgccgggcg ttttgaggaa
     3541 aacgaagagc tggttaatgg cgctgctcgc cacctggctg atctgctggt agcaatccag
     3601 aaagcggggg tagcggcatg acactatcag ctattgagtt aatggatctc agcgataagt
     3661 tggatgctct gatgtccaaa gcggctaccg cgagtggcat ggagttgctg gatatcagcg
     3721 atgaaattga ccagatcatg caacagatgg ggtacggcgt gtccggcggc agtagtgacg
     3781 aggaaaaaca accttcggaa catgatggtg tgccaaaact gattgctgat ttcctggctg
     3841 ataaattcgt cgatcagagc actgatgcat ttatcggtac gttacaggac ttgagtcaat
     3901 atgttggcac atacatcgac ctggaccaag ttaaacagca cacggcggca tggatagccg
     3961 ccaacattaa agaggcagcg tgacatcctc cacgcccttc ggggcgtgga ttcctgctac
     4021 gttcaggctg tcgcctgaat catttcggtg ggttcctgct tcaacgggcg gcctgactgc
     4081 accatccctc cacaggcaag
//
LOCUS       DAZFWL020000247         4062 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFWL020000247 DAZFWL020000000
VERSION     DAZFWL020000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.248"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            16..198
                     /locus_tag="ACHKNP_RS24550"
                     /old_locus_tag="pgaptmp_004910"
     CDS             16..198
                     /locus_tag="ACHKNP_RS24550"
                     /old_locus_tag="pgaptmp_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGU4706900.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            171..362
                     /locus_tag="ACHKNP_RS24555"
                     /old_locus_tag="pgaptmp_004911"
     CDS             171..362
                     /locus_tag="ACHKNP_RS24555"
                     /old_locus_tag="pgaptmp_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU4706901.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
     gene            439..654
                     /locus_tag="ACHKNP_RS24560"
                     /old_locus_tag="pgaptmp_004912"
     CDS             439..654
                     /locus_tag="ACHKNP_RS24560"
                     /old_locus_tag="pgaptmp_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU4706902.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            753..974
                     /locus_tag="ACHKNP_RS24565"
                     /old_locus_tag="pgaptmp_004913"
     CDS             753..974
                     /locus_tag="ACHKNP_RS24565"
                     /old_locus_tag="pgaptmp_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU4706903.1"
                     /translation="MADIIDSASEIEELQRNTAIKMHRLNHQAISATHCCECGDPIDE
                     LRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            971..1276
                     /locus_tag="ACHKNP_RS24570"
                     /old_locus_tag="pgaptmp_004914"
                     /pseudo
     CDS             971..1276
                     /locus_tag="ACHKNP_RS24570"
                     /old_locus_tag="pgaptmp_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            1573..1923
                     /locus_tag="ACHKNP_RS24575"
                     /old_locus_tag="pgaptmp_004915"
     CDS             1573..1923
                     /locus_tag="ACHKNP_RS24575"
                     /old_locus_tag="pgaptmp_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4706904.1"
                     /translation="MINRTKLEHILEYARQQRRFGQLCKILPGDMVEIVEIAMRKTGN
                     SPVSPGGWISCSERMPAQDDWILIYSKHGEYMAGQVQEEYVELSDGTLSWLGNALYWM
                     LLPEPPQGVNDELA"
     gene            1998..2240
                     /locus_tag="ACHKNP_RS24580"
                     /old_locus_tag="pgaptmp_004916"
     CDS             1998..2240
                     /locus_tag="ACHKNP_RS24580"
                     /old_locus_tag="pgaptmp_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4706905.1"
                     /translation="MFALIQRGQIYTDSAGYPIKILRCINNTVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQENHLKKLRAMQRR"
     gene            2359..2703
                     /locus_tag="ACHKNP_RS24585"
                     /old_locus_tag="pgaptmp_004917"
     CDS             2359..2703
                     /locus_tag="ACHKNP_RS24585"
                     /old_locus_tag="pgaptmp_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706906.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            2809..3027
                     /locus_tag="ACHKNP_RS24590"
                     /old_locus_tag="pgaptmp_004918"
     CDS             2809..3027
                     /locus_tag="ACHKNP_RS24590"
                     /old_locus_tag="pgaptmp_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4706907.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTSSLLKRIRNGKKAKS"
     gene            3005..>4062
                     /locus_tag="ACHKNP_RS24595"
                     /old_locus_tag="pgaptmp_004919"
     CDS             3005..>4062
                     /locus_tag="ACHKNP_RS24595"
                     /old_locus_tag="pgaptmp_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4706908.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSGNRRESLIDRIKGADAITLHAWLDRYETILSERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLADISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCR
                     MKWSDINDNHLHIEQGKTGAKLAIPLTLTIDALNISLADTLQKCREASGSETIIASTH
                     HEPLSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDSMAARYRDSRGREWDK"
BASE COUNT         1210 a          893 c         1051 g          908 t
ORIGIN      
        1 tggggagcaa tggtaatgaa gcatcctcac gataatatcc gggtaggcac gatcactttc
       61 gtctactccg ttacaaagcg aggctgggta tttcccggcc tttctgttat ccgaaatcca
      121 ctgaaagcac agcggctggc tgaagagata aataataaac gaggggctgt atgcacaaag
      181 catctcctgt tgaattaaga acgagtatcg ggatggcaca tagcctcgct caaattggag
      241 tcaggtttgt gccaatacca gtagaaacag acgaagaatt tcatacgtta gccacatccc
      301 tttcacaaaa gctggaaatg atggcggcga aagcagaagc aaacgagaga gacccggcat
      361 gacaacaaca gaatgcattt ttctggcagc aggcttcata ttctgtgtgc ttatgcttgc
      421 cgacatggga cttgttcaat gacacctcag caggaaaacg cccttcgcag cattgcccgt
      481 caggctaatt ctgaaatcaa aaaagccaga cagcagtttc cggataaaaa cgtcgatgac
      541 atttgccgta gcgtactgaa gaagcaccgc gaaacggtaa cgctaatggg attcacaccg
      601 actcatttaa gtctggcgat cggcatgtta aacggcgtct ttaaggagcg atgaacatga
      661 aaagcaaaat catcagggag ctacaggctc cttttttatt gttcgcattc accctcaagc
      721 gtattaacca acaattcagg gattaatgga agatggcaga catcattgat tcagcatcag
      781 aaattgaaga attacagcgc aacacagcaa taaaaatgca ccgcctgaac caccaggcta
      841 tatctgccac tcattgttgt gagtgtggcg atccgataga tgaactaaga cgtctggccg
      901 ttcagggttg tcggacttgt gcaagttgcc aagaggatct ggagcttatc agtaaacaga
      961 gaggttcgaa gtgagcgaaa ttaactctca ggcactgcgt gaagcggcag agcaggcaat
     1021 gcatgacgac tggggatttg atgcggacct tttccatgag ctggtaacac catcgattgt
     1081 gctggcactg ctggatgaac gggaaagaaa ccagcaatac atcaaacgcc gcgaccagga
     1141 gaacgaggaa attgcgctaa cggtagggaa gctgcgtgtt gagcttgagg aagcaaaatc
     1201 aaaactcaac gagcagcgtg agtattacga aggtgttatc tcggatggga gtaaacgtat
     1261 tgctgaactg gagaaaagcg aagagcaact cattaacgag cgtgaccatg ctgagtctgc
     1321 tttagctgat atgtacttcg caacaaccgg ggataggcct gagtggagta actgtttcag
     1381 tttttcagat gctgtcgatg ccgtagttga cagaattgct gatttagaag ccaaacagcc
     1441 atcgccagta gtaccggaag aaaaaccaat gcctaaccct cttagcatgt acacagttga
     1501 tgctgttgca gctattgcag aggtgagagg ctggaacgcc tgccgtgcag ccatgcttaa
     1561 gggagataaa tcatgattaa tcgaaccaaa ctggagcaca tcctcgagta tgccaggcag
     1621 cagaggcgct ttggccagct ttgtaaaatt ctgccaggag atatggttga aatcgtggag
     1681 attgccatgc gtaagactgg caactctccg gtaagtccgg gtggttggat aagctgtagt
     1741 gagcgaatgc ccgctcaaga tgattggatt ttaatttatt caaagcacgg tgagtatatg
     1801 gcaggacagg tacaagagga atacgtggag ttgagcgacg gcactttatc gtggttaggg
     1861 aacgccttgt actggatgct gctaccagaa ccgccgcagg gagtgaatga tgaattggcc
     1921 tgaagcattc accgctgtag gagttgcaat cgcggtggca tttattctgt attcgctttt
     1981 ccgctgggga taaaggaatg ttcgctctga ttcaacgtgg tcagatatac accgatagcg
     2041 ccggctaccc gataaaaatt cttcgctgca taaacaacac tgtgttgtac agaagaatgg
     2101 atgggcgaac acagtcggta aaaataaacg attttaatga actgtttgaa cggatcgatc
     2161 accaggaata ccgacaaatt ctggctgaaa cagagcagga gaaccatctg aaaaaattac
     2221 gcgccatgca aaggagataa accggtaaag gtgttcgcga taaaggtgaa tatcggcaat
     2281 gaataacaat cctcgcactc gcggggattt cttttatctg aactcgctac ggcgggtttt
     2341 gttttatgga gacaagaaat gtcagatttg gctatgaagg ttttgaaatg gcaatcgact
     2401 ggcgatgttg gcatcagtag cgcaactctt gcctcaatcg catgtggact gaaaaagaat
     2461 atctatggtc atcacttcgg cgctccacat gacgcagcag acttccggcg atgcgttgca
     2521 cttgttgagc agattccaga aatcagagat tcattcgaca aggttgcaaa gcgcgttccg
     2581 gcattcaaag gcatcctcaa cgaatgggat tccctcgtcg ctctgttgaa gtctgaaatg
     2641 aagatacacg gaaacaaagc accagagact tacagaagaa ttagcgagct acgcaaggac
     2701 taaccacagc ctcacactca atgaggcctg ttcatttctc aagatatcca gacctaccat
     2761 tgccgcatca atgcggcttt tcttgcgtgt aattgcggag actttgcgat gtacttgaca
     2821 cttcaggagt ggaatgcacg ccagcgacgc ccaagaagcc ttgaaacagt tcgtcgatgg
     2881 gtgcgcgaat gcaggatatt ccctcctccg gttaaggatg gaagagagta tctgttccac
     2941 gaatcagcgg taaaggttga cttaaatcga ccagtaacaa gtagcctttt gaagaggatc
     3001 agaaatggga agaaggcgaa gtcatgagcg ccgggattta ccccctaacc tttatataag
     3061 aaacaatgga tattactgct acagggaccc aaggacgggt aaagagttcg gattaggccg
     3121 agacaggagg atagcaatca ctgaagcaat acaggccaat attgagttgc tatccgggaa
     3181 caggcgtgag tcactgatag acagaattaa aggcgctgac gcaatcactc ttcatgcgtg
     3241 gcttgaccga tatgaaacaa tcctcagcga gaggggtatc aggccgaaaa ctctactcga
     3301 ctacgccagc aaaatcaggg caatccgaag aaaattgccg gacaaaccgc tcgctgacat
     3361 atcaacgaaa gaggtggcag caatgctaaa cacctacgta gcagaaggta aagcggcttc
     3421 cgcaaaatta atcaggtcaa cccttgttga cgtttttcgt gaggcaatag ccgaggggca
     3481 tgtggctacg aatccggtaa cagcaacccg cacagcaaaa tcagaagtaa ggcgttcaag
     3541 actgacagct aatgaatatg tcgcgattta ccatgcagcc gaaccgctcc caatctggct
     3601 gaggctggca atggatttgg ctgtcgttac agggcagaga gtaggcgatt tatgcagaat
     3661 gaaatggtca gacataaacg acaaccatct tcacattgag cagggtaaaa caggggctaa
     3721 gctcgccatt ccgctgacgc taacgattga cgcgctcaat atctcattgg ctgatacact
     3781 acagaaatgc agggaggcca gcggcagtga aacaataatt gcatcaacgc atcacgaacc
     3841 gctttccccg aaaacagtat ctaagtattt tacaaaggcg agaaatgcat ctggactctc
     3901 atttgatgga gacccgccaa cattccatga actgcgcagc ctgtcggcga ggctataccg
     3961 gaatcagatt ggcgataagt ttgctcaacg tcttctcggg cataaatcag attcaatggc
     4021 ggcgcgttac agggacagtc gcgggcggga gtgggacaaa at
//
LOCUS       DAZFWL020000248         4059 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFWL020000248 DAZFWL020000000
VERSION     DAZFWL020000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.249"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(34..1029)
                     /locus_tag="ACHKNP_RS24600"
                     /old_locus_tag="pgaptmp_004920"
     CDS             complement(34..1029)
                     /locus_tag="ACHKNP_RS24600"
                     /old_locus_tag="pgaptmp_004920"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HGU4706909.1"
                     /translation="MSYSEIVKKYENISKSTNYSIDILDKLEDDLQKIFSEKNLEFTM
                     VTTGSFGRLEASEESDIDLFIFCKKESEKHYILYKKEEIEACIKKYIHKKAGDTGTFG
                     VDAVDVFDNILKNIGGGDDTNKSLTRRMLFLLEGKAIYNKNIFNSYKKKLITKYLNSS
                     SEGKLDKYLLNDVIRYYRTITTDFQHKVDADGKSWGIRNIKLRYSRKLLYFAGVMAIA
                     HASETQQDITKRTQEISNSLDIPPLERIYNISHGKAKPELIEDIFCQYEFFLSCISDP
                     EKRASLNDIKDKSDRLNSPLYNQLSEKSTEFTDNLYKLLNNAFEKNNQIHTSLIF"
     gene            complement(1058..2086)
                     /locus_tag="ACHKNP_RS24605"
                     /old_locus_tag="pgaptmp_004921"
     CDS             complement(1058..2086)
                     /locus_tag="ACHKNP_RS24605"
                     /old_locus_tag="pgaptmp_004921"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HGU4706910.1"
                     /translation="MGDFFKRNDIDEEIEKESIGYWIQDLQKSSKLLMIIKTAAFNRL
                     YGISFLGAIDYSDQNELTKKERNRAIHSLYVAGIANYVATKRNYNSDLKEHLVAAALL
                     HDIGHMPLSHSAEPFIKEKFGYGHHELGNNLISGTCWVDSGLSKALNKNFDVSFIKGL
                     LNFELKTDGHDIFSNKINADTIDGIVRCIEYKGKNKANHLNRISIAKAAFLDNIDMTE
                     NKRLQILDNFWNTKHFVYQNFINTKHSVISDKISQIFFMENKKIAYKNLLSDERRWRE
                     QYKLLFKWLSNLKSNSIPHCLKNYEMIYTTRNYKVLKTEKDIKKRYTNNKNKAKISED
                     SLLKYREE"
     gene            complement(3076..3270)
                     /locus_tag="ACHKNP_RS24610"
                     /old_locus_tag="pgaptmp_004922"
     CDS             complement(3076..3270)
                     /locus_tag="ACHKNP_RS24610"
                     /old_locus_tag="pgaptmp_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HGU4706911.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHEL
                     AEELQNSIQVRFESESETGT"
     gene            complement(3879..>4059)
                     /locus_tag="ACHKNP_RS24615"
                     /old_locus_tag="pgaptmp_004923"
     CDS             complement(3879..>4059)
                     /locus_tag="ACHKNP_RS24615"
                     /old_locus_tag="pgaptmp_004923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706912.1"
                     /translation="HLNPQHKPIYHNISLYAPQKRAERDSDAGTAKRDVLPSENRKNA
                     SGSFSAHCIAMHENE"
BASE COUNT          981 a          835 c          833 g         1410 t
ORIGIN      
        1 aaaaaaaaag gccgcttgaa agcggccttt aagttaaaaa attaatgagg tatgtatttg
       61 attgttcttt tcaaacgcat tattaagcaa tttatataaa ttatctgtaa attctgtgct
      121 tttttcgctc agttgattgt aaagagggga gtttaatcta tctgatttat cttttatgtc
      181 gtttaaacta gctcttttct ctgggtcact gatgcagctt aagaagaact catattgaca
      241 gaagatgtct tctatcagtt caggttttgc tttgccgtgg ctgatattat atattctctc
      301 taaaggtggt atatctaaac tattagatat ttcttgtgtt cgttttgtta tgtcttgttg
      361 tgtttcactg gcatgggcga tagccattac gcctgcaaaa taaagcagtt tacgggaata
      421 gcgtagtttt atatttcgga taccccaaga cttgccatcg gcatcaactt tatgctgaaa
      481 gtcagtagtg atagtcctgt aatatcttat aacatcgtta agcaaatatt tgtcgagctt
      541 tccttcgctt gaggagttca aatatttggt aattaatttt ttcttgtaac tattgaaaat
      601 gtttttgtta tatattgctt tcccttctag tagaaacaac atccttctgg ttagagattt
      661 gtttgtatca tccccaccac caatgttttt taaaatatta tcaaatacat ctaccgcatc
      721 aacgccaaac gtacctgtat caccggcttt tttatgaatg tattttttta tgcaagcctc
      781 tatttcttct tttttatata gaatgtagtg tttttccgat tcctttttac agaaaataaa
      841 gagatctata tcagattctt ctgacgcttc tagtctaccg aaagatcccg tggtgaccat
      901 ggtgaactct aaattttttt ctgaaaatat cttttgtaga tcatcctcta gtttgtcaag
      961 aatgtcaatg ctataatttg ttgattttga aatgttttcg tattttttga ctatttcact
     1021 atagctcatt ttttttatcc ctacactctt actggtatca ttcttctctg tattttaaaa
     1081 gactgtcttc acttatcttt gctttatttt tgttgtttgt atatcttttt ttaatgtctt
     1141 tctctgtctt taacacttta taatttcttg ttgtgtatat catctcgtaa ttttttaggc
     1201 aatgaggtat tgagtttgat tttaggtttg ataaccattt aaacagtaat ttatattgct
     1261 ctctccagcg tctttcgtct gagagtaagt ttttgtatgc tatcttttta ttttccatga
     1321 aaaaaatctg acttatcttg tctgatatga cgctatgttt cgtattgata aaattttgat
     1381 atacaaaatg ctttgtattc caaaaattat ctaatatctg cagcctttta ttctctgtca
     1441 tatctatgtt atctaagaat gcagcttttg caatagaaat tctatttaaa tggtttgctt
     1501 tattctttcc tttgtactct atgcacctca ctatgccatc gatagtatcg gcatttattt
     1561 tatttgagaa gatgtcgtgt ccatcggttt ttagttcgaa gtttaacagt ccttttataa
     1621 agctaacgtc aaagtttttg tttagagcct tgcttaaacc gctgtctacc cagcatgtgc
     1681 cagatattaa attatttccg agctcatgat ggccataacc aaatttttct tttataaacg
     1741 gctcagcgct atgcgataac ggcatatgtc ctatgtcatg cagtaaagct gcggctacta
     1801 aatgctcttt gagatccgaa ttgtaattcc ttttagttgc tacatagttt gctatccctg
     1861 cgacatataa agagtgaatt gctctgtttc tttctttttt tgtgagttcg ttttgatcgc
     1921 tataatcgat agcacctaag aatgatattc cgtataagcg attaaatgcg gcagttttta
     1981 ttatcattaa taatttactg gacttttgta agtcttgaat ccagtatcca atgctttctt
     2041 tttcaatctc ctcatcaatg tcatttcttt tgaagaaatc acccataata tctgctctca
     2101 aatcattcac gttaaattga ctctaacact taatatgaca acttgctcac acaatccagt
     2161 tgacgattcg tgtcatgtca agatactgtt tacctcgttg aaaatgaaaa aaccgccttg
     2221 gcgggcggtt tttcaaaggt tctctagagt tggcgctctt ttgaaccgag gtaactggct
     2281 gatagaggag cgcagtcacc aaaacttgtt ctttcagttt agccttaacc ggcgcataac
     2341 atcaagacta actcctctaa atcagttacc aatggctgct gccaatggtg cttttgcatg
     2401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacca
     2521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcggc gcggtctttt gctctggctt
     2761 tgcctttttg ctcacatgtt ctttccggcc ttatcccctg attctgtgga taactgtgtt
     2821 accgtttttg tgtgagtcag taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca
     2881 gtgagcgagg aagcggaaaa gcgcctgaat gtgcattttc tccttacgca tctgtgcggc
     2941 atttcacacc cggcgtgggg cacttttcat acaatctgca ctgatgccgc atggttacgc
     3001 cagtatacac tccgctatcg ctacgtgact gggtcatgtc tgcgccccga cacccgccag
     3061 ggcgcgtcag cttcgttatg ttcctgtttc actttcgctc tcaaagcgaa cctggatgct
     3121 gttctggagt tcttccgcga gttcgtgcag tcgttcgcac atggccgcct gttcgtcggc
     3181 atccagagca tccagttttt caagcagtgt caggctctgg cttttgataa atcgcgccat
     3241 attcagcgca gtttgttgct gtttgttcac cttccgttgt tctccgtcag taccgggtgt
     3301 cgggtggacc ctgaccaggt ggtaattgta agacgttgcg ggcgacggta ttacaattgc
     3361 acatcctgtc ctgtttcttt aggacgtatc atggcacagc atatcaactg ataaataatc
     3421 cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg cgtcgagtgt ttcttaatca
     3481 ataattccgt tgatactccg gaaaaacgcg agtctgttct catgcctgaa atgcccacac
     3541 cccacgcaaa aacaagtttt tgctgatttt cctttataaa tagagagtta tgacaaatta
     3601 gtttctcttg ctctctttgc gatatttaaa aaagcggtgt cggcgcggtg ttgtagctgc
     3661 actgacaccg ctttgtaggg gtggtactga ctatttttat aaaaaacatc attttatatc
     3721 aggggtgctg ctggcggcgc ggtgtgtttt tttacaggac gcccctgggg gcgctgctag
     3781 gggtgtctgt tcagatatgt agcaatgagt cagggattca cgaactgaaa atgtccgaaa
     3841 atgtgtctca tgggcaccct atgcgcccaa accgttcgtc attcattttc atgcatagct
     3901 atgcagtggg ctgaaaacga tcctgacgca tttttccggt tttcactggg taaaacatct
     3961 ctttttgcag tgcctgcgtc agaatcgcgt tcagcgcgtt tctgcggtgc gtacaaactg
     4021 atgttatggt aaattggctt gtgttgagga tttaaatgc
//
LOCUS       DAZFWL020000249         4049 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFWL020000249 DAZFWL020000000
VERSION     DAZFWL020000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.250"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..249)
                     /gene="escO"
                     /locus_tag="ACHKNP_RS24620"
                     /old_locus_tag="pgaptmp_004924"
     CDS             complement(<1..249)
                     /gene="escO"
                     /locus_tag="ACHKNP_RS24620"
                     /old_locus_tag="pgaptmp_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HGU4706913.1"
                     /translation="MAKINSQIKEVDGKLDDCEQAIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTK"
     gene            complement(309..1649)
                     /gene="escN"
                     /locus_tag="ACHKNP_RS24625"
                     /old_locus_tag="pgaptmp_004925"
     CDS             complement(309..1649)
                     /gene="escN"
                     /locus_tag="ACHKNP_RS24625"
                     /old_locus_tag="pgaptmp_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HGU4706914.1"
                     /translation="MILEHDSVLERYPRIQKVLNSTVPTLSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHISGMYCGQWLSYQGEEF
                     KIRVGDELLGRLVDGIGRPMGSNITAPYLPFERSLYAEPPDPLLRQVIDQPFTLGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEFFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVKSEHLRAAAECKKLIATYKN
                     IELLIRIGEYTMGQDPEADKAIKNRKAIQSFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1633..3660)
                     /gene="escV"
                     /locus_tag="ACHKNP_RS24630"
                     /old_locus_tag="pgaptmp_004926"
     CDS             complement(1633..3660)
                     /gene="escV"
                     /locus_tag="ACHKNP_RS24630"
                     /old_locus_tag="pgaptmp_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HGU4706915.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMIVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLTDDTVYFEAGHAEISFEFVQEKLSANSIVYKTNEAN
                     QQLAHVSGMDVYAKTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENISIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVRGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERSEQIIGFLKNIVNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            complement(3657..4010)
                     /gene="cesL"
                     /locus_tag="ACHKNP_RS24635"
                     /old_locus_tag="pgaptmp_004927"
     CDS             complement(3657..4010)
                     /gene="cesL"
                     /locus_tag="ACHKNP_RS24635"
                     /old_locus_tag="pgaptmp_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HGU4706916.1"
                     /translation="MNLLIKRNVEEFLRLLGNDFYLFDNRVEIDFNGFSFFIEIIDSN
                     VFITFALEYNENAFFSFFNALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFSLMNTF
                     QQHVQIIERVRRMTS"
BASE COUNT         1259 a          927 c          726 g         1137 t
ORIGIN      
        1 ttttgttcgt ttttgtgaat cgagcaatga acgcttttct ttagaaagtg aggatttttt
       61 ctcatataat cgctgctttt gctcatcaaa tgcattgttt ttaatctggt atttatacaa
      121 cgacacttta tccagattca ccagcgatgc acaatacgcc tgcttagagg caatcgactc
      181 tttaatggct tgctcgcagt catcgagttt accatccacc tcttttatct gagagttaat
      241 cttagccatt gattctctca atcgattcgc tctgctttta cgaatagata aaattctgtc
      301 caacatattc aggcaaccac tttgaatagg ctttcaatcg ttttttcgta actactgata
      361 tctttggtgc tttgttgtat aaagctctga attgctttcc tgttttttat tgccttatcc
      421 gcttcaggat cttgccccat cgtgtactca ccaatacgaa taagcagttc aatattctta
      481 taggttgcaa taagcttttt gcattcagcc gctgcacgta aatgttccga tttaacaacg
      541 ttatgcataa cacgactggc gcttaaacca atatcaattg cagggaaatg gttttcctct
      601 gcaagttctc gggtaagcac aatatgtcca tcaagaatag agcgaacttc atcaccgatg
      661 ggatcattca cattatcgct ttctaacagc accgtataaa tcgcagttat tgaacctttt
      721 ggcgcaggtc ccgcacgctc taataatttg ggtaacgagg aaaaaacact cggagggaat
      781 ccccctctta catcaggctc tcccgatgca agcccaacat cacgcgcggc tcgggcataa
      841 cgagttacgg aatccatcat taataataca tttttgccct gatcccggaa gaactctgca
      901 atggttgtcg ccgtaaatgc ggccttcatt ctttccagcg cgggacggtc tgacgttgtg
      961 acgaccagta cacatttaga aagcgtggat tgaggtaaaa gcgcgaggaa ttcatttact
     1021 tcgcgaccac gctcgccgat aagggcaagg acaataatat ccgcggatgc gccattacaa
     1081 atcattccca aaagcgtact tttaccaacg ccggaccccg cgaaaatacc gatgcgttgc
     1141 ccaataccgc aagtaagtaa accatctatt gccctgacgc caagcgtaaa tggctgatca
     1201 ataacctgcc ttaataaagg atcggggggt tcagcataca aactgcgttc aaacggaaga
     1261 taaggtgcag taatattgct ccccattggt ctgcctatac catcaaccag tcgcccaagg
     1321 agttcgtccc caacacgaat cttgaactct tccccctgat aacttagcca ctggccacaa
     1381 tacatgccgg aaatatgctc aaaaggcagc aggaaaacct catcttcatc aatagcgatc
     1441 acctcagcta aacgttgact gggttctatc ttgtaaaagg caccaatacg cgcttttggt
     1501 aaacgcgcct tgataatcgt cccgccgata ttggtaatct tgccttcata tctggtagac
     1561 gaatttaatg ataatgtcgg cacagtgcta ttgagcactt tctggatacg tgggtatctt
     1621 tccaatacag aatcatgctc taaaatcatt taccgttcct aatactttaa gttcgatatt
     1681 attcccaact tcctgaaaag agagcacggg gactgacggg aacgaacctt caatcatttt
     1741 cttcacatag cgcctgatat ctaaagcggt cagaatgacg ccattccccg ttggattcac
     1801 aatatttttt aagaagccaa taatctgctc acttcgctcc ggtgagatat tcaaatacga
     1861 acctgatgac gtttgtctga tcgactctcg taactcattt tcaatatcag agccaataag
     1921 ccagacattc agaagtgtac cgcgaacgct atagcgacct aaaatatgcc gacggagggc
     1981 gatacgaacg tattcgcaca agataaccac atctttttct tttgtggacc aaaaaataag
     2041 cgtttcaaaa atagttcgta gatctctaat tgagatattt tcctctacga gacgctgaag
     2101 aatatccaca atcttgctca gaccaagctg acgctgaagc tctttcacaa gttcgttata
     2161 tttcctctcc atgatgtcca tcagataacg cgtttcttgt acgccgatga actctttggc
     2221 attagacaat acaagtttct ttagcaaaaa cgttatcttg tcatttgttt ttgcataaac
     2281 atccatacct gagacgtgag ctagttgttg gttagcctca ttcgttttat acacgataga
     2341 gttagccgaa agcttttcct ggacaaattc aaatgatatc tctgcatgcc cagcctcaaa
     2401 ataaacagta tcatctgtaa gagtatcttt gtatattgac tcttgataga gcaaaaattc
     2461 tatagcgcta tcattatttt taactggatt aacaataatc ttaggcaatg gaattccact
     2521 ttcctcaaag aaattccaac gcataacctc aatctgctgt gtaatatctg agctataaat
     2581 attactactt aagtttaaaa tcaacggctc ggcacccggt gagatatttt gactattaga
     2641 caaatcagct cccatagcgt ccttattatt tccggttcca aacgttcttt tcttttgtaa
     2701 tttccaggcg atacccaaca ggcaaaccgc cagaaacaag aagaccaatg taggaaagcc
     2761 aggaataatc gccattacga tcatgagtgc ggcactgatt aaaaaagggc gactattaac
     2821 agaaacctgt tgaacaatat cacctgcaag gttttcttct ttttcgcttt caccgggaac
     2881 acgagtaaca acaacccccg cagtgacgga aataataagc gcagggatct gggcaaccaa
     2941 agcatcaccg acagacaata cagaaaacag gctaagtgct tcactaaatg gcatgtcaaa
     3001 ttgccacatt ccaatgagca cgccaccaaa caggttaact aaaacaatga tgataccggc
     3061 aatagcgtcc ccttttacaa acttcatcgc cccgtccatt gaaccataaa actggctttc
     3121 cttttgcact cgggagcgaa gaacttttgc ctcaagcgga tcaataacgc ctgcgcgcat
     3181 atcaccatca atactcattt gttttcctgg cataccatca agggaaaaac gagcactcac
     3241 ttcagcaaca cgctcagccc cttttgtaat aaccataaat tgtacaatgg tgataatggt
     3301 aaaaataacc aggccaacaa caatattacc gccaacaaca aagttaccga aggagtaaat
     3361 aatgtcaccc gcatcgtgat gcagcagaat aagtctggtt gtactaacac tgagcgacag
     3421 gcgcatcaac gtcgtaatca acaggattgt aggaaaagaa gttagctcaa gaggattttt
     3481 tatatagatt gagagcatta ataaaagcaa cgctgtcgat atattaatcg cgataatgat
     3541 atccaccaca actgttggca atggaataat catcatcatt acagccataa agaagaagag
     3601 agccagaata agatcgtgat atgactctgc ttttttaaat atatttaaga gtttattcat
     3661 gatgtcatcc tgcgaactcg ttcaataatc tgcacatgct gctgaaaagt attcattaga
     3721 gaaaacacat caatattggt taacaaacaa cttaagcaca atttgttatc gtagacaaaa
     3781 atatgttcaa tcactccctg cgtccgttca ggggcaaggg cattgaaaaa agaaaaaaac
     3841 gcattttcgt tatactctaa ggcaaacgta ataaacacat tactatcaat tatttcaata
     3901 aaaaaagaaa aaccattaaa atcaatttcc acacgattat caaacaaata aaaatcattt
     3961 cctaataatc ttaaaaactc ttctacgttt cttttaatta aaagattcat atgcaggctc
     4021 tgaagtaagt atatgtgtgg atattattg
//
LOCUS       DAZFWL020000250         4047 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFWL020000250 DAZFWL020000000
VERSION     DAZFWL020000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.251"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1667
                     /locus_tag="ACHKNP_RS24640"
                     /old_locus_tag="pgaptmp_004928"
     CDS             <1..1667
                     /locus_tag="ACHKNP_RS24640"
                     /old_locus_tag="pgaptmp_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU4706917.1"
                     /translation="GVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLA
                     LAPWYGKKHRDNTLTMKRFSNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDEDIEQ
                     EGSPTFLGDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQ
                     YLKFGDKETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDG
                     ILWFSSSGEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGE
                     TWEAKIGERPDAEVMAERKEYYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESW
                     LIDRQIIMGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDIGGIDPTIVYERSK
                     KHGLFRVIPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDE
                     PLPGAVHFPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYAL
                     AALRISISRWQLDLSALLASLQEEDGAATNKKTLADYARALSGEDE"
     gene            1664..1870
                     /locus_tag="ACHKNP_RS24645"
                     /old_locus_tag="pgaptmp_004929"
     CDS             1664..1870
                     /locus_tag="ACHKNP_RS24645"
                     /old_locus_tag="pgaptmp_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HGU4706918.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            1867..3468
                     /locus_tag="ACHKNP_RS24650"
                     /old_locus_tag="pgaptmp_004930"
     CDS             1867..3468
                     /locus_tag="ACHKNP_RS24650"
                     /old_locus_tag="pgaptmp_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4706919.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSNEQRDKLAGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            3449..3910
                     /locus_tag="ACHKNP_RS24655"
                     /old_locus_tag="pgaptmp_004931"
                     /pseudo
     CDS             3449..3910
                     /locus_tag="ACHKNP_RS24655"
                     /old_locus_tag="pgaptmp_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
     gene            complement(3941..>4047)
                     /locus_tag="ACHKNP_RS24660"
                     /old_locus_tag="pgaptmp_004932"
     CDS             complement(3941..>4047)
                     /locus_tag="ACHKNP_RS24660"
                     /old_locus_tag="pgaptmp_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HGU4706920.1"
                     /translation="EPEKWLRYVIEHIQDWPANRVRDLLPWKVDLSSQ"
BASE COUNT          922 a         1047 c         1245 g          833 t
ORIGIN      
        1 tgggtgttta tgcctacttc atagagcata agcagcgcaa cacacttatc tggttgccga
       61 cggatggtga tgccgagaac tttatgaaaa cccacgttga gccgaccatc cgcgatattc
      121 cgtcgctgct ggcgctggct ccgtggtatg gcaaaaagca ccgggataac acgctcacta
      181 tgaagcgttt ttccaatggt cgtggcttct ggtgcctggg cggtaaagcg gcaaaaaact
      241 accgtgaaaa gtcggtggat gtggcgggtt atgatgaact ggctgccttt gatgaggata
      301 ttgaacagga aggctctccg acgttcctgg gcgataagcg tattgaaggc tcggtctggc
      361 caaagtccat ccgtggctcc acgcccaaag tgagaggcac ctgccagatt gagcgtgcag
      421 ccagtgaatc cccgcatttt atgcgttttc atgttgcctg cccgcactgc ggggaggagc
      481 agtatcttaa atttggcgac aaagagacgc cgtttggcct caaatggacg ccggatgacc
      541 cctccagcgt gttttatctc tgcgagcata atgcctgcgt catccgccag caggagctgg
      601 actttactga tgcccgttat atctgcgaaa agaccgggat ctggacccgt gatggcattc
      661 tctggttttc gtcatccggt gaagagattg aaccgcctga cagtgtgacc tttcacatct
      721 ggacggcgta cagcccgttc accacctggg tgcagattgt caaagactgg atgaagacga
      781 aaggggatac gggaaaacgt aaaaccttcg tgaacaccac gctcggtgag acgtgggaag
      841 cgaaaatcgg cgaacgtccg gatgctgaag tgatggcaga gcggaaagag tattattcag
      901 cgcccgttcc tgatcgtgtg gcttacctga ccgccggtat cgactcccag ctggaccgct
      961 acgaaatgcg cgtatgggga tgggggccgg gtgaggaaag ctggctgatt gaccggcaga
     1021 ttattatggg ccgccacgac gacgagcaga cgctgctgcg tgtggatgag gccatcaata
     1081 aaacctatac ccgccggaat ggtgcagaaa tgtcggtatc ccgtatctgc tgggatattg
     1141 gcgggattga cccgaccatt gtgtatgaac gctcgaaaaa gcatgggctg ttccgggtga
     1201 tccccattaa aggggcatcc gtctacggaa agccggtggc cagcatgcca cgtaagcgaa
     1261 acaaaaacgg ggtttacctt accgaaattg gtacggatac cgcgaaagag cagatttata
     1321 accgcttcac actgacgccg gaaggggatg aaccgcttcc cggtgccgtt cacttcccga
     1381 ataacccgga tatttttgat ctgaccgaag cgcagcagct gacagctgaa gagcaggtcg
     1441 aaaaatgggt ggatggcagg aaaaaaatac tgtgggacag caaaaaacga cgcaatgagg
     1501 ctctcgactg cttcgtttat gcgctggcgg cgctgcgcat cagtatttcc cgctggcagc
     1561 tggatctcag tgcgctgctg gcgagcctgc aggaagagga tggtgcagca accaacaaga
     1621 aaacactggc agattacgcc cgtgccttat ccggagagga tgaatgacgc gacaggaaga
     1681 acttgccgct gcccgtgcgg cactgcatga cctgatgaca ggtaaacggg tggcaacagt
     1741 acagaaagac ggacgaaggg tggagtttac ggccacttcc gtgtctgacc tgaaaaaata
     1801 tattgcagag ctggaagtgc agaccggcat gacacagcga cgcaggggac ctgcaggatt
     1861 ttatgtatga aaacgcccac cattcccacc cttctggggc cggacggcat gacatcgctg
     1921 cgcgaatatg ctggttatca cggcggtggc agcggatttg gtgggcagtt gcgggcgtgg
     1981 aacccaccga gtgaaagtgt ggatgcagcc ctgctgccca actttacccg tggcaatgcc
     2041 cgcgcagacg atctggtacg caataacggc tatgccgcca acgccatcca gctgcatcag
     2101 gatcatatcg tcgggtcttt tttccggctc agtcatcgcc caagctggcg ctatctgggc
     2161 atcggggagg aagaagcccg tgccttttcc cgcgaggttg aagcggcatg gaaagaattt
     2221 gccgaggatg actgctgctg cattgacgtt gagcgaaaac gcacgtttac catgatgatt
     2281 cgggaaggtg tggccatgca cgcctttaac ggagaactgt tcgttcaggc cacctgggat
     2341 accagttcgt cgcggctttt ccggacacag ttccggatgg tcagcccgaa gcgcatcagc
     2401 aatccgaaca ataccggcga cagccggaac tgccgtgccg gtgtgcagat taatgacagc
     2461 ggtgcggcgc tgggatatta cgtcagcgag gacgggtatc ctggctggat gccgcagaaa
     2521 tggacatgga taccccgtga gttacccggc gggcgcgcct cgttcattca cgtctttgaa
     2581 cccgtggagg acgggcagac ccgcggtgca aatgtgtttt acagcgtgat ggagcagatg
     2641 aagatgctcg acacgctgca gaacacgcag ctgcagagcg ccattgtgaa ggcgatgtat
     2701 gccgccacca ttgaaagtga gctggatacg cagtcagcga tggattttat tcttggcgcg
     2761 aacagtaatg agcagcggga caagctggcg ggctggattg gtgaaattgc cgcgtattac
     2821 gccgccgcgc cggtccggct gggaggcgca aaagtgccgc acctgatgcc gggtgactct
     2881 ctgaacctgc agacggctca ggacacggat aacggctact ccgtgtttga gcagtcactg
     2941 ctgcggtata tcgctgccgg actgggtgtc tcgtatgagc agctttcccg gaattacgcc
     3001 cagatgagct actccacggc acgggccagt gcgaacgagt cgtgggcgta ctttatgggg
     3061 cggcgaaaat tcgtcgcatc ccgtcaggcg agccagatgt ttctgtgctg gctggaagag
     3121 gccatcgttc gccgcgtggt gacgttacct tcaaaagcgc gtttcagctt tcaggaagcc
     3181 cgcagcgcct gggggaactg tgactggata ggctccggtc gtatggccat cgatggtctg
     3241 aaagaagtac aggaagcggt gatgctgata gaagccggac tgagtaccta cgagaaagag
     3301 tgcgcaaaac gcggtgacga ctatcaggaa atttttgccc agcaggtccg tgaaacgatg
     3361 gagcgccgcg cagccggtct taaaccgccc gcctgggcgg ctgcggcatt tgaatccgga
     3421 ctgcgacaat caacagagga ggagaagagt gacagcagag ctgcgtaatc tcccgcatat
     3481 tgccagcatg gcttttaatg agccgctgat gcttgaaccc gcctatgcgc gggttttctt
     3541 ttgtgcgctt gcaggccagc ttgggatcag ccgcctgacg gatgcggtgt ccggcgacag
     3601 cctgactgcc caggaggcac tcgcgacgct ggcattatcc ggtgatgatg acggaccacg
     3661 acaggcccgc agttatcagg tcatgaacgg catcgccgtg ctgccggtgt ccggcacgct
     3721 ggtcagccgg acgcgggcgc tgcagccgta ctcggggatg accggttaca acggcattat
     3781 cgcccgtctg caacaggctg ccagcgatcc gatggtggac ggcattctgc tcgatatgga
     3841 cacgcccggc gggatggtgg cgggggcatt tgactgcgct gacatcatcg cccgtgtgcg
     3901 tgacataaaa gtaagcgact cgtcagaacc gtattgatat ttactgagag ctcagatcaa
     3961 ctttccaggg caacagatcg cgtacccggt ttgccggcca gtcctggata tgttcaatga
     4021 cgtaacgcag ccacttttct ggctcca
//
LOCUS       DAZFWL020000251         4025 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFWL020000251 DAZFWL020000000
VERSION     DAZFWL020000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.252"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1074
                     /locus_tag="ACHKNP_RS24665"
                     /old_locus_tag="pgaptmp_004933"
     CDS             <1..1074
                     /locus_tag="ACHKNP_RS24665"
                     /old_locus_tag="pgaptmp_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HGU4706921.1"
                     /translation="KRENWREYLERERGKKPGEEKHHQRKTTLPMSVGSAGYDTQLDY
                     VVKGIIPAVSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGI
                     GVPRRVKAWEVVHGEQVKNLYLVNRPIFPAAPLDVEEMVIAARQVERETGKPVRMIIL
                     DTLARCFGGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRA
                     SLDAEYRIRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVV
                     DKPRPPVELERIEEAGNKTENHAALWQCIRTRTAHGEACTIALLRDDMKKLGYEMKHF
                     RRWLYKLENDGVIVIDGDDVRPL"
     gene            1276..1521
                     /locus_tag="ACHKNP_RS24670"
                     /old_locus_tag="pgaptmp_004934"
     CDS             1276..1521
                     /locus_tag="ACHKNP_RS24670"
                     /old_locus_tag="pgaptmp_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706922.1"
                     /translation="MNRKTRDKTAPKYKALDMTELSLKVAIRTIDRHAGEGYAKEHPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKSAGE"
     gene            1652..1846
                     /locus_tag="ACHKNP_RS24675"
                     /old_locus_tag="pgaptmp_004935"
     CDS             1652..1846
                     /locus_tag="ACHKNP_RS24675"
                     /old_locus_tag="pgaptmp_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706923.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDIVRRMNESGIYGRAATPT
                     IGHLARVLLRERLGIATSEA"
     gene            1850..2011
                     /locus_tag="ACHKNP_RS24680"
                     /old_locus_tag="pgaptmp_004936"
     CDS             1850..2011
                     /locus_tag="ACHKNP_RS24680"
                     /old_locus_tag="pgaptmp_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HGU4706924.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSDEVE
                     GLISLLGEQ"
     gene            2139..2627
                     /locus_tag="ACHKNP_RS24685"
                     /old_locus_tag="pgaptmp_004937"
     CDS             2139..2627
                     /locus_tag="ACHKNP_RS24685"
                     /old_locus_tag="pgaptmp_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4706925.1"
                     /translation="MALTTKKKRFADALSKGTNPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDPKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAANVSSNRFPIPKPPTGL
                     KH"
     gene            2639..2800
                     /locus_tag="ACHKNP_RS24690"
                     /old_locus_tag="pgaptmp_004938"
     CDS             2639..2800
                     /locus_tag="ACHKNP_RS24690"
                     /old_locus_tag="pgaptmp_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706926.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQPTEIRLQAAAVLMPYFHETLTPC
                     YQEEEDNDF"
     gene            2790..3713
                     /locus_tag="ACHKNP_RS24695"
                     /old_locus_tag="pgaptmp_004939"
     CDS             2790..3713
                     /locus_tag="ACHKNP_RS24695"
                     /old_locus_tag="pgaptmp_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4706927.1"
                     /translation="MISKNMKGVSAVRAASVLTSCKFSHADAIEMTKKNDPAVSKFLE
                     KSIVAGMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLQSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPGTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIELFKKHIANGNTLATSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGTV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
BASE COUNT         1233 a          864 c         1071 g          857 t
ORIGIN      
        1 aagcgggaga actggcgcga atatctggaa cgggagcggg ggaaaaagcc aggggaagaa
       61 aaacaccacc agcgaaaaac cacgctacca atgagcgtgg gatctgccgg atacgacaca
      121 caacttgatt acgtggttaa ggggattatt ccggcggtat cgctgtgcag catatacggg
      181 gcgagcggtt catataaatc gttccttgcg ggttcgtggg cgtgtcacgt ttccacgggc
      241 cgccagtggg gaggccgcag ggtggcgcat ggcgctgttc tctatgtggt tggtgaaggc
      301 ggtatcggcg ttccgcgtcg tgtaaaagcc tgggaggttg tgcacggtga gcaggtgaaa
      361 aatctgtatc tggtaaaccg tcccatcttt ccggctgccc cgcttgatgt tgaggaaatg
      421 gttatcgctg cccgtcaggt ggaacgggaa acgggtaaac cggttcgaat gattattctg
      481 gatacgctgg cgcgctgctt tggcggcaat gatgaaaatg attcccgtga tatgggggcg
      541 tttatccgtg ggtgtgacga actgaaacgg cgcacagggg caacggtgct ggtggttcac
      601 cattccggca aggatgaaac aaagggggcg cgtggttcca gtgcatttcg tgcatcactg
      661 gacgccgaat accggatacg cagggaggac gcaggcagcg aagcgctggt tatctcatgt
      721 accaaaatga aggacgcgga ggaactcaaa gaagcagcat atgacttacg cgtggtggag
      781 ctttttaccg acgctgacgg ggagttaatc acgtcgctgg tggtggtgga taagccgcgc
      841 cctcccgttg aactggagcg catcgaggag gcagggaaca agacggagaa tcacgccgcg
      901 ctatggcagt gcatcagaac acgaacagca cacggggagg cgtgcactat cgcccttctc
      961 cgcgacgata tgaaaaagct ggggtatgag atgaagcact tccggcgctg gctgtacaag
     1021 ctggaaaatg atggcgtaat cgttattgac ggtgatgacg tgcgcccgct gtaaaaagtg
     1081 gggagtaaaa gtggggggag tggggaattt aacaaaattg aaacataatt acccactttc
     1141 ccacctgtat atacccaaaa aagtggggag taaaaaacat attgaaaaac ataacgttag
     1201 aatcacaaaa aaaagaggtg gggagacggt gggtaatttc aaaaagtggg gagcaaaagt
     1261 ggggagtagt ggggaatgaa cagaaaaacc agagataaga cagcgccaaa atataaagcg
     1321 ttagacatga cagagctttc cttaaaggtg gcaatcagaa cgatagaccg ccacgcgggg
     1381 gaaggatacg cgaaggagca tcccgaactg ataagcgcat tcatgaccac agcggcggca
     1441 aactttgcca cgctgacaga acgggagatt gccgaagcgg aacaggtaac aaccatcaac
     1501 gttaaaagcg ctggagaata accgaagaag tgagcacata gccggagcaa tccggctttt
     1561 tttcgatctc atatatcttc aaagatggtt aattaatgat aatctatcga aaatactggt
     1621 tatgcaatga taagtatcag gagaaaacaa agtggaagag ataaagcaaa gcgctaccgt
     1681 gatgattgat catgaaatcc ttaacgagct taaaaagttg cagttcgata ttgtgcgccg
     1741 gatgaatgaa tcaggtatct acggcagggc ggcaacacca acaatagggc atcttgcgcg
     1801 cgtactgtta cgcgaacgcc tgggcattgc aacaagtgag gcgtaaacaa tggctatcac
     1861 agaacgcgag gcaagcatca ttaaagccat tggcgaggaa acaagagaca ccatagcgcc
     1921 attgatggaa aagatcgcag agcttgagac acgaataaac aagctgagtg atgaagttga
     1981 aggcctgatc tcgttactcg gtgagcaatg acaggggggg agtgttccgg ccggaaagtc
     2041 gatcgccctg gacaccgccc ccagtctcat tcatagaaaa ttttgctttt caaaaaagtt
     2101 acacgaaaag ttacaccccc tcaaaacgag gtttttgcat ggcattaacg accaaaaaaa
     2161 agcggtttgc tgatgcgtta tcaaagggca caaatccaac ggaagccgcg atacatgcgg
     2221 gatacagcga aaagactgca agggttaaag gctggcagct atccaatgat ccggaggtga
     2281 aaaagtatct taaggctgta acttctgatg taacttttca ggttacaaaa ccgaaaaaag
     2341 ttacaaagaa agttaccgaa aaagttacac cggaacgggc atcaattaac accattgaga
     2401 tgatggataa cggcctaccc gaccccatca aagcaatggg caggatactg attgacaaca
     2461 tggacacaga tccaaaactg gctttagatg ccgcgtttaa gctggcccag ttcacccacc
     2521 gcaagatcgg cagcattggg aaaaaagagg ttaaaaaact caatgccgct aatgtctcct
     2581 caaatcggtt ccccatccct aagccaccaa ctggcttaaa acactgaggt agcgaactat
     2641 gagcgaatac atcacagacc ctattgatgt actcacggaa atcatgaacg acaagcagca
     2701 gccaaccgaa atacggttac aggctgccgc tgttctgatg ccatattttc acgaaaccct
     2761 aaccccatgt taccaggagg aagaagataa tgatttctaa aaacatgaaa ggcgtgtcgg
     2821 cagttcgcgc cgccagcgtg cttacatcgt gtaaattcag ccatgcagac gcaattgaaa
     2881 tgacgaaaaa aaatgatccg gcagtatcga aatttcttga aaaatccatt gttgccggaa
     2941 tgactacgga taatatgccg tcgctggtgg cagagccgat agtaagagag cttggcgagt
     3001 atattttttc gcagtctgta ccaggcaagc taatcagcag cgcgctgaat cttccgtttg
     3061 gcgcaaaatt gcagtcaata tcaggcaagg gtagtaaatg gcttaaagaa gccgaagaaa
     3121 tccctgtcaa agagggagaa gtaaacgaaa gtgcgctaaa actgtgcaaa cttggcgcta
     3181 ttgtgatgct gaataacgag ctaatcaaat ttagcacccc tggtaccaat tcagcgatca
     3241 gagacttgat cacatcggaa tcggtgaaag aaatagataa aaaatttctt tccagtgacc
     3301 aggaggtggc gggcgtatcc cctgagggcg tgcttaatgg tgctgatgag gccgaaaact
     3361 tcatagagct tttcaaaaag cacatagcga acggtaacac gcttgccacg tcgtcgctaa
     3421 tcttgcccgt agaaaacgcc ttacagctta ctgatgctga gttctggcag atagagcttt
     3481 tgagaatacc ggtaattgtg agcgaacagg cggatcgcat gatgctgata gacgctcaaa
     3541 agctgattat taacgttgaa gccacggtta taaccccaac tgatgaggcg gtaataaaaa
     3601 ccgaatcagg gacggttagc cttttccaga atgacgctac agcgttcagg gcaatcacct
     3661 attgcggatg gcaaaaactc gataaggccg tcaccgttct ggcgaaaagc tgatttttcc
     3721 ggcatcaatc ccgatatggt gatccccata tcgggataat tacgaaattt tcgcagttta
     3781 attaatggtc agattaatcg ctaacccgct gatattttcg gaaacctcaa tttgaggaag
     3841 tcggaagcgt cacccttcaa atcctgaatg gtcataatga ccacgcagac catcaaggca
     3901 accataacga acatcgttac ggttggagct tttaccacag gaaaactcgc atttaaattg
     3961 tacacttttt cagcaaatga ttttagttat acgtttagtt atacggttta aaagttgaat
     4021 caagc
//
LOCUS       DAZFWL020000252         4023 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFWL020000252 DAZFWL020000000
VERSION     DAZFWL020000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.253"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            272..1021
                     /locus_tag="ACHKNP_RS24700"
                     /old_locus_tag="pgaptmp_004940"
     CDS             272..1021
                     /locus_tag="ACHKNP_RS24700"
                     /old_locus_tag="pgaptmp_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HGU4706928.1"
                     /translation="MSKKYQLLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLLVYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQTSGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            948..3070
                     /locus_tag="ACHKNP_RS24705"
                     /old_locus_tag="pgaptmp_004941"
                     /pseudo
     CDS             948..3070
                     /locus_tag="ACHKNP_RS24705"
                     /old_locus_tag="pgaptmp_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(3240..>4023)
                     /locus_tag="ACHKNP_RS24710"
                     /old_locus_tag="pgaptmp_004942"
     CDS             complement(3240..>4023)
                     /locus_tag="ACHKNP_RS24710"
                     /old_locus_tag="pgaptmp_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HGU4706929.1"
                     /translation="HATLKEIKDLAKNITLQYGTPDLCIEAMRKKNLKTMIFISQLKA
                     KTNFQVVGLTMKCYSPLCKNASRMDQNLRTPVPMTWAFMMREAGFPAVRSIYELPLSD
                     DVLDEVREEMRSLGSYIALNLDGSSQERTFSLSIAENLIAKIQSETDIPIVIVYGPKG
                     EDKARALVDCYNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIY
                     ADYKTRWPAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         1188 a          927 c          886 g         1022 t
ORIGIN      
        1 attctgctgc aatcaatgca tacataacac aaatatcact caggtacact actcaaacca
       61 cgctgggatt tttcctcaag ttataatcat caccccgaaa atcattcggc atttactcat
      121 taaatagtca ccccataggc ctgtacatgt ttactcagaa atatacatcc ttttctctgt
      181 cataaaccct ctgattaatc ataaataaat acttgtgaca ccaatctttt tccttaacgg
      241 aacgaattgt tgtgtagaag gagataatat tatgtcaaaa aaatatcagc tattgcttat
      301 aactcattat atgtcaacat gggtcactat aacggaagca gttgagatca ctaccaaagc
      361 cataaaacaa aaaattactc ctagcgatat ttatcgtcat gccttgagtg gcaacatcct
      421 actattggtc tatttccagt ctcctgtgat acttaagaag atacaaactt ttaatggaaa
      481 aataaaattc aggcagtttg tgggagacct acttgataaa ctatgcatgc ttgacaggga
      541 cggttttatt tatggtcaaa atttaagact ctgtacagaa gccagatata tttgtcctgt
      601 gcaacaaatt atagataccc ccctgttacg caaactcaac cagtttcgta cgtttgttcg
      661 taatgtccgc ccgggtgatg aactggatgt ccaggcacaa gttagtgaaa aaaatttaac
      721 cccgccgccg ggtaacagca gtggcaacct tgagcaacag atagccagta cttcacagct
      781 aattggatct ctgctcgccg aagatatgaa cagcgagcaa gcggcaaaca tagcacgtgg
      841 atgggcctct tctcagactt caggggtaat gacagactgg ttaagccgct tcggtaccgc
      901 aagaatcacg ctgggcgtgg atgaagattt cagcctgaag aactcccgtg acggcaaccc
      961 tcgaaaatgg cgattcgatg caacaaacag tgaactatgt gccgaacgtc acgaatgctg
     1021 aaatcacgct ggcagccttg aaggatccgg tgattgccga caataacgat ctcacgacac
     1081 taacagcacc gtcgctgata cagagggcaa tgcgatagcc aacactgagg taacatttac
     1141 tctaccggaa gatgtgaagg cgaacttcac gctgagcgat ggcgacaaag cggttactga
     1201 tgccgatggc aaagcgaaag tcacgctgaa aggtacaaaa gcaggcgctc ataccgtgac
     1261 agcatcgatg gttggcggta agagtgagca gttagtggtg aactttactg cggatacgct
     1321 cactgcgcag gttaatctta acgttaccga ggacaatttc atcgccaata acattggaat
     1381 gaccaggcta caagcaatag tgactgatgg aaacggcaac ccagtagaag gtataaaagt
     1441 taatttccgt ggaacctccg tcacgctaag cagcaccagc gttgaaacgg atgatcaggt
     1501 ttttgctgaa attcttgtga caagcaccga ggtcggacta aaaacagttt cagcctctct
     1561 ggcagataaa cctactgaag tcatctcgcg attactgaat gccaaggtag atgttaattc
     1621 tgcgacgatt accagtcagg agatacctga aggtcaggtc atggtcgcac aagacatagc
     1681 agttaaagct cacgttaacg accagtttgg caacccggtg acgcatcaag ccgcgacatt
     1741 cagtgcagcg ccatcctcgc aaatgatcat cagccagaac acggtctcta ctaatacgca
     1801 gggagtagcc gaggtcacca tgacgtctga aagaaacggc tcgtatactg taaaagcatc
     1861 cctagcgaat ggagcttcac ttgagaaaca actggaggct attgatgaaa aactgacact
     1921 cacgtccagt ccgcttatcg gtgtcaatgc tcctaaaggc gctactctga cggcaacgct
     1981 aacctctgca aatggcactc cagtggaggg tcaggtcatc aactttagcg taacgctaga
     2041 aggtgcgacg ttaagtggcg gaaaagtgag aaccaactct tcaggtcagg ctccagtcgt
     2101 tctgaccagc aataaagtcg gtacatatac ggtgactgca tcgttccata acggcgtcac
     2161 aatacagaca cagacaaccg tgaaggttac tggcaaccca agcaccgcac atgttgctag
     2221 ctttatcgct gatccgtcga ctatcgccgc caccaacagt gatttaagta ccttaaaggc
     2281 aacggttgag gatggcagtg gtaacctgat cgaaggtctc actgtgtact tcgccttaaa
     2341 aagcggctcc accacattaa cgtcattaac agcggtgacc gatcaaaacg gaatcgcgac
     2401 aacaaacgtg agatgagcga taacagggag cgtcactgta agcgcagtca taagcgcagg
     2461 tggaatgcaa acagtagata tatcgctagt ggcaggccca gcagacgcct cgcagtccat
     2521 ccttaagaac aaccagtcat cactgaaagg ggactttacc gatagtgctg agctacatct
     2581 tgttctgcac gatatatcag gcaatccgat caaagtttct gaagggatgg aatttgtgca
     2641 atcaggtaca aacgtgccct atatgaaaat tagcgcaatt gattacagtc aaaatatcaa
     2701 tggtgattac aaagctactg ttacaggaga cggagagggt atcgcaacgc tgatccccgt
     2761 attgaatggt gttcatcagg cgggcctgag taccacgata gaattcatta gcgcggaaac
     2821 cagaccaatg acgggtacag tgtcagtgaa tagtgcaaac ctaccgacag cttcattccc
     2881 ttcgcaggga tttaccgggg cgtattatca gttgaataat gacaactttg caccaggaaa
     2941 aacggcggct gattattcgt tttcaagctc ggcctcctgg gtaggcgttg atgctactgg
     3001 taaagtgaca ttcaaaaatg acggtgatag caatacagta ataatcacag cgcccccaag
     3061 atccggtggt gctatttacc aaacagttcc tcctgaatcg cgttcggtgt gactatgatc
     3121 attttcggtg agtattgctt tgatccaaag gctggttttc tgtgatgatt gaccagcctc
     3181 agagtattga tgacaccacc gttcataaca gatcggcatt actatgcata agtactgcct
     3241 caaattctcg ccaaaacact ttttaatgcc tttgcgaatt catccagact tatattgtca
     3301 attttttgcc caacgacgac tgactccgaa acatcggcca ttgcgggcca tcgcgttttg
     3361 taatcagcat aaattgcaac aacaggggta ttataggcac ttgccatatg taatattgag
     3421 gtgtcaggag ttattgcaat ataagcatct tttatgattg ctgctgaacg tttaatcgag
     3481 ggtgataagg ataaacggta gacattatta taacaatcca ctaatgccct ggctttatct
     3541 tcccctttgg gtccataaac gatcactatt ggaatatctg tttcactttg aatttttgct
     3601 attagatttt ctgcaatcga taatgaaaat gtacgttcct gcgagctacc gtctaaattg
     3661 agcgcaatgt aagatcctaa cgatcgcatt tcctcgcgta cctcatcgag tacatcatcg
     3721 cttaatggca attcatatat tgacctgact gccggaaaac ccgcctcacg catcataaat
     3781 gcccatgtca tgggtacagg agtccggaga ttctgatcca tccgggacgc gttcttacac
     3841 aaaggggaat agcacttcat ggttaaaccg acgacctgga aattcgtttt tgctttcaac
     3901 tgactgataa atatcatcgt tttcaggttc tttttacgca tcgcctctat acaaagatcc
     3961 ggcgtaccgt attgcagtgt tatattcttc gctaaatctt ttatttcttt taatgttgcg
     4021 tga
//
LOCUS       DAZFWL020000253         4006 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFWL020000253 DAZFWL020000000
VERSION     DAZFWL020000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.254"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            53..1711
                     /gene="dpiB"
                     /locus_tag="ACHKNP_RS24715"
                     /old_locus_tag="pgaptmp_004943"
     CDS             53..1711
                     /gene="dpiB"
                     /locus_tag="ACHKNP_RS24715"
                     /old_locus_tag="pgaptmp_004943"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HGU4706930.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1680..2360
                     /gene="dpiA"
                     /locus_tag="ACHKNP_RS24720"
                     /old_locus_tag="pgaptmp_004944"
                     /gene_synonym="citB"
     CDS             1680..2360
                     /gene="dpiA"
                     /locus_tag="ACHKNP_RS24720"
                     /old_locus_tag="pgaptmp_004944"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HGU4706931.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2401..3786)
                     /gene="dcuC"
                     /locus_tag="ACHKNP_RS24725"
                     /old_locus_tag="pgaptmp_004945"
     CDS             complement(2401..3786)
                     /gene="dcuC"
                     /locus_tag="ACHKNP_RS24725"
                     /old_locus_tag="pgaptmp_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU4706932.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1099 a          944 c         1034 g          929 t
ORIGIN      
        1 acattttaat tacatccgtc aaagaggctc gggacaaccc gcaaggaaaa caatgttgca
       61 gcttaacgag aataaacagt ttgcattttt ccaaagactg gcatttccgc tgcgtatctt
      121 tttgctgatt ctggtgttct caatatttgt cattgcagcc ctggcgcaat attttacggc
      181 cagttttgag gactatttaa cgcttcatgt acgcgacatg gcaatgaatc aggcgaaaat
      241 tattgcctcc aatgacagtg tcatctctgc ggtgaaaacg cgtgactaca aacggctggc
      301 gaccatcgct aacaaattac aaagagatac cgattttgat tatgtggtga ttggggaccg
      361 gcactcgatc cgcctttacc atcctaatcc ggagaaaatt ggttatccta tgcagttcac
      421 caaacagggc gcgctggaga aaggggagag ctacttcatt accgggaaag ggtcaatggg
      481 gatggcgatg cgcgccaaaa cgccaatctt tgatgacgat ggaaaagtca tcggcgtggt
      541 gtcgattggc tacctggtga gtaaaatcga tagctggcgg gctgagtttt tattaccgat
      601 ggcaggcgtg tttgtcgtgc tgttagggat tctgatgttg ctgtcgtggt tcctggcagc
      661 gcatatccgt cggcaaatga tgggcatgga gccaaagcaa atcgcacgcg tggtccgtca
      721 gcaagaggcg ctgtttagtt cggtttatga agggctgatt gcggtggatc cgcatggtta
      781 cattaccgcc atcaatcgta acgcaagaaa gatgctgggg ctgagctctc ccggacggca
      841 atggttgggt aaacccattg ctgaagtggt caggcccgcc gatttcttta ccgaacagat
      901 tgatgaaaaa cgtcaggatg tggtggctaa ctttaacggt ctgagcgtta ttgccaatcg
      961 ggaagctatt cgttcaggtg atgatttgct gggggccatt atcagctttc gtagtaaaga
     1021 cgaaatttcc accctcaatg cgcaactgac gcaaataaaa caatacgtcg agagccttcg
     1081 tacattgcga cacgagcatc tcaattggat gtcgacgctc aatggtctgt tgcagatgaa
     1141 agagtatgat cgcgtgctgg cgatggtgca gggggagtct caggcccagc aacagcttat
     1201 tgacagcctg cgtgaggcgt ttgccgatcg ccaggtggcg gggctgcttt ttggtaaagt
     1261 gcagcgcgcc cgggaactgg ggctaaaaat gatcattgtc cccggtagcc agctttcgca
     1321 actgccgcca ggactggata gcaccgagtt tgcagccatt gtgggcaatt tacttgataa
     1381 cgccttcgaa gccagcctgc gtagcgatga aggaaacaag atcgttgaat tattcctcag
     1441 cgatgaaggc gatgatgtgg tgattgaagt cgccgatcag ggctgcggcg ttccagagtc
     1501 tctacgagac aaaatatttg agcagggggt cagtacgcgt gctgacgagc ccggtgaaca
     1561 tggcattggg ttgtacttga ttgccagcta cgtaacgcgc tgcggtggtg ttatcactct
     1621 cgaagataat gatccctgcg gtaccttgtt ttcaatctat attccgaaag tgaaacctaa
     1681 tgacagctcc attaacccta ttgatcgttg aggacgaaac gccgctggca gagatgcatg
     1741 cggaatatat tcgtcacatt cccggattca gtcagatatt actggcggga aacctggcgc
     1801 aggcccgaat gatgatcgag cgttttaagc cggggctaat cttgctcgat aactatcttc
     1861 ctgacggcag agggattaat ttattgcatg aactggtgca ggcgcattat cccggcgacg
     1921 tggtgtttac cactgcagcc agcgatatgg aaacggtgtc tgaagcagta cgttgtggtg
     1981 tatttgatta tctcattaag cccattgcct atgaacggct ggggcaaacg ctaacccgtt
     2041 tccgccagcg taaacatatg ctggaaagta ttgatagcgc cagccagaag caaattgatg
     2101 agatgtttaa tgcctatgct cgcggtgaac ctaaagacga actgccgacc ggcattgatc
     2161 ccttgacgct aaacgcggtg cgaaaactgt ttaaagagcc tggtgtgcaa catacggcag
     2221 aaacggtggc gcaggcactg accatcagtc gcaccactgc caggcgttat cttgaatatt
     2281 gcgccagccg ccatctgatt attgctgaaa ttgttcacgg caaagttggc agaccgcaac
     2341 gcatatacca cagtgggtga catgataaag ccggaggaaa cttgcctccg gcattactat
     2401 ttacttgcct gtgaccgctg ctgccgttcc tggcaccatc agctctgtag caacgataac
     2461 aatcaccaaa ccaacaagca ccggtaccga ggtgcgtttt acgacttcaa acggcgagat
     2521 cttcgccatc ccggcaaccg caacgactac gccagaaacg ggcgaaaggg tacggcccag
     2581 gtttgacgcc tgcagcatcg ggatagtcaa atacgccggg ttaatgccgg aagagtgcgc
     2641 cagtttcggg atcatctcaa caaacgcata aaacggcgca ttgcctgaac cggtcgtgac
     2701 tgccgccagc atggtcagaa tcaccaatac cagcatcagg atgatactcg ccgaaccaaa
     2761 cgaggtagcg atagaaatca gactttgaat aaagccgatg gtgctaagcc cctgagcgaa
     2821 taccccagcg gcaaccagca gcatcaccac gttagcaaac gcatctgcca ttccgcgata
     2881 agccacttcc agaccagaga aaactttctg ggtattaaag ctgcggagga actccagaat
     2941 ggaggcaatc agcatacaaa tcaccagaat agtgatgatg tgtaattgcg gaccccattt
     3001 accgtcaaaa atcagtacac cgatgatcgg cgtgaacggc aaaatggcat aaaacgcagg
     3061 agcagtggtg gtgatttcac tgacatctaa catttcatga gagatgtgct cttttttatc
     3121 cagataacgt tgccagaaga agtgggcgat cgccatgccg ataattgcag caattgagat
     3181 aggcagcgtc gttttgaagg cgaagtcaat cagcgacatt tcggaagctt gcgccgccag
     3241 caccacatcc cctgaagtcg gtgcgagaat aatcgccgcc ggggaggcac aaatggcagc
     3301 agctgcgcca cgactgatac caacgtttac catcaccgga aatagggttg ccatcagcaa
     3361 aacacccaga ccggttgcgg aagagacggc cagagacatc agacaggcga caaaataggc
     3421 ggcaatcatc agcaggtaag gggagttaat atactgcaat ggttttgacg ccagcttgac
     3481 caccatatca ttcgcgccga tatgggtcat gtaagcggca aatccacaca gcatcataat
     3541 catcatgccg aggtcgccgc cgcggctcat tagtaatatt ttaacgtatt caacgatatc
     3601 cgtggcgctg tagcctgttg aagcctggct ggacggtaac actttgtgcc ccataatggc
     3661 actgataatc agcaataaca ggccaccgac aaataacaca ccagtggcgg aatacccttt
     3721 aatgatgtag cgagctacac ccacaataac cacaacccca ataaggagtt caatgaatgt
     3781 cagcataatt tttcctgtct ccaggcccca aagtaaataa taaaaaattc ctaaagctta
     3841 aggaaaaaat atgcccaata aattggcgat gaatgctgat taaaatcaag aaaaactgcc
     3901 attaagacat tgaagttgct gtttttatac atagataaca aaacgcatat ttagcggttg
     3961 gcaattatca cataataatg tgtgtaaatt gatggtggat tgatgc
//
LOCUS       DAZFWL020000254         4005 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFWL020000254 DAZFWL020000000
VERSION     DAZFWL020000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.255"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..614
                     /locus_tag="ACHKNP_RS24730"
                     /old_locus_tag="pgaptmp_004946"
     CDS             <1..614
                     /locus_tag="ACHKNP_RS24730"
                     /old_locus_tag="pgaptmp_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4706933.1"
                     /translation="LRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIVYERSKKHG
                     LFRVIPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLP
                     GAVHFPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAAL
                     RISISRWQLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            611..817
                     /locus_tag="ACHKNP_RS24735"
                     /old_locus_tag="pgaptmp_004947"
     CDS             611..817
                     /locus_tag="ACHKNP_RS24735"
                     /old_locus_tag="pgaptmp_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HGU4706934.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            814..2415
                     /locus_tag="ACHKNP_RS24740"
                     /old_locus_tag="pgaptmp_004948"
     CDS             814..2415
                     /locus_tag="ACHKNP_RS24740"
                     /old_locus_tag="pgaptmp_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4706935.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTR
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYSAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            2396..3715
                     /locus_tag="ACHKNP_RS24745"
                     /old_locus_tag="pgaptmp_004949"
     CDS             2396..3715
                     /locus_tag="ACHKNP_RS24745"
                     /old_locus_tag="pgaptmp_004949"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4706936.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAM
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            3725..>4005
                     /locus_tag="ACHKNP_RS24750"
                     /old_locus_tag="pgaptmp_004950"
     CDS             3725..>4005
                     /locus_tag="ACHKNP_RS24750"
                     /old_locus_tag="pgaptmp_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU4706937.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKASAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAAS"
BASE COUNT          895 a         1087 c         1255 g          768 t
ORIGIN      
        1 tgctgcgtgt ggatgaggcc atcaataaaa cctatacccg ccggaatggt gcagaaatgt
       61 cggtatcccg tatctgctgg gatactggcg ggattgaccc gaccattgtg tatgaacgct
      121 cgaaaaaaca tgggctgttc cgggtgatcc ccattaaagg ggcatccgtc tacggaaagc
      181 cggtggccag catgccacgt aagcgaaaca aaaacggggt ttaccttacc gaaatcggta
      241 cggataccgc gaaagagcag atttataacc gcttcacact gacgccggaa ggggatgaac
      301 cgcttcccgg tgccgttcac ttcccgaata acccggatat ttttgatctt accgaagcgc
      361 agcaactgac tgctgaagag caggtcgaaa aatgggtgga tggcaggaaa aaaatactgt
      421 gggacagcaa aaagcgacgc aatgaggcgc tcgactgctt cgtttatgcg ctggcggcgc
      481 tgcgcatcag tatttcccgc tggcagctgg atctcagtgc actgctggcg agcctgcagg
      541 aagaggatgg tgcagcaacc aacaagaaaa cactggcaga atacgcccgt gccttatccg
      601 gagaggatga atgacgcgac aggaagaact tgccgctgcc cgtgcggcac tgcatgacct
      661 gatgacagga aaacgggtgg caacggtaca gaaagacgga cggcgagtgg agtttacgac
      721 cacttccgtg tctgacctga aaaaatacat tgctgagctg gaagtgcaga ccggcatgac
      781 acagcgacgc aggggaccag caggatttta tgtatgaaaa tgtccaccat tcccaccctt
      841 ctggggccgg acggcatgac atcgctgcgt gaatatgccg gttatcacgg cggtggcagc
      901 ggatttggtg ggcagttgcg ggcgtggaac ccaccgggtg aaagtgtgga tgcagccctg
      961 ctgcccaact ttacccgtgg caatgcccgc gcagacgatc tggtacgcaa taacggctat
     1021 gccgccaacg ccatccagtt gcatcaggat catatcgtcg ggtctttttt ccgactcagt
     1081 catcgcccaa gctggcgcta tctgggcatc ggggaggaag aagcccgtgc cttttcccgc
     1141 gaggttgaag cggcatggaa agagtttgcc gaagatgact gttgctgcat tgacgttgag
     1201 cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt
     1261 gaactgttcg ttcaggccac ctgggatacc cgtccctcgc gactgttccg gacacagttc
     1321 cggatggtca gcccgaagcg catcagcaac ccgaacaata ccggcgacag ccggaactgc
     1381 cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg gatattacgt cagcgaggac
     1441 gggtatcctg gctggatgcc gcagaaatgg acatggatac cccgcgagtt acccggcggt
     1501 cgtgcttcgt tcattcacgt ctttgaaccc gtggaggacg ggcagacccg cggtgcaaat
     1561 gtgttttaca gcgtgatgga gcagatgaag atgctcgaca cgctgcagaa cacgcagctg
     1621 cagagcgcca ttgtgaaggc gatgtatgcc gccaccattg agagtgagct ggatacgcag
     1681 tcagcgatgg attttattct gggcgcgaac agtcaggagc agcgggaaag gctgaccggc
     1741 tggattggtg aaattgccgc gtattactcc gcagcaccgg tccgtctggg aggcgcaaaa
     1801 gtgccgcacc tgatgccggg ggactcactg aacctgcaga cggctcagga cacggataac
     1861 ggctactccg tgtttgagca gtcactgctg cggtatatcg ctgccgggct gggtgtctcg
     1921 tatgagcagc tttcccggaa ttacgcccag atgagctact ccacggcacg ggccagtgcg
     1981 aacgagtcgt gggcgtactt tatggggcgg cgaaaattcg tcgcatcccg tcaggcgagc
     2041 cagatgtttc tgtgctggct ggaagaggcc atcgttcgcc gcgtggtgac gttaccttca
     2101 aaagcgcgct tcagctttca ggaagcccgc agtgcctggg ggaactgcga ctggataggc
     2161 tccggtcgta tggccatcga tggtctgaaa gaagttcagg aagcggtgat gctgatagaa
     2221 gccggactga gcacctacga gaaagagtgc gcgaaacgcg gtgacgacta tcaggaaatt
     2281 tttgcccagc aggtccgtga aacgatggag cgccgcgcag ccggtcttaa accgcccgcc
     2341 tgggcggctg cggcatttga atccgggctg cgacaatcaa cagaggagga gaagagtgac
     2401 agcagagctg cgtaatctcc cgcatattgc cagcatggct tttaatgagc cgctgatgct
     2461 tgaacccgcc tatgcgcggg ttttcttttg tgcgcttgca ggccagcttg ggatcagccg
     2521 cctgacggat gcagtatccg gcgacagcct gactgccgga gaggcacccg cggcgctggc
     2581 gttatccggt gatgatgacg gaccacgaca ggcccggagt tatcaggtca tgaacggcat
     2641 cgccgtgctg ccggtgtccg gtacgctggt cagccggacg cgggcgctgc agccgtattc
     2701 ggggatgacc ggttacaacg gcattatcgc ccgtctgcaa caggctgcca gcgacccgat
     2761 ggtggacggc attctgctcg atatggacac accgggcgga atggtggcgg gagcatttga
     2821 ctgtgctgac atcatcgccc gtgtgcgtga cataaaaccg gtatgggcgc tggccaacga
     2881 catgaactgc agtgcaggtc agctgcttgc cagcgccgcc tcccggcgtc tggtcacgca
     2941 gaccgcccgg acaggctcca tcggcgtcat gatggctcac agtaattacg gtgctgccct
     3001 ggagaaacag ggcgtggaaa tcacgctgat ttacagcggc agccataagg tggatggcaa
     3061 cccctacagc catctaccgg gtgatgtccg ggaaacactg cagtcccgga tggatgcaac
     3121 ccgccggatg tttgcgcaga aggtgtcggc atataccggc ctgtccgtgc aggctgtgct
     3181 ggataccgag gctgcagtgt acagcggtca ggaggccatt gatgccggac tggctgatga
     3241 acttgtcaac agcaccgatg cgatcaccgt tatgcgtgat gcactggatg cacgtaaatc
     3301 ccgtctctca ggagggcgaa tgaccaaaga gactcaatca acaactgttt cagccactgc
     3361 ttcgcaggct gacgttactg gcgtggtgcc agcgatggag ggcgaaaacg ccagcgcggc
     3421 gcagccggac gtgaacgcgc agatcaccgc tgcggttgcg gcagaaaaca gccgcattat
     3481 ggggatcctc aactgtgagg aggctcacgg acgcgaagaa caggcccgcg tgctggcaga
     3541 aacccccggt atgaccgtgg aaacggcccg ccgcattctg gccgcagcac cacagagtgc
     3601 acaggcgcgc agtgatactg cgctggatcg tctgatgcag ggggcaccgg caccgctggc
     3661 tgcaggtaac ccggcatctg atgccgttaa cgatttgctg aacacaccag tgtaagggat
     3721 gtttatgacg agcaaagaaa cctttaccca ttaccagccg ctgggcaaca gtgacccggc
     3781 tcataccgca accgcgcccg gcggattgag tgcgaaagcg tctgcaatga ccccgctgat
     3841 gctggacacc gccacccgta agctggttgc gtgggatggc accaccgacg gtgctgccgt
     3901 tggcattctt gcggttgctg ctgaccagac cagcaccacg ctgacgttct acaagtccgg
     3961 cacgttccgt tatgaggatg tgctctggcc ggaggctgcc agcga
//
LOCUS       DAZFWL020000255         3935 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFWL020000255 DAZFWL020000000
VERSION     DAZFWL020000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.256"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(61..405)
                     /gene="yoaB"
                     /locus_tag="ACHKNP_RS24755"
                     /old_locus_tag="pgaptmp_004951"
     CDS             complement(61..405)
                     /gene="yoaB"
                     /locus_tag="ACHKNP_RS24755"
                     /old_locus_tag="pgaptmp_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU4706938.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            537..2447
                     /gene="yoaA"
                     /locus_tag="ACHKNP_RS24760"
                     /old_locus_tag="pgaptmp_004952"
     CDS             537..2447
                     /gene="yoaA"
                     /locus_tag="ACHKNP_RS24760"
                     /old_locus_tag="pgaptmp_004952"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HGU4706939.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2505..3200
                     /gene="tsaB"
                     /locus_tag="ACHKNP_RS24765"
                     /old_locus_tag="pgaptmp_004953"
     CDS             2505..3200
                     /gene="tsaB"
                     /locus_tag="ACHKNP_RS24765"
                     /old_locus_tag="pgaptmp_004953"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGU4706940.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALVYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3240..3821
                     /gene="yeaY"
                     /locus_tag="ACHKNP_RS24770"
                     /old_locus_tag="pgaptmp_004954"
     CDS             3240..3821
                     /gene="yeaY"
                     /locus_tag="ACHKNP_RS24770"
                     /old_locus_tag="pgaptmp_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HGU4706941.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
BASE COUNT          918 a         1007 c         1096 g          914 t
ORIGIN      
        1 tgtaaaaccg accagtatta tcgataaatt atcgtctgac ttattttgct tcgataaagc
       61 ttacaccgca gccacaatct taatttcaac tttatacttc gggttcatca aacccgcttg
      121 taccgtgcag cgcaccggcg catgacccgc gacaacccag gcatcccacg ctttattcat
      181 cgccgcgaag tcgtttttat cggccaggaa aatggtggca tccagaatgc tcgatttatt
      241 gctgccctgt ttttccagca cggcatcaat ctgcgccagc gtgttggcgg tttgctcaaa
      301 ggcatcggcg tcgaggtttt ccggtacacc agtgtagtag agcgtgttgt tgtggattac
      361 cacatcagac cagcgggctt cagcatcgat acgaacgata gtcatcattt tgtcctcatt
      421 atccttccat tttcgtgctg caaagactgc cacaatgtga ggtcgccgtc accaattcaa
      481 ctggcgaaag cgccctcatc ctgacataat ccctgttcaa atcaacaggg ggtagtgtga
      541 cggacgattt tgcaccagac ggtcagctgg cgaaagcgat accaggcttt aagccgcgag
      601 aaccacagcg acagatggcg gtagccgtca cccaggcgat agaaaaaggc cagccgctgg
      661 tggtggaagc aggaaccggt acgggcaaaa cctacgctta cctggctcct gcgctgcggg
      721 cgaaaaagaa agtcattatc tcgaccggct caaaagcgtt gcaggatcag ctctacagcc
      781 gcgatttgcc aacagtctca aaggcattga aatatacggg caacgtggcg ctgctgaaag
      841 ggcgctcaaa ctacctctgc ctcgagcgtc tcgaacagca ggcgctggcg gggggcgatc
      901 tgccggtaca aatcttaagc gatgtgatcc tgctgcgctc ctggtctaat caaacagtcg
      961 atggtgatat cagcacctgc gtcagcgtgg cggaagattc acaggcgtgg ccgctggtca
     1021 ccagcaccaa cgacaactgt cttggcagcg actgcccgat gtataaagat tgctttgtgg
     1081 tcaaagcacg taaaaaagcg atggacgccg atgtggtggt ggtaaaccat catctctttc
     1141 tggcggatat ggtggttaaa gagagtggat ttggcgaact gatcccggaa gcggacgtca
     1201 tgatcttcga cgaagcccac cagctaccgg acattgccag ccagtatttt ggtcagtcac
     1261 tctccagtcg acaactgctc gacctggcaa aagacatcac catcgcttac cgcaccgaat
     1321 taaaagacac ccagcagtta caaaagtgcg ctgatcgtct tgcccagagt gcgcaggatt
     1381 ttcgtctgca actcggtgag ccaggttatc gcggtaacct gcgtgagctg ttagctaatc
     1441 cgcaaattca gcgggcattt ttactgctcg atgacaccct ggaactttgt tatgacgtgg
     1501 cgaaactgtc actggggcgt tccgccttgc tggatgcggc atttgagcgc gctacgttgt
     1561 atcgcacacg gctgaagcgg ctaaaagaga tcaatcagcc gggctacagc tactggtacg
     1621 aatgcacttc gcgccatttt actctggctc tcacgccgct cagcgtggcg gataaattca
     1681 aagagttaat ggcgcaaaag cctggtagct ggatcttcac ctcagcaacg ctgtcggtga
     1741 acgacgatct gcatcatttc acctcgcggc ttgggataga acaggcggag tcgttgctat
     1801 tgccaagccc gtttgattac agccgccagg cgttactctg tgtgccgcgc aatctgccgc
     1861 aaaccaacca gccaggttct gctcgccagt tagcggcaat gctgcgaccg atcatcgaag
     1921 ctaacaacgg tcgttgtttt atgctttgta cctcgcacgc catgatgcgt gatctggccg
     1981 agcagttccg cgctaccatg acgcttcccg ttttgttgca gggggaaacc agcaaagggc
     2041 aactgttgca gcaatttgtc agcgccggta acgcgcttct tgtggcaacc agtagtttct
     2101 gggaaggggt ggacgtgcgt ggcgatacat tgtcattggt gattatcgac aagttgccgt
     2161 ttacctcgcc ggatgatcca ctgttaaaag cgcgcatgga agattgtcgt ttgcgcggtg
     2221 gcgacccgtt cgatgaagtg caactaccag atgccgtcat tactctcaaa cagggggtag
     2281 ggcgactgat tcgcgacgcc gacgatcgtg gcgtgctggt gatttgtgac aatcggctgg
     2341 tgatgcgtcc ttacggcgcg acgtttctcg ccagtctgcc gcccgcgcca cgcactcgtg
     2401 acattgcccg tgcggttcgt ttccttgcga taccatcctc caggtaattt gttacggagt
     2461 atggtatgat gcgcgccttt ttattgatct aagcacgaag acccatgcga attctggcta
     2521 tcgataccgc gacagaggcc tgctctgtcg ccctgtggaa cgacggtact gtcaacgctc
     2581 attttgagct ttgccctcgt gaacatactc aacgaatctt accgatggtg caggatatcc
     2641 tgaccaccag cggaacttcc ctgactgata ttaacgctct ggtttacggg cgcggccccg
     2701 gtagctttac cggcgtgcgc attggtattg gcattgcgca agggctggca cttggcgcag
     2761 agctaccgat gattggcgtc tccacgctaa tgacgatggc gcaaggggcg tggcgcaaaa
     2821 acggcgcaac ccgcgtgctg gcagccattg acgcgcgaat gggcgaagtt tactgggccg
     2881 aatatcagcg tgatgaaaac ggtatctggc gcggtgaaga aaccgaagcc gtactcaaac
     2941 ccgaactcgt tcatgaacga atgcaacagc tttccggcga atgggtaacg gtagggacgg
     3001 gctggcaagc ctggccggat ctcggtaaag agagcgggct ggttttgcgc gacggagaag
     3061 ttttactgcc agcggcggaa gatatgctgc cgattgcgtg tcagatgttt gccgagggta
     3121 aaacggtggc ggtggaacat gccgaaccgg tttatttacg taacaacgtc gcatggaaga
     3181 aacttccggg caaagaatga atcttaagag tatgccctga gaaaaaaagg agtcgcatca
     3241 tggcggttca aaagaatgtt atcaaaggca tactggcagg tacgtttgcg ctaatgctga
     3301 gcggttgtgt cactgtgccg gacgccatta aaggcagcag tcccacgccg caacaagatt
     3361 tagttcgggt gatgagtgcg ccgcagctgt acgttggtca ggaggcacgc tttggtggca
     3421 aagtggttgc ggtacaaaac cagcaaggga aaacccgcct ggaaattgct accgtaccgc
     3481 tggacagcgg agccagaccg acgctgggag aaccttctcg cggtcgcatt tatgccgatg
     3541 tgaacggttt tctggacccg gtggatttcc gtggacaact tgttacggta gtcgggccaa
     3601 tcactggtgc ggttgacggc aaaatcggca atacgcccta taaatttatg gtgatgcaag
     3661 taacgggtta caaacgttgg catttaaccc agcaggtgat tatgccgcct cagccgattg
     3721 atccatggtt ttatggcggt cgtggctggc cctatggcta cggcggatgg ggctggtata
     3781 atcccggccc cgcgagagta caaacagttg taactgaata attgcttgtt tttaaagaaa
     3841 aagaaacagc ggctggtccg ctgtttctgc attcttacgg taaagataaa aataaatagt
     3901 gacgcgcttc gcaacctttt cgttgggtaa ttatc
//
LOCUS       DAZFWL020000256         3895 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFWL020000256 DAZFWL020000000
VERSION     DAZFWL020000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.257"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2479)
                     /locus_tag="ACHKNP_RS24775"
                     /old_locus_tag="pgaptmp_004955"
     CDS             complement(<1..2479)
                     /locus_tag="ACHKNP_RS24775"
                     /old_locus_tag="pgaptmp_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGU4706942.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAASYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(2495..3169)
                     /locus_tag="ACHKNP_RS24780"
                     /old_locus_tag="pgaptmp_004956"
     CDS             complement(2495..3169)
                     /locus_tag="ACHKNP_RS24780"
                     /old_locus_tag="pgaptmp_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4706943.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(3250..3792)
                     /locus_tag="ACHKNP_RS24785"
                     /old_locus_tag="pgaptmp_004957"
     CDS             complement(3250..3792)
                     /locus_tag="ACHKNP_RS24785"
                     /old_locus_tag="pgaptmp_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4706944.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKEIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT         1086 a          957 c          722 g         1130 t
ORIGIN      
        1 actggcaaat atagtttcta ctttcatcaa tctctttacc gaaggtgatt gtgcatgaaa
       61 gtccttgttg cttatcaatt gctacattaa ccgatggcgg tacttcattg gtgcgaataa
      121 ataactggct tccctgaccg acaacgccaa tattatgacc atggatatca ttgacttcat
      181 aaccaaacat taatggttgc ccatccgctc ttaacgcttt tataaaccag ggcttgcgct
      241 gatcggtatc aaaattaacc agtacaaccg cgccgcgata agaggcggca attttccggt
      301 tgccacgtaa ttctgcttcg ctatcgcttt gcgacacatc cagcatcagg tgattttccc
      361 gataaggtgt cattccgtcg tataccacca ctccattacg gtttgttgtg cgatattttt
      421 gcccattgac ataagcatct ttaattcctg gcgcattcat cacagcaaac gtttcggaaa
      481 gacggttcgc cagattaacg ccccccgacc aggcgacaat gccccctgaa acactggctc
      541 cagcctgtcg ataagtactc gactgactat aactgccatt cactgtcgca accggcgcgt
      601 tccaggtcaa attcgcccca gctgtcgttt catttccctg attctgataa ctcaggttga
      661 ccccatagtt aaactgatcc cggcttccaa cggttcctga taatcccgta ttatttgagg
      721 caaacccctg atcatcaaac gtcgttgagt tagacatata tatttgccga cgaggcgtcg
      781 taacgtcatc accccaatca aagggaatcg atataaaaat attaaaacgt ttctcttcat
      841 gatgattctc gtcatacgcc tggcttgccg cgaggatata gcttatccgc cgccagttat
      901 tggaataact caactgataa tccttactgc tgccgctacg cccccagtaa tctcgccata
      961 acgtacttaa ggacacagac ccccagcctt ctggcaatga ctggctcata ttggcagaga
     1021 agctattttt acggccaaaa tcgttctggt aataatcggc aatgtcatag acatcgtttt
     1081 catcacggcg ataattatct ttattgtttg cccaaacgtg atcattaaat gtccggtaat
     1141 cacgcgatga ataacgccag gccgccagac caaaacgcgt cgacgtttgg ctcacaaatt
     1201 tgttgtaggc aatttgataa ctttgcccgt caaacacatc gccgttgtct tgtttactat
     1261 gcgacttcgt ggcatcgacg gaaatggcac caatgcgtgt attccagcca gtccccaaag
     1321 taaacgcgta ataattattc gcgaccatcg agccaccata cagcgtcaat aaattattaa
     1381 aaccatactg ataacccgcc tggacaaaat cactttgttt gctcgcccct tcaatatggc
     1441 tacgacccgc cgcaaaatca tatttcgata cgccgggttg cagcatattt ggcactgctg
     1501 cataaggcac cagataggtg gttaccgagc cgtccgcctc tttcacgctg acatcaagat
     1561 ctgctccacc accggccaac tgcaaatctg taatcgcgaa cgggccagga ggaacctctt
     1621 tctgataaac cacaaaaccg ttctgttcaa tagttaccag cgcattactc tgagcaatcc
     1681 cctgcacccg tggcgtaaaa ttttgtttcg agttaggcaa catctgcata tcacgaaaca
     1741 accgcacacc gctgaagcga acagaatcaa aaatatcgct tgatgtgtac atatcaccca
     1801 cgcgaagcgt gccgagaagt tgggcaaatc cacgttccag atacagggta ttgcttttcc
     1861 acacccctgg attgttattt gttttactga aactggcatc agaatgcagt tgccacccca
     1921 gtaaatttaa cccgctgtta aaacgtacat atgtactctt actattaccc gacgctttat
     1981 agtcactgta atactgactc acataataag aggtataaaa cgcattaata ccccgctccc
     2041 agttttccgg tggaacatag ccactttcca gttcttccac ccaggcctgc gggacactga
     2101 aatcgagacg aaaaaccccg atatcccagg tatagctccc accctgaaca agttgctcaa
     2161 atgttaaaca ttgcttaccg ctggcgaagt tatcgctatt aatgcctaac cgcttgataa
     2221 cttctattga taaacatgtt tcttgcgggt tgtctttaac aataatctca tatttcccgc
     2281 gccattgctt attgacataa atatcgatgt catactgccc cggtaagggt tgattatcat
     2341 caagacgaat atttgcaacc tgctggtctt tcattccacc tatcataaaa tgggtatcaa
     2401 aggtttcttc agctgcataa gcttcaatgc cgagcaataa cgctacgatt gctgatgcaa
     2461 gtggggtcat tctcaacata ggcagctcct gcaattaaat tttgtcacta atatagttgc
     2521 cgtggtcatc gataatggtc agataccagt tatttgcatt attacttttg acattaacac
     2581 tctgactttc taagggggca atcataatag tttcataatt gactttaaca ttattagctt
     2641 tgacatccga aatcgtcacc caattagctg agtcattttt tatcaccaat ccattgccac
     2701 tgcgatttac cagtaacttt ttaaaggtcg ctttatttac cggagcaata cccgctggcc
     2761 ggtaaaacaa cttaattctg ttttgcatcg caaacttcag tgcatttttg ccttcttgct
     2821 ctggactatt tggtggaata tccagaacat tcagataaaa aatgctttct ttattagtgg
     2881 gcagtttatt cggcataatt ttgattttta cttgctgccc ggaatttgcc cctatttttg
     2941 ccactggtgg cgttaacatg aaaggaacct gaattttttc tggtggtaac gacgtatcgc
     3001 catcatcaat ccacgcctgc agcagcgaag aacggtttcc ctggttcatc aactgcacca
     3061 taacttcttt attttctgcc gggtaaataa tgcgcgtccc gtagataaca attccggcat
     3121 gtgcaggaag gaccatagaa aaaatgagta aagataataa tcctttcatc ttaatgcctc
     3181 ttaaattaaa aagccgccat ggggatagcg gccatgttta ttacctggag aaacttacaa
     3241 ttttatttat taatcctgcg ccacacgcat aaccagagaa gataacacat cacctgattg
     3301 cactgagtta tctgtcagtt tcaggtattt tgctttaaac ggaatagcat attgaccttc
     3361 cgctgtttta taactctcaa tgttaaaagg tgcagaaaca tccaggacat cgctgttatc
     3421 acctaaatag agatagtaac ccacggtatc gttgctactg gctatctcct tatcgttacc
     3481 ggttatttta tcgttggtaa tcaacgagag ttttacacca gcaacttctt tcgggcagct
     3541 aatttttaac atgctgctgg ttacaccgac tgcatcatca taggtagaat atttagcttt
     3601 accttcaaag acagtggttg gtacagtcgg catattaata cttactgaat caccacccgc
     3661 ttcgaattta cacggagatt ctgcaatatt ccccttaata gttaatgttc cggtatcaac
     3721 gtctgcagca aatactccag cgctcataaa aaaagaagaa aagacagcgg cagcaataat
     3781 tgaacgtttc ataatatgtt ccttaataaa atttattttt ggttgttaag agtctgtatc
     3841 tcttggaaac aaatttctca catcaaaaaa gttgccgcaa tgtaaacaca ttgtt
//
LOCUS       DAZFWL020000257         3871 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFWL020000257 DAZFWL020000000
VERSION     DAZFWL020000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.258"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..126
                     /locus_tag="ACHKNP_RS24790"
                     /old_locus_tag="pgaptmp_004958"
     CDS             <1..126
                     /locus_tag="ACHKNP_RS24790"
                     /old_locus_tag="pgaptmp_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU4706945.1"
                     /translation="FWNLIKNSQECPTNTDNVLNECFNNRCTLQICPYGLKQQNP"
     gene            197..313
                     /locus_tag="ACHKNP_RS24795"
                     /old_locus_tag="pgaptmp_004959"
                     /pseudo
     CDS             197..313
                     /locus_tag="ACHKNP_RS24795"
                     /old_locus_tag="pgaptmp_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(407..538)
                     /locus_tag="ACHKNP_RS24800"
                     /old_locus_tag="pgaptmp_004960"
     CDS             complement(407..538)
                     /locus_tag="ACHKNP_RS24800"
                     /old_locus_tag="pgaptmp_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU4706946.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1039..1115)
                     /locus_tag="ACHKNP_RS24805"
                     /old_locus_tag="pgaptmp_004961"
     tRNA            complement(1039..1115)
                     /locus_tag="ACHKNP_RS24805"
                     /old_locus_tag="pgaptmp_004961"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1079..1081),aa:Arg,seq:tct)
     gene            complement(1213..1288)
                     /locus_tag="ACHKNP_RS24810"
                     /old_locus_tag="pgaptmp_004962"
     tRNA            complement(1213..1288)
                     /locus_tag="ACHKNP_RS24810"
                     /old_locus_tag="pgaptmp_004962"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1253..1255),aa:Ile,seq:cat)
     gene            complement(1338..2387)
                     /locus_tag="ACHKNP_RS24815"
                     /old_locus_tag="pgaptmp_004963"
     CDS             complement(1338..2387)
                     /locus_tag="ACHKNP_RS24815"
                     /old_locus_tag="pgaptmp_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGU4706947.1"
                     /translation="MINTVKISSCELINADCLEFIQTLPENSVDLIVTDPPYFKVKPE
                     GWDNQWEGDDDYLKWLDQCLAQFWRVLKPAGSLYLFFGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDDGYVAQGRELKQHVMA
                     PLISYFRDARKSLGITSKQIAEATGKKNMASHWFGTSQWQLPNEGNYNKLQALFARVA
                     AEKHQRGELEKPHHQLVSTYSELNRQYTELLSEYKNLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIINASSRPGDLVADFFMGSGSTVKAAMALGRCAIGVELET
                     GRFEQTVREVQDLIV"
     gene            complement(2538..2735)
                     /locus_tag="ACHKNP_RS24820"
                     /old_locus_tag="pgaptmp_004964"
     CDS             complement(2538..2735)
                     /locus_tag="ACHKNP_RS24820"
                     /old_locus_tag="pgaptmp_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4706948.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(2967..3509)
                     /locus_tag="ACHKNP_RS24825"
                     /old_locus_tag="pgaptmp_004965"
     CDS             complement(2967..3509)
                     /locus_tag="ACHKNP_RS24825"
                     /old_locus_tag="pgaptmp_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGU4706949.1"
                     /translation="MIYPEITGKSGEHLRLKTLEAVWIQGKLRMWGRWSYIGGGKPGN
                     MFNQLLASKKLTKTAINEALRRIRESGIDKPELEAFLREMIAGRQKSWLSHCTDAEAL
                     RIDGVISKALARYPGLIDILRQRYEGRGMSKRKMAELLNEVHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAQKMS"
     gene            complement(3518..>3871)
                     /locus_tag="ACHKNP_RS24830"
                     /old_locus_tag="pgaptmp_004966"
     CDS             complement(3518..>3871)
                     /locus_tag="ACHKNP_RS24830"
                     /old_locus_tag="pgaptmp_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4706950.1"
                     /translation="IEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRL
                     KLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQRV
                     PGGRLGVKIYKIESE"
BASE COUNT         1020 a         1054 c          788 g         1009 t
ORIGIN      
        1 ttctggaatt taatcaaaaa cagccaggaa tgccctacaa atacagataa cgtattgaat
       61 gaatgcttta ataaccgttg cactctgcaa atatgtcctt atgggctaaa acaacaaaat
      121 ccataaggag tttactcaca tctgacaaaa tcaatataaa cagcccctcc ggagaggggc
      181 tggagagtgg cgctatgtgc cattgcatgg tgccgggtgc ctcccggtga attcagtacc
      241 agcacctgaa tccgcgatta tcccatatac ctactcgctg attgcccctc cgcacagggg
      301 gattcaccat gccagtttct tttaacaaac tccccgcaaa ccagacaaca gtcaaccgcc
      361 tgaattgtga agtatttaaa aatttctccc gctaactgat acccggctaa cagtctggcg
      421 ttttcttttt cagcaacggg aaagcaacaa ccaccacacc cgccaccagc acaccgtcag
      481 ccagcactga cattatccgg ctgctgcaat gccattcaca aaaacagtaa gcaatcactt
      541 tttaccgtaa caggtgatta atccagatat gtatctaccc cagatgagta atccgaagtt
      601 catccatacc acaggtcctg gctattctgt tgtactcctg aacaagagca aataattctg
      661 aattagcaac catgaactca tcgcaaaccc tctgtatagc atcactattc agaataataa
      721 cgtctcttcc caaaagacga tcaggagtac agaacaaaac tgtcaaacgg ctgaaggcct
      781 ttgctcgtgc tgcattgact atatcaatac gctgcctaag gatgaaacac cccgacgcct
      841 catcaatatt cactctaccc acaccatatg aatgataaat atttaatact gaaaaaacca
      901 ttagaccgta taacaaacac tcaatcaata cttaacagaa cttttatttt tgacaaacat
      961 ataatatttt caacaatata ctgagccagg tatattccag tataaggctc tgccggaaga
     1021 aatctggaag aatgaatatg gcgcgttgta ctggattcga accagtgacc gattgcttag
     1081 aaggcaattg ctctgtccgg ctgagctaac aacgcataat gcagataatg gattgccatc
     1141 ggggacccgg gccccacaca gccagtttcg aaagctgacg ctctctgccg atgagctaat
     1201 ggcggtatgt gatggtggcc cttgctggat ttgaaccagc gacctggcga ttatgagtcg
     1261 ctcgctctca ccactgagct aaagggccgg tagcagaata ataatggtgc gtaattaatt
     1321 ctgcaatccc atccgtttca aacgattaaa tcctgaactt ccctgactgt ctgttcaaaa
     1381 cgtcctgtct ccagctcaac accaatcgca caacgcccca gtgccatcgc cgcttttacc
     1441 gttgaacctg aacccataaa aaaatctgca accaggtctc ccggacgact gctcgcgttg
     1501 attatctgct gcagcatttc tgccggtttt tcgcacggat gtttccctgg atagtactgc
     1561 accggtttat gcgtccagac atcggtgtac ggaacctgcg ccgtcacacc gaaataccgc
     1621 cgcaaatttt tatattcact cagcagttcc gtatactgcc ggttcagctc actgtatgtg
     1681 ctgaccagct ggtggtgtgg cttttccagt tccccgcgct gatgtttttc tgccgcaaca
     1741 cgcgcaaaca acgcctgcaa tttgttgtaa ttaccctcgt tcggtaactg ccactgactg
     1801 gtaccaaacc agtgcgaagc catgtttttc tttccggtgg cttccgctat ctgttttgac
     1861 gttattccca gtgatttacg cgcatcacga aagtaagaaa tcagcggggc catgacgtgc
     1921 tgttttagct cgcgcccctg tgccacataa ccatcatctt tcgggcgata cggtccctga
     1981 taatgttctg caaacagaat gcgctctgtt gccggaaaat acgcccgcag actttcctta
     2041 ttgcacccgt tccagcgtcc ggacggcttc gcccagataa tatggttcag cacattaaag
     2101 cgctcacgca tcatgatttc ggtgtcagat gccaggcgat gaccaaagaa caggtaaaga
     2161 cttccggcag gcttcagtac ccgccagaac tgcgccagac actggtccag ccatttcagg
     2221 taatcatcgt cgccctccca ctggttatcc cagccctcgg gcttcacttt aaagtatggc
     2281 gggtctgtga ctatcagatc gacagagttt tccggtaagg tctggataaa ttccaggcaa
     2341 tcagcgttga ttaactcaca actggatatt tttacagtat taatcataga tcaataagca
     2401 cttctctgat aggctcatac cgcttttgcg caaagcagat gggcctgagg tttgcttgtg
     2461 accccaacgc atgagcagat ggctggcagg tgccgctaac acccaccagc cgcccattac
     2521 cacaaattaa aaagccttca ctgcggaagg cgtctgtaac aaccgaactg ataatctgcc
     2581 agacccgcca taacaagctg agtcagtatt aactggcagc gttcgcgtga aaggtaagta
     2641 ttctgcgcaa tttccccgac ggtcgccggt tcggtgacgc ttaattcatt aaacaccact
     2701 ctggcggttt cggtcatatc ctgctgtttt agcatgcttt tttccctttt ccggttaacg
     2761 tgacatacca atacctcttg tcgaaaaagc cagcaagctg aaagaccagt attcacaact
     2821 accagcgcgt ttaatgttct gtgccgtttt tcagacataa aaaaacccgc ataaagcggg
     2881 ttctttcagg tgtccatgtc tgctattcgc ctcgcggtat agctttgcga agcgtagctg
     2941 gattgaaaca gtttatgcgt aaaaaatcaa gacatttttt gagcaaacga ttctcgcata
     3001 gggatgtata gcgcatattc agcaacagcc aaccaattag caattcgctt ttcgcatgta
     3061 ctaaaacacc attccgggtg cacctcattc aacaattcag ccattttgcg tttactcatc
     3121 ccccgccctt cgtacctttg ccgcaggata tcaatcaatc caggataacg tgcaagcgct
     3181 ttacttatca ccccatcaat gcgtaacgcc tctgcatcag tacagtgaga caaccagctc
     3241 ttctgtctgc cagcgatcat ctctcgcaag aatgcttcca gctctggttt atcaatccct
     3301 gactccctga ttctacgcag ggcttcattg attgcggttt ttgtcagttt tttggatgcc
     3361 agcaactgat tgaacatatt tcctggtttg ccaccaccta tatacgacca acgcccccac
     3421 atccgtaatt tcccctggat ccagacggct tccagcgttt ttagacgtaa atgctcgccg
     3481 cttttgcctg taatttccgg gtatatcata tttacgatca ctcactctca attttgtaaa
     3541 tcttcacgcc cagccgtccc ccaggaacgc gctgaccgcg cacaatattg atttcatcaa
     3601 actgctcgtc gtctatgaga agtccggcat gcgtcagcgc atccagtggt gctttcagga
     3661 tattgtccag gtcacgacga cgtttatccg gtggctctgc aataatcttt atcgccagcc
     3721 ttccggacag gtttaatttc agccgctgct ggcgaacaat aagcgccaca tcacggcgat
     3781 aacgctcacc ggcttttgat acaaaatatg tgctgccacg acgtcgccag taagtgttca
     3841 ccgttggcgg gtaaggcaaa acaaattcta t
//
LOCUS       DAZFWL020000258         3836 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFWL020000258 DAZFWL020000000
VERSION     DAZFWL020000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.259"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..485)
                     /locus_tag="ACHKNP_RS24835"
                     /old_locus_tag="pgaptmp_004967"
                     /pseudo
     CDS             complement(1..485)
                     /locus_tag="ACHKNP_RS24835"
                     /old_locus_tag="pgaptmp_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(719..1396)
                     /locus_tag="ACHKNP_RS24840"
                     /old_locus_tag="pgaptmp_004968"
     CDS             complement(719..1396)
                     /locus_tag="ACHKNP_RS24840"
                     /old_locus_tag="pgaptmp_004968"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HGU4706951.1"
                     /translation="MWHYLHYKVSRGKPHAECDAKRRAGSPPGPDAHQLQRSQHKKSR
                     QQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYEQRWLIEEYHKAWK
                     SGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGSISEETQNDSCEKILTPTEWKLLWV
                     KLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGWFRHQDMAEGYLVM
                     KSLDQEI"
     gene            1594..3213
                     /locus_tag="ACHKNP_RS24845"
                     /old_locus_tag="pgaptmp_004969"
     CDS             1594..3213
                     /locus_tag="ACHKNP_RS24845"
                     /old_locus_tag="pgaptmp_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc38 family transposase"
                     /protein_id="HGU4706952.1"
                     /translation="MGNSMHSLKTLLQLPCGWRCSRQIISSDGITLHLHGKRKKAQCP
                     ECFKRSDSVHSCRRRRIQHLPCSGHTLWLVFSVRHWYCRNPACSRKIFAGSLAPFAGS
                     HQQSSQALQNLQRQLGLIAGGEAGKRAATAVGLRCSADTLLRRVINTPETKQSGAPHV
                     GIDEWAWHRGHRYGTLIVNLDTHRPLVLLPGRDQRTLATWFRKYPEIQIVSRDRSGVY
                     ATAAREGAPQARQVADRWHLLKNIGDALERMMYRHIPLIRLVASELSPKKSPDPEPSV
                     PAASLRRPERLKQQTRKKRHQRWTEVMALHNKECSFREISRITGLSRVTVSRWVRSGT
                     FPEMSTRPPKRGLLDPWREWLKEQRESSNYNASRIWREMVARGFTGSETIVRDAVAKW
                     RKGWNPPVTTAVRLPSVSRVSRWLMPWRITRDEENYASRFISLMCEKEPELKIAQQLA
                     LEFYRILKTQNKSQLSSWFNRVHESGSAEFRRVAAGMEADAAAICEAISSRWSNGVVE
                     GHVNRLKMLKRQMYGRAGFELLRQRVMSPLA"
     gene            3210..>3836
                     /locus_tag="ACHKNP_RS24850"
                     /old_locus_tag="pgaptmp_004970"
     CDS             3210..>3836
                     /locus_tag="ACHKNP_RS24850"
                     /old_locus_tag="pgaptmp_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HGU4706953.1"
                     /translation="MKISTQVAEEPLSVVDTDDIFLLKQRLAEQEALIHALQEKLSNR
                     EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLHKESDTLTGRVYDPAVQR
                     PLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTTEQLELMRSAFRVIR
                     TVREKHACTQCDAIVQAPAPSRPIERGIARPGLLARVLTSKYAEHTPLY"
BASE COUNT          926 a         1003 c          960 g          947 t
ORIGIN      
        1 ttaaacgatt tatgaagaat tttactgttg tacagtacca gatactcagc cagtttcaga
       61 ttgaacatat tcagctattc aaagagcaat tcttcattaa actcaataaa ttgttccctc
      121 agtgttcggt taaaatgctc acttatggca tttattttcg gcgtatagag ataagtccat
      181 acgaacccga cggtggcttc ctgtagcgtt ttgtcgaatt ttcccgagaa ctctttgccc
      241 atcatcagtg atgatctggt tgataccaac aaggaacaac tgggctacac agcagaagaa
      301 atgcctgatg atatcacttt taagcgatag tacggccaga gagtagttgc tgtgttcgtc
      361 gagcatggta atgaaataac ggtacagctc gccattctga gctcaatagt atccatgcca
      421 ctgagcttac aggtctttat cgggcgatag tgctttattc ttcggggctt tgtggatgat
      481 ttttcgaaat gttcatgata gttaggtaaa gctaccgcat atcatttgca aaatgacaga
      541 tagatatact tcaatttgaa tcttaacaat tagtatggga atgctgtcaa tcctctgtat
      601 gcgtccacca catattgaaa tttttgttct ctaatatatt ccacgggcgt caagagtgtg
      661 aggcctgtgc tgttatacag caccagacgt tgtcccccat ctttgacctg tctcttgatc
      721 agatctcctg atcaagagac ttcatcacca ggtaaccctc agccatatcc tgatgtctga
      781 accagccatc ccacataact acccaaccgg ggctgcttgt gcgtttgctg tcatgccatc
      841 gccccagttt agccagtttc aaacaggccc atttcagggt cggtgtttgc gctggaagct
      901 gctttccttc cagcttaacc cacagtagtt tccactctgt cggcgtcagt attttctcac
      961 agctgtcatt ttgtgtttct tcactgatac ttccctgccg caggcccagc acccgcaccg
     1021 cgataaccac catgcgctca aggttatccc gggtctgcat tcgcagcgat tccacacatg
     1081 taccaccact tttccacgct ttgtggtatt cctctatcag ccagcgttgc tcgtaatggc
     1141 tgacgatacg tcgcgcatcg gcggcactcg ccactttttc tgatgtcagc ggataccagc
     1201 aggctccgtc ctctcctgct ccccgcaaca gacatacgtg agcgagagcg cctggccgct
     1261 gttgtcggga ttttttatgc tgacttcgct gtaactgatg agcatccggg cctggcgggc
     1321 tgcccgcccg cctttttgca tcacattcag cgtgtggctt cccgcggctg actttataat
     1381 gcaggtaatg ccagatatcc gcttcccggt cacagacagt gattacctgt ttctgtatct
     1441 cccccagccg ttcggccatg cgctccgaag cctgctgcca gcggtaactt tctttttctg
     1501 tcacgaacgg tgcaatagtg gttcttccgc aacttgggtg gaagaaaatg gatgaaactt
     1561 tctggtgaac aaagatctaa gctgttttaa gtcatgggca actcaatgca ctctcttaag
     1621 acacttctac agttaccttg cggatggcga tgcagtcgac aaattattag ctctgacggt
     1681 ataaccctcc atctccacgg aaaacgcaaa aaagcacaat gtcctgaatg ctttaagcgt
     1741 agcgactctg ttcatagttg tcgtcggcgc cggatacagc atctaccctg ctcaggacat
     1801 acgctatggc ttgtattttc cgtccgccac tggtactgcc gtaacccggc ttgttcacgt
     1861 aaaatttttg ccgggtcgct tgctcccttc gccggttcac accaacagtc ttcacaggcg
     1921 ttacaaaatt tacaacgtca actgggatta atcgccggag gtgaggccgg gaaacgggca
     1981 gcaacggcag tggggctccg ttgcagtgct gatactctcc ttcgcagggt tatcaatacc
     2041 ccggagacga aacagtcagg cgcgcctcat gtcggtattg atgagtgggc gtggcatcgg
     2101 ggccaccgtt acggtacgtt aatcgtcaat cttgatactc accgtcccct cgtcctgctt
     2161 cccggccgtg atcagcgtac gctggcgacc tggttcagaa aatatccgga aatacaaatt
     2221 gtctcgcgtg atcgcagtgg agtctatgcg acagcagcac gtgaaggtgc acctcaggcc
     2281 agacaggtgg ccgatcgatg gcatctgcta aaaaatattg gcgatgcgct tgaacgaatg
     2341 atgtacagac atatacctct gatacgtctt gttgccagtg agttgtcacc aaagaaatca
     2401 cctgatccag aaccatctgt gcctgcagca tcgctccgtc gtccggaacg cctaaaacaa
     2461 caaacccgca aaaaacggca tcagcgttgg acagaggtta tggccctgca taacaaggaa
     2521 tgtagtttca gggaaatatc ccgtattaca ggcctgtcgc gtgtgacagt cagtcgctgg
     2581 gtgcgttcag gaacattccc tgaaatgtca acccgacctc caaagcgagg gcttctggac
     2641 ccatggaggg agtggttaaa agagcaacga gaaagtagta attataacgc cagccggata
     2701 tggcgggaaa tggtggcccg gggatttaca ggcagtgaaa ccatcgtcag ggatgcggtt
     2761 gccaaatggc gtaaaggctg gaacccaccg gttactactg ccgtcagact tccttcagtg
     2821 tcccgggtaa gccgctggtt gatgccctgg agaataacca gggatgaaga aaattatgct
     2881 tcccgattta ttagtctgat gtgtgaaaaa gaacctgagc tgaaaatagc gcagcaactg
     2941 gcactcgagt tctaccgtat tctgaaaacc caaaataaat cacagctaag cagttggttc
     3001 aatcgagtcc acgaaagcgg ttcagcagaa tttcggcgcg tggcggcggg gatggaagct
     3061 gatgctgcag ctatatgtga ggcaatcagt agtcgctgga gtaatggtgt tgtcgaaggt
     3121 catgtaaatc gcctgaagat gctgaaacgc cagatgtatg gtcgagcagg atttgaacta
     3181 ctcaggcaga gggtaatgag tccactggca tgaaaatctc cacccaggtt gcggaagagc
     3241 cactttccgt cgttgacact gacgacatct tcctgctgaa acagcgcctg gccgaacagg
     3301 aagcgctgat ccacgccctg caggaaaagc tgagcaaccg ggagcgcgaa atagaccatc
     3361 tgcaggcgca gctggataaa ctccgccgga tgaacttcgg cagtcgttcc gaaaaagtct
     3421 cccgccgtat cgcacaaatg gaagccgatc tgaaccggct tcataaagag agcgatacgc
     3481 tgactggtag ggtgtatgac ccggcagtac agcgtccgtt gcgtcagacc cgcacccgta
     3541 agccgttccc tgaatcacta ccccgtgacg aaaagcgact gttgcctgcg gcgccgtgct
     3601 gcccgaactg cggcggttca ctgagctatc tgggcgagga taccaccgaa cagctggagt
     3661 tgatgcgtag cgcctttcgg gttatccgga cggtacggga aaaacatgcc tgtactcagt
     3721 gcgatgccat cgtgcaggca cctgcacctt cgcggcccat cgagcggggt atcgccagac
     3781 cggggctgct ggcccgtgtg ctgacctcga agtatgcaga gcacaccccg ctgtat
//
LOCUS       DAZFWL020000259         3758 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFWL020000259 DAZFWL020000000
VERSION     DAZFWL020000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.260"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..748)
                     /locus_tag="ACHKNP_RS24855"
                     /old_locus_tag="pgaptmp_004971"
     CDS             complement(<1..748)
                     /locus_tag="ACHKNP_RS24855"
                     /old_locus_tag="pgaptmp_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HGU4706954.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMT"
     gene            complement(779..1129)
                     /gene="tnpB"
                     /locus_tag="ACHKNP_RS24860"
                     /old_locus_tag="pgaptmp_004972"
     CDS             complement(779..1129)
                     /gene="tnpB"
                     /locus_tag="ACHKNP_RS24860"
                     /old_locus_tag="pgaptmp_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4706955.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(1126..1551)
                     /locus_tag="ACHKNP_RS24865"
                     /old_locus_tag="pgaptmp_004973"
     CDS             complement(1126..1551)
                     /locus_tag="ACHKNP_RS24865"
                     /old_locus_tag="pgaptmp_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4706956.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            1605..2356
                     /locus_tag="ACHKNP_RS24870"
                     /old_locus_tag="pgaptmp_004974"
                     /pseudo
     CDS             1605..2356
                     /locus_tag="ACHKNP_RS24870"
                     /old_locus_tag="pgaptmp_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          943 a          973 c          958 g          884 t
ORIGIN      
        1 gagtcattaa ctgacaacat gcgtcaaccc agttggagag taatgcccgg ctcagttcga
       61 tgccctggcg ggcaaagatt tcactctgac gatacagtgg caggtgttcg cagtattttc
      121 ccgttaacac gcgggcaagt aatccggagc ccgcgatgcc gcgctctatc gggcgggacg
      181 gcgctggcgc ttcaactata cagtcacatt ttgtacaggc tttttttacc cgaacagtgc
      241 ggatcacttt cagggcgcta ctcaccagtt ccagctgctc agcactgact tcccccagat
      301 aatccagctc actgccacac tccgggcaac aactttcttc aggctccaga cggtgtattt
      361 cacggggaag atgtgccggt aacggacgac gatgacgtga ctgtcgcaac tggcggggaa
      421 cctgcgggtc atcctcacgc ccactgtaac gatcgctttc ctgttcgcgt tgtttcagtt
      481 gggcctcagc ctgttcaacc tcacgctgca gtttttcaga acgggtaccg aacaacatcc
      541 ggcgcagttt ttctatctgg gccctcagat gttctatttc ccgctcctcc tcttcgatct
      601 tttcctcggc acgtgccagt gcagagcgca ggaaggcctc cgtctcttca accagactca
      661 gttgctgatc tttctgacgg agggcttcag cctgctcaga gagcagcctt tccagctcag
      721 tgatacgaat gaggtatttc cgactcatga ccgtttttat aatccggcca tgacattttt
      781 acaacattgt cagtgcatta aggcgggatg ttttgggctg acgccagtcc agtttatcga
      841 ggagcattgc cagctgcgag cgggtaatgg ataccttacc gtcacgcacc gcaggccaga
      901 taaactggcc ttcctccaga cgtttggtga acaggcacag accatcagca tcagcccaca
      961 gaattttaat cgtgtcaccc cgtcggccgc gaaagataaa caggtgaccg gagaaggggt
     1021 tctcatccag cacatgttgt acctgttctc ccagaccgtt gaaggattta cgcatatcag
     1081 taacgccggc aaccagccag attcgagtgc ctgatgggag cgagatcatc gtcccctccc
     1141 ggtcagttca cggatcaaca ccgtgagcag ctctggtgaa ggattttcca gcgtcatgtt
     1201 accgtggcgg aactcaactt tacaggaact ggcactgact gtgctttgtg aaggagtgga
     1261 taaaagcgga gtaagagccg ccataggctc tttctgctca tcaggcgtta tctcaacagg
     1321 taataattca acgccagcgc cagaagaggt tgttaccgga agacgccgcg atatacgcct
     1381 ttcgttctgc cagagcctga gccatttgaa caggaggtta tcattgatat cgtgttcccg
     1441 ggcaatacgg gcaacagagg ctcctggttg tgaagccagt ttaaccattt gaagtttaaa
     1501 ctcatttgaa aatgatctgc ggggttctga ggataatatt ttctgttcca taacaggtgt
     1561 ccactagttg aaaaggtggg cacctacgtt accaatactg gcttaatggc tacatacggc
     1621 ggtcagttta cgcttacgag aaagaagcgg tccgccggtc gtctgttttg actcgcagac
     1681 cggacgggcc ctcagatatc ctgaggcctg gctgcagggc tggtgtggcg gtacgctggt
     1741 gagtgacgga tacagcgtgt acaaatctct ggcggataat gaccccggta ttacatctgc
     1801 ctgttgctgg agccacgcaa ggcgcggttt cgcaaatctg tataaggcca gcagggagcc
     1861 ccgtgcagcc atggcgctga gaaaaatcgc gggtctttat cgcattgaga agttcatccg
     1921 tgaacgtcct gtggaaaaaa tacggcaatg gcgacaacgg tattcccggc cgatagtgaa
     1981 tgacctgttt gcctggcctg aagagcagga gccgtgctgt ccgcccgacg gaccactaaa
     2041 caaagcgatt aattatatcc tgaaccggcg cgatgaactg agctgcttcc tgagtgacgg
     2101 agccgtgccc ctggacaaca atatctgcga gcgtgctatc cgcccggtgg tcatggggcg
     2161 gaaagcatgg ttattcgccg gttcactgat ggccgggaac cgcgcggcac agataatgag
     2221 ccttctggaa acggcaaaac gtaatggtct ggagccgcat gcctggctga cagacgttct
     2281 gacgcgtcta ccggagtggc cggaagaccg actggaagag ttgctgcccc ttgagggatt
     2341 taccttctcc gggtgagtga tacctgccat caggtgttcg tgcagcgggc cataacccgc
     2401 agtcgggagt tgaacgcctg acgacaggaa atgagccaga gcagcgacca cgcgggctga
     2461 cagtgagtca gaacctgaat cagcccggag ccattcccgg ggctgtactt catcacctgc
     2521 catcagtacc cgtgtcagta cttcgcgacc tgacgcaatt acccgactca gttcttcaga
     2581 acccgcctcc gataccagcc tggcggtcgc cccggcgcaa cagaataaat caaccatcgt
     2641 cagactgcga tcattcgtat gcatcgctga tgatgcgccc gaaaaacgcg tcaatatgag
     2701 ttcgccggaa tattactgac gacacggttt gtagttacgt tccttcggtg agtgagtacc
     2761 tgtgcgtcag ggtaacgcaa tcagttcagg tttagcgggg tgtcaagtcg gtttggctaa
     2821 ccgaccgcag aattaactgt acctttaaag gtcgcccccg atatatctgc ctgtcagaca
     2881 tatcgccgat tcggtatctc actacataaa actccttaaa aaacaatgcg attctgttgg
     2941 tacgaagctt tcagtaagtc agtccggaga agcgccagta cgatacagaa tgaggttgaa
     3001 aaaaacgttc acgtctggtc acttcttcaa atgttaaaaa acatctcgta aagagccagt
     3061 ttatgcgggt tcgttgagta gacgtgcgag cgtcactttg tcagctttaa cgctgacgaa
     3121 agtgacgccc gcacagcttc aaactccaca tgcacacgca agcgaccaaa tttcaaatgg
     3181 tcttatgccg tgcgcacagt cataagaagc gcatgcggaa agtcagaaaa tatcttaaga
     3241 cgcagatttt aaaggcgttt ttatgccagt ctgccgggaa tacacagtag tccggttgtc
     3301 cggaagcacc acggtaatcc gaaggaaaaa tcaacgtacc tcaatcaccg tagtgatatc
     3361 cgttcccaga ggctaaacgg atgtaccacc ataaaggcat cctctttcag gctacgagga
     3421 tattgcaatc acccgaaggc gtttctctca gattgctgaa acattggctg gcagtcaccc
     3481 gaaggcgtcc ctctcagact gctggaacat tggctggtcg caaaagcgac agttaatgca
     3541 aatacaggtt aacaaaaagg gaaatctgcc gtcaacccaa ccatatgaac atgttgattt
     3601 cattatgaat gtgatgttct aatataaatt aatcagtaac ttcataaaaa gaatataaga
     3661 aacagataat gcatgtattt acacacatat cagcacaatg atagattacc accccagaaa
     3721 cacgataaag gtataattat ggaattttcc gttatttt
//
LOCUS       DAZFWL020000260         3741 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFWL020000260 DAZFWL020000000
VERSION     DAZFWL020000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.261"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(52..1590)
                     /gene="cadC"
                     /locus_tag="ACHKNP_RS24875"
                     /old_locus_tag="pgaptmp_004975"
     CDS             complement(52..1590)
                     /gene="cadC"
                     /locus_tag="ACHKNP_RS24875"
                     /old_locus_tag="pgaptmp_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HGU4706957.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQLLPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(1850..1964)
                     /locus_tag="ACHKNP_RS24880"
                     /old_locus_tag="pgaptmp_004976"
                     /pseudo
     CDS             complement(1850..1964)
                     /locus_tag="ACHKNP_RS24880"
                     /old_locus_tag="pgaptmp_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2339..2488)
                     /locus_tag="ACHKNP_RS24885"
                     /old_locus_tag="pgaptmp_004977"
     CDS             complement(2339..2488)
                     /locus_tag="ACHKNP_RS24885"
                     /old_locus_tag="pgaptmp_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4706958.1"
                     /translation="MLIAWLQDNIDCESGIIFDNDEDKTDSAALLPCIEQAREARYFP
                     SVLSA"
     gene            complement(2488..2978)
                     /locus_tag="ACHKNP_RS24890"
                     /old_locus_tag="pgaptmp_004978"
                     /pseudo
     CDS             complement(2488..2978)
                     /locus_tag="ACHKNP_RS24890"
                     /old_locus_tag="pgaptmp_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            complement(3070..3645)
                     /locus_tag="ACHKNP_RS24895"
                     /old_locus_tag="pgaptmp_004979"
                     /pseudo
     CDS             complement(3070..3645)
                     /locus_tag="ACHKNP_RS24895"
                     /old_locus_tag="pgaptmp_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
BASE COUNT         1025 a          784 c          802 g         1130 t
ORIGIN      
        1 acaaaaactc aacaacaaat attcccgagc ataaatcaac ccgggagtta cttattctga
       61 agcgagaaat ttgtcgagat aaggtacaac ataaggaaca gaagtctgga atataccatt
      121 ttcaatccag taaagggtgt ttgcccctgg gcgtaaatta aaggcggtga gatatgcatc
      181 agccgcttcc cggttcatcc ccttcatttc ataaaccttg ccaagcaaca cataatttag
      241 ccaggacatt tcaagatcaa tgccagtatt tatcgcctgg taagactcat ctgttttacc
      301 ttttaccaga gcactgaccg cttttatttg atatataatg gacaggttat tcaattccgg
      361 cagtgtaaca atgttgtcta tttctgtgtt cagtgctgct aattgttttt catctaaagg
      421 atgttgagaa tggcgcacga tatcaactaa tgctttttct gctctcgcgt aggtaaattc
      481 tggggatgat tgaacaatct cacctaataa ttcactggca cgattcaatg atttatcatc
      541 gccatgcagt aaataatcat gtgcctgata aaaattagtt aataacgcac cacgatgagg
      601 caataattgc tggagcgtct cctgcattcg ttgtggccac ggttggttta acgcttttga
      661 taaactctcc agtaaatcat tttgaatcgc cagctgatta ccgttagtga tgacataacg
      721 tttatccagc atggttgaac catctgcatt gtctaccaat tttatcgaca taaagcattg
      781 ttgagcacgg tattggcgct gattaacaaa cgcaatagat aatgttttac cggaactgct
      841 cggttcatca atgttgtagt tgattttgtc atgcaccata aaggtggaga aggtgttaag
      901 tgatgtcgcc accaaatcac ccacgcctat cgcgtaagag agctgatacg gggaactcca
      961 gctgttacaa cttttattta ccatattaat gtcaatatcg cgtggattga gcaaaatacg
     1021 cgatttgctc ataggaagac gtgtatcaag agttgaaaac gctaccagtg ctacacagat
     1081 acctaacgac aacaggaaaa aaaaccatac ccaaaaggta gtgaatcgtt tgcttttaac
     1141 tggggattgt tcaggtggcg ttgcggtgtt ttgaatgtta agactgtggg agggagaatc
     1201 tgtggcagga accgcctctg gtataggggg aggcgaagat agcattattt cctctccctc
     1261 ttcttcgctg taccagataa ccggcaccat taatttatag ccgcgctttg gtacagtagc
     1321 gatatagaca ggactatctt catcattatc ttttaatgac ttacgtagtt ctgagatact
     1381 ctgcgtcaca acatgattgg tgacaatact tctcttccag acattatcga taagttcatc
     1441 cctgctaagt acttcgccac tgtgttgagc aaagaaaacc agaagatcga ttaatctcgg
     1501 ctcaagggta agttgacgcc cattgcggct aatttggttt atggacggag taacaagcca
     1561 ttcgccaacg cgaactacag gttgttgcat aatagaaact cattcgaaaa gggaatgatg
     1621 caatgataat tgccacaacc tatttttacc atctatagat gggtttattt acatattatt
     1681 ggtgaatgca agacgttatt tttaccagcc ataaacttct tgattacata gtattacgaa
     1741 aggattttac tgagaaccag aagtaatatt ccttaccatc aaaattcatc atctttgcca
     1801 aagaaaaatg ttcagaaaat aatccatgga aaattgtccg gagcgcttac tattttaatg
     1861 gattgttagt ctttgcatga gcaagcggac tgatacattt ctctttgttc tcattcagaa
     1921 aatctcatca gtcggcgttc agaccggtgt tgtgctttat ggatgcgtta cgctcctgat
     1981 gacgtcattt gacgttcaac aacatcacgg ggccgcacga catttcacgt cagttagtgc
     2041 tatcgctcag gaacaaattt tcccgaattg ggatatgccc gcaaattgct ggtgatgtgg
     2101 ggagaatctg gttgagttcg ggagaattga tttggaggca gaacgcttaa attgtggcgt
     2161 ccagaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcggactcgg
     2221 gaatcgaaaa tttcctttat catattgata ttaatgcaat ttgtttggtt tgattaaaaa
     2281 tgcaccctca attatacccg cagtcccttt tgatatcgaa agggaatcac actcgggatc
     2341 aggctgagag cacagaagga aagtacctgg cttccctggc ctgttcaata cagggcagca
     2401 gtgctgccga atccgtttta tcctcatcgt tgtcaaagat aattcccgat tcgcagtcga
     2461 tattgtcctg cagccacgca atgaggatat acggagaagc acatccgtat catcagtgtg
     2521 acgactgcgg cggccatcca tccagtcatc ggttcgtctg agaatgacgt acaactgcgc
     2581 acgcgacacc cggagacaac ggctgactaa gcttactccc catccccggg caataagggc
     2641 gcgtgcgcta tccacttttt tgcccgtcca tattcaacgg cttctttgag gagttcattt
     2701 tccatcgttt tcttgccgag caggcgctgg agttctttaa tctgcttcat ggcggcagca
     2761 agttcagagg caggaacaac ctgttctccg gcggcgacag cagtaagact tccttcctgg
     2821 tattgcttac gccagagaaa taactggctg gctgctacac catgttgccg ggcaacgagg
     2881 gagaccgtca tccccggttc aaagctctgc tgaacaattg cgatcttttc ctgtgtggta
     2941 cgccgtctgc gtttctcagg ccctaagaca tcaatcatct gttctccaat gactagtcta
     3001 aaaactagta ttaagactat cacttattta agtgatactg gttgtctgga gattcagggg
     3061 gccagtctag ttttttgtta tgttggctga gtgacaggat gttaatctga tgtcttctac
     3121 ttcatgaaga ttatgttcta ccagacgatt gacgatatca gttggtgata aaaattcatc
     3181 tccacactta agaagaggta ggccagcaga accatcccct ggcagataaa ttttattgta
     3241 tttttctggt gttaaatcct ttggtaggat agttttcatc tctgttgccg caatacttat
     3301 agctgctgcg gcagcaacat cggtgttact tgattgtaga taacttctgt ttgcaccagg
     3361 aaaggtaaac tctgttttct ttttatcaaa atcttgtttt gtagcttcgt gcaggaataa
     3421 tatatcataa ggttttttcc ctgttgtgac tcttgaaaca ttttgaccaa gaatattaag
     3481 agcatattca cctacggatt ttgttcctaa aaacacaagc aatccttctt tctctggcac
     3541 tgtctctatt tttctccctc caggaattgt ggagatggat tgagattgtt ctgccggaat
     3601 tgatacgctg gcgcttatag gtagctgaga tcttatacta aacatacaac ctccttctta
     3661 gtttctttaa atgtgagttc atgataattc taatggagtg agtaatattt tagttgataa
     3721 tagctgatat ccttttcagc t
//
LOCUS       DAZFWL020000261         3731 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFWL020000261 DAZFWL020000000
VERSION     DAZFWL020000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.262"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            109..2389
                     /gene="ydeP"
                     /locus_tag="ACHKNP_RS24900"
                     /old_locus_tag="pgaptmp_004980"
                     /pseudo
     CDS             109..2389
                     /gene="ydeP"
                     /locus_tag="ACHKNP_RS24900"
                     /old_locus_tag="pgaptmp_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
     gene            2637..2834
                     /gene="safA"
                     /locus_tag="ACHKNP_RS24905"
                     /old_locus_tag="pgaptmp_004981"
     CDS             2637..2834
                     /gene="safA"
                     /locus_tag="ACHKNP_RS24905"
                     /old_locus_tag="pgaptmp_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HGU4706959.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            2909..3670
                     /gene="ydeO"
                     /locus_tag="ACHKNP_RS24910"
                     /old_locus_tag="pgaptmp_004982"
     CDS             2909..3670
                     /gene="ydeO"
                     /locus_tag="ACHKNP_RS24910"
                     /old_locus_tag="pgaptmp_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HGU4706960.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
BASE COUNT         1037 a          832 c          861 g         1001 t
ORIGIN      
        1 agtagtcccc tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat
       61 catcgctatt acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa
      121 attgaatcct accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca
      181 gtacgtaagc agatggatat acgccaggat gttattgcca tgtttgacat gaataagcca
      241 gagggctttg actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac
      301 atttgtgaaa acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc
      361 tctttctttg ctgagaatac ggttcaatca ttactttcct ggggagatca cgagcttgag
      421 gctgcgggac gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca
      481 ttaagctggc aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc
      541 aatcaggttg aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag
      601 ctttttgccc gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa
      661 ccgacaagcg tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa
      721 gactttgaga agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct
      781 cgcatgctga cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat
      841 cctctacagg aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg
      901 acgaactctg agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg
      961 gcgttgctca agtggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca
     1021 ggtcggccct cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag
     1081 ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt
     1141 cagacacaaa tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt
     1201 tacggaatgg ggatcactca gcacgaacat ggtacccaga acgtacagca actggtcaat
     1261 ttgctgttga tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt
     1321 cactctaatg tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt
     1381 ctggctcgcc tgggtgagcg ctatggcttc accccacctc atgcacctgg acatgctgca
     1441 attgccagca tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggg
     1501 caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa cgcaattaga
     1561 tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg cacggcatag
     1621 ctatatcctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg gtgcgcaggc
     1681 ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc
     1741 cggtgtaatg ctgaaatcag agtgtgctgt ggtcgcggga atcgcccagg caacactacc
     1801 ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc gcaatgacat
     1861 tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc ccggtggttt
     1921 tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt
     1981 cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta agctggtcat
     2041 ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg atgatcgcta
     2101 tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag ctaaaatttg
     2161 ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg gtaagcgcag
     2221 ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc gctcactggt
     2281 gacctatttt ccagaatcga atcacatgct aactcttgat aaccacgatc cattaagtgg
     2341 cattcctggt tataaaagta ttccggttga attagaacca tcaaattaat tcgcctcctc
     2401 atttcttctg ctgtcatccg cacagcagaa gaatttctca ttgactatta tcgcgcaatt
     2461 tgctcacatt gattaaatta aaccacacac tataagatat aaacctctgc ctacagctgt
     2521 aagaaactcc gctcagtact gaagtgccag tcctatttcc tcttttctcc agcctgttat
     2581 attaagcata ctgattaacg atttttaacg ttatccgcta aataaacata tttgaaatgc
     2641 atgcgaccac agtgaaaaac aaaatcacgc aaagagacaa ctataaagaa atcatgtctg
     2701 taattgtggt tgtcttatta ctgaccctta cgttgatagc cattttttcg gcaattgatc
     2761 agctgggtat ttcagaaatg ggtcgcattg caagagatct tacacatttc attatcaata
     2821 gtttgcaaga ctgaaaataa acagcaaatt ataaatatga aatgttaaaa aagtatcgat
     2881 aaaaacttta ttgttttaag gagataaaat gtcgctcgtt tgttctgtta tatttattca
     2941 tcatgccttc aacgctaaca ttttagataa agattacgcc ttctctgacg gcgagatcct
     3001 gatggtagat aacgctgttc gtacgcattt tgaaccttat gagcggcatt ttaaagagat
     3061 cggatttact gaaaatacca ttaaaaaata tctacaatgc actaacatcc agacagtgac
     3121 ggtgcctgtt cctgcgaagt ttttacgtgc ttcaaatgta ccgactggat tgcttaatga
     3181 aatgattgct tatctcaact cggaagaacg caatcatcat aatttttcag aacttttgct
     3241 tttttcttgc ctgtctattt ttgccgcatg caaaggtttc attacactat taactaacgg
     3301 tgtgctatcc gtttccggaa aagtgagaaa tattgtcaac atgaagctgg cgcacccatg
     3361 gaagctgaaa gatatttgtg actgcctgta catcagtgaa agcctgttga agaaaaaact
     3421 aaagcaagag caaacgacat tctcacagat tctattagat gccagaatgc agcacgcaaa
     3481 aaatttgata cgcgtagaag gttcagtcaa taaaattgcc gaacaatgtg gttatgccag
     3541 tacatcttat tttatttatg cgttccgcaa acatttcggc aacagtccga agagagtttc
     3601 taaggagtac cgttgtcaaa gtcacacggg tatgaatacg ggcaacacga tgagtgcttt
     3661 agctatttga ttatttgcta acgagtagtc aaccacgcac gctacgtaag aattatatgg
     3721 ggcagccatt c
//
LOCUS       DAZFWL020000262         3525 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFWL020000262 DAZFWL020000000
VERSION     DAZFWL020000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.264"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..193)
                     /locus_tag="ACHKNP_RS24915"
                     /old_locus_tag="pgaptmp_004983"
     CDS             complement(<1..193)
                     /locus_tag="ACHKNP_RS24915"
                     /old_locus_tag="pgaptmp_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HGU4706961.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLVVTS"
     gene            508..1824
                     /gene="shiA"
                     /locus_tag="ACHKNP_RS24920"
                     /old_locus_tag="pgaptmp_004984"
     CDS             508..1824
                     /gene="shiA"
                     /locus_tag="ACHKNP_RS24920"
                     /old_locus_tag="pgaptmp_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HGU4706962.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDVQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFARLADRFGRRRVYITGALIGMLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            1926..3380
                     /gene="amn"
                     /locus_tag="ACHKNP_RS24925"
                     /old_locus_tag="pgaptmp_004985"
     CDS             1926..3380
                     /gene="amn"
                     /locus_tag="ACHKNP_RS24925"
                     /old_locus_tag="pgaptmp_004985"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HGU4706963.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          838 a          848 c          834 g         1005 t
ORIGIN      
        1 tcgacgtaac aactaataga tgcttaattt tatgcaattc aagcgcccga ataacaggaa
       61 agatggccat agcatcgcca atctgatcgg gaatatgtat gacaacaaag tctgtttttt
      121 caatattaaa attataagct ttataatcgt agtaactaaa tgcaatacgt ctcaacaatg
      181 atactaaaaa cataactaac ctcgcctccc tactggttat aatgcaatgc agtctatcag
      241 aatcatcagg gtgcaatttt gtgcatatgc ggacttttat gtttcatatc tcgaacctgt
      301 gggccctctg cttaatcctt aaacaacacc agcaactcct gcgcttttat cttccatcga
      361 atttttcatg ttgccgctaa tcagccataa aatcatttgc agatgcgctc tgtcgaggta
      421 gtctcataag gttcgtttat agatcgacgg caatgtgagt taccttttcc atactaatta
      481 taaaaagaca gtacaaacag gatcattatg gactccacgc tcatctccac tcgtcccgat
      541 gaagggacgc tttcgttaag tcgcgcccga cgagctgcgt taggcagttt cgctggtgcc
      601 gtcgtcgact ggtatgattt tttactctat ggcattaccg ccgcactggt gtttaatcgc
      661 gagtttttcc cgcaagtaag cccggcgatg ggaacgctcg ccgcatttgc cacctttggt
      721 gtcggcttcc ttttccgacc gctcggcggt gtcattttcg gtcactttgg tgaccggctg
      781 ggacgtaagc gcatgttaat gctgaccgtc tggatgatgg gcatcgcgac agccttgatt
      841 ggtattcttc cttcattctc gaccattggg tggtgggcac ctattttgct ggtgacactg
      901 cgtgccattc agggatttgc cgtcggcggc gaatggggag gcgcagcgtt gctttccgtt
      961 gaaagtgcgc cgaaaaataa aaaagccttt tacagtagcg gtgtacaagt tggctacgga
     1021 gtaggtttac tgctttcaac cggactggtt tcattgatca gtatgatgac gactgacgta
     1081 cagtttttaa gctggggctg gcgcattcct ttcctgttta gcatcgtact ggttctggga
     1141 gcattgtggg tgcgcaatgg catggaggag tccgcggaat ttgaacaaca gcaacataat
     1201 caagcggccg cgaaaaaacg catcccggtt atcgaagctc tgttacgaca tcccggtgct
     1261 ttcctgaaga ttattgcgct acgactgtgc gagttgctga cgatgtacat cgttaccgcc
     1321 tttgcactta attattcaac ccagaatatg gggttaccgc gcgaactttt ccttaatatt
     1381 ggtttgctgg taggtggatt aagctgcctg acaattccct gttttgccag gcttgccgat
     1441 cgttttggtc gccgcagggt ttatatcaca ggtgcgttaa tcggaatgtt gagcgcattt
     1501 cctttcttta tggcgcttga agcacaatct attttctgga tagttttctt ctccataatg
     1561 ctggcaaaca ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt taccgaaatg
     1621 tttggtgcca gttatcgcta tagtggcgct ggagtcggtt atcaggttgc cagtgtggtt
     1681 ggtggtggat ttacaccttt tattgccgct gcactcatca cttactttgc cgggaactgg
     1741 catagcgtcg ccatttattt gctggctgga tgcctgattt ccgcaataac cgctttgttg
     1801 atgaaagaca atcaacgcgc ttgatagcct ggcgaagatc atccgatctt cgccttacac
     1861 ttttgtttca cattcctgtg acatactatc ggatgtgcgg taattgtatg gaacaggaga
     1921 cacacatgaa taataagggc tccggtctga ccccagctca ggcactggat aaactcgacg
     1981 cgctgtatga gcaatctgta gttgctttac gcaacgccat tggcaactat attacaagtg
     2041 gcgaattacc tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca tcactgaccg
     2101 taacctggga cggtagcaca accaatcccc ctaaaacgcg cgcatttggt cgttttactc
     2161 acgcaggcag ctacaccacc acgattactc gccctactct ctttcgttcg tatctcaatg
     2221 aacaacttac gttgctgtat caggattatg gtgcgcatat ctcggtgcaa ccctcgcagc
     2281 atgaaatccc ttatccttat gtcattgatg gctctgaatt gacacttgat cgctcaatga
     2341 gcgctgggtt aactcgctat ttcccgacaa cagaactggc gcaaattggc gatgaaactg
     2401 cagacggcat ttatcatcca actgaattct ccccgctatc gcattttgat gcgcgccgcg
     2461 tcgatttttc cctcgcgcgg ttgcgccatt ataccggtac gccagttgaa cattttcagc
     2521 cgttcgtctt gtttaccaac tacacacgtt atgtggatga attcgttcgt tggggatgca
     2581 gccagatcct cgatcctgat agtccctaca ttgccctttc ttgtgctggc gggaactgga
     2641 tcaccgccga aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc
     2701 agatgcccgc gtggcattta attaccgccg atggtcaggg tattactctg gtgaatattg
     2761 gcgtgggacc gtcaaatgct aaaaccatct gcgatcatct ggcagtacta cgcccggatg
     2821 tctggttgat gattggtcac tgtggtggct tacgtgaaag ccaggccatt ggcgattatg
     2881 tacttgcaca tgcttattta cgcgatgatc acgttcttga tgcggttctg ccgcccgata
     2941 ttcctattcc aagcattgct gaagtgcaac gtgcgcttta tgacgctacc aagttagtca
     3001 gcggcaggcc cggtgaggaa gtcaaacagc ggctgcgtac aggtactgtg gtaaccacag
     3061 atgacaggaa ctgggaatta cgttactcag cttctgcact tcgttttaac ttaagccggg
     3121 ccgtggcaat tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc
     3181 catacgggac actactgtgt gtttcagata aaccgttgca tggcgagatt aaacttcccg
     3241 gccaggctaa ccgtttttat gaaggcgcta tttccgaaca cctgcaaatt ggcattcggg
     3301 caatcgattt gctgcgcgca gaaggcgacc gactgcattc acgtaaatta cgaaccttta
     3361 atgagccgcc gttccgataa taagttctga accttcttat caaaaataga tgtgaaggat
     3421 taagtaagac atctggcaaa tagcccgcaa taaacgcggg ctgtttgctt ccttgggcgg
     3481 atacgagttt tattatcgtc ttaatgattt ccacatatta aaagc
//
LOCUS       DAZFWL020000263         3519 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFWL020000263 DAZFWL020000000
VERSION     DAZFWL020000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.265"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..296)
                     /locus_tag="ACHKNP_RS24930"
                     /old_locus_tag="pgaptmp_004986"
     CDS             complement(<1..296)
                     /locus_tag="ACHKNP_RS24930"
                     /old_locus_tag="pgaptmp_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4706964.1"
                     /translation="MSKVFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETES"
     gene            complement(392..580)
                     /locus_tag="ACHKNP_RS24935"
                     /old_locus_tag="pgaptmp_004987"
     CDS             complement(392..580)
                     /locus_tag="ACHKNP_RS24935"
                     /old_locus_tag="pgaptmp_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4706965.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPLKKVSLYLNNETPAG"
     gene            complement(577..765)
                     /gene="dicB"
                     /locus_tag="ACHKNP_RS24940"
                     /old_locus_tag="pgaptmp_004988"
     CDS             complement(577..765)
                     /gene="dicB"
                     /locus_tag="ACHKNP_RS24940"
                     /old_locus_tag="pgaptmp_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4706966.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            complement(1298..1672)
                     /locus_tag="ACHKNP_RS24945"
                     /old_locus_tag="pgaptmp_004989"
     CDS             complement(1298..1672)
                     /locus_tag="ACHKNP_RS24945"
                     /old_locus_tag="pgaptmp_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706967.1"
                     /translation="MEFKDLPMPFQEMAANVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(1695..1913)
                     /gene="ydfC"
                     /locus_tag="ACHKNP_RS24950"
                     /old_locus_tag="pgaptmp_004990"
     CDS             complement(1695..1913)
                     /gene="ydfC"
                     /locus_tag="ACHKNP_RS24950"
                     /old_locus_tag="pgaptmp_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGU4706968.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            complement(1943..2071)
                     /gene="ydfB"
                     /locus_tag="ACHKNP_RS24955"
                     /old_locus_tag="pgaptmp_004991"
     CDS             complement(1943..2071)
                     /gene="ydfB"
                     /locus_tag="ACHKNP_RS24955"
                     /old_locus_tag="pgaptmp_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HGU4706969.1"
                     /translation="MDFNTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSNG"
     gene            complement(2073..2228)
                     /locus_tag="ACHKNP_RS24960"
                     /old_locus_tag="pgaptmp_004992"
     CDS             complement(2073..2228)
                     /locus_tag="ACHKNP_RS24960"
                     /old_locus_tag="pgaptmp_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4706970.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTRSKTFKTENGARRW
                     LERNSGE"
     gene            complement(2501..3217)
                     /locus_tag="ACHKNP_RS24965"
                     /old_locus_tag="pgaptmp_004993"
     CDS             complement(2501..3217)
                     /locus_tag="ACHKNP_RS24965"
                     /old_locus_tag="pgaptmp_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU4706971.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKTDDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTKIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            3267..3482
                     /locus_tag="ACHKNP_RS24970"
                     /old_locus_tag="pgaptmp_004994"
     CDS             3267..3482
                     /locus_tag="ACHKNP_RS24970"
                     /old_locus_tag="pgaptmp_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU4706972.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
BASE COUNT          915 a          794 c          769 g         1041 t
ORIGIN      
        1 gattcagtct caaccgggac aaaggaagca gacgcctcat cccagcggtt ttcctgcata
       61 tattcagcat cccaggaatc gagggcaggg cggggtatgc cgggtttatc ctcacagaca
      121 ataaatttat aagcgcagtc ctgagctgca gggaattgct ccagaaattg ccaatgaaat
      181 tttgcgcgtg cgcgacgctc atcaccagct tcaatggcag tggccaccgc aacagcgcta
      241 tcttctttta tggcctgttc atcaggaata gcagcgcaaa taaagacctt actcattttg
      301 ttttaacctc attacagatt tcagggtgaa cgaatccctg ccattgctgg catttttaat
      361 ccgttggtat ggtgttaata tggctggagg attatccagc cggtgtttcg ttattcaggt
      421 acagcgatac tttttttagc gggaggcatt caccagaaat tttttgctcg tctcttgcct
      481 ggaggcagga ttctttactt gcataaattc cggtaatcac attctgtgat tcacccgtta
      541 taagaaaaac cgtcatcatc agtgcaaatg ctgaagtcat tgacgttctc cgaaaatacc
      601 aagttcaaga agagcaattc gggaaagtat ggaattatca ttgagcagat aaggctcata
      661 tttcctcata ttaatggcat cttcagtaaa ctcccggtta ctgagcagaa caccaatatc
      721 aaaacaacct tcagacgtat taacgtttgg taataacgtt tccattatcg cgtcctcaac
      781 aatgaatttt gtgatgcagt gcctggtgcc tccaggtgac gttaaccagt taacaattaa
      841 cgccggataa agagaatcca cccataacac tgtttttggt tttaactgtt ccgcgtgcgc
      901 tcagccgcat tcaccacatc acaaaattca ctttaaaaag ggcggcagag cagtcacgga
      961 gtaaaactga taccgccaaa agtcaccaga aaattgataa cagagggcgt tgcagcggag
     1021 ttgtcactta agcgtatggt caacctgaca acccggtgtc ctcaacgggg gaaggaataa
     1081 ccccgccata cttaccgccg cgccatttcg cggattgcca caaccggaag cgcacgatcg
     1141 aattaaattt aacgacgacc tatacagaga gactaacttc tccgggcgct ttcgtgttat
     1201 gccctgactt ttcagggata tatccttttc agtaaactgt cagtgccgga ttcttatccg
     1261 tgtccggcgc acgcactcta cctcacctgt gaataaatta atgattaatt gatattttgt
     1321 tgtttgattc aactttccca tcggatgtgt gatgctttaa atcacaggaa ttaatactgc
     1381 ttgctgtaaa atgattttca aggggagcta ttcgaatccc tttctttttc attaacaagc
     1441 caaatccttt attaatgatg tccattaatt ccagaaagta tttttcatgt aaatcctggt
     1501 tatcagagag ctgcttctct tcgtacagcc cgataaaggc tcggcgtacg ttaccagata
     1561 tattgtcgat ggtttctttt tctacggtac tcaggtcaag agtcgccagt tgagagcgaa
     1621 ccacattcgc tgccatttcc tggaatggca ttggtaaatc tttaaattcc attattagcc
     1681 tcgttggtta gctattaacg cgggtatgta atcattctgg caatgcttaa tgccgctgct
     1741 ttttccagcc tggtgatatc ctgctccaga gcggacagat tttcagcctg cttagccctg
     1801 gcttcattgg cccatttcag gtcctgcgca gccttaattt tctggtgcat ccactcataa
     1861 agttcatcat cggtatagtc tggcgcgatg atgacgggtt ctcgtttctg catactgatt
     1921 cctcgcggtg ctgtttcgct tatcagccgt tagattttgc cgaactggaa agcgcctgtt
     1981 taaactcact gaagctgagg gcttcttcgc cttcggcaag gccttcgaag tattcttcgt
     2041 aagccttttc catgattgtg ttgaaatcca tatcactcac ctgagtttct ttccagccag
     2101 cgacgggcac cattttcggt tttaaacgtt ttgcttctgg tatacgtcat cgcggtgaac
     2161 gtaccgtcct ggttggggaa cacgccacat accagagatt cgctgttgcc aagatcgata
     2221 gtatccatgc tgacctcatt tccccttaac gccggggtag cggaacaaaa acctgctgca
     2281 tagttattaa agttgaaccc tgccgtcatg ttcttacgcc tcgggctggc tacttaaccc
     2341 ctgaccactg ccgggtaact cgaagtattt ccctgcgttc tgtggggcgg ggtgggttgg
     2401 tatgttgtta aggtaacaag agttaccttt cgagtcaata caatgttgca aaaggtacat
     2461 ttgagggcat aaaaaacccg caatgaatgc gggttctgac tcagtctaag tattgatgta
     2521 tttgtgaaac tttaccttta atggtgtaac caccattcag ttcgatgggt ttgtaaagcg
     2581 gattcagtga caacagatag atgtttggtc cgtcaatcgc aacttttttt agtgttacgt
     2641 ttggcgttcc ttccaattgg attaagatta tttttcccac cagttctcta atgttacttg
     2701 agcatggtgt gatcagcacg gtagatccgt cggggatggt tgggaggccg ttagagtttg
     2761 tcatcgcatc tccctcaaca tgcaataaaa aagagttttc agcggttttt gtcatgacat
     2821 caacccagtt cttaatacca ggaatcttgg ttactggaca actcatatcc caataaccag
     2881 cctgttccca cgttaaaacg ggcaaccggg cgatgttgtc actaatgtaa gggtactgat
     2941 tcagacgcag atcatcggtt ttatcgtgac cgtcctttcc ataaagaatc cattcaggag
     3001 atttggaaag caattttgac agtagataca aattctcacc gtcaggtttt gaagagccat
     3061 tttcccattt tgttacggat acacgagata tgccgattgc tttcgcaacc tgctgttggg
     3121 ttaatccaac gtcttttcga cgattccgaa tacgttcgct gatagtgttt ttcatgtaac
     3181 caatgttact accaagtgat gttgctatgg ttgacattgt tatgtaacta ttgttaccct
     3241 tctgctcgaa ataacaggag agttttatgt tcaaagatga tgttctgcgc tatttcaaaa
     3301 aaaagcgact agtagctgag gctcttggaa tttcacatgt ggctgttgtg cggtggaaag
     3361 cagttattcc caaacttcgc gcaatggaac tggatgaaat tactaacggt gaattgaaat
     3421 acaacccaga actttacaag aagcaggata gcacctcgaa cgaaggaaag aatgattcat
     3481 gaaaatcaag catgaacaca tccgcatggc gatgaatgt
//
LOCUS       DAZFWL020000264         3433 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFWL020000264 DAZFWL020000000
VERSION     DAZFWL020000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.266"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(73..873)
                     /locus_tag="ACHKNP_RS24975"
                     /old_locus_tag="pgaptmp_004995"
     CDS             complement(73..873)
                     /locus_tag="ACHKNP_RS24975"
                     /old_locus_tag="pgaptmp_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706973.1"
                     /translation="MEMVVSDGEERFTELDFYYGSKALEGTSEIVTVLTNTILNREVV
                     TKIPSIEGITASFRTSYEGSFRQRFMLKITGEEQRRNYGAIGEGAFLDVMSYYLTRPL
                     LTPYNFTYKRAARIVKELESDYSSLMKRLYGPLLKLHKPIEEQNYTVELKRRRTNLAR
                     YDHTTLENLIVERADDERIIIEASITRFNRLTGTGRLILEEDSESVSFEPSYLWRDFP
                     ADQKRWLSRNLDVNTDNGDFTRVRLEVTSIRNFLGVIKKYSLHRVITD"
     gene            1672..2145
                     /locus_tag="ACHKNP_RS24980"
                     /old_locus_tag="pgaptmp_004996"
     CDS             1672..2145
                     /locus_tag="ACHKNP_RS24980"
                     /old_locus_tag="pgaptmp_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706974.1"
                     /translation="MDIDTLGGQELRDKIFAGLKIYEGKPFIERFGLFMGKAQLLEFG
                     LKKILVSFPGYNLDEEKLERLTLGQTRVELKKLGLRTDYNAYLKSFKDQRNTMAHEFL
                     ANFAVTQQLLDGAALIRTFERELDHACYAVEQLIILFDFINGAGDVTAWLEPTAP"
     gene            complement(2446..3300)
                     /locus_tag="ACHKNP_RS24985"
                     /old_locus_tag="pgaptmp_004997"
     CDS             complement(2446..3300)
                     /locus_tag="ACHKNP_RS24985"
                     /old_locus_tag="pgaptmp_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HGU4706975.1"
                     /translation="MNTNIGMRYIDVVANGNAGEFYFAYWISSNFIWPCRILDIDMGL
                     DAQVEVYDDQNHSTGMFIGVQVKTTATTLEENPNVSVPMKNIAYWESINDPVVIIRTC
                     LNNNSKEPSLYWKHLPKSELRKYLNRAEEKGYETVTIMFEEDKNSLLKTDKSAWLKIF
                     LSDYDKEIIESVVGIKSKLDKLGKYLKDNYIDEQFICGFPSDDFPSELNDTLNKYDEL
                     AVAVKVNPRLKYLSNEVKSTIDSYNSYINLILLFFKEGWSTGSIRNSDFDISSTINPA
                     LHKIINSY"
BASE COUNT         1005 a          730 c          617 g         1081 t
ORIGIN      
        1 aacggatcga aattgaagaa ataaatcaac catgtaactg atataactaa aaaggcaaca
       61 tactttatca tatcaatccg ttataactcg gtgaagacta tattttttaa tgacaccaag
      121 aaagtttctg attgatgtca cctctagccg tacacgagta aaatcaccat tatctgtgtt
      181 gacgtctaag tttctagata gccagcgttt ttgatctgct ggaaaatctc tccaaagata
      241 tgaaggttca aaacttacag actcagaatc ttcctcaaga atgagccttc ccgtccccgt
      301 tagccgatta aatcttgtaa tagaagcttc aataatgatc ctttcatcat cggcacgttc
      361 aacaattaga ttttcaaggg tcgtgtgatc ataccgagca agatttgtcc ttctccgttt
      421 cagctcaaca gtataatttt gttcttctat gggcttatga agcttcaata gtgggccata
      481 aagcctcttc attaatgaac tataatctga ctcaagttct ttaacaattc tagcagccct
      541 tttatatgtg aaattataag gtgttaataa tggtcttgta agataataag acataacatc
      601 tagaaatgcc ccctctccga ttgcgccata gtttctacgt tgttcctcac ctgtgatctt
      661 gagcatgaaa cgttgtctaa acgacccttc ataactggta cgaaaagatg cggttatgcc
      721 ttcgattgat gggattttag taactacttc gcgatttaga atcgtattag tgaggactgt
      781 aactatctct gacgtgcctt caagtgcttt tgacccatag taaaaatcaa gctctgtgaa
      841 tctttcttct ccatcactta ctaccatttc catgggaaca tgaatttcca ttctctaatt
      901 ccaaataaaa accatgggtt taatatgtcc taattgattt ggttttggca actatttttg
      961 attgtttttc aagaataatt cgaaagctat taattaatgg ctggaactaa taggttccag
     1021 ttttcaacta atccagtgac ctgctccccg ctgatcaact cagactgttg ttagcaactt
     1081 acgctcctgg tacggagcgg gcattgatta cccgctccgt tcacgttccg cctggaagtt
     1141 ctgcaattta tgtctatcgg ctattgaaaa ccttccggtc cttcccaata tcgaaactgg
     1201 cacctttgcc agataacaaa gagatacggg aacatgatgt acgtctacag aagacctcca
     1261 aatcagctcc tttccatttc tcgtttgaag cagtatttgt tgcatgaggt ggaatcaaat
     1321 caacacatca tgagacagtg ggaatctgcc agaacggcac ttcgaaacat ccgtaacaaa
     1381 gggcatgatc cagacaccgc ctcctgggtt tcacttaaaa atgatccccg agtcttcacg
     1441 aatggcgcag atgaaaagca tgggttcatt tttgttgacg acgacgatca atcagtcctt
     1501 gttgtggcct cctgggtgat agatgagtac ggttcccccc tcactacata ttaatccctg
     1561 tacctggaca gaagagtttt ctgtccacaa ctaccaatag ctttacataa tgtgtgcatc
     1621 taacttcaat aagttacttc attaccaata atgtctaaaa aggaaacgcg catggatatt
     1681 gatacgctgg gtggccagga acttcgggat aagatttttg ctgggctcaa aatatacgag
     1741 ggtaaaccct tcatcgaacg gttcggtctg tttatgggca aggcacagtt gctagagttt
     1801 ggcctaaaaa aaattcttgt ttcttttccg ggatacaacc ttgatgaaga aaaattagag
     1861 aggctgacat taggccagac ccgcgttgag cttaaaaagc tggggctgcg cacggactat
     1921 aatgcttacc tgaagtcatt caaagatcag cgcaatacga tggctcacga gttccttgct
     1981 aattttgcgg tcacacagca gcttttagac ggagctgcac ttattcgcac atttgagcgt
     2041 gagttagacc atgcctgtta cgcggtggag cagttgatca tccttttcga cttcatcaac
     2101 ggtgccggtg acgtaaccgc ctggcttgag cctactgccc cctgatactt cgtatgcttt
     2161 tatctctagg ctggacagaa tagtaattct gtccaggtat gcagaatggc tgtctatata
     2221 tcttcattag ttcacagtag gttaccggcg gctttgagcg aacagcggaa cttcgccctt
     2281 taataaatga taataaattt tcattatcac tttagttgaa aatggtgtcg aagatgaaga
     2341 gaaatgtcat attagcctac ggagtacttg tgaaataaat tcatagcatt ccctaacatc
     2401 taaacttagg gctcactgaa cgatggtgaa ccctatgcca ttgggttaat aactattgat
     2461 aattttgtga agtgcagggt taattgtcga tgaaatatca aaatcggaat ttctaatcga
     2521 cccagtgctc catccctctt tgaaaaagag cagtataagg ttaatatatg aattgtaaga
     2581 atcaatagtt gattttactt cattcgaaag atattttagt cgagggttta ctttaacagc
     2641 aactgctaat tcatcatatt tattcaaagt atcgttcagt tctgatggga aatcgtcact
     2701 tggaaaccca caaataaact gctcatcaat ataattatct tttaagtatt tacctaactt
     2761 atctagttta cttttaatcc ctactacact ttcaatgatt tccttatcat aatcgctcaa
     2821 aaatatcttt agccatgctg acttatcggt tttcagcaat gagttcttat cctcctcgaa
     2881 cattatggtt actgtttcat aacctttttc ctcggctcta tttaaatatt ttcttaattc
     2941 acttttcgga agatgcttcc agtacaagct aggctctttt gaattgttat taagacaggt
     3001 ccgaataata acaactggat catttatgct ttcccaataa gctatatttt tcatgggtac
     3061 agaaacatta gggttttctt ctagcgtagt cgcggttgtt ttcacctgca caccaataaa
     3121 cattccagtt gaatgatttt ggtcatcata aacttcaact tgagcatcta accccatatc
     3181 aatatcaaga atacggcaag gccagatgaa attacttgat atccagtatg caaaataaaa
     3241 ctctccggca ttaccatttg ccactacgtc tatatatctc ataccgatat ttgtattcat
     3301 taatactccc ccacggctaa atactatttc ccctttgtat attacaatgg tttaacgcaa
     3361 tcaactgcta aagcgctgct tttaaacttc aactaaacaa cctacaaccc attgtaatta
     3421 ctttaaaaac tat
//
LOCUS       DAZFWL020000265         3374 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFWL020000265 DAZFWL020000000
VERSION     DAZFWL020000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.267"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            24..77
                     /gene="ychE"
                     /locus_tag="ACHKNP_RS24990"
                     /old_locus_tag="pgaptmp_004998"
     CDS             24..77
                     /gene="ychE"
                     /locus_tag="ACHKNP_RS24990"
                     /old_locus_tag="pgaptmp_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HGU4706976.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            108..755
                     /gene="ychE"
                     /locus_tag="ACHKNP_RS24995"
                     /old_locus_tag="pgaptmp_004999"
     CDS             108..755
                     /gene="ychE"
                     /locus_tag="ACHKNP_RS24995"
                     /old_locus_tag="pgaptmp_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HGU4706977.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(913..1209)
                     /locus_tag="ACHKNP_RS25000"
                     /old_locus_tag="pgaptmp_005000"
     CDS             complement(913..1209)
                     /locus_tag="ACHKNP_RS25000"
                     /old_locus_tag="pgaptmp_005000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706978.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            1493..3124
                     /gene="oppA"
                     /locus_tag="ACHKNP_RS25005"
                     /old_locus_tag="pgaptmp_005001"
     CDS             1493..3124
                     /gene="oppA"
                     /locus_tag="ACHKNP_RS25005"
                     /old_locus_tag="pgaptmp_005001"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HGU4706979.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            3210..>3374
                     /gene="oppB"
                     /locus_tag="ACHKNP_RS25010"
                     /old_locus_tag="pgaptmp_005002"
     CDS             3210..>3374
                     /gene="oppB"
                     /locus_tag="ACHKNP_RS25010"
                     /old_locus_tag="pgaptmp_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter permease"
                     /protein_id="HGU4706980.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEEKYHL"
BASE COUNT          944 a          736 c          790 g          904 t
ORIGIN      
        1 tttaagtagt taggaggtga aaaatgctgt caaaaggcgt attgtcagcg cgtcttttca
       61 accttattta tggctaacat tatccggctt ttgcttcgga gctaaccgtg attcagacct
      121 tttttgattt tcccgtttac ttcaaatttt tcatcgggtt atttgcgctg gtcaacccgg
      181 tagggattat tcccgtcttt atcagcatga ccagttatca gacagcggca gcgcgaaaca
      241 aaactaacct tacagccaac ctgtctgtgg ccattatctt gtggatctcg ctttttctcg
      301 gcgacacgat tctgcaactt tttggtatat caattgattc gttccgtatc gccgggggca
      361 tcctggtggt gacgatagcg atgtcgatga tcagcggcaa gcttggcgag gataaacaga
      421 acaagcaaga aaaatcagaa accgcggtac gtgaaagcat tggtgtggtg ccattggcgt
      481 tgccgttgat ggcggggcca ggggcgatca gttctaccat cgtctggggt acgcgttatc
      541 acagcattag ctatctgttt ggtttctttg tggctattgc attgttcgct ttatgttgct
      601 ggggattgtt ccgcatggca ccgtggctgg tacgggtttt acgccagacc ggcatcaacg
      661 tgattacgcg tattatgggg ctattgctga tggcattggg gattgaattt atcgttactg
      721 gtattaaggg gattttcccc ggcctgctta attaattcct ttcaaatgaa acggagctgc
      781 catgctccgt ttacttcgtc attattttta ctttgttccc gcgcagttat caaaagcaaa
      841 aggaataggt aaaaatattc ttctcaaatt acagttagtt ataaggattt ccttaactgc
      901 ttctcctcac catcatgtta ttttcgccac atcataatcc tgggcttgct gaagaataat
      961 tgaaatgata ttagtaattc cactgccttt ggtagaggaa agtgctaaat aataatcaat
     1021 tgttaaatta ttgtgcattt cactactaga actgtaatca gaaaagatag acatgcttag
     1081 ccaatctcta tttgattgaa ttgaaagatg tttgttaagg catggatgca agctatagat
     1141 tctgatacgg tcaataaaag agaattgctt aacaattttg caaaatgtat tggcgagtaa
     1201 gaaccgcatt tggtactttc cgggcaaccg ccagacgatt atttattggt aatgagaata
     1261 attaacaatt aaagagcgtc gcgaaagaat aatgtgtctc gacaggggag acacagtacg
     1321 aatcgacata aggtgatcgt ctgaatcacc agaataaata aagtcggtga tagtaatacg
     1381 taacgataaa gtaacctgac agcagaaagt ctccgagcct gtgcagggtc ccaatccggg
     1441 attacacatg ctggttaata ccagtaatta taatgaggga gtccaaaaaa caatgaccaa
     1501 catcaccaag agaagtttag tagcagctgg cgttctggct gcgctaatgg cagggaatgt
     1561 cgcgctggca gctgatgtac ccgcaggcgt cacactggcg gaaaaacaaa cactggtacg
     1621 taacaatggt tcagaagttc agtcattaga tccgcacaaa attgaaggtg ttccggagtc
     1681 taatatcagc cgagacctgt ttgaaggctt actggtcagc gatcttgacg gtcatccagc
     1741 acctggcgtc gctgaatcct gggataataa agacgcgaaa gtctggacct tccatttgcg
     1801 taaagatgcg aaatggtctg atggcacgcc agtcacagca caagactttg tgtatagctg
     1861 gcaacgttct gttgatccga acactgcttc tccgtatgcc agttatctgc aatatgggca
     1921 tatcgccggt attgatgaaa ttcttgaagg gaaaaaaccg attaccgatc tcggcgtgaa
     1981 agctattgat gatcacacat tagaagtcac cttaagtgaa cccgttccgt acttctataa
     2041 attacttgtt cacccatcaa cttcaccggt gccaaaagcc gctatcgaga aattcggcga
     2101 aaaatggacc cagcctggta atatcgtcac caacggtgcc tataccttaa aagattgggt
     2161 cgtaaacgaa cgaatcgttc ttgaacgcag cccgacctac tggaacaacg cgaaaaccgt
     2221 tattaaccag gtaacctatt tgcctattgc ttctgaagtt accgatgtca accgctaccg
     2281 tagtggtgaa atcgacatga cttataacaa catgccgatc gaattgttcc agaagctgaa
     2341 aaaagagatc ccggacgaag ttcacgttga tccatacctg tgcacttact attacgaaat
     2401 taacaaccag aaaccgccat tcaacgatgt gcgtgtgcgt accgcactga aactaggtat
     2461 ggaccgcgat atcattgtta ataaagtgaa agcgcagggc gacatgcccg cctatggtta
     2521 cactccaccg tatactgatg gcgcaaaatt gactcagccg gaatggtttg gctggagcca
     2581 ggaaaaacgt aacgaagaag cgaaaaaact gctggctgaa gcgggttata ccgcagacaa
     2641 accgttgacc atcaacctgt tgtataacac ctccgatctg cataaaaagc tggcgattgc
     2701 tgcctcttca ttgtggaaga aaaacattgg tgtaaacgtc aaactggtta accaggagtg
     2761 gaaaacgttc ctcgacaccc gtcaccaggg tacttttgat gtggcccgtg caggctggtg
     2821 tgctgactac aacgaaccaa cttccttcct gaacaccatg ctttcgaaca gctcgatgaa
     2881 taccgcgcat tataagagcc cggcctttga cagcattatg gcggaaacgc tgaaagtgac
     2941 tgacgaggcg cagcgcacag ctctgtacac taaagcagaa caacagctgg ataaggattc
     3001 ggccattgtt cctgtttatt actacgtgaa tgcgcgtctg gtgaaaccgt gggttggtgg
     3061 ctataccggc aaagatccgc tggataatac ctatacccgg aatatgtaca ttgtgaagca
     3121 ctaatggcaa tacgtggggc aggagtgtcc tgctccacgg tgtctgattt ttatcgcatt
     3181 acagaaggca caggccagaa ggtagggcaa tgttaaaatt tattctacgt cgctgtctgg
     3241 aagcgattcc gacgctattt attcttatta ctatttcgtt ctttatgatg cgcctcgcgc
     3301 cgggaagccc ttttaccggc gaacgtactt taccgccaga agtgatggcc aatatcgaag
     3361 agaaatatca tctt
//
LOCUS       DAZFWL020000266         3345 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFWL020000266 DAZFWL020000000
VERSION     DAZFWL020000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.268"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1383
                     /gene="tssI"
                     /locus_tag="ACHKNP_RS25015"
                     /old_locus_tag="pgaptmp_005003"
     CDS             <1..1383
                     /gene="tssI"
                     /locus_tag="ACHKNP_RS25015"
                     /old_locus_tag="pgaptmp_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HGU4706981.1"
                     /translation="QEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQMDGWRNNAET
                     ARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGRQGAGTALENH
                     FAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKFNWDRYNPADQ
                     DSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHHENRTPGSL
                     PGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHA
                     ETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRALTVGVAYQTT
                     VGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAG
                     EHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKPPDE
                     KQDTPDMREY"
     gene            1451..>3345
                     /locus_tag="ACHKNP_RS25020"
                     /old_locus_tag="pgaptmp_005004"
     CDS             1451..>3345
                     /locus_tag="ACHKNP_RS25020"
                     /old_locus_tag="pgaptmp_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HGU4706982.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVQPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQIRDDALILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDLLPAPLPPYRVLTGMADRFGR
                     TLAYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKPHTASLSSPDSPRPL
                     SAPSFPDTLPGTEYGADSGIRLSAVWLMHDPEYPENLPAAPLVCYDWTPRGELAAVYD
                     RSGTQMRHFTYDDKYRGRMVGHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQD
                     RITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTE
                     YGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSR
                     SGETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQ
                     MTAVHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQY
                     DAWGKAV"
BASE COUNT          762 a          907 c         1112 g          564 t
ORIGIN      
        1 caggaaggcc agcaccagga ttaccagcgc acacagtatg aagtgtatga ctaccccgga
       61 cgtttcaagg gggcccacgg gcagaacttt gcccgctggc agatggacgg ctggcgaaac
      121 aatgcagaaa ccgcgcgggg aatgagtcgc tcgccggaga tatggccggg acgacgaatt
      181 gtgctgacgg ggcatccgca ggcgaacctg aaccgggaat ggcaggtggt ggcaagtgaa
      241 ctgcacggcg aacagccaca ggcggtgcca ggacggcagg gagcggggac ggcgctggag
      301 aaccattttg cggtgatccc ggcagacaga acatggcgac cacagccgtt gctgaaaccg
      361 ctggtcgacg gcccgcagag cgctgtcgtg acaggaccgg caggcgagga aatcttctgc
      421 gacgaacatg gtcgcgtgcg ggtgaagttc aactgggacc gttataaccc ggcagaccaa
      481 gacagttcgt gctggatccg tgtggcacag gcgtgggcag gcaccggttt tggccacctg
      541 gcgataccgc gtgtgggtca ggaggtgatt gtggacttcc tcaacggcga cccggaccag
      601 ccgattatta tggggcgtac ctaccaccat gaaaaccgca cacccggcag cctgccgggg
      661 acgaagacgc agatgacgat ccgctcgaaa acctataagg gcagcgggtt taatgaactg
      721 aagtttgacg atgcgacagg gaaagaacag gtctacatcc acgcgcagaa gaacatgaac
      781 accgaggtgc tgaataaccg caccacggat gtgataaaca accatgctga gaccattggc
      841 aacaatcaga tgattgcggt taccaacaat cagatacaga cggtgggcgt taaccagata
      901 gagacggtgg gcagtaacca gatcattaac gtgggttctg ttcaggtgga aacgattgga
      961 cttgttcgtg cgctgaccgt gggcgtggcg taccagacga cggtaggtgg cattatgaac
     1021 acttcggtgg ctctgatgca gtcctcgcag atcggtttgc ataaatcgtt gagggtcggg
     1081 ctgggttatg acgtcaaagt cggaaataac gttaccttca ccgttggtaa aacgaaaaag
     1141 gatgataccg ggcagaccgc gatttactcc gccggtgagc atctggagct ctgctgtggt
     1201 aaggcaaggc tggtgctgac gaaggacgga caaatttttc tcaacggcac aaaaattcat
     1261 ttgcagggta aggagcaggt taatggtgac tcactgttga ttaactggaa ctgtgcagcc
     1321 tcgaaatccc caccgaagcc ccctgatgaa aagcaggata cgccggatat gagagagtac
     1381 tgatcccgta cctgttcgga attcagtaac aaaccagcat taaaaataaa cagaagaagg
     1441 aatttgaatc atgagcggaa aaccagcggc gcgtcaggga gatatgactc agtatggcgg
     1501 tcccattgtc cagggttcgg caggtgtaag aattggcgcg ccaactggcg tggcgtgctc
     1561 ggtgtgtccg ggcgggatga cctcgggcaa cccggtgaac ccgctgctcg gtgcaaaagt
     1621 acagcccggc gagacggacc ttgcgctgcc cggcccgctg ccgttcattc tctcccgcac
     1681 ctacagcagc taccggacga agacgcctgc accggtgggc gttttcggcc ccggctggaa
     1741 agcgccttct gatatccgct tacagatacg cgatgatgca ctgatactca acgacaacgg
     1801 cgggcggagc attcactttg agccgctgct gccgggggag gcggtgtaca gccgcagtga
     1861 gtcaatgtgg ctggtgcgtg gcggtaaggc agcgcagccg gacggccaca cgctggcacg
     1921 gctgtgggcg tcgctgccac cggatatccg gttaagcccg catctttacc tggcgaccaa
     1981 cagcgcacag gggccgtggt ggatactggg gtggtctgag cgggtgccgg gtgctgagga
     2041 cttactgcca gcgccgctgc cgccgtaccg ggtgcttacc gggatggcgg accgcttcgg
     2101 gcggacgctg gcgtaccggc gtgaggccgc cggtgacctg gccggggaaa tcaccggcgt
     2161 gacggacggt gccgggcggg agttccgtct ggtactgacc acacaggcac agcgggcgga
     2221 agaggcccgt aaaccgcaca ccgcttcgtt atcttcccct gacagtcccc gccctctatc
     2281 agctccttcc ttccccgaca cactgcccgg tacggaatac ggcgctgaca gcggtatccg
     2341 tctttcggcg gtgtggctga tgcacgaccc ggaatacccg gaaaatttac ctgccgcgcc
     2401 actggtgtgc tacgactgga cgccacgcgg tgagctggcg gcggtgtatg accgcagcgg
     2461 tacgcagatg cgccacttta cttacgatga taaataccgg ggccggatgg tggggcaccg
     2521 ttatgcaggc aggccggaga tgcgctaccg ctacgacgat gcggggcggg tggtggagca
     2581 acttaacccg gcaggcctga gttaccgcta ccagtatgag caggaccgca tcaccgtcac
     2641 ggacagcctg aaccggcgtg aggtgctgca tacagaaggc ggggccgggc tgaagcgggt
     2701 ggtgaaaaaa gaactggcgg acggcagcgt cacgcacagc gggtatgacg cggcaggaag
     2761 gctcacggcg cagacggacg cggcgggacg gcggacagag tacggtctga atgtggtgtc
     2821 cggcgatatc acggacatca ccacgccgga cgggcgggag acgaaatttt actataacga
     2881 cgggaaccag ctgacggcgg tggtgtcccc ggacgggctg gagagccgcc gggaatatga
     2941 tgaaccgggc aggctggtat cggagacatc gcgcagcggg gagacagtac gctaccgcta
     3001 cgatgacgcg cacagtgagt taccggcgac gacaacggat gcgacgggca gcacccggca
     3061 gatgacgtgg agccgctacg ggcagttgct ggcgttcact gactgctcgg gctaccagac
     3121 ccgctatgaa tacgaccgct tcggccagat gacggcggtt caccgcgagg aaggtatcag
     3181 cctttaccgc cactatgaca accgtggccg gttaacctcg gtgaaagacg cacagggccg
     3241 tgaaacgcag tatgagtaca acgccgcagg cgacctgact gccgttatca ccccggacgg
     3301 caaccggagc gagacacagt acgatgcgtg gggaaaggcg gtcag
//
LOCUS       DAZFWL020000267         3340 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFWL020000267 DAZFWL020000000
VERSION     DAZFWL020000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.269"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(658..855)
                     /locus_tag="ACHKNP_RS25025"
                     /old_locus_tag="pgaptmp_005005"
     CDS             complement(658..855)
                     /locus_tag="ACHKNP_RS25025"
                     /old_locus_tag="pgaptmp_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4706983.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(921..1191)
                     /locus_tag="ACHKNP_RS25030"
                     /old_locus_tag="pgaptmp_005006"
                     /pseudo
     CDS             complement(921..1191)
                     /locus_tag="ACHKNP_RS25030"
                     /old_locus_tag="pgaptmp_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1496..1684
                     /locus_tag="ACHKNP_RS25035"
                     /old_locus_tag="pgaptmp_005007"
     CDS             1496..1684
                     /locus_tag="ACHKNP_RS25035"
                     /old_locus_tag="pgaptmp_005007"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706984.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            2012..2206
                     /locus_tag="ACHKNP_RS25040"
                     /old_locus_tag="pgaptmp_005008"
     CDS             2012..2206
                     /locus_tag="ACHKNP_RS25040"
                     /old_locus_tag="pgaptmp_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706985.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2549..2677
                     /locus_tag="ACHKNP_RS25045"
                     /old_locus_tag="pgaptmp_005009"
     CDS             2549..2677
                     /locus_tag="ACHKNP_RS25045"
                     /old_locus_tag="pgaptmp_005009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706986.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2987..3322)
                     /locus_tag="ACHKNP_RS25050"
                     /old_locus_tag="pgaptmp_005010"
     CDS             complement(2987..3322)
                     /locus_tag="ACHKNP_RS25050"
                     /old_locus_tag="pgaptmp_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706987.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT          991 a          762 c          733 g          854 t
ORIGIN      
        1 gtgagcttat gcacaaaaat aaccaagtat attatcttct ggtagagatt caggatacag
       61 tttgtaggag ctttcttttt tgaacaatga tttgcaggac agttatgcta aactagcgga
      121 atcagtatca caacaataaa tgactgatca tacctgaaat ttgaattaac aggcatcatg
      181 aatgtcaata gtctaagcgg tgactactta tatcgcttgc ataaatacta aacactgact
      241 agaaaagtat tggataaccc cgtcactaaa agtaacgctt cggttatttg ctcggaatgt
      301 attaacgcca agattgttag ccgttacgcc ttatatcact ggcgctgaca aatgtttctt
      361 ttctaattta ctgaaccggc gcagacacgc aggaaagcac gccggcaccg gtgtaatgcc
      421 agttctaatc aataagaatg gaatgacgaa tcagtcagga agggataaca acccgctgct
      481 gaacggaagg tatcagcagg aagatgaacg ggaatatcgc cagcaccaag ggcagggccg
      541 ggttccacaa taccagcgct ggcattttgc acattgccag cgtaccggca ttacaacacg
      601 tttcatgctg ctgcccccca ggattcctcg attcgttgct acatccactg ctctacttca
      661 cttccactgc tccacttcac ttttgtacca gcggctgctg cgccccagtt tgatgggttt
      721 agggaaatgg ccttcactga tcaacttgta aaaccatttg tcggtcatac cggtgaaact
      781 ggtgatatag ttcatgtcga tcagggaatt atcggcacgc agaaatacgc tgtcgtggct
      841 tgttgtagtc aacatggtca gaatgtcctg tgagatttat gaagattgtg cgaatgcggg
      901 aaatctactg agctgtgctt tcagaactgg cctgttacgg gataccaggg attaccggcg
      961 gggtaacggg cttccggatc atacacacca cgattatcgc ggacaaaatc actgaacgcc
     1021 catatcacct ctttaagtat gtcttcgcag tccggtaaat gacgatccag cgccacatcc
     1081 cgggtggtac agctttgatg cgcccggtga cacaaagccc ggattgttcc agacatcctg
     1141 aatcaaacgc cccagattag gggcgtcgaa atatgcctct ctgaccatta tattccggcg
     1201 tataggtagc aggtctgaag tgacaatgcg tcacctgacg ttaaaagtca cccaagatga
     1261 ccccagtggg ggcaacaagt ataaaaccgt tttttgatta agtggtcgga tatgaaaagg
     1321 ggaaggttta tgtgaaaaat acctgtgttt tctttgaaaa cacacaacag tatcaggtta
     1381 tttatgttta tacagtgcgt tccaaactga cctgaataat tacgcaagtt gcaacaataa
     1441 aacatttttg ccgtagtgtg aggtactccg gtaaattgct tcccaggtgc agacaatgac
     1501 taacgaagaa ctacaggcaa tcgcattatc catgcttgag cgccagcgcg aaaaagtaca
     1561 ccagcaacga gagtctttgc ttaacccatc tccattcacc aggccaaaac gattggagct
     1621 tgaagcattc ttggacaaat acccacggcg gcttaaatca ggcaaaacca gaccaccagg
     1681 ctgatagctc acagtttgtt ctccgattca ataaaacgga taagaaccat cagcgcggta
     1741 gcgtaaggca ctacagcgca ttgctttacg agtaataaca atggatttat caccagaaga
     1801 cgcaaagttt ttacgagaac tgcaacaccg tatgctcaga gaagccatta tcgaacagca
     1861 aacgggcatg agcgccatag acccattgcg atacaaacgg gcctcatctc ctttagttga
     1921 cgacaattcc aatgcggata aaacacgtcc accacactga ttacattttt cattagcaca
     1981 gtggtgtgat gcagcatacg aggaaaaccg gatgaatgag aacaaaataa aaaggctgga
     2041 gcaactgtta caggcacgcc aacgggagtt tgccactaaa tggggacgtg ccgcgctgcc
     2101 acatgaacgc ctaacctatt ttccacttga agacctcatt aaaaataatg gcaaaaaaat
     2161 caggtctgat aagaaacact tcttagaatg caatgaagac aaataacaac aatctgcata
     2221 ttgccaccaa ccacagatgt ggcatcgtta tcagcgcact tgattttctt tatatccact
     2281 gacgagtttg ccggtcattc tggtatcatg cagatattta cggcgtacaa gcacactagc
     2341 atgacattgt aataaggcct gagcctaacg ctttaatgtg tgtcatacac tcagtggcgt
     2401 aatcgttgac aatatggtgc atgtaatcgt aagcgtccag cgaaatccat ctggtcataa
     2461 tctgaagcat ccgacaaagt ggtgtccacc aaataagtga tctgcgccca atatcctgag
     2521 ccggaacttt gacaaaggcc aacgaaaaat gggagcggat gttacagagt tctcggtaca
     2581 ggggaaaagt ggtacctgtc gccagttctc cgatctgttt aaccgggaaa ttatctccta
     2641 cagcttgtca gaaaggtcgg tgatggagca tcgttaatac gatgctatac gatgcattca
     2701 cagtgcccgg ccagaggatg ccccgtcgct gcatatggat cagggttggc aatatcgaat
     2761 tgcaggctat aggcaaagtt aaagccccat ggagtagcac aaaatatgcc gcacaaagga
     2821 aacggtctga ataacgcagt gatgaagaac ttcttcagca cactgctaaa acgcatagtg
     2881 atcgagcgct gattctggcg aacaactgaa ctaatacatc agaatctgca ttatgttaaa
     2941 taaatataaa aagatggttt aaataccccg ttacttttga cttacactat acggtatcgc
     3001 atcgtttaat attcgcaccg gccagatttt tatttctatt agttgtcaca atactgaatg
     3061 cgtacgacca cagtattctg gctcctgtgt ggttatgctt taattctgcg ttccgggcag
     3121 ataagcagtt gcttgcagga atccttcttg tgttaatgtc agttcccctt ttaccagtgc
     3181 tgatttccac attccgtcca acagagctta tagcctttcc ctggattata gcattgtccg
     3241 gctgaagttc tttttgaata ataatagaag cactgctggc agatccagtc cgtttttcat
     3301 aacccactgt actgataacc ataatctaat cagtagaaat
//
LOCUS       DAZFWL020000268         3303 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFWL020000268 DAZFWL020000000
VERSION     DAZFWL020000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.270"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..178
                     /locus_tag="ACHKNP_RS25055"
                     /old_locus_tag="pgaptmp_005011"
     CDS             <1..178
                     /locus_tag="ACHKNP_RS25055"
                     /old_locus_tag="pgaptmp_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HGU4706988.1"
                     /translation="ATEFLFLIIFIVLMIPIPPLLLVWFIVKIIEPVIELYNDVVWAS
                     FSTLHNKINPYKES"
     gene            180..545
                     /locus_tag="ACHKNP_RS25060"
                     /old_locus_tag="pgaptmp_005012"
     CDS             180..545
                     /locus_tag="ACHKNP_RS25060"
                     /old_locus_tag="pgaptmp_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGU4706989.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            542..886
                     /locus_tag="ACHKNP_RS25065"
                     /old_locus_tag="pgaptmp_005013"
     CDS             542..886
                     /locus_tag="ACHKNP_RS25065"
                     /old_locus_tag="pgaptmp_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706990.1"
                     /translation="MTTFTREQLIAHAEETIETQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            complement(1091..1390)
                     /locus_tag="ACHKNP_RS25070"
                     /old_locus_tag="pgaptmp_005014"
     CDS             complement(1091..1390)
                     /locus_tag="ACHKNP_RS25070"
                     /old_locus_tag="pgaptmp_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4706991.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDSRKVFIVGIYHGGQSYETDFL"
     gene            complement(1396..1653)
                     /locus_tag="ACHKNP_RS25075"
                     /old_locus_tag="pgaptmp_005015"
     CDS             complement(1396..1653)
                     /locus_tag="ACHKNP_RS25075"
                     /old_locus_tag="pgaptmp_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU4706992.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            1789..2061
                     /locus_tag="ACHKNP_RS25080"
                     /old_locus_tag="pgaptmp_005016"
     CDS             1789..2061
                     /locus_tag="ACHKNP_RS25080"
                     /old_locus_tag="pgaptmp_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4706993.1"
                     /translation="MAHNTKHHNSDIAPASRQGRCSHTFKYDWYQHDPCTEEQAEWLI
                     HNYRRRGYEFKKALSLDYRHWIIYVRLLYSERPPRPSRTFQQRIWR"
     gene            2063..3109
                     /locus_tag="ACHKNP_RS25085"
                     /old_locus_tag="pgaptmp_005017"
     CDS             2063..3109
                     /locus_tag="ACHKNP_RS25085"
                     /old_locus_tag="pgaptmp_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4706994.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAQQNLTAWMIDVIRHAISGAQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQKPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTHWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            3122..>3303
                     /locus_tag="ACHKNP_RS25090"
                     /old_locus_tag="pgaptmp_005018"
     CDS             3122..>3303
                     /locus_tag="ACHKNP_RS25090"
                     /old_locus_tag="pgaptmp_005018"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4706995.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIA"
BASE COUNT          856 a          777 c          880 g          790 t
ORIGIN      
        1 agcgacagaa tttttgtttc tcattatatt tatcgtgttg atgataccga tacccccgct
       61 attactggtc tggtttatcg tgaaaataat tgagccagtt attgaattgt ataacgacgt
      121 ggtgtgggcg tcgttcagca cactgcacaa taaaattaat ccgtataagg aaagctgata
      181 tggcactgac gaaaaaacaa cgtgcagagc tgcgcatgaa gttcggcggt cgctgtgctt
      241 attgcggctg cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt
      301 ttgatggaaa tatgcttcac caggaacgtg acgatatatc caacatggtt ccggcatgcc
      361 acccatgcaa tctgcacaag cattgcagta gtctggaaga ttatcggcga attatcagtg
      421 atggtcgtcg tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggattgg
      481 ttgaaatgaa atctgaccca gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg
      541 catgacgact tttaccagag agcagttaat agctcacgca gaggagacta ttgaaacaca
      601 gagactgtgc ataccgggca caatcgacca tgacatcatc cgcacatata agatggatat
      661 tgctgttctg gaaatcgcac tggtatcgct ggcagcagag ccagccggta aattgcatga
      721 atacaaacca gtgggatatc agcgtctggt cgatgagtta accatgctgg taaagcagtt
      781 aacctggcaa ctgaggaaag cgaagccaga ctgcaaatta ccggataagg cgatgagtta
      841 tctggagcgg aacggactga taagcgtgga ggatatttta cgatgacctg gcctgaagca
      901 ttcacaacgg taggaattgc gatggcggtg gcgctggtgg tgtattcgat ttgccgctgg
      961 ggataaatcg ccgaaaaaag atcccgacat aaacatgagc cgggatcttt gatttatata
     1021 gcctacgaat ccgccagtaa gagagggggc ggacggttaa ttctaacacc ggaatgatgt
     1081 gggtaaaagt ttataagaaa tcggtttcat aactttgccc accatgatag ataccgacaa
     1141 taaagacttt tctgctatca acggcaaaag caataatcgt tctgtggcgg aaatgagtta
     1201 cccgcatccc ctggcgaata tcatcgcgtt tattgccccg atgcgggaat gtagaaaacc
     1261 catcaagata atcaagaagc gcattggcat aattgtcagc aatgacgttc cctgctttct
     1321 ccgttatata cctgtgcagg ttgattattt gttgttcggc ctcaggagta atgatgactt
     1381 catatgtcat gcagattact tcccggatcg aatcgcggcg cgaacctgtg aaatggagcg
     1441 tccgttgttt ggattttcgc ggatagaatc aagagagggg gcggctgaat gcgttaacca
     1501 cgcttcgatt gctttatcgc gctcattcag tgcgcgaagc ccttcacgaa tgacctcgct
     1561 ttctgaagca taggcaccgg aagccacacg ggcgcgcacc atgtcagcca tttcgttagt
     1621 taatgtaatg ctgaattgtt gggttgtacg catggtaaac ctcacggagt aggatagaac
     1681 accattcgat gatagcacgt tgcctgttga cgacaacaga aatcagagac aatattgccg
     1741 cacgccagct tgaacaactg gcacctgctg cgccagcaga gaaaaccgat ggcgcacaat
     1801 accaaacatc acaattctga tatcgcccct gccagcaggc aagggcggtg ttctcacaca
     1861 ttcaaatatg actggtatca gcacgatccc tgcactgaag aacaggccga atggctgatt
     1921 cataactacc gcagacgtgg gtatgagttt aagaaagccc ttagcctcga ctaccgtcac
     1981 tggataatct acgtcaggct cctttattcc gaacgcccgc cgcgtccgtc ccgcacattc
     2041 cagcaacgca tctggaggta acgtgcgggt attacttcga cctgttctgg taccggaact
     2101 cgggctggtg atcgttaagc cgggtcgtga atccatgccg gtattccaca atacccgggt
     2161 actggtggag ccggaaccga aaagcatgcg taatctgccg tccggggttg ttcctgccgt
     2221 tcgccagccg ctagtggaag acaaaacatt gctgccgttt ttcagtaacg cacgggtgat
     2281 tcgtgctgct ggtggtgctg gtgcattgtc tgactggctg ttgcgccata ttaaatcctg
     2341 ccagtggcca cacggcgatt atcatcacag cgaaaccgtc attcaccgtt atggtaccgg
     2401 cgcaatggtg ttgtgctggc actgcgacaa ccagctgcgc gaccagacat ccgaatcact
     2461 cgggcagctt gctcaacaaa atctgacagc ctggatgatt gacgtcatac gtcacgcaat
     2521 aagtggtgca caggaacggg aattatctct ggctgaatta tcctggtggg cggtccgcaa
     2581 tcaggtggcg gacgcgctac cggaagcggt attacgtcgt tcgctggggt tgcgtgcgga
     2641 aaaaatccgc tcaatgtacc gtgaaagcga catcgtaccg ggagagcaga ccgccaccag
     2701 catactgaag cagcgcacaa aaaatcttgc gccgctgcct cacgctcacc agcaaaaacc
     2761 gccacaggaa aagacggtgg tcagcattgc cgttgatcct gagtctccgg aatctttcat
     2821 gaaacgacct aaacgtcgcc gctgggttaa cgagaaatac acgcactggg tgaagacaca
     2881 gccgtgtgcg tgttgtggta agcctgctga cgatccgcat cacctgattg gtcatggtca
     2941 ggggggaatg gggacaaaat ctcacgatat tttcacgcta ccgctgtgtc gggagcatca
     3001 caacgagctt catgcggatc cgctggcgtt cgaagaaaag catggttctc aggttgattt
     3061 aatttttcgt tttcttgatc acgcctttgc aactggcgtg cttgggtaaa agaggtgact
     3121 gatgctcata gatttggttt taccttaccc gccgacggtg aacacttact ggcgacgccg
     3181 tggcagcaca tattttatct cggaggaggg aaagcgttat cgccgggctg tggcgcttat
     3241 tgttcgccag cagcggctga aattaagcct gtccggaagg ctggcgataa aggtgattgc
     3301 aga
//
LOCUS       DAZFWL020000269         3246 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFWL020000269 DAZFWL020000000
VERSION     DAZFWL020000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.271"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            325..846
                     /locus_tag="ACHKNP_RS25095"
                     /old_locus_tag="pgaptmp_005019"
     CDS             325..846
                     /locus_tag="ACHKNP_RS25095"
                     /old_locus_tag="pgaptmp_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HGU4706996.1"
                     /translation="MSHDAEKKIRLCIQCENEALKDSDFCAVCEEREFKKIRGWLYLP
                     AIGLVLTILANMISINFTIRFLMENIAQIGHAQKGILFFELFAFIGMFSYGIFVSSLF
                     FRKKRQLPHHYIALVGIGTIFVAVDLLLGHIYLDVPYVFDTVKPLVRNVFSACIWIPY
                     FIVSERVKRTFVK"
     gene            881..2014
                     /gene="cdiA4"
                     /locus_tag="ACHKNP_RS25100"
                     /old_locus_tag="pgaptmp_005020"
     CDS             881..2014
                     /gene="cdiA4"
                     /locus_tag="ACHKNP_RS25100"
                     /old_locus_tag="pgaptmp_005020"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDI system deoxyribonuclease CdiA-o11"
                     /protein_id="HGU4706997.1"
                     /translation="MVNATLSVVQKNSAFVGSATGELAARAIGMLYPGVKQSDLSEEQ
                     KQTISTLATVSAGLAGGLTGSSTASAAVGAQSGKNAVENNYLSTNQSLTFDKELSDCR
                     KSGGNCQDIIDKWEKISDEQSAEIDQKLKDNPLEAQVIDKEVAKGGYDMTQRPGWLGN
                     IGVEVMTSDEAKAYVQKWNGRDLTKIDVNSPEWTKFAVFASDPENQAMLVSGGLLVKD
                     ITKAAISFMSRNTATATVNASEVGMQWGQGNMKQGMPWEDYVGKSLPADARLPKNFKI
                     FDYYDGATKTATSVKSIDTQTMAKLANPNQVYSSIKGNIDAAAKFKEYALSGRELTSS
                     MISNREIQLAIPADTTKTQWAEINRAIEYGKSQGVKVTVTQVK"
     gene            2014..2517
                     /gene="cdiI4"
                     /locus_tag="ACHKNP_RS25105"
                     /old_locus_tag="pgaptmp_005021"
     CDS             2014..2517
                     /gene="cdiI4"
                     /locus_tag="ACHKNP_RS25105"
                     /old_locus_tag="pgaptmp_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDI system immunity protein CdiI-o11"
                     /protein_id="HGU4706998.1"
                     /translation="MAFNKDQDYWANIFVTPDFLSVETYSGLGMTGRDPLFSPRLLQP
                     DVDDKSLGEEILQALSDSRTLDVLEERVAFFDLEKSKEQYAAWIATLMEKYGYRTKRA
                     LFKNMKKVGIHLVNDVITIRPSFHEKLEAWSGNRINESDYVVLPADSSPTEIGSGLRL
                     ALSRCKG"
     gene            complement(2914..>3246)
                     /locus_tag="ACHKNP_RS25110"
                     /old_locus_tag="pgaptmp_005022"
     CDS             complement(2914..>3246)
                     /locus_tag="ACHKNP_RS25110"
                     /old_locus_tag="pgaptmp_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4706999.1"
                     /translation="PGRALHKAIVYALSHRVELSRFLEDGAVPLDNNVCERAIKNVVL
                     GRKSWLFAGSQMAGERAAQIMSLLETAKRNGLEPHAWLTDVLMRLPEWPEERLAELLP
                     LEGFTFSG"
BASE COUNT          907 a          660 c          828 g          851 t
ORIGIN      
        1 agcagggatt ggtatttgga taaaagcaaa gttacaagaa agaaaagacg ataaaaaata
       61 ggcttacggt aacttcaact ttggggaatt tttattggta gttatgttct tggtcaatgc
      121 catcaggaat gaggtaatgt gcgtattacc gtggaagtga tcggtgagta actccaatct
      181 caatagaggt tattgggcgc tctccttcca tgaaaacacg caacctaatc ggggaccggg
      241 gcatatgctg actggtgaca atacagatga aaataaacag catgatcttc aacttttccc
      301 gtataaataa taggtgatta tcagatgagc catgatgcag agaagaaaat aagactttgt
      361 attcaatgtg agaatgaagc actgaaggac agtgactttt gcgctgtttg tgaagaacgc
      421 gaatttaaaa aaattcgtgg ttggctatac ttgcctgcaa taggactggt tttaacaatt
      481 ctcgctaata tgatctctat taatttcact atacgattct taatggaaaa tattgcgcag
      541 atcggtcacg cacaaaaggg gattctcttt tttgaactct ttgctttcat tggtatgttt
      601 tcctatggaa ttttcgttag tagtttgttt tttcgtaaaa agcgtcaatt gcctcatcac
      661 tatattgcac ttgttgggat aggaacgata tttgtcgctg tcgatttact gctgggccac
      721 atctatttag acgtgcctta tgtttttgat acggtaaaac cattggtacg taatgtgttt
      781 agtgcctgta tttggatccc ctatttcatt gtatctgagc gcgttaagcg gacatttgtg
      841 aaataatttg tccgtcagat gtcccacatg aactcatgca gtggtgaatg ccacgctgtc
      901 ggtggtacag aaaaatagcg cctttgtcgg ctcagcaaca ggagaactgg cagccagagc
      961 gatcggaatg ctgtatccgg gcgtgaaaca gtcagacctc agtgaagagc agaagcagac
     1021 gataagtacg ctggcaaccg tgtcagcagg gctggccggc ggtcttaccg gaagcagcac
     1081 cgcatcagca gcagtgggag cacagagcgg gaagaatgct gttgagaata attaccttag
     1141 cacaaaccag tctctgacct tcgataaaga attgtctgat tgtcggaaat ctggaggtaa
     1201 ctgccaggac attattgata aatgggaaaa aatcagtgat gaacagagtg cagaaatcga
     1261 tcagaagctg aaagacaatc cgctggaagc ccaggttatt gataaggaag ttgcgaaggg
     1321 cggatatgat atgacgcagc gcccaggttg gctgggtaac attggtgtgg aagtaatgac
     1381 cagtgatgaa gccaaagcct atgttcagaa gtggaatgga cgggatctga caaaaataga
     1441 tgtaaacagc cctgaatgga ctaagtttgc agtgtttgct tctgatcctg aaaatcaggc
     1501 catgctggtc agcggaggtt tgctggtaaa agatataact aaagcggcta tatcattcat
     1561 gagtcgcaat acagcgacag cgacggtcaa cgcatcagaa gttggaatgc agtggggaca
     1621 gggaaatatg aaacagggta tgccgtggga ggattatgtt gggaaatctc ttcccgctga
     1681 tgcccgttta ccaaagaact ttaagatctt tgattactat gatggtgcta ccaaaactgc
     1741 taccagtgta aaatcaatag atacgcaaac tatggcgaaa ttggctaatc cgaatcaggt
     1801 ttacagttcg ataaaaggaa acatcgatgc tgcggctaag tttaaagaat atgcactctc
     1861 tgggcgagaa ttaacttcat caatgatctc taacagggag atacagctcg ctattcctgc
     1921 tgacacaact aaaacacagt gggcagaaat aaacagggct attgagtacg gtaaatctca
     1981 gggcgtcaaa gtgacggtaa cgcaggtgaa ataatggctt ttaataaaga tcaggattac
     2041 tgggcgaata tatttgttac accagatttt ttgagtgttg aaacttattc aggattagga
     2101 atgacaggca gagatccatt gttttcacca cgtttattac agcctgatgt agatgataaa
     2161 agcctaggtg aggagatcct acaggcattg tctgacagcc gtacactaga tgttttggaa
     2221 gagcgagtag cttttttcga tcttgaaaaa agtaaagaac aatatgcagc ttggatagct
     2281 actctgatgg agaagtatgg ttatagaact aaacgtgcat tatttaaaaa catgaaaaaa
     2341 gttggtatcc atctggtaaa tgatgttatc acaatccggc cttcattcca tgaaaagctg
     2401 gaagcatgga gtggtaacag gatcaatgaa tctgactatg tggtgcttcc tgcagacagt
     2461 tccccaaccg aaatcggctc aggtcttcgc ctggccttga gtcgctgcaa aggttaacct
     2521 agatatcccg cccatcactg gcgggatttt tgtgtgcttc caagcatatc atcttgacgc
     2581 gtttttcggg cgcgtgtcat cggcggcgca tacgaatgat cgcgatctga cgatggttga
     2641 tttattttgt tgcgctgggg cgatcgccag acaggtaccg gaggcgggct ctgaagtact
     2701 gagtagggtg atcgcgtcag gccgcgaagt actgactcgg ggattaatgg caggtgatga
     2761 tgtactgacc cgggaatgac tccgggctga ttcaggttct tattcgctgt cagcccgtgt
     2821 ggttgctggt ctggctcatt tcctgtcgtc aggagttcaa ctcccgactg caggttatgg
     2881 cccggtgcac gaacacctga cggcaggtat cactcacccg gagaaggtaa atccctcaag
     2941 aggcagcaac tctgccagtc gctcctccgg ccactccggc agacgcatca ggacgtctgt
     3001 cagccaggca tgcggctcca gaccgttgcg tttcgcggtt tccagcaagc tcattatttg
     3061 cgcggcgcgt tctcctgcca tctgcgaacc ggcgaacagc cacgattttc tgcccaggac
     3121 cacgtttttg atggcccgtt cacacacatt attatccagc ggcaccgcac catcttccag
     3181 gaagcggctt agttccacgc gatgagacag cgcatagaca atggctttgt gtaatgccct
     3241 tcccgg
//
LOCUS       DAZFWL020000270         3203 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFWL020000270 DAZFWL020000000
VERSION     DAZFWL020000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.272"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            161..1843
                     /gene="ydeN"
                     /locus_tag="ACHKNP_RS25115"
                     /old_locus_tag="pgaptmp_005023"
     CDS             161..1843
                     /gene="ydeN"
                     /locus_tag="ACHKNP_RS25115"
                     /old_locus_tag="pgaptmp_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4707000.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1895..3052
                     /gene="ydeM"
                     /locus_tag="ACHKNP_RS25120"
                     /old_locus_tag="pgaptmp_005024"
     CDS             1895..3052
                     /gene="ydeM"
                     /locus_tag="ACHKNP_RS25120"
                     /old_locus_tag="pgaptmp_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU4707001.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKNLFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3018..3140)
                     /locus_tag="ACHKNP_RS25125"
                     /old_locus_tag="pgaptmp_005025"
     CDS             complement(3018..3140)
                     /locus_tag="ACHKNP_RS25125"
                     /old_locus_tag="pgaptmp_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707002.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKGRVINYSPFVLQHP"
BASE COUNT          971 a          693 c          642 g          897 t
ORIGIN      
        1 acttaataga acagaaagta atgtcaacgc tttatggact gttatttccc tttttttagc
       61 taaatctgct atctctttat gtgactaact tcacttacat ccacttattt ctcttcgtaa
      121 aattacttcg gaattaagta caataagaag aggaacattt atgaagtctg cattaaagaa
      181 aagtgtcgta agtacctcga tatctttgat actggcatct ggtatggctg catttgctgc
      241 tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca aacgttgctt tctcagactt
      301 tacgccgact gaatacagta ccaaaggaaa gccaaatatt atcgtactaa ccatggatga
      361 tcttggttat ggacaacttc cttttgataa gggatctttt gacccaaaaa caatggaaaa
      421 tcgtgaagtt gtcgatacct acaaaatagg gatagataaa gccattgaag ctgcacaaaa
      481 atcaacgccg acgctccttt cattaatgga tgaaggcgta cggtttacta acggctatgt
      541 ggcacacggt gtttccggcc cctcccgtgc cgcaataatg accggtcgag ctcccgcccg
      601 ctttggtgtc tattccaata ccgatgctca ggatggtatt ccgctaacag aaactttctt
      661 gcctgaatta ttccagaatc atggttatta cactgcagca gtaggtaaat ggcacttgtc
      721 aaaaatcagt aatgtgccgg taccggaaga taaacaaacg cgtgactatc atgacaactt
      781 caccacattt tctgcggaag aatggcaacc tcaaaaccgt ggctttgatt actttatggg
      841 attccacgct gcaggaacgg catattacaa ctccccttca ctgttcaaaa atcgtgaacg
      901 tgtccccgca aaaggttata tcagcgatca gttaaccgat gaggcaattg gcgttgttga
      961 tcgtgccaaa acacttgacc agccttttat gctttacctg gcttataatg ctccgcacct
     1021 gccaaatgat aatcctgcac cggatcaata tcagaagcaa tttaataccg gtagtcaaac
     1081 agcagataac tactacgctt ccgtttattc tgttgatcag ggtgtaaaac gcattctcga
     1141 acaactgaag aaaaacggac agtatgacaa tacaattatt ctctttacct ccgataatgg
     1201 tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa aaaggctata agagtcagac
     1261 ctatcctggc ggtactcaca ccccaatgtt tatgtggtgg aaaggaaaac ttcaacccgg
     1321 taattatgac aagctgattt ccgcaatgga tttctacccg acagctcttg atgcagccga
     1381 tatcagcatt ccaaaagacc ttaagctgga tggcgtttcc ttgctgccct ggttgcaaga
     1441 taagaaacaa ggcgagccac ataaaaatct gacctggata acctcttatt ctcactggtt
     1501 tgacgaggaa aatattccat tctgggataa ttaccacaaa tttgttcgcc atcagtcaga
     1561 cgattacccg cataacccca acactgagga cttaagccaa ttctcttata cggtgagaaa
     1621 taacgattat tcgcttgtct atacagtaga aaacaatcag ttaggtctct acaaactgac
     1681 ggatctacag caaaaagata accttgccgc cgccaatccg caggtcgtta aagagatgca
     1741 aggcgtggta agagagttta tcgacagcag ccagccaccg cttagcgaag taaatcagga
     1801 gaagtttaac aatatcaaga aagcactaag cgaagcgaaa taactaaacc ttcatgcggc
     1861 ggaattttcc gccgccttat tgagcgagat agcgatgcac gttacagcca agccctccag
     1921 ttttcaatgt aatctcaaat gtgattactg tttttacctt gaaaaagagt cgcagtttac
     1981 tcatgaaaaa tggatggatg acagcactct gaaagagttc atcaaacaat atatcgcagc
     2041 gtctggcaat caggtctatt ttacctggca aggcggtgaa cccactctgg ctggcctgga
     2101 ttttttccgt aaagttattc actatcaaca acgctatgca ggccaaaaac gtatttttaa
     2161 tgcattacaa acgaatggca ttttattgaa taatgaatgg tgtgcttttc tcaaagaaca
     2221 tgagtttctg gttggtatct cgatcgatgg cccccaggag ctacatgacc gttacagacg
     2281 cagtaattca ggtaacggta cttttgcaaa agtgatagca gccatcgagc gtctgaaatc
     2341 atatcaagtt gagtttaata cgttaaccgt cattaataac gttaatgtcc attaccctct
     2401 tgaggtttat cattttttaa aatctatcgg cagtaaacat atgcaattta tcgaattgct
     2461 cgaaaccggg acgccgaata ttgatttcag tggtcatagt gagaacacat tccgtatcat
     2521 tgatttttct gtgcctccca cggcttatgg caagtttatg tcaaccattt ttatgcaatg
     2581 ggttaaaaac gatgtgggtg aaattttcat tcgtcagttt gaaagctttg tcagccgttt
     2641 tttggggaat gggcatacca gttgtatttt ccaggagtcc tgcaaggata atctggttgt
     2701 tgaaagtaat ggagacattt acgaatgcga ccattttgtc tatccacagt acaaaattgg
     2761 aaacattaat aaatctgaac tcaaaacgat gaacagtgta caactgacag cgcaaaaaaa
     2821 acggatttca gcgaaatgtc agcaatgtgc atataaacct atctgcaatg gcggttgtcc
     2881 taagcatcgt attactaaag taaacaatga gactgtttct tatttttgcg aaggttataa
     2941 aaaccttttt tcaaccatgg taccttatat gaacgccatg gtggagttag ctaagaacag
     3001 agtaccgctt tatcacatta tggatgttgc aaaacaaatg gagaataatt aataacacgc
     3061 ccttttagct ccattcttag cctccttctt atgaccttaa caaggcaccc gacataaatt
     3121 cctgtcgggt gtttcgtcat catcatcttc ccttgcgaaa atcctggctt acgtggtatg
     3181 cgtcagggca ttagcaatac cct
//
LOCUS       DAZFWL020000271         3177 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFWL020000271 DAZFWL020000000
VERSION     DAZFWL020000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.273"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..851
                     /locus_tag="ACHKNP_RS25130"
                     /old_locus_tag="pgaptmp_005026"
                     /pseudo
     CDS             1..851
                     /locus_tag="ACHKNP_RS25130"
                     /old_locus_tag="pgaptmp_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
     gene            complement(891..1183)
                     /locus_tag="ACHKNP_RS25135"
                     /old_locus_tag="pgaptmp_005027"
                     /pseudo
     CDS             complement(891..1183)
                     /locus_tag="ACHKNP_RS25135"
                     /old_locus_tag="pgaptmp_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(1263..2884)
                     /locus_tag="ACHKNP_RS25140"
                     /old_locus_tag="pgaptmp_005028"
                     /pseudo
     CDS             complement(1263..2884)
                     /locus_tag="ACHKNP_RS25140"
                     /old_locus_tag="pgaptmp_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          782 a          832 c          759 g          804 t
ORIGIN      
        1 cttattgctg cgctgataat atggaccgca aacctattga agatgtgatc ttcgaaatta
       61 ataactttat ctcgctcggt ggtcgcacca ttgttgatgc cacagggtct gagtctatcg
      121 gacgagatgc gcaggcatta cgagaagtcg cattaaaaac aggtctgaat attgttgctt
      181 cctccggtcc ctacctggaa aaatttgaaa gccagagaat tcataaaacg gttgatgaac
      241 tggcggcaac gattgataaa gaattgaatc aagggattgg cgatacggat attcgtgccg
      301 gaatgatcgg tgaaataggt gtctcaccga catttaccga agccgagcat aacagcttgc
      361 gggctgcctc gctggcacag attaacaatc ctcatgtggc gatgaatatt cacatgccgg
      421 gctggcttcg tcgcggtgat gaagtactcg acattgtgtt aggtgaaatg ggcgtctcgc
      481 caaataaagt ctctctcgca cactcggatc cgtcaggaaa agacgtggcg tatcagcgga
      541 aaatgcttga taagggtgtc tggctggaat tcgacatgat tggcctagac attaccttcc
      601 cgaaagaggg aatagcgcca ggggtgcagg agactgccga tgccgtcgct catctcattg
      661 agttgggata cgccgatcag cttgttctca gccacgatgt cttccttaaa caaatgtagg
      721 ctaaaaatgg cggtaatggc tggggatttg ttccagatgt ttttctggcc tatctggcgg
      781 agcgcggcgt cgataaaacg atcctcaaaa aactctgtat cgataatccc ggacgattat
      841 tgaccgcata aaaccattat cccccggcac actgcgccgg gggatgtcaa ttaatacccc
      901 gccgttaaat catccaccaa gcgcgagtcg gatgcgccgt acaactcacc atccgaccca
      961 accataatgc tttgcgtgct gcccatcgcc tcattcagcg ccactttctg accttttgct
     1021 tccagcagct tgagcgtatc cggactaaac ccttttcaac acgcagctcg tccggcaacc
     1081 actgatggtg gaaacgaggc gcattggtcg cttcggcaac attcattcca caattgatgc
     1141 tgttcaccac catttgcagc acggtagtga tgattcagct accggtaaca aagtgagtac
     1201 ataaaggcca tactgtcacg aacagtgcaa aagtggctct tccgcaacct aggtgaagat
     1261 tttcatgcca gtggactcat taccctccgc ctaagtagtt caaatccggc tcgaccatac
     1321 atctggcgta tcagcacctt caggcgattt acatgacctt cgacaacacc attactccag
     1381 cgactgctga ttgcctcaca tatagccgca gcatcagctt ccatccccgc agctacgcac
     1441 cgaagttctg ctgagccgct ttcgtggact cgagtgaacc agctgcttag ctgtgattta
     1501 ttttaggttt tcagaatacg gtagaactcg agtaccagtt gctgtgctat tttcaactcc
     1561 ggttcttttt cacacatcag actaataaat cgggatgcat aattttcttc gcccctgatt
     1621 attctccagg gcatcaaccc gcggcttacc cggaacactg aaggaagtct tgcggcagta
     1681 gtaaccggtg ggttccagcc tttacgccat ttggcaacag tatccctgac gatggtttca
     1741 ctgcctgtcc ccccccctgg gccaccattt cccgccatat ccggctggca ttataattac
     1801 cactttctcg ttgctctttt aaccactccc tccatgggtc cagaagccct cgctttggag
     1861 gccgggttga catttcaggg aatgttcctg aacgcaccca gcgactgact gtcactcgcg
     1921 acaggcctgt aatacgggat atttccctga aactacatcc cttgttatgc agggccataa
     1981 cctctgtcca atgctgatgc cgttttttgc gggtttgctg tttaaggcgt tccggacgac
     2041 ggagcgatgc tacaggcaca gatatttctg gctcaggtga tttcttcagt gataactctc
     2101 tgacaacaag acgtatcaga ggcatatgtc tgtacatcat tcgttcaggc tcatcaccaa
     2161 tattttttag caggtgccat cgatcggcca cctgtctggc ctgaggtgca ccttcacgtg
     2221 ctgctgtcgc atagactcca ctgcgatcac gcgagacaac ctgtatttcc ggatattttc
     2281 tgaaccaggt cgccagcgta cgctgatcac ggccgggaag caggacgagg ggacggtgag
     2341 tatcaagatt gactattaac ataccgcagc agtggccccg atgccacgcc cactcatcaa
     2401 taccgacatg aggcgcgcct gactgtttcg tctccggggt attgataacc ctgcgaagaa
     2461 gagtatcagc actgcaacgg agacccgctg ccgttgcagc ccgttttcca gcctcacctc
     2521 cagctattaa tcccagttga cgttgtaaat tttgtaacgc ctgtgaagac tgttggtgtg
     2581 aaccggcgaa gggagcaagc gactcggcaa aaattttacg tgaacaagct gggttacggc
     2641 agtaccagtg gcggacggaa aatacaagcc atagcgtctg tcctgatcag ggtagatgct
     2701 gtatccggcg ccgacaacaa ctatgaacag agtcgctacg cttaaagcat tcaggacatt
     2761 gtgctgtttt gcgttttccg tggagatgga gggttatacc gtcagagcta ataatttgtc
     2821 gactgcatcg ccatccgcaa ggtaactgta gaagtgtctt aagagagtgc attgggttgc
     2881 ccataactta aaacagctta gatctttgtt cgccagaaag tttcatccat tttcttccac
     2941 ccaggttgcg gaagaaccac ttttcatccg tcgttgacag atgtgaagta tgcgggagaa
     3001 aaagtgaata tctaactgta ataactcaga ccggtacgag tactcggggc gtctacaatc
     3061 attcagggca aagtgctaaa atttctctat cccgggatgc ccctaccaac gcagcactcc
     3121 cggagatatc attacagctc tgcaactgat ttccgcgact gaaccgctca gttccta
//
LOCUS       DAZFWL020000272         3168 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFWL020000272 DAZFWL020000000
VERSION     DAZFWL020000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.274"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..81
                     /locus_tag="ACHKNP_RS25145"
                     /old_locus_tag="pgaptmp_005029"
     CDS             <1..81
                     /locus_tag="ACHKNP_RS25145"
                     /old_locus_tag="pgaptmp_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707003.1"
                     /translation="GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            114..332
                     /locus_tag="ACHKNP_RS25150"
                     /old_locus_tag="pgaptmp_005030"
     CDS             114..332
                     /locus_tag="ACHKNP_RS25150"
                     /old_locus_tag="pgaptmp_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HGU4707004.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            334..597
                     /locus_tag="ACHKNP_RS25155"
                     /old_locus_tag="pgaptmp_005031"
     CDS             334..597
                     /locus_tag="ACHKNP_RS25155"
                     /old_locus_tag="pgaptmp_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707005.1"
                     /translation="MATLTKKERAWLNELQEVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            608..733
                     /locus_tag="ACHKNP_RS25160"
                     /old_locus_tag="pgaptmp_005032"
                     /pseudo
     CDS             608..733
                     /locus_tag="ACHKNP_RS25160"
                     /old_locus_tag="pgaptmp_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            944..1039
                     /locus_tag="ACHKNP_RS25165"
                     /old_locus_tag="pgaptmp_005033"
                     /pseudo
     CDS             944..1039
                     /locus_tag="ACHKNP_RS25165"
                     /old_locus_tag="pgaptmp_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1289..1477
                     /locus_tag="ACHKNP_RS25170"
                     /old_locus_tag="pgaptmp_005034"
     CDS             1289..1477
                     /locus_tag="ACHKNP_RS25170"
                     /old_locus_tag="pgaptmp_005034"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4707006.1"
                     /translation="MPDTKTAVLVAVEFDRKGDWRMKWATYIPGHPDANDGWIIPGAS
                     WKPSHWMPLPEPPQEVRQ"
     gene            1593..1697
                     /locus_tag="ACHKNP_RS25175"
                     /old_locus_tag="pgaptmp_005035"
     CDS             1593..1697
                     /locus_tag="ACHKNP_RS25175"
                     /old_locus_tag="pgaptmp_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707007.1"
                     /translation="MSDDISLAMEGALAVVAVVGVYCLVVFLMDRLGN"
     gene            1943..2098
                     /gene="hokD"
                     /locus_tag="ACHKNP_RS25180"
                     /old_locus_tag="pgaptmp_005036"
     CDS             1943..2098
                     /gene="hokD"
                     /locus_tag="ACHKNP_RS25180"
                     /old_locus_tag="pgaptmp_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU4707008.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVF
                     TAYEPEE"
     gene            2266..2544
                     /locus_tag="ACHKNP_RS25185"
                     /old_locus_tag="pgaptmp_005037"
     CDS             2266..2544
                     /locus_tag="ACHKNP_RS25185"
                     /old_locus_tag="pgaptmp_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707009.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            2546..>3168
                     /locus_tag="ACHKNP_RS25190"
                     /old_locus_tag="pgaptmp_005038"
     CDS             2546..>3168
                     /locus_tag="ACHKNP_RS25190"
                     /old_locus_tag="pgaptmp_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4707010.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMRVFHGGRVLVESEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSVYRESDIVPG"
BASE COUNT          826 a          730 c          848 g          764 t
ORIGIN      
        1 ggaaacgcac caaattattg ctggtgggag gttgatccgg ttgatgctgc aaatgaagcc
       61 atgagttacc acaacgatta atgtcgggag gccgcccgaa agggcggtaa gaaatgacta
      121 cattattcag aaaagaatat ccgcaaaaaa gtagggcgac agaatttttg tttctcattc
      181 tgtttatcgt attgatgata ccgatatccc ctctaatttt tgtctgggca atcgggaaaa
      241 taattgagcc agttactgaa ttgtataccg acgttgtatg ggcgtcgttc aacacactgc
      301 acaataaaat taatccgtat aaggaaaact gatatggcaa ctttgacaaa aaaagaacgg
      361 gcatggttaa acgaattaca ggaagttctt gatcgctgtc catcaccgaa aaaaattggc
      421 ttttacacca ttggcgataa aagcatttac ctgtatgacc tacgccgcat ggatgaaatc
      481 atggaggctc ttgataatcg ttcgtcgatg gattggtgtg ttgctgttca tgatatgaat
      541 gcagggtttg atgaaaagat tttgttcccc tcatcagttg aaagcactgc gggttaagga
      601 gtaacacatg accactatta ccaaagaacg tattgaattg ttcattaaaa atccggttga
      661 aaacgggctt acccgtggtg aacaaatgga actggcacgg attgcgctgg catcgctgga
      721 agcagagccg gtggcaaaga ttatagctca ttacccatta ggggttgacg taggcaaaca
      781 aaaattcgta caggccatta gagagcttcc tgactttggc ggatatctat ttgccgcccc
      841 tccagcgccg atagtgccgg aagaaatgta ttggcaggat gcgccagttg aaggcagcag
      901 caaagcggct gcatacgcta caggctggaa cgattgccgc gaagccatgc ttcagtccgg
      961 aaactttcgg gaaaataaag attcgtcaac caataatttt cggaaaatcc cggaagcgtc
     1021 aaccagctct ccggtaactc cggctcttct gcctggtggt ttcaccattg aggaggcgaa
     1081 ggaattacat gaagacctgg tacgcagcca cataagcaag gccttaagtg gcgaaaagat
     1141 gaaaaagaaa gatcgcgatg ctgatttgcg ctggattcat ggcgttatag ttcaggcagc
     1201 gtggtttgta aaagcatcac tggagcagaa tgcactatcg ggcaactatc cggtaactcc
     1261 ggatagttgg ataagctgta gtgagcgaat gccggatacc aaaacagccg ttcttgttgc
     1321 cgtggagttt gacaggaaag gtgactggcg aatgaaatgg gcgacttaca tcccgggtca
     1381 tcctgacgct aatgatgggt ggataattcc tggtgcgtcg tggaaaccgt cacactggat
     1441 gccgctacca gaaccgccgc aggaggtgcg ccaatgaact ggcctgaagc atttgcaatt
     1501 acaggcgttg ctatggctat cgctttttta gtatatgtta tttgtcggtg ggggtaaaaa
     1561 cgttcgccgg gattaacacc aaaggaggga atatgtcgga tgatatatca ctggcaatgg
     1621 aaggtgcgct ggctgttgtt gctgttgtgg gcgtttactg cctggttgtg tttttgatgg
     1681 atcgactagg gaactgaatt cattacgata tgggaattcc catatcgggt aaaaacggtt
     1741 tgcggtaaag cgagagttaa gtagaattgc tgcgggtgct tgaggctgtc tgcctcgggc
     1801 atgccaccgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc tgcaagacgc
     1861 ttaacattaa tctgaggccc aatctatgct tcacaaacgt aggttagcct cttacgtgcc
     1921 gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt
     1981 catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct gcgaggtacg
     2041 agtccgaacc ggccagacgg aggtcgctgt cttcacagct tacgaacctg aggagtaaga
     2101 gccccggcgg gggagaaatc cctcgccacc tctgatgaat caggcatcct caacgcaccc
     2161 gcacttaacc cgcttcggcg ggttttgttt tttcctggca ttctggttta caattcgcac
     2221 gccagcctga acaactggca cctgctgcgc cagcagagac aaccgatggc gcacgatacc
     2281 aaattataca attctgatga ttctgccgtc tttgccagca ggcgcggacg gtgttttcac
     2341 gcattcaaat ctgactggta ccagcatccc ccatgcactg aagaacaggc cgaatggctc
     2401 attcagtgtt accgcaggcg cggatgcgag gttaaaaaag cccttagcct cgactaccgt
     2461 cactggataa tctccgtcag gctcccttac tccgaacggc caccgcgtcc gtcccgcaca
     2521 ttccagcaac ggatctggag gtaatgtgcg ggtattactt cgacctgttc tggtaccgga
     2581 actcggtctg gttatcgtta agccaggccg tgaatccatg cgggtatttc atggcggcag
     2641 ggtgctggtg gagtctgaac cgaaaagcat gcgtaatctg ccgtccgggg tcgttcctgc
     2701 cgttcgccag ccgctggcgg aagataaatc attactgcca tttttcagca atgagcgtgt
     2761 gattcgtgct gctggtggcg ctggtgcact gtctgactgg ttattacgtc acgttaaatc
     2821 ctgccagtgg cctcatggtg attatcatca cagcgaaact gtcatacatc gttacggtac
     2881 cggcgcgatg gtgttgtgct ggcactgcga caaccagctg cgcgaccaga catccgaatc
     2941 acttgagcaa cttgctcaac aaaacctgtc agcatggatg attgacgtca tccgccacgc
     3001 aatgaatggc atacaggaac gggaattatc gctggctgaa ttatcctggt gggcagtctg
     3061 caatcaggtg gtggacgcat tacctgaggc agtatcgcgt cgttctctgg gattaccggc
     3121 ggaaaaaatc cgctccgtat accgtgaaag cgacatcgta ccgggaga
//
LOCUS       DAZFWL020000273         3162 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFWL020000273 DAZFWL020000000
VERSION     DAZFWL020000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.275"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            363..1340
                     /locus_tag="ACHKNP_RS25195"
                     /old_locus_tag="pgaptmp_005039"
                     /pseudo
     CDS             363..1340
                     /locus_tag="ACHKNP_RS25195"
                     /old_locus_tag="pgaptmp_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1863..>3162
                     /locus_tag="ACHKNP_RS25200"
                     /old_locus_tag="pgaptmp_005040"
     CDS             1863..>3162
                     /locus_tag="ACHKNP_RS25200"
                     /old_locus_tag="pgaptmp_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HGU4707011.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESKSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSDELKTKYANEIIEIKRIMGEYNLLPDKNSRNGLKLLQKQADL
                     LKIIMEDTSVTENIFKNIEMAIADIKKEYYSHTVDIEKNIHAIWVAGSPPESISDYIK
                     TFLKTYKEFTYYLWVDEKAFGAAKFTSVLKQIAFDLACRTIQQNTPQKNIDFINLYNE
                     IRKKYNNNPSGQQEYLNKLRELYATYQKISTPLKHMFNSFF"
BASE COUNT         1074 a          631 c          645 g          812 t
ORIGIN      
        1 tgtgtagggg ggctaaaatt aattctattt cattatatga gaacaccaat aagaataata
       61 atatttgatc acaactgaag atgcttttta caatgaagaa ttattgttaa aaaacgcttt
      121 gttgaatcaa ccgaactgtt gctgagttgc aggatcacat cacgtatttt cctgaaaacg
      181 caggctgctt agtagcaaag cagaagttca aaatcaccaa ctaaatagct gcgccgaata
      241 gtagatcaat gaaagggaac tcagcccgga ttgtgcgatc tgatcaatcg ccaaaaatcc
      301 ccccctaacc ggactgagcg atgccgatca tagcaccaat tccccgcgac gaatgacgcc
      361 tgatgcaaaa agcaatctat aaaactcacg ataaaaatta tgcccgcagg ctgaccgcca
      421 tgctgatgct gcaccggagc gctcgcgtca gtgacgttgc cagaacgctc tgctgcgccc
      481 gttcctccgt caggcgctgg attaactggt tcacgctgtc gagtgttgaa ggtctgaaat
      541 cattacccgc aggacgttcc cgccgctggc cgtttgagca tatctgcaca ttgtttcgtg
      601 aactgataaa acactctccc ggatattttg gctatcagcg ttcacgctgg agtacagaac
      661 tgctggcaat aaaaatcaac gagataaccg agtgccagtt acatgccgga accgttcgcc
      721 gctggttgcc attagtcggg cttgtgtggc gcagggctgc gccaaccctg cgtacccgta
      781 acccgcataa agcagcaatt cacaaagcac tggacgaatg tagtgcggag catccggtct
      841 tttatgaaga tgaagtgtat atctatctta atcccaaaac cggtgcggac tggcaacttc
      901 gcagacagca aaaacatgtg gtaacaccgg ggcagaatga aaaatactat ctggctggtg
      961 ctttacatag tggaacgggc aaagtcagct atgtgggtgg caacagcaaa agctcagcct
     1021 tatttatcag tctgttgaaa catctgaaat tgacataccg acgggataaa cctatcacgc
     1081 tcatagtgga caactacatc atccataaaa gtcgggaaac acagcgctgg ctgaaagaaa
     1141 atccgaagtt cagagtgatt tatcagccag tttactcgcc gtgggtgaat catgttgagc
     1201 ggttatagca ggcgcttcac gatacgataa cgcgcaatca tcagtgccgt tcaatgtggt
     1261 aactgcttaa aaaggttcat tattttatgg aaaccgtgag cccattcccc ggtggtaaat
     1321 atgggctgtt aaaagtgtag cggtattcgg cgcagctatt taggatggtc aggtcgcaga
     1381 agttctgtgc cactacagtg cgtgtactaa acaaaatgac gagggcacga atgtcataat
     1441 acgtgcgtat tacctgcagt agctaccaac tgagggggct tatcccaaac ctgatttatt
     1501 caacaaagct ggactggact tatccttact ctgaattatt ttccagacat atgcatcatg
     1561 tactggctcg cttggttcag gaaatacagg agcaaatcta cagctattag atttattcct
     1621 atttgtatgc aaagtcgcat tgcataatgc ggagctatgt tacagatacg cctgacgtgc
     1681 gtagtaaaat tataaagcaa catatatata caaggagaaa ccgagaaaaa taaatgagtc
     1741 gtatgttggg tagcatcgag ataaatattt ttttatcaca ttagcacctt ggttaagggt
     1801 gttaataata agattttctc acttattaaa acataaataa ttttttaagc ggaaatgtaa
     1861 ttatgagact gccagagaaa gttctttttc ctcctgtcac tagtggcctg tcagggcagg
     1921 aaaaacaaaa aaaaccgaag agcattaccg gatttcagga aaattatcaa cgcaatatca
     1981 ggccaatcaa aacagcatca gaagcccgac tacgcttctt tgataaaatg gtttcgaaag
     2041 aaaactctct ggaagatgtt gtttctttag gtgaaatgat tcagaaggaa atttatgggc
     2101 atgaacaaag aacattttca ccagttcatc atacaggtaa ctggaaatca tcattgttac
     2161 acaacgcgct ccttggtctg gcaaatgttt ataatggctt acgggaaaca gaatacccta
     2221 acactttcaa cagagatggt ataaaaagta ctaactcttt tagagataac ttattgacaa
     2281 aaacaagaac tcccagagat aattttgagg aaggaataaa acatcctgaa catgcaacaa
     2341 taccatatga caacgacaat gaaagtaata aattgctaaa agcaggaaag atagctggta
     2401 acaataacga gctgttgatg gaaataaaaa aggaatccaa aagcgaccat caaatccccc
     2461 tgtcagataa gttcctgaaa aggaaaaaac ggtctcctgt agctgaagat aaagttcaaa
     2521 actcgttaac accagaaaat tttgttcaga aaatttcact tagtgatgag cttaaaacaa
     2581 aatatgcaaa tgaaattata gagataaaaa gaataatggg agaatacaat cttttaccgg
     2641 ataaaaacag tcgtaatggt ctaaaacttc tacagaagca agctgattta ctaaaaataa
     2701 tcatggagga tacctctgtt acagaaaata tctttaaaaa catagagatg gctatagcag
     2761 atataaaaaa ggagtactat tcgcatacag ttgatattga gaaaaatatt catgccatat
     2821 gggttgcggg ttccccacct gaaagcatct cggactatat taagactttt ctcaaaacct
     2881 acaaagagtt tacttactat ctttgggttg atgaaaaagc atttggagcc gcgaaattta
     2941 ccagtgtctt aaaacaaatt gcatttgatt tagcatgcag aactatacag cagaatactc
     3001 cacaaaaaaa tattgatttt attaatctat ataatgaaat aaggaagaaa tacaacaaca
     3061 acccatcggg acaacaagag tacttaaaca aactcagaga gctttatgct acttatcaaa
     3121 aaatatctac ccctctgaaa cacatgttta attcattttt tc
//
LOCUS       DAZFWL020000274         3146 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFWL020000274 DAZFWL020000000
VERSION     DAZFWL020000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.276"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            32..1237
                     /gene="tet(B)"
                     /locus_tag="ACHKNP_RS25205"
                     /old_locus_tag="pgaptmp_005041"
     CDS             32..1237
                     /gene="tet(B)"
                     /locus_tag="ACHKNP_RS25205"
                     /old_locus_tag="pgaptmp_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HGU4707012.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1350..1943)
                     /gene="tetC"
                     /locus_tag="ACHKNP_RS25210"
                     /old_locus_tag="pgaptmp_005042"
     CDS             complement(1350..1943)
                     /gene="tetC"
                     /locus_tag="ACHKNP_RS25210"
                     /old_locus_tag="pgaptmp_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HGU4707013.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2031..2447
                     /locus_tag="ACHKNP_RS25215"
                     /old_locus_tag="pgaptmp_005043"
     CDS             2031..2447
                     /locus_tag="ACHKNP_RS25215"
                     /old_locus_tag="pgaptmp_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4707014.1"
                     /translation="MYIEQHSRYQNKANNIQLRYDDKQFHTTVIKDVLLWIEHNLDQS
                     LLLDDVANKAGYTKWYFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALK
                     YQFDSQQSFTRRFKYIFKVTPSYYRRNKLWELEAMH"
     gene            complement(2457..>3146)
                     /locus_tag="ACHKNP_RS25220"
                     /old_locus_tag="pgaptmp_005044"
     CDS             complement(2457..>3146)
                     /locus_tag="ACHKNP_RS25220"
                     /old_locus_tag="pgaptmp_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HGU4707015.1"
                     /translation="KLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYK
                     RLTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEI
                     RTPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCW
                     LAGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDSLVAATLLAQN
                     LFTHGYALGKL"
BASE COUNT          833 a          576 c          679 g         1058 t
ORIGIN      
        1 ccactcacta tcagtgatag agaaaagtga aatgaatagt tcgacaaaga tcgcattggt
       61 aattacgtta ctcgatgcca tggggattgg ccttatcatg ccagtcttgc caacgttatt
      121 acgtgaattt attgcttcgg aagatatcgc taaccacttt ggcgtattgc ttgcacttta
      181 tgcgttaatg caggttatct ttgctccttg gcttggaaaa atgtctgacc gatttggtcg
      241 gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg gattacttat tgctggcttt
      301 ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt tcagggatca caggagctac
      361 tggggctgtc gcggcatcgg tcattgccga taccacctca gcttctcaac gcgtgaagtg
      421 gttcggttgg ttaggggcaa gttttgggct tggtttaata gcggggccta ttattggtgg
      481 ttttgcagga gagatttcac cgcatagtcc cttttttatc gctgcgttgc taaatattgt
      541 cactttcctt gtggttatgt tttggttccg tgaaaccaaa aatacacgtg ataatacaga
      601 taccgaagta ggggttgaga cgcaatcgaa ttcggtatac atcactttat ttaaaacgat
      661 gcccattttg ttgattattt atttttcagc gcaattgata ggccaaattc ccgcaacggt
      721 gtgggtgcta tttaccgaaa atcgttttgg atggaatagc atgatggttg gcttttcatt
      781 agcgggtctt ggtcttttac actcagtatt ccaagccttt gtggcaggaa gaatagccac
      841 taaatggggc gaaaaaacgg cagtactgct cggatttatt gcagatagta gtgcatttgc
      901 ctttttagcg tttatatctg aaggttggtt agttttccct gttttaattt tattggctgg
      961 tggtgggatc gctttacctg cattacaggg agtgatgtct atccaaacaa agagtcatca
     1021 gcaaggtgct ttacagggat tattggtgag ccttaccaat gcaaccggtg ttattggccc
     1081 attactgttt gctgttattt ataatcattc actaccaatt tgggatggct ggatttggat
     1141 tattggttta gcgttttact gtattattat cctgctatcg atgaccttca tgttaacccc
     1201 tcaagctcag gggagtaaac aggagacaag tgcttagtta tttcgtcacc aaatgatgtt
     1261 attccgcgaa atataatgac cctcttgata acccaagagg gcatttttta cgataaagaa
     1321 gatttagctt caaataaaac ctatctattt tatttatctt tcaagctcaa taaaaagccg
     1381 cggtaaatag caataaattg gcctttttta tcggcaagct cttttaggtt tttcgcatgt
     1441 attgcgatat gcataaacca gccattgagt aagtttttaa gcacatcatc atcataagct
     1501 ttaagttggt tctcttggat caatttgctg acaatggcgt ttaccttacc agtaatgtat
     1561 tcaaggctaa ttttttcaag ttcattccaa ccaatgatag gcatcacttc ttggataggg
     1621 ataaggtttt tattattatc aataatataa tcaagataat gttcaaatat actttctaag
     1681 gcagaccaac catttgttaa atcagttttt gttgtgatgt aggcatcaat cataattaat
     1741 tgctgcttat aacaggcact gagtaattgt tttttatttt taaagtgatg ataaaaggca
     1801 cctttggtca ccaacgcttt tcccgagatc tcatctattg aaacagcttg atagcctttt
     1861 tcaacaaaca atattcgtgc tgagttaacc agtgattgat aggtactctt aaaattttct
     1921 tgttgatgat ttttattttc catgatagat ttaaaataac ataccgtcag tatgtttatg
     1981 gtatcatgat gatgtggtcg tgacaatctt aagaacattt aggttatttt atgtatattg
     2041 aacagcattc tcgctatcaa aataaagcta ataacatcca attaagatat gatgataagc
     2101 agtttcatac aacggttatc aaagatgttc tattatggat tgaacataat ttagatcagt
     2161 ctttactgct tgatgatgtg gcgaataaag cgggttatac caagtggtat tttcagcggc
     2221 tgttcaaaaa agtaacaggg gtcacactgg ctagctatat tcgtgctcgt cgtttgacga
     2281 aagcggctgt tgagttgagg ttgacgaaaa aaactatcct tgagatcgca ttaaaatatc
     2341 aatttgattc ccaacaatct tttacacgtc gatttaagta catttttaag gttacaccaa
     2401 gttattatcg gcgtaataaa ttatgggaat tggaggcaat gcactgagag atcccctcat
     2461 aatttcccca aagcgtaacc atgtgtgaat aaattttgag ctagtagggt tgcagccacg
     2521 agtgagtctt cccttgttat tgtgtagcca gaatgccgca aaacttccat gcctaagcga
     2581 actgttgaga gtacgtttcg atttctgact gtgttagcct ggaagtgctt gtcccaacct
     2641 tgtttctgag catgaacgcc cgcaagccaa catgttagtt gaagcatcag ggcgattagc
     2701 agcatgatat caaaacgctc tgagctgctc gttcggctat ggcgtaggcc tagtccgtag
     2761 gcaggacttt tcaagtctcg gaaggtttct tcaatctgca ttcgcttcga atagatatta
     2821 acaagttgtt tgggtgttcg aatttcaaca ggtaagttag ttgctagaac ccatggctcc
     2881 tttgccgacg ctgagtagat tttaggtgac gggtggtgac aatgagtccg tgtcgagcgc
     2941 tgattttttc ggcctttaga gcgagattta tacaatagaa tttggcatga gattggattg
     3001 cttttagtca gcctcttata gcctaaagtc tttgagtgac tagatgacat atcatgtaag
     3061 ttgctgatag gtttccagtt ttccgctcct aggtctgcat attgtacttt tcctcttact
     3121 cgacttaacc agtaccaacc cagctt
//
LOCUS       DAZFWL020000275         3130 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFWL020000275 DAZFWL020000000
VERSION     DAZFWL020000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.277"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            52..2412
                     /locus_tag="ACHKNP_RS25225"
                     /old_locus_tag="pgaptmp_005045"
     CDS             52..2412
                     /locus_tag="ACHKNP_RS25225"
                     /old_locus_tag="pgaptmp_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGU4707016.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(2574..2750)
                     /locus_tag="ACHKNP_RS25230"
                     /old_locus_tag="pgaptmp_005046"
     CDS             complement(2574..2750)
                     /locus_tag="ACHKNP_RS25230"
                     /old_locus_tag="pgaptmp_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707017.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(2888..3130)
                     /locus_tag="ACHKNP_RS25235"
                     /old_locus_tag="pgaptmp_005047"
                     /pseudo
     CDS             complement(2888..3130)
                     /locus_tag="ACHKNP_RS25235"
                     /old_locus_tag="pgaptmp_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          780 a          667 c          810 g          873 t
ORIGIN      
        1 gacccgaatt tctgccgtgg tgcctcagtg gatcggatac aggtaacgaa tatggttcat
       61 aaatctgaca gtgatgaatt agctgcattg agggcagaaa atgcccgtct ggtctcatta
      121 cttgaagctc atgggattga atggcgacgt aaaccgcaga ctcctgtgca gcgcgtttct
      181 gtattatcca ccgacgagaa ggttgcatta tttcgtcggt tgtttcgtgg gcgtgatgat
      241 gtatgggcac ttagatggga aagtaaaacc agcggcaaat cagggtactc tcctgcctgc
      301 gctaacgaat ggcaggcgag aatatgcggt aaaccccgga taaaatgtgg ggactgcgct
      361 caccgtcagt tgattcctgt atccgatctc gtcatctacc accatctggc cggtacccat
      421 actgtcggga tgtatccgtt gctggaagat gattcctgtt attttctggc agttgatttt
      481 gatgaagctg aatggcagaa ggatgcatcc gcattcatgc gatcctgtga tgagctgggt
      541 gtacctgctg cgctggaaat atcccgttca cgtcaggggg cgcatgtctg gatatttttt
      601 gcctcacgag tttcggcccg cgaagctcgc cgtctgggga ctgctattat tagctatacg
      661 tgtagtcgga cccgacagct gcgattgggg tcttatgacc gattatttcc taatcaggat
      721 actatgccaa aagggggatt tggtaatctc atagcgttac ctctgcaaaa aagaccgcgt
      781 gaattggggg gaagcgtttt tgttgatatg aatctccagc cttatcctga tcagtgggct
      841 tttcttgtat cggtgacccc gatgaatgtg caggatattg aaccgacgat attacgggct
      901 acagggagta tccatcctct ggatgtgaat tttatcaacg aagaagacct gggtacgccg
      961 tgggaagaga aaaaatcatc aggaaacaga ctgaatattt ctattgcaga accgctgaaa
     1021 atcacgctgg caaaccagat ctatttcgaa aaagcgcaat tacctcaggt gctgattaac
     1081 cgacttattc ggctggcagc atttccgaac cctgagtttt ataaggctca ggcaatgcgt
     1141 atgtcagtct ggaataagcc ccgtgttata ggttgtgcgg agaattaccc gcaacacatt
     1201 gcgttgcccc ggggatgtct ggacagcgta ttatctttcc ttagggacaa caatattgct
     1261 gcagaattaa tcgataaacg atttgccggg acggaatgta atgccgtttt tatgggaaac
     1321 ctcagagcgg agcaggaaga ggccgtttcg gcattactcc gttatgacac tggtgtgctt
     1381 tgtgcgccaa cggcttttgg taagacagtt accgcagcgg cagtgattgc caagcggaaa
     1441 gtgaatacac tgatactggt acaccggact gaattgctga agcagtggca ggagcgtctc
     1501 gcggtgtttc tgcaggccgg tgacagtatt ggtattatcg ggggaggtaa acataaaccc
     1561 tgtggcaata ttgatattgc ggtggtgcag tccatatcca gacaaggaga agttgaacct
     1621 ctggtcagga attatgggca aatcattgtg gatgagtgcc atcatattgg cgcggtttca
     1681 ttttctgcga ttctgaagga aacgaatgcc agatatctgc ttggcctgac ggcaacacca
     1741 atccgacggg atggtctgca tcccattatt tttatgtact gtggtgccat tcgccataca
     1801 gcggtccgcc cgaaggaaag cccacataat ctggaggtac tgatccgttc ccgttttaca
     1861 tctggtcatt taccatcgga tgcgagaatc caggatattt tcagagaaat tgctctggat
     1921 catgacagaa cggtggcgat agctgaagaa gccatgaaag ctttcgggca ggggcgaaaa
     1981 gttctggtac tgactgaacg tacagatcat ctggatgaga tagcatcagt gatgaattca
     2041 ctgaaattgt ctccctttat tctccatggt cgactatcga aaaaaaagcg tgcgatgctg
     2101 atatccgggc tgaatgctct tcctcccgat tctcctcgaa ttttgttgtc aacaggcaga
     2161 cttattggtg agggatttga ccaccctccg ctggatacgc tgattcttgc catgcctgtg
     2221 tcatggaaag ggacattaca gcagtatgca gggcgtcttc acagagagca taccggtaaa
     2281 agcgatgtca ggatcattga ttttgtggat accgcgtatc ctgtgctgct cagaatgtgg
     2341 gataaacgtc agcggggtta taaagcgatg gggtacagga ttatagctga cggcgatgaa
     2401 tcagtgattt aattttaagg agtactttac cctttttaaa taatttgagc tccccgggtt
     2461 gttcgggcaa caaagagtcc cagtatacag gcttcaggaa tatgttgtag tatcaggctt
     2521 ttcatcgctt taaaatgact accggcagtg agaacgtcat caaaaataac aatctagggc
     2581 ctgcaccctt ccagtaattg ttcatccagt tggtagtttg ccataatcgc ttctggtaag
     2641 ggccggtttg ttgcggtatg agaggctatt gttgatacgt tttgcacaac cagttcccag
     2701 aaatccagcc atggttcgta ggtttgcgca attcgcagaa tttctgtcat acggtcatcg
     2761 taagcggcat ctgtacggca tttggatggt ggtacgggaa caaatgtgta ttcctcaaga
     2821 ttaccgatat ttgatatgag catctgagca acatactcaa tagcagcctt tttatagtgg
     2881 taatcaggct acgaaagtac agcgttttat aaattgtctc aggtgatatt cgcagcgttt
     2941 tttgacgtgg ttttgttcgc cttaaccatc ctgatatttg ctctggagac catttcatct
     3001 ccagcttttc cagaacaagc tttcgcaatg gtaaattttg atccagtaag cacggttttg
     3061 gccttttcgc cattctgttg gctcggttat tagcatcaac agctttgtaa tagcgtctgc
     3121 cccgattacg
//
LOCUS       DAZFWL020000276         3069 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFWL020000276 DAZFWL020000000
VERSION     DAZFWL020000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.278"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            55..1431
                     /gene="ygeH"
                     /locus_tag="ACHKNP_RS25240"
                     /old_locus_tag="pgaptmp_005048"
     CDS             55..1431
                     /gene="ygeH"
                     /locus_tag="ACHKNP_RS25240"
                     /old_locus_tag="pgaptmp_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HGU4707018.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTPESIYR
                     AMTIFDILQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHPTDIASLYYYRALVHFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLK
                     QLTRICMR"
     gene            1599..1817
                     /gene="ygeI"
                     /locus_tag="ACHKNP_RS25245"
                     /old_locus_tag="pgaptmp_005049"
     CDS             1599..1817
                     /gene="ygeI"
                     /locus_tag="ACHKNP_RS25245"
                     /old_locus_tag="pgaptmp_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HGU4707019.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
     gene            1900..2402
                     /locus_tag="ACHKNP_RS25250"
                     /old_locus_tag="pgaptmp_005050"
                     /pseudo
     CDS             1900..2402
                     /locus_tag="ACHKNP_RS25250"
                     /old_locus_tag="pgaptmp_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(2421..3053)
                     /locus_tag="ACHKNP_RS25255"
                     /old_locus_tag="pgaptmp_005051"
                     /pseudo
     CDS             complement(2421..3053)
                     /locus_tag="ACHKNP_RS25255"
                     /old_locus_tag="pgaptmp_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
BASE COUNT         1082 a          550 c          497 g          940 t
ORIGIN      
        1 tttctattgc ttaagcagga tgcaagaaac caattttttc atagaggtta actaatggac
       61 ttagaaaata aattctcata tcattttctt gagggattaa cgctcacgga agatggaatt
      121 cttactcaag gaaatgagca agtttatatt ccacagaaag agttaggtgt attaatagta
      181 ttacttgaat ccgctggtca tgttgtactg aaagatatga tcatcgaatc agtatggaaa
      241 aatattattg ttagtgacga gtccctgaca agatgtatct attctttgcg ctgcattttt
      301 gaaaaaattg gctatgatcg ttgcatagaa acaatctacc ggaaaggtta tcgtttcagc
      361 gggcaggttt tcaaaactaa aattaatgaa gataatactt cagactattc catagctata
      421 ttccctttca ctacttcgtt gaatacactg gatccattaa tacttaatca ggaattagtg
      481 caaatcattt caaataaaaa aatcgatggt ctctatacct atccgatggc tgcgacaaat
      541 ttttgtaatg atcacatatc tcaaaattca ttcttgagca gattcaagcc agattatttc
      601 gttacaggaa gaataaacca gaataatgca gtgaacactt tatacattga gttgatcgac
      661 gctaaaaacc ttttcctcat cgccagtaat catctccctg ttgatgaact acataataca
      721 tcacaattta ttatagataa tatccttcaa acggtacata aaccagaacg atctgtaaga
      781 ttagctaagc aggaccaagg atataagaat cattatttat cagatgaaat gttagccgga
      841 aagaaagaac tttacgactt cacccctgaa agcatttaca gggccatgac tatatttgat
      901 atattacaaa ataaaagtga tatacagacg ctaaaaacag aatgttattg ccttctagcg
      961 gaatgccata tgtctttggc acttcatgga aaaagtgaac ttgaacttgc tgctcaaaaa
     1021 gcattagagc ttttagatta tgtatcagac ataaccactg tcgatggaaa aattttagct
     1081 attatgggac tgataactgg tctgtctgga caagcaaaag tatctcatat cttatttgaa
     1141 caggctaaga tacacccaac tgatatagcc tctctctact actatagggc actagtccac
     1201 tttcataatg aaaaaattga agaggcaagg atttgtatag acaaatcact acaactcgaa
     1261 cccagaagac gaaaagcagt tgtgataaaa gaatgtgtag atatgtatgt gcctaacccg
     1321 ctcaaaaaca acattaaact ctactataaa gaaactgaga gtgaaagcca tcgagttata
     1381 attgacaaca ttttgaaatt aaagcagctg acgagaattt gtatgcgata aatttttatt
     1441 gacgcaattt tattatttag atgtatgcaa cttgttattt aaacaacaga gataaaaata
     1501 tctaagatat tcaccttatt gcaagatatt taaaatgctc tagagttaaa agtatgatgt
     1561 aaagaccatt ggcctggaca attaaaaaag ggagctttat gactaatcca atcggtatca
     1621 ataatttatc tcaaagttca aatatagcga atgcaacggg tgatgaagtc gtatcattag
     1681 ataaacacat caacaccagt gctactgata cagatcaaat acaagcattt atagtttcaa
     1741 catggatggc gccttttcaa aatgatatgt attcagaaga taatcctatc tcaccttact
     1801 ataaaattga atggtagacg attgaccaaa taaatcataa cgagatactt ttataatagt
     1861 atctcgtccc tttcattcaa tcctcacaca tgaaaaaata tgcgctcacc aaaagttaaa
     1921 ttcttaacca tcttcacgtt ttgtatattt attacaaaaa tgtctttcgc cagcaactct
     1981 tgctctaatg aagcaggaac aatgttcaga atcgaaccaa atttaattaa agctatcgct
     2041 cttgttgaaa gcaatctaaa aaaggatagc ataggcaaga acagagataa gaataacaat
     2101 ataaagagtt tagattattg gttaatgcag ataaaccaga tgcatattcc cctgttaaaa
     2161 aaacgtggaa taataaaaga tgaacgagat ttgttagata acccttgtct gaacattaaa
     2221 ataggaaccg agattttata taaccatttt tcacgttgtg gagtaacgtg gcaatgcctg
     2281 gggacttata atgcgggttt tgcaatggat aaccaaaaaa agagacagca atatgcccca
     2341 aaatatacat tgtatatacc aggcttaatg aactagacaa tcgtaaggct tcagcaaaat
     2401 gagcctaaag cctctttttt ttatatagtg caaacaccca tacgtaaagc tgttttgatc
     2461 aactcgatac cactgtgaac atctaatttc ttcatgatat tgagtctatg agtttcgact
     2521 gtttttctgc ttaactgcaa gaaattagcg atttctttgt ttgttttacc atcagctatg
     2581 agttgtaata tttctgactc acgatttgta agttgattat cagaggaata cctggaagat
     2641 attttttcac aatccatatg tacactatca aaataagtgt aaccattact aatcgaatca
     2701 atagcacgat ttagtttcgc cgggtgactg gttttccata cgcatccttt cgcacctgct
     2761 tcaagcaatt ttattgaatc aatatacggc atctttgcta caaatgtaat aatatgagca
     2821 tctatcttat gcgatttaac ccattttacg agttcggacc ccatcattcc acctgcaaat
     2881 tctcctaaaa aaaatattta ggctgtagat ttgttacact ccgctatagc tttcttcaga
     2941 tctttatagc ccccaacaac ctcatataaa tcaggataat gtccgagaaa tccaattatc
     3001 ccatcaatca taaacggctg ctgatctgaa actacaattt taatttttcc catatattca
     3061 tcctgagtg
//
LOCUS       DAZFWL020000277         3029 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFWL020000277 DAZFWL020000000
VERSION     DAZFWL020000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.279"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..549
                     /gene="escR"
                     /locus_tag="ACHKNP_RS25260"
                     /old_locus_tag="pgaptmp_005052"
     CDS             <1..549
                     /gene="escR"
                     /locus_tag="ACHKNP_RS25260"
                     /old_locus_tag="pgaptmp_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HGU4707020.1"
                     /translation="KISIVLGILKNALGIQQVPPNMALTSVSLILTMFIMSPIILQIN
                     DNISQEPINYTDSDFFQKVDEKILSPYRGFLEKNTEKENVEFFERAAQKKLGNETILK
                     KDSLFILLPAFTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTISIPF
                     KILLFILVGGWQKLFEFLLVVN"
     gene            549..818
                     /gene="escS"
                     /locus_tag="ACHKNP_RS25265"
                     /old_locus_tag="pgaptmp_005053"
     CDS             549..818
                     /gene="escS"
                     /locus_tag="ACHKNP_RS25265"
                     /old_locus_tag="pgaptmp_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HGU4707021.1"
                     /translation="METGYFVQLCVQTLWIIFILSLPTVIAASVVGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            818..1594
                     /gene="escT"
                     /locus_tag="ACHKNP_RS25270"
                     /old_locus_tag="pgaptmp_005054"
     CDS             818..1594
                     /gene="escT"
                     /locus_tag="ACHKNP_RS25270"
                     /old_locus_tag="pgaptmp_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HGU4707022.1"
                     /translation="MNEIMTVIVSSFFCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIVLKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFITVIFVIHGGIQSVLDKLYLSYEILPLQAD
                     IAFNRALVDFLFSLWDSFIKLMLSFSIPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLP
                     VKSLIAIFILLLVIHVFPDFITANIHSDIIDRSLPSMINE"
     gene            1587..2624
                     /gene="escU"
                     /locus_tag="ACHKNP_RS25275"
                     /old_locus_tag="pgaptmp_005055"
     CDS             1587..2624
                     /gene="escU"
                     /locus_tag="ACHKNP_RS25275"
                     /old_locus_tag="pgaptmp_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HGU4707023.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVTKSEEVMAAVQSLILFSFFSLY
                     GASFFVEVVELVNTTIDSLNRPFLYAIREILGAVLNIFLLYILPISLIVFVGTVTTGV
                     SQTGFIFAVEKIKPSAQKISVKNNLKNIFSVKSIFELLKSVFKLVIITLIFYFMGHSY
                     ANEFANFTRLNAYQTLVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEGLKKMKMSKD
                     EVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHIAICLYYKLGE
                     TPLPLVIETGKDAKALQIIKLAELYDIPVIEDITLARSLYKNIHKGQYITEDFFEPVA
                     QLIRIAIDLDY"
     gene            complement(2621..>3029)
                     /gene="etgA"
                     /locus_tag="ACHKNP_RS25280"
                     /old_locus_tag="pgaptmp_005056"
     CDS             complement(2621..>3029)
                     /gene="etgA"
                     /locus_tag="ACHKNP_RS25280"
                     /old_locus_tag="pgaptmp_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HGU4707024.1"
                     /translation="SDCFEINGKAYNIDPLILKAIAWNESKNKNGIKSKINKNGTYDI
                     GIMQINSSHLDLLSKFNISEDDLLNDACINISVAGYILASNIKSRGNTWDAVGAYNAG
                     YFNTPNAVELRRQYAMKIYKTYTKLKNNEQIID"
BASE COUNT          920 a          482 c          535 g         1092 t
ORIGIN      
        1 aaaatttcga ttgtattggg gattctgaag aacgcattag gtattcaaca ggtaccacca
       61 aatatggcgc taacatcagt atctttgata ctgacaatgt ttattatgtc tccgataata
      121 ttacagataa atgataatat ttctcaggaa ccaatcaatt acaccgactc tgattttttt
      181 caaaaagttg atgaaaaaat attatcacca taccgcggtt ttttagaaaa aaatacagag
      241 aaagaaaatg ttgagttttt tgaacgtgca gctcaaaaaa aattgggtaa tgaaactatc
      301 ttaaaaaaag actctctatt tatactgtta ccagctttca cgatggggca gcttgaagct
      361 gcattcaaga taggtttttt gctctattta ccttttattg cgatagattt gatcatttcc
      421 aatatcttat tggccttggg tatgatgatg gtatcgccag taacaatttc gattcctttt
      481 aagatcttgt tattcattct tgttggaggc tggcaaaaat tattcgaatt tctgttggtg
      541 gtgaattgat ggaaactgga tattttgttc aattatgtgt gcaaacgctt tggataatat
      601 ttatcctctc actgcctaca gtcatagcgg cctctgttgt cggcattatt attagcctgg
      661 ttcaggctat aacgcagtta caggatcaaa cattgccttt tttgctaaaa ataatagcag
      721 tgtttgctac gcttgccctg acttatcact ggatgggaac aacaatcatc aacttctcgt
      781 cgataatatt tgaaatgatt ccgaaggtga atggctaatg aatgaaataa tgacggtcat
      841 agtatcatca tttttttgta tactcaggcc gctggggatg tttattattc taccaatatt
      901 ttcaacaggt gttttgctca gtaactttat acgcaactca attatgattg cgtttacact
      961 tccaattata gtagagaact atacattttc ggaaaaatta ccatccggaa tttttcagtt
     1021 gacaggcata gtgctaaaag aaataagtat aggctttttc attgggttat catttactat
     1081 tcttttttgg gcaatagatg cggctgggca gattattgat actttaagag gttcaacaat
     1141 atcttcaatt tttaacccgt ctataagtga ttcatcttct atcactggtg ttattttata
     1201 ccaatttatc actgtgatct ttgttattca tggtgggata caaagcgttc tggataagtt
     1261 gtatttatcc tacgagatat taccattaca agccgatatt gcattcaatc gtgctttagt
     1321 agatttttta ttttcgctat gggattcatt tattaaactg atgttatcat tttcaattcc
     1381 catgattatc ggtatcttct tatgtgatat ggggtttggg tttctcaata aaacagcgcc
     1441 tcagctaaac gtattcacat tatcactgcc ggtaaaaagt ttgatagcaa tctttatatt
     1501 gttattagtt attcatgttt tccctgattt tattacggca aatattcatt ctgacataat
     1561 tgacagatcg ttaccgagca tgattaatga gtgaaaaaac agaaaagccc acacccaaaa
     1621 aactaaggga tctaaaaaag aagggcgatg taacaaaaag tgaagaggta atggctgcag
     1681 tgcagtcatt aatcttattt tcattttttt ctttatatgg tgcgagtttt ttcgttgagg
     1741 ttgttgagtt agttaatacg actatagact cgctaaatag accgtttttg tatgccattc
     1801 gtgaaatatt aggtgcggta ttaaacatat ttttattgta tattttgcca atttctttga
     1861 ttgtctttgt tggaactgtt acaactggtg tatcacaaac aggattcatc tttgcggttg
     1921 aaaaaataaa accatcggct cagaagatta gtgtaaaaaa taacctgaaa aatatttttt
     1981 ctgtaaagag tatttttgag ctgcttaagt cagtatttaa gttagtgata attactctca
     2041 ttttttactt tatggggcat tcatatgcaa atgagtttgc aaatttcaca agactgaacg
     2101 catatcaaac tcttgtcgtt gttgcctttt ttgtttttct tttatggaaa ggcgtgttat
     2161 tcggatatct actcttttca gtatttgatt tctggttcca gaagcatgag ggactgaaga
     2221 aaatgaaaat gagtaaagat gaggtgaaac gagaagccaa ggatactgat ggtaaccctg
     2281 aaattaaagg ggagcgccgt cgccttcatt ctgagataca aagtggaagt ctggctaata
     2341 acatcaaaaa atcaaccgtg atcgtcaaaa acccgactca cattgcgatt tgcctatact
     2401 ataaactcgg ggagactcca ttgcctttag ttattgaaac aggaaaagat gccaaagctc
     2461 tacagatcat taaactggct gaactctatg atattccagt gattgaagat attactttgg
     2521 caagaagtct ttataagaac atacataaag gacaatacat aacagaagac ttttttgaac
     2581 ctgtagcaca attgattcgt atagcaatag atcttgatta ttaatcgata atttgttcat
     2641 tgttttttaa tttagtgtat gttttatata ttttcattgc atattgtctt ctgagttcta
     2701 ctgcatttgg tgtgttaaag tagcctgcat tatacgcacc aactgcatcc catgtattcc
     2761 ccctggattt tatgttagag gctaagatat aaccagcaac agatatgttt atacaggcat
     2821 cattcaataa atcatcttca gatatattaa atttgcttaa taaatctaaa tgagatgaat
     2881 ttatttgcat tatacctata tcatacgttc cgtttttgtt tattttactt tttattccgt
     2941 ttttgttttt tgattcgttc catgcaattg cttttaatat taatgggtct atattatatg
     3001 cttttccatt aatttcaaaa caatcagaa
//
LOCUS       DAZFWL020000278         3028 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFWL020000278 DAZFWL020000000
VERSION     DAZFWL020000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.280"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(27..1886)
                     /gene="gss"
                     /locus_tag="ACHKNP_RS25285"
                     /old_locus_tag="pgaptmp_005057"
     CDS             complement(27..1886)
                     /gene="gss"
                     /locus_tag="ACHKNP_RS25285"
                     /old_locus_tag="pgaptmp_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HGU4707025.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQEGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNINLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2091..2957
                     /gene="yghU"
                     /locus_tag="ACHKNP_RS25290"
                     /old_locus_tag="pgaptmp_005058"
     CDS             2091..2957
                     /gene="yghU"
                     /locus_tag="ACHKNP_RS25290"
                     /old_locus_tag="pgaptmp_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGU4707026.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT          711 a          815 c          768 g          734 t
ORIGIN      
        1 tcagtacggg gttgctacaa catgtattac tttttcacca caattaacgg ttcaatatca
       61 ctctcttttt tgatgaccag tgattcatca ccgcgcaaac acgtcccacc gtagttgccg
      121 ccaacggtga aggtacatac ctgaatgtat ttaccgtcca ctttcggcaa acaccacagt
      181 tgctgataga tgtttttctg ctcggcaaat ttaccgctgg ttttgtccag cacctcttca
      241 tgatggctga ccaggttgat attgctacca cagcgaccgg caatcggttt cactgcgtaa
      301 cctgttttca ccaattcatc attaacagtg aaatcggtat ccagcaggta acgatggtgc
      361 gggaacagcg accagaggat cggcagaatc gctttgttgc cggggatcac cgtccacagc
      421 ggctcaaaga ctagcacttc cgggcgcagc aatacgtcga taagacgcac ttcgttttgc
      481 ggatgaccgg tacggattgg caccgcagca aactcacggt cgctaacttc acgaatctga
      541 tcaaacgcgg tttcccacgc ccaggttttc cacacgcagt taaccagccg tccttcccca
      601 tcaatcagtt gcccggcagc atcccagccc agttcatcca gcccgcgcaa gatacgcgtt
      661 tcaaagccct cctggtgcag cgcctgctcc ataaactgcg cgtgatagtt ttcctcgata
      721 tctttgtcct gcatgatatg gacaaacgga cgtgcacgac tgtgtttcca ggcaccagcc
      781 agttcgttaa tcagcccttc cgccggattg aagccgttgc ctttatagcc ctgctccgcc
      841 caacgttcga ggatcaagcc cgcttcggta tgacatgagg cagagtcggc gttgtattcg
      901 taaaccttca ggccacgctc atccatgcag aaatccatac gaccagtgat catatggtga
      961 cggcgacgct gccaggagag acgcaaacgt ggccagagga ttttcgggat gtcgaacagc
     1021 gccagcaggt tgtcatcttt cagcaccttg tcggttgcgt gaagatacat caggtgcagc
     1081 tcgttggtgg ctttaattag ctcctgctcg gcactctcgg taatggtgta gtaatgataa
     1141 ggatcctgat tgatcacctg accgttggcc tgcacatagg cgttttgcag cggatctttt
     1201 tcatccagcc atttaccgtc aaactggcct ttgttttcca ggcgcgctcc gctgattttc
     1261 agcagctcgc ctgcaatttc cggctgcggt aagctgtatt cagtatcttc cgtctggatc
     1321 atccagccca gaatggtggt gtcatcaaaa gtgtctttca gggtatagca gccgttttcg
     1381 accaccatct ccagctcacg cgtccactgt tgcccttgcg gcaacgggga atgaatcacg
     1441 ttctgttccg caatacggac tttgttgcca tgcaattggg taatgatggc gacatggcca
     1501 gtgtctttaa attcaccgcc tttatcccag ataagaagcg cacccgcgac cggcgcacgc
     1561 ggcgagccgt taggaaatgc ctgcaatggc aggatgttgt cattaaccac ttctcgcagg
     1621 aagcgcagcg agaaaatctc ccacgccata cccacgtcag taaagaccac accgtaattc
     1681 agaaagagaa aacggcgagc aaattcaacg cattgccact tgtggcccat atattcgtcg
     1741 tcgatatagc tacggaatac ggcgtcatct tcgtattcct gcggatcgag agaactgtaa
     1801 tctgaagagt agattgctac cccacctggg gcgtagccca ataatgtccc gaacggggca
     1861 tcctggctgg tcgttccttt gctcatcact ttacctttaa caatacaacc taagcagttg
     1921 gcgtaattgt tgtgctctga ttacctgctc agcgacatta accggacaga ggtcagacta
     1981 atcatacacc tcatcgcagc ggctggcgca aagggttaaa aaattcacat tctgtacagc
     2041 aagtgacctg ctacactgct tcaacactac cactcagaag gcaactcact atgacagaca
     2101 atacttatca gcccgcgaaa gtctggacgt gggataaatc cgctggcggc gcgttcgcca
     2161 atatcaatcg cccggtttct ggtccgacgc atgaaaaaac gctgcccgtt ggcaaacacc
     2221 cattgcaact ttattcgctg ggaacgccga acggtcagaa agtaacgatt atgcttgagg
     2281 agctgctggc gctgggcgtt actggtgcag agtacgacgc ctggctgatt cgtattggcg
     2341 atggcgatca attctccagc ggctttgtcg aagtgaaccc aaactcgaag atcccggcgc
     2401 tgcgcgatca tacgcataat ccgccgatcc gcgtgtttga atctggttcg atcctgcttt
     2461 atctggcgga gaaatttggc tacttcctgc cgcaggattt ggcaaagcgt actgaaacga
     2521 tgaactggct gttctggtta cagggcgcgg caccgttcct cggcggtggt tttggtcact
     2581 tttaccatta cgcgccgatg aaaattgagt acgccatcaa ccgctttacc atggaagcca
     2641 aacgtctgct cgacgtgctg gataagcaac tggcgcagca taagtttatt gcgggcgatg
     2701 agtacaccat tgcggatatg gcgatttggc cgtggtttgg caatgtggtg ttaggcggcg
     2761 tgtatgatgc cgcagagttt cttgatgcgg gcagttataa gcatgtacaa cgctgggcga
     2821 aagaagtagg cgaacgtccg gcggtgaaac gtgggcgtat tgttaaccgc accaacggac
     2881 cgctgaatga gcagttgcat gagcgccatg acgccagtga tttcgagacg aatacggaag
     2941 ataagcgtca ggggtaaggg ttggtgttcg tcgcagcaag ccatccaggc cggataaggc
     3001 gttcacgccg catccggcaa tcgtgcac
//
LOCUS       DAZFWL020000279         2993 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFWL020000279 DAZFWL020000000
VERSION     DAZFWL020000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.281"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(116..2377)
                     /gene="katE"
                     /locus_tag="ACHKNP_RS25295"
                     /old_locus_tag="pgaptmp_005059"
     CDS             complement(116..2377)
                     /gene="katE"
                     /locus_tag="ACHKNP_RS25295"
                     /old_locus_tag="pgaptmp_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HGU4707027.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            2560..2823
                     /gene="cedA"
                     /locus_tag="ACHKNP_RS25300"
                     /old_locus_tag="pgaptmp_005060"
     CDS             2560..2823
                     /gene="cedA"
                     /locus_tag="ACHKNP_RS25300"
                     /old_locus_tag="pgaptmp_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HGU4707028.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT          719 a          724 c          768 g          782 t
ORIGIN      
        1 caatacattg aatttatacg actttgtagg ccggataagg cgttcacgcc gcatccggca
       61 taaacaaagc gcactatgta aatcatttga ggcggcgcaa ttgcgccgcc tcccatcagg
      121 caggaatttt gtcaatctta ggaatgcgtg accacacgcg gtgtgctgcc atcagcgtta
      181 gcagttcatc cataaaacta ccgtcagcgc tgtcagcttc cacaatccct tcttcaccct
      241 ggtcagcgac cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt
      301 taaggtgttt gtaggcttcc atcaggtagt agttggcatc gccgttgtca gcgatatccg
      361 cgatattgcc gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg
      421 tagcggctat aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga
      481 gcagtttggc atgaacgcct ttggccttca gggccttgag aatggccaga aggtctgccg
      541 atctcacttc atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa
      601 tggcgtacaa acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga
      661 tattcagctg gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag
      721 tgagatcaat atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt
      781 tgcttaactc aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta
      841 gccagaacag acgcggatgg gaataatatt cgccaaacga tggactgcgc tcacgaactt
      901 tatttccttc cacgcgttcc tgatatgatt caaaaccgcc gcgtttcggt cccggcggtg
      961 tttcgcgcgg ccagttatcg ttgatcgagt tcggttcgta attcgccggg ttagtgtcga
     1021 tccccatacg atgcatgcca tcacgctgga agttgtggta agggcaggtc ggacggttaa
     1081 tcggaatctc atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga
     1141 acaaacgtcc ctgcaacagc ggatcgttgg tgaagtccag accaggaaca atatgacctg
     1201 gatggaatgc tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca
     1261 ttttgccgac acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa
     1321 gatcgaagtc gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt
     1381 ccggaaaatc gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac
     1441 gtccggtgag tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc
     1501 agtggaaacg tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac
     1561 cgaagccttc catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca
     1621 cgttgtgcag agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt
     1681 gcccttgtgg aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt
     1741 tatgcgcatc ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct
     1801 cttcagtata gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag
     1861 cgccaccctg aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga
     1921 ggaaatccgc tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg
     1981 ctgatccgcg tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt
     2041 tctcgcgcag aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt
     2101 tttgatcatc ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt
     2161 tgcgtacgtc ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc
     2221 caggggcggt aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt
     2281 cctcaggtgc cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact
     2341 ggtgctgatg tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt
     2401 actgaaaggg ccgctgtttt ggtcaaaaaa ccctctaact atagaccatc agccagatcc
     2461 cgcttcggag actattctgg aaacggtgtt atgaaataca cgcaaagtgg gacgtacagg
     2521 ggctaaatcg gctaaactac agtttttctg atttttaata tgcgtttagt gaagccagta
     2581 atgaagaaac cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa
     2641 cccgcgccgc cagaacatgc gataaagatg gattcttttc gtgatgtctg gatgctgcgt
     2701 ggcaaatatg ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact
     2761 gtgcctgaat ccgcccagcg ttgggcaaat cagatccgcc aggaagggga agtgactgag
     2821 taaagattac gtacctcccg gttttgccgg gagttacatg acctgaaatt ctgtatttca
     2881 ttgctacatt cattcaaacc accagtaaca aaaagctgcc tttgacgatc tattgctata
     2941 aataagtgat cttttttctt taattgttta tttattttaa aatggttaag ttt
//
LOCUS       DAZFWL020000280         2943 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFWL020000280 DAZFWL020000000
VERSION     DAZFWL020000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.283"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            34..792
                     /gene="paa"
                     /locus_tag="ACHKNP_RS25305"
                     /old_locus_tag="pgaptmp_005061"
     CDS             34..792
                     /gene="paa"
                     /locus_tag="ACHKNP_RS25305"
                     /old_locus_tag="pgaptmp_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HGU4707029.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            1128..1283
                     /gene="hokD"
                     /locus_tag="ACHKNP_RS25310"
                     /old_locus_tag="pgaptmp_005062"
     CDS             1128..1283
                     /gene="hokD"
                     /locus_tag="ACHKNP_RS25310"
                     /old_locus_tag="pgaptmp_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU4707030.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1504..1761
                     /locus_tag="ACHKNP_RS25315"
                     /old_locus_tag="pgaptmp_005063"
     CDS             1504..1761
                     /locus_tag="ACHKNP_RS25315"
                     /old_locus_tag="pgaptmp_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707031.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1831..2109
                     /locus_tag="ACHKNP_RS25320"
                     /old_locus_tag="pgaptmp_005064"
     CDS             1831..2109
                     /locus_tag="ACHKNP_RS25320"
                     /old_locus_tag="pgaptmp_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707032.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            2111..>2943
                     /locus_tag="ACHKNP_RS25325"
                     /old_locus_tag="pgaptmp_005065"
     CDS             2111..>2943
                     /locus_tag="ACHKNP_RS25325"
                     /old_locus_tag="pgaptmp_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4707033.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     T"
BASE COUNT          780 a          693 c          775 g          695 t
ORIGIN      
        1 ttatttcatg ataaccaata aaaggagttt tttatgagga acataatggc aggtttttta
       61 atattcctgt cttctgctgc ttatgctgat atcaatctgt atggtcctgg tggcccgcat
      121 acagccttgc ttgatgcagc caaactttat gccgaaaaaa caggtattat agtgaacgtt
      181 cattacggcc cacagaacaa atggaatgaa gatgccaaaa aaaatgcaga tatcttgttt
      241 ggcgcatcag aacaatctgc tctggctatc attcgggacc ataaagacag cttcagtgaa
      301 aaagatattc agcctcttta tctgcgaaaa agtattttac tggtaaagaa aggtaatcct
      361 aaaaatatcc ggagtattga cgacctgacc agacctggga ttggcgtaat tgttaatgat
      421 ggtggtggta ccagtaatac atcaggcact ggcgtctggg aagatattgc cggacgtaaa
      481 gggaatatag aaactgtcgc cgcaatccga aaaaatatta ttttatatgc gcccaatagc
      541 ggaactgcac gtaaggctct tgagaatcag cctggagcag atgtctggat aacctgggct
      601 gactgggcag ccagtaatcc agaaattggt gatgtcgtgg aaatagcgcc agactacgtg
      661 atatggcgtg atatgaacat tacagtacgt caggatgcaa atgatgaaac ccgtcgattt
      721 gcagaatggc tacaaaccga tgaagcggcg cctgcattca aaaaatatgg ctggaccagg
      781 aaaggcactt gacatcctcg tccttcagga cgtggattct ttttccggat gccgcgccag
      841 cggcatgtag gggcagctca caaaacggaa aaaattgtac gctaagcctc gccaggtgaa
      901 ctgaattcat tccgatatgg gaattcccat atcgggcgaa aacggtttgc tgtaacggca
      961 gagttaagta ggattgctgc gggtgcttga ggctatctgc ctcgggcatg aacaccaacg
     1021 gcagatagat aaaagcccca cccgactata aatcgaagtg aggcccctat atgctcgtca
     1081 catatagatt gcctcttacg gaccgaaagg tcaaggagaa gcaggctatg aagcagcaaa
     1141 aggcgatgtt aatcgccctg atcgtcatct gtttaaccgt catagtgacg gcactggtaa
     1201 cgaggaaaga cctctgcgag gtacgaatcc gaaccggcca gacggaggtc gctgtcttca
     1261 cagcttacga acctgaggag taagagacca ggcgggggag aaatccctcg ccacctctga
     1321 tgtgtcaggc atcctcaacg cacccgcact taacccgctt cggcgggttt ttgtttttat
     1381 tttcaacgcg tttgaagttc cggacggcgc cggaatagaa tcaaaaatac ttaagtagcg
     1441 cgcagggaga agagggatgg accccgaaca ggggagtgct atttatctgg aaggattctg
     1501 ttgatgagaa tcgaagaatt acgtgaaatt tttagtgaag atggcctcta tactgtgcgc
     1561 gttgagaagg gcgctattgt cagccactgc cgtattaaat gtttacagtc tcaacaaagg
     1621 aagagtggag ctgcgttaat tcattttgtg gatgggcttg tgacggatgg ttttattttg
     1681 cgtgcaaatg aatttgtcac atcgttgccg tctctgaaag aagctgggat taaggctggt
     1741 ttttctgctt ttgaagatta gtgaattcat ctacaattca gcgcagggct gaacccctgt
     1801 tgagtaacac tgtgccaccg gagaaagccg atggcgcaaa attccagact acacaattct
     1861 gataattcag ccgtctttgc cagcaggcac gggcggcgtt ctcatgcatt caaatctgac
     1921 tggttccggc acgccccatg cactgaagaa caggccgaat ggctgattca gaactaccgc
     1981 agacgtgggt atgagtttag gaaagccctc agcctcgatt atcgtcactg gataatctac
     2041 gtcaggctgc cgtactccga gcgcccaccg cgtccgtccc gcacattcca gcaacgcatc
     2101 tggaggtaac gtgcgggtat tacttcgacc tgttccggta ccggaacttg ggttggtggt
     2161 ccttaaacca ggccgtgaat ccatgcaggt attccataac cctcgagtgc ttgtggagcc
     2221 ggaaccgaaa agcatgcgcg gtctgccgtc cggcatcgtc cctgccgttc gccagccgct
     2281 ggcggaggat aaatcattac tgccgttttt cagcaatgag cgtgtgattc gtgctgttgg
     2341 tggcgctggt gcactgtctg actggctgtt gcgtcatgtc aaatcctgcc agtggcctca
     2401 tggtgactat catcacagtg aaaccgttat tcaccgttat ggtaccggcg caatggtgtt
     2461 gtgctggcac tgcgacaacc agctgcgtga ccagacatcc gaatcactcg agcaacttgc
     2521 tcatcaaaac ctgtcagcat ggatgattga cgtcatcggt cacgcaataa gcggtacgca
     2581 ggagcgtgaa ttatctctgg ctgaattatc ctggtgggcg gtccgcaatc aggtggcgga
     2641 cgcgctaccg gaagcggtat tacgtcgttc gctggggttg cgtgcggaaa aaatccgctc
     2701 aatgtaccgt gaaagcgaca tcgtaccggg agagcagacc gccaccatca tactgaagca
     2761 gcgcacaaaa aatcttgcgc cgctgcctca cgcccaccag caaaacccgc cacaggaaaa
     2821 gacggtggtc agcattgccg ttgatccgga gtcaccggct cagtatctcc agcgccagaa
     2881 accacaacgg gaagagatgc ctgtatacac gcgctgggta aaaacgcaga aatgcatgac
     2941 gtg
//
LOCUS       DAZFWL020000281         2911 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFWL020000281 DAZFWL020000000
VERSION     DAZFWL020000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.284"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(183..413)
                     /gene="ynfN"
                     /locus_tag="ACHKNP_RS25330"
                     /old_locus_tag="pgaptmp_005066"
     CDS             complement(183..413)
                     /gene="ynfN"
                     /locus_tag="ACHKNP_RS25330"
                     /old_locus_tag="pgaptmp_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HGU4707034.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
     gene            complement(418..483)
                     /gene="ynfR"
                     /locus_tag="ACHKNP_RS25335"
                     /old_locus_tag="pgaptmp_005067"
     CDS             complement(418..483)
                     /gene="ynfR"
                     /locus_tag="ACHKNP_RS25335"
                     /old_locus_tag="pgaptmp_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HGU4707035.1"
                     /translation="MKAPSGAFLLGIYSMDTHILR"
     gene            complement(486..674)
                     /gene="ynfQ"
                     /locus_tag="ACHKNP_RS25340"
                     /old_locus_tag="pgaptmp_005068"
     CDS             complement(486..674)
                     /gene="ynfQ"
                     /locus_tag="ACHKNP_RS25340"
                     /old_locus_tag="pgaptmp_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU4707036.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(685..897)
                     /gene="cspI"
                     /locus_tag="ACHKNP_RS25345"
                     /old_locus_tag="pgaptmp_005069"
     CDS             complement(685..897)
                     /gene="cspI"
                     /locus_tag="ACHKNP_RS25345"
                     /old_locus_tag="pgaptmp_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HGU4707037.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
     gene            complement(1257..1754)
                     /gene="rzpQ"
                     /locus_tag="ACHKNP_RS25350"
                     /old_locus_tag="pgaptmp_005070"
     CDS             complement(1257..1754)
                     /gene="rzpQ"
                     /locus_tag="ACHKNP_RS25350"
                     /old_locus_tag="pgaptmp_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HGU4707038.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
     gene            complement(1751..2284)
                     /gene="rrrQ"
                     /locus_tag="ACHKNP_RS25355"
                     /old_locus_tag="pgaptmp_005071"
     CDS             complement(1751..2284)
                     /gene="rrrQ"
                     /locus_tag="ACHKNP_RS25355"
                     /old_locus_tag="pgaptmp_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4707039.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVESNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            complement(2281..2592)
                     /gene="ydfR"
                     /locus_tag="ACHKNP_RS25360"
                     /old_locus_tag="pgaptmp_005072"
     CDS             complement(2281..2592)
                     /gene="ydfR"
                     /locus_tag="ACHKNP_RS25360"
                     /old_locus_tag="pgaptmp_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HGU4707040.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            complement(2597..2812)
                     /gene="essD"
                     /locus_tag="ACHKNP_RS25365"
                     /old_locus_tag="pgaptmp_005073"
     CDS             complement(2597..2812)
                     /gene="essD"
                     /locus_tag="ACHKNP_RS25365"
                     /old_locus_tag="pgaptmp_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HGU4707041.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
BASE COUNT          834 a          676 c          546 g          855 t
ORIGIN      
        1 tcaatattca tcatttcctc ttttagtctg ttatgacttt ccagttacac agtaagtcga
       61 ttatatggtg caaacgtgta aaagataaga tgaaacatcg caataatcaa catacgatag
      121 tctaaatttt acacaaacag acaaagagaa ttttcctgaa ttatcaatac aatagcatca
      181 aatcaactca agagccttat tgctgcttcc agaatttctt ctgaagtaac atgtcgatcc
      241 gcggctacat aaatgacttt atgatctccg gtcagagatg gaaaccctgc ggccattaca
      301 gtaaggtgtg ttttttcgcc atttggatat tcacgcatga tggtgttaac tccagtcatc
      361 gctggcacta ccactgctgg ttcagagtta aaaaaaacta tgattttttt catgatgtta
      421 ccgtagtatg tgagtatcca tcgaatagat accaagcaaa aaagctcccg aaggagcctt
      481 cattttcact tttttaaatc caacgacaga cggctggcat ttaagtattg tgaaatatta
      541 tcaaatgtaa tcatcattga tttacaaaag atacattttg ccccgaaagg attcatgtca
      601 gaaacatcaa aagatgatgt tctatactgg gaaccatgac aacacgggca tctaaagtga
      661 atatggtttg taatattgtc tacctcaaag cgccactaca tgaacagcgg caggaccttt
      721 aggtccgttc tcaataccaa attcaacttc ctgattctca gttaatgttt tgaaatcgtt
      781 gctctgaatt gctgagaaat ggacaaacac atctttgctg ccatctttcg gcgtgatgaa
      841 accaaaacct ttttcagggt taaaccattt cactaaacca gtcattttgt tagacataat
      901 tattaccttt tgaagaaatt agcccttggg cagaatggtc cgaaaaaaaa tatcagagag
      961 aaaaaccaac aaaatctcaa gaggtacaaa taataaaatt ataacaatga ctgcttcaga
     1021 taaatttgta acaaaccaga acaccattaa cgcatgatta accacccata gcaaggatta
     1081 cttttgtaaa gaaaaacaca gcaatgaaag aatagcttta tttattaata aaacgtgtca
     1141 ttctgattaa gaccttttat cttaccctta agatttcagg aattttggct catggaagag
     1201 tcctttttat ttaaatttta cattccgcga tgtaaatgtt ccgatttaat attaccctac
     1261 atttgatgct ttttatctct taaagattca tagatctgtt gacaagtcac tcctgcgatg
     1321 tagcgttcgt cagcaatttc agcataaagc tgagcttctg ctgcaatatc tccgagcatg
     1381 ttggtgagca ttccttcggc ggttttggtt gttttgcctc tgacggcagc ggcaagatct
     1441 gcggtatgct tcgctgcgtc aaggcgtatg gcatattttt ttgcttcggc acgcaactgg
     1501 ttaacactat cagacagata agcagccctg gcagaaattt cagcagattt ctgttgtgca
     1561 tctttaacag cctcatcacg ggctatagtt cgcccctgtt caattattcg agcagcaaat
     1621 tgagcattta cctcttgtga taatgcggca gcatcacgtt ccgcccattt tttttgccat
     1681 cctctgtcgc tccagacatt tccgacgata aatcctgaca acacgagaaa aatcaccatg
     1741 aatatctgat tcactgttct atcccccagc aggttaatgc gctctcctgg tcacgacgaa
     1801 taacctgacc gtaacagtta tttgaacgaa tgcggcaatc gcgtccgcca tccttaatcc
     1861 accagcgaat cgcttcgcat gcacctttac gatcaccagc attcagccgc ttataaaacg
     1921 tcgacgggaa acacttaccg gggccaatgt tatagggaca aaatgacgcg atacccgctt
     1981 tttgtggttc ggtcagtggt actttaatat tgctctccac ccatgccagc gccttatcac
     2041 gctcaatggc gttgacctgg tcgcattttt ccttcgacag tttcatattg ggaaaaacgg
     2101 tttttccatc caccactgtg gcaccccgac agatggtcca tatgccagaa ccatcgcggt
     2161 atgccattgt gtggttacct tctttttcgt ccagaaactg gtcaagtatc tgaggagcag
     2221 atgcgccagc accaatcagc gccagaacgg cagccgacag gccgtatctg atttttgtgt
     2281 tcatatatat ttatgatgag gacgctcgtg cttattggca ggattttcaa tcttaaagga
     2341 gtactgatgc tgcagataag actcaacttt ttctgacaat ttttctgcta cttccaggaa
     2401 gacttgccgg acgctccttc tggctgctgc ctcataaaac tccagcgcag ctccttcaac
     2461 acggtccatg gcgacatcca ggtcaaaaat ttcaccgtca aagcgttcct tgtcctgtaa
     2521 ggctacagtt accgtaactt tattctcaaa attacggact cctttcacaa ccagttcata
     2581 gtcttgagtc attggattac tctcctctcg cagccttacg cctgtcttct ttaatcttga
     2641 aataaagatt tgtcagatac gtcagcaggc caaaaaccag gctacccagc acaccgattg
     2701 cagcccactg tgacggagtt actttatcga gtaactgtaa tgcccagaaa ccagcattac
     2761 ccgccgatgt gccataggca acacctgttg ttaacttatc cattgatttc atatcctcac
     2821 cccgatgtac acggatggtg caatatgttt gaaaaagatc ggagtctacg gggtagtttt
     2881 gacagcacac gttgttctca acggcgctaa a
//
LOCUS       DAZFWL020000282         2898 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFWL020000282 DAZFWL020000000
VERSION     DAZFWL020000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.285"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1792
                     /locus_tag="ACHKNP_RS25370"
                     /old_locus_tag="pgaptmp_005074"
     CDS             <1..1792
                     /locus_tag="ACHKNP_RS25370"
                     /old_locus_tag="pgaptmp_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707042.1"
                     /translation="GELREFELADGNWLLNNAWYERNMAGFNEQLKENLSFTPDELKT
                     LFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICASCGLTEVHHA
                     PENHKPDPEWYCTSLCRETETLCQEIYERPYNSFISDATANGLILMKLPETWSTNEKM
                     FASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEIQTKYCSTAAR
                     SVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVPGVTDPAEASR
                     LIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASVFWLSTGDRD
                     AALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPTVRLALQKGTG
                     AGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGTQFIRNLAVASSGVAGGA
                     VGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQE
                     QVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSII
                     RQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            1868..2323
                     /locus_tag="ACHKNP_RS25375"
                     /old_locus_tag="pgaptmp_005075"
     CDS             1868..2323
                     /locus_tag="ACHKNP_RS25375"
                     /old_locus_tag="pgaptmp_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HGU4707043.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            2402..2635
                     /locus_tag="ACHKNP_RS25380"
                     /old_locus_tag="pgaptmp_005076"
     CDS             2402..2635
                     /locus_tag="ACHKNP_RS25380"
                     /old_locus_tag="pgaptmp_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGU4707044.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            2735..>2898
                     /locus_tag="ACHKNP_RS25385"
                     /old_locus_tag="pgaptmp_005077"
     CDS             2735..>2898
                     /locus_tag="ACHKNP_RS25385"
                     /old_locus_tag="pgaptmp_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HGU4707045.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLE"
BASE COUNT          742 a          725 c          760 g          671 t
ORIGIN      
        1 gggagagctg agggaatttg aactggcaga cggaaactgg ttactgaaca atgcctggta
       61 tgaaagaaac atggcagggt ttaacgaaca gttgaaagag aacctgtcat tcacacctga
      121 tgaactgaaa acgctcttcc ggaaccgcct gaatttatca ccggaagcga atgacgattt
      181 tctcgatcgt tgcctggacg gcggtgactg gtatcccttt tcagaaggtc gccgttttgt
      241 atcattccat cacgtggatg agcttcgtat ctgtgcctcc tgcgggctga cagaagtaca
      301 tcatgccccg gaaaatcata agccggatcc ggaatggtac tgcacctctc tttgtcgcga
      361 aacagaaaca ctgtgtcagg aaatttatga acgcccttac aacagcttta tttccgatgc
      421 aacggcgaat ggtctgattc tcatgaaact gccggaaacc tggagtacaa atgagaaaat
      481 gtttgcttcc ggagggcagg gacatgggtt tgccgctgaa cggggaaacc atattgtcga
      541 cagagtccgt ctgaaaaacg cacggatcct cggtgataat aatgccagaa atggagcaga
      601 cagactggtc agcggaacag aaatccagac gaaatattgt tcaactgcag cccgtagcgt
      661 cggtgcggca ttcgacggac aaaacggaca gtatcgttac atgggaaata atggccccat
      721 gcaactggaa gtcccccgtg atcagtatgc cggcgctgtt gaaaccatga ggaataagat
      781 ccgcgaaggt aaagtacccg gtgtaaccga tcccgcagaa gcatcccggc tgattcgtcg
      841 gggacatctg acttatactc aggcccgtaa tatcacccgg ttcgggacca tcgaatcggt
      901 cacttatgat attgccgagg gttcggttgt cagtctggcg gccggaggga tcagttttgc
      961 cctgacggca tcggtcttct ggctcagcac cggcgatcgc gatgctgccc tgcagacagc
     1021 tgctgtccag gcaggaaaaa ccttcacccg cacactggct gtctacgtca caacccagca
     1081 actccaccgg ctcagtgttg ttcagggtat gctgaagcac attgattttt cgactgccag
     1141 cccgactgtt cgactggctc ttcagaaggg gaccggtgca ggaaatatca gtgccctgaa
     1201 caaagtgatg aagggtacgc tggtgacatc tctggcactg gtagctgtca caaccggccc
     1261 tgacatgatc aaaatgttgc ggggacggat ctccggtacg cagttcatca ggaatcttgc
     1321 cgtggcatct tccggtgtgg caggtggtgc tgtcgggtca gtggcgggcg gaatactgtt
     1381 cagtccactg ggaccatttg gtgcactgac agggcgtgtg gttggcggtg ttctgggggg
     1441 aatgattgcc tccgctgtat caggaaaaat tgccggagcg ctggttgaag aagatcgcgt
     1501 caaaattctg gcaatgattc aggagcaggt gacatggctt gccggcagtt tcctgctgac
     1561 cggacatgag attgaaaatc tgaacgagaa tctggcccgt gttatcgatc agaatgctct
     1621 ggagatcatt ttcgccgccg gtatacaaca acgggccgcg accaatatgt taatcaaacc
     1681 actggtggtc agtatcatca ggcaacgccc cgtcatggaa tatgatgcat cccatctcgg
     1741 caatatggtt aaccgactgg aagaagcatt acccccggag ttaccggcat aacggctatt
     1801 ctgcgccttt tacgctttat cacctgattt gtcagtgtca tcgtcatgac tcagagaggt
     1861 aaataccatg attcacctgt tcaaaacctg catgattacc gccttcattc tggggttaac
     1921 gtggtctgcc ccactccggg cacaggatca acgttacatc agtatacgca atacagatac
     1981 gatatggctc ccgggaaata tttgtgctta ccagttccgg ctggataatg gcggaaacga
     2041 tgaaggattt ggccccctca ccatcactct gcaactcaaa gacaaatatg gtcagacgct
     2101 ggtgaccaga aaaatggaaa cggaagcttt tggtgacagt aatgccacgc gaaccacaga
     2161 cgcatttctg gaaacggagt gcgtggaaaa tgtcgccaca accgaaatca tcaaagcaac
     2221 tgaagaaagt aacggccatc gtgtcagtct gccgttatcg gttttcaatc cccaggacta
     2281 ccatccactg ctgattaccg tttccggaaa aaacgttaac tgatccctga agtcagtttc
     2341 atgcttcagc ctctgtcaca acgcgtggca gaggcttttt atttatcagg agaaaacaga
     2401 tatgccgggt tacacagagt atgtcctggc tgaaggatct ttctcatacg ggcaggcggt
     2461 tgctgtcata accgcttacc gcaatgtgtt tatccaggat gacccgggaa tgcatttccg
     2521 tcgggttatc cgcaacgcgg aaggtcagcg acgctggcgc tgccggaatt ctgaacccga
     2581 tgccggaaag gtgcttaata cgcggctcgc cagtgacggt cttctcagac aataaacgtc
     2641 attcatttca cccatcaggc cgcgtcttct ccgggagacg tggctttttc atttattaca
     2701 ccagtaaatc ttaaccaccg ataaggagca aattatgcga ctagccagtc gttttggtta
     2761 tgcgaaccag atacgccgtg accgtccgct gacacacgaa gaactgatgc actatgtgcc
     2821 ggggattttc ggggaagata aacacacgtc ccgaagccag aactatacgt acatccccac
     2881 catcaccgta ctggaaag
//
LOCUS       DAZFWL020000283         2879 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFWL020000283 DAZFWL020000000
VERSION     DAZFWL020000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.286"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(106..182)
                     /locus_tag="ACHKNP_RS25390"
                     /old_locus_tag="pgaptmp_005078"
     tRNA            complement(106..182)
                     /locus_tag="ACHKNP_RS25390"
                     /old_locus_tag="pgaptmp_005078"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(146..148),aa:Asp,seq:gtc)
     gene            complement(315..1046)
                     /gene="dnaQ"
                     /locus_tag="ACHKNP_RS25395"
                     /old_locus_tag="pgaptmp_005079"
     CDS             complement(315..1046)
                     /gene="dnaQ"
                     /locus_tag="ACHKNP_RS25395"
                     /old_locus_tag="pgaptmp_005079"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HGU4707046.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1111..1578
                     /gene="rnhA"
                     /locus_tag="ACHKNP_RS25400"
                     /old_locus_tag="pgaptmp_005080"
     CDS             1111..1578
                     /gene="rnhA"
                     /locus_tag="ACHKNP_RS25400"
                     /old_locus_tag="pgaptmp_005080"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HGU4707047.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1575..2297)
                     /locus_tag="ACHKNP_RS25405"
                     /old_locus_tag="pgaptmp_005081"
     CDS             complement(1575..2297)
                     /locus_tag="ACHKNP_RS25405"
                     /old_locus_tag="pgaptmp_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4707048.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2331..>2879
                     /gene="gloB"
                     /locus_tag="ACHKNP_RS25410"
                     /old_locus_tag="pgaptmp_005082"
     CDS             2331..>2879
                     /gene="gloB"
                     /locus_tag="ACHKNP_RS25410"
                     /old_locus_tag="pgaptmp_005082"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HGU4707049.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDL"
BASE COUNT          771 a          704 c          679 g          725 t
ORIGIN      
        1 agcataacaa aaccatagac cgaagaaata gcgggattta aatgcaaaaa aaaccgcaaa
       61 attaaaaatc ttgcggttct ctgaactcat tttcatgagt gaatttggcg gaacggacgg
      121 gactcgaacc cgcgcccccc tgcgtgacag gcaggtattc taaccgactg aactaccgct
      181 ccgcgttgtg ttccgttggg aacgaggcga atattacgga ttgcctcacc cttcgtcaac
      241 ggtttttctc atcttttgaa tcgtttgctg caaaaatcgc ccaagccgct atttttagcg
      301 ccttttacag gtatttatgc ccgccagagg cagcttccgc ccttcttctc caccagatca
      361 agacgggctt catgagctgc aagctcttca tctgtcgcaa aaacaacgcg taacttactt
      421 gcctgacgta caatgcgctg aattgttgct tcaccttgtt gctgctgtgt ctctccttcc
      481 atcgcaaaag ccatcgacgt ttgaccaccg gtcatcgcca gataaacttc cgcaaggatc
      541 tgtgcatcga gtaatgcccc gtgtagcgtt cgtttactgt tatctatttc gtagcgggca
      601 cataacgcat cgaggctgtt gcgcttacca ggaaacattt tcctcgccac cgcaaggcta
      661 tcggtgacct tacagaaggt attggtcttc ggaatatcgc gcttaagcaa cgaaaactcg
      721 tagtccataa agccgatatc gaacgctgcg ttatggatca ccaactccgc gccgcggata
      781 tagtccatga actcatcggc tacttcggca aacgtgggct tatcgagcaa aaattcatcg
      841 gcaataccat gtacgccaaa ggcttccgga tccaccagcc gatcgggttt gagataaaca
      901 tggaagttat tgcccgtcag gcgacggttc accacttcaa cggcaccaat ctcaatgatc
      961 ttgtggcctt catagtgcgc accaatctgg ttcataccgg tggtttcggt atcgagaacg
     1021 atctggcgtg taattgcagt gctcatagcg gtcatttatg tcagacttgt cgttttacag
     1081 ttcgattcaa ttacaggaag tctaccagag atgcttaaac aggtagaaat tttcaccgat
     1141 ggttcgtgtc tgggtaatcc tggacctggg ggttacggcg ctattttacg ctatcgcgga
     1201 cgcgagaaaa cctttagcgc aggctacacc cgcaccacca acaaccgtat ggagttgatg
     1261 gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg aagtcatttt gagtaccgac
     1321 agccagtatg tccgccaagg tatcacccag tggatccata actggaaaaa acgtggctgg
     1381 aaaacagcag acaaaaaacc agtaaaaaat gtcgatctct ggcaacgtct tgatgctgca
     1441 ttggggcagc atcaaatcaa atgggaatgg gttaaaggcc atgccggaca cccggaaaac
     1501 gaacgctgtg atgaactggc ccgtgctgcg gcaatgaatc ccacactgga agatacaggc
     1561 tatcaagtcg aagtttaagc ctgtggttta cgatattgcc gggtggctcc aaccgcctgg
     1621 cgaattcgtg gcttgttttt actctgtttc atcggattta gcgttaaagg aatagtccgt
     1681 ttccgggcaa caataagttg taagcaacca agcgcaggaa tatgtgcatt caatagtttt
     1741 ccaccgtgtt tgttccacgg gagaacgtgg aaacggctgg cgtgcagcac ttcaaaattc
     1801 aacaaagaga gccagtccag ttgccgcatc agagtaaaca tccggctgtt atagggcgag
     1861 gtttttcgca ataccggcac aagtttgcgt aatcccatta aactaatggg attgaagcca
     1921 ctaatcacca gccagccatc atcaatcaat acccgatcgg tttcacgcaa taaacgatgc
     1981 ggatcggtgc accacggcaa tgtatgtgcc agtagacaaa catcaacgga tttatcggca
     2041 aaaggaagat gaagtggatc cgcctgcacc tggacgggca ttccttgcgc agaaacattc
     2101 acttgatgag aaaccgcgca cgcttcgcaa ttgatttctg cgcttaaatt gccaatctta
     2161 agcagatgaa aaccatacat tttagtgaac cacgggttga gctggcgctc cagcgccttg
     2221 cgataaagct ctccccaggg caaatcgccc cagcgatcag gagccacgac agtttgaggg
     2281 actcttgccg gtttcatcac aaccttccgt ttcacactaa gaggtaatct atgaatctta
     2341 acagtattcc cgcctttgat gacaattaca tctgggtttt gaatgatgaa gcaggtcgct
     2401 gcctgattgt cgatcccgga gacgcagagc cagtattaaa cgccattgcc gccaataact
     2461 ggcaaccgga ggccatattt ctcacccacc atcatcacga tcacgttggc ggcgtaaaag
     2521 aactggtgga aaagtttccg caaattgtgg tgtatggtcc acaagagaca caagataagg
     2581 gaacaacaca ggtagtcaaa gatggcgaaa ctgccttcgt tttggggcat gaatttagtg
     2641 taattgccac accgggtcac actttaggac atatctgtta cttcagtaaa ccttatctat
     2701 tttgcggcga cacgctgttt tctggtgggt gtggtagatt gtttgaaggg acagcatcac
     2761 aaatgtatca atcacttaat aagttaagtg cgttacctga cgatacattg gtatgttgtg
     2821 ctcatgaata tacgttatca aatatgaagt ttgctttgag tattcttccg cacgatttg
//
LOCUS       DAZFWL020000284         2831 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFWL020000284 DAZFWL020000000
VERSION     DAZFWL020000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.287"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..95
                     /gene="rrf"
                     /locus_tag="ACHKNP_RS25415"
                     /old_locus_tag="pgaptmp_005083"
     rRNA            <1..95
                     /gene="rrf"
                     /locus_tag="ACHKNP_RS25415"
                     /old_locus_tag="pgaptmp_005083"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(159..917)
                     /gene="yhdZ"
                     /locus_tag="ACHKNP_RS25420"
                     /old_locus_tag="pgaptmp_005084"
     CDS             complement(159..917)
                     /gene="yhdZ"
                     /locus_tag="ACHKNP_RS25420"
                     /old_locus_tag="pgaptmp_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGU4707050.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(925..2028)
                     /gene="yhdY"
                     /locus_tag="ACHKNP_RS25425"
                     /old_locus_tag="pgaptmp_005085"
     CDS             complement(925..2028)
                     /gene="yhdY"
                     /locus_tag="ACHKNP_RS25425"
                     /old_locus_tag="pgaptmp_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU4707051.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2038..>2831)
                     /locus_tag="ACHKNP_RS25430"
                     /old_locus_tag="pgaptmp_005086"
     CDS             complement(2038..>2831)
                     /locus_tag="ACHKNP_RS25430"
                     /old_locus_tag="pgaptmp_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU4707052.1"
                     /translation="FRNIPPLLQIFFWYFAVLRNLPGPRQAVSAFDLAFLSNRGLYIP
                     SPQLGDGFIAFILAVVMAIILSVGLFRFNKTYQIKTGQLRRTWPIAAVLIIGLPLLAQ
                     WLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVPYG
                     QHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAAIGYPDMVSLF
                     AGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          777 a          761 c          678 g          615 t
ORIGIN      
        1 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
       61 gggtctcccc atgcgagagt agggaactgc cagacatcaa atcaagcaaa aaggccatcc
      121 gaaaggatgg cctttttgct tttcgaacta acattcaatt aatggattac ctgcgataaa
      181 aatgccctcg tacgctctga tttaggatgc gtaaaaaatt catcaggtgc agcttgctcc
      241 actatttccc cacgatccat aaaaatcacc cggtcagcga cggttcgcgc aaaccccatc
      301 tcatgcgtta cacacaacat tgtcataccc gattgcgcca gcccaatcat cgtatccagc
      361 acctctttca ccatctccgg atcgagcgcc gacgttggct catcaaacaa cataattttc
      421 ggcttcatac acagcgaacg cgcaatggca acgcgttgct gctgaccacc agaaatttgt
      481 ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct caaggtaatg catcgccaga
      541 gcttcagcct ctttctttgg catcttgcgt acccaaatcg gtgccagggt acagttctgt
      601 agaacggtca gatgagggaa gagattgaaa tgctgaaaga ccattcccac ttcctgccgg
      661 acgcgctcaa tattgcggat atcttcatta agttcgatgc catctaccac gatccgtccc
      721 tgttgatgtt cttccagatg attaatacaa cgaatggttg tcgatttacc ggaacctgaa
      781 gggccacaca gaacgatccg ttctcccggt tgcacggtta aatttatatt tttcaaaaca
      841 tggaattgtc cataccattt attgacgttt tccagcgtaa tcatcgcgtt agcaggttgc
      901 agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc ccggtgttaa aacgtttttc
      961 cagatgctgg ctatagcgcg acatgctgaa acagaagatc cagtagatca gcgcggcgaa
     1021 aacataccct tccgtcgaca tacccaacca ggtgggatca acggttgcct gctgaacgct
     1081 actgaaaaga tcgaacaacc cgataatgat caccaggctg gtatctttga agagtgcgat
     1141 gatggtattt accagcccag gaattaccag cttcaacgcc tgtggcagaa taaccagccc
     1201 ctgagttttc cagtaaccca acgccagcga ctctgccgct tcatactgcc ctttaggcag
     1261 tgcctgtaat ccgcctcgca cgacttccgc aacatatgct gactgaaaca ggatcacgcc
     1321 aaccagcgcg cggatcagtt tgtcgatact ggtgccttct gccataaaca acggcagcat
     1381 gaccgaagac ataaacaata cggtaatcag cggtacgccg cgccagaact cgataaaaat
     1441 gaccgataag atacgcacaa tcggcatatg ggagcggcga cctaacgcca gtaagatccc
     1501 ccacggtagc gcccccgcaa tcccaacgga tgcaataatt aaagttagcg tcagcccgcc
     1561 ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg ccatacatca gccaccagac
     1621 aatcagtggg taaatcaccg cccagaccgc aatatagcga ccgcgatgcg ggagtatttt
     1681 ccagaacatt ggtgcgattg acacaaggcc aatcagtaat gccaggttaa ttcgccagcg
     1741 ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat cgttcgtgga tgaagaccca
     1801 acaggcaccg gcttttgtgc agtcggcacg cgttgagcca acccaattag cctgcaaaaa
     1861 tgcccagttc agcaacggtg ggatcagttc ccacatcaac catatgcagc caatagtcag
     1921 caggctattg ctccagctgg agaacagatt ttttcgcacc cacaccatcg cgcggcttga
     1981 gttatggctc gctgggcgcg agggctgaga cagcaatact tttgtcatca tgactcctta
     2041 gcgttcaacg atcgcgatgc ggcggttata gatattcatc agcagcgaga tagtcaggct
     2101 gataatcaga tagaccgaca tggtcatggc tatcgtctcg atggcttgcc ccgtctggtt
     2161 cagcacggtg ccagcaaaca gcgaaaccat atcgggatag ccaatagcgg cggcaagaga
     2221 ggagtttttg acgatgttga gatactggct ggttaacggc ggaataatca ctcgcagtgc
     2281 ctggggaata atgacctggc gtagcgtaac cgggttgggt aatcccagtg accgagccgc
     2341 ttcatgttga ccataaggca ctgcctggat cccagcgcgg ataatctcgg cgataaatgc
     2401 agatgtataa accgaaagtg ccagcgttaa ggctgccagt tcaggaatta aaaccatccc
     2461 gccgcggaag ttaaagcctc gtagggctgg gacatcccag tgtagtgctg cgccaaaaag
     2521 ccattgcgcc agtaaaggca aaccaatgat caacactgcg gcgatcggcc aggtgcggcg
     2581 cagttgtccg gtctttatct ggtatgtttt attaaagcgg aatagcccaa cagaaaggat
     2641 tatagccata acaacagcca ggataaacgc aataaatccg tctcccagct gcggtgacgg
     2701 aatataaagc ccacgattgc tcaaaaaggc cagatcaaac gcgctcacag cctggcgtgg
     2761 tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag atttgcagca gcgggggaat
     2821 attacggaag a
//
LOCUS       DAZFWL020000285         2799 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFWL020000285 DAZFWL020000000
VERSION     DAZFWL020000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.288"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..567)
                     /locus_tag="ACHKNP_RS25435"
                     /old_locus_tag="pgaptmp_005087"
     CDS             complement(<1..567)
                     /locus_tag="ACHKNP_RS25435"
                     /old_locus_tag="pgaptmp_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707053.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLL"
     gene            complement(654..860)
                     /locus_tag="ACHKNP_RS25440"
                     /old_locus_tag="pgaptmp_005088"
     CDS             complement(654..860)
                     /locus_tag="ACHKNP_RS25440"
                     /old_locus_tag="pgaptmp_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4707054.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1612..1761
                     /locus_tag="ACHKNP_RS25445"
                     /old_locus_tag="pgaptmp_005089"
     CDS             1612..1761
                     /locus_tag="ACHKNP_RS25445"
                     /old_locus_tag="pgaptmp_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HGU4707055.1"
                     /translation="MEKVISHTRYKLTPAEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVI"
     gene            2573..>2799
                     /locus_tag="ACHKNP_RS25450"
                     /old_locus_tag="pgaptmp_005090"
     CDS             2573..>2799
                     /locus_tag="ACHKNP_RS25450"
                     /old_locus_tag="pgaptmp_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4707056.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPT"
BASE COUNT          733 a          657 c          744 g          665 t
ORIGIN      
        1 caggagcacg tactgatcca tcagatccgt cagtgctgcg ctctgcttct gacggttgat
       61 gatccattcc tccagctctt tttctgattt ccgtttcaga ttctccgtcc agtcgtcaag
      121 gcagtttccg gtcattaaat caagccagca aagccagacc agtttgaggc gcaactccgt
      181 gccaccaaac tgacggaaga ggctgatcag tttatttgcc agcgaacgct gcctcagacg
      241 ttcacattca gacaggagaa tgccttccag catcggatga tgaaccagca gatgttcact
      301 ggtgcataaa ttcactatca gatcatcatc caggggcggc gtggtccgtt tgtccggacc
      361 ataaatcagc cggtgcagac tctggggtac gatggcaatc tccggttttt caccattgaa
      421 tgacacccac ccgggcgtca gattatggaa gtagtagata ttcggatgac acacgcccca
      481 ggatacggcg tgccgggtga agccggtcag ccatttctgg tagctttcgt ggagcagctc
      541 gtaacgggtc tgatactgtg gtttcatttt catattcctc acgggggata acaggcagca
      601 gaacgccgcc ggcaggccgg agactgcgtc tgctgcagat gaacggttaa gaattacgga
      661 cgggactgtg caatacgcgc ctgcagccag gcttccactt cgcttttcag ccatcgggag
      721 ctgcggccca ttttgatggg cgcaggaaag tcaccatcct tgatgagttt gtaaaaccac
      781 ttatcggtca ggccggtcag ttgagtgata aatgccatgt cgaccatctg gtcatccatc
      841 agtgaaactg gggtgatcat gagaatgttc ctccggatgt tgttaaacca gccggtgtgg
      901 tcatgaaaac aacgcaccac cttcaccggc ataacaaccg gggagaaacg taaaaaataa
      961 tcgggaatgt gaccgccagg agataaacgg ggaggttacg gtggctgagc cgtaccggta
     1021 tgccgggtgg ttgttacgaa acgatgcttc cgcacgcgcg cgcgataaag aagggatgat
     1081 tttcgtgagg aagaggggaa aactggcgtt ctgataattg cagagttatt tacggggcgg
     1141 ttacgatggg gcagaccgtg tccggtgggc aggcgacagg tatcaccgat gggataagac
     1201 tgtctgattg gtattataaa tatataagag agatcaaaca catgagatca aatacatgag
     1261 ttcatatatg tgaaataaac acggtgagtg tgatcagtgg gcatgatgag aacgcagtag
     1321 caacagactg cgctatccct gatagctggc gtataacctg cgtatataaa acgtaccagc
     1381 aacgcacgtg aacagagtgc attaagcatc cgtcgttgac accgacctgg tgagtgacag
     1441 tctgagagac cggttaagtg gtggtacggg agtaatgagt ttggggaggg taccagaatg
     1501 tcagtctggt acggtatgct gacagtatca cgttaattgc agcataaagt gatgagaggc
     1561 taatggaagt taaaaccaga gcagttccgt cgttgttcat cccgggagac actggaaaaa
     1621 gtgatttccc acacgcgtta taagcttact ccagcggaga tggaagcctt caactctgcg
     1681 gtcgatcacc gactggcaga actgacaatg aacaagcttt acgatcgtgt tcccgcttca
     1741 gtctggaaat atgtcatctg agcgttcggt aatcacaact gcatcgcgct aggtgttatc
     1801 cgctttgtga ttcttattgg ctcttctcag gttattaaaa gctgaatatg ttcctgttgt
     1861 cgggaaccag gataataccg ggagcgttaa gcggtatagc gacaacaaag aattcgaaat
     1921 caaaagggaa tggtgccgga ggcgtgggcg gtaataggct gattttcagg tattaccggc
     1981 ttacaacgtg tggttgatat tgaggggctg tgagtttcgc gtatttgtca gaaacccaga
     2041 tgagttttcc tgtatgggac gaaataaggc agcggcaata cctggaaaat ttaatatggt
     2101 atgaaggaga acctgtcggt tacgcttaat atacacggta cactgtcagc tcaggggata
     2161 catgaaccgt tatctcacca gctacacagt atacacacac tgacgctgac agtagtcagc
     2221 aggaccacaa taatcagcgc cagcagtatg gtaacgatac ggtctgtatt ccggtagtag
     2281 ccgaaggggt cgacagaacc acaatacggg caacacactg tattattagt aatcgcgttt
     2341 ccacagtccc tgcacttctt ttgcgtatca ccataactcc ctcctcacag ccttacccgt
     2401 aaccggtttt ttacattaaa aaacaacgct gaaaattcaa ccgtcagtcc ggagacgacc
     2461 gttcgggtta tcacagagtg cctgagacag tgtcctgtcg gaggtcactt cagggactct
     2521 gcgcattttt tacgacgtgg ctattccgta tgaaccatac ggagatttaa ccatgaccta
     2581 caaatacaac cccttctggc agcaacgtat tcgtgagacg gtgcggcacg cactggatgt
     2641 tcatccccgt ctgacggcat tgcgggttga tctgcgtctc ccggatgtac cggcagcaac
     2701 ggacgcagct gtgatatccc gcttcatcaa tgccctgaaa gcccgaatcg acgcttacca
     2761 gaaacgtaag catcgggaag gtaaacgcgt gcatcccac
//
LOCUS       DAZFWL020000286         2791 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFWL020000286 DAZFWL020000000
VERSION     DAZFWL020000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.289"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..226)
                     /locus_tag="ACHKNP_RS25455"
                     /old_locus_tag="pgaptmp_005091"
     CDS             complement(<1..226)
                     /locus_tag="ACHKNP_RS25455"
                     /old_locus_tag="pgaptmp_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707057.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQ"
     gene            complement(235..327)
                     /locus_tag="ACHKNP_RS25460"
                     /old_locus_tag="pgaptmp_005092"
                     /pseudo
     CDS             complement(235..327)
                     /locus_tag="ACHKNP_RS25460"
                     /old_locus_tag="pgaptmp_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            complement(360..698)
                     /locus_tag="ACHKNP_RS25465"
                     /old_locus_tag="pgaptmp_005093"
     CDS             complement(360..698)
                     /locus_tag="ACHKNP_RS25465"
                     /old_locus_tag="pgaptmp_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4707058.1"
                     /translation="MLMVKKTIYVNPDRGQNRKVSDRGLTSRDRRRIARWEKRIAYAL
                     KNGVTPGFNAIDDGPEYKINEDPMDKVDKALATPFPRDVEKIEDEKYEDVMHRVVNHA
                     HQRNPNKKWS"
     gene            complement(701..1006)
                     /locus_tag="ACHKNP_RS25470"
                     /old_locus_tag="pgaptmp_005094"
     CDS             complement(701..1006)
                     /locus_tag="ACHKNP_RS25470"
                     /old_locus_tag="pgaptmp_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HGU4707059.1"
                     /translation="MVTIVWKESKGTAKSRYKARRAELIAERRSNEALARKIALKLSG
                     CVRADKAASLGSLCCKKKEEVVRKNRSIYYKDSNPLGNKIHAVQKIKLYSKLPYGAY"
     gene            complement(1321..1971)
                     /locus_tag="ACHKNP_RS25475"
                     /old_locus_tag="pgaptmp_005095"
     CDS             complement(1321..1971)
                     /locus_tag="ACHKNP_RS25475"
                     /old_locus_tag="pgaptmp_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU4707060.1"
                     /translation="MNTQLMGERIRARRKELKIRQAALGKMVGVSNVAISQWERSETE
                     PNGENLLALAKALQCSPDYLLKGEDSLSNIAYHSRHDPRGSYPLISWVSAGCWMEAVE
                     PYHRRAIDNWYDTTVDCSEDSFWLDVKGDSMTAPAGLSIPEGMIILVDPEVEPRNGKL
                     VVAKLEGENEATFKKLVIDAGRKFLKPLNPQYPMIEINGNCKIIGVVVDAKLANLP"
     gene            2052..2237
                     /locus_tag="ACHKNP_RS25480"
                     /old_locus_tag="pgaptmp_005096"
     CDS             2052..2237
                     /locus_tag="ACHKNP_RS25480"
                     /old_locus_tag="pgaptmp_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU4707061.1"
                     /translation="MYKKDVIDHFGTQRAVAKALGISDAAVSQWKEVIPEKDAYRLEI
                     VTAGALKYQESAYRQAA"
     gene            2347..2643
                     /locus_tag="ACHKNP_RS25485"
                     /old_locus_tag="pgaptmp_005097"
     CDS             2347..2643
                     /locus_tag="ACHKNP_RS25485"
                     /old_locus_tag="pgaptmp_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HGU4707062.1"
                     /translation="MAQASYSKLTQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPVAAGDSKQIDMQF"
     gene            2676..>2791
                     /locus_tag="ACHKNP_RS25490"
                     /old_locus_tag="pgaptmp_005098"
     CDS             2676..>2791
                     /locus_tag="ACHKNP_RS25490"
                     /old_locus_tag="pgaptmp_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU4707063.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLIEA"
BASE COUNT          734 a          626 c          572 g          859 t
ORIGIN      
        1 tttgtttcca ttcctgtaac cactgatttg gtgctggttt aaaattaaca atccaatgcg
       61 caggaaccaa ccatgcataa tgctctgtct gatgaaaagc tatatattga agtgcgaata
      121 tttttatccc atcttcttca actgtcgcct ggaatctcca gaaaacaggc attccatcat
      181 gttcagtttc tgattcagga aaaggtacgc tccatgattt tgtcatatct cacctcaaat
      241 aagtggtttg ctgcctaatt tcattttctg gcgaccaaca caagtcacac ccatttcact
      301 gcgtggcttg ctgtaataaa ttaggttagt tcagccaata aaaaacccac cgaagtgggc
      361 tatgaccatt ttttatttgg atttcgttgg tgagcgtgat taacaactct gtgcattaca
      421 tcctcatatt tttcatcttc aattttttcg acatcgcgag gaaatggtgt tgctaatgct
      481 ttgtcaactt tgtccattgg gtcttcatta atcttatatt caggaccgtc atctatagca
      541 ttaaatccag gtgttacacc gttttttaat gcatatgcta tcctcttttc ccatctcgct
      601 attctcctcc tgtctcgaga tgtaagacct ctatcagata cttttctgtt ttgtccgcgg
      661 tcaggattaa cataaatagt ctttttcacc ataagcatac tcaataagca ccgtacggta
      721 gtttactgta caattttatt ttttggactg catgtatttt gtttcctaat gggtttgaat
      781 ccttgtaata aatacttcta ttttttcgaa caacttcttc tttcttcttg cagcaaaggc
      841 ttcctagtga tgctgctttg tctgctctga cgcaaccaga gagctttagc gcaatttttc
      901 gcgccagtgc ttcattactg cgtcgctcgg caataagttc tgctctgcga gctttgtagc
      961 ggctttttgc cgtacctttg gattctttcc agacaatggt taccatgatg gtctccttta
     1021 agtggctttg gcgcatgacg cgtcgaggtg cttatcttct cgatcgctgt cttgcagctg
     1081 caattcgcgc catccccaaa accactcaag ttctggtctc aacggttagg ttgagagttc
     1141 gtcgatgtta aagagcctgc caatctgttc cgtttggctt ccagcctcct gctgacggct
     1201 taaaattaag acctcttaat tttttggtca agtgcatttt tgaagaaaac ttaattttat
     1261 gggcgcgaat ttagtttgtc tttgattttt aacgggaaat aaaaaagggg cgaaagcccc
     1321 ttaaggaagg tttgctagct tggcatcaac gacaacgcca atgattttac agttcccatt
     1381 gatttcaatc attgggtatt gtggattgag tggtttcagg aattttctac cggcatcaat
     1441 aactaacttt ttgaatgtcg cctcgttttc tccttcaagt ttggcgacta ccagctttcc
     1501 attacgtggt tcgacttctg ggtcgacgag aataatcatc ccttcaggaa tactcagtcc
     1561 tgccggggca gtcattgaat cacctttaac gtcgagccaa aaagagtctt cagaacaatc
     1621 taccgttgtg tcgtaccagt tatctattgc acgcctatga tatggctcta cagcttccat
     1681 ccaacatcct gcgcttaccc aactaattag aggatacgaa cctcttggat catgcctgct
     1741 gtgataggca atgtttgaaa gactatcctc tcctttcaac aggtaatcag gggagcactg
     1801 caaagccttg gctaaggcca ataggttttc gccattgggc tcagtttcag atcgctccca
     1861 ttgggaaata gcaacattag acacgccaac catcttgcca agggcagcct gcctaatctt
     1921 gagttctttt ctgcgagcgc gaatacgctc acccatcagt tgtgtattca tagttaagac
     1981 atcttaaata aacttgactt aagattcctt tggtggataa tttaagtatt ctttaatttc
     2041 ggagcgagtc tatgtacaaa aaagatgtta ttgaccactt cggaacccag cgtgctgttg
     2101 ctaaagcact aggcattagc gatgcagcag tctctcagtg gaaagaagtt atcccagaga
     2161 aagacgccta tcgattggaa atcgttacag ctggcgccct gaagtatcaa gaaagtgctt
     2221 accgccaagc ggcataagta aattgctctt taacagttct ggcctttcac ctctaaccgg
     2281 gtgagcaaac atcagcggca aatccactgg gtgtgccgct atatatcaat ataggaaaat
     2341 taacaaatgg cacaagcaag ttacagcaag ctaacacagc gagaaattga tcgcgcagaa
     2401 acagatttac tcatcaacct gtcaacgctt acccagcgcg gtctggcaaa gatgattggc
     2461 tgtcatgaat cgaagataag cagaacggac tggagattta ttgcttcggt cttgtgtgct
     2521 ttcggaatgg catcagacat cagtccgatt agcagggctt ttaagtatgc gcttgatgaa
     2581 atcacaaaga aaaaatcccc ggtggccgcc ggggactcta agcaaattga tatgcaattc
     2641 tgagggaatt actggatcaa tccacaggag tcattatgac aaatacagca aaaatactca
     2701 acttcggcag aggtaacttt gccgaacagg agcgtaatgt ggcagatctc gatgatggtt
     2761 acgccagact atcaaatatg ctgattgagg c
//
LOCUS       DAZFWL020000287         2738 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFWL020000287 DAZFWL020000000
VERSION     DAZFWL020000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.290"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            102..1076
                     /gene="cysB"
                     /locus_tag="ACHKNP_RS25495"
                     /old_locus_tag="pgaptmp_005099"
     CDS             102..1076
                     /gene="cysB"
                     /locus_tag="ACHKNP_RS25495"
                     /old_locus_tag="pgaptmp_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HGU4707064.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1395..1535
                     /locus_tag="ACHKNP_RS25500"
                     /old_locus_tag="pgaptmp_005100"
     CDS             1395..1535
                     /locus_tag="ACHKNP_RS25500"
                     /old_locus_tag="pgaptmp_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HGU4707065.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            1538..1705
                     /gene="yciX"
                     /locus_tag="ACHKNP_RS25505"
                     /old_locus_tag="pgaptmp_005101"
     CDS             1538..1705
                     /gene="yciX"
                     /locus_tag="ACHKNP_RS25505"
                     /old_locus_tag="pgaptmp_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HGU4707066.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1819..1914
                     /gene="ymiC"
                     /locus_tag="ACHKNP_RS25510"
                     /old_locus_tag="pgaptmp_005102"
     CDS             1819..1914
                     /gene="ymiC"
                     /locus_tag="ACHKNP_RS25510"
                     /old_locus_tag="pgaptmp_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HGU4707067.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2078..>2738
                     /locus_tag="ACHKNP_RS25515"
                     /old_locus_tag="pgaptmp_005103"
     CDS             2078..>2738
                     /locus_tag="ACHKNP_RS25515"
                     /old_locus_tag="pgaptmp_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HGU4707068.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NG"
BASE COUNT          708 a          635 c          645 g          750 t
ORIGIN      
        1 tggttatagt tagcaccttt tttattatta aatcgtatta gtcacccgcc aggtgtgacg
       61 aaaaaacgat gttctgatgg cgtctaagtg gatggtttaa catgaaatta caacaacttc
      121 gctatattgt tgaggtggtc aatcataacc tgaatgtctc atcaacagcg gaaggacttt
      181 acacatcaca acccgggatc agtaaacaag tcagaatgct ggaagacgag ctaggcattc
      241 aaattttttc ccgaagcggc aagcacctga cgcaggtaac gccagcagga caagaaataa
      301 ttcgtatcgc tcgcgaagtc ctgtcgaaag tcgatgccat aaaatcggtt gccggtgagc
      361 acacctggcc ggataaaggc tcgctgtata tcgccaccac gcatacccag gcacgctacg
      421 cgttaccaaa cgttatcaaa ggctttattg agcgttatcc ccgcgtttct ttgcatatgc
      481 accagggctc gccgacacaa attgctgatg ccgtctctaa aggcaatgct gatttcgcta
      541 tcgccacaga agcgctgcat ctgtatgaag atttagtgat gttaccgtgc taccactgga
      601 atcgggctat tgtagtcact ccggatcacc cgctggcagg caaaaaagcc attaccattg
      661 aagaactggc gcaatatccg ttggtgacat ataccttcgg ctttaccgga cgttcagaac
      721 tggatactgc ctttaaccgc gcagggttaa cgccgcgtat cgtcttcacg gcaacggatg
      781 ctgacgtcat taaaacttac gtccggttag ggctgggggt aggggtcatt gccagcatgg
      841 cggtggatcc ggtcgccgat cccgaccttg tgcgtgttga tgctcacgat atcttcagcc
      901 acagtacaac caaaattggt tttcgccgta gtactttctt gcgcagttat atgtatgatt
      961 tcattcagcg ttttgcaccg catttaacgc gtgatgtcgt tgatgcggct gtcgcattgc
     1021 gctctaatga agaaattgag gtcatgttta aagatataaa actgccggaa aaataatttc
     1081 ttgcgttatt ttcggcacct tttatgtagc gaaggtgccg gaatatattc tcttttgtta
     1141 cttatttcta taccgtgcac aatattttta tccctcatat ttatagggta aattacctgt
     1201 aaagtagtgt ctaattaccc gcctcgcaaa ctccagtttt caaatatcta tttcaggtca
     1261 aaagattgaa ttattcactg gagacgattc gtaaattcgc tggatttttg actaaagttt
     1321 ctttaggatt tgtctcatcg tatgattaat tacaccaatc gttcaacgtc ttgctgataa
     1381 gtgatggcga ttatatgagg ttagcaatgc cttctggaaa tcaggaaccc cgccgcgatc
     1441 ctgaattaaa acgtaaagcc tggctggcgg tttttcttgg ttctgcactt ttctgggtgg
     1501 ttgtcgcact gctgatttgg aaagtgtggg gataactatg gtcggtcagg agcaactgga
     1561 gtcgtcacca ttatgccagc atagtgacaa tgaaacagaa acgaaacggg aatgttccgt
     1621 cgttattcca gacgactggc aactaacatc gcagcagcaa gcctttatag aactgtttgc
     1681 tgaagatgat cagccgaaac aataattatc atcattctta ttacccattt ttaatgaatt
     1741 aaagggcttt taatacaccg cagcaataac agcttgagtt atctcaacac aaaataataa
     1801 ccgttaaggg tgtagcctat gatcaacaca aatatgaaat attggtcctg gatgggcgcg
     1861 ttttctctgt cgatgctctt ctgggccgaa ctcctctgga tcattactca ctgatccttg
     1921 accccgctgc ggcggggttg tcatttgctt tgccacaagg tttctcctct tttatcaatt
     1981 tgggttgtta tcaaatcgtt acgcgatgtt tgtgttatct ttaatattca ccctgaagag
     2041 aatcagggct tcgcaaccct gtcattaagg aggagctatg tcgtcaaccc tacgagaagc
     2101 cagtaaggac acgttgcagg ccaaagataa aacttaccac tactacagcc tgccgcttgc
     2161 tgctaaatca ctgggcgata tcacccgtct acccaagtca ctcaaagttt tgctcgaaaa
     2221 cctgctgcgc tggcaggatg gtaactcggt taccgaagag gatatccacg cgctggcagg
     2281 atggctgaaa aatgcccatg ctgaccgtga aattgcctac cgcccggcaa gggtgctgat
     2341 gcaggacttt accggcgtac ctgccgttgt tgatctggcg gcaatgcgcg aagcggttaa
     2401 acgcctcggc ggcgatactg caaaggttaa cccgctctca ccggtcgacc tggtcattga
     2461 ccactcggtg accgtcgatc gttttggtga tgatgaggca tttgaagaaa acgtacgcct
     2521 ggaaatggag cgcaaccacg aacgttatgt gttcctgaaa tggggaaagc aagcgttcag
     2581 tcggtttagc gtcgtgccgc caggcacagg catttgccat caggttaacc tcgaatatct
     2641 cggcaaagca gtgtggagtg aattgcagga cggtgaatgg attgcttatc cggatacact
     2701 cgttggtact gactcgcaca ccactatgat caacggcc
//
LOCUS       DAZFWL020000288         2725 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFWL020000288 DAZFWL020000000
VERSION     DAZFWL020000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.291"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>2725)
                     /locus_tag="ACHKNP_RS25520"
                     /old_locus_tag="pgaptmp_005104"
     CDS             complement(<1..>2725)
                     /locus_tag="ACHKNP_RS25520"
                     /old_locus_tag="pgaptmp_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707069.1"
                     /translation="PRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTAL
                     VGVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWC
                     LWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTELRITCNAYLTTQR
                     KAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSA
                     LKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGL
                     WLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTL
                     DREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPT
                     LRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQH
                     LTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFR
                     QLALGRYTLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYD
                     PTVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYICSVNTVGK
                     SAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVS
                     NEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIE
                     NTSDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGN
                     PPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCRVLGKLSANQIEGDLVKTV
                     GKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEREHTDI"
BASE COUNT          536 a          853 c          706 g          630 t
ORIGIN      
        1 aaatatcagt atgctctctc tcatgtttag cgccgctgaa tgccaccgcc ggaataacaa
       61 tctgccggtc aaacggctga tcgtcataaa ccctgacggt aatggtccct gatggccacc
      121 gttccggtgc acgggagtcc cgggggaaag ctttgcccac tgttttaacg agatcgcctt
      181 caatctggtt cgcggacagt tttcccagaa cccgacagtt ctcgttaatc gtgacattgt
      241 tgagcgtccc ggcgttcgca ttcacactgc cactgatatc cgcattttta gcggtcagct
      301 ttccgtccgg tgtcagggaa aatgccggtg gatttccacc gctggtaatg gtgggggccg
      361 tcaggcgttt caggaacacg tcgttcatga atatctgatc gccctgacca acaaacatcg
      421 gttttgtgtt gccattcgca ggattaacca tcgcaatcct gtccgccgcc agcagcacct
      481 gactctgcat gccgtcagag gtgttctcaa taccggcacc aatacccgcg atataaaggc
      541 gtccgtcctg catctgctgc agcttcacag cccacatgct gttcaggtta ttatttgtat
      601 caacctgaac cttctgtatc tgctgaattg ccgcactctg gtcttccagt ttcttattga
      661 cggtctgcgt gatttcattg ctgacatccg taatggacgt cctgatttca gccaggtcag
      721 gcgcaagctg accgttatca atctgcgtcc acaactcctg agccagatgg gttttcccta
      781 tctcgccttt gaaaaaatcc agatagccgg atgcatcatc actcggctgg ccgacggcct
      841 ccacaaatgc cgatttgcca acggtgttca cactgcagat gtaaaaataa taatcatggc
      901 ccggcttaat attgatactg gcagctatcc agtacagcgc cgtgccaaga tagcgggctg
      961 tggtttcaac ctgcctgata tccgcaatcc gcttttccga gaaccagaat tcaaactgta
     1021 ccgtcggatc ataaaccgca agatgcggcg tggcggttat ctgaaaatag cccggcgtca
     1081 gctcaatccg cgacggcgct gccggtgcgg caatccggaa cgataccgac gccggatcgc
     1141 cctgctgtcc ccacgcattt accgcccgga ctgtcagcgt gtaacgcccc agcgccagtt
     1201 gcctgaagcg gtatgtggtt tccgtcgtcc gggccgtgct gaccagccgc tcactgccgt
     1261 catccgctgc cacggtcagg cgaagaagga agctaacgcc cttcaccacc ttcggcgtgt
     1321 cccagcgcgc cagcacctga tattccccgc tgtctgcggt gacttcggca gtcaggtgct
     1381 gtaccgctgg cggcgtgaca ccattcaccg tgccgctctg gtcgccgtca aagtgcgccc
     1441 cgttatccac gatggcttct ttttccggta catgctgcac ggcagtgatg gcatacgtgc
     1501 cgtcatcgtt ctcacggata ctcacgcagc ggaacaggcg ctggcgcagc gtcggcaact
     1561 tcagccccca tacgctgtat tcagcaacgc cgtcaggaac acggctcact ttcaccttca
     1621 cgccgtcggt gacggactga acctccacgc tgaccggatt gccacttccg tcaaccaggc
     1681 ttatcagcgt ggtaccggag gatggcagcg tgatttcacg gtcgagcgtc agcgtccggg
     1741 tctggctgtt caccgccagc acgcgcccgc cggtgctgat accggcatag tcatcatcgc
     1801 agatttcaat gacatcgccc ggcacatggc gaagcccttc tgcgcccacg ctgaagtcca
     1861 cggtctgcgt ttccagcagt tctgttttaa tcagccacag cccggcgcgg tgtgcctgcc
     1921 cccggctggt acagccaaaa gcatccatct tcgtgacgtt acgaccgtaa cgggcaatgg
     1981 cctgcgtatc ctccacaagc tctgtcgccg tctcccagcc gttgttcggg tcaatccagt
     2041 tcacctcaac ggcattatgg cggtccttca gggcgctgaa gctgtagcgg aacggcgcgc
     2101 catcatccgg catcaccaca ttactgcggt tataggtcca caccttatcc gacggtcggt
     2161 cctgcacgaa cgtcagcgtc tgcccgttcc ataccggcat acagcgcatc gccgagcaga
     2221 aatcactgag cacatcccac gccttgcgct gtgtggtcag gtacgcatta caggtgatgc
     2281 gcagctccgt gccgccaaag ccgtccggca ctgactggtc gcagtactgg ccgatgacat
     2341 acagcgccca tttgtccaca tccgccgcac caagacgttt ccccatgccg tagcgcgggt
     2401 gggtcagcat atcccacaga caccaggcca tgttgttgct gtatgctggt tttaacgttc
     2461 cgtcccagat accgctgtat tgtcgcgtct gcgggttata gttcgacggc acctgcagaa
     2521 tgcgcccgcg aagatgataa ttacgactca cctgctggct gccgaactgc tccgagtcca
     2581 cctgtacgcc gaccagtgcc gtgttcgggt agcactgttt cacatcgatg atttcggtgt
     2641 atgacgacca gagcgttttg ttctgcagct ggtctgtggt gctgtccggc gtcatcctgc
     2701 gcatccggat attaaacggg cgcgg
//
LOCUS       DAZFWL020000289         2665 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFWL020000289 DAZFWL020000000
VERSION     DAZFWL020000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.292"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(89..1279)
                     /gene="aspC"
                     /locus_tag="ACHKNP_RS25525"
                     /old_locus_tag="pgaptmp_005105"
     CDS             complement(89..1279)
                     /gene="aspC"
                     /locus_tag="ACHKNP_RS25525"
                     /old_locus_tag="pgaptmp_005105"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HGU4707070.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMALLCEAI
                     VAVL"
     gene            complement(1464..2552)
                     /gene="ompF"
                     /locus_tag="ACHKNP_RS25530"
                     /old_locus_tag="pgaptmp_005106"
     CDS             complement(1464..2552)
                     /gene="ompF"
                     /locus_tag="ACHKNP_RS25530"
                     /old_locus_tag="pgaptmp_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HGU4707071.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          663 a          657 c          644 g          701 t
ORIGIN      
        1 aaggcctgat aagcgtagcg catcaggcaa tgttgcgttt gtcatcagtc tcagcccgct
       61 tttcagcggg cttcattgtt tttaatgctt acagcactgc cacaatcgct tcgcacagca
      121 gagccatgtt atctggtgtc atcccggcca catttacgcg accagaagca accgcatata
      181 cgccaaactc ttcgcgcaga cgcagcactt gttcttttgt caggccactg aaggagaaca
      241 tgccgttctg tttgatgata aagctgaagt cgcggtttgc gcctttttcc tgcagcgtat
      301 tgacgaacaa ctgacgcata cgctgaatac gctggcgcat atcagtcagc tcttgttccc
      361 aaatcgcacg taacgcatcg ttgctcagga tggtggcaac aacagaagcg ccgtgtgctg
      421 gtgggttaga gtagttagcg cgaatcgccg ctttcatttg gctgaatgcg cgatcaacgg
      481 tttcactgtc ggcagcaacc agagtacaag cgccaacacg ctcgttgtac aggccaaagt
      541 ttttagagta ggaactggca acaatcagct ctttatgcat agccgcgaaa gcgcgcagtc
      601 cttcagcatc ttcttccaga ccacgggcaa aaccctggta agcgaagtca aacagcggta
      661 accagccttt ctcaactgag agttgtgcca gtgtctgcca ttgttccagc gtagggtcga
      721 taccggttgg gttatggcag cagccatgga acagcactac gtcgccagcc tgagcttcgt
      781 tcaggctgtt aatcagtgca tcgaagtcaa gggtgtggtt ttctgcatca taataagcgt
      841 attcacgaac ttccagacct gcagagttaa agacgctctt atggttcggc cagcttgggt
      901 tgctcaccca cactcgctta acgctggtat tttttgccag gaaatcggca gctacgcgta
      961 gtgcgccagt gccccccgga gtctgtgccg tgcgagcacg tttgtcattg atcagggcgc
     1021 tacctttacc aaacagcagt tcctgagtgc agcgaccaaa ttcagggatg ccgtcaatgc
     1081 cgaggtaatt tttggtggtt tcattttcga gcagatactg ttcagccttt ttcacgctgg
     1141 tcaataccgg ggttttgccc gtctcatctt tatagacacc aatcccgagg ttaattttgc
     1201 cgggacgttc atcggcacga aacagatcgg ccaggcccag aatcgggtcg gcaggagcgg
     1261 cggtaatgtt ctcaaacatg acgaggttcc attatggtta cagaagggaa gtccgctatc
     1321 agggtaacgg gagatttaca aaattccaac tattactgat gaaaacgcag gctgtttttg
     1381 caagacgtga gattgctctg gaaggcataa aaaaaacagg accaaagtcc tgttttttcg
     1441 gcatttaaca aagaggtgtg ctattagaac tggtaaacga tacccacagc aacggtgtcg
     1501 tcagaaccta cgcccagttt gttgtcagaa tcgatctggt tgatgatgta gtcaacatag
     1561 gtggacatgt ttttgttgaa gtagtaggtt gcgcccactt caaagtagtt caccagatca
     1621 acatcaccga taccttctac gtctttcgct ttagatttgg tgtaagcgat ggacggacgc
     1681 agaccgaaat cgaactggta ttgcgcaact aacagaacgt cttgcgtttt gttggcgaag
     1741 ccgctggtgt ttgtaaattt attagtgatc ggcgtagcgt tacgggtttc accgtagttc
     1801 gctgccaggt agatgttgtt cgcgtcgtac ttcagaccag tagcccactg ttcagctttt
     1861 ttaccgttgc caagaggttg agcttcttgc aggttggtac ggtcagctgc accataagca
     1921 ccaacgatac caaagccttc gtattcgtag ctgatagaac cgccaacacc gtcgccgtta
     1981 gaacggcgtg cagtgtcacg ctcgttttta cccaggtact gaacagcgaa gttcaggcca
     2041 tcaaccagac caaagaagtt ggagttacga taggtagcaa cgccgccaac acgaccaacg
     2101 aagaagtcat cgctgtatgc agtatcacca ccaaattctg gcagcatatc ggtgtaaccc
     2161 agtgcatcat aaaccacacc gtagttacgg ccgtaatcga aagaaccaac gtcagcgtat
     2221 ttaagacccg cgaatgccag acgcgttttg ttaccagttt gagcgtcagc gccttcagag
     2281 ttgttaccct ggaagttata ttcccactga ccataaccgg tcagatcgga attgatttga
     2341 gtttcccctt taaaaccaag acgggcatag gtcatgtcgc cattgccacc gtaactgttt
     2401 tcaccgttac ccttggaaaa ataatgcaga ccaacagctt taccgtacag atctactttg
     2461 ttgccatctt tgttatagat ttctgcagcg tttgcagtac ctgctactaa cagagcaggg
     2521 acgatcactg ccagaatatt gcgcttcatc attatttatt accctcatgg ttttttttat
     2581 gacacctgcc actgccgtca ataagttctg tcaataaaaa tttacggaac tattgatgag
     2641 agtttggtgt ctttatgtgt ctgca
//
LOCUS       DAZFWL020000290         2606 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFWL020000290 DAZFWL020000000
VERSION     DAZFWL020000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.294"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1773)
                     /locus_tag="ACHKNP_RS25535"
                     /old_locus_tag="pgaptmp_005107"
     CDS             complement(<1..1773)
                     /locus_tag="ACHKNP_RS25535"
                     /old_locus_tag="pgaptmp_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4707072.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYVAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLTSLSAVLQKHVT
                     VDEVRPVLQAMVDDAVGAIPVPRDGRDYDPDVLQQAVNDAVANIPQPADGKSLTPDDV
                     RPMLEQMVKEAVSHIPVPRDGRDYD"
     gene            complement(1719..1940)
                     /locus_tag="ACHKNP_RS25540"
                     /old_locus_tag="pgaptmp_005108"
     CDS             complement(1719..1940)
                     /locus_tag="ACHKNP_RS25540"
                     /old_locus_tag="pgaptmp_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707073.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(1985..>2606)
                     /locus_tag="ACHKNP_RS25545"
                     /old_locus_tag="pgaptmp_005109"
     CDS             complement(1985..>2606)
                     /locus_tag="ACHKNP_RS25545"
                     /old_locus_tag="pgaptmp_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4707074.1"
                     /translation="RVHAQVSGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLT
                     EELIRFSSPAADALVRNALAEAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTG
                     NPDADAEAAFGQFVAANLQPTGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQ
                     GLPVIVSQYVGDQLVLVNAPDIYLADDGGVAVAVITGVNYGSASGG"
BASE COUNT          535 a          853 c          639 g          579 t
ORIGIN      
        1 atcgtagtca cgaccatcac gcggaacagg gatatggctt acagcctcct tcaccatctg
       61 ttcaagcatc ggacgcacat catccggggt gagactttta ccgtccgccg gctgcggaat
      121 atttgcgacc gcatcattca ccgcctgctg cagtacatcc ggatcataat cacgaccatc
      181 acgcggtacc ggaatggccc ccacagcgtc atccaccatc gcctgcagaa ccggacgcac
      241 ctcatccacc gtcacatgct tctgtaatac cgccgacagg gaagtcagtt tctcttcaaa
      301 cgcttgtgcc tgcgaggcca tcttcccctc aaatgtgcgc tgtaaatccg ccagcactgt
      361 ggagaattct tctcccagtg cacgaataat ggacagttcc cgttccgtca ttttctcagt
      421 atcccccctg aacatcgctt tcaccgcatc atgctctgtt tcactgattg ccttattacc
      481 gtcagatgcg ccgtcaggca gctgtgatga aactgttttc ccggcagacg cgaacggatc
      541 ctcacgggca tcacgacggg acagcgcctc cagactgtag ttctgctgct gaagatacag
      601 tgcatcaccg ccggccaggg gcggcaggtt ctcccgttta cgggcctcat tgggcgtgag
      661 aagcgtattt ttcaccgcat cccccagcgt tttcatgcgc cgctcactgt ccattctcag
      721 cagcgtggtg acatcaaatt ctgtactctc gttttccccc gtttccagcg cctcatccag
      781 taacagttca atggactcaa tcagcgtctg caggcactgg gaataatact gctgctccag
      841 cgcctccacg ttgtcactgg aaggcggttg tcccacgcca atcttgtagg ccgggacacg
      901 gaacaccgaa cagacaattt cagcggtcat cttcagttgt tccaccgtct gcgcatccac
      961 aggtgaaaac gtcgtggggt tgtattttgc cccgttgctc agaatggccg ttttccccgc
     1021 attttcgcct gtatacccgc tgtcccagtt gctcttcagt tttttcgcat tttcttccgt
     1081 aatactgccg gggatctcaa tcaccccgga cggcctgccg ccatttctga aaaaagacgt
     1141 cgaattttcc tgaatatgat gcccctgcgt ggccgccagc ccggcgacat acaccggcgg
     1201 caatcctata agcggatgaa aaaaacagtt aaaccggtcg tggatcactt cccgggcagg
     1261 caccgtcacc gcctccgtga tcccgcagtt ccggtccggc gtgatgcggt agaacacctc
     1321 gccgtcatcc gccaccagag gttcaacccg gctccagtcc agaatacgca gttctttgat
     1381 ctgcccccgg gcattacgga ttttcagcac caccgtattg ccatgacgca gtttggcgtt
     1441 cagccacagt tcaaaaaact ggatgcggtt ctgctgggcg ttgggacgac gacagaggcg
     1501 ggcaatatcc ccccggcgcg tttccctgcg tatcccatgc gcatccgtct gcataagacg
     1561 cagccgcatt ttggcgatat cctgggatat cagcgaaata catgcaaaca ccgcatgaaa
     1621 ggagaggacg gcttcaggat cggctttcac gccctgctgc caggcgccgg aaaagggctc
     1681 agccaccgcc tgaaacaggc tggtccagcc cgcctctctt acgtcacgtc ctgatttctg
     1741 gttttttcgg gttcgccgta aaaggttcca cattcgccat gctccgcatc acgtttcttt
     1801 ttctgacctg ccggacgtcg caccgtgatg tactccgcct ttcccaggcg aaccagcacc
     1861 tccgcacacg gctgtgccac atcacggata tccccggccc gggcatcatg cgtgccctgc
     1921 agatatcgga tctttgccat aacctgttac gggaggcgca cgcctcccgt cctccttatc
     1981 agactcagcc gccggacgca ctgccgtagt tcactccggt gatcaccgcc accgccacac
     2041 cgccgtcatc agccagataa atatccgggg cattcaccag caccagctgg tcacccacgt
     2101 actgggagac aatcaccggc agcccctgga aggagccacc cagcagggtc atgtccgggt
     2161 attccttctg acccagcgca tttttacgca tggacagtgc cagggcattg gtgctggaca
     2221 tcagccagac cgcaccggtg ggctgcaggt ttgctgccac aaactgtcca aacgcagcct
     2281 ctgcatccgc atccgggtta ccggttgatg ccgtgccctt cacatcatgg gtgatggacg
     2341 ccggggagac atctgccact gcggcttttt tcgggtccac aaagtctgta tccagacgcg
     2401 ccaccaccgc ttccgccagc gcattacgga ccagtgcatc agcagccgga ctggaaaaac
     2461 ggatcaattc ttccgtcagt accgcaatgg ccgacacctt cgcatgactg aaggtgatgg
     2521 attcaaaatc aaacttcgtc aggggttttg ccttaccctc acccacccag ccggcagcac
     2581 cgccggacac ctgggcgtgc acacgg
//
LOCUS       DAZFWL020000291         2602 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFWL020000291 DAZFWL020000000
VERSION     DAZFWL020000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.295"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(132..350)
                     /gene="ymgF"
                     /locus_tag="ACHKNP_RS25550"
                     /old_locus_tag="pgaptmp_005110"
     CDS             complement(132..350)
                     /gene="ymgF"
                     /locus_tag="ACHKNP_RS25550"
                     /old_locus_tag="pgaptmp_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HGU4707075.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(482..2005)
                     /gene="pdeG"
                     /locus_tag="ACHKNP_RS25555"
                     /old_locus_tag="pgaptmp_005111"
     CDS             complement(482..2005)
                     /gene="pdeG"
                     /locus_tag="ACHKNP_RS25555"
                     /old_locus_tag="pgaptmp_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4707076.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETNEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2337..2585)
                     /gene="ymgC"
                     /locus_tag="ACHKNP_RS25560"
                     /old_locus_tag="pgaptmp_005112"
     CDS             complement(2337..2585)
                     /gene="ymgC"
                     /locus_tag="ACHKNP_RS25560"
                     /old_locus_tag="pgaptmp_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HGU4707077.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT          801 a          566 c          538 g          697 t
ORIGIN      
        1 gacatataaa aacaactgtg ataacaccag ggttgacaac tatttgcgca ataattcctt
       61 tacttcaatg atccaggcat tcactgccag caacccattc agccgcatac tgacagtgag
      121 aaactgaaag attattgctg atccctgtgg ctgacgagtc tttcccttaa tcgattaacc
      181 agattgatcg ccagccaaac aagggcatta agcaaattta tcgtcacaag cacttctccc
      241 agcccggtac tgggttttgc aataaggatg accaggaggg tgatcagcat aaatgcaatg
      301 gaaaagatga tatagagagt gaaataatcc agattattac tattgttcat gctactaatg
      361 gcaatcctgt caaaactttc aaacaattat agcaaacaaa ataatgaaca atagtaacaa
      421 gaggcaacga gctaattagc tgtctataat tacaccgcac tatggcctta ctgatcaatt
      481 ttcactcaac cacaaccttc accttcggtt tagaaaggat aattttgacc aggtcgatgt
      541 atgtaacagg tttatagaaa taataaccct gctgaaatgt gatattattt tggttcagat
      601 agtcaagttg ttcattcgtt tcgacacctt cagcgacgat actgatcgaa agtttacgcg
      661 ccagatccaa tacacaatcc agaattcggg ttgattctgg gtctgcgttt acgcggccaa
      721 cgaaactgtg atcaattttg atataatcaa tatgcaggtc atgaagataa gaaagccctg
      781 agtagccagt accgaaatca tccagcgcga tgacaaaacc attttcatgc agaatgttca
      841 accgctgaac cagactttca tcaacattca atggctcacg ctcggtgact tccagaacaa
      901 ggtttaaatc gcggcgggta aaactgtcac ggaaatttaa acactcgtcg acaaacgtcg
      961 gcgaaataat atgcgaagcg ctaaaattaa ttccaatatg aaaaccttcc ggcaatttac
     1021 tcgcgatagc gttcatctgt ctggcaacct gattcatcag gctttgcgta agcggaacga
     1081 ttaatccgga tttttcagca agtggaataa atgccgcggg tgatatatat ccaccgtgag
     1141 gttgtttcca gcgggctaac acctcaactc cccgcaatgt cccttcccga ccatttacca
     1201 caggttggta aaaagggacg atctccccat tatttattgc ccgtcgcagg atctcttcag
     1261 gcgttgcgct tttattgaaa tatctatcaa gcaggaaggc agcggcgcag gcgattaaca
     1321 gaataaatat taaaatccca aagcctttat cgataagtct gtagaagcta aagagtggcg
     1381 gcgggttaaa gataatagtg aaatgatatt ttgtggagtg aaccttctcg gtatagggat
     1441 aatgtccatt aagcgtcatc acatcacccg ttggtccaat aatgtcatcc gccacgcgta
     1501 atacatacct tacccctttc aagggtacat taagtgcccc acgaatatgc tgatcgctta
     1561 tcgtaaccaa aatgcgcgta tctgcaaatt ggttctgata gagcaggata ggtaatctat
     1621 taacggtgtc gattgctgga gccaacagta aattactgtc cgggaaaaca ggaatacgag
     1681 acaacaggac ccgattccca ggcagggatg tacaccagac tattccctgt ttgagaatga
     1741 tgattgtgcg caggtgaggt ttcagagctg cttcagtgcc aagctgatat tgctcctcga
     1801 ggttgcattc cttcccggca atgttaaccg ctgtttgagt cgcttgtgac gcttcttcaa
     1861 gtatatgatt gatattgttg gcggcatatc tcgctcccgc caggtactct gcttttgcgg
     1921 aataccagag ctggatgttc aaaatagcga caccggtaat aaacaagcat atcgcaacga
     1981 ggatgggtat gagtgtattg cgcatttgct tgtcccggcc aaagggttta atacatgaaa
     2041 atgccattca ttcgctcgcg ctggcatcta cagagagcat ggtagagagc ataagaaata
     2101 tccacgccga agataaaatt ttctccatct tgatctaacc cattaattat tgagcataat
     2161 gtaggcagta caaaataagt taggcgggat atcaggcgtc aagaatggag agcgagaact
     2221 ctccattctt gacacctgat attgcggaca taataagaaa gcataacgcc tgaaatgctc
     2281 actttgcatc agcatggtga tacagctgat gtttattcta aaaccttact caagttctaa
     2341 gagagcacgg attccctgtc attaataatg atatccgtta gtgtagtctt atgcatttca
     2401 atgtactgca atatttgcag atagagatca gaaagaatca cactggtttc atcttttaaa
     2461 cgcaagcgta aagcctgatc aataatatga ctatcaacat acccatgcgt catgaacacc
     2521 tccctctcga gatttttaaa caaagcacat tgaaaaataa atctctctgg gattgaatta
     2581 ttcatgtcag cctcgtagaa at
//
LOCUS       DAZFWL020000292         2584 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFWL020000292 DAZFWL020000000
VERSION     DAZFWL020000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.296"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..344
                     /locus_tag="ACHKNP_RS25565"
                     /old_locus_tag="pgaptmp_005113"
     CDS             <1..344
                     /locus_tag="ACHKNP_RS25565"
                     /old_locus_tag="pgaptmp_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HGU4707078.1"
                     /translation="GLLQTMPYASNISIAVLDDNVLESQAIETLSAIGLSYEKYKTAN
                     NVYFNIIGTLSDSELNKINNYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFE
                     KSQGSNWTFQE"
     gene            358..598
                     /locus_tag="ACHKNP_RS25570"
                     /old_locus_tag="pgaptmp_005114"
                     /pseudo
     CDS             358..598
                     /locus_tag="ACHKNP_RS25570"
                     /old_locus_tag="pgaptmp_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            618..949
                     /gene="sctI"
                     /locus_tag="ACHKNP_RS25575"
                     /old_locus_tag="pgaptmp_005115"
                     /pseudo
     CDS             618..949
                     /gene="sctI"
                     /locus_tag="ACHKNP_RS25575"
                     /old_locus_tag="pgaptmp_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            946..1680
                     /locus_tag="ACHKNP_RS25580"
                     /old_locus_tag="pgaptmp_005116"
     CDS             946..1680
                     /locus_tag="ACHKNP_RS25580"
                     /old_locus_tag="pgaptmp_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HGU4707079.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSSASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            1696..2277
                     /locus_tag="ACHKNP_RS25585"
                     /old_locus_tag="pgaptmp_005117"
     CDS             1696..2277
                     /locus_tag="ACHKNP_RS25585"
                     /old_locus_tag="pgaptmp_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HGU4707080.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLASTLLERITFYAKKNRDELDKISCKWCCD"
     gene            2306..2491
                     /locus_tag="ACHKNP_RS25590"
                     /old_locus_tag="pgaptmp_005118"
     CDS             2306..2491
                     /locus_tag="ACHKNP_RS25590"
                     /old_locus_tag="pgaptmp_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707081.1"
                     /translation="MVSLKESILLETSAKIQSIEVKREEKFIQGYYDGYTKGIIDVMD
                     NFIPLISLLSSELEKKE"
BASE COUNT          892 a          404 c          492 g          796 t
ORIGIN      
        1 aaggattact gcaaactatg ccatatgcca gcaatataag tattgcggta ttagatgata
       61 atgtattgga aagtcaggct attgaaacgt tgtcagcgat aggtctttct tatgaaaaat
      121 ataaaacagc taacaatgtg tatttcaata tcattggtac gttaagtgac agtgaattaa
      181 ataaaattaa taactatgtt gacgaatatt ataaacaatg gggtaaacaa tatgtaagat
      241 ttaatgtgaa tttgaaaaat caggacacaa ataatagttc atttagctac ggagataacc
      301 gattcgagaa gtctcaaggt agcaactgga catttcagga ataattgagg aggacttatg
      361 gcagattgga atggttatat tatggatatc agcaatcaat ttgatcaggg cgttgatgat
      421 ctgaaccagc aagttgaaaa agcgttggag gatttagcca accaatccct ccgacacgaa
      481 atttcttgcc gaatatcaaa gtgcattagc tgagtataca ttatatcgaa atgcgcaatc
      541 taacgttgta aaagcgtata aagatcttga ttcagcaatc atacaaaact tccgctaaac
      601 tagataagga ttttattatg ttagtaagca atatgccacc tattgataga gctgagcaat
      661 caactgctca tgaaatacag caagcgaaag tgattgattt aaatgaccgg gttctcaatc
      721 ttgacaaccc ggatgataaa atgatctccg cttttgccaa ctatgctgta caaactgaaa
      781 attggcagca aaatgcgtta caagcattaa ggtctgataa agaggggtta actcctgaaa
      841 aattattagt cttgcaggat catgtgctta attataatgt cgaggtttca ttggtggaac
      901 attagcaaga aagattgtcg ctgcagttga gacgttgacc cgctcatgaa atatatatca
      961 ttattattgt ttatcctgct gttgtgcggc tgcaagcagc aggaattatt gaaccatctt
     1021 gatcaacagc aagctaatga tgtccttgct gttttacaaa gacataatat taatgctgaa
     1081 aagaaggatc aaggcaaaac aggattttca atatttgtcg aaccaacgga ttttgcttct
     1141 gcggtggatt ggctaaaaat ctacaatcta ccgggtaagc ctgatattca aatatcgcag
     1201 atgttccctg cagatgcact cgtttcatct ccacgagcgg aaaaagcaag gctatattct
     1261 gctattgagc agcggttaga gcaatctctc aaaattatgg atggcattat ttcttcgaga
     1321 gtacatgtta gttacgatgt tgataatggc gatagtggta aaactgcttt accaattcat
     1381 atttctgtac ttgcagtata tgaaaaagat atcaacccag aaataaaaat aaatgatatc
     1441 aagagattta ttgttaatag ctctgcaagc gtacaatatg aaaatatttc tgttgtattg
     1501 tcaaaacgaa gagacattat tgagcaagca ccaacatatg aaattagcga acctgttttt
     1561 gcttatgata aggctatgcc ggtttctatc ttacttgcat taatatccgt agcgacttgc
     1621 tggctgttat ggaagtatag ggccattcta actaatttag tcaggttgaa aattaagtag
     1681 acatatgaga gcgttatgaa tcttgcccta agaaagatta tatatgatcc aattagttat
     1741 attcatcctc aacgagtaag cttgaataat acacctatta acaatccggt gcttagatct
     1801 attaccaatg aaatgattgt tctgcagtac aatctttcag ttgaacattt taatctgaat
     1861 agttcactta tttactacat aaacaattgg aatttatttc cattgttctg tttattcagt
     1921 ggttatcact tttatcgaga aaggtttgca gaacgcggat ttttttataa ggttcctgct
     1981 gtgctaaggg attatttatc agctataccg gtgaaaataa atgaaaaggc acgatacaaa
     2041 cccggcatcg ctagctatca caatatcatc acatgtggtt tttcaacgtt gtcaccctat
     2101 ataaggcaac aaccattggc aatgcagcaa cgatttaatt tattatttcc tgattttgtt
     2161 gatcacatac aattgccgtt accacttgca tcaactcttt tagagagaat tacattttat
     2221 gcgaaaaaaa atagagatga gcttgataaa atctcctgca aatggtgttg tgattaaaag
     2281 aaaaatttct gatggattga aataaatagt ttctcttaaa gaaagtatcc ttcttgaaac
     2341 cagcgcaaaa attcagagca ttgaggtaaa gagagaagaa aaatttattc aaggttatta
     2401 tgatggctat acaaaaggca ttattgatgt gatggataat tttataccct tgataagctt
     2461 gttgagctca gaactagaaa aaaaagaata aacatgataa acgatctgaa gagtatattg
     2521 ttaaaatcct cagaggaagt tgatgttttt atcaaaattt ttgaaagttg gagaaaaaaa
     2581 ttac
//
LOCUS       DAZFWL020000293         2565 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFWL020000293 DAZFWL020000000
VERSION     DAZFWL020000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.297"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            185..484
                     /locus_tag="ACHKNP_RS25595"
                     /old_locus_tag="pgaptmp_005119"
     CDS             185..484
                     /locus_tag="ACHKNP_RS25595"
                     /old_locus_tag="pgaptmp_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707082.1"
                     /translation="MALYIDISAIAGQVRIIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IKLRGLIFSYKVTTIFADGSRETVRAMRFKGCVKDFATTFWARKLDCIHNQFPLE"
     gene            556..774
                     /gene="ydfC"
                     /locus_tag="ACHKNP_RS25600"
                     /old_locus_tag="pgaptmp_005120"
     CDS             556..774
                     /gene="ydfC"
                     /locus_tag="ACHKNP_RS25600"
                     /old_locus_tag="pgaptmp_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGU4707083.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            1343..1531
                     /gene="dicB"
                     /locus_tag="ACHKNP_RS25605"
                     /old_locus_tag="pgaptmp_005121"
     CDS             1343..1531
                     /gene="dicB"
                     /locus_tag="ACHKNP_RS25605"
                     /old_locus_tag="pgaptmp_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4707084.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            1528..1716
                     /locus_tag="ACHKNP_RS25610"
                     /old_locus_tag="pgaptmp_005122"
     CDS             1528..1716
                     /locus_tag="ACHKNP_RS25610"
                     /old_locus_tag="pgaptmp_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4707085.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            1811..>2565
                     /locus_tag="ACHKNP_RS25615"
                     /old_locus_tag="pgaptmp_005123"
     CDS             1811..>2565
                     /locus_tag="ACHKNP_RS25615"
                     /old_locus_tag="pgaptmp_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4707086.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPI
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPVIAAIHREYKQTWKTLD"
BASE COUNT          729 a          583 c          647 g          606 t
ORIGIN      
        1 cggcagtggt caggggttaa gtagccagcc tgaggcgtat gaacatgacg gcggggatta
       61 cgtttaacta tgcagcaggt tttttgttcc gctaccccgg cgttaagggg aaacagagga
      121 tttctcagtg ggcgaagtca aacatcagaa tggaaggcat ccagggatca gcaaagaaac
      181 agcgatggcg ctttatattg atatcagcgc cattgccgga caggtaagaa ttatcagagc
      241 ggtaactaag cggtatgcgc ctttacttca gaaagtctct ggtgagtgca ccgaagatat
      301 tgtcaacgat ttcgtcatca aactgcgagg actcatcttc agttacaagg tgaccacaat
      361 ttttgcagat ggctcccgcg aaactgtcag agccatgcgg tttaaaggat gtgtcaaaga
      421 cttcgccacc acattctggg caagaaaact tgattgtatt cataaccaat ttcctctcga
      481 gtaacagacc cctcagagga taccacctcg cctgacgtgg ttaaaagcag gcagcgctaa
      541 ccacaaggag ccgacatgca gaaacgagaa cccgtcatca tcgcgccaga ctataccgat
      601 gatgaacttt atgagtggat gcaccagaaa attaaggcag cgcaggacct gaaatgggcc
      661 aatgaagcca gggctaagca ggctgaaaat ctgtccgctc tggagcagga tatcaccaat
      721 ctggaaaaag cagcggcatt aagcattgcc agaatgatta catacccgcg ttaatagcta
      781 accaacgaag ctaaggttgg taattaagga gttctccacg ggtgaggtgg agtgcgtgcg
      841 ccggacacgg gtgagcatcc ggcactgaca gtttactgaa aggatatttc cctgaaaagt
      901 cagaccataa cgcgaaagcg cacggcgagg tagctggttc atagatagcc tgtcgttaaa
      961 ttttcgtcga ccgtgcgctt ccggttgtgg caatccgcga aatggcgcgg cggtaagtat
     1021 ggcggggtta ttccttcccc agttgaggac accgggttgt caggttgacc atacgcttaa
     1081 gtgacaaccc cgctgcaacg ccctctgtta tcaattttct ggtgacgttt ggcggtatca
     1141 gttttactcc gtggctgctc tgccgccctt tttaaagtga attttgtgat gcggtgaatg
     1201 cggctcagcg cacgcggaac agttaaaacc aaaaacagtg ttatgggtgg attctctgta
     1261 tccggcgtta attgttaact ggttaacgtc acctggaggc accaggcacc gcatcacaaa
     1321 attcattgtt gaggacgcga taatggaaac gttattacca aacgttaata cgtctgaagg
     1381 ttgttttgat attggtgttc tgctcagtaa ccgggagttt actgaagatg ccattaatat
     1441 gaggaaatat gagccttatc tgctcaatga taattccata ctttcccgaa ttgctcttct
     1501 tgaacttggt attttcggag aacgtcaata acttcagcat ttgcactgat gatgacggtt
     1561 tttcttataa cgggtgaatc acagaatgtg attaccggaa tttatgccag taaagaatcc
     1621 tgcctccagg caagagacga gcaaaaaatt tctggtgaat gcctcccggt aaaaaaagta
     1681 tcgctgtacc tgaataacga aacaccggct ggataaccct ccagccatat taacaccata
     1741 ccaacggatt aaaaatgcca gcaatggcag ggattcgttc accctgaaat ctgtaatgag
     1801 gtaaaacaaa atgagtaaag tctttatttg cgccgccatt ccggacgaac aggcaataaa
     1861 ggaagaaggt gcagtcgctg tagccactgc cattgaagcc ggtgatgaac gtcgcgcccg
     1921 cgcaaaattt cactggcaat tcctggaaca ttatccggct gctcaggact gcgcttataa
     1981 atttcttgtt tgcgaggata aacccggtat tccccgccct gccctcgatt cctgggatgc
     2041 tgaatatatg caggaaaacc gctgggatga ggagtctgct tcctttgtcc cggttgagac
     2101 tgaatcagat ccgataaacg tcacttttga caagctggcc cctgaagtac agaacgctgt
     2161 catggttaag ttcgacacat gtgaaaacat caccgttgat atggtgatta gcgcgcagga
     2221 actgttgcag gaagacatgg caacattcga cggacatatc gttgaagcgt tgatgaaaat
     2281 gccagaagtt aacgccatgt atccggagct taagctgcat gccatcgggt gggttaagca
     2341 taaatgtaag ccaggtgcca aatggcccga aattcaggca gagatgcgca tctggaaaaa
     2401 acgtcgcgaa ggtgaacgca aggaaaccgg aaaatacacg tctgttgttg atctcgcccg
     2461 cgccagagcc aatcaacagc acactgaaaa ttcaacagga aaaatcagcc cggtcattgc
     2521 tgccattcat cgcgaataca agcagacatg gaaaacactg gatga
//
LOCUS       DAZFWL020000294         2545 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFWL020000294 DAZFWL020000000
VERSION     DAZFWL020000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.298"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            235..600
                     /locus_tag="ACHKNP_RS25620"
                     /old_locus_tag="pgaptmp_005124"
     CDS             235..600
                     /locus_tag="ACHKNP_RS25620"
                     /old_locus_tag="pgaptmp_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HGU4707087.1"
                     /translation="MSDMTISLPKEVNGHVICSTRIRHILAIRKSILMQIYMLFRQQA
                     GISALRQELEVKTPYRAMNHPVIGVVTKADLASMEHISLVKCWLREAGAHNVLVTSAV
                     NNNGVTELFALLHTEEGCC"
     gene            complement(701..1030)
                     /gene="tmaR"
                     /locus_tag="ACHKNP_RS25625"
                     /old_locus_tag="pgaptmp_005125"
     CDS             complement(701..1030)
                     /gene="tmaR"
                     /locus_tag="ACHKNP_RS25625"
                     /old_locus_tag="pgaptmp_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HGU4707088.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1202..2260)
                     /gene="yeeA"
                     /locus_tag="ACHKNP_RS25630"
                     /old_locus_tag="pgaptmp_005126"
     CDS             complement(1202..2260)
                     /gene="yeeA"
                     /locus_tag="ACHKNP_RS25630"
                     /old_locus_tag="pgaptmp_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU4707089.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLIDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(2458..>2545)
                     /locus_tag="ACHKNP_RS25635"
                     /old_locus_tag="pgaptmp_005127"
     CDS             complement(2458..>2545)
                     /locus_tag="ACHKNP_RS25635"
                     /old_locus_tag="pgaptmp_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase inhibitor"
                     /protein_id="HGU4707090.1"
                     /translation="CFEVYLNNGAEDGYWDIEMYVAVQPKHH"
BASE COUNT          705 a          614 c          589 g          637 t
ORIGIN      
        1 cctactgatt gctaaggata aacaagtatt aaaggccaga ctgataagac cgcgactaag
       61 tgaacctgta tctgacatgg catagcttcg ctaatgtgtc atcatgatta ggactaaatc
      121 tgtgaattcc tttaggaagc acggacacag gccaatctga gtccatatgt atgattggaa
      181 tcttacacag atgacttttt gacacgggga cttggcaaac aggggcgacc atgtatgtca
      241 gatatgacaa tctcacttcc gaaagaagtc aacgggcacg ttatctgttc aaccagaatc
      301 cgacacattc tggctatacg aaagagcata cttatgcaaa tctatatgct tttccggcaa
      361 caagcaggaa tctcagcgct gaggcaggaa ttagaggtaa aaacgcccta ccgggccatg
      421 aatcacccgg tcattggagt agtaaccaaa gcagatttag ccagcatgga acacatatct
      481 ttggtgaaat gttggttacg ggaagctgga gcgcacaacg tgttagtaac cagtgcggtt
      541 aataacaatg gagttacaga actttttgcc ttgctgcata cagaagaagg ctgttgctaa
      601 tttcgatagg ttctcagatg agaatgatga cggtgataca cagataagaa tcaatgagca
      661 atgagggttg ccgggcaacc ctcattgaat aaaacaggaa ttacttcgct tcgccgtttt
      721 tcatttcgcc catagctttc agctttttgg agatatcgcg gcgttctttg gagagctcgg
      781 catttttgat gatgtaatcg tcaacgcgat cttcatagtc acctttcatg ctggcgatga
      841 tgccctggat tgcttcaacg ctcatcccgg gcttgatgta atcgctcagg ttgtccagca
      901 acaggacgcg cttctggttg tcacggatct ttttctcaac gtcctgaatt tcacgttgca
      961 gtttgttctt acgacggaac agacgaacaa attccagtac gtcctggaat gaaggcttgg
     1021 tagtttccat ttttataccc ctgataatgt gagagtcgga ttcgtttaag caaccgctat
     1081 tcgttagggc caacattact catagctgcc gcagatgaca atgcttttat cccttttcac
     1141 tatcataccc tttatccttg ctgaatcgaa gcagcagcaa gatgattctg aagttcagga
     1201 attatttgcg caaggcccgg caaatcagac tcgatagcaa ctcaagctga tgtgccgttt
     1261 ccatgttcag ccacacataa ccatagattg gtgtttccac aaccttcagg tcatggtggt
     1321 tattcagcaa ctgacgcagc tcttccacag catcgatcaa tttttccgta ttggcaaaaa
     1381 ccggctgcgg attaccttcg tacagcgcat gaacaaggct cagcagtatt tgctgcatca
     1441 tgtgctgggt atcacgaagt ttttgcgcgt tcaataacac gaaatggctg gggcgcgtgg
     1501 cccagtatgc attgatttgc aactccagca tacaaaccag attgcggtta atggtctgga
     1561 taccttcata tatcgatttt ggaatacgag tttctttgct ggcgggcgca atcagcccac
     1621 gcattttcac ggcatcggtc aggagttttt gtagatggct ttccagacgt gggcgttcga
     1681 gtaagttcgg tgagaatgca gattgataaa cccgattata ctcggtcaga cttttcgcca
     1741 gttgaatgcg ccagtggatg aacgcccgtt gtggccagat accggtaaac aacattgcca
     1801 gtaaagagcc gaggatcaca tcgccgcttc gccataacgc cgtatcaatt tcacctgtcg
     1861 gggaacccac aacaattgcc agcgtcaccc caatcaataa tccttgatac ggtttcttgc
     1921 ccagcgccag ccaaccgcaa agaaacatag ccgccgcgca ccagactaac atcagcggta
     1981 acgaaattaa ctccagttgc agagcgataa gacctaaaat cgaacccaac accgtaccgc
     2041 caatacgttc gaaggcgcga gggacaacgt taccccagaa cgagattggc cccataatca
     2101 ccaccatggt gaccagcggc caggtgcttt ccgggatagt aaacaggcgg ataatgagaa
     2161 aagtgagcag gaatgccagc gcgacccgag taccatgcac aatgcggtaa tggcggtata
     2221 cccggatttc aaacgggctt aatgacttat cggcacgcac gcagaaactc cgtaatgaag
     2281 attaaaagtg ctcataactc atctgtcggc cagaaggccc gcctctgaat tatccgacgg
     2341 acaaaccggg aacagtgtaa tttcccggca ggagcgaaaa aattgaaagg cgcatcttac
     2401 tcttttcgct ttcaaaaaaa gagtggtcat cgcgttaaca caccgccctg agatgaatta
     2461 gtgatgtttt ggctgcaccg caacatacat ttcgatatcc cagtacccat cttccgcgcc
     2521 attgttcaaa taaacctcga agcag
//
LOCUS       DAZFWL020000295         2509 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFWL020000295 DAZFWL020000000
VERSION     DAZFWL020000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.299"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..388
                     /locus_tag="ACHKNP_RS25640"
                     /old_locus_tag="pgaptmp_005128"
     CDS             <1..388
                     /locus_tag="ACHKNP_RS25640"
                     /old_locus_tag="pgaptmp_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707091.1"
                     /translation="RHIRKMQKKLKQTSVPDFIYYSIEKHNKYPIYVLHMPSNNIIEI
                     GYNIINTDLVIGEKIHFRTLSNRSLYFNLTQPPLIATIKDDVFCTLHDYYNHNNETKS
                     TIDNYISKIKDNHNTPWLLNNENVQE"
     gene            complement(1259..1399)
                     /locus_tag="ACHKNP_RS25645"
                     /old_locus_tag="pgaptmp_005129"
     CDS             complement(1259..1399)
                     /locus_tag="ACHKNP_RS25645"
                     /old_locus_tag="pgaptmp_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4707092.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(1701..1964)
                     /locus_tag="ACHKNP_RS25650"
                     /old_locus_tag="pgaptmp_005130"
     CDS             complement(1701..1964)
                     /locus_tag="ACHKNP_RS25650"
                     /old_locus_tag="pgaptmp_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU4707093.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            2387..>2509
                     /locus_tag="ACHKNP_RS25655"
                     /old_locus_tag="pgaptmp_005131"
     CDS             2387..>2509
                     /locus_tag="ACHKNP_RS25655"
                     /old_locus_tag="pgaptmp_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HGU4707094.1"
                     /translation="MKTKKVMMIALVSSALALSGCGAMGTAIKKRNLEVKTQMSE"
BASE COUNT          795 a          574 c          451 g          689 t
ORIGIN      
        1 acgacatata agaaagatgc aaaaaaaact aaaacagacg tctgtgcctg attttattta
       61 ttattcaata gaaaaacaca acaaatatcc catatatgta ttacacatgc catcaaacaa
      121 tataattgaa ataggctaca atataataaa caccgatctg gtcattggcg aaaaaattca
      181 tttcaggaca ctaagcaatc gttctttata ttttaacctc acccaacccc cattaatcgc
      241 cacaataaaa gatgatgtgt tttgcactct tcatgattac tataaccaca ataatgaaac
      301 gaaatccact attgacaatt acatatcaaa aataaaggat aatcataata ccccatggtt
      361 attaaacaac gaaaatgttc aggaataaaa aaaagagaca attcccgaga taaaacaaaa
      421 gagcataaac atatgaaccg gaaattctat ttcaaaaata tcctgtgggg aatgtgcccc
      481 attataatat ccatattgca tctgatatat gacacaccaa cagaagcatc gataaaagtt
      541 gacttatttc tgatactcag tggcctggcc cttccttgtt caatatacct gacaaaaaca
      601 atcacactaa aatacacatc catatcattc tggaaaaaac ctcttttctg gtcttatggt
      661 atatatcatt ttcatcttgt ttatataatg acatgtatag ttttagcaat accttctgca
      721 attatcgttt tattctttca cttttatcaa aacatcacca gaaactggaa gtgacaaccg
      781 caataatgaa cataaaaagg caaatcagaa tggcagtaaa tcaataaagt accatttcat
      841 atgaatcatc agaaaaacgg tggacgcgac atccagcacg aggcaaaaca cagcaacaaa
      901 ataagccgga tatcgcgccc tgtacttttc taactcaact cccggcattg tgcaagtttt
      961 aatcgcaggt ggattcagag ccacgccccc attgattcag cctgaagaca cgtatatcct
     1021 tggagaaagc aggaatctca ttgctgtgaa attccttaca gaatcttcag ccccatttta
     1081 tggggctttt tttggtgccc ccacgctgtt accacacttt cgggatgcga ataaaaaagc
     1141 ccacagtttt tacactgcgg acttttgttt tttctttgtg atttgtgtgc aagggtgcga
     1201 ctttcgaccg aacctttggc aaccggatag cggggactgt tgtccccgct gcggtttctt
     1261 acggtttaca ctgcaagaac gccgcaattt ccgaccacat gaagcttacc ctgaattcac
     1321 acggtgatgc gtgtaacacc cagacggtca gcaacatggt caggcataac atgcccacaa
     1381 tgatcgtttt ttgcggcata gcacttgctc cttttacaga gaggcgctaa ccttccactt
     1441 gctgaaggta gatggtcagg gcctcgggtt aacgtttaag ttgactcggg gcctttccac
     1501 atccggcctt caggtaatcc ttccagccgt cagccgaaag gcacccgcgc gtaatgtact
     1561 ggttttcaga gaggtccgca atcttccggt gacgagatca tctgcacacg ccccggtggg
     1621 acgggctaac ggtatattca ggtgcaggat ctctctgtct ggtgcgtttt taaattgtca
     1681 aacgcaccct ggcggcaaaa tcagccacct tttttcgcat caacaaaacg cccaaaacgc
     1741 tgggtaaagc gtgcaacatt accttctgaa gcgacagttc tcagtttacc tgtataaaac
     1801 ggatgcgact tagaagaaac gtcaattgta acatatgggt aagttacacc atccagttca
     1861 attacccggt cagttttgat cgtcgaaccg atcctgaagt actcatcaat actggtatcg
     1921 tgaaatacta ctgtacggta ttccggatgg atattcggtt tcacaacagt tttccccctt
     1981 taagtgaaaa ttccgcataa aggtacatat tcagacggtc agattcaact gtctgttctt
     2041 ataacctcac tctctttcag tattacaata tgttatataa ttataaactg gacttcccga
     2101 aaaaatccat taaataagaa gcgagccgtt gcggaggctt gctctctcct ttaacctcat
     2161 gtgcaatatc cgtaacgcgc tcacataaat catcatctga taatataaca acacccatta
     2221 ttttattctg acctaaaaac ttcttatgaa ataaacgttt acccgacgta tctgatcccg
     2281 ctgtttttac ctgtatcctt taacatactt gagaagcccg ctgaaaaata taccgtaaag
     2341 gcggttctct gtcttccata aacctgtcgg ataagaaggg cattaaatga aaaccaaaaa
     2401 agttatgatg attgcactgg tcagctctgc gctggctctt tcaggatgtg gagccatggg
     2461 cacagcgatc aaaaaacgaa atctggaagt aaaaacccag atgagtgaa
//
LOCUS       DAZFWL020000296         2455 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFWL020000296 DAZFWL020000000
VERSION     DAZFWL020000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.300"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /locus_tag="ACHKNP_RS25660"
                     /old_locus_tag="pgaptmp_005132"
     CDS             <1..198
                     /locus_tag="ACHKNP_RS25660"
                     /old_locus_tag="pgaptmp_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4707095.1"
                     /translation="SLTAQLRLGPADILESDENGIIPEQDRVITQVVILDADKKQIQC
                     VVRPLQILRADGTWENIGGMK"
     gene            311..886
                     /locus_tag="ACHKNP_RS25665"
                     /old_locus_tag="pgaptmp_005133"
                     /pseudo
     CDS             311..886
                     /locus_tag="ACHKNP_RS25665"
                     /old_locus_tag="pgaptmp_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
     gene            1177..1758
                     /locus_tag="ACHKNP_RS25670"
                     /old_locus_tag="pgaptmp_005134"
     CDS             1177..1758
                     /locus_tag="ACHKNP_RS25670"
                     /old_locus_tag="pgaptmp_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU4707096.1"
                     /translation="MPGLVSYISSTSFANEMAEMRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGLGLVYAELLNIGFNDGVDALRNRVLSVLPGMVAQRQENSLQAKISE
                     CTFTVDIEKLHCPGEVLQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLTGEGLP
                     HPLTREPITASIIVKHEECIYDDTRGNFVIKGN"
     gene            1826..>2455
                     /locus_tag="ACHKNP_RS25675"
                     /old_locus_tag="pgaptmp_005135"
     CDS             1826..>2455
                     /locus_tag="ACHKNP_RS25675"
                     /old_locus_tag="pgaptmp_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGU4707097.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRMERRAVALERQLNGGVDFLSSVNNYFQSVMA
                     EHRENKTSNKILMEKINSCVFRPDSNHFSCPESFLTCPITLDTPENGVFMRNSRGAEI
                     CSLYDKDALVQLVETGGAHPLSREPITESMIMRKDECHFDSKKEAFVASD"
BASE COUNT          714 a          418 c          630 g          693 t
ORIGIN      
        1 tcgcttacag cacaactgcg actggggccg gcagacatcc tggagtccga tgagaatggt
       61 attatcccgg agcaggacag ggtaatcacg caggtggtga tactggatgc ggataaaaag
      121 cagatacagt gtgtggtaag accgctgcaa attctgcgtg ctgacgggac gtgggaaaat
      181 attggcggga tgaagtaacc cgacagcttc acaaaaccgg agtccggctc cggtttttgt
      241 tgtcatgtat gggggctgtt tgttatgact ccctgtgttt ggaatgaata tttaaatagg
      301 gagttttgtc atgccgttaa catcagctat tgcatccaat tcattttcca ccggaatgca
      361 ggttcttcgt gctcaaatgg ccgccagtgg cggtggagaa attacagtag gcgggtagac
      421 ggtccgtatc acatatagtg aaacggatgg tcgctttctg gcgagtgggg gcaataacag
      481 tttgctttct ggattattac ttacagggct taatggtggt cctgaagcgc tcagggatat
      541 aatgttaaga atggtttcag gttcaggtaa cacacaatca catggtgata ttgaggggaa
      601 aatatcacaa tgtaagtttt ctgttaatac ggagagcctt cagtgtccat ccgaggcggt
      661 tcgatgccca attatactgg ataaaccaga agaaggtgtg tttgttaaaa attcagaagg
      721 ttctttggtt tgtaccttat ttgattcggt ttctttttct catttggttc gcgacggtgg
      781 gaagcacccg ctaacacgag aaccaataac gtcatcaatg attgtaagtc aagaacaatg
      841 tatttatgac caaaccaaag gaaactttgt cataaaggat aagtgaaata aatattaccc
      901 aagctatatg ttaactgcca gttgcttata tgaaatgcta cagatgttca gggtataagg
      961 atgtggtaat tggtgtactg gatgcagcta aaaagcatat acagtgagtg gtgaggccgc
     1021 tgcaaatctg gcgtgctgac ggggaaatat tggcgggatg aaatagccga cggattcaca
     1081 aaaaccggag tccggctccg gtttttgttg tcatgtcagg gagatgtttg ttatgaagcc
     1141 cagaggaaat atttatctgt atgaaggaat atggtaatgc ctggattagt atcatatata
     1201 tcatcgactt cattcgcgaa tgagatggcg gaaatgcgtc agcaggtaat ggaagggcag
     1261 attggaggat ttctcctggg aggggagaga gttagagttt cttatatgcc agatacaggc
     1321 cgttttttag cagaaagtga agggctggga ctggtttatg cagaattatt gaatattggt
     1381 tttaatgatg gagttgatgc gctcagaaac agggtgttaa gcgtgcttcc tggaatggtg
     1441 gcgcagcgac aagagaactc tttgcaggcc aaaatatcgg aatgtacctt tactgttgat
     1501 attgaaaaac ttcactgtcc tggtgaggtg cttcaatgtc caattacact ggagcagcct
     1561 gaaaaaggta tttttgtgaa gaattcagat ggttcagatg tatgtacttt atttgatgcc
     1621 gctgcatttt ctcgtttgac tggtgaaggc ttaccccacc cactgacccg ggaaccaata
     1681 acggcatcaa taattgtaaa acatgaagaa tgcatttatg acgataccag aggaaacttc
     1741 gttataaagg gtaattgaaa tgaacattac cctttattta atgaaacatc ctgcaaactg
     1801 atatgaatta ctgaatgagg tttttatgcc tgttaccacc ttaagtatcc caagtatatc
     1861 tcaattatct cctgcaggag tgcagtcttt gcaggatgca gccagacttg aaagtggaat
     1921 aagaatatcc attggtagtg gccaatattc tgttcactat gtccaactac tggatggatt
     1981 ttcagttgaa ccggtgagag gaggcttact ggataggcta ttggggcgtg agcatcgaat
     2041 ggagagaagg gctgtggctc tggaaaggca attaaatgga ggtgtcgatt ttttaagtag
     2101 tgttaataac tattttcaga gtgtcatggc agaacacaga gaaaataaaa caagtaataa
     2161 aatattaatg gaaaaaataa attcttgtgt atttagaccg gattctaatc acttttcttg
     2221 cccggagtca tttttgacat gcccgataac gctggacaca cctgagaatg gagtgttcat
     2281 gagaaactca cgaggtgctg agatatgctc tctatatgat aaggacgcgt tagtgcaact
     2341 tgttgaaact ggtggagctc atcctctgag tcgagaacct ataacagaat caatgatcat
     2401 gagaaaagac gaatgtcact ttgattcaaa aaaagaagcc tttgttgcaa gtgat
//
LOCUS       DAZFWL020000297         2444 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFWL020000297 DAZFWL020000000
VERSION     DAZFWL020000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.301"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(28..213)
                     /locus_tag="ACHKNP_RS25680"
                     /old_locus_tag="pgaptmp_005136"
     CDS             complement(28..213)
                     /locus_tag="ACHKNP_RS25680"
                     /old_locus_tag="pgaptmp_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4707098.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            complement(435..548)
                     /locus_tag="ACHKNP_RS25685"
                     /old_locus_tag="pgaptmp_005137"
     CDS             complement(435..548)
                     /locus_tag="ACHKNP_RS25685"
                     /old_locus_tag="pgaptmp_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707099.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            complement(769..1302)
                     /locus_tag="ACHKNP_RS25690"
                     /old_locus_tag="pgaptmp_005138"
     CDS             complement(769..1302)
                     /locus_tag="ACHKNP_RS25690"
                     /old_locus_tag="pgaptmp_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4707100.1"
                     /translation="MHEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1462..1734
                     /locus_tag="ACHKNP_RS25695"
                     /old_locus_tag="pgaptmp_005139"
     CDS             1462..1734
                     /locus_tag="ACHKNP_RS25695"
                     /old_locus_tag="pgaptmp_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707101.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            complement(1990..2196)
                     /locus_tag="ACHKNP_RS25700"
                     /old_locus_tag="pgaptmp_005140"
     CDS             complement(1990..2196)
                     /locus_tag="ACHKNP_RS25700"
                     /old_locus_tag="pgaptmp_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4707102.1"
                     /translation="MEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
BASE COUNT          647 a          673 c          499 g          625 t
ORIGIN      
        1 ctccttccag ttgcttctgc attgccatca gccgttctct gaggatgaaa taatcccgtt
       61 cagcggtgtc tgccagtcgg gggccggttg cattatccac gccggaggtg ccggtggctt
      121 cacgcacggt accggagcag gtggcgttga tccgcaggcg cttacgacca gcggcaacat
      181 cagcacgcag agtttcattt tcagctctcg catcggctaa ttccctcgag tatctggcat
      241 caagtgcagc gacatcacgc tggcgtatct gcatatcagt aattgtcgcg ttcgccagct
      301 ccagctcact ggctttttta tcgcgctgct ctttgtagat gatggcgtga tcacggtaat
      361 gattcagccc cagactaagc gcaccacagg ccaccagcag gacaatgata accacgcaca
      421 gaacacggtt catatcacca ccaacggatt gcccagacca gaacagcaat ggccacaata
      481 cgaatggcaa aagctgccgc tcttgttaaa tccagactgg ctggcgtctc cacttcaatg
      541 cctttcataa tggacaacct cagaaagaat cttttatact tcctcacagg gaaagtacct
      601 ccctacccat aatttctccc ttgccttgct caaggtcaga aaacacaaaa ccccgcttgc
      661 tgccaacaaa cggggttttt acttttattc acttaggttt taccagtttt caggatttcg
      721 tgttatccac ccgcgttggc caacgtcatt tttcaggaaa atattctgct atctgtcgat
      781 gtcccagcac gccagcgcgc tctcctggtc acgccgtgag acctgaccgt agcaattatt
      841 tgaacggata cggcagtctc tgccaccgtc cttaatccac cagcgaatcg cctcacaggc
      901 accttttcga tcgcctgcat taattcgttt ataaaacgtc gacgggaagc acttaccggg
      961 gccaatgttg tacggacaga atgacgcgat ccccgctttc tggggttcgg tcagcggcac
     1021 ccggatgttt ttctccaccc atgccagcgc cttgtcacgt tcgatggcat taacccggtc
     1081 gcatttttcc tttgacagct tcatgccagg aataacaggc ttaccatcca ccagaatggc
     1141 accacggcag atggtccaga tccccgcacc atcacggtat gccgtggtgt ggttaccttc
     1201 cttttcatcc agaaactggt cgaggatttc aggcgcagaa gcacctgcac caatcagcgc
     1261 cagaacggca gccgacaggc cgtattttat tttttcgtgc atggggattt atcgatttct
     1321 aatcccttga tatgttaggt atataatcca acactcatgg tcgctctcat aaacatatcc
     1381 cttgagacgc agcagattac aacaaatgaa gccatataaa tgaacagtaa agaaagtttg
     1441 cgcagaagat ttttacaact aatgacagaa aacgttaaat cagagttact tcttctgatg
     1501 gcagataata acgaagcaac aagcagcatt cttgcagacc cttacggtaa gatctcacat
     1561 aaaacgctgg atattattac cacaacatta acaccgctga tgcttcaacg gctgaaacat
     1621 aatatcaacg catgggttaa tgaagaatta agtcctccct gcttatggga ttctcgttac
     1681 gcatgtcagc aaaaaatgcg aattttcaac ttactatcac caaagctcag gtagccataa
     1741 aatcctgccc ttcatggcat acaggatttc aatggaatca caatgaccaa ctcttgcaca
     1801 gctgtatccc tgactccccg acaactcaga ttttcagtat ctgctgctat ctaaagagaa
     1861 agcgcacaaa tgcaagggtc tttcatcacg tcctgttatt gattgcctgt gaccttttct
     1921 tacctcatgg aacgtttttt cagttagaaa tattcatttt acaaccagtt cgtattgttt
     1981 attcatcgac tactctcccc gcgccacctt acgacggtcc tctctgattt tgaaatacag
     2041 gttagtcaga tacgtcagca ggccaaacag cagacttccc agcacaccta ttgccaccca
     2101 ctgggacgga gagactttgt ccagcagctg cagtaaccag tatcccgtcc ccaccgctga
     2161 cgtggtgtat gacacacctg ttgtgatttt ttccatctga tgtatgtctc cgtcaccgcc
     2221 gacagaaaat gaaagtaaag aaaaacaaaa aaaccgccag tgtcacccac tgacggccaa
     2281 ctccgggagc cgtgattatg gcattcaggc tctgctaaaa atgccagata acattccggc
     2341 ctcccctgat tcaggttata aatgacacaa tatcttgaca acacccgtca ctgtctgtca
     2401 gaaaatatac cgccaggcat aagtatcatg tgaaatccaa ctat
//
LOCUS       DAZFWL020000298         2444 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFWL020000298 DAZFWL020000000
VERSION     DAZFWL020000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.302"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(193..507)
                     /locus_tag="ACHKNP_RS25705"
                     /old_locus_tag="pgaptmp_005141"
     CDS             complement(193..507)
                     /locus_tag="ACHKNP_RS25705"
                     /old_locus_tag="pgaptmp_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4707103.1"
                     /translation="MLHDHLAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
     gene            complement(1033..1218)
                     /locus_tag="ACHKNP_RS25710"
                     /old_locus_tag="pgaptmp_005142"
     CDS             complement(1033..1218)
                     /locus_tag="ACHKNP_RS25710"
                     /old_locus_tag="pgaptmp_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4707104.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTVQAPCVKPPSPPAWIMQPV
                     PDWQKPLNGIISSSENG"
     gene            complement(1446..1595)
                     /locus_tag="ACHKNP_RS25715"
                     /old_locus_tag="pgaptmp_005143"
     CDS             complement(1446..1595)
                     /locus_tag="ACHKNP_RS25715"
                     /old_locus_tag="pgaptmp_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707105.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVILVAIFFSTAWRVLTL
                     SGLVG"
     gene            complement(1595..2164)
                     /locus_tag="ACHKNP_RS25720"
                     /old_locus_tag="pgaptmp_005144"
     CDS             complement(1595..2164)
                     /locus_tag="ACHKNP_RS25720"
                     /old_locus_tag="pgaptmp_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGU4707106.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTDKGKITKAD"
BASE COUNT          664 a          627 c          463 g          690 t
ORIGIN      
        1 gcgtgggtta ttgaagcctg cagaagaaga ttaattaatg aaaaatattc tcaatttgta
       61 cccaacaaag acaaacacga ccagagaacc tgttcagaca ggtttactta aacgacttat
      121 atatgacaca aaaagcgacc actaaagtcg ctttttctta tggtaacagg caataacgct
      181 ctcagatatt ttttagcatt tttttgaccg cgcgtttccg gacgtattct gttctcctgt
      241 ccctttatat cgtcggaata cccgccgctc ttcaaatccc attcccaact cagaatgtag
      301 tctgttgacc gcttgtttta tttcggtcag gttcaccggt gaaaccggag tccggcgcgc
      361 cttacgcaaa cactctgctc gtttctgtgc cgccactttt cttttctggt catcacttag
      421 ctgtaccatc acttttgccc atcgttcagc tgctctccgg tacagtcctt ttttctccag
      481 acattctgcc aggtgatcat gtagcataag taacctccgt ttatctacag actgccatcc
      541 tgaatttacc gtccttaata agacaacaat aaaaaactaa ttatataaaa acaataaaaa
      601 taatatacaa aaaaactaaa caacaaagaa aagcaaatca tatctggcat tttaattgaa
      661 agagcaatta ccgaacaaaa gacgctgact atatactcaa aaccaaacaa caattctgcc
      721 aatcaggtat catggcaaca cacggaatta ccatgttttt gccttctctg cccatacaat
      781 acgggcatat acttcatact ctattgtaat atttctatcc atgcgcccca ctccatttac
      841 ctgtaaataa tattcaaaat atttatcaca gaaatcgttt ttggccatga actgagcaca
      901 ctataaagtc cggaactgac tctttgttaa attaccttaa cgttaccagt aacaccttcg
      961 taacaaaaca tcacggtata cactgggtac ggatatattc ctgtgctcct tccagttgct
     1021 tctgcattgc catcagccgt tctctgagga tgaaataatc ccgttcagcg gcttctgcca
     1081 gtcggggacc ggttgcatta tccacgccgg aggtgatggg ggctttacgc aaggagcctg
     1141 gacagttggc gttgatgcgc aggcgcttac gaccagcggc aacatcagca cgcagagttt
     1201 cattttcagc tctcgcatcg gctaattccc tcgagtatct ggcatcaagt gcagcaacat
     1261 cacgctggcg ctgctgcata tcagtaatgg ttgcatttgc cagctccagc tcactgactt
     1321 ttttatcgcg ctgctctttg taggttatgg cgttatcgcg gtaatgattc agccccagac
     1381 taagtgcacc acaggctacc agcaggacaa taatcaccac acacagaaca cggttcatct
     1441 ctctttcacc ccaccagtcc cgataacgtc aggactcgcc aagcggtgga aaagaaaatg
     1501 gcaaccagaa tgactaaaaa tgaaatgccg acgattacac agaggatctt cgccagcgtt
     1561 atgagtttat ccgatatcat tagccaccac tccatcaatc cgcctttgtt attttccctt
     1621 tgtctgtatc agccaggaca aaatcaatca gcagattcgc ttcgtttatc aatgtgcgga
     1681 tttttgatac atgcgcggat ttaacctgtt tccactcatt cagcccggta gcaaacacac
     1741 tggcaatgtt tttatcccgt ttcatgtcag cgcaagcctg attgagttct tccatcacac
     1801 tcattcgacg gggattaacg acaaaaccct tcgtccagta ttcatagaga acatcgtcac
     1861 actcttcctg atactggatt accttgccgc ggatttcggg ttttactttg ttgggattga
     1921 tggtttgtaa ccagcctgca agttttcgaa gcggcaggga caccatattg cgtcgtttcc
     1981 catcctcagc aaccataacg atttccgtta tagttgacgc aaaacgctgt cttaacttag
     2041 ccaactgtga ttgccaggcc agccccatcc ccgcaacgac aggtttcatg ggaacgtatg
     2101 gttcgccgtt atggttaacc acataaagag aatcgccgtg aaacggcacg gtcatcatat
     2161 tcatcggtta tttcctttta gtgatgaacc ctgcgcacag gaataaccag cccaaagagg
     2221 gttaaccaga ccactgccgg ttatccacca gggctcatcc tgaaaggttc tttggtttat
     2281 ttacgcttgt gcgaagcgca gaaatgacaa aggcaccatt acggtgcctc ttcatgaaac
     2341 aatcttgttg actttattca cttacatttt gccagttcgc aggatttcgt gttgtctacc
     2401 cgcgttggcc aacgtcattt ttcagcaaaa tattctgctt acct
//
LOCUS       DAZFWL020000299         2430 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFWL020000299 DAZFWL020000000
VERSION     DAZFWL020000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.303"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            161..376
                     /locus_tag="ACHKNP_RS25725"
                     /old_locus_tag="pgaptmp_005145"
     CDS             161..376
                     /locus_tag="ACHKNP_RS25725"
                     /old_locus_tag="pgaptmp_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4707107.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            376..873
                     /gene="rrrD"
                     /locus_tag="ACHKNP_RS25730"
                     /old_locus_tag="pgaptmp_005146"
     CDS             376..873
                     /gene="rrrD"
                     /locus_tag="ACHKNP_RS25730"
                     /old_locus_tag="pgaptmp_005146"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGU4707108.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            870..1307
                     /locus_tag="ACHKNP_RS25735"
                     /old_locus_tag="pgaptmp_005147"
     CDS             870..1307
                     /locus_tag="ACHKNP_RS25735"
                     /old_locus_tag="pgaptmp_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4707109.1"
                     /translation="MSRVTVIISALVICIIACQSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTSDAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            1457..2074
                     /locus_tag="ACHKNP_RS25740"
                     /old_locus_tag="pgaptmp_005148"
     CDS             1457..2074
                     /locus_tag="ACHKNP_RS25740"
                     /old_locus_tag="pgaptmp_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4707110.1"
                     /translation="MLASLGGLVSCAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(2262..>2430)
                     /locus_tag="ACHKNP_RS25745"
                     /old_locus_tag="pgaptmp_005149"
     CDS             complement(2262..>2430)
                     /locus_tag="ACHKNP_RS25745"
                     /old_locus_tag="pgaptmp_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4707111.1"
                     /translation="TTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEACRRRLTSEK
                     RAYTSIQSDDG"
BASE COUNT          638 a          548 c          636 g          608 t
ORIGIN      
        1 tcatttataa cctgaatcag gggaggccgg aatgttatct ggcattttta gcagagcctg
       61 aatgccataa tcacggctcc cggcgttggc cgtcagtggg tgacactggc ggcttttttg
      121 tttttcttta ctttcatttt ctgtcggcgg tgacggagac atacatcaga tggaaaaaat
      181 cacaacaggt gtgtcataca ccacgtcagc ggtggggacg ggatactggt tactgcagct
      241 gctggacaaa gtctctccgt cccagtgggt ggcaataggt gtgctgggaa gtctgctgtt
      301 tggcctgctg acgtatctga caaatcttta tttcaagatt aaagaagata agcgtaaggc
      361 tgcgagaggt gaataatgtc gccgtcatta cgcaaggctg ttgcagctgc tattggtggt
      421 ggggctgttg ccatagcgtc tgtgctcatc actggtccag gtggtaacga tggtctggaa
      481 ggtgtcagct acataccata caaagatatc gttggcgtat ggactgtatg tcacggacac
      541 accggaaaag acatcatgcc cggtaaaacg tataccgaag cagaatgcaa agccctcctg
      601 aataaagacc ttgccacggt cgccagacaa attaacccgt acatcaacgt cgatataccg
      661 gaaacaacgc gcggcgctct ttactcgttc gtttacaacg tgggcgctgg caatttcaga
      721 acatcgacgc ttcttcgcaa aataaaccag ggcgatatca aaggcgcatg tgatcagcta
      781 cggcgctgga catacgctgg cggtaagcaa tggaaagggc tgatgactcg ccgcgagatt
      841 gagcgtgaag tctgtttgtg ggggcaacaa tgagcagggt aaccgttatt atctccgctc
      901 tggttatctg cattatcgcc tgccagtcat gggctgttaa tcattaccgt gataacgcca
      961 tcgcctacaa agagcagcgc gataaagcca catccatcat cgctgatatg cagaagcggc
     1021 aacgtgatgt agcagaactt gacgccagat acacaaagga gcttgctgat gctaatgcga
     1081 ctatcgaaag tctccgtgct gatgtttctg ctgggcgtaa gcgcctgcaa gtctccgcca
     1141 cctgtgcaaa gtcaacgacc ggagccagca gcatgggcga tggagaaagc ccaggactta
     1201 catcagatgc tgaactcaat tattaccgtc tccgaggtgg aatcgacaag ataaccgcgc
     1261 aggttaacta cctgcaggaa tacatcagga cgcagtgctt aaaataattt taatttcact
     1321 gaaatttaac aagtgacttt caggaaaatg cctcgcagat gcggggcatt tttgtaccgg
     1381 tatttcaccg cgcaccgcag cgcacaataa acaccgaacc tgaccctttg gaatgggcct
     1441 ttgaggatac cagttagtgc tggcgagcct cggtgggctg gtttcctgtg cggcaaaggt
     1501 tcatttcaaa gaagcaggca acgccatgaa tgaattaatt gcgaatcatg acttcgactt
     1561 tcgccagtta gttaccgcag cagaaggtca accggtaact gacaccttcc agattgccag
     1621 ggcatttggt aaacgccatc agcatgtgat tagggctatt aaatgtttga gatgttctga
     1681 ggaattctcg acaacccatt tttgggccgt cgagaaaatc aatgacttag gtatttttga
     1741 caagaaacag atttactacc gcatggactt tagtggcttc gttatgctgg ttatgggatt
     1801 taacggggca aaagccgatg ctgttaaaga agcctatatc aatgcgttta actggatgtc
     1861 agcagaactc cgtaagtaca gcgaaagtta tgaagcagaa cgtaacgccg taatgctgga
     1921 gtacatgaaa gagaaggatg tcgccagcat gtcaggccgt ctgctcaatc gctgggggag
     1981 aacgaaaaaa cctcaattgc ttgcaaagct ggaacgtctg gagagacagg gacagttttt
     2041 attaccggga ttcgataaag gtattcaagc ctgacacatt atgcgctgta tcgtcgccgt
     2101 attcccgcat taaccatgac cgtagcccga cggggaattc cttctgcgtg agtgtgcggg
     2161 aataatcaaa aacgatgcac accgggtttt actgtgctga cagacgcagg gttaccctca
     2221 tagtcgcttt tccggtgcga tggtggaaga aaccgggatg ttcatccatc atcactttgg
     2281 attgatgtat atgctctctt ttctgacgtt agtctccgac ggcaggcttc aatgacccag
     2341 gctgagaaat tcccagaccc tttttgctca agagcgatgt taatttgttc aatcatttgg
     2401 ttaggaaagc ggatgttgcg ggttgttgtt
//
LOCUS       DAZFWL020000300         2422 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFWL020000300 DAZFWL020000000
VERSION     DAZFWL020000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.304"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1831)
                     /gene="nleL"
                     /locus_tag="ACHKNP_RS25750"
                     /old_locus_tag="pgaptmp_005150"
     CDS             complement(<1..1831)
                     /gene="nleL"
                     /locus_tag="ACHKNP_RS25750"
                     /old_locus_tag="pgaptmp_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HGU4707112.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSN
                     FSNSNLKNASFKGSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTI
                     NLGGCYIDLSDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIKKISL
                     DELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIMMYMEN
                     GSLLQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDGMQERFRELYEVYLKNEKVYPVT
                     QQSDFGLCDGSGKPDWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTSTNWM
                     SFFLYKDGEVQNTFGYSLSNLFSESFPIFSIPYHKAFSQNFVSG"
     gene            complement(1851..1940)
                     /locus_tag="ACHKNP_RS25755"
                     /old_locus_tag="pgaptmp_005151"
     CDS             complement(1851..1940)
                     /locus_tag="ACHKNP_RS25755"
                     /old_locus_tag="pgaptmp_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707113.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            complement(1953..2045)
                     /locus_tag="ACHKNP_RS25760"
                     /old_locus_tag="pgaptmp_005152"
                     /pseudo
     CDS             complement(1953..2045)
                     /locus_tag="ACHKNP_RS25760"
                     /old_locus_tag="pgaptmp_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(2047..2316)
                     /locus_tag="ACHKNP_RS25765"
                     /old_locus_tag="pgaptmp_005153"
     CDS             complement(2047..2316)
                     /locus_tag="ACHKNP_RS25765"
                     /old_locus_tag="pgaptmp_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4707114.1"
                     /translation="MNILKKLMQRLCGCGKHDDRENGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(2318..>2422)
                     /locus_tag="ACHKNP_RS25770"
                     /old_locus_tag="pgaptmp_005154"
     CDS             complement(2318..>2422)
                     /locus_tag="ACHKNP_RS25770"
                     /old_locus_tag="pgaptmp_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4707115.1"
                     /translation="QGFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          768 a          546 c          380 g          728 t
ORIGIN      
        1 taccagaaac gaaattctgg gaaaaagctt tatgataagg aatactgaaa attggaaatg
       61 attcagaaaa aagattgctc aatgaatacc caaatgtatt ttgaacttct ccatccttat
      121 ataaaaaaaa ggacatccag ttagttgatg tattaggaga taacatatca accatatgac
      181 tcaaagaaca catcattgcc ataccatcat cctgtgatga taacaaaacc cagttataag
      241 ccaaatcgga atcatcatcc cagtcaggct tcccagagcc atcgcacaat ccaaaatcac
      301 tctgctgtgt gacaggataa actttttcat tttttaaata tacttcatag agttccctaa
      361 atcgttcctg cattccatca cgccgtgaac cattcattac aatttgtata aaggaactat
      421 tataagatac catcgcacct ggatgtcgct caaaatacat ccctgcctgc aataaactac
      481 cattctccat atacatcatt attgaattat taaaccaaga cattatagta tcaaaaaagt
      541 catcacatat aggctcgagc cattgctgta tatttgcgtt gttcaaataa ggatgaatac
      601 taaatgaatc agccaaagac atcttaacat agggccggaa cgccgctaat tcgtcaagag
      661 atattttttt gataagttct tcagcggccc ttactttatc atggttatac ttatcatcag
      721 atgtattcat agtcaataaa attgatttat ttgattttgt atagttatcc aacacagata
      781 atatattttc ctgtatgtca gatagatcta tgtaacaccc tcctagatta atggtattac
      841 ttttttcact aaagagctcc tttacatcac ctaagatagc accacttaaa accattccag
      901 gaattataat agctcctgtt aaatcagcct cgttcaaaat tggaggatat tgtatatatg
      961 aagatccttt aaatgatgca tttttaagat tggaattgct gaaattagag ttttcaaaca
     1021 aagccctaat aatacaatta gataaattag cataacaaaa attacattca ttcaaaatcg
     1081 agtttttaaa agaacagttt tccaatacag cctgagacaa atcactcatc ctgagatctg
     1141 tatcatcaag cactgctcca gaaaaattta ctgatgataa attcagccct acaagactta
     1201 gacctgacaa gtcacatcca gagtaattca attcttcagc agactcttca cccgttcggt
     1261 tagccgacaa ccaaattaag tcagcagtaa gctcagcttt gctcaggcag gcacggcctt
     1321 gactgacatc atatgcaagg aattgacatt gcgattcatt taatgaatta cagccattta
     1381 aatatacgga atttctgagt gcctcaggaa aggaaccttc tatatgtttc aaagaactac
     1441 aatggcataa agataaatta atgacatttt caggtatgct gcaatttata acctctaatg
     1501 atgagcatcc aaccatactt aatgaagata agttgggggg taagcagttt attgatttaa
     1561 gctctgtaca tccattcaaa accagttctt tcagagaacc tggaagcaag tctggtaatg
     1621 ttgttattgg ctcactgatt gataaagtct ctccattagt acttataaca tcaagtattt
     1681 ttgatgcaac ctcatgacga ttttcgctaa attctccctc agcgcaccac ttttcgaggg
     1741 caacttcaac atcctcgtta gatggtgaat ctacaggact ttcaaaagat attactccag
     1801 aatttacaga gatatttgta gtgggcagca tttatctcac tcctttttac ttagtccact
     1861 aaagtaaaat tagaaaacac aactgccggg acaactttct gtccctgtac ccacaaataa
     1921 gatgtcagat ttatcggcat taatttaact ccctattgaa ttatctcctc ttttttatct
     1981 aacaaatatt ccccggacat gacaacaaaa accggagccg gactccggtt ttgtgaagct
     2041 gtcgggttac ttcatcccgc caatattttc ccacgtcccg tcagcacgca ggatttgcag
     2101 cggtcttacc acgcactgta tctgcttttt atccgcatcc agtatcacca cctgcgtgat
     2161 taccctgtcc tgctccggga taatgccatt ctcatctgac tccaggatgt ctgccggccc
     2221 cagtcgcagc tgtgctgtaa gtaactcccc gttttcacgg tcatcatgct ttccgcaacc
     2281 gcacagacgc tgcataagtt tttttagtat attcatgtca ttctcctgtt ctgcctgtat
     2341 cactgcccac ttcatccagt cccttaacat cctgccacgg cccgtcgcca aatctgacct
     2401 gcaaatgctg aaaaaaaccc tg
//
LOCUS       DAZFWL020000301         2422 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFWL020000301 DAZFWL020000000
VERSION     DAZFWL020000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.305"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..494)
                     /locus_tag="ACHKNP_RS25775"
                     /old_locus_tag="pgaptmp_005155"
     CDS             complement(<1..494)
                     /locus_tag="ACHKNP_RS25775"
                     /old_locus_tag="pgaptmp_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU4707116.1"
                     /translation="MRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIALERRSKEREK
                     AEKAAEKKLRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISCG
                     TLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIGQEA
                     VEEI"
     gene            complement(469..1035)
                     /locus_tag="ACHKNP_RS25780"
                     /old_locus_tag="pgaptmp_005156"
     CDS             complement(469..1035)
                     /locus_tag="ACHKNP_RS25780"
                     /old_locus_tag="pgaptmp_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4707117.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGTFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
     gene            1559..>2422
                     /locus_tag="ACHKNP_RS25785"
                     /old_locus_tag="pgaptmp_005157"
     CDS             1559..>2422
                     /locus_tag="ACHKNP_RS25785"
                     /old_locus_tag="pgaptmp_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707118.1"
                     /translation="METINSGGNLIPNSTSNKGYVCIDMQCSSTSEPTASTSSNRSIK
                     LAASTNVYPITRNDSELTLNDFLDNSSSTSSLDYINELGSQLTLNDFLDNIKTNEVDR
                     TCTDVVINIPQEIQTNTQENDLLLSDKNNSICIEIDERITKILTCKQKYQLDSIIHEI
                     IPKENESAETVLHLMRVLNDQYHQVYNQSGCFYKAYMAIHNKIEQILPYAFRAGGGIS
                     IHLLIQALFFNGDYNKSPSQSQQPSLYTSPSPTINTEAFLSNVLSLDITQVRILGDLL
                     SATLFHAPTIFY"
BASE COUNT          747 a          517 c          409 g          749 t
ORIGIN      
        1 tcgatttcct ctactgcttc ctgcccgatg cggctaatca gttcgacgcg atacggaacg
       61 agatttccgc ttttgtgctg gttgcacacc acgcattgct tgtgaatatt gcgttcatca
      121 aatcggagtt gaggtgccgc agcagttgtc cggtaatgtc cggcatccca ctgagcagac
      181 gtgagcgttc cgcacgagat acatggtaag tcgcggtctc tttctctgat gaaggcgttt
      241 acggcttgtt gggcttgttt aatccagtaa ctgcggggct ttaaggcgag ttttcgaatc
      301 ttcagtttat ctttctgttt ctgctcctct cgtcgtagtt tcttctctgc tgctttttcc
      361 gctttttcgc gttctttact tcgtcgttcg agtgctatct tggttccaca aatctcatta
      421 caccaatatt gattttgata ttttggtata aaccattcat tgcaacattt acatttcctt
      481 cgatagattc gcataagtgc tcctttcgtt gccggaaaaa tcaccgtaat acttatctcg
      541 ggcttcttca gcaactagta ccgctaactc cagatcatca aagcatccga agtgtttact
      601 cttgccatgg aatcctagcc taacattcca ttttttctgt cgtttgtgcc aagtaacccc
      661 tctgcaacct gatttgctat tctttcggat ccttatattt cttgaatttt ctattggcag
      721 gcattctctt aaattttctg gcctattgtc ggtcctaatt ccattaacgt ggtcaatttg
      781 accagcaggc caacgattat gagttatgta aaaaactaag acgtgagttt tatatctacg
      841 cccatctatc atgatcattg aataaccgtt ggaatcaaaa gttccagcaa cactatttaa
      901 tgctatcctt ccctgagtgg gaactttcca tctaaatacc ccggtagatt tatcgaaact
      961 tagtaactca aatatccttt taacagttaa atcttctctt ttacggttac atcgtcttcg
     1021 cgctggttta gccatcgcct tcttcctccg taatggtttt ctgaatttgg ccacctgaac
     1081 agagctcacc aaagctatgg atgtcggtat ttccacaata ccaagatggc gaaaataact
     1141 gcatgataag cctcagggaa aagggaagac actacccccg ataattcaga aacaaatcga
     1201 aatacatgaa ctaaagaaaa aaccacaaca aacagaaata gaaatagaaa tagaaataga
     1261 aatagaaata ttaaaacaga ccaccacatt cctgatgtca tactcactaa acaatttttc
     1321 gacaacatgg tagctcatag aacgttatcg tgtagacacc ctctgctacg tattcggtgt
     1381 tcagtgcaat atctacaaat actggaaaaa tctaaaatcg aggcaagttt ttggtgataa
     1441 ctatagttag actatattga cgacctgatg tgctgtatgt aataactaac aaaaaatatt
     1501 ttccatggga ttttttattt taatgaaatg caaaatattt ttatcaatag ttagtattat
     1561 ggaaaccatt aattcaggag gaaacttgat tccaaattca acttcaaata aaggttatgt
     1621 atgcattgac atgcagtgtt cgtcaacctc tgaaccaaca gcttctacct ccagcaaccg
     1681 gagtattaaa ttagccgctt ccacaaatgt atatccgata acaagaaacg actccgagct
     1741 cactctgaac gattttcttg ataatagctc ttctacgtca tcattggact acattaatga
     1801 attgggttct caactgacgt taaatgattt tcttgacaac ataaagacaa atgaggtgga
     1861 tagaacatgt acggatgtgg taattaatat cccacaagag atacaaacaa atacacagga
     1921 aaatgatttg ttattatccg ataaaaataa ttcaatatgc attgaaatcg atgaaagaat
     1981 tacaaaaatc ctaacatgca agcaaaaata tcaactggac agcatcattc atgaaattat
     2041 accaaaagag aatgagagtg cagaaactgt tctccatctt atgagagttc tgaacgatca
     2101 atatcatcag gtatataatc agtcaggatg tttttataaa gcctatatgg ccatacacaa
     2161 taaaatcgaa cagatacttc catatgcgtt cagagccgga ggcggaatca gcattcactt
     2221 gctcatacag gcattatttt ttaatggcga ctataacaaa tcaccttcac agtctcaaca
     2281 accatcttta tatacatcac cttctccaac aataaataca gaagcattct taagtaatgt
     2341 attatcacta gatataaccc aggtacgcat acttggtgat ttactatcag caactttatt
     2401 tcatgcacca acaatattct at
//
LOCUS       DAZFWL020000302         2422 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFWL020000302 DAZFWL020000000
VERSION     DAZFWL020000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.306"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            228..476
                     /locus_tag="ACHKNP_RS25790"
                     /old_locus_tag="pgaptmp_005158"
     CDS             228..476
                     /locus_tag="ACHKNP_RS25790"
                     /old_locus_tag="pgaptmp_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4707119.1"
                     /translation="MRVEICIAKEKISKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(473..559)
                     /locus_tag="ACHKNP_RS25795"
                     /old_locus_tag="pgaptmp_005159"
                     /pseudo
     CDS             complement(473..559)
                     /locus_tag="ACHKNP_RS25795"
                     /old_locus_tag="pgaptmp_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            644..727
                     /locus_tag="ACHKNP_RS25800"
                     /old_locus_tag="pgaptmp_005160"
                     /pseudo
     CDS             644..727
                     /locus_tag="ACHKNP_RS25800"
                     /old_locus_tag="pgaptmp_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            complement(848..1861)
                     /gene="nleC"
                     /locus_tag="ACHKNP_RS25805"
                     /old_locus_tag="pgaptmp_005161"
     CDS             complement(848..1861)
                     /gene="nleC"
                     /locus_tag="ACHKNP_RS25805"
                     /old_locus_tag="pgaptmp_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HGU4707120.1"
                     /translation="MYKTILSINMKIPVLQPGFNFFAPAGYSAAVAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVISAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEEVHIGCIKYRNEYELNGESAIKIDDIQSLTCNELYGYDVGQEPILPICEA
                     GENENEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYADPEREAHLRLRNLNALRQAAMRHEKNERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGVAIDENLQDVNQIQLYHGAPYIFTFGDVD
                     RHN"
     gene            complement(2264..>2422)
                     /locus_tag="ACHKNP_RS25810"
                     /old_locus_tag="pgaptmp_005162"
     CDS             complement(2264..>2422)
                     /locus_tag="ACHKNP_RS25810"
                     /old_locus_tag="pgaptmp_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4707121.1"
                     /translation="LESDENGIIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGT
                     WENIGGMK"
BASE COUNT          684 a          579 c          481 g          678 t
ORIGIN      
        1 tgacgcaaca tttttaaaaa tcgacgaaga tcgtccagga gcgccgtttt tcaagggttg
       61 gatagatgat tcgtttacca tgtcgacgaa cttcgacgaa tttcgccgat aaaaagtgtc
      121 ccatacatgc cccattgatg ccccataaac aatatcagga attgacactg gttatccata
      181 cagataaaaa taacacctgt atataaccac agttacagga gggtgttatg cgtgttgaga
      241 tctgtattgc taaagaaaaa atcagcaaaa tgccaaacgg tgctgtggat gcgttaaagg
      301 aagaattaac ccgacgcatc agtaaacgtt atgacgatgt agaggtgatc gtaaaagcca
      361 ccagcaacga tgggctttct gttacacgca ccgcagataa agattctgca aaaacatttg
      421 ttcaggagac tctgaaagat acctgggagt ctgctgacga gtggtttgtt cactaataaa
      481 cacgtaaaat cggtaacggc tggaaatcat tcaatactcg cactatcgaa agttcaccag
      541 ccaaccgcga cacgttctta catacgaagt gccgcgcttt ccttaataat tttttagcag
      601 tactgtgtaa atgatgagcg acctaatcca tgcatgacgc gtagtgccta ttgtgcatct
      661 ttctgcgtct ctttttactg gacccaagcc aatgatgctg gcgataccat tcccgccagg
      721 cccacttaca acagtaaaat tcggggctct gcctttacat acaatacaat gttgcacgat
      781 tgcccatcat caactccgtt aatatctatg agtagtaacc ctatgtaact gtaaagacga
      841 atcagcgcta attgtgtctg tccacatccc caaatgtaaa gatataagga gcaccatgat
      901 acagttggat ctgatttaca tcctgtaaat tctcgtcgat agccacccct ggaaaatcat
      961 gttgctggat aaatccgtat cctatgttag acacagcgga atactctgtg aaatcaggac
     1021 tcgcctcata tcggtcactg atcgtaccca gtctttcgaa gaaagccctc tcattctttt
     1081 catgcctcat ggcagcctgt cgaagggcat tcaggttacg tagtctaaga tgagcttctc
     1141 gttctggatc tgcatatcct ttgaagtcgg gaacactcca tcccagctct tgagcgacac
     1201 gacgtgcgag aatctcggtg ggtcctagct ctatattact atctccagat ggatcgctgg
     1261 ccccagtaac atgatgaata atctcgtgaa tcagtccttc ctgccacgat ggcatctcat
     1321 aagaatcagt atctggcgca acactaaaac tgacataagg ctcttcgttc tcgttttctc
     1381 ctgcctcgca aatggggaga attggttctt gcccgacatc gtatccatat aattcgttac
     1441 aggttagtga ttgaatatca tcaattttga tagcagattc tccgttaagc tcgtattcgt
     1501 ttctgtattt aatgcaacca atgtgtacct cctcattatg aatgccatag cttacggcat
     1561 cacgaaatgt ttgtgatcgg ctcaacgcat caagtacagt gttgccaatc atatcgaccg
     1621 tatgctgatc gatgagcctg ctacggctgt catggacggc gctaattaca ctttcgtata
     1681 cgttgcttaa atctgctgcg gaaagcggta ttcgcttgcc tatatccaaa acgtaatccg
     1741 cataggcatt ttcagcacga ttaggagcaa cggcagcaga gtatccagca ggggcaaaaa
     1801 agttgaagcc aggttggaga acgggaattt tcatattgat acttaatatg gttttatata
     1861 tttcagtgcc accaattcac tagcttacaa ccagaaacta aaaaaacgtc agggcgttga
     1921 taataatgaa taccaacttt ctgcccattt atagtaatat ctttcgttat caggttttgt
     1981 ctggcagctt cacgtaatat ttcaatactc tgcaaagcaa catttgatga aatgctggat
     2041 gttatcaatg gcatatgtca tttaccccaa caagactaaa ttttagaaaa aacaactgcc
     2101 gggacaactt tcgccccctg taaccccaaa taagatgtca gatttatcgg cattaattta
     2161 tctccctgtt gaattagctc ctctatcatc tacttaacaa atatcccccg gacattgcaa
     2221 cacaaaaacc ggagccggac tccggttttg tgaagctgtc gggttacttc atcccgccaa
     2281 tattttccca cgtcccgtca gcacgcagga tttgcagcgg tcttaccacg cactgtatct
     2341 gctttttatc cgcatccagt atcaccacct gcgtgattac cctgtcctgc tccgggataa
     2401 tgccattctc atctgactcc ag
//
LOCUS       DAZFWL020000303         2421 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFWL020000303 DAZFWL020000000
VERSION     DAZFWL020000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.307"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(41..307)
                     /gene="ariR"
                     /locus_tag="ACHKNP_RS25815"
                     /old_locus_tag="pgaptmp_005163"
                     /gene_synonym="ymgB"
     CDS             complement(41..307)
                     /gene="ariR"
                     /locus_tag="ACHKNP_RS25815"
                     /old_locus_tag="pgaptmp_005163"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HGU4707122.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(336..608)
                     /locus_tag="ACHKNP_RS25820"
                     /old_locus_tag="pgaptmp_005164"
     CDS             complement(336..608)
                     /locus_tag="ACHKNP_RS25820"
                     /old_locus_tag="pgaptmp_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HGU4707123.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(651..887)
                     /gene="ycgZ"
                     /locus_tag="ACHKNP_RS25825"
                     /old_locus_tag="pgaptmp_005165"
     CDS             complement(651..887)
                     /gene="ycgZ"
                     /locus_tag="ACHKNP_RS25825"
                     /old_locus_tag="pgaptmp_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HGU4707124.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            1201..2412
                     /gene="bluF"
                     /locus_tag="ACHKNP_RS25830"
                     /old_locus_tag="pgaptmp_005166"
     CDS             1201..2412
                     /gene="bluF"
                     /locus_tag="ACHKNP_RS25830"
                     /old_locus_tag="pgaptmp_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HGU4707125.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT          706 a          495 c          461 g          759 t
ORIGIN      
        1 tgaatgagtt ttctataaac ttatacttaa taattagaag ttacatatca tcagctgtgt
       61 atcgcaacac gatttccagt gtttttctaa tcacatcagc tttgagaata tcacttgttg
      121 tttccaggga gtgtatcaga cttaagataa tatttttatt attaacatta tcgcctgaaa
      181 gcattaaatt ggtgacagcc tgccctaaca ctgctgactc ttcttctaac aaattacccg
      241 aactgcgaaa gtaacttgct aacgctttat cagttattgc attatgaatt gtagtatctt
      301 caagcatggt aacctctcat cttacttatg aaattttaat gtattctgtt tattttctta
      361 ccattgaaca tacttttatt gtcagctgtc gctgttcttc ggtttggccc tcctgaatca
      421 ctattgctaa aatcacgaat tatgctgtca attaaatcgc gtcggaaagc atcttcctca
      481 gccgatttca ttacgagtaa ttgcttaata agagtctgca atgaaaaatt tatcttcatg
      541 cgcagtatct ggatcaccgc ctggccagta atttcatatt ttatacgttc attatcagat
      601 gtcttcataa cgacttcata gctaacctgt taaataatca ggctgttgca ttattcaaaa
      661 agcaacccaa ttagtgcatt ataatgtttc tgttcctctt ccccagtcgc gtgttccagt
      721 cggcaaagaa gtttggcaca aagcgacttt cgactcagat tacgtccatc tttcaaaatc
      781 tcagtcacaa tttccccgag agtttcctgc tgagtaggca agttagcttt tgcaaagtaa
      841 cgagtgattg ctcccgcaga atctaaagtc actgaatttt gatgcatgct acgcctctgt
      901 taaaaatgtt aatcaatgtt aagtgaggta acaatagcaa acttgtacga aatatgtgta
      961 cagaatgata aattaatttt ttgcaattag tgctaatgca tataaaacaa tgtgttatga
     1021 taaaacatat tgcatcattt aactgacaat ctatacaaga tttttcgcca actagtgttc
     1081 aactttttcc attttttatc aatgtaaaga aacggttatg ttttggtatt aaaagaaaga
     1141 cacaagaaga tatgtctgtt accgtcttac tctcgcctca cccattaccc tggattgtta
     1201 atgcttacca cccttattta tcgtagccat atacgtgacg acgaacctgt caaaaaaatc
     1261 gaagaaatgg tttcgatagc aaatcgcagg aacatgcagt ctgacgtaac agggatctta
     1321 ctgtttaatg gttctcattt tttccagctt ctggaaggtc cggaagaaca ggttaaaatg
     1381 atatatcggg ctatatgcca ggatccacgg cactataata ttgttgagct gctgtgcgat
     1441 tacgcgcctg ctcgccgttt tggcaaagcg ggaatggaat tatttgattt gcgcctgcac
     1501 gagcgagatg acgttttaca ggccgtattc gacaaaggca catcaaaatt tcagctaact
     1561 tatgatgaca gagcgctaca attttttcgt acttttgtcc ttgcaaccga acaatcaacc
     1621 tatttcgaga tccctgccga agactcctgg ctttttatcg ctgacggatc tgataaagaa
     1681 cttgattcct gtgccctttc accaactata aacgaccact ttgcctttca tcctattgtc
     1741 gatcccttat cgcggcggat aatcgctttt gaagccattg tgcaaaaaaa tgaagatagc
     1801 ccatcagcca tagcggttgg gcagcgtaaa gacggggaaa tctacacagc ggatctcaaa
     1861 agtaaggcgc ttgcattcac gatggcacac gcacttgagc tcggtgataa aatgatttca
     1921 atcaatctat tacctatgac cctggttaac gaacctgacg cagtctcttt tttacttaat
     1981 gaaataaagg ccaatgctct ggtgcctgaa caaatcatcg ttgaatttac tgaaagtgaa
     2041 gtcatatctc ggtttgatga gtttgccgaa gcgattaaat cgctaaaggc tgctggtatc
     2101 agtgtagcaa ttgatcattt tggcgcaggt tttgctggtt tgttactcct gtcacgcttc
     2161 cagcctgaca gaattaaaat cagtcaggaa ttgattacca atgttcataa aagtgggcca
     2221 cggcaggcaa ttattcaggc gatcataaaa tgctgtacat cacttgaaat tcaagtcagt
     2281 gctatgggcg tggcaacacc agaagagtgg atgtggcttg aatccgcagg aattgagatg
     2341 tttcagggag atctgtttgc taaagctaaa ttgaatggta tcccttccat agcgtggcca
     2401 gagaaaaaat aattttcagc a
//
LOCUS       DAZFWL020000304         2404 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFWL020000304 DAZFWL020000000
VERSION     DAZFWL020000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.308"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            188..277
                     /locus_tag="ACHKNP_RS25835"
                     /old_locus_tag="pgaptmp_005167"
                     /pseudo
     CDS             188..277
                     /locus_tag="ACHKNP_RS25835"
                     /old_locus_tag="pgaptmp_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
     gene            282..690
                     /locus_tag="ACHKNP_RS25840"
                     /old_locus_tag="pgaptmp_005168"
                     /pseudo
     CDS             282..690
                     /locus_tag="ACHKNP_RS25840"
                     /old_locus_tag="pgaptmp_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1337..2314
                     /locus_tag="ACHKNP_RS25845"
                     /old_locus_tag="pgaptmp_005169"
     CDS             1337..2314
                     /locus_tag="ACHKNP_RS25845"
                     /old_locus_tag="pgaptmp_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HGU4707126.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
BASE COUNT          646 a          547 c          585 g          626 t
ORIGIN      
        1 ggctgagagc cgcatcggag aagttgcgtc cattttcagg ggaatggggt aaaaaaccat
       61 cagtgatatg cagtatcaat cgataagatc catttaattg acggcggtgc actcatggca
      121 cgcaggcagt gttgaataaa catccgtttt tgggtgtttt tttaatcttt ttgggattta
      181 aattcctatc gatgaacaat taattatcgc tgttgtagct gtgggtaaac gtgagcgtag
      241 tgacgtttat aatcttgcca gtgaaagaat gagataatta acggcggatt atcggttcag
      301 ccctctcatt aaccgctgat gctgagttgg tgtgtatggc tttgcgtaat gcactggaaa
      361 cacgaccgcg tgatggtcgc ctgctgtttc attcagatca aggtgttcag tataaaagca
      421 ataaatacag aaagttactc tggcgttatg gggtaatgca aagtatgagt cgcagaggga
      481 attgtctgga taattcgccg atggaaaaag tctttcgcag ccttaaaagt gaatggcttc
      541 cgaaaggtgg ttatggtgat tgccatgcag tacgcgaata aatcagtgga taaatgctta
      601 ttacaacgtt tatcgacctc atacaaacaa tggtggttta tcgccttgcc gcatgaagaa
      661 aaatggaaac aggttattcc ggtgtcctga ttttatgatc cactacacat tcctgacgaa
      721 cgccactgca taaatcacat tgcacatcct cagagagtac gcagtatagg aggggtatat
      781 atacattatc ggtatatcag gcgacttctg tctgtatgtt gtcggtgctc gccgcagacc
      841 ggtgaccggg cgaagcccgc gaaccggcga ggaagcgcaa tgtatctgta gttacagtga
      901 gcagatacgc cctgaagctt cctcataaaa cagttgtgta aactgttttc ccggaatgat
      961 ttatccacat atccggcggc ccgatccagt aaacagatcc ataaatgatc aagaaatgat
     1021 ccattaaaga tccccatatc gctgcaaacc ttgtcactca tgctccggga ccacgatcac
     1081 ataagctgtg gcatgtttct gataaactgt agcaggatca tgataagctg tattcagtaa
     1141 tgtatatact gtagtaagtt aatgacataa attatggtca gtacgccagg ctcagctgtt
     1201 aaatacagac tgcaggtttt tcttcagtca gttgacgggg ttctgacact caattttctg
     1261 tttattcttt tactgtccac aggcaggagg ctttctggaa aacgaaaatt cagacatcaa
     1321 aaaactgttc ggcgaggtgg ataagtcgtc cggtgagctg gtgacactga caccaaacaa
     1381 taacaacacc gtacaacctg tggcgctgat gcgtctgggc gtttttgtac cgacccttaa
     1441 atcactgaag aacagtaaaa aaaatacact gtcacgtact gatgccacgg aagagctgac
     1501 acgtctttcc ctggcccgtg ctgagggatt tgataaggtt gagatcaccg gcccccgcct
     1561 ggatatggat aacgatttca agacctgggt gggaatcatt cattcctttg cccgccataa
     1621 cgtaattggt gacaaagttg aactgccttt tgtcgagttt gcaaaactgt gtggtatacc
     1681 ttcaagccag tcatcccgca ggctgcgtga gcgcatcagc ccttccctga aacgcattgc
     1741 cggtaccgtg atctcctttt cccgcaccga tgagaagcac acccgggaat atatcaccca
     1801 tctggtacag tcagcctact acgatactga acgggatatt gttcagttac aggctgatcc
     1861 ccgccttttt gaactgtacc agtttgaccg gaaagtgctg ctgcagctca aggccatcaa
     1921 tgccctgaag cgacgggagt ccgcccaggc actttacacc tttatagaga gcctgccccg
     1981 ggatccggca ccgatatcgc tggcgcggct gcgtgcccgc ctcaatctga agtctcctgt
     2041 attttcccag aaccagacgg tcagacgggc aatggagcag ttgcgcgaga ttggatatct
     2101 tgattacacg gagatccagc gggggcggac aaaattcttc tgtattcact accggcgtcc
     2161 ccggttaaaa gcgccgaatg atgagagtaa ggaaaatccg ttgccacctt cacctgcgga
     2221 aaaagtcagt ccggagatgg cggagaaact tgccctgctt gaaaaactgg gcatcacgct
     2281 ggatgacctg gaaaaactct tcaaatcccg ctgaacataa actgtagtca gtgaagagtg
     2341 ctcctttact gactacagct tatattatca ggtgcagtga gtggtctgct cactgcagtt
     2401 tata
//
LOCUS       DAZFWL020000305         2381 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFWL020000305 DAZFWL020000000
VERSION     DAZFWL020000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.309"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            30..335
                     /locus_tag="ACHKNP_RS25850"
                     /old_locus_tag="pgaptmp_005170"
     CDS             30..335
                     /locus_tag="ACHKNP_RS25850"
                     /old_locus_tag="pgaptmp_005170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707127.1"
                     /translation="MITTETAANTAAAIAGATGNASKDTGNDNGWDIGGGCEGSHQLG
                     MDASKYSLQYSEKSIGYGMYQGGQMSSIPGTFGGFVGSFVSEGASSATQKEVDGDKK"
     gene            589..969
                     /locus_tag="ACHKNP_RS25855"
                     /old_locus_tag="pgaptmp_005171"
     CDS             589..969
                     /locus_tag="ACHKNP_RS25855"
                     /old_locus_tag="pgaptmp_005171"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707128.1"
                     /translation="MVSYGQAVSSWNQYADANNLTPEQKQAGLDKLAKGELPEGANIS
                     KVIVDGYKDGVLIAGAWYLGPAASTGKVVSGAVISAIANGTYQWYDLSKPGNENKSWD
                     YWEVPRQALLERWLQVEIYGRTQG"
     gene            1450..2346
                     /locus_tag="ACHKNP_RS25860"
                     /old_locus_tag="pgaptmp_005172"
                     /pseudo
     CDS             1450..2346
                     /locus_tag="ACHKNP_RS25860"
                     /old_locus_tag="pgaptmp_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
BASE COUNT          677 a          420 c          670 g          614 t
ORIGIN      
        1 tttaggtagt tgagaacaat atactgaatg tgataaccac tgaaacagca gctaatacgg
       61 cagcagcaat agctggtgct acaggtaatg ccagtaagga taccgggaat gataatggct
      121 gggatattgg tggtggttgt gaaggctcac atcaactggg aatggacgcc agtaaatatt
      181 cactgcaata ttcggaaaag tctatcggat acggtatgta tcaaggcggc caaatgagtt
      241 ctataccagg aacatttggt ggatttgttg gttcattcgt atcagaaggt gcaagcagcg
      301 caacgcaaaa agaagttgat ggagacaaaa aatgaaaaaa catctactac ttcttattaa
      361 gttgttactt atatctatag ctacttgctc tattggaata gtggttagta tctggtttat
      421 agctcgggaa ccaatacctt ctgaggtgta tgtaaaatac ggaataattg gcgcagtagt
      481 tggcttatgg ggaggtgctg ggatttggtt acaactctat ctacaaatgc gaagatacta
      541 tcgtaaatag ttgttgttga gaataatgcg ctcagtctgc catcaggaat ggtgagttat
      601 ggtcaggcgg tttcctcatg gaatcagtat gctgatgcca ataatctcac cccggaacag
      661 aagcaggcag gactggataa acttgccaaa ggtgagttac ctgaaggtgc caatataagt
      721 aaagtgatag ttgatggcta taaagatggc gtattgattg caggtgcctg gtatttagga
      781 cctgcagcat caacaggtaa agtagtcagt ggcgcagtca tctcagccat tgccaatggt
      841 acgtatcagt ggtacgactt gagcaaacca ggcaatgaaa acaagagctg ggattactgg
      901 gaagtgcctc ggcaggcatt actggagcgc tggctccagg tcgaaatata tggcagaacg
      961 cagggatagc ggcaggtgga actctattta ttgatggacc agataaaggt gctttaactg
     1021 gtacgggagc tggttgggta ttcggtactg tagttggggt agttgcaccg ccggtattta
     1081 gccctgtttt aggtccaggt tcagtgcctt caggtgatat tattggtgct ataggtggtg
     1141 aatttatcag caatgcagtt aaagatgaga taaatggaac gaaaaaataa agctactgta
     1201 ggtttactgc tcctaagtat tatgtacatg attttctgct gcttaatatt cttcttcttg
     1261 actctgatag tagttaaatt tgttattagc catgaagtga atattaagca atcagatctt
     1321 gaaaatattt tagttgctag tgttattgtt ggaactgcag cagcatttag atcgtggatc
     1381 ttcaccaaat tggacgaacg caaggctcga aaaacgccct cgtcaggatc ctgagtaatc
     1441 cggacattgg gaaataacgt tcttgctggt gccgcaggtg ctgccaccgg cgaggtggtg
     1501 ggaatgatag ccacacagat gtacggtaag ccggtgagtg aactgagtga gacagagaag
     1561 cagacggtct cgacactgac aacggttgct gccggcctgg ccggtggtct ggtgggagac
     1621 tcgggggctt cagcggtagc cggcgcacag tcgggtaaga caacggttga gaataatctg
     1681 ctgggtggca gtgaattgct ccagacggaa aaagccaggg agcatggtgc tgatgttctc
     1741 tcctgtagtg ataatccttc cggtgaagcc tgtaagcggg ggcaggctga aaataaagct
     1801 tatgcagccg cattagcaac aggcagcgtt gccctgttac cggggagtgc ccaggctatg
     1861 tggttacttg gtgccgggac aaatgccggg atgcagtatg cagatagtgg tgaaataaac
     1921 ccggtcaact cagtggcagc aggctggata aatgtgataa ccatggggca gggctggaaa
     1981 gggacaatag cttggaatgc ggcaggtgga gcattaacca acgccataaa cggagatgat
     2041 cctcttactg gggcgattac aaatggagcg ggggccgggt ttggatatgg ggttggtaat
     2101 tatgtggtca agcctgctgc aaatacaatc ggtaaatgga taaccggtgg ctggaatcct
     2161 aaattcgatc cgactctact aaaatatact gaagttaaag ggcagttagg tctatctaaa
     2221 gaaatggtac cgagtaagat tccgggggct gcaggtaata tgggggcttc tattacatca
     2281 gaaatgacgg ggactgaact acaaaaagtc attaatgata aattgaagaa aggagagcgt
     2341 gaatgaaact atttattttc agtctctttg tcatagttac c
//
LOCUS       DAZFWL020000306         2379 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFWL020000306 DAZFWL020000000
VERSION     DAZFWL020000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.310"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1013
                     /locus_tag="ACHKNP_RS25865"
                     /old_locus_tag="pgaptmp_005173"
     CDS             <1..1013
                     /locus_tag="ACHKNP_RS25865"
                     /old_locus_tag="pgaptmp_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion protein GogB"
                     /protein_id="HGU4707129.1"
                     /translation="EGIECLTVCHCHISGVPESVRHLEIRGYATDGIKNVPNGLLSLS
                     INRYTPENQARIDSLISPSLQTLSLTGCSNIILPEKLPESLRSVTIHVEQKTTWNIVA
                     EGVPDGLDLDLQNVLLSPNVIKEKNITFQGNALDAALHFRPGDIVYGLSSPREKLVNS
                     IKLVNDFSKKDIVIQNTLTNAVWDARTPRKYKQDSLIKRALNEHERGTKFKQCLKNHN
                     NYNVIMADLSIYNRDKLWAKTSKAGLEFQTLIRNRTVMFCADGLVNSLKLIANKSEGY
                     GQSITASELRWIYRNKDNSQIMKNIKFYLHGKEIPAERILDTPEWKDYRPKYSGSTYK
                     YS"
     gene            complement(1390..>2379)
                     /gene="espN"
                     /locus_tag="ACHKNP_RS25870"
                     /old_locus_tag="pgaptmp_005174"
     CDS             complement(1390..>2379)
                     /gene="espN"
                     /locus_tag="ACHKNP_RS25870"
                     /old_locus_tag="pgaptmp_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspN"
                     /protein_id="HGU4707130.1"
                     /translation="EKNINDNIIYHPSNNANFYSVIESIDGNDFIYNEKQSKILEMKQ
                     DRGGNRYSAVDLNSSKYGYYEKVGGGFYRYIESFNPISSETPNKIVYKGESVDLTKEP
                     NSELYSGRYSINNKQVNVYFFRDADGTFYKSEGLHGGGVIRYIDKPYSQLREGDIGYD
                     EDLLDIYDDSPVLEDTLPALSSEIVPTPEHSIKQIYSKIKEGHIELSDSDIILCRGTT
                     GIQAENIVEYKTAGGLPDSNPNVKAPDEYMAQQQVRIGRILPEYTSDLSVADRFSREH
                     YLIVVKVKAKYITRGSVTESGWVIDKTAPVEPLAIIDRTFGMKENISMVNASK"
BASE COUNT          769 a          499 c          420 g          691 t
ORIGIN      
        1 ctgaaggcat cgaatgcctg acggtttgcc actgccatat atctggagtg ccagagagtg
       61 tccgtcatct tgagatcaga ggttacgcca cagacggcat aaaaaatgtt ccaaacgggt
      121 tattatctct cagcatcaat cgctacaccc cagaaaatca ggccagaatt gacagcctga
      181 tatcaccgtc actacagacg ctatctctga ctggatgtag caatattata ctgccggaga
      241 aactcccgga gagtctgaga tctgtaacca ttcatgtgga gcagaaaact acatggaaca
      301 tcgtagctga aggggttcct gatggactgg atcttgattt acaaaatgta ctactctctc
      361 cgaatgtaat taaagaaaaa aacatcactt ttcagggcaa cgcactggat gctgccttgc
      421 attttcgtcc gggagacatt gtctatgggc tatcctctcc cagagaaaaa cttgtcaaca
      481 gcatcaaact ggttaacgac ttttccaaaa aagatatcgt aattcagaat acattaacaa
      541 acgcagtatg ggacgccaga accccccgta aatataagca agactcgctt atcaaaagag
      601 cattaaatga acatgaaaga ggaacaaaat ttaaacagtg cttaaaaaat cacaataatt
      661 ataatgtcat catggccgac ctttccatat acaatcgaga caagttatgg gcaaaaacaa
      721 gcaaggcagg cctagagttt caaacattaa ttcgcaacag aacggttatg ttttgtgcgg
      781 atgggcttgt caattcactc aaactcatag ctaacaagtc agagggctat ggtcagagta
      841 ttaccgccag cgaattacga tggatttatc gtaataaaga caacagccaa ataatgaaaa
      901 acataaaatt ttatctgcat ggcaaagaga taccagcaga aagaatatta gatacaccag
      961 agtggaaaga ctaccgtcca aaatactccg gttccacata taaatattct taatgatagc
     1021 aaaaaatata ttttcgatat aatcaatgtt atgattaaag agtatttcat cagggcaggt
     1081 aaaaacagag taaatcagca aaagaagctg atcttcagcg atactgacac taactgacgg
     1141 tttaagcggt cgtatgaagc agcagctttc cgacggactg ccatgcggat cgtttacctt
     1201 ttgggctatt ccgcccgtca tcaagcggct cacgagtact gagtttatca ggatgatatt
     1261 gctgacaatg gtaattcgtt gaccgatgta tacttcacta tatatcggtc aacaccgtac
     1321 tgccgtgcaa catctcaaag atgtagcatc gtttttacca gaggaggcag gacatagatt
     1381 gtaaaaaaac tatttcgatg catttaccat tgagatattt tccttcatac caaaagttct
     1441 atcaattatc gcaagtggtt caacaggtgc ggtcttatct ataacccaac cactctctgt
     1501 aacacttcct cgtgtgatat attttgcctt tactttaaca actattagat aatgctcacg
     1561 actaaaccga tcagcaacgc taagatccga tgtgtattca ggcaatattc ttccaatacg
     1621 tacctgctgt tgtgccatat attcatctgg tgcttttaca tttggatttg aatcaggaag
     1681 ccctccagca gttttatatt caacgatatt ttcagcttga ataccggttg tgccgcgaca
     1741 caatatgatg tctgaatcgg acagttctat gtgcccctcc ttaattttcg aataaatttg
     1801 tttaatacta tgttctggag ttggtactat ttcagaagat aaagcaggca acgtgtcttc
     1861 aagcaccgga gaatcatcgt atatatccaa caaatcctca tcatacccaa tatctccttc
     1921 tcttaactga gaatacggtt tatctatgta tctaataact cccccaccat gaagaccttc
     1981 tgatttataa aatgtaccat cagcgtcacg aaagaaataa acattaacct gtttgttatt
     2041 tatagaatac ctacctgaat ataattccga atttggctcc ttagttaaat ctactgattc
     2101 ccccttgtag actattttat ttggtgtctc tgaagatatg gggttaaagg attctatata
     2161 tctataaaaa ccaccgccaa ctttctcata atacccatac ttagaagagt taagatctac
     2221 tgcactatat ctattccccc cacgatcctg tttcatttct aatattttag attgtttttc
     2281 gttatatata aagtcattac catcaattga ctcaattaca gaatagaaat tagcattatt
     2341 actcggatga taaataatat tatcattaat gtttttttc
//
LOCUS       DAZFWL020000307         2366 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFWL020000307 DAZFWL020000000
VERSION     DAZFWL020000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.311"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1164
                     /gene="iha"
                     /locus_tag="ACHKNP_RS25875"
                     /old_locus_tag="pgaptmp_005175"
     CDS             <1..1164
                     /gene="iha"
                     /locus_tag="ACHKNP_RS25875"
                     /old_locus_tag="pgaptmp_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HGU4707131.1"
                     /translation="ENKGRELVRSVLKRDKWGLAGQPRELKESNLILNSLLLTPLGES
                     HLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHLTDALALTAGSRYEHHE
                     QFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLHKGISGVSGQGKTNLLGNPD
                     LKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSYSINDNTNSYVNSGKARLHG
                     VEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVA
                     SWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKMTDALTLNA
                     AVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
     gene            complement(1382..1532)
                     /locus_tag="ACHKNP_RS25880"
                     /old_locus_tag="pgaptmp_005176"
                     /pseudo
     CDS             complement(1382..1532)
                     /locus_tag="ACHKNP_RS25880"
                     /old_locus_tag="pgaptmp_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1744..2064)
                     /locus_tag="ACHKNP_RS25885"
                     /old_locus_tag="pgaptmp_005177"
                     /pseudo
     CDS             complement(1744..2064)
                     /locus_tag="ACHKNP_RS25885"
                     /old_locus_tag="pgaptmp_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
BASE COUNT          624 a          541 c          608 g          593 t
ORIGIN      
        1 gaaaataaag gtcgtgagct tgtacgcagt gtactgaagc gcgacaaatg ggggcttgcc
       61 ggtcagccgc gggagcttaa ggaatcgaac cttatcctga attcattact gcttacccct
      121 ctgggagaat ctcatctggt tacggtgggg ggcgagtttc agagctcgtc catgaaagac
      181 ggagttgtcc ttgccagcac aggtgaaact ttccggcaga aaagctggtc ggtatttgct
      241 gaggatgagt ggcatctcac ggatgcactt gcgctgactg cgggcagccg ctatgaacat
      301 catgagcaat tcgggggaca cttcagtccg cgtgcatatc tggtctggga tgtggcagat
      361 gcctggacgc tgaaaggcgg tgtgaccacg ggatataagg cacccagaat ggggcagcta
      421 cataaaggga ttagtggtgt gtccgggcag ggaaaaacaa atctacttgg taaccccgac
      481 ctgaagccgg aagagagcgt cagttatgag gctggggtgt attacgataa ccccgccggt
      541 ctgaatgcca atgtcacagg ttttatgact gacttctcca acaagattgt ctcttattcc
      601 ataaatgata acaccaatag ctatgtaaac agcggaaagg cccggttgca cggtgtggaa
      661 tttgccggca cattgccgct gtggtcagag gatgtcacgc tgtcactgaa ttacacctgg
      721 acccgaagtg aacaacgtga tggtgataac aaaggtgcgc cgctgagtta tacccctgaa
      781 cacatggtga atgcgaaact gaactggcag atcaccgaag aggtggcatc atggctgggt
      841 gcccgttatc gcgggaaaac accacgtttc acccagaatt attcgtcact gagcgctgta
      901 cagaagaaag tgtatgatga gaaaggagaa tacctgaaag cctggacggt ggtggatgca
      961 ggtctgtcgt ggaagatgac ggatgccctg acgctgaatg ctgcggtgaa taacctgctc
     1021 aacaaggatt acagtgacgt gagcctgtac agtgccggta agagtacgct gtatgccggt
     1081 gattacttcc agacgggatc atcaacaaca ggatatgtga tacctgagcg aaattactgg
     1141 atgtcgctga actatcagtt ctgataataa caaaacgcta tcactgacgg tagaatacgt
     1201 tgccactgca actcctggcg gaacagtggc aacgtattag gttaagtgca tttccgatcc
     1261 gctaatgaga tttcgttacc aacaactaat atcgtcacag gaaatgcacg gattattttt
     1321 aacttatcat ttacatactt gtccagagtg taagcgcacc gtgaaggacg tggggtaaaa
     1381 attagtttac agagagagtg acgttccagg gcaacaactc tttcatgcgg ttggcaggcc
     1441 aggtgttgat tacactgatc acgtggcgta gccacgtttc cggctcgatt ccgttaagtt
     1501 ttgcagctac cgatcaggct gtacatcact gccgcactat cgctcgtcat ctcaaagtcc
     1561 ggtctcgtca gcaggaaggt atcattctct cccgccattt ttccaggggc ccggtcagat
     1621 aagtcccttt gtctatcgct gactcctgac tcataacccg gttagcagaa tgcagggtca
     1681 ccacctcgcc acgaccaaat ccaaataagt caattgcacc ttctcaatcg ccattttgtc
     1741 agtaagcgta cagcctgaac tgatggtatc ttcaccatca atgacaacgg tgatcgcaat
     1801 tttactgacg ttcgccggaa cacgatccag tgctatctca atgctggcct gctgcgaacc
     1861 ggtaacgagc ctgactgccc cctcaggaga agacaaatta ttataaaaga taaagtcaga
     1921 atcgccactg acctttccct gagcattaag catgaacagg caggtatcgg gttcgccttt
     1981 aaagccggat ttggctgggt actgaagatt cagcctgatc gtagattgct gaaggggtat
     2041 gttttgtccg gattgtaaat tcatattaac tctcctgatt tttgattatt attaatgcgc
     2101 agcgtttata tatgttccct aggcttagtt ctggacgctg gatattcggt gaggcgtaaa
     2161 tatggtatga caccattttt cataacgctg aagtttctat acctgttgaa tttgaatttt
     2221 cattgaccgg gtatcttatt ttccagggcc cgttccttca taagtcgcaa aagtaacata
     2281 tatccgaagg gcatgctgtt gatatcagac acggaatact ggctttaacc agcaaccaca
     2341 gataaacccg gggcctgcat aaaagg
//
LOCUS       DAZFWL020000308         2359 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFWL020000308 DAZFWL020000000
VERSION     DAZFWL020000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.312"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..587)
                     /locus_tag="ACHKNP_RS25890"
                     /old_locus_tag="pgaptmp_005178"
     CDS             complement(<1..587)
                     /locus_tag="ACHKNP_RS25890"
                     /old_locus_tag="pgaptmp_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4707132.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVRVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NITQGSGKEWSKQDRDTFDPTHDLDLYCDQASGLV"
     gene            complement(645..992)
                     /locus_tag="ACHKNP_RS25895"
                     /old_locus_tag="pgaptmp_005179"
     CDS             complement(645..992)
                     /locus_tag="ACHKNP_RS25895"
                     /old_locus_tag="pgaptmp_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU4707133.1"
                     /translation="MVTKTITEQRVEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(1029..>2359)
                     /locus_tag="ACHKNP_RS25900"
                     /old_locus_tag="pgaptmp_005180"
     CDS             complement(1029..>2359)
                     /locus_tag="ACHKNP_RS25900"
                     /old_locus_tag="pgaptmp_005180"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4707134.1"
                     /translation="EYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTGY
                     DGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTAC
                     SAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGNQ
                     FEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLAD
                     ELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLAT
                     MLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPEM
                     TVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLAA
                     GSGKAEPVSASTTAMFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
BASE COUNT          466 a          754 c          602 g          537 t
ORIGIN      
        1 ttcacaagac cgctggcctg gtcgcagtag aggtcaagat catgtgtagg atcgaacgtg
       61 tcacgatcct gttttgacca ctccttacca ctaccctgag tgatgttatt cttcgtcgac
      121 ctgccaaaat cgacctcaat tttctcgaac tggtctcctt ccatcgtgta tttgccatac
      181 agcacggcat tcaccgcctg catttcttcc acccggacaa tggcgtgctc ttcctgtttg
      241 aggttatcag tgatgatacg cagacgacgg taggccgggt cgttcagctg agccggatct
      301 tcaccgggaa gacgctcaac cgcctgctgg taattaaatt cgtgtttcgg cttgacgtag
      361 cccggacgta acacgcgggt ttcaccacca cgatgacgca gcacttttcc ttcaacaacc
      421 ggggaaacat aggccgccac cggcgttttt ccggtaattt tgtccagcat cacctcttcg
      481 gtatggaaat tcaccgtacg gcggaaaaac agctccagaa acagcgcacg aaatttcact
      541 ttttgttcgg tataaccgag taactggcgg gtcgtaaaca atcccataaa tcagttcctt
      601 tcattcagaa atcagtcagg ccaccgcggt ggcctgataa cgtgttacgg cagcgccgcg
      661 tgactcaggg cactgccggc aaaggcgttg gcctttttgt gttcatccac actttcaggc
      721 cagcggattg cctccgtcgc aaaggtcccc gacttgtaat aggtcagcgc cgtctctgtg
      781 ccttcaagcg gcagtaccag tatgccaacc gcactaccgg ctttctgtcc gtcccagacc
      841 accagtttcc cggtggcctc atccagcatc aggggcgtca gtgccggtgt tgccgaggaa
      901 atcccgctgc tgcctgtggc ggtatgagcc ggatcattac cggcaaaaat acgtacttcc
      961 acacgctgtt cagtgatagt tttcgtcacc atattgtaaa aacctcctgt tgatggtcag
     1021 cactggcttc atggcatggc catgagcatt ttcacgtccg catcaccgtc tgctgacgtc
     1081 tgtggcacgc caccctgtac cgctgccggt gaatggttcg ccatgatgcg ttcaaacatg
     1141 gcggttgtgg atgcagagac cggttctgcc ttacctgatc ccgcagccag cacagcccgg
     1201 gcgctctcca cagtcattcc cgggcaggca gccagctgtt cagcctgcgc ctcagcccct
     1261 tttgcctcat ccagtgccat gatctgatca cggagtgagg gtccggcatc cgcctgcggt
     1321 gaagcagcca ggatcgggcg ggctttttcc accgtcatct ccggcatcgc cgccagcgtt
     1381 gccgccagtt gttcacgacc tttcgcttct tcacacgcca taatgcgatc ggcttcactc
     1441 tgcgcggatg ccaccggctg ctgtggtgcc gccgcggcca gaatcgcccg ggcctgttca
     1501 acgctcatgc cctgttgtcc tgccagcatc gtggcaagct gttcacgtcc tttcgcttcc
     1561 tggcatgtca ggatccccat cactcgctgg ttctcctgcg cggcggcttc cgttgcagtt
     1621 aattgcggca tagtgcctcc tctgacatta ctgttcagcg ccgtggccat cacactgatg
     1681 gcatccgacg cattgattaa ttcatccgcc agcccggcct caatgccgga ctgaccttca
     1741 aaaacggcgg cctctgttcc cgtgacagca tcaacagaca gaccggtata catcgctact
     1801 ttttcagcaa acatccggcg cgccgcatca atgcgctgtt gcatgtcctg gcgaacctct
     1861 gccggcaacg cttcaaactg attgccatcc accttgtgcg cccctgagta aatcagcgtg
     1921 atatcaacac cggcctgcgc cagatgaccg gcatagctga catggctcat catcacgcca
     1981 atggagccga tacgggatgt ctgggtaacc agccgtcggg agcaggccga cgccagcagc
     2041 atggctgcag agcaggccgt gtcattgcac agtgcccaga ccggcttctg ctgacggagg
     2101 cggtaaatca tgtcagcgca gtcaaacgcg ccggcggcct gcccgcccgg actgtcaatg
     2161 tccagcagta cgccccgcac ctggctatcc gccattgcct gctgaagaca ggcgacaatg
     2221 ccgtcatagc ctgtcattcc ggaaaatggc cgcatacccc ccagccggtg caccagcgtg
     2281 ccggtcaccg gcagtacagc aataccgttc accacccggt aaacacgggc cggtcgttta
     2341 cctccggcca tgtactcgt
//
LOCUS       DAZFWL020000309         2353 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFWL020000309 DAZFWL020000000
VERSION     DAZFWL020000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.313"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..678
                     /locus_tag="ACHKNP_RS25905"
                     /old_locus_tag="pgaptmp_005181"
     CDS             <1..678
                     /locus_tag="ACHKNP_RS25905"
                     /old_locus_tag="pgaptmp_005181"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4707135.1"
                     /translation="PPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGNVG
                     RAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRG
                     LIVLTDRYPQDQISGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLN
                     VDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAIMDF
                     MTARGYH"
     gene            complement(727..2226)
                     /gene="pitB"
                     /locus_tag="ACHKNP_RS25910"
                     /old_locus_tag="pgaptmp_005182"
     CDS             complement(727..2226)
                     /gene="pitB"
                     /locus_tag="ACHKNP_RS25910"
                     /old_locus_tag="pgaptmp_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HGU4707136.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVSNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            2163..>2353
                     /locus_tag="ACHKNP_RS25915"
                     /old_locus_tag="pgaptmp_005183"
     CDS             2163..>2353
                     /locus_tag="ACHKNP_RS25915"
                     /old_locus_tag="pgaptmp_005183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707137.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTI"
BASE COUNT          636 a          602 c          572 g          543 t
ORIGIN      
        1 cctccattaa ttgccgttat tggtagcgat ggttcaggca agtcaacggt gtgtgaacat
       61 cttattaccg ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg aaaacaagcc
      121 ggaaatgtcg gtcgtgcagt gacaaaatta ccgttgatgg gaaaatcctt acataaaaca
      181 attgaacgaa atcaggtgaa aacagcaaaa aaattgccag gaccagttcc ggcgctggta
      241 attacagcgt ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc ctgtcgtcgt
      301 cgcgggttaa ttgttctaac cgaccgttat cctcaggacc aaatttctgg cgcttacgat
      361 ggtacggtgt tcccacctaa cgttgaaggt ggtcgttttg tctcatggct ggcaagccag
      421 gaacgtaaag cgtttcactg gatggcgagc cataagcctg atctggtcat caaactcaat
      481 gttgaccttg aagttgcctg cgcgcgtaag cctgaccaca aaagggagtc gctggcaaga
      541 aagatagcca taacgccaca gttaaccttt ggtggtgcac aactggttga tatcgatgcc
      601 aatcagccac tggaacaggt gttggttgat gcagaaaaag cgattatgga ttttatgacc
      661 gcgcgtggtt atcactagcc aaaaatggaa atgcccgatc gccaggaccg ggcattttca
      721 ggaaggttaa atcaactgca atgctatcca gtacagccca ccagaaagaa aaatcgccgc
      781 cggtaaagta aatacccacg ccatcaggat gctggttacc gttttacgct gtaacccacc
      841 gccgtccacc accatcgtcc ctgcaactgc agacgagagg acgtgtgttg tggagacggg
      901 catcccaata taactggcaa gaccgataga cactgccgcc gtcatttgtg ccgccatgcc
      961 ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg atggtcatcg ctacacgacg
     1021 ccagccaatc atggtgccaa tgccgagcgc cagtgctacc gccatgatga tccacaccgg
     1081 agcgtactca atggtgctta acatatcgct gcgcagtttt ttcagcaggt tctggtcttc
     1141 tttactgacg cctggcagtt tcgccagctt cgcggaggta tctgagatgc acagcataat
     1201 gcggcgcagc tggctgcgct gactcacgct taacggctcg taactttcca tattgcctgg
     1261 cagcatcgtt ttaacgcgcg caatcgcatc aaaggtattt gccggatgac agtgaaactc
     1321 tgttacttgc gcgccatcag tcgatgccgc aggcaatgga ggttccatcg caatcaactt
     1381 ctgcggcagt tcaggatgct gttgcagata gtgttcgaag ttggagacgg catcgcgagt
     1441 acgggtaatt tcatagccgg acgcgttcat attgacgacg aagccagcag gggcaatccc
     1501 caccagtacc agcatcacca ggccgatccc tttctgcccg tcgttcgcgc cgtgcgaaaa
     1561 cgccacgccc gcagcggaaa caatcagcgc aatacgcgtc cagaatggcg gtttacgttt
     1621 gccttttttc tttttgcgat cttccggaat gcggtgaata cggtcacgct ttttcgtccc
     1681 gctccagaaa cggcgcagca ggaaaatcag accacccgca atgaccaggc cgacgatagg
     1741 ggaaacaatc agcgaggaga aaattttggt cacttcacgc aggtttaacg catccatcac
     1801 cgatgagccg gttaacagcg cgttggttaa accgatgccg ataatcgcgc caatcaaggt
     1861 gtgcgaactg gaggccggta aaccgaagaa ccacgtcccc aggttccata taatcgccgc
     1921 cagcagcatg gaaaagacca tcgccagacc gtgggttgac cccatattca gcaacaaatc
     1981 ggttggtaac atatggacaa tggcataggc aacgctaagt ccgcccaata acacgccaaa
     2041 aaagttaaaa aatgccgcca tcaccacagc aagttgtggt tgcatggcac gagtataaat
     2101 aacggttgcc accgcattcg ccgtgtcatg aaaaccattg attgcttcgt agaacaacac
     2161 aaatgccaga gcaagcaata acaaaagccc tgtgtatata tcaaggccaa caaataaatt
     2221 tagcatatat tagattacgc cattttgaat ttacgaacgg acgcattatc agtgacttta
     2281 acggcatggg caaagtgaaa tatcattttt taacgataaa aagagtgtta tttcttgtgt
     2341 atcagacaat aag
//
LOCUS       DAZFWL020000310         2351 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFWL020000310 DAZFWL020000000
VERSION     DAZFWL020000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.314"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            488..715
                     /gene="ykgL"
                     /locus_tag="ACHKNP_RS25920"
                     /old_locus_tag="pgaptmp_005184"
     CDS             488..715
                     /gene="ykgL"
                     /locus_tag="ACHKNP_RS25920"
                     /old_locus_tag="pgaptmp_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HGU4707138.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(750..890)
                     /gene="ykgO"
                     /locus_tag="ACHKNP_RS25925"
                     /old_locus_tag="pgaptmp_005185"
     CDS             complement(750..890)
                     /gene="ykgO"
                     /locus_tag="ACHKNP_RS25925"
                     /old_locus_tag="pgaptmp_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HGU4707139.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(890..1153)
                     /gene="ykgM"
                     /locus_tag="ACHKNP_RS25930"
                     /old_locus_tag="pgaptmp_005186"
     CDS             complement(890..1153)
                     /gene="ykgM"
                     /locus_tag="ACHKNP_RS25930"
                     /old_locus_tag="pgaptmp_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU4707140.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVTSEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1517..1618)
                     /gene="ykgR"
                     /locus_tag="ACHKNP_RS25935"
                     /old_locus_tag="pgaptmp_005187"
     CDS             complement(1517..1618)
                     /gene="ykgR"
                     /locus_tag="ACHKNP_RS25935"
                     /old_locus_tag="pgaptmp_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HGU4707141.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2092..2181)
                     /locus_tag="ACHKNP_RS25940"
                     /old_locus_tag="pgaptmp_005188"
                     /pseudo
     CDS             complement(2092..2181)
                     /locus_tag="ACHKNP_RS25940"
                     /old_locus_tag="pgaptmp_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          689 a          441 c          522 g          699 t
ORIGIN      
        1 cttgcatttt atattggtca ggaactttgc cctgaagtgt taggaatagt cttaatgcgc
       61 tgtgtgatgg actagtaaaa aaacaagttt taagggtaag ggatagaata taaaactata
      121 atgagctgtc attgaagatg atttgccaat cagtaagaaa ttttaatgga ttaacatgcc
      181 gcacaaaatg gttataggtg ttcattacaa taagtgatgg aaaattgaac gggcgtatgt
      241 acgtcatctg ttcggtaagc ccccaagagc cagattaagt gaagttaaac gaaggctgcc
      301 agctgtcaca ggaacgatgg cctctttgtc agggcactga gtgcagttat ctgtgcgcgt
      361 tactctaact ggcactcgaa cgtcggctac gggttaccgg tggatcagac tgttcctgtg
      421 gcgtaaacgt ttttatgtta gtaccaacag atttaccaca tttatcatct taacagtttg
      481 agtgaaaatg caggctgcgc cgcaaaggga ggagactgag tggcgtgttc agtctaaaag
      541 agggttgatg ccagcataca ggggcgaagc tggtcaacaa gtcaacatta agataatgga
      601 atattcggag agaaacgtca ggcagttggc ctcaaatgaa caggaagaat atatccccag
      661 gaaaattaat gttggtgtta taaacacacc aacattaatc cgatctgatt attaatcaga
      721 caaatatcgt taattcgtcg aattttgaat caacgttttt tcttacgacc ctgaacggcc
      781 ttaaaacgtg gattagattt acaaatcaca tacaaccgtc cttttcgctt cacaatctga
      841 cagtctggat ggcgttcttt tgcggtacgc agagagttaa gaactttcat cacgccccct
      901 ttttcgtgct aacaaaacga ccaaaacgtt gggtgaatcg tgcaacattt ccttctgatg
      961 tcactgttct cagcttccct gtatagaacg ggtgcgattt agaagagaca tcaattgtca
     1021 cgtacggata cgttacgcca tccagctcaa tctcacggtc tgttttgata gtcgagccga
     1081 ttttaaagta ctcatcaaca ctggtgtcgt ggaacaccac agtacgatac tcaggatgga
     1141 tattgggctt catcattttt acctgttatg ttataacata accgtaaaga tacatgctca
     1201 tccggatcat ttcaacaccc aatccctgtg cccatttgtg gtatgtggaa tttccccaac
     1261 ggggaagtct ggcattgtta gcagccgttg gcgacgctcc cataaacgcc tggatgagtg
     1321 attgatgatg gttttgctga gagtatggat ttttcgagaa tggaggttat ctccacttta
     1381 ctggccgatg atttatcgca gtatgattga ttacctgaca gcgtgtattc ctctcccacg
     1441 gatataataa ttacttacaa aaagggagag gatgcatatt ttaaatatca ctgaagtgaa
     1501 cagtttattg ccgttattaa tagaaatgga gaaataaata ggcgtattct acaattgcga
     1561 caaaaacaac gatattaatc agtttatgac tgatttgttg tactttattc tctttcattg
     1621 gtacttcctc gctttaaaaa agagtgcact tcgtaagtgc ccttatataa ataacgagtt
     1681 tggtcaacca attttttgac atgtatcaca aatttgaata gatgtattac atcagctatc
     1741 ttttattgca ccaacgtcat tgatatatgt cgactgaagt cagttccggg aatgagtctg
     1801 atctcaagac tggcccagtc cgggcgttga ttggcgctga ggagcatacc tcatttcatc
     1861 ataatgtcgt atctcctggg gtgttataca agatatcgtt gttggtaacc tgggaaagga
     1921 attgagttct attaaaccgt caactatgcc ggatacatac tggattacac tgcaggcacg
     1981 ccttatgaga gaacgtgccg cagtgacggg ttaattatct gaaagaattt gtgaggctgt
     2041 atcggttact cattgatttg atagttttac tctcgggaga ataatagata tttaatccat
     2101 taacggaaac cagccagttc ctttcgatgc ctgaatttga tcccatagtt tatccggaat
     2161 ggttaattca tgaacaagtt gtgttatcgt tcatgagaag cataacgtaa agggaaaagc
     2221 tcgattagac ggcagaattt gtcaggggtt atgaacgaaa ttcataaatc tgtttgagtg
     2281 ttgcgatggg tagtgcaagt tcgatatctc cgcaatttac agtccgatga aggaaaatga
     2341 atatccataa a
//
LOCUS       DAZFWL020000311         2274 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFWL020000311 DAZFWL020000000
VERSION     DAZFWL020000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.315"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..355)
                     /locus_tag="ACHKNP_RS25945"
                     /old_locus_tag="pgaptmp_005189"
     CDS             complement(<1..355)
                     /locus_tag="ACHKNP_RS25945"
                     /old_locus_tag="pgaptmp_005189"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGU4707142.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLL"
     gene            1041..1331
                     /locus_tag="ACHKNP_RS25950"
                     /old_locus_tag="pgaptmp_005190"
     CDS             1041..1331
                     /locus_tag="ACHKNP_RS25950"
                     /old_locus_tag="pgaptmp_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU4707143.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            1355..1606
                     /locus_tag="ACHKNP_RS25955"
                     /old_locus_tag="pgaptmp_005191"
     CDS             1355..1606
                     /locus_tag="ACHKNP_RS25955"
                     /old_locus_tag="pgaptmp_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU4707144.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(1654..>2274)
                     /gene="traT"
                     /locus_tag="ACHKNP_RS25960"
                     /old_locus_tag="pgaptmp_005192"
     CDS             complement(1654..>2274)
                     /gene="traT"
                     /locus_tag="ACHKNP_RS25960"
                     /old_locus_tag="pgaptmp_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HGU4707145.1"
                     /translation="QYTSTIWLEPASERTVFLQIKNTSDKDMSGLQGKIADAVKARGY
                     QIMTSPDKAYYWIQANVLKADKMDLRESQGWLSRGYEGAAVGAALGGGITAYNTGSAG
                     TTLGVGLATGLIGMAADAMVEDINYTMITDVQIAERTRTSVRTDNVAALRQGTSGSKI
                     QTSTETGNQHKYQTRVVSSANQVNLKFEEAKPHLEDQLAKSIANIL"
BASE COUNT          617 a          477 c          495 g          685 t
ORIGIN      
        1 aaagtaataa aactccccat ataatgatag caactaaaat cgtaatcaac actgcggctg
       61 aacccatatc ttttgctctg cctgataatt cgtgatgttc tgaaccaatt ctgtcaacaa
      121 ctgcttcaat tgcactgtta attatttcaa caataattac catcattgcc gaaccaatga
      181 gtaatactcg agtaacagag tcaacatcca gccaaaagga tattatcagt gctgacagaa
      241 ccagaatgca ctcctgacga aaggctgctt catagcgcca tgccgcacat aagcctttcc
      301 atgaataacc cgctgcttta ataattctgg taacaccagt ggtcgtatta gccataaata
      361 cctcttattt aattatatgt ctgaatatgc attataaaat atcagttaac attaacagga
      421 ttttgtaata agccacattt attctttcgg gaattaaaaa acataacgaa attttgcctc
      481 attctgcatt tcattgaaat agtatgtttc tcatcgaaag caagtttgtc aatataaatt
      541 tgtaacagaa atgactttcc ggggataaac aaaactgttc atggcaatgg ctttctctgt
      601 cgccaacatg gatacattgt tccggataaa gcactgatgt cattgactga cgcagactat
      661 caggccatct gatgctttct tttccttcac cggtattgat gatatgcctt atgaaggggg
      721 acttattcct gtaacccaat cacaggcact gtgattactg gtttatgatt gatgctgatt
      781 tgagcagata tgcacagcag ttgtggattc aggggatatt ttctgccaga tattcatacc
      841 agattgatga cctctttgct cagatgctct tcgttctgag ccagccagga ttttgtggcc
      901 acatacattg ttcgccgatg gggaatcgag acaggcgatt ggctgtgatg cgattttgcc
      961 gctaactgag gatgagtccg cccctcccgg ggaattcctg taagttaagc cgtacctgtt
     1021 gattagcact aaactgggta atgaatttat ctgccagtat gaatgatgcc ggggacggtt
     1081 ttgaactcct gatgtcgggt aatatgcagg attttaatat ttgttgtcgt cgggataatc
     1141 attatcctga acataaactg atacctttat cagagttatt tgacgttaat tatggactga
     1201 atcttgagct gaataaactg gagaaagatt cttcaggaat taattttgtt tcaagaacgt
     1261 ccaaaaataa tggcgtctcc gccagggtta aattaaaaga tggtattagt ccatgccagc
     1321 agctgtgtta actgtagcag caggaggttc tgttatggaa tcgtttgtac aggattcacc
     1381 attttatagt ggaagagatc tttattggtt acgtcctaaa gttgaactga ctctggaaga
     1441 gaaattgtac tactgctctt gtataagaag aaacagacac aaatacagct acgggcgtca
     1501 ggcaaaccga acgctgaaga atttactggt accgtcatta gattctgttc cggcatgggt
     1561 atatggtgtg acagggaaaa taatatctga attgtctgaa cgttgagact aagagggccg
     1621 gaggccaggt gaatggccac cgggatattt atatcagagt atattggcga ttgattttgc
     1681 cagctggtct tccagatgag gtttagcttc ttcaaacttc agattaacct ggttggcact
     1741 ggatacaacc cgggtctggt atttatgctg gttgccggtt tctgtgcttg tctgaatttt
     1801 ggagcctgat gttccctgac gcaaagctgc aacattatcc gttctgacgc tggttctggt
     1861 tcgctcggca atctgaacat ctgtaatcat tgtgtaattt atatcttcca ccattgcatc
     1921 cgcggccatg ccaatcagac cggtagccag tccaacaccg agagtggtac ctgccgaccc
     1981 ggtattgtac gctgtgattc cacctccaag agcagctccg actgctgcac cttcataacc
     2041 gcgactcagc catccctgag attcacgcag atccattttg tctgctttga gaacgttagc
     2101 ctggatccag tagtaagctt tatccgggga agtcatgatc tgataacctc tggctttgac
     2161 tgcatcggca atttttccct gtagtccact catgtcttta tccgacgtgt ttttgatctg
     2221 caggaaaacg gtgcgttcgc tcgcaggttc aagccagatg gttgaggtgt actg
//
LOCUS       DAZFWL020000312         2241 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFWL020000312 DAZFWL020000000
VERSION     DAZFWL020000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.316"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>2241
                     /gene="agn43"
                     /locus_tag="ACHKNP_RS25965"
                     /old_locus_tag="pgaptmp_005193"
                     /gene_synonym="flu"
     CDS             <1..>2241
                     /gene="agn43"
                     /locus_tag="ACHKNP_RS25965"
                     /old_locus_tag="pgaptmp_005193"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HGU4707146.1"
                     /translation="TGAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPG
                     ETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSG
                     GLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQV
                     VKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINKNGRQIVRTEGTANTTV
                     VYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQK
                     GRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVVEGKADNVVLENGGRLD
                     VLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSGAAVSGTRSDGKAFSIG
                     GGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAGTTTLNNGAILTLSGKT
                     VNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTLNDS
                     TVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIPDNATVQSVVDDLSHAG
                     QIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTIL
                     NLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDESW
                     YLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGENNSVRLSIQGGHLGHD
                     NNGGIAR"
BASE COUNT          547 a          606 c          694 g          394 t
ORIGIN      
        1 acgggcgctt tcgtggttgc ctccgaactg gcccgcgcac ggggtaaacg tggcggtgtg
       61 gcggttgcac tgtctcttgc cgcagtcacg tcactcccgg tgctggctgc tgacatcgtt
      121 gtgcacccgg gagaaaccgt gaacggcgga acactggcaa atcatgacaa ccagattgtc
      181 ttcggtacga ccaacggaat gaccatcagt accgggctgg agtatgggcc ggataacgag
      241 gccaataccg gcgggcaatg ggtacaggat ggcggaacag ccaacaaaac gactgtcacc
      301 agtggtggtc ttcagagagt gaaccccggt ggaagtgtct cagacacggt tatcagtgcc
      361 ggaggcggac agagccttca gggacgggca gtgaacacca cgctgaatgg tggcgaacag
      421 tggatgcatg agggggcgat agccacagga accgtcatta atgataaggg ctggcaggtc
      481 gtcaagcccg gtacagtggc aacggatacc gttgttaata ccggggcgga agggggaccg
      541 gatgcagaaa acggtgatac cgggcagttt gttcgcgggg atgccgtacg cacaaccatc
      601 aataaaaacg gtcgccagat tgtgagaact gaaggaacgg caaataccac tgtggtttat
      661 gccggcggcg accagactgt acatggtcac gcactggata ccacgctgaa tgggggatac
      721 cagtatgtgc acaacggcgg tacagcgtct gacactgttg tgaacagtga cggctggcag
      781 attgtcaaaa acgggggtgt ggccgggaat accaccgtta atcagaaggg cagactgcag
      841 gtggacgccg gtggtacagc cacgaatgtc accctgaagc agggcggcgc actggttacc
      901 agtacggctg caaccgttac cggcataaac cgcctgggag cattctctgt tgtggagggt
      961 aaagctgata atgtcgtact ggaaaatggc ggacgcctgg atgtgctgac cggacacaca
     1021 gccactaata cccgcgtgga tgatggcgga acgctggatg tccgcaacgg tggcaccgcc
     1081 accaccgtat ccatgggaaa tggcggtgta ctgctggccg attccggtgc cgctgtcagt
     1141 ggtacccgga gcgacggaaa ggcattcagt atcggaggcg gtcaggcgga tgccctgatg
     1201 ctggaaaaag gcagttcatt cacgctgaac gccggtgata cggccacgga taccacggta
     1261 aatggcggac tgttcaccgc caggggcggc acactggcgg gcaccaccac gctgaataac
     1321 ggcgccatac ttaccctttc cgggaagacg gtgaacaacg ataccctgac catccgtgaa
     1381 ggcgatgcac tcctgcaggg aggctctctc accggtaacg gcagcgtgga aaaatcagga
     1441 agtggcacac tcactgtcag caacaccaca ctcacccaga aagccgtcaa cctgaatgaa
     1501 ggcacgctga cgctgaacga cagtaccgtc accacggatg tcattgctca gcgcggtaca
     1561 gccctgaagc tgaccggcag cactgtgctg aacggtgcca ttgaccccac gaatgtcact
     1621 ctcgcctccg gtgccacctg gaatatcccc gataacgcca cggtgcagtc ggtggtggat
     1681 gacctcagcc atgccggaca gattcatttc acctccaccc gcacagggaa gttcgtaccg
     1741 gcaaccctga aagtgaaaaa cctgaacgga cagaatggca ccatcagcct gcgtgtacgc
     1801 ccggatatgg cacagaacaa tgctgacaga ctggtcattg acggcggcag ggcaaccgga
     1861 aaaaccatcc tgaacctggt gaacgccggc aacagtgcgt cggggctggc gaccagcggt
     1921 aagggtattc aggtggttga agccattaac ggtgccacca cggaggaagg ggcctttgtc
     1981 caggggaaca ggctgcaggc cggtgccttt aactactccc tcaaccggga cagtgatgag
     2041 agctggtatc tgcgcagtga aaatgcttat cgtgcagaag tccccctgta tgcctccatg
     2101 ctgacacagg caatggacta tgaccggatt gtggcaggct cccgcagcca tcagaccggt
     2161 gtaaatggtg aaaacaacag cgtccgtctc agcattcagg gcggtcatct cggtcacgat
     2221 aacaatggcg gtattgcccg t
//
LOCUS       DAZFWL020000313         2233 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFWL020000313 DAZFWL020000000
VERSION     DAZFWL020000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.317"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            232..552
                     /locus_tag="ACHKNP_RS25970"
                     /old_locus_tag="pgaptmp_005194"
     CDS             232..552
                     /locus_tag="ACHKNP_RS25970"
                     /old_locus_tag="pgaptmp_005194"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HGU4707147.1"
                     /translation="MIAVIFEVQIQPDQQTRYLTLAEELRPLLSHVAGFISIERFQSL
                     ATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKD
                     KDNV"
     gene            545..931
                     /locus_tag="ACHKNP_RS25975"
                     /old_locus_tag="pgaptmp_005195"
     CDS             545..931
                     /locus_tag="ACHKNP_RS25975"
                     /old_locus_tag="pgaptmp_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HGU4707148.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKHEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            939..1625
                     /locus_tag="ACHKNP_RS25980"
                     /old_locus_tag="pgaptmp_005196"
     CDS             939..1625
                     /locus_tag="ACHKNP_RS25980"
                     /old_locus_tag="pgaptmp_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HGU4707149.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1603..2226)
                     /gene="tetR(B)"
                     /locus_tag="ACHKNP_RS25985"
                     /old_locus_tag="pgaptmp_005197"
     CDS             complement(1603..2226)
                     /gene="tetR(B)"
                     /locus_tag="ACHKNP_RS25985"
                     /old_locus_tag="pgaptmp_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HGU4707150.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
BASE COUNT          690 a          399 c          472 g          672 t
ORIGIN      
        1 taaataccat gcgggatccg cactattttc ggtgatagca aaaaataaaa aagtataaat
       61 aataaacaaa attataagag aaggagaaca tgataagcgc aggccgagca atttaaagaa
      121 gtgtgatata caacataaaa cagcagcatg acctttctat atttcgttgc ttatcgaagt
      181 gttaattttc gggtggatta ctctgtttgt tgattaataa cggagaaaaa tatgattgct
      241 gtaatatttg aggtgcaaat acaacccgac caacaaactc gctatttgac tttagctgag
      301 gagttaagac cactattaag tcatgtagct ggttttattt caattgaacg ttttcaaagt
      361 ctagctacag aaggaaaaat gttatcgcta tcttggtggg aaaacgaata cgcagttctg
      421 caatggaaaa atcatgtttt acatgcgaaa gctcaacaag aagggcgaga gtcaatattt
      481 gatttttaca aaattagtat tgctcatatt actcgcgaat attcatttaa aaaggacaag
      541 gataatgttt gatgttcacg ttgttttaga taatcaaata ggacaattag cattactagg
      601 aaaaacatta ggtaataaag gtattggatt ggaaggggga gggatattta cggttggtga
      661 tgaatgccat gctcattttc ttgttgaaca aggaaaggaa gctaaaatag cgctagagca
      721 agctggactg ttagtacttg cgatccggac accattaatt cgtaagttaa aacatgaaaa
      781 accgggggaa cttggcgaaa tagcacgagt attggcggag aataacatta atattttagt
      841 gcaatacagt gaccatgcta accaactgat attaataacg gacaatgata gtatggctgc
      901 atctgttacg ctcccttggg caataaagtg aacttgcgat ggctaattta atacgaaaag
      961 aggttacctt tgagtcctca atagccgcga taggggcggc tatgtctgac atttcacgag
     1021 ttaaaatact cagtgctttg atggatgggc gagcttggac ggccactgag ctaagttctg
     1081 tggcgaatat atcagcttca acggcgagca gtcatttatc taaattatta gattgccagc
     1141 taatcacagt agtagctcaa ggcaagcatc gttattttcg gctagcagga aaagatattg
     1201 ctgaattgat ggaaagtatg atggggatct ccttaaacca tggcgtacat gccaaagttt
     1261 ccacgccagt gcatttacga aaagcacgta cttgctatga tcatttagct ggcgaagttg
     1321 ccgttaagat ctatgattcc ctttgtcaac agcaatggat cactgaaaat ggttcaatga
     1381 tcacattaag tggtattcaa tattttcatg aaatgggaat tgacgttcct tccaaacatt
     1441 cacgtaaaat ctgttgtgcg tgtttagatt ggagtgaacg ccgtttccat ttaggtgggt
     1501 acgttggagc cgcattattt tcgctttatg aatctaaagg gtggttaact cgacatcttg
     1561 gttaccgtga agttaccatc acggaaaaag gttatgctgc ttttaagacc cactttcaca
     1621 tttaagttgt ttttctaatc cgcatatgat caattcaagg ccgaataaga aggctggctc
     1681 tgcaccttgg tgatcaaata attcgatagc ttgtcgtaat aatggcggca tactatcagt
     1741 agtaggtgtt tccctttctt ctttagcgac ttgatgctct tgatcttcca atacgcaacc
     1801 taaagtaaaa tgccccacag cgctgagtgc atataatgca ttctctagtg aaaaaccttg
     1861 ttggcataaa aaggctaatt gattttcgag agtttcatac tgtttttctg taggccgtgt
     1921 acctaaatgt acttttgctc catcgcgatg acttagtaaa gcacatctaa aacttttagc
     1981 gttattacgt aaaaaatctt gccagctttc cccttctaaa gggcaaaagt gagtatggtg
     2041 cctatctaac atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca
     2101 atacaatgta ggctgctcta cacctagctt ctgggcgagt ttacgggttg ttaaaccttc
     2161 gattccgacc tcattaagca gctctaatgc gctgttaatc actttacttt tatctaatct
     2221 agacatcatt aat
//
LOCUS       DAZFWL020000314         2219 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFWL020000314 DAZFWL020000000
VERSION     DAZFWL020000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.318"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            23..295
                     /locus_tag="ACHKNP_RS25990"
                     /old_locus_tag="pgaptmp_005198"
     CDS             23..295
                     /locus_tag="ACHKNP_RS25990"
                     /old_locus_tag="pgaptmp_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4707151.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            372..587
                     /locus_tag="ACHKNP_RS25995"
                     /old_locus_tag="pgaptmp_005199"
     CDS             372..587
                     /locus_tag="ACHKNP_RS25995"
                     /old_locus_tag="pgaptmp_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4707152.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGK"
     gene            592..936
                     /locus_tag="ACHKNP_RS26000"
                     /old_locus_tag="pgaptmp_005200"
     CDS             592..936
                     /locus_tag="ACHKNP_RS26000"
                     /old_locus_tag="pgaptmp_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HGU4707153.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVALEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPIILETQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            987..1520
                     /locus_tag="ACHKNP_RS26005"
                     /old_locus_tag="pgaptmp_005201"
     CDS             987..1520
                     /locus_tag="ACHKNP_RS26005"
                     /old_locus_tag="pgaptmp_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4707154.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1680..1817
                     /locus_tag="ACHKNP_RS26010"
                     /old_locus_tag="pgaptmp_005202"
     CDS             1680..1817
                     /locus_tag="ACHKNP_RS26010"
                     /old_locus_tag="pgaptmp_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707155.1"
                     /translation="MFDFSKLIREIRMMAEKLSTWKFILIWLVFVIMASGYFIGQIRW
                     W"
     gene            1819..2157
                     /locus_tag="ACHKNP_RS26015"
                     /old_locus_tag="pgaptmp_005203"
                     /pseudo
     CDS             1819..2157
                     /locus_tag="ACHKNP_RS26015"
                     /old_locus_tag="pgaptmp_005203"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            2129..>2219
                     /locus_tag="ACHKNP_RS26020"
                     /old_locus_tag="pgaptmp_005204"
     CDS             2129..>2219
                     /locus_tag="ACHKNP_RS26020"
                     /old_locus_tag="pgaptmp_005204"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4707156.1"
                     /translation="MKPPAPPAWIMQPAPDWQTPLNGIISSSEN"
BASE COUNT          571 a          498 c          644 g          506 t
ORIGIN      
        1 catctttcga cgagaaaatc ccatgtcaga aattacatcc ctggtcactg ctgaagcagt
       61 gaaggaagtc ctgcgctctg aagaagtccg gagcgcactg aaacaaaaac ttcgccataa
      121 cctggaagcg cgtcttgatg cagaggttga tgccattctg gatgagctgc ttggtgtaca
      181 ggcagagcca ccgactgaag cgggagatac caccgcagag agcggtgaag ttcagcctga
      241 atcaccggtc gccgatgcga ctgaacctca acccgaatcg gtcatgatgc tgtaacgggg
      301 agtcagggcc atcagtagtt aactggtggc ttttttattg ttgtcagctt ccggataacg
      361 ggagacgggg tatgtaccag atggaaaaaa tcacaacggg tgtgtcatac accacgtcag
      421 cggtggggac gggatactgg ttactgcagc tgctggacaa agtctctccg tcccagtggg
      481 tggcaatcgg tgtgctgggg agtctgctgt ttggcctgct gacgtatctt actaacctgt
      541 atttcaaaat cagagaggac cgtcgtaagg cggcgcgggg aaagtaaagc gatgaagaaa
      601 aaatacgaac tggttgttaa agggataaat aattacccgg ataagattac tgttactgtg
      661 gcactggaaa ttggtgggta tccgtcactg ttgttgccag atgtggcgat tagtcttgac
      721 cgtactgaag gtgccacgct ggagttttac gaagctgagg cgaaaaagca ggcgaagcag
      781 tttttcatgg atgttgctgc cgggttatgt gaaggggatg gtccgttgcc ggaaaagcgg
      841 cccatcattt tagagacgca ggatgtgttg ataacctaca gaggaaaact accgggaata
      901 attactggtt ctctgaagac tccaccgctg gcctgaagac ttaatatatc cagggatttg
      961 aaatcgataa accctgataa atatccatga acgcaaaaat cagatacggc ctgtcggctg
     1021 ccgttctggc gctgattggt gcaggggcgt ctgcgcctga aatcctcgac cagtttcttg
     1081 acgaaaaaga aggtaaccac accacggcat accgtgatgg tgcggggatc tggaccatct
     1141 gccgcggtgc catcctggtg gatggtaaac ctgtcgtccc gggcatgaag ttgtcgaagg
     1201 aaaaatgcga ccaggttaac gccattgaac gtgataaggc gctggcatgg gtggagaaaa
     1261 acatcagagt gccactgacc gaaccccaga aagcggggat tgcgtcattc tgtccgtaca
     1321 acattggccc cggtaagtgt ttcccgtcga cgttttacag acggattaat gccggtgacc
     1381 gcaggggagc atgcgaggcg attcgctggt ggattaagga cggtggcaga gactgccgta
     1441 ttcgctcaaa caactgctac ggtcaggtct cacggcgtga ccaggagagc gcgctggcgt
     1501 gctggggaat tgacagataa gcagaatatt ttgctgaaaa atgcgatttg ctcacgcgga
     1561 cggataacac gaaatcctgc gaactgacaa aaactaagtg aataaaagta aaaaccccgt
     1621 ttgttggctg caagcggggt tttgtgtttc ctgactccgg aaaagtcaaa ggagaaagtg
     1681 tgtttgattt tagcaaactg attcgggaga ttcgaatgat ggctgaaaaa ttatccacct
     1741 ggaagttcat ccttatctgg ctggtgtttg tgattatggc ttccggttat ttcattggtc
     1801 agatacgctg gtggtgaaat gaaccgcgta ctgtgcgtgg tcatcattgc cctgctggtg
     1861 gcctgtggtg cgcttagtct ggggctgaat cattaccgtg ataacgccat aacctacaaa
     1921 gagcagcgcg ataaaaaagt cagtgagctg gagctggcaa atgcaaccat tactgatatg
     1981 cagcagcgcc agcgtgatgt tgctgcactt gatgccagat actcgaggga attagccgat
     2041 gcgagagctg aaaatgaaac tctgcgcgct gatgttgccg ctggtcgtaa gcgcctgcgg
     2101 atcaacgcca cctgccccgg taccgtgcgt gaagccaccg gcacctccgg cgtggataat
     2161 gcaaccggcc cccgactggc agacaccgct gaacgggatt atttcatcct cagagaacg
//
LOCUS       DAZFWL020000315         2211 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFWL020000315 DAZFWL020000000
VERSION     DAZFWL020000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.319"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            74..406
                     /gene="hdeA"
                     /locus_tag="ACHKNP_RS26025"
                     /old_locus_tag="pgaptmp_005205"
     CDS             74..406
                     /gene="hdeA"
                     /locus_tag="ACHKNP_RS26025"
                     /old_locus_tag="pgaptmp_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HGU4707157.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VEESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            522..848
                     /gene="hdeB"
                     /locus_tag="ACHKNP_RS26030"
                     /old_locus_tag="pgaptmp_005206"
     CDS             522..848
                     /gene="hdeB"
                     /locus_tag="ACHKNP_RS26030"
                     /old_locus_tag="pgaptmp_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HGU4707158.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            912..1559
                     /locus_tag="ACHKNP_RS26035"
                     /old_locus_tag="pgaptmp_005207"
     CDS             912..1559
                     /locus_tag="ACHKNP_RS26035"
                     /old_locus_tag="pgaptmp_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGU4707159.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGIAGVKGVSIS"
     gene            complement(1601..2131)
                     /locus_tag="ACHKNP_RS26040"
                     /old_locus_tag="pgaptmp_005208"
     CDS             complement(1601..2131)
                     /locus_tag="ACHKNP_RS26040"
                     /old_locus_tag="pgaptmp_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HGU4707160.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
BASE COUNT          662 a          425 c          497 g          627 t
ORIGIN      
        1 aaaaccaggt tataacctca gtgtcaaaat tgattcgtga cggctctttc actttatagt
       61 tgaggatatt acgatgaaaa aagtattagg cgttattctt ggtggtctgc ttcttctgcc
      121 agttgtgagc aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac cggtcaactc
      181 ctggacctgt gaagatttcc tggctgtgga ggaatccttc cagccaactg cagttggttt
      241 tgctgaagcg ctgaacaaca aagataaacc agaagatgcg gttttagatg ttcagggtat
      301 tgcaaccgta accccagcta tcgttcaggc ttgtactcag gataaacaag ccaactttaa
      361 agataaagtt aaaggcgaat gggacaaaat taagaaagat atgtaattcc gggaatgcgt
      421 tacatcgtac ttccttgcat attgaacagg ccggaatatc ttctttaaaa gcagctattc
      481 ctcctgttca tatataatct ctatattgaa tgggttacaa aatgaatatt tcatctctcc
      541 gtaaagcgtt tatttttatg ggcgctgtag cggctttgtc actggtgaac gcacaatctg
      601 cgctggcagc caatgaatcc gctaaagata tgacctgcca ggaatttatt gatctgaatc
      661 caaaagcaat gaccccggtt gcatggtgga tgctgcatga agaaacagta tataaaggtg
      721 gcgataccgt tactttaaat gaaaccgatc tcactcaaat tcctaaagtg atcgaatact
      781 gtaagaaaaa cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct aatgacttgc
      841 cgaattaatg aggtacaagt aaaaaggagt agcaagttga gccatcttgc tgctcctttt
      901 tgcattttta tatgacagca gaatttatta tacgtcttat actcgcggca attgcctgtg
      961 gcgctattgg catggaaagg caaatgcgcg gcaaaggagc agggttacgc acacatgtat
     1021 taattggcat gggaagcgcc ctgtttatga ttgtttcgaa atatggtttt gctgacgtgc
     1081 tgtctttaga tcacgtcgga ctcgacccca gccgtattgc tgctcaggtg gtgacgggcg
     1141 tcgggtttat cggtgcaggt aacattctcg ttcgtaacca aaatattgta ggtctgacga
     1201 cggcagcgga tatctgggtg accgccgcca taggtatggt tattggcagc ggtatgtacg
     1261 aactgggtat ttatggttca gtgatgacct tgttggtgct ggaagtcttc catcaattaa
     1321 ccttccgcct gatgaacaaa aattaccatt tacagctgac attagtgaac ggtaatacgg
     1381 tatcaatgct cgactggttt aagcagcaaa aaataaaaac ggatttagtt tcgttgcagg
     1441 aaaatgaaga tcatgaagtg gtcgccattg atatccagct tcatgccaca acgtcgatag
     1501 aagacctgtt gcgattatta aaaggtatag caggggtgaa aggcgtttca attagctaat
     1561 ctgtgtggca gcgtagccag actcaccgta agcctgaaat tcacaccaga taatcaatat
     1621 gctgatggcg taccagctca ttgatccgct taagattgag gatcatcatg atattgtgtt
     1681 tatggcagta gaacgttttt agcgatattt tcagtttctc cagaatatct tcagttgtca
     1741 tttgattcgc aatgcagctc agaacctgat gttgcttgcg cgaaagtgac acattcgctt
     1801 caggcacaga gcagtaactg ccattgatga gtgcgacaaa atcaatgata gtgatagtcc
     1861 gcggaacaaa ggtcaccggt actttcccca gacagcgttt aatattaaag ggggagaaaa
     1921 caatgacatg gacaggtttt aatcgttcaa tttgagtgag aaaattagaa tgaaatacat
     1981 tattcattaa tgtatcaata atgacgaagg cattctgatg caacattacg tcgatctctt
     2041 cttcgttctg tatatgaacg acattacctt tactcagaat gtttttcatc gcggtgaaga
     2101 acatcgtatc cctggtaatt ataagaaaca taaccgactc ctggtgcgga cattaacttt
     2161 ataatcagtt tgccaaaggt aattaacagg acagggctta taataataat a
//
LOCUS       DAZFWL020000316         2203 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFWL020000316 DAZFWL020000000
VERSION     DAZFWL020000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.320"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1324)
                     /locus_tag="ACHKNP_RS26045"
                     /old_locus_tag="pgaptmp_005209"
     CDS             complement(<1..1324)
                     /locus_tag="ACHKNP_RS26045"
                     /old_locus_tag="pgaptmp_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HGU4707161.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKIT"
     gene            complement(1413..2201)
                     /locus_tag="ACHKNP_RS26050"
                     /old_locus_tag="pgaptmp_005210"
     CDS             complement(1413..2201)
                     /locus_tag="ACHKNP_RS26050"
                     /old_locus_tag="pgaptmp_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4707162.1"
                     /translation="MINFGFTRTDAQVYISLLKNGRSSGYKIAKEISLSRSSVYSSID
                     NLYKNGYIFLVDGETKEYEAKSPELILSQIEKSTIDNIKILKKELSQMMFQNEREFIY
                     NVSGFDNLHQKAKEMLSSAECEIYLNTDFDLHFLKNEICEALNRHVRVIVFSFNKLIT
                     PDPRVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSNNRLLINIIAEH
                     IHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
BASE COUNT          696 a          417 c          386 g          704 t
ORIGIN      
        1 gtgtaatttt aattgcaaca ttattaaatg tgccataaaa ctcacacggg ccataaaata
       61 atgcagtaaa accattgtta ttttcttgaa acatattttc gacaaaagcc ggtatggttc
      121 ttgctgtatt cggtacgcag cagacagcag cctcctcatg agtaggggag tatttatatc
      181 tggtattaaa ctgattactg tccggatgtt tcctttcatt tgcttcataa caattatcag
      241 tcttacagta cattatcgcg tgaagtatgg gatgctgcat gccaaaacta gcattataga
      301 atagccactc agcatcatcg gccagaccaa tattagtggt cagttttatt aacataacat
      361 aagtgctaaa tagttcatga acagaacaat attcatagcc tgtataagtg gcattagctg
      421 tgcgtcctgt aatccactca tcaccaatgg gagcgccaga tggtgtaaga taatatttca
      481 actgggataa aaaaatatct aatgcagttt gatactgcgg ccgttggctc gctgcgatgg
      541 ctaatgccct aatatgctca tacgtatgta cgccatgacc tttaagaagg taaccagggt
      601 ctatgacatt tttatattgc agatcttgct ccgacacgca tcctgcggaa taatcttcat
      661 acagccacaa gcaatatgag agatattcaa tgttattggt tatttcataa aggcggtaga
      721 aaacatctgt tattgttaat ccgtggctat gtccagcaaa accctgttcc acaacgaaag
      781 gatgtgacgt ttctttaggg tagccagcca taatgcattg tgcggcttgg gtaagagaag
      841 ttaataaact ctcattacgg gtggactcat aacatccaag cacaaaacgc aaggcagtag
      901 cttgtgccca gaactctcca ttttctgctt tacagttaaa ttttaatgat gaatcataaa
      961 ttccaatata gccatcatca tcttgcgtat cgataagttc caaacataaa tcgctcgctt
     1021 tttcctgcca ttctttatta tctaacaagt aggcactgcg gcaatatcca tccttccaat
     1081 tcgattgagt ttcactatcc caccaataat attgcatcat gttatcatcg ggattattct
     1141 gttttttaat aacgccatct tcagcagata cagtgcgtgg agttatacga tctcttccat
     1201 atattttatg ctccgtaata agatggggaa ctaatttatc cagttctcct atatgaccgt
     1261 tcatatacca ttccatctta ttctttaacc aaccagtggg ctttatatcg ttaaaaaaaa
     1321 tcataaatat acctttgcat aaaaaagttt agacgttata aaagacaaat agattgtgat
     1381 cgcctacaga cgatcacaat gaaaggaaaa tattaacggt ttttgctatc actcaaatgt
     1441 ttttcatcct gcaccatatc attgccagat tcatgcaaag tatttattaa tgcattggca
     1501 tctggatatt gttgctgata cttggttaga tatatatcac tatggatatg ttcggcaatt
     1561 atattgataa gcaagcgatt attcgagtat aaccccagtg attcagctcg ttgtgaaaaa
     1621 accattactg tcttcatatc aacaacaagc ataaaacggt gagaagggta atattcttct
     1681 tcatggttaa agcggtaata cagttctaca cgtgggtcgg gagtaataag tttattaaat
     1741 gaaaaaacaa taactcgaac atgtcgattc agagcctcac aaatttcatt tttcaaaaaa
     1801 tgtaagtcaa aatccgtgtt gaggtaaatt tcacactctg cagaagaaag catctcttta
     1861 gctttctgat gtaagttatc gaaaccagag acattataaa tgaattcacg ttcattctga
     1921 aacatcatct gtgataattc tttttttaga atttttatat tgtcaatagt gcttttttca
     1981 atttgtgaaa gtatgagttc aggagatttt gcttcatatt ctttagtttc accatcgaca
     2041 agaaagatat aaccgttctt gtaaagatta tcaatagatg agtatacaga cgaacgagaa
     2101 agtgaaattt cttttgctat cttgtaacca ctgctacgac cgtttttcaa cagactgatg
     2161 taaacctgtg catcggtgcg agtgaaacca aaatttatca att
//
LOCUS       DAZFWL020000317         2184 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFWL020000317 DAZFWL020000000
VERSION     DAZFWL020000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.321"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(402..584)
                     /locus_tag="ACHKNP_RS26055"
                     /old_locus_tag="pgaptmp_005211"
     CDS             complement(402..584)
                     /locus_tag="ACHKNP_RS26055"
                     /old_locus_tag="pgaptmp_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU4707163.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRYRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            complement(581..1108)
                     /locus_tag="ACHKNP_RS26060"
                     /old_locus_tag="pgaptmp_005212"
     CDS             complement(581..1108)
                     /locus_tag="ACHKNP_RS26060"
                     /old_locus_tag="pgaptmp_005212"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU4707164.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            complement(1105..1551)
                     /locus_tag="ACHKNP_RS26065"
                     /old_locus_tag="pgaptmp_005213"
     CDS             complement(1105..1551)
                     /locus_tag="ACHKNP_RS26065"
                     /old_locus_tag="pgaptmp_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGU4707165.1"
                     /translation="MKQIYMLRNEAIRNNAIDAILSLPIDDKSPHEVHIKEPKRSNPQ
                     NRLMWALLQDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            complement(1559..1765)
                     /locus_tag="ACHKNP_RS26070"
                     /old_locus_tag="pgaptmp_005214"
     CDS             complement(1559..1765)
                     /locus_tag="ACHKNP_RS26070"
                     /old_locus_tag="pgaptmp_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707166.1"
                     /translation="MVELIFSALRILGAMWMVATFIVVASSFVRLVGEGKDLVGVLFG
                     SIFLWVIIGVAPVAVAKMAWRFVS"
     gene            complement(1838..2128)
                     /locus_tag="ACHKNP_RS26075"
                     /old_locus_tag="pgaptmp_005215"
     CDS             complement(1838..2128)
                     /locus_tag="ACHKNP_RS26075"
                     /old_locus_tag="pgaptmp_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HGU4707167.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATKEEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
BASE COUNT          543 a          606 c          435 g          600 t
ORIGIN      
        1 tttacttaat gtatttctat aaaagcaaat tacaaactca caacaaaaaa cctcataaca
       61 cattaacaat caattctttt catctttata aattctcata tcaggcttgc acccgataaa
      121 ccgacgaaaa ctatttaaaa cccatcgagt gaagtaatct ctaaaaccaa agaaatacca
      181 agtgaaaata ttcacgataa aatgcccggt caaagcccct cctgtaccgc atgcaagaac
      241 agtaaaaaat cagatgtttt cataaatatc agtcctcatc gttttgcctg gcatgtcctt
      301 taccagcaat cttctgtatg cactaagcct agatagaatc cactcagtgt acactgaagc
      361 ccgctcgacg ctttcttgtt cgtaacttcg attttagtca attaccttgt tttcctcgca
      421 cgatgtctta gccaccggat atcccacagg tgagccgtgt aattgaaggt ttttacgtca
      481 gattcttttg ggattggctt gcgtttattt ctgtagcgtt tcgttggaag gtatttgcag
      541 ttttcgcaga tgatgtcggt gaaacttcgt cgctgtcgcc tcatgccgcc ctcctgacgc
      601 cctgcccgat cgccatcaat gccgctttgg atacggtagt aaacatccgt cgaggactga
      661 tgaacggtcg ccaaatcagc agcatggagc ctttactgtt tcccttcttc tccagccctg
      721 tcgatggttc gataaaatta atccgtccat cagtgataat gcgaacttcg tcaacactct
      781 ccagagcctt gctgaaccat ccgacagaca tatcctctgg cacaagcatc actaccgtct
      841 gtcgctgttg tatgcactgc tcagcggctt tttccaccca cggcctgata ttgctgtacg
      901 gtgggttatt ccagattgca ccgtggctta tccactcaga attgagtgcg tcgtcggcct
      961 cagttagcca gtgagcgcac agagcatttt tgtcgctcgc agctgaatcc agccagaatc
     1021 caaactcaat atccagcgca tcaaaaagcc aaagcggcgt ttgccagcag tccttgtcgt
     1081 gtgctggtgt atttgatttg atagtcatgc agccctacct tttcgttgtg accattcata
     1141 ctctcgccgg gagtcatcac tccaccgcac gttgcgctct gagccgaacc aaaacatgat
     1201 ttcgataagc tcagtcatgc tggcctttcg cattttgctg gtacgcacgc caagcatgac
     1261 aacgccacca tcgataccag gcacacttcg ttgctccagt tttttggtct taagccacag
     1321 ggcagtgaac aggtctttcc agtcctccgg cgcaagtctc tgtccatgcc aaagcacctg
     1381 acgtgatacg tcctgcaata acgcccacat aaggcggttt tgaggattgc tccgctttgg
     1441 ttctttaatg tggacttcgt gaggtgactt gtcgtcgatc ggaagtgaga gtattgcgtc
     1501 tatggcgttg tttctgattg cttcgttgcg aagcatgtat atttgcttca ttgtcacctc
     1561 aactcacaaa acgccacgcc atttttgcta cagcgacagg cgcaacaccg ataatcaccc
     1621 acaggaaaat gctaccgaaa agcacaccaa ccaggtcttt accttcgcct accagccgga
     1681 caaaactgct ggcaaccaca atgaacgtcg ccaccatcca catagcaccg agaatcctca
     1741 atgcagaaaa aatcaactca accacgattt actctccccc aaataaaaag gcctgcgatt
     1801 accagcaggc ctgttattag ctcagtgatg tagatggtca tcttttaact ccatataccg
     1861 ccaatacccg tttcatcgcg gcactctggc gacactcctt aaaaattagg ttcgtgctca
     1921 tctttccttc ccgttcttcc ttggtagcaa accggtaata caccgttcgc cagaccttac
     1981 cttcgataac cagaagacct gcccgtgcca ttttagccgc ggcctgattt atgctggtta
     2041 ctgttgcgcc tgttagcgcg gcaacgtccg gcgcacagaa gctattatgc gtccccaggt
     2101 aatgaataat tgcctctttg cccgtcatac acttgctcct ttcagtccga acttagcttt
     2161 gagttctgcg atcttcgcca gagc
//
LOCUS       DAZFWL020000318         2154 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFWL020000318 DAZFWL020000000
VERSION     DAZFWL020000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.322"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..275
                     /locus_tag="ACHKNP_RS26080"
                     /old_locus_tag="pgaptmp_005216"
     CDS             6..275
                     /locus_tag="ACHKNP_RS26080"
                     /old_locus_tag="pgaptmp_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4707168.1"
                     /translation="MNILKKLMQRMCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRGDGTWENIGGMK"
     gene            complement(392..667)
                     /locus_tag="ACHKNP_RS26085"
                     /old_locus_tag="pgaptmp_005217"
     CDS             complement(392..667)
                     /locus_tag="ACHKNP_RS26085"
                     /old_locus_tag="pgaptmp_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HGU4707169.1"
                     /translation="MPFSIKNIFSNSKGSYPEISGPVQDKPVSKNCTLTSTTCSMNDY
                     TVFSRKSCTFDMRPPGAGDRTPQLKLSASELIWLSKTIDTERNNIKE"
     gene            complement(730..2091)
                     /gene="espK"
                     /locus_tag="ACHKNP_RS26090"
                     /old_locus_tag="pgaptmp_005218"
     CDS             complement(730..2091)
                     /gene="espK"
                     /locus_tag="ACHKNP_RS26090"
                     /old_locus_tag="pgaptmp_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HGU4707170.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSAGIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTILTGLPVNTQNLSVINCEKLQITDIPSTVKN
                     LHIELTDSPFIHIIPEGIECLTVCRCHISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLQTLSLTGCSNIILPEILPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKSH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADEIVNSLKLIANKSDG
                     YGQSITASELRWIYRNKDNDQIMKNIKFYLRGKEIPAEKILGAPEWKDYKPKYSGATY
                     KYS"
BASE COUNT          511 a          407 c          507 g          729 t
ORIGIN      
        1 atgacatgaa catactaaaa aaacttatgc agcgtatgtg tggttgcgga aagcatgatg
       61 gccgtgaaca cgtgcagtcg cttacagcac agctgcgact gggaccggca gacattctgg
      121 agtccgatga gaatggcatt atcccggagc aggacagggt aatcacgcag gtggtgatac
      181 tggatgcgga taaaaagcag atacagtgcg tggtaagacc gctgcaaatt ctgcgtggtg
      241 acgggacgtg ggaaaatatt ggcgggatga agtaactcga cagcttcaca aaaccggagt
      301 ccggctccat tttaccacct ccacgggagg tggttgcttg caatcagatg tttcgggcct
      361 gcggcttttt gtgttcccta gtaagtggtt actattcttt tatgttattc ctctctgtat
      421 ctattgtctt agatagccat atgagttcag atgctgagag ttttaattgt ggtgttctgt
      481 ctcctgctcc gggtggacgc atgtcaaaag tgcaggattt tctgctgaat actgtgtagt
      541 cattcataga gcatgttgtt gaggttagtg tacaattctt cgatactggc ttgtcctgca
      601 caggtccgga tatttctggg tagcttcctt ttgaatttga aaatatgttt ttgattgaaa
      661 atggcatcgt gctttgtcct ttgaactacg atatattgtt ttgtgttctt tcgttgtgag
      721 taggtatcat taagaatact tatatgtggc tccagagtat ttgggtttgt agtctttcca
      781 ctctggtgcg cctaatattt tttctgctgg tatctctttc ccacgcaagt aaaactttat
      841 gtttttcatt atctggtcgt tgtctttgtt gcgataaatc catcgtaatt cgctggcagt
      901 aatactctga ccataaccat ctgacttatt agcaatgagt ttgagtgagt tgacaatctc
      961 gtcagcacaa aaaataaccg ttttgttgcg tgttaatgtc tgaaactcca ggccggcctt
     1021 gcttgttttt gcccataatt tgtcgcgatt gtatacggaa aggtcggcca tggtgacatt
     1081 ataattattg tggctcttta ggtgttgttt aaattttatc cctctttcgt gttcatttaa
     1141 tgctcttttg atcagtggat cttgcttata tttgcgaggt gttctggggt cccatactgc
     1201 gtttgttaac gtattctgag ttataatatc ttttttggaa aagtcgttaa ctagtttaat
     1261 gctgttgaca agtttttctc tgggtgaaga tagtccatag acaatgtctc cctcgcgaaa
     1321 gtgtaaggcc acatccagag cgttgccctg aaaggtgatg ttttttgctt taactacatc
     1381 tggagagagt agtacatttt gtaaatcaag atccagccca tcaggcatcc cttcaacacc
     1441 gatgttccac gtggttttct gctccgcatg aatggttacc gatgtcacac tctccggaag
     1501 tatctccggc agtataatat tgctacatcc agtcagcgat agcgtctgca gtgacggtga
     1561 tatcaggttg tcaattctgg cctgattctc cgggttatag ctattgatgc tgagagatga
     1621 taacccgttt ggaacatttt ttatgctgtc tgtggcgcta ccttttatct caaggtagcg
     1681 gacgctctct ggcactccag atatatggca gcgacaaacc gtcaggcatt cgatgccttc
     1741 aggtatgatg tgtataaatg gtgaatcagt taattcaata tgtagatttt ttactgtcga
     1801 tggtatgtct gtgatttgta atttttcaca gttaatgacg gagaggtttt gtgtattaac
     1861 cggcaatcct gtaaggattg tcaggttgtt gcagttttcg attgtaactg atgttatttc
     1921 gtttggtaac tgtggcagat actcaatatc acaatctttg atatataaac gaccactggc
     1981 ccgcgcttct tctatctgaa ttggtgtgat ttcgcttttg atgcctgctg atgtttcagc
     2041 ggaataggag cagaatgtcc cggtcggtcg taattgcgat gtaggaagca ttgataaatt
     2101 ccatttttag aagtaaataa cagtgtaagt tctatccata ctgctaatca acaa
//
LOCUS       DAZFWL020000319         2121 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFWL020000319 DAZFWL020000000
VERSION     DAZFWL020000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.323"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1709
                     /locus_tag="ACHKNP_RS26095"
                     /old_locus_tag="pgaptmp_005219"
     CDS             <1..1709
                     /locus_tag="ACHKNP_RS26095"
                     /old_locus_tag="pgaptmp_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HGU4707171.1"
                     /translation="SGCGPESENKESQQQQPSTPTDQQVLAAQQAAIKEAEQRSVADK
                     ATADAKAKALAQQEAQQYSDKQALQGRLQAAPKYQHAAREKAASQIANPGTARYKQFD
                     DNPVKQVAQNPLATFSLDVDTGSYANVRRFLNHGLLPPPDAVRVEEIVNYFPYDWDIK
                     DKQSIPATKPIPFAMRYELAPAPWNEQLTLLKIDILAKDHKSEELPASNLVFLIDTSG
                     SMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAEINAAIDS
                     LDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSIESMVKKQR
                     ESGVTLSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQTLITVAK
                     DVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHITLLFELTLNGQ
                     KASIDKLRYAPDNKSVKSDKTKELAWLKIRWKYPQGKESQLVEFPLGPTINAPSEDMR
                     FCAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYRAEFIRLIELADGVTDI
                     SQ"
     gene            complement(1780..>2121)
                     /locus_tag="ACHKNP_RS26100"
                     /old_locus_tag="pgaptmp_005220"
     CDS             complement(1780..>2121)
                     /locus_tag="ACHKNP_RS26100"
                     /old_locus_tag="pgaptmp_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HGU4707172.1"
                     /translation="VHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESNSDS
                     APYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIKHSQ
                     PKNRASGKRVS"
BASE COUNT          611 a          464 c          514 g          532 t
ORIGIN      
        1 tgtcaggatg cgggccggaa tctgagaata aggaaagtca gcaacaacag cccagtaccc
       61 ccacagacca gcaagtgctt gccgcgcaac aagctgcaat aaaagaggcc gagcaaagat
      121 ccgtcgccga taaagccact gcagacgcga aagcaaaagc cttagcccag caagaagcgc
      181 aacaatattc agacaaacag gctttgcagg ggcgcttaca ggcggctcca aaatatcaac
      241 atgcagccag agaaaaagca gcgtcccaaa tcgcaaatcc aggaaccgct cgctacaagc
      301 agttcgatga taatccggtt aagcaggtag cgcaaaaccc gttggcgacg tttagccttg
      361 acgtcgacac cggcagttat gcgaatgtac gccgttttct caatcatggg ctgttacctc
      421 cgccagacgc tgtgcgggtg gaggagatag tcaattattt cccgtatgac tgggatatca
      481 aagacaaaca atctattccg gccactaagc caataccttt cgctatgcgc tacgaactgg
      541 ctcctgcgcc gtggaatgaa cagctaacat tgctgaaaat tgatatcctg gcgaaagatc
      601 ataaaagtga agagttacca gcttctaatc tggtctttct tatcgacact tctggttcaa
      661 tgatttctga tgaacgtttg ccacttatcc agtcttcgtt gaaattattg gtcaaagaac
      721 ttcgtgagca ggataacatt gccatcgtga cctacgctgg cgactcccgt attgcgttgc
      781 cttctatctc cggtagtcac aaggcggaaa ttaatgccgc aattgattcg ctggatgccg
      841 aaggcagtac caatggcggt gccgggctgg aactggctta tcagcaggcg gcgaaggggt
      901 ttattaaggg cggcatcaat cgcattttat tagccactga cggtgacttt aacgttggca
      961 ttgacgatcc aaaatcgatt gaatcaatgg tcaaaaaaca gcgggagtct ggtgttactc
     1021 tgtcgacgtt tggcgtgggg aatagcaatt acaacgaggc aatgatggtg cgaattgccg
     1081 atgttggtaa cggcaactac agctacattg ataccctctc tgaagcgcag aaagtattga
     1141 atagtgaaat gcggcagacg ttgattaccg tagcaaaaga tgtcaaagcg caaattgagt
     1201 ttaaccccgc gtgggtaacg gaataccgtc agattggtta tgaaaagcgc caacttcggg
     1261 cggaagattt taataacgac aacgttgatg cgggggatat tggcgcaggc aaacatataa
     1321 cgttgttatt cgaattaacg ctgaacgggc aaaaagcatc aattgataag ttacgctatg
     1381 ccccggataa caaatcagtg aaatcggaca aaacgaaaga actggcctgg ttaaagattc
     1441 gctggaaata cccgcaggga aaagaaagtc agttagttga attcccgctg gggccaacaa
     1501 taaacgcccc ctctgaagat atgcgttttt gcgcagcagt agctgcatat gggcaaaagt
     1561 tacgcggttc tgaatacctg aacaatacct cctggcagca gatcaaacag tgggctcagc
     1621 aggcaaaagg ggaagatcca cagggttaca gggcggaatt tattcgcctg attgaactgg
     1681 cggatggtgt gactgacatc agtcagtgat gactgtttag caaactatgt tcgaccagtc
     1741 agcatatttg ctgactggtc gaattgatta acaatgatgt taactcactc ttttgccgga
     1801 tgctctattt ttgggttgtg agtgttttat ttctaattgt gtataagaag cattgattcg
     1861 tgcatcgcca tatttatcta aataacaatt tctgtttacc agtcgatgtt gaaaatcaat
     1921 ttcgtactgc tcttctgctg aatatttttt aaatctgtca gcatagtttt ttaataacgt
     1981 atagggggcg ctgtcagaat tgctttccct ttgttcaatc acttcctggg ctgccatgca
     2041 cacaaacgca ccgcaacttt ggcttaagta ttgctgaaga tcggcatgta aaaatattat
     2101 ctctggttca tttaaatgaa c
//
LOCUS       DAZFWL020000320         2115 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFWL020000320 DAZFWL020000000
VERSION     DAZFWL020000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.324"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            35..523
                     /locus_tag="ACHKNP_RS26105"
                     /old_locus_tag="pgaptmp_005221"
     CDS             35..523
                     /locus_tag="ACHKNP_RS26105"
                     /old_locus_tag="pgaptmp_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HGU4707173.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLIYSEKAGAVTMQFATMN
                     WNPQFTVTGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIAAMVAP
                     CK"
     gene            606..1733
                     /locus_tag="ACHKNP_RS26110"
                     /old_locus_tag="pgaptmp_005222"
     CDS             606..1733
                     /locus_tag="ACHKNP_RS26110"
                     /old_locus_tag="pgaptmp_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4707174.1"
                     /translation="MAAVILRKVEKIYDNGFKAVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMAFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRQRVAVGRAIVRKPDVFLFDE
                     PLSNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRI
                     MQVDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAKNIRLYIPENKQ
                     SVLKAYIDKPVCFGIRPEHISLSLNCKEMNTVTGALSIIENMGSEKYLYFTVNNKEFI
                     AKVNDQSITTADIGKNLYFNLYTSFCHIFDFYTEENLTNYL"
BASE COUNT          669 a          383 c          428 g          635 t
ORIGIN      
        1 taactaagct atactacttt tattgagagt ttaaatgaaa aataaattgc ttattgcggc
       61 tgtgttgact ggtcttcttt caggatgcgt tgatggcaat tcaccctctg gcagtcagga
      121 aggtgtcgtg aaagcggatc ctcgctattc agaatgtaac ctccctgatg taaatgaaag
      181 agggccaatt gctgcgggtc tttacatttc cggaaccctc ccggaaggct attggttaat
      241 gaagccagag cgtaaaatgc attataaggg agatggtatt tatcagctta tttattctga
      301 aaaagcaggt gcggtaacaa tgcaatttgc gactatgaac tggaacccgc agttcactgt
      361 aacaggtctt gaattgccat taaatcaggc taaaacactc aagaaaggag gcatggataa
      421 aaatacaaaa gtcgttattc ctgccgatgg aaagtatgtt tggacgattc aaattggtcc
      481 tgataaaaag gcaatagccg ccatggtagc accatgtaaa tagttatttt tagcactcca
      541 ctattcaaca ttcgttgaat agtggagtat cagtattcca aaattcttgt taatgagagc
      601 agattatggc cgcggtaatt ctacgcaaag ttgaaaaaat ttatgacaat ggattcaaag
      661 ctgttcacgg cattgatctc gatataaacg atggtgaatt catggttttt gttggaccat
      721 ctgggtgtgc caaatcaact acattacgca tgattgcagg tcttgaagac gtatcaggtg
      781 gtgaaattta cattagcgac cgccttgtta atacccttcc gcctaaagat cgcagcattg
      841 caatggtgtt ccagaactat gcgctgtatc cccataagac tgtatttgac aatatggctt
      901 ttggtttaaa aatgcaaaaa aggccaaaag aagagattaa gaaaattgtc gaagaagttg
      961 cagaaaaact tgaaattagc gatttacttt accgaaaacc gaaagaaatg tcgggtggtc
     1021 agcgtcagcg tgtcgcggta ggccgtgcaa ttgtccgtaa gccagatgtt ttcttatttg
     1081 atgagccact atctaacctt gatgcaaaac ttcgtgtttc aatgcgagtc aaaattgctc
     1141 agttgcatca gtctctcaag gatgagggtc atccagcaac catgatttat gttacacacg
     1201 atcaaattga agcattgacc ctcggtgatc gtatttgtgt gcttaatcag gggcgaataa
     1261 tgcaggtcga cacaccagtt aatctgtata accaccctgc taacagattc gttgctggct
     1321 ttattggttc cccgtcaatg aatttgatag atgcggttgt acgtgaggaa gacggtaaac
     1381 tctatattga cattgccaaa aatattcggt tgtacatacc tgagaataaa caatcagtac
     1441 tcaaagcgta tatagataaa cccgtttgtt ttggtattcg tccagaacat atctctttga
     1501 gtttgaattg caaagaaatg aatacagtca ctggtgcatt aagtattatt gaaaatatgg
     1561 gcagtgaaaa atatctttac tttaccgtta acaacaaaga gtttattgca aaagtcaacg
     1621 atcaatcaat tactacagcc gatattggta aaaatttgta tttcaacctt tatacaagct
     1681 tctgtcatat atttgatttt tacactgaag aaaatctaac taattatttg tgattgatat
     1741 gacaataacc ccaggttgaa taacggtatt aaaccttaca attccataag gcttcttact
     1801 gctcgaactg tctattggta aatgtcatta actccacgaa ctgattctta tcaaggtaat
     1861 tgagttacaa aattccatcc ataaaaaaga gcatatatga ataaaccgtt gataacaccg
     1921 cttactcagt gagcctacta tttaggtcag agcatgtctt ctgaccatag ttggagggca
     1981 ttcagattcg cagttcacaa tgcttacgta ctgtagacct gtaaccagct tgaggatgaa
     2041 ttacgataaa tccacatttt agctcagcaa ttttttcgaa aatgttaatt aataatgtta
     2101 aaattggtaa gattt
//
LOCUS       DAZFWL020000321         2112 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFWL020000321 DAZFWL020000000
VERSION     DAZFWL020000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.325"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..522)
                     /locus_tag="ACHKNP_RS26115"
                     /old_locus_tag="pgaptmp_005223"
     CDS             complement(<1..522)
                     /locus_tag="ACHKNP_RS26115"
                     /old_locus_tag="pgaptmp_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4707175.1"
                     /translation="MRKLYAAILSAAICLAVSGASAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDIRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVSYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAI"
     gene            complement(593..>2112)
                     /locus_tag="ACHKNP_RS26120"
                     /old_locus_tag="pgaptmp_005224"
     CDS             complement(593..>2112)
                     /locus_tag="ACHKNP_RS26120"
                     /old_locus_tag="pgaptmp_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4707176.1"
                     /translation="GEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTET
                     TYRFTQLALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPH
                     LAVYDPTVQFEFWFSETRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSV
                     NTVGKSAFVEGVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSK
                     EWKDANDKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPA
                     NGNETPMFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGRV
                     NANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTV
                     TISDDHNFDRQIIIPPIIFNGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETK
                     GAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT          466 a          622 c          495 g          529 t
ORIGIN      
        1 aatggccacg gattcggtcg ggttaaactg cacgccagct ccccacgcca gagacgtgtt
       61 gctgtggcga ccgtcatcac ttccggtcag cacgtcgtgc gttttcccct tgttgtcagt
      121 tacgcggaga taatccccgg agaaagtcga cacacggctg taagacacac ccgccatcgc
      181 atacgcgctg aaccattcat tcacgcgcac agacggcccc gccatcacgc tgaaccagcg
      241 gttacgcacg gaatcttcat gccagcggat atcgctgtaa cgggtaagct ggcgattctt
      301 gtctcctgca tagctgaatg acgtcaccag ccccagtgtg tccgtaaact cataacggta
      361 tttcacgtta atcccgttca gatcatcgct gccgggaacg ttcgtcgagg catgaagata
      421 ccccgcgctc agcgtggact gatgttcaga tgcccatgca gacgcaccgg atacggccag
      481 acaaatagct gcggacaaaa tggctgcata aagtttacgc ataattacct ctcgcttttc
      541 tgcaataaaa aaggcgtcat tcctgacgcc ctttattggg gttataaata tttcaacgaa
      601 tactgatacc ggaagccgct tttttggtca caatcaccgt acagtcggtg atattccctg
      661 ccccctgatt gcctttctgg aaaatcttaa actccagagt gacgctacca ccaccactag
      721 gcatatcaat aactgcactg taactaccgg gaatggcccc tttagtttct ctggatgcga
      781 ttaatacacc gtttttgcga acttcaaaac cataacccgt gtatcttgta cctcccgggt
      841 tattaccact tcccggatcg ctatacgcta ttccgttaaa gataatgggc ggaataatga
      901 tttgacggtc aaagttatga tcatcgctga tggtgactgt aaccgtcccg tttggtgttt
      961 ccgtattacc ccacgtacca gcctgtttcg ggaatgattt ggatacagct ttaacgaagt
     1021 cacctctgac ctgagtcgcc tccagcatgc ccttaatcgt acagttttca tttaccgtga
     1081 cattgttgag cgtcccggcg ttcgcattca ctctgccact gatatccgca tttttagcgg
     1141 tcagctttcc gtctgatgtc agggaaaata ccggaggact gccaccgctg gtaatggtgg
     1201 gggctgtcag gcgcttcagg aacacgtcgt tcatgaatat ctggttgccc tgcgccacaa
     1261 acatcggcgt ttcattcccg tttgccgggt caataaacgc gatacggtta gcggcaacca
     1321 gaaactggct cagcttgcct tcctccgtgt cctccatgct gaggccaata cccgcgacat
     1381 aatgtttgcc gtctttggtc tgctcaattt tgacgcccca catggcattc catttatcgt
     1441 tagcgtcctt ccactctttc gaaaactcat ccagtctgct ggcgttatcc tccgtcaaat
     1501 caactttttc cagcagctct ttaccgagat gggattcggt tatcttgcct ttgaaaaaat
     1561 ccaggtaacc ttccgcatca tcgctcgccc gaccgacgcc ctccacgaat gccgatttgc
     1621 caacggtgtt cacactgcgg atataaaagt aataatcatg gcccggtttg atattgatac
     1681 tggcggctat ccagtacagc gccgtaccaa gatagcgggc tgtggtttca acctgcctga
     1741 tatcggtaat ccgcgtttcc gagaaccaga actcaaactg taccgtcggg tcataaacgg
     1801 caagatgcgg cgtggcggtt atctgaaaat agcccggcgt cagctcaatc ctcgacggtg
     1861 ctgccggtgc ggcaatccgg aacgataccg acgccggatc gccctgctgc ccccacgcat
     1921 ttaccgcccg gactgtcagc ctgtagttcc ccagcgccag ttgcgtgaag cggtatgtgg
     1981 tttccgtcgt ccgggccgtg ctgaccagcc gctcactgcc gtcatccgct gttacggtca
     2041 gacggagcag gaagctcacg cccttcacca ccttcggcgt gtcccagcgc gccagcacct
     2101 gatattcccc gc
//
LOCUS       DAZFWL020000322         2108 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFWL020000322 DAZFWL020000000
VERSION     DAZFWL020000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.326"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            182..397
                     /locus_tag="ACHKNP_RS26125"
                     /old_locus_tag="pgaptmp_005225"
     CDS             182..397
                     /locus_tag="ACHKNP_RS26125"
                     /old_locus_tag="pgaptmp_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4707177.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKTARGD"
     gene            402..746
                     /locus_tag="ACHKNP_RS26130"
                     /old_locus_tag="pgaptmp_005226"
     CDS             402..746
                     /locus_tag="ACHKNP_RS26130"
                     /old_locus_tag="pgaptmp_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HGU4707178.1"
                     /translation="MKKKYELVVKEINNYPDKIAVTVALEIGGHPSLLLPHVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            797..1330
                     /locus_tag="ACHKNP_RS26135"
                     /old_locus_tag="pgaptmp_005227"
     CDS             797..1330
                     /locus_tag="ACHKNP_RS26135"
                     /old_locus_tag="pgaptmp_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4707179.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1601..>2108
                     /locus_tag="ACHKNP_RS26140"
                     /old_locus_tag="pgaptmp_005228"
     CDS             1601..>2108
                     /locus_tag="ACHKNP_RS26140"
                     /old_locus_tag="pgaptmp_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGU4707180.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEA"
BASE COUNT          577 a          447 c          583 g          501 t
ORIGIN      
        1 tgctggagat aaatgcaccg gcatcatcat aaaaagtgag ccagtcaaat ggaaggtatc
       61 gttaaactca ccggtagtgt cagtgggtcg tctgagacgc ctgcatgagt tatcagagcc
      121 atcagtagtt aactggtggc ttttttattg ttgtcagctt ccggataacg ggagacgggg
      181 tatgtaccag atggaaaaaa tcacaacagg tgtgtcatac accacgtcag cggtagggac
      241 gggatactgg ttactgcaac tgctggacaa agtctctccg tcccagtggg tggcaatagg
      301 tgtactgggg agtctgctgt ttggcctgct gacgtatctg actaacctgt atttcaaaat
      361 cagggaggac cgccgtaaga cggcgcgggg agactaaagc gatgaagaaa aaatacgaac
      421 tggttgttaa agagataaat aattacccgg ataagattgc tgttactgtg gcacttgaaa
      481 ttggcgggca tccgtcgttg ttgttgccac atgtggcgat tagtcttgac cgtactgaag
      541 gtgccacgct ggagttttac gaagctgagg cgaaaaagca ggcgaagcaa tttttcatgg
      601 atgttgctgc cgggttatgt gaaggggatg gtccgttacc ggaaaagcgt cccgtaattt
      661 tagaggcgca ggatgtgttg ataacctaca gaggaaaact accgggaata attacgggtt
      721 ctctgaagac tccaccgctg gcctgaagac ttaacatatc cagggatttg aaatcgataa
      781 accctgataa atatccatga acgcaaaaat cagatacggc ctgtcggctg ccgttctggc
      841 gctgattgcc gcaggtgcgc ctgcgcctga aatcctcgac cagtttctgg atgaaaagga
      901 aggtaaccac accacagcat accgtgatgg tgcgggtatc tggaccatct gccgtggagc
      961 cacccgggtg gatggtaagc ctgttattcc tggcatgaag ctgtcgaagg aaaaatgcga
     1021 ccgggttaac gccattgagc gtgataaggc gctggcatgg gtggagaaaa acatcagagt
     1081 gccactgacc gaaccccaga aagcggggat tgcgtcattc tgtccgtaca acattggccc
     1141 cggtaagtgt ttcccgtcga cgttttacag acggattaat gcaggagatc gaaaaggtgc
     1201 ctgcgaagct attcgctggt ggattaagga cggtggcagg gactgccgta ttcgctcaaa
     1261 taactgttac ggtcaggtat cccgtcgtga ccaggagagc gcgctggcgt gctggggaat
     1321 cgacagataa gcagaatatt ttgctgaaaa atgaggtttg cttacatgga tggataacac
     1381 gaaatcctgc aaattggcaa aatgtaagtg aataaagtca aaacagttgt ttaacactca
     1441 ggcaccgtaa tgatgccttt gtcatttctg cgcatctcac gcgcatctca caacacagaa
     1501 cctttcagga tgacccttga ggataccggt ttggctgtcg gtgcctttct gtgggctgga
     1561 ttcctgtgag acaaggttca tcactaaaag gaaataaccg atgaatatga tggccgtgcc
     1621 gtttcacggc aactctcttt atgtagttaa ccataatggc gaaccatacg ttcccatgaa
     1681 acctgtcgtt gcggggatgg ggctggcctg gcaatcacag ttggctaagt taagacagcg
     1741 ttttgcgtca actataacgg aaatcgttat ggttgctgag gatgggaaac aacgcaatat
     1801 ggtgtccatg ccacttcgaa aacttgccgg ctggctacaa accattaatc ccaacaaagt
     1861 aaaacccgaa atccgcgata aggtcatccg gtatcaggaa gagtgcgacg atgttcttta
     1921 cgagtactgg acgaagggtt ttgtcgttaa tccccgtaaa atgagcgtga tggaagaact
     1981 caaccaggct tgtgctgaca tgaaacggga taaaaacatt gccagtgtgt ttgctaccgg
     2041 gctgaatgag tggaaacagg ttaaagccgc gcatgtatca aaaatccgta cgctggtaaa
     2101 tgaagcga
//
LOCUS       DAZFWL020000323         2084 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFWL020000323 DAZFWL020000000
VERSION     DAZFWL020000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.327"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            296..421
                     /locus_tag="ACHKNP_RS26145"
                     /old_locus_tag="pgaptmp_005229"
                     /pseudo
     CDS             296..421
                     /locus_tag="ACHKNP_RS26145"
                     /old_locus_tag="pgaptmp_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(481..987)
                     /gene="yciE"
                     /locus_tag="ACHKNP_RS26150"
                     /old_locus_tag="pgaptmp_005230"
     CDS             complement(481..987)
                     /gene="yciE"
                     /locus_tag="ACHKNP_RS26150"
                     /old_locus_tag="pgaptmp_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU4707181.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1033..1533)
                     /gene="yciF"
                     /locus_tag="ACHKNP_RS26155"
                     /old_locus_tag="pgaptmp_005231"
     CDS             complement(1033..1533)
                     /gene="yciF"
                     /locus_tag="ACHKNP_RS26155"
                     /old_locus_tag="pgaptmp_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU4707182.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(1619..1798)
                     /gene="yciG"
                     /locus_tag="ACHKNP_RS26160"
                     /old_locus_tag="pgaptmp_005232"
     CDS             complement(1619..1798)
                     /gene="yciG"
                     /locus_tag="ACHKNP_RS26160"
                     /old_locus_tag="pgaptmp_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGU4707183.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT          562 a          417 c          431 g          674 t
ORIGIN      
        1 ctcttcaagg atggtgatcc tcaaccgcac ccatttagga tgcgactatt gtggtagaac
       61 aatgtaaacc ataattaatc tgcgtgcgga tacatgcttc atagatagga tattctggtt
      121 tgggttgtga atgtgttggt catcgagtgg acaataaggt aagaggagta actcctgcaa
      181 aacaaaaaaa gagttcaaca aaatcaatca gttaaaaaca cgagagatct gtataaaaaa
      241 gcatcgtaaa ttctgtattt ttcctttaaa tataatatgt tattgagttt tgtaaaatct
      301 accagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg gtgcacagca
      361 acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat atcgttttta
      421 attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt ataaagggta
      481 ttatttcttc gcttctacgc catcagtttc agaacgaatt aaaaatttct cagttgtttg
      541 cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa tcgcttcgat
      601 gatcggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg aggtgtaaca
      661 ggcgatttca aattgctcga agacatatcc gctaatagag cctttaacta tttcatcaga
      721 agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg aatctttaat
      781 gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa tctggttttt
      841 ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat ctatacggct
      901 ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt cacgtaacca
      961 gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg actaaaagtt
     1021 aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa gggccagatc
     1081 agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa gcttcgctgc
     1141 tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg caatctcata
     1201 atgctcgact ttctgcgctg cggcaatcag tgcggcatca cgcacttcgt ttttctcggt
     1261 actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac atttcatgcg
     1321 cttaattttc aggttcgatt cggactccac aacttgatca atacgttcaa tctgtccatg
     1381 agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg ttgctcttgc
     1441 gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg aaagcaggtg
     1501 aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa tcactgcgag
     1561 ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta acggtgaatc
     1621 aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc gcttcagatg
     1681 cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta cggcctgcgt
     1741 cggatgcctt ctcacggtct tcggcgaaat ttcctgaacc accacgatgt tcggccatgt
     1801 tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc gacactcatt
     1861 aaaattagtc gctaatgaga attagtcaaa ttaagcgcaa cgagaagata gagggaaaat
     1921 atattttgag gaacattctg gatatattaa caattacctg aggaataagt gacttagaga
     1981 aaattattga ttttactggt gttatgttgc gggattaatt tgtttgatta tattactgtt
     2041 gggcggaaaa atgacgtaag ttgacgctcg accggggtaa gcga
//
LOCUS       DAZFWL020000324         2081 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFWL020000324 DAZFWL020000000
VERSION     DAZFWL020000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.328"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..276
                     /locus_tag="ACHKNP_RS26165"
                     /old_locus_tag="pgaptmp_005233"
     CDS             <1..276
                     /locus_tag="ACHKNP_RS26165"
                     /old_locus_tag="pgaptmp_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4707184.1"
                     /translation="VWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGD
                     LAIVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
     gene            328..711
                     /gene="gpFI"
                     /locus_tag="ACHKNP_RS26170"
                     /old_locus_tag="pgaptmp_005234"
     CDS             328..711
                     /gene="gpFI"
                     /locus_tag="ACHKNP_RS26170"
                     /old_locus_tag="pgaptmp_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4707185.1"
                     /translation="MATKEENQKHLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            704..1057
                     /locus_tag="ACHKNP_RS26175"
                     /old_locus_tag="pgaptmp_005235"
     CDS             704..1057
                     /locus_tag="ACHKNP_RS26175"
                     /old_locus_tag="pgaptmp_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4707186.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSCPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            1072..1605
                     /locus_tag="ACHKNP_RS26180"
                     /old_locus_tag="pgaptmp_005236"
     CDS             1072..1605
                     /locus_tag="ACHKNP_RS26180"
                     /old_locus_tag="pgaptmp_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707187.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMPGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            1602..1997
                     /locus_tag="ACHKNP_RS26185"
                     /old_locus_tag="pgaptmp_005237"
     CDS             1602..1997
                     /locus_tag="ACHKNP_RS26185"
                     /old_locus_tag="pgaptmp_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4707188.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALAGMITTM
                     VQQGYDYRRDDDMALWSSADLTYSITYEM"
     gene            2005..>2081
                     /locus_tag="ACHKNP_RS26190"
                     /old_locus_tag="pgaptmp_005238"
     CDS             2005..>2081
                     /locus_tag="ACHKNP_RS26190"
                     /old_locus_tag="pgaptmp_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707189.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYTGKGDA"
BASE COUNT          461 a          483 c          666 g          471 t
ORIGIN      
        1 gtctggcgtc tgctgaatgg ttttaagctg ttccgcgaaa aactggatac ccgtcgcggc
       61 tcaaattcac aactcgaaac ggcagtgaaa gacctggggg cggtggtgtc tttcaaaggg
      121 tattacggcg atctggccat tgtggtggcc tcttcccgtt atccgaaaca ctggctgacg
      181 gtaggggatc ccgcccgtga atttaccatg acgcagtccg cgccgctgat ggtgttgccg
      241 gacccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcggggg cgaaatgccc
      301 ccgtgtcttt tttcacaggg ggctgatatg gcaacaaaag aagaaaatca gaaacatctt
      361 cgtcaactgg ctggcctgct ggggcgcgag gcggatatgt cggggagtgc tgcggatatt
      421 gcgcaacgtg tgtctgagtg ggaagaggag cttgctgctt cccgggaggg cattatgcct
      481 ggtgatgaga gcgggcctga gcaaaatcac acagacgatg gtgagcagtt gcacaacact
      541 gatgctacgg atgatgttaa agcggtccgt gtgcggaaat gcctgcatgt gatggggtat
      601 tgcccggaga caggccgtcc cgttgaactg acgtaccggg gcatgcgtgt tctggtgcca
      661 tcaccactgg cgacagccat gatacagcac ggaacggctg agcatgcgtg attttcagaa
      721 tgcctttgat gctgcccttg ccggggtgga cagcacgatt gttgaagtga tggggctctg
      781 tgcgcagttc acctcggggg cacagtgtgg cagcgaagtt cagggggttt ttgacgatcc
      841 ggagtcgctg gggtttgccg gtagcggggt ccgtattgaa ggaagctgcc cgtcattatt
      901 tgtacggacg gatacggttc gtgctgtgcg gcgtggtgac acgctgacca ttaatggtga
      961 gacattctgg gtggatcgtg tttctccgga tgacgggggc agttgttatc tctggctcaa
     1021 ccgtgggcaa ccaccggcag ttaaccggcg acgataaacg cagggtgaat tatggcgata
     1081 aaagggcttg atcaggcgat tgaaaatctg agccgggttc gtaaaaacgc cattccggcg
     1141 gcttcagcaa tggccattaa ccgcgtggcc acaacggcga ttaatcagtc ttcgtcacag
     1201 gttgcccggg agaccagggt gagccggaaa ctggtaaagg aacggtccag actgaaacgg
     1261 gccacggtca gaaatccgaa tgccagaatt atcgttaacc gcggtgatct cccggtgatt
     1321 aagctgggga tcaggatgcc ggggcgtcgt ccggacagca tactcaaagc cggtcagcat
     1381 cggtatcagc gggcatttat tcagcgatta aaaaatggtc gctggcatgt catgcagcgt
     1441 gtggccggga aaaaccgtta ccccattgat gtggtgaaaa tcccgatggc ggccccactg
     1501 aaacaggcgt ttgatgagaa tgttgaccgt atccggcgtg aacgcctgcc cggagaactg
     1561 gcatatgcgc tgaaacaaca actgaggatt gcgataaaac gatgaaacat actgatatcc
     1621 gtgctgcagt gctggatgca ctggagctgc atgaacacgg ggcgacgctg tttgatggtc
     1681 gccccgttgt ttttgacgaa gaggattttc ccgcggtcgc ggtttatctg acggatgcag
     1741 agtataccgg tgaagagctg gatgcggata cctggcgggc cacactgcat attgaagttt
     1801 tcctgcctgc tcaggtgccg gattcagagc tggattcgtg gatggagtcc cggatttatc
     1861 cggcgatgtc cgcgatccct gcactggcag ggatgattac cacgatggtt cagcagggct
     1921 atgactatcg tcgtgatgac gatatggcgt tatggagctc tgcagatttg acttattcca
     1981 ttacatacga gatgtgagga cgatatgcca acaccaaatc ccctggcgcc ggtaaaaggt
     2041 gccggtacca ctctgtgggt ttacaccggc aagggtgatg c
//
LOCUS       DAZFWL020000325         2078 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFWL020000325 DAZFWL020000000
VERSION     DAZFWL020000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.329"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..224)
                     /locus_tag="ACHKNP_RS26195"
                     /old_locus_tag="pgaptmp_005239"
     CDS             complement(<1..224)
                     /locus_tag="ACHKNP_RS26195"
                     /old_locus_tag="pgaptmp_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HGU4707190.1"
                     /translation="MKKISFFIFTALFCCSAQAAPSSLEKRLGKNEYFIITKSSPVRA
                     ILNDFAANYSIPVFISNSVNDDFSGEIKNE"
     gene            complement(224..679)
                     /gene="sepD"
                     /locus_tag="ACHKNP_RS26200"
                     /old_locus_tag="pgaptmp_005240"
     CDS             complement(224..679)
                     /gene="sepD"
                     /locus_tag="ACHKNP_RS26200"
                     /old_locus_tag="pgaptmp_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HGU4707191.1"
                     /translation="MNNNNGIVKNDSDWLTALDFVKDVKGSPKHLTFHIYQKNAFLHD
                     FGSYWVLYIELNGDFRQVPTDTFIRLCNILAVSKEYKQMGIFQSNKKWYLCQLFNKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQASTSDTTMPPTHLFSDIGRIV"
     gene            complement(685..1257)
                     /gene="escJ"
                     /locus_tag="ACHKNP_RS26205"
                     /old_locus_tag="pgaptmp_005241"
     CDS             complement(685..1257)
                     /gene="escJ"
                     /locus_tag="ACHKNP_RS26205"
                     /old_locus_tag="pgaptmp_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HGU4707192.1"
                     /translation="MRKYIKNTFLFVFICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNVTISVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            complement(1259..1636)
                     /gene="sctI"
                     /locus_tag="ACHKNP_RS26210"
                     /old_locus_tag="pgaptmp_005242"
     CDS             complement(1259..1636)
                     /gene="sctI"
                     /locus_tag="ACHKNP_RS26210"
                     /old_locus_tag="pgaptmp_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HGU4707193.1"
                     /translation="MNNINQSENINIHFNKVSQTNFVDDHTPLAPTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            complement(1720..2022)
                     /locus_tag="ACHKNP_RS26215"
                     /old_locus_tag="pgaptmp_005243"
     CDS             complement(1720..2022)
                     /locus_tag="ACHKNP_RS26215"
                     /old_locus_tag="pgaptmp_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepZ/EspZ family type III secretion system
                     protein"
                     /protein_id="HGU4707194.1"
                     /translation="MDAANLSPSGGVLPLAATINGNVSVDENTGVMIPENGVSRNIRI
                     AAGIALATTALAALGTGIAMACTTDASSQEFLGLGIATGVLGGVTALGGGLAMKYA"
BASE COUNT          599 a          390 c          383 g          706 t
ORIGIN      
        1 ttttcatttt ttatttcacc agagaaatca tcattcacag aattactgat gaaaacaggg
       61 atgctataat tagcggcaaa atcattgagg attgcacgta ccggagagct ttttgtaata
      121 ataaaatact cgttcttgcc taatcttttt tccagagagg acggggcagc ctgagcactg
      181 cagcaaaata gtgctgtaaa aataaaaaaa cttatttttt tcattacaca attcgtccta
      241 tatcagaaaa caaatgtgtt ggaggcatcg ttgtatctga tgtagatgct tgctctaact
      301 tatcaaattg cttgtcaagc aaagaggcga gtgtgtgttg cattattgct tttgacatgt
      361 ttgcgcgatg attattatct ttattaaaca gttggcaaag ataccatttt ttattagatt
      421 gaaaaatgcc catttgttta tattctttac taacggccag aatattacag agtctgatga
      481 aggtatctgt tggaacctgg cgaaaatcgc cattaagctc gatatacaga acccagtaac
      541 ttccaaagtc gtgtaagaag gcattttttt gatagatatg aaaagtaaga tgctttggtg
      601 aacctttgac atcttttaca aaatcaagag ccgttagcca gtcactatca ttcttaacaa
      661 tgccattatt attgttcata catattaccc gtcctgtcct gaggatgact tgataacgac
      721 actaatattt tctagtttga gatcgtctac gctattctta actaagtttt ttatctgaat
      781 gactgatggt gctaagttaa cctctggcga gctaatgact aaaacagccg ctgaggatgg
      841 ttggctctcg ttattgttaa catttaatga aacgctgcaa tcgataacac cggggatttt
      901 acttagtaac ctttctatat cttgttcttt gagatagttg atttttgcat tttcttgtga
      961 tggcgatgcc actagctgac tgggcggaaa aataacttca atatctgcaa atttcttttt
     1021 cgggaagcca ttgttattta aaatggttat cgcacggaca aaatcctctt tttccacact
     1081 tattgttaca tttccacttt tgtccatttc tttagataca ttgacatcat tggataacaa
     1141 caatgcttgc atctgattgg cttctttttc cgttaatcct gtatacaatt gctctttaca
     1201 tgcaactgtc aaacaaataa aaacaaataa aaaggtgttt ttaatgtatt ttctcatttt
     1261 attgcattga aaccagtttt gtaaccgatg ttgaaagaag acttgctaac ttggatacga
     1321 catcaacatt gacgtttgcg ttgagcacat tcttttgtaa tgtgagcaca tcttctggcg
     1381 agagtccacc tgcagatagg gcatcgaaac tgagatcggt gttcattgtt gcaagatgtc
     1441 tctttttaat ttcttcaatt aaaacttgct ctggagaaga tacaccagct gttttaggaa
     1501 gtaattgatc aagaaactga gccgctccag ccgcagaggg cgttggtgct aacggggtat
     1561 ggtcatcaac gaaatttgtt tgggatactt tattaaaatg aatattaata ttttctgatt
     1621 gattaatatt gttcatattt acggttagtc tcttttcatg agagaggtaa tggcataatg
     1681 catccatgac ctctctattt tatttatgcg atgcgatgat taggcatatt tcatcgctaa
     1741 tccgccgcca agcgcagtaa caccacctaa tacaccggta gcaataccta aacctaagaa
     1801 ttcttgggag cttgcatctg ttgtgcatgc cattgcgata ccagtaccta aagcagccag
     1861 agctgtagta gcgagtgcaa ttccggctgc aattctgata ttacgactta ccccattttc
     1921 tggtatcatt actcctgtat tttcatccac cgaaacattt ccattgattg tggctgccag
     1981 tggtaatact ccgccagaag gacttaaatt tgctgcatcc attaactttt ctccattgtc
     2041 taatttagac atttgtctgg ttatcaataa ccaaatta
//
LOCUS       DAZFWL020000326         2077 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFWL020000326 DAZFWL020000000
VERSION     DAZFWL020000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.330"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1230
                     /locus_tag="ACHKNP_RS26220"
                     /old_locus_tag="pgaptmp_005244"
     CDS             <1..1230
                     /locus_tag="ACHKNP_RS26220"
                     /old_locus_tag="pgaptmp_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4707195.1"
                     /translation="RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRL
                     TTIQNDRTRIQTIYQPGSFTPLIRVETATGEQAKTQRRSLADTLQQSGGEDGGSVVFP
                     PVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDH
                     RGLPLALVSTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHR
                     YYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKP
                     PTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCT
                     PQDDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDL
                     GEILAKMKQRTSRGIRK"
     gene            1227..1796
                     /gene="ybfC"
                     /locus_tag="ACHKNP_RS26225"
                     /old_locus_tag="pgaptmp_005245"
     CDS             1227..1796
                     /gene="ybfC"
                     /locus_tag="ACHKNP_RS26225"
                     /old_locus_tag="pgaptmp_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HGU4707196.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHELIRSEEK"
BASE COUNT          633 a          443 c          507 g          494 t
ORIGIN      
        1 cgctatcttt acgacccgct gggccgcagg gtggcaaaac gggtgtggcg acgtgaacgg
       61 gacctgacgg gctggatgtc gctgtcacgg aaaccgcaag tgacctggta cggctgggac
      121 ggcgaccgcc tgaccacaat acagaacgac agaacccgca tccagacgat ttatcagccg
      181 gggagcttca cgccactcat cagggttgaa accgccaccg gtgagcaggc gaaaacgcag
      241 cgccgcagcc tggcggatac ccttcagcag tccggcggcg aagacggtgg cagtgtggtg
      301 ttcccgccgg tgctggtgca gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg
      361 gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc tgacggtgga gcagatgcaa
      421 aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat
      481 cgcggcctgc cgctggcgct tgtcagcacg gaaggggcaa cagaatggtg cgcagaatac
      541 gatgaatggg gcaacctgct gaatgaagag aacccgcatc agctgcagca gcttatccgc
      601 ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
      661 gacccgctgc aggggaggta tatcactcag gatccgattg ggctgaaggg gggatggaat
      721 ttttatcagt atccgctgaa tccggttcag tatatagatt caatgggact ggcatcaaaa
      781 tatggacact taaataatgg cggatatgga gcgagaccca acaaaccgcc tacgcccgat
      841 ccaagtaaat tgccggacat agcgaaacaa ttaagactgc catatcctat tgaccaggcc
      901 agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta cgactacaca
      961 ctgtattgca ggaagtgggt aaaaccaaat ctgacttgta cgccacagga tgattcccag
     1021 tatccaggga tggatacaaa gacagcaagt gattacctgc cacagacaaa ttggccaaca
     1081 actcaattac caccaggata tacttgtgca gaaccctatt tattcccaga cattaataaa
     1141 cccgatgggc cagcaacagc agggatagat gatttgggtg aaattttagc taagatgaaa
     1201 cagagaacat cgagaggaat aagaaaatga aaagagtttt gttctttttg ctgatgatat
     1261 ttgttagttt tggtgttata gctgattgcg aaatacaagc taaagatcat gattgtttta
     1321 ctattttcgc taagggtact attttttccg cgtttcctgt cttaaataat aaagccatgt
     1381 ggagatggta tcagaatgag gacatcggtg agtattattg gcaaacagaa ctgggtacat
     1441 gtaaaaataa taaattcaca ccaagcgggg cgagactctt aattcgcgtc gggtcactac
     1501 gccttaatga aaatcatgcc atcaaaggaa cgttgcagga gttgataaat acagcagaaa
     1561 aaacggcgtt tctcggcgat cggtttagaa gttatataag agcgggtatc tatcaaaaaa
     1621 agagcagtga tccagttcag ttgttggcag tccttgataa ttcaatcatg gtaaaatatt
     1681 ttaaagatga gaaaccaact tatgctcgaa tgacagcaca tctcccgaac aaaaatgagt
     1741 cttacgaatg tttaataaag atacagcatg aactaattcg tagtgaagag aaataaaatc
     1801 tcttcctgag aaaacaaatt aatcattgtg aaaaattata taactcatgg aataggtgtt
     1861 tattttgcgt tccatttgca gggaaagatc acgtaacgct acttttttgt actaaataat
     1921 tcgaatttta agttcaacaa ttgagatact acttattgtc taaagctgtt tttcatagct
     1981 tatacatgat caaatactcc ttacataaat aaggtgaaca aatggaactt aaaaaattga
     2041 tggaacatat ttctattatt cccgattaca gataagc
//
LOCUS       DAZFWL020000327         2042 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFWL020000327 DAZFWL020000000
VERSION     DAZFWL020000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.331"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            189..941
                     /locus_tag="ACHKNP_RS26230"
                     /old_locus_tag="pgaptmp_005246"
     CDS             189..941
                     /locus_tag="ACHKNP_RS26230"
                     /old_locus_tag="pgaptmp_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HGU4707197.1"
                     /translation="MTTVNQIPDANITPSLPPKIREAVDKVKAAKAVWQEERRKQTEA
                     AAMIETIRKRQEDTKTETQALNDEWRNLFRENQGNMTPRMKKLRAEIALGRETLDEFE
                     DLLAAQAAENEFLPWKTADAANHYISEHNRLIETHAVWLWNEFMKEHGQKLIQILGLL
                     KMTLGRSASAVIGVVHTVNDPESVLKQFISEQLTTPALSCNVSSTDDITLPGISIYAD
                     DKAIQDARQSPSPAARSRMLKQRDMVKGGEKK"
     gene            938..1492
                     /locus_tag="ACHKNP_RS26235"
                     /old_locus_tag="pgaptmp_005247"
     CDS             938..1492
                     /locus_tag="ACHKNP_RS26235"
                     /old_locus_tag="pgaptmp_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HGU4707198.1"
                     /translation="MNAGTITQEALNDYRSAIKSWLTLRNTQSTYQLRLAALLDTEEK
                     PAAYASQLEDLRERLAVLEWQINCAARDGLYAHQIVLESCVTSATENFMSEHGDALTD
                     ALAPFLCAPYGLEAAMKILRTAVARQTEMRTPVISAAYKSIIDETGLTVDASMRADAS
                     ASFTPAQHKVFLARLNRLNEKGVC"
     gene            1494..1766
                     /locus_tag="ACHKNP_RS26240"
                     /old_locus_tag="pgaptmp_005248"
     CDS             1494..1766
                     /locus_tag="ACHKNP_RS26240"
                     /old_locus_tag="pgaptmp_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HGU4707199.1"
                     /translation="MALKCPECGAVAHARTSAYEAPSVKRSWYQCQNLECSCTFTALE
                     SVDTIIMKPRCNEQESDKAKIPEKQQQTLNRYGSASKLSNRQQIPV"
BASE COUNT          584 a          494 c          507 g          457 t
ORIGIN      
        1 atatttttat tgaagtgaac agtagtgaat agtatgtatt aaaggaagcg tgtagatcag
       61 acatgacacc tttctctggc tagccagaac aaggtcattg ttcagtcact gacacaatcc
      121 tcctcggtag tgcgcttgta tggaccccgg cacaattgac acaacaacaa aacactaccg
      181 gagcagtcat gactactgtt aaccagatcc ctgatgcaaa cattacaccc tccctcccac
      241 cgaaaattcg cgaagcggtg gataaagtta aggcagcaaa agctgtctgg caggaagaac
      301 gacgaaaaca aactgaagct gctgcaatga ttgaaactat ccgtaaacgt caggaagata
      361 caaaaacgga aacgcaggcg cttaatgatg aatggcgaaa cctgtttcgt gagaatcagg
      421 gaaatatgac gccacgcatg aaaaaactgc gggcagaaat cgccctcgga cgcgaaacac
      481 tggatgagtt cgaagatttg ctggcagctc aggcggcaga aaatgaattc ctgccctgga
      541 aaactgcgga tgctgcaaac cactacatca gcgaacataa tcgcctgatt gaaactcatg
      601 cagtgtggct ctggaatgag tttatgaagg aacacggtca gaaacttatt cagatccttg
      661 gactgctgaa aatgactctg ggccgaagcg cttctgctgt tatcggtgta gttcataccg
      721 taaacgaccc cgaaagtgtg ctgaagcaat ttatcagcga gcaactcacc actccggcac
      781 tgtcctgtaa cgtatcttca accgatgata ttaccctgcc ggggatcagc atttatgcgg
      841 acgataaagc catccaggat gccagacaat cccccagccc tgcagcgcgt tcacggatgc
      901 ttaaacagcg tgacatggtt aaagggggcg agaagaaatg aatgccggaa ccattactca
      961 ggaagcactc aacgattacc gctcggcgat aaaaagctgg ctgacactac gtaatacgca
     1021 aagtacctac cagcttcgtc tggcggctct gctggatact gaagaaaaac cggcagcata
     1081 tgccagccag cttgaggatc ttcgtgagcg tcttgcagtc ctcgaatggc agattaactg
     1141 tgctgcacgg gatggtcttt atgctcatca gattgtgctg gagagttgtg ttacaagcgc
     1201 aacggaaaac ttcatgagcg agcatggtga tgcactcact gacgctctgg ctccttttct
     1261 ttgcgctcca tacgggcttg aggcggcaat gaaaatatta cgaaccgctg tagcccgaca
     1321 aacggaaatg cgtactccgg taatttcagc agcatataag agcattatcg acgaaaccgg
     1381 attaacggtg gatgcatcaa tgcgcgctga tgcttcagcc agtttcaccc cggcacaaca
     1441 taaagttttc ctggcccgcc ttaaccgact taatgaaaaa ggagtgtgct gatatggccc
     1501 tgaagtgtcc tgaatgtggc gcggtcgctc acgccagaac cagcgcctat gaagctccgt
     1561 cggttaaacg ctcatggtat cagtgccaga atctggaatg ttcctgcaca tttaccgcac
     1621 tggaaagcgt ggatacgata attatgaagc ctcggtgcaa tgaacaggaa tcagacaaag
     1681 caaaaatacc ggaaaaacaa cagcaaactc tcaatcgcta tggctccgcg tcaaagctgt
     1741 caaaccgtca gcaaattcct gtctgattag caaaatacga ccacgaagtc ccggtccagt
     1801 accgggattt tttacgcctt tttcctggct ggcctgagag cgtatgagtg catatctatg
     1861 gcgcatgaaa acgcatgagt ctcatgcgcc atttttgacg cgaaagccct tgtgtggtgg
     1921 cttctgagac gatttacggg gtgcatgaaa accagtctgt taagcgaagc gggcaggcgg
     1981 gcggggcatt gcgcgcagga atgtgtatac cgttttagta atgatcgctg acatctcact
     2041 tt
//
LOCUS       DAZFWL020000328         2042 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFWL020000328 DAZFWL020000000
VERSION     DAZFWL020000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.332"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..105
                     /locus_tag="ACHKNP_RS26245"
                     /old_locus_tag="pgaptmp_005249"
     CDS             <1..105
                     /locus_tag="ACHKNP_RS26245"
                     /old_locus_tag="pgaptmp_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707200.1"
                     /translation="DEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            413..529
                     /locus_tag="ACHKNP_RS26250"
                     /old_locus_tag="pgaptmp_005250"
     CDS             413..529
                     /locus_tag="ACHKNP_RS26250"
                     /old_locus_tag="pgaptmp_005250"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707201.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            748..>2042
                     /locus_tag="ACHKNP_RS26255"
                     /old_locus_tag="pgaptmp_005251"
     CDS             748..>2042
                     /locus_tag="ACHKNP_RS26255"
                     /old_locus_tag="pgaptmp_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HGU4707202.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     FSSACQNQIDVCRPGNLRGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKLHKEIRAGKDCKATESELKRHLNKCPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGIIFDGRAMSKLGILNSLWDGGSLFIDRKSS
                     PGIILKDPRLTVSVMIQPDVYQKGFCTRKKELVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEQRINELIDENLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIHHSSGEEGDISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNPALTVPVRLIPEELELFNWIKSYCIEKGIPCMKKN"
BASE COUNT          647 a          369 c          477 g          549 t
ORIGIN      
        1 gacgaatggc ttactttaca tactgtacct gccagatcag cagtcaaagg tgttaatgac
       61 gaagtgcgac atacgctttc ttcagatatg accatgagca gataagtttt tgtcatcacg
      121 tgatcacctc aggaggaaaa agaagcataa gaacaattta gccagaagtt taattgataa
      181 acagaatttc cagcattcat tgttgaaaga gcagctattg tcgctattgt agcggtaatt
      241 gctgctataa ccgaaacgta ttacgaaaaa ggaagcattt atgtcttaca tcaaaccgga
      301 tatgccgtat tcagtatatg cgtgaccatc aatgataggg aaggcaataa ttaaagctca
      361 atacaataaa caagtacctc tgactctggt tgcaatgtca gtattaacag cgatgtcaat
      421 tgcctgtcag aatcaggttg acgtatgcag ccccggaaat ttacgtgggg ccgttaatat
      481 ttacactatg gttctggaga aagggaaaag accgggggaa aggtattagt gaaataattt
      541 caggaggtat taagaatgag aaatatgaat gatgtttcgt ggtaaataat acagtctgca
      601 ctgaattaac cttatcggtt tattaaacat cagatctaca aatgctctgt atttctcaga
      661 gcatcaggtc tgaatagcag ctattttcgc tattgtagcg ggatttgctg ctattttact
      721 aacccattac tgaaaagaga ggcttttatg tgcttacttg caccagaaaa tccttatccg
      781 atatatgcac taccaccgct ggtgagaaat gcaataattg aaactcaaaa aaatacacag
      841 gcacctttgg ctatggtggc gacatccgca ttaacagcgt tctcaagtgc ctgtcagaat
      901 cagattgacg tgtgcagacc tggaaattta cgtgggcctg ttaatcttta ctctctgatt
      961 ctggctgatt ccggtgaaag gaaaacgacc gtggataaag tgtttatgaa agcattttat
     1021 ctcagggatg aagccctggc ggaagaatac gcgaaactgg ttgagaatta cagtacagaa
     1081 aaggaaatat gggagcaaaa gcaaaaagca ctggagtcaa aattgcataa agaaattcgc
     1141 gccggtaaag attgtaaggc aacagagtca gagcttaaaa gacatctgaa taaatgtcct
     1201 gttccgccgc agatacgccg gacaattttc aatgagacaa cgatagaggg aatgttaaaa
     1261 tattattccg acagcaatcg ctcttttgct cttgtatcca gtgaaggagg aataattttt
     1321 gatggccggg caatgagtaa acttggaata cttaactctt tgtgggatgg tggttctctt
     1381 ttcatcgaca ggaaatcatc tcccggaatt attttgaagg atccaagact gacggtatcg
     1441 gtgatgattc agcctgatgt ttaccagaaa ggtttttgta cgcgaaaaaa agaacttgtg
     1501 aaaacgtcag gacatcatgc aaggtttttg atgtgtcaac caacatcaac gcaggggaca
     1561 aggataataa ccggagataa ttattcatca cagtatcagg atttgtttga acagaggatc
     1621 aatgagctta ttgatgaaaa tctggcgatg agtggtgaac gacgttgcct tcacttttct
     1681 cctcaggctg ccagaatctg gacggattat tacaacgatg tggaatcgaa gctgggggga
     1741 ttggggcctt taagacattg ccgggaatat gctgctaaaa atgcagagta tatggcaaga
     1801 ctggcaggac ttattcacca ttcgagcggt gaagaggggg acatttcccc ttacactgca
     1861 gaaatggcga gagaattagc aatatggtac ggtaatgagt atgtgcggtt gtctaatccg
     1921 ttaacttttg acaaccctgc cctgaccgta cctgtgcgac tcattccaga ggagcttgaa
     1981 cttttcaact ggataaaaag ctattgcatt gagaagggga tcccctgtat gaaaaaaaat
     2041 ga
//
LOCUS       DAZFWL020000329         1995 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFWL020000329 DAZFWL020000000
VERSION     DAZFWL020000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.333"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            170..1522
                     /locus_tag="ACHKNP_RS26260"
                     /old_locus_tag="pgaptmp_005252"
                     /pseudo
     CDS             170..1522
                     /locus_tag="ACHKNP_RS26260"
                     /old_locus_tag="pgaptmp_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1535..>1995
                     /locus_tag="ACHKNP_RS26265"
                     /old_locus_tag="pgaptmp_005253"
     CDS             1535..>1995
                     /locus_tag="ACHKNP_RS26265"
                     /old_locus_tag="pgaptmp_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGU4707203.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSS"
BASE COUNT          652 a          390 c          369 g          584 t
ORIGIN      
        1 aggaataaaa aacaatctga agcatgctct gaaacatcct actctgtcac tctaatgatg
       61 gcaccttgct cttcttataa agaagggaac aattaagaat attaaatctt caaactttca
      121 tcaacttcac atcaagaaat taaaaaattt aaacattaat ctaaataata acaacctgat
      181 cacataatcg ggtacgattt tatccgataa ctataatgct ataactctgg gaaatggttg
      241 gaatacaccg ctgggtgcca tctcttttta cgctgcccga tcgagtagta agttaaataa
      301 tgataccaga cacgaaggta cgagctatca ggtcgcctat aataaatatc tagtacaaac
      361 agccactcga tttagcgtgg ccgcctggcg ttatgcttcg caggattaca ggacattcag
      421 cgaccatctt tacgaaaatg ataaacacta tcatcagagt gattatgatg atttttatga
      481 tattggcaga aaaaatagcc tttctgctaa tattatgcaa cctttatcca ataatctggg
      541 aaatgtatca ttaagtgcgc tttggcggaa ttactggggg cgaagtggaa atgctaaaga
      601 ttaccaattc agttactcca ataactggca acacattagt tatactttct ctgccagcca
      661 atcttatgat gaaaataata aagaagagga gcgttttaat ctgtttatct ccattccttt
      721 ctactggggg gatgatattg ccaaaacacg tcaccaaatt aacttatcga attcgacctc
      781 attttccaaa gatggctatt cctccaacaa tactggaatt actggcatag ccggtgaaca
      841 tgatcagtta aattatggta tatatgttaa tcagcaacaa caaaataatg atacctcgct
      901 tggtacgaat ttaagctgga gaactcccat cgccataata gatggcagct atagtcattc
      961 taaaaacgcc tggcaaagtg gtggaagtat tagtagtgga ttagttgtct ggcccggtgg
     1021 tattaatatc actaaccagt tatccgatac atttgcaatt ctggatgcgc ctgggttaga
     1081 aggtgcacat attaatggac aaaaatacaa tcgaacaaac agcaaaggcc aggttgttta
     1141 cgacctgata atacctcatc gtgaaaacca tctggtactt gatatagcaa acagtgaaag
     1201 tgaaacagaa ttgcagggca atcgtcaaat tattgcacct taccgtggag cagtttctta
     1261 tgtgcagttt acaactgacc aacgtaagcc ctggtatata caggcactgc gtcccgatgg
     1321 ttcgccatta acctttggct atgacgtact ggatctccag gaaaataata ttggagtcgt
     1381 tggccagggt agtcgccttt ttattcgcgt agatgaaatt cccactggca taaaagttgc
     1441 tctcaatgat gaacagaact tattctgtac tattactttt caacacgtta tcgatgaaaa
     1501 caaaacatat atatgccagt aaaaggagtt tactatgaag tggcttttat taatcacctt
     1561 gagtctatat tcttttattg tgcaaagtgc tccctgtgca ttgaccaatg tcggtgaaca
     1621 acgtggaaca tatatactca agccactgag tatgaaaggt aatctaaccg ccgaaaaaat
     1681 tgaaaacacc ggaaatatca ttgagtgtac agacttgcaa gagacaacca acgccattaa
     1741 agttcatttt ttaacacaga acgctttaag gtttaaagtc ggaagtcgtc attatattat
     1801 taatttcccc tttgaatctg acggtaaaaa aatagagctt ccagggaagt catcttataa
     1861 tttagaagaa atgctatctg cgataaattt caccattcca tattctatcg catctgtatc
     1921 agatagttct aacaccgcag acatttttcc tggaactcct tttcctttgt tgttcgaaat
     1981 tgaagtgagt tcatg
//
LOCUS       DAZFWL020000330         1994 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFWL020000330 DAZFWL020000000
VERSION     DAZFWL020000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.334"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(22..327)
                     /locus_tag="ACHKNP_RS26270"
                     /old_locus_tag="pgaptmp_005254"
     CDS             complement(22..327)
                     /locus_tag="ACHKNP_RS26270"
                     /old_locus_tag="pgaptmp_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4707204.1"
                     /translation="MATGSKNAKSQSLTARIPHDVIEGMESVKLDGESNAGFIVTAMR
                     GEIARRQAEGSGENLLISSLNALAQVEKLGIKAAEEIGQLVTVAREELQRRKTKEPE"
     gene            524..724
                     /locus_tag="ACHKNP_RS26275"
                     /old_locus_tag="pgaptmp_005255"
     CDS             524..724
                     /locus_tag="ACHKNP_RS26275"
                     /old_locus_tag="pgaptmp_005255"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4707205.1"
                     /translation="MRNHTTHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RLQSPAGCVMVFAARIRQGVGL"
     gene            721..951
                     /locus_tag="ACHKNP_RS26280"
                     /old_locus_tag="pgaptmp_005256"
     CDS             721..951
                     /locus_tag="ACHKNP_RS26280"
                     /old_locus_tag="pgaptmp_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707206.1"
                     /translation="MSQEITLQQAVERAHQIEVICALAEDYPGMMTDSESGAIIGLLK
                     RLSGEVCVFLIDEQERRTLISNEKKCGGVHVQ"
     gene            941..1390
                     /locus_tag="ACHKNP_RS26285"
                     /old_locus_tag="pgaptmp_005257"
     CDS             941..1390
                     /locus_tag="ACHKNP_RS26285"
                     /old_locus_tag="pgaptmp_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707207.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDIECTAELAQKEEREEYTLFAL
                     ECIRYTATRSLNDAKNILVADCENGGGVMRDDRFNSLKQEFSGVPDDAADALSSMPEL
                     IRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            1383..1616
                     /locus_tag="ACHKNP_RS26290"
                     /old_locus_tag="pgaptmp_005258"
     CDS             1383..1616
                     /locus_tag="ACHKNP_RS26290"
                     /old_locus_tag="pgaptmp_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707208.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLDPVIMELQEIDILHDRYKEQHTGE"
     gene            1622..1921
                     /locus_tag="ACHKNP_RS26295"
                     /old_locus_tag="pgaptmp_005259"
     CDS             1622..1921
                     /locus_tag="ACHKNP_RS26295"
                     /old_locus_tag="pgaptmp_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4707209.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVTIKGVEERRITY
                     RREGYEYDCVMPVYQFRRDFSLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
BASE COUNT          581 a          469 c          478 g          466 t
ORIGIN      
        1 acaccacggc gctgatggtg attactctgg ttctttagtc ttgcggcgct ggagttcttc
       61 acgtgcgaca gtgacgagct gcccgatctc ctcggcggct ttgattccga gtttttccac
      121 ctgcgccagt gcattgagcg aagaaatcag gaggttttct ccgcttcctt ctgcctggcg
      181 gcgggcgatt tcaccgcgca tggcggttac tatgaatccg gcgttgcttt caccgtccag
      241 ttttacggat tccatgcctt caataacatc atgcgggatc cgagctgtca gtgattgtga
      301 ttttgcgttt tttgaacctg tagccatctg taatcctctc aatgaaagtg taagacaata
      361 tacacataaa aagtcttaca taaaagcatt gacatgtaag ccacctataa ataaagttac
      421 ttacaccttg ttaatgcaag gtgcagaaac gacgaaaccc cgcactgtag gagcagtaac
      481 ggggcttcta accaccaacg ataacgagag tatcgaggta gctatgagaa atcataccac
      541 acacccgcaa gggcgggact cgcacaacct gaataaatac atttggcgtt ttatcgccct
      601 gagcactgca caaccgcgcg tgattaccat tgaggccacc agcgaacagg aagcacgcct
      661 gcaatccccg gctggctgcg tgatggtatt cgctgcccgt attcgtcagg gggtaggctt
      721 atgagccagg aaatcacact acaacaggca gtagaacgcg cccaccaaat cgaagttatt
      781 tgcgcactgg cagaggatta cccaggcatg atgaccgaca gcgaatcagg ggcaatcatc
      841 ggcttactta aacgccttag cggtgaggtc tgcgtattcc tgatcgatga acaggaaaga
      901 agaacgctta tttctaacga aaaaaaatgc ggaggggtac atgtgcaata aaaccacacc
      961 ggacgcagcc gccgccgcac tcactacgct gatgcacgcg cttattgata ttgaatgcac
     1021 agcagagctt gcacaaaagg aagaaaggga agaatacaca ctattcgccc tggaatgtat
     1081 ccgatacacc gcaacgcggt cgctgaatga cgctaaaaat attcttgttg ctgattgtga
     1141 aaatgggggg ggggttatgc gtgatgatcg ttttaattcc ctgaaacagg aattttccgg
     1201 cgttcctgat gatgcggctg atgcgctttc gtcaatgcca gaacttatta gagcggcttt
     1261 tttcttactt tccacgagag aatataaatc aacggggctt gatgtactga atatcgccgc
     1321 cgattatgcg gaatatgtgg cagaggcgcg ttatagaaga aaatttcctg aggatgtaag
     1381 ccatgcgtga tatttaccac caaacaatag accgcgcatt tcttgcactt tctcacagtg
     1441 aaaacatgct ggaaatattg cgcatatggc ttgaaacact tggcgacaat gaacgcgaca
     1501 aacaaaaatc aagaattgcc acggcattaa taacgcttct tgatcctgta ataatggaac
     1561 tgcaagaaat agatatattg cacgacagat ataaagaaca gcacaccgga gaataaaaat
     1621 aatgaaactt aaatattctg gcttaactgc cagtggcaac actcacccta aatttacgcg
     1681 cggcgatatt taccgcgacc agtacggcgg caccgtaacg attaaaggtg tagaagagcg
     1741 acgcattacc taccgccgtg aaggctacga atatgactgc gtgatgcctg tttatcagtt
     1801 ccggcgtgat ttttctctgg tacaggcagc gccccgcagt aaaccaacca gcagggagaa
     1861 agcacgcgcc aatattcagg aaataaaaaa gatgcttaac gtattcaggg gcaagaaatg
     1921 aaaagcgcac cgaacttaaa aaaacagcct tacgacaaga tgaccgaagt catcattttt
     1981 gcgggtagtg atgc
//
LOCUS       DAZFWL020000331         1993 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFWL020000331 DAZFWL020000000
VERSION     DAZFWL020000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.335"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            50..151
                     /locus_tag="ACHKNP_RS26300"
                     /old_locus_tag="pgaptmp_005260"
     CDS             50..151
                     /locus_tag="ACHKNP_RS26300"
                     /old_locus_tag="pgaptmp_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707210.1"
                     /translation="MRTYNLNYLLLSQMQKCTCDFLYPAFDLCGGEA"
     gene            148..603
                     /gene="ybcN"
                     /locus_tag="ACHKNP_RS26305"
                     /old_locus_tag="pgaptmp_005261"
     CDS             148..603
                     /gene="ybcN"
                     /locus_tag="ACHKNP_RS26305"
                     /old_locus_tag="pgaptmp_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HGU4707211.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQLQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLIRRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            603..773
                     /locus_tag="ACHKNP_RS26310"
                     /old_locus_tag="pgaptmp_005262"
     CDS             603..773
                     /locus_tag="ACHKNP_RS26310"
                     /old_locus_tag="pgaptmp_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU4707212.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            766..1056
                     /locus_tag="ACHKNP_RS26315"
                     /old_locus_tag="pgaptmp_005263"
     CDS             766..1056
                     /locus_tag="ACHKNP_RS26315"
                     /old_locus_tag="pgaptmp_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HGU4707213.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            1053..1415
                     /gene="rusA"
                     /locus_tag="ACHKNP_RS26320"
                     /old_locus_tag="pgaptmp_005264"
     CDS             1053..1415
                     /gene="rusA"
                     /locus_tag="ACHKNP_RS26320"
                     /old_locus_tag="pgaptmp_005264"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HGU4707214.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1412..1552
                     /locus_tag="ACHKNP_RS26325"
                     /old_locus_tag="pgaptmp_005265"
     CDS             1412..1552
                     /locus_tag="ACHKNP_RS26325"
                     /old_locus_tag="pgaptmp_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGU4707215.1"
                     /translation="MMFEFYMAERLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1638..>1993
                     /gene="quuD"
                     /locus_tag="ACHKNP_RS26330"
                     /old_locus_tag="pgaptmp_005266"
     CDS             1638..>1993
                     /gene="quuD"
                     /locus_tag="ACHKNP_RS26330"
                     /old_locus_tag="pgaptmp_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HGU4707216.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLE"
BASE COUNT          514 a          465 c          524 g          490 t
ORIGIN      
        1 accggagttt gaactcctcc ggtgacttct gcgctaaacg gggacgttta tgcgcacata
       61 caatctaaac tatcttctcc tgtcacagat gcagaaatgc acctgcgatt ttctttatcc
      121 agcgtttgac ctctgcggag gtgaagcgtg aacctcccac aagacggcat caaactgcat
      181 cgcggtaact tcaccgctat cggtcagcag ctccagcctt atttggaaga cggcaagtgc
      241 tttcgcatgg tgcttaaacc gtggcgcgag agacgcagtc tttcccagaa tgcacttagc
      301 cacatgtggt acagcgaaat cagtgaatac cttatccgca gggggaaatc gttcgctacc
      361 gcagcatggg taaaagatgc tctcaaacac acatacctcg gttatgaaac caaggacctg
      421 gttgatgtcg taaccggcga aatcactact atccagtcgt tacgccatac ctccgatctt
      481 gataccggag agatgtatgt cttcctgtgt aaggttgaag cctgggcgat gaatattggc
      541 tgccacctga ctattccgca gagctgcgag ttccagctgc tgcgcgacaa gcaggaggcg
      601 taatggctac accgcttatt cgtgtcatga acggacacat ctacagagta ccaaatcgtc
      661 gtaagcgtaa gcctgagcta aaaccatccg aaataccaac actgctcgga tataccgcca
      721 gcctggttga taaaaaatgg ttgcgactgg cagcaaggag gaatcatggc tgatttgaga
      781 aaagcagcgc gtggtcgcga atgccaggta agaatccctg gcgtatgcaa tggcaaccct
      841 gaaacgtctg tactggcaca tatccggctg gctggactgt gtggcaccgg tattaaaccg
      901 ccagacctta ttgccaccat tgcctgttct gcctgtcacg acgaaatcga ccgccgcacg
      961 cattttgtcg atgctgagta cgcaaaagaa tgcgcgctgg aaggtatggc gagaacgcag
     1021 gttatctggc tgaaagaggg ggtaatcaag gcgtgaatac ctacagcatc acattaccct
     1081 ggcctccgag caataatcgc tattaccgcc ataatcgcgg gcgcacgcac gtcagcgcag
     1141 aggggcaggc ataccgcgat aacgtcgccc gaatcattaa aaacgcaatg ctggatatcg
     1201 gcttggctat gcctgtgaaa atccgcattg agtgccacat gccggatcgc cgtcgccgtg
     1261 acctggataa tctgcaaaaa gccgcttttg acgcactcac taaagcaggt ttctggctgg
     1321 atgatgctca ggtcgttgat taccgcgttg tgaagatgcc tgttaccaaa ggtgggaggc
     1381 tggaactgac catcaccgaa atggggaatg aatgatgttt gagttttata tggcagaacg
     1441 tcttcgccac cgctgggggc gtctgcgctt atatcgtttc cccggttctg ttttgaccga
     1501 ttaccgaata ctgaagaatt acgccaaaac cctgacagga gcaggagtat gaagtcagag
     1561 ataacaatca actaatactg tttcgttgat ttttgcttgt aattggcgtt ctggtctgat
     1621 ttttgtggag taagttgatg cgtgatattc agatggttct tgagcgttgg ggagcgtggg
     1681 cggctaataa tcatgaagat gtgacctggt cgtccattgc cgccggtttt aagggattaa
     1741 ttacttcaaa agtaaaatct cgcccgcaat gttgtgacga tgacgcgatg atcatttgcg
     1801 ggtgcatggc ccgtctgaaa aagaacaaca gcgatttgca cgatttatta gtagattatt
     1861 atgtagtcgg tatgacattc atgtcactgg caggtaagca ttgctgctct gatggttata
     1921 tcgggaaaag gttacagaag gctgagggca taattgaagg gatgttaatg gcattagata
     1981 tccggttaga gat
//
LOCUS       DAZFWL020000332         1991 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFWL020000332 DAZFWL020000000
VERSION     DAZFWL020000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.336"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(85..624)
                     /locus_tag="ACHKNP_RS26335"
                     /old_locus_tag="pgaptmp_005267"
                     /pseudo
     CDS             complement(85..624)
                     /locus_tag="ACHKNP_RS26335"
                     /old_locus_tag="pgaptmp_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
     gene            1855..>1991
                     /locus_tag="ACHKNP_RS26340"
                     /old_locus_tag="pgaptmp_005268"
     CDS             1855..>1991
                     /locus_tag="ACHKNP_RS26340"
                     /old_locus_tag="pgaptmp_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4707217.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SG"
BASE COUNT          631 a          480 c          350 g          530 t
ORIGIN      
        1 actttggttc ccactactta tttggtggac accactttgt ctaattcgtc agattctgac
       61 cagacggttc aggctgtacg cttacactga acgccattta ccacaaagct gattcttaat
      121 tgccgcagtc gtggcatcat taaaaatgaa tttatacctc acaggttctt ccgcactgtt
      181 tttctgaagt acttccagtt gtctgagggt ttctgactca tccgcgcctt tgcggagttc
      241 tttattcagt tttgacaaca atgctttttt ctgagccttt aaaagctctt ccttgttttt
      301 ccagacagcc agttcacttt tgtatctgga atataactgc atctcctgct ggtacagggg
      361 cttcattaat aatttgtcca ctgcggtttt tcgttctccg gactcggcaa gcgtcaggaa
      421 aaaaagagag accggccccc gcagtgtatt cagtctgcag acatcaatca gattctggca
      481 gacaagtgaa acagcgccca gggcagacgc tgccacaagc ccaaggggag cctgcgtaat
      541 ttgctgagct tcaataacgg catcacgaat gagtggaggc agcgaactca ggggaaactc
      601 atcacaaggc tgaccgaatg acatagtaac ctcactatac acagatataa aagcactgca
      661 attctggcta ttaatagcaa aaatagcagt caaatcaata tgaggtaaaa ttttttgttg
      721 atttaatggt taaataataa ccacggtgta ttttgctgct gaagaaagac aaaatgaatc
      781 tgtgtgtccc ctccttccct tcggagcaca tccactattc aaattttaca caaccacgta
      841 cgaacaaaaa gcatcatccc gggtattgca gataccggtt tcctgccggg aagaatcata
      901 aacagaacac cgggaataac gccttcagga atcacccccc ctcctgaaga agaataaaat
      961 aaagaagact acagttcctg cactgaatgc tgagtacaat attttatgga caggatgaca
     1021 tttttataaa taacaaaaac catttaatac tgatacatca ataaaatact tcttccctgc
     1081 tgacacagat aaaatgcacc attttttctt caaatacttc tgtataatcg ccagccaatg
     1141 atgagaatgg attaaaataa cacttcttcg acaaacacaa aaacagcctt cattttacat
     1201 catcatccag tttcagttaa ccccggacga gcgacagtgt ttcagtacag gaattaaaag
     1261 ccagcttttg ctcagtaaaa cacactcaac taataatatc tcagcagata ttatcctgac
     1321 caggacacct cacattactg aattctcttc cgacagtatc actgcacaaa taaaaaagaa
     1381 cgggataaat attcagaccg gaagaatggt gtcacaaaag catttaatca ttacgcatga
     1441 ccaggaaggg aaacacttaa ccacatggta tcaacagata tcattacgtg tgcactctta
     1501 taaaaaacac acagaagaag taagataatt ataaacgtaa atttaatatc tcccactgac
     1561 atctccccca caatacacta aaaaatactc acagcagtaa acacagacac acctgataat
     1621 catacagccg ataaatattt tcccctttac ggagaaatca gcctgctgtt taacaataac
     1681 ggcgtatttt catggaagga gatcacccag taacggataa gtcctgatct ccttcatgtt
     1741 aatacgcaac aatcccatcg attcaaaaca atttcagaca gatattatct ccgtgaatcc
     1801 cccgccacct ttccggtgcg cggggttttg tcttttttca ccgggaatac atgtatgaat
     1861 ccgtctgatg ccattgaggc aattgaaaaa ccgctctcct ccctgcctta ctcactttcc
     1921 cgtcacatcc tggaacatct gcgcaaactc accagtcacg aacccgtgat tggcattatg
     1981 ggtaaaagcg g
//
LOCUS       DAZFWL020000333         1987 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFWL020000333 DAZFWL020000000
VERSION     DAZFWL020000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.337"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(109..>1987)
                     /locus_tag="ACHKNP_RS26345"
                     /old_locus_tag="pgaptmp_005269"
     CDS             complement(109..>1987)
                     /locus_tag="ACHKNP_RS26345"
                     /old_locus_tag="pgaptmp_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4707218.1"
                     /translation="PDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTF
                     AITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWD
                     TPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQLAPGNYRLTVRAVNAWGQ
                     QGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQ
                     VETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGYLD
                     FFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSNEITQTVNKKLEDQSAAIQ
                     QIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAADRIAM
                     INPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADI
                     SGSVNANSGALNNVTINQNCTIKGMLEATQVRGDFVKAVSKAFPKKVGTWGNTETPNG
                     TVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIAS
                     RETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISI
                     R"
BASE COUNT          421 a          572 c          481 g          513 t
ORIGIN      
        1 atggctgcgg acaaaatggc ggcataaagt ttacgcataa ttacctctcg cttttctgca
       61 ataaaaaagg cgtcattcct gacgcccttt attggggtta taaatatttc aacgaatact
      121 gatgccggaa gcagcttttt tggtcacaat caccgtacag tcggtgatat tgcctgcccc
      181 ctgattgcct ttctggaaaa tcttaaactc cagagtgacg ctaccaccac cactaggcat
      241 atcaataact gcactgtaac taccgggaat ggccccttta gtttctctgg atgcgattaa
      301 tacgccgttt ttgcgaactt caaaaccata ccccgtgtat cgcgtgcctc ctgggttatt
      361 tccgctccct ggatcgtcat acgctatacc gttaaaaata atgggcggaa taataatctg
      421 gcggtcaaag ttatgatcat cgctgatggt gactgtaacc gtaccgtttg gtgtttccgt
      481 gttaccccac gtaccgactt ttttcgggaa ggcttttgat acagctttaa cgaaatcccc
      541 tctgacctgg gtcgcctcca gcatgccctt aatcgtacag ttctggttaa tcgtgacatt
      601 gttgagcgct cctgagttcg cattcacact gccactgata tccgcatttt tcgccgtcag
      661 tcgcccgtct ggtgtcaggg aaaatgccgg aggattaccg ccgctggtaa tggtgggagc
      721 cgtcagatat ttcaggaaca cttcattcat gaatatctga tcgccctgac caacaaacat
      781 cggctttgtg ttgccattcg caggattaat catcgcaatc ctgtctgctg ccagcagcac
      841 ctgactctgc atgccgtcgg gggtgttctc aataccggca ccgatacccg caatataaag
      901 gcgtccgtcc tgcatctgct gcagcttcac tgcccacatg ctgttcaggt tattatttgt
      961 atcaacctga accttctgta tctgctggat cgctgcactc tggtcttcca gtttcttatt
     1021 gacggtctgt gttatttcat tgctgacatc cgttatggac gtcctgattt cagtcaggtc
     1081 aggcgcaagc tgaccgttat caatctgcgt ccacagctcc tgagccagat gggttttccc
     1141 tatctcgcct ttgaaaaaat ccagatagcc tgatgcatca tcactcggct gaccgacagc
     1201 ctctacaaat gccgatttgc caacggtgtt cacactgcgg atataaaagt aataatcatg
     1261 gcccggtttg atattgatac tggcagctat ccagtacagc gccgtgccaa gatagcgggc
     1321 tgtggtttca acctgcctga tatcagcaat ccgcttttcc gagaaccaga actcaaactg
     1381 taccgtcgga tcataaaccg caagatgcgg cgtggcggtt atctgaaaat agcccggtgt
     1441 cagctcaatc cgagacggcg ctgccggtgc ggcaatccgg aacgataccg atgccggatc
     1501 gccctgctgt ccccacgcat ttaccgcccg gactgtcaac ctgtagttcc ccggcgccag
     1561 ttgcgtgaag cggtatgtgg tttctgtcgt cctggccgtg ctgaccagcc gctcactgcc
     1621 gtcatccgct gccacggtca ggcgaagcag gaagctcacg cccttcacca ccttcggcgt
     1681 gtcccagcgc gccagcacct gatattcccc gctgtctgcg gtgacttcgg cggtcaggtg
     1741 ctgcaccgct ggcggcgtga caccattcac cgtgccgctc tggtcgccgt caaagtgcgc
     1801 cccgttatcc acgatggctt ctttttccgg cacgtgctgc accgccgtga tggcaaaggt
     1861 gccgtccgtg ttttcccgga tggagacaca gcggaacagg cgacgacgca gtgacggcag
     1921 ggagagtccc catacaccgt atgtctccac accatcaggc agggtgctga cctgtatccg
     1981 gtccggc
//
LOCUS       DAZFWL020000334         1980 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFWL020000334 DAZFWL020000000
VERSION     DAZFWL020000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.338"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(27..740)
                     /locus_tag="ACHKNP_RS26350"
                     /old_locus_tag="pgaptmp_005270"
     CDS             complement(27..740)
                     /locus_tag="ACHKNP_RS26350"
                     /old_locus_tag="pgaptmp_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4707219.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     ATDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHM
                     SESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRHGYGQAIAEKCGYSSRSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(875..1072)
                     /locus_tag="ACHKNP_RS26355"
                     /old_locus_tag="pgaptmp_005271"
     CDS             complement(875..1072)
                     /locus_tag="ACHKNP_RS26355"
                     /old_locus_tag="pgaptmp_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4707220.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEVAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(1314..1844)
                     /locus_tag="ACHKNP_RS26360"
                     /old_locus_tag="pgaptmp_005272"
     CDS             complement(1314..1844)
                     /locus_tag="ACHKNP_RS26360"
                     /old_locus_tag="pgaptmp_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGU4707221.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            complement(1853..>1980)
                     /locus_tag="ACHKNP_RS26365"
                     /old_locus_tag="pgaptmp_005273"
     CDS             complement(1853..>1980)
                     /locus_tag="ACHKNP_RS26365"
                     /old_locus_tag="pgaptmp_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707222.1"
                     /translation="DALTHAEVLIDDEQFDEINIVRGQPVSGGRLGVKIYKIESE"
BASE COUNT          559 a          526 c          363 g          532 t
ORIGIN      
        1 gccaaaaaat aataatcaga tgaaaatcaa taatcaagcc cttgcctgga tacatatctg
       61 tctggcggga agccataata gcggtgaaat acagaaataa agtaggacct gcttgaataa
      121 ccgcattttt ctgctatagc ctgtccatat ccatgccggg aacataacat atttacagca
      181 acacgcatcc gctcttccag caacaagcga ctgaacatgc ccccttcatt tttcagtttt
      241 gtctttaaca aactctcact catatgcaac tgtagagcaa tcgcaccaag cgtccagctt
      301 gctgatatat ctgtctgaat tatcgccctg actttggcac ttatgctgga taaacatcca
      361 cttaaaaaca atgacatccg ttcatctgtt tcaaacagag acaggcaggc catcataaga
      421 aacatatccg tggcctctcc ggaaaatccc tggctggtaa ttaaagccgc agccaacgca
      481 ggattgttgg gttccagcaa caggtaaagc ggaatgtcag tcagacgagt cctcgtcagc
      541 ttatgctgac tttccagata ttgacttacg acggattcgc ttatatcgac aattttaact
      601 ttgccataat gcataaggaa aagctccctg atgcatttgg tggccagaac gactgagcct
      661 ggcttaagtg acaacgtatc cttttcaaga aaaatattaa ttggggagca aaccatgata
      721 actgaacaga caacagccat tataatttta ctttcattag caattggtta gctcaattat
      781 agccccaaaa ggtaaattat catcaacaca taagcaaagg actgacaggt gtcgcccccc
      841 caccagccgc ccattcacca caaataaaaa gccttcagga ctgaaggcgt ctgtaacaac
      901 cgcactgata gtctgccaga cccgccataa caagctgggt cagtattaac tggcagcgtt
      961 cgcgtgaaag gtaagtattc tgcgcaacct ccccgactgt cgccggttcg gtaacgctta
     1021 attcattaaa caccactctg gcggtttcgg tcatatcctg ctgtttcagc atgtcttttt
     1081 ccctttttcg gttaacgtga cacaccaata actcttgtcg aaaaagccag caagctgaaa
     1141 gaacggtatt aataaccacc agcgaattta ttgcgctgct gtatattacg gacacaaaaa
     1201 aaccaccttc cggtggcttc cttgtgcgaa aaaacttgca tttcgcctcg cgatacagct
     1261 ttgcgaagct tacaggaatt caagctgttt ctgcgtaaaa aagcaagctt tttttatcga
     1321 aatgaatcgt gcataggtac ataaagcatg tgttcagcca cggctaacca acctgcaata
     1381 cgtttctcac atgtgctgaa acaccattcc gggtgagtac gatttaaaca ttctgccatt
     1441 tttctcttac tcattccccg tccttcgtac ctttgccgga gaatattgat tagcccggga
     1501 tattccccaa gcacctcact gataacgcga tcaataatca acgcctctgt gtctgtacaa
     1561 tgtgacaacc agctcttctg cttccctctg gtcatatccc gaaaaaatgc ctcaagttcc
     1621 ggtttttcca gcccggattt cttcatgctg cgtaaaacct cattaactgc tgttttcgtc
     1681 agctttttcg aaaccagtaa ccggttaaac atatttccgg atttaccccc accgatatac
     1741 gaccaccgcc cccacatccg taatttcccc tggatccaga ctgcttccag cgtgttcagg
     1801 cgtaaatgtt cgccgctttt gcctgtaatt tccggatata tcatatttac gctcactcac
     1861 tctcaatttt gtaaatcttc acacccagcc gtccaccaga tactggctga ccgcgcacaa
     1921 tattgatttc atcaaactgc tcgtcatcaa tgagcacttc cgcatgcgtc agcgcatcca
//
LOCUS       DAZFWL020000335         1961 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFWL020000335 DAZFWL020000000
VERSION     DAZFWL020000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.339"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1174
                     /gene="escC"
                     /locus_tag="ACHKNP_RS26370"
                     /old_locus_tag="pgaptmp_005274"
     CDS             <1..1174
                     /gene="escC"
                     /locus_tag="ACHKNP_RS26370"
                     /old_locus_tag="pgaptmp_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HGU4707223.1"
                     /translation="KYLRDTISVNKNSCNVRKITTFNSIEVRGVPECIKYITSLSESL
                     DKEAQSKAKNKDVVRVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNGSLPSTG
                     KGAVERNGNLFDNSVKISADPRLNAVVVKDREITMDIYRQLISELDIEQRQIEISVSI
                     IDVDANDLQQLGVNWSGTLNAGQGSIAFNSSTAQANLSSSVISNANNFMIRVNALQQN
                     SKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTPRILDDN
                     SNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSILPEVQNSEMTTEATLSAGESLL
                     LGGFIQDKESSSKDGIPLLSDIPVIGNLFSSTTKQKHSVVRLFLIKATPIKSASSE"
     gene            1188..1643
                     /gene="cesD"
                     /locus_tag="ACHKNP_RS26375"
                     /old_locus_tag="pgaptmp_005275"
     CDS             1188..1643
                     /gene="cesD"
                     /locus_tag="ACHKNP_RS26375"
                     /old_locus_tag="pgaptmp_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HGU4707224.1"
                     /translation="MSRKFSSLQDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNRKMAKKAFKACLMWCNEQEKYATYKENIKKLLGNTD"
BASE COUNT          650 a          375 c          384 g          552 t
ORIGIN      
        1 aaaatatctg cgtgatacaa tctcagtaaa taaaaactcc tgtaacgtga gaaaaatcac
       61 aacatttaac tcgattgaag ttcgtggcgt tcctgaatgc ataaaataca ttacatcttt
      121 atctgaaagt cttgataaag aagcacagtc taaagcaaaa aataaagatg tcgttagagt
      181 atttaaatta aactatgcat cagcgacaga tataacgtat aaataccgcg accaaaatgt
      241 tgtcgtgcct ggcgtcgtct ccatcctgaa gacgatggca agtaatggct cacttcccag
      301 cacaggcaaa ggtgctgtag aaagaaacgg caacctgttt gataacagtg taaagatttc
      361 cgcagatcca cgtcttaatg cagttgttgt aaaagatcgt gaaataacta tggacatata
      421 tcggcaatta atttctgagc ttgatattga gcaaaggcaa attgagattt ctgtttcaat
      481 tattgatgtt gatgcaaatg atcttcagca actgggagta aactggtccg gtacattaaa
      541 tgcagggcaa ggttctattg cctttaacag ctcaacggcg caagctaatc tcagttcttc
      601 tgtgataagc aacgccaata atttcatgat cagagtgaat gcgttacaac agaactcgaa
      661 agcaaaaata ctctctcagc catcaattat aaccttaaat aatatgcagg cgattcttga
      721 taaaaacgta acgttttata caaaagtttc cggagaaaag gtggcatctc tggaaagtat
      781 taccagcgga acattactga gagtaacacc acgtatactt gatgacaact cgaatagtct
      841 taccggaaaa cgtcgggaac gagttcgttt gttacttgat attcaggatg gcaatcaatc
      901 gactaaccaa agtaatgctc aggatgccag cagcatctta cctgaagtac aaaattcaga
      961 aatgacaaca gaagcgacat taagcgctgg agaaagtctg ctgcttggcg gatttatcca
     1021 ggataaagag agttcaagta aagatggtat accgttgtta agtgatattc ctgttattgg
     1081 taacttattc tccagtacaa caaaacaaaa acacagcgtt gttcgactct ttttgattaa
     1141 agcgaccccg ataaaatcag catctagcga ataataaatg aggctttatg agcaggaaat
     1201 ttagctctct acaggatatc tatgatttct accaggatgg tggtacgtta gcatcactga
     1261 caaatttgac gcaacaagat ctcaatgatc ttcattctta tgcctataca gcatatcaat
     1321 ctggtgatgt tataaccgca agaaatctat ttcatctgct aacatatctg gagcactgga
     1381 attatgacta caccctatct ctggggttgt gtcatcagcg tttatcaaat catgaagatg
     1441 cacaactgtg tttcgctcgc tgtgcaactt tagttatgca agatcccagg gcatcttatt
     1501 attctgggat tagctactta ttggtcggaa atcggaaaat ggccaaaaaa gcctttaagg
     1561 cttgtttaat gtggtgtaat gaacaagaaa aatacgctac atataaagaa aatattaaaa
     1621 aattgttagg taatacagat taaaaactac catctgccat accaatcccc aggctgtctg
     1681 gggattttct atcggaaata ataaagctgc tttggtgaag tgcgcccaac gttgagcacc
     1741 gaccgctggc agatttatct acaggagtgg gttactccat taaccaggat tttatgcgcc
     1801 tgaaccatct tctgctgtac tcaatgtcat ttccgcttat agaataagca gtatatatct
     1861 taacttagca tcagaaatct gactgtttca atcatctata gcccagagtg tatttgtcag
     1921 gaaatgactt tcaaactttg gcagagaaaa aggcttatcc t
//
LOCUS       DAZFWL020000336         1960 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFWL020000336 DAZFWL020000000
VERSION     DAZFWL020000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.340"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            196..1023
                     /gene="cif"
                     /locus_tag="ACHKNP_RS26380"
                     /old_locus_tag="pgaptmp_005276"
     CDS             196..1023
                     /gene="cif"
                     /locus_tag="ACHKNP_RS26380"
                     /old_locus_tag="pgaptmp_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HGU4707225.1"
                     /translation="MKDITLPPPTSASCLTGAISVNTEAVLSPMQHTSALHVRDFASL
                     CSQNLKANVLLNSDDHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREK
                     GIVEPSCGVTANAIMKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVND
                     GALGHAYIVDIPKGENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNY
                     FSCMSQNKTDLEQIATLFDIDGNVKMLRKENIQYQKHDNFSFQLFEYDTDNIEKTLR"
     gene            complement(1159..1679)
                     /locus_tag="ACHKNP_RS26385"
                     /old_locus_tag="pgaptmp_005277"
                     /pseudo
     CDS             complement(1159..1679)
                     /locus_tag="ACHKNP_RS26385"
                     /old_locus_tag="pgaptmp_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1723..1803
                     /locus_tag="ACHKNP_RS26390"
                     /old_locus_tag="pgaptmp_005278"
                     /pseudo
     CDS             1723..1803
                     /locus_tag="ACHKNP_RS26390"
                     /old_locus_tag="pgaptmp_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          587 a          370 c          432 g          571 t
ORIGIN      
        1 gcatggatgg aggtggtaaa aagtggtgtg gctggcaatc caggccgcgt cacagaaatg
       61 gacaatgccg ctgagagact ggcgaatggc atacaggcac cagaaaaatt tcagacatga
      121 cataatgccc atgccagaag ttctttttgc acctcgcctg tatttataga taaatataga
      181 gtgaatgaat gagatatgaa agacattacc cttccccccc cgacgtccgc gtcctgtctg
      241 acaggggcca tatctgtaaa tactgaagct gtattatctc ccatgcaaca cacttcagcc
      301 ttacatgtaa gagattttgc ttccctgtgc tcacagaacc tcaaagctaa tgtattgcta
      361 aatagtgatg accacgaagt acctatacat cagaaaaatc ctgctgcaat aatgcaaaat
      421 atcgactcta acatcaaaca gatggcaaca gactggggga tgtcgattga ggaggttgag
      481 gttattatag ggcgagagaa aggcattgtg gaaccctcct gcggagttac cgctaatgct
      541 attatgaaac tatttctgga caaggatggc ttcagttact gctttgaaaa tgaacagaca
      601 ctatcgctcg agcagcttca ggagcgcctg tcctgtatgc ctgaatgtaa gagctttgta
      661 ttacgtgtta atgatggtgc gcttggtcat gcttacattg tcgatattcc caaaggagaa
      721 aactcttgtc gtcctgcatt cttgtatcag tcagatttag gagagggcgt caccagaaag
      781 ttaagatttg aggactggat gacgcataaa gcattgactc cgattttgct ggatgatatt
      841 tgtaattact tctcctgcat gtctcaaaat aagacagatt tggagcagat tgcaacgtta
      901 tttgatattg atggaaatgt taaaatgtta cgaaaggaaa atattcaata tcaaaagcat
      961 gacaatttta gtttccagtt gtttgagtat gacaccgata atattgaaaa aacattgaga
     1021 taataaaatc actatgtagt tagcttttgt taaaactgca attatttgta aacataataa
     1081 taagtattat gacgttaaaa tttaaccata cggtaaaatg attcaggaaa accggttcag
     1141 catagcctag gctgaacttt tgaggatatc aacgaacgga atttttgaac atttatgagt
     1201 agatcgttgt tgaagggctt caatgagcat gtcaagttca tcaactgctg gtctacgatt
     1261 acgacggttt gatttaccaa tcaaagcaag tttaagtaga tatggacgag cacttttcgc
     1321 cgggtttgat gtgtaattaa ttccgtatat aggtttggct gcatccagaa cactgccaag
     1381 ataactaaca tcgtgactga ctgttgctgg acctgcacca gcgttgtttc tcagcctgca
     1441 atgttcaatt acgtcatttt ctgtcagttc agatagtttg atcgcggaga tgtcactatc
     1501 cataagcagt tctagcacat atcttttagt acggtctgct ttacctccgg catttggtca
     1561 tttaaatatt tgtgtagtaa gtcacggact gtaagtccgt caacagcatt tgatgatgaa
     1621 atgccatata gatctaatac catcactttc tgtgtgcccc atgttttagc atgagcatga
     1681 acgcggcggc tatctcacgg gtgatcatcc ctttggcata ctgaaaagta tcgggtgtta
     1741 ctgaaccgtg ttgatacatc aactgcactg gatattgagt ggccgataat accgacgtta
     1801 taacctataa atgccaatca ggctgaatgt tgaaaggata gaatgaacag cccgacacac
     1861 aaaaaacgga gtccggctcc ggtttttgtg ttgccatata aggcagatgt ttgttacagc
     1921 tatttaagtc tggagttcaa attaaaatag ggagttttgt
//
LOCUS       DAZFWL020000337         1958 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFWL020000337 DAZFWL020000000
VERSION     DAZFWL020000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.341"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..941
                     /gene="espB"
                     /locus_tag="ACHKNP_RS26395"
                     /old_locus_tag="pgaptmp_005279"
     CDS             <1..941
                     /gene="espB"
                     /locus_tag="ACHKNP_RS26395"
                     /old_locus_tag="pgaptmp_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HGU4707226.1"
                     /translation="TNQVMTVNSVSENTTGSNAITASAINSSLLTDGKVDVSKLMLEI
                     QKLLGKMVRILQDYQQQQLSQSYQIQLAVFESQNKAIDEKKAAATAALVGGAISSVLG
                     ILGSFAAINSATKGASDIAQKTASTSSKAIDAASDAASDTATKTLTKATESVADAVED
                     ASSVMQQAMTTATRAASRTSDVADDIADSAQRASQLAENAADAAQKASRASRFMAAVD
                     KITGSTPFIAVTSLAEGTKTLPTTVSESVKSNHEISEQRYKSVENFQQGNLDLYKQEV
                     RRAQDDIASRLRDMTTAARDLTDLQNRMGQSVRLAG"
     gene            948..1355
                     /gene="cesD2"
                     /locus_tag="ACHKNP_RS26400"
                     /old_locus_tag="pgaptmp_005280"
     CDS             948..1355
                     /gene="cesD2"
                     /locus_tag="ACHKNP_RS26400"
                     /old_locus_tag="pgaptmp_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HGU4707227.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETSFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            1392..1613
                     /gene="escF"
                     /locus_tag="ACHKNP_RS26405"
                     /old_locus_tag="pgaptmp_005281"
     CDS             1392..1613
                     /gene="escF"
                     /locus_tag="ACHKNP_RS26405"
                     /old_locus_tag="pgaptmp_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HGU4707228.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            1619..1897
                     /locus_tag="ACHKNP_RS26410"
                     /old_locus_tag="pgaptmp_005282"
     CDS             1619..1897
                     /locus_tag="ACHKNP_RS26410"
                     /old_locus_tag="pgaptmp_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscG/YscG/SsaH family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HGU4707229.1"
                     /translation="MVNDVSANKILVWAAVAAANHKLPKYAESILNVLPQIIPDKKDI
                     AHLEFIILYGLNRKNDAVKALEDFMDDETSQLLCSLVHENNRSWTLMF"
BASE COUNT          584 a          367 c          448 g          559 t
ORIGIN      
        1 atactaatca agtaatgacg gttaattctg tttcggagaa tactaccggc tctaatgcaa
       61 ttaccgcatc tgctattaat tcatctttgc ttaccgatgg taaggtcgat gtttctaaac
      121 tgatgctgga aattcaaaaa ctcctgggca agatggtgcg tatattgcag gattaccaac
      181 agcaacagtt gtcgcagagc tatcagatcc aactggccgt ttttgagagc cagaataaag
      241 ccattgatga aaaaaaggcc gctgcaacag ccgctctggt tggtggggct atttcatcag
      301 tattggggat cttaggctct tttgcagcaa ttaacagtgc tacgaaaggc gcgagtgata
      361 ttgctcaaaa aaccgcctct acatcttcta aggctattga tgcggcttct gatgcggctt
      421 ctgatactgc gactaaaacg ttgactaagg caacggaaag cgttgctgat gctgttgaag
      481 atgcatccag cgtgatgcag caagcgatga ctacagcaac gagagcggcc agccgtacat
      541 ccgacgttgc tgatgacatt gccgattctg ctcagagagc ttctcagctg gctgaaaacg
      601 ctgcagatgc cgctcagaag gcaagtcggg caagccgctt tatggctgca gtagataaga
      661 ttactggctc tacaccattt attgccgtta ccagtcttgc cgaaggcacg aagacattgc
      721 caacaacggt atctgaatca gtcaaatcta accatgagat tagcgaacag cgttataagt
      781 ctgtggagaa cttccagcag ggtaatttgg atctgtataa gcaagaagtt cgcagagcgc
      841 aggatgatat cgctagccgt ctgcgtgata tgacaacagc cgctcgcgat ctcactgatc
      901 ttcagaatcg tatgggtcaa tcggttcgct tagctgggta attgatcatg gtcgatacgt
      961 ttaatgatga agtgcttaat cactatcttg aacaaaaagg gtacacaata cagaaggagt
     1021 ttctttgtgg cagtgccttt tttatcggat ggcggattga gacctctttt ttctcattag
     1081 cgtacagact tgatgaacaa gaactgattt tgtgctcttt tgaagcacgt aaccaaacag
     1141 ggcttaacgg ccctgtttta tcactgactc gcttgctcga agagttgtac caccattttt
     1201 cgggtattaa gaaaatcagt gcgatgaaat ctaagattgg ttcagattca gaacgtcaaa
     1261 agcgcgaaga gttgtttaat tacttcatca gaaagggcgc tgtgcagcaa gaaacagaag
     1321 acggaatttg gttcgtaatg aatgtaaata gttagaaatt tttatttcta ttaactattg
     1381 aggaaaattt aatgaattta tctgaaatta ctcaacaaat gggtgaagta ggtaaaacgc
     1441 tgagtgattc tgtgccagag ttacttaata gcaccgattt ggttaatgac cctgaaaaaa
     1501 tgttagagct gcagttcgcg gttcagcaat attctgctta tgttaacgta gaaagtggaa
     1561 tgctgaaaac gataaaagat ttggtctcaa ccatttccaa ccgtagcttt taatttttat
     1621 ggttaatgat gtttctgcca acaagatact ggtgtgggcg gctgtagctg cggcaaatca
     1681 taagcttccc aaatatgcag aatctatcct gaatgtattg ccgcaaatta tacccgataa
     1741 aaaagatatc gcacatttag aatttattat tttatatgga ttaaatagaa aaaatgatgc
     1801 ggtaaaggct ctggaggact ttatggatga tgagacaagc cagttgttat gcagtctggt
     1861 ccacgagaat aatagaagct ggacactaat gttttaatct catattgagt ttaaacaatt
     1921 gataaatttc attttggtat gctataaatt ttaacaga
//
LOCUS       DAZFWL020000338         1943 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFWL020000338 DAZFWL020000000
VERSION     DAZFWL020000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.342"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1234..1836
                     /gene="fimB"
                     /locus_tag="ACHKNP_RS26415"
                     /old_locus_tag="pgaptmp_005283"
     CDS             1234..1836
                     /gene="fimB"
                     /locus_tag="ACHKNP_RS26415"
                     /old_locus_tag="pgaptmp_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HGU4707230.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          593 a          345 c          401 g          604 t
ORIGIN      
        1 gtttaattga tttgaatgtt cgtaagctat atcacttact caatccattt tacccagagt
       61 cataaaatag agtgtctgtt gatctcattt tcatattcct ttcagactga agtgcatttt
      121 catcacccga tgtgaaagaa ctgaagtgat gtttgccata gattgcgaga tgccctgtct
      181 cattgactat cttggtgcac ctttatacct gttataccag atcaaaaatc acgcaatcca
      241 tacaacaaaa ccagatttgc aattcgtgtc acaaaatatg tcgatatttt tctaagaaga
      301 agatgccatg tgaagccaaa cgaacacttg cggaggcctt cacaaaccaa gggttgttat
      361 ttaattatct gttttataag taatttattt agagtataaa caaggaaaga tttgcaagat
      421 aactcagtgg gcgtggtgaa aataagtgtt tacccgccat caggctgagc ataattctca
      481 tcatgaaata tgtttcctgg tttgtggctt gtagctggtc actcctgaag tctctctgga
      541 gaggcttgtt gatgttggtg ttttcaggat gatgtttcac ttagcttgtt tgccgaatag
      601 cccggcgaac agctgtgatt gaggaaggtt taagtcgcag tgaccaaagc catatttacc
      661 aacggatgca gatgaaaaaa tcatctcctg ctttccccca tatctccagg ataaaaagga
      721 atgtaacaat ctcatgaact aaactgacga accagccagg aataatcgct agggacctaa
      781 gaattagcct gataatagct actaagaaat tactgcgttc catgaaatag ccattttgtg
      841 gcaaatggag ttgtctaata atgtcatatg tgagacggat agttgaacga atattaaatt
      901 ttgctgaatt ttttatgttg attttacttg ttacagaaca tatcacatga tatatagata
      961 agattagttg cattaatgat gagggttatt attagattcg tatccgattg ataaatatat
     1021 aaaggtacat agcatgcaag agcatagcgt ttgtatggca acgttattat aagtaactgt
     1081 tgctactcca tttaagttca ctcagaagaa ctggtccact tacgttagtt attaagcaaa
     1141 cgttcgcttt tataaacata atcaggataa aaatgttgga ttattgctaa cccagcacag
     1201 ctagtgcgcg tctgtaatta taagggaaaa acgatgaaga ataaggctga taacaaaaaa
     1261 aggaacttcc tgacccatag tgaaatcgaa tcactcctta aagcagcaaa taccgggcct
     1321 catgcagcac gtaattattg tctgactttg ctttgtttta ttcatggttt ccgggcgagt
     1381 gaaatttgtc gattgaggat ttcggatatt gatcttaagg caaagtgtat atatatccat
     1441 cgattaaaaa aaggcttttc aacaacgcac ccgctattga acaaagaagt tcaggcttta
     1501 aaaaactggt tgagtatccg tacttcgtac ccgcatgctg agagcgaatg ggtattttta
     1561 tcgcgtaagg gaaatccgct ttctcggcaa cagttttacc atattatctc gacttccggt
     1621 ggtaatgccg ggttgtcact ggagattcat ccgcacatgt tacgccattc gtgtggtttt
     1681 gctttggcga atatgggaat agatacgcga cttatccagg attatcttgg gcatcgcaat
     1741 attcgtcata ctgtctggta taccgccagc aatgcagggc gtttttacgg catctgggat
     1801 agagccagag gacgacagcg tcacgctgtt ttatagagat agttgagata ccagggatgg
     1861 tgttaaaaac tacattattc ttacgatata tcaaaaatga ttaaccattt attaattgat
     1921 gttattgtaa gtgtgattat taa
//
LOCUS       DAZFWL020000339         1928 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFWL020000339 DAZFWL020000000
VERSION     DAZFWL020000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.344"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..552
                     /locus_tag="ACHKNP_RS26420"
                     /old_locus_tag="pgaptmp_005284"
     CDS             <1..552
                     /locus_tag="ACHKNP_RS26420"
                     /old_locus_tag="pgaptmp_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4707231.1"
                     /translation="TWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAE
                     DLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFV
                     LATPTETDGALFPGRIMLANTCMWDYRSDECGYNGPAVADEFDNPTTDIRKDRCSKCM
                     RGCELRRNVGNFGGFLSINKLSQ"
     gene            563..1306
                     /locus_tag="ACHKNP_RS26425"
                     /old_locus_tag="pgaptmp_005285"
     CDS             563..1306
                     /locus_tag="ACHKNP_RS26425"
                     /old_locus_tag="pgaptmp_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4707232.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVSRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMEGT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            1303..1884
                     /locus_tag="ACHKNP_RS26430"
                     /old_locus_tag="pgaptmp_005286"
     CDS             1303..1884
                     /locus_tag="ACHKNP_RS26430"
                     /old_locus_tag="pgaptmp_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4707233.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMAAWGAALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKPKTPEYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQA"
BASE COUNT          388 a          499 c          644 g          397 t
ORIGIN      
        1 acctggcagg ggcgtgaata tcaggcgtac ccgattgagg ggagtggctt tgagatgaac
       61 ggaaagggca gcagtgcccg cccgtcgctg acagtgtcca atcttttcgg ccttgtcacc
      121 gggatggcgg aggatttgca gagcctggtg ggtgccacgg tggtccgtcg ccgggtgtat
      181 gcgcgttttc tggatgcggt gaattttgtg gcaggcaatc ctgaggcaga cccggagcag
      241 gagctgacgg accggtgggt ggtggagcag atgtcatcgc tgacggccat gacggcctcg
      301 tttgtgctgg cgacaccgac ggagacggac ggtgcgctgt ttcccggtcg catcatgctg
      361 gcgaacacct gtatgtggga ttaccgctct gatgaatgcg ggtataacgg tccggcagtg
      421 gcggatgagt tcgacaaccc caccacggat atccggaagg acagatgcag taaatgcatg
      481 cgcgggtgtg aactgcgcag gaatgtcggc aactttggcg gcttcctttc cattaataaa
      541 ctttcgcagt aaatcccggt ttatgacaca gactgaatca gcgattctgg cgcatgcccg
      601 gcggtgtgcg cctgcggagt cgtgcggctt cgtgataagc acgccggagg gggagtggta
      661 tatcccttgt gtgaatattt ccgcagagcc ggaggcgtat tttcgtatcg caccggaaga
      721 ctggctgcag gcagagatgc agggggagat tgtggcgctg gttcacagtc atcccggtgg
      781 tctgccctgg ctgagcgagg ctgaccggcg gctgcagata aaaagcgcac tgccctggtg
      841 gctggtcagc cggggtgaca ttcacaaatt ccgctgcgtt ccgcacctga ccggacggcg
      901 ctttgaacac ggggtgacgg actgttacac cctgttccgg gatgcgtacc atctggcggg
      961 gactgaaatg ccggattttc atcgcgagga tgactggtgg cgcaacggcc agaacctgta
     1021 cctggacaat atggagggaa cgggctttta ccgggtgccc ctgtcctctg cacaggcggg
     1081 cgatattctg ctgtgctgct ttggtgcttc ggtaccgaac catgccgcca tttactgtgg
     1141 caacggtgag ctgcttcacc atctgcctga acaactgagt aaacgggaga ggtattccga
     1201 aaaatggcaa cgacgaacgc attctgtctg gcgtcaccgc cactggcacg catctgcctt
     1261 cacggggatt tacaacgatt tggccgccgc ctcagcctgt atgtgaacac ggcagcggaa
     1321 gccatccggg cgctgtcgtt acaggtgccc ggattccgcc gtcagatgaa cgaaggctgg
     1381 taccagatac gtattgccgg tgatgacacg gcaccggagg cggtgtacgc ccgtcttcac
     1441 gaacagctgg gtgagggaac ggtcatccac attgtgccgc gactggccgg ggccggaaag
     1501 ggtggactgc agattgtgct gggggcggca gccatcgtgg gctctttctt cacggccgga
     1561 gcatcgatgg cagcctgggg tgcagcgctg agtgccggcg gtttttctgc caccacgatg
     1621 ctgttttcac tgggtgccag catgatactg ggtggtgtgg cacagatgct ggccccgaag
     1681 ccaaaaacac cggaatacag ggcaacggat aacggtaaac agaacacgta cttttcgtcg
     1741 ctggacaaca tgattgccca gggtaacccg atgccggtgc cttacgggga aatgctggtt
     1801 ggctcccgcc gtatatccca ggacatcagt acccgtgatg aaggcggggg cggaaaggtc
     1861 gtggttatcg ggcggcaggc ataaaagcga aaaaatcccg cagtgctcac ggacaggaac
     1921 tgcgggag
//
LOCUS       DAZFWL020000340         1924 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFWL020000340 DAZFWL020000000
VERSION     DAZFWL020000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.345"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(74..373)
                     /locus_tag="ACHKNP_RS26435"
                     /old_locus_tag="pgaptmp_005287"
     CDS             complement(74..373)
                     /locus_tag="ACHKNP_RS26435"
                     /old_locus_tag="pgaptmp_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4707234.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            complement(379..612)
                     /locus_tag="ACHKNP_RS26440"
                     /old_locus_tag="pgaptmp_005288"
     CDS             complement(379..612)
                     /locus_tag="ACHKNP_RS26440"
                     /old_locus_tag="pgaptmp_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707235.1"
                     /translation="MRDIYHETIDRAFSALAYAEGMYEILRIWLETLGDNERDKQKSR
                     IVTALITLLEPVINELQEIETLHDRYNEQHTGE"
     gene            complement(614..835)
                     /locus_tag="ACHKNP_RS26445"
                     /old_locus_tag="pgaptmp_005289"
     CDS             complement(614..835)
                     /locus_tag="ACHKNP_RS26445"
                     /old_locus_tag="pgaptmp_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707236.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSISEIMRVAFFFLCTDEHR
                     DTGLNILDIAANYADFVTEAVLRKTTDGD"
     gene            complement(828..1049)
                     /locus_tag="ACHKNP_RS26450"
                     /old_locus_tag="pgaptmp_005290"
     CDS             complement(828..1049)
                     /locus_tag="ACHKNP_RS26450"
                     /old_locus_tag="pgaptmp_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707237.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(1039..1230)
                     /locus_tag="ACHKNP_RS26455"
                     /old_locus_tag="pgaptmp_005291"
     CDS             complement(1039..1230)
                     /locus_tag="ACHKNP_RS26455"
                     /old_locus_tag="pgaptmp_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4707238.1"
                     /translation="MATIPTLVHSQTAFLWRFIIFGASEHQIIHVTAWTEREARSRCP
                     SGCVAVFAARIRQEVCHVQ"
     gene            complement(1261..1701)
                     /locus_tag="ACHKNP_RS26460"
                     /old_locus_tag="pgaptmp_005292"
                     /pseudo
     CDS             complement(1261..1701)
                     /locus_tag="ACHKNP_RS26460"
                     /old_locus_tag="pgaptmp_005292"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            1730..1903
                     /locus_tag="ACHKNP_RS26465"
                     /old_locus_tag="pgaptmp_005293"
     CDS             1730..1903
                     /locus_tag="ACHKNP_RS26465"
                     /old_locus_tag="pgaptmp_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707239.1"
                     /translation="MSTPLEKRSPPYQMRLPEDFRAQLEEAAKADGDTSLATWIKRVL
                     RKELQQRGIEPKG"
BASE COUNT          482 a          469 c          465 g          508 t
ORIGIN      
        1 gcatcactac ccgcaaaaat aatgacttcg gtcatcttgt cgtaaggctg tttttttaag
       61 ttcggtgcgc ttttcatttc ttgcccctga atccgttaat catggttttc agcttctgga
      121 tgtttgcccg tgccctggcg ttgctggtgg gcacgttatg cggcgcggtc tgtaccagag
      181 aaaaatcacg ccggaactga taaacaggca tcacgcaatc atattcgtaa ccttcacggc
      241 ggtaagttac gcaccgtccc gccacgccct taatcattac cgtgccgccg tactggtcgc
      301 ggtaaatatc accgcgcgta aatttagggt gagtgttgcc actggcagtt aagccagaat
      361 atttaagttt cattattttt attctccggt gtgctgttcg ttatatctgt cgtgcaatgt
      421 ctctatttcc tgcaactcat ttattacagg ctcaagaagc gttattaatg ccgtgacaat
      481 tcttgatttt tgtttgtcgc gttcattgtc gccaagtgtt tcaagccata tgcgcaatat
      541 ttcgtacata ccttcagcgt aagcaagggc actaaatgcg cggtctattg tttcgtggta
      601 aatatcacgc atattaatcc ccgtccgttg tttttcttaa aacagcttct gtcacgaaat
      661 cagcatagtt agcggcaata tcaagaatat ttagccctgt atctctgtgc tcatcagtgc
      721 aaagaaagaa aaaagccacc cgcataattt cagatattga cgaaagcgca tcagccgcat
      781 catcaggaac gccggaaaat tcttgtttca gggaattaaa acgatcatca cgcataaccc
      841 cccccatttt cacaatcagc aacaagaata tttttagcgt cattcagcga ccgcgttgcg
      901 atgtatcgga tacattccag ggcgaactgt gtccggtctt tctgttcttc tccctgcgca
      961 agctctgcag tgcattcaat atcgataagc gcgtgcatca gcgtagtgag cgcggcggct
     1021 gctgcgtccg gtgtggtttt attgcacatg gcacacctcc tgacgaatac gggcggcgaa
     1081 tacagcaaca caaccggacg ggcaacggct acgcgcttcg cgttccgtcc aggcggttac
     1141 gtggatgatt tgatgttctg acgcgccaaa gatgataaag cgccagagaa aggcggtttg
     1201 agaatgtaca agggtaggga tagtagccat gttggcagcc tcctcagtgt ggttaagata
     1261 accgccaccg gaaacgccaa tttcgctggt ggcggactgt acagggttgg cgtaaccgga
     1321 cactgaagaa accggcgcgg attgctccgc ccccgtacag cccaccattg ataaggtgtg
     1381 actatgcggc acaaaaaaac acgcggggcg cgtgttatgc gcttcagttt tgcccaggac
     1441 gccaatcccg gcacccgttt tatgaggtgc gcgggaatca tagctatttt cgacactggc
     1501 gatcaactgg tttttttctc cgatattcag ggccagaaaa cgccacacaa aggccgtttg
     1561 tgtgtgtaca aggtgtggta tatgatttac ggcaaccata acggctccta gtttacgttg
     1621 ttggttagac gccccgtatg tgttcccagc actgcggggc gttgattttt gtgtaatggc
     1681 aagtacaatg gtaattacca tgtaggtata gtaatgaagg gtaattaccg tgtcaacacc
     1741 attagaaaaa cgttcgcctc cataccaaat gcgattaccg gaagacttca gagcgcagct
     1801 agaagaagca gctaaggccg atggcgacac atctttagcc acttggatta aacgcgttct
     1861 ccgcaaagaa ttacagcaac gcggcataga acccaaagga taaaaactat cagcgccgtg
     1921 gtgt
//
LOCUS       DAZFWL020000341         1923 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFWL020000341 DAZFWL020000000
VERSION     DAZFWL020000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.346"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            58..1491
                     /locus_tag="ACHKNP_RS26470"
                     /old_locus_tag="pgaptmp_005294"
     CDS             58..1491
                     /locus_tag="ACHKNP_RS26470"
                     /old_locus_tag="pgaptmp_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HGU4707240.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEERINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
BASE COUNT          578 a          336 c          447 g          562 t
ORIGIN      
        1 tattgtagcg ggatttgctg ctattttact aacccattaa tgaaaagaga ggcttttatg
       61 tgcttacttg caccagaaaa tccttatccg atatatgcac taccaccgct ggtgagaaat
      121 gcaataattg aaactcaaaa aaatacacag gcacctttgg ctatggtggc aacatccgca
      181 ttaacagcga tctcaattgc ctgtcagaat cagattgacg tgtgcagacc tggaaattta
      241 catgggcctg ttaatcttta ctctctgatt ctggctgatt ccggtgaaag gaaaacgacc
      301 gtggataaag tgtttatgaa agcattttat ctcagggatg aagccctggc ggatgaatac
      361 gcgaaactgg ttgagaatta cagtacagaa aaggaaatat gggagcaaaa acaaaaagcg
      421 ctggaatcaa aatttcataa agagattcgt gccggtaaag attacaaggc aacagaatca
      481 gagcttgaaa cgcatctgaa taaacctcct gttccgccgc agatacgtcg aacaattttt
      541 aatgagacaa cgatagaggg aatgttaaaa tattactccg atagcaatcg ctcttttgct
      601 cttgtatcca gtgaaggggg ggtaattttt gacagccggg ccatgagtaa actgggaatt
      661 attaacactt tgtgggatgg aggttctctt ttcatcgaca ggaaatcatc tcccggaatt
      721 aatttgaagg agccaagact gacgatatcg gtgatgattc agcctgatgt ttaccacaaa
      781 ggtttttgta cgcgaaaaaa agaaattgtg aaaacgtcag gacatcatgc aaggtttttg
      841 atgtgtcaac caacatcaac gcaggggaca aggataataa ccggagataa ttattcatca
      901 cagtatcagg atttgtttga agagaggatc aatgagctta ttgatgaaag tctggcgatg
      961 agtggtgaac gacgttgcct tcacttttct cctcaggctg ccagaatctg gacggattat
     1021 tacaatgatg tggaatcgaa gctgggggga ttggggcctt taagacattg ccgggaatat
     1081 gctgctaaaa atgcagagta tatggcaaga ctggcaggac ttatttacca ttcgagcggt
     1141 gaagaggggg agatttcccc ttacactgca gaaatggcga gagaattagc aatatggtac
     1201 ggtaatgagt atgtgcggtt gtctaatccg ttaacttttg acaactctgc cctgaccgta
     1261 cctgtgcgac tcattccaga ggagcttgaa cttttcaact ggataaaaag ctattgtatt
     1321 gagaagggga tcctctgtat gaaaaaaaat gacattttac agcgtggtcc gaaccgtttc
     1381 cggaaaaagg ataaaatcaa ctggttactg gatttactgt atgaacaaaa cagagttgta
     1441 ccggttattg agggaaaaac gttgtgtgtc gcacctaact ttgacctctg aacgacttaa
     1501 tcgttgagta acatatagtc tcctctgcta cttgcggagg agactaatgc ctttaccacg
     1561 tcagcgttgt tgtattagaa aactctggta caggtcagtc agtaaatata actggtgtaa
     1621 ccactattta ctattggata gagtgttgct caggatattg tcggctttag tctgaggtga
     1681 ggtgggacct tcattgtttt cgtgttcaat aaataattta ggttaattag tattattaat
     1741 gggagttatg ttatctgtta tgttatctta tctctctctt tctatcgtaa tatcactcct
     1801 ctttatgcta agtattctgt atgtgcgtaa tgcatatatc tccatacact acagtgtggt
     1861 ggtgtatgga gtttagttat gtgtaaatta tgttactaca cttatagcgt aatattgaga
     1921 aag
//
LOCUS       DAZFWL020000342         1889 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFWL020000342 DAZFWL020000000
VERSION     DAZFWL020000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.347"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..410
                     /locus_tag="ACHKNP_RS26475"
                     /old_locus_tag="pgaptmp_005295"
     CDS             <1..410
                     /locus_tag="ACHKNP_RS26475"
                     /old_locus_tag="pgaptmp_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretion protein EspJ"
                     /protein_id="HGU4707241.1"
                     /translation="NQFTDLKNKTIQGHKDTVAKVIDWYNPQKNAFGIMMGTPRRSAD
                     IAKEESRNALNFMIMEKDTFNEKILNSNANLQKKYGTTENSSWVSASVGSLLDKGAKV
                     YPDISCSLRLGEPFIITLPETVRLDVNIHPLKK"
     gene            complement(423..644)
                     /locus_tag="ACHKNP_RS26480"
                     /old_locus_tag="pgaptmp_005296"
     CDS             complement(423..644)
                     /locus_tag="ACHKNP_RS26480"
                     /old_locus_tag="pgaptmp_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707242.1"
                     /translation="MDIQCTLANTFPNEHPEAFILVTSAEKFIVDGNCIQLIGIGNEQ
                     GEFDFYRQIYPVTGGEHKVEALMWTHLPA"
     gene            complement(728..1108)
                     /locus_tag="ACHKNP_RS26485"
                     /old_locus_tag="pgaptmp_005297"
     CDS             complement(728..1108)
                     /locus_tag="ACHKNP_RS26485"
                     /old_locus_tag="pgaptmp_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707243.1"
                     /translation="MNEIRSNPVKFLEEHLILNTQRYAHHDETSLITVNITREDGLLR
                     LKETEYDMAGSDYILFTAMRDTDSPERFDAPAVVSLSNQCISLRRNDISQTIQQSSPL
                     WLTNQQSGCSVLIVRHGLSESGEQ"
     gene            complement(1309..1884)
                     /locus_tag="ACHKNP_RS26490"
                     /old_locus_tag="pgaptmp_005298"
     CDS             complement(1309..1884)
                     /locus_tag="ACHKNP_RS26490"
                     /old_locus_tag="pgaptmp_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGU4707244.1"
                     /translation="MPLTSDIGSRSFNLGLEVFRARIAANGRGDITVGGETVSIVYDA
                     TNGRFSSSGGNDGLLSELLILGFNNGPRALSERMLSMLSDSGEAQSQESIQDKISQCK
                     FPVSSGNFQCPPESIQCPITLERPEEGVFVKNSDSSAVCTLFDVDALSRVVNDGSVHP
                     LTRAPITPSMIVKPEECKYDPARGSFIIKDS"
BASE COUNT          605 a          416 c          330 g          538 t
ORIGIN      
        1 gtaatcagtt tactgattta aaaaacaaga caattcaggg tcataaagac actgtcgcaa
       61 aagtcataga ctggtataac ccacaaaaaa acgcatttgg tatcatgatg ggcacaccaa
      121 gacgttctgc tgatatagca aaggaagaat ccagaaacgc tctaaatttc atgataatgg
      181 aaaaggatac atttaatgaa aagatactta acagcaatgc taatttacaa aaaaaatacg
      241 gcacaacaga aaatagtagt tgggtatcgg cttctgttgg ttctcttctg gacaaaggag
      301 ccaaagtata tccagatata tcatgctctc tgagactggg agaaccattc attattacac
      361 ttccagaaac agtcaggctg gatgttaata tccacccact caaaaaatga tcctgaacgt
      421 ttttatgcag gcaaatgcgt ccacatcaag gcttcaacct tgtgctctcc ccctgtcaca
      481 ggataaattt gcctgtaaaa atcaaactct ccctgctcat taccaatacc aatcagttga
      541 atgcagttgc catctacaat aaatttttcc gctgacgtaa ccaaaataaa ggcttccgga
      601 tgttcattag gaaaggtgtt agcaagagta cactgaatat ccatctccaa caaaatctcc
      661 tgattattag cgtaaactca tcgacgaaat cgttactatc ccctgggcac atatgtacca
      721 ttgaatacta ctgttctcca gattctgata atccatggcg aacaataaga acactacagc
      781 cgctttgctg attagttagc cataacggtg aactttgctg gatagtttga gaaatatcat
      841 ttcgccggag gcttatacac tgattactca agcttacaac tgcaggtgca tcaaagcgct
      901 ccggactatc agtatcacgc atagctgtaa acagaatata atcactacca gccatatcat
      961 attccgtttc ttttagtctt aacaagccat cctctcttgt aatattaact gtaattaaag
     1021 atgtctcatc atgatgggca tagcgctgtg tattaagaat taaatgctct tccagaaatt
     1081 ttacagggtt actacgtatt tcattcatta tacaccgaag ctctgaacga tgcggcacca
     1141 gaaagtgccc ggtattttgt gaaataatac tacttacctt gattgttttc tctccagctc
     1201 atgaaaaata actcgcgtat tatattgttt taccaaccac aggccccgaa tgcctgtggt
     1261 gtttttatat aaattatcgg aagttaatat tgtttcattt gaaacatctt aactatcttt
     1321 tataataaag cttccccttg cagggtcata tttacactct tctggtttaa caatcattga
     1381 tggggttatt ggagctcgtg tcagagggtg aactgaaccg tcattaacca cgcgagaaag
     1441 tgcatcaaca tcaaataaag tgcatactgc tgaactatct gaatttttga caaacactcc
     1501 ttcttcgggt ctctctagtg taattggaca ctgaatagac tctggcgggc actggaaatt
     1561 tcctgaacta acaggaaact tacattgaga tattttgtcc tgaatactct cttgcgattg
     1621 tgcttcacct gagtccgaaa gcatacttag cattctctca ctaagggctc gaggaccatt
     1681 attaaatccc aaaatcaata actcagaaag caatccgtca ttaccgccac tggatgaaaa
     1741 gcgcccatta gtagcatcat acacaatact gacagtttca ccacctactg taatatctcc
     1801 gcgcccattg gctgcaattc gggcacgaaa aacctccagc ccaagattaa atgaacgtga
     1861 tccaatatct gaggttaatg gcataacaa
//
LOCUS       DAZFWL020000343         1820 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFWL020000343 DAZFWL020000000
VERSION     DAZFWL020000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.349"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..351
                     /locus_tag="ACHKNP_RS26495"
                     /old_locus_tag="pgaptmp_005299"
     CDS             <1..351
                     /locus_tag="ACHKNP_RS26495"
                     /old_locus_tag="pgaptmp_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HGU4707245.1"
                     /translation="EEGYSVFVAFYDFIKKYGGLYGFHSAYKDTTINEAFFIDPERAS
                     NRIFIERVNDYIERAGYNMIPVGECSNGHITLLYGENNVIYGGLDDYLWKYGDNLSSS
                     IDILINGKEAVEIP"
     gene            371..700
                     /locus_tag="ACHKNP_RS26500"
                     /old_locus_tag="pgaptmp_005300"
                     /pseudo
     CDS             371..700
                     /locus_tag="ACHKNP_RS26500"
                     /old_locus_tag="pgaptmp_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
     gene            845..>1820
                     /locus_tag="ACHKNP_RS26505"
                     /old_locus_tag="pgaptmp_005301"
     CDS             845..>1820
                     /locus_tag="ACHKNP_RS26505"
                     /old_locus_tag="pgaptmp_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HGU4707246.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAESW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSND
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEGKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIR"
BASE COUNT          571 a          343 c          425 g          481 t
ORIGIN      
        1 gaagaaggat attctgtttt cgttgcgttc tatgatttta taaaaaaata tggcggttta
       61 tatggctttc attcagctta caaagacacc actataaatg aggctttttt tatagatcca
      121 gaaagggcct ctaatcgcat ttttatagaa agagtcaatg attatattga gagagctggt
      181 tacaatatga ttcctgttgg cgagtgcagt aatggtcata tcacattatt atatggggaa
      241 aataatgtga tatatggcgg cttggatgat tatctttgga agtatgggga taatttatct
      301 tcatcaattg atatattgat taatggcaag gaggcagttg aaattccgta aggccaactt
      361 gatatgacac ctggcacaca accccgacga ccggatgtat atgctgggcg ggatgagtga
      421 caccgccagc aaccgcatca cgtttgagcg cgacagccag taccggatca cgtgtgtcag
      481 tcacaccgac gggatccggc ttaaactgac gtaccatgcc agcggctacc tgaaagccat
      541 tcaccgcacg gataacggca tacagacgct ggcgacctac gaacaggatg cgcgggggcg
      601 gctgaccgaa gcggatgcgc ggctggacta ccacctgttt tatgagtacg acgctgcgga
      661 ccggatcatc cgctggtccg ataacgacca gacgtggagc cagggaaaga tcatgtaacg
      721 ccggtttttt gtactaaata attcgcacta aatgttaaat aattgagata ttccttatta
      781 tctaaaactg tttttctcag cttatatatg atcaaatact ccttgtataa ataatgagaa
      841 caaaatggaa cttaaaaaat tgatggaaca tatttctatt attcccgatt acagacaagc
      901 ctggaaagtg gaacataaat tatcagatat tctactgttg actatttgcg ccgttatttc
      961 tggtgcagaa agttgggaag atatagagga tttcggggaa acacatctcg atttcttgaa
     1021 gcaatatggt gattttgaaa atggtattcc tgttcacgat actattgcca gagttgtatc
     1081 ctgtatcagt cctgcaaaat ttcacgagtg ctttattaac tggatgcgtg actgccattc
     1141 atcaaatgat aaagacgtca ttgcaattga tggaaaaacg ctccggcact cttatgacaa
     1201 gagtcgccgc aggggagcga ttcatgtcat tagtgcgttc tcaacaatgc acagtctggt
     1261 catcggacag atcaagacgg atgagaaatc taatgagatt acagctatcc cagaacttct
     1321 taacatgctg gatattaaag gaaaaatcat cacaactgat gcgatgggtt gccagaaaga
     1381 tattgcagag aagatacaaa aacagggagg tgattattta ttcgcggtaa aaggaaacca
     1441 ggggcggcta aataaagcct ttgagggaaa atttccgctg aaagaattaa ataatccaga
     1501 gcatgacagt tacgcaatca gtgaaaagag tcacggcaga gaagaaatcc gtcttcatat
     1561 tgtttgcgat gtccctgatg aacttattga tttcacgttt gaatggaaag gtctgaagaa
     1621 attatgcgtg gcagtctcct ttcggtcaat aatagcagaa caaaagaaag agcccgaaat
     1681 gacggtcaga tattatatca gttctgctga tttaaccgca gagaagttcg ccacagcaat
     1741 ccgaaaccac tggcacgtgg agaataagct gcactggcgt ctggacgtgg taatgaatga
     1801 agacgactgc aaaataagaa
//
LOCUS       DAZFWL020000344         1817 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFWL020000344 DAZFWL020000000
VERSION     DAZFWL020000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.350"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..103
                     /locus_tag="ACHKNP_RS26510"
                     /old_locus_tag="pgaptmp_005302"
     CDS             <1..103
                     /locus_tag="ACHKNP_RS26510"
                     /old_locus_tag="pgaptmp_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HGU4707247.1"
                     /translation="KPMPSREELLKRKSFGSVNDNKYLNAMWRSGKK"
     gene            100..657
                     /locus_tag="ACHKNP_RS26515"
                     /old_locus_tag="pgaptmp_005303"
     CDS             100..657
                     /locus_tag="ACHKNP_RS26515"
                     /old_locus_tag="pgaptmp_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707248.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVDETNDEYLVRKFGELESKLE
                     TALRECRSAGITIDNLEAKCAKMAAENSSLKQSEKEFNDFCREEFSEWEDDITETPAT
                     DAFLAEVKTEARKEGAYFVANRMLAAWEAGFIDDTAKNAADIARMILTSTEFMANAPE
                     GDFDRSFSDGVLEDIAEQLRKGVIQ"
     gene            654..1211
                     /locus_tag="ACHKNP_RS26520"
                     /old_locus_tag="pgaptmp_005304"
     CDS             654..1211
                     /locus_tag="ACHKNP_RS26520"
                     /old_locus_tag="pgaptmp_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4707249.1"
                     /translation="MSKIDYQALREAAERAIPAMERLLMLPVDDDLLTEQELKDYGVD
                     IDALNAFKFLTGPETVLALLDERERNQQYIKRRDQKNEDIALTVGKLRVELEAVQKTS
                     AARIEAIDRTHKMFQREKDRADAAEKCIAELSASHSKLRDTMAGIHNTIRMDGGYTPL
                     AAILNAAKRAYEESASAAGIRIKGE"
     gene            1213..1350
                     /locus_tag="ACHKNP_RS26525"
                     /old_locus_tag="pgaptmp_005305"
                     /pseudo
     CDS             1213..1350
                     /locus_tag="ACHKNP_RS26525"
                     /old_locus_tag="pgaptmp_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          528 a          387 c          480 g          422 t
ORIGIN      
        1 aaagccaatg ccatcgagag aggaattgct taagagaaag agtttcggtt ctgttaatga
       61 caacaaatat ctgaatgcga tgtggcggag tgggaagaaa tgaaacaaat gacactaatt
      121 gagatggatg gttttctgaa aggtaaatgc atcccaagtg atttaaaggt tgacgaaaca
      181 aacgatgaat atcttgtccg taagttcggt gaacttgaat caaaactaga aacggcgttg
      241 cgggagtgtc gttctgctgg aatcacgatt gataaccttg aggctaaatg cgcgaagatg
      301 gctgctgaaa attcctcgct taagcaatct gagaaggaat ttaatgactt ttgtcgtgag
      361 gagtttagcg aatgggaaga tgatattact gaaaccccag ccaccgatgc ttttctggct
      421 gaagtgaaga ctgaagcacg caaggagggc gcttactttg tggcgaacag aatgctggct
      481 gcctgggaag ctggttttat tgatgatact gcgaagaacg ccgctgatat tgcccggatg
      541 attcttacct ctactgagtt tatggccaat gcgccggaag gcgattttga ccgttcattc
      601 tctgatggcg ttctcgaaga tatcgccgaa cagcttcgta aaggagtcat ccaatgagca
      661 agattgacta tcaggcactg cgtgaggcgg cagagcgtgc aattccggca atggaacgcc
      721 tgttaatgtt gccagttgat gatgatttgt taactgaaca ggaacttaag gattacggtg
      781 tggatattga tgcgctcaac gccttcaaat ttctgaccgg gccagaaacc gtgctggcac
      841 tactggatga acgggaaaga aaccagcaat acatcaaacg ccgcgaccag aagaacgagg
      901 atattgctct tacggttggg aagctgcgtg ttgagcttga agccgtgcag aagacatcag
      961 cggcacgaat cgaagctata gaccgcactc acaaaatgtt ccagcgtgaa aaagatcgag
     1021 ccgatgccgc agagaagtgc atagcagaac tgtctgctag ccacagcaaa ttgcgcgaca
     1081 caatggctgg catccacaac acaatccgaa tggatggcgg ctatacgcca ctggcagcaa
     1141 tccttaacgc tgctaaacgc gcatatgaag aatcagcaag cgcagctggc attcgcatca
     1201 aaggagagtg atatgagcac tatcactaaa gaacgtatcg aattgttcat taaatccccg
     1261 cttgataacg gacttactag tggcgaacaa atggaactgg cacggattgc tctggcatcg
     1321 ctggaagcaa aaccaatagg tgcattccac attgcagaac agcaagttga cggcacaagt
     1381 gactacctca aggatggaga atggcctatt gataatggaa ttattgaggt ctacgccgct
     1441 ccgccagtac cggtagtacc tgctgcatta cctgagaacg acgatgagga cgggcatgac
     1501 attgattatc ttgatccatc tgaagtttac gcgcttgggc gaacagctgg ctggaacgcc
     1561 tgccgcgccg ccatgcttca gggtaaatca gaacaaccac aaaacgcaca acaaaatatt
     1621 ccggaaaata ttcccggtgg caactctccg gtaactccgg atggttgggt tatggtgccg
     1681 aaggagttaa ccccggaaat gatgagagcc gttcagatta gaagtgagct tggagggtat
     1741 gctacttcca atctatctgg tgcatacaat atgttttctg agttctggaa tgttgctgtc
     1801 tcagctgcgc ctaaggt
//
LOCUS       DAZFWL020000345         1809 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFWL020000345 DAZFWL020000000
VERSION     DAZFWL020000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.351"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            13..552
                     /locus_tag="ACHKNP_RS26530"
                     /old_locus_tag="pgaptmp_005306"
     CDS             13..552
                     /locus_tag="ACHKNP_RS26530"
                     /old_locus_tag="pgaptmp_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HGU4707250.1"
                     /translation="MNWHYEKNGVRYDNVTEEDIIERIRRGELNASTLVWQQGMLEWQ
                     PVSATSLAEILKQSEVPPALPGHRIPGGVVWTLAFAPLIGYILEMWTAGLNGMSFERA
                     YDAVSEGQYWFISLILNIALGYLDERKLRKSGVDTATFGWLAWLIPVYLWRRAKILGQ
                     KPAYFWVWLVMLILVLFTI"
     gene            822..1235
                     /locus_tag="ACHKNP_RS26535"
                     /old_locus_tag="pgaptmp_005307"
     CDS             822..1235
                     /locus_tag="ACHKNP_RS26535"
                     /old_locus_tag="pgaptmp_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707251.1"
                     /translation="MKNIITILMIAVISFFTANVSANNAVPLPTNLTDVTDGTEFWCQ
                     GTDTIDGGCEYLGTSKDMQYGLVHAMGGTACWDGLYGVINFYTGKAQTIKFNNNQSCE
                     GNITAGFVTLKNGKPGVKLYDNTIHKIVGLDEIKI"
     gene            complement(1375..1514)
                     /locus_tag="ACHKNP_RS26540"
                     /old_locus_tag="pgaptmp_005308"
                     /pseudo
     CDS             complement(1375..1514)
                     /locus_tag="ACHKNP_RS26540"
                     /old_locus_tag="pgaptmp_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1482..1556
                     /locus_tag="ACHKNP_RS26545"
                     /old_locus_tag="pgaptmp_005309"
                     /pseudo
     CDS             1482..1556
                     /locus_tag="ACHKNP_RS26545"
                     /old_locus_tag="pgaptmp_005309"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1646..>1809
                     /locus_tag="ACHKNP_RS26550"
                     /old_locus_tag="pgaptmp_005310"
     CDS             1646..>1809
                     /locus_tag="ACHKNP_RS26550"
                     /old_locus_tag="pgaptmp_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HGU4707252.1"
                     /translation="MRLASRFGYTNQIRRERPLTHEELMHYVPSIFGEDRHTSRSERY
                     AYIPTITVLE"
BASE COUNT          503 a          376 c          409 g          521 t
ORIGIN      
        1 caggagaatc agatgaactg gcattatgaa aaaaatggtg tacgttatga taacgtaaca
       61 gaggaagata ttatcgaacg tatccggcgt ggtgaactga atgcatcaac attggtgtgg
      121 caacagggca tgctggaatg gcagccagtt tcagccacat cactggcgga aatactgaaa
      181 caaagtgaag ttcccccggc ccttccgggt caccgcattc ccggaggcgt tgtctggacg
      241 ctggcatttg cccctttgat tgggtacata cttgagatgt ggactgccgg gctgaacggt
      301 atgtcatttg aacgggctta tgatgcagtc agcgaaggcc agtactggtt tatctctctg
      361 attctgaata ttgctctggg atatctggat gaacgaaaac tccgtaaatc tggtgttgat
      421 actgcaacat ttggctggct ggcatggttg atcccggttt atctgtggcg tcgggcaaag
      481 atcctcgggc aaaaacctgc ttatttctgg gtctggctgg ttatgctgat actggtgtta
      541 tttacgatct gatattcttt ttcgcagagt ttgctaaata atcaggttat tagtgttctt
      601 aacaggtaaa tgatgttcat catggagcgg atatatttta tttgattcag gagacagtca
      661 gaaacatagt ataaatatat caccatgttg aaggtcatat cttcaggtat gtgtttaata
      721 aacaagtcgg gggttaatcc tgacctctcg agtggattat aagtattgca gaaacagttt
      781 ttatactttt atttaatatc agaaacaatg gagtatttat catgaaaaat atcatcacta
      841 ttttaatgat tgcagttatc agtttcttta ctgctaatgt atcagcaaac aatgccgttc
      901 ctttaccgac caacctgact gacgttactg acggtacaga attctggtgt cagggtactg
      961 atactatcga cggaggttgt gaatacctgg gcacatccaa agacatgcaa tacggtctgg
     1021 tacacgccat ggggggtaca gcgtgctggg atggtttata cggcgtaatt aatttctata
     1081 ccggtaaagc ccagactatc aaattcaaca acaatcaaag ttgtgaaggc aacatcaccg
     1141 ccggtttcgt tactctaaaa aacggtaaac ctggcgttaa actttacgac aatacgattc
     1201 ataagattgt cgggcttgac gaaatcaaaa tttaatgtat gaattatagt atcttatctg
     1261 cgcgttatta atatcaaatg taaataaatc ctctgccata atggcagggg ctttattttt
     1321 acctgaaaat acatgacggg ctgtacatta cttttatttt ccgaagatcc tttctcacgc
     1381 aaacagacag ttgctgtcat aacagcttac cgcaatgtgt ttatccagga atgcatttcc
     1441 ggcggattat ccgcaacgcg gaaggtcagc gacgctggcg ctgccggaat tctgaaccct
     1501 atgcaggcaa acaacttaat gcgtggctca ccagtgaagg gcttcccgga caataaacct
     1561 cattcatttc acccatcagg ccgcgtcttc tccgggagac gcggtttttt catttatacc
     1621 gcttaatcat tcataaggag caattatgcg attagccagt cgttttggtt atacaaacca
     1681 gatacgccgt gaacgtccac tgacacatga agagctgatg cactatgttc ccagtatttt
     1741 tggagaagac cggcatacct cccgcagtga acgctatgcg tacattccca ccatcaccgt
     1801 cctggaaaa
//
LOCUS       DAZFWL020000346         1791 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFWL020000346 DAZFWL020000000
VERSION     DAZFWL020000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.352"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            52..633
                     /locus_tag="ACHKNP_RS26555"
                     /old_locus_tag="pgaptmp_005311"
     CDS             52..633
                     /locus_tag="ACHKNP_RS26555"
                     /old_locus_tag="pgaptmp_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4707253.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
     gene            879..>1791
                     /locus_tag="ACHKNP_RS26560"
                     /old_locus_tag="pgaptmp_005312"
     CDS             879..>1791
                     /locus_tag="ACHKNP_RS26560"
                     /old_locus_tag="pgaptmp_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707254.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSQGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAI"
BASE COUNT          421 a          462 c          575 g          333 t
ORIGIN      
        1 atctgccttc acggggattt gcaacgattt ggccgccgcc tcagcctgta cgtgaacacg
       61 gcagcggaag ccatccgggc gctgtcgtta caggtgccgg gattccgccg tcagatgaac
      121 gaaggctggt accagatacg tattcgcggt gaggacacgg caccggaggc ggtgtacgcc
      181 cgtcttcacg aacagctggg tgagggaacg gtcatccaca ttgtgccgcg actggccggt
      241 gcagggaaag gcggactgca gattgtgctg ggggcggcag ccatcgtggg ctctttcttc
      301 accgccggcg caacgatggc gttatggggt tcagccctgg cagccggcgg tttttctgcc
      361 accacgatgc tgttttcact gggggccagc atgatactgg gcggtgtggc ccagatgctg
      421 gccccgaagg caaagacgcc ggagtacaaa agtacggata acggtaaaca gaacacgtat
      481 ttttcgtcac tggacaacat gattgcccag gggaacccga tgccggtgcc ttacggtgaa
      541 atgctggttg gctcccggcg aatctcccag gacatcagca cccgtgatga aggtggtggc
      601 gggaaggtgg tggttatcgg gcggcagggg taaagcataa aaaaatcccg cagtgtatgg
      661 aggctgcggg aacagaaaat gaagattaac cacagggggt tttgttttta ttggcccgaa
      721 aaaactgtaa cgcccgggaa tgatatctgc cacggggcgt acagaaaatg tgaagaaatt
      781 cagaaatttt attccgtcat gacacaggca ccctccgggg tgcctgtcgt ttttggggca
      841 taaacagatt cagacatcag acaggagagg gggacagagt gggtaaaggt ggcggcaggg
      901 cgcacacgcc ggtagaggca aaggacaatc ttaagtccac gcagatgatg agcgtgattg
      961 acgccattgg tgaagggccg attgaaggtc cggtgaaggg gctgcagagt attctggtga
     1021 acaaaacccc gctgacggac acggacggta atcctgtgat acatggtgtg acagcggtct
     1081 ggcgcgccgg ggagcaggag cagacaccgc cggaaggttt tgagtcatcc ggctctgaaa
     1141 ccgcactggg cgtggaagtg acgaaggcaa agccggtgac gcgcaccatt acgtccgcga
     1201 acattgaccg cctgcgggtc accttcgggg tgcagtcact ggtgcagacc acctcacagg
     1261 gtgaccgtaa cccggcatcc gtccgcctgc tgattcagct gcagcgtaac ggtaactggg
     1321 tgacggaaaa ggatgtcacc attaacggca agaccacctc acagttcctc gcttcggtga
     1381 ttctggataa tctgcctccc cgccccttta acatccggat ggtcagggag acggcggaca
     1441 gcaccacgga ccagctgcag aataagacgc tctggtcgtc atacaccgaa atcatcgatg
     1501 tgaaacagtg ctacccgaac acggcgattg tggggctgca ggtggatgcg gagcagtttg
     1561 gcggtcagca gatgacggtg aactaccata tccgcggtcg catcatccag gtaccgtcaa
     1621 actatgaccc ggaaaaacgc acgtacagcg gcatctggga cggcagcctg aaaccggcat
     1681 acagcaacaa cccggcctgg tgcctgtggg acatgctgac tcacccgcgc tacggcatgg
     1741 gaaaacgtct gggggcggca gacgtggaca aatgggcgct gtatgccatt g
//
LOCUS       DAZFWL020000347         1790 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFWL020000347 DAZFWL020000000
VERSION     DAZFWL020000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.353"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..914
                     /locus_tag="ACHKNP_RS26565"
                     /old_locus_tag="pgaptmp_005313"
     CDS             6..914
                     /locus_tag="ACHKNP_RS26565"
                     /old_locus_tag="pgaptmp_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HGU4707255.1"
                     /translation="MNSQLNMKINDFYLPLLSVIGTGRLYITPEGHACHAYFREVSGN
                     GIRFTLTCSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTALPEG
                     DVSVDDVQFTMLNKDVYPVIENNNGENRLNVIILEITVQSLQYLINENWQFVPHSNTI
                     FFDGYIAPGWTDYPVTELTVGDSLRLYHVDDSKERECWLVINNPLATVKLCDNNLFIA
                     DVQAADLLCTISNEAVMDRIYCVIGTIHVDIHMLRNAKKDDIIDSTGYHLFGGCRLIR
                     NNTTIAYGSIVKINEDFYFTVSLVCD"
     gene            945..1460
                     /locus_tag="ACHKNP_RS26570"
                     /old_locus_tag="pgaptmp_005314"
     CDS             945..1460
                     /locus_tag="ACHKNP_RS26570"
                     /old_locus_tag="pgaptmp_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HGU4707256.1"
                     /translation="MSSSLSGIILNTSLTSHHMSWGKFSRTLHVLNEDIVDEIQLKAR
                     TQQRHHRVYSEMADYSVVSFRGKEYSVRFIKDYKTDNYVYKVQKMTQESGCLSRLFSF
                     LSGGVTKALESKLNERHITPLSPTWFPRTPLEGVLTERGLSSLLRRVQSTENLDYSNA
                     MISNSFSNSVV"
     gene            complement(1679..>1790)
                     /locus_tag="ACHKNP_RS26575"
                     /old_locus_tag="pgaptmp_005315"
     CDS             complement(1679..>1790)
                     /locus_tag="ACHKNP_RS26575"
                     /old_locus_tag="pgaptmp_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HGU4707257.1"
                     /translation="VLLNIAFPADKVNITEFINLMENGYLLKNEVISLLS"
BASE COUNT          547 a          289 c          367 g          587 t
ORIGIN      
        1 acctattgaa ttctcaattg aatatgaaaa ttaatgattt ttacttgcca ttgttgtcag
       61 tcataggaac cggtcgcctc tatattacgc ctgaagggca tgcttgccat gcatattttc
      121 gcgaagtcag cggtaatggt atccgcttca cgttaacatg ctcaggctat gaggggcgtt
      181 tttggatatc agaagaacag ttcattcagt ggtgccagga gttattcccg tatagcgaaa
      241 gccggctgat accggaagat atgataaaac tcatgatctt atgggtaatg cagactgctt
      301 taccagaagg tgatgtgtct gttgatgatg ttcaatttac gatgctaaat aaagatgttt
      361 atccggtaat agaaaataat aatggagaaa acagacttaa tgtaattata ctcgaaataa
      421 cggtacagag tttacaatat ctaattaatg aaaattggca atttgttcca cattctaata
      481 ccattttctt tgatggatat attgctcctg ggtggactga ttacccggtt acagagctga
      541 ctgttggtga tagtttaagg ctctatcatg ttgatgacag caaagaaaga gagtgttggc
      601 ttgttattaa taacccttta gcaaccgtaa aactttgtga taataattta tttattgcgg
      661 atgtccaggc tgcagatttg ttatgtacta tcagcaatga ggctgtaatg gatcgcattt
      721 attgtgttat tggaacaatt catgttgata tacatatgtt gcgcaatgcc aaaaaagatg
      781 atattatcga ttcaactgga tatcatcttt ttggtgggtg ccggttaata cgcaataata
      841 cgactatagc atacggaagc attgtaaaaa tcaacgaaga tttttatttt actgttagtt
      901 tagtatgtga ttaatcagga ataagttata tagggagatg tattatgtcg tcatcgttat
      961 caggaataat actcaatact tcgctgacaa gtcaccatat gtcatggggc aagtttagca
     1021 gaactcttca tgtattgaat gaagatatcg tagatgaaat tcagcttaaa gcccgaacac
     1081 aacaacgcca ccatcgagta tattcagaaa tggcggatta ctccgttgtc tcctttcggg
     1141 gcaaagaata ttctgttcgt tttattaaag attacaaaac agataattat gtgtacaaag
     1201 tacaaaagat gactcaggaa agtgggtgtc tctcaagatt atttagcttt ctttctggcg
     1261 gtgtgactaa ggctttggaa agtaaactaa atgagaggca tatcactcca ctttctccaa
     1321 cctggtttcc tcgaactcca ctggaggggg tcttaacgga aagaggatta tcttctttgt
     1381 tacgtcgcgt acagtctact gaaaaccttg attatagtaa tgctatgata tcaaactctt
     1441 tctctaactc cgtagtgtga ttatcatttg agctttcctc ctggtttaac tccagaataa
     1501 ttgtgattct ggagtttttc tgtttgtttt atgtcgataa tacattgttt ttaggaaaat
     1561 atgggatgaa tattcagagt gagtttggct ggcagaagtc agagattctg tgatgaggag
     1621 gggattgagc tgaatggatg aattccttcc agctcataat ttatcaggat aaaacatctt
     1681 acgacaataa actaattact tcattcttta ataaataacc attttccatt aaattgataa
     1741 actctgtaat gttaacctta tctgcaggga atgcaatatt caaaagtacg
//
LOCUS       DAZFWL020000348         1787 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFWL020000348 DAZFWL020000000
VERSION     DAZFWL020000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.354"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            203..475
                     /locus_tag="ACHKNP_RS26580"
                     /old_locus_tag="pgaptmp_005316"
     CDS             203..475
                     /locus_tag="ACHKNP_RS26580"
                     /old_locus_tag="pgaptmp_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707258.1"
                     /translation="MTVVITYLADDNARNRRRARRQAQREQAMQEQRLARKIALKLSG
                     CVRADKAASLGSLRCKKADECSGSICLPNVAIYAAGYRKSKQLTAR"
     gene            478..840
                     /locus_tag="ACHKNP_RS26585"
                     /old_locus_tag="pgaptmp_005317"
     CDS             478..840
                     /locus_tag="ACHKNP_RS26585"
                     /old_locus_tag="pgaptmp_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination N domain protein"
                     /protein_id="HGU4707259.1"
                     /translation="MGQEEKYELKKLIGEDAIEEIAALTTAIKNIRYALNTLISSCDK
                     NSREFLILGAALGIVDAATLHLITHDDILIEPYETLLLVRQKMADAAANGDLQLYIDL
                     RKVLRRMVKTEGDIPLTK"
     gene            complement(871..1365)
                     /locus_tag="ACHKNP_RS26590"
                     /old_locus_tag="pgaptmp_005318"
     CDS             complement(871..1365)
                     /locus_tag="ACHKNP_RS26590"
                     /old_locus_tag="pgaptmp_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707260.1"
                     /translation="MDVLSGITAAKQAYDLLKTIKETRDDAVIAKAIGDLHQRITDLQ
                     MLNAELSGLYQAEKEIAMKLRDENRKIKMFVVQAENYELHTTEGGSVVYRPKSHSDPS
                     IQQHNLCAHCFGEHKISILQPSTVTIKSNGFFVHSCPRCKNEYRMYRAPDPKPVYVPP
                     LTNY"
     gene            1566..>1787
                     /locus_tag="ACHKNP_RS26595"
                     /old_locus_tag="pgaptmp_005319"
     CDS             1566..>1787
                     /locus_tag="ACHKNP_RS26595"
                     /old_locus_tag="pgaptmp_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707261.1"
                     /translation="MTKSWSVPFPESETKHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWK"
BASE COUNT          556 a          363 c          417 g          451 t
ORIGIN      
        1 gttatcttta agccatcagc aggacgctgg aagccaaacg gaacagattg gcaggctctt
       61 taacattgat gggattgtcc cgccgaaatg cgggaaccaa agagtagttg gctttggggt
      121 gacgtgaagt gcagctgcaa gacggcaacc ggaagataag cacgcggcgc gtcaccgcca
      181 aagtcaatca tcggaggtaa acatgacagt agtcattaca tatctggctg acgataacgc
      241 cagaaatcgc cgcagagcac gcagacaggc tcaacgtgaa caggcaatgc aagaacagcg
      301 attggcacga aaaattgcgc taaagctctc tggttgcgtc agagcagata aagcagcatc
      361 actcggaagc cttcgctgca agaaggcaga tgaatgcagt ggaagtattt gcctgccaaa
      421 cgtagccatt tacgcggcag gctaccggaa atcaaaacaa ctgacggcga ggtaattatg
      481 ggtcaggaag aaaaatatga gcttaaaaag ctcattggag aagacgccat agaagaaatt
      541 gcagcattaa caacagctat aaagaatatt aggtatgcgc taaatacgct tatctcctca
      601 tgcgacaaaa atagcaggga atttttgata cttggcgcag ctctaggaat agttgatgcg
      661 gcaacgcttc acctaattac tcatgacgat attcttattg agccgtatga aacattactg
      721 cttgtcaggc aaaaaatggc tgatgccgca gcaaatggag accttcaact ttacatcgac
      781 ttaaggaaag tattaaggcg aatggtcaaa actgaaggag atatccccct gacaaagtaa
      841 gggggtgaag aggccatttc aggaataaaa ttaataattt gtcagcggcg gtacatatac
      901 aggcttaggg tctggcgctc tatacatccg gtattcattc ttacagcgag gacatgaatg
      961 cacaaagaac ccattagatt ttatagtaac cgtgcttggt tgaagtatcg atattttatg
     1021 ttcgccgaag caatgtgcac aaaggttatg ctgctgaata gaaggatcgg aatgactctt
     1081 aggtcgatac acgacagaac caccttcagt tgtatgaagt tcataattct cagcttgcac
     1141 aacaaacatc ttgatttttc tattttcatc tcgaagcttc attgcgatct ccttctcggc
     1201 ctgataaagg ccagagagct ccgcattaag catctgtaag tcagttattc tttggtgtag
     1261 atcaccaata gctttagcga taacagcatc gtctctggtt tcttttattg tcttcagcag
     1321 atcataagcc tgtttcgcgg cagttatacc agataacaca tccatataaa ttactctctt
     1381 actgtagggg taagaggatt ttactatttt tctcgctgta ggggtacacg agaaccaccg
     1441 agcctgatgt ggttaaaaga caggcacaat ctttactacc gcaagccacg cagtgaaatg
     1501 ggtgtgactt gtgttggtcg ccagaaaatg aaattagaca gcaaaccact tatttgaggt
     1561 gagatatgac aaaatcatgg agcgtacctt ttcctgaatc agaaactaaa catgatggaa
     1621 tgcctgtttt ctggagattc caggcgacag ttgaagaaga tggaatcaaa atattcgcac
     1681 ttcaatatat agcttttcat cagacagagc attatgcatg gttggttcct gcgcattgga
     1741 ttgttaattt taaaccagca ccaaatcagt ggttacagga atggaaa
//
LOCUS       DAZFWL020000349         1784 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFWL020000349 DAZFWL020000000
VERSION     DAZFWL020000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.355"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..507
                     /locus_tag="ACHKNP_RS26600"
                     /old_locus_tag="pgaptmp_005320"
     CDS             <1..507
                     /locus_tag="ACHKNP_RS26600"
                     /old_locus_tag="pgaptmp_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707262.1"
                     /translation="AITANTGTLNNVTINENCVIRGKLSANQIEGDLVKTVGKAFPRN
                     NSYASGTVTVTVYDDQGFDRQIIIPPVLFRGTKHQNFNSPNQQSYWYSTCKLQVLKNG
                     VEIFHEPATDVSRVFSSVIDMPAGRGHVTLTFNVSSAGANNWTPTTYISDLLVVVMKK
                     STAGISIS"
     gene            576..1199
                     /locus_tag="ACHKNP_RS26605"
                     /old_locus_tag="pgaptmp_005321"
     CDS             576..1199
                     /locus_tag="ACHKNP_RS26605"
                     /old_locus_tag="pgaptmp_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4707263.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVETVVIDLAYEGSASGDWRTDGFIVGVGYKF"
     gene            1264..>1784
                     /locus_tag="ACHKNP_RS26610"
                     /old_locus_tag="pgaptmp_005322"
     CDS             1264..>1784
                     /locus_tag="ACHKNP_RS26610"
                     /old_locus_tag="pgaptmp_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU4707264.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEA
                     LRRFELMVEEAARHAEEAKKNAGEAETSARNAGISASQAEESAANADTSAGEASESAR
                     QAAESAASAKQSE"
BASE COUNT          434 a          415 c          546 g          389 t
ORIGIN      
        1 gcaattaccg cgaataccgg cacgctcaat aatgtcacca ttaacgagaa ctgtgtcatc
       61 agagggaaac tgtctgcaaa ccagattgaa ggcgacctgg tgaagacggt ggggaaagcc
      121 tttccccgga ataacagtta tgccagcggg acggtaaccg tcacagttta cgatgaccag
      181 ggattcgacc ggcagattat cattcccccg gtgctgtttc gcgggacgaa acaccagaat
      241 ttcaacagcc cgaatcagca gtcgtactgg tattccacct gtaagctgca ggtgctgaag
      301 aacggggttg agattttcca tgaaccggca acggatgtca gccgggtgtt ctcatcggtg
      361 atagatatgc cggcagggcg gggtcatgtc accctgacgt ttaatgtgtc gtcggccggt
      421 gcgaacaact ggacgccgac aacgtacatc agtgatttac tggttgtggt catgaaaaaa
      481 tccacggcag ggatcagtat cagctgacgg tttattaacc cggacgggca cctcaggagg
      541 tgcctttttt attgactgaa aacaaagagg taatcatgcg gcatttatac gcaacgatat
      601 tattgtttac taccctgctg gcaggaattg cctttcctgc acaggctgaa agcggacacg
      661 gtgcattttc cgtgggatat gctcaggttc acccgggcgg cgtaccggca ttgtccggta
      721 ccggtgcgcg tgcaggtgat ttaaaaggga ttaatgtgaa ataccgttat gagttcacgg
      781 atcacctggg cggcattgtc gcgctgagtt atgcatcggt gaagaaaagt gacacgatga
      841 agacgggtga aaataccttc cattatgaaa gcctgcgcgg tcgttatgtc agtctgatgg
      901 ccggccctgt ctggcagctc agtgagcggg tcagtctcta tggcatggcc gggatggcgt
      961 acacccgctg gtctgacagt gttcaggatt accggcgtga tgaagtgaaa ccggggtatg
     1021 tgaaggagac caccaccgcc agtgatggcc atactgcgcg tcatctgtcg ccggcctgga
     1081 atgccgggat tcagttcagt cccgtagaga cggtggttat tgaccttgct tatgaaggtt
     1141 ccgccagtgg cgactggcgc actgacggtt tcatcgtggg tgtcggttat aagttctgat
     1201 tagccaggta acacagtgtt atgacagccc gccggttcag gcgggctttt ttgtggggtg
     1261 aatatggcag taaagatttc aggtgtactg aaagacggca caggaaaacc ggtacagaac
     1321 tgcacaatcc agctgaaagc aaaacgtaac agtaccacgg tggtggtgaa cacgctggcc
     1381 tctgaaaatc cggatgaagc cggacgttac agcatggacg ttgagtacgg tcagtacagc
     1441 gtcattctgt tggtggaggg atttcctccg tcacatgccg ggaccatcac cgtgtatgaa
     1501 gattctcaac cggggacgct gaatgatttt ctcggtgcca tgtcggagga tgacgtccgg
     1561 ccggaggcac tgcgtcgttt tgaactgatg gtggaagaag cggcgcgtca cgctgaggag
     1621 gcgaagaaga atgccggaga ggcggagacg tcagcgagga atgccggcat atcagccagt
     1681 caggcagaag agagcgctgc aaatgctgac acttcagcag gggaggcatc ggagtcagcc
     1741 cggcaggcgg cagaaagtgc agcctcagca aagcagtcag agga
//
LOCUS       DAZFWL020000350         1777 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFWL020000350 DAZFWL020000000
VERSION     DAZFWL020000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.356"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            62..640
                     /gene="espA"
                     /locus_tag="ACHKNP_RS26615"
                     /old_locus_tag="pgaptmp_005323"
     CDS             62..640
                     /gene="espA"
                     /locus_tag="ACHKNP_RS26615"
                     /old_locus_tag="pgaptmp_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HGU4707265.1"
                     /translation="MDTSTATSVASANASTSTSTVYDLGSMSKDEVVQLFNKVGVFQA
                     ALLMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIADVQSSSDKNKKAK
                     LPQEVIDYINDPRNDITVSGISDLNAELGAGDLQTVKAAISAKSNNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDIQSLQYRTISAISLGK"
     gene            653..>1777
                     /locus_tag="ACHKNP_RS26620"
                     /old_locus_tag="pgaptmp_005324"
     CDS             653..>1777
                     /locus_tag="ACHKNP_RS26620"
                     /old_locus_tag="pgaptmp_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspD"
                     /protein_id="HGU4707266.1"
                     /translation="MLNVNSDIQSMRSGASAATATSGINQPEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSVAEDVLSKLFGGISGEVRSRTEGTEPQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMVTLLSLDASAQKVASMKNSNEIYTDGQNKALDNKTLEFKK
                     QLEEQQKAEEKAQKSKILGQVFGWLGVAVTAIAAIFNPALWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQVFGGVSIAASILTAGIGGVSSLMSKVGDVANKVGSNIVKTVTK
                     LADVFVENVVSKVAATANGFTTSARSIGTTVLNNDSAQYSILAQLSAYAVQNLTRQSE
                     NLGESAKVELDKAAAELRNQASYLQNASQLISDSARVNSRI"
BASE COUNT          524 a          360 c          427 g          466 t
ORIGIN      
        1 ttgatattgg ttttttaatc gtttttggtc ttgctaagaa agattattaa gaggtatata
       61 catggataca tcaactgcaa catcagttgc tagtgcgaac gcgagtactt cgacatcgac
      121 agtctatgac ttaggcagta tgtcgaaaga cgaagtagtt cagctattta ataaagtcgg
      181 tgtttttcag gctgcgcttc tcatgtttgc ctatatgtat caggcacaaa gcgatctgtc
      241 gattgcaaag tttgctgata tgaatgaggc atctaaggag tcaaccacag cccaaaaaat
      301 ggctaatctt gtggatgcta aaattgctga tgttcagagt agttctgaca agaataagaa
      361 agccaaactt cctcaagaag tgattgacta tataaatgat cctcgcaatg acattacagt
      421 aagtggtatt agcgatctaa atgctgaatt aggcgctggt gatttgcaaa cggtgaaggc
      481 cgctatttcg gccaaatcga ataacttgac cacggtagtg aataatagcc agcttgaaat
      541 acagcaaatg tcaaatacgt taaacctatt aacgagtgca cgttctgata ttcagtcact
      601 gcaatacaga actatttcag caatatccct tggtaaataa ccggagataa ctatgcttaa
      661 tgtaaatagc gatatccagt ctatgaggtc tggggccagc gctgctacgg ctacttcagg
      721 tattaatcag cctgaagtga cttccgcgct ggatttacaa ctggttaaat ccacggcccc
      781 atcagcaagt tggacagaaa gtaccgcttt accaacgcca ccggcaggcc actcattagt
      841 gacgccctct gttgctgagg atgtcctgag taaactgttt ggtggtatta gtggtgaagt
      901 taggagtcga acagagggaa ccgaaccaca gcgcagcact cagaatgcaa gctctggcta
      961 tccttatctt tctcaggtga ataacgttga ccctcaggcc atgatgatga tggtcaccct
     1021 gttatccctg gatgcttcag cgcagaaagt tgcgagtatg aaaaactcca acgagatcta
     1081 cacggatggg caaaataagg cgctggataa taaaacgctg gagtttaaaa agcagctcga
     1141 agaacaacag aaagccgaag agaaagcaca aaaaagtaaa attcttggtc aggtctttgg
     1201 ttggttgggc gtcgcggtaa ctgctattgc agctattttt aacccggcac tctgggctgt
     1261 tgttgccgtt agcgcaacag caatggcact gcaaactgca gttgatgtaa tgggcgataa
     1321 ggccccgcag gcgttaaaga cagcagcgca agtctttggc ggagtatcta ttgctgcaag
     1381 tattctgaca gccggcattg gcggggtgtc ttcacttatg tctaaagttg gcgatgtcgc
     1441 taacaaagtt ggttcaaaca tcgtgaaaac agttactaaa ctggccgatg tatttgttga
     1501 gaacgttgtt tcaaaagttg cggcaacggc aaatggattt acgacctctg cacgttctat
     1561 tgggacaact gtgctgaata atgattctgc gcaatacagc atattggctc agttatctgc
     1621 ttacgcggta caaaacttaa ctcgacagag tgaaaacctg ggtgagagtg cgaaggttga
     1681 gctggataaa gctgcagccg agttgcgaaa ccaggcaagc tatttacaaa atgcttctca
     1741 gttaatatcc gactcagcac gagtaaatag ccgtatt
//
LOCUS       DAZFWL020000351         1740 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFWL020000351 DAZFWL020000000
VERSION     DAZFWL020000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.357"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..282
                     /locus_tag="ACHKNP_RS26625"
                     /old_locus_tag="pgaptmp_005325"
     CDS             <1..282
                     /locus_tag="ACHKNP_RS26625"
                     /old_locus_tag="pgaptmp_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4707267.1"
                     /translation="FSATTMLFSLGASMILGGVAQMLAPKPKTPEYRATDNGRQNTYF
                     SSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKMVVIGRQA"
     gene            521..>1740
                     /locus_tag="ACHKNP_RS26630"
                     /old_locus_tag="pgaptmp_005326"
     CDS             521..>1740
                     /locus_tag="ACHKNP_RS26630"
                     /old_locus_tag="pgaptmp_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707268.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTSDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDP"
BASE COUNT          424 a          444 c          537 g          335 t
ORIGIN      
        1 ttttctgcca ccacgatgct gttttcactg ggggccagca tgatactggg tggtgtggca
       61 cagatgcttg ccccgaagcc aaaaacaccg gaatacaggg caacggataa cggcagacag
      121 aacacgtact tttcctcgct ggataacatg attgcccagg ggaacccgat gccggtgcct
      181 tacggtgaaa tgctggttgg ctcccggcga atctcccagg acatcagtac ccgtgatgaa
      241 ggcggtgacg ggaagatggt ggttatcggg cggcaggcat aaaagcgaaa aaatcccgca
      301 gtgctcacgg acaggaactg cgggagcgtt acgaagattg agtgtaagga attattctta
      361 tgtcacgaca aaaaacatta acgcagagaa attattagcg ccacagtcag tttgtgaaaa
      421 tgtgaagata ttcagaattt ttattcagtc atgatacagg catcctccgg gatgcctgtt
      481 gtttttgtgc gtaacagtta tcacagtaaa gggtgagaca atgggcaaag gtggcggcaa
      541 ggcgcacacg ccggtagagg caaaggacaa tcttaagtcc acgcagatga tgagcgtgat
      601 tgatgccatt ggtgaagggc cgattgaagg cccggtgaag gggctgcaga gtatcctggt
      661 gaacaaaacc ccgctgacgg acacggacgg caatcctgtg atacacggtg tgaccgcggt
      721 ctggcgcgcc ggggagcagg agcagacacc accggaaggc tttgagtcct ccggagcgga
      781 aaccggactg ggcgtggaag tgacgaaggc aaagccggta acgcgcacca ttacatccgc
      841 gaacattgac cgcctgcggg tcaccttcgg ggtgcagtca ctgttggaga ccacctcaaa
      901 gggcgaccgt aatccctctt ctgtccgact gctgattcag ttgcagcgta acggtaactg
      961 ggtgacggaa aaggatgtca ccattaacgg caagaccacc tcgcagtttc tggcgtcggt
     1021 gattctggat aatctgcctc cccgcccctt taacatccgg atggtcaggg agacggcgga
     1081 cagcacctcg gaccagctgc agaacagaac gctgtggtcg tcatacaccg aaatcatcga
     1141 tgtgaaacag tgctacccga acacggcgat tgtggggctg caggtggatg cggagcagtt
     1201 cggcggtcag cagatgacgg tgaactacca tatccgcggt cgcatcatcc aggtgccgtc
     1261 aaactacgac ccggaaaaac gcacttacag cggtatctgg gacggcagcc tgaaaccggc
     1321 atacagcaac aacccggcct ggtgcctgtg ggacatgctg actcacccgc gctacggcat
     1381 gggaaaacgt ctgggggcgg cagacgtgga caaatgggcg ctgtatgcca ttgcgcagta
     1441 ctgcgaccag acggtcccgg atggcttcgg gggcacagag ccgcggatga cctttaatgc
     1501 atacctgtca caacagcgta aggcgtggga cgttctcagt gatttctgct cggcgatgcg
     1561 ctgtatgccg gtatggaacg gccagacgct gacgttcgtt caggaccgcc cgtcggatgt
     1621 ggtgtggccg tacaccaaca gcgatgtggt ggtggatgat aacggcgtgg ggttccgcta
     1681 cagcttcagt gccctgaagg accgccacac ggcggtggag gtgaattaca ccgacccgca
//
LOCUS       DAZFWL020000352         1729 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFWL020000352 DAZFWL020000000
VERSION     DAZFWL020000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.358"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1285
                     /gene="rhsB"
                     /locus_tag="ACHKNP_RS26635"
                     /old_locus_tag="pgaptmp_005327"
     CDS             <1..1285
                     /gene="rhsB"
                     /locus_tag="ACHKNP_RS26635"
                     /old_locus_tag="pgaptmp_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HGU4707269.1"
                     /translation="EPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPEVTWYG
                     WDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSLADTLQQSGGEDG
                     GSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIH
                     LYHCDHRGLPLALISKEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGL
                     YYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTN
                     GLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPN
                     RGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSF
                     YLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            1257..1640
                     /gene="yhhH"
                     /locus_tag="ACHKNP_RS26640"
                     /old_locus_tag="pgaptmp_005328"
     CDS             1257..1640
                     /gene="yhhH"
                     /locus_tag="ACHKNP_RS26640"
                     /old_locus_tag="pgaptmp_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HGU4707270.1"
                     /translation="MSAEFMVICKKLLFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
BASE COUNT          529 a          349 c          462 g          389 t
ORIGIN      
        1 agagccgctg gtcgaaagtc gctatcttta cgacccgctg ggccgcaggg tggcaaaacg
       61 ggtatggcga cgtgaacggg acctgacggg ctggatgtcg ctgtcacgga aaccggaagt
      121 gacctggtac ggctgggacg gcgaccggct gaccacgata cagaacgaca gaacccgcat
      181 ccagacgatt tatcagccgg ggagcttcac gccactcatc agagttgaaa ccgccaccgg
      241 tgagctggcg aaaacgcagc gccgcagcct ggcggatacc cttcagcagt ccggcggcga
      301 agacggtggc agtgtggtgt tcccgccggt gctggtgcag atgctcgacc ggctggaaag
      361 tgaaatcctg gctgaccggg tgagtgagga aagccgccgc tggctggcat cgtgcggcct
      421 gactgtggag cagatgcaaa accagatgga cccggtgtac acgccggcgc gaaaaatcca
      481 cctgtaccac tgcgaccatc gcggcctgcc gctggccctt atcagtaagg aaggggcaac
      541 agaatggtgc gcagaatacg atgagtgggg caacctgctg aatgaagaga acccgcatca
      601 gctgcagcag cttatccgcc tgccggggca gcagtatgat gaggagtccg gcctgtatta
      661 caaccgccac cgctattatg acccgctgca ggggcgatat atcactcagg atccgattgg
      721 actgaagggg ggatggaacc tgtatggata tcaattgaat ccgatatcag acatcgaccc
      781 cctgggttta tctatgtggg aggatgcaaa atcgggggca tgtactaatg gtctttgcgg
      841 cacactatcc gctatgatag gtccagataa atttgattct atagatagca ccgcatatga
      901 cgccttaaat aaaataaata gccaatctat ttgcgaagat aaagagttcg ctggtttaat
      961 atgtaaggat aatagtggca gatatttctc aacagcacct aaccgaggag aaagaaaagg
     1021 atcatatcca ttcaatagcc cttgccctaa tggtactgag aaagtatcag cttatcatac
     1081 tcatggtgca gatagtcatg gagaatattg ggacgaaata ttttcaggta aagatgagaa
     1141 aatagttaaa agtaaagata acaatatcaa gtcattttat ttaggtacgc ccagtggtaa
     1201 ttttaaagca atagataacc acgggaagga aataacaaac agaaaaggat tacctaatgt
     1261 ctgcagagtt catggtaata tgtaaaaaac tattgtttag gaactgtgtc attgtatctt
     1321 tgtttgtttt tacatacaac acatgggcgc agtgtaataa taatattaaa ataatgcgca
     1381 agtatgaaag tgaaggtaaa tataccgtta gaaatttggt taaaaataaa gctatagcat
     1441 tggaattagc tgagatatat gttaagaatc gttatggaca ggatgccgca gaagaagaaa
     1501 aaccatacga aatcactgag ttaacaacaa gttgggttgt tgaaggtacc attcactcag
     1561 accaaattgc tggtggggtt tttattatag aaataggcaa aaatgatggg agaattctga
     1621 attttggcca cggaaaataa gtatgtaagg ataatcaaac atcgtgtgcg ctgatggcag
     1681 agtaggtgga ggactccaga cagtcaaacg atagaaaaag atagccttt
//
LOCUS       DAZFWL020000353         1728 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFWL020000353 DAZFWL020000000
VERSION     DAZFWL020000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.359"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1154)
                     /locus_tag="ACHKNP_RS26645"
                     /old_locus_tag="pgaptmp_005329"
     CDS             complement(<1..1154)
                     /locus_tag="ACHKNP_RS26645"
                     /old_locus_tag="pgaptmp_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4707271.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAITAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWICGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPD
                     IPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDRLATAILNAAGRTS"
     gene            complement(1561..1674)
                     /locus_tag="ACHKNP_RS26650"
                     /old_locus_tag="pgaptmp_005330"
     CDS             complement(1561..1674)
                     /locus_tag="ACHKNP_RS26650"
                     /old_locus_tag="pgaptmp_005330"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU4707272.1"
                     /translation="MVDFTSRIMSVLADGVLVCGIVVLLWPVIKRNSLHNA"
BASE COUNT          406 a          508 c          415 g          399 t
ORIGIN      
        1 gaggtgcgcc cggctgcgtt cagaatagcg gttgccagac gatccggaat aatgctcctg
       61 cgcgcccatg aactgaaatg tgtcgggcgg tttgatgata cctggtttcc attcgttctc
      121 gatgccgcac cgtaatatcc tgatgccgga atatccggat cttctgccgg cgcgttagtg
      181 gcggtattga caccgttacc gtctgtcatg aagggcacaa aataaacgcc ctcactctcc
      241 ctgtttttat acccgccgta cacggtgtcg tactgggtag cgtatgtatt tttccagtaa
      301 tacgtcgtgt caccacaaat ccacggcaca tctgcagcac tgccaccatg gcactgcgcg
      361 ttaaacacgg agaggtcagc acgaaactgt gtcagcatgg ctgtaaacag cgcaggttgc
      421 tgtgcgtggg tggcggcgct catgtcaaac tctccctgca tccagcacac cgccagcaac
      481 acatttttcg ggttcttctg taatgcagct ttagtgcgcg caatcaggtc ctgatataac
      541 ggtttaccca caccccagcg tgccgaatcc tggctggccc ccgtgtccgc actgaatgtc
      601 ccctccgcgc cctgggtgaa tgccgaacca ccacgacagc atggtaccag caggatcccc
      661 gcgttattcg ggatatacgg gagcagtttt ttggcaatat gtaacccctg gccgacacag
      721 ccgtactgcc ctttgctcag gtctgccttc ggatgattca gcgtactcat atcctgcaca
      781 tcatgcaggc agtggtcggc cggaataata tcgttatatc tgcaggcagc cccacccggc
      841 gtcactgtac tgcggcgcgc cagctgttta atgcgcggat ccggagcatc gtatgaatcc
      901 ggcagcggaa gcccttcacc gtaagccatg gcattggact gcccggccag tacgatgacg
      961 tagtaccact ccggctcagt tgcaccactg accaccacat caccttctgc tgtaatcgcc
     1021 tgcatcaggg tataaggggt tatggccacc ggactcccaa acggctgcca gccctctttc
     1081 agtttgtgtg tcagcttttc cgcaaggtct gacggcgacg ccgccctgac aacatcataa
     1141 tgtttaaatg tcattattcc tcccggccgg gatagtgtat taaatcagat atggagtggg
     1201 ctgtagtccg gaagcctgaa tgacacacgg ggactacagc ccaagaaatg aaaaaaggcc
     1261 acgcagttgc gcagcctgat aaaccctggt taaaatccac acgataacaa cacaacaata
     1321 tcagtatctc atgctattgc ccgaacccat tcgggcattt tttacccata aaaaagcccc
     1381 tccggagagg ggcatgtttg catgcacatt ctttttcttg catggtgccg ggtgcctccc
     1441 ggtgaattca gtatcagcac ctgaatccgc gattatcaca tatacctggt tgctgattgc
     1501 ccctccgcac agggggattc accatgcagt agtattttta ataaacagta aacaaaaaaa
     1561 tcaagcatta tgcaggctgt ttctttttat caccggccac agcaatacca caatgccgca
     1621 gaccagcacc ccatccgcca gcaccgacat gattctgctg gtgaaatcca ccatcaccac
     1681 cagaaacagc aggagtgcag ccacagccag gcgcagtttt accgtcac
//
LOCUS       DAZFWL020000354         1727 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFWL020000354 DAZFWL020000000
VERSION     DAZFWL020000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.360"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..65
                     /locus_tag="ACHKNP_RS26655"
                     /old_locus_tag="pgaptmp_005331"
     CDS             <1..65
                     /locus_tag="ACHKNP_RS26655"
                     /old_locus_tag="pgaptmp_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HGU4707273.1"
                     /translation="CCAELMSDPNSSMYEEEDDE"
     gene            58..780
                     /locus_tag="ACHKNP_RS26660"
                     /old_locus_tag="pgaptmp_005332"
     CDS             58..780
                     /locus_tag="ACHKNP_RS26660"
                     /old_locus_tag="pgaptmp_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU4707274.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEETAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            780..1070
                     /locus_tag="ACHKNP_RS26665"
                     /old_locus_tag="pgaptmp_005333"
     CDS             780..1070
                     /locus_tag="ACHKNP_RS26665"
                     /old_locus_tag="pgaptmp_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HGU4707275.1"
                     /translation="MANLRKESRGRECQVRIYGICNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            1067..1429
                     /locus_tag="ACHKNP_RS26670"
                     /old_locus_tag="pgaptmp_005334"
     CDS             1067..1429
                     /locus_tag="ACHKNP_RS26670"
                     /old_locus_tag="pgaptmp_005334"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4707276.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIHQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENA"
     gene            1426..1614
                     /locus_tag="ACHKNP_RS26675"
                     /old_locus_tag="pgaptmp_005335"
     CDS             1426..1614
                     /locus_tag="ACHKNP_RS26675"
                     /old_locus_tag="pgaptmp_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HGU4707277.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            1611..1682
                     /locus_tag="ACHKNP_RS26680"
                     /old_locus_tag="pgaptmp_005336"
                     /pseudo
     CDS             1611..1682
                     /locus_tag="ACHKNP_RS26680"
                     /old_locus_tag="pgaptmp_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
BASE COUNT          518 a          395 c          438 g          376 t
ORIGIN      
        1 actgctgcgc agaactgatg agcgatccga atagctcaat gtacgaggaa gaagacgatg
       61 aatgagttaa taaatggcaa tgccatcaaa atgacaagca ttgaaatagc tgagttggtg
      121 ggtaagcgtc atgacaatgt gaaacgtacc atcgaaacgc tggctaaaaa tggtgttatc
      181 cggcttcctc aaattgaggt ttccgaaaga atcaataact tagggttcaa tgttcagtac
      241 gagcattacg tcttcgaggg cgaacaaggt aagcgagaca gtattgtcgt tgttgcccag
      301 ttgtcgccag agttcaccgc tcgtcttgtt gaccgttggc gagagcttga agaaactgcg
      361 gttaatatcc ccaaaacgtt accggaagcg ttgcgccttg ctgctgatct tgctgagcag
      421 aaaatgcaac tggaaaacca gctcgcaatt gccgcaccta aagttgagtt tgccgatcgc
      481 gttggcgagg ccagcggaat tttgattgga aactttgcaa aggttgttgg tattggtcca
      541 aacaaactgt ttgcgtggat gcgcgatcac aaaatcctta ttgcttcagg ttcccggcgc
      601 aatgtgccaa tgcaggaata tatggatcgc ggctatttca cagtgaaaga aacagcggtc
      661 aacacaaatc acggaataca gatatcgttc accacaaaaa tcaccgggcg tggtcaacag
      721 tggctgacca gaaagctgct cgataacgga atgctgaaag taacagggga ggctgcttaa
      781 tggctaacct acgcaaagaa tctcgcggca gagaatgcca ggtacgtatt tacggcatat
      841 gcaatggcaa tcctgaaact acagttctgg cacattaccg gatggctgga atttgcggaa
      901 cgggaatgaa gcctgacgac ctgatcggcg catgggcttg tagcgcgtgt cacgatgaaa
      961 tcgaccgacg cacccataac ctcgacaaca aagacgccag actttaccac ctcgaaggcg
     1021 tgatcaggac gcaggcgata ctgctgaagg aggggaagat taagtcatga acgaatatca
     1081 gtttgtgctt ccatacccgc cgtcggtgaa tacctactgg cgaagacggg gaagccaata
     1141 ctacatcagc gataaaggcc agaaataccg aaaagacgtt cagcaaatca tccaccaact
     1201 caagttagat attttcacca aatcacgact ccgcatcaaa gtaatcgcag acgttccaga
     1261 ctcccgccgc cgcgacctag ataacatcct gaagggttta ctcgactccc ttatccatgc
     1321 cggatttgcg gaagacgacg agcaattcga tgacattcgc gtaattcgtg gtgtgaaagt
     1381 accaggcgga cggcttggaa taaaaatcac cgaactggag aacgcatgaa cgccacaatt
     1441 caaacgatac cagagcttct tatccagaca cgaggcaatc agaccgaagt ggcgaggatg
     1501 ctttcctgcg caagaggaac agtgctcaag tacaaccgag acagcaaagg cgagcgtcat
     1561 gtaatagtta acggcgtcct gatggtcaaa cagggcaaga ggggaagacg atgagactcg
     1621 aaagcgtagc taaatttcat tcgccaaaaa gcccgatgat gagcgactca ctactggcca
     1681 cagtttattg gttttcgtaa ctgagtcatt ttattatttt attgcaa
//
LOCUS       DAZFWL020000355         1720 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFWL020000355 DAZFWL020000000
VERSION     DAZFWL020000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.361"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(131..241)
                     /locus_tag="ACHKNP_RS26685"
                     /old_locus_tag="pgaptmp_005337"
     CDS             complement(131..241)
                     /locus_tag="ACHKNP_RS26685"
                     /old_locus_tag="pgaptmp_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707278.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHHANTIKLIFEM"
     gene            complement(294..698)
                     /locus_tag="ACHKNP_RS26690"
                     /old_locus_tag="pgaptmp_005338"
     CDS             complement(294..698)
                     /locus_tag="ACHKNP_RS26690"
                     /old_locus_tag="pgaptmp_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HGU4707279.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(919..1650)
                     /gene="bluR"
                     /locus_tag="ACHKNP_RS26695"
                     /old_locus_tag="pgaptmp_005339"
     CDS             complement(919..1650)
                     /gene="bluR"
                     /locus_tag="ACHKNP_RS26695"
                     /old_locus_tag="pgaptmp_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HGU4707280.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          474 a          369 c          361 g          516 t
ORIGIN      
        1 aaatacatat aactggatga tgactaaacc aaaacacatg tgcgttaagt attgaaacgg
       61 gcgtatggca cacccgttgt ttttataaat atattaaccg ttataaaata acgtatagaa
      121 agtcaagtga tcacatttca aatatcaatt tgatagtatt ggcatggtga ttatttatgg
      181 gtagcaataa aaggacagta tttatcatcc atagggatag tctctgtact tttattccca
      241 ttatgctaat gccttactga attatgaagc atttcttaag catccaactt tagctagatt
      301 aatggtttat tattttctac atcttcaata tataaaagcg tattatcaat ggcgtagtaa
      361 ctgcgtttgt tatgattaac atcagtaacc caccggaaaa cgcccgcgcc tgctagtgtt
      421 gaacagtatt cccgaaatgt agattttccg caaatatgaa gcaatgcggc ctcttttatt
      481 ttagcagggt tcttggtcgt actaactttt aacaggttcc tggttcctct taataacaaa
      541 accgtgtcat cgtgagtaat aattctgatg ttatccgtag ccagataata aatgtaatgt
      601 gcaatacggt gatgttttaa ttctgaataa aaccaggaga agttttgctc ttttctcact
      661 tgctcaaaca tcttttgaaa aacaacgacc tgatccatca ggataataac ctcttgttag
      721 ttgtgagact gcgtagtgtg cacgatcggt tttaccactt caatctggtc tgtcctttgg
      781 ctgtgatatg tacagagtgt gatagaggga atatctgaat tctcccggtg agcattttgc
      841 aacggaccag ctccggtaca aacgctgttg tgggttcaga ttataacatt ctgtctaagg
      901 ggcgggataa aggtgaaatt agggggcatg aaagatgact ttataacctt gctcacccca
      961 atgttgtaaa agttcgtttt gccttctcgt tggtgccatg cctgtccaga caatcaatgt
     1021 ttgcgtcggg aacagttccg ggcgcggcga ttcaatggga tcagcaagaa cagaaatgtg
     1081 ccatcctgac aatgataaac gccatgcttc cagccacaat cgtgctctct cttcaacatt
     1141 ccacgccatc agaatggctt ctttaccggg cttacggcgc atttcgaaaa gcgaggttgc
     1201 tgcgtattca attaatgcgc cgtcaaacat actgctcata atgcgggagg tgttgtgatc
     1261 aagcacgaga cgctggcgaa caggaaggta aacatgatta atcaattgat ctactgggta
     1321 ctctcgaccc agtgaaataa ttctcgcgcg tagtttggca ggattagcca tgcgaagaat
     1381 tgacatcatc tcttcttgca ggcggctcca gtcatcttcc gtatcctggc tggtggtttc
     1441 cagtaatgct ttaactttgc ctacagggac gccattactt atccaacgct tgatctcttc
     1501 gatgcgttgt atgtcttctt catcaaagag tcggtgtccg ccttcactgc gctgtggttt
     1561 taacaaaccg tagcggcgtt gccaggcccg gagagtgaca ggattaatcc cgcaacgttc
     1621 agcaacatca ccaatgctgt aataagccac aattcctcct tgcggtcaca aatctccgtc
     1681 gcctgtacac gacccaataa tactttgtac aatatacgct
//
LOCUS       DAZFWL020000356         1708 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFWL020000356 DAZFWL020000000
VERSION     DAZFWL020000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.362"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1708
                     /locus_tag="ACHKNP_RS26700"
                     /old_locus_tag="pgaptmp_005340"
     CDS             <1..>1708
                     /locus_tag="ACHKNP_RS26700"
                     /old_locus_tag="pgaptmp_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HGU4707281.1"
                     /translation="SKINSDTVEVTVTDLQGRLYSVDIDTRVIGLTFKEGINSLSEAL
                     EHMNIDAIMSVIGLVQYTRMIKTNDNISAIDHAGAVSDIKNIVDKFLGGILTFTNNRV
                     YNPGGISGASLEGFISSGLEVCASRIGGTAGRYLSNVAKVIKLPLLDIGINIWSLYDS
                     SLNHARATTQIEYISTAIDVSFSSINTALSIGAIAYPPLAIAIVPITIFSHEVKNYAV
                     YVNQINERHKLWLEAEKYLDNGSAKVLSINKDTGIIDLSNNQVLGNVHLDMRENPPIL
                     HGEKSYNSGKNIGSHPDMTDREIMESGAYNFACTKLSDAGEPDIFGWGDKDVCNSMEL
                     SESQLANGYSNRQWPSQSPVIPEGIYNTVYLGYGETLRANTEVTLSSTGYFYEIARAY
                     TDDELSEPLLTVCNQRSSVIGGKEPLTIIIPALEHGMLGSNLHMIERFKNYNFSISGG
                     KGGIKLMVGGIGDYNIECTPGVRNIISFEQLSRDFNLDLDLSDERKQNFYFHHPSGFY
                     SGKVMSITQKGINSVVGTKQGYDKIRGNNLDNTFSLGNGGGVIYSGGGSNTYFVPATL
                     QHN"
BASE COUNT          576 a          252 c          350 g          530 t
ORIGIN      
        1 tcaaagataa atagtgatac tgtggaggtt acagtaactg atctgcaagg aagactttat
       61 agtgttgata tagatacaag agttattggc ttaactttta aagaggggat aaatagttta
      121 tctgaagcac tcgaacacat gaatatagat gcaattatgt ctgttattgg cttggttcaa
      181 tatacacgaa tgataaaaac aaatgacaat attagcgcaa tagatcatgc aggagcagta
      241 tcagatatta aaaatattgt agacaaattt ttaggcggaa ttttaacttt tactaataat
      301 cgcgtatata accctggtgg tatatctggt gcctcgcttg aaggatttat atcttctggt
      361 ttagaagtgt gcgcgtcaag aataggtggc accgctgggc gttatcttag caatgtggca
      421 aaagtaataa aattaccttt gctagatata ggcataaata tttggtcatt atatgactct
      481 tcattaaatc atgccagagc caccacacaa attgaatata tatcaacagc gatagatgta
      541 tctttttcat ctataaatac tgctttatca ataggcgcaa tcgcatatcc acctttggca
      601 atagctatag ttccgataac tatcttttct catgaagtaa aaaactatgc tgtttatgtg
      661 aatcaaatta atgagcgaca taagttatgg cttgaagctg aaaaatatct ggacaatgga
      721 agcgcaaagg ttctaagcat taacaaagat acaggcataa tcgatttatc aaataatcag
      781 gtgttaggta atgttcattt agatatgaga gaaaaccctc cgatattaca tggtgaaaaa
      841 tcatataata gcgggaagaa tattggaagt catccagata tgactgacag agaaattatg
      901 gagtctggag catacaattt tgcatgtaca aaactctcag atgcaggtga acccgatatt
      961 ttcggttggg gagataagga tgtatgtaat tcaatggaac tgtctgaatc tcaattagct
     1021 aatgggtatt ctaaccgcca atggccttca caaagtccag ttattcctga agggatttat
     1081 aatacagtat atcttgggta tggagaaaca ttgcgagcta atacagaagt tacattgagt
     1141 agcactggtt acttttacga gattgcaagg gcatatactg atgatgaact atctgagcca
     1201 ttattaacag tatgtaacca acgtagtagt gtaattggtg gaaaagaacc attaacaata
     1261 atcattccag ctcttgaaca tggtatgctt ggctctaatc tacatatgat tgaacgtttt
     1321 aaaaactaca atttttcaat atcaggaggg aagggcggga taaaattaat ggttggtggt
     1381 attggtgatt acaacataga atgtaccccc ggtgttagaa atattatttc ttttgaacaa
     1441 ttatcacgtg acttcaatct ggatttggat ttatcagatg agaggaaaca aaatttttac
     1501 tttcatcatc cctctggttt ttattcagga aaagttatga gtataacgca aaagggcata
     1561 aattcggttg ttggtactaa acagggatat gataagattc gtggtaataa tttagataat
     1621 accttttccc ttggaaatgg ggggggggtt atatattctg gtggaggaag caatacatat
     1681 tttgttcctg ctacactaca gcataatt
//
LOCUS       DAZFWL020000357         1701 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFWL020000357 DAZFWL020000000
VERSION     DAZFWL020000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.363"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..67)
                     /locus_tag="ACHKNP_RS26705"
                     /old_locus_tag="pgaptmp_005341"
     CDS             complement(<1..67)
                     /locus_tag="ACHKNP_RS26705"
                     /old_locus_tag="pgaptmp_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707282.1"
                     /translation="MKHTDIRAAVLDALEQHEHGAT"
     gene            complement(64..639)
                     /locus_tag="ACHKNP_RS26710"
                     /old_locus_tag="pgaptmp_005342"
     CDS             complement(64..639)
                     /locus_tag="ACHKNP_RS26710"
                     /old_locus_tag="pgaptmp_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707283.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPSASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPAIKLGIRMLGHRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPEARYAKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVNRIRRERLPKELGYALKQQLRIVIKR"
     gene            complement(654..1007)
                     /locus_tag="ACHKNP_RS26715"
                     /old_locus_tag="pgaptmp_005343"
     CDS             complement(654..1007)
                     /locus_tag="ACHKNP_RS26715"
                     /old_locus_tag="pgaptmp_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4707284.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGISAQFTSGAQRGGEVQGVFD
                     DPESLGFASSGIRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHKNP_RS26720"
                     /old_locus_tag="pgaptmp_005344"
     CDS             complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHKNP_RS26720"
                     /old_locus_tag="pgaptmp_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4707285.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEMQS
                     SGDDVQVMNMDIRERENAAHDVREETSGALTRIRVLTCLHLCGVDGETGESVELADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(1426..>1701)
                     /locus_tag="ACHKNP_RS26725"
                     /old_locus_tag="pgaptmp_005345"
     CDS             complement(1426..>1701)
                     /locus_tag="ACHKNP_RS26725"
                     /old_locus_tag="pgaptmp_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4707286.1"
                     /translation="VWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGD
                     LAIVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
BASE COUNT          389 a          530 c          375 g          407 t
ORIGIN      
        1 gcgtcgcccc gtgttcatgc tgctcgagtg catccagcac tgcagcacgg atatcagtat
       61 gtttcatcgc tttatcacaa tccttagttg ttgtttcaac gcatagccca gttcttttgg
      121 cagacgttct cgccggatac ggttaacgtt ctcatcaaaa gcctgcttca gtggggccgc
      181 catcggaatt ttaaccacct gaatgggaag acgattacgc tttttccttc ctttatcgtc
      241 attgcccttc gcataccggg cttctggcaa acgttgcata acatgccagc gcccattatt
      301 taatcgctgg atgaatgccc gctgataacg atgctgaccg gctttaagta tgctgttcgg
      361 acgatgacca agcatcctga tccccagctt aatagcaggg agatcaccgc ggttaacgat
      421 aattctggca ttcggatttc tgaccgtcgc ccgtttcagt ctggaccgtt cctttaccag
      481 tttccggctc accctggtct cccgggcaac ctgtgatgaa gactgattaa tcgccgttgt
      541 agccacgcgg ttaatagcca ttgctgaagc cgacggaatg gcgtttttac gaacccggct
      601 cagattttca atcgcctgat caagcccttt tatcgccata attcaccctg cgtttatcgt
      661 cgccggttaa ctgcgggtgg ttgcccacgg ttcagccaga gataacaact gcccccgtca
      721 tccggagaaa cacgatccac ccagaacgtc tcaccattaa tggtcaacgt gtcaccacgc
      781 cgcacggcac gaaccgtatc cgtccgcaca aataatgacg ggctgcttcc ttcaatacgg
      841 atcccactac tggcaaaccc cagcgactcc ggatcgtcaa aaaccccctg aacttcgccg
      901 ccacgctgtg caccggaggt gaactgcgca ctgatgccca tcacttcaac aatcgtactg
      961 tccaccccgg caagggcggc atcaaaggca ttctgaaaat cacgcataaa cagccattcc
     1021 accatcaacg tgtgtttttg catctgagga cataatcaga atcacccgac caacatccgc
     1081 aagctcaacg gattcccccg tttcaccatc aacgccacag agatggaggc aggtcagaac
     1141 tctgatgcgc gttaacgcgc cggatgtttc ctcacgaaca tcatgagccg cgttttccct
     1201 ctcccggata tccatattca taacctgtac atcatcgccg gatgactgca tttcctcttc
     1261 ccattctgcc acccgctgcg ctatctctgc ggcactcccg gatatatccg gctcacgccc
     1321 cagaatcagg gccagttcat caagccgttt cagattttgc tctttcgttg ccatatcatc
     1381 cccctgtgaa aaaagacacg ggggcatttc gcccccgctc acggattatt tcacctgtac
     1441 caccacaaac tcatccgggt ccggcaacac catcagcggc gcggactgcg tcatggtaaa
     1501 ttcacgggcg ggatccccta ccgtcagcca gtgtttcgga taacgggaag aggccaccac
     1561 aatggccaga tcgccgtaat accctttgaa ggacaccacc gcgcccaggt ctttcactgc
     1621 cgtttcgagt tgtgaatttg agccgcgacg ggtatccagt ttttcgcgga acaatttaaa
     1681 gccattcagc agacgccaga c
//
LOCUS       DAZFWL020000358         1682 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFWL020000358 DAZFWL020000000
VERSION     DAZFWL020000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.364"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..882
                     /locus_tag="ACHKNP_RS26730"
                     /old_locus_tag="pgaptmp_005346"
     CDS             <1..882
                     /locus_tag="ACHKNP_RS26730"
                     /old_locus_tag="pgaptmp_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4707287.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALNLSRLPELLSRIDGYKGQPVTRLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     KGISPFEYANVNNPLRR"
     gene            1173..1526
                     /locus_tag="ACHKNP_RS26735"
                     /old_locus_tag="pgaptmp_005347"
                     /pseudo
     CDS             1173..1526
                     /locus_tag="ACHKNP_RS26735"
                     /old_locus_tag="pgaptmp_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          459 a          399 c          402 g          422 t
ORIGIN      
        1 ttccccttta tcggcaaccg ggatatcaca acactcaata ccccggacct gcttatccct
       61 gttcgtgctg cagaagcaaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata
      121 tctgctgtaa tgcgttacgc cgtacagtct ggcatcatca gatataaccc ggctctggat
      181 atggctggtg cattgaccac ggtaaaacgc cagcatcgcc ccgctctgaa tctttcacgc
      241 ctgcctgaac ttctgtcgcg tattgacggt tataaaggcc agcctgtcac ccggcttgcc
      301 gttatgctga atttactggt ttttattcgt tccagtgaac tcagatatgc ccgctggtct
      361 gaaattgata ttgacaatgc catgtggact attccagccg aacgcgaacc tctgccaggc
      421 gtaaaattct cacaccgggg ctccaagatg cgaacaccac atcttgtgcc actcagcaaa
      481 caggctgtag ccatactgac agaactacag acatgggctg gtgaaaatgg tctgatcttt
      541 acgggagcac atgacccgcg taaaccaatc agtgaaaata ctgtaaataa agccctgagg
      601 gtgatggggt atgacacaac ccaggatgtc tgtggccatg gattccgggc gatggcgtgc
      661 agtgcattga ttgaatcagg tttgtggtcc cgcgatgctg tggaacgtca gatgagccat
      721 caggagcgta atggtgtacg tgctgcgtat atccataaag cagaacatct ggaagaacgt
      781 cgactgatgc tacagtggtg ggccgatttt cttgatgcga accgggagaa ggggatcagt
      841 ccgtttgaat acgcaaatgt taacaatccg ctgagaaggt gacagaaggc tattcacaca
      901 catgctcttc ccatggggaa gagcaacctg ctccaataaa tttctttttt attccggctg
      961 atagcaccgc aatcacccgt tacctctaaa tagccactta gcattcggcg tataagttga
     1021 cagagaagga gtatctcagc aggagcttaa agtgtggttt taggcagaat ttgattgatc
     1081 ctccatttgg ggcatgtgac taatcgatat agcttcctga acggaaaaag tgttttccct
     1141 tccaatataa actattcgac aataatataa atcaattaca gaataaaact ctgtataaac
     1201 atgcaaaaat ggtaaaggcg ttcgactata tcctgaatca ctggaatgcg ctgaacgagt
     1261 tctgtcgtga cggctgggtg gaaatagaca acaacatcgg tgaaaacgcg ttacgatcgg
     1321 tggcggttgg aagaaaaaat tatctcttct tcggctcaga caagggggga gaaagcgcgg
     1381 cgatcatcta cagtctgctg gtcacctgca aactgaacga agtggagccg gaggactggt
     1441 tgcgcgaagt gatcgtgaag ctcaatgact ggccgtcgaa ccgtgtgcat gaactgctgc
     1501 cctggaactg ctcaactgta aaataatcct tacgctacgt acttctcggg gcgcttacga
     1561 atttttcacg tgccacagaa ttttgaacgc tttctcttac aatatttcaa tgtttctatc
     1621 agtattcgcc ggaagaagtc atcgaccaaa tcatcccagt cgtctcgcac cactgaccat
     1681 tc
//
LOCUS       DAZFWL020000359         1677 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFWL020000359 DAZFWL020000000
VERSION     DAZFWL020000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.365"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..498)
                     /locus_tag="ACHKNP_RS26740"
                     /old_locus_tag="pgaptmp_005348"
     CDS             complement(<1..498)
                     /locus_tag="ACHKNP_RS26740"
                     /old_locus_tag="pgaptmp_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707288.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFE"
     gene            complement(751..1374)
                     /locus_tag="ACHKNP_RS26745"
                     /old_locus_tag="pgaptmp_005349"
     CDS             complement(751..1374)
                     /locus_tag="ACHKNP_RS26745"
                     /old_locus_tag="pgaptmp_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4707289.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            complement(1371..>1677)
                     /locus_tag="ACHKNP_RS26750"
                     /old_locus_tag="pgaptmp_005350"
     CDS             complement(1371..>1677)
                     /locus_tag="ACHKNP_RS26750"
                     /old_locus_tag="pgaptmp_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HGU4707290.1"
                     /translation="ELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIWNRER
                     ISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGA"
BASE COUNT          485 a          416 c          328 g          448 t
ORIGIN      
        1 ctcaaaaaaa tgttttttta taaatggctt atcatagaag catgataccg ataaaaatcg
       61 cccataagat cttatcgaaa ttgtaaccgc cagcgcaatc gctgctgaca gtagcaaaag
      121 aggtacctga atcttctgtc tcaatataga aaactcaata attgccggca caaacaataa
      181 ttccacagca aaataaaggc gaaatacatt tagctcttgc atagaatgtt ttcttttcac
      241 tgcgaaaaag aatacaacac caatacccca accgataaga aatatagcaa tgacgataac
      301 tgcaaaaaat aaacttctgg caacatcatc aacacctgca cctacaatcc accatgggaa
      361 gccgtagtaa aaagaagtac cccatccata gaaataagca ctcccccatc caaggcatcc
      421 catgtaggca ataaaaagtg aagaactcct gagcagcgca ccatccttca taaccacccc
      481 aatacaagat gataacattg gcttacaact cataacaaaa gcaattcaat gccgtcaaga
      541 ggttacaggc taaaaaaact ctattacatt gcagtcagca tgtttactac acaaatacaa
      601 ttcagagcat aaaaactact cggcggcagg ttattgagac tcatcaatga catgtaaaaa
      661 acgcccatta ttggtgtcaa gtttccccaa agttattcaa aaagtcaata ttatgccgtt
      721 aatatgttgc catccgtggc aatcatggcg ctaacgtgtg atcgcattca aaatgttgtc
      781 tgcgattgac tcttccttgt ggcattgcac aaccagagcg tcatacagcg gcttaacagt
      841 gcgtgaccag gtgggttggg taaggtttgg gattagcatc gtcacagcgc gatatgcggc
      901 gcttgctggc attcttgaat aaccgacgcc tttacatctt ccgcactctt tctcagcaac
      961 tatcccccac tgctctgttt tggctatatc aaccgcacgg cctgtaccgt ggcaatctct
     1021 gcatcttgcg cccggcgtag cggcactacg gcaataatcc gcataagcga atgttgcgag
     1081 cacttgcagt acctttgcct tagtatttcc ttcgagcttt gccacaccac ggtatttccc
     1141 cgataccttg tgtgcaaatt gcatcagata gttgatagcc ttttgtttgt cgttctggct
     1201 gagttcatgc ttaccgcaga atgcagccat tccgaatccg gcttgtgatt gcgccatccc
     1261 catagcagcc atcacatcag taccagaaag agagtcagaa gccgtagccc gtggtgaatc
     1321 gctcatcatc gggctttttg gcgaatgaaa tttagctacg ctttcgagtc tcatgcgcct
     1381 tctccctgta cctgaatcaa tgtgaggttt ccgcagaaca ctgcgccagt atcgatatac
     1441 atctggttgg caaatttgag tggtttcact gctggcgtat gaccaaagat gaacgtgtcc
     1501 gcgcctttga tttctttcac gatcccgtct tgtgagttgc tgattcgttc gcggttccag
     1561 attacctgct gatgatcaac tggctttcca aactcgtatt tatcacaagg ataatcggcg
     1621 tggcagatga catatttttt tcctttactc accagttcga tgattaacgg aagttca
//
LOCUS       DAZFWL020000360         1673 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFWL020000360 DAZFWL020000000
VERSION     DAZFWL020000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.366"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..1670
                     /gene="tir"
                     /locus_tag="ACHKNP_RS26755"
                     /old_locus_tag="pgaptmp_005351"
     CDS             54..1670
                     /gene="tir"
                     /locus_tag="ACHKNP_RS26755"
                     /old_locus_tag="pgaptmp_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HGU4707291.1"
                     /translation="MPIGNLGHNPNVRALIPPAPPLPSQTDGAGGARNQLINSNGPMG
                     SRLLFTPIRNSVADAADSRASDIPGLPTNPLRFAASEVSLHGALEVLHDKGGLDTLNS
                     AIGSSLFRVETRDDGSHVAIGQKNGLETTVVLSEQEFSSLQSLDPEGKNKFVFTGGRG
                     GAGHAMVTVASDIAEARQRIIDKLEPKDTKETKEPGDPNSGEGKIIEIHTSTSTSSLR
                     ADPKLWLSLGTIAAGLIGMAATGIAQAVALTPEPDDPITTDPDAAANTAEAAAKDQLT
                     KEAFQNPDNQKVNIDENGNAIPSGELKDDVVAQIAEQAKAAGEQARQEAIESNSQAQQ
                     KYDEQHAKREQEMSLSSGVGYGISGALILGGGIGAGVTAALHRKNQPAEQTITTRTVV
                     DNQPTNNASAQGNTDTSGPEESPASRRNSNASLASNGSDTSSTGTVENPYADVGMPRN
                     DSLARISEEPIYDEVAADPNYSVIQHFSGNSPVTGRLVGTPGQGIQSTYALLASSGGL
                     RLGMGGLTGGGESAVSTANAAPTPGPARFV"
BASE COUNT          469 a          374 c          435 g          395 t
ORIGIN      
        1 tagcaatata aaggattatt atctatttac ataaaataaa aaggagatat tttatgccta
       61 ttggtaatct tggccacaat cccaatgtga gagctttaat tccacctgca ccgccattac
      121 cttcacaaac cgacggtgca ggaggtgccc gtaatcagct cattaactca aatggcccga
      181 tggggtctcg tttgctattt acgcctataa ggaattctgt tgctgatgct gctgattctc
      241 gtgccagtga tattcccgga cttcctacaa atccactgcg ctttgctgcg tccgaggtat
      301 ctttgcatgg tgcgcttgaa gttcttcatg ataaaggggg gcttgatact cttaactctg
      361 ctattggatc ttcgttattc cgtgttgaaa ctcgggatga tggcagccat gttgctatcg
      421 ggcaaaaaaa tggcctcgag accactgttg ttttaagtga gcaagagttt tctagcttac
      481 agtcccttga tcctgaaggt aaaaacaaat ttgtatttac tggaggccgc ggtggcgcag
      541 ggcatgctat ggtcacggtt gcttcagata tcgccgaagc ccgtcagagg ataatagata
      601 aattagaacc aaaggataca aaggagacga aggagccagg ggatccaaat agtggcgagg
      661 gaaaaatcat tgaaattcat acctcaacct caacttctag cctccgtgca gatcctaaac
      721 tttggttgtc attggggact attgctgcag gtctgatagg gatggctgcg acggggattg
      781 cacaggctgt tgcgttgact ccagagccgg atgacccaat cactaccgac cctgatgctg
      841 cagcaaacac agctgaagca gcggcaaaag atcagttaac gaaagaagca ttccagaacc
      901 cagataacca gaaagttaat atcgatgaga acggaaatgc aattccgtcc ggggaactaa
      961 aagatgatgt tgttgcgcaa atagcagaac aagctaaagc ggcgggtgaa caggccagac
     1021 aggaagctat tgaaagtaat tctcaggcgc agcaaaaata tgatgaacag catgctaaac
     1081 gcgaacagga aatgtctctt tcatcggggg ttggctacgg tattagtggt gcgctgattc
     1141 ttggcggggg aattggtgcc ggtgttactg ctgctcttca tcggaaaaac caaccggcag
     1201 aacaaacaat cactacacgt acggtagtcg ataatcagcc tacgaataac gcatctgcgc
     1261 agggcaatac tgacacaagt gggccagaag agtccccggc gagcagacgt aattcgaatg
     1321 ccagcctcgc atcgaacggg tctgacacct ccagcacggg cacggtagag aatccgtatg
     1381 ctgacgttgg aatgcccaga aatgattcac tggctcgcat ttcagaggaa cctatttatg
     1441 atgaggtcgc tgcagatcct aattatagcg tcattcaaca tttttcaggg aacagcccag
     1501 ttaccggaag gttagtggga accccagggc aaggtatcca aagtacttat gcgcttctgg
     1561 caagcagcgg cggattgcgt ttaggtatgg gaggattaac gggggggggc gagagcgcag
     1621 taagtactgc caatgccgca ccaacgccgg gacccgcacg tttcgtttaa ata
//
LOCUS       DAZFWL020000361         1663 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFWL020000361 DAZFWL020000000
VERSION     DAZFWL020000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.367"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..677)
                     /locus_tag="ACHKNP_RS26760"
                     /old_locus_tag="pgaptmp_005352"
     CDS             complement(<1..677)
                     /locus_tag="ACHKNP_RS26760"
                     /old_locus_tag="pgaptmp_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU4707292.1"
                     /translation="MERLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRRVKFS"
     gene            685..1110
                     /gene="tnpA"
                     /locus_tag="ACHKNP_RS26765"
                     /old_locus_tag="pgaptmp_005353"
                     /pseudo
     CDS             685..1110
                     /gene="tnpA"
                     /locus_tag="ACHKNP_RS26765"
                     /old_locus_tag="pgaptmp_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1174..1467)
                     /locus_tag="ACHKNP_RS26770"
                     /old_locus_tag="pgaptmp_005354"
                     /pseudo
     CDS             complement(1174..1467)
                     /locus_tag="ACHKNP_RS26770"
                     /old_locus_tag="pgaptmp_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT          442 a          395 c          362 g          464 t
ORIGIN      
        1 ttgctgaatt tgaccctgcg gcttaactgt cgctgaagtc tcgccagcgt cttctggttt
       61 ttctgaaaac tgtttacagg ctcaaagact gtgccatctg acagcgtggc gagtttagcc
      121 acgccagcat ccagcccgac cattgatgct gaagggtgaa ccggagtgga tacttcactt
      181 tctgtctgaa tactgatgta ccacttaccg caggactggc tgacagtgac atttttcaca
      241 actcccgtga cctgacggct attccggtag cgcatccagc caagtttcgg cagaaaaata
      301 cggctgtttt cctgatcgag cttaacgccc tgcgggtagc ggaatgcatc attctgtccc
      361 cgctttttga atcggggaaa agcagcccga ttctggaaga agtttttgta agcccgctca
      421 aggtctttca gtgactgttg caatggctgt gagggcgaat ctttaagcca ttgcgtttca
      481 gtggcgtttt tccactcaac cagccaggaa gccattttcc cgtaagggat gtatttatta
      541 ccggcctcat gattctcatt ctgacgtgcc agagcacgat tgaaaacgaa acgacatgcg
      601 cctgcgaagc gcctcatttc acgctcctgt tgaccaccgg gtcttaactg gaatttaaat
      661 gcctgtagtc gttccacagg tcaaattata ttttggtctg tgaaaaaaga aaccgatatc
      721 cggcgtggaa ggcattgtgt tttcctgatg catgttcacc tggtctttgt caccaaatac
      781 cgacgccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt ttcaaatgta
      841 tgtgctgatt ttgaagctga actggttgaa atggatggcg aaccagatca cgtccatttg
      901 ttaattagct atcctcccaa actggcgata tccagtctgg taaacagcct caaaggcgta
      961 tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta ctacaaaggc
     1021 gttttgtgga gtcctggcta ttttgccaat agctgcggag gtgcgccaat atccgtcatc
     1081 cgccaataca ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcgc cttatatccc
     1141 cgccctgaag gacggggttt tacggcgcac cagataaacc aggccaagcg tatgctggtt
     1201 gatcaaataa tatcccccaa gcgcagcaat attaatattc acctgcaatg ggtcgatacc
     1261 tggcttaaaa atattgtatt tcttcccttc atccaataag gattccatta actgcaagtg
     1321 cgcatggtta atttctaaga gacgctgtga ttttgcataa tgcacacctt tactttgatt
     1381 ttcactatgc acaattttta aaaaccaagg gttagcaata taataacccc atgtaaaatc
     1441 aataattttc tcaatggctt tttgtggctg gttaaccaga tgattaacaa acgcaccgat
     1501 accaacagcg tagaataatt acataccgca caacaagaat gtatggtgga aggcgtggaa
     1561 gtcattttct ccccgggggc atcgttatat ccataatgag cgatttttga tattaatatc
     1621 tttcaccatc cgctattttc actacttcag aaaacaaact tat
//
LOCUS       DAZFWL020000362         1652 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFWL020000362 DAZFWL020000000
VERSION     DAZFWL020000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.368"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..129)
                     /locus_tag="ACHKNP_RS26775"
                     /old_locus_tag="pgaptmp_005355"
     CDS             complement(<1..129)
                     /locus_tag="ACHKNP_RS26775"
                     /old_locus_tag="pgaptmp_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707293.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKT"
     gene            complement(126..>1652)
                     /locus_tag="ACHKNP_RS26780"
                     /old_locus_tag="pgaptmp_005356"
     CDS             complement(126..>1652)
                     /locus_tag="ACHKNP_RS26780"
                     /old_locus_tag="pgaptmp_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4707294.1"
                     /translation="TSSFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARDRAE
                     RDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDY
                     ESTLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRMLEKHAGANEKISQQRRDLWKA
                     ENQYAVLKEAATKRQLSEQEKSLLTHEKETLEYKRQLAELGDKVEHQKRLNELAQQAA
                     RFEQQQGAKQAAISAQARGLTDRQAQRESEEQRLRDVYGDNPDALAKATSALKNTWSA
                     EEQLRGSWMAGLKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAMLTGSEQNWRG
                     FTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQAAAANFHFATGG
                     FTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSSAQMRR
                     TEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDEIQAQMRDGGVFSGGRR
                     "
BASE COUNT          286 a          573 c          396 g          397 t
ORIGIN      
        1 tgttttcagg tcagcattca gccccgccgc catacgctgc gaatacccgt caccaaaacg
       61 cacttcacgc accgatggct gcgagttcac ctccatatcc ggcttcactt tccagcgaaa
      121 tgttttcatc gcctgcctcc ggaaaagacg ccgccatcac gcatctgcgc ctgaatctca
      181 tcctgcgccc ccttgcgggc catgtcatac accgccttca tcagctgcgg ccccgcctgt
      241 ccgttggtgc cgtcgttctg aatcaccacg tgattgttct gattaaaatt aatgccttcc
      301 gtccgccgca tctgcgccga acttccggca ccgcccacat aaccaccttc cgcatagccc
      361 cgcatcaggc ggtacaggtt gccgacgcca atccggctgg ttgcttcctt cgtgaagaca
      421 aactccccgc gatggacaat accggcaggt tcgtatttgc caccggttcc cgtaaatcct
      481 ccggtcgcga aatggaagtt cgccgccgca gcctgaatgg ccgtccccgt ggaggcagac
      541 gcaccaccac cgaaagcacc gccaatggcg ctgccgatac tcccgactat ccccaccatc
      601 gcctgcttca gaaaaatctc tgtcagcatg gagagcacag aacgggtgaa accacgccag
      661 ttctgttcgc tgccggtcag catcgctgcc atattctgtg caataccgtc aaaggtctgc
      721 gtggccacgc ttttaacctg cgaaaaactg tccgtcgcac tttctgccca ctcgccccag
      781 ccggacttca gaccggccat ccagcttcca cgaagctgct cctccgcaga ccaggtgttc
      841 ttcagtgcag atgtggcctt cgccagcgca tccggattat caccgtacac gtcacgaagg
      901 cgctgctctt ccgactcccg ctgcgcctga cggtcggtga ggccccgcgc ctgggcactg
      961 attgccgcct gcttcgcgcc ctgctgctgc tcaaaccgcg cagcctgctg tgccagctca
     1021 ttcagccgtt tctggtgttc aactttgtct cccagctcag ccagctggcg tttgtactcc
     1081 agcgtctctt tctcatgggt cagcagggat ttttcctgct cagataactg ccgtttcgtg
     1141 gctgcctctt tcaggaccgc atactgattt tccgctttcc ataaatcgcg acgctgctgg
     1201 ctgattttct cattcgctcc ggcatgcttc tccagcatcc ggagttctgc ctgaagcgtc
     1261 agcagggcag catgagcact gtcttcctga cgatcgcccg cagacacctt cacgccggac
     1321 tgtttcggct ttttcagcgt cgattcataa tcctttttcg ccgccgccat cagcgtgttg
     1381 taatctgcct gcaggatttt cccgtctttc agtgccttat tcagttcttc ctgacgggcg
     1441 gtatatttct ccagcggcgt cagcagacgc tcatacgcct tctgcgcctc tccggtatac
     1501 ttcagctgtg atgcgtcccg ttcggcccgg tccctggcgg ccagttcacc ggctttttcc
     1561 atatccgact gcagcgtggc cgctgccagc cccagacggg cattttcccg gtcattccat
     1621 gcgccctgaa ggttggcccg gaaagaggag gt
//
LOCUS       DAZFWL020000363         1646 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFWL020000363 DAZFWL020000000
VERSION     DAZFWL020000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.369"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..488
                     /locus_tag="ACHKNP_RS26785"
                     /old_locus_tag="pgaptmp_005357"
     CDS             <1..488
                     /locus_tag="ACHKNP_RS26785"
                     /old_locus_tag="pgaptmp_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707295.1"
                     /translation="GEDTAPEAVYARLHEQLGEGTIIHIVPRLAGAGKGGLQIVLGAA
                     AIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKAKTPDYRA
                     TDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRQ
                     A"
     gene            734..>1646
                     /locus_tag="ACHKNP_RS26790"
                     /old_locus_tag="pgaptmp_005358"
     CDS             734..>1646
                     /locus_tag="ACHKNP_RS26790"
                     /old_locus_tag="pgaptmp_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707296.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GMQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLVAADVDKWALYAI"
BASE COUNT          392 a          415 c          526 g          313 t
ORIGIN      
        1 gcggtgagga cacggcaccg gaggcggtgt acgcccgtct tcacgaacaa ctgggtgagg
       61 gaacgatcat ccacattgtg ccgcgactgg ccggggccgg aaagggtgga ctgcagattg
      121 tgctgggggc ggcagccatc gtgggctctt tcttcactgc cggggcatca atggcgttat
      181 ggggttcagc cctggcagcc ggtggttttt ctgccaccac gatgctgttt tcactggggg
      241 ccagcatgat actgggtggt gtggctcaga tgcttgcccc gaaggcaaaa acaccggatt
      301 accgcgcaac ggataacggc agacagaaca cgtatttttc ctcactggac aacatgattg
      361 ctcaggggaa cccgatgccg gtgccttacg gtgaaatgct ggttggctca cggcgaatct
      421 cccaggacat cagtacccgt gatgaaggcg gtgacgggaa ggtggtggtt atcgggcggc
      481 aggcataaaa gcgaaaaaat cccgcagtga ccgaaggctg cgggaacaga aaatgaagat
      541 taaccacagg gggttttgtt tttattggcc cgaaaaaact gtaacgcccg ggaatgatat
      601 ctgccacggg ggcgtacaga aaatgtgaag aaattcagaa attttattcc gtcatgacac
      661 aggcaccctc cggggtgcct gtcgtttttg gggcataaac agattcagac atcagacagg
      721 agagggggac cgagtgggta aaggtggcgg cagggcgcac acgccggtag aggcaaagga
      781 caatcttaag tccacgcaga tgatgagcgt gattgacgcc attggtgaag ggccgattga
      841 aggtccggtg aaggggctgc agagtatcct ggtgaacaaa accccgctga cggacacgga
      901 cggtaatcct gtgatacatg gtgtgacagc ggtctggcgc gccggggagc aggagcagac
      961 accgccggaa ggttttgagt catccggctc tgaaaccgca ctgggcgtgg aagtgacgaa
     1021 ggcaaagccg gtgacgcgca ccatcacgtc agcgaacatt gaccgtctgc gggtcacctt
     1081 cggggtgcag tcactggtgg agaccacctc aaagggcgac cgtaatccct cttctgtccg
     1141 actgctgatt cagttgcagc gtaacggtaa ctgggtgacg gaaaaggatg tcaccattaa
     1201 cggcaagacc acctcgcagt ttctggcgtc ggtgattctg gataatctgc ctccccgtcc
     1261 ttttaacatc cggatggtcc gggagacagc ggacagcacc tcggaccagc tgcagaataa
     1321 gacgctctgg tcgtcataca ccgaaatcat cgatgtgaaa cagtgctacc cgaacacggc
     1381 gattgtgggg atgcaggtgg atgcggagca gttcggcggt cagcagatga cggtgaacta
     1441 ccatatccgc ggtcgcatca tccaggtacc gtcaaactat gacccggaaa aacgcacgta
     1501 cagcggcatc tgggacggca gcctgaaacc ggcatacagc aacaacccgg cctggtgcct
     1561 gtgggacatg ctgactcacc cgcgctacgg catgggaaaa cgtctggtgg cggcggatgt
     1621 tgacaagtgg gcgctgtatg ccatcg
//
LOCUS       DAZFWL020000364         1634 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFWL020000364 DAZFWL020000000
VERSION     DAZFWL020000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.370"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..98
                     /locus_tag="ACHKNP_RS26795"
                     /old_locus_tag="pgaptmp_005359"
     CDS             <1..98
                     /locus_tag="ACHKNP_RS26795"
                     /old_locus_tag="pgaptmp_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707297.1"
                     /translation="YEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            304..1587
                     /gene="gltA"
                     /locus_tag="ACHKNP_RS26800"
                     /old_locus_tag="pgaptmp_005360"
     CDS             304..1587
                     /gene="gltA"
                     /locus_tag="ACHKNP_RS26800"
                     /old_locus_tag="pgaptmp_005360"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HGU4707298.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          414 a          401 c          390 g          429 t
ORIGIN      
        1 gttatgaagt cggtatttca cctaagatta acttatgtaa cagtgtggaa gtattgacca
       61 attcattcgg gacagttatt agtggtagac aagtttaata attcggattg ctaagtactt
      121 gattcgccat ttattcgtca tcaatggatc ctttacctgc aagcgcccag agctctgtac
      181 ccaggttttc ccctctttca cagagcggcg agccaaataa caaacgggta aagccaggtt
      241 gatgtgcgaa ggcaaattta agttccggca gtcttacgta ataaggcgct aaggagacct
      301 taaatggctg atacaaaagc aaaactcacc ctcaacgggg acacagctgt tgaactggat
      361 gtgctgaaag gcacgctggg tcaagatgtt attgatatcc gtactctcgg ttcaaaaggt
      421 gtgttcacct ttgacccagg cttcacttca accgcatcct gcgaatctaa aattactttt
      481 attgatggtg atgaaggtat tttgctgcac cgcggtttcc cgatcgatca gctggcgacc
      541 gattctaact acctggaagt ttgttacatc ctgctgaatg gtgaaaaacc gactcaggaa
      601 cagtatgacg aatttaaaac tacggtgacc cgtcatacca tgatccacga gcagattacc
      661 cgtctgttcc acgctttccg tcgcgactca catccaatgg cagtcatgtg tggtattacc
      721 ggcgcgctgg cggcgttcta tcacgactcg ctggatgtta acaatcctcg tcatcgtgaa
      781 attgccgcgt tccgcctgct gtcgaaaatg ccgaccatgg ccgcgatgtg ttacaagtat
      841 tccattggtc agccatttgt ttatccgcgc aacgatctct cctatgccgg taacttcctg
      901 aatatgatgt tctccacgcc gtgcgaaccg tatgaagtta atccgattct ggaacgtgct
      961 atggaccgta ttctgatcct gcacgctgac catgaacaga acgcctctac ctccaccgtg
     1021 cgtaccgctg gctcttcggg tgcgaacccg tttgcctgta tcgcagcagg tattgcttca
     1081 ctgtggggac ctgcgcacgg tggtgctaac gaagcggcgc tgaaaatgct ggaagaaatt
     1141 agctccgtta aacacattcc ggaatttgtt cgtcgtgcga aagataaaaa tgattctttc
     1201 cgcctgatgg gcttcggtca ccgcgtgtac aaaaattacg acccgcgcgc caccgtaatg
     1261 cgtgaaacct gccatgaagt tctgaaagag ctgggcacca aagatgacct gctggaagtg
     1321 gctatggagc tggaaaacat cgcgctgaac gacccgtact ttatcgagaa gaaactgtac
     1381 ccgaacgtcg atttctactc tggtatcatc ctgaaagcga tgggtattcc gtcttccatg
     1441 ttcaccgtca ttttcgcaat ggcacgtacc gttggctgga tcgcccactg gagcgaaatg
     1501 cacagtgacg gtatgaagat tgcccgtccg cgtcagctgt atacaggata tgaaaaacgc
     1561 gactttaaaa gcgatatcaa gcgttaatgg ttgattgcta agttgtaaat attttaaccc
     1621 gccgttcaaa tggc
//
LOCUS       DAZFWL020000365         1617 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFWL020000365 DAZFWL020000000
VERSION     DAZFWL020000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.371"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..202
                     /locus_tag="ACHKNP_RS26805"
                     /old_locus_tag="pgaptmp_005361"
                     /pseudo
     CDS             1..202
                     /locus_tag="ACHKNP_RS26805"
                     /old_locus_tag="pgaptmp_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807328.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            331..576
                     /locus_tag="ACHKNP_RS26810"
                     /old_locus_tag="pgaptmp_005362"
     CDS             331..576
                     /locus_tag="ACHKNP_RS26810"
                     /old_locus_tag="pgaptmp_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707299.1"
                     /translation="MDIRHSLNEWWTLWQASRAAIELDIDWPESNDDFWKDGEEGAYA
                     MGYEDGRDKTVIAVMKAIRAAGIKEKNFDEANNIPPN"
     gene            548..958
                     /locus_tag="ACHKNP_RS26815"
                     /old_locus_tag="pgaptmp_005363"
     CDS             548..958
                     /locus_tag="ACHKNP_RS26815"
                     /old_locus_tag="pgaptmp_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGU4707300.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCDKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            955..1131
                     /locus_tag="ACHKNP_RS26820"
                     /old_locus_tag="pgaptmp_005364"
     CDS             955..1131
                     /locus_tag="ACHKNP_RS26820"
                     /old_locus_tag="pgaptmp_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU4707301.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            1134..1535
                     /locus_tag="ACHKNP_RS26825"
                     /old_locus_tag="pgaptmp_005365"
     CDS             1134..1535
                     /locus_tag="ACHKNP_RS26825"
                     /old_locus_tag="pgaptmp_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707302.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANMTYMKAYQKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            1495..>1617
                     /locus_tag="ACHKNP_RS26830"
                     /old_locus_tag="pgaptmp_005366"
     CDS             1495..>1617
                     /locus_tag="ACHKNP_RS26830"
                     /old_locus_tag="pgaptmp_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HGU4707303.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHV"
BASE COUNT          511 a          328 c          404 g          374 t
ORIGIN      
        1 tgagtttatg ctcgtcgcac tcaaatgcgt tggcgtttga tggattcttc tgacgttatt
       61 tattgttctg catagctaca ttcgtcttgt gaatgacggt aaagacccat ggtatacgtt
      121 gtttggcgct gcatttgtct gggtgattat cggtgttgcg cctgtcgctg tagcaaaaat
      181 ggcgtggcgt tttgtgagtt gaactgaggg taagtatcga tggacgaatc aagaaagcag
      241 tttgaggaat acgttgccaa aaaattgaga ttaccattcg agatgataac cgaggcaaga
      301 aatggtgata ggtacttcgc attttcaagc atggatattc gtcactcctt aaatgagtgg
      361 tggactttat ggcaggcatc gcgagcagct attgaactgg atatcgactg gccagaatcg
      421 aatgacgact tttggaaaga tggtgaagaa ggtgcttatg cgatgggtta tgaggatggg
      481 cgtgacaaaa cggtaattgc agtaatgaaa gctatcagag ccgctggaat taaagagaag
      541 aatttcgatg aagcaaacaa tattcctccg aactaagcaa caacagcaag ccgcaatcaa
      601 cgccatcctc gcaacaccac tcgataaaga caagccagtc accatccgca ttactgacta
      661 caagcgcaac cttgaccaga acgcaaaatt tcacgcgatg ctggcggata tcgcacgtca
      721 ggttcaatgg tgcgataagt ggttaaaacc agaacaatgg aaggttttgt tgattagcgg
      781 tcatgcagtg gcaacaaagc aggaagctga tgttttgccc ggccttgaag gcgaatacgt
      841 caacattcgc gaaagtagcg cgcagatgag tgtgaagcgt atggcaagtc tgattgagta
      901 cacgacagca tgggctattg gtcagggcgt cagatttacc gacaggaggt acgaatgaga
      961 cgacagcgac gaagtatcac cgacataatc tgtgaaaact gcaaatacct tccaacgaaa
     1021 cgctccagaa ataaacgcaa gccaatccca aaagaatctg acgtaaaaac cttcaactac
     1081 acggctcacc tgtgggatat ccggtggcta agacatcgtg cgaggaaatg acaatgcttt
     1141 taattcaacc tggatttgga cttagcatca aaaaaggcca catgtttggt gagaaagagt
     1201 cacaacgaaa aatggtgtct atccggttgc catttatcag tatttattgg ctaaacaggg
     1261 aggcaacaaa ttattggtat acatgcgcca gagcagcatt taacgaccct gactggtttg
     1321 tgaaaaacca ccacgcagtt cgtcaggcaa agagaaaggc caatatgaca tacatgaagg
     1381 cgtatcaaaa agcatggaaa gaacaccgcg atcgatacca acaagacatg gaaaagcttg
     1441 aatcagaaaa catggaatta agacgaaagc tcggtgaagc aaaacgagac attgatgctt
     1501 acaagcgact ttttaatggt gaaagccatg cttagcccat cccaatccct tcaataccag
     1561 aaagaaagcg tcgagcgagc tttaacgtgc gctaactgcg gtcagaagct gcatgtg
//
LOCUS       DAZFWL020000366         1607 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFWL020000366 DAZFWL020000000
VERSION     DAZFWL020000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.372"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(158..247)
                     /locus_tag="ACHKNP_RS26835"
                     /old_locus_tag="pgaptmp_005367"
     CDS             complement(158..247)
                     /locus_tag="ACHKNP_RS26835"
                     /old_locus_tag="pgaptmp_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707304.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(302..514)
                     /gene="cspF"
                     /locus_tag="ACHKNP_RS26840"
                     /old_locus_tag="pgaptmp_005368"
     CDS             complement(302..514)
                     /gene="cspF"
                     /locus_tag="ACHKNP_RS26840"
                     /old_locus_tag="pgaptmp_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HGU4707305.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            815..1030
                     /gene="cspB"
                     /locus_tag="ACHKNP_RS26845"
                     /old_locus_tag="pgaptmp_005369"
     CDS             815..1030
                     /gene="cspB"
                     /locus_tag="ACHKNP_RS26845"
                     /old_locus_tag="pgaptmp_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HGU4707306.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIERGAKGPAAANVIITD"
     gene            complement(1394..1564)
                     /gene="ynfU"
                     /locus_tag="ACHKNP_RS26850"
                     /old_locus_tag="pgaptmp_005370"
     CDS             complement(1394..1564)
                     /gene="ynfU"
                     /locus_tag="ACHKNP_RS26850"
                     /old_locus_tag="pgaptmp_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HGU4707307.1"
                     /translation="MSERKDSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKAVA"
BASE COUNT          487 a          282 c          342 g          496 t
ORIGIN      
        1 gcgggttttg tgctttaaat ggggcaatgg taatgttgaa tctcatcccg ggactcatgt
       61 ctgttaactt attatttagc tggtgacttg gttatttgcc tgatgtttaa aatgttttct
      121 tccagtacaa tgtccctaaa cacaatgagt ctgcttatta tattattagc agagctatta
      181 cggccaaagt acagcataag cttttaaagc caatcaacca gtcatcaaga cagacggggt
      241 tattcataaa aactctccat gtgtgatccg atggggcctg aaattaaagc tttaatatag
      301 ctcatgaaag gtaaacattg gcagctgaag ggccacgcag accatttatc cggcaaaatt
      361 ccacgcgtaa tccggtggta atttcttctg catcgcggag attgagcgct gaaacatgaa
      421 gctggacatc gatacgacca tcggatgggg tgataagacc cttgccgctt ttgccgtcaa
      481 aggttttgac aattcctgtc attttacggg acaaaaaaat tccttaatac tgataacttg
      541 gcgcactata cacacgttcc tgaagaaagc tatagttttt tgatggggtt gaagatggct
      601 ggatgtctaa aataaacatt gcttcatatg ttcaactatg cgttaatgat tgcgtcggtt
      661 tgaagaacag acgatatacg aagtagttta ctaaagcagt tctcatttca ggtgttattc
      721 acttattcct tctttgagtc tctccaatta agtacgaagt cgtttctgtt atgcaaacca
      781 tttatgccga aaggctcaag ttaaggaatg tagaatgtca aataaaatga ctggtttagt
      841 aaaatggttt aacgctgata aaggtttcgg ctttatttct cctgttgatg gtagtaaaga
      901 tgtgtttgtg catttttctg cgattcagaa tgataattat cgaaccttat ttgaaggtca
      961 aaaggttacc ttctctatag agagaggtgc taaaggtcct gcagcagcaa atgtcatcat
     1021 tactgattaa aattcatcgc tcgtctgtat acgataacga agaaggctga tgcctgagta
     1081 gagatacgga cagagtagtg aatattggat ctctttaata aaaagtaagg aggtccaata
     1141 catgaaacaa tggctagcat atttggcaaa atcttaatca ggaaaagtat gctaaccatt
     1201 gtggtgaagt gcaggtttgc tgcatgaata gtttcacagc agaagctaac tgctggcatc
     1261 gcaaaacaaa gtgcgtaagt ggatgactcc cacaaaaagc accacaatct caaacccgct
     1321 caggcgggtt ttttattatc tgctttaaat atattattaa aatataaaaa atacttgtta
     1381 ctaataaaat caatcaggct acagctttaa gatttgtctg gaatactttg ttgcaatgag
     1441 ggcagatcaa aagggcacct ttttgtactc ttgaaaaact gtgttctgac tcttgggtgc
     1501 agtttgggca ggaacattta acgagataat tacggcgtga ttttgagtct ttacgttctg
     1561 acataggctt ttcctgtata atggccgtat acagtacact aaatatg
//
LOCUS       DAZFWL020000367         1579 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFWL020000367 DAZFWL020000000
VERSION     DAZFWL020000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.373"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1579
                     /locus_tag="ACHKNP_RS26855"
                     /old_locus_tag="pgaptmp_005371"
     CDS             <1..>1579
                     /locus_tag="ACHKNP_RS26855"
                     /old_locus_tag="pgaptmp_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGU4707308.1"
                     /translation="ASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAAAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAETPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVS"
BASE COUNT          439 a          467 c          457 g          216 t
ORIGIN      
        1 acgcgtccgc ggtggcacag aacacggcag ccgcgaagaa gtcagccagc gatgccagca
       61 catcagcccg tgaggcggca acccatgcga ctgatgctgc aggctcagca cgcgcagcca
      121 gcacgtcagc cggacaggcc gcgtcgtcgg ctcagtcagc gtcttccagc gcaggaacgg
      181 catcaacaaa ggccactgaa gcatcaaaaa gtgctgccgc tgcagagtcc tcaaaaagcg
      241 cggcggccac cagtgccgct gcggcgaaaa cgtcagaaac gaatgcggca gcgtcacaac
      301 aatcagcagc cacttctgca tccaccgcga ccacgaaagc gtcagaagct gccacctcag
      361 cccgggatgc gtcggcttca aaagaagcgg caaaatcatc agaaacgaac gcatcatcaa
      421 gcgccagtag tgccgcttcc tcggcaacgg cggcaggaaa ttccgcgaag gcggcaaaaa
      481 cgtccgagac gaacgctaag tcttctgaga cggcagcggg acagagcgcc tcagctgcgg
      541 caggctcaaa aacagcggct gcgtcgtctg ccagtgccgc gtcaacaagt gccgggcagg
      601 cctcagccag tgccaccgcc gccggaaaat cggcagaaag cgccgcatca tccgcttcaa
      661 cagccacaac gaaggctggc gaagccactg aacaggccag cgcagcagcg agttctgctt
      721 ccgcagcgaa gacatccgaa acgaacgcga aagcgtcgga aaccagcgca gaatcctcaa
      781 aaacggctgc cgcatcgtca gccagttcgg cggcgtcatc ggcatcatcg gcgtctgctt
      841 caaaagatga ggcgaccaga caggcgtcag cagcgaagag cagcgccacg acggcatcca
      901 cgaaggcgac agaggcagct ggcagtgcga cggcggcagc tcagagcaaa agtacggcgg
      961 aatccgcggc aacgcgcgcc gagacagcag ctaaacgggc agaggatatt gcatccgccg
     1021 tggcgcttga ggatgcgagc acgacgaaaa aggggatagt acagctcagc agtgcgacta
     1081 acagcacttc cgagtcactg gcggcaacgc caaaagccgt taaggccgcg tatgacctgg
     1141 ctaacgggaa atacaccgca caggatgcaa cgacagcaca gaaagggatc gttcagctca
     1201 gcagcgcgac caacagcaca tctgaaatgc tggcggcaac gccaaagtcg gtaaaggcag
     1261 cctatgacct tgctaacggg aaatataccg ctcaggacgc tacgacagca caaaaaggaa
     1321 ttgtccagct cagtagtgca accaacagcg catctgaaac gcttgccgag acaccgaaag
     1381 cagtgaaagc agctaatgat aatgcgaatg gtcgggtacc ttctgcccgt aaggtgaatg
     1441 gtaaggcgct ttcagcggat ataacactga cgccgaaaga tattggtacg cttaactcaa
     1501 caacaatgtc attcagcggt ggtgctggtt ggttcaaatt agcaacggta accatgccac
     1561 aggcgagttc tgttgtttc
//
LOCUS       DAZFWL020000368         1563 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFWL020000368 DAZFWL020000000
VERSION     DAZFWL020000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.374"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..615
                     /locus_tag="ACHKNP_RS26860"
                     /old_locus_tag="pgaptmp_005372"
     CDS             <1..615
                     /locus_tag="ACHKNP_RS26860"
                     /old_locus_tag="pgaptmp_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4707309.1"
                     /translation="TRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIILLNSHDGTSSY
                     QMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQ
                     SLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENML
                     KGGISGRSAKGKRIHTRAIHSIDTDIKLNRVLWVMAETLLESLR"
     gene            670..1155
                     /locus_tag="ACHKNP_RS26865"
                     /old_locus_tag="pgaptmp_005373"
     CDS             670..1155
                     /locus_tag="ACHKNP_RS26865"
                     /old_locus_tag="pgaptmp_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4707310.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            1171..>1563
                     /locus_tag="ACHKNP_RS26870"
                     /old_locus_tag="pgaptmp_005374"
     CDS             1171..>1563
                     /locus_tag="ACHKNP_RS26870"
                     /old_locus_tag="pgaptmp_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HGU4707311.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQAL"
BASE COUNT          358 a          441 c          432 g          332 t
ORIGIN      
        1 acccgtgtgc gcgacccggg ccgccgggga tacacaaaac acatgctgcg tctgcggcgg
       61 gccggagaga taaacggaga acatgtccct gaaattattc tgctcaactc tcatgacggt
      121 acctccagct accagatgct gccgggttac ttcaggttcg tctgccagaa cggatgcgtc
      181 tgtggtcagt ctctggggga agtgcgtgtt ccgcaccggg gaaatgtggt ggacagagtc
      241 attgaagggg cttacgaggt ggtgggcgtg tttgaccgga ttgaggaaaa gcgtgatgcc
      301 atgcagtcgc tgattctgcc gccaccggca cgccaggcgc tggcacaggc ggcactgact
      361 taccgttatg gtgacgaaca tcagcccgtc accaccgccg acattctgac gccacgacgc
      421 cgggaggatt acggtaagga cctgtggagt gcttatcaga ccattcagga gaatatgctg
      481 aaaggcggaa tttcaggtcg cagcgctaaa ggaaaacgta tccatacccg tgccattcac
      541 agcattgaca ctgacattaa gctcaaccgc gtattgtggg tgatggcgga aaccctgctg
      601 gagagcctgc gctgattcag ccattaccct gaaaaccacg catttatttg tcctttaagg
      661 agtcatttca tgaaaaccat ttcacataat tccaccacat cttctgtttc cgtaaccgct
      721 gcatcaggga atgaccagcc tcagttggtt gccaccctcg tccctgatga acagcgcatc
      781 agcttctggc cgcagcactt tggcctcatt ccacagtggg tgaccctgga gccccgtgtc
      841 ttcggctgga tggaccgtct gtgtgaagac tactgcggtg gtatctggaa tctgtacacc
      901 ctgaacaacg gcggggcatt tatggcaccc gaaccggatg acgatgatga cgaaacatgg
      961 gtactgttca atgccatgaa cggtaaccgc gctgaaatga gtccggaagc tgccggcatt
     1021 gctgcctgtc tgatgacgta cagtcatcat gcctgtcgta cggagtgtta tgccatgacg
     1081 gtccattatt accggctgcg ggattacgcc ctgcagcatc cggaatgcag cgccattatg
     1141 cgcatcatcg actgaatgga gagaagtaca atgcaacagc tttcctttct gcccggagag
     1201 atgacgcccg gcgagcgcag cctcattcaa cgggccctga aaaccctgga ccgccatctt
     1261 catgaacccg gcgtggcctt cacctccacc cgtgcggcac gagaatggct gattctgaac
     1321 atggcgggac tggagcgtga agagttccgg gtgctgtatc tgaacaacca gaatcagctg
     1381 attgccggtg aaaccctctt caccggcacc atcaaccgca cggaagtcca tccccgtgaa
     1441 gtgattaaac gcgccctgta tcacaatgcc gctgctgtgg tgctggcaca caatcacccg
     1501 tccggtgaag tcacacccag caaggcagac cgccttatca cggaacgtct ggtacaggca
     1561 ctg
//
LOCUS       DAZFWL020000369         1563 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFWL020000369 DAZFWL020000000
VERSION     DAZFWL020000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.375"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            102..773
                     /gene="queE"
                     /locus_tag="ACHKNP_RS26875"
                     /old_locus_tag="pgaptmp_005375"
     CDS             102..773
                     /gene="queE"
                     /locus_tag="ACHKNP_RS26875"
                     /old_locus_tag="pgaptmp_005375"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HGU4707312.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     repeat_region   1117..1446
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1117..1141
                     /rpt_unit_seq="tccccgcgccagcggggataaaccg"
BASE COUNT          404 a          360 c          381 g          418 t
ORIGIN      
        1 gcatttttct ttaaatctgc ctgtaacaga atgttaataa gacatgattg ttgtggcagg
       61 atctgcaata attaacaatt atccacttca cagagaatgc tatgcagtac ccgattaacg
      121 agatgttcca gaccctgcaa ggtgagggtt actttaccgg cgtccccgcc atttttattc
      181 gtttacaggg atgcccggtt ggctgtgcct ggtgcgacac caaacacacc tgggaaaagc
      241 ttgaggatcg ggaagtctcc cttttcagca ttctggcgaa gaccaaagag agtgataagt
      301 ggggggcggc gagcagtgaa gatttgctgg ctgtcattgg tcggcaggga tataccgcgc
      361 ggcatgtggt gattacgggt ggtgagcctt gcattcatga tttgctgcca ctgactgacc
      421 tgctcgaaaa gaacggtttt agctgccaga tcgaaaccag tggaactcat gaagtacgct
      481 gcacaccgaa tacctgggtt accgtatcgc caaagctgaa catgcgcggc ggctatgacg
      541 tgttgtcaca ggcactggag cgagccaacg aaatcaagca tccagtgggg cgcgtacgtg
      601 atattgaagc actggatgaa ctactggcaa cgctgaccga tgataaaccg cgagtcattg
      661 cactgcagcc gattagccaa aaggatgatg ccacacgttt gtgcattgaa acctgcattg
      721 cgcgtaattg gcgtttgtcg atgcaaacac ataaatatct aaatattgcc tgattaaaca
      781 tttataagcg ttataaatgg gtggaacctg taaagacttc tactcattta tattgtttgt
      841 cgcctctgaa aactcctcca ttttacccat ccagggctaa tcattagcat tctctacaaa
      901 ttctgtggca ttaatttttc gctggagtga aaattattgc ggtaaagttt ggtagatttt
      961 agtttgtata gagttatttt aaatatttac ctttttaatc aatggattaa gtgctcttta
     1021 acataatgga tgtgttgttt gtgtgatact ataaagttgg tagattgtga ctggcttaaa
     1081 aaatcattaa ttaataatag gttatgttta gagtgttccc cgcgccagcg gggataaacc
     1141 gatctcctca tcctcccagc taccgacagt agtgtgttcc ccgcgccagc ggggataaac
     1201 cgtaaggccg tcgccggatc agcctggcta tgccgagctc cccgcgccag cggggataaa
     1261 ccgttcttgc gggtgttgca aatattcttc acgtagagct ccccgcgcca gcggggataa
     1321 accggagcct gacgagacta ctgaggccgt tctgtcgagt tccccgcgcc agcggggata
     1381 aaccggacgc cgccgccgcg aagccgtttc cgatgttgag ctccccgcgc cagcggggat
     1441 aaaccaccat ataacccgtt atctctttct caagttttta tattagcagt acttgtaata
     1501 agcaacatat ccacgtaaca cctcatgttc aaaatagttc tccatgccag agaggttcac
     1561 aat
//
LOCUS       DAZFWL020000370         1551 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFWL020000370 DAZFWL020000000
VERSION     DAZFWL020000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.376"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1551
                     /locus_tag="ACHKNP_RS26880"
                     /old_locus_tag="pgaptmp_005376"
     CDS             <1..>1551
                     /locus_tag="ACHKNP_RS26880"
                     /old_locus_tag="pgaptmp_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4707313.1"
                     /translation="VYDRSGKQVRSFTYDDKYRGRMVAHRHTGRPEICYRYDSDGRVT
                     EQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFD
                     AVGRLKAQTDAAGRTTEYSPDVVTGLTTPDGRASAFYYNHHNQLTSATGPDGLELRRE
                     YDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCS
                     GYVTRYDHDRFGQVTAVHREEGLSQYHAYDSRGQLTAVKDTQGHETRYEYNIAGDLTA
                     VIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLD
                     RLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWYYDEADRLTHRTVKGETAERWQY
                     DERGWLTDISHISEGHRVAVHYGYDSKGRLASEHLTVHHPQTEALLWQHETRHAYNAQ
                     GLAKRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVEYTRDRLHRETLRRFGRYELTTA
                     YTPAGQLQR"
BASE COUNT          381 a          456 c          470 g          244 t
ORIGIN      
        1 ggtgtatgac cgtagtggca aacaggtgcg cagctttact tacgatgata aataccgggg
       61 ccggatggtg gcgcaccgtc acacgggccg accggaaatc tgttaccgtt acgacagcga
      121 cgggcgggtg acagaacagc taaacccggc aggcttaagt tacacgtatc agtatgaaaa
      181 agaccgcatc accatcaccg acagcctgaa ccgccgtgaa gtgctgcaca cgcagggcga
      241 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
      301 gtttgacgcg gtgggcaggc ttaaggcaca gacggatgcc gcaggccgga caacagaata
      361 cagcccggat gtggtgacgg gcctcaccac gccggatggc agggcatcgg cgttttacta
      421 taaccaccac aaccagttaa cgtcagccac cgggcctgac gggctggaat tgcgccggga
      481 atatgatgaa tccggccgtc tgattcagga aactgcccct gacggcgata tcacccgcta
      541 ccgttatgat aatccacaca gtgacttacc ctgcgcaacg gaagatgcca ccggcagccg
      601 gaaaaccatg acgtggagcc gttacggtca gttgctgagc ttcaccgact gttccggtta
      661 tgtaacccgc tatgaccatg accgttttgg tcaggtgacg gcggtgcacc gcgaggaagg
      721 cctgagccag taccacgcat acgacagccg tggacagtta actgcggtga aagacacgca
      781 gggccatgaa acgcggtatg aatacaacat cgccggtgac ctgaccgccg tcattgcccc
      841 ggacggcagc agaaacggga cacagtacga tgcgtgggga aaggccatct gtaccacgca
      901 gggcgggctg acgcgcagta tggaatacga tgctgccgga cgggtcatcc gcctgaccag
      961 tgaaaacggc agccacacca ccttccgtta cgatgtactc gaccggctga tacaggaaac
     1021 cggctttgac ggccgcacac agcgttatca ccacgacctg accggcaaac ttatccgcag
     1081 cgaggatgag gggctggtca cccactggta ctatgacgaa gcagaccgcc tcactcaccg
     1141 caccgtgaag ggtgaaaccg cagagcggtg gcagtatgac gaacgcggct ggctgacaga
     1201 catcagccat atcagcgaag ggcaccgggt ggcggtgcac tatggttatg acagtaaagg
     1261 ccgcctcgcc agtgaacacc tgacggtgca tcatccgcag acggaagcac tgctctggca
     1321 gcatgagacc agacatgcgt acaacgcgca gggactggcg aaacgctgta taccggacag
     1381 cctgcccgcc gtggaatggc tgacctacgg cagcggttac ctggcaggca tgaaactcgg
     1441 cgacacaccg ctggtggagt acacccgcga ccgcctgcac cgggaaacgc tgcgccgctt
     1501 cggccgttat gaactcacca ccgcttatac ccctgccggg cagttacagc g
//
LOCUS       DAZFWL020000371         1545 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFWL020000371 DAZFWL020000000
VERSION     DAZFWL020000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.377"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..357)
                     /locus_tag="ACHKNP_RS26885"
                     /old_locus_tag="pgaptmp_005377"
     CDS             complement(<1..357)
                     /locus_tag="ACHKNP_RS26885"
                     /old_locus_tag="pgaptmp_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4707314.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVP"
     gene            485..1345
                     /locus_tag="ACHKNP_RS26890"
                     /old_locus_tag="pgaptmp_005378"
     CDS             485..1345
                     /locus_tag="ACHKNP_RS26890"
                     /old_locus_tag="pgaptmp_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4707315.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNMKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQIEDIISVINWAEKQACIDNQRIG
                     LWGTSLGGCHVFSAAAQDQRVKCIVSQLAFADGDALITGEMNELERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLNDDESKVFFEKVKAQHPEMDIKIPFLTVMETLQYKPAESAA
                     RVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGTFFERVAA
                     VQTQWFKKYI"
BASE COUNT          420 a          304 c          405 g          416 t
ORIGIN      
        1 cggaaccaga cggacttcat cttttggcgg gcgtccgcgt cgcgcgcgca ctttttcgga
       61 caggatccgc acaaacggtc ttacaccatc cagatcaaat gattccggtg tcagcatccg
      121 ggcttcgcca atacgcattc cggtattcca gagggtggcg aacagcatat ggtgacgctg
      181 atccggcata tagaaaagaa gggcactcac ttccggggcc agcaggtagg ccggtgtggc
      241 ccctgtggaa gtggccattc ttctcaggga gagcgctgtt gcaaaatcca cccccggcgc
      301 aatgggtaac agggagacgc gttctggtga attctgcagg ggaatgacat tgttcatgag
      361 ttatttttca cgtagcacac gcactgttta tggatactga ttgcgctggg cattctggta
      421 ttgtgactgg tatgatgctg gcgctgattc tgatgattaa taaacaacat aacacaggat
      481 atttatgaaa attacggatc acaaactttc tgaaggtatt gctctgacat ttcgtgttcc
      541 tgaaggaaat atgaaacatc ctttgattat cttatgccat ggattttgcg gtatccggaa
      601 tgttctgctc ccttgctttg cgaatgcttt cacagaagca gggttcgcaa cgattacttt
      661 tgattatcgt ggattcggtg agagtgaagg agaacggggc cgtctggttc cagcaatgca
      721 gatcgaagat attatttcag ttatcaattg ggcagaaaag caggcatgta ttgataatca
      781 gcgtatcggg ttatggggaa catcgctggg tggttgtcat gtgttcagtg cagctgcgca
      841 agatcaaaga gtgaaatgta ttgtcagcca gctggctttt gctgatggtg atgctctgat
      901 taccggagag atgaatgaat tggagagagc ttcttttctg tccacactga ataaaatggc
      961 agagaaaaag aaaaacacag ggaaggagat gtttgtgggg gttacacggg tactaaatga
     1021 tgatgagtcg aaggtattct tcgagaaagt caaagctcag catcccgaaa tggatataaa
     1081 aatacctttc cttactgtca tggaaacgtt gcaatataaa ccggccgaga gcgcagccag
     1141 agtccagtgt ccagtattgg ttgttattgc tggccaggat agtgttaatc caccggaaca
     1201 gggaagagca ttatatgatg ctgttgcttc tggtactaaa gaattatatg aggaagctga
     1261 cgcatgccat tatgatattt atgagggcac attttttgag cgggtagcag ctgtgcaaac
     1321 acaatggttt aaaaaatata tttaatagtg agcctgtgtt taccattctg tgtaaatact
     1381 ttttcttaaa agtgaccgtc aaggcggtca ctaaacttta taataaagag gcatcgttca
     1441 tttccgggag gcagactgga ttgccagccc cccacttttt accaagctgc aggtatgggg
     1501 gacacggaca ctggtgttca cagaaaaaaa taaccatctc tgaaa
//
LOCUS       DAZFWL020000372         1545 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFWL020000372 DAZFWL020000000
VERSION     DAZFWL020000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.378"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..722
                     /locus_tag="ACHKNP_RS26895"
                     /old_locus_tag="pgaptmp_005379"
     CDS             <1..722
                     /locus_tag="ACHKNP_RS26895"
                     /old_locus_tag="pgaptmp_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU4707316.1"
                     /translation="GHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVA
                     LGTRHSMKASTDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGK
                     DNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVSELPVNWWVQ
                     PSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYA
                     HSVSGSSAEGYNGQATLNVTF"
     gene            843..>1545
                     /locus_tag="ACHKNP_RS26900"
                     /old_locus_tag="pgaptmp_005380"
     CDS             843..>1545
                     /locus_tag="ACHKNP_RS26900"
                     /old_locus_tag="pgaptmp_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YeeR"
                     /protein_id="HGU4707317.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLT"
BASE COUNT          332 a          425 c          427 g          361 t
ORIGIN      
        1 ctggccattc ttccgttgat gttaaggatg atgacggctc ccgtgccggc acggtccggg
       61 atgatgccgg cagcctgggc ggatacctga atctgataca caacgcctcc ggactgtggg
      121 ctgacattgt ggccctggga acccgccaca gcatgaaagc gtcaacggac aataacgact
      181 tccgcgcccg gggttggggc tggctgggtt cactggaaac cggtctgccc ttcagtatca
      241 ctgacaacct gatgctggag ccacaactgc agtatacctg gcagggactt tccctggatg
      301 acggtaagga caacgccggt tatgtgaagt tcgggcatgg cagtgcacaa catgtgcgtg
      361 ccggtttccg tctgggcagc cacaacgata tgacctttgg cgaaggcacc tcatcccgtg
      421 cccccctgcg tgacagtgca aaacacagtg tgagtgaatt accggtgaac tggtgggtac
      481 agccttctgt tatccgcacc ttcagctccc ggggagatat gcgtgtgggg acttccactg
      541 caggcagcgg gatgacgttc tctccctcac agaatggcac atcactggac ctgcaggccg
      601 gactggaagc ccgtgtccgg gaaaatatca ccctgggcgt tcaggccggt tatgcccaca
      661 gcgtcagcgg cagcagcgct gaagggtata acggtcaggc cacactgaat gtgaccttct
      721 gacagaacca tcgcctctct gtggtcccgg tcatcatgac cgggacccgg accggcgcaa
      781 cggatcttca acgccacatt cgctggcatt aacaataaca tgatattcat cacggagtga
      841 ctatgttaca gatagtcggc gcgctgatcc tgctgatcgc aggatttgcc attcttcgcc
      901 ttttgttcag agcattaatc agcacggctt ctgcgctggc agggctcata ttgctgtgtc
      961 tgttcggccc ggccttactg gctggctata tcaccgaacg cataacccgg ttgttccata
     1021 ttcgctggct ggcaggcgta tttctgacga ttgccggaat gatcatcagc ttcatgtggg
     1081 gacttgatgg taaacatatc gcgctggagg ctcacacctt tgactctgtg aaatttattc
     1141 tgaccaccgc tctcgccggt ggtctgctgg ctgttcccct gcagatcaaa aacattcagc
     1201 agaacgggat cacaccagaa gatatcagca aggaaattaa cgggtattac tgctgttttt
     1261 atactgcctt tttccttatg gcgtgttctg catgcgcacc attgatcgcg ttacagtacg
     1321 atatttcacc gtcactgatg tggtggggcg ggttgttgta ctggctggct gcattagtga
     1381 cgctgctatg ggcggccagc cagatccagg cgctgaaaaa actgacctgt gccatcagcc
     1441 agacactgga agaacaaccg gtgctcaaca gtaaatcgtg gctgaccagt ttgcaaaacg
     1501 attacagcct tcctgactca ctgacggagc gcatctggct gacgc
//
LOCUS       DAZFWL020000373         1517 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFWL020000373 DAZFWL020000000
VERSION     DAZFWL020000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.379"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1412)
                     /locus_tag="ACHKNP_RS26905"
                     /old_locus_tag="pgaptmp_005381"
     CDS             complement(<1..1412)
                     /locus_tag="ACHKNP_RS26905"
                     /old_locus_tag="pgaptmp_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4707318.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFDGTPGNHAAQFGALVDKFRADLTD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSSSSGAEATGVTTLLSYLASESEGSLKVQGWSASGGR
                     AEVVSDAEGTGGKAVKLTKEA"
BASE COUNT          327 a          476 c          355 g          359 t
ORIGIN      
        1 gcttccttgg tcagcttcac tgccttacct ccggttccct ccgcatcgct gaccacttct
       61 gccctgccgc cactggctga ccatccctgt actttcaggc ttccctctga ctcgctggca
      121 aggtaagaga gcagtgttgt gacgcctgtg gcttctgcac cggaagacga tgacgggcgc
      181 acctctgata ctgtcgatga tgcccccgcg tttagcgcca ctcttcccgc atggcgcaaa
      241 atcgccgttg ccagacggtc ggaaataatc ccgcggcgag cccatgaact gaaatggctc
      301 gccctgtcct gtgacgtcca ggtggctgag ctgtcacgcc atttcgaacc gtaatacccg
      361 atacccggaa tgtccgggtc ttcttccggt ttgttcgtcg gcacattcac cccgttctca
      421 tcggtcatga acggtacgaa atggatattc ttttccgttt tgtttttgta gctgccgtac
      481 accgtctggt acgaggattc gttcttctgc ttccagaaat acgtcgtatc tccacagatc
      541 cagggaacac cgccagcaga gccaccaacg cactgacctg ccatatccgt caggtctgca
      601 cggaatttat caaccagcgc accaaactgt gctgcgtgat ttccgggcgt gccgtcaaaa
      661 tcaaattccc cctgcatcca caccacggca aacagcacat ttttcgggtt cttcttcagt
      721 gctgcttttg ttcgaccgat aaggtcctta tacagcggct tgtccacacc ccagcgggtt
      781 gaattctccg aggcaccact cgcgtcactg tatgtgccat cagctccggt ggtgaacgct
      841 gaaccaccac gacagcacgg aaccagcaga atgcccgcat tcgccggtat aaacggcagc
      901 agttttttgg cgatatgcag cccctgcccc acggtaccgt actgcccttt tgacaggtcc
      961 gctttcggat ggttaagacg gctcatgtcc tgcacatcat gcagacaatg gtccgccgga
     1021 atgatgtcgt tatatttgca tgctgcaccg cccggtgtca ccgtactgcg gcgcgccagc
     1081 tgcttaatac gcgggtccgg acggtcatat gtctccggca gcggaagacc ttcaccatac
     1141 gacatgccat ttgactgccc tgccagaacc acaacaaagt aatactccgg gtcgctggtg
     1201 gcactgacag ccaccgcctt accctcatta ccggtcaccg ccactggtgt ggtgacatca
     1261 ccttccgccg caatggcctg catcagggta taaggcgtga tggccaccgg actgccaaat
     1321 ggctgccagc cctccttcag tttttgtgtc agtcgttcag caaggtctga cggcgatgcc
     1381 gccctgacca catcgtagtg tttaaatgtc ataacctctc ctgccgccag caaagtaatt
     1441 atccggaata cacaatcatg taacacataa aaaacacgct ataaaaaaac catgtctctt
     1501 atataacaaa aaaacgc
//
LOCUS       DAZFWL020000374         1509 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFWL020000374 DAZFWL020000000
VERSION     DAZFWL020000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.380"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..80
                     /locus_tag="ACHKNP_RS26910"
                     /old_locus_tag="pgaptmp_005382"
     CDS             <1..80
                     /locus_tag="ACHKNP_RS26910"
                     /old_locus_tag="pgaptmp_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707319.1"
                     /translation="GWFIGSIHDTEDGPVCVWLRNKAEA"
     gene            386..964
                     /locus_tag="ACHKNP_RS26915"
                     /old_locus_tag="pgaptmp_005383"
     CDS             386..964
                     /locus_tag="ACHKNP_RS26915"
                     /old_locus_tag="pgaptmp_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HGU4707320.1"
                     /translation="MRDIDVRKAVHAKILRDHHKDPDTLIIDEFTMNLGASRADIAVI
                     NGLIHGYELKSKSDNLLRLPAQVQHYSSVMDKVTLVVSDCHLYDALSIVPSWWGIKQV
                     TQGARQGIHLKTIRTSKLNPQVDKLSLTMLLWKDELLSLLSDVGELQNLKNKPKRVLW
                     SKLANSMDVGELREAVRVKLKARKEWRVAQQP"
     gene            complement(924..>1509)
                     /locus_tag="ACHKNP_RS26920"
                     /old_locus_tag="pgaptmp_005384"
     CDS             complement(924..>1509)
                     /locus_tag="ACHKNP_RS26920"
                     /old_locus_tag="pgaptmp_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HGU4707321.1"
                     /translation="EDKINSGELYTLAMGLINNLAHLNEYRKVILSGGSFPTDLSDIS
                     VGLYSQPRIEWILWQSLMNRKELARNVIYSDYGVQHPDFNRLSTRFPSVSASVRYSGD
                     NDFWVFRGRVANRFGYEQYGKHSEDILAHREYSGPTFCAGDRDIEYYANEYQAYKANP
                     SGNYKFGSPEVWRRIGQNHHITKVVEQLATLYGL"
BASE COUNT          467 a          298 c          315 g          429 t
ORIGIN      
        1 atggatggtt tattggttca atacatgaca ccgaagatgg cccggtttgt gtatggctga
       61 gaaataaggc cgaagcataa aggctataaa ccgactaaca actaaatact gaagatttaa
      121 atcagaaacg atttttatta aacccttaac cggagggatt ctgcaccctc agaacatcag
      181 gaggccgtct gaaagggcgg aacagataat gcttacgttg aaacatttta tcgacatacc
      241 aacatggtta gccgtcattg cttttgttaa aatacacatc cacttttctg tgcaatgttt
      301 aaccactggt catatcaaat ggcattcatg cgaaccatga tatagaatca tggcttgaga
      361 gagtcgatga aagcgcaact atggtatgag agacattgat gtaagaaagg ctgtgcatgc
      421 caagattctg agagatcatc ataaagatcc tgacacccta atcattgatg agtttacgat
      481 gaatctaggg gctagcagag ctgatatagc agtgatcaat gggcttatac atggttatga
      541 gttgaagagc aagagtgata acttgctcag attaccagcg caggtgcaac attactcatc
      601 agtgatggat aaagtaactt tggttgtctc tgattgccat ctttatgatg ctttaagcat
      661 agttccatca tggtggggga taaagcaagt tacgcaaggt gcacggcaag gtatccattt
      721 aaaaacaatt cgaactagca agttgaatcc acaagtggac aaactttcct taacaatgct
      781 tctttggaaa gatgaattgc tttccctatt aagtgatgta ggggagctac agaatttgaa
      841 aaataaacct aaacgcgtct tatggtcaaa actcgccaat agtatggatg ttggcgagct
      901 tcgtgaagct gttcgagtta aacttaaagc ccgtaaagag tggcgagttg ctcaacaacc
      961 ttagttatgt gatggttttg cccaatccta cgccatacct ctgggctacc aaatttatag
     1021 ttaccagagg gattggcttt gtaggcttga tactcgtttg cataatattc tatgtctcta
     1081 tctcccgcac agaatgtagg ccctgaatat tctcgatgag caagaatatc ctcactatgt
     1141 ttaccatatt gttcataacc aaagcgatta gctactcttc ctcgaaatac ccaaaagtca
     1201 ttatctccag agtatctgac gctggcagat acgctaggga atcgcgtcga aagcctatta
     1261 aagtcggggt gctgtactcc ataatcacta taaatcacat ttctggcaag ttcttttcta
     1321 ttcattaaac tctgccataa aatccactcg attcgaggtt gagaatatag accaacagaa
     1381 atatcactga gatctgtagg aaatgaaccc ccagaaagaa tcacttttct gtattcattg
     1441 aggtgcgcca gattgtttat caatcccatt gccaaagtat atagttcgcc ggaattaatt
     1501 ttatcctcg
//
LOCUS       DAZFWL020000375         1501 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFWL020000375 DAZFWL020000000
VERSION     DAZFWL020000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.381"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            150..350
                     /locus_tag="ACHKNP_RS26925"
                     /old_locus_tag="pgaptmp_005385"
     CDS             150..350
                     /locus_tag="ACHKNP_RS26925"
                     /old_locus_tag="pgaptmp_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4707322.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPYHQHA"
     gene            304..1041
                     /locus_tag="ACHKNP_RS26930"
                     /old_locus_tag="pgaptmp_005386"
     CDS             304..1041
                     /locus_tag="ACHKNP_RS26930"
                     /old_locus_tag="pgaptmp_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HGU4707323.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTRNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESTFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
     gene            complement(1172..1501)
                     /locus_tag="ACHKNP_RS26935"
                     /old_locus_tag="pgaptmp_005387"
                     /pseudo
     CDS             complement(1172..1501)
                     /locus_tag="ACHKNP_RS26935"
                     /old_locus_tag="pgaptmp_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          404 a          369 c          363 g          365 t
ORIGIN      
        1 ttttctttaa gtaaataatc acctccctga atcattgttg atgacttttc aatctatttg
       61 ttgtatcaat taaacaaatc gaatcgatcg tttttgtcga tcgattgcat gagaaaggca
      121 tcaacaacaa agcaggaggt tatgcggcca tgtatcgaaa catacggtgc cgacactgca
      181 ataagctact ggcacgggcc agtttcagtt atctggaagt aaaatgtccg cgctgtaaaa
      241 ccctcaatca aatcacatct ccgagcgcca cagagcaccc cacatacaca aggaaatcct
      301 gtcgtgggga acagaaagca agtcacatcc cgtatcatca gcacgcctga acttattcgc
      361 tataacgaca atatcgttgg ttatggctcc cgtgagttgc gggttgagac aataagttgc
      421 tggctggcca gactggtcat cgtcaataaa cattacagcc accgtttcgt aaataactcc
      481 taccttcatc tgggcatatt ttcagaacgg gagctggttg gcgtaatgca atggggctat
      541 gcccttaatc caaacagtgg tgcacgcgtc gtaacgggca cgcggaaccg tgaatatatg
      601 gagcttaacc ggttatggct gcatgactgt atgccgagaa actccgaatc aagggcaatc
      661 agttacgcgc tcaagctaat caggcagctt tatccgcagg tacagtgggt tcagtcgttt
      721 gcagatgagc gttgtggctg tcttggtgtc gtgtatcagg caagcaattt tgattatgta
      781 ggcagccacg aatcaacatt ctatgaactt gatggcgagt ggtatcacga gatttgcagg
      841 aatgccatta aacgaggcgg acagcgaggt gaacatctga gggctaatat cgacagagcc
      901 agtgttcata aattccgtca gtttcgttac atccggtttc tgaacaagag ggccagaaaa
      961 cgcctcaaca caaagctatt caaagtccag ccttacccaa aaccacaaac agttaaaact
     1021 ggtttaaaag agagtgagtg aacgcttaaa aactcattct aaaacccaaa agaaaagcgc
     1081 cgatttagta tcaaataagt tgcgtatcgg cgtttttcag cggtatcaaa tgaaatgcaa
     1141 atcggtatca aaaaaatcgc cgcgctatac acgtcatctc cggtttacgc gacagcgaca
     1201 tccaccccgt caggtcacgc tcccgccgcc atacccgttt cgccattcgc cgtcccagcg
     1261 ggtcgtagag gtagcggctc tcgaccagtg gctcgccatg ctgtatccgc gtgtagaaca
     1321 ccagacggtg ctggctgtcg tagtggtagt ggtgggtccg ctcgtcgtcc gtccggataa
     1381 cacccgccgg gataaggtcc gtcttctccg tcagtctgcc gtattcatca tagtggtaaa
     1441 catagtgcgc atcctctgcg atgcggttat ccggccacgc cgtgagggta ctgtccgggt
     1501 g
//
LOCUS       DAZFWL020000376         1470 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFWL020000376 DAZFWL020000000
VERSION     DAZFWL020000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.382"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..184)
                     /locus_tag="ACHKNP_RS26940"
                     /old_locus_tag="pgaptmp_005388"
     CDS             complement(<1..184)
                     /locus_tag="ACHKNP_RS26940"
                     /old_locus_tag="pgaptmp_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4707324.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDII"
     gene            complement(184..399)
                     /locus_tag="ACHKNP_RS26945"
                     /old_locus_tag="pgaptmp_005389"
     CDS             complement(184..399)
                     /locus_tag="ACHKNP_RS26945"
                     /old_locus_tag="pgaptmp_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4707325.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            542..940
                     /locus_tag="ACHKNP_RS26950"
                     /old_locus_tag="pgaptmp_005390"
     CDS             542..940
                     /locus_tag="ACHKNP_RS26950"
                     /old_locus_tag="pgaptmp_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707326.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            complement(1021..1179)
                     /locus_tag="ACHKNP_RS26955"
                     /old_locus_tag="pgaptmp_005391"
     CDS             complement(1021..1179)
                     /locus_tag="ACHKNP_RS26955"
                     /old_locus_tag="pgaptmp_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU4707327.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(1265..>1470)
                     /locus_tag="ACHKNP_RS26960"
                     /old_locus_tag="pgaptmp_005392"
     CDS             complement(1265..>1470)
                     /locus_tag="ACHKNP_RS26960"
                     /old_locus_tag="pgaptmp_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707328.1"
                     /translation="FLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLSTSFNAGS
                     KRFVIYQSAQDKNLQVDIVRTRI"
BASE COUNT          452 a          371 c          258 g          389 t
ORIGIN      
        1 gaatgatgtc ttttccggtg tgtccgtgac atacagtcca tacgccaacg atatcttcgt
       61 atggtatgta gctgacacct tccaggccat cgtcaccact cggaccagtg atgagcacag
      121 acgctatggc aacagcccca ccaccaatag cagcagcaac agccttgcgt aatgatggcg
      181 acattattca cctctcgcag ccttacgctt atcttcttta atcttgaaat aaaggtttgt
      241 cagatacgtc agcaagccaa acaccaggct acccaatacg cctattgcca cccactggga
      301 tggggagact ttgtccagca actgcagtag ccagtatccc gtccccaccg ctgacgtggt
      361 gtatgacaca cctgttgtta ttttttccat ctggtacata ccccgtctcc cgttatccgg
      421 aagctgacaa caataaaaag ccaccagtta attcctgatg gccctgatgc ataaacgtca
      481 taatacctga ctgttatgat tgacaataat gataatgttt atatagaaag gttcccgatg
      541 tgtgttacat atcatttctc cacggggaat atccccacgc cagcgcagac tcttttaccc
      601 gttctcttct gcgctggctc ttttttatta tgctgctgca tttacctctg gcaccatgct
      661 ttctatctca acacaatacg tggtacttct tgtaaccaat atcataacga ttaatcgaca
      721 tagaatttct cccgtgtaca ggaacagagt taaaaagccg gaaccggaat caaatcacag
      781 gatgaccatc tgccagtggc aggtcataaa aaaaaggccg cgccatgcgc agccagaact
      841 cacaaggaaa atgatagaag gaaataacat tagtgatgta cgcatggcgc ctcccgctaa
      901 gttctgcaat gatcaaacag aactcgctac gtgcccttaa aactcgatca tttagcccct
      961 ccaaggagga ttcaccatgc ggttgatttt ttaataaaca gtaaacaaaa aagtcaagaa
     1021 ttattcattc tgttctttca tcatcggcca cagcaatacc acaatgccgc agaccagagc
     1081 gccatcagtc agtaccaaca ttatcctgct ggtgaaatcc atcatcacca tcactaaaag
     1141 caggatcaca acagcaagca gacacagttt ataaaacaat gttcagaaaa cgcattcagc
     1201 atgcctaagg ttctattcct acgaatagcc aacttgcaac ttaaaatatt atttatgcag
     1261 ccaattaaat tctggtcctt acaatatcaa cctgaagatt cttatcttgt gctgattgat
     1321 aaatgacaaa ccttttacta cctgcattga aagaagtaga caaaaccaga caattatcat
     1381 aacgagcaag aacataatac caaccatcat tataattaat catttcatat tctttcttaa
     1441 actgtggttt gtaatatcct gtcagaaatg
//
LOCUS       DAZFWL020000377         1468 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFWL020000377 DAZFWL020000000
VERSION     DAZFWL020000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.383"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            9..356
                     /locus_tag="ACHKNP_RS26965"
                     /old_locus_tag="pgaptmp_005393"
     CDS             9..356
                     /locus_tag="ACHKNP_RS26965"
                     /old_locus_tag="pgaptmp_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HGU4707329.1"
                     /translation="MKVKKVQLLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNTDEV
                     QLQCLDGNPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSL
                     GDVTKAAFDLCDK"
     gene            362..1363
                     /locus_tag="ACHKNP_RS26970"
                     /old_locus_tag="pgaptmp_005394"
     CDS             362..1363
                     /locus_tag="ACHKNP_RS26970"
                     /old_locus_tag="pgaptmp_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4707330.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPQKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
BASE COUNT          420 a          241 c          367 g          440 t
ORIGIN      
        1 gtaatgatat gaaagtaaaa aaggttcaac tattagttac ttttttatct atgttttctt
       61 tttccgccgt cgcaatgcct tttaaaacta ttgaacgtga gagtttcaat ggggtatggc
      121 catttaatac tgatgaggtt caattacagt gtcttgatgg taatccttat gtgatgaatt
      181 ttgacgataa taagttatat gcgcttacag gtttagctcg aataaaaggt aaaacatttg
      241 gtgcgttacc gttagataac aataatccat tttggctaga taatgatgcc gccccagggt
      301 taaaaaagag tctgggggat gtcactaagg ctgcatttga tttatgtgat aagtaactaa
      361 aatgtcggtt cgtaagattc catcaggtaa atggctttgc gaatgttatc cctacggggc
      421 atcgggaaaa cgcattcgta aacagtttgc gacaaaaagt gaggcgctct cttatgagcg
      481 ccgtttaatg aatagtagag ttggagacga gtttcaagat ggttctggtc ctcgtctttc
      541 tgagttgatt gctcgttggt ttgagatgta cggtaaaacc ttgtcctctg gtgcagagcg
      601 caaagtcaaa cttgaggcga tttgttccag gctgggggat ccatttgctt ctcagtttga
      661 caaaaatatg tttgctactt atcgggaaag aaggctatca ggagaatgga atcccaaggg
      721 gaagaaaaaa cttagtgaag caaccgttaa tcgcgagcag tcatatctac atgctgtttt
      781 tgccgaactg aagcgccttg gggagtggtc tggtgaaaac cccctgactg gtattcgcaa
      841 gtttcgtgag gaagaaaagg aactggcgtt tctgtatgta gatgagattg aacgccttct
      901 gattgcgtgt gatgagtcac ggaataaaga tttgggggtt gttgtccgta ttgggcttgc
      961 gactggtgct cggtggagtg aagctgaagg attaaagcaa tctcaagtac tgcccggtcg
     1021 aatcacattt gttaaaacta aaggaaagaa gaaccgcact gtaccgattt cacctcaatt
     1081 gcaggctatg cttcctcaaa aacgaggagc gctattttca ccatgttatg aggcttttga
     1141 cgctgcaatt aagagagcga agatcgagct tcctgatggg caattaactc atgtgctacg
     1201 tcacacgttt gccagtcatt ttatgatgcg gggcggaaat attcttgtgt tgcaaaaaat
     1261 actggggcat agcgatataa aaatgactat gcgttatgcg cattttgctc caggtcattt
     1321 agaggctgct gttgaattga acccttttga caatagaggg taaaaagtgg cgataaaaag
     1381 tgacaataga tttaaatggc gttgtataga gccattttca aagttaataa aatcataagg
     1441 ttatgattta taaatggtgg ttgatagt
//
LOCUS       DAZFWL020000378         1466 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFWL020000378 DAZFWL020000000
VERSION     DAZFWL020000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.384"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..270)
                     /locus_tag="ACHKNP_RS26975"
                     /old_locus_tag="pgaptmp_005395"
     CDS             complement(<1..270)
                     /locus_tag="ACHKNP_RS26975"
                     /old_locus_tag="pgaptmp_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707331.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTS"
     gene            complement(365..571)
                     /locus_tag="ACHKNP_RS26980"
                     /old_locus_tag="pgaptmp_005396"
     CDS             complement(365..571)
                     /locus_tag="ACHKNP_RS26980"
                     /old_locus_tag="pgaptmp_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4707332.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     ERSSRWLKSEVEAWLQARIAQSRP"
     gene            1328..1453
                     /locus_tag="ACHKNP_RS26985"
                     /old_locus_tag="pgaptmp_005397"
                     /pseudo
     CDS             1328..1453
                     /locus_tag="ACHKNP_RS26985"
                     /old_locus_tag="pgaptmp_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
BASE COUNT          379 a          341 c          414 g          332 t
ORIGIN      
        1 actggtgcat aaattcacta tcagatcatc atccaggggc ggtgtggccc gtttgtcagg
       61 gccataaatc agccggtgca ggctctgggg tacgatggca atctccggtt tttcaccatt
      121 gaatgacacc caaccgggcg tcagattatg gaagtagtag atattcggat gacacacgcc
      181 ccaggatacg gcgtgccggg tgaagccggt cagccatttc tggtagcttt cgtggagcca
      241 ttcgtagcgg gtctgatact gttgtttcat tttcatattc ctcacgggga tataacaggc
      301 agcagaatgc cgccggcagg ccggagatta cgtctgctgc gggtgaacgg ataacggtca
      361 gaaattacgg acgggactgt gcaatacgcg cctgcagcca ggcttccact tcacttttca
      421 gccagcggga gctgcgctcc attttgatgg gggcaggaaa gcccccatct ttgatgagtt
      481 tgtaaaacca cttatcggtt aagccggtaa gttgagtgat aaacgccatg tcgaccatct
      541 ggtcatccat cagcgaaaca ggggtagcca tgagaatgtt cctccggatg ttgttaaacc
      601 agccggtgtg gtcatgaaaa cagcgcgcca ccttcactgg cataacaacc ggggagaaac
      661 gtaaaaaata atcgggaatg tggctgtccg gagataaacg gggggattgc gatggcagag
      721 ccgtagcgga atcccgggtg gttgttgcga aaagatgctt ccgcacgcac gcgcgataaa
      781 ggagggagga ctttcgtgag acaaggggaa aactagcgtt ctgataattg cagagttgtt
      841 ttcggaggtg gttacagtga gtcagcccgt tcctggcgag gaggagactg atatcaccga
      901 tgggataagg ctgtctgatt ggtattataa atataagaag tcatcagaga gtaacgtaaa
      961 ggatttgcca gagtaagcta agagagtaaa tctctccggt aacagagcgc tgtgaatcgt
     1021 tcatgtatca gagtgcagat acatgaacgc tgatgcgtaa aatcagcagt gacacgccag
     1081 ctccccctgt gcccattcct gtataagaca tgaactgagc gtattaatgc tcctgccctg
     1141 agactgagcc ggtgagtgag gggctgagaa atccctcttg acactgctct aaaccagaaa
     1201 tacttccccg gttgttgcgg tgcgtccgga tccccgataa cggaacacta aggctgtatt
     1261 gcgggacacc tagtaagtgg cagcccgtaa tacgtcagga gacgcgggaa aagtgaattc
     1321 ccacacgcgt tataaactta cccttgcgga gctggaagcc tttaactctg ccgttgacaa
     1381 ccggctggca gaactgacaa tgaacaaact ttacgatcgc gcgccggctt ccgtctggaa
     1441 atatgtcacc tgaatattga agcagc
//
LOCUS       DAZFWL020000379         1458 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFWL020000379 DAZFWL020000000
VERSION     DAZFWL020000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.385"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(12..>1458)
                     /locus_tag="ACHKNP_RS26990"
                     /old_locus_tag="pgaptmp_005398"
     CDS             complement(12..>1458)
                     /locus_tag="ACHKNP_RS26990"
                     /old_locus_tag="pgaptmp_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HGU4707333.1"
                     /translation="SSLRALVAKLQRMLFGRSSEKSREKIEKKIARAETRITELQNRL
                     GEAQLQLTSMAGETAPKTSDSPVRKALPATLPRDRQVISPAETECPVCSGKLKPLGES
                     ISEQLDIINTAFRVIETVRPKLACSRCDCIVQAPQPPKPIERSYASPALLARIIMAKF
                     AEHLPLYRQSEIYARQGVELHRNTMGRWVDIMGEQLRPLYDELKHYVLMAGKVHADDT
                     PVNVLEPGQGKTRTGRLWVYVRDDRNAGSTMPAAVWFSYSPDRKGIHPQQHLADYRGI
                     LQADAYAGYNALYESGQVTEAACMAHARRKIHDVHVRHPTTVTGEALRRIGELYAIEA
                     EIRGSPAEERLAVRKARTVPLMQSLYEWLQGQMSTLSRHSDTAKAFTYLLKQWDALNE
                     YCRNGWVEIDNNLCENALRVVALGRRNYMFFGSDGGGESAAVMYSLIGSCKLNGIEPE
                     TWLRHVISVINTWPANRVKELLPWNVTQSVN"
BASE COUNT          271 a          421 c          403 g          363 t
ORIGIN      
        1 tggggtaaaa attagtttac agattgagtg acattccagg gcaacaactc tttcacgcgg
       61 ttggcaggcc aggtgttgat tacactgatc acgtggcgta gccacgtttc cggctcgatt
      121 ccgttaagtt tgcagctacc gatcaggctg tacatcactg ccgcactctc gcctccacca
      181 tcagagccga agaacatgta gttacgccgc cccagtgcaa ctacccggag ggcgttttca
      241 cacaggttat tgtcgatctc cacccagcca ttgcggcagt attcgttcag agcgtcccat
      301 tgcttcagca gataggtgaa cgctttcgct gtatccgagt ggcgcgacag cgtgctcatc
      361 tgcccctgga gccactcata caacgactgc attagcggta ccgttctggc ttttctgacc
      421 gccagtcgct cttctgccgg actgccgcgg atctcagcct cgatagcgta cagttccccg
      481 atacgacgga gcgcttctcc cgttactgtc gttggatggc ggacatgtac atcgtggatc
      541 ttgcgtcggg catgtgccat acaagccgct tcggttacct gaccgctttc gtaaagagca
      601 ttgtaacccg catatgcatc ggcctgcagg atacctctgt agtccgccag atgttgctgt
      661 gggtggatgc ctttgcggtc gggagagtat gagaaccaca ccgctgccgg catggtcgaa
      721 ccggcgttgc gatcgtcacg aacatagacc cacagccgtc cggtacgggt tttaccctga
      781 cccggctcca gtacatttac cggcgtgtca tcggcatgca ctttacccgc catcagcaca
      841 tagtgcttca gttcatcata cagcgggcga agctgctctc ccatgatgtc aacccagcgc
      901 cccatcgtat tgcggtgcag ctccacgccc tggcgggcat agatttccga ctgacggtac
      961 agcggcagat gctcggcgaa cttagccatg attatgcggg ccagcagagc cggactggcg
     1021 taactgcgct cgatgggttt tggtggctgc ggagcctgaa ctatacagtc gcaccggctg
     1081 caggccagtt ttgggcgaac cgtttcgatt accctgaacg cggtgttgat gatatccagt
     1141 tgttcagaga tgctttctcc cagcggtttc agtttgccgc tgcagacggg gcattcggtt
     1201 tctgccgggg agataacctg cctgtcacgg ggaagtgttg ccggaagtgc tttgcggacg
     1261 ggagagtctg atgttttcgg cgctgtctct ccggccattg aggtgagttg caactgcgcc
     1321 tcaccaagcc tgttctggag ctcggttata cgcgtttctg cccgtgcgat cttcttttct
     1381 atcttctcgc ggcttttctc gctgctgcga ccgaacaaca ttctctgcag tttagcgacc
     1441 agcgctctga gtgagctg
//
LOCUS       DAZFWL020000380         1453 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFWL020000380 DAZFWL020000000
VERSION     DAZFWL020000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.386"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..583
                     /locus_tag="ACHKNP_RS26995"
                     /old_locus_tag="pgaptmp_005399"
     CDS             <1..583
                     /locus_tag="ACHKNP_RS26995"
                     /old_locus_tag="pgaptmp_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4707334.1"
                     /translation="PSTVDPSNFDGSTVDKKLPIRGPMIDPDPSVLKPDPSDKRSSCP
                     DASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRIVSLYEQAASYD
                     GEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVKNIMSPAKLREK
                     WDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            624..1046
                     /locus_tag="ACHKNP_RS27000"
                     /old_locus_tag="pgaptmp_005400"
     CDS             624..1046
                     /locus_tag="ACHKNP_RS27000"
                     /old_locus_tag="pgaptmp_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4707335.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLETKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKPKKPSAVDPDLIWSLPDG
                     EIRRYGRRLNIICLECRKSEVMQRVLAFYQGNFEEVVR"
     gene            1043..1297
                     /locus_tag="ACHKNP_RS27005"
                     /old_locus_tag="pgaptmp_005401"
     CDS             1043..1297
                     /locus_tag="ACHKNP_RS27005"
                     /old_locus_tag="pgaptmp_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707336.1"
                     /translation="MSESKCQVNGNKIEPCAALAKSLEHDAEYTMRKGLLIYKIWNES
                     LTRGPDFVMLRSGEFSKLPVRVSFCPFCGESLKTWENRNE"
     gene            1290..>1453
                     /locus_tag="ACHKNP_RS27010"
                     /old_locus_tag="pgaptmp_005402"
     CDS             1290..>1453
                     /locus_tag="ACHKNP_RS27010"
                     /old_locus_tag="pgaptmp_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707337.1"
                     /translation="MNEIKEIPVVRDEYGCWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRD"
BASE COUNT          407 a          296 c          407 g          343 t
ORIGIN      
        1 cccctcaacg gttgacccgt caaattttga tggatcaact gttgataaaa aactgccgat
       61 tagggggccg atgattgacc ccgatccgtc agtattaaaa cctgatccgt cagataaaag
      121 atcttcttgt ccggacgctt cgcaaccgga cccgcagacg gctgaacagg attttttaac
      181 ccgacaccct gacgcggttg tgttcagtgc gaaaaaacgc cagtggggaa gtcaggaaga
      241 tttggtgtgc gcacagtgga tctggggacg aatcgtgagt ctttacgagc aggcggccag
      301 ctatgatggc gagatcacta gaccgaaaga acccaactgg acagcatggg ccaatgacgt
      361 tcgcacaatg cggatgctgg atggcagaac tcacagacaa atttgtgaaa tgtttgggcg
      421 tctccagcgg gattcgttct gggtaaaaaa catcatgagt ccggcaaaac tccgggaaaa
      481 atgggatgaa ctggttatcc gcctggggcg ttcgcctgcg cagcgttgcg tgaatcacat
      541 ttctgaaccg gacactgaaa ttccgccggg cttcaggggg taagtgttaa tttctggtca
      601 tgaggtaatt ttcaggaggg cttgtggcaa aagtttttac acaagaagag cgggaaaaaa
      661 ttaaagggca ggttgttgaa ctcgtacgcc agagtgggcg cgagacgtta cgacaactgg
      721 aaactaaaac tggggcaaca agatatctga tgagcgttct ggccagagag ctggttgcca
      781 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      841 ggcaaaatgc ccgtaaaaag ctctcaaggg caaagccgaa gaaaccatct gcggttgatc
      901 cggaccttat ctggtcatta ccagatggcg aaatacgccg ctacggcagg cgtctgaaca
      961 taatctgtct cgagtgccgg aagagcgaag ttatgcagcg cgtactggcg ttttatcagg
     1021 ggaattttga ggaggtggtg cggtgagtga atcaaaatgc caggttaatg gcaacaagat
     1081 agaaccatgt gcagcactgg caaagtccct tgagcatgat gctgaataca cgatgcgaaa
     1141 aggtctgctg atatacaaaa tctggaatga gagtttaact cgcggtcctg attttgtgat
     1201 gttgcgttcc ggtgaatttt ctaaattacc agttcgggtt tcattttgtc cgttctgtgg
     1261 tgaaagtctg aaaacgtggg agaacagaaa tgaatgaaat taaagaaata ccagtagtac
     1321 gtgatgaata tggctgctgg acgcatcctg aatatgaaaa attctgtgac ggtcgggaat
     1381 atatttcaac ggaagagttt aacgcctgga tggaggaaaa taatcttcaa tacgtcctct
     1441 gcttcagaga tga
//
LOCUS       DAZFWL020000381         1450 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFWL020000381 DAZFWL020000000
VERSION     DAZFWL020000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.387"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..223)
                     /locus_tag="ACHKNP_RS27015"
                     /old_locus_tag="pgaptmp_005403"
     CDS             complement(<1..223)
                     /locus_tag="ACHKNP_RS27015"
                     /old_locus_tag="pgaptmp_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4707338.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLKR
                     GNDGQPPWFEHDQMTVAAKILLRCTWLDPA"
     gene            409..1377
                     /locus_tag="ACHKNP_RS27020"
                     /old_locus_tag="pgaptmp_005404"
     CDS             409..1377
                     /locus_tag="ACHKNP_RS27020"
                     /old_locus_tag="pgaptmp_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HGU4707339.1"
                     /translation="MSQPGSLCVDIDVSKAILDIAASSAIEQFSVGNDSDGFDAIIAE
                     LRMYAIALVLMKATGGLETAIVCALQAEGFELAVVNPRQARDFARAMGYLAKTDSIDA
                     RVLSQMAEVLNRHPERERFIRALPDTECQVLTAMVVRRRQLITMLVAERNRLHPAHPL
                     NRKSINIIIKALEDELARLDADMNSHIHSHFKELAKRLSSIKGVGTMTAAALLAEVPE
                     QGNLSRREISVLVGMVPVNRDSGTMRGRRTIFGGRAGVRTALYMAALVATRFNPVIKV
                     FYMRLLAAGKPKKVALVTCIRKLLTILNAMLRKNEEWNESYHHVAP"
BASE COUNT          363 a          356 c          380 g          351 t
ORIGIN      
        1 ctgccgggtc aagccaggtg catcgcagca ggattttcgc agcaacagtc atctggtcat
       61 gttcaaacca cggcggttgt ccgtcatttc cccgcttaag tgttaacgga caaccacagg
      121 acacacagca caaaacgtcc tgtcggttgt gcttcgcggc ttctttcgcc gtaacaaaat
      181 ggcctttgct gtcattcgcc atatagcagt tcagaattcg catatctcct ccttgtatct
      241 tgacaattgg ttaaaataac tgtgattaga tttggcaagg tgtatgcagc ccgatccctc
      301 aaggggttca tagcccgtag ctcagcgaat ggcgcataca ctgagccact catctgaaac
      361 ccgaacagtt gtcagtacgc cataacgcat ttgcaaggat gggtaatcat gagtcagcca
      421 ggttcacttt gtgtggatat cgatgtttcg aaagcgatac tggatattgc cgccagcagc
      481 gctattgaac aattttctgt cggcaatgac agtgatggct ttgacgccat cattgctgag
      541 ttgagaatgt acgccattgc gctggtttta atgaaagcca ctggaggact tgaaaccgcc
      601 atagtctgtg cgcttcaggc agaaggtttt gagcttgccg tggttaaccc cagacaagct
      661 cgtgactttg cccgcgccat gggatacctc gcgaaaactg atagtattga tgccagagtg
      721 ctttcccaga tggctgaggt tctcaaccgg catccggagc gggaacgctt tatcagagca
      781 ctgccggata ctgaatgtca ggttctcact gcaatggtag ttcgtaggcg gcagttgatt
      841 acgatgctgg ttgccgagag aaaccggttg caccctgctc atccattgaa caggaaaagc
      901 attaatatca tcatcaaagc actggaagac gagcttgcgc gactagatgc tgacatgaac
      961 agccatatcc atagccactt caaagagctt gctaagcgac tgagcagtat taaaggtgtt
     1021 ggcaccatga cggctgctgc gctgctggcg gaagtacccg aacagggtaa cctctccagg
     1081 cgtgaaatca gtgtgcttgt cggtatggtt ccagtaaacc gggactcggg cacgatgaga
     1141 gggcgacgaa ccatctttgg cggcagggct ggtgtgcgaa cagccctgta catggcagcg
     1201 cttgtcgcaa cccgctttaa cccggtaata aaggtgtttt acatgcgact gcttgccgca
     1261 ggaaaaccca aaaaagtggc gctggttacc tgtatacgca agctgctgac tatcctgaac
     1321 gcaatgctca gaaagaacga agaatggaat gaatcgtacc atcacgttgc tccataattt
     1381 ttacgttcaa gacagttgct gagctgtccg gggaagactg aaacctccgg tctggcagtg
     1441 tgtgcagtac
//
LOCUS       DAZFWL020000382         1450 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFWL020000382 DAZFWL020000000
VERSION     DAZFWL020000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.388"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..214
                     /locus_tag="ACHKNP_RS27025"
                     /old_locus_tag="pgaptmp_005405"
     CDS             <1..214
                     /locus_tag="ACHKNP_RS27025"
                     /old_locus_tag="pgaptmp_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707340.1"
                     /translation="NAALGTGTTSSAGEHAHNVYIGAHNHTIGIGAHAHSVIIGPHGH
                     TITVNATGNEENTVKNIAFNYIVRLA"
     gene            214..792
                     /locus_tag="ACHKNP_RS27030"
                     /old_locus_tag="pgaptmp_005406"
     CDS             214..792
                     /locus_tag="ACHKNP_RS27030"
                     /old_locus_tag="pgaptmp_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4707341.1"
                     /translation="MTFRMSEHSRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDSLFISELGPLPENVTWLSP
                     YGEYQKWNGTSWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLDIATEEE
                     ASLLAAWKTYRVLLNRVNTAVAADVEWPVAPQ"
     gene            complement(836..1408)
                     /locus_tag="ACHKNP_RS27035"
                     /old_locus_tag="pgaptmp_005407"
     CDS             complement(836..1408)
                     /locus_tag="ACHKNP_RS27035"
                     /old_locus_tag="pgaptmp_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HGU4707342.1"
                     /translation="MLCYLIAIHFVIFGNSKALLSYWMAEIIRREKMDVWRLLREKKQ
                     RNRNFLFWWRLANEMYINGNKLHKKAARKLNNNIINRFGCEIGLGANIGKGLTIPHHT
                     GIVVHFAVDAGENLVLRQNTTIGQVDGDMPGSRVKIGNNVDIGANCCIIGLSRKIGDN
                     VKIGAMSFINKDIPSNCTYITKKSGVVLYK"
BASE COUNT          428 a          313 c          291 g          418 t
ORIGIN      
        1 taacgctgcg ttaggtactg gtaccacgtc gagcgcaggc gagcatgctc acaatgtata
       61 tatcggtgcc cataaccaca ctatcggcat tggtgctcat gcccattctg tcattattgg
      121 tccccacgga cacaccatca ccgttaacgc tacgggtaac gaagaaaaca ccgtaaaaaa
      181 catcgcattt aactatattg tgaggcttgc ataatgacat tcagaatgag tgaacactca
      241 cggaccataa aaatttataa tctactgacc ggaaccaatg agtttattgg tgaaggtgat
      301 gcatacattc cacctcatac aggtctgcct gcaaacagta ccgatattgc cccgccagat
      361 attccggctg gcttcgtggc cgttttcaac agtgatgagg catcgtggca tctcgttgaa
      421 gaccatcggg gtaaaacggt ttatgacgtg gcatcagggg actcgttatt tatttctgaa
      481 ctcggtccat taccggaaaa tgttacctgg ttgtcgccgt atggagagta tcagaagtgg
      541 aacggcacat cctgggtgaa agatgcagaa gcagaaaaac tgtttcggat aagggaggcg
      601 gaagaaacaa aaaacagcct gatgcaggta gccagcgagc atattgcgcc acttcaggat
      661 gccgtagatt tggatattgc aacggaggaa gaggcatcgt tactggctgc atggaagaca
      721 taccgggtat tgttgaatcg tgttaataca gcggtagcag cggatgttga gtggccagtc
      781 gccccacaat aaagagaaaa agccatcgat tgaaatatag atggctttat gtactctatt
      841 tatacaatac aacaccgctc tttttagtta tatatgtgca gttcgatggt atatctttat
      901 ttataaaaga cattgcacct atttttacat tatccccaat tttacgtgat aatccaatga
      961 tgcaacaatt agctccgata tcaacgttat taccaatttt tactcttgaa ccaggcatgt
     1021 caccatctac ctgtccaatg gtagtattct gtcgcaaaac cagattttca ccagcatcaa
     1081 cagcaaaatg aacaacaatt ccagtatgat ggggaattgt taaacctttt ccaatatttg
     1141 cgcccaatcc aatttcgcaa ccaaatctgt taattatatt attgtttaac tttctggctg
     1201 ctttcttatg taatttattg ccattaatat acatttcatt agccagtctc caccagaaaa
     1261 ggaaattccg attgcgctgc ttcttctctc ttaatagtcg ccaaacatcc attttttcac
     1321 gcctgatgat ttcagccatc caatagctca atagagcttt tgaattcccg aagatgacaa
     1381 agtgaatagc tattaagtaa cataacatat tgcaactgcc tatatttttt ggtttctatg
     1441 gttatatatt
//
LOCUS       DAZFWL020000383         1440 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFWL020000383 DAZFWL020000000
VERSION     DAZFWL020000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.389"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            35..1360
                     /gene="nleA"
                     /locus_tag="ACHKNP_RS27040"
                     /old_locus_tag="pgaptmp_005408"
     CDS             35..1360
                     /gene="nleA"
                     /locus_tag="ACHKNP_RS27040"
                     /old_locus_tag="pgaptmp_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HGU4707343.1"
                     /translation="MNIQPIVTSGITTQNNRHHHAEQTSPTQIPQSELPNGCETGFVV
                     HIPEDMQRHAPECGETTALLSLIKDEGLLSGLDKYLAPHLEEGSAGKKALDMFGLFNV
                     TQMALEIPSSVSGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGFNFKDLPGPL
                     KNALSNSNISTTAVSTIASTGTSATTSTVTTASKDPIPWFGLTAQVVRNHGVELPIVK
                     TENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDL
                     DNPNTKYAVLTNAAANALGAVGGFAVSRFTGTDPMLSPHIGAMVGQAAGHAIQYNTPG
                     LKPDTILWWAGTTLGLADLNKAETGEGRFTDYPRIWWHAREGAIFPNKEDIARATGAD
                     IRAMEEGIPVGQRHPNPEDVVIDIESNGSPHHNPSNYVDTFDIIQETRV"
BASE COUNT          461 a          299 c          302 g          378 t
ORIGIN      
        1 attaatggat ataaacatgc aataaggatt tatcatgaac attcaaccga tcgtaacatc
       61 cggaatcacc acacaaaaca atcgacatca tcacgcagaa caaacgtccc ctacacaaat
      121 accgcaatcc gaattaccta atggatgcga aacgggattt gttgttcata tcccagagga
      181 tatgcagcga catgcaccgg aatgcggtga aacaacagct ctactgagct tgataaaaga
      241 tgaaggtctg ctctctgggc tggataaata tcttgcacct catcttgaag aaggctctgc
      301 aggaaaaaaa gcattggata tgtttggttt attcaatgtt acccaaatgg cattagagat
      361 acctagttcc gtttcaggca tctctggtaa atatggtgtc cagctaaaca ttgtaaaacc
      421 agatattcat cctacatcag gtaattattt tttacagata ttccctctgc atgatgaaat
      481 aggttttaat tttaaagacc ttcctggccc gttaaaaaat gcattaagca acagtaatat
      541 atcaaccact gcagtgtcga ctattgcatc gactggaaca tcagccacta cttcgacggt
      601 aaccaccgcg tcaaaagacc caataccatg gtttggatta acagctcaag tagttcgtaa
      661 tcatggtgtg gaacttccta tagtcaaaac tgaaaatgga tggaagcttg ttggagaaac
      721 tcctcttact cctgatggcc ccaaagcaaa ttatactgaa gagtgggtga tcagaccggg
      781 agaagcagat tttaaatatg gtgcatctcc actacaggca actctagggc tggagtttgg
      841 cgcacatttc aagtgggatt tagataaccc taatactaaa tatgccgttc ttaccaatgc
      901 tgccgcaaat gcgcttggtg ctgtaggggg atttgcagta tccagattta ctggtacaga
      961 tccaatgtta agtcctcata tcggtgcaat ggttgggcaa gcagcggggc atgccataca
     1021 gtataatacc cccggattaa agccagacac tattttatgg tgggcaggta cgactctggg
     1081 gctggcagat ttaaacaaag ccgagacagg tgaaggcaga ttcactgact atcctcgtat
     1141 atggtggcac gcaagagaag gtgccatctt ccctaataaa gaagatattg cccgtgcaac
     1201 aggggctgat atacgagcta tggaagaagg tatacccgtt ggacagcgac atccaaatcc
     1261 agaggatgtg gtcatcgata tcgaaagcaa tggctcacca catcataacc catcaaatta
     1321 tgttgatacc tttgatataa tccaagaaac aagagtctaa gctttactta tttatttaaa
     1381 aataatagct gaaatggatg acagctatta ttaataagga ttaatatatc tttttcctaa
//
LOCUS       DAZFWL020000384         1432 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFWL020000384 DAZFWL020000000
VERSION     DAZFWL020000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.390"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..170)
                     /locus_tag="ACHKNP_RS27045"
                     /old_locus_tag="pgaptmp_005409"
     CDS             complement(<1..170)
                     /locus_tag="ACHKNP_RS27045"
                     /old_locus_tag="pgaptmp_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4707344.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSP"
     gene            complement(226..558)
                     /locus_tag="ACHKNP_RS27050"
                     /old_locus_tag="pgaptmp_005410"
     CDS             complement(226..558)
                     /locus_tag="ACHKNP_RS27050"
                     /old_locus_tag="pgaptmp_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU4707345.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(568..>1432)
                     /locus_tag="ACHKNP_RS27055"
                     /old_locus_tag="pgaptmp_005411"
     CDS             complement(568..>1432)
                     /locus_tag="ACHKNP_RS27055"
                     /old_locus_tag="pgaptmp_005411"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4707346.1"
                     /translation="IKPVWALANDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHS
                     NYGAALEKQGVEITLIYSGSHKVDGNPYSHLPDDVRETLQYRMDATRQMFAQKVSAYT
                     GLSVQAVLDTEAAVYSGQEAIDAGLADELVNSTDAITVMRDALDARKSRLSGGRMTKE
                     TQSTTVSATASQADVTDVVPATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEA
                     HGREEQARVLAETPGMTVETARRILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPAS
                     DAVNDLLNTPV"
BASE COUNT          282 a          419 c          411 g          320 t
ORIGIN      
        1 atcggcgaaa cgtacagcgc catgtttacc agtcccggaa tttgtgagag atagactttc
       61 tccgtggtga agggatagct ctcacggaaa aagagacgca gaaacagcgg atcaaactta
      121 aatttctgct catttgccgc cagcagctgg gcggttgtgt acatcgacat aaaaaaatcc
      181 cgtaaaaaaa gccgcacagg cggcctttag tgatgaaggg taaggttaaa cgatgctgat
      241 tgccgttccg gcaaacgcgg tccgtttttt cgtctcatcg ctggcagcct ccggccagag
      301 cacatcctca taacggaacg tgccggactt gtagaacgtc agcgtggtgc tggtctggtc
      361 agcagcaacc gccagaatgc caacggcagc accgtcggtg gtgccatccc acgcaaccag
      421 cttacgggtg gcggtgtcca gcatcagcgg ggtcattgca ggcgctttcg cactcaatcc
      481 gccgggcgcg gttgcggtat gagccgggtc actgttgccc agcggctggt aatgggtaaa
      541 ggtttctttg ctcgtcataa acatccctta cactggtgtg ttcagcaaat cgttaacggc
      601 atcagatgcc gggttacctg cagccagcgg tgccggggca ccctgcatca gacgatccag
      661 cgcagtgtca ctgcgcgcct gtgcactctg tggtgctgcg gccagaatgc ggcgggccgt
      721 ttccacggtc ataccggggg tttctgccag cacgcgtgcc tgttcttcgc gtccgtgagc
      781 ctcctcacag ttgaggatcc ccataatgcg gctgttttct gccgcaaccg ctgcggtgat
      841 ctgcgcgttc acgtccggct gcgccgcgct ggcgttttcg ccctccgtcg ctggcaccac
      901 gtcagtaacg tcagcctgcg aagcagtggc tgaaacagtt gttgattgag tctctttggt
      961 cattcgccct cctgagagac gggatttacg tgcatccagt gcatcacgca tgacggtgat
     1021 cgcatcggtg ctgttaacaa gttcatcagc cagtccggca tcaatggcct cctgaccgct
     1081 gtacactgca gcctcggtat ccagcacagc ctgcacggac aggccggtat atgccgacac
     1141 cttctgcgca aacatctggc gggttgcgtc catccggtac tgcagtgtct cccggacgtc
     1201 atccggaaga tggctgtagg ggttgccatc caccttatgg ctgccgctgt aaatcagcgt
     1261 gatttccaca ccctgtttct ccagcgcagc accgtaatta ctgtgagcca tcatgacgcc
     1321 gatggagcct gtccgggcgg tctgcgtgac cagacgccgg gaggcggcac tggcaagcaa
     1381 ctgacctgca ctgcagttca tgtcgttggc aagcgcccat accggtttta tg
//
LOCUS       DAZFWL020000385         1430 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFWL020000385 DAZFWL020000000
VERSION     DAZFWL020000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.391"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            37..411
                     /locus_tag="ACHKNP_RS27060"
                     /old_locus_tag="pgaptmp_005412"
     CDS             37..411
                     /locus_tag="ACHKNP_RS27060"
                     /old_locus_tag="pgaptmp_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4707347.1"
                     /translation="MRIEFVLLYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGLVKK"
     gene            408..1229
                     /locus_tag="ACHKNP_RS27065"
                     /old_locus_tag="pgaptmp_005413"
     CDS             408..1229
                     /locus_tag="ACHKNP_RS27065"
                     /old_locus_tag="pgaptmp_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4707348.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
BASE COUNT          371 a          285 c          412 g          362 t
ORIGIN      
        1 tttgcaaccg gcgtgctcgg gtaaaagagg ttactgatgc gtatagagtt tgttttgctt
       61 tacccgccga cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      121 gccggtgagc gttatcgccg ggctgtggcg cttattgttc gccagcagcg gctgaaatta
      181 agcctgtccg gaaggctggc gataaagatt attgccgagc caccggataa gcgccgccgt
      241 gacctggaca atattctgaa agcgccgctg gatgcgctga cgcatgcggg agtgttaatg
      301 gacgatgagc agtttgatga aatcaatatc gttcgtggtc agccagtatc tggtggacgt
      361 ctgggggtga agatttaccc cataatgctt gaagggctgg tcaaaaaatg aaactggaag
      421 atttaccgaa atactactcc ccaaaatccc ccggcctgac tgatgcatcg gcctcaacgt
      481 cgaaagatac gctgagtatc actgatgtga tggccgcgca gggcatgaca cagaattggg
      541 ctgagatggg gttttctgcg ttccttggga aaatgggcat tagtatgaat gacagagagc
      601 gggcaacaga attgctgaca gaatatgcac tcagtcggtg tgatcgcgtg gcggcgttaa
      661 gaaaactccc ggcagaaata aaaccggcag tgatgcgtat tatggcttcg tatgcgtttg
      721 aagattatgc ccgtagcgcg gcgagcaaaa aacagtgccc ctgttgtcac ggaaaaaaat
      781 ttattgaaag cgaggttttt acaaacaaga tccagtatcc ggatggtaag ccgccagtgt
      841 gggcaaagtg cacaaaaggc gtgtatccgt cttactggga ggaatggaaa aaagtcaggg
      901 aggtggtaaa agttgcctgt ccggagtgtg gagggaaggg ggaggtttcc accgcctgta
      961 aagattgtcg tgggcgcggt gttgccattc atcgtgaaga gtcggtaaaa cgtggtatgc
     1021 ctgttatcag agactgccag cgttgtggtg gtcgtggcta tgaaagatta ccttcaacgg
     1081 aggcatttaa tgccatatgt aatgtaaccg atgccatatc tcttgataca tggaaaaaaa
     1141 cagttaaacg tttttacgat acgctggtgg tgcagtttga tattgaagaa gcatgggcag
     1201 aacaacaact gaaaaaggtg accagatagc tttgttgatt tttcccgaat ctgtggtaaa
     1261 tttgccctaa cgatgggcgt tttatgcctg acgttagaag attttttaca cccgtcgcca
     1321 ggcgggtttt tttatgactg aaatcacgcc agtacagtaa acgcgctggt ggttgtgaat
     1381 accggtcttt cagcttgctg gctttttcga caagagttat tggtgtgtca
//
LOCUS       DAZFWL020000386         1426 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFWL020000386 DAZFWL020000000
VERSION     DAZFWL020000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.392"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..414
                     /gene="yqeJ"
                     /locus_tag="ACHKNP_RS27070"
                     /old_locus_tag="pgaptmp_005414"
     CDS             <1..414
                     /gene="yqeJ"
                     /locus_tag="ACHKNP_RS27070"
                     /old_locus_tag="pgaptmp_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HGU4707349.1"
                     /translation="YSYTAEKMIYNETCTANWVIFNDQGRANLTIDFMYNKKNKTGTV
                     ALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTSKKVNKFEIMDQVDDDRLAQLIPD
                     FYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR"
     gene            complement(447..527)
                     /locus_tag="ACHKNP_RS27075"
                     /old_locus_tag="pgaptmp_005415"
     CDS             complement(447..527)
                     /locus_tag="ACHKNP_RS27075"
                     /old_locus_tag="pgaptmp_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707350.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(563..988)
                     /gene="yqeK"
                     /locus_tag="ACHKNP_RS27080"
                     /old_locus_tag="pgaptmp_005416"
     CDS             complement(563..988)
                     /gene="yqeK"
                     /locus_tag="ACHKNP_RS27080"
                     /old_locus_tag="pgaptmp_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HGU4707351.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKSSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            1182..>1426
                     /locus_tag="ACHKNP_RS27085"
                     /old_locus_tag="pgaptmp_005417"
     CDS             1182..>1426
                     /locus_tag="ACHKNP_RS27085"
                     /old_locus_tag="pgaptmp_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707352.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQAILSAMSI"
BASE COUNT          530 a          238 c          227 g          431 t
ORIGIN      
        1 tattcttaca ctgcagaaaa aatgatatat aacgaaacat gcactgcaaa ttgggtaatt
       61 tttaatgacc agggacgagc aaatcttacc atcgacttta tgtataacaa aaaaaataaa
      121 acaggaactg ttgcgttgag tggaacctgg caacaaggta acagagaaag taaatcaata
      181 cggcgaaata ttgaatacac atggattgaa aactatgaca cagcccattt aacatcaaag
      241 aaagttaata agtttgagat tatggatcaa gttgatgatg atagacttgc acaacttatt
      301 cctgattttt atgtctttcc agaaaaaagt gtaagctata atatactaaa gcaaggtaag
      361 catgctttta ttttgagcat tggtaacaga gcaataatgc attgtgcaag gtaaactaaa
      421 atagataaca aaaagatgga acctcgttaa tcataaaaca ataaaattaa atattctcgc
      481 agtatatggc agtctaaagc atcaaagttt tgatcaacat ctttcatttt agacatctcc
      541 ttgcaatgta aaatatcatg tatcaacaac atctggtgca aatccatgag tcgaactcga
      601 cattcatctt agctcgatta ttattccttc gtacagtcga tgtaaacaat acagaaagag
      661 gattattaag aaccagttta ctcagtgaat caatagagga aaggactaac gtttctttaa
      721 aagaattgat ttcatcatct gttaaactaa actcatcatt gacagatcgt gagatataac
      781 tgcttttaac tttactcttt acgttgactt tattgacaga attaacattc acaaatcttg
      841 aatttaatgc ccatgatgtt gtttgcgtga gaaccttctc agcattaagg aattttttta
      901 acggaatcct tggtttcttt ttcgagtccg aatttacaat atcatgcata tagaccattt
      961 catgaatctg cgaaaactca atgtccatta tatacctcac atttttaccg tgactcgatg
     1021 ttactgttca ataatcacct tccatcaata ctaaaattaa tacccctaat gtgccgataa
     1081 caaatatagt cattctacgt aatgtctcca taaggtgata tttgacatta tcagaagctg
     1141 cgaattcgga ttttgctcta atcaaatgag agaaatgtaa aatgaagcca cgaaatatta
     1201 ataatagcct accactgcaa ccattagttc ctgatcagga gaacaaaaat aagaaaaatg
     1261 aagagaaatc cgttaatcca gttaaaatca caatggggtc tggtttaaat tatattgaac
     1321 aagaatctct tggaggaaaa tatctaacac atgatttgtc aataaagata gcggatattt
     1381 ctgaagagat aattcagcaa gcaatattat ctgctatgag catata
//
LOCUS       DAZFWL020000387         1417 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFWL020000387 DAZFWL020000000
VERSION     DAZFWL020000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.393"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..292
                     /gene="tnpB"
                     /locus_tag="ACHKNP_RS27090"
                     /old_locus_tag="pgaptmp_005418"
     CDS             <1..292
                     /gene="tnpB"
                     /locus_tag="ACHKNP_RS27090"
                     /old_locus_tag="pgaptmp_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4707353.1"
                     /translation="RNGFNGLASKVQNVLKDDPFSGHLFIFRGRRGDQIKVLWADSDG
                     LCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINWKHPKRTERAGIRI"
     gene            312..>1417
                     /locus_tag="ACHKNP_RS27095"
                     /old_locus_tag="pgaptmp_005419"
     CDS             312..>1417
                     /locus_tag="ACHKNP_RS27095"
                     /old_locus_tag="pgaptmp_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HGU4707354.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTYITTEALQRIG"
BASE COUNT          330 a          405 c          405 g          277 t
ORIGIN      
        1 gcgaaatggc tttaacggcc tggcatcaaa agttcagaac gtcctgaagg atgacccgtt
       61 ctccggacac ctgttcatct tccgcggacg ccggggtgac cagataaaag tgttgtgggc
      121 tgacagtgac ggactgtgcc tcttcaccaa acgcctggag cggggccgct tcgtctggcc
      181 agtcacccgt gacggcaagg tgcaccttac tccggctcag ttatccatgc ttcttgaagg
      241 tatcaactgg aagcacccga aacgaacgga acgcgctgga atccgcatat aacccgttgt
      301 aaagtgagga tatggacacc tcacttgctc atgagaacgc ccgcctgcgg gcactgttgc
      361 agacgcaaca ggacaccatc cgccagatgg ctgaatacaa ccgcctgctc tcacagcggg
      421 tggcggctta tgcttccgaa atcaaccggc tgaaggcgct ggttgcgaaa ctgcaacgta
      481 tgcagttcgg taaaagctca gaaaaacttc gtgcaaaaac cgaacggcag atacaggaag
      541 cacaggagcg aatcagcgca cttcaggaag aaatggcgga aacgctgggt gagcaatatg
      601 acccggtact gccatccgcc ctgcgccagt cttcagcccg taaaccgtta ccggcctcac
      661 ttccccgtga aacccgggtt atccggccgg aagaggaatg ctgtcctgcc tgtggtggtg
      721 aactcagttc tctgggatgt gatgtgtcag agcaactgga gcttatcagc agcgccttta
      781 aggttatcga aacacaacgt ccgaaacagg cctgttgccg gtgcgaccat atcgtgcagg
      841 caccagtacc ttcaaaaccc attgcacgca gttatgccgg agcggggctt ctggcccatg
      901 ttgtcaccgg gaaatatgca gaccatctgc cgttataccg ccagtcagaa atataccgtc
      961 gtcagggagt ggagctgagc cgtgccacac tggggcgctg gacaggtgct gttgctgaac
     1021 tgctggagcc gctgtatgac gtcctgcgcc agtatgtgct gatgcccggt aaagtccatg
     1081 ctgatgatat ccccgtcccg gtccaggagc cgggcagcgg taaaacccgg acagcccggc
     1141 tgtgggtcta cgtccgtgat gaccgtaacg ccggttcaca gatgcccccg gcggtctggt
     1201 tcgcgtacag tccggaccgg aaaggtatcc atccacaaaa tcacctggcc ggttacagcg
     1261 gtgtgcttca ggccgatgct tacggtggtt accgggcgtt atacgaatcc ggcagaataa
     1321 cggaagccgc gtgtatggct catgcccgga gaaaaatcca cgatgtgcat gcaagagcgc
     1381 ccacctacat caccacggaa gccctgcagc gtatcgg
//
LOCUS       DAZFWL020000388         1387 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFWL020000388 DAZFWL020000000
VERSION     DAZFWL020000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.394"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            131..523
                     /gene="ydeI"
                     /locus_tag="ACHKNP_RS27100"
                     /old_locus_tag="pgaptmp_005420"
     CDS             131..523
                     /gene="ydeI"
                     /locus_tag="ACHKNP_RS27100"
                     /old_locus_tag="pgaptmp_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HGU4707355.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            778..>1387
                     /gene="dgcZ"
                     /locus_tag="ACHKNP_RS27105"
                     /old_locus_tag="pgaptmp_005421"
     CDS             778..>1387
                     /gene="dgcZ"
                     /locus_tag="ACHKNP_RS27105"
                     /old_locus_tag="pgaptmp_005421"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HGU4707356.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDSFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVY"
BASE COUNT          387 a          267 c          321 g          412 t
ORIGIN      
        1 tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa ttggcagtat
       61 tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca aacgcaatta
      121 aggagttagt atgaaatttc aggccattgt attagcaagt tttcttgtca tgccttatgc
      181 gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc ctcatgcaat
      241 agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt tcgcaaaaaa
      301 catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca aaggagagga
      361 ccgttacgtt tttcgcgata agagcggtga aattaatgtc gttattcctg cggccgtctt
      421 tgatggacga gaagttcagc cggaccagat gatcaacatt agcggcagtc tggataagaa
      481 aagcgcgccg gccgtcgttc gggtcactca tttgcagaaa taaaaataaa ttagcctgat
      541 ggcctgaact actgggccat tagggtattg ggttatgcta tttgtcatcc ttttatttac
      601 gataatgcag aggtgattta cagaattaaa cactttttta tccgctgatt ttgttttaaa
      661 tcacaatgtt atctcagaga tgactcataa cttcggattt ctatgaaaat attgacgtaa
      721 ttttgctttt ctctcgttag aatagcgcgc acaaggaact gtgaaaaagg agtggcaatg
      781 atcaagaaaa caacggaaat tgatgccatc ttgttaaatc tcaataaggc tatcgatgcc
      841 cactaccagt ggctggtgag tatgtttcac agcgtggtcg cgagagatgc cagtaagcca
      901 gaaataacgg ataaccattc ttatggactg tgccagtttg gtcggtggat tgatcatctg
      961 gggccactcg ataacgatga attaccttac gttcggctaa tggattctgc ccatcaacat
     1021 atgcataact gtggtcggga attaatgctg gctattgttg aaaatcactg gcaggacgcg
     1081 catttcgact cctttcagga ggggttgctt tcttttactg cggcattaac cgattacaaa
     1141 atttatttgc tgacgatccg tagcaatatg gatgttttga cgggattgcc ggggcgtcgg
     1201 gttcttgatg aatcctttga tcatcagtta cgcaacactg agcctctgaa tctttattta
     1261 atgttgttgg atattgaccg atttaaattg gttaatgata cctacgggca tttaatcggc
     1321 gatgtagtat tacgcaccct ggcaacttac ttagccagtt ggacgcgtga ttacgaaacg
     1381 gtttatc
//
LOCUS       DAZFWL020000389         1368 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFWL020000389 DAZFWL020000000
VERSION     DAZFWL020000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.395"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1196
                     /gene="gltS"
                     /locus_tag="ACHKNP_RS27110"
                     /old_locus_tag="pgaptmp_005422"
     CDS             <1..1196
                     /gene="gltS"
                     /locus_tag="ACHKNP_RS27110"
                     /old_locus_tag="pgaptmp_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGU4707357.1"
                     /translation="ASYTLLVACIALLIGMFVVKFTPFLQKNHIPEAVVGGFIVAIVL
                     LIIDKTSGYSFTFDASLQSLLMLTFFSSIGLSSDFSRLIKGGKPLVLLTIAVTILIAI
                     QNTVGMSMAVMMNESPFIGLIAGSITLTGGHGNAGAWGPILADKYGVTGAVELAMACA
                     TLGLVLGGLVGGPVARHLLKKVSIPKTTEQERDTIVEAFEQPSVKRKINANNVIETIS
                     MLIICIVVGGYISALFKDTFLQLPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVDVLGS
                     VALSLFLAMALMSLKFGQLASMAGPVLIIIAVQTVVMVLFACFVTFKMMGKDYDAVVI
                     SAGHCGFGMGATPTAIANMQTVTKAFGPSHKAFLVVPMVGAFIVDISNSILIKIFIEI
                     GTYFT"
BASE COUNT          369 a          264 c          274 g          461 t
ORIGIN      
        1 atgccagtta tacattatta gtcgcatgta tcgcgctact cataggaatg tttgtcgtaa
       61 aatttacccc gttcctacaa aaaaaccaca taccagaagc cgtcgttggt ggctttattg
      121 ttgcaattgt tctgttaatt attgataaaa catcaggtta ttcgtttact tttgatgctt
      181 cattgcaaag tttattaatg ctcacattct tttcctctat cgggctaagt tctgactttt
      241 ctcgactgat taaaggagga aagccgttag ttctattaac tattgcagta acgatcctaa
      301 tcgccattca aaatactgtc ggcatgagta tggctgtcat gatgaatgaa agtccattta
      361 ttggcttaat tgcaggttca attactctaa caggtggtca tggtaatgcc ggagcatggg
      421 gccctattct cgctgataaa tatggtgtaa caggcgccgt tgaattagcg atggcttgtg
      481 caacacttgg attagtgttg ggtggtttag ttggcggccc tgttgcccgt catcttttga
      541 aaaaggtctc tattcctaaa acaaccgagc aagagcgcga cactatcgtt gaagcttttg
      601 agcaaccaag cgtcaaaaga aaaatcaatg caaataacgt tattgaaacc atttcaatgc
      661 tgattatctg tattgttgtt ggtggctata tcagtgcatt gtttaaagat acttttctgc
      721 aactgcctac ttttgtctgg tgtttatttg tcggtattat tatccgtaat acactgactc
      781 atgtatttaa acacgaagtg tttgagccga ccgtcgatgt attaggtagc gttgctttat
      841 cgcttttctt ggcaatggcg ttaatgtcat taaaatttgg tcaattggca agcatggcag
      901 ggccagtatt aattatcatt gctgtacaaa ctgttgtcat ggtgctattt gcctgctttg
      961 tcaccttcaa aatgatgggc aaagattatg atgctgtcgt gatcagcgcg ggtcactgtg
     1021 gctttggtat gggagcaaca ccaacagcaa ttgcgaatat gcaaacagtc acaaaagcat
     1081 ttggaccatc acataaagct ttccttgtcg ttcctatggt cggtgccttt attgttgata
     1141 tttcaaacag tatcttaatt aaaatattta ttgaaattgg tacatacttt acctaatagc
     1201 tacacactac tcggcgtact tctcctaaca atttattata gggcagtatg ccgagccgct
     1261 ttcttacccc gtatattccc ctagtcaatc tatttagctt gcacgtttac gaattaattc
     1321 tcgtgacaac taaaccaaaa tttgaattat gacgagtata tctgatta
//
LOCUS       DAZFWL020000390         1356 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFWL020000390 DAZFWL020000000
VERSION     DAZFWL020000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.396"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..75
                     /locus_tag="ACHKNP_RS27115"
                     /old_locus_tag="pgaptmp_005423"
     CDS             <1..75
                     /locus_tag="ACHKNP_RS27115"
                     /old_locus_tag="pgaptmp_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4707358.1"
                     /translation="RMAEHIRYMVETIAHHQVDIDSEV"
     gene            81..866
                     /gene="bet"
                     /locus_tag="ACHKNP_RS27120"
                     /old_locus_tag="pgaptmp_005424"
     CDS             81..866
                     /gene="bet"
                     /locus_tag="ACHKNP_RS27120"
                     /old_locus_tag="pgaptmp_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGU4707359.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDDWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            863..>1356
                     /locus_tag="ACHKNP_RS27125"
                     /old_locus_tag="pgaptmp_005425"
     CDS             863..>1356
                     /locus_tag="ACHKNP_RS27125"
                     /old_locus_tag="pgaptmp_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HGU4707360.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVT"
BASE COUNT          357 a          349 c          360 g          290 t
ORIGIN      
        1 cgcatggcag aacacatccg gtacatggtt gaaaccattg ctcaccacca ggttgatatt
       61 gattcagagg tataaaacgg atgagtacag cactcgcaac gctggcaggg aagctggctg
      121 aacgtgtcgg catggattct gtcgacccac aggaactgat caccactctt cgccagacgg
      181 catttaaagg tgatgccagc gatgcgcagt tcatcgcatt gttgatcgtc gccaaccagt
      241 acggccttaa tccgtggacg aaagaaattt acgccttccc tgataagcag aacggcattg
      301 ttccggtggt gggcgttgat gactggtccc gcatcatcaa tgaaaaccag cagtttgatg
      361 gcatggactt tgagcaggac aatgaatcct gtacatgccg gatttaccgc aaggaccgta
      421 atcatccgat ctgcgttacc gaatggatgg atgaatgccg ccgcgaacca ttcaaaaccc
      481 gcgaaggcag agaaatcacg gggccgtggc agtcgcatcc caaacggatg ttacggcata
      541 aagccatgat tcagtgtgcc cgtctggcct tcggatttgc tggtatctat gacaaggatg
      601 aagccgagcg cattgtcgaa aatactgcat acactgcaga acgtcagcca gaacgcgaca
      661 tcactccggt taacgatgaa accatgcagg agattaacac tctgctgatc gccctggata
      721 aaacatggga tgacgactta ttgccgctct gttcccagat atttcgccgc gacattcgcg
      781 catcgtcaga actgacacag gccgaagcag tgaaagctct tggattcctg aaacagaaag
      841 ccactgagca gaaggtggca gcatgatacc ggacattatc ctgcagcgta ccgggatcga
      901 cgtgagagct gtcgaacagg gggatgatgc atggcacaaa ttacggctcg gcgtcatcac
      961 cgcttcagaa gttcacaacg tgatagcaaa gccccgctca ggaaagaagt ggcctgacat
     1021 gaaaatgtcc tacttccaca ccctgctggc tgaggtttgc accggtgtgg ctccggaagt
     1081 taatgctaag gcgctggcct ggggaaaaca gtacgagaac gacgccagaa ccctgtttga
     1141 attcacttcc ggcgtgaata ttactgaatc cccgatcatc tatcgcgacg aaagtatgcg
     1201 caccgcctgc tctcccgatg gtttatgcag tgacggcaac ggccttgaac tgaaatgccc
     1261 gtttacctcc cgggatttca tgaaattccg gctcggtggt ttcgaggcaa taaaatcggc
     1321 ttacatggcc caggtgcagt acagcatgtg ggtgac
//
LOCUS       DAZFWL020000391         1354 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFWL020000391 DAZFWL020000000
VERSION     DAZFWL020000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.397"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            41..1186
                     /locus_tag="ACHKNP_RS27130"
                     /old_locus_tag="pgaptmp_005426"
     CDS             41..1186
                     /locus_tag="ACHKNP_RS27130"
                     /old_locus_tag="pgaptmp_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EspG domain-containing protein"
                     /protein_id="HGU4707361.1"
                     /translation="MINGLNNNSASLVLDAAIRINSDFKKQWNDMSCAEKLLKVLSFG
                     LWNPTYTRSERQTFQELLTVLEPVSPAPNELGRIYANFADGSSLRISVTNSELVEAEI
                     RTPDNEKILMLLESNEQNRLLQSLPINLHMPYIQVHRALSKMDLTDHKSMHNLLSFTS
                     KLSATLIPHNTQTDPLSGPTPFSSMFMDTFRGLGNAKLSLNGVDIPVDAQKLLRDALG
                     LKDTHSSLARNVINNGISRHHAKQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALMLTHTRISQAREYNVERSLDVPNACINISISQSPDGSIHVASHTGILIMAP
                     EDRPNELGMLTNRTSYEVPQGVKCEIDEMVRTLQPRYGASETYLKNI"
BASE COUNT          463 a          280 c          244 g          367 t
ORIGIN      
        1 tgtcgaatca ttcataataa atgagacagg ggaatctctc atgataaacg gacttaataa
       61 taattccgca tctttagttt tagatgctgc aattagaatt aattctgatt ttaaaaagca
      121 gtggaatgat atgtcatgcg ctgaaaaatt actaaaagtt cttagttttg gtttatggaa
      181 tccaacgtat actcgtagtg aaaggcaaac atttcaagag ttgttaaccg ttttagaacc
      241 tgtatctccc gcgcctaatg aattgggcag aatatatgct aattttgcag atggctcatc
      301 cttaagaatt tccgtcacta acagcgaact tgttgaagcc gagattcgca caccagataa
      361 tgaaaagatt cttatgctcc tggagtcaaa cgaacaaaat aggttattac aatctttacc
      421 catcaatctc cacatgccat acattcaggt tcatcgtgcc ttatctaaga tggacctgac
      481 tgatcataag tcaatgcaca atctacttag ttttacgtca aaactatcag caaccttgat
      541 tcctcataat actcaaacag atccgctttc cgggcctaca ccattcagct ctatgtttat
      601 ggatacattt cgaggactcg gcaatgcaaa gctttcactc aatggtgttg atatacctgt
      661 agatgcacaa aaattgcttc gcgatgcact aggacttaaa gacacacatt catcacttgc
      721 ccggaatgtt ataaataatg gtatttctcg tcatcatgca aagcaaatag caagggaaag
      781 cagcggcagc gataaacaga aagctgaagt tgtggagttt ttatgccacc ccgaagcggc
      841 aacagccata tgctcggctt tctatcaatc tttcaatgtg ccagccttaa tgttgacaca
      901 tacaaggatc tctcaagcca gagaatacaa tgtggaaaga tcgttagatg tacctaacgc
      961 gtgcattaac ataagtatct ctcaatcacc agatggaagc attcacgttg ccagccatac
     1021 cgggattctg ataatggcgc cagaagaccg ccccaacgag ttaggaatgt tgacgaacag
     1081 gacctcctat gaagtgccac aaggtgtgaa atgtgaaatc gatgagatgg taagaacgct
     1141 acaaccaagg tatggcgcat ctgaaacata cctgaaaaac atttaaaaat aaaaaaatgg
     1201 acaccacaca ctttatttat caaaacaaac aataactaat cacactcaca attattaata
     1261 atttaacctc acagcaatca tacaaaacaa agatatttat ttcgcaaacc atttatagcg
     1321 aatggagaag gaatgaaaag aatcttattt attg
//
LOCUS       DAZFWL020000392         1353 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFWL020000392 DAZFWL020000000
VERSION     DAZFWL020000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.398"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /locus_tag="ACHKNP_RS27135"
                     /old_locus_tag="pgaptmp_005427"
     CDS             <1..198
                     /locus_tag="ACHKNP_RS27135"
                     /old_locus_tag="pgaptmp_005427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707362.1"
                     /translation="FNNHDGNVIIVNTSSRFELRKKYVGTLKINSGVSTVDPHWPPVS
                     KRIKLIFFSPTLHSFSCIGVS"
     gene            234..>1353
                     /locus_tag="ACHKNP_RS27140"
                     /old_locus_tag="pgaptmp_005428"
     CDS             234..>1353
                     /locus_tag="ACHKNP_RS27140"
                     /old_locus_tag="pgaptmp_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707363.1"
                     /translation="MSRRKVGNMGRGDFDRLCNASGLIYNSSSEDDSGGWDAIVEFPL
                     LGGIALLNSLNEQPIQCLVQIKSSDGEKKGVQVKLSNMKRFCDTPLPCFFFFARYNKN
                     ANIESAYLVHINSDIMFQVLKRIRQNDIGKKSPLHKLSFTVNYSDEDEIDLTNEYALK
                     NAIEKHIPHGMKKYSEDKLRDLGNVGYNESRYAFNFKVDNNEDYKSLISATLGYPTRI
                     NVKDVRGWDTRFDINFQMEELTAEQAIIEIRSVEAFSTGSIIFEDEKEDVSFKCEFFV
                     SPIALNAPRDLASFRICSDFFDLLVGIKNNKLKIDFESYDKPFHLADIRELLLLMKIL
                     NAPSGDLKLTIKNNEGNETSFLASDNSFSANPITNEIIK"
BASE COUNT          446 a          217 c          272 g          418 t
ORIGIN      
        1 tttaataatc atgatggtaa tgtgattatt gtgaatacga gctcaaggtt tgagctccgg
       61 aaaaaatacg ttgggacgtt aaaaatcaac tcgggagtat ccacagttga tcctcattgg
      121 ccccctgtaa gcaagcgcat caagctaata tttttcagcc cgacacttca tagtttttcc
      181 tgcattgggg taagctaaac ctagtatcgt acatatgaaa gagggatgag tttatgagtc
      241 gaagaaaagt tggtaatatg ggacgtggtg attttgacag gctttgcaat gcatctggct
      301 tgatttacaa cagctcctct gaggatgatt ccggagggtg ggatgccatt gtcgaatttc
      361 ctctgttagg tggtatagcc ttactgaata gtttgaatga acagcctata caatgtttag
      421 tgcagataaa gtcttctgac ggagaaaaga aaggtgtaca ggtcaaactt tctaacatga
      481 aacgtttctg tgacacacct ctaccctgtt ttttcttttt tgcgcgttat aataagaatg
      541 ctaatatcga atcagcttac cttgtccaca ttaatagtga tatcatgttt caggttctga
      601 agcggattcg tcaaaatgat ataggaaaaa aatcaccact tcacaaactt tctttcacgg
      661 taaattactc tgatgaggat gagatagacc tgactaatga atatgcactc aaaaatgcta
      721 ttgaaaaaca tattcctcat ggaatgaaaa aatatagtga agataaattg agagatttag
      781 gtaatgtagg atataacgaa agccgctatg cattcaattt caaagttgat aataatgagg
      841 attataagtc tcttattagt gccacgcttg gatatccgac cagaataaat gtaaaagatg
      901 ttcgcggttg ggatactcga tttgatatta attttcagat ggaagaactg acagctgaac
      961 aagcaataat cgaaataaga tctgttgaag cattctcaac aggctctata atattcgaag
     1021 atgaaaaaga agatgtctct tttaagtgtg aatttttcgt ttctcctatt gcacttaatg
     1081 ctccaaggga tctggcaagc tttagaattt gctcggactt tttcgattta ctggttggta
     1141 ttaaaaataa caaattgaaa atagattttg aatcatatga taagcctttt catttagctg
     1201 acattagaga attactgtta ttaatgaaga tattaaatgc cccaagcgga gatctgaagt
     1261 taacaataaa aaacaatgag ggtaacgaga catccttttt agcctcggat aattcatttt
     1321 ctgcgaatcc aattactaac gaaataataa agg
//
LOCUS       DAZFWL020000393         1352 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFWL020000393 DAZFWL020000000
VERSION     DAZFWL020000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.399"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(152..496)
                     /locus_tag="ACHKNP_RS27145"
                     /old_locus_tag="pgaptmp_005429"
     CDS             complement(152..496)
                     /locus_tag="ACHKNP_RS27145"
                     /old_locus_tag="pgaptmp_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707364.1"
                     /translation="MSDLSMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEKIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(525..692)
                     /locus_tag="ACHKNP_RS27150"
                     /old_locus_tag="pgaptmp_005430"
     CDS             complement(525..692)
                     /locus_tag="ACHKNP_RS27150"
                     /old_locus_tag="pgaptmp_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707365.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(765..1049)
                     /locus_tag="ACHKNP_RS27155"
                     /old_locus_tag="pgaptmp_005431"
     CDS             complement(765..1049)
                     /locus_tag="ACHKNP_RS27155"
                     /old_locus_tag="pgaptmp_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGU4707366.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKVNIGNE"
     gene            complement(1042..1185)
                     /locus_tag="ACHKNP_RS27160"
                     /old_locus_tag="pgaptmp_005432"
     CDS             complement(1042..1185)
                     /locus_tag="ACHKNP_RS27160"
                     /old_locus_tag="pgaptmp_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707367.1"
                     /translation="MKVWVYDPETILNVEERRRTEVYIHNLNEDGFAPVLVKATPQEV
                     NRG"
     gene            complement(1195..1344)
                     /locus_tag="ACHKNP_RS27165"
                     /old_locus_tag="pgaptmp_005433"
                     /pseudo
     CDS             complement(1195..1344)
                     /locus_tag="ACHKNP_RS27165"
                     /old_locus_tag="pgaptmp_005433"
                     /inference="COORDINATES: protein motif:HMM:TIGR03655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
BASE COUNT          331 a          327 c          285 g          409 t
ORIGIN      
        1 tgggcgtcgc tggcgtgcat tccactcctg aagtgtcaag tacatcgcaa agtctccgca
       61 attacacgca agaaaagccg cattgatgcg gcgatggtag gtctggatat cattgagcca
      121 tgaacaggcc tcatcgagtg tgaggctgtg gttagtcctt tcgtaactcg ctgattctcc
      181 tgtaagtctc tggtgctttg tttccgtgta tcttcatttc agacttcaac agagcaacga
      241 gggaatccca ttcgttgagg atgcctttga atgccggaac gcgctttgca accttgtcga
      301 atgaatctct gatttctgga atcttctcaa caagtgcaac gcatcgtcgg aagtctgctg
      361 cgtcatgggg agcgccgaag tgatgaccat agatattctt tttcaggcca catgcgattg
      421 aggcaagagt tgcgctactg atgccaacat cgccagtcga ttgccatttc aaaaccttca
      481 tagacaaatc tgacatttct tgtctccaat aaaaaaaacc tccatcaggc ggcttggtgt
      541 tctttcagtt cttcaattcg aatattggtt acgtctgcat gtgctatctg cgcccatatc
      601 atccagtggt catagcagtc attgatgttc tctgcttcga taactctgtt gaatggttct
      661 ccattccatt cacctgtgac tcggaagtgc atttatcatc tccataaaac aaaatccgcc
      721 gtagcgagtt cagataaaag aaatccccgc gagtgcgagg attgttattc attgccgata
      781 ttcaccttta tcgcgaacac ctttaccggt ttatcaccga agtgcggatg tgtgattgtc
      841 ttgatttcat atccgtcata cggaacatca attctgcggc tggaatcgtc gcgcttcgga
      901 tatccctttg tgataatcag gcggtcatac tcccggaaca taattcgctt attccaatag
      961 tcattacaca ggcgatactc ttccgttttc tctcctcgaa tcatggcatc gaagtattca
     1021 cctttgacgg caagttgtag gttagccacg gttaacctcc tgcggagtag cctttacaag
     1081 cactggtgca aatccatctt cattaaggtt atgaatatag acttctgttc tcctgcgctc
     1141 ttcaacgttt aatattgttt ctggatcata aacccatact ttcattcgac tattccatga
     1201 agaaatcgct tcagatctag ttcgtttttc tggtccttgg gcaccacatt tgcatgacac
     1261 atagcgcatt ttccctctga tactgaatga gtatccgatg ttaagcacag tggaaccaca
     1321 gaatgggcag cgatatagat tcataaatca tc
//
LOCUS       DAZFWL020000394         1330 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFWL020000394 DAZFWL020000000
VERSION     DAZFWL020000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.400"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..110
                     /locus_tag="ACHKNP_RS27170"
                     /old_locus_tag="pgaptmp_005434"
     CDS             <1..110
                     /locus_tag="ACHKNP_RS27170"
                     /old_locus_tag="pgaptmp_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HGU4707368.1"
                     /translation="KGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            110..355
                     /locus_tag="ACHKNP_RS27175"
                     /old_locus_tag="pgaptmp_005435"
     CDS             110..355
                     /locus_tag="ACHKNP_RS27175"
                     /old_locus_tag="pgaptmp_005435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707369.1"
                     /translation="MKHIPEGVIQVPESPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     EISLCRLSTGNDIWPSVCDRNGYAYGCPEFQPNIIHP"
     gene            449..922
                     /locus_tag="ACHKNP_RS27180"
                     /old_locus_tag="pgaptmp_005436"
     CDS             449..922
                     /locus_tag="ACHKNP_RS27180"
                     /old_locus_tag="pgaptmp_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4707370.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYSLSNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            938..>1330
                     /locus_tag="ACHKNP_RS27185"
                     /old_locus_tag="pgaptmp_005437"
     CDS             938..>1330
                     /locus_tag="ACHKNP_RS27185"
                     /old_locus_tag="pgaptmp_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HGU4707371.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQAL"
BASE COUNT          299 a          387 c          354 g          290 t
ORIGIN      
        1 caaaaggaaa acgtatccac acccgtgcca ttcacagcat cgacaccgat attaagctca
       61 accgcgcatt gtgggtgatg gcagaaacgc tgctggagag tatgcgctga tgaaacatat
      121 ccctgaaggc gtgattcagg taccagaatc ccccgacgcc ccggaagcgc tgccggggtg
      181 tcgttccatt tctgatgatg acctgtgcgc ctggtgtacc cgtctgcttt accgtcccgg
      241 agaaatcagt ctgtgcagac tgagtacagg caatgacatc tggccatccg tctgtgaccg
      301 gaacggttac gcgtacggct gtccggaatt tcagccgaat atcattcatc cctgatatta
      361 ccgggtctca cggtcagtga tgaacgtgat gttgccagct cccgaccttt actccctctc
      421 atttattcac attacaagga tttcatacat gaaaacattg tctcagaaca ccacctcttc
      481 ggcctgtgca ccggagactg gcctgcaaca actggttgcc accatagtcc ctgatgaaca
      541 gcgcatcagc ttctggccgc agcactttgg tctcattcca cagtgggtga ccctggagcc
      601 ccgtgtcttc ggctggatgg accgtctgtg cgaagactac tgcggtggta tctggaatct
      661 gtacagtctg agcaacggcg gggcatttat ggcccccgaa ccggatgacg atgatgacga
      721 aacatgggta ctgttcaatg ccatgaacgg taaccgcgct gaaatgagcc cggaagccgc
      781 tggcattgct gcctgtctga tgacgtacag tcatcacgcc tgtcgtacgg agtgttatgc
      841 catgacggtc cattattacc ggctgcggga ttacgccctg cagcatccgg aatgcagcgc
      901 cattatgcgc atcatcgact gaatggaggc agggacaatg cagcagcttt cctttctgcc
      961 cggagaaatg acgcccggcg agcgcagtct cattctgcgg gctctgaaaa ccctggaccg
     1021 ccatcttcat gaacccggtg tggccttcac ctccacccgt gcggcacggg aatggctgat
     1081 tctgaacatg gcgggactgg agcgtgaaga gttccgggtg ctgtatctga ataaccagaa
     1141 tcagctgatt gccggtgaaa ccctcttcac cggcaccatc aaccgcacgg aagtccatcc
     1201 ccgggaagtg attaaacgcg ccctgtacca caatgccgct gccgtggtgc tggcgcacaa
     1261 tcacccgtcc ggtgaagtca cacccagcaa ggcagaccgc cttatcacgg aacgtctggt
     1321 acaggcactg
//
LOCUS       DAZFWL020000395         1330 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFWL020000395 DAZFWL020000000
VERSION     DAZFWL020000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.401"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..137)
                     /locus_tag="ACHKNP_RS27190"
                     /old_locus_tag="pgaptmp_005438"
     CDS             complement(<1..137)
                     /locus_tag="ACHKNP_RS27190"
                     /old_locus_tag="pgaptmp_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4707372.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SG"
     gene            469..>1330
                     /locus_tag="ACHKNP_RS27195"
                     /old_locus_tag="pgaptmp_005439"
     CDS             469..>1330
                     /locus_tag="ACHKNP_RS27195"
                     /old_locus_tag="pgaptmp_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707373.1"
                     /translation="MDMIPSYESMYLTLRGIPTDSIGGEDIYFIHDPQGGWYPNRGNH
                     LYAIDALCVNIHDALANSIFDGLENYHKFLYMAPEFLSTAGLNSESVVSKETFVLFID
                     KFKGHTDVNKGLYLFDCCKIVSSIQECSKEVLQLQGEFYYTLNFEPLFFPNIEEEDGI
                     RYVTSPVVTKLFALLGFIYIRMYSLLDYITKLAIEIENLKTQFSSYVKLTSKNSQYGD
                     KKKVSLNNYKGSLFEQCPFINEIESVRNHIIHDGLLDDMPKAYRVIRNNACIEKYLLF
                     PDQTSEGRFES"
BASE COUNT          431 a          229 c          271 g          399 t
ORIGIN      
        1 ccgcttttac ccataatgcc aatcacgggt tcgtgactgg tgagtttgcg cagatgttcc
       61 aggatgtgac gggaaagtga gtaaggcagg gaggagagcg gtttttcaat tgcctcaatg
      121 gcatcagacg gattcataca tgtattcccg gtgaaaaaag acaaaacccc gcgcaccgga
      181 aaggtggcgg gggattcaca acgataatat ctgtctgaaa ttatttggaa tgcaggtata
      241 tgtggctgat ttgtatcagc taatgagtac aggtctgaat ctaatctgac agtccgctct
      301 gtgccagaag cgggcgttag gttgagtaaa gaaaagccca taaaattccc tgcgtttgat
      361 aaacccggat atgaaaaatt attactttca gagtatgatt atcttgctta aattaaccta
      421 tatacgaaac ctcacttttt cgattcataa gaggaattaa agaaaaacat ggatatgata
      481 ccttcatatg aaagcatgta tttgacttta agagggatac caactgactc aattggaggt
      541 gaggacattt actttataca tgatcctcaa ggtggatggt acccgaacag agggaatcat
      601 ctttatgcga tcgatgcatt atgcgttaat attcatgatg ctctagctaa tagcattttt
      661 gatggattag aaaactacca caagttcctt tatatggccc ctgagttttt atcaacagca
      721 ggtttgaact ctgaatccgt agtttcaaaa gaaacatttg ttttatttat tgataaattc
      781 aaagggcata ctgacgtaaa taaaggcctt tacctttttg attgctgtaa aattgtaagt
      841 agcatacagg aatgcagcaa ggaagtgttg caactacaag gggagtttta ttacacgttg
      901 aattttgaac ccttgttttt ccctaacatt gaagaggagg acggtataag atatgttaca
      961 tctccagtcg tgactaagct ctttgcatta cttggattta tttatatccg catgtacagt
     1021 ttgctcgact acataacaaa actagctata gaaattgaaa atttaaaaac acagttctct
     1081 tcttatgtta aactcacatc caagaactct caatatggtg ataaaaagaa agtgagctta
     1141 aataattata aaggatcttt gtttgagcaa tgcccattta ttaatgaaat agaaagtgta
     1201 cgaaaccata tcatacatga tggtctttta gacgatatgc caaaagcata cagagttatc
     1261 cggaacaacg catgtattga aaaatatttg cttttccctg accaaacatc agaaggacgt
     1321 tttgaatcgt
//
LOCUS       DAZFWL020000396         1325 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFWL020000396 DAZFWL020000000
VERSION     DAZFWL020000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.402"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..212
                     /locus_tag="ACHKNP_RS27200"
                     /old_locus_tag="pgaptmp_005440"
     CDS             <1..212
                     /locus_tag="ACHKNP_RS27200"
                     /old_locus_tag="pgaptmp_005440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707374.1"
                     /translation="ADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILA
                     GISAGGMYCPECGTVHWPDGVPPPF"
     gene            212..>1325
                     /locus_tag="ACHKNP_RS27205"
                     /old_locus_tag="pgaptmp_005441"
     CDS             212..>1325
                     /locus_tag="ACHKNP_RS27205"
                     /old_locus_tag="pgaptmp_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HGU4707375.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCSHIKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRRLLAEMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQEWHPHI
                     HLSTTTGGVTSDHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAAEGSSKR
                     DWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVYVITE
                     TVRKTAMQIRWRGMYQRLLKVDPLKCILCGGQMRFTG"
BASE COUNT          296 a          318 c          412 g          299 t
ORIGIN      
        1 gcgcagacct gaatattccc ttctttgtgg atgccggaaa caaaaagctg gtcttctggt
       61 ttgtacgtgt tgatgacgaa gggtatcctg aaatagcccg ctgcacggag cgggagtttg
      121 cgaccattct tgccggtatc agcgccggcg gcatgtactg cccggagtgt ggcacggttc
      181 actggccgga cggagtcccc ccgcccttct gatgcttccc cgttttgccg acatttttca
      241 gcagggaaac cgctggctta actggctgga gaaacaaccg gaaggttcag tgcgtccggt
      301 agtcattgag tctgtgacaa aaatcatggc ctgcgggacc acgctgatgg ggtacacaca
      361 gtggtgctgt tcatctccgg actgcagcca cataaaaaag gtctgcttcc ggtgtaaaag
      421 tcgctcctgc ccgcactgcg gagtgaaggc tggcgcacag tggatacagt atctgctgag
      481 tctggttccc gactgtccgt ggcagcatat tgtgttcaca cttccctgcc agtactggtc
      541 cctggtgttc cacaaccgga ggttactggc agagatgagc cgcattgctg cggatgtgat
      601 acaggaaatc tgccgccagg cagatgtggt gccggggata ttcacggtga tccacacatg
      661 gggacgtgac caggagtggc atccgcacat tcacctgtcg acaacgaccg gcggcgtgac
      721 atcagaccac acctggaaaa accttcattt ttacgcccgt aaggtgatga gtatgtggcg
      781 ttaccggata acgcggttac tgtcacggaa atatccggac ctggtgatac cggatgcgct
      841 ggcagcagaa ggaagcagta aacgggactg gaatcgcttc ctggacagtc attaccggcg
      901 gggctggaat gtcaacgtat cccgggtgat ggataacgcc acacatgtgg cggtgtactt
      961 cggctcttac ctgaaaaaac cgccggtgcc gatgagccgt ctggagcact atgctggtca
     1021 ggatgaaatt ggtctgcgtt acaacagtca ccggacaaaa cgggaagaat acctggtgat
     1081 gagtggtgat gagtttatgg aaaggttctc ctggcatgtg gcggataagg ggttccgtat
     1141 ggtgaggtac tacggtttcc tgagtccggt gaagcgccgg ttactggaag atgttgtgta
     1201 cgtcataacg gagacggtga gaaagacggc gatgcaaatc aggtggagag ggatgtatca
     1261 gcggttactg aaggttgacc cgctgaagtg catcctgtgc ggaggtcaga tgcgttttac
     1321 ggggc
//
LOCUS       DAZFWL020000397         1307 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFWL020000397 DAZFWL020000000
VERSION     DAZFWL020000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.403"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            299..592
                     /gene="iss"
                     /locus_tag="ACHKNP_RS27210"
                     /old_locus_tag="pgaptmp_005442"
     CDS             299..592
                     /gene="iss"
                     /locus_tag="ACHKNP_RS27210"
                     /old_locus_tag="pgaptmp_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HGU4707376.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(683..865)
                     /gene="rzoD"
                     /locus_tag="ACHKNP_RS27215"
                     /old_locus_tag="pgaptmp_005443"
     CDS             complement(683..865)
                     /gene="rzoD"
                     /locus_tag="ACHKNP_RS27215"
                     /old_locus_tag="pgaptmp_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HGU4707377.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1082..>1307)
                     /gene="rrrD"
                     /locus_tag="ACHKNP_RS27220"
                     /old_locus_tag="pgaptmp_005444"
     CDS             complement(1082..>1307)
                     /gene="rrrD"
                     /locus_tag="ACHKNP_RS27220"
                     /old_locus_tag="pgaptmp_005444"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGU4707378.1"
                     /translation="VDIPETTRGALYSFVYNVGAGNFRTSTLLRKINQGDIKGACDQL
                     RRWTYAGGKQWKGLMTRREIEREICLWGQQ"
BASE COUNT          332 a          316 c          302 g          357 t
ORIGIN      
        1 tcagcacagt aaaacccggt gtgcatcgtt tttgattatt cccgcacact cacgcagaag
       61 gaattccccg tcgggctacg gtcatggtta atgcgggaat acggcgacga tacagcgcag
      121 ctaaaagggt aatggacaga tagagcggtt tatttcattc cacaggattc tgagtgcccc
      181 cctcctccaa taggctgagc atccacctat atagttttaa ttttcatcaa tccatttaac
      241 tatcgtttaa ttgttgtcac ataggattct gccgttttta acaatgcagg ataataagat
      301 gaaaaaaatg ttgttttctg ccgctctggc aatgcttatt acaggatgtg ctcaacagac
      361 gtttactgtt ggaaacaaac cgacagcagt aacaccaaag gaaaccatca cccatcactt
      421 cttcgtttcg ggaattggac aggagaaaac tgttgatgca gccaaaattt gtggcggcgc
      481 agaaaatgtt gttaaaacag aaacccagca aacattcgta aatggattgc tcggttttat
      541 tactttaggc atttatactc cgctggaagc gcgtgtgtat tgctcacaat aattgcatga
      601 gttgcccatc gatatgggca gctctatctg cactgctcat taatatacgt ctgggttcct
      661 tccagttgtt tttgcatagt gatcagcctc tctctgaggg tgaaataatc ccgttcagcg
      721 gtgtctgcca gtcgggggga ggctgcatta tccacgccgg aggcggtggt ggcttcacgc
      781 actgactgac agactgcttt gatgtgcaac cgacgacgac cagcggcaac atcatcacgc
      841 agagcatcat tttcagcttt cgcatcagct aactccttcg tgtattttgc atcgagcgca
      901 gcaacatcac gctgacgcat ctgcatgtca gtaattgccg cgttcgccag cttcagttct
      961 ctggcatttt tgtcgcgctg ggctttgtag gtaatggcgt tatcacggta atgattaaca
     1021 gcccatgaca ggcagacgat gatgcagata accagagcgg agataatcgc ggttactctg
     1081 ttcattgctg accccacaaa cagatttcac gctcaatctc acgacgagtc atgagacctt
     1141 tccattgctt accgccagca tatgtccagc gacgtagctg atcacatgcg cctttgatat
     1201 cgccctggtt tattttgcga agaagcgtcg atgttctgaa attgccagca cccacgttgt
     1261 aaacgaatga gtaaagagcg ccgcgcgttg tttccggtat atcgact
//
LOCUS       DAZFWL020000398         1306 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFWL020000398 DAZFWL020000000
VERSION     DAZFWL020000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.404"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..877)
                     /locus_tag="ACHKNP_RS27225"
                     /old_locus_tag="pgaptmp_005445"
     CDS             complement(<1..877)
                     /locus_tag="ACHKNP_RS27225"
                     /old_locus_tag="pgaptmp_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707379.1"
                     /translation="MLKSFCLNFDGMYLSIVSFLSEKFNLKRVGKMELESKEDYEETT
                     HHNVASSVVTATHDNLDGREFFELCSNPALVFSFETHKKFNELPVDSKESPLDLFIAN
                     TKQLHLLRSGDISNTLSHLLLLGYVSAAESYLRALIRNLINHDEYIQSIVSDKAVSYA
                     AAQHHKKELLPEALLEDFSLASPYNVFETLKDIIGMKGQRPIEMMKCSSEFMKVCELR
                     HCCVHRFGKLGSKNAIRLGLAEHMKHLEKPIILNNDDLEQIAFIVENFIRTLNNTVFK
                     FIINRTVENKNKEKGG"
     gene            complement(855..>1306)
                     /locus_tag="ACHKNP_RS27230"
                     /old_locus_tag="pgaptmp_005446"
     CDS             complement(855..>1306)
                     /locus_tag="ACHKNP_RS27230"
                     /old_locus_tag="pgaptmp_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU4707380.1"
                     /translation="VFNEGSRGFSPSQIIQKILRKNAKHPVRRNISSLFNAEQLARLK
                     LTLPPCNNCKTPRLTDNQKFCHHCGNQLIDESAYNQCMSIPLSKVPHLTSWQMQKLSG
                     LEKVKTIGDVLSLQDPGSELRKIHRIGPKIANKIMDKVLLHVEEFLS"
BASE COUNT          396 a          250 c          207 g          453 t
ORIGIN      
        1 cccctccttt ttccttattt ttgttctcaa ctgtacgatt aattataaat ttgaaaacgg
       61 tattattcaa agttctaata aaattctcaa caataaatgc gatttgctcg agatcatcat
      121 tattgagtat tatcggtttt tctaagtgct tcatatgttc agccaatcct agtcggattg
      181 catttttcga acccaactta ccaaatcgat gaacacaaca atgccttaac tcgcaaactt
      241 tcatgaattc gcttgagcat ttcatcattt cgattggtcg ttgtcctttc attccaataa
      301 tgtcctttaa tgtttcaaaa acattataag ggcttgccaa tgaaaagtcc tcaagtaaag
      361 cttctggtaa taactccttt ttatggtgtt gtgccgctgc ataactaacg gccttatcag
      421 aaactataga ctgtatatat tcatcatgat taattagatt tctgataaga gctcgcaaat
      481 aactttcggc tgcagacaca taacctaata acaacagatg gcttaatgtg tttgaaatat
      541 caccagatct taacaagtga agttgctttg tattagctat aaaaagatct aacggggatt
      601 ccttgctatc aacaggtaac tcattaaatt ttttgtgtgt ctcaaacgaa aaaactagag
      661 ctggattact acatagttca aaaaactctc ttccatctaa gttatcatgg gttgcagtga
      721 caactgagga cgccacatta tgatgagtgg tctcttcata atcctctttt gattctagtt
      781 ccattttccc gactcttttt aaattaaact tttcagataa gaaagacacg attgacaagt
      841 acatgccatc aaaattaaga caaaaactct tcaacatgta ataacacttt gtccataatt
      901 ttattggcaa ttttgggtcc tattctgtga atttttctta actcactccc agggtcttgt
      961 aaagacaata catctcctat agtttttact ttttccagcc cagatagttt ttgcatttgc
     1021 caagatgtta gatgtggtac ttttgacagt ggaatagaca tgcactgatt ataggccgac
     1081 tcatcaatca attgattccc acaatgatgg caaaattttt gattgtcagt caatcgcgga
     1141 gtcttgcagt tattgcatgg aggtaaagtt agtttaagtc tcgcaagttg ctcagcattg
     1201 aataatgatg aaatgtttct tctgacagga tgcttagcat tctttcttag tattttttga
     1261 ataatctgag aaggagaaaa acctctactt ccttcgttaa acactc
//
LOCUS       DAZFWL020000399         1303 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFWL020000399 DAZFWL020000000
VERSION     DAZFWL020000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.405"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..211)
                     /locus_tag="ACHKNP_RS27235"
                     /old_locus_tag="pgaptmp_005447"
     CDS             complement(<1..211)
                     /locus_tag="ACHKNP_RS27235"
                     /old_locus_tag="pgaptmp_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707381.1"
                     /translation="MSTGLRFTLEVDGLPPNAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQR"
     gene            complement(1031..1243)
                     /locus_tag="ACHKNP_RS27240"
                     /old_locus_tag="pgaptmp_005448"
     CDS             complement(1031..1243)
                     /locus_tag="ACHKNP_RS27240"
                     /old_locus_tag="pgaptmp_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HGU4707382.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
BASE COUNT          441 a          254 c          243 g          365 t
ORIGIN      
        1 ggcgctgtac ttcatcgccc tgccatatcg tcaggtaggc cattttgtcc agcacctgcg
       61 caaattcaag ggagagaaac tgctggctga ccagggagag atcgagggaa aagagcgaag
      121 agagtgactg gttcagatga aaggaaacca ccgcaaaagc attcggcggc aggccgtcca
      181 cttccagtgt gaaacgtaat ccggttgaca tagcaacctc cgtgaaaatg aataataatc
      241 cgcacatatt gcatgtacaa catgtcgggc ggatgagata agcgagtcga catctggcgt
      301 taccggggaa tatcagaaaa atgtcatggt tatgtgatga tttagtctgg gattttactt
      361 cagagaaacg ggtgtctgtt tagtcaacaa aataatcaca aacgcaacgg atgggttctc
      421 gacgttattc acgacaggct cgtacattac agcgagtaat aaaaaacctg atatgcaaag
      481 tcaaaccaat attcataatg tctttttatt cacaacaata aataattcaa taacaatcat
      541 tgaaatgaat gatggtttgt ataaggaata tacgctaccg ccccaccctg ataacatgat
      601 ctgcacattg ccatcttaac gtcgttaaac actagcttaa tagtggatat ttcaaattag
      661 gaatttatta aattcacctg atgaatataa taagcaaata gcatagaaaa taatgacaac
      721 tcccccgagg ggaccatttc caaagcactg caccaccacc tggtgccgga tgatcttgct
      781 tcagtggtaa gagtacctga tgcattgttc ccaaaatggt gttttcccct gcccctccaa
      841 taatgtgtag ctctcacttg ataatctatt gattaggtta tattaagaat caacatttaa
      901 aatacatcca ttttcttgaa taaatcaaca tgtttattaa tcaattgctg ttttagctct
      961 gactcatcaa gctctccata tttgtcctac agataattgc gaataaatac agagaacgcc
     1021 atttatcctt ttagcatacc tgccggatat ttttatttgc attaatatta attgcatctc
     1081 caactccgag catatccatc attaaaaact ttttgagatt atagaatacc tgtgataaca
     1141 agtgaaacat ctgcacaatg tgaatagtta gtttcttttc agaggaaatg aaatagacaa
     1201 gctgaatgaa ggggagagtg atataaatca aaaaacataa catgaattta attaattaca
     1261 cccatcattg tggataaagt tactgaataa taaataacat aat
//
LOCUS       DAZFWL020000400         1298 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFWL020000400 DAZFWL020000000
VERSION     DAZFWL020000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.406"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..735
                     /locus_tag="ACHKNP_RS27245"
                     /old_locus_tag="pgaptmp_005449"
     CDS             1..735
                     /locus_tag="ACHKNP_RS27245"
                     /old_locus_tag="pgaptmp_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707383.1"
                     /translation="MAPVKGAGTTLWVYTGKGDAYANPLSDDDWQRLAKVKDLTPGEM
                     TAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAY
                     KIRFPNGTVDVFRGWISSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITPATAIKV
                     TPTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMVITVTGVKAGKV
                     SIPVISGNGQFATVAEVTVTEAGAAG"
     gene            749..1180
                     /locus_tag="ACHKNP_RS27250"
                     /old_locus_tag="pgaptmp_005450"
     CDS             749..1180
                     /locus_tag="ACHKNP_RS27250"
                     /old_locus_tag="pgaptmp_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4707384.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTGSPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGPVHVDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            1207..>1298
                     /locus_tag="ACHKNP_RS27255"
                     /old_locus_tag="pgaptmp_005451"
     CDS             1207..>1298
                     /locus_tag="ACHKNP_RS27255"
                     /old_locus_tag="pgaptmp_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4707385.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRMHYF"
BASE COUNT          323 a          296 c          402 g          277 t
ORIGIN      
        1 ctggcgccgg taaaaggtgc cggtaccact ctgtgggttt acaccggcaa gggtgatgct
       61 tatgcaaacc cgttgtcaga tgatgactgg cagcgactgg ctaaggtgaa ggatctgacg
      121 ccgggcgaga tgacggcaga atcctacgat gataactacc tggatgatga agacgcggac
      181 tggagcgcga ccgggcaggg acagaaatct gcaggtgata ccagttttac actggcctgg
      241 aaaccgggag aagaaggtca gaaagggctt ataggctggt ttgaaagcgg ggatgtgcgg
      301 gcctataaaa tccgttttcc gaatggcacg gtggatgtgt ttcgtggctg gatcagcagt
      361 atcggtaagg cagtgacggc gaaagaagtg atcacccgaa cagtgaaagt gaccaacgtg
      421 ggcaaacctt ccgtggcgga agaacgcagc gaaattacgc cggccactgc aattaaggtg
      481 acaccgacat ccggtaccgt ggaaaaagga aaaacaacca ccctgactgt ttcttttgag
      541 ccggaaagtg caaccgacaa gacgttcaga gcggtttccg ccgatccgtc aacgggaacc
      601 attgctgtga aagatatggt gatcactgtg acgggggtta aggctggaaa agtgagtatc
      661 cccgtgattt ccggtaatgg tcagtttgcc acggtagctg aagtcaccgt tactgaagcg
      721 ggcgctgcag ggtaaacgga ggtcatacat gtttctgaaa acagaacaat ttgaatataa
      781 cggtgtgtct gtcacgcttt ccgaattgtc tgcgctgcag cgtattgagc atcttgccct
      841 cctgaaacgg cgtgcagaac aggcagaatc cagcggcaac ctgcaggtaa gcgtggaaga
      901 tctcgtcaga accggcgcgt ttctggtggc gatgtccctg tggcataacc atccgcagaa
      961 aacggggtca ccgtcaatga atgaggccgt gatgcagatt gagcaggaag tcctgaccac
     1021 ctggcctgct gatgccattg cccgggcgga agatgtggtg ttgcgtctgt ccgggatgag
     1081 cgggcctgtt catgtggata cggatattac cgaagtggcg aaaaataacg cgctgactga
     1141 tgatgatttt tctgcgggaa agtcttcgac ggcgagctga attttgccct cagactggcg
     1201 cgtgagatgg ggaggcctga ctggcgcgcc atgcttgccg ggatgacatc caccgaatat
     1261 gccgactggc gacattttta ccgcatgcat tattttca
//
LOCUS       DAZFWL020000401         1288 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFWL020000401 DAZFWL020000000
VERSION     DAZFWL020000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.407"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..953
                     /locus_tag="ACHKNP_RS27260"
                     /old_locus_tag="pgaptmp_005452"
     CDS             <1..953
                     /locus_tag="ACHKNP_RS27260"
                     /old_locus_tag="pgaptmp_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HGU4707386.1"
                     /translation="SVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNEPEQM
                     IENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGAVIPG
                     GSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSGPLSP
                     FRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFALPDES
                     ELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILESQFRL
                     SVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
BASE COUNT          347 a          271 c          324 g          346 t
ORIGIN      
        1 gttcagtggg tattatgtca gatgaagccg gtattatatt tgatggatat accctgtctg
       61 agttgccttt tattaacaaa atgtgggatg gctctgtatt gtctgtggac agaaagaatg
      121 aacccgaaca gatgattgaa aatgccagaa tgacgttatc cctcatggtg caacccggtc
      181 tttttgaccg atatatggag cgaaaaggtt ctgttgccag ggacagtggt tttcttgccc
      241 gttgtcttat cagtaaacct gcaaccacgc aggggaaacg ctttattaac ggagctgtga
      301 ttccgggagg atcattaacg gcatttcatg aacgattaat ggaactggcc agaggcagta
      361 ttgaaaaatc cagtgaggat gaacggtatt gtctgcattt ttcgcctgaa gcgcaaaaaa
      421 tatttattga gcactataat gttctggagc aggatttatc cccttccgga cccttaagcc
      481 cgtttcgggg acatgtttca aaaaaaacag aaaacattgc aagaattgca gcgttatttc
      541 agtatttcag ctatggtgaa gggaaaatct ctgccgatat catgacgtct gctgtcgtaa
      601 taagttcgtg gtacacagat gaatataaaa aattatttgc gttacctgat gaatctgaac
      661 tgcagcagaa ggacgccgag gagctgttcg actggctcat tgaggagtgc cgcggagaat
      721 gcccgccccg tgtcaggaaa aattatattc tccagtgtgg cccgggccgg ttcagaaaca
      781 ggaaaaaact gaacgcattg ctgaacattc ttgaatctca gttcaggctt tctgtcgtgc
      841 cggaaggaaa aaccatgtat gttctgcttc cccagatagc cagtttaaag ttgtctgatg
      901 tctctggtat ttttacctcg ggttatcact acaataagct gagagcgaag taaccggtga
      961 gcagtttttc tccattatgc ttcagactta gatgattgat tattatgggg atttcatcac
     1021 cgatattcag atgtatgaat atcggtgatg aactaaagct caggagctgc gtggtggtga
     1081 taaatcaaca ggcatataac tgatacccgc cggtcagaag ttccagcctg cgactttcct
     1141 gtctgcgtgc ccgcagtccg gcgttcaggt gcagcgtgtg acagaacgca cggagagcga
     1201 aacggcaggc agacaggccg ttcagtcagc cctggcaccc tgcccggata ccagcttccc
     1261 tgaaacaact ggcatcaggt gcgcttat
//
LOCUS       DAZFWL020000402         1287 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFWL020000402 DAZFWL020000000
VERSION     DAZFWL020000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.408"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            135..437
                     /locus_tag="ACHKNP_RS27265"
                     /old_locus_tag="pgaptmp_005453"
     CDS             135..437
                     /locus_tag="ACHKNP_RS27265"
                     /old_locus_tag="pgaptmp_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HGU4707387.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            440..799
                     /locus_tag="ACHKNP_RS27270"
                     /old_locus_tag="pgaptmp_005454"
     CDS             440..799
                     /locus_tag="ACHKNP_RS27270"
                     /old_locus_tag="pgaptmp_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4707388.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(846..1238)
                     /locus_tag="ACHKNP_RS27275"
                     /old_locus_tag="pgaptmp_005455"
     CDS             complement(846..1238)
                     /locus_tag="ACHKNP_RS27275"
                     /old_locus_tag="pgaptmp_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4707389.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
BASE COUNT          365 a          259 c          290 g          373 t
ORIGIN      
        1 ccctgcgttc tgtggggcgg ggtgggttta tggatacaat ttacaaatta aaatttaact
       61 ggtcaacatg tttattatta aatttgtaat tgcgggctgt tgcatcagtc ccaaaatggg
      121 actactatac agttatgaag attatctcag ttaaaacact cagggatttt tgggcggaga
      181 atcctgatgc agaacaaccg ctaagggcat gggtggatga ggcggcaaaa gctgactgga
      241 aaagcccagc agacattaag gcacaatacc gaacggccag tatattgaaa aaccggcgcg
      301 tggtattcaa cataaaaggt aatcattacc gtttaattgt tgcaattgcg tatcagcgag
      361 gatgggtatt tgttaaattc atcggaaacc acaaggaata cgatgctatt gatgctgata
      421 ccatcgaact ggagtaagca tgaacatcaa acctattcgt acagagcaag attatgaagc
      481 cgcgttgcgt gctgttgaac ccatgttcga caatgagccc gaaattgaca cgcctgaggg
      541 ggattttttt gaggtgatgt gtttgctaat agaggaatat gaaaaaaaac attatcccat
      601 tgagccacca tccccaattg aagctataag atttcgcatg gaacagcagg ggctgactgt
      661 gaaagatttg gaacccgcaa ttgggaaaaa aaatcgggtt tatgaggtgt tgaatggtac
      721 cagaagcctt acgttaccaa tgattcgccg tcttcataat caatttggta tccccctgga
      781 aagcctggtt ggattataaa atctgctagt catttgcctg atgctcgttc cagaaaagga
      841 atgcatcagg cagttttgtt tttctgccgc agtaactctt caagtttccg tttatagaaa
      901 tcgcgttttt gctccatatc acgaatgatc tgctctgcgt cgctttgagg taactcatct
      961 aaaagcgata tgatttttcg ttgttgttct gtaagttgcg gttggttgtc attactggat
     1021 acagccattt tatcgccgag agtttcttct tccataaaga accaatggac gggatgttgt
     1081 gagagctctg ctaatttttc cagtttatcc attcttggca tcacgccttt caaccaacct
     1141 tgcacggatt ggggttttac accaagacgt cttcccagct ctgactggtt tatattcaat
     1201 tcctgcaaca cctgctgaag gcgttttaca aagatcatca ccacccctcg taaactagtt
     1261 ccgcgatcct acagaaaaaa ttgataa
//
LOCUS       DAZFWL020000403         1265 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFWL020000403 DAZFWL020000000
VERSION     DAZFWL020000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.409"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..643)
                     /locus_tag="ACHKNP_RS27280"
                     /old_locus_tag="pgaptmp_005456"
     CDS             complement(<1..643)
                     /locus_tag="ACHKNP_RS27280"
                     /old_locus_tag="pgaptmp_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4707390.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMVEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKF"
     gene            complement(643..972)
                     /locus_tag="ACHKNP_RS27285"
                     /old_locus_tag="pgaptmp_005457"
     CDS             complement(643..972)
                     /locus_tag="ACHKNP_RS27285"
                     /old_locus_tag="pgaptmp_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707391.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(969..>1265)
                     /locus_tag="ACHKNP_RS27290"
                     /old_locus_tag="pgaptmp_005458"
     CDS             complement(969..>1265)
                     /locus_tag="ACHKNP_RS27290"
                     /old_locus_tag="pgaptmp_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4707392.1"
                     /translation="FVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSLADSRSQASG
                     TFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
BASE COUNT          266 a          420 c          303 g          276 t
ORIGIN      
        1 ggaacttaca accgctcagg catttgctgc atttatcctt cgtgatatcg gacgttggct
       61 ggtcatattc atccgcgaca gccggaccgc tataaccgca ctcgtcaccg cgataggtcc
      121 aggtgcaggt gttggccagc atgatacgtc ccggaaaaac agcgccatcc gtttccgtcg
      181 gcgtggacag tacaaaggag gcactcaccg cgctcagttc gctgcactgc tcaatgcgcc
      241 agcggctgat cacctcctgc tccggatcgg cgtaactgtt tccgttgacg aagttcaccg
      301 catccagaaa acgggcgtaa accttacgcc tgaccaccgt tccgccgacc agactctgca
      361 tatcttccac cataccggtg accatgccgt gcaggttaga aaccgtcagt gtcggacggg
      421 cagcactgcc cttgccgttc agttcaaatc ccgtcccctg aatgggatac gcctgatact
      481 gtcgcccctg ccaggtgacc ggctcacctt tttcgttctg ctcattacag aaaaaataac
      541 gttctccacc gacctctgtc aggtcgattt cccagagcac cacgctggcc gactgctccg
      601 cacgggtgca ttcattcagt gtttcctgcc ggatatcctg catcagttca ccacctgttc
      661 aaactctgcg ctgaactcaa cacgcagcat actgacccgc gacgaccatt ttgcgcaggt
      721 cacctttatc tgccgccact cataaggcgg cgtccacaga aaggatttcc agcccccgtg
      781 ctcttccaga aacgactcca gtaccgtggc ctcctcacgg gggacagaaa gcgtcacgct
      841 gtacgttttc aggttggcat tcagcccggc aggcgctcgc tgggaatagc catcaccaaa
      901 gcgcaccttt cttacagaag gggccgaagc cacatccata ccgggtttca ctttccagcg
      961 gaaggttttc atcgtccacc tccggagaac aggccaccat cacgcatctg tgtctgaatt
     1021 tcatcacggg cacccttgcg ggccatgtca tacaccgcct tcagagcagc cggacctatc
     1081 tgcccgttcg tgccgtcgtt gttaatcacc acatggttat tctgctcaaa cgtcccggac
     1141 gcctgcgacc ggctgtcagc cagactgccc ggtgtaccga cataaccacc ggtggcatag
     1201 ccgcgcatca gccggtagag attccccacg ccaatccggc tggttgcctc cttcgtgaag
     1261 acaaa
//
LOCUS       DAZFWL020000404         1265 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFWL020000404 DAZFWL020000000
VERSION     DAZFWL020000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.410"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..643)
                     /locus_tag="ACHKNP_RS27295"
                     /old_locus_tag="pgaptmp_005459"
     CDS             complement(<1..643)
                     /locus_tag="ACHKNP_RS27295"
                     /old_locus_tag="pgaptmp_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4707393.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKF"
     gene            complement(643..972)
                     /locus_tag="ACHKNP_RS27300"
                     /old_locus_tag="pgaptmp_005460"
     CDS             complement(643..972)
                     /locus_tag="ACHKNP_RS27300"
                     /old_locus_tag="pgaptmp_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707394.1"
                     /translation="MKTFRWKVKPGMDVTSAPSVREVRFGDGYSQRVPAGLNADLKTY
                     SVTLSVSREEATALESFLAEHGGWKAFLWTPPYGYRQIKVTCAKWSSQVSMLRVEFSA
                     EFKQVVN"
     gene            complement(969..>1265)
                     /locus_tag="ACHKNP_RS27305"
                     /old_locus_tag="pgaptmp_005461"
     CDS             complement(969..>1265)
                     /locus_tag="ACHKNP_RS27305"
                     /old_locus_tag="pgaptmp_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4707395.1"
                     /translation="FVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASG
                     TFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
BASE COUNT          265 a          421 c          307 g          272 t
ORIGIN      
        1 ggaacttaca accgctcagg catttgctgc atttatcctt cgtgatatcg gacgttggct
       61 ggtcatattc atccgcgaca gccggaccgc tataaccgca ctcgtcaccg cgataggtcc
      121 aggtgcaggt gttggccagc atgatacgtc ccggaaaaac agcgccatcc gtttccgtcg
      181 gcgtggacag tacaaaggag gcactcaccg cgctcagttc gctgcactgc tcgatgcgcc
      241 agcggctgat cacctcctgc tccggatcgg cgtcactgtt tccgttgacg aagttcaccg
      301 catccagaaa acgggcgtaa accttacgcc ggaccaccgt tccgccgacc agactctgca
      361 gatcttccgc catcccggtg accataccgt acaggttaga aaccgtcagc gtggggcgcg
      421 tactggtgcc tttgccattc agttcaaaac cgctcccctg aatgggatac ggctgatact
      481 gtcgcccctg ccaggtgacc ggctcacctt tttcgttctg ctcattacag aaaaaataac
      541 gttcaccacc gacctctgtc agatcgattt cccagagcac cacgctggcc gactgctccg
      601 cacgggtgca ttcattcagt gtttcctgcc ggatatcctg catcagttca ccacctgttt
      661 aaactctgcg ctgaactcaa cacgcaacat actgacctgc gacgaccatt ttgcgcaggt
      721 cacctttatc tgcctgtaac cataaggcgg cgtccacaga aaggccttcc agcccccgtg
      781 ctcagccaga aacgactcca gcgccgtggc ctcctcacgg gagacagaca gcgtcacgct
      841 gtacgttttc aggtcagcgt tcagcccggc aggcacacgc tgggaatagc catcaccaaa
      901 gcgcacctcc ctgacggaag gggccgatgt cacatccata ccgggtttca ctttccagcg
      961 gaaggttttc atcgtccacc tccggagaac aggccaccat cacgcatctg tgtctgaatt
     1021 tcatcacggg cacccttgcg ggccatgtca tacaccgcct tcagagcagc cggacctatc
     1081 tgcccgttcg tgccgtcgtt gttaatcacc acatggttat tctgctcaaa cgtcccggac
     1141 gcctgcgacc ggctgtctgc catgctgccc ggtgtaccga cataaccgcc ggtggcatag
     1201 ccgcgcatca gccggtaaag attccccacg ccaatccggc tggttgcctc cttcgtgaag
     1261 acaaa
//
LOCUS       DAZFWL020000405         1260 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFWL020000405 DAZFWL020000000
VERSION     DAZFWL020000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.411"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1145
                     /locus_tag="ACHKNP_RS27310"
                     /old_locus_tag="pgaptmp_005462"
     CDS             <1..1145
                     /locus_tag="ACHKNP_RS27310"
                     /old_locus_tag="pgaptmp_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4707396.1"
                     /translation="SVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLA
                     LKETEVAKTSLNTYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYI
                     VTQPGRSAVTVNNYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFI
                     RLIDACRNQQAKNLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPK
                     TQAGTNRVIHLIKPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVS
                     ARVKNYGDHFTVDSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQM
                     GHADAQMVFQVYGKWMSENNNAQVALLNTQLSKFAPTMPHNEAMKN"
BASE COUNT          413 a          267 c          262 g          318 t
ORIGIN      
        1 cctctgtttg ttacgcaata aaaactggtg ttttcgacta tgcaaaacag tttccctcct
       61 cacgcaatct ggaaaaattt ggtgaggccc gacaagattt aaccataaaa gaactggctg
      121 aaaaattttt ggcactgaaa gaaactgaag tcgccaaaac atcactcaac acataccgtg
      181 ccgtcatcaa aaatatcctg agcataatcg gtgaaaaaaa tcttgcctca tcgattaata
      241 aagaaaaatt actggaggtt cgtaaagagc tactgactgg ataccagatc cccaaaagta
      301 actatattgt tacacaacca gggagatcgg ctgtaactgt aaataattac atgacaaatc
      361 ttaacgccgt gttccagttt ggtgttgata acggttacct ggcagataat ccgtttaagg
      421 ggatctcgcc attaaaggaa tcaagaacca ttccggatcc tctttcgcgg gaggaattta
      481 tccgtcttat cgatgcgtgc agaaatcagc aagcaaaaaa tttatggtgt gtttctgttt
      541 atactggcgt tcgccctggt gagctgtgtg cacttggatg ggaggacata gatctgaaaa
      601 atggaacaat gatgatcagg agaaatttag caaaagaccg tttcacggta ccaaaaacac
      661 aggcgggaac caatcgggtc attcatctta ttaagccagc aatcgacgct ctccggagtc
      721 agatgacatt aacgagactg agcaaagagc atatcattga tgttcacctc agagagtatg
      781 gcagaacaga aaaacaaaaa tgcacctttg tttttcaacc tgaagtgtca gcgagagtaa
      841 aaaattatgg tgaccatttt accgttgact caataaggca gatgtgggac gcagcgataa
      901 aacgtgccgg actccgccat cgaaaatcat atcagtcgag acatacttat gcctgctggt
      961 cgctgacagc tggtgctaac ccggcattta tagcaaacca gatgggccat gcagatgcgc
     1021 aaatggtatt tcaggtatac ggaaaatgga tgtctgaaaa caataatgca caggtagctt
     1081 tgttaaatac acagttaagc aagtttgccc caaccatgcc ccataacgaa gcaatgaaaa
     1141 attaatttaa tatttatcaa atagttaaca cgcatgactc ttgaaatcca taaattcaag
     1201 cgcagtgccc agccatcccg atactgctgc tttcaccaaa tccttagtgc ttctttcatg
//
LOCUS       DAZFWL020000406         1246 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFWL020000406 DAZFWL020000000
VERSION     DAZFWL020000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.412"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..90
                     /locus_tag="ACHKNP_RS27315"
                     /old_locus_tag="pgaptmp_005463"
     CDS             <1..90
                     /locus_tag="ACHKNP_RS27315"
                     /old_locus_tag="pgaptmp_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707397.1"
                     /translation="YFVIRGNVEQIEPNLLGVYAGLLLAMKNK"
     gene            complement(126..>1246)
                     /locus_tag="ACHKNP_RS27320"
                     /old_locus_tag="pgaptmp_005464"
     CDS             complement(126..>1246)
                     /locus_tag="ACHKNP_RS27320"
                     /old_locus_tag="pgaptmp_005464"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HGU4707398.1"
                     /translation="VTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIKNPGYSNYT
                     FVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNSMK
                     LYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPN
                     GIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLK
                     LVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVIPS
                     QFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTL
                     ANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          388 a          248 c          212 g          398 t
ORIGIN      
        1 tattttgtta tccgtggaaa cgtagaacaa attgaaccaa atttattagg cgtttacgcc
       61 ggcttattat tagcgatgaa aaacaagtaa aaaataaaaa aggctgcata atgcggcctt
      121 tttatttatt caaaccaatt atgaataacc tcttcgaatc tttgagttac gccatcccaa
      181 ctgtagtgtt caaacacaaa gtcctgacct tgctttgcaa ctgctgttaa ttcaggattc
      241 gctaacgttt ttaaaatatc gctgcttatg gagtctgcag tcataggttc ttttaaatgg
      301 aatcccgtag tattttcttt gacaaattcc gtcatccccc cacgcgtact cactaaaaca
      361 ggcttaccag cacccatagc ttcaattgcc accatgcaaa atggttcctg gaactgggaa
      421 ggaatgacga ctaaatctgc cagtgggtaa taacaatgca tcttttcagg cggtatgctt
      481 cctaacataa tacaacgatc cttcaaacgt tttgcaattt ccctgacatt gcgttgatat
      541 gcacctttgt cacttttact catttctgta taatcaccaa tcacaactaa ttttaaattt
      601 ttgtgagcag cggccaattt ttcgaatgat tgcatcaaca gcaatatgcc tttgtcaggg
      661 acgatacgcc cagcataaaa aataattttc tcttcagggg agatattaag ttcgcttcta
      721 gtgatcggct gaaaattaga ttgataggtt tctaaatcaa ttccatttgg tacaatctca
      781 atatctgcat ttgctaaata agattggtaa tactttttca agtataaact aggcactatc
      841 attttaacat tttgctcaag caattttggt tcaaatgcat tatgcatatg tataaccact
      901 cttgcctgag gagctcgctt acgaatttga gtgtatagct tcatgctatt atgaactaca
      961 ataacacttt ctttcgttat aggaaaatca tgtgcaatat tcaatattct ctgtgaatat
     1021 ggaagaggat caagtcttgt ccatttttga aataaccgct tatatatgcg actgaatcca
     1081 accctatgaa tactacaatt atcattaaca aaggtgtaat ttgagtatcc tggatttttg
     1141 atacaaacaa tacgatttgg aaaattggta cgctgagcaa cctgatacat ccacgtttca
     1201 acagcagccg ctccgcgagg aggaattgaa aaaataggag taaccg
//
LOCUS       DAZFWL020000407         1236 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFWL020000407 DAZFWL020000000
VERSION     DAZFWL020000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.413"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(45..371)
                     /gene="ybfB"
                     /locus_tag="ACHKNP_RS27325"
                     /old_locus_tag="pgaptmp_005465"
     CDS             complement(45..371)
                     /gene="ybfB"
                     /locus_tag="ACHKNP_RS27325"
                     /old_locus_tag="pgaptmp_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HGU4707399.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(371..>1236)
                     /locus_tag="ACHKNP_RS27330"
                     /old_locus_tag="pgaptmp_005466"
     CDS             complement(371..>1236)
                     /locus_tag="ACHKNP_RS27330"
                     /old_locus_tag="pgaptmp_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4707400.1"
                     /translation="VSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLP
                     LALISTEGATAWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDP
                     LQGRYITQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMGGTGERSGPDI
                     WGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVETD
                     NSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETCKMKCSGNNIG
                     RFIRFVFTGVM"
BASE COUNT          347 a          311 c          225 g          353 t
ORIGIN      
        1 tcaatgccca ggttacatat aataataaga gcaattaact taatttattt gtttataatg
       61 cgcttactta atacaaacca ttgaatatac cctcctgaca gaaaataaag ccatattatt
      121 atcgccataa tatacttact atcaatcctt acgccaataa tgtctgatac agtccctata
      181 aatattagag caataaagaa tactatgcct gttggaaaac atattaccat cattccataa
      241 gacatgagac taattaactc gctcacatca cgagtggagt ctaatagtga aagtctatgc
      301 atggaaaaga atagtattaa aagcgataaa gccagccata tagctctgaa taaaaaaata
      361 atatatttca ttacatcact ccggtgaata caaatctaat aaaacgtcca atgttatttc
      421 cggagcactt catcttacat gtttctaaag aatcattaga ccagtcttgg cagtttttaa
      481 atagccctaa atttataggt gtgaatatat caggcaatac agaataacgc ggccttacct
      541 ctttaaactt cccttttaaa cagttctcca cacaactatt atcggtttca acgaggtcac
      601 aacggccagc agcttctttt gtatcattac ttgctttacc tggcccccat agaccatctc
      661 cttttgattc tccccgttgg tcttggccac cacaagtata gtccccttta ccatctggga
      721 cacaaagata ttgatgatag aacggattcc cccatatatc tggaccgctt ctttcaccag
      781 ttccgcccat tgaatgcagt ggcttaatgc attttagtgt ttctaatcct aatggatcta
      841 tatttgagat cggattcaac ggatactgat aaaaattcca tccccccttc agcccaatcg
      901 gatcctgagt gatataccgc ccctgcagcg ggtcataata gcggtggcgg ttgtaataca
      961 ggccggactc ctcatcatac tgctgccccg gcagccggat aagctgctgc agctgatgcg
     1021 ggttctcttc attcagcagg ttgccccatt catcatattc tgcgcaccac gctgttgccc
     1081 cttccgtgct gatgagcgcc agcggcaggc cgcgatggtc gcagtggtac aggtggattt
     1141 ttcgcgccgg cgtgtacacc gggtccatct ggttttgcat ctgctccacg gtcaggccgc
     1201 acgatgccag ccagcggcgg ctttcctcac tcaccc
//
LOCUS       DAZFWL020000408         1233 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFWL020000408 DAZFWL020000000
VERSION     DAZFWL020000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.414"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..198)
                     /locus_tag="ACHKNP_RS27335"
                     /old_locus_tag="pgaptmp_005467"
     CDS             complement(<1..198)
                     /locus_tag="ACHKNP_RS27335"
                     /old_locus_tag="pgaptmp_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HGU4707401.1"
                     /translation="MNEQFRTDLAHTFGITLEEQTNALSFHDHDGHEWILECAPQSEV
                     LFFYCYLFNNESSKIDKILEIN"
     gene            449..1060
                     /locus_tag="ACHKNP_RS27340"
                     /old_locus_tag="pgaptmp_005468"
     CDS             449..1060
                     /locus_tag="ACHKNP_RS27340"
                     /old_locus_tag="pgaptmp_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HGU4707402.1"
                     /translation="MFSPMTMVGRSLAQAATQTLRPAVTRAATQAGVGASGMKFVPMQ
                     SNFMINHGKLTNQLLQAVAKQTRSGDTQQWFKQEQITYISRTVNRAIDDYCMSNNSVI
                     GKETKSHIFKAVESAIKQPLDMNCAQTSIGHFLQSNKYFNKKVDEQCGKGVDPITRFN
                     TQTRMIEQVSQEIFEQKFGGLKVSDIKALTQSTIAQHVQDTRL"
BASE COUNT          411 a          199 c          253 g          370 t
ORIGIN      
        1 atttatttct agtattttat caatttttga agattcatta ttgaataaat aacaataaaa
       61 aaacaggacc tcagattgag gtgcacactc cagtatccat tcatgaccat cgtgatcatg
      121 gaaagatagg gcgttagttt gttcttcaag agttattccg aacgtatgtg caagatctgt
      181 gcgaaattgt tcattcatat tgtagtcctg tcattctttc aatgagggta tgcttaaacg
      241 taattaactc gtgagctctg gcacaaataa taagacgaaa atataagatg caagtaatag
      301 tgttgcatat ttatgatagc atgtgataaa tgtagtgcaa ctacttgtag atagtgccgg
      361 aggaaaattt aaatataaaa caattattgt ttttaatgaa aataaaaaac attttgttat
      421 cgttatataa tatataaagg tttattgtat gtttagtcca atgacaatgg taggtagatc
      481 gttagctcag gcggctacac aaactcttag acctgcggtc accagagctg cgacacaagc
      541 gggggtggga gccagtggca tgaagttcgt gcccatgcag tcgaacttta tgattaatca
      601 tggcaaactg actaaccagc tacttcaggc cgtggccaaa caaacccgta gcggtgatac
      661 tcagcaatgg ttcaaacagg agcaaattac ttatatatcc agaacagtaa acagagctat
      721 tgatgactat tgcatgagta ataattcggt aattggtaag gaaaccaaaa gccacatttt
      781 caaggctgtt gaaagcgcga taaagcagcc tctcgatatg aattgtgcac aaacgtcgat
      841 tggccatttc ttacagtcca acaaatactt taataaaaaa gttgatgagc agtgtggcaa
      901 aggggtggat ccaattacac gctttaacac ccagaccagg atgatagaac aagtctcaca
      961 agaaatcttt gaacagaaat ttggtggttt gaaagttagt gatatcaaag ctctgactca
     1021 gagcacgatt gcacagcatg tacaggatac tcggttgtag gaataacctg ttatatatgc
     1081 gtgttgggca taagttaagc ttcctggtgt atagcgtagt ctttgctgat gtaagaaaag
     1141 gctatatacg gaaacttgat atactataaa cttcattatt tattatattg aaaaagttat
     1201 ttaattgatt tgttttgtaa tttcaatgct tca
//
LOCUS       DAZFWL020000409         1220 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFWL020000409 DAZFWL020000000
VERSION     DAZFWL020000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.415"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            523..1220
                     /locus_tag="ACHKNP_RS27345"
                     /old_locus_tag="pgaptmp_005469"
                     /pseudo
     CDS             523..1220
                     /locus_tag="ACHKNP_RS27345"
                     /old_locus_tag="pgaptmp_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545887.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          358 a          224 c          245 g          393 t
ORIGIN      
        1 tttggtcgat gacttcttcc ggcgaatact gatagaaaca ttgaaatatt gtaagagaaa
       61 gcgttcaaaa ttctgtggca cgtgaaaaat tctacagcgt catcacctta ttcagtcgac
      121 cttcaaaata aacgtttaac agagacaatc accatttgga ttgtccgctg aggaggcgaa
      181 caatttccta gacagcttta tcatgtcagc ccacattgtt ggcgctggca atactaagga
      241 caaacgatgg ggaagtagct ttttagcgtt attttcggga gtaaaaaacg tggtatcttt
      301 ccgtatgtca gttgtatctt gctggtattc gtagtcgtga taaggcatac aacgatattt
      361 atcttgacgt tagtgaagaa taagttgttt tgtgatctat gttaatctgg gattacaatc
      421 cgggcttgat acatcgcatc acgactttta ttctcttcag aatagaatat gttactcccc
      481 gctttacagg ctcaggattt gaattacaaa ttatactttt aagtgaacaa taaccaaggg
      541 tatttcttat ttcctgaaac caggcttggc ccatttcggg atgagtttga aaaaactctt
      601 gacgcatacg atagtgatga aataaacgaa acaaattacg tcaacaaact caggcgtctt
      661 gcctgacatg ctcctgacat cattgatatt cacgctcatc tggcatatgc atttgcgctc
      721 tttatgctgc aattctggcg aatgttcatt tacagcgtca tcaggatgcc gttatactca
      781 ttgataaaat actaacatat aaccctgaga ataatcaggg aacacgctgg ttactggggg
      841 cggaattgtt acgtgctggc aagcatggca gggctctgta cgtattgaca ggacatgtca
      901 atgaatttcc cccctactgg tacgagcttg ctcttttaca ttttatgaat gaggcatata
      961 ttaatgctgc aacggcattt cgatatggat ttttaacaag tatatattgt tgaaatgctt
     1021 tgttagaatc tttatccatt ccctagggct gtatagtatg atttttcagg tatttctgaa
     1081 tactgccaaa tattattgca aaatattctc taaattatga attaaatatc cataagcatt
     1141 attgcctggg aactggacat ataattactc ttctgtgcta aaggaacgtt ctgaaatgtt
     1201 taagtatgca gagatgttga
//
LOCUS       DAZFWL020000410         1210 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFWL020000410 DAZFWL020000000
VERSION     DAZFWL020000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.416"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..692
                     /gene="nleB"
                     /locus_tag="ACHKNP_RS27350"
                     /old_locus_tag="pgaptmp_005470"
     CDS             <1..692
                     /gene="nleB"
                     /locus_tag="ACHKNP_RS27350"
                     /old_locus_tag="pgaptmp_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HGU4707403.1"
                     /translation="KKEFLSLENEYQNIKIIYRADVDFSMYDKKLSDIYLENIHKQES
                     YPASERDNYLLGLLREELKNIPEGKDSLIESYAEKREHTWFDFFRNLAMLKAGTLFTE
                     TGKTGCHNISPCSGCIYLDADMIITDKLGVLYAPDGIAVHVDCNDEIKSLENGAIVVN
                     RSNHPALLAGLDIMKSKVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCDFIEFKHENII
                     PNTSMYTSSSW"
     gene            741..>1210
                     /gene="nleE"
                     /locus_tag="ACHKNP_RS27355"
                     /old_locus_tag="pgaptmp_005471"
     CDS             741..>1210
                     /gene="nleE"
                     /locus_tag="ACHKNP_RS27355"
                     /old_locus_tag="pgaptmp_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HGU4707404.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPLG"
BASE COUNT          410 a          186 c          243 g          371 t
ORIGIN      
        1 aaaaaaagga atttctcagt cttgaaaatg aatatcaaaa tataaaaata atctaccgag
       61 cagatgtgga tttcagcatg tatgataaaa aactatctga tatttacctt gaaaatatcc
      121 ataaacaaga atcataccct gccagtgaga gggataatta tctgttaggc ttattaagag
      181 aagagttaaa aaatatccca gaaggtaagg actctttgat tgagtcatat gcagaaaaaa
      241 gagaacatac ttggtttgat tttttcagga atttggccat gttgaaggct ggaactttgt
      301 ttacagagac gggaaaaact ggatgccata acatatcgcc ctgtagcgga tgtatatatc
      361 ttgatgccga catgattatt accgataaat taggagtcct gtatgctcct gatggtatcg
      421 ctgtgcatgt agattgtaat gatgagataa aaagtcttga aaatggtgcg atagttgtca
      481 atcgtagtaa tcatccagca ttacttgcag gcctcgatat tatgaagagt aaagttgacg
      541 ctcatccata ttatgatggt ctaggaaagg gtatcaagcg gcattttaac tattcatcgt
      601 tacacgatta taatgctttt tgtgatttta ttgaatttaa gcatgaaaat attataccga
      661 ataccagtat gtataccagc agttcatggt aagtcttccg gttgtggttg aaaaatatcg
      721 ttctttaggt atagattcag atgattaatc ctgttactaa tactcagggc gtgtccccta
      781 taaatactaa atatgctgaa catgtggtga aaaatattta cccggaaatt aaacatgatt
      841 actttaatga atcacccaat atatatgata agaagtatat atccggtata accagaggag
      901 tagctgaact aaaacaggaa gaatttgtta acgagaaagc cagacggttt tcttatatga
      961 agactatgta ttctgtatgt ccagaagcgt ttgaacctat ttccagaaat gaagccagta
     1021 caccggaagg aagctggcta acagttatat ccggaaaacg cccaatgggg cagttttctg
     1081 tagatagttt atacaatcct gatttacatg cattatgtga gcttccggac atttgttgta
     1141 agatcttccc taaagaaaat aatgattttt tatacatagt tgttgtgtac agaaatgaca
     1201 gccctctagg
//
LOCUS       DAZFWL020000411         1189 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFWL020000411 DAZFWL020000000
VERSION     DAZFWL020000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.417"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(163..1038)
                     /locus_tag="ACHKNP_RS27360"
                     /old_locus_tag="pgaptmp_005472"
     CDS             complement(163..1038)
                     /locus_tag="ACHKNP_RS27360"
                     /old_locus_tag="pgaptmp_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HGU4707405.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTPGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
BASE COUNT          320 a          241 c          233 g          395 t
ORIGIN      
        1 ttgatattta ctgagcgctc agatcaactt tccatggcaa cagatcgcgt acccggtttg
       61 ccggccagtc ctggatatgc tcccgccgac aatattaatg gccaggaaat gtttaaaaca
      121 atgattctgt ttcctaacca tcaagtgtat atgctggctc aattactcaa tatctatggg
      181 ttgagttcta cccaaagtgg ctctgtagtc gcttcttctt acagtatgtt caggtgttgc
      241 aggcacagtt gtggagcgta gatgcgttgt tggtttacca tgctctggta cataaaaaac
      301 gccagtgcca tggtaacctg atatagaaac gttaattccg cgacgttcaa actcggcata
      361 gacgtgttct gctaaagacc tcttttgacc aaataatgcc ctggccaacc agccgttatt
      421 catatttgcg gatttttcta tttcatcagg agagaagtct ttgtttttta ttatgttggc
      481 tgagttacag gatgttaatc tgatatcatc tatttcatga agattatgtt gaacaatgcg
      541 gtcgacaata tctgacggtg ataaaaattc atctccgcac ttaagaagag gtagaccagc
      601 agaaccatgc ccagacagat aaattttgtt gtattttcct ggtgttaaat catctggcag
      661 gatggttttc atctctgtcg ctgtaatact tatagctgca gcagcaacaa catcgctatt
      721 acttgattgt agatggcttt tatttgctcc aggatatgta aactccattt tccttttatc
      781 aaaatcttgt tttgtagcat cattcaggaa taataaatca tacggttttt tccccgttgt
      841 gactcttgaa acattttggc caagaatatt aagagtatag tcattaacgg atttcattcc
      901 taaaaaaaca agtaatcctt ctttctcttg cgctttttcg attgttctcc ctgctaaagt
      961 tattgggatg gattgagatt gttttgtcgg aactgatacg ctggcgctaa taggtagtag
     1021 ggttcttatg ctaaacatac aacctctttc ttaattttcg aacctgttta ggattctgtg
     1081 taaattcaaa ataaacctgt ctgaacattc ctaacaaaca tccaccggac atgacaacaa
     1141 aaaccggagc cggactccgg tttttgtgaa gctgtcggct atttcattc
//
LOCUS       DAZFWL020000412         1181 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFWL020000412 DAZFWL020000000
VERSION     DAZFWL020000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.418"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1181
                     /locus_tag="ACHKNP_RS27365"
                     /old_locus_tag="pgaptmp_005473"
     CDS             <1..>1181
                     /locus_tag="ACHKNP_RS27365"
                     /old_locus_tag="pgaptmp_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU4707406.1"
                     /translation="PRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTAL
                     VGVQVDLEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWC
                     LWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQR
                     KAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSA
                     LKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGL
                     WLIKTELLETQTVDFSVGAEGLRHVPGDVIEIFDDDYAGISTGGRVLAVNSQTRTLTL
                     DREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWGLKLPT
                     LR"
BASE COUNT          263 a          310 c          376 g          232 t
ORIGIN      
        1 ccgcgcccgt ttaatatccg gatgcgcagg atgacgccgg acagcaccac agaccagctg
       61 cagaacaaaa cgctctggtc gtcatacacc gaaatcatcg atgtgaaaca gtgctacccg
      121 aacacggcac tggtcggcgt acaggtggac ttggagcagt tcggcagcca gcaggtgagt
      181 cgtaattatc atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg
      241 cgacaataca gcggtatctg ggacggaacg tttaaaccgg catacagcaa caacatggcc
      301 tggtgtctgt gggatatgct gacccatcca cgctacggca tggggaaacg tcttggtgcg
      361 gcggatgtgg acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtaccg
      421 gacggctttg gcggcacgga gccgcgcatc acctgtaatg catacctgac cacacagcgc
      481 aaggcgtggg atgtgctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac
      541 gggcagacgc tgacgttcgt gcaggaccga ccatcagata aggtgtggac ctataaccgc
      601 agtaatgtgg tgatgccgga tgatggcgcg ccgttccgct acagcttcag cgccctgaag
      661 gaccgccata atgccgttga ggtgaactgg attgacccga acaacggctg ggagacggcg
      721 acagagcttg ttgaagatac gcaggccatt gcccgttacg gtcgtaatgt cacgaagatg
      781 gatgcctttg gctgtaccag ccgggggcag gcacaccgcg ccgggctgtg gctgattaaa
      841 acggaactgc tggagacgca gaccgtggat ttcagcgtgg gtgctgaagg gcttcgccat
      901 gtaccgggcg atgttattga aatcttcgat gatgactatg ccggtatcag caccggtggt
      961 cgcgtgctgg cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg
     1021 ccatcctccg gtaccacgct gataagcctg gttgacggaa gtggtaatcc ggtcagcgtg
     1081 gaggttcagt ccgtcaccga cggacttaag gtgaaagtga accgggttcc tgacggcgtt
     1141 gcagaataca gtgtgtgggg gctgaagctg ccgacgttgc g
//
LOCUS       DAZFWL020000413         1171 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFWL020000413 DAZFWL020000000
VERSION     DAZFWL020000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.419"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(166..813)
                     /locus_tag="ACHKNP_RS27370"
                     /old_locus_tag="pgaptmp_005474"
     CDS             complement(166..813)
                     /locus_tag="ACHKNP_RS27370"
                     /old_locus_tag="pgaptmp_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU4707407.1"
                     /translation="MPINLTPYLTASGGLGEIPQDTLSGIRTLAFNGGAQVQMGSTIV
                     TIRSVFLGFFMGSVSPEGLSERALRTALNNVNRLERDLNGGLSGRQILASIPSPYASP
                     PRPSVMQTELVLEQIEKCSFNVNSSSLRATEEALTCPITLCIPEHGVIMRNAGDSDVC
                     TLYDKESLKHLVNTSSPHPLSREKITESMIVKENSCYFDFTSGSIKNVRGEITRL"
     gene            complement(1023..>1171)
                     /locus_tag="ACHKNP_RS27375"
                     /old_locus_tag="pgaptmp_005475"
     CDS             complement(1023..>1171)
                     /locus_tag="ACHKNP_RS27375"
                     /old_locus_tag="pgaptmp_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4707408.1"
                     /translation="ENGIIPEQARVITQVVILDADKKQIQCVVRPLQILRADGTWENI
                     GWMK"
BASE COUNT          346 a          279 c          187 g          359 t
ORIGIN      
        1 tctgttatgc tttttttcac gcacatttcc aattatgcta acagaaatgt ccagacaata
       61 ccctctcctt ttatccctgt atacagtcca tcaattagaa aattaacaga aaaaactgaa
      121 aagatgtctg tgaattacac ttatcagtca atatgttcta acatcttata gtctagttat
      181 ctcaccacga acatttttaa tacttcctga tgtgaaatca aaatagcacg agttttcctt
      241 aacaatcatt gactctgtta ttttttctct gctcagagga tgaggtgaac ttgtgttaac
      301 aagatgtttt aatgattctt tatcatataa agtacataca tctgaatctc cagcatttct
      361 cataatcaca ccatgctcag ggatgcataa tgtaatcggg catgttaatg cctcttcagt
      421 cgccctcaga gatgacgagt taacattaaa tgagcatttt tctatttgct ctaacaccaa
      481 ctctgtttgc attacagaag gacgaggtgg agaagcatac gggctgggta tacttgctaa
      541 aatctggcgg ccggatagcc ctccattaag gtcgcgttca agacgattaa cattattcaa
      601 tgcagttcgt aaagctctct ctgataatcc ttcagggctt acacttccca taaagaagcc
      661 taaaaagaca gaacgtattg taacaatagt gctccccatc tgaacctgag ctcctccgtt
      721 aaaagccagt gttcgtatgc cagaaagagt atcctgcggt atttccccca gtcccccact
      781 cgctgttaga tatggtgtta aatttattgg cataataaaa cccccattaa cttcattaac
      841 attttacttg atttcctgtt gaagcatctt acttatcaca atgctttcac acaccattat
      901 cagtcttgca aaagataaga ctccattatc aagccaaaat aaatttaact tcattaacaa
      961 acatcaccat gacatgacaa caaaaaaccg gagccagact ccggtttttt gaagctgtcg
     1021 ggttacttca tccagccaat attttcccac gtcccgtcag cacgcagaat ttgcagcggt
     1081 cttaccacgc actgtatctg ctttttatcc gcatccagta tcaccacctg cgtgattacc
     1141 ctggcctgct ccgggataat gccattctca t
//
LOCUS       DAZFWL020000414         1156 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFWL020000414 DAZFWL020000000
VERSION     DAZFWL020000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.420"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..497)
                     /gene="sctT"
                     /locus_tag="ACHKNP_RS27380"
                     /old_locus_tag="pgaptmp_005476"
     CDS             complement(<1..497)
                     /gene="sctT"
                     /locus_tag="ACHKNP_RS27380"
                     /old_locus_tag="pgaptmp_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HGU4707409.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFP"
     gene            complement(499..759)
                     /locus_tag="ACHKNP_RS27385"
                     /old_locus_tag="pgaptmp_005477"
     CDS             complement(499..759)
                     /locus_tag="ACHKNP_RS27385"
                     /old_locus_tag="pgaptmp_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4707410.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(769..>1156)
                     /locus_tag="ACHKNP_RS27390"
                     /old_locus_tag="pgaptmp_005478"
     CDS             complement(769..>1156)
                     /locus_tag="ACHKNP_RS27390"
                     /old_locus_tag="pgaptmp_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4707411.1"
                     /translation="TGMSGYKSYLIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNIS
                     IFSLLPAYALSEIKSAFIIGFYIYLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLI
                     LFVAMDGWTMLSKGLILQYFDLSINP"
BASE COUNT          407 a          251 c          184 g          314 t
ORIGIN      
        1 ttaggaaaat taaaattgcc tgaaggccat aaattataac tttgccaaag agtttccaga
       61 ataaagttca ttccaccttt agtaagataa accgcagcag agaaaaggtt aaataatctt
      121 gctaattccg aggtatcgac acctgtagcc ggatccaaag tactacttaa tgttgcaccg
      181 cgttggttat cgataaagct acctatagca aggaaaatcc aaaacgggga tgctaataag
      241 catgcaataa acagccctat gataatttct ctggcagcga acaaagcaat atcgatttca
      301 tttaagttca ataattcata gtggtaatgc tgaactaatg cacatgatac cactattatt
      361 attgggtgac gaactactgt tggtatattc ccactggcga aaaatggtaa caaatagaaa
      421 gttggtgcca atcggcaaaa accgagcgct gtagcagcca gcagactatg caactgatag
      481 agtatcgcct cccccatgct atcctctcgc gaatgctaaa ttcagcattt cgataccaaa
      541 tgaatataat ttttcaccat accacccgct cattaaaaaa aagcaaatac ttacgcacaa
      601 taatttcacg ccaaatggca acgtctgttc ttgcaactgc gttaccgttt gaaaaagccc
      661 taccaataaa ccaacaaatg tcgcaactgc aataggtcct gcagacataa ctaaaatcag
      721 atacagcgcc ctattccccg caaaaacaat atcgtccata tttttttctc agggattaat
      781 ggataggtca aaatattgta aaatgagccc ttttgataac attgtccaac cgtccatcgc
      841 gacaaacaaa attaatttta tcggtgtaga tatggttacc ggactcatca tcatcatacc
      901 taacgttaac agaacgctgg atataaccag atccactacg acaaagggta aatagatata
      961 aaaacctata ataaatgcac tttttatctc actcaatgca tatgcgggca gtaacgagaa
     1021 aattgaaata ttatcatcat cgattatctc ttcattgtct tcagaactat tcaccttctg
     1081 aatcttttca aaaaagctaa ccaactcagg ttcagaatat tttatcaaat atgatttata
     1141 accactcatg cctgtc
//
LOCUS       DAZFWL020000415         1147 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFWL020000415 DAZFWL020000000
VERSION     DAZFWL020000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.421"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1147)
                     /locus_tag="ACHKNP_RS27395"
                     /old_locus_tag="pgaptmp_005479"
     CDS             complement(<1..>1147)
                     /locus_tag="ACHKNP_RS27395"
                     /old_locus_tag="pgaptmp_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HGU4707412.1"
                     /translation="GKNVNANIKHSQIQEIKRNFTYKKNDDISSRFKFYSSLVKQKNS
                     TEDVVLIGKMISDEVRNYSTIHNDRNIIINSGNWKTSFLCNLARLLYSMFNGSVYFPS
                     RDDENSSSPSPTLPTIHQSERKELSQQKSINHLKKSDTIDEYIKIRKTRGIEDQITTT
                     SQSLIINELLNGVNRNTIPFQKISELNDIIHSYENMQTKNSRKGIEILVKQGELLSSL
                     INYDKENKQLSDNISKIINLLGIEYQSHKVDIEPFIHAVWVAGAPPENTSSYITAFLN
                     TYKNYTYLLWIDPNAFGAAKFSGILKNIAMNYAIMRLRRTNPHLAEKMNDVILKIQHI
                     QNETVDFNETRERVKELENKYKSLTRETKEKFNVFFLESMIGMQDNY"
BASE COUNT          319 a          176 c          168 g          484 t
ORIGIN      
        1 aaatagttgt cttgcatacc tatcattgac tcaagaaaaa aaacattgaa tttttctttg
       61 gtttctctcg ttaatgattt gtatttattt tcaagttcct ttaccctctc tcttgtttca
      121 ttaaaatcta ctgtttcatt ttgtatgtgt tgtattttta atatcacatc attcattttt
      181 tccgctaaat gaggattggt tcttctcaaa cgcataattg cataattcat ggctatattt
      241 tttaaaattc cactaaactt ggctgcacca aaagcattgg gatcaatcca gagcaagtaa
      301 gtataatttt tatatgtatt taaaaaagct gttatatatg acgatgtatt ttcaggaggt
      361 gcacctgcaa cccatactgc atgtataaat ggctctatgt ctactttatg tgactgatac
      421 tctatcccta ataaatttat tattttagat atattgtctg ataattgttt gttttctttg
      481 tcataattta ttaatgatga taacagttct ccctgcttaa ctaatatttc tatacctttt
      541 cggctatttt tagtttgcat attttcatat gaatgtatga tgtcattgag ctcacttatt
      601 ttctgaaatg gaatggtatt tctattaact ccatttaaca gctcattaat tatcaaactt
      661 tgagaggtag ttgttatttg atcttcaatg cctcttgttt ttcttatttt aatgtattcg
      721 tcgatggtat cacttttttt taaatgattg atgctttttt gttgtgataa ctccttcctt
      781 tcagactgat gtatagtagg tagagttggg ctgggggatg agctattttc atcatcacgg
      841 gaaggaaaat agacgctacc attaaacatg ctatatagta gtctggcaag attacataaa
      901 aaagatgttt tccaatttcc agagttaatt atgatatttc tatcattatg tatggtgctg
      961 taatttctaa cttcatctga aatcattttt cctatcaaaa caacatcttc tgttgagttt
     1021 ttctgcttta ctagcgagct gtaaaattta aatctggatg agatgtcatc atttttttta
     1081 taggtgaaat tcctttttat ctcttgaatt tgactatgtt tgatattagc attaacattt
     1141 ttacctc
//
LOCUS       DAZFWL020000416         1131 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFWL020000416 DAZFWL020000000
VERSION     DAZFWL020000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.422"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1131
                     /gene="rhsB"
                     /locus_tag="ACHKNP_RS27400"
                     /old_locus_tag="pgaptmp_005480"
     CDS             <1..>1131
                     /gene="rhsB"
                     /locus_tag="ACHKNP_RS27400"
                     /old_locus_tag="pgaptmp_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HGU4707413.1"
                     /translation="VYDRSNTQVRSFTYDDKYRGRMVAHRHTSRPEIRYRYDSDGRVT
                     EQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFD
                     AVGRLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLE
                     IRREYDELGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYAQLLSF
                     TDCSGYVTRYDHDRFGQMTAVHREEGLSQYHAYDSRGQLIAVKDTQGHETRYEYNIAG
                     DLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRY
                     DVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYD"
BASE COUNT          287 a          332 c          336 g          176 t
ORIGIN      
        1 agtgtatgac cgcagcaata cacaggtgcg cagctttact tacgatgata aataccgggg
       61 ccggatggtg gcgcaccgtc acacgagccg accggaaatc cgttaccgtt acgacagcga
      121 cgggcgggtg acagaacagc taaacccggc aggcttaagc tacacgtatc agtatgagaa
      181 agaccacatc accatcaccg acagcctgga ccgccgtgaa gtgctgcaca cgcagggcga
      241 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
      301 gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga caacagaata
      361 cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg gcagggcatc
      421 ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg acgggctgga
      481 aatacgccgg gaatatgatg aattgggccg tctgattcag gaaactgccc ctgacggcga
      541 tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa cggaagatgc
      601 caccggcagc cggaaaacca tgacgtggag ccgttacgct cagttgctga gcttcaccga
      661 ctgttccggt tatgtaaccc gttatgacca tgaccgcttc gggcagatga cggcggtgca
      721 ccgcgaggaa ggcctgagcc agtaccacgc atacgacagc cgtggacagt taattgccgt
      781 gaaagacacg cagggccatg aaacgcggta tgaatacaac atcgccggtg acctgaccgc
      841 cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg gaaaggccgt
      901 ccgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg gacgggtcat
      961 ccgcctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac tcgaccggct
     1021 gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc tgaccggcaa
     1081 acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg a
//
LOCUS       DAZFWL020000417         1127 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFWL020000417 DAZFWL020000000
VERSION     DAZFWL020000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.423"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..347)
                     /locus_tag="ACHKNP_RS27405"
                     /old_locus_tag="pgaptmp_005481"
     CDS             complement(<1..347)
                     /locus_tag="ACHKNP_RS27405"
                     /old_locus_tag="pgaptmp_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HGU4707414.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQL"
     gene            complement(982..>1127)
                     /locus_tag="ACHKNP_RS27410"
                     /old_locus_tag="pgaptmp_005482"
     CDS             complement(982..>1127)
                     /locus_tag="ACHKNP_RS27410"
                     /old_locus_tag="pgaptmp_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGU4707415.1"
                     /translation="LSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNK
                     ENP"
BASE COUNT          330 a          233 c          191 g          373 t
ORIGIN      
        1 agttggagtg gatttagatt tttcagatat ggtcttatga tctttccctt acttaataaa
       61 tagcatgtat aggattcgga aagtccagca tctattttgt acctcattat attagataag
      121 gatgtgagaa attccttgtt atcattcgca gccatatatg ccagtccgag ttcagcctct
      181 gcggccttac aggcaaaata gcttagtgaa cacgcttttc gtccctcttt agtactggct
      241 gatattagtg cccataatat tgctttgtct gccaaatcta actctctctg aaattgtctt
      301 gcagtaactg cactcgcaga aatagagaaa aaaatacaat tgttaataac cttgccatat
      361 tttttagtcc gtaactgtta atgtgctatc tggctctgaa aatattaact catttaattt
      421 ttgcatgtca ttagataaac cagttataca accctcggaa cagaatcgag gtcctaaaga
      481 cggaccaaaa gcgtttaaac aactatgtat tgaaaatcca ttgcgattat aagttcctgt
      541 attaactgac ggtactaatc gtatgccgtc aggttttcta ttgcttgctc ccgggtcatt
      601 aacattctaa ctccataggc cttgaggtat tggccatcta tttataaatg tgtacaacgg
      661 ggattatttt tacattgcat attattacca gaagcgactg gaatactaag cacatcataa
      721 aaattatcat cacatggtat atctgaaaca cacgaaagca tacctaaagt tatactgtat
      781 aaacaggtgg ctaatcccaa ggggatcgat tccagaaatc gggttcaacg gatactgata
      841 aaaattccac ctaccttcca gcccaatcgg agcctgagtg ttataccgcc cctgctgcgg
      901 ttaatgttac cgataatagt tatagtacag ccctgattca tcatcatact actgctgcgg
      961 cagtctgatg aactattgct tttacggatt ctccttattc ttccttctat ccattcgctt
     1021 tagctttttg agctcttgaa gtttgctctt tgcatcaata ttaccgttat ttacagcgaa
     1081 ttccatccaa taaatagctc tattcaaatt ataatattct gataatg
//
LOCUS       DAZFWL020000418         1127 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFWL020000418 DAZFWL020000000
VERSION     DAZFWL020000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.424"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            38..823
                     /locus_tag="ACHKNP_RS27415"
                     /old_locus_tag="pgaptmp_005483"
     CDS             38..823
                     /locus_tag="ACHKNP_RS27415"
                     /old_locus_tag="pgaptmp_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707416.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(960..>1127)
                     /gene="ykfM"
                     /locus_tag="ACHKNP_RS27420"
                     /old_locus_tag="pgaptmp_005484"
     CDS             complement(960..>1127)
                     /gene="ykfM"
                     /locus_tag="ACHKNP_RS27420"
                     /old_locus_tag="pgaptmp_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HGU4707417.1"
                     /translation="IVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNE
                     YISRKEDSAVK"
BASE COUNT          367 a          218 c          238 g          304 t
ORIGIN      
        1 attaccatga ttatgacagg cgagggagtg tccaattatg aattcaaaaa agctttgttg
       61 catatgtgtg ttattctcgc tgcttgcagg atgtgcctct gaatcttcta ttgatgaaaa
      121 gaagaaaaaa gctcaagtca cacaaagtaa tattaataaa aacactcccc agcaactgac
      181 agacaaagat ttattcggta atgaaaccac tctggccgta tccgaagagg atattcaagc
      241 tgcgcttgat ggagatgagt tccgcgttcc ccttaattct cctgtaatac ttgttcaatc
      301 cggcaaccgc gcaccggaaa ccattatgca ggaggagatg cgaaaatatt atactgtttc
      361 cacattctct ggtatcccgg acaggcaaaa gcctctgact tgtaacaaaa acaaagataa
      421 aaatgagaac gaggatgttg ccagtgctga gaatatgaac tggatgcagg cactgcgttt
      481 tgtggctgca aaagggcatc agaaagcgat tattgttttc caggatacgt tgcagacagg
      541 aaaatatgac tctgcgctga aatcaacagt atggtccgac tataaaaatg aaaaactcac
      601 cgacgctatc tccctgcgct acctggtacg tttcacgctg gtggatgtgg caacaggtga
      661 gtgggctacc tggtcgccgg tgaattatga atataaagtg cttccaccat tgcccgacaa
      721 gaatgaagcc agtacgactg atatgacaga gcagcaaatc atgcaactta agcaaaagac
      781 ctataaagcg atggtaaaag atttggttaa ccgctatcaa taataaatta tatctgccgc
      841 caggaatttc tggcggcaat aatacaaaat tctttgcata agaaattttt cttgacaaag
      901 acagtgcaag aaaggacatg gaatccggag ttaaaaactg tttgttttag aatatttaat
      961 tatttaactg ctgaatcttc cttgcgagaa atatattcat tattacattt catgccattt
     1021 ttaatataga ttgctcctgg gaaaatcccc gtattattac cgcaggggtg ataattagca
     1081 ttgacatatc ctgtgacaaa aggagctatt aaaggtgcta ttacgat
//
LOCUS       DAZFWL020000419         1116 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFWL020000419 DAZFWL020000000
VERSION     DAZFWL020000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.425"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            59..205
                     /locus_tag="ACHKNP_RS27425"
                     /old_locus_tag="pgaptmp_005485"
     CDS             59..205
                     /locus_tag="ACHKNP_RS27425"
                     /old_locus_tag="pgaptmp_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707418.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
     gene            433..639
                     /locus_tag="ACHKNP_RS27430"
                     /old_locus_tag="pgaptmp_005486"
     CDS             433..639
                     /locus_tag="ACHKNP_RS27430"
                     /old_locus_tag="pgaptmp_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4707419.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            704..928
                     /locus_tag="ACHKNP_RS27435"
                     /old_locus_tag="pgaptmp_005487"
     CDS             704..928
                     /locus_tag="ACHKNP_RS27435"
                     /old_locus_tag="pgaptmp_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707420.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          264 a          257 c          317 g          278 t
ORIGIN      
        1 tgctgattga ttttgtcctg gctgatacag gcaaagggaa aataacaaag gcggattgat
       61 ggggtggcta atgatatcag ataaactcat aacgctggtg aagagcctct gtgtacttgt
      121 cggcatttca tttttagtca tgctggttgc cattttcttt tccaccgcct ggcgagtcct
      181 gacgttatcg ggactggtgg ggtgaaagag agatgaaccg tgttctgtgt gtggtgatta
      241 ttgtcatggc ggttggctgt ggtgcgctgt ggctggcaac aaaccattac cgtgacaacg
      301 cgctcaccta caaagcgcag cgcgataaaa aagccagaga gctggaacag gcgaatgcca
      361 ccattactga catgcaggtg cgccagcgtg atgttgctgc gctcgatgca aaatactcga
      421 gggaattagc cgatgcgaga gctgaaaatg aaactctgcg tgctgatgtt gccgctggtc
      481 gtaagcgcct gcggatcaac gccacctgcc ccggtaccgt gcgtgaagcc accggcacct
      541 ccggcgtggg caatgatgcc gccgtcgaac tctctccggt tgctggacga aacgttctcg
      601 gtatcagaga cggaatcatc agcgaccagg cagcattgag aatgcttcag gaatatatcc
      661 gcactcagtg tattaactag tatttttgtt attcggagaa tgcatgaaga aattattggt
      721 aaccgtaaag ccttttcagg gaacaattcc gttccgtatt ttgcagcgtg gtcgtgttct
      781 tgttgaaggt tcgttcagtg gtaaatgtac gcaattacac tccaggacct ttcaggtgaa
      841 tgccacgaat gaagagctaa ccgttgagtg tacgatgaat gccgctaaat gccgcatggt
      901 atccgctgca ttacagccag tgtgttgagc gaccttatta ttcatgcgcg gtattgtcgc
      961 cgtattcctg cattaacaga gaccgcagcc cgaccgggag aatcctctgc gcgagtgtgc
     1021 ggggataatc aaaaacgata cacaccgggg tttaccgcgt taacggagcg cggcgttgtc
     1081 ccctcatggt cgctggtccg gtgcgatggt ggaaga
//
LOCUS       DAZFWL020000420         1104 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFWL020000420 DAZFWL020000000
VERSION     DAZFWL020000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.426"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1083
                     /gene="ompC"
                     /locus_tag="ACHKNP_RS27440"
                     /old_locus_tag="pgaptmp_005488"
     CDS             <1..1083
                     /gene="ompC"
                     /locus_tag="ACHKNP_RS27440"
                     /old_locus_tag="pgaptmp_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU4707421.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYTRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTQANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGKNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          317 a          228 c          245 g          314 t
ORIGIN      
        1 atgaaaaaat taacagtggc aatttctgct gtagctgcat cagtactgat ggcgatgtct
       61 gctcaggcag ctgaaattta taataaagac agtaacaagc tggatctgta cgggaaagtt
      121 aatgctaagc actacttctc ctctaatgat gcagatgatg gtgatactac ttatacccgt
      181 cttggcttca aaggtgaaac ccaaatcaac gatcaactga ctggtttcgg tcagtgggaa
      241 tatgaattca aaggcaaccg cgctgaatct caaggttcct ccaaagataa aacccgtctt
      301 gccttcgctg gcctgaaatt cggtgactac ggctccatcg attatggccg taactacggt
      361 gtagcatacg atattggtgc atggaccgac gttctgccag aattcggtgg cgatacctgg
      421 acccaaacag atgtattcat gactggtcgc accacaggtg ttgcaactta tcgtaacaat
      481 gacttctttg gtctggttga tggtctgaac tttgctgctc agtatcaggg taaaaatgac
      541 cgtactgacg taactcaagc taatggtgat ggtttcggtt tctccactac ttatgagtat
      601 gaaggtttcg gcgtaggtgc aacctatgct aaatctgatc gcactaataa tcaggttatc
      661 tacggtaaca acagcctgaa tgcatctggt caaaatgctg aagtatgggc agctggtctg
      721 aaatatgatg cgaacaacat ctatctggct actacctatt ctgaaaccca aaacatgact
      781 gtttttggta aaaaccatat tgccaacaaa gcacaaaact tcgaagtagt tgcacaatat
      841 cagttcgact tcggtctgcg tccgtccgtt gcttatctgc aatctaaagg gaaagacttg
      901 ggtgcttggg gtgatcagga cctggttgaa tatattgatg taggtgcaac ctattacttc
      961 aacaaaaata tgtccacttt tgttgactac aaaatcaacc tgattgataa gagcgatttc
     1021 acgaaagcat ctggcgttgc taccgatgat atcgttgctg taggtatggt ttaccagttc
     1081 taatttgatt actaaaacat atgt
//
LOCUS       DAZFWL020000421         1101 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFWL020000421 DAZFWL020000000
VERSION     DAZFWL020000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.427"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..186
                     /locus_tag="ACHKNP_RS27445"
                     /old_locus_tag="pgaptmp_005489"
     CDS             <1..186
                     /locus_tag="ACHKNP_RS27445"
                     /old_locus_tag="pgaptmp_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EspF repeat-containing protein"
                     /protein_id="HGU4707422.1"
                     /translation="QHLAEHGIQPARNMAEHIPPAPNWPAPTPPVQNEQSRPLPDVAQ
                     RLMQHLAEHGINTSKRS"
     gene            complement(313..582)
                     /locus_tag="ACHKNP_RS27450"
                     /old_locus_tag="pgaptmp_005490"
     CDS             complement(313..582)
                     /locus_tag="ACHKNP_RS27450"
                     /old_locus_tag="pgaptmp_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4707423.1"
                     /translation="MNILRKLMERLCGCGKHDDCEHGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENVGGMK"
     gene            complement(584..>1101)
                     /locus_tag="ACHKNP_RS27455"
                     /old_locus_tag="pgaptmp_005491"
     CDS             complement(584..>1101)
                     /locus_tag="ACHKNP_RS27455"
                     /old_locus_tag="pgaptmp_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4707424.1"
                     /translation="PKGDKGERGDTGPVGATGERGPTGDAGPAGPQGPKGDRGERGET
                     GLTGNAGPQGPKGDTGAAGPAGPQGPKGETGAAGPVGATGPQGPKGDPGETQIRFRLG
                     PASIIETNSHGWFPDTDGALITGLTFLDPKDATRVQGFFQHLQVRFGDGPWQDVKGLD
                     EVGSDTGRTGE"
BASE COUNT          213 a          371 c          242 g          275 t
ORIGIN      
        1 cagcatcttg cagagcatgg cattcaacca gcccggaata tggctgaaca tattcctccg
       61 gcacctaact ggcctgcgcc aacgccgccg gtacaaaatg aacaatcaag acctctgcct
      121 gatgtggctc agcgtctgat gcagcatctt gcagagcatg gcattaatac atctaagcgc
      181 tcgtgattat aatataatta cctgtattag ctctgacctg acagttaccg gttatttata
      241 caggtatctg tcagattaca tctaaccaac aaaaaaccgg agccggactc cggtttttgt
      301 gaagctgtcg ggctatttca tcccgccaac attttcccac gtcccgtcag cacgcaggat
      361 ttgcagcggt cttaccacgc actgtatctg ctttttatcc gcatccagta tcaccacctg
      421 cgtgattacc ctgtcctgct ccgggataat gccattctca tctgactcca ggatgtctgc
      481 cggccccagt cgcagctgtg ctgtaagtaa ctccccgtgt tcacagtcat catgctttcc
      541 gcaaccacac agacgctcca taagctttct caaaatattc atgtcattct cctgttctgc
      601 ctgtatcact gcccacttca tccagtccct taacatcctg ccacggcccg tcgccaaacc
      661 tgacctgcaa atgctgaaaa aaaccctgaa cccgtgtggc atctttgggg tcaagaaagg
      721 tcagtccggt gatgagtgcg ccatctgtat ccgggaacca gccatggctg tttgtctcaa
      781 taatgctcgc cggccccaga cgaaaacgga tttgtgtctc ccccgggtcg cccttcggtc
      841 cctgaggtcc ggttgccccc accgggccag ccgcacctgt ttctcctttc ggtccctgtg
      901 ggccagccgg gcctgccgca ccggtatctc cctttggacc ctgtggacct gcatttcccg
      961 tcaggccggt ctctccccgc tctcccctgt cgcctttcgg tccctgtggt cctgccggac
     1021 cagcatcacc tgtcggcccc cgttcgccgg ttgccccgac agggccggtg tcaccacgct
     1081 ctcccttatc ccccttcggc c
//
LOCUS       DAZFWL020000422         1100 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFWL020000422 DAZFWL020000000
VERSION     DAZFWL020000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.428"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            93..962
                     /locus_tag="ACHKNP_RS27460"
                     /old_locus_tag="pgaptmp_005492"
     CDS             93..962
                     /locus_tag="ACHKNP_RS27460"
                     /old_locus_tag="pgaptmp_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HGU4707425.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT          335 a          241 c          245 g          279 t
ORIGIN      
        1 gttattttaa caactattac taccaagccc atttatctgg cttataaatt aaaattaatt
       61 atttaattga gaaatggtta gggagaacct acatggccat aagcatcaag ggtgttaata
      121 ccggtgttat tcgcaagagt aataatttca ttgcactggc gctgaaaatt aaagaacctc
      181 gcaataaaga atcgttgttc tttatgtctg tcatggaact gcgcgacctg ctgattgcgc
      241 tggaaagtcg tatgcatcaa aagcataaac tggatgctgc tgcacgcctt cagtatgagc
      301 aagctcgcga taaagtcatt aagaaaatgg cagaaaacat cccagaaatt ctggttgatg
      361 agcttaaaaa tgccgatatt aatcgtcggg ttaacacgct ggaacttacc gataatcaag
      421 gcgaaaatct taccttcgta ttaacgctac atgacggtag caaatgtgag ttagtcgtca
      481 atgagttgca gattgaaatg ctggcgcgag caatcattca tgcaattaat aatgcggaaa
      541 tgcgtgaact ggcattacgt attacctcac tgttagattt cctgccgtta tatgacgttg
      601 actgccagga aaatggcaat ctggaatatg acacctattc ccaaccggaa tggaaacata
      661 acctgttcga ccattatctg gcggtgcttt accgctttaa agatgaaagt ggcaaagaac
      721 aatttagcgg cgcagtggtg aaaacgcgtg aagcaacgcc gggtaaagaa atagaggcga
      781 tcactcgccg aatgcttgat ttcagccaac gactgaaaaa actcgcgggt gtgccatgtc
      841 aggtctatgt tcgcaccgta gcggcgaata acgcccaacc gctaacccag gatcaatgct
      901 tacgcgcgct gcatcactta cgtgttcaat ccaccagcaa aactgcacca caggcgaaat
      961 aatctcatct ttgctaatgc gaggctggag ccatccagcc tgaaatgact gataacgtga
     1021 tcgttggtca tgccggatgc ggcgtaaacg ccttatccgg cctacaaatt ctcaagcatt
     1081 caatcagttg caatgcctgc
//
LOCUS       DAZFWL020000423         1100 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFWL020000423 DAZFWL020000000
VERSION     DAZFWL020000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.429"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..197)
                     /locus_tag="ACHKNP_RS27465"
                     /old_locus_tag="pgaptmp_005493"
     CDS             complement(<1..197)
                     /locus_tag="ACHKNP_RS27465"
                     /old_locus_tag="pgaptmp_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707426.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASIDVLRKAVFEGANIIITH"
     gene            complement(220..1059)
                     /locus_tag="ACHKNP_RS27470"
                     /old_locus_tag="pgaptmp_005494"
     CDS             complement(220..1059)
                     /locus_tag="ACHKNP_RS27470"
                     /old_locus_tag="pgaptmp_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HGU4707427.1"
                     /translation="MNDIENYGNRYHVLQSFPMPQLASSREIRILLPASYYESDVSYP
                     VLYMHDGQNLFDKSVSFGPHVWDIPEAVDAFFPNSQYDGVIIVGIDNASSHGKFSRMD
                     EYSPWPLNTSFPLPGWDPDVDYSGGKGALYVDFIVNTLKNYIDSNFRTLPDRNNTAIA
                     GSSMGAYISLFAAILRLDVFSKVGVFSPALWFNDSAMLNFIQENNIFADLTVYLDVGT
                     LETSGMREDFPEVYISGAEKLCVSLRKQRNVTIDYHLWGGDTHSESAWAKRFPEMLKL
                     FYC"
BASE COUNT          369 a          237 c          190 g          304 t
ORIGIN      
        1 tcatgagtaa taatgatatt ggcaccttca aaaacagcct ttcttaaaac atcaattgag
       61 gcaatccagc aggtcccaat tttattaata agtgtcgcct cagcaccaaa aataatttta
      121 tcaacagttg gttgcgaaac ggcaaccatt gaatttaaaa aacaattaag atctgcggct
      181 gttttaatat gatgcattca acatccttca ccggtttcat cagcaataaa ataatttcaa
      241 catttctgga aaacgttttg cccatgcaga ctcagagtgg gtatcacctc cccatagatg
      301 ataatctata gttacattgc gttgtttacg gagactcacg cataattttt ccgcgcctga
      361 aatatagact tcaggaaagt cttcgcgcat accactggtt tccagcgtac cgacatcgag
      421 atatacagta agatctgcaa agatattgtt ttcctgaata aaattcagca ttgcgctgtc
      481 attgaaccat agtgccggcg aaaaaacgcc aacttttgaa aaaacgtcta gacgaagaat
      541 tgcggcaaac aaactgatgt acgcacccat agaactacct gctatcgctg tattattacg
      601 atctgggaga gttcgaaaat tgctgtcaat atagttcttg agcgtattca caataaaatc
      661 aacatacaac gcaccttttc ctccactata atccacatca gggtcccagc caggtaaggg
      721 aaaagatgtg tttagcggcc atggagaata ctcatccata cggctgaatt taccatgaga
      781 cgatgcatta tcaatcccca caatgataac accgtcgtat tgggaatttg ggaaaaaagc
      841 atctaccgcc tcaggtatat cccatacatg tggaccaaaa gatacagatt tatcaaataa
      901 attttgccca tcgtgcatat acaatacagg gtaactgaca tcagattcat aataggacgc
      961 aggtagtaat attcttattt cgcgactact tgccaattgt ggcatcggaa aggattgcaa
     1021 cacatgatat cgattcccat agttctcaat atcgttcata atttaaatta acctcaataa
     1081 acaaaagaaa tattacatct
//
LOCUS       DAZFWL020000424         1092 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFWL020000424 DAZFWL020000000
VERSION     DAZFWL020000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.430"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1092
                     /locus_tag="ACHKNP_RS27475"
                     /old_locus_tag="pgaptmp_005495"
                     /pseudo
     CDS             1..1092
                     /locus_tag="ACHKNP_RS27475"
                     /old_locus_tag="pgaptmp_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
BASE COUNT          387 a          150 c          266 g          289 t
ORIGIN      
        1 gtgagtattc aaagtttgct tgattatatt tcagtgatcc ctgatatacg acaacaagga
       61 aaggttaaac ataaattatc tgctattttg tttctcaccg tatgtgcagt aattgcaggt
      121 gccgatgagt ggcaggaaat tgaagatttt ggacatgaaa gacttgaatg gctaaagaaa
      181 tatggtgatt ttgataatgg cattccggtc gatgacacca ttgcacgcgt tgtgagtaac
      241 attgacagtt tggcctttga aaagatgttt attgaatgga tgcaggagtg ccatgaaatc
      301 actgatggtg aaattatagc aatagatgga aagaccataa gaggctcctt tgataaggga
      361 aaaagaaaag gagcaatcca tatggtgagt gcattctcga acgaaaatgg tgttgtactg
      421 gggcaggtga aaacggaagc caaaagtaat gagattacag ccattccaga gttgcttaac
      481 ctactgtatt taaagaaaaa tttgataacc attgatgcta tgggctgtca gaaagatatc
      541 gcttcgaaga tcaaagataa aaaagcagat tatcttctgg cagtaaaagg caatcagggg
      601 aaattacatc atgcattcga ggaaaaattt cctgtaaatg tgttttctaa ttataaaggc
      661 gattcgttta gtacgcagga gataagtcat ggaagaaaag aaacacgttt gcatattgtc
      721 agtaacgtaa cgcctgaact tttgtgattt tgaattcgaa tggaagggat taaaaaagct
      781 ttgtgtagca ttgtcattca ggcagaagaa agaagataaa tcagcagaag gtgtaagcat
      841 ccgatattat atttcatcaa aggatatgga tgctaaagaa tttgcacatg ctatcagagc
      901 gcactggctg atcgagcaca gtcttcattg ggtgttagat gtaaaaatga atgaagatgc
      961 cagccggata agaagaggaa acgcagccta aataatatct ggaataaaga agatggcact
     1021 gaatttatta agggattgca aagacattaa gggtgaagag gaaaagaaag aaggctgcgt
     1081 taaacacagg ga
//
LOCUS       DAZFWL020000425         1082 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFWL020000425 DAZFWL020000000
VERSION     DAZFWL020000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.431"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..98
                     /locus_tag="ACHKNP_RS27480"
                     /old_locus_tag="pgaptmp_005496"
                     /pseudo
     CDS             1..98
                     /locus_tag="ACHKNP_RS27480"
                     /old_locus_tag="pgaptmp_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
     gene            100..393
                     /locus_tag="ACHKNP_RS27485"
                     /old_locus_tag="pgaptmp_005497"
     CDS             100..393
                     /locus_tag="ACHKNP_RS27485"
                     /old_locus_tag="pgaptmp_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HGU4707428.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(454..1082)
                     /locus_tag="ACHKNP_RS27490"
                     /old_locus_tag="pgaptmp_005498"
                     /pseudo
     CDS             complement(454..1082)
                     /locus_tag="ACHKNP_RS27490"
                     /old_locus_tag="pgaptmp_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
BASE COUNT          347 a          199 c          176 g          360 t
ORIGIN      
        1 ataaacttaa cataaaaggt actgtttagt tgtttatagc actctgtgaa tgggataaac
       61 attattttaa actccaatct tgcgtaagag aaagttgata tgtcattagt aggatataat
      121 agtaacatca taataaccat cgcgctgata tcgttgctct ctgcatgcac cagagaaaat
      181 tcattaaagg agaatcaaaa taaaagaagt ggcgaatccc aagcgatagt agatgcaaga
      241 cagttcttta gcgaacaccc ggaatatata tcatcagcag aactggagca aaagctatat
      301 cgcgaatttg cacaggttac tacagttcca gcttataaag agatgagtat gtatcaattg
      361 cttatcattt cacaagatcg tttaactcat taaataagga tctacacgct ttttgcgtgt
      421 agattcttat aaatttctga ctacgacaga tgtttaattt tttaaattat cgtctttatt
      481 ctcgattttt gtgtcttcac cctcaatgaa tttatcttct gaagagagct gttcatcttg
      541 aacaggttgc ccagcattct ccacatcttc aagccacagc agaagacgta atatttcatc
      601 tatattttcg aagctaacat aatcgtagcg acgatgggta gcataatttt ttcgtgctaa
      661 ttttttatct gtgataattg gtattccgat ttcctttgca tatttcctta cgcctaaggc
      721 cacctcatta gttactctta cagaaatcaa tggaataggt gaaagatgtg gcttaaagta
      781 tatccctatt gcaatgtgag tagggttgga aatcatcaaa cgactattac tgacatcaga
      841 cttcaattgc tcagaaagaa tttcctgatg cgctctctgc gtttagactt aatttctgga
      901 tttccttctt gctccttgta ttctctttta acttcttgtt tatccatttt catatctttc
      961 ataaataaaa aatattcagc gataaaatca aaaattaaga caattatcat actgccgaga
     1021 caatacagta tgagaaggaa tagcatctct ccccaatctg ataataaaga tatgatatct
     1081 cc
//
LOCUS       DAZFWL020000426         1074 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFWL020000426 DAZFWL020000000
VERSION     DAZFWL020000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.432"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..375
                     /locus_tag="ACHKNP_RS27495"
                     /old_locus_tag="pgaptmp_005499"
     CDS             <1..375
                     /locus_tag="ACHKNP_RS27495"
                     /old_locus_tag="pgaptmp_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU4707429.1"
                     /translation="GATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVEKNIKVPL
                     TEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSN
                     NCYGQVSRRDQESALACWGIDR"
     gene            535..672
                     /locus_tag="ACHKNP_RS27500"
                     /old_locus_tag="pgaptmp_005500"
     CDS             535..672
                     /locus_tag="ACHKNP_RS27500"
                     /old_locus_tag="pgaptmp_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707430.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKYILIWLVFVIMASGYFIGQIRW
                     W"
     gene            674..1012
                     /locus_tag="ACHKNP_RS27505"
                     /old_locus_tag="pgaptmp_005501"
                     /pseudo
     CDS             674..1012
                     /locus_tag="ACHKNP_RS27505"
                     /old_locus_tag="pgaptmp_005501"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            984..>1074
                     /locus_tag="ACHKNP_RS27510"
                     /old_locus_tag="pgaptmp_005502"
     CDS             984..>1074
                     /locus_tag="ACHKNP_RS27510"
                     /old_locus_tag="pgaptmp_005502"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4707431.1"
                     /translation="MKPPAPPAWIMQPAPDWQTPLNGIISSSEN"
BASE COUNT          279 a          244 c          308 g          243 t
ORIGIN      
        1 ggagccaccc gggtggatgg taagcctgtt attcctggca tgaagctgtc gaaggggaaa
       61 tgcgaccagg ttaacgccat tgagcgtgat aaggcgctgg catgggtgga gaaaaacatc
      121 aaagtgccac tgactgaacc ccagaaagcg ggtattgcgt cattctgtcc ttacaacatt
      181 ggcccaggta agtgtttccc gtcgacgttt tataaacgaa ttaatgcagg agatcgaaaa
      241 ggtgcctgcg aagcgattcg ctggtggatt aaggacggtg gcagagactg ccgtattcgc
      301 tcaaataact gttatggtca ggtctcacgg cgtgaccagg agagcgcgct ggcgtgctgg
      361 ggaattgaca gataagcaga atattttgct gaaaaatgcg gtttgctcac acggacggat
      421 aacacaaaat cctgcgaact gacaaaaact aagtgaataa aagtaaaaac cccatttgtt
      481 ggctgcaagc ggggttttgt gtttcctgac tccggaaaag tcaaaggaga aagtgtgttt
      541 gattttagca aactgattcg ggagattcga gtgatggctg aaaaattatc aacctggaag
      601 tacatcctta tctggctggt gtttgtgatt atggcctccg gttatttcat cggtcagata
      661 cgctggtggt gaaatgaacc gcgtactgtg cgtggtcatc attgccctgc tggtggcctg
      721 tggtgcgctt agtctggggc tgaatcatta ccgcgataac gccatcacct acaaagcgca
      781 gcgcgataaa aaagccagag agctggagct ggcaaacgca actattactg atatgcaggt
      841 gcgccagcgc gatgttgctg cgctcgatgc aaaatactcg agggaattag ccgatgcgag
      901 agctgaaaat gaaactctgc gcgctgatgt tgccgctggt cgtaagcgcc tgcggatcaa
      961 cgccacctgc cccggtaccg tgcgtgaagc caccggcacc tccggcgtgg ataatgcaac
     1021 cggcccccga ctggcagaca ccgctgaacg ggattatttc atcctcagag aacg
//
LOCUS       DAZFWL020000427         1074 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFWL020000427 DAZFWL020000000
VERSION     DAZFWL020000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.433"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..882
                     /locus_tag="ACHKNP_RS27515"
                     /old_locus_tag="pgaptmp_005503"
     CDS             <1..882
                     /locus_tag="ACHKNP_RS27515"
                     /old_locus_tag="pgaptmp_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4707432.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGSYKGQPVTQLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     RFISPFEYAKINNPLKQ"
BASE COUNT          289 a          259 c          257 g          269 t
ORIGIN      
        1 ttccccttta tcggcaatcg ggatatcaca acactcaata ccccggacct gcttatccct
       61 gttcgtgctg cagaagctaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata
      121 tctgccgtaa tgcgttatgc cgtacagtct ggcatcatca gatataatcc tgctctggat
      181 atggctggcg cattgactac ggtaaaacgc cagcatcgcc ccgctcttga tctttctcgc
      241 ctgcctgaac ttttgtcgcg tattggcagt tacaaggggc aacctgtcac ccagcttgcc
      301 gttatgctga atttactggt ttttattcgt tccagtgaac tcagatacgc ccgctggtct
      361 gaaattgata ttgacaatgc catgtggact attccagccg aacgcgaacc tctgccaggc
      421 gtaaaattct cacaccgggg atccaagatg cgaacaccac atcttgtgcc actcagcaaa
      481 caggttgtag ccatactggc agaacttcag acatgggctg gtgaaaatgg tctgatcttt
      541 acgggagcac atgacccacg taaaccaatc agtgaaaaca ccgtaaataa agccctgagg
      601 gttatggggt atgacacaac ccaggatgtc tgtggtcatg gattccgggc gatggcatgt
      661 agtgcgttaa tagaatcagg tttgtggtcc cgcgatgcag ttgaacgtca gatgagccat
      721 caggaacgta acggtgtacg tgctgcttac attcataaag cagaacatct ggaagaacgt
      781 cgactgatgc tacagtggtg ggccgatttt ctggatgcaa acagagaaag atttatcagt
      841 ccatttgaat atgcaaagat taataatcca ttaaaacagt aatcatcccg ggcaaatgcc
      901 cgggaaccta ctccaggatt attttctttg ttaaaaaaac agacaaaacg gtattaactt
      961 cgtaagcgta cagcgagggc cgtattgacg gggatgtgtt attcagctgg cagtgctatg
     1021 cgccacggaa gcagttcgct gacccggttg accggccagt ctgctatgac gcca
//
LOCUS       DAZFWL020000428         1072 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFWL020000428 DAZFWL020000000
VERSION     DAZFWL020000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.434"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(113..802)
                     /locus_tag="ACHKNP_RS27520"
                     /old_locus_tag="pgaptmp_005504"
     CDS             complement(113..802)
                     /locus_tag="ACHKNP_RS27520"
                     /old_locus_tag="pgaptmp_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU4707433.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(817..939)
                     /locus_tag="ACHKNP_RS27525"
                     /old_locus_tag="pgaptmp_005505"
     CDS             complement(817..939)
                     /locus_tag="ACHKNP_RS27525"
                     /old_locus_tag="pgaptmp_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGU4707434.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT          289 a          266 c          198 g          319 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaacc cactcggcag
       61 cgggtttata cattttttac aacataccaa atttgcatga agtatatggc ttttaatcca
      121 gttttgcaat attttgctgt aaaaatgctg ccttttgttt tgaacgtgtt ctcgtcacaa
      181 gcaataaagc atcactatca agctgtagaa aaatgtgctt cattgcaacc cagcgttcag
      241 taaatgtctc ggaccagttt tttgttgtca ctcccaccaa tgatgccagt gtctggtatt
      301 cataggcctc acgccctgca agttcgctct tcacatcctg tgcagccagc cagattaacg
      361 tcttcaggcg atccagtgtc ttacctgcaa tttttctgtt acttaacaaa tctttaaact
      421 cgctccatgc ccattgcgtt atggtgacct gatgccccca tcgaacgctt tcgctgtaac
      481 accaaagcaa ccatgctttc tgatgttcat cgagagacaa aaccgcgcgg cgccatgaag
      541 aggttgagaa ttcaaccggg ctgaccaaag caatggatga accttttgcg tacgactgct
      601 taccggaagt cggcgtatta tccagcgtaa tcatcttgcc agttaccaca tccagaatgc
      661 gcggcttctt tcgtttgtat gtaccagtat caaattgtgc atgctcctgc caggcttcga
      721 gctggccttt cgttgctccg ttcaagtcag cagtagctgc cataagttgc tcacgaacat
      781 actgtaaata ttgggtattc atgcagtaaa tcctttctat attttggcat aattcttcaa
      841 cattcggtaa tcgttcaaaa ccgaatcggg gaaacgacat aagcacagga gcccccagcg
      901 acagcgaagg agttctgata tataagactc agacatcatt cattccccgg ttctccaata
      961 tctgtttcac tcatcatcca taactacctg taattgcccc cccttttttt gtaacagttc
     1021 ttatattgct atatagaata gccattacta atgcttttaa atttaataaa at
//
LOCUS       DAZFWL020000429         1070 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFWL020000429 DAZFWL020000000
VERSION     DAZFWL020000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.435"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            4..207
                     /locus_tag="ACHKNP_RS27530"
                     /old_locus_tag="pgaptmp_005506"
     CDS             4..207
                     /locus_tag="ACHKNP_RS27530"
                     /old_locus_tag="pgaptmp_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4707435.1"
                     /translation="MQSLTAQLRLGPADILESDENGIIPEQDRVITQVVILDTDKKLI
                     QCVVRPLQILRADGTWENIGGMK"
     gene            313..>1070
                     /gene="nleH"
                     /locus_tag="ACHKNP_RS27535"
                     /old_locus_tag="pgaptmp_005507"
     CDS             313..>1070
                     /gene="nleH"
                     /locus_tag="ACHKNP_RS27535"
                     /old_locus_tag="pgaptmp_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase NleH"
                     /protein_id="HGU4707436.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAAASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRAM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNSLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTNEVRCFNQYYGAGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFIDTTETNVLYDRTRN"
BASE COUNT          315 a          183 c          281 g          291 t
ORIGIN      
        1 cacgtgcagt cgcttacagc acaactgcga ctggggccgg cagacatcct ggagtcagat
       61 gagaatggca ttattccgga gcaggacagg gtaatcacac aggtggtgat actggataca
      121 gataaaaagc tgatacagtg tgtggtaaga ccgctgcaaa tcctgcgtgc tgacgggacg
      181 tgggaaaata ttggcgggat gaagtaaccc gacagcttca caaaaccgga gtccggctcc
      241 ggtttttgtt gtcatgtcat ggtgatgttt gttaagaaag taaagattga ttcattttga
      301 aggttgaaat gtatgctatc accatcttct gtaaatttgg ggtgttcatg gaattcttta
      361 accagaaacc tgacttctcc tgacagtcgt attttatcct ctgtaaggga tgcagctgct
      421 agctcggata atggggcgca agtaaaggtg ggcaacagaa catatcgtgt tgttgtcact
      481 gataataagt tttgcgttac aagagaaagt catagtggtt gctttactaa tctgttgcac
      541 aggctgggat ggcctaaggg agagattagc aggaaaattg aggtgatgct gaattcatcg
      601 ccagtgaata gggctatgga aagaggtgct gttcattcga atagacctga tttacctcct
      661 gttgattatg caccgccaga gttaccgagt gtggactata acagcttgcc tgtgcctggt
      721 aatgttattg gcaaaggggg taacgctgta gtatatgaag atgctgagga tgcaacaaaa
      781 gtcctgaaga tgtttactac atctcaaagc aatgaagagg taacaaacga agttcgttgc
      841 ttcaaccaat attatggtgc cgggagtgca gaaaaaatat atggcgataa tggcgatatt
      901 attggtatta gaatggataa aataaatgga gaatcgcttt taaatatttc gtccttgccg
      961 gcacaggctg agcatgccat ttatgatatg tttgacaggc ttgagcaaaa aggaattctt
     1021 tttatcgata caacagagac aaatgtctta tatgatcgca cgagaaatga
//
LOCUS       DAZFWL020000430         1066 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFWL020000430 DAZFWL020000000
VERSION     DAZFWL020000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.436"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(83..430)
                     /gene="tnpB"
                     /locus_tag="ACHKNP_RS27540"
                     /old_locus_tag="pgaptmp_005508"
     CDS             complement(83..430)
                     /gene="tnpB"
                     /locus_tag="ACHKNP_RS27540"
                     /old_locus_tag="pgaptmp_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4707437.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(427..>1066)
                     /gene="tnpA"
                     /locus_tag="ACHKNP_RS27545"
                     /old_locus_tag="pgaptmp_005509"
     CDS             complement(427..>1066)
                     /gene="tnpA"
                     /locus_tag="ACHKNP_RS27545"
                     /old_locus_tag="pgaptmp_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HGU4707438.1"
                     /translation="RSYLPDALRLRFEDKLTIRAIAQRLGLSHSTIHTLFQRFIASGI
                     AWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYSREFKVRLAKQALQP
                     GAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNP
                     FWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIREMKGGTR"
BASE COUNT          228 a          282 c          283 g          273 t
ORIGIN      
        1 agcctgctgt gcgatcagca gggccttcag ttgctcgatg tcgtcgggga gtgtgttgtt
       61 cattcccctg ttttatcacg ggttatatcc ggatgccagg ccgttctgtc cgtttgggat
      121 gttgccacgc gatcccctcc agtagcatgg ataactgagc tggcgtcagg tgcactttcc
      181 cttcccgggt taccggccag acgaagcggc cccgttccag gcgtttggcg aacaggcata
      241 acccgtcacg atcggcccac agtattttca ccattttgcc actgcggccc cggaagacga
      301 agatatgccc ggagaacggg tcatctttca gcgtgttctg caccttcgaa gccaggccgt
      361 tgaagccaca acgcatatct gtgatgccag cgatgatcca gattctggta ccggttggca
      421 gcgttatcat cgggtacctc ctttcatttc gcggattagc gcccgtaaca tttccggagt
      481 gagagggtca aacagtttta ccacacctga tttaagatgc agctcgcacc gtgggacgtt
      541 tccgggatca ctctcagggc actcatcagg cttgttacgc cagaagggat ttgtaactgg
      601 tctggtcggc tctggcgtat cagtcagagc caccgggaca ggcatgcatt cctgtatgtc
      661 atcatcgctc agtaagccgt cctcgtactg gcttttccat ttaaacagca ggttatcatt
      721 gataccgtgc tctctggcga tccgggcaac aacagcaccg ggctgtaatg cctgcttagc
      781 cagacggacc ttaaattcac ggctgtagct ggctcgccgt tcttttcgcc atgtgccttc
      841 gctgatttga ggctctgtta attccttctt tctgttggca taaaggatgg catcaagctg
      901 agctaatgaa actgaatcgg gcaatggcca tgcgataccg gatgcaataa atcgctgaaa
      961 aagcgtatgt attgtggaat gactgagacc tagacgctga gcgatggccc ggatggtcag
     1021 tttatcttca aatcttaaac gcagagcatc aggcaaataa gaacgg
//
LOCUS       DAZFWL020000431         1066 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFWL020000431 DAZFWL020000000
VERSION     DAZFWL020000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.437"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..663
                     /locus_tag="ACHKNP_RS27550"
                     /old_locus_tag="pgaptmp_005510"
     CDS             <1..663
                     /locus_tag="ACHKNP_RS27550"
                     /old_locus_tag="pgaptmp_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGU4707439.1"
                     /translation="DGRDYDPDVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVK
                     EAVSHIHVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPRG
                     TYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTF
                     SLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDA
                     GG"
     gene            666..992
                     /locus_tag="ACHKNP_RS27555"
                     /old_locus_tag="pgaptmp_005511"
     CDS             666..992
                     /locus_tag="ACHKNP_RS27555"
                     /old_locus_tag="pgaptmp_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4707440.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            1002..>1066
                     /locus_tag="ACHKNP_RS27560"
                     /old_locus_tag="pgaptmp_005512"
     CDS             1002..>1066
                     /locus_tag="ACHKNP_RS27560"
                     /old_locus_tag="pgaptmp_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707441.1"
                     /translation="MAISAGRLTQMISVLNPVLTR"
BASE COUNT          233 a          256 c          356 g          221 t
ORIGIN      
        1 gacggtcgtg actatgatcc cgatgttctg cagaaggcgg tgaatgatgc ggtcgcaaat
       61 attccgcagc cggcagacgg taaaagtctc accccggatg atgtgcgtcc gatgcttgaa
      121 cagatggtga aggaggcggt aagccatatt catgttccgc gtgatggtcg tgactacgat
      181 ccggatgttc tgcagaaggc ggttctggat gcggtgagtg ccctgccggc tccgcaggac
      241 gggcgtgatg ccacggctct ggaaatactc cccgccattg acgatcaaaa atcctttccc
      301 cggggcacgt atgccacaca ccagggcgga ctctggcggg cgtatgaaaa aacgcacggg
      361 atgcggggat gggaatgcct ggttgacggg gtggcggata ttgacgtcag catgacgggt
      421 gagcggttgt tctctgtggt ggtccggcag agcagtggcc agcgtacgga aaaaacattt
      481 tccctgccgg tgatgctcta ccgcggtgtg ttcagagccg gtgaaaccta ccaccccggc
      541 gatacggtga cgtggggggg ctcgctgtgg cactgcaaca gtatgaccga agataaaccc
      601 ggagaagctc attcatcagc ctggaccctg gctgcaaaac gtgggcggga tgcaggaggc
      661 tgaaaatgac ggcattactg acactggaag agatcaaggc acatctgcgt gtcgaccatg
      721 acgcggatga tgacatgctg atggacaagg ttcgtcaggc taccgccgtg ctgctggcct
      781 acattcaggg cagccgggat aaagtgatcc gtgaggacgg tgaactgatc ccgggcgagg
      841 cattaacccg gatgaagggg gctgccatgc gactgaccgg gatgctgtac cggaatccgg
      901 atcttgcgga gcgggaagaa ctgcttcagg gggagctgcc gttttctgtt tccgtgctga
      961 tttacgattt gcgttgtccg acggtgttat gaggaggggg aatggcaata tctgcaggtc
     1021 gtctgacaca gatgataagt gttctgaacc cggtgttaac ccgtaa
//
LOCUS       DAZFWL020000432         1059 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFWL020000432 DAZFWL020000000
VERSION     DAZFWL020000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.438"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..311)
                     /locus_tag="ACHKNP_RS27565"
                     /old_locus_tag="pgaptmp_005513"
     CDS             complement(<1..311)
                     /locus_tag="ACHKNP_RS27565"
                     /old_locus_tag="pgaptmp_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU4707442.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LR"
     gene            complement(376..974)
                     /locus_tag="ACHKNP_RS27570"
                     /old_locus_tag="pgaptmp_005514"
                     /pseudo
     CDS             complement(376..974)
                     /locus_tag="ACHKNP_RS27570"
                     /old_locus_tag="pgaptmp_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
BASE COUNT          243 a          325 c          252 g          239 t
ORIGIN      
        1 cgcaatgcct ccggacgggc atcatcctcc gtcatggcac cgagaaaatc attcagcgta
       61 ccgggttgag aatcttcata cacggtgatg gtcccggcat gtgacggcgg gaatccctcc
      121 accaacagaa taacgctgta ctgaccgtac tcaacgtcca tgctgtaacg cccggcttca
      181 tccggatttt ctgaggccag cgtgttcacc accaccgtgg tgctgttacg ttttgctttc
      241 agctggattg tgcagttctg taccggtttt cctgtgccgt ctttcagtac acctgaaatc
      301 tttactgcca tattcacccc acaaaaaagc ccgcctgaac cggcgggctg tcataacact
      361 gtgttacctg gctaatcaga acttataacc gacacccacg atgaacccgt cagtgcgcca
      421 gtcgccactg ccggaacctt cataagcaat atcaattgcc acggattcgg tcgggttaaa
      481 ctgcacgcca gcccccacgc cagagacgtg ttgctgtggc gaccgtcatc acttccggtc
      541 agcacgtcgt gcgttttccc cttgttgtca gttacgcgga gataatcccc ggaaaaagtc
      601 gacacacggc tgtaagccac gcccgccatc gcatacgcac tgaaccattc attcacgcgc
      661 acagacggcc ccgccatcac gctgaaccag cggttacgca cagaatcttc atgccagcgg
      721 gtatcgctgt aacgggtaag ctggcgattc ctgtctcctg catagctgaa tgacgtcacc
      781 agccccagcg tgtccgtaaa ctcataacgg tatttcacgt taatcccgtt aagattatcg
      841 ctaccgggag cgctcgtccg ggcatgaaga taccccgcgc tcagcgtggc ctgctgctca
      901 gacgcccatg caggcgcgcc ggatacggcc agacaaatgg ctgcggacaa aatggcggca
      961 taaagtttac gcataattac ctctcgcttt tctgcaataa aaaaggcgcc atttctggcg
     1021 cccgtatatg ggttataaaa ttcagctgat actgatgcc
//
LOCUS       DAZFWL020000433         1053 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFWL020000433 DAZFWL020000000
VERSION     DAZFWL020000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.439"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1053
                     /locus_tag="ACHKNP_RS27575"
                     /old_locus_tag="pgaptmp_005515"
                     /pseudo
     CDS             1..1053
                     /locus_tag="ACHKNP_RS27575"
                     /old_locus_tag="pgaptmp_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
     misc_feature    195..311
                     /locus_tag="ACHKNP_RS27575"
                     /old_locus_tag="pgaptmp_005515"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          248 a          251 c          330 g          224 t
ORIGIN      
        1 tgggcggcaa tttgttccat tgccccaaag attggctgta cgccggagac cctgcgtgtc
       61 tgggttcgcc agcatgagcg ggataccggg ggcggtgatg gtgggctcac cagcgctgaa
      121 cgtcagcgtc tgaaagagct ggaacgtgaa aatcgtgaac tgcgccgcag taacgatatc
      181 cttcgccagg cttccgctta ttttgcgaag gcggagttcg accgcctctg gaaaaaatga
      241 tgccactgct ggataagctg cgtgagcagt acggggtcgg accggtatgc agcgaactgc
      301 atattgcccc gtcaacgtat taccattgtc agcaacagcg acatcatccg gataaacgca
      361 gtgcccgtgc gcagcacgac gactggctga agagagagat acagcgcgta tacgatgaaa
      421 atcatcaggt gtacggtgtg cgtaaagtct ggcgtcagtt gttacgggaa ggaatcaggg
      481 tggccagatg tacagtggca cgtctcatgg cggttatggg acttgccggt gttctccggg
      541 gtaaaaaggt ccgtacgacc atcagccgga aagccgttgc cgcaggcgac cgcgtaaacc
      601 gtcagttcgt ggcagaacga cctgaccagc tgtgggtggc tgattttact tacgtcagca
      661 catggcaggg cttcgtctat gtggcgttta tcattgatgt gtttgccgga tacatcgtgg
      721 ggtggcgggt ctcatcgtct atggaaacga cattcgtgct ggatgcgctg gagcaggcgt
      781 tgtgggcccg tcgtccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      841 cactggccta tacggagcga ctaaaagaag ccggattact ggcatcaaca gggagtacag
      901 gcgactcgta tgacaacgcg atggctgaga gcatcaatgg tctttacaaa gcggaggtaa
      961 tacaccgtaa gagctggaaa aaccgtgcag aagtggaact ggccacacta acgtgggtgg
     1021 actggtataa caatcgacga ttgctgggaa ggc
//
LOCUS       DAZFWL020000434         1052 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFWL020000434 DAZFWL020000000
VERSION     DAZFWL020000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.440"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..321
                     /locus_tag="ACHKNP_RS27580"
                     /old_locus_tag="pgaptmp_005516"
     CDS             <1..321
                     /locus_tag="ACHKNP_RS27580"
                     /old_locus_tag="pgaptmp_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4707443.1"
                     /translation="TRRVNILKGEQRRVSGSARVSLKRHIWLRDAGQCCLCGRVVDLC
                     DSELDHRIALQFGGGNEETNLWTLCTECHRQKSVSETASGMPDPTLPEVSGGHGRADD
                     IIGL"
     gene            613..>1052
                     /locus_tag="ACHKNP_RS27585"
                     /old_locus_tag="pgaptmp_005517"
     CDS             613..>1052
                     /locus_tag="ACHKNP_RS27585"
                     /old_locus_tag="pgaptmp_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4707444.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPAVMS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTH"
BASE COUNT          249 a          259 c          322 g          222 t
ORIGIN      
        1 acccggcggg tgaatattct gaagggtgaa cagcgtcggg tcagtggcag tgcacgtgtt
       61 tccctcaagc gtcatatctg gctcagggat gccgggcagt gctgtctctg tggtcgtgtg
      121 gttgacctct gtgacagtga actcgatcac cgaattgcac ttcagttcgg tggtggtaat
      181 gaggagacga atctctggac gctctgtacc gaatgccatc gccagaagtc agtcagtgaa
      241 acggcgagtg gtatgccgga cccgacgctg ccggaggtgt ccggaggtca tggcagggca
      301 gacgatatca tcggactgtg acccgccccg ggggggggga tcatccggcg aaaaaaacga
      361 tcgccccgga caccgccccc cgtctcacgc agagaaaaaa ttcccgtttc aggccagtta
      421 acatgttaac tggctgtccg ggcatttttg cggtttttat ctttattatt cagtttgttg
      481 cgtggaaaaa atgttaacag gctttttcag caaatgttaa ccaggcagca gttaacattt
      541 gcggcatgag acgccgggaa aaatgggctg aaccataccc ggctgagtgc gttatggacc
      601 cgggaggagg ctgtgctgac aacgcaaaaa cgaaaatttg cgctggcgct catgtccggg
      661 aaaaacaaaa cagcgtcagc cattgccgct ggttattcgg cgaagaccgc cagggttaaa
      721 ggctcgcagc tggcaaaaga tccggaggtg cttgcgttta tagcccgtaa acaatgcgag
      781 acggtggagg tggatgaggt tcctgtttac cggcagaaaa aatcagagcc ggaggataaa
      841 ccccgtcgcc gtgaggcggc tgcaatacca cagccggacg aaacaaatcc ggagatgcca
      901 ccgcccgcgg tgatgtctcc tggtattgaa tatatggagg acggtcttcc cgatccggtg
      961 aaagcgatgg ggcgtcttct ggtggagaac attaataccg accccaggct ggcgctggat
     1021 gcggcttata agctggcgca gttcacgcac ca
//
LOCUS       DAZFWL020000435         1050 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFWL020000435 DAZFWL020000000
VERSION     DAZFWL020000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.441"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..919)
                     /locus_tag="ACHKNP_RS27590"
                     /old_locus_tag="pgaptmp_005518"
                     /pseudo
     CDS             complement(1..919)
                     /locus_tag="ACHKNP_RS27590"
                     /old_locus_tag="pgaptmp_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            896..1050
                     /locus_tag="ACHKNP_RS27595"
                     /old_locus_tag="pgaptmp_005519"
                     /pseudo
     CDS             896..1050
                     /locus_tag="ACHKNP_RS27595"
                     /old_locus_tag="pgaptmp_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          244 a          309 c          272 g          225 t
ORIGIN      
        1 tacccgccgt acacggtgtc gtactgggta gcgtatgtat ttttccagta atacgtcgtg
       61 tcaccacaaa cccacggcac atctgcagca ctgccaccat ggcactgcgc gttaaacacg
      121 gagaggtcag cacgaaactg tgtcagcatg gctgtaaaca gcgcaggttg ctgtacgtgg
      181 gtggcggcgc tcatgtcaaa ctcaccctgc atccagcaga cggccagcag aacgtttttg
      241 ggatttttct gcaatgccgc ttttgtgcgg gaaatcagat cctgatataa cggcttgccc
      301 accccccagc gtgccgaatc ctgactggcc cccgtggact cgctgaatgt cccctccgcg
      361 ccctgggtaa atgccgaacc accacgacag catggtacca gcaggatccc cgcgttattc
      421 gggatatacg ggagcagttt tttggcaata tgtaaaccct ggccgacaca gccgtactgc
      481 cctttgctca ggtcagccct cggatgattc agcgtactca tatcctgcac atcatgcaga
      541 cagtggtcag ccggaataat atcgttatat ctgcaggcag ccccgcccgg cgtcactgta
      601 ctgcggcgcg ccagctgttt aatgcgcgga tccggagcat cgtatgaatc cggcagcgga
      661 agcccttcac cgtaagccct tcaccgtaag ccatggcatt ggattgcccg gccagtacga
      721 tgacgtagta ccaatccggc tcagatgaag ggccgacctg tggctctcct tcaatagcca
      781 ccgcctgcat cagtgtgtac ggcgtaatgg caaccggtcc gccgtatggc tgccagccct
      841 ctttcagttt gtgtgtcagc ttttccgcaa ggtctgacgg cgacgccgcc ctgacaacat
      901 cgtaatgttt aatcgacatc gaatttctcc cgtgtagagg aacagagtta aaaagccgga
      961 agcggaatca aatcacagga tgaccatctg ccagtggcag gtcataaaaa aaaggctgcg
     1021 caatgcgcag ccagaactca caaggaaaat
//
LOCUS       DAZFWL020000436         1049 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFWL020000436 DAZFWL020000000
VERSION     DAZFWL020000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.442"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            145..>1049
                     /locus_tag="ACHKNP_RS27600"
                     /old_locus_tag="pgaptmp_005520"
     CDS             145..>1049
                     /locus_tag="ACHKNP_RS27600"
                     /old_locus_tag="pgaptmp_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HGU4707445.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSE"
BASE COUNT          366 a          200 c          201 g          282 t
ORIGIN      
        1 aaagataata aaaaccgcaa taaaaaacga ataaatcaat tgtctcacgc aattataaaa
       61 catcatattg attacgcacc ttgtattaca aactcatgta tgtaaaatac gcgcaccatt
      121 caaaaaaaag gaagacaata acatatgaaa aaaagtgtca tcgctggcgt ctttattgct
      181 ctgtcattta ccacgtgttc agctatcgcg aacagccttg cattatcatt agcaaatgat
      241 gatgcaggga agtttcaacc aatacttaat gatatttatg gcaataaaca tgaaaacaga
      301 gatgattact cacaaggctt atttctggga tatagccacg atatctcaga ctcgagccaa
      361 ttatctctcc atattgcgca agatatttac tctccatcag gcagtaataa aagacacaac
      421 acagctgtaa ctggagacag agcttttagt gcatacactc acactggtat tgaatggaac
      481 tcccttgcga atgactggat tcgctatcga ttaggtactg acataggtgt tgttggcccc
      541 gacgcaggcg gtcagaaagt acaaaataaa gctcatgaga ttattggggc agaaaaatat
      601 catgcatggg atgatcaaat agagaatcgc tacggttata ctgtaaaagg gatgctatcc
      661 atgacaccaa gtatggatat tttaggtgct aatgttggat tataccctga agtttctgct
      721 gttactggaa acttatttca atatgtagca tatggcgcaa ccattgccat tggtaatgat
      781 aaaaccttca attcggataa tggctttggt ctgctggctc cccgtggttt aatgcatatg
      841 tccgatacaa gcggattcaa atacaagatt tttgcaggta tggaaagacg agatgtcaat
      901 cgcaactata ctctcgaagg aaaaacaata cagacgaaac aaacaacagt atcgctaaac
      961 aaaactgttg atgaatatca agttggcgca acaattgggt atgcacctgt agccttcaca
     1021 ctagcattta ataaagtaac atcagaatt
//
LOCUS       DAZFWL020000437         1036 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFWL020000437 DAZFWL020000000
VERSION     DAZFWL020000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.443"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..251)
                     /locus_tag="ACHKNP_RS27605"
                     /old_locus_tag="pgaptmp_005521"
     CDS             complement(<1..251)
                     /locus_tag="ACHKNP_RS27605"
                     /old_locus_tag="pgaptmp_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HGU4707446.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQR"
     gene            432..584
                     /locus_tag="ACHKNP_RS27610"
                     /old_locus_tag="pgaptmp_005522"
                     /pseudo
     CDS             432..584
                     /locus_tag="ACHKNP_RS27610"
                     /old_locus_tag="pgaptmp_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
BASE COUNT          334 a          171 c          198 g          333 t
ORIGIN      
        1 cgttgtgata tttttgttcc ggaacttgag gcactaaaag caacattaat agaacctggg
       61 ccagggaaat ggtatttaat aagtctgtaa ggatcaactt ttgcagagat atcgcttagg
      121 ccgagtgaga gaaagtcttg ttctaccata tcaccatatt gcatatcatc agcagaaaaa
      181 tcatcgaata aactaatagt ttcgaaaagt gtacagggaa tattaattgg cggaagtaat
      241 atgttattca tttgcatttg ccttggttat atttaagctc aatatcaatt cgagaacaaa
      301 tgctagggta aaaaaatatt taaaaaagaa gtaaacttca cattttttca ttacatctct
      361 gaagagaacc tcgttaatga ttggtcttgc tcattgttaa taacaaaatg ttgaatttac
      421 cgattaggaa actttttcct acgcggattg cctccagcta tggcatgaac tttgagttta
      481 tgccagaact gaagtggggc ttcggctacc ctggcgcgat tatctttatg atcctcgcgg
      541 gcctgacacc gtatctgtac tttaagcgta agaactggct gtaactatac gatgtcgctg
      601 tgagtaagtt agtgtgctga cagcgacatt ttagccgagg gaatcgcaga taacataatt
      661 gttgtgaata atatccatgc cacgttgagt tggacgctta atgactcaca tctcacccct
      721 tcaattgctg cagagataat agaaaaaagt gtcaagccca atgaaatcaa tacaaagtag
      781 attgccttac atccagtatc tcttattctt tttgctccaa tattgatatt tacataggta
      841 ttattaataa ccagaataaa actcaaagcc aggttgatat cattcaaagt tattaatatt
      901 gaccatgatg ttaatgtata gattatgaac tgaaagcgtg ataaacgctc aagcctggat
      961 gggtagaata tatttctgat ggatgatttg aacattttta cattgtttta tatataattg
     1021 ttttgaatat atatta
//
LOCUS       DAZFWL020000438         1017 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFWL020000438 DAZFWL020000000
VERSION     DAZFWL020000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.444"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..162
                     /locus_tag="ACHKNP_RS27615"
                     /old_locus_tag="pgaptmp_005523"
     CDS             <1..162
                     /locus_tag="ACHKNP_RS27615"
                     /old_locus_tag="pgaptmp_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707447.1"
                     /translation="ANIIIDSEYKSNKAVKEYVEILKEFNICPEKVAEYLSEFSGKHF
                     LDVYNYYSN"
     gene            344..529
                     /locus_tag="ACHKNP_RS27620"
                     /old_locus_tag="pgaptmp_005524"
     CDS             344..529
                     /locus_tag="ACHKNP_RS27620"
                     /old_locus_tag="pgaptmp_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGU4707448.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRTIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            770..>1017
                     /locus_tag="ACHKNP_RS27625"
                     /old_locus_tag="pgaptmp_005525"
     CDS             770..>1017
                     /locus_tag="ACHKNP_RS27625"
                     /old_locus_tag="pgaptmp_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
                     /protein_id="HGU4707449.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKI"
BASE COUNT          360 a          174 c          181 g          302 t
ORIGIN      
        1 gcaaacataa ttattgatag tgagtacaag tcaaataagg cagtaaagga atatgtagaa
       61 attttaaaag aattcaatat atgcccagaa aaggtagcag aatacctaag cgagttcagc
      121 ggaaaacatt tcctcgatgt atataattat tattccaatt gaaaaaaata taaagtatct
      181 atcacggcac tctcagttga gtatcacagg cacatgtatt ttagccccct gagtctacag
      241 acaaccaata ttacttaaat aagtgataat cttaatgctg ttttttagaa cagatgattg
      301 atatttttgg gccggagaaa cgcagacagg ctttgttgaa taaatcgagc ttttgctgag
      361 tttaaggatc agatcactca ttttcctgca aaagctcatt gggcgaaaca atctgactca
      421 gagaacccgg attaaacgtc tggcccgcag aaccatctat ttctcacgca ctgtagtacg
      481 taataaaaaa gtcatcggta cttttatcaa aaaacatata ttctattaat tattgccatc
      541 acctttttct tattgacaac attactctgc tgattgttaa aatctaacct gtattcaggg
      601 ttacgattct atacaataga acagggaggg gaaaggatgt catcgttaat gagacttttt
      661 gttaatgtca acaatgctat gaatttattt tagaaaaaca attaagctga cgtagtgttc
      721 cgtctaaatg acggggcgat attaacatga ttagaacaag aggaatttta tgttatcttc
      781 attaaatgtc cttcaatcca gcttcagagg aaagacagcc ttatcaaata gtacacttct
      841 ccagaaagtt tcttttgctg gaaaagaata tcctctggaa cctattgatg aaaaaacccc
      901 tattcttttt cagtggtttg aagcaaggcc agagcgatac gaaaaaggag aagtaccaat
      961 attgaatacc aaagaacatc cgtatttgag caatattata aatgctgcaa aaataga
//
LOCUS       DAZFWL020000439          996 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFWL020000439 DAZFWL020000000
VERSION     DAZFWL020000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.445"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..563)
                     /locus_tag="ACHKNP_RS27630"
                     /old_locus_tag="pgaptmp_005526"
     CDS             complement(<1..563)
                     /locus_tag="ACHKNP_RS27630"
                     /old_locus_tag="pgaptmp_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4707450.1"
                     /translation="MTETESAILAHARRCAPAESCGFVISTPEGDRYQPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDACHLAGITLPDFHREDEWWRNGQNLYLDNMEAT
                     GFYRVPLSSAQAGDILLCCFGASVPNHA"
     gene            complement(568..>996)
                     /locus_tag="ACHKNP_RS27635"
                     /old_locus_tag="pgaptmp_005527"
     CDS             complement(568..>996)
                     /locus_tag="ACHKNP_RS27635"
                     /old_locus_tag="pgaptmp_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4707451.1"
                     /translation="MAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELSDR
                     WVVEQMSELTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADE
                     FDNPTTDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
BASE COUNT          210 a          345 c          256 g          185 t
ORIGIN      
        1 gcatggttgg gtaccgatgc gccaaagcag cacagcagga tatcgcctgc ctgtgccgag
       61 gacaggggca cccggtaaaa accagtggcc tccatattgt ccagatacag gttctgacca
      121 ttgcgccacc actcatcctc gcggtgaaaa tccggcagcg ttatccccgc cagatggcag
      181 gcatccctga acagcgtgta acagtccgtc accccgtgtt caaaacgacg cccggtcagg
      241 tgcggcacac agcggaattt atgaatgtcc ccccggcaga ccagccacca gggcagggca
      301 ctttttatct gcagccgccg gtcggcctcg ctcagccagg gcaggccacc gggatggctg
      361 tggaccagtg ccacaatctc cccctgcatc tctgcccgca gccagtcttc cggggcaata
      421 cgaaaatatg cctcaggctc tgcagagata ttcacgcacg gctggtaccg gtcaccctcc
      481 ggcgtgctta tcacgaagcc gcaagactcc gcaggcgcac accgccgggc atgcgccaga
      541 atcgctgatt ctgtctctgt catggattta ctgcgaaagt ttattaatgg aaaggaaacc
      601 gccaaaatta gccaccatgc cgcgcatctc acacccgcgc atgcacttgc tgcatctgtc
      661 cttacggata tccgtggtgg ggttgtcgaa ctcatccgcc accgcaggac cgttataccc
      721 gcattcatct ccccggtaat cccacataca ggtgttcgcc agcatgatgc gaccaggaaa
      781 cagcgccccg tccgtctccg tcggtgttgc cagcacaaac gaggctgtca tggccgtcag
      841 ctctgacatc tgctccacca cccagcggtc gctcagctcc tgctccgggt ccgcttccgg
      901 attgcccgcc acaaaattca ccgcatccag aaaacgggca tacacccggc ggcggaccac
      961 cgtggccccc accaggctct gcaggtcctc cgccat
//
LOCUS       DAZFWL020000440          989 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFWL020000440 DAZFWL020000000
VERSION     DAZFWL020000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.446"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..854)
                     /locus_tag="ACHKNP_RS27640"
                     /old_locus_tag="pgaptmp_005528"
     CDS             complement(<1..854)
                     /locus_tag="ACHKNP_RS27640"
                     /old_locus_tag="pgaptmp_005528"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGU4707452.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPEPGMPSQMAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTYLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGASAQSITSFHKEKSAMNTGEQIKSFENKRAALAASLEEIMN
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELETSKAATAQPVKQAGNGNVAT
                     VASAPVIRV"
BASE COUNT          206 a          305 c          258 g          220 t
ORIGIN      
        1 acacggatca ccggcgctga agccaccgtg gccacattcc cgttaccggc ctgtttcacc
       61 ggctgtgccg tggcggcctt actggtttcc agttcacgca gacgcttcag gtgcgcatcc
      121 acctgacgga tttccgctgc ggtgttgtcg tagtgctctt cctcttccac atccagtgtg
      181 cggccttcct ctgcggcttt gttcatgatc tcctcaaggg aggctgccag cgccgcacgc
      241 ttgttttcaa aacttttaat ctgttcacca gtattcattg ctgacttttc cttatgaaaa
      301 gaggttattg actgtgccga agcgccggca gaagatgcga ttttcaccac cggtttccgg
      361 ttgccggacg cggcagaaaa cgggcggtcg taagatttaa tggtccggat ggtgcattcc
      421 gcattcgcgg gcacggtgac ggcagacacc tccatcagct cccagcgcag aaaatgcagt
      481 ccgcctccgt ccagataagt gtattcatgg ggccggaagc ccacagaaag ccccctgacc
      541 agcccggtct taatggcagc ccaggcctca tccagccggg ctgccatctg ggagggcatc
      601 cccggctccg gcttcaccag cattgccgtg atttccagcc cttccctgac ccgacgcacc
      661 gtacactggc ctacagggcg ggaatggtca tgctgccaga gaaacgggat cgtactgcca
      721 aactccgccc cctccggctc caggatgtca ccatcccgat ccggagaagg cgttgacgca
      781 atcccggtga tcacccgttc atcctcactg aaggatttca ccgtcagcag ggaacaggcc
      841 cgtttaagag tcacatcagc ctcctgaaaa taaaaaaacc gccgcagcgg ttcatgatgg
      901 ttacagggtg agcagggtta tatgaaaaaa acctcatacg ctttcttttt cggttccgga
      961 ttcagggaca tcagggacac cgcattgaa
//
LOCUS       DAZFWL020000441          976 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFWL020000441 DAZFWL020000000
VERSION     DAZFWL020000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.447"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..954
                     /locus_tag="ACHKNP_RS27645"
                     /old_locus_tag="pgaptmp_005529"
                     /pseudo
     CDS             1..954
                     /locus_tag="ACHKNP_RS27645"
                     /old_locus_tag="pgaptmp_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          286 a          227 c          254 g          209 t
ORIGIN      
        1 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
       61 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      121 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      181 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      241 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      301 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      361 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      421 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      481 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      541 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      601 aaagctctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      661 gatcgaggtt cacagtactg cgcatacgat taccgggtca tacaggagca gtttggtctg
      721 aaaacatcaa tgtcgcgtaa aggtaactgt tacgacaacg ctccgatgga aagcttctgg
      781 ggaacgctga aaaatgagag cctgagccac tatcgtttta ataaccggga tgaagccatc
      841 tcagtaatac gggaatacat tgagattttc tacaatcgtc agcgtcgtca ctctcgtctg
      901 gggaatatct ccccggcagc cttcagggaa aaatatcatc agatggctgc ttaaaaaaag
      961 aacaaatggt agtgtc
//
LOCUS       DAZFWL020000442          973 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFWL020000442 DAZFWL020000000
VERSION     DAZFWL020000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.448"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..314
                     /locus_tag="ACHKNP_RS27650"
                     /old_locus_tag="pgaptmp_005530"
     CDS             <1..314
                     /locus_tag="ACHKNP_RS27650"
                     /old_locus_tag="pgaptmp_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HGU4707453.1"
                     /translation="WAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSYRKD
                     KRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDKLKR
                     S"
     gene            342..973
                     /locus_tag="ACHKNP_RS27655"
                     /old_locus_tag="pgaptmp_005531"
                     /pseudo
     CDS             342..973
                     /locus_tag="ACHKNP_RS27655"
                     /old_locus_tag="pgaptmp_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          271 a          203 c          255 g          244 t
ORIGIN      
        1 actgggccgc atttgcaata aatatcaata aatatgataa tagtgatatt agggattgtt
       61 ttgtggagat gcttcaggat aaaaatgaag aagtccgtat tgaagcaata atcggacttt
      121 cctacagaaa agataaaagg gttttatctg ttttatgcga tgagttaaaa aaaaatactg
      181 tttatgatga tatcattgaa gctgcgggtg aattaggtga taaaacgcta cttcctgttt
      241 tagatactat gttgtacaag tttgatgaca atgaaattat aacttccgct attgataagc
      301 tgaagcgttc atagcgtgag ttgcctatgc acagtggggg attcccgccg gcacggtgca
      361 gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg gtgagtgagg aaagccgccg
      421 ctggctggca tcgtgcggcc tgaccgtgga gcagatgaaa aaccagatgg acccggtgta
      481 cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat cgcggcctgc cgctggcgct
      541 catcagcacg gaaggggcaa cagcgtggtg cgcagaatat gatgaatggg gcaacctgct
      601 gagtgatgag aacccgcatc atctgcagca gctcattcgc ctgccgggtc agcagtatga
      661 tgaggagtcc ggcctgtatt acaaccgcca ccgctattat gacccgctgc aggggcggta
      721 tatcactcag gatccgattg ggctgaaggg gggatggaat ttttatcagt atccgttgaa
      781 tccggtcata aatgtagatc cgcaaggttt ggttgatata aatttatacc ccgaaagtga
      841 tcttatccat tctgtagctg atgagattaa tatcccaggc gttttcacaa tcggggggca
      901 tggtaccccc acatctattg aatccgcaac gcgcagtatc atgacagcta aagatctagc
      961 atatctaatt aaa
//
LOCUS       DAZFWL020000443          967 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFWL020000443 DAZFWL020000000
VERSION     DAZFWL020000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.449"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..208)
                     /gene="tnpA"
                     /locus_tag="ACHKNP_RS27660"
                     /old_locus_tag="pgaptmp_005532"
                     /pseudo
     CDS             complement(1..208)
                     /gene="tnpA"
                     /locus_tag="ACHKNP_RS27660"
                     /old_locus_tag="pgaptmp_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            216..>967
                     /locus_tag="ACHKNP_RS27665"
                     /old_locus_tag="pgaptmp_005533"
     CDS             216..>967
                     /locus_tag="ACHKNP_RS27665"
                     /old_locus_tag="pgaptmp_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU4707454.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYL"
BASE COUNT          276 a          231 c          235 g          225 t
ORIGIN      
        1 atggacgtga tctggttcgc catccatttc aaccagttca gcttcaaaat aagcacatac
       61 atttgaaaag taagtgcgta gtttttctgt cgcgtcaaaa aatctggcgt cggtatctgg
      121 tgacaaagac caggtgaaca tgcatcagga aaacacaatg ccttccacgc cggatatcgg
      181 tttctttatt cacagaccaa agtatcattt gacctgtgaa acgattacag gcatttaaat
      241 tccagttaag accaggtggt caacaggagt gtgaaatgag gcgcttcgca ggcgcatgtc
      301 gtttcgtttt caatcgtgct ctggcacgtc agaatgagaa tcatgaggcc ggtaataaat
      361 acatccctta cgggaaaatg gcttcctggc tggttgagtg gaaaaatgcc actgaaacgc
      421 aatggcttaa agattctccc tcacagccat tgcaacagtc actgaaagac cttgagcggg
      481 cttacaaaaa cttcttccgg aagcgggctg cttttccccg attcaaaaag cggggacaga
      541 atgatgcatt ccgctacccg cagggcgtta agctcgatca ggaaaacagc cgtatttttc
      601 tgccgaaact gggctggatg cgctaccgga atagccgtca ggtcacgggt gttgtgaaaa
      661 atgtcactgt cagccagtcc tgcggtaagt ggtacatcag tattcagaca gaaagtgaag
      721 tatccactcc ggctcaccct tcagcatcaa tggtcgggct ggatgctggc gtggctaaac
      781 tcgccacgct gtcagatggc acagtctttg agcctgtaaa cagttttcag aaaaaccaga
      841 agacgctggc gagacttcag cgacagttaa gccgcaaggt caaattcagc aacaactggc
      901 agaagcagaa acgcaaaata cagcgactgc attcctgtat cgcaaatatc cgcagggact
      961 accttca
//
LOCUS       DAZFWL020000444          963 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFWL020000444 DAZFWL020000000
VERSION     DAZFWL020000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.450"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(40..636)
                     /gene="fimE"
                     /locus_tag="ACHKNP_RS27670"
                     /old_locus_tag="pgaptmp_005534"
     CDS             complement(40..636)
                     /gene="fimE"
                     /locus_tag="ACHKNP_RS27670"
                     /old_locus_tag="pgaptmp_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HGU4707455.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          308 a          202 c          174 g          279 t
ORIGIN      
        1 tatttatttg ttttttaact ttattatcaa tgagttaaat caaacctctt ctctttttaa
       61 tttttcgttt atgagattat ttctttccca taatccggca aaacgagcag cattactggc
      121 ggtataacgt acagtatggc gaatatttcg atgcccgaga taatcctgaa ttaaacgagt
      181 atctgcacca cgctccgcca gttcataacc gcaagcatgc cttaacatat gaggatgagt
      241 ctgcgttacg gttccagctt caataccggc atcgcgaata atgcgatagg cctgctggcg
      301 agaaagccga ctcccacggc gagaaataaa tatagcgtcg gtccggtcag cgcctttcca
      361 gttagcacgt tcctgggtcc agcgttccac ggcttcacgc tcatcaaaac gtaacgggtg
      421 aacggtagaa aatccgttct tcagtcggcg aatatttatt cgaccttcat taaggtcaag
      481 atcctgataa tgcagatcaa gcagttcact aatacgcatc ccatgccgat atgccaacag
      541 aataagacaa taatctctgg ctcccgttgc cccgtaacaa accgcctgca tcatggcctg
      601 aacttcttta ccggtaagat aacgacgttt actcacaata gtagtactcc tgactgagat
      661 atatttgaat gaatacctat aggaaacctc aatcggtcaa aattagccca atagataaaa
      721 agataaccac acacgaagtg atgtggctat cagtcaatta cattaaatat catacaaaaa
      781 taaaatatca ctgatgtgat aacatctatt ttcattgatt tgattcaata taacaataga
      841 ttgcagtgga ttaacttcca gttgcttaaa aaataaagaa aaaagtgcgg aaacaggaga
      901 atttaagtga tgtgtaattt tatgaatgat tttgtttatt tatttttaag aacataaacc
      961 att
//
LOCUS       DAZFWL020000445          960 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFWL020000445 DAZFWL020000000
VERSION     DAZFWL020000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.451"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            156..788
                     /locus_tag="ACHKNP_RS27675"
                     /old_locus_tag="pgaptmp_005535"
     CDS             156..788
                     /locus_tag="ACHKNP_RS27675"
                     /old_locus_tag="pgaptmp_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4707456.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQY"
BASE COUNT          325 a          164 c          178 g          293 t
ORIGIN      
        1 taacgagaaa tgcattttaa aatgtcttaa tcagaattat agcaaataca gattaatcgc
       61 aatttttccc agcaaaatta caaaatcatg gtgtattgac atcatcaatc accaatgaaa
      121 ttatgcaatt atatacggat agggaggttc ttaacatggg gctttgtagt cgttataaaa
      181 gtcttacatg caatagttgc tcaatgcatt gccaaataat gccagaagag tcaccgcgtt
      241 tgcagtactg cgcaaactcg tgtttttgta tgtggcccga agaaagttca tattttaatc
      301 gtggtgtggt agaaggtatc ttaacaaaaa accacaatgc gagattaagc ggatatattt
      361 ttgtcgattt ttcagtaagt tttttacgtc tattccttga aaaagactgg attgactatc
      421 ttgcaagtac tgacatgggg attgttttag tcagcgaccg caatatgcaa tcgttagcta
      481 attactggcg aaaacacaac tcagcaatat ctgctgtcat atataatgat gatggtcttg
      541 atgttgccaa tgaaaagatc agacaactat ttattggtcg ttatttatca tttaccagag
      601 gtaacacatt aactcagatg gaattcacca taatggggta tatggtttct ggttataatc
      661 catatcagat tgctgaagtt ctggatatgg atatccgcag catctacgcg tacaagcaac
      721 gaatcgaaaa gagaatgggt ggtaaaataa acgaattatt tattcgttca cattcggtcc
      781 aatattgata ctcaacgacc agccagaatc atactctgtt tatacgggca tttattgaaa
      841 tgtccgtatt ttataaatag gccgtcccct caagagtaaa caccatagta tttaaaacca
      901 tcacacgaaa aattcagaag cattacgaaa tatgaattta caaacaatag tggcataaat
//
LOCUS       DAZFWL020000446          959 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFWL020000446 DAZFWL020000000
VERSION     DAZFWL020000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.452"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>959
                     /locus_tag="ACHKNP_RS27680"
                     /old_locus_tag="pgaptmp_005536"
     CDS             <1..>959
                     /locus_tag="ACHKNP_RS27680"
                     /old_locus_tag="pgaptmp_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HGU4707457.1"
                     /translation="IKKILTEISKMPEDSIFTKINTRIPRDTMPILRRYHLWPDGWNI
                     RGLNGFMLSHKGSEVIDAVIAGQNQAYRELRRIRDNIHSEIYFKQTDELSSLPDTDKI
                     GGILVKKYLSGSLFSKFRQDTIIPEALSTLQISGPDLIQRKMLQFFRSRGVLGEEFIN
                     ERKLSDKAYIGVYKTTGTGKYDWLNPESIGVNDVTPADESTWCIGKGRCVDDFLFKDV
                     SKLKTENLPELFLTKIDTDTFFSQWSTKTKKDLQKKIQDLTVRYNELIDSSTIDFKNL
                     YEIDQMLHMIMLEMNDDIAKRSLFSLQVQIAEKIRRMTIPVDN"
BASE COUNT          346 a          163 c          189 g          261 t
ORIGIN      
        1 tatcaagaag atattaacag aaatatctaa aatgccagaa gatagcattt ttactaagat
       61 caatacaagg attcctcgag acacaatgcc catccttcgt cgttatcacc tatggcctga
      121 tggatggaat attcgtgggc tcaatggatt catgctatca cataaaggta gtgaagtgat
      181 tgatgctgtt atcgcaggcc agaatcaggc ttacagggaa ctaagaagaa taagagataa
      241 tattcatagt gaaatatact tcaaacaaac tgatgaattg tcctcacttc cagatacaga
      301 caaaattgga gggattctgg taaaaaaata cctttcagga agtctttttt caaaattcag
      361 acaagatact attatcccgg aggcattgag caccctccag atatcaggtc ctgacttgat
      421 tcaaagaaag atgttgcaat ttttcaggag tagaggggtg ttaggtgaag agttcattaa
      481 tgaaagaaaa ctgagtgata aagcttatat tggtgtctac aaaacaactg gcacagggaa
      541 atatgactgg ttaaaccctg aatcgatcgg cgttaatgat gtcacgcccg cagatgaaag
      601 tacctggtgt ataggaaaag gccggtgtgt tgatgacttc ctgttcaaag atgtttcaaa
      661 actaaaaaca gaaaatcttc cagaattatt cttaacaaaa atagatactg atacgttttt
      721 ctctcagtgg tcaaccaaaa ccaagaaaga tctgcaaaaa aaaatacaag accttactgt
      781 acgttataat gagctaattg actcgtcgac tatcgacttt aaaaatctat atgaaataga
      841 tcaaatgctc catatgatta tgctagagat gaatgatgat atagccaaaa ggtcattgtt
      901 ttcattgcaa gttcaaatag ccgaaaaaat tcggaggatg accattcctg tagacaata
//
LOCUS       DAZFWL020000447          958 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFWL020000447 DAZFWL020000000
VERSION     DAZFWL020000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.453"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>958)
                     /locus_tag="ACHKNP_RS27685"
                     /old_locus_tag="pgaptmp_005537"
     CDS             complement(<1..>958)
                     /locus_tag="ACHKNP_RS27685"
                     /old_locus_tag="pgaptmp_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU4707458.1"
                     /translation="GEFFDIIHNALISPGIKLIVGPRGCGKTHLMRYAWVECKNDDNA
                     PLPLYVSFNKYFKLEPLLKNKPNAIDLFHKWVLAKILLSAYEIACDIEDSEYLDLSTI
                     LIADKEFLINLIVRLEQGLSPSLEQENIVQLISVSSVISSLSSLCDWLERKRVIILLD
                     DAAISLTPEYLYEFFDIVRSLKTSKIALKASVYPGTTEYGPRFHVAHDAETIDAWISV
                     QHPNYSSVMDAIAQARFPGYDGIPDDLKELFKFSAFGIPRSFLMMLRDYQTTLSQTTQ
                     QKFNKIIERYVELRLSEYSSLKDKLPRLFDIVSLGESLLLKVV"
BASE COUNT          323 a          179 c          157 g          299 t
ORIGIN      
        1 cgactacttt taataaaagt gattcaccta atgaaacaat gtcaaacaat cgaggtagct
       61 tatcttttag tgaactgtat tcgctgagtc taagctcaac atacctttca attattttat
      121 taaatttttg ctgagttgtc tgacttaaag ttgtttgata atctcttaac atcatcaaaa
      181 aagaacgtgg aataccaaat gctgagaatt taaataactc tttcaagtca tccggaatgc
      241 catcataccc aggaaacctt gcctgcgcta tagcatccat cactgaggaa taattagggt
      301 gctgtaccga aatccaagca tcaattgttt cagcatcatg agcaacatga aaccttgggc
      361 catattcggt tgttccagga taaaccgatg cctttaaagc tatttttgag gtttttagac
      421 tacgtacaat atcaaaaaac tcgtaaagat attcaggagt taagcttatt gctgcatcgt
      481 ctaataaaat aatgactctt ttacgttcaa gccaatcaca tagtgaactc aacgaggata
      541 tgacagaaga aacactgatc aattgaacaa tgttttcttg ttctaatgat ggcgaaagcc
      601 cctgctcaag ccttacgatt aaattaatta ggaactcttt atccgctatg agtatcgttg
      661 acaaatcaag atattcagaa tcttcaatat cgcaagcaat ttcataagca gagagtagta
      721 tctttgctaa tacccattta tgaaagagat caattgcatt tggtttattt tttaaaagcg
      781 gctctaactt aaaatattta ttaaaagaaa catagagtgg taatggtgcg ttatcatcgt
      841 ttttacattc aacccaagca taacgcatca aatgtgtttt accacatccc ctaggaccaa
      901 ctataagttt gatgcctggg gatataagag cattatgtat aatatcgaaa aactctcc
//
LOCUS       DAZFWL020000448          953 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFWL020000448 DAZFWL020000000
VERSION     DAZFWL020000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.454"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            69..293
                     /locus_tag="ACHKNP_RS27690"
                     /old_locus_tag="pgaptmp_005538"
     CDS             69..293
                     /locus_tag="ACHKNP_RS27690"
                     /old_locus_tag="pgaptmp_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707459.1"
                     /translation="MKKLQVTVKPFQGTIPFRVLQHGRVLLEEVFRGKCTECYSRTYE
                     VNATHEEFTVECVMNTDKCRMVSAELQPVC"
     gene            complement(662..889)
                     /locus_tag="ACHKNP_RS27695"
                     /old_locus_tag="pgaptmp_005539"
     CDS             complement(662..889)
                     /locus_tag="ACHKNP_RS27695"
                     /old_locus_tag="pgaptmp_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4707460.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
BASE COUNT          249 a          184 c          233 g          287 t
ORIGIN      
        1 gtggaagggg cacaggacta tatccgcact cagtgtacta agcaggcttt ttattatccg
       61 gaggatgtat gaagaaatta caggtaacgg taaaaccttt tcagggaaca attccgttcc
      121 gtgttttgca acatggccgt gttctgcttg aagaggtgtt cagaggtaaa tgcactgaat
      181 gttattcacg aacatatgaa gtgaatgcca cgcatgaaga attcaccgtt gagtgtgtga
      241 tgaatactga taaatgccga atggtatccg ctgaattaca gccagtgtgt tgagcgacct
      301 tattatccat gcgcggtatt gtcgccgtat tcctgcatta acagagaccg cagcccgaca
      361 gggagactcc tctgcgagag tgtgcgggga taatcaaaaa cgatacacac cggggtttac
      421 cgcgtaaacg gagcgcggcg ttctcccctc atggtcgccc gtccggtgcg atggtggaag
      481 aaactggaat ctgttcaata aaaaaactgc cgtgttggag tcacagcagt aatgtactga
      541 ttgggtagaa gattattatt gttatgcttt atttttattc tatatggctg attatttcaa
      601 ttcggaatta atacagctaa tgtctgtgat tttttataaa ttcagcaata taaagaaata
      661 gttatatgaa cagccatcgc agagcatact gtgtatcatt cttttttata gtcaactgac
      721 gggcatattt tatgtctgct gccagctccc ggcggcaaga ttcaatgacc cacgcagaaa
      781 aattttctga acctttctgg tcaagagcga tgttaatttg ttcaatcatc tggtttggaa
      841 atcggatgtt gcgggttgtt gttctgcggg gccggttttt cgatgacatt ttctttcctc
      901 tggtgacaag ctatatggcg aggattttac atggctgtgc ttcgtacgtt acc
//
LOCUS       DAZFWL020000449          953 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFWL020000449 DAZFWL020000000
VERSION     DAZFWL020000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.455"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>953
                     /locus_tag="ACHKNP_RS27700"
                     /old_locus_tag="pgaptmp_005540"
     CDS             <1..>953
                     /locus_tag="ACHKNP_RS27700"
                     /old_locus_tag="pgaptmp_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TyeA family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HGU4707461.1"
                     /translation="NSNSLDFELESQQLTQKNSSNSYSPLLNLQNELAMITSSSLSET
                     IEGLSLGYRKGSARKEEEGTTTEKLLNDMQELLTLTDSDKLKELSLKNSGLLEQHDPN
                     LAMFGNMPKGEIVALISSLLQSKFVKIELKKKYAKLLLDLLGEDDWELALLSWLGVGE
                     LNQEGIQKIKKLYEKAKDEDSEKGASLLDWFMEIKDLPEREKHLKVIIRALSFDLSYM
                     SSFEDKVKTSSIISDLCRVIIFLSLDNYTDIIAISINKDKDVILNEVLSIIEHVWLTE
                     DWLLESPSRVSIVEDKHVYYFHLLKDFFASLPDACFIDREQ"
BASE COUNT          309 a          136 c          199 g          309 t
ORIGIN      
        1 ttaattctaa ttcattagat tttgaattgg aatctcaaca attaacgcaa aaaaattctt
       61 ctaatagtta ttctccatta ttaaatttac aaaatgaatt agcgatgatt actagttcat
      121 cgctttctga aacgattgaa gggctaagcc tgggatatcg caaaggcagt gcaaggaaag
      181 aagaggaagg tacgacaacg gagaagctgc ttaatgatat gcaagagctg cttactctga
      241 ccgatagcga taaattaaaa gaactatcat taaagaacag tgggctgctg gaacagcatg
      301 atcctaattt ggctatgttt ggtaatatgc caaaagggga gattgttgca cttatatctt
      361 ctttattaca atctaagttt gttaagattg aattaaaaaa gaaatacgcc aaattattat
      421 tagatttatt aggcgaagat gattgggagt tggctctgct ttcctggtta ggggtgggtg
      481 agttaaatca ggaaggtatc cagaagatca aaaagcttta tgaaaaggcc aaggatgagg
      541 attctgaaaa gggagcctct ttacttgact ggtttatgga gattaaggat cttccagagc
      601 gtgagaagca cttaaaggtt attattaggg cgctatcgtt cgatctgtct tatatgtctt
      661 cttttgaaga taaggtgaaa acatcttcaa ttattagcga tttatgccgg gtaatcattt
      721 ttttatcgct tgataactat acagatatta ttgcaatctc tattaataaa gataaagatg
      781 tcattttaaa tgaagtattg tctattattg aacatgtatg gttaacagaa gactggctcc
      841 tggaaagccc ttctcgggta tcgattgtcg aagataaaca tgtttattat ttccacctat
      901 tgaaagactt ttttgcatca ttacctgacg cttgttttat tgatagggaa cag
//
LOCUS       DAZFWL020000450          949 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFWL020000450 DAZFWL020000000
VERSION     DAZFWL020000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.456"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>949)
                     /locus_tag="ACHKNP_RS27705"
                     /old_locus_tag="pgaptmp_005541"
     CDS             complement(<1..>949)
                     /locus_tag="ACHKNP_RS27705"
                     /old_locus_tag="pgaptmp_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HGU4707462.1"
                     /translation="DNVEIGGIPAKKYLEHGLFSEYRQDGTIPYVVSTLNISGPDMIM
                     RQMKKYYKSLGRIGEVHIKDNKLSDMNFMGVYASSDKENKSFNWLNPVSVGVNDITPD
                     DESSWAVRNNDINKILFEKINCHVPEKLPTSLYYEIDSRVFFHGWDNKSIQYVTEINK
                     DLIKDINLLLTSSNVDVKLLIKLDRELYAISSKIENPLALRSIRTLQLQLTNYVTSNT
                     FEPENTINFIYDFYSKKQNDLLSAIKLFSRNDVETKIIVWYNSTMEKNVFLREVISCV
                     LWTKKVDSYIKENKKHLSTEDAEALRDYAKLKIKELFSML"
BASE COUNT          292 a          161 c          126 g          370 t
ORIGIN      
        1 ccaacatcga aaatagttct tttattttta attttgcata atctcttaaa gcttcagcat
       61 cttccgtaga taagtgtttt ttgttttctt ttatataaga gtctactttt ttagtccata
      121 gaacacatga tataacctct cggaggaata catttttttc cattgttgaa ttgtaccaaa
      181 caatgatttt tgtctcgaca tcattacgcg aaaataactt aattgcgcta agtaaatcat
      241 tttgtttttt actataaaaa tcataaataa aattaattgt attttcaggt tcaaatgtgt
      301 ttgaggttac ataattagtt aattgcagct gtaaagtcct tatagagcgc aaggctaacg
      361 gattctctat tttgctgctt attgcatata attcacgatc tagctttatt aaaagtttaa
      421 catcaacatt agacgatgtt aacaataaat ttatatcttt aataaggtct ttattaatct
      481 cagtaacata ttgtatgctt ttattatccc atccatgaaa aaaaactcgc gagtcgattt
      541 catagtatag ggaggtaggt agtttttcgg gtacatgaca gtttattttt tcgaataaaa
      601 ttttattaat atcattattt ctaacagccc atgagctttc atcatccggt gtaatatcat
      661 taacaccaac actcactgga ttcagccagt taaaactttt attttcttta tctgaacttg
      721 catatacccc cataaaattc atatcactta atttattgtc tttaatatgt acttcaccta
      781 tgcgccctag agatttatag tattttttca tttgtcgcat aatcatgtct gggccagata
      841 tatttagagt acttactaca taaggaattg taccgtcctg tcgatattca gaaaacaaac
      901 cgtgttctaa atattttttt gcaggtattc ctcctatttc cacgttatc
//
LOCUS       DAZFWL020000451          948 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFWL020000451 DAZFWL020000000
VERSION     DAZFWL020000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.457"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>948
                     /locus_tag="ACHKNP_RS27710"
                     /old_locus_tag="pgaptmp_005542"
     CDS             <1..>948
                     /locus_tag="ACHKNP_RS27710"
                     /old_locus_tag="pgaptmp_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HGU4707463.1"
                     /translation="DFLINDERVFNLEGLIKKYKYLSLSEIHKTLTNSNSFADISIPL
                     LQTICPSITTIIKKTEYHGHQLTNAMTVASVVKPYDFSNLGAINSIDKSVSDIPALHT
                     IVEQAKYNLLSWNDFYNTHASIWDTIARQHKSTNIEFHPQSLLFDRDSKGKCLGLSLL
                     YLDTGGYGSRYQKLRHNIETASALYQTKYNDNIKLSNSDDFFLRKTQRIITMSNELGN
                     NRLKNSQLEILELKDPVLTEGILYQRRISSLLITTEYHSLALQQVSSFWRVTDPNFGH
                     CDFHSLAQALTFIKNITSNGNFSSLYGSGIIKIYFSESP"
BASE COUNT          349 a          149 c          146 g          304 t
ORIGIN      
        1 tggatttttt aattaatgac gagagagttt ttaatcttga aggcttaata aaaaaatata
       61 aatatttatc attgagtgaa atacataaaa ctttaacaaa tagtaattca tttgcagata
      121 tttccatacc attattacag actatatgtc ccagtataac aacaataata aaaaaaacag
      181 aatatcatgg acatcagtta acaaatgcta tgacagttgc ttcagtggtt aagccatatg
      241 actttagtaa tcttggtgcc ataaatagta tagataagtc cgtttctgat atacctgcat
      301 tgcatacaat tgttgaacaa gcgaagtata atttactttc atggaatgat ttttataata
      361 ctcatgcgtc aatatgggat acaatagcaa ggcagcataa aagtacaaat atcgaatttc
      421 acccacaaag tcttcttttt gatagagatt cgaaaggaaa gtgccttggt ttgtctctcc
      481 tgtatcttga tacaggaggt tatggaagca gatatcaaaa actgagacac aatattgaaa
      541 ctgccagcgc attgtatcaa acaaaatata atgacaatat taaattatct aatagcgatg
      601 atttcttttt aagaaaaaca caacgtatca ttacaatgtc caatgagtta ggcaataata
      661 gattaaaaaa ctcacagtta gaaatcctag aattgaagga ccctgtacta actgaaggta
      721 tattatacca gcgtcgaata tcatctcttt taataacaac agaatatcac tcactcgcgt
      781 tacaacaagt atcttctttt tggagagtaa ccgatcctaa ttttgggcat tgtgacttcc
      841 attccttagc acaggcttta acgtttataa aaaatataac gagcaatggt aacttttcat
      901 ctttatatgg aagtggtatt attaaaattt actttagtga gtctccta
//
LOCUS       DAZFWL020000452          946 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFWL020000452 DAZFWL020000000
VERSION     DAZFWL020000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.458"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(397..945)
                     /gene="hdeD"
                     /locus_tag="ACHKNP_RS27715"
                     /old_locus_tag="pgaptmp_005543"
     CDS             complement(397..945)
                     /gene="hdeD"
                     /locus_tag="ACHKNP_RS27715"
                     /old_locus_tag="pgaptmp_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013368005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HGU4707464.1"
                     /translation="MLKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDILSTV
                     VGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIAAF
                     IAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVSTLV
                     GIELIFSAASLFSFASLFVKQQ"
BASE COUNT          321 a          219 c          193 g          213 t
ORIGIN      
        1 taaataacgc attcatgtct tcaggttttt atatagcgtc tggagacacg gatatttatg
       61 caatgtaatc aacgccagtt gcaaaaaaat gaacccggat tacttattta accgcccata
      121 gcatcaacgc ttacggaact ctttgttcac tttcccacag cacttttaaa acattaaacc
      181 tgactacgga aaatatcagc catgataatg tttgaaatgg ctaatttgcc atggagtgaa
      241 aaaaattaga tgaaattcag taggttgaaa tgatcactag caggtaatta tttcaatgat
      301 agtgcgcaat tgatctacaa cactgcgtag cggagagagt attaatcgga tcatagtcac
      361 atcaagtgac tatgatcctg gtgacaaccg ggataattat tgctgcttaa cgaacaaact
      421 ggcgaagctg aacaggctgg cggcgctaaa tatcagttca attcccacca gtgtggaaac
      481 cagcgttaca gacaccatcg gcgttgcacc aaggaatatc caggcaatga cgatatccag
      541 cacaccaata acgagctgta gccagctgcc tttcattgaa cgctgacgat accaactcat
      601 caggcgaata acccctgcaa cacagaacaa accggcaata aatgccgcaa tggcaaaaat
      661 gcccagctcc ggtgcgcgga tgaagaaata gccgatcaat aaataggcga ctgcgacgag
      721 gaaaccggat aataccggcc agaaattatg actgcggttg ctgaataacc cgacaataag
      781 cgcaataccc gagcagatta ataatgcacc cactactgtg cttaaaatat cgccagagac
      841 gaacgggaaa ctgatacaca gcaacccgac gataaacagc agcacggcaa taaactggat
      901 tgctctgcga tgctttttca gcatctccag atcaaacttc aaaatt
//
LOCUS       DAZFWL020000453          937 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFWL020000453 DAZFWL020000000
VERSION     DAZFWL020000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.459"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>937
                     /locus_tag="ACHKNP_RS27720"
                     /old_locus_tag="pgaptmp_005544"
     CDS             <1..>937
                     /locus_tag="ACHKNP_RS27720"
                     /old_locus_tag="pgaptmp_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HGU4707465.1"
                     /translation="GRRKAIRTFATALTNIEKYPDYIFGASQYQLFHWIKKDYPYFFE
                     KLKKQIAAGRFELQGCFWVECDLNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDV
                     FGFPATLPQILKKSGINYIASQKLSQNKINKFNNYLFRWQGLDGSEILMHNFPEDTYD
                     SRARARSLEYIEQNYNEKEICPYAFMVYGVGDGGAGPGEEHIERLTRIRNIDGLPHVD
                     FSRVDKFFTHADAFRESLPIISGELYFEAHQGCFTSESATKAHNRIMENKLHDAEFFI
                     TITNNMTSILRSEFDEIWKAMLTLQFHDILPGSCIS"
BASE COUNT          289 a          199 c          182 g          267 t
ORIGIN      
        1 ggacgcagaa aagcaataag aacatttgcc accgccctga caaacattga aaaatatcct
       61 gattacattt ttggcgctag tcagtaccaa ctttttcact ggataaaaaa agattatccc
      121 tatttcttcg aaaagttaaa aaaacaaatc gccgcaggcc gctttgagtt acaaggttgc
      181 ttttgggtcg aatgcgattt aaatctggta agtggtgaat cgctgataag gcaaatcatg
      241 catgggacac gattcacact gcagaatttt tcaaagaaga taaattatgt ctggcaaccg
      301 gatgtctttg gattccccgc cacgcttcca caaattctca aaaaatcagg cattaactat
      361 attgcatcgc aaaagctctc acaaaacaag attaataaat ttaataatta ccttttccgc
      421 tggcaaggcc tggatggctc agaaattctg atgcataact ttcctgaaga tacgtatgat
      481 agccgagccc gcgctcgctc tttagaatat attgaacaaa actataatga aaaagaaatc
      541 tgtccctatg cgtttatggt gtatggtgtc ggtgatggcg gcgctggccc cggtgaggaa
      601 catatagaac gattaacacg catacgcaat attgatggct tacctcatgt tgatttcagc
      661 cgtgttgata agttcttcac tcacgccgac gcctttcgcg aatccttgcc cattatttcg
      721 ggtgaacttt acttcgaggc tcatcaaggg tgctttacat cagagtcagc aactaaagcc
      781 cataatcgaa ttatggaaaa taaactccat gatgctgaat tttttataac tattactaat
      841 aatatgacgt caattcttcg cagtgagttt gatgaaatat ggaaagcgat gctgaccttg
      901 caattccatg acatccttcc cggctcatgt atttcaa
//
LOCUS       DAZFWL020000454          935 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFWL020000454 DAZFWL020000000
VERSION     DAZFWL020000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.460"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..224
                     /locus_tag="ACHKNP_RS27725"
                     /old_locus_tag="pgaptmp_005545"
     CDS             <1..224
                     /locus_tag="ACHKNP_RS27725"
                     /old_locus_tag="pgaptmp_005545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707466.1"
                     /translation="QRYDDSGVICTGGPGTLSGIATTKNSKSKGNGAGGVGGNRLIFR
                     YYRPVSAEGGSYLSGLRIPLDSITDWRNT"
     gene            complement(337..533)
                     /locus_tag="ACHKNP_RS27730"
                     /old_locus_tag="pgaptmp_005546"
                     /pseudo
     CDS             complement(337..533)
                     /locus_tag="ACHKNP_RS27730"
                     /old_locus_tag="pgaptmp_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            709..>935
                     /locus_tag="ACHKNP_RS27735"
                     /old_locus_tag="pgaptmp_005547"
     CDS             709..>935
                     /locus_tag="ACHKNP_RS27735"
                     /old_locus_tag="pgaptmp_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4707467.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPT"
BASE COUNT          247 a          249 c          234 g          205 t
ORIGIN      
        1 agcagcgcta cgatgactcc ggtgttatct gcaccggagg accgggaaca ttaagcggta
       61 tagcgacaac aaagaattcg aaatcaaaag ggaatggtgc cggaggcgtg ggcggtaata
      121 ggctgatttt caggtattac cggcctgtat ctgctgaagg gggctcgtat ttgtcggggc
      181 tgcgtattcc gctcgattcg atcacggatt ggcggaatac ctaactgtga ttgattcagg
      241 taccattttg cagggatccg gtgcgttact gtctgacaca acaaacgcag cgggtcgctc
      301 tgctccgccg attcctgata atccctcccc agtaacctac tgcaacagta caaatacact
      361 gacgcctgaa acggtaacca gaatcagcac cagcagtaac tcagtaaaca gagcagacga
      421 tcggtcttcc ggtaataacc ggaaggaacg acagcgccac aatacaggca acacactgta
      481 ttatgagtaa tcgaattacc gcaatccccg catttctttt tgcgtatcac gatagctccc
      541 tcgccacgcc ttatccgtaa ccggtttttt acattaaaaa aaacaacgcc gggaattcaa
      601 tcgtcagtcc ggagacgacc gttcgggtta tcacagagag cctgagcagg ggggctgcgc
      661 attttttacg atgtggctat tccgtatgaa ccatacggag atataaccat gacctacaaa
      721 tacaacccct tctggcagca acgtattcgt gagacggtgc ggcacgcact gaatgttcat
      781 ccccgcctga cggcattgcg ggttgacctg cgtttcccgg atgtaccggc agcaacggac
      841 gcagctgtga tatcccgctt catcaatgcc ctgaaagccc gaatcgacgc ttaccagaaa
      901 cgtaagcatc gggaaggtaa acgcgtgcat cccac
//
LOCUS       DAZFWL020000455          932 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFWL020000455 DAZFWL020000000
VERSION     DAZFWL020000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.461"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>932)
                     /locus_tag="ACHKNP_RS27740"
                     /old_locus_tag="pgaptmp_005548"
     CDS             complement(<1..>932)
                     /locus_tag="ACHKNP_RS27740"
                     /old_locus_tag="pgaptmp_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4707468.1"
                     /translation="TRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNT
                     VGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSIT
                     DVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGA
                     GIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITS
                     GGNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKLSANQIEGDIV
                     KTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDR"
BASE COUNT          198 a          262 c          215 g          257 t
ORIGIN      
        1 tgccggtcaa acggctgatc gtcataaacc ctgacggtaa tggtccctga tggccaccgc
       61 tccggtgccc gggagtcccg cggaaaagcc ttacccactg ttttgactat atcgccttca
      121 atctggttgg ctgacagttt ccccttaatc tgacagttct cattaattgt gacattgttg
      181 agcgtcccgg cgttcgcatt cacactgcca ctgatatctg catttttagc ggtcagcttt
      241 ccgtccggtg tcagggaaaa tgccggagga ttaccgccgc tggtaatggt gggagccgtc
      301 aggcgtttca ggaacacgtc gttcatgaat atctgatcgc cctgaccaac aaacatcggc
      361 tttgtgttgc cattcgcagg attaatcatc gcaatcctgt ctgccgccag cagcacctga
      421 ctctgcattc ctgctggcgt attctcaata ccggcaccga tacccgcaat ataaaggcgt
      481 ccgtccttca tctgttgcag cttcacagcc cacatgctgt tcagattatt atttgtatca
      541 acctgaacct tctgtatctg ctggatcgct gcactctggt cttccagttt cttattgacg
      601 gtctgcgtga tttcattact gacatccgtg atggacgttc tgatttccgc caggtcaggc
      661 gcaagctgac cgttatcaat ctgagtccac aactcctgag ccagatgggt tttccctatc
      721 tctcctttga aaaaatccag atagccggat gcatcatcac tcggctggcc aacagcctcc
      781 acaaatgccg atttgccaac ggtgttcaca ctgcggatat aaaagtaata atcatggccc
      841 ggtttgatat tgatactggc ggctatccag tacagccccg tgccaaggta gcgggctgtg
      901 gtttcaacct gcctgatatc ggtaatccgc gt
//
LOCUS       DAZFWL020000456          931 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFWL020000456 DAZFWL020000000
VERSION     DAZFWL020000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.462"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..880
                     /locus_tag="ACHKNP_RS27745"
                     /old_locus_tag="pgaptmp_005549"
     CDS             <1..880
                     /locus_tag="ACHKNP_RS27745"
                     /old_locus_tag="pgaptmp_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707469.1"
                     /translation="GSVIARFTLCLTSTLLTMSPLLMLNGAVKTGSIVRTIGRGVSYV
                     DIPLALAILGDSWYKAYKHGSSDNPNSAQRFISQEAAFKTTQRVLTQGLSLMSSLSGA
                     IMRSLEKGTPPQMMSLFIVNILNLLFHQNPYEGASASANALKRSTYSHNPDILNTQAI
                     ALCVDLQHTKNITMPLFKTKDRISYAFNGQRTSPEDQKQILKEVINSCTQGERAILNT
                     SQSETCKHKIDEIYEKRFSETELNTLPNEMKNFLIFLNKTHEKDISRLSMGNEVNEKI
                     IAVIVKTLAYRESMLC"
BASE COUNT          350 a          171 c          154 g          256 t
ORIGIN      
        1 aggttcggtt atagcacgat ttactctatg tttaacaagt acattactaa ccatgtcacc
       61 actgttaatg cttaatggag cagttaaaac aggtagcata gtaagaacta taggtagggg
      121 agtgagttat gttgatatac cattggcctt agctatatta ggtgactcgt ggtataaagc
      181 ttataaacat ggttcttctg ataacccaaa ttctgctcag agatttatat cgcaagaagc
      241 ggcctttaaa accacccagc gggtattaac acaaggatta agtctgatgt cctctttatc
      301 gggagcaatc atgcgctctc ttgagaaagg cacaccacca caaatgatgt ctttattcat
      361 cgtaaacata ctaaatctat tatttcatca aaatccatat gaaggggcat cagcaagtgc
      421 taatgctttg aaaagatcaa cttactccca taatccggac atactaaata ctcaggcaat
      481 agctctttgt gttgacctcc agcatacaaa aaatataacc atgccacttt tcaaaacaaa
      541 agacaggata tcttacgcat tcaatggaca aagaacatcc ccagaagacc aaaaacaaat
      601 actgaaagaa gttataaact cctgtaccca aggagaaaga gccattttaa atacatcaca
      661 atcagaaaca tgcaaacata aaatcgatga gatttatgaa aaaagattct cagaaacaga
      721 actaaataca ttaccaaacg aaatgaaaaa tttcttgata tttttaaaca aaactcatga
      781 aaaagatatt tcgcgtttaa gcatgggtaa cgaagttaat gaaaaaataa ttgcagttat
      841 agtcaagacg ttagcatata gagagtctat gttgtgttag actttaactc tatattgata
      901 taacattagc caaatattca acagtgtatg c
//
LOCUS       DAZFWL020000457          931 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFWL020000457 DAZFWL020000000
VERSION     DAZFWL020000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.463"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..117
                     /gene="gpW"
                     /locus_tag="ACHKNP_RS27750"
                     /old_locus_tag="pgaptmp_005550"
     CDS             <1..117
                     /gene="gpW"
                     /locus_tag="ACHKNP_RS27750"
                     /old_locus_tag="pgaptmp_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HGU4707470.1"
                     /translation="RRIEYTAASLDELNRAINDAESVLGTTRRRRRPLGVRL"
     gene            114..>931
                     /locus_tag="ACHKNP_RS27755"
                     /old_locus_tag="pgaptmp_005551"
     CDS             114..>931
                     /locus_tag="ACHKNP_RS27755"
                     /old_locus_tag="pgaptmp_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4707471.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKA"
BASE COUNT          189 a          224 c          321 g          197 t
ORIGIN      
        1 cgcagaattg aatatacggc ggcttctctg gatgagctta accgggcgat caatgatgcg
       61 gagtcggtac tggggacaac ccggcgtcgc cgtcgtccgc tgggagtgag gttatgaaac
      121 gaacgcctgt cctgattgat gtgaacggcg ttccgcttcg tgagagtctc agctacaacg
      181 ggggcggtgc aggatttggc gggcaaatgg ctgagtggtt gccaccggcg cagagtgccg
      241 atgcggccct gctgcccgcg ttgcgtctgg ggaatgcccg ggcagatgat ctggtgcgca
      301 ataacggaat agcggctaat gcggtggctc tgcataagga tcacattgtc gggcatatgt
      361 ttctgatcag ctaccgtccg aactggcgct ggctggggat gcgggagacc gcagcaaaaa
      421 gctttgtcga tgaggtggag gcggcctggt cggaatacgc cgaagggatg tctggcgaga
      481 tcgacgtgga aggaaaacgc acgttcacgg aatttatccg tgaaggtgtg ggcgttcatg
      541 cgtttaacgg cgaaatcttt gtgcagccgg tctgggatac ggaaaccacg cagttattcc
      601 gtacgcgttt taaagccgtg agtccgaaac gggtggacac gccaggacac ggtatgggga
      661 accgttttct gcgggccggg gtggaggtcg atcgatatgg ccgtgccgtt gcgtaccata
      721 tctgtgagga tgattttccg ttctccggga gtggacgatg ggaacggatc ccgcgtgaac
      781 ttcccaccgg gcgtccggcc atgctgcata ttttcgagcc ggtggaggac gggcagaccc
      841 gtggggccaa ccagttttac agcgtcatgg aacggctgaa gatgctcgat tccctgcagg
      901 caacacagct tcagtcggcc atagtgaagg c
//
LOCUS       DAZFWL020000458          929 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFWL020000458 DAZFWL020000000
VERSION     DAZFWL020000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.464"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..532
                     /locus_tag="ACHKNP_RS27760"
                     /old_locus_tag="pgaptmp_005552"
     CDS             <1..532
                     /locus_tag="ACHKNP_RS27760"
                     /old_locus_tag="pgaptmp_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707472.1"
                     /translation="KSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAYKIRFPNGTVD
                     VFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITPVTAIKVTPTSGTVAKG
                     KTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMAITVTGVKAGKVSIPVISGNGQ
                     FATVAEVTVTEAGAAG"
     gene            546..>929
                     /locus_tag="ACHKNP_RS27765"
                     /old_locus_tag="pgaptmp_005553"
     CDS             546..>929
                     /locus_tag="ACHKNP_RS27765"
                     /old_locus_tag="pgaptmp_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000479108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4707473.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTESPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGAVHVDTDITEVAKNNA"
BASE COUNT          234 a          210 c          294 g          191 t
ORIGIN      
        1 gaagtctgca ggagatacca gttttacgct ggcctggaaa ccgggagaag aaggtcagaa
       61 agggcttata ggctggtttg aaagcgggga tgtgcgggcc tataaaatcc gtttcccgaa
      121 cggcacggtg gatgtgttcc gtggctgggt cagcagtatc ggtaaggccg taacggcgaa
      181 ggaagtgatc acccgtacgg tgaaagtgac caacgtgggt aaaccttctg tggcggaaga
      241 acgcagcgaa attacgccgg tcactgcgat taaggtgacg ccgacatctg gtacggtggc
      301 aaaagggaaa acaacaaccc tgacggtttc ttttgagccg gaaagtgcaa ccgacaaaac
      361 gttcagagcg gtttccgccg atccgtcaac gggaaccatt gctgtgaaag atatggcgat
      421 cactgtgacg ggggttaagg ctggaaaagt gagtatcccc gtgatttccg gtaatggtca
      481 gtttgccacg gtagctgaag tcaccgttac tgaagcgggc gctgcagggt aaacggaggt
      541 aatacatgtt tctgaaaacc gaacaatttg aatataacgg tgtgtccgtc acgctttccg
      601 agctgtctgc gctgcagcgt atcgagcatc ttgccctgct gaaacgacgg gcagaacagg
      661 ctgaagccag cggtaacctg caggtgagcg tggaagacct tgtcagaacc ggcgcgtttc
      721 tggtggcgat gtccctgtgg cataaccatc cacagaaaac ggagtcacca tcaatgaatg
      781 aggctgtgat gcagatcgaa caggaggtgc tcaccacctg gcctgctgat gccattgccc
      841 gggcggaaga cgtggtgttg cgtctgtccg ggatgagcgg ggctgttcat gtggatacgg
      901 atatcaccga agtggcgaaa aataacgcg
//
LOCUS       DAZFWL020000459          927 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFWL020000459 DAZFWL020000000
VERSION     DAZFWL020000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.465"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            236..865
                     /locus_tag="ACHKNP_RS27770"
                     /old_locus_tag="pgaptmp_005554"
     CDS             236..865
                     /locus_tag="ACHKNP_RS27770"
                     /old_locus_tag="pgaptmp_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HGU4707474.1"
                     /translation="MRVYASEMEKEYGTAVAQELVTGLLSGQDYIKRNPDTEAMAKAQ
                     SIMNTWGYHKSNASIGDAPLLFGGSVLGLTVKGMATNAAIGISVNSAAQLAGKDPFSY
                     VDAIIAGVTAAATTGKGIIVSTPINMSGAAIGSSIKGEDPTNSVIGTGLGSVFGGGIG
                     KVISGSMGSTVKEGTSDIIGNIGGSITGEAIGNVTKGTLDEVDKSIKNK"
BASE COUNT          303 a          150 c          228 g          246 t
ORIGIN      
        1 agttttactt taaaaataaa aaatagttgt tgagaataac tttctgggag cgacatcttc
       61 tgacaaactg gataaagcca ttgagaaaat caggaacggc gataagacat tagccactgc
      121 taatgagctg attaagctgg aaaatgcaga taaacgtagt gatgctctgg tatccaaatt
      181 taataaagat ccttcacaat taaacagcgc agaacgtgct gaactggcag cttacttgcg
      241 tgtatatgct tcagaaatgg aaaaggagta cggtacagcg gtggcacagg agcttgttac
      301 tgggttactg tcaggacagg actatataaa acgtaaccct gatactgaag caatggcaaa
      361 ggctcaaagc attatgaata cctgggggta tcataaatct aatgcaagta ttggtgatgc
      421 tcctttgttg tttggaggaa gcgtactggg gttaacagtg aaaggtatgg cgactaatgc
      481 agcaattggt atcagcgtaa actcagctgc tcagcttgct ggaaaagatc ctttcagtta
      541 tgtggatgcg ataattgcag gggtaacggc agcagcaaca acaggaaaag gaattatagt
      601 ttcaacccct atcaacatga gcggcgcggc aattggtagt agtattaaag gcgaggatcc
      661 gacaaattca gttattggga caggattagg ttcagtattt ggtggcggta tagggaaagt
      721 catctcgggg tcaatgggct ctactgtcaa agagggaaca tctgatatta ttggtaatat
      781 tgggggatca attactggtg aagctatcgg gaatgtaaca aaaggaacac tagatgaagt
      841 tgataaatcc attaaaaata aataagagtg tcaactttta cattcttatc atactaataa
      901 cgcgctgcat agcttgcatc tttttta
//
LOCUS       DAZFWL020000460          925 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFWL020000460 DAZFWL020000000
VERSION     DAZFWL020000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.466"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..925
                     /locus_tag="ACHKNP_RS27775"
                     /old_locus_tag="pgaptmp_005555"
                     /pseudo
     CDS             1..925
                     /locus_tag="ACHKNP_RS27775"
                     /old_locus_tag="pgaptmp_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          328 a          175 c          171 g          251 t
ORIGIN      
        1 taacatctct aaggatgaat gggaaaacct ccatgcatta aaagaaaagc gtttaaatgg
       61 aatgcctaaa acaacaccca aaagtgacca ggttattatg ttgcaaaatc aatacgttcg
      121 tgaaatgcga aaatatggcg tacgaggttt acgttatgat gcggcaaaac actcaaaaca
      181 tgaacaaata gaaagatcaa taaccccacc gcttaaaaat tataatgagc ggttacacaa
      241 tactaactta tttaacccaa aatatcataa aaaagccgtt atgaattaca tggaatatct
      301 ggtaacttgt cagttggatg aacaacaaat gtcatcactg ctttatgaaa gagatgattt
      361 aagcgccatt gatttttcat tgctcatgaa aacgataaaa gccttttcat ttggtggaga
      421 tcttcaaacc cttgcatcaa aaccgggttc cacaatctca agtatcccat cagaaagacg
      481 gatattgatt aacattaacc acgattttcc taacaatggt aatcttttca atgactttct
      541 atttaaccat caacaagatg aacaattagc aatggcatat atagccgctc tgccgttcag
      601 caggccttta gtttactggg atggccaagt attaaaatca acgactgaaa ttaaaaatta
      661 tgatgggtcc acgcgtgtcg gcggtgaggc gtagcttaat aaaggttgct ctacctatca
      721 gcagctctac aatgaattcc acgcattata tatagataaa gcaggaatat ggagcgcatt
      781 tgagggtgta tctgcaacta aaaacgttct ggcctttagt cgtggggatt ctgtgaacat
      841 taatcactct cctcatgatg gactagttat aataaataaa ggaaacgaag aagttgaagg
      901 tacctggcct aacaaattgc aacct
//
LOCUS       DAZFWL020000461          922 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFWL020000461 DAZFWL020000000
VERSION     DAZFWL020000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.467"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(202..895)
                     /locus_tag="ACHKNP_RS27780"
                     /old_locus_tag="pgaptmp_005556"
                     /pseudo
     CDS             complement(202..895)
                     /locus_tag="ACHKNP_RS27780"
                     /old_locus_tag="pgaptmp_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          253 a          188 c          210 g          271 t
ORIGIN      
        1 aactgtcgtt acagtttgta ttccccgggt aaatttgtct gcgtaaaaaa atgggctgag
       61 caaaaagaca ataaataatt aaaaatcaat acgatagaaa acaacagcat tattgttatt
      121 ttactttcac cttatcgctg tcgattctca ttatcattca ttggtaaaaa acagcaaaag
      181 aggccctggt aatgactcca gtcagtagta cagtgttttc gacaaatgaa ccaatggctt
      241 ttcgtgaatt tccagggagc gtgaaaagca aatcgttttc cgggctggtc gtttgatgtg
      301 agtacgaagt gtcagattgt tgcgctcaat gcgccgggta aatatcttgc cgacaagatg
      361 cttatcctgt ggcatttctc tggtataact gctccggttg tctctggtta tcatgcctgt
      421 ggagaatggc ttcaggagtt ccggcaactc acgacatgtt tcatcagtgc gaggaccaaa
      481 agtgtaagcc agcacacctc cagatttggt gttatacgca taccagtgcc actgctgacg
      541 agctttattt tcgacaaaac tccattgttc atcaagttcg cagataagcg tcacatcggc
      601 actggcgact ggagatgtgg ttactttgcg tggtgagcgt tttataaagt gagaatgacg
      661 gtgttgatat ccaccttcag cgttctggag ctatcgcgaa ccccgactcc gttatgaacc
      721 atttcaacaa tttgctcttt aatgcctggt tttcgggttt cgtagatgta atgcagttaa
      781 aatacacacg tttgcaggat tagcactgga aacgctcatg atctgaggtg ctgtgatcat
      841 aatggtacac tttataggaa ttacagcggg gagaatgtac tgtaactgtt gccatatggt
      901 ctccagatac caatagaata cc
//
LOCUS       DAZFWL020000462          920 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFWL020000462 DAZFWL020000000
VERSION     DAZFWL020000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.468"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            232..920
                     /locus_tag="ACHKNP_RS27785"
                     /old_locus_tag="pgaptmp_005557"
                     /pseudo
     CDS             232..920
                     /locus_tag="ACHKNP_RS27785"
                     /old_locus_tag="pgaptmp_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          347 a          171 c          141 g          261 t
ORIGIN      
        1 ctttttaaaa tgagcgcttt ttgatagttg ttaacgtttc cctttacgat actatcactg
       61 ccttttttta tacacaaata ttaccgggtg gaaatgaaag ggaatttaaa ggtaattata
      121 tcaggaagat ataacgcatt atatttattg tgtgtagaag aaaatccatt cctgttctaa
      181 ttatccaatt taaacatctt agcataaaac aaatgatgaa taaggaattt tatgtcaatt
      241 gactttaccc caggtataat aaatacatat cacggcgata tttataactg cacaacaaat
      301 accgataatg ctaaaacgcc agacacaccg aagtggcctt gcgataattg ggaagaacaa
      361 taacccatta attccacatt ctctggagaa ggatatatct ctgatcaata tgatttagcg
      421 caacaccaac ttcaacaaat taatgcgtgc cacacgaaca ctacatatac aaatgcagac
      481 tactcaaaag ttgtggctca acttgtaagt cttattacta acattgaaac gataagttcg
      541 acacaactta cgcaacagac acaatcaata cttaatcaga ttaataacat tagatatgaa
      601 aaaaacaaaa gtgctgaatg tcgaatcatt gttgtcgcta atcctaaacc cgacaaggca
      661 atcataacta aaattagcgt agaagaggga atacccataa cattcagtgt ccaaaccatg
      721 ttttcagata ctaattttat tgctgaacaa cgagctgact taccaactaa tataaaagat
      781 attcaaagcc tgtatcaaaa aatgacgaag ttatatattg agcacagtga aaataaaaac
      841 aggatgaaag tctttgccgg aaccaacttt attgatttca atatgacagg acagaacctg
      901 tccgggttcg ttctaacctt
//
LOCUS       DAZFWL020000463          916 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFWL020000463 DAZFWL020000000
VERSION     DAZFWL020000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.469"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..612
                     /locus_tag="ACHKNP_RS27790"
                     /old_locus_tag="pgaptmp_005558"
     CDS             <1..612
                     /locus_tag="ACHKNP_RS27790"
                     /old_locus_tag="pgaptmp_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 34 domain-containing
                     protein"
                     /protein_id="HGU4707475.1"
                     /translation="NQLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLRGRYI
                     TQDPIGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPFPLPIPWPKSPAQQQADDNAAKA
                     LTKWWNDTASQRIFDSLILNNPGLALDITMIASRGNVADTGITDRVNDIINDRFWSDG
                     KKTDRCDVLQELIDCGDISAKDAKSTQKAWNCRHSRQSNDKKR"
     gene            633..>916
                     /locus_tag="ACHKNP_RS27795"
                     /old_locus_tag="pgaptmp_005559"
     CDS             633..>916
                     /locus_tag="ACHKNP_RS27795"
                     /old_locus_tag="pgaptmp_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HGU4707476.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNI"
BASE COUNT          308 a          169 c          207 g          232 t
ORIGIN      
        1 aaccagctga atgaagagaa cccgcaccag ctgcagcagc tcatccgcct gccggggcag
       61 cagtatgatg aggagtccgg cctgtattat aaccgccacc gctattatga cccgctgcgg
      121 gggaggtata tcactcagga tccgattggg ctgaaggggg gatggaattt ttatcagtat
      181 ccgttgaatc cagttacgaa tacagatcct ctggggttag aagtttttcc tagaccattc
      241 cccttgccaa ttccatggcc caaaagccct gcacagcagc aagcagatga taatgctgca
      301 aaagcattga caaaatggtg gaacgataca gcatcacaaa gaatatttga ctctctaata
      361 ttgaataatc cgggactagc attagatata acaatgatag cttctcgtgg aaatgttgca
      421 gacacaggga taactgatcg tgtcaatgac ataataaatg acagattctg gagtgatggg
      481 aaaaaaaccg acagatgtga cgtacttcag gaactaattg attgtggtga tattagtgct
      541 aaagatgcaa aaagcacaca gaaagcctgg aattgtcgtc actccagaca gtcaaacgat
      601 aaaaaaagat agcccttgtg gaggttcctg caatgtcaaa tacataccag aaaagaaagg
      661 caagtaaaga gtatggttta tataatcaat gtaagaaact aaatgatgat gaattatttc
      721 gcttacttga tgatcacaat tccttgaaaa ggatttcatc tgccagagta ttacagttaa
      781 gaggtgggca agacgctgtt agattggcaa ttgagttctg ctctgataaa aattatatcc
      841 gtagagatat cggagcattt atactcgggc aaataaaaat ttgcaaaaaa tgcgaagata
      901 atgtttttaa tatttt
//
LOCUS       DAZFWL020000464          916 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFWL020000464 DAZFWL020000000
VERSION     DAZFWL020000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.470"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>916
                     /locus_tag="ACHKNP_RS27800"
                     /old_locus_tag="pgaptmp_005560"
     CDS             <1..>916
                     /locus_tag="ACHKNP_RS27800"
                     /old_locus_tag="pgaptmp_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4707477.1"
                     /translation="TVNLIKSARVGYTKMLLGVVGYFIEHKSRNSLLFQPTDSAAEDF
                     MKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLGGAAAKNYREKSV
                     DVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIKGTCQIEKAANES
                     AHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEHHGCVIHQSELDQ
                     SNGRWICENTGMWTRDGLTFFSARGDEIPPPRSITFHIWTAYSPFTTWVQIVYDWLDA
                     LKDPNGLKTFVNTTLGETWEEAVGEKLD"
BASE COUNT          210 a          230 c          260 g          216 t
ORIGIN      
        1 gcactgttaa tctgattaaa tctgcccgtg ttggctatac aaagatgttg ctgggggtgg
       61 tcgggtattt tattgagcat aaatcccgaa acagtctgct ttttcagccc acggattctg
      121 ccgctgaaga ttttatgaag tctcacgtgg aggcgacgat tcggaacgtg ccatgcctga
      181 aagacctttc cccatggctg ggtcgtaaac atcgtgacaa tactctcacg ctgaaacgct
      241 tttcatcggg cgtcggtttc tggtgcctgg gcggcgctgc cgccaaaaac taccgtgaaa
      301 aatccgtgga cgtggtctgc tatgacgaac tttcctcgtt cgagccggat gtcgaaaaag
      361 agggctcgcc aaccctgctg ggggataagc gtattgaggg gtcggtgtgg ccaaaatcca
      421 ttcgcggctc gacgcctaaa atcaaaggca cctgccagat cgaaaaagcc gctaacgagt
      481 cggcgcattt tatgcgtttt tatgtgccct gcccgcactg tggggaggag cagtatctga
      541 aatttggcga tgagtccacg ccttttgggc ttaaatggga gaaggacagc cctgaaagtg
      601 ttttctacct ctgtgaacat catggctgcg tgatccatca gtctgaactg gaccagagca
      661 acgggcggtg gatctgtgaa aacacgggca tgtggacccg tgacggtctg acgtttttca
      721 gcgcccgggg tgatgaaatt ccgccgccgc gctccatcac gttccatatc tggacggcgt
      781 acagtccgtt caccacctgg gtacagattg tctatgactg gctggatgca ctgaaagatc
      841 ccaacggcct gaaaaccttt gtgaacacca cgctgggcga gacctgggaa gaggccgtgg
      901 gcgaaaaact cgatca
//
LOCUS       DAZFWL020000465          913 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFWL020000465 DAZFWL020000000
VERSION     DAZFWL020000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.471"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>913
                     /locus_tag="ACHKNP_RS27805"
                     /old_locus_tag="pgaptmp_005561"
     CDS             <1..>913
                     /locus_tag="ACHKNP_RS27805"
                     /old_locus_tag="pgaptmp_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4707478.1"
                     /translation="GVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLA
                     LAPWYGKKHRDNTLTMKRFTNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDDDIEQ
                     EGSPTFLGDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQ
                     YLKFGDKETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDG
                     ILWFSSSGEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGE
                     TWEAKIGERPDAEVMAERKEHYSAPVP"
BASE COUNT          218 a          228 c          263 g          204 t
ORIGIN      
        1 tgggtgttta tgcctacttc atagagcata agcagcgcaa cacccttatc tggttgccga
       61 cggatggcga tgccgagaac tttatgaaaa cccacgtcga gccgaccatc cgtgatattc
      121 catcgctgct ggcgctggcc ccgtggtatg gcaaaaagca ccgggataac acgctcacca
      181 tgaagcgttt caccaatggg cgtggtttct ggtgcctggg cggtaaagcg gcaaaaaact
      241 accgtgaaaa gtcagtggat gtggcgggtt atgatgaact tgctgccttt gatgatgata
      301 ttgaacagga aggctctccg acgttcctgg gtgacaagcg tattgaaggc tcggtctggc
      361 caaagtccat ccgtggctcc acgcccaaag tgagaggcac ctgtcagatt gagcgtgcag
      421 ccagtgaatc cccgcatttt atgcgttttc atgttgcctg cccgcactgc ggggaggagc
      481 agtaccttaa atttggcgat aaagagacgc cgtttggcct caaatggacg ccggatgatc
      541 cctccagcgt gttttatctc tgcgaacata atgcctgcgt catccgccag caggagctgg
      601 acttcactga tgcccgttat atctgcgaaa agaccgggat ctggacccgt gatggcattc
      661 tctggttttc gtcatccggt gaagagattg agccgccgga cagtgtgacc tttcacatct
      721 ggacggcgta cagcccgttc accacctggg tgcagattgt caaagactgg atgaaaacga
      781 aaggggatac gggaaaacgt aaaaccttcg tgaacaccac gctcggtgag acgtgggagg
      841 cgaaaattgg cgaacgtccg gatgctgaag tgatggcaga gcggaaagag cattattcag
      901 cgcccgttcc tga
//
LOCUS       DAZFWL020000466          907 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFWL020000466 DAZFWL020000000
VERSION     DAZFWL020000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.472"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..291)
                     /locus_tag="ACHKNP_RS27810"
                     /old_locus_tag="pgaptmp_005562"
     CDS             complement(<1..291)
                     /locus_tag="ACHKNP_RS27810"
                     /old_locus_tag="pgaptmp_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4707479.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDKASWHLVEDHRGKTVYDVASGDALFISELGPLPENV"
     gene            complement(291..>907)
                     /locus_tag="ACHKNP_RS27815"
                     /old_locus_tag="pgaptmp_005563"
     CDS             complement(291..>907)
                     /locus_tag="ACHKNP_RS27815"
                     /old_locus_tag="pgaptmp_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707480.1"
                     /translation="FDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFN
                     PSVFKDTYQYGYTSSGQNTWGVQGSVGMSTGWLANTSTDGNHSHSLSGTAASAGAHAH
                     TVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          226 a          220 c          213 g          248 t
ORIGIN      
        1 aacattttcc ggtaacggac cgagttcaga aataaataac gcgtcccctg acgctacgtc
       61 ataaaccgtt ttaccccgat ggtcttcaac gagatgccac gatgccttat cactgttgaa
      121 aacagccaca aagccagcag gaatatctgg tggtgcaata tcggtactgt ttgctggcag
      181 acctgtatga ggcggaatat atgcgtcacc ttcaccaata aattcattag ttccggccag
      241 cagattataa atttttatgg tccgtggttg ttcactcatt ctgaatgcca ttatgcaagc
      301 ctcacaatat agttaaatgc gatgtttttg acggtgtttt ccgcgttacc agcagcgtta
      361 acggtgatgg tgtgtccatg tgaaccaatc gcaacggagt gcgtatgagc accaataccg
      421 acagtatgcg cgtgtgcacc tgcagatgct gctgtgccgg acagtgaatg gctatgatta
      481 ccatctgtac tggtattcgc taaccacccc gtagacatac ctactgagcc ttgtacaccc
      541 caggtatttt gacctgagct tgtataacca tattgataag tatctttaaa aacactgggg
      601 ttaaatcgac ggccatctct atggctgtga ttaccagctg cattcgtgct gccacttaaa
      661 ctatgggtat gcgcaccagt gttattcgtg gatttagtgc cgtaatcaaa cgacgatgtg
      721 gttttcgtcc ccaaatccgt actggatgcg ctggcgctgt gggtatgcga tttaatgccg
      781 tcctgttcct gagacaatac ggcccgacca ctggcaggtt tgcccttaat cgtccagcca
      841 cgcatatcag ggatcacgcc tgacggataa gccgctgcaa gtttcgggta tgcagatttg
      901 tcaaaag
//
LOCUS       DAZFWL020000467          901 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFWL020000467 DAZFWL020000000
VERSION     DAZFWL020000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.473"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>901
                     /locus_tag="ACHKNP_RS27820"
                     /old_locus_tag="pgaptmp_005564"
     CDS             <1..>901
                     /locus_tag="ACHKNP_RS27820"
                     /old_locus_tag="pgaptmp_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HGU4707481.1"
                     /translation="NCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKFSRLTLGVLL
                     ALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNASLTTITTSVI
                     GANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDGGYLRLGSST
                     GGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQAGSNGQVVV
                     EKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGVGTLNVQDQD
                     SVITVRRLYNGYFGNGTLNISNNG"
BASE COUNT          242 a          167 c          251 g          241 t
ORIGIN      
        1 gaattgcact ctacaggtat ttcaggcctg ctcggaatta actcgcaggg caggtaaaac
       61 atcgacggtt aatttgcgta aatcctctgg actgacaacg aaattcagta gattgacgct
      121 gggtgttttg ctggcactaa gcggttcagc gtctggtgca agtctggaag ttgataatga
      181 tcagattacc aatattgata ctgatgttgc ttatgatgcc tacctggttg gctggtatgg
      241 cactggagtg cttaatattt tggctggcgg taatgcctcc ttaaccacta ttactaccag
      301 cgtcattggc gctaatgagg actcagaggg caccgttaat gttttgggtg gcacctggcg
      361 attgtatgat agcggaaata atgcaaggcc tttaaatgtg ggtcaatccg gaacggggac
      421 gctgaatatt aaacagaagg gtcacgtcga tggaggctat ttaagattag gttcttcgac
      481 aggaggcgtc gggacggtca atgttgaggg agaggactct gttctgacga ccgaattatt
      541 cgaaataggg agttacggta cgggctcatt aaatattacg gataagggtt acgtcacgag
      601 ttcaatcgtc gccattttag gctatcaggc gggcagtaat ggccaggttg tcgttgaaaa
      661 gggtggcgag tggctaataa aaaataacga ttcctcaatt gaatttcaaa ttggtaatca
      721 aggaactggg gaggcgacta ttcgcgaggg tggactgatt acggctgaaa atacgattat
      781 cggtggcaat gccaccggtg tcggaaccct gaatgtgcag gatcaagact ctgtcatcac
      841 ggtacgcaga ctctataatg gatatttcgg taatggcaca ctcaatattt ccaataatgg
      901 a
//
LOCUS       DAZFWL020000468          899 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFWL020000468 DAZFWL020000000
VERSION     DAZFWL020000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.474"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>899
                     /locus_tag="ACHKNP_RS27825"
                     /old_locus_tag="pgaptmp_005565"
     CDS             <1..>899
                     /locus_tag="ACHKNP_RS27825"
                     /old_locus_tag="pgaptmp_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707482.1"
                     /translation="SRLSIECHFSDDKREFIKMDLYNGGDVILESDTLQKINTFSKNI
                     TIQTADGVMANWDDESSTLSVYSIDMIAWRDKNKTEKEPLPLDVIQLTNWRIHNSCSL
                     FYDKYQVDIEANKLTYTILFPDTELPVQLNYASVIYGNHGAKYTFLDSGSKAITIHIT
                     DESGDYDTFDFRNIVFEHHTNDMFISFDNQGGFVISISNNATNELTDINVFRKNMTSF
                     DGCRSLIYLPSGDVFYISDIYKMSRGRKSVKLNVEKKPDINDIINVAILETSYLQIKK
                     IPNNDDGSYVLCLDNPNLSSYTL"
BASE COUNT          324 a          134 c          149 g          292 t
ORIGIN      
        1 tctcgcttat ctattgaatg ccactttagt gatgataaaa gagagtttat aaagatggac
       61 ttatataatg gaggtgatgt aattttagaa agtgacactt tacaaaaaat aaatacattt
      121 tcaaaaaata taacaataca gacagcggat ggagttatgg caaactggga tgatgaatca
      181 agtactctgt ctgtttattc aatagatatg attgcctgga gggataaaaa taaaactgaa
      241 aaagagcctt taccacttga tgtgattcag ttaactaatt ggaggataca taactcatgc
      301 tctttatttt atgataaata tcaagttgac attgaagcca ataaattaac gtatacaatt
      361 ttatttcctg atacggagtt acctgttcaa cttaattatg ctagtgttat ttatggtaat
      421 catggggcaa aatatacatt cctcgactca ggaagtaaag ctataaccat ccacattact
      481 gatgaaagtg gcgattatga tacttttgac ttccgcaata ttgtttttga acatcacacc
      541 aatgacatgt ttataagctt tgataatcaa ggaggatttg ttatatcaat atcaaataat
      601 gccacaaatg aactgacaga tattaatgta ttccgaaaaa acatgacttc ttttgatggc
      661 tgtcgttcat tgatatattt gcctagcggt gatgtttttt atatttcaga tatctataaa
      721 atgtccagag gaagaaaatc ggttaaatta aatgtagaaa agaaaccgga cattaacgac
      781 atcatcaatg tggctatttt agaaacttct tatctgcaaa taaaaaaaat cccaaataat
      841 gatgatggca gttatgtttt atgtctagac aaccctaacc tctcatctta cacattaca
//
LOCUS       DAZFWL020000469          896 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFWL020000469 DAZFWL020000000
VERSION     DAZFWL020000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.475"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(25..189)
                     /gene="kil"
                     /locus_tag="ACHKNP_RS27830"
                     /old_locus_tag="pgaptmp_005566"
     CDS             complement(25..189)
                     /gene="kil"
                     /locus_tag="ACHKNP_RS27830"
                     /old_locus_tag="pgaptmp_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGU4707483.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILIQKLRSSK
                     SIHYPPFLFS"
     gene            complement(158..322)
                     /locus_tag="ACHKNP_RS27835"
                     /old_locus_tag="pgaptmp_005567"
     CDS             complement(158..322)
                     /locus_tag="ACHKNP_RS27835"
                     /old_locus_tag="pgaptmp_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HGU4707484.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            complement(395..763)
                     /locus_tag="ACHKNP_RS27840"
                     /old_locus_tag="pgaptmp_005568"
     CDS             complement(395..763)
                     /locus_tag="ACHKNP_RS27840"
                     /old_locus_tag="pgaptmp_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HGU4707485.1"
                     /translation="MSNIKKYIIDYDWKTSIEIEIDHDLMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDPDDMTIKAA"
BASE COUNT          245 a          188 c          185 g          278 t
ORIGIN      
        1 atcgcccgcg aaatgccagg ctgattagga aaacaggaaa ggggggtagt gaatgctttt
       61 gcttgatctc agtttctgta ttaatatcca ttttttataa gcgtcgacgg cctcacgaaa
      121 catcttttca tcgccaataa aagtggcgat agtgaattta gtctggatag ccataattgt
      181 ttgatccatt tttcgggact cctggctgat taagtatgtc gataaggcgt ttccatccgt
      241 cacgtaattt acgggtaatt cgttcaagta aagattcgga agggcagcca gcaacaggcc
      301 accctgcaat ggcatattgc atggtgtgct ccttatttat acataacgaa aaacgcctcg
      361 agtgaagcgt aattggtatg cggtaacgcc gcgctcaggc ggctttgata gtcatatcat
      421 ctggatcaaa tattcctgat gtatcgatat cggtaattct tattccttcg ctaccatcca
      481 ttggaggcca tccttcctga ccatttccat cattccagtc gaactcacac acaacaccat
      541 atgcatttaa gtcgcttgaa attgctataa gcagagcatg ttgcgccagc atgattaata
      601 cagcatttaa tacagagccg tgtttattga gtcggtattc agagtctgac cagaaattat
      661 taatctggtg aagtttttcc tctgtcatta agtcatggtc gatttcaatt tctattgatg
      721 ttttccagtc gtaatcaatg atgtattttt tgatgtttga catctgttca tatcctcaca
      781 gaaaaaaatc gccctcacat tagagggcaa agaagatttc caataatcag aacaagtcgg
      841 ctcctgttta gttacgagcg acattgctcc gtgtattcac tcgttggaat gaatac
//
LOCUS       DAZFWL020000470          895 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFWL020000470 DAZFWL020000000
VERSION     DAZFWL020000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.476"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..153)
                     /locus_tag="ACHKNP_RS27845"
                     /old_locus_tag="pgaptmp_005569"
     CDS             complement(<1..153)
                     /locus_tag="ACHKNP_RS27845"
                     /old_locus_tag="pgaptmp_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4707486.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGASSLSVPQ
                     QQVQFDA"
     gene            complement(143..>895)
                     /locus_tag="ACHKNP_RS27850"
                     /old_locus_tag="pgaptmp_005570"
     CDS             complement(143..>895)
                     /locus_tag="ACHKNP_RS27850"
                     /old_locus_tag="pgaptmp_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4707487.1"
                     /translation="SELDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNNVTLGG
                     VKIPHLFPGDDLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSA
                     RASANESWRYFMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSR
                     AEWIGAGRMAIDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQK
                     AGLSRPVWIAQAYEQQIAESRRPEEETTPRET"
BASE COUNT          207 a          270 c          217 g          201 t
ORIGIN      
        1 agcatcaaac tgtacctgct gttgtggtac cgaaagactt gatgccccca tctcgcgccc
       61 gagcgcgcaa aagaaaaccc gcgcataggc gggctccaga agcagcggtt cattgaatgc
      121 tgcggcaata atgtgtgaaa gattacgtct cacgtggtgt tgtctcctct tccggcctgc
      181 gactctccgc tatctgctgc tcatacgcct gcgctatcca caccggacgt gagagtccgg
      241 ctttttgtcg ctcagcagat tccctgacct gctggcggaa aatgtcctga taatcctcgc
      301 ccatcagcgc cagctctttc tcatacgtgc tcagtccggc ctcaatgcgc atcactgatt
      361 cctggacttc cttgagcccg tcaatggcca ttcttccggc tccaatccac tcagcccgtg
      421 accaggctga tcgcgcctga taaaaatcaa aacgcgcccg tggcggacga ataatccccc
      481 gaagaagtgc ctcttccagc cagcaggaaa acatctgcgt ggccagccgg gacgcaataa
      541 attttcgtcg tcccataaaa tagcgccacg actcattggc ggaggcgcgg gcacttgaat
      601 aactgacctt cgagtaatcc cgggacaact gttcgtagga aacgccaaga ccggcggcga
      661 tataccgcag cagcgcctgt tcaagcgccg aaaatccatt gtctgaatcc tgcgcagtct
      721 gcagtttcag atcatcaccg gggaaaaggt gcggaatttt gacaccgccc agcgtcacgt
      781 tattcgtgtc ataccaggtg gagaacttat ccagaatatt aataagcgga ttatccttct
      841 gcccctgcgg cgcaccggcg atatattcaa aggccttttc ggtatcaagt tcact
//
LOCUS       DAZFWL020000471          893 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFWL020000471 DAZFWL020000000
VERSION     DAZFWL020000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.477"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(6..140)
                     /locus_tag="ACHKNP_RS27855"
                     /old_locus_tag="pgaptmp_005571"
     CDS             complement(6..140)
                     /locus_tag="ACHKNP_RS27855"
                     /old_locus_tag="pgaptmp_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707488.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLIRAAKKQLEALCAMK
                     "
     gene            complement(216..527)
                     /locus_tag="ACHKNP_RS27860"
                     /old_locus_tag="pgaptmp_005572"
     CDS             complement(216..527)
                     /locus_tag="ACHKNP_RS27860"
                     /old_locus_tag="pgaptmp_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein"
                     /protein_id="HGU4707489.1"
                     /translation="MKLRVWHIPQVPMKPFIAEVASVEEGVRLMGALADYDAFQYDNN
                     IKPDYCNANGLEMWDESLTDEDLSEVGLTDRWVDWYSECQCYDDPRKYLESLKEETSA
                     A"
     gene            complement(671..>893)
                     /locus_tag="ACHKNP_RS27865"
                     /old_locus_tag="pgaptmp_005573"
     CDS             complement(671..>893)
                     /locus_tag="ACHKNP_RS27865"
                     /old_locus_tag="pgaptmp_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707490.1"
                     /translation="KVAKNAERSFAFPTKKLAIESLLRRKKYHLMRIKQDLAVVSTLV
                     DGMKNIDTSTPDIEYNFGHNQETENWVFY"
BASE COUNT          231 a          204 c          178 g          280 t
ORIGIN      
        1 aatggctatt tcattgcgca taacgcctcc agttgtttct ttgctgctct aattaattgt
       61 ttaactcggc gtgataattc agattcgtgc gggtagaaag cggacatgac gccgctaccc
      121 gcgagctgaa agtgcatcat gggtaactcc ttatatttga ttgcataacg aaaacgcctc
      181 gagtgaagcg ttattggtat gcggtaaagc cgcgcttagg cggctgatgt ttcttctttc
      241 aggctttcga gatatttacg tgggtcgtcg taacattggc attcgctgta ccaatccacc
      301 cagcgatcag taagccccac ctctgataaa tcttcatcgg taaggctctc atcccacatc
      361 tcaaggccgt tagcattgca gtaatcaggc ttgatgttgt tgtcatactg aaaggcgtca
      421 taatcagcca gtgcgcccat caggcgaaca ccctcttcaa cacttgccac ttctgcaatg
      481 aacggtttca taggtacttg cgggatatgc cagacacgta atttcatatt tcctccgtca
      541 aaaaaatcgc cctcacattg gagggcaaag aagatttcca ataatcagaa caagtcggct
      601 cctgtttagt tacgagcgac attgctccgt gtattcactc gttggaatga atacacagtg
      661 cttattcgta ctaataaaat acccaatttt ctgtttcttg gttgtgtcca aagttatatt
      721 caatatctgg tgttgatgta tcaatatttt tcatcccatc aacaagagtt gatacaacag
      781 ccaaatcttg ttttattctc attaaatggt atttcttccg gcgcaataaa ctttcaatag
      841 caagtttctt cgttgggaat gcaaaagatc tttctgcatt ttttgctact ttc
//
LOCUS       DAZFWL020000472          892 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFWL020000472 DAZFWL020000000
VERSION     DAZFWL020000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.478"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            176..313
                     /locus_tag="ACHKNP_RS27870"
                     /old_locus_tag="pgaptmp_005574"
     CDS             176..313
                     /locus_tag="ACHKNP_RS27870"
                     /old_locus_tag="pgaptmp_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C551"
                     /protein_id="HGU4707491.1"
                     /translation="MSGLEGFGTGFRSYVQAQGTLVNNTNLTDETGKVLNPATPEEIK
                     Y"
     gene            417..>892
                     /locus_tag="ACHKNP_RS27875"
                     /old_locus_tag="pgaptmp_005575"
     CDS             417..>892
                     /locus_tag="ACHKNP_RS27875"
                     /old_locus_tag="pgaptmp_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HGU4707492.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
                     GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIS
                     EQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIV"
BASE COUNT          257 a          184 c          198 g          253 t
ORIGIN      
        1 ttaaatactt agcgtatggg gaagttgatt ttagatggtc cgaagtttta agaggtctaa
       61 ggatggggtg tgtgggaggt ggaattctag gttttggtat agtcttgttt cgctttcttg
      121 gcataaaagg attttaagtg aagataaggt acgatttgtt gttgagaata attcaatgtc
      181 tggcctggaa gggtttggga ccggcttccg gagttatgtt caggcccagg ggacgctggt
      241 aaataatacc aatctcactg atgaaactgg caaagtgctg aatccggcaa cgccagagga
      301 aattaagtac tgatgaatcc cctaatgatt ttggtaaaaa tcattaagtt aaggtggata
      361 cacatcttgt catatgatca aatggtttcg cgaaaaatca ataatcagac aacaagatgt
      421 gcgaactcga tattttacac gactctcttt accaattctg ccccgaatta cacttaaaac
      481 gactcaacag cttaacgttg gcttgccacg cattacttga ctgtaaaact ctcactctta
      541 ccgaacttgg ccgtaacctg ccaaccaaag cgagaacaaa acataacatc aaacgaatcg
      601 accgattgtt aggtaatcgt cacctccaca aagagcgact cgctgtatac cgttggcatg
      661 ctagctttat ctgttcgggc aatacgatgc ccattgtact tgttgactgg tctgatatta
      721 gtgagcaaaa acgacttatg gtattgcgag cttcagtcgc actacacggt cgttctgtta
      781 ctctttatga gaaagcgttc ccgctttcag agcaatgttc aaagaaagct catgaccaat
      841 ttctagccga ccttgcgagc attctaccga gtaacaccac accgctcatt gt
//
LOCUS       DAZFWL020000473          870 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFWL020000473 DAZFWL020000000
VERSION     DAZFWL020000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.479"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            101..>870
                     /locus_tag="ACHKNP_RS27880"
                     /old_locus_tag="pgaptmp_005576"
     CDS             101..>870
                     /locus_tag="ACHKNP_RS27880"
                     /old_locus_tag="pgaptmp_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HGU4707493.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALLEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAASCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLISKYAEHTPLYRQSEMYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPG"
BASE COUNT          185 a          260 c          255 g          170 t
ORIGIN      
        1 tatcgactgg cggcagccta aaagactgct tacgtccctg actatgttgt aagcctcttt
       61 atcctggtcg acgctgaatg agcctggtaa tatacccggt atgagcagct cacttcctga
      121 cgatatcaat gcactgaaac gtctccttgc cgaacaggag gcgctgaacc gtgccctgct
      181 ggaaaagctg aacgagcgtg aacgcgaaat agaccatctg caggcacagc tggataagct
      241 gcgccggatg aacttcggca gccgctccga aaaagtctcc cgtcgtatcg cacagatgga
      301 agctgacctg aaggcacttc agaaagaaag tgataccctt accggtcggg ttgacgaccc
      361 ggccgtgcag cgcccgctgc gtcaaacccg cacccgcaaa ccgttccccg aatcactccc
      421 ccgcgatgaa aaacggctgc tgccggcagc atcatgctgc ccggaatgtg gaggctcact
      481 gagctatctg ggtgaggatg ccgccgaaca gctggagttg atgcgcagcg ccttccgggt
      541 tatccggact gtacgtgaaa agcatgcctg tactcagtgc gatgccatcg tgcaggcccc
      601 cgcgccttca cggcccatcg agcggggtat cgcaggaccg gggctgctgg cccgcgtgct
      661 gatctcaaag tatgcagagc acaccccgct gtaccgccag tctgaaatgt acggccgcca
      721 gggcgtggag ctgagtcgtt cactgctgtc gggctgggtg gatgcatgct gccggctact
      781 gtcaccgctg gaagaagcgc ttcaggacta tgtgctgact gacggtaagc tccatgctga
      841 tgacacgcct gtcccggtgc tgttgccagg
//
LOCUS       DAZFWL020000474          863 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFWL020000474 DAZFWL020000000
VERSION     DAZFWL020000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.480"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(409..>863)
                     /locus_tag="ACHKNP_RS27885"
                     /old_locus_tag="pgaptmp_005577"
     CDS             complement(409..>863)
                     /locus_tag="ACHKNP_RS27885"
                     /old_locus_tag="pgaptmp_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HGU4707494.1"
                     /translation="KSDYKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKD
                     MPLRKQAEILFCLGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSV
                     YYYNDILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          317 a          192 c          105 g          249 t
ORIGIN      
        1 aattaaaatc atatttcatc tatagtgacc attcacagca aaacaaaatc aattatcaca
       61 ttcaaaaaaa atactgacaa caaagatact cttcagacaa agttgtcata tgtaaatttt
      121 cccttaaaac gttgctcaaa aaaaatataa aaatcatatg ccctaatcaa catcctcaag
      181 cactacgata tatgcagtat cctattcata tattaccacc atatcagaca aatcgaatca
      241 caacgagttc tatcgtactc tttcatcttc gacactgtaa attttaagaa cattcggctc
      301 atgccactaa accaagacat aataacaacg gagcaataaa tgttcccact gaacatgtgc
      361 aaacctaaaa agacagtgtt acaataatat tcatccatgg tctctaaatc aacgccacgc
      421 aacaggataa tacaatgaaa atatttcagg aaaagattct tttgcatgct ccgttagcat
      481 atcagtcagc acagcggtac aagtaaaaac attatttctt ccttgtagcc tgtctaaaat
      541 atcattgtaa taataaactg agccaaatac ctgaggatct gttttataag cttgttcaat
      601 caatccattt gcatatctcc gtagaatctc aggagaatca tactctgtac caaacatatc
      661 actcgatgag tatttacaga aaaccccccc taaacaaaat aatatttcag cctgttttct
      721 tagtggcata tctttcaata catggctccc cataatcata tctacatgct gagcgttcag
      781 ttcccaacca tcaagaaagg gattccagac acaggcaagt ttcctttgct gatcatcagc
      841 aatcatttta taatctgatt tat
//
LOCUS       DAZFWL020000475          862 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFWL020000475 DAZFWL020000000
VERSION     DAZFWL020000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.481"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..670
                     /locus_tag="ACHKNP_RS27890"
                     /old_locus_tag="pgaptmp_005578"
     CDS             <1..670
                     /locus_tag="ACHKNP_RS27890"
                     /old_locus_tag="pgaptmp_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4707495.1"
                     /translation="QRADWQKGLQEGLSNWVDNASDYASQAAQLATEGISGMVNNITE
                     MLNGNKVEWRNWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFIGSIGDWLGGAV
                     ANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVR
                     AVGSMNGSAGLVYSPVYHIAIQNDGANGQIGAEAAGSLVQLIDQRVQAVMLSMRRDGG
                     MLSG"
     gene            663..>862
                     /locus_tag="ACHKNP_RS27895"
                     /old_locus_tag="pgaptmp_005579"
     CDS             663..>862
                     /locus_tag="ACHKNP_RS27895"
                     /old_locus_tag="pgaptmp_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707496.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDEFLS"
BASE COUNT          195 a          195 c          304 g          168 t
ORIGIN      
        1 gcagcgtgcg gactggcaga aaggcttgca ggaggggctc agtaactggg tggacaatgc
       61 atccgattac gcttcacagg cagcacagct tgcgacggag ggtatctcag ggatggtgaa
      121 taacatcacg gagatgctga acggaaacaa agtggaatgg cgcaactggg cctcatccgt
      181 actgcaggaa atctcaaaag ttcttatgaa tgccgcgatt gtcaacggaa ttaagacggc
      241 ggcaaacggt atgtccggtg cgggaggatt tattggcagc attggtgact ggctgggcgg
      301 tgcggtggcc aatgcaaaag gcggcgtgta tacctcggca aacctgagtg cgtacagcaa
      361 cagcattgtg gacacgccca cgtactttgc gtttgcaaaa ggggccgggc tgatggggga
      421 agccggacct gaagccatta tgcccctgac ccgggcggcg gatggctcgc tgggcgtacg
      481 cgcggtgggc agtatgaacg gcagtgccgg tctggtgtat tccccggtct accacatcgc
      541 cattcagaat gacggggcta acggacagat aggggcggag gcggcaggca gtcttgtgca
      601 gctgattgac cagcgggtgc aggcggtgat gctgtccatg cgacgtgacg gaggaatgct
      661 gagtggctga gataaaaacg ctgcatctgg tcccgcgtga agggatgcag gtgagtgaga
      721 aaccgtcggt ggtgagggtt cggtttggtg acggttatga acagcgccga ccgacgggac
      781 ttaatgccag actgaagacg tttcaggcgg tgttccgggt gacggatgaa ccaacccggc
      841 gctggctgga tgaattttta tc
//
LOCUS       DAZFWL020000476          851 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZFWL020000476 DAZFWL020000000
VERSION     DAZFWL020000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.482"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            18..65
                     /locus_tag="ACHKNP_RS27900"
                     /old_locus_tag="pgaptmp_005580"
     CDS             18..65
                     /locus_tag="ACHKNP_RS27900"
                     /old_locus_tag="pgaptmp_005580"
                     /inference="COORDINATES: protein motif:HMM:NF017611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HGU4707497.1"
                     /translation="MSDPNSSMYEEEYDG"
     gene            58..663
                     /locus_tag="ACHKNP_RS27905"
                     /old_locus_tag="pgaptmp_005581"
     CDS             58..663
                     /locus_tag="ACHKNP_RS27905"
                     /old_locus_tag="pgaptmp_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU4707498.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHSRHRWTIEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            660..>851
                     /locus_tag="ACHKNP_RS27910"
                     /old_locus_tag="pgaptmp_005582"
     CDS             660..>851
                     /locus_tag="ACHKNP_RS27910"
                     /old_locus_tag="pgaptmp_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HGU4707499.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDAL"
BASE COUNT          294 a          196 c          216 g          145 t
ORIGIN      
        1 actgctgtgc agaactgatg agcgatccga atagctcaat gtacgaggaa gaatacgatg
       61 gctaaaccag cgagaaggaa atgcaaaata tgcaaggaat ggtttcaccc ggcattctca
      121 aatcagtggt ggtgctgccc ggaacacgga actcaattag cactcgaacg acgaagtaaa
      181 gaacgcgaaa aagcggaaaa agcagcagag aagaaacgac gacgagagga gcagaaacag
      241 aaagataaac ttaagattcg aaaactcgcc ttaaagcccc gcagttactg gattaaacaa
      301 gcccaacaag ctgtaaacgc cttcatcaga gaaagagacc gcgacttacc atgtatctcg
      361 tgcggaacgc tcacgtctgc tcagtgggat gccggacatt accggacaac tgctgcggca
      421 cctcaactcc gatttgatga acgcaatatt cacaagcaat gcgtggtgtg caaccagcac
      481 aaaagcggaa atctcgttcc gtatcgcgtc gaactgatta accgcatagg gcaggaagca
      541 gtagacgaaa tcgaatcaaa ccatagccgc catcgctgga ctatcgaaga gtgcaaggcg
      601 atcaaggcag agtaccaaca gaaactcaaa gacctgcgaa atagcagaag tgaggccgca
      661 tgacgttctc agtaaaaacc attccagaca tgctcgttga agcatacgga aaccagacag
      721 aagtagcacg cagactgaaa tgtagtcgcg gcacggtaag aaaatacgtt gatgataaag
      781 acgggaaaat gcacgccatc gtcaacgacg ttcttatggt tcatcgcgga tggagtgaaa
      841 gagatgcgct a
//
LOCUS       DAZFWL020000477          848 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFWL020000477 DAZFWL020000000
VERSION     DAZFWL020000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.483"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>848
                     /locus_tag="ACHKNP_RS27915"
                     /old_locus_tag="pgaptmp_005583"
     CDS             <1..>848
                     /locus_tag="ACHKNP_RS27915"
                     /old_locus_tag="pgaptmp_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4707500.1"
                     /translation="PKNVLFAVLWMQGEFDFGGTPVNHAAQFGALVDKFRADLADMAG
                     QCVGGSAGGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENGVNV
                     PTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAILRHA
                     GRVALNAGASSTVSEVRPSSPSGAEATGVTALLSYLASESEGSLKVQGWSASGGRAEV
                     VSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKVSGALAANQYV
                     MAFYWPV"
BASE COUNT          198 a          195 c          284 g          171 t
ORIGIN      
        1 ccgaaaaatg tgctgtttgc cgtgttgtgg atgcaggggg aatttgattt tggcggtacg
       61 ccggtaaatc acgcagcaca gtttggtgcg ctggttgata aattccgtgc agacctggcg
      121 gatatggcag gtcagtgcgt cggtggctct gctggcggtg ttccctggat atgtggagat
      181 acgacgtatt tctggaagca gaagaacgaa tcctcgtacc agacggtgta cggcagctac
      241 aaaaacaaaa cggaaaagaa tatccatttc gtaccgttca tgacggatga gaacggggtg
      301 aatgtgccga cgaacaaacc ggaagaagac ccggacattc cgggtatcgg atattacggt
      361 tcgaaatggc gtgacagctc agccacctgg acgtcacagg acagggcgag ccatttcagt
      421 tcatgggctc gccgcgggat tatttccgac cgtctggcaa cggcgatttt gcgccatgcg
      481 ggaagagtgg cgctaaacgc gggggcatca tcgacagtat cagaggtgcg cccgtcatcg
      541 ccttccggtg cagaagccac aggcgtcaca gcactgctct cttaccttgc cagcgagtca
      601 gagggaagcc tgaaagtaca gggatggtca gccagtggcg gcagggcaga agtggtcagc
      661 gatgcggagg gaaccggagg taaggcagtg aagctgacca aggaagccgg taaaagcagc
      721 tgggtgctgg agtacgccgc gggcaacggt gcggctctgt tacagaaagg ggggcagatt
      781 cgctgccgct ttaaggtttc gggagcgctg gctgcgaacc agtatgttat ggcgttttac
      841 tggccggt
//
LOCUS       DAZFWL020000478          843 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFWL020000478 DAZFWL020000000
VERSION     DAZFWL020000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.484"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..425)
                     /locus_tag="ACHKNP_RS27920"
                     /old_locus_tag="pgaptmp_005584"
     CDS             complement(<1..425)
                     /locus_tag="ACHKNP_RS27920"
                     /old_locus_tag="pgaptmp_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU4707501.1"
                     /translation="MSQPVGDLIIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTATAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMLRGLAGAITLPMVGVTSLAVATGALAYAWYQG"
     gene            complement(406..>843)
                     /locus_tag="ACHKNP_RS27925"
                     /old_locus_tag="pgaptmp_005585"
     CDS             complement(406..>843)
                     /locus_tag="ACHKNP_RS27925"
                     /old_locus_tag="pgaptmp_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4707502.1"
                     /translation="FALRLAREMGRPDWRAMLAGMTSTEYADWRHFYRTHYFQDTQLD
                     MHFSGLMYAVLSLFFCDPDMHPSDFSLLVPRHEEEQVERLDEDKMLMQKAAGLAGGVR
                     FGGDGGGDILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
BASE COUNT          192 a          279 c          212 g          160 t
ORIGIN      
        1 ccctggtacc aggcgtacgc cagcgcacct gtcgccaccg ccagcgaggt gaccccgacc
       61 atcggcaggg tgatcgcacc ggcaagcccc ctgagcatgg ggatcatccc gccgaaggag
      121 tccttcacct gaccgccctg ttgcagcagg atcagccagg gattctgacc accggcaagc
      181 tgcgtggcga tatccgtaaa ctgtgcgggc agggtgcgca tggccgcttt atactgtccg
      241 acggaaatcc cggctttttg cgcagccagc gcctgacggc tcaggccctg ttcaacagca
      301 gtggcggttt ttctggcgtc agtatccagt cctgaaaaat gacgccttac ccggctcatc
      361 tgctcatcga aacggaccgc atccagactc aggtcaataa taagatcacc aaccggctgg
      421 gacatatctc acacctcccg gaatccccgc tgaagccatc attaatgcgg catcatccac
      481 catgacatcc gccacatccg cagacgataa aatatcgccc cctccgtccc caccgaaccg
      541 gacgcctccg gcaagtcctg ccgctttctg catcagcatt ttgtcctcat ccagcctctc
      601 cacctgctct tcctcatgcc gggggacaag cagactgaaa tcagagggat gcatatccgg
      661 atcgcaaaaa aacaggctga gtacagcgta catcagcccg gaaaaatgca tatccagttg
      721 ggtatcctga aaataatgcg tgcggtaaaa atgtcgccag tcggcatatt cggtggatgt
      781 catcccggca agcatggcgc gccagtcagg cctccccatc tctcgcgcca gtctgagggc
      841 aaa
//
LOCUS       DAZFWL020000479          843 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFWL020000479 DAZFWL020000000
VERSION     DAZFWL020000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.485"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(61..201)
                     /locus_tag="ACHKNP_RS27930"
                     /old_locus_tag="pgaptmp_005586"
                     /pseudo
     CDS             complement(61..201)
                     /locus_tag="ACHKNP_RS27930"
                     /old_locus_tag="pgaptmp_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            590..>843
                     /locus_tag="ACHKNP_RS27935"
                     /old_locus_tag="pgaptmp_005587"
     CDS             590..>843
                     /locus_tag="ACHKNP_RS27935"
                     /old_locus_tag="pgaptmp_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein"
                     /protein_id="HGU4707503.1"
                     /translation="MLPTSQLRPSGTFYSNSAETSAGIKSEITPTQIEEARAIGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSV"
BASE COUNT          265 a          178 c          143 g          257 t
ORIGIN      
        1 tggtagcccc tttgcttttc tggctcttgg tataaacttg gtataccaat tggtatattt
       61 ttattcggat gatcttggtt cttccggcca ctcaatatca ggtgcagttg atgtatcaac
      121 acggttcagc aacacccgat acttcttcca ggcttccagc aacgagtttt cttcctccgt
      181 tgcgatctcc agatctactg aagaaggcaa cagaaataga taattagtgc tttatattca
      241 aaaggttaat agcaaatttg gcatgctttt tactgtctag taaacagcat gcctctatcc
      301 ataaaatcaa acagttgcaa tctaattttg gagaacgttt tttcttctcc cttttagcaa
      361 atcgctataa aaaacaacaa atggtataaa aatgagcaat cctaatccat gcatgacgga
      421 ctggagaaag tattcacaaa agttaaaatc aacagtctgt tgattttgag atataactct
      481 cttttctaat attgacacct tggctttgct ggttgctaaa attagcttgt tgattagcag
      541 tatggataga acttacactg ttatttactt ctaaaaatgg aatttatcaa tgcttcctac
      601 atcgcaatta cgaccgtccg ggacattcta ctccaattcc gcagaaacat cagcaggcat
      661 caaaagcgaa atcacaccaa ctcagataga agaagcgcgg gccattggtc gtttatatat
      721 caaagattgt gatattgagt atctgccaca gttaccaaac gaaataacat cagttacaat
      781 cgaaaattgc aataacctga ccacccttac aggattaccg gttaatacac aaaacctgtc
      841 cgt
//
LOCUS       DAZFWL020000480          842 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFWL020000480 DAZFWL020000000
VERSION     DAZFWL020000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.486"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..495
                     /locus_tag="ACHKNP_RS27940"
                     /old_locus_tag="pgaptmp_005588"
     CDS             61..495
                     /locus_tag="ACHKNP_RS27940"
                     /old_locus_tag="pgaptmp_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HGU4707504.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT          198 a          185 c          258 g          201 t
ORIGIN      
        1 gtactgcaca cactgccaga ccggaggatt cagtattccc cggacaactc aggaggaaat
       61 atgcgaattc tgaactgtta tatggcgaat gacagcaaag gccattttgt tacggcgaaa
      121 gaagctgcga agcacaaccg acaggacgtt ttgtgctgtg tgtcctgtgg atgcccgtta
      181 acacttcagc ggggcaatga cggacaacca ccgtggtttg aacatgacca gatgactgtc
      241 gctgaaaaaa tcctgctgcg atgtacctgg cttgacccgg cggagaaaga ggcccgtcgt
      301 ttgcatctgc agggcatgac ggttccggat tatacggtga aggtgagaaa gtggttttgt
      361 gtgatgtgtg acgaagatta tgagggggaa aagtgctgcc cacgctgcgg taccggggta
      421 tacagcaggg cgtgggggcg gcaggaggtg ccgtcggaag atgccagggc tgataatccg
      481 ttacagaggc tgtaatggtt gcctccggag cagtttgcgg tgatgccttt cccgggaatg
      541 gattgccagt gcggggactg tggagcttaa ttccggtgtt ggtggccttt ccgttgattt
      601 tatgccaaca gccccctgtg actgacaggc tgcctcgtca ttccattcgt atctccagta
      661 acagggggtt gtattgtatt ttattgtgcc ggttcaggtg tgcgtttccc ggcgcgtctg
      721 caccggctta accaaattca acagggataa aatagtggta agcgtacagc ctgaaccgtc
      781 tggtcagaat ctgacgaatt agacaaagtg gtgtccacca aataagtagt gggaaccaaa
      841 gt
//
LOCUS       DAZFWL020000481          841 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFWL020000481 DAZFWL020000000
VERSION     DAZFWL020000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.487"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            39..308
                     /locus_tag="ACHKNP_RS27945"
                     /old_locus_tag="pgaptmp_005589"
     CDS             39..308
                     /locus_tag="ACHKNP_RS27945"
                     /old_locus_tag="pgaptmp_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU4707505.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(606..>841)
                     /locus_tag="ACHKNP_RS27950"
                     /old_locus_tag="pgaptmp_005590"
     CDS             complement(606..>841)
                     /locus_tag="ACHKNP_RS27950"
                     /old_locus_tag="pgaptmp_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU4707506.1"
                     /translation="YQSDIFLPPGCIFTPTRTGVILNNKELPVTIYNVTPFNKNVWNL
                     IKSSQECPANTGNITDKCFNHRCILEICPYGRK"
BASE COUNT          225 a          153 c          219 g          244 t
ORIGIN      
        1 cagtttttat atacaacggg taaataaagg agttaagtat gaagaagatg tttatggcgg
       61 ttttatttgc attagtttct gttaatgcaa tggcggcgga ttgtgctaaa ggtaaaattg
      121 agttttccaa gtataatgag gatgacacat ttacagtgaa ggttgacggg aaagaatact
      181 ggaccagtcg ctggaatctg caaccgttac tgcaaagtgc tcagttgaca ggaatgactg
      241 tcacaatcaa atccagtacc tgtgaatcag gctccggatt tgctgaagtg cagtttaata
      301 atgactgagg cataacctga ttcgtggtat gtgggtaaca agtgtaatct gtgtcacaat
      361 tcagtcagtt gacagttgcc tgtcagactg agcatttgtt aaaaaaattt cgcatggtga
      421 atccccctgt gcggaggggc gactggtgta taaggtgtca tctctgacga caacctggat
      481 cgagatacgc gggttcaggg acaccggact gaactcaccg ggaggcaccc ggcaccatgt
      541 tcatggtgat acagaaatgc ggcttcagcc cctctccgga ggggcttttt atctgaatga
      601 ttctgttatt ttcgcccgta tgggcatatt tccagaatgc agcgatgatt aaaacactta
      661 tctgtgatat ttcctgtgtt tgcagggcat tcctggctgc ttttaattaa attccagacg
      721 tttttattaa atggggtcac gttgtaaatg gtgacgggga gctctttatt attgagtatt
      781 acgcctgtcc gggttggtgt aaaaatacag cccggaggga ggaagatatc agactgatac
      841 c
//
LOCUS       DAZFWL020000482          840 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFWL020000482 DAZFWL020000000
VERSION     DAZFWL020000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.488"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            39..>840
                     /locus_tag="ACHKNP_RS27955"
                     /old_locus_tag="pgaptmp_005591"
     CDS             39..>840
                     /locus_tag="ACHKNP_RS27955"
                     /old_locus_tag="pgaptmp_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4707507.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVN"
BASE COUNT          273 a          167 c          165 g          235 t
ORIGIN      
        1 gtgatctcca tttcgattga tttagtgttt attgacgtat gtactggatt attaacgata
       61 atatcgagtt ctggcctgag caccgaaaat taatatcggt acataacgcc gatcttaacg
      121 tcgttctgac aacgccagcc agtcgatgtt tatcacttct acttgaagct tttcctgatg
      181 tggttgcaca acaagatttt ttcaccagag tctgggaaga agaaggtatg cgtgtgccta
      241 ctaacacgtt atatcagaac atatccatta tcagacgcgg atttcgcgct gttggtgata
      301 ctacccactc gctaattgca accgtgccga gaagaggatt caagatccat aatgacatta
      361 acatacaaaa tcatgtaata aactcgtcaa cagacgcaca tacacacaat gccccacctg
      421 ccataaaagt taatgcggga tacaaagaga gcattggtgg cgcaaagaat ttcaataaca
      481 aaatcctcaa acatataaaa agtcatctaa ttatgttgag cgcatttgtc ataggcgcat
      541 attctgcata ttggctatgg aataataatc aaccgaagcc atttttcaag gattacaaga
      601 ctgttgcgga aattaatggc tgccatttta atgtaacaga agatacaatt gacgggttga
      661 aagagttcga taaatataaa acacggatac tggattcggg gattaactgt aaaaaacatc
      721 cgtggttata cttccctctt gcaaaatcct caccggggat gattgttatg gcatgcaata
      781 aaaattataa ccaacatgaa gtggcagact gtttaaccct gtcttaccgt gaggttaatc
//
LOCUS       DAZFWL020000483          834 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFWL020000483 DAZFWL020000000
VERSION     DAZFWL020000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.489"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..746
                     /locus_tag="ACHKNP_RS27960"
                     /old_locus_tag="pgaptmp_005592"
     CDS             <1..746
                     /locus_tag="ACHKNP_RS27960"
                     /old_locus_tag="pgaptmp_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HGU4707508.1"
                     /translation="VSVINPALGKAFAQSEGLRNKTDTVDARMLAEFCRQKRPAAWEA
                     PHPLERALRALVVRHQALTDMHTQELNRTETAREVQRPSIDAHLLWLEAELKRLEKQI
                     KDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGLKDRFAHARQFAAFAGLTPRRY
                     ESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEWGRAFRDRLAANGKKGKVILGA
                     MMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          204 a          205 c          267 g          158 t
ORIGIN      
        1 tagtgtcagt cattaatcct gcgctgggta aagctttcgc tcagagtgaa ggactgcgta
       61 acaagactga taccgtggat gcgcgcatgc tggcagagtt ctgtcgtcag aagcgccctg
      121 cagcctggga agcgcctcac ccgcttgaac gcgcgttgcg tgccctggta gtccgccacc
      181 aggcgctgac agatatgcac acgcaggaac tgaatcgcac tgaaacggcg cgggaagtcc
      241 agagaccgag cattgatgct caccttctgt ggcttgaagc agagctgaag cgtcttgaga
      301 agcagataaa agacctgaca gacgatgatc cggatatgaa acaccgcagg aaactgctgg
      361 aaagcatccc gggtatcgga gagaaaacat ctgcggtatt gctggcttat atcggtctga
      421 aggaccgctt cgcccatgcc agacagttcg ccgcttttgc gggtctgaca ccacggcgtt
      481 atgaatcagg tagcagtgtg agaggggcga gccggatgag taaggccgga catgtgtcgc
      541 ttcgcagggc gttgtatatg cccgcaatgg tagccaccag taagactgag tggggacggg
      601 cgttccgcga ccgtctggcg gctaatggca agaaaggaaa ggtgattctc ggcgcgatga
      661 tgcgcaagct ggcacaggtg gcgtatggag tgctgaagtc aggcgtgccg ttcgatgcgt
      721 cacggcataa tccggtagcg gcgtaaaaat cgcggaaggg atgaaaaaaa cagcgcctga
      781 cggcgctgtg tctggcatgc ctgcaatccg ggaaaccgga ccaggaaaaa actt
//
LOCUS       DAZFWL020000484          826 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFWL020000484 DAZFWL020000000
VERSION     DAZFWL020000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.490"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            189..647
                     /locus_tag="ACHKNP_RS27965"
                     /old_locus_tag="pgaptmp_005593"
     CDS             189..647
                     /locus_tag="ACHKNP_RS27965"
                     /old_locus_tag="pgaptmp_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707509.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
     gene            747..826
                     /locus_tag="ACHKNP_RS27970"
                     /old_locus_tag="pgaptmp_005594"
                     /pseudo
     CDS             747..826
                     /locus_tag="ACHKNP_RS27970"
                     /old_locus_tag="pgaptmp_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT          279 a          139 c          137 g          271 t
ORIGIN      
        1 ttaaatcaat cacctgatta aaccatgaaa gtgtgatgta agtcaaaatt actgatacta
       61 tcaattgata atgattatct ctatcattat cgtgaaattc gagaatgaaa ggagaagtag
      121 tcattataag ccttttataa atcgtcatca tcatagtaaa gtagaacact ttttatatga
      181 gttactatat gaaaatatac tggacaagaa aacgtattcc tgaactttca gcacttccac
      241 cttcattaag aaagaaaaat tttactgacg catataatgc agcatcttct catatcgaat
      301 actggatagg tgctggcgtc tcatttatct cgatgatgat tttatccaga gtatatgact
      361 ttcttttacc cgcccaagat actttcccgg gtgatatcat cagaagttta tgtgttgtgt
      421 gtccatccat attaatctgg tttcagtttt cggtatatgt catgaggaaa tattacagac
      481 atattctggt gcgaggaaaa gaaaccgaaa ccatttcaga aaggttgata cgtgaggctg
      541 atacacgaga atatgaacta tggcgtccgg tccgtcgctt tttctccatt gttttcctca
      601 ttgttctatt aggttgcatt cattcactga taacaactat aaaataaaag ttatggctgt
      661 tctgatacag gaaaataaac gttcattcta tggttacctc ttcctgttat atagaagaat
      721 tttaaacgac aagtaaggga atataaatga aaataacaaa aaataaaaat gcaactataa
      781 aaaacagtca tcagtcggct gcttttcaat gtttacctta tcattt
//
LOCUS       DAZFWL020000485          825 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFWL020000485 DAZFWL020000000
VERSION     DAZFWL020000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.491"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..299
                     /locus_tag="ACHKNP_RS27975"
                     /old_locus_tag="pgaptmp_005595"
     CDS             <1..299
                     /locus_tag="ACHKNP_RS27975"
                     /old_locus_tag="pgaptmp_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system filament chaperone"
                     /protein_id="HGU4707510.1"
                     /translation="RELLDKKIRSEIEVIKKIIAEFDVVKENVNALSEKAKTDPQAAE
                     KLNKLIEGYTYGEERKLYDSALSKIEKLIETMSPPRSKNQSTKNQRNKNNRKIV"
     gene            296..>825
                     /locus_tag="ACHKNP_RS27980"
                     /old_locus_tag="pgaptmp_005596"
     CDS             296..>825
                     /locus_tag="ACHKNP_RS27980"
                     /old_locus_tag="pgaptmp_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system domain-containing
                     protein"
                     /protein_id="HGU4707511.1"
                     /translation="MTILNKIDYNYYKLINYPIMMVHDEWLGDLTGVNQVSFRHLRES
                     SSTRNQLNKILRQEIQDKISGVELSDINKEGFLYQSIGKIRLLALSSALFEIQCPDYI
                     FSRLYRETLIREIGYQNVKQLSFYWQGGQCKPEYGEERFCSELIKYGAGNLEWLFSDN
                     PLWTIVKYLLPKSGEI"
BASE COUNT          332 a          102 c          156 g          235 t
ORIGIN      
        1 atagagaact gttagataaa aaaataagat cagaaattga ggtgataaaa aaaataatcg
       61 ccgaatttga tgtcgtaaaa gaaaacgtta atgctttaag tgaaaaagca aaaacagatc
      121 ctcaggctgc agaaaaatta aacaaattaa ttgaggggta tacatatggt gaagagcgaa
      181 aattatatga ttctgcctta agtaagattg aaaaactaat agagacaatg agtcctccaa
      241 gatctaaaaa ccaatcaaca aaaaatcaac ggaataaaaa taatagaaaa atagtatgac
      301 gattttgaat aaaatagact ataactatta taaattaatt aattatccaa ttatgatggt
      361 ccatgatgaa tggctaggtg atttaaccgg tgtcaatcag gttagttttc ggcatttaag
      421 agaaagtagt tctactcgta atcaactcaa caagatattg cgtcaggaaa tacaagataa
      481 aatttctgga gtggaattat ctgatataaa taaggaaggt tttttatatc agtcaattgg
      541 gaaaataaga ttgcttgctc taagtagtgc attatttgaa attcaatgcc ctgactatat
      601 tttttcacga ttatatcgag aaactctgat cagagagata ggatatcaaa atgtgaagca
      661 gttatcattc tattggcagg gagggcaatg taaaccagaa tatggcgaag aacggttttg
      721 ttctgaactg ataaaatacg gtgcaggaaa tcttgaatgg ctattttctg ataatccctt
      781 atggactata gtcaaatatc ttttaccgaa aagtggagaa ataaa
//
LOCUS       DAZFWL020000486          819 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFWL020000486 DAZFWL020000000
VERSION     DAZFWL020000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.492"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..723
                     /locus_tag="ACHKNP_RS27985"
                     /old_locus_tag="pgaptmp_005597"
     CDS             <1..723
                     /locus_tag="ACHKNP_RS27985"
                     /old_locus_tag="pgaptmp_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGU4707512.1"
                     /translation="PQEYSLSFIKQLPNTVNLSSLTYNKEDDFLYATQNSPAQLLKIA
                     RSGDIIGRAPLPFISDAETIEHIQGNIFAAVDEKTSELFFFTVTKDMHISLRNKIQLE
                     KFNKKNRGFEGLAWKAEGRMLFVAKERRPTGMFAYQLSPDLLSAKQVTIPEELNDIHV
                     KDISGLDFNNESLMILSDESRKLLKFNLTEMSFVEMMDLTKGNHSLTSDLLQPEGIVT
                     LSDESIYVASEPDILAKFVPNK"
BASE COUNT          272 a          168 c          152 g          227 t
ORIGIN      
        1 ccacaggaat actcactttc atttataaaa caattaccaa atacggtgaa cttatcatct
       61 cttacgtata ataaagagga tgatttcctg tatgccaccc agaatagccc tgcgcagcta
      121 cttaaaatag ccagaagtgg cgacatcatc ggccgagccc cgctgccttt tatctctgat
      181 gccgaaacca ttgagcacat acaagggaac atttttgctg cagtagacga aaagacttct
      241 gaactattct tttttacagt tacaaaagat atgcatatct ccttgcgaaa taaaattcag
      301 ttagaaaaat tcaataaaaa aaatagaggg tttgaaggac tggcatggaa agcagaaggc
      361 cggatgctgt ttgtcgctaa agaacgacgc cctacaggca tgtttgccta tcagctttct
      421 ccagatctgc tgagtgcaaa acaagtaacg attccagagg aactaaatga tatacatgtt
      481 aaagatatat ccggcctgga ctttaataat gaaagtctga tgattctgtc cgatgaatcg
      541 cgtaagttac tcaagtttaa cctgacagaa atgtctttcg ttgagatgat ggacctgaca
      601 aaaggaaatc attctctgac cagtgatttg ctccaacctg aaggcattgt aactctgtct
      661 gatgaaagca tttatgtcgc tagtgaacct gatatcctcg ctaaatttgt ccctaataaa
      721 taagtctgtc actagcctta gtattgagcg accaaatctg gcaatctcat gaaactcgca
      781 aaatacgaat ctaatattaa taaaaatttt actctgaaa
//
LOCUS       DAZFWL020000487          817 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFWL020000487 DAZFWL020000000
VERSION     DAZFWL020000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.493"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>817
                     /locus_tag="ACHKNP_RS27990"
                     /old_locus_tag="pgaptmp_005598"
     CDS             <1..>817
                     /locus_tag="ACHKNP_RS27990"
                     /old_locus_tag="pgaptmp_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4707513.1"
                     /translation="KALALGATEITKEEYFARKNGTYSGSQQNTGASDTTAQPEPVKV
                     TADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRDSVNQNQQE
                     SEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPDCGAVMGDA
                     TYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGETADHSIKVNGH
                     QEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLE"
BASE COUNT          281 a          196 c          206 g          134 t
ORIGIN      
        1 aaagcactag ccttgggagc tacggaaatc acaaaagagg aatacttcgc acgtaaaaac
       61 ggcacgtact caggttcaca acaaaatact ggtgcatctg acacgaccgc acaaccagag
      121 ccggtaaaag ttaccgctga cgaagtaaac aaaattatgc aggcagccaa tatcagccag
      181 cctgacgccg ataagttgct tgctgcctct cgcggagaat ttgttgcagg gattagcgac
      241 ccgaatgatc cgaaatgggt aaaggggatt gaaacccgcg attctgtaaa ccagaaccag
      301 caagaatcgg aacagaacga ccagaaagcg gaacaaaaca gcccaaatac gcaacaaaac
      361 gagccagaaa cgaaacaacc tgagccagta gcgcaacagg aaccggaaaa agtctgcacc
      421 gcctgcggtc agaccggcgg cggcaactgc cctgattgtg gcgcggtgat gggcgacgca
      481 acataccagg aaacattcga tgaagagtat caggttgaag ttcaggaaga tgatccggag
      541 gaaatggaag gcgctgaaca tccacacaag gagaacactg acggcaatca gcatcacgat
      601 agcgataatg aaactggcga gacggcagat cactcaatta aggtgaacgg tcatcaagaa
      661 atcacatcca ccagcaggac gtgtgaccat ctaatgatcg accttgaaac catgggaaaa
      721 aatcctgatg ccccgatcat ctcaataggt gcaatatttt tcgatccgca aaccggagat
      781 atggggccgg aatttagtaa gactatcgat ctggaaa
//
LOCUS       DAZFWL020000488          785 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFWL020000488 DAZFWL020000000
VERSION     DAZFWL020000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.494"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..321
                     /locus_tag="ACHKNP_RS27995"
                     /old_locus_tag="pgaptmp_005599"
     CDS             <1..321
                     /locus_tag="ACHKNP_RS27995"
                     /old_locus_tag="pgaptmp_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4707514.1"
                     /translation="REFGECKGKKHYHLMLLVNRDTWCRAGDYRAPGSLAGMIKQAWC
                     SALGVDVGCHATLVHFPAWPAVWLARNDDTGFQQVLERADYLAKEHTKVHCTGERNFG
                     CSRG"
     gene            487..>785
                     /locus_tag="ACHKNP_RS28000"
                     /old_locus_tag="pgaptmp_005600"
     CDS             487..>785
                     /locus_tag="ACHKNP_RS28000"
                     /old_locus_tag="pgaptmp_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4707515.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHC"
BASE COUNT          174 a          215 c          226 g          170 t
ORIGIN      
        1 cgggagtttg gggagtgcaa aggtaaaaaa cactatcacc tgatgctgct ggtcaaccgg
       61 gatacctggt gtcgtgccgg tgattaccgc gctccgggat cactggccgg gatgattaaa
      121 caggcgtggt gcagtgccct gggagtggat gtcgggtgcc atgccacgct ggtgcatttt
      181 ccggcctggc cggcggtgtg gctggcgcgt aatgatgaca ccggctttca gcaagtgctg
      241 gaacgtgctg actatctggc gaaggagcat accaaagttc actgcaccgg tgagcgcaac
      301 tttggctgca gtcgtggctg agccagacag acgagttatc tgacttcagt gtgatgacgt
      361 gttcacggcc accacactga ttttccctta tgagactgaa ctgaaaccgc cattcacacc
      421 ggaccacgct gcccgtaccg ggacggctgt tgcctgcgtg tcgtctgacg gtaaggaaca
      481 tatactatgt acgcaaaatc ctttctcgct cttgatggca acggacgtct gacgggcgcc
      541 cgtacagcac aggccgcacc ttatgctcac tacacctgcc acttgtgtgg cagtgcactc
      601 agataccatc cgcaatacga cactgaactt ccctggtttg aacacactga cgacaggctg
      661 acagagcacg gtcaacagtg cccttatgtc aggccggagc gcagagaaat acagttgatt
      721 aaacgtctgc agcaattcgt accggatgcc ttacccgtgg tgcgtaaagc cagctggcac
      781 tgcag
//
LOCUS       DAZFWL020000489          779 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFWL020000489 DAZFWL020000000
VERSION     DAZFWL020000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.495"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..136)
                     /locus_tag="ACHKNP_RS28005"
                     /old_locus_tag="pgaptmp_005601"
     CDS             complement(<1..136)
                     /locus_tag="ACHKNP_RS28005"
                     /old_locus_tag="pgaptmp_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4707516.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     A"
     gene            525..719
                     /locus_tag="ACHKNP_RS28010"
                     /old_locus_tag="pgaptmp_005602"
     CDS             525..719
                     /locus_tag="ACHKNP_RS28010"
                     /old_locus_tag="pgaptmp_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4707517.1"
                     /translation="MSTKNKTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDE"
BASE COUNT          256 a          201 c          152 g          170 t
ORIGIN      
        1 cggcggcaga gtcataaagc acttcattac ccttgccacc accccgcaga acgggcattc
       61 cctgctcctg ccagttctga atggtacgga tactcgcgcc gaaaatgtca gccagctgct
      121 ttttgttgac ttccattgtt cattccacgg acaaaaacag agaaaggaaa cgacagaggc
      181 caaaaagctc gttttcagca cctgtcgttt cctttctttt cagagggtgt tttaaataaa
      241 aacattaagt tacgacgaag aagaacggaa acgccttaaa ccggaaaatt ttcataaata
      301 gcgaaaaccc gcgaggtcgc cgccccgtaa cctgtcggat cgccggaaag gacccgcaaa
      361 atgataataa ttatcatcta tatgtcacaa cgtgcatcta cgccatcaaa ccacgtcaaa
      421 taatcaatta tgacgcaggt atcgtattaa ttgatctgca tcaacttaac gtaaaaacaa
      481 cttcagacaa tacaaatcag cgacactgaa tacggggcaa cctcatgtca acgaagaaca
      541 aaacccgcag aacaacaacc cgcaacatcc gctttcctaa ccaaatgatt gaacaaatta
      601 acatcgctct tgagcaaaaa gggtctggga atttctcagc ctgggtcatt gaagcctgcc
      661 gtcggagact aacgtcagaa aagagagcat atacatcaat ccaaagtgat gatgaataaa
      721 catcccggtt tcttccacca tcgcaccgga aaagcgacta tgagggtaac cctgcgtct
//
LOCUS       DAZFWL020000490          773 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFWL020000490 DAZFWL020000000
VERSION     DAZFWL020000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.496"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            167..733
                     /gene="slp"
                     /locus_tag="ACHKNP_RS28015"
                     /old_locus_tag="pgaptmp_005603"
     CDS             167..733
                     /gene="slp"
                     /locus_tag="ACHKNP_RS28015"
                     /old_locus_tag="pgaptmp_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HGU4707518.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          235 a          167 c          168 g          203 t
ORIGIN      
        1 atggatactc atgccacgcc ccaaaatcaa tggaattgaa ctaaacaaaa gggatataaa
       61 catcagacag gtttacgtta ctatcaggca tatcacctca gaatcagatg aaaactataa
      121 agaaatatct attatggctt taatatttgt tgataaggat agtaacatga acatgacaaa
      181 aggtgcactc atcctcagcc tttcattttt gcttgccgca tgtagttcaa ttccgcaaaa
      241 tatcaaaggc aataaccaac ctgatattca aaaaagtttt gttgctgttc ataaccagcc
      301 ggggttatat gttggtcaac aagcgcgctt tggtgggaag gttatcaacg ttatcaatgg
      361 caaaacggat acgttgttag aaatcgctgt attaccgttg gatagctatg cgaagcctga
      421 tattgaagcc aactatcagg gccgactgct cgccagacaa agcggcttcc ttgatccagt
      481 gaactatcgt aatcactttg ttaccatcct cggtaccatt cagggtgaac aaccaggctt
      541 tatcaataaa gtcccgtata acttcctgga agtgaatatg cagggcatcc aggtgtggca
      601 tttgagagaa gtggttaata ccacctataa cctgtgggat tacggctatg gtgcattctg
      661 gccggaaccg ggctggggtg cgccttacta caccaatgcg gtgagtcagg taacacctga
      721 gctggtcaaa taacaccacc gaaagatgca gatgctttct cagcatctgc atc
//
LOCUS       DAZFWL020000491          771 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFWL020000491 DAZFWL020000000
VERSION     DAZFWL020000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.497"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            84..>771
                     /locus_tag="ACHKNP_RS28020"
                     /old_locus_tag="pgaptmp_005604"
     CDS             84..>771
                     /locus_tag="ACHKNP_RS28020"
                     /old_locus_tag="pgaptmp_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4707519.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQ"
BASE COUNT          257 a          131 c          168 g          215 t
ORIGIN      
        1 tcctgttatg atataatatg ccgaattaac acatgttaac gatttaccag taatgtaaat
       61 aaattttcga ggagatcatt ccagtgggac gtaaatgggc caatattgtt gctaaaaaaa
      121 cggctaaaga cggtgcaacg tctaaaattt atgcaaaatt cggtgtagaa atctatgctg
      181 ctgccaaaca aggtgaaccc gatccagaat taaacacatc tttaaaattc gttattgaac
      241 gtgcaaagca ggcacaagtt ccaaagcacg ttattgataa agcaattgat aaagccaaag
      301 gcggcggaga tgaaacgttc gtacagggac gttatgaagg ctttggtcct aatggctcaa
      361 tgattattgc tgagacattg acttcaaatg ttaaccgtac gattgctaac gttcgcacaa
      421 ttttcaataa aaaaggcggc aatatcggag cggcaggttc tgtcagctat atgtttgaca
      481 atacgggtgt gattgtattt aaaggaacag accctgacca tatttttgaa attttgcttg
      541 aagctgaagt tgatgttcgt gatgtaactg aagaagaagg taatattgtt atttatactg
      601 aacctactga ccttcataaa ggaatcgcgg cgctaaaagc agctggaatc actgagttct
      661 caacaacaga attagaaatg attgctcaat ctgaagttga gctttcccca gaagatttag
      721 aaatctttga agggcttgtt gatgcccttg aagatgacga cgatgtacaa a
//
LOCUS       DAZFWL020000492          770 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFWL020000492 DAZFWL020000000
VERSION     DAZFWL020000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.498"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            44..634
                     /locus_tag="ACHKNP_RS28025"
                     /old_locus_tag="pgaptmp_005605"
     CDS             44..634
                     /locus_tag="ACHKNP_RS28025"
                     /old_locus_tag="pgaptmp_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4707520.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          239 a          162 c          173 g          196 t
ORIGIN      
        1 gatgcattat gatatgaaca ccaatttcgt atagagtctc actatgtctc gaatttttgc
       61 ttactgtcgg atatcaacgc tggatcagac catcgaaaat caacgccggg aaatcgaaag
      121 tgcaggtttt aaaatcaaac ctcagcaaat aatcgaagaa cacattagcg gctcagcagc
      181 aaccagtgag cgtcctggtt ttaaccggtt gcttgctcgc ctgaaatgtg gtgatcaatt
      241 gattgtgaca aaactggatc gccttggttg taatgcaatg gatatcagga aaacagtgga
      301 acaactgacc gaaacaggta tcagagtgca ttgcttagca ttggggggca ttgacctgac
      361 cagtccaaca ggaaaaatga tgatgcacgt aatttcagca gtcgctgaat ttgaacgaga
      421 ccttttactt gaacgcactc attccgggat agtaagagcc cgcggcgcag ggaaacgttt
      481 tggtcgacca cctgtgttaa atgaagaaca gaaacaggtg gtattcgaac gaattaagtc
      541 aggtgtaagt ataagtgcca ttgcccggga attcaaaacc tcgcggcaaa ccattttaag
      601 agccaaagca aaacttcaga cacctgacat ataaaaaata atctcggtgt gagatgcttt
      661 acgtcttcca agcccccttc cttgccgtaa atggaaagat acatctaatt atagaattta
      721 tatgttttac cctacggcag tgctggccat tcaatatcct gtgcagttga
//
LOCUS       DAZFWL020000493          770 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFWL020000493 DAZFWL020000000
VERSION     DAZFWL020000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.499"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            76..690
                     /gene="espF"
                     /locus_tag="ACHKNP_RS28030"
                     /old_locus_tag="pgaptmp_005606"
                     /pseudo
     CDS             76..690
                     /gene="espF"
                     /locus_tag="ACHKNP_RS28030"
                     /old_locus_tag="pgaptmp_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
BASE COUNT          206 a          190 c          165 g          209 t
ORIGIN      
        1 tagccgttaa ctatttttaa ttttaataca aattaataat tattcctgtt gattagatat
       61 aaaaaggcat gaattatgct taatggaatt agtcaagctg tttctacact aggacggcat
      121 attactagtg cggtaagccg ggtaagctct gcggggttat ctggcttttc agtgtcccct
      181 caggctgttc gtctgaatcc gattagggct caatctcctt tttctcccgg tacgtcgaat
      241 atcaatgcga gaacgacttt taatgtgagt agcccggcga cttcatttac tccctctcgt
      301 ccggccccac cgccgccgac aagtggacag gcttccgggg catcccgccc tttaccgccc
      361 attgctcagg cattaaaaga tcatttagct gcctatgaaa agtcgaaaag tcttgatact
      421 tcaagcctta agccaagccg cccggcaccg ccgccgccga caagcgggtt ggtatctggg
      481 tcatcccggt ctttaccgcc cattgctcag gcgctaaaag atcatttggc tgcctatgaa
      541 aaatcgaaaa gtcttgatac ttcaggtctt aagccaagcc gcccggcacc gccgccaccg
      601 acaagtggac aggcatccga ggcatcccgg ccattaccgc ctattgctca ggcattaaaa
      661 gatcatttag ctgcctatga actgtcgaaa aaggcataaa aagtagattt aaaattatta
      721 attactgttg attgtttaga gagttaaaag ataaaaaccg atattaagtt
//
LOCUS       DAZFWL020000494          763 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFWL020000494 DAZFWL020000000
VERSION     DAZFWL020000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.500"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..307)
                     /locus_tag="ACHKNP_RS28035"
                     /old_locus_tag="pgaptmp_005607"
     CDS             complement(<1..307)
                     /locus_tag="ACHKNP_RS28035"
                     /old_locus_tag="pgaptmp_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HGU4707521.1"
                     /translation="MATLRELIIKISANSQSFQSELQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     "
     gene            complement(358..567)
                     /locus_tag="ACHKNP_RS28040"
                     /old_locus_tag="pgaptmp_005608"
     CDS             complement(358..567)
                     /locus_tag="ACHKNP_RS28040"
                     /old_locus_tag="pgaptmp_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HGU4707522.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVSLEDAMLCW
                     GEDEDKVESDPFAALEDALSFAAMS"
     gene            complement(663..>763)
                     /locus_tag="ACHKNP_RS28045"
                     /old_locus_tag="pgaptmp_005609"
     CDS             complement(663..>763)
                     /locus_tag="ACHKNP_RS28045"
                     /old_locus_tag="pgaptmp_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU4707523.1"
                     /translation="REQVLAVYGPVHARLLRQALELIADAESARKK"
BASE COUNT          166 a          252 c          186 g          159 t
ORIGIN      
        1 gcgacgcctg tttcagacgg gcattcacgg aactccactc atcagccagt gaaatcaggt
       61 gtccggtggc aaaggcaccg gcaaatgcgc cagccattcc gacagcagaa ccgcgaattt
      121 ccgtcaactg gctgttcagt tctgccaggg cacgtcgctg ctcccgggct gccgcagcgg
      181 cctgacgccc gccattctgc agggtccggt aatattcact gcccatacgg gaagcacgct
      241 ggagctccga ctggaatgac tgtgaatttg ccgaaatttt gataatcagt tcacgtaacg
      301 tcgccattca cctttctccg ggcaaaaaaa cctgccacag caggttttca tcattattta
      361 tgacattgct gcaaagctca gcgcgtcttc cagcgccgca aacggatctg actctacttt
      421 atcctcatcc tcgccccagc agagcatggc gtcctccagt gaaacattca tcccctgcgc
      481 cccgaaaacc gctttcacga tctgcgcatt acggatatcc ccgcgctcat cgcccagcgg
      541 ggacaccctg tcgaactcca tccacatcat cgcctcgctc gcactcagac tgtggcccag
      601 ttcggataag gtgcgcccca gacggagcgc aagtcgcatc agaaagcgaa tttccgggcg
      661 ggctactttt ttctggccga ctctgcatca gcgatcagtt ccagtgcctg acgcagcaac
      721 cgggcatgta ccggaccata gacggccagc acctgctcac ggt
//
LOCUS       DAZFWL020000495          763 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFWL020000495 DAZFWL020000000
VERSION     DAZFWL020000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.501"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>763
                     /locus_tag="ACHKNP_RS28050"
                     /old_locus_tag="pgaptmp_005610"
     CDS             <1..>763
                     /locus_tag="ACHKNP_RS28050"
                     /old_locus_tag="pgaptmp_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707524.1"
                     /translation="LISEREVSEYECQLLYLTNPIDVMNILNKYIYYFPNENSPFYTL
                     KIDSALSWDAIDAIRDYSISDIYLYPEQKTINCVVEIENIVFGGYIYTLNNGVTLQNI
                     ENTLKDSSCHYVLNGYTEFVNCLRQLTSGKTESVHRTINKLNYEKLPFGFIIAAFAIL
                     KIAFKIKFSKNHVNIRALLNDINYFMTYQGESINLISLDHEYPESCLQNDTNTYLLGR
                     VIFLYNSMIYKFINCQEHETNNIHSAMINNLLQEVD"
BASE COUNT          284 a          128 c          112 g          239 t
ORIGIN      
        1 gctgataagc gaaagagaag tttcagaata tgaatgccag ttattatatt taaccaaccc
       61 aatagatgta atgaatatac tgaacaaata catatattac tttcctaatg agaactcacc
      121 attttataca ctgaaaattg acagtgcatt atcgtgggat gccattgacg caatacgaga
      181 ctatagtatt tctgatattt atctttatcc tgaacaaaaa acaataaatt gtgtcgttga
      241 gattgaaaac attgtctttg gcggttacat ttatacattg aacaacggcg tcacattaca
      301 aaacatagaa aacactttaa aagattcttc atgccattat gtcttaaatg gctatacaga
      361 atttgttaac tgtttgagac aacttacttc aggaaagact gaaagtgttc atcgcaccat
      421 caataaactg aactatgaga aattaccttt tggatttatc attgccgcgt ttgctatact
      481 aaagatagca tttaaaataa aattcagtaa aaatcatgta aatatccgag cattattaaa
      541 tgacatcaat tattttatga cttatcaggg cgagtccatt aaccttattt cactggatca
      601 cgaataccca gagtcctgtc ttcaaaatga cacaaacaca tatttattag gaagagtaat
      661 atttctgtat aactcaatga tttataagtt cataaactgt caggaacatg aaaccaataa
      721 cattcactca gctatgataa ataacctatt acaggaagtt gat
//
LOCUS       DAZFWL020000496          761 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFWL020000496 DAZFWL020000000
VERSION     DAZFWL020000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.502"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>761
                     /locus_tag="ACHKNP_RS28055"
                     /old_locus_tag="pgaptmp_005611"
     CDS             <1..>761
                     /locus_tag="ACHKNP_RS28055"
                     /old_locus_tag="pgaptmp_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4707525.1"
                     /translation="LIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVYTTTQSNEPP
                     AGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAMVNPNLGYSV
                     DEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQQGRRVSLDD
                     ILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVVRRKSEASRF
                     QDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELL"
BASE COUNT          153 a          172 c          266 g          170 t
ORIGIN      
        1 ctgattgatg aactgtggct gtttggcaag cagtacaagg cggaagacat gctacgtgaa
       61 gccatcggcg ggcttgcctc ccgtccggaa gggtttgtgg tgtatacgac cacccagtcg
      121 aatgagccgc cagccggggt gttcagacag aaactgcagt acgcccggga tgtccgtgac
      181 ggcaaaattc atgatccgca ttttctgccg gtgatatttg aacaccctcc tgaaatggtg
      241 gaaagcgggg ctcacctgct gatggaaaac ctcgccatgg tcaatccgaa tctcggttat
      301 tcggtggatg aggcttttct gtaccgggag taccgtaaag cccgggaggc tggtgaggaa
      361 gcatttcgtg gcttcatgtc aaaacatgcc aatgtggaaa ttggtcttgc cctgcgttct
      421 gaccgctggg cgggtgcgga tttctgggag cagcagggca ggcgcgtcag cctggacgat
      481 atcctgcagc gcgctgatgt ggtgacggtg gggattgacg gcgggggcct ggatgatctg
      541 ctgggaatgt acgtgattgg ccgtgacagg gaaacccgcg aatggctggg ctggggccat
      601 gcctgggcgc atgaaaccgc ggtggtcaga cggaagagtg aggcatcccg ttttcaggat
      661 tttgtggcct gtggagacat gacgattgtc cgtcgggtcg gggatgacac ggcggaagtg
      721 gcggagtatg tgcgtcgtat tcatgaggct gagttactgg a
//
LOCUS       DAZFWL020000497          759 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFWL020000497 DAZFWL020000000
VERSION     DAZFWL020000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.503"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..276
                     /gene="yddL"
                     /locus_tag="ACHKNP_RS28060"
                     /old_locus_tag="pgaptmp_005612"
                     /pseudo
     CDS             1..276
                     /gene="yddL"
                     /locus_tag="ACHKNP_RS28060"
                     /old_locus_tag="pgaptmp_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            315..>759
                     /locus_tag="ACHKNP_RS28065"
                     /old_locus_tag="pgaptmp_005613"
     CDS             315..>759
                     /locus_tag="ACHKNP_RS28065"
                     /old_locus_tag="pgaptmp_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGU4707526.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQ"
BASE COUNT          268 a          155 c          129 g          207 t
ORIGIN      
        1 atgaaattaa aaatagttgc ggtggttgta actggtttgt tagctgcgaa cgtagcacac
       61 gctgccgaag tctataacaa ggatggtaat aaactcgacc tttatggcaa ggttaccgct
      121 ctacgttatt ttactgatga taagcgtgac gatggtgata aaacttatgc ccgtctcggc
      181 tttaaaggag aaacgcaaat caatgatcaa atgattggtt ttggtcactg ggaatatgat
      241 tttaaaggct ataacgatga agccaacggc tcgcgcggca agaatctctg attgagttaa
      301 gtgcccatca caatatgatc actgacctta tattacacaa tcatcccagg atgaaaacaa
      361 tcactttaaa cgacaaccat attgcacatt taaacgccaa aaacactaca aaactggaat
      421 atttaaactt aagcaataac aatttactgc caaccaatga cattgatcaa ctaatatcat
      481 caaaacatct ttggcatgta ttagttaacg tcatcaacaa tgatccactt gcacaaatgc
      541 agtactggac tgcagtaaga aatataattg atgacactaa tgaagtgacc attgatttat
      601 caggacttaa tttaaccact caaccaccag ggctgcaaaa cttcacctct atcaatcttg
      661 ataataacca actcacacat tttgatgcaa ccaactacga tagactcgta aagctaagtc
      721 tgaatagtaa tgctcttgag tcaataaatt ttcctcaag
//
LOCUS       DAZFWL020000498          752 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFWL020000498 DAZFWL020000000
VERSION     DAZFWL020000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.504"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            79..752
                     /locus_tag="ACHKNP_RS28070"
                     /old_locus_tag="pgaptmp_005614"
                     /pseudo
     CDS             79..752
                     /locus_tag="ACHKNP_RS28070"
                     /old_locus_tag="pgaptmp_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; incomplete;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          218 a          181 c          163 g          190 t
ORIGIN      
        1 acagattatg tcagaaggcc ccggtgatgc cttctgacga tagattaatg ttgtattcta
       61 ttggtatctg gagaccatat ggcaacagtt acagtatatt ctccccgctg taattcctat
      121 aaagtgtacc gttatgatca cagcacctca gatcatgagc gtttccagtg ctaatcctgc
      181 aaacgtgtat ttcaactgaa ttacatctac gaaacccgaa aaccaggcat taaagagcaa
      241 attgttgaaa tggttcataa cggagccggg gttcgcgata gctccagaac gctgaaggtg
      301 gatatcaaca ccgtcattct cactttaaaa aacgctcacc acgtaaagta accatatctc
      361 cagccgccag tgtcgatgtg gcacttatct gtgaacttga tgaacaatgg agttttgtcg
      421 aaaacaaagc tcgtcagcag tggcactggt acgcgtataa gaccaaagct gacggtgtgc
      481 tggcttacac ttttggtcct cgcactgatg aaacatgccg tgagttgccg gaattcctga
      541 agccattctc cgcaggcatg ataaccagag acaaccggag cagttatacc agagaaatgc
      601 cacaggataa gcatcttgtc ggcaagatat ttacccggcg cattgagcgc aacaatctga
      661 cacttcgtac tcacatcaaa cgacctgccc ggaaaacgat ttgcttttta cgctccctgg
      721 aaattcacga aaagccattg gttcatttat cg
//
LOCUS       DAZFWL020000499          749 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFWL020000499 DAZFWL020000000
VERSION     DAZFWL020000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.505"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>749
                     /gene="yfdF"
                     /locus_tag="ACHKNP_RS28075"
                     /old_locus_tag="pgaptmp_005615"
     CDS             <1..>749
                     /gene="yfdF"
                     /locus_tag="ACHKNP_RS28075"
                     /old_locus_tag="pgaptmp_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HGU4707527.1"
                     /translation="FNGKEGISTCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIG
                     SNDTDHITIGIDNQEPIELPANYKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIY
                     DGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDY
                     LSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARNASAWFESSKAIMAESFATGSDHQV
                     VNELNGERLREPNEVFKRLGRAIRYNFQVDD"
BASE COUNT          276 a          129 c          144 g          200 t
ORIGIN      
        1 tttttaatgg taaggaagga ataagcacct gtattaaaca tctacttgag cttataaaaa
       61 acgccatacg agtaaacgac gatccttata gatttaatat taataattcc tcagttactt
      121 atattgatat tggctccaat gatacagacc atattactat tggtatcgac aaccaagaac
      181 caatagaatt acctgcgaac tataaagaca aagaactcgt ccgtactatc attaatgaca
      241 acatagttga gaagactcat gatatcaata acaaggaaat gatcttcagc gcattaaaag
      301 aaatatatga tggagatcct ggttttatct tcgataagat atcacacaaa ctcagacata
      361 cggtaacgga gtttgatgag agcgggaata gcgaaccaac ggacttattt acctggtacg
      421 gtaaagataa aaaaggcgac tctctcgcta ttgtaattaa aaataaaaac ggaaatgatt
      481 acttatctct cggttactac gatcaggacg actaccacat tcaaagagga attcgtatta
      541 atggtgatag tctcacccaa tattgtagtg aaaaagccag gaatgcttca gcgtggtttg
      601 aaagcagtaa agctatcatg gcagaatcat ttgcaacagg ttccgatcat caggttgtaa
      661 acgagctcaa cggggaaaga ctgagagaac ctaacgaagt ttttaaacgt ttaggtcgag
      721 caataagata taattttcaa gtggacgat
//
LOCUS       DAZFWL020000500          747 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFWL020000500 DAZFWL020000000
VERSION     DAZFWL020000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.506"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..663
                     /locus_tag="ACHKNP_RS28080"
                     /old_locus_tag="pgaptmp_005616"
     CDS             <1..663
                     /locus_tag="ACHKNP_RS28080"
                     /old_locus_tag="pgaptmp_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4707528.1"
                     /translation="NLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYI
                     TQDPIGLKGGWNLYTYPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKE
                     YGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVT
                     KENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQG
                     SN"
     gene            665..>747
                     /locus_tag="ACHKNP_RS28085"
                     /old_locus_tag="pgaptmp_005617"
     CDS             665..>747
                     /locus_tag="ACHKNP_RS28085"
                     /old_locus_tag="pgaptmp_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HGU4707529.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNI"
BASE COUNT          263 a          117 c          169 g          198 t
ORIGIN      
        1 aacctgctga atgaagagaa cccgcatcag ctgcagcagc ttatccgcct gccggggcag
       61 cagtatgatg aggagtccgg cctgtattac aaccgccacc gctattatga cccgctgcag
      121 gggcggtata tcactcagga tccgattggg ctgaaggggg gatggaacct gtatacatat
      181 ccgctgagcc cggtgaatag catggatcca ttaggattat atgaatttaa atcaaaaaat
      241 atagatgata ttggaatatt tgcattggca atgtgtaatg gagaatcaat taacgagaat
      301 aaagaatatg gtggactaat atgtaagaag caaggtgaat atttccccat gaatccgata
      361 agttcaaatg ataatgatag tgtagacttg cgaaatataa aatgccctga aggttcagag
      421 agagtaggcg attatcacac tcacggtttt tactctgacg ataaaggaaa taaagtaaca
      481 aaagaaaatg atgtttatga tagtctaaat ttttcaagca aagatttaac gaattcttat
      541 atgaatggaa tgggaaaaaa agaatacagt agttacttgg gaacaccaaa taacacctat
      601 ctaaaatata atcccaaagc taaagggaat ggagttacaa ttatcaggca agggagtaat
      661 taaaatgaag gcaatacaat gttgttattt ccttgcttta gtctccagtc atttaatggc
      721 tggagttatg ggtgatgata atatatt
//
LOCUS       DAZFWL020000501          744 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFWL020000501 DAZFWL020000000
VERSION     DAZFWL020000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.507"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>744
                     /locus_tag="ACHKNP_RS28090"
                     /old_locus_tag="pgaptmp_005618"
     CDS             <1..>744
                     /locus_tag="ACHKNP_RS28090"
                     /old_locus_tag="pgaptmp_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707530.1"
                     /translation="KGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQSIL
                     VNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTRTI
                     TSANIDRLRVTFGVQSLVQTTSKGDRNPTSVRLLIQLQRNGNWVTEKDVTINGKTTSQ
                     FLASVILDNLPPRPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIVGL
                     QVDAEQFGGQQMTVNYHIRGRIIQVPSNYD"
BASE COUNT          181 a          200 c          232 g          131 t
ORIGIN      
        1 aaaggtggcg gcagggcgca cacacctcgt gaggcgaagg acaatctcaa atccacgcag
       61 atgatgagcg tgattgacgc cattggtgaa gggccgattg aaggtccggt gaaggggctg
      121 cagagtatcc tggtgaacaa aaccccgctg acggacacgg acggtaatcc tgtgatacat
      181 ggtgtgaccg cggtctggcg cgccggggag caggagcaga caccacctga aggctttgag
      241 tcctccgggg cggaaaccgc actgggcgtg gaagtgacga aggcaaagcc ggtgacgcgc
      301 accattacat ccgcgaacat tgaccgcctg cgggtcacct tcggggtgca gtcactggtg
      361 cagaccacgt caaagggtga ccgaaacccg acatccgtcc gactgctgat tcagttacag
      421 cgtaacggta actgggtgac ggaaaaggat gtcaccatta acggcaagac cacctcgcag
      481 tttctggcgt cggtgattct ggataatctg cctccccggc cctttaacat ccggatggtc
      541 cgggagacag cggacagcac ctcggaccag ctgcagaata agacgctctg gtcgtcatac
      601 accgaaatca tcgatgtgaa acagtgctac ccgaacacgg cgattgtggg gctgcaggtg
      661 gatgcggagc agttcggcgg ccagcagatg acggtgaact accatatccg tggtcgcatc
      721 attcaggtac cgtcaaacta tgac
//
LOCUS       DAZFWL020000502          743 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFWL020000502 DAZFWL020000000
VERSION     DAZFWL020000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.508"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            33..710
                     /gene="tnpA"
                     /locus_tag="ACHKNP_RS28095"
                     /old_locus_tag="pgaptmp_005619"
     CDS             33..710
                     /gene="tnpA"
                     /locus_tag="ACHKNP_RS28095"
                     /old_locus_tag="pgaptmp_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HGU4707531.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
BASE COUNT          177 a          207 c          185 g          174 t
ORIGIN      
        1 tctggtctgt ttcctccggc tctacaaaaa taatgtccat catttttaat ggacactatc
       61 gtatgaaaca ccggacctgg atcactgaag ctttacgtct tcactttgaa gaacatttac
      121 cccgggttgt ggccgggcgt cgcctgggtg taccaaaatc aacagtttgt agtatgttcg
      181 tgcgctttcg gagagctggc ctttcgtggc ctttgcccgc aggcatgtcg gagcaggaac
      241 ttgatgcctg cctttacgga caattttcca cggtaccagt cgtacgtcct gaaagcaccg
      301 ttatatccga agcccccgtg gtaaaaaaac gtccccggcg gcccaacttc ccttatgagt
      361 ttaaaatcgc cttagtggag cagtcactgc agcccggagc ctgtgtggcg cagatcgccc
      421 gggaaaacgg aatcaacgat aacctgctct tcaactggcg ccatcaatac cggaaaggtg
      481 gcctgctgcc ttccggaaaa aatatgccgg cactgcttcc cgtgacgtta acgccggagc
      541 cggataataa aatcccggcc cccgcacagg aaccagagca gataaataca ccgtccgaca
      601 gtctgtgttg tgagctggtt ctgccggccg gaactctcag gcttaaaggt aaactgacgc
      661 cggcgttatt acagacactt atccgcgaaa taaaagggag cagccactga tgatatctct
      721 ccctgcaggt tcgcgtatct ggc
//
LOCUS       DAZFWL020000503          742 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFWL020000503 DAZFWL020000000
VERSION     DAZFWL020000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.509"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            118..645
                     /gene="sodC"
                     /locus_tag="ACHKNP_RS28100"
                     /old_locus_tag="pgaptmp_005620"
     CDS             118..645
                     /gene="sodC"
                     /locus_tag="ACHKNP_RS28100"
                     /old_locus_tag="pgaptmp_005620"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU4707532.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
BASE COUNT          195 a          194 c          181 g          172 t
ORIGIN      
        1 ggtattatta atcgcagtga taggatattg cacagctatt gcgcgatatc atcagaacgc
       61 tgtttgttac cctgtaacca gcaagctcag tctgttaacg gaattaatga gggttttatg
      121 aaatgtaaaa tcattgctgc cattgccatg ctgacagcag catcatgcgg atacgcagca
      181 gaacaggaag tcccaatgaa ccttgtcagt gctgacggaa aagaagtcag cattggaaaa
      241 ataaccattc aggagacccc ctacggtctg ctgttcacac cagcccttca ctctctgtct
      301 gaaggcattc atggttttca tgtgcacgaa aaaggaaatt gcgccccggc actgaaagac
      361 ggaaaaccgg tcgcagcatt atcggctggc ggtcactttg acccgaaaaa caccggcaaa
      421 catcttggcc cctggtctcc ggatggacac ctgggcgacc tccctgcgct gttcgtgacg
      481 catgacggaa aagcgaacta cccggtcctg gccccgagac tgaactcatt aaaagagatt
      541 aaagggcgtt ctctcatgct tcatgctggc ggtgataacc atcatgacca tccggagccc
      601 ctgggcggtg gtggtgcgag aatggcctgc ggcatcattc aataatcagt caggtaaggg
      661 gcgggcccct tacctttatt cctcaggacg ataaatcctt tctccctgaa aagaacggca
      721 catcctccct ctctgagtta at
//
LOCUS       DAZFWL020000504          738 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFWL020000504 DAZFWL020000000
VERSION     DAZFWL020000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.510"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            340..555
                     /gene="essD"
                     /locus_tag="ACHKNP_RS28105"
                     /old_locus_tag="pgaptmp_005621"
     CDS             340..555
                     /gene="essD"
                     /locus_tag="ACHKNP_RS28105"
                     /old_locus_tag="pgaptmp_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HGU4707533.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            555..>738
                     /gene="rrrD"
                     /locus_tag="ACHKNP_RS28110"
                     /old_locus_tag="pgaptmp_005622"
     CDS             555..>738
                     /gene="rrrD"
                     /locus_tag="ACHKNP_RS28110"
                     /old_locus_tag="pgaptmp_005622"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGU4707534.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIM"
BASE COUNT          186 a          142 c          178 g          232 t
ORIGIN      
        1 atttaaatcg gctaaaaata acgctggaaa ttatttgttg gttatttgtt gagatttgtt
       61 tatgtatttg tagtggtgtt ttcaatactc ggtagcattc tcgcaaatat catttagtgg
      121 tttacgtacg taaaaaattg gttatgctgt taagagtggt tacttcgtca cacagcttaa
      181 acccgccgtc gagcgggttt ttccattttt tgagtctcga tattagctga taacccaata
      241 cctgagttat tcactgactc cgagtctgtt acgtttctgc ttttttgcga tacgttgtat
      301 tccctcaatt tacacccgct ttgtctgcga ggtggggtta tgaaatccat ggataagtta
      361 acaacgggtg tcgcctatgg cacctcagca ggtagtgccg gttactggtt tttacagctg
      421 ctcgataaag tcacgccctc acagtgggca gcaataggtg tgctgggtag cctggtattt
      481 ggcctgctga cgtacctgac aaacctttat ttcaagatta aagaagataa gcgcaaggct
      541 gcgagaggtg aataatgcct ccatcattac gaaaagccgt tgctgctgct attggtggcg
      601 gagcaattgc tatagcatca gtgttaatca ctggcccaag tggtaacgat ggtctggaag
      661 gtgtcagcta cataccatac aaagatattg ttggtgtatg gactgtatgt cacgggcata
      721 caggaaaaga catcatgc
//
LOCUS       DAZFWL020000505          736 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFWL020000505 DAZFWL020000000
VERSION     DAZFWL020000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.511"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..245)
                     /locus_tag="ACHKNP_RS28115"
                     /old_locus_tag="pgaptmp_005623"
     CDS             complement(<1..245)
                     /locus_tag="ACHKNP_RS28115"
                     /old_locus_tag="pgaptmp_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4707535.1"
                     /translation="MQHIHGESLNESVKSEQSPRVVLWEIDLKAQGGERYFFCNELNE
                     KGEPVTWQGRKYQAYPIDGSGFEMNGKGSSARPSLTVS"
     gene            complement(245..586)
                     /locus_tag="ACHKNP_RS28120"
                     /old_locus_tag="pgaptmp_005624"
     CDS             complement(245..586)
                     /locus_tag="ACHKNP_RS28120"
                     /old_locus_tag="pgaptmp_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707536.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNVRYS
                     DFSCTIEQVVN"
BASE COUNT          156 a          262 c          158 g          160 t
ORIGIN      
        1 gacaccgtca gcgacgggcg ggcactgctg cccttcccgt tcatctcaaa gccactgccg
       61 tcaatcgggt atgcctggta cttccgcccc tgccaggtca ccggctcccc tttttcattc
      121 agctcattac agaaaaaata ccgctcacca ccctgggcct tcaggtcaat ttcccagagc
      181 accacccgcg gtgactgctc tgatttaacc gactcgttca ggctttcccc gtgaatatgc
      241 tgcatcagtt caccacctgc tcaatcgtac agctgaaatc actgtaccgg acgttatccg
      301 tgacgctcca ctcccggcac accaccctca ccgtccggtt atgtttcggc ggtcgccaca
      361 aaaaggcacg gtaaccacca tgccaggata aaaattcatc cagccagcgc cgggttgact
      421 catccgtcac ccggaacacc gcctgaaacg tcttcagttg aggattcagt cctgtggggc
      481 gacgctgttc ataaccgtca ccaaaccgta ccctcgccac cgacggtttc tcactcacct
      541 gcatcccttc acgcgggacc agatgcagcg tttttatctc agccactcag cattcctccg
      601 tcacgtcgca tggacaacat caccgcctgc acccgctggt caatcagctg cacaagactg
      661 cctgccgcct ccggccctat ctggccatta gtcccgtcat tctgaatggc gatatggtag
      721 accggggaat acacca
//
LOCUS       DAZFWL020000506          734 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFWL020000506 DAZFWL020000000
VERSION     DAZFWL020000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.512"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            5..734
                     /locus_tag="ACHKNP_RS28125"
                     /old_locus_tag="pgaptmp_005625"
                     /pseudo
     CDS             5..734
                     /locus_tag="ACHKNP_RS28125"
                     /old_locus_tag="pgaptmp_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          193 a          203 c          198 g          140 t
ORIGIN      
        1 tcacatgaca aaaacagtat caaccagtaa aaaaccccgt aaacagcatt cgcctgaatt
       61 tcgcagtgaa gccctgaagc ttgctgaacg catcggtgtt actgccgcag cccgtgaact
      121 cagcctgtat gaatcacagc tctacaactg gcgcagtaaa cagcaaaatc agcagacgtc
      181 ttctgaacgt gaactggaga tgtctaccga gattgcacgt ctcaaacgcc agctggcaga
      241 acgggatgaa gagctggcta tcctccaaaa ggccgcgaca tacttcgcga agcgcctgaa
      301 atgaagtatg tctttattga aaaacatcag gctgagttca gcatcaaagc aatgtgccgc
      361 gtgctccggg tggcccgcag cggctggtat acgtggtgtc agcggcggac aaggataagc
      421 acgcgtcagc agttccgcca acactgcgac agcgttgtcc tcgcggcttt tacccggtca
      481 aaacagcgtt acggtgcccc acgcctgacg gatgaactgc gtgctcaggg ttaccccttt
      541 aacgtaaaaa ccgtggcggc aagcctgcgc cgtcagggac tgagggcaaa ggcctcccgg
      601 aagttcagcc cggtcagcta ccgcgcacac ggcctgcctg tgtcagaaaa tctgttggag
      661 caggattttt acgccagtgg cccgaaccag aagtgggcag gagacatcac gtacttacgt
      721 acagatgaag gctg
//
LOCUS       DAZFWL020000507          731 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFWL020000507 DAZFWL020000000
VERSION     DAZFWL020000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.513"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..562)
                     /locus_tag="ACHKNP_RS28130"
                     /old_locus_tag="pgaptmp_005626"
                     /pseudo
     CDS             complement(1..562)
                     /locus_tag="ACHKNP_RS28130"
                     /old_locus_tag="pgaptmp_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          214 a          152 c          128 g          237 t
ORIGIN      
        1 cctgtttttg tatcttttct gcaatatctt tctggcagcc catcgcatca gttgtgatga
       61 tttttccttt aatatccatc atgttaagaa gttcaggaat ggctgtaatc tcattggatt
      121 tctcatccgt cttgatctgt ccgaggacca gactatgcat tgttgagaac gcactaatga
      181 catgaatcgc tcccctgcga cgactcttgt cataagagtg ccggagcgtt tttccatcaa
      241 ttgcaatgac gtctttatca tctgaagaat ggcagtcacg catccagtta ataaagcact
      301 cgtgaaattt tgcaggactg atacaggata caactctggc aatgatatcg tgaacaggaa
      361 taccattttc aaaatcacca tatcacttca aaacatcggg atgtgtttcc ccaaaatcct
      421 ctatatcttc ccaaccttct gcaccagaaa taacggcaca aatagtcaac agtagaatat
      481 ccgataattt atgttccact ttccaggttt gtctgtaatc ggggataata gaaatatgtt
      541 ccatcaattt tttaagttcc attttgttct ccttaattag gtaaggagta tttgatcatg
      601 tataagcaat aaaaaacagc ttcaggtaat aaggaatatc tcaattttta aacataaaat
      661 gcgaattatt tagtgcaaaa agtgaacttt tgatgatctt tccctggccg ttgactcgat
      721 tgccgtagct c
//
LOCUS       DAZFWL020000508          725 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFWL020000508 DAZFWL020000000
VERSION     DAZFWL020000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.514"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>725
                     /locus_tag="ACHKNP_RS28135"
                     /old_locus_tag="pgaptmp_005627"
     CDS             <1..>725
                     /locus_tag="ACHKNP_RS28135"
                     /old_locus_tag="pgaptmp_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HGU4707537.1"
                     /translation="TLPETGAATVNLINGSGKPVSVAITAHPAPDRIQVSTLPDGVET
                     YGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGASFEPQSGTLN
                     SVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGQDSRLVTTAIT
                     ADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPAVPATIELTPGYFQITA
                     VPRLAVYDPTVQFEFWFSETKIA"
BASE COUNT          149 a          215 c          232 g          129 t
ORIGIN      
        1 accctgccgg agacaggtgc cgccacggtg aacctgatta acggcagcgg taagccggtg
       61 agcgtggcca tcactgcaca ccccgcgccg gaccggatac aggtcagcac cctgcctgat
      121 ggtgtggaga catacggtgt atgggggctc tccctgccgt cactgcgtcg tcgcctgttc
      181 cgctgtgttt ccatccggga aaacacggac ggtacctttg ccatcacggc agtgcagcat
      241 gtgccggaga aagaagccat cgtggataac ggggccagct ttgagccgca gtcaggcacc
      301 ctgaacagcg tcatccctcc ggcagtgcag cacctgacgg tggaggtgag cgcagctgac
      361 ggccagtatc tggcgcaggc gaaatgggac acgccgcggg tggtgaaggg cgtgcgcttc
      421 agtctgcgcc tgaccagtgg aagcggtcag gacagccgtc tggtgaccac cgccatcacc
      481 gcggatacag agcaccgttt cagcggtctg ccgccggggg aatacaccct gacggtcagg
      541 gcgattaaca gttatggcca gcagggggaa ccggccacca ccacgttcag gattaatgca
      601 cctgcggtac ccgccacgat tgagctgaca ccgggctatt ttcagataac agcggtcccg
      661 cgtcttgcgg tgtatgaccc gacggtacag tttgagttct ggttttcgga gacaaaaatc
      721 gcaga
//
LOCUS       DAZFWL020000509          724 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFWL020000509 DAZFWL020000000
VERSION     DAZFWL020000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.515"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            102..653
                     /locus_tag="ACHKNP_RS28140"
                     /old_locus_tag="pgaptmp_005628"
     CDS             102..653
                     /locus_tag="ACHKNP_RS28140"
                     /old_locus_tag="pgaptmp_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGU4707538.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
BASE COUNT          235 a          146 c          148 g          195 t
ORIGIN      
        1 taaaacccga aaagtaaaat ataatatttg aatgattagc aacccttaaa taacagttac
       61 taatcattca ttttctactt atatttatat aaaggaatat tatgtctgct tttaaaaagt
      121 cacttcttgt tgctggcgta gcaatgattc tttccaataa cgtatttgct gacgaaggtc
      181 acggcattgt taaatttaaa ggtgaagtta tctctgctcc atgttcaatt aaaccaggtg
      241 acgaagactt aactgtaaat ctgggtgaag tagcagatac cgttctgaaa agcgaccaga
      301 aatctttggc tgagcctttc actattcacc tgcaagactg catgttgagc cagggtggaa
      361 ccacttattc aaaagcgaag gttactttca ccacagcgaa tactatgacg ggtcagactg
      421 acctgctgaa aaacaccaaa gaaactgaaa tcggtggtgc cacaggcgtt ggcgtacgta
      481 ttctggatag ccagagcggc gaagtgactt taggtactcc ggtagtaatt acattcaaca
      541 atactaacag ctatcaggaa ctgaacttca aagctcgtat ggaatcccca agcaaagatg
      601 ctaccccggg caacgtttac gctcaggctg actataagat tgcttacgag taatcacatt
      661 aggccgggtt cgcccggcca catcattaac ggattaatga taagtggaac agatgtataa
      721 aaaa
//
LOCUS       DAZFWL020000510          709 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFWL020000510 DAZFWL020000000
VERSION     DAZFWL020000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.516"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..615
                     /locus_tag="ACHKNP_RS28145"
                     /old_locus_tag="pgaptmp_005629"
     CDS             <1..615
                     /locus_tag="ACHKNP_RS28145"
                     /old_locus_tag="pgaptmp_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HGU4707539.1"
                     /translation="CTIKEVQFTAGQKQDIDVTTLCSTEQENINGLGASSEISMSGNF
                     YLNQAQNALRDAYDNDALYAFKVLFPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRM
                     KGKPVSFVVPLAFVKNLDKTLTVNTGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTT
                     DTFSKPGAQSADAGKYTCVVTDSAEKAQSVTSVECTVTVSAAAG"
     gene            582..>709
                     /locus_tag="ACHKNP_RS28150"
                     /old_locus_tag="pgaptmp_005630"
     CDS             582..>709
                     /locus_tag="ACHKNP_RS28150"
                     /old_locus_tag="pgaptmp_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU4707540.1"
                     /translation="MHRDSERSRRIRGWVIMKKDLKTLALARLSGFRHKTVKVPEWG"
BASE COUNT          174 a          165 c          219 g          151 t
ORIGIN      
        1 tgcaccatca aggaagtcca gttcaccgcc ggtcagaaac aggatattga tgtgaccacg
       61 ctctgctcca cagagcagga gaacatcaac ggtctggggg cgtcgtctga gatttccatg
      121 tcgggtaatt tttatctgaa tcaggcccag aacgccctgc gtgatgccta tgacaatgac
      181 gcgttgtatg cgtttaaggt gctgtttccg tccggtaagg gctttaagtt cctggcggaa
      241 gtgcgtcagc acacctggtc atccggtacc aacggcgtgg tggcagcaac gttttcactg
      301 cgtatgaaag gcaaaccggt gtcctttgtg gtaccgctgg cgtttgtgaa aaatctggat
      361 aaaacactta ccgtgaatac aggtgcgctg ctgacaatgt cagtcagtgc caacggggga
      421 acgccgccgt ataaatacgc ctggaagaag gatggtcagc cggttgacgg gcagacgaca
      481 gacaccttca gtaagccagg tgcgcagtcc gctgatgcgg ggaaatatac ctgcgtggtg
      541 accgattcgg cagagaaagc acagagtgtg acgtctgttg aatgcaccgt gacagtgagc
      601 gcagccgccg gataagggga tgggtcatca tgaaaaagga tctgaaaacg ctggcgctgg
      661 ccagactgtc agggtttcgt cataaaacgg tgaaggtgcc ggaatgggg
//
LOCUS       DAZFWL020000511          707 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFWL020000511 DAZFWL020000000
VERSION     DAZFWL020000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.517"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>707
                     /locus_tag="ACHKNP_RS28155"
                     /old_locus_tag="pgaptmp_005631"
     CDS             <1..>707
                     /locus_tag="ACHKNP_RS28155"
                     /old_locus_tag="pgaptmp_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4707541.1"
                     /translation="PKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAG
                     QCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENGVNV
                     PTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSAWARRGIISDRLATAILRHA
                     GRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASESEGSLKVQGWSASGGRAEV
                     VSDAEGTGGKAVKLTKEA"
BASE COUNT          174 a          166 c          231 g          136 t
ORIGIN      
        1 ccgaaaaatg tgctgtttgc cgtggtgtgg atgcaggggg aatttgattt tggcggtacg
       61 ccggcaaatc acgcagcaca gtttggtgcg ctggttgata aattccgtgc agacctggcg
      121 gatatggcag gtcagtgcgt cggtggctct gctggcggtg ttccctggat atgtggagat
      181 acgacgtatt tctggaagca gaagaacgaa tccacgtacc agacggtgta cggcagctat
      241 aaaaacaaaa cggaaaagaa tatccatttc gtaccgttca tgaccgatga gaacggggtg
      301 aatgtgccga cgaacaaacc ggaagaagac ccggacattc cgggtatcgg atattacggt
      361 tcgaaatggc gtgacagctc agccacctgg acgtcacagg acagggcgag ccatttcagc
      421 gcctgggcac gccgtgggat tatttccgac cgtctggcaa cggcgatttt gcgccatgcg
      481 ggaagagtgg cgctaaacgc gggggcatca tcgacagtat cagaggtgcg cccttcatcg
      541 ccttccggtg cagaagccac aggcgtcaca acactgctct cttaccttgc cagcgagtca
      601 gagggaagcc tgaaagtaca gggatggtca gccagtggcg gcagggcaga agtggtcagc
      661 gatgcggagg gaaccggagg taaggcagtg aagctgacca aggaggc
//
LOCUS       DAZFWL020000512          707 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFWL020000512 DAZFWL020000000
VERSION     DAZFWL020000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.518"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            412..>707
                     /locus_tag="ACHKNP_RS28160"
                     /old_locus_tag="pgaptmp_005632"
     CDS             412..>707
                     /locus_tag="ACHKNP_RS28160"
                     /old_locus_tag="pgaptmp_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4707542.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPVSNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEEDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTG"
BASE COUNT          193 a          125 c          187 g          202 t
ORIGIN      
        1 tgttcttcgt tgacatgagg ttgccctgta ttcagtgtcg ctgatttgta ttgtctgaag
       61 ttgtttttac gttaagttga tgcagatcaa ttaatacgat acctgcgtca taattgatta
      121 tttgacgtgg tttgatggcg tagatgcacg ttgtgacatg tagatgataa ttattatcat
      181 tttcgtgggt cctttccggc gatccgacag gttacggggc ggcgacctcg cgggttttcg
      241 ctatttatga aaattttccg ggatccatgt ccggtttctc ttcaagttaa ctatatgaaa
      301 aatataaaaa caggtcttct gtgaaccgga catgaacaaa aaacagacat gtaaaccgga
      361 catgaccggt tttgttgtga ttgtgaggtg agagtttttg cgaggtgagg agtggctacg
      421 cagactgaag ttgccaggca tttaagtctg accgatcgcc agcttcgcag attgcagaaa
      481 ttaccgggtg ccccggtctc gaataagcga gggcaactgg atctggatgc ctggcgcgat
      541 ttttacatat cgtatctgag aagaagtaaa aacgatgtgc ctgatggcga tagcgaagaa
      601 gactatgaag agaaattgct tattgccaga tgggaactga cagcagaaca ggctgttaca
      661 cagcagttaa aaaatgaggt gtcaaaagga aaactgattg acaccgg
//
LOCUS       DAZFWL020000513          706 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFWL020000513 DAZFWL020000000
VERSION     DAZFWL020000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.519"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..698
                     /locus_tag="ACHKNP_RS28165"
                     /old_locus_tag="pgaptmp_005633"
     CDS             <1..698
                     /locus_tag="ACHKNP_RS28165"
                     /old_locus_tag="pgaptmp_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HGU4707543.1"
                     /translation="PAVLFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRI
                     TEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKT
                     KPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENAL
                     RMVSLGRKNYLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRV
                     GELLPWRVALPTE"
BASE COUNT          167 a          196 c          220 g          123 t
ORIGIN      
        1 cgccggcggt gttgttcgct tacagcccgg acagaaaagg catccatccg cagacccatc
       61 ttgcggggtt cagtggtgta ctgcaggcgg atgcatacgc cgggttcaac gagctgtacc
      121 gggatggccg gataacggaa gccgcctgtt gggctcacgc ccgccgtaaa atccacgatg
      181 tgcacgttcg caccccgtca gccctgacgg aggaagcgct gaaacggatc ggcgaactgt
      241 acgccatcga ggcagagata aggggaatga cggcggagca gcgccttgcc gaacgtcagt
      301 tgaaaacgaa accgctgctg aaatccctgg aaagctggct gcgtgaaaag atgaaaaccc
      361 tgtcgcgaca ctcagaactg gcgaaagcgt tcgcatacgc cctgaaccag tggccggcgc
      421 tgacgtacta tgcagatgat ggctgggctg aggcggacaa taacatcgct gaaaatgcgt
      481 tgcggatggt cagtctgggc cgcaaaaact acctgttctt cggttcggat catggaggag
      541 agcggggagc gctgctgtac agcctgatcg ggacgtgcaa actgaacgga gtggagccag
      601 aaagctacct ccgctatgtc cttgacgtca tagccgactg gccgataaac cgggtcggcg
      661 aactgctccc ctggcgcgta gcactgccga ctgaataaca catccc
//
LOCUS       DAZFWL020000514          706 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFWL020000514 DAZFWL020000000
VERSION     DAZFWL020000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.520"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            80..505
                     /locus_tag="ACHKNP_RS28170"
                     /old_locus_tag="pgaptmp_005634"
     CDS             80..505
                     /locus_tag="ACHKNP_RS28170"
                     /old_locus_tag="pgaptmp_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4707544.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            502..576
                     /locus_tag="ACHKNP_RS28175"
                     /old_locus_tag="pgaptmp_005635"
                     /pseudo
     CDS             502..576
                     /locus_tag="ACHKNP_RS28175"
                     /old_locus_tag="pgaptmp_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          203 a          167 c          167 g          169 t
ORIGIN      
        1 gtaaactgac cgccgtatgt agccatcaga cgaaaattgg taacttagac gcccatctga
       61 tatagacgga catctaagta tggaattaca ggactggcga aaagaacctc gtaaaaacta
      121 ttcgaatgaa ttcaaacttc gtatggtgga actggcatca caaccaggag cctctgttgc
      181 ccgtattgcc cgggaacacg atatcaatga taacctcctg ttcaaatggc tcaggctctg
      241 gcagaacgaa gggcgtatat cacggcgtct tcaggtaacg acctcttctg acactggcgt
      301 tgaattatta cctgtagaaa taacgccgga tgagcagaaa gaaccggtgg cggccattgc
      361 gccgtcttta tccacttcca ctcagaccag tgtcagtgcc ggctcctgca aggtggagtt
      421 ccgtcacggt aacatgacgc tggaaaatcc atcgccagag ctgctcacgc tgttgatccg
      481 tgaactgacc gggaggggac gatgatctca ctcccgtcag gtacccgcat ctggctcgtt
      541 gccgacgtta ccgatatgcg taaatccttc aacggatacg acatatgctg aaggaggagc
      601 agtaatggat taagtgataa caaataccca gaaatagagg ttcaaatcca gcttagttag
      661 tacataaata ctattttgat gaaaattatt atatgtttcc tcagat
//
LOCUS       DAZFWL020000515          699 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFWL020000515 DAZFWL020000000
VERSION     DAZFWL020000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.521"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(312..>699)
                     /locus_tag="ACHKNP_RS28180"
                     /old_locus_tag="pgaptmp_005636"
     CDS             complement(312..>699)
                     /locus_tag="ACHKNP_RS28180"
                     /old_locus_tag="pgaptmp_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4707545.1"
                     /translation="PLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAVRR
                     AEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFALDM
                     AATLVVPFTGDGRDAAEILRTLPPLK"
BASE COUNT          144 a          199 c          182 g          174 t
ORIGIN      
        1 cgtcatagca gactggccgg tcaaccgggt cagcgaactg cttccgtggc gcatagcact
       61 gccagctgaa taacacatcc ccgtcaatac ggccctcgct gtacgcttac gtttatattc
      121 agtttcctgc agtgctgcct gtggctgagc tgccatctgc ctgtccctta cgtgagtcac
      181 cccgtaacct gatgctgagg cattgctccc ttcataaaac atggtttact cactacagct
      241 tatctacctg ctccagctta tgttatgtct gttctgctga ccactgctta tataaggaag
      301 agcgtatgct gtcacttcag gggaggcagt gtacgcagga tctctgcagc atcgcgcccg
      361 tcacctgtga aaggcactac cagcgtggca gccatatcca gcgcaaaaac tctggtataa
      421 acctccatcg aacgtggatc cctgtgacca gccagtgcct ggatgacttt ccggggctgg
      481 cggtgataga gcatgtgcat gatatagctg tgccggaagg tgtgtggtgt gaccggaatc
      541 gaaaagtgta ccccgtcagc ttcggcccgt ctgacagcct gcttcagcca gttgcgcatg
      601 gtttcgtcag tcacggccca taatggttca cgacgacggg gccgggtggt gatcatccag
      661 ctttccatct gcctgacata gcttatatct gtcagcgga
//
LOCUS       DAZFWL020000516          698 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFWL020000516 DAZFWL020000000
VERSION     DAZFWL020000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.522"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            32..595
                     /locus_tag="ACHKNP_RS28185"
                     /old_locus_tag="pgaptmp_005637"
     CDS             32..595
                     /locus_tag="ACHKNP_RS28185"
                     /old_locus_tag="pgaptmp_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4707546.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
BASE COUNT          208 a          131 c          171 g          188 t
ORIGIN      
        1 cctgtaactt ttataaagta aggactataa aatgtataat gctaatccca attacgaaat
       61 ggacttcatg attctgaaag acgtgaatga acacatggaa ggtctgtttc aacgtttttc
      121 taaactgtta cctttcagaa ttgattttgc ctacaggaaa gacacgccca gttttggtca
      181 ttcctgtagg cacagtatgt gtattgaaat gcaccgctta cttagtgaaa cccaaacgat
      241 gctggcggga tattactggg taatggaata tacgtcaaac aagggacttc atattcattt
      301 tatcggctac ctggatgggc aacgtcacaa caaatcttat cggatatcca ggcaactggg
      361 agatatctgg aggcggatca cggaagggga gggttatttt cacctgtgca gggctaaaga
      421 caaatacccg gtgcgtatcg atcacgtaat tcactatagt gataaatctg cggtagacga
      481 cttacgttac gcccttagct atctggccaa acaggatcag aaagaacacg gtattattct
      541 gaggcgtagc aggcttcccg aaaaaagtaa ccggggccga ccacgacata actgaattac
      601 agctgtatta ggtattagaa ttaagtttct gtaagttgtt gggaggtgct tgagttaata
      661 ggcagaagag ggcggggcgc tttgtcatca gtgtaggg
//
LOCUS       DAZFWL020000517          693 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFWL020000517 DAZFWL020000000
VERSION     DAZFWL020000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.523"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>693
                     /locus_tag="ACHKNP_RS28190"
                     /old_locus_tag="pgaptmp_005638"
     CDS             <1..>693
                     /locus_tag="ACHKNP_RS28190"
                     /old_locus_tag="pgaptmp_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4707547.1"
                     /translation="GQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSALT
                     KTERELALSRLKGEEKERARLGYAADDLGFVGDSYQEARQRYISNALEAWRNNEANKP
                     KSRGRKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKTLTEMQKQE
                     LLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARERMRELQQI
                     RDSFRQKDADLQS"
BASE COUNT          193 a          160 c          228 g          112 t
ORIGIN      
        1 ggtcagcata cggaattcaa ccgtctgctg gggctgggga atgaactgct tcagcagcgt
       61 caggggctgg tgaatgtacc gttacggctg ccacaggcca ccctggatga taaacagcag
      121 agtgccctga caaaaacaga gcgtgagctg gccctgtccc gactgaaggg ggaggaaaaa
      181 gagcgtgccc ggctggggta tgcggcggat gaccttggtt ttgtggggga ttcgtatcag
      241 gaggcgagac agcgttatat cagtaatgct ctggaagcct ggcgtaataa cgaggcgaac
      301 aaacctaaat cccggggtag aaaatcagag acggaaaaag cggaagacag tttttcccgg
      361 ctactgaagc agcagaaaga gcaactggca ctggcgggtc agaatacaga gctggcgaag
      421 ctgaagtacc agactgcgca gggcgaactg aaaaccctga cggagatgca gaagcaggaa
      481 ctgctgcgta acgcggccct gattgaccag caaaaaatcc gggaacagtt gcgatcccgg
      541 gaagagaccc tgaagaatga taatgtggct gcgcgtgcat caaatgaagc cgaactgctg
      601 ggatacgggc agggagaacg agccagggaa cgcatgcggg agttgcagca gatccgcgac
      661 agcttccgcc agaaggatgc ggaccttcag tct
//
LOCUS       DAZFWL020000518          688 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZFWL020000518 DAZFWL020000000
VERSION     DAZFWL020000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.524"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..605)
                     /gene="sctD"
                     /locus_tag="ACHKNP_RS28195"
                     /old_locus_tag="pgaptmp_005639"
     CDS             complement(<1..605)
                     /gene="sctD"
                     /locus_tag="ACHKNP_RS28195"
                     /old_locus_tag="pgaptmp_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     subunit SctD"
                     /protein_id="HGU4707548.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADQSLPVGKVIDIAGCCFIIGDIDHTLP
                     LSDVPERFSAKNRRKKRLILASVIGAAIALSGAIGSYVLLSPKAEPPAFTRADVYQQL
                     KENKLHAITLVWHGKNVALYGRCESTTDLTPFFNYLKEKNI"
BASE COUNT          213 a          135 c          145 g          195 t
ORIGIN      
        1 aaaatatttt tttctttcag gtaattaaaa aatggagtta aatcagtggt tgattcgcaa
       61 cgcccataaa gcgcgacatt tttcccatgc cataccagag tgattgcatg aagtttgttt
      121 tcttttagct gctggtatac atcagctctg gtaaacgctg ggggttcagc ttttggggaa
      181 agcaaaacgt aactgccgat ggcgccagac aacgcaatcg cagcaccaat cacgctagcc
      241 aggataagac gctttttacg tcgatttttg gcagagaacc tttctggcac atcagacaat
      301 ggcaaggtgt ggtcaatatc accaatgatg aagcaacatc ccgctatatc gatgaccttt
      361 ccgacaggta atgactgatc tgcttcatag ggggtgggct gtccatcaat ccaaaattca
      421 accggagata aaaggaaaac accttcctca cgtatatcaa gtaaaaactg attgaggcct
      481 tgttcaaggg gaaaataaac aatatcgtta tcttctgtgc ctatcgttag gtttcccatc
      541 ggaagttgta attcccgatt acgcatcgca ccattgagaa gttttatttt atatgaggat
      601 aacattttta aattttattc atcctggtgg ttgaacaatg agaaaaaata tggtgaactt
      661 acatcaacta agaatagagt agaaagga
//
LOCUS       DAZFWL020000519          688 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFWL020000519 DAZFWL020000000
VERSION     DAZFWL020000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.525"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..620)
                     /locus_tag="ACHKNP_RS28200"
                     /old_locus_tag="pgaptmp_005640"
     CDS             complement(<1..620)
                     /locus_tag="ACHKNP_RS28200"
                     /old_locus_tag="pgaptmp_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4707549.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVP"
BASE COUNT          167 a          174 c          168 g          179 t
ORIGIN      
        1 ggcaccacgc cgatggtgtt ttcgtcacag gcttcaatca tgcgtttcgg gtccataaac
       61 aactgaccgg ggcgcatagg gatctcacgc agctccacat cccagtagcg ggcgaattta
      121 tgccagcaga tttgtaccgg accgcacacc aggtttggtt tatccgttgg tttgcctgca
      181 gcttccatac gcttgcgcca acgccatttc atcgccatcc cgccgagcat acaggcctcg
      241 gaagaaccaa tggtgttggt gccaacggcc tgaccatttt tcggcgcagg cgcatgccac
      301 agatcggcaa ccatatttac gcaacgcagg tcgatggctg cggattgcgg atattcttct
      361 ttgtcgatcc agtttttatt gatcgacaaa tccatcaatt tatggacgtt ttcgtcgtcc
      421 caggtctggc agaaagtggc caggttctga cgagcgttgc catcaagata taattcatca
      481 ttgataatct ggaatgcgac atcatcgcgc atttcgtgca gcggaaatcg ttttgactcc
      541 gcgatagtag aaatggcctt tgcgccaaaa cgtgaatcga gtagttctga gcggaaatcc
      601 gttaacagct tctggtccat ttcgaactcc ttaaatttat ttgaaggcaa taaaaaagta
      661 ggatttatcc gcaatggaag caaggcat
//
LOCUS       DAZFWL020000520          683 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFWL020000520 DAZFWL020000000
VERSION     DAZFWL020000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.526"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>683
                     /locus_tag="ACHKNP_RS28205"
                     /old_locus_tag="pgaptmp_005641"
     CDS             <1..>683
                     /locus_tag="ACHKNP_RS28205"
                     /old_locus_tag="pgaptmp_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4707550.1"
                     /translation="QRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTGLKI
                     SGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLKAMA
                     DDGQLTADKVVPALISQLGVLRDEYAAMPETVSGSITKVENAFMAWVGGVNEASGVTK
                     TLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVALAE
                     AQFRGTQIAT"
BASE COUNT          139 a          155 c          238 g          151 t
ORIGIN      
        1 cagcggacag gtaccgcttt ttcggataat gcggccctgt ttgcccgttc ggctgcctcg
       61 atgcgtgaat atggttacag tgctgatgat gtgctgaagg tgacggaggc catttccacg
      121 gggctgaaaa tctccggtgc cagtacggct gaggcgggtt cggtgatcac ccagttcagc
      181 caggcgctgg cacagggtgt gttgcgcggt gaggaattta attcggtcaa tgaaagtggt
      241 gaccggatca ttcgtgcact ggctgcaggc atgggcgtgg cccgtaaaga tctgaaggcg
      301 atggcggacg atggtcaact gacggcggat aaagtcgttc ctgcgttaat cagccagctg
      361 ggggtattgc gtgatgaata tgccgccatg ccagaaacgg tttccggtag tatcacgaag
      421 gtggaaaacg cctttatggc ctgggtgggc ggagtgaatg aggccagcgg ggtgacaaaa
      481 acgctctccg gcgtgctgaa cggtgttgcc ggacagattg ataatgtggc aacagctgtg
      541 ggtgcgctgg ttgccgtcgg ggttgcccgg tactttggca atatggcctc cggagcgatg
      601 tctgccacgg caggacttgt gacggctgca cgtaatgaag ttgcactggc ggaagcacag
      661 ttcaggggaa cgcagattgc cac
//
LOCUS       DAZFWL020000521          679 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFWL020000521 DAZFWL020000000
VERSION     DAZFWL020000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.527"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>679
                     /locus_tag="ACHKNP_RS28210"
                     /old_locus_tag="pgaptmp_005642"
     CDS             <1..>679
                     /locus_tag="ACHKNP_RS28210"
                     /old_locus_tag="pgaptmp_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HGU4707551.1"
                     /translation="LSGIIHNIENSIISGHESSDIIRSHQHEWGDLSTVEQFKKFEFY
                     VKSELSFSKSIFDDIKTKYITDPETKRNALYHQLDSDIKERIAFLDISHYAYPGSLLE
                     KLQLSGYVFSDINIIAEYLLSSYGISGHYSHGVVYPAPSDKLFELLRRHTNSNSDWIE
                     KIIPYVYDILSGNVSSFLHPPLSEEQKKILSDIKLEISESVSEQYFMKLTEQKSSVIG
                     IKYSVDFD"
BASE COUNT          249 a          112 c          105 g          213 t
ORIGIN      
        1 ctatcaggca ttatacacaa catcgaaaat tctattatat caggtcatga atcatctgat
       61 ataatacgaa gccatcaaca tgagtgggga gatttatcaa cggtagagca atttaaaaaa
      121 tttgaatttt atgttaaaag tgagttatca ttttccaagt caatttttga tgatataaaa
      181 accaaatata taacagatcc cgaaacaaaa cgaaacgcac tttaccatca attagatagt
      241 gatatcaaag agcgaatagc ctttctcgac atatcccact acgcatatcc aggtagttta
      301 ttagaaaaat tacagttaag cggctacgtt ttttctgaca tcaacataat tgcagaatac
      361 ttattgtcta gttacggaat ttctgggcat tattctcacg gcgtggtata cccagctcca
      421 tctgataaat tatttgagtt attaagaaga catacaaatt ccaattctga ttggatagaa
      481 aaaataatcc catatgttta tgatatctta tcaggtaatg tgtccagctt cttgcaccct
      541 cccttatcag aagaacagaa aaaaatattg agtgatataa aattagaaat tagtgaatct
      601 gtatcagaac agtatttcat gaagttgaca gaacaaaaat catcagttat tggcattaaa
      661 tactctgttg attttgata
//
LOCUS       DAZFWL020000522          678 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZFWL020000522 DAZFWL020000000
VERSION     DAZFWL020000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.528"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..311
                     /locus_tag="ACHKNP_RS28215"
                     /old_locus_tag="pgaptmp_005643"
     CDS             <1..311
                     /locus_tag="ACHKNP_RS28215"
                     /old_locus_tag="pgaptmp_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707552.1"
                     /translation="IRAGVQYYDRQTKKKEWTNYSAVVFAKPGAQADYYRSVLVEGGI
                     VEITGENIRVDVYQGQNGQSITLELLNAKIGFAASGNGPQQQSSNQQNTPVYDDSIPF
                     "
     gene            335..628
                     /locus_tag="ACHKNP_RS28220"
                     /old_locus_tag="pgaptmp_005644"
     CDS             335..628
                     /locus_tag="ACHKNP_RS28220"
                     /old_locus_tag="pgaptmp_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HGU4707553.1"
                     /translation="MPAPLYGADDARRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
BASE COUNT          206 a          155 c          162 g          155 t
ORIGIN      
        1 acatccgtgc tggcgttcag tattacgatc gccagacaaa aaagaaagaa tggacaaact
       61 acagcgccgt tgtatttgcc aagccgggag cgcaagcgga ttactaccgt agtgttcttg
      121 ttgaaggtgg cattgtggaa attaccggag aaaacatcag ggttgatgtt tatcaggggc
      181 aaaatggtca atcaatcact cttgaattac tgaatgcaaa gattggattt gcagcttcag
      241 gaaatggccc gcagcagcaa agtagtaacc agcagaacac tcctgtatac gacgattcca
      301 tcccattctg atttagaaaa ataaggattt aattatgcca gcgcctctgt atggtgcgga
      361 tgacgcgcgc cgctgttccg gcaattccgt atcggaggtg ctggataaat tcagaaaaaa
      421 ctacgatcgg ataatgtcgc taccgcagga aacgaaagag gaaaaggaat ttcgccattg
      481 tatatggctt gcagagaaag aagaacgcga gcgaatttac cagacatcaa tccgaccatt
      541 ccgcaaagcc acatataccc acttccctga aattgacccg cgcctgcgta attaccgctc
      601 acgctatggc gctatcagta atgactgagg aatttaccat gagaggactt gcatacaatc
      661 ccggcattct tccggcag
//
LOCUS       DAZFWL020000523          678 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFWL020000523 DAZFWL020000000
VERSION     DAZFWL020000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.529"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..140)
                     /locus_tag="ACHKNP_RS28225"
                     /old_locus_tag="pgaptmp_005645"
     CDS             complement(<1..140)
                     /locus_tag="ACHKNP_RS28225"
                     /old_locus_tag="pgaptmp_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4707554.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DER"
     gene            complement(230..598)
                     /locus_tag="ACHKNP_RS28230"
                     /old_locus_tag="pgaptmp_005646"
     CDS             complement(230..598)
                     /locus_tag="ACHKNP_RS28230"
                     /old_locus_tag="pgaptmp_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4707555.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
BASE COUNT          155 a          157 c          214 g          152 t
ORIGIN      
        1 cgttcatcgg cgaacggtgt gtcattcagt gtaaggccgt aatgctggcc cagtagtcgg
       61 gagagcagag tctgccagat ggtgacggga gacgggcagc tcgatgcctc ccgtacgtgt
      121 gtatcgggta atgttttcat actgaaggtt ttcctgatat gcagagatga aaaatggaaa
      181 agtggcgtgg tgaaagcacc aggccggagc agaaggttat tccggtggtt tactttttcg
      241 tttcgggcgt cggataaaca gccagataaa cgtaaccacc gctaccgaga gtgtcagctt
      301 tgcaggtcag accttttgca tacagcgtga cggtatgctg atggcgggga ttcagttcac
      361 cgctggtgag catgagttcc agttgtttca tcagcagcgg aaaggcctgg tccaggtggt
      421 acgcatctgc atcgctgaac aggcctctga taccggcgcg gtcggcaagg taatgcaggc
      481 ggttaccctc ctgcaccaga cgggcgccga aacagggcgt cactgtgcag ggtagtcccc
      541 accagggatg gtcgtcattg tcatcgggat gcgttgtccc ggaaagtgtg tctgacacga
      601 taaaatcctc acagaaaatc ggtgaatgat agtttaacga tgacgggtca gccggcgcgc
      661 agaccggctt cggtaaag
//
LOCUS       DAZFWL020000524          677 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZFWL020000524 DAZFWL020000000
VERSION     DAZFWL020000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.530"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..115)
                     /locus_tag="ACHKNP_RS28235"
                     /old_locus_tag="pgaptmp_005647"
     CDS             complement(<1..115)
                     /locus_tag="ACHKNP_RS28235"
                     /old_locus_tag="pgaptmp_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707556.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVFYGH"
     gene            complement(99..>677)
                     /locus_tag="ACHKNP_RS28240"
                     /old_locus_tag="pgaptmp_005648"
     CDS             complement(99..>677)
                     /locus_tag="ACHKNP_RS28240"
                     /old_locus_tag="pgaptmp_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4707557.1"
                     /translation="NRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLD
                     AIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAMRHCYLM
                     CRMTKTFGSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACI
                     EKYNTGQLFGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          197 a          164 c          114 g          202 t
ORIGIN      
        1 tatgtccata aaatacgatg tcagaatttg aattgttatc aaacccaagg taaacaacaa
       61 ataaacaaag ggtaattaaa aaaatgacaa gtttgcctct aataatttga agcatctgaa
      121 ctcccttgct ttgcttttat tggattatct gcttttatac catctaaccc gaagagttgc
      181 ccagtgttat atttttcaat acatgcatcg ctacatttgg cagaaaaatc gccacaagca
      241 ataccgacag tgttattttt aagatccatg attctttctt tagctggctg accttgtcta
      301 tcccctgccg cctcatgatt tttacctatc acgtcagcta ttgttgatcc aaatgtctta
      361 gtcattcgac acatcaagta acaatgtctc attgcatcgg cggccccatt atgagctccg
      421 ccaacaatag catccatatc ataaccgaac tggtaagcac tgttagtaat gctgtttgca
      481 gcaatcaatc caggtacacc agatacggca taaattagtc cacctgatgt catattctca
      541 atcgcatcta accccattgg gtctatcact tgtatgggat tcaacggata ctgatacata
      601 ttccatccac ctctcaaccc aatcgggtcc ggggtgatat accgcccctg caacggatcg
      661 tagtaccggt gacggtt
//
LOCUS       DAZFWL020000525          672 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZFWL020000525 DAZFWL020000000
VERSION     DAZFWL020000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.531"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            385..>672
                     /gene="gadE"
                     /locus_tag="ACHKNP_RS28245"
                     /old_locus_tag="pgaptmp_005649"
     CDS             385..>672
                     /gene="gadE"
                     /locus_tag="ACHKNP_RS28245"
                     /old_locus_tag="pgaptmp_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HGU4707558.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIK"
BASE COUNT          240 a          102 c          125 g          205 t
ORIGIN      
        1 gttaaaagaa agctggcaat ccaattgcca gcttaagtcg aaacaaggag actcgatatt
       61 taaatcggat tacattttaa ctttagtaat attcttcaga gatcacaaac tggttattga
      121 taacttattc ttggccagta atccgcaaac gttaactttt tgtttgatat ttacaaattg
      181 ataacaacca ggaatcttac ttaggatcaa tatatggagt gcgtgatgga taaatctgaa
      241 gtattgatta gtgttaatag acgtattagt tcacgaaggg caaagttctt ataggcgttt
      301 actatattga acaacgattc ggacaaggat gtaaataatg aaaaggatga catgttcgaa
      361 acgataacgg ctaaggagca agttatgatt tttctcatga cgaaagattc ttttctttta
      421 cagggctttt ggcagttgaa agataatcac gaaatgataa aaatcaattc cctgtcagag
      481 atcaaaaaag taggcaataa acccttcaag gttatcattg atacctatca caatcatatc
      541 cttgatgaag aagcgattaa atttctggag aaattagatg ccgagagaat tattgttttg
      601 gcaccttatc acatcagtaa actaaaagct aaagcgccta tttattttgt tagccgcaaa
      661 gaaagtatca aa
//
LOCUS       DAZFWL020000526          671 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZFWL020000526 DAZFWL020000000
VERSION     DAZFWL020000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.532"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..506
                     /gene="rfbC"
                     /locus_tag="ACHKNP_RS28250"
                     /old_locus_tag="pgaptmp_005650"
     CDS             <1..506
                     /gene="rfbC"
                     /locus_tag="ACHKNP_RS28250"
                     /old_locus_tag="pgaptmp_005650"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HGU4707559.1"
                     /translation="FEPKVFGDERGFFMESFNQKVFEEAVGRKVEFVQDNHSKSSKGV
                     LRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVNLSAENKRQLWIPE
                     GFAHGFLVLSEIAEFVYKTTNYYHPESEGCIKWDDSFLMIDWPNKPIAISEKDKKGSS
                     ILRLNDY"
BASE COUNT          197 a           89 c          162 g          223 t
ORIGIN      
        1 tttttgaacc gaaagttttt ggtgatgagc gtggtttctt tatggaaagc tttaatcaga
       61 aagtttttga agaagctgta gggcggaagg ttgaatttgt tcaggataat cattctaaat
      121 catctaaggg tgtgttacgc ggactgcact atcagctgga accctatgct caaggtaaat
      181 tagttcgttg tgtggtcggt gaagtatttg atgtagcagt tgatattcgt aaatcgtcac
      241 ctacatttgg gaaatgggtt ggggtgaatt tgtctgctga gaataagcgt cagttgtgga
      301 tccctgaggg gtttgcgcat ggttttttgg tgttaagcga gatagcagag tttgtttata
      361 agacgacaaa ttattatcat cctgaatcag aaggttgcat aaaatgggat gattcttttt
      421 tgatgattga ttggccaaat aaaccgatag caatctcaga aaaagataaa aaaggctcat
      481 caattttgag gctaaatgac tattgatgtt gaaaaaaaaa cttcaaaaaa taaaggaata
      541 tcattcagta ttggagttgg caataattca gggtgcgaat gccatatttc ctgtgttggt
      601 attcccattt tttcttatta ccttagggga aaacatcttt tcaagtattg ctgttggtga
      661 agtactagca c
//
LOCUS       DAZFWL020000527          668 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZFWL020000527 DAZFWL020000000
VERSION     DAZFWL020000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.533"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>668
                     /locus_tag="ACHKNP_RS28255"
                     /old_locus_tag="pgaptmp_005651"
     CDS             <1..>668
                     /locus_tag="ACHKNP_RS28255"
                     /old_locus_tag="pgaptmp_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4707560.1"
                     /translation="HIRTITHRVTDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQY
                     KAEDMLREAIGGLASRPEGFVVYTTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLP
                     VIFEHPPEMVESGANLLMENLAMVNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSK
                     HANVEIGLALRSDRWAGADFWEEQGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVT
                     GRDR"
BASE COUNT          142 a          163 c          220 g          143 t
ORIGIN      
        1 catatccgga ccatcaccca tcgggtgacg gacaccaccc tgaaggtggt ggcagccgat
       61 ccgaatacgg tgtccggtat caagtccgtg gggacactga ttgatgaact gtggctgttt
      121 ggcaagcagt acaaggcgga agacatgcta cgtgaagcca tcggcgggct tgcctcccgt
      181 ccggaagggt ttgtggtgta tacgaccacc cagtcgaatg aaccgcccgc cggggtgttc
      241 agacagaaac tgcagtatgc ccgggatgtc cgtgacggca aaattcatga tccgcacttt
      301 ctgccggtga tatttgaaca ccctcctgaa atggtggaaa gcggggctaa cctgctgatg
      361 gaaaacctcg ccatggtcaa tccgaatctc ggctattcag tggatgaggc ctttctgtac
      421 cgggagtacc gtaaagcccg ggaagccggt gaagagacat tccgggggtt catgtcaaaa
      481 cacgccaatg tggaaattgg tcttgccctg cgctctgacc gctgggcggg ggctgatttc
      541 tgggaagagc agggccgttg tatcagcctg gacgatatcc tgcgtcgtgc tgatgtggtg
      601 acggtgggga ttgacggcgg agggctggat gatctgctgg ggatgtatgt gactgggcgt
      661 gaccggga
//
LOCUS       DAZFWL020000528          667 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZFWL020000528 DAZFWL020000000
VERSION     DAZFWL020000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.534"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(17..382)
                     /locus_tag="ACHKNP_RS28260"
                     /old_locus_tag="pgaptmp_005652"
     CDS             complement(17..382)
                     /locus_tag="ACHKNP_RS28260"
                     /old_locus_tag="pgaptmp_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4707561.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGHGRADDIIGL"
     gene            424..609
                     /locus_tag="ACHKNP_RS28265"
                     /old_locus_tag="pgaptmp_005653"
     CDS             424..609
                     /locus_tag="ACHKNP_RS28265"
                     /old_locus_tag="pgaptmp_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4707562.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
BASE COUNT          176 a          195 c          150 g          146 t
ORIGIN      
        1 ccccccccgg ggcgggtcac agtccgatga tatcgtctgc cctgccatga cctccggaca
       61 cctccggcag cgtcgggtcc ggcataccac ccgccgcttc acgagcagac ttttgtcgat
      121 ggcattcggt acagagcgtc cagagattcg tctcctcatt accaccaccg aactgaagtg
      181 caattcggtg atcgagttca ctgtcacaga ggtcaaccac acgaccacag agacagcact
      241 gcccggcatc cctgagccag atatgacgct tgagggaaac acgtgcactg ccactgacac
      301 gacgctgttc acccttcaga atattcaccc gtcgggtatt cagtgttttg attctgctct
      361 ggagtgtacg aagctcagcc atgtaaaatc cccgtcatat ggcaatcagt aaaggaaata
      421 aatatgtcat cgaaaaaccg gacccgcaga accacaaccc gcaatatccg tttccccaat
      481 cacatgattg aacagatcaa tatcgccctt gaacataaag gttccggtaa cttttcagcg
      541 tgggttattg aagcctgcag aagaaggctg tcaacagagc gttcgggtat gaattacata
      601 attaagtaac atggtgttca cagaacacac agttaccgga cacatcagtt ttccattcgt
      661 cccccgg
//
LOCUS       DAZFWL020000529          653 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZFWL020000529 DAZFWL020000000
VERSION     DAZFWL020000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.535"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..211)
                     /locus_tag="ACHKNP_RS28270"
                     /old_locus_tag="pgaptmp_005654"
     CDS             complement(<1..211)
                     /locus_tag="ACHKNP_RS28270"
                     /old_locus_tag="pgaptmp_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU4707563.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDELQR"
BASE COUNT          222 a          127 c          136 g          168 t
ORIGIN      
        1 ggcgctgtaa ttcatcgccc tgccatatcg tcaggtaggc cattttgtcc agcacctgcg
       61 caaattcaag ggagagaaac tgctggctga ccagggagag atcgagggaa aagagcgaag
      121 agagtgactg gttcagatga aaggaaacca ccgcaaaagc gtccggcggc aggccgtcca
      181 cttccagtgt gaaacgtaat ccggttgaca tagcaacctc cggtaaaatg aataatgatc
      241 cgtaccgtac aactgcggat aatgtgataa gcgggacgac ttcagacgtt attagggaag
      301 gaacagaaaa tgctatggtt atgtgagaat tttgtttaaa tattacttta cagaaacggg
      361 tgtctgttca gtcaaaaaaa taatcacaag ctcaacctct tgtttcttgg cgttattcag
      421 gacaggtacg catatcacaa cgagtaacaa aaacgcaaag ttaaaccaat attcataata
      481 tatttttatt cacagcaata aataatccaa ttaacattat taaaatgaat gattgcataa
      541 gaaatatatg ctaccgctcc accctgataa catgatctgc acattgcaca tcttaacgtt
      601 gttaaatact ggctcaatag tggatatttt aaattaggaa tttatcaaat tca
//
LOCUS       DAZFWL020000530          652 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZFWL020000530 DAZFWL020000000
VERSION     DAZFWL020000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.536"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>652
                     /locus_tag="ACHKNP_RS28275"
                     /old_locus_tag="pgaptmp_005655"
     CDS             <1..>652
                     /locus_tag="ACHKNP_RS28275"
                     /old_locus_tag="pgaptmp_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HGU4707564.1"
                     /translation="IIKKYGITEGMNPKPRNARISKELLLLAVDRQIEWAKKHFITKD
                     VLENVVSKCDLSSIFNVNKVLQNTILEFVHEINNISSARWMSKSEKNNKQKEAIEKFK
                     KEVSHMNGGQQFIWGFDKVIQEGLSGLIELSIDINDSTNHRDKSSLSPDGRAVLHFLG
                     TIWNMAMGAVPGYNALSGVSSILHSAIVKESSNICDYIQGAVRIGMDFVPGTRSDL"
BASE COUNT          221 a           80 c          140 g          211 t
ORIGIN      
        1 atataataaa aaagtatggt ataactgagg gtatgaatcc taaacctagg aatgccagaa
       61 tatctaaaga attgctttta ttagctgttg atagacagat tgagtgggcg aaaaaacatt
      121 ttataacaaa agatgtattg gaaaatgttg tgtcaaaatg tgatttatca tctatcttta
      181 atgttaataa agtgcttcag aatactattc ttgagtttgt ccatgaaatt aataatatat
      241 catctgctcg ctggatgtca aaatcagaaa agaataataa acaaaaagag gcaatagaaa
      301 agttcaaaaa agaagtatcc catatgaatg gcgggcagca gtttatttgg gggtttgata
      361 aggttattca agaaggctta agtggattga ttgagttaag tatcgatatt aatgatagta
      421 caaatcatcg tgataagtct tctctttctc ctgatgggag agctgtgtta cattttttag
      481 gtacaatttg gaatatggcg atgggagctg tacctggtta taatgcattg tctggtgtaa
      541 gtagcatttt acatagtgct atagtaaaag aatctagcaa tatctgtgat tatattcagg
      601 gggctgtacg tattggaatg gactttgttc caggcactcg ctctgactta ca
//
LOCUS       DAZFWL020000531          650 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZFWL020000531 DAZFWL020000000
VERSION     DAZFWL020000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.537"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..427)
                     /locus_tag="ACHKNP_RS28280"
                     /old_locus_tag="pgaptmp_005656"
                     /pseudo
     CDS             complement(1..427)
                     /locus_tag="ACHKNP_RS28280"
                     /old_locus_tag="pgaptmp_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(436..>650)
                     /locus_tag="ACHKNP_RS28285"
                     /old_locus_tag="pgaptmp_005657"
     CDS             complement(436..>650)
                     /locus_tag="ACHKNP_RS28285"
                     /old_locus_tag="pgaptmp_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGU4707565.1"
                     /translation="SPVIAVMLGGEAVLGLLARYASQLNAFAISLTVKSALAFLILII
                     YFAPILAERVMPLSFFPEQLQLYIEK"
BASE COUNT          219 a          123 c          114 g          194 t
ORIGIN      
        1 aaacaatata aagaattgct ttaacaaatt cttttacggt ttttaagcca aatatacgct
       61 ttaagccatt tactggattc aatgaatcaa acttaaattt aacagcttca gttgctaatt
      121 gcattttact ttgaaaccag gagacaagta ttgttgtgaa catgcaaacc agcaaaaaag
      181 gaatgttcgt tttcaaccaa ccaaccccca tagctttaaa atagtcccaa atatctggtt
      241 tagcgttatg atcaaggatg tattcaagaa tcgacataat gtgatgcaca tcaaaaacat
      301 atccgagata taaagtaccc acaagcatta ttacagagat ggttaagtct ctactttata
      361 gaaattgccc ttttttagaa gcatcctgga gtttcttttg tgttggcttt tcagttttat
      421 tggccatgaa acactctatt tctcaatgta tagttgcaat tgttctggga aaaaggataa
      481 cggcattact cgttcagcca gtattggggc aaaataaata attaatatta aaaatgccag
      541 cgcacttttg acagtcagtg aaatggcaaa agcatttagt tgtgaagcat aacgtgccag
      601 caatcccagg acagcctcac cgcccagcat aacggcaatc acggggcttg
//
LOCUS       DAZFWL020000532          644 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZFWL020000532 DAZFWL020000000
VERSION     DAZFWL020000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.538"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..487
                     /locus_tag="ACHKNP_RS28290"
                     /old_locus_tag="pgaptmp_005658"
     CDS             <1..487
                     /locus_tag="ACHKNP_RS28290"
                     /old_locus_tag="pgaptmp_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4707566.1"
                     /translation="RHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDFYISYLRRS
                     KNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCIFALSKLAM
                     ALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLLDEYIRATT
                     E"
     gene            459..>644
                     /locus_tag="ACHKNP_RS28295"
                     /old_locus_tag="pgaptmp_005659"
     CDS             459..>644
                     /locus_tag="ACHKNP_RS28295"
                     /old_locus_tag="pgaptmp_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707567.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMN"
BASE COUNT          191 a          138 c          159 g          156 t
ORIGIN      
        1 caggcattta agtctgaccg atcgccagct tcgcagattg cagaaattgc cgggtgcccc
       61 gatatcaaat aagcgagggc aactggatct ggatgcctgg cgcgattttt acatatcgta
      121 tctgaggaga agtaaaaacg atgtgcctga tggcgatagc gaagacgact atgaggaaaa
      181 attgcttatt gccagatggg aactgacagc agaacaggct gttacacagc agttaaaaaa
      241 tgaggtgtca aaaggaaaac ttattgacac cgggttctgt atttttgccc tcagtaagct
      301 ggcaatggcg ttatccagta cgcttgattc catcccttta tccatgcaac gacagtttcc
      361 tgatttaaca ccgcgccatc ttgaccatct gaaaaccctt attgcgaagg gggcaaatca
      421 gtgtgcgcgg gcgggggata aattaccgga tttactcgat gaatatatca gagcaacaac
      481 tgaataatat gatgagtgct gtcacaacag cattacagcc cctgataagg gcattgccgg
      541 tgacgccagt tgaatgggct gatcaaaatt attatctgcc taaagaatct tcatatggtg
      601 agggagaatg gaaaacgctg ccattccaga tcgccatcat gaac
//
LOCUS       DAZFWL020000533          643 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZFWL020000533 DAZFWL020000000
VERSION     DAZFWL020000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.539"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>643
                     /locus_tag="ACHKNP_RS28300"
                     /old_locus_tag="pgaptmp_005660"
     CDS             <1..>643
                     /locus_tag="ACHKNP_RS28300"
                     /old_locus_tag="pgaptmp_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4707568.1"
                     /translation="ARKNGTYTDLPQNVDTAEDSVQPEPVKVTADEVNKIMQAANISQ
                     PDADKLLAASRGEFVEGISDPNDPKWVKGIETCDSVNQNQQESEQNDQKTEQNSPNAQ
                     QNEPETKQEPEKACTACGQTGGGSCPDCGAVISDATYQEIFDEENQPEVQENDPEEME
                     GTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNITSTSRAGIHLMIDLET"
BASE COUNT          215 a          151 c          170 g          107 t
ORIGIN      
        1 ttgcccgcaa aaacggaaca tacacggact taccgcaaaa tgtggatacc gctgaagatt
       61 ccgtacaacc ggagccggta aaagttaccg ctgacgaagt aaacaaaatt atgcaggcag
      121 ccaatatcag ccagcctgac gccgataaat tgcttgctgc atcacgtggt gaatttgttg
      181 aagggattag cgatccgaat gatccgaaat gggtgaaggg gattgaaacc tgcgattctg
      241 tgaaccagaa ccagcaagaa tcggaacaga acgaccagaa aacggaacaa aacagcccaa
      301 atgcgcaaca aaacgagcca gaaacgaaac aggagccgga aaaagcctgc accgcctgcg
      361 gtcagaccgg cggcggtagc tgccctgatt gtggtgcggt gataagtgac gcaacatacc
      421 aggaaatatt cgatgaagag aatcagcctg aagttcagga aaatgatccg gaggaaatgg
      481 aaggcactgc gcatcagcac aaggagaaca ctggcggcaa tcagcatcat gccagcgata
      541 gtgaaactgg cgaggcgtca gatcccttaa ttaaggcgaa cggtcatcat aatatcacat
      601 ccaccagcag agcggggatt catctgatga tcgaccttga aac
//
LOCUS       DAZFWL020000534          633 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZFWL020000534 DAZFWL020000000
VERSION     DAZFWL020000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.540"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            5..118
                     /locus_tag="ACHKNP_RS28305"
                     /old_locus_tag="pgaptmp_005661"
     CDS             5..118
                     /locus_tag="ACHKNP_RS28305"
                     /old_locus_tag="pgaptmp_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HGU4707569.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            187..420
                     /gene="ydfK"
                     /locus_tag="ACHKNP_RS28310"
                     /old_locus_tag="pgaptmp_005662"
     CDS             187..420
                     /gene="ydfK"
                     /locus_tag="ACHKNP_RS28310"
                     /old_locus_tag="pgaptmp_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HGU4707570.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKM"
BASE COUNT          237 a          128 c           91 g          177 t
ORIGIN      
        1 ctatatgaac agcatactga taatcacttc tctccttatc atattcagca tttttagtca
       61 tgccctaata aaattaggga ttggcatatc caataaccca gacaaaaccg atgtataagt
      121 caacatatcc tgaatcagac atacaatatc gcaatgaaaa tcaataatat tttaaggaat
      181 atcttcatga aatcaaaaga caccctaaag tggttccctg cgcagcttcc tgaagtaaga
      241 attatcctag gggatgctgt agtggaagta gcaaaacagg gaagacctat caataccaga
      301 acattgcttg attacattga aggaaacata aagaaaaaat catggctgga taacaaagaa
      361 ttattacaaa cagcgatatc agttcttaaa gacaaccaaa atttaaatgg taaaatgtaa
      421 tataataaac ttactttttt atcatttttc cactttaaca acattttgct ccacttttcc
      481 acgaccaaac aacttgaaat ctggttaaaa taacacgcaa cactattctt cttccttgag
      541 tccgcccgga actcgaaaaa caaaccgagt taaagccatt tttcacaaaa tcgattttgg
      601 gtctcaccaa aattacgggg ttgcatacgc att
//
LOCUS       DAZFWL020000535          631 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZFWL020000535 DAZFWL020000000
VERSION     DAZFWL020000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.541"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>631
                     /locus_tag="ACHKNP_RS28315"
                     /old_locus_tag="pgaptmp_005663"
     CDS             <1..>631
                     /locus_tag="ACHKNP_RS28315"
                     /old_locus_tag="pgaptmp_005663"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGU4707571.1"
                     /translation="VRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETARSQNY
                     ALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSEN
                     VLDILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNID
                     EYINNNSEDKLIVFHLMGSHGPTYYLRYPEPHKYFKPTCDRSDIENCTHE"
BASE COUNT          211 a          102 c          129 g          189 t
ORIGIN      
        1 tgtaagggat ttttttgttt ctcctggcga gtttcgtcag actggaacgg atgcatcccg
       61 cacaataaat gaaaaacaaa agccggtaat tatgttttta gttgtcgggg agacagcaag
      121 aagtcaaaac tatgctctga atggatattc tcgtggtacc aatgatttta caaagaaata
      181 taatgaactt atatcctttc ataatgtgca gtcgtgtggc acatccacag ccatatctgt
      241 tccatgcatg ttttcagata tgaagcgaaa agagttcaat tcccgcaaag cggtaaattc
      301 ggaaaatgta ctggatattc tgtacagaac gggggtaaat ttactctgga ttgaaaatga
      361 cgggggatgt aaaggtgtat gtaagcgaat ccctacaatt aatattgagc catcaaattc
      421 agataataca ttatgcaaga aaaactcatg ttatgatgag gttatgctaa aaaatattga
      481 tgagtatata aataataact cagaggataa acttatcgta tttcatttga tgggtagtca
      541 tggtcctacc tactatctcc gttatcctga gccacataaa tattttaaac caacatgtga
      601 tcgtagtgat atcgaaaatt gtacgcatga g
//
LOCUS       DAZFWL020000536          628 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZFWL020000536 DAZFWL020000000
VERSION     DAZFWL020000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.542"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..465
                     /locus_tag="ACHKNP_RS28320"
                     /old_locus_tag="pgaptmp_005664"
     CDS             61..465
                     /locus_tag="ACHKNP_RS28320"
                     /old_locus_tag="pgaptmp_005664"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4707572.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPTTSPRSQPVAAATPESLN
                     ISCEVTFRHGSLRLNGAISENILNLLIRELKR"
     gene            462..>628
                     /gene="tnpB"
                     /locus_tag="ACHKNP_RS28325"
                     /old_locus_tag="pgaptmp_005665"
     CDS             462..>628
                     /gene="tnpB"
                     /locus_tag="ACHKNP_RS28325"
                     /old_locus_tag="pgaptmp_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4707573.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKL"
BASE COUNT          175 a          165 c          150 g          138 t
ORIGIN      
        1 tctggcagat cctgaaattc ctgagagaat agtggacacc aaatatggtg gacgctatcc
       61 atgaaatcat taaccgcagt gcgtaaaaaa agccctaatt atcccgttga gttcaaaatc
      121 aaaatggttg aactctcgca tcgaccagag atctccgtag cgcaactcgc tcgtgagcat
      181 gggatcaacg ataatttgct gttcaagtgg cgccagtact ggcgcgaagg aaaactacgt
      241 cctccttcaa caacagaaaa caacgtgcct gagctgctcc cgataacact tgatgccgaa
      301 gatgttgtcc ctacaacctc cccccggtca caacctgtag ctgctgcgac acctgaatca
      361 ctcaatatca gctgtgaagt gacgttccgg cacggatcac tccgtctgaa tggtgccatc
      421 agcgaaaata tcctgaacct gctgatacgg gagctcaaac gttgatccca ttaccatcag
      481 ggacaaagat ctggctggtc gctggcatca ccgatatgag aaacggcttc aacggcctgg
      541 cggcaaaggt gcagacgacg ctgaaagacg atccgatgtc aggtcacgtt tttatcttcc
      601 gtgggcgtaa tggcagtcag gtaaagct
//
LOCUS       DAZFWL020000537          625 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZFWL020000537 DAZFWL020000000
VERSION     DAZFWL020000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.543"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            43..>625
                     /gene="rfaY"
                     /locus_tag="ACHKNP_RS28330"
                     /old_locus_tag="pgaptmp_005666"
     CDS             43..>625
                     /gene="rfaY"
                     /locus_tag="ACHKNP_RS28330"
                     /old_locus_tag="pgaptmp_005666"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HGU4707574.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRID"
BASE COUNT          228 a           84 c          109 g          204 t
ORIGIN      
        1 aagaaaaatg cttctaaaga tttaaggtta aaataattca ttatgatata taataaaacg
       61 ataaatgggt tgaaagtttt cataaaagat aatgatccct tttatgagca agttctaaat
      121 gactttttaa cttgcagggt taaaacatta aaagtttttc gttcgattga tgatactaaa
      181 gtcattctta ttgacactgc tcgtggacct cttgtactta aagtttatgc gccaaagcat
      241 aaaatgaccg aacgtttctt aaaatcttgc ataaaaaaag attattatga aaatttaatt
      301 tatcaaaccg atcgggtacg tggtgaaggc atccaatcaa ttaatgacta ttttttgcta
      361 gctgaacgta aaacattaaa tttcgctcat tattatatta tgctcattga gtatattgaa
      421 ggtgtcgggt taaatgagta tttggaaatt tctgaagatc tgaaggatca attatcagaa
      481 tcgataaaag agttgcatca gcatggaatg gtgtctggcg atcctcataa aggaaatttt
      541 atagtatccg aaaaaggttt aagactaatt gacctttcag gtaaaaaaac aaccgctgtt
      601 ttaaaagcaa aagatcgcat tgatc
//
LOCUS       DAZFWL020000538          619 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZFWL020000538 DAZFWL020000000
VERSION     DAZFWL020000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.544"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>619
                     /locus_tag="ACHKNP_RS28335"
                     /old_locus_tag="pgaptmp_005667"
     rRNA            <1..>619
                     /locus_tag="ACHKNP_RS28335"
                     /old_locus_tag="pgaptmp_005667"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          142 a          138 c          201 g          138 t
ORIGIN      
        1 atggagccga ccttgaaata ccacccttta atgtttgatg ttctaacgtt gacccgtaat
       61 ccgggttgcg gacagtgtct ggtgggtagt ttgactgggg cggtctcctc ctaaagagta
      121 acggaggagc acgaaggttg gctaatcctg gtcggacatc aggaggttag tgcaatggca
      181 taagccagct tgactgcgag cgtgacggcg cgagcaggtg cgaaagcagg tcatagtgat
      241 ccggtggttc tgaatggaag ggccatcgct caacggataa aaggtactcc ggggataaca
      301 ggctgatacc gcccaagagt tcatatcgac ggcggtgttt ggcacctcga tgtcggctca
      361 tcacatcctg gggctgaagt aggtcccaag ggtatggctg ttcgccattt aaagtggtac
      421 gcgagctggg tttagaacgt cgtgagacag ttcggtccct atctgccgtg ggcgctggag
      481 aactgagggg ggctgctcct agtacgagag gaccggagtg gacgcatcac tggtgttcgg
      541 gttgtcatgc caatggcact gcccggtagc taaatgcgga agagataagt gctgaaagca
      601 tctaagcacg aaacttgcc
//
LOCUS       DAZFWL020000539          618 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZFWL020000539 DAZFWL020000000
VERSION     DAZFWL020000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.545"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..555
                     /locus_tag="ACHKNP_RS28340"
                     /old_locus_tag="pgaptmp_005668"
     CDS             <1..555
                     /locus_tag="ACHKNP_RS28340"
                     /old_locus_tag="pgaptmp_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4707575.1"
                     /translation="SRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTDIAPPDIPA
                     GFVAVFNSDEASWNLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSPEGEFQKWN
                     GTAWVKDAEAEKLFRIRKAEETKNSLMQMASEHIAPLQDAVDLDIATEEEASLLTAWK
                     TYRVLLNRVDTTVASDVEWPVAPQ"
BASE COUNT          178 a          132 c          160 g          148 t
ORIGIN      
        1 tcacggacca taaaaattta taatctactg gctggaacca atgagtttat tggtgaaggt
       61 gatgcataca ttccacctca tacaggtctg cctgcaaaca gtaccgatat tgccccgcca
      121 gatattccgg ctggcttcgt ggccgttttc aacagtgatg aggcatcgtg gaatctcgtt
      181 gaagaccatc ggggaaaaac cgtctatgac gtggcttccg gcgacgcgtt atttatttct
      241 gaacttggcc cattaccgga aaatgtcacc tggttatccc cggaagggga gtttcagaag
      301 tggaacggca cagcctgggt gaaagatgca gaagcagaaa aactgttccg gatccggaag
      361 gcggaagaaa caaaaaacag cctgatgcag atggccagtg agcatattgc gccacttcag
      421 gatgccgtag atttggatat tgcgacggag gaagaggcat cgttactgac tgcatggaag
      481 acatatcggg tattgttgaa tcgtgttgat acaacagtag catcggatgt tgagtggcca
      541 gtcgccccgc aataaaagga taaagccatc gatagaaata ttgatggctt catgtactct
      601 atttatacaa tacaacac
//
LOCUS       DAZFWL020000540          612 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZFWL020000540 DAZFWL020000000
VERSION     DAZFWL020000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.546"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..283)
                     /locus_tag="ACHKNP_RS28345"
                     /old_locus_tag="pgaptmp_005669"
     CDS             complement(<1..283)
                     /locus_tag="ACHKNP_RS28345"
                     /old_locus_tag="pgaptmp_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707576.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYYDNPTTALD
                     WSNMDAPGTVDGNYVNYSGFVYYNNTNGDFDTTFNGDTVNGTISTYYLNH"
BASE COUNT          206 a          114 c          111 g          181 t
ORIGIN      
        1 catgattcag gtaatatgta gagatagtcc cgttaacagt gtcaccatta aaggtggtat
       61 cgaaatcacc gtttgtgttg ttatagtaga caaagccact ataattcaca tagttaccat
      121 ctacagtacc cggtgcatcc atattgctcc agtcaagagc ggtagttgga ttgtcgtaat
      181 aagtccaggt gtcataagta gttgcattag cctgagaggt gatagccaga gtgcaagcca
      241 atgctaattg tgatactaca agtttctttt tccaggagtg catttgtcat ccctcctcag
      301 ggacgtaata agttgatcca tcaattatca atgcatagaa aacatattat gtttcgcgct
      361 aatgattatg ctgcgttaca gacgaatggt tcaattattc tttcccctaa gtccgaatgt
      421 ggacatatat aacctatgga gaaaaacacg catcaaattg ataaaagggc gatatttcaa
      481 catgttgcag ataatgttat ttttgataca tacgcgttat aaatatcaaa aacacaaacc
      541 agataaatat cttatcaata tacaacaaag tttgaattat tcaatttata gcagcacaac
      601 caaacgccaa aa
//
LOCUS       DAZFWL020000541          610 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZFWL020000541 DAZFWL020000000
VERSION     DAZFWL020000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.547"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..285
                     /locus_tag="ACHKNP_RS28350"
                     /old_locus_tag="pgaptmp_005670"
     CDS             <1..285
                     /locus_tag="ACHKNP_RS28350"
                     /old_locus_tag="pgaptmp_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707577.1"
                     /translation="IIDSRNISAPHELANILTREKILTTREKKGNLISLFDGFTLFHC
                     VGMITFLIHYLRTPEEKVENIFMLYGADKNNKLRRRLIYDALGIIQSQQE"
     gene            complement(473..>610)
                     /locus_tag="ACHKNP_RS28355"
                     /old_locus_tag="pgaptmp_005671"
     CDS             complement(473..>610)
                     /locus_tag="ACHKNP_RS28355"
                     /old_locus_tag="pgaptmp_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HGU4707578.1"
                     /translation="LAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADYVMSQIVN
                     K"
BASE COUNT          208 a          128 c           98 g          176 t
ORIGIN      
        1 attatagaca gcagaaacat atcagccccc catgaactgg caaatattct tacccgcgaa
       61 aaaatactta caacacggga aaaaaaagga aacctgataa gcctgtttga tggattcact
      121 ttattccatt gtgttggaat gataaccttt cttatccatt atctcagaac acctgaagaa
      181 aaagttgaaa atatatttat gttatatggt gcagataaaa acaataaact acgcagaaga
      241 ctgatttatg acgcactagg aataattcag tctcagcagg agtgaaacgt taaaacaggc
      301 aaattgtttt ttataaacag gagattgttg agtaatcccc tgtttataaa aatacgcttt
      361 cctgacgatc tgataattat cagctcaata catttgacat aaaagcggtt ccttaattac
      421 tactgctacg atcacatatc aaatcatgat cgtacaatat acattttcac tattatttat
      481 tcacaatttg agacatcaca taatccgccc atgcctgcat catcggtaca cgctgctcca
      541 gtagatcagt acgatgatat gccgcctcaa ccttattttt cagcgtatga gcgagcgccc
      601 tttccgccag
//
LOCUS       DAZFWL020000542          600 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZFWL020000542 DAZFWL020000000
VERSION     DAZFWL020000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.548"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          150 a          118 c          144 g          188 t
ORIGIN      
        1 aatagcattc agaaaaaatt tttattgtgc tgataaaata taccattagt gattagttta
       61 gatacctggc tttcaggcaa cttctttttg tctgtatata agggatgatt actaaggtgg
      121 tacattgata tcagttagct ttcccttttc gggttacttt atcaaagagt cctctttata
      181 gaagagcgca ttgtgagttt tccagattga cttaaacgtg tggcggcatc acaccgatca
      241 acacgatctg cagcaaagag caggtgtaac caggcccatc ttagcgctat gcacttttga
      301 gccactgagc ttgctaaccg tttacccggc tttcactgag ctaattacgg cgataatctg
      361 atgcccagta aaacggactt gatgtatgac gggtcctctt ttgcgggata gatatgggaa
      421 tatttcttaa tgcgaatggt acggttatgc gggggggaca aacatggttg cttgttctgc
      481 catttgttcc ttgttccttt ttataccgcc gccgctcccg gtcagcactg gggaataggg
      541 ggttgaggta tatctgctcc ttatgtcagg ggaacaggta atagctatat agcggaacgt
//
LOCUS       DAZFWL020000543          596 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZFWL020000543 DAZFWL020000000
VERSION     DAZFWL020000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.549"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..211)
                     /locus_tag="ACHKNP_RS28360"
                     /old_locus_tag="pgaptmp_005672"
     CDS             complement(<1..211)
                     /locus_tag="ACHKNP_RS28360"
                     /old_locus_tag="pgaptmp_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HGU4707579.1"
                     /translation="MSKDFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATAT"
     gene            complement(222..392)
                     /locus_tag="ACHKNP_RS28365"
                     /old_locus_tag="pgaptmp_005673"
     CDS             complement(222..392)
                     /locus_tag="ACHKNP_RS28365"
                     /old_locus_tag="pgaptmp_005673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707580.1"
                     /translation="MSLATTVKESKLQRRMYTQQALMYRQKGDREGVRVFLNAAKTEV
                     LNQRYFLGPCPF"
     gene            complement(468..>596)
                     /gene="kil"
                     /locus_tag="ACHKNP_RS28370"
                     /old_locus_tag="pgaptmp_005674"
     CDS             complement(468..>596)
                     /gene="kil"
                     /locus_tag="ACHKNP_RS28370"
                     /old_locus_tag="pgaptmp_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGU4707581.1"
                     /translation="HQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINLPWYRRK"
BASE COUNT          146 a          142 c          110 g          198 t
ORIGIN      
        1 tagttgccgt ggctttcaca taataccgat caccaatcaa cacaacttca tcgctgattg
       61 ataaaaacag gccattcagt aacggcttaa cgccttcaag aatgtcttcg cagcttctgt
      121 atttatattt accgaatgag ttgtactgat tctttggcgc gttcaagttc tcctgaatag
      181 ctgccagtct tgcgtaaaag tctttgctca tatgtttgtt ctcagaatgg gcatggccca
      241 aggaaataac gctgatttaa tacttcagtc tttgccgcat ttaaaaatac gcgaacacct
      301 tcacgatctc ccttctggcg atacattaac gcctgctgcg tgtacatgcg tctctgtaac
      361 ttgctctcct tcactgtggt tgcaagtgac atgaatatct ccttcgttac cgattaattc
      421 tttcatctga cgaatgaatt cttcgtctga ccagttatct gtgaaactca tttcctgcga
      481 taccacggaa ggttgatagc tgatttcatc gctttatttg cttcaagcca catttttgaa
      541 tcaccaataa atctggctat tactgctttg ttttgtgccg cacgaagcat ctggtg
//
LOCUS       DAZFWL020000544          595 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZFWL020000544 DAZFWL020000000
VERSION     DAZFWL020000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.550"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>595
                     /locus_tag="ACHKNP_RS28375"
                     /old_locus_tag="pgaptmp_005675"
     CDS             <1..>595
                     /locus_tag="ACHKNP_RS28375"
                     /old_locus_tag="pgaptmp_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4707582.1"
                     /translation="ALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVTLWYYDESD
                     RITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRVAVHYGYDDKGRLTGERQTVENPE
                     TGELLWQHETTHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGDTPLLEYTRDR
                     MHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQHLNS"
BASE COUNT          142 a          177 c          192 g           84 t
ORIGIN      
        1 gcgctggacc ggttggtaca gcagggcggc tttgacgggc ggacgcaacg ttatcattat
       61 gacctgaccg gaaaactcac gcagagtgaa gatgaggggc ttgtcaccct ctggtactac
      121 gatgaatcgg accgtatcac tcaccgcacg gtgaacggcg aaccggcaga gcagtggcag
      181 tatgatgacc acggctggct gacagacatc agccacctga gcgaaggcca ccgtgtcgct
      241 gtccactacg gctacgacga taagggccgc ctgaccggcg aacggcagac ggtggagaac
      301 ccggagacgg gggaactgct gtggcagcat gagacaacac acgcctacaa cgaacagggg
      361 ctggcaaacc gcgtcacgcc ggacagcctg ccgccggtgg agtggctgac ctacggcagc
      421 ggttatctgg cgggcatgaa gcttggcgac acgccgctgc tggagtacac gcgcgaccgc
      481 atgcaccggg agacggtgcg cagctttggc agcatggcag gcagtaatgc cgcatataaa
      541 ctgaccagca catacacccc cgcaggccag ttacagagcc agcacctgaa cagcc
//
LOCUS       DAZFWL020000545          581 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZFWL020000545 DAZFWL020000000
VERSION     DAZFWL020000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.551"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>581
                     /locus_tag="ACHKNP_RS28380"
                     /old_locus_tag="pgaptmp_005676"
     rRNA            <1..>581
                     /locus_tag="ACHKNP_RS28380"
                     /old_locus_tag="pgaptmp_005676"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          164 a          127 c          177 g          113 t
ORIGIN      
        1 ataatgggtc agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga
       61 aaccgagtct taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc
      121 catgggcagg ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa
      181 aattagcgga tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt
      241 ctccccgaaa gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt
      301 tcggcaaggg ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt
      361 tatcacggga gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac
      421 cgccagctaa ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag
      481 ccaggatgtt ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga
      541 gtcggcctgc gcggaagatg taacggggct aaaccatgca c
//
LOCUS       DAZFWL020000546          576 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZFWL020000546 DAZFWL020000000
VERSION     DAZFWL020000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.552"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..282)
                     /locus_tag="ACHKNP_RS28385"
                     /old_locus_tag="pgaptmp_005677"
     CDS             complement(<1..282)
                     /locus_tag="ACHKNP_RS28385"
                     /old_locus_tag="pgaptmp_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGU4707583.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQF"
     gene            complement(294..>576)
                     /locus_tag="ACHKNP_RS28390"
                     /old_locus_tag="pgaptmp_005678"
     CDS             complement(294..>576)
                     /locus_tag="ACHKNP_RS28390"
                     /old_locus_tag="pgaptmp_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707584.1"
                     /translation="CNTGKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDT
                     VLTAPTNLNSNKDLMAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          160 a          120 c           88 g          208 t
ORIGIN      
        1 aaactgcgct gtaacattcc cctgagatgc tgatctttcc aagaatcgca attgtttatc
       61 aatattattt ttagtaaagc agtaatattg atatagtcga aatgatgcct ctgaatcacc
      121 atgttgtgat ttatacttca gtttcatcag cttttcttca cttaaatcat aggtcattgc
      181 tgtagatccc atcacacttt cgttttcttt tattttcaca tcagcaccat gcaattgacc
      241 aataagagaa aaataaaaaa atagtaacaa gcatgctttc atacatctac aacctatctt
      301 atatcactgg gtttttctgg cgtaccttgc atattagaaa tggggtgccc agatggccca
      361 tatgttatcc aattaccaag gtcttttgtt gttatagcca ttagatcctt atttgaatta
      421 aggttcgttg gtgctgttag cactgtatcc atttttaact taccattagt tcctcgcccc
      481 caccacgtcc atagcgtgtc aggtcctatt acatttgtat gtaactcttt agctaattgg
      541 ctagcaaatg aattttgtcc tttacctgta ttacaa
//
LOCUS       DAZFWL020000547          573 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZFWL020000547 DAZFWL020000000
VERSION     DAZFWL020000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.553"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>573
                     /locus_tag="ACHKNP_RS28395"
                     /old_locus_tag="pgaptmp_005679"
     CDS             <1..>573
                     /locus_tag="ACHKNP_RS28395"
                     /old_locus_tag="pgaptmp_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU4707585.1"
                     /translation="LYIRNNGYYCYRDPRTGKEFGLGRDRRIAITEAIQANIELFSGH
                     KHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAIRRGLPDAPLE
                     DITTKEIAAMLSGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPVAATRAAKSEV
                     RRSRLTADEYLKIYQAAESSPCWLRLAMELA"
BASE COUNT          201 a          124 c          135 g          113 t
ORIGIN      
        1 ctttatataa gaaacaatgg atattactgc tacagggacc caaggacggg taaagagttt
       61 ggattaggcc gagacaggag gatagcaatc actgaagcaa tacaggccaa cattgagtta
      121 ttttcaggac acaaacacaa gcctctgaca gcgagaatca acagtgataa ttctgttacg
      181 ttacattcat ggcttgatcg ctacgaaaaa atcctcgcca gcagaggaat caagcagaag
      241 actctcataa attacatgag caaaattaaa gcaataagga gggggctgcc tgatgctcca
      301 cttgaagaca tcaccacaaa agaaattgcg gcaatgctca gtggatacat agacgagggc
      361 aaggcggcgt cagccaagtt aatcagatca acactgagcg atgcattccg agaggcaata
      421 gctgaaggcc atataacaac aaacccggtc gctgccactc gcgcagcaaa atcagaggta
      481 aggagatcaa gacttacggc tgacgaatac ctgaaaattt atcaagcagc agaatcatca
      541 ccatgttggc taagacttgc aatggagctg gct
//
LOCUS       DAZFWL020000548          571 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZFWL020000548 DAZFWL020000000
VERSION     DAZFWL020000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.554"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>571)
                     /locus_tag="ACHKNP_RS28400"
                     /old_locus_tag="pgaptmp_005680"
     CDS             complement(<1..>571)
                     /locus_tag="ACHKNP_RS28400"
                     /old_locus_tag="pgaptmp_005680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HGU4707586.1"
                     /translation="KIGQRVYTVVSEMSIDAWITKEPQPFVYRRSGAYQKLTIGSDWG
                     QLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGITNKASSYGVPPD
                     KPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIATCNQLLKSLYYDV
                     EVLFDWINDGQKFESIHPKGIIPEKIITKR"
BASE COUNT          171 a          131 c          108 g          161 t
ORIGIN      
        1 tcttttggtg ataatttttt ctgggataat acctttagga tgtatggatt caaacttctg
       61 accatcatta atccagtcga aaagcacttc aacatcataa taaagggatt tcagtagttg
      121 attacatgtc gcgatatgaa catcagtaat aatccctcca ttagtgacat agccaaataa
      181 atcgttgcac gcagcatcaa tccaaaactc gacctcgtca ttttcactaa cgagagaaag
      241 gtcaaccacc catttacctg gtttatctgg cggtacgcca taagatgatg ctttattcgt
      301 gatgccgcag atgggtgaac cattcttatc cacaataaga ccttcgccac cgatatcaac
      361 gagagcgacg agtttgtgtc taaatttatc tgctggacgt ttaccataaa ctcgcatcca
      421 ggcacagtca aacagttgac cccagtctga gccaatggtg agtttctggt aagcgccgct
      481 acggcgataa acaaaaggct gcggttcttt tgttatccat gcatcaatcg acatttcaga
      541 gacaacagta taaacacgct gacctatttt a
//
LOCUS       DAZFWL020000549          567 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZFWL020000549 DAZFWL020000000
VERSION     DAZFWL020000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.555"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            59..>567
                     /locus_tag="ACHKNP_RS28405"
                     /old_locus_tag="pgaptmp_005681"
     CDS             59..>567
                     /locus_tag="ACHKNP_RS28405"
                     /old_locus_tag="pgaptmp_005681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGU4707587.1"
                     /translation="MNSNYMPFTQRDSLGRYYTKESISALLVSQMKAEKVNNIIDLAS
                     GEGSLTYAALDRWKNAEAYSLDIESRMSKKVCDNLTHIVTDALVHSFPEMLARHQGNF
                     DVAVCNPPFTLPEWRDDYFKIISEIGADKYISVSKYVPAEIIFISQVIRFLKKGGEAG
                     IILPDGIFTA"
BASE COUNT          183 a           92 c          117 g          175 t
ORIGIN      
        1 tggttcttgg gtagccgtaa cgcctagggc ggtagcgtac ttaagatgag tataaaaaat
       61 gaacagtaac tatatgccat tcacacaacg agatagtctc ggaagatact atacaaaaga
      121 atccataagt gcattattgg tttctcagat gaaagctgaa aaagtcaata atattattga
      181 tttagcttct ggtgagggga gcttaaccta tgctgcttta gaccgatgga agaatgcaga
      241 agcatattct ttggatatag aatctcggat gtcaaaaaaa gtatgtgata atcttactca
      301 tattgtaaca gatgcccttg ttcattcatt tcctgaaatg cttgcccgac atcaaggaaa
      361 ttttgatgtt gcagtatgta atcctccatt cactctccct gaatggaggg atgattattt
      421 taaaatcatt agtgagattg gcgcagataa atatatatct gtctcaaaat atgtcccagc
      481 agagataata tttatatctc aagtcatcag gtttcttaaa aagggcggtg aggctggaat
      541 aattttacct gatggtatat ttacagc
//
LOCUS       DAZFWL020000550          566 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZFWL020000550 DAZFWL020000000
VERSION     DAZFWL020000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.556"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..185)
                     /locus_tag="ACHKNP_RS28410"
                     /old_locus_tag="pgaptmp_005682"
     CDS             complement(<1..185)
                     /locus_tag="ACHKNP_RS28410"
                     /old_locus_tag="pgaptmp_005682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4707588.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAITAEGDVVVSGATEP"
     gene            445..>566
                     /locus_tag="ACHKNP_RS28415"
                     /old_locus_tag="pgaptmp_005683"
     CDS             445..>566
                     /locus_tag="ACHKNP_RS28415"
                     /old_locus_tag="pgaptmp_005683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU4707589.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPE"
BASE COUNT          199 a          124 c           89 g          154 t
ORIGIN      
        1 tccggctcag ttgcaccact gaccaccaca tcaccttctg ctgtaatcgc ctgcatcaga
       61 gtataagggg ttatggccac cggactacca aacggctgcc agccctcttt cagtttatgt
      121 gtcagctttt ccgcaagatc tgatggcgac gccgccctga caacatcata gtgtttaaat
      181 gccatggttc tttccaccat ctgaaaaata attctttaaa atacctgaca tgtaatacag
      241 aaaaaacaca aaaccatacc ttaaataaaa acctgattat caagcagata tgcatggata
      301 aactacaaga cgagatataa accaccctgt atttaaataa acaataaaca acatcagaaa
      361 aataattctg ctctatggtt taattcaaaa atatcattta tacttttcag aacatcacca
      421 gcaaggcata aacaaggaaa gtaaatgaag tggattgtga ttgatacagt tatccagcca
      481 tcatgcggaa tatctttttc agtcatatgg agtaaaataa aattaataat ctggtatcaa
      541 tcggatgctt tcttacctcc tgaaag
//
LOCUS       DAZFWL020000551          558 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZFWL020000551 DAZFWL020000000
VERSION     DAZFWL020000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.557"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          174 a          120 c          111 g          153 t
ORIGIN      
        1 acttcagcta ccgtcgtttc atctctccca cgaaccggag ccgcaacttg ttgcatcaca
       61 gggctgacaa gtgaactgaa atccgcacct taatattctg actggctgcg tacattcatt
      121 gcatgaagcg gccggagcgg ctgagctgaa ggtccgttgt gagcgagaag cggaagtccg
      181 aaatggtgct tattactata ggggagtgat cgtctatcgg ggtggggtca gtctaattaa
      241 cgaccctgga gacgtccaga ttatccttag cgattcaaac ctttttacga gacccaaaga
      301 tttgatactc aaaatctcag acataaacca tctaaacata cggcttacta tcatgatgat
      361 aggggaacgg ctagcaaagg gtcaaattat cttgataact aataaaagtt ctttacatcc
      421 ataaaatcca tgatgtattt atctaatcct ttgcgattga aaaaaccgaa actttcaaag
      481 tcttttatta ttttccaatc actaaatacg tatgccaaac ataccaaatg aagaaatttc
      541 acaggcatag tataattt
//
LOCUS       DAZFWL020000552          545 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZFWL020000552 DAZFWL020000000
VERSION     DAZFWL020000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.558"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>545
                     /locus_tag="ACHKNP_RS28420"
                     /old_locus_tag="pgaptmp_005684"
     CDS             <1..>545
                     /locus_tag="ACHKNP_RS28420"
                     /old_locus_tag="pgaptmp_005684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HGU4707590.1"
                     /translation="SFTPFHNNTVNIAPNEKKFISLTEPDKLSFNIDVFREALEVKNS
                     YKLSKFTWETYGDIVVSPEDRISHLELDGFNYFSQPELDAQISDTFSYLYDNFQIADG
                     DVHIKLLHLDRETKQITPHRIILQKYFIDSFAKTSISDRENNIYPVICDSPDHFTNDI
                     YRHPFRIVLGNKTLYPSEELI"
BASE COUNT          195 a           90 c           81 g          179 t
ORIGIN      
        1 agcttcactc cttttcataa taatactgtg aatatcgccc ctaatgaaaa aaaatttatt
       61 tccctaacag agccagataa gttgtcattc aacattgatg tttttagaga agcattagag
      121 gtaaaaaata gttataagtt aagtaaattt acatgggaaa cgtatggtga tatcgtggta
      181 tcacctgaag atagaatttc tcatctggaa ttagatggat ttaattattt ttcacaaccc
      241 gagttagatg ctcaaatatc agacaccttt tcatatctat atgataattt tcagattgct
      301 gatggtgatg ttcatattaa gcttcttcat ctcgatagag aaacaaagca aataacgcca
      361 catcgtatta tccttcaaaa atactttata gactcatttg ctaaaacaag tatctctgac
      421 agagaaaaca atatatatcc tgttatttgt gattcacctg atcacttcac aaatgatata
      481 tataggcatc catttaggat agttctcggg aataaaacat tgtatccatc agaagaactt
      541 ataaa
//
LOCUS       DAZFWL020000553          543 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZFWL020000553 DAZFWL020000000
VERSION     DAZFWL020000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.559"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            259..>543
                     /gene="ecpR"
                     /locus_tag="ACHKNP_RS28425"
                     /old_locus_tag="pgaptmp_005685"
                     /gene_synonym="matA"
     CDS             259..>543
                     /gene="ecpR"
                     /locus_tag="ACHKNP_RS28425"
                     /old_locus_tag="pgaptmp_005685"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HGU4707591.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNI"
BASE COUNT          185 a           92 c           94 g          172 t
ORIGIN      
        1 tcagtttttc taaatattga cttaaccatt gaattccttt tccgttcaca tattgacact
       61 catcgggaaa aaaacataaa tttaagccca atcgaaaata attaaactta atctcgttta
      121 acctttattg atatgtacta cgtatcttat ttacttccgg tttactaacg aaactgaatg
      181 cacctgtaaa aattacaggt ttggaaagta gtgacatggc aaagtgatta cagcagggac
      241 tatgaggtta aaaaccatat ggaatgtcaa aaccgttctg ataaatacat ctggtctccc
      301 catgacgcct acttctataa aggactatct gaactgattg tggatatcga cagattaatt
      361 tatctatcgt tggagaaaat tagaaaagat ttcgtgttta tcaatctcaa tacggattct
      421 ttatctgaat ttataaaccg tgataatgaa tggttatccg cggtaaaggg gaaacaggtc
      481 gtattgattg cggccagaaa gtcagaagcc ttagcaaatt attggtatta caacagcaat
      541 att
//
LOCUS       DAZFWL020000554          527 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZFWL020000554 DAZFWL020000000
VERSION     DAZFWL020000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.560"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            235..>527
                     /locus_tag="ACHKNP_RS28430"
                     /old_locus_tag="pgaptmp_005686"
     CDS             235..>527
                     /locus_tag="ACHKNP_RS28430"
                     /old_locus_tag="pgaptmp_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707592.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKA"
BASE COUNT          161 a          102 c          159 g          105 t
ORIGIN      
        1 agaataaaaa aatcccgcag agttgcggag ctgcgggaag agatacaaag attaactata
       61 gagaattatt cttatgtcac gacaaaaaaa ttaacgcaga gaaattatta gcgccacagt
      121 cagtttgtga aaatgtgaag atattcagaa tttttattca gtgatgatac aggcatcctc
      181 cgggatgcct gttgtttttg tgcgtgaaca gttatcacag taaagggtga gacaatgggc
      241 aaaggtggcg gcaaggcgca cacgccggta gaggcaaagg acaatcttaa gtccacgcag
      301 atgatgagcg tgattgatgc gattggtgag ggaccgatag aaggtccggt gaagggactg
      361 cagagtattc tggtgaacaa aaccccactg acggacacgg acggcaatcc cgtgatacac
      421 ggtgtgaccg cggtctggcg tgccggggag caggagcaga caccaccgga aggctttgag
      481 tcctccggag ctgaaaccgg actgggcgtg gaagtgacga aggcaaa
//
LOCUS       DAZFWL020000555          518 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZFWL020000555 DAZFWL020000000
VERSION     DAZFWL020000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.561"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            151..507
                     /gene="grlR"
                     /locus_tag="ACHKNP_RS28435"
                     /old_locus_tag="pgaptmp_005687"
     CDS             151..507
                     /gene="grlR"
                     /locus_tag="ACHKNP_RS28435"
                     /old_locus_tag="pgaptmp_005687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HGU4707593.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNLITGGDIASVYQGVL
                     SEEEDIILHVHRYNHEIPSVLNIEQDYQLVIPKKVLSNDSNVTLHCHVRGNEKLFVDV
                     YARFIEPLIIKNTEIS"
BASE COUNT          184 a           66 c          106 g          162 t
ORIGIN      
        1 accacggaaa acaacaggtt ggaatatgag tatatttaat gatatattac attgcaatct
       61 ggagaaaaag aaaggtctcc gttattcttg atatcgctta tggatagaac aaattgaaag
      121 gagtgaggtt agtatgaaac tgagtgagtt atgattatga aggatggcat ctatagcatt
      181 atctttatta gcaatgaaga ttcctgtggg gaaggtatac tgattaaaaa tggaaatttg
      241 atcactggcg gagatattgc ttctgtgtat cagggggtcc tgtctgaaga ggaggacatc
      301 atacttcatg tccatcgcta taatcatgaa attccctcgg tgctaaacat tgaacaagat
      361 tatcaattag ttatccctaa aaaagtactg agtaatgata gtaatgtcac attacattgc
      421 catgtaagag gaaatgaaaa attgtttgtt gatgtttatg ccagatttat agaaccatta
      481 attattaaaa acacagaaat atcataagtt ttatttaa
//
LOCUS       DAZFWL020000556          518 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZFWL020000556 DAZFWL020000000
VERSION     DAZFWL020000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.562"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..416)
                     /locus_tag="ACHKNP_RS28440"
                     /old_locus_tag="pgaptmp_005688"
     CDS             complement(<1..416)
                     /locus_tag="ACHKNP_RS28440"
                     /old_locus_tag="pgaptmp_005688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4707594.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAAS"
BASE COUNT          116 a          159 c          133 g          110 t
ORIGIN      
        1 gaggcagccg aacgggcaaa cagggccgca ttatccgaaa atgcggtgcc tgtccgctgg
       61 ctgatatcca tcagcacttt ctgtgatgac gcaaattcat ccgatgactg cgacgcctgt
      121 ttcagacggg cattcacgga gctccattca tccgccagcg aaatcaggtg tccggtggca
      181 aaggcaccgg caaacgcacc ggtcattccg acagccgaag cgcggatttc cgtcaactgg
      241 ctgtgcagct cagccagagc ccggcgctgc tccctggctg ccgcagcagc ctgacgtccg
      301 ccattctgca gggtccggta atattcactg cccatacggg acgcccgctg gatctccgac
      361 tggaatgact gtgaatttgc cgaaattttg ataatcagtt cacgtaacgt cgccattcac
      421 ctttctccgg gcaaaaaaaa cctgccacag caggttttca tcattattta tgacattgct
      481 gcaaggctca gcgcgtcttc cagcgccgca aacggatc
//
LOCUS       DAZFWL020000557          516 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZFWL020000557 DAZFWL020000000
VERSION     DAZFWL020000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.563"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..516
                     /locus_tag="ACHKNP_RS28445"
                     /old_locus_tag="pgaptmp_005689"
                     /pseudo
     CDS             1..516
                     /locus_tag="ACHKNP_RS28445"
                     /old_locus_tag="pgaptmp_005689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          166 a           80 c          134 g          136 t
ORIGIN      
        1 aaccggaacc ggtactacga tccgttgcag gggcggtata tcactcagga cccgataggg
       61 ctggaggggg ggatggagtc tgtatgcgta cccgctgaat ccggtgaatg gtattgatcc
      121 attagggtta agtcccgcag atgtagcgct aataagaaga aaagatcaac taaaccatca
      181 aagagcatgg gatatattat ctgatactta tgaagatatg aagagattaa atttaggtgg
      241 gactgatcaa tttttccatt gtatggcatt ttgtcgagtg tctaaattaa atgacgctgg
      301 tgttagccga tcggcgaaag ggctgggtta tgaaaaagag attagagatt acgggttaaa
      361 tctgttcggt atgtacggca gaaaagtaaa gctatcccat tctgaaatga ttgaagataa
      421 taaaaaagac ttggctgtaa atgaccatgg gttgacatgt ccatcaacaa cagattgctc
      481 agatagatgt agtgattata ttaatccaga gcataa
//
LOCUS       DAZFWL020000558          509 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZFWL020000558 DAZFWL020000000
VERSION     DAZFWL020000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.564"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>509)
                     /locus_tag="ACHKNP_RS28450"
                     /old_locus_tag="pgaptmp_005690"
     CDS             complement(<1..>509)
                     /locus_tag="ACHKNP_RS28450"
                     /old_locus_tag="pgaptmp_005690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4707595.1"
                     /translation="TWQGRKYEAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAE
                     DLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFV
                     LATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCM
                     RGCELRRNVG"
BASE COUNT           99 a          191 c          125 g           94 t
ORIGIN      
        1 ccgacattcc tgcgcagttc acacccgcgc atgcacttgc tgcatctgtc cttacggata
       61 tccgtggtgg ggttgtcgaa ctcatccgcc accgcaggac cgttataccc gcattcatct
      121 ccccggtaat cccacataca ggtgttcgcc agcatgatgc gaccgggaaa cagcgcccca
      181 tccgtctccg tcggtgtagc cagcacaaac gaggccgtca tggctgtcag ctgcgacatc
      241 tgctccacca cccagcggtc actcagctcc tgctccgggt ccgcctccgg attgcccgca
      301 acgaaattca ccgcatccag aaaacgggca tacacccggc ggcggaccac cgtggccccc
      361 accagactct gcaggtcctc cgccatcccg gtgacaaggc caaacagatt ggacaccgtc
      421 agcgacgggc gggcactgct gcccttcccg ttcatctcaa agccgctgcc gtcaatcggg
      481 tatgcctcat acttacgccc ctgccaggt
//
LOCUS       DAZFWL020000559          507 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZFWL020000559 DAZFWL020000000
VERSION     DAZFWL020000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.565"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..401
                     /locus_tag="ACHKNP_RS28455"
                     /old_locus_tag="pgaptmp_005691"
     CDS             <1..401
                     /locus_tag="ACHKNP_RS28455"
                     /old_locus_tag="pgaptmp_005691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707596.1"
                     /translation="SINELIRKQKDIERMHTLISKVPGGYPALRLEIPEHDDNIDSSK
                     ELRIFIAIPLIFSSFSICLTCVARGSFTYQNNAMTFNDYEINIEKLIRATNLNELKKQ
                     CQAVSDKVNKKFEKDSMDFYYYNSFSQETP"
BASE COUNT          194 a           82 c           73 g          158 t
ORIGIN      
        1 taagtataaa tgaattaatt cgcaaacaaa aagatattga aaggatgcat accttaatta
       61 gtaaggttcc gggcggctat ccagctcttc gcttagagat tccagaacac gatgataaca
      121 ttgactcttc aaaggaatta agaatattta tcgccatccc tttaattttc tcatcttttt
      181 caatttgcct cacttgcgtt gccaggggct cctttacata tcaaaataac gctatgacat
      241 ttaatgatta tgaaatcaat attgaaaaac taattcgcgc aactaatctt aacgaactta
      301 aaaagcaatg ccaagcagtt tctgataaag taaataaaaa attcgagaag gatagtatgg
      361 atttttacta ttacaactca ttctcgcagg aaacccctta attaataagg aggaaattta
      421 agatgggaac aatataatta tttaataact tattgatttt taaatggttt tacaaaaaaa
      481 ataaaaatat gataacaacc gacttat
//
LOCUS       DAZFWL020000560          505 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZFWL020000560 DAZFWL020000000
VERSION     DAZFWL020000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.566"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>505
                     /locus_tag="ACHKNP_RS28460"
                     /old_locus_tag="pgaptmp_005692"
     CDS             <1..>505
                     /locus_tag="ACHKNP_RS28460"
                     /old_locus_tag="pgaptmp_005692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707597.1"
                     /translation="NNYLKQAWNNIKNEHEKNQKYSITLFENTLVCFMRLYKEIRRQK
                     AEDYIPCLECDSLEKEFEEMQNDNDLSLFLRTLRTNDTETYSGVSEGITYTIQYVRDI
                     DIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLDDENMSIQPEWIN
                     TPGISDLD"
BASE COUNT          183 a           62 c          101 g          159 t
ORIGIN      
        1 aaataattat ttgaaacaag cctggaacaa tataaaaaat gagcacgaaa aaaatcaaaa
       61 atattcaatc acgctttttg aaaacacact ggtgtgtttt atgcggttgt ataaagaaat
      121 cagacgtcaa aaagctgaag actacattcc atgcctggaa tgcgattcac tagaaaaaga
      181 atttgaggaa atgcagaatg ataatgattt atcattattt ttgagaacat tgcgtactaa
      241 tgatacagaa acttattcag gggtttcaga aggtattaca tatactatac aatatgttcg
      301 agatattgat attgttagag tgtccttgcc gggcagaggt tcagagtcta tcacagattt
      361 taaaggttat tattggtatg gctttatgga atatattgaa aacattaatg cgtgtgatga
      421 tgttttttct gagtattgtt tagatgatga aaatatgagt atccagccag aatggataaa
      481 tacgccggga atatctgatt tagat
//
LOCUS       DAZFWL020000561          505 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZFWL020000561 DAZFWL020000000
VERSION     DAZFWL020000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.567"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..388
                     /locus_tag="ACHKNP_RS28465"
                     /old_locus_tag="pgaptmp_005693"
     CDS             <1..388
                     /locus_tag="ACHKNP_RS28465"
                     /old_locus_tag="pgaptmp_005693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGU4707598.1"
                     /translation="DAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQ
                     ELKNARDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDI
                     IKAMNKAAALDELIPGLLSEYIEQSG"
     gene            363..>505
                     /locus_tag="ACHKNP_RS28470"
                     /old_locus_tag="pgaptmp_005694"
     CDS             363..>505
                     /locus_tag="ACHKNP_RS28470"
                     /old_locus_tag="pgaptmp_005694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4707599.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQ"
BASE COUNT          129 a          129 c          148 g           99 t
ORIGIN      
        1 ggatgctgaa attgagaacg aaaagctgcg ccgggaagtt gaagaactgc ggcaggccag
       61 cgagacagat ctccagccag ggactattga gtacgaacgc catcgactta cgcgtgcgca
      121 ggccgacgca caggagctga aaaatgccag agactccgct gaagtggtgg aaaccgcatt
      181 ctgtactttc gtgctgtcgc ggatcgcagg tgaaattgcc agtattctcg acgggatccc
      241 cctgtcggtg cagcggcgtt ttccggaact ggaaaaccga catgttgatt tcctgaaacg
      301 ggatatcatc aaagccatga acaaagcagc cgcgctggat gaactgatac cggggttgct
      361 gagtgaatat atcgaacagt caggttaaca ggctgcggca ttttgtccgc gccgggcttc
      421 gctcactgtt caggccggag ccacagaccg ccgttgaatg ggcggatgcc aattactatc
      481 tcccgaaaga atccgcatac cagga
//
LOCUS       DAZFWL020000562          505 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZFWL020000562 DAZFWL020000000
VERSION     DAZFWL020000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.568"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            101..>505
                     /locus_tag="ACHKNP_RS28475"
                     /old_locus_tag="pgaptmp_005695"
     CDS             101..>505
                     /locus_tag="ACHKNP_RS28475"
                     /old_locus_tag="pgaptmp_005695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HGU4707600.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTYPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIIS"
BASE COUNT          163 a           73 c           97 g          172 t
ORIGIN      
        1 ttataatttt tttattctgt gtcatcataa tttggtcatg aaatttccgt cttgttacac
       61 tactctaaac tagagcttat ggaattagcg ggtggtattc atggaaaata atgataaatt
      121 cttatcacaa gacttattgg aatcttatgc cattcgtttg ttgagcggac cattgaatgg
      181 atgcgagtat gaaatactta atgggcgtct acttgttatc attggtaatg atgtttcgtt
      241 aggaagaagt gatgcttttt ctgagttacc agaaaacaca atagtcgttc cttatggcga
      301 gcttacaggt agttttgaga taataattac tacctaccct gatctggtag taacattcag
      361 agaattaaca gcacaagaac ctgaagatag aacgttaaca ttgaatcagc aaatagaagt
      421 tttaggtctc aaatttgctg taaaagaaaa aaatgaagtt tggcagtatt cattgccagg
      481 cattattgaa aataacatta tttct
//
LOCUS       DAZFWL020000563          503 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZFWL020000563 DAZFWL020000000
VERSION     DAZFWL020000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.569"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            143..385
                     /gene="yiiF"
                     /locus_tag="ACHKNP_RS28480"
                     /old_locus_tag="pgaptmp_005696"
     CDS             143..385
                     /gene="yiiF"
                     /locus_tag="ACHKNP_RS28480"
                     /old_locus_tag="pgaptmp_005696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HGU4707601.1"
                     /translation="MNSLAGIDMGRILLNLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
BASE COUNT          157 a           90 c          119 g          137 t
ORIGIN      
        1 cttataataa ctgcaactgt tacatcgtat ctggaaaacg cctcgcaaaa ataaaaaatg
       61 atgcgtaaat gagctttctt taaagattcg gcaagggtac ttttctctcc atagattcaa
      121 aaatgatatt ggcgagatat ttatgaactc tctggcaggt atagatatgg gcagaatttt
      181 actcaattta tcgaatgagg tgattaagca acttgatgat ctcgaggtgc agcgtaatct
      241 tcctcgtgcg gacctattaa gggaagcggt agatcaatac ctgataaatc aatcgcaaac
      301 agcaagaacc agtgttcctg gcatctggca agggtgtgag gaagatggtg tcgaatatca
      361 gcgtaagctg cgcgaggaat ggtaatggga aaaaatgatg tcaatcagat tgctgacaac
      421 gtgcgcgttg ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aagtcatgca
      481 aattcaatat attgaagttt att
//
LOCUS       DAZFWL020000564          485 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZFWL020000564 DAZFWL020000000
VERSION     DAZFWL020000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.570"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>485
                     /locus_tag="ACHKNP_RS28485"
                     /old_locus_tag="pgaptmp_005697"
     rRNA            <1..>485
                     /locus_tag="ACHKNP_RS28485"
                     /old_locus_tag="pgaptmp_005697"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          110 c          163 g           91 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaacc
//
LOCUS       DAZFWL020000565          485 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZFWL020000565 DAZFWL020000000
VERSION     DAZFWL020000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.571"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>485
                     /locus_tag="ACHKNP_RS28490"
                     /old_locus_tag="pgaptmp_005698"
     CDS             <1..>485
                     /locus_tag="ACHKNP_RS28490"
                     /old_locus_tag="pgaptmp_005698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4707602.1"
                     /translation="LVGKSAFVEASGQASNDAEGYLDFFRGEIGKTHLAQGLWELIDN
                     SQLADEMAEMKTSITETRNEITQTVNKTLEDQSATIQQIQRVQKDTNDDLAALYMLKV
                     QKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPENGNSTPLFVAQGNQLFM
                     N"
BASE COUNT          143 a          107 c          149 g           86 t
ORIGIN      
        1 ctggtgggca agtctgcgtt tgtggaagcc agcgggcagg ccagcaatga tgcagaaggg
       61 tatctggact ttttcagagg agaaatagga aaaacgcatc tggctcaggg gttgtgggaa
      121 ctgatagata acagccagct tgcagatgag atggcggaga tgaagaccag catcacggaa
      181 acccgcaatg aaatcacaca gacggtcaac aaaacgctgg aggaccagag cgccaccata
      241 cagcagatac agcgcgtgca gaaggacaca aatgatgacc tggctgcgct gtacatgctg
      301 aaggttcaaa aaacgaaaga cggcattccc tatgtggccg ggattggtgc agggattgag
      361 gatactgatg gccagccact gagcaacata ctgctgctgg ctgaccgtat cgcgatgata
      421 aatccggaga acggcaacag cacgccgtta tttgtggcgc aggggaatca gctgttcatg
      481 aacga
//
LOCUS       DAZFWL020000566          476 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZFWL020000566 DAZFWL020000000
VERSION     DAZFWL020000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.572"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>476
                     /locus_tag="ACHKNP_RS28495"
                     /old_locus_tag="pgaptmp_005699"
     rRNA            <1..>476
                     /locus_tag="ACHKNP_RS28495"
                     /old_locus_tag="pgaptmp_005699"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZFWL020000567          467 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZFWL020000567 DAZFWL020000000
VERSION     DAZFWL020000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.573"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            106..>467
                     /locus_tag="ACHKNP_RS28500"
                     /old_locus_tag="pgaptmp_005700"
     CDS             106..>467
                     /locus_tag="ACHKNP_RS28500"
                     /old_locus_tag="pgaptmp_005700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HGU4707603.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVL"
BASE COUNT          133 a          117 c          105 g          112 t
ORIGIN      
        1 caaccaaatt ctccccacat caacctcatt gtgtgggtat aattgcgggc ataccccagt
       61 tcgacagaat ttgtaccctc tttaacgcat tcagaaggct gaactatggc actgactgac
      121 gcaaaaatcc gggctgcaaa gcccactgac aaggcttata aactcactga cggggctggc
      181 atgttcctgc tggtacatcc taatggttcc cgttactggc gtctccgtta tcgtattctg
      241 ggtaaagaga agactctggc acttggtgtg tatccagaag tatctctctc cgaagctcgt
      301 acaaaacggg atgaggcccg aaaactgatt tcggaggggg ttgacccttg cgaacagaaa
      361 agagctaaaa aagtagtccc tgatttacaa ctctctttcg aacatattgc acgacgctgg
      421 catgccagta ataaacaatg ggcacaatca cacagcgata aagtact
//
LOCUS       DAZFWL020000568          464 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZFWL020000568 DAZFWL020000000
VERSION     DAZFWL020000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.574"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>464
                     /gene="elaD"
                     /locus_tag="ACHKNP_RS28505"
                     /old_locus_tag="pgaptmp_005701"
     CDS             <1..>464
                     /gene="elaD"
                     /locus_tag="ACHKNP_RS28505"
                     /old_locus_tag="pgaptmp_005701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase ElaD"
                     /protein_id="HGU4707604.1"
                     /translation="SDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNEL
                     SDDTNFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENI
                     KSDPSWPNEVQLFHINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGH"
BASE COUNT          167 a           97 c           74 g          126 t
ORIGIN      
        1 tcagatgttc tcctgttcaa aaagctattc tccccttcga aattaatgac aattatcggt
       61 gatgaaattc ctcttatatc tgaaaaacag tcgctttcaa aggtgctttt aaatgatgag
      121 aataatgaac tgagtgatga tacaaacttc tgggataaaa atcgtcaatt aaccacagat
      181 gaaatagctt gctatcttca gaagatcgcc gctaatgcaa aaaatactca agtcaattat
      241 cctactggtc tctacgtccc ctactccacc agaactcacc tggaagacgc tctcaatgaa
      301 aatattaaga gcgatccatc atggccgaat gaagtccagt tattccacat aaatactggc
      361 ggacactgga tattagtttc gctacagaaa atagtaaata aaaaaaataa taaactacaa
      421 ataaaatgcg tcatattcaa ctcattgcgt gcactaggcc atga
//
LOCUS       DAZFWL020000569          462 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZFWL020000569 DAZFWL020000000
VERSION     DAZFWL020000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.575"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>462
                     /locus_tag="ACHKNP_RS28510"
                     /old_locus_tag="pgaptmp_005702"
     CDS             <1..>462
                     /locus_tag="ACHKNP_RS28510"
                     /old_locus_tag="pgaptmp_005702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4707605.1"
                     /translation="EQNRLIDEQASRVKSLQEKIAGYQYVLANPGWTTGDGFMINHLT
                     SVKTVTEGLSQATEQLAVEQSRLAQMQEKVQSIQDVLAGLEDRRVALIRQQAAEQNKV
                     YQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSAL"
BASE COUNT          117 a          111 c          143 g           91 t
ORIGIN      
        1 cagagcagaa ccggctgatt gatgaacagg ccagccgggt gaaatccctg caggaaaaaa
       61 tcgctggata tcagtatgtt ctggctaacc ctggctggac aaccggtgac ggattcatga
      121 taaaccatct gacatcggtg aagaccgtga cggaagggct ttctcaggca acagagcagc
      181 ttgccgttga gcagtcccgt ctggcacaga tgcaggaaaa agtgcagtcc attcaggatg
      241 tgcttgccgg gctggaagac cgtcgtgtgg cgttaattcg tcagcaggcg gcagagcaga
      301 ataaggtgta ccagtccatg ctggttatga acggtcagca tacggaattc aaccgtctgc
      361 tggggctggg gaatgaactg cttcagcagc gtcagggact ggtgaatgta ccgttacggc
      421 tgccgcaggc caccctggat gataaacagc agagcgccct ga
//
LOCUS       DAZFWL020000570          462 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZFWL020000570 DAZFWL020000000
VERSION     DAZFWL020000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.576"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..462
                     /locus_tag="ACHKNP_RS28515"
                     /old_locus_tag="pgaptmp_005703"
                     /pseudo
     CDS             1..462
                     /locus_tag="ACHKNP_RS28515"
                     /old_locus_tag="pgaptmp_005703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          113 a          101 c          152 g           96 t
ORIGIN      
        1 ccgttcgcgt cggccgggaa aacagtttca gcacaactgc ctgacggcgc atctgacggt
       61 aataaggcaa tcagtgaaac agagcatgat gcagtgaaag cgatgttcag ggggatactg
      121 agaaaatgac ggaacgggaa ctgtccatta ttcgtgcact gggcgaagaa ttctccacgg
      181 tgctggcgga tttacagcgc acatttgagg ggaagatggc cgcgcaggca caagcgtttg
      241 aagagaaact ggcttccctg tcggcggtat tacagaagca tgtgacggtg gatgaggtac
      301 atccggttct gcaggcgatg gtggatgacg ctgtggggac cattccggta ccgcgtgatg
      361 gtcgtgatta tgatccggat gtactgcagc aggcggtgaa tgatgcggtt gcgaatattc
      421 cggtaccggc ggacggcaaa agtatcaccc ccgatgatgt gc
//
LOCUS       DAZFWL020000571          461 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZFWL020000571 DAZFWL020000000
VERSION     DAZFWL020000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.577"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..245)
                     /locus_tag="ACHKNP_RS28520"
                     /old_locus_tag="pgaptmp_005704"
     CDS             complement(<1..245)
                     /locus_tag="ACHKNP_RS28520"
                     /old_locus_tag="pgaptmp_005704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGU4707606.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTG"
     gene            184..366
                     /locus_tag="ACHKNP_RS28525"
                     /old_locus_tag="pgaptmp_005705"
     CDS             184..366
                     /locus_tag="ACHKNP_RS28525"
                     /old_locus_tag="pgaptmp_005705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707607.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICFLQKVMIIDKTKYK"
BASE COUNT          138 a          111 c           90 g          122 t
ORIGIN      
        1 ccggttttac ccgtaccact ttcaacatcc acaccctcta ctgatctcag agcctccgcc
       61 agatcgtggt actggctgga ctgcaattcc tcttggctaa tcacagaaac actggcggct
      121 gcgttagtca gctttttctc atagccggat gccgagacaa tcatcacatc ctctgcagca
      181 gctatgctgc tggctgaaaa tccgagacag ggaatgacta cggaagccag agtggttatt
      241 cgcatattac taatctccga tcatgttaac cggcaagaca atacacaaat acaaacaata
      301 atgagaataa tttatatttg ctttttgcaa aaagtaatga ttattgacaa aactaaatat
      361 aaataacgac tgcctcggca tgccagttaa cagctatccg tatggcgtgt tcggttttgc
      421 gaataaaaaa tggttactgt agaagcggat ttccaccaca t
//
LOCUS       DAZFWL020000572          461 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZFWL020000572 DAZFWL020000000
VERSION     DAZFWL020000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.578"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            243..>461
                     /locus_tag="ACHKNP_RS28530"
                     /old_locus_tag="pgaptmp_005706"
     CDS             243..>461
                     /locus_tag="ACHKNP_RS28530"
                     /old_locus_tag="pgaptmp_005706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707608.1"
                     /translation="MKVRSIGFTINNNNKNINTVDVMNAFINASNREHSRTDYTRKIL
                     ISDVNDFYYGLVVTFRNQKKNCKSQFVDG"
BASE COUNT          160 a           89 c           82 g          130 t
ORIGIN      
        1 tgacatacca ataactcttg tctaaaaagc cagcaagata aaaagccagt attcacgacc
       61 accagcgtgt ttactgtact gcacaaggtt tacaggtaca aaaaaaccgc tcagcggcgg
      121 gtttaagttg tgtggcgaag taaccactct taacacgata taatactttt tgcgtacgcg
      181 ttaggatttt tataaactat gcgtccccat tctcgctatc tttagtcggt cctggaatac
      241 acatgaaagt tagaagcata ggatttacaa taaataacaa taacaaaaat attaataccg
      301 ttgacgtaat gaatgctttt atcaacgcat caaacagaga acacagtcgc acagactata
      361 ctcgtaaaat tctcatttcg gatgtgaatg atttctatta tggattagtt gtcacattca
      421 gaaaccaaaa aaagaactgt aagtcgcaat ttgttgatgg t
//
LOCUS       DAZFWL020000573          455 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZFWL020000573 DAZFWL020000000
VERSION     DAZFWL020000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.579"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(81..326)
                     /locus_tag="ACHKNP_RS28535"
                     /old_locus_tag="pgaptmp_005707"
     CDS             complement(81..326)
                     /locus_tag="ACHKNP_RS28535"
                     /old_locus_tag="pgaptmp_005707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4707609.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            complement(367..>455)
                     /locus_tag="ACHKNP_RS28540"
                     /old_locus_tag="pgaptmp_005708"
     CDS             complement(367..>455)
                     /locus_tag="ACHKNP_RS28540"
                     /old_locus_tag="pgaptmp_005708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4707610.1"
                     /translation="WRRQIDKRAAEKISAGQSAGSKPEEPLI"
BASE COUNT           90 a          142 c          112 g          111 t
ORIGIN      
        1 cataccccgc ctcccgcaat ccggaagctc acaacaataa aaaagaccac cggcacacac
       61 cgatggtccc tgacgcatgc ttacatcatc atgtcgctgt ccggtgtggg gtcaccgcta
      121 tctgaagcac tcccctcacc cgcgatgcct tccggctccg gagctgccgg tgcgcccagc
      181 agttcatcca gaatggcatc cacttctgca tcaagacgcg cttccaggtt atggcgaagt
      241 ttctgtttca gtgcgctccg gacttcttca gagcgcagga cttccttcac tgcttcagca
      301 gtgaccaggg atgtaatttc tgacatggga ttttctcgtc gaaaggtgtg attaagaaag
      361 ttgccgctaa atgagcggct cttcgggttt gcttccggct gactgaccgg cgctgatttt
      421 ctcagcggcc cttttgtcaa tctgcctgcg ccagc
//
LOCUS       DAZFWL020000574          453 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZFWL020000574 DAZFWL020000000
VERSION     DAZFWL020000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.580"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..396
                     /gene="espW"
                     /locus_tag="ACHKNP_RS28545"
                     /old_locus_tag="pgaptmp_005709"
     CDS             <1..396
                     /gene="espW"
                     /locus_tag="ACHKNP_RS28545"
                     /old_locus_tag="pgaptmp_005709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HGU4707611.1"
                     /translation="EYLYSGNVIKLSQLSIRYLPNINSISLIETKQSLLLHRLYSDEV
                     LQRNGTLIPTPLHEEKSIPADNIKTMLNNIPTYKMLPPFTETQGNCSSGAATFLRKSG
                     AEEKDILACSPRNYGLHHNIKTWDPLVRN"
BASE COUNT          172 a           91 c           69 g          121 t
ORIGIN      
        1 gagtatcttt attcaggaaa tgtaataaaa ctcagtcagt tatcaattcg ttacttaccc
       61 aatatcaact caatctcatt aatagagaca aaacagagtt tgttgctaca tcgattatat
      121 tcagatgaag tacttcagag aaatggaaca cttatcccga caccactaca tgaagaaaaa
      181 tcaattccag ctgacaatat aaaaacaatg ctcaacaaca taccgactta caaaatgtta
      241 ccgccattca cagaaacaca aggtaattgt tcttctggcg cagccacgtt tttacgcaaa
      301 tcaggcgccg aagaaaaaga tattcttgca tgtagccccc gaaattatgg gctgcatcat
      361 aacataaaaa catgggaccc cttggttaga aattaaggat ccaaatatta tatatttcat
      421 ataaagcaag gaaaatattg cctgaataat tgt
//
LOCUS       DAZFWL020000575          453 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZFWL020000575 DAZFWL020000000
VERSION     DAZFWL020000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.581"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>453
                     /locus_tag="ACHKNP_RS28550"
                     /old_locus_tag="pgaptmp_005710"
     CDS             <1..>453
                     /locus_tag="ACHKNP_RS28550"
                     /old_locus_tag="pgaptmp_005710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4707612.1"
                     /translation="VRYTAAVPARVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKI
                     IIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVF
                     RVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADP"
BASE COUNT          110 a          101 c          142 g          100 t
ORIGIN      
        1 gtgcgttaca cggcggcggt gcctgcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggctc cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtcct gatgaggaag agacgctgtt acgtgtggat
      181 gcggcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgactat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgca tctgtctatg gcaagccggt gatcaccatg
      361 ccaaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga caccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat ccc
//
LOCUS       DAZFWL020000576          453 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZFWL020000576 DAZFWL020000000
VERSION     DAZFWL020000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.582"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>453
                     /locus_tag="ACHKNP_RS28555"
                     /old_locus_tag="pgaptmp_005711"
     CDS             <1..>453
                     /locus_tag="ACHKNP_RS28555"
                     /old_locus_tag="pgaptmp_005711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4707613.1"
                     /translation="VRYTAAVPSRVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKI
                     IIMGRPDEEGTLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVF
                     RVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADP"
BASE COUNT          108 a          103 c          143 g           99 t
ORIGIN      
        1 gtgcgttaca cggctgcggt gccttcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggcac cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtccc gatgaggaag ggacgctgtt acgtgtggat
      181 gcggcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgaccat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgcg tctgtctatg gcaagccggt gatcaccatg
      361 ccgaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga caccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat ccc
//
LOCUS       DAZFWL020000577          444 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZFWL020000577 DAZFWL020000000
VERSION     DAZFWL020000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.583"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..297
                     /locus_tag="ACHKNP_RS28560"
                     /old_locus_tag="pgaptmp_005712"
     CDS             <1..297
                     /locus_tag="ACHKNP_RS28560"
                     /old_locus_tag="pgaptmp_005712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707614.1"
                     /translation="DSAGSLGLLLRSSNNKKEYNADKSKIVLTLDGEQYPFSNLGSMV
                     GDSWWYAFWQDAADSNANAIDAYVDNEKIATFPLSGLSKLYQQAKNDGCLKRGD"
BASE COUNT          140 a           67 c           99 g          138 t
ORIGIN      
        1 gatagtgccg gtagtttagg cctgttgcta aggtcgtcga ataataaaaa agagtataat
       61 gctgacaaga gtaaaatagt attaacatta gatggtgagc aatacccttt tagcaatctt
      121 gggtcaatgg ttggagattc ttggtggtat gccttttggc aagatgcagc agattcaaat
      181 gctaatgcga tcgatgctta tgttgataat gaaaaaattg ccacatttcc tctttctggg
      241 ctaagtaaat tatatcaaca agctaaaaac gatggatgct taaagcgcgg cgattaattt
      301 tattgcaaat ttaacgtaac ccataaagcg attatgggtt acaaaatatt aagatgttgt
      361 gttataacag gctgtttctt caaagcaatg ggtcaggtta ttgatcacag gttctccttt
      421 ctcgtgtctg agaataagag agcc
//
LOCUS       DAZFWL020000578          443 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZFWL020000578 DAZFWL020000000
VERSION     DAZFWL020000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.584"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          127 a           90 c          108 g          118 t
ORIGIN      
        1 atgtcatgat atcgccgtcg ttaacaggta attcttctat gactccagca gacaggcgca
       61 ggcaggaggc ggtcacctga ttactgaaca accttctgac tgacttcagc gtgtcaatca
      121 caggctccac gtcatgcgtc agcatagtgc tgatgctgac ggatgttaca aaaaatgcag
      181 cgtatggcgt tgagatagaa agtctggtgc tggagataaa tacacctaca tcatgataaa
      241 aaagggccag cgactgacct gaagaagaca ctggctaaaa ggctttgcat gtttgtagat
      301 acttattttt cacagacagc aatgatgcct gtcaatatat tattaatatg ctgattgttt
      361 cagttacaga tgctttatta agggaaaatg tcacgatcgg tgcaaaagtg gtttgaaccg
      421 ccccggtttt cctgaaggct ggg
//
LOCUS       DAZFWL020000579          439 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZFWL020000579 DAZFWL020000000
VERSION     DAZFWL020000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.586"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(84..380)
                     /locus_tag="ACHKNP_RS28565"
                     /old_locus_tag="pgaptmp_005713"
                     /pseudo
     CDS             complement(84..380)
                     /locus_tag="ACHKNP_RS28565"
                     /old_locus_tag="pgaptmp_005713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          101 a          116 c           89 g          133 t
ORIGIN      
        1 actttggttc ccactactta tttgttggac accactttgt ctaattcgtc agattctgac
       61 cagacggttc aggctgtacg cttacgatta cgctgaactt cacgtgagat cgtcgaagga
      121 ctgcgattca gcgcagtagc tatcgcacga atgctcattt tggctgacaa accagctcgt
      181 atctcctcgc gctcagacag tgtcaggtga gctacagccc gcttacgctc atggggtttt
      241 atgccgccag tatcccttaa catagtgaag atcgttccgg gttttgaacc caggatattc
      301 gctatttcac tgaagcctgt tccgttcttc catagttcaa aaacagaggc tttttcctct
      361 gctgtaaatg ttcgtctcat tcaaaaaacc tccgcaaccc catgttttca cataactgtt
      421 gcgttgacca attgaatct
//
LOCUS       DAZFWL020000580          435 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZFWL020000580 DAZFWL020000000
VERSION     DAZFWL020000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.587"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(15..197)
                     /locus_tag="ACHKNP_RS28570"
                     /old_locus_tag="pgaptmp_005714"
     CDS             complement(15..197)
                     /locus_tag="ACHKNP_RS28570"
                     /old_locus_tag="pgaptmp_005714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707615.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTEDN
                     DEAASDYALKINENSD"
BASE COUNT          141 a           95 c           67 g          132 t
ORIGIN      
        1 cagctacccc cgtattaatc agagttttca tttattttta atgcataatc agatgccgcc
       61 tcatcattat cctcagtatt atcatagccg aaaacttctc ccggacacgg gcgttcacga
      121 taatagtcat cgtcatagtt ttcatcgtca gataatgata tgtcgtattc tgaatatgat
      181 tcaccacaat tatacatgaa atcatctcct ttgggggggg caataattag tgagtttatt
      241 cgaaaaaatg atgagcgcca gaccaatcca cccaactttt gattataatc atgaagatac
      301 ttgtctcagt acaaattgaa tcaaactcaa tgcagcatga ttcaccttta tattctctca
      361 gggaaaccaa ataccactac tcaactatta agaacaaaat tcgggatgta tgattatcag
      421 aagatacctg catcc
//
LOCUS       DAZFWL020000581          432 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZFWL020000581 DAZFWL020000000
VERSION     DAZFWL020000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.588"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..400
                     /locus_tag="ACHKNP_RS28575"
                     /old_locus_tag="pgaptmp_005715"
     CDS             <1..400
                     /locus_tag="ACHKNP_RS28575"
                     /old_locus_tag="pgaptmp_005715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HGU4707616.1"
                     /translation="FRGRFNSKEITEHVFHTTKFSGDEKYIKFHCNSVEELKPSKLDV
                     IAKPGDILIARVGRNFHKKILFVESGYSYISDCIFLIRASGGDKKKLFDFLCSQDGQE
                     ELSRASSGVAAQHITMDALKKIHLVRIKHD"
BASE COUNT          148 a           54 c           91 g          139 t
ORIGIN      
        1 ttttagaggt aggtttaatt caaaggaaat tacggaacat gtttttcata cgacaaaatt
       61 tagtggtgat gaaaagtaca ttaaattcca ctgcaactct gtagaagaat tgaagccatc
      121 aaaattagat gtcattgcta agcctggtga tatattaata gcaagagttg ggcgaaattt
      181 tcataaaaaa atattgtttg ttgagagcgg ctattcttat atcagtgact gtatttttct
      241 gatacgagcc tctggtggag ataagaaaaa actatttgat tttctttgtt ctcaagatgg
      301 gcaagaggaa ttatctcgag cgagtagtgg tgtagccgca caacatatta caatggatgc
      361 attaaaaaaa atacatcttg taaggattaa acatgactga tgtggaatta gatagtaatt
      421 ttcttatcga gg
//
LOCUS       DAZFWL020000582          428 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZFWL020000582 DAZFWL020000000
VERSION     DAZFWL020000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.589"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..>428)
                     /locus_tag="ACHKNP_RS28580"
                     /old_locus_tag="pgaptmp_005716"
     CDS             complement(1..>428)
                     /locus_tag="ACHKNP_RS28580"
                     /old_locus_tag="pgaptmp_005716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4707617.1"
                     /translation="PQGPKGDRGERGETGLTGNAGPQGPKGDTGAAGPAGPQGPKGET
                     GAAGPVGATGPQGPKGDPGETQIRFRLGPASIIETNSNGWFPGTDGALITGLTFLDPK
                     DATQVQGLFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT           64 a          161 c          101 g          102 t
ORIGIN      
        1 tcattctcct gttctgcctg tatcactgcc cacttcatca agccccttaa catcctgcca
       61 cggcccgtca ccaaacctga cctgcaaatg ctgaaacagc ccctgaacct gtgtggcatc
      121 tttggggtca agaaaggtca gtccggtgat gagcgcacca tctgtacccg ggaaccagcc
      181 attgctgttt gtctcaataa tgctcgccgg ccccagacga aaacggattt gtgtctcccc
      241 cgggtcgccc tttggtccct gaggtccggt tgcccccacc gggccagccg cacctgtttc
      301 tcctttcggt ccctgtgggc cagccgggcc tgccgcaccg gtatctccct ttggcccctg
      361 tggacctgca tttcccgtca gaccggtctc tccccgctct cccctgtcgc ctttcggccc
      421 ctgcggac
//
LOCUS       DAZFWL020000583          424 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZFWL020000583 DAZFWL020000000
VERSION     DAZFWL020000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.590"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..217
                     /locus_tag="ACHKNP_RS28585"
                     /old_locus_tag="pgaptmp_005717"
     CDS             <1..217
                     /locus_tag="ACHKNP_RS28585"
                     /old_locus_tag="pgaptmp_005717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HGU4707618.1"
                     /translation="KFSRNKHLIETSYNKLKSKEITFQQYRENLAKAGVFRWVTNIQE
                     HQRYYYAFDNSLLFTESIQKTTQILPR"
     gene            complement(369..>424)
                     /locus_tag="ACHKNP_RS28590"
                     /old_locus_tag="pgaptmp_005718"
     CDS             complement(369..>424)
                     /locus_tag="ACHKNP_RS28590"
                     /old_locus_tag="pgaptmp_005718"
                     /inference="COORDINATES: protein motif:HMM:NF019786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGU4707619.1"
                     /translation="REKMTGLESYDVKINLI"
BASE COUNT          158 a           89 c           57 g          120 t
ORIGIN      
        1 taaattctcc agaaacaaac atcttataga gacctcctat aataagttga aatcaaaaga
       61 aatcacattt caacaataca gggaaaatct tgctaaagca ggagttttcc gatgggttac
      121 aaatatccag gaacaccaaa gatattacta tgcctttgat aactcattac tatttactga
      181 aagcatccag aaaactacac agatcttacc acgctaaacc ataacgtccg gcttctctca
      241 ctcctgagcc ggactgcatt ggtttaataa aaaccatcaa caattgtgat ttagatattc
      301 ggaaccattc aaatataaca aaaccccgaa aaaacgaggt ttatggataa attttattat
      361 tgaatacatc agattaaatt aatcttgaca tcatagcttt caagacccgt cattttttcc
      421 cgtg
//
LOCUS       DAZFWL020000584          424 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZFWL020000584 DAZFWL020000000
VERSION     DAZFWL020000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.591"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            38..364
                     /locus_tag="ACHKNP_RS28595"
                     /old_locus_tag="pgaptmp_005719"
     CDS             38..364
                     /locus_tag="ACHKNP_RS28595"
                     /old_locus_tag="pgaptmp_005719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4707620.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
BASE COUNT           96 a           92 c          145 g           91 t
ORIGIN      
        1 tggctgcaaa acgtggacgg gatgcaggag gcggaaaatg acggcattac tgacactgga
       61 agagatcaag gcacatctgc gtgttgacca tgacgcggat gatgacatgc tgatggacaa
      121 ggttcgtcag gctaccgccg tgctgctggc ctacattcag ggcagccggg ataaagtgat
      181 tcgtgaggac ggtgagctga tcccgggcga ggcattaacc cggatgaagg gggctgccat
      241 gcgactgacc gggatgctgt accggaatcc ggatcttgct gagcgggaag acctcgtgca
      301 gggggaactg ccgttttctg tgtccgtgct gatttacgat ttgcgttgtc cgacggtgtt
      361 atgaggaggg aggaatggca atatctgcag gtcgtctgac acagatgata agtgttctga
      421 accc
//
LOCUS       DAZFWL020000585          419 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZFWL020000585 DAZFWL020000000
VERSION     DAZFWL020000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.592"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          117 a           90 c           94 g          118 t
ORIGIN      
        1 ggtgttcaga tgtcgtttca tcagcttttc cttacagagg gtgaataaaa aagccggtac
       61 ccacaaatgt ggataccggc aggtacaaaa cagcatgctc agataaagtg gtttttctcg
      121 gacataaatg cttcccctct ctcatccgcc agagaagtat cagtccgttg tgtgcaattc
      181 cgttctgcat gatgaagaga ttcagctgcc ttttcgcaga ccattaacca ctataaacga
      241 tcgatagcaa cgatcgatac ctgttttatc gatcgtttta ttctattaac gacaaggcgg
      301 tcacgcaaga gaaacaggag gagtatgagg ggtttgtgtc atgttttcag ttggttaacg
      361 taatgttatc aggcatttcc ctggcagact gttcagaaga aaatccagtt ccatacagc
//
LOCUS       DAZFWL020000586          418 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZFWL020000586 DAZFWL020000000
VERSION     DAZFWL020000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.593"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            141..>418
                     /locus_tag="ACHKNP_RS28600"
                     /old_locus_tag="pgaptmp_005720"
     CDS             141..>418
                     /locus_tag="ACHKNP_RS28600"
                     /old_locus_tag="pgaptmp_005720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HGU4707621.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALRE"
BASE COUNT          104 a          100 c          115 g           99 t
ORIGIN      
        1 ccttttttgt atctgcgcca cgcccggcgc atatcaacca cagagccttt cgggggtgag
       61 cttacggagt ggtcagtgtg actttctctg tgggcagatc gctcccgggc gttggctcac
      121 ccacccaaag gaacgtcacg atgtttggaa tcttcaaaaa gaaaacccgc agagcggcag
      181 cggaaattaa aaagtttgag aaacgcgatc tggcacaggc ggtgattaac gctgcatacc
      241 tggtggccta tgcagatggt gaatgcgagg catccgagaa agcgaagatc gaacaggtct
      301 tacgtaatca gcctgcgttg tctgcgttta cctcggaaat taatgcgatt agcgcaacta
      361 ttatcggtca gctggatacc aattttaaaa ttggtcgtcg tgccgcgtta cgcgagat
//
LOCUS       DAZFWL020000587          416 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZFWL020000587 DAZFWL020000000
VERSION     DAZFWL020000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.594"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>416
                     /locus_tag="ACHKNP_RS28605"
                     /old_locus_tag="pgaptmp_005721"
     CDS             <1..>416
                     /locus_tag="ACHKNP_RS28605"
                     /old_locus_tag="pgaptmp_005721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HGU4707622.1"
                     /translation="ELRINYLKNVIKLSDDDIGNYQKTINDNKDRVKKLILDLQKQFG
                     ENRISIKDVNSLTSLSKSENNHNYQTEMLLRWNYPAASDLLRMYILKEHGGIYTDTDM
                     MPAYSKQVIFKIMMQTNGDNRFLEDLKLRRAISDGV"
BASE COUNT          159 a           68 c           67 g          122 t
ORIGIN      
        1 tgagttacga ataaactacc ttaaaaatgt aataaaactg tcagacgatg acattggtaa
       61 ttatcagaaa acaattaacg acaataaaga tagagtaaaa aaactaattc ttgatttaca
      121 gaaacaattt ggtgaaaacc gtatttcaat taaagatgtt aactctttaa cctctctttc
      181 aaaatcagaa aataatcaca attatcaaac tgaaatgttg cttcgatgga actatcctgc
      241 cgcctcagac ctgctcagga tgtatatcct taaagagcat ggtggtattt atacagacac
      301 agacatgatg cctgcatact ctaaacaagt aatttttaaa attatgatgc agacaaacgg
      361 agataatcgt ttcttggaag atttgaaact acgtcgtgca atatctgatg gtgtat
//
LOCUS       DAZFWL020000588          410 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZFWL020000588 DAZFWL020000000
VERSION     DAZFWL020000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.595"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..293)
                     /locus_tag="ACHKNP_RS28610"
                     /old_locus_tag="pgaptmp_005722"
     CDS             complement(<1..293)
                     /locus_tag="ACHKNP_RS28610"
                     /old_locus_tag="pgaptmp_005722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGU4707623.1"
                     /translation="MAFLLNKSDMASSIGISVQAFDKWGVPPVERRGREVFYDVKTVL
                     EIDRERRQHNQRIPDDEGDLEERLLRARAELTEEQAVAQKLKNQVTEGKLIDTG"
BASE COUNT          109 a          109 c           71 g          121 t
ORIGIN      
        1 ccggtgtcaa taagcttacc ttcggttacc tgatttttaa gtttttgagc tacggcctgt
       61 tcttctgtca gttcagctct ggcccgaagc agcctttcct ccagatcgcc ctcgtcatca
      121 ggtattctct gattgtgttg tcgccgctcg cgatctatct ccagtacagt tttaacgtca
      181 taaaaaactt ccctcccccg acgctcaaca ggaggaacgc cccatttatc aaatgcctga
      241 acagatatac cgatggagga ggccatatca cttttattca ataaaaaggc catctcctct
      301 ccataagcca tcgataaaaa gcgatacaac aaccatgtgt ttttacaaaa ccatttgata
      361 tcattgacat ttttcgcatt gacgacatca aaacacatcg taaggttgtt
//
LOCUS       DAZFWL020000589          409 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZFWL020000589 DAZFWL020000000
VERSION     DAZFWL020000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.596"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>409
                     /locus_tag="ACHKNP_RS28615"
                     /old_locus_tag="pgaptmp_005723"
     CDS             <1..>409
                     /locus_tag="ACHKNP_RS28615"
                     /old_locus_tag="pgaptmp_005723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4707624.1"
                     /translation="RPDQLWVADFTYVSTWQGFVCVAFIIDVFAGCIVGWRVSSSMET
                     TFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTERLKEAKLLASTGSTGDSYDNAM
                     AESINGLYKAEVIHRKSWKNRAEVELATLTWVDW"
BASE COUNT           97 a           91 c          127 g           94 t
ORIGIN      
        1 cgtcctgacc agctgtgggt ggctgatttt acttacgtca gcacatggca gggcttcgtc
       61 tgtgtggcgt tcatcattga tgtgtttgcc ggatgtatcg tggggtggcg agtctcatcg
      121 tctatggaaa cgacattcgt gctggatgca ctggagcagg cgttgtgggc ccgtcggccg
      181 tccggcacag tccatcacag tgataaaggt tctcagtatg tatcactggc ctatacggag
      241 cgactaaaag aagcaaaact gctggcatca acagggagta caggtgactc gtatgacaac
      301 gcgatggcgg agagcatcaa tggtctttac aaagcggagg taatacaccg taagagctgg
      361 aaaaaccgtg cagaagtgga actggccaca ctcacgtggg tggactggt
//
LOCUS       DAZFWL020000590          399 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZFWL020000590 DAZFWL020000000
VERSION     DAZFWL020000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.597"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>399
                     /locus_tag="ACHKNP_RS28620"
                     /old_locus_tag="pgaptmp_005724"
     CDS             <1..>399
                     /locus_tag="ACHKNP_RS28620"
                     /old_locus_tag="pgaptmp_005724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4707625.1"
                     /translation="TGLKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRI
                     VRALAAGMGVARKDLKAMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKV
                     ENAFMAWVGGANEASGVTKTLSGMLNGVAGQ"
BASE COUNT           93 a           86 c          141 g           79 t
ORIGIN      
        1 acagggctga aaatctccgg tgccagtacg gcagaggcgg gctcggtgat cacccagttc
       61 agtcaggcgc tggcgcaggg tgtattgcgt ggtgaggagt ttaattcggt caatgaaagt
      121 ggtgaccgga tcgtacgtgc actggctgcg ggtatgggcg tggcccgtaa agatctgaag
      181 gcaatggcgg atgacggaaa actgacagcg gataaagtgg tccccgcgtt aatcagccag
      241 ctggggatat tacgtgatga atatgcggcc atgccggaaa cggtttccag tagtatcaca
      301 aaggtggaaa acgcctttat ggcctgggtg ggcggtgcga atgaggccag cggggtgaca
      361 aaaacgctct ccggcatgct gaacggtgtt gccggacag
//
LOCUS       DAZFWL020000591          397 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZFWL020000591 DAZFWL020000000
VERSION     DAZFWL020000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.598"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(59..265)
                     /locus_tag="ACHKNP_RS28625"
                     /old_locus_tag="pgaptmp_005725"
     CDS             complement(59..265)
                     /locus_tag="ACHKNP_RS28625"
                     /old_locus_tag="pgaptmp_005725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HGU4707626.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          108 a           96 c           81 g          112 t
ORIGIN      
        1 atacaaaaaa ccgccagcgt ttccgctggc ggttttcaat gtattcaaaa ctcagcaatc
       61 aataaaaatc accagttgcc ttttcggcct gatccatcca caccggttta tcgctggttt
      121 tcgaccagac gcgatgcagg tagctgtaaa agcgggcgcg gtctttccag aaaagcatga
      181 aaggcagcgc cagaacgccc gctgcaaccg cataagtacg gcgtaaaaag ataagccatg
      241 caggatattc tctgtagagt tccatacttt tctccccctt ttgtatctac ccggtgaatg
      301 gtaccggaaa aatgaatttg tttatctgat gaaaatagta ccgccttttg tgtaatttta
      361 ctactcatcc gaccacttat ttttgcttat tgatggt
//
LOCUS       DAZFWL020000592          394 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZFWL020000592 DAZFWL020000000
VERSION     DAZFWL020000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.599"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          152 a           80 c           65 g           97 t
ORIGIN      
        1 aacgccagca acacatagtg ccggcggagg gaatacccca tggagaaaat gtgatgccta
       61 gagcatcatg aatatataat taaaaatagt tagcgttgtc aatactttca caaaaataat
      121 tttcgtagta tagaaagata tttttatgca tgacctacct gaatttgctc cgggtagagg
      181 ttataaataa aaattgaatc acgacaaaca caatattcat agtatggcga tgcctacgcc
      241 agcaagaata gcgccaataa tattgggaat ataatagaca ccagacgcac aggcatctca
      301 ctccttaaca aacaacaatc aggatattta cttttaccaa gctaactgtt tacacccaaa
      361 gtacacacaa ttaaccattc aataacaaat ggca
//
LOCUS       DAZFWL020000593          386 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZFWL020000593 DAZFWL020000000
VERSION     DAZFWL020000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.600"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>386
                     /locus_tag="ACHKNP_RS28630"
                     /old_locus_tag="pgaptmp_005726"
     CDS             <1..>386
                     /locus_tag="ACHKNP_RS28630"
                     /old_locus_tag="pgaptmp_005726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU4707627.1"
                     /translation="DNLKSTQLLSVIDAISEGPIEGPVDGLKSVLLNSTPVLDSEGNT
                     NISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITRTITSANIDRLRFTFG
                     VQALVETTSKGDRNPSEVRLLVQIQR"
BASE COUNT           92 a          100 c          122 g           72 t
ORIGIN      
        1 gataacctga aatccacgca gctgctgagt gtgattgatg ccatcagcga agggccgatt
       61 gaaggtccgg tggatggatt aaaaagcgtg ctgctgaaca gtacgccggt gctggacagt
      121 gaggggaata ccaacatctc cggcgtcacg gtggtgttcc gggcaggtga gcaggagcag
      181 acaccgccgg aaggttttga atcctccggc tccgagacgg tgctgggtac ggaagtgaaa
      241 tatgacacgc cgatcacccg caccatcacg tcggcaaaca tcgaccgtct gcgctttacc
      301 ttcggtgtgc aggcactggt ggaaaccacc tcaaaggggg accggaatcc gtcggaagtc
      361 cgcctgctgg ttcagataca gcgtaa
//
LOCUS       DAZFWL020000594          382 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZFWL020000594 DAZFWL020000000
VERSION     DAZFWL020000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.601"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>382
                     /gene="grlA"
                     /locus_tag="ACHKNP_RS28635"
                     /old_locus_tag="pgaptmp_005727"
     CDS             <1..>382
                     /gene="grlA"
                     /locus_tag="ACHKNP_RS28635"
                     /old_locus_tag="pgaptmp_005727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HGU4707628.1"
                     /translation="KNKNGDYVIPDSVKNYDGEPLYILVSLWCKLQEKWISRNDIAEA
                     FGINLRRASFIITYISRRKEKISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIESPVS
                     TGPKRKNYRVGNGIVGQSNIWNEMI"
BASE COUNT          126 a           58 c           82 g          116 t
ORIGIN      
        1 taaaaataaa aatggcgact atgtaattcc tgactcagta aagaattacg atggtgagcc
       61 tctgtatatc ttggtttctc tttggtgtaa attgcaggag aaatggattt ctcgcaatga
      121 tattgccgaa gcattcggta taaacctgag gagagcatca tttattataa cttatatatc
      181 gagaagaaaa gaaaaaattt catttcgtgt cagatatgtt agttatggta acttgcatta
      241 taagcgcctt gagattttca tttatgatgt taacctcgag gctgttccga tagaaagtcc
      301 ggtatcaacc ggaccaaaaa gaaaaaacta ccgagtcggt aatggtattg tggggcagtc
      361 taatatctgg aacgaaatga tc
//
LOCUS       DAZFWL020000595          380 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZFWL020000595 DAZFWL020000000
VERSION     DAZFWL020000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.602"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>380)
                     /locus_tag="ACHKNP_RS28640"
                     /old_locus_tag="pgaptmp_005728"
     CDS             complement(<1..>380)
                     /locus_tag="ACHKNP_RS28640"
                     /old_locus_tag="pgaptmp_005728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707629.1"
                     /translation="SVSASLYVFSAFSVFVFLGSIFVILELEFENNNKLILIRSMIIA
                     CIPIIYLFASSCASSLFLSLSNLNITLTPWVEYFWKGMAFMLLFFMLMQLVIYFAFLT
                     LWAKLSVYRLFILAGAFIVSTILV"
BASE COUNT          168 a           69 c           50 g           93 t
ORIGIN      
        1 cactaaaata gtagatacga taaatgctcc ggcaagtata aacaacctat atacacttaa
       61 tttagcccac agagttaaaa aagcaaaata gataacaagt tgcattaaca taaagaaaag
      121 caacataaaa gccatccctt tccagaaata ctcaacccat ggcgttagtg tgatattgag
      181 attactcaaa cttaaaaaca aactggaagc acaactactt gcaaacaaat atataattgg
      241 tatgcaagct ataatcatgg acctaataag tattagctta ttattgtttt caaattccaa
      301 ttctaatata acaaaaatac ttcccagaaa aacgaatact gaaaaagcac taaaaacgta
      361 tagagaggca ctgactgaaa
//
LOCUS       DAZFWL020000596          379 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZFWL020000596 DAZFWL020000000
VERSION     DAZFWL020000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.603"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>379
                     /locus_tag="ACHKNP_RS28645"
                     /old_locus_tag="pgaptmp_005729"
     CDS             <1..>379
                     /locus_tag="ACHKNP_RS28645"
                     /old_locus_tag="pgaptmp_005729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4707630.1"
                     /translation="RKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLD
                     HIGIDPSGVGQILDSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPL
                     MAWCVGNARVEPKGNAILITKQA"
BASE COUNT           80 a           87 c          136 g           76 t
ORIGIN      
        1 gacggaagag cgaggcgtcc cggtttcagg atcttgttgc ctgtggagat atgaccattg
       61 tccggcgtgt cggggatgac acggcggaag tggcggaata tgtgcgtcgc attcatgagg
      121 ctgagttact ggaccatatc ggtattgacc cgtcaggggt ggggcagatt ctggattcac
      181 tggcggaagc cgggatcccc gacggaattg tggtggggat aagccagggc tggaaactgg
      241 gcggggccat taaaaccacc gagcgcaaac tggctgaagg ggtgctggtg catggtgacc
      301 agcccctgat ggcctggtgt gtcggcaatg cccgggtgga gcctaaaggt aacgccattc
      361 ttatcaccaa acaggccag
//
LOCUS       DAZFWL020000597          378 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZFWL020000597 DAZFWL020000000
VERSION     DAZFWL020000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.604"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..357)
                     /gene="ler"
                     /locus_tag="ACHKNP_RS28650"
                     /old_locus_tag="pgaptmp_005730"
     CDS             complement(<1..357)
                     /gene="ler"
                     /locus_tag="ACHKNP_RS28650"
                     /old_locus_tag="pgaptmp_005730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HGU4707631.1"
                     /translation="MNMETNSHTTSPYIQLIEQIEVLQQEAKRLREQEIQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEDEIIP"
BASE COUNT           87 a           91 c           68 g          132 t
ORIGIN      
        1 cggtattatt tcgtcttcgg tgtccttcac aagaaaatct tctttcttca ttccattcaa
       61 cagtgcttct ttaagccagc gtggctgtcg gccaactccc gaccaggtct gcccttcttc
      121 attgcgataa taaactcctt tcgatgagtt ccggcgagca aggccatcat caggcacatt
      181 agtatatccc agctcttgta gggttatatt gtaataagta atctgctttt gaatcgactc
      241 aattacactt tgaatttcct gctctcgcag tcgctttgct tcctgttgta acacttcaat
      301 ttgctcaata agctgaatgt atgggcttgt tgtgtgcgaa ttagtttcca tattcataat
      361 aaataatctc cgcatgct
//
LOCUS       DAZFWL020000598          375 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZFWL020000598 DAZFWL020000000
VERSION     DAZFWL020000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.605"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          105 a           67 c           94 g          109 t
ORIGIN      
        1 ggtattcaga tgtcgtttca tcagcttttc cttagattga gggtgaataa aaaagccggt
       61 acccacaaac gtggataccg gcaggtaaag ggttacagtg ccggaggtcc ggctgtggga
      121 gtttctgaat tgtggttctg ttgttccctg aatgtctttc tgtacgtatc tgtgcatgtt
      181 actgacaaag gttattatta gcttatcgat cgatataaac gatcgataaa atagatattg
      241 atcgttttat tctattattg agctattgca cggacaagag agacagtaac gaaaacagtg
      301 cttttgtgag cagactcaga gtcagggagg gcatcagtca cgacaggttc agaagaaaat
      361 ccagttccat accgc
//
LOCUS       DAZFWL020000599          372 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZFWL020000599 DAZFWL020000000
VERSION     DAZFWL020000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.606"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            65..325
                     /gene="iraP"
                     /locus_tag="ACHKNP_RS28655"
                     /old_locus_tag="pgaptmp_005731"
     CDS             65..325
                     /gene="iraP"
                     /locus_tag="ACHKNP_RS28655"
                     /old_locus_tag="pgaptmp_005731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HGU4707632.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          119 a           71 c           90 g           92 t
ORIGIN      
        1 agacactatg aagttgtgaa acataatgtt aacttctcca tactttggat aaggaaatac
       61 agacatgaaa aatctcattg ctgagttgtt atttaagctt gcccaaaaag aagaagagtc
      121 gaaagaactg tgtgcgcagg tagaagcttt ggagattatc gtcactgcaa tgcttcgcaa
      181 tatggcgcaa aatgaccaac agcgcttgat tgatcaggta gagggggcgc tgtacgaggt
      241 aaagcccgat gccagcattc ctgacgacga tacggagctg ctgcgcgatt acgtaaagaa
      301 gttattgaag catcctcgtc agtaaaaagt taatcttttc aacagctgtc ataaagttgt
      361 cacggccgag ac
//
LOCUS       DAZFWL020000600          368 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZFWL020000600 DAZFWL020000000
VERSION     DAZFWL020000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.607"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(93..312)
                     /locus_tag="ACHKNP_RS28660"
                     /old_locus_tag="pgaptmp_005732"
                     /pseudo
     CDS             complement(93..312)
                     /locus_tag="ACHKNP_RS28660"
                     /old_locus_tag="pgaptmp_005732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT           90 a           75 c           93 g          110 t
ORIGIN      
        1 tcatgtgtta tgtatgtatt cagagtgccc ataattcacc agtgcaggga taaattgtaa
       61 aaaaatcgca gtacgtcgta tgcagaacgg tgcggcgtac gcagttccag tggaccgtcg
      121 cctgtgataa cggtctttgt ggaataaccg ttgcgggagt tagctcctga tctggactga
      181 tttttctcat tcccaagatg gtgtgtcatc tctgcattga gagcggcttc aacgctgagc
      241 tttttcagca accgatcaaa ctgactgagg tcttctgagg gggctgaggt ttttggccag
      301 ttcgttagca atacaccaga aaaattctaa acgagcctgt acatagattt gtgtaattgc
      361 ctgatttt
//
LOCUS       DAZFWL020000601          368 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZFWL020000601 DAZFWL020000000
VERSION     DAZFWL020000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.608"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>368
                     /locus_tag="ACHKNP_RS28665"
                     /old_locus_tag="pgaptmp_005733"
     CDS             <1..>368
                     /locus_tag="ACHKNP_RS28665"
                     /old_locus_tag="pgaptmp_005733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HGU4707633.1"
                     /translation="SPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSSENSGESS
                     DQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIR
                     LMRERDGRNHRDMCVLFRWA"
BASE COUNT          114 a           87 c           94 g           73 t
ORIGIN      
        1 tcccctaaaa cgagggataa aacatccctc aaattagggg attgctatcc ctcaaaacag
       61 ggggacacaa aagacactat tacaaaagaa aaaagaaaag attattcgtc cgagaattct
      121 ggcgaatcct ctgaccagcc agaaaacgat ctttctgtgg ttaaaccgga tgctgcaatt
      181 cagagcggca gcaagtgggg aacagcagaa gacctgaccg ccgcagagtg gatgtttgac
      241 atggtgaaga ccatcgcacc atcagccaga aaaccgaatt ttgcagggtg ggctaacgat
      301 atccgcctga tgcgtgaacg tgacggacgt aaccaccgcg atatgtgtgt gcttttccgc
      361 tgggcctg
//
LOCUS       DAZFWL020000602          360 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZFWL020000602 DAZFWL020000000
VERSION     DAZFWL020000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.609"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            169..>360
                     /locus_tag="ACHKNP_RS28670"
                     /old_locus_tag="pgaptmp_005734"
     CDS             169..>360
                     /locus_tag="ACHKNP_RS28670"
                     /old_locus_tag="pgaptmp_005734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4707634.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGT"
BASE COUNT           90 a           89 c           89 g           92 t
ORIGIN      
        1 aaaatcggcc agttttctct gcctgcagtc cgcatgccgt atcgggcctt gggttctaac
       61 ctgttgcgta gatttatgca gcggactgcc tttctcccaa agtgataaac cggacagtat
      121 catggaccgg ttttcccggt aatccgtatt tgcaaggttg gtttcactat ggaacatgaa
      181 cttcattata tcggtatcga caccgctaaa gagaaactgg atgtcgatgt gttgcgtcct
      241 gatggtcgtc atcgcaccaa aaaattcgct aacaccacta aagggcacga tgagctggtg
      301 agctggctga aaggtcacaa gattgaccat gcgcatatct gcatcgaagc gaccggcacc
//
LOCUS       DAZFWL020000603          356 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZFWL020000603 DAZFWL020000000
VERSION     DAZFWL020000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.610"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..211)
                     /locus_tag="ACHKNP_RS28675"
                     /old_locus_tag="pgaptmp_005735"
     CDS             complement(<1..211)
                     /locus_tag="ACHKNP_RS28675"
                     /old_locus_tag="pgaptmp_005735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4707635.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKE"
     gene            complement(201..356)
                     /locus_tag="ACHKNP_RS28680"
                     /old_locus_tag="pgaptmp_005736"
                     /pseudo
     CDS             complement(201..356)
                     /locus_tag="ACHKNP_RS28680"
                     /old_locus_tag="pgaptmp_005736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
BASE COUNT          118 a           80 c           53 g          105 t
ORIGIN      
        1 tttccttccc aaccggcatc attacgaaca ttgacaatag caaagcaacc ccattaagtg
       61 tcatatttga tggcacttgc tgaagtccca gcgcgtttcg gacaataaca aaaacaatcg
      121 agaatttaat aaaacatgtc ccggatgcta ttataaacgg caatagtgta aataatgata
      181 atatcgctat cagcgaaatg ctatttgaca ttattattcc ctgataacca agaatgaatt
      241 tctacgccca atttatcatc cacttcaaca agttcgccat agccagttag cgcaccattt
      301 acacgtatct ctatattttc tcagattcag gaagcagaga tattattctt tttgca
//
LOCUS       DAZFWL020000604          350 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZFWL020000604 DAZFWL020000000
VERSION     DAZFWL020000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.611"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>350
                     /locus_tag="ACHKNP_RS28685"
                     /old_locus_tag="pgaptmp_005737"
     CDS             <1..>350
                     /locus_tag="ACHKNP_RS28685"
                     /old_locus_tag="pgaptmp_005737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707636.1"
                     /translation="TLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVET
                     YGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLN
                     SVIPPAVQHLTVEV"
BASE COUNT           71 a          108 c          111 g           60 t
ORIGIN      
        1 accctgccgg agacaggtac atcggcggtg aacctgatta acggcagcgg taagccggtg
       61 agtgtggaca tcaccgcaca ccccgcgccg gaccggatac aggtcagtac cctgcctgat
      121 ggtgtggaga catacggggt gtggggactc tccctgccgt cactgcgccg tcgcctgttc
      181 cgctgtgtct ccgtccggga aaacacggac ggcacctttg ccatcacggc ggtgcagcac
      241 gtaccggaaa aagaagccat cgtggataac ggtgcccgct ttgagccgca gtcaggttcc
      301 ctgaacagcg tcatcccacc ggcagtacag cacctgacgg tggaggtgag
//
LOCUS       DAZFWL020000605          349 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZFWL020000605 DAZFWL020000000
VERSION     DAZFWL020000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.612"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..245)
                     /locus_tag="ACHKNP_RS28690"
                     /old_locus_tag="pgaptmp_005738"
     CDS             complement(<1..245)
                     /locus_tag="ACHKNP_RS28690"
                     /old_locus_tag="pgaptmp_005738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4707637.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVS"
     gene            complement(245..>349)
                     /locus_tag="ACHKNP_RS28695"
                     /old_locus_tag="pgaptmp_005739"
     CDS             complement(245..>349)
                     /locus_tag="ACHKNP_RS28695"
                     /old_locus_tag="pgaptmp_005739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707638.1"
                     /translation="PYAWRQIKVTCAAWSSRVRMLRVEFSAEFKQVVN"
BASE COUNT           76 a          122 c           71 g           80 t
ORIGIN      
        1 gacaccgtca gcgacggtct ggcactgctg cccttcccgt tcatctcaaa accgctgccg
       61 tcaatcgggt atacctgata ttgccgcccc tgccaggtga ccgcctcccc tttttcattc
      121 agctcattac agaaaaaata ccgctcaccg ccctgcaccg tcaggtcaat ttcccagagc
      181 accacccgcg gtgactgctc tgatttaacc gactcgttca gactttcttc gtgaatatcc
      241 tgcatcagtt caccacctgc ttaaactcgg cactgaattc aacccgcagc atgcgaaccc
      301 gtgatgacca ggcggcacag gtcaccttta tctgccgcca ggcataagg
//
LOCUS       DAZFWL020000606          348 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZFWL020000606 DAZFWL020000000
VERSION     DAZFWL020000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.613"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>348
                     /locus_tag="ACHKNP_RS28700"
                     /old_locus_tag="pgaptmp_005740"
     CDS             <1..>348
                     /locus_tag="ACHKNP_RS28700"
                     /old_locus_tag="pgaptmp_005740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4707639.1"
                     /translation="YRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARSAAQNAL
                     NSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALTIDEIAH
                     KTPSMSLPEASDNE"
BASE COUNT           79 a           92 c          118 g           59 t
ORIGIN      
        1 taccgtgctc agcaggccgt ggcggcagcc cgcgggacgg agatgcagat tgctgcagag
       61 gcccgtctgg cggccacaca ggaacgcctg aacagaaata ttgctgccag aagcgccgcc
      121 cagaatgcgc tgaacagtac aacggcggtg ggctcacgtc tgatgagcgg tgcgctgggg
      181 ctggttggtg gcgtacccgg actggtgatg ctgggggctg cagcatggta cacgctgtac
      241 cagaatcagg agcaggccag ggagtctgcg cgccagtatg cactgacgat agatgaaatc
      301 gcgcataaaa cgccgtcaat gtctttgcct gaagcctcag ataatgaa
//
LOCUS       DAZFWL020000607          347 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZFWL020000607 DAZFWL020000000
VERSION     DAZFWL020000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.614"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>347)
                     /locus_tag="ACHKNP_RS28705"
                     /old_locus_tag="pgaptmp_005741"
     CDS             complement(<1..>347)
                     /locus_tag="ACHKNP_RS28705"
                     /old_locus_tag="pgaptmp_005741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGU4707640.1"
                     /translation="GMPASYTLILIDGVRQGGSSDVTPNGFSAMNTGFMPPLAAIERI
                     EVIRGPMSTLYGSDAMGGVVNIITRKNADKWLSSVNAGLNLQESNKWGNSSQFNFWSS
                     GPLVDDSVSLQVRG"
BASE COUNT           85 a          103 c           80 g           79 t
ORIGIN      
        1 ccgcgtacct gcaggctgac agaatcatcc acaaggggac cactgctcca gaaattaaac
       61 tggctgctgt taccccattt gttgctttcc tgcagattca gccctgcatt gacggaagag
      121 agccatttgt ctgcattctt tctggtaatg atattcacca caccgcccat cgcatcagag
      181 ccatacagtg tggacatcgg ccccctgata acctcaatac gctcaatggc ggccagaggg
      241 ggcatgaacc cggtattcat ggcagaaaaa ccgttgggag tcacgtcact gcttccgccc
      301 tgacgaacac catcaatcag tatcagcgtg taactggctg gcattcc
//
LOCUS       DAZFWL020000608          344 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZFWL020000608 DAZFWL020000000
VERSION     DAZFWL020000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.615"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            8..>344
                     /locus_tag="ACHKNP_RS28710"
                     /old_locus_tag="pgaptmp_005742"
     CDS             8..>344
                     /locus_tag="ACHKNP_RS28710"
                     /old_locus_tag="pgaptmp_005742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HGU4707641.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSL"
BASE COUNT          107 a           69 c           55 g          113 t
ORIGIN      
        1 tgggcatatg caagtctttt ccagtgatgt ctacttcact gtaggcacta atgcattatt
       61 agcatcccaa aaagaatatt acagcgattt agttgcgttg gttgatcttg gacattcgtt
      121 cgttgttatt gatgagcatc aacatcgaaa tctcaaacca aatacagaac ctgtaaacat
      181 ccttttgagc aacaatttca tcaggatcaa taagaatatt acattatctg atttaacgca
      241 tttcctggtt tcaaatcttc atacacaaaa tgtgtattcc acacaagaac cacttaccca
      301 tgatgagata gatatcctgc ggttatgtgt ttcttactcg ttaa
//
LOCUS       DAZFWL020000609          338 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZFWL020000609 DAZFWL020000000
VERSION     DAZFWL020000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.616"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>338
                     /locus_tag="ACHKNP_RS28715"
                     /old_locus_tag="pgaptmp_005743"
     CDS             <1..>338
                     /locus_tag="ACHKNP_RS28715"
                     /old_locus_tag="pgaptmp_005743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4707642.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRA
                     QAREQFTGAV"
BASE COUNT           66 a          106 c          103 g           63 t
ORIGIN      
        1 acatcctgca ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacgg
       61 agccctgcca tgaatgggat atggccggca ttcagccttc tcccgcacag gcacagaaca
      121 ttcgggagaa aacggaggcg gtattccgtc tgttccggcc cgtacatccg gttgttgccg
      181 tatcggcccg caccggctgg gaactggata cgctggtcag tgcgctaatg acagcacttc
      241 ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgagctgcgc acggaatctg
      301 tccgcgctca ggcccgtgaa cagtttaccg gtgcggtg
//
LOCUS       DAZFWL020000610          335 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZFWL020000610 DAZFWL020000000
VERSION     DAZFWL020000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.617"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>335
                     /locus_tag="ACHKNP_RS28720"
                     /old_locus_tag="pgaptmp_005744"
     CDS             <1..>335
                     /locus_tag="ACHKNP_RS28720"
                     /old_locus_tag="pgaptmp_005744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4707643.1"
                     /translation="DPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQ"
BASE COUNT           87 a           77 c          113 g           58 t
ORIGIN      
        1 gacccgacgt cgggactgat ggcgatggcg cgccagttcc gtaacgtgac ggcagagcag
       61 attgcgtatg ttgcacagct gcagcgttcc ggagacgagg ccggggcatt gcaggcggcg
      121 aacgatatcg ccacgaaagg ctttgatgag cagacccgtc gcctgaaaga aaacatggga
      181 acactggaga cctgggcgga taaaacaggg aaggcattca aatcgatgtg ggatgccatt
      241 ctggatatcg gtcgtccgga atcctcagcg gatatgctcg ccagtgcgca gaaggcattt
      301 gatgaggcgg ataaaaaatg gcagtggtac cagag
//
LOCUS       DAZFWL020000611          334 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZFWL020000611 DAZFWL020000000
VERSION     DAZFWL020000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.618"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..166
                     /gene="xisR"
                     /locus_tag="ACHKNP_RS28725"
                     /old_locus_tag="pgaptmp_005745"
     CDS             <1..166
                     /gene="xisR"
                     /locus_tag="ACHKNP_RS28725"
                     /old_locus_tag="pgaptmp_005745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4707644.1"
                     /translation="LKKAREKSFMEGREYKHVAHDGMPWDNSPCFYNLEEIDRWIERQ
                     ASARPRRHLT"
     gene            186..>334
                     /locus_tag="ACHKNP_RS28730"
                     /old_locus_tag="pgaptmp_005746"
     CDS             186..>334
                     /locus_tag="ACHKNP_RS28730"
                     /old_locus_tag="pgaptmp_005746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HGU4707645.1"
                     /translation="MREVEMKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTSKNRR
                     VAGELR"
BASE COUNT          113 a           59 c           89 g           73 t
ORIGIN      
        1 gttaaaaaag gcccgggaaa aatcgtttat ggaaggaagg gaatataagc atgtcgctca
       61 tgacggtatg ccatgggata acagtccatg cttttataac ctggaagaaa ttgatcgctg
      121 gattgagcgc caggcatcag cgagaccaag acgtcatctt acttgactaa aagccacact
      181 aactaatgag agaagttgaa atgaaatatc cgacaggcgt ggaaaaccat ggagggaaat
      241 tacgtatctg gtttgtttat aaaggcgtaa gagtcaggga aaatctgggg gttcctgaca
      301 catcaaaaaa caggcgcgtt gcaggtgaac tacg
//
LOCUS       DAZFWL020000612          331 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZFWL020000612 DAZFWL020000000
VERSION     DAZFWL020000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.619"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>331)
                     /locus_tag="ACHKNP_RS28735"
                     /old_locus_tag="pgaptmp_005747"
     CDS             complement(<1..>331)
                     /locus_tag="ACHKNP_RS28735"
                     /old_locus_tag="pgaptmp_005747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGU4707646.1"
                     /translation="ATLVKPVPDMPSQLAARLDEVWAAIKTGLVRGLSVGFRPHEYTF
                     LDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFSAASGNRKPVVKIASSAGA
                     AAQSTTVF"
BASE COUNT           70 a          102 c           99 g           60 t
ORIGIN      
        1 aaaaacggtt gttgactgtg ccgcagcgcc ggcagaagat gcgattttca ccaccggttt
       61 ccggttgccg gacgcggcag aaaacgggcg gtcgtaagat ttaatggtcc ggatggtgca
      121 ttccgcattc gcgggcacgg tgacggcaga cacctccatc agttcccagc gcagaaaatg
      181 cagtccgcct ccgtccagaa aggtgtattc atggggacgg aagcccacgg acagccccct
      241 gaccagcccg gtcttaatgg ccgcccagac ctcatccagc cgggcagcca gttgcgacgg
      301 catatccggt acgggcttca ccagtgttgc c
//
LOCUS       DAZFWL020000613          331 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZFWL020000613 DAZFWL020000000
VERSION     DAZFWL020000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.620"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /gene="cbtA"
                     /locus_tag="ACHKNP_RS28740"
                     /old_locus_tag="pgaptmp_005748"
     CDS             <1..198
                     /gene="cbtA"
                     /locus_tag="ACHKNP_RS28740"
                     /old_locus_tag="pgaptmp_005748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HGU4707647.1"
                     /translation="DAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATGLM
                     TRDNYRTVNNITLGKYQEAKR"
     gene            195..>331
                     /locus_tag="ACHKNP_RS28745"
                     /old_locus_tag="pgaptmp_005749"
     CDS             195..>331
                     /locus_tag="ACHKNP_RS28745"
                     /old_locus_tag="pgaptmp_005749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4707648.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLTELINNVAENGYS
                     LR"
BASE COUNT           87 a           83 c           86 g           75 t
ORIGIN      
        1 gatgcagtga actttcttgt tgaaaaatac gcactggtgc gtaccgacca gccggaattc
       61 agtgcctgta cccgctctca gttaataaac agcatcgata tcctccgggc tcgccgggcg
      121 actggcctga tgacccgcga caattaccga acggtaaata acattaccct gggtaagtat
      181 caggaggcga aacgatgaaa ctggccctga cgctggaagc tgacagcgtt aacgtgcagg
      241 cactgaacat ggggcgcatt gtcgttgacg tcgatggtgt taatctcact gaactgatta
      301 ataacgtcgc tgaaaacggt tattcactcc g
//
LOCUS       DAZFWL020000614          328 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZFWL020000614 DAZFWL020000000
VERSION     DAZFWL020000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.621"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..195
                     /locus_tag="ACHKNP_RS28750"
                     /old_locus_tag="pgaptmp_005750"
     CDS             <1..195
                     /locus_tag="ACHKNP_RS28750"
                     /old_locus_tag="pgaptmp_005750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4707649.1"
                     /translation="DAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATGLM
                     TRDNYRTVNNITLGKHPEKR"
     gene            192..>328
                     /locus_tag="ACHKNP_RS28755"
                     /old_locus_tag="pgaptmp_005751"
     CDS             192..>328
                     /locus_tag="ACHKNP_RS28755"
                     /old_locus_tag="pgaptmp_005751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4707650.1"
                     /translation="MKLSLMLEADRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LR"
BASE COUNT           88 a           79 c           85 g           76 t
ORIGIN      
        1 gatgcggtga attttctcgt ggaaaaatac gcgctggtgc gtaccgacca gccgggattc
       61 agcacttgtc cccgctctca gttaataaac agtattgata tcctccgggc tcgcagggcg
      121 accggcctga tgacccgcga caattacaga acggtaaata acattaccct gggtaagcat
      181 ccggagaaac gatgaaactt tccctgatgc tggaagccga cagaattaat gtgcaggcac
      241 tgaacatggg gcgaattgtc gttgacgtcg atggtgttaa tctcactgaa ctgattaaca
      301 aggtcgctga aaacggttat tcactccg
//
LOCUS       DAZFWL020000615          324 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZFWL020000615 DAZFWL020000000
VERSION     DAZFWL020000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.622"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..312
                     /locus_tag="ACHKNP_RS28760"
                     /old_locus_tag="pgaptmp_005752"
     CDS             <1..312
                     /locus_tag="ACHKNP_RS28760"
                     /old_locus_tag="pgaptmp_005752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4707651.1"
                     /translation="PPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFTG
                     SGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTFN
                     W"
BASE COUNT           74 a           90 c           79 g           81 t
ORIGIN      
        1 ccaccgtttg caacccttgc cggcatacgc tgcagtaccg cacatatcac ggaaaaggat
       61 aacgcctggc tgtactcgct gtcgcatcag accagtgacg ttggtgaatc agaatggatt
      121 catttcacag gtagcggata tctgttacgt accgatgcgt ggtcatatcc ggttctgcgg
      181 cttaaacgcc tggggctgtc aaaaacgttc cgtcgtctgg ttatcacact tacccgacgt
      241 tatggcgtca gtctcattca tctggatgcc agcgctgaat gcctgccggg tttacccact
      301 ttcaactggt aaccaggaac aaca
//
LOCUS       DAZFWL020000616          323 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZFWL020000616 DAZFWL020000000
VERSION     DAZFWL020000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.623"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..266
                     /locus_tag="ACHKNP_RS28765"
                     /old_locus_tag="pgaptmp_005753"
     CDS             <1..266
                     /locus_tag="ACHKNP_RS28765"
                     /old_locus_tag="pgaptmp_005753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGU4707652.1"
                     /translation="APWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTDRFG
                     QVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
BASE COUNT           63 a           85 c           92 g           83 t
ORIGIN      
        1 ttgccccctg gttaccccgt gatgaaacaa cggcgctcac cgaggaggca ttcatcgggt
       61 gtatggccac catcaccggt tatcacgcag tggcgggttc cccctgtgaa ggtcgcttca
      121 cagaccgctt tgggcaggtt cattacctgt tgctggagcc ggaggccggg aaagaattca
      181 gacgaggaga caaagtgatt attgtctgcc gtctttccgg ttcccgctgg ctgggcgaac
      241 tgaatccctg gccggatgtt ctttaatccc gacatataca gaggttctca ctatgtttaa
      301 tcttccggga tggttgtagt tgc
//
LOCUS       DAZFWL020000617          320 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZFWL020000617 DAZFWL020000000
VERSION     DAZFWL020000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.624"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>320
                     /locus_tag="ACHKNP_RS28770"
                     /old_locus_tag="pgaptmp_005754"
     rRNA            <1..>320
                     /locus_tag="ACHKNP_RS28770"
                     /old_locus_tag="pgaptmp_005754"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           97 a           72 c           99 g           52 t
ORIGIN      
        1 cataggttaa tgaggcgaac cgggggaact gaaacatcta agtaccccga ggaaaagaaa
       61 tcaaccgaga ttcccccagt agcggcgagc gaacggggag cagcccagag cctgaatcag
      121 tatgtgtgtt agtggaagcg tctggaaagg cgcgcgatac agggtgacag ccccgtacac
      181 aaaaatgcac atgctgtgag ctcgatgagt agggcgggac acgtggtatc ctgtctgaat
      241 atggggggac catcctccaa ggctaaatac tcctgactga ccgatagtga accagtaccg
      301 tgagggaaag gcgaaaagaa
//
LOCUS       DAZFWL020000618          315 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZFWL020000618 DAZFWL020000000
VERSION     DAZFWL020000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.625"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>315
                     /locus_tag="ACHKNP_RS28775"
                     /old_locus_tag="pgaptmp_005755"
     CDS             <1..>315
                     /locus_tag="ACHKNP_RS28775"
                     /old_locus_tag="pgaptmp_005755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4707653.1"
                     /translation="RGGKSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTAQG
                     ELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGE
                     RA"
BASE COUNT           94 a           68 c          110 g           43 t
ORIGIN      
        1 ccggggtgga aaatcagaga cggaaaaagc ggaagacagt ttttcccggc tgctgaagca
       61 gcagaaagag caactggcac tggtggggca gaatacagag ctggcgaagc tgaaatacca
      121 gactgcgcag ggcgaactga aaaccctgac ggagatgcag aagcaggagc tgctgcgtaa
      181 tgcggccctg attgaccagc aaaaaatccg ggaacagttg cgatcccggg aagagacact
      241 gaagaatgag aatgcggctg cgcgtgcgtc gaatgatgct gaactgctgg ggtacgggca
      301 gggggaacga gccag
//
LOCUS       DAZFWL020000619          314 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZFWL020000619 DAZFWL020000000
VERSION     DAZFWL020000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.626"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>314
                     /locus_tag="ACHKNP_RS28780"
                     /old_locus_tag="pgaptmp_005756"
     CDS             <1..>314
                     /locus_tag="ACHKNP_RS28780"
                     /old_locus_tag="pgaptmp_005756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HGU4707654.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VY"
BASE COUNT           80 a           70 c           88 g           76 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acat
//
LOCUS       DAZFWL020000620          313 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZFWL020000620 DAZFWL020000000
VERSION     DAZFWL020000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.627"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..276
                     /locus_tag="ACHKNP_RS28785"
                     /old_locus_tag="pgaptmp_005757"
     CDS             <1..276
                     /locus_tag="ACHKNP_RS28785"
                     /old_locus_tag="pgaptmp_005757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4707655.1"
                     /translation="LRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRALMIESATEG
                     QVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
BASE COUNT           80 a           64 c           87 g           82 t
ORIGIN      
        1 ttaagggttg gtgatgctaa gaaattcgcg gccagacttg gtgtatcgcc atcttactta
       61 tcgcaaatgg cgtctggacg aacagctata tctccaaccc gcgcccttat gatcgaatct
      121 gcgacggaag gccaagtaag tagggcggag ctacgacctc atgattggga gcttatttgg
      181 cctgagtatg cgagcggcat tcgtttgggg caaacacatg tagttcatgc tgaaggtgat
      241 tgtagtgcat gcttatctga tggagttgat tcatgaaaat caagcatgaa cacatccgca
      301 tggcgatgaa tgt
//
LOCUS       DAZFWL020000621          308 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZFWL020000621 DAZFWL020000000
VERSION     DAZFWL020000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.628"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>308
                     /locus_tag="ACHKNP_RS28790"
                     /old_locus_tag="pgaptmp_005758"
     CDS             <1..>308
                     /locus_tag="ACHKNP_RS28790"
                     /old_locus_tag="pgaptmp_005758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4707656.1"
                     /translation="FNCFFHPLVGLPPVYAAGLAAMQGHHIQANSTYFFRNGGRPSGV
                     IEVPGSITEENAKKLKGNWDSGYTGENAGKTAILSNGAKYSPTTFSPVDAQTVEQLKM
                     T"
BASE COUNT           83 a           71 c           94 g           60 t
ORIGIN      
        1 tttaactgtt tttttcatcc gcttgtgggg ctgccgccgg tgtatgccgc aggactggcc
       61 gccatgcagg ggcatcatat tcaggcaaat tcgacgtatt ttttcagaaa tggcgggcga
      121 ccatcaggcg tgattgaggt ccccggcagt attacggaag aaaacgcgaa aaaactgaag
      181 gggaactggg acagcggata tacgggcgaa aatgccggga agacggccat actgagcaac
      241 ggggcaaaat acagccccac gacgttttca ccggtggatg cgcagacggt ggaacaactg
      301 aaaatgac
//
LOCUS       DAZFWL020000622          303 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZFWL020000622 DAZFWL020000000
VERSION     DAZFWL020000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.629"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT           90 a           57 c           46 g          110 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tca
//
LOCUS       DAZFWL020000623          302 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZFWL020000623 DAZFWL020000000
VERSION     DAZFWL020000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.630"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>302)
                     /locus_tag="ACHKNP_RS28795"
                     /old_locus_tag="pgaptmp_005759"
     CDS             complement(<1..>302)
                     /locus_tag="ACHKNP_RS28795"
                     /old_locus_tag="pgaptmp_005759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4707657.1"
                     /translation="QDIAKMRLRLMQTDVQGIRREKRQGDTARLCRRPNAQQNRIQFF
                     ELWLNSKLRHGNTVVLKIRNPRGQIKELRILDWNRVEPLVADDGDVFYRITPDRNC"
BASE COUNT           63 a           90 c           78 g           71 t
ORIGIN      
        1 cacagttccg gtccggcgtg atgcggtaga acacgtcgcc gtcatccgcc accagaggtt
       61 caacccggtt ccagtccaga atacgcagtt ctttgatctg cccccggggg ttgcggattt
      121 tcagcaccac cgtattgccg tgacgcaatt tggaattcag ccacagttca aaaaactgga
      181 tgcgattctg ctgcgcatta ggacgacgac agagacgggc agtatctccc tgccgctttt
      241 cccggcgtat tccctgtaca tcagtctgca tcaggcgaag ccgcattttg gcaatatcct
      301 gg
//
LOCUS       DAZFWL020000624          299 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZFWL020000624 DAZFWL020000000
VERSION     DAZFWL020000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.631"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>299)
                     /locus_tag="ACHKNP_RS28800"
                     /old_locus_tag="pgaptmp_005760"
     CDS             complement(<1..>299)
                     /locus_tag="ACHKNP_RS28800"
                     /old_locus_tag="pgaptmp_005760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU4707658.1"
                     /translation="WWLVCRGEIHKFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAEIE
                     MPDFVREDDWWRNGQNLYLDNLEATGLYQVPLSAAQPGDVLLCCFGSSVPNHAAI"
BASE COUNT           81 a          103 c           68 g           47 t
ORIGIN      
        1 aaatagcggc gtgattcggc accgatgaac caaaacagca cagcagcaca tcgcccggct
       61 gcgccgctga caacggcacc tgatacagcc ctgtggcctc cagattatcc agatagagat
      121 tctgaccgtt acgccaccag tcatcctcac gcacaaaatc cggcatctca atctccgcca
      181 gatgataagc atcccggaac agtgtgtaac agtccgtcac accgtgctca aagcgccgcc
      241 cggtgagatg cggcacacag cggaacttat gaatctcacc ccggcagacc agccaccac
//
LOCUS       DAZFWL020000625          299 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZFWL020000625 DAZFWL020000000
VERSION     DAZFWL020000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.632"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>299)
                     /locus_tag="ACHKNP_RS28805"
                     /old_locus_tag="pgaptmp_005761"
     CDS             complement(<1..>299)
                     /locus_tag="ACHKNP_RS28805"
                     /old_locus_tag="pgaptmp_005761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HGU4707659.1"
                     /translation="LKALPQSNIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSL
                     NELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQ"
BASE COUNT          110 a           66 c           36 g           87 t
ORIGIN      
        1 tttgttttaa ccattcccgt aaattaatat acattacacc agagttaaaa tagctaccgt
       61 taaaatcctc attacacaaa cgctcagcgc ttttactttg catagcatct acatcaatta
      121 ccactgcccc atattcatct ttaaactcta atgctattaa ctcgttcaat gaacccttac
      181 agacgatatc agcatcaaga tataataatg aatccaatcg cgcagaaaaa taatcaaatg
      241 aaagcaaacg ataataaata gatactggcc agatatttga ttgaggtagc gccttcaat
//
LOCUS       DAZFWL020000626          298 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZFWL020000626 DAZFWL020000000
VERSION     DAZFWL020000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.633"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>298)
                     /locus_tag="ACHKNP_RS28810"
                     /old_locus_tag="pgaptmp_005762"
     CDS             complement(<1..>298)
                     /locus_tag="ACHKNP_RS28810"
                     /old_locus_tag="pgaptmp_005762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707660.1"
                     /translation="SFFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPK
                     ARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEG"
BASE COUNT           66 a           94 c           79 g           59 t
ORIGIN      
        1 ccccttcgtc tgccgtgctg atctcctgag aaaccacgcg agaccccacg cgcatttccc
       61 cgtacagaac aggcagaaca ttgccctggg caaccatgtt atccagtgag gagaaatagg
      121 tgttctgctt accgttatcc gttgtctgtg tacggggagt tctggctttc ggtgccagca
      181 tctgcgccac accaccgagc accatactgg caccgagaga aaacaggatg ccggtcatac
      241 caccggcccc aatggctgcc ccccatgctg caagggtggc tccggcggta aagaatga
//
LOCUS       DAZFWL020000627          295 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZFWL020000627 DAZFWL020000000
VERSION     DAZFWL020000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.634"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>295
                     /locus_tag="ACHKNP_RS28815"
                     /old_locus_tag="pgaptmp_005763"
     CDS             <1..>295
                     /locus_tag="ACHKNP_RS28815"
                     /old_locus_tag="pgaptmp_005763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4707661.1"
                     /translation="LGIYLYTDAPVPDGVAMTGTGNPFLMSYFTQTTDGRVNLMHHRK
                     AGNTKLGEFGDYGNDWQTLELVFTAGSATVTPKLNGVAGPAFQVIKDSLTLGLN"
BASE COUNT           68 a           70 c           92 g           65 t
ORIGIN      
        1 ctggggattt atctgtatac ggatgctccc gttcctgatg gtgtggcgat gacgggtacc
       61 ggtaatccgt tcctgatgtc gtacttcact cagaccactg acggcagagt gaatctgatg
      121 catcacagga aagccggaaa cacgaagctg ggggagttcg gcgattacgg taacgactgg
      181 cagacgctgg agctggtgtt caccgccggc agtgccacgg ttactccgaa actgaatgga
      241 gtggctggcc cggcattcca ggttataaaa gacagtctga cactgggact gaatg
//
LOCUS       DAZFWL020000628          291 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZFWL020000628 DAZFWL020000000
VERSION     DAZFWL020000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.635"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..166
                     /locus_tag="ACHKNP_RS28820"
                     /old_locus_tag="pgaptmp_005764"
                     /pseudo
     CDS             1..166
                     /locus_tag="ACHKNP_RS28820"
                     /old_locus_tag="pgaptmp_005764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          119 a           53 c           41 g           78 t
ORIGIN      
        1 tatcagcaag aattccaaaa acaatgttat aagcaagtta aagcctttac gacactctct
       61 cgatacaata aaattcaaac atgggcagaa atgtctgaat atcagttcga agtatttcaa
      121 tacgagacgc ttaaccccaa aaaaatgtca cacacgcctt atttaaagag gccacttcct
      181 aatgaaaaag atataaatta tggggtggaa atagaaatac catctggcaa aagaattcgc
      241 ttatcaaatc attatcagaa tataatacca taatattgat tattagcact t
//
LOCUS       DAZFWL020000629          287 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZFWL020000629 DAZFWL020000000
VERSION     DAZFWL020000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.636"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>287
                     /locus_tag="ACHKNP_RS28825"
                     /old_locus_tag="pgaptmp_005765"
     rRNA            <1..>287
                     /locus_tag="ACHKNP_RS28825"
                     /old_locus_tag="pgaptmp_005765"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a           67 c           80 g           53 t
ORIGIN      
        1 cctgcttcca ggaaaagcct ctaagcatca ggtaacatca aatcgtaccc caaaccgaca
       61 caggtggtca ggtagagaat accaaggcgc ttgagagaac tcgggtgaag gaactaggca
      121 aaatggtgcc gtaacttcgg gagaaggcac gctgatatgt aggtgaagtc cctcgcggat
      181 ggagctgaaa tcagtcgaag ataccagctg gctgcaactg tttattaaaa acacagcact
      241 gtgcaaacac gaaagtggac gtatacggtg tgacgcctgc ccggtgc
//
LOCUS       DAZFWL020000630          286 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZFWL020000630 DAZFWL020000000
VERSION     DAZFWL020000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.637"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>286
                     /locus_tag="ACHKNP_RS28830"
                     /old_locus_tag="pgaptmp_005766"
     CDS             <1..>286
                     /locus_tag="ACHKNP_RS28830"
                     /old_locus_tag="pgaptmp_005766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4707662.1"
                     /translation="LAARKARAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWD
                     ALNVYCSNGWVEIDNNIAENALRGVAVGRKNWMFAGSDSGGEHAAVLYSLI"
BASE COUNT           77 a           68 c           88 g           53 t
ORIGIN      
        1 tctggcggca agaaaagcca gagccgcgcc actgatgcag tcactgtatg actggataca
       61 gcaacagatg aaaacactgt cgcgtcactc agatacggca aaagcgttcg catacctgct
      121 gaaacagtgg gatgcactga acgtgtactg cagtaatggc tgggtggaaa tcgacaacaa
      181 catcgcagag aacgccttac ggggagtggc cgtaggccgg aaaaactgga tgttcgcggg
      241 ttccgacagc ggtggtgaac atgcggcggt gttgtactcg ctgatc
//
LOCUS       DAZFWL020000631          286 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZFWL020000631 DAZFWL020000000
VERSION     DAZFWL020000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.638"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>286
                     /locus_tag="ACHKNP_RS28835"
                     /old_locus_tag="pgaptmp_005767"
     CDS             <1..>286
                     /locus_tag="ACHKNP_RS28835"
                     /old_locus_tag="pgaptmp_005767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HGU4707663.1"
                     /translation="DTYISRGPVLYAIELESDILIKKTLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTF"
BASE COUNT           94 a           54 c           46 g           92 t
ORIGIN      
        1 tgacacttat atttccagag gtcccgtttt atatgcaatt gagcttgaga gcgatatttt
       61 aatcaaaaaa actctgctga acgggaaata ttttgactca ggatacatac cctgtagtag
      121 agctgatgaa tctatccagt tctcttatgc tgatatggaa tctttttcat attcaacctc
      181 accagagggc aaacaataca ttgaaggctg tttttattta aacaagacta aaatagtcag
      241 aaaacttata ccatttggtc aaaccattct tcgcaaagta actttc
//
LOCUS       DAZFWL020000632          284 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZFWL020000632 DAZFWL020000000
VERSION     DAZFWL020000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.639"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>284
                     /locus_tag="ACHKNP_RS28840"
                     /old_locus_tag="pgaptmp_005768"
     CDS             <1..>284
                     /locus_tag="ACHKNP_RS28840"
                     /old_locus_tag="pgaptmp_005768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure C-terminal
                     domain-containing protein"
                     /protein_id="HGU4707664.1"
                     /translation="ADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNP
                     LALNNVMSEQKKTWAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAA"
BASE COUNT           81 a           67 c          101 g           35 t
ORIGIN      
        1 aggcggataa attcgcacag cagcaacggg caaaacgggc cgccattgat gcgaaaagcc
       61 gggggctgac tgaccggcag gcagaacggg aagccacgga acagcgcctg aaggaacagt
      121 atggcgataa tccgctggcg ctgaataacg tcatgtcaga gcagaaaaag acctgggcgg
      181 ctgaagacca gcttcgcggg agctggatgg caggcctgaa gtccggctgg agtgagtggg
      241 aagagagcgc cacggacagt atgtcgcagg taaaaagtgc agcc
//
LOCUS       DAZFWL020000633          284 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZFWL020000633 DAZFWL020000000
VERSION     DAZFWL020000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.640"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          115 a           54 c           42 g           73 t
ORIGIN      
        1 gtattacctc attacagtaa tagggtaatg attcagaata cagtaagact ctcttaaact
       61 gaggaccaga acgaattaca ataaaaaaca cacctcatat aattgagttt aagaaagcac
      121 cattcaatta cttacaggaa ataatctgat cttcacaaaa gtcagcattg aatagccaca
      181 ggcaacatcg ctattagtat tgccgtacca ttaagtaaaa acagagactc tgcagagaga
      241 gcctacagat atttaatctc acaataagaa gattatttca taca
//
LOCUS       DAZFWL020000634          281 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZFWL020000634 DAZFWL020000000
VERSION     DAZFWL020000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.641"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>281)
                     /locus_tag="ACHKNP_RS28845"
                     /old_locus_tag="pgaptmp_005769"
     rRNA            complement(<1..>281)
                     /locus_tag="ACHKNP_RS28845"
                     /old_locus_tag="pgaptmp_005769"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           57 a           96 c           57 g           71 t
ORIGIN      
        1 agccttgatt ttccggattt gcctggaaaa ccagcctaca cgcttaaacc gggacaaccg
       61 tcgcccggcc aacatagcct tctccgtccc cccttcgcag taacaccaag tacaggaata
      121 ttaacctgtt tcccatcgac tacgcctttc ggcctcgcct taggggtcga ctcaccctgc
      181 cccgattaac gttggacagg aacccttggt cttccggcga gcgggctttt cacccgcttt
      241 atcgttactt atgtcagcat tcgcacttct gatacctcca g
//
LOCUS       DAZFWL020000635          276 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZFWL020000635 DAZFWL020000000
VERSION     DAZFWL020000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.642"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>276
                     /locus_tag="ACHKNP_RS28850"
                     /old_locus_tag="pgaptmp_005770"
     CDS             <1..>276
                     /locus_tag="ACHKNP_RS28850"
                     /old_locus_tag="pgaptmp_005770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGU4707665.1"
                     /translation="SDLSNTIFSNGEINNLFIKKSNIFGASFTNTRIKNLLCEDIMPG
                     RWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNV"
BASE COUNT          101 a           54 c           43 g           78 t
ORIGIN      
        1 aagcgactta tcaaacacca tcttttcaaa tggagaaatt aataatctct tcattaaaaa
       61 aagcaacata tttggagcat cattcactaa taccagaata aaaaatttgc tctgtgaaga
      121 tattatgcct ggtcgatgga ccactcaact tgtcaataag cacttaggtt acagatacac
      181 aggagtattc aaaaccctgg caagtattga tgacaaaccg tcccgtttcg aaattttgat
      241 accattggta caaactttag ttcgcgacaa tgtaaa
//
LOCUS       DAZFWL020000636          276 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZFWL020000636 DAZFWL020000000
VERSION     DAZFWL020000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.643"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT           56 a           88 c           54 g           78 t
ORIGIN      
        1 gatgatcttt ccctaatctc acataggatc agatctcgtc tgatcgttcc ccctgcgctc
       61 actaccatgg ctctttacca cagcagccgg ggttgtttgg gaccgacttc cgacagcaga
      121 tcccggtggg cctgccacca tctcttcaca agcttatgct tattacatgc aaaacgtccc
      181 gttttgcatc tttgtgccta cggcatccct gtgatctttc cctgaatctg cccctgagaa
      241 cctgaatcaa acgtctgact cgcagaacca tctgtt
//
LOCUS       DAZFWL020000637          276 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZFWL020000637 DAZFWL020000000
VERSION     DAZFWL020000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.644"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>276)
                     /locus_tag="ACHKNP_RS28855"
                     /old_locus_tag="pgaptmp_005771"
     CDS             complement(<1..>276)
                     /locus_tag="ACHKNP_RS28855"
                     /old_locus_tag="pgaptmp_005771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HGU4707666.1"
                     /translation="QVYPYVVPLLDGRPSVALPWVVFSLISSVSADVMGGQAESSVSV
                     QIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENRCYRAT"
BASE COUNT           60 a           96 c           73 g           47 t
ORIGIN      
        1 gggttgcacg gtaacagcgg ttttccggaa tatagtcctg catttcactg acggatcccg
       61 gggccagcag cattatggct tcacgggcgt cctgacgtat ctgacgcgcc tgcgtcacag
      121 tcccggcata aacgtctatc tgcaccgaca ctgaggactc cgcctgcccg cccatcacgt
      181 ccgccgacac cgatgaaatc aggctgaaaa ccacccacgg aagcgccacc gacggcctgc
      241 catccagcag ggggaccaca tacgggtaca cctgcc
//
LOCUS       DAZFWL020000638          273 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZFWL020000638 DAZFWL020000000
VERSION     DAZFWL020000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.645"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            19..95
                     /locus_tag="ACHKNP_RS28860"
                     /old_locus_tag="pgaptmp_005772"
     tRNA            19..95
                     /locus_tag="ACHKNP_RS28860"
                     /old_locus_tag="pgaptmp_005772"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53..55,aa:Ile,seq:gat)
     gene            138..213
                     /locus_tag="ACHKNP_RS28865"
                     /old_locus_tag="pgaptmp_005773"
     tRNA            138..213
                     /locus_tag="ACHKNP_RS28865"
                     /old_locus_tag="pgaptmp_005773"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:171..173,aa:Ala,seq:tgc)
BASE COUNT           71 a           62 c           73 g           67 t
ORIGIN      
        1 gagcagtaaa acctctacag gcttgtagct caggtggtta gagcgcaccc ctgataaggg
       61 tgaggtcggt ggttcaagtc cactcaggcc taccaaattt gcacggcaaa tttgaagagg
      121 ttttaactac atgttatggg gctatagctc agctgggaga gcgcctgctt tgcacgcagg
      181 aggtctgcgg ttcgatcccg catagctcca ccatctctgt agtgattaaa taaaaaatac
      241 ttcagagtgt acctgcaaag gttcactgcg aag
//
LOCUS       DAZFWL020000639          267 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZFWL020000639 DAZFWL020000000
VERSION     DAZFWL020000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.646"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            122..197
                     /locus_tag="ACHKNP_RS28870"
                     /old_locus_tag="pgaptmp_005774"
     tRNA            122..197
                     /locus_tag="ACHKNP_RS28870"
                     /old_locus_tag="pgaptmp_005774"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Glu,seq:ttc)
BASE COUNT           70 a           65 c           67 g           65 t
ORIGIN      
        1 taaatagcaa ggcgtttacg cgttgggagt gaggctgaag agaataaggc cgttcgcttt
       61 ctattaatga aagctcaccc tacacgaaaa tatcacgcaa cgcgtgataa gcaattttcg
      121 tgtccccttc gtctagaggc ccaggacacc gccctttcac ggcggtaaca ggggttcgaa
      181 tcccctaggg gacgccactt gctggtttgt gagtgaaagt cgccgacccc aatatctcaa
      241 aacagactgt taagtcttgt ttgagat
//
LOCUS       DAZFWL020000640          262 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZFWL020000640 DAZFWL020000000
VERSION     DAZFWL020000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.647"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..159
                     /locus_tag="ACHKNP_RS28875"
                     /old_locus_tag="pgaptmp_005775"
     CDS             <1..159
                     /locus_tag="ACHKNP_RS28875"
                     /old_locus_tag="pgaptmp_005775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4707667.1"
                     /translation="TGYWFLQLLDRVSPSQWAAIGVLGSLLFGLLTYLTNLYFKIRED
                     RRKAARGE"
     gene            164..>262
                     /locus_tag="ACHKNP_RS28880"
                     /old_locus_tag="pgaptmp_005776"
     CDS             164..>262
                     /locus_tag="ACHKNP_RS28880"
                     /old_locus_tag="pgaptmp_005776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4707668.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDE"
BASE COUNT           50 a           62 c           86 g           64 t
ORIGIN      
        1 acgggctact ggttcctgca gttgctggac agggtttccc cgtctcagtg ggcggcaata
       61 ggcgtgctgg ggagtctgct gtttggtctg ctgacgtacc tgacgaacct gtatttcaaa
      121 atcagagagg acaggcgtaa ggcggcacgg ggagagtaag ctgatgagca ggaaactccg
      181 ctatggttta tcggctgccg ttctggcgct gattgccgca ggtgcttctg cgcctgaaat
      241 cctcgaccag tttctggatg aa
//
LOCUS       DAZFWL020000641          262 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZFWL020000641 DAZFWL020000000
VERSION     DAZFWL020000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.648"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..262
                     /locus_tag="ACHKNP_RS28885"
                     /old_locus_tag="pgaptmp_005777"
                     /pseudo
     CDS             1..262
                     /locus_tag="ACHKNP_RS28885"
                     /old_locus_tag="pgaptmp_005777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT           89 a           45 c           45 g           83 t
ORIGIN      
        1 actatcgaaa ttccacagac agtaaatggt ggttacgatt tttcacacct tagcctgaaa
       61 ggtatcgtga ttaaagatga agatttatcc aattcgaatt ttgcaggttg ctgactacaa
      121 aacgctattt tccaggactg taatatgtat aaaacgaatt tttattacgc cataatggaa
      181 aaaatacttt ttgataattg tattctcgat gactcaaatt tcgctcagat aaaaatggcc
      241 gacggaactc taaatgcatg ct
//
LOCUS       DAZFWL020000642          261 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZFWL020000642 DAZFWL020000000
VERSION     DAZFWL020000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.649"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>261)
                     /locus_tag="ACHKNP_RS28890"
                     /old_locus_tag="pgaptmp_005778"
     CDS             complement(<1..>261)
                     /locus_tag="ACHKNP_RS28890"
                     /old_locus_tag="pgaptmp_005778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HGU4707669.1"
                     /translation="GHADAIKAYCGVLGNSNLKRGEIIRMLEARNYDGAPGLLLAYQN
                     GDINTIQSFFDSLIMLDISKDFIEELLTAKHYDFTGLSLAIS"
BASE COUNT           83 a           59 c           34 g           85 t
ORIGIN      
        1 gactgatcgc taatgataac cccgtaaaat cataatgttt tgcagtcaat aactcttcga
       61 taaaatcctt tgatatatct aacattatta gtgagtcaaa aaaagactga atcgtattaa
      121 tatctccatt ttgatacgca agaagtaatc ctggtgcacc atcataattt ctcgcctcaa
      181 gcatcctgat tatctcacct cttttcaaat tactattgcc aagtacgcca caatatgcct
      241 ttattgcgtc tgcatgacca t
//
LOCUS       DAZFWL020000643          261 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZFWL020000643 DAZFWL020000000
VERSION     DAZFWL020000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.650"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>261
                     /locus_tag="ACHKNP_RS28895"
                     /old_locus_tag="pgaptmp_005779"
     CDS             <1..>261
                     /locus_tag="ACHKNP_RS28895"
                     /old_locus_tag="pgaptmp_005779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4707670.1"
                     /translation="VLDIGRPDTAQEMLIKAEAAFKKADDIWNLRKDDYFVNDEARAR
                     YWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEASRLKYT"
BASE COUNT           74 a           57 c           80 g           50 t
ORIGIN      
        1 gtgctggata ttggtcgtcc tgataccgcg caggagatgc tgattaaggc agaggctgcg
       61 tttaagaaag cagacgacat ctggaatctg cgcaaggatg attattttgt taacgatgaa
      121 gcgcgggcgc gttactggga tgatcgtgaa aaggcccgtc ttgcgcttga agccgcccga
      181 aagaaggctg agcagcagac tcaacaggac aaaaatgcgc agcagcagag cgataccgaa
      241 gcgtcacggc tgaaatatac c
//
LOCUS       DAZFWL020000644          259 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZFWL020000644 DAZFWL020000000
VERSION     DAZFWL020000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.652"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>259
                     /locus_tag="ACHKNP_RS28900"
                     /old_locus_tag="pgaptmp_005780"
     CDS             <1..>259
                     /locus_tag="ACHKNP_RS28900"
                     /old_locus_tag="pgaptmp_005780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU4707671.1"
                     /translation="GAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPL
                     TEPQKAGIASFCPYNIGPGKCFPSTFYKRLNAGDRRGACEAI"
BASE COUNT           64 a           57 c           80 g           58 t
ORIGIN      
        1 ggcgccatca tggtggatgg taagcctgtg attcctggca tgaagctgtc gaaggaaaaa
       61 tgcgaccggg ttaacgctat cgaacgggat aaggcgctgg catgggtgga gcgtaatata
      121 aaagttccac tgaccgagcc acaaaaagca ggtatcgcgt cattttgccc ctataacatt
      181 ggccccggta agtgtttccc gtcgacgttt tataagcggc ttaatgcagg cgatcgcagg
      241 ggagcgtgtg aggcgattc
//
LOCUS       DAZFWL020000645          258 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZFWL020000645 DAZFWL020000000
VERSION     DAZFWL020000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.653"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>258
                     /locus_tag="ACHKNP_RS28905"
                     /old_locus_tag="pgaptmp_005781"
     CDS             <1..>258
                     /locus_tag="ACHKNP_RS28905"
                     /old_locus_tag="pgaptmp_005781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4707672.1"
                     /translation="EREGGALTEVACWAHARRGFADLYKISKDPRAAIAVKKIAGLYR
                     LEKKISSRPVEKIRQWRQRYARPILEDLWSWLEEQEPQCSPG"
BASE COUNT           65 a           63 c           81 g           49 t
ORIGIN      
        1 gaacgtgaag gtggtgcact gacagaagtt gcgtgctggg cccatgcccg caggggcttc
       61 gccgacctgt ataaaatcag taaagatcca cgggctgcca tagccgtgaa gaaaatcgcg
      121 gggttgtacc gtcttgagaa gaagatcagt agccgccccg tggaaaaaat ccgccagtgg
      181 cgacagcgtt atgcccgtcc gatactggaa gatctgtggt catggcttga agagcaggaa
      241 ccgcaatgtt ctccggga
//
LOCUS       DAZFWL020000646          257 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZFWL020000646 DAZFWL020000000
VERSION     DAZFWL020000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.654"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>257)
                     /locus_tag="ACHKNP_RS28910"
                     /old_locus_tag="pgaptmp_005782"
     CDS             complement(<1..>257)
                     /locus_tag="ACHKNP_RS28910"
                     /old_locus_tag="pgaptmp_005782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4707673.1"
                     /translation="PHTHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTR
                     WVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLC"
BASE COUNT           51 a           66 c           70 g           70 t
ORIGIN      
        1 acacagcggt agcgtgaaaa tatcgtggga ttttgttccc attccaccct gaccatgacc
       61 aatcagatga tgaggatcgt ccgctggctt accacaacac gcacacggct gtgtctttac
      121 ccagcgtgtg tatttctcat ttacccagcg gcgacgttta ggtcgtttca tgaaggattc
      181 cggagactcc ggatcaacgg caatgctgac caccgtcttt tcctgtggtg ggttctgttg
      241 ctggtgggtg tgaggcg
//
LOCUS       DAZFWL020000647          252 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZFWL020000647 DAZFWL020000000
VERSION     DAZFWL020000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.655"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            53..>252
                     /locus_tag="ACHKNP_RS28915"
                     /old_locus_tag="pgaptmp_005783"
     CDS             53..>252
                     /locus_tag="ACHKNP_RS28915"
                     /old_locus_tag="pgaptmp_005783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4707674.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSASGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSG"
BASE COUNT           47 a           71 c           81 g           53 t
ORIGIN      
        1 catctgcctt tacggggatt tgcaacgatt tggccgccgc atcgaccttc gtgtgaaaac
       61 gggggctgaa gccatccgcg cactggccac acagctcccg gcgtttcgtc agaaactgag
      121 cgacggctgg tatcaggtac ggattgccgg gcgggacgtc agcgcgtccg ggttaacggc
      181 gcagttacat gagactctgc ctgatggcgc tgtgattcat attgttccca gagtcgccgg
      241 ggccaagtca gg
//
LOCUS       DAZFWL020000648          252 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZFWL020000648 DAZFWL020000000
VERSION     DAZFWL020000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.656"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>252
                     /locus_tag="ACHKNP_RS28920"
                     /old_locus_tag="pgaptmp_005784"
     CDS             <1..>252
                     /locus_tag="ACHKNP_RS28920"
                     /old_locus_tag="pgaptmp_005784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707675.1"
                     /translation="TFNAYLSQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDV
                     VWPYTNSDVVADNEGVGFRYSFSALKDRHTAVEVSYVDP"
BASE COUNT           51 a           61 c           81 g           59 t
ORIGIN      
        1 gacctttaat gcgtacctgt cacaacagcg taaggcgtgg gacgttctca gtgatttctg
       61 ctcggcgatg cgctgtatgc cggtatggaa cgggcagacg ctgacgtttg tgcaggaccg
      121 tccgtcagat gtggtgtggc cctacacaaa cagtgatgtg gtggctgaca atgaaggtgt
      181 gggattccgc tacagtttca gcgccctgaa ggaccgtcat actgcggtgg aggtcagtta
      241 cgtcgacccg ca
//
LOCUS       DAZFWL020000649          250 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZFWL020000649 DAZFWL020000000
VERSION     DAZFWL020000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.657"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>250
                     /gene="espL"
                     /locus_tag="ACHKNP_RS28925"
                     /old_locus_tag="pgaptmp_005785"
     CDS             <1..>250
                     /gene="espL"
                     /locus_tag="ACHKNP_RS28925"
                     /old_locus_tag="pgaptmp_005785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE stator protein
                     EspL"
                     /protein_id="HGU4707676.1"
                     /translation="ELVLMQDIMNKVHSSLTNALHSLDTSSRINWDDLLNEVVRETLS
                     HNNIVGAIKITKNPDIKLDPGEANNIQLINDADAPLNKI"
BASE COUNT           90 a           43 c           44 g           73 t
ORIGIN      
        1 cgagttggtc ttaatgcagg acataatgaa taaggttcat tcgtcactga ctaatgcatt
       61 acattcttta gacacatcat caagaattaa ttgggatgat ttacttaatg aagtcgtaag
      121 ggaaacgctt tcccataaca atattgttgg agctattaag atcactaaaa acccagacat
      181 taaacttgat cctggagagg ctaataatat acagcttatt aatgatgcag acgcaccact
      241 taataagata
//
LOCUS       DAZFWL020000650          249 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZFWL020000650 DAZFWL020000000
VERSION     DAZFWL020000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.658"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /locus_tag="ACHKNP_RS28930"
                     /old_locus_tag="pgaptmp_005786"
     CDS             <1..198
                     /locus_tag="ACHKNP_RS28930"
                     /old_locus_tag="pgaptmp_005786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4707677.1"
                     /translation="LWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEET
                     TRPTLKELAAKLSGGVNGYSR"
     gene            182..>249
                     /locus_tag="ACHKNP_RS28935"
                     /old_locus_tag="pgaptmp_005787"
     CDS             182..>249
                     /locus_tag="ACHKNP_RS28935"
                     /old_locus_tag="pgaptmp_005787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4707678.1"
                     /translation="MVTVAELQALRQARLDLLTGKRV"
BASE COUNT           60 a           61 c           80 g           48 t
ORIGIN      
        1 ctgtgggata acaaaaagcg gcgtaacgaa gcgctggact gcctggtgta tgcctacgcg
       61 gcattacgtg tgtccgtgca acgctggcag cttgatctgg ctgtactggc aaaatcccgg
      121 gaagaagaga cgacccggcc aaccctgaaa gaactggcag cgaagctgtc cggaggagtg
      181 aatggttaca gtcgctgaac tgcaggcgct gcgtcaggcg cgccttgatt tattaaccgg
      241 taaacgggt
//
LOCUS       DAZFWL020000651          248 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZFWL020000651 DAZFWL020000000
VERSION     DAZFWL020000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.denovo.659"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>248
                     /locus_tag="ACHKNP_RS28940"
                     /old_locus_tag="pgaptmp_005788"
     CDS             <1..>248
                     /locus_tag="ACHKNP_RS28940"
                     /old_locus_tag="pgaptmp_005788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="IrgA-homologue adhesin (Iha); TonB-dependent; acts
                     as a specific receptor of enterobactin; fur-regulated;
                     urovirulence factor; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU4707679.1"
                     /translation="DTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYD
                     NRDGQLGSLTGGYDRTLRYERNKISAGYDHTFTFGTWKS"
BASE COUNT           63 a           57 c           70 g           58 t
ORIGIN      
        1 gatacagcag ccacgcgtat tccttatccc acggagtcac agaattataa tcttggtgca
       61 cgtcttgact ggaaggcgtc ggagcaggat gtgctctggt ttgatatgga taccacccgg
      121 cagcgttatg ataaccggga tgggcaactg gggagtctga cggggggata tgaccggacc
      181 ctgcgctatg agcgaaacaa aatttcagct ggctatgatc atactttcac cttcggaaca
      241 tggaaatc
//
LOCUS       DAZFWL020000652         6658 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.guided.203, whole genome shotgun sequence.
ACCESSION   DAZFWL020000652 DAZFWL020000000
VERSION     DAZFWL020000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.guided.203"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..654
                     /locus_tag="ACHKNP_RS28945"
                     /old_locus_tag="pgaptmp_005789"
     CDS             <1..654
                     /locus_tag="ACHKNP_RS28945"
                     /old_locus_tag="pgaptmp_005789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HGU4707680.1"
                     /translation="LHGANRLGSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQ
                     AAGVEQRLKDLVNQDGGENWAKIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERF
                     KRVRITDTSSVFNTDLLYTIELGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDD
                     VNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            647..1381
                     /gene="frdB"
                     /locus_tag="ACHKNP_RS28950"
                     /old_locus_tag="pgaptmp_005790"
     CDS             647..1381
                     /gene="frdB"
                     /locus_tag="ACHKNP_RS28950"
                     /old_locus_tag="pgaptmp_005790"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGU4707681.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1392..1787
                     /gene="frdC"
                     /locus_tag="ACHKNP_RS28955"
                     /old_locus_tag="pgaptmp_005791"
     CDS             1392..1787
                     /gene="frdC"
                     /locus_tag="ACHKNP_RS28955"
                     /old_locus_tag="pgaptmp_005791"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGU4707682.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1798..2157
                     /gene="frdD"
                     /locus_tag="ACHKNP_RS28960"
                     /old_locus_tag="pgaptmp_005792"
     CDS             1798..2157
                     /gene="frdD"
                     /locus_tag="ACHKNP_RS28960"
                     /old_locus_tag="pgaptmp_005792"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU4707683.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            2220..3353
                     /locus_tag="ACHKNP_RS28965"
                     /old_locus_tag="pgaptmp_005793"
     CDS             2220..3353
                     /locus_tag="ACHKNP_RS28965"
                     /old_locus_tag="pgaptmp_005793"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU4707684.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            3442..3975
                     /gene="blc"
                     /locus_tag="ACHKNP_RS28970"
                     /old_locus_tag="pgaptmp_005794"
     CDS             3442..3975
                     /gene="blc"
                     /locus_tag="ACHKNP_RS28970"
                     /old_locus_tag="pgaptmp_005794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU4707685.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3972..4289)
                     /gene="sugE"
                     /locus_tag="ACHKNP_RS28975"
                     /old_locus_tag="pgaptmp_005795"
     CDS             complement(3972..4289)
                     /gene="sugE"
                     /locus_tag="ACHKNP_RS28975"
                     /old_locus_tag="pgaptmp_005795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU4707686.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(4471..4617)
                     /gene="ecnB"
                     /locus_tag="ACHKNP_RS28980"
                     /old_locus_tag="pgaptmp_005796"
     CDS             complement(4471..4617)
                     /gene="ecnB"
                     /locus_tag="ACHKNP_RS28980"
                     /old_locus_tag="pgaptmp_005796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU4707687.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(4728..4853)
                     /gene="ecnA"
                     /locus_tag="ACHKNP_RS28985"
                     /old_locus_tag="pgaptmp_005797"
     CDS             complement(4728..4853)
                     /gene="ecnA"
                     /locus_tag="ACHKNP_RS28985"
                     /old_locus_tag="pgaptmp_005797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGU4707688.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(4905..5471)
                     /gene="efp"
                     /locus_tag="ACHKNP_RS28990"
                     /old_locus_tag="pgaptmp_005798"
     CDS             complement(4905..5471)
                     /gene="efp"
                     /locus_tag="ACHKNP_RS28990"
                     /old_locus_tag="pgaptmp_005798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGU4707689.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            5513..6541
                     /gene="epmB"
                     /locus_tag="ACHKNP_RS28995"
                     /old_locus_tag="pgaptmp_005799"
     CDS             5513..6541
                     /gene="epmB"
                     /locus_tag="ACHKNP_RS28995"
                     /old_locus_tag="pgaptmp_005799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGU4707690.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1663 a         1719 c         1741 g         1535 t
ORIGIN      
        1 ctgcacggtg caaaccgtct gggttctaac tccctggcgg aactggtggt cttcggccgt
       61 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagcggca
      121 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctggttaa ccaggatggc
      181 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg caatggaaga aggttgcggt
      241 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
      301 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
      361 accattgaac tgggccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
      421 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
      481 gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
      541 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
      601 gatgcagccg ataaggcgga agcagccaat aagaaggaga aggcgaatgg ctgagatgaa
      661 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
      721 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
      781 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
      841 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
      901 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
      961 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
     1021 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
     1081 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
     1141 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
     1201 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggcg tatggagctg
     1261 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
     1321 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
     1381 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     1441 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     1501 tgtggttcag cattgaactg attttcgggc tgtttgccct gaaaaatggc ccggaagcct
     1561 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
     1621 tggcggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     1681 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     1741 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     1801 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
     1861 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
     1921 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
     1981 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     2041 cgtatgcacc acgcgatgca cgatctgaaa attcacgtgc ctgcgggcaa atgggttttc
     2101 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtatcgttac aatctaacgt
     2161 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
     2221 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttgctccaca tttgctgccc
     2281 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     2341 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
     2401 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     2461 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
     2521 taagcgatcc cacaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
     2581 cactattaca tctcgcaacc tacactgctg gcggcctgcc attgcaggtg ccggatgagg
     2641 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggcgccag
     2701 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     2761 cgtctggttt gagttttgag caggcgatgc aaacacgtgt cttccagcca ctcaaactca
     2821 accatacgtg gattaatgta ccgcccgcag aagaaaagaa ttacgcctgg ggatatcgcg
     2881 aaggcaaggc agttcatgtt tcgccagggg cgttagatgc tgaaacttat ggtgtgaagt
     2941 cgaccattga agatatggcc tgctgggtac gaagcaatat gaatccccgt gatatcaacg
     3001 acaaaacact tcagcaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
     3061 tgtatcaggg cctgggctgg gaaatgctgg actggccggt aaatcctgac agcatcatta
     3121 acggcagtgg caataaaatt gcactggcag cacaccccgt aaaagcgatt acgcccccaa
     3181 ctcctgcagt acgcgcatca tgggtacata aaacaggggc aaccggcgga tttggtagct
     3241 atgtcgcgtt tattccagaa aaagagctgg gtatagtgat gctggctaac aaaaactatc
     3301 ccaatccagc gagagtcgcc gccgcctggc agattctcaa cgctctacag taaaatttca
     3361 tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa
     3421 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
     3481 tttctggtcg ttgcctgcag ttctcctacg ccgccgcgtg gcgtaacagt agtgaataat
     3541 ttcgacgcca aacgttatct cggtacctgg tatgagattg cccgttttga tcaccgtttt
     3601 gaacgtggac tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg cggcctgaat
     3661 gtcattaata aaggctataa ccctgacaga ggaatgtggc agcagagtga agggaaagcg
     3721 tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat
     3781 ggcggttata acgttattgc actcgatcgg gaataccgcc atgcgctggt ttgcggcccg
     3841 gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag
     3901 gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag
     3961 cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc
     4021 agactcgcca ggcgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatggcc
     4081 gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctactgg taacgatttc
     4141 atcgcccagg caagtagcgc catactgaca atcatcgccg tcacggtaat aacactcggc
     4201 gtcaaacgac taaagccgtg ggtatatttc aggccaacgg cccataccac ttccagcaga
     4261 ccagcaataa ctaagataat ccaggacata tcaggctccg gagaattggg gccgtccccg
     4321 gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca
     4381 ggtggcaggg gaatttcaac tattacctta gtgactatcg cagaaaaaac aaacggcacg
     4441 acatacctgt cgtaccgtta ttgcttattg ctattgctgc gctttcgttg ctgcgccaga
     4501 aatcgcgtta ccgccatcag aaatgtcttc accaacgcca cgcgtggtgt tgcaggcagt
     4561 taatactgtt gaaagcacca gaacagaaaa gatcgctgca attgtcttct tcaccataac
     4621 gtcttccttt taggtaatgt tatttatgtt tgcctatagc aaattaataa tagacaacat
     4681 cgccaaaaat gacggaaatc tgatgatttt tcggaagaga agaaaaatta gctggcagcg
     4741 cgagagatgg agttgccgag atgtttgatg tcttcgccga aaccgcgagc ggtgttacag
     4801 cccgtgagca gcgtgctggc aagcaaaaca agaacgataa ggcgtttcat catctctgac
     4861 ctgaataagt gatggtgcag cctgcaggcc gcaccacaac cgcattactt cacgcgagag
     4921 acgtattcac cagagcgggt atccactttg atgacttcgc cgatttgtac gaacagcgga
     4981 accttaacca cagcgccagt agacagggta gccggtttgc cgccagtacc tgcggtatca
     5041 cctttcaggc ccggatcggt atcaacgatt tccagttcaa cgaagttcgg cggagtaacg
     5101 gagatcggct gaccattcca cagagttacg atacactctg cctgatccag cagccattta
     5161 gcgttgtcgc caattgcttt tgcatcagca gacagctgct cgaaagtttc gttgttcatg
     5221 aagtgccaga actcaccgtc gttgtacagg taagtcaggt tcatatcgac aacatcagcg
     5281 ccttcagcgg aatcagtaga tttgaaggtt ttttctacgc gagtaccggt cagcagacga
     5341 cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt ttacgaattc actcgcttca
     5401 accgcgtaag gttcgccgtc taacatgatt ttaagaccag cacgaaaatc gttgctatag
     5461 tacgttgcca taaggccctc tgaaatttgt taattggtag ctaagccaca aaatggcgca
     5521 tattgtaacc ctaaataccc catccagaga agattggtta acgcaacttg ccgatgttgt
     5581 gaccgatcct gatgaacttc tgcgtctttt gaatatagac gcggacgaaa aactgttggc
     5641 cggacgcagc gccaaaaagc tgtttgccct gcgtgtgccc cgctcattta tcgatcgcat
     5701 ggagaaaggc aatccggacg atcctctttt gcgtcaggta cttacctcgc aagatgagtt
     5761 tgtcgtcgcg tccggattct ccaccgaccc gctggaagaa cagcacagcg tagtgcctgg
     5821 tttgttgcat aaataccaca accgggcgct tttgctggtc aaaggcggct gcgcggtaaa
     5881 ttgccgctat tgcttccgtc gccacttccc ctatgccgaa aatcagggca acaagcgtaa
     5941 ctggcagact gctcttgagt atgttgctgc gcatccggaa ctggacgaga tgattttctc
     6001 cggcggcgat ccgctgatgg cgaaagatca cgagctggac tggttgctca cacaactgga
     6061 agccatcccg catatcaaac gtctgcggat tcacagccgt ctgccgattg tgatcccggc
     6121 acgtatcacc gaggcgctgg ttgaacgctt tgcccgttct acgctgcaaa tcttgctggt
     6181 gaatcacatc aatcatgcca atgaggtaga tgaaacgttc cgtcaggcga tggctaaatt
     6241 gcgccgtgtc ggtgtcaccc tgcttaacca gagcgttctg ttacgtggtg tgaacgataa
     6301 cgcacaaacg ctggcaaacc tgagtaatgc gttgttcgat gccggcgtaa tgccgtatta
     6361 cctgcatgtg ctcgataaag tacagggcgc ggcgcatttt atggtgagtg atgacgaagc
     6421 acggcagatt atgcgtgagt tgctgacact ggtgtcgggt tatctggtgc cgaaactggc
     6481 gcgagaaatc ggcggcgaac ccagcaaaac gccgctggat ctccagctac gccagcagta
     6541 actgattaaa cagggaagtt aaacattagc ctgatgcgct acgcttatca ggcctacatt
     6601 gtgcattgaa attgaatgga tttgcaatga actgtaggcc ggataaggcg tttacgcc
//
LOCUS       DAZFWL020000653         3598 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.guided.263, whole genome shotgun sequence.
ACCESSION   DAZFWL020000653 DAZFWL020000000
VERSION     DAZFWL020000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.guided.263"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..261
                     /locus_tag="ACHKNP_RS29000"
                     /old_locus_tag="pgaptmp_005800"
     CDS             <1..261
                     /locus_tag="ACHKNP_RS29000"
                     /old_locus_tag="pgaptmp_005800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HGU4707691.1"
                     /translation="PGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLLIA
                     WKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(318..1775)
                     /gene="pepD"
                     /locus_tag="ACHKNP_RS29005"
                     /old_locus_tag="pgaptmp_005801"
     CDS             complement(318..1775)
                     /gene="pepD"
                     /locus_tag="ACHKNP_RS29005"
                     /old_locus_tag="pgaptmp_005801"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU4707692.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2036..2494
                     /gene="gpt"
                     /locus_tag="ACHKNP_RS29010"
                     /old_locus_tag="pgaptmp_005802"
     CDS             2036..2494
                     /gene="gpt"
                     /locus_tag="ACHKNP_RS29010"
                     /old_locus_tag="pgaptmp_005802"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU4707693.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2586..>3598
                     /gene="frsA"
                     /locus_tag="ACHKNP_RS29015"
                     /old_locus_tag="pgaptmp_005803"
     CDS             2586..>3598
                     /gene="frsA"
                     /locus_tag="ACHKNP_RS29015"
                     /old_locus_tag="pgaptmp_005803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU4707694.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRV"
BASE COUNT          842 a          922 c          943 g          891 t
ORIGIN      
        1 ccgggcggtc aacatgtcaa taaaaccgac tcggcggtac gcgccacgca tttggcatcc
       61 ggtattagcg tgaaggttca gtcagagcgt agtcagcatg ctaacaagcg gctggcacga
      121 ttgctgattg cctggaagct ggagcagcag caacaggaaa atagcgcggt gctgaaatcg
      181 ctgcggcgaa tgttccatca ccagattgaa cgtggcaacc cgagacggac atttacaggg
      241 atggctttta tcgaaggata atgaaggatg aaactgccgg aaggcgatta aacgccatcc
      301 ggcagcaaat caaataatta cttcgccgga atttctttca gcagttcagt cagcagcgtc
      361 cagtaatgac ctacgctttc gatgtgaact tgctcatccg gagagtgtgg accggtgata
      421 gttggcccga tagaaaccat gtccatttcc ggatacggtt ttttgaacag accacattcc
      481 aggcccgcgt ggataatctg gatgttcggt gttttgttga acagacgctg ataggtttca
      541 cgtaccagat gcatcaccgg agaattagcg tccggctgcc agccaggata tgcgcctttc
      601 gcttcggttt tcgcgccagc tagtttaccc agcgaatcca gcatgctcac cacgtagtct
      661 ttaccgctgt cgatcagtga acggatcagg cagtgaattt ctacgttatt gtcagtcatg
      721 gtcaccacac cgacgttcag ggaggtttcg accacacctt ttgccacgtc ggaattgcgg
      781 atcacaccgt tcggggtggc gttcagcaga cgaataaagg tatcgcgaga tttcgcaatc
      841 agggcagctt tatcgttcgc tacagagtcc agcaacaagg ccagattttt ctctttctct
      901 gccagctcgt ttttcaggat ctcctgatag gtattcacca gagatttcag gacgtcgact
      961 ttatcagctg cgacagcaat ggtcgcaaag gcttcacgcg ggatggcgtt acgcagtgtg
     1021 ccgccgttga aatcgataag gcgcagatcc agttcttccg catgacccgc caggaagcgc
     1081 accagtagtt tgttggcatt acccaggcca acgtggattt caccgccgga gtgaccgcct
     1141 ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag ctggaaccgc ttcacgatct
     1201 aaatgcaggt tggaggtgaa gtcgataccc cccgcacaac ccatgtagat ttcaccttct
     1261 tcttcggagt cggtgttaat cagaatatca gcctgcaacc agttgctctg taagccgaac
     1321 gcaccgtcca taccggcttc ttcggtcatg gtcagcagca cttccagcgg gccgtgaacc
     1381 acgttttcgt cagccagaac cgccagcgca gacgccatac caatgccgtt atctgcaccc
     1441 agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat aaggctggat aggatctttc
     1501 gtgaagtcat gcacggtgtc gttatttttc tgcggcacca tatcgaggtg ggcctgtaag
     1561 acgaccggtt tacgattttc catacctgcg gtagcaggtt tacgaatcag gatattacct
     1621 acctgatcgc gttcgacatg gaaacctttc tcttttgccc aaccaacaat gtattcagcg
     1681 agttgctctt catgatagga cgggtgagga atagaacaga ttttggcaaa aatatcccac
     1741 agcggctgtg gagataattg agacagttca gacacgttaa gtctccttgt cgatcacccg
     1801 caaaacagta ttgcaggtca cagggttagc agaaaatgtt gtcaacacaa gacaggcttg
     1861 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
     1921 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
     1981 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
     2041 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     2101 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctggtacc
     2161 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
     2221 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
     2281 aggcttcatc gttattgatg acctggtgga taccggtggt actgctgttg cgattcgtga
     2341 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
     2401 tgatgactat gttgttgata tcccgcaaga tacctggatc gaacagccgt gggatatggg
     2461 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
     2521 cgttgttctt tttaacttca ggcaggttac aatagtttcc agtaagtatt ctggaggctg
     2581 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
     2641 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggctc
     2701 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
     2761 gcggtattga cccacgtgaa atcctcgacg tccaggcacg tattgtgatg agcgatgctg
     2821 aacgtaccga cgatgattta tacgatactg tgattggcta ccgtggtggc aactggattt
     2881 atgagtgggc cacccaggcg atggtgtggc aacaaaaagc ctgtgcggaa gaagatccgc
     2941 aactcagtgg tcgacactgg ctgcatgcgg ctacgttgta caacattgcc gcctatcctc
     3001 atctgaaagg agatgacctg gccgagcaag cgcaggcttt atcaaaccgc gcctatgaag
     3061 aggccgccca gcgtctgccg ggcacgatgc ggcagatgga gtttaccgta cccggcggtg
     3121 cgcccatcac cggctttttg catatgccga aaggcgatgg cccgttcccg acagtattaa
     3181 tgtgtggtgg tctggatgcg atgcagacgg actattacag cctgtatgaa cgttattttg
     3241 cgccgcgcgg cattgcgatg ctgactattg atatgccatc ggtgggcttt tcttcaaaat
     3301 ggaagctcac ccaggactcc agcctgttgc atcagcacgt cttaaaggcg ctgcctaacg
     3361 taccgtgggt ggatcacact cgcgtcgcgg cctttggttt ccgtttcggc gctaacgttg
     3421 ccgtgcgtct ggcatatctt gaatcgccgc gtctgaaagc ggttgcctgt cttggtccgg
     3481 tagttcatac cctgttgagt gattttaagt gccagcaaca ggtgccggaa atgtatcttg
     3541 acgttctggc gagtcgtttg gggatgcatg atgcttccga tgacgcgtta cgcgtgga
//
LOCUS       DAZFWL020000654         2964 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.guided.282, whole genome shotgun sequence.
ACCESSION   DAZFWL020000654 DAZFWL020000000
VERSION     DAZFWL020000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.guided.282"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..184)
                     /locus_tag="ACHKNP_RS29020"
                     /old_locus_tag="pgaptmp_005804"
     CDS             complement(<1..184)
                     /locus_tag="ACHKNP_RS29020"
                     /old_locus_tag="pgaptmp_005804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707695.1"
                     /translation="MKNKSPDLWLLFYLFSRCILLISILSFSGILFGQVVSFFKAGSH
                     ISFSKDDIYFIVKIGCG"
     gene            complement(174..422)
                     /locus_tag="ACHKNP_RS29025"
                     /old_locus_tag="pgaptmp_005805"
     CDS             complement(174..422)
                     /locus_tag="ACHKNP_RS29025"
                     /old_locus_tag="pgaptmp_005805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HGU4707696.1"
                     /translation="MGAYLGSKVKGEDPTVPMVGNAIGTVLGNKAGEKFTKEMLSKGY
                     SPVTSDVIGTVSGGTMTIGTAIDFGIEESQKKGDSNEK"
     gene            786..980
                     /locus_tag="ACHKNP_RS29030"
                     /old_locus_tag="pgaptmp_005806"
     CDS             786..980
                     /locus_tag="ACHKNP_RS29030"
                     /old_locus_tag="pgaptmp_005806"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-zinc-cadmium resistance protein"
                     /protein_id="HGU4707697.1"
                     /translation="MVFVATQHGQKVEFTPQPVKLGQSSGDSQWIEVVNGLNQGQKYA
                     AQGSFLLKSEMEKGEASHEH"
     gene            970..1806
                     /locus_tag="ACHKNP_RS29035"
                     /old_locus_tag="pgaptmp_005807"
     CDS             970..1806
                     /locus_tag="ACHKNP_RS29035"
                     /old_locus_tag="pgaptmp_005807"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HGU4707698.1"
                     /translation="MSTKSPHIPDHESVKPEGLFDRLIQFSIHNAIWVMLFVAAWIAI
                     GIYSYQKLPIDAVPDITNTQVQINTSANGFTALEMEQRITYPIENAMSGMPKMEQTRS
                     ISRYGLSQVTIIFEDGTDIYWARQLINQRLQEAMGEMPEDVQPNMSPISTGLGEIYQW
                     VIKAEPNAKKPDGTPYSAMDLREIQDWIVRPQLQRVKGVAEINSIGGFNKTYVVAPDL
                     NRLQQLQIPLSDLQTALTENNENRGAGYIEKNGQQLTVRVPHSIVNKTFLSFIFNGLF
                     SC"
     gene            2047..2862
                     /gene="sul2"
                     /locus_tag="ACHKNP_RS29040"
                     /old_locus_tag="pgaptmp_005808"
     CDS             2047..2862
                     /gene="sul2"
                     /locus_tag="ACHKNP_RS29040"
                     /old_locus_tag="pgaptmp_005808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HGU4707699.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          772 a          739 c          683 g          770 t
ORIGIN      
        1 ccccacaacc tattttaaca ataaaatata tatcatcctt agaaaaagat atatgactac
       61 cggctttaaa gaaactgacc acttgtccaa aaagaatccc agaaaagcta agtatagata
      121 ttagcaatat acaccttgaa aaaagataga ataacagcca taagtcagga gatttatttt
      181 tcattactat ctcctttctt ctgagattct tcaatgccga agtcgatagc tgtacctata
      241 gtcatagtcc ccccagagac agttccaata acatcagaag ttactggcga gtatcccttc
      301 gatagcattt ctttggtgaa tttctctccg gctttgttac ccagaacagt acctattgca
      361 ttccccacca ttggcactgt agggtcttcg cctttaactt tacttcctaa gtaagctccc
      421 attgtgttaa taaatgtgct gaaaatgaca cttttaccct gtgtaagccc accttcacct
      481 gcggcaataa gagtatccgt agcactatat ttctcacccg gcttaagggt taaatactgc
      541 ttaatgccat ctgttacacc tcctaaaagg ctacccccaa taacagcaac ggtacccgcg
      601 gctgggacta taagttcgcc accaagaaca gaagcacctg ctccccatgc aatcagtaaa
      661 cctctgacag gatcctgtcc ttccggaaga ttacctgttg caagtttatc agaagcatac
      721 ttaattgtga aagtaccgca ttacgggtac ttaagtccgc agttcagaat gtcgatggaa
      781 aagatgtggt ttttgtcgcc actcagcatg gacaaaaagt tgaattcact ccgcagccgg
      841 tgaaattagg tcaatcgtct ggggatagcc agtggattga agtggtaaat gggcttaatc
      901 agggacaaaa atatgccgct caaggcagtt tcttactgaa atctgagatg gaaaaaggag
      961 aggcttcaca tgagcactaa aagccctcac attccagacc acgaatcggt aaaacctgaa
     1021 gggctgtttg atcggctgat ccagttttct attcataacg caatctgggt catgctgttt
     1081 gttgcagcct ggattgctat tggtatttat agttatcaaa agttaccgat tgatgctgta
     1141 ccggatatta ccaatactca ggtgcagatt aacacttcag ccaatggctt tacggctttg
     1201 gaaatggaac agcggattac ctatccaatt gagaatgcca tgtcaggcat gccgaaaatg
     1261 gaacaaaccc gttcgatttc acgctacggc ttatcgcaag tgaccatcat ttttgaagat
     1321 ggcacggata tttactgggc cagacagctt atcaaccagc gcttgcaaga agcaatggga
     1381 gaaatgcctg aagatgtgca acctaacatg tcgcctattt cgacaggttt aggtgaaatc
     1441 tatcaatggg tgattaaagc cgaaccaaat gctaaaaagc cggatggaac cccttattcg
     1501 gcgatggatt tacgtgaaat tcaggattgg attgtaagac cgcaattaca gcgcgtaaaa
     1561 ggcgttgcgg agatcaacag tatcggtggc tttaataaaa cctatgtggt tgcccctgat
     1621 ctgaaccgat tgcaacaatt gcaaattcct ttgtctgatt tacaaactgc cttaacagag
     1681 aataatgaaa accgtggtgc tggctatatt gagaaaaatg gacaacagct aacggttcgt
     1741 gttccccact ctattgtaaa caagacattt ttatctttta tattcaatgg cttattttcc
     1801 tgctaattgg taataccatg aaaaatacca tgctcagaaa aggcttaaca atattttgaa
     1861 aaattgccta ctgagcgctg ccgcacagct ccataggccg ctttcctggc tttgcttcca
     1921 gatgtatgct attctgctcc tgcagctaat ggatcaccgc aaacaggtta ctcgcctggg
     1981 gattcccttt cgacccgatc atccgtatga gactcatgct cgattattat tattatagaa
     2041 gcccccatga ataaatcgct catcattttc ggcatcgtca acataacctc ggacagtttc
     2101 tccgatggag gccggtatct ggcgccagac gcagccattg cgcaggcgcg taagctgatg
     2161 gccgaggggg cagatgtgat cgacctcggt ccggcatcca gcaatcccga cgccgcgcct
     2221 gtttcgtccg acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat
     2281 ggcattcccg tctcgctcga cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt
     2341 ggtgtggcct atctcaatga tattcgcggt tttccagacg ctgcgttcta tccgcaattg
     2401 gcgaaatcat ctgccaaact cgtcgttatg cattcggtgc aagacgggca ggcagatcgg
     2461 cgcgaggcac ccgctggcga catcatggat cacattgcgg cgttctttga cgcgcgcatc
     2521 gcggcgctga cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc cggcatgggg
     2581 ttttttctgg gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg
     2641 cggctgcgct tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg
     2701 ctcacaggcc gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc
     2761 gccgcaggtg gagctgactt catccgcaca cacgagccgc gccccttgcg cgacgggctg
     2821 gcggtattgg cggcgctgaa agaaaccgca agaattcgtt aactgcacat tcgggatatt
     2881 tctctatatt cgcggttcag caggcatgtc ccctttgagg gcgacccgac gacaggataa
     2941 tcgaccttat ggtgcgcaaa tatt
//
LOCUS       DAZFWL020000655         2615 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.guided.293, whole genome shotgun sequence.
ACCESSION   DAZFWL020000655 DAZFWL020000000
VERSION     DAZFWL020000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.guided.293"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            266..1069
                     /locus_tag="ACHKNP_RS29045"
                     /old_locus_tag="pgaptmp_005809"
                     /note="aph(3'')-Ib"
     CDS             266..1069
                     /locus_tag="ACHKNP_RS29045"
                     /old_locus_tag="pgaptmp_005809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HGU4707700.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            1069..1905
                     /locus_tag="ACHKNP_RS29050"
                     /old_locus_tag="pgaptmp_005810"
     CDS             1069..1905
                     /locus_tag="ACHKNP_RS29050"
                     /old_locus_tag="pgaptmp_005810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HGU4707701.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(2289..>2615)
                     /locus_tag="ACHKNP_RS29055"
                     /old_locus_tag="pgaptmp_005811"
     CDS             complement(2289..>2615)
                     /locus_tag="ACHKNP_RS29055"
                     /old_locus_tag="pgaptmp_005811"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4707702.1"
                     /translation="PDDRIIDLMVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVF
                     RRGDHRHRVVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSP
                     TALDRS"
BASE COUNT          570 a          695 c          750 g          600 t
ORIGIN      
        1 ttttcgcgaa accatttgat catatgacaa gatgtgtatc taccttaact taatgatttt
       61 gataaaaatc attaggggat tcatcagcac tcggctggaa atcgccggtg gcatttgaga
      121 aaaaagccgc ttaaaatgag agatagaccg gaacacaacc ggtgcaagac catggaaagc
      181 atgttctcgg acttacgtag caactcgttt cttttcgcag gttgagccac ctccgcgctt
      241 catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg gtgaatcgca
      301 ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc gacgtggtga
      361 cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg ctggagagcg
      421 tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtga tcaactggca
      481 ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac cggcggctga
      541 tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac ttggcgctgt
      601 tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa tgttcggacg
      661 cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg acgaggacaa
      721 gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg tgcggctcga
      781 ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga acttcatggt
      841 ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg gaacagcaga
      901 tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg cagcgccaga
      961 tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag cccccgaccg
     1021 cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat gttcatgccg
     1081 cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga caccttttcc
     1141 agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa gggattgaaa
     1201 cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg gcgcaatggg
     1261 aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga atatgccggg
     1321 gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac cgaaattgca
     1381 gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc tgcccttctc
     1441 ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca aaacgcaggt
     1501 tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag caatgcctcg
     1561 gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc cagtcgcggc
     1621 tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc cgccaatatg
     1681 ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat tgcacagatg
     1741 gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga ccaggcgtac
     1801 gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca acgcgatcta
     1861 gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actagatatc aagcgacttc
     1921 tcctatcccc tgggaacaca tcaatcttac cggagaatat cgttggccaa agccttagcg
     1981 taggatttcg ccctctcccg caaacgaccc catccttgat cacctgaagc acaaagccga
     2041 aaccagcggg accagggcgt tacccgaaag ccgggcgcca ccggctgagc tgctcctggg
     2101 tctgtttgac tgacgagcct gaaggccaac gataccaatc aaaatgctgc gttcacagcg
     2161 ccgcggcagg gatccgccgt gctggttgtc ggaaaaggag ccgctagtgg gaaagaggag
     2221 ggtaaatttt cagcgttgct ggctccccgt cagccggatt gggttgcatc gcaggggtgt
     2281 cgaaagagtc aactgcggtc caaagctgtt ggacttgggt gaaaagggcg tttattcttc
     2341 ctatacgttg tcggcagcgg gccaaaaagg aatacgtcca tgcccatcga ggtgaaaccg
     2401 gctgtgagcg cgggttcaag catatagccc gacaggcgcg tatccttgcc gatcacgaca
     2461 cgatggcggt ggtcaccgcg acgaaagaca cggccagccg ccatgccgac gcgcaaggcg
     2521 gtttccgccg tcatcgcgcc ttcgttggct ttgccacgaa taccgtctgt gccgaaatat
     2581 ttgcgcacca taaggtcgat tatcctgtcg tcggg
//
LOCUS       DAZFWL020000656         1931 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.guided.343, whole genome shotgun sequence.
ACCESSION   DAZFWL020000656 DAZFWL020000000
VERSION     DAZFWL020000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.guided.343"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            127..1086
                     /gene="stxA2"
                     /locus_tag="ACHKNP_RS29060"
                     /old_locus_tag="pgaptmp_005812"
     CDS             127..1086
                     /gene="stxA2"
                     /locus_tag="ACHKNP_RS29060"
                     /old_locus_tag="pgaptmp_005812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A, a/c/d subtype"
                     /protein_id="HGU4707703.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLIRKSQFLYTTGK"
     gene            1098..1367
                     /locus_tag="ACHKNP_RS29065"
                     /old_locus_tag="pgaptmp_005813"
     CDS             1098..1367
                     /locus_tag="ACHKNP_RS29065"
                     /old_locus_tag="pgaptmp_005813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU4707704.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(1665..>1931)
                     /gene="iraM"
                     /locus_tag="ACHKNP_RS29070"
                     /old_locus_tag="pgaptmp_005814"
     CDS             complement(1665..>1931)
                     /gene="iraM"
                     /locus_tag="ACHKNP_RS29070"
                     /old_locus_tag="pgaptmp_005814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU4707705.1"
                     /translation="AIWCNIKLILWYQSDIFLPPGCIFTPTRTGVILNNKELPVTIYN
                     VTPFNKNVWNLIKSSQECPANTGNITDKCFNHRCILEICPYGRK"
BASE COUNT          510 a          386 c          477 g          558 t
ORIGIN      
        1 aatcggtcac tggttcgaat ccagtacaac gcgccatatt tatttaccag gctcgctttt
       61 gcgggccttt tttatatctg cgccgggtct ggtgctgatt acttcagcca aaaggaacac
      121 ctgtatatga agtgtatatt atttaaatgg gtactgtgcc tgttactggg tttttcttcg
      181 gtatcctatt cccgggaatt tacgatagac ttttcgactc aacaaagtta tgtctcttcg
      241 ttaaatagta tacggacaga gatatcgacc cctcttgaac atatatctca ggggaccaca
      301 tcggtgtctg ttattaacca caccccgccg ggcagttatt ttgctgtgga tatacgaggg
      361 cttgatgtct atcaggcgcg ttttgaccat cttcgtctga ttattgagca aaataattta
      421 tatgtggccg ggttcgttaa tacggcaaca aatactttct accgtttttc agattttaca
      481 catatatcag tgcccgatgt gacaacggtt tccatgacaa cggacagcag ttataccact
      541 ctgcaacgtg tcgcagcgct ggaacgttcc ggaatgcaaa tcagtcgtca ctcactggtt
      601 tcatcatatc tggcgttaat ggagttcagt ggtaatacaa tgaccagaga tgcatccaga
      661 gcagttctgc gttttgtcac tgtcacggca gaagccttac gcttcaggca gatacagaga
      721 gaatttcgtc aggcactgtc tgaaactgct cctgtgtata cgatgacgcc gggagacgtg
      781 gacctcactc tgaactgggg gcgaatcagc aatgtgcttc cggagtatcg gggagaggat
      841 ggtgtcagag tggggagaat atcctttaat aatatatcag cgatactggg tactgtggcc
      901 gttatactga attgccatca tcagggggcg cgttctgttc gcgccgtgaa tgaagagagt
      961 caaccagaat gtcagataac tggcgacagg cccgttataa aaataaacaa tacattatgg
     1021 gaaagtaata cagctgcagc gtttctgatc agaaagtcac agtttttata tacaacgggt
     1081 aaataaagga gttaagtatg aagaagatgt ttatggcggt tttatttgca ttagtttctg
     1141 ttaatgcaat ggcggcggat tgtgctaaag gtaaaattga gttttccaag tataatgagg
     1201 atgacacatt tacagtgaag gttgacggga aagaatactg gaccagtcgc tggaatctgc
     1261 aaccgttact gcaaagtgct cagttgacag gaatgactgt cacaatcaaa tccagtacct
     1321 gtgaatcagg ctccggattt gctgaagtgc agtttaataa tgactgaggc ataacctgat
     1381 tcgtggtatg tgggtaacaa gtgtaatctg tgtcacaatt cagtcagttg acagttgcct
     1441 gtcagactga gcatttgtta aaaaaatttc gcatggtgaa tccccctgtg cggaggggcg
     1501 actggtgtat aaggtgtcat ctctgacgac aacctggatc gagatacgcg ggttcaggga
     1561 caccggactg aactcaccgg gaggcacccg gcaccatgtt catggtgata cagaaatgcg
     1621 gcttcagccc ctctccggag gggcttttta tctgaatgat tctgttattt tcgcccgtat
     1681 gggcatattt ccagaatgca gcgatgatta aaacacttat ctgtgatatt tcctgtgttt
     1741 gcagggcatt cctggctgct tttaattaaa ttccagacgt ttttattaaa tggggtcacg
     1801 ttgtaaatgg tgacggggag ctctttatta ttgagtatta cgcctgtccg ggttggtgta
     1861 aaaatacagc ccggagggag gaagatatca gactgatacc ataatattaa ttttatgtta
     1921 caccatatgg c
//
LOCUS       DAZFWL020000657         1846 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212F71163
            SAMN43283583-rid25106993.guided.348, whole genome shotgun sequence.
ACCESSION   DAZFWL020000657 DAZFWL020000000
VERSION     DAZFWL020000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283583
            Sequence Read Archive: SRR30325304
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351566.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351566.2
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:22:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,816
            CDSs (total)                      :: 5,714
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 5 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 109 of 258
            Pseudo Genes (incomplete)         :: 137 of 258
            Pseudo Genes (internal stop)      :: 62 of 258
            Pseudo Genes (multiple problems)  :: 45 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283583-rid25106993.guided.348"
                     /serotype="O 26:H11"
                     /isolate="2212F71163"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-09"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            62..1267
                     /gene="tet(B)"
                     /locus_tag="ACHKNP_RS29075"
                     /old_locus_tag="pgaptmp_005815"
     CDS             62..1267
                     /gene="tet(B)"
                     /locus_tag="ACHKNP_RS29075"
                     /old_locus_tag="pgaptmp_005815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HGU4707706.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1380..>1846)
                     /locus_tag="ACHKNP_RS29080"
                     /old_locus_tag="pgaptmp_005816"
     CDS             complement(1380..>1846)
                     /locus_tag="ACHKNP_RS29080"
                     /old_locus_tag="pgaptmp_005816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HGU4707707.1"
                     /translation="LVTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWS
                     ALESIFEHYLDYIIDNNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQEN
                     QLKAYDDDVLKNLLNGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          477 a          332 c          399 g          638 t
ORIGIN      
        1 tgacactcta tcattgatag agttatttta ccactcacta tcagtgatag agaaaagtga
       61 aatgaatagt tcgacaaaga tcgcattggt aattacgtta ctcgatgcca tggggattgg
      121 ccttatcatg ccagtcttgc caacgttatt acgtgaattt attgcttcgg aagatatcgc
      181 taaccacttt ggcgtattgc ttgcacttta tgcgttaatg caggttatct ttgctccttg
      241 gcttggaaaa atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt cattaatagg
      301 cgcatcgctg gattacttat tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg
      361 ccgtttgctt tcagggatca caggagctac tggggctgtc gcggcatcgg tcattgccga
      421 taccacctca gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct
      481 tggtttaata gcggggccta ttattggtgg ttttgcagga gagatttcac cgcatagtcc
      541 cttttttatc gctgcgttgc taaatattgt cactttcctt gtggttatgt tttggttccg
      601 tgaaaccaaa aatacacgtg ataatacaga taccgaagta ggggttgaga cgcaatcgaa
      661 ttcggtatac atcactttat ttaaaacgat gcccattttg ttgattattt atttttcagc
      721 gcaattgata ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg
      781 atggaatagc atgatggttg gcttttcatt agcgggtctt ggtcttttac actcagtatt
      841 ccaagccttt gtggcaggaa gaatagccac taaatggggc gaaaaaacgg cagtactgct
      901 cggatttatt gcagatagta gtgcatttgc ctttttagcg tttatatctg aaggttggtt
      961 agttttccct gttttaattt tattggctgg tggtgggatc gctttacctg cattacaggg
     1021 agtgatgtct atccaaacaa agagtcatca gcaaggtgct ttacagggat tattggtgag
     1081 ccttaccaat gcaaccggtg ttattggccc attactgttt gctgttattt ataatcattc
     1141 actaccaatt tgggatggct ggatttggat tattggttta gcgttttact gtattattat
     1201 cctgctatcg atgaccttca tgttaacccc tcaagctcag gggagtaaac aggagacaag
     1261 tgcttagtta tttcgtcacc aaatgatgtt attccgcgaa atataatgac cctcttgata
     1321 acccaagagg gcatttttta cgataaagaa gatttagctt caaataaaac ctatctattt
     1381 tatttatctt tcaagctcaa taaaaagccg cggtaaatag caataaattg gcctttttta
     1441 tcggcaagct cttttaggtt tttcgcatgt attgcgatat gcataaacca gccattgagt
     1501 aagtttttaa gcacatcatc atcataagct ttaagttggt tctcttggat caatttgctg
     1561 acaatggcgt ttaccttacc agtaatgtat tcaaggctaa ttttttcaag ttcattccaa
     1621 ccaatgatag gcatcacttc ttggataggg ataaggtttt tattattatc aataatataa
     1681 tcaagataat gttcaaatat actttctaag gcagaccaac catttgttaa atcagttttt
     1741 gttgtgatgt aggcatcaat cataattaat tgctgcttat aacaggcact gagtaattgt
     1801 tttttatttt taaagtgatg ataaaaggca cctttggtca ccaacg
//
